LOCUS       DBGJFU010000001       104682 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.001, whole genome shotgun sequence.
ACCESSION   DBGJFU010000001 DBGJFU010000000
VERSION     DBGJFU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 104682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.001"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            67..183
                     /gene="rrf"
                     /locus_tag="ACOIAS_000001"
     rRNA            67..183
                     /gene="rrf"
                     /locus_tag="ACOIAS_000001"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            303..494
                     /locus_tag="ACOIAS_000002"
     CDS             303..494
                     /locus_tag="ACOIAS_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HGZ2699737.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            649..2058
                     /locus_tag="ACOIAS_000003"
     CDS             649..2058
                     /locus_tag="ACOIAS_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2699738.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2189..2878
                     /locus_tag="ACOIAS_000004"
     CDS             2189..2878
                     /locus_tag="ACOIAS_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGZ2699739.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2881..3816
                     /locus_tag="ACOIAS_000005"
     CDS             2881..3816
                     /locus_tag="ACOIAS_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2699740.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMNGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3813..4706
                     /locus_tag="ACOIAS_000006"
     CDS             3813..4706
                     /locus_tag="ACOIAS_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HGZ2699741.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4784..5530
                     /locus_tag="ACOIAS_000007"
     CDS             4784..5530
                     /locus_tag="ACOIAS_000007"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HGZ2699742.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5531..6364
                     /gene="rsmI"
                     /locus_tag="ACOIAS_000008"
     CDS             5531..6364
                     /gene="rsmI"
                     /locus_tag="ACOIAS_000008"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGZ2699743.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFIFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6387..7091
                     /locus_tag="ACOIAS_000009"
     CDS             6387..7091
                     /locus_tag="ACOIAS_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HGZ2699744.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7279..7707)
                     /locus_tag="ACOIAS_000010"
     CDS             complement(7279..7707)
                     /locus_tag="ACOIAS_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HGZ2699745.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7744..9174)
                     /locus_tag="ACOIAS_000011"
     CDS             complement(7744..9174)
                     /locus_tag="ACOIAS_000011"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGZ2699746.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9306..9890
                     /locus_tag="ACOIAS_000012"
     CDS             9306..9890
                     /locus_tag="ACOIAS_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HGZ2699747.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9906..10427
                     /locus_tag="ACOIAS_000013"
     CDS             9906..10427
                     /locus_tag="ACOIAS_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HGZ2699748.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10493..10739
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10823..12760
                     /gene="metG"
                     /locus_tag="ACOIAS_000014"
     CDS             10823..12760
                     /gene="metG"
                     /locus_tag="ACOIAS_000014"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGZ2699749.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12790..13560
                     /locus_tag="ACOIAS_000015"
     CDS             12790..13560
                     /locus_tag="ACOIAS_000015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGZ2699750.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13897..14328
                     /gene="phnG"
                     /locus_tag="ACOIAS_000016"
     CDS             13897..14328
                     /gene="phnG"
                     /locus_tag="ACOIAS_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGZ2699751.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14343..14918
                     /gene="phnH"
                     /locus_tag="ACOIAS_000017"
     CDS             14343..14918
                     /gene="phnH"
                     /locus_tag="ACOIAS_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGZ2699752.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14921..16006
                     /locus_tag="ACOIAS_000018"
     CDS             14921..16006
                     /locus_tag="ACOIAS_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HGZ2699753.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16026..16895
                     /locus_tag="ACOIAS_000019"
     CDS             16026..16895
                     /locus_tag="ACOIAS_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGZ2699754.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16888..17754
                     /locus_tag="ACOIAS_000020"
     CDS             16888..17754
                     /locus_tag="ACOIAS_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2699755.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17795..18508
                     /locus_tag="ACOIAS_000021"
     CDS             17795..18508
                     /locus_tag="ACOIAS_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGZ2699756.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18509..19678
                     /locus_tag="ACOIAS_000022"
     CDS             18509..19678
                     /locus_tag="ACOIAS_000022"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGZ2699757.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19682..20518
                     /locus_tag="ACOIAS_000023"
     CDS             19682..20518
                     /locus_tag="ACOIAS_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HGZ2699758.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20519..21514
                     /locus_tag="ACOIAS_000024"
     CDS             20519..21514
                     /locus_tag="ACOIAS_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2699759.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21532..23220
                     /locus_tag="ACOIAS_000025"
     CDS             21532..23220
                     /locus_tag="ACOIAS_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HGZ2699760.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGTSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23232..24299
                     /locus_tag="ACOIAS_000026"
     CDS             23232..24299
                     /locus_tag="ACOIAS_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2699761.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDVMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24736..25731
                     /locus_tag="ACOIAS_000027"
     CDS             24736..25731
                     /locus_tag="ACOIAS_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2699762.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            25749..27437
                     /locus_tag="ACOIAS_000028"
     CDS             25749..27437
                     /locus_tag="ACOIAS_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HGZ2699763.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            27449..28516
                     /locus_tag="ACOIAS_000029"
     CDS             27449..28516
                     /locus_tag="ACOIAS_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2699764.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            28708..29238
                     /gene="rnmV"
                     /locus_tag="ACOIAS_000030"
     CDS             28708..29238
                     /gene="rnmV"
                     /locus_tag="ACOIAS_000030"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HGZ2699765.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            29409..30278
                     /gene="rsmA"
                     /locus_tag="ACOIAS_000031"
     CDS             29409..30278
                     /gene="rsmA"
                     /locus_tag="ACOIAS_000031"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGZ2699766.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            30344..31609
                     /locus_tag="ACOIAS_000032"
     CDS             30344..31609
                     /locus_tag="ACOIAS_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2699767.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIDEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            31760..32875
                     /locus_tag="ACOIAS_000033"
     CDS             31760..32875
                     /locus_tag="ACOIAS_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2699768.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            33061..33957
                     /locus_tag="ACOIAS_000034"
     CDS             33061..33957
                     /locus_tag="ACOIAS_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HGZ2699769.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            34027..34824
                     /locus_tag="ACOIAS_000035"
     CDS             34027..34824
                     /locus_tag="ACOIAS_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2699770.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(34879..36231)
                     /gene="murC"
                     /locus_tag="ACOIAS_000036"
     CDS             complement(34879..36231)
                     /gene="murC"
                     /locus_tag="ACOIAS_000036"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGZ2699771.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            36450..37328
                     /gene="purR"
                     /locus_tag="ACOIAS_000037"
     CDS             36450..37328
                     /gene="purR"
                     /locus_tag="ACOIAS_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HGZ2699772.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            37484..37762
                     /gene="spoVG"
                     /locus_tag="ACOIAS_000038"
     CDS             37484..37762
                     /gene="spoVG"
                     /locus_tag="ACOIAS_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HGZ2699773.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            37975..39354
                     /gene="glmU"
                     /locus_tag="ACOIAS_000039"
     CDS             37975..39354
                     /gene="glmU"
                     /locus_tag="ACOIAS_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGZ2699774.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            39449..40399
                     /locus_tag="ACOIAS_000040"
     CDS             39449..40399
                     /locus_tag="ACOIAS_000040"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGZ2699775.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      41243..41328
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            41490..42011
                     /locus_tag="ACOIAS_000041"
     CDS             41490..42011
                     /locus_tag="ACOIAS_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HGZ2699776.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTTTDKAGLTILETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTDTEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDELTDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            42163..43839
                     /locus_tag="ACOIAS_000042"
     CDS             42163..43839
                     /locus_tag="ACOIAS_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HGZ2699777.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            43851..45059
                     /locus_tag="ACOIAS_000043"
     CDS             43851..45059
                     /locus_tag="ACOIAS_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HGZ2699778.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            45074..46786
                     /locus_tag="ACOIAS_000044"
     CDS             45074..46786
                     /locus_tag="ACOIAS_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HGZ2699779.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            46779..47687
                     /gene="pilO"
                     /locus_tag="ACOIAS_000045"
     CDS             46779..47687
                     /gene="pilO"
                     /locus_tag="ACOIAS_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HGZ2699780.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            47714..48280
                     /locus_tag="ACOIAS_000046"
     CDS             47714..48280
                     /locus_tag="ACOIAS_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGZ2699781.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            48317..48844
                     /locus_tag="ACOIAS_000047"
     CDS             48317..48844
                     /locus_tag="ACOIAS_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGZ2699782.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            48865..50403
                     /locus_tag="ACOIAS_000048"
     CDS             48865..50403
                     /locus_tag="ACOIAS_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2699783.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRNSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            50503..51585
                     /locus_tag="ACOIAS_000049"
     CDS             50503..51585
                     /locus_tag="ACOIAS_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGZ2699784.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            51566..52348
                     /locus_tag="ACOIAS_000050"
     CDS             51566..52348
                     /locus_tag="ACOIAS_000050"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGZ2699785.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            52630..53100
                     /locus_tag="ACOIAS_000051"
     CDS             52630..53100
                     /locus_tag="ACOIAS_000051"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HGZ2699786.1"
                     /translation="MVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNIVQ
                     FDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFILA
                     FLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            53114..53674
                     /gene="pth"
                     /locus_tag="ACOIAS_000052"
     CDS             53114..53674
                     /gene="pth"
                     /locus_tag="ACOIAS_000052"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGZ2699787.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            53691..57077
                     /gene="mfd"
                     /locus_tag="ACOIAS_000053"
     CDS             53691..57077
                     /gene="mfd"
                     /locus_tag="ACOIAS_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGZ2699788.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            57108..58103
                     /locus_tag="ACOIAS_000054"
     CDS             57108..58103
                     /locus_tag="ACOIAS_000054"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2699789.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            58227..58766
                     /gene="spoVT"
                     /locus_tag="ACOIAS_000055"
     CDS             58227..58766
                     /gene="spoVT"
                     /locus_tag="ACOIAS_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HGZ2699790.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            58886..60499
                     /locus_tag="ACOIAS_000056"
     CDS             58886..60499
                     /locus_tag="ACOIAS_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGZ2699791.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            60606..62054
                     /gene="mazG"
                     /locus_tag="ACOIAS_000057"
     CDS             60606..62054
                     /gene="mazG"
                     /locus_tag="ACOIAS_000057"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGZ2699792.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            62143..62421
                     /locus_tag="ACOIAS_000058"
     CDS             62143..62421
                     /locus_tag="ACOIAS_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGZ2699793.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            62493..62735
                     /locus_tag="ACOIAS_000059"
     CDS             62493..62735
                     /locus_tag="ACOIAS_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HGZ2699794.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYKILE"
     gene            62797..63051
                     /locus_tag="ACOIAS_000060"
     CDS             62797..63051
                     /locus_tag="ACOIAS_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HGZ2699795.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            63052..63585
                     /gene="yabQ"
                     /locus_tag="ACOIAS_000061"
     CDS             63052..63585
                     /gene="yabQ"
                     /locus_tag="ACOIAS_000061"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HGZ2699796.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            63591..63875
                     /locus_tag="ACOIAS_000062"
     CDS             63591..63875
                     /locus_tag="ACOIAS_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HGZ2699797.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            63988..64908
                     /locus_tag="ACOIAS_000063"
     CDS             63988..64908
                     /locus_tag="ACOIAS_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HGZ2699798.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            64998..67370
                     /gene="spoIIE"
                     /locus_tag="ACOIAS_000064"
     CDS             64998..67370
                     /gene="spoIIE"
                     /locus_tag="ACOIAS_000064"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HGZ2699799.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            67721..69613
                     /gene="pepF"
                     /locus_tag="ACOIAS_000065"
     CDS             67721..69613
                     /gene="pepF"
                     /locus_tag="ACOIAS_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGZ2699800.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            69727..70692
                     /gene="galU"
                     /locus_tag="ACOIAS_000066"
     CDS             69727..70692
                     /gene="galU"
                     /locus_tag="ACOIAS_000066"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGZ2699801.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            71192..72796
                     /gene="rho"
                     /locus_tag="ACOIAS_000067"
     CDS             71192..72796
                     /gene="rho"
                     /locus_tag="ACOIAS_000067"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGZ2699802.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            72949..73149
                     /gene="rpmE"
                     /locus_tag="ACOIAS_000068"
     CDS             72949..73149
                     /gene="rpmE"
                     /locus_tag="ACOIAS_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGZ2699803.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            73294..74187
                     /locus_tag="ACOIAS_000069"
     CDS             73294..74187
                     /locus_tag="ACOIAS_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HGZ2699804.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            74205..75053
                     /gene="prmC"
                     /locus_tag="ACOIAS_000070"
     CDS             74205..75053
                     /gene="prmC"
                     /locus_tag="ACOIAS_000070"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGZ2699805.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            75097..76161
                     /gene="prfA"
                     /locus_tag="ACOIAS_000071"
     CDS             75097..76161
                     /gene="prfA"
                     /locus_tag="ACOIAS_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGZ2699806.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            76290..76970
                     /locus_tag="ACOIAS_000072"
     CDS             76290..76970
                     /locus_tag="ACOIAS_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HGZ2699807.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            76999..78039
                     /locus_tag="ACOIAS_000073"
     CDS             76999..78039
                     /locus_tag="ACOIAS_000073"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGZ2699808.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            78056..78505
                     /locus_tag="ACOIAS_000074"
     CDS             78056..78505
                     /locus_tag="ACOIAS_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HGZ2699809.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            78522..78974
                     /gene="rpiB"
                     /locus_tag="ACOIAS_000075"
     CDS             78522..78974
                     /gene="rpiB"
                     /locus_tag="ACOIAS_000075"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HGZ2699810.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            79010..79639
                     /gene="upp"
                     /locus_tag="ACOIAS_000076"
     CDS             79010..79639
                     /gene="upp"
                     /locus_tag="ACOIAS_000076"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGZ2699811.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            79940..80755
                     /gene="yqeB"
                     /locus_tag="ACOIAS_000077"
     CDS             79940..80755
                     /gene="yqeB"
                     /locus_tag="ACOIAS_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGZ2699812.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            80981..81418
                     /locus_tag="ACOIAS_000078"
     CDS             80981..81418
                     /locus_tag="ACOIAS_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGZ2699813.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            81603..81830
                     /locus_tag="ACOIAS_000079"
     CDS             81603..81830
                     /locus_tag="ACOIAS_000079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HGZ2699814.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            81835..82221
                     /locus_tag="ACOIAS_000080"
     CDS             81835..82221
                     /locus_tag="ACOIAS_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HGZ2699815.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            82224..82928
                     /gene="atpB"
                     /locus_tag="ACOIAS_000081"
     CDS             82224..82928
                     /gene="atpB"
                     /locus_tag="ACOIAS_000081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGZ2699816.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            82997..83257
                     /gene="atpE"
                     /locus_tag="ACOIAS_000082"
     CDS             82997..83257
                     /gene="atpE"
                     /locus_tag="ACOIAS_000082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HGZ2699817.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            83380..83877
                     /gene="atpF"
                     /locus_tag="ACOIAS_000083"
     CDS             83380..83877
                     /gene="atpF"
                     /locus_tag="ACOIAS_000083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGZ2699818.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLVEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            83874..84419
                     /locus_tag="ACOIAS_000084"
     CDS             83874..84419
                     /locus_tag="ACOIAS_000084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGZ2699819.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            84436..85938
                     /gene="atpA"
                     /locus_tag="ACOIAS_000085"
     CDS             84436..85938
                     /gene="atpA"
                     /locus_tag="ACOIAS_000085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGZ2699820.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            85966..86844
                     /gene="atpG"
                     /locus_tag="ACOIAS_000086"
     CDS             85966..86844
                     /gene="atpG"
                     /locus_tag="ACOIAS_000086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HGZ2699821.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            86857..88251
                     /gene="atpD"
                     /locus_tag="ACOIAS_000087"
     CDS             86857..88251
                     /gene="atpD"
                     /locus_tag="ACOIAS_000087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGZ2699822.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            88254..88514
                     /gene="atpC"
                     /locus_tag="ACOIAS_000088"
     CDS             88254..88514
                     /gene="atpC"
                     /locus_tag="ACOIAS_000088"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HGZ2699823.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            88976..89356
                     /gene="acpS"
                     /locus_tag="ACOIAS_000089"
     CDS             88976..89356
                     /gene="acpS"
                     /locus_tag="ACOIAS_000089"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGZ2699824.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            89371..89832
                     /locus_tag="ACOIAS_000090"
     CDS             89371..89832
                     /locus_tag="ACOIAS_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGZ2699825.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            89853..90443
                     /gene="gerS"
                     /locus_tag="ACOIAS_000091"
     CDS             89853..90443
                     /gene="gerS"
                     /locus_tag="ACOIAS_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="germination lipoprotein GerS"
                     /protein_id="HGZ2699826.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            90458..91615
                     /gene="alr"
                     /locus_tag="ACOIAS_000092"
     CDS             90458..91615
                     /gene="alr"
                     /locus_tag="ACOIAS_000092"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGZ2699827.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            91648..91935
                     /locus_tag="ACOIAS_000093"
     CDS             91648..91935
                     /locus_tag="ACOIAS_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HGZ2699828.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            91904..92287
                     /locus_tag="ACOIAS_000094"
     CDS             91904..92287
                     /locus_tag="ACOIAS_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGZ2699829.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            92458..93285
                     /locus_tag="ACOIAS_000095"
     CDS             92458..93285
                     /locus_tag="ACOIAS_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGZ2699830.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            93278..94219
                     /locus_tag="ACOIAS_000096"
     CDS             93278..94219
                     /locus_tag="ACOIAS_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGZ2699831.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            94642..95736
                     /locus_tag="ACOIAS_000097"
     CDS             94642..95736
                     /locus_tag="ACOIAS_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2699832.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            95962..96378
                     /locus_tag="ACOIAS_000098"
     CDS             95962..96378
                     /locus_tag="ACOIAS_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2699833.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(96560..97129)
                     /locus_tag="ACOIAS_000099"
     CDS             complement(96560..97129)
                     /locus_tag="ACOIAS_000099"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGZ2699834.1"
                     /translation="MKKEFRKKVIESRKNKNANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            97283..98440
                     /locus_tag="ACOIAS_000100"
     CDS             97283..98440
                     /locus_tag="ACOIAS_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HGZ2699835.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            99044..99526
                     /locus_tag="ACOIAS_000101"
     CDS             99044..99526
                     /locus_tag="ACOIAS_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HGZ2699836.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
     gene            complement(100225..101379)
                     /gene="nagA"
                     /locus_tag="ACOIAS_000102"
     CDS             complement(100225..101379)
                     /gene="nagA"
                     /locus_tag="ACOIAS_000102"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGZ2699837.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYIPALSCNIDNVCGSIHQGMEADFIVTDKDFNIYETYISGICKYKVNK"
     gene            complement(101462..102187)
                     /locus_tag="ACOIAS_000103"
     CDS             complement(101462..102187)
                     /locus_tag="ACOIAS_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2699838.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            102454..103383
                     /gene="pfkB"
                     /locus_tag="ACOIAS_000104"
     CDS             102454..103383
                     /gene="pfkB"
                     /locus_tag="ACOIAS_000104"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGZ2699839.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVSK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYYVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(103512..104429)
                     /locus_tag="ACOIAS_000105"
     CDS             complement(103512..104429)
                     /locus_tag="ACOIAS_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGZ2699840.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMQFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECIKAQRLLEKIINSVK"
BASE COUNT        41717 a        11458 c        18882 g        32625 t
ORIGIN      
        1 aaactagtat tactaggttc tataaactta ttaaaaactt aatagaaata ttaagcataa
       61 agattatgtg gttacaatag cagagaggat acacctgttc ccattccgaa cacagaagtt
      121 aagctctcta gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacgtagcca
      181 cgtaattttt tttatttttt gtcaaaaaaa acaaaaaata cgtatttctt ttttcatttc
      241 gggaaaacta aacacaggaa ttatggactt tataaaaatt taattcttga ggagtgatat
      301 aagtgaaaaa agttgcttta attttagcta taataggtgc gcttaactgg ggtgcaattg
      361 gtattctagg tacagactta ataggtagta tctttggtgg aacatatgaa atggtaagta
      421 gaataatata cttcattgta ggtttagctg gattatactt aataccaagt ttaatgtctg
      481 atgatagaga ataatttatg cttttatgtt aaatacctca ttctaaatgt ttagaatgag
      541 gtatttaatt ttataaaaat attaatttgt ttctttttta atggtaaaat agataagaat
      601 tgaagtttta aaaagaagta ttatgtaaaa aaggagtact attaatatat gtttattatt
      661 aatgaattaa ataagattgt aaatgaagaa gtaatatctt ttcatatgcc tggtcataaa
      721 aagggacagg tttataaaaa gttaggatat aaagatatac taaaaaattt gtataaaatg
      781 gatacaactg agatattagg aacagataat ttacactttc cggaaggaat tataaaaaat
      841 tcacaaaaaa aagcatcaag agtatttaaa agtgattata cttgctattt agtaaatggt
      901 agtagctgtg gaatacaatc tgcaataatg tcagtatgta gtcctaaaag taaaataata
      961 acaaatagag actgtcatca gtctgttata aatggatgta ttttaggaga tatagatata
     1021 gagtatgtgc cttgtgagat aagtgaagag acaaatatct taaaagggat atctataagt
     1081 aatgtgattg atataataga taaaaacttg gatgcaaaag cagtactgct tacttatcct
     1141 acatattatg gaatgacata tgatttagaa tacatatgta attatgcaca tagcaaaaag
     1201 atggtagtga tagtagatga agcacatggt gcgcatttag ggcttagtga gaggcttcct
     1261 aaaacagctc ttgagcaagg tgcagatata gttgtacaga gtacacataa aactcttcca
     1321 gcatttacac aatcgtcaat gatacatata cagggagacc gagttgatat agataaaata
     1381 ttgaacatgt taagaattac agaatcttca agcccttcat atttattatt atcttctctg
     1441 gaattagcag tagatatata tgaaaataaa ggaaaagaat taatggaaga gcttttaaat
     1501 aatattataa ctttcaaaaa taaagttgat tcaaatatta atatatacga cacaaatgat
     1561 aaaacaaaaa tatttatatc atctaaaaac atgggtttaa caggttatga actagaaaat
     1621 attttgagaa ataaatataa catacaagtg gagttatcta attattatgg agttctttta
     1681 atatgtacaa taggaaatac tgctgaggat ttctctagtt tagaaacagc attaaacaat
     1741 gttgtaaagg aagactttaa atttgaaaag ttagaaaata aaaaataccc tgttgttatt
     1801 ccaaagaaaa tattaacacc tagagaagca ttttataaaa ctaaaaaaag tgttaaaata
     1861 tatgacagta taggtaaagt ttgtggtgaa tgtatagttc catacccacc tggaataagt
     1921 gtaatatctc ctggtgaagt aataagtaaa gaaataatag actatctaat gttttgtcat
     1981 tctaaaggca tgattgtgag tggattgaaa gatgtagatt taaattttat acaagtaata
     2041 gatttagaag acatttaaaa attatgatat aaaagtagat agaagtagat agtttaaaat
     2101 tgtgaagaga aataaataga ctgcttatat agagatatct aataaaagta ctaaaaaagt
     2161 aaaaagtgtt tgtatggagg atatgaaaat gaaaggtaag ttaataatta tagagagtgg
     2221 ttcagatgca agtggaaagg ctactcagac agaaatatta tatgaaagat taaaaaaaga
     2281 aggctatatg attaaaaagg tagaatatcc aaattatgaa tctccatcat ctgccttggt
     2341 gaaaatgtac ctaaaaggag actttggtaa aaatgcatca gatgtagacc catatatagc
     2401 atcaactttt tttacagctg ataggtatgc atcatttaaa accgattggg aggagtttta
     2461 caatgaaggt ggaataatta tcgcagatag atatacaacc tctaatatgg tacatcaggc
     2521 ctctaaaatg gatatttgtg atagagaaga gtatttaaat tggttagctg attatgaatt
     2581 taatttattt aagataccac aacctgattg tgttatattt ttagatgtgc ctattgaatt
     2641 tagtaaaaag cttatggaga atagaaaaaa taaaattaca ggggaagaaa aaaaggatat
     2701 tcatgaaagt gacatagagt atttagagaa atcttacaac aatgctttat atatagcaga
     2761 taaatatgat tggaaaaaaa taaattgtgt tttagataac aaattaagaa gtattgaaag
     2821 tatacataat gaaatttata aaatagttct tgattcaata aaacctatgg agagataaat
     2881 atgtattttg aaaatattat agggcaagat tttgcaaaga aatatttaac aaattctata
     2941 aagaaaaata aacttaataa tgcgtatatg tttgaaggaa tggatggaat aggaaaaaag
     3001 aaatttgcag atgaattatc aaaactatta cttgattatg aaaatctcga aaatagtcca
     3061 gactatgtac tcataaaacc agatggaaat agtataaaaa tagctcaaat cagaaattta
     3121 caatcagata tagtaataag acctcataaa gactataaaa tatatataat caataatgct
     3181 gaaaaaatga ctgtggaagc tcaaaatgca cttttaaaga cgctagagga acctccaaac
     3241 tatgctataa taatcttagt tacaaacaac aaggaatctt tattagaaac tataaaatct
     3301 agatgtgata taataaagtt ctcacctata ccaattgaag acttaaaacg ttatttgata
     3361 aatacaggta tagaagaaga aagagctcaa ttattggcta ttttttcgag aggaagtatt
     3421 gagaatgctc taaatctatc tcagtcttct gagttttcaa tgatgagaga agatatacaa
     3481 cagtatattc agataatgtt agataaaaac atagtggaga ttttaaatat accaaataat
     3541 atggaaaaat atagaggtaa gattatagct ttattagata tgatgataaa ctattttcga
     3601 gatatcatcc tattaaaaga aaatgtaaac aagaacatgt taataaatgt agataagcta
     3661 gttttcattc aaaatatgaa tggaaaaatt agttattctc aattgtccaa gattattgat
     3721 ataatagaag atgcaaagag taaaataaaa agcaattgca atttcaatat aagcatacaa
     3781 gttatgtctt taaatatata cgaggtgatt aaatgataaa gatagtaggt ataaggttta
     3841 aaagtgctgg gaaaatatat tattttgacc ctgttgattt taatatagag caagatatgg
     3901 atgtagttgt agaaactgca agaggcttgg agtatgggaa agtagtcgtt ggtccaaaag
     3961 atatggatga aagcgaatta gtttctcctt tgaagccaat tataagaata gctacagaag
     4021 aagataaaaa agtatatcta gaaaataagg aaaaggcaaa agaaactttt gagttgtgcc
     4081 aacaaaaaat aaaagaacat gagttaacaa tgtttttaat agattgtgag tatacatttg
     4141 ataggaataa gttgattttt tactttacag cagaaggaag aatagatttt agagaattgg
     4201 taaaagactt agctgctata tttaaaacta gaatagaact tagacaaata ggtgtcagag
     4261 atgaagctaa atctatagga ggtctagggc cttgtggtag aaaactttgt tgttcatctt
     4321 ggttaggtga ttttcaacca gtttcaataa aaatggcaaa agaccaaagt ctatctctaa
     4381 atccaacaaa aatatctgga atatgtggga gattattctg ttgcttaaaa tatgagcatg
     4441 atgtatatag tgaagcaata gaaaaaatgc ctgttgtagg tgcattggtg caatcagatg
     4501 atggaaaagg aaaagttgta gaagtgaatc ctttattaga acaaattaaa gtggaatttc
     4561 aagacaagac agtaaagata tacacaagag aagatataaa aatacttaga gagcctaaaa
     4621 aatgtgatgg ctgtggtggt aaatgtaagg ataatgatgg attagatgaa gccacactaa
     4681 gagagctaaa gaaattagaa gattaaattt aaggtagggc ttttcctacc ttttttaaag
     4741 caattaatgc ttaaagttat taatatttga ggagaattta tatatgcaat taaaagattc
     4801 agaaagaata gatgatttac aattaaaagg ccttaaaatt atacaagata ctaatggttt
     4861 ttgttttggt atagatgcag ttttattagc caattttact aaaataaaaa aagatgccaa
     4921 agtagtagat ttgggtactg gaactggcat tatacctatt ttgatagctg gaaaaagtga
     4981 agctaaaaaa attattggcg tggaaataca agaagatgta tatgaaatgg caactcgttc
     5041 tataaaatta aatggattag aagaaagagt tgagattata aatgaagata taaaatacat
     5101 agacaaagtc ttagacataa atggttacca tgtagtcaca tcaaatcctc catatatgca
     5161 tattgatgga ataaaaaatc caaatgataa aaaagcaata tcaagacatg aagtaaggtg
     5221 caatttggaa gatgtaatta gagctgcatc cagattggtt atgcctagag gaaaattctt
     5281 catgatacat agaccaacaa gattggttga tataataact ttagggagaa aatataaatt
     5341 agaaccaaag gttattcaat ttgtgcatcc aagacctcaa aaagcaccga acttagtttt
     5401 ggttcaattt gtaaaagatg gaaggccaga gcttaaaata ttggacccat tgtatgttta
     5461 tggagaagat gggaattata caaaagaatt aaaagcaata tataataata aagatatagg
     5521 agagttgtaa atgagcggga aattgtacat atgtccaaca ccaataggaa acctagaaga
     5581 tataacatat agaactttaa gaatattaaa agaagtagac ttgattgcag cagaagatac
     5641 aagacatagt ataaaacttt taaatcattt tgaaatctca aaaccactaa ctagttatca
     5701 tgaacataat aaggattcaa agggagatta tttgataaat aaactaattg atggtgaaaa
     5761 tatagctctg ataagtgatg caggtatgcc tggtatatct gacccaggag aagagataat
     5821 aaaacaagct atacaaaata acatagagat agaagtattg ccaggtgcaa cagcctttgt
     5881 aacggcttta gttggctctg gtatggacac acatagattt atttttgagg gctttttaga
     5941 tagagataaa aaagttagga aagcacaatt ggaagaatta aaggaagaaa gtagaactat
     6001 aattttctat gaatcaccac atagattgaa ggatacacta aaagatatgc ttaaaattct
     6061 tggtaatagg aatatttcta taaatagaga acttacaaaa aaatatcaag aaataataag
     6121 agaaaatata gaaactacca taaaaatatt tgaggaaaaa gaagtaagag gagagtttgt
     6181 attaatagta gagggttttc atggtgagaa gtcaaagaaa gaaaattatg atgggcttac
     6241 agatagggaa tatgttttaa aactaattga aagtggtatg aacaaaaaag atgcaataaa
     6301 aatagtttgt aaagatagaa agttaaagaa agatgtggtt tataaacaag ttttagattt
     6361 ataaaacctg aatctgaggt gattttatgg aaatagggaa aattataata gagagtttaa
     6421 agagggaaga tacacataaa atatttatag aattagataa taagcatgaa ttagaaaaaa
     6481 taattcctaa aataagctgt atgaagtctg taggtgaatg taaatatcat gtagtaaatt
     6541 gttttgaaca ttctataaat gcattaaaag aattagaaat agtattaaat gataaggatt
     6601 tttttccaat acatctaaga aaacatgtgt caaactattt aaaaacatat attgaagaag
     6661 gaataaataa gttacatgta ttaaagctgg gaacattttt acatgatata ggtaagcctg
     6721 acagtatgac cttagatgaa accggaagag ttcatttcac aaatcatgaa aaaataggtg
     6781 ctcaaataat tgacaatatg ggaataaagt tagagttatc aacagagaca tatagactta
     6841 taagtaaata tgtaagatat catatgatac ttttgtctct atataagaaa aatgatttga
     6901 gtaggaaaga attaattaat gtatttaact tagtagatga ggacactata ggtgtaataa
     6961 tgcttgggta tgcagatata gtgtctacag taaaattatt gggcaagagt ggagaagtca
     7021 gtgtgttaaa gacatatatg gaatatattt taacaaatta cttgtataag agtaattata
     7081 cacatgtata aatttagtta atataattga gttttagatg attaattgaa gtttgaaata
     7141 tacggatttc aaaaacataa aagaagttgt ctttgttgta aaattatgaa agacaacttt
     7201 ttttatatca aaaattgaaa ttttattaag gatacttaat ggtctattaa ttaataaacc
     7261 attagtattt aatttatatt acaatattga ttttgcttgt attggcgttg atagtataac
     7321 agaggtctta gtagaaccaa ttttctttat agtatcaatc acattttcta gctcgtacat
     7381 atcttttaca attactttta aaagtaagca atcatcacct gttatatggt gacattctac
     7441 aatcctaggg tcctttgcag ctgactcaat aaattctgta tatccattgc ttggaagaga
     7501 aatatgaata aatgccttta taactctgcc taatgaatct gggttgacaa tagctttata
     7561 tccttctata acaccagact cttctaatct tttgactctt tctgaaactg caggagaagt
     7621 taaaccaact atttttccta agtccttcat agaaattctc ccatcatcct gtaaaatttc
     7681 tatgattctg taatctgtaa catccataaa taaacagtcc cccttaaata aaaataatta
     7741 atgctataaa acttttttag aatctatcat ttgtgttttt aaataccaaa gatttctaga
     7801 taaatcaacc ttataaatgt gtggttttga aagtcttttg tattgttccc agatagtact
     7861 taattcatat tgtacatgat tttttatttc aagaacagct tttttattat atttgcatct
     7921 gccttttata tacaatggtt taagaagctc tttaactttg taatttgtaa ataccttcgt
     7981 tttccatgta taagtaggat ggaagatttc taaaggttta gactcatcta taacttcatc
     8041 atgtaacata atcaaatctg cctctgcctt attgtcttcg ttatatattc ttataacttt
     8101 cttgtaacca ggattattta ttttttctgg attctcagaa atctttatct taggttcaag
     8161 aactcctttt tcacaagatg cagctaattt ataaactcct ccaagagaag gtgaatcgaa
     8221 agatgtaatg agctttgtac ctactcccca tgaatttata gtagctcctt ctgctttaag
     8281 tgatgttatg gtatattcgt ctaggtcatt agatgcagtt atgctgatat ctgaaaagcc
     8341 agcattatct aattgttttt tagcttcttt agataaataa gctaaatctc cagagtcaag
     8401 tcttataccc ataggtttgt atcctttttc agaaatattt ttgaatactt ttattgcatt
     8461 aggaactcca ctgtttaata catcataagt atctacaagt agtaggcatt tatctggata
     8521 aacatcagca tatgcttgga atgcttcaag ttcattatca aatttttgta cccaactgtg
     8581 agcttgtgta ccaattatag gtatatcaaa cattttccct gctagtacgt ttgcagttcc
     8641 agcacaacca ccaactacgg cagccctagc cccatatatc cctgcatctg gaccttgtgc
     8701 acgacgtaat ccaaattcaa atacagggtc tccttgagct gcaaagcaaa ctctagatgc
     8761 tttagtagca ataagagact gaaaattaac tattgttaac aatgctgttt caataagttg
     8821 agcttgatat aaaggtgcct taacagtaat taatggctca ttagggaaca tgatagttcc
     8881 ttcctcaaca gcatatatat ctcctgtgaa tttaaattct tttaaaaatt ttaagaactt
     8941 gtcagaaaat aaatttaagt tttttaagta ctctaaatct tcatctgaaa aatgaagatt
     9001 atctatgtat tcaacaacct gatcaatacc acaaactata gtataccctc catcacaagc
     9061 gttttttcta aaaaacatat caaatacaac aatgtcttca tgaatattct tttcaaaata
     9121 gccatttaac atagtaagct gatataaatc agtaagaagt gttagatttc tcattatttc
     9181 tctccttaat tattattaaa tattaaaaat tgtaaaaact cccacactta tactaaaaca
     9241 taatatagta caactttcaa gtataaagta ttacctaact atcataaaat tatttaaaga
     9301 ggaggatgat tataatagga aaaatatggt tttatatggt atcgatagga ataataggaa
     9361 gcttgatttc taaaaatatg ggtgaattaa ataaagttat attaagtgaa acatcaaaag
     9421 gtatagagtt tgcaataagt ctagctggta tcatggcact ctggatgggg ataatgaata
     9481 tcgcaaaaga atcaggttta attgaaaaga taggtcaaaa actaaatcct ataatgagaa
     9541 gattatttcc ttctattcca aaaggtcata aagcaatgtc ttatatagtt atgaatattg
     9601 ctttaaatat gttgggtgct ggaaatggtg caacagcatt tggtttaaaa gcaatgagtg
     9661 agcttcaaac tttgaataac aagaaagata cagccactaa tgatatgatt atgtttatgg
     9721 taatcaatat atcatctata caaataattc catttactat aatcaaacta aggatggata
     9781 tgggggctca aaatccaagt gaaataattt ttacgacttt atttgcaacc ataatatcta
     9841 cagcagtagc aataataaca tgtaaatttt ttcaaagaag atatagataa aggggatgta
     9901 gatacatgga gatattttca aacttactta taccaataat aattctgtac atagtagttt
     9961 atggtaaata taaaaaaata gatgtgtatg atagttttgt aaaaggagca attgatgggt
    10021 taaaagcagc atgggatatc ctaccatata taataggtat atttttggca ataggaatat
    10081 tcaaaactgg taaaggtctt gatatgttag aatggctatt tacaccaata gcaaatatga
    10141 tgagtatacc aaaagaatta ataggtctaa taagtgtaaa acctttatct ggaagtggtg
    10201 ctttagggat gtacagtgaa cttgcaaata gagttggaat aggaagttta gtagaaaaaa
    10261 tgggtgctac gattgtaggt tcttctgaga ctatttttta cacaatggca atatactatg
    10321 gaagtctgaa gataaaaaat actagacata cattatcatg tgctatgatt agccatgttg
    10381 caggtgtcat agcagctgtt tttatttgct atgtaatatt tgtataattt gttgacaata
    10441 tgggaaaaat tttataatat agtgattata tagaaacaaa tagtaataca aattacaagc
    10501 tatgaaaaga agagtaaata aaggtttttt ctatcagaga gctaggttag gtgaaagcta
    10561 gtgtcaaaag ctttattgaa gatggtcttg gagcttttta tctgagcaag tgtgctaggg
    10621 taaaacggtt ggacacgtta tagtctacta aggtatctat aaaatgtttc tacatttcat
    10681 gtgaaacata actattatag atgaattagg gtggtaccgt gaataatctc gcccctatgt
    10741 taactcatag gaggcgattt tttattattt aaatgaaaga atcatatgat tacgaacgat
    10801 aaaaaattag gaggtaaata acatgagcaa accgagtttt tatgtaacaa caccaatata
    10861 ctatccaagt ggaggattac acataggaca tacttattca acagtagctg cagatacaat
    10921 agctagattc aagcgtttct gtggatatga tgtaaagttc ttaacaggaa cagatgagca
    10981 tggtgaaaaa attcaaaaga aagccataga acaaggaatg tcagagatag agtatcttga
    11041 tggaatgata aaagatataa aggccttatg gaatactatg gatatatctt atgatgattt
    11101 tataagaact acagaaaaaa gacatacaga cataatacaa aagatattta ctaaactata
    11161 tgagcaaggt gatatatata aaggtgaata tgaaggtaga tactgtactc cttgtgagag
    11221 tttctggaca gaatctcaat tactagaagg aaataaatgc cctgactgtg gaagagaaac
    11281 atatttagta aaagaagaat cttatttctt tagattatct aaatatgaag acagattaaa
    11341 agagttattt aaagatgata gtttctgttt tcctgctgct agaaaaaatg aaatggtggc
    11401 aaacttttta gacaaaggat tagaagattt aagcgtaact agaacgactt ttgactgggg
    11461 tataaaagtt ccttttgatg aaaaacatgt aatttatgtg tgggtagatg ctttatgtaa
    11521 ctatataaca gcattaggtt atatgactga taatgatgaa gaatttaaaa aatattggcc
    11581 tgccaatgtt caaatagtag gtaaagaaat agtgagattc catacaataa tatggccagc
    11641 gcttttaatg gctttaggtc tagaagttcc aaaacaagta tttggacatg gatggatact
    11701 atttgctgat gataaaatga gtaaatcaaa aggtaatgtg gtttatccag aacctataat
    11761 agaaagatat ggaatagaca cacttaagta cttcttatta agagagtttg catttgggca
    11821 agatggaagt tatactcata gaaattttgt aacgagaata aattatgatt tagcaaatga
    11881 tttaggaaac ttaataagta gaactgtagc tatggttgaa aaatacaata atggtataat
    11941 accaacagca aaagtttcaa ctaattttga tgctgactta aaagaacaag ctgtttcaac
    12001 tagagaaaac tttgaagctg aaatggataa aatgcaattc catgaagcat tagagagtgt
    12061 atggaaatta gttagaagaa ctaacaaata tatagatgaa actatgcctt gggcactagc
    12121 taaagatgaa actaagaaag atgaattaga cacagtttta tataatttat gtgaatctat
    12181 aagaataatc gcaacactaa taaatcctat aatgaatgag actgctaata aaatatatga
    12241 acatatagga ataaaaggtc aagatgatat aactacatgg gaaagtacaa aaacatttgg
    12301 tcttattgga gaaaatgtaa aagtatttaa aggtgaacct ttattcccaa gattagatgt
    12361 agaaaaagag atagaagaat taactaaaat gttctctggg aaacctccag tagaagaaaa
    12421 acctttagag cataaagaag aaattactat tgatgattta gacaaaatag agcttagagt
    12481 agggaaaata ataagctgtg aaaaacatcc aaaagcgaat aaattattag tatcacaagt
    12541 taagattgga ccagaaacaa gacaaatagt atcaggaata gctgaatact ataagccaga
    12601 ggatttagta ggaaaagagg ttacagtggt atgtaactta aaaccagtta aattaagagg
    12661 tgtagaatct caaggtatga tattagcagc aggtgatgat ggagacccat atgttttacc
    12721 atttacacaa ggggctaaag atggatgtga ggttcgttag taggtaatta aatagtaagg
    12781 agattaaaga tgttatttga ctcacatgca catttaaatg atgaaagttt tgatgaagat
    12841 agagatgaac ttattggttc attaaaagat aaaggtgtag atttggttgt aaatccagga
    12901 gcagatatag agacgtcgat aacagcaatt gaacttgcga aaaaatacga ctttatatat
    12961 tcagcagttg gggtacatcc acatgatgta tcaaaactag atgatacagc aatagaaact
    13021 ctaagaaaac ttgcaactga gaatgaaaaa gttgtagcta ttggagagat tggattagat
    13081 tattattatg attattctcc aagagaagaa caaaaagagt ggtttaagaa gcaaattgaa
    13141 ttagctaatg agctaaaact tccaataata atacatgata gagatgcaca tggagatact
    13201 tttgagataa taaagaatac taaaaatcct gaaataggat gtgtacttca ttgttatagt
    13261 gggaatgtag agttagctag agaatatgtt aagatgggtt gttatatatc aattccaggt
    13321 acggttactt ttaagaataa taaaaaaact agagaagtag taagagaaat acctctagaa
    13381 agattattca tagagactga ttcaccatat atgtcaccag agccacatag aggaaagaga
    13441 aacaatcctt ctcaagtatc ttttgttgct gataagatag cccaggaaaa agggatatct
    13501 tatgaagaag tatgtagagt tacaaaagaa aatgcaaaga aattttttaa tataaaatag
    13561 acaaattaaa tcttataatg atatatataa taagtcatta taagtatagg atgagagtgc
    13621 ctcaaaatga gttttttagt ttatgaggca ctctttttgt atgaaatcaa atatattttc
    13681 atcatttcaa caatgaaaaa gaagtttatc atattttgag atagtttgtt tttatttatt
    13741 taattcatat tttaaaatat attttcttaa cattgttaaa aaagaattta caagatattc
    13801 attagaatta actcaactct aatattcact taacttaagt tgtttattat attattaaag
    13861 agaagtaaac gaaaagtaga ttaaggaggt tgaattttgt atacagttga tagtactaag
    13921 atattgataa atatgaactt agagaaacta gaaaagatat actcaatgat aaaagataca
    13981 tcaagtattt ctataattaa agaacctaac ttggctacag taatggtaag agctaatgaa
    14041 agtgtaaaaa acacaacatt taatttagga gagatattag ttactgaatg tagtgtaaag
    14101 gtagatgaaa gcttgggtta cggaatagtt tcagaaaaca ataataagaa agccatttat
    14161 ttagcagtta tagatgcagt tttacatagc aataacagta aatttgatga gttaaagaat
    14221 tacataaata aaagtgtata tgaagagaac ttaaggtatg aacaagagat tattgatgaa
    14281 tttagcttga ttaataaaac gaaagttcaa ttcaatgcta tggattaaat aggaggagaa
    14341 tcatggtaaa ttatacacaa gaaatatata gaaagatgct agatagtgct tcatttcctg
    14401 ggaaattaaa taatatagat aatataaata tagaaaataa tacaaaacta ttgaatggag
    14461 caattggtat agcaatcact ttattagacc aagaagttac cttctatata gaaaattata
    14521 ctcaaaaaga tataaaaaat ataagagcac ttacaatgtc aaatcaaata gattatagaa
    14581 aatcagatta tatattttta gatattaatt cagaagtgga tatattacag attaaaatag
    14641 gttcttttga atatcctgat gaaagtgcaa ctatagtaca tcaagtaaat gatttatcaa
    14701 cgtactctca aactaagtat ataaagcttg atttgagtgg tcctggtata aaatctaaaa
    14761 atagtatata tataaatgga attagagaag aatttataga aagattatct agcataaata
    14821 aagattatcc aataggaata gatttgattt tagtagataa aaaaggagaa attgcattta
    14881 taccaaggtc atcaaaatta agttgggagg tattatagaa atggcttatg tagcagttaa
    14941 aggtggagaa gaagccatat tacaggctaa aaatatactt gagttttata gggtaaaagg
    15001 agatagcttg cctatagatg taaagcagat tgaggaacaa atgtcttatg ctgtagacaa
    15061 agtaatgtct gaaggaagtt tatacaacaa ggaactatca gcattggcta taaaacaagc
    15121 tcaaggtgat atattagaag cctctttcta tgtaagagct ttaaggtcaa caatgcctag
    15181 aattacatat tcagaaccca taaatacaga agaaatgaga ataaaaagaa gaatatcagc
    15241 tacttttaaa gatataccag gaggtcagta tcttggggca actagagact atgaaaatag
    15301 aatattgaat gtagaattat tagaagaaga tgaaaaaaag aatagacatt ttagagatag
    15361 ttttataaat gaattagaaa cagtagatga aatccctgaa tttacaaaaa tatctaatat
    15421 atttagaaaa gaaaatttat tacaagaaga agtttgtgaa aacaaattgg aagaagttat
    15481 tgatgatatt acaagaaata gtttaagatt tccagtttct aggtcagcaa agttacaggc
    15541 attaactaga ggtgaaagtg gagtagtgtc aggttttgct tatgctattg taagaggttt
    15601 tggtgatgaa catccatata taagtgaact tagaactggt aatgtattta taaaagtaaa
    15661 acatcccatc tatgaagatg aagaaataat aataggggat atgctggtta ctgaatgtga
    15721 aatagtttca aaagcatcac aaaataagac tgaaaattct aaaactaatt cggaagataa
    15781 aaataagatg tctttaggat atggtctttg tatggggaat aatgagaata aggctatatc
    15841 tatggccata ctagacaaat ctttggagcc aaatagtgaa aaactagcac aagatgaaga
    15901 atttgtattg cttcatatag atggaataga ttcattagga tttatatctc actttaagct
    15961 tcctcactat gtagatttta aagcggatgt agaaagaata aaatagtttg aggaggtaag
    16021 tttaggtgat atataactat ggttttttag acgaaaacac aaaaaaagaa ataagaagaa
    16081 agatattaaa agcaatatca atacctggtt atcaagttcc ctttggttca agagagttgc
    16141 ctatagctaa gggatggggt actggaggtt tacaattaac actatcatta attggtaaaa
    16201 atgatgtagt aaaagtaatt gaccaaggaa gtgatgattc tacaaatgct tgtaatataa
    16261 ggagctttat tgcaagtgta agtaatgtag aaaccacaaa agatacatta gaagctacat
    16321 tgattcaaac gagacataga ataccagaag aaaaacttaa atctaatcaa atactaatat
    16381 ttcaagtacc aataccagaa acccttagaa tagtagaacc tagtgaagta gaaacaagaa
    16441 gaatgcattc agaagaagat tatagtagga tgtgggtata tctttatgaa gatatagtta
    16501 gatttaatga tatatctata gcagttgaat atccatgtaa agtaaatgat agatatttaa
    16561 tgaatccatc acctatacct agatttgata taaaaaaatt aaatatgtca gacaatttat
    16621 tcttgtttgg tgcaggtaga gaaaaaagaa tttatgctat tcctccatat acaaaggtag
    16681 aacctttaga gtttgaggat tataagtttg aagaggaaaa gtttgaagga aaatactgta
    16741 gtttatgtaa cagtacaaat acatttttag atgaagtata tgacagtgac actaatgaaa
    16801 agtactatag ttgctcagat acaagttatt gtgaaaaagt tagattaaaa aataacagta
    16861 tagatgtgac aataggaggt acttggaatg agtaaaataa atgactctaa aagattagaa
    16921 aatatagaca agcaagtatt aataggaaga aatatcaatc ttatttatgg agatgggtgt
    16981 gaaaagtgtt ttgaaagcac aggatatgaa agcaataata tatgtaagta ttgtggttct
    17041 atagttgcat gtaataatgt aaatttagaa ctttatgaag gagaagtcct tggaattgta
    17101 ggtgaatctg gttcaggaaa aagcacttta ttaaaaatac tgtttttcca agaacatgca
    17161 aatagtggag aagtatatat atcccattac aataaagaca caaatatact agagctttca
    17221 gaccaaaaaa agagatacat aaagaatcat tttatgggta ttgtgtatca aaatccccac
    17281 cttggtttaa atttagactt tagtagtggt ggtaatatag cagaaaaatt attgatggca
    17341 aatttatata atgttgaaaa aataagagat agaggcaaag aacttttaat aaaaactaat
    17401 atacctgtag aaagaataga ccataaacca aaatacttta gtgggggaat gcaacaacga
    17461 gttcaaatag caaaagctct ttcaaataac cctccaatat tgctattaga tgaggttaca
    17521 acaggtttag atgtttctgt gcaagcggaa gtattggatt taataagaga aatccaaaga
    17581 gaacttaaga tttctatgat agttgtatcc catgactttg atgttataaa gatgcttgct
    17641 gataggacaa ttgtaatgaa aaatggaagt gtaattgagt ctggtcttac agaccaaata
    17701 atggaggacc cacaacaccc atatactcag caacttataa attcattgct ataaatatcg
    17761 tttgtaaata agcaaaatgg gggtttttaa taaaatgaaa gaaactatac ttgaagtaaa
    17821 taattttagt aaagactttg agttacatgc attgaacaag actataacgg catgtagtaa
    17881 tataagtttt gaagtttcaa aaggtgagtt tttaggaata ataggtaaat ctggagcagg
    17941 taagtctacc atattaaaaa gtatctataa aacatatata ccaacaactg gagaaataat
    18001 ttttaattca gaaatatatg gtaatgtaga cctggctaaa attggagacc gagaaatgat
    18061 aaatcttaga aagaaagaaa taggatatgt atcacagttt ttaaagacac ttccacgaat
    18121 aactgcaatt gaactggtag tacattcttt aattgagagt gggtttgaaa aagattgttc
    18181 gtatgatatg gcaaaagata tacttaacca atttgaaata aaggaaaatt tatgggatgc
    18241 gtatccaaat aatttttctg gtggagaaaa attaagatta aatttagctc aagctatggt
    18301 taaaaagcct agacttttgt tacttgatga accaactgca tcacttgata atcaatctaa
    18361 aatatatgta aaagaaaaaa tgttggagct taaaagtcaa ggaactacaa tgataggaat
    18421 ttttcatgat atagagttta tggaaactgt tatagataag acctttacta tgacaaaagg
    18481 gactataagt gaaagtgggg tagcctagat gataaaacta attcataatg caaatattgt
    18541 attagaagat atgattttag aaaatggtca tttaatcata gagggagata caataaaaaa
    18601 agtatcaaat gataagattg attacgcatt tgactttaat gagatagatg aaataataga
    18661 tgcaaaaaac ttatatgtaa tacctggtat tatagatatt cacagtgatg ccattgaaaa
    18721 ggaaatagag ccgagaccaa gtacattatt accatttaat atggcatttt atgaactaga
    18781 taaaaaacta cctgcaaatg gaataactac agtttatcat tctatatctt taggggatgg
    18841 agtaggagtt agaagtattg ataactcttt aaagatgatt aaaaatattg attcatataa
    18901 aaatatagat agtaagagca taaatcataa agttcattta agatatgaag tgttgtatta
    18961 tgaaggttta gaaaaggttt tagaactatt agatgaaaat aaaatagact acttatcaat
    19021 aatggaccat tctccaggac aaggtcagta tacaaatcca acattttata aagagtatgc
    19081 tactaaagtg tggggagtta cagaaaatta tgtagataca tggttagatg atttagtaaa
    19141 tctgcatgat aatttagatt ggaataaaat agctaatatt ataggtatag ccaaaactaa
    19201 aaatataaat gttgcatcac atgatgatga tacattagaa aaaatggatt tcataaaaaa
    19261 tataggcata aatgtatctg aatttcctat aactcttgaa gttgcaaaac attcaaagaa
    19321 attgggtgtt ctaacttgtt taggtgcacc gaatattgta agaggtaaat ctcataataa
    19381 caatttaaaa gcaatggatg ctatattgga agattgttgt gacattgtgt gttcggatta
    19441 tcttccatca gctatgataa agtctatgtg tatagttgca gaaagaataa ataatttaaa
    19501 taaagcagtc agtttattta cctcaaatcc tgctaaagca gttggtatat atgatgaaag
    19561 aggtagtata aaagaaaata aaaaagcaga tttagtttta gtagatattg atagtgaata
    19621 tccaaaggtt gtaaatacta ttgtaaatgg aaaaacagtt tataaaaggg aggtataaag
    19681 attgctaaca gagttacatt gtcatacaag tatatcagat aataatttta ctactgaaga
    19741 aataataaaa aaagcagcag aaaagaatat aaatatctta gcaataacaa atcatgacac
    19801 attatctgga ttggatgaag ctcttgtgct tggagagcat tatggaataa caataatacc
    19861 aggtatagaa atttcagcct atgattataa aaatagaaaa agagttcata ttcttggata
    19921 taacatagat ttaaacagtc aagaaataaa aaatttgtgc aatccaatgg tatgtgacag
    19981 acataaagca tctgttaaga tggtaaataa aattatagat ttaggataca aaatatctct
    20041 ggaagatgta aagaaatatt catcaaagac tggaatattt aaacaacata taatgaaagc
    20101 tcttatagat aaaggatata cagataatat atattcaaat ctatataaag aattgtttca
    20161 tagaggaaac ggcaaggtat atgtatcttt aaaatatgtg gattacagag atgcaataaa
    20221 agctataaaa aattctggtg gaatttgtgt gttagctcac ccgggtcaaa tggataattt
    20281 tagcgctatt gaagagatgg taaaagtagg attagatgga atagaagtat atcatccatc
    20341 tcacaacaaa gaactagaaa aagagtcgct ttcttatgct aagaagtata atctagtggt
    20401 cacaggaggt tcagattacc acggatttta ttctcaacat aattatgaac ttggaagcaa
    20461 gagcttaaat aaagaagatt tatttaagtt taggttagct ttaggagggg tattataaat
    20521 gtctttttta caagtaaaaa atgtaggtaa aagttatgga caagttaagg tgttaaaaga
    20581 catatctatt gatattgaaa aaggagagtt tatttgttta ctaggtccaa gtggttgtgg
    20641 aaagagtaca ctacttagaa taattgcagg gcttgaggat aaacatggtg gaaaaataat
    20701 aataaatgat aaggatatga ctaattcacc acctgaaagt agaaattttg gtatagtttt
    20761 tcaatcatat gcattatttc caaatatgaa tgtttacaaa aacatagcct ttggactaga
    20821 aaataaaaac ataagtaaat ctaatattga taaaaaagtt aaagaagttc tagaagttgt
    20881 agaattaagt ggatatgaga aaaaatatcc atcacaatta tcaggtggac aacaacaaag
    20941 ggttgcatta gctcgtgcga ttgctttaga gccagacttt ttacttcttg atgaaccttt
    21001 atctgcatta gatgcaaaag taagacttaa acttagggag cagattagaa gtttacatag
    21061 aaaactaggc attacaacta taatggtaac gcatgaccaa gaagaagctc tttgtttagc
    21121 tgataagatg gttgttatga atagaggaga aataatacaa gttggaacgc caaaggaggt
    21181 atataaaaat ccagaaacac cttttgtagc tgattttata ggaacaataa attttataga
    21241 tgatggtatt aataagatag ctattagacc agaagatata aaagtagagt ctaatagaga
    21301 ttctaaagat aaggatataa aagtgggaga aattttagat atcgaattta gaggatttaa
    21361 ctatagaatt acagttgagt atcgttctaa gcaaatgaaa ttagatgtag tttccaaagt
    21421 tgcagaacaa atgaaactgt gcataggttc taaaataaac tttaagatac ctaaagaagg
    21481 aattgtacaa tataaatcag agggatgtgc ttaatagcta ggggtgaata aatgataaca
    21541 tcaaaaaata aaagaagaat aagtgatagc acaatacaaa aatatttctt gattttttcg
    21601 gtaattgtac taatagtatt tatattattt ccattagtat cattattaaa aaatgcattt
    21661 atggatacaa gtggaaattt tataggaatt tcaaacttta gtaaatatgt tgaaaatcca
    21721 agtcttatag catcatttaa aaattcaatt tttgtatcta cagtctcatc tttaatatca
    21781 ctatgtttag catttatata ttcatacgca ataacaagaa caaacataaa gtttaaagat
    21841 ttttttaaaa ctatgggaat gtttccactt tttgcaccaa ctatgttata tggtatttca
    21901 cttatatacc tttttggaaa taagggaatc tttacaacca tgggtctgga agtaccacta
    21961 tatggtccat taggaataat tatttctcag attctattta catttccaca agctttttta
    22021 atattatctg tagcactttc gatggctgat tatagacttt atgaagcagc agatagcttg
    22081 ggtgctagtg aatttaagaa attttgcaaa ataacaattc ctggtataaa gtactcatta
    22141 cttagttcat tctttgtatc ctttatactt gcatttacag attttggagc agctaaggta
    22201 gtcggaggaa actacaatgt actggctact gacatatata aacaagtagt tggacaattt
    22261 aacataccaa tgggagctac tgtttctatg gtaatgttaa ttcctgtact aatagcattt
    22321 acattagata agatggcaag taagaaacaa ggtatgacca taactgcaaa gtctatgcca
    22381 tataagatta aggaaaataa gtttagagat agagtatttt taatagtatg tagttttata
    22441 acattgctaa tttttatttt aatattcgtt tcagttatag gttcagttat aaagttatgg
    22501 ccatataatt taagctttac aatggaacac tatacattta aaggtatttt gggaagtgga
    22561 ataaaaacat acttaagcag tttgaaaata tcaactttaa cagcaatatt tggaactata
    22621 tttgtatttt ttagtgctta tatgatagaa aaggtggata agtttcctaa attaagacaa
    22681 acagcttacc ttttatcaat gataccaatg gccttacctg gcttagtttt aggtatatca
    22741 tatataactt tttttaactc agcaagtaat ccattaaatt ttttatatgg aagtacagca
    22801 atattagtta tagtaaatgt agttcatttt tattcagttt gttttataac ctcaaattca
    22861 tctttaaaaa tgttggataa agagtatgaa ttagttgcaa aatccataaa tattccattc
    22921 tataaagtat tttttaatat aacagttcca atgtctataa catcaatact tgaaatagta
    22981 gtatattatt ttgtaaattc aatggttact gtttcagcat taatattttt atatacacct
    23041 caaactcaga ctgcatcaat atcaatacta aagttagatg agataggata tataggaaca
    23101 tcagctgcta tggcagtatt agtgctttta acaaatataa tagtaagact tttgtacgaa
    23161 tttgtaacaa agaaattaaa aaataacaca caaaaatggc agcaaaaaga tgttgcatag
    23221 gggaggtaaa aatgaaattt aagaagctag tttctttagg agtattatca acacttttat
    23281 taagtgtaat aacaggatgt agtactgatt catcaggttc agattcaaag tctgatagta
    23341 agacaaaagg ggatttaaca gtttacacag caattgaaga agatagtata gagccatatt
    23401 tagctacatt taaagagaaa tatccagata ttaaattaaa tattgttagg gcatcaacag
    23461 gagatataac agcaagatta ttagcagaaa aggataatcc tcaagcagat gttgtatggg
    23521 gagttgcagc cacaagctta ttggttgcag atgaccaggg gatgttagaa ccatatgctc
    23581 caaaaggttc tgaagaaata gagtctgcat ttaaagatga taaagaagtt ccttcttggg
    23641 ttgggataga tgcttggatg acaggtataa cagtaaacac taaagaatta agtgataaga
    23701 acatcccagt tcctagttct tacgaagatt taataaagcc agaatataaa ggtcttatat
    23761 ctatgcctaa tccatcttct tctggtacag ggtacttaac agtatcagcc cttatacaaa
    23821 ttatgggtga agaaaaagca tggcagtata tggataaatt gcatgagaat attggtgtat
    23881 acactcaatc tggttcagct ccagctacat cagcagcttc aggagaatat cctattggta
    23941 tatcttttgg atatagaggt ataaagttaa aagaggaagg ctatccagta gaagtagtat
    24001 ttccaaaaga aggttctgga tgggatatag aagcaaatgc tcttgtgaaa aaagataata
    24061 taaaagaagc atcaaaagta tttttagatt gggctataag taaagatgta atgaacgaat
    24121 actctaagaa ctatgcagtt actactattt ctacaggaaa tccaatacca gaaggattcc
    24181 ctaaaaagcc attagagcaa atgatagata atgatttaaa atcagcagca aaaaatagag
    24241 aagatatact aaataaatgg atatcaaaat atgatggtaa gacagaaaaa gagagttaag
    24301 taaaactata caaatggagt aaaaagaaag aattttttta agtagatttt attataataa
    24361 tataagttga gaataatata ggtcaagaaa agtgaatatt ctataaaaat gtatagagta
    24421 agtataccat tcagtttgaa tggtatattt tttattttta gagattaatt tagaaataga
    24481 tatactacag tttaaaatag tatattttaa tttcattatt aaagtataga tattatatat
    24541 aaagtaaata aattattgag cgattattaa taagatatgt ataaatttta atttgggaac
    24601 ttgtgttata tatcatgaaa aaagagaaaa aataaaaggt aaattgtttc ttgataactt
    24661 gaaattggaa gtgagaatat ttaactagag aagaattatt taagtttaag tcagctttag
    24721 gaggaggaat tataaatgtc ttttttacaa gtaaaaaatg taggtaaaag ttatggacaa
    24781 gttaaggtgc taaaagacat atctattgat attgaaaaag gggagtttat ttgcttatta
    24841 ggtccaagtg gttgtggtaa aagtacattg cttagaataa ttgcaggact tgaggataaa
    24901 catgatggaa aaataataat aaatgataag gatatgacta actcaccacc tgaaagtaga
    24961 aactttggta ttgttttcca atcatatgca ctatttccaa atatgaatgt atataaaaat
    25021 atagcctttg gattagaaaa taaaaatata agtaaatctg atatagataa gaaggttaga
    25081 gatattttag aagttgtaga attaagtgga tatgataaaa aatatccatc acaattatca
    25141 ggtggacaac aacaaagggt tgcactagct cgtgcgattg ctttagagcc agactttttg
    25201 cttcttgatg aacctttatc tgcattagat gcaaaagtaa gacttaaact tagggaacag
    25261 attaaaagtt tacatagaaa actaggcatc acaactataa tggtaacaca tgaccaagag
    25321 gaagctcttt gtttagctga taaaatggtt gttatgaata atggagaggt aatacagatt
    25381 ggaactccta aagaagtcta taaaagtcca aaaacacctt ttgtagctga ttttatagga
    25441 acaataaatt ttatagatga tgatgtgaat atgatagcaa ttagaccaga agatataaag
    25501 gtagaattta atagagactt taaagaaatt gatgtaaaag taggagagat tttggatata
    25561 gaatttagag gattcaacta tagaattaca gttgaatatt gttctaagca aataaagttg
    25621 gacgtagttt caaaggtagt ggaacaaatg aaactatgtg taggttctaa aataaacttc
    25681 aagataccta aagaaagaac catacaatat aaatcagagg gatgtgctta atagctagag
    25741 gtggataaat gataacaata aaaaataaaa gaagaataag tgatagtaag atacaaaaat
    25801 gttttttagt ttttttagtg attgcattag tagtatttat attatttcca ttaatatcac
    25861 tattaaaaaa tgcatttatg gatacaagtg gaagttttgt aggaatttca aactttagta
    25921 aatatattga aaatcccact cttatagcat catttaaaaa ctcaattttt gtatctacaa
    25981 tttcatcctt aatatcatta tgtttagcgt ttatatattc atatgcaata acaagaacga
    26041 atataaggtt taaaaacttt tttaaaacta tgggcatatt tccgcttttc gcaccagcta
    26101 tgctatatgg tatttcactt atataccttt ttggaaacaa gggaatcttt acaattatag
    26161 gcatagaagt accactatat ggtccattag gaataattat ttgtcaaata ctgtttacat
    26221 ttccacaggc ctttttaata ttatctgtag cactttcgat ggctgattat agactttatg
    26281 aagcagcaga tagcttaggg gctagtgaat ttaagaagtt cttcaaaata acaattcctg
    26341 gtataaagta ttcattagtt agttcgttct ttgtatcttt tatacttgca tttacagatt
    26401 ttggagcagc aaagatagtt ggaggaaact acaatgtatt ggctactgac atatataaac
    26461 aagtggtagg tcaatttaac ataccaatgg gagctactgt ctctatggta atgttaattc
    26521 ctgtactaat agcatttaca gtagataaga tagcaagtaa aaagcaaggg atgactataa
    26581 ctgcaaagtc tatgccatat aagattaagg aaaataagtt tagagataga gtatttttaa
    26641 tagcatgtag ttttataact ttattaatct ttattttgat atttgtttca gttataggct
    26701 cagttataaa attatggcca tatgatttaa gctttacaat ggaacactat acgtttaaag
    26761 gtatatcagg aaatggaatc aaaacatatt taagcagttt aaaaatatca attttaacag
    26821 caatatttgg aactatattt gtatttttta gtgcttatat gatagaaaaa gtggataagt
    26881 ttcctagact gagacaaata tcttattttt tatcaatgat accaatggca ttacctggtt
    26941 tggctttagg tatatcgtat ataacttttt tcaattcagc aagtaatcca ttaaattttt
    27001 tatatggaag tacaacaata atggttatag ccaatatagt tcatttttat tcagtttgct
    27061 ttataacttc aaattcatct ttaaaaatgt tggatagaga gtatgaatta gttgcaaaat
    27121 ctataaatat tccattttat aaggtgtttt tttatgtaac acttccaatg tccataacat
    27181 caatacttga agtggctgta tattacttcg taaattcaat ggttactgtt tcagcattaa
    27241 tatttttata tatacctcaa actcaaactg catcaatatc gatactaaag ttagaagaga
    27301 tagggtatat agggccatca gctgctatgg cagtattagt gtttttaaca aatataatag
    27361 taaaactttt gtatgaattg acaacaaaga aattaaaaac taagacacaa aaatggcaac
    27421 aaaaagaagt tgcatagagg aggtaaaaat gaaatttagg aaattagtct ctttaggaat
    27481 attatcaaca cttttattga gtgtaataac tggatgtaat aatgcttcat caggttcaaa
    27541 tttaaagtct aataataaga taaaaggaga gttaacagtt tatacagcaa ttgaagaaga
    27601 tagtatagat acatatttaa ctacatttaa agagaaatat ccagatatta aattgaatgt
    27661 acttagatta tcagtaggag atataacagc aagattatta gcagaaaaag ataatcctaa
    27721 agcagatgtg ttatgggggg ttgcagctac aagtttgata attttagatg acaaagaaat
    27781 gctagagcca tacgctccga aaggctttga taaggtaaat cctgtattta aagatgataa
    27841 gaaagttcct tcttgggttg gaatagatgc ttggatgact ggtataacag taaatactaa
    27901 agaattaagt gataagaaca taccagttcc tagttcttat gaagatttaa taaagccaga
    27961 atataaaggt cttatatcta tgcctaatcc agcttcttct ggaacaggat atttaacagt
    28021 atcagaactt atacaaatta tgggtgaaga aaaagcatgg cagtatatgg ataaattgca
    28081 taaaaatatt ggtgtataca ctcagtctgg ttcagcgcca gctatattag cagcttcagg
    28141 agaatatccc attggtatat cttttggaaa tagaggtata aagttaaaag gtgaaggtta
    28201 tccagtagaa gtagtatttc caaaagaagg ttctggatgg gagatagaat caaatgcact
    28261 tgtgaaaaaa gataatataa aagaagcatc aaaagtattt ttagattggg ctataagtaa
    28321 agatgcaatg aatgagtatt ctaagaattt tgcagttact acaattgaca cgggaaatcc
    28381 ggtgccagaa ggatttccaa agaatccatt agaacagatg atagataatg atttaaaatc
    28441 agcagcaaga aatagagagg ctatactaaa taaatggata gaaaaatatg atggaaagac
    28501 agaaaaagag agctaagcct tgcaataaaa tttagtacaa aaagcgccta gaatataagt
    28561 tttatatcta ggctcttttg atatatttat ttaaaatgca tatttctata taaaaataaa
    28621 tagtttagta ccttgattaa attaaataaa aaccttaaaa taaagagatg taaagaaaaa
    28681 ctaaaaacga ataaaaggtg aatttatatg ataaaagaaa taatagtagt agagggtaga
    28741 gatgacgtta cagcagttaa gagagcaata gatgcagaac ttataactac aggtggattt
    28801 ggatttccaa aaggtgtaat ggagcgtata aaagctgcta atgaaagacg tggtgttata
    28861 atatttacag acccagattt tgcaggtgaa aaaataagaa aaaaaatagc atcagaagtt
    28921 ccaggatgta aacatgcatt tctaccgaga gaggaagcta aaaaggatgg agatataggt
    28981 attgagaatg ctactcctca aagtataata gcagctctga ataaagttag aactgaaaat
    29041 acagaaaaaa gagatgaatt taagcaagtt gacttaatta gaaatggtct tataggtaat
    29101 gaagaagcat cacttagaag agatgcactt ggaaaaatac ttggaattgg atatggaaat
    29161 gcaaaacaat ttttaaatag attgaataat tatggagttg aaagagaaga atttatagat
    29221 gcattaaaac aaatctaaac gtaatgttta atgtatgaaa aatatgtata taaaataaat
    29281 tgtggaatta cggttaattt aagatgaatt atttataata tacaccttat tttaaatcaa
    29341 tgtaaataca tataaataag tataattaat aaaattaaat atcaaaataa gttaaaaagg
    29401 agtaaataat ggatagacta tcttcacata atgcaacaaa ggaagtagta caaaaatata
    29461 attttaaatt ttcaaagtct ttaggtcaaa atttcttgat agatagtaat ataattgata
    29521 aaatcttatc tggagctaga atcacaagag gagataatat catagaagta ggacctggta
    29581 taggaactct tactcgtgag atgggtaaaa tagcagaaaa agtagtggct atagaaatcg
    29641 atagaaattt aattcctata ttgaaagata ccttgtcaga tttagataat acagaggttg
    29701 taaatcaaga tatactaaaa gtagacatac aggaattagt aaaagataaa ttaaatggtg
    29761 ggccagttaa gcttgtagca aatcttccat attatataac tactccaata gttatgaaat
    29821 ttttagagga agacatacct gtaacagaca tagtagtcat ggttcaaaaa gaagttgcag
    29881 atagaatgaa tgctattccg ggtactaaag attatggggc attatctata gctgttcaat
    29941 actattgtga tacagagatt gtggcaaaag ctccaagaca tatgtttata ccacaaccaa
    30001 atgtagattc cacagttata ggtctacatg taagagataa aagaaaatat gatgttcata
    30061 atgaagacat attttttaaa acagtaaaag catcctttgg acaaagaaga aaaactcttc
    30121 ttaattcgtt gggaggtctt ggttttctta ataaagatga aataagagaa gtattaaaag
    30181 aagctaatat agacgaaaaa agaaggggtg aaactcttag tatagaagag ttttcagttc
    30241 tttcaaatat aataaataca aaagtatctt ctaaatagaa ggtacttttt ttaattttgt
    30301 taacatatat tgtttagaga aactgtgtta aggggggatt gcagtgggtt ctaaaagaaa
    30361 gtttaaaaga gactatataa tactagaagc taaagacata aactttagat acaaagaacg
    30421 agtactgcca aaagcttttg caaaagttga agtaaatgat gaaaaagcaa ttattgcatt
    30481 atatgtagaa aatctaaaat atgttaaaga tgggtataaa gcagtagcaa taaaagatga
    30541 ccatggatta atagacttag gaaatataat tttaaatgaa caaggaagag gtgaatttgt
    30601 tttagattta gaagattatg atgatgagat aaaaggtatt gcattattgt atgaaagaaa
    30661 tataccttta attggattta aaggaaataa gcttgaaaat tatgaggaca tattatttca
    30721 agaggtagag gaagaagaat atgaagaata tgaagagttg gaagaagaag acgaagagat
    30781 agaggataat gaggaaagtg atagagacga aggtagtgaa gaagaatata ttatagaaga
    30841 atatgatgaa gatgagtatg aagactattg ggaagatgat gaagatattg acgaaggaga
    30901 gttaaaagca agaacggaag tagaagattc tggtgatgag gaaatagaag agttcaataa
    30961 ttataaggaa atagaaaatt ataatagggt taaagtacag gaagaatata tagacataga
    31021 atatgatgag gaagaagagg aagaagaaga agtttttgaa gtaaatagag aagaagatag
    31081 gtttgtttcc tcaaaaccta ctaaatcaaa gaaagttcaa aaagatcaga ataatattga
    31141 aaaaataaaa agagctaatg gagtaagtca aggagagagt ggaaataaac aaaccagtac
    31201 attacttatg ccaagacaaa taaaaaaagg tttgaaatta tttaaagaag taaaaccttt
    31261 tgtaacagat tatatagata agacaagatg gtggaaaata gagataaatc ctacaacatt
    31321 gtgtggatat acaatgcctt atttaggata tttaaacgtg ttgaattaca ccatgtatag
    31381 tgatgcagtt ttacattcat ataaatacag acactattta tttggagtcc agtatgatga
    31441 atataataag agaaaacatt atatttatgc tatcccagga aatagaaatg aacagccaga
    31501 caatggaagc acaggatttt taagatatca agcttgtgat aatagaacaa atagtttagg
    31561 atattggcta tgttttatag attctagaca aagaaatata gttaggtaaa aataataaaa
    31621 atgaaaaaat ttagactcaa ttcttatttt actgaaaaaa ttaatgaaat gtagtacaaa
    31681 actttaaaaa ctattgataa tatttaaata ttacactaca atatatcttg aaatacctat
    31741 tatattatgg aggaaaatta tgataaacga gcaaagaatt ttaaatgagt ttcttgaatt
    31801 agtacagata gatagtttat ctttaaaaga ggggaatgtt gctaaagtat tagttaaaaa
    31861 attagaagaa ataggttgtt cagtagtaat agacaatgct ggtgaaaaag caaatggaga
    31921 aactggtaat ataatagcta cattaaaagg aaataaagaa ggaaagaaaa tattatttag
    31981 ttcacatatg gatacagtaa ctccaggtat aggggtaaaa cctatagtgg atgaagcaaa
    32041 tggaataata aaaagtgatg gtactacaat attaggttct gatgataaag caggtatagc
    32101 agctatatta gaaggcttaa gatatataaa tgaaaataat atagaacata ctgatataca
    32161 agttgtattc tctatatgtg aggaatgtgg attagttgga gctaaaaatt tagactatgg
    32221 aaaaatagat tctgaatatg catttatatt agatagtggt ggttctccag gtgaaataat
    32281 agtaaaagct cctgcacaag atgtaataaa tgttaagata ttaggaaaga ctgctcatgc
    32341 aggtcttgaa cctgaagctg gaataagtgc tataatggta gctgccagag caatagaaaa
    32401 tatgaattta cttagaatag acgaagaaac aactgctaac ataggaatta taaacggtgg
    32461 aacagctaca aatatagtaa caggagaagt taatataatt gcagaagcca gaagtctaaa
    32521 agaaaataaa ttagatgttc aaactaaaca catggtagag acttttgaaa aagctgcaaa
    32581 agattttgga gctcaaattg agatagatgt caatagagca tatacaccaa tagatgttag
    32641 tgaggattct gaaataataa aacttgcaaa gaaggctttt tcaaacttag gtatagaagg
    32701 tcatactgaa tctacaggag gaggtagtga tacaaatata ttaagtaaga atggtattga
    32761 agctataact ttaggaattg gtatgaaaaa tgctcataca ctaagtgaac atatagctat
    32821 aaaagactta tatgattctg ctaaaatggt agtggagata ataaaggaag cataaattta
    32881 aataatagtg tataatatat atatcgccta gcttaagcta ggtgattttt cattttatag
    32941 aagattgata atctctattg aaagtaaaga gtatacattt gcatataaga gagtgtttaa
    33001 tttatgttca aatgtaggtt cttaataata tttagattgt ttcataaggg aggaataaat
    33061 atggaaacaa ggtatgaaga agcaaataaa ataacaatac aatcaatact ttggaatgta
    33121 gtattaacaa taattaaggt tatagctggt gttattggta attctagcgc tatgattgcg
    33181 gatggattac attcagcatc agatattata agttctatag gagtactaat aggcaattat
    33241 gtatcttcta gacctggaga tagagagcat aattatggtc atgagaaagc agagacatta
    33301 gtatcatttg tgttatcaat attgcttatt ttcgtttcta taacaatagg tatagaggca
    33361 attaaatctt tatttaactt agatgcatta agtgttccat caatattacc attagtagta
    33421 tcagttattt caatcctgat aaaagagtat caatatagaa taacaataaa agttgctaaa
    33481 aagataaatt caccagccct aaaagctgat gcttggcatc ataggtcaga tgctttatct
    33541 tcagtagctg cttttatagg tataggtggt tctattttag ggtttaaacc attagaccca
    33601 attgcgtcag ttgtagtggc tatatttgta gctaaagtag gaattagtat acttataagt
    33661 tctgttaatg aattgatgga tgtttcagtt gatgaagaag agataaaaga attaaaattt
    33721 attgtagcag atacagaagg tgttaaaaat cttggtgata taaagactag aaaacatgga
    33781 gctatggcat atgtagattt gactatatgt gtggatgaaa atttgacagt taagcaaggt
    33841 catgatattg ctaccaagct tgaaaaacat ataataaaac atatggaatt tgtaaaaggc
    33901 ataacagttc acgttgaacc atgtactaat tgccaaggaa ataagtgtaa taattaatta
    33961 aaacttaaaa aaaagtgtaa aaagttatat aattgtttta tgattataaa ctctaggggt
    34021 ggttaaatga gcaagaaaag aaaactaaag aaaaaagctg caatattaat aggagtagtt
    34081 gttttcttta taatatactt tatatccttt agaataggaa taaatatgga aaaggataaa
    34141 agtgaagtgc caaagaaggt agaaaaagaa gaagaagata ataggtcttt aaggactcaa
    34201 ataaaagaga agaagaaggt ttatatatca gataaaaata tagaaaacat aaaaattgaa
    34261 gaagatatgt gggatgaaat aaagttattt ttctcagatt ttaagaaagt aagaaatact
    34321 acttcttctt acgaagcagt ttatgaaggt tattctgatg atggagttaa attttctact
    34381 gaccttaact tttttagagt atatactgta aataaagaag aatattataa agtaccagtt
    34441 agttctaagg atgcttttaa gaaactttta gatgagagca tatatacttc gtttgacctt
    34501 ttaagtcatt ataaagattg gaaagaggtt actataactc atggaaaaga aattaaaaaa
    34561 gttcatagat ggaaatttga tgatttagca cataagatga tttcaaagag ggttgtagga
    34621 aaagttcaac ctgagaaaag taaggaaaga agtgactata attttactat aaatataaaa
    34681 ggtgataatt ttgatgtaaa agttgataca atgggaaaag attatgtaaa agttacttct
    34741 aagaaaaatg tatcttacta tgaggttcat acaagtttat ttgattacct taaaaatgag
    34801 atatttgaaa ttggaaagaa ataagacttt aagaaggcta tcaaattaga tatttgatag
    34861 cctaaattat tttatatatt atttttcaac caacaagtct gcaaccttat aaattggacc
    34921 agcaccagca gttattacta aatcattatc cttaacattt tcacgtaagt attttactat
    34981 atcttcaaat tcttttatat agatggcatc tacattgttt tgatataatt tctcaaccaa
    35041 gtccttagaa tgtatatctc ctgggtcttt ttccctagca gcatatatat ctgttatgat
    35101 aactttatca gcagcataga atgcctctga aaattcacct aaaagagatt ttgttctagt
    35161 ataagtatgt ggctggaaga tacaccataa agttgatttt tttaattttt tggcagcagc
    35221 taaagttgct tttaactcag ttggatgatg tgcataatca tctactacta aagcattctt
    35281 ataataccct ttagtttcaa atcttcttcc gacaccttta taaattttta tattttttct
    35341 tatagtctct aagtcaatac cagaaacaag agctgccatt atggcagaag aagcattgta
    35401 tatgttatga agaccatata cagaaagttc aaattctcct aaatctcttc cttcatattc
    35461 tattctaaat attccatgac cactattgtc aaagtgaata tcttttatta cagcatcatt
    35521 gtcagaatct ctgccgtatt ttataatagt agctttaaca tcatacaata tgtcatcaat
    35581 attttcatca tcgccattta ttatgaaata gccttctggg ggtaataatt ttccaaattt
    35641 gttaaaagaa gctttgattt catctattcc agagaaataa tcaagatggt cttcttcaac
    35701 atttaaaact atagatatct ttgggttaaa attaaggaaa ctatctacat attcacatgc
    35761 ttcagttata aagtggttgg aattacctat cttaacatta ccacctatca tacttaagtt
    35821 accaccaact agtatagttg ggtctaagtc tgcatattca aagattgtag ataacataga
    35881 tgttgtagaa gtttttccat gtgttccaga aactgctata gaatttttat attccctcat
    35941 gatttggcct aaaaaagtcg ctctgttcat taccaattta tttttttctt tagcagccac
    36001 aagttcttca ttgtctggat gaactgctgc tgtataaaca accatattta catcatctga
    36061 tatattatct tttttctgac caatataaat agttgcccct tggtccctaa gtttgtctaa
    36121 taggtatgat tcgtttgaat ccgaacctga aacttgataa cctttattga tacatatttc
    36181 agctaatgca ctcatgctga taccgccaat tcctataaag tgtatattca taataaaacc
    36241 acctttcttt ttaattaagt cctacaatat gaactatttc tgtggtataa taatataatg
    36301 ttcaatattt tataattaat atgcatataa tattatatgt attttatttt tgaatataat
    36361 aaaacataag taatattata ccacaataag aattgttatg cattagttag tttaacgtta
    36421 tataaaggta cttgtaggag gaaataataa tgaaatttaa aagaacagag agaattggag
    36481 caatagttaa aatactgtct gataatccaa ataaaatata tactttaagt tattttacaa
    36541 atcaatttaa tgcagcaaaa tcaacaataa gtgaagattt attagttgta aaaaatgtat
    36601 ttgagaagct acatttaggt aaagttataa ctatttcagg agcagcagga ggagtaaaat
    36661 atatacctaa aacttctata gcagaaaatg cagagttctt aatggaatta tgcgagagaa
    36721 tatgtgacaa gtctagaata ctatctggtg gttttttata cttgattgac ctaatttacg
    36781 accctacaat agctgctaag ataggaaaga tattcgcatc aaatattgag tatgtagatg
    36841 cagattatgt tgtgacaatg gaaacaaaag gtatacctat ggcacttatg actgcaaaag
    36901 ctatgaattt acctctagtt ataataagaa aagatataaa ggtatcagaa ggtcctacac
    36961 ttagcatgac atatgtgagt ggaaacagtt ctaaagttga aagtatgagt ttgccaagaa
    37021 aagctcttaa accagatagt aaagttataa ttatagatga tttcatgagg ggtggaggta
    37081 ctataaaagg aatggtagac cttatgaatg aatttggtgc agaggttata ggtactggag
    37141 tattcatatc tacaacgaat ccttcagaaa aaatggtaaa agattatatt tcgttgattc
    37201 aattggatgt taatggagat aaaatagtag tagaaccaaa cttaaagact tttaaagatg
    37261 aatatagaaa tgaagattta gacgaagaag aagattcaga atttgaattt gagatagatg
    37321 aagattaata aaaaaaatat ttagaaaaat atgtaaaaat aaaaagagga tatccctagt
    37381 tgttcataga attatttaaa aaacagttta tattaattta ttccagcgat aataatttta
    37441 atataaatta atttctgtca aaatcaaagg gggatatttt ggtatgaaga taactgacgt
    37501 aagagtaaga aaattaacag aagaaggtaa gatgaaatgt atagtttcaa taactttcga
    37561 taatttgttt gtagtgcatg atataaaagt tatagaaggc cataatgggc tatttatagc
    37621 aatgcctagc agaaaagtag gcgaaggaaa tttcagagat atagctcatc caataaatgc
    37681 agaaatgaga caagttttag aagatgcagt tttacaagcg tatcatgaag cattagttca
    37741 atgggaagta gctgctgaat aataataaat atataaaatg gagagagccc gaacatttag
    37801 gctcttttta aatgtagaat ttctattaaa atatgtactt tttagtaaaa aagtgctata
    37861 ttattagtga taatgtatat gttgtataca aattgttaga taaatgtatt ataatgtata
    37921 ttgattaaga gaataatacg aataaaatta aattagattg aatggagtgt atcaatgaac
    37981 tttaaagcta taatccttgc tgctggcaaa ggaacaagaa tgaagtctaa atacccaaaa
    38041 gttatacata aagtatgtgg aaaagaaatg gttaaccata ttatagatgt ttcaaaaaaa
    38101 tcaggggtaa aagatactgt cgtgatttta ggacatgaag ctgatgtagt taaagaaaag
    38161 cttgcagaag aaattataat agccatgcaa acagaacaac ttgggacagg acatgctgta
    38221 aagatggcta aagaatatat taatgatgaa gatacaatag ttgtactttg tggagatact
    38281 ccacttataa aagaagagac actaaagaga ctatttgaat atcatataga aaataaatat
    38341 catgctacag ttcttacaac aagagttgga aatccaacag gttatggtag aataataaga
    38401 gataagaagg gtgaccttct taaaatagtg gaacaaaaag atgcaaattc agaagaaaag
    38461 atgataagtg agataaactc tggaatttat tgttttaatg gaaagagtct tagagaagca
    38521 ctagatttgc taaataataa caattcacaa ggtgaatatt atttaactga tactgctaaa
    38581 ataatgagag ataaaggatt aaaagttggt gcatttgcag gttcaactat tgaagaactt
    38641 atgggcgtta actcaagagt agaattatca aaggcagaag aaattatgag acgaagaata
    38701 aatgaatctc atatggtaaa tggagtaact ataatagaca ctaactctac ctatatagaa
    38761 tctgatgtta tgataggcaa tgatactata atatatccag gtgttatgtt acaaggaaaa
    38821 acaaggatag gttcagattg tataattgga atgaattcat ctataacaaa ttcagaaata
    38881 ggtgatggta cagaaataaa aaattcaact ataattgata gtaaggttgg agaaaattct
    38941 actgttgggc catatgcata cttaagacca aagagtgact taggaaataa tgtaaaaata
    39001 ggcgattttg tagaagtaaa aaatgctatt atagaagatg gctcaaaagc atctcacctt
    39061 tcttatatag gagatgcaca tgtcggtaaa aatgtaaata ttggatgtgg agtagtattt
    39121 gtaaactatg atggtaaaaa taaatttaaa tcaatagtaa aagacaatgc gtttataggt
    39181 tcaaattcta atttagttgc accagtagtt gtagaagaaa aaggatatat agcaacaggt
    39241 tcaacaataa cacatgatgt cccagatggt gctttggcaa tagcaagaga aagacaagtt
    39301 atcaaagaag gatgggtaga aaaaaagaat caaaaagacg accaaagtaa gtaattatgt
    39361 agtttattat tttggctaaa aaatttagct cgaaattatt ataaattata ttcattagta
    39421 ttttaaaatt ttcaggaggg ttacataaat gaatactagc ggaagcgaga ttaaaattat
    39481 tgcaggtaat tcatcgaaag agttagcaca aaaaatagct gactatatag gtgtatcagt
    39541 actagattgt gaagtaggta catttagtga tggtgaaata tgtgtaaaca tgaatgagac
    39601 agtaagaggt tgtgatgtgt ttgtagtgca atctactaac agcccagtaa atgataactt
    39661 aatggagtta ttaatcttaa ttgatgcgtt aaagagagca tcagcaggta gaataacagc
    39721 agttattcct tactatggat atgcaagaca agacagaaag gctaaggcaa gagatccaat
    39781 cactgctaaa ttagttgcaa atttaataac tgctgctgga gcagatagag tattaacaat
    39841 ggatttacat gcagctcaaa tacaaggata ttttgatata ccattagacc acttactagg
    39901 tggaacaata ttagcaaatt attttaatga aaagaaaata gaagatttag tagtagtatc
    39961 tcctgactta ggaagtgtta ctagatcaag aaaatttgct aatactttaa atggagaagt
    40021 tccaatagct ataatagata aaagaagacc gaaagctaat gtatgcgaag ttatgaactt
    40081 aataggtgat gttaaaggta aaaatgttat actattagac gatatgatag atacagcagg
    40141 aacaatagtt aatgctgcaa atgcacttaa agagtttgga gcaaaagatg tttatgcttg
    40201 ttgtactcat ggtgtgttat caggtccagc tattgaaaga attgctaatt cagaaataag
    40261 tgagttaata gttcttgata ctatacaact tcctgaagag aaaagaatag ataagataaa
    40321 gataaaaaca gtagctcctt tatttggaga tgctataaga atgatattct ctaatgaatc
    40381 agtaagtaaa ttattctaat ttattgtatt tatgagagtt gtttaaaagg atataaacct
    40441 tttgagcaac tcttttttga ttttataatt atggaatgtt atttttttct tgttagaaag
    40501 gtatatatta ttgtaaaaag agattatagt ttaaaattat gtatatttaa gtaaaataaa
    40561 cttaataaag ggcatattat tcaaaatatt atgaatataa aaaatattac aaaaaagtat
    40621 tgaaaaaatt cagattatgt agtattatgt aaatataaat aaagaaagaa agaggtgttt
    40681 ccaatgggaa ggttagaaga tgaaccccta ataattaaat gattcagtca caatctaaag
    40741 tgaatataaa aatctattga aagtttattt ataagaattg aaaatagtat ctattctatc
    40801 tttagtgttt gtgagagggt tctggattat ataagattac tgtaaagaag taattttgca
    40861 caataagcat atctgtaaat gaagagtttc gtttatataa tgattgtggc tgaatcataa
    40921 ctataataga tagaatgttt ataatagatg attttataaa atactatatt gtaatagctt
    40981 ttagctttca aataaatatt agcaaaggat ggaaatgaca atgataaaac taaatgataa
    41041 tttataaata tctccttacg atatataata attgtaggga gatgtttctt ttttttgtag
    41101 agtataattt ttctagaaat tctgtttcaa tatgtaaaaa gttggtatat ataaatagta
    41161 taaacagtgt tgctaaaata tggtagatat tatataatat aaatggatat gtatatacat
    41221 tatgagttat ataatttgaa tttaataaaa tagaaacgtt gatttatgtt ctgtaatgtg
    41281 ggcaccttgg agcatattga gttagtggtg caaccggcta tgaaattgta tttatttata
    41341 aatactatta ttttcatagc tttttttatt taaaaaatgg ttatatgtta gaaataaatt
    41401 tggttagtac gtttaaataa ttttacttag atttttaacc agatatatta taagtaagta
    41461 ttaattaaaa aatttaaggg ggaataaaaa tgaagttaaa aaagaataaa aaaggtttca
    41521 ctttagtgga attattggta gtaattgcaa ttataggtat attagcagta gtggcagttc
    41581 cagctttatt tagtaatata aacaaggcta aggtagcaag tgttgagtct gattatagct
    41641 cagttaagag tgcagcatta tcttattatt cagatactaa taagatacca gttacaacaa
    41701 ctgataaagc tggtttaact attttagaga cttatatgga gtctttgcct gataaagctg
    41761 atataggtgg aaaatataaa ttgattaaag ttggtaataa attggtatta cagataggta
    41821 cagatactga aggagttaca ttaacagaag cacaatcagc aaaattattg agtgatatag
    41881 gtaaagataa aatatataca ggtgttacag gagataattt tggagacgaa ttaacagata
    41941 ctacaaaaat agataataaa gctctatata tagtacttat agataatact gtgatggact
    42001 caacaaaata attaaaacta tatttttata aagaatattg taaatttaat gaaaaatata
    42061 atataaaaga caggaagaaa atcttcctgt ttttttatac tattttcttt aatgttcaat
    42121 tatgaatgat gatataattg agatagtaaa ggagtgaaac ctgtggctaa aaaagttaga
    42181 ataggagaca aactggtcga aaaaggatat ataacagaag aacagcttaa atgggcacta
    42241 tcagaacaaa aaaacagtgg aaaaagacta ggagaatttc tagtacaaga agggcttata
    42301 gatagtaact tactaataag tgtattaaaa gaactcttgg atatagaaag tatattttta
    42361 gaagggacag aaatagacac tttagctaca aaaatggttc cagaaaatat atgcaaaaga
    42421 tatactgtat ttccatttaa aattgatgga aataaaatat gtctagccat gtcagaccca
    42481 caagatagag aagcagttca agatgtaagg cgtatgtctg gtaaagacgt agagatattt
    42541 atatcaagta cagaagatat aaacaaagca ataggtcatg cttatgcaca ttcagaaata
    42601 aataaggcaa tgactgagta caacaaaaat agaactggtg gagtaagaga aacagtaata
    42661 cttgaagaag atgtaaatgc tgctccaata gtacgattag taaataacat actagaaaat
    42721 gctgttagaa tggaagcaag tgatatacat atagaacaaa gtgaaaatta catgcgtgtt
    42781 aggtttagga tagatggaat gttaagagaa tatatgagga tgaactcagc accatataag
    42841 gcagtaatca gccgtatcaa gataatgtca gatataaata tatctgaaaa gagaatacca
    42901 caggatggta gaatctattt aaaagtggat aataaaccca tagattttag ggtttctaca
    42961 atgccaacta acagggatga aaagatagct atgagggttc ttgataagag taattttatg
    43021 gttagtaaag aagtactagg aatagacgaa catggctcta agatatatga tgaattgata
    43081 aacactcctt atggtcttat attggttgtt ggccctactg gaagtgggaa aaccactaca
    43141 ttgtattcaa tgttaaatca actaaataca gaaaatagaa atctattaac aatagaagac
    43201 cctatagagt atgaattacc tggtgtaaat caatctcaaa taaatgaaaa ggcagggctt
    43261 actttcgcca gcggacttag agcttttatg cgtcaagacc cagatataat aatggttgga
    43321 gagataagag atactgagac agcagaaata gcaattagag cttctcttac aggacattta
    43381 gttttaagta cattacatgc aaatacagca gttggagcaa tttcacgtct tttagatatg
    43441 gatgtggaaa gttttttaat aacttcatct gttttaggtg taatatctca aagacttaca
    43501 cgaaaaatat gtgagcattg taaggtttca tatgaagcag atattggaga aaaaaaggct
    43561 cttggtattg atgtaaatga atcagtaact atttatagag gaaaaggttg tgaaaggtgt
    43621 aataatactg gatataaagg acgtctaggt atatttgaaa tgcttgaaat tactccagaa
    43681 ataaaagagt taatagattc atctgcaaat caaagagaaa ttttaaagat ggctagaaaa
    43741 caaggtatgg ttagcttaaa agaggatata gttaaaaaag ttttaaatgg aaagacaaca
    43801 gtagaagaaa tgattagaat aattttgatg actgattaaa ggatgatatt atgaaaagtt
    43861 ttaaatacac agtaataaaa gaggacggaa aaaagcaaac tgatgtgatt gaagccaacg
    43921 attttaatga agctagaaat cttcttagaa agagaaatct tagagttatt gaaatcaaag
    43981 aaagcaaaaa taaaaatata agattatcta gtagaaagag aaaaaaagac ttaggagctg
    44041 accagataag ccacttttgt agacaatttg ctattatagt atcttctggt ataaatagta
    44101 tttctggatt agaaacatta gctagaaggt caactaacat tacattaaga gaagaaatta
    44161 atcgtatagt agcagaaata aaagtaggtt ctactattgc agattcaatg ctttcatcaa
    44221 aatcaaagtt tccaaagtta cttggtgcta tggtagctac aggtgaagct acaggtaaat
    44281 tagatgaagt attaaaaagt atggcttcat tttatgcaag tgaacacagg gttaaacaaa
    44341 agcttagaaa tgctgcaaca tatccattaa tagttttgat ttcttcattt ataatgattt
    44401 ttatatttac aacatttatg gtgcctaaga tgattaattc aattacaaca gtaggagcaa
    44461 ctcttccact tataacaaga attgttatgg ggtttggtat gtttatgaag aagttttgga
    44521 taatagtact tttagttatt atattattta tctatcagtt taaaaaatat ctaaaaacgt
    44581 cagttggaag ggctcataaa gatagagtta taaataagat acctgtttta ggaaaaggaa
    44641 taaattgtat ggtagctaca cgattttcaa gagcattgta tctatttgta tcaacaggat
    44701 acccattagt tcaagggctt gattatatta tagatagtgt aaataacact atggcagaga
    44761 agattttagc atcagctaaa gatggaataa caagaggaga aggcttagca gaaaatttag
    44821 agagatatac gtattttgac tctgtgttgg tacagatgat agctataggg gagcagacag
    44881 gggaattaga gaatataagt agacagatgg cagaatttta tgagtatgaa tccgagattt
    44941 atttaaatag gatggcctca atgatagagc ctgttttaat aattgcagtt ggtattatag
    45001 ttgcgatact ggttgtatca atattcatgc caatgcttag tatatatgat gccatgtagg
    45061 agggaaagga caaatgaaca aggtttatgt atcatattat ggcgattcta tttccatagt
    45121 tgagggcaga tataaacacg ataaggataa gttttatgta aaaaatttta atgtgttttc
    45181 aatagaggat attgcacaag atttttctaa ggaaaagtat gctctattga gatacgcatt
    45241 agaaaatgtt aagttaaaat caagacatgt agttttttgt ttaaatacaa gagatgtaat
    45301 tttaaaaccc caaaaacttc ctaatatgga caaaaaggat ttagacggat ttatgaagct
    45361 agagatggac gaaatgatgg ctttggagtc agatgaatat gtattttcgt atgaagtatc
    45421 tagtgaaaca cctgataatt cagatggcga tgaatcaagc ttaaatctta ttattggagc
    45481 tatacaaaag gaagaggtaa atgcaattgt agagataatg catgaatttg atttagtatt
    45541 agatagaata gatacattat caacagctta cttaagaatg cttaaaaatt tagactacaa
    45601 tgatataatg gtaaccaata taagtgacaa tagcacaatt ataaatatat ataagaaaga
    45661 tactttattt attagtgata atatacctat aaagataact gctaaagata tggacatgca
    45721 attattaaat gtagtagaag aatcaaaagg tcttatgaat tactattcgt caagaaactt
    45781 tggtaaggtt acggatacta tattaatatt aggaaaaaga tattccaata aattaattta
    45841 tgaaactttt agctctgcat tttcaagcta tgtttctcaa ggccttactg agataatgga
    45901 tatatctgat atgataactg gagatataga tgaagatgat ataaattcaa ttgtagagct
    45961 tttaggaagt atgatagaac ctaaagggaa aagagatttt gaaaatataa actttttacc
    46021 aatggatata cttagaaaac agataaaagc taaacaaatg aaaaacatac tgaaaattgc
    46081 tccaatagtt attttgattc ttgctatacc ttatttatgt ttaatttttt caaataattt
    46141 agcaaataag gaacttgaaa atgttaatgg tgaaataaga aatattgaat ctgcatatta
    46201 tcagattgga aatatagaaa aaatgataca gagtaaaagc gaagaaataa aggtatatga
    46261 tatgttaatt gggaaaaagg taaagtggga aaatttattg gatgatatag ataaaaatac
    46321 tccaagtaaa gttgaactaa caagtttatc aacaacttat caaaatcttg gaactaataa
    46381 taaagctgat aattcaaaaa gtaataagac tacaacagat aaatcaagta acaatgaaac
    46441 aaaatcaagt acgaaaagtg atagtaatgg agataaaaca gataatgatt taaatgaaga
    46501 taagactaca aatgtgtcag aagcattaac tggaaatagt gagactaata gtaataacac
    46561 ggataaagat gataaaactc cattgtatga taagatacca aatgttataa atatagaagg
    46621 aaatgctaaa gattcctctt atgtaggtca atttttatat aaattaaagg gattatacta
    46681 tttcaaggat gttaaattac atagcataaa aagtaatgca gaagattcta actatacatt
    46741 tagtatgaca ttatacctga aagaaggtgt attaactaat gaataaagat atatttaaaa
    46801 aagatataaa agatatattt aaaatggaag ttagtctaaa agggattctt ataaaggtta
    46861 taattttatt agctatttta tcatatggtt attttggact ttaccctcaa tatcagaaat
    46921 atattgctac aaaaaattct gtaaaacagg ctactgaaaa attaaatata tataaagaga
    46981 aaactgcact tatgccaaaa tatgaaaaac aacttgagga agttgaaagt gaattaaaag
    47041 aaaaaagcaa aaatctttta tatgatatgc aagatggtct ttttttaata ggtttagata
    47101 aaaaaatgaa ggcttataat attgacttaa ccagttatga cgttggaaat gttaaagaat
    47161 acgaacattt ttatggtata tcaacaacaa taagtgttag aggtgattat agaaatgtca
    47221 aaaaattaat ggcttattta gaagaacaaa aaaatgttac tcaaatacta gattatacaa
    47281 tgacttcata tatagaacca gaaaaagaag aggaagaaac aacaaacaat aaggatttaa
    47341 ttaaaaatgt gaacataaat aaagatacaa aaatatactg ggtggaaggc gaaaataagt
    47401 ggcatttata taaggattgt ccattgataa aagagggaga gattataaaa aatgacaaac
    47461 ttttagatag gtctactaat tatctatgta atacttgtgt acttagagct gcaacagaaa
    47521 aagaagcaaa aaaagaagtc gtagctccaa aggcagaagg tgtagtagat gctacattta
    47581 aatttataat gtacactaaa gagaatacta caatgatact tgatacagat aatccatctt
    47641 catggagacc aggaaaatat aatccattta agtctagtgc gaaataaagc taagtaagct
    47701 ttaaagatag gtgatgagtt tgtacaaaaa taatgaaaaa ggtcttactt tattagaagt
    47761 aataatagct gtttttatac tgacaatagt tttaagtatt tcttataaag tgttcaatgg
    47821 tataacatca gcagtaaaaa agcaacagat tattacagat gctcaagtaa atattaatct
    47881 aattaataag tatctaaata gagatttgga aaactgtaaa gagctaacta aaactggttc
    47941 aggtaataat tatgaataca atatagagat gccagataat gttgtaaaat atgaagtttc
    48001 tatagaaact aaaaaaaata cagaggtata ttctgtaacg agaatccaaa acaatacaat
    48061 tgatacagaa aatgaagtta gagaagagat aatctataat caaccactag ttcaaaataa
    48121 taaagaaatg aaggaaacac catttaaaat agaaaagcaa actggtaaat ctatttatac
    48181 tgtaagtata tactataatg aatcggtgca agaaagtcat aaaaatagca atttaaataa
    48241 caaaacttat acatttgatg ttatgtcaag aatagggtaa taagaattta ttaaaaaata
    48301 agtaggttga taaaatatga gtaaaaagaa tagtgaaaga ggattttcct taattgaagt
    48361 gttagtagct atggctatta tgggaattgt actatttgca ttttttaata ttatcaatac
    48421 taataacaaa gcaaacacta aaaatgacac agatataact tccttaaact atgtccaaag
    48481 tgaaatagaa aatctaagag aaaagataaa aagtggagaa tttgattttg atagtttaga
    48541 taaactagaa gatggaactg tcgtatatga aaaattaata gataaatcaa aaaaagttgt
    48601 atatgataaa gttcttagtg aaggtgatgt gagcttatat gatactccat atgaaaagat
    48661 tacaacaata aaagatgaag atggtaattt aatcgacaaa ggaaatatta caaataagat
    48721 aaagactatt gtagaagata aaagtggtca gatatataaa atagctgtaa ctgggaaaag
    48781 catgaatgac tattcaagta aaaaagaggt taaaattgta actgaaatat ttaaagataa
    48841 ataattttac ttgggggtga gattttgagg aagtggaata aatttaaaag tgaaagaggg
    48901 gcagctttag ttttagtttt ggttgtagta gcattgcttt ctattgttgg attgatattt
    48961 tcaaatcaga tagctaatag aataaaatct acaaagacta ctaatgaagg tatacaagca
    49021 aagtatttag cagagacatg tgtggaaaat agtattgata aagcatatga aaaattgtat
    49081 gatgagcttg agaaaatgga taatgagttt aaaagtgaaa atcaagaaaa atccatatct
    49141 aggtcaaaat taagaaatat atcagatgaa gattttaata atcaagatga ggaaaatatt
    49201 gaagcagaaa gacttggata tatgaataat ataaatttct atttaaataa agcttcttca
    49261 gacttggaaa aagctagtat ggaattgaaa aagctatatg atttagatat gttggactat
    49321 agagacattg aatatgtaga tgcaaatatt atttcacata gaaattctat cttagaaatt
    49381 tgcaagaatt atacttctgg agatattagt aaaataaatg aatatattct gaaagaagat
    49441 atagactcaa ctacattgat tgaggcaaag ttagtgaata atgatattct tttaaaaaag
    49501 tttttagaag aaaataaaat tgaaaatgaa catttaaatt cagccttttc acatacttat
    49561 aaggcattag ataacatttc tttggcgatg caaaatatga ttgagtatag acatacgttt
    49621 catatagatg agccaaaagt agaagtatct aatgggattc cagactctca acaatattat
    49681 gaactaatac aaaatcctat aataaatagt atggagtata gctggaatag taaatgggat
    49741 acattagaaa acctattgga aattttacca aatcaaactc aaggatttaa ttctttaaga
    49801 gttcatttga gaaataatgt aagaaaattt gagaagttaa gcgataatat atctagtggt
    49861 aaaaaaaata cagcaaaaaa tttcttaaag tataaagagt tattgtatga aatatcagac
    49921 caatgtaatc aactaaaaag tatgtcttat gagaaaatac ctgttaaata tgataacatg
    49981 gctttgataa caacttttga ctatatacaa aatgagcttt tagcagagat aaaatgcaga
    50041 ttaaaagaat taaaaccaca agaaattgat aaaacagaag gtattactat aaaaatacct
    50101 ttttataagg ctgactacga catgacaaaa gaaggatggc ctaaattaaa agaaaatggt
    50161 tcaggggcgg agttaagctt aatggtaact ggtgataaaa atggaataaa agaagtagaa
    50221 gttacagatg gtaaaaaaaa tattattggt ttaggtgttg aagaaaattc aaattcaaag
    50281 tataaggttg acgccattgt aaactttaat ctaaatatag atacaaatgt agttggaaat
    50341 tatgatataa aagataaaat tttgattaat catgatataa gttcatacaa aaaagtaaac
    50401 taaatataag aagcaactta gtataaataa ataataaaat aattttatta tttactacta
    50461 aaaaaataag taaactaata gaagatatgg agagtaaata ttatgtatat ttatgattta
    50521 ttagagcaag gtataagact aaacgcatca gatatacaca taacagttgg aacaaatcct
    50581 gtagctagag taaaaggtgg atttgtaaaa ctaagtgaac aaatattgac atcagaagta
    50641 acaatgcaaa tggcaaaaga catagcaggg gaatctatgt ttaaagtaat agaagaacat
    50701 ggagaagctg atttttcagc ttctctaaaa acaggagaaa gatttagggt aaatgcatat
    50761 aggcaaaaag gaaattacgc aatagcaata aggactataa cagcagaaat tccaacattt
    50821 gaaaaattag gccttccaga gagtataaaa tcttttactg aaaagcataa aggattagtt
    50881 ttagttacag gtccaacagg aagtggtaaa agtacaacac ttgcaagtat gataaatata
    50941 ataaatgaaa aacaacaaaa acatataatt acacttgaag acccgataga atatgtacat
    51001 catcataaac aaagtttagt aaatcaaaga gaagtaggaa ctgatacaga aagttttcat
    51061 tcagccctta gagctatttt gcgtcaggac ccagatgtaa tactaatagg agagatgaga
    51121 gaccctgaaa ctgtttcaat agcattaaca gcagcagaaa caggtcattt agtattttca
    51181 acactacata cagtaggggc agctaagaca attgatagaa tagtagacat gtttcaacca
    51241 tcccaacagc aacaaataaa aacacagtta tctacagttt gtgaaggtgt ggtatcacag
    51301 cagttacttc ctacagcaga tgggaaaggc agaatagcag caattgaatt gatgtttgct
    51361 actccagcaa taaaaaacct gataagagaa ggcaaaacat atcaaattcc aaacatgata
    51421 caaactggag ttaaagtagg tatgaaaact atggaccaag atttaatgga actttataaa
    51481 aatggaaaga ttacaaaaga tatggcactt agtaggtgta ctgaccaaga gtttatgaca
    51541 agaatgatag gtggagtaaa ctacaatgga tattataatc gttaatctat atgtatttat
    51601 agtgggtata gtgtttggaa gtttctttaa cgtctgtatt tttaggattc cagaaggtat
    51661 atctatttct agaccacctt ctcattgtat gagttgtaat actagactaa agccaataga
    51721 cttaatacca gtgctaagct atttaatgag tggcagaaag tgtagatatt gttatgagaa
    51781 aatttctagt agatatgcta tagtggaatt aattacagga cttttatttt taacagtatt
    51841 tatgttatat ggaatatcta catcaactat ttattatttg gttcttgtgt ctttactgat
    51901 tataattaca tttatagata ttgaccactt tataatacca gataaaatat tggtttttgg
    51961 agctatattt tctatgattt ttaacctttt atttaaagaa attccagtta aggattcaat
    52021 tctaggtgtt tttatttgtg gaggaagtgt ctggattata gttcttttga ttgaatttgt
    52081 aataaaaaaa gagtgtatgg gtggaggaga tatcaaacta tttgcaatgc taggttttta
    52141 catgggtgtt aaaaatggtc ttttgacagc cttactaagc gtttatgtgg gagcagtcta
    52201 tggaatctgt gtaataatta taagtagaat aaaaggaaag gaatataatt ctgttatacc
    52261 atatggacct tttatatcta ttggagcatt aataacaatc ttatctggaa agcaattact
    52321 tgaactttat ttttctatgg ttatatagat gatagtcaag ttaaataaag ttttaattta
    52381 aaaaaatgaa ttaaagtatt taataaggac aatattatta ttaaccctat tactaaagtg
    52441 ctacaaacta tggagtatat aataataatt tccttatttt ttgcacattt gataaataaa
    52501 tctctatgtg ttttttgtga tagagaaaag aaaaatttat ataatacgtt ttttatttac
    52561 ataataactg tagttttatc tgtattaata tatctaaaat ttgggattag tgtagaatgt
    52621 gtacaatata tggttttaat tccattttta ataattatat ccattgtaga ttactacaca
    52681 atatatgtat atgatataac aatagttagt ggtataatag ttcaaggtat aatttttttg
    52741 gcctttttca atatagttca atttgattat ataaatcata tatttggact attgataggc
    52801 tttattatac cttatattat tgctaaacta acaaaaggat taggctatgg agatgtaggt
    52861 ctctatagct tatgttgctt tgcattggga aataattatg ggttttatgt gatagtttta
    52921 tcatttattt tagcgttttt gttttttatt attttataca taactaaaat atttaaagat
    52981 gtagatataa gaaagcaaat tccttttaca ccttttattt ccttgtcaac aattttaata
    53041 atatttacag agtataatat atttaatttt tattataatt tgttatacaa atttatataa
    53101 caggaggtaa gaaatgtacg tagtagtagg gttaggtaat ccaggaaaga agtatgagaa
    53161 gactagacat aacgttggat ttgatgttat agatattctt gctaaagaat acaatattag
    53221 tgttactaaa ataaagcata aagctttgat tggtgaaggg agagtaggta ctgaaaaagt
    53281 attattagtt aaaccacaaa cttatatgaa tttaagtgga gaaacattaa tagatatata
    53341 taaatattat aaggttgatt taagtaatat agtagtagta tatgatgata tagatttaga
    53401 agttggaaag atacgtataa gaaaaaaagg aagtggagga actcataatg gcatgaaatc
    53461 tataactaag tgcctaggtt caaatgactt tccaagagtg agagtaggtg tatctaaacc
    53521 agaagctggt caagacttag cagactttgt actttctaga tttagaaaag aagaatctga
    53581 taacataaat gaagcattag aaaaagcggc agatgctatt gactcaataa taagagaaaa
    53641 tatagatatg tctatgaaca aatataatgg atagtgagag ggagatttaa atgaatgatg
    53701 tttttttata tcctttgcaa aattcaaaag agtataaaga tataataaac tgtataaaaa
    53761 atacaaaagg ctcattattg gttaatggac ttttaccagt acaaaaacct catatatcgt
    53821 attctatatt taatgattta tcacgacaaa tgatttttat aacgagtagt gatttagaag
    53881 caaagaaagt atacgaagat ttaagttttt atatggaaga taaagttgaa tatttaggtt
    53941 ttcaagatat ttatttttat cacttggatg caaaggacag aaacgaagaa gcgaagaaat
    54001 taaaagtatt gttaaaacta gcaaataaag aaaaaatcat tttagtaact tctattgagg
    54061 cagttttaag aaaatacata ccaaaacaag ttttgctcga tagtgtatct cattataagg
    54121 tgggagatag cttagactta gaaaagttaa ctgagaaatt agtttcattg ggatatgaaa
    54181 gagtttcaaa aatagagggg tttggtcaat ttagtataag aggtggaatt attgatgtat
    54241 tttcacttga atatacaaat ccaattcgta tggaattatt tgatgatgag atagattcca
    54301 tacgaacttt tgacgtatat tcacaaaaat caattgataa attgcaacaa ttctctataa
    54361 ctccatctag ggaatttatt tatcccgaaa aaactacaga tgcattagtt aaattaaaaa
    54421 aagaaacaac taaaaatact gatgaaggta catttcaaaa tatagattat atatcaagta
    54481 agacttattt tgaaggtgta gagaactata tagattatat ttatccagaa gaaaacaaaa
    54541 gtatatttac ttatttagca gatgatgcaa tagtatttat taatgatata acgagactta
    54601 aagaaagatg cgaaaattat ataaatgagt ttagagaaaa ttataaatta aatcttgaaa
    54661 gaggtctggc tattaaaaac caaggtaaat tgttatatca ctatacagat ttagagtact
    54721 tagtaaaaga caagtctgtg gtattaaact cgctattgcc aaagtctata aataactttt
    54781 ctataaaatc tataataaat tttgaaagta gagaagttcc tacatttaat ggaaaggtag
    54841 atttgcttgt agaagaatta aatcggctga aatataatgg atacaaaata attttagcaa
    54901 caaatacctt agaaagagca aataaattag gaaaagattt attggacaaa ggcttagaaa
    54961 cgaccatttc taaagataga gatattgaaa ttaaatcttc acaagttata attgtacctg
    55021 cacacataaa cagtggtttt cagtataaat ctataaagtt tgttgttata acagataacg
    55081 aaatgatagg tgtttataaa agagcgtcta agactagcaa taaaaaagtt aaaaagggaa
    55141 agaaaataga atccttttta gatttaagtg ttggagacta tgtagtccat gaaaatagtg
    55201 gtgtaggtag atacacagga attgaacaaa ttacagtcaa tgctattaaa aaagattata
    55261 tgaaaatagt atatcaaggt ggagataatc tatatgtgcc aatagaccag atggacaaag
    55321 tacaaaaata tattggtgca gaagtagaaa aagttaagtt aaataagctt ggaacaaatg
    55381 agtggacgaa agcaaaagca aaagttaaaa aagaaattga agatatgaca aaagacttga
    55441 ttgagcttta tgcaaaacgt gaaaaaatac aagggtataa gttttcaaaa gatacacctt
    55501 ggcaggcaga gtttgaatcg ttgtttccat atcaagaaac agaagaccaa ttaaaggcta
    55561 tagaagaaac taaaaaagat atggaatcaa gtaaggtaat ggataggcta gtatgtggag
    55621 atgttggtta tggaaaaaca gaggtagcta ttagaagtat ttttaaagct tgtatggacc
    55681 aaaaacaagt tgctgtattg gttccaacaa caatattggc ccaacaacat tataatacat
    55741 tcaaagagag gtttgaaaat tatcctttga gagtagaagt tttaagtagg tttaaaacac
    55801 caaaacaaca aaaacagatt attgaagatg ctaaaaaagg attagtagat attttaattg
    55861 gaacacatag aataatatcc aaagatataa atttaccaaa cttaggtctt gtagtcattg
    55921 atgaagaaca aagatttgga gtaaaacata aagaagcttt aaagaaaatt aaatctacag
    55981 tagatgtact tacactttca gcaactccta taccaagaac attacatatg tctttaagtg
    56041 gaataagaga catgagtgtt atagaggaac ctccacaaga aagacatcct gtaataacat
    56101 atgttacaga gagtaaggaa agcgtaattc aagatgagat agagagagaa ttaagtagag
    56161 gtggacaggt attttttgta tacaatagag ttgaacacat tgaagaaatg gcaagtatga
    56221 ttcaaaaact cgtaccagat gctagagtgg ctgttgcaca tggaagaatg acttctaaat
    56281 cattagaaaa tataatccta ggatttttaa ataaagatta tgatgtacta gtctgtacta
    56341 caataattga aactggtatg gacatatcaa atgcaaatac aatgattata tatgatgcag
    56401 ataaaatggg attggctcaa ctttaccaat tgagaggtag ggttggaaga agttcaagac
    56461 aaggatatgc atatcttttg tatgaaaaag ataaaacttt aagtgagata gcggagaaga
    56521 gattaaaagc tatcagagaa tttacagagt ttggttctgg ttttaaaata gcaatgagag
    56581 accttgaaat aagaggagca ggaaatattt taggttctca acaacatggt catatggcag
    56641 ttataggtta tgatttgtac gttaaaatgc ttaatgatgc aattaaaaaa gtcaaaggtg
    56701 aacctattgt ggaggaaatt gatgttgaaa tagatttaag tgtaaatgca tacattccag
    56761 ataattatat caaagatgag ttgattaaaa ttgaaatgta taagaaaata gcttctattg
    56821 aaaacaaaga agacatgttg gatatacaag aagaacttga ggatagattc tctgatatac
    56881 ctaaaccagt ccagacactt cttacaatag cttatataaa atcgttatgt aagatattaa
    56941 aaatagaaaa gataagacaa ttaaaagatg aaatattatt agttccaata acaaagtata
    57001 gaactaaaca gaaaataggt tataatatag ttacagaatt agaagaatta ttagaaaaga
    57061 tgtgcaaggt aaaataactt gtaaatttaa gaagggatgt tttaaatttg aaaaaggtaa
    57121 taacattggt aatagctatg attttggtag tatctgtgac tgcttgcagt tctagtaaag
    57181 gagaaactgt ggcaacagtt gaaggtacaa agatatcatc agatgaattt aaaaaaacta
    57241 tagctcttta taaagattct atggaacaaa cttatggaaa agatatatgg gataaggaag
    57301 ttgaaaaggg tgtaaaatac aaggacaagt ttaaagattt aattcttgac caacttataa
    57361 ctacagaagt aatatattct caagctaaaa aagataactt acttcctaaa aaagaggatg
    57421 ttgaaaaaag ctttaaagaa ttaaaagacg ctatgggtaa agatgaaaaa tataaagaac
    57481 aacttaagaa attaggtata gatgatgagt ttttaagaga ccaacaagag aaagatttag
    57541 caatgcaaaa ctatcaaagt aactttgcta aaaagactaa aatatcagat gaagaaatga
    57601 aaaaatacta tgatacacat aaagatgaat ttaaaaaaga tgaggtagaa gcttctcata
    57661 ttttattgaa aacagtagat gataataaca agccactttc agataaggaa aaagctgaag
    57721 ctaagaaaaa ggctgaagag gctcttaaag aagttaaatc aggtgaggat tttgcaaaag
    57781 tagcaaaaaa atattcacaa gatacttcag caagtgatgg tggtaaatta ggattctttt
    57841 caaggggtca aatggttgct gaatttgaag atgctgcttt ttctatgaaa aagggtgaag
    57901 tatctgattt agttgaaact caatatggat accacataat taaagtgaca gacagaataa
    57961 atgagcaaac atcatttgaa gatgctaaag aaactataaa agaccaactg ttgaaaaata
    58021 aatatcaaga acaaatagaa aaattaacta aagaagctaa ggtagaaaaa gatgaaaaag
    58081 taataaataa aatcacaatc taagacttaa aaaaagagta atatgcttaa aaaagctata
    58141 tctggataag atatagcttt ttttgtatat ttttcttaac aatggtaaaa ataaacttaa
    58201 agtaatttat ggagggatta aaaaatatga aagcaacagg tatagttaga agaatagatg
    58261 atcttggaag ggtagttatc ccaaaagaga taagaaagac attaagaatt agagaaggag
    58321 accctttaga gatattcaca gcaaaagatg gagaagtcat acttaaaaaa tactctccaa
    58381 taggtgaatt aaatgaattt tcgcaagaat atgctgaaac attagctgaa gtactaggat
    58441 atggagtgtt gataacagac ttagattcta taatagctgt gtctaagctt cctaaaaaag
    58501 attataaaga aaaaagtata agtgatgagt tagaagcaat aataagtgaa agaacagtaa
    58561 aatattcaaa agataaaaac attttaccat tattcaagga agaccaaaaa gaatatacta
    58621 gtcaagttat catgcctata attagtgcat ctggtgactg tataggttca attgtagttg
    58681 tttcaaagga taaagataca attgcagaaa gtgaagaaaa atctttaaaa attgctgcta
    58741 actttttagg aaaacaagtt caataatttt atcataaaga ccttttacat gaaggtcttt
    58801 tgaagttttt gtgtaaaata ttgaatatag cattaaaaat atggtatatt aaagtttgtt
    58861 atataaacag tatggaggtt atgttatgaa caataacaaa aataaagact cctttctaaa
    58921 aggagccctt atacttgggc tagcaggtgt aatcgtaaaa ataatgggtg gattttttag
    58981 gattcccctt ggaaacatga ttggttcaga aggaatggga tactatcagg ctgcttatcc
    59041 tgtgtatact ctatttttaa cattagctac tgctggattt ccaacagctg tggcaaaatt
    59101 ggtttcggaa aaagttgcaa ttggaaattt taaaggtgca aataagatat ttaaggtatc
    59161 acatacagtt ttattcatta ccggtataat atcattttgt atattatttt ttggagccga
    59221 ctacatagta acaaacgtaa tgaaaaatcc aggtgcgtta tactctatga aagccattgc
    59281 tccagcatta ctatttgttc ctgcaatgtc tgcatataga ggatattttc aagggagaca
    59341 agatatgact aagattgctg tgtctcaagt tgcagagcaa ttttttagag ttgtattagg
    59401 tcttactttg gcttacttgc ttatgaagag tctaggtcag acttttggtg cagcaggtgc
    59461 tatatcaggg gctacaatag gttcaatagc atctatgctt tatttggtgt ttgcttatat
    59521 gctagggaga aaagagcgta gagcagagat agatgcaagc caaagattta aagatgaaag
    59581 agtgtcttat atttttaaaa agcttttaac tgttgccatt cctataacaa taggagcatc
    59641 agtaatgcca cttgtaaata tgatagataa tgttattgta ataagaagac ttatggaagc
    59701 aggctttaca tacaaagtgg caaattccat gtttggtcaa ttaacaggta tggcaatggc
    59761 cacaataaat ttaccagcag tcataacaac tgcaatgagt atgagcttag ttccagcaat
    59821 ttctaaagca tatgcattag gaaataagtc aaaagctaga aaagatacta agagtgcagt
    59881 taaggtaact ttacttatag tattgccttg tgcgtttggt atggcttctt tagcaatacc
    59941 aataatgggt cttttatttc cacatgaacc atcttctgta ggaactattt tgtttactct
    60001 tactccatgt gtattatttt taggattaat acagacattg actggtataa ttcagggaat
    60061 gggaaaacca atagtacctg taattgcttt atgtgttggt atgctatgta aaattgtaat
    60121 aagttatact ttaacaggta taccagatat taatgtactg ggttcagcat ttggtacagt
    60181 tacagcatac tttgtggcag ccatgattaa cttactatat gttaaaaaac acatgaatgt
    60241 aaacttttct aaaaaagaat ttataataaa gccatttata acagttatga caatgtttat
    60301 aatggttaag ttgagttatg gtgctttagt tggattctta ggaaacagca tatctacaat
    60361 aattgcaata tgtgttggtg gaattgtata tgtagtagta atacttggaa taggtggaat
    60421 taaaaaagaa gagattctaa ctatgccaaa aggtgataaa ttatataaat tgttaaaaaa
    60481 agttaaactt ataagataat ataggaatat atatgcaaat aaaggaaata ctatacaaat
    60541 agtaaatctc cttatttgta taataatata aaaataaagt gaaatttgct aataggagtg
    60601 atgttatggg taagataagt attgtaggtc ttggaccagg agactattct cttataagcc
    60661 aaggtgtgtt agaatcttta ataaatagcc ctaaagtttt ttttagaaca gagaaacatc
    60721 caataattga taaacttaaa gaaactatac aatacacttc tttagactac ttttatgaaa
    60781 aagaggaaaa ttttgataat gtttatttga aaatatctga atttatcata gaagaatcag
    60841 caaagggtga cctagtgtat gctgttcctg gtcatccaag agttgcagag aagactgtaa
    60901 gtataataga gagattagcc aatgaaaagc acatagcagt agaaatattg ccttctatga
    60961 gctttgtaga cgctatgttt aactatttgg ctatagaccc atctgaggga tttaaactgg
    61021 tagatgcttt tgaaatagaa aattcatata tagacttgga tacaagtatg ataataactc
    61081 aggtatatga taaatttata gcatctaatg taaagttaaa acttatggaa cattatgact
    61141 ataatcaaga agtatgtata gtaaatggag ctggtgtcaa agatttagaa agtaaaaagt
    61201 ttataaagct ttgtgaatta gacagaaatg aaaacttttt tgactattta actagcttat
    61261 atatacccaa aagttctaaa aagatgtata atacagtaca tgacttagaa ataatagtta
    61321 atacacttag aagtccatct ggatgtgaat gggataaaaa acaaacacat caatcattaa
    61381 aaaattctgt tatagaggaa gcatatgaac tttgtaatgc aattgataat aatgatatag
    61441 atgaaatggt agaagagtta ggtgatgttc ttttacaggt tatattccat tgtcagatag
    61501 gaaatgagga gggctacttt gatttaaaag aagtagtaaa tggcatatgt aaaaaattaa
    61561 ttcatagaca tcctcatata tttagtggtg caaaattaga tatgtctaag tttgagaaaa
    61621 cttgggaaga attaaagaaa gaagagaaag gcgaaagtac tataacagat ggccttagaa
    61681 ggataccaaa acatttacca gctcttatga aggctagtaa agttcaacat aaagcagcac
    61741 ttgtcggctt tgattgggac aatgtagaag atgtatttaa aaaaattgaa gaagaatatc
    61801 aggaattact tgacgaacac agggtcggga atataaaata cataaaggaa gagctaggcg
    61861 atttattatt ttcgatagta aatctagcta gatttttaga catagactca ggagaagcct
    61921 tgaactgtac tacagaaaaa ttcataaata gatttgactt tatagaaaaa agtgcaatga
    61981 acttgaataa aaaattagaa gacatgacac ttgaagagat ggatgagttt tggaatcagg
    62041 ctaaaaaaca ataaaattgt ggaaggaaat ttgtcaaaaa tctagaactt ttatcacaaa
    62101 gatagaagtc tatattataa acatttttag gaggtaaata tcgtgaataa agctgaatta
    62161 gtatcaaaga tggcagaaaa aagtggatta acaaagaagg aagcagaagc tgcgttaaac
    62221 gcatttatga gttctgttca agatgcacta gtaaataatg aaaaagttca attagttgga
    62281 tttggaacat ttgagacaag agaaagagct gctagacaag gaagaaatcc aagagatcca
    62341 gagcaagtta tagatatacc agcttctaaa gcaccagttt tcaaagctgg aaaaggatta
    62401 aaggatataa taaatggata attagaaatc ttgtgaaaaa gtgtggataa atttgccaca
    62461 ctttttctat agaaccaagg aaggtgataa ttatgagatt agataaattt ttaaaagtat
    62521 ctaggattat aaaaagaaga actgttgcta aagatgcatg tgataaaggg attgtcacta
    62581 taaatggaaa agttgctaaa tcttcttcag aggtgaatat tggagataca ttagaaatac
    62641 aatttggaga gaagaagatg aagtttaaga taaatgaagt aaaagagcat gtattaaaaa
    62701 atgatgcaaa agaaatgtac aaaattttag aataaaatta atagtcctct agtatattaa
    62761 tagtagtatt attgaaatat ttactggggg gatattatgg aacagaacat aactttgaaa
    62821 gatagatcaa agctggttat ttcgggagtc gagcacatat actcttttaa tgacaaaaga
    62881 gtagagctca aaacttctgt aggcgagatg gtaattgaag gcgaaaacct agatatgagc
    62941 aagctaagta tagacgaaaa tataattagc atagatggaa ctataaattc tatggtatat
    63001 gcaaagccta aaaagccaca agagagtttc ttgaaaaagg tatttagata gatgatacct
    63061 tttactcaag atgtaagcat tttttatgct actatttatg gtggaatact cataggggtg
    63121 ttgtttgatt tttatagagg gctaagaggg aattttaaat tcatcaatta ttttgctata
    63181 atctttgatg tattgttttg gtttctagct acggttataa tattcgtaac aataaacctt
    63241 acagaatttt ttgatttaag atattatcat tttgtggctt tgtttattgg ttttatttta
    63301 tactataaca ccataagtaa aattgtttta tcaattatca ataaaataat tcgttttgtg
    63361 agaaattctt ttaaaaaagt tacacattac atagtcagtt ttttaaataa tttgtattac
    63421 gttataatct attcactaca cctactattt gatattatat tttatatacc aaacatattt
    63481 atagcaacaa gaaaaagtat aaaaagaaaa tcaaataaaa aattaaagaa caagaaaaaa
    63541 tcaaaaccta agaagaaaaa aaataaaact aaaaaaaggg tgtaggaaaa atgaacttaa
    63601 gaaaaaaatt ttcaggacag attattgtta tcagtttatt tttaggaata tcaattttta
    63661 gtatgatgac aggatttgta tttgagtata caaaagctaa ggaatataaa aaagaaatag
    63721 caagtctaaa taaacaatta aaaaagactg aaatacaaat aaacagctta aagaaagatg
    63781 aaaaatcata tgaaggagac ttagaggata tagcaagaaa aagattgaat atggtaaaac
    63841 caaatgaaac agtttatgta gacataaatc gttgattaaa acttgctaat aagtttacag
    63901 atgtattgat tttaaaattt aagatttaat aaaaaagtca ttcagttaag gatggctttt
    63961 tgtttttatt gaggagggaa tttcataatg aaagtaggag ctatagatat tgggacaaac
    64021 tcaatgagat tattgatagc agattataga gatgggaatt ttataaacag aaaaaaattt
    64081 gttaatacaa ctagaattgg ttatggtgtg gatagtaaag gatatataaa agaagatgct
    64141 atcaaaagaa atataaaagc attaaaagaa ttttctgata agtgtaatga tgaaaagtgt
    64201 gaaaaaattt attgtatggg gacatctgct cttagagaca gtaaaaatgg agattacttt
    64261 gttaaattag ctaaagaaga aactggaata gatgttgata tagttacggg aatagaagaa
    64321 tcaaatttgg gctttaaggg tgttttagaa ggaatacaag gtgatgatac tattattgtt
    64381 atagatattg gaggaggttc aacagaattt attgtaggtg attcagaagg aattaaattt
    64441 aataaaagtg agaatgtagg tgctcttagg atgacagaga ggtttttgaa gcacgatatt
    64501 attgaagatg aagagtttga agctatgagt tcatttatat atgatgagat tgacaaaacg
    64561 ttaaatcata taaaagacag aggattaaaa actcttatag gtataggagg tacgattaca
    64621 tcattatcag caatgaatca agaattagag atttactcta tggaaaaaat acataatagt
    64681 aaaatacaca aaaaagaaat agaagaaatt ctacaaaaat taataaagat gacattaagt
    64741 gataaaaaga acttaaaagg gctacaagaa aaacgtgcgg atattataac tgctggtgta
    64801 aaaatattga atataataat ggaaaaatta gaaatagaag aaataaccgt aagtgaatac
    64861 gataatttgg aaggcttaat atgtcaaaag tcaaaaaaaa tgtattaata ttttttgatt
    64921 tttaaaaccg attatgacaa aaaaaattac tcctctttgt tacaattctt aatataaaca
    64981 aaagaggagg tttttttatg caaagaagtg tagcagttgt gaagaataag actgtaaaac
    65041 caaatgtcat aaaattaata agcggatata ttgattttac tacggcggca ttcgctgtga
    65101 tgggattttt acttagtagg tctattatag tagactctgt agctccttta ggaattgcct
    65161 tttttatatg tgcatcaaag attgataagt acaagatacc tgtattcata tcaacattat
    65221 taggaatatt gttatcatct aatgattttg gaaatgtagc aaaatatgct gtgtgcctta
    65281 caatatttat ggctatgaat aaaaaattaa aattatcaga ttcaattgct aaggttgcat
    65341 ttataggtgc aataatacta cttccaatct ccatgggaca agctttcttt tcaaataggt
    65401 atgtatatgg tcttcttata gcaggagtag aagcaataat aatgtttata tctatatatg
    65461 tgttttctta tgggataggt ttaatggcca atgtcaacaa taggatgtct gtacggacag
    65521 aagaagccat atcaataagt ttactgctaa cctttagcat aatgggaata ggagatatat
    65581 ctctgtttgg aatatcaata aggaccattt tatcgaccgt attgatattg ctgacctcaa
    65641 tagtaggtgg agctaccatg ggagcatcta gtggtgtaat tattggtgta gcatccattt
    65701 taaataatat gacatcagct gtttatatgg ggatatattc tttttcagga ttaataagtg
    65761 gagcatttaa taaaataaat aaatatttct gtatactagg atatattcta agttggacaa
    65821 taatatacct atatacttct ggtattactt ctaatatgat gcaattaaga gatatactat
    65881 taggttgttt gatagtacta gtattgccag agagattatt taataagatt gaaaaattaa
    65941 taaaaagtaa tgtggcttca aatgaaatcg tatacgatta tattatgaga agcaaaaacc
    66001 taactaatag cagattgaac agtatttata aaacttatga tgaccttgca gatacatttg
    66061 ataagataag agaaaaagac aaagtgctag accaaagaga catagctaat gtaatagata
    66121 tgattcataa tgatgaatgt aaaagttgta gtatgagaag aatgtgctgg gagtcaagat
    66181 ttaatcatac atacactatg atatataaca ttcttgaaaa aattgaggag aaaggggagt
    66241 taagtctaaa cgatatacct aaaaatttta gaaaggaatg tatgaaacca gagtcaatag
    66301 tgaaaatatc aaatcactat tacaaaatgt ttgtattaga ttatgattgg agtgtgaagt
    66361 tttcagaaag tagaaagtta atagcaaatc agataagaag tatttcaaaa tccattaaat
    66421 ccttatctca agatttagaa ggcgacataa tgctagatat agaaaaggaa aaaaatatat
    66481 atgaacagtt agaaagatat gatataacag ttgataaagt aagttactta acaaaaagta
    66541 acagtgagtt tgaaatatca atagaaaaga aaacttgcca tgatggatgt atgtgtgaag
    66601 ataaaattgt aaatataatt tcagacctag taggggaaaa tatgtctgtt agaaaaatag
    66661 gatgtcactg tttaggaggt aaatgcaagg caacattcgt taaatctcaa aaatataagg
    66721 ctgttacaga agtttcagca atgtcaaggg atgggcatat cctttgtgga gacaattaca
    66781 catacatgga gataaatgat ggtaaataca tgatggctat aagtgatggt atgggaaagg
    66841 gtaaaaaagc atatgaagaa tcttctgcca ctattgatat actggaaaaa atgatagatg
    66901 caaaaattaa agatgagata gtcattgata ctataaacaa tatgttgttg ttaaaatctt
    66961 cagaagagat gttttcaact ctggatttgg gaattttaga tttaaaacga ggttgtttag
    67021 aaactataaa gatgggagcg tgttctactt atataaaaag agaggatgga gaagttgact
    67081 taatatcatc atcatcatta cctgtgggta tattatcaga tgtaaaaatt gatagaaaaa
    67141 atgtaaaagt taaggaagga gactatgtta taatggtttc tgatggaatt gtagatgcag
    67201 gaagaaataa caatttagga gacaattggt taatatactt cttaaagaat atagagacta
    67261 caaatccaaa ggaaatttca aatttaatat tggatagagc attagagtta caagcgttac
    67321 aaatcgaaga tgacatgact gttttagtta cgaaaatatg tacaaaataa ggtttacaag
    67381 attatgtgta taagatgtgg ataacttgtg tataatatgt taatagactt ttgaaaaaag
    67441 tatagttatt aacaaaaata acatataaat caacaaaatt tgttgataag tatggcagaa
    67501 atgttaataa aatgtaaaaa aagaaaaaac aaaagctatt atcaaaagag attcatgttg
    67561 aaataactaa atttctaagt aaagaaaacc tgtgaatatg ttgattatta tgtggataac
    67621 tattaattcg atatttattt atatagtatg aataattgaa taatatatct aaaaaaagcc
    67681 atcctattat taagtaaact tattgaaagg atggcttttt atgaatagaa aattgtgcaa
    67741 agcaataata cctttattta tttctatttt ttgcatgtta ataataagta taaaagctga
    67801 ccaaggatat caaaagaata atgacagagc aattactgtc atgaataata atgatactga
    67861 agatgatgct gaaaaaaaac aagaaataga ctatgaaaaa acatgttgga atttgcaatc
    67921 tttgtttaaa agtgatgacc aatggaaaaa agaacttaag agttttgata aagatactaa
    67981 agagttgaaa aattacatag gtaaagtaac aaaatcgcca acacatttgt cttttgcctt
    68041 gaacattaaa gaaaaacttg atataagact aaataaactt tctgcctatg taaaattaaa
    68101 acaagatact aataaaaatt cgtataagta cttagatatg aatgatagta tgagtaaatc
    68161 ctatgggaat tatatgggga tttgttcaga tttagagtta gaaatactaa aattatcaaa
    68221 caaagattac aagaaaatta tttcaaataa aaatataaat aggaaatatg gtgcatattt
    68281 aagagatata agaaggaata aagtacatta tttggatgat aaatcagaag atattctaag
    68341 caaagtatct tcaataagta gtcttccaag tcaagtgtat gaattattta gaaatatgga
    68401 tagaaaaact aatttaacac cagctcaata tgcgagtgaa ttagagtcat cagatagaga
    68461 aaatagaaaa aaagcttatc aagatgaact tataacttac aatgataata taaatacaat
    68521 tgcaggttta attactggtc aagttaaaaa gaatattttt tattctcagg aaagaggtta
    68581 tgagtcttct ttagaaatgt acctggaagc tgataatgta gatgaaaaag tatacaataa
    68641 tttgataagg actgtaaata gtaatatgga cagtctacac aaatatatag atttaagaaa
    68701 aaaagtatta aaattagata aggttcattc atatgatatg tttgtgccta tggttaaggc
    68761 tgttgataat aatattaatt atgaaaaggc acaaagtata gtatattcag cgttgtcacc
    68821 tttaggaaaa gaatacaatg atgtagtata taaagctttc aatgaaaaat ggatagatgt
    68881 ttattcacat gaaaataaag taagtggtgg atactgctta tctgtttatg aaaatcatcc
    68941 atatatactt ttaaattata ataattcatt aggttcagtt tcgactttat cacatgagct
    69001 tggacatgct gtatatgaat acttaagttc taagaatcag aattatttta attcaaatcc
    69061 gtctatattt actcacgaag ttgcatctac aacgaatgaa gcattacttt acgagatgct
    69121 tataaaagaa gctaaaggca atgatgaaaa agcttattat ataacacagt atttagactt
    69181 gataaaagat actctttata cacaaactat gtatgctgaa tttgaaaaaa ctatacacga
    69241 gttagtagaa aaaggaaaaa gtgtaaacgc attagtatta aatgatgttt ggggacaact
    69301 tcttaaaaaa tattatggac aaagctatga attagaccaa ttatctaagg tcggatgggc
    69361 tagaatccca catttttata atagtttcta tgtctataaa tatgctacag gttgtagtgc
    69421 aggagtaagt tttgcccaag acatcctaaa gaatggttca gataactata tgagctttct
    69481 taaaaaggga agttcagatt atccaataaa cttgttaaaa tcaagtggca taaatttaac
    69541 taatacaaaa cccatagaaa atacaatcaa aaaatttgat agtttagtag cagaacttga
    69601 aaagctatta taaacattat tgtaatccac atggtaaata tatcgaatta atgataaaat
    69661 atactaaaag tttaaagtcc tatcaagata ggacttttta tttattaatt agtgggagat
    69721 gattctatgc aagttaaagt taaaaaggct gtaataccag cagcaggttt aggaactaga
    69781 tttttgccag ctacaaaggc acaaccaaaa gagatgttac caatagttga taagccaact
    69841 ctacaataca taatagaaga agctgtggcc tcaggaatag aggaaatact aataataaca
    69901 ggtagaaata aaaaatctat tgaggaccac tttgataaat ctgtagaatt ggaacttgat
    69961 ttagagaaaa aaggtaagaa agaattgttg gaggtagtac aaaacatatc taacatgatt
    70021 aacatacatt atataagaca aaaagagcca aaagggttgg gagatgcaat ttattgtgcg
    70081 aggcatttta taggtgatga gccgtttgca gtaatgttag gggatgatat tgtagataat
    70141 gatgtacctt gtctaaaaca actaacagat gcatatgagg aatatagaac tacaatattg
    70201 ggtgttcaaa aggtaaatca agaagatact aataaatatg ggataataga agcaaaaaat
    70261 atagaaggta gagtttacaa ggtaaaagac atggtagaaa agccagagtc aggaaaagct
    70321 ccttcaaata ttgctatttt aggtagatat ataataactc ctgaaatctt tgatattctt
    70381 aaggatttac caccaggtaa aggtggagag gtacaactta cagatgcttt aaaaatatta
    70441 tctaagaaag aagctatgta tgcatacaat tttgaaggca agagatatga tgttggggac
    70501 aagctaggat ttttagaagc tactgttgac tttgctctaa agaaagaaga ccttaaggaa
    70561 gattttataa aatatttgaa acatgtatgt agtgaatttg ataaaaataa caatgtgtta
    70621 tacaataata cagatgaaaa aatagattta attgaagtca atgaagctag agaacttgaa
    70681 attcaaaaat aattaatgaa aggtgatgta tggtggcaaa atcaagtcag aaaagaatta
    70741 atagaataat agctgttggg attgtgggaa tcctcgtaat aacgagtata acagctgttt
    70801 tgagtatgat tgcaactatg taataagata aatgacagca gttgatatac ataaatataa
    70861 aaaccatata taacttgatg tagtcaaaaa cttacaatat tttagttata tatggttttt
    70921 agaatttata aatgtaaaaa aaaattttta taatagttgc atattttaaa ggaaaatatt
    70981 attataagat ataaatatag tgataaattt ataaaaacgt gctaagattg gacttgaaat
    71041 acagtaatat agtaggtaat aatgttttaa attttattta ttgaccataa tatataaata
    71101 atgatattat tagacttaac tgtttaaata aaacttttct ttttcatgcc ttcaatttcc
    71161 aaaataagta tgtatataaa tgaatatata gatgaggagg atttttttga ttttagatga
    71221 taaagtatta gaaggattag gtagtaagac cttagttgag ctacgagaga tagctaaaga
    71281 attaaaaata aaatcaatta ctacatataa gaaaaatgag ttaattgaga ttataaattc
    71341 caaaagtaaa aatgaggaaa aagtaaacga agagactaaa gaaaaaaatg aagatattaa
    71401 agaagacata aagaatagaa aagaaaatat agagcgtgaa agtatagaaa aagaagttga
    71461 atataagagc cctaccaaat catacaacaa gaaagaaata gatacccaaa atatttccca
    71521 aaaccaagaa catagaaatc aaagaaatga ctataataag acaacgagtg taaatgatag
    71581 taataagtca tcaaatatat ctaatagaat ggtcagaaat aataataaaa actattatat
    71641 gcctaaacaa gttgatgaat ctaagatagt agatgaattt aacacatcaa aagaggatga
    71701 agtagtaggt gtacttgaaa ttctgccaga tggatttggt tttttaagag gttcaaatta
    71761 tttgtctact gaaggagatg tttacgtttc accatctcaa ataagaagat ttaatatgaa
    71821 aacaggagac aagattaagg gaataactag acatccaaaa agtggagaga aatttagagc
    71881 acttttatat gttcaaaaaa taaatgatga aaaccctgac acagctatac aaagaaatgc
    71941 gtttgagaca ttaacaccaa tatttccaga agaacgactt acactggaaa ctaatagaaa
    72001 tgaaatagct acaagaatta tagatttaat ttcacctata ggaaaaggac aaagaggatt
    72061 gatagtagca cctccaaaag ctgggaaaac agttctttta aaaagcgttg caaatagcat
    72121 agctaaaaat catccaaatg ttgaacttat agtactcctt atagatgaaa gaccagagga
    72181 agttactgat atgaaagaat ctattgaagg agacgttata tattctactt ttgaccaagt
    72241 atcaagtcat catgtcaagg ttgcagagat ggtgttaaat agagctcaaa gattagttga
    72301 acatggtaaa gatgtagtaa ttttactaga tagtataact agattggcaa gggcatataa
    72361 tcttacaata tctcctacag gtagaacttt atctggaggt attgaccctg gtgcacttca
    72421 tggacctaaa aagttctttg gtgcagcaag gaatattaga cagggtggtt ctttgacaat
    72481 attaggaaca gcattggttg aaactggttc taggatggat gatgtaatat ttgaggaatt
    72541 taaaggtaca ggaaatatgg agctacattt agatagaaaa ttagcagaaa aaagaatatt
    72601 ccctgcaata gatatttaca agtctggtac aagaagagat gatttattgc ttgatgatga
    72661 agaaaagact gctctttgga gattaagaag agaaatgagt aataattcag taatggaaat
    72721 tacagataaa gtcattgaac ttataaagag aactaaggac aataaagagt ttgtaaaatc
    72781 aataaagaat ttgtaaaatc aataatacaa atgcaaatat ggattatata aactaataga
    72841 aaaatcactt gaatggcata ataagttgtg ctataataat gtagttgata atgtaaaaag
    72901 aaagttaata taattaattt ttttaaataa agagaagagg tgactataat gcaaaaggaa
    72961 atacaaccaa aatacaatcc agttgaagtg cgttgtgctt gtgggaacac atttgttgct
    73021 ggttcaacta aggacgaaat aaaagttgaa atatgttcag agtgccatcc attctataca
    73081 ggaaaacaaa agaacataga aaaaggtgga agaatcgaca aattcaagaa aagattcaaa
    73141 atggactaat ttttataaaa gtggagttgg aggaagccaa ctcctatttt attacaaaaa
    73201 aacattcata ttctacttta aattgtcttt ttgtattata ataataaata tgtaaaattg
    73261 agtattctaa atacaataaa atgaggtgag tggatggcaa aacaagctgt aggtgggcaa
    73321 gctgttattg aaggtgtaat gatgcagtca aaagacaaaa gagcggtagc tgttagaaaa
    73381 agtgacggag aaatagcact aaaagaagat agaataaaaa gttgggtaag agataagaat
    73441 atagataaga taccttttgt tagaggttct tttgttatga tagatacaat gattcaaggc
    73501 ataaaaagct taaacttttc ttcagagttt tttatggaag aagctgaaga ggataaattt
    73561 gatttattta taaagaaaat ttttaaagat aaggcaaatg atataattat aatattttca
    73621 ttggtcatag caatgttact ttctgctgga ttattcattt ttatacctac attggtagga
    73681 ggagcttttt ctaaagtaat gcctaatgat tttatgctta atttaataga aggaatcata
    73741 aggatagcta tattatttgc atatatagta ttgatttcac gaagtaagga tatagaaaga
    73801 gtctttcaat atcatggagc agaacataaa tctatatatt gctatgaaaa tgatttagaa
    73861 cttacagtag agaatgctag aaaatttaaa agattacatc ctagatgtgg tacaaatttc
    73921 ctatttatag ttatggctgt atcaataata ttgtttgcat tttttggttg gccaaatcca
    73981 attcttagaa tatttatgag aattatatgt gtgcctatag tagcaggttt atcttatgag
    74041 gtaattagag ttcttgggaa atatgataat ggatttacta aaattattgc atatccagga
    74101 atgatgttgc aatattttac aacaaaagag cctgatgatg agcaattaga agttgctttg
    74161 gaagcattga aagcagtagt agattagaga aaaggatatt tgttatgacg ataaaagata
    74221 taattattaa atattcagat aaactaaaag atataagtga tactccaaga ttagatacag
    74281 agttattgtt acaaaaaaca ttgggtgttg atagattata tattcactta aatctgaata
    74341 aagagctaac tgaagaacaa aaaacaaaat ttatgggttt tgcagaagaa agattaaatg
    74401 gaagaccaat agcatatata gttgaaaata gggagtttat gggattagat ttttttgtta
    74461 aggaaggcgt attgattcca agaccagata cagaaacttt agtagaagaa ataattgaaa
    74521 tatgtagaga aaaaaaagat gtaagtatat tagatatagg aactggttct ggagctatta
    74581 caataagttt agcaaagtac attgaaaatt ctaaaatcat gtcttttgat atatcagaga
    74641 cagcattaga aatagctaaa aaaaatgcta ttataaatga agttggtgaa aaaataaagt
    74701 atattaattc tgatttattt acagcaataa gtgatagtaa cataaaattc gatatcatag
    74761 tttcaaatcc accatatatt aaaaaacaag atatagaaac tctgcacaaa caagttaaag
    74821 attatgaacc atataatgct cttgaaggtg gagaagatgg attagacttt tatagaagaa
    74881 taacagaaca aggtaagaaa tatttaaata agtgtggaat attagcatat gaagttggtc
    74941 ataatcaagc tgaagatgta ataaatatta tgaaaagtaa tggatataaa aaaatataca
    75001 caaagaagga tattcaaggc attgatagag ttgttatagg ttacaatata tgatataatc
    75061 aacaattgat atgttacatt taatcgaggt gaaagtatgt taaaaaaatt agaggtattg
    75121 gaagatacat ataaagactt aagtgaaaaa ataggtgatc cggatgtaat aaatgatcaa
    75181 aaagtttggc agaaatatat aaaagaacat gctgacttgg aacctattgt tatgaaatac
    75241 agagagtata aaagcgtttt agatagtata aaagaatcaa aagaaatttt acaagaagag
    75301 tcagatgaag aattaagaga attagctaaa atggagttag ctgagatgga agaaaaagta
    75361 gcacctctgg aggaagaaat aaaaatacta ttattgccta aagaccctaa tgatgataag
    75421 aacgttatag ttgaaattag aggtggagct ggaggagatg aagcagctct atttgctggt
    75481 gatttattta gaatgtactc aaggtatgct gaaagaagaa gatggaaaat agaattattg
    75541 agtgcaagtg atactggagt tggtggatat aaagaagtat cttttatgat aaaaggtaaa
    75601 ggtgcttatt caagattaaa gtatgaatct ggagttcata gagttcaaag aataccatca
    75661 actgaatctg gtggaagaat acacacatca acttctactg ttgcagtatt accagaagtt
    75721 gaagatgtag aggttgaaat aaatccaaat gatttaagaa tagatgtttt ccgttcttca
    75781 ggtaatggag gacaaagtgt caatactact gactctgctg ttagggtaac acatatacct
    75841 acaggtgaag tagtatcttg tcaagatgga aaatcacaat taaaaaataa agagcaagct
    75901 ttaaaaatat taaaagctag actttacgat aaagcattgg cagaacaaca caaagatata
    75961 gcagcagaaa gaaaaagcca agtcggaaca ggagacaggt cagaaagaat aagaacatat
    76021 aatttccctc aaggtagaat aagtgaccat agaataaatt taactttata taagttagat
    76081 gcattcttag atggagatat agatgaaatg atagatgctt taattactgt tgaccaaaca
    76141 gaaaaaatga ctgcaatata agtaatgttt taaagaagct gtttcttcct agaaattatt
    76201 ttctattttg aaacagctat tttgcatttt aggtcatgtt atcatattct attgtatata
    76261 attgttatat acaagtatag gagggttata tgatactaaa agtaacaata attgggcttt
    76321 tggcaggtgt tatagggaca ggattaggag gagtaatatc agccattttt aaaagagagg
    76381 tcgataaata tctaagtttt tttatgggat tatctggtgg aataatgttg gctgtagtgg
    76441 tgtttgattt aatgaaagaa tccatggaca aaatggggat aatcaataca gttatattta
    76501 cgtttgttgg agctctcata actatgtaca taaaaacaaa attagatgtg tctggaaata
    76561 tggcatctgg atatcttata ttcataagta tattactaca taatttacca gaaggactag
    76621 ccattggttc ttcttttatg tcaactgaaa gtttaggtat tacacttgct atagttatag
    76681 gtctacataa tataccagaa gggttagcta tggcattagg cttagtttgc aataaaatga
    76741 aactaagtaa agttattttg tttactgtaa tagctggatt acctatgggt ctaggaagtt
    76801 ttttaggtgt ttattttgga ggagtattta cttctcttat aggagtattt cttgctacag
    76861 caggaggtac tatgatgtat gttgtattgg aagaaatttt tccacattca aaaagtgtgt
    76921 attgtataat tggtttcttg ttaggtacaa tgattgtaaa ttatatataa aattaaaatg
    76981 aaagcaggca tacaaaagat gaaaactatt ataagtaata ttgatataaa taatattgat
    77041 tatgaggaaa taaaaataca agcaaaactt cttagagaag gtaaaacagt tatttttcca
    77101 actgagacag tatatgggtt gggtgcaaat gcattagatg aaaatgcagt aaagaagatt
    77161 tatgaagcta aaggaagacc tagtgacaat ccattgatag tccatatata tgaaaaagaa
    77221 gaagtatatg acttagccaa agatataagt gataaagcta aacttgttat agaaaagctt
    77281 tggccaggac ctataacaat tatattaaat aaaaaagata taattccata caaaactagt
    77341 ggagggcttg atacagttgc aataaggatg ccttctaatg ttattgccag ggcaataata
    77401 aaagaagctg gtatacctat tgcagctcct tctgcaaata tctcaggtcg cccatctcca
    77461 acaaaagcaa aacatgtata tgaagagatg aatggaagag tggatggaat agttttagga
    77521 ggagacagta attttggatt ggagtcaact gtattggatt taacagaaga gactcctatg
    77581 atactgagac caggaagtat aactaaagaa gttttagagg aacttcttgg agaagtaaag
    77641 cttgacccat cattaagtaa aaaagaagat aatcaaaaag ctaaagctcc tggaatgaaa
    77701 tataaacatt attctccaaa tgcagatgta tatataatat ctggaaaaag agaaaatgta
    77761 gcaacaaaga ttaatgatat gataaaatct aatagagaca aaggtctaaa gacaggtgta
    77821 atgtgtatta gtagaaaccg agagttttat gatggagaag taatcgattt aggaaactct
    77881 cttgaagaag ttgcaagtaa tctgtttgat gctcttatag aaatggacaa aaagggtgtt
    77941 gatgtcatat attcagaaga gttccctaag actggagttg gacaagcaat tatgaataga
    78001 ctactgaagt cagcaggata caaagtgata aaggcttaag agggagttgt aaaatatgaa
    78061 tatacttatt gtgtgtacag gtaatacttg tagaagtcct atggctgagg ctatactaag
    78121 gaaagcaatt aaagaatctg gaagaagtat tgaagaatat tctatatcat cagcaggtat
    78181 atctacagca aatggaatgg gtgcttctga aaattctata gaagtactaa aagaaatcgg
    78241 tatagactta tcaaatcata gaagtaaagt aattactaaa aaattaattg atgaatcaga
    78301 tataatacta acaatgacta aatctcataa agaaatttta gtacaagcag ttccaaaatg
    78361 caaagaaaaa gtgtatactt ttaaagggtt tgcaaataaa aatgaggaag atatatctga
    78421 cccatttggt ggaaatttgg atatttatag aagcactatg agagaaataa tgtattctgt
    78481 caacgaaata gtaaaaaaaa tatgaaacgg aggataaaca aatgaagata ggattaggct
    78541 gtgaccatgg aggatataat ttaaaaaaag aaataataag ctatttagaa agtaaaggca
    78601 tagaatgtgt agattatgga actaataatg ctacagattc tgttgattat cctgtttatg
    78661 gagaaatagt tgcaaattca gttataaata aagaagtaga ttatggaata ttatgttgtg
    78721 gtacagggat aggaatatct ttagctgcta ataaggttcc tgggataaga tgtgcagttg
    78781 tatcagatgt attttctgca aaaatgtcaa aagcacataa tgatgcaaat atgttatcat
    78841 taggcgaaag agttttagga aaaggtctag cacttgagat agttgaagca tggattaata
    78901 cggattttga aggtgataga catgctagaa gagtaaacat gattaaatca atagaagaaa
    78961 aacataataa ataataaaaa ttacagtttt atcaggagga attatcaaaa tgagcaaagt
    79021 ggtagagaca aaccatccat taatacaaca taaattaact ttaatgagag ataaaaatac
    79081 tggttctaaa gactttagag agcttttaac tgaaattgca atgttaatgg ggtatgaaat
    79141 aacaaaagat ataccattaa aagatgtaga aatagaaact cctattcaaa aaacatcatc
    79201 taaagtagtt gcaggtaaaa aattagcaat aattccaatt ttaagagcag gtcttggaat
    79261 ggtagatgga ttagtgagct taatgccagc agcaaaagtt ggacatgtag gactatatag
    79321 agacccagaa acattaaaac cagttgaata ttattgtaag cttcctcaag acataggaga
    79381 aagagatata atagtagttg accctatgct tgcaacaggg ggttctgctg tagcagctat
    79441 agatttactt aagtctaaag gagctaaaag tataaaatta gctaatttag tagctgcacc
    79501 agaaggtata gcagaagtgc aaaaatatca tgatgatgtt gatatatatg ttgcatcagt
    79561 tgacgaaaga ttaaatgaac atggatatat aattcctggt cttggtgatg ctggagatag
    79621 attgtttgga actaaataaa aataaataac tataaatatt tattagggac tttcttatat
    79681 agtgtatata attttatgta agaaggttct tttttatata ttttttctca aaataggaat
    79741 tattgaattg ataaatttct cgaattgaga ataaaaagtt tttaccaatt aaaaaagatt
    79801 gcttatttta caaggtttta tagaatagta gtctatttta atgtggcatg ttagttgctt
    79861 acgattaaga ttataggtat taagtgtatt agttaaattg agaattgaat attaataata
    79921 ttattgggag tggtaataca tgaagaattg tatagtagta agaggggctg gagatttagc
    79981 aacagcagtt attcataaat tacacaacag tggttttaaa gtagttgcgt tagaaataga
    80041 agagccatta gctataagaa ggcaaatttc tttttgtgag gctgtttatg aaaaaaaagt
    80101 aactgttgaa ggtgtagaat gtatattttg caatactgaa aaagaaatag aagaagtttt
    80161 atctcaacat aaagtagcct tgattataga cccaaaagga gaaaaattat ctaaattaaa
    80221 tccaatcgtt ttggttgatg gaatactcgc taagaaaaac ttaggtacaa ctaaagatat
    80281 ggcaccactt acaatagctt taggtcctgg attttgtgca ggagaagatg tagatgttgt
    80341 tatagagaca atgagaggtc ataatctagg aagaataata actaatggta gagcagaaaa
    80401 aaatacagga gtaccaggag caataaaagg tttttctaaa gagagagtta tgcattcaca
    80461 gtatgatggc ataatggaaa atgtatgtaa gataggtgat atagttgaaa agggagacct
    80521 actttgctat attaataatt atgaaaaaaa acatgaagtt agagcaacaa tatctggagt
    80581 ccttagaggt ttattaaagg atggagcaaa ggtaaacaaa aatttaaaaa tactagacat
    80641 agaccctaga aaagaagaag ttaagaatgc ttttacaata tcagataaag ctagatgtat
    80701 agctggaggg gttttagaag ccatactaaa caatgggatt tatccccaaa attaatttat
    80761 attagataaa gctattaata ttttattaag atgatataat aaatttaata gattaaagag
    80821 agcatatgac gggtatttaa aatttaaatg gtaaaaatgc tcttttttta ttggacattc
    80881 aattaacatg tgtatataat ataaaaataa gtatttattt aggttgaaat ctaataaata
    80941 tataatacca gtacataagc aaagaggaga aaagtaaatt atgagaccat catgggatga
    81001 atacttcatg gaaattgcag aagtagtgaa aaagcgttca acttgtatta gaagacaagt
    81061 aggagctgta attgtaagag acaaacaaat attaacaact ggatataatg gttctccgag
    81121 aaatcttgaa cactgtgaaa atattggatg taagaggcag gaattaaata ttccctcagg
    81181 agaaaggcat gaattatgca gagcattaca tgctgaacaa aatgcgataa tacaagctgc
    81241 tcataatggt attagtgtag atggggcaac tttgtatgtt actacaagac cttgtgtttt
    81301 gtgtgcaaaa atgtgcataa atgcaggtat attgaaaatt gtttatgaag gtgattatcc
    81361 agatgatatg tctacagaat tattaaaaga agcaggaata gaactcataa aattttagtg
    81421 gataaaattt atatgacaat aaatgtcaaa ttatgacact gaaatacaat ggtatcaaat
    81481 gattaaaaga aagtatttga aaaatcatca gattagtagt aagatatatt ttataattaa
    81541 ataaatttat gagatagatt aattacatgt tatttatttt ataataatga gggatactat
    81601 ttatgagtaa aagaaataca catacagaaa ttgcaaagtt gtttagttta ataagccaaa
    81661 taggattgat gattgtaata tcaatattag gatgtacttt cttaggaaaa tttttagata
    81721 gtaaattgaa tactactcca gttctgacaa taatattttt attgttgggt gttggaggtg
    81781 cttttagtgg tgtttataaa actctaatag tatatacacg aaggaagtga tatattggat
    81841 ataaaattac ttaaccaggt aaggtcagta acaaaaggta tagtaatatt tgatgtagtt
    81901 ttaattgtgc taatgttgat aacatcaaca ttaagtaaac cagtattatt aggacttata
    81961 tttggctcta taatagctat gttaaatttt agattattgg cgatatcact ggaaaaatca
    82021 gtgacatttc ctgctgggaa ggcacaagct tatgcatcag cacaatatgc tgttagatta
    82081 tttataatgg cagttgttct aattgcatca gctacagtac cacatttaaa tataatcgga
    82141 acagcattgg gattattgag cacaaagttt gtcatattat caaaaaactt tataaataaa
    82201 ttaaagagga aggaggcata agtatgaatc aagcgcatta ttatctcata ggaaacttta
    82261 agattagtga aacaatacta gtaagttggg gaataatggc ctttttagcg atagcatctt
    82321 attttttgac tagaaactta aaaaaagttc caactagtaa agttcaaata ttcttagaat
    82381 ttgcagtagg tggtcttgct aaaatggtta cagatgttat gggtaaaaat acagtaaaaa
    82441 gaatgccata tatgatacca tacataggaa gcttgttttt atttttcgca tgttcaaatt
    82501 taagcccatt acttggatta aggtcaccaa caaccgactt agatacaact gctgcatggg
    82561 caatgattac attttttatg atttattttg caggagttaa atttaagggg ttatcatact
    82621 ttaaagaatt ggctcaacca cttgccataa tgttaccaat caatattata ggtgagttag
    82681 ctagaccaat atcattaagt ttccgtccat ttgggaatat tttaggtgga acaataataa
    82741 tgggtctgtt ttatcaactt ttaggtttta tttctagttt aataccagga ttatcaatac
    82801 caattggaga aatattaata ccagtcccat tacacttgta ctttgactta tttgcaggat
    82861 tgttacaagc atatatattt ataatgttaa caatggtatt tatatcaagt gcaggagagt
    82921 tagattaaga aatcaactta tattaagata aaaataaaat aatttaaaaa attaaagttt
    82981 taaggaggaa ataattatgg aaacagcaat agtagcggca gcatcagcaa taggagcagg
    83041 gatagcagtt gcaacaggta taggtgcagg tataggtcaa ggtatagcag cagctaaagc
    83101 tgcagaagca gtaggaaacc agccagaagc taaaggtgat ataacatcaa ctttattact
    83161 aggagttgct atagcagagt cttcagcaat atatggtttg gttatatcaa taatacttct
    83221 attcgtaaat ccattcttta aatacttagg aatgtagatt tagtccttaa atattttgag
    83281 ataggtttat aggtaaatca tctcaaactt ataatcatga gatgaacttt atcatctcgt
    83341 gattataagt agttatgatt tatggaaagg aggataatta tggaaaaagc actagtaggt
    83401 ataacgtggg aatttgtgtt ccaaattgtt aatactttta taatattttt acttctgaga
    83461 aagcttttat tcaagccagt attaaatata attgaatcaa gagaaaatga tattaaaagt
    83521 gatttggtag aaggcgaaaa agcaaaaaat gaaggtttag ctctgaaaaa agaatatgag
    83581 tcaaaaatca attttgccaa agatgaaggt caagaaatca taaaacaagc aactataaga
    83641 gcagagcaga aatcagatga tatagtaaat actgcaaaga aagatgctct tgatataaaa
    83701 gaaaaagcaa acaaagatat agaacaagaa agacaaaagg ttataaatga aatcaaaaat
    83761 gatatatcta acatagcact acttgcagca tctaaagtta ttgaaaaaga tttagataaa
    83821 tctaaacatg aagaattgat agaaaacttt ataaaagagg taggggaagc taaatgataa
    83881 atgtaatagc aaatagatat gccgaagccc tatttcaatt aggagaagaa gaaaattcaa
    83941 ctgatgtctt attcaaagaa ctagaaaaag tagtagatat gatgactaaa gttagtaaag
    84001 atttctacaa agttttaaaa tcaccattag tttcaaaatc agaaaagaaa aacttggtag
    84061 aaattatttt tagtaaagaa gtaagttcta acataaaaaa ctttttaaaa gttttagtag
    84121 ataaagatag aatatcatat ttagaagata tcgaattagc ttataaagaa ttattaaata
    84181 agaaaaacaa tgtaattgat ggagtagcaa taagtgctat tccaatgagt gaaacagata
    84241 ttaaagaact tgaagtcaaa ctttcaaata aatacaacaa aaatgttact atagaaaatg
    84301 ttgttgacaa gactatacta gggggagttc tagttagaat tggaaatgaa caaatagatg
    84361 gcactgtgaa aactcgtctt gataagatga aagaaaagct atctgaagta atatcttaag
    84421 aatggcggtg aacccatgaa cttaaaacct gaagaaataa gttctataat taaacagcaa
    84481 ataaaaaatt atgagaataa agttgagttg acagatacag gtagtgtttt aacagttggg
    84541 gatggtatag cgagtgtata tggattagaa aaagctatgt ctggtgagtt gctagagttc
    84601 ccaggtgaaa tatatggtat ggcacttaac ttggaagaag aagtagttgg agctgttatc
    84661 cttggagatg acagtgaaat aaaagaaggc gatatagtta agagaactgg aagaatagtt
    84721 gaagttccag ttggagaagc tttaataggt agagttgtaa actcactagg tcagccaata
    84781 gatggaaaag gacctatagc gtacacaaaa actagaccag ttgaatctga ggcacctggt
    84841 ataatagata gaaggtctgt ttatgaacca ttacaaacag gaatcaaatc aatagattca
    84901 atgataccaa ttggtagagg tcaaagagag ttaataatag gtgacagaca aactggtaaa
    84961 acatctatag ttattgacac tatactaaat caaaaaggaa aagatgttat ctgtatatat
    85021 gtagcaattg gacaaaaacg ttctactata gctcaattag ttagctcttt agaaaaaggt
    85081 ggagctttgg attatacaat agtagtttca gcaacagcat ctgaatcagc accacttcaa
    85141 tatatagcac catatgctgg agcagctatg ggtgaagaat ttatgtacaa tgggaaacat
    85201 gtacttatag tgtatgatga tttaagtaaa caagctgttg catatagaga aatgtcattg
    85261 ttacttagaa gaccaccagg acgtgaggca tatcctggag atgtattcta tttacattca
    85321 agattacttg aaagagcagc taagttatct gatgaattgg gtggaggttc aatgactgcc
    85381 ttgcctataa tagaaactca agctggtgac gtttctgcat atataccaac aaatgttata
    85441 tctataacag atggtcaaat atacttacaa ccagagttat tctattcagg tgtaagacca
    85501 gcagttgacc ctggtatatc agtatcaagg gttggtggtt ctgcgcaaat taaagcaatg
    85561 aaaaaagttg caggaacatt aaaacttgca tattcacaat atagagaact tgcagcattc
    85621 tcacagtttg gttctgactt agatgaagat actaaaaaga gacttgctca aggtgaaaga
    85681 atcgttgaga tattaaaaca aggtgagcat caaccaataa aagttgaaaa tcaagttatg
    85741 ataatatatg ctgttataaa taatcattta gaggatattc cgatagataa tatagctaga
    85801 tttgaatcag aattgtatgc atttgtagat aataattatc cggaaatatc aagaaaaata
    85861 ttaggtggag aagacttcac tcatgattta acagatgcaa taaatgaatt taaagaaaag
    85921 tttgttgttg aagtataatc ctttttaaaa aaggaggtaa attaattggc aggagctgga
    85981 atcaaagcaa tcaaaacacg tatgggaact gttgaaagta caaagcaaat aactaaagca
    86041 atggaattag ttgcatcttc taagcttaga aaagcaaaag aaaaggctga agattcaaga
    86101 gaatatttta atactttata caatacagta aaagatattg cccaaacaac acgtggaatt
    86161 agaaatgaat ttttaaaaga aagagaagta aaaaataaat gctatgtagt agtagctgga
    86221 gacagaggac ttgcaggtgg atacaattct aatgtattaa aggaatctgt ctctcatatg
    86281 aaaggtaaaa aggaaacagt gattactgtt ggaaagaaag cacatgaatt tttttctaaa
    86341 agaaactaca atatagcaaa agatataaat agtgttgaag cttgtggata tgaagatgcg
    86401 acagaaattt cacaaactat tattgacctg tataaaaaag gtgaagtaga tgaagtttat
    86461 attgcttata caaaatttaa atctgcttta tcacaagaag ttgaaatttt gaaattgctt
    86521 ccattagtct ttgaaaaaga tacagacgaa caaggaatgg cagtaaaaga agaaaaacaa
    86581 aatagatcaa aagtacaata tataccatca gcagaagcag ttttaagtta tattgtacct
    86641 cagtttatat caggtacagt atatggtgga attttggaat cttttgcatc agagcaaggt
    86701 gctagaagaa ctgcaatgga atcagccaca gacaatgcga atgaaatgct atctaattta
    86761 gagttaagtt ataacagagc aagacagtca gctgtaactc aagaaataac agagatagtt
    86821 ggtggagttg aagctctaaa atagggaggt taggaaatgg caaatgtagg taaagtagta
    86881 cagattgtag gagctgtact agatgttaag tttgatagtg aacaaagcct acctaactta
    86941 ttaaatgcgt tagtaataaa gttaggagat aaagaaatag ttgcagaagt tgctcaacat
    87001 ataggtgatg atactgtaag atgtatagct atgagtgcaa cagacggact tgtaagaggt
    87061 atggaagttg tagatacagg agggccaata agtgttcctg ttggagatga aactttaggt
    87121 agaatattca atgttcttgg aaaaccagtt gatgggaaac cagctccaaa atctgcacct
    87181 aaattaccta tacatagacc agcaccggcg tatgatgaac tagaaactac agcagagata
    87241 ctagaaactg gtattaaggt agttgactta ctagctccat atctaaaagg tggaaaaata
    87301 ggtctatttg gaggagcagg agtaggaaag acagttctaa tacaagagct tataaacaat
    87361 attgctaagc aacatggtgg tatttctgta ttttcaggag taggagaaag aacaagagaa
    87421 ggtaacgacc tttatggcga aatgagtgag tctggagtta taaataaaac agctctagta
    87481 tttggtcaaa tgaatgaacc acctggagcg agaatgagag ttgctttaac tggacttaca
    87541 atggcagaac attttagaga tgagcaagga caagacgttt tacttttcgt tgataatata
    87601 ttccgtttca cacaagctgg ttcagaagtt tcagcacttc taggacgtat gccatcagct
    87661 gttggttatc agccaacatt agctactgaa atgggtgcac ttcaagagag aataacatca
    87721 actaagaaag gttcaataac atctgttcaa gcagtatatg taccagcaga tgacttaact
    87781 gacccagcac cagctacaac attctctcac ttagatgcaa aaacagtttt atcaagacaa
    87841 atatcatcac tgggtatata tccagcagtt gaccctcttg aatctacatc aaggattctt
    87901 gaccctagta tagtaggaaa agaacattat gaagtagcta gaggtgtaca atcaatactt
    87961 caaagatata aagaacttca agatataatt gctatacttg gtatggatga attatctgat
    88021 gaagataagt taatagttgc acgtgctaga aagatacaaa gattcttatc tcaatcattc
    88081 acagttgctg agcaatttac aggaaatcca ggtcaatatg ttcctgttaa agagactgta
    88141 agaggattta aagaaattct tgaagggaaa catgatgact taccagagtc agcattctta
    88201 tttgtaggaa caatagaaga cgctgttaga aaagcgaagg gaagtatgta gtcatggcca
    88261 gtgaattttc attgaaagtt gtaactccaa gtaatacttt ttatgatgga gatgttgaaa
    88321 tgatagtagt tagaactaca aaaggagaca aaggggtgtt gaaaggtcat ataccatttg
    88381 tttcaggtat acctgtagga actttaaaga taaaaaaaga gggaagtttt aaagaggcga
    88441 aaatagcaga aggattcatt aatgtaactc aagataaaac tgtcatattg acagaatctg
    88501 ctgagtggat ttaataaatt aatttagagt ataaaaagtc attttgacca aaaaaacttt
    88561 tgggtaaaat ggcttttttg tgttttggtg ttaataaaat aacgataaaa ttgtaagttt
    88621 ttagtgataa taaattcaca aaatgtttaa atttactgcc aaattatgtt gtaattaata
    88681 agataaagaa gatttagata gcttttatgt aagtgaaaaa gtcttcatat aagaaataca
    88741 tataaaattt ttataaaatt taaaaaattt gcttagttgt gtagtataat aaataatata
    88801 atagatttgt atataatatt cattacaaat aattttaaaa gttacagttt tattataatg
    88861 attattaaat taagataagt aacataaact aaataataat gtgtattatc gatagtaaat
    88921 ttataaattc attattaatt attgaaagaa gtattataaa ttttctggag gaacaatgaa
    88981 tatatttgat attggcgtag atataattga aattgataga ataagaaagg ctgtagacaa
    89041 aaataatagg tttttggaga agatatttac agatagagaa atagagtatt ttaattctaa
    89101 gaattttaaa gcagaaagta tagcaggtaa tttcgcagcc aaggaagcta taagtaaatc
    89161 tataggtaca ggaattagag tgtttaattt taaagatata gaagttctca gagatgaaat
    89221 gggaaaacca atagtaaaaa cttataataa tctagcaaaa atgtgcatag attataatgt
    89281 tttagaaata aaagtttcaa tatcacattc aaaagattat gctatagcaa atgcgataac
    89341 cataattaag gactaaggag tgattacatt atgatggata aaacagcaaa agaaataatg
    89401 acaacagatg taatagtagc aaaacaagat gatagtatag cagatgtagc taatatgcta
    89461 attgcagaaa aaataggagg tctgccagtt gtagattcag agaatagagt tgttggaata
    89521 atttctgaaa cagatattct taaaaaagaa aaatatatag aagcacctct ctatataaat
    89581 ctattacaag gattgatttt cttagatgac ttaaaaaaag ttgaaaaaga tataaaacaa
    89641 gtagctgctt ataaggttgg agaacttatg tctaaagata taataaaggt tcatgaagat
    89701 gataagtttg atgatgtagc taatgtaatg attaaaaaat caataaacag agttcctgta
    89761 gtagatgatg acaacaaact taaaggtata atttgtagat atgatataat aaaagcttta
    89821 tacaatgaat aaactgaagt aggaggtgtg atgtgagaaa aaagtggacc atagtatgta
    89881 ttatgttctt ggcactggtt ataattgtaa taggatgcca aaaacgacag tccacaaaag
    89941 aagaagtata caaagatttt caaaagcaga tttctgatat gaattattac agttgtaaag
    90001 ctgaagtaga agtagttgga aataaaagtc cacacaatta tgttttaatt catacttata
    90061 aaaaaaccga taactataaa ttagaagtaa tttccccaaa acatttaaaa gggaaaagta
    90121 tagaatatca aggtgataaa atattagtta aaaatccaaa gattagtgat gtagtggagt
    90181 tacctaatac aggaaaaaat aatcaatatc tttttgtagg agactttata aaaaactatc
    90241 ttcaaaatga ggagatgaaa gtaaagttat ccaaaggaca tttggtttta gagactttta
    90301 ttcctgggga taataaatat tttaacaagc aagtattata tgtaaatgct gacacaaaaa
    90361 atcctgaaaa aatggaagtg ttagataaag agggagtgcc aagatttaca gtaaaataca
    90421 aagattttga atacagaaac taagggggac taaagacatg caaaaaataa cagtgcctac
    90481 atgggcagag ataaatctag ataacttaag atttaactta aataatatta aaaatttatt
    90541 agaagaagat attaagattt gtggagtaat aaaagctgat gcatatggac atggtgcagt
    90601 agaagttgca aaattgctag aaaaagaaaa agtagattac ttagcagtag caagaactgc
    90661 tgaaggaatt gaacttagac aaaatggcat aacacttcct attttgaact tgggatatac
    90721 tccagacgaa gcttttgaag attctataaa aaataaaata actatgacag tttattcttt
    90781 agaaacagca caaaagataa atgaaattgc aaaatcttta ggagaaaaag cctgtgttca
    90841 tgttaaaata gactcaggga tgactagaat aggtttccaa cctaatgagg agtcagtaca
    90901 ggaaataata gaattaaata aattagaata tatagattta gaaggtatgt ttactcattt
    90961 tgctacagct gatgaagtaa gtaaagagta cacttataaa caagctaata attataaatt
    91021 tatgtctgat aaattagatg aggctggtgt aaaaatagct ataaaacatg tatcaaacag
    91081 tgcagctatt atggattgcc ctgatttaag attaaatatg gtaagagcag gaataatatt
    91141 atatggtcat tatccatctg atgatgtatt taaagataga ttagaattaa gaccagccat
    91201 gaaattaaaa tcaaaaatcg gacatataaa acaagttgaa ccaggtgtag gaataagtta
    91261 tggactaaaa tacacaacta caggtaaaga aacaatagct acagttccaa taggatacgc
    91321 agatggattt actagaatcc aaaaaaatcc aaaggttctt attaagggag aagtgtttga
    91381 tgtagttggt agaatatgta tggatcaaat aatggttaga attgacaaag atatagacat
    91441 aaaagttgga gatgaggtta tactatttgg agaaggcgaa gttacagctg agcgtatagc
    91501 taaagactta ggaactataa actatgaagt gttatgtatg atatcaagaa gagttgaccg
    91561 tgtttatatg gaaaataatg agcttgtaca aataaacagt tatttgctaa aataaaacca
    91621 tgatattata atctgggagg cgattttgtg tacgataaga gtaaagaaaa aatagaagtt
    91681 actttacctg actctctgat ttctgaagtt gatagtatag ttcagatgga gaacagtaac
    91741 agagaagatt ttgcaaaagc tgcttttcag ttctatataa ctcagaaaaa gaaaattcat
    91801 attaaagaat ctatgaaaac gggttataga gaaatgggtc aaattaatct gtcactagct
    91861 gaattaggta tgacagttga agaagtatct tctgatgata aaaatgaagg gaagattgcc
    91921 caaggtgagt actaattgtg aaataaagcg tggtgattta tattacgctg atttaagtcc
    91981 agttgttggt tcagagcagg gaggcgttag acctgttctg ataatacaaa atgatgtagg
    92041 taataaatat agtcctacag ttatagtatc agctataaca tctcagatta ataaagccaa
    92101 gttacctact catgtagaga taagttccaa tgaatatggg cttaataaag actcagtaat
    92161 tcttttagag caaattagaa ctatcgataa aaagagactg agggaaaaaa ttggttgtct
    92221 agatgaaaat atgatgataa aagtagatga tggacttcaa ataagtttag gattgtttac
    92281 tttttaaaga gggatttgtc ttttattaga caatcccttt tttcttttaa atatctctta
    92341 aggtttaatt ttaatgtact atgttaataa aattaaaaat aacgcaaaaa aatagcataa
    92401 aagtgatata ataatatgga attgatataa aattacgaac tcaagggagg taattgcatg
    92461 agagaccata aaggattaaa tgagattgcg cgtatcataa gaagagatat agtttcaatg
    92521 atacatagag ccaaatctgg acatccagga gggtcattat cagtagttga aatacttact
    92581 gcgttatact ttgatgaaat gaatgtggat tcttcaaatc ctaaaatgga ggatagagat
    92641 agatttgtat tatcaaaagg acatgctgca ccagcattat atgctacatt ggctgaaaaa
    92701 ggttattttg ataaggaaga actgaatgga ttaaggaaga taggaaggat gttacaaggt
    92761 catccagaca tgaaaggcac gccaggtgtg gaaatatcta ctggttcttt aggtcaaggt
    92821 ttttcagttg catgtggtat ggctatggca tcaaagttgg ataatgctcc gtggaatgtg
    92881 tatactttgc ttggtgatgg tgaggttcaa gaaggaattg tttgggaagc tgctatgagt
    92941 gctgcacatt ataaacttga taatttaatt gcttttttag ataataatgg acttcaaata
    93001 gatggagata tagaaagcgt aatgagttta ggttcaatag ttgataaatt taaagctttt
    93061 ggttggaatg taattgagat agatggacat gattttgacc aaatatttgc ggctcttgat
    93121 atagctaaat caacagttaa aaaacctaca atgatagttg caaaaactat aaaaggcaaa
    93181 ggaatatctt ttatggaaaa tcaagcagga tggcatggga cagctccaag tgatgaggag
    93241 ctagaaaaag ccttgcttga attaggaggt gctgacaatg agtaaaatgg cgacgagaga
    93301 agcatatggt aaagcattag taaaattggg tcaaataaat gacaatgttg tagtattaga
    93361 tgcagattta tcaaagtcta caaaaacaca tgatttttac aagtcatttc cagatagatt
    93421 ttttaatatg ggtatagctg aacaaaattt aataggcgct gcttgtggat tatcaactgc
    93481 tggtaaaata ccttttgcaa gcacttttgc tatgtttgct acgggaaggg cttttgagat
    93541 tataagaaat tcagtttgtt atccaaagct aaatgtaaaa atatgtgcta ctcatgcagg
    93601 tcttactgtt ggagaggatg gagcatctca tgaaagtgtg gaagatattg ctataatgag
    93661 agctatacca aatatgacag ttcttgttcc agctgatgga gttgaaacag aaaaaattat
    93721 ttttgaaata gctaagtata atggacctgt ttatgttaga ttagggagaa gttcagtacc
    93781 agttttattt gatgaagatt ataaatttga aataggaaaa ggtacagtct taagagaagg
    93841 aaaggatgta tcaattatag cttgtggaat tatggtaaat gaagctctat tagcacaaga
    93901 aaagttgcaa gaagaaggta taagtgctag agttataaac atgtcctcga taaaacctat
    93961 agacaaagat ttaatattag aatcagcaaa agaaacgaat gctattgtaa ctgtagaaga
    94021 acatagcata attgggggat taggttctgc tgtaagtgag gtagtaggag agtcttgtcc
    94081 aaccattgta aagaaagttg gaattaaaga tacatttgga gaatctggta ctccaaatga
    94141 attgttaaaa aaatatgaat taacttgtga tgacataata aagactgtta aagaagctat
    94201 tattgcaaaa agaatgtaag agatataaat tattcatgtt tgttaataat ataatatatt
    94261 taaaagagag gtataaagtg ttatattaat ttatatctct ctttttttta tatgatatca
    94321 atatatcaaa aatgtaataa aatattcaaa tttttacaaa aatagagaaa atattcaaat
    94381 atatattgac tattcagaaa aatatgacta atatataatt tatagagaaa ctaattttat
    94441 atataatgac tacttagata aaagaatcta aaaaaattat gagaggagga gtagggtata
    94501 aaatcagatt ataagtttta agttattata ctaaaaagct ataaaacagt tatcatgtat
    94561 tttatgttaa aggaaattgt aggtagttta tcgaattgtt taatcagaag aaaaaatttc
    94621 acatggggag gtaactgaga tatgtcagaa aaagtaaatg ttaaaacagt catcagtttc
    94681 gcaggtgcgt atgtagcaac agtaatcggt tctggatttg caacaggaca ggaaataatg
    94741 caattcttct ctttttatgg ttttgcaggt attattggtg ggataatttc aatggtatta
    94801 ttctcatgga tgggtgcttc agttatgtca aaaggtaaag agcttcaact taaagaacct
    94861 atcaaaattt atcgtgttta ttgtggtaag tatttaggaa tattctttga atggtttgga
    94921 cctttattct tatttggtgt tttcgtagta atgatttcag gagcaggagc aactttaact
    94981 gaatactatg gattaaatcc atttgtaggt agagtaggta tggcagtagt agcattatta
    95041 actgtttcac taggtcttga taaattatca aagatactag gtggaatagg gcctataata
    95101 ataatcttta cacttttagt tggtggtata agtttagcta aaaacatagg taatatagga
    95161 gaagctgcac aagttcttag cactgtaaag atagctaaac cagttccaaa tgcctatcta
    95221 tctggtgtaa tatatactac ttataacaca attgttgtta tggcattctt aactggatta
    95281 ggagcaagtg ctgctaacaa aaaagaagct atttgtggtg gtatattagg tggagtagca
    95341 cttatggctg cagctataat gatgcactta gctattcttt ctgatatagg aaacttatat
    95401 tcaaaagcaa taccatcatt attcttagca gataagattt caccagtaat tggagtttta
    95461 ttctcaataa ttcttatatt aggtatatat acaacagctg tacctttatt atggtcagta
    95521 actaatagat ttgtagatga caaacatcct aagtttaaat taataacttt aatagcagct
    95581 atattaggtc ttataggtgg cttcctacca tttgataagc tggttggtat tttatatcca
    95641 tatactggat atatgggcgt cataatactg gtttgtgttc tttatagaca gattacaaaa
    95701 acatctggat atcaagaagg aatcagtgac aaataagtat tttttaatat tgtaaactca
    95761 agagctatat gaaaacaatg aaaatgtttt catatagctc tttttatttt ttagctcgtt
    95821 tattatgaaa tagaaaaaac atgtttttta gttacattac atatatttat aattatgtat
    95881 aaatatatac taatcaataa tttagagtct aagataagtt tataaatcat atatttagaa
    95941 tatatgggag gagtgataag attgaatgta aagtttaata ttaaaggtat aatttatgga
    96001 gttatagctc ttgtattcgt tataggggga tttatattca taccaagtat gtttgtagag
    96061 aaatctaaac ccatagatta tacagtatta caaagaaatt caataccaga aaagatatta
    96121 gatgtgatgg ataagtatac aaatgaagaa agagcattag cggctaaaat cgataacaaa
    96181 atatacatta tagtgactag aggaaataat aaatatggta tagaaatgga caaaatagaa
    96241 agtgtggttg aggaaggaaa ggatgttttg agagtcaaga taaagtataa agataaggaa
    96301 gattcttttc catacatagt tgttgaaaca aatatgagtg aacttccaga tagaatagaa
    96361 ttaaatgcga cgaaataaga aggtatttta taattaaata actgcatatt gtacaataaa
    96421 gtcatggttt ttattaatgt aagtaaattg tgtatggttc ttattgttgt aaaagccatg
    96481 acttaaaatg cttataaagt aagatattaa cagtagtatt tattactaca ttaaatgggg
    96541 tctggaaata cttaatattc taactatttg gagtgataat tctagtttca gtaactatgt
    96601 agtctaattg tgcatcatga gtttcttttg gaatagaatc gaatatctgg aagtcaaatg
    96661 ctatgccgat agttatagca tcttttctta agttttctaa aaatctatca taaaatcctc
    96721 caccatatcc taatctatag caatgtatat catatgctac agctggtaca attacaacat
    96781 ctaaggcctt aatatcaatt gtaggtgatt tttcgttagg ttctcttatt ccatatgctc
    96841 caattctcaa tccctctttt aaatctgtta tttgagtagg gattaatgta tgattttctt
    96901 ttattgtgat gggacttgat actattttct ttagagacaa aagttttttg attaaattat
    96961 ctgtttgaac ttcattgtta aaatttagat aaagcattat attagttgca tttcttatat
    97021 cttccatttg aagtaatttt tcagttatta aatttgaatt actaagtaca aagtttgcat
    97081 tcttattttt tcttgattca ataacttttt ttctaaattc ttttttcata aaataattcc
    97141 tcccttaata tgttataatg tgtacagcaa ttgtatttaa tataaatttt agtataattt
    97201 attgtaataa agcaatatat ttgggtaaat gtaataaaaa taacagctag tcatataata
    97261 ataatagttt atgtaaaagg agatgtatct tgtgatttca aagaagaaag ctattttttt
    97321 aggggtgatt ttagtaatta taacagctat ggttacatca gcatttcagt tgacattggg
    97381 gaataaagtt gtaatatcga aagaactcta tgaagactat aaaaaatatg ataagttgtt
    97441 aggactagaa tctattataa aacaagattt ttataaaaaa gtatcagata ctgatttggt
    97501 aaatggagca gcaaaaggtc tatttctagg aacaaatgat aagtattctg gttattatac
    97561 taaagatgaa atggagaatc taattaatga tagtgaaggt tcttatgttg gtgtaggtat
    97621 gtatataggg gcatcaaaag atggtggctt agtagtagtt cccatgaaag attctccagc
    97681 agagaaagcc ggtgtaaaat caggggataa attagtaaaa gtcaatggaa aatctgtctc
    97741 atataaaaat tcagatgaag ctgttcgtat gatgaagggt aaaaaaggta aaacagttga
    97801 actgactata ttaagagaag ataaacaatt gaactttaaa gtaaaaacag accagataat
    97861 tgagaagagt atagaaagta aagtaattga taatgattta ggatatatag aaattaccca
    97921 atttatatca agtacatata ctgactttga taaagcttta aaagaattaa aagcaaaaaa
    97981 tatcaaggga ttagtaatag accttagaaa taatcctggt ggaatgctag atatatgtaa
    98041 agaagttgct gatgagttaa ttggtgaagg aactatagta tatacaaaag ataataaggg
    98101 aaatactgag tacttgaaat ctgataaaga aaaattggga cttccaatag tagtgttaac
    98161 taatggagaa agtgcttctg ctgctgaaat attaacagct gcaatcgttg ataataaaga
    98221 aggtatttct gtagggacta caacttttgg taaagggttg gtacaatcag ttgtaagatt
    98281 gaaggatgga acaggataca agctaactac tgctcaatat tttacaccta atggggatta
    98341 tatcaatgaa aaaggtataa agcctactat tgaagagaaa gatgaaaata aacagttaga
    98401 tgtagcaact aagtggctta gagaacagat tgataaatag gatttaattt ttataaactt
    98461 atactataaa ataaagaaca ttataaaaga gatgtctcaa aatgaacttt ttagttcatg
    98521 agggagtctg gaaatattta tgtgtattct ttaaataaaa atttattttt taagtgataa
    98581 ttaaattttt acagacaggg ctatggtttt aaaaatcata gccttatttt gatatttttt
    98641 aaaaacatat tgcaacccag ttgttccaaa tattatatca tttactttta aaatatcgca
    98701 aactgcatat tatatattca ttataatata atttatctaa ctagaacctc agtttactaa
    98761 ttaaattaga tgcatagaat aactttttta aacattgaaa ataaattaaa ctctaaacac
    98821 atttaaatat aaatttatat atcattttga cttggttaaa caatttcata cttatcgata
    98881 taaaaatagg ttataatata aatgttcatt atcttataac ctattttata taatttattc
    98941 ctaaaacatg tgaatgtttt ttatgatagt ttgaagaaat tataaaatca ctttataatt
    99001 gaatattata tataaattaa tttaaatata gaaaggaaaa tatatgtaca acaatatcaa
    99061 aaatatttta aaagaaaatc atataacgtt tgaagaatat gaccatgaac ctattttatc
    99121 atttgaaaaa gctaaagaaa taagagaaaa attacaatat actggagtag aaagtaaaag
    99181 tttatttata aaagataggt ctggaaatta ctatatattt gtaaccacag aaggaaaaaa
    99241 attagatcca aaaactataa aaaatgtttt aggtggaaaa gtatctatat gttcaaaaga
    99301 agaattaaca caaaaaacac aatgtttgcc tggatgtgta tctccttttg gatatgagaa
    99361 aaatatctct cttgtaatag atcaagatat atttaaccat gataaaataa tcttttcacc
    99421 agggatacca gaaaagacat ttattattac agctagtgga tttaaaaaag taataaataa
    99481 tataaacaat acaacttata aatatatata tattaatgaa ttttaatcat aattgtaaat
    99541 tagagaaaat aacattagaa atcatgctta actatagtgt aaataaatta tattcaacac
    99601 aaaagattga tactattatt acaatagtta ctgttaagct caaattcaat gtattatgag
    99661 ttattatttt taaattttaa ttacaaaagc agggtagttt taactactct gctttatatt
    99721 taacataaaa tatataaaat caacaaaatg attttagtct atctattaga tattcttcgc
    99781 tctcaatgct tagaactttt agatatatac aataagtgct ttcatttgta aaaatttcta
    99841 tagtattatc ttgtttattt acacctacaa tatctttaaa attgatatga tttttagtat
    99901 ttttacattt gaaaatatat ctgttatcaa ttaaaatctc atcattagta attgaaatat
    99961 agggtttatc tcatgcttta ttaaaaattt atatgtttgt tcaagataaa aacttacaaa
   100021 taataataga aaagttactt taaatgtaca tattatcgaa aatattatca caaaaatact
   100081 tataatatat atttgtttaa aataacctct atcgcaatag tttattaacg atatggaaat
   100141 aaaagtgtta aatcagattt tggagaaata actttaaata tttccagact ctttttagac
   100201 agcctccata accttatatt gaacctattt attaactttg tacttacata taccacttat
   100261 ataagtttcg tatatattaa agtccttatc tgttactata aaatctgcct ccataccttg
   100321 atgtatggag ccacaaacat tatcaatatt acaacttaaa gcagggatat atgtagccat
   100381 ttttattgca tcttctaaag tggctatgtt ccaatcaact atatttctca cagccttgtc
   100441 catagttaat acacttccag ctaaactccc actatttaat cttgcaacac tattttttac
   100501 atttacatta aacttaccaa gagtatattt tccatcctcc atagcaccag ctctcataca
   100561 atctgtaatt aaagctatgt tattactaga cttagcatcc atcattattt taacagctat
   100621 tgggcttaca tgatgaccat cacaaattaa ttcagcataa gtatctttta gtaccatagc
   100681 agccccaacc attccaggtt ctctatgatt taaaggactc atcccattat atgtatgtac
   100741 aaacacttta gcacctgaat taacgacttg tttaacttgc tcaaatgtag ctgaactatg
   100801 accaagagaa actgatatat tatttttagt tgcatatctt gttaattcta ttgaaccttt
   100861 acgttctgga gcaattgcta tttttcttat taaatttttc gatttttttt gccataattt
   100921 taatatattg atgtcagggt ctttaaaata tttttcattt tgagcgccct tatatttttc
   100981 tgtaaaaaat ggaccttcta aataaatccc ttgtattttt gcaccctcaa ctcgattgaa
   101041 gctattgctt atattttcta cagaatcact tagtgtatct acagaatcag ttaaagttgt
   101101 aggtaggaaa gatgttactc catattttaa taagcctttt gaaattgtgt ttattgcttc
   101161 aaatgtattg tccatgacat ctgcacctaa aagaccgtga atgtgagtat caactaagcc
   101221 aggagcaata tatttacctg aaaaatctat aacattatca gtaacattag gtctttcttg
   101281 ttgaaattca ccgaatttgc cattaattat actaaggtaa cctttttctt tgattccatg
   101341 tttcataaaa aatttatctg cataaataaa acttttcaaa ataacctcct tataattata
   101401 ttatcattgt ggcattgacg cttataatat cattctgttt tcttgaaagt tatttaactt
   101461 actatcttac atgtcttact ctatatacaa atttatcact tctagcgaca ctcaaagtaa
   101521 attcaataac tatattgtca tcattatatg tggtacgaag taatttcaga caaggtgaat
   101581 ctcctggtac ttccaataac ttagcttcct tatccgagat tattccagct gaaaattctt
   101641 catcagcaac tttaataatc tcgccataat cttctctaaa tatttcatat aaaggttttt
   101701 gttcgatttt ttcttttgtc aatcctataa attctttcat aggaagatat gttctttcaa
   101761 gcatcatagg aaggtcatca gcaagtctta aacgcattat ttttatcatt ttttcaccag
   101821 gagttatacc taatttttca gagaaatact ttgtactttc tactacctca aatgataata
   101881 cctttgtacc tggctttttt ccttgtgttc tcatataatc tgtaaaacta taactttcca
   101941 ttaagttttg agaattttga cttagtgcag caacaaaagt tccttttcca tgtcttttgt
   102001 atatatatcc catatgctct aactcagcca tagctagtct aactgtggtt ctacttactc
   102061 catattcatc acaaattttt ctttcggaaa gcattctatc gtttggttcc atctcatttt
   102121 caatcttagt tcttaatatc tcaacaagct ggtcatatat tggtctttta tcatttcttt
   102181 ttatcataaa aatatagtcc tttctaagtg gttattacca caatttgatt atagaatata
   102241 gaattattta tgtcaatgaa taaagtttaa tcaaaattca ataagtaagg ctattaattt
   102301 attataaaat tatggtacat tgacaaaaca agtaaaataa tttatattca aattggtaat
   102361 aaccactttg gatatattat atgaggtatt ttttatttta cagattaatt tattgtttag
   102421 aaaagtaaaa attaataatg aggagaatct agtatgatta cagtaataac attcaaccca
   102481 tctatagata gaatgtatag agtgaataat ataaacatag gtgaggtgca aagggtagta
   102541 agtaagaatg caactgctgg aggtaaagga ttaaacgtat ctaaggtatg taaaatttta
   102601 aaagaaaatc ctttagctat gggatttcta ggcggattta atggagaatt tataaaaaat
   102661 gaacttagaa aactagatat acaaaataaa tttacaaaaa tagagcagga aactagaaat
   102721 tgcttaaaca taattgagga caataaagtt agtacagaat ttttagaaaa aggaccaata
   102781 gtagaaaaaa gcgatttaga gagatttgaa aatgatatag aggaagtact aaaaaatact
   102841 aaaatactgg tagcttctgg tagttattgt gagaatatgc cgatagacta ttatgaaaag
   102901 ataggaaata tatgtagagc aaataatata aagtttatat tagatacatc aggagaagca
   102961 ttaaaaatag ctttaaaatc aaaaccatat ttaataaagc caaatataga tgaaataaga
   103021 catttattag gaataaatat agaatcaaga gaggaaatta tcttgtcagg aaaaaaactt
   103081 attgaaatgg gtgcagagaa tgtatgtata tctttaggaa aagatggtat ggtgtattta
   103141 aacgaagata acgtatatta tgtaaaagta ccagttgtaa agtgtataaa tactgtaggt
   103201 agtggagata gcacagtggc tggatttagt gtaggtattt taaggggtta taaaatagaa
   103261 aatttactta aatttgcaaa tgcttgtggt ataagtaatg ctttaaatat ggaaactgga
   103321 tttgtagatt tgggagaaat tgataaatat caagatttag ttgaagtaaa aaagttaagt
   103381 taaatacatt taatgggaat ataataaatt taatgttgtt gtaatagatt ttaattttaa
   103441 tataaaagat atagtcttat ataaaaatag gttagaaaaa ttcattttta acctattttt
   103501 ataataaata tctattttac tgaatttata attttctcta gtaatctttg agccttgata
   103561 cactcttctc cactgatagc tggttttata ttattttcta tacaatcaat aaaatgattt
   103621 gatatatcct caaaaccctt ttgttttaat atatttaccc atgcaccact ttgctttaaa
   103681 gttaggtttc caccttcttc tatttccagt acattaagat tcttaactct ttgaatctta
   103741 ccctttgaaa gaatttcaac ttgttctaac tttgttccag aatctctatg catagaggtg
   103801 tttatagaga aatttttacc tttaagttta tgacttacaa atataagatt cttattgtct
   103861 gttaaaaata aatcctctcc actaatttca acatcttcat ttgctaacca taaagcagtg
   103921 tcaataacat gtatatagtc atctataaga gtactgtcga atttaacatt tcttaaggaa
   103981 tttaatccat gtttacatat attgatagat acaatttctg tagcattatt tttaatttct
   104041 ttgtacatag gacagaatct acggttgaat ccaaccatta aatttaagtt ttttttcgta
   104101 cttaattcga taagttcttc tccttgactt actgttgaag caagaggttt gtctacatat
   104161 acatgaacac ctaaatttaa taatattttt ataatttcgt agtgtgtttc tgtagagcta
   104221 tgtagaaaaa tacaatcaca ttttttagct agggattcaa tagaatcgaa ttgcatgatt
   104281 ctataatcac tacagacttt ttccctttta actttatttg gagtaaaagc tcctacaaac
   104341 tcaaatctct cagacttagt taatataggt aaatatgcct tttgagctat ggagccaaga
   104401 cctatcattc ccatttttat atttttcata atactttctc ctaaggtttt tatatttaca
   104461 gaattgatta taattcagac acataaattt gtaaactttg tatcaaaaaa taaatttaaa
   104521 gcttatataa gtaaattagc gaaataagaa atattgataa agtaattttg taggatatct
   104581 ataaaattga ttgatataaa tattttatgt attgacaaaa aattcaaatt aatttatatt
   104641 tggattagtg gtaattacca ctttggtctg aacaagtttt ta
//
LOCUS       DBGJFU010000002        80179 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.002, whole genome shotgun sequence.
ACCESSION   DBGJFU010000002 DBGJFU010000000
VERSION     DBGJFU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 80179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.002"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(121..894)
                     /locus_tag="ACOIAS_000106"
     CDS             complement(121..894)
                     /locus_tag="ACOIAS_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HGZ2699841.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1301)
                     /locus_tag="ACOIAS_000107"
     CDS             complement(894..1301)
                     /locus_tag="ACOIAS_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2699842.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="ACOIAS_000108"
     CDS             complement(1305..1622)
                     /locus_tag="ACOIAS_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HGZ2699843.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="ACOIAS_000109"
     CDS             complement(1634..1894)
                     /locus_tag="ACOIAS_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HGZ2699844.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="ACOIAS_000110"
     CDS             complement(1894..2190)
                     /locus_tag="ACOIAS_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGZ2699845.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="ACOIAS_000111"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="ACOIAS_000111"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HGZ2699846.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="ACOIAS_000112"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="ACOIAS_000112"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGZ2699847.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKRYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="ACOIAS_000113"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="ACOIAS_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGZ2699848.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="ACOIAS_000114"
     CDS             complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="ACOIAS_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HGZ2699849.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8082..9188)
                     /gene="dnaN"
                     /locus_tag="ACOIAS_000115"
     CDS             complement(8082..9188)
                     /gene="dnaN"
                     /locus_tag="ACOIAS_000115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGZ2699850.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9430..10749)
                     /gene="dnaA"
                     /locus_tag="ACOIAS_000116"
     CDS             complement(9430..10749)
                     /gene="dnaA"
                     /locus_tag="ACOIAS_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGZ2699851.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11408..11545
                     /gene="rpmH"
                     /locus_tag="ACOIAS_000117"
     CDS             11408..11545
                     /gene="rpmH"
                     /locus_tag="ACOIAS_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGZ2699852.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11623..11967
                     /gene="rnpA"
                     /locus_tag="ACOIAS_000118"
     CDS             11623..11967
                     /gene="rnpA"
                     /locus_tag="ACOIAS_000118"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGZ2699853.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11999..12250
                     /gene="yidD"
                     /locus_tag="ACOIAS_000119"
     CDS             11999..12250
                     /gene="yidD"
                     /locus_tag="ACOIAS_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGZ2699854.1"
                     /translation="MNKIIYILKEISKHLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12282..12989
                     /locus_tag="ACOIAS_000120"
     CDS             12282..12989
                     /locus_tag="ACOIAS_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HGZ2699855.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12958..13587
                     /gene="jag"
                     /locus_tag="ACOIAS_000121"
     CDS             12958..13587
                     /gene="jag"
                     /locus_tag="ACOIAS_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HGZ2699856.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13699..15078
                     /gene="mnmE"
                     /locus_tag="ACOIAS_000122"
     CDS             13699..15078
                     /gene="mnmE"
                     /locus_tag="ACOIAS_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGZ2699857.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENVIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15173..17068
                     /gene="mnmG"
                     /locus_tag="ACOIAS_000123"
     CDS             15173..17068
                     /gene="mnmG"
                     /locus_tag="ACOIAS_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGZ2699858.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17061..17780
                     /gene="rsmG"
                     /locus_tag="ACOIAS_000124"
     CDS             17061..17780
                     /gene="rsmG"
                     /locus_tag="ACOIAS_000124"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGZ2699859.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17943..18728
                     /gene="noc"
                     /locus_tag="ACOIAS_000125"
     CDS             17943..18728
                     /gene="noc"
                     /locus_tag="ACOIAS_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HGZ2699860.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18863..19636
                     /locus_tag="ACOIAS_000126"
     CDS             18863..19636
                     /locus_tag="ACOIAS_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2699861.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19638..20501
                     /locus_tag="ACOIAS_000127"
     CDS             19638..20501
                     /locus_tag="ACOIAS_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGZ2699862.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20534..21676
                     /locus_tag="ACOIAS_000128"
     CDS             20534..21676
                     /locus_tag="ACOIAS_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HGZ2699863.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="ACOIAS_000129"
     CDS             complement(21810..22385)
                     /locus_tag="ACOIAS_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HGZ2699864.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /gene="rstA"
                     /locus_tag="ACOIAS_000130"
     CDS             22645..23937
                     /gene="rstA"
                     /locus_tag="ACOIAS_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin production/motility transcriptional
                     repressor RstA"
                     /protein_id="HGZ2699865.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="ACOIAS_000131"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="ACOIAS_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGZ2699866.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="ACOIAS_000132"
     CDS             24717..24980
                     /locus_tag="ACOIAS_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGZ2699867.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="ACOIAS_000133"
     CDS             24995..25180
                     /locus_tag="ACOIAS_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HGZ2699868.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="ACOIAS_000134"
     CDS             25283..26479
                     /locus_tag="ACOIAS_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2699869.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /gene="rpsF"
                     /locus_tag="ACOIAS_000135"
     CDS             26693..26971
                     /gene="rpsF"
                     /locus_tag="ACOIAS_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGZ2699870.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /locus_tag="ACOIAS_000136"
     CDS             27008..27442
                     /locus_tag="ACOIAS_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGZ2699871.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /gene="rpsR"
                     /locus_tag="ACOIAS_000137"
     CDS             27461..27688
                     /gene="rpsR"
                     /locus_tag="ACOIAS_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGZ2699872.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="ACOIAS_000138"
     CDS             27831..28151
                     /locus_tag="ACOIAS_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HGZ2699873.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="ACOIAS_000139"
     CDS             28159..29115
                     /locus_tag="ACOIAS_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HGZ2699874.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="ACOIAS_000140"
     CDS             29126..31120
                     /locus_tag="ACOIAS_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HGZ2699875.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /gene="rplI"
                     /locus_tag="ACOIAS_000141"
     CDS             31121..31570
                     /gene="rplI"
                     /locus_tag="ACOIAS_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGZ2699876.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="ACOIAS_000142"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="ACOIAS_000142"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGZ2699877.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="ACOIAS_000143"
     CDS             33122..33430
                     /locus_tag="ACOIAS_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2699878.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="ACOIAS_000144"
     CDS             33512..34801
                     /locus_tag="ACOIAS_000144"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGZ2699879.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="ACOIAS_000145"
     tRNA            35127..35202
                     /locus_tag="ACOIAS_000145"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="ACOIAS_000146"
     tRNA            35209..35283
                     /locus_tag="ACOIAS_000146"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="ACOIAS_000147"
     tRNA            35288..35363
                     /locus_tag="ACOIAS_000147"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="ACOIAS_000148"
     tRNA            35397..35471
                     /locus_tag="ACOIAS_000148"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="ACOIAS_000149"
     CDS             complement(35854..36846)
                     /locus_tag="ACOIAS_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2699880.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="ACOIAS_000150"
     CDS             complement(36855..37913)
                     /locus_tag="ACOIAS_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGZ2699881.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="ACOIAS_000151"
     CDS             38038..39501
                     /locus_tag="ACOIAS_000151"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HGZ2699882.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="ACOIAS_000152"
     CDS             39603..40400
                     /locus_tag="ACOIAS_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2699883.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="ACOIAS_000153"
     CDS             40413..42515
                     /locus_tag="ACOIAS_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2699884.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="ACOIAS_000154"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="ACOIAS_000154"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGZ2699885.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43211..43522
                     /locus_tag="ACOIAS_000155"
     CDS             43211..43522
                     /locus_tag="ACOIAS_000155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2699886.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43570..43887
                     /locus_tag="ACOIAS_000156"
     CDS             43570..43887
                     /locus_tag="ACOIAS_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2699887.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44100..44801
                     /locus_tag="ACOIAS_000157"
     CDS             44100..44801
                     /locus_tag="ACOIAS_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2699888.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45025..46326
                     /locus_tag="ACOIAS_000158"
     CDS             45025..46326
                     /locus_tag="ACOIAS_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2699889.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46463..47557
                     /locus_tag="ACOIAS_000159"
     CDS             46463..47557
                     /locus_tag="ACOIAS_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HGZ2699890.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            47559..48692
                     /locus_tag="ACOIAS_000160"
     CDS             47559..48692
                     /locus_tag="ACOIAS_000160"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HGZ2699891.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48710..49819
                     /locus_tag="ACOIAS_000161"
     CDS             48710..49819
                     /locus_tag="ACOIAS_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HGZ2699892.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49884..50624
                     /locus_tag="ACOIAS_000162"
     CDS             49884..50624
                     /locus_tag="ACOIAS_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HGZ2699893.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGYVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50629..51621
                     /locus_tag="ACOIAS_000163"
     CDS             50629..51621
                     /locus_tag="ACOIAS_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGZ2699894.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVLICGIAMQLTDSIKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51809..52690)
                     /locus_tag="ACOIAS_000164"
     CDS             complement(51809..52690)
                     /locus_tag="ACOIAS_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2699895.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52963..54351
                     /locus_tag="ACOIAS_000165"
     CDS             52963..54351
                     /locus_tag="ACOIAS_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HGZ2699896.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54379..55332
                     /locus_tag="ACOIAS_000166"
     CDS             54379..55332
                     /locus_tag="ACOIAS_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2699897.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            55392..55616
                     /locus_tag="ACOIAS_000167"
     CDS             55392..55616
                     /locus_tag="ACOIAS_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2699898.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55609..56121
                     /locus_tag="ACOIAS_000168"
     CDS             55609..56121
                     /locus_tag="ACOIAS_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGZ2699899.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQEDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56153..57007
                     /locus_tag="ACOIAS_000169"
     CDS             56153..57007
                     /locus_tag="ACOIAS_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HGZ2699900.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57541..58335
                     /locus_tag="ACOIAS_000170"
     CDS             57541..58335
                     /locus_tag="ACOIAS_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2699901.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58439..59245
                     /locus_tag="ACOIAS_000171"
     CDS             58439..59245
                     /locus_tag="ACOIAS_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGZ2699902.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SITVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINEDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59307..59828
                     /locus_tag="ACOIAS_000172"
     CDS             59307..59828
                     /locus_tag="ACOIAS_000172"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGZ2699903.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60243..62330
                     /locus_tag="ACOIAS_000173"
     CDS             60243..62330
                     /locus_tag="ACOIAS_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2699904.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62345..62632
                     /locus_tag="ACOIAS_000174"
     CDS             62345..62632
                     /locus_tag="ACOIAS_000174"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2699905.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62659..63930
                     /locus_tag="ACOIAS_000175"
     CDS             62659..63930
                     /locus_tag="ACOIAS_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGZ2699906.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63988..64587
                     /locus_tag="ACOIAS_000176"
     CDS             63988..64587
                     /locus_tag="ACOIAS_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2699907.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            64592..65497
                     /gene="dapA"
                     /locus_tag="ACOIAS_000177"
     CDS             64592..65497
                     /gene="dapA"
                     /locus_tag="ACOIAS_000177"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGZ2699908.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPSKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65603..66235
                     /locus_tag="ACOIAS_000178"
     CDS             65603..66235
                     /locus_tag="ACOIAS_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HGZ2699909.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            66713..69220
                     /locus_tag="ACOIAS_000179"
     CDS             66713..69220
                     /locus_tag="ACOIAS_000179"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2699910.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69562..71724
                     /locus_tag="ACOIAS_000180"
     CDS             69562..71724
                     /locus_tag="ACOIAS_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HGZ2699911.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNETTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRALNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71729..72346
                     /locus_tag="ACOIAS_000181"
     CDS             71729..72346
                     /locus_tag="ACOIAS_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2699912.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNKKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            72535..72879
                     /locus_tag="ACOIAS_000182"
     CDS             72535..72879
                     /locus_tag="ACOIAS_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGZ2699913.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            73449..74315
                     /locus_tag="ACOIAS_000183"
     CDS             73449..74315
                     /locus_tag="ACOIAS_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGZ2699914.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRIKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            74428..74832
                     /locus_tag="ACOIAS_000184"
     CDS             74428..74832
                     /locus_tag="ACOIAS_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HGZ2699915.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            74999..75448
                     /locus_tag="ACOIAS_000185"
     CDS             74999..75448
                     /locus_tag="ACOIAS_000185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2699916.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(75604..75981)
                     /locus_tag="ACOIAS_000186"
     CDS             complement(75604..75981)
                     /locus_tag="ACOIAS_000186"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2699917.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            76252..77160
                     /locus_tag="ACOIAS_000187"
     CDS             76252..77160
                     /locus_tag="ACOIAS_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HGZ2699918.1"
                     /translation="MNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKCMNV
                     PIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSINAF
                     IKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINRLPV
                     LIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDIETL
                     NTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKYYLE
                     KTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            77644..79062
                     /locus_tag="ACOIAS_000188"
     CDS             77644..79062
                     /locus_tag="ACOIAS_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGZ2699919.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            79138..79950
                     /locus_tag="ACOIAS_000189"
     CDS             79138..79950
                     /locus_tag="ACOIAS_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2699920.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT        30684 a         9425 c        13359 g        26711 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ctttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa atacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttaacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat ctctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 acaatacttt tattttagtt ccttctattt caactatatc tcctgatttt atcttttttc
     7801 ctcttcttaa ttctatttca tcatttactg tcactaaacc ttcttgaatt aaaaattttg
     7861 catgacctcc tgttgatgca atctctgcta atttaagaaa ttgatccaac tttatatatt
     7921 ctgattctat agttatttca gtcataatag ttcctccttg tcactatttt aaacaaattc
     7981 ctattgatac actaaaagta tattatacca taaatatgct tataaaagga gttgtctaag
     8041 attagaaaaa ttttctacaa aagacaactc cttcatgtct gctatatatt tgatgatatt
     8101 cttactggaa gcagtaagta aatataatta acatcatctg ttggtttaat tatacaagga
     8161 tttacatttg tagtaaattc tataaatatt tcctcattat ctatattttt caaaccttcg
     8221 ataaaatatc tagaattgaa agctatatct aggtaatctc catctaaatc tatttcaact
     8281 tcttcataca catttccttt ttctgtatta gaagtaattg ccattacttt atctcttata
     8341 gataacttta taagattatt tttttctgat tgagacaata aagacgctct ctctatacta
     8401 tttaaaagtt ccttagtatt taatttaact ctgctgttat gttctcttgg caataatttt
     8461 ttataatcaa taaaatctcc ttcaagcaat cttgtaataa ttttcgtatc attaattata
     8521 aaaatagcat ttttttcgtt aaatccaact ttaacattat cctctccaga aagcaaacta
     8581 tttacatcta taagtgtctt tccaggaatt atgactttta tattttcagt taaactatca
     8641 accgaacaac tttttacagc taatctatat ccatcaattg ctactaaatt taagtttcta
     8701 tctacaattt ctaaaagttc tcccattaaa acaggttttg tttggtcctg tgagatagca
     8761 aatacagttt gtttaatcat attttttagg atttcttgag gtatgctata taaatcttct
     8821 tcatttaatt ctggtaattt aggaaattct tttgctgcat aacctttaat tttaaatctt
     8881 gaatttacac aatttatgta aatattattt tctgaatcag tttctatctc aacaaaggaa
     8941 tctggtaatt ttctaattat atctccaaat agccttgcat ctacaacaac atcacctttt
     9001 tctatgattt ctgcttgtac ataagtttct attcctattt cagcatcata tccagtaagt
     9061 tttaattgac cttcttctgt agatataagt atacctttta ataattctat tgttgttttt
     9121 ccattaatag ctttttgagc aattccaatt ctatttgcta ggattttttg attacaaatt
     9181 attttcaatg tggataacct cctctggctt tttatatata aaaataagtt aaaatcatag
     9241 tagtaatagt agtaggtgtt gttgataatg ttaataagta tgtataacat tataaataca
     9301 aagatattaa catgtgtata agatgttaat atctttgtta ttttagactt gataatttgt
     9361 taataatttt tgttcaaaaa aagtattaac aggttattca agagatataa acatacaatt
     9421 gtgtataaat tatcccttca aatctgatat aattttgtct attttagtct taatttcttc
     9481 actttcttct atatccttag atactttgtc atgagcatga attactgttg tatggtctct
     9541 acccccaaac tcttcaccta ttttaggaag ggataaatca gtaagctcgc gcgttaaata
     9601 catagctatc tgtcttggat aagctattga acgtgttctt tttttagaat taaaatcttc
     9661 catctttaaa ttgaatacag aagatacttt ttcttttatt ctgagaacat ttatttcttc
     9721 atttttagaa gttgttataa tatcttttaa agcttctgta gcaagatcaa aagatattac
     9781 cctattgcta agagaagaat aagcaaccac tctagttaat gcaccttcta gttcccttat
     9841 gtttgactta atattttttg ctatatatga cataacctca tttggcacat ctatacgttc
     9901 tagttgagcc ttttttctaa gtatagcaat tcttgtctca aagtcaggtg cttgaatatc
     9961 agttataagt cccatttcaa atctcgatct tagcctatct tctagtgtag gtatgtcttt
    10021 tggcggtcta tcactagata taattatttg tttgtttgct tcatgaagag tattgaatgt
    10081 atggaaaaac tcttcttgag ttctttcttt tcctgctata aattgaatat cgtcgattag
    10141 taatacatct acgtttctat atttattcct aaattcttcg tttttatcgt cttttataga
    10201 gtttataagt tcattagtaa atttttctga agaaacgtaa actacttttg agtctttttt
    10261 ttgactaaca atatgatgac ctatagcatg cattaggtga gtcttaccta atccaactcc
    10321 tccatacaaa aaaagtgggt tataagcttt agctggagat tctgcaactg caacgcatgc
    10381 agcatgtgca aatctattac tattaccaat aacgaatgta tcaaatgtgt attttggatt
    10441 tagattgggg taaagaactc tataatttaa atctgtgcta tcagaattta aatcagcaac
    10501 ctctttttcg ctcaatacaa atttaatatt atattttttt aatgacagtt gactaatagc
    10561 atcttcaatt aaatgtaaat atctattctc taagatatct ttattaaaat cactaggagc
    10621 cagtaagatt aaatcattaa gatgtatttt taatggtacg atgtttttaa aaaaggtatt
    10681 aaaacttact gaagttaagt caccttttat taattgtagg gttttgtccc ataaagaaac
    10741 tatatccata atataaacct ccgaacagaa taattaatat tttccacaaa gttattaaca
    10801 gaaaagcaaa aatatatata aattttacag aataactaaa tttatggata atggtgttga
    10861 taaattaata atagtataaa ttattaaaaa aatatgaaaa gatataaaca atatgtagat
    10921 aaagttataa ataacataaa catacaataa atggtgatta taactagagt attgtcagta
    10981 atcttaaaag ttatacacaa aagtatccac aagctgtgaa taatttctaa taagttgata
    11041 acatttatgc acattatcaa caagtatatt tataatatca aaatatatgt caaacttcaa
    11101 tgatttgtaa ctaatttatc cacaatatta actaattgtt gataaaaata taaacatgtt
    11161 aaaattactt tatttgtgta tataattgaa aaaaataatt tagaataaaa ttaaatagat
    11221 ttttactagt attttcaata aaaaaaatac ataatatata tatggtagat tttgtgatat
    11281 aagcgtccca aaaactcgtt gacaacagta gtaaaagtat ttataattat atgtgttatg
    11341 tttactttga gggtaaagtt attattttta taaacggaaa ataaaatctg aaaggcggtg
    11401 caaaataatg agtaaaagaa cttatcaacc aaaaaagaga caaagaagta aagaacatgg
    11461 tttcagaaaa agaatgaaga cgtctaacgg aagaaacgtg ttaaagagaa gaagagctaa
    11521 aggaagaaat agattaactc attaattaca aaagggccgc tgcaaaggtg gcctttttgt
    11581 gtaaataatt acaattcata gctgtgttta tggaggttaa ctatggactt taataggact
    11641 aaagggttga aaaaagactc tgattttaga aaagtatata aacacggcaa atcttttgca
    11701 aacaaatatt tagtaatata tatacttaaa aataaatcag attatagtag agtaggtatt
    11761 tctgtttcaa aaaaagtagg aaaagctatt actaggaata gagtaagaag attaataaaa
    11821 gaagcctaca gattaaatat tgatgaaaaa attaagcctg gatatgatat agtatttata
    11881 gcaagggtat ctagtaagga tgctactttt aaagacatag acaagtctat aaaaaattta
    11941 gttaaaagaa cggatatttc tatttaattg tatgctataa atatggaggt taatgacttt
    12001 gaataaaata atttatatat taaaggaaat aagtaagcat ttatcgaatt tatgtattta
    12061 tttagtgaga ttttatcaaa agtatatatc tcccttaaag ggacctacct gtagatttta
    12121 tccaacttgc tcacaatatt caatagaggc gtttaagaaa tatggatttg taaaaggtat
    12181 gtatttaacg ataagaagac ttttaaaatg tcatcctttt catccaggag gttacgaccc
    12241 tctaaaataa aagagagtaa ctattaggag gtataaaaaa attgaatata ataggaaatt
    12301 tcttaggatt gattcttaag gtaatatttg aatttgttaa tcattatggt atttctataa
    12361 tactttttac gatacttgta aagattattt tattaccatt aacaattaag cagactaagt
    12421 caacaaaggc aatgcaagat atacaacctc gtataaaaga gatacaagaa aaatataaaa
    12481 ataagccaga aaaacaaaat gaagaaattg ttaagttata tggagaagct aaaattaatc
    12541 cactatctgg ttgtttacct ttattaatac agtttcctat attaatagga ttatttagcg
    12601 ttttgagaga accagttgct catggcgttt ttgcaaacaa agctgcattt ttagcagctg
    12661 acaatgggtt tttatggata aaaagcttaa catcaccaga ttatgtatta gcagtatttt
    12721 caggagcaag tgcatatgtc atgcaaaaag taatgacacc aaaagatcag ttacaaggct
    12781 ctatgaaagt aatgacatat gtaatggcag gtatgtcttt ctattgggga tttatattcc
    12841 cagcaggact tacattgtac tggacagtaa gtaacttatt ctcaatagct caatattact
    12901 taataatgaa tcctttaaaa gctaaactgg ctgctaactc taaggaggaa aaagttaatg
    12961 agaaaaagcc taaaattaat aaaaagtaag agtaaagagg aagctataag taaagctata
    13021 gcagagttga atttaaaagc agaggatata gaagtagaaa tacttgaaaa tccaagtaaa
    13081 gggtttttag gttttatagg tgctaaagat ggtacttatg aaatttttgt tatcgagagg
    13141 gaagtagatg tagctaaaaa ttttatagag gtaatgttga aaaatgcaaa tgttgatgcc
    13201 aaagtcaatg tatctcaaaa agataattta atcatggttg atattgaagg caaagaagct
    13261 gcaagtttaa ttggtagaag aggggaaact ttagattcta ttcaattttt gactggatta
    13321 gctcttaata aaataaataa agattctcat acaagagtac ttgtagatat agaaaattat
    13381 agatcaaaac gagaagaatc tttaataaga tatgctaata aagttgctag agaagtagcg
    13441 aaaacaagaa aaactaaaaa attagattat atgaatccat atgagagaag aataatacat
    13501 tcagctcttc aaaatgacaa atatgtaatt acatatagtg aaggaaccga tccatataga
    13561 agattagtaa ttgagtgtaa aagataaagt caacctccta aacattaggg ggttttattt
    13621 tttaatatta ttaaatcatt aatggttggt taaatgtaaa tctatatgta aaaaattatg
    13681 aattaagagg tgataaattt gtttatagat gatacaattg cagcaatagc tacagcacct
    13741 ggagaaggtg gtataggtat acttagaata agtggagaaa aagctttgaa ggttgcagaa
    13801 gaaatcttta aatctatgtc tggaaagagt attgaagaat acaataagag aacattaata
    13861 tatggaaata tagtggataa tgaaaatgta attgatgaag ttttattggc atatatgaaa
    13921 ggaccaaatt cctatacagg agaagatgtt atagaaatta attgccatgg tggatttatt
    13981 tcggtaaaga agatattaga attaatacta tcaaaagatg ttagattagc agaggctggt
    14041 gagtttacta aaagagcatt cttaaatgga agaatagatt tatctcaagc agaggctgta
    14101 attgatgtta taaaagccaa aactgatata gctcatgaag ttgctcaaaa tcaattagag
    14161 ggttctttat caaagaagat aagagaattg agagataaag tcactgagat attagctcat
    14221 gtagaagttg ctatagatta tcctgaggaa gatattgagc atataacata tcaaactctt
    14281 aaagaaaaaa ctgatgaact taaaaaagac attaaaaaat tatatgatac agctgaaagt
    14341 ggaaaaatac ttagagaagg cttaaagacg gtaattgttg ggaaaccaaa tgtaggtaaa
    14401 tcttcactac ttaattctat tttaggagag aatagagcaa tagttactga tataccagga
    14461 actactagag atgttataga agagtttgta aacataaaag gtataccttt aaaaattgtt
    14521 gacacagcag gtataagaga tactgatgat attgttgaaa aaataggtgt tgaaaagtct
    14581 aaagaatcat ttactagtgc agatttgata gtaatggttt tagatgcatc tagaaagttg
    14641 tcagaagaag atatagagat attagaaaaa cttaaggata aacaaacaat agtgctgtta
    14701 aataagaatg atttgaaaca agagattgaa gaagaaaaaa tacttaaata tgtagaaaat
    14761 aatagtataa taaaaatatc agctttacaa caagaaggaa tagaagaatt gcaagataaa
    14821 atagagtcta tggtatataa aggtagtatc aaaaataatt ctagtttagt agtaacaaat
    14881 tcaagacata aagatgcact tagtaaagca tataaatctg caacagatgc tttaattgct
    14941 ttggaacaaa gtatgccatt tgactttgta gaagtcgatt taaaaaatat atgggactat
    15001 cttggatata ttaatggtga tacagtaaca gaggatttac tagataatat attccataat
    15061 ttctgtattg gtaagtaatg tacaaaagta aacttgtttt tattatagag atttttaaaa
    15121 aatttgaagt gtttctcatg aaacattaat tttgaaaaga aaggaattat aaatgataaa
    15181 gtttgaagca ggaaaatatg atgttatagt agtcggagca ggacatgctg gatgtgaagc
    15241 tgctttggct actgcaagaa tgggatataa aactttgata ataacaatgt ctttggattc
    15301 catagcatta atgccttgta atccatcaat tggagggact ggtaaaggtc aattagttaa
    15361 agaaatagat gccttaggcg gtcagatggg attaaatata gacaagacat atatacaaag
    15421 tagaatgtta aatactgcca aaggtccagc ggtacactct ttaagagcac aagcagataa
    15481 atttaaatat catgaagaaa tgaaaaagac attagaagat gaaccaaatc ttgatatagc
    15541 tatggatgaa gttgtagaaa tacttcatga aggaaatgtt gtaataggag ttggaactaa
    15601 actaggatgt tcatttaaat ctaaagctgt aatattggcc actggagttt atctgaactc
    15661 aaagatatat atgggtgaag ttgcatttta tgaaggtcct aatgcattag ggtatgcaaa
    15721 atatcttaca gatagcttag ttgagcttgg ccttagaatg agaagattta agacgggaac
    15781 tccagcaaga gttcatagag atagtataga cttctctgta atgtctttac aagagggtga
    15841 tgaaaaagta actccattct catttatgaa tgaaaatata gaaaaaaaac aagaaccttg
    15901 ttatttaaca agaactacag aagaaactca aaaagttata ctggataatt taaaaaggtc
    15961 tgctatgtat agtggagtaa tagaaagtac tggtccaaga tactgtcctt ctatagaaga
    16021 taaagtagta agatttagtg ataaaacatc acatcaatta tttatagaac cagaagggtt
    16081 aaatactaaa gaaatgtata tacaaggaat ttcaactagt ttaccttttg aagttcaatt
    16141 agatatgtac aaaactataa aaggacttga aaattgtaag ataatgagac ctgcatatgc
    16201 tattgaatat gattgtgttg acccaactca actaaaaata agtttggaaa taaagggtgt
    16261 tgagaactta tttagtgcag ggcaatttaa tggaacttct ggatacgagg aagcagcagc
    16321 tcaagggctt atggctggta taaatgcagt tagaaagata gaaggaaaag agccatttgt
    16381 attagaccgt tcagaagctt atataggtgt cttattagat gaccttgtta ctaaaggaac
    16441 taacgagcct tacagaatga tgacatcaag agcagaatat cgactatatt taagacaaga
    16501 taatgcagat atgagattaa ctcaaaaagg ttatgatata ggattagtta aaaaagatag
    16561 atatgaaagg ttcctaaata aaaaggctgc tgtagagaaa gaatttgaaa gactaaaaaa
    16621 tgaaagagtt acaccaaaag aagtaaatag tttgttggaa gaaaaaggag caacaccaat
    16681 aaaggttgga atatctctat atgaattttt aaagagacca gaggttactt acgaattgct
    16741 tgaagaatta gggaaaggcg ctggagaaga tgtttcaaga gaagttaaag aacagtgtgt
    16801 aataataact aaatatgaag gttatataga gaaacaattg aaacaaatag accaatttaa
    16861 aaaacttgag aataaaaagt tggatgagaa aataaattat tcatctatag aaggtcttag
    16921 attagaagct agacagaaat tagatgatat aaaacctatt tcaatagggc aagcatctcg
    16981 tatatctggt gtttctccag ctgatatatc agttttactt atatacctag aacaaatcag
    17041 aagaactaga ggtggcaaag gtgaataata tagaacttct gaaaaacggg attgaaggtt
    17101 ttaatataga tacgaatgac agtatgttag aaaaatttaa aatctataga gagatattag
    17161 ttgaatggaa taaaaagatg aatcttactg gaatagaaga tgaaaaagaa gtgtatataa
    17221 aacatttttt agactctatt tcagctgtaa aaaatggata tataaaaaat gggatgtcta
    17281 taatagatgt tggtacaggt gctggttttc ctggaatacc attgaaaata tgtttagata
    17341 gcttagaact tactttgtta gattcgttaa ataaaagaat aaacttttta gaggaagtta
    17401 gtagagtact agagttagat aatattacat ttatacatgg aagagctgaa gattttggta
    17461 aaaatgaaga ctatagggaa aaatatgatg ttgctacggc aagagctgtt gctggattac
    17521 cgattcttat ggaattttgt gtaccatttg taaaagttgg agggtatttt atttgcttaa
    17581 aaggtccgaa tgctaattta gagctagaag aatcacaaaa ggctattgat gttttaggat
    17641 tggagtatat tgaaaaaata gatgtagagt taccagaggt agatttaaat cataatatat
    17701 tagtttttaa aaaagtgatg gaaacaccag ttaagtaccc tagaaaagct ggtaaaccag
    17761 ccaaaaatcc tattaagtaa tgaaaaattt attattggta agcttaatta tttctcagga
    17821 aataattaag cttactgtaa aaaaacaaaa ataaaggaat tattaaagta gatgcagaaa
    17881 taaatttgta tgtacatata gagaacaaaa ataatttata tctaaaataa gagaggtatg
    17941 ttatggaaga taaaagagtt atggagatac ctatagaaga tgtagttcca aatccatatc
    18001 aaccaagaaa aatattttca caagtttcat tggaggaatt gagcaattct ataaaggtat
    18061 atggtataat tcaacctata acagttagag caaaagatgg caaatatgaa ctaattgcag
    18121 gagaaagaag attaagagca gctaaattag cagagcttaa aaccatacca gcaattataa
    18181 ataatatgaa cgatgaatct tctgctgttc tagcgttatt agaaaatttg caacgagaag
    18241 acttaaattt cattgaagaa gcaattggtt atgaaaactt aataaaagaa catgctttta
    18301 ctcaacagca attagcagaa aaattgggca aaaatcaatc tactgtagca aataagctta
    18361 gaatattaaa gcttccaaac gatataaaaa tgaagctaat agagaataat ttaacagaaa
    18421 gacatgctag agcatttctt aaactaccaa gtgaagattt aatgcaaagt gtgttagaca
    18481 aagtcataaa aaatgaattg actgttaaaa aaacagaaaa attgatacaa gatgtattag
    18541 aagaaactaa agttcaagaa gagcctgata aaaaacagaa tataaagggt gctatgagta
    18601 taagaattta cataaatact ataaagcagg cctttgatgc tatatcaaat acaggaattg
    18661 atgctaagta taatgaaata gataagggag attatatgga agttgttgta aaaatcccta
    18721 aaaaatagag attactgctt accagtaatc tttttttgta atataagcct tgtaatataa
    18781 attgatttat agacattatt tatacttaaa aaacacctat tcaacattta aaattaattt
    18841 aattcttaaa ggggggaaca tcatgggcaa agttatagct gtattcaatc aaaagggtgg
    18901 agtcgggaaa actacaacaa atgtaaattt aagtgctagc ttaggaactt tgggaaagaa
    18961 aatattagta ttagatttag acccacaagg aaatacaact agtggatatg gtattaataa
    19021 aaacgaagta gaaaatacaa tctatgaaat tatgttagat gggttacata ttaaagaagc
    19081 aataataagt actgaatttg agaatgtaga tgtagttcca tcagctactg aactatcagg
    19141 tgcagagata gagcttacat ctaaaactaa tagagagtat attttaaaaa attccataaa
    19201 agcggtaata gatgaatatg attatatatt tttagattgt ccaccctcac tggggatgct
    19261 tacaataaat tgtttaactg ctgtagatag tgttttaatt ccaatacaat gtgaatatta
    19321 tgctttagaa ggtgttagtc aattaatgga gactataaaa ttagtaaaat caagattaaa
    19381 tgctgatata gaaattcaag gtgttgtttt aagtatgttt gatggaagag ctaatttatc
    19441 aatacaagta gttgaagaag ttaagaaata ctttaaaggt agtgtatata caactttaat
    19501 tccaagaaat gtaagacttg cagaagctcc aagtcatgga aaacctgtaa tttattatga
    19561 taaaagatgt agagggtctg tagcttattt ggaactagca gaagaattta tagatttaga
    19621 agaggaggaa tggtagtatg gagaataagt ctaaaagaag taatagatta ggtagaggtc
    19681 ttagtgcatt gataccagaa attaaaggag aaacttcaga aaaagaaata gtcaacatag
    19741 atatagacaa aatatatcca aacgaagttc aacctagaaa gcaatttgat gaagaaaaga
    19801 taaaagtatt gtcggattct ataaaaaatt atggtgtttt acagccaata gtggttaaaa
    19861 tggatgaaaa taataaatat atgataatag ctggagaaag aagatttaga gcatcaaaac
    19921 ttgcaaataa aaatcaaata cctgctataa ttaaagatat agatatgaaa gatataatgg
    19981 agatagcctt aattgaaaac ttacagaggg aagatttaaa ttcaatagaa gaggctctag
    20041 catataagag tttaatagag cattacaatg taactcagga agaaatatca gaagctgttg
    20101 ggaagagtag acctcatata actaatacac taagactttt aaatcttgga caagatgtta
    20161 tagaaatgat agacagtggt agaattacag caggccatgg taaggcttta ttgagaattg
    20221 cagataaaga tttgcaactt caaatagcga aaaaaataga agaagaagaa ctttctgtaa
    20281 gagaagtcga aaatatagct aaaaaaatat ctgaaaataa acaagaagaa cctaaaaaat
    20341 ctaaacctaa ggatgtattt attttagatg ttgaagataa acttagaaat atatttggaa
    20401 caaaggtgaa tatttctaaa ggaaagaaaa aaggtaaaat agagatagaa tactataatg
    20461 atgatgattt aaatagtatt gtatcaatgc ttcttgaata gaacaaaaat ataaaacaga
    20521 gaggaagatt gttatgattt atttagataa tgctgcaact acatatccaa aaccagaaag
    20581 agtgtataat gctgttttag attgtatgaa aaattattgt gcaaatccag gtagagcagg
    20641 ccataaactt gctatgagag ctgctaggga gatatatgat acaagagaga atatagctaa
    20701 gttgttcaat gtaagtaatc ctatgaatat agtatttaca tctaatgcaa cagattcttt
    20761 aaatttagcg ataaaaggtg tattacaaga aggagaccat gtaataacaa cgtctatgga
    20821 gcataattca gtaataagac ctataaaagc attggaaaaa aggggtattg aaaatactgt
    20881 agtaaaatgc gattatgagg gatttttaga ttatgaagat ttagaaaaat caataaagaa
    20941 taatactaaa ttgattgtaa caacacatgc gtctaatgta tgtggaactt taatagatat
    21001 aaaaaaagtt ggagaaatag ctaaaaaaca taatatacta tttttagtag atgcatcaca
    21061 aacagcaggt gtttatgata tagacgtaaa tgaatgtaat atagatatgt tggcaatgcc
    21121 aggtcataaa tgcttatttg gacctcaagg aacaggtata ttatatgtaa gagaagggtt
    21181 gaacttaaat atattaaaag aaggtggaac aggtagtaaa tcagaagaaa tagttcaacc
    21241 agagttattc ccagataaat atgaatcagg aactcataat actccaggta tagcaggtct
    21301 taaccaaggt atactattta tatttgaaag aggaatcaac aatataagac aacatgaaga
    21361 agaattatgt caatatatga tagacaaatt agaggaagta ccagatataa agatatatgg
    21421 accaaaggac agtaaaaaaa gagcatcagt aatagctcta aatataggag atatggattc
    21481 tggagaggtt actttcttat tagatagtga ttataatata gcgactagat caggaataca
    21541 ttgttcacca ctagcacata ccactttagg tactttaaaa caaggtgcag taaggttcag
    21601 tataggatat ttcaacacaa aagatgagat agataaagca gtggaagctt tgaaaaaaat
    21661 ttcaaaaaat aaatagcatg agatttaata atatagaaat aaaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggttc ctaagaacct
    21781 ctaatctatt tgataatagt ttcaagaaat tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 ggtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagagtaa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa atttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtatttat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttatg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattaaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaacca ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagaatct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaata taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagagcaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt atagggccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttga cttacattga ttttttatta agttaaagct
    34921 acttatacat attctaactg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 ataatattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taaggtacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag attctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aggtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcacttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacttt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tcttcattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag aaacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacataga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagctga aaacgttaga gattttgcat ttatactaag tagtaaattt
    38821 gatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagtga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcttt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aatagtgaat gtaagtaaat tttgtgcaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aaaaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttaa
    40261 gcaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aactgctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtggtaatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac cgttacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt taagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gagcttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctttagggct ttctgatgag gttttaggaa gggctgatta taggttatcc
    42961 ttttctaaga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gtttttgtta
    43081 atttgacact taataatatt aaaataacaa aaaagtattg aaagtatatt gcatttaagg
    43141 tagaattaaa taaaatatta taacattata atgtttatat ggttgttaat aaaaatttgg
    43201 aggaaaatat atgataaaaa taatgttggc ctgttcagca ggaatgtcta caagtttatt
    43261 agttactaaa atggaaagtg cagctaaaga aaatggtata gagtcgcaaa tttgggcaat
    43321 accagaatca actatacaaa atgaaataga aaaatgtgat gtattattac taggaccaca
    43381 agttagatat gtattaccta aggctcaaga aattgctaag ccatataata taccagtaga
    43441 agttataaat atgatgcact atggaacagt taatggagaa gctgttttaa atagagcgat
    43501 agaacttaac aacactaagt aatataaatt ataatgtatt tttaatatat aaattgggag
    43561 aatcaaagga tggatgaaaa gactatagaa atatctttta atataatagg ttatgcaggt
    43621 gaaggtaaag gattagcttt tgaagcaata aaagaggcta agaatggaaa tatagaaaaa
    43681 gcaaaagaat tattaaaaga gtccagggaa gttataaata aagcacatag atatcaaaca
    43741 gaattaatac aaaacgaagc tagtggaaac aggactgaaa taagtgtcat acttatacat
    43801 gcacaggacc acttaatgaa tggcatgaat tttcaacagt tagctgagga aatcgttgat
    43861 ttacatttga aattacaaga taaataaaaa ttaaagataa taaattaaga gagttcttaa
    43921 aagatacatt atgtgtagca cactaattgt tagattattg attcaatatt tagtgagtaa
    43981 tacattagga ttattttgag gactcttttt atatttagtt taaagttaat aaatatttat
    44041 tattaataat ataagattat atgttataat attataacat ataatttaag aggtgaatca
    44101 tgttaagaaa tcaaccatta tatatacaag ttgtagatat attaaagcaa aaaatattaa
    44161 ataacgaata taaaattgat gaaaatattc caacagaaag ggagttagag caagagtttg
    44221 gagtcagtaa aattactatt agaaaagcta ttgagatatt agaatcagaa gggtatgtag
    44281 agaaaaaaag tggaaaaggg acaaaagtaa taagtaatag tctttttaat aagctatcaa
    44341 aagcagagtc tttttcaagt atacttttat ctaaaggtca taagttatct aaaaagacta
    44401 tttcaattga aaaaataata aatgatacaa attcacaagt atttagtata tttggagaaa
    44461 aatgtagcaa gattactaga atgtattact tagatagtaa gccatatata ttttttactc
    44521 attatattcc ttttgatgaa aatctaactg ttgaaagttt taataatgaa agttctttat
    44581 atatgtactt atataggaga aatataaata taactagatt tgaagatgaa ttttttatag
    44641 aagaatcaga actagaggta tcaaaggctc taaatctagc taaagacata ttattaggaa
    44701 gaaaaagaaa gacctatgat gagaatgata atatagttga gctatcattt gcaagatata
    44761 atacagaagt acaaaactat gtaattaaat acgaaatata ataagaatcc tgagtttatt
    44821 gaaatgataa tatgctcagg atttttgttt agttgtaaaa ataaaaaata ttataacatt
    44881 ataatgttta caactaaaca ttataatgtt ataatatttt taataaacaa gtgatattat
    44941 caattacata aaaattagtt tatgttatga aatcgttatt gcattataaa taaaggataa
    45001 tatcaataat acagggggat aactatggaa aaattcgcaa gttttttaga gcgcaagatt
    45061 atgcctgtag cagcgaagat aagtaatcag agacatatga gagctgtaag agcaggtata
    45121 atagcaacat taccactaac aatagtgggg tcatttttca caatattatt aaacatacct
    45181 attgatgctt atatggattt tatagctcca tttaaagcaa tattagatat accatttaga
    45241 tatactgtag gatttttatc tctatatgct acttttggga tagcatcatc acttgctaaa
    45301 agctacgatt tagatacaac aggtgttggt atgcttgcag ttatggcatt tttagtttct
    45361 acaatagttc caactcaagt agttgaacca gtaggtggag ttatagaagc tggtagatgg
    45421 atggatatgg caaaacttaa ttcgccatca ttatttggag ctatcgtaac atcattaatt
    45481 acagttgaaa tatatcgttt tatgaaggaa aagaatatta caattaagat gccagcagga
    45541 gttccagtag aggtttcaaa ttcatttact gcattattac cgacagcagt aattttaatg
    45601 ttattctggg taataagaca tattttagga tttgatataa gtgttgcact aagcgctatg
    45661 ttatcacctt taaaaggatt cttatcagga aatagtttag gtggtgggtt actgacagta
    45721 ttcctaataa ctttattctg ggtacttgga atacatggtc cagctataat ggggccagta
    45781 ataagaccta tatgggacat gtctatagca gaaaatataa ctgcattcac agaaggagta
    45841 tcagctcatg caatgccaaa catatttaca gaacaatttt tacagtggta tgtatggata
    45901 gggggagcag gggctactct tgcattagtt gtaatgttta tgttttctaa gtcacaatat
    45961 ctaaaaagtt taggtagatt aggattttta ccagggttat tcaacataaa tgaacctata
    46021 atatttggag caccaatagt tatgaatcca atactaggga taccatttat aattggacct
    46081 ataataatga caattctatc atatgttctt actattacag gggttatacc tatgatggta
    46141 gcaagattac catttgcaat gccagcacca atagcagcag taatgagtac taactggagt
    46201 atattagcag gtatcttagt attaatcaac tttgtgatat catttgctgt ttattatcca
    46261 ttcttcaaag tttttgaaaa acaacaatta caaagagaac aagaagaaat gagtgaaaat
    46321 ttataaaaat acatagatat agaggttttg tactatataa aatatagaaa aaacttataa
    46381 gttattaagg gtgaaggtag gaggaatgac ctttgccttt aattttatat attttaaata
    46441 atgtaatttg aggaggagaa agatggaaag aagattagga atatcagtat atccagagca
    46501 tagtaacatg gaagatgata aggcatatat aaaaatggct tcaaaatacg gatttaaaag
    46561 aatatttatg tgcatgcttt caattacaag acctaaggaa gaagttaaaa aacattttaa
    46621 agagattata acctatgcaa gagaattagg atttgaagta atccttgata tagctccaaa
    46681 catatttgat atattacaaa tatcttatga tgatttaaca ttttttagtg agttaaatgc
    46741 tagtggaata aggcttgact atggatatga tggaagtaaa gaagctatga tttctttcaa
    46801 tccatatgat ttaaaagtgg aactaaatat gagtaatgat gtagcatact tggataatat
    46861 aattacttac aagccaaatg taaataatat atatggttgt cacaattttt atcctcaaag
    46921 gggtacaggc ttgccatatg atttttttgt aagatgttca aatagattta aaaagtatgg
    46981 aataagaact gctgcttttg ttaattcaaa atatgcaaca atcggacctt ggaatataaa
    47041 tgatggactg tgtacgcttg aaatgcatag aaacttagat attgctacag caactaagca
    47101 cttattcgca acaggattga ttgacgatgt aattgtaggg aatgcatatg caagtgaaga
    47161 tgaattaaaa agaatgtctg aagtaaataa atatcaagtt gtatttaatg tagtaactga
    47221 ggaaaaaact tctgaagtag aaaagacaat aatggcaaat gagcaacact ttagaagagg
    47281 ggatataacc aaccaagtag caaggtctac agaagtaaga aaaaaatatg ctaaagaaaa
    47341 tttccctaag catgataata cgaccatctt taaaagagga gatgtagtga ttggtaatga
    47401 tgactttgga aagtataaag gggaattaca gttgatactt caagattgtg cagatgaaag
    47461 aaaaaataag gtaggtagaa taaaggaaga cgaaataatg cttttagact ttattgaacc
    47521 ttggagtaaa tttaaactta ttttagaggg agagtaaagt gaagatagac atattaataa
    47581 aaggtggaaa aacagtagat aaaagtataa ttgatatagc tatattagac gataaaataa
    47641 tagaagtaaa aaatcagata gatgattcta agtatcaggc aaatcaaatt ataaacctag
    47701 atggagaaaa atttattagt gctggttgga tagatataca tacgcattgc tacgaaagac
    47761 tagaccttta taaggattat ccagatgagg ttggaataaa aagcggtgtt actactgtag
    47821 tagatgcagg aacaacagga gctttagata taggtaaatt ttatgaagat actaaaaaat
    47881 ataaaacaaa tgtatatgct ttaataaata ttgctaagca gggaataaca tctcaagatg
    47941 aactttcaag tatgatgaat attgatgaat atgaacttaa aagagcagtt aaaaaataca
    48001 aagattttgt tgttggaatc aaagctagaa tgagtaaaag tgttgtaatt tcaaatgatg
    48061 ttgaaccttt aaaagtagct aaaagaataa aaaatgaact taatctacct atgatggttc
    48121 actttgggag ttctcctcca acgatagagg atatatttga ctatatggaa aaaggagata
    48181 tattaaccca tatatataat ggaaagccaa atggaatact tagaggcaat gaggttaaaa
    48241 aagaaatcat agaagctaga gaaagaggta taatccttga tgtaggtcat ggaaccgaaa
    48301 gttttagtat ggatattgca atgaaatcta aggatgcagg aatttttcca gacactataa
    48361 gtacagatat atatattaaa aatagaataa atggtccagt gtataatcta tctacaacaa
    48421 tggaaaagtt tatatatatg ggctattcac ttgaagatat aatagataaa gttactaaaa
    48481 atgcagcaga tgcaataagt ttaaaaaata aaggtttaat aaaagaagga tatgatgctg
    48541 atttaactat atttgatgtg gttaatgaag aaaaagaact tcaagattct ttgaataagt
    48601 cagtaatcac aagtacgtct ataaagccaa gagcagtagt agtcaatgga gaatatttaa
    48661 atataggtaa gtctatagga gagaatgagt agaagggaga aactattgta tgagtagtat
    48721 atttgaaaaa tactctgtaa gacgtgttat aaatgcttca ggcaaaatga ctatactagg
    48781 agtatcaaca gtagataagg aagttattga agcaataggt gaagggtgta aaaatttctt
    48841 tataatagaa gaacttataa ataaaacagg tgaatatata gcaaatttat tagggtgtga
    48901 aagtgcacta atagtttcta gtgcatctgc tgggattgca cagagtgtgg gagcaatgat
    48961 agcaaaagat gatatgagtc tagtttacaa tgtaaataat cctaagaaac aagttaaaag
    49021 ggaaataata attccaaagg ggcataatgt tgattatgga gttccagtag aagtaatgat
    49081 aagacttgga ggaggagaag ttgttgaggc cggctatgct aatatgtgta acaaggaaca
    49141 tataataaga gagataaatg aaaatacagc agcaattatg tatattaaat ctcatcattg
    49201 tgttcaaaag agtatgctaa gtgtattaga tgcagtacaa gttgctaaag aatataattt
    49261 acctttgata gtagatgcag cagcagaaga agatttaaat gtatattatg aattgggagc
    49321 agacctagtg atatacagtg gtgctaaggc tatagagggt cctagctcag ggttagtaat
    49381 aggtaagaaa aaatacatag ataatgtaaa gcttcaatca aagggtatag gaagagctat
    49441 gaaaatagga aaagaaaata ttgtaggtct aacatgtgca attgagagat atataagtaa
    49501 tgataaagta actttaaaag aaatggagga caagctaaat ccatttatag acaagataaa
    49561 tactataaag ggagtgagtg ctagtataac tagagattca gcaggtagag aaatacttcg
    49621 tggagaaatt gactttaatg aagatataat aaataagtca acacaacaaa ttataaatga
    49681 acttagaagt ggagagatag ctatatacac tagagactat aaggctaatg aaggcaagat
    49741 tgagattgat ataagaagtg taactggaag tgatttagat actatttata ataggataaa
    49801 aaatatagta gaaagttaga gtgtagagta agtgacatta taatgattta ataaattaaa
    49861 ttttaccaat ggaggggata aatatgaatt gtataccaca atattataaa aatagagttt
    49921 gtttaaatgt tcttgcagga agcataaaaa atgctaaaga agtatatgat gcttgtgatg
    49981 ggtatgtatt gataggagta ctatcaaaga attacagtac tgtagaagca gctattgaag
    50041 atatgaaaaa atactcaaag gaaatagata atgcaatatc tgtggggctt ggagctggag
    50101 acccaaatca gtcaaatatg gtatctcaga tttctaaaga acttcagcca caacatataa
    50161 atcaagtgtt tacaggtgtg gctacgtcta gagcattgct tggtcaaaat gatagtataa
    50221 taaatggatt agtttctcca actggtaaac caggatttgt aaaaatatcc acaggtccat
    50281 taagttcaaa agaaaaagat ggtatagttc ctatagaaac agcaatagct atgatgaaag
    50341 atatgggagg aagctcaatt aagttttttc caatgggagg gcttaaaaca aaggatgaat
    50401 atatagaagt tgcaaaagct tgtgcaaagc ataattttta tttagaacca acagggggta
    50461 tagacttaga taacttcaaa gagatagttc aaattgcact tgatgctggt gtagaaaaag
    50521 ttatacctca tgtttataca tcaataattg ataaagaaac aggtgaaacg aaagtggaag
    50581 atataggagt actttataaa ataatgaaag aattattggg atagatttat gaatatatta
    50641 gcttttggtg aaataatgat gagactttca gtacctgact acaagttctt aacacaaacc
    50701 aatgaactaa attatataat tacaggcaca ggattaaata tattatctgg acttaaaaac
    50761 tttggataca atacctatat gttgacaaag ttgcctaata ataatgtagg taaagcatca
    50821 agtgcaaata taagaaagct tggagtaaaa gatgacttta tcacatatgg agggaatcat
    50881 ataggagtgt attttcttga aaatgggtat ggagaaagac cttctgaagt gacttatttg
    50941 aataggttaa atagttcatt ttgtgagagt aaattacaag attatgatat tgataaatgt
    51001 ttagaagaaa tggatatagt ccttatttgt ggtattgcta tgcaacttac agattcaatt
    51061 aaagagattg ctttagaact agctagaaaa tcttatgaaa aggggataaa agtattcttt
    51121 gattttaatt ttagacaatc attaaatcca gaaaggactt atgatgattt agtaaatgat
    51181 tataagaaaa tactaccata ttgtcatggt ctatttgcta gttatagaga tataaataag
    51241 atacttaata tagatagtct aaatataaaa tctagttcag aattgattaa aaaagagtat
    51301 gatataggta ttgtagctgg aactatgaga agatttgatg atatgaataa tagatttata
    51361 aagggatata tatcaaatga tgatggttat tttgaaagta gggagtataa gttagaaata
    51421 tatgacaggg ttggtgccgg agatggatat gtagcaggaa ttatatattc atatttggaa
    51481 aatatgaaga gtgaagatat agttgagttt ggggttgcta gttcggtgtt agctcataca
    51541 acttatgggg ataattcttt agtaagcaca ggtcatgtac ttagacttat gaacaatgaa
    51601 aaaatagata tattgagata atacatttgt atataataaa atttttatct tttttagaca
    51661 cacttttgac ttgtttatat ttgttaaaca gagttatttt taatgattat aaatattaaa
    51721 ttaaaagtta aataataaaa tagtagaaaa tagcattaac tatagaaaaa atggttaatg
    51781 ctatttttat cgttaatatt gattaatttt atattataga aatgaacttt tctaatgctg
    51841 gatttgtatt ttttttattc catacaacta caacttcaat tctattttct acttctttta
    51901 taggtactat tgcaatatca cgttctatca ttgtatctgt aacacaaaat gaaggtaaaa
    51961 tggatattcc catgttagaa gataccaatg attcaatagt attaagatta tttgaacgtt
    52021 gtaccacatt aggtacaaac cctgcttcac gatatctatt ctgtatcatt gacttttgtc
    52081 catactcatc ccctgtttct tttacaaaaa taaatttatc atgttgtaat tcaattaaat
    52141 cgatagaatc ctcattgtaa agtggatgac caggaggtaa aagagctacc aaagaatatc
    52201 tttctacagt tttatattca aaatctttta atggttgatt ttctggatta ataacaaaag
    52261 cgaggtccat agaattattt tttactaagt ttaaaagttc ggttatttca ttagtacaaa
    52321 tatccataga tatattaggg tatgtattag aaaattcttt taaatattgt tgaagtttgt
    52381 ttttttcata tcctattaag gttccaatgc tcaaagttcc aaaaaaacca tgtgcaaatt
    52441 gttgtgtttt tgcaatagca tcattgatat tttttattat agtacgtgcc tcagataaaa
    52501 aaacttttcc agctggtgtt agttcaacat gacgtttact tcttgtaaat agagttacat
    52561 ttatttgttc ttctaaagct tttatttgct gtgtaacagc agtttgagaa atataaaatt
    52621 tttctgctgt tttggtaaaa cttaaatttt ctgctacaga tataaaatat tctaattgtt
    52681 tagtattcaa ataatcaccc ctatttaaac ttgattgttt gtaaacctta tttatctata
    52741 attaaagctt attatagtat aagaaaattt acttttaaag aatattagaa aatatttgta
    52801 taaaaatata tgttttaagt atatctttac attagatttt ataaatttat taataagaat
    52861 aacttatata ttccaatgaa aacgatattg gatttaagta agtttgtctc ataaaatgaa
    52921 agtatatcaa ttgataatac tttaaataag aagggagaaa aaatggaaga taaattttat
    52981 gcaaaaggca acggaaataa cggatatatt aaaaatcttg aagtttgttc ctttaataac
    53041 ttagatggaa cttgtggaat gtttcaaatg gctctgtaca aaagagatga aaaatactat
    53101 ttatatggat gctgttttgg aggaaataaa aaaaatggag taatgattag cgatattaca
    53161 gacccttata atccacaatt tataaaacat tttcaaatgt tagaccctaa agagtatcct
    53221 acaacaacaa ctcccaaaat tcaaatagca gatgatttaa tgatagtagc aatgagttgt
    53281 ggaagtggac caggagcact tgttgaccaa gctaaattag caaatattaa gtgtgaagca
    53341 ggaattagaa tatatagttt aaaagaagac cctttaaatc ctaagttttt aggatattgg
    53401 gattgtggct taaagcatgt aatgggtgtt catagattca tgtacaatgg tggaagatat
    53461 gtacatttat caagtgattg tgttggcttt gaaggtctga tttatagggt catagatata
    53521 ataaatccta ctaatccagt ggaaataggt aaatggtgga gaccagacca atatgcagat
    53581 ggatatccaa atagaacttt tgatgcagga gcacctcatt gcccagaatt tatggataaa
    53641 ggatggcttc atggacctcc atttgtaaga gacggaaaag catattgtgg ttatggagga
    53701 gctggtttag ttgtattaga tgttgaagat ttaacaagac caagatgctt aggtgaattg
    53761 ccatttatgc ctgcattttc tagtagactt gcaggtgcaa gaactcatac agcattacca
    53821 ttgccaggaa gagatttagt cgttgttcaa aatgagggag aaagattcca gttctttaaa
    53881 ccagataaca ttacagatgt tcaagctatg aataatatac atatggttga tgttagtgac
    53941 ccaacaaaac caacattaat tgctcaattt ccatatcctg aagttccaaa agatttccct
    54001 tatcctaact ttaatgttgc aggattagga aaaccagggc catttggtcc acataatctt
    54061 catgaaccaa tggataataa accatggtta gagcaaagag gagatagagt atattgctgt
    54121 tatttccatg cagggctaag ggtttatgat gtatcagacc catattatat caaagagcta
    54181 gcatatttta taccaccaaa tccaaataaa acaccagaag aatcttattt cccaggattc
    54241 ccaggaccac gcttggcagt aacagaagat cttatcgttg atgatagagg ctacatcatc
    54301 atagatgctt tagatgatgg attctatata ttaaaaatga aagaagatta aataaatact
    54361 aaagagggag agttgaaaat gaaaatacta ggtatttctt ttggaacaaa aaatggaaat
    54421 aatgacacaa tgtgtaaagt tgcacttaaa ggtgccaaag aagcaggggc tgaagtagaa
    54481 tttattcaaa tgtcatctat taacattaaa cattgtactg gatgttgtgc gtgtgttaaa
    54541 actttattat ctgggaaagg tagtatgtgt gtattaaaag atgattttga atggttgctt
    54601 gacaaaatga aagatgcaga tggtatagta gtttcagacc ctatttttga agaaggggca
    54661 tcaggattat tccatactat aatggaccgt tttggaccca gagcagatag aggaaataac
    54721 attataggaa caaaagttgc agaagctata ggaggaaaaa ttccagattc aagaatgctt
    54781 aatgataaag taatatcatt tatgggaata ggaggttctg actggggtac aagagttcaa
    54841 tgtgaacatg ctatgctagc tctcatacca atgtggaaag ttatagataa tgcttggttc
    54901 ccatgggcaa aggaattagt aatggatgat acaaggcttg ttcaagttca tcaaattggt
    54961 ttaaatatag ttgaagcagc aaaagatttt gaaaaagctt catatcaagg agaagaaggt
    55021 gtatgtccac attgccacaa tagattattc taccttgagc caggtactaa aaaggcaatt
    55081 tgtgctctat gtggtatagt tggagagtta gatactataa gtggaaaaac tatatttagt
    55141 tttccagaag agcaattagg tcatgctcat gatactttac caggtaagtt tatacatggt
    55201 gatgatatta agaaaatgga aggtcattat gcagaagttc gtaaaacaca agaatttaaa
    55261 gaaagaaaaa catcttataa ttttatagag acattaacta aagaaaaaca agctggagtt
    55321 tcattaggat aataattaaa atactaagac tcttttttaa gggatgttag atgttatagg
    55381 aggcttattg tatgaatgaa aagccaagtt ttaaacaaat tgaagttatt ttagcagtta
    55441 tttttttggt acttacatat tacaaggtta gattaactcc agcatttatt gttatattta
    55501 taccactata tttaagttat tttgcagtat ctatgattgt aaatcaaata gcaattaagc
    55561 tattggagat aaggcaagct agggaagcta ctaattacaa tataggaaat ggatagaaag
    55621 aaaaaagaaa tattaacact gtctatgggg attgctttac tacctccttt atgggcagta
    55681 ttagcacctt atataggaat aaaaactgga gctgtagcac taatctgtgc aggattatat
    55741 gtaactaatg gtaataaaca agaagatgga ttaaaaataa tgtttggttt ttggtgtgga
    55801 gatttatggg cagtgttagc cattttaatt atgggatata tgaatttcaa tcaaaaccta
    55861 gaattatttt taacacttag tatattaggt ttttttgcag ttgtaattgc atccttattt
    55921 gaaaaaataa ttttcctacc atcatggctt tgtggttggg ctataggtct aactattatg
    55981 acaggtgaaa atattattaa tttacaagat atttgtattc aaattgctgt agctatggca
    56041 gtaggagtat ggtacgtggg tgcaggtgta gatttgttta caatatatat tttgagtaaa
    56101 gataggaaaa agggaaattg aaagcatgtg aatatacttt gattatagga ggatggctat
    56161 gggttttgaa tttaaaataa tgagaagttt aatatatgta ggacttgcca aggaagaata
    56221 tagacctaag ctaatggact ggttatatcg tcaccatatt ccagatagta ttagcacttt
    56281 tggaccatat tgtactaaat atgcctttta tcaagcatat cctacaccaa atgaaggtga
    56341 gcgttttggt gcacgtaaga tgcaactaac agaacattat tggcttgtag atgaacatat
    56401 gcctgagatg gcaaatagaa ttatgacaga atatatgcct atggatgttc tacgttggca
    56461 agggtgtata ccagatgtag aaaataaaag ggttcatgaa aatgcagaaa gtggagatgc
    56521 aggacgtgca gtaggtggag ataatggatg tccaccattt atatttgcct ttgttccaat
    56581 aaactgggaa gaagacttta gaggaaaagg acgtactgta caagatggac caaactatcg
    56641 ttggcaattt atgattaagt atccagatgg tatctctaaa gaagaaggag aaaaatggtt
    56701 ctatgatgag gtagtgccat actttacaaa ctgttgctat gttaatcgtt ttgtcagtag
    56761 taaaataatg attaattatg gagcaactgc ttttgaccgt gtgtcagaac tatggtttga
    56821 aggggaagaa gaatggtata aagctgtggt tgaagagaca aagtcgttta ttaaaaaacc
    56881 agaatgggca caagaagagg agttcccata tttaaaacca caattcaata tcgcatcagt
    56941 attcttaggt gatatagcaa ctatggatgc atactcacag tatcgtggat atataccaat
    57001 gagataaaaa ttatagtttg tgataagtag agaaggcatt tactcttact ggtagaagaa
    57061 gacttattgt atccttaaaa gtaggtgaat atatagtttt atatttaaat ttatataaaa
    57121 ctaagactaa taaatatgaa acaatagaat tagaataatt tgataatatt tgttaaaaga
    57181 gcatttagaa tgtgtttaaa gattaagata ttccttttat ggtagcagat agttgaaata
    57241 ataaaactgt tatcatgaaa ggaatatttt tatgtatagg aaatttttaa aaataaaagt
    57301 taaatatgtt taagaacatc ttaaagtgaa agaatcattg ttagaaatat gtagacaata
    57361 tagtatttat aacgtatcat attaacaagt ttacacttgg attatgaaat aaaaagagta
    57421 attctaaagg ttgtaaatac tatgctgact ttgatacttc aaagtttagg tgattttgta
    57481 attgttaata gaaatacgtt taatgagcat aaagaattta tctaataata ggagaatata
    57541 atgtatacaa ttggacaagt atctaaattt ttaggaatat ctagagatac actaaaattt
    57601 tatgaagaca aaggattagt aaatcctaag aaaaatgatg aaaatggata tagaatatat
    57661 aatcaggttg atatctatga tattgcaaca ataaactttt atagagaaat tgatatagaa
    57721 attaaaaaga ttcaagaaat aagaaaaagt aaaagtataa acaatttaga actcttacta
    57781 gaagagaagg aacaaatcat actaaaagag atagaatata aaaagcttct cttaaaaaag
    57841 gttcagttag tcaaagaaga ttgtgacaaa atcaaaacat attttggaaa atacacaata
    57901 aaagaaatga agcctttgga agtaaaaggt aaaataaaac atgttactaa ctatgaggaa
    57961 tatgaagcct taaaagagaa tacaaacaac ttaaaaaaag cagttacatt aactgattta
    58021 agaagagtaa taaattttaa tgaagaaggt ataatagaag aaaaatttat aattgtaagg
    58081 gaagtagatg actacaacaa aatcatagaa ggtgaagttc tatctcatcc caattgtatt
    58141 tatactatta ttgaagatgg cagatggtca actggaggag aaaatattga taataaagtg
    58201 gaagacagat taagagaaat agcaatagaa aatgaatgtg aattacttgg aatagtctat
    58261 gttaatatat tgcttactac ttatgaagaa gggattgagc gtatattttt agagatttat
    58321 gcacctataa aataatataa aagcattgac ctgggactta ctcataggtt taaactcatg
    58381 atataagcag ttataactta tatagcaatt aaatattaat ttgaaatgag gattacttat
    58441 gagaacagag aaatcattaa aaaggcctat agccagtttt attattctaa caaatataat
    58501 ttttttacca ctttttttat tggtagtagt gactataatg ttaaaacttc caacagtgat
    58561 atctgacatt gcgttgtgta tatcagcgtg gtcatccact tttgcattta tgatattatt
    58621 taaaaaaatt tatccaggta agaaattttt agtacatgta aaagatagat ttagaaataa
    58681 acttaaattt tcaacagtat ctataataat tagtatacaa gtcctaatag ctgtagtagt
    58741 tatatttata ttagcatcta aaaatcatgg aataatgcct aactttacag tttcatcatg
    58801 gggagcattt atatatttat ttttaaaaaa tttatttgca ggatcattag gtgaggaatt
    58861 aggttggaga ggatttgttc aaaatcatct tcagaaaaga cattctcctt taaaagcatc
    58921 cataacagta ggtttttggt gggggatgtg gcatttacca atatggttta caacaggatt
    58981 caaaggactt gatttaataa aatatattat attttttatg attgcaatta tatcaatcag
    59041 tattataatg acaacttttt ataatctaaa taaaactttg ataataccta ttgcaataca
    59101 tcaactattt aattttttaa tagggataat taatgaagat ttaatagagc ttataaatta
    59161 ctatgcaatg ttatatttaa tatttgcaat tgcattaata gttataaatc caggaaatgt
    59221 attatataaa aatattagag agtaatatat atctttatgg taagatataa agatattatt
    59281 agaagaacat ccaaggagga ataaacatgg ttttactgat tgttgatgca caaaagttaa
    59341 taacaaacga gaggttatat aaatttaatg aatttgtagt aaatgtagaa aatttaattg
    59401 atacagctag aaaaaataat atagaagtaa tatatgtacg tcatgatgat ggagttgaaa
    59461 atgaattgac taaaggtaaa aatggctttg aaatatacga aaaatttaaa ccatgtaatg
    59521 gagaaaaaat atttgataaa aaagtaaata gtgcttttaa agaaactggt ctattagaat
    59581 acctaacaaa taatggtgaa aaagatatta ttattgctgg tctccaaaca gattattgta
    59641 ttgatgccac tataaaatgt ggatttgaac atggatttaa tataatagtt ccagcttatt
    59701 caaatacaac tgttgacaat ttttttatgt cagcagaaca atcatataaa tactacaatg
    59761 aatttatatg gaatgaaaga tatgcgaagt gtatttcatt agaggaaaca cttagaaaaa
    59821 tgaaataaaa atagagtatg aattgtatat gtacataagt tgatttcata aaaaaggatg
    59881 ttaaattcca agttaaaaat tagattaaat atattatatt atagataagg cacatcaatt
    59941 ttattttaaa ttaatgtgct ttttaattgt agtaatttat ttataatcaa tatgattttt
    60001 attatttaaa tcaatttttc ttgagtttct ttgtctaaag aaactttttt tatttataaa
    60061 atctacagtt atattatagt atctatttta gatgagttta atttttgtat caatctaaaa
    60121 caaaagttat acttaaatga aattgaaaac gataaaattc tcaaataaaa taagtattgt
    60181 ggaggtgatg aaagaatcca atgataatat gaagactaat cttaataaga aagtaggggt
    60241 taatggtagt ggacaaagaa attgtatcaa ttctccagat tttattaaat agcacattga
    60301 ttacaactaa tggattacaa gaggagtcta attcatcaaa gagacaaatt acttatagaa
    60361 taaataaaat caatgacatg cttaaagtaa aaaatgtacc acttatatgt ttaagagccg
    60421 acaaagatat tatcgttaga aaagaaacaa aaaaagcaat taaagaaatt ttagaaaaaa
    60481 actattctaa gaatacttat tattttagta agacagaaag acttttatat atgtatttaa
    60541 tgctttttat taacatggag gatatttcaa ttaatcattt tataaattct ataaaagtaa
    60601 gtaggtctac tgtaaatctt gatttcaagg atttgatacc agagcttgaa aagaagaaca
    60661 ttaaagttaa gaacaatcgt ataaatggat attacatagt tggcaatgag atggaaatta
    60721 ggagagtact tattaaaaac attatagaaa ctttgtctaa tgagaaaagt tcaagagtat
    60781 ttgatatttt tattaaagaa tataatttag atagttttga agaggctaaa aaaattatat
    60841 taaagctaat aaaaaaatat gaaattactt ttgttgaaga tagattaata gaatttatat
    60901 atatatttat ctttctaaaa gcaaggatgt attcaactag aatagttact caggaaaatg
    60961 tagatattcc taatatttca gttatgagtt caatgaagga gtataaattt gctaaggaat
    61021 tattagaagt atatgggagt gaagataaga taaagcctca caatataatg tatattagtg
    61081 cttggatttt aggtatatca gtaggaaatt atgaagaaga tacagaagat aaggatgtaa
    61141 tttcaaaaat tgtaaataaa atgatgacaa agtttggata tttaagcgga gtttactata
    61201 taagccaaga aaaaatattt aaacaattat attctcattt tagacctgcc tactatagac
    61261 ttttatttaa attacctatc tataatccga tttgtgaaaa agtaaaagaa gaatataaat
    61321 tagtttatag aattgtaagt aatgcaatga aagagttttg cggattattt ggagaagaaa
    61381 ttcaggaaga agagttagca tatcttacaa tgcactttgc aacacttttt tcaaataaaa
    61441 aagaatttga tggttttaga aaaagaatag cattaatagt ttgttcaaat ggcgttggta
    61501 gttcagctat tttacatgca gaacttacaa acttatttcc tgacttacac tttataccaa
    61561 tgtcggaatc agttggatta gaaaatataa gtgaacctgt agatattgta tttactacta
    61621 attataacgc agagggttta aaaacagatg taccagtaat taaagtaagc cctgttatga
    61681 ccacaaaaga aaaatataag attacacgtg aagtgtatat tcaattaggt gatatttttt
    61741 taagacaacc taaaatagat gaggttatgg atataataaa aaaatatgca aatgtaacct
    61801 ctgaaagtat tctttcaagt gagctattag cttattttac acagatagag aattttacat
    61861 ctaaggaagg agaggggcct atgctaagtg agattactaa tgaaacacta gtaaaaataa
    61921 aggtaaaagc taagaattgg gaggaagcta ttagacagtc agcttctgta ttagttgaaa
    61981 ataacaaagt aactgaaggg tatgtagatg ctatgattaa ctcagcaaaa gcatctggac
    62041 catacatagt aattacaaaa catgttgcac ttccacatgc tagaccagaa gctgggtcaa
    62101 aagagattgc aataggcatt gctactttag atagtcctat tgagtttggg aataacgaaa
    62161 acgaccctgt aaaatatgta ttttgtctaa gtgcaataga caataatagc catttacgtg
    62221 ctatgtctga gctagttgaa ctattggaag aagatgagtt ttttaaggtt ttagatagtg
    62281 ctaaagaagc taaagaaatt attgatttta taaaaacaca cgaattataa aggaggattt
    62341 aattatgtat aaaattttaa tcgcatgtag agcaggagtt ggctcaagtt taatgttaaa
    62401 aataaagact caacaaatta ttaaggaaaa taactttcca attgaagtag agcatggttc
    62461 attggattca ctaaatggat tcaatggaga tgctgtagaa acattaattg atgttgctga
    62521 agaattaaaa aataaaaatt tgaagtttga tataattggg attcacaata ttgttgaccg
    62581 taatgaaata aagacagcac tagaaaaatt ccttgcttca aaagagggat aaatttaaga
    62641 tgataaggag gaatatttat ggagtttatt attagtttgc tgagtacacc tgctgttttg
    62701 cttggcgtag ttgcatgtat aggattgatt cttcaaaaga aatcagctgt tgaagtattt
    62761 acaggctctt caaaaacact tatcggtttt ttgatatttg gtattggtgc ctctgctatg
    62821 acaggagcac ttcaaaattt caataaactg ttccaacatg gctttggaat tactggtgtt
    62881 atagcttcac cagaagcagc tacagcactt gcacaaggta catatggttt tgcagtttca
    62941 tgtacattaa ttcttggatt catattaaat ttagtatttg cacgtattac aaagtttaag
    63001 aatattttct ttacaacagg tcatagtttg ttcttttctt gtgtattagt tttaattatg
    63061 aaagcacatg gatttgataa tacaatcact attttaatag gtggaactat cttagggttt
    63121 atgtctgctg cactacctca attctgtcaa ccatttatgc gtgaattaac aggtggcgac
    63181 gaacaagcta ttggtcactt taacatgatt ggttatggtt tatcaggtta tattgggcgt
    63241 ttattttcaa aacataaaga tgatacaaca gagactatag aatttcctaa gtggttatca
    63301 atattcagag acttcttaat gggtctatcc atagttatgt taatcttatt ttatattgca
    63361 acattaaaag ctggtcaagc atttactcaa gaaattgctg gttcaacaca ttggttggtt
    63421 tttccgctta tagaggcatt tacatttgtg gcaggtatgt caatattgat gtcaggtgtt
    63481 cgtatgttct tagctgaaat aactgcagca tttgtaatca tttcagaaaa gtatattcca
    63541 ggttcacgtc ctgcgctaga tgttccaaca gtatttccat atgcaccaaa tgcagttatt
    63601 attgggttta tatcagcata tgctgctggt ctattagcaa ttgctattat ggcatcattt
    63661 cctagtatat ttcctgtagt aattattcca gctgctcaca tatgtttctt ctcaggaggt
    63721 acagctgcaa tatttggcaa tacttcagga ggatggagag gggctattgc tgggtcattt
    63781 gttgtggggt tattacttgc atttttacca gttatactat atccagttta tggcactctt
    63841 ggtatagaag gagcaacttt ccctaatatt gattacaatg tagttggttc gatattacat
    63901 aatattttac aatttataaa acctatttaa actaaattat ataaaagtat agtcatagtt
    63961 actatgattt caaggaggat acatcttatg gaaaatacat tatctgaatt tttagataat
    64021 atatctaatg aattgtcaga atttattgcc tcaatagatg agacatctat aaataaagca
    64081 tgcgaattaa ttcttgaagc tgaaaaaaat cacggacgtg tacatgtgac tggtattggc
    64141 aaaccaggac atgtatcggg atatatttca tcattgcttt catctactgg aacgtcagca
    64201 tatatattgc atggtacaga agcagtacat gggtcaagtg gtcaagtagt tgaaggagat
    64261 gttgttattg caatatcaaa tagtggtgaa actcaagagt taaaagcaac tcttaagact
    64321 ctaaaagtaa atggagcaaa gataattggt gtaagtggta atgagagttc atttttaaaa
    64381 aatatttctg atatattcct gtttgcaggt gttaaacagg aaggagattg tcttaataaa
    64441 gctcctcgtg cttcaatttt agctgaaaca ataatattac aatcattaag tgttgtatta
    64501 caatatgcta aaggtcttaa tacacagcag tatttgaagt ggcatccagc aggaagtctt
    64561 ggaaagtcta ttagagaggg aatataattt tatgaaatat aaagggatta tatgtgcaat
    64621 gataacacca tttgatgaaa atcaaaatat taattcacaa gcaacatgtc aattaattga
    64681 ttatttgatt gaaaagggaa tttatggatt atttattttg ggcactaatg gtgaatgtca
    64741 tgtattaaca gatgatgaaa aagttgaatt tgcaaagatt gttattaatc atacaaataa
    64801 tcgtgtacct gtttttgttg gaacaggggg taattcaact cgtgaagttg tagatttatc
    64861 taagagaatg gaagcgattg gagcaagtgc attatctatt attacaccat actttgttac
    64921 accaacacaa caggaattaa tcttacatta caaaactatt gctaattcag ttaatttacc
    64981 tattataatg tataatatgc caagtaagac tggaattaat attgaaccag agtcagtacg
    65041 tgaattatca aagataaaga atattgttgg aattaaggat agtagtggta aacttgacaa
    65101 catgaaagct tatattgaag caacgaagaa tgagaatttt agtgtgtttt caggctcaga
    65161 ttcacttatt ctggatagtc taaaagctgg tagtcaagga gctgttgcag caacaacaaa
    65221 cttcctcaca gagattggta tctctatcta tgaaaacttt atgaaagggg atttggaaga
    65281 agctcaaaaa gctcaaagta gtatagaaga acttcgacgt atactgaaac ttggaacaat
    65341 tccatctgtt atgaagaaaa ctattgtctt gaatggaatt aatgttggaa ctgcacgact
    65401 tcctgtaact gaaccaactg gggaagtact tgaagaaatt aaacgagtag ttgaaaatta
    65461 taggacaata cttaataaaa gtagtgagaa tagataatat aaatagggaa attacactgg
    65521 tatataatta atatatatat tataaaacat gactaaaatt aatgaataag taaagtggtt
    65581 tgatgagtat agaggtgata atatgaaaaa gatgaatata accaaaagaa tgtcagaatg
    65641 tggggctctt gcaattgtaa gagaagaaaa tcttaaccgt gcatgtgaaa ttgctgaagg
    65701 atgtataaaa ggtggtataa cagttataga aatgagttat accctaaata atgctggaga
    65761 aataattcaa gggcttaata aaaagtatgg tgaaacactt tgtgtgggag caggaacagt
    65821 tttggatagt gaaacagctc gtcatgctat attacatgga gctcaattta ttattgcacc
    65881 aaattacaat gaaggtgtag cgaagatttg caatagatat cagattcctt atgctccagg
    65941 atgtacatca ttgactgagg cagtagacgc actaagttta ggagcaactt ttataaaagc
    66001 tttcccaata tcagatttct atggacctaa actagtaaag gtatttaaga caccaatacc
    66061 tgatatgcca gttttagcaa gtggagggat taatattgaa aacctacata tatggttaga
    66121 aaatggagtt gatgtatgtg gatttggtgg tttattaact aaaggttctg ttgaagatat
    66181 tgctaaaaat gctaaaaaga ttcgtgaaat cattaaaaat ataagaaaaa tttaacatag
    66241 ggctgtatca tagtatctta taattactat gatacagcct ttttaatatt attcaaatta
    66301 aaaacagttt acatttctat tatattacat ataatttcag taattttatc ttaatttttc
    66361 tatctggaaa aaaatgattt aaaacttaag ttatgatata atttcattaa aagaaggcga
    66421 tttttagtaa atatgagaaa ataatgtgtt tttaggggaa aagttaaata cagatttaag
    66481 tgaacaagaa tcgataatta agatattaaa tggtgaatag ggcatcaaaa aaattactaa
    66541 attaaaagtt ttagagggag aatcttagca agtgtgtgtg aaaattcaat ttcaaacaaa
    66601 aaataattgt aaatgaaatc aatttataca cgttgaagat tcacttttat cattatatta
    66661 aaaagcagag tgaattaaaa gtgagtctaa agataggagg ataattaaaa caatgaaaga
    66721 agacattagg ttacatcgaa tgtgtaaaaa agaacaagca acaaagaatg aagttatatc
    66781 agaggatatt ctaaaatcta tgagtattgg ggtcagtaaa cattttttag atgattcatt
    66841 tacaataata tggggaaata catcattcta tcaattatta ggatatacag aagaagaatt
    66901 tttagttaaa ttttctagtt taaaagatta ttatgaacag aatctttatc attttgaatc
    66961 tatgaaaaat cattttttag aggcttatca tcatggcgaa aagagtatag aatatcaaat
    67021 ttgcatttca gcaaataatg gagaccctgt ttgggtaata atgactggta tattaattga
    67081 atcacaagat aatgagaaat caatggtcta tatattgtat tcaaaaatta atgagttaaa
    67141 aataaagcaa gaagaactat ctgatatgga aaaagagagt acagagaatt ttaaatggat
    67201 gatgtcagag tatgctggga atgtttatat tagcgatatg gatacatatg aattactata
    67261 cttaaataag cactcatgca atactttaca ggcatcagca aatgaactta ttggaagaaa
    67321 atgttatgag gtaattcaag gtagaacttc gccatgtccg ttttgtacaa attcgtattt
    67381 aaaaaaagat gacatatatg aatgggaatt ttataataaa aaactgaaac gaacatttat
    67441 aattaaagac cgtatgttga attggcaagg gcgaagagtt cgtattgaat tatcttatga
    67501 tatgtatagt gctgaatata aattagcaaa aaaagatcag gaaagagaat caattttgaa
    67561 aactattcca gcaggaatgg ttagattaga tgcaagagat tatcttacta tactttggtg
    67621 taatgatata tttttgaata tgattgaata tacaaaggaa cagtttgcag aggaacttaa
    67681 taatcaatgt ggatatttat tttctgaaga ttataaacgt agtaagaaac tggcacaaga
    67741 tttgaaggag tctggggata atgttgtatt tgaagcaaaa atatatacac gttcaaggga
    67801 agaacgtata tggactgtta cattgtgtta tatcagtggg gaagacagtt gggatggaat
    67861 accatctttt tatagtatag gtattgacat aacaaaggaa agaaaacaga ttgagaaatt
    67921 acagcatata tcagagaaag atgcattaac aggaatttat aatcgtgctg agactgaaag
    67981 gcagattaag aaatattttg aaaaaaattt aaatgttatg ggtgcactgt ttatgattga
    68041 tacagataat tttaaacaga ttaatgatac agaagggcat atgattggag atatagtact
    68101 tactgagatg gcatcaggta tgaaaaaaat aatgcgtgac agtgatgttg ttggaagaat
    68161 aggtggagat gagtttacaa tttttatgaa gaatatttcc tcagttaaag atgcagaaaa
    68221 gaaagcagaa gaactattgt atatgttccg tcatctattt cagaaagaaa aaagttttct
    68281 aaaagtaact tgtagcatag gaatagctat atatccaaag gatggtacta cattcaagga
    68341 aatatatgca agagctgata aagccctgta tcaagcaaaa aatatgggaa aaaataatta
    68401 tgtaatctac aaccaagatt attttaaaga attagaagaa tttgattgtt cttctcttgg
    68461 tacagttatt gattcagaaa aacaatacgc tgaatatcct gataatctta cacgttatat
    68521 attccgtatg ctttatcaga ctgatgatat agaccaggcg attaatatga tacttgaggt
    68581 tgttgggaaa cagtttgatg taagtcgtgt ttatattttt gaaaatacag aagatggtag
    68641 atatacttct aatacttatg aatggtgtaa tgaaggaatc tcttctcaaa tggcttattt
    68701 gcaaaacagc aattatcaag actatgagga ttatgaaaag ctttttgagg atgaattagt
    68761 tttttattgt cgagatatcc atacattaag tcctaaacta gaagaattgt tttctagtca
    68821 aggtatccac tctacacttc aatgtgcata taaagaagat agagttttca gtggatttgt
    68881 tggatttgat gaatgcacag gactacgtct atggtcacaa gaggaaataa gtacattatc
    68941 attgatttct caaattatat caatgttttt acaacgtaaa aaaaataata aacttaatca
    69001 acagattcaa caataccaga atattttaga taatattgac cagtgtattt gtgtgattaa
    69061 taaagaaaat aattttttat tatatgtaaa taaaaaattt aaaaatacat attctaaatt
    69121 ccaaattgga caaccttatt atcttgattc tatagatatt cataagatat ccattatatg
    69181 gaataaaaaa gaagcatacc tatgtactag cacgatttaa tggtaaagta aagtttatat
    69241 tggacttaaa ttaaatttcg ttttaaataa gatattattt tagttgctta ctgaaaagac
    69301 tagaatctat attaatgaat tatactaatt ttataccact attaaaatat tgtttgctaa
    69361 aaataaaaaa tagccttaaa acagttgatt ataaaaaatt aactgcttta aggctatttt
    69421 tatctttgtg acgaaataga cacttgccat ttagaatgtg acaatatagt agttgtttag
    69481 aaaaggcaca aagcattata ctttaactaa agtaaaatgt attttgcttt tagaataaaa
    69541 acaattagga gggagctata attgaaaaag ataatatatg tattatttac aattgaacta
    69601 ttaatagtat ctttattagg tctaaatata gttaaagata atgaaattaa taatatttta
    69661 tataacgaga caacaagtat atctgtaatg tttaaagatt ataaaaagtt aaataaaaat
    69721 tatagtaagt ggattaaaga tattgcagat gaaaataatg tgactatatc aaagtatgtt
    69781 tttaataata atgaaaaatt atctatatat acgactgata caagtttgaa taacaatata
    69841 aagttaaaat caggaaaatt tcctaatact aattcaaatg aatttattag taatataaac
    69901 actaataata gtaatcaagt aggtaaattc tcaactatga gtaaagacat atcaatttta
    69961 attagggatt ttggtaaaat aagtgaagtt ggagaatcag gaatcttata tatttcaact
    70021 caaaatgaag atacaataaa taaaatatta agagaactaa atgtagacag aagtattttt
    70081 gtagcaagat tacatgatag atatgttaac tcaaatttat acttaaatcc ttcaataatt
    70141 agagatttaa tactaattat cctttgtttc ttggctacaa taatacatta ttcaattagt
    70201 gtgagtagag aggcttctat tttaagatta aatggatact ctaagctaga tataattgct
    70261 agagttataa aaaatatact tagaattatg attttatcat ctgtcacagc attattaata
    70321 tatataatct atattttaaa attaaatata ggtgttagag catgcttata ctttgctatg
    70381 ttttcgatta tatgtatttt gataaatatt ttgatatcta tattaatagt tggttttaat
    70441 agtagaagtt ctaaatatgt gctatctcta aatggaaaaa aatcttatgg ttttgtaaat
    70501 ataatgcatt tcacattgaa aattgtattt gtatcatttc ttatattatc cataaataac
    70561 tgtattttaa attataatat gctacaaact cagttaggaa atctatctga gtgggataaa
    70621 gcaaaaaatg tttataatct tactcttaaa gatacaggcg aacaatcttt aggaaaggaa
    70681 gaagtaatta aaaatgccaa gattaaaggt ttctataaaa atttggtaga agaaaaagat
    70741 gctttcttaa tagattcaac taattttgaa aaacttgaag atggaagtta catgtatgaa
    70801 ataaattcaa agggtaaaaa cccagaagta agtcaatatg gtaaaaatat aaaaataaat
    70861 aaaaactatc ttaaagttaa ccctatttac tcaaatggca aagaaatatt taatctaatc
    70921 gattatgacg ataagactat aaatttatta gttcctaaaa agttacaagt acatgaaaat
    70981 gagattaaaa aagagtttag agaattattt tattttgaaa aaatagaagt agaaaatatg
    71041 tacaataaag agttaaatag agcgttaaac aaaacaaaga agtcagattt aaatgttaat
    71101 ataatatatg tagataataa tcaaagctat tttacttata actcccttgt tatggatgac
    71161 aataggaatc tcattgatga cccaatagca atagttgaca tagataatat tgatgattct
    71221 ttttatcttt cttatatatc tagatgtgta tattttaatt ctaagaaatt agatgccctt
    71281 gcagatataa gtagcattat tgaagctcaa ggtgtagaag cgcatatcca aagtttacat
    71341 gctgtttata atgagtatgg tttagaaata aataaactag aaaaatattt gaatagtgaa
    71401 atttttacta taataataat agcaatatca aacttaatga ttacctacaa tatagtagct
    71461 agctactatg aaagaaataa atataagtta tatattaaaa aaatatttgg ttattcaact
    71521 acagctagaa gtatgttgtt aatagtctca ttaatattaa caaacattat tccaataggc
    71581 attatatcaa caagagtaga tttaagtaat aatattatct tatttggact tctaatatta
    71641 gttatagaag taattgtatc tattgtatta gacaagatta tctcaaatag ttcatttaat
    71701 aaaattataa aaggagaaca ttaaaaagat gatttcatta ataaatatat ctaaaaaatt
    71761 taaagataaa tctataatag acaaatttaa cctagaaatt tctaagggcg agtttgtagc
    71821 gataacagga aatagtggtt caggtaagac aactttactt aatataatgg gattattaga
    71881 aaaacctaat tctggtgata ttataataaa taatataaaa aatccaaata gtaaacagat
    71941 aagaatttta caaagggatt tttatggcta tctatttcaa aattatgctt taattgagaa
    72001 tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat caaaaaaata tcaaccataa
    72061 taaaaaaata aattacgcac ttgagtctgt aggtcttaga aaatacaata aaaaaaagat
    72121 atacgaattg agtggaggag aacaacaaag ggtagcatta gcaagagtaa tattaaaaga
    72181 atgtgaagta atatttgcag atgaacctac aggtaattta gataaaaaaa atagagacat
    72241 agtatttgat atacttaaaa gtctaaataa gaatggtaaa actatcgtat ttgtaactca
    72301 tgatttagag ttatcggagc aggcagatag agttataaaa ttgtaataat atcaactata
    72361 ataaatataa gaaagagatt acagctaaat caagtttaat aataatacga atagaattta
    72421 aacattaagt aaattagtag tttgtaaata taagtataat atgatatcat aaaggctcaa
    72481 aagaaatttt atataataag aaatctaaaa ataacaaatg gaggacatat aaagatggaa
    72541 aatttaccaa attgcccgaa atgtaattca gaatatactt atgaagatgg aactctttta
    72601 gtttgtccag aatgctctca tgagtgggca ttaggattgg gagctgaaga agattcaaat
    72661 attatcaaag atgtaaatgg aaatgtatta aatgatggag attctgttac agtaattaga
    72721 gaccttaaag taaaaggaag ttcatcatct ataaagattg gaacaaaagt gaaaaatatc
    72781 cgtttaatcc atgactcatc agatggacat gatatagaat gtaagataga tggttttgga
    72841 gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat attataaata agctgtagtg
    72901 tttattgttt ttcagtacag tataaaacca taagaatcta ttaagtaata aaagtttgtt
    72961 tatttatctt tatattttct tatggtttta ttaaatataa attttattga acaccaaaat
    73021 attctctcct ctacaaataa gccaatctta tttaaattaa tatactttgt actatttata
    73081 ctgaaattgt aaatttaaat gctatgttat ttaggtttgt atagttaatt tatatatttc
    73141 tgattttata aattgtattt attattatat cttgattcta aaatcattaa aaatttcaac
    73201 ttctataaag atttacaatt tatcaaatac attatgagta tataaacaat ttattattac
    73261 agtgctacat ttaaaataag tcttagtata aaggtattgt tacaagtata aagaaactta
    73321 tgtatgctat tggaataaat aataccttaa attataataa agaataagaa ctgttaaata
    73381 agggacttga ttatcattta gttatagata atataatatt aaaaataaag tttaagagga
    73441 gaaaaaatat gatagatata cataaaaata tatatgataa taagttattt gaggagctaa
    73501 agattgactg taagaagtgc tttggacttt gctgtgtagc tctttatttt tctgcttcag
    73561 atggatttcc tatcgataag gaatcaggaa aaccctgtat taatttacaa ccagatttta
    73621 aatgttctgt acataatagt cttatgaagc gaggttttaa agggtgtact gcatatgatt
    73681 gttttggttc aggtcaaaaa gtagcacaag taacatataa ggggatagac tggatgcaat
    73741 catcagaact aacaaatcaa atgtctgaag tgtttttaat tatgagacaa ttacatgaaa
    73801 tgttatggta tcttaaagaa gcatctgtat taaatatatc agatacaatc aaatcaaaaa
    73861 ttgatttaat aattgaagaa actgaaaaga taactaatat gagcccagaa cagataatta
    73921 atttggatat catatcacat agaataaagg ttaatttatt attatcacaa gcgagtgagt
    73981 ctgtgatggg aaaggttaaa tcatttatca agacttctac tcttaaaaac atgaaaaaat
    74041 taagtaaaaa tatagattta ataggagcag accttagaaa aataaatcta ataggagcag
    74101 atttaagagg tagatttcta atagcagcaa atttaagaaa tacagatttg agtggggcaa
    74161 atttaatagg agcagactta agagattgtg atattagagg tgctaatctt gaaaatagta
    74221 tatttttaac acaattacaa gtaaatactg caaaaggaga ttctagtacg aagttaccag
    74281 cttcactaat acgccctaaa tactgggaaa aataattaaa ttaatacata attttacaaa
    74341 acatgaactt ttttgacttt aattcatatt atacattaga gattctttac aagaatttct
    74401 taatataata tagggagaga tataaaaatg agttatcaaa attataaatg tgaaagagat
    74461 tatgtgtatg atgattataa atacaataag tgcaacaagt gctataatga ttatgaggaa
    74521 atgacacatg ttcatgaata ttcagaaagt gttaagttag cagaagaatg tgaggataga
    74581 cataaccacc gtgcagcagg ggttactggt gaagctatac caataaatgg aggtacaaat
    74641 catgttcata aaataaatga taatgtagat ttccttgacc atttccataa gatatgtgtt
    74701 acaacagggc cagcaattag aatacctgga actgataaac acattcactt aatatgtgga
    74761 gaaactactg ttaatgatgg tcattgtcat aaattccttt ttactactca aatagaagca
    74821 cctttagtat aagctgatgt gaattattgt agtgaatttg ctagattata gtataataat
    74881 atataataga taatatttaa acttaatgag gactatgata tttaaccttc gtagtccttt
    74941 ctaagttttg tataaaaaag tgttatgcaa ttaaaagtga tatagggagg aaatttttat
    75001 gaaatctgga ataattcttg aaatgaaaca aataaattca acagaatatg acgaaatgtt
    75061 gcaagtctgg gaatcatcag taagagcaac gcatgatttt ttaacagaaa aagatattga
    75121 atctttgata ccattagttg agattggact taaagaagta gaaaatatag tttgtataaa
    75181 agataatgat ataataaagg gctttatagg aatagataaa gataaaattg agatgctctt
    75241 tatagaagat aaatatagag gaaatggtat tggtaaaaaa ttaataaaat atgctataga
    75301 taaatacaat gtaaaatatg ttgatgtaaa tgagcagaat aaaaaagctg taggatttta
    75361 tattcactta ggatttaaag tgtttgatag gtctgaaata gatggacaag gtaacccatt
    75421 tccaatacta catatgaaat taatttaaag ttatacttct catttattcg caaaaatatt
    75481 tacttaaatt tttatacaaa taaaagtcgt gacagtctta ggacacgact tttttactac
    75541 actattaaat tgaaatttat ctgttctata ttatgattat tagatgtcgt ttttttattt
    75601 taactatgta cttaaatacc aagtataccc atacctatca agtacttcag cagcgccatc
    75661 attttgatgt ggtggtgggt gtggtaaaaa ttcttgtaca agtgtaccac cttcggcaag
    75721 ttttacagat atttcttcta tcgcttctcg agatggtaga acaacacaaa tcgccatgcc
    75781 agaaattaca acaggcttgt ctgtatccgc acccattata caaccttcgt attttgtgaa
    75841 agtaacactt gcgtgcataa taggattgtt tggattgctg tctaatcgcg caaatatagt
    75901 ttttcctcct ccgaaagcct cagcataaaa attaaatgct ttttcacaag taccattaaa
    75961 tattaaatat ggagttagca tgaccaatat atcctccttg atatagaata atttatagtt
    76021 gcacctgata atctaaatta tgatttagta aattgtatca tagggaaacc ttgaagtata
    76081 taatttttta ttattatatt atacaaattt attaatttaa aaaaatgtta tatgtacgga
    76141 aggtttacag gataaaatat aataaaaaga ttaaggtgac attataaaaa atgtatattg
    76201 acattgacgc tacgtaattg tatatacttt gtcttaaagg agatggtaaa tatgaatatt
    76261 atagaaattc gctcagataa aatatacaag aagataatgg atgcaccaat aaacaaaaaa
    76321 gaagatatat acagatatga attgatgaag ccttttgaat ttaagtggaa gtgtatgaat
    76381 gttccaatag ttgctagaca gaaaggtgga tatgatgtaa ttatagcaag tgaaatgtta
    76441 ggggttttat cgcctaagga tattgatgaa aaacaaaaaa agaatataaa tgtgttatct
    76501 gctgataaaa tttgggccac ttgtaaagaa accatagaaa actctataaa tgcttttata
    76561 aaagaagggt atgatttaaa tattaaggac tataaatatt caatattatt ggcgaatcca
    76621 aatagtcctt atacaatatt aagtgatgga tactggggtg atggtgggat tcctggatat
    76681 atatttctat cattggttcc taatgaatat actatcaata gattaccagt attaatagca
    76741 catgaatgta atcacaatat tagatttcag tttatagagt ggaataataa tataacatta
    76801 gaagaaatga tgataaatga aggtcttgca gaaaattttg caacatggat gtttggagag
    76861 gaaatgttag gaccttgggt cagtagaaca gatatcgaaa cattaaatac ttatataaag
    76921 ccaataataa aaagtgcttt aaaagaaact ggatttcaaa atataacatc ttatctttat
    76981 ggtgatgata tagctaaaat gcaaggatat tttccagtag ggttgcctta ttgtgcagga
    77041 tatgcttgtg gatattatat gattaagtat tatttagaaa agacaaataa atcaataatc
    77101 gaagcgactt tattgcctta tagtgagata atcgaagcag taaaagagtt ttgggaataa
    77161 attttataaa ttgcataatt tgaaataaat tatgcaatta ttttattttt aagaaaaaat
    77221 aaattgacaa taatttataa gaaatataca ataatatata taaagaaata tttcaatatt
    77281 taaaagtttt aagatttcat atctagagta cctaacatta catgttagtt tcttgttact
    77341 tatctaatta ctttatattt cgtagttata tataaaatta ctataaaaaa tgaaatatat
    77401 taaacaatca actaaaaaat gaaatattaa taaaaaaaaa ttcaaattat aaatattaaa
    77461 tgaatataat taaaacaaat atataaattt aagtataaca ctcacatttt aatgtattgt
    77521 gaaatttttg ttgaacttta tgtaccataa cttataaaat ttatgtcatg ttatatttga
    77581 ataagtaata aaattgtaga tgtaaattaa tctacaaaat ttatatgaaa tggagttgat
    77641 gctatgacag aattaatgat tataagtggg ttagtactat tgatttgtat aacttctagc
    77701 aaggtattgt ataaatttgg agtaccaata ttattgattt ttatcttact agggatgtta
    77761 tttgggtctg atgggatggt aggaatctat tttgataact atgaactgac aaaacaatta
    77821 tgttctattg gactaatatt tataatgttt tttggagggt ttggaacaaa ctggaatatg
    77881 gctaaacctg ttgctatacc ttctatatta atgtcttcac tgggtgtaat aattacggct
    77941 ggggttacag gtatgttttg ccacatggta ataggaacta gtttacttga aggtttatta
    78001 attggggcaa ttgcagcttc aacagatgct gcatctgtgt ttgctgtgtt gcgttctcaa
    78061 aaactaaact taaagggttc acttgcatca ttattagaag ttgaaagtgg aagtaatgac
    78121 ccaatagcgt atatgttgac actaattctt ttgacattga tgaataattc tggggtagaa
    78181 cttattgcac caatggtaat aaaacaaata gcttttgggt tagctatagg atttatacta
    78241 gcaaaattat ctgtttatat tttaagacat tcaaacttcg agatagaagg attttatact
    78301 atatttataa cagctattgc aatactttct tatgcattta gcgagtatct tggaggaaat
    78361 ggatatttaa gtgtttacat ttcaggtatt attataggaa atagtaggat acctcttaaa
    78421 aaaagtttgg ttcatttctt tgatggaatt tcatggatta tgcaaataat gttattcttt
    78481 atgttaggat tgttatcttt cccttctaag ttaccaaatg tatttggtat agcaatggcc
    78541 atatctatat tcatgatagt tattgcaaga ccattagcaa catttatagt tctagcaaaa
    78601 tttaaattct caaataaaga aaaattattc atatcgtggg taggtcttag aggtgcggct
    78661 tcagtagttt ttgcaattta tgctgttact caaggagtag ccatagagaa tgacatattc
    78721 catataatat tttttatagc gttgctttca gtaggaatac aaggttcatt gataccaact
    78781 gtagccagaa agttggattt agttgatgat aatacacctg tattaaaaac atttaatgat
    78841 tatgatggag aaattagctc taaacttatc gaagtaaatg ttgatgaggg taataaatgg
    78901 gctaataaga gtataataga ttctaatata ccagacgata tattaatagt tatgataaaa
    78961 agaaaggaac aggttttaat acctaaaggt tcaaccatta taaaaacagg ggatattttg
    79021 gtattgagtg gagatagaat tgaagaatta atacaattat aatttatttt gtattgactt
    79081 taacctaagg ggaaagttta aacttaaatt aatcaaatct aatgcgcagg tgatgagatg
    79141 ttttctatag gaatgttttc taaaataaat aaaataacta caaagacatt aaggtattat
    79201 gaagatatag ggttgttaaa accagaatat gtagatgaat ttacaaaata taggtattat
    79261 acaacagagc aattacctaa attacatcag ataataactt taaaacaaat gggtttatca
    79321 ttagctgaga taaaaaaagc tgtagaaaat cctgaagaag ttgaaaatat tttaatgaat
    79381 aaagaaaaag aaatgttgga tgtaattaaa agtgaagaat ataaaataat taaactacgt
    79441 aattacctta tgaatttgaa aggagaagtc aatatgaatg atgtaattat taagtcttta
    79501 ccaaaagtta aagttgcttc tatgagaaaa gtgttaaagg ggtatgatga actttttaat
    79561 ctatttccaa atgtaatggc tgaagaaatg atgaaaacag ggtgtacatg tgcagttcct
    79621 gattattgtt ttaatatata tcatgatggt gaatatagag agactgatat ggatgttgaa
    79681 atgtgtcaag ctataaatga tttaaaggaa gatacggata tattaaaatt taaagaatta
    79741 gatgcagttg aaaatgcagt ttgtatatta cacaatggtc catattcaac attaggaaaa
    79801 acttacgcag atgcatttaa atggatagag gacaataaat ataaagtggt tggaaatcct
    79861 agagaatctt atatagatgg tatatggaat aaagagagtg aagaagactg gttgacagaa
    79921 gttcagatac cagtgaaacg catgttataa tatgtctaca aagtataaat aagaataaaa
    79981 aaggtggtgt ctcaaagtaa acattttagt ttatgagaca ctctttttca tatgaaatca
    80041 agtatatttt tataattacg attatgaaaa aaggcttatc tatattttta gacagcctct
    80101 tattttttta ttataatagt tttctaaaga tgctctattt ataaattttt attaaagact
    80161 ttataaataa ttgtattta
//
LOCUS       DBGJFU010000003        70949 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.003, whole genome shotgun sequence.
ACCESSION   DBGJFU010000003 DBGJFU010000000
VERSION     DBGJFU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 70949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.003"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(157..450)
                     /locus_tag="ACOIAS_000190"
     CDS             complement(157..450)
                     /locus_tag="ACOIAS_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HGZ2699921.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(456..671)
                     /locus_tag="ACOIAS_000191"
     CDS             complement(456..671)
                     /locus_tag="ACOIAS_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2699922.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAMQQFIVENEILISEEV"
     gene            1120..1518
                     /gene="tnpA"
                     /locus_tag="ACOIAS_000192"
     CDS             1120..1518
                     /gene="tnpA"
                     /locus_tag="ACOIAS_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HGZ2699923.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            1515..2678
                     /locus_tag="ACOIAS_000193"
     CDS             1515..2678
                     /locus_tag="ACOIAS_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGZ2699924.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(3062..3574)
                     /locus_tag="ACOIAS_000194"
     CDS             complement(3062..3574)
                     /locus_tag="ACOIAS_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2699925.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            4024..4500
                     /gene="purE"
                     /locus_tag="ACOIAS_000195"
     CDS             4024..4500
                     /gene="purE"
                     /locus_tag="ACOIAS_000195"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGZ2699926.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            4506..5204
                     /gene="purC"
                     /locus_tag="ACOIAS_000196"
     CDS             4506..5204
                     /gene="purC"
                     /locus_tag="ACOIAS_000196"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGZ2699927.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            5216..6583
                     /gene="purF"
                     /locus_tag="ACOIAS_000197"
     CDS             5216..6583
                     /gene="purF"
                     /locus_tag="ACOIAS_000197"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGZ2699928.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            6577..7653
                     /gene="purM"
                     /locus_tag="ACOIAS_000198"
     CDS             6577..7653
                     /gene="purM"
                     /locus_tag="ACOIAS_000198"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGZ2699929.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            7647..8240
                     /gene="purN"
                     /locus_tag="ACOIAS_000199"
     CDS             7647..8240
                     /gene="purN"
                     /locus_tag="ACOIAS_000199"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGZ2699930.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            8233..9765
                     /gene="purH"
                     /locus_tag="ACOIAS_000200"
     CDS             8233..9765
                     /gene="purH"
                     /locus_tag="ACOIAS_000200"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGZ2699931.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAVVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            9776..11026
                     /gene="purD"
                     /locus_tag="ACOIAS_000201"
     CDS             9776..11026
                     /gene="purD"
                     /locus_tag="ACOIAS_000201"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGZ2699932.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTRVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            11051..14857
                     /locus_tag="ACOIAS_000202"
     CDS             11051..14857
                     /locus_tag="ACOIAS_000202"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGZ2699933.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDNKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVIGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            15063..15935
                     /gene="rfbD"
                     /locus_tag="ACOIAS_000203"
     CDS             15063..15935
                     /gene="rfbD"
                     /locus_tag="ACOIAS_000203"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HGZ2699934.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            16044..16913
                     /gene="rfbA"
                     /locus_tag="ACOIAS_000204"
     CDS             16044..16913
                     /gene="rfbA"
                     /locus_tag="ACOIAS_000204"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGZ2699935.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            16962..17519
                     /gene="rfbC"
                     /locus_tag="ACOIAS_000205"
     CDS             16962..17519
                     /gene="rfbC"
                     /locus_tag="ACOIAS_000205"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HGZ2699936.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            17557..18540
                     /gene="rfbB"
                     /locus_tag="ACOIAS_000206"
     CDS             17557..18540
                     /gene="rfbB"
                     /locus_tag="ACOIAS_000206"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGZ2699937.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            18570..19238
                     /locus_tag="ACOIAS_000207"
     CDS             18570..19238
                     /locus_tag="ACOIAS_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGZ2699938.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            19247..19675
                     /locus_tag="ACOIAS_000208"
     CDS             19247..19675
                     /locus_tag="ACOIAS_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HGZ2699939.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            19699..20013
                     /locus_tag="ACOIAS_000209"
     CDS             19699..20013
                     /locus_tag="ACOIAS_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGZ2699940.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            20165..20440
                     /gene="flgM"
                     /locus_tag="ACOIAS_000210"
     CDS             20165..20440
                     /gene="flgM"
                     /locus_tag="ACOIAS_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGZ2699941.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            20445..20861
                     /locus_tag="ACOIAS_000211"
     CDS             20445..20861
                     /locus_tag="ACOIAS_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HGZ2699942.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            20879..22189
                     /gene="flgK"
                     /locus_tag="ACOIAS_000212"
     CDS             20879..22189
                     /gene="flgK"
                     /locus_tag="ACOIAS_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGZ2699943.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            22208..23140
                     /gene="flgL"
                     /locus_tag="ACOIAS_000213"
     CDS             22208..23140
                     /gene="flgL"
                     /locus_tag="ACOIAS_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGZ2699944.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            23156..23548
                     /gene="fliW"
                     /locus_tag="ACOIAS_000214"
     CDS             23156..23548
                     /gene="fliW"
                     /locus_tag="ACOIAS_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HGZ2699945.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            23542..23754
                     /gene="csrA"
                     /locus_tag="ACOIAS_000215"
     CDS             23542..23754
                     /gene="csrA"
                     /locus_tag="ACOIAS_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGZ2699946.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            23776..24171
                     /gene="fliS"
                     /locus_tag="ACOIAS_000216"
     CDS             23776..24171
                     /gene="fliS"
                     /locus_tag="ACOIAS_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGZ2699947.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            24171..24527
                     /gene="fliS"
                     /locus_tag="ACOIAS_000217"
     CDS             24171..24527
                     /gene="fliS"
                     /locus_tag="ACOIAS_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGZ2699948.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            24548..26071
                     /gene="fliD"
                     /locus_tag="ACOIAS_000218"
     CDS             24548..26071
                     /gene="fliD"
                     /locus_tag="ACOIAS_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGZ2699949.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            26074..26409
                     /locus_tag="ACOIAS_000219"
     CDS             26074..26409
                     /locus_tag="ACOIAS_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HGZ2699950.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            26511..27383
                     /locus_tag="ACOIAS_000220"
     CDS             26511..27383
                     /locus_tag="ACOIAS_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HGZ2699951.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            27476..29617
                     /locus_tag="ACOIAS_000221"
     CDS             27476..29617
                     /locus_tag="ACOIAS_000221"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2699952.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            29641..31518
                     /locus_tag="ACOIAS_000222"
     CDS             29641..31518
                     /locus_tag="ACOIAS_000222"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2699953.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            31511..33625
                     /locus_tag="ACOIAS_000223"
     CDS             31511..33625
                     /locus_tag="ACOIAS_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2699954.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            33634..34629
                     /locus_tag="ACOIAS_000224"
     CDS             33634..34629
                     /locus_tag="ACOIAS_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HGZ2699955.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            34721..35953
                     /locus_tag="ACOIAS_000225"
     CDS             34721..35953
                     /locus_tag="ACOIAS_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGZ2699956.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            35973..36917
                     /locus_tag="ACOIAS_000226"
     CDS             35973..36917
                     /locus_tag="ACOIAS_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HGZ2699957.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            37011..37418
                     /locus_tag="ACOIAS_000227"
     CDS             37011..37418
                     /locus_tag="ACOIAS_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HGZ2699958.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            37496..38596
                     /locus_tag="ACOIAS_000228"
     CDS             37496..38596
                     /locus_tag="ACOIAS_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGZ2699959.1"
                     /translation="MNLLLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHF
                     EEEFANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGAT
                     PVFVEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDC
                     AQSHGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGS
                     EKRYYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKI
                     ENNCTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNI
                     AENYSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      38905..38995
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            39403..39720
                     /gene="flgB"
                     /locus_tag="ACOIAS_000229"
     CDS             39403..39720
                     /gene="flgB"
                     /locus_tag="ACOIAS_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGZ2699960.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            39728..40132
                     /gene="flgC"
                     /locus_tag="ACOIAS_000230"
     CDS             39728..40132
                     /gene="flgC"
                     /locus_tag="ACOIAS_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGZ2699961.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            40142..40459
                     /gene="fliE"
                     /locus_tag="ACOIAS_000231"
     CDS             40142..40459
                     /gene="fliE"
                     /locus_tag="ACOIAS_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGZ2699962.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            40484..42034
                     /gene="fliF"
                     /locus_tag="ACOIAS_000232"
     CDS             40484..42034
                     /gene="fliF"
                     /locus_tag="ACOIAS_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGZ2699963.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            42047..43117
                     /gene="fliG"
                     /locus_tag="ACOIAS_000233"
     CDS             42047..43117
                     /gene="fliG"
                     /locus_tag="ACOIAS_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGZ2699964.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            43101..43841
                     /locus_tag="ACOIAS_000234"
     CDS             43101..43841
                     /locus_tag="ACOIAS_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HGZ2699965.1"
                     /translation="MTLLNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFR
                     DEVIKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            43845..45164
                     /gene="fliI"
                     /locus_tag="ACOIAS_000235"
     CDS             43845..45164
                     /gene="fliI"
                     /locus_tag="ACOIAS_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGZ2699966.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            45195..45641
                     /gene="fliJ"
                     /locus_tag="ACOIAS_000236"
     CDS             45195..45641
                     /gene="fliJ"
                     /locus_tag="ACOIAS_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGZ2699967.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            45670..46875
                     /locus_tag="ACOIAS_000237"
     CDS             45670..46875
                     /locus_tag="ACOIAS_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGZ2699968.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            46893..47501
                     /locus_tag="ACOIAS_000238"
     CDS             46893..47501
                     /locus_tag="ACOIAS_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HGZ2699969.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            47528..48511
                     /locus_tag="ACOIAS_000239"
     CDS             47528..48511
                     /locus_tag="ACOIAS_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HGZ2699970.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            48524..48727
                     /locus_tag="ACOIAS_000240"
     CDS             48524..48727
                     /locus_tag="ACOIAS_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HGZ2699971.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            48727..49545
                     /locus_tag="ACOIAS_000241"
     CDS             48727..49545
                     /locus_tag="ACOIAS_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HGZ2699972.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            49535..50230
                     /locus_tag="ACOIAS_000242"
     CDS             49535..50230
                     /locus_tag="ACOIAS_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGZ2699973.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            50242..50685
                     /gene="fliL"
                     /locus_tag="ACOIAS_000243"
     CDS             50242..50685
                     /gene="fliL"
                     /locus_tag="ACOIAS_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGZ2699974.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIADLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            50700..51074
                     /locus_tag="ACOIAS_000244"
     CDS             50700..51074
                     /locus_tag="ACOIAS_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HGZ2699975.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            51075..51740
                     /gene="fliP"
                     /locus_tag="ACOIAS_000245"
     CDS             51075..51740
                     /gene="fliP"
                     /locus_tag="ACOIAS_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGZ2699976.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            51753..52022
                     /gene="fliQ"
                     /locus_tag="ACOIAS_000246"
     CDS             51753..52022
                     /gene="fliQ"
                     /locus_tag="ACOIAS_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGZ2699977.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            52034..53848
                     /locus_tag="ACOIAS_000247"
     CDS             52034..53848
                     /locus_tag="ACOIAS_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HGZ2699978.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNNIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            53857..55932
                     /gene="flhA"
                     /locus_tag="ACOIAS_000248"
     CDS             53857..55932
                     /gene="flhA"
                     /locus_tag="ACOIAS_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGZ2699979.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGDLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            55907..56923
                     /gene="flhF"
                     /locus_tag="ACOIAS_000249"
     CDS             55907..56923
                     /gene="flhF"
                     /locus_tag="ACOIAS_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HGZ2699980.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            56920..57798
                     /locus_tag="ACOIAS_000250"
     CDS             56920..57798
                     /locus_tag="ACOIAS_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HGZ2699981.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            57812..58513
                     /locus_tag="ACOIAS_000251"
     CDS             57812..58513
                     /locus_tag="ACOIAS_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2699982.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            58529..58951
                     /locus_tag="ACOIAS_000252"
     CDS             58529..58951
                     /locus_tag="ACOIAS_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HGZ2699983.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            58936..59121
                     /locus_tag="ACOIAS_000253"
     CDS             58936..59121
                     /locus_tag="ACOIAS_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HGZ2699984.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            59162..59935
                     /locus_tag="ACOIAS_000254"
     CDS             59162..59935
                     /locus_tag="ACOIAS_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGZ2699985.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            59956..60717
                     /locus_tag="ACOIAS_000255"
     CDS             59956..60717
                     /locus_tag="ACOIAS_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HGZ2699986.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            60740..61621
                     /locus_tag="ACOIAS_000256"
     CDS             60740..61621
                     /locus_tag="ACOIAS_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGZ2699987.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            61624..61998
                     /locus_tag="ACOIAS_000257"
     CDS             61624..61998
                     /locus_tag="ACOIAS_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGZ2699988.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            62030..63433
                     /locus_tag="ACOIAS_000258"
     CDS             62030..63433
                     /locus_tag="ACOIAS_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGZ2699989.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            63549..65486
                     /gene="htpG"
                     /locus_tag="ACOIAS_000259"
     CDS             63549..65486
                     /gene="htpG"
                     /locus_tag="ACOIAS_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGZ2699990.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            65873..67003
                     /locus_tag="ACOIAS_000260"
     CDS             65873..67003
                     /locus_tag="ACOIAS_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGZ2699991.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            67230..68252
                     /gene="splB"
                     /locus_tag="ACOIAS_000261"
     CDS             67230..68252
                     /gene="splB"
                     /locus_tag="ACOIAS_000261"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HGZ2699992.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            68434..69345
                     /locus_tag="ACOIAS_000262"
     CDS             68434..69345
                     /locus_tag="ACOIAS_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2699993.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            69360..69776
                     /locus_tag="ACOIAS_000263"
     CDS             69360..69776
                     /locus_tag="ACOIAS_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HGZ2699994.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(69876..70268)
                     /locus_tag="ACOIAS_000264"
     CDS             complement(69876..70268)
                     /locus_tag="ACOIAS_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2699995.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            70409..70804
                     /locus_tag="ACOIAS_000265"
     CDS             70409..70804
                     /locus_tag="ACOIAS_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HGZ2699996.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        29000 a         7352 c        12903 g        21694 t
ORIGIN      
        1 ataataacta tataaaatta tatgtttaga ttgtgagcaa tctattttat gttaaaaata
       61 gattatttag aagaagggga aatcttaaaa aaatttgaat atcccctttt tattatttaa
      121 ttaaaatgaa atagttagaa atatatttta tttagtttag tcaataacat tttgggattc
      181 tttataacta catgttaact gttgaatagg tttatcatat ggtttaaccc atcctttatt
      241 aatacttaat tctgtaagtg ctgtgtgacc ttctaccatt tgagttaatg aagctgaata
      301 tatagctctt aattcaggtg tactacttgt caatgcagaa ttaagataca tattggcaga
      361 tgctttagca gatgaaagca tacttaacat tattacttta tcgtctatat aagtattgtt
      421 cttaattaga ttttctatca tatttctcat gttatctata cctcctcaga aattagtatt
      481 tcattttcaa ctataaattg ttgcatagct ttaattcttc cttctgttgc taatatacta
      541 gattctgatt gtctttttag gtcttcatct gatattaaca tatttattgc tctttgcata
      601 agtaatccgt cagactccat ttttataact gctgctaaag atattttttc agtttctgaa
      661 agtattctca tttaatcatc tcctatattg taatttatgc tttaatagta tgtctttctt
      721 ttcaaggaaa taaccattac acttttttac agtattttat attctcttta tatcaaaatt
      781 tagtcgacct aatatattaa taataatttt gtaggatatt tatcattaaa tatctataaa
      841 ataaattatt tgaatattca taaagcaaaa tttatttgta tgtacttctt caagagtcaa
      901 tatgagtttt ttcaaaaaat taatttttat gataatcatt aagggctata tttttcttaa
      961 agttgtgatt atcattatta atttacaata aaattgccga aaagccctct cctttaggtg
     1021 tagggatgaa aggtaaggtc ttaatataaa aaaagttaga ataatgcttg taatctaata
     1081 ccgctactga taccctatat ttaggaggtg ttagttttta tgtataaatc taacaaaaat
     1141 atagtttatg attgcaaata tcatattgta ttttgcccta agtatcgtaa acctgtttta
     1201 gttggcgaag ttgaaaagat gttaaaagaa atactaccat ataaagcaga tgaacttgga
     1261 gcagaaataa tagaaatgga aactgataaa gaacatgtgc acttattaat atcttgtgac
     1321 cctcaatatg gaattcataa agtagtaaaa ggtttaaaag gctttagttc gagagtatta
     1381 agagagaact ttccacattt aaagtctagt atgcctagca tgtggactaa tagctatttt
     1441 gtaggtactg ttggttctgt gagtttagaa gtagttaaac aatatataga aaatcaacaa
     1501 gttaggacaa agaaatgata aaaactcata gagtgaaatt aaacctaact agaacacaat
     1561 ttgaattagt aagggaaaag caaatggaaa gtgctaactg ttggaactac atagttaact
     1621 tatctaaaga atactatttt gaacataaac aatggattaa aaagaatgat atacaaaagt
     1681 taatcaaagg taagtacaat cttcatagtc aaactattca agctatatca gataaatttg
     1741 atgcaaatag aaagactata tcagaattaa gaaaaaaagg taatactaaa gcaaaatatc
     1801 catacaagac taagaaattt tatataatac cttttaaagc tagtgctatt aatagaaatg
     1861 ctaaaggcaa cttaaaatta tcaatgtcaa aaggcaggta tttagaatta gattttaatg
     1921 ttgagaatat aaaaactgct gaaatagtat ggcgaaatgg atattattta tactacactt
     1981 ttgacaatga attaaatgga gttgtagcca aaggcactaa taccgtaggt gtagacttag
     2041 gggaaataca ttcgatagct agtgtaacaa atgaaggtgt agggcttatt ctatctaata
     2101 gagaaggcag aagtattaaa caatttagaa ataagatgta tgcttatata tctaaaagat
     2161 taagtaaatg taaaaaaggt agtagacaat caaagaagtt atggagatta aaaaataaga
     2221 taagaagtaa aacagataat cagttaatga atttatatca tcaaacaact agaaagttta
     2281 tagacttttg tgtagagcag aaggtatgtg aaatagttct tggggatata aaaggggttg
     2341 aaaaagatac taagaaaaag aaaagattaa acagagttaa tagacagaaa atatctcaaa
     2401 tggagtatgg tcgaataaag gactatataa aatataaagc aaaagagcaa ggtattgaag
     2461 ttaagttagt taaagaaaac tacacttctc aaacttgccc aaagtgttct aaaaaacaca
     2521 aaccaactgg tagaacttat agttgtactt gtggttatga aactcataga gatatagttg
     2581 gtgcttggaa tattttaaat aaaaagcata aatatggttt agtagatttt agaataaatc
     2641 ataagcaacc aataaattta aaagtatcta ccgtttgagc catgtgtggg cgaataactt
     2701 taaggtagta gtggcgaaga caccgtccga agtagcttgg ctttgtaacc acctctgttg
     2761 tactgtttaa gtataaataa gtgtgttagc caccaagaat ccccttgctt tagctatggg
     2821 gagtgtgtca ataaaatact ttaatgaaaa atagctaact ttaattgtag gatattctta
     2881 atatatttga aataaataaa aatattgaag gatataatat tcaattggta tataaataat
     2941 aaatgatagt taaattttac cttagaggct aattaggtgt ttagtcaaag ctaatgcctt
     3001 tagaatacaa atgtattcta aaggcatttt tactatgtat tttcatttat attactaaac
     3061 attatctatt tttatataca tttctatgta ttttattttc atcaaatcgg tctataaact
     3121 catttttttc ttcactatat ccaatagata taagtgcata aggaactaat tttaatggta
     3181 agtctaaagt ttgtctaact ttatttacca ctttattttt aggaaatact cctgcccaac
     3241 aagcagctag accttgatta gtaacttcta ataataaatt ttgtgtgcaa gcacctaaat
     3301 cttgtatcca tttaccaggt tttaagaatt tatctctatt tccaagcact actatacata
     3361 atggtgcaaa ttctatatgc tttgctctat gctgtgactt tgagagctgt ttcagcagtt
     3421 ccttatcatc tactataata aattcccaag gttgagcatt ttttgatgaa ggtgcttgca
     3481 tagctgattt tagtatttca gttattttat cttcttctat atttttatct atatatgttc
     3541 ttattgaacg tctatttttt ataacttcta acatacagat tctcctttaa ataaaattat
     3601 ttctatttat ataatatcat tactttaaat tttagctatt acttatataa agaataaact
     3661 ttgtgttggt tttatctaag taaaagtaac attcagaact aaaaaacaag actataaatc
     3721 caaagtatta attaaaaaag aagtaataaa aaataatata caagatttaa aaaagagtat
     3781 tgggcttaaa atttaattag aaaaccaatt ttgagataat ctgtttttta ttttaacaaa
     3841 aaaacacaaa taaagttcga tacgtagcga atgaaaaaat tacaagaaat atttttttta
     3901 aaaaatttca aaaaagagtt gataatatat gtatattaat ataaaattta aggtgtataa
     3961 ttcgtataat aatacgaatg atttgtagtg taaaataact tataaacgga gaggggaagt
     4021 ctaatgaaag tagcagtagt tatgggttca aaatcagatt atccaaaatt agaagaaggg
     4081 ataaaactgc ttgaaaaata tggaatagaa gttgtagcaa gagcattatc agctcataga
     4141 acaccagaac aactttcaat attcctaaaa gaaatagaag atgatactga tgtaataata
     4201 gcagcagcag gaaaagcagc acacttacca ggtgtaatag cttcacaaac attaattcct
     4261 gtaataggat tacctattaa atcatcaact atggatggac tagactcact tttatcaata
     4321 gttcaaatgc caaaaggaat cccagttgca acagttacaa tagacttagg tttaaacgca
     4381 gctttattgg cattacaaat aatgacttta aaatacccaa aattaaaaga ggatttgaaa
     4441 tcatatagag aagagatggc acaaaaggtg ttagaagacg acaagaattt aagggggtag
     4501 cagaaatgtt attatatgaa ggaaaagcaa aacaagtata ttctacagat aatgaaaatg
     4561 aatatgttgt ttattataaa gatgatgcaa cagcatttaa tggagaaaaa aaagcagaaa
     4621 tatcttcaaa aggtatacta aataataaga tatcaacaat aatctttgaa atgttaaaag
     4681 aaaataatat aaatactcac tttataaaaa gtttatcaga tagagaaatg ttagttaaaa
     4741 aagtagagat tttaccatta gaagtaatag taagaaatat tgctgctggt tctatctgta
     4801 aaagagttgg tcttgaagaa ggtgtagttt tcgatgagcc aatttttgaa ataagctaca
     4861 aaaatgatgc ttatggtgac ccaatgctaa atgatgatta tgcagtggca atgaagttag
     4921 caactagaga agagttaaaa ttcttaagag aagaaacttt aaaaataaat gagttactaa
     4981 aagcattttt cttaaaatta aacctaaagt tagttgactt taaaatagaa tttggtaagg
     5041 attctgaagg aaatataata ttggcagatg aagtatcacc agacacttgt agactttggg
     5101 atgtaaatac aaatgaaaag ttagataagg acagatttag aaaagacctt ggagaccttg
     5161 tagaaggtta tacggaagta ctttcaagaa tgaataataa ataggaggca aatgtatgtg
     5221 tggagtttta gggatttact ctaataaaga tgtaactaaa gaactatatt attctttata
     5281 ttctatgcaa cacagggggc aagaaagctg tggtttagct cttttagatg atggagaaat
     5341 taagtacaaa aaagatatgg gattagttgg agatgttttt aaggaaaatg aattatctaa
     5401 gttaaaagga aatataggga ttggacatgt tagatattca acagctggag gaagtcatgt
     5461 ttctaactgt caacctttag ttggaagttg tagaaaaaga caattagcca tagctcataa
     5521 tggaaatcta gttaatgcaa attatttaaa agatatgcta gaagaagatg gatatatgtt
     5581 ccaaactaat tcagatacag aagtgatttt atatatactt gctagatatt acaaagggga
     5641 tatagtagaa agtttaaaag tgacaatgga ttatataaaa ggtgcatatg ctcttgtaat
     5701 aatgagtcaa gaagaattgg ttgcagttag agacccacat ggattcagac ctcttgtact
     5761 tggtaaaaag ggtgatgaat atatatttgc atcagaaaat tgtgcaatag atatacttgg
     5821 cggagaagtt ataagagatg tagagccagg agagataata gtagtcaaag atggagaact
     5881 aaaatcatat ttttattcag aaaattataa accagttaag aaaagttgta tatttgaaca
     5941 catatacttt gcaagaaatg atgcaactat agacaatgta aatgcatatg aatttagaat
     6001 taaatgtggt gagagattag ctcagaatga aactgtaaag gcagatatgg tagttccagt
     6061 tccagattca ggttggccag gagctattgg ttatgcaaat gctagtggat taaaaataag
     6121 tgaaggctta gttaaaaata gatatgtagg aagaacattt ataaaaccta cacaagaaga
     6181 aagagaaata gcagtaaaaa taaaattaaa tcctctttca acaataataa aaggaaaatc
     6241 tataatttta gtagacgact caattgtaag aggaactaca tctaaacaat tagtaaaatc
     6301 tttaagagaa gcaggagcaa aagaaattca tcttagaata acttctcctc cagtagctta
     6361 ctcttgttac tatggaatag atactccaaa tcgctctaaa ttaattgcat caagtaacaa
     6421 cgtagaagaa atgagagaat atattggatg tgatagttta aaattcctag atatagaagg
     6481 aatgttagat gcaacagaac ataagtctac tttctgtaaa gcttgttttg atggagaata
     6541 cccagttaag aaaattgata aggaggaact actttcatgt taacatataa agaatcaggt
     6601 gtagatatag atgaaggaaa tagagcagta gaccttataa aaggtaaaat aaaaggaact
     6661 tatgatggaa atgtagttgg agatttaggt aacttcagtg gattatacag tttaaaagat
     6721 tttgtaggta tgaaagaacc agttttgctt gcatctacag atggtgttgg gactaagtta
     6781 aaaatagctc agatgatgga caagcatgat actgttggaa tagatttagt tgcaatgtgc
     6841 gtgaatgatt taatttgtca aggagcaaaa ccattattct tccttgacta tatagctctt
     6901 gggaaattag tgcctgagca tatcgaaaaa atagttggtg gaattgcaga tggttgtaaa
     6961 atgtctggtt gtgcactaat tggtggagaa actgctgaaa tgcctggaat gtatggagaa
     7021 gatgactatg atttagcagg attttctgta ggaatagcag acaaagaaaa gattgtttct
     7081 ggaaacaatg ttaaatcagg agatgtattg gttggtatat cttcaagtgg agtacacagc
     7141 aatggatttt cttttataag aaagatattt ttagaaactt acaattataa aatggaacaa
     7201 tatgttgaag aactaggaat gacagttgga gaagcacttt taacaccaac taaaatatat
     7261 gttaaattag ctttagatgt attagctaaa catgacataa aagctatagc tcacataact
     7321 ggtggagggc ttatagaaaa tataacaaga gttataccta aagggttagg actagatatc
     7381 aataaaaaat cttgggagaa gcctcctata tttaaaatga tagaaggttt taatgctgtt
     7441 gatgagagag aacttcataa gagttttaac atgggtattg ggctagtttt aatagtagat
     7501 aaagaaaatg ctgatgatgt tgtaaacttt ataaataata gagaaaatga caatgctgct
     7561 tatgtagata aaaaatatag tgaattatta gaagataaag cttatataat tggtgaagta
     7621 gtagatagtc atgaaggtgt agaattatgc taaatatagg agttttaata tctggtggag
     7681 gaacaaattt acaagcagtt atagatggta ctgagtctgg agaaataaaa ggtcaggtta
     7741 aagtagttat ctcaagtaag caaggtgctt atgggctaga gagagctaaa aatcataaca
     7801 taaaagcaat ttgtgaaact gatgaagaca agataataga aattcttaag gaaaataaaa
     7861 tagatttagt agttttggct ggttatttaa aaataatcag cccaaagctg gttaacgagt
     7921 ttagaaacaa aatgataaac atacatccat cattaattcc ttcattttgt ggagctggat
     7981 tctatggaga aaaagttcat caaggtgtta tagattatgg agctaaagta actggtgcta
     8041 cagttcattt tgtagatgaa ggtgctgata ctggaccaat cataatgcaa gatgtagtta
     8101 aggtaaatca agatgatgat gcaaagacac ttgcaaaaag agtgttagaa gttgaacata
     8161 ggattttaaa agaaagtata agtctttttt gtgaaaataa attaaaactt caaggtagga
     8221 gagtgtttat aaatgagtaa gagagcttta ataagtgtaa ctgacaagac tggtgtagtg
     8281 gaatttgcta aagagttaaa taaattagat tatgaaatta tatctacagg aaatacattt
     8341 aaaacattaa aagaaaatgg tgttaatgta atgcaagttg aagatgttac aaattttcca
     8401 gaaatattag atggaagagt aaaaacatta aatccatata tacatggtgg tatactttat
     8461 aaaagagata aagaatctca tgtagaaact gtaaatgaac acaaaataca ttcaatagat
     8521 ttagtagctg taaatctata tgattttgaa ggaactttaa aagctggaaa aagtcatgat
     8581 gaaataattg aaaatataga tattggtgga ccatctatga tacgttctgc tgccaaaaac
     8641 tataaggatg taatagtagt agtagatata aaagattatg attctataat agaaaaatta
     8701 aaaacagata caatgacatt agaagacaga aaaaaattat catataaagc tttctcaact
     8761 acaggaagat atgatgcgtt aatatctagt tattttgctg gagaagttgg agatacgtat
     8821 cctgacatat taaatttaac tttccaaaaa gaacaaactt taagatatgg agaaaatcca
     8881 catcaaaatg gtttcttata ttcacagtca aatgcaaaaa atccaatctt aaattatgag
     8941 caattaggcg gtaaagaact ttcttttaac aacttaaatg atttacatgg atgtttagaa
     9001 gtaatgagag aatttaaaga tagtgaagaa gtagtttcag tagctataaa acatgctaat
     9061 tcatgtgggg taggtttagg aaaagatgct tttgaagcat atacaaaatg ttatgaagcg
     9121 gataaggttt caatatttgg aggaatagta ggaataactt ctactataga taaagctaca
     9181 gcagagaaat taaatgaaat attcttagaa atagtagtag catatgattt tgaaccagaa
     9241 gctttagaga tacttaaaca aaagaaaaat ctaagaatat taaaattagc taaaatagaa
     9301 aatagtttac aaccatatga aatgaaatat ttagatggaa aattattgat acaagataga
     9361 aacaatatat tagcagaaaa aagtgaaaat gtaacaaagg aaaaaccaac agatgcacaa
     9421 cttaaggaca tggaatttgg tatgagagta gtaaaaaata tgaaatcaaa tgcaattgca
     9481 gtagtaaaaa atggacaaac tctagcttta ggttgtggac aaacatcaag aatttgggca
     9541 ttaaaaaatg ctcttgaaaa taacaaagat aaagatttta caggagctgt tttggcgtca
     9601 gacgcatttt tcccatttga tgattgtgta acattagctc atgaatatgg aatatcagca
     9661 gtagttcaac caggtggctc tataaaagat aaagattcaa tagaagcttg tgacaagtat
     9721 gatatggtta tggtatttac aggaataaga cacttcaaac actaaggagg atattatgaa
     9781 gatattggtt gtaggcggtg gcggaagaga gcatgctata tgctggaaac taagcaaaga
     9841 aaaaaatgta gaaaaaattt attgtgcacc aggtaatgca ggaattgcaa acgtagcaga
     9901 atgtgtaaat ataggtgata ctaatataga agaactttta aaatttgcca aagaaaatga
     9961 aataggtctg acaatagtag gaccagaagt accactagtt atgggaatag tagatgagtt
    10021 tgaaaaagaa ggattaagag tatttggacc taataaaaaa tgtgctcaac ttgaaggaag
    10081 taaagctttt tcaaaagaat ttatgataaa acataacata cctactgcaa aatataaaga
    10141 atatacaaac ttagaagaag ctatatcaga aatagatagt tttggatatc cagtagtaat
    10201 aaaagcggac ggattagctg ctggaaaagg tgttgtaata cctgaaaata gagaagatgc
    10261 tatagcaact ttaaaagaaa tgatgtcaga taagaagttt ggagctgctg gagataaaat
    10321 agtaattgaa gagtttttaa aaggaataga aacttcaatt ctagcatttg tagacaatga
    10381 tacaatagtt cctatggcaa gtgcaaaaga ccataaaaaa gtaaacaact atgagcaagg
    10441 tccaaacact ggaggaatgg gaactttttc acctagtgaa atatatacag aagaattagc
    10501 aaataaagta aaagagacag tattagaaaa aactttagaa ggtttcaaaa aagatggact
    10561 taattttaaa ggtattttat ttgttggtct tatgataact gaagatggag aaaaagtact
    10621 tgaatataat gtaagatttg gagaccctga gacacaatct gttttattta gattagaaac
    10681 agatttacat gagataatgg aagctatact agataataag ttaaaagata tagaaataaa
    10741 ctatagtgat gatgaagctg tatgtgttat gcttacatca ggagggtatc cagacagtta
    10801 tgaaaaaggg aaaattataa ctggtcttga aaacttggat gatgatatag tagtattcca
    10861 tagtggcact aagatgtttg atggtaattt ggttaccaat ggaggaagag taattggtat
    10921 aactgcaaag tcaactagag ttaaggatgc agcagaaaaa gtttatgaaa acataaagaa
    10981 gataaacttt gaaggaatgc actatagaac agacatagga agataatttt tctaaaatgg
    11041 aggataaggt atgttgaata ctgaaaataa agactcaatg gttagaagag tattggttga
    11101 aaaaagagaa ggttttgacc ttgaggctaa agctttaaaa aaagacttag tagaaagttt
    11161 acacatagat aatatagaaa atttaagaat attaaataga tatgatgtag aaggaatatc
    11221 agaagaagtt tatgaaaatg cagcaaagac aatattctct gaaccaaatt tagatgtagt
    11281 atattatgaa gaaataccaa aattaaatga tgaaagagta tttgcaatag aatttcttcc
    11341 tggtcaatac gaccaaagag gtgattgggc agctcaatgt gtacaaattg taaatcaagg
    11401 gataagacct gctattaata cagcaaaagt ttatatatta tcaggcaaaa taactgatga
    11461 agagttttct aaaataaaag attactgtat aaatccagta gatagtagag aagcatcttt
    11521 agagaaacca gaaacgttaa aaatggaaac tgaaatacca acaacagttg aagtattaga
    11581 tggatttata gatttagatg aaaatggatt aagaacattt gtaagtgaaa aaggattagc
    11641 aatgacttta ggagacttac aacacgttca aaaatacttt aaagatacag aaaagagaaa
    11701 tccaactata acagaaataa aagtattaga tacttattgg tctgatcatt gtagacatac
    11761 tacttttatg actgaaatag aaaatgtaaa gatagaagat ggtaagttta atgatatagt
    11821 aaaagaagcg tatcaaatgt atcttaactc aagagacaat gtatatgtga acagacataa
    11881 agatatatgt ttaatggata tagccactgt tgcagtaaaa gaattaaaga aaaatgggaa
    11941 attaaatgac ctagatgaaa gtgaagaaat aaatgcatgt agtataaacg tagatgttga
    12001 agttgatggt aagatggaaa aatatctagt aatgtttaaa aatgaaactc acaaccatcc
    12061 aactgaaata gaaccatttg gtggagcagc tacttgttta ggaggagcta taagagaccc
    12121 attatcagga agaagttatg tttatcaagc aatgcgtgtt acaggaagtg ctgacccaag
    12181 aacgacttta gaagatactt tacctggaaa acttatgcaa agaaaaataa ctacagaagc
    12241 tgctcatgga tatagttcat atggtaacca aataggtctt acaactggac aagttgcaga
    12301 agtatatgat gaaaactttg ttgcaaagag aatggaaata ggtgcagtta tagcagcagc
    12361 accaaaagag aatgtagtaa gagaaagacc tgaagcagga gatgtaatcg ttcttcttgg
    12421 aggaaaaaca ggaagagatg gctgtggagg agcaactggt tcatctaaag aacatagtga
    12481 agagtcaatc ttaacttgta gtgcagaagt tcaaaaagga gatgctccta acgaaagaaa
    12541 aatacaaaga ttctttagaa ataaagaagt tgctcaaatg ataaaaagat gtaatgattt
    12601 tggagcaggt ggagtttgtg ttgctatagg agagattgct gatagcttag atataaactt
    12661 agacttagtt cctaaaaaat atgatggact tgatggaact gagcttgcaa tttcagagtc
    12721 acaagagcgt atggcagtag ccataaaaaa agaaaataaa gataaattta tacaattagc
    12781 agtagaagaa aacttagaag ctacgcatgt agctacagta acagatacag gatatttaag
    12841 aatgttctgg aatggaaaag caattgtaga tataaataga gagtttttag atacaaacgg
    12901 agtaaaacaa actactgatg ttcatgttac taaagtagat gaagaaaata cattctttag
    12961 ctctaatgaa atagtaaaag atgttaagtg tgcaagtatg aaagacaagt ttacaaaagt
    13021 actatctgac cttaatgttt gttctcaaaa aggattagta gagatgtttg ataatacaat
    13081 tggtggtaat acagtactta tgccatttgg aggaaagtat caagcgactc caacacaagg
    13141 tatggttgct aagataccag tacttggagg agaaactaat acttctacaa taatgactta
    13201 tggatacaat ccaaaggtag gaaaatggag cccattccat ggtgccttgt atgcagtagt
    13261 agaatctgtg tgtaaattgg ttgctattgg aggaaattat agtacaacaa gacttacatt
    13321 ccaagagtat tttgaaaaac ttggtaacaa tccagaaaaa tggggtaaac cattctcagc
    13381 acttttagga gcgttttatg cacaaagtaa atttgagata cctgctatag gtggaaaaga
    13441 cagtatgtct ggtacattta aagatattga agttccacca acattagttt catttgctgt
    13501 tgacacagta gatgcgaaga aagtagtatc tccagaattt aaaaaagcag actcaaaagt
    13561 agtaatgtta tgtgtaaaca aggcagaaaa tgatgttgta gattttgagg aattaaagag
    13621 aaacttagat aaagtaagag aacttataca tggaaataaa gttttatcaa catatgcatt
    13681 aggatttgca ggtgtaggtg aagcaataag taaaatggct tttggtaata aaataggatt
    13741 taagtttagt gaagaagctg aaaaagcatt tacagataat aaattatttg aggctagtta
    13801 tggaaatata gtacttgaat tagcaaatga tgatttaagt atgttagaag gatacaatta
    13861 tgttgtgatt ggaagtacag ttaaagaagc tagtatattt atcaaaggtg aagaacttgc
    13921 attagatgaa ttatataaag cacattgtag tactttagag cctatattcc caactaaaac
    13981 agaagaagta aaatcaaaga tagaaactat aagttatata tcacaaggag aagctaaaaa
    14041 atcatcatta agtatagcta ctccaagagt atttatacca gcattccctg gaacaaactg
    14101 tgaatatgac tctgcaagag catttgaaag agcaggagct aatgcaagta taagagtatt
    14161 taagaattta acttataaag acatagaaga ttctatagat acaatagtaa acgaaataaa
    14221 atcttctcaa ataataatgt taccaggtgg atttagtgct ggtgatgagc cagatggttc
    14281 tggtaaattt atagctactg tatttagaaa tcctagagtg caagaagcta taaatgaatt
    14341 tttaactcaa aaagatggac taatgttagg tatctgtaat ggattccaag tattaattaa
    14401 attagggtta gttccatatg gagaaataag agttccaagt gaaagtgctc caacacttac
    14461 ttacaataat attggtcgcc atcaagctaa gattgctaga actagaattt catctaataa
    14521 atctccttgg ttagctcaaa ctaatgttgg agatattcat aatatagcaa tttctcatgg
    14581 agaaggtaag tttgttgcca gtgaagatgt tatgagagaa ttaattgcta atggacaagt
    14641 agcaactcaa tatgttgact ttaataatga agctacttat gatattgagt ttaatccaaa
    14701 tggttcattc tatgctgttg aaggtataac tagtgctgat ggtagagtgt ttggtaaaat
    14761 gggacattct gaaagaattg gtgaggaagt atataagaat attataggtg aaaaagacca
    14821 aaagatattt gaatctggag ttaaatattt tagataaagc tttgatgttt aattgatagc
    14881 tagaatatat aatttagata tttagattta aagagatgta ataatatgat gataatcatt
    14941 taatattaca tctctttttt atttatctaa gaaataataa gttcaaccta agttaaaata
    15001 caagctgtgt tataattaat tattaagtaa aattgaatat ttattggagg gcaacataat
    15061 taatgaaaat attaataaca gggtctaatg gtcaattagg aaaagaattg gttaaccaat
    15121 tagaagcaat caatcaaagt ataaaccaac caaaatatgt tatattagct acaacaagaa
    15181 gtgatttgga catatccaat cagactaatg tagataattt tattttgcat aataaacctg
    15241 atgtagttgt aaactgtgca gcatatacaa aggttgatgc atgtgaagat aatatcgaaa
    15301 ttgcatacaa aataaatgct ttaggagtga gaaatctagc aatagcatca gaaaaagtta
    15361 atgctaagct tattcacata tcaactgact atgtattcaa tggtttttct aaatatccat
    15421 atagggaaga caataaaact gaacccaatt ctgtatatgg aaaaagtaaa cttatgggag
    15481 aaaaatttgt tgaacaattc tcacataagt actttatatt gagaacagca tggttatatg
    15541 gtaatgggaa taattttgta aagaccatga taaaactttc tttagaaaat aaagaggtta
    15601 atgtggttaa tgaccagttt ggaactccaa caagtacagt agacttagca aaaattataa
    15661 taagaatcat ggaaacagaa cattatggag tttatcatgc tacatgtgaa ggtgagtgta
    15721 gttggtatga ttttgccaaa aagatattta aactaaaaaa tatagatata aaagtaaatc
    15781 caattaagag taacgaattt aaatgtaagg ctccaagacc actatattct gtattggata
    15841 attttatgtt gaagttaatt ggtttaaatt cttttagaaa gtgggaagaa tccattgaag
    15901 aatatttaga aagatatgat ataaatgaaa agtaaatgta ttattttgag aaaagaaaat
    15961 aagatattat aaaaaatata aatatttctt ttatttatcc gataatcata atatgacaaa
    16021 aacaaattta aggatggaaa aagatgaaag gaattatttt agcaggaggg tctggtacaa
    16081 gactatatcc tataacaaaa tgtatatcaa aacaaacact tccaatatac gataaaccaa
    16141 tgatatatta ccctatgtcg gttcttatgt tggcagggat acaagatata ttaataatat
    16201 ctacaccaag agatataaaa gcatttgaag aattatttgg agatggaagt tcactagggc
    16261 ttaacataac atatgcaatt caggagaaag caaatggaat tgcagaagcg tttataatag
    16321 gagaagaatt tataggaaat gacagagtgt ctcttgtatt aggggataat atattttatg
    16381 gatatggatt tagtgaaagg ttggagaatg cagttaaaag aaaaggagca actatatttg
    16441 ggtatcatgt aagtaatcca aattcgtttg gagttgtaga atttgataag gattttaatg
    16501 tattgtcaat agaagaaaaa cctacagttc ctaaatctaa ttatgctata ccaggattat
    16561 atttttatga taatgatgtt gtagagatag caaagctagt tgaaccgtca gatagaggtg
    16621 aattagaaat aacctctata aacagtgaat atctaaaacg aggaaaacta aaagtggaac
    16681 ttctaggaag aggtatggct tggcttgata ctggtacaca taaaggtcta ttggatgctt
    16741 ctaattatgt tgaagtggtt caaacaagac agggactgta tatagcatgt ttagaagaga
    16801 ttgcatatac aaaaggatat ataagtagag aacaattaat gaaactagcc aagccactga
    16861 taaaaactga atatggtcaa tatctattaa aattggctag tgaagatatg taatatggta
    16921 gataaaattt attataaatt ttattaaggg agtttattat aatgggaaaa atcaatgtta
    16981 taaagactaa aatagaagga ttatatatag tcgaaccaaa tgtattttat gatgaaagag
    17041 gatattttat ggaaagctat aataaaaagg attttataga gcagggtctt gatatgtgtt
    17101 ttattcaaga taatgagtct agctcaagaa aaggcgtttt aagaggaatg cattttcagg
    17161 ttaaatatcc tcaaggaaaa ttagtcagag ttacagaggg agaagttttc gatgttgcag
    17221 ttgatttaag gaaagattca caaacatttg ggaaatggga aggtgtaata ttgtctgata
    17281 agaataaaaa acaattttat attccagaag ggtttgcaca tggattttta gttttatcag
    17341 aaaaagcagt atttagttac aaatgtacaa attattatta cccagaattt gagaatggct
    17401 ttatatggaa tgacaagcaa atatctattg agtggccttt aaaaggaatt gaagaaatat
    17461 ttttatcaga aaaggacaaa aaacaaaaaa gttttaaaga tatttgtaca agacgttaat
    17521 aaaaatttat tagaattaac atttaggaga aaaaatatga aaaaaatact gataactgga
    17581 ggagcaggat ttattgggag taattttata cattatatgc ttaacaagta tgtagattat
    17641 tttattgtta acctagactt gttgacttat gctggtaatt tagagacatt aatagaaata
    17701 caagacaatt ccaattataa atttgttaaa ggagatattt caaataggga gcaggtgtat
    17761 aagctatttg aagaagaaag gtttgatata gttgttaact ttgccgctga atctcatgta
    17821 gataggtcag ttgagaatcc tgatttattt attaaaacaa acattatagg aacacaagta
    17881 cttttagatg catctataaa atatggagtt aaaaggtatc atcaaatatc tacagacgaa
    17941 gtgtatggtg atttaccaat tgatagaaag gatttattct ttacagaaca atcttctatc
    18001 aatccaagtt ctccatattc agcttctaaa gcatcagcag atttattggt aagttcttat
    18061 tatagaacat atggattgtt aacaactata tctaggtgtt ccaacaatta tgggccatat
    18121 cattttcctg aaaaattgat tcctcttatg attttaaatg ctttggaaaa caaacaattg
    18181 ccagtatatg ggaatgggga aaatgtaaga gattggttac atgtatatga ccattgtact
    18241 gctatagact taataataca taaaggaaat ataggggaga tttacaatat aggaggtcat
    18301 aatgaaagaa gtaatctaga agtagtaaaa atgatattaa atcttttagg taaatctgaa
    18361 gagttaatat cctatgtaaa tgatagacct ggtcatgatt taagatatgc tattgatgca
    18421 actaaaatag agaatgagtt aggttggaag gcaaaatatg actttgattt gggtataaaa
    18481 gaaactgtta agtggtatat agaaaatgaa tcttggtgga aagctgtttt atcagtataa
    18541 ctttaaaatt tagaaagggg cgttttatat tgaatgagat aagttcacta attaatatat
    18601 tagcattgac tcaattatct ggtagtaata ctacaaatca atgtaattgt acatataata
    18661 attcatctgg atttgatatg attatgatga ccttgttaaa agcattatca caaaataatc
    18721 aacatacttc taatagctac aatttattgt ctaattctga gaatttattt aatgaacttg
    18781 ataatgtagt aaataatacc actagtagat ttatagatgt tgatacaaaa gataaaaatg
    18841 taaagttgag aattgaaaat gctgtagatc aagcatcaaa aaaatataac gtagattcaa
    18901 atcttataaa agcaataatt aaagttgaat cagattttaa tccaaatacc gtttcttctg
    18961 ctggtgctaa gggtcttatg caacttatgc cagaaaactg cagagactta ggagttacag
    19021 acccttttaa tatagaacag aatatagatg ctggaactag acatattaag gaatacattg
    19081 atatgtttgg agggagcata gaaatgggat tgatggcata taatggtggt cctggaagaa
    19141 tgaaaagtag aggagtagaa tcaatctctg acctgtataa aatgccaaaa gaaacacaaa
    19201 attatattcc taaggttatg aaatattata gagggtaggc tttattatgg gaataaatat
    19261 agataagcta aacataagtc caagtataaa tatggaggtt aaaaaactaa aagagagtaa
    19321 taaattttct gacaaattaa actctttaaa acaatcttct tcattggaag atataaaacg
    19381 aaatttggaa agtgtaaaaa aggcaggaga aaggcttgta cttactcaaa actatggcga
    19441 tattagtata tataaaaatg cagtaaaaga atacctaaaa tctgttgtag ataatatgta
    19501 ttcacttgat aaaaatagta gcttttggga gcatcagtat tataagaatg taaagattgt
    19561 tgatagtaaa cttgaggata tgactagtaa gttaattagt gaagaaaaag aaaacataga
    19621 tattgtttct actgttgaca tgatacaagg cttattgata gatatgtata gataaggtta
    19681 tacaaggaga aaaattttat gaatagcaaa aaagaggata tatatgaatt gaattttggg
    19741 caattaatag aagaaaaatc tggtgttata gaagaaaatc aaaatgtttt taatgcaaaa
    19801 gtaaatgtag ctgtttctat aggaaataca acagaaacaa taaaaaatat attagattta
    19861 aaagaaggct ctataataaa attaagtaag aatgtggatg aaaaattaga tatatatgca
    19921 aacgatagat tgtttgctta tggagaaagc attattacaa atgagaggtt atctgtaaga
    19981 ttatctaaaa tagaagatcc agatgtaaat tagcatttta aatagtatcc ctttaggcta
    20041 ttctgtatga gtttagctaa ggggatattt ttataaaaaa tttaaaatat gtgtgaatta
    20101 tataaatatt tttattttat gagcgataac taaattataa gtatgtaatt ttggaggtga
    20161 aactatggat attaaaaatg taagttcaaa tattgtagtg aataattatt taaagaatac
    20221 atctggtata aaaaaagtgg aagaggttaa aaaaattaat aaaacagata agatagagtt
    20281 atctaaattt gcaaaggaaa tggcaagtgt gtcaaatatg agtgatgaga atattagaaa
    20341 taaaaaagtc gatgaaataa aaaatgcatt agaaaatgga acttacaaaa taaaaccaaa
    20401 agaaattgcg aaaaaaatga tagaggatat gcgaggataa agtcatgatt acagacttaa
    20461 aagttataat atatgaagaa aaaaatataa ttagtaagat gatggaatta ttaaaagaac
    20521 aatttgatta tatagttgaa aaagacttag aaaatttaaa tatgttaaat cctaaactag
    20581 aagatattag cagggagctt gcatccatag aaataaagag aagacaactt tttggtgatg
    20641 atatttcaat gagtgaagtt gttgaaaata gtaatgatgg atacttaaaa gaaatatata
    20701 cagacttgaa acacattctt gctttagcta aaaatcaaca agaatcaaat gattctctaa
    20761 taaagaaaga acttattttt acgaaaaaaa tgattaattt cataaaacca gctgataagc
    20821 aaactacaac ttataattct tatggaaata ttagaaaata aatattagga ggagaaatat
    20881 ggcaggatta ttttccattt taaatacagc taattcagga atgaatgcgc accaaaaagc
    20941 aatacagaca atttctcata atataagtaa tctagataca gacggttatt ctaggcaaag
    21001 gaccgagttt gccacaaata gtccaatgta tatgcctagt ttgagtaatt caattgcaag
    21061 aggacaatta gggactggag tacatgtaac ggatgtaaca agagctagaa atagttttta
    21121 tgattaccaa tttagggcag aagctcataa gtatggtaag acagtttcaa aatatgatta
    21181 ttacaatact atagaaacta tactaaatga accatcagac tatggaatat ctgcaggtat
    21241 agatgatttt tttaaaggat gggagtcatt atcaaaggat ataaatagtg acagtaaaaa
    21301 aagtctagtg gtagaaaacg catctaattt agcaacttta ataagtgaaa gttataagaa
    21361 gcttgataag ttaaaagaag atatagattc taatatagac actgagataa aacaaataaa
    21421 tgacatgtta aaaagtcttg aagatttaaa taaatctatt gatattatat ctggttctgg
    21481 ctctacacca aatgatttac ttgatgaaag agatagaatt ttagataact tgagttttaa
    21541 actagacttt gaaaatagtg atgttaaaaa tatgcttgca gatggtaaat tagaacttaa
    21601 tgaattaaaa aatccagatg gaacttggaa aacaggtata tcagggacct tacaaggctt
    21661 gtttgaaatg catggtaaga ttgatacata taaaagcgat ttaaaagatg tatctgatgg
    21721 attagctaaa cagataaatg atgtttataa tacgtctgca ggaataacag taacagattt
    21781 ttttataact tcaaatgtag atggtgaaga tattataaag ataaatccag ctataaagag
    21841 taacccaaat gaattaaagc ttactgctga tgaagcttca aaaattgcta agttaaagga
    21901 tgaaaaaata gatattgggg tggctggtgg aaaagtaagt acaataagtg accactataa
    21961 agcctttgca gaatcaatcg gtttagattc tcaaaaagta aatcaagatg aagcaaatca
    22021 acgtaaaatt ataaataata ttgataactc aagaatgagt gtttcaggtg tatctttgga
    22081 tgaagaaatg acagagctta taaaaataca acgttcatat caagctagtg caaaagttat
    22141 gtcaacagct gtgcaactac tggatgttgt aataaatgga ataatttaat agaaggggga
    22201 atttataatg agagtcagta caggtatgat gtctagcagc tatctaaact cactacaaga
    22261 taatctacaa agattggata aggttaatag acaagttaat acaacaaaag aaataaacaa
    22321 gctatcagat aatccatata aagcaataaa gatattaaac tcaaaaagtg aaataaagac
    22381 tatggagact tatgttgaga attgtaaaga tacagggaat tggttagaaa ctactgatac
    22441 tgcattagat cagcttggaa aattattatc agacataaag aaaactttgg tggcaagtgg
    22501 tgatggtgca tactcagatg atgaattaaa gactcttagt aatgaaacta atgaaaaaat
    22561 gaaagaaatt gcaaatgctt taaatgcaac acatgaaggg aagtacatat tttcaggttc
    22621 aaatacgggg gtaccacctg ttgaatgtat agataatgct gatggttcag tgagtttaaa
    22681 gtttaattct agtctaaatc ttaataaatt aaatgactct ttgagtatgg atatagctca
    22741 aggaataagt gtagattata acgttaaatt gagtagcctt ggatttgacc caacaaaaac
    22801 tgacccattt gaagagttaa ataacatatc aaaaaaatta gcaaatccag atgctgatgc
    22861 tataaaagag cttacaacaa cttgtttggg cgatatggaa caactaatag aaaatacagt
    22921 aaatgttagg tctatatatg ggacaaaagc aaatacagta gaggctatga aggaaaaaaa
    22981 cgatgatagt ttgatacagt taaaggatat tttgtcacaa aatgaagaaa tagattatgg
    23041 tgaaaaactt gttcaattaa aggcagcaga gttaacttat caagcttcac tacaaacagg
    23101 aggaaagtta tttaatgtat ctatattaga ttacatataa taaaataacg cagatatgat
    23161 gaaggttaca ttaaaaaaag gtatattagg ttttgaaaat ttaaaagaat atgagctttt
    23221 agatatagaa gataatgatt atttaaaaga atttaattct actgaagaag aaggtatagg
    23281 atttgttgtt gtatctccat ttgagattat caatgaatat gaaatagttt taaatcagga
    23341 aacaatagaa aaactagaag tgaaatctcc aaaggatgtt gtattattta atataattac
    23401 tcttggtcag acatttgaag aatcaactgt gaacatgaaa gctcctatag tgataaatat
    23461 aaaaactaat tttggaatgc aaataatttt acaggatgat aaatattcaa taaggcatcc
    23521 attattgaga ggtgatagtg gatgctagta atttcaagaa aaaaagatga agcagtactt
    23581 ataggagaaa gcatagaagt aaaaattgta ggggtagatg gaaacaatgt gaaactagct
    23641 atatcagcgc ctaacaatat aagtatatta agaaaagaaa tatacgaaaa agtaaagagt
    23701 gagaatataa aagctacaaa taaaaatata aaaattttaa agtcattaaa ataataagct
    23761 tggaggaagt aatatatgta tggagaaaat ccttataatt cttataaaca aaatgctgtt
    23821 tttatggcat caaaggaaca attgttactt atgcttgttg atggagcagt aaaatatacc
    23881 aaaatagcca aaacagctat cttagataaa aatacacaaa aagcgcatag agagcttata
    23941 agagttcagg atatatttac agaactaatg gttacattag accaaaatgc aggtcaatgg
    24001 gcaaaggata tgtacagagt ttatgatttt gtaagatatg aactttcaag agctaatatt
    24061 agaaaagaca ttcaaataat agataatgtg ttgcctgtaa tagaacaaat taaagatact
    24121 tggcatgaag ctgataaaaa aagtaaagaa gaaagaagta ggtacaaata atggaattaa
    24181 atttagctga attagataag ttatcaaaag aggaattact attgatgctt gtagatggaa
    24241 cagtaaaata taccaatata tccaaagaag ctcttttaaa taacgactat ttgaaagcac
    24301 ataatgaact tgtaagagtt caaaatatat ttacagaact aatgactaca ttagaccaag
    24361 atgcaggtca atgggcaaag gatatgtata aagtttatga atttataaaa tctgaattag
    24421 caatagctga tgaaaataaa gatattaaaa taattgatga tatattgcca atagtaaaac
    24481 aaattagaga tacatggcat gaagtttaca atcaactaaa aatataattg gatggaggga
    24541 taaaggtatg tcaagtataa gtcctataag agttacaggt ctttcaggaa attttgatat
    24601 ggaaggcata atcgaagcta gtatgattag agacaaggaa aaagttgata aagcaaaaca
    24661 agaacaacaa atcgttaaat ggaagcaaga aatatataga aatgttatac aagaatcaaa
    24721 agatctttat gataaatatc taagcgtaaa ttctcctaat agtatagtaa gtgaaaaagc
    24781 atactcttct acaagaataa ccagttctga tgaaagtatt atagtagcaa aaggctcagc
    24841 tggtgcagaa aaaataaatt atcaatttgc agtttctcaa atggctgaac cagcaaaatt
    24901 tactattaaa ttaaattcaa gtgaacctat tgttcaacag tttcctccaa atgctagtgg
    24961 agcgagttct ttaactatag gagatgtaaa tataccaata tctgaacaag atactacaag
    25021 tactattgta agtaagataa actccctttg cgcagataat gatataaagg cttcttatag
    25081 tgagatgaca ggtgaattga ttatttcaag aaaacagact ggttcgtcat cagacattaa
    25141 tttaagagta attggaaatg acaatttagc tcagcaaatt gctaatgata atggtatcac
    25201 atttataaat gatgctggtg gaaacaaagt ggcaaatgta tatggaaaga atctagaagc
    25261 tgatgtaact gatgaacatg gaagagtaac tcatataagt aaagaacaaa attcatttaa
    25321 tatagataat attgactata atgtaaattc aaaaggaact gcaaagttga cttctgtcac
    25381 tgatactgaa gaagctgtta aaaatatgca agcatttgtg gatgattata ataaactgat
    25441 ggacaaggtc tatggtttag ttactactaa aaaaccaaaa gattatccgc ctcttacaga
    25501 tgcccaaaaa gaagatatga caactgaaga aatagaaaaa tgggaaaaga aagctaaaga
    25561 aggtatactt agaaatgatg atgagttaag aggttttgtt gaagatattc aatctgcatt
    25621 ttttggagat ggaaaaaata ttattgcatt aagaaaacta ggtatcaatg aaagtgaaaa
    25681 ttacaataaa aaaggtcaaa tatcatttaa tgcagatact ttttcaaagg ctcttataga
    25741 tgatagtgat aaggtataca aaacactagc aggttattct tcgaattatg atgataaggg
    25801 aatgtttgaa aagctaaaag atattgtata tgaatattct ggaagttcaa cttctaaact
    25861 tcctaaaaaa gcaggtatag aaaaaactgc ttctgctagt gaaaatgtat attcaaaaca
    25921 aattgcagag caagaaagaa atataagcag gttagttgaa aaaatgaatg ataaagagaa
    25981 aagactttat gctaaatatt cagccttaga atctttgttg aatcagtatt cttcccaaat
    26041 gaattatttc tcacaagcac agggtaatta attatggata aattaaaaga aaaattaaat
    26101 ttatataaag atatatcttt acaaattata aatttgattg aaaaggaaga atatataaag
    26161 ataagtatta agttaggtga aagacaagag attataaata gtgtaagtga aattgataga
    26221 aatgatttta tacagcttta taatagaatg gaattaattg aaattgatag caggataaga
    26281 gatattttac aaggacaatt attagaagta aaaaaagaat tacatgagta taagcttact
    26341 aaacaagtga acacaatgta ttataactta aatagagaaa aagtaaatat ttttaataaa
    26401 aaagtataaa gttatgtacc atcttgtccg attatataaa tgaagaatta ataaaaaaga
    26461 ttaaaagtag aaatgacaag gatgtcaact atactaagga gggtaaaata atgagagtta
    26521 atacaaatgt aagtgctttg atagcaaata accaaatggg aagaaacgta aatgcacaaa
    26581 gtaagtctat ggagaagcta tcttctggtg ttagaattaa gagagctgct gacgatgctg
    26641 ctgggcttgc tatatctgag aaaatgagag ctcaaataaa aggtttagac caagcaggaa
    26701 gaaacgttca agatggtata tctgttgtac aaacagcaga aggtgcatta gaagaaactg
    26761 gtaacatact acaaagaatg agaactttat cagtacaatc ttcaaatgaa actaatactg
    26821 ctgaagaaag acaaaagata gctgatgagt tactacaatt aaaagatgaa gttgaaagaa
    26881 tatcaagctc aattgagttt aatggtaaga aattacttga tggaagttct acagaaataa
    26941 gattacaagt tggagcaaac tttggaacaa atgttgcagg aacatctaac aacaacaatg
    27001 aaataaaagt tgctctagta aatacttcaa gtataatgag taaagcaggt ataacaagtt
    27061 ctacaatagc aagcttaaat gcagatggaa cttctggaac aaatgcagct aaacaaatgg
    27121 tatctagctt agatgtagct cttaaagaat taaatacatc aagagcaaaa ttaggggcac
    27181 aacaaaatag actagaatca actcaaaaca acttaaataa tactatagaa aatgttacag
    27241 cagctgaatc aagaataaga gatacagatg ttgcttcaga aatggttaac ttatctaaaa
    27301 tgaatatatt agttcaagca tcacagtcaa tgcttgctca agctaatcaa caaccacaag
    27361 gagttttaca attattagga taaaataatt ttgtatattg gatatagaag aaagggtaag
    27421 gctttgcttt actcctttct ttttttacaa atatactttt gaaaggaaaa tttatatgtt
    27481 acttagtata gttatgattg ttaaaaatga agaaaaaata cttgaaaaaa ctttaaaatc
    27541 attaaccaca ttaagaaatt ctatagaaag tgaacttata atagtcgata caggttctac
    27601 agatgatact ataaaaatat ctaagaagta cacggaaaaa gtgtattttc ataattggaa
    27661 taatgatttt tctagtatga gaaatatatc aatatcctat gctaaaggtg aatggttact
    27721 tatattagat gcagatgaaa tactaattga ttcttcaact attgtaaaat tttttaaaga
    27781 tggtcttgat aaaaaattta attctgcttc aatacaatta aaaaatcttt actcatatga
    27841 taaaaaatta tatggatatt gttcagtgct tagacttttt aaaaacgttg attttagata
    27901 ccatggtaaa gtacatgaac aaccaatata caaaaaccct atatttaata atgttgctga
    27961 ttttgaacat tatggatatt tgtttgaaga tgaagaaata agattaaata aggtgaaaag
    28021 aaatgaaaaa ctattatttc aagagttaaa agagaatgat aattctccat atacaaacta
    28081 tcaattaggg aaaaatttta ttatattagg aaaatatcag gaggctttat actatcttga
    28141 gaaatcttat gaattatatt ctaaactaga attggcacct ggttacttga taacaagcct
    28201 tgtaaaaaca tatttatatt taggaaaaca taagaagtgt gaaagattgt gcttaaagta
    28261 tattaagaaa gacatgaata atattgatat atattattat attgcacaag cacaagtaga
    28321 tttaggaaag tatgaaaata gtatagattc gtataagaga tatatttact tgttagataa
    28381 ttatgaaata agtacacaag caaatagttt attttcagac actgatactg tagggcttag
    28441 agatgaggct attattacac tgataaagat ttattataag cttgaaaaat atgagtttgt
    28501 aatttcagag tataataaca ttgaagatgt ggaaaagaaa aagaatgtgt actttagctt
    28561 atttatgtca ctatataaat tgaatagatt tgaagatata aaaaattatt ataaagaagt
    28621 ttcaaattct aaaattgaaa aaaatagttt ttatagaaat atagaagaag ttataaataa
    28681 tattaaagag gatgaaaaag agtatgtata taaaatcctt tgtgacattg aaggtaatta
    28741 tggcatttta aatcaaataa ggataaataa atgtgtatct atagataaat gtaaagaaat
    28801 actcaatgaa gaaaaggaaa taatttatgc atatattata aaaatagctt ttgaagagaa
    28861 gattgattta ctagatattt tttatgaaat ggaatatgta tggatagaga aatatctaaa
    28921 atatctttta gcattagaaa gaagttttaa tttagtacta tataagtaca taattagtcg
    28981 tccaaataca tcagatatgg aaaagataag agtatataaa gctatatcta aagtagtttt
    29041 agaaagtata agcttaagtg atgaaaaata taaggagatt ttttatcttt atataatgta
    29101 ttcttataag tacataagat acatatatag caatttaagt gattttgaat taataaaatt
    29161 tgtttgtaat gatttagata agttcactat agaatttaag catctacttg atattaaaaa
    29221 taaagtaaat aatgctaatg aaaaattaga gtatatacat agtatgagga aattactaaa
    29281 tgagtatcct ttttataata aaataataaa aatacttata ggtgaattag aagaaaatat
    29341 aaaagaaagt gaagaattta aggatttaaa agatagtttt attattagta tagaaaatat
    29401 gattgaaact ggcaaaataa atgatgcgaa attattggta gatgactatt caaaaacatt
    29461 tgtggatgat ataaagattt taaatataaa gggaatttta tacatgtttg aaaataagct
    29521 tgataaagct gattttatgt taaaaaaggc attatcatta gatgtagaaa atgaagatac
    29581 tatatataat atagaatact taaaaagttt aagataagtc aaaaatagga gaattgaaga
    29641 atgaatatac caatagtggt taatgaaaaa tctaaagaca taataaaaga ttggcaatat
    29701 acatgtacaa atgaagatat aaaatttaca agcataataa tattgacata taatcaaata
    29761 gaatatacta aattatgtat tgaaagtatt aggaaattta ccccaaagaa taaatatgaa
    29821 ataataataa ttgataataa ctcttctgat ggaacagttg agtggttaaa gggacaatct
    29881 gatataaaat ctgtgtataa taacaaaaac ttaggatttc caaaaggatg caatcaaggt
    29941 attgagttag ctactggtga aaacatattg ttgttaaata atgatgtaat agttactcca
    30001 aattggctct ataatttgaa taaagcttta tggtctcaag ataatatagg tgctgtagga
    30061 cctattacga atgcatgttc atattatcag caaattgatg ttgattataa gtcaattgag
    30121 gacatgttta tatttgcaca caaaataaat aaatctaata ttaacctatg gaaatataga
    30181 ttgaagttag ttggattttg catgttaata aaaaaaactg ttctggataa tgtaggggtt
    30241 ttagatgaat tattcacacc tggaaatttt gaagatgatg atttatcttt taggatgata
    30301 gagtcaggat ataagttatt attatgccag gatactttta tacaccattt tggaagtgtg
    30361 tcttttaaag aaaatcgtaa taaatataca gatataatgt atattaatag aagaaaattt
    30421 gaaactaagt ggggatttaa tccagaatat agtggaatga taagaacaga tttgatttct
    30481 aaaatagatg atagtttgga ggcaaatatt aatgttttag aaataggatg cggtataggg
    30541 gctactcttt tagaaataaa aaatatatat aaaaatgcat caatatatgg aatagaaatt
    30601 agtgagtcag caggtaaact tgcaaagtat agtgcagatg tacttatagc aaatatagaa
    30661 aaagtaaaat tggattataa aaaagatttt tttgattata taatattggg agatgtactt
    30721 gaacatttga ttgacccatg gaagatagtc aatgaattga aacaatatct aaagaaggat
    30781 ggttatctgg tatctagtat accaaatatt atgcatgttt ctgttttaag aaatcttgtg
    30841 ttaggtaatt ttacatatga agattcagga attttggaca agactcattt aaggtttttt
    30901 acattaaatg agataacaaa aatgtttaat gatagtaatt acataattaa tagtattaca
    30961 gctactagta tgccactttc ggaaattgat aatagcttca ttgatacaat atgtaaggaa
    31021 agctgtaaag aaatgagaca acagtacgaa tcttatcaat atatagtaaa agcctctaaa
    31081 atcctagata ctaaaagata ttctagtatt agtatgatta atttaaaata cgctttaatg
    31141 agaattgata atgaattaga tgtagatagt aatttaagtt atatatttga taattataat
    31201 ataactgaac catgttttgt tgatgatatt gaatatctaa ttgatacatt tgtgataaat
    31261 aaagcaactc tattaaatag attaggagtt gaggcattta acagaggtct aggagatttt
    31321 agtataaaca tgtttatgac agtattagag atggatagag atgatattga ctcaatatat
    31381 aatataacaa gtgtattatg tgaattagaa gaatttgata ctgcttataa tttaattaaa
    31441 aatagtagcg aaaaagtaca gagtgaaagt gatatacaag agatactttg ttttattgag
    31501 gggaagaaat atgaataatg ataagatatg ttttataacc tgtgtaaatg atgaaaggct
    31561 ttatgaagaa tgtcttgtat atataaatag cctagacatt cctaaaaact ttagtataga
    31621 aacagtagct ttaagaggag ccgattctat tgcctctgct tataataaag ctatgagaga
    31681 aacagattct aagtataagg tatacttaca tcaagatact tttataatta ataaaaattt
    31741 catacatgat attttagaga tatttaaaaa aaatacagat gtcggaatga ttggtgtagc
    31801 tggagtaaaa gatatgcctg tgaatggaat ttggtgggat tctgataaaa aagcagggaa
    31861 agtatatgat agtcatacag gtaaaatgga tatttatgaa tttagtaact ttgaagaatg
    31921 ttttaatgaa gttatggcta tagatggatt aattatgatt actcaatatg atgtattttg
    31981 gagagaggat aagtttgatg ggtggcattt ttatgatttg tcacagagta tggagtttag
    32041 acaaaataaa tacaaagtta ttgtaccaaa acaaaataag ccttggtgta tgcatgattc
    32101 tggagtagta aatataaata atggatatga tgaatatagg ataaaatttg taaaagagta
    32161 cataaatctt gatttatata gagaaaattt ttatgaattt gataagaaga aagcaagtac
    32221 catacttaag agtaataagc atgtaatgga ttgtatgaaa gaggagctta cagaacaatt
    32281 aagattaaag aattacaagg atgttttagg tattgtttct aaaattgctc atttttctgt
    32341 ttttaatcat actggtttgt attcttgtga agaatgtgag agagcattgc tagaatgtgc
    32401 aaataattta ccagaaatag aaaatacgaa attatttaca aaaaaagatt taaaaagaag
    32461 aaaagtatta catgttttaa gtgaaggata ttcaacagga ggtcatacaa gactagttaa
    32521 aaattggatt gaaattgaca aagactctgt tcattcagtt gtaactacat ggcaatcaga
    32581 tacactgcca gactggttat taaaagcaat aaatgatagt ggtggcgaag ttatatcttt
    32641 ggatttaatt tcagatgatt atttaaaaag agctgtagct ttgaggaaaa tagcttatga
    32701 ctgggctgat atagttattt tatatgttca tatgtgggat acaatacctg ttgttgcttt
    32761 tggagttgat gggaaaatac ctattttaca tgttaatcac tatgatagta ctttttggtt
    32821 agggacaagt attgcagata ttgtcattga ttttagcgaa actagtcaat taattacatt
    32881 agataaaaga ggcatagata aatctgaaat attaccaatg ccattaaaag aaaaagaagt
    32941 attttcaaaa tctgaagtta gaaaacgata taatttagag gatgatgtga caataatatt
    33001 gactattgct agtgaaagaa aatacaatag tgtaaatgat attaattact ttaaaattgc
    33061 taaaacattg attgaaaata cagataatac aattattatt gttgtaggag ttgatacaga
    33121 gaatctctat tggaaaactt taagtaatgt tacagataat agagttatat gttttggaac
    33181 aacagcaaat atagatgatt tttataagat ttcggatata tacctagagt ctttcatggt
    33241 aggaagtcat acttcaaaat tagatgcaat tttatatgat ttaattcctg ttaagtttaa
    33301 aaatgaagtt tcaccaattt ttacaaatat gtggaatttt atagataagt ttgattataa
    33361 gaatataaaa gaggttatag acatgataag aaggtacaat actagtgaat ttgacaggga
    33421 taagataaaa gcaaaacaat cggaaataaa acattttgtt atagataaac attgtattga
    33481 aataaaaaac tatatagaca aaatatataa tgaagttaat catcataaag ttaagtatga
    33541 tttatataaa aatgatgaaa tcgaggacta tcagcttttt gtagcattat atggctatat
    33601 ggtaaaaaat aaaaacatgt cataggtggt aaaatgaaca atattcatat caataaattt
    33661 gaaaaaagct gtgaaaaaaa ctgggattat tttgtagaaa ataaatctat aaatggtaca
    33721 tttctgcaaa ctagaaattt ccttaattat catgaggagg gaaaattcac tgaccactca
    33781 ttaataatat gtaaaggtga agaaatacta gcagtcgtac ctgcatgtga aacttatgaa
    33841 gatggtgaaa gaatattttt ttctcattta gggagtactt ttggtggcat tataataaga
    33901 agtggtttta atgatattag gcatgtaaat gccattacaa ataagttaga aatatattta
    33961 caggaacaaa atttcgataa agtatattta aaaaatacat caagaatatt ttgtaaagag
    34021 gatacttcat tactagaata ttttttattt aagaataatt acatgtttta tgatgaatta
    34081 agtatgtttt tagaattaag taagtataaa gaagatatat tagccaattt taaatacagt
    34141 aaaagaagag attttaaata ttctttgaag aataatctta cctttaaaag actgtatagt
    34201 gatgaagata tagtttgttt ttttaattta ctttctaatt gtttagatag taaaggagct
    34261 aaagtaatac attcagtaga agaacttttg gagtttaaaa ataatagatt aaaagatatt
    34321 gtagaatttt atgcagtata tattgatgac attatgattt gtgggagcat ggtatttaac
    34381 tttcaagaaa gagtttatca tactcaatat ctagcatcaa attcgacata taataaatat
    34441 tatccaatga tatttttata ctataatcta ataaaagatg ctttagagag aaattttaag
    34501 tatctttctt ttggaattag cactctaaat agaggcaaat acttagatga gggtttagct
    34561 acatttaaag aaggttttgg ttcaagtgca atttgtaaca gaacatacta taaaaatata
    34621 aataagtgag aatcatgttt tatagagttt ttagagtata gtaaatatct agtactataa
    34681 tattacataa aaataattag tttataaaga aggtttgtat atgaaaagat taatgatttt
    34741 aggctcaatt aagtattttg agaatattgt tatgtcagct aaaagagagg gaatatacac
    34801 aattgtttgt gataatagaa ttgatactcc tgcgaaaaag atttgtgatg aagcaataga
    34861 tgttgatgta tttgattttg ataaaataaa gaatatagct atagataaaa agatagatgg
    34921 aattttaact ggatttacag attctttaat gaagccatat gtttatattg caaatgaact
    34981 aaatttgcca tgtgtgattt cttgcaatca acttgaatct gtaactaata aggctgttat
    35041 gaaaaattat tttaaaaata atggaatacc tacaactgat tattgtatta ttgaaagttt
    35101 gaaagattta gaaaaaatac aaggattaaa ttttccactt gtaataaaac ctgttgatgg
    35161 ttatggttca agaggtgtat attttgtatc aaacatagaa gaattaatag aaaaatttga
    35221 atattctaag gaagcctcgc aagatggaca agtaataatt gaggaatttt atgaaagtga
    35281 ggaaatacaa ggactagctt gggttcataa tggagagtca catgtatttt atattggaga
    35341 tagagagctt gtaaatatac atacaggaag agctggaaaa ccagatagat taatttatcc
    35401 atcaaagtat tgttttcaat atgaagaaga aataaaatcc atatatcaaa atataacgca
    35461 ggcttttggc ataaaaaatg gacctcttta tgtacaaatg ttagtgggag catatggaat
    35521 aaaggtatct gaagtaatgc caagattacc tggaggttgt gactatatgg ctatttctca
    35581 aataacagat ttagacatag gaagtttatt tgcaaacttt agtgtgaata atgaagtaaa
    35641 ttttgatgaa ataaagaaat ataatatgca tctatcaaaa tgtgtatatg cattaccaat
    35701 atacataaga cctggcacaa taaaaaagat aaataatata gataaaatag aaaacttaga
    35761 ttatgtcact caatttctat tactagtaaa agaaggagat acaataggat tatatgaaga
    35821 tgagagacag gattgtggaa gagtttatgg agtagcaaat aatgtatttg atgcaaatat
    35881 aaagaagaaa tgtattcatg aaatgataga aatacttgat gaaaataatg aaaatatgat
    35941 agaaaatttt taaataaggg agtatgatag atatgaagct aataactcat gaacaaatta
    36001 tgtcattaaa catttctcag aaacaaggat acgagtggat aaaagaatcc tttttaacaa
    36061 aaaaagattg tcaattacca ccaaaaataa gcatggtaga agaagggcat gtattttaca
    36121 atgtaatgcc gtccctaaat cataatcata agattggtgg aataaaaatg gttacaagat
    36181 tgccagggag aaatccaagt ttaaattcag agatatttct atataatatg gaagatgcta
    36241 aattaaaggc tattttagat ggcaattata ttacagcttt taggactgct gctgttgctg
    36301 ccttggctat cgatacattt gctaaaaaag attttaatac tattgctatt atgggtttag
    36361 gtgtaactgc ttctgctata atgaatatgc tttcatatac tcttaaagat aaaaatataa
    36421 atataaagtt acttaaatat aaagaccaag cagagtcgtt tgtaaaaaag tatgaaaaaa
    36481 atgaacaatt tagttttgaa atatgtgaca cacatgaaga acttattgta gattcagatg
    36541 tagttatttc tactgtgaca tatacagatg aatatttatc agaatttaaa tggtttaaaa
    36601 aaggtgcttt attattacct gtacatgtta tgggatttgg aaatgttgat tatctatttg
    36661 ataaagtata tttggatgat tataatcatt tgaaacatct agaaaatata gatagatata
    36721 aatattcttg tgaattttat gatgttttaa gtaaacaagc taaaggaaga gaaaatgatg
    36781 atgagagaat aatatcttac aatataggtt tgtcaataca tgatatatta tttgcaaata
    36841 aaatttacga attgtgtgaa acactaagct gtgaagaaat agaattaaaa gaaccaaaag
    36901 aaaaatattg gatatagttt atttcattca tatttacaat agtttattgt aaatatgaat
    36961 atgaagtaat atattgttag aaaattagca tgaataggat agataaattt atggataaat
    37021 ataatttaat taggttcgta tctattggaa acgaatatat aggaaattta gttgcattag
    37081 aatcaaataa agcaatacct tttgatataa agagagttta ttacatatat gatgtaccaa
    37141 acaatgtaaa gagaggattt catgcacaca aaaacttgga acaagtacta gtttgcctaa
    37201 atggaagtgt gaagataaaa tgttatgatg gtgtaaaaga gactatattt aatttacata
    37261 aaaatgatgt tggattatat ataggaacgg gagtgtggca tgagatgttt gattttgatg
    37321 aaaaaactgt tttaatttct atagcatctg aacattataa tgaagaagat tatattagag
    37381 actataaaga atttttaaag tatttaaata atgtgtagtt attatacaat tagtgatgta
    37441 ttttcttaat taaataaatt taagatagcc acatagagaa tagatagatt aggatgtgaa
    37501 tttattgttg gttaaattta ataatttatt aaaacagttc caaactcata aagaagaata
    37561 tgaacagaag attaaggatg taatggaaag tggatattat atccttggaa atgaagtaaa
    37621 gcattttgaa gaagaatttg ctaactatgt aggtagcaaa tattgtgttg gtttagccag
    37681 tggtttagat gcactgataa ttgcttttag agcattagga ataagcaaag gtgatgaagt
    37741 aatcgtgcaa tcaaatacat atattgctag tgttatggga atcagtataa atggagcaac
    37801 accagtattt gtagaaccaa atgaatatta taatatagat tcatctaaga tagaagaaaa
    37861 gataaatagc aaaacaaaag ctatattagt agtgcattta tatggtcaag caagtaatat
    37921 gaaagatata caagaattat gtgataagta taacttgtat ttaattgaag attgtgctca
    37981 atctcatgga gctaaatatg atggaaaaat gacaggaaca tttggtgata taggttgttt
    38041 tagtttttat ccaagtaaaa atttgggtgc atttggagat gcaggagcta tagtaactag
    38101 taatgaggaa atagctgata aagtgaaaat tcttagaaac tatggctcag aaaagcgata
    38161 ttataataaa tttgtcggat ataattcaag attggacgaa attcaggcag gtttattaag
    38221 agtaaaactt aaatatctaa atgaaattga aattgaaaaa tcaaaaatcg tagatagata
    38281 tcttaatgaa attaataatc ccaaagtcat acttccaaag atagaaaata attgtactca
    38341 tgtatggcat ttatttgttt tacaagttga aaaacgagat acatttatgg aatacttaaa
    38401 gaaaagtggt atagagtcta taatacatta tccgatacca ccacatctat ctgaagcata
    38461 tgcttatttg ggatataaaa aggggagttt taatatagct gagaactatt cagataaagt
    38521 tgttagtata cctttattta ttggtatgga agatgaagaa gttagttata taattaaaat
    38581 tataaatgaa tattaaacta ctcataataa tatcaaaaat aaatttttta tatatcattt
    38641 atttaaattt cgttaattga catttttaaa tattgatata ttgtacaaat aaaattgaaa
    38701 tatatggaaa aatatagaat taagatgaga tttgtcttat atttttataa attaaaaata
    38761 ttttcaaaga gtttattata aaattgtaaa ctatagtgtt tataatcttt gtaattattg
    38821 gtaattaaat ttaaaaaata aaatatttaa ttatacacaa aaaagatatt taaaaaattt
    38881 gttatttatt gtatacttaa gttaaaacta aataggcaaa tctagagaaa tctagtgacg
    38941 caaagctata gggactaagg tttatataca taaactatgt cagccagttg ccaaaaagag
    39001 tcctaggtgt attgtatacc taagaaaagt ctataatgac tgcctttttg gcagtcattt
    39061 tgttatggaa aaatcacttt atttcagtag aaaatacaca taataacata ttttaccaat
    39121 tataacataa aataaagaaa aattatctca aaaagttact attttacaaa aaatgacttc
    39181 tttatttatt taaattaatc gcatttccat tacattaata caataatgta tcaatttagt
    39241 aagaaaacta atattttttt aatgaagtcc catgaaaata taaaaaaatt ttaatatgta
    39301 tttaaatatt tcttcgaaaa gttgctcttt ttgtaatata gcaacttcat tttttttatt
    39361 aataaacaaa atagataaaa tatattttga ggaggggtga gtgtgaatac atatgacatt
    39421 ataaaaatgg gattagatgc aactaatcta agaagtcaga caatagcaaa taatatagca
    39481 aatataaata ctgaaggata taaaagacat tatataactt ttgaagaaac actaaatgat
    39541 gctatgccta aaatagaaga aaaaacagac aattctaaag gtttaagagt agatggtaat
    39601 aatgtagaca tagaagaaga aaaagtaaat caagcaatgg caacattaca atataatgct
    39661 ttgataagtt ttgcaaatgg aaaaatagca atgaccaaat caataataag tgggaggtaa
    39721 ttagaagatg agtgtgttca gtggaatgag aatatctgct agtggattaa cagcagaaag
    39781 tttgaaaatg gatatcatat catccaatgt agctaatata aatgctacaa gaggtccaaa
    39841 tggagaagca tataggagaa aagttgtttc atttgaagaa aactatgata ataaattagg
    39901 tatgctagga gtaaaagtta catctgttga agatgataaa tcacctctta aagaagtata
    39961 tgacccatct catccagatg caaagccaaa tggatatgta gaatatccaa atgtaaatat
    40021 attggtagaa atgacagatt taataacagc ttcacgagca tatgattcta acgtagatac
    40081 attgaatgct cagaagtcta tgatatcaaa agctttggaa ataggaaagt agggataaat
    40141 tatgccaatt acacaaatta acaatttaat gcaaggtgca aagttagata atttgattgg
    40201 agaagcaaag ccattaactc ttaataataa agaagaaaaa aagaattttg aagatgtaat
    40261 aatagatact atagataaag ttaattttca acaagtaaca gcagatgata tgaaagaaaa
    40321 atttataaaa ggcgaagatg tttctatgca tgaagttatg ttaataggtc aagaagcaca
    40381 actatctatg caatatttaa tagaagttag aaataagatt catgatgcat atcaagaaat
    40441 gaataaggtg caaatttagc cttttgaaca ggagtaataa tcaatgataa ccagttttaa
    40501 aaataagatt aaggagcaag ttgctaaatc taggacaaag ataagttctt taaaaaaggg
    40561 aactaaaata gcattaggta taggggttat tgctatatgt ttagcgctag tatttacagc
    40621 aatatctgcg agcaaaaata agtatgcagt cttattttca gaactagatg ctcaagatgc
    40681 tgcagatata tcagcagagc tagagtctaa aaagatagat actaaggtaa aaggaagtac
    40741 gatttatgta cctagaacag aagttgataa gttaaggctg gagttagcgc caagtcttac
    40801 aggtggttct aaaggatttg cactaatgga tgatagttca tcaatagggc ttactgatga
    40861 agaatttcag attaagaaac aaagaatgat acaaggtgaa atagaaaaaa caataaagag
    40921 ttttccacaa gtggaagaca ctagagtaca tataacattt ggagagtcat cagtatttga
    40981 gaaggaggct gttccaggga gtgcagcagt ttatttgatg ctaaaaccag ggacgacttt
    41041 agaaaaaaac caaataaagt ctgttatggc acttatatca ggaagcgtta caaatatacc
    41101 agagaaaaat gttcaggtta ttgataacaa aatgaattta ttatctgaag gtattttgga
    41161 ggatgaaaat gttgatgaaa ataatcaacc aataaatagt ggcattagtg ttgataaaaa
    41221 taaggcatct gaacaagcat tgaataaaca actagaaaag tctattttag aaatgctaga
    41281 accgatattt ggtaagggaa aggtgaaagc tacagtaaat gctgatttaa gttttgatac
    41341 agctgaaaaa actgagataa aaattgaccc tgataaagtt gcaataaagg agacaaggtc
    41401 gaaaaattca tctaaatctg ctgcaaataa tgttcaaggt gtagatgcta acatgaataa
    41461 ccaaggaaat aacaatggta ctaatacaga agatagttta gatgagagtt ttgagtatga
    41521 aacaggtaag attgaaactc acactatagt tgcgcctgga gaacttaata gattgacagc
    41581 ttctgttgca atagatggaa aaatagctaa aggtgttcaa gctgatgtag aggatattgt
    41641 taataatgct ataggtatgg atggagcgag gggagatact cttagcgttg tttcaatggt
    41701 atttgaccca gaaggtaaga aagaggctaa acaagagctg gaagagatta aaaaagaagg
    41761 atttaaaaag aacataataa aagcagttat tggagtagta gcagtaatag cagttggagc
    41821 tattggatac ttaatatata agaaaagaag tgaacgagaa gatatggatt atgaagatga
    41881 agattatatt tctaaatctg attctgttga taggaaggat aatcctctat taggagatga
    41941 tccagaactg acattagagg aagctgttaa aatgtatgca gaagaaaagc cagaccaagt
    42001 aacagagata ataaagactt ggttaaatga gtaaggggga tagaaagtgt ccacggaaat
    42061 tacaaatgtt aatgcgtttg acttaagtga tataaatgaa agtgataaga atgagaaggt
    42121 agaaggtgag ggtttaaaaa aagctgctgt attattaatg acattaggcc caaatgtgtc
    42181 tagtgatata ataaaaagct tgcctgataa acaagttcaa aaaataggtt tggaaattgc
    42241 aaatatttct tcaatagctt caaaagaaag aagagaaatc ttaaaagagt ttgtagaaat
    42301 aaatagggca aaagattatg ttatagaagg tgggcttgaa tatgcaagag agttattaag
    42361 taatgcttta ggaaatgtta aagcaaacaa attacttgaa ggtatatcaa taaatacaag
    42421 tacaaagcct tttaaacata ttataaaact agatgtaaag aagatattag aaatacttag
    42481 gaatgaaagt gcacaaacta tagccgtttt acttgcacat atggaaccag aaaatggagc
    42541 agaagtttta tcatacttag attttgattt acaaaatgaa gtagctatga gaataagctc
    42601 aatgggttca atatctccag aagtaataaa ggtaattgaa agttcattag aagataaatt
    42661 acattcaata agtggtgatg attttaatga ttatggaggg gtgtctacct tagttcaaat
    42721 attgattaat tctgatagaa aaacagaaaa gaatattttg ggaactattg aagaacaaaa
    42781 cccggattta gcagctcaaa ttaaatctca tatatttgtc tttgaagata ttgtcaagtt
    42841 agatgatata agcatacaaa aagtgttaaa agaagtgact atgaaagatt tagtatatgc
    42901 cttaaaaggt gtagatgatg agatttcaga tgctatatat agaaatcaat cttctagagc
    42961 agcagatgca cttaaagaag aaatggaaat ggttggtgtt ataaaggtta cacaagttga
    43021 ggcttctcaa caaaagatag ctaatatcgt aagaaaactt gaaagagaag gtgccataac
    43081 tattgtaagg aattttggtg gtgactttgt tgaataataa gattattaaa agtaaaaaag
    43141 cattatataa aggagtgcta gatttaacca taatagataa agttagagtg ggagaatcaa
    43201 atgtaaataa agaaaatgat tgtagattta gggatgaggt aataaaagaa gctatagaat
    43261 ctgctgagat aaaaaagaat gagatattag aagaagcaaa aataaatgca caaaatatag
    43321 aaaagcaagc atatgaaaaa gggtatagtc aaggtcaaaa aaatggatat gaggatggat
    43381 ataaagagtc atatgatgaa tatacccaaa aagctaaaga tgaagcagat tttataaaaa
    43441 atcaggcaaa tttaatctta ttacaagcta atgagaagat gactgagtac ataaaagaaa
    43501 aaaataaaga gattgtaaga cttgctatta atatggccag ttctgtttta aaaaagcatt
    43561 tcgaagatga agaatctatg agtgatctgt taaagcaaat tattcaagat tatgaaggaa
    43621 gtagttcatt tatagtaaga tgtaattctt tctataaaga taatatagag aaagagtttg
    43681 aattattcaa agaggaaaca tcactaaaat caagtgtatt tgttattgca gatgatagta
    43741 tagataaagg caatgctatt atccaaaagg aaaatggaag aataatagtt ggtattgatt
    43801 gtgctatgga aaaactaaaa gaagaattgc ttggtgaata aagtatgtat aaaactgata
    43861 tatctataga ttttgacaaa ataaataaga aaatagaaaa tttagaactc actatatcag
    43921 aaggtagagt aaagaagata ataggactta ctgtagaagt tgaaggaata aaagcttttg
    43981 ttggggagtt atgcgttata tacaaccaag ttaataaacc agtgaattgt gaagtagtag
    44041 gttttaaaga taaagaagtc atattaatgg cgttgggaga attaacattg atagcacctg
    44101 gttgtaaagt tatttctaag gggatacctt taagtgttat gtgtagtgat aatttactag
    44161 gtaaagtact tgatggtctt ggaaatccta tagacaactc agatgctgtt ttaggagata
    44221 gatacaacct gaacaatgaa cctcctgatc caatgaagag aaaaaagata cgaaatataa
    44281 tggaaacagg tgtaagagca atagatgcat ttactacttg tggtgaagga cagagaatcg
    44341 gcatatttgc aggaagtgga gtaggtaaga gtacgactct aggtatgatt gctaggaatg
    44401 caaaagcaga tgtaaatgta attgctctta ttggagaaag aggaagagaa gtactagatt
    44461 ttattgataa agatttaggt gaagagggaa tgaaaaaatc tgttgttgtg tgtgcaacat
    44521 cagataaagc ccctctagtt aggttaaaag gtgctcttac tgctactgca atagcagaat
    44581 attttagaga ccaaggcaaa aaagtaatac ttatgatgga ttcagtaact agatttgcta
    44641 tggcacaaag agaagttggt gtagcaatag gagagccacc agctcaaaaa ggatatacac
    44701 catctgtatt tgcaatgttg ccaaaactta tggagagaac agggacatca gataaaggtt
    44761 ctataactgc attttataca gtattagttg atggagatga ctttaatgag ccaatagcag
    44821 atacaacaag agggatactt gatggtcata tagttttatc tagagacttg gcaaataaga
    44881 atcactatcc ttctatagat gtattaaaca gtttaagtag gcttatgaat gaaattgctt
    44941 caaaagaaga cataaaaata gcgtcttttg caagagacat gcttgctgag tatagagaag
    45001 cagaagattt gataaatata ggtgcatatg cttcaggtac aaataaaaaa atagatgaag
    45061 caatctatta tcatgagcat attattaatt ttttaaaaca agggataaat gaaaagagtt
    45121 cttttaatga aactataagt agtttgagaa gagtttttga ataaacaatt aaaaaaatat
    45181 ttgaataggt tgtgatggaa ttggataaaa actttaagtt tagattacag aaagttttag
    45241 acttaaaagt gaaagatgaa gaagagataa aaatggaatt tgctaagatt caacaaaaaa
    45301 aaatagatat agagactaat ttagaaaact tagaaagtaa ttatagcaaa tattctataa
    45361 gtaaaaataa cgatagtatt caaaatcaaa aaataactat taactattta ctagctttaa
    45421 acaattctat aatggattta tcagaagagt tggataagtc aacaaatgaa cttgaaaaag
    45481 ctagaaagca gcttataagc aaacagatag agagaaaatc attagaaaaa ttaaaagaaa
    45541 aaaagtatgg acagtattat aaagaagaaa atttaaaaga acaaaacact aatgatgagt
    45601 ttgcaagtat gagttattta aggaatagac aagttctata agaatagtag gaagtgagga
    45661 tattattgta tgaagataca agcagattct tataatatta ttaaagatat tggggataga
    45721 aaaagttcat ctaagaacaa aaatataaaa aataataaat ttgaagataa tctatataag
    45781 gcaacagacc ataaaaaagt taaagataga agggaagtaa gtaagcaaaa tattagctca
    45841 gataaaccgg aaaatagtga taatgagtgt catttagatg aagataaaag taatcactta
    45901 gaagatgaaa atacatatga aaaagattgt agtaagaata atgaagcttt gcagcaatta
    45961 ctgaatcttt taaaggataa ttataaaaaa gataaagata taaacactat attgaataat
    46021 atagaaaaac taagtatatc agatgaatta aaacaagaac ttaagcttaa taaaaatata
    46081 ttaaataaga ttgcaaatca aaattcagag ctaaataaag ctaatataaa taactctaat
    46141 gatatagaaa atttagacat agttaaatta agagaacttt tggaagataa tgctaataaa
    46201 gatattgaag taattactaa aactacagaa aatgatacca aaaatataga tttgtatagt
    46261 tttgatagca atagaatgga taatcttaac tcaaataaac agaaagatga tagctctgat
    46321 attttggaaa aactagcagg ggtaaatgga aataagagtg ggaattttgg acaagtaata
    46381 aataaaaatg ttgatatgaa taaaattaaa aatgataatg tcaatttaaa acagatagaa
    46441 tcaaatgtaa tggatgactc tataaaagct attaagcata tgaaaacaaa tgatatacaa
    46501 gaattgacaa ttaaattaag accaaaagaa ttaggagata tgaatataca attgttaaaa
    46561 gatggtgaaa gtatgagagc tgttgtgact gttttcaata aagatgtctt tgactctata
    46621 aataaaaaca tagcagattt aaagcaacat cttgaactta ctaatgtaaa tataaaagat
    46681 gtatcagtgc aaatgcacag cgataataca aatacatctg atacctttga taaagcattt
    46741 gaacaacaga atagacaaaa taaccaagaa aacatgagta agaagaatac aaatgataag
    46801 cataataatg tagtcataga agatgacatt aaagaaaata ctattgatga tgatagagta
    46861 gatttactag cttagtaagg gggaagtatt gcatgtacgg aatagcaacg gctttggcag
    46921 gaactcaggg aaactatagg tcaaataact taagccaact tgaaagaaat attaatgata
    46981 ttgccaataa acaagttaga agcaaaggaa atcagacagt aacagacaat gggactccaa
    47041 ttataggaca aggtgatgat gctgataaag acttgtttat gaagatactt gtggcacaga
    47101 tgggtaatca agacccaatg aatccgcaag acccaacaga atatgtgtca caattagcac
    47161 agtttgcaac tttagaaaag atgtcagcta tgaatgagaa gctagatgta cttttagcct
    47221 taagtaattc cactctaata aactctgctt taagtatggc aacatcatta attggtaaag
    47281 aagtagaagt atctggggaa gagaaagaag atgaaaacat tgtaggagta gtaaagtcca
    47341 catatataaa agatggggaa gtacacttag aattagaagt agatggaagt gacaaaactg
    47401 taggtgttaa atatgaaaaa ttacttaaaa taggagaggt agatgaaaat tcaggagaag
    47461 aaaatgattc ttcagaacac gaatctccag aagaagcata atattaaatg agaaaagagg
    47521 taaattcatg ataaaagcaa tgtattcagg tgtaagtgga atgaaagcaa atcaaacaaa
    47581 attagatgta ataggtaaca atgtagccaa cgctggaact acttctttta agaaaagttc
    47641 agctagaata acagattcat tttatcaaac tgtactatat gcaagtgctc caacagctgc
    47701 attaggagga acaaacttag gacaagtagg tgtaggtagt aagatatcaa gtataaataa
    47761 agatatggta caaggaaatg tacaaccaac taatagacca tctgatttaa tgatagatgg
    47821 agatggatat cttccagtag taagacaagg aacagttatg tatacgagag atggttcttt
    47881 taatttagat acaggattta aagcagctga tggtgctttg aagaatatta aaggtggaag
    47941 acttgtaaat ggtgatggat atatgttaca aggtatgatt tatgatgggg aatataaagc
    48001 tgatactgga gaatttgtta aaaagggtga tgctaaagga aaagagccta ttgtaatacc
    48061 acttcaaagt ataagtccta aggatggaac agttcaaaat gtactagagt ataatatatc
    48121 aaaggatgga acagtagaat ttttattgag tgatggtcaa agaactcaat ttgtctatga
    48181 tatacctgat ggtgctaagg taggagatac acctgctgga atagataaat taaaaggtac
    48241 aatacaaaaa gtgcaaacat atgcattcca aaatccagca ggactagatg cagaaggagg
    48301 aaacttgttt aaaccatcag caaactcagg agacccacaa atagcaggag ctacaggcac
    48361 aataatacaa ggggcaatag aagcttccaa tgtagatata gcagaagaat ttacagaaat
    48421 gataatagca agtaggtctt tccaagcaaa cagtaaaata atatcgacat cagacgagat
    48481 acttcaagaa ataataaatc ttaagagata gtaggtacat attatgataa gagtaacgga
    48541 tttaaacgga agaaaataca tattaaattc agaccttata gtgaggattg attctgtacc
    48601 agaaagtagg attctcctta caacaggaga aaaaaacctt gtaaaagagt cggtagatga
    48661 gatagtagat aaagtaataa agtataaaag aaaaataaat gtaggttata ttaggagcga
    48721 aaaataatga aaaaaacaga ttcactgaca tttattggac taatagtatc tacagtatta
    48781 gttttagttg gtgccgcaaa aggaagtagt tcagggttaa agaatttttt tgatgtttca
    48841 tcaatattaa tcacagtgct tggttcattt ggagctctaa tgattacatt tactatagat
    48901 gatataaaac ttataaaaaa tgcattacaa tactcattta agacaatgag tgtatcaaag
    48961 ttagatttac tagaacaatt taaaacactt tctaaaaaag ctagaaaaga aggtctttta
    49021 tcaatagaga gtgatgtaat gggaatagaa gatccattta tgaagaaagg gttagagctg
    49081 tgtattgatg gattggaaat caatgagata aggtccatct tagaactaga gatagattct
    49141 gttgaagata caaataacag atactctaaa ctatttaaaa cttggggaac ctttgcacct
    49201 gcttttggga tgttgggaac actaataggt ttaatacaaa tgttagctga ccttacatct
    49261 ccagatttaa tagcatctgg catgggaaag gcattaatta caacgtttta tggttcttta
    49321 cttgccaata tcgctttgaa cccaattgca tataatatag atgagaagac tgaaaaagaa
    49381 atttatgtaa aggaaatgat gttggaaggt ataataagca tacaatctgg ggagagcagt
    49441 atagtagttg aggaacgttt agccacatac ctttcaaaca atgagaagtt agaaattatg
    49501 aaatccaata aaaatacaga aagagcaatg agcaatggcg cgtaaaaaaa ataagaaaaa
    49561 ggatgagata aatccagatg catggctggc tacttatgca gatactataa cactgattct
    49621 tacatttttc gtattattgt atgcaacatc agttgtagat atggaaaaat ttaaaaacat
    49681 agcagatgcg ttaaaaggtc agttttctgg gacaagtata tttagtacgg gttcaagtac
    49741 aagtgagaaa tctccaatag acagtgtttt agtggataaa aatgataaat caaatttaga
    49801 acaggattta aaagataaag ttaataattc taacttacaa aatagtgtaa cagtcaaaga
    49861 tgatagtaga gggattttac tagagttaga tgattcaatt ttatttgact ctgggatttc
    49921 tgagttaaaa gtagatagta aagctactct taataaagta tatgacatga taaagatgat
    49981 gaaaaataag atagttatag aaggccatac tgataatgta cctgtaaaat caagtacaca
    50041 tgaatccaat tgggatttat cttctagtag agcagtaagt gttgtaagat actttgtaga
    50101 agaaaagggc atggacccta gactattttc ggcaacagga tgtggagagt atcaaccttt
    50161 ggtttcaaat gaaacagcaa agggaagggc tgagaataga agagttaata tacttataac
    50221 tattgactag gaggaagagg tttgttcaaa aataaaaaaa ttattataat tattttgata
    50281 ctgattattt tctcagcggg agcaggtttt tttatattag gagaaggtgg aaataaacaa
    50341 gttgacaaga ttgcagattt gtttattcca acaggaaaag gtaaagatga agtaaagacc
    50401 gaaaatcttg acttagaaga gatggtatta aagctagctg atgaaggtgt aagatattta
    50461 aaagtatcaa taactgtatc ttatgattct tcatataaag atatagaaaa aaatacgcct
    50521 ttaattaggg ataatattat aaattgtttt atggcaaaaa gggcagatga ttttactgct
    50581 gataatttaa agaacataaa agaagaaata aaaaatacat taaatataca actaggtgaa
    50641 aatgtaattc aaaatatata tttcacaaat atagtagtgc aataaaagga tggtaagaca
    50701 tgatacttta tttactaaaa atattaatat gtatcccaat aattttaata ttgatagtct
    50761 tatctttaaa aattagccaa ggaactttat taaaaaaata taatgataaa tatgtaaaaa
    50821 tactagatat tgtaaatata agtaaaaaca atagcataat aattttaaaa ataggtgaag
    50881 aaggatgcat tatggcatct tcttcttcta gcatgagtaa aataaaagat ttgaccaaag
    50941 aagaaatatt aaaaatagaa aacgacatta aagaaagaaa ttttaaaatt caagatttta
    51001 aaatggaaag aattaaaaag aaatatgatt tcagtagata tattggaaaa tttacaaaaa
    51061 aggaagataa gtaaatggaa ctattatcaa aattaccttt ggctgatgta ccatatacaa
    51121 gttcaatgca attgttttta tttctaactg cattaatgtt tttaccattt gtaatgttca
    51181 tgatgacaag ctttgtcaga atagttataa gtttatcatt tttgaaatca gcattaggag
    51241 cacagcaagc aattccaagt caaattttgg taggacttgc aatagtattg acaatattta
    51301 taatgagacc agtattaaat gagataaacg aaaaggcatt acaaccatat atgaaagaag
    51361 aagttactat ggaagaagct atgaaggaag cagaaggacc tattaaagag tttttattaa
    51421 cacaaaccag acagacagat ttagatttat ttgtagaaca ggctggttta aaagaaaaga
    51481 aattaactcg tgaaaatata ccactatctg ttgtagttcc agcatttgct ataagcgaac
    51541 taaaaactgc ttttcaaata ggttttttaa tatatatacc atttttgata atagaccttg
    51601 ttgtggcaag tgttttgatg tctatgggta tgtttatgtt gcctccagtc atgatatcat
    51661 taccatttaa gttattatta tttgtaatgg tggatggatg gaacttgata gtaaaaacat
    51721 tgatattggg atttggatag gagaaaaata gtatgagtga atttatgata atgagtgttc
    51781 ttaaaaatgg catgtttaca ggagctatga ttgcagctcc agtactcatt gtttctcttg
    51841 tagttgggct tttaataagt gtgtttcaag ctacaactca aatccaggag caaactttga
    51901 cttttgtacc aaaactaata gctattcttt tagtagtagc tattggtgga agctggatgt
    51961 tacatactct tattagtttt acttctgaga tattcaatat gatatctctt ataactaaat
    52021 aagtaggata catatgattt ttgtatttat tagaatagta gcattctttg gaacactaag
    52081 aatactattt ccagctggta cacctgcttt atttaagtca ctctttgcaa tcattatatc
    52141 aatattgatt tcaagtacta tgcaaattga atatgcaaca aatatagaca acattgtatt
    52201 gtttacctta tatggtgtaa atgagaccat aactgggatt atgcttggat atataaccaa
    52261 cctatgtttt tatagtatca gaatggcggg ttctatgatg gaccaacaga tgggattatc
    52321 aatgattaat atgtttgacc caaattcaat gactcagaca actttaattg ataatcttat
    52381 gaactggaca gcacttatga tattttttag catggatggt catcatgttt taattagagg
    52441 tataaggtat agttttgagt taattccaat aggaaaacca tttgtagata ataacataga
    52501 ttatataata aacatatttg tgcagtgctt tttaactgga ttcaaaatag caatacctat
    52561 tgtactttgt ctgcttatgg cagattttat tttagggctt atatcaagaa gtgtacctca
    52621 attaaatgta atgatagttg gtatgccatt aaaaatattg gttggtattg cattatttat
    52681 catatcaata cctttaattg tcaatcaaat aagtcattta ttaagtcaaa taccaaagat
    52741 gtatgaggga acttttgcac tagctcctat gttttttatg ggttcagcag ataaaacaga
    52801 agaagctact ccaaagaaaa agggagaaca aagaaagaaa ggtaatatag caaagagtag
    52861 agaattaccc gttgcaatga ctctacttgc attcacctta ttagtaccta cattattttc
    52921 atatgtagta gatacactta aatcttcgct taattatttt ttgagtctag atttttatat
    52981 gaacataaat tattcaaacc tagagaaatt agttatagca ggattaatgg atttttttaa
    53041 gatattttta ccaatagcta ttcccttttt agttttaggc ataatagcaa acctatttca
    53101 ggtgggaata ctgtttactg gtgaaacttt aaaaccaaat ttatcaaaat taaatccaat
    53161 tagtggattc aaaaacatgt tttctatgag aagtttgtca accttaataa aggatacagc
    53221 gattatatct attttggctt acataggata tacattcttc caagataatt atttagatat
    53281 attaaagcta ggtaacatat acctaccaac tttaatgtac acagttaaag acttagtata
    53341 ttcaatattg agtaagattt gtgtagctat gatagctata gcagttgcag attatgttta
    53401 tcaaagatac agtcataaaa agcaactgag aatgacaaaa caagaagtta aagatgaata
    53461 taaaaactca gaaggagacc cagaagtaaa agctaagata aaacaaaaac aaagacaaat
    53521 ttcgtctcaa aggacaatgc aagcagtacc aagtgctact gtaatagtaa ccaatccaac
    53581 ccacttatct atagcagtga gatatgaaaa agggaaagac caagctccag tagtggtggc
    53641 aaaaggtgca gattatctag cttttaaaat aagggaaatt gcaaagggaa ataacatccc
    53701 tataatagaa aacaaaccta ttgctaggtt gttatataag caagtagaaa tagaccaaga
    53761 aattccagag gacatgtatc aagcttttgc agagatatta gtggcagtat ataaaatcaa
    53821 aaatagatac aaagtcccca aaaggtagga taagtaatga ataagtttaa tttaaaggaa
    53881 aatatggatg taatagtggc atttggtgtt gtagggattg ttcttatgat gatagtgcca
    53941 ttacctacat ttatattaga tattttactg gcaattaaca taagtttatc tgtgctaatt
    54001 ttactaatga cattatttac aacaaatgta cttgatttat ctatatttcc aacagtgtta
    54061 cttgtgacaa cactatttag attgggatta aatctttcat caactagatt gattttaaca
    54121 cagggatatg caggtgaagt tatagaggca tttggaagtt ttgttgtagg tggaaattac
    54181 gtagtaggaa taattatatt cttaattata ttaattgttc aatttgttgt tataaccaat
    54241 ggttctggaa gagtatctga agtaacagct agatttacgt tagatgccat gccaggtaag
    54301 cagatgagta tagatgcaga tttaaatgca ggagttatag atgacaaaac tgctagaaaa
    54361 agaagaaaag agcttcaaca agaagcagat ttttatggtt ctatggatgg agcaaataag
    54421 tttgtaaagg gagatgccat agcaggtctt atagtaacag caataaacat aataggtgga
    54481 attgtaatag gagctgtaat gctgaatatg actcttatgg gtgcagcaca gacatatgta
    54541 agacttacta taggggatgg tcttgtaagt cagatacctg ctctattaat atctacatca
    54601 gcaggtataa tcgtaacaag agcttctact gatgaaaact ttggtgactt agtaggtaaa
    54661 cagttaacgg caataccaaa agcagtaatg atgactggtt gtgttctaat cgtacttggt
    54721 atgatgcctg gattacctaa accagcattt ttttcactag gtataggagc tatagcagca
    54781 ggttactttt taggaagaga aaagaaagat actttagaag acttggagtt tgaaaaacct
    54841 gatatggtaa acccagataa aaatgaagta gaagatgtaa caaatttaat aaatgtagaa
    54901 tcaatagaag tagaaattgg atatggttta atatccttag ctgatgaatc agcaggagga
    54961 gatttacttc agagaatagc atcaataaga agacaatgtg caatagatat gggtatagtc
    55021 gtacaaccca taagaataag agataactta cagttaaatc caaaccaata caatataaaa
    55081 ataaaaggaa atgtagttgg tgggtatgag ataatgccta atatggtttt atgtatggac
    55141 cctatgaatc aaggatttag tatatttgga ataaagacaa ttgaacctac ttttggaata
    55201 gaggcacttt ggatagaaag tgacaaggta gaagatgcag agttaaaggg ttatacagtt
    55261 gtagattcat ccacaatact tataactcac ttactagata taataaaatc aaatgcacac
    55321 gaattactgg gaagacaaga agttaagact attatagatg cagcaagaga aaattatagt
    55381 gctgtagtag atgaattgat accagatttg atgacacttg gtgaaattca aaaagtactt
    55441 aaaaatctgt taagagaaaa agtaaatata aaggatagag ttacaatact tgaaacgctt
    55501 gcagaccagt ctagaaatac aaaagatata gagcttctta ctgaatatgt aagaatagcc
    55561 ttggctagaa gtatttgtaa taatttagtt gatgatgata agacaattgt agtggctaca
    55621 ttgtctccag aaactgaaga gttggtatct aataatttac agaggtctgt aaatggaact
    55681 tatccagcag ttgacccaga aaatacaaat aaaatatttg aaagtataca agaagtaatg
    55741 agcaatgtat atttcaatca taatatacca gtaatagtag tatcaccaaa gataagagct
    55801 ccatttagaa aattagttga aattgtatat cctaatttga ctgtattatc attaaatgaa
    55861 atacctaacg atattaaaat caaagcagaa ggtgtggtga gcatatatga tgacgaaaaa
    55921 gtatacagct aacacaattc aggatgctat gaatctagcc aaaatggaat tgggtgacaa
    55981 tattacatta atagataaaa aagaggtaag aaagtctgga atacaaggta tcttttcaaa
    56041 aaaagatatt gaactaacta ttggatggga aaagagagaa aatgtagaac aaaaagattt
    56101 aaaacgagag atagaacaat taaagtcaat tattaacaat atggggtttg ataataagaa
    56161 tgataatgat atagataaaa tatgtaaaaa tctactaagt ttagaattga atgaagaaat
    56221 tgtagaatct ataagaattg atttgcaaga aatgaagttc aatggaatcg atacaagtaa
    56281 aaatttagta gaaattttaa agaaaaagat taaaatagaa aatcaagtaa taaatggtaa
    56341 aattgctcta gtcggaccac caggagtagg taaaacgact acaatagcaa agttagccgc
    56401 aaaattagtt tttgaagaaa ataaaaaagt aggagtaata actatagata catacaggat
    56461 tggtgctgta gaacagttga agatatatac tgatataatg aatattccct ttaaaggggt
    56521 aataagtcct gatgaaatgg aattggcact tgacgagatg aaagattgtg atgttgtatt
    56581 aatagataca acaggtagag gctacaaaaa ctctatgcaa atactagaaa tcaaaaatct
    56641 tatagataaa gctgaaactg ataatataca tttagttttg aattgtacaa caagagaaag
    56701 tgatacaaaa gcaattatag attcatatag aaatgtgaat tttaaaagct taataattac
    56761 aaagctagat gaaacaataa catatggctc aatatttaac ataatgaatt atgcgcaaaa
    56821 accaatatca tatattacaa caggacaaaa tgttcctgat gatattataa aacctaatga
    56881 agataagatt ataaggttac tattaggagt tgaaagtata tgatagacca agctcaaatt
    56941 ttgagaaaaa tagcaatgga aaaaagagga cttgatgagt ttatagtaga gaatgagaat
    57001 gtacctaaga taattacaat agcttctggt aaaggcggtg ttggaaagag caatctcgct
    57061 acaaatttgt ctatatgctt gaccaaactt aataaaaaag ttcttatatt agatgcagac
    57121 ataggaatgt ctaatataga tataataatg ggggtaaatg taaaagggac tataattgat
    57181 gtaattaatg gcgaaaaaaa tatagaagat ataatttcac agactaagta tggtgtaaat
    57241 ataatatctg gaggttctgc ccttaatcat atagaagact ttacagaagc tcaaagaaat
    57301 aagtttatac atagcataga acaaattcac gatgtggatt ttataataat tgatacagga
    57361 gctggtatga gcaaaagcct attgtcattt atatactgta gtactgaatt tttcttaata
    57421 acaactccag aaccgacatc attgactgac gcatatagtt tattaaaagc tataagtaat
    57481 tttggcataa agagaagtgc aaatataata atcaataggg taattgattt agaagaagca
    57541 aaatctacat acaaaagaat aaaaatggtt gtggacaaat ttctaaacct taatctagaa
    57601 ttgtatggat atataataga tgacaaaaaa gtaagtttat gtgtaaaaaa acagatacca
    57661 ttcattttag aatatcctac aagtattgca tcaaaatgta ttatagccat agcaaagaga
    57721 atggtaagtc agtcaagaga agatattgtt gataataaaa gttttctaag aaagatgttt
    57781 agtatattta aaagctaagg aggcgtagtt aatgaataga gaagaattaa taaaagagaa
    57841 tatgcctctt gtaaagagta tagcaagaaa attttttata ccagggaaag gttttgaata
    57901 tcaagactta gtaaatagtg gagtcattgg attgattgat gcaataaata aatttgatgc
    57961 tgaaaaaggg gcaaagtttt ctagttactc ttatataaaa ataaaatctg ctatattaga
    58021 tgaaattaga aatcaaagtc caatttctaa acataatcta actaaagtaa ataagtataa
    58081 tagagtggta gaaaagttgc aatctaaatt attaagagag ccaacttcat atgaaattgc
    58141 aaaagaatta aaggtttcag aaaaagaact tcatgatatc gaaagtaata tagatatgtt
    58201 aaatattgta tcattaaact atgtaatctt tgaagataca aatgaaactg tacaagatgt
    58261 aataagtgat agagaagagg aagctccaga aaatataata gaagaagaag aaaagttaga
    58321 gattctatcc aaagcaatta gtaatttaaa tgaaagagaa aaattaatac tttcactgta
    58381 ctattatgaa gatttaaatc taaaggagat agggaaagtg ctaggtgttt cagaatcaag
    58441 agtttcacaa ctccacagaa aatcaataag aaacttaaga aacaagataa aagaacttaa
    58501 atattctata tagatggtga ttgataagat gatacaaatt atattaatag tagtgtctat
    58561 tttaataata gtaactatag ttataaattt acataaggaa tcaaaaaata aagataacag
    58621 atactttgat aaaatatttg agcaagaata tgaagcaaat gatagattaa taattgcaga
    58681 aaaaagaaga ttctttttag acaaagtact tgcagaaaaa agaattaata gtcatataaa
    58741 agtttatgaa aattataacc taataaaggc taatgaagaa agaaaggaaa acatatttat
    58801 agaatcaaaa aaggaagtaa gacaagaaaa tgagttatct cttaaagtga aacatcttat
    58861 aagttcaggt tatgatgatg tggaaatatg taagatgcta gatattggaa agggggaact
    58921 gttattaata aggagttgtt acaaaatata aggatattat tatgtgataa aaaagtattc
    58981 ttaggaatag gtattggtat attaatatca actttatgta taatgctttt tagtaataat
    59041 gatatgagta aggcagagat agaaaaaaag gccagagcgt taggcatgaa atatacaagt
    59101 gagatgaaag ttttagaata gatgatattg tttagacatt tacaaaaaag gaggtcataa
    59161 tgtgataaga ggattatata cagcagtttc tgctatgata actaatcaac aaaagcaaaa
    59221 tgttgttgtc aataacctag ctaatatgga tacaaatgga tataaaagta aacaactttt
    59281 gactaaaagt tttgatgaat taaagttgga gggttacaat aactacgcaa atggaactaa
    59341 acaaaagcag attataggcg atataagtcc aggtatttct atggatgaaa ctatcacaaa
    59401 ttttaatcaa ggacctataa aaactactga taataaaatg gatgtagcta ttcaaggtaa
    59461 gggatttttt ctagttagtg atgcagctgg aaatcaattt tacactagaa atgggaactt
    59521 cagaacagac aatcaaggtg accttataac aagtgaaggg tattatgtac ttgggacgaa
    59581 tactgctact ggtgctgtag gtcatataaa tgtaggaaat caaaaatttg aagtggcaaa
    59641 agataataca atatcactag acggaaatgc tatgtataaa tttaatattg tagattttaa
    59701 taactacaat aacttaaaaa aagaaggaga taacttatat tctggaggag gagctatgaa
    59761 agtaaataat gcacttacaa tacaaagtgc gatagagtcc tctaatgtag atatggtatc
    59821 tgaaattaat aatatgatga caatatcaag agaatatgag gcaaatcaaa agataattca
    59881 ggctatggat tctaaattag caaaaatagc aagtgagatt ggttcagtaa gataggtaat
    59941 aggaggtgaa tttttatgta cacaatgttt tatactagta aaacagcttt atcagcaaat
    60001 caaagtaaac tagatataat atctaacaat atagcaaatt ctgattcaac aggatataaa
    60061 aaaataaata tggaatattc tgatttagtg gatgaagtac taaatagacc atcatatcct
    60121 acaaatggca atgatatatc gactggaaca ggagctaagg caacagctcc catgagaaat
    60181 tatgaacaag gtgcattggc tcctacagat agtaagagta atttggcaat agatggagaa
    60241 ggattcttta gagtgataag aaaagatggt acatatgcat atacaagaaa tggtggattt
    60301 aatgtagatg ctttaggtaa gattgtagat gataatggaa atatattaga tgtccaattt
    60361 gatgcaggat ataactataa taatacaaat atagatagta caaacctgac agtttcaaga
    60421 aatggagagc tattcgtaga taataaaaaa ataggaaaaa tcaacttata tcaacctata
    60481 ggaactcaaa attttatatc tgaaggagat agtttattcg tcgcaactga tgcagcacag
    60541 attaaacaag ttgagaaagt cgatatagtt caaggctata gagagaggtc aaatgtttct
    60601 ttacaagaag aatttgttga cttaatagcc actcaaagag catttcaaat gaactcaaag
    60661 ggaataaaga cagcagatga aatgtggcaa atagcgaata atttaagagc taaataatta
    60721 tgtaaggtga ggaatattca tgaatgatat gaaagagtac gattttggca aacctcagag
    60781 ttattcaaga gaaaatttaa cttgtttaaa ttttttatta gctgaatttt gtaaaaaata
    60841 taaaaactat ataatttatg aacttaagtg taattctagt atggttgttg atagtataga
    60901 ccaagtaaat tatcagactt ttttagatag agtaacttct tcatgcgtag tagtacaaaa
    60961 tgcaatggaa cctgttatga aaaacttttc ttttagaata gatagaagtg tagctgatat
    61021 gtggattgat ataacttctg gaggaactgg agttgtaaag gataatgata gagaacttac
    61081 agaacttgat aaaaaagtgc ttttacacct tatggatgat ttagtaagaa atatgcactt
    61141 atttgagggt tttgaaaata tacaagtatt ggatacacat acacatatta atttgccaca
    61201 attatgttct ccaactgcac cagtatgcgt tgtagattta aaagtgctaa atgataatga
    61261 gtatattgga agtgtttctc tttgctttcc atacaatggt ttagaggctc tgttagaaag
    61321 tatatctgtt atagaatttt ttgataatga tgtaggagat gattcagagg aatttaccca
    61381 aaaaatctat aatagtgtat ctaatataga attatcagtt attgcagaga taggaaaagt
    61441 atcaattaat gttgaggatt tactcaaact agaagttgga gatgtaatag taacaaataa
    61501 aaaaataaat gattatatag atatatttgt agaaaattca aaatcataca cagcaacacc
    61561 aggatttata tcaagtaaaa aaggtgtaaa aataaatgat gctgttggaa aagaggtgta
    61621 gttatggaca atagtaatct tatttcagat gaagaagcaa agatgttatt aaatgataaa
    61681 gttgaaagtg aagttgatgg agatgatgag ttagccttag atgtctctga agttaatcga
    61741 aataatatac aaaggatact tgaccttaag ttagaattat ctgtagttat aggaagaact
    61801 aagatgactc taagagatat tttaaattta cataaaggtt ctcttattga actggatact
    61861 ttggcagacc aagatgtgga aatactaata gataataagg ttttggctta tggaaaagta
    61921 gtggtggtag atttaaattt tggtgtaaag ataacaagta tagtaagtga agaagacatg
    61981 gtaaaatctc ttgtttagag ataacatcaa ttaatactta aggcatgagg tggtacttat
    62041 tttaaactta aatgatataa aaaaagatat actaaaactc aataaaaaag actcatgttc
    62101 tctatataat gaatctatta ataatataaa taataattgt atatcaactg gattaaaatt
    62161 tttaaaacaa gcaagagatt taaacccaga tgatgttgat atattaaatc tcacaggtct
    62221 tgtaaactta ctaaaatgta attttgatga tgctgtagaa agtttctata agagttcact
    62281 ttgtgaaaaa actgctctgt gtgaaaagta tataaacatg cttacatctg aggagttttt
    62341 aattttcttt gaaaagtaca atcaacttat aaggttaata aataaaggaa tgtataaaga
    62401 agctatacaa gggtttaaac ttataactga ccaatactgt gatttaatag aaccttatga
    62461 gttgcttgcc ttactgtatg acaaggaaga agaatatata aaatttgatg aatgtttaga
    62521 agtattgaag attattgata aggaaaatta tttattaaat caagaaaata acacattata
    62581 tatgtataat agtgctaata tacaaactaa aagtaatata aaagtagatg attataagaa
    62641 tgagttctta cataaaagta gtagtaacca taaaattaat gattataatg atgtaactaa
    62701 agaaaagagt tcaagcaaaa accagaaaag ttcagattct aataagaaaa aaagtattaa
    62761 aaaaaataag tacctatata tagccataat attaggaata ttattagtag gacaaagtat
    62821 atatagttct tataagatga ataatttaac tgataaaata tctaaaaata atgaaactaa
    62881 aaatgaagag tcaatagatt ctaacttgac taagaaagaa aataatatag aagaaaatga
    62941 agatatgagt aaggcttcta atataaataa gagtaataaa aacattaata aagaggatga
    63001 tgtaaaatct aaaaaaaata ctgtagaaaa tgaagatgat aaagattctg acttatttac
    63061 agaggatgaa ttgatgtcta aggcgaatac tttaaaatca gaaaagaaaa ctaaatcttc
    63121 tatacaacta tataagaaag ttgcagatat aggtgaaaat aaagggaata catctgaagc
    63181 aacttatcaa gttgctattc tcagtgaaaa gttaaaagac tataaaactg ctgaagaata
    63241 ttataaaatg tatgtagaaa attactcaga aaaagatgct tattttgatg aatcttatta
    63301 taatttaggt atgatgtact ataacaatgg cgatttaaaa aattctaaat tgacattaaa
    63361 gaagttggtt aataaggtac caaatagtat gtataataat tcaaaagtaa aagaaatttt
    63421 aaaagaagaa tagttaaatt agataagaaa aataaattat tttattttaa aataatatga
    63481 ttataatata ttgacatacc attttaaaaa atttaaaata ttaattataa aatgaggagg
    63541 gataaaaaat ggaatttgaa aaagggagta tttcaattca tacagaaaat atttttccta
    63601 taattaaaaa atggttatac tctgataaag acatatttat aagagagctt ataagtaatg
    63661 gatgtgatgc agtaagtaaa cataaaagac ttgtatcact aggagaaata tcagaaaata
    63721 agtcatcaga ttataaaatt acagtatctg taaataaagg agaaggtaca cttaaattta
    63781 ttgacaatgg aattggtatg actgaagaag agataaaaaa atacataaat caagttgctt
    63841 tttcaggtgc tgaagatttc tttaataaat ataaggataa aatggaagag tcaaatgata
    63901 taataggtca ttttggatta ggattttatt ctgcatttat ggtgtctaaa aaggtacaaa
    63961 tagatacact ttcatataca gaaggagcga ctcctgtaag atggatatct gaaggtggta
    64021 cagaatacga aatatctgaa tcagatgcta gaaatgatag aggaactact ataacactat
    64081 ttattgatga tgatagtaaa gaatttttag atgaatttac tgtaagagga ataataaata
    64141 aatactgttc tttcttacca gtagaaatat atttagaaga tgttgaaaga ttagaaagag
    64201 aagctaaaga agcagaagaa aaagctaaaa aacaaaagga agatggaaaa gaagagattg
    64261 tagatgcaaa agtaattgag cctttaaacg acactaatcc tctatggtta aaatctccaa
    64321 aagactgtac tgatgaggag tataaagagt tttatagaaa ggtattcaat gtatttgatg
    64381 aacctttatt ttggatacat ttaaatgtag attatccttt caatttaaag ggtatacttt
    64441 acttccctaa attaaaaaat gagtttgaac tgactgaagg aaaagtaaaa ttatacaaca
    64501 atcaagtgtt tgtggctgac aatataaaag aagttatacc agaattttta cttttattaa
    64561 aaggagttat agactgccca gacttaccat taaatgtatc aagaagtttc ttacaaaatg
    64621 acagggatgt aagcaaaatt tctaaacata taattaaaaa agtagctgat aagttgaaat
    64681 ctctttgtaa aaatgaaaga gaagaataca ataagttttg ggatgatata caaatattta
    64741 ttaaatatgg ttgcttaaaa gatgaaagtt tctatgagaa ggtaaaagaa tgtatattat
    64801 tcaaaactat tgatgatgag tatataacat tacaagatta tttagaaaaa tgtaaggaca
    64861 aacatgaaaa taaggtattt tatgtaagtg ataaggaaca gcaatctcaa tacattaaac
    64921 tatttaaaga atacgactta agtgctgttg ttttaaactc atcaatagat actcatttta
    64981 tttcatttat ggaatataaa gagaatggag ttaagtttaa tagaatagat gcagaccttt
    65041 ctgatgtatt aaaagataaa aatgaaaata aagatagtga agaaaacaaa gaagagatag
    65101 ctaagataga aggtctgttt aaagaagctg tcggagaaag agtgaagaat tattctgtag
    65161 aaggattgaa aaatgaagac actccagcta tggtattagt atcagaacaa tctattagaa
    65221 tggcagaaat gcagtcaaga tttgctggaa tggatttagg aatgaatttt gaggaagaaa
    65281 aaactcttgt aataaatgag aacagtccaa taatcaaaaa attggtttct cttaaagatg
    65341 atgaggaaaa gaaagataag attacgctta tttgtaacca gatagcagat ttagctttac
    65401 tttctaataa agaattaaag ccagatgagt tagattcatt tgtacaaaga agtaataagc
    65461 ttatgagtct tttgatagaa ttataagatt gaatttgaat acattgaagc aaatttataa
    65521 aaagtattca atatcagtga taagaaagaa gtgtctcaaa atgaactttt cagttcatga
    65581 ggcgcttttt ttatgtgaaa ttaaatttat tttaatcatt tcaacaatga aaaatatatt
    65641 tatctctatt ttgagacaga ccattcttta tttttttatc tttggttaat tttaaatatt
    65701 taagtaggtt ctaataaaaa actctattta gttaaatata gttaacttga tatataataa
    65761 gataatacat ataattaagt aaaagattgt tttttagttt atgtttaaat acataattac
    65821 actttgttaa aaacataaaa ttattactaa agtagaaata ggaggtttta gaatgaattt
    65881 taattataat ttgccagtaa atatcttatt tgggagaggc agaattaatg atgtgggaaa
    65941 agaagtatct aaatatggca aaaaagtatt gatagtaaca ggtaaaaata gtacaaagaa
    66001 aactggatta cttgataaaa caatagattt actaaaagac tctaaaatag aatatgaagt
    66061 ttttgataga gtagaacaaa atcctcttat aactactata tattcaggag tagaaattat
    66121 aaaatcaact ggatgtgatt gtgttttagg tcttggtggt ggaagcatta tggatgctgc
    66181 caagagtata gctttttctt ataaaaaccc aggagattta aatgattata tatttggaat
    66241 tagacaaggt gaagaagctt tacctataat cttagttcca acaacatcag gtacaggtag
    66301 tgaaggaaat tgttttgcag tacttacaaa ttcagaaact aaagataaaa aatctataaa
    66361 gaagaatagt atgtatgcaa aagcatcaat aattgaccca gaacttatgg ttacaatgcc
    66421 aaaacatata attgcatcag ttggatttga tgcacttgcc cataatatgg aagcatatct
    66481 ttctaatgga agaaaccctc ttgcagatgt acaagctatt tatgggattg aacttatatc
    66541 agaaaaccta ataaaggtat ataatgatgt taatgattta gaagcatggg aaaaaataac
    66601 acttgcaagt actattggag gaatgacgat tggtacagcg ggtacagcct tgccacatgg
    66661 aatggagcat cctgtaagtg gtcttagaga tgtaatgcat ggaaaaggac ttgcagcttt
    66721 gactcctact attatagaaa gttcatggga tagtgatatt gaaagatttg gtaatatttc
    66781 aaaggtatta ggaggaactt gtgcagaaga ttgttcagat gcaattagaa atttccttaa
    66841 aaaaatagat ttagatataa aactaagtga tttaggtatt gaaaaatcag atatagattg
    66901 gttaactgat aattgtatga aaatatcagt tgcaaatatt aagagacatc caaagtattt
    66961 taataaggaa caaataaagg aaatgtacca taagtcttta taatttgact ttacatattt
    67021 agttttatat aaaaattata aattaataat aaaaaattat aagtaattaa tagtaaaaaa
    67081 attgtgtata gaagttatat atataataaa tttgtcttaa gcttttattt tactttctaa
    67141 taaataaagg agtttaaaaa agggaagatt aattaagtaa tattttataa aattcttcga
    67201 tttaaaaact ttaagtagag gtgaaataaa tgtttaatcc caaaagaata atattcgaaa
    67261 aaggaacttt agacactgat gtgggtaaaa aaatatacaa taaatttaga gataataaag
    67321 atgtagagat aattaatata tcatcaaata ggattaaaga acatattcca ggtgaaaatc
    67381 tatatgaaca atatagaagt gggaagcaga ctatggtagt tgggattaga aaaagtctga
    67441 agtttcaaac atgtaaacct tctgctaatt atcaactacc acttgtcagt ggatgtatgg
    67501 gaagatgtga gtattgttat ttgaacacac aattaggtga taaaccattt ataagggtgt
    67561 ttgtaaatgt agatgaaata cttgaacaga caaaaaaata tattgaagat agaaagccac
    67621 aggtaaccat atttgaaggg gcagctacat cagacccagt tccagtagag ccatatacta
    67681 attctttaaa gaaaacaata gagttttttg gaagagagga aaaaggaaga tttagatttg
    67741 ttactaaata cagtgatgtg gatagcttat tggatgctga acataataat catacagaaa
    67801 tacgatttag tatcaatact cctagaatta taaatgaaca tgagcattat acagcctctg
    67861 caaataaaag aatagagtca gctgtaaaac ttgctagggc tggttatcag attggtttca
    67921 taatagcacc tgtgttttta tatgaaggtt ggaaagaaga gtataaaaat ctgattataa
    67981 gtataaaaga agctttacca aaggattttg ataaaaaaat tatatttgaa gttatatctc
    68041 atagatatac aactaaagca aaaaatagaa tactagagat ttatccagat acactattgc
    68101 caatggaaaa tgaggataga aagtttaaat acggtcaatt tggatatggt aagtacgtgt
    68161 atgataaaga acaattagat gatatgaaag agttctttct taaggaaata aatggagtat
    68221 ttaaaaatag tttaataaaa tatataatat agcaaatctt acctaaagat ttgctatatt
    68281 ttatttttat acattttatg tttataataa agcaatgaga tttagcacta tcatataaga
    68341 aaattaaaaa gggcactatc tatcaagaac attttttcta gttttgatac tataaaggat
    68401 aagagtaaca aaaatatcag gaggagttat agtatgcatg catgggagtc tattcaaatt
    68461 acattggatt taatagaagc aaatttatca gaagaaatat caattaatga attagcaaat
    68521 aaagcaaatt tatcaccatt ttactatcaa agactattta aaagattggt aaataagact
    68581 gtaatggagt atataaagct tcgtcgtctt gcaagagctt ctgaatactt agcagaacat
    68641 aagaatcgta tacttgatgt agctttaaat tttggatttg ctagtcatga gacatttaca
    68701 cgttccttta aaaaagcata tggattaact ccagaaaaat atagagcaaa tccagtaaaa
    68761 ctcaatcact ttattaagcc agaattaatt ttaaattatg ctatggttga tgaaggtgtt
    68821 ccactgattg cagatgatat agtgattgaa gtcaatcgta agacattgag taatccaagg
    68881 acatttgttg gaatagaaat agaagtacct atttgccagt tggtgggcgg tgaaacaact
    68941 ggaattgcaa tagtagagga attatggatg aaacttggaa gtcaaagaca taatatacca
    69001 aatcaaattc caggtggaaa tgaatttgct gctctttata tgggaaatgc aaaagaagga
    69061 aattgtatgt atatggcagg tgtagaggta gagtcaggta catcagtaga aggatatagt
    69121 acttttgagt tgccagctaa agaatattta gtatgtggat ttgaggcaga aaactttaat
    69181 gagttagtta attcagctgt ttttaaagca gataaattta tggaaagatg gatgaagaaa
    69241 cataatttaa caactactga ttttgcaatt gagatgtatt accccactac acctgaagca
    69301 gcatatctgg aacattggat tgttccggtt cctattgaac aatagaaagg atgatgaata
    69361 tgaattggca agatgcgttt tttcaagata ctaaacctac atttgagcaa attaatgaat
    69421 ttattaataa tccattatgg gacaagttaa ataaaacttt ggtttctact tataatgtag
    69481 aacctaagct tgaatacagt aaatgttcaa tgcaaagagg ttggaatgta aaatataaaa
    69541 aaagaggtaa atcactatgt actttatatc cacaagaagg ttattttaaa gctttggtta
    69601 ttgtaagtga aagcaataga gtagaggttg atttatttat taatacttgt tgtgattata
    69661 tcaaaaaaat atataatgag gtaaactttt ttaatggaag taaatggcta atgattcaag
    69721 tagatagttt attggtatta aacgatatgc ttgaactaat taaatttaga gcttaacata
    69781 tagaacagga tgaatatata agtcatcctg tttttatatt caatcagata aattaatata
    69841 tagtctataa tatgaataat ttagtaaatt taaatttact ttatctttct acattctttg
    69901 caactaagtc cacatgtgtt tttttcttgc atattagtag caccccaatt agcaaattgt
    69961 tgtaaaatag gcacaatact tttaccttta cttgttaatg tatattcaac ttttggagga
    70021 atttcattat attgtttacg atgaacaata ccagtagact ctaaatcttt taatgattga
    70081 gctagcatag tatttgtaat gccatccaat tttcttttta tgctactata tctaattgga
    70141 ttttcaactg ctaacataca tataataggt aatttccact taccaccgac aatgtctaat
    70201 gcataacgga ttggacaggt taattcttca acgtttttag ttatattttt ttcagtattt
    70261 atactcattt taattactcc ttagttatag tagtatcttt tttatacttt atcagaaaaa
    70321 actgcatact tgttttttct gtattttata ttaaaattat atcaaaggat aatccttaat
    70381 acaagtattt aaaaggggtg taagaattat gaaagcatta gaatttttga acaaggcacg
    70441 agtttattat ttagcaacag ttgatgagaa caatcaagca catgtgcgtc ctttaggatt
    70501 tgttatggaa tacaatggta aacttacatt ttgcacatct aatcaaaagg atatgtttaa
    70561 gcaacttatt tttaatccaa aggttgaact ttgttgtata gaccaaaacc ttaatactct
    70621 acgtatttta ggtgaagcag tatttgttac atcaaaagaa acacaagcta aggcacttga
    70681 aataatgcca agtttaggtc agatgtactc tgtaggagat ggtaagtttg aaatattctc
    70741 tattgataag gcaaaagtta gttgttgttc aatgagtggt aaaaagattg atgtagagct
    70801 ttaattataa ttgaatcgac atccatagtg aaaattaaga aaacgttatt actgaaatgt
    70861 tgatagattt aatttaaatg caataaaaag atttttaaat aatgaatatg taaaaatttt
    70921 tagttattag atgtgtattt caataatga
//
LOCUS       DBGJFU010000004        68687 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.004, whole genome shotgun sequence.
ACCESSION   DBGJFU010000004 DBGJFU010000000
VERSION     DBGJFU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.004"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(130..324)
                     /locus_tag="ACOIAS_000266"
     CDS             complement(130..324)
                     /locus_tag="ACOIAS_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2699997.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACOIAS_000267"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACOIAS_000267"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGZ2699998.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACOIAS_000268"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACOIAS_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGZ2699999.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACOIAS_000269"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACOIAS_000269"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGZ2700000.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="ACOIAS_000270"
     CDS             complement(3870..4640)
                     /locus_tag="ACOIAS_000270"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HGZ2700001.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="ACOIAS_000271"
     CDS             complement(4659..5249)
                     /locus_tag="ACOIAS_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGZ2700002.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5433..6401)
                     /locus_tag="ACOIAS_000272"
     CDS             complement(5433..6401)
                     /locus_tag="ACOIAS_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HGZ2700003.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6394..7371)
                     /locus_tag="ACOIAS_000273"
     CDS             complement(6394..7371)
                     /locus_tag="ACOIAS_000273"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HGZ2700004.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7686..9656)
                     /gene="ftsH"
                     /locus_tag="ACOIAS_000274"
     CDS             complement(7686..9656)
                     /gene="ftsH"
                     /locus_tag="ACOIAS_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGZ2700005.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9690..11078)
                     /gene="tilS"
                     /locus_tag="ACOIAS_000275"
     CDS             complement(9690..11078)
                     /gene="tilS"
                     /locus_tag="ACOIAS_000275"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGZ2700006.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11261..11689)
                     /locus_tag="ACOIAS_000276"
     CDS             complement(11261..11689)
                     /locus_tag="ACOIAS_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HGZ2700007.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11706..12512)
                     /gene="murI"
                     /locus_tag="ACOIAS_000277"
     CDS             complement(11706..12512)
                     /gene="murI"
                     /locus_tag="ACOIAS_000277"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGZ2700008.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12714..13199)
                     /locus_tag="ACOIAS_000278"
     CDS             complement(12714..13199)
                     /locus_tag="ACOIAS_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HGZ2700009.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13250..13909)
                     /gene="spoIIR"
                     /locus_tag="ACOIAS_000279"
     CDS             complement(13250..13909)
                     /gene="spoIIR"
                     /locus_tag="ACOIAS_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HGZ2700010.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14054..14734)
                     /locus_tag="ACOIAS_000280"
     CDS             complement(14054..14734)
                     /locus_tag="ACOIAS_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2700011.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14718..15608)
                     /gene="ispE"
                     /locus_tag="ACOIAS_000281"
     CDS             complement(14718..15608)
                     /gene="ispE"
                     /locus_tag="ACOIAS_000281"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGZ2700012.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15726..17276)
                     /locus_tag="ACOIAS_000282"
     CDS             complement(15726..17276)
                     /locus_tag="ACOIAS_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HGZ2700013.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17370..17636)
                     /locus_tag="ACOIAS_000283"
     CDS             complement(17370..17636)
                     /locus_tag="ACOIAS_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HGZ2700014.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17784..18644)
                     /gene="yabG"
                     /locus_tag="ACOIAS_000284"
     CDS             complement(17784..18644)
                     /gene="yabG"
                     /locus_tag="ACOIAS_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HGZ2700015.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18728..19666)
                     /locus_tag="ACOIAS_000285"
     CDS             complement(18728..19666)
                     /locus_tag="ACOIAS_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HGZ2700016.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19776..19937)
                     /locus_tag="ACOIAS_000286"
     CDS             complement(19776..19937)
                     /locus_tag="ACOIAS_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700017.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19947..20444)
                     /locus_tag="ACOIAS_000287"
     CDS             complement(19947..20444)
                     /locus_tag="ACOIAS_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HGZ2700018.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20559..21269)
                     /locus_tag="ACOIAS_000288"
     CDS             complement(20559..21269)
                     /locus_tag="ACOIAS_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HGZ2700019.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21445..21975)
                     /locus_tag="ACOIAS_000289"
     CDS             complement(21445..21975)
                     /locus_tag="ACOIAS_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700020.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21992..22222)
                     /locus_tag="ACOIAS_000290"
     CDS             complement(21992..22222)
                     /locus_tag="ACOIAS_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700021.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22237..23631)
                     /locus_tag="ACOIAS_000291"
     CDS             complement(22237..23631)
                     /locus_tag="ACOIAS_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HGZ2700022.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23757..25010)
                     /locus_tag="ACOIAS_000292"
     CDS             complement(23757..25010)
                     /locus_tag="ACOIAS_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGZ2700023.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25061..26215)
                     /locus_tag="ACOIAS_000293"
     CDS             complement(25061..26215)
                     /locus_tag="ACOIAS_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2700024.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26775..27344)
                     /locus_tag="ACOIAS_000294"
     CDS             complement(26775..27344)
                     /locus_tag="ACOIAS_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2700025.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27547..27774
                     /locus_tag="ACOIAS_000295"
     CDS             27547..27774
                     /locus_tag="ACOIAS_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGZ2700026.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27993..28736)
                     /locus_tag="ACOIAS_000296"
     CDS             complement(27993..28736)
                     /locus_tag="ACOIAS_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HGZ2700027.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28904..29776
                     /locus_tag="ACOIAS_000297"
     CDS             28904..29776
                     /locus_tag="ACOIAS_000297"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2700028.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29776..30441
                     /locus_tag="ACOIAS_000298"
     CDS             29776..30441
                     /locus_tag="ACOIAS_000298"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGZ2700029.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30462..31319
                     /locus_tag="ACOIAS_000299"
     CDS             30462..31319
                     /locus_tag="ACOIAS_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2700030.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31475..33988)
                     /locus_tag="ACOIAS_000300"
     CDS             complement(31475..33988)
                     /locus_tag="ACOIAS_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2700031.1"
                     /translation="MKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLMNYMTNGAIIKY
                     GDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKPYLFIAGFSKEA
                     FDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKRMSENEKLGQSD
                     PYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVKTDSLSLFVTLK
                     NPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVLAIIMLGSIFLI
                     YNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIGVAIGIVSIGLV
                     ISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPARKAANTPVMECI
                     RQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLSLVLSIVLFIST
                     SALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGVYEGLYQKLMTY
                     SCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSLGLPAEEYTGQN
                     AKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNISFVKVVPPDSPP
                     GIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSVAEMEQIIESEG
                     VTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTISTNIKLRRREL
                     AMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVMVTDDIDFVIPW
                     FSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34021..34707)
                     /locus_tag="ACOIAS_000301"
     CDS             complement(34021..34707)
                     /locus_tag="ACOIAS_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700032.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34790..35779)
                     /locus_tag="ACOIAS_000302"
     CDS             complement(34790..35779)
                     /locus_tag="ACOIAS_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2700033.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35766..36452)
                     /locus_tag="ACOIAS_000303"
     CDS             complement(35766..36452)
                     /locus_tag="ACOIAS_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700034.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36449..36571)
                     /locus_tag="ACOIAS_000304"
     CDS             complement(36449..36571)
                     /locus_tag="ACOIAS_000304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700035.1"
                     /translation="MDYIRTKETTENCVMISKIVVYYCSAGIYKTNKIKEDESK"
     gene            complement(36952..40158)
                     /gene="carB"
                     /locus_tag="ACOIAS_000305"
     CDS             complement(36952..40158)
                     /gene="carB"
                     /locus_tag="ACOIAS_000305"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGZ2700036.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40351..41400)
                     /gene="carA"
                     /locus_tag="ACOIAS_000306"
     CDS             complement(40351..41400)
                     /gene="carA"
                     /locus_tag="ACOIAS_000306"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGZ2700037.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(41657..44863)
                     /gene="carB"
                     /locus_tag="ACOIAS_000307"
     CDS             complement(41657..44863)
                     /gene="carB"
                     /locus_tag="ACOIAS_000307"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGZ2700038.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(44916..45959)
                     /gene="carA"
                     /locus_tag="ACOIAS_000308"
     CDS             complement(44916..45959)
                     /gene="carA"
                     /locus_tag="ACOIAS_000308"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGZ2700039.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(45985..46701)
                     /gene="pyrF"
                     /locus_tag="ACOIAS_000309"
     CDS             complement(45985..46701)
                     /gene="pyrF"
                     /locus_tag="ACOIAS_000309"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGZ2700040.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(47077..47712)
                     /locus_tag="ACOIAS_000310"
     CDS             complement(47077..47712)
                     /locus_tag="ACOIAS_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HGZ2700041.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(47736..48650)
                     /locus_tag="ACOIAS_000311"
     CDS             complement(47736..48650)
                     /locus_tag="ACOIAS_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2700042.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            49251..50480
                     /locus_tag="ACOIAS_000312"
     CDS             49251..50480
                     /locus_tag="ACOIAS_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGZ2700043.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWNIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(50685..53066)
                     /locus_tag="ACOIAS_000313"
     CDS             complement(50685..53066)
                     /locus_tag="ACOIAS_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HGZ2700044.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(53038..53763)
                     /locus_tag="ACOIAS_000314"
     CDS             complement(53038..53763)
                     /locus_tag="ACOIAS_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HGZ2700045.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKYVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(53753..54208)
                     /locus_tag="ACOIAS_000315"
     CDS             complement(53753..54208)
                     /locus_tag="ACOIAS_000315"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGZ2700046.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(54490..55671)
                     /locus_tag="ACOIAS_000316"
     CDS             complement(54490..55671)
                     /locus_tag="ACOIAS_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2700047.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(55661..56353)
                     /locus_tag="ACOIAS_000317"
     CDS             complement(55661..56353)
                     /locus_tag="ACOIAS_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700048.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(56487..57203)
                     /locus_tag="ACOIAS_000318"
     CDS             complement(56487..57203)
                     /locus_tag="ACOIAS_000318"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGZ2700049.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            57328..58062
                     /locus_tag="ACOIAS_000319"
     CDS             57328..58062
                     /locus_tag="ACOIAS_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGZ2700050.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(58347..59102)
                     /locus_tag="ACOIAS_000320"
     CDS             complement(58347..59102)
                     /locus_tag="ACOIAS_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2700051.1"
                     /translation="MKKYNISENGTLIKSDTIESKSERIKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRQIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     NFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
     gene            complement(59224..60162)
                     /locus_tag="ACOIAS_000321"
     CDS             complement(59224..60162)
                     /locus_tag="ACOIAS_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGZ2700052.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(60265..60915)
                     /locus_tag="ACOIAS_000322"
     CDS             complement(60265..60915)
                     /locus_tag="ACOIAS_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2700053.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKTKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            complement(61065..61253)
                     /locus_tag="ACOIAS_000323"
     CDS             complement(61065..61253)
                     /locus_tag="ACOIAS_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGZ2700054.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(61360..62544)
                     /locus_tag="ACOIAS_000324"
     CDS             complement(61360..62544)
                     /locus_tag="ACOIAS_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2700055.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLSVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(62686..63603)
                     /locus_tag="ACOIAS_000325"
     CDS             complement(62686..63603)
                     /locus_tag="ACOIAS_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HGZ2700056.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(63625..64353)
                     /locus_tag="ACOIAS_000326"
     CDS             complement(63625..64353)
                     /locus_tag="ACOIAS_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700057.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(64549..64749)
                     /locus_tag="ACOIAS_000327"
     CDS             complement(64549..64749)
                     /locus_tag="ACOIAS_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700058.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(64874..65254)
                     /locus_tag="ACOIAS_000328"
     CDS             complement(64874..65254)
                     /locus_tag="ACOIAS_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2700059.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(65527..66885)
                     /locus_tag="ACOIAS_000329"
     CDS             complement(65527..66885)
                     /locus_tag="ACOIAS_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2700060.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGLLRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            67333..67785
                     /locus_tag="ACOIAS_000330"
     CDS             67333..67785
                     /locus_tag="ACOIAS_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HGZ2700061.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRIFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(67942..68559)
                     /locus_tag="ACOIAS_000331"
     CDS             complement(67942..68559)
                     /locus_tag="ACOIAS_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HGZ2700062.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
BASE COUNT        21608 a        12626 c         7443 g        27010 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt gtttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attctacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttggattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttcttaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat gatgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttaccc
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgattcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaact
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt attgctaaag
     3241 cacttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa tcattcatta
     3481 tctctgctgc tcttcccata aactctggtt ctgaaccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccttg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattctaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat agctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta acccacctgt agctataacc ttaacatcat cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcatg
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctatctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtactttata caaaagtttt caagagaatg cataacattc
     4441 ggaactacag atgatattat aacatcatca atatcactta tattcacatt atcataatca
     4501 aataggttac ttattaggat tccatattca tcagacgttt tttctctatc tgttttaatt
     4561 ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac cccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttac tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt tcgttttttt cttataacta tgagtcaagc
     5341 taccattgta ggatgcccgc tctaacaaga ataattttat caagatgagt tgccattgtc
     5401 aacaatttga caagaaaaat atgctaattt catcatattt ctcgtttttt tactatatct
     5461 ttatatgcca ttatattttc tacactatcc gcttttttta ctgcatttat tatacttttt
     5521 tccacaacct ctacagccaa tgaacctact aatgttatat ctgcttctat ttctcctgtt
     5581 gatattgtga atattgtatc tccatcatga ggtgtatgga taggaaaaat agacttagca
     5641 tatccatcat gtcccatttg agatatcttt ttacatccag ctttggttaa cttagcattt
     5701 gttgcaatta tcccaattgt agtattatct acactaaatc cacctttact ttcaccttgt
     5761 ttcattaatt tatatgtatc aattgtttct gtctttttat catttaaagc accagctata
     5821 accttaccat tttcataaac atctccaaga gcatttacag caattaatgc agatacaatt
     5881 agaccattat ctaattttat agaatagcta cctattccgc ctttcatagc atattcctga
     5941 cctcttatct ttccaacagt ggctccacaa cctgctccat aatttccttg atttaaaata
     6001 tcagtatttg caacttcaca agctctataa cccatcttta aatctggtct acatttaaag
     6061 ctccctactc ctaaatcaaa taaaactgct cctacaacta taggcacttt acctacacct
     6121 acatcaaatc caattccttt ttcttctaag tatctactta caccacaagt ggattcaaga
     6181 ccatatgcag aacctccagc caaaactact gcatgaactt tttgaaccat atttacaggg
     6241 tctaataaat cggtttctct agtacctgga gcagagcccc ttacatcaac accgcaagta
     6301 gctccttctt cacatattac tactgtgcac ccagtaagac catttctatc ctcaacctgc
     6361 ccaaccttta agccttctat atccaaaata ttattataca tatccttttt ctcctcttat
     6421 agaaacttca cctgaaataa tttcttttat acttccatca cttctttgaa ctaataaatt
     6481 tccctcttca tttatgtcta aacatttcac tagttctcta ttatctcctt ttaatacata
     6541 tatattttta tctataattg cagaataatc tctacatatt tttaaggttt tttctttgga
     6601 attatttaca gtataatcta aatataattt ttcaaattca gtcaatacat tcctaacaat
     6661 atctactctg gataacatat acccttcttt atacaatgat gtggctttgt cagctatttc
     6721 ttgttcaaaa tccattattt taacatttat tccaattcca agaacaacat aatttgttct
     6781 ttcaatttca gcagataact ctgttaatat accacatatt ttttttccat ttaaaataac
     6841 atcatttggc cattttatta atgtatttgc tcctagattg tttaaagctt tcgttacact
     6901 tgctcctgct attaaagtta taaatggggc tttgaatggt tccatatctg gttttaatat
     6961 aatactcatc cagattcctt catgaggttt tgaatcccat cttcttccca atcgtccttt
     7021 ccccatatct tgatgttcac ttaaaatcac agtcccatct acactattta tagctatcct
     7081 tttagcatgt tcatttgttg aatctatagt gtcaagatgt tcaatatttt ttccaataaa
     7141 ctctgtatct aatttatagt aaatattctg tgggcttaat aaatcagttg gattttccgc
     7201 taatcgatac ccttttttat ttacagattc tatattatat ccctcttctt ttaaggcttt
     7261 catatgtttc catatagctg cacgtgacac ccctaattgt tttgataatt cttctcctga
     7321 aataaattca gatttattat ttaatataat ctctattatt ttatctctca tatttcacct
     7381 ctaatatgac ataatttcat gacttatttt cttctaataa ataattatac attattaata
     7441 tacttttaat actattaatt ttaaatttcc tttttaatta ttataatggt tgttttatat
     7501 tttatattga tgatttatat aattttacat tttatttaaa taattttaaa ataataaaaa
     7561 aactgaaagt atcaattaaa ttaatacatg caaatataat ttgcttatta atttaatatt
     7621 atactttcag ttttagtatt taatccacat cagataataa tcttaaaatg accaaggtct
     7681 taaaactatt tatctttatc agatttatct tctaaatttt tatttatatc aataacattg
     7741 tttctttctt cttcatcttt ttctaatttt atagttaatt gttcttcaac ttctttagaa
     7801 gaattttcct ctgtaactgc attttcaatt tcattcagag gtaactcttt attaaatgct
     7861 tttacaaatt gttctgcatc taaagtttca tatataagta aagcttgtgc tacatactct
     7921 aatctatcca tattttcttg taatatactt cttgtcttat catatgcacc atcaactata
     7981 cgctttgttt cttggtctat ctcaaaagca acttcttcag aataatttct cttacttgaa
     8041 aaactattac ctaggaatac ttcatcatca ctgtcaaatg acattggacc aagtttagaa
     8101 ctcattccat actttgttac catacttctt gcagtagctg taactctttc taagtcattt
     8161 gaagcacctg tagatacatc cttaagtact agctcttctg ctactctacc accaagtaaa
     8221 actacaatat tttctttcat ttcatttttt gtagcataga acttatcttc aacaggaagt
     8281 tgcattgtaa accctccagc tcttcctctt ggaactattg taacttgatg aacaggactt
     8341 acttcttcaa gtacatgggc acaaactgca tgccctcctt catggtatgc tgtaagtctt
     8401 ctttctttct cacttattac tttcgatttt ttagccacac ctgctataac ctttgttata
     8461 gcttcttcta tagtttccat cttaattttc ttttctcttt ttctagctgt taatattgca
     8521 gcctcattca ttaagttctc tatatcagca ggagtaaatc ctggtgttct tctagccaag
     8581 acatccattt ttacatcatc acttaatggt ttatttcttg agtgaacttt aaatatagcc
     8641 tctctacctt ttacatctgg tgtaccaaca acaacttgtc tgtcaaatct tcctggtcta
     8701 agtaaagctg ggtcaagtat atctggtcta tttgtagctg ccatgattat tataccttga
     8761 tttaccccaa atccatccat ttcaactaga agttgattaa gagtttgctc tctttcatca
     8821 tgacctccac caagacctgc acctcttttt ctaccaactg catcaatttc atctatgaat
     8881 atgatagctg gagcactttt cttagcctgt tcaaataaat ctcttactct tgaagcacca
     8941 accccaacaa acatctcaac gaaatcagaa ccacttatac tgaaaaaggg aactcctgct
     9001 tctcctgcaa ctgctcttga tagatatgtt ttaccagttc caggaggtcc taccataagc
     9061 attccttttg gtattcttgc acccaattca atatatttct taggattctt taagaaatct
     9121 actacctcct gcaaatcttc tttttcctca tctagtccag ctacatcttt aaatgtaact
     9181 ctagtttttt catcatcttt atgaacctta gcttttgatt tgccaaaact cataaccttt
     9241 ccgcctccac cttgagactg attcataaac acgaaccaaa taatcaccat gaagaatatt
     9301 aacagtaatg taggcaacat ttcgacaaac caaggtgtag atggcttagc ttcaccacca
     9361 aaagttagtt tcccagcctt agcttggtct aacacctcat ctgcaagttt atttcccatc
     9421 acttctgttg gaacatatga cttaaacttt gtatttgtat cttttatagt accttctaca
     9481 gaagtttgat ttacaaagta taatcttgat atattctcat ctgttaattc tctatatact
     9541 tcagagaatt ttaaatcttt tattttttct gtaggcttac ctgagaattg aactatacct
     9601 actataatta taaaaacaag tagataaaaa cctgcccctt ttagcaactt attcaatata
     9661 gaagccctcc tttcatacat tgctttgccc tataatttgt taaaattaat ttttaatact
     9721 tcttttgtgt tttcatcaat tttataattt tcactgattt tataatctcc aacacatatt
     9781 attccttcac tatctgtgat tataggaatt ttacatcttt cttctcttgg gatttttaaa
     9841 tctatgaata aatctttcac ttttttactt cccatagcaa gctttatctt atcaccttgc
     9901 cttctgcttc ttattactat accccctttt accttattaa aatcaaaccc tttagatgaa
     9961 ttgtctaatt tcatactctt atacctatct atgctcatta cttgggtctc aataatttgt
    10021 tttaattctt ttattttaat aaacccatta ctaggtacat tgtaataaaa ttcaatttcc
    10081 tcattaacaa tttcctcatt cgttaatatt atactgtctt tttttctata aacaaatata
    10141 tttcttggaa gagttaacat tttatttact tttgaattat cttctaaaga cattatatct
    10201 tctatatgtc tttggtcaac aaaattagta tctcctagta tatgttttat ggaatttcta
    10261 agaactctaa ctttgatagc atcatgtaag caaacaaaat cgtctaaatt tatctctaca
    10321 aagccttttt cttttatatt tgaaacttct ctaaatttag cttctgcctc tttctcaata
    10381 taatcgttat cactttttaa actattgctc atcctcacta tagattctat tacatttgaa
    10441 ttaaaattat ccttcatata tggtaaaagg tctaacctta tcttatttct agtgtatata
    10501 ttttctaaat ttgttttatc tatcctcgga tttaaattat aagcttcaca atactcctct
    10561 atctcatttc tttccacgtc taatatgggt ctaattatgc aattatctct aatataatca
    10621 attcccttta agccttttaa acctgttcct ctcatgatac gcatcataac cgtttcggct
    10681 tggtcattga gattatgacc tattgctatt ttattggctt taatcttatc cttaatttca
    10741 taaaacattt catatcttag cttccttgct ccttcttcta atgataatcc ctcattttca
    10801 caatattttg gtacattaat agactttaca aagaatataa ttcccatatc ctcacaaagt
    10861 ttagatacat ataatgcatc tttttgagct tctatccctc ttatttgatg gtttaaatgt
    10921 gctgcatata tttctatatt aaaatctttt tttaatctat ttagtacatg caataaacaa
    10981 actgaatcag gtcctccaga aagacctaaa actattttat cgcctttttg tattaaatta
    11041 tgtttattta tagtacttag taccttatca aatatcatat ttatccctct tacacttagt
    11101 tttattcact tttaagtata ctttctataa aaaaacgcta aacccttgta tcaattttaa
    11161 gaaattaaag tttaatataa atataataat accaaataaa ttaaataaaa ggtagttttt
    11221 tcactacctt tataaaattt ctggaaaata gccatagtta ttatttagta cctatgtata
    11281 tatctatttt attcctacct aaaaataaat cttgtacctt tatattatag tctattttca
    11341 attttatgtg ttccccttct tgtatattta tatctaattc tcttgtatca atatcaatta
    11401 ataaagtacc ttgtggggtc acatactttg tagtactact ttctccacat ttaaacagca
    11461 ttgttgagtt aatagcacca aactttttga tactaacttg atttttaagt atctttatag
    11521 tattagttat ttctatatct tcttcttttt ccttgtaaat aacatatatt ccatcatttt
    11581 tataaaaaat tttaccaaag gcagtcatct ctatggtgtc catatttcct ttttcatcat
    11641 attgtctagt atttatactt atttttacac tcaacgattt ttcctccata gattcttcat
    11701 ataaactagt atttttgtat ttctactttt tgaatcttat caattttttt ctttaagaac
    11761 tctcttgcta tatctgaaaa tttttcaggt atatctgata cataatattg ataagttcca
    11821 tgaatttctg tattgtttat aatattttgc acctctaaaa tcttttttaa atcctttgca
    11881 gtctctttag ctggattaac aagttttata tgctctccaa cttcttcacc tattgttctc
    11941 tttaaaatag gataatgagt acatcctaac actaaagaat ctatattttt ttctttcaat
    12001 tcttgtaaat atatctttgc tgtcagttta gccacttctg tatttgccca tccttcttca
    12061 acaataggca caaataaagg acatgcttta ttaacaattt ctatactctc atctatttta
    12121 gatatttcca agttgtatgc ttttgaactt atagttcctt cagttcctat tattcctaca
    12181 attttgtttt ttgtagaact tacagctgtt ctagctcctg cttctataac tccaattatc
    12241 ggtatatcat atttttcttg agcttctttt aagcttcttg cagtagcagt gttacatgct
    12301 ataactatag cttttacacc ttttgttttt aaaaagttta tagcttgaaa agtatattta
    12361 attatagttt ctttggatct tgaaccatat ggtattctgg cagtatctcc aaagtatatt
    12421 atatcctcat ttggtaatat cttcattatc tcttttagaa ctgttaatcc acctaatccc
    12481 gaatcaaaaa ctcctattgg tttattgttc atctttatac aacacctact ttacattttt
    12541 gtgtttaaca acaacttatt tatcgctaaa atttatattt atattcataa ttaatatatt
    12601 attacctaaa tgattattat agtttatttt acacaaaaaa taaaggagct atcttaaatt
    12661 agaaaataat ttctacaaaa agacaactcc tttcactact taaaaattta aatttatttt
    12721 gcaaacacat gatttcctat tctcatataa ggattcaatc tatttatcca tttgcaagtg
    12781 gctatttctg gattccaaaa atatactgct ttattagttg ggtcagttcc ataaagagct
    12841 tcctgagctg ctttacgtgc tgagtctgtt ggtgccatct ttattgaacc atcaagcaca
    12901 actgagaaag catttttttg atatatgact tcttctattg tgtctggaaa tctagggtcc
    12961 aatactctat ttattactac agctgctaca gcaacctgtc cctcatagct ttctcctcta
    13021 gcttctcctg caaccaactt agataataac atcaagtcat tatcagataa atttattact
    13081 tctttctttg ctatattttt ttcttgtttg tttactttct taatattttc tgttggttca
    13141 tatgcaaata cctctttaat aggcataaat ataaaagtca aagctaatag tgtagccatc
    13201 agtttactcc acctgtccat gattaaaacc actccttaca ttcttatttt taaaattttt
    13261 tctctaaatc cttgactact tcaagtactt taaatttaat ttttactcta cttgtttttt
    13321 ttggtgtatc ttgttttatt aacttatatt ctttatcagt taaaacttcc ttcaacttat
    13381 catctgtaga tttatctatc actccattag attcatcaac aaaacatagt ggagggaaca
    13441 taacacacca ccagtttttt ccttcacctt tcccaatgat aatctttaat gctttatatt
    13501 caccagctgg aagaactata ttagaatatt gtttagtagg gaaattgcta tattgtatac
    13561 ctacctttac actgtaatta tatccatttt taagaataat atttttactt atttcttcca
    13621 aactactata ttcctttttt ataatagctt cactttcttt tatacttttt gaattttgaa
    13681 gctttggctg taaatatttt attacttcat ctctaacttt tagtttaagt tcttggtctt
    13741 catctgtatt gctgtttgct ataacatgaa atcttattaa tttatcttta taatcttttg
    13801 atatattgtc tacttttttt acctcaccat ttattactat tgtcattatt gatatgactg
    13861 atataagact taatattaat agtcccaatc taatcttaat ttttctcatt gaaaattccc
    13921 cctcaagata aatttatatc atcaattatg tccaattaaa tttaatttat tcataatatt
    13981 ttttaatact aataaaaaaa gctatcttaa aatgattaca atcattttaa aatagctttt
    14041 taaaccttat attttataag tctttactaa tcatagcctt atctatcata aactgctctg
    14101 ctttttctat atgctctgta gcatacttct tggatagctc actatcacct ttaattatag
    14161 catcaagtaa atatttatgc tcttcaacaa tattatgtga tgtatcataa gcagacatat
    14221 aggtaactct aaatctatat acttgttccc acaatgagtt tattaattgg attaattttt
    14281 tattattagt tgctttaaat atacattcat gaaattctac atcaagtttt aataacgcat
    14341 ctacatcgga ttccaaagtg ctttttttaa attcttctga tatatcttta agctttttaa
    14401 tatcttcatc actaattctt tcagcggcaa gatatgctgc taagccttct aggcttgctc
    14461 ttacttctaa tacatccata atatccttta atgacatatt tgccacatac gcaccttttc
    14521 taggcaacat aacgactaaa ccttctaatt caagctttct tattgcctct ctcactggag
    14581 tcctacttac tcctaattgt tctgctagtt gcacttccat tagcctttga ccaggcttta
    14641 attttccttc taagattgct tctcttaaat tctcaaagac tacatctctt aatggcttgt
    14701 aattatccaa gttcagttta gttaaattct ccacaaattt caaccccttt ttgactgcta
    14761 ttaacaacat acacctgttt atattttttt aataattctt tttttccaat tagagcatct
    14821 tctttatttt taaatagtcc aaaaactgta ggaccacttc cactcatcat agaacctaaa
    14881 gcattatttt tcatcattac ttgttttata tcacttattt ctttgtgttt acctatagta
    14941 acattttcaa gaatatttac catgctttct gatacagctt ttatatcttc agattttaaa
    15001 cactctatca aatatttatt atttggtctt tttttaatat tattcaaatc taatccttga
    15061 taaacttctt tagtagaaac gaataagtct ggtttgcata tcaatatatt tgtatcacat
    15121 ggtaaacctt tgatgtttgt tagcttttct ccaatacctt gagctaaggc aggtcttcct
    15181 gatatacaaa aaggaacatc tgcgcccaaa ttaagtccta tttcttttaa ttcatcttca
    15241 gataatctaa gttcccatag gtgatttaaa cctactaata ctgcggctgc attagaactt
    15301 cctccagcca tccctgctgc tacaggaata tttttttcta tgaatatttc tacacctttt
    15361 tttatataaa atttattttt aagtattttg gcagctttat aaacaatatt atcttcatct
    15421 aaaggaatat ctaaactagt gctttttacc tttatttcat cttcgtctaa ttcttttatc
    15481 tttactatat cgtataaatc tatcgtttgc attatcattt caacaaaatg ataaccgtct
    15541 tgcctttttc ccaagacatc aatggataaa tttatttttg ctctactctt tagtcttata
    15601 aaatccattc tatcctcctt gtttaaagaa taatgcatca ctaacaataa tattatacta
    15661 caaaaaataa aataataggg tattataata ccctattata cttaattact ttaaattttt
    15721 taatattaat ctactaatac gacttttttt tctaaaataa gacacttacc aggcttaatt
    15781 ggttcatcaa gttttatatc attaagttct gcaatttcat tttctgttgt attgtatttt
    15841 tttgctatat tccagaaagt atctccttct ttgcatatat atacgattat gctaggagct
    15901 ttagataaat catatacacc ttggtcttct cccttgataa taaaattttc tgctttctta
    15961 tctaatgctt cagtaaatct cttaattttg ataatcaagt ctatctggtc cctattcaaa
    16021 tcaacttcga ctttatcaat acatgctgta ttaaacacag atgctgtatc agttagatta
    16081 tccatttcta tatcatgttc aaatggaatt tcctcactaa ttttataaac caattttaaa
    16141 ccttcaactg ggacaaataa aatttcaact tttatgattc cttgtataat gcttttattt
    16201 ccttcaatat aactattttc tatagacatg gttggacaaa cgcttacaat atcttttatt
    16261 tgtatatcat cattatcatt tcttatgcct tctctaacca taaatgtttc ttcactacgt
    16321 cttaaagtct tatttaattg aataggtttg tggtcaaatt ttattatctt ttgaggcgaa
    16381 tatgcatctt gtaatacttc tcttgtaact tcatctgtaa cttttacctt acaacaagcc
    16441 atacaatcta tttctaaaag acctgtgttg ctttcactat tttgcttgaa aatatgatta
    16501 aaatctgaca tagacaacaa tacttcttca gtcataccat cactaactcc agggacttct
    16561 acaaattgag taaactcaat acctactcta tccaactcta caagttcacc ctcatatgta
    16621 caagctaacg gatttatttc taatactcca ccaataataa ctttattgtc agtcactcta
    16681 ctttctttta ctttaacaca tggatttaaa cttattatag attgaatttc ctctgtgttt
    16741 attgttatag tatctcttat agaactttct gctttttcta ttccaactat atcttgaaaa
    16801 caaatttcct ttctgtgctt ttgtatgcct tccacttgcg ccacatcttt tacaatgtct
    16861 aacctttgct tttcaaacaa acttcccctt atattcataa gagctcctac tttaatcttt
    16921 ctctcattca ttatagtaca atccatatgc tctacttctg aaaataacat gtattccata
    16981 tcttgaacaa cattgtcttt ttctattact tcgtttatat ctacttttcc gtctacatta
    17041 gaaatagtat ttttatcatc tgcaatatag attacattat aattaaaact acctctgcaa
    17101 agtatcttcc catctgccat ttcaattttt ttaagtgata tgtatccttc tgtttttaca
    17161 atttcatata catctaattt tttgtctggt acaactgcct ccgcttcaat gaatgtttga
    17221 aattttccaa agtctattct attgtcaact ttaattacat ctttaattaa ttccatagtc
    17281 tacctcctta agtattataa aaatatcaca ctatatttat atttaaggag caaaaaaaaa
    17341 attacaccta tttttaaata ggtataattt taacttattt gtaatttatc ttgatctcta
    17401 aaaacttgca actttacatt agatgttaat aaatctgagt aactataaga tactctagga
    17461 tatccattac tttcatcatc taactttata acaaacacgc ttggataaac tttttctagt
    17521 ataccctctt ttgtaataat ctgctttctt cctttattag ctttcaacaa tattttcttt
    17581 cctatatgtc tctctaagct tactcttatc ttatctagag tttgaacagt agccacaata
    17641 tcaccttctt taacgtttat atgtttacat aatatcacat ataaacattt ttgtcaaatg
    17701 tttaaactaa ttattttata tattttttgc aaaaatgtca atactatttt tgaagatata
    17761 cagtataatt atgttaattt ttgctaatgt aatattgttt ttggcattgc ccatctaaat
    17821 tttcctctgg tttcagaacc accaattccc ttaacacctg taatagtctg ctctataacc
    17881 tcatcaatag gaactactac atcaattctt gaacaagcaa ttttctccac tataaaaaca
    17941 gggtctaatg catgtatcat tatccttcca ggcgaacttg catagtttgc tcctgcactt
    18001 attatttttt cataatttga ttggcatgct cctgcgaata tgacaagatt atccaaattt
    18061 ggttgccatt tacgtgcttc ttttactgtt ttaacaaaat ttgaagagtt tctatagtta
    18121 ctcatatctt ctatatctcc tctttttatt gtcattgcat catgacctgt tattactagt
    18181 atatccggat tatatttttc tagtagtgct cttacttctt tatactgatt ttgctctgaa
    18241 acagctacac ctactgcagg tattccaagt tgagcatata catccaagca aatttttaaa
    18301 tattctttat ctccatctat ctgtagtact tttccaggca ttccatatgt gttaggattt
    18361 aattgaagtt ttggaactgc tctagtcatt cttttttgtc tttctttagc ttttcttact
    18421 gacttatata ataaattttc tacattttta tctattaata tatcttttat atcaggtgct
    18481 tttactaatt ctaggtcatc aatataagca tctgctatta ctctaaatgc aattcccttt
    18541 agtatagcta ttttctcatt attttcatct acaccaaaag agactatttt aaatactata
    18601 tctttgttat gtgactttct agctacaatg tctcctacct tcatagttaa acctccaata
    18661 aatttttatc ttagtttata ctatgttcga tataaaactt tgtttcctaa tgtcaaaaaa
    18721 acatttctta taatacttct tttatcactc ttaaagcatt tttatagtat atcttttcta
    18781 tatcatcttc actaaaccct tcttttttta gaggttcata cagtttaccc atctgagata
    18841 tatcttcaat ctcaactttt gaatctattc catcaaaatc tgaccctaga gatacaacat
    18901 ctattcctcc aacatttaca atatgtttta tgtgtctaac catatcttca agtttacttt
    18961 cacttttatc acttaaaaat aagtggaaga agtttatacc tgtaactcca cctttatttg
    19021 ctaatacttt aatcatatca tcagttaaat ttctagaatg attcatcatt gctcttgaat
    19081 tagaatgtgt agcaattatt ggtttagaag aaatctttgc aatttcataa aatccaccat
    19141 ctgaaatatg agaaacatca accaacattc ccaattcatt catcttatgt accacttctc
    19201 ttccaaaatc agtaagccct ttttctctat attctacttt attatgagga aagctcaatt
    19261 cattcacatg attccaactt attgtcatca ttctaacccc taagtcgtaa aatttcttta
    19321 aattttctaa tttaccttca agtactgcac cttcctcaat agataaaaga gctgtaagtt
    19381 taccttctga ttggtttttc ataatttctt catagttagt agctaaagct atttcatcac
    19441 tatttttttt catttcctca tgaaatttat ttgccatact catgcaataa tcaaaaggat
    19501 gttttacttc ttcagtatca acaaaaagag caaatgtttg agctaatgaa tccccctttt
    19561 tcaatctgtc tatatcaaca gaatatttat tagacttaag ttcacttgtt tctacatttt
    19621 ccattaattt tgctatagta tcacaatgta aatcaataaa tttcatattt ataccccctg
    19681 tatattttat atttatatct aaaaatagaa atagttgtcc ttatagacaa ctattgacaa
    19741 ctatttttta atattctttt taatattact taatcttatt cttcttttaa gttaggtatt
    19801 acttttttaa cttcttcaat ttgatgccat gttttatcta tattattttc actaatatat
    19861 ttttttaaat cagttaaaga tgtaaaatct ttaactgata aattgtcatc atcatctgca
    19921 aatcttaaaa taaacatgag actcccttac tttccacaac atttcttata tttttttccg
    19981 cttccacatg ggcatgaatc atttcttcct atttttgctt ctttaactat tgttttagac
    20041 ttcttataag cctttattat ttcttttctt tggtctgctg gtagtatatc ttcccaacct
    20101 tgtaaattat atagccaatg agcctcaaca gttaccatat tgttatataa tttttcccaa
    20161 tctattttta tagaaacttc tttatcagct tctacttctt ctaaaactat atcttctttt
    20221 aagctttcac ttattccatc tatgaatccc atgaaaaatt cattagtagt tccaaatttt
    20281 tcagccaatt cacttacttt accttcaaca acatccattt tattatttaa tatttcattg
    20341 tatattccag cttcaacttt caaatattct tcccagaatt ttacttctgc ttcttgactt
    20401 tcatgattat cacttaaatt tctccattca gtatataaac tcatactttt tcctcctaag
    20461 tatacctata tttttatagt ttttatagta taaaatcttt tagttagatt ggaagtttct
    20521 tctttttgat tataccatat tctatttaaa gttaactctt atattatatc ataattaaac
    20581 aattctttta atacttttat agcatactct tggtttataa aataagggct tgtcgcaatt
    20641 gttattactt tggctttttt aattaagttt tttatcttgt aaaatttgag ttcaaatggt
    20701 atatagtcca tatcacttga aaatatatcc aaatctatgt caagaacaaa ctctgattct
    20761 acttctaaat caaatccata tagactatct attattatta attctgaaaa tatgttatat
    20821 tttaaagcag gttttataaa acttcccaca tttaaaactt catttgtata tctaaataca
    20881 tcttttagat tatttacatc tacatcatag ttctcaggtt ctctagtatc cttatgttgg
    20941 tctacatgca ctaacttgca tccttttgta aactcaccct ttaacaaaga ctttgcccaa
    21001 aaataaaatg catggttatg attgtcaaac acatatatct ttttatttcc caggacatat
    21061 tcgaccatat tctccaatcc ttttgctttt atttccttat tttcatctac ttcattaaat
    21121 actacctcat tgcctaacct tacatcattt aatgtaccat ttataagttt tggaacgtat
    21181 atgctcttat tttctctttt atcatatgaa aatacattat ttcctactgg tttttctata
    21241 taaaatccac tatattcata attttccata aaactttatt tcctccattg ttatataaat
    21301 atataagtta taattttaaa aattaaaatg cacttattgt aaatcatatc atctattata
    21361 gaaaacatct tatataacta aatataatat cttctctcaa tatgtattat acaataagtg
    21421 taaaatatat ttatataaaa tatactacat tcctataaca aaaccatcag cttcaattgg
    21481 aagataacta actcttctat tcaactccac ctttccaaaa aatactaccc aatatggtct
    21541 aaaaatactt tcaattttaa ctatttcaat ctctgggatt cctcccataa acctatgagt
    21601 taatctcata gccattttat ttgcctttgc ttttactctt cgttcgtcct tatcactata
    21661 ctgcacaata ttacaatcca cattttttac aacttcatat gtaggaaaat cttgtactat
    21721 actaccagaa cctgtagaac cattcactaa aatttttaat tcatttatat cattagaata
    21781 tctcctattc ttgaaaatgg atttcgtttt gtgaatttta agtgtgataa ttttaaactc
    21841 tatataatga agtctaactt catgagcata tttgttcttt aatattagct tactaaataa
    21901 agaatttttt ccacttttag ctatctctat agcttcttct tttgtaattc ttataggtaa
    21961 cgctcttata tccataaaat cattctcttt cttatttttt tcttgtcatt tttaaaactc
    22021 tatatatata atcaaaaaat attacaatta caactgacat aactccatat ggaattattt
    22081 ttccaactat aatatcattt ctagtcaata taccataaaa tataaatcct gttattaaaa
    22141 agtacaataa tactagaaaa ccgtctattc caagccattt tatttctaaa tcttttttta
    22201 ccttagacac taatttagac atagcagttt ctccttttat agattatttt gtgtatttaa
    22261 attttcacct attctattac caatcatcca tgcaataaat gaaaaaggta tagctatcac
    22321 aagaggctct aactcaaagc tctgaactgg aaatatttta aaataaccaa aagtcactcc
    22381 accaacaatc attcctaaaa taccacccat agatgttctt cttccatttc caagaagccc
    22441 acctactata ggtacaaatg ccgaacccat ccaaatactt cctacaaata tacatgcatc
    22501 atatacattt tcaaatctaa atgctacgct aagccccata actgctgcta aacatacacc
    22561 tattctagta aactttacta attgtttctg agataactct ttttttgtaa ttcttccata
    22621 tatatcattt gctaatgtag ccccagctgc tagataatag ctatccaatg ttgatattat
    22681 agaacctaaa attcctatta taaatattac tcttattcct tgtggtaatg tatctaaaac
    22741 taaagttata tatccagcag ctggagccat ttctggataa gtagctctta ctataagtcc
    22801 tgtcatagtt aacaccatgt caaaacatat ccatagtgaa aaacaggtta ataaggctct
    22861 tcttccaacc ttaggagaat ctgctgctga aaatctttga taaaaagttg ggtctgaata
    22921 agctccaaaa gaatatacaa ttaacatcaa tgctttcata gctgacatac cctttgtagg
    22981 gtgcataagc tgtggtgcat ctttgagtgc ttctgtaaga ccttgaaacc ctcctatttg
    23041 ttcaaattga cctggcataa ccatagttaa accaaatacc atacaaaaaa acaataccat
    23101 atctgtaatg gcaactccca taagcccacc aagtactgta agtacaacga ctactcctac
    23161 cattattaat cctattaatt ctttatctat tttccatgct acgttaccta tagttcccat
    23221 agcagttatt tctccaagtt gtgcacaata tataaacatc aaaattgccc caattatagc
    23281 aacatgcttg ccataaaaac tttccagtaa atcaggaact gttatattgg ttcttatatg
    23341 aatccttgga ccaacccaca atgctaaagg cattctccca atatgtgcag ctattgacca
    23401 aactgcccat gcagatattc catatatagc cgcatactct acactaccta ctgtaccaac
    23461 tcctccatac catgatgcaa ccaaagttcc tactattaag gaccatggta aacttcgatt
    23521 tcctaggtaa taactttcca tatcttttac atttctagaa aaataataac ctattcctaa
    23581 taatataaat gcaaatataa ctataatccc attttcccaa aatgttaaca tatctaatct
    23641 ccacattctt atattttaat ttttaacttt aatatctaaa tatgaacaac tctacccatt
    23701 tccataaaaa taggtagagt tatataataa ttataagtat ttaagtaatc ttgttattaa
    23761 gcaattttat tgtttaaacc tttattttct cttttattta ttttatttaa agcataagta
    23821 gcaaatatcc ctataactgc acatactaca gataaaataa atatatatgt atatcctgtt
    23881 tgtccatatt tatctaacca attacctgca atagtgtaat aaaacgcatc tggagaaaat
    23941 cctataaatg aagcaaatcc aactactgct cctgtagttt ctaaagatat attagactct
    24001 gaaacagatg caaagtatat acctctaaat ccaaataaaa ttacacttag tattatcatg
    24061 tttattacag ctatccatat aaatcctgta ctcttaggaa taaccaagta agcagctgta
    24121 cttactgtca ttaatataaa tcctacaaac attactctta tagatgaacc aactttatct
    24181 gttataacac cagcaactgg agatgctccc atttttataa cataagttct tattattgat
    24241 aaagcagaaa ctagagtcat tggcattaca taaacttctg ataagtatgg acttaaataa
    24301 gttaaacttg agaatactaa ataagttgaa aatattataa gaccaattag ccaagccttt
    24361 ggcattgtaa ctacttttaa caatgactta atacttaatc cttcatctga tgctccacta
    24421 tcttttttct cttccactaa gaaaaataga agtataccac atattataga tgctgcagaa
    24481 tataaccata ctacatattt gaatcctact gttatttcag caaatttacc aaagaagtaa
    24541 agacctataa aagaaattaa tgtacctgat acacctgata acccttcata aaaaccaaat
    24601 attcttcctt gctctgactc acttccttgc attctaacaa ccttaacaga ggctgagaag
    24661 aaagttaata ttgttgtaaa tccccataat acaaatatta aaagtatcat actatatgat
    24721 gggaatgtag agaaataaaa tccaagtatt cctgaagcaa ttaaagaaaa agttattaat
    24781 tttctaactg agaatctatc tgcaaaccat cctcctataa aataagatac cattcctact
    24841 aatccatata aactcaataa gttccctatt tgtgtatgac ttaatgctaa agcttgttgc
    24901 attgggtcat aaaatgatga tttcatatat ggtaaactat acatagctgt tgttccaaat
    24961 gccaatatga atattatcaa aaatctttta acattatttt tgctttccat tgattctctc
    25021 tccttaaaat tttattgttt ataatctcat tcatataatt ttattttaat ttttctattg
    25081 cttgtttaaa atcatttatt atatcatttg aagattctaa tcctacagct attcttatta
    25141 gattttcccc attcatttga actaatgtat gctcttcccc taagcttgtt gctattgttg
    25201 caagctctaa agaattcatt aatcttctac tagcttggtc tcttgtcaat ccattgatac
    25261 catccttaag tttaaatgaa actacacctc cacctaaacc attcatttga gttttacaaa
    25321 gctcatattg aggatgagat tttaatgctg gatactttac caattccaca ttctcttctt
    25381 cctctaaaaa ttctgctact gctaatccat tttcacaatg ctttttcatc ctcatatcta
    25441 acgttttcat accccttaaa atcatccatg cactcattgg tggtaaagat gctcctgtaa
    25501 aacaacataa acttggccat ctaactaaat ctattaattc ttttgaacct gctactgctc
    25561 cccctaaagt atccccatgt ccatttataa acttagtaag actatgtatt actaaatctg
    25621 cacctaaatc tattggtctt tgtacaattg gtgtcccaaa agtactatca actatagcta
    25681 agcttttatt ttcatgagca atttctgcta ttgtttttat atctactaag tctaatgatg
    25741 gatttaaagg actttctata taaactattt tagtatttgg tctcattgct tcttttacat
    25801 tttgtggatt tactgcatct acaaatgtaa cttcaactcc aaatttagat aacaactcag
    25861 acatgaataa tttagtgtgt gaaaatattc catctgaact aactatatgg tcaccacttt
    25921 ttaccaatgc taatatagta gatgttattg cccccatccc tgatgtacta gatatggcac
    25981 tttctgcatt ttcaagcata gccaacttag cttctaactc tgtaagagta ggattacact
    26041 ctcttgtata cacatatccc catgtcatac acaactcttc aaaatgctct actgtctttg
    26101 cagcaaatgt tgctgtttga catattggtg tagccaatgc acctgttgtt gggtctgcac
    26161 tatgtcccca atgtattatt tttgtctcta aatcttgatt aatattgtta ctcattttga
    26221 tttcctccca ttttgactta tttatacaac gttttcccta ccaaatttca tcaatttagc
    26281 ttagtcttaa tacatttttt caagtatact tttttatttg ttataatttc taaaaatatg
    26341 acacttattt tgttttttta cagttctaat tataaactct atagtaacta tagagtcaat
    26401 atgtttttga tatttttttt acaatgttta aaatagtcag tacaaaaata ggtatatctt
    26461 agaataacta aagtcatcct aagatatacc tatttttata tcacaatata aataattaca
    26521 tataagcatt agttttttta tcataaatag atttttcaag atttatcatc ttttcataaa
    26581 tatattatag ttaaatattt taatattacc taacatctac aataaaatat actcttaaaa
    26641 taaaattata taattaaaaa tactctataa aagctaaaaa gtttttctat tcgctacatc
    26701 taaaaataat tagatagagg tataaatgta tttagtttat ttatacctct agtcttttat
    26761 aaattatatc tctactaaat tgatttttta gcacgaataa gtgtgctctt ggagatacca
    26821 gtcatcttca caacctctct atatggctta gaatttaata accccaatgc atattgtatc
    26881 tgttgattgc tgtatttctt tggcctacca tctttaaagt cctttttctt tttagcaaat
    26941 tcttttccta attgagtacg ttctattatc atatttctct caagttctgc aactgcaagt
    27001 aatgtagtca aaaagaattt acccattgaa gtattttcta aaaggcctat atttaaaata
    27061 tgaacactta cttctttagc aaataaacct tctattagtt gtattccttc tgatgtactt
    27121 ctagctagtc tatccagctt agttgcaaca agtaagtctc ccttttttag agatgatata
    27181 attttttcaa atttaggcct gtttgtagta gtgcctgtat aagtttcctt tattatttta
    27241 gcatcattat atttattcaa aatttcttgc tgttgctgat acaaactgtt gttgtctgtt
    27301 tgattttttg tacttacacg gcaatatcca tatataataa tcatcacttc ctaacataaa
    27361 gattttgtac agaagttacc cagaagcttg tttttactta tctttatcca ttttactaaa
    27421 acgatttttg ggtgtcaata ctgttaactt ttgacaccat aataattcct ttataatttg
    27481 tttgactatg tcaaaaatta ccaatataat gaaattaatg agtttataca aattggagga
    27541 aataatatga atagtctgtt aaaagaagca atagatgaat taaaaaacat ccattgcggg
    27601 gaagtttttt tattaaaaga attgttcaaa ggctatgaat ggaacaggtt ggagatgaat
    27661 atcagattga aattaggaaa actttttttg tatgaagtgg taactaatcc caatttaaat
    27721 gtagaggtca tcaataaaaa ttctgcttat cagcaaatgt ataaaaaaaa atagtgactt
    27781 aaatgtatat aatagtataa tcttcttatt gattcaaaag ttaaaacttt taatacaaaa
    27841 caatcattct attttaacat ttatcaatat gtctaaattt atataattaa cacatacgaa
    27901 tagtattgtt taaatcacat tatttaagat agataaatct taaccctact ccagcataaa
    27961 tactaaaata gggcttagaa ttatctatat ttttatccca taaaatgctt agctcttcct
    28021 cttgattgaa catatatatc tggggaaata caatagaagc gtaaatttat aaaatttcca
    28081 aagtcagaaa acttcagttc aaacttaggt ataccaaaac tttcaggtgc tatttcgtca
    28141 acgccaaaat agatgataat tccattatct gtaagataaa atgcttggtc ttcaggtata
    28201 actaactcaa cattagaata atacatttct ctattttcat ttatttgaga tttaatgtaa
    28261 tcagtaatta tattaatata atcaactcca tctctaaaaa tgtcattaag taaaagttct
    28321 tttccagttg tcaaatctat attgtaatta ttcaatgtat tgtatttagg cccatagtta
    28381 ttagcaaacc ccataaggtt taatatcata ctcagtacat aatttttatt aaaagtaact
    28441 gcgtaactgg tagataccca atagctttgt tttggaagtg aattttgaga agttgcgtta
    28501 ttatattctc gttcttcttc ctgtaagcca tttttaaaat tatttacagt cattcttaca
    28561 gcattgttta tgttatttaa aataactggg tttacatacg tggattgatt tctaatattg
    28621 tatataacag gatactttac atcatatgaa aaatacttac ttctttcaaa atcttcatta
    28681 aattgtacat ataatatatt atatgtacag gtatttgtag attgataatt ttccattaaa
    28741 atcctccccc ttttataaaa aaatcagata caatataata tgcttaaaac agtaataatg
    28801 ttaatggtaa atgtaaagaa tagaaagtat ctaaaacagc aaaaaatgac taattttgtg
    28861 gtataataca taggattgag agttatttta taggaggaaa aatatgttat tattagcaga
    28921 taaatggaaa gattatgaac ttatagatat gggaaatgga gaaaaattag aacgttgggg
    28981 aagttatgta cttagaagac cagacccaca ggttgtatgg ccaatggaat ctgaatgggc
    29041 tttatggaaa aaccctcatg gacattatca cagaagcaat aaaggtggag gtcaatggga
    29101 acacaaaaaa agatatccag aacaatggac tataaattat aaaaacttaa agtttcacat
    29161 taaaccaact ggctttaaac atactggatt attcccagag caagcagcta actgggattg
    29221 gatgatagag aaaataaaga aagctaacag acctataaaa gtgttgaatc tttttgcata
    29281 tacaggtgga gctactgtag catgtgcatc tgctggtgca gaagtatgtc atgttgatgc
    29341 tgcaaaaggt atggttaatt gggcaaaaga aaatatagaa ttatcaggtc ttaaaaatca
    29401 aacagtaaga tttatagtgg atgatgttgt aaagtttgtt gaaagagaaa taagaagagg
    29461 taaaaaatat gatgcaataa taatggaccc tccttcttat ggaagaggtc ctaaaggaga
    29521 agtatggcaa atagaagaaa agttatacag ttttgtagat ttatgtatgg gagttttatc
    29581 agataaccct ttatttttct taattaattc ttatacaaca ggattttctc caatagttct
    29641 tgaaaatgta ttgtctacaa caataggtaa aaagacaaat acaggagata tatacggtgg
    29701 ggaattagga ataccagctt ccagagatgg taagatactt ccttgtggta tatttggaag
    29761 atgggagaaa aaataatgat aaaagtcata tatgaagata accatttatt agttgtagaa
    29821 aaaccagtta acatattgtc tcaaggagat aatactaatg ataaagatat ggtaaatctt
    29881 ttaaaagact atattaaaaa aaagtataac aaacctggaa atgtatatat tggtttaata
    29941 catagattgg atagaccagt aggtggagtt atggtgtttg caaaaacatc aaaagcagca
    30001 tctagattgt ctgagcaggt aagaagtaag actttcaaaa aaacttatct agctgttgtt
    30061 catggcaaga taaaatctaa ctcagatata ctaaaagatt ttttatataa aaataaaaaa
    30121 actaatatgg taactgtagt gaaaaaaagc cataaagatg ccaaagaagc tgaacttaac
    30181 tataatgtat tagattttaa agaaaatcta agtttagttc aagttaattt aaagactggt
    30241 cgttctcatc aaataagagt tcaatttgca agcagaaaac atccattata cggagaccaa
    30301 agatatggca aagaaataaa taagattgga caacaaatat ctctttggtc caataagata
    30361 gaaatttgcc atcctactac aaaagaaaaa atggaattta catgtcatcc acctgacaaa
    30421 tatccatgga atatatttta aatctcaaat gaggtgatta tatgagtaaa aaattttcaa
    30481 taggagaaat gtctaaactt cataaaataa gcatacagac actaaggtac tatgaccaaa
    30541 tagatttatt taaacctgtt catgttgacc aagaaagcaa ttacagatat tactcaattg
    30601 accaattttc acaattagat attataaaat atttaaaata tttaggtatg cctttaaaag
    30661 aaattaagtc aaagcttgat gaaaataatg aagaaattct aaaattacta acaaataaaa
    30721 ttaatatagt ggatgaaaaa attaaagaat tagagcttat aaagaaggta ctaagcacaa
    30781 agaaagaaac tatggaaaaa ggtatgaaag tagatgaagt tggtaaaata gtgagaaaac
    30841 atattccacg aagaagtatc ttatctatag agtatgaaga aggattaagt agtgaagaaa
    30901 ctatggaact ttccagaaga caaatagcaa atatattaga agataatata tctgtgttct
    30961 atggtggagt aagtggactt gtatctatag ataaattttt aaaagaagat aaagttattt
    31021 atgacaatag ttttgttact gttgaaagag acttatttga tataaaatca caaaaacata
    31081 taacagaaat ttcatctggt gattttattt gtatgacata taaagggcct tacaaagaaa
    31141 attataccgc ttttaagaaa ctggttgatt atgttgaata taaaagaata cctattgaaa
    31201 attatatata tgaaatacct ataatagacc ctctgtctac aaacaacgaa gaggaacttc
    31261 taacagaaat tcaaatacaa gttaaaaaga gagaaattga cttaagatgt ctagtgtagt
    31321 aatctagttt acttacacat cttaatatag ttgtctatta tcaacatatg atttttctag
    31381 atagtgattc ctaacagtga gattttcaaa aaataggagg aaatatggct aatgctaatt
    31441 ccctcctatt tttaattaag ccatatcatt ttaattaagc catgtcatca cgaagtgcat
    31501 caataatatt ttcttttttt atcttattaa tagtatacaa cattgtaata aatactataa
    31561 ataaaacact aaatatacta attccaatac taaaccatgg aatcacaaaa tcaatatcat
    31621 ctgtaaccat tactttatga attagccatg aagaaattac tgctatagga agtccaaaga
    31681 acaatgctct catgccataa aaggcacact caaaattcat catcttttgg aaatcatgct
    31741 cagacattcc tacagagcga agcatggcaa gttctctacg acgtaactta atattagttg
    31801 aaattgtatt aaacacattg gcaatcgaaa tcagagaaat cataattatg aaagtataag
    31861 caaaaacatt ggcaataaaa atgtaattac ggttctcatc catcattcca tacatattgt
    31921 acagattgta ctcattcgta actccctcac tctcaataat ttgctccatt tctgcaactg
    31981 actgagtagg atttttcgat tgaaagctta aactttcaat tcctgtagaa acaccaggaa
    32041 tctcaaactt atctttggct gaataaggag ccaatactct gaagcttgct ttcttcacat
    32101 ctgaacttct gattcctgga ggagaatcag gtggcactac tttgacaaat gaaatattca
    32161 cattttgtct ctcaggtatc tccaacttgc cattagtttc aggagtgagg ctaacattca
    32221 tagaagaact gttgaaaata tcaataatat tattatcctt tgttaccata gaaacagcaa
    32281 tcagttttgc attttgtcca gtatattctt ctgctggtaa tccaagactt ttaataatat
    32341 tcagataagt tctatcatca agaaattgca tatctactgg taattcaagc atttcactca
    32401 tagaatgtga accctcatag ttcaaataat cgtctgtaag ctcacttact ttggcaacac
    32461 atgaataggt cataagtttc tgatacaaac cttcgtaaac accatttgca gtttttagct
    32521 tatcgtaaag cttcaacatt tcgttgtctt tcataccttg tgtagaaaaa ataatatcct
    32581 ggtcagtaac ctctgacctc tgatttgaca tctgttttag gtctgctact aaagcacttg
    32641 tagatataaa tagaactatg cttaaaacaa gcgacaatac aatacttcga taacgtttct
    32701 tgtttctttt aaagtttttc atagcaagaa ctccctcaag accataaact cgctgtgtta
    32761 gttttgatgt cttcacagat ttagattcaa tttttacctc atttgtctga cgaatacact
    32821 ccatcactgg tgtgttggca gcctttcttg ctggtatgta agcagaaatt aagattgtaa
    32881 ccatacttac cactgctgca acgatgattg cagggataga caatgttaat gtcaacggaa
    32941 cgtttacata gagaatattt gcaaagtttt tagctatgac agaaatcaca agtccaatac
    33001 tgactatacc gattgcaaca ccaattggta taccaatcgc accaatgcaa attccttcaa
    33061 atagcaccga atttcgtaac tgttttgctg tagctccaac agatgaaagt attccaaatt
    33121 gatgtgttcg ctcgttcaat gacatgttaa atgaattata tattagaaaa attgagccta
    33181 gcatgattat ggcaagcaca atgccaccaa ccgagtacaa aaacatattg aacaaattgt
    33241 tatcagaaac acctatgaag cgcaacacat tgtcattgaa aatggaagca tggtctttag
    33301 tgctacttat gtaagagcga atctgatgtg gatttttcaa tgtgacaaaa aggctaagac
    33361 tgtcagtctt gacttgttta tcttctttgg ttatcaaggt atatcctggt gcagaagatt
    33421 cttcaaatac gggctttcta cagataccca caactgtata agttttctta tcctgtggca
    33481 caaaaacttc actcccagca gtgtatgggt cactctgacc aagcttttca ttttcactca
    33541 ttcgtttccc tacatccaaa gaaatagtat cacctactgc aaatttgaca ccaccatctg
    33601 tcgcaacttt acttgaaaca agaatctctc tactattttc aggtaatctt ccagaaatta
    33661 aagttatagg caaagtatca aaagcttctt tgctaaatcc agcaataaaa agatatggtt
    33721 tatctggatt tctgttacct tcaagtgttg catagccaat attttcaaat gttgcagtat
    33781 ttgcaacttc atcattgact ctttgttcct gtacaaaaga agaatccaca tccaaaaact
    33841 ggacatgcca atcaccatat tttataatag ctccatttgt catatagttc atcaaagaaa
    33901 ctccaaaggt agtaactgct gtaatcatag cagctgacag aacaactcct ataattgtta
    33961 caagagttcg agtgcgattc tttttcaatc cttgcagagc aactttgtta aaaatgttca
    34021 ttaacgattc accttcctct catcacgcac tacttttcca tctgaaatac agataattct
    34081 atctgcctgt aaagcaatgt tttcatcatg tgtgacaata atgagagttt ggtgatattt
    34141 actatggctc tctttcagta actggataat ctcttgtcca tttttactgt ctaagctacc
    34201 tgtaggctca tcagcaagca taactgctgg tgcattcatc aatgcacgac caatggcaac
    34261 acgttgctgt tggccaccag aaagttgatt tggtaaatgt gtttttcgtt cttttaaacc
    34321 aagcaattct aacaagtcat ttaaccgttc ttgattgacc ttacgcttat ccataagtat
    34381 aggcaatgtg atattttcta ctacatttaa tgtgggtatc agattgtgaa actgataaat
    34441 caatccaact tgtcgcctac gaaaaatagc aagtttttca ttgctttggc catatacatc
    34501 ttgcccctcc agatatatct ttccacttgt tggtacgtct actcctgcaa tggaatgtag
    34561 tagtgtagat ttaccagaac cagaagaacc aatgattgca gtaaactctc ctttttcaat
    34621 agtaagtgag acatggtcaa gtgcagtaac cttattttca ttcttaccat aaaccttaca
    34681 taaatcttca atctttaaaa acgccattat ataagtctcc tttcatattt tataaatata
    34741 tcataggact ttcaacttac atttaagtga catttaaatg agagattcgt cactttggaa
    34801 aacgaataac aaatattgca ccaccttgtg ggtgattttg tgcagtaatt gttcctcctt
    34861 gtctcataat aatcgtctta caaagtgcca atcctattcc ataaccggct gtgcttgaat
    34921 ttttcccacg ataaaatctg ttaaatagac atggtaaatc ttctttttta aagcctgctc
    34981 cgttgtcatg aatagtaagt tgagtaaaca aaaagttgtc ctcacaaatg atatcaattt
    35041 ttccattatc acccacactc tccatgcagt ttttaaatat attttgaatt gcttctgaaa
    35101 gccaatttaa atcacccaaa atccttactc cttttggtat atcaatatgt aaagtgatat
    35161 tgtgcagttc catgggaatc aaaagttgat gaagtgcaga actaatcaaa ttattcacat
    35221 caatttgttc tttttgaaag actacaatac ctgcatctaa acgtgataat tttaaaaggg
    35281 aagtaatcag ccagtccatt cgtataagta attcctctgt ttctcgtatc aactcttttc
    35341 tctcatcttc atcagaagta ttctctaaca gtgacaaaat aagagttact gatgtaagag
    35401 gagtgcgaag ctggtgggct atgtcagcta gtgagtcagc aaggtgttct ttttcttttt
    35461 tcaatgcgta attttgctct ctaatacgta acgtcatttt agttatttca ctttgcagaa
    35521 tagaaagttc accctcatct gattcagcaa tatacagatg gttagcatta tgaagcacaa
    35581 gattaatttg ttctgaaatc tgtgcaatac ttttgtatcg atttttagta aacacaaaaa
    35641 atgctattcc aaaggtagtg gcagaaacaa tagaaagtat tccagtcata atactgattg
    35701 caaatcccag ggctacagtg agaatagtaa ttaataagaa taaaatagca aattttcgaa
    35761 actctctatt tcgaaacata acttcccccc aatctatacc ctgtcccacg aacagtcaaa
    35821 attatttgtg gacttgaagg gtcatcttct atcttctctc gcaagcgttt gatgtacaca
    35881 gtcaatgtgt tgtcattaac aaactcacct gccgcatccc acaattcatc aagtaatctt
    35941 accctcgtaa taatactttt aggattgcta ataaacacca ataacaaacg gtattctaaa
    36001 gctgaaagaa aaacctcatt gccattcttt ttcacaacac cggttgccgt atctacataa
    36061 agcccacaaa tttcaaaagt tgacccaaaa cgcccacttt ttcgtagtgc tgttctaatt
    36121 cgcgcaatca gttcacgtgg acgaaaaggt ttagtaatat aatcatctgc acccatattc
    36181 agtccagtaa caacacttga ttcatcaccg gaagctgtca aaaagataac aggaatgtct
    36241 tgcttttctt tgatttctgt gcaaatcgta aacccatttc catcaggtaa agaaatatca
    36301 ataagtgcca aatcaaattt attcttagca atctctacaa gtgcttcact tcttgtggat
    36361 acatgggtta ctataaatcc ttctgaatta agcaaaagaa taagattttt ggcaattgct
    36421 ttatcatcct caactaaaaa tatccgtttc atttactttc atcctccttt attttattag
    36481 ttttatatat tccagcagag caatagtaca ctactatttt acttatcatt acacaatttt
    36541 cagtagtttc ttttgttctt atataatcca tgcataatac ctccattgat ttattatatt
    36601 tcttataaca taaatacaca agataagaca aaaaacataa tgtaagtgca agttttttaa
    36661 ctcactaact tataaacata cactcctact aacactactt tcttgatgac acatcttaca
    36721 agcttatatt catacatcaa tgaaaaatta aatttatcca tttattaaaa taaaaggcaa
    36781 tagcttctat agctaatgcc ctttatctta aaatgattta aatactatac aatttttata
    36841 aatggctata aataaactcg tatagaatat aaaatttatc tacagtcttt tgttatacaa
    36901 aatttataac ctcttataga acaaatttac cactttgcaa acttatcact tttactcagc
    36961 tatattataa acttttaatt tatctttgtt taaatgtttc ttcttaactt ctactaaagc
    37021 ttttagcgta tccaaagatg tcattatttc agtagaaaat tcaatggctg ttcttctcat
    37081 cttaaatcca tctcttgtag agtcatttcc tttagttggt gtatttatta ctatatcaac
    37141 ttgtttattt cttatagcat ctaatatgtt tggtctaggc tcttcaattc tatttactat
    37201 atcagcctct attccatttt ctcttaaact cttcgctgtt ccttctgtag caacaaaagt
    37261 atatcctaat tctttcatat cttttgctat ctctataaac tcatctttat catgattatt
    37321 tacagtagca agtactactc ctctttcatc agacatatgt ctacctgctg ccaagaaccc
    37381 tttatataag gcttcttcta aattttctcc tacacctaga acttctcctg tagacttcat
    37441 ttcaggtcct agacttactt caactttaga taatttagac attgagaata ctggtacttt
    37501 tactgatact aattttggct ctttgtaaac tcctgtacta tatcctaaat cctttaattt
    37561 agctcctaac atacacttag tagccaagtc aactattggt actccactta ctttacttat
    37621 atatggaact gttctactag ctctagggtt aacctcaatt atatataatt cattttgaaa
    37681 ttctataaat tgtatattaa ccataccaag cacattaagg tctaaagcta tttttttagt
    37741 gtattctact attttttctt ttatttcatc agatatattt tgacttggat acattgttgt
    37801 actatctcca gagtgaactc cagctctttc taaatgctcc attataccag gtatcaatat
    37861 atcttcttta tcacatatag catctacttc tatttctcta ccagttagat atttatctat
    37921 aagtactgga tttttatggt ctctatcaaa tgcatcatcc aaatactgaa ttagtttttc
    37981 ttcgttataa gttatttcca taccttgacc accaagtaca taagatggtc taactaatac
    38041 tggataacct aactccttag caatttcaac accttcatta gttgtccata caccttttcc
    38101 ttttggtcta tttatatcta gtctctctag taaatcatca aatttttctc tatcttctgc
    38161 tgcatctata tctctaaagt ctgttcccag tataggtata tttttttcat gtaaaaactt
    38221 agccaatttt atagctgttt gtccaccaaa ctgtagtatt acaccttctg gtttttcttt
    38281 ttctattata tttaaaactt cttcttctgt taatggttca aaatacaact tatcagatgt
    38341 atcaaaatcc gtacttacag tttcagggtt attgtttaca attatagttt ctattcccat
    38401 ttttcttaaa gattttacac agtgaactga acaatagtca aattctattc cttgacctat
    38461 tcttattgga ccagaaccca atactacaac ttttctctta tcactaacta ctacctcatc
    38521 atattgttca taagttgaat agtaataagg tgatagagct tcaaattctc ctccacatgt
    38581 atcaaccatt ttataaactg gttgtatatt atataaactt ctcaattcat ataatttttc
    38641 tgggcttatc ttcattaagt cagatatacc tttatcagaa aatccctttt tctttaattc
    38701 atgtaaatat tctttatcta agtcttctat ttttagaact tttaactttt cttcttgttc
    38761 tactatccat ttaaatttat ttataaacca cttatcaaca ccagttattt ctgatatcat
    38821 ttctacttta tagcctcttc ttatcatttc agctaagtca aatagtctct catcatcagg
    38881 tactactact ctagccttta attcttctaa tgttctacct tctgatggag catgaaccaa
    38941 tgaatatttt cctgtttcaa gtgatcttat acctttaaga atagcagcct caaagttact
    39001 tcctatactc atgatttcac cagttgccat catcttagtt cctaattttc tattggcatg
    39061 tctaaattta tcaaatggcc actttggtat ttttactacc acataatcta atgttggctc
    39121 aaaacaagca taagtttttt tagttactgc attttctatc tcatccaaag tataacctaa
    39181 agctatctta gctgctactt ttgctattgg gtatcctgta gcttttgatg ctagggctga
    39241 tgacctacta actcttggat ttatttctat tatcgcatat tctaaactat gtgggtttaa
    39301 agctatttga acgttacatc caccttgaac ctctattgca tttattatgt ctattgatgc
    39361 ctttctaagt agttgatact cttcatcact tagagtttga cttggagcaa caactatact
    39421 gtctcccgta tgaactccaa ctgggtctac attttccata ttacatacag ttatacagtt
    39481 tccatttcca tccctcatta cttcgtattc tatttctttc caacctttta tactcttttc
    39541 aagaagaact tgtccaacag gacttagctg tagtccatgt gaaagtattt ctcttaattc
    39601 ttcctcagta tcagctatac ctccaccagt tccacctagc gtatatgctg gtcttacaac
    39661 tactggatat cctatcttat tggcaaactc aagacctgct tctaaatttg taactatatc
    39721 actaactatt actggttgat ttatttcttt cattacttct ctaaataaat ctctatcttc
    39781 gccctttttt atagattcta tagatgttcc tataactttt acattatatt tatctaatat
    39841 accagcatca tgtaattcta cagctaagtt taatcctgtc tgaccaccca tacctgcaag
    39901 taaactatct ggtttttctt tttctattat tttttctata aattctattg ttaatggttc
    39961 tatatatatt ttatctgcaa tttctttatc agtcattata gtagctgggt tactatttac
    40021 taatacaact tcaattccct cttcttttaa tgcttggcag gcctgtgttc ctgaatagtc
    40081 aaactctgca gcttgaccta ttattattgg ccctgaccct aatactaaag ttttctttat
    40141 actatctaat ttaggcataa ctctttctcc ttatctaaac aattttcaca gttcttatct
    40201 tcatcatcta tacaatcaac tttaattact ttattttatc ttaaattcaa atttactcat
    40261 caatacatct atttaataca tctatgttaa ttaaaccaaa ctttttgatt taagttattc
    40321 ttgtttttta gttagctatt ctaatgttat ttattttact aattctaaaa atttatcaaa
    40381 aatatagtca ttatcttttg gtcctgggca agcttcagga tggtattgaa cactgtatac
    40441 atctagtttc tcatgtctca ttccttcaac agtattatca tttaaattta tatgtgtcac
    40501 tttcatttgt tcaggaactt ctgaaacata ataaccatga ttttgagaag ttataaatac
    40561 ttttccttct tctaaatctt taactggatg atttccacct ctatgcccaa atttaagttt
    40621 agcagttttt cctccaagag ctaaagctaa aagttgatgt cctaagcata ttccaactat
    40681 aggtttttta ccaataagag cttttatatt ttctataaca tcttctaggt cttctgggtc
    40741 tccaggtcca tttgacaaga atattaagtc agggtttata ctcaatacat cttctggaga
    40801 tgttgtagct ggaaatattg ttatatcaca tccacgagct ataaatgatc ttaatatatt
    40861 tctttttact ccaaaatcca taacagccac ttttggacca tttcccttta tatgctctac
    40921 ttcttttctt gtaacagttc taactgcttc tgtatttgaa aacttatcca atttatgttt
    40981 aacatcttct aatttactgt cttctaatgt aattatacct ttcatggttc cattatttct
    41041 aagtatctta gtcaaagctc ttgtatctat tccttcaagt ccgataactt tattttgctt
    41101 taaatattgg tctatatcaa tttcacatct aaaattactt ggagaatcac ttttctccct
    41161 tactatgaat ccctttactt gaactttttc agactctgct gatgataaat ttataccata
    41221 atttcctaca agaggataag tcatagtaac tatttgacca tagtaagaag ggtctgttaa
    41281 aacttcacca tatcctatca ttgaagtatt aaatactact tctcctacac tttcttctaa
    41341 atatccaaat gcctttccta taaaaactgt tccatcttct aatattaacc ttgctttcat
    41401 actacagtac ctcctataat tctctttaga caaactaaag aacattaact aaatataagc
    41461 ttttaaatat ctatatctat ttttcacttt tacattctag ttttatactt atttacattt
    41521 attttttatc tttttctatt atactacaaa tttcttaaaa aaaagaagtt atctcaaaag
    41581 taaaaactcc tagaaaaaat aacctctttt ttttatttaa taattaaatt cttaaactat
    41641 acaatcaact taatttctat tcagctatat tgtaaacttt taactcatct ttatttaaat
    41701 gtttcttctt aacttctact aaagctttta gtgtatccaa agatgtcatt atttcagtag
    41761 aaaattcaat agctgttctt cttatcttaa atccatctct tgtagagtca tttcctttag
    41821 ttggtgtatt tattactata tcaacttgtt tatttcttat agcatctaat atgtttggtc
    41881 tagattcttc tactctattt actatatcag cctctattcc attttctctt aagctattag
    41941 ctgttccttc tgtagcaaca aaagtatatc ctaattcttt catatctttt gctatctcta
    42001 taaactcatc tttatcatga ttatttacag tagcaagtac tactcctctt tcatcagaca
    42061 tatgtctacc tgctgccaag aaccctttat ataaggcttc ttctaaattt tctcctacac
    42121 ctaggacttc tcctgtagac ttcatttcag gtcctagact tacttcaact ttagataatt
    42181 tagacattga gaatactggt acttttactg atactaattt tggctctttg taaactcctg
    42241 taccatatcc taaatccttt aatttagctc ctaacataca cttagtagcc aagtcaacta
    42301 ttggtactcc acttacttta cttatatacg gaactgttct actagctcta gggttaacct
    42361 caattatata taattcattt tgaaattcta taaactgtat attaaccata ccaagcacat
    42421 taaggtctaa agctattttt ttagtgtatt ctactatctt ttcttttatt tcatcagata
    42481 tattttgact tggatacatt gttgtactat ctccagagtg aactccagct ctttctaaat
    42541 gttccattat accaggtatc aatatatctt ccttatcaca tatagcatct acttctattt
    42601 ctctaccagt tagatattta tctataagta ctggattttt atggtctcta tcaaatgcat
    42661 cttgtaggta ttgagataat ttttcttcgt tataagttat ttccatacct tgaccaccaa
    42721 gtacataaga tggtctaact aatactggat aacctaactc attagcaact tcaatacctt
    42781 cgttaagtga ccatacaccc tttccttttg gtctatttat atctagtctc tctagtaaat
    42841 catcaaattt ttctctatct tctgctgcat ctatatcatc aaaatcagtt cccagtatag
    42901 gtatattttt ttcatgtaaa aatttagcca attttatagc tgtttgtcca ccaaactgta
    42961 gtattacacc ttctggtttt tctttttcta ttatacttaa aacttcttct tctgttaatg
    43021 gttcaaaata cagcttatct gatgtatcaa agtctgtact tacagtttca gggttattgt
    43081 ttatgattat agtttctatg tccatttttc ttaaagattt tacacagtga actgaacaat
    43141 agtcaaattc tataccttga cctattctta ttggaccaga acctaatact atcacctttt
    43201 tcttatcact aacaactacc tcatcatatt gctcataagt tgaataatag taaggagata
    43261 tagcatctat ctcacctgca caagtatcaa ccattttata tgctggtttg atattgtata
    43321 gacttctcaa ttcatataat ttttctggac ttatcttcat taggtcagat atacctttat
    43381 cagagaatcc ctttttcttt aattcaagaa gatagtcttt agttaaatct tcaattttca
    43441 taacttttaa tttttcttct tgctctacta tccatttaaa tttatttata aaccatttat
    43501 ctatacctgt aatttgttct atcatttcta ctttatagcc tcttcttatc atttcagcta
    43561 aatcaaatag tctctcatca tcaggtacta caactctttt ctttaattcc tcaatgcttc
    43621 tctcctcaga tggtgtgtga actaatgaat attttcctgt ttcaagtgat cttatccctt
    43681 taagaatagc agcctcaaaa ttacttccta tactcataat ttcaccagtt gccatcatct
    43741 tagttcctaa ttttctattt gcttttttaa atttatcaaa aggccatttt ggtatttttg
    43801 ccaccacata atctatagtt ggttcaaaac aagcataagt ttttttagtt actgcatttt
    43861 ctatctcatc taaagtataa cctaaagcta tttttgatga aactttagct attggatatc
    43921 ctgtagcttt tgatgctaaa gctgatgacc tactaactct tggatttatc tctataactg
    43981 catattcaaa actatgtgga ttaagtgcaa attgaacgtt acaaccacct tgaacttcta
    44041 ctgcatttaa tatatctaaa gatgcttttt taagcattgc acattctttg ttacttaaag
    44101 tttgagttgg agcaacaact atactgtctc ctgtatgaac tccgactggg tctatatttt
    44161 ccatgttaca tacaactata caatttccct ttgaatctct tattacctca tattctattt
    44221 ctttccaacc ttttatactc ttttcaatta atacttgtcc aacaggactt aattgtagac
    44281 catgtgaaag tatttctctt aattcttcct cattatcagc tataccccca ccagttccac
    44341 ctagtgtgta tgctggtctt acaactactg gataaccaat tgtaagtgca taatcaagac
    44401 ctgcttgtaa atctgtaact atatcactaa ctattacagg ctgatttatt tctttcatta
    44461 cttctctaaa tatatctcta tcttcacctt ttttgataga ttctactgaa gtacctatta
    44521 tttttactcc atatttatct agtatgcctt tttcataaag ttcaactgct aggtttagcc
    44581 ctgtttgtcc acccatacct gcaagtaaac tgtctggtct ttctttttct attatttttt
    44641 ctataaattc tattgttaat ggttctatat atattttatc tgccacttct ttatcagtca
    44701 ttatagtggc tggattacta tttattaata caacttcaat cccttcctct ttcaaagact
    44761 ggcaagcttg tgttcctgaa tagtcaaact ctgcagcttg acctattatt attggtcctg
    44821 accctaatac taaagttttc tttatactat ctaatttagg cataaatttt tctccttatc
    44881 taaatggttt ttactatctg taaataatac taaatctata ttaatgctaa gaatttatta
    44941 aatatacaat ctaaatcttc ttttgttgtg tattctggaa tgtactgaac actatatatt
    45001 ggtaattcct tatgtctcat tccttctata gtattgtcat ttaaatttat gtgagttact
    45061 tctgtatttt ctggcatttc tgaaacatta tatccatgat tttgagaagt tataaatacc
    45121 tttccagttt ctaaacattt aactgggtga ttacctcctc tatgtccaaa tacaagcttt
    45181 gaagtctttc ctcctaatgc caatgctaaa atttgatgtc ctaaacctat tcctgtcaat
    45241 ggctttttgc ctattaattt ttttatattt tcaactatat catttatatc ctctggattt
    45301 cctgggccat tagataataa gattaagtca ggatttatat ttaaaatttc ttctactgta
    45361 gtcattgctg gtactatagt tatatcacag ttacaagcct taagagactc tagtacacta
    45421 tttttaacac caaagtctat aactacaact ttagctccag taccttttat attttcaatt
    45481 tcttttcttg taacattttt tactgaatct ttgttacaat gctcttctaa tttagactga
    45541 acatcttgta atgatacatt ttctaaagtt ataattcctc tcttagttcc attgtttcta
    45601 ataattttag ttaatgctct tgtatctatt ccttcaagtc ctattacctt attttgcttt
    45661 agatatgtgt ctatatccat ttcacatcta aaattatttg ggttatcact tttttctctt
    45721 acaataagtc cttttacttg caccttattt gattcaacat cttctaaatt tattccatag
    45781 tttcctataa gtggataagt catagtaact atttgtccat aataagttgc atctgtcaat
    45841 agttctccat acccagccat tgaagtatta aatacaactt ctccaatagc ttcctttaga
    45901 tatccaaatg cttttccttc aaatactgtt ccatcttcta aaattaattt ccccttcatg
    45961 taagctacct cccaaatatt ttatctaagc attttctatt tctcttacaa tattaatagc
    46021 tgattctctt ggattttctg cttttgttat aggtcttcca acaactaaat aatgagcacc
    46081 tttttttatt gcatctgatg gagttacaac tcttttttgg tctccaacct ctgctgattt
    46141 aggtcttatt cctgggcaaa ctgttataaa atcagttcca catgcttcta ctattttatc
    46201 tacttcttgc gctgaacaaa caaccccatc tattcctgat tcttttgcca gtttagctct
    46261 ctttatagct aactcttctg ttgtcatatt acatcctatg tcctgtaaat cttggttgct
    46321 taatgaagtt aaaactgtta ctccaactac taatggcttt tctatattta atctttctgc
    46381 ttcttcccta gcaattagtg cacattgtct cattccttcc atatctgata catgtattgt
    46441 cattaaccaa acattatctc tcactgcagc tttcactgca cttttcatag tatttggtat
    46501 atcatggaat ttaaggtcta aaaatatttt tttacctttt tctttaagat aatctattgt
    46561 tttacctttt gtggcaacgt attgctctag ccctacctta aatatgtcta cactatcttc
    46621 taatctgtct attagttctt tagctttatc aaattcgtca gtatcaattg caactatcaa
    46681 tttttcttta ccatttatca tttttacatc tcctttttaa taaagcctaa ataatcataa
    46741 aaaaatcctc acaccaaaag aggcataagg attttatcta tacataactc tattcattag
    46801 ctatgagata tctttcccag tctctctgga ctagtttaaa agactttatt atatttaaat
    46861 ttttatttta tactcatcaa aaacctctag aagatttact agaggttata tactaacatt
    46921 atctataacc ctttagtctc tctggactac tttaaggaat ttagctgtaa tatttttctt
    46981 tatttatata actttatgta tctataggat aatttaaagt tttatctttt ttataattaa
    47041 accactaatt gctttttatg tcaataagtc tttgaattat tcttcatctt tatcaaacaa
    47101 ttttttctca aaatatgatt ctgcattata tattgcagca actggattat gcccagttac
    47161 tctatctttg acaactaaag ttgttatagg agcttccata tatttcatgg ctaaagtatc
    47221 gtgaccaaca cataatccaa gaattacatt aaggtcaacc ttagcttcat ttaaaaataa
    47281 agcttgtcct attggattgc acattacctc atctgaacac tttgatattg tttgagatgc
    47341 ttctagtcca attgtactct taggcattgc cccattttta catataacag aaaatacttc
    47401 aaacccatga tgttcaaata ttttttgcac tgtttgcatt tctcttttta gtccaataca
    47461 aaatgctatg ccaatctttt tataaccaca ctttttagca aactctataa tctcttttag
    47521 tctagtatat tgacaatacc cctctgcttc aactaatcca gcattataag ctatttttct
    47581 attctcttct tcttcataaa gttgctttgc tttttgttgt agttcaattt ctctagttgg
    47641 acatatcttc attaatttat ccatatttcc attagaacag cctctaagat tacattttcc
    47701 acacttatac atatccaata tccccttcca aaattttatt catcttgcga ttcttctttt
    47761 tctagagtta cattttcttc ttgggtttct tcttctggtt tcacatgctc gactacagtt
    47821 ataaaatatg attttcctat tttattcctt ataggaagtt tttcatatga aacatctatt
    47881 ttatttttta tacaagctat atcaaaatgt cttaaggcta cacctaagct aaccttattc
    47941 atatcttcac agtgtttctt aggtctcttt tcaaataaat gaagacctcg ttcatcctta
    48001 tggaaatacc acggttgaga attttttaca gatggagctc ttcttgtcaa ttctaagata
    48061 tctttccact ttctattcct atttatctta tttatcatat cttttactgg ctttctatca
    48121 ggtctagcat atctcgtcct aaacaactct tcattttttg ctggatatcc aaatgctatt
    48181 attatataag gattttctat tttgttttca tcatcttctt ctgtttttat tccagctaga
    48241 taatctaaat catcttcctc atcttcttca aaattcttat tcccaatatc tacaaattcc
    48301 aagatatctt ctctctttaa gtttccttcc aaccagcaag tagctattcc caatgttgta
    48361 aggcgcaaaa ctacaccttc tgtagcaaat cctatattct gtaaatagtc ctcacctttg
    48421 tctgatgtaa ctactatata atgaggtgcc tttactttac attccttacc catcaaaaaa
    48481 tgtattatat gacctctatc aacaacatgt gctttaatat ttaaatcttc atttaagtat
    48541 tctaaatttt tacaacttct ttttatttct tccatcaatg gcttttttat cttcttactg
    48601 gaaaaatttc ttacagattt tctataaaaa atggcttcat aaagctccat ttatgccctc
    48661 ctaactgaaa tcttctgacg tataaactct ataaatttat ataaatactt tatatttttt
    48721 atagttttat ttcctttttc ctcctgccct attttgaaat tcataagaat atctactcta
    48781 gtttaatata gcattcaaaa ggcttaaatt ctaccttatc aatgtctata cattagcata
    48841 atttttttat ttgattttct atgctaattt aaataacaac actagctatt acctttttaa
    48901 ttaagctttg ccagaaaaat tttttttcat ttataaatat ctatctcatt attatattat
    48961 aagttttttt cggtattctt gtatagcttt tcactaaaaa agtattttta tttctacttt
    49021 aatattatta tcaacaaaaa acatactgct taaataagtt tgttatcctc ataatttgtt
    49081 ttaattaaaa tatttttttt atttctcaat ctataaagta taaatatttt gaatatgacc
    49141 caaaatctaa gtaaagttat aatattttat aagttgttca atattaaaaa cataagtaat
    49201 gttataatac tttataagtt attcaatatt aaaaatatag gaggataaat atgaatttaa
    49261 atagaaattt agaaaaatac gctgaattag caataaaagt tggagttaac atacagccag
    49321 gtcaaattct acttataaag tcaccaatag aatgtgctga ttttgcaaga aatgcactta
    49381 aagaagcata taaatgtggt gctaaaaatg tttatataga gtggtctgat gaagaaagta
    49441 ctcttattaa atacttatat gctccagatg aagcatttca tgagttcccc aaatggacag
    49501 cagaacaata tgtagatata gcaaaagaag gtggggcatt tttatcagtc tatgcccaaa
    49561 atccagattt attaaaagat gttgaccctg aaagagttgc aaattttcag aaggcatctg
    49621 gaaaagcttt aaaagaatgg cgttcttaca ctctaactga taaatgtaaa tggaatatag
    49681 tctctgtgcc aacaaaagat tgggcaaaaa aagtgtttcc aaatttatca gaagataaag
    49741 caatagaaaa actgtgggat gctatattta aatgttcaag agtagatggt caggacccta
    49801 taaaagcatg ggaagaacat aatgaaaatc ttaaatctaa aatggacttt ctaaataaaa
    49861 ataactttaa aactttaaaa tataaatcct caaaaactga tttgactcta gacttgccaa
    49921 agggtcatgt ttggctaagt ggagcatcca aagaccctaa tggaataagt tttaatccga
    49981 atattccaac tgaagagatt tttggaatgc cacataagtt taaagtaaat ggaactgttt
    50041 atagtacaaa acctcttgtt tatggtggaa acataattga caatttcttc ttaacattta
    50101 aagatggtaa aataatagat ttctctgctg aaaagggact agatacacta gaaaaactta
    50161 ttgaaacgga tgaaggctct cattatttag gagaagtcgc tcttgttcca tacaactcac
    50221 caatatccaa tacggatata attttttata atactctata tgatgaaaat gcttcttgcc
    50281 actttgcaat tggttctgct tataagactt gtctggaagg tggaaataat ttaaaagatg
    50341 aagaattgga tgaacatggt gtaaatgaca gtttaacaca tgtagatttt atgattggtt
    50401 ctgctgatat ggatattatt ggtgagactt atgatggaaa gcaaatacag atttttaaaa
    50461 atggtaattg ggctttttaa catatgattt taactaaaaa gggctgatgg aagttatacc
    50521 agacttccca gcccttttta atgttgatga taaatatttt ttatattaat taatctcatt
    50581 attatagatt aattcctatt gttttaaaca taagtatcta aaacttatat ttatattata
    50641 tataaaatat cctatttagt aaaaccttta ttttaattta tatgctaaaa atttaacttt
    50701 gctatttcaa cagcagactt tgcatccttt gaataataat cagcacccat ttcttcagcg
    50761 tattcttctg tcaaaactgc acctcctaca aatacctttg catttatctc attatctctc
    50821 agtgcctgaa ttgtatcttt catactttga acagtcgtag tcataagtgc acttaatcct
    50881 actaatttta tatttctctt tttagcaact tccactactt cttcaatagg gacatcttta
    50941 cctaaatcta taacttcata tccataattt tctagcatta tttttactat atttttccca
    51001 atatcatgga tatcaccttt tactgtagca actattattt ttcctttgga tacattattg
    51061 ctacttttac ttaataaagt ttcctttatt gtatttaatg aaacttttac cgtttctgct
    51121 gattgaatca attgtggtaa aaatatatct ccactatcat atctttttcc tactttatca
    51181 agagcaggaa ttaaaatctc atctaatata taattttcat ccttctcttt taaaagattc
    51241 agtgttacat tttttgcttc atcttttaat cctctttcaa ctaaaatatc caagggcaaa
    51301 tctttatcaa tctttgtaga tgaatccgat ttttttgaat tatttatatt gctatattga
    51361 tttatatagt ttatacatcc tttatctgtg ttatttaata ttttaaaaga attgatagct
    51421 tccatcatac tatattcatt tggatttata attgctaaat caagccctgc ccctaaagct
    51481 aatgttagaa aagaagcatt aagtgctttc ctatttggaa gaccaaatga aatatttgaa
    51541 acacctaata tagtttttac tcctagtgtc tttaccattg ttatagcttt tatagtttct
    51601 attgcttctt cttgttgtgc tgaaacagtt aatgataaac agtctataaa tatatctttt
    51661 ggttttatcc cataagaaac tgctctatta actatccttt ttgctatttc aaatctttct
    51721 tcagcctttt taggtatccc cttttcatca agtgtaagtc caaccacaca tgaaccatac
    51781 tttttaacta ttggtaatat actttctaaa gactcctctt tgccatttac agagtttact
    51841 attgttctac cattataata tctaagtcct tgctctaagg cctctacatt ggaagaatca
    51901 acttgtaaag gtgtatctac tactgcttga atctctctta ttaattttgg cattagtttt
    51961 ttttcatcaa tctctggaag accaacattt actccaagta tttgtgctcc agcatttact
    52021 tgctctaaac ctaaatttat agcataatca acattttcat tttttaatgc ttcttgtaga
    52081 gattttctac ctgtgggatt caatctttct ccaacaactg taggcgattg tacttccaca
    52141 aattttgatg gtgaacagac tacacattta tcaattttct cgatacatcc ctttgtaaca
    52201 gaatttatat tctcagatat ttcttttata aaacttggat tcgtaccaca acatccacct
    52261 attatagtag caccaacttc aacgaacttt ttaacccctt caaaaaattc ttttgcatcg
    52321 acatcatata tagcctctcc atcaactata tttggaagac ctgcatttgc ttgaaccata
    52381 acagggactg tagctcttga tgctattttt tcaactattg gtaaaagttg tttaggtcct
    52441 aatgaacaat taactcctat tgcatctaca cctagacctt ctatagtagc caccatacat
    52501 tctggcatac atccagtaaa acttcttcca ccttcatcaa atgtcatagt acagaaaata
    52561 ggtaaatcag agttttcttt tgctgctaat acagctactt tagcttcata taaatccatc
    52621 atagtctcta ttactattac atcaactcca gatttaacgc cttgtagtac ttgtctttta
    52681 aatatttcat atgctttatc aaaacttaaa gtacccatag gctctagcat ttctcctata
    52741 ggaccaatat ctaaagctac atatcttggt ttagtattat ctacattttc tatagctttt
    52801 ctagccacca aaacagcatt gtcaatcact tcttctacag tatacttact atctaatttt
    52861 agttcattac atccaaaagt atttgtaaga attacattcg aacctgcctc taagtatgct
    52921 gtatgtattt ctataagttt atctggattt tgaagaccaa atacttctgg attttcacct
    52981 aactttaacc ctttttgttg aagcattgtt cccattgcac catcaaatat taatatatta
    53041 tttttcagat attgtcttat ttccacaaga gttcgctccc tttctatatg tacagtttct
    53101 atagttttta caattttcac acgacttttc tttcttagat ttatttattc ttgtattaga
    53161 aatacctata atagccacta ctgactttct cggtatcata attccattag aagtaattgt
    53221 tagacctatt tgtttttgag cattcaaaac gtttaatata tctatatttt tttctattgg
    53281 taaatctcca tatcctgggc tatatcgcat agttataaat ttatcttgct ttaaaaaaac
    53341 cttttctaaa ttattttgaa caatgtcaca cacttcttct attgctgttg ttccacatga
    53401 atcaattatt agtccttttg ttaaatttga ataagagtgt cgtcttattt ctttttctat
    53461 atctatccct atagtagctg ccataattac acactcatta cattctttta aaagttcata
    53521 tacatattta ctctctaatg ttaaatttgt tccctctaat tctacaacat tatctttatt
    53581 tattctaata ggataaatcc tagataaata tctaggattt attttatttt tagttatagc
    53641 tacacattca tcaatagtat atttaaggtc tttatctatc tcttgacctc tgtattgtaa
    53701 atatcttaaa acttcacttt gatttatgct tatatcttct cttccaatta aattattctc
    53761 catatatatt taaagaaaat cctttatcta aaacatcttt tgcatattcc tttgccccaa
    53821 ataaagataa gtctctgtag tttccacatt ctatagctgt agctgctggc attttatctg
    53881 attcaagaac tttttttaat gcttcttcta aaccaatctt aactgttttg gcatctacat
    53941 ctccccatag tattaaataa aatcctgttc tacatcccat tggagataag tctattacac
    54001 catctaatgt atctcttaaa taagtagcta aaagatgctc taaaccgtgc atagcagcag
    54061 ttccaaaact ttctacattt ggctgtaaaa atctcaagtc aaatttagtt actttatctc
    54121 cctttactcc atcaagtaca ctacactttc ttacaaaagg agcttttact tttgtatggt
    54181 ctagcttaaa actttctact ttttccatta tttaatcctc cattatactt taaaatttgt
    54241 tacattaatt ctaaaaaaaa ctcttcctat ctattattga taagaagagt atattatttc
    54301 aagtcatctc atcgttgtta gcaggaccac ctgataaaag gttggtatag gatcactgag
    54361 ctaactctct cccctaactc ttgataagta tcttattaaa ctgacttaaa ttatatatta
    54421 cttttttatt tttgtaaaca ctttattgtt ataatattta tatcacataa tcgtactttt
    54481 attttataac taagctgatt gtattattgg cagttgtact tcaaacacag tttgatattt
    54541 atcgcttgaa acagtaattt ctccatcatg taaatttaca atattttttg taatcgcaag
    54601 acccaatcca gaacctccaa catttctatt tctcgatttt tcaactctat aaaatctatc
    54661 aaatatattt ggtaaatcta acactggaat tggttcccca taatttatca cttttacaac
    54721 cgccatatct tcctttttct ctgtgactat atctacataa tgcccatctt ttccatattt
    54781 cacagcatta gttataagat tttcaaatgc tcttaccaat ttatttggat cagcttttac
    54841 aattaatttt tcttcattaa aattaattct actaaccata aattcttgtt taaaataaat
    54901 ttcaaattga gatactactt gacttaataa ctctactaaa tttatttcta ttttgtttaa
    54961 ttttattgta ttattttgca ttttagtcaa ttcaaacaaa tcatttataa gaacatttaa
    55021 atttttcgat ttttcatatg caatatcaac atagtacatc atctgaactt catctttata
    55081 cttaccttct tctatcactc ctaaataacc cattatggat gtcaaaggtg ttcttaagtc
    55141 atgtgacaca tttgttatta aatctgtttt tgtctgctga gcttgtctct cttcaatagt
    55201 tatatttcta agttgatgga ctatattatt gattttatgt gctagttcat caatatcatc
    55261 cttttcttta tcctttaata tatctaaatc atactcaata tctatacttt tttctaagtt
    55321 accttcatcc attaaattta cagcactttt aatgatttct aatttttgat tttttctatt
    55381 agtctttctt ataaaatcta caatttgctt atttctcttg tacgatataa tggaataaat
    55441 tgctaatgat gtgattgtaa ataaaaatgg ataaaataaa tcatacccta taagacttct
    55501 gaaatctctt gcttgatata caagttgatt aactacagac atatcactat tgacatataa
    55561 tgattttatt atatacatac ttgttaaaat aagcattgaa gttaaagcac agcttattat
    55621 aagatctaat atgggtcgaa acatatgatt taataaactt ttatttttca attttatatc
    55681 ctactcccca tacagtatga attatttttt ctccatctgt atatttttca attttatccc
    55741 ttattctgct catatgaacc attacagtat tattagattg gtaatatttc tctttccata
    55801 ccttttcaaa tatttcttct gtactatata ctctgccttt attatttgct agtaaatata
    55861 atatatcaaa ttctcttgat gtcaaaatta tttcatttcc agccactgtt acactatgtt
    55921 tttccttatc tataattact actccaacct ttatgacatc tttatcttca ttattagcac
    55981 cactaaaata atatgctctt ctaatcagcg acttcactct cacagtaagt tcaagtgggt
    56041 taaatggttt tgtcaaatag tcatctgcac cagtcattat cccttgtatt ttatccatgt
    56101 cttctgattt tgcacttaac ataagtattg gtatattcaa actttctcta acttttctac
    56161 aaacttctat cccatccatt ttaggcatca ttatatccaa tatcattaaa tgaatatttt
    56221 ccttttttaa aatatctaga gcctcttttc catcttcagc tttaattaca ttatatccac
    56281 tatttaaaag atatatctct ataaggtctc ttatttcctt ttcatcatcc acaacaagaa
    56341 ctgtattatt catttttaca catcctaact taatctttgt atatagcttt attcatataa
    56401 aattatacta tattttagtt agtgtaatct ataaatttat taatttctaa atttccaata
    56461 taatacaaat tatttacaca tttatattat ttattttgta aatattcttc taaagttaat
    56521 ccttttgaat aaatttcctc agcagcatcc ttaccaacat atctaatatg ccatggttca
    56581 tatgctgtac ctgttatatc ttctttacct tcaggatatc ttattataaa tccaaacttg
    56641 tgagcatttt ctgccaacca tttagcttct tttgtacttc caacaaacca tctttctgga
    56701 tttgttaaat caatacaaag tccagtttgg tgctcactgt aaccaggctt tgctacatat
    56761 gcatctgcct tttctcgccc ctgagatttt acccttcttg tataagtatc cagttgagta
    56821 tcataagatc tgtatgctga agaccctaaa aactgtattc cttctgaatt agcttgctta
    56881 actaaatctt caactgcctt tgcaggttct cctgccattt gtttttcttc ctctgttgct
    56941 tcttcaacaa aaggtatgtt taccttagta atattttcag gagtatagtt tttgctaatt
    57001 ccatttgtct tgtttaccaa taatatattt gatatactat ttgagtgatt tttattttga
    57061 gaatttgcct caacatcttg ttctgaatca tgttgagcag ctactattgg atctttatct
    57121 ttcttaagta atctatcttt agatataaat acacttataa atactactaa aataatggct
    57181 cctattaatt taaatttttt cataattaaa tcttctcctt tttgttatgt ttgctataat
    57241 gttaatttta gagtataaga cttaagtatt ttataagtaa atcttaaata aatcttaaat
    57301 ttaatatttt taaaggatgg tgataaaatg tctatacaaa aacttctaag taaagctcgt
    57361 aaggctatac aagattttga tatgatacaa gaaaatgata aaattgcggt tggactttct
    57421 ggtggaaaag atagtttaac tctacttcat atacttaaaa gttatcaaag attttctcca
    57481 caaaactttg aattgatagc cataacatta aatactggag gtgttgataa ttctccacta
    57541 gataaattgt gtaaagaaat aaatgttcct ttttatgaat ttcaaactga cataaaagaa
    57601 attgtatttg atataagaca agaaaagaat ccttgtgcat tgtgtgctaa tttaagaaga
    57661 ggtgctttga acgataatgc taaaaaactt ggatgtaata aagtagctct tggtcatcat
    57721 aaagatgatg caattgagac atttttaatg tctatgtttt atgagggtag agtaaattgt
    57781 ttttccccaa agacatacct agacagacag gatttaacag ttataagacc tatggtttac
    57841 atagaagagt atatgactaa gaaaatttca aaagattcta actatcctat tatcacaaat
    57901 ccctgccctg caaatggtca tactaggaga gagtacataa aaaatcttat tgctaattta
    57961 aataaagaga tgcctgattt taaaagaaat gtatttggtg ctttaaataa ttctgagaaa
    58021 ttgtttatat gggataaaga gaagataaaa aattttaaat aataattaaa atttttgaat
    58081 aaaaaagcaa gattgtatta attgtaaata atctttatat aatctacaca atcttacttt
    58141 tttatttatt atatgttatt atctttaatc tatattttaa tagttcttaa tagtatttat
    58201 tttgtctaat acattaatta taacttaaat attttatata ttaatttaat ctattaattc
    58261 attcaatcta ctggtttaat ccatttttac agtatataca tctacatatt ataaaaagca
    58321 aaattatatt ttctaaatat tttatgttag attataatat taaattctat tatctctggt
    58381 atattaaaaa atctaaatgg taactttgta cttccaagac ccgaactcac atataaatga
    58441 tttccattta tatcataaaa ccctttagta tatttccttc catatggagg agtaactaat
    58501 gctcccttaa taggtaatct tatttgacca ccatgactat gcccagatag aactaaattc
    58561 attgtatctt tactaagcat atctactaaa tctggttcat gtgctaacaa taaattaaaa
    58621 ttcttattat ctatttgact ctccagatat tttatattag gatttccatt taatatttca
    58681 tcaacaccta atatattaat atatttatca tcttttaatt ttattttttt attttcattt
    58741 actaaaagat tgaagttgga atttttcata atctgcctat aatacctagg aagtttatac
    58801 atataatcat gattgccaaa tactgaaaat tttcccaacc tagcattcat acttgataaa
    58861 atagttgtaa tttcatctac acttggattt ttattggagt aatcaattaa atcaccagta
    58921 aatactatta tatctggatt caaagtgtta atcttattag ctactttttt gagattctta
    58981 gttgaataaa aactacctat ttgagtatct gaaatttgaa ctacttttat cctttcagat
    59041 ttactctcaa ttgtatcact ttttatcaat gttccatttt cacttatatt atattttttc
    59101 acaattaata gactcggctc tatcgcaact gagtatataa aaatcaaaat aaatattaag
    59161 ataaaaaatt ttaaaaaata cttaattttt gagttcaaaa tatctaccta cttatttatt
    59221 tttttaaact atttgcatta cagactcaac tttgttcatt tttctgtcat gcatttttgg
    59281 cttatttgct gaaaaccatt tatatgttgc tgtaagacct tcttttagta acacatttgg
    59341 tatataaagc ccatgttcaa taagcttatt tatatcaaga ttaaagtcta tatttctaaa
    59401 tgggaaatac gttctttctc taaactctac tttttccata tctacatatt ttattatagg
    59461 ttctttgcca ataatttctc cacatgtata tataaggtca tcccaagaaa ttaactgtgg
    59521 atttgtcaca ttataggcct ctcttacatg aggatttttc attatgcttt ccaatacctt
    59581 taccaaatcc ccaatatata taaattgatt actaacttgt tttccttttg gtactggtat
    59641 cttttcattt ttttctattt tttcaaagaa ataatactct ctatataaat tattattttc
    59701 accatatata taagttggtc taataattat atatggtata gggctattta taataaaatc
    59761 ttcagcctct tttttattta acccatattt tccccagttt tcattttcac ctttctcatt
    59821 ttcttctttt atatttcttc cactatcttt atatactgct cctgctgaca atactatata
    59881 cttttttaag ttatccatac ttataaaatc aatcagatta cttacatctt cttttgtata
    59941 tgctgtcata tcatatatgt aatcatattt tcttcctgtg ataatgtttt ccatatcttt
    60001 tcgaactttt ctatcacaaa ttagatgttc tttaaaacca ttataatcta tttccttttt
    60061 accatttgta agtatatcaa tttgataccc acatttaatc aagcgcttag caagtgcaga
    60121 acctatgaag tcgcttccac ccataattaa aatagatttc attttaagtg catcctcctc
    60181 ttttttaaat gacttcctta actataatat catattttta aatatgcgac accattttgt
    60241 aatatttttg tctataaaag atatttataa caaaatatct tttatagact ctacattttc
    60301 gacaatataa gtaggttcac tttcactaag ttcttcaaaa gaaccatacc catacaacac
    60361 tccaatagaa tctataccta tttttttggc tccaattatg tcatactttc tatctccaac
    60421 cataataact ttatctttat cttttacatt acataggtct agtacatatt gtataacttc
    60481 atttttattc actcttgtac catcaagatt gctacctgct atatatttaa aatatctatc
    60541 tatatcaaaa tatctaagta tagtttctgc aaaaactgtt ggcttagaag ttgctactaa
    60601 taatatttta ccatttttat agagcatttc taatatctct tttatattct catagatttt
    60661 attttcaaat atccctttat cagcaaaata ttctctatac ttttcaacag cttctttagt
    60721 ttttttatct tcaaatttat aatactcctt aaaagtatca tgtaatggtg gtcctataaa
    60781 ttggtctaag ttttccaaat cttcttttat gccaaagcta tttaaagaat actgaataga
    60841 ctttgttatt ccttctttag ggtctgtaag tgttccatct aaatcgaata atactatttc
    60901 ataatttttt ttcatatttc agtcctccat atttgtaaaa agtaatatct tatatattaa
    60961 gtttatctat tttaaaaagc tatagtaata gaaatttcta atactatagc ttaagtaccc
    61021 aattaaatct tatatattta ttttaaaaat aaatttaaac agctctattt aactgttata
    61081 gctgaaactg gacatccttc ttcggcttct ttacataaat cttgtaagtc tgctggtatt
    61141 tctccttcaa ttgctactga atgcccttca tcgctcatac taaaaacgtc tggacatgtt
    61201 cctatacatg ctccacaagc tacacataca tccttatcaa caaatgcttt catactaaaa
    61261 tacctcccct aaaattatta ttttttaatt gttgtttcct tagatattta tccaatatat
    61321 accaatttaa acatatattt attattttat cactattgtt tagtttatta tagtattttc
    61381 gtatccatgt ctataaactt cactgctttt catccatgat tttacagtat attgcaatct
    61441 taatttttta tttaaaacaa gttcattatt attgtagtct tttataatat cattacactc
    61501 ctccattgtg aatattgtaa aatcaccttc tccaccaatc tttattctgc ctttatttcc
    61561 atgtaaatcg aaaatctttg ttgggtattc agttactttt tctatacact tttctaagga
    61621 tatcccaagt tccataattt tatttataac acttaaaaga gaatagttta cgtctttcat
    61681 gttttttata tgcatatcag tacttattag gtctggctct aaaccttgct ttattgcttt
    61741 tttaaaaact ttaaaatcaa attggttcaa tccatgacat acatcaaaca atactcctct
    61801 gttctttgca tttattactg attttcttaa tgtgccagat tctttaagga ttccatttgt
    61861 actatttcca tatactggtg taaccacatc tccttttccc aaaatattca ttacttcatc
    61921 tatatatgat ggatactctc ctatatatac cactaagggt aaatttaatt ttttggcaac
    61981 ttgctttgat ttcttaatta tatctatacc actttcatta gattgaaatt tactcgcttt
    62041 tacttttata ccctttatct tttctatatt tttttctaca actgactcaa ccttgtcaaa
    62101 atttataaca tctaaagaag ttgattcatt caatatattt agtccctcat ttgtaacatt
    62161 taaaaaagaa tatacttctg ttttacactc atccatatac ttttttttaa aatcatagta
    62221 gtttaaagga ccagctgtac ctgcatcaaa tataactgta gaaccttttt caattccaat
    62281 tgaatcagaa tccatacctg taggtaattt tctcttataa caatgggtgt gaacatctat
    62341 aaatcctggg gaaatataca tattatcggc atttattatc tcgaactctt tatttgtaag
    62401 taatttttct tttcttattt tctctttaag gttcaatcca atagcatata ttttcccatg
    62461 taaaaccaag atatctcttt tttcaaattt gataccatct gttggagata ttattgtccc
    62521 atttcttata agcaaatttt tcatttattc cacatcctaa ttacaatata atttctctaa
    62581 tagcttttct tacataaaga aaataagcct aaaaataaaa atattttaga cttattcact
    62641 atattatact atattaatta tattaaatta aataaaatct ttaatttact ttaagaagca
    62701 attaagtatc tgttgttcgg cttcttcttc agtaagacct aatgtcatca tctttagtat
    62761 ttgatctcct gcaatctttc caatagctgc ttcatgtact aaaagagcat ctggatgatt
    62821 tgcagcaatt tcaggtatag cactaatttg agaatttccc ataattattg aatcacattg
    62881 aacatgtgca gaacaaaggg aatccccaat aacacaagga ttaaactttt gcttagaatt
    62941 atcttgccca actgtacgag aaactacttg agcagaagag ttttccccct ctaagataac
    63001 cttcatatta gattctgcaa cttgattacc atgagttaat aatttctcaa atacaactat
    63061 tttagctcct ttacctaggt ttgcatccat ttctctttta gttgaatcta cacccttaat
    63121 ttgaattgtt tccatttcac aatatgaatc ctcatccata ttgacaatag taactggatt
    63181 taaaattctc tttccttctc catcaccttc accataatgt ttttctacat attttaggcg
    63241 agctccttta cgtaagaaaa atctatgtat accattatgt tcactatcat cacaaccaca
    63301 gttatgtata ccacacccag caattataac aacatctgca ttttctccaa tttcaaaatc
    63361 attatatacc atatcctgta caccagtctc tgttactatt actggtatat gtacattttc
    63421 tccttttgta tttggcttta ctataatatc tattccaggt ttatcagttt taggaattat
    63481 agttacattt tcagttgaat gtctctctac tccttgccca tttttacgta tattgtatgc
    63541 tcctaatgga gttccacgaa gttcagcaac tttttctaaa agatttaaat ctattttgtt
    63601 catttaattt gccttccttt ctaattaatt catgtctttc attaattcac aacttgaatt
    63661 aacttgacac atgatttcag gtaatatagc ctcttttgtt ccatggcttt ttactgaacc
    63721 atcttgtaga acaattattt catcagctaa ctctaatatt ctttcttggt gagaaattat
    63781 gattatagtc tggttagact cttcatgtat tttcttaaaa ctttcattga gtttcccaaa
    63841 gctccaaagg tctatccctg cttcaggttc atcaaatata gaaaccttta aatctctagc
    63901 aaaaagagtt gctatctcaa ttcttttcat ttcccctcca gaaagagagt tatcaacttc
    63961 acgatttaaa tagtcttttg aacacaatcc aacatctgta agatattggc aacatacatc
    64021 agtagacaat ggttttccat gagcaagtgt aagtaaattt tcaactgtca taccttttat
    64081 tcttggtggt tgttgaaatg catatcctat tccttttttt gctctttctg taatactaag
    64141 atttgttata tcttctccat ctaaaattat ctgaccactc gtagcttttt caattcccat
    64201 aataagctta gcaattgtag attttcctcc accatttggc cctgtgataa caataagttt
    64261 tcccctctca aatgaaaggc taacatcatt tattattcct agttcctcat tgttagactc
    64321 aacattaaag gaaagatttt ttatttcaag cataataccc ctcttttcta taaaatatta
    64381 cattaatatc cattctttta ttatatcagt aatttttata tatcagcaac tctaagcaga
    64441 taaaatgtat attaaataat aaaacaaagt tattgaaaag taaaaaagat gagttttatg
    64501 taaactcatc tcagaaattt agtatatcaa cttatatgta tttaaaaatt agccttctat
    64561 atactctaat attatttttt gattatcttt taacataatt tcttcttttt tagattctat
    64621 tccattcttt tttatcttca cctcagctac aaaggcatca ttaccttcaa ccatttctaa
    64681 tagttcataa gcttttttac gacctatttt agttgctata tcttttagta ctgccatttt
    64741 cttaaacatt ttatcacctc tactcaaaat ttacacttaa tttttaatta tataattcta
    64801 atcctagact agtttataat aattattgtc tctaattata tcagatattg agtaaattta
    64861 atactttgta aaattattta tcctctctga ccaaataatc atttccatct tggtctttaa
    64921 aagaaaacat ccttccatat ggcatctcca ttaattcccc aacctcaacc tcgttatttg
    64981 ccatttgttc ataagttcca ttaatatctc ttgtacttaa aattattgat ggatgagata
    65041 catttatctc tttgttttgt ctcatcatta gatttttatc atacaacaca aatgttgtaa
    65101 attcattttt acttggtgct acttccaccc atttcatatt tggacccatt tccttttcat
    65161 aagttactac aaagttcaat ttttctgtcc aaaacaactt tgcatcctct tgattattta
    65221 catagagagt tatttttcct attttattta tcataacaat acccaccttt attattaaat
    65281 aaatacctaa tattttttat atttaataat atacccatat atattatttt ctatagatat
    65341 atatttctaa tatttatcgc caaattatgt aattctgctc aatatataat ttatagtatc
    65401 catactatta taggctttat ttatgtaatt taatcacctt atatatcata cttataatta
    65461 tgataataac tacataaaaa atcacttcta aatagagtta tccatctaaa agtgatttca
    65521 tatattctat gcaactttgt gttttagcca ctttgttcct cttaatctta aacaatataa
    65581 agtccctcta acaagccaat cagtatacat acctatccaa attcctaaca ctccaaattc
    65641 taatacaacc cccaatacgt aacctgtaaa aattctaaaa atccacatac ctattactgc
    65701 agtcatcatt gtatatctag tatcaccggc tccctttagc cctgctgata atacaaagga
    65761 tataggccat attaccatag ctaaacaatc acttcgaatt aattgtgttg agatgtcaat
    65821 tacactagca ttttgagtat acatacttga aatccaaggt gcaattggta taaatatagc
    65881 tcctactata aataaacaaa ttgttgcaaa tttagttaaa tataataaag tactcttagc
    65941 tcctttaata tctccacgtc ctatatactg tcccaccaag gttgtagcag acaaagctaa
    66001 agcattccca attacattta ttatggatgc aattgacatt cctattgcat tggatgcaat
    66061 tgctgatgtt cccatagtaa ctataaatac ttgaacaatc aatttacctg cattaaaaag
    66121 tagttgttca actccagctg gtattcctat attaaatata gacttttgag tttcaacatc
    66181 aatcttaaat ggaaatattt tacccataga tataaattcg tttcctttaa ataaagctct
    66241 tataattact atacatccaa ttattctagc tatagcaata gatatagctg cacctgttat
    66301 gccataggat ggtgtttgca aatttattcc tagaggattt aaatcaatac catatataag
    66361 tatatatcct aatgttacat ttactatatt cataaacata gtcacataca taggtttctt
    66421 tacatctcca caccctctta aaagaccatt tgctatttgt tgtgtcgcaa taaatggata
    66481 cgtaaataat gtaaactcta tgtataattt tgcatttgat tttaccaatt cttctgcgga
    66541 accataaaat aaattaatta caccagttct aaaaacccac atcaatattg ttattgtaaa
    66601 ggataatatt actccagaca ctagagcctg cttaatagtt tcattcacct tttcaccatc
    66661 tttttttcct atttgttgag ctacaacaac agtagctcca acagatagtg cagcaaatac
    66721 agatataaat atattgttta ttgaatcaac cattccaact gcagatacag cttcttcccc
    66781 aatatgacct gccatcattg tattaaatac tcctaaaagc atgacaaacg tctgctctat
    66841 cattatagga attgctaact ttataacatt tttccttata aacatcccta ttcctctttt
    66901 ccctctttat ataaatttaa tttttaatta gtttatcgaa ctgtaaattg acaatgcaaa
    66961 gtttagaatt gtattgttcg cattataata tactgctttc aaataatata aatcttgttt
    67021 aatatcattt gaacacagta tatctttata attatcttat gtttattatt ttttatggat
    67081 accacattta aaatatttat taatttaata taaatatttt ccgttattat taaaagtagc
    67141 tatatttcgt taatattgta tcactatcgt gtagcaataa caataagtac attttatata
    67201 ttttttataa tattatttaa tcatccaaac ttttaccact ttttcaatat ataaaatatt
    67261 acactttaga tgtaacaaaa tcgcatccat ttaaatattg tattttatag actaatttta
    67321 ggatgtgata ctttggttaa tttaaatgca aataatctaa atgtaaatat ggtttatcct
    67381 atttcttcca cagcacccat acgtttcaga agatatactg tttcatattt agaaaacatg
    67441 gaaaatattt atttaactat agcaacatac tttgatacac ttggtttagg cacaatgaat
    67501 atcgacaaag tttatggtca atgggcttgg tttactgata atatatatga tttaaatcta
    67561 tttgtttttg taggaaatta tccttatcaa attgctaaaa acagatatga tacatttgta
    67621 gatattttgc cagtagctat tacttctatt gtcaatggag atagaatatt tttaatttca
    67681 aatccttgtt tgttaaattc aaaaattaca gttagattta tatctagtca tcctactctt
    67741 aatagaacag ttgattatgg caatattaga gattttattt tttaaaactg caacatgatg
    67801 tgctattaaa aaatacccta aatttaatga tattattaaa taactagaat atttttaatt
    67861 ggcacatcat gcctagctat tataaattca taatattatt ttacttaaga taagtactgt
    67921 attttatcta cttgtatatt tttatttacc tagtttctca gttctatatc ctatttcttt
    67981 attagctagc ttataacctt cttgtatcat ttcttctctc gttaatgcac tccctaataa
    68041 attgtcaata ctcagttgct ttctatcact aaaatactta tccaataatg attcctctat
    68101 atcttcctga tatgccaaat ttccttcatg cattatatat tttacatcaa aaccttcttt
    68161 atttaatact tttcccacca agatacttct atgacatctt ataggttctt gcatagctcc
    68221 taataaaact atcctataac ccatttgaca accctttttt aatctttcta ttccgtttaa
    68281 aaaaatatct tcttttgcta ctttctcaaa atctgcataa ccttcttgcg tatatacatc
    68341 tttattagtt cttttagctc caaattcttt tcccatgtat atatataaaa atccggaatt
    68401 tctaagagat tcgttaaatg cctctttatt gtattgaata ttatatttag aatatggagt
    68461 ttctctaatg tcaactacac aatttatgtc atattttttt atcatattaa taagcttatc
    68521 atatggataa tttgaatgtc ctattgcgaa tatttccata taaacaccct cctattcttt
    68581 gaaattagta tctatatatg tagatacccg atttacttaa aattaaataa agtattactt
    68641 taaataccca atatcttttt ttcttgatat tgtatatttt tttactt
//
LOCUS       DBGJFU010000005        66101 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.005, whole genome shotgun sequence.
ACCESSION   DBGJFU010000005 DBGJFU010000000
VERSION     DBGJFU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 66101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.005"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(404..925)
                     /locus_tag="ACOIAS_000332"
     CDS             complement(404..925)
                     /locus_tag="ACOIAS_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3072 family TudS-related putative desulfidase"
                     /protein_id="HGZ2700063.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            1091..1657
                     /locus_tag="ACOIAS_000333"
     CDS             1091..1657
                     /locus_tag="ACOIAS_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3073 family putative ECF transporter S
                     component"
                     /protein_id="HGZ2700064.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTIGTVLGAVV
                     LGPFWSALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(1874..3937)
                     /locus_tag="ACOIAS_000334"
     CDS             complement(1874..3937)
                     /locus_tag="ACOIAS_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HGZ2700065.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(4318..7653)
                     /locus_tag="ACOIAS_000335"
     CDS             complement(4318..7653)
                     /locus_tag="ACOIAS_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGZ2700066.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(7703..8131)
                     /locus_tag="ACOIAS_000336"
     CDS             complement(7703..8131)
                     /locus_tag="ACOIAS_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700067.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(8121..8501)
                     /locus_tag="ACOIAS_000337"
     CDS             complement(8121..8501)
                     /locus_tag="ACOIAS_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGZ2700068.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYKSKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(8534..8842)
                     /locus_tag="ACOIAS_000338"
     CDS             complement(8534..8842)
                     /locus_tag="ACOIAS_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700069.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(8887..10047)
                     /locus_tag="ACOIAS_000339"
     CDS             complement(8887..10047)
                     /locus_tag="ACOIAS_000339"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGZ2700070.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISK
                     VLDKAQELIDKRKEQIEALDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKG"
     gene            complement(10051..11514)
                     /locus_tag="ACOIAS_000340"
     CDS             complement(10051..11514)
                     /locus_tag="ACOIAS_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGZ2700071.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            complement(12023..12892)
                     /gene="gatY"
                     /locus_tag="ACOIAS_000341"
     CDS             complement(12023..12892)
                     /gene="gatY"
                     /locus_tag="ACOIAS_000341"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGZ2700072.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(12912..14828)
                     /locus_tag="ACOIAS_000342"
     CDS             complement(12912..14828)
                     /locus_tag="ACOIAS_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGZ2700073.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGL
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(14831..15751)
                     /locus_tag="ACOIAS_000343"
     CDS             complement(14831..15751)
                     /locus_tag="ACOIAS_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HGZ2700074.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICENKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(15853..16605)
                     /locus_tag="ACOIAS_000344"
     CDS             complement(15853..16605)
                     /locus_tag="ACOIAS_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2700075.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTVNKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(16997..18442)
                     /locus_tag="ACOIAS_000345"
     CDS             complement(16997..18442)
                     /locus_tag="ACOIAS_000345"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGZ2700076.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            complement(18464..18817)
                     /locus_tag="ACOIAS_000346"
     CDS             complement(18464..18817)
                     /locus_tag="ACOIAS_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2700077.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(18846..20078)
                     /locus_tag="ACOIAS_000347"
     CDS             complement(18846..20078)
                     /locus_tag="ACOIAS_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2700078.1"
                     /translation="MNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMVFVGSL
                     VTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVAGLAGA
                     GLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGEDSALP
                     DFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFVLLNFL
                     VVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWITLGGL
                     GATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPMWIGTV
                     VISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFTVNQDVRGLIVLAINIVLLFMIWY
                     PFFKAYEKEEIKLESQEV"
     gene            complement(20101..20418)
                     /locus_tag="ACOIAS_000348"
     CDS             complement(20101..20418)
                     /locus_tag="ACOIAS_000348"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2700079.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            20660..22573
                     /locus_tag="ACOIAS_000349"
     CDS             20660..22573
                     /locus_tag="ACOIAS_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2700080.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     DLFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(22696..23850)
                     /locus_tag="ACOIAS_000350"
     CDS             complement(22696..23850)
                     /locus_tag="ACOIAS_000350"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGZ2700081.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKDTIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(24110..26725)
                     /gene="mngB"
                     /locus_tag="ACOIAS_000351"
     CDS             complement(24110..26725)
                     /gene="mngB"
                     /locus_tag="ACOIAS_000351"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGZ2700082.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(26869..28794)
                     /gene="mngA"
                     /locus_tag="ACOIAS_000352"
     CDS             complement(26869..28794)
                     /gene="mngA"
                     /locus_tag="ACOIAS_000352"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HGZ2700083.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AVLLIITRKQKNQRKIH"
     gene            complement(28924..29649)
                     /locus_tag="ACOIAS_000353"
     CDS             complement(28924..29649)
                     /locus_tag="ACOIAS_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2700084.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEVAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(29912..30682)
                     /locus_tag="ACOIAS_000354"
     CDS             complement(29912..30682)
                     /locus_tag="ACOIAS_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HGZ2700085.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(30684..32132)
                     /locus_tag="ACOIAS_000355"
     CDS             complement(30684..32132)
                     /locus_tag="ACOIAS_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2700086.1"
                     /translation="MKRFLNKMMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVP
                     AISNISNIVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNA
                     GVFGGIIVGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIY
                     PQAAMNQFGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVI
                     RGEIPRFMAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALT
                     SFMATVTEPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSF
                     SYGDKAWLVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLS
                     HGNFSYMAKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLS
                     NTSVQIIIGTDVVKVKDEMEMQLDELRKQADA"
     gene            32502..33224
                     /locus_tag="ACOIAS_000356"
     CDS             32502..33224
                     /locus_tag="ACOIAS_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2700087.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            33421..35088
                     /locus_tag="ACOIAS_000357"
     CDS             33421..35088
                     /locus_tag="ACOIAS_000357"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGZ2700088.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYYLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(35184..36503)
                     /locus_tag="ACOIAS_000358"
     CDS             complement(35184..36503)
                     /locus_tag="ACOIAS_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2700089.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRQTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(36544..37290)
                     /locus_tag="ACOIAS_000359"
     CDS             complement(36544..37290)
                     /locus_tag="ACOIAS_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGZ2700090.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(37534..39543)
                     /locus_tag="ACOIAS_000360"
     CDS             complement(37534..39543)
                     /locus_tag="ACOIAS_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2700091.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(39937..41370)
                     /locus_tag="ACOIAS_000361"
     CDS             complement(39937..41370)
                     /locus_tag="ACOIAS_000361"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGZ2700092.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(41457..43373)
                     /locus_tag="ACOIAS_000362"
     CDS             complement(41457..43373)
                     /locus_tag="ACOIAS_000362"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2700093.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGVFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(43447..44307)
                     /gene="licT"
                     /locus_tag="ACOIAS_000363"
     CDS             complement(43447..44307)
                     /gene="licT"
                     /locus_tag="ACOIAS_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGZ2700094.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(44658..45845)
                     /locus_tag="ACOIAS_000364"
     CDS             complement(44658..45845)
                     /locus_tag="ACOIAS_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2700095.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(45859..47250)
                     /locus_tag="ACOIAS_000365"
     CDS             complement(45859..47250)
                     /locus_tag="ACOIAS_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGZ2700096.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     regulatory      complement(47394..47573)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(47845..49497)
                     /locus_tag="ACOIAS_000366"
     CDS             complement(47845..49497)
                     /locus_tag="ACOIAS_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2700097.1"
                     /translation="MKDKLQVLSGAMMIPVILLVVAGVFIGLGSAFANMENVSALGLS
                     GLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKKEKGWGALGGIVLFIGM
                     HTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGSFLGIFSYDCSIFGAII
                     AGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLGTAMYFIWPPVGLALSK
                     LGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVGGTMEVGGAIYEGVNNI
                     RLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLADNANKKKAISILIPAIV
                     TAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFNVSIMGESFRNMFPNLL
                     QPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSEEVKLYSKKEYRERQSV
                     DAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNCATRLRVELNSIDGFYE
                     DGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD"
     gene            complement(49517..50920)
                     /locus_tag="ACOIAS_000367"
     CDS             complement(49517..50920)
                     /locus_tag="ACOIAS_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2700098.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(51184..51675)
                     /locus_tag="ACOIAS_000368"
     CDS             complement(51184..51675)
                     /locus_tag="ACOIAS_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2700099.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(51701..52513)
                     /locus_tag="ACOIAS_000369"
     CDS             complement(51701..52513)
                     /locus_tag="ACOIAS_000369"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGZ2700100.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLSKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(52526..53677)
                     /locus_tag="ACOIAS_000370"
     CDS             complement(52526..53677)
                     /locus_tag="ACOIAS_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGZ2700101.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            53947..54588
                     /locus_tag="ACOIAS_000371"
     CDS             53947..54588
                     /locus_tag="ACOIAS_000371"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HGZ2700102.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            54614..56158
                     /locus_tag="ACOIAS_000372"
     CDS             54614..56158
                     /locus_tag="ACOIAS_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2700103.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(56358..56984)
                     /locus_tag="ACOIAS_000373"
     CDS             complement(56358..56984)
                     /locus_tag="ACOIAS_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2700104.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(57119..57835)
                     /locus_tag="ACOIAS_000374"
     CDS             complement(57119..57835)
                     /locus_tag="ACOIAS_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGZ2700105.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDKAATKFGLE"
     gene            58261..59475
                     /locus_tag="ACOIAS_000375"
     CDS             58261..59475
                     /locus_tag="ACOIAS_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGZ2700106.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            60095..61423
                     /locus_tag="ACOIAS_000376"
     CDS             60095..61423
                     /locus_tag="ACOIAS_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700107.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(61551..62741)
                     /locus_tag="ACOIAS_000377"
     CDS             complement(61551..62741)
                     /locus_tag="ACOIAS_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2700108.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSKNGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQSVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(62847..64226)
                     /locus_tag="ACOIAS_000378"
     CDS             complement(62847..64226)
                     /locus_tag="ACOIAS_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2700109.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(64257..65465)
                     /locus_tag="ACOIAS_000379"
     CDS             complement(64257..65465)
                     /locus_tag="ACOIAS_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700110.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT        21589 a        11726 c         7226 g        25560 t
ORIGIN      
        1 ataattataa ataatttatt aaattattta agaaaatata caattgtata ccaagtcaaa
       61 aacgacaaca aataaataaa actaaaaaag atgttgtctc aaaatataga taaatttatt
      121 tttcattgct aaaatgatta aaatatattt gatttaatat acttgattta atataaaaaa
      181 agagtgtctc ataaactaaa aagttcactt ttagacactc ctttatatat attatctact
      241 tcttaattca tttattacct ctctacattt tctttcaact agtggataca tctttataag
      301 tactaattac tccacttaaa aatgattcag gtactgtcca tcagtcatat ttttctgcca
      361 aatataatgg tgtttcataa tgagacaata aatatttata tctctactta tcttctctaa
      421 gctcgtcaaa tccaactata ggtatattta aattatattc tttaagtagc ttttctagct
      481 cttctataaa aatacctttc ccatccacca tttgtatatc atttatttta tccatcaatt
      541 cattgcaacc acttaactca ccaaaccaac gtttagaact acatgtctta ttataaccac
      601 aacttggact gccatctatt gcaatgatac tttttatatc ataatcattt tttaaatatt
      661 catcaaactg ccttacatga ggtattaaca tttgcctaca ttgttctcta aaaaacaaat
      721 tgtcaaactg ttcttttaca tgtccccacc tatttattcc atacataatc atttctgggc
      781 atggtagttg aattattcca taccctttat ttattaaatc tatcgctata ttttgtgctc
      841 ccttaaattc tgacaatccc tctactttag aattacaatt taatatacaa tgacatacta
      901 ctacaatctc tctatttctc ttcataataa tctccttagt tattcttaat tttaatgata
      961 ctacatattt tgataattat attatttatt tgttctatat ttccaatatt ctatatagaa
     1021 atatacaatt agtaatcttt tataatctat actatgataa atgtatatat ttttttagga
     1081 ggtattataa ttggatacta aaaaagtcac attttcttca atgtgcatag ttattaatat
     1141 tgtcttaggt atggttatat ctatgataaa tataccatta ttatttttag atacaattgg
     1201 tactgtactt ggggctgtcg tattaggtcc tttttggagt gctcttattg gtgggtgtac
     1261 aaacttagtt ttaggtgtta tatcaggtcc tacaaatata ccttttgccc ttgtaaatat
     1321 tgttcttggt cttattgttg gatatatttc taagaaaaaa ggttttggtt ataaagaagc
     1381 tattattaca ggagtaattt tatctattgt ttgtccttta attggtactc ctatttcagt
     1441 tttattgttt ggtggtttaa gtggaagtgg tgctgattta ttggttggtt ttttagttca
     1501 gtctggtcag aagatattta ctgctgcgtt ctttccaagg attcttagta atatagtaga
     1561 caaacctttg tcttgtatta tggtggtata ttttataagt aaaatgccta aaggttttat
     1621 aaaacaatat agtaaaaata cttctagttc atattaataa atcatttatt atgtaagatt
     1681 tattactctt taaaatgcta tatactcatt tttatataac atttttatta ataatttaat
     1741 tagatagtat ttgtattatt ttaattaaac gtaacctctt gatataaatt tgagctataa
     1801 atctatatca agaggtgcta ttttattttt aatataatta atattctatt acgcaaaaaa
     1861 tacatatatt tcattatttt atggaaaata ttacttcatt taactctata tcaaactcat
     1921 taagtaaaaa tctaattata tttaatttag cctctgtatt taaatttgtt tctaaatata
     1981 tattttctga tattttaata ccttttctta atttatatgc atctctagac ttaatattat
     2041 ctataaaatc tcttttataa gtcttgtcta caatactgtt aaaccttaca gaatccaaat
     2101 catataaagt aagagcaact tgttcataaa attctgtcca atttttagcc ttatatgact
     2161 ctcctaatat actaaatgat actatacttt catttgtaaa attaacatca tcatcctcta
     2221 acgtaaatat attttcactg ttacttggag gttcatatgt aacacttgga tatttccaaa
     2281 tttctacagc tctatctagt aatatcttag ttctattttt tatttcttct tcattccatt
     2341 tatctataga tgaaatatat ttatttagat aaagcctact atctttaaat ccatcttcca
     2401 tttctttctt ttcttcaaat gttttattac ttagttttga attatatcct gtcaaagtta
     2461 aatttccaat tgtatgtaaa tatttatcat gtatctcctt atagttatct cctagtgatt
     2521 ccttccattt atttgttaat gtctgtggca ttatatgctc tatatttagc atattattat
     2581 tgattaaatt ttctaaatca acaatttctt tattattata attctctaac ctctcaagta
     2641 tatataactt attctttgac ttagtattat atatatcttt agtttcaaaa gcatttgaaa
     2701 attcactgtc agtaggaaat ctttgagatg atttcttatt tataaaaata tatcttaata
     2761 tattagaata gttttcttta taatcactaa attttttaat ttcccttcct aagttcataa
     2821 atactttatt cagtgcatta ggaggtacat cacatactat tcttcttaat ataaaacatt
     2881 ctactaaatt aagggattct acaagtactt ttttatttat tataccttca ttataatcat
     2941 caaataattc taatataaac ggatatgata caaatatttc aagtttgttt atccttttca
     3001 aaactttgtt tacatcatta tcatcagtac atgaataaga tatctctcta taatagttag
     3061 aaaatttaag taaatctttt aataaaattt ctatatctat accattatct gatacatacc
     3121 ttttaaaact aaaatatact ttatttttat tcggtatact tctctccttt atagtcaaat
     3181 agtctcttat aaaactactt acgttgctac ttgtattttt ttcaatttta taccaataat
     3241 tattataaaa ttcctcttgt aacttagagc ttctacccat taacacaaag tttcttactc
     3301 tatcagcttc acttaaatct aagccagttg aatttagact ttcaaagatt aattgagggt
     3361 catcttcacc attttttaac tctatttcta ctatagttaa tttctgtata gcatcaaaca
     3421 aatcatctat tgatatatta tcttctttta ttctattata aaaatataaa taatttgaaa
     3481 ctatattaga atttctatct gtattcattt cttcttcaaa tagttcatag tacgcttttc
     3541 tatcttctgc tattggtttt aactttattt ttccatcttt atctcttgag tgcttgttta
     3601 taagataaca ctccttaatc tgttcttctg ttgtaataat tccttttgat tccccaaact
     3661 cttttattat ttgacacatg gcaatcaata atattgacac agtagtcact ctttgttgtc
     3721 catctattat taaatattct tttcccatac tatatataga tacaactgac cctaagaaat
     3781 gagttctaaa tccagaatct attatatcct ctaaatcatc aaacaatctt ttacattgtt
     3841 ctttcttcca gttataattt ctttgatata caggtactac aaaacttttg tttgacccct
     3901 ctaaataact aagaaattca atttctcctg ctttcatatt aactccccct ataagttaaa
     3961 taaattccat tcataactcc atgtatctat atattatatc aaaattttga ctcataaatg
     4021 tatatgaaag ccttattata taccttttta tacctgtttt tattttatta aagtaaatac
     4081 atgattattc tataaaatat acaaatactt aattttaaaa attattattt aatcttcttc
     4141 atgagtaatt catagttatt tatcaattaa aaagagatat tgcataatta ttcaatacct
     4201 ctctttgtat atttacatta tccatatcta gttatctatt tcaacccaat attagactac
     4261 ctactaataa atttattctc tacatcatta acaataattt tgcactaaat aacatcatta
     4321 gcctatctct gatgcatttt ctttaatttt atttatagtt ttaatcaatt tatttctctc
     4381 ttcaatatga ttttcaaata aatcaattat acttccaaca gttctaaatg ggtcttccat
     4441 caacactttg ttatcctcta taaatccatt cttgactaca taatcgacta taagtttaac
     4501 aaaatgtatt tgcttagtat taaagctctt attatttaaa aactctgaaa aagcttcatt
     4561 tgcagcttct ctatccaatc ccactagtct tctcactaat tggcttactg gagtatctcc
     4621 aaactccttc ttataatcat cacaattacc taactgtttt aagagtattt cctccaacgt
     4681 ttcaacatct tgctttgtta tctttttatt atttcttaat ttaaatattg ctaattcatc
     4741 tttatggcta tttaaataat actctacctt ctttttataa ttcttaagat tgtttgcatt
     4801 atatacagaa tcatttctat cttctgctac aatcatatct tcaaaacttg tataatatat
     4861 cttttgacta gacttttcaa gatacttaat aagctctctt aatgatatac gtacttcttc
     4921 tacctcaaat atatctgaat cttcccaaaa atcctctgtc attgcttttt caatagtata
     4981 tttttgttgc tgaatctgtg gtatagtccc aagttttgaa agtttttctg ctgtagccat
     5041 tacatgcctt atacctttac ttgcattatt attttgtaag tatgcaagtt gtatattata
     5101 cataactaca tcaaatcttt tagccaattc atcatcacta tatgaagtta tgagtggtga
     5161 tatatattct cttatctcat tttgtgctac tgctccaata ttgctccaaa cattttcatt
     5221 tttatactta tgtacataac ttaaattcat cctaactata taactatcct catttaaagc
     5281 ttttacagaa gttataagtt cttctaacaa ttcttttcta agttctgcat actcaccttc
     5341 tatatattta atatcttgaa gttctttaac catacttacc tttatattaa ataatttttc
     5401 agtaagtgtt tctgttttat ttccctcaaa tcctttagga ttaaatttaa aaaattcaaa
     5461 attattacaa aaatcaaata ttaagaatct ttccttatcc atatcaacac ctaataaatc
     5521 tggacataat ctagttcctc tacctatcat ttgccaaaac ttagtcttac ttctcacttt
     5581 cttgaaaaaa acaagattta gtatttcagg tatgtcaatt cctgtatcaa gcatatctac
     5641 tgaaactgct atttgaggta atttattctt atcagaaaaa tcatctatta tagaatctac
     5701 ataatttata ctatagtcaa ctgcttttac aaagttccca ccatactttg gatacagttt
     5761 attaaatctt tcaactattg ccctagcatg ctttgtattc tttgcaaaaa ttatagtctt
     5821 accaatcttt tcttctcctt ctattttcaa cccctctgtc attagcttat ttaaaactaa
     5881 atctattgtg tcagaattaa atagccactc atttactgca ttattcccta tttcgtctcc
     5941 aatactctca tcatcattaa atgtattttc atattcttcc ttctcttcat cagaaagctc
     6001 atcatactta attccatctt ccataatctt agtcttaaac tcaatactag tataatctac
     6061 taaatatcca tcctcgactg ccttattata ctcataagca tatgtaggta ctccattttc
     6121 catgtcaaaa actgaataag tatttttatc tatctcatcc tttggtgtag cagtaagacc
     6181 cattaagtaa gaatcaaaat aatcaaagat tgacttatac tttttataaa tacttctatg
     6241 cgattcatct attataatta aatcaaaatg accaggtgta aacaatctct ttccatcctt
     6301 agacttcgta tcatcaattg cattcatcat tgttggatat gtagaaaata tcatcctagc
     6361 atcttctggg ctgtctttgc tatccaataa attacataac gccaagttag gtaatagctt
     6421 agtaaaactt tttttagctt gctttaccaa tgcttttcta tctgctagaa ataatatatt
     6481 tttaatccaa ttatgtcttg aaagtacatc aactatagat atagcaattc ttgtttttcc
     6541 tgtaccagta gcacatacca aaagagtttt gcgttgctta ttttcaagag cctcacatac
     6601 agccctaata gcttcttttt gataatatct attggatatt tcatcattta ttgttacaga
     6661 acttaaagac ttcttactct ttcttctatc tatcataagt tgtaattctg attttttata
     6721 aaatccataa actttcctat ctgaatagtc atcccaaata tacatatcaa aaccattagt
     6781 caaaaatatt accggtcttt gactatatct cttttccaaa caatctgcat ataatttagc
     6841 ttgttgttga ccaattttcg ggtctttact cgttcttttc gcctcaacta ctgctaatgg
     6901 cttaccattt tcaccataaa gaacataatc tacatatccc acaccacttt cattaggcat
     6961 accttgtact tctatttctt cccatatatc ttttccaaat tcccaacctg caagctttaa
     7021 ttctatattt atatatcttt ttcttgtctc aaattcgctt acttcgtcta tcttgaaatc
     7081 atagtttaca gtattaactt ttctcttttc agttagtgac tttcttaatt cctcattttc
     7141 ttttattact tcttcaagct ttttatcttt tgaacttaat ctctcataaa gatttttaag
     7201 ttcgtctggt ctcgtccttt tctcttcccc atgaagtaat aaatcttcat taaagtcttc
     7261 tgctgtatat tcatctgaat aacaatagtc tatccaacta acaaattgat gcaaattgtt
     7321 aagtgaaagt attgcttctc ctctctctat gttcgcacta gtatgtacag ataaattacc
     7381 taatttaact atatatttta ataaaggtaa catatcatag tctataagtt ctataaaact
     7441 attattatga atcaacgtac taagattatc ttgataaggc aatactaaat cactatcatt
     7501 agcatatagc cattttactg caagctctaa agctcttctt gttatagttg cacaagtaga
     7561 tgggctaact agtatacttt tttctgcttc taagcaagca gtattaaaac tattgaaatt
     7621 tttcttcttt tgtaaaaaat caaaattacc caaggctttt atcccccttt tgtcccttag
     7681 ttaaaataaa tctctctaaa aactatttct tacagctgtt tcttatacta acaaatactt
     7741 cttcattttc tgtaaacaat tcgtacatat cttcaaactg tggtatatca tatccatgag
     7801 aatcatcatt tctcaatttt ctaagtgttt ctataactat agaagaattt ttaggtaata
     7861 aattgtgata ctcacaagct aaaaggtagt cataaaagtt tttagatttt atgtttaaat
     7921 caattgattt ataatacaac attgcttttg ttaattctag aaatgatacc atatagcttt
     7981 taaagccttc ttttatgtat ctagcccata actcaggcat ttcttcctta tcatataaga
     8041 ttttgaagtt ttgcaggtca tacaagcaag aaccaaaatc cctatttact tcttctattg
     8101 agttccaaga taatttgttt ctaattacca tacatgtccc tccaatctaa gatatctatt
     8161 acatcactgt tttcaaaata gaatttattt atataatcct taaaatcttt agatttataa
     8221 ataacttcac cttctaaaat agctttttta agtgctattg tataattatc tgtaacaatt
     8281 tttaggtcta catcaatgtc taatatattt cttagagctt ctgtcttagt tgcaagttct
     8341 gtaattggta cttttttgat gggaatccaa gctatgtcta tatcactttc tgatgtatgt
     8401 ttatctgttc cataactacc aaatattatg attgctagag tattttcttt tggaaataca
     8461 tttattattg agtttttaat ctcatttggt atatttctca tttaatcacc tcttgtttat
     8521 taggtaatat tgcttaattg aatttatatc cataatcttt taatctattt atacatattt
     8581 ctctttcttc atcagtaaac aactctttat acttttcttt taatactaat acttctaatg
     8641 ataaatctaa tctcttacac tcccatagcc ttgtaaatcc ttcagtagct ccttctttat
     8701 tgattaattc tttagcagct aataaggctc ctttgttatt tatcagttgt aaaaatcttg
     8761 ttggcttata gttacattgt aaatcagctt ctttatagat atttaccatg ttatggtata
     8821 aatctagctc tatttgattc atagatttta ttcccccatt ttaataattg tattttagtt
     8881 gaataactat cctttaaaag ctttttgcat taatgagtta aagttgtctt ctagttcttt
     8941 tagacttgtt tttattccta ctttcaattt gtctacttga ttaacaaact cttcaaactt
     9001 attttgaagt tctattgatg gtactggtat taatattttt tgaagttctt tgcttctaat
     9061 tccttgtgca gagctaccag ttttatattt atctatttct accctaaaag cttttgtttg
     9121 catatattgt gctaaaaaag cattatttaa aattttttta tctccctgaa ttgttataat
     9181 cctttgaccc atagcaaact tttttaaatc ctttggtatc ctacatacat ttccaaatgt
     9241 atgaccttct gtaacaaaaa gtacatctcc agctactgga aaacctcttt tcatccaaga
     9301 attataggtg ttttcagata tatattcatt aatatattta aatacaccat tccctacaga
     9361 ttttgcattt attattctaa ttccattctc tgaaagaatt ggagttttac cacgataatc
     9421 aataaaattt gtacaatctt tcaataatac aaacttttgt tccttaaaat ttccaaacat
     9481 ttcaataaat cttgatttaa ctaattcatc caatgcttct atttgctctt ttcttttatc
     9541 tattaactct tgagctttat ctaaaacttt tgatatatct ttttgagctt ccatttcagg
     9601 taaatctact tttaaatttt ttaaatcatt aactgaaaaa cttgcttgat taactgattt
     9661 ttttattatc cttggtaact gtcttctaaa ttcattacta ttaaaaaaat aatttaaata
     9721 ctttggataa caaacttctt tatctggtcg taaacataaa agattcattc catgtataat
     9781 ttttttattt atatcttcat atattgcact tttccctaaa tgtttttcac tattaatatg
     9841 tgacattagt atatctccat ttttcaataa atagtcttct aattttccta actcttttat
     9901 gtcagcatat cccattttat tagtatcaat ataagagttt gctatagttt ctattcttgt
     9961 aattggatat ccaaattttg tatcaaattg tttaatacta gccccatttc taataaattc
    10021 aaaaacttca ttcaatctat attccatact ctacacctca agcattcttt ctaattcctc
    10081 taatccatct acgatttcct tctctaactc cctaactcta cctataataa cactaggagc
    10141 atcataaact acttcctcat acactacctc tttatactta ttaatagata aatcataccc
    10201 attctctctt atctcttcca catcaacaaa aaagctttgc tctgttcttt ttctatctac
    10261 ttcaccatca agattatcaa atctcttaat aatatcctct atatcactat cctttatctc
    10321 atttctctta tcatccaaag aataaccatc agctttcata tcatagaacc ataccttatc
    10381 agttccacct gttcctgttt tagtaaatat cattatagct gtagatactc ctgcataagg
    10441 cttaaacact ccacttggca ttgatattat agcttccaat ttatgattat caactatttc
    10501 ttttcttatc gatttatgtc cacctgttga accaaacaat actccatctg gcactataca
    10561 tgcgcatctt ccaccaattt ttaaaactct taaaaaaagt gccaagaata ataactctgt
    10621 tttctttgtc ttagtcactt ttaataaatc tgctgacact gcttcataat ctaaacttcc
    10681 cttaaaagga ggatttgcta acaccaatgt aaacttatct ttatctgtat taacctccga
    10741 taaagaatcc ttatactcta tattaggatt gtcaacgcca tgcaacatca tattcatagc
    10801 acctattcta agcatagttc tatccatatc aaatccatag aacataccat tattaaaatg
    10861 ctccttcagc ccttgcacta agaaaagact tgaatgatta tcccttaagt attgttgtga
    10921 tgatactaaa aaacctgccg aacctgctgc cgggtctact ataatatctt ctggtgttgg
    10981 tttaataagt cttactatca tatctattat gtgtcttgga gtcctaaatt gaccattagt
    11041 acctgctgta gccactttgg ataataaata ctcatacaaa tctccctttg tatctctatc
    11101 cttacttaat tctaaattac taataccatc aactatttta gataacataa gaggcgtagg
    11161 aattttaaat attgcatctc ccatgtactt tgcataagct gattctttat ttccatgtaa
    11221 tttttttatg aaagggaaca cttcattttg tactatttca tacatttttt ctgaatttcc
    11281 ttcattagaa aacttactcc accttaaatg ttgatgttca tcatcaaata tcttttcaaa
    11341 agaaacaccc aaaagagttg cttctgcttc attttttgtt tctacttcat ccaatccttt
    11401 aataaataaa agatatgtaa attgttctat aacttctaat ggattagtta atccaccagt
    11461 ccaaaaagtc tcccaaatct tgtccacttt actccttaat tctcctgtaa tcatatatga
    11521 ctttccctcc agtgttcata aaataattta aatatatatt ttagtcgtac gccttaatag
    11581 ttagtgtctt atgttaaatc caagttttta ctacacaatt attaaattat ttttcctatt
    11641 tacatatttt agctaaaaaa ctaatgcaat actcttattt tatgattatt atatatacca
    11701 tacaaaatta ttttatcata tatccatcat agaagtcatc tacctggata actcttacca
    11761 tattataact ttttaatatt aatttaaact tagaaagaaa ttttttattt aattatatca
    11821 tcttccttat ttcaaatttc tcaattacaa tcttaatcaa aaaataaaat aagatgctgt
    11881 ctcaaaatag atataatctt cttttttatt gtcggaatga tgaaaatata tttaatttca
    11941 tacaaaaaag agtgcctcat gaactaaaag ttcattttga gacacccctt aatttaatct
    12001 tacttattct ttccctttat tattaacact tcactatctc tattgctttt cctacacatc
    12061 cacaaacttc catcctactc ttaacaaaat ctttaactga ctctaaacct tttttaccat
    12121 attttttagg gtcaattacc tctgggtcag aattaagcac atccttaacc ccattgctat
    12181 aagcaatcct taattcagtt gcataattaa ccttgcaaat ccctcttttt atagactcct
    12241 taataacctc atctggtatt ccagacccac catgtaatac taatggaact gatacaacct
    12301 ctctaatttc agataatcta tctaaatcaa gttttggctc tcccttatat agcccatgtg
    12361 ccgtacctat tgctactgct aaagatgata cccctgttct ctctacaaac tcttttgctt
    12421 ctaatgggtc tgtatatgct ccaccatcac caccatctaa atcgtcttcc ttacctccaa
    12481 ctttacctaa ttcagcttca acaggtatat ctgaaggcat acatgcatct acaacagatt
    12541 tagatattga aatattatct tcataacttt catgagaacc atcaatcata atagaagtat
    12601 atccaactct aagtgcctgc atagctagtc caaagcttga accatggtct aagtgcattg
    12661 ctactggaac acttgccttc tctgatgcta cctttacatt cgctaaataa taatctagtc
    12721 ctgcatactt tattgttgaa ggtgttgttt gcaatattac tggtgattta agttcttcag
    12781 cagcctctat tactgccata accatctcca tattttcaac attaaatgca cctactgcat
    12841 aatgcccttc ttgtgcatct aataatattt gtttagttgt cactaatgcc ataatttctc
    12901 ctcctatatg tttatactga tttaatttct tcgttattta attttgatac tatttctcta
    12961 cttgttttgc tatttaataa ctcatttaaa aattcttcat tcattaattt tgaagatagt
    13021 tgagataatg tttttaagtg taaattatca ccattatctg tagctgctat cataaatata
    13081 atatgtgcca agcttccatc ttctgattca aagtcaaacc cttcttttga tagacctact
    13141 gctacacgtg gtacctttac tgcatctgtt tttgcatgtg gtatcgctat ccccatacca
    13201 aaacctgtag atgatatttc ttctctttta aggatttctt ctttaaactt ctttttatct
    13261 aaaagaacac tagatttatc aagtattcca atcatttcat caattacatc tgttttattt
    13321 tttcccttaa catctaaaat tactgtttct tcgctatata aagagatatt attagatacg
    13381 ttttccgatt ctgcactttc atttttatgt tcatcttttg ttacatttgt attttctata
    13441 gtatttttct catttactac tttaggtgta accatcatat aaagtacagc acctactaat
    13501 gaacctacaa atatacaccc tacccataaa agtggcttat tgactaatcc tagaaccaaa
    13561 aaccctccat gtggagctgg tacttctatt ttcattatat aagttaaaat tgatgatatt
    13621 gatgatgcaa tcataagtac tggcaacact tttaatggat tttttgctgc aaaaggtatt
    13681 gccccctctg ttatatgagt actacctagt atataattga ttaatcctgc tgctctatct
    13741 tcttctgtaa atctattttt aaataaagta gatgctattg ctattaccaa tggtggtgtt
    13801 atacaagctg cagatacacc tgccataaat aaataatttc cctgtcctag aagcatagtt
    13861 ccagttacat atgctgcttt atttactgga cctcccatat caaatgctga catacatcct
    13921 attactattc ccaaaattat agggcttgaa ttttgtaacc ccgatagaaa gttcatcata
    13981 gcattgttaa gtgctgaaca tggcccacca agtaacaaca ttgctacacc cacaagacct
    14041 acgctgacaa taggaattat aaaaatcgat ttaagacctt ctaattgtcg tggtaatttt
    14101 cttaacaaat gtacaaatag tttaactaag taacctgcta aaaatcctga tatgattcca
    14161 cctaaaaacc ccgaacctgt atcaaatgat atcattcctc caacaagtcc tgccaacata
    14221 ccagctcttc ccgatataga ttgagctata aatgctgcca atacaggaac catcaagttc
    14281 atagaatatc caccaacaga ttttaatgta gctgctattt gattatattg ttctgaattt
    14341 gggtcaaatg aataaatccc ccataaaaat gatatagcag taagtactcc acctgctact
    14401 actaaaggta acatgtgaga tacaccattc attaagtctt tatacaactt ttgaccaaac
    14461 gataaatttc cttcttctgc aattttactg cttgataagt ttccttttct aatagctact
    14521 tttccatcaa tacatctttg tataagttca ttagctttat gtataccttc tttgacaggt
    14581 acttctatta ctggctttcc attgaatctg tccatatcaa catttttatc acaagcaact
    14641 attattgcat ctgccatttc gatatctttt gctgttaact tattttctac acctatagct
    14701 ccattcgttt caacttttat gtcacaccct aattccctgg ctgaattttt taatgcttct
    14761 tctgccataa aagtatgagc tattccagtt ggacaacctg ttacccctag aatcattttc
    14821 tttcccattt ttataccacc tttctgactc ttatattttt tatataatct tccacattct
    14881 ccaaattacc tataccatta ctttcagcca cacttgctcc tgttgctgct gaccttttta
    14941 atgcttcttc aatatgttct ggattttcca accatatact taaaaaagca gctaatgcag
    15001 aatcaccagc acatgcagag ctgactactg taactggttg agcactagca taataaattg
    15061 attttccatt ataaaaataa gaaccttttt ctcctaaagt tagcaatata ttttgagcac
    15121 cttttaaatg tagtaattta agtacatcct taatatcttc ttcatctctt acaataattc
    15181 caaatatatc tttgatttct tcatcatttg gttttatcaa atatggatta tactgtagta
    15241 actcctttaa ttttggtgaa cttatatcga gtattgtctt tatactttta ttttcacaaa
    15301 ttttaaatat atcctcatag taattatcat caataccagg aggtagactt ccacttattg
    15361 ataagtaact tatatcttct aatgactgta tcttatctag catatctagt ttttgttttt
    15421 cgtttacata tgacccagag tttacaaatt taaattcatc acttccatca tttaaaaata
    15481 tatttatacg agttgtatct tctacccaag taggcaatac gtcaaatcct ctcttttcgc
    15541 tttcttcaac tatatatttt cctgaaaatc ctccaaaaaa tcctaatatc ttagattcta
    15601 taccataatg acctagtaca aaagttacat ttaatccctt tccattagga gaatatacag
    15661 catttgatgt acgatttact aattttctct ttatcccatt agtagatata ttcatatcta
    15721 ttgcaggatt tgtagttaat gtatatatca ttgtcttccc ccttgcttgt gctgtgtatg
    15781 ttttcattat aataaaaaag tctatgaaaa tcttttcaca aatagaaaat cgattttcat
    15841 agacttttca atttaatttt cagaattatt atatctgacc atataatcta taattatcct
    15901 agatataata tacaagggca atctggaaga tacttcatat tcagaaattg atatatgttc
    15961 atgtttgtat cctacaatat ataaatccga atactttata agttttgcac tttcactaca
    16021 acagcaagtt ataaccttag ctccaactaa attggcattc tgtgctgatt ctacaagctc
    16081 tattgtattt ccattcaatg aaaatataat tataagttcg tcttttttta ttgatttaga
    16141 ttttattttc attatgttgg ggtcatttat aaaaacaaca ttaaaatcaa gtaattgcaa
    16201 tttaagacta aactcttttg ctacatattc agtaagccct cttgctaaaa tatatatctt
    16261 actagattta tttacagttt ttattatatt gattagtgta gttaaagata tttgttctat
    16321 aactctctgt gcttctataa aggatttatt tattatatcc cccatatttt gtatatcatc
    16381 attttcatct tttactgtta atctatacct aagttcattg aatccattta aaccacattt
    16441 tctaattgct cttgaaacgg ttgctggaga tgaatatgtt tcatgagcaa tctctactat
    16501 agataattct ggtaatattg attcattttc atttataaat ttaattattt caagttccgt
    16561 cttggttaga ttttcagaat tacttttgtt tagctcaatt atcatatgtt atctcccctt
    16621 atataagttt tatttgtcta aatttatcga gtaatataat atattacctc ttaactactt
    16681 atttttatat acaattaaga tttagtcaat gtctatatat gtaaatttat aatataataa
    16741 ttttttgctt aaagtttatt atgttatctt atagcatttt aaaataaaaa agaattgtac
    16801 ctctaccaaa atttggatga agtgcaatag atattcctta tactgatatg tttatgatta
    16861 aaagtataaa tataaatttc gctatgaatc atataattaa taaatcattt tatttacaat
    16921 tataaccccc cttataaaac tatattaata atatatacag tatttataag gggggttgtt
    16981 tatatttaaa aactatttac ttttctttta attcttctcc attagttctt attacatttt
    17041 gataccaata aaaactatct tttttataac gttttttact tccatttcca ttgttatcta
    17101 tatctacata tataaatcca tatctttttt ccatttcttg agatgaacaa cttactatat
    17161 ctattggtga ccacatacaa taagctaata tttcggttcc atcagctata gcttcaccta
    17221 tttgttctat atgttttcct aaataatcta ttctgtaact atcatgaact tttccatctt
    17281 caaatttatc atgcattcca aatccatttt cagctatcat tattggcttt tggtatctat
    17341 cataatattg agataaagta ttgtataatc cttttgggtc aattgcccac ccccaaggat
    17401 ttgcttttaa atatggattc tttatattgt cagttggatt catggtgttt ttacttgctg
    17461 atactgttgt agaataatag tatgataaag ctagaaaatc catagtattt tcttttaaga
    17521 ttttatcatc accttcctcc ataactatat ttatgttatt ttctttgaaa aatctcttaa
    17581 tatagactgg atactctccc ctaaaactta catcagtgaa aaaatattgt agcctatttc
    17641 tttttaaagc aaatactaca ttttcagggt cacaatcttc tggataagat gtacaatctg
    17701 ccatcatcat acctatcatc atatctgaat ttatttcttt tccaagcttc ttagtcaaag
    17761 ctgtagctat gaattgatga tgaatagctt gatatctagt ctcttctaca ttatctactt
    17821 ggtctacaca aaatgcaact gaattaaatg attcataatg tattaaattt atttgattta
    17881 taactatcca atatttaact ttatttttat atctatttag tattaattct gcatatctaa
    17941 caaagaaatc taccactttt ctattagacc atcctttata ttttaatact aaattcaaag
    18001 gagtctcata gtgaagtagt gttacaatag gttccatacc attatctatt acttcattta
    18061 taagattatc ataaaacttt aatccctctt cattaggctc taaatcatct ccatttggaa
    18121 atattctagc ccaatttatt gaaaatctaa aacactttaa ccctagctct ttcaaaagct
    18181 ttaaatcttc tttgtatgta tgataaaagt ctattccact acgcttagga taatatccat
    18241 tattatcttt catagcaaac tttattcttt ctatagtcat atcagactca taaggtttag
    18301 atatatctct atctttatca tacatatgta catcagctaa acaccaacct ttgccacctt
    18361 cattccatgc accttctaat tgattagctg ctacagctcc tccccataaa aaacctttag
    18421 gaaacttacc caatttaatc actccttatt ttatttattt aaatcattta ctattattta
    18481 ccttatattc tatagacttt actattccta ctaaagacat agttatattt aactcgctga
    18541 atatggtcat aagagtatct tgagcatgtg caaataacag cgaatgtgct cttttctctc
    18601 caccagcttc cccttgtata atcttggttt gagctctatg tgcttctgtc ataaaccctt
    18661 ttgctttagc tagttttaaa tcagcatttc tataatcaaa cttttccaaa tcattaagag
    18721 catgtttaat ttctgttcta gcctcacctg cacgaagtat tatttgcatt gccacctcta
    18781 ctaattcttc atctattata tgttcttcaa ttgtcatatt tataatttcc tttcaatttc
    18841 taaatttaaa cctcttggct ctctaatttt atctcttctt tctcataagc tttaaaaaat
    18901 ggataccata tcataaatag caatactata tttattgcta aaacaataag acctctaaca
    18961 tcttgattta ccgtaaaagt agatatacca aaaggaacat accataaatt aaacacctta
    19021 cttggaattg tcacaaatcc catactcatt gataagtaag tagttattga tattactact
    19081 gttcctatcc acataggtaa cattaaaaat gggttccatg ccactggagc tccaaatatt
    19141 actggttcat ttatattcat tatagaaggg acaatacatg ctcttcctat tgcttttatt
    19201 cttccaactc tggataaaca catcattata actaatggta atgtagcacc caaccctcct
    19261 aaagttatcc aacctgtttg aataacttcg taaacatata tattagttgc tactcctcct
    19321 gatgctactg ctgctgcatt agctgcaatt gcaccaagtt gaattgaata agttatagga
    19381 gatagtaccc acccacttat tcctaaagaa tatataaata ctactaaaaa gtttaagagt
    19441 acaaatccag gtaatgtctg tgctatatct gatactggtt taaatactct aactattatt
    19501 tcaaacatgt caaagttgag tccataaatt aatccccagc ctacaaacat tatcactgtt
    19561 ataggtaaca tattatcaaa ccacttgctt acaaaatccg gaagtgctga atcttctccg
    19621 aaaaatgaaa aagaaccaaa taaggacatt attaatccag agaataatcc tgcaactagc
    19681 gatacaagca ttccttctcc tccaaacctg ttgaagttaa atatagctcc ttcattcgta
    19741 atctctggac gaatcatcat taacattaat ccagcacctg caagaccagc gactacactc
    19801 atactttcaa atttcttatt cttcattatt tccataggca ataaaaatgc tataaataat
    19861 cctaaaagtc caaatgtata ttgatttatt ggacttaaat caggtaaaaa gcttatgtaa
    19921 ttcttcaaga cagaaactat agttactaat gagcctacaa ataccattgg caaaactttc
    19981 attatagttg attgaactga ttgtacccat atattcttag tcatattatt tacaacagga
    20041 gtaaattttt cacttaaaaa atctataaat ttattcataa acactctccc cataaatatt
    20101 ttatttgttt gatattagtt ctagtgcttc ttccaaagcc tttgccccat ctagtcttcc
    20161 atatgcagtt ttagatatac atgctactgg aacgttatat tcattagcct tttgttgaat
    20221 ttcttccttc atataatcta aatgtggtcc tagtaataaa caatctattt ctgataaata
    20281 cttatctatc tctgactcac ttctcgcttt tatatctgct tctatacctt ttttctttgc
    20341 actctttctc atactagttg ccataaaacc tgtacttgct cctgaaccac acgccaaaag
    20401 cactcttata tttttcataa tgatacctcc aatattttta tccatcaatt tatttcttaa
    20461 tgaacttttt attttattat gtttatattg tacttaccaa ataagttcat ttcacataaa
    20521 tatatttcct aataatagga aatcagttac tcttaattaa acatattaat atagtataat
    20581 gtatcttata atacgtacat tcaattatat aattatcata tcatccagat tttattttaa
    20641 aactatgcga gggaatctaa tgaaaacatc taaaaaagaa ctatttttaa atttcataaa
    20701 aacacaacat gattggatag attcatctac attggctaat tatcttaatg tctcaacgcg
    20761 ttcaattaga aaatatgtaa acgaaattaa ttccaatggt gaatttatat tatcctctaa
    20821 aaaaggatat aaagtaaatc tcaataataa ttgtcaaacc aaggttgata gttcagaaaa
    20881 tatatcacct gataacaggt taaatttaat tttaaaagaa cttatagtta atagtaatgg
    20941 aattaatata tttgatttaa gtgaagactt gtttgttagt ccagctacta ttgaaggaga
    21001 tatagtcaaa gctaataaat ttataggaag ttataattta aagataaagc aatcgaaatt
    21061 tttattgaaa ctaattggaa atgagtctgc aaagcgcaaa ttaatgagct ctataatatt
    21121 taaagaaacg ggaagtgatt ttttaagctt atttgatgta caaaaaatat atcaagagta
    21181 taatttgacc aaactgaaag aaaatataat atatatatta aaaaaataca atttgtttat
    21241 aaatgagtat gctataaata acatacttct tcatttaatg ataactatag acagaataaa
    21301 aaagaataac tacatagaat ctgtcgaagt agtaaactac atagataata ataatgatat
    21361 aagcatttca agagatatat caaatttttt agagtctgaa tacaatataa ctttaactag
    21421 tgcagaatta tactacttag tatttcagtt aacaaacaaa accacagtct taaattatca
    21481 tgaaatggat actaagtcat tgccaaatta tatagatgaa cattttgtta aattaactaa
    21541 aaaaataatt aaaaatgtat atgacttata ctttatagat ttatctgatg atgaatttgt
    21601 tgtaaaattt actcttcatg taaaaaattt aatcagtagg gctaaaaata atcaagtttt
    21661 gagaaatcaa atacctcaga agttaaaaga ttcctaccct ctgatatatg atatatctgt
    21721 ttatatttgt aatcagattc aaattttaga aaacatagaa atagatgaag atgaaatatc
    21781 gtatatatct cttcatgtag gttcattttt tgatagacaa aaattactag aagataaaat
    21841 tctctgtgca ttaattactc caaattacta tgatttacaa ttcaaaatag taagagacct
    21901 tgagaagaga tttaatgaat ccatagaaat tattcagatt ttttcagata ctcataatct
    21961 tgattttgac aataaggtag acatggttat tactacttta ccaattagcg atagatgccc
    22021 tatacctttt gtttatgtaa atccttacct aaatagaaag gattatgata atattcaaag
    22081 taaatttatt cagattaaag atagaaaaaa tatacttact gtacaaaatc atttagaaat
    22141 gtactttagt gaatctttgt ttatgaaaaa tatatattta gatagtgcta aagattatat
    22201 aagatttatg ggaaatattc tgtacgaaaa taaatatgtc aaacccaatt atatagatga
    22261 tgttcttata agagaaaaaa tgtcttcaac tgcatttaat aacaatgtag caattcctca
    22321 tagcatgaaa atggatgctt taaaaaccgg agtatgttta attgttaacg ataaaccagt
    22381 taaatggggt gaggaaaaag ttcaaattat agctatgata cctataaatg aaaaagaaaa
    22441 agaaaagttc aattacatat ttgaaagctt tattgaaata ttatctgagt ggaataatgt
    22501 taaagaactt actaaggcag ataactatag tagttttatg aatagaatag cttatcttat
    22561 tcaaaatatc taaatcaatt aatttatttc caatatatgt ttcttaagtt attaacataa
    22621 aatagataag ctgtcacact aaaaagtcta aattaatatg tgacagcttt ttacctaata
    22681 taccttgttt taatactaca tattttttga ctgttgtata ctagttataa gattgaatat
    22741 ttgtgttgtt gtagctgtca tattctcact agcagttgct ttatccattg cttcttctat
    22801 tgtcatagct ctatttacta tagaaaaata agcatctata ccctcttcat tacatttaac
    22861 tgcatcttct gttgtggctc cagccaaacc tattactttt accccatatt tttttgccag
    22921 ttttgcaact cctattggag ccttgcccat agcagtttga ttgtccaatc taccttcacc
    22981 agttatgaca aaatctgcat cttttaactc ttcctctaat ttaatttctt ctaatattat
    23041 ttttattcca gattctaatt ttgaatttaa aaatgctaaa aaagcaaaac ctaatcctcc
    23101 tgctgctcct gctccttcta cattagctac atctttactt aagtaatttt taactacttt
    23161 ggaaaaattt ctaagcccat tatctaactc ctcaattatt tctgatgttg cacctttttg
    23221 agacccgtat atgtatgcag ctccattttt cccaaacagc ggattattta catcacaggc
    23281 tattttaaac ttacattcat ctaattcctt taatctattt tctattttta tatgtcttat
    23341 ttcatttaaa acttttccac ctaatcctac taacttgtta ttttcatcat aaaactcaaa
    23401 ccctaaggct tgaagcatac ctatcccagc atcattagta gcacttccac ctatcccaac
    23461 tataaaattt ctatagcctt tatttattgc ttcttttatt atttccccaa caccataagt
    23521 tgtagtgtat aaaggatttc taagttcagt cggaaccaat gttagtcctg atgcttgagc
    23581 tatttctatt attgctgtac ttgtattttt taataatcca tatgttgcat ccactttttt
    23641 agctattggt ccagtaactt ctatagtatc ttttttacca ttcatccctt gaactaaggc
    23701 atctactgtc ccttcaccac catcagcaag tggcaatatc tttacttgcg catcttcttt
    23761 aaccttcaat atcccctttt ttattgcatt tcctgcctct attgaagata agcttccttt
    23821 caaagaatct atagaaatta atattttcat atttatctac tcctttttat tactccttat
    23881 tttaaacata taattttata agcttaattt atcttttatt ttatactcta catctctctt
    23941 tgtatacaac ttataccatt aatattcttt tatcttataa aaagggagtg tctcaaaata
    24001 aatttcttag ttcatgagac actctttttg tataaaatca aatatatgtt tattatttca
    24061 acaatgataa ggaggattat ctctatttta agataattcc tttttagcac tatttaacca
    24121 gtatagtttt aacttggttc attaagcact ctacatttac agagttatta tctattgata
    24181 tagattctaa ttctttttca tttaggttaa tccatttagc tgttttaata tttttgttaa
    24241 atactatgtt agctgatttt gtttcttctt tagaattgta aaatctaact actaatgcct
    24301 cttgtttttc agcctttttt acaacactta acacaaggtc cttattagtt tccttaagta
    24361 aactatatga ataatctact tctatttctg attgattcaa tttcatagca ttatatggca
    24421 ttttattata agtttgtatt tttgttaaat actctttagc catatttgca acatttgctt
    24481 ctagtgtaga tttttcatga gtaactattg ctaagtctat ttttatatct cctatcattt
    24541 gagaatctgg agtaggcaac tttattcctg atggacgacc tgggcgtctt gccatttctt
    24601 ctttacctag gaatcctaca cttctaaata atgttatagc tattgtgtca aatttttccc
    24661 ctactatttc aaactctctt gtactgtttg taagaacagc tactccatgc tctttgtcag
    24721 acaatccaac gaatgttagc attggatata tagaatctgg tctttcactc cattcttcct
    24781 gttgccaaac ttccatagca tcatcataca cacttctctt tatatgtcca aactgattat
    24841 cagacacaga aaactttgaa gctatatttg ttggtatata agttctaacc ctacaatctc
    24901 ttgcttggtt atttatgttg aatactacat ctattatagc tttatcattt ggaacattta
    24961 taactatatc aaagtcaata aatgaatctg ctctattatt ttttctagct tgtatatctt
    25021 ttggtacttt taatctataa tttatcttta tctcattatt aaatctattt ttctttatag
    25081 tggaatttgc tactatatta tcgttaaata ttaatttctc atttggaagt ggtgaaaaat
    25141 catactcatc tccttcatct cctccatttt ccatcaataa tacattttca aaagttttat
    25201 ttaatttttt atcaaatatc tttaatgtcc cgttttcatt aacttgaata gtataaaagt
    25261 cattatctac catgtcacat acttcataag ctttttctaa catatgttca acttctttta
    25321 tcatatacgc cttatacccc attgcaggta ttttatcttt aaagtttatt gtatatacca
    25381 taaatgaatc atagtttcca taatgaacaa tttgacggtc tattaatcct gcatctatta
    25441 cttctttatg taccacttca aaatccaact tattattatc tttatcaaat atttcaaatg
    25501 ctttcatttt agttataact tgagcgctta ctatttcatt cctatcgtaa ggcattaagt
    25561 taaatgctac taacttatct aaagccattt cacaactcat tgcatcaact attcttcttt
    25621 tgtaaaatgt tattaattgg tctacttttt cctcagctaa gaaaaatcta ttcattattt
    25681 catggtgaac tttatctgaa caacaacatc caatgctgtc atgagcatga tttttcatta
    25741 tttctttcca aattaactct attaatccat gatgatattc aaatcctaga gaatatgcaa
    25801 tagaagctag tggctccaat atgttggtta tctttgattc tattctagca tttgctgatt
    25861 ttatatccat tcttgttgag aatatacttc tatgaaccct catatatttc ccatctaaaa
    25921 attccccttt tattgtatct atatcctcat ttttttctag ctctttaaat atgttttcat
    25981 atctacttaa gaaaaatttt ctctctggat aacattcttt taatttatat ataacttcaa
    26041 atatattctt ttgaattggc atttggtcat gtccatttgg aagtattata tggtcagtag
    26101 ttgcaccctt atcaagaacc ggtaaatatt tatccattct tgttcttagc tcatcttcat
    26161 tcattggtag atattttcct attgcataac ctaatggaag tatttgaacc aaaacagaag
    26221 acccatcatc actcttccaa ttaaactctg ttttattagt tccttttctt tcagaacatc
    26281 ctctccaaaa cattgagtat tttatatcaa acccatttag tatttgtggc atttgagcac
    26341 tttgaccaaa tgaatctggt aaatatccta ttttcatata ttctccaaat tcatcacaat
    26401 cttttattcc atatagtaaa tttctaagta tggattctcc accaactacc atttcatcag
    26461 tttgagtata ccaaggacct attattagcc ttccttcttg aactaatttt tttattctct
    26521 ccttacattc aggcttaact gctaaataat cttctaatat agcagtttga ccatccatca
    26581 cataatatgg atagttatga ttattttcta acatatccat tatttcttcc atattattta
    26641 caagtaatat tctagattcc tcagttgaga agtaccattc tctatcccaa tgcatatgag
    26701 gaactatgtg tacatttttt ttcattgctt atcctccaaa acttatctga taatattttc
    26761 tgtctaatta aaaatccaat aaaaatacta ctgagtttac aaaatttttt aatatttatt
    26821 ttatcactat aattaaacct ttgatttaaa tatctaataa cgtatctttt aatgtatctt
    26881 tctttgattt ttttgtttcc tagttattat taataatact gctgatatta tagctccaag
    26941 tactgccgca aagaaccata ttgatgctat aataaatcct gacatacttc cctctaaaag
    27001 cgccaatgag aatatccccg ctccaggcac ttgaagacct atattgaaga atgctactat
    27061 agacccagtt atggctgaac cagctaccaa tgatggtata acacggattg ggtcctctat
    27121 cataaatggt attgcacctt cagttattcc agccaatcct aatatccaag tttgctttcc
    27181 tatttccttt tctgattctg aaaataaatc tctgcctaaa acagtagcta ttgtaactcc
    27241 aaaagctgaa accatcttaa ctgatgcaaa tattgcataa ggtataaaat ttccacttgc
    27301 tattgctcct attgcaaatg tataggctgc tttatttatt ggtccaccta agtcaaatga
    27361 aaccatagct cctattattg cacctagtag tattgcattt actccagtca tcccatttaa
    27421 ccaatttata agaccattgt taagtgctgc tacaggcttt cctactacaa ataacatcaa
    27481 taaacctact accaaagaac caactactgg atatacccaa aaacttacga atccagctaa
    27541 tgtgcctttg gctggtatat tcttctttaa aaattttact atataaccag caagtaaacc
    27601 tcctaacata cctccaagaa atccactacc tattaaatca gctgctagac ctgctgcaaa
    27661 tcctggtcca agacctggtt tttcagctag tgagtaagac atatatgctg ataatactgg
    27721 aaccatcaat gtacctaaca tacctccaga aagctttctt aattgccata accaagaacc
    27781 ttcttgattg tatagttctt gtagaccaaa tgcttgagat gctaatactg caaatgcaag
    27841 tatcatacct cctgccacaa taagtggtat tatatgagat actcctgtaa gtattgctga
    27901 ttttacatct tttttccatg atgatttttc acaatatgat atatcagatg atttttctat
    27961 ataatcacct ttaccttgtt tttgtgctac ttctaaagct tcgtatatta tttcttttgc
    28021 tctttttagt ggagctgcta ctgacgtttt tatgtttggc aaatgttcaa atctttctgt
    28081 atttttaggt gctacatctg ttgcatatat tattgcatct gcccttttta ttatatctgg
    28141 tgtatgaggg tccaccatac cattagctcc ttgcttttct acatacacat ccacaccgat
    28201 ttctttacca gctttaatta atgcttctgc tgacatatat gtatgtgcta tacctgcagg
    28261 gcatgctgtt attgctaata taattttttt tgattcattt tgaatcctaa tctcttcttt
    28321 tatttgttct ttcttttctt ttatatctaa gttattaaat aactctttct tagtttttga
    28381 atttaacaac atattcatat aatcttcatt tgacagtcta gtaactaact cagaaagtat
    28441 ttgtatgtgt gtatttccac cttcattttc tggtactgct attaaaaata ttatctctac
    28501 tttattattt gggtctacgc tttcccaatt ttctattggt gtctctaagg tcatagctgc
    28561 aaatgcagct tctttaactg acttagattt tccatgagga attgcaagcc ctctttcaaa
    28621 acctgttgga gataaagttt ctctgtacat tacagcttct aaatattcct cataagatgt
    28681 taacttgcct tcatcatata gctttcttgc taagaacttt attgcgtctt tctttgtttt
    28741 tattttttgt tttaaaaaaa caaggttttc atttgtgatt ttcttaaatt ccaaaattac
    28801 gccctcctta aatcatttaa tgctatgaat atagcataaa ataaattatg aaaatgtttt
    28861 tttgaaatta tacacacaat ttcttcttaa aaaataaaga tttgtgtatt tatacacaaa
    28921 tctctatctt aaccagtatt gctctattat tgctctcata atcatcataa ttggaacctt
    28981 atctgatata ttgtacccat ttacttgttt aattgttgaa tagcaatata aacttatatc
    29041 tgcaatttgt tgcaattgat tttcacatat atgtgttaga gctactacct tacaaccctt
    29101 attaactgct gtctttgcta cttctataac ttctttcgtt tctccactta aactaagtgc
    29161 aaaaaacata tcttgttcac ctaattttcc tatatcatga agtacttcat gtctttgact
    29221 atagtaagtt atattatcat gcccacctct aatttctttt actaatatct cacataaagg
    29281 aatgttttga cctattccat aaactataat actcctagat ttttgaatta tatctgctac
    29341 tttatttatt ttttcataat ctattagctc taatgtcttt tttatatctg attcaaaacc
    29401 attttttggc tctttatcct cattttttaa ctcttctttt aaattatttt ttagttgaga
    29461 gaaaccatca tactccattt ttttacatag cctaactatg gtatttggga cagtaaaaaa
    29521 tttatctgct aaagattgta ttgataattt tactatttct tctttgtttt ttctcatata
    29581 ttcaattatc atgtcttctg tatctgtaaa tttatactca tatttttttg ctctttcata
    29641 aaattccata gtttctcctc catctatttt attattcata agtaaaattc aaaaatatta
    29701 gctaaaattt tcgttaatat aattataatt taatattttt aatttattaa tgcatactac
    29761 tctttttatt atacataaaa aatgtgtact tcctatattg atagagatga ttcaagtttt
    29821 tattttgata catctctatc aatatttttt tagaaaatac acattttttt caaatactta
    29881 tttttaatta aaactttagg taatctattc tttatatata attattagac ttgaaaatat
    29941 ctttaaaatc aaccaattca atattatttc tttttaacca cattttaact tccttatcac
    30001 atagcattgc atgttcatat actctctctt gaataagaga agatgatttt ataagactat
    30061 aatctataaa tcctggatga gtaaccatca atgttatatc tttatctaaa tttatatatt
    30121 tttcttcaaa taatttaaca aaagatttat ttaacttgta aaaatcattc tgaggatatt
    30181 tacttggcat gctaatacat gaatccaaat aatagggaca acttggtata ccgtattctt
    30241 ttgcagcatc acacattgct gatattgata catcttcttc tataccatgt atatcaatat
    30301 actcaggcaa tttactcata tacaacttgt aagtttcaat ttgagcacaa acttcttcat
    30361 atacttcttc ataattaaaa acctctttac ctgtcagtag ttgcgttctt cttattttgc
    30421 tagaaacaaa ttttccacct tcatctaaca aattctttat cttaatatgt tctttgcaac
    30481 aaggttctcc tattactaaa ttaacatgta aaccaaaaca tacatcatca ttttttacca
    30541 tttctatagc ttgtcttgaa aaaggcatat tcaccatgat tccagcactt gtaacaatac
    30601 cgttcttata agaagatatt atccctagtg taacaccttc tgaatatcct atatcatctg
    30661 ccctaactac tagtttcttc attttatgcg tctgcctgct ttctcaactc atcaagttgc
    30721 atttccatct catcctttac tttaacaaca tccgttccta tgatgatttg aactgatgta
    30781 ttactcaatt taattacccc tttggctcct gtttttttaa tattatcatc attcaaaata
    30841 gtagcatcct tcacttctaa acgtaatctc gtcatacaat tattaagagt cactatattt
    30901 tcataaccac cacaattttt aattatctgt ttagccatat atgaaaaatt accatgagaa
    30961 agttttaaat tcttttcttt ttcactaact ttatcaccta tctctatctc aacttcacgt
    31021 cctggtgttt gcacattatc ttttattatt atatatttaa atgttacata atataataca
    31081 aagaatacaa ctcctacagg tataactaac cacgctttat ctccataact aaaacttaag
    31141 acatagtcaa ttatacttgc tccattaaat gaacccaagc gaacacccag taaatatagt
    31201 acaactccac ctagaccaga ataaaaagaa tgaattatat ataactttgg agatgcaaat
    31261 aaaaacgaaa attctattgg ttcagttact gttgccataa aagaagtcaa tgctccagcg
    31321 gtcattgcac cttttacttc ttgcttcttt ttatcttgtg ctgttttata tatagcataa
    31381 gaaatagcag gtattgaaaa catcataatt atgtaaaatc ctgtccagaa gaatcctgca
    31441 tttgggtctc ctgccataaa tctaggaatt tctcctctta taacttctcc tgttgatgtt
    31501 gtatattcac ccatttcaaa taatatgtaa gcatttaaaa cttgatgtaa cccagttggt
    31561 attagtgcac ggttacagaa taaaaacaag aatattccta ttgcaccagc tcctcccaac
    31621 caaaaaccaa attgattcat tgcagcttgt ggatatatcc ataataaact aaatatatat
    31681 gataaaatta aagcaaccac tggtgatagt gtaattacac atttttctcc tgcaaagaag
    31741 cttaaaaatg ctggaacttt aactttatat gttttattgt aagtccatgc tatacaagct
    31801 ccaacaatta tcccaccaaa tacacctgca tttaatgtat cattcaataa ctctaaattt
    31861 gacttaaata tcatatatgc aactacggct gcaaccatta cacttgtctt gtctttagac
    31921 tttgagtagg cactagctgc tcctaataaa aataagtagt ccacatttga aaatactata
    31981 ttagatatat tagatatagc tggcacattg agcatatttg gaccaaataa taatcctaat
    32041 atacctgctg ctggcattac agctattggc actagcatag tctttcctaa aagtgacagt
    32101 tgttgcatca ttttgtttaa gaacctcttc atatccaatc ccctttctat ttcggtttaa
    32161 ttttttgtga aaacgattta ttcacatttt atattttaaa tattatattt gtttaaacaa
    32221 attgtcaata ttttttattc attttatttt tatcatttag tttaacatat aaatcctttt
    32281 aagtgtattg ctatatctag tctatttttt taattcattt tttttatttt aaaaatatgt
    32341 ttaaccatgt atttgaaaca acccacaaaa taattctatt ttgtcctttt tatggttgat
    32401 atgattctat tttcactata catgtctaaa cacatttgat ataatttgtt catatcagtt
    32461 caataatatt actaaagacc tttaaggagg gataggggta tatggctaaa aataaatttt
    32521 cagagatata tgaagtattg aaagaggaaa tacttgatgg taaatataca agtaatatga
    32581 tgctgcctac tgaacttcaa cttattgaaa ggttttcatg tagcagaaac actgtacgta
    32641 gagcaatttc tcaattaaac acagaaggat atgttcaaag tataaaaggc aagggtgttg
    32701 tagtgttaga aaatagttgt tctaatgatt tttttctaaa tatgcacaat ttcaaaggtg
    32761 tagaatcgat tgtagaagat aaaaaggtta atactgctac tagtgttctc cacttcagca
    32821 aaatcttaat tgataataag ttatctaaaa agacaggttt taaagttggt agtgaagtat
    32881 attaccttca cagactaaga tatattgata acatacctaa aattctcgac attaactatt
    32941 ttctatgtag tatagttaaa gaccttgatg tttctatagc acaaggctct atttataaat
    33001 atatagaaga gtgtctaggt acaaaaattg tttcatccag aaagatattt aaaattgaaa
    33061 aagctacaga gcttgagtta aaaacgctac ctctaaatga ttataattgt gttggtgtta
    33121 ttaaaaatag tgtatatact gatgatggaa agctttttga atatactgaa tcaaagcata
    33181 cacctgaaac atttgtattc atggatgtta ctcaaagata ttaaaaaaat tatgtttaat
    33241 taaacataat ttttttacgt ttagacactt tatcccatac taacctcact attctatata
    33301 caattatgtg tttatacaaa taattattta ttgtatattt tagctttaat attagtttaa
    33361 tgcttgacaa tttgtttaaa catattataa ttaacttata aattacagga ggtatcaatt
    33421 atgaaaaatt ggtggaaaaa agcaactgtc tatcaaatat atccaaaaag tttcaaagat
    33481 agcaacaatg atggtatagg tgatataaat ggtattatag aaaaattaga ttatctgtat
    33541 tatttaggtg ttgatttatt atggttgact cctatgtacg tttctcctca aagagataat
    33601 ggatatgata tagaagatta ttataatata gacccaaaat atgggacaat gagtgacttt
    33661 gaaaaactct taaaagaagc tcataaaaga gacataaaaa taatgatgga tatggtccta
    33721 aaccatacat ctactgaaca taaatggttt aaagaatcta aaaaaagcaa agacaatcca
    33781 tatcgtgatt attatttctg gaaagatgct aaacctgatg gttctgttcc taataactgg
    33841 atatccagat ttagtggaac tgcttggaaa tatgatgaaa ccactaacca atattattta
    33901 catttatttg aagaaactca agctgattta aattgggaaa atgaaaaggt tcgtgaagaa
    33961 tgttacaaaa tactagaatt ttgggctgat aaaggtattg atggttttcg tttagatgta
    34021 gtcaacctac tctccaaaac acctggatta cctgatgacc ctataacagg accaaaagga
    34081 gatggaagaa ctcactacgc tgatggtcct cgtatacacg aatatttaca taatatgaat
    34141 caaaaagttt tcaaacctaa aaatatagtt actgtaggtg aaatgtcatc tactacacct
    34201 gaagagtgta tcaactatac tagagaaaat cgagaagaac tatctatggt ttttagcttc
    34261 catcatatga aaacagatta taaaggaggc gcaaaatgga ctaatgaaat gttctctctg
    34321 gataaattaa aaaaagcaca atctgacttt caatataaaa tgtatgaagg aaaaggatgg
    34381 aatgctctat tttactctaa tcacgaccaa cctagagctt tatctagatt tggagatgat
    34441 agattttttc gtcaagaaag tgctaagatg ttagccataa cactatttgg tctacaaggt
    34501 actacataca tatatcaagg tgaagaaata ggtatgacaa atgcctactt tactaaaata
    34561 gatgaatatg atgatgctga aagtaagaat gcatactatc atatgattaa atctggtatt
    34621 gatgaaaagg aggcattgct tatacttcaa caaaaatcca gagataatgc ccgtactcct
    34681 atgcaatggg ataatactat ttataagggg ttttctaatc ataaaccgtg gttaaaagtt
    34741 aataatgaca atattagtgt agaaaatgag cttaatgatt ctcgttcagt attttatact
    34801 tatcaaagat taatcaaata tcgtaaacaa tatgatattt ttacagaagg tacttataga
    34861 ttgttagatg aaaatcataa aaatctattt gtttatgaaa gagagtataa aaaccaaaat
    34921 cttcttgtta taagtaattt cactcatgat aatgtagtat ataaattacc tgaaacttat
    34981 ttgaataaat taaactacac aatacttgaa accaattaca ctagagatat aattgaaaat
    35041 aaaatggaaa taaaacctta cgaaagtata atgatatatt ataaataaag ttatttatgt
    35101 aaaaataggt ataacaccct ggtgttatac caattttact aaattcaaag taatgtttat
    35161 agaatatcta tagattacaa taattatttg acttctacat cattatattt ctttgaatat
    35221 actttagtca tatagaaata tataggaact acaattatca tcccacctaa tccccaaact
    35281 atcttcatag gttcatcaat acctgctttt atcagtaata atatacttat aataactgca
    35341 aatattggta taattgctcc tccaggtatt ctaaaattta catctttacc agcatacttc
    35401 tttctcaata ttagtacagc taaacatgta ggaatatatt gaataaatct agatacaaca
    35461 cttatttgag ataatgttgt gaatgaaccc gaccatgcta ttagtaaagt tcctatgaca
    35521 gatactataa ttgctatata aggagctcca tttttattag tttgtcttat agacttaggc
    35581 attaatcctt gttcaaccaa agcagcagca gaccttggtg taaatatgga tgatgcaata
    35641 tttatacctc caacagatac tatagttcct attgtaatta tatacccacc tataggacct
    35701 aacatcttaa tggcagcatc tgcaacagga gtagctgacc ctgctaagcc attacccaac
    35761 acccctatac ttattcccag tataagcata tatattactg atactacaaa cattaccatt
    35821 accaatgctt taggtacatt tttcttaggg ttctccatat cttttgctgc aacagctaaa
    35881 gattcaaatc ctgtaaaagc ataaaataaa gttatagata ctgctacaaa agctgatgac
    35941 gcactttgac ctttttgtat tattacaaat ggggtaaaat tagaaccctt tataaagaat
    36001 aatcctacag ctataaataa aactattggt aataattttc ctatagtcat gatattattt
    36061 aagtgcttag attgtttcat tcccataaaa tttataatcc caagccctac aactaatata
    36121 ccaattatta tatttttaat aagtggttct gctgcttgtg gccatacaga tgatagtgtt
    36181 acagcaaaga aatttgccat agtagcccaa gcaatcatac taactatcca cttcataaat
    36241 ccaacttcaa atccaacaaa atctccaaat gcctcttttg cataaagata gggccctcca
    36301 tcagtatcaa accaactacc tgcttcagca aaacataaag caatagaaac agctaaaagc
    36361 atacaaaaaa ctaatactaa tatacttgct ggacctatat ctgtataagc atcaccaggt
    36421 aacccgaata tacctgaccc tattattgca ttgattccta atagtacaat actccaaaaa
    36481 cctaacttaa tgcctttttc cattgttatt ccccccaaag ccaacttaat atatttgtat
    36541 tttttattta gatgcttctt taactattgc cataaatatt tttttcatat tatcataatc
    36601 tctagtcatc atttctggat gccattgtat ccctattaca aactcacttc cttccttttc
    36661 tatagcttca attaaaccgt ctttagatgt agctgaaaca atatatcccg gtgctacttt
    36721 gttgacaact aagtgatgaa aactattaac taaaacttct ttttctccta gtatttcata
    36781 taattttgtc ccctccttta tctgtactgt atgtgtaggt acattagata gatgttgctg
    36841 attgtgttta atataagcac cttcaattaa agataaatct tgatacaaac tacctccttc
    36901 agttacattt ataatttgtt ctcctctaca tatacctaaa actggttttt tcatatctaa
    36961 agcatgtttt aataatttca tatcaaaact atctctcttt ggtgatattg cccccaactt
    37021 attatgaggt tcttctttcc atatgagtgg atttacatct tgtcccccag atagtataag
    37081 cgcatctata ttagatacct gctcctttat aatctcatca tcatatacaa tcggtacaat
    37141 atatggtata gctttacaca taactactga ttgtatataa tcattattta cataagccct
    37201 ttcatagcca ggaaacattc ctccttcatc aattagtaaa ttacctgata ttcctattat
    37261 tggatatttc tttttagcca atttcttcac cccttaactt atttgtgaac aataaaaaat
    37321 gctacttctc ttaaagagca agtagcatac caagatttta tttatttgaa cattataatt
    37381 tattttagaa atactttttt tgaatacgag taatataaat aactttattt acttatattt
    37441 tattttaatc ctgtttagtt tgactttttt aaattgtttt aagtacaact aataggtaaa
    37501 ataggcattt agattttcct ttttatcaac ctattaaata ctgtttaata tattttttcc
    37561 ccttctattt aattcacttc cctttctacc agattcacat attatcaaat ccagctcttt
    37621 taacatatta agtattcctc ttacctcacc ttctgttata ttgtagccta attcatttat
    37681 tgaattaaca ataccatttc tacctatatt tttattaaga ttattggcat tttttaaaca
    37741 ctccattact aactttactt tctctataga tgttctatcc tttaatatgc ttatttcttt
    37801 actaaaatct tcagttatat ttaataagtt atgaggtaaa tcatttatta aaataacatc
    37861 ctcagaactc ataatgttaa tatacatagc agtatttttt aattctctta tattacctgg
    37921 ccattcatat gcattaataa tgtcttgaac atcttgacta attgctctct tccttttcat
    37981 aaaatgcttt aacataactt ctatatcttc ttttcgctct cttaaaggtg gtatatttat
    38041 gggcaataca tttatcctat agtataaatc tgctctaaat ctatctcctt ttatcatttc
    38101 taataaattt ttatttgtag ccgcaattac tctaacatct atatctatta cattttctcc
    38161 tccaaccctc attacttgat tctcttgtaa tactctaagt aatttgcttt gtaagtatat
    38221 aggcatgtct cctatttcat ccaaaaatat agttccattg ttagcctgtt caaataatcc
    38281 ttttttacct ccttttaaag ctcctgtaaa agcaccttct tcatatccaa aaagttcact
    38341 ttcaagtaaa ttttctggca tagctgcaca gtttattgct ataaatggct gactatgtct
    38401 tggtgatgca ttatgaatag actgagccat gagttctttt cctgttccac tttctcccgt
    38461 tataagaatt gtataatcag attcagctat tttcatacct aatttcttag ttctttccat
    38521 cttcgttgaa actgttttta tatcatcaaa agtatatcta gcaacttgtc ccttttctct
    38581 aagcttcttt gataggtttt gctccaattt tcttatgtaa gttatttctt gaatacaaaa
    38641 ataatatcca gtaacccttc caaaactctc tatattatac ttatttacgt ttaagtactt
    38701 attattaaat tttataactt catcatgtgc cttactcaaa ctaaaaatct ttttaatatt
    38761 ctcattctct aattcatcta tatttttccc tataatatta ctttgaatat ctagtatatc
    38821 ccttaaacta ttattacaaa taattatgtt tcccttatct gataccattg ccatacctaa
    38881 gtttgataaa tttattatgg aatttagttc atctgctttt atagtaagtt ctttgtattt
    38941 agtatttatt cctgaatata aactaattat ttcatctgta tacttaatta gcctctcagt
    39001 aatttcttta tcatccagtt tgagttttgc tattatttga ataaacgttg aactatcaat
    39061 atacctatgg cctaaatcaa taacttcttc tatatatttt ggaactaaac ttgactctcc
    39121 tggcgttata gctatcttaa tatttctata ttcttcattg ggattgtatg gtataaaatt
    39181 caaatgattt attcctatat tgtataaatt agatattgtt tccaatacag tatgcttatt
    39241 atcgtttact actaatacat ctattccttc tggcaatgaa aataacttat atatatcctt
    39301 ttgctttatg ctcctattta tagttattat ctttgagaca tcttccacat atttttttat
    39361 ttttaatact ctatcatcta tcattaccaa tattatgtca tcttttatta attccttctc
    39421 tgttatttta tttaaataat aattttttat cattacttta tctttaaaga tcatttctag
    39481 atttttattt aagaaacatt ctaatttaga caacccatca gttactactg ctattgtttt
    39541 catttgatta ctccttttaa aatatactct actcttttat aatatacatt tgattttcac
    39601 tttaataaca caaaaaattg tacatttagt atactaacat attcctttat tagttttatg
    39661 tccataaata tcatagttgc aatatctatc atatttctta aacttctata aagcaaaaat
    39721 tgtgccaaat taataatttt cttaatttta ttctaaagtt cctcaatagt atttacagac
    39781 ttagcactta tagcttataa gtctttatat tattaatcta tttttatagg ttaattgtac
    39841 aaatcatata tcacttttac ctattaaatg cattataaaa aattgtattt ataaacacta
    39901 tttactactc ttctaagtac aaacagtatc tacatactat aacctttcac cattagattc
    39961 tattacttgt ttataccagt caaaactctt tttcttaatc ctagacaagt ctccacttcc
    40021 atcatcatac ttatccacgt atataaaccc atatctcttg gccatctcac cagtagatgc
    40081 acttactaaa tctatacatc cccatggagt atatccaaat aaatcaacgc catcttctac
    40141 agcttccttc atttgctcta tatgtgcttt taaatactct atacgatagt catcatttat
    40201 cactccatta gattcttttt tatcataagc tcctaaacca ttttcaacta ccatcatagg
    40261 tattctatat ctatcataaa tttcatttag tgcatatcta agcccttcag ggtctatttg
    40321 ccatccccat tcactagact taagatatgg atttttcact ccacctaata tgttgccact
    40381 tgtattatct tctttagaat cactacttac acatgtagac atataataac taaatgtata
    40441 aaaatctaca caaccttctt ttaaaatttc taaatctcct ttttccattt ttatttcaat
    40501 attattttct ccaaaatatc tattcatata agttggatat tcgccacgca cttgtacatc
    40561 tccacagaaa taattcatta ctctactatc tctttgagtt ttaactgcat catctggatt
    40621 acaagtataa ggatacattg ttgcaaatat ttgcatacat ccaacttgat aatttgaatc
    40681 tatttcatga gctaacttaa cagccttagc acttgctaca aattgatgat gtaacccttg
    40741 aaaacgaagt tgaggtatat ctacttggtt tataaaatct gtagtccctt catttaatat
    40801 tccttgtgat aagtagcctc ccataggcat tgtagctgaa tttatttcat taaatgtcag
    40861 ccaatatttt acttttcctt tatatctttt aaatattgtt tcacagtaat tcatatacaa
    40921 gtctattaca tctcttgaaa cccatccatt gtatttctct gttattgcaa atggtatttc
    40981 atagtgagat attgttatta atggttctat attatatttt aaacattcat ctatcacatt
    41041 ttcataaaat ttaagtcctg cttcatttgg ttctttctcc attccagttg gaaatattct
    41101 agtccatgct attgaaaatc taaatacctt aaatcccatt tctgcaaata aagctatatc
    41161 ttctttgtat ctgtgataaa aatctattgc ttcatgactt ggataaaatg ttcctttttc
    41221 tataactctc gttattcttt tacttacttt ttgagaacct ccagtacaca tatctgaaca
    41281 agaaggacct ttcccatcta aattccatgc tccttcacat tgatttgctg ctgttgctcc
    41341 accccataaa aaatcttttc taagtgccat aattctacct ccaataattt atttatagta
    41401 actttatttt aaaataaaaa tacatatata taattcttaa ttaatagaac tctcttttac
    41461 gctaaaacag ttatcaattt ctgactatta tttacatgct tttgtgtatt ttctacaata
    41521 tccacataat cagaataatt agtaacaact actggtgttt ctaagcaata tccttctttt
    41581 gaaatacttt ctatgtcaaa agttattaat gtctgacctt ttttaatctt atccccttga
    41641 ttaacaaaag atttgaaata tttaccttcc aactgaatag tatttaaccc tatatgtatc
    41701 aatagttcac aaccattatc agatataatt cctattgcat gttttgttgg aaataaagtc
    41761 attacagtcc catcaaatgg tgccactact tttccttctt taggtgttat tgctacacct
    41821 tttcccaata tacctgttga aaacgctgca tcttgtatct gctccaaagc ctttacttca
    41881 ccttttattg ggctatatac tacttctctt ttgttttttt ctataacgcc ttgcccatta
    41941 tcatctttag aaatttctac tatagcatca tctttccaaa aaaataatgt taaaacaaat
    42001 cctactacaa tagctactat tattgctatt atagttgcat aaacaccttt catatctcca
    42061 gttgttggat ttatataatt aggtatacca aatattccaa gtccacccat tacataactt
    42121 tttacattca ttatccccat tacagctcct gccacagcac cacctacaca actatatatg
    42181 aatggtgttt ttcttggtaa agttattcca tatattgcag gctctgttac accacatatc
    42241 cctgaaataa ctgcaggtat acacagctct tttatcttct tatcttttaa tttaaagtac
    42301 attgctgcta ctactgctgt ttgtgcaaaa gatggtacaa atactcccac taatattgtg
    42361 tcaaacccca tagtcataag gtttgacata gctattggta ctaatcccca atgtagacca
    42421 aatattacaa gcacttgcca caataaacca gttaatgcac cacataaaat tggtgaaaag
    42481 ttaaataaag ctaaaaatcc atttccaact atattagaag cataggtagc tataggccct
    42541 ataaccataa atcccaatgg taaagaaact aataaaactg ccattggtac aaagaaattc
    42601 tttactacat ctggtattat tttagcaaac actttctcca acttagctgc taataatact
    42661 attaaaatta ctggtactac tgttgaagtg tagttcattg ctattactgg taatcctaag
    42721 aaagttatat atactggtga ttcaaatatt gttccagtaa atagattgta taaaggttga
    42781 cctgtagatg ctagagtatc taattgaata cctgaatagc ataaaactgc acctataagc
    42841 attccaataa atggttttaa tttgaatttc ttagcagctg tatatccaag aattattggc
    42901 atatactgga acattgcatc tccaattgca tttaacataa tatatgcacc atcagtttct
    42961 ccatacaaac ccaatgctgt aaatactgca ttaaatcctt ttatcatacc agcagcacac
    43021 agaactccta atattggttg aaaaactcct gatattatgt ctataaactt attaagtaat
    43081 ccttttggcg catctgagtc tgaattatct attgtatctc ctcctaatcc agccaaatcg
    43141 cacacaactt cataaacatc tggaacatga ttaccaataa ctacttggta ttgacctgca
    43201 cttttcatta cagtaactat accatccatt ttttttagca catcatcatt tgctattgac
    43261 tcatctttta atttaaaacg tagcctcgtt atacagtgag ataaactgct aatattatcc
    43321 tttcctccta cattcgcaat gatttctttt gatagttgtt catactttct cattttttct
    43381 cctccatttt ctttttgaaa tatgaaggtt tggccaactt aatgtcacca cccaatatat
    43441 ataaacctaa ttattaggat ttatatccga cttaatactt ttacttacca ctctttcaac
    43501 gtgaatagtt aagtataatt tttcctcatc tgttaaatcg tagttgtatt ttttatgtat
    43561 aaattcatcc aacttttcta tgaatttata agcattttta tatttaactt ttattacatt
    43621 taataaatca tcttcattat tttcatgtaa attattacta acaattcgtt ctgcaaaata
    43681 ctttaagtga gtaataaatc tatgataata aactgaatct tcatcaaata ctattctgta
    43741 atggtattta accatatttg aaatttcttg tattatttgt gtaatctcat atatagtttt
    43801 tatactttga tccatttgtg cattaactat atgcagtgct ataaatccag cttcatcttc
    43861 tgatagtcta atttcaaatt ttttttctat ataatccaat gcctctaacc ctattttaaa
    43921 ttcactctta taaaatcttt ttatatccca aagtaataca ttttttacac ttactccttc
    43981 acgagctctt tctattgcag tatacatatg gtctgttaaa gaaatataaa tactatcatt
    44041 taatcttttc cctagttttt ctttagcata taaaattatt tcatctgata attccatata
    44101 tcttatagga atatctgcta ttagttcttt aaatttatca gatatatttg ggtctgaaag
    44161 tttgaatatc ttatcaattt tatcctttga tatatgctct cctgtacgtt ttttataagc
    44221 taaacctctc cccataacta ctatttcctt atgattttca tcagttgtaa taattacatt
    44281 attattgagt attttttcaa taatcattgt gatgccccct ttattagcat taagttaaaa
    44341 taaatctttt ttaattattt agaatattta aaatgtaatt tattacataa aaaaaaacct
    44401 atttattaca cattaaatag actcccatga gtcctgagta ataaataggt ttagcttgta
    44461 tttaaacaat cactatccgt ttgtatttta ttttaattat tatttattat atgttagcaa
    44521 atttattttt tagtgtcaac tttttctcaa tattttaatt ttaaataaat tatataaatt
    44581 gaatcttcaa agtactattt ttatcaataa acaaaataaa aaaggtatta ctttctttaa
    44641 gtaataccca tttatttcta atttaagtgg tttttcttaa tctcatcaag tagtccatct
    44701 tctaaataaa tttctaatgc acatcctacc attgccttta ctgctttgtg aagtttatca
    44761 taagcttcct cagaatttgc ataatttaga aattcttttt catgaacata taccattgga
    44821 cttatatcta aggctatttc tgtatacata gtagggcaaa cttgacttac attacctata
    44881 tctgtagaac ctactggacc atctttacct tctaatatat ctgttatacc tgcctcaatc
    44941 aaattattct ttgttatctt ttgaagtact ttgttattta ttatgttgtc aaaagaattt
    45001 tcaaagtatc tataagaaag cttagctcct gtcattaact ctgcaccttt tgcacagttt
    45061 ataacttttt tagttacttc atctaaataa cttctttcag cagctcttac gaaaaactta
    45121 caagctgctt tttctggaac tatatttgga gcctctcctc catttgaaac tataccatgt
    45181 attctaacat ctgattttac atgttgtctt agtgcattta tcccagcaaa agtaagttgt
    45241 actgcatcta gtgcattgac tcccatatga gggtgactag ctgcatgtgc tgatacccct
    45301 tcaaaactaa actctaatga atctatcgcc aaagccttac agtttatatt attaaaagct
    45361 tctatatgca tttgataact tacatcaaca tcatcaaaag ctcctgcctt aactaaatca
    45421 cattttcctc ctgttgtttc ttcagctggt gttcctatta gtacaatttt tcctttaaaa
    45481 ttttctttta acttacttaa tactatacaa gctcctagtg tagatgctgc aatccagtta
    45541 tgtccacaag catgagctgg ttctttattt tctccatatc caggaagtgc atcatactca
    45601 gctaaaaatg ctatggtagg tccatcatta tctcctaatt cagctctaaa agcagtctcc
    45661 atattacaat atggataaac tgtatcaaat ccatattcct tcattttttc tactaagtat
    45721 tttgatgata tgtattcttc ttcccctaat tcaggattgt taaatataaa attacaaact
    45781 tcttcaattt cttttccaag ttcattctct atctcataag ctctcttctt tatactctct
    45841 atcataattt acctcctact agaatcctag atataatcct attgtcaaaa atgccattac
    45901 aagaattgtt aatactacca ttaaaggaat tacgaatcta acccactttg accaaggaat
    45961 attggctact gctagtgctc ccattaaaac acctgatgta ggtgttatca agttagttac
    46021 acctatacca aattgataag ctgtaactat catttgtctt gatacatcta ctaagtcgcc
    46081 aagtggtgca agtacaggta ttgtaagtga tgcaagccct gatgctgatg gtattaaaaa
    46141 tgctattaat ccttctatta agaaagttaa atttataaat atagtttttg gtaatccatt
    46201 taataaattt gctgcagaat ttaaaattgt atctattata aatccatttt ctgctattat
    46261 tacaattcca cgtgcaaatg ctatacacaa tcctgctgtc attaaatctc ctgctccact
    46321 tataaatgaa ttaactatat catcttgttt aagacctgat gctatacctg atattatacc
    46381 tatagctgta aatatcattg caatttcagt tatataccat cccaacttta aaactccata
    46441 tatcataatc gccataccta ttgtgaaaat tgctattacc atggcttctc tttttgtaaa
    46501 ttcctttata tttgtatcac tattaaccat tgattcttga tttagtgata tatcgtgtac
    46561 taaagatttt ttagggtctt ttttaacttt attggcatac atcataacaa atcctataga
    46621 tatagccata tatattataa acataacaac tctaaactct attccagaac ctggtgctat
    46681 ttgtgataat gcttgtgcta ttcctacaga gaaaggattc gtagtagctg ctccaaatcc
    46741 agctgttgct cctataaata ctgtggccac tgctgtcaat gaatcatatc ccatactggt
    46801 cataagtgga ataaatatca tataaaaagg tagtgtctct tcactcattc caaacgtcgt
    46861 cccacctagt ccaaatagaa tcattgaaat cggtattatt aaaaattgaa acttcttcat
    46921 tttatttaca gtatgagcta taacagcttc tatagctcct gttttattaa ctatcccaaa
    46981 agctcctcca actattaaaa caaatccaac tacttcagca gcatctataa acccgtttat
    47041 aggtgctgta aatacatcag taattccttg tggatttgat ggtgcttttt cataagttcc
    47101 agctacaaca actgaccttc cgtctatatc tactctttca aaatttccac ttggcactat
    47161 ccaagtaaga attgccatta acactattag caaaaatatt attgtaagtg tctgtggcat
    47221 agtaaacttt ttctttttta aactgctcat ttttaatctc cccacttttt aaattaaata
    47281 ttttattatt gcatcccttt atcctatttt gtatagatgt aaaaaagctc aacatggata
    47341 catgttgagc taaatgtgct aaatacaaca tacctgcatc ttccctttat cttgtttaag
    47401 atagctctcc atcaaaattc gggataattt tgatgacagt actgtaccta ttaaatacag
    47461 tcccagcact agcttcggac actagcactt cggcacttac tccttttata ttgcctcatt
    47521 ggtttccaac ctcttcaata ttaatcttag taaatgcaac ctctacctca atctgtttgt
    47581 tagatgaggt aattcttatt aatttttatt aattcaaagt aattgtagca taatatgtcg
    47641 agattgtaaa tattttcagt ataataattc ttactttatg atttcattcc atttttcaca
    47701 aataattttt tctccatata atttaaatac tgtagtatat tttaaataca cacaaacttt
    47761 ccacaaattt taaattatta taataccttt ataataaaaa ataaaagcta tgtaaactta
    47821 tcaatttaca cagctttata tacattaatc tactccaagt acagctttaa gctttgatgc
    47881 tattgtaata actcgtggac caaatataac ttgaactgag tttttctttt taacaactcc
    47941 acttgcaccc aattcattta cccagaagcc atcttcatag aatccatcaa ttgaatttag
    48001 ctctacccta agtctagttg cacagtttgt aacatttttt atattattac ttcctcctaa
    48061 accttctatt atagaatgta caagctttgt atctttatca tctacttcct cttctactgc
    48121 attgttgtat gctgttttac cagcatctac agattgtctt tctctatatt ctttcttact
    48181 ataaagctta acttcttctg aacttcttcc tggtgttttt atatcaaact tcttgattgc
    48241 ccatgtaaat attatataag taactataaa aaatattggt atcaaaaata ataatggtaa
    48301 cgcatccact ttttgtggtt gtaataaatt aggaaacata tttctaaatg attctcccat
    48361 tatagaaaca ttaaacattt ctgtaaatac atacgtcaat cctgccagtg gtacatatgc
    48421 tacaaaatac aataatggtg caacaaatag gaatgtatac tctattggtt ccgttacccc
    48481 taccaacatg gctgttacta ttgctggtat caatatactt attgcttttt tcttattagc
    48541 attatcagcc aacttataca tagctaaagc tgctgctggt aatattgcaa aatgtattaa
    48601 taaacgacct gttgtgaagt tacgagttat ataagatagt gtatctgggt ctcccatttg
    48661 agctaatctt atattattaa caccctcgta aattgctcca cctacttcca ttgttccacc
    48721 tactcttgta tattctatag ggaatgcaat taagtgatgt ataccaaatg gtaataatgc
    48781 tttatccatt gctccaaaag taaatgttcc taataatcca ctagaaccta tccaattacc
    48841 aagtttagat aatgccaacc ctactggtgg ccatataaag tacattgctg tacccaatac
    48901 tattgatgct ataaacatca taatcattga aaatcttgga cctgagaaaa aagataatgc
    48961 actaggaagt tctatgtcaa caaatttgtt atgtactgca ctagctacaa atccagctat
    49021 tatagctcca aatatactac aatcatatga aaagattcct aagaaagaac catataaaga
    49081 acttcgacta tatgcttctg cctctcctaa tccagatgcc ataaacgctt ctgctgtaac
    49141 tgtatctgaa tttataccat ttgctgctaa caatgtactt ataactgtgt gcattcctat
    49201 aaataaaaca attcctccta atgcacccca tcctttttcc tttttagata atccaaaggc
    49261 tatacctgta acaaagaata ttggtaagta tctcattacc ataaatccta aatcgtttat
    49321 tactttaaag aatacattta taaatgaccc ttcttttatt agtccgctaa gccctaatgc
    49381 agaaacgttt tccatatttg caaaagctga ccctagtcct ataaatactc ctgctacaac
    49441 aagtaatata acaggtatca tcattgctcc agaaagtact tgtaatttat ccttcatagt
    49501 attatttcct ccatttttat tgaaatgcca ggctatataa tgcatatata tatacttcta
    49561 ttgctttgta taatgtatct atacttatat tttcatttgc ctgatgtgaa tttggattat
    49621 ccccctctat tgtgggtcca aatgcaactc cattttttaa cactcttgca taagatgcag
    49681 ctccttttgt aaaaagttcg gcttcttttc caaaaccttg tttatatgca tttgataatt
    49741 tatttattaa ttcactatct ggttttacat acagtaattt taaatcctta tatatatcaa
    49801 ctattaaatt ttcttttttt ccaatttcat taattctatt agttataaac tcccaactta
    49861 tacccttagg atatctaaag tctattttca tattcaattc atttttctct aagaagatac
    49921 tcattataca tattgtagaa actcccatat ctacatcttc tttatatatc cctaattttt
    49981 ttccatgatt atcatctgta aagtatgaat ttaaaatatc ccttattata acgcctttat
    50041 catttagttt tttaattttc tctaaatctt ttgctaaact aaatgcacag ttatatgacc
    50101 tgtgtggatt tctcgataaa gccctttctc ctgtatatct tactaatact ttaccttctt
    50161 ctagttcttc tatctctgta taataagcta atgattctac taaatctttc ttatcattag
    50221 ttttaaaaac agcctcaatt ttgtcacata caaatccatc ttctttgttt gactttatat
    50281 caactaaatt gtgatttcta aatttatctt tatctatttt ctttcttaga ttgaagtata
    50341 aaataccttt ttctccattt actattggga agtttccatc tggagaaaat gcaaattttg
    50401 gctgttcatt gacactaaag taatgctcca tacattccca agtcgtctct tcagctccac
    50461 ccactatcaa tctaatcttt ctcttcggct tttcattaag ttctcttaag aaaagtaatg
    50521 catataaaat ccctatcaaa ggtcctttgt tgtcattaac tcctctacca taaagatagt
    50581 tatctttttg acatacttta aaaggtttac tcttccaaag ttctttatta tatattggca
    50641 ccacatctat atgattaagc acccctacta cttcttctcc ttctccatat tctatatgaa
    50701 ttgcatatcc atcaaagtct tttacaacaa aatctttgtc ttttgctatt tgaataagct
    50761 tatcgaaggc atttcttatt tctatcccaa atggttgatt tatatctgtt gtactttcat
    50821 ctctaattga aggtatttca actaaacttc taatatcatt tatgatattt tcttgattgg
    50881 attttaaata atcaataatt agttctttgt ttactatcat cttaattatt tatcacccct
    50941 ttatattaat tttacaaagc atttcagttc atatctacat tataaatttt gaatattatt
    51001 ttttcaatat ttcaaatact tctctaagtt accataatga tacggtaaac aatcaacaga
    51061 ttatgacaaa atcatactat acctattact aatattttaa cttatctatt acgtcttttt
    51121 taatgtaagt ttattccaaa aatacataaa aaaagatgta gccttagcta caccttctta
    51181 tatctattca acttccatta ttatgctatt aatatctaca tttccaggtt ctatcctatc
    51241 ttttactgtt ctcttaacag aatcagcatc atccattata actacaggag ttattatatt
    51301 taaatcattt gacgttataa aatctaaatc tacttccatt attaaatctt tagcttttac
    51361 cttatctcct gcctttacct taacatcaaa cggctttcct tctagtgaca ttgtctctaa
    51421 acctacatgt attaatatat ttgtattatc attactctta atcattattg catgctttgt
    51481 atcaaataca ttgactattt caccatcaac aggagaatat aacttacctt cacttggtat
    51541 tattgctact ccatctccca tcatcttttg ggcaaaaact gcatctggaa catcttctaa
    51601 gttcactaaa tttcccttaa aagggctata tattttctca atcttatttt cttttttctt
    51661 aaatatatta aacataacac actctccaca ttataaaatt ttatgataat atttttctta
    51721 aagcttttgc tacaccatca tttacattag tatcagttac aaaatctgcc atagatttta
    51781 cttcatctgt agcatttccc atagcaatac caagacctgc aaatttaatc atggatatat
    51841 cattttcatt atcacctata caaataactt ctttgctatc tatacctaat atatcacaaa
    51901 attttttaac tgcaatacct tttgaagtac ctttactcat aacttcaaag tttatctttc
    51961 cagaacttac tacttctaaa tcttcttgtt ttttaaattc ttcctttgct tctctcaaag
    52021 aatctacgtt ttctgagaaa agtatacatt ttaaaatttc attatcagct ttttctaata
    52081 aagtttcaca acttgcattt tctattattt caattctgtc ttcttcttta tttttactat
    52141 ttattaatct atatggatgt tcttcaccta tttcattgtt ggatataact attttacagc
    52201 ctttaaaatg tttgcttaaa ttatactttt cacctattgt atagattttc tttagagctt
    52261 ctttgctcaa aaccttctta tactcatatc ccagtaattt aaaatatgtc ccattagttg
    52321 atataatata tatatcatcc cctaaaacac ttgtaaaata tttcatagca ttataaggtc
    52381 ttcccgtaac taaggctacc ttgataccct tttccatgac ttcttttagt gctttaatat
    52441 tttcttctga tacttcaaat cctttaccca tcaaagtacc atccatatca gtacaaatta
    52501 atttatatcg catttgtatc acatcctagt aagatttttc taatatttca acaaggtctt
    52561 ctttctttcc aaaaggtaaa aatcctaaaa ctggacagct tgttatttgt tctaattgtt
    52621 cctttgttac attaaactca gacatttttg tcttaagtcc cactctcttt aaaaaacttt
    52681 ctatttcatt tcctaaagtt ttagctgctt catcatcttc cactttttct aattcttggt
    52741 ttagcattct cgctactttg gcaaatttct ctatattatc cttatagaac ttttctgcaa
    52801 attttgggaa cactatagcc aaagcctcac catgaggaat atgtgtaact cctcctatta
    52861 tttcacttag tggatgtggt gctgctgctc cagcatttgc cagtgaactt cctcctaatg
    52921 tatctgctac tgctaacttt gttctgtatt ctatattatt tttatcttcc attactttag
    52981 gtagatagtt aatgactaat tccatagatt tttcagacat tatctctgta aaatcattag
    53041 catttggatt tatataactt tcaaatgcat gagtaaaagc atcaaatgct gttgatgcag
    53101 taattctctc tggcagagtt aacataagct ctgggtcaac tatacattct tttgagaaac
    53161 aattttgatg aaatattgta tttttctcgc ttcctctcga tattactgct gcttgtgtaa
    53221 cttggcttcc tgttccagaa gtagttgata cagctattaa tggtaattct ttttcactta
    53281 ccttatcgta tattttaaat ggatttgtat atgtactaaa catataatcc caatctatct
    53341 tttctaaacc aaaagtcaat gctaatactt ttgcagtatc tatacttgaa cctcctccaa
    53401 cagctaaaac aacatctaca gattcttttt tagctaattc aaacccttca tttattaatt
    53461 ctacagttgg gtttggctct actctatcaa agtgtacaac ttctatacct ttttctatta
    53521 aattcaatgt aactctttta tataacttgt ctaatggctc tacatttgga gtagtaacta
    53581 acaaacattt ctttccatat tttcctatta ttctaggtaa ttcacttaaa gttccttgtc
    53641 caaaatttat tcttgatggt tgataaaagt taatcatatt tttcctccct taaattattg
    53701 ccttcatatt caaattatac atatcatagg atttttaagg caataccttc actgttaact
    53761 cttatttact aaagcttatg gcaattatta gacaatcaat gttttattat taccatatca
    53821 ttatgtaaat tttattaagt acccatgtta taattttata atcaatctta ttgtataatt
    53881 gacttaataa taaaaataga gtatatacac ttttttatga caaatagctt aaaggagaat
    53941 taaattatga aatatttgat agacagtgct aatcttgatg aaattcaaaa gtgtttaaag
    54001 tatggattta agggaataac agctaatcca tctatgtatc ttaaagagaa agtaaacttc
    54061 catgaattta ttgagacatg tgctaattta aatccagaaa tattaactgc tgaagtcatt
    54121 ggaaataatt tatacgaatt aattaattca tgtgataaga tattaaatat aaataaaaac
    54181 actataataa aaataaattt ctctgaagtt ggcttagaac ttataaacat attaaatagt
    54241 aaaggtgtaa aaactgcatg taccttgatt ttcaatgtaa atcaagcatc attagctata
    54301 aatgcaggtg ctgattacct attccctttt ataggtagaa atgatgaaaa tgggaatgat
    54361 ggtataaaaa accttacaga aatatgtaac cttatatcaa taaatggata taatactaaa
    54421 gttgttgcag cttctattaa aaatgtattc cacttaactc aagcatctct ttgtggatgt
    54481 gattatgcag ctgtaacata tgatttattt aaaaaagctt cttttaacca gcttacaata
    54541 gatggtgcca atacttttga aagagattgg tccaaggtta ataaatagta actaagattt
    54601 aggagggttt gcattgaaca aaagattatt agatattgct gaattattac tcaacagctc
    54661 agactatatt actgtagata ctattgccga agaattaaaa gttagtaata aaactattcg
    54721 taatgacctt gttattttag atgaatggct tttagagttt aatttatccc tagataaaaa
    54781 aactggttca ggggttatta ttctaggtaa tgaagatata aaacttaaag ttattagaga
    54841 tatcaatgat aaatcaaatt gtatatatgc atactctcct gaagatagaa aaaggtatat
    54901 tctaagtaaa ttatttatta aaaattctaa gtttaggatt cgtgatattt gcaatgaact
    54961 acatgttagt agagctactg tgcataaaga cttggtagtt attcaaaatt ttttgcaaaa
    55021 ctttaaagtg actctagtta gaaaaaccaa taatggagta tatttagagg gaaaagagaa
    55081 agatattcga aaagcaattt ttgaacttgc tacggttaat aaaagttata ctgaacttaa
    55141 agaaatatta ttctcgaaat ctaatgaatg caaagatacc ccttcaacaa aaatatttaa
    55201 ggagctattt aactatgatt ttgagaaatt atctcaaatc actctacaca ccttacaagt
    55261 agaaaaaagt aaccttagtg atgagtatta tataaacttc ttaatacata tagctatatg
    55321 tattaaaaga atttcaatta aaagatatat taacttatct gaaggttttt ttagagaatt
    55381 attagaacat gaatactttg aaaaatcaaa aattttatgt gctaacctaa gtgaagcttt
    55441 tgacgtagaa tttattgatg aagaggtctg ttatatcctt cttcatatag aaggattact
    55501 taaatcaata aagaaaacta ctactcttga acctcttaaa aatgaagaaa ttacagagta
    55561 taatctatct gaagcaatag ctgttcattg gggaagtata ctaaatttaa acttaaagga
    55621 tgatttaata ttaataaagt ctcttgctaa tcaccttaaa tcagttctac acagaataaa
    55681 ctatgggttt actattacaa atcccatatt aaatgatata aagagaacat ttccttatac
    55741 ttataaggct gctaaagagt ctaatactgt tatcaaacag ttgatgaatt atgaagtcaa
    55801 tgatgacgaa ataggatatc ttgctttata tttaatatca gcaatagata gaagcaaatc
    55861 tccccttaat actattttaa tatgtcactg tagtgatggt gtatctaatt tattagtcca
    55921 aaagttaagt tttgaattta atcagattaa tatagttaaa tctatacctc tatcttctat
    55981 ttcatttacg aactttgatg atgtggactt aatactaacc actgctcctg tagactttga
    56041 acataatgct gaactgataa atataaatgc tcttttaagt aagaatgata tttctagact
    56101 tagtacaatt ataaaaaaac tatattctga aaaaaataaa atattatctt ataaataaac
    56161 aaaaataggc ttgtctcaac atagagataa acctattttt cattgtctaa atgataaaaa
    56221 tatatttgat ttcatagaaa aagtgcctca tgaactaaaa aattcatttt gagacacctc
    56281 cattttttat atttaagctt tttatttaag cttttatatt ttttaaactt ttatatttaa
    56341 agtaaatttt attattttta ttgagcacaa gcattattta tattattaat ttcttcagta
    56401 ttcttaccaa actttgtagt tacaaatcct attgttttta ttatgagtaa taaagatatc
    56461 aatgaatata tagatatcat aaaattctta cttattattc cagacgctac tattataaat
    56521 ccatctagca taaataatat aactggcaac ttcagaaact taattacctt attaataagc
    56581 attgcaacta aatcagttcc accagtacta cagcccctta ttattgctat ccctatagca
    56641 gttcctacca ataacccacc tatcactcct gctactaata gattgtcagt tatgtgagtt
    56701 tgtggtatta cttttaagaa taatgggccc ataagagtta tatataatgt ttttattcca
    56761 aaacctttcc ctaaaaataa taatcccatt actaaaagtg gcatagatat tcctaatgat
    56821 atattctcaa caggcattga taagaatgtg ctaagtacga ctgataatcc tgttatccct
    56881 cctggagcta atgtatgttc tgcaaagaaa aaattaactg ctaatgccat taaaaatgct
    56941 gatattgtta tcagtaaata atcttttatc atttcttttt tcatttaata acctcctatg
    57001 attatattat ttcagtataa aaaatcaatt aaattagtct agcttcctgt agtttttcat
    57061 aaaattaatg cttccttaaa acattaagaa agcatctctt ttatcatagt ttattatctt
    57121 attctaaacc aaactttgta gctgctttat ctatagcttt tctacaattc ttaacacctg
    57181 ctgttatacc atctttatcc ttgaatattg ctgaagataa tattacaacg tctggattac
    57241 attctattaa tggctccata ttgtctgctc ttagtccacc atctattgct aattcacact
    57301 taggattctt ttcgtctatc atttttctag ctctttttac taaatcaaca gcactctttc
    57361 tccatcccca gttatctttt ccatcagttt catcaacacc atgaacgact atttgcaatc
    57421 tatctatatc atatatagct tcatcaacaa aacataatgg tgtgtaacat ccaattgtta
    57481 atcctacttt cataccaaat tctctacagt aatttattat gtatgcaaga ggagcaccta
    57541 aaaaatgttc tgctggtatt atcaacaagt tacatcctgc tgctgctatt ttttctataa
    57601 ataatctatc acaatctctt gtgtatatat ggcattctat tggtttatct gtcactggtc
    57661 ttattgcttc tattatttga tgaccaccca taagttgcat attttttaag tcatgcatat
    57721 ctgctgcatc tgaatgcacg tagtctacac ctgcatccat tgcttcttta actacggctg
    57781 atatatgacc gtaatttaca tgtgctaatc ctgctgctat ttttatgtgt ttcatatttg
    57841 tatacctcca tattttactt taattagtat taaatattag tttttgatat ttttttaata
    57901 ttttttaatc cagtattact tttatttagc attactttta ttgctgtata tatttctttt
    57961 atgaacttat tctgcttttg ttaactgtga acttattttg ctttgtaaat taatattaca
    58021 ctgatatatt ttttttcaca acactttgac tactccttaa aagtaccttt atattgtggt
    58081 aaattataga cattaattgt atataaaaag ttagtttttt accacattct tatggcattt
    58141 attgtctata ctaactcaat tgatatagct agttcattta atatataatc agactataaa
    58201 tataaattta ctcgataaaa agtaccattc ataacaatat taatgaaagg gagatgtaaa
    58261 atgagaaact gtattattgc ccaatctgga ggccctacag ctgttatcaa tgcttctgca
    58321 gttggtattt atgaaaaaaa tttagaaact aaatactttg ataaagtata ttttggtatc
    58381 aatgggattg aaggtatatt aaacaaaaat ataatagatt cagactcctt aaacaaagat
    58441 aatgtattaa atttaaagca aacaccttcg tctggtcttg gttcttgtag atacaaactg
    58501 gctgattata aaataaattc ttatgattat gatagagtta taaatatact agatgaatat
    58561 gaaatagata cattatttta tataggcgga aatgattcta tggacacagt gatgaaattt
    58621 tctcaatact gcaaaaacac tacttgtaaa ataaaattta taggtattcc taaaacaata
    58681 gataatgatt tattaggaac tgacaactgt cctggatttg gttctgctgc aaagcttatt
    58741 tctacactaa taatggaaac atacttagat ttttctatat acaaaaataa tggtatattt
    58801 atagtagaaa ctatgggtag agatactgga tggttagcag cttcttcttc actagctaga
    58861 cttaatgata ctccacttgt tgatttaata taccttccag aaattgattt ctatgaagat
    58921 aagtttatag aagatgttag aaaaatattt aaagaaaaaa ataaggctta tatagttgcg
    58981 tcagaaggta taagggataa atacggcaac tttatatgcg aacaaaaaac ttctgctcat
    59041 gataaatttg gtcattccca attaggtgga gtaagcaact atctaaagaa tcttatagta
    59101 tcatcatcaa ttacaacaag agtaaaaacc ttagaacttg gtgtaataca aagatgtgct
    59161 atgcatttag cttcagatac tgatttaaaa caagcagctg aagttggtgc atatgggata
    59221 gaactatcta aaaaaggata tacaggagtt atggctatca ttaatagaac atgcaataat
    59281 ccttataagt atgaagttag tcatactgat atttctaaaa tagctaacaa gacaaaatat
    59341 tttcctaaag agtggataaa tgaagaatgt aattatataa ctgatgaagc cctaaattat
    59401 ctatatccac ttattcaagg taaaccagat atcaaatata aaaatggact accttatttt
    59461 tcaattattt actaggaaat tattataatt tgatatacac tcaattctat cgctcttaaa
    59521 tacctaagtg gctgttaaat aaaattataa attgttatat gacagtcact taatcgttta
    59581 attatttaac tgtttagttt tttaattttt taatcttttt taatactaat acgatttgtt
    59641 gtaataatat ctcacaccca ttttcatttt ggatagatta taattagtta gacaataaat
    59701 attttgtcat gatattatta tatatagtga tacaaataga aatactttta tcatggctag
    59761 aaaaacaaag attacacaaa ggatttaaaa atcaaacaac ttcaattaaa ataaatggtt
    59821 aaaataactt ctaaatcaac tactataaac aataaaagaa cataaagtag ttaagtctac
    59881 aattataaaa ttagtaagac tataataact taaataaaat taaagttaac aaagactatt
    59941 aaagttagca aatattcctc tacctaaata attgcttttt ataaagagtt atttatttta
    60001 attgaattta attgaaatac gattttaact agaacaatag attaatgtaa tatttaatat
    60061 aaaataatta taataattga aaaggaagta atctatgatg aaaatagcag tcataacaac
    60121 cgaattttta aaagagtttg tagataattc tataaaaaag ttaaatatta atgcagaaat
    60181 tgaaatatat atctatagag atttttctca tgttggagat ttatatttag aaatagaaga
    60241 taggtttgat ggatttgcag ttagtggccc catccccaaa aaagctatta caaaaaaggc
    60301 tggaactatt aaaaaacctc tagttgattt tggtactgac ttacaaagtt actatgatat
    60361 gtttctaaag cttatcttta aatataatac attagatttt tctagagcgt actttgactt
    60421 aacagactgg gcagataatc caaaagatat atcatattat ttatcaaatg gcacatttgg
    60481 agacttaatg gatagtattg atgaaaatgc atctaaaatg agtttatctg acatagcaaa
    60541 acttgaaaaa aagattgcaa aaaaacatat taatctatgg aatgaaggga aaatcgattt
    60601 ttctactact cgttttagta gtattgttcc tatgttacaa aatgcaggag taaactttca
    60661 tttcatatac cctgatatag atttattaaa aaatactttt aataacttaa taaaagatat
    60721 tcatttacat agaatgaagg gaaatcaacc aagtgtaata tatataacag ttaaagatac
    60781 aaattatgat gactctactc tagaccaagt ttataaaata ttaaatagta ttaaaaaaga
    60841 taaattaacc gattttataa ttgaaaaaaa tagtaattct tttaaaattt tcactaaatg
    60901 tgaaacgatt aaatcattaa ctaaaaattt tactgcatgt tatataaaag aatctattga
    60961 aaatagtaca aatattaaag tatgtgttgg atatggaatt ggtaatgata taaagcaagc
    61021 tatgtctagt gccataagtg ctaataagga atctaaaata aatcatttaa attacagttt
    61081 cttagtcaac gaaaattatg agacaatagg acctttactg tgtgataagt tacttactgt
    61141 ttcaaataaa gtaactccac atataaatac aattgccaaa agttctaaat tgtctactct
    61201 tactattcaa aagattatat ctgttactga aattctacaa agagatgaag ttactcctaa
    61261 ggaattatct tcatgtttaa atgtaacagt tagaactgct aatagatttc taagtagtct
    61321 cttaaagtct ggaaacgcta ctatattata tgaaaaacaa aactcaacaa aaggtagacc
    61381 tgaaagagta tacaaattat ttatatcaga aaataaatat taaaatccaa aatgcataaa
    61441 aatattttat catctaatat taagtaaaat tatatatgac tttctaaaag gtatatcttt
    61501 gcatcttttt aagatatacc ttttataata tttaataata ttttatgtta ttatctacta
    61561 aaattatttt ctaaccattt tactgcacaa tgagcaaata aagctgaacc ataaactaat
    61621 ccctcttcat taaaaattgc tttaggatta tgcataccaa aattatatcc ctgactaata
    61681 cttcccattc caatccataa aaatactcct ggaaccctta gtaaaatctc tgaaaaatct
    61741 tctgaaccca ttgcagatgg aaagtctatt acattttcat ttcctataac ttctttaaca
    61801 taagatacaa tctcatctcc tacatccaaa tcatttataa ctgcaggagt ccccatttta
    61861 aattccactt tagcatttcc tccaaatgat ttagctattc ctgtagatat ttcaataatt
    61921 ctatttttta atttttctct aacagattgg tcaaatgttc ttaaagtacc tgacatagat
    61981 gctttatttg gtatagcatt tggtacgctt cctgcattaa acataccaat agttaaaact
    62041 acaggctctt tctgaggttt ttctcttgaa agtatagtgt ttaatgctat ttgtatatgt
    62101 gacattatgt ttattgggtc cactccaaat tcagggcttg ctccatgagt tgcttttcct
    62161 tctactgtta tttcaaatat atctgcagat gccatagctg gaccacgtga ataagctact
    62221 tgacctgtgt taagaaattt acccaccata ctgtgaaatg ccatagctgc atcgacctga
    62281 ggattttcta atataccatc atcaatcatt gattttgcac ctctggtagt ctcttcatct
    62341 ggttcaaata caaactttac tctaccttta agtaagtgct tattatccat aagtatttta
    62401 gctgctccta agagcattgc tgtgtgatta tcgtgtccac aactatgaga tgcatcttta
    62461 ttcttacttt taaaaggtaa atccgtctcc tccataatag gaagtgcatc catatctgcc
    62521 cttaataata aggtctttcc accattttta ctgtctaaaa cagctactat tccattatta
    62581 gacgtcaatt tcggagaata tcccattttt tctaaagtac tggtaacaaa ggttactgtt
    62641 ttcggtagat tgcaaccaat ttcgggattt tcatgtaaat atcttctcca tctaataatt
    62701 tccccttcta ttgactgcgc ttctttaaaa aaatttttca tattccttcc tcctaatcag
    62761 atagtcagca aaatctcact ttcttttagt aatgaaatga tgctgatata aaaattataa
    62821 ttttatatat taaatttcat gtctttttaa ggagttggaa tatcatctgg aataggacat
    62881 ttatatgttt taccattcat agatttatca aattcttctt ttgccttttt agctatctct
    62941 ggattattta atattttagc tccaaacata gccataatct ttgatgcata tatcatacct
    63001 ttttctccta tagaacttcc tgcacaagat gtaaactgcc agctatgacc tggtgctcct
    63061 agattcgtac accctgtaaa aaagtatgct gtcggaacta tatgcattac atctccaata
    63121 tcagtagagc cataactatt aaagtttgta acatttcctc caccataata tagatgagtt
    63181 ccttctggca aattatattt acttatcata gccttatatt gttgtgggct ttgtctatct
    63241 aattcagctg caaaatccaa ttcctctcga gtccaaggct cttgtttaat ctcattcata
    63301 cattctgata caagatttgc taaaacatga ttgtttaaag tattataaca ccctcctaaa
    63361 aactctactt caacttcagt ttcagtcatc attgcagctc cttttgctac ctttacaagt
    63421 ctttcatatg tactttctac agcttctcta cttaaagctc ttgtataata ccatactgat
    63481 gctttatcag gaactatgtt gggtgctact cctccatctg taattgtata atgaatcctt
    63541 acatcagaag taacatgttc tctcaaataa ttagctccca cattagtaag ttccacagca
    63601 tctagtgcac ttcttccatt ttgagggtct gagcctgcat gtgcagtctt tcccttaaag
    63661 tgaaacttta cagagttaac agctgttgaa gtaccaattg tagcttcact aattttattt
    63721 ggatgaaaat ttatagctaa gtctaaccct tcaaacgctc cacctctagc cataaatcct
    63781 ttacctgtta attgttcttc tcctggacat gcataaaata ctatagtccc acttaagtta
    63841 ttttctatca tctctctttt aagccctact actgccccta catgtgctgt acataataaa
    63901 ttatgcccac atccatgacc atatgcaccc aattcaaatg cttcttgttt tgttgaaact
    63961 ttttgattta aaccaggtaa tgcatcaaat tctcccataa aacctataac tggtttacct
    64021 gaaccaaaac tagctctaat agccgtggct aaacctccta cacctacttc cacatcaaat
    64081 ccttcacttc ttagaaagtt tgctgtattt tcacatgctt taaattcttt aaatgctttt
    64141 tctggatttt cccatatttt cttacttaaa tcatcaagtt tgaatctttt tttatctatc
    64201 tcatttatag atatacttcc tatcattttc tattcctcct ataaatctat ttactatcaa
    64261 ccaaatataa ttggacctat tatactagca aatgccactg atgcaatggt tacagtaaca
    64321 aatcctccta taatcatctt tggtaataaa taatctgttg ccctttgttt ttcttcttca
    64381 gttccttcca tagcatcaat accttcattg gaaattatct gtgttgcagg ataagctagc
    64441 atacaagtta caccaattgc agctgacatt cttggagaat atcccaatag ttttcctaca
    64501 attgcagtaa atagtattat aaatacagag cctacaacaa gtattcctat agttggaata
    64561 atcatcccaa acaatgctgt tggagttacc ttagcaagtc ctccaggtaa catcaacata
    64621 caaccaaaca ttataattcc attagattga gatttatcta aaattccttt ttctaaaaaa
    64681 cctattcgtg taaaaatcaa ccctactaac aaacatgcta tgttgggatt taaataatag
    64741 tttgcagggg taaatcctgg tattaaagta gccaatccta caaaattacc aatcatagca
    64801 acaaatgaaa gctttgcaag atatactaaa gtagatgatt ttttacctaa atcttcttta
    64861 aaaattctca tacttggaag tttaaaatta ctttttgact catctttcaa aaaatccccg
    64921 ttatctcttt taaattttaa ttctttattt atacaatatg tagttatcgg aatgccaaag
    64981 aacttttgaa atgataatac tagaattaca aatgcagcaa gttcaggtct atttgcagct
    65041 tctgcaacag ttgttacgat aataccagca acagtagaac ccgctattgg aggtgctgct
    65101 attaaagcat actctctacc aaataacata ctacccactg ttaaacagcc aacccctatt
    65161 cctacaatac ctactaatgc aattacaacc gttttccatt catgacataa gtcttcaaga
    65221 tttataagtg ttcctaagtt agttattaat aaagctatac caaagttgct cattgcagct
    65281 aatatccctg atgctcctgt aatgtctttt ggtaaaatat ttgtccaaaa tcctagtaaa
    65341 aatataatac atacaaataa caatgttgaa attgcacctt ttgttttctt agataaaaac
    65401 tcactaaaag cataaatcat tacacataca gtaaagcaag taagtggatt ccaaaaattt
    65461 tccattgttg tttcctccta aatataataa ttttttatca ataaaacaat aattataaaa
    65521 tgtatattat gcagttattc ttaactacat atggtatagc ttgatatttt ttgatgatat
    65581 tatatacgtc ctaaattatt cctaaaatat ctaaataaat ataactagag ttaactctct
    65641 aaatattaac tatagttatc aacttactag atttcttttt gatttttact ttctacataa
    65701 ttttacgagt tagaattgta cttcaactat tctctatatt agtatacatg ttggaagact
    65761 tatatttatt taaatattag atatagaata tatttttata aacgtcttaa attattctta
    65821 aaatatctaa aacgaaataa taatataccc attaaaccaa tattatcaga atatacttca
    65881 ctttaagtct tctttatata gaaattttat ctattagtgc atcccctccc ttgtaaatta
    65941 gactatttct aagcgaattt agatatctta ttcctagaga ctattactaa aaacaaaaat
    66001 tatttattat attaaatcaa tgataagcca tactttaaaa ttctttctat acaagtataa
    66061 tttatcatat aatgacatct atacctttat ctttatagta g
//
LOCUS       DBGJFU010000006        60290 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.006, whole genome shotgun sequence.
ACCESSION   DBGJFU010000006 DBGJFU010000000
VERSION     DBGJFU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.006"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            257..1021
                     /locus_tag="ACOIAS_000380"
     CDS             257..1021
                     /locus_tag="ACOIAS_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGZ2700111.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(1302..2417)
                     /locus_tag="ACOIAS_000381"
     CDS             complement(1302..2417)
                     /locus_tag="ACOIAS_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGZ2700112.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(2458..3984)
                     /gene="glpK"
                     /locus_tag="ACOIAS_000382"
     CDS             complement(2458..3984)
                     /gene="glpK"
                     /locus_tag="ACOIAS_000382"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGZ2700113.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            4277..5374
                     /locus_tag="ACOIAS_000383"
     CDS             4277..5374
                     /locus_tag="ACOIAS_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HGZ2700114.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(5827..6339)
                     /locus_tag="ACOIAS_000384"
     CDS             complement(5827..6339)
                     /locus_tag="ACOIAS_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700115.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KMDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            6892..7773
                     /locus_tag="ACOIAS_000385"
     CDS             6892..7773
                     /locus_tag="ACOIAS_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HGZ2700116.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSIKDVEKFINFFSAIPAKDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(8086..8865)
                     /gene="hisJ"
                     /locus_tag="ACOIAS_000386"
     CDS             complement(8086..8865)
                     /gene="hisJ"
                     /locus_tag="ACOIAS_000386"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HGZ2700117.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLILGSDSHCAEEINKYDIV"
     gene            complement(9411..9761)
                     /locus_tag="ACOIAS_000387"
     CDS             complement(9411..9761)
                     /locus_tag="ACOIAS_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HGZ2700118.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(9777..10193)
                     /locus_tag="ACOIAS_000388"
     CDS             complement(9777..10193)
                     /locus_tag="ACOIAS_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HGZ2700119.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(10212..10403)
                     /locus_tag="ACOIAS_000389"
     CDS             complement(10212..10403)
                     /locus_tag="ACOIAS_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HGZ2700120.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(10554..11369)
                     /locus_tag="ACOIAS_000390"
     CDS             complement(10554..11369)
                     /locus_tag="ACOIAS_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HGZ2700121.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(11416..12354)
                     /locus_tag="ACOIAS_000391"
     CDS             complement(11416..12354)
                     /locus_tag="ACOIAS_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2700122.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(12351..13223)
                     /locus_tag="ACOIAS_000392"
     CDS             complement(12351..13223)
                     /locus_tag="ACOIAS_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2700123.1"
                     /translation="MNKNLSEKMEIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(13248..15176)
                     /gene="acsV"
                     /locus_tag="ACOIAS_000393"
     CDS             complement(13248..15176)
                     /gene="acsV"
                     /locus_tag="ACOIAS_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid activation/regeneration protein AcsV"
                     /protein_id="HGZ2700124.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(15288..15665)
                     /gene="gcvH"
                     /locus_tag="ACOIAS_000394"
     CDS             complement(15288..15665)
                     /gene="gcvH"
                     /locus_tag="ACOIAS_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGZ2700125.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(15752..17878)
                     /gene="acsB"
                     /locus_tag="ACOIAS_000395"
     CDS             complement(15752..17878)
                     /gene="acsB"
                     /locus_tag="ACOIAS_000395"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HGZ2700126.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(17935..18741)
                     /gene="acsE"
                     /locus_tag="ACOIAS_000396"
     CDS             complement(17935..18741)
                     /gene="acsE"
                     /locus_tag="ACOIAS_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase/acetyl-CoA
                     synthase methytransferase subunit"
                     /protein_id="HGZ2700127.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(18791..20158)
                     /gene="acsC"
                     /locus_tag="ACOIAS_000397"
     CDS             complement(18791..20158)
                     /gene="acsC"
                     /locus_tag="ACOIAS_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HGZ2700128.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(20189..21133)
                     /gene="acsD"
                     /locus_tag="ACOIAS_000398"
     CDS             complement(20189..21133)
                     /gene="acsD"
                     /locus_tag="ACOIAS_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HGZ2700129.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(21153..21923)
                     /locus_tag="ACOIAS_000399"
     CDS             complement(21153..21923)
                     /locus_tag="ACOIAS_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HGZ2700130.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(21979..23364)
                     /gene="lpdA"
                     /locus_tag="ACOIAS_000400"
     CDS             complement(21979..23364)
                     /gene="lpdA"
                     /locus_tag="ACOIAS_000400"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGZ2700131.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(23408..24289)
                     /locus_tag="ACOIAS_000401"
     CDS             complement(23408..24289)
                     /locus_tag="ACOIAS_000401"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGZ2700132.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(24325..24999)
                     /locus_tag="ACOIAS_000402"
     CDS             complement(24325..24999)
                     /locus_tag="ACOIAS_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HGZ2700133.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(25036..25908)
                     /locus_tag="ACOIAS_000403"
     CDS             complement(25036..25908)
                     /locus_tag="ACOIAS_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HGZ2700134.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(26000..26632)
                     /locus_tag="ACOIAS_000404"
     CDS             complement(26000..26632)
                     /locus_tag="ACOIAS_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HGZ2700135.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(26737..28413)
                     /locus_tag="ACOIAS_000405"
     CDS             complement(26737..28413)
                     /locus_tag="ACOIAS_000405"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGZ2700136.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(28662..29441)
                     /locus_tag="ACOIAS_000406"
     CDS             complement(28662..29441)
                     /locus_tag="ACOIAS_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2700137.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(29751..31670)
                     /gene="cooS"
                     /locus_tag="ACOIAS_000407"
     CDS             complement(29751..31670)
                     /gene="cooS"
                     /locus_tag="ACOIAS_000407"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HGZ2700138.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(32277..33188)
                     /locus_tag="ACOIAS_000408"
     CDS             complement(32277..33188)
                     /locus_tag="ACOIAS_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HGZ2700139.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(33304..33882)
                     /locus_tag="ACOIAS_000409"
     CDS             complement(33304..33882)
                     /locus_tag="ACOIAS_000409"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HGZ2700140.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
     gene            34108..35031
                     /locus_tag="ACOIAS_000410"
     CDS             34108..35031
                     /locus_tag="ACOIAS_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2700141.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(35203..36345)
                     /locus_tag="ACOIAS_000411"
     CDS             complement(35203..36345)
                     /locus_tag="ACOIAS_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGZ2700142.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(36363..38117)
                     /locus_tag="ACOIAS_000412"
     CDS             complement(36363..38117)
                     /locus_tag="ACOIAS_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HGZ2700143.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            38253..38459
                     /locus_tag="ACOIAS_000413"
     CDS             38253..38459
                     /locus_tag="ACOIAS_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700144.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(38464..39387)
                     /locus_tag="ACOIAS_000414"
     CDS             complement(38464..39387)
                     /locus_tag="ACOIAS_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700145.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(39641..41341)
                     /gene="argS"
                     /locus_tag="ACOIAS_000415"
     CDS             complement(39641..41341)
                     /gene="argS"
                     /locus_tag="ACOIAS_000415"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGZ2700146.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(41751..42164)
                     /locus_tag="ACOIAS_000416"
     CDS             complement(41751..42164)
                     /locus_tag="ACOIAS_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HGZ2700147.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(42230..44608)
                     /locus_tag="ACOIAS_000417"
     CDS             complement(42230..44608)
                     /locus_tag="ACOIAS_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGZ2700148.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            44927..46378
                     /locus_tag="ACOIAS_000418"
     CDS             44927..46378
                     /locus_tag="ACOIAS_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HGZ2700149.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(46582..49095)
                     /locus_tag="ACOIAS_000419"
     CDS             complement(46582..49095)
                     /locus_tag="ACOIAS_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2700150.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTSQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKALDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(49318..50139)
                     /locus_tag="ACOIAS_000420"
     CDS             complement(49318..50139)
                     /locus_tag="ACOIAS_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2700151.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(50657..51133)
                     /locus_tag="ACOIAS_000421"
     CDS             complement(50657..51133)
                     /locus_tag="ACOIAS_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGZ2700152.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(51168..51746)
                     /locus_tag="ACOIAS_000422"
     CDS             complement(51168..51746)
                     /locus_tag="ACOIAS_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGZ2700153.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGNEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(51850..54450)
                     /locus_tag="ACOIAS_000423"
     CDS             complement(51850..54450)
                     /locus_tag="ACOIAS_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGZ2700154.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNVFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
     gene            complement(55020..55703)
                     /locus_tag="ACOIAS_000424"
     CDS             complement(55020..55703)
                     /locus_tag="ACOIAS_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HGZ2700155.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAVIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(55879..56469)
                     /gene="zapA"
                     /locus_tag="ACOIAS_000425"
     CDS             complement(55879..56469)
                     /gene="zapA"
                     /locus_tag="ACOIAS_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGZ2700156.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(56494..58887)
                     /gene="pheT"
                     /locus_tag="ACOIAS_000426"
     CDS             complement(56494..58887)
                     /gene="pheT"
                     /locus_tag="ACOIAS_000426"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGZ2700157.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(58904..59923)
                     /gene="pheS"
                     /locus_tag="ACOIAS_000427"
     CDS             complement(58904..59923)
                     /gene="pheS"
                     /locus_tag="ACOIAS_000427"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGZ2700158.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(59973..60207)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        18649 a        11397 c         6775 g        23469 t
ORIGIN      
        1 atctctatac tagtaaatta tataataaaa tcatatttta gtcaattgtt ttataaaatt
       61 tgagttattt tgaattatta aacatctatt tttacttttt tgcttaaatg taagttttaa
      121 atatggtaaa attatcttgc ataacactta taatattatt tgataaatat acatacatta
      181 acaaatatac ctaaactctg acttaattgt aagttatgcc aaaactaaaa taatactagt
      241 ccaaggagga tattttatgt taccacagga aagatatgag aagattttaa gtattctcaa
      301 atcagataat ataattaaaa taaatgacat agttcaaatg tttgatatat caatagaaac
      361 tgcacgaaga gatttagccc atatggagag tatcggtctt ttaaaaagag tttatggtgg
      421 tgctatacca gttatagcaa gtagcgtaga acttaactat gttaaaagaa aaaaaataaa
      481 ctcaaacgat aaaaaacaga ttgctattaa atgtagtgag tttataaata caaatgatac
      541 tatttttatg gatactggaa ctactgtact tgaagtttca aaaaatttga aagataaaaa
      601 gaatctaact gtaataacca actcaatttt agttgcaaac gagcttatga atactaatat
      661 aaaagtatat gtgcttgggg gctatcttag agaaactgaa ggttcttttt ctggtccgct
      721 tacactgtca gctattgaac aatttaatgt tgataaagct attttaggcg ctggaggtat
      781 cactcttgaa aatggaatct ctgactatca cttagaagaa gcacttgtta gaaaaaaaat
      841 gattgaaagg tctagaaaat ctatagtagt tgtcgaacct cctaagtttg gtataaattc
      901 atttgcatca gtagctccta tagaacatat agatactatt attacctctg ctagtataga
      961 ccataatata ataatagagt tttctcaaaa aggaatacct ttagtcatag ctgataatta
     1021 gcttatatga ttgattttta gttccttgat gtaataaatt aaaattaagg agccaaataa
     1081 ttatttattt agctccattt atgtctaaac tatactttat attttaatta tgtaactact
     1141 ttcagttagc ttatcatttt tagttatttg attgccattt tatttaattt ataagtttta
     1201 gttattcgat tattttttaa ttgtctgtct ttttaattta tttttagtta tttaaactcc
     1261 tactttaaaa taatttaaat ttgacttaag tattgtttat tctacactgc atctttcttc
     1321 attttaatac tttgttcttc ttccaaggtt aaatcttttg tcataagtat agcaagtgca
     1381 attgccaata ctcctccaac caattttcca attacaacag gtgtaatcat ctctggaact
     1441 actcctgctg taaatcctaa atggtccccc aaagctgctg tagcacaaac cagccaagca
     1501 gtatttacta ctttcccctt tggactcata tctttcatca ttttatatac tggaatacta
     1561 ttggctaaag taaaaactaa tcctgctgca ctagtggcat ccatacctaa ttttccaccc
     1621 actattgtaa gtggtttatt aagaacttta actaataaat gtaatattgg aaaagtacct
     1681 aataacacga ttgcaatact tccaattact tgaatacctt ctcttatagg tgtcatgcct
     1741 tttataagta ctatacctgt tatttcttga aatgctgctg ctcccaaacc tatcgttatc
     1801 aatatagata taaattttcc aaaaactaat gacccattta tcataagcgt agaattaaat
     1861 tttaaaccta ctgccaatat tactgccaat attatcactg gtatcatatt tataattacc
     1921 attgataagt taaatcctgc tattaatcct cctataatac ctccaaaagg tattgttatc
     1981 aagcctatca gtaacccttt tgcaaagaat ggtctgtctt catattctat aagacctagt
     2041 cctactggaa ttgaaaatac taatgtacat ccaagcatgg atgacacaac caatcctgca
     2101 agcattcctg cctctttatt gtgtgcaagt tccatagcca atggataccc tcccatatca
     2161 ttcgctaaaa tagttgcaaa catagatggg tcagctccac agagattgaa gattggtatt
     2221 attataggac ctagtacatc tgaaattact ggagctaaac aaactatacc aaccattcct
     2281 aaagccaatg gacccattga attaaaccct tcttcaaact gctcgccaag tccaaattta
     2341 tttccaatta ttctatctaa cgctcctaaa agcactccca cagccattat gtataatata
     2401 attttatcaa agggcatttt taattcctcc tatacatgta aaatactcta ttgtaactta
     2461 attagcacac acctttttgg tatcgttttc ccaattcata gctcttttta ctgcttggtt
     2521 ccatccaagt aatatttctt ctcttttttg agtagtcata tttggaatat attttttgct
     2581 tattgtaaat tcacttctaa tttcatcaat actgttccag acaccacaag aaagacctgc
     2641 catatatcct actccaagta ctgtcgtctc aggattttct gcaatttcaa ttggtatacc
     2701 taatatgtcg gattgaaatt gcattgtaaa gttattttta gtaattccac catctactct
     2761 caacattttt atttttttcc ctgaggctaa gttcatagaa tcaacaacat ctttgacttg
     2821 aagtgcaaca gactcaagag tcgctcttac tatatgctct tttttagtac ttcctgttat
     2881 tccaaccatt gtacctctag catattggtc ccaataaggt gctgctatac ctgcaaatgc
     2941 tggtacaaaa tatacacctc cagtacttga tatggaattt gccatgtcat cagtttctga
     3001 ataatttttt ataactccaa tgccatctct aagccattgt attgcagcac ctgctatata
     3061 cactccacca tcataagcat atgtaacttt atctcctatt ttccaagcaa cagttgtcaa
     3121 aataccatta cctgctatat ttagttcctc acctatattc atatatagga aacatcctgt
     3181 accataagta tttttaacca ttcctggttc aaaacatcca ttgccaaata aagctgctgg
     3241 ttggtctacc attgaagcag taatttttat agaaacccca caaaatgcct ccttatctgt
     3301 agttccaaaa tcaccactcg taggcattat atctgctaaa atacttctag gtactcctat
     3361 aatgtcaagc aactctctat cccaatccat atctcttata ttaaaaagca ttgttcttgc
     3421 tgctgtagat acatcagtta aaaaagattt tcctcctgtc attttccata ttaaccaact
     3481 atctaatgtt cctgcaagta cttctccatt tttaattttt tcttttacac cttttacatt
     3541 atctattacc catttaattt ttgtacctga gaaatatggg tctataatta acccagtctt
     3601 atctattatc ttattttcta aacctggtat attctttaaa ttttccacat aatcataagt
     3661 tctcctgcac tgccacacaa tagcattgta aagtggttta cctgtatttt tatcccataa
     3721 cattactgtt tcaccttgat tatctatacc aattcctttt atatcacttt cattgattcc
     3781 attttcaaaa gcctgttgta atacattatt actcacagtt tttgtattat tccaaatttc
     3841 tatagggtca tgttcaacat atccagattg tggatatatt tgagtatgtt ccatataatc
     3901 gctaaaaaca aactctgatt tttcgtttat caatactgct cttgttcctg ttgttccttg
     3961 gtcaattgct aatacatatt tcactttaaa tgtccccctt tattttcaga attaataaat
     4021 tcttttttat tttttgaata tataatctat tcaagttatt tatattttat atcatcatgt
     4081 ggatttatgc aatattcatt acaatatttt ttgttatttt taattatagt gattaaaaaa
     4141 gtatttttat tgaaatatta ttgcaatatt tattatttta ttctataatc tagttaaaat
     4201 aacacataat attttaatat ttacacataa tcagtcattc aaaaatttaa atttaacttt
     4261 ttaaggaggc tttacaatga aaaaaacata tctattcagt cctggaccag taatggttac
     4321 agataaagtc agaaattcac ttcttcacta tgatatatgc catagaggaa atgagtttat
     4381 ggatttattc aaagatacac aacaaaaaat caataaacta tataatgcaa cttctaatta
     4441 ctactcatta attgtatctg gctctggaac atctgtaaat gaatgtgtac tatcttctat
     4501 ctttgacaaa gaagatgcag ttctgctagt tagtaatggt gtttttggag aaaggctaga
     4561 agaaatatta tctacttata atgtaaaaac atataaacct aattttgaat gggcagaata
     4621 cccggaccta gacattttag aaaaatacat aattgaaaat tctgatataa aagtaattgc
     4681 tatggtgttc catgaaacta gttcaggaat gataaatcca gtccctgaac taggaaaact
     4741 tgctaagaag tataacaaaa tattttttgt agatgcaata agtgcctcag caggtgaata
     4801 tattgatgtg gaagagttta atatagatat aataacaggt gttggtggca aagctcttgg
     4861 agcatttcca ggttctgcat acctttgtgc taaagagagt attttacaaa atattaaaga
     4921 aaatcaatgt aagaatgtgt atcttaatct ttataaacac tataaagttg ctaaatcatc
     4981 ttctcaaaca ccaaatacac ctaatgttac tttaatattt gcccttaatg aagctttaac
     5041 tgagtttttg gaagatgaca gtaaaataga aagatacaaa gaatgttctg ctattttaag
     5101 gaatggtatg gaagaattgg gtctaacatt tttacttcca gataagtata tgtctaatac
     5161 agtaacatct gtatttctac ctaaagaaat caatgtaaat gagtttatat tagggctaga
     5221 aagggaaagt ggctatgttg tctatccagg aaaaggtaaa ttcctagatg ctaatatatt
     5281 ccaagtagca aatatgggag aaatatatcc agaagattgt tataaattct tagatatctt
     5341 aaaatcaaaa ttggagataa aaaaagttat ataacagttt gtttgtaaaa tcctacaata
     5401 tatcagtata aattaaatag actgattttt ttaaattttc ctataataaa ttatatttta
     5461 tatttttgta taagggtgaa ctataatatg taaagttcac ccctatttca aatctttttc
     5521 cttcaatata atctttttct tttaatttat tttatatttt tgacataatt attcttacat
     5581 ttaattgttt tttatttttg acgcaatcgt tttttattca gtcattttat attcttgata
     5641 caatcattct tacacttaat tattttttat ttttgacaca atcgtttttt attcagtcat
     5701 ttaatatttt caacacaatc attcttacat tctattattg tttgttttga tacaactgtt
     5761 tttattcagt cattttatat tctcaacaca attacctttt cattcaatca aagtaatctt
     5821 taagcattat ttttcaagct catcaaagta attatatacg ataacctcac ctgtctcttt
     5881 atctgtagtc tttataatgc ccttagctcc atttacctct tcttttgtaa attcatcatc
     5941 taacacaact ttgaatttat cttcctttcc ataaccttgt tgaaattcat ctctcaaaat
     6001 tgcatttttt tctgcttttg gcttcaactc aaattcaatt ccagatttca tttcatcaaa
     6061 ataatttaat actacaacct cattattcat tttattagtt gtctttataa tccctttagc
     6121 tccatttact tcttcttttg taaattcatc atccaatgtt atattaaatt tgtcttcttt
     6181 tccatattcc tcttgaaaat ccatcttcat ttcattgtat ttagtttcaa actctttact
     6241 cttgtttaat tccatagctg atacatttgt caatgatgct ccaattgcca ttactgttaa
     6301 tgccattact ataccacttt taaatttttt tactttcata tcttacctcc taaattaaat
     6361 gttctcaatg aaattatata aagaaaatcc taagttttac agtttcattc ttgtaactaa
     6421 aattactatt atgtaaccaa actttttgct tcaactattc cttaaaagtt gcttttttga
     6481 ggaatagttg aagttaaata attagatttt atctatattt atagatttaa aatcaaattt
     6541 atccatataa ataaatactt atttttgcaa aataaaaatt tttaattact ccaatccttc
     6601 ttatttactt aaaatcctat gataagaaat ttaaaagtct ttaccttctt taacaataat
     6661 ttatttactt ttaacataca aaatatagag agtatatcaa aatcatttta ctgaatattc
     6721 taaaaataag taccccctat tagtatatat atttacatat taaaaaaaag ttttaaataa
     6781 agttatattt tttacaacag ttttgtaatt aaaaacatat tgaaggtaaa tttaaaggta
     6841 ctatatacta taaatatgtc gacacaataa atttaaaaag gagaattaaa tatgaaaaaa
     6901 agattaataa taatgatatt gtctgttgtc ttagtactat caagtatact tacgatattt
     6961 gcatattcaa atattaaata taataataat aaattgatat attctaatat gatagataaa
     7021 aaaactcaaa atagtgtaaa ggaaatccta aaagaaaata aaattaatga aaaagacata
     7081 gatacattta ttaaagcagt caacaattat aataaattgc aagttaaaat acttcaaaat
     7141 aatataaata tatctaaatc tggatattct tcaatcaatg caaaacaagt tccatataac
     7201 ctagaaaaat tgcaagataa ttgggtgaaa aaattccctg attatatgga tgtaaattgt
     7261 agaataactg cttttagact ttttaaagat tttataaatt caaataagaa atttactgga
     7321 gactctattg atttgaatgt cgacttggac actataatga ataataaaga tgctaaattt
     7381 agcatcaaag atgtagaaaa atttatcaac ttcttttctg caataccagc taaagataca
     7441 gatgatacaa tcaaaatttc agaacaaata aaaaatgaat ggaaaaaaag aaaaatatct
     7501 tttaaaaata ataaaaatat atctattata aatggatttc ttcgttatcc agaaactaaa
     7561 aacgtattta taggtcatac aggcatatgt attaaaacta aaaatggcat tctattttta
     7621 gaaaaatacg gtgttacatc accttatcaa gttactaaat ttaaaaataa agaagatgta
     7681 aaaaattata tgtttaatag attgaagatg tcagaaggtg aaattgaatt acctgaccct
     7741 ataataatgg aaaacgataa acttatgaaa taataacttt aaacgcaaaa taatatttat
     7801 aattactaag tcaatataat agagaaggtc tatagatttg tgtaaataca agcttatcta
     7861 caatcttata taagaatatt tatataaata agatagatta attctaagtc ctattattct
     7921 attgaatttt attattattt tatttagatt ctattgttat tttacttaga acttattatt
     7981 attttgttta agttctatta ctattttcaa tcatataaat ttacaaaaga ctgtacataa
     8041 atttatttta tacaaactta tctacagtct tttgtgtgaa tatatctaaa caatatcgta
     8101 tttatttatt tcctctgcac agtgagaatc agaacctaaa attaatggaa tatcgtattt
     8161 ttttaccaac tctaataagt atccatctat ataaggctct ttgcagtact cttttctatt
     8221 tccagaaaca ttataatcaa gttcataacc tttttcttta attaacttta ttaactcttc
     8281 aagaattgta tttcctttat aatcgtatgg aaatacctga ttaaactttc taactaaatt
     8341 taaatgacct attctttttg gtttatgtat acctaaatct gaatttacag cttttatcaa
     8401 tgtttcataa tacttattat aaacagcttc aatacttcct aatttgtcga ttaaattttg
     8461 aaactcctct acactgtaat ctacacaata ataatcatca tcaatcttaa ttatatgtac
     8521 tgataaaaga gaatcctcta attcctttcc atatttattt aaattctcct ttatcccttc
     8581 ttcgtatcct tctataaaat ccacttccga cccaacattt atcttgattt taccctcata
     8641 cttttctttc aacttcttga catcattaaa atactttggt aaatcttcaa tactcataca
     8701 actatcattt tcaggagcag ggtctctaaa tccatttgca tacggaaaat gttctgtaaa
     8761 tgtcatttct gttattccaa catccattgc tctttgtaca tatttttcaa aatcatcctt
     8821 acttccatga ggacaataag gtgaatgtat atgaccatct ttcataaata attcactcct
     8881 tttttaaaga aaaaatctaa tataaatgca taaatctttt aacttcaaaa taatattttt
     8941 aatcatgcta atttttatta tatctaatat tagagtaatt ttaaactgcc ttttaagtat
     9001 cttatcatta gtactatttt caacttaaca ttaatattat tttttaactt aacgttaata
     9061 ctatctttaa atagtaatac tcaatatata attacttaac ataaaataac tcaaattgct
     9121 tgatgaggct tttgcagtat ttaaagaata tataattatt taacattaaa taatccaatc
     9181 ttgtaataaa atttactcat taaagtatat tacagaaaat acctcaaaat aaacttttta
     9241 gttcatgata tactcttttt tgcatgaaat tagatatatt tttatcattt caacaatgaa
     9301 agggaggctt atatccatct ttagacaacc ccttagataa aatttttaat aattaaacat
     9361 cacaaaatat aaaaatcgct tatatcaaac gtataagcga ttaattatat ttacatatgt
     9421 tttttagcct ctaacaacat tttattagct tcttccatat actctatagc cttttcaata
     9481 ctctttgctg tttcttcttt tccaatagct ctagcctttt caacccactc tctaaatcct
     9541 tcttcatgag tttcattgtg atttatccaa tgtactaaaa gtatttttaa agttttttca
     9601 tccttagact ctgatgattc ctcatgacta tgttcgtggt cgtggtcgtg agcatggtca
     9661 tgtggatgtt catgacatat atctccatga gtatgttcgt ggcagtggtc atggtcatga
     9721 tgtccatgat gatatcttac aatctcttta tcaaaaatca ttagttattc cccctgttat
     9781 tttatattaa actctttttt tataaggttt acagaatctt ttacaagtag tgctaatggt
     9841 ttaccttcta atccaactaa ctccacattt tctggcataa taggtaaaag tatcttaagt
     9901 gcctcactgt ctgcaatagc ttcacttata ttacaagtaa cttctcccat catagaatta
     9961 ggtattatta cagaaatagg tgcaactatt atatttgctt ttttagctga tactactata
    10021 gcattttccc ctgtagctcc tttattagca tgagctttca tcatagaact agttgctata
    10081 gaattagtcc ctagtgcata aatttctaca tatgtaggta actcttctct caaagaagaa
    10141 actatctggg ctccaatacc tccacccatt ccatctatca ctgctattat catatgaaat
    10201 tcccctatta attaattttc ttgtattatt atcttatggt ccatcaatct tatttcttta
    10261 attatcccat ctattatctt ttgctctcct aataaatccg ttaaataaat ctttccatca
    10321 caagggtcta tattaaccac attttccata accttttcta actcattatt acttttacaa
    10381 atgaaagctg atgattcaca catataaatc tcctcctaat atgattttta ttaaaaatca
    10441 aaaaaagatt cccttaggta tttcaataca ccaaagaaac cttcatgatt tcagtttttg
    10501 tatattatta taccttaagt atatcacttt aatttatgta aagtcaatta aagttaacgc
    10561 tttttattat actcttcatt cttgtatttt tcatatagtt ctttaactaa ttgcttctca
    10621 taatcagtca aatcgccctc cacaaactca acattaaatt gttcagctaa tccctttact
    10681 atgtttttct gtagaatatc tatgtctatt tttctgccaa tttcattttc tattccactt
    10741 gcttttttag ctgtaaattt catagctctt tctttcaatt ctggtgtctt agtttttatt
    10801 aatttaaaca atttttctac atcaaaatcc agtattattg aaccatgttg taataaaaca
    10861 ccatcttttc tgctttgagc acttccaatt accttcttat tattaatagt aacttcatat
    10921 gatgcatgtg cattaaaaca tgcagctgat aaattttccc tacttattct ctcgcctcta
    10981 tttaaattat ctgtttctat accacttaga ttcaaccctt taactagacc ttcacttata
    11041 tatctataag ataggttgat gctcttatcg attaaaggat tatcttctcc tataattata
    11101 ctataagtta gctcattatc atgtaataca gctcttccac ctgtaattct tctagtatag
    11161 tctatattca tacatctaca cttatctaaa tctatctcat cttccaattt ctgaaaatat
    11221 cctatactca aacaagctgg ttcccatgta taaaatctaa gagttggctt attatgtccc
    11281 ttcttatatg cagtaaatat agcttcgtca attgccatat tcattgctcc ttcatatgat
    11341 ttattatgta taactctcca ctgattcatg attattacgc ccccattttt agtataaata
    11401 tatcctagaa atcctctata gatttatttc tcttattgct aaatctatct cactttgatt
    11461 taaaggtctt gaatagttgt atatagtact tcctggtctt tcattatagt atggtctatt
    11521 acaatcctta caacctctta tttcaaaagg atagcctttg tttatgtctt ttaaaatatc
    11581 attgtcaagc tttatactat ttaagtatcc atttttaaac ttaaaatact cttttggata
    11641 acctttattt atcatataag acattaactg tacccttcta taactttcta tgcttggttg
    11701 atttatcttt tccattttag ttcctctaac tggagtaaat gcaaataaac taatcgttac
    11761 atcattttcc tttaaataag tatataaatt gtatatatct tcatgacttt cacccatacc
    11821 aactattata tgtgtactta ttttattggg atatgatttt gacatttcag ttataaattt
    11881 taacttttca tcatagttat ttccttttat tttttcatat agttctttat ttgctgcatc
    11941 tattgctatt cctattttat caactcctac tgaaaaaaac ttctttatct gttcatcatt
    12001 ttctaatttt gcagatagtg aaattggcat gtctatctta ccacttatat agtttataaa
    12061 gtctaaaaca gagttatgtg cttcttcact tgccattgat tgaatacata ttctctttat
    12121 attttttcct ttataagctt ttaaagcatc taatatttct tcttttgaaa actctggcca
    12181 aattactctt gataatttat cttttctagt actactttct attgattgag aacaaaaact
    12241 gcatttattt atgcattttt ctccaatcat tatatatgct gtggtaggag gtatatcgct
    12301 ttttttgttt aatatcccaa gttctattgc tgtcccaact gatagtctaa tcataaaacg
    12361 caacactcct tacactcatt tattgtcata ttcatttcta tagccttatt ctttgcagac
    12421 cttgatggta atactattct attgactcca cacattatag ataattggtc tagctctttt
    12481 ctatacactc ctcttggtct catacaacct agattaattt cagtatctgg taaattaatt
    12541 ctagcttcac ataaaatatc tgctacccct tctaactcag gaggttctac attttcatac
    12601 tcagtccctt ttgttggaat aaggactata aatgtaagct tattaggagg atttttttgt
    12661 aggtattcta taatttcata ttcacccttt atttgtcctc cttttaaacc aatacatata
    12721 tgtggtgcta cttctgtatg ttcttgtatt gtattataga cttcaatata atcttcttta
    12781 ctctttttca tcttataaac ttctcttatt gtctcatcat caaacactaa atcaaaagaa
    12841 actatatcta aatacttaca caactctaca atgctatctt tatccataag acctaaatga
    12901 gcattcaatc tatatccttg ctcttttaag tttttaatgg tattaatatg agtatttata
    12961 gggacatctc cctcataact acaaccgcca ctcaatagaa agctagttat attccttgat
    13021 tgtacttttt cctcatattc ttctattggc accatattgt ttaagtagtg accgttgcaa
    13081 tgggcacatt tcaaactaca ttgattgctt gtagtactaa gagctagtgt ctggttagga
    13141 taagcaaact ctattttatt tccaaaattc tttaatttaa tatcaaatgc tatttccatt
    13201 ttttcagata gattcttatt catagtttat tccttcttaa gtacatttta ctcttctagt
    13261 aatttctttg ctgttgggaa ctgctctata ttcgtatgtg gtaagaaaca tgcagaaata
    13321 aattcatcca tataacttgg atatacacta agttcaacat aagttatttg attacctatt
    13381 tcttcaagtt tattttttgc atctttactt ataagagcta agtaagaacc aacaagtgaa
    13441 ctattcccta tataagtaaa tttttctcta tcaatatcag gaagtaaccc tataattatt
    13501 gagttttcta tgtctaggtt gtttcctata ccacctgcta tataaaccct atctaatata
    13561 ctaaaatcca tacctaaact atctacaaga gtgtatgcgc ctgaatatat agctccttta
    13621 gctctgatga aattatctat atcaacttca ttgactacta tatcattttc taaatcaaat
    13681 tcttctttaa atgctaatac atactctcct atctcatgct cattaaatct aactctctta
    13741 ttatctaaat ccctgtatat tttacctctt ctatctatta cccctttagt aatcatttga
    13801 cagattaaat caattatacc agaaccacat ataccaactg gtgcacattc atcaataatc
    13861 tttaatgttg gctctaaagt ttctttatca ataattactt tctctattgc tcctgctgat
    13921 gctctcattc cacagcttat ccctccacct tcaaaagcag gtcctgctga acatgcacag
    13981 ctcatcatat aatctttatt tccaaataca atctctccat tagttcctaa atctataaat
    14041 aaaacatttt cttcacttga ccatatccct gctgataata cacctgctgt tatatctcct
    14101 cctacataac ttgcaacaga tggagctaag tacacatatg cactgtcatt tacaaaaaga
    14161 ccaacatttt cacccataag ttttggagat tttaaaaatg gtggtatgta aggttcttgt
    14221 cttaagaagt ctgtatacac acccaagaat aaacttgtca tagttgtatt cccagcagcc
    14281 actaatgtaa ctacatcttc cttatctatt ccatttcttt catatattgt ttttaatagt
    14341 ggatttatag tttcctcaac tatagcttta tgaagtgtct ctaatccatt tttcttagtt
    14401 gaatatataa ttctatttat aacatctgct ccatatttta tctgtgcatt tccagaagat
    14461 gctttatcta ctacctcttt tgagtatagg tctactaaac aaacaactac tgaagttgta
    14521 ccaatatcta ttgcaactcc gtataaggaa ttttctttat ttccttgttc tatatttatg
    14581 attgtaagtt tcttttgttt ttgaacatat gttattgtaa ccttaaaatc actctttcta
    14641 aatacagttg gcatttttct aagtatatcc aatctaaaat ctatttcatt atatcctagg
    14701 ttatttctaa catatctttc caatctatct acatcactta tattatcatc taaatttggt
    14761 tcttccattt ctatgtattt cttttttata tttgttttaa attgaaggtt atgttcttca
    14821 attatttttt tagctctttc aaaaatttcc ctatcctctt ttttattact accttctatc
    14881 ttcataccat gcatagaaga tgatacttta gaaggaactt caatttctat atctgatatt
    14941 acctttgtac aacaagctag tatataacct tgctcccact catcatctgt tatatgtctt
    15001 gttttttctg tatcaacttt tccattcaat aattttactt tacacttccc acaagacacg
    15061 tttccattac aaggagcatc tatgaatata tctgcatttc ttgcaacctc tagtaatata
    15121 tccccctcat tacaataaac ctctttattg tttggtgtaa aacttacttt tatcatgtta
    15181 tccctcccaa tttgttctat atatgataaa aggaaagtat agctaaattt gcgtgcactc
    15241 aaattttact atactttccc caattataat aatatcttaa tctttaatta ttctaatcca
    15301 gcctcgtatt ctttatcact taataacgta tcaagttcag ccacatcagc tagttttact
    15361 tttaatatcc agttttcata tgggtcttca tttatacatt ctggctcatc ttctaatttt
    15421 tcattaactt ccaatacctc accattaaca ggagatatta aatcagaagc tactttagaa
    15481 gactcaacta ctccaaaatc tactccctta gttacagtat cttctacttc tggtgtttca
    15541 acaaacaata tttctcctaa ttgatcttga gcatagtctg ttatacctat atatacaaca
    15601 tctccatcaa ttacttttac ccactcatga tctttagagt attttaattc tggtaataat
    15661 ttcatttcga tagaccccca atattatatt attgattaaa ttacttaaat catattaata
    15721 ttatatcata ttattttaaa ttttccatca attacattac actttccata gctaatgctg
    15781 gatgaccttt ttcttctaag aaagctaata ctccttcaga atcgtcagct atagtttcat
    15841 cacaaaccat atcacagaag ttttctattc cagtcatttc tttaactgta gcatttaatt
    15901 tatcagctac ataatctttt aattcttttg gcatccaaac tattctttct ggtccacctt
    15961 ctgcataagc gaatttttta gaagatatga aatgtcttcc gtgtcccata aatcctggag
    16021 tttgaactcc tccacctgtc atagaagcaa gttctccgaa agtcatacca actggagtaa
    16081 cacttgcaaa ttctctgtta actacaacaa atccatttgc ttctggcatt ataccacaga
    16141 tacattcgaa gcatccacaa gaagtcattg ggtcttctag tatagagtat aatgtaacag
    16201 attcaactgc accttgagat atttggttta cagtttcatt tactgaattc catacacctg
    16261 ttctatcatc taagcactcg cctttttcta ttggttgaca aggacctgtt gggtctaatt
    16321 ctttagtagc cttagcatct aaccagctaa ctgctccaca aagaccaagt ctttcaggag
    16381 taacaacaca aacgtgagct ggtgcaaatg attgacataa gttacaagaa tagaaagtat
    16441 caacactttc atcaactaat gaagctaatc tttcatctct agcattgtac ttagctattg
    16501 cttctccttt taattcagaa actttttcag catctgtaat tatagttatt tcacacttat
    16561 caactactga ttcaaattca tctaacatct tagcatataa aacttctcct atatgctcaa
    16621 gtctaaatcc tttatcaaat gcatctttag agattcttac ccaagccata tctctttgtc
    16681 caacgtgcat tacaccttct atatagttta agaagtagtg gaaacgtctt tcaagaactg
    16741 gctcgaaatc ttcttgcata tttttaccag ctattttaac tattacagca agtggtaatc
    16801 ttaatacacc atctgcatca acttcatcta tatttggtcc tattatttct atcttatggt
    16861 cttcaacttc tgttgcttct ttcttaacaa caagctccca agcttcagtt ctgtttccac
    16921 caaactcagc aaacatatcg ctctttctaa ttctctcacc ttcaaatgct gctgcaaatg
    16981 aaactgggat aggtatttca gtaactttta tttttattcc tctagcttct aatgaagtag
    17041 ctactatctt atcgtaatct ttttgagtta ataggttcat tggaacttca agaactgttt
    17101 ggtcagttat aactgggaat cctaaagcta ttgctccagc acctgcagat acaactaatt
    17161 cacttaatgg tccaaatgca ttaacaaatg caggaactct attagctgtg tactctaata
    17221 atccgtttaa atcaccagga gttaatccac cgaatattaa tgctgctctt actgcaacag
    17281 atacaacgtg tataacagaa gttacatcat atcctagagg tataactctt agctcaagac
    17341 ccatttttac tccagcttct atagcttggt ctataacttt accaactaag aaagttagta
    17401 aacctttaga ttggtaatct tttataactt ttgctgctga ttctgcatct ggacactcac
    17461 caagaacaac tgcaacacca ggtatatctc ctgtaactaa tggtacacca agtgatctga
    17521 ttatagggtc agttatatga cctgcacatg gctcactata tggagcatcc atagttgaat
    17581 attttaatgc ttcaattact tctgctgcta aagctgtagc taaaccagca ttaaaagcat
    17641 gttctagtaa atgagttcta tttattaaag attttactac ttctaaagca ccttctaagt
    17701 cccctaaagt gttcatcttt tgtcctgttg cagcatatat acaaggtaat gaatatgctg
    17761 tatcagggaa agcaacttta tgctcttttc cattcttttc tatagcttca gctaacatag
    17821 cttgagccgc acctaaagct tgttctgacc ctgtaaagat tatattatat agattcattc
    17881 tatttccccc tttaatattt acacctattt tttattaact ataccctaaa aaccctagaa
    17941 agcaaatcca ccttcattta actctaagaa agagtctgca tataaagatg ccccgttgat
    18001 tatgtcacaa gattttacag ttttcattaa ttctgggtca catggattca ttatagctga
    18061 actaaatcca ttagccattg ccattgctaa gaatgcacta tctaaaacag gtctaacatg
    18121 cttaggacat ccattagata cgttagataa tcctcctgta gttaaaagtc ccatctcagt
    18181 catcattttt atagcttcta aaacttctac ttgtttttct tgcatacctt ttataactaa
    18241 gcataatggg tcaaatagta tatcagttgg ctccattcct aacattaagc ctttttctaa
    18301 tatttcttgg cagtaagcca tacgctcatc attatctctt gggatacctt cttttgcaca
    18361 taaacctata accattgctt cgtattcagc tgctatatct attaaatcaa atcttgaacc
    18421 agcatcagca gagtttatta ttggttttgc attagttcta tcataaactt taagtccagc
    18481 ttcgatagct tttttattag ctgtatctaa tgctatagga acattgttga actcagattg
    18541 aagtaatttg attccccatg tcattatttc ttcgccatct ctttcggcag gtcctatatt
    18601 aaaatctata tagtgtgctc ctgcttctaa ttgctctttt gctcttttta agattggagc
    18661 tgggtctctt tctgctaatg cttttcttat tgaaggtgat atacaatgta ttctctcacc
    18721 tataatcata aatttttcca tttataatgt ccccctttaa atttaatatt aatagttagt
    18781 taatatttta ttagtttgtt tggcatgctt tttcttggta tccttttaag aacttaggta
    18841 attccataga ttcctctggt cctataacta cattccatcc tggtaagtta tcttctatat
    18901 cacctttaag tactgcaact tttcctggaa tgataaggtc tttacaatta gttacttctt
    18961 ctaccttact ttctttaatg aaagcagaga ttgagtttcc accaaattta ccagcagccc
    19021 aagatgtaag aactgaatat ccacctgcat ctggtattac taaccatact ggaacttttg
    19081 atctttctat atcaccagca actatgaagt aagttaatgc aaagtcaaca gtaactaata
    19141 ctggagaatt ttcatctggg ttgttgattg gataaatctt tggttcaact ctcattggtc
    19201 tttgtgggtc agtgtatatg ttttgtctta gtgcaaataa tggtaatgcc ttagcataac
    19261 taatatcatc tattactatt atagaaccgt attttatagt gaatattgat gataaagcaa
    19321 cttccatcat agggtttgaa ttagataatc tatttgcaaa tactattgaa ggatatccaa
    19381 atgttctatc ttgctctttt aaagctgttc ttctaacttg tatagcatta gtgtaagtat
    19441 cttttatgtt ttctcctgtt acatctaata ctaattcttt atatccagca gcttgaatta
    19501 attcaactgt ttcatataat tcttctaagc taccagcttt tacacctaat ggtaaacttg
    19561 ctcctttaac tacttctatc atatctttgt agttctcttt agttgctcca tataccattg
    19621 gtctagcatc ttttaatact tcaacagctt ctttaactac ttgtgcatca tcacaagcta
    19681 aaatataagc tacttctaat ccactgcctt ttattttatc tattaatttt aaatacgcat
    19741 ctttatctcc acaatactct aaaacagcca tttcaacttt catttgttca ccaattctta
    19801 catagtcaac ttttttcatg ttagctattt tagcatctac tgcttcatca ctcatacaag
    19861 tacagaatga aactgcatat ctatttctac ttactaatgt tttttcatgt ctaaataata
    19921 ctgtttctcc acctagttca tactcagatg caccagcacc aacttttaaa gctttcatta
    19981 aaggtgcagt agcctctgat aattttgcta tagcgtcatc agacatatgt gggcatttac
    20041 ctacttctac agctcctgaa gcaaccttca tagagaaagc catacaagtt gggaatccac
    20101 aatccttaca gtttttcttt ggtgttaatt taaatatatc taaagctttt aatgccatta
    20161 ttttttcccc ctaaccttaa caatttaatt aagctaatgc gttaaccaat tcttttatag
    20221 tttctattga ttttggatga cgaagtataa ctgcgtttga accacctact aaaacacttg
    20281 ctgcagttga aacttccatt gctatagttc tttcttctgg acaaccccaa tctggctcat
    20341 cttctattgg agctatagct tctttaacat gacctacttc aaaagctact ggtgttatga
    20401 taggcatttg taatgtctta tcattttgac caaatgctgc aagtctaatt ctatccatag
    20461 tagatgcaac gtactcatat ccataaccaa ctgctgaaca tccaacattc ataactatat
    20521 tttcaccttt aacgcctagt tgagttaaca atacgtttaa ttgtttagct aggtttatat
    20581 caacagaaga ttcagctcct actttatgtg cataagccat accagctgat gctcctactc
    20641 ctttgtagtt atcttctact gctgacataa ataagcagtt atgtccatcc aatgtttgag
    20701 ctaatttttc aaataattta ccatcttttt catgatttcc tgaaccagct acaactaaag
    20761 gtaattttat tgcttcaatt actgccttag ctacatcagc acattcatca actgacttat
    20821 ctaatccatt tgggtcagaa ccatcaaatt ttaaacaaat aaaatctgct tgtgtattag
    20881 cttcaacata tttagcccac tctactggac aattagctac atctttgtat aattccttat
    20941 atgaatcagt ccagctttca ggataaacat ctgatatttg tatccctatt tttggagaat
    21001 tacctacttc tccatcaaaa ctataaaaag gtaatacatt ttccccacct aatttaattg
    21061 ctttttcccc tattcctact tcaacttctg atatttttcc agaatatttt tgagtagaca
    21121 ttttaaatgc catatttttt tcccccttaa gcctatttta attctaattt agcaaaaatt
    21181 tctttcatag ccactttaga ttttgaatct tcaggtaaat taactaaagg aatacctgta
    21241 gaatcatatt catatatcaa ttcatccata ggtacaacac cgattagctc taggttatgc
    21301 ttttcaattt cttctttaac accatcattc ataaccccat ttggaacttt atttacaatc
    21361 aaaagtattc ttccaacact taactttagt tcctcagcta aatctcttat tctagcaaca
    21421 gcttgtatgc tacgtcttga gcaatcacta accaataaaa gtgtatcaac atgtctagtg
    21481 acttttctac ttagatgttc cataccagct tcgttgtcca tgactaagta tttgtaatga
    21541 ccagatattt tgtttacttg ttctctaagt atcccattta caaaacagta acatccttca
    21601 ccttctgacc tacccattac taataagtca tatccttcgc cttcttcgat tatagaattt
    21661 aatctaaatt gaagatattg tgcctttgtc ataccaccag ggaatgcatt gcctaacttt
    21721 tctctttgat ttacttcttc tcttatttct cctattgttg cttcaacttc gatacctaat
    21781 acttcattta tgttagcatt ggcatcagca tctacaacta atactggtcc ctttttgtcc
    21841 ttaaccaaat aatcaatcaa aagccctgtg agacttgttt taccagttcc accttttcct
    21901 gcaacagcta tattgtatcc cattttaaga cttccccctt aaatctagcc acagagcctt
    21961 atgtactctg tctgtctatt ataatttagg tacagaatga acacattctc catgtacatc
    22021 atgaagtgct tccattagcc cttcagataa acttggatgt ggatgtattg catcccctac
    22081 ttgttctact gttaatccta gatgaacagc aagtgacaac tctgtcaata aatctgtagc
    22141 atgaggtcct actactgctg cacctattat cttatcagtt tctttgtcag ctataacttt
    22201 tacaaatcct tggaaatgtc ctatagcttg tgcttttcca agacctctaa aatcaaattg
    22261 acctacattg tattctacac cttcagcttc aagttgcttt tcagtcttac caacaccagc
    22321 aacttcaggt tcagtataaa cacatcttgg aattgctcta taatctacta ccttagtctt
    22381 acctaaagca ttttcaactg ctacgatacc ttctttagaa gcaacatgtg ctaggaaagg
    22441 agtatctatt atatcgccta ttgcgtatat accttctaca ttagtttcaa gatgttcatt
    22501 aactactact tttcctcttt ccatttcaat tcctatatct tctactccag aattgtcaag
    22561 gttaggtctt ctaccaacac atactaatgc atattgtgct tctataacct ttccattaga
    22621 aagagttgca acagccttac catcaactac ttcacaagtt tgtactccaa tacctgtaat
    22681 aaccttaatt ttatctttct taaattgtct aagtagttgt ttagctacat ctttgtcttc
    22741 atttaatagt atttggtcaa ccatctcaac tatagttact tctgttccca aggctctaaa
    22801 gaactgccct atctcacatc ctataactcc accacctact attaacatag actcaggtat
    22861 ctcttcaagt ccaagaactt catcactagt tattacaact tttccatcat atgggaacat
    22921 tcttggtaca actggaacag aaccatttgc tagtattatc ttatctgctt ttatagtttc
    22981 tactgttcca tcatctttag ttacttctat agtgttttta tcaattaatt ttccaaaacc
    23041 attaactaag tttactcctc ttttttcaaa taagaactct ataccactta ttaattggtt
    23101 tacaacttta ttttttcttt ccattatggc agtgaagttt ggcttaactg taccatctat
    23161 ttctatacca aaatcctttg cttcttttac agtattcaat actcctgaag aagcaagaag
    23221 tgctttagtt ggtatacatc ctgcatttaa gcaagttcct ccaactcttc ttttttcaac
    23281 aactgttaca tctgcaccaa gcatagaagc ttttatagct gctacatatc ctcctggtcc
    23341 accacctact actactatct tcatctttaa ttcccccaaa cctaatataa tctacaatct
    23401 tgcaatacta taaacctgcg gcatccagta tagcaggtat attctcttct gccccaattg
    23461 ccattatatg aacaccatca cataaatctt cttcttttaa ttgtttgata agttcccctg
    23521 ccatttttat tccttcactt acccagtttt cttttccagc tgctcttaat ctttcgattt
    23581 gttcatctgg aacaaatatt cctggtacat ttgcagtcat aaatttagcc attcctggtg
    23641 attttaaagg tactattcct gctaatacct tgcaatccat atcccttgtt aattctctaa
    23701 actttctcat tgtatttatg tcataaagtg cttgtgtttg gaaaaattta gcccctgctt
    23761 ttattttctt tttcattttt aataattgaa cttctattgg agaatattct ggagttactg
    23821 atgctcccag ataaaaatct ggagaacctt ttaacttatt accagccatg tccgtacctg
    23881 ccattaaagt ctcagcagtt tgaagtatcc ccacagaatc taaatcaaac actcccttag
    23941 cttgtggatg gtctcctacg ctagtatggt ctcctgttag tgccaacaga ttgtttatcc
    24001 caaatacacc tgctgacaac atttctcctt gtatagctat tctatttcta tctctccctg
    24061 ttatttgaat aactggctct aatccagcat cttttaataa tttacatgta gcaagagaag
    24121 ttgctctcat aactgccgat tgaaagtctg ttacatttgc tgcatgaact cttccaacta
    24181 acggctttgc acattctatt aaatgtgaaa ggtcagtccc ttttggagga gccatttcag
    24241 tagtaactgc aaattttcca ctttctaatg tttctcttaa taagctcatc tctaatcccc
    24301 cttttaacct tctaaaaaaa acttttatga atttgctgct gcttctttct ttttctttgc
    24361 acttaaactt cttggattat tttgcttaga atagtctttt ggtggtctta tttctgctaa
    24421 gttatctaat tgtcctatag cttctagtct ttcatatatc tttatccaag cacaatcatt
    24481 ctctgagttt acttcacact taccattttt agctccacca caagccccat tcataagacc
    24541 tttagcacac atagttactg gacatatccc acctgtccaa ccaagttcac agtctccaca
    24601 agccttacag gcttcttcat attctccaac tctttgtact tcccctataa acaaagtatt
    24661 gttggctgga taaactggct tatcttttaa attcttaact attgtctgtg taccatctcc
    24721 acatgctaaa gataaaactg catcagcttc cttagtctct tcctttagag cctttaaatc
    24781 ctttttagac tttaaaaggt tacaagcagg gtcaagcatt acatatccta agattttttt
    24841 gccttcacct tcaagagttt ccttcatttt taaaacttct tcttccccac cacttttaca
    24901 agttgctgca cattgattac aaccaactaa aaccagcttg tcaaaatctt ttaagtaccc
    24961 tagcacttct tctaatggtt tattttcaga aattatcata tctacttccc ccctaaacct
    25021 tccacatata tatatctatt tattgttttg taatttacaa gccttaacta catgttttgc
    25081 aagtatagat gttgtaactg aaccaacccc cgctggaact ggagttatca ttgatacttt
    25141 gtctaaaact gcatttgtat caacatctcc acataaattt ccttcttcat caacatttat
    25201 accaacgtct ataactaccg ctccatcttt aacaaaactt tcatcaacca tcttagctct
    25261 accaatagct gctattagca cgtctgcctc tgctgctaca cctgatagat ttttagtttt
    25321 tgaatgacat attgtaacag ttgcatgttc acttaataaa agcattgata ctggctttcc
    25381 aactaccatt gatcttccaa gaactgttac cttacttcct tttaaatcaa cattatagtg
    25441 ttttaatatc tcaactactg ctgtaggtgt acatggtgga aatccacttt tatccccttc
    25501 cattacctta gcagagttta ttggactaaa acaatctacg tctttttccg gtgcaataac
    25561 atattttata acttcttcat ttaagtgttt tggaagaggt ctaaaacata atataccatt
    25621 aacatttttg tcatcattaa ctctctttaa tacatcaata tattcctctt gtgttatatc
    25681 ctcagctaac tctaacactt ctgttgttat acctatgttt tgacatctct ttagtgctcc
    25741 tctttcataa gataaatcat cacttcttgc cccaactcta actattgtta gtttaggatt
    25801 tataccttct ttaattagta aatcgacttc ttttattaat tcttcactaa tcttatcagc
    25861 tacaggtttt ccttttataa tttgtccttt agttgacatt ccttccatac attcacaact
    25921 cccctacaag caaacttaaa ttaatttatt ctatatacca catattaata acatagtatc
    25981 tactttcgtc atttaaaaat tattttccaa gagctttttc aactttggca taaacttcat
    26041 cacaaatttt tacaccatct tcaactaact tatctacttc tgtttttacc ttattaacat
    26101 attctttatc ttgtatagaa tttatattta taactacatt taattgtcca ccaagtatag
    26161 cagctcttaa acactgaact cctacaccaa catcacttat agcaagtctt gaacctttat
    26221 caactaaatc ttcatgtaac ttaatagctt catagcaaac tcttactatg tttaatggta
    26281 cttcacaagc aacttttaat gccttttcca ttgtttctgc ttttattctt ttttcttctt
    26341 ctgtttcttt aggaagcccg taagcttttg ataatggtaa gaaacattct gcatcatcat
    26401 ctatcatttt taataaatct ttttctaact ttcctgcctt acttaatatc tcttttacac
    26461 tttcttcata ctcagcatac ttcttcttcc ctattgtaag gttacatacc atactaccta
    26521 aagccatacc tattgcacct actagtgcag cagctccccc accacctggt actgcttctt
    26581 tggaagatag tacttctaca aaatcaacac aagttttatc tgctattttc atgataatcc
    26641 tccttgaaag gatgtaggaa aatatctcaa attgttcgag atattttacg aactatttta
    26701 agatatctcc tactctttat ttataaatta atttccttag aataatccag ctattactcc
    26761 attttcatca acatctattt tttctgctgc tggaactttt ggtaatcctg gcatcttcat
    26821 tatttctcca gtaagtgcaa ctataaatcc agctcctgct gaaattgttg cttgtcttac
    26881 agttattcta aatcctgttg gtctacctaa tttagtttgg tcatcagtta aagaatattg
    26941 agttttagcc atacatactg gaactttagt aaatccaagt ttttctaagt tagctatttc
    27001 cttatcagct tcaggagtat aatctacacc atctgctcca taaatcttag tagctatagc
    27061 atttatcttg tcttttatag ataaatcatc ttcatagcag aatctaaagt tattttcttc
    27121 ttcatccaat aatcttaaaa cttctttagc tactgcaatt ccaccttctc caccttttgc
    27181 ccatacttca gaaagagcaa cattaacacc taattcctta cacttgtctt caacaagttt
    27241 aagttcagct tcagtatcaa gtgggaatct attgattgca acaactgctg gtaatccata
    27301 tacttgagtt atgttttcaa catgttttaa taagtttgga agtccttttt caagagcttc
    27361 taggttttcg ttatttaagt ctgctttagc aactccacca ttgtatttta atgctcttac
    27421 agtagcaact attataacag catctggttt taagtttgcc attctacatt ttatatctaa
    27481 gaatttttct gctccaaggt cagcaccgaa accagcctca gttattacat aatctgcaaa
    27541 gtgcatagcc attcttgtag ctataacaga gttacatcca tgagctatat ttgcgaatgg
    27601 tccaccgtgt acaaatgatg gtgttccttc aagagtttga actaggtttg gttttaaagc
    27661 atcttttaat aatgcagcca tagcaccatt agcttttaat tgtctagctg ttacaggttc
    27721 accttcgtag ctgtatccaa ctattatgtt tcctaatctt tcttttaaat cagatatatc
    27781 acttgccaaa cagaacgctg ccattatttc agaagcaaca gttatatcga atccatcttg
    27841 tctaactgct ccatcacctt ttttacccat accatcaacg atatttctaa gttgtctatc
    27901 gttcatgtct acacatcttc tccaagttat tttcttaggg tctattctaa gtgcatttcc
    27961 ttggtgaata tggttatcaa gcatagcagc taaaagattg ttagcagctc ctatagcgtg
    28021 aaaatctcct gtgaagtgta agtttatatc ttccatagga acaacttgtg catatcctcc
    28081 accagctgct ccacctttaa caccaaatac tggtcctaga gatggttctc ttaaagcaac
    28141 taatacattt ttgtctagtt tagcaaatgc atcagcaaca cctatagtag tagtagtttt
    28201 tccttcacct gctggagttg ggtttatagc tgtagttagt attaacttag cttttttacc
    28261 agtctctctt tttagtagat tgtagtcaac ttttgctttg tatttaccat aaagttctac
    28321 atcatcttca cctagtccta atttttttgc aacctctcta atatcttgag gcttagcttc
    28381 ttgagctatt tcaatgtcag atttaaatcc cataactgaa tctcctccca caacctaaat
    28441 agatttaaaa tataattttt gtaaaaattg tattaatatg taaaaattat attttataag
    28501 ataattatac atgtaaaatt acttaaaaac tagctactaa gctattaaaa tattcaaact
    28561 taatagctag ttatttatct aattacttac ttattaccta tctattgcct atgtctaacc
    28621 tttaaccaac ttaaaatccc aaatcaaaat ttctatctat tttacttatc cttaagtgac
    28681 attaatttat cttttattgc tttaatctgt tctttcgttg tttcactaga atcatatgga
    28741 gattgattag ctctatcaga atcaataaca tcttggttat aataaataaa tcctaaagat
    28801 tctccatctt ctaagttttg gattataaat tcttcatctt ccttatttct aattttattt
    28861 cctactacaa atactttatt tacacctata tcatgtccaa gtttcttaac ttttctataa
    28921 gtctgtagac ttctctctcc tggttctaca actactataa aagcatctac accttgggct
    28981 gttcctcttc ctaagtgttc aattcccgct tccatatcca taaccactac atctttattt
    29041 tgtagtatta aatgtgagca gagtctcttc aaaagaacat gctctggaca tacacatcca
    29101 gtaccaccag aatctacggt tcccaatgta agtaatctta ctccattgta ttctttacag
    29161 aaattttcag ggatatcgtc tactttagga ttcatcttaa acatttttcc aaatgaacct
    29221 acagaagcag ctgttctatc tgaaactaat tttttcattt ctgatatagg tactattgat
    29281 tcatatactt cttttggaaa acctagtgct aaagctaaat tagcatcagg gtcggcatca
    29341 acagcaacaa ctctgtatcc gtcttcagca aacatcctag agagcattga agaaaaagtc
    29401 gttttaccta ctccaccttt acctgttatt gctatcttca taatccctac caccttttat
    29461 tactatttgc tttttagtag ttaattttac agttaaatat tttttatact ctatattttt
    29521 atacactaaa aataaactac agctagtcct tacaggaacc aattaaattt accctactag
    29581 cttttcaaag cagcatgtat agttttatta ttctttaata ttgtttatat tttatttatt
    29641 cagtatattc attatatttt gatgttcttt actttaatat ttgactttat tcaactttat
    29701 tcaaccagaa acttcttata aaaacgttta ttctataact tcactatttt ttaagcttca
    29761 gccttttctg ccattttttc ttcaactaat gcttcaaagt gaacacgttt tttctctata
    29821 tcttctatta ttgtagctgc taactctttt ggatttgtat ttacagtaaa tgtagctcca
    29881 actttatctt ttaattcact agttaatata tccactgctc ttgaagaacc agctattgga
    29941 ggcattattc ctaaataagt gtctatacca gaagctacaa catagcatcc tatagcaact
    30001 gctttttctg acatccactc tggagctata cctacaactg gaaggtcaca catatccata
    30061 tctaagtaat tagctgctgc tccaactaaa tctagtatac ggcttatatc aacacaagaa
    30121 cccatatgta gtactggtgg gattcctact aatttacaga cagttgcaag acctttacct
    30181 gcatattttt cagccgcatc agattccatt aaaccaaact tagctgctgc tgaagctcca
    30241 caacctgtag taactactat tatgtcgttt ttgattaatt ctttcattat tgttacgtga
    30301 gcctcatttg aaactccttt agcattgtta catccaacta caccagctgc tcctcttaat
    30361 actccagctt ttaaacagtc tgttaatggc ttaactgttc ctgctgggtc tatatgagag
    30421 tttacaactc tgtctaattg atttactact gcttctacac tatacccaac tgttgcgcct
    30481 gattttaatt caggtataag tacttttgat ttatctctat ttttaaagtt taaaatagct
    30541 tctttaacta ttgctttagc atcatcaagt gcttgttctt ctctaaattc aatataagtt
    30601 gaacctgtta tcttagcttt tggagaagtt gttacaaatt tagtatggta acaatctgca
    30661 actctagcta atgcagggaa tatacattga acgtcaacta ttacagcttc aactgctcca
    30721 gttattattg caagttcttg ttgatggaag tctcctgcta tttttacacc atgtctcatt
    30781 gtaatctcat tacctgtaca acacatacct gctaagttta ttccatcagc accaacttct
    30841 ttagctaaag ctactaagtc tggctcttct gatgctaaaa ctatcatttc tgataatgct
    30901 ggctcatgtc catgtaatat tatgttaact ttattttctt ctaatactgc taaattcgcc
    30961 tctgttcttc ttggcactgg agttccaaat aagatatcgc ttagacgagt acctatcata
    31021 gaaccacccc aaccatcagc catagaagtt ctaagactca tgtgtattaa actatctata
    31081 tcaccagtac atcctatatg agtagagtgc attattgttg caatttctct atctatagct
    31141 cttggctcta tagcgtattc tttccatatt ttttgtcttg gttctggtgc attttttata
    31201 aatcttaatg tcccaaaagg ttttccaaat tccattaatg caatttctgc cacttcatgt
    31261 gctacatcat agatatctct accttctgtc tctacatccc attctttagc taaagtaatt
    31321 aacttagctt cgtctcttat tttatagttt ccatttggat cagctaatgc taatgtatgt
    31381 gctatatctc tagcatggtc tgaatgggca gctgtcccac cagcaaccat tcttgcaaag
    31441 tttctaccta ctatagtatg ctcatctgct ccacataacc cccttggagt cttagggctt
    31501 attctacatg gtcccattcc acatattcta cagcaaacac cttgaagacc aaaaccacat
    31561 tgtgccttaa agccttcttt tctatcatac attgtttcta cgccttcttc tctagcttta
    31621 gctatcatct tctggctgtt taaatcaatt gttaacattt tttcatccat attcctatcc
    31681 cctttttttg atttttaaaa tcccccaggt gactgacttc atgtcataaa ataataaaat
    31741 aaatgctttc taagttgatt atatactaat tttcagtgct ttgctttaga tttaaatatt
    31801 tttttaacaa aattagataa tacataaaaa ttttattttc tacacttaat ttaagttgat
    31861 tttatcgctt gtttttggct atttgttaat atctaaacct acttttttga tacttcagtt
    31921 taattaaatt atgttttgat ttttttaaat caaatttgaa atttttctag tcatttttat
    31981 attattgcag attgtttgtg tatttattat ctttcactac tgtttggact tattacagat
    32041 ttttactccc taaacttaat tagtaaaaat ttcttagaat agcatttttt ggactaattt
    32101 ttttgaattt ttttattttt cctactttaa tattaattat agcttcaaat tgaacatttg
    32161 tatatattca tttgaagcta taattatttt ttatataaaa cttttttaat acagcattaa
    32221 tttagacatt ttgactattt tttctacttt aattcattta tttcaagcga tttatcctat
    32281 ttcatatttt tagctacaat agccccatat gcaatagaat ataatttaga ttcatgtgaa
    32341 tcagctcttg acactaaaac tataggagct tttgcaccca taattatacc tgctgattta
    32401 gagtctgcca tatatgtaaa ggctttccct attccattac caacctctat tgttggaacc
    32461 aatatgatat ctacatctcc agatacttca cttttaaacc cttttatctc actagcttct
    32521 tttgaaacag ctaaatcaaa agccagagga ccttctacaa ttacattttc accaaattct
    32581 ccttctttac aagcttttgc taacatatct gcatctacag ttgcttgcat cttaggattt
    32641 actttctctt tagctgccaa acatgcaact tttacagttt ccattcctaa agcttttgca
    32701 gcttttatag aattttttaa tatctttgcc ttttgttcat aatcaggagc tatattcata
    32761 cctccatctg tagttataag taatttatgg tatttttcta actcatatac cataacatga
    32821 cttaataaac tatcagttct aagaccatag tctttattta acacttcttt taatatgaca
    32881 gatgtatcta ataatccttt cattacaaag tcagcttctt tacttgatac taatttaact
    32941 gctatttctg cacactcttt gatatcatcc ttatccacta ttttgatacc acttaaatca
    33001 aatcctattt cttttgatat ttctcttatt ttgccttcat ttccaaccaa tataggagta
    33061 attatatttc tttctacagc atcctttatt gccattaaaa cttctttatc atgagctgct
    33121 gctacagaga gtacaatttt ctctcctccc ttaagttgtt ctaaaatatc atctaatttt
    33181 tttatcaaaa gtaacccctc ccttatatat atttaactaa ataataccat atttgctata
    33241 ccttttctca atttattttt tatgttataa agaatttata taactaaaag acagtttaat
    33301 attttattct gggatttcat caatagctct taaaagcttt gcaggaacac cacctacaat
    33361 agtattttct gcaacatttt ttgttacaac tgctccagca gcgataattg cattatctcc
    33421 aatagtaact cctggaacta cagttacatt agcaccaatc catacatttt ttcctattgt
    33481 gattggagaa ggataagttg taccacgttt ctctggtgca ataccatggt taagtgtagc
    33541 caataccaca ttatgcccaa tttgcgtatt atctccaatc tcaatccccc cctggtcttg
    33601 aaaatggcaa ctgctattta taaaaacatt ttttcctatt gtgatgtttt ttccacaatc
    33661 tgtataaaat ggtggaaaca taaaaaatga ttcatctacc ttttttccaa ttaactcaga
    33721 aaataaacta cggacttctt ctggttcatg ataagaagaa tttaacttac ttgtgatttt
    33781 cattgcctca ttactcaact taaccataaa ttcatgtatt tctgatttac tagaaacagg
    33841 ttttctttga ttgatatgtg ttaaaaattc attgatattc attttataca tcctttcaat
    33901 atgtgcttta atttatttgt acattctaaa taatatttta ataaaaatta atttaattta
    33961 tttttattaa aatatcattg cttcttttag tgtaatatac aattatatct atatcaaata
    34021 tctatattaa atacttaact atacttaaaa agcatataaa ttgatataat tatataataa
    34081 ataagttaat attgaggagg gataattatg gaaataagag tattaagata ttttttagca
    34141 attgcaagag aagaaagtat atctggtgcg gcaacctttt tacatataac acaaccaaca
    34201 ctttctagac aaattatgga acttgaagaa gagttgggag ttaaattatt ggttcgagga
    34261 aatagaaaaa tcaccttaac aaatgaaggt aggcttttac ataaaagagc agaggaaatt
    34321 atatcattag ttgataaggt tgaattagaa tttgacaatc ccaatgagat tattagtggt
    34381 gatatatata taggaagtgg tgaaactgaa gctatgcgac ttatagctag aactgcccat
    34441 cattttcatc aaaaataccc caatgtaaga tatcaccttt ttagtgggaa tgctgatgat
    34501 gtgactgaaa aattagacaa gggactgctt gattttggta ttttaattga acctgcaaat
    34561 atacagaagt atgattatgc acaaattcct gctactgatg tatggggttt actaatgcgt
    34621 aaagatagtg cccttgcatc acaaagcaca ataaatccaa aagatttatg caatatacct
    34681 cttattattt caagacagac acttgttgat aaagcaattt ctaaatggct tcaaggcagt
    34741 aatgaaaaac taaacattat tgcaacatac aatttagttt ataacgcttc tcttttggta
    34801 gaagaaggtc tgggctacgc tctgtgttta gatagactcg tcaatactac agaaaacagt
    34861 aatctttgtt ttagacctct atctcctcct ttagaagcta aattaaatat tatatggaaa
    34921 aaacatcaaa tattttcaaa ggcagcagaa aaatttttac ttcaaataca aaaagatttt
    34981 tcttctatca tgttggatac atctaagttg gatacaccta aagaaaaata aatcataata
    35041 aaaagtgaga gtgtctcaaa atgaactttg cactgtataa ggcactcttt tttatacaaa
    35101 gtaaaatata tgttcatcat ttcagcacta aaaaagaggc ttatctctat tttgagatag
    35161 ccccacttaa ccaataaaat taaatacaac ttaacaatat ccttacttat ttattccaag
    35221 ttctcttaga caagcaatcg taatttcatc ttgtggattt aattcataag ctctttctat
    35281 atagtaagtt gcatcatcaa tatcaccatt atttaaataa gccattccta aattgcaaag
    35341 tatttctggg tcttccttta tcttagccgc ttgttcaaaa tactctattg ctttatctaa
    35401 attattctta taagcctcac aaagccccaa ttccactata gtatccacct gattaggctt
    35461 tattatcaat atcttctcaa gatacatctt agcctgctct atttcgccca tacccttata
    35521 tccaagtgct atcataaaca gcaagttcca ccaatccata tgttcttctt ctaatggaag
    35581 taacttctct aatccttcct taaacttacc ttggaataca agtgtatatc cttcttcata
    35641 ttgaacttta aagtccatct tgcccagatt ctcttgtacc tcagctacta aatcagcatc
    35701 taaccctagc cttaacgctt cttcccaagt cagcttagtc tttaaatatt gaccttgatt
    35761 ataataatgg tagcctaagt gataataacc taatgcaaaa ttctcatcaa catcaattac
    35821 cttttcaagc ttagccatgg cttcaagtaa gaatgcattc attgccttat catccatatc
    35881 tttttgatac tgtttagcta tctcttggca aacaatcgca tagttataca ttgcatctaa
    35941 atcatctgga tacattgtta taaatgcttt tatatatatt aaagaatctg tatgttcacc
    36001 aatctcaaag tattttctcg acatatatcc catatacgat ttcaaatcca aatcaatatc
    36061 ttttgataaa gcatttaaaa acttctcata ctctgcattg tttttaaact tactatctat
    36121 tccaattata tatatcattg catctgctat agacattgaa tttaacttat cttgagcagt
    36181 cttttctttt atccctttta ctaatacctc attttttata ggtacatcta atccaccttt
    36241 tggcacttca tagcttttta atttaaactc tccaccatgt tttatagtta taaaagctaa
    36301 ttcttctgct ttttttagca aatatttttc tatcttaaaa tccattttat cctcctaaat
    36361 acttaaatct ctactttatg agttttagat ttctcaaata tttttttatc atcatactca
    36421 aataagacta cattttctac ttctcttatt tctgtattta tatcattttt taaatccaca
    36481 acattaaatc taaatggctc aaaatgaaca tattttatta tatgtttagc tggtttatca
    36541 acaaaactag gtaaatatgt tgataagtta tcattatctt gtaaaaatac atggcatcta
    36601 ttcttaaaat catccacatc tagcttatta aaaaactgtg gtatatttga tgttttaccc
    36661 agtgatatat agtcatattt taatatatcg ttgaatacat cattatctat gttgattttt
    36721 tcattataga aatccatcaa tattttatat aactgattct ttccttgtgc caaatcaaag
    36781 tagccatttt catcaaaata cttggcaaaa acctcaaaga atttaaaagg gcttttttta
    36841 taaaatctgc ctataatata tctcatagat aacacaaagt ttttagaatt gtagtatctc
    36901 tccagtatat cttctatatc ctttagcttc aaagtttctt catagcttat ataattattg
    36961 tataaaaact catatggagc ataatcctta tatttaaaac catgctcatc tgcaactttt
    37021 ctcatacctg tccctttaat catctttaaa aagcctaatt gcagatgctc tatacctaga
    37081 ttaaatacat cattaaaaga attttcaaag ctcttataat cttcataagg cagtcctgca
    37141 attaagtcta agtgttgatg tatatttcta taagatgcta cagtttgtac aacatgagac
    37201 aactttttaa aatcatccct tctacctact gcatcaagtg ttttttcatt cgttgactga
    37261 acccctattt caaattgaaa aagaccttct ttacaatcag ccaaaaactc aagcatctta
    37321 tcatctatca aatacgctgt aacttcaaaa tgataagttg tatatccatt atcatttttc
    37381 ataagaaaat ccatgatttc catagccact tttctatttg cattgaatgt cctatctata
    37441 aactttattt gtgatactct tgcatctatt aaagctttta aatcactctt tatcctgtct
    37501 aaactaaaat accttagacc tggaatagta gatgataaac agtattggca gttaaaaggg
    37561 caacctcttg atgattcata atacactatt ctattttcat attcttttgg attcaagttt
    37621 tcataaggac taggaatctc atccaaatct tgaagtaatt ccataggctt attcactatt
    37681 atctcattgc cttgtctata tacaagtcca tttacatcat ttatttccat ctttccttgc
    37741 aagtgaagta ctaaatctct aaaaattaat tctccctctc cataaagtat ataatccaca
    37801 aaatcatact ttttcattgc ctcataacta tcataactca cttccggtcc gcccaaagct
    37861 attttagtat taggtctaat tttttttaga ttatcacaaa gtttaactat atctcccaca
    37921 ttccagatat atgttgagaa taatattata tcatattcat ttttatatat atcctttaaa
    37981 atatagtcca aatcgttgtt tatcgtgtat tctttcatat taacttctat caaatctctt
    38041 acaaattctt ttagatatct tatagccaaa tttgtatgaa taaacttgga atttaatgtt
    38101 gttaataaaa ttttcaaata tttctcacct ctttttaaga ttatactgtc tttttgtacc
    38161 ccttataggt taaaatagta tatgtaaagt ctaaataata tctatataat taatttatta
    38221 atttcaagta atgtgaggaa aggaagtttt ttatggaatt aattgaaaaa gtatctatca
    38281 actctttatc aaaaagagat ttactactta ttataaagtc tcttgaatac actaatgaaa
    38341 atactcaaat tagtgatttc attgacctta ggaataatat agtaaaggaa ttatgttttt
    38401 taactgacac taaggaaaaa gattttttag attatctaga aaaaaatagc acactctaat
    38461 aacctaaacc tttttaaact ttaatatata aatacctgat ttagaattta tttttatgct
    38521 taatatatta tcattaaagg ttttcgacat aactgttatt tgattaaata tagggtctac
    38581 tatagtatta ccttcttcaa gtcttataag acttcttggg tctatgcttt tattatatgc
    38641 cttaactcca attttatatt ttctcaattt aacaacatat tcggacatag aaaaaataaa
    38701 atctatatta tcttcattta atttactatc ttcacccaaa atggataata tagatttttt
    38761 tatgctatta attccttcat tttctacagt tctatcttcc atatatacat catatagaac
    38821 atttaaaaca tttaataaaa tactttcttc atcataatct tcagatttca aatcaatgta
    38881 cttatcaatt aattgtattg ctttaatcct tgccatctga aactttacaa catctgattt
    38941 gtctaattct atagaaaatc ctattatctt gtctttaata ctctgtagta attcgacata
    39001 ctctatttta ctattattta taagattgtg cataatggaa ttatacacca cagaacctaa
    39061 taaataatca aataaaagtt cttctttttt aaaataaata acatactttt ttattagttt
    39121 aataacctca tcttcacttt tttgaatatt agtatttgat aaaattagtg gtaataaaat
    39181 atttaaaaaa ttgatatttc ctattttttc tccataaaat ctatagtaaa taagttcatc
    39241 tctcagtcta ttttcgtcta tatacacaca tctttgcgtt ttatcatcta ttaaggattt
    39301 tgtatttatt atagttaaat ctttaagagc cctacttgca ctactaaaag aagatgtgta
    39361 gacttttttt agtaattcgt cattcataaa tatatccttt ctgaaaaatt gaatgtaaat
    39421 aaattgttat taaaaaaaaa gagacagccc gtttaagaag ctgtcatata tagggggata
    39481 gtagtgtaca aaaatcattg taatctacta tctatatatt attatacgat actacttttt
    39541 tatatgtaaa gcatcagttt tcgacatccc tatattttac agttatacaa attgcgtact
    39601 aagttcaatt ttattctttt tatttcaaca ttttatcatg ctacattctt tcaggagcgt
    39661 gcattcccaa caatttcaga gaattttcta aagtttgttt tgttgctaaa actaatgcaa
    39721 gtcttgcttt tcttatttcc aaattttcta ctaaaattgg attgtcatga taaaatttat
    39781 taaacgcttg agctaaatct agcataaatc tagtcactat atgtggctca tttttcttta
    39841 gagcaagtaa aatattctta ttgaatgatt ctataactct aagaacttct gcactatctt
    39901 catcagctaa tagactataa tctatatcag aagtcacttc tacttctgct tttcttaaaa
    39961 ctgcacagca tctagcatgt gtatactgaa catatggtcc agtctcacca tcaaaactaa
    40021 gcgttctttc ccaagagaag gtatagtctt taattctact attagataac tcttggaata
    40081 ctactgcccc aacacctact tgcttagata tttcatccac atttttagca tttgggtttt
    40141 tagctagtat aatttcctta gttttatcta tagcttgatt taaagcatct tctaaaaata
    40201 ctactcttcc ttttctagtc gacatagtac cttcttcaag tgcaaccata ccaaatccaa
    40261 catgaattaa atcttttgcc caatcatatc ccataagctc aattacctta aaccattgtt
    40321 ggaaatgtaa gttttgttga gaaccaacta catatataca cttgtcaaaa tcataagttt
    40381 cttttctata tattgcagca gctaaatctc ttgtcatata taacgttgaa ccatcattct
    40441 tagtaataag tgctggtggc attttatact cttctaaatc aacaattctt gctcctttag
    40501 actcttttaa aagatttttc tcttctaata gttcgataac tctgttcatt ttatcagaat
    40561 aaaaactctc tcctgctaaa gaatcaaatt ctatatctaa aagtttataa actctgttaa
    40621 actcttttaa actctcattt ctaaaccatt gccataattc ttgtgcttct tcgtcaccat
    40681 tttctaattt attaaaccat gctcttgctt catcttccat ttctgggtgt tgttcagctt
    40741 catcatggaa tcttacgtat aattttagta attctggaat tggattagac tctactactt
    40801 ctttttctcc ccacttttta aaggccacta ttaacttacc aaactgagtt ccatagtcac
    40861 ctaaatgatt tattcttata gtcttgtatc cttgcgaatc atatatctta tataaagcat
    40921 tacctataac tgtagttctt atgtgcccta tatggaaagg ttttgctata tttggagaag
    40981 aatattcaac tattacagtt ttatcttttc caaaatcact atgtccgtaa ttttcttttt
    41041 catctaaaac ttttttaatt acagtttctg ctaattgaga tttatttaca aagaaattta
    41101 catatccgcc taattgtatt acttttgtta tctctccact tggttctata ctttccgcca
    41161 attcacttgc tatcatgttt ggagcttttc taaacacttt ggctaattta aagcatggaa
    41221 aagcatagtc ccccatatcc ttatttggtg gaacttctat aagtgcctct atctcttctt
    41281 tcgataaatc ttctattttt tctttaaggc aatttgatat tgccacctta aaatcttgca
    41341 ttctgttgct cctctctaca ttgttcctgg tttatattgt gcttatctct ttatgttaaa
    41401 tcaaatttcg tgttttttca agtatttcat tacagtgtaa aacactttag taattatgtt
    41461 ttctgtatat attaaaaata ctataatttc agtttttaat cacactatta ttgatttttt
    41521 ataagacttt tttctatttt attgtttctt gtactatcca gttgacttta tatacttttt
    41581 ttaatctttt atctgttttc atacatcaat gtcagcttta tatacatttt tttaatcttt
    41641 tatctatttt catacatcaa tgtcagcttt atatactttt tttacttttt tatctatttt
    41701 tatacatcaa tattagcttt atatactttt tttactcttt tatctatttt ctataaatct
    41761 ttttcactct taacttttat tccttcttct tctaatagcc ttgctgttat acctttccct
    41821 ggtatctttt tacctgaaaa actaccatca tatattagat tacatccaca agaggggcta
    41881 ccctctttta gtattgcttc gctacataca tataaatttg ctatttttag agtttcatta
    41941 gcccctctta caaagttttc tgttacatcc ttattatctt tagtaattac ttttccatca
    42001 actatctcag caggtggtct tggtgtagat agaccaccta actgctctgg gcatacaagt
    42061 gtaaattctt tgccttttaa atattccttg actttttcat tatcattatt atcgccacta
    42121 tatttacaat ttattcctat taaacatgca gataccaata tcataaaaat gtgctcctat
    42181 acaaattata tatattctca tacaaattac taatgtattc ttatacaaat tattttaatt
    42241 taacaattgt tactcctgct ccaccttctc catattgacc acctctttgt gattttacat
    42301 gtctatttct tcttaaaatg tcttgtagcc ctgcttttaa tactcctgta cctattccat
    42361 gtattactgt tacagactct aaacctgcca catatgcatc atcaagatac ttttctactt
    42421 ccattatagc ttcttctagg tttaaccctc taaggtcaac ttcatttttt acactaccag
    42481 attttgacct tataatgttt ctcgttgatt tagttacatt tgttgaaaca tctttgcgtg
    42541 ttttttgtaa agatttaaaa ggtagagtca tcttcattat accaatttgc actactgctt
    42601 cttttctctt tttatctact gaaactacac taccattttg atttaaagtt attaccttaa
    42661 cttcttcacc tggctttaag tcttttatct ccttatttga tacctttgga actatcatac
    42721 tttttactgt tggctgaaga cttcccatag agctagtcaa ttcttttcta agttcttcta
    42781 tcttacgatt cttttctttt gaagctcttt cttgttcaag acttctaagt tcttttatta
    42841 taatatctac ttcttcttta gcttgtctta taattgaaaa tgcttctgat ttagcttttt
    42901 caatcatctt atctctttga ctgaccaatt tttcaagttt ttcgtcatac tctactttca
    42961 gtttttcaat ctcttctttt aatctttcag cttcttctct atcttctact gctttgattc
    43021 tattcttttc aacattctgt aacacatctt ctaaagctat gttttctgta tttatatatt
    43081 ctttagcacg tgaaatcaca taatcactta gcccaagttt tctagaaatc tcaaacgcat
    43141 ttgattttcc gggaacacct ataagtaact tatatgttgg gcttaaagtt tctatatcaa
    43201 attcaactgc tgcgttttct acacctggtt tagtaagagc ataattttta agctcactat
    43261 agtgagtggt agctatacat ttagctccta cagaatttat atcttctaat actgctattg
    43321 caagggcagc tccttcaaca gggtctgttc ctgctcctaa ctcgtcaaat ataactaaag
    43381 agtctgctgt tacgttttgt aatatagata ctatattcgt catatgagat gaaaatgttg
    43441 atagactctg ttcaatactc tgctcatctc ctatatctgc aaatacatta tcatatacac
    43501 acatactact tccataatca gcaggtatat gaagtccact ttgtgtcata agtgcaaata
    43561 gacctacagt ttttattgta acagtcttac cacctgtgtt tggacctgtt atcaccaatg
    43621 tatggaagtc tcttcctaaa taaatggtat ttgctactac cttcttttta tccagcagtg
    43681 gatgtcttcc attttttatg tttaaatatt tatcctcatt taaagttggt tctattcctc
    43741 tcatctgtat agaaagtttt ccctttgaaa atgcaaagtc caatcttcca agtatctctt
    43801 gattagagat taaatcttcg ctcacttctc caaccatagc agaaagctct gataaaattc
    43861 tttctatttc ttcttgctca cctagtttta actgtctcaa ttcattgttc atctctacta
    43921 tagtcattgg ctctataaaa agtgtcgcac cagaagatga ctggtcatgc actataccag
    43981 ctacttgcga cctatattca gattttacag gtactacaaa tctatcacct cttagagaaa
    44041 ttattgcatc ttgtaagtac ttttgataag tagttgaact tattattgaa tttaattttg
    44101 acctaatgga ttgatttttt tgggctattc ttcttctgat atctctaagt gtacttgaag
    44161 cattgtcaga tatttctact tcactaatta tagcattgta tatctggtct tctatgtcct
    44221 tgtatacata tagagaattt gatagagatt gtattattgg ataattaaaa tcttcttcat
    44281 cactactaga taagctattt cttaatcttc tagccactct taaggtatct gctatcataa
    44341 tcagacttcc tgggtcaaga gaagcaccta tataagctct ctttacctta tcttctatat
    44401 catgaatacc ttccaaccct actgaacctc tctttataat aattgattga gcttcactag
    44461 tttcttctag catagatttc acttctacaa aatcagtatt tggtactaaa ttttctatat
    44521 attttagacc taaagaagat gaggctttct tctttaacaa atctattatt ttattgtatt
    44581 ctagaactct taacgatttc tcattcattt tctatattca ccatcctcga tattttaact
    44641 tttttattta attttaaaaa tgtatgtttt caagcatttc caataaaaac tgcaagtttt
    44701 atttcttttt catttctaaa attattatat tttttctctt ttagatatat tttacacata
    44761 ttttgtatta tactatgtat gtagctatct atatattata cataaacatg ctttatttgt
    44821 aatataatct gttaaaaaat cttctttaat atttttaaag gagtttattt tagcttataa
    44881 aaaaaaatag ctacttatat agataaatta aggaggaaca tgagtcatgg gaaacgtatt
    44941 agaaggatta aaaccagaaa gtgtttttaa aaactttgag aaaatatctc aaattcctag
    45001 aggttctgga aatgaaaaag gcataagtga ttttttactt agctttggta aaaatttagg
    45061 tcttgaaact attcaggatg aaagcttaaa cataataata agaaagcctg ctacaaaagg
    45121 atatgaaaat tgtccaggtg tagttcttca aggacatatg gatatggttt gcgaaaaaga
    45181 aaagaatgtt gaacatgatt ttcttaaaga ccctataaaa ttaagaattg atggtgacat
    45241 gatatatgct actggaacta ctctaggagc tgacaatggt atagctgttg ctatgggtat
    45301 ggctatatta gaagataata ctttagagca tcctgcacta gaagttttag ttactgttaa
    45361 tgaagaagat ggtatgaatg gtgcagatgc actagaccct agcttaatta aaggtcaata
    45421 tatcctaaat atggactccg aggaggaagg atacttatta gttagctgtg ctggtggtaa
    45481 aacttgtgtt gttagtttac ctgttgaata caaggaagtt aaaggtgaca aacaaggatt
    45541 attagttgag gttactggac tattaggtgg acactctggt atggaaattg ttttacaaag
    45601 agcaaatgct aacaaagcaa tagctagagt attaagtgta ttgaatgttg attatgaatt
    45661 agcttctgtt gatggtggta ctaagcacaa tgctatacct cgtgaagcta aatgtgtaat
    45721 agctgttaat aaagctgatg ttgaatctgc taaaaaacaa ataaatgaca ttttaactgc
    45781 atttaaacat gagtttacta cttctgaccc tggcatgact tactcagtag ctgaaacttc
    45841 tgtagacaaa gttcttacta aagattgtaa agaaaaagtt gttcaaatgt cttgcttaac
    45901 tcctcatgga gttcaatcag taagtcttga tatagaagga ttagttgaaa gttctactaa
    45961 cttcgctata atcgaaacta aagaatctac tattgaattt ttaacttctg ttagaagttc
    46021 tgttatgtct attagagatg aaatcgctga tagaataaga cttttggctc aagctttagg
    46081 tgctaattac gaccttatag ctcaataccc agcttgggaa tttaaaaaag gctcaaaatt
    46141 agaaaaaata tgcagtgaaa cttatgagaa attaactggt aaagttccta ctgttatggc
    46201 tctacatgct ggtcttgagt gtggattatt acttgataaa cttcctcatg ctgaagctat
    46261 ctctatagga ccagatatgt ttgatgttca tactccaaat gaacatgtta gtatcccttc
    46321 tgtagctaat gtttgggatt atgttattga aatattaaaa tctatgaacc aatattaaat
    46381 ttaagttttg attctaagta gattttaatt ttaagtaatc tatataaaat taactctgta
    46441 aattaatact acggttagta taatatttac tttcaatatt aacttataga aataaagcct
    46501 aatgatagat aataacatgt atcattaggc tttattgtaa tttaaatatt attaagcttt
    46561 attctgtttt aaaattaatt cttatactat tttaaatcta tttcttcctt gtctcttaga
    46621 atcataaaga gcattatctg ctatttgata cagttcatca tactcttgac caggctttgc
    46681 tataactacc cccatactaa tagataaact ttctccattt ggcaacttaa tgagatgaat
    46741 atgattgtat acatcattac atttattaat aagttcttcc ttatttatta tatcattgat
    46801 ataaacacta aattcatctc cacccaaacg acctactata tcattttttg caaaactttc
    46861 atcaagtgcc tttgcaacat caataagtac tttatctcct aacaagtgac cataagtatc
    46921 attaatcgat ttaaaataat caatatctat aatcaataaa gcaccatttt tttcagttga
    46981 taaattcaac aaattttgtg aagttaattt tctaatatat gctgcattat aaatattggt
    47041 taaactatcc ctttgagcct tatctaacaa tctattcttt tcctcttttt cagaatcaat
    47101 attacatatt ttcccgatta catacattgg tatttttcta tcattataaa ttatttttga
    47161 agtaactcta atccaatgaa aagtatcatc aagcataata gaatacattt cagttgttcc
    47221 attttcttgt gattttatta catcaaaaaa ctttttatta tattctttcc ttattttatc
    47281 tctttcattt tctatattgc cattttcact ttccttatca tttttatcca aaccataata
    47341 agtctcaata cccttttctg gcatagaaat tatcaattta tcttgcttat aatcatactc
    47401 aaaaaagtat tcacttgtca tttcatacaa ctgagtatgt tttttgagtt ctaaagatat
    47461 cttctcgttc atacttgtac gagtatgtaa accaaatgcc ataaatccaa taatacaaat
    47521 taataaactg gctacaataa aaatagtctc aattggattc tgttgcataa tataaagcaa
    47581 cgagaaatct tgtctatatg tagtattttg gtagacaatt gattgcattt tttctgatga
    47641 aatacttata atagctttat taaatatatt caataattct tgtttatctt ttttaattat
    47701 accaaaacaa atactctttg ctttataggt ttgaggaact agacgcacat ttttatactt
    47761 aggttgattc atatagtact gcatagaata accttcgcca taagtgtaat ctgcatagcc
    47821 tttattaaca gcttctatac actcttctaa attatcatac caaactatat tctcttcaaa
    47881 ctgaccataa taattaatac ctttggctag agctacacgt tttccatcta aactattctc
    47941 atttaattta ttattaatca tcatagtata ttgagtagaa agaaaaggcc gagacatagc
    48001 tatattatgt tcacgagagt attcataatc ataaatcatt cctgctacaa tatcaatttc
    48061 acgcttttta gtcatgtttt ctaaatcttc ttgagatttt acaggcacaa gttcaaattc
    48121 taagcctgtt ttatcacata tatgatttaa caaatcaatt gatatacctc ttaattcacc
    48181 tgtatcttta tctatatttt gaaatggtgg tttgtttaaa agtactccta cttttaattt
    48241 ttctgtctct ttaatgtaat tagtttcttc atcagataaa aacatttgat tattctctgg
    48301 tgtaaagtat ttttcatata aagtagttga aaaatatggg tctttctgtt caatattatt
    48361 aattgctgag ttaagctttt gagttaaatt tgtactacct ttaggagtaa tgaaataaaa
    48421 tggttttgat gaaaattttg ctacagtacg tacaccttct attgggttca tactcacatt
    48481 aagaagtaca tctgctttac cactctttag tgcttctagt tgctcatcat ttgttttaca
    48541 atataccaat tcaggtgaca taagattcat tttacaatac tcatctaatt ctttaagtct
    48601 agtatcagaa ttttctatta cagcaatacg gatttttttc acatcttgtg aactcattat
    48661 gttatcaata tctgtatttt catataatgc ttgtagtact gtatttgcta ctccataatt
    48721 attccctgca taattatata ttttttgcat tggttcattg tataacatac ctcccattat
    48781 atcaatttca ccattttcta acatcttcat taattttgta atagattcat ttatctctcc
    48841 aggaacttga acaaattcat aattccatcc agtatactgt gcaatttcta gtaaatattc
    48901 atatgtatac ccaaaatagt taccttgctc atctatttca gttaaacctt tttgaatagg
    48961 ataagcaaca cgaatggtac gattatcatt acctatagcg ttgataccaa aagaaaatat
    49021 gcaagaagtg aatatagatg taattacaat aattaataaa agtaattttt ttaaattcac
    49081 tacacaatcc tccatatagt taagatttac agtataaatt actgcataaa cttgtagaca
    49141 tgtaaaaata gattagtata taaaaggata tataaatcta cttagaaatt taaatatcct
    49201 taatatatac aatatattta aattataact catttatatg tctattacaa caaatttacc
    49261 ctgctaaata cctactctat atcccataat tttacaagtt gtagtgaagt ttaagttcta
    49321 tctcacctca aatttagcag tgtttctatt tacagttctt ttatatttca catctggata
    49381 acccaatata aatgaagtga taactgcttc gttctcgctc attcctaaca gttcttttat
    49441 ctttggctct acatttgatg ccataacaaa aaatccatta taacaaattc ctaatccttg
    49501 agaataggcc ataagttcca tattagatgc agccaatcct ccatctacat aagcacttcc
    49561 acctagagac atatcaccta caactaccat aagtagtggg gcatcataaa ataaagaatc
    49621 atttccgttc tctttgtatc ttttgtacat tttcttaaat gtatttttat agatagaacg
    49681 tacagggtca ttgtcatcag tatctaatgc caaattataa agaccttgta ttgccagttc
    49741 tttcacttct tttagctttt cttttactaa tatgtatctt ataggctgtc tattaccacc
    49801 tgttggtgtg tatctaccag cttccactat gttttttatt ttttcttctt ctacctctat
    49861 atctttatat tgtcttatac ttcttctaaa tttaataaaa ttcaataact tttcgggttc
    49921 taattcaaaa ctttcacgat tgtattctat tacttcattt ttatcaaatc cttgcatttc
    49981 tattgcatct gttgggcaaa ttgccataca gtgaccacag ttaatacaga gaatattttt
    50041 aacctttgct ttatcatcct taacttttat tgcatttaca agacagtctg attcacacat
    50101 cccacagcct acacattttt ctaaatttat gtctatcatt ccaaattatt cctcctttta
    50161 aaaaatttac tttagtataa aacaaatagt tgtctaagtc aatttttatt tttttaacaa
    50221 tatactagcc ttaactacac tttaatggag aaaaatatac ataaattgga acttaaatta
    50281 gaacttattc aaagtaaaag ataaaagaag tagatgtcta aaagtgaact ttttagttaa
    50341 ttagatattc tttattgtat gaaatcaagt ggatatttat catttcaaca ataaaaaagg
    50401 cttacctcta ttttggatat gtccctgtca agtagacaga ctaaaaaaag gctatcttta
    50461 tgcactgaga tagccccttt tcccaactac tattgtttat cttaatatta gtcaataaat
    50521 ttaatttgct ataacctgta agttacattt taatttgatg taactttaaa ttacattttt
    50581 aagtaatatc tttaaattat attcttaaaa tatatcatta aactacattt taattaatct
    50641 aaaaactata tcttaattaa tctaagaaac ttacattata atcttttagt ttcccattat
    50701 catatacaat ctttgcacta aagatatttc cagtcttagc ccaatttaag aacagcctgt
    50761 caccttccca catatttaaa tcgaatattt tatcattatc aacccaaatt aaatttcctt
    50821 ctttacaatc atttaaatct ttttcagata attcaccttc aaactcatct gagaaataca
    50881 aaaacatata ccaatcttct tcatctttaa attttggaaa agttatcaac cctttatatg
    50941 acaatgattt aagagtaaga ccagtctctt cttttacttc tctaattaca cattcttctg
    51001 gagtctctcc ttgctcaaac tttcctccaa ctcctacata cttaccttca tgtatatcat
    51061 ctttcttttt atttctgtac aacatcaaag tcttgtcatc tttttctata taacaaatag
    51121 ttgtaagttt catgtatctc gccttgcttt ctcttttata ttctcaacta attattttca
    51181 gcactttcaa gcacatcact tatattcttc attgcattaa gagaaagctc tgcaaattca
    51241 tcaaatggaa tccctatatc ttctatactt ctcattgcat ctctatttac atttgaagca
    51301 aatctcgttt ctttcattct tttaataaca gacttagtct ttacactggt aagtttttta
    51361 tctggataaa ctaaagctat agcagttata aacccagtta tagggtctgc tgcatatatt
    51421 gctttttcca ttaggctagt tcttgtaact cccattccat catgatgacc aagtattgct
    51481 ctatacattt cttcgttacc gaagttttct tttttaagca gttctactgt tgttgtagca
    51541 tgtccttcac atatttctcc tggctttctt ccaactatat ctgaatccaa atcatgtaaa
    51601 agacctgcca ctgcccactc ttcttctttt tctggctcta gtcttttggc taattctctc
    51661 ataacagctt caacagcata acaatgtttt ctcattctat cagtttctaa atacttaaat
    51721 aaaatttcta aagcttcttc tctcattttc accaatcccc ctgttttttc acatatatga
    51781 tatttttata tattaataca cataaattca agcttttata tatattcatg tatatttttc
    51841 atgttatatc tattctacac ctttatagta gtgtcctgca actgttccaa tttcatctaa
    51901 tttcttatac aagtctgtag ccttttttcc aagtttataa ttattactca gtacctcaat
    51961 gtgagcttca ataacctttt taaccatctc actatcttca actgtaaaat ctatcctaaa
    52021 tacatctatt ccaatttcct ctaactcata tagattgtcc aacatacaag taaccttcga
    52081 attgtaaatt gtacttctac agaatatatc ttgtgttatc ctataacttg catccgtaaa
    52141 gtctcttaaa gcataactac tttccttaca tttagcaact ctcttgtcct ttttacaatc
    52201 tctaacaact actcccatag gacagtattc tgtaaccatc ataggagcat atccatatat
    52261 tactgcttca atttctacat ctgtatattt taaagtttgt ttaatttcat ttaagtttag
    52321 ttcttgtgac aaacaaactg tacttgctcc ctcttctttg aagtgattta tactttcact
    52381 attgaataca tttaagtagc aatctatatt taagtctttt ttcttaaagt attccataga
    52441 gccccagtta ccaatttgaa ccttattaat tccttttata ttatccaata tctcaaattg
    52501 cttgtactct ttatttctaa caatccttgg tgctgaatag attaattttt tgtcagtgac
    52561 tgctgtatta gagtggttct tattcaaagt atctatttta cttgtatttg acaaagtatc
    52621 tatgttattt gtatttgaac ccattagttc tttaggagta cctaaagacg aagaagtatc
    52681 taaatccata gaaatacttg aatctataga aatatttgaa tctatagaga tacttgagac
    52741 catttgtaaa gcttttgata atgtatttat atcttcataa tagattaaat ctacattata
    52801 atttaatact gcttctaatt gctccaagtt cttaacttta actctaatct taggagctgt
    52861 atcaagtttt ttctgtttat ttactctatt tggctggtat tttaatctat tcttcttaaa
    52921 ttttctatct tttatcttta ttctttcttc atctaaaagc tcaatacaat cccttctcat
    52981 ttgatttaac acacttatag gcatacttac accttcatcc aggtcaattt ctatctcttt
    53041 taacttatat ggtgtatttc caagcttttg aatttgtgtt tctatttttt ctttacttaa
    53101 agccaccttt atagcttttt caactatttt gtctccttct accaccgatt tatttccaca
    53161 aaaatcactt ataaaaagct ctggcttttt acctagttta atactcactt ttgcacgtat
    53221 attagttttt atgaactctt tatcttgctg aaatgtagct tgtactctat ccattaattc
    53281 gctgtcagaa gtcttatata taagctgatt tctctttgct tctccaacaa aatctaattc
    53341 tatcaaatcc cctttatgtg ctattgtagt tatctctcca tcctttatta ttcttccaat
    53401 agtacctcca cctaagttaa ttccatcacc tttcttaagc gtattttcta gcattactct
    53461 aagtcttttg ttctttctat tataatctaa aacttttcca atatatagcc cttgattatt
    53521 aggtctatta gagttcatta tatccttacc aacttcacct aatatatatc cttttgtaaa
    53581 ctttctatta aatatggtat acaaattatt tattatttcc tcagaaatac ttattttttt
    53641 cttatctaca aattcattaa tcgcttctct atatcctgaa accacagtag ctacatactc
    53701 tggctttttc attctacctt caatttttaa agataataca cctgcttcaa ttattttatc
    53761 caaatcttct atagtactta aatctctagg actcaacaaa taatctccat tactttttac
    53821 tacttctcct gtatttatat ctatcaactc atacttttgt ctacatggct gtgcacacct
    53881 acctctattt ccagacctat tgcctatcat actactcata aggcattgac ccgagtaaca
    53941 tacacataaa gctccatgta caaaaacttc tatatctaca gttgtatttt cacaaatcca
    54001 ttttatctca tcaacattta attctcttgc taaaacaact ctatcaaacc ctacactctg
    54061 aaggtatttt acatcttcta aggaatgtgc taccatttga gtgcttgcat gtagttcaaa
    54121 gtcaggtaat agttttttta taagtcttgc cattcctata tcctgtacta ttaaagcatc
    54181 aatatctata tcgtataaaa attttacata ctctacaaaa tcttctattt catactgttt
    54241 tatcaatgta tttacagtaa caaagacttg cactcccctt atatgagagt atttaacagc
    54301 ctcaatcagc tcatttctat caaagttatt tgcagatgct ctagcactaa agtctttacc
    54361 acctaaatac acagcatttg ctccgttttg aactgctgct tttaatgaat caaaagagcc
    54421 tactggtgca agtaattcta tttttttcat atttttctcc tctatttata cttgttaggt
    54481 taatataatt atatctataa cttaaatatt tgtattaaat ttatatattt cttactatat
    54541 tttaaattct tcaatcaatt tatatattag ttatttatat ttggttcatt tgagttaaca
    54601 tatacattat ttactatatt tagtatattt ctattaactt atatattgat tactgtattt
    54661 taaattcttc aattaattta tatactgctt actaaattta aaacatttta attaatttgt
    54721 acactgttta ctatacttga aatacttcta ttaatctata tattgcctac tatacttaag
    54781 acattttaat taatttatac actgtttact atacttgaaa tacttctatt aatctatata
    54841 ttgcctacta tacttaagac attttaatta atttatacac tgcttactat acttaaaata
    54901 cttctattaa cttatacatt gcttactata ttttaaattc tttaattaat ctatgcaata
    54961 tttgctatat ataaagtata tcaaataaca gcttcatttt tttgtaaatt tacagaaagt
    55021 taattttcat taaatgaatc atctttattg ataaacataa ttccaaagac cactgccaaa
    55081 ataaaaaaac atattagtat tgatttttcc acgaaaattt ttgtaaaaaa agttcctaaa
    55141 atagctccag atataaagaa tataataaca gcataatact gtaaactctt ttttatcata
    55201 aaaatattct tagtatgtat ataatgaaat aaagtttctg tagcacttct taaatttcca
    55261 gtacacattg tcgttgcaaa actattccca tttacttttc taaaactctc tacttgcatt
    55321 gaacaaataa aagaaatagc aacatttaca aacatattaa atcttcctct aggaataaat
    55381 gaaacaataa gtattgtaat catttcaatt ataatgataa cctgacgcca atgaatcttt
    55441 gtcttattat ttgtaaaata ctttcttatc ttctcggcta taaatacacc taacataaat
    55501 gccaaaatag gaatcaaata ataaaatgat tcctttacat taccttctgc tatattaagc
    55561 ccaaacaaaa ctatatttcc agtttgtgca ttagcaaaga ctttacctct taccacataa
    55621 gtataaatat ccaaaaatcc tcctgttatt gccaacaaag aacataaaaa gaatgattca
    55681 gacatctgac tcttgcttgt catatataaa cctctcgctt tctaatgata gatacaaaat
    55741 caaaatcatt ttagattcta tttttcattg atataaaaaa attatttata tttaaatatt
    55801 gtctatattt atacatcatt tatatttata cattaaaaga agctgtctca aaaaacaaga
    55861 gacagcaaat cttttagttt attccacatt ttttgcatca ttatttaacc ctgtacattt
    55921 taactgtaaa tcataagctt tgttttgaaa ctcagaagct aagttttcag ctatagtagc
    55981 tctttcattt gcttcttcaa gttgagtact taactcctca atcttgtttt tataagcatc
    56041 taactctgct ctagaacctt ctgtcgtatt actaagttcg aatatttctt gctttttatt
    56101 ttctataatc tcaattaact ctttcatctt agtttcagct tcacgatttt ctgcttcttt
    56161 ttctgctatc gtaagcttta aagacttgat ttctaattga agctcttcat tagatgctcc
    56221 aaccttctta ctcaattctt cattagcttt aatcaacttc tcattctcat ctgaacattc
    56281 aaataaaagg tctgatatat ttaatgcagt taatatcgca gcaactgata agctcaattt
    56341 cgggttttga cgtgttattt tatccatttc tttgtctaca aaatcagcaa tactaatcat
    56401 aaacttctct gatttgtctc caaccattgg atattcagcg ccatggattt taaccattac
    56461 tttgttcatc ctaacctccc ccgacattag ttgttaattt gatcttaagt tagcatttaa
    56521 tttttcgctt aattcactta atatcttatc atgcacctta gctacatctt catctgttag
    56581 agttttatct ttacttctat aagttattga atatgcaatt gacttgtatc cagcttctat
    56641 ttgagaacct ttgtaaacat caaataactt gtagctttct actaatcctt gaccattttc
    56701 ctttattata tcttctattt gttttacaaa tacatcatct ttaacaataa gagctatatc
    56761 tcttgatgta gatggatatt ttggtaatgg cacataattc tttgttctat cagaattatc
    56821 aaatacaaag tcaatatcta tttcagcgac ataaactctt tgacctagat tgtagttttc
    56881 tataacatct ggatgcaatt cacctaatgt acctacatat ttattattat acactatttt
    56941 agcacatcta ccagggtgga aggtagtatt attatcttga ggttcaattt cataaccttt
    57001 aaatccgaca tttacaagta tagtttctat agttcctttt agtgcaaaga agtcaacatc
    57061 tttaccatac atacctacac acattctatt ttcttcctta ggtaattctg aatcttgtgg
    57121 gatgaatata tgtccacact caaatgcaga tacttcttct attttatgag atacattagt
    57181 tgataaaaca tctaacatat ttggtattaa agtagttctc attacagaag tttcttctcc
    57241 taatgggtta agtaatttaa caaaatttct ctttgcatta ccttctggta ctcttatctt
    57301 atctactccc ttagggctta caaatgaata tgttaatatt tcatttaatc ctacagcagt
    57361 tgaattatct tttaaagcat ccatgaattt ttgttttgaa gttttaattc ctgctgtcgt
    57421 atttccttct aattgtacag aaggtatatt ctcaaatcca taaattctag ctatttcttc
    57481 ccatacatca gcttcttgtt ccatatctgt tctaaagctt ggtacatcta tttctaattt
    57541 atcattagct accaaattac atttaaactc taatgattct aaaattccta caaactgctc
    57601 cattggtaca tctacaccta atagatggtt aattctttga ggatttacta ccaatttttt
    57661 aggttctggt ttatttggat atacatctac aacacctttt aaaacttttc ctgctcctaa
    57721 catttcaaca agctgtgcag ctctatttgc tgctatctct gcaagattag ggtctaagtc
    57781 tttttcattt cttgaagatg cttctgacct aataccaact tttttagctg tcattcttat
    57841 gttttctggt ttgaaattgg cactttcaaa aagtacagtc ttcgtattag aagttatttc
    57901 tgagttagca ccacccatta caccagctaa accaagtgat ttttctccat ttgttataac
    57961 tagcatatct ttgtctaatg ttctctcaac atcatctaat gttacaagtt tctctccatc
    58021 tttagcattt cttactacta ttcttccagt ctctacttga tttatatcaa aagcatgaag
    58081 tggttgacca agctctaaca ttacgaagtt tgttatatcg actatgttac ttataggtct
    58141 tactcctgct tctgtaagtc ttctttgcat ccaatatgga gaaggttcta tttttacatc
    58201 tgtaaccatt ctagcaacat atcttctaca taaatctgga ttatctatct caactttgaa
    58261 atctatctct tcgtcacttt cttttacttc gatttcagga tattttactt ttgttcctat
    58321 agttgcagct gcttctctag ctatacccat catgcattta caatcaggtc tgtttgaagt
    58381 taattcaaaa tctattaaag catcttttaa tcctaaaaca tcttttatat cttttcctaa
    58441 ttcataagaa tcttcgtggt ctaaaatata tataccacct cttttatact cctcaatgta
    58501 atgttcatct atacctagtt cagctgctga acacatcata ccatctgata attcacctct
    58561 gaaatcagtc tgtttgattt caactcctcc aggtaactta gaaccattta cagctactgg
    58621 aatataatct ccttctgata tatttgtagc tcctgtaact atttgaacaa ctttatctcc
    58681 aatatctact ttggttacaa ccaacttatc agcatttgga tgttgtttta tttctaaaat
    58741 ctttccaacc aatatatttt ctatttcttc accataataa tctattgttt caactttagt
    58801 tcctgtcatt gtcattttat cagcgaactc ttttacatcc atgtctatat caacatagtc
    58861 tctaagccat tttaaagata ctaacatcta cataccctcc taattaaaat tgatttaaga
    58921 atctcatatc attttcgaat aataatctaa tatcatctat ttcgtattta agcattgcaa
    58981 gtctttcaac cccaactcca aatgcaaatc cactgtaaac ttctgggtct attccacaat
    59041 ttctaagcac atttggatga accatacctg aacctaatat ttctatccaa ccttcatatt
    59101 tacacattgg gcaaccttta ccaccacatt tgaaacaagt aacatcaacc tctgcacttg
    59161 gttctgtaaa tgggaagttg tgaggtctaa acttagtttt gatatcagaa ccaaacaatt
    59221 tttcaacgaa gatatccata gttcctttaa attctgccat agtaacatct tttccaacaa
    59281 caagcccttc tatctgatgg aacattggtg agtgtgtagc atctggcgag tctgacctaa
    59341 aacatctacc tggtgcaatt acttttattg gtaactcttg acttctcata gttcttactt
    59401 gaactggaga tgtttgagtt ctaagtaata ccccatcatt gatatagaat gtatcgctca
    59461 tatctcttga tggatggtct ttaggagcgt ttaatgcgtc aaagttgttt tcaacagtct
    59521 caacttctgg cccttctgct atagagaatc ccattccgat aaatatatct gttacttcat
    59581 ctataatttg agttattgga tgcttcttcc caacctttaa aacttttcct ggttgagtaa
    59641 catctatcac ttcctctttt aattttcttt ctttttcaat agcatttatt tcttcttttt
    59701 tagaatttat gctaagttca atgttttctc ttacctcatt ggcaacctta ccaactactg
    59761 gtctttcttc agcagataat ttacccattt ctttaagtat ggcagttatc tcaccttttt
    59821 ttcctaagta cttaactctt aaactttcta cactttctat gctttgtgct tcttttattt
    59881 cagccaaagc tgcttcacgt aaagcaagta atttttcttg cacacatatc accctttcgt
    59941 ttttttgtat aaaaaaagcc cctcaatccc tgtctaggga cgaaaggcta tagtttcgcg
    60001 gtaccaccct agtagtttaa caatttaaaa atcatcaaac cacctctaac aaacttaacg
    60061 cgtttttgac gtcaaagcct actgtaaaat ttcagctctg ttactccaga gggaacttct
    60121 catgacctct tagaaaacac tcccagccta ggtgtttatc tctttactaa gtttaatcaa
    60181 aatactttcc tctttcattg catttacaat attatattgt ttattttagt attatatcat
    60241 ttccattcaa attatgtgac tatttttaat atatatccaa tacataatta
//
LOCUS       DBGJFU010000007        59465 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.007, whole genome shotgun sequence.
ACCESSION   DBGJFU010000007 DBGJFU010000000
VERSION     DBGJFU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.007"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            172..417
                     /locus_tag="ACOIAS_000428"
     CDS             172..417
                     /locus_tag="ACOIAS_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HGZ2700159.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            452..1177
                     /locus_tag="ACOIAS_000429"
     CDS             452..1177
                     /locus_tag="ACOIAS_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGZ2700160.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            1199..2512
                     /locus_tag="ACOIAS_000430"
     CDS             1199..2512
                     /locus_tag="ACOIAS_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2700161.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYDENLKKIV"
     gene            2718..3257
                     /locus_tag="ACOIAS_000431"
     CDS             2718..3257
                     /locus_tag="ACOIAS_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGZ2700162.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            3346..5331
                     /locus_tag="ACOIAS_000432"
     CDS             3346..5331
                     /locus_tag="ACOIAS_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HGZ2700163.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            5555..6523
                     /gene="spoIIIAA"
                     /locus_tag="ACOIAS_000433"
     CDS             5555..6523
                     /gene="spoIIIAA"
                     /locus_tag="ACOIAS_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HGZ2700164.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            6513..7034
                     /locus_tag="ACOIAS_000434"
     CDS             6513..7034
                     /locus_tag="ACOIAS_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HGZ2700165.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            7051..7248
                     /gene="spoIIIAC"
                     /locus_tag="ACOIAS_000435"
     CDS             7051..7248
                     /gene="spoIIIAC"
                     /locus_tag="ACOIAS_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HGZ2700166.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            7273..7650
                     /gene="spoIIIAD"
                     /locus_tag="ACOIAS_000436"
     CDS             7273..7650
                     /gene="spoIIIAD"
                     /locus_tag="ACOIAS_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HGZ2700167.1"
                     /translation="MQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVMFKL
                     NFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEFGGK
                     IIVMSMSFPILLSIVEMVVNIIP"
     gene            7735..8940
                     /gene="spoIIIAE"
                     /locus_tag="ACOIAS_000437"
     CDS             7735..8940
                     /gene="spoIIIAE"
                     /locus_tag="ACOIAS_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HGZ2700168.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            9000..9617
                     /locus_tag="ACOIAS_000438"
     CDS             9000..9617
                     /locus_tag="ACOIAS_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HGZ2700169.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            9629..10183
                     /locus_tag="ACOIAS_000439"
     CDS             9629..10183
                     /locus_tag="ACOIAS_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HGZ2700170.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            10210..10899
                     /locus_tag="ACOIAS_000440"
     CDS             10210..10899
                     /locus_tag="ACOIAS_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HGZ2700171.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSYSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            11009..11371
                     /locus_tag="ACOIAS_000441"
     CDS             11009..11371
                     /locus_tag="ACOIAS_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGZ2700172.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            11456..11962
                     /gene="nusB"
                     /locus_tag="ACOIAS_000442"
     CDS             11456..11962
                     /gene="nusB"
                     /locus_tag="ACOIAS_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGZ2700173.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            11946..13016
                     /locus_tag="ACOIAS_000443"
     CDS             11946..13016
                     /locus_tag="ACOIAS_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HGZ2700174.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            13013..14218
                     /gene="xseA"
                     /locus_tag="ACOIAS_000444"
     CDS             13013..14218
                     /gene="xseA"
                     /locus_tag="ACOIAS_000444"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGZ2700175.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            14220..14435
                     /gene="xseB"
                     /locus_tag="ACOIAS_000445"
     CDS             14220..14435
                     /gene="xseB"
                     /locus_tag="ACOIAS_000445"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGZ2700176.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            14438..15325
                     /locus_tag="ACOIAS_000446"
     CDS             14438..15325
                     /locus_tag="ACOIAS_000446"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HGZ2700177.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            15355..15792
                     /locus_tag="ACOIAS_000447"
     CDS             15355..15792
                     /locus_tag="ACOIAS_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HGZ2700178.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            15826..17691
                     /gene="dxs"
                     /locus_tag="ACOIAS_000448"
     CDS             15826..17691
                     /gene="dxs"
                     /locus_tag="ACOIAS_000448"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGZ2700179.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            17824..18636
                     /locus_tag="ACOIAS_000449"
     CDS             17824..18636
                     /locus_tag="ACOIAS_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HGZ2700180.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            18656..20335
                     /gene="recN"
                     /locus_tag="ACOIAS_000450"
     CDS             18656..20335
                     /gene="recN"
                     /locus_tag="ACOIAS_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGZ2700181.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            20550..21416
                     /locus_tag="ACOIAS_000451"
     CDS             20550..21416
                     /locus_tag="ACOIAS_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700182.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(21509..21988)
                     /locus_tag="ACOIAS_000452"
     CDS             complement(21509..21988)
                     /locus_tag="ACOIAS_000452"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2700183.1"
                     /translation="MAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQDFNNE
                     LKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKIGKIL
                     LEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYMELKL"
     gene            complement(22061..22501)
                     /locus_tag="ACOIAS_000453"
     CDS             complement(22061..22501)
                     /locus_tag="ACOIAS_000453"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2700184.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            22662..23720
                     /locus_tag="ACOIAS_000454"
     CDS             22662..23720
                     /locus_tag="ACOIAS_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HGZ2700185.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGGAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            23862..24686
                     /gene="spo0A"
                     /locus_tag="ACOIAS_000455"
     CDS             23862..24686
                     /gene="spo0A"
                     /locus_tag="ACOIAS_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HGZ2700186.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            25099..26220
                     /gene="steA"
                     /locus_tag="ACOIAS_000456"
     CDS             25099..26220
                     /gene="steA"
                     /locus_tag="ACOIAS_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HGZ2700187.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            26238..27113
                     /locus_tag="ACOIAS_000457"
     CDS             26238..27113
                     /locus_tag="ACOIAS_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HGZ2700188.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            27137..27829
                     /locus_tag="ACOIAS_000458"
     CDS             27137..27829
                     /locus_tag="ACOIAS_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2700189.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            27837..28661
                     /locus_tag="ACOIAS_000459"
     CDS             27837..28661
                     /locus_tag="ACOIAS_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HGZ2700190.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            28666..29733
                     /locus_tag="ACOIAS_000460"
     CDS             28666..29733
                     /locus_tag="ACOIAS_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HGZ2700191.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            29735..30271
                     /locus_tag="ACOIAS_000461"
     CDS             29735..30271
                     /locus_tag="ACOIAS_000461"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGZ2700192.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            30339..30944
                     /locus_tag="ACOIAS_000462"
     CDS             30339..30944
                     /locus_tag="ACOIAS_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HGZ2700193.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            30956..31870
                     /locus_tag="ACOIAS_000463"
     CDS             30956..31870
                     /locus_tag="ACOIAS_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2700194.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            31912..33084
                     /locus_tag="ACOIAS_000464"
     CDS             31912..33084
                     /locus_tag="ACOIAS_000464"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGZ2700195.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            33131..33943
                     /locus_tag="ACOIAS_000465"
     CDS             33131..33943
                     /locus_tag="ACOIAS_000465"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGZ2700196.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            34147..35472
                     /locus_tag="ACOIAS_000466"
     CDS             34147..35472
                     /locus_tag="ACOIAS_000466"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HGZ2700197.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            35685..36764
                     /gene="mltG"
                     /locus_tag="ACOIAS_000467"
     CDS             35685..36764
                     /gene="mltG"
                     /locus_tag="ACOIAS_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HGZ2700198.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            36936..37610
                     /locus_tag="ACOIAS_000468"
     CDS             36936..37610
                     /locus_tag="ACOIAS_000468"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HGZ2700199.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            37597..38844
                     /locus_tag="ACOIAS_000469"
     CDS             37597..38844
                     /locus_tag="ACOIAS_000469"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGZ2700200.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            38916..40580
                     /locus_tag="ACOIAS_000470"
     CDS             38916..40580
                     /locus_tag="ACOIAS_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HGZ2700201.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(40677..41318)
                     /gene="sigK"
                     /locus_tag="ACOIAS_000471"
     CDS             complement(40677..41318)
                     /gene="sigK"
                     /locus_tag="ACOIAS_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HGZ2700202.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            41885..42247
                     /locus_tag="ACOIAS_000472"
     CDS             41885..42247
                     /locus_tag="ACOIAS_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700203.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            42231..42938
                     /locus_tag="ACOIAS_000473"
     CDS             42231..42938
                     /locus_tag="ACOIAS_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700204.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEIINQFKNESENIRNQEVVEFTEEDEEELKQNIIENIK
                     KNFDEKNLVQVYKYRGV"
     gene            42958..43377
                     /locus_tag="ACOIAS_000474"
     CDS             42958..43377
                     /locus_tag="ACOIAS_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700205.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEVYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            43367..43891
                     /locus_tag="ACOIAS_000475"
     CDS             43367..43891
                     /locus_tag="ACOIAS_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700206.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKCSQKYE
                     TDFKIKVWNFSESI"
     gene            44203..44760
                     /gene="efp"
                     /locus_tag="ACOIAS_000476"
     CDS             44203..44760
                     /gene="efp"
                     /locus_tag="ACOIAS_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGZ2700207.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            44852..45307
                     /locus_tag="ACOIAS_000477"
     CDS             44852..45307
                     /locus_tag="ACOIAS_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HGZ2700208.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            45470..46180
                     /gene="rnc"
                     /locus_tag="ACOIAS_000478"
     CDS             45470..46180
                     /gene="rnc"
                     /locus_tag="ACOIAS_000478"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGZ2700209.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            46234..47304
                     /locus_tag="ACOIAS_000479"
     CDS             46234..47304
                     /locus_tag="ACOIAS_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HGZ2700210.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            47332..50886
                     /gene="smc"
                     /locus_tag="ACOIAS_000480"
     CDS             47332..50886
                     /gene="smc"
                     /locus_tag="ACOIAS_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HGZ2700211.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            50913..52193
                     /gene="ftsY"
                     /locus_tag="ACOIAS_000481"
     CDS             50913..52193
                     /gene="ftsY"
                     /locus_tag="ACOIAS_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGZ2700212.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPVPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            52296..52664
                     /gene="ylxM"
                     /locus_tag="ACOIAS_000482"
     CDS             52296..52664
                     /gene="ylxM"
                     /locus_tag="ACOIAS_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HGZ2700213.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            52661..54013
                     /gene="ffh"
                     /locus_tag="ACOIAS_000483"
     CDS             52661..54013
                     /gene="ffh"
                     /locus_tag="ACOIAS_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGZ2700214.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            54051..54323
                     /gene="rpsP"
                     /locus_tag="ACOIAS_000484"
     CDS             54051..54323
                     /gene="rpsP"
                     /locus_tag="ACOIAS_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGZ2700215.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            54388..54615
                     /locus_tag="ACOIAS_000485"
     CDS             54388..54615
                     /locus_tag="ACOIAS_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HGZ2700216.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            54723..55238
                     /gene="rimM"
                     /locus_tag="ACOIAS_000486"
     CDS             54723..55238
                     /gene="rimM"
                     /locus_tag="ACOIAS_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGZ2700217.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            55247..55942
                     /gene="trmD"
                     /locus_tag="ACOIAS_000487"
     CDS             55247..55942
                     /gene="trmD"
                     /locus_tag="ACOIAS_000487"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGZ2700218.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            56127..56477
                     /gene="rplS"
                     /locus_tag="ACOIAS_000488"
     CDS             56127..56477
                     /gene="rplS"
                     /locus_tag="ACOIAS_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGZ2700219.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            56798..57694
                     /gene="ylqF"
                     /locus_tag="ACOIAS_000489"
     CDS             56798..57694
                     /gene="ylqF"
                     /locus_tag="ACOIAS_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HGZ2700220.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            58083..59375
                     /gene="brnQ"
                     /locus_tag="ACOIAS_000490"
     CDS             58083..59375
                     /gene="brnQ"
                     /locus_tag="ACOIAS_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGZ2700221.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT        24444 a         5864 c        10927 g        18230 t
ORIGIN      
        1 attacctatt tttatttgta agaaaagtgt acatagaatt agaagtaaaa aatacaacta
       61 ataaaactat tgaaaatact agataaataa taaataaaaa taattataga ttttggcata
      121 catattgcta attatagaga taagacgaca aaataattgg gaggtttaat gatgaaatac
      181 aaaacaacag ggacttgtgc tacagaaatt gaatttgaag taaaagaaaa taaagttaca
      241 aatgtaaact ttataggtgg atgtgatgga aatttaaaag ggctaaaagt attagttgaa
      301 ggcatgaata tagaagatgt aatacaaaag ttaaaaggaa tagagtgtaa aacaaagcca
      361 acatcttgcc cagaccaact gtctctagca ttggaaaatt atatgaatat aaagtaatac
      421 ataaaacaat cgattagtta ggagtttaat tatgaaacca ataataggga ttttaggaaa
      481 tttaataata atggaaaatg gtatgtttcc aggtcttgaa aggtcatatg taaataatga
      541 ttatataaat gcagtgctaa aaggaggagg tagtccagta ataattcctg taaatacaga
      601 taaagaagtt ataaaaaaac agattgaaat ggttgatgga gtcttaatat ctgggggatg
      661 ggatatcaat cctcaactgt atggagaaga aacaagagaa gaaacgacat ttatatatcc
      721 agaagtggat gaatttgatt taattgcaat aagtatagca ttagaactta aaaagcctat
      781 attaggtgta tgtaggggac tacaaatttt aaatgtaagt ttaggaggaa ctttatatca
      841 agataataat cttatagaag gaagttatat aaaacataca caatcttcta aaagacatgt
      901 agctactcat aaagttgatg ttaaagaagg aagtatttta gagggaattt taggaaaaca
      961 attacttaca aatagttatc atcatcaaag tgtcaatcag cttggaaaag gattaaaagc
     1021 catagcatac tcaaaagatg gtataataga agctattgag aaagaggatg aaaattttgt
     1081 agtaggagta cagtggcatc cagaaatgat ggtagattac tgtgataaaa tggaaaaact
     1141 atttaaatat tttataaata tttgttcaaa tttataatat accttggggg gattgaacat
     1201 gaacgaaaag aacaaaatgg gattaataag tattatactt ttaggtatta atgcagtagt
     1261 aggagctgga gtttttctat taccaggaga tgcaatgaaa tcatttggtg tagcaagtat
     1321 atttgtatat atatttgata tgttgttagt tttatctatg gcattctgtt ttgctgaggt
     1381 tgcaggtaaa ttcaataaaa atggtgctgc ctatgtatat acaaaagaag cttttggaga
     1441 tttttgtggt tttgaagtag gtcttatgaa atgggtaata ggatgtattt cttggggagc
     1501 tttaatagta ggttttccta catcattatc agcagtatgg gctccagcag gtgaaccaca
     1561 tatacaaaaa attataattg tagtcatgat agttgggctt acaattataa acttattagg
     1621 tgtatctcta tcaaaaattg tacaaaatgt tataactgta ggtaaactaa ttccattaat
     1681 actatttata ggaataggta tattttttat aaaaggagtt aattttactt cttcaacaat
     1741 ggtacctcca ggagctggag caacagagtt tggagcagca gcacttttaa tgttttattc
     1801 atttacagga tttgaatcta tagcagttgc agcagaagat atggagaatc cacaaaaaaa
     1861 tataccaata gcaattataa gtgtaatagt aattgcatca ataatatata tattaaatca
     1921 agttgtatgt gttggtatac taggagattc gttatctagt acatctacac cagtggcaga
     1981 tgctgcaaga atatgctttg gaaatatggg agctggattg gttacctttg gaaccttggt
     2041 ttctgttggt ggaatttgta tgtgtggtgc ttttgtaaat ccaagaagct gtgtagcttt
     2101 agctgatgat aaaatgttac cgagaatatt tgcaagaaaa gataaaaaag gtacaccata
     2161 tgtagcgata atagctacta tgctaatcac tataccaata gctctatctg gaagttttgc
     2221 tgaattagca gcaataagtg cagttgctag attcatacaa tacataccaa catctttatc
     2281 cgtattagtg tttagaaaga aaagaccaga attagtaggt acttttaaaa ctccttttgg
     2341 agcagtgatt cctctaattg ctgtatgtgt tggaacatgg ttattattcc aagcatctat
     2401 gcatcaatta attatgggat taggagcttt ggcaattgga gtaccattat actttataat
     2461 gaagtcttat aatagaaaag tctatgatga aaatttaaag aaaatagttt aattatgtta
     2521 taacaattta ttgagttaaa attagacatt ggcataaaat taaatacaaa taagtatcta
     2581 caaggctata ttaaaatatt attaattttt tattttaata tagccttata ttgttatcaa
     2641 taaatgaaaa atattgtaac aatagaagaa caatagtatt atgtttatag acaaaaaact
     2701 tattaggagg taaatttatg ccaacaataa cattaaaaaa tggtgtagac gtacttatta
     2761 gagaaggtgt aagagaagat gctcaaagca ttatagattt ttataatgaa gtgggtggag
     2821 aaactcattt tctgtcattt ggaaaggatg aatataaaat atctttggag gaacaagaaa
     2881 atgctataga atcagcaaaa gcatccgata attctgttaa actgattgca tttatagatg
     2941 gtgaaatagt tgggattgca acgattgatt ctaatcaaaa agcaaaagga aagcatgttg
     3001 gagtattagg aattgttgta aaagaaaaat attggggaat tggattgggt aaaaggctta
     3061 tgttagacct tatagaatgg tgtaaatcaa atggaataac caagaagata actcttgtca
     3121 ctaatgaaga aaattataat gctataggat tatacaaaaa agttggattt gaagtagaaa
     3181 gcatactaaa gaaagaatgt tattataatg gtgtttatac tgatttaata ggaatgtcac
     3241 ttttattggg aatataatta gtgtacattt tatctcgaaa aaagtatatt aagtatggta
     3301 taattaaaaa agaatataac ttaatataat tttggaggac gaattatgaa aaatctgtgt
     3361 aaaatatcag atgattattt aaacagcaag cttaagtatt taaagttatt atcaaaacaa
     3421 tatccaagta tatcaaaagc aagtacagaa ataataaatt tagaggctat attaaatctt
     3481 ccaaagggaa cagagcattt tataacagat gtacatggtg aatatgaacc atttgtacat
     3541 gtattaaaaa atggttctgg tgtaataaag agaaaaatag aagaattgtt ttcaaataca
     3601 ataagagaca gcgaaaaaaa aatgttagca actcttgtat attatcctga acaaaagtta
     3661 gacctaataa ttaagcaaga ggaaaatata gatgattttt atagaataaa tatttataga
     3721 cttatagaac tttgtaaata tgcttctagc aaatatacta ggtctaaagt tagaaaactg
     3781 ttaccagaaa attttaaata cataatagaa gaacttttgc atgagcatgt taaaagtgaa
     3841 cataaagaag agtattataa aagtatagtt gaaacaatag ttgacatagg aatagctaag
     3901 gagtttataa ttgctatttc tacagttata caaaaattgg ttgtagatag acttcatgta
     3961 ataggagata tttatgatag aggtccaaga ccagatataa ttgtcgataa gcttattgaa
     4021 catcattgtg tagatattca atggggaaat catgatatat tgtggatggg tgcagcctca
     4081 ggtgaaaaaa cttgtatagc aaatgcactg aggatatcag ctagatatgc gaatttagac
     4141 attgtagaag acatatatgg aataaatcta ttgcctttag ccacttttgc tatagaaatg
     4201 tataaagatg acccttgtaa agaatttatt ccaaaggtta atgaccaaag tgtaacaaca
     4261 acagaaaagt ctttaatggc aaaaatgcac aaagcaataa gtataattca atttaagctt
     4321 gaaggtgagg ttatcagacg aagacctgaa tttgaaatgg aacatagact cttgcttaat
     4381 atgataaact atgatgaagg tacgattaat ttaaaaggta aaacatataa attaaaagat
     4441 acttatttac ctacaataga caaaaaagac ccatataaat taactatgga agaaagaaat
     4501 gttattgata aattagtatc ttcatttaga ggtagtgaaa aattacaaaa acatgtttca
     4561 tttttgtttt ctaaaggaag tatatattta aaggctaact caaatttact gattcatgga
     4621 tgtgtaccgt taaatgaaga tggaagcttt atgtctatga atataatggg aaaagagtat
     4681 aaaggtaagg ctcttatgga taagatggag tctctagcaa gagaaggatt tttctttaaa
     4741 gataaggcag aagaaaaact gtatggcatg gatattatgt ggtatttatg gactgggaaa
     4801 tgttcatcat tatttggtaa agatgatatg actacatttg aaagatactt tatagctgaa
     4861 aaagagactc ataaagaaaa taagaaccca tattttaaac ttagagaaaa tgagatggct
     4921 tgtaaaagat tatttgaaga atttgattta gaacttgatg aatctcatat aataaatggt
     4981 catgttcctg tggagagtaa aaatggagag agcccaataa aagctaatgg taaaattctc
     5041 gttatagatg gtggtttttc tagggcttac caaaaaacta ctggtatagc tggatacact
     5101 ttgatataca attctagaac tttgcaatta gtatcgcatg aaccatttaa ttctgctgaa
     5161 gaagccattg caaatgaaag tgatatctta tcgacaactg ttgtagtaga gcataaggca
     5221 aagagaaaaa tggtgagaga tactgatgaa ggagtaaaga tacaagagga aatagaagat
     5281 ttaaaactac ttttaatggc ttataagaaa gggctaataa aagaaatgta gatgttatag
     5341 aggcacttca tgattgaagt gtctttttgt taatacttat taaatatatt ataaagatta
     5401 ataatagtag tttatattat acttactaag tgaatccaag ataaatgggt tcactttttt
     5461 tatttataaa cattattaaa aaaatataga tgttttggtt atattccttg tcctttataa
     5521 atagaattat ataaacaaag gaggaggaca agagatgaat aaactttctg atgaaataat
     5581 aaactctctt tctacaacta taagagaaaa ggtagaaaaa gtctcaaaca ataatctgaa
     5641 catagaagaa attaggttac gctcacaaaa accattgata ctaaatgcaa actctaaaga
     5701 ttatttttac aatcaaaaaa ctatgacact tgatttaaat caacaaaact catatgtagt
     5761 aacaagagaa gatgtagagc aaacttttca aataatatgt aaatattcaa tacattcatt
     5821 tatggatgac ataaaaaaag gattcataac tttgagagga ggtcatagag tagggttagt
     5881 tggaaaagca atagtagaag atggacaagt caaaaatata aagcatatat catctttaaa
     5941 tataagggta tcaagggaaa ttattggatg ctcggataag attttaagtc atatcataaa
     6001 gggaaaaaat cagattaata atactttgat aatatcacca cctcaatgtg gaaaaacaac
     6061 tttaataaga gatatagtaa gaaatttaag taatggtaat gaagattatg gatttaaagg
     6121 tttaaaagtg gctttagtag atgagcgtaa tgagatagca ggagcttatc taggtgttcc
     6181 acaaatggat gtgggaataa ggactgatat aatagaaact tgcccaaaag acttgggtat
     6241 tacaatgctt ttaaggtcta tgtcaccaaa tgttattgtg acagatgaaa ttggaagtga
     6301 aaaagaaata aaggcattat acacagcatt aaatggaggt ataggattaa taactactgt
     6361 gcatggagat tcaatagagg atatacaaaa tagaaaagaa ttaaatagac ttctggataa
     6421 agagttattt aaaaaggtta taattctttc tgcaaaaaga ggagcaggaa caattgaaaa
     6481 aatttatgat ttagaagaaa agagatggta ttttgcaaat taaaataatt attatagctt
     6541 tcttgatagg aagtagttat ttgataggtg aacaaatata caaaacttat actcgtagac
     6601 ataaacaatt aaacgattta ataagagttt tagaaatttt gaggatggat ttatcttttg
     6661 gactttatac attagaagaa atctttaata ggataggtgg aaataaagag ttttgttttt
     6721 ggaaattttt ctatcaaata tcagaaggac ttcacaatga acaaagtaaa actttggaaa
     6781 taataatatc agaaaatatt gatgtcttaa gtaaagaaac ttatctaggc aataaagaaa
     6841 ttgaggaact gaaaaatcta atacttactt tagggaaaag cgatatagag tcacaacaaa
     6901 gaatgataga tttatctata gaaaatttga aaaaacaaac ctatgagaca aaggaagata
     6961 taaacaaaaa aggcgtattg tataaaaaat tagtgacatt cataggtata ggtatatgta
     7021 ttatattgat ttaatatggg gaggtttatt atggaaatat cattgatatt aaaggttgca
     7081 ggtgtcggta ttctaatatc agtattgaat atgatattgg aaaaaacaga tagaaaagat
     7141 tgggcaggtc ttactacatt agcaggtgta ataatcgtat tgggtatggt aataacagaa
     7201 ataagtgact tgtttaatac tgtaagaaca atgtttcaac tttactaaag aggagggagt
     7261 aatttggaaa taatgcaatt aataggaatt gcaatcattt caactacact ctgtttagta
     7321 attaaaaaag atagaccaga gatagcaaat ttcatagcta taataactgg agtaataata
     7381 ctattgtcag ttatgtttaa gctaaatttt atagtagata gtatacaaga tttggctaac
     7441 aaggctaaca tcccgactat gtatatatca ttaataataa aattaattgg gatagcatac
     7501 ttaatggaat ttgcgataca actttgtaaa gattgtgggg aaggaaacat tgcttcaaaa
     7561 ctagaatttg gaggtaaaat aatagttatg tcaatgtctt tccctatact tttgtccatt
     7621 gtagaaatgg tggtgaatat aattccatag atgtgtatct tggaatgaat ataagtatat
     7681 tagagagctt ttgttgtatt gaggtttata gagtgaagag aggcatggtt gcttatgaaa
     7741 aaaagatttt taccaatgat tataggtttt ttatttactt tcttttttgt aaatatcttt
     7801 gctattatga tatttgctaa tgaagtacca agtagtgaag ataaagaata tggtgaaaca
     7861 aaaaatagca tagataagta tatagatggt caattagaca agctagatat aaatgagatt
     7921 caagactata taaataaaga aatagttatt aatgatgtta atttaaaatc atttgtaaaa
     7981 gatttaatta gtggagaaaa aaatatctta gatttattca ataaagatgg attgaagata
     8041 ttgatgtttg atgaatttaa agcaagttta aaggtagtag cagtaatttt ggtactggct
     8101 ctgttatcat ctattttaaa gagtctggaa aactcttttt catcaggtgc agtaagtcaa
     8161 atagcaacat atataatatt tatcacaatg gtatcactta cacttattgg ttttaaagat
     8221 gtattacaga tttgctatga tgcaatagac catactgtag gtcttatgca agttataatg
     8281 ccaatattaa taacattttt acttttaatt ggattcccaa taacgtcgac tactttgaat
     8341 cctatcttca taggaggagt aacatttata aatgtatttt ttaaaaattt tctatttgta
     8401 tctattactg ttgcatttgg aattttaata ataaacaatt tatcaaaaaa tataaggcta
     8461 aagagatttt tctcctttgt aaaacaaata aattatgttt ctattggagc tatgtttact
     8521 gtttacttag gtcttgtatc catacaagga ttgtatgtaa ccagttttga taaattcagt
     8581 gtaaagactg ctaaatttgc tataggtaat tttattcctg ttgtaggtgg atttgtatct
     8641 gactctgtgg atattttatt atcttcatct cagcttataa aaaatatatt tggaggaata
     8701 gggcttatct tacttgttgg aatttgctta cttccagtaa taaaaatttt atcagtaata
     8761 gtagtgtaca aattagcagc aataattgta gagcctgttg gagaggatgg aatatctaat
     8821 tttttaaatg aagtggcaaa tttaatgata ataatgctag catcagtaat tgctataaca
     8881 gtaatgttct ttgtaactgt agctatcttg acctcgataa gtgtcgtaag tcaagggtaa
     8941 tttatatatc agtgaaaaat aattacattt aatctaatgt ttttagagag gaggatgcta
     9001 tgttagaggg cataaaggca tggatagtta gtgttttgat aggagcattt atagtaaata
     9061 tagtagatat gattttacca agttctaaga taaaacctta tgtcaaccta gttttaaact
     9121 ttatgtttgt atttattgta ataactccag tagtaggatt tttttcaaaa gatatgagtt
     9181 tagaagatag aatattaaaa tctatgggaa actacaacaa gcagtatgta gatagtacaa
     9241 atgctttggc aaaagagact ggaaataata gtctatccaa gggatatgag gatgggttaa
     9301 aagaagtact taaattaaaa cttgatgaat atggttatga tttagaagat atagagctta
     9361 atggggcaaa tataaataat ataaaaataa aagaaaagaa taatagtgca aaaagtaata
     9421 acagttccaa tgaagaaaat aaaaataata gtactaaaag agaggaagaa aacataaact
     9481 ctaatgacaa agaaaattct aaacaagtct tcaaaaaagg tacagagtat gggttgaacc
     9541 taaatgaaga aaaattaaaa aacgacttaa taaaggtgct ggatgtttct atagaagata
     9601 tacaaattga taaataggag ttgggagaat gttgaaaaac ttaaatgaca aagataagag
     9661 aaaggtatat tcactaataa ctatagcagg aatatgtgtg gtttcattag tattattatc
     9721 ttgttttcct agtagcaaaa gcgataaaca agttggaaaa acagagacta ataaaaatgc
     9781 tgaaaagcaa gtcactaaag aacaagaaaa agatgattta gagtcaaaat taacagcaat
     9841 actatctaaa attgatggtg caggagacgt tgatgtaatg gttacatttg aatcaagtga
     9901 agaaatacaa cctgctttta attcaaataa tacaacagaa acaacagagg aaaaagatgc
     9961 acaaggtgga gaaagaacag ttacgacttc tagtgaaaat aaaacaatga taacttcaaa
    10021 ttcaagtgac cctgtagtta taaaaactac agagccaaag ataaagggag tgatagtggt
    10081 agcaagtgga gcaagtgacc caagtgttaa agagacactt tatagtgcag tacaaacatc
    10141 attacaagta gctggtcatc aagtagaaat atattctaaa taaatacata taaccggggg
    10201 gataaatgaa tgaagtttaa ttataaggga agaggatttg taataataac tttaactgca
    10261 atgttagtgg tagtaggaac agtgaattat caactaagta agaaatcttt attagaaaca
    10321 tcaaaagagt ttaaggcata tgaagaagca cagttgcaaa aaaatactga tgatagttat
    10381 agtagtaaga cagaggattc tagtaatgta gataaacaag gtggtaagga aagtgctgat
    10441 attgatatag tcgatagcaa agctagtaaa gtaaaagaaa aggcaactga gacaagtaag
    10501 gaaataaaag ctcaattatc atcagagaaa aatatgaaaa aggcatctta tattttagat
    10561 atgaaaatga atagagaaaa acaaagaaat gagttagttc aagatttaaa tgaaatgata
    10621 aataatccat ctacaacaga agaatctaga aaagaagctt ctaatatgaa attaaatata
    10681 gttaaaattc aagaaaaaga acttcaaata gaaaaccttt tgagtacaaa aggatatgaa
    10741 gaagcgttag tttatataag cgataacaaa gttaatgtag ttgtaaatga agctaaatta
    10801 gaaaagaaag atgcagcaaa aatatttgat ttagttgcag aacaagccaa tgtgaagtat
    10861 gaaaatatta aacttacaaa taataatagt aataagtaaa gtataaacct cagaattaac
    10921 tgaggttatt ttcttgcaaa gtagacttgt atttggtatt attatactta taaaaatagt
    10981 tccttaaaat caggaggtaa gttttaatat ggaagataat aaatttggac aagtaaaaat
    11041 atctaatgat gtaatagcta caatagctgg tttagcagca ttagaagttg aaggcataga
    11101 aactacagca acattaacag ataagttgtt aaaaaataat ggagtaaaaa tacaaatcga
    11161 agaagaggat gttaatttag atgtaatggt tacgataaag tatggaatgt caataccaga
    11221 tacagctttt aaagttcaag aaaatgtaaa aaatactgtt gagacaatga caggactgaa
    11281 ggtttctcaa gtaaatatac acattcaagg aatcagcttt aagaaggata aagttgataa
    11341 agaagaagca aaagcaacta agaaaaatta atattagccc tctgaaaagt cagagggttt
    11401 tttgctggta attgatttta tatggataat taaacttaaa ggagaatttt gattgatgaa
    11461 aaaagatagg gcacaaaaga gtacaacaag agaatatata atgaaactta tataccaaat
    11521 aaatattaat aaagaagatt ttgaaacttt agaagataaa gtagataatt ttttaaaaga
    11581 taattcagaa catattataa atagatataa agaacttgca cttcaatact caaaaaatac
    11641 aaatttaaag ttagaagata ctgaaattga agatgttata gacaaaaaat atataaatac
    11701 agtatgcaaa gcattgaaag aaaatcatga caaaatagat gagttaataa ataagcatgc
    11761 taaaaactgg acagtagata gaatgccaaa ggtagatgtt tctatactta gattgtctgt
    11821 gtgtgaaata ctttatttag acactcctaa taaagtatca ataaatgaag cagtagaact
    11881 tgcaaaaata tattgtgatg ataagtctcc taaatttata aatggtatat tgggaagtgt
    11941 tgttgatgaa attgggaaat aatataataa ttggaattga tactagctgc tatactactt
    12001 ctatagcagc tatctcttta gataagaaag ttatattcaa tgaaaaaata atgttagaag
    12061 taagggataa ttcaaaaggt ttaaggcaaa gtgaagctgt atttcaacat atcaataatt
    12121 tagggattct tagtgatagg ataaaatctt ttaaagataa gtttaatgta gaaggagttt
    12181 gctcatcaaa aaaaccgaga cctgttgaaa actcatatat gcctgtattc aatgtaggtc
    12241 ataattttgg aaaattattg tctagtatat atggttgtag attttatgag actactcatc
    12301 aagagaatca tattgaagca agtttattaa atagtaaatt aaaaaataat aataaattta
    12361 tttctgttca tatgtctggt ggtactacag agatactttt aacaagcaaa caagatagtc
    12421 atcacaatgt atgtgatact aatttaggta aaattgccaa aataagtatt aaaaaagatg
    12481 ataaaagtaa gttatacaat aattttggat ataatataga tataattgga ggaagtaaag
    12541 atattagctt tggtcaactt atagatagag taggaattaa gcttggatat aaatttcctt
    12601 caggaaagta tttggatgaa aatgctttaa attgtaatct taaaatagaa agtggtctga
    12661 aaacttctgt aagagatggg tatatgaatt tatcaggatt agaaaatcag gtaaataaga
    12721 ttataaatga taatggagat aatactaatc aaaaagaata tatatctaaa ttagtattag
    12781 attcagtagt tagaaatatg tttaaatctc tagtgtattt atgtgaaact tataatgtaa
    12841 atgaagtagt atttgctggt ggagtttctg caagtaagta tattcttaga gaattaagca
    12901 tgaagttaag aaaaaagcat atagaagctt attttacaga accacaatat tcaactgata
    12961 atgctgtcgg gtgtgctata ataggattga ataacttttt aggagaaaga gtatgaaact
    13021 aagagcttta gatataagtg aagcaaactc atatattaaa agaattttaa taaacgaccc
    13081 aatactttct aatcttaaag ttaaaggaga aatttctaat tttaaggttc atagtagtgg
    13141 gaatgtatat ttatcactta aagatgaaac atcaaagtta aactgtgtaa tttttaaaag
    13201 taattttaat cgcaatttaa aattagataa tggtgtaaaa attatagcta atggatatat
    13261 atcagtctat gaaagagatg gtgcgtatca attatacatt aatgaaattg aaatagaggg
    13321 aataggaaat ttgcacatag agtttaatag attaaaggaa aaacttaata aagaaggatt
    13381 atttgaccct aaatacaaga tacctatacc taaaatgcca aactcaattg gcgttataac
    13441 atctccaaca ggtgctgtta taagagatat aataaatgta ataaaaagaa gatatccgaa
    13501 agttaatata aagctttatc cagtaacggt tcaaggagat aaatcagctg aagaaatctg
    13561 tgaagcaatt agatttttca atcatatgaa gaatgttgat acacttatag ttggtagagg
    13621 tgggggttca atagaagaac tttggtcttt taatgatgaa atggtagcaa gagaggtttt
    13681 taattctcaa attccgataa tatcagcagt tgggcatgaa acggatttta ctatttgtga
    13741 ctttgtgtct gatatgagag ccccaacgcc atcagcagca gctgagattg ctactccatc
    13801 attggatgat ataaactata aacttggaaa cataaaaagt aggatgagta agtctcttac
    13861 aaatcaaata gagctagacc aatataggtt agaaacagta tttaataaga taaataacta
    13921 tttagattcg tacacaataa aagataaagt tatacaatta gacaaaatat atgataaaat
    13981 aatttttgga atagaaaata atttaaaatt agaagatgaa aaattagtaa aaattggagc
    14041 attactccat aatttaagtc cattagctac tatggataga ggttatagta taactcaaaa
    14101 aaatggaaaa gtaataaata gtataaaagg attaaaaata aaggatagta tagatatagt
    14161 attaaaagat ggaaatttag aatgtatgat agataaaatt gaaaataaag agggatagta
    14221 tgaatttaac ctacgaagaa gcttataaaa gattagaaag tattttaagt gaattagaat
    14281 ctaaaaatgc tagcttggat gaatctttaa gcctatatga agaaggtata agcctttata
    14341 aacattgtaa taagctttta gatgatgcta aattgaaaat aagtaaattt aatcaattgg
    14401 gaatagaaga agactttaaa atagaggagg aataaaattg gaatttaaac aatgtctaaa
    14461 agaaaaagca agttttgtag aaaaagtact taaagaatat atgcctaaag aagaagggta
    14521 tcaaaaaact gttattgaag ctatgaacta tagcttaagt gctggaggta aaagattaag
    14581 accaatactt acattggagg cttgtaaaat agttggaggt aatgaagatg aagctattcc
    14641 ttttgctatt gcaattgaaa tgatacatac atattcacta atacatgatg atttgccagc
    14701 tcttgataat gatgatttaa gaagaggaag accaacaaat cataaagtat atggagaagc
    14761 tatggggatt ttagcaggag atgctttgtt aaactatgct tttgaagtaa tgcttgcagg
    14821 ctcaatcaat aaagaaaatc cagaaaagta tctaaaagct ataaatgaaa ttgccaaagg
    14881 tgctggtata tatggaatga taggtggtca agtagtagat gtagaaagcg aaaataaaca
    14941 aattgaaaaa gaaaaattag attatataca tatgaataaa actgctgcaa tgatggtagg
    15001 ctgtatgaga gcaggagcta ctataggtgg agcaaattct gaacaaatgg aagaaataac
    15061 taaatatgca aaaaatatcg gattatcatt ccagatagta gatgatatac tagacatagt
    15121 aggagatgaa gctaagttag gtaaaaaggt tggaagtgat atagaaaatc ataagtcaac
    15181 atacccatct cttttaggtt tagataaatc taaagaaatt gcacataatt tgattgatga
    15241 agcaaagaaa agtatagaaa aattatctga tgatgtagat tttttaaagg gattggctga
    15301 atatataata gatagagaat actaaaatat acagctaaaa tcgaggaggg aaaaatggat
    15361 tttttttcgg agatttttaa caatggggct ttgggaataa gtctgattgc ttgtttctta
    15421 gcacaattta taaaaatatt tactggtaag gaaaaaagaa ttgagttatc aagaatactt
    15481 atttcaggag gtatgcctag ttcccatagt tcttttgtta caagtttagc tacagtggta
    15541 ggtattgaaa aagggtttaa ttctactgat tttgctataa ttaccgtgct tgccttaatt
    15601 ataatgtatg atgcagcagg tgttagacgg gctgttggta aacaagctac tatattaaat
    15661 caaatggtgg cagatataca gcatggaaag catatagagc agaagaaatt aaaagaatta
    15721 ataggtcata caccactaga agtttggttt ggagctttac ttggaatagt aacagcttta
    15781 atattgatgt aaattgaaaa tttacagaat aaaggtgagg ataatatgta taaatattta
    15841 gataaagtaa attctccaaa ggatataaaa aatatgagta tagaagaaat ggatttactt
    15901 gcaaaggata taagaaagtt cttagtaaaa tctgtttcaa agacaggagg acacttagct
    15961 tctaatctag gagtggttga gttaacatta gcacttcata aagtatttga tagccctaaa
    16021 gataagattg tatgggatgt agggcatcaa tcttatgttc ataaaatagt tactggaaga
    16081 aaagattgtt ttgtatcttt gagacagttt aatggactaa gtggttttcc aaaggagaat
    16141 gaaagtcctc atgatatttt tgatacagga catagcagta catctatttc tatagctaca
    16201 ggtattgcct gtgctaggga tataaaaaaa gaaaactata gtgttatatc tgtgatagga
    16261 gatggttcta taacaggagg tatggcactt gaagctttaa atcaattagg atatatagat
    16321 accaatatga tagtaatcct taatgataat gagatgtcca tagataaaaa tgttggagga
    16381 atgtctaaat atctgtcaag tattataaga aactcaacag ttgagaaaat gacagatgaa
    16441 gttgataaaa ttttaaatgt tacacaaaca ggtgaaattt tatctaagac tgcacataga
    16501 tttaaagata aactaatgta tagcttttct cctcaggact gttcattttt tgattcatta
    16561 ggtataagat attatggacc tatagatggc cataatacta aagaactaat agatatattg
    16621 agaaaagcta aacacaaaaa agggcctgtt cttttacatg taattacgaa aaaaggaaaa
    16681 gggtacagat ttgcagaaga acaacctgat aaatatcatg gagtatcaaa gtttgatata
    16741 aagacaggtg ttacatctgc aaaagttaag tctatgtcaa ttagtgttgg agaaaaactt
    16801 gttgatatgg ctaatagtaa tgaaaatata gtagcaataa cagctgcaat gccatctggt
    16861 acagggttaa acctgtttga aagtgcatat ccaaaaagat attatgatgt tggtatagct
    16921 gaacaacatg caacaggatt tgcagcaggt cttgcaaaaa atggtatgaa accttacttt
    16981 gctgtgtact catcattttt gcaaagggca tatgaccaag ttattcatga tgtatgcata
    17041 acaaaaaagc cagttacttt tcttatagat agagctggat tagttggaaa tgatggagaa
    17101 actcatcatg gtatgtttga tttaagttac ttaaattcta ttccaaatat tgtagtaatg
    17161 gctccaaaag acacaagaga gatggagctt atgatggatt tgtcattaaa attagattgt
    17221 ccactagcaa ttagataccc aagaggaagt agttattacc tagataaggg agagtatgga
    17281 gagattgtac tgggtaaata tgaggtatta gatgacggtc aagatacagt catactttgt
    17341 attggaagta tggtaaaaca tgctttagag gctaaagaaa tattatcaag agaaggaata
    17401 aatccgacaa tagttaatgc tagattctta aagccaattg atgaaggtat gttaaaagca
    17461 ttattaaaaa atcataaaaa tgtagttact atagaggata atattgtaac tggaggattt
    17521 ggaagtagaa taaataagtt tattatagat aatgaatata acgtaaatat acttaatata
    17581 gcaataccag aagaatttgt aaaacatggc aatatagatg aattatatga ctttgtagga
    17641 ttatcgccaa aaagtattgc tgataagata aggaaattag ttattgagta aaaatctaat
    17701 gtagatattg taaattaaaa tttatataaa atatagaatt tatcaattag aataaatatc
    17761 aagtgtgtta cttcaagcta ctttagttat cattaaagta gcttttaaca gaaaggtaat
    17821 tgtatgaaaa aaagaataga tttattatta gtagaacaag ggtactttga aagtagagaa
    17881 agagccaaaa aagcaataat ggctggattg gtttttgttg ataatcaaag gtgtgataag
    17941 gctggtacag aagtaaaaga agattgtagt atagaagtaa aaggaaatcc aataccatat
    18001 gtcagtagag gtggtttaaa gcttgaaaaa gcaatgaaaa actttgattt gactattgat
    18061 ggaaaggtat gtatggatat aggggcatcc acaggtggtt ttacagattg tatgctcaaa
    18121 aatggagcaa taaaagtatt ttctatagat gttggttatg gtcaacttgc atggaaattg
    18181 agacaagatg atagggttgt gtgtatggaa agaaccaata taagaaatgt gacaatagaa
    18241 gatacaaagc agtttgctga ttttgcgtca attgatgtgt cttttatatc gttaaagtta
    18301 gtacttccaa aggcaaagga gttagtacgt catgatgggg aagttgtcgc tttaattaaa
    18361 cctcaatttg aagcaggaag agaaaaagta ggtaaaaaag gagtagttag agaaaaatct
    18421 acacatatag aagttataaa aatgatttct gatttctcag tcgaaaatgg atttgagatt
    18481 ttagggttag atttttctcc tattaaaggt cctgaaggta atatagaata tctaattcat
    18541 ttgagaaatg gaaatgaagg atatgaattt gatggtgaga catacaataa caaaatagta
    18601 gaagttgttg aagcttctca taatttagac aaataggtta aaagggggct tagatttgat
    18661 cctcgaatta tatatgaaaa attgtgcctt agttgaagaa ctaaggttga atattgataa
    18721 aaacttaaat atacttacag gtgaaactgg ttcaggaaaa tctataataa tagatgcact
    18781 tggactttgt ttaggtgata aatatgatag gtctttttta agaaaaggaa cagataaggg
    18841 acttgttgag gctgtatttt tttcagacaa tagatatctt aaaaaaatat tagaagaaaa
    18901 tgatataagt atggaagatg ataatttatt ggtaataact aggttgatat actcagatgg
    18961 taagagtact gctagagtaa atggaaggac tgttaaagtg tctcttctta aagaaattgc
    19021 atctacactt atagatatac atggtcaaca tcaaaatcaa gctttgttta ataaagatac
    19081 tcatttaaaa tttctagacc tttttggaga aaatgaattg gaagagttta aaattgctta
    19141 taaaaatgtg taccataagt atagtgaagt aaaaaaagct ttaaattgct tgacagaaaa
    19201 taaagatgag atgcaaattc aacgagaaat agatttatta agatttcaaa taaatgaaat
    19261 tgaagctgca aatctaaata agaatgaata tgaagattta ttaaaacaaa gagaagttta
    19321 tagaaatagt gaaaagatat ataataatct aaattcgagt tatagcaagt tacataatgg
    19381 tgagtataat gttattgacc taataggtct tgcatctaag gaacttaatg acatatctaa
    19441 atatgacagt gttttaagtg aatatagtga tactgtagaa agaatcatgt atgagttaca
    19501 agacatatct ggtgagataa gaaattacaa agataatata gactttgaac cttatgagct
    19561 agagcagatt gagcttagaa tcgacgagat taataactta agaagaaaat atggagacag
    19621 tatagatgat atatttgagt attatggaaa aattaaagat agattagatg aaatattaaa
    19681 cagagatgaa agagttgaac agttaagaag caaactgatg aatatagaag aagatttaaa
    19741 aataaaagcc agtaaattga ctaaagcaag aatagaagta gcaactactt tagaaaaaat
    19801 acttcttgat gaattaaaaa gcttaaatat gaaaaatgta atgttcaagg ttaattttga
    19861 aaaaggtcca tttactttag atggagtaga tgatattgaa tttatgatat cttttaactt
    19921 aggagaagat attaagccta tatataaagt tgcatcaggt ggtgagatgt ccagatttat
    19981 gttagcgttt aaaaccatat tggctgatat agatgatatt gatacattag tgtttgacga
    20041 gatagatact ggaataagtg ggatagcagc acagatagtt ggtgagaaat tgagtgatat
    20101 agcaaagaaa aaacagataa tatgtataac tcatttacct caaattgctg caaatgcaga
    20161 cactcattat tgtatagaga aagatacttc aaataatagg acgtttacca atgttagtaa
    20221 gttaaatgaa tctcaacgaa aaaatgaaat tgccagactt atagcaggta acaatataac
    20281 agaaaaaact attgaacatg caagtgaaat tattgagttg gctaaaaaat gttagtgaaa
    20341 aacaagcatt ttaataggtg taaagaattt ttatagaaat gctttacatc tattttttat
    20401 gatgaaatta ttttaaaata tctagtgcct atattgaaga aaagtaggtt tgaaaatgga
    20461 atgaactttg tataaatttg taactttgaa atttctaaat gcttaagtat aatattattt
    20521 attaaggtag tagtgagggg aggcataaaa tgaataaaaa agatactatg cacttaattt
    20581 ttataattgt aatttttatt ttattatggt atattgtggt agcagtaagt gagattaata
    20641 aaaatattga aagttgtagt aacaaattag attatttgtc acaagagctt atgagtacta
    20701 agtcggatat tagtaatact ataaatgaag aaatgtcaaa aagctatata acaaaaagta
    20761 ttgacttaaa agtgaaaaaa atcgagaaaa aagaatgtat tattgatata gatgttcaat
    20821 taagtaaact tggaaaaaat ggaaaagtat tttttatgta taaagaagat agtgataaat
    20881 ggaatgaaac tgagatgaca aaacatggag aattatcata tgcatgtgaa attaagataa
    20941 atacaggaag tgaatatgac tataaagtag ttacaagtgg agctatatct gaaagttctg
    21001 atgtgcaaaa attgactaaa tctgattata tgcctgaaca acccatattt aattctggga
    21061 taagagataa tagagaatac tttattgaaa tagtagaaaa tagtaatctt ttcaatcatg
    21121 agagtgaaaa gtcaaaaaat aaagtttatg ataatattag attagaaaaa gtagatatta
    21181 ttgtaaatga aggaaaaaaa gacactatat acaaagccaa attagctgaa ggcttagatg
    21241 atggcaaaac taataattca aatagaaatc aagtgaatta tgaagtaagt tttcctaaaa
    21301 gggatattga tgacataatt tatattaaag ctaaacttac atacaataat ggagtagaat
    21361 atacaaagga tataactgaa gatataacta tgggattaca agatttagaa aaataaatac
    21421 atatttaaat aaatattcaa atagatttgt tcaataaaat agagtataaa taaaactgta
    21481 gtagttttac ttatactcta ttaaaaaatt ataattttaa ttccatatat ctagcccctt
    21541 caattggatt ataagtataa ggttctatat cataaaaacc aaacttttcg tataatcctt
    21601 gtgcactttt catgcttgga agagtatcta atctcatgtg tgtatatcca aattttttag
    21661 cttcttcaat gatttcttcc aatagtattt ttcctatttt taatcctcta aattgatttc
    21721 ttacatataa tctttttaat tcacatattt taccttcaag tttttttaaa gcaacacaac
    21781 ctgccaaatt ttcatcaaca aatgccaata tcaatgagcc tgaaggtttt ttatattttc
    21841 ctggaagtgt ttttaattca ttattaaagt cttgaaaaca taagtctata tttagagaat
    21901 ttgaatattc agtaaataga agttttacgt tttcaaggtt ttctaaatct ttttcttcaa
    21961 caaattgtaa agttatattt tgagccattt tatccgccat tatatacaca tgtttatata
    22021 atgacttcac ctccttgata tatgtatatt tttcttaata tcaattagat tttaatacca
    22081 ttactttttc accaccatag tcaagctctc cattaaactc aaatccaaaa tctttataaa
    22141 gcttaatagc cattttattg tcttcgaata tactaagata tagttcatca cagttatatt
    22201 catttttaag atgatttaca agaaaatcta atgatgcttt tccatatcct ttaccttgat
    22261 attttgaatc aatcataaat ctatcaagcc aaactctatc atcttctaaa cgaccataca
    22321 tagcaaatcc tattaaagaa ttttcatcat atattccaac aggtacccaa gctgttataa
    22381 atttagattc agcaatagaa agtgcattgc tttctatata atctaactga cttttatcta
    22441 cagacaaact aactaaatca tgccaattat ttatgtctgc aattcttaca tttaatttca
    22501 agatattacc gcccttcaaa ctttttgtta caatctaata ctatcatatt atgtattaaa
    22561 aatcacttat taagatttta aataaacttt ttaaatgaat aaattctcag gaaaaaggaa
    22621 ttataaatac ataataatac agcataggag tagatggata tatgaacaaa ataaataaaa
    22681 atttcaaaaa tatttggata atattaggag ctgtatcgat ttttaccatg gttataattt
    22741 ttaataaaaa ttgtaatgtt aataaagaga ctcaaataga agcttttaca attaagaatc
    22801 atgataaaaa atttgtatat cctatgggaa atataattgg agtaaaagca aatacagatg
    22861 gtgtattggt cttaggatat gaggaagagg atgtagacta tattggaggc atacaaatcg
    22921 gtgataatat agtaaaaata gataataaaa ggataaaaaa tagtcaagat gtatcagaga
    22981 ttttgaataa gattaaaaaa agtaaagttg aagtgacttt tgagagaaaa aataagtata
    23041 aaacagaagt aatagagact aaaaaagaaa atggcaagta taaacttgga ttatgggtaa
    23101 gagataaaat atctggaatt ggaactatga ctttttacga ccctagtatg gaaaagttca
    23161 aagcaattgg tcatgccata aaagattcgg atactaatga actattaaag ataaagcaag
    23221 gatatatata taagccagaa caattaaaaa tagtaaaagc tagtaatgaa aaagtaggta
    23281 aaataaaagg agattttaat gacagcaatt taatgggtaa tttctcaaat aattcagagt
    23341 taggtataag tggtaatatt acagaaaacc ataataaaga gtttaatgta gcaaataaag
    23401 aaaagcaatt aatagaagtt ggaagaccgc aagatgtaaa aataggaggt gcagtaattc
    23461 tttttgagga taaaaacaaa aatataacaa gctatgatat aaaaatagaa agtatagtat
    23521 atgataaagg aaattataga gatatggtaa taaaagtagt agatgacaag ttattggaat
    23581 acacaggagg tatcgtacag gggatgagtg gagctccaat aatacaaaat aataaaatta
    23641 ttggtgcaat aactcatgtt tttagagata atccgaaaaa aggatatggt atttttatag
    23701 atgaaatgat aaaattgtag gtgaggcatt aaaaaatttt attattttat caattatcta
    23761 ggaggaatat aattttggag tgtcgaatat gctttagagt agataattag gaagcaattg
    23821 tgtaaaaagt ttagttttct gtaataagaa gatgtttttt aatgggggga tttttagtgg
    23881 aaaaaatcaa aatagtttta gcagatgaca ataaggattt ttgtcaggta ttaaaagagt
    23941 atttgtctaa tgaagatgat atcgatatat taggcatagc taaggatgga attgaagcat
    24001 tagacttagt aaaaaagaca caaccggact tattaatact agatgtaata atgccacatc
    24061 tagatggatt aggtgtaatt gaaaaattga atactatgga tatacctaaa atgccaaaaa
    24121 taatagtact atcagcagta ggtcaagata agataactca aagcgcaata aatctaggag
    24181 cagattacta catagtaaag ccatttgatt ttgttgtgtt tataaacaga attagagagc
    24241 tagtttctaa tagagttaca caagttgagc caaaacctag accagttcaa gagactcaaa
    24301 tgactagaag tgattttgtt aagaatgtag gaaatataga gacagaaatc acaaatataa
    24361 tacatgaaat aggagtacca gctcatataa aaggatattt atatttaaga gaagcaataa
    24421 aaatggttat tgataatgtt gagcttttag gtgcagtaac aaaagagtta tatccaagta
    24481 tagctaaaaa attcaataca acaccaagta gagttgaaag agcaataaga catgcaatag
    24541 aggttgcatg gagtagagga aaagttgaca caataaatca attatttgga tatacggtac
    24601 acaatactaa aggaaaacca actaattcag aatttatagc aatgattgct gataaattaa
    24661 gactagaaca tagtatggtt aaataaacaa gacataaaaa gtaaggcttt ttaattaagg
    24721 cattggctat aaatgcgtat tacaagcagc gaaacggtat aaccactagg gttataccgt
    24781 ttcgctattt taataaatat aaaaatttta tttattattt gcttactata tcaatataat
    24841 aattttatta tactatggat atagtatgtg tctttacaag ttgtaaactg acagtggttt
    24901 attttttaat ataaatattg actttgatgc aggtaaactt tgtattttta agcgtattat
    24961 ggaatatgtt aaataaaaaa atgatgaaat atagtattgt aaatgccaaa gatgcaaaac
    25021 aaacttaaaa catttatttt attgttaagt aatgctataa tataatgtga ttttaataat
    25081 gatagtggag gtttaaatat gagagtcgag gcccctataa aagtagatcg aaaaaccaaa
    25141 aaacttgcta aaagagttga aagtggggaa atagcagtta taaatcatat agacatagat
    25201 gaagttgctg caaactcttt agtagaagct aaaataaaac ttgtcataaa tgcggctcct
    25261 tctataagtg gtaggtatcc caataaaggt ccagggatat taactgaaaa taatatacta
    25321 ataattgata atgttggaga agagttattt gaagaattaa aagaaggaga aactatagaa
    25381 gttgtagatg gaaagatata tagaaaagga aaatttttag gtgcaggaga agttttagac
    25441 aagtatgagg tttcacacca aataaaggct gcctatgaga atttagctgt agaattggat
    25501 agatttatag ataatactat agattatgct aaaaaagaaa aaggatttat acttggagaa
    25561 gtagaaattc caaaagtgaa aactaattat gctaataaac atgtattgat agttgtaaga
    25621 ggtcaggact acaaagaaga tttaagtact atgctttctt atatagaaga ggtaaagcca
    25681 gtattggttg gtgttgatgg aggagcagac gctttaattg agtttggata tactccagat
    25741 gtgattgtag gtgatatgga tagtgtaagt gatgaagctt taaaaaaggc tagtgaaata
    25801 gtagtacatg cttatacaga tggaagagca ccaggtctta aaagggttga ggaattaggc
    25861 ttagatgcag ttgtatttcc tgcaccagga actagtgaag atatagctat gcttatcgct
    25921 tatgagtata aggctgagtt aatagtagca gttggtacac attcaaatat gattgatttt
    25981 cttgaaaaag gaagaaaagg tatggcaagt acttttttag ttagactaaa aataggttca
    26041 aagcttatag atgctaaagg cgttaatttg ttatacagaa gtaaattaaa aataaagtac
    26101 atatgggcat taatagcaac agcattattt cctgtgttgg tagttgcatc attgtcacca
    26161 ggagttcaac agttcataca actaatgcaa ttaaagttta gagtattatt acagatgtaa
    26221 attttaggag gataaatatg catattaata tgaaatatta tatagtcact ataggggcta
    26281 tatttatagc tttgggtata ggcatgttag taggatttaa tttaaataat aatcaacaat
    26341 taagtgaaca acaagcaaac ataataaatg atttggatga taagtttgat atcttaaaag
    26401 aaaaaaatga taaacttgac agtgacttag caagtgtaaa tagagattat gaagaggcag
    26461 ttaattttat aaataaaaat gtagataaga ttttagctgg ttcgctgaat ggtaaaagta
    26521 taggaataat atctgctaat gaaaatgacg attatacaaa aaatatagaa gacataataa
    26581 ataaatctaa tggaagcatt gcatttaata taatattaaa agaaaacata acaaatccag
    26641 aaaagttaaa ggaaatgtca actaaactag gtacagatgt aaaaaatgca aatgatgcag
    26701 ttaattatat aattgacact cttaaaaagg aagatgcaag tgacatttta acatatcttc
    26761 aagaactaga tgtaataaag tttaatttta ttggagatac atatctaaaa tatgattccg
    26821 ttgtaatagc tggtggaaat gatgcaaaag acagtacaaa acagtttgaa aaaatagaaa
    26881 aatttgtagt ttcaaaactt aaatcagaga ataaatattt agtagaagtt caaaatacag
    26941 gagttaaaac ttcttatgta gaactatatt ctaaaaacaa agttgcaact atagacaata
    27001 ttgatgaagg tataggaagt atatctctag ctattcttct acaacaaggt aatatagttg
    27061 gtaattttgg tagattggat actgcaactt cattgttgcc atctattaaa tagaaataat
    27121 tttaaggaga gaaaaaatgg attcttatat aagtataata ataccagctt ataatgaaga
    27181 aagtagaata aaatatacat tagaaagcat attggacatt aagcaaataa atgaaataat
    27241 agttgtggat gatggttcaa ttgacaatac tgctaaggtt gtatctgagc tagaaaatga
    27301 aaagattaaa ttttttaagt tagataaaaa tagaggtaaa ggatatgctt taaattatgg
    27361 tatcaaatta gcaatgaaaa atgcagatat tataggtttt ctggatggag atttaggaag
    27421 ttctgctaaa gaagttgaaa aattaatagt acctatatta aatggtgagg cggatgttac
    27481 aatagccaag tttcctcctg caaagagaaa aggaggtttg ggatttgtaa aaggtttagc
    27541 taaaaattct gtgtttgaga tgactggtgt tgagttagat gcaactttat caggtcaaag
    27601 aatatttaaa agagaagttt tagaaaagtt tgatgaaata ccttttggat atggagtaga
    27661 agttggaatg accattgata tattaaagta tggatttaaa attaaagaag tattagtaaa
    27721 tatgactcat aatgaaacag gaagaaattt aaaaggtttt attcatagag ggaaacagta
    27781 ttatcacata aaaaaggttt taggacaaaa aaagaaagaa tggaggtaat atcttagtga
    27841 aagattatat actttatacc attttattat taactggatt atttggaacc tatgctgtaa
    27901 ttccactatt taaaaattta ttgataaatg gaaatgtact tagaccaaac tataaaaaag
    27961 atatgatacc agtaagtatg ggaattgtat ttttgcctat gattgtaatc aatggaataa
    28021 tacttgcttt ctttactaca gaatttaagg atttagcgta tatatttatg tttatgtttg
    28081 gtatgatatc catgtttttt gcagggatac tagatgatgt cattggaaat agagatgtaa
    28141 gtggtttaaa ggggcatttt aaaagtttat ttaaaggaag tcttacaaca ggaggtttta
    28201 aagctttatt tggtggattt gtagggctta tagtttctgt tgctatatca aaagacataa
    28261 ttgatataat agtaaataca ctaataatag ctctatcaac taatttaatg aatcttttgg
    28321 atttaagacc aggaagggca ataaaagcat atttattcat aatggtaatt atatttttaa
    28381 cattgacagg atttgttcaa gttcttccat tacttatagt accaaatgta cttgcatact
    28441 ttaattatga tttaaaagca agggctatga tgggagatac tggttcaaac gtccttggaa
    28501 tatctattgg aatgctcatt tcttttgggt atccatttaa cattagatta ggatggttaa
    28561 tatttgtagt tattatgcat atattcactg agaaatactc attaactaag ataatagaaa
    28621 aaaataaact attaaatttt atagataaat tgggaaggta gtattatgat aagcaaaaga
    28681 gtggggattg ttgaatcaat agtaagtcaa actgaaacac tagatgatat aagagtaaat
    28741 ataaatggag aaatacaaag ggcatataat tatcccaaaa tatctggaac tataaatata
    28801 ggtgacgaag tggttttaaa tacaacagca gtagaactga gcttaggtac aggtggatac
    28861 cattttgtaa taactaattt aaataatata gaatctactt taactgaagg tggacatata
    28921 atgaaactta gatatacgcc tcttcaaata aaagtggatt ctgttgagga acaagaaagt
    28981 atttatcatg ataaatttgc gaattttaaa ggattagaag gtctttctgt tgtagtaggg
    29041 actcttcata gtatgttaac tccatttgca gcttcattta aaagaaataa tccaaataaa
    29101 aaattagtgt atataatgac tgatggagca tctcttccaa tttatttaag taaaaatgtg
    29161 gatactttaa aagaaaaaaa attaatagat agtacaataa ctataggaaa tgcgtttggt
    29221 ggagattatg aatgtataaa tatatatact gcattaatta cagcaaaaga aatactaaaa
    29281 gcggatgcag tatttgtcag tatgggacct ggaattgctg ggacaggaac aaaatatgga
    29341 tttacaggga ttgaacaagg ctctattctt gatgctgtaa aaaaacttga aggaagagca
    29401 attgcaattc caagaattag ttttgctgac aaacgagaaa gacatcaagg tatttcacat
    29461 catagtatga ctgtatttaa agaaattgta aatgtaaatg tagacatacc aataactata
    29521 tatgatgatg aaaaattgaa ctttataaaa gagcaaatca atttaaatgg tttagacaaa
    29581 aagcataata taatttatat agataataat aaaacgaaag aggatttaga ctattttaat
    29641 ctaaaagtaa agagtatggg aagaaattat gaacaagatg aggcattttt caaagctgca
    29701 agcacagcag catattactt aatggaggtt taagatggta ttagaagaaa aaactattag
    29761 tagtgatagg gtttatacag gtaaagtaat tactttaaag gttgatactg ttgaaattcc
    29821 agggcaagga tatcaaaaaa gggagttagt agaagttggt ggagcagtag gaatagttgc
    29881 aatcacagat gataataaag ttgtgttagt taagcaattt agaaaaccta tagaaaagcc
    29941 aatctttgaa attccagctg gtaaactaga aaaaaatgaa agtccaaaag aatgtgctga
    30001 aagagaatta aaagaagaaa cagggtatag tgcaaaaaat attaagctaa tacataaatt
    30061 tttcacttca gcaggttttt ctaatgagat aatgtttgta tatttagcaa caggtcttac
    30121 tccaggagag aataatttag atgcagatga gtttttagat gtctatgaaa ttgagcttga
    30181 agaagcatat aacatggtat taaaaaatga cgttgaagat gcaaaaacat ctattggatt
    30241 attattagta aaagatatgt ttaaaaatta atatttataa aatgaataac cctttgtcct
    30301 aaagcatatt attttataaa ggacaagggg gaattaaatt gagaagaaca tatagaaaaa
    30361 atgattttag agaattaaat agacaaataa ttattatagg attgttgttt atgatgtcta
    30421 ttgttgttgg gtcttacata aataaaatat tgccaggttc tagtaacaat atattaaaca
    30481 atataaatcc tgctgtagag tattataatc taaatataag cataaaagat acagtgatac
    30541 aaaatttaaa atcagatgca atctttatgg gctcaatagc tctattaagt ctatttgtag
    30601 taacaattcc agcagtgttg gtagcttttg tattaaaagg gatgtcaatt ggttatacaa
    30661 taaatagttg tatattggca ttaaaattta aaagtataaa aatgatttta ataatattat
    30721 ttaaaaattt gattattatt ccatgtgcaa tcatattagc attaattagt ttaagttatt
    30781 ttaaagaaat ggtttatgaa tttaaaaaga aaaatagaaa aaatatgcaa tttttaatta
    30841 agaggtatat actaaatatt ataattataa tagcactatc attggggttg caattaatat
    30901 taaatactgc aagtattggt attattaagt tcctagctaa ataaaggagt ttataatgga
    30961 tattatagag ggatatatag attacataaa agataaaaaa aaattatcag agaatacagt
    31021 ggcatcatac tttatggata taaaaaaata tatggaatat ataaatcaaa aaagcattaa
    31081 actagtagat atagtagaaa atgatattat aagttactta ataaagttgg aaaaagataa
    31141 tgtatcaata gctacaatag cgaggatgat atcctctata aaatcttttc atgattattt
    31201 atttttaaat catatatgta caaataatcc tgcaaaggat ataaaaaaac ctaaaataaa
    31261 aaaagaaaat ataaatatac ttacagaaga agaaattgaa agactattaa attttccaaa
    31321 gttaacaaca cctaaattga ttagggacaa ggctattttt gaagtattat atggtactgg
    31381 tataaaagta tctgaattag tagagatgaa tatagaggat attgacctag atattgatta
    31441 tatatattgt aattcatgtt gtaaaaatca aagagtgata ccactttgtg atattacaaa
    31501 actttactta gaaaagtatc taaaagaagc aagacctaaa atggctgtag aaggtgaaaa
    31561 atcattattc gtaagttcat taggtcaaag gtttacacga caaggtcttt ggaaagtcat
    31621 aaaaaaatat tctaatctag ccaatataga taaaaatata aatcctacaa tgcttagaca
    31681 ttcatttgct attcatttgt taaatgaagg tgctaacatt gctgttgtaa gtaaaatttt
    31741 aggaaatgtt aatttatcta gtttgcaagt atatttaaat catatagata aaaacgtgag
    31801 aagagaaata aaggagaaac atcctagaaa tgatgttgat gtagaattac aaaaagcaga
    31861 agctaaatag actatatagc aaattttata atttagaagg agcatattta tatgagcaga
    31921 gtaatttgga ttgtaattga tagtgttggg ataggagcat tgcctgatgc tgaaaaattt
    31981 ggtgatagta aggatgtaag tacccttgga aatatattta aggaatatcc agatattcag
    32041 attcctaata tgagaaattt aggtatagga aacatagatg gtatagattt ttttgaaagt
    32101 ataaaagaac caataggttg ttttggaaag tgtaaggaaa tgtctcaggg aaaagatact
    32161 actacaggtc attgggaaat gactggtata atagttgaca aaccatttaa gacatttgaa
    32221 catggattct ctaaggagat aatagaagag tttgagaaaa aaactggaag aaaagtagta
    32281 ggaaataaac cagcttcagg aactgtaata atagatgagt atggagaaca tcaaataaaa
    32341 actggagatg taatagtata tacatcagct gatagtgtat ttcagattgc tgctaatgaa
    32401 gaagtaattc ccctagaaga actctataat atgtgcaaaa tcgctaggga aataatgatg
    32461 ggggataatg cagtagctag agtaattgct agaccattca taggtaagaa aaaaggtgag
    32521 tttgtgagga cttcaaatag aagagattat tcactagacc catttgaacc aacagttctt
    32581 gataatataa aagaatcagg acttgatgtt ttagcagttg gaaaaataga agatattttt
    32641 aatggaaaag gaattacaga tgctatccac actaaaagca atatggatgg agtggatgaa
    32701 actttaaatt atatgaaaca agataataaa ggtctaatat attcaaatct cgttgatttt
    32761 gattctaaat atggtcatag gagagaccca gaaggatata agaaagctct tgaagagttt
    32821 gatagtagac ttcctgaaat aatggctaat atgagagaag atgatatttt aataatcaat
    32881 gcagaccatg gaaatgaccc aacatataaa ggtacagacc atacaagaga atatatacct
    32941 gttatgattt atggcaataa gattaaaaaa ggttttaatt taggagtaaa agatactttt
    33001 gctgatattg gagcaactgt tgcagatata ctaaatgtaa agttgcctaa acatgggtca
    33061 agctttaaag aagatttatt ttaaactata aaatgataaa aacttagctt aataggatgg
    33121 agagattgga atgtttgaaa agatagcaca aagtagtaaa tttataaatt ctaaaagtga
    33181 tataaaacct aagattggtt tgatattagg ttctggacta ggtgatttag caaatgatat
    33241 agaagaccct gtcgcaatta aatatagtga aattccaaac ttccctgtat caacagtagc
    33301 aggacatgct ggacaacttg taattggaaa acttgaagga aaagaagtta ttgctatgca
    33361 aggtagattt cattactatg aaggatattc tcagaaagaa gctacttttc cagtaagggt
    33421 tatgaaagaa ttaggagtcg agattcttat agtaactaac gctgctggag gcgtaaataa
    33481 agaatttaaa gcaggagatt tgatgcttat aagagaccat ataaacttta gtggaagtaa
    33541 tccattagta ggaaaaaatg atgacagatt tggtgctaga tttccagata tgtcagatgc
    33601 atattcttct aaatatgtag atgtagttaa aaactgcgct aaagaatgca atatagatgt
    33661 caaagaaggc gtttatatgt tttttagtgg tccaaactat gaaacaccag cagaagtgag
    33721 aatggcacaa attttgggag ctgatgctgt aggtatgtct actgttccag aagtcatagt
    33781 agcttctcac tctaatatag gagttattgg tatatcttgt ataacaaata tggctgctgg
    33841 catcttaaat caaccactaa gtcatgagga agtaatagaa actactcaaa aagttaaatc
    33901 agaatttatg aatttagtta aaacaacggt taaaaattta taagttaaag cttattatag
    33961 attttttgag gaagtttata ataaaatagt taatgtttaa tttgaaaaga attaataaaa
    34021 agctgtcttg atatagaaag ttatttctaa taaaggacag tttttttata atactacaac
    34081 atttgaaagt gttttaaagt ttaatattta tttaaattat tataggttag aatggagatt
    34141 tcaattatga gaatgtatga tattataaaa aaaaagagag ataacctaga gcttagtaaa
    34201 aaagagataa actactttat agaaaactat tcaaagggag atatacctga ctatcaagct
    34261 tctgcactat taatgtcgat ttatttaaat aagatgaata aacaggaaac tgtacatctt
    34321 acagaagcaa tgatgaactc tggagacatg ataaacctat ctgagataga tggaataaag
    34381 gtagataagc atagtactgg tggtgttgga gataagacta ccatagctct tatacctttg
    34441 gttgcttcat gtggagcacc agttgcaaag atgtcaggta gaggtcttgg tcatactggt
    34501 ggtactattg ataaattgga gtctatacca ggtttttcga cagaaatgga gataagtaaa
    34561 tttattgatt ctgtaaataa gtctaaaata gcagtttgtg gtcaaagtgc aaaggtagca
    34621 attgcagata agaaaatata tgctcttaga gatgttacag caactgttga taatatatca
    34681 ttgatatcat caagtgtaat gtctaagaag ttagcttcag gagctgatgc aattgtgcta
    34741 gatgtaaaaa ctggtgatgg agcatttatg aagacattag aagagtcttt tgagttagct
    34801 aaatctatgg ttgatatagg tactggtatg aatagagata caatagggat tgtaacagat
    34861 atggatgaac ctttaggttt tgcagtggga aactctttag aagttataga agcaattgag
    34921 acattaaaag gaaatggacc taaagacctt gttgagctat gcgaagtatt aggagcatat
    34981 atgttggttt tatcaaaggt agcacatgat tttgaagatg gtgttgaaaa aataagagaa
    35041 tcaataaaat ctggtactgc tatacaaaaa ctcaaagagt ttatagagaa tcaaggtggt
    35101 aataaagatg tagtagatga ttattcttta ttcaaaaaat caaagtataa atatccagtt
    35161 aaatcaacta aatcaggtta tataagcaaa attaaagctg aagatgttgg tgtatcggct
    35221 atgattcttg gagcaggtag agaaactaaa gatgatattt tagatttatc agcagggata
    35281 attttagaaa agaaagtagg agattatgta aatgaaggtg aagttttagc ttacatgtat
    35341 tataatgatg aacaaaagct ttcattggca aagggaagat ttattgattc ctactctatt
    35401 gtagattcaa aaatagaaaa aaataaatta atatatggta ttgtaactaa agaagagatt
    35461 aaaaagtttt agaaaatttt agaaaagtga ttataaaagg cctaacatgt caaaaataat
    35521 gttaggtttt ttattttttg atagactata aattatacca aaaatatata ttttaaaaca
    35581 ttttttacaa tataaataag tttattctac aagatgattg aaaattaaag tatctagttt
    35641 tataatagaa aatggataaa tatacataag gagtagatta tattatgaac tttaaagaaa
    35701 atagattgaa aatagcagta ttaataattg ttattttgat tatcttagca ggtatatttg
    35761 tgtttataca aataggtcct tatgataaaa ataacaaaaa agatgtaatt atcgacgttc
    35821 caagtggagc atctgttgga aaaatatctg acatattata tgaaaataaa ttaataaaga
    35881 atgaactatt atttaaactt ttggtaaagg ttagcaataa agctccgtct ataaaatcag
    35941 gaacatactt acttaatcaa tcgtattcaa acaatgatat aataagtctt ttagtttcag
    36001 gtaaaatata tcaggatggt ataaaagtga ctattccaga gggagctaca tctaaagaaa
    36061 ttatagcaat gttagtaagt aaaaatttag gagataaagc tacttttgaa aatttaataa
    36121 aaaaacctca agaattttat gataaatttc catatttgaa ggaagatgga ataacctctt
    36181 tagaagggtt cttgtatcca gaaacttatt attttaattc aaagaaacaa tcagaagaag
    36241 atattttaag cgaaatgtta aaagtatttg acagtaaata cacggacaag tttaaaaaga
    36301 aacaaaaaga attaaatatg actcttcaag aagtaatgga aatggcatca ataattgaga
    36361 aagaagctgt tcttgacaaa gacagaccta taatagcaag tgtgttttat aatagactta
    36421 aggtaggtat gcctcttcaa tcagatgcta caatacaata tatatttgaa gagagaaaga
    36481 aaattgtaac atatgatgat ttaaaaattg attcaccata taatagttat aaaaataagg
    36541 gattacctcc tacaccaata tcaaatcctg gtataaaatc tattgaagca gcattatatc
    36601 cagagaaaac agactattta tattttgtag ctaagattga tggtggaaat aattatagta
    36661 caaactatca agaccattta aaatacgtta aagaatataa agaagcaagg gataaacaat
    36721 caaaagatac aaaagcaaca aataaagaaa atacaaaaaa ataaaccatg attataaaaa
    36781 gtctaacagg gtaagaataa tgttgggctt ttttattgaa aattgttatt cttagattta
    36841 taataaagaa agtgtaaata tatataacaa tatgaattta aaaaataata aattaatata
    36901 aaacttgaat atttattatt ttagggagtg tttttatgag taatatagtt aatgatttgg
    36961 ttgaagatta tataagagtg acattaaaag aaaaagaagg atttttaaaa gatttagaaa
    37021 tttatgcaga ggaaaatagt gttcctataa tacataaaga agtttcagat ttattaaaag
    37081 tcttgttaaa ggttcaaaaa ccaaagagaa ttttagaagt aggatgtgca attggttatt
    37141 cttctatttt ttttgcaact attataggaa aagatgcaga tataataact gttgagagaa
    37201 atgaaaaaat gatagaaaaa gcaaaagaaa acataaaatt agctggattt gacaataaca
    37261 taactatact agaaggagat gcagaagaaa aacttagtca agtccaaggt gaatttgacc
    37321 ttatatttat agatgcagcc aaaggtcaat ataagctatt ttttgattta gttatagata
    37381 aattaaaaga tgggggactt ttagtatctg ataatatttt gtataaaggt atggttgctc
    37441 atgatgattt tgttgtaaga agaaaaaaga ctatagttaa aagaatgaga aattatttag
    37501 attatatatg taattgtgac tatttaagca catcattggt accaataggt gatggtgtag
    37561 cactaagtta taaagaaagt aaaagaggtg atgtagatgg aattaaataa agtagaactt
    37621 ttagctccag ctggtgattt ggagagattg aaaatagcta taacatatgg agcagatgcc
    37681 gtttatatag gtggagaaat ttttggaatg aggtctgcag cgaagaattt tagtaaggaa
    37741 gatatggcag agggagtagc ttttgcacat gaaagaggca agaaagtatt cgtaacagta
    37801 aatataatac ctcataatga agattttcta caattagaag actatttgtt ggaactagaa
    37861 gagataggaa tagatgcagt tataatagct gacccaggag tgcttagtgt cattaaaaaa
    37921 gtaattccga atatggaaat acatttaagt acacaagcaa atacaaccaa ttatctaagt
    37981 gcaaacttct ggtacgagca tggaataaaa agagttgtag tggcaagaga attatctttt
    38041 gatgaaatat ctgagataag agcaaaaaca cctttagata tggatataga agcgtttatg
    38101 catggtgcta tgtgtatttc ttattctggc agatgtttaa taagcaacta tatgactgga
    38161 agagatgcaa acaaaggttc ttgtgctcaa tcttgtagat ggcaatatca tttggtggaa
    38221 gaaaaaagac ctggtgagta ttttccaata tacgaagatg aaaggggtac attcttcttt
    38281 aactcaaaag atttatgtat gatagagtat ataccagagt taatcaaatc tggtataaca
    38341 agtttaaaaa tagaaggtag aatgaagact gcatactatg tagctactgt tgtaagagca
    38401 tacagaatgg caatagatga attttacaga gacccagaaa actggaagtt caatccaatg
    38461 tggatggaag aactaaaaaa aggtagtcat agacatttta catcaggttt ttacttgaat
    38521 aaaccaacaa cagaagacca aaattatcaa tcagcatcat atgttagaaa ctatgatttt
    38581 ataggtatag ttagagaaac agaagatgaa gatggtctta ttgttgttga gcaaagaaat
    38641 aaaatgtgtg ttggtgatga aatagaagtt atgggaccat ataaagaaac tatgtttact
    38701 aaaattgaag aaatgtacaa tgaagaagga gaagctatag aatcagctcc tcatcctaga
    38761 caaatagtga agttaaagct atcagtaaaa gttggtaaag attatatgct tagaaaagtt
    38821 atagaagaaa aggttgaaga ataataaaaa attgataaaa atagtattag ccccttagaa
    38881 attgtcaata atgaaattat ctaaggggtg atttcatgtc aaaaaagaag actccatttt
    38941 tgaaaaaagt gggaaagaga agctggtgta tatttactat aattttaata atttatagtg
    39001 ttttaattta taggctagtt gatatacaag tattaaaagg tgataaatac aaacaaagtg
    39061 ttgaatcaca aagcgttgag aaagtcgagt taaatagtgg aagaggaata atctatgata
    39121 gaaacaacaa gaagcttaca gatacaagta aatctcaggt attaattgtt gaaaaagaaa
    39181 aattaaataa taactataaa attttggagc ttattaagaa agctactaag atgaatgatt
    39241 tagatatata taaagctgta caagagcaat tgacacgccc tataatacag attcagacta
    39301 aaaatatcga taagagtatg aaaaaagaac ttgagaaaaa tggaataatg gtagaagaaa
    39361 aaactatgag gtacgcaaaa gatggtttat tatcacatac aataggatat ataaaagaag
    39421 atgataaatc aggtcaatca ggaattgaaa agagtatgga tagtgtactt agaaattcaa
    39481 atgaaaagta tataagtgct tttaaagctg gagatgcagg aaatgaaaaa tctctaaaca
    39541 tactaaaggg aagtgtaaaa actgttgata ataaggataa agatagacat ctaaaaacta
    39601 ctattgatta taacattcaa aaaaaattag aacaaatact aaataaagaa gaaaatccta
    39661 cagcagcaat aatctcggaa gcatctacag gtgaaattct tgcaatgtgc tctagaccta
    39721 attttgacca aaatgacata tcaaaaagtt taaaaggaaa aaatggagaa tttgaaaata
    39781 gagttataaa agcgacatat ccaccagggt ctgtatttaa gatggtagtt ttattttctg
    39841 cacttgaaaa tggtgttata gatgagaact atacttacaa ctgtacaggt aagactaaag
    39901 ttgggaatac taatgaaata ctgcgatgta ataaaagaga tggtcatggt ttccaaaact
    39961 taagacaagc tttttctaat tcatgcaatc ctgcattttt agatatagct atgaaacttg
    40021 gaaaagaaaa gatactcaaa tctgctgaaa aattacattt atttgaaaag gttgatattg
    40081 gtcttgatga agaaaaaatt agagaagctc caaaaaacat atcaataaga aatttagcta
    40141 taggtcaaga aaatatagaa tttacaccac ttcaaataaa tcaaatgact caaataatag
    40201 ctaataatgg aacattcaaa ccactatact tatataaaag tcttgtggac aataatatga
    40261 atacaataaa aacttataag tcttcaaaaa aagaagaact tatttctcct tatgtgtgta
    40321 cacaagttaa agaatatatg aagtctgtat ctaggattgg tacagctaaa gacctaaaag
    40381 atattgaagg tggttgtgga gttaaaactg gtacagctca aagtagttta aataaaaaag
    40441 ctatagacca tggatggata acaggatttt atcctgaaga aagacctaag tatgtaataa
    40501 ctgtgttagt agaaggaact caaaaaggaa ataaatctgc aacacctata tttaaggaaa
    40561 tatgtgaaag tataaaataa attatgtact ataaaattta aggatatatc gtaactttga
    40621 ttttagatag aaaattttgt ctaatgatgt acgatatatc cttttaaaat atagtattaa
    40681 ctttcttgaa caagctcttt ttctagtttt ttaagagctt ttttttcgat tctagataca
    40741 tatgaccttg atatatcgag tttttgtgca atttctcttt gtgtcttata gccataagga
    40801 gttaacccat atcttaactg gacaatttct ctttctctag gagtaagaat tttgtttagt
    40861 tgttcgtaaa gttttccaac ttgaactttt agttcaactt catcaagaac atagttagcc
    40921 tctgtaccaa gtatatctaa aagacttatt tcattacctt ctttatctgt tccgataggg
    40981 tcttgtagag atacttgagt tttatttttt ttgttagatc taatattcat taaaatctca
    41041 ttttcaatac atctagaagc atatgttgct aatcgagttc ctttatctat gttataagtt
    41101 tctatagctt taattaatcc tattgtacct atggatatta aatcatcctg atcttccgtt
    41161 gaattattat atttctttgc tatataagct actaatctca tgtttctttc aattaatgta
    41221 tcttttgcac ttttatcgtt ttctatttta aatttagtta aatactcaat ctcttcttca
    41281 ggggttaagg gtttttcaaa agatttaaga gctgccaata ggtcaccccc ttttcaacgg
    41341 tcactttaaa ttatatgaag gagacttatc caagttgact gttatctaat aaaataatat
    41401 gcatattttt agaatttaaa acatatattt gcaaactttt gaatggaata tgacataaag
    41461 agtgggaatt taaatttaag tttttgttgt atatatatga aacaaggcga agaaattatt
    41521 gatttataaa gtataaattg gaattgaggt gtcaataaat ataaaaggaa atagtattta
    41581 tatatgtttt ggtgttgaag taaaagatta atgcaaagca ttagagtcta taaaaatatg
    41641 aatataggag gtgtaatttt acaatactta aaaaagtaaa tgtaattaat tgtttgtata
    41701 aagttgatta caagcaaaag tatcagtcat agttaatact gttaaggatg aaggtggtgt
    41761 gttaatttat atatgtatgg gaagaaattt tgattacaat gaaaaaactt acaaaagata
    41821 tttacgaaaa aacttatagt taaatgttta tatataaaat attatttagg ggttgttttt
    41881 attattgaaa ataagaaaga gtggttttat atcaattgaa tgtataataa gtattgctat
    41941 attatatgtg gctgtttatt tagtttctac atcattgtat aattgttata gttttatcag
    42001 tagaaatata tctgacagag aaatgttaag tacagcaaaa aaatatatag aagatgagaa
    42061 gtatagaata caaaatagta agtatgagtt aattgaagat aagatagaaa aaaattacat
    42121 aaatggatat gaaattaaca gtagaataga gcaaatttta gattattatc aatgctatga
    42181 aataaatata gagataaaaa atgaatttaa aaaactgagg tttaatagct atgttactag
    42241 aaaataaagg ctctatatta ataataacat tgataatatt ttctattata agtactgtgt
    42301 gcattatgtg tataggttta atttatacac ataataatat gtttgagtta ggatacaaag
    42361 acattcaact aaaagaaatt tctttaagtg gaatagaaat atcagtaagt aatgtacttt
    42421 cttgtgttga ggaagctatt gatatatcta ataatgaaga cgaatttaga gaatatttct
    42481 taggaaataa tatgattgaa tgtataaaaa aaataaaaga tacatcttat agtagtttag
    42541 aagaactttc tttaaaagta gaaaataata ctatttatga cagaggagat ttttttgaat
    42601 ttgatataga aagtaatgtt agagaaaaag aatttttaaa agcaataaaa gtaagagtga
    42661 aaatcaaaaa tccatggata ggaattgata ttgaagatta tatggataaa tctaataaca
    42721 ataaaattga tgacaaaaca ttcaataatt ataaagcatc tgaaaataat gaaattataa
    42781 atcaatttaa aaatgaaagt gaaaatataa ggaatcaaga ggttgttgaa tttacagaag
    42841 aagatgaaga agaacttaaa caaaacataa ttgaaaatat aaagaagaat tttgatgaaa
    42901 aaaacctagt acaggtatat aagtataggg gtgtataact ttgaaaaaag aaggaatgtg
    42961 actgtaatag aattaatagt atctatcagt ataatactct tgatagttac attatctttt
    43021 ccaaaagata atcttgaaaa tcataaaata aatttatttg cacgtcaact ttgttcagat
    43081 ataaggtatg ttaggcgaat taatatgttt agcaactaca atacatatat atattatata
    43141 aatcaaggta aacacgaagg ttatgtattg agaaaagatg gtaaaaacat taaatcaata
    43201 atgctaccta aaaatgcaaa aatagctagg agtattgaaa taataaagtt taaatctgat
    43261 gggtcgcctc aaaaggcgtc tacaatagag gtatataata atagattaag aaaaactata
    43321 acaataacac ctgtaagtgg aagagtactt ctgaaagagg gaaaatatga aacataaata
    43381 tggttacttg cttttggaaa gtgtcgtatc attgtcttca atggtgataa ttatactagt
    43441 tttatattct atatttttgt ctacaataaa cttaaagtta aaagtagaag ataagataga
    43501 actacaacaa caaagtcttg aaatcataaa atctatggaa ggaattatta gcaattctat
    43561 gggaataatg aatgtatcta attatgaaga aacatttaaa aagacaacat ctataaaatg
    43621 tagatattta gatgaaaata ataatgaaga aggcataagt aataaagaaa ttatattgaa
    43681 tgaaagacga aataagttat ttgtaaattc tttaaatggc gaaagtagtc aagcaggtgg
    43741 ttatgaaatt ggagattatg tagatgaaat gtatgtatca attactaata atggtcaata
    43801 cgttaatatt aaattgaaat tatcaaaatg tagtcaaaaa tatgagactg atttcaaaat
    43861 aaaagtatgg aattttagtg aaagtatata aaaatacata aatttaagag aaagaaatga
    43921 catcaataag tcatataaaa tataattatt gagaactata taactaaata atatcaatat
    43981 gtaaagtgta tagattaaga atatagagga taataataaa aatatagaat aagtaatttg
    44041 ccaaatactt gtttatattg tataacttat gatattatga tattagtaaa aatttgtaat
    44101 cctgacttga cagaattcaa gtaagagata taattttaaa ggcatagtaa tgtaaaattt
    44161 actaaaaaat aagaaattta ttttaatatg gaggaacaaa aaatggtatc agcaggtgat
    44221 tttagaaaag gtgttacatt tgaaaaagat ggacaaccat gtttagtagt tgattttcaa
    44281 cacgttaagc caggtaaagg agctgctttc gttagaacta aatacaaaaa cttaaaaaca
    44341 ggagctataa gagaagaaag tttcaatcca agtgaaaaat tccctaaagc tgttatagat
    44401 acaagacaaa tgcaatatct atacaatgat ggtgagttat attattttat ggatcaagaa
    44461 aattttgaac aaataccatt aaactatgag caagttgaag atgcaattaa gttcttaaaa
    44521 gaaaatgaag ttgctacaat aagattttac caaggacaac cattccaagt ggaagcgcca
    44581 aactttgcag agctagaagt tacagataca gagccaggca taaaaggtga tacagcaagt
    44641 aatgtaacta aagcagctac agttgaaact ggagcagttg ttcaagttcc attatttata
    44701 aatactggag ataaagtaaa gatagatact agaacaggtg aatatttatc aagagtataa
    44761 aaatttatgt atataaaagc ttatatgtct taataatata agatatatga gcttttagtt
    44821 tatatataaa aaagaaagcg aggggtgtta catgaaacat ttatatgaag aagttgcata
    44881 cttaaaaggc ctagcagaag gactagaaat aagtgctgaa agtaaagaag ggaaaatgat
    44941 acataaaata gttgatgcat tagaagtatt tgcagatgct attgtaacat tggatgaaga
    45001 acaagaagaa cttcaagatt ttgttgaatc tatagatgaa gatttagcag atttagaaga
    45061 agatattgct gacatggaag aagatcttta tgaagaagat gacgatgagg atgatgagga
    45121 cttcagctat atagaaatgg aatgcccaaa ttgtggagag ttagtagaaa tagacgaaga
    45181 tttgttatat gatgatgaag tagatgttgt ttgtcctgat tgtaaggctg tgatattatc
    45241 atcagaagat gattgtgatg atgattgtac atgtggtggt tgttcaagtt gtgatgatag
    45301 agagtaaaaa ttcataagaa ttaaaactgc ctatgatgcc tatgagatat tttgcttcat
    45361 aggtagtttt atttttatga ggaagggtat tatgtatttt ggttttgcaa tacttggaca
    45421 aaaatagtac actagaattt atagaatagg attttggagg gatgtaaata tgaaaataag
    45481 taaaaaatta ctagataata tacaaagatt tgaaaatgtt ataaattata aatttaaaaa
    45541 taaagaatat atactagaag ctcttactca tagttcatat tctaatgaaa ataaaaaata
    45601 taattttaac gaaagattgg aatttttagg ggattcggtt ttaggcatag tgataagtga
    45661 ttatttattt aatgaagaag ctaatctgcc cgaaggggaa ctaactaaac ttagagcaaa
    45721 tatagtctgt gaagattcat taagtgaagt agctaatgat ataaatttag gtattcacat
    45781 gttattaggt agaggagaag aagcaacagg aggaagacat aggacttcaa tattagcaga
    45841 cgcatttgaa gctgtaatag ctgctatata tcttgatgga ggttttgaaa gtgcaagaca
    45901 gtttatactt catcatatgg aaaatataat ttatgattca agaaaaggaa atatatttag
    45961 agactacaaa acgcatcttc aagaggtgtt acaagggaat ggagaaaata atatctggta
    46021 caggcttata gaagaaaaag gaccagacca taataaaaga tttgtaatgg aagttggaat
    46081 taatgacgat gtacttggta taggagaagg caagagtaaa aaagaagctg aacagttagc
    46141 tgctaaaata gctttaaaga aaaaattatg ggaaaaataa atataatatg taaaaatata
    46201 aaaagaaata tttatattag aaggatgttg tagatgaaaa agagaattat acctatattt
    46261 gttcctcaca aaggatgtcc tcatgattgc atattttgta atcaaaaaaa aataacaggt
    46321 gtgagtactg atgttactag tgaagatgca agaaatataa ttgaggaatg tttagagact
    46381 atagataaag atgcagatgt agaaatagca ttttttggtg gtagctttac tgctatagat
    46441 attgatatac aaaaaagttt attatcagtt gctaaagaat atgtagaaaa aggtcttgta
    46501 aaagatatta gaatgtctac aagaccagat tgtattgatg aagatatatt aagtatgtta
    46561 aaagaatata agacaagtat aattgaacta ggagtacaat ccttagatga aaaagtatta
    46621 ttagatagta taagaggtca tcaatctgaa attgtataca agagttcaaa gatgataaaa
    46681 aatagtggaa tcaaacttgg attacagatg atgattggtc ttccagctga cacagaagaa
    46741 aagtgtatat ttacagcaaa aaaatttgta gagctaaaac cagattgtgt tagagtctat
    46801 ccaactttgg tagtaaaaga tacaggtctt gaaaagttaa tggaacaaaa taaatataat
    46861 ccatttaccc ttgaagagag tgtaaatata gtaaaaaaag ttcttgtact gttttatgtt
    46921 aataatatca atgtaataag agttgggctt caagctactg atgatataca aattggaaaa
    46981 gctgttttag caggaccata tcatcctgca tttagagagt tggtagaagc agatatgata
    47041 aaagattatt tagaatttgt tattttgcaa aataaaaata taaagcaaat gttggtcaaa
    47101 tctaataaaa agaatatttc taaaatcata ggaaataaaa aaacaaatgt taaatatatg
    47161 gaagaaaaat ttggtgtgct tttaaaaaca caagaatcag atttagatat aaatcaatta
    47221 gaaattgtgt tagatgggaa aagtttaatt agtgctaata tgagagacat tcatagaaaa
    47281 ttgtatgaca tttatgacct ttagttttaa taatgaaggg agagaaaaga tttgtattta
    47341 aaaagactag agttaaaggg atttaaatcc tttccagtaa aaacagatat tatttttaaa
    47401 gaaggaataa cagccatagt aggaccaaat gggagtggaa aaagtaatat atctgatgct
    47461 gtaagatggg tattaggaga acagagtatt aagagtctta gaggtgataa actcgaagat
    47521 gtaatatttg caggtacaga tacaaaaaaa cctatgaatt attgtgaagt ggctcttact
    47581 attgataatt ctgaaaatca attggaatta gattttacag aagtaactat aagaagaagg
    47641 gcttatagaa atggtgaaag tgaatttttt ctaaataata aaagttgtag attaaaagat
    47701 ataaaagaag tctttttaga tactggtatt ggaaaagatg gttattcaat catagaacaa
    47761 ggaaaagtag atgaaatatt aagtaataat cctttaagca gaagaaaagt ttttgatgaa
    47821 gcttgtggca tatcaaaata tagatataaa aaacaggaag cagaaaggaa tttaagtaat
    47881 acaaaggaaa atttagaaag aatagatgat gtatatatag aaattgaaaa tcaattaaag
    47941 cctcttttta atcaacagac aaaggcaaaa aagtacttgg aaataagtga aaaattaaag
    48001 acacttgaag taaatagctt tataagagaa attgaaggaa tagaaaaaga attaagtgag
    48061 gttaatgaac atcgtaaggt tattgaaaag gagctcaatg aaaaagaaga acaaaaaaat
    48121 gttgtagaga aaaagcaaga agatattaat aaagaggtag aggtattaca agatgtaatt
    48181 gaaaaatctg tagattatat aaattcaatt aaaggagtta tatcaaaaaa agagtcccaa
    48241 ataaatttaa ttaaggaaag aataagaaat tttacgaatg aaattagtag aaaaaattta
    48301 gaaataaatg atataaaaga gaagctaaat gaaaataagc aatacataaa agaattagaa
    48361 agcaataaat tatcaggtag tgaagaacta tctaccttgc aggaaaatat aaaagtattg
    48421 gaagcaagta aagataaaca aaaaattaag ttagaatccc taaataatga gatagaatta
    48481 ttgaaagaaa gtataattga tatattaaat aaaaaacaag agtttagtaa taagctgtcc
    48541 actttaaacg caaataaaga aaatatgaat atcagagatg aaaatataaa ttcagagata
    48601 acagagttaa acaaaaatat tgaaataaaa tcatctgaat tagatactat aaataaagaa
    48661 tttaatatgc aaaatgaaaa tttaaagaat gtaaataata gacataaaga attatcaact
    48721 aatttgcagg attcaattag tgaacacaat aaactagaag atgaaataca gaaaagtaaa
    48781 tataatttaa atggatataa ttcaaagtta aatgtctata tagatatgga aaatcattat
    48841 gaaggattta atagaggagt aaaagaagta ctaaaaaata aaaatttaaa aggagttcat
    48901 ggagcattag gacagattat aaatgtacct gaaaaatatg agaaatctat agaagcagct
    48961 cttggtgctt atatgcaaaa tataataact gataatgagt ttagtgctaa gtctgcaata
    49021 aactatctaa aacagaataa tttaggtaga gtaacatttc ttccattaaa tataattaaa
    49081 tcaaataaaa taagtctagg aaatttaaaa gcaaatacta agttcatagg aatagcaagt
    49141 gatttaatta catttgatga aaagtataga aatataattg aaaatatact tggaagaaca
    49201 atacttataa ataatataga tgagggcata aaatttgcaa aagaaactgg tcataggttt
    49261 aaaatagtaa ctttagatgg tgaaatctta aatccaggag gttctttgac tgggggaagt
    49321 ctaaaaacta atgggaatat actatccaga aaaagataca ttaatgaata tacagaaaag
    49381 ataagtaata tcaagaatga aatttcacat ctagaattaa aaagagaatc tttagacaaa
    49441 gatgtgaaaa atatcaaaaa tgaaattgat tcacatgaaa gtaaaataaa agatttagaa
    49501 aaaagtataa taataaaaag tacaagtata aagaatgttg agtctgaaat tgaatcgtta
    49561 aaaggtagta ttactaaatt agaaaatgag aagaatgatt taaattctaa cctaaattat
    49621 acacttgaaa aaagtgatga tgttagaaaa gatatggaag agttagatga tttatacaat
    49681 aaaaataaag aaaaaattga tgcattgaat gaggaaataa aaagatacaa tgatttatat
    49741 gataaagaaa aatctgaatt tgatgaattg aacttaagtt tagtgaagaa aacagaagtt
    49801 tacaatagta tagttagaga tataaaaaga atatctggtg aaaattgtga gctagaagaa
    49861 aaaaataaac aattagaaga gtcattaaat tatgaagaac atgaaataat taaattacaa
    49921 gattctatac tcactgaaga aaaagaaaaa gaaaatttaa caaaacaact aggggatagt
    49981 aatagaaatc ttgaaactag aaaaatagca aaagatgatt taaaaaatag ttttgatgaa
    50041 attaataaag aattaaaaac tatagataga cagcatatag aacttaaaga aagtttattt
    50101 aaagttggtg gaagattaga aagattaaaa actagtcaag acacatatat aaataagttg
    50161 tttgagcaat atgatatgac cttagtacaa gcattggaaa ttaaagatga agatttggat
    50221 atagatagaa aattcttaga aagtctaaag agagaaataa gaagtcttgg aaatataaat
    50281 atagactcta taaaggaata tgaggaaata aaagaaagat acgattttta tagcgaacaa
    50341 aagcaagatt tagaagagtc tatggaagag atagaaaaac ttatacatac tttagaagaa
    50401 aatatgaaat ctgaatttga aattaagttt gaagaaataa gtaaaaattt taaatatgta
    50461 tataagagat tatttggtgg aggttgtgga gaattaacta ttttagataa agaaaattta
    50521 ttagaaagtg atatactaat aacagctcaa cccccaggta aaaaaatgaa aaatttaaac
    50581 ctattatctg gtggagagaa agctttaacg gcaataagta tattatttgc tatattgatt
    50641 acaaagccaa caccattttg tatattagat gagattgaag cgccacttga tgacgcaaat
    50701 atatttagat ttggagaatt tttgaaggat ttatctaaag agactcaatt tatatcagta
    50761 actcatagga gaggaactat ggaagctgct gattatatct acggagttac aatgcaagaa
    50821 aaagccattt caaaagttat tagtttaaaa ttaaaagaag ctcaggaaat aacagatatt
    50881 atataactta gattgacata ggaggaataa cagtgttaaa aaaattattt ggttttggaa
    50941 aagacaagga aaaggaaata gaaaaaaaag atgctgaaga agaaatagaa gttgaagaca
    51001 gtgttgataa tttagaaaac ttggaagaaa ctattttttc aggacttgaa gaagaagtga
    51061 tagataaagt ggaagatgtt gaagaaaaaa atgaggaaag tgtcaataat gaaggcactg
    51121 aagaaatcga aaactttgaa aaaatagaag ttgagatgga tagtaatgga aaagaagtag
    51181 aaaatatttc aaataatgat tctcaagaag agataatcga agaattagaa aattatgatg
    51241 aaattgagtc taaagagata gaagataaag aagataagaa agttaacttg tttgaaagac
    51301 taaaacaagg attgacaaaa gcaaagcaag gaataactga tagaatagat gaagttttaa
    51361 aatcatatac aaagatagat gaagaattac ttgaagattt agaggaaatt ttaataacag
    51421 cagatgttgg tgtaaataca actatggata taattgaaag actaagagat aaaataaagc
    51481 aaaaaggtat tactgaacca ataaaggtaa gagaagaact taaatctata gttgaagata
    51541 ttcttacaaa tgaaaactct acactagata tagaaccagt accatgtata atattaatgg
    51601 tgggtgtaaa tggggtcggt aagactacta caataggtaa actagcaaat agatacaaaa
    51661 aagatggtaa aaaagtttta ttagcagcag cagatacatt tagagcagca gcaacagagc
    51721 aattagaaat ttgggcaaat agaaccaatg tagacataat aaaacatcaa gagggagcag
    51781 accctggagc tgtagtattt gatgctataa aagctgcaaa agctagaaaa acagatgtat
    51841 taatatgtga tacagcagga agattacaca ataaggctaa tcttatgaat gaacttggaa
    51901 aagtatttaa aatagtagat agagaatttc cagaagcaaa acgagaagta cttcttgtgg
    51961 tggatgctac aacaggtcaa aatgcagttg ttcaagcaaa gacatttaaa gaagttgcag
    52021 atataacagg tatagtactt acaaaacttg atggaactgc aaaaggtgga gtagtccttg
    52081 cggtaaaatc agaagtagat gtacctgtaa aacttattgg agttggagaa agtgtagaag
    52141 acctacaaga tttcaatgca aaatcatttt cagatgcatt atttggtaac taatgttctt
    52201 ttaaaaataa tggttgacaa gagtgttgtt atgatataaa atacaatagt gtaaagtaaa
    52261 taacctttac agtatactgc taggaagtgt aagatatgaa tatagaaaaa atggtcgaaa
    52321 ttggattgtt atttgaacag tataaagaat tattaactga caagcagaaa gaaatagttg
    52381 ctctatacta tgaagaagat tattctttag gtgaaattag tgaaaatctg aatgtatcaa
    52441 gacaaggtgt ttatgatacc ttaaagcgtt cagaaaaaat actaagagat tatgaagaaa
    52501 agttacactt agtatcaaag attcaagagc aggaaaaaaa tattaagatt ataaaagata
    52561 aaattattga cattaaagaa gatttattgc acaatagaga ttgtgctaat ttaatcccta
    52621 agctagaaaa tatagaagat gtatgtaggg agatgataaa atgatatttg aaggattgtc
    52681 tgataaacta caaggggcat ttagtaagtt aaaatctaaa ggaaaactta cagaagtaga
    52741 tgtaaaaaat gctatgagag aagttaaact tgctcttctt gaggcagatg ttaactttaa
    52801 agtagttaaa gatttcataa aaaaagttca agagagatgt gttgggcaag aagttatgca
    52861 aagcttaact ccagctcagc atgtaatcaa aattgtaaat gaagagctta caagtttaat
    52921 gggagatgta caaagtaaag taatgatttc ctcaaagcct cctacaattt taatgatggt
    52981 tggattgcaa ggtgcaggta agactactac agcaggaaaa cttggaggat attttaaaaa
    53041 gcaaggtaaa aaacctctat tagtagcttg tgatatttat agaccagcag ctataaagca
    53101 acttcaagtt gttggagaaa aattggatat acctgtattc aatatgggag ataaagaaaa
    53161 tccagtaaat attgcgaaag caggtttaag ccatgcgatt aaaaatgcta atgatttagt
    53221 tattatagat acagctggta gacttcatat agatgaagta cttatggatg agttaaagtc
    53281 tattaaatca gaagttaagc cacatgagat acttttggtt gtagattcta tgacaggtca
    53341 agatgctgta aatgttgcag aaagcttcaa tgaggcactt ggtgttgatg gagtagtatt
    53401 aactaaattg gatggagata ctagaggagg tgctgcactt tcaataagag cagttacaca
    53461 aaagcctata aaatttatgg ggatgggaga aaagctagat gatatagaac ctttccaccc
    53521 agatagaatg gcatcaagaa tattaggtat gggagatgtc ttaagtttaa tagaaaaagc
    53581 acaagaaaat atagatttag agaaagctaa agaattagag caaaaaatta aaaaacaaga
    53641 gcttgatttt gaagatttct tacaacaaat ggaacaaata caaaatatgg gacctcttga
    53701 caagatactt ggtatgatac ctggtatggg aaatgtaaaa gaccaacttg gagatgtaga
    53761 tttgaatggc aaagaaatga agagaacaaa agccataatt cagtcaatga ctacatatga
    53821 gagaagaaac ccaggagtat taaatgcttc aagaaagaaa agaatagcta aaggcagtgg
    53881 aactagtgta caagatgtaa atagacttat aaaacaactt aatgaaatga aaaaaatgat
    53941 gaagatgttt gctggttcac aaaagagtat gaagaaaagg ggcggcttag gcggtttacc
    54001 tttttttaaa taaaatataa aaaagtaaaa attattcgga ggtgactgta atggcagtta
    54061 aaataagatt aaaaagaatg ggagctaaca aaaaaccttt ctacagaata gtagtagctg
    54121 attcaagagc tccaagagat ggaaaattta tagaagaaat aggatactat aatccaattt
    54181 ctgaacctaa acaagtaaga ataaatgatg aaaaagctat aaaatggtta gctactggtg
    54241 ctcagccaac agaagtagtt aaaaaattac ttgtaaaaaa cggagtaata gaaaaattcg
    54301 aagcttctaa acaagcaaaa taattttttc ttggcaatta gtttaagtaa gggctcattg
    54361 ctaaggttac tttaggagat gaaatatatg aaagagctag ttgtagatat agctaaggct
    54421 ctagttgata atcctgactc agttgttgtt gaggaatttg aagataatga tggtatcgtc
    54481 ttaaagctta cagttgctca agaggatatg ggtaaggtta ttgggaagca gggaagaata
    54541 gctaaagcta taagaactgt cgtaagatct gttgcaaata gagaaaatat aaaagtttct
    54601 cttgagatag tataaaatag gaggattagg tataatgcct agtccttttt atataaatgc
    54661 gtagtttaag attttatgat gatatgacca cataagatta aattttttaa gaaggtgata
    54721 acatgaaaga aaaattaact cattttaagg taggtcaaat agtaaataca caaggtttaa
    54781 aaggtgaagt aagagtttat ccgcttactg atgatataga gaggtttgat gaattaaaag
    54841 atttctatct gggaaaagat ttaaataata aattggctgt tgaaaaagta agatacaaag
    54901 gttctatggt tataatgaag ataaaaggca tagacagcat agagaaagca gaaaaactaa
    54961 aaaataaatt tatgtatgta tctagagaag atagcagaga gcttgaagaa ggcgaatttt
    55021 ttatagcaga tatgattgga atggaagttt taactgtaga tggaaaatat gtaggaatac
    55081 tagaagatgt tcttcaatac tcagcaaatg atgtctatgt agtaaaaggt gaggaagata
    55141 aagaatttat gataccagct ataaaaaaat ttgtacctac aatagatatt gatgaaagaa
    55201 aaatgattat agaccctatt aagggaatga tagattaggt ggtaatatga gatttcatat
    55261 aatgacactt tttccagaaa tatttaattc gtacatggat gaaagtataa tgaagagagc
    55321 agttgaaaaa ggtataattg aggttcatat atataatata agggattttt ctaataataa
    55381 acataaaaaa gtagatgatt atccatttgg aggaggagcg gggatggtaa tgactcctca
    55441 acctatatat gacacatata agcatataat aacaactcat aatataaata acccaagtgt
    55501 aatttatctt actccaaaag gtaaggtata caatcaatct atggcaaagc agatgtcttt
    55561 aaaagaagat ataatactac tatgtggtca ttatgaagga atagatgaga gaatcattga
    55621 tttaatagtt actgatgaaa tttcaattgg agattatgta ttaactgggg gagagctccc
    55681 agcacttata atgatagatt ctatatcgag attaatacca ggtgtattaa accaagaaga
    55741 atcatttgaa gaagaatcat ttaaagacaa cttattggaa tatcctcatt acactagacc
    55801 tagagatttt gaaggattaa aagtaccaga agtgctttta tcagggaatc ataagaagat
    55861 agatgagtgg agaagagaag aatctataag gataaccaaa gaaagacgat ttgatttata
    55921 taaaaagtct aatgaaaaat aaaaatataa tgaaaaatta atatatatgt atacaataaa
    55981 gtacctataa atggaattaa aaacataaaa gtgcttgtaa taactattga agtatagtat
    56041 aatataaaat gttgaaaata cgggcgttcc tctgtttaca ctggtcgggt agttaagaac
    56101 gtctatgata tctaggagga agaaagatga atgaaatgct aagagcaatt gagcaagaac
    56161 aattaaaaaa tgaagttcct aattttggac ctggggacac agttaaagta cacgttagaa
    56221 ttatagaagg aaaaagagaa agaatacaag tattcgaagg tgtagtatta aagagacaag
    56281 gtggaggagc aagagaaact ttcacagtta gaaaaatgtc tttcaatgtt ggtgtagaaa
    56341 gaactttccc agttcactct ccaaaaatag aaaaaataga agttactaga aaaggtaaag
    56401 taagaagagc taaactaaac tacttaagag gtagagtagg taaagctgct aagataaaag
    56461 aagctagaaa taaataatta aaaactttac ttataaatgg ggggctgatt tatcagtcct
    56521 ctttctattt taatgaaaat tacaacaaat aattatacta gaaataaata gaaatatttg
    56581 ttgaaaatat agattattta tctatgaatt tatagctttt aaaatgaaaa ataaaattgg
    56641 taaatattgc attatatttt gtaacgatat taaaaataag agaacagtat tatgtaaagt
    56701 atgacgttaa aaagatgaca atattatagt ttgtgaagat atatttttta agtggaatta
    56761 ggatataata atataatatt aataaggagt gatacatgtg tcaaatgaat acgaagagta
    56821 tcttatggat gacaatctac atataaactg gtatccaggt catatgaaaa aaactaagga
    56881 gttagtaaaa aataacttaa aattgattga tgtagtaatt gaacttttgg atgctagaat
    56941 cccgtttagt agtaaaaatc cagacataga caggcttgta ggagataagc caagagttgt
    57001 cgtacttaat aagagtgata tggcagatag ggataaattg aatcaatgga ttgaatacta
    57061 taagaaaatt aatatcaaag ctataccagt agatacaata aaaggtgttg gtataaataa
    57121 gataatcgaa gaatgtaaaa atgtaactag agaaaaaatg agttctttaa aagacaaagg
    57181 gagaaaagaa agagctatac gaataatgat agttggcgtt ccaaatgttg gtaaatcgtc
    57241 tcttataaac aaattaacag gtagaaaaag cactcaaact ggagataagc caggagtaac
    57301 gaaagggaaa caatgggtta gattaaaggg aaatcttgaa cttttagata cgcctggaat
    57361 actttggcct aagtttgaag accaagaagt tgcattaaat ttagcattta gtagagctat
    57421 aaaggatgag attttagata ctgaaacttt ggctttaaga cttatagaaa aacttatgaa
    57481 aatagaacct gagaagttga aagccagata taagttagat tgtctaggag aaactccaat
    57541 tgaaactatg gatatgattg gtcataaaag aggatttata acaggtaaaa aagaactaga
    57601 ttatacacgc atagccacaa ctgtattaaa tgaatttaga gatggaaaaa taggaaatat
    57661 aacattggaa gttccagaaa acgttaaaag atagatttta aaacaaaatc aaaaatttaa
    57721 tgttaaaaat ttaaaaaagc cttttagcaa aggctttttt atttttttga aatatagaca
    57781 aatatctcca tataaaagta taaattaggt tagaaagcct ttaaaaatat taatttaaga
    57841 caaatatacc taatattttg aaaattaaaa tgagtgtgat tattaaaaat tttagcatat
    57901 tgacttgctt gtttcgtata atatataata agcacaaata aatacaatat attaaaaatt
    57961 taatttattt aattttatta atttaataat aagttcttat tgtttaattc aacttactta
    58021 tgcaagcgta aacaatcgag acattttatt aatttaataa ggttaaaaag ggggatttta
    58081 taatgggtaa gaccaaggat gttatagtat ttggatttgc gttatttgca atgttctttg
    58141 gagcaggtaa cttaatattt ccaccttact tgggaataat tacaggtcca gaatggttaa
    58201 ttgccttctt aggatttacg tttgctgatg ctggattggc acttctagca gtaatggcta
    58261 cagctaaatt cgatggaaac gtagttgaaa tgtttaaaag atgtggtatg aagttaggga
    58321 ttcttatagg ttgtgcagat attttatgta taggaccatt cttagctata ccaagaacag
    58381 gtgcaacaac ttatgaaatg ggtataatgc ctttatttgg aacttcaatt ccagtgttat
    58441 tattctgtat actttttttt gcgctttcat atgtactaac aataagacct tctaaggtcg
    58501 tagatatagt tggacagttt ttaactccag ctcttttaat agctttagca tttataatta
    58561 taaaaggtat tatatcacct cttggagata tagtagataa acctatgata cctaatgtat
    58621 ttgcagaagg tataggtcaa ggatatcaga caatggatgc ttttgcagca atagcacttg
    58681 catcagttct aatagtatct ttaaatgata aaggatattc tactattagt gacaagttaa
    58741 aaatgattgg taaggcgggt gttcttgctt gtggaggatt agctctagtt tatggaggat
    58801 tatgtttcct tggagcaact gtatcaacaa tgtatggaac agatgcagta caatcacaag
    58861 taatagtaaa tataactgaa ggattgcttg gaaatgttgg gaaagcgatt ttagcagtag
    58921 tagtatcatt agcatgttta actacatcta taggtcttac atctgccaca ggtcaatatt
    58981 tctctagact tacaaaaggt aagttgagtt atgaaaaaat agtattagct gtatctgtat
    59041 tcagtgcagt agtagcatct tttggtgttg gaactataat aaagatagca tctcctatat
    59101 taagtatagt ttatccacca tcaattgttt taataatttt agctttcttc aatgaaaaaa
    59161 ttaagaatga taatgtgtac aaaggagcag tatatatgtc attgcttgta agtatactaa
    59221 cagttatatc tagttatgga gtagctgttc cagtagtaaa ctctttacct cttaattcat
    59281 taggatttaa ctgggtggtg cctgttataa tagctggaat aattggcaat tttataccat
    59341 caaagagtca atctaataca cttggaacaa actaagtttt atttaattaa aatataatca
    59401 attgtaaata aatgagttaa ctaaaagtaa aaggagttat ttgtacaaat aactcctttt
    59461 atttt
//
LOCUS       DBGJFU010000008        59372 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.008, whole genome shotgun sequence.
ACCESSION   DBGJFU010000008 DBGJFU010000000
VERSION     DBGJFU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.008"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            136..765
                     /locus_tag="ACOIAS_000491"
     CDS             136..765
                     /locus_tag="ACOIAS_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HGZ2700222.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRSVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(879..2393)
                     /gene="glpK"
                     /locus_tag="ACOIAS_000492"
     CDS             complement(879..2393)
                     /gene="glpK"
                     /locus_tag="ACOIAS_000492"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGZ2700223.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2637..3179
                     /locus_tag="ACOIAS_000493"
     CDS             2637..3179
                     /locus_tag="ACOIAS_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2700224.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3184..5685
                     /locus_tag="ACOIAS_000494"
     CDS             3184..5685
                     /locus_tag="ACOIAS_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2700225.1"
                     /translation="MFTLNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPAR
                     TIIQNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLI
                     CGLSSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIA
                     NILGSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGT
                     LMLICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILS
                     FEYFLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIA
                     APLSGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFT
                     MGTPLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDL
                     VGKPSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKS
                     LLNKVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGE
                     NKLNSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKE
                     LTTAYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVN
                     EQKESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQ
                     KKDMEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTL
                     QSTLNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6106..6504
                     /locus_tag="ACOIAS_000495"
     CDS             6106..6504
                     /locus_tag="ACOIAS_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700226.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(6721..6930)
                     /locus_tag="ACOIAS_000496"
     CDS             complement(6721..6930)
                     /locus_tag="ACOIAS_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700227.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(6962..7171)
                     /locus_tag="ACOIAS_000497"
     CDS             complement(6962..7171)
                     /locus_tag="ACOIAS_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGZ2700228.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7470..7889
                     /locus_tag="ACOIAS_000498"
     CDS             7470..7889
                     /locus_tag="ACOIAS_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700229.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            8117..8572
                     /locus_tag="ACOIAS_000499"
     CDS             8117..8572
                     /locus_tag="ACOIAS_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700230.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(8779..9039)
                     /locus_tag="ACOIAS_000500"
     CDS             complement(8779..9039)
                     /locus_tag="ACOIAS_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700231.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(9302..9655)
                     /locus_tag="ACOIAS_000501"
     CDS             complement(9302..9655)
                     /locus_tag="ACOIAS_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HGZ2700232.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANVEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(9672..14993)
                     /locus_tag="ACOIAS_000502"
     CDS             complement(9672..14993)
                     /locus_tag="ACOIAS_000502"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700233.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(15010..15993)
                     /locus_tag="ACOIAS_000503"
     CDS             complement(15010..15993)
                     /locus_tag="ACOIAS_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGZ2700234.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(15994..16692)
                     /locus_tag="ACOIAS_000504"
     CDS             complement(15994..16692)
                     /locus_tag="ACOIAS_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HGZ2700235.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(16689..17741)
                     /locus_tag="ACOIAS_000505"
     CDS             complement(16689..17741)
                     /locus_tag="ACOIAS_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGZ2700236.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(17734..18162)
                     /locus_tag="ACOIAS_000506"
     CDS             complement(17734..18162)
                     /locus_tag="ACOIAS_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HGZ2700237.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(18162..18488)
                     /locus_tag="ACOIAS_000507"
     CDS             complement(18162..18488)
                     /locus_tag="ACOIAS_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HGZ2700238.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(18504..20033)
                     /locus_tag="ACOIAS_000508"
     CDS             complement(18504..20033)
                     /locus_tag="ACOIAS_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGZ2700239.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(20315..20737)
                     /locus_tag="ACOIAS_000509"
     CDS             complement(20315..20737)
                     /locus_tag="ACOIAS_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HGZ2700240.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(20737..23190)
                     /locus_tag="ACOIAS_000510"
     CDS             complement(20737..23190)
                     /locus_tag="ACOIAS_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700241.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(23190..23357)
                     /locus_tag="ACOIAS_000511"
     CDS             complement(23190..23357)
                     /locus_tag="ACOIAS_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700242.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(23384..23830)
                     /locus_tag="ACOIAS_000512"
     CDS             complement(23384..23830)
                     /locus_tag="ACOIAS_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGZ2700243.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(23944..24372)
                     /locus_tag="ACOIAS_000513"
     CDS             complement(23944..24372)
                     /locus_tag="ACOIAS_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGZ2700244.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(24386..25450)
                     /locus_tag="ACOIAS_000514"
     CDS             complement(24386..25450)
                     /locus_tag="ACOIAS_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGZ2700245.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(25455..25898)
                     /locus_tag="ACOIAS_000515"
     CDS             complement(25455..25898)
                     /locus_tag="ACOIAS_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HGZ2700246.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(26147..26587)
                     /locus_tag="ACOIAS_000516"
     CDS             complement(26147..26587)
                     /locus_tag="ACOIAS_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700247.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(26692..26889)
                     /locus_tag="ACOIAS_000517"
     CDS             complement(26692..26889)
                     /locus_tag="ACOIAS_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700248.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            27058..27378
                     /locus_tag="ACOIAS_000518"
     CDS             27058..27378
                     /locus_tag="ACOIAS_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700249.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            27460..27966
                     /locus_tag="ACOIAS_000519"
     CDS             27460..27966
                     /locus_tag="ACOIAS_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGZ2700250.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            28667..29422
                     /locus_tag="ACOIAS_000520"
     CDS             28667..29422
                     /locus_tag="ACOIAS_000520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2700251.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(29565..29993)
                     /locus_tag="ACOIAS_000521"
     CDS             complement(29565..29993)
                     /locus_tag="ACOIAS_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGZ2700252.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(30414..30614)
                     /locus_tag="ACOIAS_000522"
     CDS             complement(30414..30614)
                     /locus_tag="ACOIAS_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGZ2700253.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(30797..31951)
                     /locus_tag="ACOIAS_000523"
     CDS             complement(30797..31951)
                     /locus_tag="ACOIAS_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HGZ2700254.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            32202..33023
                     /locus_tag="ACOIAS_000524"
     CDS             32202..33023
                     /locus_tag="ACOIAS_000524"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HGZ2700255.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRHEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(33099..34487)
                     /locus_tag="ACOIAS_000525"
     CDS             complement(33099..34487)
                     /locus_tag="ACOIAS_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HGZ2700256.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(34622..35380)
                     /locus_tag="ACOIAS_000526"
     CDS             complement(34622..35380)
                     /locus_tag="ACOIAS_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HGZ2700257.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFASSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(35382..36098)
                     /locus_tag="ACOIAS_000527"
     CDS             complement(35382..36098)
                     /locus_tag="ACOIAS_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HGZ2700258.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(36100..36807)
                     /locus_tag="ACOIAS_000528"
     CDS             complement(36100..36807)
                     /locus_tag="ACOIAS_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HGZ2700259.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGQ"
     gene            complement(36923..37459)
                     /locus_tag="ACOIAS_000529"
     CDS             complement(36923..37459)
                     /locus_tag="ACOIAS_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2700260.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(37561..38631)
                     /locus_tag="ACOIAS_000530"
     CDS             complement(37561..38631)
                     /locus_tag="ACOIAS_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HGZ2700261.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(38624..39682)
                     /locus_tag="ACOIAS_000531"
     CDS             complement(38624..39682)
                     /locus_tag="ACOIAS_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HGZ2700262.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(39669..40001)
                     /locus_tag="ACOIAS_000532"
     CDS             complement(39669..40001)
                     /locus_tag="ACOIAS_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGZ2700263.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            40200..41321
                     /locus_tag="ACOIAS_000533"
     CDS             40200..41321
                     /locus_tag="ACOIAS_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2700264.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKITDYISELIDNHYS"
     gene            complement(41449..43347)
                     /locus_tag="ACOIAS_000534"
     CDS             complement(41449..43347)
                     /locus_tag="ACOIAS_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HGZ2700265.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            43662..44438
                     /locus_tag="ACOIAS_000535"
     CDS             43662..44438
                     /locus_tag="ACOIAS_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HGZ2700266.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(44530..45282)
                     /locus_tag="ACOIAS_000536"
     CDS             complement(44530..45282)
                     /locus_tag="ACOIAS_000536"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGZ2700267.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(45443..46393)
                     /gene="galT"
                     /locus_tag="ACOIAS_000537"
     CDS             complement(45443..46393)
                     /gene="galT"
                     /locus_tag="ACOIAS_000537"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGZ2700268.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            46778..47974
                     /locus_tag="ACOIAS_000538"
     CDS             46778..47974
                     /locus_tag="ACOIAS_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGZ2700269.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(48086..49432)
                     /locus_tag="ACOIAS_000539"
     CDS             complement(48086..49432)
                     /locus_tag="ACOIAS_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGZ2700270.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAISNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(49565..50317)
                     /locus_tag="ACOIAS_000540"
     CDS             complement(49565..50317)
                     /locus_tag="ACOIAS_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2700271.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(50465..52042)
                     /locus_tag="ACOIAS_000541"
     CDS             complement(50465..52042)
                     /locus_tag="ACOIAS_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2700272.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGIIAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(52454..52906)
                     /locus_tag="ACOIAS_000542"
     CDS             complement(52454..52906)
                     /locus_tag="ACOIAS_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2700273.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(53217..54662)
                     /gene="purB"
                     /locus_tag="ACOIAS_000543"
     CDS             complement(53217..54662)
                     /gene="purB"
                     /locus_tag="ACOIAS_000543"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGZ2700274.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(54806..55714)
                     /locus_tag="ACOIAS_000544"
     CDS             complement(54806..55714)
                     /locus_tag="ACOIAS_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2700275.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            55971..56795
                     /locus_tag="ACOIAS_000545"
     CDS             55971..56795
                     /locus_tag="ACOIAS_000545"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGZ2700276.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            56835..57365
                     /gene="lepB"
                     /locus_tag="ACOIAS_000546"
     CDS             56835..57365
                     /gene="lepB"
                     /locus_tag="ACOIAS_000546"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGZ2700277.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(57447..59228)
                     /locus_tag="ACOIAS_000547"
     CDS             complement(57447..59228)
                     /locus_tag="ACOIAS_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGZ2700278.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT        19796 a         9656 c         6721 g        23199 t
ORIGIN      
        1 atgcatgtta aaatattatt ttaaaacatg cacaatattt gtacatattg tagacatata
       61 aacctataaa ttatacaata gtattgttaa gattatatat ttttttaaca taaattttgt
      121 attgggggaa aaacaatgga taaaaactat acgatgccaa tataccaaaa aatagcgttg
      181 gatattgcaa ataaaatata cacgggtgaa atacaagagg attcagtact atttggacgc
      241 tctgttttgg ctggtaaata caatgtatct ccagaaacga taagaagatc tgtcaaaatt
      301 ttagaggaca ttggcgttgt taaaagcata aagggtaaag gtgttattgt actatctcca
      361 gacaaagctt cttcttttat aaaaaaatat agagatatta caaatatatc atcatataaa
      421 tcaacacttt ataatttaat agatactaaa tctaatttag aaaatgaaat attagatact
      481 ataaataaaa ttttagacta ttctaacaga cttgaaataa tcaatccttt agtgcccgtt
      541 cagttcacca taaattctaa ttgcaaatat attggtcaaa cagctgcaca aactaaattt
      601 tggcaaaata caggtgctac aatagttgca attaaaagag gtgaagaact tataatatct
      661 cctggaccat atattgaatt tttagaagga gacattctct tagtagttgg tgaccaacac
      721 atttacaatt ctataccaat gtttttatat gaaaacgaaa aataaattat ataatttagt
      781 atgattatat aaaaatgcaa aattaacaac aaaaaaagag cttcatattt tacataaagc
      841 tctttttaaa ttcaaataaa tttaactttg attaattttt aaatatccaa ttcatcttct
      901 ttttcccagc ttaaagctct cgatacagct tttttccatc ctttatataa tttacatctc
      961 ttctcttcac tcatatttgg attaaactct ttttctactg accactttga agtaatttca
     1021 tttcttccaa tatagaatcc aacagacaac cctgctaaat aagcagctcc taaagctgta
     1081 gtttctacta ttttgggtct atctatatct acatttaata tatctgattg aaattgcatt
     1141 aaaaaattat tattacttgc tccaccatca acttttaatg attttagttt taattttgaa
     1201 tcgttttgca ttgcttctaa aacatccttt gtctgataag caattgattc caatgtggct
     1261 cttactatat gttctttttt tgctcctctt gtaagtccta aaattgaacc cctagcatac
     1321 atatcccaat atggagctcc aagacctgta aatgctggca ctacatatac accattagta
     1381 tcctctaccc tatttgcata catttcacta tcttttgcag attcaattat ctttaattca
     1441 tctctaagcc attgaacaga tgctcctcca ataaatatac ttccttctaa tgcatatgtt
     1501 acttttccat caactcccca agcaattgtt gtaagtaacc catgcttaga tttaactatg
     1561 ttactgcctg tattcataag taagaaacaa cccgttccat aagtattttt tgcagtacct
     1621 tcttcaacac aattttgacc aaataatgct gcttgttggt ctccagcaca tcccgcaata
     1681 ggtattttag ctcctgataa catgcgctca tctgtatgac cataaacata gctagaaggt
     1741 tttactaatg gcaacatgct tattggtatg tccaattctc gtaatatatc ctcatcccat
     1801 tctaaatcat ttatattgta catcattgtt ctagatgcat ttgagtaatc tgttacatgt
     1861 acttttccac gagttaaatt ccatattaac catgtatcta tagttccaaa taataactct
     1921 ccttgttctg ctttttccct agctccttct acattatcta atatccattt tacttttgtt
     1981 gctgaaaagt atgcatctat taaaagacct gttttttctt ttattgattc ttccaatcct
     2041 ttatttttaa gttcatcaca tatttctgac gttctcctac actgccatac tattgcgtta
     2101 taaataggtt taccagtata cctactccaa acaaccgtag tctctctttg attagttatc
     2161 cctattgaag caatttcttc tggtcttata cctgctgttt ctataacttc tctcattacc
     2221 ccactttgtg acccccatat ttccataggg tcatgctcta cccatccagc ttttggataa
     2281 atttgattga actctttttg tgcaattttc actatttcgc cttttttatt aaatagtata
     2341 gctcttgaac tggttgttcc ttggtctaat gccattacat acttcttttc catcaccata
     2401 ttctccttat catataatat ttaatttatt aaaaagaaaa atttaatatc acatatttca
     2461 ataaataatc atacttaagt acaaatttaa tataatagat atttagtaag aagacactta
     2521 ttttctatca tatctatata ataatattat acattatagc ttacatattg attaataaat
     2581 tttaaaggag tataataaac agtgttcata ataattttaa taaaagaggt ggtaatatgc
     2641 caaagatttt agaaaatgta aaagaagata tattaaaagt gtctcgggat atgattttag
     2701 aagaagatta ctcgaatatt agcataagga aaatagctca aaggtgtggt ataagtgcag
     2761 gtacagtgta taattacttt aattcaaaac aagaaataat agaatatatc acaaaatctg
     2821 aatgggattt attgataagg cgaattgaat actccaataa aaacactgaa gattacataa
     2881 aaaaactaaa cattattttt actgaaataa gaaattttat aaacaaagtt cataatatcc
     2941 aatacaatga ctttttaaac acttttgaaa cagagagatt ttttgaaatg aaaaaacaca
     3001 aagacgactt tcatgaacag ctttcaaata aagtatatga agctttagaa aaagaaagtt
     3061 tttttaataa tgacaaatta gtatgcaata tcataataaa aatgtttttt tcatattcaa
     3121 catatcttca tatagaattt gaagatttaa aaccctatat tgaaaaatta atacattaag
     3181 gtggtgttta ccttgaatca taatactagt attaagaaca aggacagtaa tccaaaaata
     3241 tttttagtac ttacacttta tctattaggt atatttatgg gagctattga tactggaata
     3301 gtatcaccag caagaacgat tatacaaaat agccttggag taaatgaaaa aacgggaatt
     3361 tggatgatta ccatatacac attaacatac gcttctgtaa ttcctatctc tggaaaatta
     3421 gcagataaaa taggaagaaa atatgtgtat ctagttagta tatttctatt tggtagtggt
     3481 tcattaatat gtggattatc atcactattt tccaatttct atattttatt aattggtcgt
     3541 gttattcaag caattggtgg tggtggaatc atgccaattg ctacagcaga atttggaact
     3601 accttcccag aaaacaaaag aggcatggct ttaggtttag ttggggcaac atatggtatt
     3661 gccaatattt taggttcaag cattggtagc acaattctaa gtatatttgg aactcaaaat
     3721 tggaagtggc tattttttgt aaatcttcca atttgtttaa tcatacttat tggtggagtt
     3781 ttctgtatta aaaacaataa gtctgaatct aaagaaaaaa tagataagct tggtacattg
     3841 atgttgatat gtataatcgt atcactatta tatggtctta tgaatataga cttttttaat
     3901 tttaaaaatt ctatacaaga tatatcagtt tatccatatt tattgacatt tattgtactt
     3961 atacctattt tcatatttat tgaaaataga gctaaagacc ctattttaag ctttgaatat
     4021 tttttaaatc cgagaatatt gatagtactt attttatctt taattgttgg tattggaatg
     4081 atgggtatgg tattcgttcc acagtatgct gaaaatgcat taaagataaa tgctggttct
     4141 ggtggatatt ttgtagctat attgggatta tttgcaggta ttgctgctcc tttaagcgga
     4201 agacttattg acaagtttgg ggcaaaaaaa atactattac taggattttc tgtatctatg
     4261 ataggctctc tttacctagt tttaattgct ctaaaaacca atactgtatt tagtgtatgt
     4321 gtaagtttaa tatttatggg attaggaatg ggatttacta tgggaactcc tctaaactat
     4381 atgatgttat caaatacaag attagaagaa tctaattcag cacttgcaac attatccttg
     4441 atacgctcaa ttggtacttc aatttctcca gctataatga ttggatttat agcacatgca
     4501 ggattatctg tgcaagataa tattatggac ttagttggaa agccctcaac tcctaaaata
     4561 gttcaacttg aagaacttga aaacatgata gatgacttaa aatcagaccc tgaaatggca
     4621 aaaaatttaa aaaatgtctc tatcccaaat atgaatgata cttcaaatat aaatatggac
     4681 atgacaggtg gcaaacttcc aaaatcctta cttaataaag ttcagtcagc agatacaacc
     4741 aacattacca atataacaaa agaaatatca acaacaatgt ttgatgaaaa agttccatct
     4801 ataattacta aaatacagaa aaatgtacaa aaaggaatag atggaacaca aagtggaata
     4861 aacggtattg aagatggtga aaataaacta aatagtggta ttaaaggagt tcaaactggt
     4921 attgacaata tggtaaaagc tagagctggt attaatcaag gaatagatgg tgtaaaaaaa
     4981 ggtattgctg gtgttgacaa aggtataaaa ggtatggaac aaggtcttga aaaacaagat
     5041 gaggccatca aggaacttac aactgcttat aataaaatac ctactacaga aaaaactcct
     5101 actgataacg aaaataactc aaacgaacaa aaccagagta ataccagtaa ttctgaatca
     5161 gatgacacta agaatgattc tacagaaaat aagaataaca attccagcga gaataaacca
     5221 aatgtaaatg aacaaaaaga aagtttaaat gctcaaatac agcaactaaa aaagacaaga
     5281 gatgacttaa atacaaagtt acaaaaaaat aaaactcaaa agaacgaatt aagtaaaaaa
     5341 ctaagatcaa tggaatctca aaagaaagag ctacaaagca agttaaataa ttctataagc
     5401 caaaagaaag acatggaaaa aagccttgat acaatgcaac agcaaaaaaa atcactccaa
     5461 gcagttttag caaagacaca agaggtaaaa actgaaatac caaaggcttt tgataaatca
     5521 aaattggatt atataaattc catagaaaat aatagaacaa aaatagaaaa cacactacaa
     5581 tctactctaa actcaggatt taaacagatg tatataacag tattctgtgt aaatctacta
     5641 gcatttataa tattattgtt ctataaagaa aataaaacta gataaaagag tgttatagta
     5701 ttatattgta aatttatctt attcttaaat attttattct aaagaataaa ttcataaaat
     5761 ttttgtaatt gattaatata aattacaata taatactatt tttctcttaa tctttttctc
     5821 ttttacagac aatcaaaaag caatcttagt ttatcgaaat aaatttatac aatctattca
     5881 tacacccctt tacatttata taacaattaa acctattgtt atatatacaa atcaaaataa
     5941 accatatatt ttcaactata atatgttaaa atcatattat gagcatatta atttaagcaa
     6001 aatgtcattt agactttaat ttagtagttt tatattataa atttatatat ttttaatttt
     6061 aaatgtcatt tagacattac tcataaaaag aaaggagtgt attttgtgga tactttagga
     6121 aaaagaattg cttatttgag aaactccaaa aaacttactc agcgcaaact tatggatatt
     6181 ttaaaatttg aaaacttagg caagtatgaa acaggagata gaaaacctaa ttgtgatata
     6241 ctaatgtcaa tagctgatta ttttaatgtt acgactgact ggcttctcta tggtaaagag
     6301 aaggtaaatg tcaatagtag tgtaaaagaa gataaagaag attacttaca tgttactaat
     6361 gatgaaatga cgatattaaa tctatacaga caactaaacg aacgtgataa aataaaaata
     6421 gaaggaatac ttgaacttaa gatatctgaa tataaagact taaaaaagca ttcctctaat
     6481 aataatgaag ataaaatggt ttaaacatac ataatttgat acgattttac atcatctatt
     6541 aaccttcttt ttttgtagta tattactaaa atatggatat tggattattt aaaaacaagt
     6601 atagtctaat ttcaagcaca tttataatat ttagattatc ttatccatat tttagtttaa
     6661 gtactttgaa aaatataata catctctatg tataacttat attcctaaga aatgcattac
     6721 tcatgttttt aattcttcca acatctttct atacacaatt gtacatcctt cataattccc
     6781 ctctttaaga aggattgaac atgtactaga atatttagtt aaagtattta atctttccat
     6841 atttaagctt tttatattac tgagtgctgt aataaaatca tcaattgctc tacaacactc
     6901 tataacccca tctgaatcca atttacacac aagaatattc acctacttta tatactattt
     6961 attaatcatc ttctgaatat tctatattaa gtaattgata aattgatgat ttatattttt
     7021 tacctgttct tcttccattt aaaatagctg acaaatagtt ttcattaaca cctattttct
     7081 tcgctaattc cctttgagtc atatttaatt caataagtct ttgcttgact tgcactccca
     7141 aaaaaggtat ccattttttt cgagcatcca tttatcaact tcctttctaa gcgatttata
     7201 tttactttta tattatttta gcttacgcac cccctctatt tagtatatat ttttaatatg
     7261 tacacatatt aagtatataa atttatttaa tactaaataa gattttttga ttactttgtg
     7321 ctaaaattag gatgtaaatt cgtatcatta agttaattat aaagtcattt tgaccttaag
     7381 tcaatggttt ttatgtcatt ttgaccttta atttgaaact atttacatct taaaaagtct
     7441 ttttgacatt aattgaatgg agagtgttta tggatacttt aggagaaaga attgtttatt
     7501 tgagaaaagc aaaaaacctt aaacaatatg aactagaaga aatgttgggc tgtgacaact
     7561 taagtaaatt tgagagaaac attagaaaac caaattatga aatattaaaa tctatagctg
     7621 agatatttaa tgtttcagta gactggctat taaatggaga taatctatca cataaatcag
     7681 atttaatttg cgattcctcc tcaaactatc cacttaatag cataaactct aatgaaataa
     7741 agcttttaaa caattttaga aaattaaatg attatgataa agctaaaata gaaggtatga
     7801 ttgaattaaa acttcatgaa tatgaaaaag agaaggattt aggaaaaatt gagtataaca
     7861 aaaataaaga tgagaaaata gataaataat ttttatgttg cttttgatac gattttacat
     7921 catttctttg ttctaatatt tttatttcat ttattttctt ttttataatt ttatgataaa
     7981 ataattagta tattttgctt tacttgaatg taattattta tacaaaagtc aaacataata
     8041 taaatgtagt aaatttttag tttcaagtaa tatctcgtta ttataactaa atatttatta
     8101 aaattaggag gataaaatgg gcttaagaga caaattcgcg caatcatttg ctagatcaaa
     8161 aactatgagt ggccctgaga aaaaggctaa tgaaataatg ggtaaattgc ttcttaagaa
     8221 agctatatta cctatcgttt taatgttcgt gataataatc gctggagcta tgcttaaaat
     8281 caattcttgg gttacacttg gcataaattt agttattgct gttggtgctt tcttctacat
     8341 aagaaattct agtaaaaagt atcagaactt taaaccttat gttggcaatt tgattagttt
     8401 agagaaaaaa ggtaaaaaag agtacgttgc tattataaaa caaggtaagt taccagttaa
     8461 gttacaaata gcttatggtg gtgaagacct tgaacatgtt aagaaaaatc aaatggttca
     8521 aataagttat aatccagatg ctaaaatagc tattttggtt aatagacaat aatttaataa
     8581 aaaaaaggag ttgtcttaaa attgattttt tctaatcaaa tttaagctca actccttttt
     8641 taatttttat atgttatttt tattactttt tttaattaat attttaaata ttaaatcctt
     8701 ctcaataaaa tagtgaacta tctacaatac tactttatct tttcctaaca atatataatc
     8761 tggtgctact ctttcatgtt atacaccatt ttgtaacata ccttttatat cagtaacatc
     8821 ttccttaatt tcctctacat ctgttttcat tatacccatt tgaacaagta tatttttgtt
     8881 tatttcttgt tgttgtgtag ataattctat aaaattttct acagtttttt tatacatgtc
     8941 tctatcttct tttttctcct gcatagtatt tttaaataat agagcacata gtatacctat
     9001 tgcccctaga ctactcaatt cggttattaa tttatccatt atagcctcct attgtctaaa
     9061 ttttttatac ttatagcttt aatttttgtt gtattcttac atcatgcaat attatatttt
     9121 caggattatc aatttaatct tctgtacttt aaattgtagg tgtaatttat agattgaata
     9181 aataataaat atatatacaa gttctaatgt aacaaaaaat aagtacaagg cttacttatt
     9241 aaagttaaga ccttgtactt tacataaaaa aaataactaa tacatagtta ttctaaattt
     9301 actattcaac tggtatttct aattctttac atctaattct aacttgctct ctaattactg
     9361 ctggtacttc ttctattgtt tttttgcctc ttaaaattaa agctacatat acttctacca
     9421 ttccactctc acctccagat tttcttacac taaaagtgct tatattactt gcttcattga
     9481 cagaactttg atttaacaac atctcatata tttctacatt tgctataaaa ttatttgtta
     9541 tatcatcatc ttgctttact tgacctatac ttatgtctga aatgtcttta tactgcattt
     9601 ctacttttct accttgtttg atttgttcta ttttattttt atcgaattta aacaattttc
     9661 acacctccat tttaagcttg tggctcgttg ttttcagcat catatctaaa aatcatagat
     9721 ggacgtttta tagctgtcca tttatctcct ttttctacta tgattttagg ctgagcactt
     9781 tttatgttgg cttcttctaa ttcatagatt tcttggtaac ttgagtctga ttttttgatt
     9841 tcaactagtt tcatgtcttt gaataagtca gatttattta tttttacttg aactttagtt
     9901 ttatttatta attgatatat aggattgttt tccatcatat catcctttaa tccctcattt
     9961 aatgttacta ttaagtcagt gttattttca gtaatgttta tgattgaata tactttattg
    10021 tttatcatta aaatagaatc cttttttact ttaagccctg tttctgattt aaatataaac
    10081 atacttgaat ataaatcgcc tgtaggacta ctggctttaa tcagtatctt attttctcct
    10141 aatactattt tattttgtgg tatctcaaat ataatgctat ttatttccaa attagtctta
    10201 gtagatatta attcatcatt tataaacatt tcaagttttt ctataacatt attagggtct
    10261 tgtactacaa ctctcacttg agatattgat ttttcaacta tttctataga tgctttactt
    10321 gtatctactt ttgttatcat gtatattgta tcatctgaaa gctctatatc taaaatatca
    10381 tttagatgtt caggaattgg tattttagta aattcatata aatcagtaat tgatatagat
    10441 attttataca tagatttatt tttaccagtt atccatagac tattatcatt tttaattact
    10501 agtatgtggt catcattatg tgacttaatt tctttaacat tagttaatac tttagtaaaa
    10561 gttcttatta tcttatcttt ttcactttct cctagtccaa ataatccatt tgtattgtac
    10621 ccttgagcgt acaactctcc ctcattagtt aaaacaagtg tcattgagtt atatatactt
    10681 atatctttaa cagaaggtaa tccagtaacc ttttgtaact ctcttacatc attattgtgg
    10741 ccaactccca attggtactc agtattcttt ccatatgacc atagagtatt atctgtctta
    10801 ataatatagg agtagttatc tccacaagca acttttttga catctgatat atttacttca
    10861 gtaagagtta ataattctat atcttcaagg gtttgattaa tttctttata tgaattactt
    10921 cctattccat ataacttgcc atccttagca acagccaatg aatgtgtttt tgatatatca
    10981 atatcagata cctcaaatgg caatattatt gaagtatact taaaattatc tttaaatatg
    11041 tttttataat ttatatactt attatttgtt tttatatact tatcattaaa cattactagc
    11101 ttttcatttt tcttattaaa aactatttta tctgatgtgt tatctgtttt aaaatatgaa
    11161 tgtagggaac cttcaagagc ttcattttta tcattatcat atctatggtg aatacctaaa
    11221 tgattatatc tattatctcc tgttgaagaa taaaattttc catatatatc tttaataaat
    11281 atagtattgt aactagaaaa tacagtatca acatctttct ttatagatac tttataatat
    11341 ggaactgaca tatccctata agattcatca gatataccat aagtatctct acctgatgat
    11401 atcaaattgt tattattatc tatataaagt gtattattag ctgttacaaa aacatcttta
    11461 acatcttcca cactatttgt attattatta tttgctatac ttccatatcc actaagacct
    11521 ccaacaaata gtttattgtc atttgttaaa atggctgaag tatatccatt aactactatt
    11581 ttttttgcat ttataatatt attaattact cgtatcccct ttatatcatt agtctctgtt
    11641 gaaaagaata ccctctcttt tttccctgta gtataaatca tattgtcatt tgttataaaa
    11701 aatgtagctt cacttcctgc tgtcacaaca gattttacat tctcaatatt tatttttgta
    11761 aatgtactta tacttccttc agtaaaccca aaatagccat ttacaagtcc acatacatag
    11821 catgtattat catttttaac aatgattgat acatcattac atagatatac atctctaaca
    11881 ccatttatag gtatacgtgt aaatatattc ctacttgtag tatcacccaa tcctaactgt
    11941 ccttttccat tattacctgc tccatataaa agaccatcat tagttaataa tagagatgtg
    12001 tttccatata cagctacata ttttacattt tcaacattta ttttagtaaa tatatttcta
    12061 tttacattat ctcctattcc taactgacca gaattattta aaccaacagc ataacaggta
    12121 ttatcatttt ttatcgcaaa tgcatgttta ctattgtcag taaatctatt tacatttata
    12181 gattttatat tatcaatatt tattttagta aatacctttc tatttatctc atctcccact
    12241 cctaattgac ctacatcatt taaaccaaca ctgtaaagca tattattatt agttaataaa
    12301 tatgtagact ttgaatggct atatatttct tgtatattag aagatatttc actactgcta
    12361 ttctttttat tgtaatttat cttattattt aaatttaaac tactattaac tgatatacca
    12421 tgcgtaccct tagagttata tccacatata aataagctct tatcagttaa tattgcaaat
    12481 ctagttttat ctctacttaa atcataagga ggtataaata tattttttat atttttcatt
    12541 acctttttat aattagtggc attatatcca tcttgtgtat ctgaaacatc tagttctttt
    12601 gcactttcag atatattccc aaaaccatat agcgtgttag aagataaata caatagagct
    12661 ttatataaaa atatggcttt atcagaggta ataggacctg aatttatatt agatgctgca
    12721 atattattag atgattgatt gttaagataa ttccttccat attgatgtat ataagatgaa
    12781 cctccagaaa taagtaaatt atctggattg acatttttta aattacttga cgattcccca
    12841 ttatataaat aagcaaaagt tgtagaagat gatgattgtg tacatcttgt aggataataa
    12901 ttatatatat aagaatcaag atatcctgca tatgaaattc cctgatattg aactctatag
    12961 cttgaatcag ttggttttgt ccatgaagta ccatggtatc cagttagaaa agatccaatg
    13021 tttgtacaaa atacagttac atactcatat atgataatat tattaatttt agtaatatta
    13081 ttcgtgcatg aaactctact ataacttgac cctgatatat ttatataata ccaatcactt
    13141 aataaaccta gatttacatt agatccatca gaagtactaa aacctaacag gtaatttgta
    13201 tttataccag ttgcatatac ctttttttca tcaactacaa taaatgttgc attatatgaa
    13261 ctataagcat cagttacatt actaaggcta atctcagtat attgacttat taatgcatta
    13321 ctaagtccat taacataact tgaactcttt ccagaagtgt atatttgctt attattagtt
    13381 acaattaatg aaaaccagtt tgataaatca gtatttataa caactttttt tacattatct
    13441 atagatattt tcttaaatac atctgaatta gaaggaagaa cacctgctat tgagttacta
    13501 tttggtccag aataataaca agtatcatca tttttaatgt agaatattcc tccagaaata
    13561 ttttttgatg ctactataga ctttacatta gagatactta attttgtcaa ctcatttaat
    13621 tgacttggta agtcagaacc cattcctttg tatctattat ttcctagacc atatacatct
    13681 ccattcccta aaataaccag agtacaataa ggactaaaaa cgacatctct tacagaatgt
    13741 atatctagtg gaagttcaat atcctttatt tccatttctt cttttaataa agttaattta
    13801 tcctcagatt taattaaatt tatataatta ctataactat atttttcact atccattttc
    13861 ttaaattcta caatgtgcat atcatcaaaa aacgaaccta attgtccata agtattatct
    13921 cctgtacaat atgaatcatt atttgaatca attagtatgc tatgtgataa accaatgaaa
    13981 atattcttag ctacaatatt ttttaaatct ataaatgaat tatctatatt ctcagagtaa
    14041 tttgctatac ctaactgata tatatcatta tttccagcaa cctttaaagt acctttgtta
    14101 tctataagca tagtgttacc atgaccacat gcaatagttt ttatatttac ttcttctatc
    14161 ttagtaaata gtgatgattg aagtgcattt ccaaaacatt tttcctttcc acaaccatat
    14221 atatcattat tatacgtcat taacacagtg tgttcagaac cacaagctat agtcttaaca
    14281 tcattaatag aaactaaact aaataaatct ctattattct tatctcctaa acctagttga
    14341 ccttgattat taaaaccagt agaatagcat ttattgtcat tctttattat aaaagctgat
    14401 ttattatatg aaaatacagc ctttacattg tcaacatcta cttttgtaaa tgtatttaca
    14461 tttgcagatg ctccattagt tcctagacct aattgaccat acaaattatt tccagcaaca
    14521 tataaagttc catcattgta taaaagatat gttgattgaa gtccacatgc aatatccttt
    14581 acgcctttta tttctggaac cttctcaaat gtagaaactg ttttccctac tttttctaat
    14641 cccaatccca tttgaccatc tgtatttata cctgcgacaa gtaattctcc atctgaagta
    14701 agtataactg catgagattc tccacatgca acttttttaa tattggtaga tatatttggt
    14761 attttcgtaa ataattttct tgttgtattg tcttctccca atccaagttg aaagttacta
    14821 ttatctccac atccatacaa gatattatta ttttttagac caaatgtata gttacaacat
    14881 ccagtaaaaa aattaataaa gtcataatta tcataccctt catcaaattc atcaactacc
    14941 atatttttat cgccaaagaa attacctctt tgaagtagtt tagttctttt cattataaaa
    15001 acctcctaat tatcttattt catacgaata tttatttaaa gttatatctg tctgaccaat
    15061 tgaatatgcc ctattagatt ttgtttcaac aaagttatta tccatttcat gtatatatct
    15121 aactctattt gcattaaatg gtagcaccac attcttaaat gtaacactac caagttctcc
    15181 agttatacac tttctattta aatctaactt aaggccttct atagctatta tgtttttagc
    15241 attagttaag gtatcatacc aatatccagc ttcatctaca tctatttctg actctaattc
    15301 taagtatatt aagatatcta atactttttc agataattta ttgtaactat tttttaatac
    15361 agatagttca gtttgtattt gacctcctat tacatcatca atagatatat ttttaatttt
    15421 ttctactacc tcatttatag ctcctgataa gtttttagct tctgtagata agagttcttc
    15481 gccaattttt acttttattt cttttatatt tttatcaaac ttatcatcta aatcttgaat
    15541 atccttttga gttacaaaaa ccaacgtcgg gtctacttct aatgtaacat ttgaagtatt
    15601 ggacaattgt aaaatcattt ttacaaccaa atctttaata ctgccatctt cagcacgtgg
    15661 cttatacgtc tctggatact tacctatagc tattatatta ttctcattat ccagaacagc
    15721 agcttctctt atcataaatc caccaactgt tcctggtaaa atagtctcta taactatcca
    15781 attaggattt ttttcatcaa ttcttagaga atttatattt ccttcccaaa ctacattctt
    15841 taaagcagtt tgttcctctg ttggctcata atattctcca ttactatctc caacttgaaa
    15901 cttggttaat ccaacaagct ctcctagtgc agttgcattt gctatttttt ctctaccaat
    15961 tttggttaat attatataaa atttttcttc caattatacc acctccgatt taggataaac
    16021 tactacattt tctacacctg tatcatttgc aatagctata ttaatcttac aattattttc
    16081 aatgcattta atgcttaaag gataaacctt tatttcttct ctacttatca ttccagctcc
    16141 acagtttaca acagcactac attttataat tggttcaaag tatggatgaa cttttacctc
    16201 ttcactacat accatacctc caccacaata gaccatactt ttatctagta ttattggttc
    16261 taaatagtgt aataagtgag caggttttac tctttcaatc atatcgctac aatctaaaag
    16321 agttttatag tcacagttat ttgctataaa actcaatacg aatgtaaatt catcactata
    16381 aacttttata tctgtttctg attttgtata tgcctcacat atacttttta taacttcaat
    16441 agtactagta cctctgcttc tcattttagc ttttatattg ctacgtcttg tgtcaaaatc
    16501 aagttctttt ttttcaatgc agagtatgtc ttcccacata tctaatcccc atgttgcagt
    16561 gtcaacaaat aattggttaa aggtatcatc aatatcttct ttaagaatat ttaattctgt
    16621 atcgtatgca ccttgtatct cctctacaat gtaatttcta tcaaatgatg gtagtttatc
    16681 aattagcttc attgattttc cacctcacta aaattaacag ttgttacact aggtatttta
    16741 tcttcattaa tagttatatt atctgtactt ccatttataa gtaggttact taaatcgtgt
    16801 acaccagtag tatttataag tattcccatt acttttgtat agattatctc tcctctaata
    16861 tctctaaagt atgtatttat actttctagg aaagattctt ttacattgtc taatgtatat
    16921 ccatcttcta gtttcattac tgcacttata cttatttcta ttggtaatgg tgtcacaact
    16981 gttatagttg gtccaatagg cttctcttca tctatatgtt gctgacacct ttcaattgtt
    17041 tctgtatcaa cagcttgatt atttttccca aatatcaaga ccttaactgt tcccggacca
    17101 tcccatcttg gataaacctt aacattatag actccatcta cttccaaagc ccattcttca
    17161 tagtgagctt tatttccact tgtagcttga tttctttgga ttttataaaa tctttctttt
    17221 agttcttcat ctgtctctat atctgtacca ccttcaaaac ttcttgtgtt agtaatcttt
    17281 gttactccag atatattatc aactagttta aattcacaat ttgtaggtaa attatatttc
    17341 ttaccaactt ccagagcttg aactgggcta ttgtcacctt cttcactacc aatagttaca
    17401 tcttttatta ctacaaatag taaatctcta tatgatatta ttgtgccatt atttattaca
    17461 gtacctttct ctccaataaa ttcaacttct ccatttgact ctgtacctaa ctttctatat
    17521 acaccaaatt cattgactct tttatcaaga aactggttat atgtgtcttg tataaaagcc
    17581 attttatgca tctttgaaag ttctagatat atcttcgaaa gttctagatt atttccagat
    17641 accatgttgt ttagaaaaga accttctcct ttataaatat caagattaat attttcaaga
    17701 gttctatttt ttattacttc atatgtctgg tcactataca tttatttcca cctccccata
    17761 gatagtggat acttttacat ttgcactcaa tatgtctctg ttaaagttag cactttttac
    17821 gtttacttct aatatatatg gatttattag aagggcctct tttatgaatc tactagcttc
    17881 actttctgta agaccttttg tatatttttg tcctattagt tctgaaagct ctgtcccata
    17941 ttctaaagag tatatctcat gttcatatct atttgtcttt atacacttgt atatccaaac
    18001 ttttaaggct tctttttttt caattatttt aaagtcacct ttttctaaaa taggttcatc
    18061 tttttcaaaa tcccatgcca cttcacgaaa gattggcaat tcttctgttt taggtaagat
    18121 ataatcctct gggacaccta taaaaggaaa tattgtactc attataagct caccaactta
    18181 cttacaacag caaatttttc gcctattcta aacattatta ccatatcccc agattcaaag
    18241 ttatctataa aaggattttt tacttcatgc tggtgttctt gatttgtttc tgtgtcaaat
    18301 gtttctagct gtctatcaat catccaacta tctatcaaaa tatcttcttt ttctaatatg
    18361 atgttatttg tctctatttt taaatctggt aatttacttt taatttttcc aataaaaaaa
    18421 gaaggttgat tatgatactt tccttcttct cttattattc ctataaattc atttattgga
    18481 ttagccatta tatcaccatc cttttataat actctcctag ctgtgttaaa atctcttcta
    18541 ctacttaact tacttatttt aactacatca cctttttgtg gagcatgtaa aaattctcca
    18601 ttacctatat ataatccaac atggctgact ggattatgaa aaaatactaa atctcctgct
    18661 tgaagatttt tttgttctac tttcttgcct tttttagatt gttgatttga cgttcttggc
    18721 aaactaacat taactttttt aaaacaatag accattaaac cagaacagtc aaaactactt
    18781 ggtccatttc caccccattt ataaggttta cctagatgtt tttttgcttc tgatattact
    18841 attttagctt tatcagatac attaccagaa taaccaccaa taataatttt tccttttctt
    18901 cttccaaatt tactagcttc ttcaacattt ccaaataaaa tatctatatg gtatgttcca
    18961 ttcttttcta tagttattgc aggtcctacg tcatttactt tatagactcc atcacgacca
    19021 gaaactcctg taattacctg tatttcatct ccatagttta ataaaggatg tttatttaaa
    19081 aactcttttg tataataagt ttgctcatat ttaccaacca taggagcagc gcaagttttt
    19141 ttagatgggt caagtttttt ctttctacaa tctgtatctc caccttcttc ttttctagga
    19201 cagtaagctg taaattctgc tgtaaactct tttcctcctg cataatcttc tccccctaaa
    19261 ttactttctt ccttaggttc atcctgtcct gctgactttt catccataag attttgaaaa
    19321 ttaagttcaa gctcaatttg atattctcca ttttgccaag tatgtttgtc tgtatctata
    19381 taaaatagtc ctacaagctt tgtataagaa tctttaactt ttactcctct accagttata
    19441 caacttacat ctccataacc tttaagagaa cagctttttt ctatcccatt aaactcgcta
    19501 tcaatatcta catcttgatt ttcttgttgc tgaattactt tttgcattat tacatttact
    19561 tccttaaaaa tttcattatc tattttttcg ctaatcttgc ttccatactg gtctaccact
    19621 attactttat tttttacatt ttccatgctc tccgaaaagg tggtatttat aatattaaat
    19681 ccctcttcaa acataacact taatgtaaca gttccttttt caataacatt aaacttatct
    19741 aaattggcct ctatcatata ctttttcttt gtctttttac ttgcttctgt atatgcactc
    19801 attattgtgt cataaccatt tactcctata aacatctttg tatacttgac attggtcttt
    19861 gctattattc caactgaaag cctattttca gcaaatactt gctttgctat gtcttcaact
    19921 aacttatctt taaaattgta tgacacttca ctttgtgtaa gtaaaaatcc catatcttta
    19981 gatacaaaac taatttcatt actgcttgaa tctttagacc tattaattat cattcctcta
    20041 aagagttctt tctcatctac atagaaacag actgtactag ctattggtat atcgatttgt
    20101 tggaaattta catcaaatga tgattgtatt attgaaaact ctagtgtcct tgatggagat
    20161 ttataatcac ctgaccatga taccttgtct actatgtcag ttatgtcata tatacttccg
    20221 ttttttatgt gtacccaaat cttaataata aaaaatcacc tgccttttca tttaattcta
    20281 tcagcagtta tttataactt attatctata atagttattg ttttggagtt acttttggta
    20341 tctttattct tttatactct tttaaatcta atgtaaaata tacatctcga gtcccatctc
    20401 tttcttcata actaaaccct tcaattatga cttccatatt tatatttgtt tccgtaattg
    20461 taaatcttaa tataaaacct tccttcatcc atttttctat cttatttaca caatcatatg
    20521 gtgatggaaa acctgtatag tcacagaaag tatagtcttt atttggaaaa aaacttgata
    20581 ttgaaatgct tttaagagct acaccaccaa aagttgctat ttcacctatt ttgagtatac
    20641 tagaagagtt tacagcagct tttccattaa tactaaagga agatggaaat actggaaatc
    20701 taaatctatc ttcagcttgt ctaagccaca tttccattat aagacacccc ctaactgtgc
    20761 caattggata cttgcaacta attttgatgt tatcttttct atatcagctt cttctctaat
    20821 cactattgta tcagctaact tagctatgtt tatatttcca ccattttttc tgcttctata
    20881 ttgatttgct tcttgtttag ttaaaaccat ttcaccttca tggagtctgg tttgatagtc
    20941 attataagga acatagttca atccaaatgc attattacct ttcttacctg aataagcttt
    21001 accagaatca ccagcctttg ttccagcata tggattatct ttgtaatttt ttttactctt
    21061 ttccactaga tttaaaactg ttcctgtgat gcttccattt tttatcttat taatcatagt
    21121 ttgccaccat ttacatatat ctttaactat tttacatact ttatctaata tgttaaaaaa
    21181 agctcccaat attggctcta taattttcca tccagtttcc aacagaggtc ctaaggtctc
    21241 ccatacagtt ttccaaataa cacccagtgt ctctataaaa ttgtttattt gaggtgcata
    21301 ttcacctaaa aaagtaaaaa ttcttgtgat aataggacta acagcttcaa ctacaggtcc
    21361 gattagttgg aatgctgtag ttactattgt accaaatata cttattaatc cttgtgcaat
    21421 tggtgattta ttgtttatac tatctagtaa attatttact aagtttacta ctggaagtaa
    21481 tgaatctatt aatgctgtaa aatcgatatt ttgtaatagt gtacttcctg cgttaacaat
    21541 tgcttttggt gcattcccta tcattgttgc tgcatgattt tcaaaacgtt cttgcttatt
    21601 ttcaaccatc attttacgct tttcagcctc attagaaatt cgatattgag tagaatcaga
    21661 aaactctttt ccatttttta cacttctaga ttgcatctgg tcatatttgt tgccaataat
    21721 tatcttacca ttcattacat tgcctatttt accagccatt ttttcagata agccagctaa
    21781 attgcctaat ccactttcta taaaagtttt tattttcttt agtccttgac ctaaaatatc
    21841 atcaaaaggt ttaacaatac tcttcaagcc attgttaatg cttgtttgag ctcctgatat
    21901 tatacctggt attgtattac tcatttcttt agctaacccc atctgcttta cacttatgcc
    21961 ttccaatgct tcatcaaaag ttttataatt ttctcctacc agagtattaa gagattccat
    22021 gtttcctttt gctgcactta agaaagctgc tgatacatct tgttcactac ttgtattcat
    22081 atttaaagct ctaacatcta ccatagcttg agttatatct ttttcttttt tgccaccagt
    22141 aatattacta atcattttag ctttagttcc cattgcaaca acatcttcag ggtcaaaagg
    22201 cgtatcattt gccattctta ctaaactgcc ataataatca tcaacatcta tttgattaac
    22261 ttttccagta tttcttttca ttacactttc tagaacaact ttctgatttt gttcctgacc
    22321 aagtaaatca acaccctttt tcacagcgac acctatagta gcaccatttg tcaaatcaga
    22381 tatcttccct tttatcgaac caagtattcc acttgccata tcctttacag ctattgcacc
    22441 actccatacc ttacctgcaa ataatctcaa tttgggaaat actttactta taatgccact
    22501 tgccatatct tttatagcta ttacaccact ccaaaaagta tttttaaaca acattaactt
    22561 aggcaaaatt ctacccatag cactactcac catatcttta agattaatac ttccttgata
    22621 aggacgtttc tctacttgtt ttaattttcc caagatacta cttatcactc tcttagtcat
    22681 atcttttgct tttattacga tattccaaat cttgccacca atctttttta aatttccaag
    22741 taatttattt ttagtttttt ctacttcatc acttttagct ttaaatatta atataatcat
    22801 aatatttgaa ttaaactcat ttttcttttg aatcaactta ttaaaataat caataatcat
    22861 agctatagtt attgctagtc tcataatact tccatttaaa cttccaataa ctcctgaaac
    22921 agcatttatg ctagaactta attttgtcat agttgaactt acactactag cagtagtttg
    22981 aacttttctt aacgaggaat ttaaactgtt tactctatta cttgcacttc caatagaatt
    23041 ttttatgcta ctagccatta cattagtgga aagtgaaaca gccacagttg ctctcctaaa
    23101 attatcagtt actgatataa tttgattcat ttttggtgtg taattatctg taaggtacat
    23161 atttatacct tcttctctca catttcccat tacataccac ctcctgtcaa aggacacact
    23221 ccatatccat gtttcatttt ttcaacttct tcctcaattt ctaaggcgaa aaaagcttca
    23281 ataagcttta attcgccttt atccattgca taaaaaaggg acggtcttat accttttttc
    23341 ttccaataat agtacattat tgtagtcctg gtatccgtcc ctattagttt tttacctctt
    23401 ctatcactgc atttttacca taacccataa catcttgaag tattctatat aagttggtaa
    23461 tttcacctgg taataataat tttcttgcta attcttttgg tgttggagcc ttaaatttat
    23521 ccattagctc cttattttta aataaaagct ttccatccaa atcacaaaca ccttctataa
    23581 ttgtaagcat ctgcatctcc ggtaagtcta tatccgtttt catattttca tcaattttaa
    23641 gacaagtatt ttgtatttct tcatctttac ttattgttaa agctctacac attactttaa
    23701 attcctgccc aaatatagtt gataatctct taagttcaac tatagtactt ggtctttcta
    23761 agttttctgt atctgaattt aaaagtaaat ctactatgtt tatatttttt gataatccat
    23821 tttcactcat tattatttgc tcctttatca atcaaattca atcaactaac ttttttactt
    23881 attaactatc tgaatttatt aagtatatac ttactaaata tcagtataaa actaaatata
    23941 aaactaaatt atatcaagaa aatcatactc agtaaatgtg aaaggagctt ctatctctcc
    24001 ttttactcca acctcccaat cagctaaagt taaatcatca aatgctatat tttttactgc
    24061 tattctttct gctccataag aatctgggtc atttaatttt gatatagcaa caaatctagg
    24121 ttcagaacct ctctttatct tttctccaat taacttgtgc attcttgaac taacgtggta
    24181 tagagttatt gagccttttc ctttatatcc catatacttt gtatcagtac ccatttgacc
    24241 tgctattata atatcttctt ttgtaaattc catctttgct tgaaatttct ttacttctgc
    24301 tactttgttt ccatcaagcc aaagttctcc ccaagtacca ctcattacat ttctagcttc
    24361 catatttgcc atattaataa tcctcctata tttctattga taaatctata tcttccatag
    24421 catcaagtac ttttattttt gcttttaaaa atactttaga acctgtgtta gcttctttta
    24481 tttcttgtaa tgtcatataa gataaatcta ctccttttga ttttaaatac gatttttgtg
    24541 cttcaaaatc tatttcaaca gtagaatcag attctataag tgctgatttt tctaattctt
    24601 ctaaataact ttttatagct actatcaata aacatttgtt gtcataactg ttagtaacct
    24661 ttcctatata gtcatctatt atcacctttc ttatgtcact atgtataata tctaaagtgt
    24721 caactatttt tattttctgg aacatttctc ctttttctgc tgttaattca gttaaagaat
    24781 ttactcctct agcaattctt atagctcctg cttctttaat aagtataagc tctcctttat
    24841 taacccttga ttctgcatca actttcgtca tcttaggtat atcgactaca tcactaagtt
    24901 tagtataagt tactgattga cttaaaggtg tacctgctat aagtccagcc accctgcttg
    24961 taaactcatc aacactgtat ttcttttctc caactaacac atcttctgta gtaaaattaa
    25021 ttatcccttc atgatttcct acaacttttc ctaatacagc tttaacctta accttatcta
    25081 tatctctaag tttaattatc caatttttta tagcagtctt atcagcttct actgcttttg
    25141 gcatacatag ataattaaat tccttagtct ctaaaaaatc taatgcagtt tgaatatctg
    25201 cttctccttc tattacataa actaataatt tatttggagt gttaacattt cccatcaaag
    25261 ctaaatttat atattcttta ttttcagcag ataaatcaac tggtatatcc tctttttcat
    25321 gtatttcatt aagacctagt gccttagcat ctttaagtac cattgcaatt attcctctag
    25381 ctgaacgttc tttaacagtt gtagctagct ccttaaatga tatgttgata cttggtaatc
    25441 ctatagccat actatcactc tcctaaattt aaattgattt ctttcatatt ttcatatact
    25501 acatcttctt catatacact atccagatag tttagtgtaa atgtaaagtt caatacataa
    25561 attgactctt cttttttaat actttgttct agctttgtta tatttaattt tctgtctgtt
    25621 acctttatat ttctgttaaa tagcttattt aattcatctg aaatttcata cattctaata
    25681 ctctttgact gtggttttgg taagtatttt atagagataa caatatcttt gttattcgta
    25741 tttaacatta caggtgtaca aataactgat agtatactta caaaaaaaca agacccttca
    25801 gagccttgca cactttcatc atcaataaaa atactttcag taaagttttt tttgagaatc
    25861 tcaataattg tttctaatat ttccttgtat ttaagcaaat aatcaccaca ctatcactaa
    25921 atattcttgc ttaatctgat aatcttacta taaatgatat aagtcatttt taaatatata
    25981 tctcatttat tcctgtcaag tatcttctat atttttaggt aatattattc ttttcaatct
    26041 atcacctcct tcaatatgga tttctttcac aataacatta tctcacatat tttattgcct
    26101 attgttgcaa tggtgtatat aaagtgttgt aaaattgtcc tattttttat aatatctcac
    26161 tcaatttaaa tatcatcttt ttctttaaat tgtagcaaga tgttctatct aagtgtagtt
    26221 tcatagaaat atatgtcata ttgttttttg tcttactatt ataaaaaaga ttaaaaaatt
    26281 ccatttctat atcatttaga cacgttagag cattttctat cttctctttt tctatttcta
    26341 tatcttcttt ttccattttt aattttctta atctttcctc tctttttaac acctcatttt
    26401 ccacagaaga gcttatgtta tatgtgttac tagtcttttc tgtatatacc attgctccac
    26461 atccagagta ttcattctca catcttttta tgtccaagtt aatattcttt atctttatat
    26521 ccaaatactt ataattatgt aatcttttat ctgtttcttt aaataatata tcttttttgt
    26581 tttccatagt acttcccctt ttttatatac ttattattag cttcaaattt acaataatct
    26641 ctttagtgca cagtttttta ctaatctcac aactttcaag aacatattgt tctataactg
    26701 tataaaaaaa acttcttcta tattttgttc taaaatacta gaaatcttct tagccataag
    26761 aagtccagga ttttttatac ctctttctat ttgagatata taatcatttg taattccagc
    26821 ctttctggca agttgaattt gtgtcatacc tttgcatttt ctataatatt tcaagttatt
    26881 tttaaacatt gttttatacc tccattacta ttaagaatat tttgttcttc ctcacttaga
    26941 ttatatagaa ttatttgttc tttatcaata gattttagaa aaaaatattc ttttatattt
    27001 tattagtttt attttttaga acaatttgtt ataatctata cataaggtgg tgttataatg
    27061 tttgccaaaa gattaagaga acttagaaag gaatttggat tgactcaaag agaacttgga
    27121 gaaaaagtag gcgtttccca aagagtctta gggtactatg aaacagaaaa cagatttcct
    27181 gatgaacaca ttttaaataa attggctgat gtatttaatg tatcagttga ttaccttctt
    27241 ggaagaacct tagttaagga aaatattgat acagtagctg cacatagaaa aaatccacat
    27301 gaagagttac ctgaagaagc acaagaacaa cttaatgatt atatagaatt tttactaaat
    27361 aaatataaaa aaaaataaac catatgatga gcagtctatt tttctgctct tttaatataa
    27421 tttaaaggca aacatacatt ctattttagg gggattttta tgaataattt ggataagctt
    27481 tttgaattag cttctcaaga agagataata attcattata ccacttatat tgcaggtgat
    27541 ttagaaggac tatatataaa caaacatggt attaaaatta tatcattact cagtaattta
    27601 aaacaaaact caaaaaaatt gacatccatc ttagctgaag aacttggaca tcatttcact
    27661 agccttggat actatgtatc ctcttataac gattattata caaaaattat tatagataag
    27721 tgcgaaaata aagccttaaa atgggcttgt gagttcttaa ttactgaaga agatataata
    27781 aacataatta actctggcat cacttgcgtt tatgaaatgg cagacatact taatgtggat
    27841 attactttct ttcaaaaaag attagaattt ctatcattaa aaaaacagtc tttacaactt
    27901 ggaaataata aatatttaat attaactaat ttgccgtatt tctacatatt tgatccaatt
    27961 tcataataaa aatagtagta attttaactg gcttaacagt aaaatctata tatttatttt
    28021 aacaaatttt ggtttctata aattcaatat taatatataa taatgatatc atattattta
    28081 aaattctaaa aacaaatata tgcttttgtt aagaaaggtt gtttgatgaa tgtataaaga
    28141 aaagttgttt gataattatt ttaagttctt agcactatta ttttggccaa ttatgtggta
    28201 taagtggata gtaatttcaa atggtactct tgaaaatatg ttatttacca tatatgcaat
    28261 cgtcgcaatt gttttcatca ttttatattc ggtgtttatg ataaaatata aagacattac
    28321 acaaatagat ttcttttaca ggataagtac tctacttgct tttatattta cactttttag
    28381 ctttttaata tacccaaaga gtttattttt cttgtattta aagattattt ttactggaat
    28441 atacctttat tattcaatag taaaaactct taaatttaaa gatgacgaag gtgtagtggg
    28501 tattatgagc tcacttttat taattgtaat aactttgttc tactaaattt aattctattt
    28561 gacttaatat ggctttaaat attagacatc tacattattt tctgatatta tttaagatgg
    28621 tagtcttata ttaataatga aatacactat gaaggagtta actattatgg aaaaaaaagt
    28681 tttagcaaca gttggagaaa aagagataac taatattgat attgaaaatg cattaaaatc
    28741 attagaccct tatcaagcta tgcaatttaa aactgaagaa ggaaaaaaac acttattaaa
    28801 tgatttagtg aatcaagaat tattcttctt agatgctaaa gaagaaaaat tagatgaaga
    28861 agaaatattt aaattagaaa tgaaaaagat agaagaaaat gtattaaagc aatttgctat
    28921 aaataaagtt ttatctagtg ttaatgtaac agaagatgaa aaagtaaaat tctttgaagc
    28981 taataagagc tctttctcta aaccagaaag tgcaactgct aaacatatat tagtagatag
    29041 tgatgaaaaa gctaaagaaa tacttgctca aataaaatca gaagaaatat cttttgaaga
    29101 tgcagctctt aaacattctt cttgcccatc taaagatatg ggtggtgatt taggaacatt
    29161 cggtagagga caaatggttc ctgaatttga agaagctgtg ttctctatgg ctaaaggtga
    29221 agtaagtgaa ccagttaaaa ctcaatttgg ataccatata ataaaattag aagaccttca
    29281 agaaagtaca gagtctacat ttgatgaagt taaggctgaa gttgaaaaaa gtttattata
    29341 tcaaaaacaa aatgaagttt atggaaataa gataaatgct cttaatgcta agtacggaaa
    29401 ccttgtaaaa tacaacgatt aatcataagg tcatatataa aaaagctgtc ttttcacaat
    29461 atttattaga aaagacagct ttttatatta aagtttaaat attcagtttc atattatcat
    29521 tcttttattg taaagaactc agcttaaaag tttaaataag atttttaatt atcattagct
    29581 aaatttttat atccacgtat tatctcataa cagtaaccta ttacatctct tctttttatt
    29641 atcccaataa atgtgtcttg gtcatctata acaggaatga aattttgatt taaagatttt
    29701 ataactaaat cttcaatatc tgcgttgatg gaaacaggag aattatccat ttttctattt
    29761 atatctgtca ctggaacatc ttccaatccc tttaagtcca gatttaaatc attttttaat
    29821 gtccataaaa aatcaccttc tgttatagtt ccaacatatt taccatcctt acttattatt
    29881 ggaattgcag agtatttgtg atattccatt ttttctaggg cttgccttat agtgtagtct
    29941 tcatatatat atgctacttc actttttggt gttagaaaga ataatatgtt cattacacta
    30001 cccaccgtta ctgaaaactt ataaatatta ctcaatattt ataatgttac ttttttgcag
    30061 taactttctc ctttcttatt ttctaagtat tcttaatact atacgctgta acaagcgttt
    30121 ttcctttgct cattttaatc ttattatgct ttattaatat ttttatatgg tctatcagta
    30181 tcacttattc tctttttaga aaatttacta aaaattaatt gacttaaact tagccataat
    30241 aattttactc tacactatta attttaacat atttatctca ttattaatga ttttatttta
    30301 ataattttaa tttattttgc tattaatttt atttacaaaa actgaaaaaa ccgctaaatg
    30361 attagcggct tatctctcat attactagaa cactaatata tatgaatttt taattataat
    30421 ctaactacgt tagcagcttg aggaccttta gctccatcta ctacttcgaa ctcaacgctt
    30481 tgtccttctt ctaatgtttt gtatccatct ccagttatag ctgagaaatg tacgaaaaca
    30541 tctccttcac cttctacaga tatgaatcca aatccttttt cgttgttaaa ccatttaact
    30601 acaccagttt tcattaataa atcctcctaa aaaaacacaa atatttagct actcttttct
    30661 gtaaccttat tactactcta tcatatatag ctatgtttgt caattattat tatttttata
    30721 cattaataat caattttttt actttataat tttaaatgat tattatcttt tacactttat
    30781 ttttctttat aattttttaa taatttattt ttattatatg gaatcttatg gacttatcac
    30841 tccaacctaa atcccatggc accaacattc tatcagtatt atgacaagtt accaaaggat
    30901 accctcttga gtctaatcct gtcactgtcg aaacatgtgt tattctgcct ttcttctcat
    30961 aaccaacaaa atcccctgga cgtaaattat aagcttcttt gtatacttct tcataggaac
    31021 ctttagcaat taatgaacct cttccactat acagtgcata atttttaaaa gcttgtgcat
    31081 ttacccatgc ttttgtacct gctctctcgc tataattcca aattgagttc tttttgaacc
    31141 ttccactttc aaacattatc tgtgatgcaa agtttgcaca atctccacct tctggattat
    31201 aatccctata gtttggatta aatttcatac catgttcttc atcagctgcg gctccacagt
    31261 atttatgtgc ataatctata gccttttttt gttcatctgt aagctgtaac tctacaccat
    31321 cttgttgacc aatataattt cttatatcat cagattttat attttctaaa tttaaagagt
    31381 ctgcaaaagg gtctgtatac cactctttag ttatgacata ttgattatct ataattttta
    31441 catttagata atgatatgta cctattctaa aggcattttc tttgtccttt tgattttcat
    31501 atgaatactt atattcagtc gatactgaac agattatacc atagaggtct ttttcttttt
    31561 cttttatttt tttaatctta acccttgtct ttatgtcttt aaattttaca ccttgcttac
    31621 tagaccaatt ttctaaatac ttcatctttt ttacttcatt ttcataagcc cacagaccaa
    31681 attttttatc cgtctcataa agacctttta aaatgttttc atttttctct aatattgcct
    31741 tatttctgta atcaaataag ttttcaagca atagtttata ttggtctttt acatcatcac
    31801 cttcaacatt tgcagattcc atgacttttt cttccatatt tttcatactt aaatcattga
    31861 ttaaaaatac acctgctgta gtagctaaag acaaaaatac aaatgccatt atacctttga
    31921 tgtacttatt cttatttttc agtattttca aagctacacc tcccaaatag tacaccttac
    31981 aaatcaaatt acaaagaata ttctgtcaca tatagtaatt ttcatattta ataattctct
    32041 attttattat gtccaatatt aaaaattaat tgtatcaata cactgttttt tttacaattt
    32101 tttattttat aatctacttt tttctttttt ttatagtaat atatatgtat ataaatattt
    32161 tggtatcatt ataattaata catactaatg gagggttttt tatgcaaaaa aaagtcgcag
    32221 caattaatga cttatctgga ataggaaaat gctcattatc ggttgctata cctatacttt
    32281 cagcattaaa ggttcaatgt tgtccatttc caacggccat actttcaagt caaactggtt
    32341 atcctgaatt tactttttta gactttaccg atgaaatggt aaaatattca aatgtttgga
    32401 aaaatttaaa agtcaatttt gatagtatat atagtggttt tttgggttca aagcatcaaa
    32461 ttgagatagt ggctaatttt ataaacgatt atcccaatgc ttttatcgta gttgaccctg
    32521 tgatgggtga taatggagtt atgtatccaa tattcacaga agaaatgaga catgaaatta
    32581 aagaattagt taaacattca gatttaacta ctcctaattt aacagaagct tgtttcttaa
    32641 ctggaaatga ttataccaaa agcgattata atagagatga gcttatatat attgcaaaat
    32701 ctgtatctga tttgggacct agcaaagtcg ttattactgg tatattagaa gatgataata
    32761 tattaaattt ggcttacgat agagataatg accatgtatt ctttacatca gtcaaatata
    32821 ataattgttc ttatagtgga actggagata tattcacatc tatactttgt ggtatgttag
    32881 taaataaaca tgatttggga gtagcggtta atactgcaac agattttata tataaaacta
    32941 taaattacac atctcaattt gatactgata gaaatgatgg agttatgttt gaaaactttt
    33001 tatcagattt aactaatata taaagtcaaa aaaactatcc attttataaa tacctataaa
    33061 tcataaaatt taggtattta caaaatggat attatttact aaactattgg tatttcaatt
    33121 gttaccatag caccaccagt tttcacagaa ttttcaatat ataaaatccc attatgtttt
    33181 tgtacaatat tatttacaat atagagtccc attccataat gagatttaga atttctactc
    33241 aaatctcctg tataaaactg ttctgtagct tttgacaaat ctgctttaga aaaaccactt
    33301 ccacaatctg taactacaaa ttttaagtat ttattacgta ctgatacact tatgtatagt
    33361 ttactctggc ttggtgaata atctatagca tttgaaatta tattcataat agctctgaat
    33421 aacagttctc tatctatagt tatattttta ggcaaattat cttgtttata aaactccaca
    33481 tctaagtttt tagtactagc taatgcttct atttgcacta agacatcttt tataaactca
    33541 ctcatactga ttgcttcttt atttaatata tagccattct ctgtttttga tatatctatt
    33601 aaagtattaa tatatttttc tatttgagtg acaccctttg agatatattc catgtattct
    33661 aataactcag gattttcatt tatttcaatt aataagtcag tatttccatg tataattgtt
    33721 agtggtgttt ttatatcatg tgccagtgca gatatctgtt cctttctatt ttcttctagc
    33781 atccattgtt tttctaaaga atcttttaac gccaacttca ttttatccat agaaaaaagg
    33841 acattattaa tttcataaat tttactagat tctacaacaa aatctaaatc ctgttgttct
    33901 attttttctg tagtattttt taaaagctcc atttcaatat taaatttctt gccaaatact
    33961 tttgataata taacaatttc aataaaaaat attatactaa aaataatcat acccaatgtc
    34021 tctggttttg gtaaatatgc tcttaaaatt ggagaactat actctgatac tattttgtat
    34081 tttattataa aaacttcatc tttcttaaaa aactttatat aactttctga atatgcatgc
    34141 ctttcttcaa tatctctcat taaattccat acttcttttg attctttttt attaaaggtc
    34201 cctgaaatta aactcccatc caaagtataa accccatact catatatact tggaattata
    34261 tcttcagtta ctttttcact tgaaataatc tgttctttat atttattaaa ttgtttttct
    34321 gcataattag ctggtaaaat tacacctgat gataataata cagaaaatga taacattaaa
    34381 aggagtacaa ttgaaattgt cataataaag aataaagata aatattttat aaaaatagtt
    34441 cttaactttt caactttttt gttgttagat tttttacctc caagcatttg taccctgtcc
    34501 tccaatagtt taattaattc ataattaata tctaaccagt tttttatttg attattttat
    34561 atctttttat tgctaagtta tattttaaca taataatata aataaacagt caatttttac
    34621 tctattcttc tgatttttta ccttcccatt tagaaaacca aatacataaa attacaaatg
    34681 ctaaaattgt tacaaaacca cacattaaac ctgttgattg atatgctttt attgattcaa
    34741 cctttatata ttcaaccgtt ttagaactcg aaaaagacga ccaaatagtt ataaatctaa
    34801 caccccatgc acaaggaaaa tactgccaaa taccagaacc caatccagtt aataataatg
    34861 ctgaaagtaa catttcaact atacctactc caattgatgt tccttttccg aaccttaaac
    34921 ttaagaataa atgtaatatg tattcaaata aattacaccc aattaaaatt agaattgaaa
    34981 tcatataaaa gcttgatgca aagtaagcct tagatatacc aaatttaaaa cctaatacag
    35041 acactaatgt tgctacaaaa ccaagacata ctaagattat atatttactt ataagagtaa
    35101 gatattttgg attagatgtt gttaacatat gtttataatt ccctgcataa tattcttgtt
    35161 caacaaacat tgaacacaca acacctgaca ataaaggaaa agctattgct ataacttgta
    35221 aatatgctac tatctttagt atattatcag ctcctgaaac cttataatac cataaaaaca
    35281 atcccatccc cattaaagca atgcaaatgt gcattagtat tataaaattt ctctttaatt
    35341 ttattaaatc agaacaaaat attcttatta actgtctcat attacttcac ctctctatta
    35401 ttgaaccaaa cccttgtagc taaagcaaat aatacaaata aaacaataga tattaatact
    35461 cctggcaaaa taacagcatt atttaaaaat tcacttccct ctggtacagg aagaccattt
    35521 ggcagaatat gcaatacaac agccattaat cttgaaggaa tcgaatatgg aaacatatac
    35581 cacatttctg tagttgagaa aacagcaact ccaacaatag taaagaacat atttattaga
    35641 actgtaacaa atataccaaa ttttgaagct aaaaacatac aaagaggaat ctgccacata
    35701 aatagtaaaa taattaatat agttgcaatg atattttgac ctattgaaat agtttcttta
    35761 tagattatcc caacaaaaac tacacctatt gcagaaataa ttgatgctat aaaacaacaa
    35821 ataatacagt aaatcatttt tccagtccaa acgtctttca tttcaattgg caatggtaaa
    35881 ataccacgat atttactttt ttcatctttt aatactatta aagaagatat taatgttaat
    35941 gctcctggaa gaaatgtcgt ataccaccag ttgaaagcag cattttgccc tccacctagt
    36001 ccacataaaa gtatacaaaa cactggaact atccatatca acttcattcc aaaagttcct
    36061 ttcatctttt gaaattccac tctaataaat ctttccatat tactgccctc tctttctatt
    36121 ttcagctaca actttcataa aaagtttttc taagttttgt tcttttgata cttctccttg
    36181 ataacccatt actccattag aaattattcc tatttcatct acaacttgtt caacttcaga
    36241 caagatatgg cttgataaaa taactgtaat tccttgtttt ggaaaagaac gaatcaattc
    36301 tctaagttct tcaataccaa tagggtcaag accatttgta ggctcatcta agattaaaag
    36361 ttttgggtga tttaataatg caatagcaat accaaggcgt tgcttcatgc ctagagagaa
    36421 ttgacctgaa cgctttttaa aggtatctct taaatctaca gtctctaaaa cttcttcaat
    36481 gcgagagtca ggaagaccaa gtaaagtagt atggactttt aaattttctc gtgctgttaa
    36541 attctcatat atagcaggag actcaataag agaaccaata tttgataaat cctttctagt
    36601 ccatctatgt ccctcaaaca ttatttttcc tgaagttgga tttaaaatcc cagttatcat
    36661 ttttaataaa gtagatttcc cagctccatt tggtcctaat aatccatata tagaattaga
    36721 tttaattgct agtgaaatgt tatcaactgc catctgctcc ttaaattttt tagaaactcc
    36781 ttttacctgc aaaatataat tactcataat tttcatcctt tcttttgtca aacttattta
    36841 caagaatcat tctaaatcat aaatataagg attttataag aattaaaaaa caaaaaaaga
    36901 tgtgttaaaa acacatcttt ttttatctta attctattaa ttttccattg tagtatatct
    36961 ctacttcttc aatccaaatg ctattatctt cttcttggat gacattttta ataatattat
    37021 ttatagtata tattatattt tgttcattca aataatcatc tttccaagaa actctacttt
    37081 ttttatctgg ttctatcaag tttattttaa caagattatc tttttcctta acttctactt
    37141 ccatgtctaa accattaaaa tactcatttg ataatgtatc taagattaat cgcagtttat
    37201 ccttaagtga actttcttca tcaatctgta agtactttac tattttctca tcctttgaaa
    37261 ttatctctat aggtatttta gtagtttttt tagaattaat cattttacta gttgtttctt
    37321 cttttgcatc ttcatccttc tttacaactg ctgattcttt agaatcaact agctcaactg
    37381 cactgatgct ataatccata catgccgtac tcaataaagt agtagcaatt attaaaaaac
    37441 ttactgctat tttattcata agtatccctc tcctaattct ctttcgatac ttattatacc
    37501 atagtataga ttatattttt atacttttaa atcaatttat aaattattat agtaaattat
    37561 ttacaattct aagctatttg aatttatttt tatagtatat tctttctcta aattcgacag
    37621 atttttattc aaaagccctt ttatttctct cgtatttcta tacttgttgc tatgttcttc
    37681 actatgttca taatcatatg aatcattgtc atattcttca tcattattaa attcttcaat
    37741 tgcttctttt gactttatat cacaattaaa tttatactta tttactggta tatcaagtct
    37801 taccttagaa tttggaactt ctatgtccag gaacttagca attggcatat cagagtacaa
    37861 gtaaaattca ctttcataat ttgaatttct acctattttc tcttttatat ttacattttc
    37921 aggtatatac tcttctatat tattgataaa tacatcttca ttctctgaag atatatacaa
    37981 tgactctgat tctatgttta catttttaac gcctaaaatt gattttgttg acaagtgaag
    38041 attgttatta gatattaagt ttaggttttc taatctattt actttgttct ctgttatagc
    38101 tctactaaaa cctccataag aagttttata tgttatagta tttgaagcta cattatcgta
    38161 tacaaataag tgtgagctta tacttgatgc atttatgtct acattatttg gtacatatat
    38221 tataatttct ctataatcat gtgaaaacat actatttata gatgtattta ataattggtc
    38281 gaaattttta atctttttaa ttttattttc atattttctt tcacctttta caactaaagt
    38341 cttatctttt aaagttacat tgtatttgta aaactcatta tttcctcttg ttgttacacg
    38401 cacatcttca cttgaacttt tctttattgt aacgtttata ttgtcaacag ctatatctaa
    38461 cttgtcaata tttacttttt cttgataaag tttattttct tttttaaatt cagcttcctt
    38521 ttctaaacca aagtttatta ggcttggcat aactaaaatc cctgaccatg ttgtccctat
    38581 tatacctatt accaataaaa ccgcagcaaa tatagcaagt tttttattca tgtttatctt
    38641 ctcctttctt attttctata tcttcaaggt ccttatatat cgcctcaaaa tttatactat
    38701 catcattata ttcatcagtt tcttttatgc tttcttcttc tctattgtag taatcattat
    38761 tatatgaatc ttcattaatg tctttattca aatcttcatc atttaaagta gctttttttc
    38821 ttttttccat cttttcattt gcttttatat aaatattttt tctctttatc caatgtatat
    38881 aattatttag taattgttta cctagtttta tggcaaataa taaaatttgc catgcaagta
    38941 tctcaagccc tacaaatgct atggttgcga aaatacaaaa catcataaca tctggaacca
    39001 ttgctataaa tttaatcaca aatggaatta atataacaaa agcaattaat gagcatacta
    39061 atgcaatagt agcagataca atagcacata caaacgcaaa tgctggaaca attagaaata
    39121 aagataacac tactaataac ccctttataa cttgtctgga taatcctcct acaccttctt
    39181 ctatacttgg tgtaagttta tcatttatat tttcttttga tttattgtac ttttccttag
    39241 cttcatcttt caagatagaa taatcctttt ttacatttga tttaaatcta ctaaatttac
    39301 catttaatat gtctgtattc attatttttc catctatatt ttcttttgat ttactatact
    39361 tttcttttgc ttcatctttt aagatagagt aacctttttt catatctgat ttaaatttac
    39421 taaacttatc attcaaaaca tctgtattta ttattttttc ctcttcaata tttttttctt
    39481 tagtttgtaa gataagttct tttgctatag attttggtga gccaagtgat gctattatct
    39541 ccatatcact ctttccctct ataagaccat ctacaaaata ctcttcataa tctctcaata
    39601 tatcattaac ttcgctctca gaaaaatctt tttttagata atcctttaaa atttcaagaa
    39661 actcagtttt attcaagttc attacctccc tcatcttcac ttattaaaat ctcaacagcc
    39721 tttacaaatt ctctccactc ttccatcaga ttggataaat tctcttttcc tagtgaagtt
    39781 attctatagt attttctagc aggaccttca ttagattcta gtatatatgt ttcgaaatac
    39841 aaatcttttg taagtcttct tagaatagga tatattgttc cttcatttac atctattact
    39901 ttagagatgt tatgtactat ctcatatcca tacatatctt tcttagatat gagagcaagt
    39961 acacaaattt ctaaaacacc ctttctaaat tgtgtgttca tatactccat cctttcttta
    40021 aatacacagt attgtgtttt acaaggtact atgttaaaac ttcttatatc ctcataataa
    40081 cacagtacta tgttctgcac aatagttatt tttaaatatt cttgttgatt aaatccttct
    40141 ataaatgtat aatactctaa tcagatatat tttatgaaag tggtgataaa ttggctaaaa
    40201 tgaaagatga taaaacaata ttaatcctaa ctgctcaatt tggtgctgga catataagtg
    40261 ctgcaaaagc agtcaaagaa tgtattattg aaaaatatag taactataat gttgttatac
    40321 aaaactttat aaatgcaagt attcccatga tgaataagcc tatggtaaaa ctttatgaaa
    40381 acaatacaaa atatactcct ggattatata attactatta ttactttaag aaatcgtttg
    40441 attcaagaca tgatttatct cataaattat atacacctaa actttctgag tatattgctg
    40501 atataaatcc agatttgatt atctctacat ttccactagc tgctgcttgt gtaaataact
    40561 ttaagataaa aaatcctgat ataaatatac ctacacttac agttataaca gatgttgtag
    40621 atagtatgga atgggttttt gaaaatacag atttgtattt tgttccttct cctgaaataa
    40681 aaaatagatt ttttcagaaa ggaataaatc ctgactccat aaaggttact ggagttcctg
    40741 ttgataagag atttcaaatt gaaagtaaag aactatgttg tgataaatat agactattac
    40801 ttctaggtgg aggaagaggt ctatttgata tagatgagga ttttatgcat tggatagatg
    40861 aatttattga agaacatagt aattctatag aaattacaat agttactggt aaaaacaaaa
    40921 aattatatga caatctaact cataaaaaac ctctaaaaaa tattaaagta cttggatttg
    40981 taaacgacat gtataatcta ataagagaat gtgacctaat gctaactaag cctggaggag
    41041 caactatatt tgaagctatt caatcacaaa ctccagtact cgtaaaaatg ccaaaagttg
    41101 gacaagaaat tgaaaacgct aaatttataa tagataaggg tcttggcatg atttatagtg
    41161 atgacctaga ccttaaaaac atattttata aattagtatc aaatgaattt gattctataa
    41221 taaactttat gaaaaaaaat ttaaaagaat ttaaaacagt cattcatcca gagaaaatta
    41281 ctgattatat atcagaatta atagataatc attacagtta aattgtacta ttaaaaaatt
    41341 tcaaacataa aattaaaggc aatcaagtat gattttacac ttgagaattt actcatagtg
    41401 tttaataata cttgattgcc tttatcatat caaaatcaaa ttgttatatt agtatatatt
    41461 tttcttgtac tgcatataca gttgttgtat ttcttccata cttctcttta attctatttg
    41521 taaatctgaa gcagtctggt aatccttatt ttctatagct tctctcactc tagtgaatag
    41581 tgattctcta gttaaagtat ccttcataat attgaacttt aaatccgata ttgagttcca
    41641 aactacctca tcaagcgcat ccatattttc ttttgtgtgt atcttgacaa tacttcttat
    41701 tttttgaatc ccagcctcta ttattctagt ttttaatttc ttttgccact tatctatagc
    41761 tccaatctta aatgattcta ttatactatc agtaaataca tcaccttgtt ttaaagattt
    41821 taattttgaa gcatatattt ctaagttttt catattttca taaactgtag ctggtggtat
    41881 tccaaatctt gcatttcttt cttccaaact ataatgttca aatacatcat tttcatctct
    41941 atatgctcta tctttttcta aatagaaact ttcttcacct acatttttag ataactcttt
    42001 ttcaagttct tttgtactaa gaccactctt agctgctgct tttataccat ccaacattgt
    42061 ttgataacat cctgctatta ctaaatacgt gttagataga gggcttggtg accttaattc
    42121 aaatcttaca gtctttggat ttttcatatc tcttattaat ccaacaagca ctgacctatt
    42181 tcttgatgga atctcataac tatgccctaa agatgtaact atacatacag gagcttcaaa
    42241 tccaggaact aatctattaa atccatcatt agaagctgtt acaattgggt ttaatacttc
    42301 ataattgtaa agaagtccca taagagcccc atatcctagt tcacttagat aatcttcttt
    42361 taaatctttt ggagcaaata gattttttat acttccatcc tttaacttcg cacttactcc
    42421 tacatgtgta tgtccaccac ttcctgctac tccatgaatt ggttttgctt taaatgtaac
    42481 ttctagtcca tgtgatgtaa atacatcctc tataacatct cttactaata attcattgtc
    42541 tgctgcttgt aatggtgtag aaaacttcca agaaacctct aattgctcca tagcatggtt
    42601 tgttcttcca tctatactaa tagaactttg tattcctccg acctctttgt gagccatttc
    42661 agggtttaca cctagctttt gaagtataat taaacttttc tctaaacaag ttcttataat
    42721 accatgagtt cttttccagt attgctcttt taggctttgt gatacatata atttctctaa
    42781 gtcagcttta tcctcaggag tattaaccca gaactcaagt tctgtagctg ctgttaacat
    42841 tatttcctct atatcatcta ctgaatctat tcctatgtta tttattacat gtggatattc
    42901 tctaaatatt tcatacattg actttttaaa gtacttatct gctttttgaa gtactcctct
    42961 agaacatact tttttattat catgtattaa aaatgcaggt atctttaatg ttccaacagg
    43021 tagacctact tcttcgtcta tatattccat attgtaatct acataccaat gacaactttt
    43081 atctggcatt aaatctactt tcgcattatt taatgtagct atattataaa gctcaacact
    43141 tgaaccatca gtctgtatag ctgattccaa aaatttatct atatcctcca aaaataattc
    43201 tacaggaatc ttttcatctg ttgcatttcc tcctaaatca acccccatta aggaaacaaa
    43261 tcttatattt ttattgcttt ttaggatttc tcttaattct tcattagtgt gtttatcttt
    43321 ctctattacg tataatagac tatccatttt tccactctcc cataattttt atgtatattt
    43381 tattttgtta aatacaagat aaatttcatt ttccttacat ttatgaattt ttcttgcaaa
    43441 ttattcgtgt tttattcgtg tttttattta tattattata tatcaagtat aaaaagttcg
    43501 caatattttt tattggaaaa aatattatac atacaatagt ttaacatttt tacaaatatg
    43561 gggtatacta aaataaaagt atttttattt gaaaatttaa ttttatttat aatataatct
    43621 atagtaaaag aaataataaa aaaatttagg agataaactt tatggaatac ataattttcg
    43681 atttggaatt taaccaagga tttgataaaa aactcaataa aactgtatcc aatgaaaagt
    43741 gtccttttga aattatacaa ataggagcaa ttaaattgga ttctaaattc aatataatag
    43801 atacgtttaa tagttatgta aaacctacaa tttataaaga catacatccc tttattagtc
    43861 gtatgactaa tattaaaaat tctgatttta atgattcgcc tactttccct gaggtctaca
    43921 acaattttat aaaatttata tcatcacaag accctatatt atgtgtttgg ggagctggag
    43981 atttaaagga attatacagg aatatcaact atcacaagct accatcaaat tccttaccta
    44041 aatcttatat taatatacaa caacatgcat ctaaatattt caataatcca gcaggaaaaa
    44101 gcattggcct acaaaatgct atttctatat tagaacttga tgaaaaaatg tcttatcaca
    44161 atgctttaaa tgatgcatat tatactgcta aagtttttat taagatatac aatccaagta
    44221 tagtcccaga tatatatctt tatacaagta taaaacctaa aactataaga tattctaaca
    44281 aaaaaagagt tgattatgat aagttatttg atgaatttag gaaaatttta aatcgtgaat
    44341 taactaaaga cgagaaaaaa atcattaact tagcttataa tatggggaaa acaaatcaat
    44401 ttacattaga aaatgttaag caaagaaaaa acaaatagag tttatttaga taaaaaatag
    44461 gcatgtagat taaaaaacta gttaacttta aatttatatc tacagcccaa tttttatcat
    44521 aattaatttt tattttatct ctaattcaac tggacaatgg tctgaaccta atatttcagt
    44581 gtgtatatcc gcacttacta atctatcttc taattttttg gatgcacaaa aataatctat
    44641 tctccaccct gcattatttt ttcttgcatt aaatctatat gaccaccaac tataaacacc
    44701 ttctttatca ggattaaaat atctatatgt gtctataaac ccactgtcta ataatttaga
    44761 aaacttctct ctttcttctt tagtaaaacc tgcatttttc atattatttt taggattttt
    44821 taaatctatt tctttatgtg caacgttcat atctccacac acaattacag gcttatgatt
    44881 atctagtttg gttaaataat ctataaagtc gtcttcccat ctagttctat attctagtct
    44941 ctttaattca gattgagaat ttggcgtata tactgtaaca aagtaaaaat cttcaaactc
    45001 tagagttatc actctaccct ctttgtcatg ttcttctata tttattccat acattacttt
    45061 taaaggctct ttttttgtaa atatagcagt acctgaataa ccctttctct cagcataatt
    45121 ccaatattga aaatatcctg gtaaatctaa ctctatttgc ccttcttgca atttagtttc
    45181 ttgtaaacaa aatatatctg catctacttc tttgaaaaag tctaaaaatc ctttcccaac
    45241 acaagctctt attccattaa cattccatga tataaatttc atacttatac tcctcatcta
    45301 aacattgaca cactccccat agctaaagat gggggctttt caacaatttt attgtaaatt
    45361 cttaattcac ttttatttta acaatgctat actataattt catctaaaat tactatttac
    45421 ttaataccta aaaatatatg ttttaatcaa tttttaactt ttttgttaaa tcttcagggc
    45481 tcattgaaga aacatacata tttgttccaa gttcaaatcc tccaaaatta tattttcttg
    45541 ggattatgtg catatgaaca ttaaaatacg attttgtttc aatctttgtt ggatgagtgt
    45601 gaatacataa attaaatggc atatatccat tgatattata taacttctta aacaactttt
    45661 caaatattgt agaaagctcc tgtatattat tatcatttaa ctcaccaaac tctattttat
    45721 ctttaaatat tattcttact tctccactat acttggttgc atatggtata agtaccaaaa
    45781 aagcttctcc attataaaca actcttctat ctaaacttat ttcattttct attaaatcat
    45841 catacaaaga gcttttattg ctgtcataat actcctttga aaccaacagt tcattcgtaa
    45901 tttcaggtgg aataaaagac atagacaata tttgagcatg tggatgcatc aaagatgctc
    45961 ctgcctttct caagaagttt ttgaagatac taacatactc tactttatca tctttcgaca
    46021 aatctctaaa tctatttcta tacataaaaa aaacatcttc aaattctttt tgactcatgt
    46081 tataaaaact acgattatgt ttatcgctct ctatcattac ttcatgaaca ccatatacat
    46141 ctcttgccat atcatcaatt ataggaaatt tattgtagac agattttaca agccatcctt
    46201 cttcactttc tatttcaaat aatgcatcag ttgcatgagc ttcatttcct ttgcaaaatg
    46261 ggcattcttc atcatattca ttagtttctt catcttcatt tgggatatct gctgtatcta
    46321 aaggtctttt taacctatct gtagcaaata taacaacatc attggtaatt ggatctattc
    46381 ttaattcttt cataattaac acctcttagt taatagcctt taatataatt atatgatatt
    46441 tatcgtatct gttaaataaa tttataatat tttttataat tttgcatatt tattattata
    46501 tattttgtct atagctgaaa gattataact aaaaaatcac tatcagtata tcagaatatt
    46561 atcttactat aaattaattt atgaatacat aatttagtgg cataaacatg ggtattttac
    46621 tataatttgt taaaagttta ttttaaagct atattaattt tttttgatga tttttaccca
    46681 taagtatgtt attattatct tgtaaagatg tcttgtattc aaacatacaa tttacataat
    46741 acaatatacg tatttaaatt aaataggggg tattaaaatg aattattcaa acagagttag
    46801 tgcaatgcaa gcctctccaa tcagaaaact tgttccattt gcacaagcag caaaagataa
    46861 aggaatcaaa gtatatcact taaacatagg acaaccagac ataaaaactc ctaaaggatt
    46921 ttttgatgct gttaaaaact ttgatagcga agtcttagaa tatgctacat cagaaggtat
    46981 tccagaatta ttagaagcac ttcaaaatta ctataaaact tacgatatga attttgaaaa
    47041 agatgaatta ttagtaacta atggtggtag tgaagcttta cttttcacta tgatggcagt
    47101 ttgtgaccca ggagataatc tattagttcc agaaccattc tatacaaact acaatggatt
    47161 tggtcaatct gtaaacgtag aagttaatgc tgttacaaca aaggcagaaa acggtttcca
    47221 ccttccatct aaagaagaaa tattgtctaa agtagatgat aaaactaagg ctataattct
    47281 ttctaaccca ggaaatccaa ctggtgcaat ttatacaaaa gaagaactta atatcctagc
    47341 tgaaatagct aaagaaaaag atttatggat aatagcagat gaagtttata gagaatttgt
    47401 ttatgatggg cttgagtata caagctgtgg taacttagag ggtgtacagg atagagttat
    47461 aataatagac agtgtttcta agagatacag tgcttgtggt gctagaattg gttctatcgc
    47521 ttgcaaaaat aaaggtctta tcgctcaaat ccttaaatta tgtcaaggaa gactttgtgt
    47581 tcctacatta gagcaaatag gagctgttga gttatacaaa actcctgttt cttacttcaa
    47641 agaagttaat gaagagtaca aaaaaagaag agatgtatta tacaatgaat taatgaaggt
    47701 tgaaggtgtt atatgtaaaa aaccaactgg tgctttctat atagttgcta aattaccagt
    47761 tgaaaatgca gaagatttca caatttggat gttaaaagaa tttaacaaag ataatgaaac
    47821 agttatggtt tgccctgctg aaggattcta tgctactcca ggtttaggac gtgatgaaat
    47881 aagattagct tacatcttaa atgaaaaaga ccttcataga gctgctacat tattaaaaga
    47941 aggtttagag caatatgtag ctttacaaaa ataatttttt aaacttaatt tatataaatt
    48001 taatgatata aaatgtcccc ttttcagata ttatttgaaa aggggacatt tcttaattaa
    48061 tatattaatt aagacttaga cctgactatt tttcattttc tttatatagt gttggccagt
    48121 aatctttatt cactttaatt aaagcatcta aaacttgtct tgccttagtt ggagagatta
    48181 tagttctatt taatgttaaa gataaaatag ctttttcata agattgttca aagtatgcat
    48241 caactgctaa cttttcataa gctagttgac cttctaacag tcccttataa aatgttggta
    48301 tattacctac atgtaaaggt ctcggtccat tctttgtaag aatcgcagct acttctacca
    48361 ttgcatcatc aggtaagttg ctaatagccc cattattttc tgttataact atggctattt
    48421 ggtgtttgtt atgagctatt gcttctgcaa cttcaactat aaattcccca tgagcatcat
    48481 tatgcactaa attagaacct atagatgttc ctttttctat aacagattta cattcttcaa
    48541 atacacgctt ttctcttcca ttcataactt ctcttgctct agtacaattt atatttgatt
    48601 tagctacttt tttatcagga tatagataat actgtacata tgtattaggt aagtaatctg
    48661 gtacatcttc caacatatct ttaaccattc cataagtgtc taaccaagat ttatctcttt
    48721 gttctgcatc aacaggttgg aatccagagt ttgctactaa atcttttatc ttaggtacca
    48781 aatcttcacc atttttatca tacaaatgtg taaaccatcc aaagtgattt aatccaaagt
    48841 atacaggctc aaatgttctt gaatccatgt ctaacaatct tccataagac cttaacaagt
    48901 taactggttg gtcacacata ttaagtaatc ttttatcatc tggaaatact ctttttaatg
    48961 cttctgcaac aatagcagca ggatttgtat aatttaatat ccatgcttta ggagagtatt
    49021 ttcttacagt cttaaccatt tctatcatat cacctataga ccttaaccca taagcaaatc
    49081 caccagctcc acaagtttct tgaccaacca gtccaaattg tagaggaatc ttttcatcta
    49141 attctcgcat cttaagacca ccagttctca tttgaacaaa tacaaaatct acatctataa
    49201 atgcttcatc tgggtttgta gtatattcaa gtgttgcttt aggatattct tctttaaata
    49261 atatttttcc aaattcccct attaatcttt gacgttcttc tactacatct aacatagtta
    49321 ctttatttag aggaaatcta tcactcattt tacataaaga ctttaaaagt cctggagtcc
    49381 aagtactgcc tcctccaact atagctatat tgtatctttt tttcattgtc atattaatca
    49441 cccctgtttt ttattaacta acttgtaaga tgattataat atataaagtt ttctacatga
    49501 atacttttag gtaatcatta aaaatagttc aattttagta tttttttcaa aattgtaatt
    49561 tttttcatct attgtatttt tcaatgtatt tatgaaacat atattccata agagaaagtg
    49621 ctggcactct agatgtatag ttaacactat taaaattata ttgtggagaa gatacaaata
    49681 attttatatc agctatattt gataatgaat tgacacttat atttgtcaga gaaattactt
    49741 taacttgatt tgtttttgct atattacatg ccttaataac ttgagcagtc tctccagaca
    49801 tagatacagc aattacaagg tcatctttag taatatttga tgcagccaaa tacatgagag
    49861 ttgaatcatc atagtatttt acatttacac ctatagctga aaaacgttga gacatttcag
    49921 tacatactaa tctagaagac ccaacaccaa aaaaatatat atgcttagaa ttatgtattt
    49981 catttaaaca catatctata ttctctacat ttattaaatt ttgtgtatcc ataacatcag
    50041 taaaaatatc actttgactt ttagtaaact ccttatttct ttcatttcta tttgaatatg
    50101 aaaaaactaa aaagttttta aattcactat atccagaaaa gccgagcttc tgacaaaacc
    50161 ttactatgta tgcagtcgaa ttaaaagttt catttgctat atctttaatc tttttttgat
    50221 ttattttttc agaatttttt agaaaatagt ttaaaagaga tatttcattt gtagataatt
    50281 ttttttgatt tatatctaat ttgtctatta ggttcattta tacctccata ataatgattt
    50341 gtagttaaac aactttctca tatttattat ataaatatac actatacagc atttatctta
    50401 tgtttattat actataaaaa agacaataca aattataaat tagtattgcc ttttaatatt
    50461 tactttaaat agacattgca aaagaaactt cgtctcttac ttgagataca gatggcccat
    50521 aaattatttg gatatcttta tcagatactt ttataactcc cattgcacct gtatattttt
    50581 taaaagcatt ttcatctact ttactcatat ctttaactac tactcttaag cgagttatac
    50641 agtttgttat atcttctata ttgtccttac cacctaaata tttaattata gataatgctc
    50701 tcttagtagt ttctccactt tgagctattt ctactttatt tgataaaggt tttaaatcaa
    50761 aatttatatc tgttatttct tctcgtcctg gagtttgtaa gttaaatcta agtatcaaaa
    50821 tcttaaatac attataataa actattgcat acactaaacc aagtattata gaataaatcc
    50881 actttgtatt tggttgcata actccaaata ctactaaatc tattatgcca ccttgagtat
    50941 ttcctattgc tactccaaat atatgcatca tcataaatga tatacctgat aaaaatgcat
    51001 gtataacaaa taatattggt gatacaaata agaatgaaaa ttccaatggt tccgtaattc
    51061 ctgttacaaa tgttgctaaa gcaccagcta gtaatatacc tttaaccttc gacctatttt
    51121 caggctttgc agttttatac atggcatatg cagcagcagg aagaccaaat accattatag
    51181 gtattttccc ttgagctaaa aatctagttg cttctgttga aaaactgatt gtttcagggt
    51241 cacttagttg agctaagaat atgttcaatg ctccaaatac ttcttgccct cccacaacta
    51301 aagaacctcc tacttcagta aatctaaaca tagcatttaa tatatgatgt aatccaaatg
    51361 gtattaaaag tctttcaaga aatccaaata caaatgttcc taaatatcct aaactagcta
    51421 taccaatacc tacactctga ataattgtat ttacatatgg ccatatatat ggtattatta
    51481 tccccaaaat agagaatact actgttgtta ttattggaac aaacctagtt ccaccaaaga
    51541 atcctaaata atctggcagt tttatattat agtatttatt atgtaatgat gctgttatta
    51601 taccaactac aattccacca aatactccta attctaatgt ttgtatgcct aaagttacag
    51661 cttgacccat ttctctcatc atgtcaggtt cagcaataat ccccctacac cctaacatat
    51721 aatttacact agtgtgtaat actaaataac caacaaaacc agaaaatgca gaaactgctt
    51781 tttcttcttt agcaagacca acagatattg atatagcaaa gaatattgct aagtttccaa
    51841 aaagtactcc tgttatagct tttgtaaaac ccaatattcc ttgaataaaa ttatttgcta
    51901 aaaaaggcat atcactaact atattaggat ttgaaaatgc tgaacttatt cctaaaaata
    51961 tacctgctgc agatattaat gctattggca ataaaaatga ttttcctaac tgtaaaaaga
    52021 accctttcat tttttgtttc atttctatcc ctccattttt aaatttttaa aacgttttct
    52081 atgtcataat tatatatttt tattttgttt ttgaaacttt ataatttgtt ttaaaatatt
    52141 tttacataaa tagtaatttt ttcaaaactg taaatttttt caaacaaaga catagtaacg
    52201 ttgtaacata tataaattta aaaagagctt agaaaataaa attattgaat ataaaatgga
    52261 aaaatataaa ttacacaaag agatatgagt acgtactaac aaatacgaaa atgtataaga
    52321 aattataaaa aagtacatca agaaaattta agatgtactt tttagattgt atttagactt
    52381 ataattatat agttaaatga ataacaaaga attaatctta gtactattaa atacaaaaat
    52441 ttttatattt tttctatcta aattcttctt tgtttaatat atattgaaga gtatttattg
    52501 cttctaaata tttttttctg tcttcttcag ttaatttaga aagtttttct cctaaaccat
    52561 cacttaattt atccataaaa tcagttgcta tcttatctcc ctcttcagta atgtcaatta
    52621 gatattttct tctatcaact aaatctcttt tccttgttac atacttctta tcagttaaat
    52681 catcaaccat actagttaaa ctgccttttt ctatatttaa cttagagcat aaatcagtca
    52741 tacttattac tccataattt tttatgaaaa ctaatgctct taattgtgtt ttatttacat
    52801 tataatctgg tgcaaatttt tttaggtctt ctaagtacag gctagaataa atcttaggaa
    52861 atgtttttac taaaaaactt atggtatctt ttactatata gttcatataa tcacctcggt
    52921 tgttttaaaa tttattaaat tatctaaata actttatttc agtttacatt aaaaatattg
    52981 caagtacaat agtttttgct gaatgtttaa atttaattag taatcttatc atttttttat
    53041 ttttatttat attattccta ttaaatatta tttatatagc tattttatta aattcatggt
    53101 atagtaagta aagaagaact cttcacagcg gtggagagaa gacgctagcc cattaaaatg
    53161 gctatcttat ttataaataa gatagccgtt tttaattata ttttatctgt acaattctat
    53221 actcttaagt ctacatctac ttttcctaag tcttccttat aatcttttat agcaggttct
    53281 acaacttcat ttacaaattc tacaacttgt tctggagctc tacctataaa gttttttggg
    53341 tccattatag atagtatttc ttccttgctc attttaaatg aatcatcatt cattataagt
    53401 tcaattaagt tattatcctt gccttcatgc tttactctat aggcagcttt catagaatat
    53461 tctcttatta tttcatgtaa ttcctgtcta tctccacctc tttttacagc ttccataagt
    53521 attgtttctg tagccataaa aggaagttct tcatttacac gtctatttat catattttca
    53581 tatactacta aaccatcagt tacatttatc cctatttcaa gtatagcatc tgctgccata
    53641 aatgcttgag gtattgccaa tctcttattg gctgagtcat ctaatgttct ctctaaccat
    53701 tgagtggctt gtaccattgc agggcttata gattcagaga ttatatactt agataaagat
    53761 gctattctct cacttctcat tggattcctc ttataagcca ttgctgatga acctatttga
    53821 tttttttcaa atggctcttc taattctttt aaactttgta aaagtcttat atcattagtc
    53881 atcttatgca tactttgagc tatccctgat agtatgctta ttacttgata atctaacttt
    53941 ctagtatatg tctgtccact tactgcatag gaagaatggt atcccatctt tttacaaatt
    54001 ttcttatcta actcttttac tttttcgtgg tctccttcaa ataagcttaa aaaacttgct
    54061 tgagtaccag tagtaccttt aactcctctt aacttcatgt tgtctattct atagtttaaa
    54121 tcttccaaat caagaattaa atcttgagcc cataaacaag ctctttttcc tactgttgta
    54181 agttgagcag cttggaagtg tgtaaatcca agagctggca tatctttgta cttaagtgca
    54241 aattctttta aattgtttat taaatttaca agctttactc ttagaagttt taaagcttca
    54301 ttcatttgta taacatctgt attatctcca acatatgcag atgttgctcc taaatgtatt
    54361 attcctttgg ctttttcaca ttgtaaacca taagctttta cgtgagacat tacatcatgt
    54421 cttacttctt tttctatttt tctagcttca tcaaaattta tattatctat attagctctt
    54481 agctcatcta attgttcttc agttatattg attcctaact catgttcacc ttctgcaagt
    54541 gcaacccata accttctcca tgtagagaat ttaaattgtg gagaaaatat ataactcatg
    54601 tccttgctac aatatctgtc tgtaagtggg tttgagtaag tagtatattt attatctatc
    54661 attatattag caccttcctt aaatcttttg tcgtgtgatt actgtgtaca cattagtaca
    54721 taaataatta tatataattt tacgaactat aacaagactt ttttattttt tgttcacatt
    54781 tatatatagt attttttatt tattactatt ttttatttat atccaaatta tctttttcaa
    54841 ttaagtaatc cactatttca ctatcaacaa aatttaatac atacttatat ctaattgcat
    54901 agtatttatc actccatcca agctgatttt ttacatcact ttcatctgcc ccaagcctta
    54961 aacttattgc tgcaaatgaa tgtctaaaat ctcttgctga atattgagaa agtccagcta
    55021 aatctaatgc ttttttgact ataagtctga catttctatc tgttatagaa ttactctttt
    55081 gagttgtaaa aataaaatca tcactaggta ttattacctc tggaagccca gaataattcc
    55141 tgtaatcttc taaaagttta aaaaagtatg gatgtaattt aactactctt tcttttcttc
    55201 ccttacctaa ccttacatat gagttatcgt tttcatctac cataaggtct ttccacttta
    55261 agctaactaa ttcatttaat aaacacccag tagtaactag acagactatt attactctat
    55321 ctcttatatt taattttggt aggataccta taagtttatt tatttcttgt atacttagaa
    55381 catcatcttt tgtttttatt cttgttactg tgggtttttc tacatatcta aatggattta
    55441 tatctatgta tccttctttt tttaagaagt tataaaaact atgtaaataa ctatatatct
    55501 tttcacaagt tgatttcgca tatttagtct gtatatagtc tacaaaattt ttacaatctt
    55561 ctttcgtagc ataaatcaat tctttccctt ttaaaaattc tttaaacagt ataacctttg
    55621 ataaataatc gtgtttcgtg tttggattta attttgtaga aaaaacatta aaagtttcat
    55681 actcataatc atctagtatt tttgtactat acataataac tcctttcctt ttcataaaat
    55741 aaaatagcta tacttttact ataccatata tttccccctc aaatataccc cttattcaat
    55801 agataacttt taagtttatt ttgttatatt gtgaaggttt tatgaaaata tcaatcttcc
    55861 attgtgcact acacagtact atggtattat aagttagtaa aaggtggcat ttttttatta
    55921 atctatgaat aaaatatgct actataaatt tgtattggag gtattttgat ttggaaacat
    55981 ttagtttgct tgaaattttt aaggcagtca ttttgggcat agttcaggga ataacagaat
    56041 ggctaccagt aagtagtaca gggcatatga tattagttga tgagtttata aaattgaatt
    56101 tttcaaatac atttataagt acatttttag ttgtaattca atttggttca atattagctg
    56161 tacttgttat attttttaga aaattaaatc cttttgatag cgcaaagaat attaagcaga
    56221 aaaaagaaac agtccgttta tggcttaagg ttataattgc agttatacca tctggagtta
    56281 ttggaatact gtttgaagat gatatagata gattattctt taattcaaca gtagttgcta
    56341 ttgctcttat tgtatatggt ataataatga tagggcttga aaagagaaat aaaaaaccta
    56401 agtataaaga tttttctcaa gttacatata aattagctct ttgtatagga ttattccagt
    56461 gtctagctct tataccaggt acatctaggt ctggttctac tataataggt gcagtattac
    56521 ttggtacttc tagatatgta gcagcagagt tttcattctt cttagccatc cctacaatgc
    56581 ttggagctag tgcattaaaa cttctaaaag ctggatttgg atttactgga tttgaatggc
    56641 ttattttagg tgttggttct gtagtagcat ttgttgtttc aattgtagtt attaaatttt
    56701 ttatggacta tatcaaaaaa catgacttca aagtatttgg ttattataga atagtcttgg
    56761 gtatagtggt tcttgcttat ttctttctat tataatgtat tctaatttaa taacaaatga
    56821 taaggagaaa agttatgagt gttaaaaaag aaatatttga ttggattaag tcaatagcta
    56881 tggctattgt acttgcattt gtaattctac aatttataag accttctatt gtaagtggag
    56941 aatcaatgta tcctacttta gatgataaag attatctgat tttaaatagg atatcataca
    57001 aggttggtaa acctgaaaaa ggcgatattg tagtttttaa aaccaattta gttgatggag
    57061 aaacaggaaa gaaaaaagac ttaataaaaa gagttatagc tactgaaggt gacagaataa
    57121 aaatatcaaa ttctaaagtg tatgtaaatg gaaaattatt aaatgaacca tatatacaca
    57181 ataactatac ttctggagat atagatactg ttgttccaaa aggtaaacta tttgcaatgg
    57241 gagataatag agaaaatagt aatgatagta gattccctga tgtaggtatg gttgatgaag
    57301 atgaagttct tggtaaggtt atggtgagac tattacctct tgataatatt gggaaagtag
    57361 actaaaaaat aagagactga attggtaaag tttattttac aaatctcagt ctctttttat
    57421 tttcttaaat tacaaatatt tttttactaa atatcttttg attctgtaat aagtcttatt
    57481 atatcacttg cttcttcatt aaaagttgtt actatactta ggtagtctgt tgctctactc
    57541 atagctaaat aaaccttatt taaatctcct acaaagtcat ttacttgata atcttgttca
    57601 atatcattta tagtttgatt gtataacatc tctaattcac atactattac cgctttatac
    57661 tctaaattct ttattgcata tatatttgct attgtagttc ctaccttctt acttatattt
    57721 gtcaaattat cttctgcaca tatataagga atttttgcct cttccaatgc tttttttaac
    57781 atatattgaa agtaaatggt ttttccactt tttaatttct tcttattgta aggataaacg
    57841 atagcaactt cagaataatc aagacctttt ttacttatta aatactcaat ttcccagatt
    57901 acagaactta tttggtcatc aaggtcactt acttttatta tatcaacaga cttatttcca
    57961 cctctaagtg ctttagtata aaagaaagta ctaaattctg tatttggtct taattcgttg
    58021 atatatgagt tagaattatt tgaaaattta tttgtaaaat ctacaatctc tttagcttgt
    58081 ctataatttt tacttaagat aatcttatca tcaaattcta taccattata taatttttta
    58141 aatatattca atctattaga gatattcaag gagttacaag aacacacatt aaatatgaat
    58201 tttgttttat ataaaaactc ccttataaaa tctatttcat actctaaaaa gctttctgct
    58261 tcatcaataa aaatagcctt gaacatattt ttatttttta taatgttctg tgcttgtttt
    58321 acaagattat taaaggtctt ttcgtaatca ttttttaaca tgttataatc tacaattaaa
    58381 ttgaattttt tagctagttt aaatataaaa gaactaaatg tgtgaacttc taaattgtta
    58441 ttatctttgt ataataactc tatcctctct cgaagctcat tgcacaattg ttttgtatgt
    58501 gtaaatatga ctaatttatg atgtggatat acttttgcta attttatagc cctagaaagc
    58561 ataattgctg ttttgcctat accagaacca ccttcaataa gtgtattccc atatttaata
    58621 gaaacaacat ctttaatttg aacgtcttcc atcatagttg ctgtatactt atagtcatca
    58681 ttgtagaatg aaattttttt aaatttttca tttagatgaa gtttatcatt tattatatag
    58741 tattctgaac atatgtctag taaaaataaa tttaagttta tttcatcatt ctcatcatta
    58801 agatattcct ccagcgaacc cttattcatt atatcttgaa gcttattttt atctattata
    58861 ttattattaa caaattcttc aaattcatat gtttcctcaa cttcaacgta tggcataatg
    58921 taaacgtagt tataacttat atttctattc ttttgtccca tttttagttt caaaagttca
    58981 tattcctcat tcataacttc aagtaactct tcttctaaaa taaaaaatat atcttctgtt
    59041 gtatccataa attttataaa taaaattctt ccatctttaa catacaacaa atcggtattt
    59101 attcctttaa aaggagtaac ttttggcact actttaacgt cctcattgat attttcgtaa
    59161 aatattttct cgaactcctt caattttagg gcgtctaact gagaatttat atttatgtat
    59221 tggctcactt acattacctc cttctgttat acttaattat accattttga actatatttt
    59281 tatatatact ttttataggc aaatttagta aataaattca aaataaatac aaataaatac
    59341 aaaactgcta aaaaaatttt gcaataagta ta
//
LOCUS       DBGJFU010000009        58903 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.009, whole genome shotgun sequence.
ACCESSION   DBGJFU010000009 DBGJFU010000000
VERSION     DBGJFU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.009"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            209..1285
                     /locus_tag="ACOIAS_000548"
     CDS             209..1285
                     /locus_tag="ACOIAS_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGZ2700279.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1402..2244)
                     /locus_tag="ACOIAS_000549"
     CDS             complement(1402..2244)
                     /locus_tag="ACOIAS_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGZ2700280.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            2428..3000
                     /locus_tag="ACOIAS_000550"
     CDS             2428..3000
                     /locus_tag="ACOIAS_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2700281.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3163..3435)
                     /locus_tag="ACOIAS_000551"
     CDS             complement(3163..3435)
                     /locus_tag="ACOIAS_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700282.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3562..4434)
                     /locus_tag="ACOIAS_000552"
     CDS             complement(3562..4434)
                     /locus_tag="ACOIAS_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2700283.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4721..7828)
                     /gene="ileS"
                     /locus_tag="ACOIAS_000553"
     CDS             complement(4721..7828)
                     /gene="ileS"
                     /locus_tag="ACOIAS_000553"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGZ2700284.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7921..8153)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8328..8855)
                     /locus_tag="ACOIAS_000554"
     CDS             complement(8328..8855)
                     /locus_tag="ACOIAS_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HGZ2700285.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8890..9675)
                     /locus_tag="ACOIAS_000555"
     CDS             complement(8890..9675)
                     /locus_tag="ACOIAS_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HGZ2700286.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9685..9942)
                     /locus_tag="ACOIAS_000556"
     CDS             complement(9685..9942)
                     /locus_tag="ACOIAS_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HGZ2700287.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9961..10419)
                     /locus_tag="ACOIAS_000557"
     CDS             complement(9961..10419)
                     /locus_tag="ACOIAS_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HGZ2700288.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10459..11163)
                     /locus_tag="ACOIAS_000558"
     CDS             complement(10459..11163)
                     /locus_tag="ACOIAS_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HGZ2700289.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11173..11808)
                     /locus_tag="ACOIAS_000559"
     CDS             complement(11173..11808)
                     /locus_tag="ACOIAS_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HGZ2700290.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12189..13229
                     /locus_tag="ACOIAS_000560"
     CDS             12189..13229
                     /locus_tag="ACOIAS_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGZ2700291.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13692..15050)
                     /locus_tag="ACOIAS_000561"
     CDS             complement(13692..15050)
                     /locus_tag="ACOIAS_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HGZ2700292.1"
                     /translation="MVNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREK
                     IYNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDK
                     AAEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMD
                     AVKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGV
                     SGPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSL
                     APTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIP
                     VSEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGM
                     INNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSF
                     KN"
     gene            complement(15073..15342)
                     /locus_tag="ACOIAS_000562"
     CDS             complement(15073..15342)
                     /locus_tag="ACOIAS_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGZ2700293.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15632..17587)
                     /locus_tag="ACOIAS_000563"
     CDS             complement(15632..17587)
                     /locus_tag="ACOIAS_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700294.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17770..19245)
                     /gene="spoIVA"
                     /locus_tag="ACOIAS_000564"
     CDS             complement(17770..19245)
                     /gene="spoIVA"
                     /locus_tag="ACOIAS_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HGZ2700295.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19417..20712)
                     /locus_tag="ACOIAS_000565"
     CDS             complement(19417..20712)
                     /locus_tag="ACOIAS_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGZ2700296.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(20896..22341)
                     /locus_tag="ACOIAS_000566"
     CDS             complement(20896..22341)
                     /locus_tag="ACOIAS_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2700297.1"
                     /translation="MSLNKFEIGGITVKKKIGFWDLVFMNISALFGIRWIAKSTASSF
                     GLGLGSIPVWLLFAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLN
                     WTSKIFWYSSFLTFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGK
                     FFTNTGALGSTIPAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAII
                     FALSGAETTANFITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAAST
                     GILDALAKVAQDLGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQ
                     LTVTNKYNIPSNAVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFT
                     SYIRLRKTRPNEERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYE
                     IEMIGGGLFVIITGLMLWKNYEKKISNNSGR"
     gene            complement(22357..23496)
                     /locus_tag="ACOIAS_000567"
     CDS             complement(22357..23496)
                     /locus_tag="ACOIAS_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGZ2700298.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVEGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23815..24465
                     /locus_tag="ACOIAS_000568"
     CDS             23815..24465
                     /locus_tag="ACOIAS_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2700299.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(24622..25641)
                     /locus_tag="ACOIAS_000569"
     CDS             complement(24622..25641)
                     /locus_tag="ACOIAS_000569"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGZ2700300.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25672..26286)
                     /gene="plsY"
                     /locus_tag="ACOIAS_000570"
     CDS             complement(25672..26286)
                     /gene="plsY"
                     /locus_tag="ACOIAS_000570"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGZ2700301.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26321..27646)
                     /gene="der"
                     /locus_tag="ACOIAS_000571"
     CDS             complement(26321..27646)
                     /gene="der"
                     /locus_tag="ACOIAS_000571"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGZ2700302.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27674..29002)
                     /locus_tag="ACOIAS_000572"
     CDS             complement(27674..29002)
                     /locus_tag="ACOIAS_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HGZ2700303.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADTHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29240..29440)
                     /locus_tag="ACOIAS_000573"
     CDS             complement(29240..29440)
                     /locus_tag="ACOIAS_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700304.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29442..30374)
                     /locus_tag="ACOIAS_000574"
     CDS             complement(29442..30374)
                     /locus_tag="ACOIAS_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HGZ2700305.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30419..31351)
                     /locus_tag="ACOIAS_000575"
     CDS             complement(30419..31351)
                     /locus_tag="ACOIAS_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HGZ2700306.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31406..32695)
                     /locus_tag="ACOIAS_000576"
     CDS             complement(31406..32695)
                     /locus_tag="ACOIAS_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2700307.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32724..33416)
                     /locus_tag="ACOIAS_000577"
     CDS             complement(32724..33416)
                     /locus_tag="ACOIAS_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700308.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33431..34192)
                     /gene="pgeF"
                     /locus_tag="ACOIAS_000578"
     CDS             complement(33431..34192)
                     /gene="pgeF"
                     /locus_tag="ACOIAS_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HGZ2700309.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34189..34650)
                     /gene="nrdR"
                     /locus_tag="ACOIAS_000579"
     CDS             complement(34189..34650)
                     /gene="nrdR"
                     /locus_tag="ACOIAS_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGZ2700310.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34672..34935)
                     /locus_tag="ACOIAS_000580"
     CDS             complement(34672..34935)
                     /locus_tag="ACOIAS_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HGZ2700311.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35039..35812)
                     /gene="sigG"
                     /locus_tag="ACOIAS_000581"
     CDS             complement(35039..35812)
                     /gene="sigG"
                     /locus_tag="ACOIAS_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HGZ2700312.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35904..36626)
                     /gene="sigE"
                     /locus_tag="ACOIAS_000582"
     CDS             complement(35904..36626)
                     /gene="sigE"
                     /locus_tag="ACOIAS_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HGZ2700313.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36651..37496)
                     /locus_tag="ACOIAS_000583"
     CDS             complement(36651..37496)
                     /locus_tag="ACOIAS_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HGZ2700314.1"
                     /translation="MVYIEYYVIENLVINYIIISCTSILTKRYNPKKNKLLGASLGGA
                     YSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLVNIFISGSTY
                     FIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTININLLEKSFCC
                     KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQEIIGSLDDK
                     TSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDREYGAIMNPN
                     ILPGF"
     gene            complement(37813..39087)
                     /locus_tag="ACOIAS_000584"
     CDS             complement(37813..39087)
                     /locus_tag="ACOIAS_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HGZ2700315.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39371..40528)
                     /gene="ftsZ"
                     /locus_tag="ACOIAS_000585"
     CDS             complement(39371..40528)
                     /gene="ftsZ"
                     /locus_tag="ACOIAS_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGZ2700316.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40718..41071)
                     /locus_tag="ACOIAS_000586"
     CDS             complement(40718..41071)
                     /locus_tag="ACOIAS_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HGZ2700317.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41170..41844)
                     /locus_tag="ACOIAS_000587"
     CDS             complement(41170..41844)
                     /locus_tag="ACOIAS_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HGZ2700318.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(41849..42547)
                     /locus_tag="ACOIAS_000588"
     CDS             complement(41849..42547)
                     /locus_tag="ACOIAS_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HGZ2700319.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIEIYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(42570..43310)
                     /locus_tag="ACOIAS_000589"
     CDS             complement(42570..43310)
                     /locus_tag="ACOIAS_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HGZ2700320.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(43580..44806)
                     /gene="murG"
                     /locus_tag="ACOIAS_000590"
     CDS             complement(43580..44806)
                     /gene="murG"
                     /locus_tag="ACOIAS_000590"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGZ2700321.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(44824..45954)
                     /gene="spoVE"
                     /locus_tag="ACOIAS_000591"
     CDS             complement(44824..45954)
                     /gene="spoVE"
                     /locus_tag="ACOIAS_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HGZ2700322.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(46006..47361)
                     /gene="murD"
                     /locus_tag="ACOIAS_000592"
     CDS             complement(46006..47361)
                     /gene="murD"
                     /locus_tag="ACOIAS_000592"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGZ2700323.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASVIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(47380..48348)
                     /gene="mraY"
                     /locus_tag="ACOIAS_000593"
     CDS             complement(47380..48348)
                     /gene="mraY"
                     /locus_tag="ACOIAS_000593"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGZ2700324.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(48389..49762)
                     /gene="murF"
                     /locus_tag="ACOIAS_000594"
     CDS             complement(48389..49762)
                     /gene="murF"
                     /locus_tag="ACOIAS_000594"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGZ2700325.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(49929..51908)
                     /locus_tag="ACOIAS_000595"
     CDS             complement(49929..51908)
                     /locus_tag="ACOIAS_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HGZ2700326.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(51920..52267)
                     /locus_tag="ACOIAS_000596"
     CDS             complement(51920..52267)
                     /locus_tag="ACOIAS_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700327.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(52753..53688)
                     /gene="rsmH"
                     /locus_tag="ACOIAS_000597"
     CDS             complement(52753..53688)
                     /gene="rsmH"
                     /locus_tag="ACOIAS_000597"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGZ2700328.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(53718..54464)
                     /gene="lgt"
                     /locus_tag="ACOIAS_000598"
     CDS             complement(53718..54464)
                     /gene="lgt"
                     /locus_tag="ACOIAS_000598"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGZ2700329.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(54858..56144)
                     /locus_tag="ACOIAS_000599"
     CDS             complement(54858..56144)
                     /locus_tag="ACOIAS_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGZ2700330.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(56159..57424)
                     /locus_tag="ACOIAS_000600"
     CDS             complement(56159..57424)
                     /locus_tag="ACOIAS_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGZ2700331.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSSKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            57740..58837
                     /gene="ychF"
                     /locus_tag="ACOIAS_000601"
     CDS             57740..58837
                     /gene="ychF"
                     /locus_tag="ACOIAS_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGZ2700332.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        18459 a        10675 c         6339 g        23430 t
ORIGIN      
        1 aactttatag acttaattta ttacttttat agaattttca caataaaaag tcattttact
       61 tccgtattta atgcaaaagt agagattaaa cttaatataa aaaatatata acaaattttt
      121 tttatatttc tagaagaaaa gtatactctt ttttgtttaa taatgctaaa attgatttgt
      181 attgaatttt gattaggagg aaattattat gaaagctcaa agattaaatg ctgtacttga
      241 acaaatgaaa aaagatgata tttctcaaat gttagtaagt gaccctactt ctatatttta
      301 tcttacagga gtactaatcc atccaggcga aagactatta gcattatact taaatttaaa
      361 tggcaataat aaattattta taaatgaatt attcccagtt tcagaagatt taggtgttga
      421 aatggtttgg ttcaatgata ctcaaaatcc agtagaaata ataactgaac atatagacaa
      481 aaatgctact atgggtgttg ataaaaactg gccagctaga ttcttattaa acttaataga
      541 attaggtgga ggaagtaagt ttgtaaattc atcttatata atagatactt taagaatgtg
      601 taaagatgaa gaagaaaaag agttaatgag aatagcttca aaattaaatg acaaggctat
      661 ggaacaactt aaagctactg tttctggtga acttacagaa aaacaacttg ttggtaaatt
      721 atctaaaata tatgaagacc taggtactga tggattttca tttgacccta ttataggttt
      781 tggacctaat ggagctaatc cacatggtga acctggaaat gcacttgtga aaccaggaga
      841 tgcaataatc ttagatatag gttgtataaa agataactat tgtgctgata tgacaagaac
      901 tgtattctat aaagaaatac ctgaaaaagg tagagaaata tttgaaatag ttcttgaagc
      961 taataagaga gctgaagcta ttgtaaaacc aggtgttaga ttctgtgata tagatgctgc
     1021 tgctcgtgac tacataactg aaaagggata tggacagtac ttcactcata gaactggtca
     1081 ctctatagga cttgaagttc atgataaagg agatgtatct tctataaata cagatactgt
     1141 acaaccagga atgatattct ctatagagcc aggtatatat ttaccaggtg agttcggagt
     1201 tagaatagaa gatttagtac ttgttactga agatggttgt gagatattaa acaaacatga
     1261 caaagaaata tgtgtagttg gttaattcta aaatcaattt gataaaatta aaaagccata
     1321 taaaaatgtt ttaaacattt ttatatggct ttaatttctc aatctatgat ttatttcaat
     1381 ccattattta tattgcagta tttatattgc agttattaag attgcaccct ttgaagtata
     1441 tgtccccatc actgaaccaa cttcacagat tacaacatct ttgtatttta catccttttc
     1501 cataacttcc cttacctttt gagccttttc tgggcaatta gcatgtccta taaataaaac
     1561 cttttcttct ttatctttaa tttttgactc tactaaagta atcagctttt tcaaactgtt
     1621 tccttcacct cttgccttat ctataggagt tacaacgcca tctgcaactt gtattatcgc
     1681 cttaaaattt aaggtattta taatttttcc tgctattgga tttattctac cacccttaat
     1741 agcattttct aaagtttcaa gtgttccaaa aaatggagga ttatctttat atttatttat
     1801 tattgaaaca atctcttcaa tttcttttcc ttcttttatt aatattgctg cttttaacac
     1861 taattgtcct attccaattg acccactaag tgaatctata acctcaactt tcttgttatt
     1921 tccttccttt tcaaacatat cttttgctaa aagtgcacta ttataagtcc cagataatcc
     1981 tgaacttata ttaatcacta atacactttc ttcctgacaa tggtatgctt ctaaaaattt
     2041 atcaggagaa ggacatgatg tttttggtag tactttactc tcactcattt ttctatagaa
     2101 agtttcatta tctatttcct ttccagatat ataactttct tctccaaaag atacatttaa
     2161 accaacaact tctatgttgt atttttctat tatatcatca ggtaagtcac atgcactgtc
     2221 cactatcaat tttattttat ccatattaac ctcactataa gatattattt tatttattca
     2281 gtcggtcgta cgaccgattt aattattatt ttatatattg aatgaaaaaa agtcaataga
     2341 tatagattct cgctttattg acaataaata tccataagct tataatgtta ttaatttgtt
     2401 ataaatatac atggaaaggt aggttttatg aaaacaaata atatgaaaca ggctatttta
     2461 tcatctgcaa ccaatttaat agccaaaaat ggagttcaaa atactagcct tgctgatatt
     2521 gcaaaagatg taaatataag taaaggtact ttgtattatc actacgcatc taaagatgat
     2581 attatatatg atattgcgga tactcattta gaagttataa ctagtgctat actaaactgt
     2641 gtaaaaaatg tcaaatcaaa aaattctcaa atagaaatgg ttaacttaat cttagagaaa
     2701 atatctacta tagaaagtcg tggtagggta catatgtatc ttatttgtga ggcaattact
     2761 tcaaataatg atttaaagga aagaataaga ttaaaatata ttgaatggag aactactctt
     2821 aaatctgaaa ttgttgaatc actagaaaaa tataatagta gcgaaaatga acaggatgct
     2881 gaatcatttt cttttttact catgtcaata gttgatggtt tagttgtcca aagtttatta
     2941 aaaacagaaa aaatacctta tgaaaatata gcttcttttt tagttaatca ttggatttaa
     3001 acttaatact tagataatta ataaaagtga actatttaag ttatctactt taacagttca
     3061 ctttcttatt tattctcaat aatttttact taaaacattc aatttttatt taaaccttca
     3121 tggaatcaca aaataaaaat ctcttataaa tttaaaatgt ttttatttat gttcatgtgt
     3181 atgcccatct gaatgttcat gaccatgtgt atgtgtatgc tcatggctat gctcatgagt
     3241 acctgaaaaa ccatcatcat gattatggtc gtgacctaca ccatttacat gctcatgttc
     3301 atgcccatga ctgtgagaat gttgatgtga atgttcatgg ttatctcctc cacagcatcc
     3361 ttctcctcca gtcccacaac acccacttgt atgtggatta tgatgatttc ttactacaaa
     3421 tctctctctt aacataattc ttatacctcc tagtatatta ttgagtactt catgtacccc
     3481 attaactatg gtatacatct aactaggtat aatctcaata catatgtcat tttttaacat
     3541 atataattaa catatataat cttaaagaaa tttatcctta aaacctccac cttttacttc
     3601 ttcaacgtta ttgacagaaa taaaagcttc actatccata tcataaatta aatctttaaa
     3661 tttacctatt tcatttgcag atattatgca ataaatcatc tttttcttat tttgtgtata
     3721 tgcaccttct gcctccaaaa aagtgactcc tctacccatt ttattcatta tcactttgga
     3781 tatttcatca tatttatttg atattaaaag tatagatttt tttctatcaa agcaatcttt
     3841 tcctatatcc atagtaaaag aaattatata cattgtaatt aaagtgtaca tcaccaattt
     3901 agctccaaat aaaaaagctc ctgcacatac tacaaaaaaa ttaataaata aaaatgtatt
     3961 tttcataggt atatcatact ttcttttcat tatggctgct attatatcaa gtcctccagc
     4021 agtagctctt gctttaaaag ttaaccccat acctatacca gttaatgcac caccataaat
     4081 acactgtagc atagtatcat ctattgggaa atatttagca atatcttgtg ttagccccag
     4141 tgctagagaa aatagtaaca tattgacaaa acttattagc aaaaatttat tatcaaaata
     4201 ctttctagct ataataaata ttggtatgtt catcaagaaa cttgatattc cctgatttat
     4261 accaaataat ttgtataata taacacaaat tccagctact cctccactta ataacttggc
     4321 tggtgctaga tataaattta ttcctaaggt tgatagaatc atacccaata taatcagaca
     4381 atattgtaaa aatatattat tatcaatact tagcttttta ctttcaccat acatcaaaac
     4441 gcaccccttt gcataataaa taaaccaccc tgcataacac acgataaatt tacaaataaa
     4501 tttatcagaa gtagtttcct aaaaatatta ttcatcctca cttaaacgtt tattcattaa
     4561 tattctatca catgacataa ataatttata tgatttatac agatttattt attgtaaatt
     4621 ttggaatatt aaaacatttt attgaataga taattatatt acataaaaaa tccctataga
     4681 aatctataaa ctaatttcta tagggatttt gattaactaa ctattttttt ataactttta
     4741 tacctgtcat atggtcattt aagttttgtt cctctaaaga tttatcatct actctgttta
     4801 tttctaaagc taaagtttca gacttgatat attcattaaa atggtcaaca gctttagcaa
     4861 tttcatcatc accacagtat tctattacta tattatctaa tacttcaaag ttattagatt
     4921 ttcttaattg ctgtacttta gatataaatt ctctagcata accttcatta atcaactctt
     4981 cagttagctt agtatctagt ataacaaaaa gattgttttc aacagataca ttaaatcctt
     5041 ccttagcagt tatacctatt aatactaaat ccttattaaa ttcaaaagct tcaccatcta
     5101 aatcaactgt taaagtctca cctttttcaa gtttattagc agtttcatga gcatttaatt
     5161 tacttaatgc tccaacgaat aaattcatct tagaacctaa taatggccct gcttccttaa
     5221 agtttggttt taaactaaaa ttcatatact catctaaatc tttagcaaat attacctctt
     5281 taacattaag ctcttctttt attaaatcaa ctacatcact tattgtatct tcaaacttac
     5341 catctactaa cactttttgt aatggttgac gaacttttat tctttctact tctcttgaag
     5401 ctctacctaa agtaactaag tttttagcta aatccatttt ttcttctaat ttagtatcta
     5461 ctaactcttc attacattta ggatattcag ctaaatgaac agatacttct cctgttaagt
     5521 ttctatacat ttcttctgac atatatggtg caaatggagc tatagctcta caaagaccat
     5581 gtaacaattc ataagttgta ttgtacactg cttttttatc ttctgttaat tcagttgccc
     5641 aaaatcttct tcttgaacgt cttatatacc agttagataa atcatcattt ataaaatctt
     5701 gtatcattct taaagtttta tttaactcaa atatagctaa attttcttct acatctttca
     5761 ttaaattatt gaacttagat aatatccatc tatcaagttc tggtctatct ttatattcta
     5821 caaaaaattc agttggattt atatcatctg tgtttgcata taaagtaaag aagttatata
     5881 cattcttcat agtacctata aatttacttt ggatttcttt taatccatcc atatcaaatt
     5941 tagttggagt ccatggtgga gaaacatata gtaaatacca tcttaatgca tctgctccat
     6001 attggtcaaa taattccatt ggatttactg tatttcctct tgatttactc attttcttac
     6061 catctttatc aagtacaagg tctggaacta aaacactctt atatggtgct tttcctaata
     6121 caaatgttga tactgcaagt aatgagtaga accaacctct agtctggtct ataccttctg
     6181 atatatagtc agctgggaac aattggtcaa agttttcctt attttcaaat ggatagtggt
     6241 gttgagcaaa tggcatagcc ccactatcaa accaacagtc tataacctct gttactcttg
     6301 tcattggttt tccacatttt tcacatttta agtgtatatc atctacatat ggtctatgaa
     6361 gttcaactgt ttctggattt acatcttcta tagctttttc agctaattca gctcttgaac
     6421 caactgaatc tgtatgacca cattcacatc tccatatagg aagtggagtt ccccaatatc
     6481 tagttcttga tattgcccag tcatttagat tttctaacca gtttccaaat ctcttctctc
     6541 ctacaaaact tgggaaccat tttacaccat tattattttc tattaattta tctttaagtt
     6601 tagtcatctc tatataccag cttggttttg catagtatac taatggagtt tgacatctcc
     6661 agcagtgtgg ataattatga actacttttt ctttgctata taatttattt tcagaagcta
     6721 gccattttat tatttcaaca tctagcccat cttccattac aaatctacct tcccaaggag
     6781 tagatgtaaa tttaccagtt tcatctactg gttgaataac tggtaaatca tatcttttac
     6841 ctgtgttgta gtcatcttca ccaaatgctg gtgctgtatg aactatacca gttccatctt
     6901 cagtagttac ataatctcct actgtaataa aaaatgcttt tttgtcaact tctacaaaag
     6961 gcattaattg ttcatattct aaaccttcta ggtctttacc tttcatttct tctaaaactt
     7021 cataatcttc acctagtact ttatcagcta aaggtttaga tacataatat acctcatcac
     7081 cttttttaac ttttaaataa tctacatcag catttactgt taatgaaaca tttgatggta
     7141 aagtccatgg agttgttgtc catgctaaga agtattcatc agcatctttt ttcttaaatt
     7201 tagctataac tgtattagtt tttacttctt tatatccttg tgcaacctca tgactagcaa
     7261 gaccagttcc acatcttggg caataaggaa gtattttatg cccttcatat atatatcctt
     7321 ctttgttgaa tttgtttagt atccaccata cagactctat atagttatta tctaaagtta
     7381 tatatggatt atctaaatca acttcataag ccattctctc agtcattatt ctccattgtt
     7441 tttcaaatga gaaaactgac tctctacatt tttggttaaa ttcagctatt ccatattctt
     7501 ctatttgttg cttactagta agtcctaact ctttctctac ttgtatttca actggtaatc
     7561 catgtgtatc ccaaccagct tttctcttta cttgataacc agacattgtt ttatatctac
     7621 aaactgaatc tttaagcgtt ctagataaaa catggtgtac tccaggattt ccattagctg
     7681 ttggaggtcc ttcatagaat acgaaacttg ggtcatcttt ccctttttct aaagtttttt
     7741 caagtatatt tatgtctttc caataatcaa aaacttgtgc ttctgcttgt tttacagaac
     7801 tgtctaccaa tggcttaaat ttcgccatgt aaatcaccct ttcctactaa atattataat
     7861 tccaaaattt tttattcttt cacataaatt taaatactaa aaaactcgcc ccctttaaac
     7921 caaagggacg agttataaat cgcgttacca ccctaattct atatgtaaac taaatactag
     7981 ctacatataa cactctaata gtataacggt actaaccggc acagcttagt agaatttaat
     8041 cgttcagcct gcagctcaag agtgattttt atctttataa attcattggt tctcagcata
     8101 cccaattctc tgtaaaatta tctaaagata aatgtctcta tcaacgcttt taaaaattta
     8161 tctgtgttta tatgaaattc tataacaaat aatttgaata gtcaatatat ttttgaaaaa
     8221 tataataatt tctatttttc ttacattttt atcctatttt taatctttgt tatagatttc
     8281 tcaaactcac acaattattc tacataaaaa ataaacaaat tacaagctta ttctaaagtt
     8341 gtagcagctt catcattact ataattatac actgttgttc cttcaaatgc gtttacacat
     8401 ttttcatcta catcataaaa aatctcatca acacttctta tttcatcttc taacagtgat
     8461 ttaaatttat ttctaaatat cttaaattct ttgactaaag catcatactc ttttcttatc
     8521 tctataacac ggttattagc ttgttctata atttgtctag cttttaaatc agcttcttca
     8581 acaattatct tagccttttt attagccgcg ctacaagtgt cttctgctgc actttgagca
     8641 gttattaaag tagcttttag tgtttcttca atattttcat acttgtttac ttggtcttga
     8701 tataacccta atttttcttt aagagctgta ttttccctac atagagattc ataatcttcc
     8761 ttgatgatat ctaaaaattc atcaacctct tcatctctat accctcttaa acctttttta
     8821 aactctttat tttctatctc aattggagtt agcattacat ttcctcccag ctataattta
     8881 aattagtcct tatacaatta atcttgcttg aacttttatt tttccttttt tagtaatatc
     8941 tcctatttgt gttaccttag ctcttccttt gcctctaata gaaatcaaat catcattttt
     9001 tactatttta gaagttgaag ttatcttttc ataattgaca tggacttttt ctccatttat
     9061 gtattttgca ctatcttgtc tagatatatt gtaaatacca cttatcacac aatctaacct
     9121 atctgaagat actgtaaatg atacttcttt atattttgaa gaactattaa caatattttc
     9181 tctggaaatt tcctttacaa ttacattatt tcttgaaacc ttttctaagt ttacaattat
     9241 aaaatcgcaa atatcactac ttacaactac ttgacaaaag ctatcatgta taattatatc
     9301 tccaattttt tctcttttta tacctagacc cagtattgac cctaagtaat ctttatgaga
     9361 tatattttta aatttgaaat tcccttctat ttgtaaaaat ctaagagtat cttctatatc
     9421 ttcatattcc atgtaaaatg gataaataaa tacagtgctt ctttcagctt gttcataacc
     9481 tccatccaca ctgtatttta tttcatttgt agaatttaat attgctacag catttttcac
     9541 ttcatatgga ttaagaaact ccgtagactt aacatcatag ttttttatac aactattagc
     9601 cttgtctatc actttaaaca ttttattttt caagtctata tcctttatgt gatttgttag
     9661 ttttaattta tccatcctat caccttagaa tataaccaat actaattgtc ttaggaacat
     9721 caataatact attgcaatta aaggagaaaa atctataggt ccatttgtat atttatacat
     9781 tatagacctt attggccctt ctattggctc tgtaagattt tccaaaatat caaaaatttt
     9841 actttgtctt ccatttggaa accaagtcat caagcttttt ataactatca tccatgctat
     9901 tatatctaaa agataataaa gtgcaattct tattgttccc atatattaac cgccttttta
     9961 ctatttattt tgccaaggga agaatgcttt actttctaat tcttttttca tatttgcatc
    10021 tatatccaca ttattaggag ctagtataaa tattgattta gaaacctttt gtatacttcc
    10081 atctaagaca taaactgccc cattcataaa gttaaatata gatttacgaa cttcagaatt
    10141 agtcaatcct tccaaattca ctataactgc ccttctttgt tttagatggt ctgctatagt
    10201 tgcagcttca tcataaacct taggttcaac tattacaact ttcatttggc tagttgtatg
    10261 taaatttaca attttatttg cttttgcagt actaaagcca ctgttcattt cttcttcttg
    10321 aactatatcg tccatgccac tttcatattc atcttcaatg taatcttctt cttcatcaac
    10381 tatccaattc ttaaatttag atattattcc atttgacatt attaacttcc tccttaaaat
    10441 ttattcaaat ttgagtaatt attttttatt gtagtttctt tctccaaaga ttgatgttcc
    10501 aactctaaca atggtagccc cttcctctat agcaatttca taatcgtgac tcattcccat
    10561 tgacaaaaca tccatcccta cattagggat gtttagttct tttatttgtt ttgataaatc
    10621 tttaagacct ttaaatacac ctcttatctc atcttcatct tctataaaag gtgccattgt
    10681 cattagaccc tttattttta tatttttata tttttctgac acttctttga caaagtctac
    10741 aacttcttct ctatcaagtc catgcttaga ctcttcttta gatatattca cttgtactaa
    10801 gcaatctatt tctctgttta tttgttctgc acgtttttga atttcttcgc ataatgcaat
    10861 tctatctaaa gagtgtatca tacaaacttt atcgattatg tacttaactt tattggtttg
    10921 caaagttcct atcaggtgcc agtttaattt atcacctata atatcatact ttctagctaa
    10981 ttcttggggt ttattttcac caacagaagt gattccttcc ctcattgcct cattaactaa
    11041 gtctacatca acagtctttg tgactgccaa aagggtaatc tctttgctgt ctctatctac
    11101 tttttttgca gctttatcaa ttttgtttct tatctcagac aaattgtctt taatagaacc
    11161 cattattatc acctacttta tccttatatt tgtgtttata ctgttaggtc ttaaaattat
    11221 tttgtcatat agattaacac ttgtaactcc atttatgtca tttttataat aatccacaaa
    11281 tacagaagat tcattctcat aagttattcc ttctaatatg acaaatttta cactttgtga
    11341 ttgttcatca attacatata caccacgttt attgtctttt gtttttatag aagttttagg
    11401 tatttttaaa cattccatct gattatatat tatatcaaat tcttcgacac ttgtatcctg
    11461 atttgcaaca atttggttac ttatttttaa ttttaccatt attttattat tattttcata
    11521 tattttgtaa acttttgcat ctaatttttg ctcatgatat tcaattatta cattttgatt
    11581 tttttcaaag ttattagaat cttctttact gatgtatgta gatagataat tactctggtc
    11641 attggcatct ttttctatag ttccttttac atgaatcttt gcatcaactt tttcattttt
    11701 taacaccaac gtatctacat ttttgctaat tatcattact aatatattaa atactaaata
    11761 tataagaata ccaagtatca ctaatctggt atattttacc tttttcaaag actctccccc
    11821 tataaaatta ctttcattat aagtacttct tctaaaaaat gtttattcct tttttataag
    11881 ctaatatttt acgttctaat aattattttt tccatattaa atttattttt tatcaatctt
    11941 cctatacaag actgcattat ttatatataa ttgtattata aagtataagt ttaatttaca
    12001 ttttatacgt taagcttact ttatatgttt ttatagttat ttttattttt agatgcaata
    12061 taaaaccaat taagatttaa aataactttt gttatgttga catttattta ggtaatcatt
    12121 tatgaattag gataacgtta tcataacaaa agtttatgtc ctaaaaaaaa tacaaggagg
    12181 attttcttat gtcagaaatt ttattaggaa caggtttact tttattggtt ctatgttgtt
    12241 tcacaatttt tagttacaag gccccatatg gtatgaaggc tatgggagct ctagcaaacg
    12301 ctgcatgtgc tactttctta gtagaggcat tcaacctttc tttacttggt gatgttttag
    12361 gtatggaatt ttttagaagt gttggagctg ctaatggtag tcttggtgga gttgctgctg
    12421 ctatacttgt gcctttagcc ttaggtgtat ctccagttta tgcagtttta ataggattat
    12481 cttgtttaga tttttctata ttatctggat tcgttgctgg atactttgtt tctttcttaa
    12541 ttaagtttat agagaaaaaa gtgccacctg gagtggattt agtggttgta attcttctat
    12601 gtgcaccgct atctcgtctt atagcatcaa atgtagaccc attagttaat agtacactac
    12661 tacaaattgg acaagttttg atagaagcta ccaacattag ccctataata atgggaatta
    12721 tactaggagg tctaataaca gtagttggaa cgtctccact tagttcaatg gctcttacat
    12781 ctataattgg tcttactggt cttcctatgg caataggagc attaggtgct tttggttcag
    12841 cttttatgaa ctcaacacta tttgcaaaat tgaaattagg ttctaaaaaa gatattatat
    12901 ctgttgctat tgaaccatta acccaatcag acataatttc agctaatcca atacctatat
    12961 attctactaa tttctttgga ggagcctttt ctggtatcat tgtagccttg atgggtcttg
    13021 taaacatgac accaggtaca gctacacctg tggctggttt tgctataatg tttgcttata
    13081 atccagttca acaagttctt ataacagctg gtttatgtgc tttattcaat gtaattgcag
    13141 gatttttagg ttcaaaaata ttcagtacct ttaaagttct tactgctggt gaaatacgtg
    13201 gtactgatgt agaagaagaa attgcttaaa aaacaataag atttataaat ttatatctca
    13261 agtagtagag ttttatattg taggcaattt tattaattgc aaacgtttta ttttttattt
    13321 atgtttatga ttattatgtg tgttgaatat ttagataaat ttttatttat tatttttatt
    13381 tagatttttg tttagttttt tatttggttg atatattagg aataaaaatt agtatagttt
    13441 attactagtt agatagcata actttttaga tacataaaaa attttatatt aattagatat
    13501 tattaaaata aaagcgatac atgaattttt aaatgtccat gtatcgcttt atttttgcca
    13561 taatcttaac aactgatttt taaagtttat tatcttaaca aagttcatta tcttatataa
    13621 tatttaaatg catagacttc tttaatttaa ccatctaaat atttaattaa atattattat
    13681 atgtaatata actaattttt aaatgaatgt ataggagctg gaatccttcc tcctctttga
    13741 gtaaataatt cagatgaatt tgtatttata ggcattactg gtgctcgtcc taaaagtcct
    13801 ccaaattcta ccatatcacc tacatcacat cctggggctg gtataattct aacggctgta
    13861 gtcttattat taatcatacc tattgctgcc tcatcagcta tcatggctcc aagcgtacct
    13921 gctgtagtat ttcctggcac agctatcata tcaagaccta cagaacaaat agcagtcatt
    13981 gcttcaagct tatctatact taaagcacct ttaataacag catcaatcat acctgcatct
    14041 tcagatactg gtataaacgc tccactaagt cctccaacat gtgaacatgc cattactcca
    14101 ccttttttaa cagcatcatt taaaagtgct aatgctgctg tagtcccatg agtaccaaca
    14161 acttcaagac ccatttcctc taatatattt gctaccgaat caccaatagc aggtgtagga
    14221 gctagtgata agtctattat cccaaatgga acatccaatc tactagaagc ttcctttgcc
    14281 acaagttgtc caactcttgt aattttaaat gcagtctttt taactgtttc tgaaacaaca
    14341 tcaaaaggct ctccctttac tttctctaaa gctctcttaa caacaccagg gccacttaca
    14401 ccaacattta taattctatc agcctcacca acaccatgga aagctcctgc cataaatgga
    14461 ttatcttcta ctgcattaca aaatactact aattttgcac aaccaaaacc ttttgcatct
    14521 tttgttaatt ctgcagtttc ttttatgatt tctcccattt ctttcactgc atccatattt
    14581 ataccacatc ttgtagaacc tacatttact gaagcacata ctttattagt acttgctaga
    14641 gcttttggaa ttgacttaat tagtatctta tcaccttttg tatatccctt ttgaactaat
    14701 gctgagaatc caccaataaa atctatacct aaatcttctg cagccttatc caatgtctgt
    14761 gcaaacttta catagtcatt ttcatctgtt gcacctgcaa taatggaaat tggagttact
    14821 gatacccttt tatttacaat tgggatacca aattcacgct ctatatcttt tccaaccttc
    14881 accaaatctt tagcagaact cataatctta ttgtatattt tttctctagc tttatctcca
    14941 tcagggtcta tacaatctaa taaagaaatc cccatagtta tagttcttat atccaactgt
    15001 tcttcatcta tcatctttat tgtttcaagt atatttccta cattcactat tatcacctcc
    15061 ccttactgtc tactacagat tatgcattga attaaatatt tcttctcgct gaatttttac
    15121 atccactgat aatttcttac ctctttcaac caagtcctca accgttttct caaaagaatt
    15181 taatgatttc tctaaatcaa ctatcataat catagtaaaa taatcctgta atattttttg
    15241 tgatatatct actatgttga tattttgctt gtataattca tcactaattc ctgccactat
    15301 acctggttta tcctttccaa ttactgttaa aattgctttc ataataatat cctcccttat
    15361 ttttttataa atcttatatt tttacaaatt gaactatgtg ttaacatata tttcatttat
    15421 ttgttagtaa accacttttt atgtatatat ttaatatatt ttaataccta tacattcatt
    15481 tgtcaatttt ttttcaatat tctattattt ttatactatt tagaaaattc atatattata
    15541 tatgttttct caacaacaaa tacagttttc ataataatga aaggtattac ttagcaatct
    15601 catgattttc taaataatac ctttctaaaa attatttttt taattttatg aatttaatta
    15661 tactttttct agtatcagga tttacaatta atatagaacc aatcataatt ggtgttagta
    15721 tacaaaataa aaaaataata tcttttttta gattatattc tttagattct aatttataat
    15781 tattatttat tattccatca taatcactta tatttgactt atttttattt gtatcttgat
    15841 ttttgttaga aatactttcc ctataaggaa aagaatgttc attggtacca aatactgatt
    15901 ttgagcttaa atcataatta tcatcatttt ctgtaattga atttttattg taaaatcctt
    15961 tttctttatc agaattattt tctttattac cgtctttagg tatatcatga ggatttttat
    16021 tttcattgct atcagtactt gattctacat catctttagg agtatttggt tgtttctcat
    16081 cagaattatc attttcatta ttattatttt cattattatt gttttgattg ttattactat
    16141 cattattttc atttccatta ttcccatctt gattgttatc atcctcatct ctatccgggt
    16201 cgtctctacc tcctcctcca cggtcaccat caatatcatc attttcccgt tcatcatctg
    16261 gcgtctcttg attgtctccg cctcccatta taaatcctgg tttaaaaact atatttgaat
    16321 acccttcttt taatcctcct accactttta ctctaaaaca acgcaactta aaatcttcaa
    16381 aaaaaagtat agcttgatct ctaatatcct cctgttcttc agatatccaa ttgattccat
    16441 cgcttgaata ttcaacaaaa accttacttg cactatacgg catatcataa aataaaacag
    16501 ctacatatcc attttttgta ttttgaaatt ccagttccaa attgtctatt ggttttttct
    16561 ctataacaga tacattcaaa ataggaacta catcatcatt tattataatt ttttctttat
    16621 ttaacatttc tgttttaaac tttcctgtta tattaaaatt ttctcccttt tccaactttt
    16681 cataataatc agtaaaatcc cattctacat ctatatcaag tcgattacca ctagatgttt
    16741 ctaataccat atatttggga aattttatat tttctatttt atcatcttta cttactttta
    16801 tttcatcact aaatacacca attcccctat gacaacattt cacaatatca tagccagaat
    16861 cttcagctat agaaggtata agctcattat aatcatcaat tatataaact ttcttacttt
    16921 ctgatataat tgattgtgcc aacttattat tatctcttag taacccactt tcattgttgc
    16981 tactatatgc ttttattgaa gtttcttcta tctcaacatc acctttagaa taaacaccta
    17041 tagcatgttc tccattcact tcaatatcct tatttagtat tctaatatca ttttcagtat
    17101 aaattcctat tgcatctttt ccatttgcct ttattttttc aacagattcg taggatgatt
    17161 taatatgtaa gtctcctcct tcttcaacgt ataaggctgt cccatcagta gcaattatgt
    17221 tgtttatacc aatacttata cttccaccat tttctatatg tattactcct tcttttcctc
    17281 cttcacctat tatattcaat tcattatcat caataataaa tctcccatga ttttttatca
    17341 atattttata tttgttggta tctattgtaa aattactgtc ataaggaata taaaatctaa
    17401 actcttcatc tattaccata tcacaagaaa gagtataaac tatttcttct ttattatttt
    17461 tattttcttg aacccacctt tcaaaatctt caagacaatt ttcctcttgt ccaaaaactt
    17521 tatgtggaaa acttataaaa cacaaaacta ttatactcat aaaaataaat atctcatact
    17581 ttttcatctt acactcctag aaatttttag ctttgaaaca atcctgtcga aacatatact
    17641 tcacccacta taatttttcc acaattatca attattgtca atttatataa gtatctttat
    17701 taattgttat aaataaaaag ctacttacta aagcaaacag acgtttcact ttaataagta
    17761 gctatatttt tataacaaaa tagttataat attagaactt ccttcattta caattttttg
    17821 taaagtcttt tgcattttaa ctcttatctc ttctggcata gtatataatt tactttgtaa
    17881 ttgctctttt actaagtcat gtagtgattt accaaacata tttgattccc ataaatctgc
    17941 tggttgctct tcaaatactt ccataagata ttttatcatc tcttcacctt gattttgatt
    18001 tccaactatt ggagaaacct ctgtagatat atctgctttt ataatatgaa gtgatggtgc
    18061 attggctttt aacttgatac catattgttt tccttgtttc attatttctg gttcttccag
    18121 actaagttct tctaaggaag gagctactac tccatatcct tttatcttgg catctattaa
    18181 agcactttct attttatcat actcattttt aactttagat agtctagtta tcagactaag
    18241 cagttgataa tctccttcaa ttttaaatcc acttttttct tcaaggacat tatagaataa
    18301 atcttgcttt gtctgtaaat caatatttat aacaccttct cctagctcta cattatcaac
    18361 acctgtatct tccaaaaact ctaactcaga aaatccttgt ataatacctt ctatatccct
    18421 tattttacct atatctataa tactttgttt taatgttgtt attatgctac tttttatcca
    18481 atgatttctt tcaagacctt caacccattt tggtaagttg attcttattt ctgtaagtgg
    18541 aaaatcatat aatacagctt ccataacctc ctctatatct tcttcttcca tttcaaccac
    18601 attcattgga agaactggaa cttcatactt ttcttccaat tcacttctaa gcatactcgt
    18661 ttcctcactt tttggactta acgtatttaa aaccactgca aatggttttt taagattttt
    18721 caattcttgt ataactctct cttctggttc tacatagctt tttctgtcaa ttccagtaac
    18781 tgaaccatct gtcaagacaa ctatccctat tgtagaatgg tcttttataa cttttttagt
    18841 tcctatctct gctgcctttt caaatgtcat agcttcttgt gaccaaggag tggatactaa
    18901 tctttgtttt ccaccttcct catgccctaa agctccttca actatgtatc caacacaatc
    18961 taccattctt acttttaaag atactgtgtc ttttattttt atttctacac catctgctgg
    19021 tacaaatttt ggttccactg tcattatcgt tttacctgaa ccactttggg gtatctcatc
    19081 tcttgttcta tcctttttaa attcattgtc tatattaggt ataaccaact tttccataaa
    19141 ttttcttata aaagttgatt ttcctgttct tacaggtcca actactccaa tatatatatc
    19201 cccctgagtc cttttggata tatcttcgta tatgttatta ttcatattat tcctcctata
    19261 catctaatct tattaaatta tatttagagt taagggttaa tattcttaaa atagaaaaat
    19321 aaagaccagc aatattaatt taatttacat atagaaattt gtaaatttca atgtaaatta
    19381 aattaatatt gctggtccaa atagtagtgt attatattaa ctgactttag tagtattttc
    19441 atttgaatta ttattcatct caataagttc tttacgtctt tcttttgtca tgttcatcca
    19501 tataaatgta aacaccaatc caattatggc tactatagta agaactatga agtagtacct
    19561 atatccagtt acacccggaa atgcatccaa tatcttccct gctgctaaag gacaaagtac
    19621 ttctggaaga tatcctaatg tagcaactat tcctatagca gttcctgata ttgataatga
    19681 ataattacct tcttcaagta acgaatagtg taaaccttga attgcataca tagatacata
    19741 tattgcaaca catccaatca atagtatata aatcatagat gattttgttg gtgtaaatat
    19801 tacacctaaa agacctgcaa tcataagtat aaatccataa gatataatct ttgatgaacc
    19861 aactttatct ccaagtaatc cagaagcagc tgatgctact ggtctacaat actgagctaa
    19921 tattgagatt gcagatgcaa acaccacagt agctccaaat gcttcagttg aataaggcgt
    19981 aaaataataa aatgacatat tagctgaata tgaacaaaac attattattg ttattatcca
    20041 tgtatgaggc atttttatta ctgcaaaaaa ggcttcttta tcaaattttg ctgatacctc
    20101 tttttcatta tcctttaatt tgaaaaatac taatactcca actattacat ttattgccga
    20161 gtataatact ataatagctg taagaccaga tttatcactt acttttgatg aaatataccc
    20221 aaatagtatc aatactagag atagatgaat tgcattggta attccacgac cactttccat
    20281 aaatccaaaa gcttttcctt gttcattttc agatgctaac attctaacag cttttacaag
    20341 agctggccaa aaagtaagta ttgttgttat tccccataag gcatggatta aaaacacaac
    20401 aatatatgga ggataaagtg ttaatacaag accagatatt cctgtaataa ttaaagaacc
    20461 tgaaagcaat tttctggctg aaatacggtc tgctaaaaaa ccaccaaaaa ggtatgatac
    20521 cattgcaagt agaccataaa tacttcctaa gcttcccatt tgtgtatttg taagattaaa
    20581 tgcttgtgca aaagtatcat aataataatt ctttaaatat ggtaaagcat atataaatgc
    20641 tcctgctaca aagagtatta tcaagacttt gaaatttttc caaatgcttg gtttatttac
    20701 ttcaactgac atacttcctc cttatgtaaa tacctgtaaa ttttcatgat aattaattat
    20761 tgcttattgt attgtttatt caatatttcg gaatatttat taatatatta ggttaatcta
    20821 tcaataattt atggtgagtt atgagctaaa agtaaactct tttagctcac cataataatt
    20881 atatttttat ttatactaac gtccagagtt attagatatc tttttttcat agttcttcca
    20941 tagcattaaa cctgttatta taacaaaaag acctcctcct atcatttcaa tctcatagat
    21001 tatattatct tttaaagtct ccataactgg tgccgttgag aaaagtatgc caactacagt
    21061 tactacaagt accattctgg caatattgat acaaactgaa tcactttttg caattgaata
    21121 tggtctttct tcatttggtc ttgtctttct aagtctaata taagatgtaa aaagcaagac
    21181 atatggaaat aatgctgtta atgctgtcat agtaacaaga acattgtata tattatctac
    21241 tgatggtaat aagtttgttc ctacaagaag tagactcact attatagcct gaactattac
    21301 tgcatttgat ggtatattgt atttatttgt aacagtcaat tgcttaggaa atacaccttc
    21361 tcttacattt ccaaaaagca tctttactgg acctgctata taaagtatta atgctcctag
    21421 tactgataaa gttattccaa atgctactat tcttataaac caacttccta tacctaaatc
    21481 ttgtgcaact ttagctaatg catctagtat ccctgtagat gctgcaattt tatctggagg
    21541 aagtatcatt gttattgcta ctgaacctaa gatatagatt cctcctatta aaacagccac
    21601 agttaatata gctttaggaa agtttttctt agcattatcc atttcggtta tgaaattagc
    21661 agtcgtttca gctcctgata atgcaaatat tatagcagat atagaaacta atgaattggc
    21721 atctattttt ggtattatat tttggattgt atatatcgat gctgatggag cttttttgag
    21781 aataactaca gacataaatg ccatcactat aagtaatata gcaggaattg tagaacctaa
    21841 tgctcctgta ttcgtaaaaa attttccaaa tgccatccca cgagtactta ctaaggacaa
    21901 cagccaaaat attatgagtg aaagtgctaa aataaacatc ttattgtttg ataaatctgg
    21961 atttccaagc atatatgcaa tatttatagc taaaaatgtc aagaaagaag aataccaaaa
    22021 tatctttgat gtccaattta gccatgacac cataaatccc cacttctctc cataagcttg
    22081 tttaacccat tctccaagtc caccatcttt tggatatgtt gcagccaact ctgcacatat
    22141 aagtgatgca ggaacaaaga atataaaagc aaacaataac caaactggta tagaaccaag
    22201 acccaatcca aaactagagg cagtagattt agctatccat ctaatcccaa atagtgctga
    22261 tatattcata aatactaaat cccaaaatcc aattttcttt ttcactgtaa ttcctccaat
    22321 ttcaaattta ttcaaactca tacactatgt attgatttaa atatcaagta taagttcttc
    22381 aacacttctt ggataattag ttataattgt agcaccattc ttacctacta ttgctgtatc
    22441 tgagtgtcta aatcctccaa ctccaggtat atatatccct ggttccatag aaaataccat
    22501 tccttcttct aatattaatt cattgtcaaa tcttaaatat ggctcttcat gttctgacaa
    22561 acctaaacca tgaccaattc tatgatttga atataactct agaccatact ttgcaacaac
    22621 agctcttgct gcttcatcca ccattcttgc tggtatacca gcttttattg tatctaatcc
    22681 agcttgttga gcctctactg caatcttaaa cacttctttt tgtctttctg ttggtttgcc
    22741 tataataaaa gttctttcat tttctgctct atagttttca taccaaacct gtctactatg
    22801 tattactata tctcctcttt gaagtattct agtattagaa tctaaatgag gttgtgctgt
    22861 tctatcaatc cctgaacaag tccagtttgc aaatcctata tatgtatcag gataattttc
    22921 agaaacatat tttagtaaag cattatcccc ttcaacgtca aactccaatt cactcatccc
    22981 aactctaaca ttttctaaag acccttttat tcctatatca gataggtaac ctgctatctt
    23041 caaaaattct atttcttttg catcttttac atatctcatt tcaaatattt tacttccaac
    23101 atctttgata gcaaatgact tatcagttat atactctgaa aagcttgcag atactatatc
    23161 tttttctatt ccaacagttg ttcctttagg gtatttattt aaaattatat ctaagtaatg
    23221 tttatgtgat attccatgtt ctttcatttc tggtatttca taatacacat atacattatc
    23281 aacttttgca acttctttag catgtaattc ttctaatcca ggtactatta attctatttt
    23341 atcttgagtt actactgtta caattggtct agaataagtt attgctttaa atccactaat
    23401 ataaaattga ttgtctggtt caaatattaa agcaatttca atgctttttt cattcatgta
    23461 ctttctcaat ctttctagtc tcatattatt tgacataatg cacctctttc ttaatattca
    23521 gataatttat tcaatacaac aaggatgaat atattatttt aaaattctta atatttttag
    23581 tacttgtcgt atttgtatgt aaattaattt tatttgtgat tattttatca taatcatgat
    23641 tattttgtca atcatgattt tattttaaaa aattttttat attctagtga ttttaaatta
    23701 tctaaaatat aacgagtata cattgtattt tgtaatatta ttcttgattt atggtaagtt
    23761 taatgataaa atcacgatta tataaactat attgattatt tgtaggtgaa taaaatgaca
    23821 aataaagaat tacaaaagaa aaggatattg atgtatttca tagaagctgc tcaaaatatt
    23881 atggaaaacg aaggtataga aaatattaca cttcgtaagg ttgcagatat ggctggctat
    23941 aatagttcaa ctctatataa atactttaaa aatttagacc atttaatatc atttgcatct
    24001 ataaaatatt ttaaagatta taacttgaat atttcaagat gtatagaaaa tgtcaatgat
    24061 gaatataaga aatatataat tatgtggaaa ttattttgta agcattcttt tgataatgct
    24121 caagcttttt accaggtatt ttttaacttg tctagtgatg aacttagcta tataacaaaa
    24181 caatattatg atatgtttcc agaagattta ggtattcatg atagtgacat atctttaatg
    24241 attacaggag aaagtataaa ggaaagaaat aagatactat taaacaatct tgtagatact
    24301 ggatatatac cagctaaaga tttagacata atcaatgata taattatttc tttttatcaa
    24361 aatctattat tcttaaaaaa gaacacagga aaaaattctg atgatgatga attaactatg
    24421 aaaatgattt cttctattga atacataata aaacatgcta aataaaatta aaatatggct
    24481 gtctcaaaat agagataagc cttttttatt gttgaaataa taaaaatata tttaatttca
    24541 tataaaaatg agtgcctcgt aaactaaaaa gttcattttg agacacccct aaaataaatt
    24601 tattatttat tatacaatag tttatatatc atcacctaaa actacttctt ccatttcatg
    24661 ctttttactt ctcatcatta gttctattcc aacttcttta ggatttgaac ctttctttat
    24721 tacagaatat atagcattag ttataggcat atctatatct aatttttcag caacatcatg
    24781 tgctacttca gtagcagtta tgccttctac aaccatcttt acttctttta aagtatcttc
    24841 taaactcatc cctttaccta tgagtatacc agctcttcta tttctactat gcatacttgt
    24901 acaagtaact attaaatctc caatacctga aagacctgca aaggtagaca tattagctcc
    24961 catagcaatt ccaagtctgc tcatttcact aatacctctt gtcataaggg cagcttttgc
    25021 attatcacca taccctaatc cgtcacatat cccagctcca aatgctataa tattttttaa
    25081 tgctcctcca agttctactc caactatgtc tggattcgta taaactctaa gtttagggct
    25141 cataaacaaa tcttgtatca tctgtgctat ttttaaatct ttagaagcaa ctactacggt
    25201 agtaggaata tcccttgcta cttcttcagc atgagatggc ccagatagta ttacatatgg
    25261 attttgtggt aattcttctt cacagacttg tgataatcta agcccagtcc ctttttcaag
    25321 acctttagct acattaacaa taacttgtcc ttcttttata aatggtttta ttttttggca
    25381 agtacttctt acagcctgtg atggaactgc taaaactatt accttacaat cttttatagt
    25441 tttttctata tcagtactta tagttatatt atttggaaat aatactcctg gtaaatagtc
    25501 tgtattttct ttagttctgt ttattttttt agcttgttcc tcttttcgag tccacatgct
    25561 cacatcatta cctttttttg caagccctaa agctaatgca cttccccagc ttccagtacc
    25621 taatacacat actttctcca tgtttaacac cacctgagta taatatattt actattcttt
    25681 cttttgacca atttttctct ctgtcccatt taataatcgc tttatatttg ctctgtgatt
    25741 atatattact gatgcagtta agaacagagc tactattaaa ccagctttat tttttaccat
    25801 aatcataaat attggtgaac atcctattcc tacaacagaa cccaatgaaa catactttgt
    25861 tatagctacc accaatataa atactgctag acacattagt gttattactg gatttacagc
    25921 tagcattgcc cctagtgaag ttgcaacacc tttgcctcct ctaaatccta aaaccgctgg
    25981 ccaattatga cctgctacaa cacatataac tgccagatat ccagctaaaa gagtatctat
    26041 tccaaccaat cttgctgcaa attctgatat taaaacagcc attacacctt taagaacatc
    26101 acctaaaaat gtcattgctc ctgctctttt tccaagtgtc ctaagtacat ttgtcgaacc
    26161 tgcattacca gaaccttgag tccttatatc aaccccagca attcgctttg caactatgta
    26221 tgaggtagat atatttccta aaagatatgc aactacagca ataattatat aactaaatat
    26281 ctccactgaa tttcccccct ttagttatat tttaagatat ttatttttta tttttttgtc
    26341 tatactcaaa ctttatggaa gttccttcaa atccaaaatt ttctcttatc ttattctcaa
    26401 ggtatctttg atatgagaag tgagttaaat ccttatcatt tataaacaat gtaattttag
    26461 gaggtctaat gtcagtttga gtcccatagt aaatttttaa tcttctacct ttgtcagatg
    26521 gtggttgatt taacattaca gcttctccaa ttacttcatt cagtgctgaa gttgatattc
    26581 tcttagaatg ttcatttgac acatattcaa ctgtatctag tatcttgttc attctttgat
    26641 tagtttttgc agaaacaaat acgattggag catacatcat aaatggaaat ttttctttta
    26701 tatcttttgt gtaattactc atagtcttat tatctttctc tattaaatcc catttattga
    26761 ttacaaatat gcatcctttt ccttcatcat gtgctatacc tgcaaccttt gtatcttgtt
    26821 ctgttacacc ttctaaggca tctattacta tcaatactac atctgctcta tctacagcac
    26881 tcatagctct tattacactg tatttttcta ttgtctcata tattttactt tttcttctaa
    26941 gccctgctgt atctattaga agaaatttct gtccattttt ttcaaaataa gtatcaattg
    27001 cgtctctagt agttcctgca attggactta caattactct ttcttctcca agtatcttat
    27061 tgagtataga actttttcct gcatttggtt tacctgttat agctactctt attatatctt
    27121 cttcatactc tgtatctaat cctgctggaa aattttcaac tatctcatct aataaatctc
    27181 caaatcccat actattggct cctgaaactg caaaaggcgt tccaaatccc aactcataaa
    27241 aatcgtatat attatcaaat tgactttgac tatcaatctt attaaccact aagattactg
    27301 gtttttttgt ctttctaagc aattgtgcaa tttctttatc agcagctgtt attccagctt
    27361 taccatcaac tataaaaagt attacatgtg acatgtccat tgcaagcatg gcttgatttc
    27421 tcatttgaga taatattata tcttcgctat caggttcaat tcctcctgta tccactaatg
    27481 taaaatattt atctaaccat tctacttcag caaatattct atctcttgta actccaggtg
    27541 tattttcaac tattgatatc ctttttcctg caaatttatt aaatattgta gacttaccaa
    27601 cgtttggtct cccaactaca gctacaactg gcctacttat actcatttta tttctccttc
    27661 catcttttgt tttctatttt aaaattttat ccacaaaatc ttttccttca ttcaaacaag
    27721 ggataatttt tattctcata agttcttcta attgatttaa ttcaatatta tctaagaaaa
    27781 tttcttcatc tgcttttagc atacttcttg ttatataaag agcttctcct aattctttat
    27841 cttcaagttg ttcttttaaa tccttagctg ttaaaagacc tgatacagtt attgtctctc
    27901 caaaaaactt gtttttgatt tcatatacat ttacttgaag atttttaaac ttgtccatca
    27961 tagcatctgc catactctgt ataaattcat atgcagaatg accagtagct attgatactt
    28021 tcttctctct ttttagaatc ttctcaggca atatatttag atactcttca atctctgttt
    28081 ttaacttacg tatcatacct acaccatctt caaactgtaa aaaaccttca tattcttcat
    28141 atccaggcaa ttcactatta gatagtatat aaaattcatc tgataaaaat gcaaatcgag
    28201 tacctaattt ttcaagatat ttctgttgta tttgatgtat ttgctctata gttttacttg
    28261 cgctcttatc attaaatata tttaattcta ctagattttc tctatgcttt gtaatcccaa
    28321 ctggaactat cgcaacactg tttacatacg gataaagttg agctagttct tttattgttc
    28381 tatccaattc ctttccatca tttatacctg gacataaaac aatttgacag ttcatttcta
    28441 tatgagtatc tgccaatctc ttcattatac catataattt tcctgcaaat ttattgctta
    28501 tcattttttg tcttaactct ggattagttg tatgcacaga tatatttata ggacttattc
    28561 tatattttat aatattattt atatcttctt cactcatatt tgtaagtgtg acaaaatttc
    28621 cttgtaaaaa agataatctc gaatcatcat ctttaaaata aagagtttct ctcatacctt
    28681 ctggcaattg gtctataaaa caaaacatac acttatttct acagctttta gcttggtcaa
    28741 ttatcggatt tgtaaattca acacccaaat cttcatcata gtctttttct atctcgtata
    28801 tgtagacttc gccatctttt ttttgtattt ctacatctaa atattcatca cttagtaaga
    28861 atctatactc tattatatca tgtattggct gttcattcac agatataagt aaatctccta
    28921 cctctatacc tatttcttct gcaatactat ctttataaac cttacttata atattattta
    28981 ctttttttac atttacttcc aatattttca ccactctttt gtattcatca atgcttatta
    29041 taatatattt taatatgcaa tttatttatt tattatattt tatgatagca tttttaagca
    29101 tttcatgtca taataacaca ttttaattct aatagtcaat ataagtaaat agctataaaa
    29161 tagattagaa caaataatat tcaaatttaa gtttcttaaa aatttgaata ttatttgttc
    29221 ttaaaaaaat tttataaatt taatgtttta ctatgattaa cgtcccattt gtcatatcat
    29281 gaaccattcc acctacacac tttgacatca acaatgcctt attttgtaag tctttctcat
    29341 catttgcata aaatattgga caacctccac catttttcat attcttatca agtgtaacta
    29401 tagctagggt atattcattt aaacctatat ccattcccat cttatgcact tcctttcttt
    29461 cctattttat aattttttag tgataagttt tttcctttag aacttgaaag tattgggcat
    29521 gactttacgg cttccattac tttttccaca ttcttatcta ctggtaaata agcaatcatt
    29581 atactttcat cctctttatt ccttctaggt aatggtacga atgaaggttc tccataatct
    29641 ccatataaac ctaaccttga atatatattg tatactattg cctgtctttg acctgagtca
    29701 aataatatcc ctgcattagc ataatcttta tttttaggaa ttatttgtat tcctacacct
    29761 ctattaatat atacttctct atcacttttt agacctatat ttgttattcc ctttaaatct
    29821 cctaccttca atatagactc atctacaaaa ctaatctcag caggcattac atcagctatg
    29881 tctccaatac tttttctagt aagaagtttt tttaatataa aggctaacac taatccacaa
    29941 attgaactta ttaccaaact cattccttca cttattttcg tttctgcaag tattacataa
    30001 tacaatccta ttgttaaaaa agatgtaact atacacatat aatttcttac ttcataggtt
    30061 ctagctatct cttctatgaa tgcagctcct ctctttacaa gttgaacttc ctctaagttt
    30121 tgcaatgtat ttcttctatt tgctctcact tgtctaaatt gctctgctgc tagggataaa
    30181 aaagttatag atgtataaga acgatttata agagctggaa ccaataatgc tcctaatgaa
    30241 gaagcgacga atgctaatat aagttgagat agtaaaacat ttggttgcgt tggatactgt
    30301 ttttggtcta aattcaacat tataattcta gatgttatac caataataac agcaacaaaa
    30361 aatactctat ccattaaatc actcctaaaa tatttaattt gaaattttac atgtaaatct
    30421 acaaagtctt tctagaaaaa tcactctgct ttccttttga tgtttctatg ataggtgtac
    30481 ttttcactac atctatcatg acatctatat ctttgagtat tggcatatat ggtataacca
    30541 cagtactttt ttctaaattg gtcttagata tagcaacaat atcaacctca tctacatctc
    30601 tatctatccc catatgaata aatagattat gtattatagc ttgtctttgc cctatatcat
    30661 ttactattcc aaatgcacct aaatctcttg gtataacttc tatggccaga cattcatttc
    30721 tatacttttc tcttgtactt tctagaccta tgttagttat tattacacca tttaccatta
    30781 aaataggtcc ttcaaaactt atttttgcag gaactatatc tgctatatct gctatactgt
    30841 tccttcttaa aaatcttcta aaaatcaaac caacaatagc tcctgacaca atagctagga
    30901 ttgtgcaata aaaaaagcta aggccatact ttcttgcaaa aacgatataa actatcgaag
    30961 ataccagtgc agaaaaaaga cttatataac ttctagattc atacgtagat gctatttctt
    31021 caacgtaagc gtctcctttt ggtactaatt cttcattatc gatattcttt agtgttattc
    31081 tttcttgttc tgcaagacct tgaaactgtt gtattgctac tgcaaaaaat gtcaaggctg
    31141 caaactcctt atctataagt gcaggtaatg ctatagcacc taacgaagct gaaagcccag
    31201 atataattat ctgttctaaa taatcttgtg gtcttgatgg gtattgtttg tctgtaactc
    31261 ttaaaactaa tcctctacat aatattccta caactaatgc aactataaaa gagtgtctaa
    31321 ataaatttgc attcaataaa ctttcttcca ttttctcagc tccttctctt atatagtttg
    31381 tgcaaaaaac aaaaaaaatt cccttctatt taaggaaatt tttttgtaat attgtaacta
    31441 taaattcgct accaatgcca agttcacttt ttacttttat acatccattt agtgatttga
    31501 ctatatgctt tgttatagcc aatcctaaac ccgttcctcc tacatcccta cttcttgctt
    31561 tatctaccct ataaaatcgt tcaaatatgc gttctacatc ttcttttggt atccctacac
    31621 cattgtctat aactttaatt acgatttttt cttcatcttt taacactaca acttgtacaa
    31681 tcccattatc ttgggtatac tttatagcat tatcaactag gtttaagaac acttgcttta
    31741 tatagtcttt attggatagt atacttattg atttatcatc acatttatat aaaacttcta
    31801 tatttttttc actagctgaa tgttgagtta tttcatatac ctctttaaat acatcatata
    31861 ctacaacatt ttccataaga agagtatctt tattttctac aaatgataac agcaatatat
    31921 catcaatcaa cctttttaat ctatctgatt cgctttctat tatttctaag aatctatttc
    31981 tagtattcac atctatattt tcatttgact ttagtgtttc aacaaaccca cttatagatg
    32041 taagtggtgt tttaagttca tgacttacat ttgcaacaaa atcacttctc atattttcaa
    32101 gtttaacttt tttagtaata tcttctatat ttactatcga acctattatt acgttgttag
    32161 tattctgaag ataaactggg tctactttta tcttatatac gatatcatct ttagtagtaa
    32221 tattagcagt ttcattatct ttagaaccaa taaattgaag tatttgtttt aatatttctt
    32281 cttcttttat tacaacatta atattatgac cttcaactaa ttgatttggt tcactcttta
    32341 tcatttcttt tgcttcatca tttatcagaa gtatatttcc atttacatct atagctaaaa
    32401 ttccatgaga tatactcttt aatatagacc ttagctgtaa atgtttatac tgtacttctt
    32461 ctaaggtttt atccatattc tttatcatat gattaaaatt tctagctaat tcgccaagtt
    32521 ctcctttggc tgatatgtta agcctagcat gatattcatt attacttact ttttttgaga
    32581 cgtatataaa ctcttttaaa tatttcctta atgttattgt gtatcttaca gctaatataa
    32641 caatggctat ggacaacata acaataaaaa aatatatact atttaaatct tccacaagat
    32701 aacactccta aaatataacc tttttaatct attttgtatc ctacacctct tatagtctct
    32761 atatattttt ccgatttatc ttcatcttct attttttttc ttaaatatct tatatgaaca
    32821 tctactgttc tagtttctcc atagtattca tacccccata ttttatccaa aagataattt
    32881 cttgaaagta cttttccttt attttgaatt aataatttta acaattcaaa ttccttaaga
    32941 gttaactcta tcttttcaga gcctttggta acttcatgtt ttgataaatc tagttttaaa
    33001 ttacctgtag taagtatatt gttttcttca cctaagctag tcaaattata tctccttaaa
    33061 acagaactaa tcctagctaa caactctttt atactaaatg gcttagttat atagtcatca
    33121 gcacctattt caagcccttc aaccttgtca ttttccatat ttttagcagt aagcattata
    33181 acaggaatat tttttaagtc cttatcactt cttatttttc tcaaaacttc tattccactt
    33241 atatttggta acatccaatc taaaagtatt aaatctggtt taatttcctt agcctttata
    33301 aatccgtcaa atccatcata tgaataacta acttcgtaat ttgacacctc caaattgaat
    33361 tttaatagct caactatatg catttcatcg tctataacaa gcactttagt attcattaaa
    33421 aagctcctct ctattttatc tgtagtaccg ttcctattcg ttctgatgcc ttattatgtt
    33481 ttctataaga gtggaattta tctgctctac aactagtgca taaatttaaa tttattatat
    33541 tttctttttt aactccacta catctaagca tatactcatt aattttccat aaatctaaat
    33601 agtaacttcc ctcttttatt atatagaact tttcatcatt atttgtaaga attgtgttaa
    33661 atttttctac taaatcttca gacacttcat aacaacatgg tccaattgat ggcccaatta
    33721 tacatactaa atcttcagga actgttttat agcattcaga cattaatttt attgttttat
    33781 gacctatatt actaaaagtc cctctccaac ctgcatgagc cagtcctatg gctttatttt
    33841 tagcatctat aattgatata ggtacacagt ctgctgtaaa cagtaaaagt ggtacttttt
    33901 ccaagtttgt tataagtgca tctccatctc ttcttttccc tatagtatct ttattgacta
    33961 tattaactat atctgaatgt atttgagaat tactagttag attttcaaat gtcaaatcac
    34021 attctttaca tacttttaac atatcttctc tattttttgc atctatattc ttacatgtta
    34081 taactagatt tacagaattt aatttttcat caagttcttc acaattacca cttaaattaa
    34141 catactcttt aatatcttgt acatttttta tatttatata atctttcatc atttgtctcc
    34201 tttttctatc agtatacttt taagttcatt tacaaaagta tttatatctt taaattgtct
    34261 atacacagaa gcaaatctta cataggcaac ctcatctaac tctttcaatt tatccattac
    34321 catctcaccg atttttttgg tttcaatttc ttctataaaa catttattta tttcattttc
    34381 aatatgtaca acaatacttt caatctcgtc aatagaaaca ggtctttttt cacatgcttt
    34441 tagcaatcca tattttatct tttctctatc aaaatattct ctactattat cctttttaat
    34501 gaccattaaa ggagttgttt ctattttctc atacgtagta aacctctttt tacatgattc
    34561 acactcacgt cttcttctga tagattttaa atctgtatgt ctagaatcta ttacctttga
    34621 ttctttataa ttacaataag gacattgcat ctatattcct cctttgttta actatatctc
    34681 aaactcatct aagcttaatt ctgaacctat atttactatt attgtatcac aacctatttt
    34741 aagtatatca ttccaaaata taacttcttc atcccctcct cttgaaaaaa aacttctact
    34801 tccatctcca aaaatggtaa atgagactac cttaccttcg tgaatatcca tgtctatgtc
    34861 tattataaac cccatccttt ttccattttt tacatttata atttcttttt ccattatatc
    34921 agaaactcta atcatattgt actcaccctt tctttaaaaa taaatatgta tcgaagtaaa
    34981 aaaaaatgac ctatataggt catttaaatt aaaataatgt ttacagtcca tggattactt
    35041 atacatattt tctcatattc tttaaagcat ttttttcaat tcttgatact tgtgcttgag
    35101 atataccgat ttcatcagca acttctatct gagttcttcc tttgtaaaac cttaaatcta
    35161 ataccaactt ttctctacta tttaatttct taatagcttc tttaagtgat atttcttgca
    35221 accaattttc atcagtatct tttttatctt gaacctgatc cataacgaat attgcatctc
    35281 cattgtcttg atatactggg tcaaacagag atattgggtc ttgtatggca tcaagtgcca
    35341 ttacaactga ttctacttct aattctaatt cctttgctat ttctgatact gtaggttctt
    35401 tagaatttgt tctaattaat ctttctctaa cttgtagtgc cttatatgct atatctttta
    35461 atgacctact tactctaatt gggttattgt ctcttagata ccttctaatt tctcctataa
    35521 tcataggaac agcataagtt gaaaacctta cattttggct taaatcaaag ttatctatcg
    35581 cttttataag accaatacaa cctatttgaa ataaatcgtc tatattttct cctctgttgt
    35641 taaacttttt tattacactc aagactaatc ttaaattccc tcttacaaac tgttgtctgg
    35701 cttcttcatc accatttttt atttgtaata aaagttcctt catttgctta tttttaagaa
    35761 ctggaagttc tgatgtatta acaccacata tttcaacctt attaacttgc atatattttc
    35821 agtcctctct caaaagtatt catctattga aattatttac atttccaatt tttttatact
    35881 ttttaagaag aattaattta gcattataca aattttttca tttctttttg caaccttgag
    35941 attattttct tttcaagtct agatatgtag gactgtgata ttccaagcat tccagcaact
    36001 tctttttgtg ttttttcaat acctttatca ataagaccaa atcttaattc cataatttgc
    36061 ttttctctgt cacttaatct gtcaagtgcc ataaccaaca aatctctatc tatttcttct
    36121 tcaattattt tgtatatttc atcattttct gtacccaata catcagataa taaaagttcg
    36181 tttccatcta aatctatatt taaaggttca tcaaatgata cttctgtttt ctttttatta
    36241 ttttttctta agtacattaa tatttcattt tctatacatc tcgaagcata agttgccaat
    36301 tttatatttt tattcagttt aaaagtatta actgctttta tcaaacctat agttcctatt
    36361 gaaatcaaat cttctacatc tatacctgta ttttcaaatt ttctcgatat gtagactact
    36421 aatctaagat ttctttctat taaaattgac tttacacttt catctgtttc tagcttttgt
    36481 aaaagctcca tttcttcttc tggtttcaat ggaggaggaa gtatatttgc ccctcccata
    36541 tagtatattc cctttggctt tattattttt aatttaatac ctaacatagt aataaagctt
    36601 attattcttt ctttcaatcg taacattcct tgtccccctt aaattttcac ctaaaatcca
    36661 ggcaatatat ttggattcat tattgctcca tattctctat cattaaaatt tgaaatacca
    36721 agtattatat ttcctatctt ttgctcatct acttctatat agtcagcttt taagcctatt
    36781 attatgcttg tcttattact tccagcatgt ttataaggta ttattctaac tctacctgaa
    36841 gttttatcat ctaaacttcc tataatttct tgtgctttct tcatatctat atcttcataa
    36901 ttaatattta atagtttttc tggaataaat ccttctaaca cactagactt aactattatt
    36961 acatcacttt gacttattgg gtcttttaac aaattaccac tatctactaa agccttacag
    37021 caaaaacttt tttctagtaa gtttatattt atagtctttg taagctcctt aatgtactta
    37081 agcattttca aatctttata aatgtatttt aataattcac aactcacata agtacatgca
    37141 attaagaaag atatttttaa gtgacttatt cctgtaaaat aaattataaa ataagtgctt
    37201 ccagaaataa aaatgtttac tagataaaaa actaacgcta catgaacata atcttttttg
    37261 ctcttacttc taaatgaaat taaagttatt atactcatga ttattatttt aaatggtaca
    37321 gtaaatagta tattcattga tggatacaaa taagcaacag aataggcacc tcctaaacta
    37381 gcccccaata acttattttt tttaggatta tatcttttag taagaataga agtacaactt
    37441 ataataatat aatttattac taggttttct ataacatagt actcaatata caccatgtat
    37501 ccccccttaa aacctttaaa catattataa gatagtatgt acaaaatttt atgtcacttt
    37561 tgatggtcga atccaaaaca aattctattt aaaaagaaca atgatatatg tcttatactt
    37621 tatgtcgtca aaagatattt ttttaatgcc aaacaaatag acttttttgt agaatccaat
    37681 atatctaggt agaagttatt cgctctactt ttggtatatt tacaaaaaaa gactacataa
    37741 tttaataata tatcaagtaa atcttttact tatataaaat caaattatat agtctgtaaa
    37801 ttcaaatata aattattgtg ctagtaatcc atttgcatat tttgcagatt catttagtgc
    37861 ttgttctgga gttatgtctc cagctatcat catttgaagt tgtttccata aatattcata
    37921 tatctctact ccatgaggtc caactgtcaa tggtctatac atatgtttat ctcttgtcac
    37981 catttcttca aatcttgggt ctccttggta tttttcacct ttagcaactg gagctctagg
    38041 tatttctttg tggaaagctt ccattgattc tttgcttagc atatactcaa gaagtttaaa
    38101 tccaagttct ttatttttac atgctgacat taatgttaaa tcatctactg aagcaaaact
    38161 tccaactgtc ttatcttcta atgatgttac atatccccag tttaaatccg ggaatgattt
    38221 atcaaatgta cttgtagcag cagaggatag cattatagta aatgcagctt ttcctggtcc
    38281 aaatacactt tcaatcattt cattactatc ttttgataaa gcatcttctg gaatatactt
    38341 tttcatatca tttataaagt ttgcagcttt caagccttct gcattattaa atttaactga
    38401 ttttaaatca tcattatata tatctccacc agcttgccat aaatacggat accagttcca
    38461 gtttaagctt ccaaatactt tagaacccca accttgtgca agaccccact ggtcgatttt
    38521 tccatctcca tcagtatctt ttgttgcttt ttcacagatt cttttaaaat catcccatgt
    38581 ttttggaggt ttttctccta atttttctag tatatcttta ttgtagtaca atacgcctgg
    38641 attagcagct tctataggta aaccataaat accacccatc atttttgcat catctaagta
    38701 tagataatta tctgaagttc ctgatttttc taaataaggt gttaaatctt ctaccgcacc
    38761 catttctata aattgaggaa acatctctgc atacatgtat cctatatctg gaccttcacc
    38821 tgcacttatt gctgttgcat atttttctgc gtagttatcc catggtacta tctgaaattc
    38881 aatttcacag ttattttctt tttcaaattt ttcaaatatc ggcttccatg catctgcatc
    38941 atggtctgtt aatggtgcag accatacaac taatttttct ttaccctctt tgcctttaga
    39001 atcttctttt tttgaacatc ctgcaagaat tgatactgat agaatcccta ccataactaa
    39061 agaaagaacc ttttttagtt taatcatgct taatcccctt tcagacttta atttgtcttt
    39121 ttcctcaaat gtttacattt aattcagatt taatattaac aaataagatt actcttgtct
    39181 aatcataaaa aactatacta actcctataa ctatattttt tttctatacc tattagattt
    39241 aattcatact aaaaacttac tttttaaatt ttatcaatat ttaaaaattt ttttaagtct
    39301 agccacaaac taactatcta aattgagcaa aataaaaaga agtctattga cttctttata
    39361 agcttacata ttatcttctt cttcttagga atgtaggtat ctccatgctc tcatcatcat
    39421 cttcttcaat tatacttgcc tttttaggag gctctatttt ttcctcagca attacttctt
    39481 cttcaatttc ttttacagct tgttttactg ttgtatttaa tgaagaacgt atgcttgatt
    39541 taggtttagt atctaaatct aaggcaccat tttgtaatcc ttcaaatcct gtcgcgatta
    39601 ctgtaatcat tatttcatca cctaaatctt cttttataga agctccaaat ataacatttg
    39661 cttctgggtc acaggattcc ataactaaag ttgatgcctc attaacttca aataatccta
    39721 agttaggacc acctgtaaca ttaagaagta ctcctttagc tccttgtatt gatgtttcta
    39781 gaagtggaga ttgaatagct tgtcttgcag catcaattgc tctagtttca ccactagcac
    39841 tacctatacc catatgagca agtcccttgt ctttcattat agtagtaaca tctgcaaagt
    39901 ccaagtttat taacccttca actgctataa ggtctgaaat tgattgtata ccttgtttta
    39961 acacatcatc tgcaactgca aaagcatcta acattgatgt atttttttgt actatctgta
    40021 aaagtctatc atttggtatc gttataagag tatctacttt tgatttaagt tctgctattc
    40081 caccttcagc gttcttcatt cttatcttac cttcaaatgc aaaaggtttg gtaactacac
    40141 ctactgtaag tatccccatt tcttttgcca agcctgcaac cactggagca gctcctgtac
    40201 cagtaccacc gcccattccg gctgttacaa agaccatatc agctccttga agtaacttta
    40261 ctatttcatc tttactctcc tcagcagctc ttttccctac ttctggattt gctcctgctc
    40321 caagtcctct agttaatttt tctcctattt gaactttata ttcagctttt gatgtatata
    40381 aagcttgttt atctgtattt actgatataa attcaacgcc ttttagttga gcttctacca
    40441 ttctattgac agcattgttt ccaccgccac ctacacctat tactttaatt tgtgcacatt
    40501 cttctaattc tacgtcaaag tttagcatca taaaccccct tctctcatga ttcaaagtta
    40561 tcgtatgtat taattatatc attttcttct tttattttgt taacctttaa gtaacatatt
    40621 ccacaaaaac gtaaattttc ctttttaaat ttcttactta tattgtcaaa tattactctt
    40681 taagtataaa ataatttacg tatatattaa atgctcttta atttttacgt tttttgttca
    40741 taatattact aataatatat cgtctaatta tagataagtt attaaacaat ctcataccaa
    40801 aaactaatac agcaacatag taaagagaaa ctcctaactt ttctcctata taagtcaata
    40861 atatagccaa tatagcatta cctatgaaac cagtaataaa aattacatta tcatagttat
    40921 cttcaaaatt ggattttatc gctccaaaaa tagaatccat tattgcaaga atagctactg
    40981 acatatatat ggaataatcc atagaatatg taaatggaat atagtaacct actactacac
    41041 caaatgctaa tcctcctaaa acccataaca taaatcttca cctctttatt ttactttgaa
    41101 acacaactac attacaatcc tttttattaa ttttttttac tttttcttta tttttttgta
    41161 tttttcgttt tattttgcaa ataaattcac tttatttcca ttattttcct gaacttttat
    41221 attaattcca tatatatcct taagagattt agcatatgat tctggagagt ttatagctgc
    41281 actcaataaa tctatatctc ctatagcttt tattataaat ggttgtccat atgtagtatc
    41341 atttactgta atagtagctc ctgaacattt tatcttactt gttgctagta ctctttctcc
    41401 atttatagaa attgctcttg ctccagcctt ttttaaatca tttaaaaccc tcaaaatatc
    41461 tatatcatgt ataatcaaat cgtttggatt ctgtccgtct tttaactctc tctcactatc
    41521 cctcatagta atagttatgc ctggccctgt aacagttgaa taaccactta attctttaac
    41581 ttcttcaagc tctttttgca taagtgtttg gatagattca tttccattct tttttgcttc
    41641 tttatattca ttcaaagttt ttttgttctt tttaagagtt tcttttaagt tttttatttc
    41701 tttttttgta ttttctacct cactttcaat actttcagtt tgacctaatg ttatgtatac
    41761 tctatttggg tctagtgatt ttatataagt tgatatgaaa acgcctaata tgaatgctcc
    41821 taatataact attttctttt tcatactatt atttcccctc tgctttgttt atatcactat
    41881 gaccatttat ttctatattg tcttctttgg tcacttttac tttggcatta taatatgttt
    41941 ctatttccca gacaatacca tactttatat taagtgccga agccaatgta tcagaatcac
    42001 caattgcttt tattataatt ggttctttta tgacagtgtc attcatataa agcttgtctt
    42061 cttttaaata catatatgta ttagaaacta ttctctggtc gtttatagat atggcacttg
    42121 cttgtgctga atttaattta tttatcattg ataataataa atcataatta tatagtatac
    42181 tatcattttt atcagagttt cctaagtctt ttgtagaatt tatctcaatt tttatgcctg
    42241 ttccttcaac atctgtatat ccagctaatg tttcatattt ttctatttct gacttaagta
    42301 tgtcttgttc tccttcattg tttttgcctc tataaatctc tatatctttc ttcacactat
    42361 taacttctga ttgtagattc tctttttctt tttttaaaga tttaagttct tctgtaagtt
    42421 gctctcctct tttaaaagtt gtcatccctc tattttctat attaataatt ttaagttgta
    42481 aactaacaag aacacccaat acaatacttc ctatgattat acttttattt ataaatcttt
    42541 ttttcaatat atcacttcct tacaattgtc tatccttctg gactatacac tgccaacttt
    42601 ccatagttaa gatttatagt ccctccatta gtatttttat tttgcaggtc aatcagaatt
    42661 ttacttaccc tagatatatt ataatctaag ttatcatcat tagaaagtaa gatttttata
    42721 ttacttcttg taagcatttc tatattgctt tcttttttta agtttacata atttattttt
    42781 ctataaattc cttcttcttt taaataattt aaagttttaa atacattctc cttagtttta
    42841 taatttttaa attttataga tttattatca tcaatgctat aatctacatc tactatcaag
    42901 tcttttttac tttcattaga acctcttagt tcttccaata aatttccttt tttatctata
    42961 taacaataat tatcttcatc ttttaatact gcaaatattt ctttttcttt taaacttata
    43021 attatcttat tgggaagttt tctttttatt tccacatttt ctatatatgg attttttata
    43081 agtttatttt tcatatcttt aaaattgtat gcaaaaatat tttcatttag atttacattt
    43141 agttctttca ttatatttga tttggtcacc ctcttatttc caattacatc tattttcttt
    43201 acatcaaata aatcactttt tacaattata catatacaaa aagataacat aagtataaaa
    43261 actaaaacta tcatgacatt attcgtattt aactttcttc tttttttcaa atataatcaa
    43321 cccctttggt taataaatac acctttagag cttgtttttc ctacaaaaaa attataatat
    43381 ttaaagtcaa atttttgcat tttattacat aacatccaaa taaggtatta aattttataa
    43441 ataaatcaat atttcatcaa aataaacata gcatactaca tactatttat atatttaaat
    43501 ctgtatacat acaaataaac ccctaattta taataattta gaaaatctaa tatattataa
    43561 attaggggta aatatttact tatcttttct ttattcctat taccttagct tgaccttcta
    43621 tacttggagt cgtttctttt tttatttctt ttacttcctt aataactttt tctttttttg
    43681 tttttttaga agttgacttt tgtgttgaat tgtatacttt cattatctca tcatatataa
    43741 ggtctatggc ttcaggctta cctatagttt tacttgcatt tgccatatca accaaaagtt
    43801 ctctatctcc taaaagttta aatactgctg tatttaggct ctctggagtt agattttttt
    43861 ctaaaatagc aattcctgcc ccttgttttt ctatactctt ggcattatac tcttgatgat
    43921 tttcagcagt ataagcttta ggtattatta tagatggctt tccaagagcc gttatctcag
    43981 ctaatgaaat tgctcctgcg ctacctataa ccaaatcact tgctgcaagt gcatttgcca
    44041 tatcttctaa gtaaggcaca actttttggt atggtttaag atttatatcg ctaatacttc
    44101 ccataaattc atcataatag cttttgcctg tagcaaatat aaaagctata tcttcattta
    44161 ccatattctt tattacaagt ctcattgcat catttatttt tcttgaacct ccactaccac
    44221 catagcaaag taccattctt ttttcgtcac ttattgatag attctttctt gcaatatttt
    44281 ttcttgataa taaaatttct tttctaactg gatttcctgt aaaaactaat ttatcttctg
    44341 ctgcctctgg aaatctttta tgcgaatctt caaaacttgt aagtaccttt gtaactgttt
    44401 tagataaaat cttattagtt acaccaggaa aagaattttg ctcatgtatt atggctggta
    44461 ttttccccat agatgcatta aataaaacag gtccactaac atatccacca gttcctataa
    44521 ctatatctgg tttaaatttc ttaacaatct ttcttgattg ctccaaacct ttaaaaagct
    44581 taaaaactct ttttacatta tcgaaatcta tttttcgctt aaagccttga actgttacag
    44641 tctttaattc aaatccatac ttaggaacta tttctgactc tattcccttt tccgtcccaa
    44701 caaaaattat ctcagcgtct ggatgctcat ctcttatttt gtttgcaata gctatagctg
    44761 gatacacgtg ccctccagtt ccaccgcctg ataataaaac tttcatcaaa tcatcatctc
    44821 ctgttaatta attttgacat gctttgagat gtttaacact attcctacag ctcccataaa
    44881 gatggtgagt gaagttcctc cataacttat aaatggtagt gctacacctg tagctggcat
    44941 tgatgatgtc gcaactgcta tatttaaagc tgcttgtatt ccaatttgtg cacctatacc
    45001 tataacaacc atacaagcaa atacattaga agtcttcaaa gctattctta cacatctgta
    45061 gactaaaact acaaatagca taataactat tatacaacca atcaatccta actcttctcc
    45121 tattatagca aatataaagt cattttgtgg ctctggtata taaaaatact tttgtttact
    45181 ctttcctaag cctagaccaa acaatccacc tgaacctagt gcatatagac cttgtattac
    45241 ctgatacccc ttaccaagag ggtcttgaaa agggtctaaa aatgatgtaa cacgactaag
    45301 tctatagggt tcagcaatta ctaatgcagc aaataaagca gccccactcc ctatcatagc
    45361 aaatacaatt ttcatatcca ttccagcaac aaatatcatt acaaaagtaa ctagaattac
    45421 agtacctgct gttgacaaat ttggctgaag tattatcaac gcaaaaaata tccctggaac
    45481 tacgagaagt ggtacaacac cttttgtcaa agattttatt ttatcataat ttttttcaat
    45541 taattttgca gttaatatta ttgtagcaaa ctttgcgatt tctgcaggtt gaaatgttaa
    45601 agctcctatt cccaaccatc tttttgctcc gtttgcttct atccctaaag gggtcaaaac
    45661 taacaataat aatactactg ctatagctcc aattgctgta gcatttttct tccaaaaact
    45721 ataatctatt cttgatgtaa taatcataac tataaatcca agtacagcat atattacatt
    45781 cttttttaga aaatagtacg catcattgtg tttaaatgaa gattgtataa aacttgcact
    45841 aaacaccata actattccta caaatactaa aagcatagta gtgtaaaaca ctactccatc
    45901 aaattcagtc ttcatcctta tgtcaatttg ttttttcaaa ttttctttag gcatccaact
    45961 aatctacctc cattcttcca tttaagtaag tttagaggta agagattatt ttaaattgtt
    46021 tacattatct ttgaagtctt ttcctcttac ttcaaaactt tcatacatat cccaacttgc
    46081 acaagctggt gaaagaagca ctatatctcc actttttgaa atttcataag aagtcttaac
    46141 tgcttcttcc atatctttta ctatatgtat gtcattaaat cctttatttt tagcacaatt
    46201 ttctatattt gatgcagttt ctcctaataa gaccaatgat ttaacatatg actttgcagt
    46261 ttcaagaagt tcatcaaaat tacttccctt atccatcccc cctgctataa gtattatagg
    46321 tctatcataa gactgtatag cttttatagt ggaatctgga ttagttccct ttgaatcatt
    46381 tacatatatt acattgtcta aatttcttac aaactcttgt ctgtgttcca ctcctttaaa
    46441 tgtcataagt acatccttta taacttctaa atcaattttg cagacatatg ctattgctat
    46501 agctgccata caattttcta agttatgacc tccaggtaaa cttacatcat ctttatttag
    46561 aaatttgatt ttatcatcta tatctattat tatgtcatta ttttctaaat atactcctcc
    46621 atttactttt ttagttcttg aaaagtaaat tactttggca ttgcatttat cagctaaaga
    46681 ttttactatc tcatcatcat agtttaatac acagaaatcc tcaacagttt ggttcataaa
    46741 tatatttgcc tttgcttcta tgtatttttc catagtatgg tgtctattta aatggtcctc
    46801 tgtaatattt aatattgcgc ttactttagg tctaaattca tctatactct caagctgaaa
    46861 actgcttagt tcagttacta acacagactc ttcacttgaa gtttctatag tatctattac
    46921 aggatttcct atattaccaa ctacatatgt atctcgtttt gctcttgaaa aaatttctcc
    46981 taccaaacta gttgtagttg tctttccatt tgtacctgta atccctataa aaattggatt
    47041 attcgatagt ttaaaagcta attctacctc accaattata tacttgcctg aattttttaa
    47101 tttcaatatg aatggtaggt ctagtggtac acctggagac actacaacca tatctatatc
    47161 atctacatct tctgggtgat gtcctaatat gtattttatg tcatttatag atttcaattc
    47221 atcaagtata tttctcaatt tattttcatc ttttatatca tttacgatta cacttgcacc
    47281 caatttatct aaatgtttaa ttgtagatat accagttttt gcaagtccta ctaataaaac
    47341 tttttttcct tttaagtcca tttttcattc cccctagccc taaaatactg ctattatact
    47401 tatccaagct aaaacaactg ttataatcca aaaaataaat acaactcttg tttctggcca
    47461 accacactgt tcaaagtggt ggtgtattgg agccatctta aagaatctct ttcttgttgc
    47521 cttaaaatat ccaacttgaa tcaaaactga tagagcctcc gcaaaataaa ttcctccaat
    47581 tataggtatt ataagcactg agttagttag aactgaaaat gctacaactg cacctcctaa
    47641 tgccattgaa cctgtgtcgc ccataaacac tctagccgga tatgaattaa atcctaagaa
    47701 acctaaacat gctccaacag ttgctgctgc taaaactgcg acttctgtat ttccagcaat
    47761 agaacttgca aatagcataa agaatacaga aactattaat gttactcctg atgctaatcc
    47821 atctaatcca tcagtcaagt ttacagcatt tactattgct acgattataa acatcattat
    47881 tggtatatat aatattccaa catttatcac aaaatctgta aatggtatca taagttgtga
    47941 agcacttgat gatgatgtat actgataaaa agcaacataa aaagataatg caacttgaag
    48001 gattattttt tgatatgctt ttagtccaag tgatcttttt agctttattt ttataaaatc
    48061 atctaaaaag ccaataaaac caaaacctgc aatacatata agacctactg ccatatcatg
    48121 actaactttt actcttgtaa gtccagttat tagaattgca acaatcatta taattccacc
    48181 catagtaggt gttccgtttt tagctagatg tgtttggggt ccatcatctc taactgtctg
    48241 accaaattta aattttctaa gcattggtat aaatattggt cctattataa ttactattaa
    48301 aaacgcaatc aatgcggtgt acgtaagctc tgttattcct aacatcataa aactcctctc
    48361 ccttgtcaat taactcatta gtttaagctt atttatttag atattcgact attttttcta
    48421 atctcatgcc tcttgagcct tttactaaaa tagtgtctcc tgtttttact aattcattta
    48481 acttgttaaa cacatcttct ctattctcaa agtgatatat attggctgaa ttgaatccta
    48541 attgttttgc ttcttctcca ataaacactg aattttcacc tatagttatt ataatatcag
    48601 tattattcat agatgatttt ccaactaatc tatgaccata ctctgaaatt tcacccattt
    48661 ctagtatatc tccaagtatc gctacccttc tacctttata tcttccaagt atctttaaag
    48721 cagcatccat agaatctgga ctagcattat atgaatcatt aattattgtt aatttttcat
    48781 tttttattat gtctaatctc atttttgtcc ctttaaaatt ttttaaacct ttttttatat
    48841 aatctaaaga aatatttaga cacaaaccaa ctaatatagc agacattgca ttatatatat
    48901 tatgttctcc tattgttggt ataaatattt cctctttttt cccatttatt acacatataa
    48961 atgttaaact atcctcttcc atagtataac tttcacaata aacatcattg tttttattaa
    49021 agccaaatgt ttttaactta tatacatgtt ctttttcttt taatgtagat aaaaacttat
    49081 catctccatt tacaattaaa gtatttgttt catcaaagtt tgaagtaatc tccatctttg
    49141 ctttcaaaat accctcttgt gaacctagtt tttctacatg tgataatcct atattcgata
    49201 tgactgcaat tttaggattt actatatcca ctaaatattt tatttcattg aatccagaca
    49261 tacccatctc tattacagca cactcatgtt ctttatttag attaaataaa gtcaatggaa
    49321 ctccaaaatg attatttaag tttttttcat tttttagtat atttaacttt gcagatatgg
    49381 cagagtaaat catatctcta gtagtagttt ttcccacact acctgtaaca cctataaatg
    49441 gaatatcaaa tttttcttta tagtattttg aaatatctcc tagagccaaa gatgtatctt
    49501 ttacctctat taaatttata tccgaacttt ctaattttat tccattactt tcatttttta
    49561 taaaagtttt acatccacta tcatatgcag acttcataaa tgtatgccca tctaaatttt
    49621 ctccaactat ggctacaaat gcattttggg catttgcctc cctgctatct ataactatat
    49681 cacttacaca gtcattataa tctccatata ttaacttccc attagttgca agtactaact
    49741 cttttattgt taagcactcc attattaaac tcctccttta aaaccttagc tatattctct
    49801 tagcaacaaa aggcaatgta taccgttata tatacattgc ctacgataac tcttaacttt
    49861 ataattatat actatatttc tgtttttttg aagatgttta tgggtatatg ttaattatct
    49921 gttacttttt agttttcaaa atatagggtt atacttgagt cttcattaac ttttactcca
    49981 ggttttggga atatatcctt tacctttgta tcttctggaa gttctatatc tgcatctaag
    50041 tctggtttta acttcgcatc ctccaatgcc tttacagcat ctcctatttt tagatttctt
    50101 acatctggca ctttaacttg cttcttttca tactcagctt tttcttcttc tttatatact
    50161 ggtttaactc ccaaatattt taaagagtca ttcattattt ctttcactat tggtcctgct
    50221 gtagtactac caaatgcact tactcctgtt ggttcgtcaa caatagcaag aactactatt
    50281 tgcgggtcat cacaaggtgc tattccaaca aatgaacata tatattttcc tggagcatac
    50341 ttaccatcta taactttttg agctgtacct gttttaccac caagtctata acctggtata
    50401 tatgctatct ttcctccacc ttcagttaca actgattctg caatttctaa cattttttta
    50461 gatgtttctt tagatataac acttcttact ttttttggtt ttactgtttc agttatattt
    50521 cctttattgt cagtataaga ttttaccact cttggttgca ttaaatctcc accattagct
    50581 attgatgata tagctgttat aagttgtatt ggagttactg atatagattg accaaatgat
    50641 attgtagcca attcaactgg tcctacattt ttttcgttat acagtattcc cttagcttct
    50701 cctggtaaat ctatacctgt cttatccata agaccaaatg attctatata atcatacatt
    50761 tttccaacac ctaatctgct tcccaactca acaaatactg ggttgcaaga gttttgtact
    50821 gcttgtttaa attcttgtgt tccatgaggt ctgtaatgtc tccaacattt aatctttctt
    50881 cctccaacag ttacactacc tgtacatgtg aatttttctc catctttaat tactccttct
    50941 tcaagagcac tggaagatgt aataagctta aatgtagaac ctggttcata tgtatcactt
    51001 actgctggat ttctccacat ttgataatag ccttttattt tatctttatc attatatttt
    51061 tcaagttctt cttgataata tggatatatt ggcgttcttg agtcattagg gtcataatct
    51121 ggttttgatg ctaaagcaag tatatcacct gttttagggt tcatagctat tgcagtaacc
    51181 ttttttgcat tatttaactc ataagctttc tgtactgctt tttcagtata gtgttgaatt
    51241 acttcatcta tagacaacac aagaccatta ccttgtacag gctcataata tttttccatt
    51301 ccttgtggta tttctcttcc tgaagcatca gtacttacta taagttttcc aggctttcct
    51361 tttaactttt tatcatattg catttctaca ccagatatac ctgttgcatc tgaagaagta
    51421 tgtcctaaca catatggtgc aaaattccca tatgggtaat atctttgatt atcttctgcc
    51481 acccatattc cacttaattt agcatctctt atttgacttg ccttatcatc atctatccat
    51541 ctttttactt ttacaagagc catatttttt ttgcttatta aagcgtaaat atctttataa
    51601 tcttcatcca aaatctcagc taccttttcg gctgcttctt ttttatcttc tacttccaca
    51661 ggtttacacc aaacagtata cttagttaca cttactgcta actctttcat atttctatca
    51721 tatatagttc ctcttttggg ttctattggt atatctcttg tttgttgttc tagtgctttt
    51781 gtactcaacc aatttccctt cataagttgt agatacccag tcctaataac aaggcaaaaa
    51841 aacaatgcac atgctagaat aagaacgagt accagccttt tcttacttat cctctttact
    51901 tttctcaaat ctattccccc tagttatcca catctatata ctttatttgt ccatcttttg
    51961 gatagtccat attgagcaat tccttagcct ttagctcaat ttcttttatt gatgtttttt
    52021 cagtttcttt tgcctttagt tcctctaata ctatctcttt ttttcttaaa tctttttgta
    52081 aataatagat atcgtacttc atttccgaga tttttgaata accacataga ttcaatatta
    52141 ttactgaaaa agttgcaatc agtaaaatgc atttagctcc tatttttttc ttatttctct
    52201 ttttataatt attctttttt ttatttttat attcatttag attatgtatt ttataatttt
    52261 ttttcaaatt ctcaccccga tagtatatag tttaaatttt cctctatact atatttgtta
    52321 ttaatcatat ttcttcctct aaaatttcat cgctaaatta atacactatt acacaatcta
    52381 attgtatttt tttctataca caacaaaaac ctataaattc acttatactt ttatatgaat
    52441 ctataggtta gatattaatt tctatttatt tttatcactt ctagtatttt tagataattt
    52501 cttccataaa tataatatgt aactaactat ctactctctg attcctaagt tctccattat
    52561 attacttaat accatctata tctatatttt tatatctaaa ttttcattat atatctcaat
    52621 aacaaagaac atttcttata gtgttaattg ttttttaatt gctttaccag tgcaacaaaa
    52681 tatataatac tttttaatta attataataa tcttatttta tatataagat tattctttag
    52741 ccttataact acttatattt tttctgctac tcttagctta gcacttcttg aacgtggatt
    52801 tacttctatt tcttcctcac taggtaatat aggttttcta gttattattt tcacctcaga
    52861 ttctttatca cattggcata taggtaaatg ttgtggacat atacaatctg atgctaaatg
    52921 cttaaatgca tttttcacta ttctatcttc taatgaatgg aatgttatta tacatattct
    52981 tccaccttca ttcattatag aagaagcatc atttatcatc tttgtaatta ctccaagttc
    53041 attatttact tctattcgta ttgcctgaaa agttctttta gctgggtgag gtccatctat
    53101 tctagctttc ttagggatag cttttttaat tacatctact aattctcctg tagtttctat
    53161 tggtttgttt gctctttctt caactataaa tttagctata cgcttagccc agttatcttc
    53221 tccataatct tttattatct tagttaattc atcttctgta taagtattta caacatcata
    53281 tgcagaaaac tcacacctaa catccattct catatcaagt ggtgcatctt gcatataaga
    53341 aaatcctcta tcagcttcat caagttggtg agatgaaact cctaaatcag ctaatacacc
    53401 atcaattttg taaactccta ttttttctaa ttcctctttt atattttcaa aattgctgtg
    53461 aacaaacttc actctacttt catattcact caatctctct tttgctgtgc ttatagcatt
    53521 tttatcttgg tcaaacccta taaataatcc cttatctgac agttttttta ctatttcttt
    53581 agaatgtcct gctcctccca tagtacagtc aacatacacg ccatctggct ttatatttaa
    53641 attctctata cactcattta aaagaactga tacatgatga aattccatag ttacctccct
    53701 taaattcact ttttaaacta ttcttcacta atattatttt tatttttctt tgataaataa
    53761 acatgtgcaa ttatgccacc aataactcca attagactta taacttgtgc cattctaagt
    53821 ggtcctaaca ttaaactatc tgttcttaat ccctctataa aaaatcttcc tattgaatat
    53881 aaagttatat aagtaactat aacttgacct tcatattttt tattttttct aaatagtaat
    53941 aaaactataa atattccaaa atcccatata gactcatata aaaaagttgg atgtaccttt
    54001 actccatcaa ccattattcc ccaaggtaaa ttggtagcac ctccatgtgc ttctccatta
    54061 ataaaatttc cccatcttcc aatagcttgt gctaaaggca ttccaagtat aactgtatca
    54121 gccattttta agaagttaat atttttaatc ttagtgtata tataaccagc taatattcca
    54181 ccaattaacc ctccatgtat agcaagtcca ccacctctaa aattaaaaat ttgagacata
    54241 ttttgagcat agtaactcca attaaataca acataataga ttcttgcaca gattagtcca
    54301 actggaattg ctattatagc tatatttaat acatcatctt ctttaattcc cactcttttt
    54361 gcttctttta ttgctattaa agttcctaaa atcattccac atgccataag tattccatac
    54421 cacatgatgt ctatgccaaa cagtgtaaat gctactctat ccataacgtc acctctttgt
    54481 attaattgaa gatatttcca aagcaatatg ctataattac aattaattgt aattatacta
    54541 aattatgata acacattaat gttaattcta caaaaaatat taaaatttat tctacaactc
    54601 cctacactat attatatcta attactataa aattttttaa actgaatctc tcatgactaa
    54661 agttacaagt gtacatagat attttttaat caaaaagatt gatgcatata tacatcaacc
    54721 tcttctaatt cttatctata gctcttaaat ttttacctca atttatataa atatatttta
    54781 taaatttgtt ttaccaaaat aaacatttaa aaacataaac taatacttaa ttagttattc
    54841 ttattatgat taagtttcta tttaggctct acaatagaaa taacacaata ctcttcttta
    54901 aaatgctctt ttaatctttc ttcaacttcc tcaaaagtaa tatctttaat aacatctaaa
    54961 tactccaata gatttatatc cttaaaaaca taagatataa aactattacc tataaagttt
    55021 atagaatcaa agcacttaat aaatgaccct atttttttct ttttagttct ctcaaactct
    55081 tcttttgata atccttcttt ttttgatttt tctatataat caagaattat ttcttttact
    55141 tttttaggct ctttagaatc tccagctatt atgctaaaag cataatccac ttgagacgaa
    55201 aatccagctc caaaattttc atttattaaa ccttgcatat acaaatcttc ataaagctta
    55261 cttccttttt taaataacat ccctacaagt atatcagtta caatctcttt acgtaaaagt
    55321 tctttccctt ttaaaccaac attactatct ttaaaaccta tgttgaacat aggcatagat
    55381 atagggaatt tttctataac ctctttttct tttacacctt ctggttcttc tggataaaat
    55441 ctttctatac tcttagagag tttatctact ttgtaattgt ttgacttttt agttacatct
    55501 ataacttttt ctacatctaa gtcaccaaca acaaataaag ccatatttcc tggattatag
    55561 aaagtattat aacatttata tagctcttcc ttagttatct tatatatact atcaacagta
    55621 cctgctatat caattctagc aggataattt acatacattg ccttcaagca attaaagtat
    55681 acattccaat cagggtcatc attgtacatt tttatctctt gtgctattat acctttttcc
    55741 ttttcaacat tttcatctgt aaaataaggt gtttgaacat agtcaattag atgttctaaa
    55801 ctctcataaa aattttcagt agcagaaaat aagtaggctg tcattgtaaa attagtaaat
    55861 gcatttgcat ttactcctaa ttttgaaaat ttatcaaatg catctcctcc atcaggttgc
    55921 tcaaacatct tatgctctag aaaatgtgca ataccttcat tcactctaat tttcttatct
    55981 tctccaattg gtacaaattc taagtcattt gaaccataat tagtagctaa aatagcatac
    56041 tttttcataa aacccctttt aggcataaaa tatacatcta atccattttg caatttctca
    56101 taataaactt cttctttcaa tatatcatta actatttttt ccatagacat ctcctccatt
    56161 aatttctcaa gaagtataca gtatcaagct ctatattttt tatagcttca actatatctt
    56221 tttttgtaac cttttctata ctagatatta tatcttgtac ttcagaattt gtctttgcca
    56281 tagattgaga gaaaacaaaa tcagacatac cacctatatt atcagttata gatttcatag
    56341 aattaactaa agcaagttta ctgttttcta attcttcatc agatatatca cctgctttta
    56401 cactttctac ttgttttttt atcaattcta cagctttatc ataatcttta gtttctatac
    56461 cagatgatat aaacatgata cttttatact tctctattga agagaatata tagtaacata
    56521 aactttcttt ttctcttaca ttaacaaata atttagagtg tggaccacca cctaagacat
    56581 tgctcccaac tactaaagca taatatttat ctatgtctgc ataatccaca tttgctctat
    56641 aacccataac taacttacct tgagttattt ccatttgttc atctataact tttacctcat
    56701 ccacattttt tataaaatct gctcttggta tatttataat ttcttctctt tcaaatttta
    56761 gatttttact tattatatct acaactttat cttcatcaaa attaccttct acaactatat
    56821 ctataggtga tgttttaagt atgtttttat aatgttcata taactctctt gaagttatac
    56881 tatctatttc atcttcataa ccaaattcac ttacactata tctctctcct ttacacatag
    56941 cctcaataca tttatcttgc gcatattcct ttttatcatt tattattgat tgtatctttt
    57001 ctcttaaatt ctctttttca atatttaaat attcttcttt aaacccacca tctacaacaa
    57061 gcgtattatt tattacttca ttaaaaaatt ctactacttc ttcaaatata ctctcatcta
    57121 aatacttttc acttatgtta attactttaa aactcaatac ttgacgttct ccacgtttta
    57181 cagcatcagc tcccatagat gaaccgtata aatcatctaa tttattggat attgctctag
    57241 cactaggata tttagagctt ccacttgcta ttattgatgg tattagagca ttttttgtag
    57301 tttctttttc atccaatagt ctctgaacat ataaacttat aagattcgat ttaaactttt
    57361 ctgtttttat caaagtaaga ttaatattat tacctaaatt tattattttt ttagtattaa
    57421 ccatatgtac ctcctatttt tatttaaaca tcaattttaa aattgaaatt tatttatttt
    57481 ttaattacat atactcacat ataaaaacct tctatttaat tatatccttt ttattatttt
    57541 ctaaaagcaa tatttttatt taaattaaat acaaatatta tttaaatcaa tatgctttaa
    57601 agtctagttt acaagcttga tatatatgat gtataatcaa tatgtatgtc tataattaaa
    57661 acaattttgc aatcctatag attgattgtt taaagttata taaagaatat aaaaaagaat
    57721 aagagagagg acgattaata tgaaattagg tatagttggt ttaccaaatg taggtaaaag
    57781 cacactattt aacgcaataa ctcaagcagg tgcagaatct gcaaattacc ctttttgtac
    57841 aatagaccca aatgtgggag ttgtatctgt tccagatgaa agattaaaca aactacaaga
    57901 attatataat tctgaaaaaa tagttcctac tgctatagaa ttttgtgata tagcaggttt
    57961 agttagagga gcttctaaag gagaaggttt aggaaataaa ttcttatcac atataagaga
    58021 agttgatgca atagttcatg ttgttagatg ttttgaggat gaaaatgttg tacatgtaga
    58081 tggttctgta gacccacttc gtgatataga aactataaac ttagaattaa ttttctcaga
    58141 tatagagatt cttgaaagaa gaattaataa aactcaaaaa gctgctaagg ctgataaaac
    58201 tttaggttct gaacttgatt tattaaaatc aataatgtct actttagaag aaagtaagtg
    58261 tgtaagaact atggaattta cagaagatga acaaacattc gtaaattcat tggatttact
    58321 tacatctaag cctgtaatat atgcttcaaa tgtatcagaa gatgatttag ctgataatgg
    58381 agaaaataat aaatatgttc aacaagttaa agcatttgca gaaacagaag atgccgaagt
    58441 tgttgtagta tgtgcacaaa tagaagcaga aatttctgaa cttgattctg ctgaagaaaa
    58501 gaaagagttt ttagaaacat taggtcttga acaatcaggg cttgataagc ttataaaatc
    58561 ttcttatgct ttacttggtt taatttcatt cttaactgct ggtccaaaag aagttagagc
    58621 atggactata aaagttggaa gtaaagctcc tcaggcagga ggaaaaatac attctgatat
    58681 agaaagagga tttatcagag cagaaacaat agcctttaat gatttggttg agcatggtac
    58741 tatggctgct gctaaagaaa aagggttggt taaattagaa ggtaaagaat acatagttaa
    58801 agatggagat gtaatattat ttagatttaa tgtttaaaat gattaatcgt atacactttg
    58861 actattatat ataataacta aaaaactaag attataataa tac
//
LOCUS       DBGJFU010000010        56168 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.010, whole genome shotgun sequence.
ACCESSION   DBGJFU010000010 DBGJFU010000000
VERSION     DBGJFU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.010"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="ACOIAS_000602"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="ACOIAS_000602"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            179..253
                     /locus_tag="ACOIAS_000603"
     tRNA            179..253
                     /locus_tag="ACOIAS_000603"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Asn,seq:gtt)
     gene            259..344
                     /locus_tag="ACOIAS_000604"
     tRNA            259..344
                     /locus_tag="ACOIAS_000604"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Leu,seq:taa)
     gene            360..435
                     /locus_tag="ACOIAS_000605"
     tRNA            360..435
                     /locus_tag="ACOIAS_000605"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Met,seq:cat)
     gene            443..517
                     /locus_tag="ACOIAS_000606"
     tRNA            443..517
                     /locus_tag="ACOIAS_000606"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
     gene            532..605
                     /locus_tag="ACOIAS_000607"
     tRNA            532..605
                     /locus_tag="ACOIAS_000607"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:tcc)
     gene            611..686
                     /locus_tag="ACOIAS_000608"
     tRNA            611..686
                     /locus_tag="ACOIAS_000608"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:644..646,aa:Val,seq:tac)
     gene            692..768
                     /locus_tag="ACOIAS_000609"
     tRNA            692..768
                     /locus_tag="ACOIAS_000609"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Asp,seq:gtc)
     gene            779..853
                     /locus_tag="ACOIAS_000610"
     tRNA            779..853
                     /locus_tag="ACOIAS_000610"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Gly,seq:gcc)
     gene            863..936
                     /locus_tag="ACOIAS_000611"
     tRNA            863..936
                     /locus_tag="ACOIAS_000611"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Arg,seq:tct)
     gene            1920..2759
                     /gene="cdaA"
                     /locus_tag="ACOIAS_000612"
     CDS             1920..2759
                     /gene="cdaA"
                     /locus_tag="ACOIAS_000612"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HGZ2700333.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2756..3937
                     /locus_tag="ACOIAS_000613"
     CDS             2756..3937
                     /locus_tag="ACOIAS_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HGZ2700334.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4241..5143
                     /gene="ptb"
                     /locus_tag="ACOIAS_000614"
     CDS             4241..5143
                     /gene="ptb"
                     /locus_tag="ACOIAS_000614"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HGZ2700335.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5165..6244
                     /gene="buk"
                     /locus_tag="ACOIAS_000615"
     CDS             5165..6244
                     /gene="buk"
                     /locus_tag="ACOIAS_000615"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HGZ2700336.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6234..6911
                     /locus_tag="ACOIAS_000616"
     CDS             6234..6911
                     /locus_tag="ACOIAS_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2700337.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6954..7169
                     /locus_tag="ACOIAS_000617"
     CDS             6954..7169
                     /locus_tag="ACOIAS_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2700338.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7198..8277
                     /locus_tag="ACOIAS_000618"
     CDS             7198..8277
                     /locus_tag="ACOIAS_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HGZ2700339.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8277..9029
                     /locus_tag="ACOIAS_000619"
     CDS             8277..9029
                     /locus_tag="ACOIAS_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HGZ2700340.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            9030..9587
                     /locus_tag="ACOIAS_000620"
     CDS             9030..9587
                     /locus_tag="ACOIAS_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HGZ2700341.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9757..11103
                     /gene="glmM"
                     /locus_tag="ACOIAS_000621"
     CDS             9757..11103
                     /gene="glmM"
                     /locus_tag="ACOIAS_000621"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGZ2700342.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11370..13202
                     /gene="glmS"
                     /locus_tag="ACOIAS_000622"
     CDS             11370..13202
                     /gene="glmS"
                     /locus_tag="ACOIAS_000622"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGZ2700343.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13653..14948
                     /locus_tag="ACOIAS_000623"
     CDS             13653..14948
                     /locus_tag="ACOIAS_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGZ2700344.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLIKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNRQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            15398..16135
                     /locus_tag="ACOIAS_000624"
     CDS             15398..16135
                     /locus_tag="ACOIAS_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HGZ2700345.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTKYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            16156..17409
                     /gene="murA"
                     /locus_tag="ACOIAS_000625"
     CDS             16156..17409
                     /gene="murA"
                     /locus_tag="ACOIAS_000625"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGZ2700346.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            17496..18560
                     /gene="spoIID"
                     /locus_tag="ACOIAS_000626"
     CDS             17496..18560
                     /gene="spoIID"
                     /locus_tag="ACOIAS_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HGZ2700347.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            18626..19294
                     /locus_tag="ACOIAS_000627"
     CDS             18626..19294
                     /locus_tag="ACOIAS_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HGZ2700348.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            19392..19652
                     /gene="spoIIID"
                     /locus_tag="ACOIAS_000628"
     CDS             19392..19652
                     /gene="spoIIID"
                     /locus_tag="ACOIAS_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HGZ2700349.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            19849..20868
                     /locus_tag="ACOIAS_000629"
     CDS             19849..20868
                     /locus_tag="ACOIAS_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HGZ2700350.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDNRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            20884..21312
                     /gene="fabZ"
                     /locus_tag="ACOIAS_000630"
     CDS             20884..21312
                     /gene="fabZ"
                     /locus_tag="ACOIAS_000630"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGZ2700351.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRRNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(21423..21932)
                     /gene="yyaC"
                     /locus_tag="ACOIAS_000631"
     CDS             complement(21423..21932)
                     /gene="yyaC"
                     /locus_tag="ACOIAS_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HGZ2700352.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      22121..22224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22360..23553
                     /gene="metK"
                     /locus_tag="ACOIAS_000632"
     CDS             22360..23553
                     /gene="metK"
                     /locus_tag="ACOIAS_000632"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGZ2700353.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            23868..24965
                     /locus_tag="ACOIAS_000633"
     CDS             23868..24965
                     /locus_tag="ACOIAS_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700354.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMTIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            25229..27448
                     /locus_tag="ACOIAS_000634"
     CDS             25229..27448
                     /locus_tag="ACOIAS_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HGZ2700355.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHVEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            27493..28233
                     /locus_tag="ACOIAS_000635"
     CDS             27493..28233
                     /locus_tag="ACOIAS_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HGZ2700356.1"
                     /translation="MINKCLDFIYPENISCIICDKSIKKTNTYSICKSCFKEMNFIQD
                     GCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDVTKKIVLGLKYNQKT
                     FMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQKIAFNLSKIVNIPLL
                     DCISRKKYTRMLYKLNKKERKEELKNVFVVKDNVKLINNKNILLIDDIFTTGLTTNEI
                     SKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            28565..30478
                     /locus_tag="ACOIAS_000636"
     CDS             28565..30478
                     /locus_tag="ACOIAS_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2700357.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIGGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMFTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSSNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIRIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            30619..30927
                     /locus_tag="ACOIAS_000637"
     CDS             30619..30927
                     /locus_tag="ACOIAS_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2700358.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            30951..31277
                     /locus_tag="ACOIAS_000638"
     CDS             30951..31277
                     /locus_tag="ACOIAS_000638"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2700359.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            31316..32650
                     /gene="celB"
                     /locus_tag="ACOIAS_000639"
     CDS             31316..32650
                     /gene="celB"
                     /locus_tag="ACOIAS_000639"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HGZ2700360.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGSDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQVVLLILGIFMYIPFIKMLDKQYLREENATVEEEDDDISFDDLVL"
     gene            32661..33179
                     /locus_tag="ACOIAS_000640"
     CDS             32661..33179
                     /locus_tag="ACOIAS_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdB-related putative oxidoreductase"
                     /protein_id="HGZ2700361.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGIMMEQFLNE
                     SKVIATLFCGDEFFVNNEEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            33275..34234
                     /locus_tag="ACOIAS_000641"
     CDS             33275..34234
                     /locus_tag="ACOIAS_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HGZ2700362.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            34283..35335
                     /locus_tag="ACOIAS_000642"
     CDS             34283..35335
                     /locus_tag="ACOIAS_000642"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HGZ2700363.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            35338..36012
                     /locus_tag="ACOIAS_000643"
     CDS             35338..36012
                     /locus_tag="ACOIAS_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGZ2700364.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            36565..37116
                     /gene="raiA"
                     /locus_tag="ACOIAS_000644"
     CDS             36565..37116
                     /gene="raiA"
                     /locus_tag="ACOIAS_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGZ2700365.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            37248..39923
                     /gene="secA"
                     /locus_tag="ACOIAS_000645"
     CDS             37248..39923
                     /gene="secA"
                     /locus_tag="ACOIAS_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGZ2700366.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            39938..41045
                     /gene="prfB"
                     /locus_tag="ACOIAS_000646"
     CDS             join(39938..40012,40014..41045)
                     /gene="prfB"
                     /locus_tag="ACOIAS_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGZ2700367.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            41189..43330
                     /locus_tag="ACOIAS_000647"
     CDS             41189..43330
                     /locus_tag="ACOIAS_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGZ2700368.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLEKLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMPPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DNEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKTIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNICEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            43478..44644
                     /locus_tag="ACOIAS_000648"
     CDS             43478..44644
                     /locus_tag="ACOIAS_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HGZ2700369.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      44704..44821
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            44945..45550
                     /locus_tag="ACOIAS_000649"
     CDS             44945..45550
                     /locus_tag="ACOIAS_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGZ2700370.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPFVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVILSLVTLPLYKKMGNVIKK"
     gene            45879..46919
                     /locus_tag="ACOIAS_000650"
     CDS             45879..46919
                     /locus_tag="ACOIAS_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HGZ2700371.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTERLIIKVIRHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVENQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            47058..47510
                     /gene="tsaE"
                     /locus_tag="ACOIAS_000651"
     CDS             47058..47510
                     /gene="tsaE"
                     /locus_tag="ACOIAS_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGZ2700372.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDDGREMILTPKGEFYKEIVEELIK"
     gene            47510..48226
                     /gene="tsaB"
                     /locus_tag="ACOIAS_000652"
     CDS             47510..48226
                     /gene="tsaB"
                     /locus_tag="ACOIAS_000652"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGZ2700373.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            48216..48692
                     /gene="rimI"
                     /locus_tag="ACOIAS_000653"
     CDS             48216..48692
                     /gene="rimI"
                     /locus_tag="ACOIAS_000653"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGZ2700374.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            48698..49714
                     /gene="tsaD"
                     /locus_tag="ACOIAS_000654"
     CDS             48698..49714
                     /gene="tsaD"
                     /locus_tag="ACOIAS_000654"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGZ2700375.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKEKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            50011..52719
                     /gene="hpdB"
                     /locus_tag="ACOIAS_000655"
     CDS             50011..52719
                     /gene="hpdB"
                     /locus_tag="ACOIAS_000655"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HGZ2700376.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKSAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATADGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            52723..52980
                     /gene="hpdC"
                     /locus_tag="ACOIAS_000656"
     CDS             52723..52980
                     /gene="hpdC"
                     /locus_tag="ACOIAS_000656"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HGZ2700377.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            52973..53923
                     /gene="hpdA"
                     /locus_tag="ACOIAS_000657"
     CDS             52973..53923
                     /gene="hpdA"
                     /locus_tag="ACOIAS_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HGZ2700378.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLGELQDIFLDNGIACYVGHETAF"
     gene            54238..54708
                     /locus_tag="ACOIAS_000658"
     CDS             54238..54708
                     /locus_tag="ACOIAS_000658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700379.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYIFQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVS"
     gene            54721..55200
                     /locus_tag="ACOIAS_000659"
     CDS             54721..55200
                     /locus_tag="ACOIAS_000659"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGZ2700380.1"
                     /translation="MLVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNP
                     DVTFLSVINIIIIGILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGII
                     TTQITHGNLLNGGMFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(55299..>56168)
                     /locus_tag="ACOIAS_000660"
     CDS             complement(55299..>56168)
                     /locus_tag="ACOIAS_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGZ2700381.1"
                     /translation="TYSLGLLLYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNND
                     MYEFYSLIILFFLTYSLLIVLKLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLY
                     PIKNIKLGSLLIIYFMISVIVPTSFILILQGSQPNYSGIIKFIFTPITSLTTIYIFFS
                     EEYAWRGFLQNIFFNKFGKKLGVIILGICWSLWHLPLIFTLYTPETPILGLILRSIHI
                     VGISIFLGYLYIKTKNIWFCAIIHILNNSAFLITNSKESTITYHDILIVSMVVLIFYV
                     PFLFTKEYKNNLE"
BASE COUNT        22499 a         6085 c        10664 g        16920 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc atttcgaaca cagaagttaa gctccctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg ttaaatgttc
      181 caaggtagct caacggtgga gcaaccggct gttaaccggt aggctgtggg ttcgagcccc
      241 acccttggag ccaaaaaagc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      301 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcacttata aaacaatatc
      361 gcggggtgga gcagttggca gctcgtcggg ctcataaccc gaaggtcgta ggttcaagtc
      421 ctgcctccgc aaccattaaa acggctcgtt ggtcaagagg ttaagacacc gccctttcac
      481 ggcggtaaca ggggttcaat tcccctacga gtcaccaatt caattaaata tgcgggtgta
      541 gctcaatggt agagttctgg ccttccaagc cagctgtgag ggttcgatcc ccttcacccg
      601 ctccaaaaat gggaccatag ctcagctggg agagcacctg ccttacaagc agggggtcac
      661 aggttcgagc cctgttggtc ccaccattcg cggcctggta gttcagttgg ttagaatgcc
      721 agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa ttaaataagc
      781 gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc
      841 gtttcccgct ccataataac ttgggtgcat agctcagctg gatagagcaa cgcccttcta
      901 aggcgtgtgt ccggggttcg aatccctgtg cgctcattaa gaaaagttta ctataaatta
      961 tagtaaactt ttatattgtt taaaaagaca caggaaacat gtatattacc tgtgtctttt
     1021 tatttataaa ctttaaacta tgtaaaaatt acagtatatt ctaatagagt tacagtatat
     1081 tcattaatat tgtaaacata cggagggtaa tgtataatag tctacaagat gtttatgaag
     1141 gcatcagtta aatataataa atataactaa ttagtaagag ttatatacat tatattgtat
     1201 actttatggt atttatatat tgaaataact tatgaaatta tttataaaga gattcatgag
     1261 catatttttg tctaaacaat aagtataaga aattataaaa agttagagac tatagtgaat
     1321 ttacaattta atgtaatatt tataagttat actaaataaa ttattatact gtgaaatgga
     1381 tatataactt taggtattgt gtattatttt cactgtagga gattatatct agttatacat
     1441 aataagtttt aatgatagtt tatagtaaga tattaaaata aatttatcta atctagatat
     1501 gaaataagtt attactttaa tataaaaatg aatctaatgg aataatattg atgataagta
     1561 atatttagat tttgtatttg tttatagtta ataagtggat tatagaattt ataataatta
     1621 atatatatta aacaaagata tattaagata ttttagaaca taaaactgaa ttaaggcgcg
     1681 agtactattt tgtattcgcg ccttgatggt ttgagttatt tgagtaaaaa ctaactttaa
     1741 taagaaatat gtatttatta agttgatatt tttaatgtgt ttagagatta tttaacaatc
     1801 tccaaatata aaactagaat tatttacata aaagttaaat agtttgtata gagttagatt
     1861 tatatagtat aatacttagt gtaaataatt aagaagttat caaacaggag gataaaactg
     1921 tgttagatat aaattctttt ttaaatggat tttttaatat aatattaaga atgagtatat
     1981 atgatttaat tgacatatct atagtagcgt atatttttta caaaatattt atgtttataa
     2041 aagatacaag agcagaacaa gtgtttaaag gtataatatt tcttttatta gcaactcaac
     2101 ttagtaacac ctttaaattg cataccgttt attggatatc tcttaaagct ttagattatg
     2161 gagttatagc tgctcttata atttttcaac cagaatttag agcaggactt gaacatatag
     2221 gaagagcaaa gtttaattta tttggaaaga atgtaaatac ttctgaagaa actctcaata
     2281 gaaatataga agaaattgta gaagctttat attcactttc tagacaaaaa ataggagctc
     2341 ttatcataat ggagagagaa actagaataa gtgatataat aaatacagga actattattg
     2401 atgctgaaat ttctaggcaa ctattaataa atatatttat accaaataca ccattacacg
     2461 atggagctgt tgtaattaga gattctaagg taaaagcagc tgcatgtttt ttgccattga
     2521 cagaaagtaa ggatttaagt aaagatttgg gcactagaca tagagcagga ataggggtaa
     2581 gtgaggtgtc tgactgtata actttgattg tatcagagga aacaggtggc gtatcaattg
     2641 ctaaagcagg taagttatat agagatattt ctagagaaag aatgatgaat atattacgta
     2701 gtaatttaaa aacaaataca gaaacaagaa gttttttcaa aggtggtata tttaaatgat
     2761 aaatagacta aaaaataata ccaaaataaa aataatatca tttttaagtg caattgcatt
     2821 atggatgtat gttatggctg tagttgaccc tgaagaaact aaagggtttg aagatatacc
     2881 tgtatctata agtaatatga gtgatttaaa ggataaaaat cttgttatat atccagacga
     2941 aaaattaaca gcagatgtat atataaaagg tagtttatca aaggtacaaa gtgttaaaaa
     3001 agaagatata catgtatatg gaaccattga agacccaata caaggacaga aggaagtata
     3061 tttaaaagca aatattcctc aaggtgttac tctagaattt aagcatgatg ttttagtagt
     3121 aaatcttgaa aaaaacataa aagaaaaaag agatataaag gtagaagtaa gtggaaattc
     3181 aaaaagaaat ataaataaaa ttgaacttag taaggaattt gtcgatgtat caggtccaag
     3241 aactctagtt aaagaagtca aaagtgtaag agctaatcta gatgttggta atgagacaga
     3301 tgatttttct aaaaaattgc aattaatacc attaaatagt gatggaacta aggttgaagg
     3361 agtaacatta agttcatcct ttgtgactgc aaagatagag ctattaaagg aaaaaattgt
     3421 cccaataaga gtaagtctaa atgataataa agataatgat gtactgaaaa actataaatt
     3481 aagtaaggaa caagttacaa taaaaggaaa gaaagaagat atagacaaaa taagttatat
     3541 taagacaaaa cctattgact tagctgattt ggctaatggt gaagaaaaag aagttagctt
     3601 agaaattcca gagggtataa ctcttgagga tagctctata acaattaaat taaatgatac
     3661 aaaacaattg agtgcagaat ttttatataa tgcgggagat attgaattaa gaaatatccc
     3721 aacaaatatg gataatccaa atgtaatttc tgatgtagat ataaaagttg ttgtagagtc
     3781 aacggaggat ttgagtaagt taaataaaaa agatatttca ttatatattg atttatcaca
     3841 aggtcctgag gaagatagta aatataaaat aaaatatgaa acaacatatc aatttaaaaa
     3901 aataaatata gaacctaata ttgttgaagt ggaatgaaca aatatttcga atatttagtt
     3961 gttacaagat gcgacaaaaa aacctataaa attaagtttg ttaattttat gtttttgttg
     4021 catcttgttt attttagtta tattataaaa taaggacaat gagatatttg ttaaatctaa
     4081 aaagcgctag atatatgaaa aaagactgat aaatagattt aaataaatat tttgaattaa
     4141 atatttcaat tatctctagg aaaacgttgc tgaacgagtt ataatttaat tgagatataa
     4201 aaagttctat ttcaaaaaat tcaagatgag gtggaaatgc atgagaagtt ttgaagaagt
     4261 aattaagttt gcaaaagaaa gaggacctaa aactatatca gtagcatgtt gccaagataa
     4321 agaagtttta atggcagttg aaatggctag aaaagaaaaa atagcaaatg ccattttagt
     4381 aggagatata gaaaagacta aagaaattgc aaaaagcata gacatggata tcgaaaatta
     4441 tgaactgata gatataaaag atttagcaga agcatctcta aaatctgttg aattagtttc
     4501 acaaggaaaa gccgacatgg taatgaaagg cttagtagac acatcaataa tactaaaagc
     4561 agttttaaat aaagaagtag gtcttagaac tggaaatgta ttaagtcacg tagcagtatt
     4621 tgatgtagag ggatatgata gattattttt cgtaactgac gcagctatga acttagctcc
     4681 tgatacaaat actaaaaagc aaatcataga aaatgcttgc acagtagcac attcattaga
     4741 tataagtgaa ccaaaagttg ctgcaatatg cgcaaaagaa aaagtaaatc caaaaatgaa
     4801 agatacagtt gaagctaaag aactagaaga aatgtatgaa agaggagaaa tcaaaggttg
     4861 tatggttggt ggaccttttg caattgataa tgcagtatct ttagaagcag ctaaacataa
     4921 aggtataaat catcctgtag caggaagagc tgatatatta ttagccccag atattgaagg
     4981 tggtaacata ttatataaag ctttggtatt cttctcaaaa tcaaaaaatg caggagttat
     5041 agttggggct aaagcaccaa taatattaac ttctagagca gacagtgaag aaactaaact
     5101 aaactcaata gctttaggcg ttttaatggc agcaaaggca taattttaag ggggaaatag
     5161 gcaaatgagc aaaatattta aaatcttaac aataaatcct ggttcgacat caactaaaat
     5221 agctgtattt gataatgagg atttagtatt tgaaaaaact ttaagacatt cttcagaaga
     5281 aataggaaaa tatgagaagg tgtctgacca atttgaattt cgtaaacaag taatagaaga
     5341 agctctaaaa gaaggtggag taaaaacatc tgaattagat gctgtagtag gtagaggagg
     5401 acttcttaaa cctataaaag gtggtactta ttcagtaagt gctgctatga ttgaagattt
     5461 aaaagtggga gttttaggag aacacgcttc aaacctaggt ggaataatag caaaacaaat
     5521 aggtgaagaa gtaaatgttc cttcatacat agtagaccct gttgttgtag atgaattaga
     5581 agatgttgct agaatttctg gtatgcctga aataagtaga gcaagtgtag tacatgcttt
     5641 aaatcaaaag gcaatagcaa gaagatatgc tagagaaata aacaagaaat atgaagatat
     5701 aaatcttata gttgcacaca tgggtggagg agtttctgtt ggagctcata aaaatggtaa
     5761 aatagtagat gttgcaaacg cattagatgg agaaggacct ttctctccag aaagaagtgg
     5821 tggactacca gtaggtgcat tagtaaaaat gtgctttagt ggaaaatata ctcaagatga
     5881 aattaaaaag aaaataaaag gtaatggcgg attagttgca tacttaaaca ctaatgatgc
     5941 tagagaagtt gaagaaagaa ttgaagctgg tgatgaaaaa gctaaattag tatatgaagc
     6001 tatggcatat caaatctcta aagaaatagg agctagtgct gcagttctta agggagatgt
     6061 aaaagcaata ttattaactg gtggaatcgc atattcaaaa atgtttacag aaatgattgc
     6121 agatagagtt aaatttatag cagatgtaaa agtttatcca ggtgaagatg aaatgattgc
     6181 attagctcaa ggtggactta gagttttaac tggtgaagaa gaggctcaag tttatgataa
     6241 ctaatgataa aagaataaga atcataactg gtcactatgg aagtggtaaa agtgaatttg
     6301 cgatgaacta tgttgttaag ttaagagata tggtttctgg caaagttgct atagccgatt
     6361 tagatgtggt aaatgtatac tttagaacta gagaaaagaa agagttaatg aaatccttag
     6421 gtatacaacc aatagatagc tcaattaatg ctccaacatt agatttacca gcagtatcag
     6481 cagaggtaat gtctcctatg gtagaccatt catacaattc tgttatagat ttaggtggag
     6541 ataatgtagg tgctagggtt ataggaagat ttagtcattt gttaaaagaa ggcgattatg
     6601 atatgctttt tgtaataaat gcaaataggg agaaaactca aacttctgaa gaagttatcc
     6661 aatatataaa agaaatagaa aaatcttcta aattaaaggt aacaggtcta attaataata
     6721 ctcatcttat taggtttact actattgatg atgttttaag aggtcagaaa gttgcaaaag
     6781 aagtttctga aaaatgtaat ataccgataa gatatgttgc atgtttagaa aatcttgttg
     6841 aacagttacc aaaagattta gaaggagaaa tttttcctat aaaattatat atgagagaag
     6901 actggatgta gtttgagctc aaatatatag aaagtttaag gaggcttagg taaatggcta
     6961 aaggaaaagt atcttttaat caagaacgtt gtaaaggttg tggattgtgt gttgaagcat
     7021 gtccagtaaa aatcatacaa ttagactcaa atgtaataaa caaaaaaggg tataacccag
     7081 caactgtttt tgaaatggaa aaatgcatag gatgtgcaag ttgtgcaact atgtgtccag
     7141 atgtagttat aacagtagaa agagattaat ttaattaata tttaaggggg aattttaatg
     7201 gctaagatac ttatgaaagg taacgaagca tttggaaaag cagctataga agctggttgc
     7261 aaatatttct tcggttaccc aataactcca caaagtgaat taccagaata tttatcaaga
     7321 gagttaccta aaataggtgg agcgttcgtt caagctgaat cagaagtttc tgcgataaac
     7381 atggtttatg gtggagcagg tgcaggagca agagttatga cttcttcatc ttcaccagga
     7441 gttgctttaa agcaagaagg tataacatat gcagtaggag cagaagttcc ttgtgttgta
     7501 cttaatgtaa tgagaggtgg tccaggactt ggaagtatac aaccttcaca agctgactac
     7561 tttatgtcta caagaggtgg aggaaatgga gactacagaa ctccagtatt cgcaccagct
     7621 acagttcaag aagctgttga tatgataatg gaagcttttg acgttgcaga ttattacaga
     7681 tcaccagtta tggtagttgc agatggtatg ataggtcaaa tgatggagcc agttgaattt
     7741 agagctcctg agaagaaaag agaattgcct cctaaagatt gggcaactgt tggaactaaa
     7801 ggaaaaagaa aacctaatgt aataaactct ctatacttag agccagaagt actagaagac
     7861 cattgttggc atttacaaga aaaatttgat gcaatggaaa agaatgaagt acaatacgaa
     7921 atgtacaaaa cagaagatgc tgaatttgta tttgcagctt atggaactac ttcaagagta
     7981 gtaaaaagcg caattgatat attaagagaa gaaggaataa aagcaggtct tataagacct
     8041 aaagtattat ggccattccc attcgaagca ttcaaccaaa ttcctaatgc tagaaacata
     8101 ttgactgttg aaatgagtat gggacaaatg gtggaagatg ttaaaatggc agtagaaggt
     8161 aaattaccag tttacttcca tggaagacca ggaggaatga ctccaacacc agcagaaata
     8221 gttgaaaaag ctaaaaaaat tattgccgga gaattggttg caggaggtgc tagataatgg
     8281 ctgttgtatt taagaaaact gaaggattac aagatactca aactcactac tgtccaggat
     8341 gtacacatgg tataatccat agattagttg gtgaagtatt agaagaacta ggagtattag
     8401 gtgatgctgt aggtgttgtt ccagttggat gttcagtttt aggatataaa tattttaact
     8461 gtgacactca agaagctgct catggtagag cgccagctgc tgctacagga ataaaaagag
     8521 ttcatcctga aaatacagta ttcacttatc aaggtgatgg agacttagct tcaattggta
     8581 ctgctgaaat agttcatgca gctgctagag gagaaaaaat aactactata tttgtaaaca
     8641 atacaactta tggtatgact ggtggacaaa tggctccaac tacattagtt ggacaaagag
     8701 ctactactgc tcaatcagga agaaatgctg aaactcaagg ttatccaata agagtaagtg
     8761 aaatgttagc tacattaaca ggagctgtat ttgtagaaag agtagcagtt gatactccag
     8821 ctcatgttag acaagctaaa aaagctataa agaaagcatt ccaagttcaa caagcaggat
     8881 taggatttgg aatagtagaa gtattatcta cttgtcctac aaactgggga ctagctccaa
     8941 acgatgcttt acaatggtta agagacaaca tgataccata ctatccatta ggaaatttca
     9001 aaaatgttga agttgaggag gtgaagtaaa tgtctacagc tagagtaata tgtgcaggct
     9061 tcggaggcca aggtgttatg tctatgggac aattacttac ttatgcagga atgctagaag
     9121 gaaaagaagt ttcttggtta ccatcttatg gaccagaaat gcgtggaggt acagcaaact
     9181 gtgcagtaac tgtatctaat gagccagttg gttctccact tataacagat gatgcaactg
     9241 cagctatatt attaaatata ccagcatttg aaaaattcaa agatgatgtt attcctggtg
     9301 gaaaaataat agtaaatagt tctttaataa aagaaaaagt tgatagaaca gatgtagatg
     9361 tttattatat accagcaaat gaattagcag ctgaattagg aaatgataaa gttgctaaca
     9421 tgataatgtt aggtgcttac ttaaaagtat ctgatactgt agatatagaa tctgtactag
     9481 aagcattcaa aaaagttttc ggacctagaa aagaaaaatt cgttccatta aacagagaag
     9541 ctttacaaaa aggtatggat gctacatgtt ctaaagcaac taactaataa agaaaaagat
     9601 gtcaaaaaaa agacaataga attatttcta ttgtcttttt tgctgataag tattaaaata
     9661 tttttatgac tgaaattaag cctataatga tattttatga actataaaaa tataatacaa
     9721 attacaagct tttcaattat gaaaggagaa tctttagtga gaaagtattt tggaacagat
     9781 ggagttagag gagtagcaaa tacagaacta acatgtgatt tagcatataa acttggtaga
     9841 gctggtggat ttgtcttagc tcaaggagac cacagagtaa aagtagtagt aggtaaggat
     9901 actagaatat caggtgatat gttggaagca tctttaatag caggacttat gtctgttgga
     9961 tgtgatgtta ttactgttgg aataatacca acacctgcag tggcatattt aactagaaaa
    10021 tatggtgctg attgtggtgt ggttatatct gcatctcaca atcctgttga atataatgga
    10081 atcaaattct ttaataagaa tggatataaa ttagatgatg aaattgaatt gaaaatagaa
    10141 gaatatatag acgatataga caaaattgat tgtcttccaa ttggtgaaaa tgtaggaaga
    10201 aaattacatg aacattgtgc acaaagagac tatgtagact acctaaaatc aataataagc
    10261 actgacttta agggattaaa agtagttttg gactgtgcta atggtgcgtc atataaagtg
    10321 gcaccaatag tgtttgatga actaggagca agtgtaattt caataaatag ttcaccagat
    10381 ggaaataata taaactataa atgtggctct acacatccag aacaacttca aagggctgtt
    10441 ttggaacata atgctgactt aggtcttgca tatgatggag atgctgatag attaattgca
    10501 gtaaatgaaa agggtcaaat tgttgatgga gaccatataa tgatattgag tgccttaaat
    10561 ttaaagaaaa ataataaatt ggcacaagat actttggtag ttacagttat gagtaatata
    10621 gggttgacaa tagctgctaa ggaaaatgga ataaatttat ctactacagc agttggagac
    10681 agatatgttt tagaagatat ggttaaaaat ggatataatc ttggtggaga gcaatctgga
    10741 catatgatat ttttagatta caacactact ggagatggag tgcttagttc tctaatactt
    10801 gcaaatataa tcttgcaaga aaagaaacct ttatcagaaa ttgcatcaat aatgagccaa
    10861 tacccacaag tattagtaaa tgcaacaata aaaaatgaaa ataagaacaa atatatggaa
    10921 tatccagaaa taaaaactga gatagaaaga atagaatcta ttttagacgg aaatggtaga
    10981 gttttgataa gaccatcagg aactgagcca ttagttagag ttatgttaga gggtaaagag
    11041 gaaggtcaaa taaaagaatt agcaacaaac ttagctaatc ttatacaaga aaaattatcg
    11101 taatttaaag ggtattctca gatataaata ctgaggacac ctttctaagc gccagaactt
    11161 agttatagac ataattattg agtaatatag gaactaagtt gacgaggtca gagttaatcg
    11221 aattgtcggc ggatgctctg cggtgtgttt accatcgaaa gacttcttta aataatagga
    11281 gcaatcctat ggacaaaggg aagccaaagt aaacttatat gacctaactc ttaaaatata
    11341 taaataatat ttttaggagg acacaaataa tgtgtggaat agttggatat ttaggaagta
    11401 gaaaggcagc agaagttata gtagaagggc tttctaagct agagtacaga ggttatgact
    11461 cagcaggagt agcagttaat agttctaatg aaaaagaact taatattaga aagtttaaag
    11521 gtagactttc tgtacttgca gaagacttag aaaagaatcc tattgatgga aatttaggta
    11581 tagggcatac gagatgggca actcatggag aaccatctga tgtaaactca catcctcatt
    11641 ttaatcaagc caaaacaata gcagttgttc ataatgggat aatagaaaac tatatggaaa
    11701 taaaagaaga attaatttct gaaggtgtta agtttgaatc tcaaacagat acagaggtaa
    11761 tagcacattt agtagataaa tactatgagg gtaatttatt agatgctgta tacaaaacta
    11821 tatcaaaatt aagaggagct tatgcattag gtgttatatg taaagaacat ggaaatgaat
    11881 tagttgcagt aagaaaagat agtcctcttg tagttggtgt tggtgaaggt gaaaacttta
    11941 tagcatcaga tatacctgca ttattaaaat atactagaga tgtttatttc ttagaaaatg
    12001 gagaagtagt tcatctaaaa gacgaaaatg taacagttta tgactctaat agaaatcttg
    12061 ttgaaaaaga agtatttcat gtaacttggg atgtagaagc tgcgtctaaa ggtggatatg
    12121 actactttat gtcaaaagaa attcatgagc aaccaacagg agtaagagaa actttagaaa
    12181 gaagacttga tgataatgga aatataattc tagacagtat aaatatttct aaagaagact
    12241 tagaaaaaat aaataaagtt tatatagtgg cttgtggaac agcatataat gctggattac
    12301 ttggtaagta tgccatagaa aaatttgtaa atataccagt aataactgat atagcatcag
    12361 aatttagata tagcgaccca tttgtagatg aaaattcatt agtaatatta gttagtcaat
    12421 ctggtgagac agcagatact ctagcagtat taagagattc aaaggctaaa ggagcaagaa
    12481 tactttcaat aactaacgtt gtaggttcat ctatagcaag agaatcagat gatgtattct
    12541 atacttgggc aggacctgaa gtagcagttg catcaactaa ggcatatact actcaaataa
    12601 cttctttata catgatagct ttagactttg ctatcaaaaa aggaacaata actagagaat
    12661 tttatgatag tatgatttct aagatgaaag aaataccatc aaaaatacaa gaaatactag
    12721 acaatgaaga atatataaaa gaagttgcta aaacagttgt tagttctgaa catgcattct
    12781 atttaggaag aggtatagac tatagcttag caatggaagg ttcattgaaa ttaaaagaga
    12841 tttcatatat acatgcagaa gcttttgcag caggagagct aaagcatgga acaatagctt
    12901 taatagaaaa aggaactcct gtaattgcca tagctactca agaaaagtta tttgaaaaaa
    12961 tggtttctaa tatggaagaa gtaagagcaa gaggagctta tgtagtagca atagctcaaa
    13021 gccataataa ggatgttgag aaagctgctg ataaaataat ctatatacca aattcagatg
    13081 atattttatc accaatatta gcagttgtac caatgcaatt acttgcttat catgtctctg
    13141 tactaagagg atgcgatgta gataagccaa gaaatctagc taaatcagta acagttgaat
    13201 aaaaacagat taaaaattaa atagataata tttattgaaa agaactcatc ctgttatttg
    13261 tattttatag gatgagtttt ttaaataaat attaaatttg ttttgttttt gctataataa
    13321 attttataaa aagattgata ttaaatcatc tgtaaaatga taaatatata gaccatacca
    13381 ggtataggga acgataaata tagttcatat aaaatatagt gagtacttaa taaacatata
    13441 gtcaatatca gataaaaaaa ataatagctt atgagaaaaa ttaaaataca aggtattagt
    13501 aaccttgtga taagataaag tgagtagttt aaaataagaa tataaggaac tatttataaa
    13561 agaatataat gaagctaata aagtataaga aaattacttt attttagtaa aataatttca
    13621 aataaaaatt ttttaaattg ggagtataaa ttatgacaaa tgattttaaa aaagaaaaga
    13681 aagaagatta ttttgttaat cttaaggcaa tatctactga agttttacaa gaaaaagtgc
    13741 aagataacgc ttgggttata gttgatactc gcttaaatga tgcttataat ggctggaagt
    13801 tagatggagt aaaaagaggt ggacatataa aaggagcagt agatttttct gctaattggt
    13861 taagtgtata ctcagataga aaagatgaag tgctagaaca agcattgaaa actaaaagaa
    13921 ttgatttaga taaaaatata gtattatatg atgcaaatgg aaaagatgct ttggttgtag
    13981 cagattattt atctaaaaaa ggatataaat atctatataa atatgatatt aaacaatggg
    14041 cagacaacga aaatcttcca atggaaagat ataaaaatta tcaaatgata gttcctgctt
    14101 ttataataaa agatatatta gatggaaaaa ttcctgagac atttgaagat tcaaagaata
    14161 taaaaatgat tgaagcgagt tggggagaag aatcgtatac aaaagggcat atacctacaa
    14221 gtgttcatgt aaataccgat attatagaac ctcctccaac atggatgctt gataatgatg
    14281 ataacttaat taagtttgct ttagactatg gattaacaaa ggatgatact gtaatagttt
    14341 caagtagtac acctatggca tcttatagac tagctgttat attgagatat ataggagtaa
    14401 aagatgtaag agttttgaat ggtggaacaa actcatggtt atcagcagga tatgaattag
    14461 aatttactag taatccaaaa cattcatgta ctaattttgg agcagatata ccagttaact
    14521 cacaattagt tgttactacc tctgaattaa gacaaaaact aaaagaaaaa aataagttta
    14581 tactagtaga taaccgtact tgggatgaac atataggaaa agtatctgga tatacatatt
    14641 atgataaaaa agggcgtata ccaggagctt tatatggaca ttcaggtagt gatagtgttt
    14701 cactagaaga atatcgtaat attgataata caatgagaaa taaatatgag attttagaaa
    14761 tgtgggacaa ggaaaatata gatgtgaata gacagcttat atttatgtgt ggaagtggat
    14821 ggcgtgctgc tgaagtatta acatacgcaa atgtcatagg agtggaaaat acctcccttt
    14881 atagtgatgg atggatgggc tggagtcttg ataattcaaa tttaattgaa gtgggagaac
    14941 acaaataaag tttttgaaag tacgatagag ttgtttaaaa caaaggtgtt tttgatttat
    15001 tgggaagagt gtgtaaattg tgttttggga agtgtactta ggtatgttca tttggagcta
    15061 tggaaattga taaaaaagta ttatctgttt attttgctgg taaaatgtga atagattata
    15121 gattatagat tatagattat agatttaagt atagactaag taatttatat tacagaagag
    15181 aatatataag aactagtgca agatgtattt gatacatata aaaaattggc aaatccagaa
    15241 taaggataga gaaaattgag attgaagagt ttgaaagaga cttattagat gaaaaataca
    15301 aagatgtata aaaatacata ttgagaaagt atatctctct taaatttgtt aataattcta
    15361 ttatacagaa aaacatattt aagggggatt ttttactatg aaaagagtaa aattagctat
    15421 aagctttttt atattaatga ttatagggat gacaatatct tatgcagata caagtataaa
    15481 tactaaatac aatagattaa gtaaagctct tgaaatttca gaagcaaatt ttaaatttta
    15541 taatgttaaa attaattctg ttataaatca taatttatca aataaagaat ttcataatat
    15601 ttttatagaa ataattaaca atctaaagtt ggataaaaaa ggaataaatt ggaatcaaaa
    15661 agggtctggc gataaaatac aattatgtgc tactataaaa ggtagcgata aagaaatttc
    15721 tataaaagga ataaataaaa ataacaaaga aagttacata gtagttgaca tattggataa
    15781 caaagtatat aaacataaag aggttattta tagtaacgta gaaaatgtgt taaataaata
    15841 ttcatctcaa gtagatgtgt ctgcttgtat tgttggagaa tacacaaaaa ggttacaaaa
    15901 caacaaatat gatgatattt tacaaaaaat attatataat atgagtgcta agcaaataga
    15961 tgaagtgaaa gacgtaaact ttttatctat aacagcatat agtaaagtat taaatgatta
    16021 tgaattagaa tatttagggg aaaaaattaa tttaaatata ggaattaaat ataatgagga
    16081 cgacggaaga acactcatat atattgccac accaattata aaattagatt attaattatg
    16141 aggagatcaa aaaatatggc taagataata gtaaaaaaga gtaatccact taaaggtagt
    16201 gttaaaatag atggtgcaaa aaatgcagtt ttaccaataa tagcagcgac tttattagca
    16261 aacgggaaat caacattaaa tggagtacca aatttaagag atgttcatgt tatatcagat
    16321 ttattaagac atgtaggggc agaagttgaa tataaagaaa atacacttac tgttgatgca
    16381 agtaatataa aaacttgtga agctccatat gagcttgtaa gaaagatgag agcttctttt
    16441 ttggttatgg ggccattgct tgctagattt aatagtacga aaatttctat gccaggagga
    16501 tgcgctatag gaacaagacc tatagattta catttaaaag gatttaaagc cttaggtgca
    16561 aagatagaaa tggaccatgg ttttgttgaa gcagcaacag aaaaattagt aggaaataaa
    16621 ttatatttag atttcccatc tgttggagca actgaaaaca taatgatggc agcttcttta
    16681 gcagaaggga ctacaataat agagaatgca gcagaagagc cagaaatagt agatttagca
    16741 aacttcttga acgaaatggg agcagatgtt aaaggtgcag gtactaatac tataaaaata
    16801 aaaggtgtta aagagttaaa aggagcagaa cataatgtaa ttccagacag aatagaagca
    16861 gcaacttata tggttgcagc agctatgaca aaaggtgata taactgttga aaatgtttta
    16921 atggagcatt taaaaccagt tgtagctaaa ttaagagaag ctggttgtga aataacagaa
    16981 atggacaatt ctgtaagagt tgtaggtcca aaagtattaa aaccaataga tataaaaact
    17041 ttaccacatc caggatttcc tacagatgtt caagcacaat ttatggctat gcttactgta
    17101 gctaatggaa ctggtgtagt tatagaaact gtttttgaaa acagatttat gcatgttgct
    17161 gaatttaata gaatgggtgc taatataaaa atagatggaa gaagtgcggt tgttaatggt
    17221 gtagatgagc tacatggtgc agcagtaaac gctactgatt taagagcagg ggcagccctt
    17281 attttatgcg gtcttatagc agaaggagaa actcaaatag gagaaatata ccatatccaa
    17341 agaggctatg ttgatataga taagaagata acagcattag gtggtcaaat cgaaatagta
    17401 gaagattaat aattggttta tagaagaaag ttataaatta atagggttgt tcatatactt
    17461 aaatacaagg ttttaaaagc taaggaggag agtatatgaa gaacccattg gttgttttgt
    17521 taggatttgt gacttgctct gtgttagttc catcacttat aactttagtt tcttataaaa
    17581 atgtagaatt aactgaaaaa gcagagagcc cagtatctat aaacaaaact attaaaaaaa
    17641 gtgatattga agacaaaggt aataaagaag aaaaaaatac agtgaactat gaaactgtaa
    17701 ataaaaaagc accaattatc aatgtatata atcatataac agggaaaacc gaaaagatgg
    17761 acatggagaa ctatttatgt ggagtactag ctggtgagat gtcttcagag tttgatatag
    17821 aagccttaaa agctcaatct gtagcagcta gaacatatgt agtatataaa caggaacatg
    17881 gtaaatctag taaacataaa aatgcagtag tatgtactga ttataaacat tgtcaagaat
    17941 ataagagtta tgatactctt aaaaaattaa atggtgaaga atggattaag aataaatatt
    18001 ccaaaatcca agaagcagtt agaggaacta aaggtcaaat aataacttat aacgataagg
    18061 cgatacttcc tctttatttt tctacatctt caggaaaaac agaaaatagt gaagaagtat
    18121 tttctgcaaa atatccttat ttaaagtctg tggaaagtcc ttatgataag tattccccta
    18181 aatttgcgtc aacacttaaa ataagcaaca cagattttgt taaaagtctt agaagggcat
    18241 atagcactat tgtgatagat gtgaataatt taagtaaaca agtttcaata actaaaagaa
    18301 gtgatgccgg aactgtagaa aaaataaaat taggaaataa agaattgact ggaaaagaca
    18361 ttagaactgt ttttaaattg aactctgcaa actttgacat aaaatttgga gaaggataca
    18421 tagatttcgt ggtaaaagga tatggtcatg gtgttggtat gagccagtgg ggagcagaag
    18481 gaatggcaga agagggctat aaatattatg atatattatc acattactat acagatacaa
    18541 aaataaaaga tatatactaa taattgtata aaatgccaaa aacacgtcaa taatccaaat
    18601 gtagaaatat atttggaggt gcaaaatgaa gaaaaagctg ttagaaaaag atggttttta
    18661 tttatctttg tttgtatgtg tttgtttatt agcagtaggt ggagtttggt ttacaaataa
    18721 taatgtagat aaattggctt ctaataaagg aattatggaa aatgctaata aagatagtga
    18781 agaggaaata catttaattg aaaaagacaa gaaagatgct atccctactg caacggattc
    18841 aaaacaaaac ttagaaaaag ctaagtcaaa agaggaaaat aaatcgagta caacaaaatt
    18901 aaattacatt ggagataaag taataagagg atattcagaa aaagaaccta gttattctaa
    18961 aacacttgat gtttgggaaa ctcataaagg tgtagatatt agttgtacta aaggcaaaga
    19021 agtaaaatct ctattaaacg gaatagtagt agatgtattt aatgatgaag aatatgggca
    19081 atcagtcaaa ataaaaagtg acaataacat tgtagtagta tattcaaatc tagataaaaa
    19141 tgttagtgtt aagaaagaac agaaggtaac agaaggacaa tctctaggta ctgtaggtag
    19201 tacttctcaa atagaaagtg aagaaggtat acatgttcat ttagaggcgt atagtggaga
    19261 aaaatctata gacccaatga gtctaattaa gtaaaagtat aacaagttta atcatatttg
    19321 gttaagcttg ttatattttt tggtgttttt acatatatat cattaaagct attatgtgag
    19381 gggggtaatc tttgagatct catatagagg aaagggccat agtggtagca aaatatatac
    19441 ttgaaaaaaa cactacagta agacaaaccg ccaagacatt tggagtaagt aaaagtacaa
    19501 ttcataagga tgttacagaa agattgaagg aaataaatcc atcccttgcc aaagaagtta
    19561 aaaatgtgct ggacaaaaat aaatcagaga gacatattag agggggatta gcaacgaaat
    19621 taaaatatga gaaggaacat aaaaaaatgt aggttaaaaa cctacttttt ttatttaatt
    19681 aaattataaa aagaaatata ttaagtaatt ataaatagca aaataataat actctcaaaa
    19741 gatttatttt ctcttgatta aagcgagatt tttgtatatg ctttattata ggagtattaa
    19801 aataggctta taatataatc taatattata aataaaggag ttgaaaaaat ggctggagct
    19861 gatataggca tagatttagg tactgcaaat gtgcttgttt atgttagtgg taagggaatc
    19921 gttttagaag aaccgtctgt tgtggcgata gacaacagaa cagattctgt tttagctgtg
    19981 ggagaagaag ctaaaaagat gatagggaga acaccaggta atatagtcgc tataagacct
    20041 ttaagagatg gtgtaatttc agattatgat gtaactgaga agatgttaaa atcttttata
    20101 gataagatag ttgataaaaa gggatttgga agatttttta tgccaagaat tatggtatgc
    20161 gtaccaacag gtgtcacaga agtggaaaag agagcagtag aagatgctac gagacaagct
    20221 ggagctagag aagtatatat aatagaagaa ccaatagcag cagccatagg agctggtgta
    20281 gacatatcac agcctaatgg taatatgatt atagatattg gtggaggtac tgtagatata
    20341 gctgtaatat ctctaggagg agctgttgta agtgaatcta taaaggttgg tggagataga
    20401 tttgatgatg ctatagttaa atatatgaaa aaacaacaca atcttcttat aggagaaaga
    20461 acagcagaga aaatcaagtt tgaaataggt tctgcattta agagagaaga agaaaaatat
    20521 atgaagataa caggaagaaa tttaattaca ggacttccta gttctataac tataaattca
    20581 acagaaatgt tggaagcact tagagaatgt gttgagcaaa tagtagttgc tactcatgct
    20641 gttttggaaa aaacgcctcc tgagttagca gcagatatag gagatgttgg tataataatg
    20701 actggaggag gttcattatt atatggatta gataaaataa tagaacagag aacaggaata
    20761 actgttacaa tagcagatga accattatct tgtgttgcaa aaggtacagg aatagcttta
    20821 ggttcaatag acctacttga aactggcggg tctttcaaaa gaaaataatt aggagagttt
    20881 agaatgttaa atatagatga aataaaaaaa ttaataccac atagatatcc ttttttatta
    20941 gtggacaaga ttacagagct agaagttggg aaaagagctg ttggtataaa gaatgtaaca
    21001 gttaatgaac catttttcca aggtcacttt ccagaatatc ctttaatgcc aggagtatta
    21061 atagtagaag ctttagcaca agtgtgtgga gtggcaatga tgtctgtaga ggaaaacaaa
    21121 ggaaaactag gtgtatttgc aggtatagat aaagttagaa tcaagagaga agtaagacca
    21181 ggagatacat taactatgga agttgaaatg acaactctta gaagaaatat agccaaggca
    21241 gatgctaagg catatgttgg tgaagaactt gtctgtaaag gggaattaat gtttgcctta
    21301 gtagaaaaat aaaaacttga aatataaaag taaaaaggta gataatttgg agtatttaat
    21361 cagatactct aaaatatcta cctttttata taaagaattt tatcatttag tttatattta
    21421 gattaggtgg atactaataa agccaaggct attgtttcag ctaagtctaa tataaagcta
    21481 agacgtatat tattaaatga aagtttatta ttttcatcaa ttttatctac aattcctact
    21541 atagaataat ttcctatttg aggtagtttt ttgccaacac cttttccggg caggataggt
    21601 ccttctctaa tctgtatatt acctatgtta ctctgcgacc ccaagcaagc atctatagcc
    21661 aagaagttac cttgtatatg tgtatttttt atagtatcta aagactcata tatatttaag
    21721 gcgtgaatag ggttatctag tgtaccgtaa acggggtatt taaagttact gttcttaagt
    21781 attgttccaa ctaaagggcc taaagtatca cctatagccc tgtctgttcc tatgcaaaca
    21841 acaatcgtat tttcattaat tatagttttt agaactgaac ttagcatctg tataacatca
    21901 tcatcgctgt aatttacttt agctaaatac aataggaaca cccccttcaa aaaaatatat
    21961 gaataaaaag cattaaaaag acctaacttt aaatttcaga tagatagaaa attttatttg
    22021 tcttacaaaa aaaggaatag aaaaaatatg actaaaaata ttgacaaaat tttaaaaaaa
    22081 tagtaagcta gtaaagtaaa acaaaactta aatcttgatt accttatcaa gagaggctga
    22141 gggataggcc ctgtgaagcc cagcaaccac ccaaatggga aaaggtgcta attcctacaa
    22201 gacaaaagtc ttgaaagatg aggtcagtta attagtatgt aattacgatt attaacctct
    22261 tcttataaag gagaggtttt ttattttgag taaatttcta aaattaaaaa taaggtaaaa
    22321 cctataataa aaactaagaa taaataaggg ggaataaaaa tggcaagaca tttatttacg
    22381 tcagaatcag ttacagaagg acatcctgat aagatatgtg accaaatatc agattcaata
    22441 ttagatgcat tattagaaaa agacccacaa tctagagtag cttgtgaaac tacagttact
    22501 actggtttag ttttagtagc aggagaaata agtacatctg cttatgtaga cattcctaaa
    22561 ttagtaagag aaacagtaag agaaattgga tatacaagag caaaatatgg atttgattgt
    22621 gatacttgcg cagttataac ttctattgat gaacaatctg gagatatagc tatgggagtt
    22681 gatgaaggcc tagaaagtaa gactggagaa gagatagaag aagaaataga aaaagttgga
    22741 gctggagacc aaggtataat gtttggattt gcatgtaatg agactccaga attaatgcct
    22801 cttccaatat ctttggcaca caaattatca agaagactta cagaggttag aaagactggt
    22861 ctagttgatt atttaagacc agatggaaaa actcaagtta ctgttgaata tgaaggaagt
    22921 aaagctgtaa gagtacatac agttcttata tcagctcaac attgtgaaac agtatcaaat
    22981 gataaaataa gagaagattt aattaatcat gttattaaag aagttatacc agcagaatta
    23041 cttgatgaag atactaaaat ttatataaat ccaacaggaa gattcgttat aggaggacca
    23101 caaggagata caggtcttac aggaagaaaa ataataatag atacttatgg tggatattct
    23161 agacatggtg gaggagcttt ctcaggaaaa gaccctacaa aggttgatag atctgctgct
    23221 tatgcagcta gatatgttgc aaaaaatatt gttgcggctg gtcttgcaga caaatgtgag
    23281 atagaacttg catatgctat aggtatagct agaccattat ctatatttat agatactttt
    23341 gggacaggca aagtttcaga agaaaaactt gttgaattag taaataaaca ctttgattta
    23401 agaccaggag ccataataag agacttagat ttaagaaagc ctctttataa aaaggttgct
    23461 gcttatggtc actttggtag aactgatata gatttacctt gggaaagaac tgacaaggtt
    23521 gagcagttaa gaaaagatgc tttaggtgaa taatttttaa ctaaaaagct gttagaatgt
    23581 aattttacga ttactttcta acagcttttt tattatgagt taaatatttt taataaatgt
    23641 taatttatca tcatataatg aataaacagt atttaatctg gtcaatacat tttatataat
    23701 actatactaa atttatgcat attatgggat atatgttaaa ggatggttaa gaatgtgtta
    23761 actaatgtta aataattaaa aatatattga aatatcataa taatgagtct aatataatct
    23821 taataaactt acactttaaa aataatttta attgggggga gtagtgtatg tcaggcaaag
    23881 taaatattaa aacggttata agttttgctg gagcttatgt tgctactgta ataggttctg
    23941 gatttgcaac tggtcaagaa atactgcaat tttttacatt ttatggatat gctggtatta
    24001 taggtggaat aatctctatg gtactatttt catggtttgg agcagaggtt atagataagg
    24061 gaagagaatt gaaacttaag gagccaataa aaatttatca agtatattgt ggaaaatatc
    24121 ttggaacatt ttttgaatgg tttggtccat tgtttttatt tggaacatta gtaataatga
    24181 ttgcaggagc aggagctaca ttatcagagt actatggatt aaatccatat gtaggtagga
    24241 ttggtatgac aatcgtttcc ctaataactg tttcattagg tttgacaagg ctttctaaaa
    24301 tactaggtaa tataggacct ataataataa tatttacact tttagtagga gcaattagtt
    24361 tatttagcaa tattgatgct ttatcaaatg ctggaaatat ggttgatagt ttaaatatta
    24421 aaactgctac atcaaatgga tatttctcag gagtacttta tacttgttat aatgtaataa
    24481 tagtaataac attcttgaca ggaatgggag cttctgcagt aaataagaaa gatgctgtgt
    24541 ggggtggtat agtaggtgga gtagcactta tggcagcagc tataatgatg aatttggcat
    24601 tactttcaga cataggaaat atatacacaa aagagatacc agcactttac ttagctgata
    24661 agatatctcc aataataggt attttattct cagtagtact tttactagga atatacacta
    24721 cagctgtacc attgttatgg tcagtaacta accgatttgt agaagatgac caccctaaat
    24781 ttaaaattat aacaatagta gtaagtatat tagcttgtat aggaggtcta ctgccatttg
    24841 ataagttagt aggtacacta tatccatata caggttacat gggaatttta attttactat
    24901 gtatgcttta tagaagaatt actaaaactg aggggtacaa ggagaataaa tctgaaataa
    24961 gctagtactg taatatactt atgaaattta atttatactc taaatatgga gtaattaaaa
    25021 taaaatagct atttaaatat ggtacctgta tttcatggta ccatatttta tggatgcaga
    25081 ttttttttgt ttctgataaa atgtataata gttggaaatt acgttaagta taaaaatata
    25141 aaacttttct acgattatca tagttttaat atataaaatt atactaacat ttaaatggag
    25201 cgattattag tatttttagg agataaaaat ggaaaaatta gaaggaatga taagtgaaat
    25261 agtctttaaa aacgaagata atggttatac aatagctcat ctggtaaatg aaaatgatga
    25321 aattgtcgtt gtaggatgta tgcctacttt ggcaattgga gaaagcatag aagttgaagg
    25381 aaagtgggta aaccataaga tttatgggac tcaatttgaa gtgaatagtt ttatgccagt
    25441 aacaccatca tccttagaag gaatttatgt ttacttatct tctggaatga tacatggtat
    25501 tggtgaaaaa atggctaaaa gaataattga taagtttggt gtagatacgt tagaggtgat
    25561 acaaaattca ccagaaaaac tacaagaagt agaaggtata gggagtaaaa aagtaaaaca
    25621 aatagtaaaa agttatgaag aagacagaga attgagaaat ataataatac agctatctcc
    25681 ttttggaata acaccaaatt actgcctaaa aatatataaa aaatataaaa gcagtgcaat
    25741 agaagttata aataaaaacc cataccaact tgctgaagat ataagaggaa ttggatttaa
    25801 agtagctgat agtatagcaa ataaaattgg tatagataaa aattctaagg atagaatttg
    25861 tcagggaatc ttatacactt taaataaatc tttaagtaat gggcatacat atttaccaga
    25921 gcatgttcta atacaagatt cggaaaaatt attggagcta aatggagaaa taataaaaga
    25981 gtgtgtaatg atgctagttt ataatcaaaa aatacatgta gagaaagtaa acaatgaaaa
    26041 tcttatatac cttatgccat actatttagc tgaaaatgga gtatgtagtc aaatagtaaa
    26101 attatcacag tatgaatttg aagacttaaa aatagatata gataacgaga ttaatgtttt
    26161 agaagaagat aagaaaatta agttagcaga aaaacaaatt ttagcagtca aagaatctgt
    26221 taatagtggc gtattaatta taaccggtgg tccaggaact ggtaaaacaa ctaccattaa
    26281 tgctatcata gatatatttg aaaataatgg taaaagcgtg actttggcag ctccaacggg
    26341 aagagcagca aagagaatga gtgaaacttc taataaagaa gcaaaaacta tacataggtt
    26401 gctagaaatg ggattttcaa cagatgatga tttaactttt tttaaggatg aagaagaccc
    26461 aataaattca gatgtaataa ttgttgatga ggtatcaatg gtagacatta tcttaatgta
    26521 caatttactt agagctataa agttaggaac aagagtaatt ttagttggag atagtgacca
    26581 attaccttct gtaggtgctg gaaatgtact taaggatatg attaattcta atattataaa
    26641 tgtagttaaa ttaaatgaaa tatttagaca agcgcaagaa agtatgataa ttgtaaatgc
    26701 ccataagata aacaatggag aaccacttta tctaaataca aaaggtaaag atttcttttt
    26761 tataagaaaa tcaactaatg aagaaatttt aaatgaaata ataggattgg ttaatgaaag
    26821 attacctaaa ttttataaag ttgataaatt aaaagatata caagttttat cctcaatgag
    26881 aaaaggtgaa ttgggagtta ctaatctcaa tatagagtta cagaagtatc ttaacaaaaa
    26941 agaaaaattt aaagttgaag aaagtttttc taaaagatta tttagagttg gcgataaggt
    27001 aatgcaagta aaaaataatt atacaaaaaa atgggaaaca gaagaccaaa aagaaagtgg
    27061 agaaggtatc tataatggag atataggata tgtttatcac atagataaag ataaaaaaac
    27121 tatttatata ttatttgacc agactaaaat tgtatcatac ctatatgatg aattagatga
    27181 aattgaccat agtttttgta caacaataca taaaagccaa ggaagtgaat ttccagttgt
    27241 agtattgcca atagcttggg ctcctcctat gttacttagc cgtaatttat tatatacagc
    27301 agtaacaaga gcaaaaaaat tagttgtatt agttggagat gtgaaatatc ttgaatatat
    27361 gataaaaaat aatagagtta atcaaaggta ttcaaattta ggatataagc taaataaatt
    27421 taagcaagaa gggctgttga tagaataaat atgaaaatat gcaatgataa ttttattaag
    27481 aattttaaaa atatgattaa taaatgtttg gattttatat atcctgagaa tataagttgc
    27541 ataatttgtg ataaatccat aaagaaaaca aatacttatt caatatgtaa aagttgtttt
    27601 aaagaaatga attttataca agatggttgc atgaagtgtg gaaaaccaat aatacgtcat
    27661 tcaattgaaa aagaatttat tgaagaatgt agttattgtt ttaataaaga cttttatttt
    27721 gataaatcaa tatcttgtat tgaatacaat gatgtgacaa aaaagatagt gctagggctg
    27781 aagtataacc aaaaaacctt tatggctaag tatatagctc aaataatgaa agaaaaatta
    27841 tatttagaaa acataaaatt tgattatata ttatttgttc cacttcataa aaaaaggcta
    27901 aataaaagag gttttaatca ggcacaaaaa atagcattta atttaagtaa aatagtaaat
    27961 attccattat tggattgtat aagtagaaaa aaatatacaa gaatgctata taaattgaat
    28021 aagaaagaac gaaaagaaga attaaaaaat gtatttgttg ttaaagacaa tgttaaatta
    28081 ataaataata aaaatatact tttaatagat gatatattta ctactggtct aacaacaaac
    28141 gaaatttcta aacttttaaa gttatcgggt gctaataagg tttttgtatt gactctactt
    28201 acaaaagcaa atgataatta tattatggaa taatcttata tattgacaat tttaatttaa
    28261 aatattaaaa tgaataagtg atatttaggt ctgtggttga aagtccaagc cagtcgcagg
    28321 caaagggatc cacgtaagtt aacactaaaa attgggttaa tgatcatggt gcggcttaga
    28381 agtaagacct accgataata aaatcgagag agagtagtag tgcggcgaac ccaagcgaac
    28441 cctccagtag gcgagtgtgg ggtcaaaaac caggtcagct aaatatcgta ttaaaaggga
    28501 gctagcatac ctttatgtat agctcttttt tatatataat aaatttaggt tgagtcataa
    28561 agaggtgata tgtttgaata aaagacagga aaacttatta acaacgttgc tattaaaaga
    28621 agagtttata aatgttaata agattgccaa tgattttgag tgttcagaaa ggacaataag
    28681 aaatgattgc aaatatattg atgaatggtt atctactttt tgtaatgctt atatcaaaag
    28741 aaaacctaat attggaatta aatttagtgg agatggaaca gacaaaatac tggtaaatca
    28801 gaagttgcga ggtgaagtta aaagagtaaa tagtgagctt cataagcaag ttgagatatt
    28861 aaatttacta ctttgtaata ataaaaaagt tactttaaat gatttatcta ataagttgta
    28921 cataaataaa aatattgtca gagaagaaat aagtaaatta tcagtggcat taaataatta
    28981 taatttaaat gtctcgacta aaaaaggcag tggtatatat attggagggg atgagaaaga
    29041 cataagaata atgttagtat cattcttatt taaatacata gataaataca gattaaacat
    29101 agatgaaata gagtacttcc atatagtaga tataataatt gtaaaaaaca taatgtttac
    29161 tatagaggag gatatggata taagacttac agatatatca ttcaagcaat tgatgctatt
    29221 tttacttata aatataatac gaataaggct aggaaattct ttaaaagtat caaatactaa
    29281 gaaagagctt aagctaaaaa tgctgatatt agatatagaa gatgaattga aatctaattt
    29341 atccatagta ttaaattatg aagaaaaatt gtttatagag tctttaatat tgggtatgaa
    29401 caaacaaata aatagtagta atattaagaa atctttatat ctaaatgaag aattagcaag
    29461 ttatacaaaa aaaattataa atttagtatc agaagaatcc ggaataaatt ttaataatga
    29521 tatattactt tatgaacaat taatatgtca tttgaatgtt acaatgcacc aaatgaaaag
    29581 caatgtttac ttagaaaatc cacttttaga taacataaaa actaaatttt gttttttatt
    29641 tacagtaata tcaagttcaa tagaaattga attaaaagga aaacaaataa cagaagatga
    29701 aattggttat cttacacttc actttcaaac ctctctagaa agaaaataca gcaaaaggga
    29761 atcaaataaa aaggcaagta ttgtatgtcc atttagtttt ggagtaagca tgctattaaa
    29821 agtaaagata gaaaagagat ttgataacat aaaaattatt gaaacattaa gagaggaaga
    29881 tttaaaaaga gataacttca ataagactat agattttata attgcatttc aagagtatga
    29941 aaatatccaa aaaccaattt ttataacaac accattattt actgatgagg atgagaataa
    30001 acttaaagag ttcacaaaca aaataaaaga aaaagacact tcatataaac ttatgaacag
    30061 attaatgatg tctgattatt taataagaga gttagaacat gatgatattt atgaagctat
    30121 aacatattta actaatttat taattgaaaa acattatgca gaaaaagagt atttacaatc
    30181 tattataaca agagaaaagt catatccaac caatgttgga aaaggaatat tattccctca
    30241 tggagatatg aaatatataa aacaatctgt tttatgtttt gcaaggttaa aaacaccaat
    30301 aagaattaga aatggaaagg atataaagat tatacttttg ttagcatata aaaaagatga
    30361 tgatagaaaa gtatttagag aattatttaa agaaatatct aatttaacag aggatgaaaa
    30421 tatgctagat attctaagta aatgtgacat tgaaaaagta aaagatattc ttatataaaa
    30481 ttcatagtga actatggata agcacaaaat tttccatgtg atggaaaaag ttgtgcatgt
    30541 ttttttgttt ttataagata ttatatgttt gtaaggtata aataatctaa aagtagagaa
    30601 gcaacttagg aggaaaaaat ggaagaaata gttttaaaga ttataattca tgctggaaat
    30661 gcaaaatcta tgctttatga agcacttgac tatgcaaagg aaaacgattt taaaaaagca
    30721 gatgaattaa ttgaaaatgc aaatgaagaa attttaaaag cacataaagt acaaactgaa
    30781 cttatacaaa aggaagctgg gggagataaa tcagatatat ctattttatt gattcactct
    30841 caagaccatt tgatgacttg tatgagtgag agaaacctta taaaagaaat gattatgtta
    30901 agaaaagaaa ttcaaaaatt aaaataaaaa aattaagggg gatttaaaaa atgaaaagaa
    30961 aagtatattt attttgtagc tttggtatgt caacaagttt acttgcagat aaaatgcaga
    31021 aggtagcaga tgaacacaat ttaccaatag aagtagaggc atttccgata gcagaaattg
    31081 acaaaatagt tgaagaaaaa aatccagatt gtatattact aggaccacaa gttaaataca
    31141 tgctaaagga attaaaacca aagtttgaag ctcaaggaaa acttatagat ataatcaatg
    31201 aagttgatta tggaactatg aatggggaaa aagtattaaa actcgctata aagctaataa
    31261 aatccaaaca agcgtagaat tatagtaaaa aataatagga aatgagggta gatagatgaa
    31321 tagattggaa agaatactta tgccattggc tgaaaaaata ggtaaaaata aattattaat
    31381 tgcaattaga gatggttttt taatatcatc accattatta ataataggtt cattattttt
    31441 attagtagca aattttccta taaaaggatg gaatgaattt gtaggtcaaa tttttggttc
    31501 agattgggct gtaaaattgc aacaaccaac tgtagcaaca tttgaagtaa tgactctact
    31561 tgcagtttta ggaattggat attcttatgc aaagcaaata gatgtagacc caatagcatc
    31621 agcagcagtt gcaatagttg ccttttttat aataacacca tttgtaatac catatacacc
    31681 agaaggaaca gaatcagtct acatggtaac aggtatacca ctaggttgga tgggctctaa
    31741 gggaatgttt gtaggtatga taacagcaat aacctctgta aaattatttg ctgcagttgt
    31801 aaaaaaagga tggacattga aaatgccaga tggagttcct ccaaccgtat caaaatcatt
    31861 tgcagcatta ataccaagtg caatagttat ggtagttttc tttttagtta agatagcttt
    31921 tgaagctaca ccatatggaa gtatacatga atttatattt aagtttttac aaatgccatt
    31981 attaaaatta ggaaatagtt taggtgctat ggcaatcgct tatatattct tacatttatt
    32041 ctggttcttt ggaataaatg gaagtagtgt tgttggggct gtgtataatc caatattaaa
    32101 aattctatca gctgaaaatt tagctgcttt tcaagctggt tcaaaaatac caaacataat
    32161 aacaggtcaa ttccaagata tgtttgcgac atttggtgga gcaggtagta cactatcatt
    32221 agtcatagct atgttattaa tatgtaaaag taaacgtata aagtcaatgg gaaaactatc
    32281 aattttacca ggaatatttg gtataaatga gccattgata tttggtctac caataatgct
    32341 taacccaata ctattgatac catttgcaat agttccaaca ataaatataa taatagctta
    32401 tttctgtatg tcagcaggat tagtgcctct tacaaatgga gttcagttac cgtggacaac
    32461 tccaattata ttctctggtt ttttaacaac aggatggcaa ggtgcagtct tacaagtagt
    32521 gcttcttata ctaggaatat ttatgtatat accatttata aaaatgctag ataagcaata
    32581 cttaagagaa gaaaatgcaa ctgttgaaga ggaagatgat gatatatctt ttgatgattt
    32641 agtattatag gaggaagatg atgaagaaaa tagttatgat attggatcaa atacaagctg
    32701 gagctggagg aaaagagaaa agtaatatac ctcctgctgg aaaatcaagc ccattaggac
    32761 caggtataat gatggagcaa tttttaaatg aatctaaggt aatagctacc ttattttgtg
    32821 gggatgaatt ttttgtaaat aatgaagaag aagtaactag taagatgata gctatggtaa
    32881 aaaaattaaa ccctgatgta gtaatctgtg gaccaagctt taattacgaa aacttttcta
    32941 agatgtcagc aatactgtct aaaaatataa atgataaaac agatatacca gcttttgcag
    33001 ctatgtcaga agaaaatata gatgtgataa acgaatataa aaatgacata tgtatagtta
    33061 agactccaaa aaaaggtgga attggtctta atgattcatt aaataacata tgtaaattgg
    33121 caaaagcaat agcaaataaa gaagatataa ctttgatgaa agaagaattt tgttattaat
    33181 aagagtatta attattagta agtttaaata ttatactatt tataataagc atacattagt
    33241 aaaatctata taatttaagg aggggaattt taacatgtgg ggaatgatag cgacatggag
    33301 aatggcccta gaaggaataa ctgaggcatc agaacattta gaaaaaggca tggaagcagg
    33361 tgatgctgta gagcttgctg taagaagagt tgaagactat ccattctata aatcggttgg
    33421 atatggtgga cttccaaatg aaaattgtga ggtagaactt gacgctgcat ttatggatgg
    33481 taacacatta gatattggag cagttgctgg tataagagat tattctaatc ctgtcagcat
    33541 agctaggaag ttaagttatg agaaggtaaa taatttttta gttggaatag gtgctgaaga
    33601 ttatgctcat aaaaatggat ttgaaagaaa aaatatgttg actgatagag cgaagttaca
    33661 ttataaaaag agaaaaaaag agactttaga caaaggatta agtccatatg ctggtcatga
    33721 tactgttggt atgatatctt tagacaaaac tggaaaaatg tgtgcagcta catcaacaag
    33781 tggattgttt atgaagaaaa gaggtagaat aggagattct gcactatctg gttcaggatt
    33841 ctatgttgat agtagtatag gaggagctac agctactggt ttgggagaag atttaatgaa
    33901 gggatgtata tcttatgaga tagttagact tatgaaagaa gggaagcatc cacaagaagc
    33961 atgtgatatt gcagtaaatg aacttgataa acagcttatc aaaagaaggg gaaaagctgg
    34021 tgatttatca ttagttgcca tgaataataa aggtgaatgg ggagtagcta caaatatagc
    34081 tgattttact ttttcagtag taactaaaga agaatctcct actgtatata tttcaaatag
    34141 agtagatgga aagactatac ataaggttat atcaaatgag tggatggaag catatgaggc
    34201 tagaataaaa gcaccaatag aagtaagtga gtaaagtagt ggttacataa ttaaaataga
    34261 gttttataat aaggagattt ttatgaatat cataagtaat aaaatagatg agttaaaaga
    34321 agatttatta agtgatatta tagatattgt aaaaatacct agtgtcaaag gggagtcaga
    34381 aaatggcttc ccttttggcg aaaaagtagg ggaggcactt aataaagcat tagaaatatc
    34441 agaaaaattg ggatttaagg ttagaaactt ggataattat attggttatg cagagcatgg
    34501 agatagtgat gattatgttt gtgtaattgg acatgtagat gttgtacatg aaggagatgg
    34561 ttggaagcat cagccttata agggagaaga aactaatgga agaatttatg gacgaggagt
    34621 actagacaat aaaggaccta taatgtctgc cttatatgga ttatatgcta ttaaagaatt
    34681 aaatttaaaa ttagataaga gagttagaat aatatttggt acaaatgaag aaagtggatt
    34741 tgaagatata ccatattatt tagaaaaaga aaaagcacct attatgggat ttacaccaga
    34801 ttgtaaatat cctgttgtat atggtgaaaa aggcatggca aagattagaa taaaaagtaa
    34861 aatcaattat gaagaagatg tatacttagg ttttatagaa aatatgtctg aaaatgtgct
    34921 tgttacatat aaagaactga atatagaaaa tagtgatact atattagata taaaggtaaa
    34981 atacgatttt agttataaat tgaaagatgt attggatgaa ataaaagcta gttttccaaa
    35041 tagtatcgat atagaggtaa tttctaattt taatcctgta tattttgata aagagagtaa
    35101 tcttgttaaa aagcttcagc tagcttatga aagggttaca tcattagatg gaactccagt
    35161 aacaactaat ggaggtacgt atgcaaaagt aatgccaaat atagttccgt ttggaccttc
    35221 atttccagga caaaaaggaa tagctcataa tcctgatgaa tacatggata tagaagatat
    35281 aatattaaat gcaaagatat ttgcaaatgc aatatatgaa ttagcaaagg agtagttatg
    35341 ttagaaataa taggaatgtc agtagaagac gctaaaatta tagaagattg tggtgcagat
    35401 agaatagagt tagttagtgc tttaactgaa ggtggattga caccaagttt tggtcttata
    35461 gaaagtgttg ttaacagtgt aaagatacct gtgaacgtaa tgataagaca tcatgcgaaa
    35521 agttttgtat acagtaaaga agatataagt ataatgcaaa aagatataag tgtggttaaa
    35581 gagattggtg ctaatggagt tgtatttggt gtattggata aaaacaatag tatagatgag
    35641 aaaaatctaa atgttttatt gaaatgttgt gataatcttg atgttacatt tcatagagca
    35701 atagatgaat caaatacaat agatagtgtt aaaattttga aggattatga taaaattacg
    35761 aatatattaa catcaggagg gaaaggaagt atagttgata acatacaaat gataaagaat
    35821 atgatgctta gttctaatca cataaaaatt cttttaggtg gaggtcttaa ttttaataat
    35881 atagaaaaga taaaggaatt aactaaggca agtaattttc attttggtac agcgattaga
    35941 ataaataaca gtccttttga agatatagat agacaaaagc ttaaacaact cgtaaatata
    36001 atttcaagat agtatatgaa tataaaatac ttagtttaaa taaagagagt aagttaatta
    36061 taatttgggt atatgaatgt taataggtat taaatatgaa attattgttg agtatgcttt
    36121 ataactttta agtagtttta aatataaata atgaaagaaa agatagatta tggaggtttt
    36181 agagggttta tatttattaa aaaattataa aggtatattg gcatataata cacataattt
    36241 cctaaaatta ccatcttatg acatgaattc acatattaat aaacaattaa tggagatttt
    36301 tgtggataga aataggagta aaaatagctt taaataaaca aaaacaagag ttattaacag
    36361 ttttatccac attatccaca ggtgtttgta ataatattaa tccacataag tatcaatgta
    36421 actaaaaatg ataaataatt ctatcaattg aatagaaaaa atcataaaag taaataataa
    36481 aaagaggatt atgagagttc gtgtagaata tattattaaa gtataaattt tcttatactg
    36541 gcataattga agggagatgt atttatgaac ataattatat ctggaaaaca aatcgaatta
    36601 actgatgcaa taaaaaataa aatcgaaact aaactaagta agttagataa ttacattcat
    36661 ccagacactg atgtaaaagt tacagtaagt gcgagaaaag caagacaaaa aatagaagta
    36721 acaatagcta ctgtaaatgg tcctataatt agagcagaag atatgcagga aaatttatac
    36781 acagctatag atgttgtata tgataaatta agtaaacaat taaaaaaata taaaaagaga
    36841 ttacaagata aacataaaga caacaagagt ataagattcc aagatgcaga gataaatcta
    36901 gctgatgaac aagaggatta tggggatgaa aatcaagaat tagtaattca aagacgtaag
    36961 aaatttagtg ttaagccaat gagtgaagaa gaagctgtat tacagatgga attaatagga
    37021 catgattttt atatgttcaa gaacatagat tcagatgaaa tagcagtagt ttataagcgt
    37081 aataatggtg gatatggtat aatagaacat gaataaagca atttagattt aatcatatta
    37141 gctatcgata aataaattat cgatagcttt ttaaattaat attaaaagta tagtttttat
    37201 ggtaaaatga ataatagtac aataaagaac gaggagagat gtaaaatatg agctttatgg
    37261 ataatttatt caacatggca gataaaaaag aattaaaaaa gttcaataaa actgttgata
    37321 taatagattc attagaacct aaatttgaat ctatggcaga ctcggaactt aaaaatatga
    37381 caaatatatt taaagaaaga ttagcaaatg gtgaaagtat agatgatata cttccagaag
    37441 cttttgcagt agttagagaa gtttctaaga gagtgttagg acttagacac tacagagttc
    37501 aaatgattgg aggtatagta ctacatcaag gaagaatagc agaaatgaaa acaggtgaag
    37561 gtaaaacttt ggttgcaact gctcctgttt atttgaatgc gcttactgga aaaggtgtac
    37621 atgttgtaac agtaaatgat taccttgcaa aacgtgatag agaccaaatg gcaaaaatct
    37681 atgagttttt aggaatgagt gtaggcgtta ttatacacgg tcaaaatcca aaagttagaa
    37741 aagaacaata tgattgtgat ataacttatg gtacaaataa tgaatatgga tttgactatc
    37801 taaaggacaa tatggtaata cacaaagagc aaagagttca aagaggatta aattatgcta
    37861 tagtggatga ggttgactct atactaatag atgaggctag aacaccactt atcatatctg
    37921 gacctggaga caaatcaact cacttatatt cagatgcaaa cacatttgtt ttaaccttaa
    37981 aaccagatga ttatgaatta gaagaaaaag ataaggctgt atctctaaca gcatcgggta
    38041 tacaaaaagc agaagtgtat tttaatgttg ataatataac agatatatct catacagaat
    38101 tataccatca tataaatcaa gctttaagag cacatgtaat aatgaagaaa gatgttgatt
    38161 atgttgctaa agatggagaa atagtaatag tagatgagtt cacaggtaga cttatgtttg
    38221 gtagaagata ttcagaaggt ttacatcaag caatagaggc taaagaaggt cttaaaatac
    38281 aaagagaatc taaaacacta gctactgtaa cattccaaaa ttattttaga atgtacaaaa
    38341 aactttctgg tatgactggt acagcaaaaa cagaagaaga agagtttaaa gcaatctaca
    38401 aaatggatgt tttccaagta cctactaata agcttatgat aagggaagac ttaccagatt
    38461 gtgtatacaa aagtgaaata ggtaaattta atgctgtagc acaagaaata attgaaaggc
    38521 acaaagtcaa tcaaccaata cttgtaggta cagtatcaat agaaaaatca gaattattat
    38581 cacaaatact aaagaaaaag ggcataaagc acgaggtatt aaatgctaag catcatgata
    38641 aagaggcaga aataatagca caagctggta gacttggagc tgttacaata gctactaata
    38701 tggctggtcg tggtacagat atagttctag gtggaaatcc agacttttta actaagagag
    38761 aaatgagaag aaatggattt aaagaagaaa tagtaaatag agtagatact ccaatagaag
    38821 gtatacctgt aaaaggtaat gaaattctat ttgaagctag agaagagtat gaaaaactat
    38881 ttgaaaagtt taagcaacaa acacaagaag aacaaaaaca agttgtagaa gcaggtggac
    38941 ttgctataat aggtacagaa agacacgaat ctcgaagaat agataaccag ttgagaggtc
    39001 gtgctggacg tcaaggtgac ccagggagtt caagattcta tataggtctt gatgatgacc
    39061 ttatgagatt gtttggtagt gatagaatat ctggtattgt agataaaata ggtcttgaag
    39121 aagatatgcc gatagaacat agaatattat ctaagtctat tgaaggagca caaaagaaag
    39181 tcgaaggtaa aaactttgga ataagaaagc atgtacttca atatgatgat gttatgaata
    39241 aacaaagaga aataatatat gcagagagaa aacgtgtatt agaaggtgaa gatttacaag
    39301 aacaaataca atcaatgact cattctataa tagaagaagc agttactctt tatacacaag
    39361 ataaaggatt tgatgaagaa ggatttaaag aacatatgta taatctgttc ttgccaaaag
    39421 gtagcataga aataccagaa atagaaaaac ttaatccagt tgaaataaca gaaaaagttt
    39481 atgaaatagc tatgaagata tatacttcaa aggaagaaca agttggctat gagagaatga
    39541 gagaagttga gagagtaatc ttacttcaag ctgttgataa ccattggata gaccatatag
    39601 atgctatgga ccaattacgc caaggtattg gtcttcgtgc tgtaggtcag caagacccag
    39661 ttattgctta taaaatggaa ggttttgata tgtttgatga aatgaataaa catatcaaag
    39721 aagatactgt aagatattta tttaatataa caatagaaac tccagtggaa agaaaagcag
    39781 ttgtagatgt tgaaaatcta tcttctccat cagatggaac acttccaaca agtaaaactg
    39841 ttaaaaaaga tgaaaaggtt ggaagaaatg acttatgccc ttgtggaagt ggtaaaaaat
    39901 ataaaaactg ttgtggaaga taatctcgga ggattaaatg ttaaagttac aagagtatag
    39961 acatagtatg gagcagatgg cttcaaattt agaagaattg agggtttctc tttgacatag
    40021 cttcattgat gaaaaatata caaattaatg aagaaaagat gaataaacaa gacttttgga
    40081 atgataatga agtcgctcaa agagtacttc aagaaaataa gtctttaaaa gagacattag
    40141 aagaatatga aagcttaaaa agcttactag aagatataga agtattaatt gaaataggtt
    40201 tagaagaaga tgatgattct gtagaaagag atatagaaaa gtccatagaa tcaatggaag
    40261 aaaaattatc tgaaatgaaa ataaagactc ttctaaatgg agagtatgat aaaaataatg
    40321 ctatattatc tataaatgcg ggtactggtg gcttagatgc tcaagattgg gctcaaatgc
    40381 ttttgagaat gtatataaga tggtcagaaa gtaagggtta taaagttaag ctcttagata
    40441 taatatcaga ccctgaagcg gggataaaaa cagctacaat tcttgtagaa ggaacaaatg
    40501 cttatgggta tttaaagagt gaaaaaggag ttcatagatt agtgagaata tctccatttg
    40561 acccatcagg aaaaagacat acttcatttg catctataga tgtaacacca gagttagatg
    40621 aaaatataga agtagaaatt aatccatcag atttaaagat agacacatat agagcttctg
    40681 gagctggagg gcaacatgta aatacaactg attcagcagt tagaataaca catatcccta
    40741 ctggtgtagt agttcagtgc caaaatgaaa ggtctcagca tctaaataag gacagagcta
    40801 tgagactttt aatggcaaaa ttgatagaat taaaagagtt agaacaaaag gaaaaaatag
    40861 aagatataca aggcaaatac tcacaaataa cttggggtag tcaaattagg tcatatgttt
    40921 ttcaaccata taaattggtt aaagaccata gaacaaatgc tgagtttgga aatgtagata
    40981 gtgtaatgaa tgggaatata gacttgttta ttaatgaata cttaaaaatg aataaaatag
    41041 tttaataaat atgcactgta attaagatat atgtataaga aggttagtgc ttaatatgca
    41101 cttacagcac ttaggctgag gacgataacc ctcagccttc gttctgtttt gttatactta
    41161 tgaaaaacta acattaggag tgaataaatt ggatataaat caaattttaa aaaaagagtt
    41221 taatcttaga gatgaacaaa taaataatac attaaaactt attgatgaag gaaatacaat
    41281 accatttata gctagatata gaaaagaaat gactggcgaa atgagtgatg ttacactaag
    41341 agaattttat gaaaagttaa tgtatttgag aaatcttcaa agtagaaaag atgatgtagt
    41401 tagactaata gatgaacaag gtaaattaac agatgaaata acacaaaata tagaaaaagc
    41461 aaaaacacta caagaagtag aagatatata tgctccatat aaacaaaaga aaagaactag
    41521 agcaacaata gcaaaagaaa aaggattaga aaaattagca ttgtcaatac tagagaataa
    41581 tttagataat atagaaatag aggcaaagaa ttatcttgat gaagagaaag aagttctttc
    41641 tattgaagat gctctaaaag gtgctagaga tataattgca gaactagtct cagatgatgc
    41701 taagattaga aagtatataa gagaattagc tttaagagaa ggtatgatag ttagtaaaag
    41761 tgccactgat gagaaatcgg tttatgacat gtattatgat tatagtgaag cagtaaaaag
    41821 tatgcctcca catagagttt tggcaataaa cagaggagaa aaagaaaact tcctaaaggt
    41881 taagttagaa ataaataatg ataaagtttt aaattacata ataaacgaat atgtaaatga
    41941 taaaaatttc aaaaataaag aagaaatcgt aagttcaata gaagattctt ataaaagatt
    42001 aattttccca tcaatagaaa gagaaataag aaatcattta acagaaatag ctcaagaaag
    42061 agctataagt gtatttggaa agaatgttaa aagtttattg cttcaaccac cagttaaaga
    42121 caaagtagtt atgggatttg accctgcatt tagaactgga tgtaaaatag ctgtagtaga
    42181 taaaaatggt aaactactag attatacaac agtatatcca acagaccctc aaaatgatgt
    42241 agagggagca aagaaagttt taaaaggatt gattgaaaaa tacgatatag atataatttc
    42301 aattggaaat ggtactgcat ctagagaatc agaaactttt gtatcagaaa tgataaaaga
    42361 aatagataac gaagtgcagt atgtgatagt tagtgaagca ggagcatctg tttattcagc
    42421 ctctgaactt gcaaatgagg aacatccaga tataaatgta tcaataagag gagctatatc
    42481 tatagctaga agattacaag acccattagc agagcttgta aaaatagacc ctaaaagtat
    42541 tggcgttggt caatatcagc atgatttaaa caaaaagaga ttagaagaag tactagatgg
    42601 agttgttgaa gattctgtaa atagtgttgg agtagattta aatactgcat catactccct
    42661 actagaacat gttgcaggaa taagtaaaac tatagcaaaa aatataatag catatagaga
    42721 agaaaatgga gattttacgt caagagcaca attgaaaaaa gtaaagagac taggaccaca
    42781 agcatttact caatgtgctg gatttatgag aatcttagag ggtaaaaatc cactagataa
    42841 tacaggtgtg catcctgaaa gttatgatat atgtaaaaaa atgattgaga taattggata
    42901 ttctttagat gatgtaaaga ataagaatat atgtgaaata gatgaaaaaa taaaagaaat
    42961 tggcttgaga gaattaagtg aaaaactgga agtagggcaa gttacgttaa aagatatcat
    43021 aactgaaatc aaaaaaccag gaagagaccc aagagaagaa ggtataaaac ctatacttag
    43081 aactgatgtc ttaaaaattg aagatataca agagggtatg actttaaaag gtacaataag
    43141 aaatgttgta gattttggtg catttgtaga tattggaata aaaaatgacg gattagttca
    43201 taaatcagaa atgagtaata gttttgtgaa agaccctatg agtattgtta cagtaggtga
    43261 tatagtagat gtaaaagtca ttggaattga cttaaacaaa aaaagagttg ccttatcaat
    43321 gaaaaaatag ataagtttat gtatggttat agtacttaaa ttctatgttt gtaaatgagg
    43381 ttactcgagt gtataaatgt taaaaatttg taaaagtata cagtaagaaa atatattaaa
    43441 ataagaacga tagtcttgaa aatcaggagg aaaatgaatg atttttataa aaaatggtaa
    43501 aataaatacc ataacaaatg ggataattca tggagatata cttatagatg aaggaaaaat
    43561 aatagaaatt ggagaagatt tgatagctcc tttagatgta gaggtaatag atgctagtaa
    43621 taaacttgtg tttcctggat ttatagatgc tcatactcat ttaggtttgt gggaagatgg
    43681 tatagggttt gaaggagcag atggaaatga agagactgac ccaataacac cacaattaaa
    43741 tcccatagat ggaattaatc caatggatag aacttttaaa gaagcttttg aaggtggaat
    43801 aacttctgtt tgtacaaccc ctggaagtgc aaatgttatg ggtggtcaat gtatagctat
    43861 aaagacatgt ggaaaaagaa ttgataaaat ggtaataaaa aatccagttg catctaaaat
    43921 cgcttttgga gaaaatccaa agagctgtta tggtcaggat gataaatctc ctcaaactag
    43981 aatggctata gctgctttac ttagagaaaa cctaaaaaaa gcagaagaat atttagagga
    44041 tattgatatg tatgagagtc atgatgatga agactgtgaa aaacctgaat atgatataaa
    44101 aatggaaagt ttaataccag ttttaagaag agaaattcct tttaaggcac atgctcatag
    44161 agcagatgat atgtttacag caataagaat tgcaaaagaa tttaatttaa agcttacatt
    44221 agaccattgt acagaaggtc atcttattgt agatgaatta gtggaagaag ggtttcctgt
    44281 aatagtaggg ccatctttgt cagaaaggtc taaatttgag cttagaaatt tgacatttaa
    44341 tacagcagga atactttcaa atgcaggtct tgatgtatgt ataatgacag accatccagt
    44401 aataccagtt caatatcttc caatatgtgc aggtattgca gtcaaacatg gtatgaaaga
    44461 agaaaaagca atagaatcaa ttacgataaa tccagctaag actttaggaa tagaagatag
    44521 agtaggctct atagaagttg gtaaagatgc agacctagta atttgggata attctccact
    44581 agagattcaa agtaatgtac tttacactat aataaatggc aaagtagtgt atgagaaaaa
    44641 ataacttgac aattattaaa aaattaacta atataagagt gtaaggaaaa ttaaaaaatc
    44701 aaagaagatc ttcggggcag ggcgaaattc cctaccggcg gtatagcccg cgaactactt
    44761 aagtggttga tttggtgaga ttccaaagcc gacagtaaag tctggatgag agaagatatt
    44821 tatggaagat aaataagttt attttagttt gtttaaacta tatccataaa aactcgaaga
    44881 tagggtcttc gagttttttg tttttcctaa aaaatataaa catattgatt agggggaaat
    44941 aattatgcaa aacacagcca aaagaggcaa tatgatttca acaagaactt tagtagtaat
    45001 gtcaatttta tctgcaatat cttatttatt aatgtttatt caagcaccaa taccaggtat
    45061 atttccagat ttcttaaaaa ttgacttatc agatattcca gcaatattcg gaggaatgtc
    45121 tctaggacca tttgttggat ttggaatagt tgtagtaaaa aatgtgttac aagcaataac
    45181 agcaacaaca actggaggaa taggagaatt tgcaaatata ttaattggtg gttcttatgt
    45241 aatgatactt tgttattcat ataagagaag tggagattta aaaggtgtat tgattggttt
    45301 tgtattagga acaatatcta tgactataat tggttcatta gtaaattatt ttgtaatgtt
    45361 gccactttat ggtcaactta tgggactaga tgctataata ggtttaggtt cagctataaa
    45421 tcctcaagtt catgatttat tcacttttgt aatttggatg atagctccat ttaatgtgct
    45481 gaaggctgtt atactatctt tagtaacatt gccactttat aaaaaaatgg gaaatgtaat
    45541 aaagaaataa ttagacaaga aaatataaaa ttaaatgtat tgaatagcca taatttattt
    45601 ataacttcag tcctatattg tgattgatat attaaatata ttatggcttt attaatttat
    45661 caaaaaatgt atataaaata agaaatattt agacaaattt aaattttaaa ataaaggaag
    45721 aattaaaacg attaactatt aaaatatgaa aaaggtatct gcaatataca gagcgaattt
    45781 ataataaata aaaaataaag ttattattaa aaaaatataa agattagtaa tcaaattttt
    45841 aataataact aatttaataa tgatgggagt gtatattcat gaacttatct aaaaagataa
    45901 aaaaattatt tttagatgat aaagggtatt attttaatag taattggaaa gatattcata
    45961 ttggtattta taatacaaaa ttaggagatt ctttaaagta tatcactaat gaatatattg
    46021 aaaatctgga aagggtaaaa tatgactatc gtgaattaaa aaatatagaa aatcaaactg
    46081 atggtatgtg tattttagca ataaggcaga attacaaggc attagagtat gtaaaaaatc
    46141 aaaccgaaga attatgttta gaagcaataa agtataatta taaagcactt gaatatgtaa
    46201 aagagcaaac agaatatata tgtcttgagg ctataaaaaa ggattgtaat gcacttaagt
    46261 atgtacgaaa taaaacagaa aggcttatta taaaagtaat acgtcattct tccaatatag
    46321 atgttgtaag tatactaaag gctttagaga ctcaaacaag aagaatatgt ttagaagcaa
    46381 ttaaaaaaga tggcaggtgt cttgcatacg ttagagaaca gagtgaagaa ttatgtatag
    46441 aggccattaa acaaaattat aaagcattaa aatatgtaga aaatcaaacg gaaaaaatgt
    46501 gtatagaatc cgtaagacaa aatggtatgg cattacaata tgtaaataaa caaactgata
    46561 aaatatgtat agaagctgtc aaacaagatg gaagaagtct acaatttgtc aataataaga
    46621 cagaagaaat atgtataaat gctataagat atttaaataa aaaatataat attaaagatg
    46681 tactaagtta catagataag tatacagaag atatatgtat agagattgta agacaaaatg
    46741 gaaaaatgct tatgtatata aaaaatcaaa cagaaaaaat gtgtattgaa gcagtaaaag
    46801 agaactataa gtctcttaag tatgtaaaag agcaatctga gagaatatgt aaagaagcat
    46861 tgaagcaaaa ccataaagca aaagagtatg taaaaattgc tattgatgat tgtatataag
    46921 gtgtagatac agccatcaat agtgaaagat aaaaaaagat aaagacaagt actttataat
    46981 aatctaggct tatactataa ttaattaagc agtgatataa attcacatga cgattatatc
    47041 aaaatggaga tgaattgatg gcaaaaatat atttagaaaa tgaaaataaa actagagaaa
    47101 taggatataa attaggtaaa cttttaaaag aaggcagtgt tatctgttta gtaggagatt
    47161 taggagctgg taaaactaca atgacacaaa gcttggcaga ttcattaggg atagaagatt
    47221 atataacaag tcctactttt actataataa atgaatatga aggaaaaata cctttgtatc
    47281 attttgatgt ttatagaata ggtagtagtg acgaaatgta tgatattggt tatgatgaat
    47341 atgtaaattc aaatggaatc tgtataatag aatgggctaa tctaatagaa gatatacttc
    47401 caaaagaata tttgaatata gaattgagat ataaagatga cggaagagaa atgatattaa
    47461 ctccaaaagg agagttttat aaagagatag ttgaggagtt gattaaataa tgaaaatact
    47521 aggtatggat acgtcttcta tggcagcaag tgtagcagtt gttgaagatg ataacttaat
    47581 atgtgagttt acagtaaaca acaaaaaaac tcattcacaa aagcttatgc caatgataga
    47641 aaatatgttg agtatgagtg atttaagtat aaaagatatg gatttattag ctgtatgtat
    47701 aggtccaggc tcgtttactg gacttagaat aggtatggcc acagtaaaag ctatggctca
    47761 tgtaaataat ataccaataa tagcagtaaa ttctttggaa tctctagcaa ataacataaa
    47821 tttctgtgat agaaagatat gttgtatatt agatgcgcag aaaaatcaag tttattcagc
    47881 taaatacaag tttgaaaatg gagagatgat agaattagat ggtgtagatg ttgtagagtt
    47941 tgatgcttta gtagatgaaa ttgtatcaac taatgaagaa tttataatag ttggagaagc
    48001 tgtatacaaa tataaagata agttagagaa tataaaaaat ataaaaatac cttcaccagc
    48061 taataatgta tctaacgcag gaagcttatg ctctttagca ttaaataaat ataataaaaa
    48121 tatagatgta catacttgtt atacgataaa tcctatgtat atcagaaaat ctcaagctga
    48181 agtacaatat gatgaaaaaa tgaagaggct aaataatgga agataataca aaactaatag
    48241 tagatgatat aaaaatagaa gaaatgacta cagaagatat agatgaagtt tttgaagtag
    48301 aaaaaaattg ttttgaagat tattggtcaa aagagtcttt tagaaaagag ttgtctaatg
    48361 aagtagcaag gtatatagta gctaagttaa atggaaaaat tgtcggatat gtgggaattt
    48421 ggcttatact tgatgaaggg catataaata atgtggctgt tcatagtgac tatagaggga
    48481 aaaaaatagg ggacaagttg ataaaaggca tagttgattt atgtaaagat aataatattg
    48541 catctatgac attagaagtt agagcatcaa ataaaatagc acaaaattta tatagaaaat
    48601 atggatttaa aatgggtgga ataagaaaag aatactacag tgataataaa gaagatgcaa
    48661 ttataatgtg gaaccaatta aaagaggtgt agagtttatg agtgatataa taacattagc
    48721 tatagagagt agttgtgacg aaacagctgc atcagtttta aaaaatggta gagaagtact
    48781 ttcaaacata atctctactc aaatagagac acataaaaaa tttggtggtg tagttccaga
    48841 agtggcttca agaaaacatg tggaaaatat tgatatagtt gttcaagaag ctttagacaa
    48901 agcaaatata ggttttaatg atatagacca tatcgcagtt acatatggac ctggactagt
    48961 aggtgcttta ttggttggtc tatcttatgc taaagcactt gcatatactt tgaatattcc
    49021 acttgtagga gtaaatcata tagaagggca tctaagtgca aactatatag agcataagga
    49081 tttgaaacct ccttttataa cattaatagt atctggaggt catactcact tggttgaagt
    49141 aaaagattat ggcaagtatg aaattttagg aaaaacaaga gatgatgcat ctggagaagc
    49201 attcgataaa atttcaagag caatgaattt aggttatcca ggaggaccta ttattgataa
    49261 tctagccaag aagggaaata aacatgctat agagtttcca agagcctatt tagaagaaga
    49321 tagttatgat tttagtttta gtggattaaa atcttctgta ttgaattatc taaatgggaa
    49381 aagaatgaag aatgaagaaa tagtagttga agatgtagca gcatcttttc aagaggctgt
    49441 ggtagaggtt ttatctacta aggcactaaa agcagtaaaa gaaaaaggat acaatatcat
    49501 aactctttct ggtggagttg cttctaattc ggggcttaga gctaaaataa cagagcttgc
    49561 taaagacagt ggtattactg ttaagtatcc tcctctaatt ttatgcacag ataatgcagc
    49621 tatgataggg tgtgcaggat attataattt tataaacggg aaaacgcatg atatgagttt
    49681 aaatgcagtt ccaaatttaa agataaatca gtagagttag ataaatttgt aaatataaaa
    49741 gtattgtttc aaaaaagtaa gcagaagtga aattaaatat aataaaaatc aatatagtat
    49801 atattaaaac ttgaagtaaa actatttata atctttatat tccaatgaat tgcaacacat
    49861 tttaggatat acaggttgtc tttggcttag atttgcttca agtttaattt tttctataga
    49921 aagattattt taaaaagtgg gtaatatacc cttttttttc attcttaaaa agttttatac
    49981 taattataga aagattaagg aggggaagaa atgagtcaaa gtaaagaaga caaaataaga
    50041 agtattttgg aagcaaaaaa tatcaaatca aattttcaaa ataaagaaaa tttaagtgaa
    50101 tttaatgaaa aaaaagcaag taaaagagca gaagacttat tagatgtata ttataataca
    50161 ttatctactg cagatatgga atttccatat tggtataata gagaatatag aaaatcagat
    50221 ggtgatatac cagtagttag aagagctaaa gctcttaaat ctgctttttc acatatgaca
    50281 ccaaatataa tacctggtga gaaaatagta atgcaaaaga cacgccatta tagaggttct
    50341 tttccaatgc catgggtaag tgaaagtttc tttgttgctc aaggagaaca aatgagagaa
    50401 gaggcaaaga aattagccag taacacagct gatgaactta caaaatttgg gtctggcgga
    50461 ggaaatgtta ctgaaagttt tggaaatgtc gtttcaatag ctggtaaatt cggaatgaga
    50521 aaagaagaag tacctgtact tgtaaaaatg gcaaaagaat gggttggaaa gtctgttgaa
    50581 gatttaggtt ttcactatga aaaaatgatg ccagattatg atttaaaaga aaacctaatg
    50641 agtacactta tatgtatgtt tgattcagga tatacacttc ctcaaggtag agaagttata
    50701 aattattttt atcctttaaa ttatggatta gatggaatta tagaaatggc aaaagaatgt
    50761 aaaaaggcag ttgcaggaaa tgcatctgga gatgggctta ttggtatgga ccgtctatat
    50821 ttttatgagg ctgttattca agttattgaa ggattacaaa cttggatttt aaattatgca
    50881 aaacatgcaa aatatttaga aagtatagag acagatttag aagctaaaaa agaatactca
    50941 gatttagtgg agatattaga acatattgca cataaacaac ctcgtacatt tagggaagct
    51001 cttcagttga cttatacaat tcatatagca tcagtaaatg aggatgctat atctggtatg
    51061 tctataggcc gttttggtca aattttatat ccttggtatg aacaagatat agaaaaagga
    51121 ttaattacaa aagaagaggt tatcgaatta ttagagcttt atagaataaa aatcacttgt
    51181 attgactgtt ttgcttcagc gggcgtaaat ggaggagtac tatcaggaaa tacatttaat
    51241 actttatcta taggtggatt aaaagaagat ggttctacag gagctaatga actagaggag
    51301 ttattattag aagcaagtat gagatgtaga actcctcaac ctagtttaac tatgttatat
    51361 gatgaaaaac ttccagaaga tttcttaatg aaagctgctg aatgtacaaa gcttggttct
    51421 ggttatccag cctgggtaaa caactcaaat ggaacaacat ttatgatgaa acaatttgct
    51481 gatgaaggta tgactgttga agaagcgaga gcatttgcat taggaggatg tttggaaacg
    51541 tctccagggt gctggaaaca gttaacttta aacgggaaga catactctat tgcaggtgga
    51601 gcaggtcaat ctgctggctc tggagttcac tttatagcta atcccaaaat tttagaactt
    51661 gttcttatga atggtaaaga ccatcgtatg aatatacaag tatttgagcc tcataataag
    51721 ccattagata cttatgaaga agtcatagaa gtatttaaag attattataa acaagcaata
    51781 aatgttttag aaagagcaaa taacattgag ttagatatat ggagaaaatt tgatacttct
    51841 ataataaatt cactattaaa gccagactgt ttagacaaag gtcaacatat aggaaatatg
    51901 ggatatcgtt ataatgcaac attaaacgta gaaacttgtg gtactgttac tatggtcaat
    51961 tcatttgcag cacttaagaa acttgtatat gatgacaaag catttacaat agaggaaatg
    52021 aaggatgcca tattaaataa ttttggattt aaagatgcat tagaagtagg aaattattct
    52081 atggcagacc aagtaaaagt agataagact ggtaaatatg atgctattta taaggcctgt
    52141 cttgatgcac ctaaatatgg aaataatgat ttgtatgcag ataatattct taaaaactat
    52201 gaggtatggt tatcaaaagt atgtgaagaa gcacaatctt tatatgctaa gaagatgtac
    52261 ccttgccaaa tatctgtttc tacacatgga ccacagggag ctgctacctt ggctactgca
    52321 gatggtagac taagtggaac aacatattca gatggttcag tctctgcata tgcaggaaca
    52381 gataaaaatg gggtatatgc attatttgaa tctgcaacta tttgggacca agcagtagtt
    52441 caaaattctc agatgaattt aaaacttcat ccaacaacta taaaagggca acaaggaact
    52501 aaaaaattat tagatttaac aagaagttat ttaagaaaag gtgggttcca tattcaatat
    52561 aatgttgtag attcagaaac attaaaagat gcacaaaaaa atcctgacaa ttatcgtcaa
    52621 ttaatggtac gtgttgctgg gtttactcaa tattggtgtg aattaggtaa accaatacaa
    52681 gatgaagtaa ttgctagaac agagtatgaa ggggtgtaaa atatgagaaa gcatagtgat
    52741 tgtatgaatt tttgtgcagt agatgcaacc aaaggaattt gtagattatc aaaacaaatg
    52801 attaatttag atgatgcagc atgtccagag ataaaagtaa tgccaaaatg taaaaattgt
    52861 aaaaattttg ttgaagctaa tgatgaaggt ataggcaaat gtgttggcct agagaaagaa
    52921 gactgggtat attcaacatt gaatgcaatt acttgtgaag gacatgtgtt taatgagtag
    52981 tcaaaagcaa ttagaaggca tgattttcga tgtacaaagc ttttcagttc atgatggtcc
    53041 aggttgtaga acaactgtat ttttaaatgg atgtccactg agttgtaaat ggtgtgcaaa
    53101 tccagaaagt tggactgtaa gaccacatat gatgtttagt gaattatctt gtcaatatga
    53161 aaatggatgt actgtatgtc atggtaagtg taaaaatggt gcattgagct ttaatcttga
    53221 taataagcca gttatagatt ggaacatctg taaggactgt gaaagttttg aatgcgtcaa
    53281 ctcatgttat tataatgcgt ttaaattatg tgcaaaacct tacactgtag atgaacttgt
    53341 gcaggtaatt aaacgtgatt ctaacaattg gagaagtaat ggaggagtaa catttagtgg
    53401 aggtgagcca ctacttcagc atgaattttt acatgaagta ttattaaagt gtcatgaggt
    53461 aaatatacac actgccattg aaacgagtgc atgtgtatca aatgaagttt ttaataaaat
    53521 atttaacgat attgattttg catttattga tataaagcat atggatagag aaaaacacaa
    53581 agaacaaaca ggtgtgtata atgatttaat attagaaaat atatcaaacc ttgcaaattc
    53641 tgattggaat ggaagattag tacttagagt accagttatc tctgggttta atgatagtga
    53701 tgaaaatata agtgatatta tatcttttat gcacaaaaat aatttggtag aaattaatct
    53761 attaccattt cacagattag gagaatcaaa atggattcaa ttaggcaagg aatatgagta
    53821 ttctgataag ggtgatgttg atgaaggtca tcttggagaa ttacaagata tatttttgga
    53881 taatggtata gcttgttatg ttggtcatga gacagctttc taaaattaaa tacaagtttt
    53941 aattaaaaaa ggatgttaca ctaaaagcta gagttacatc cttttttatg tggtttttat
    54001 gtagaaataa ttaagtaaag ttttatatat tttatatatt gtttatattg ctttatgtcc
    54061 ataaatattg ctttaaatat actttatata ttttttatag tttaaattac agttagagaa
    54121 tatattacgt ttactttatt gaagcagaag ttttgtggta aaatactact atgaaaaaat
    54181 ttaatagaat atattttaga aacaactaaa aataatagtt aggaggaaga catctatatg
    54241 aacaaatcat ggagcaactt gattattaag aataaagaca aaaaagtacg ttcagggtgg
    54301 attattatag ctgttatggc taccttttac atatttcagt attatttaag catgatactt
    54361 gtggaaataa taagaaaaat cctggttgct acaggggaca ttaatttaaa aacaaactat
    54421 ttttcagaat atgctaattg gttaaatgat gttgggttac agattgtatt tcaaattcta
    54481 acagattcac ttatgattat tattcctata attgtatgga gatttattat gaaaaatcct
    54541 attagtgaaa tgggattaca ttcttataga attaagaaaa aagaaggata tgtaggaatg
    54601 ttattaggaa tatttaattg tacagtaatc tttattataa caataacaat tggtggtggt
    54661 catgttactt catgggtacc aaaaatttca cctttaacag tttcttgaat acttgtcttt
    54721 attttagttg cttttgcaga agaaatttta aatcgtggtt tttttatggc agttttacga
    54781 agatgtaaaa atatttattt tataatgatt gttccatctg ttatttttgg gttgattcat
    54841 atatggaatc ctgatgttac ttttttgtct gtaattaata ttataattat tgggattctt
    54901 ttttcatata tgtttataaa gtcgtcgaat atatggatgt gtataggtta tcattttaca
    54961 tggaatgtat ttcaggggat tatatatggt atgcctgtaa gtggacttca ggttccaggg
    55021 attataacta cacaaatcac tcatggtaat ctattaaatg gtggtatgtt tggtattgaa
    55081 ggtggaattt tgactacttt tgttaatcta cttagtttta tatttgtatg gtattactat
    55141 agagattcta agtatgattt tgttagtgat aaagtaaatg tgaccaatat caagaattag
    55201 ttgattataa taaattttaa taatatcttc taagcttaaa gtattctgta ggattgttgg
    55261 aaatttacaa aaatataaaa ataacttgga tattctattt actccaagtt atttttatat
    55321 tcttttgtaa atagaaatgg aacataaaaa attaaaacaa ccatagatac tattagtata
    55381 tcatggtaag tgatagttga ttctttagaa tttgtgataa gaaaagcact attatttaaa
    55441 atatgaatta tagcacaaaa ccagatgttc ttagttttta tataaagata tcctaaaaat
    55501 attgaaattc caactatatg tatcgaacgt aatattaatc ctaatattgg tgtttcaggt
    55561 gtatataatg taaatattag tggtaagtgc cacaaactcc aacaaatacc taatattata
    55621 actcctagtt ttttaccaaa tttattaaaa aaaatattct gtaaaaaacc tctccaggca
    55681 tattcttcac taaaaaaaat ataaatagta gttaatgatg ttattggagt aaatataaat
    55741 tttatgatac cactataatt aggttgagac ccttgtagta ttaatataaa tgatgttgga
    55801 acaattacag aaatcataaa gtaaataatt aacaaagaac ctagtttaat attttttata
    55861 ggatataaat tttttttatt tgatgaatat attataattg ataaactact tataaaagtt
    55921 aatattaggt ttataattat aaattcatta ttatttatta gatttaataa cttaagaaca
    55981 attaataacg aataagtaag aaaaaatagt atgattagtg aataaaattc atacatatca
    56041 ttatttaatc cttcagtata aaattttgca atagcaactg aagatagagg taatatcatc
    56101 atgaatgatg caaagttttc tggatcaata aatttattat aatacaataa taaacctaat
    56161 gaataagt
//
LOCUS       DBGJFU010000011        55678 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.011, whole genome shotgun sequence.
ACCESSION   DBGJFU010000011 DBGJFU010000000
VERSION     DBGJFU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.011"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(80..634)
                     /locus_tag="ACOIAS_000661"
     CDS             complement(80..634)
                     /locus_tag="ACOIAS_000661"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGZ2700382.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(712..1077)
                     /locus_tag="ACOIAS_000662"
     CDS             complement(712..1077)
                     /locus_tag="ACOIAS_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2700383.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            1308..1442
                     /locus_tag="ACOIAS_000663"
     CDS             1308..1442
                     /locus_tag="ACOIAS_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700384.1"
                     /translation="MNISRKAMKIIELAQKIANKRGISVEEAWSEAVTEYKNKYEHIA
                     "
     gene            1542..2327
                     /locus_tag="ACOIAS_000664"
     CDS             1542..2327
                     /locus_tag="ACOIAS_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2700385.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(2424..3248)
                     /gene="pdaA"
                     /locus_tag="ACOIAS_000665"
     CDS             complement(2424..3248)
                     /gene="pdaA"
                     /locus_tag="ACOIAS_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HGZ2700386.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            3469..6588
                     /locus_tag="ACOIAS_000666"
     CDS             3469..6588
                     /locus_tag="ACOIAS_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HGZ2700387.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            6606..7205
                     /locus_tag="ACOIAS_000667"
     CDS             6606..7205
                     /locus_tag="ACOIAS_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HGZ2700388.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(7462..9591)
                     /locus_tag="ACOIAS_000668"
     CDS             complement(7462..9591)
                     /locus_tag="ACOIAS_000668"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HGZ2700389.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            9815..10105
                     /locus_tag="ACOIAS_000669"
     CDS             9815..10105
                     /locus_tag="ACOIAS_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700390.1"
                     /translation="MSGLKKILRILVISMMICFAMTFALVLINSVMGVHIGLDYYFLM
                     GSVLTVCLTIGIVLCNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            10271..11662
                     /locus_tag="ACOIAS_000670"
     CDS             10271..11662
                     /locus_tag="ACOIAS_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HGZ2700391.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            11777..13246
                     /locus_tag="ACOIAS_000671"
     CDS             11777..13246
                     /locus_tag="ACOIAS_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HGZ2700392.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            13311..13904
                     /locus_tag="ACOIAS_000672"
     CDS             13311..13904
                     /locus_tag="ACOIAS_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700393.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            14035..15432
                     /locus_tag="ACOIAS_000673"
     CDS             14035..15432
                     /locus_tag="ACOIAS_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGZ2700394.1"
                     /translation="MEVSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEED
                     GDETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKN
                     YITSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKY
                     VNLISSEDFKTFLGRYNHSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDK
                     LGNVKKREGYLDKLKELDKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCV
                     LIGMGIYLIQSKKRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEP
                     EREITVADEEDLYYQALNLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKL
                     LGNKDEAIKYYKKYINTYTKNDQYYDDSYYELGMFYYDNGDLKNAQQTFYSLRSEVPD
                     SMYNNSKIKEILSEK"
     gene            15614..15985
                     /locus_tag="ACOIAS_000674"
     CDS             15614..15985
                     /locus_tag="ACOIAS_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGZ2700395.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            15978..16667
                     /locus_tag="ACOIAS_000675"
     CDS             15978..16667
                     /locus_tag="ACOIAS_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HGZ2700396.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            17187..18053
                     /locus_tag="ACOIAS_000676"
     CDS             17187..18053
                     /locus_tag="ACOIAS_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2700397.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            18295..19104
                     /locus_tag="ACOIAS_000677"
     CDS             18295..19104
                     /locus_tag="ACOIAS_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGZ2700398.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            19248..20312
                     /locus_tag="ACOIAS_000678"
     CDS             19248..20312
                     /locus_tag="ACOIAS_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HGZ2700399.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            20340..21059
                     /locus_tag="ACOIAS_000679"
     CDS             20340..21059
                     /locus_tag="ACOIAS_000679"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2700400.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            21083..21667
                     /locus_tag="ACOIAS_000680"
     CDS             21083..21667
                     /locus_tag="ACOIAS_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HGZ2700401.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            21669..23018
                     /gene="pabB"
                     /locus_tag="ACOIAS_000681"
     CDS             21669..23018
                     /gene="pabB"
                     /locus_tag="ACOIAS_000681"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HGZ2700402.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            23008..23751
                     /locus_tag="ACOIAS_000682"
     CDS             23008..23751
                     /locus_tag="ACOIAS_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HGZ2700403.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            23785..24486
                     /locus_tag="ACOIAS_000683"
     CDS             23785..24486
                     /locus_tag="ACOIAS_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700404.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            24512..25084
                     /gene="folE"
                     /locus_tag="ACOIAS_000684"
     CDS             24512..25084
                     /gene="folE"
                     /locus_tag="ACOIAS_000684"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGZ2700405.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            25148..25939
                     /gene="folP"
                     /locus_tag="ACOIAS_000685"
     CDS             25148..25939
                     /gene="folP"
                     /locus_tag="ACOIAS_000685"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGZ2700406.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            25950..26315
                     /gene="folB"
                     /locus_tag="ACOIAS_000686"
     CDS             25950..26315
                     /gene="folB"
                     /locus_tag="ACOIAS_000686"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HGZ2700407.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            26308..26814
                     /gene="folK"
                     /locus_tag="ACOIAS_000687"
     CDS             26308..26814
                     /gene="folK"
                     /locus_tag="ACOIAS_000687"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGZ2700408.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            26795..27580
                     /gene="aroF"
                     /locus_tag="ACOIAS_000688"
     CDS             26795..27580
                     /gene="aroF"
                     /locus_tag="ACOIAS_000688"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGZ2700409.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            27835..29625
                     /gene="dnaG"
                     /locus_tag="ACOIAS_000689"
     CDS             27835..29625
                     /gene="dnaG"
                     /locus_tag="ACOIAS_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGZ2700410.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            29643..30809
                     /gene="rpoD"
                     /locus_tag="ACOIAS_000690"
     CDS             29643..30809
                     /gene="rpoD"
                     /locus_tag="ACOIAS_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGZ2700411.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            30974..31663
                     /locus_tag="ACOIAS_000691"
     CDS             30974..31663
                     /locus_tag="ACOIAS_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HGZ2700412.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            31677..32774
                     /locus_tag="ACOIAS_000692"
     CDS             31677..32774
                     /locus_tag="ACOIAS_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGZ2700413.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            32837..33409
                     /locus_tag="ACOIAS_000693"
     CDS             32837..33409
                     /locus_tag="ACOIAS_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2700414.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(33469..33936)
                     /locus_tag="ACOIAS_000694"
     CDS             complement(33469..33936)
                     /locus_tag="ACOIAS_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HGZ2700415.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(34322..34798)
                     /locus_tag="ACOIAS_000695"
     CDS             complement(34322..34798)
                     /locus_tag="ACOIAS_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGZ2700416.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(34918..36009)
                     /locus_tag="ACOIAS_000696"
     CDS             complement(34918..36009)
                     /locus_tag="ACOIAS_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HGZ2700417.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(36002..36391)
                     /locus_tag="ACOIAS_000697"
     CDS             complement(36002..36391)
                     /locus_tag="ACOIAS_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2700418.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            36583..37029
                     /locus_tag="ACOIAS_000698"
     CDS             36583..37029
                     /locus_tag="ACOIAS_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700419.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            37314..38018
                     /locus_tag="ACOIAS_000699"
     CDS             37314..38018
                     /locus_tag="ACOIAS_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700420.1"
                     /translation="MLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGIGYKWIGSENSVSI"
     gene            38005..38712
                     /locus_tag="ACOIAS_000700"
     CDS             38005..38712
                     /locus_tag="ACOIAS_000700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700421.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKN"
     gene            38731..39249
                     /locus_tag="ACOIAS_000701"
     CDS             38731..39249
                     /locus_tag="ACOIAS_000701"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2700422.1"
                     /translation="MIKSMLKLSKVEAKVINFKKDKVKFSELIHRAMLSMKIPMEIKN
                     QKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKENPLFSELIIKD
                     EGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIYVNSEKGQGTE
                     FHIIFHKMYDSD"
     gene            39329..40006
                     /locus_tag="ACOIAS_000702"
     CDS             39329..40006
                     /locus_tag="ACOIAS_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700423.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            40007..42568
                     /locus_tag="ACOIAS_000703"
     CDS             40007..42568
                     /locus_tag="ACOIAS_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2700424.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            42759..44033
                     /locus_tag="ACOIAS_000704"
     CDS             42759..44033
                     /locus_tag="ACOIAS_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HGZ2700425.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(44194..47235)
                     /locus_tag="ACOIAS_000705"
     CDS             complement(44194..47235)
                     /locus_tag="ACOIAS_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HGZ2700426.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            47442..47741
                     /locus_tag="ACOIAS_000706"
     CDS             47442..47741
                     /locus_tag="ACOIAS_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGZ2700427.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(47979..50207)
                     /locus_tag="ACOIAS_000707"
     CDS             complement(47979..50207)
                     /locus_tag="ACOIAS_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HGZ2700428.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            50500..52275
                     /locus_tag="ACOIAS_000708"
     CDS             50500..52275
                     /locus_tag="ACOIAS_000708"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700429.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
     gene            52288..54111
                     /locus_tag="ACOIAS_000709"
     CDS             52288..54111
                     /locus_tag="ACOIAS_000709"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700430.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            54229..54774
                     /locus_tag="ACOIAS_000710"
     CDS             54229..54774
                     /locus_tag="ACOIAS_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HGZ2700431.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            55034..55642
                     /locus_tag="ACOIAS_000711"
     CDS             55034..55642
                     /locus_tag="ACOIAS_000711"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGZ2700432.1"
                     /translation="MVDNLIKYIHKSYVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
BASE COUNT        22176 a         5879 c         9241 g        18382 t
ORIGIN      
        1 atagaaagca tctaatttta aatgattaaa gagcaagctt aaataaatca aacttgctct
       61 tttaacatag tgtatgtttt tatttgtgat agttagataa aaatttatct gtgtttttta
      121 catctgcaaa actgttctgt agtgatgcca tgaatacttg atgtacagta tttgcattaa
      181 tttctatatt attccaagat aaattcttcg ttgtacatgc atcgcttatt agagttatgt
      241 cgtatcgaag tttctttgct gttcttacac ttgtatcaat acacatatgg ctcatcattc
      301 cacagattac tagattagta atattttttt tctcaaggat actttgcaaa tctgtttcaa
      361 agaaactatc tggtgtgtgt ttagttataa tttcttcatt atctagtgga aaaacaattt
      421 tatttatttc tgaaccatga gtattaggaa gaaaaaaaga agctgtttta tctgttgata
      481 tatgctgtat atgaataact ggtaaattgt tttttctaaa aaacagtagt atttttttag
      541 cattttcagc agtttcttct gattgaaata attcacattt tccattttta aaataatcat
      601 tttgaatatc aatcaatatt aaagctatag acatgattat acctcctatt tatttttatt
      661 ataatactat tgtaaaaaat gtaaattact tactttttgg tatgtatagg attacttatt
      721 gtagcaaatt cctttattat ctagaatttc ttgttgacca tgttcaagca tatattcgat
      781 tcctatttct tgcattattt ccactgcttt taacattttt ttaccacgat tagtaagaaa
      841 atattcaaca tgtaatggat atccagcaga acttatttta tcaataatcc caaaatcacg
      901 taactctttt aattgttgaa tcaacatttt ttgatttatt ccataaatat tatgttgcaa
      961 ttcagaaaat gttttattag attgtcttaa ttcaaatata aggatagttt tccattttcc
     1021 tttgattatg tcatgtacaa tttcaagaag acatgtatat tcagctctta ttttcatagc
     1081 atatcacccc cagtaaaatg ataataaatt atatcacttg tcttgtgata tgacaaatat
     1141 atatgaactg tatataaatt ttatgtttta ttaagttcta aatttcttct attgtagttc
     1201 atatttagta cataaataat tagtaagata taagtaaata aatatataac atgactatag
     1261 aaatttaact ttaaaataac ttatgataat ttagaggagg aatatatatg aatatatcaa
     1321 gaaaagctat gaaaataata gaattagcac aaaaaattgc aaataagaga ggtatttcag
     1381 tagaagaagc ttggagtgaa gcagtaacag aatataaaaa taaatacgaa catattgcat
     1441 agatatttaa aaatttagaa tatttggtag aatataatga ttttaaatga tactataaat
     1501 tataaggtat atcattatta acatagaagg aggatatctg cttgattgta aaaatagata
     1561 atactttaga aaaagaattt tggcaatatg tatcacatga agaaagttta aatttattca
     1621 taattggata cgttgagaat tatggattta gctctcaata tcaagatata tggtctcagg
     1681 tagaagatgg aaatataacc tctataatat taaaaaataa gtcaacactt ataatttata
     1741 gttttaaaaa taattttaat ataggagaaa tgaagaatca tataaaagat ttagatgtag
     1801 aaagcataag tggaaaaaaa tgtgtaatag atagactaat aagtaaatac aaagattttt
     1861 atgaaaaact agataataaa ttttgtgtat taaaagaaat aaaagaaata gatttttcca
     1921 acatgaagga gtataaaata gaaaatgctc aggaaaaaga tattgatgaa ataggaaaac
     1981 ttttaaatag gtcagactat aaagtaagta aaaattacat agaagaaaga aaagttcatt
     2041 taaaagaggg aaatgtaaga gcatatttta ttagaaatga tgatactatg attagcactg
     2101 tgtcaactgg tatggaaact agttttttgg caatggtggt ctctgtaagt acagataaaa
     2161 gatatagggg aaaaggactt gcaagttata tggtttacaa tttgagtaaa gaattgttgt
     2221 tagaaggaaa agttccatgt cttttttata ataatgatgt agcaggtaaa atatatcata
     2281 atataggata caaagagata aatgaatgga caattctttt taaatagcta taaaaaataa
     2341 aaaactctat cgagaattaa agtatttata aataactata tttttactat aattcatgat
     2401 agagtttttt taaatttatg atactatttt ttattattta aataatcaag tgttttaagt
     2461 ttataacctt ctttttccca atgtgtaaga acctcatcta atatctcagt atttgttgat
     2521 gaatttggat gcagtaaaac tattgcacca ggatgagttc ttgaataaat tttgtttttg
     2581 gcaaattcat gagtaggttg tttcttttca taccaatcta cataagcaaa actccaaaat
     2641 attgatttat atcctaaatc ttgtgtatat tttaaagatt gttcgctaaa tttacccatt
     2701 ggaggtctaa aatacttagg catttctttt ccagtaacat ctttgtatgc tttttctaca
     2761 cttgtaattt cttctttgaa tttttcaaaa tctgtaatac ctgccattga tggatggctt
     2821 tttgaatgat tacaaactaa atgtccttct ttttccatac gctttattaa ctctggattg
     2881 ctttttatat aactttcaac tacgaaaaat tgagctttag cattgtgttt tttaagcgta
     2941 tctaataatt ttggagtgtt tccactttca tatccagcat caaatgatag atatataact
     3001 ttttcattgg gatttcctac ataataagct ccgtattttt taaaaaaatc agcttctttt
     3061 ataggtgatg gctgtttacc atcctcccta gggttaaaat accaatcata ttcatgtgtg
     3121 tcgagtgttg gactagaaat ttgagttttt gttttatcta atgatttaaa atttatacta
     3181 gcaattccaa aaagtattag agcaaatgct cctatcataa tgtatttttt taggctatct
     3241 tttttcacaa tttcacctct tatttaaaat ttaaagtata tttcttataa tatagtttga
     3301 atatttatag ttaaaaatat tcacatatac aaataataaa tgaaagaagt ttactgtaag
     3361 atataaagtt gatattaatt atcttaaata aagtgtatat aaatgataaa atataaagag
     3421 aaatgattgt aaataagtta ggctggttta acttgaaggg atataaaaat ggataatata
     3481 ataagtgtat tgaatgatgt tgttttttta gatatagaag ttagtggatt agattgttta
     3541 aattcagaaa ttttagaagt tggagctgtc aaggttaaag actggaaaat atatacctat
     3601 gaatcattaa taaaaaacaa atttgaggta cctgtagaag tattctcagt atgtaaaaat
     3661 ttagataaaa atgatttaga aatagctaat gaaatagaac tggtggaaga tagacttgta
     3721 aattttgtag aagatagttt cattatatgt catgatttaa gtctgaaaaa aaagtttctt
     3781 gagtatcata tgccaaagtt aaaaaacaaa tttattgatt taattgaatt agcagttatt
     3841 ttagaaccat atcataaaga ttatagctta gaatacttaa aaaatacttt aacaaattgt
     3901 aattctaaag tggaaaatag agctttatca gatgctattg atataataaa tattgtaaat
     3961 tgccttttag taaaatttaa taactatgaa aaaactactt tggagccatt gagttttaaa
     4021 ataaattcat atcttaaaaa atttaatctt ccaacatggg aatggagcaa atttttagaa
     4081 gaggcaaatt atgatttatc aaataatata aatataaaaa aagaatataa tatatttgac
     4141 tcaaaagaag aaaagaagaa ggaaagagaa accttaaaaa ttttaaatga agaagaaaaa
     4201 aattatgaag aattattaaa atacaaaact atttgggaga ataaagaagg atttacatat
     4261 gaatatagac ctggtcaata tgagcttact aaaacaataa gagagttgtt tagaaatagc
     4321 gaagatgaag aaaaaattgc atgtatagag gcaccaactg gtataggtaa aagtgtagga
     4381 tatttattgc ctgcaatatt agaggctaga attaataaaa aaagattatt aatttctact
     4441 gacacaaagg aattgcagat acaattaata aataaagata ttccaaatgt gttagattct
     4501 ttgggtctaa atggaaaagt aagttacgga tatataaagg gaaaaaataa ctatatttgt
     4561 atagatagac tggaagcata tatagatgat tatgagtctc aaaaccctac taaaggagaa
     4621 attttatctt taatattttt gaagagatta gttgaaggtg gcaagtatgg agatatagaa
     4681 gaaataaatt atttagtgtt tgacaacttc aaggaaataa gtactcactt aagaaatgta
     4741 agttgtgacc caaatatgtg cagacctaaa aaatgcaaaa aagattgttt atataaaaac
     4801 agaatagaag aattaaaaga agaacatata acagtagtaa atcattcact attagctaaa
     4861 tggccatata aggatgaaaa acctttagaa aatataatag tagatgaagc acataatttg
     4921 actgaaaaag gatatgattt cttttcaagc attataaatt caaagtcttt aaggtattta
     4981 ttacaagaaa tttatcctta cgagtttatt caaaatagta gctttatata taaaaaatat
     5041 agtagaaata tgagaaaaat aaaggctttt gataaatttt ataatgtatt aaaaattgga
     5101 agagaagata aacaaaaaat tgctagaagt ataaatctca ttattgaaga gatagattca
     5161 atattaaagt ttggtaattg taatgaatat aataatgtaa gtaattataa tttaagatgg
     5221 gaactgaatt tgcaaataga cgaaattgtg ggaaaactaa aaaaggatgg tatagatact
     5281 gaaataagtt atagagccta tagtgaaaag ataaagttga gttgtgagaa aataataaaa
     5341 aatttagttt caataataat aataatttat aggaatattg atgatgatag tatagataaa
     5401 gaagcagata tatataaatt tggaaaggca aagacaagag atttagagga tataaagatt
     5461 atatttgaaa tctttttaga gtatgatgaa aaggatgatt atgcaaggat agttgaaatt
     5521 gataagaatt ataatgactt tgaatttcga gtagtgccat taaaaatagc agatttattt
     5581 gaagaaaata ttttatctca attagaaaaa ggaatatttt tatcagcaac tttgagttta
     5641 tctgaaagta tgtcttattt taaaaataca ttaggaatag acagagttaa gaatgtggaa
     5701 aagattatag aaccaatttt tgattataaa aatagagtct ctgtagttgg attttctgat
     5761 atttgtgagt acagaaattc tgaatttcca aatgagatga gtaaaattat atctaatata
     5821 tcaaagatta cagaaggtca tacattagct ctttttaata gtaaagatag acaagaaaaa
     5881 acttatgaaa tattgaaaaa atacttacat agttttaact tagaaattta tgctgataaa
     5941 aaggggatta gacaccttaa cgatttaaat agaaaatgtg tagtacttgg ctcaaaagga
     6001 tgttttgaag gcgtagacat tccaggggat ggtttggttt gtgtaacttt agacaagctt
     6061 ccgaatctaa atccaaaaga ccctttgtat tttactataa tgaagaagta tggtatagac
     6121 tactatacta taaattatcc tcaaatgacc ataaaggtaa agcaagctat ggggagaatt
     6181 ttaagaagca aatatgacta tggatgtttt gtgatttttg atgtaggaac taatataagt
     6241 gtgttaaaga gattagagaa agatttacac gattgtaaaa tttcaaaggt taatagcaat
     6301 gagttttata cttatataag gcgacattta aataaatata gaagtttaat actaaaatct
     6361 gttatatttg atactataaa agcgttgaat gttgatgcta aaatggataa caatgatgtt
     6421 gataaggata ttataaaaaa agatataaat gaaaatataa gacaaagagc tgtaaaagga
     6481 gaagtgtacc atattgatat tataaaaaaa gatatgaaag taaaatattt tgatagaaac
     6541 tatcttataa atttagatat atttatgaga gaagaagata aaaattaaga gtgggagtga
     6601 tatatttggc aaaacaaaag ttttatgctg tgaaaaaagg taaaaatata ggtgtatata
     6661 atacatggga tgagtgcaaa aaacaagtaa atggattctc aggagctgaa tataagagtt
     6721 tttctacttt tcaagaagct aaagaatata ttgatgggtc agaaaaattg agttttcaag
     6781 aagataagga gtttatagaa gcatatgtag atggaagtta tgaacattca gtaaaaatgt
     6841 atggttcggg tgttgtgatt ttaaaaaata atgaagttat aaaaacttat agtgaaaaag
     6901 gaaaagagaa aactttagta agtatgagaa atgtagctgg agagatagag gcttctaaaa
     6961 ttgcaatgca gtactgtatt gacaataatg ttcaaaactt gattttatac tttgactatg
     7021 aagggattga aaagtggtgt actggagttt ggaaaacaaa taaagaagga actatagcat
     7081 ataaaaattt ctatgacagt attaagaata agcttaatgt aaaatttaca aaggttaaag
     7141 ctcattctgg aaataagtat aatgaggaag cagataaatt agctaaaaaa gctattggtg
     7201 tataataaaa aaatatttat acataattac atggtataag agtatatcta aattcctact
     7261 ttataattaa acaacttgta attaaacaac ttgtaattaa acaacttata attaaataaa
     7321 ttttgattaa aaaagcagat tgtattatga aaaaataagt atctaattta taaacaatta
     7381 ataatagtat taattagata caacttattt aatataataa aatctgcttt ttatataact
     7441 ataaaatttt cctacattct attagaattg cccataaata ccttcaagtt cttcttttaa
     7501 tacttcaggg tcatttatac caatagacat ggttctagcc agtatatttg cttggtcata
     7561 agttgatgtc catgagaata caccacctag acaatttcta agaacatatt gagtttttaa
     7621 gaatattgat aaagcatcat catatgacat tgcaaaatct ccatccttaa ctaaatatgg
     7681 aacttgagca gtattatcaa atctatattc atatccattt ttatttatat aatcccttct
     7741 aagctcatca taagttcttg ttatagtagc acctaatctt ccataaaatg gtataccaag
     7801 aaggattttt tcagaaggca ttccagcatt ctcaagattt cttaccattg catcaacact
     7861 atatcctggc aaagataagt ctgaatcaaa aagatttgct tgatgttttc taccatttgg
     7921 gcctgtttca cctgctgtaa aatcataact cataagatta aaataatcta ttataggagc
     7981 tattttatct atttcagcac ttgaatttat atatcctcta tctcctgttc cagctacact
     8041 aagccattta tcatctccta taacatctct tatggcagtt agtaagagtg taaagttttc
     8101 tctatcttga ggtcttgatg ttattccaga tgcactactt ccaggatatt cccagtctat
     8161 atctattcca tctaaagcat attcatttat catctgattg acctgtcttg caaaattata
     8221 tctagatgta ggtgttaatg ctgcatcaga gaatccttca gctccccaac ctccaatagc
     8281 tacaattact tttaagctag gtttttcacc tttaagagca agtaattgat ttaaaaatct
     8341 aggataagga ataaacaaat ttccagactg gtctatctca gcaaaagcaa atataactgc
     8401 atctaatagt tgagcatcta cattagttgg atttccaaga acatattctg ctacaattgg
     8461 atttatatct ggacagtttg cattttcaac tattggattg tatgggtctg ttatttgtgg
     8521 tctaactaat tttttagaat cttttaaagt tttcatagct atttttttag tatttttttc
     8581 gctagtataa tcgtagtttt tataattatg tttatagaaa tcttcataac tactatcttc
     8641 tttgttatag ctagaattta ttttgtcaca agattcatct ttatcatagt taaaattcat
     8701 ttcatcacaa gacttatctt taaacttatc ttgtttggag ttgtgaactt tgttagtatt
     8761 tttagtatgt tttttctcat gattgcaatc atctttaata tcagtcttgt gttcttgttt
     8821 acagtcaaga gctttgttca ggtagtcatg atgttcatgt tcacagttac attcattttc
     8881 aatgttttta gtatgttctt ttttccagct acaagtatta tcaattttct tgctcacttc
     8941 ttcatcatta taattatctg gtacaaaaca taagtaccag tccatacatg aatatttctt
     9001 attacaactg ttaactctat tttttaattc cttatagttt ttaggataag gtaaaatcac
     9061 tggcattcca ggaaaccagt ttgcaggagt aactatatta tctctatcac tagtctgaag
     9121 tgcatctact attctaagta tttctggaat atttcttcct gtagttagtg gataataaag
     9181 aatcgttctt agaatttgat tattatctat aataaataca gagcgaacag ttgatgtatc
     9241 actcattggt tttgatatca tgccgtataa cttggcaatt ctcatatctc tatcttctat
     9301 aataggaaat ggaatttcta cacctgtaag taaagaaata ttatacatcc aagctaaatg
     9361 tgaactgtta ctatcaacac ttagaccaat tagttctgta tttctttttt taaattcgtc
     9421 ataatattta gcaaaacata aaaattctgt agtacaaact ggtgtaaaat caccaggatg
     9481 tgaaaataaa acaatccaat tacccttata gtcagagagt ctaataggac catttgttgt
     9541 attggcttta aaatcaggag cctttgaccc taaacttggc aaatttggca tgtaaatcac
     9601 ccctcaataa attatcattt attttattct attaattaat acatattagt gttactattt
     9661 tattaaaagt taagtaatcc ttatgtcaaa aaaagatatt tattaaaaag atttaatatt
     9721 ttcctaatat aaatgacaat agtaattgct atttttttac taatttggta tagtaatgga
     9781 taaggggtag tgtcaatttt agtgattata cgaggtgagt ggattgaaaa aaattctaag
     9841 aattttagtt atctccatga tgatatgttt tgcaatgact tttgcattag tgttaataaa
     9901 ttcagttatg ggagtacata taggattgga ttattacttt ttgatgggaa gtgtgcttac
     9961 agtttgttta acaattggaa ttgttttatg caatgctaga aaaatatttg atagcaaagc
    10021 tatacacaag acaagaacga aaactgtcag taataataaa aaacaaaaga gtcattctag
    10081 ggatataaaa agaaagatat cataatttat gtaaaggaaa tatttatgta taaaataatg
    10141 attgataggt aaaataaaaa cacgatttaa cttgtgtttt tatttttttt caagaattat
    10201 gatataataa ccaataagaa atttagttta ccataaaata taaagattat taaatggaga
    10261 gggaactata atgaatttta gagatttatt tgagaaggct gtagatttta ttgatgaaaa
    10321 aaggaaatct atagttgcga tttcattatc aacattaggt gtaattgctt tgataatagt
    10381 atttttctta agtagtaacg agtttagtgt aggaaatgaa gctaatgagt tgttaaagat
    10441 tatagagaaa agacagtatt ctatagctgt tgattactac acaagtatag aaaaaaagtt
    10501 ttctgattca aaaatggaaa ggtttaataa gtctgtttct aaaaaaataa ataaattgct
    10561 attaaatagt ggtgataaat atttagatgg agacataagt caagaaagtt ttattggatt
    10621 aataaatacc gtgaaagaat taaacaaaat aagtatagat gtggatgact tattagctca
    10681 agctgataga gttagagaaa tgtataaaga agaaaatact acttatgata ttgccattaa
    10741 ctacataagt actgtttcaa tattaaatgg tatgggtagt aacctagatg tatacaaaca
    10801 gaatatagaa acaatcaaag agtctagaga tgtttatgac tcagcagtaa aagaccaaaa
    10861 agcatataag tattatgaag ctattgaaaa gtataataaa gtattaaaag aagatgaaaa
    10921 gtattacagt atggctcaaa atgggaaaga acaatgtatt gaagaaatgt atgattacta
    10981 tataagtaga gctgaagagg caaatacttc tggagattat gaaagagcat tacaatatat
    11041 tgagtattta aaagacgatt attcagatga tgaaaaggta cagagtttag aaaaaaaata
    11101 taaaaagaat ttatcattgt atactttaac atctgatgac attataaatg taatagctaa
    11161 aaaaagtggc aaagacaagg caaatctatc tataaattca cttccacaaa tgataaaaaa
    11221 taataaatac tattatgctg aagtatatga gtatgataaa ttaataaatg aagttttggt
    11281 aagtgcaaaa actaaagatt tatattcata taaagatggt aaaaaagatt acaaagtaga
    11341 ttatggtgat ggatacttta gaatattaga agatggaagt tatcaatttg gtattacaaa
    11401 agataaagct aagtttgtgt taactaatac tcttgatgaa aaagaaaata agtataaaaa
    11461 gattgagata ttagatatag agaaagcaga tagatatgta aaaagtaaaa agagtttaga
    11521 agaattgttt ggaaaatata aaaatattta ttattatgct gtagtaaata aaggcttatt
    11581 tagaggcaaa gaagtttatg ctattaatat ttacaatgaa aaaatatatg ccatatcaga
    11641 aaatggacta aatgaatatt aattaataat attaaattaa tatataatta aaaagttttt
    11701 ggggttttaa ggcgcattat gtagtaatat aataataggg atatataaat aattttattg
    11761 taaggagaag tatgggatga gtaatgtgaa caaaaagcta gttatagcta ttggagctgt
    11821 agcattagta gtaataattt ttataggcat aatgtgcatg caatttaaag gggagaaaat
    11881 agcaaaaaac acatatgtta atggagtaaa tattggaaaa ttaactaaat cacaagcaaa
    11941 acaggaatta gctaaaaaat acaaattaga gaatgttgat tttaattaca atgataaaag
    12001 ctggaaagta aagagcaaag atttaaattt atctactgac ctggataaaa cagtagaaaa
    12061 cgcatataat ttaaatagaa agagtgcttt ctttggcaat ctatcaaaaa ctatttcagc
    12121 aaattttggc aaaaaaagca acttagtagt tgcgataaat tatgataaaa ataaattaaa
    12181 agcagaaatg gaaaagatag ctaaagaaat agatgtagat gtaaaagatg ctacacttga
    12241 tataagtggt gaaaaagtca aggttatacc agattctgat ggtcttaaaa tggatatttc
    12301 taagagtatg gagaattttg ataatcaaac taaaaaaggt aactataaaa atgagttagt
    12361 agtaaaagct actccagcaa aagtaaaaaa agagcaactt gctaatatag atacaaattt
    12421 aggtacgtat tcaacaacat ttaaaacttc tcaaataaat agaagtataa acataaaatt
    12481 agcaacagat aatataagta atgtattact tatgccagga gaaactttct catttaataa
    12541 acatacaggg aaaagaagta aggaaaatgg atataaaagt gcacctgtaa taatggaagg
    12601 tgagatggaa gaagattatg gtggaggagt ttgtcaagtt tcatctacgt tatacaattc
    12661 tgtgctatac gcaggtttag aaattgttaa tgtaaaaaat catactatac catcaagtta
    12721 tgttccaaaa ggaagagatg caactgttgc agatagtgga atagatttct tatttaaaaa
    12781 taatctaaag catccagttt acataaaaaa ttacgtttca ggaaatcaaa ttgtttgcaa
    12841 tatatatgga agtgcagaag ataaacaaaa tataacaata tcaacaaaat tagatggcgt
    12901 ttcacaaact acaatgaaaa gagttaatga ccctacaatg cctaaaggta aagaaaaagt
    12961 agacaaaagt ggaagaaatg catattctgt atcaacatat agaacattta atgatgcaaa
    13021 tggtaaaaaa attaaaactg aaaaaatagc taattcatat tatcctaaga aggaaggtat
    13081 tatattagtt gggactatgg aaccaaaacc agaggaaaaa ccaaatacag acgaaaataa
    13141 gaataatcaa aatacaaaca atcaaaaccc aaataatcaa caaaaaccag aaacaccacc
    13201 aactgataat aagcctaatg agacacaacc acaaccgcaa gcttaaagtt ctcaagaata
    13261 ggatttatgt agagtaataa aaaatataaa tctaaatagg agggattact ttgaatttaa
    13321 ataatataac gaattacgtt caaagtattt gcgagaatat ttctaatgtc ttggatgtag
    13381 atgttacttt agttacaaaa gatttaacta ggatagcagg tacaggaata ttcagaggta
    13441 aaataggaga gacaatatca gagaagtcag tgtactctct tgttttaaaa gatgggaaat
    13501 cctatgttat aaataaagaa atagaagaga actgtaataa atgtgcaaat agagaaaatt
    13561 gtaaagagtt agctgatata tgtactcccg ttaaaatggg agagcatata cttggaatct
    13621 tgggtatagc tgcgtttaat gaagaacaaa aagaaaagat actttcaaaa gataaggagt
    13681 taactgcttt tgtaaggagt atgtctgatt taatatcttt taaactagat gaactttata
    13741 aacaagaagt taagactata gatgaccttg agagggaagc tatagaaaat gccataaaga
    13801 aatatggaag taatacagaa ggtatgaaaa aagtagctga ggcattaaat ataggtatag
    13861 ctacactcta tagaaaagta aaaaaattca acattaaaag ttaatagtat tgaaaaagag
    13921 gtaacctttt tatgaaaaga agaggattac cttttttatt atgagaatgg tggaaaaata
    13981 tgtatagttt tgacttgaca tattaaatat gttatcttaa atatatattt tagagtggag
    14041 gtgagtatta ttttagttaa agatataaaa caagacatag aaagcatgcc tagaaaaatt
    14101 ttagatatat ctttgataga agataataaa agtgatttga ctttttttaa tatagaagaa
    14161 gaagacggtg atgaaacatt aaaagcatta aaacagagtt atgtgactac aaattataaa
    14221 gaagaaactc tattaaactt gcaaggagct ttaaattcaa agtcagatac aacgtctaag
    14281 gagttaaaat tagaatcata taaaagatat aacaatgcat tggatttagc atataaaaat
    14341 tatataacta gtgccattaa tatggttaat aaagctctgg agataaatcc taaagacatt
    14401 gatatactaa atcttagagg tcttttaaaa ttattaaaat gtgattttgc taaatcattt
    14461 gagagttttt atactgcttt atgttatgaa aataatcata tatctagaaa atatgtaaat
    14521 ctaatttcat ctgaagattt taaaactttt ttaggaagat acaatcattc tataagattt
    14581 ataaatgaag aattaaatta tgagtcaata catattttag agaacataat tgaagaagaa
    14641 cctgaactaa tagagccata tgtaatttta tctttgttat atgataaact aggtaatgta
    14701 aaaaagagag aaggatattt agacaaactt aaagagctag ataaagataa tcctgtattt
    14761 gaaaaaaatg aagaagaaaa agaagataca agtaaaaagg aagaaataaa aaaagtaaaa
    14821 agaaagaaaa gtattttgcc atatattata gttggctgtg ttttaatagg tatggggata
    14881 tatttgattc aaagcaaaaa gagaatagaa aacttgaata accaaataag tagtaaagag
    14941 gaaaagttaa gtgaaactga taaaaagtta ggagaaacga gtaaaaaatt agaaaaaacg
    15001 aataaggaat taaatgaggt taaaaataaa gaaccagaaa gagagataac tgtagctgat
    15061 gaggaagatt tatattatca agctctaaat ttaaagaaaa acaaagaata tgaaaaagcc
    15121 atagataatt ttaaaagtgt agtttcttct ggtaaaacta aaaaatatat ttctgagtca
    15181 atatatcaat tagcaataac aaataaatta ttaggaaata aggatgaagc tataaaatat
    15241 tacaaaaaat atataaatac ttatactaaa aatgaccagt attatgatga ttcatattac
    15301 gagttgggta tgttttatta tgataatggc gatttaaaaa atgcacaaca gactttttat
    15361 agtttgagaa gtgaagttcc agacagtatg tataacaatt caaaaataaa agaaatttta
    15421 agtgaaaaat aggtttatat agttaagttt tttgaaacaa ccaagtatgt attttattaa
    15481 attaaaataa aaataaattt aaaaagagat tgtgtcaaat tataactttc tattttaaag
    15541 aaagatattt gcttgttgat aaaactacaa atatatgatt tgaagcaatc tctttaattt
    15601 ttggaggtgg aaaatgaaag ttttcaatca attggtaata attttatcta tctgggtaat
    15661 aggagagtat ataagttcct ttatacaagg aattatactt attcctggga gtatagttgg
    15721 tatgctatta ttatttctac tactacaatt taaagtttta gacttgagta gtatagaaaa
    15781 tgtaagtgga tttttcctcg ataatatggc tatatttttt attccagcag gtgtatcact
    15841 tataaagtca ttagacctaa taagggaaaa tgtattagtg cttttattag tcatatgttt
    15901 aagtacacta atagtaatgt atactactgg aattattgtg gagaaaatga taaaaaagaa
    15961 ggaagaagga caaaaaaatg tataatgtat tacagacacc agtatttggt ataatagtaa
    16021 caatagtttt tttcaattta ggaagataca ttcaacgaaa aacaagtaat cctatatgta
    16081 accctttatt aattgcaatc gtaggaataa tattgttttt aagcatatct aaaatccctt
    16141 atgagaatta caagatagga gctgatagtc taaatttctt tttaggtcct gtcacagtag
    16201 tacttgcagt tccactttat aaacaatttg aattatttaa aaagcatatg tttgagatta
    16261 ttgtaggaat aggttgtggt atagtgattt cttttgtatc tgtattgata attggcaaaa
    16321 tagctaattc agatgtaagt ataataaatt cactaattcc aaaatctata acaacaccaa
    16381 tgggaatatc tttagcaaat tctttaaatg gtgtagaatc aataacagtt gtagccataa
    16441 tttttacagg tatttttggt ggtatggttg cttctacagt attcaaactg gggaatataa
    16501 atcatccagt tgctaaagga attgctcttg gaacatctgc acatgcttta ggaactacaa
    16561 aggcttttga acttggagaa atagaaggag ctatgagtgg tgtttctatt ggagtctctg
    16621 gtacaattac agtaatacta attccaatta taatgaattt tatataaaaa ataaaattaa
    16681 gatgagtacc ttgaatactt aaaacaaaat aaaataaaaa aaatttaaaa taaaaactga
    16741 acgaaaaaaa tgcaaataat gaaataaaaa aatcatgtac tgaaattata aaaagaaaaa
    16801 tttcatttag gaattaatta taaagagaca attactaaac ttgaatttgc taaaataaaa
    16861 taaattactg ctaaaataga tatttagaaa aaaatactat aaatttacta tattgcaagt
    16921 taaataataa gcagtatcat attatgctag aaaaagtttc atttaaggca taaatgagat
    16981 gatttgaacg tttttaaaga ttagaatata aaagattgca ctagacatat atgaaaaaga
    17041 taatataata gatgatgtaa tagatgacag ttcaagaaat tataaaaaaa atttatttga
    17101 aatattatct attacatcag gaataatata tgtaagagta agcttttaaa attctaaaga
    17161 tttagaaaga ttgaagggag aaagaaatga gtgaagttaa gaatacccta caaaaacatc
    17221 cagttttaag atattcaaca atgctatttg ggatgttgat gacatctata ggaataaatg
    17281 ggttcttaag accagcacat ctgcttagtg gaggtgctac aggtatagca acatctataa
    17341 actatttgac aaatataaat gtaggtttat taacattttt aatcaatatt ccaatattta
    17401 tcttagggtt catatacctt gaaaaagagt tttgtatttc aagtttggta aatatgattg
    17461 tattttcatt attattagga gcaactcagg aaataagtaa tattatacct atacatgata
    17521 tattgcttca aagtgtatat ggtggtatat tatctggttt aggagtcgga gttgtattta
    17581 gaactaggtc ttctcaaggt ggtacagata taatagctgc aatactaaaa attaagaaga
    17641 atatagaaat gaaagacact gctttagcaa taaatggctt gatagtactt acaggaagtt
    17701 ttttatttgg gttggattta gcattataca ctcttatagg tctatttcta aatgcgtatt
    17761 caatgagttt tattaaagat gcaatgaatt accaaaaatc tgttatggta atgtcaaatg
    17821 aagtcgattt aatagcagaa gatataatga agagtttagt tagaggtgta acttttttag
    17881 atgcagaagg tgcatacact catcaaaaaa agaagattat atatacaata gtttcatcaa
    17941 atgagatacc aaagataaaa gatatagctt tgaagtatga taaaaaggcc tttatatctg
    18001 taaatgatgt tacagaagta aagggaagag ggtttaaagc aaaagattta tagaaaattt
    18061 tacaagctgg gagtttataa tgtaaatttg tatattatat ctctcagctt atttaaattt
    18121 agtatacgaa aataaaggtt ttattaatga tttgtgtatc actttaaaag atatcatttt
    18181 aaaattaaaa ttgaaaaaat tacttaatat gtagaaaaca tgaccactat taggatataa
    18241 taaatttaat agatttagta aaatacctag gaataacgtt ggaggaatgg cattatgaat
    18301 ttatctaaag aatatgttaa tttgtatgat aattatttga aatatataaa tgaagtaaac
    18361 aaagagatta gaagtataaa gaacatgaag aaacgtgttt taaaaagggg cgattttgct
    18421 ccggaaaatg aagtattaga attggaagga aaaacaatac gcgatataga tgatgtcgat
    18481 acaaaaaaac caaaaaataa aatctataaa ttttcaaatg gggatgtata tgtagggaaa
    18541 ttcttagagg gaaagatgga ggggaaaggc tcatatactt tttttgtaga tgaaggaaca
    18601 gttatggagt acataggaga atttaaagaa gataaaaaaa atggaaaagg aaactttata
    18661 ttttcaaatg gaaatgaata tataggttat tttgaggaag atatgatgag cggtattgga
    18721 aatatgttat atagtagtaa agatgaatat attggaactt ggaaaaacgg aaaaaaagat
    18781 ggaaaaggta tttataagtg gagtgatgga tgtatatatg caggtgaatt taaaggtgga
    18841 aagatggaag ggtacggtat ttgctataat agtaaagggg aagtacttta tgaaggagaa
    18901 tggaagaata atttgattca tggtaaggga acttatatat gggaaaaagg taaaaagtat
    18961 ataggtgaat ttatgcatgg taaaaaacat gggcaaggaa ctttttatct aaataatgaa
    19021 ttggtttatg aagggacttg gaaatttgat aagccatcta tatttgatag aacattagat
    19081 gaaatatttt ccttgagact gtagctttat ctaattagta gataaagctt ttttctaata
    19141 cttactgtaa ataggtatga atgatattga ttagtataca ctatcaatat taacttaata
    19201 aatgcaatat taaaaaattt tgattgattt taagaaggag agatatattg aagagattaa
    19261 ttgctttagt gttagtagta cttattgttt tattttatag tcctgaatta ttaaaagaag
    19321 gatatgattt aaaagaaaag ttttttcaaa gtgaaatatg gaatgaattt ttagacaaaa
    19381 taaatataaa tgataattct aagaaagata aaaaagaaaa aagtcaaaat ttacaatcaa
    19441 ataatactga aggggagaac ttaggagagt cagatataaa gaattttgaa aaaataagct
    19501 taggagataa tttaagctat gttttatcaa gtatcggcaa acctggtaga attgatatta
    19561 gtgaatatgg ttttgattgg tatgtatata atcaatatgg aaaagaattt gcaatggttg
    19621 gacttgaaaa tgatgaagta gtagcacttt atagtaattc tattaattca tgtgaaaatc
    19681 aagatataaa actaaatcaa gatagacaaa cagtgagaac taagattaca ccattaaaat
    19741 ataagagaaa aggaaatacc agatatataa taaactcaga aaatcaatat gatataataa
    19801 gtaaggaagg taagtatatt acaattttct acgatattca tgaagaaaat agagtttgtt
    19861 catatttaat aattgataaa agcactgaag atgagtttaa aaatttatat ccagatgata
    19921 gcgaagaact taaaaaatgt tttgaacttg aagttataga ccttgtaaat agtgttagaa
    19981 atcaaagagg gttaaactca ttgagataca gtgaacaggc aactttgagt tcaagaaaac
    20041 atagtgaaga tatgagagat aataattttt ttgaccatgt aaataagaag aatgaaactc
    20101 catttgacag aatgaaaaga gaaggtatag tatatacttc agctggagaa aacatagcag
    20161 caggacagat taatgcaata tatgcgcatg aagcttggat gaactctgaa ggacatagaa
    20221 aaaatatttt ggggaactat aataatattg gagttggagt aatatttgga ggaagctata
    20281 aaacatatta cactcaaaat ttttataaat aactaaaata tttgaaagga agttaaaaaa
    20341 tgacatcatg gaaaaaaaag acagtatata agtgcttaat agcagtagcc ttgttttgtg
    20401 gaatagtgtt aatatctaat tttagtaaag tttctgcttt gatgatggat actaatggaa
    20461 atgttttgat taaacatggt tcaagggaaa aaaagctaat agcaattacc tttgatgatg
    20521 gtcctcatcc taaagaaact agtcaagttt tagatgtatt aaagaaatac aatgtaaaag
    20581 ctactttttt tatagctgga aaacatgcaa aatggtataa agaaccatta gtaagagcaa
    20641 gtaaagaagg tcacgagata ggaaatcata ctttcaatca tcctgatata agtaatttaa
    20701 gttctagtca gatagaagag gagattgtta agtgtgaaga cattttgaag gaagtaactg
    20761 ggaaaaaacc tacattattt agaccaccat tcggaagtta tagagaaaaa gacttaatag
    20821 aaattgcaaa aaagcatgac tataaagttg ttttatggac aggtgtagat gtgaaagatt
    20881 ggaaaaatcc aggagctaat agcattgcag ataaaataat taataaagta caaaatggag
    20941 atataatact tttgcatgat tatgcaacta atgatacagt tgaagcttta gatatgttta
    21001 ttccaaaaat gattgaaaaa ggttttaaat ttgtaacagt ttctgagttg ataaaataat
    21061 ttttaggggg gagatttaca gtatgattct tatgatagac aattatgatt cttttgtata
    21121 caacctagtc caatatatag aagaattagg ggaagcagtt gtagtaaaaa gaaataatga
    21181 aatcaaaata agtgacattg aagaactaaa tccagaggta attgtgttat ctccaggacc
    21241 gtgttctcca aaagaagctg gtatttgcat agatattgtt gagcatttta aggggaaaaa
    21301 acctatacta ggaatttgtt taggtcacca aactattgga catgtttttg gaggagatat
    21361 tataaaagca caacagcctg tgcatggaaa agtatatagt atcaaccata ctaataaagg
    21421 agtttttagg ggacttaaaa atcctttaaa tgtcactagg tatcattcac taatagttga
    21481 ctccaataca gttccaaaag aattagaaat tacagcaatt actgataaag gggaaataat
    21541 gggaattagg cataagaaat atttaataga aggagtacag tttcatccag aagctatttt
    21601 gtcagagtat ggtcatgaaa tgcttaaaaa ttttataaca gaagctagag aaagggtaca
    21661 tatgtaatat gataagagag ataaatacta aattaaattc ttttgagata tttactattt
    21721 ttagaaatga acatgacagt tttatattag atagtgctat ggataaagaa aaattgggaa
    21781 gatattcttt tataagtagc caaccattta aggtattaaa gtataaagat actgatgaaa
    21841 atcctttaga agttttaaaa gaagaactac ataagtatag ggttgtaaat gatactaatc
    21901 ttccatttgt aggaggtgct gtgggatatt tatcttatga tttaggtaat tacatagaaa
    21961 atttacctag aactgctgta gatgatattg agatgcctga tatgtatttt ggattttata
    22021 atcatgtaat agttatagac catcttgtgc aaaaaactta tatagctact ccaaacatag
    22081 atatcgaatt agaagaaaag ataatagatg atatagaaca aagaatatta aaagaagaga
    22141 aaaaaggtat agatagcata tgttatgaag aaaaagaagt aacacctata agacttaaat
    22201 ctaattttac aaaggaagaa tttaaaaatg cagttcaaag tgttagagag tacataagac
    22261 aaggagatat atatcaagct aatttaacac aaaggttcag tggagaaaca gaacttacaa
    22321 gctttgaatt gtatagggac ttaagaagat ttagcccagc accatttggg gcattcttaa
    22381 actttgaaga tgcacatata ttatcaaatt ctccagaaag atttataaga tgtgttaata
    22441 agagaataga aactagacca ataaaaggta ctcgtccaag agggaaagat aaagaagaag
    22501 atttaagact tcaacaagaa cttagaaata gtgaaaaaga tagagctgaa ctacttatga
    22561 tagtagattt agaaagaaat gatattggga gaatatctaa aactggaagt gttaaagttc
    22621 cagagctatt tgtaattgaa ccatatgcaa atgtaaatca cttagtttcc acagttgtag
    22681 gtgagttaaa agatgataaa gatgctactg atgtaataaa agcaactttt ccaggggggt
    22741 ctataacagg agcacctaag attagagcaa tggaaattat agacgaatta gagccaacgc
    22801 aaagaaatgt atatactggt tcaataggtt atataggttt taatggtgat atggatttta
    22861 atatagcaat tagaacaata atcaaaaatg acaaaaaagt ctactttcaa gttggaggag
    22921 gaatgacttg ggattctgac ccagatgaag aatatcaaga aacacttgat aaagctaaat
    22981 ccataatgaa ggctttgagg ggatattatg aagaataatg taggttttga tagtagttta
    23041 agtaaatttg gaattggttt gtttgaaact attagagtta aaaatggaat agcagtagat
    23101 ttagatatcc atatagaaag aatgctaagc tcaataaatt gtttggattt agatataaac
    23161 tataaaaaag atttcttgat aaatgaaatt gttacatata taaaaaaaga aaatgtcatt
    23221 aataaagcac ttagaattac tgtttttgat gaaggttata atatatctat tagggatatt
    23281 ccatataatc aagagacgta tgagaagggg tttaaattag ctatatctcc aatagttagg
    23341 gggaatagtt taattcatag acataagact actaattatt ttgaaaacat atataccaaa
    23401 aatatcgcaa ataagactgg ttataatgat ggtatatttt taaattcaga aggtgtaata
    23461 ttagaatgtt ctatgagcaa tattttcttt ataaaaggag aaagagtgta cactcctagt
    23521 gatagattac cgatattaaa tggaataata aaaaaaagga ttatagagat ttgtgacgaa
    23581 ttacatatag agttgataga aaatgaaatt aatatatcag aaattagtag ttttgatttt
    23641 gtttttgtca caaatagtct tatgggcgct ataaaggtta cagaaattga caaaataaaa
    23701 tttaataaag aaaatgttgt ttttgataaa atagtgaagt tattagaata aattaaaata
    23761 ttaacaattt gttggagaca aaagatgata ggttgtattt atgaagtata caatgaagag
    23821 atgactaaaa taatagaaaa ccaaaacact ttggctgtat atttagtggg ttctagtaaa
    23881 gatgtagatt ttactcttta tgatgtagtg ataaacgata ttgatgtgtt cgtttttgta
    23941 aaagaagggg aaaaacaaga gagaatactg tttaaaaaaa agggaataga atttgatgta
    24001 aattactttt caaaagatgg tgtaaaaaaa ttattatcaa atagggaata tttctttgtg
    24061 aaagaaatga aagatgctaa agtactgttt gatagggaga atatatcaca tattataaaa
    24121 gatataagta gagatattta cttagaagga cctagtaaaa tgtcattaga ggaaaagtct
    24181 tttataaagc aagatattgg agcaaaaata ttgaatttaa aaaataaaga aaaatttgat
    24241 gtttttgaat atcatttttt gacgaattta tatttaaaag atataataat tgggtacttt
    24301 aatataaatg ataaatgggt accaaaagac aaaaaattgc taaaagtttt aaaaaaagaa
    24361 aataatgagc tttttgaact agtgtcaaaa gtaagtgaaa attatgacta tcaaagactt
    24421 ttagatgttt ataattatat ttttaaagaa attgagacaa aagaggttat aaaactaatc
    24481 ttctagtcac aaatcaatgg ggggagttta aatgaataaa gttgataaag aaaagataca
    24541 acatgcagta agagaaatac tagaggcaat tggggaagac ccagatagag aaggtctaat
    24601 cgaaactcca aatagagttg ctaggatgta tgaagagata ttctctggtc ttagtgaaga
    24661 accaagagac catttaaaag ttctttttgc agatgaaaaa catgaagagt tagtattggt
    24721 aaaagatatt ccattttatt catgttgtga gcatcatcta gttcctttct ttggaaaagc
    24781 tcatattgct tatttaccaa aaggaggaag acttacaggg ctttctaaac ttgctagagt
    24841 aatagataca ttggcaaaaa gaccacaatt acaagaaaga atcactaaaa atgctgcaga
    24901 tataataatg gaagaattac aaccttatgg tgtcttagta gtagtagaag ctgaacatat
    24961 gtgcatgaca atgagaggcg taaaaaaacc aggttcaaaa actgttacat cagcagtgag
    25021 aggaatattt gaaaaggaca ttgcgtctag agccgaggct atgagtttga ttactatgaa
    25081 gtagtaattt attatagata taatcattta ttgagataaa atttaattta agggagtgtc
    25141 attaattgtg tttgattatg gtaaaagaac ttatataatg ggaatattaa atgtaactcc
    25201 agatagtttt tctgatggag gagattttaa taacttggac atagcaatac aacatgcaaa
    25261 agatatggtt gaccaaggtg cagatataat agacctagga ggagagtcta cacgcccagg
    25321 tcatagctat gtagactcag acgaagagct aagaagagta atccctgtaa taaaaaaact
    25381 taaacaggaa ttggatatac caatatcaat agacacttat aaggcagatg ttgcagaaga
    25441 agcattaaaa ttaggtgtca ctatggtaaa tgatgtttgg ggtcttagaa aagataaaaa
    25501 tatggcttct gtaataggaa aatatgatgc agaagtgtgt ataatgcata atcaagatgg
    25561 gacaaactat gacaaagata taatggagtc aataaaagac ttttttaagg taagtataga
    25621 aatggcaatg agttgtggtg taaaaaaaga aaaaatagta cttgacccag gtgtaggatt
    25681 tggcaaggat tttgaacaaa atattgaagt attgagaaga ttaaatgaat taaaagattt
    25741 agggtatcct attttgcttg ggacatccag aaaatcagta ataggaaaag tacttccagt
    25801 tgagcctaaa aaaagattag aaggtacaat agctactact gttcttggta taagagatgg
    25861 cgtagatata gttagagtac atgatgttta tgaaaattta atggcagcta gaatgactga
    25921 tgcaatatat agaaagtagg tataatataa tggataaaat attacttagt aatctaggat
    25981 tctatggata tcatggtgtt cttaaagaag aaaattttct aggtcagaaa ttctttgtcg
    26041 acatggaact ttatattgat tcaagagaag caggtcttag tgacgatata aataaatctg
    26101 taagctatgc cgaggtttac aatgttgtta aagatattac tgaaaataaa caatttaatc
    26161 ttttggaggc tttagcggaa aatattgctg aagaagtctt gaataaattt atacttataa
    26221 atggagttat ggtcagagtt agaaagcctg aggctcctgt aaatggtatt tatgactatt
    26281 ttggtgttga gataaggaga acaagagatg aataaggctt atttgggaat aggtactaac
    26341 atgggtgata gatttgataa tttatcaaga gcatgtgaac ttttgaaaaa tagtgattct
    26401 atatataaag taaaagaatc aagcttatac gaaacaaaac catggggata tacagaacaa
    26461 gcagattttc taaatatgtg tgtagaaata gaaacagagt ttgaacctta tgagttgtta
    26521 gaatattgtc aagaaataga gagagaacta cacagagaga gaatagttca ttggggacca
    26581 agaactatag atgtagacgt actatttttt aatgatgtag tttccactga tgaaagacta
    26641 acaatacctc atccaaggat tcaagataga gctttcgttt tgataccact tatggattta
    26701 aatgaagaac ttataataaa tgaaaaaaca ataaaagaac atttaaacct cctatctgct
    26761 gaagaaagag aagaagtaaa ggagcttgta ggttatgaaa gaaaacctat ttaataataa
    26821 ttcaaagata gagataaata caaataacaa aaagataact gtaaaaaaag ataaacttat
    26881 aatagctgga ccatgtgcaa tagagagtta tgagcaactt ttggagactg ctaaatttgt
    26941 aaaaagtcag ggagcaaata ttttaagagg tggagcatac aagccaagaa catctccaaa
    27001 ttcatttcaa ggattaaaaa aagaaggtct tgaaatatta aaagcggtta aagacgaagt
    27061 tggtatggct gttattacag aacttatgga tgtaagagat atggacgaac tgtatagtat
    27121 aagtgatata attcaaattg ggtcaagaaa tatgcaaaac tttactcttt taagtgaagt
    27181 gggaaaacaa aataaacctg ttatgttaaa aagaggaata gcttccacaa ttactgaatg
    27241 gataggtgct tctgaatata tagcaataga aggaaatagt aatattataa tgtgtgagag
    27301 aggtataaga acgtataatg attatactag aaatacatta gatttagctg cagtacccat
    27361 tattcaaaag gaaacaggtc ttcctgttgt ggtagaccca agccatgcca ctggagttag
    27421 atatttggta aaacctatgt cactggcatc ttttgcatgt ggagcagatg gaataatggt
    27481 agaagtacat cctgacccag aaaatgcttt gtcagatggt gctcaatctc tttgctttaa
    27541 tgaatttgaa gatttaatga aatcaattaa taattattaa aagaggcaag agcctctttt
    27601 ttattcgtaa tcgttgctaa ggtaaattta taagttataa tttgatagtt agattattta
    27661 atataaataa agatttaata ttgatagaat taatataaaa aataaaaatt ttttgattaa
    27721 taagaaggat atcaataaaa aaagtcgaat tgtgtatatt gagtgattga caaatttaga
    27781 atatagtgta gaatagaaat gttaacacaa aaaattacac cagaaggtga taatatgaat
    27841 gatataaaag acattattga agaaataaaa gctagatgcg atatagcaag tataatatct
    27901 gaatatatga atataaaaca atctggagct aattacaaag gattatgtcc atttcatggc
    27961 gaaaagacac cttcttttta tataaatacg tcaaaacaga tttataagtg ttttggttgt
    28021 ggtgaaggtg gagacgtaat aaattttgtc atgaaaatgg aaaatttgga ttttatggat
    28081 gctgtaaaaa ttttagcaaa taaatgcggt atagaaatta acactaacat gaatgaagaa
    28141 actaggatta aaatagaaaa atctaagaaa tttcaagata ttcatacgga agcagctaga
    28201 ttttatcttt caaacttatt gggaagcaaa aatcttgggt atgaatattt aagaataaga
    28261 ggactagatg ataaaattat taagaaattt ggtttaggat tttctttgga ttcatggaat
    28321 tcacttatga atactctaat cagtaaagga tataaaaaac aagacttatt ggagtgcggt
    28381 ctaattgcaa aaaatagaga tggaactaat tgctatgata aatttagaaa tagagttatg
    28441 tttcctatat tcgattatag aggaaatata ataggatttg ggggaagagt attagacgat
    28501 tctttgccaa aatacttaaa ttctccagac acgttaattt ttaataaaaa acagaactta
    28561 tacggattaa attttgctag aaaaaattta gaaagtaaaa ctatagtctt agttgaagga
    28621 tatatggatt taatttcgct ttatcagtat ggtataaaaa atgtagtagc aacccttgga
    28681 acagccttga ctgaacaaca aggcatttta ataaagagat atgcggacac tgctattata
    28741 tcttatgatt ctgatgaagc aggaataaaa gcgactttaa gagctataga tatacttact
    28801 aaattaggta tcaatgtaaa agtgttagac ttaaaagatg ctaaagaccc agatgaattt
    28861 gtaagaaaat acggacttag tgattataaa aaagctatgg atgtttctac tcactacata
    28921 aaatataaaa tagatcatct taaaaaagaa tttaacattc aaaaagatga agaacgagtg
    28981 aagttcgcaa aagaagcttc taaaattata aaacaattga caagtcctgt tgaaattgat
    29041 ttttatacaa agtatttaag caatcaaata gatattaatg tcgagtctat aaagagagaa
    29101 gtttatggta aaaattacaa taagccatat aataacaaaa atcaaaagaa aatagaagaa
    29161 aaagtaattg aaaaagtcga agtcagacaa gacggaaaac aattagttga agaaactcta
    29221 ataaaaataa tgttagaaga caaaaaaatt agagaaatag cgttacttaa agtagaagaa
    29281 agtgattttt tattgaaaga aagtaaagaa attttaaatt acatgattaa aaatcaagaa
    29341 ttggacaaaa taactattga caaattgaaa agtttaaaca tatccgaaga atacttaaaa
    29401 gaattaaatt caatttcttt gaatagtata aacttagaga acacaaaaga aatagaagga
    29461 ataataacaa atatcagaaa aaactcatta gaagagcaaa ttaatagctt gttgagagaa
    29521 caacaagaat tagaaaataa taatgatatg aaagaggtag atggtagagt tatggaaatt
    29581 gctttaaaaa tagttgagat aaataaaatt ttaaagagct tgtagagaaa ggaggtttga
    29641 gtgtggaaaa taaatcgaat aaaaaagagt taaaaaaggt tactgctaag acattaatag
    29701 aaaaaggaaa aaagcaaggt tctctgacac ttgcagagat aatggaagct ttttcggaga
    29761 ctgaacttga taaggatcaa gtagaaaatc tttatgagac tttaggaaat ttgggaatag
    29821 aaataacaga aacaaaaaac tataaagctg atatagattt ttcggttgct gatgacgatt
    29881 taagtatagg ccacttagat gaagatgcag aggcaatttc acatgatgac tcttctgcaa
    29941 tagaaataga aactgtggat ttatctttac caaaagggat aagtatagat gaccctgtaa
    30001 gaatgtactt aaaagaaata ggaaaaattc ctctacttaa accacatgaa gaagtagaat
    30061 ttgctagaag gatgcacgaa ggtgatgaga tagcaaaaca aaggttagtt gaagcaaact
    30121 taagactagt tgtaagtata gcaaaaagat atgtaggaag aggtatgctt ttcttggatt
    30181 taatacaaga gggaaattta ggtcttataa aagcagtcga aaaatttgac tatacaaaag
    30241 gatataagtt tagtacttat gcaacatggt ggataagaca agctataaca cgtgctattg
    30301 cagaccaagc tagaactata agaataccag ttcatatggt agagactata aataagctaa
    30361 taagagtatc aagacaacta cttcaagaac ttggaagaga tccaaaacca gaagaaattg
    30421 caaaagaaat ggaaatgaca gaagataaag taagagaaat aatgaaaata gctcaagacc
    30481 ctgtgtcttt agaaacacca ataggagaag aagaagatag ccatttaggt gactttattc
    30541 cagatgatga tgctccagcc ccagcagagg cagcagcata ttctttatta aaagaacaaa
    30601 tagaagatgt acttggttca ttaaatgata gagaacaaaa agtattaaag cttagatttg
    30661 gtcttgagga tggtagagcc agaactcttg aggaagttgg aaaagagttt gatgtaacta
    30721 gagagagaat aagacagata gaagcaaaag cactaagaaa actaagacat ccaagtagat
    30781 caaaaaaact tagagattat ttagactaaa aagatacatc attgatgttt ttgtgataaa
    30841 tgccatgaga taagtgatat ctcatggcta ttttgtagta aagatttaat tttatttttg
    30901 tagttatttc agtctaatat ctataacttg gattaaatcc tataaataaa gggaattgaa
    30961 aggagaatta tatttgaaat taacagatag attattgaaa atagcatcat tagtgtcaga
    31021 tggtaaaaaa atagctgata tagggacgga ccacggatac ataccagtat atttgttaaa
    31081 agagggaaaa gtaccatttg cagtgctagc ggatgttaat aaaggacctc ttgacaatgc
    31141 acataaagaa gtcatacaga acaatctttt agataaagtt gatttacgat tgggttctgg
    31201 tatagagata cttgaaatag gggaagtaga agaagtgata attgctggta tgggtggaat
    31261 cttgataagt gagcttctcg aggctaaaaa agaagtagca cataatgtag aaaaattaat
    31321 attacagcca atgcaagcac aagaagaact tagatattac cttttaaaca acggatatga
    31381 aattttagaa gaagttttag tgagagaaga ttttagaata tatgagataa ttgttgccaa
    31441 atatacaggt aaaaatacta taatagaaga tgagatatat tatgaggttg gaataaagct
    31501 tttagaaaac aaagattctc tttttaatga ttttatagaa aaaaagataa agacatatag
    31561 ttctatagtt aataaattag aagggaaaaa tggggaagct atagataaga agagaaagga
    31621 aagcgaagtt gcaataaaaa aacttgaaaa tttaataaaa taaataggag gtatatatgc
    31681 tattaaagag tctaactaga aaaattgaaa aaaagtatcc tctaaactta gcagaggatt
    31741 gggataatgt aggtcttata gttggagatt ttgatatgga tgtaaaaaag gtattggtat
    31801 ctttagaagc taatgaagat gttattgatg aagctatatc caaaaatata gatttaattg
    31861 tgacacatca tccttttata tttgggaaaa ttaataaaat taatagtgga gatttaaaag
    31921 gcagacttat ccaaaaactt attaaaaatg atatatcact ttattctatg catacgaatt
    31981 ttgatatagc atttgatggt ctaaatgact attttatgga aattatggaa tttggtaatt
    32041 caaaggtttt agatataact aaaagcgaaa atttgtataa attagcagta tacgttccac
    32101 ataactattc agatgaactt agaaaggttt taagtaattc aggagcaggt catataggga
    32161 attatagtga ttgtacgttt tcaatagaag gtgaaggaca atttaagcca ttagaaggct
    32221 ctaatccgtt tcttggaagt gtaaatgata tagaaactgt aaatgaagtt aaaattgaaa
    32281 ctgtagtacc acagaagctt ttaggtgggg taataagttc tatgttagat gcacatcctt
    32341 atgaagaagt tgcatatgat ttgtacaagt tagaaaataa aggtgagata tttggactag
    32401 gtagaataag taaacttgat aaaagtatga cattagaaag tctttcaagg aaaataaaag
    32461 agaaattaaa tatgaaacac ataagagtag ttggaaacct tagtacagac ataacgaaag
    32521 tagccgttgt aacaggagct ggttcagaat ttgtaaaaaa agctaagaga caaggtgcag
    32581 aagtactaat tacgggtgat gtaaagtatc atgaggcaca agatgcactt gatattggaa
    32641 tgtgtatagt ggattgtggt cattttgata cagaagatat attcaaaaat gttatgaaga
    32701 gatttttaga tgaatttagt gaaattgaag taataaaaag taatgtatat ttgaatccat
    32761 ttagtactat ttagcagtgt ttgataataa aaatactata taaaaaataa atttgagttt
    32821 aattaggggg ataatgatga aagtagcagt aatttttcat agtgtttgtg ggagtacata
    32881 tttattagct agagaatata aagaagcgct tgaagaaatg aatatcgagg tagatatatt
    32941 tagagtaagt gacgaagttg caaaaacact tcctcaatat tatttgataa attccaaaga
    33001 atacaaagac gaatttgaaa gtataaatgt aattaaatct ggaaaagaaa tattagatta
    33061 tgatgctata tttatgggtt caccaacata ttatggaaat gtatctggac caatgaagat
    33121 gtttatggat agtttttctg atatttgggt aggtactcct ttgagtggta aaatatttgg
    33181 ttgttttgct acagcaggtt cacaacatgg aggaggagaa ttagctttgc aagctatgaa
    33241 tatatttgcc caacatatgg gaatgactct tttgtcagtt ccatgttcag taagaggagg
    33301 ttatccagcg tatggaatac ttcatatagc aggagataat tctgatataa gaccaaatga
    33361 tgatgccaag ataggtataa gagattacct aaaaagactt aacatataag aatatcatat
    33421 aaaaatacct aaaagcgact gtatatactt ttaggtattt ttacttaatt ataaacgtga
    33481 agctttacca gaaatatggt caatttctat tttaacaaca gttgttaagt tcatatcctt
    33541 ctctatgtac ttcatgcctt cttcaaagaa tcctttagaa tattttttta ttatttctac
    33601 aagagcattt ttcttttctt cattttctac agtgcaagct tttccaaaaa ctatagcact
    33661 agaataagta gtactaaatt tatctggaat aacactttca ttagcaacta ctaaaaaaga
    33721 aactttgttg tttttcgata tattatctaa cttatgccca tttcttgcac aatgaaagta
    33781 aatagaatca ttaaagtata cataatttac agcaactcca taaggataac cattttcaga
    33841 tatagtggaa aaagtaccaa attcaccatt ttttaatact tcaacagtat cttcttttgt
    33901 catttctctt tttttcaatc tcatttcttt aaacataata tcatcctttc taattttgat
    33961 gcttaaatta aattttagaa catttataaa gattatgcta tgttttttac aaattttaag
    34021 aaaaaaatat agtttaagat aaaaggtagt gaaagagaag ctatttggag gtttatttat
    34081 gatattattg ttagagtagt ataaacaata ctaataaatt taatagctgg tttcttgtta
    34141 tttaagatag taagcatgtc tataagaagg ggaagttatc gagggtagaa gttgttattt
    34201 ataactatat tataaagaaa atattattat ataaaataat aaatagtaaa gaggtatcaa
    34261 gttgaatata atacttaata cctcttcaaa attaagttat aaaagtgttg tgggaattgt
    34321 cctatacatg gtaaggagat aataaagaat gtatctttaa agtctcaact aaacagcaaa
    34381 atttatccaa caaacataca ataaatgctt ctttgtatct aggtggctta acagtaagtg
    34441 gccacatgtg ttttaaaatt atgtccgttt ctttttcatt tatttgaaaa aataaagttg
    34501 cattttttaa tgcttctcta gggtgagtaa atccatgtaa tccttttcta tcacctttgt
    34561 aatgccaatc ataaagaaaa aagtcgtgta gtaaagctcc tctaataaca ctttgaatat
    34621 caacacttaa gtgcaatttt ttacataata gataactata atatgctact gacaaactat
    34681 gttcaagaca tgaaacattg ccatgatgag gaaatgttct cataagttga actttttcag
    34741 aagaaagtat ctcagaagca cattcattaa aatatttgat ttcagctttt gacaacatta
    34801 tgaaaatctc cattcttagt aatatataaa tactatactt caacgataat atgaaatatt
    34861 tcttatccta caaaatataa tacccaataa tacaagtaag atacctttta tggtatttta
    34921 aagtaagaga gattttatta ggctaaacaa tgaaaaaatt aaaagtaaag ctatagcaat
    34981 atatagtatt tttccaagag gattgccaaa atttatagtc catccaatac caaacctttt
    35041 attaaccatt aatgatgggt catttggatt attatacatg atgcctgcta tccaatactt
    35101 ctcatcttct tctggagaat aagatgagtt gaattttaaa tttggggaat taatatatgt
    35161 tgctattaaa taaattataa ctaaaagcgt tgtaatattc atagcagttg atagttttgt
    35221 ttttattgat aaaagagtag taaaaaactg tgttgtcatt ataatcatca atattaaaaa
    35281 caaataacca attttattta aatatttaat gttttcaact ctactttctt caattctatt
    35341 tgtatctatc ttaatccttg actttatgga tccaatggaa gttatatata ataaaaccat
    35401 taaacaaaag tcagtaccga ttaatttaaa tacattaaga aatgatttat ctataaaaac
    35461 atcaggagac ccatttatgt tccaatgagt ggcaattgag tctggcaatt gattataatt
    35521 aaatgctata aaaatactca ttcccaaagt aaatagtatc ggtattaaat agattatttt
    35581 aaattttttt attattttat ttttcttatt tataaaatcc atatctacta ttaattttga
    35641 atctatatct atatgaccta atttagaata aatttctgat ttaagcttct tagctttttt
    35701 atgtgtatga atatatagta tactttcata taataaaaaa cctataatac caataaaata
    35761 ggaaagttcc aatttattaa atgtaaagac cagaataaaa actaatataa agtctataat
    35821 aaaacctaaa gataatagtt ttttaaattc tttatctaac tttataaagt aattataatt
    35881 tttatgctct tggtctatag aaactccata gatgtgtttt ttacctacaa aatagggcat
    35941 aaaataaaat gtagcataca tcaaaattag aactggaaat attgtaaata gacattcaat
    36001 cttactcata atatttcacc tcatacttgt caaataaact tgaacttagt gacaaaaatt
    36061 cttctttact aatacctgat aaataagatt ttgccaccaa taattccaag tcaagtttat
    36121 ttttttgacg agttgtatta tctattttca ttggtagttg agctacaata gcaccttttc
    36181 ttctatcaat atttaagaat ccctcttctt ttaattcatt gtaggatttg ttcactgtat
    36241 gtaaatttac accaatattt tcagccatac ttcttacaga tggaagtgat tcatttattt
    36301 tcaattctcc tgaagctata gccttagtta tttcttcttt aatttgtaca taaatagatt
    36361 tatcactatt aaaatctaaa tttaaaacca taaaaaacct ccattatgct ttataaatga
    36421 aaacgattgt tatatacata gtataacata aaaaataata acaaacacaa aatataagtt
    36481 tatattttgt aaaaattgta atttaaaaaa taacataatt tttattaaaa taatacagat
    36541 actatttata gatagaaata aaaattagaa aggagaaaaa atatgttaac atatttaatg
    36601 aaaagacaaa tgcataaaaa agatgatgga atgagtaaac caatgatgat tacaggagct
    36661 gtaattacag gtgtaggagc atatgctgta tataaaatgg taaaaagcca taaatctaat
    36721 tctcaaatgg tcaatacaaa ttattatgga aatagcgatt atgatgatta taaatatgac
    36781 gaatttgatt atgactgtga ttgtagagac acaaaacaag attatgaata tgaagaatta
    36841 aaaaaaaaag taaaagaatt taattcaaga agaataaatt gtgaaaattg tccacatgtt
    36901 tctcaagaag aaatgatgca atatgtaaat agtgttaaag atgtaaagga agagattgat
    36961 ttacatgaag atgctaaaaa agataatatt tacaaagaag atgaatacaa aaaatatgaa
    37021 gaagaataag taatattttg tataactgaa caaatagtaa tgtcaaatta tttaatatct
    37081 agttttaata tgtaagttgt aacttaactt gaaagaatca gtatcattct ttagatagtt
    37141 aagtaacaac ttacatttta tttttataaa ttttaatagt tctttgtgac tattttgtaa
    37201 ttttagaaat attttagtat ccagggtaat tgtgtataat aaaacttgta tagatagata
    37261 gtatgaaatt aagcagatag tttaaaatta aaatgaaaag aaaggaaata gttatgttaa
    37321 agttgttgat agttgaagat gatagtacta tatcttttgg aataaagtat gctttagaac
    37381 aagaaggatt tagtatagat atatcaaagg atttatctag tggaaaagaa atgatttctt
    37441 ctaatgaata tagcatgata ctgttggatg ttacattgcc agatggaact ggttatgagc
    37501 tttgtcaata tataagagga ttctctcagg ttcctataat attcttgact gcttgtgatg
    37561 aagaagtaaa tattgtaatg ggtctcgata taggtggaga tgattatata acaaaacctt
    37621 ttagaattag agaattaata tccagaataa aagctgtttt aagaagaaaa ggtaatactt
    37681 cagaagaaaa taagaaaata cttaaatttg gagatttaag tatatataca ttagaagcta
    37741 gagtatataa aaatgataaa gaaatatttc tgacatcagt tgaatataaa cttcttttaa
    37801 ttcttataca gaataaaaat actgttttaa gtagaactca aatattggaa aaactttggg
    37861 atgtaactta tgattttgta aacgacaata ctctaactgt ttatataaaa aggcttagag
    37921 aaaaaatagg tgatgattta tgtgaaccaa tctacataga aactgtaagg gggattggtt
    37981 ataaatggat aggaagtgaa aatagtgttt ctatataatc cagaagtgaa gaagttttta
    38041 agtaagtatg ttactttgat gtttattgtt atcataatat ctattggatt tagcataatc
    38101 aacgtgtcct taactaaaga gatggttgta agaaataatc aggctataat aggaacttta
    38161 tcaagcaagt atccaaactt ggaaagtcag atagtagaca taataactca agggaagtct
    38221 atggagaaca tagattatgg tcagaaaata ttaattaagt ataattatga taaatctatt
    38281 aggataaact ctgagcctat tatttctaaa ttagttttag agactatcaa aataaatata
    38341 atattagtat gtataatatt tattttgata tttgtgttgg ttgttagata ttttaagtca
    38401 atttacaatg atttatcaga tatgactaag tatgtttact atagttcaga gggtaagcca
    38461 tttgatatga aaaacaagaa tcaagaaggg cagataggtc ttctaaaaac tgaactctta
    38521 aagatgacaa caattcttaa tgaaaaggtt gaacttttaa aaagagaaaa gatattttta
    38581 aacaatacaa tttccgatat atcacatcaa ttaaaaactc ctatgacttc tttaataatg
    38641 ttgaatgatt tattatataa tgatgtacca tacgaagtaa aaatcgattt tttaaataag
    38701 ataaaaaatt aattaaatag aatggattgg ttgattaaaa gcatgcttaa attatcaaag
    38761 gtagaagcaa aagtgataaa ttttaaaaaa gataaggtaa aatttagtga actcatacat
    38821 agagcaatgc tatctatgaa aatacctatg gagattaaaa atcaaaaact gactatagag
    38881 ggaaatgata atgtatctta tattggtgat attgattggt ccgtggaagc attagtgaat
    38941 ataatcaaaa attgtgtaga acatactcct gaatttggaa atataattat aacctataaa
    39001 gaaaatccat tattttctga actaataata aaagatgagg gtgaaggtat agataaaaaa
    39061 gatataccac atgtatttaa acgattctat agaggaagaa gtagttcaaa agaagatagt
    39121 gtaggaatag gtcttgcaat gtcaaaatct ataatagaaa gccaaaatgg cgatatatat
    39181 gtaaacagcg aaaaaggtca gggtacagaa tttcatataa tatttcataa aatgtatgat
    39241 agtgactaat ctgtaatatt taattcactt tatagtaata gccaaaacct aatatatgaa
    39301 gtatagaata taaatattgg aggtagttat ggaaatttta agagtagaaa atctaactaa
    39361 aagttatggt aaaaatgaaa caaaagttga tgctataaaa aatgtaagtt tatcagttga
    39421 aaaaggaacg tttatagcaa taacaggtcc aagtggaagt ggtaaaagta ccttattaca
    39481 tttattagga ggagttgaca gaccaactag tggtaaggtt tatataaatg atgttgatat
    39541 atataattta aaaactaagg atttggcgat atttagaaga agaaatatag gtcttatata
    39601 tcaattttac aacttaattc cagtcttaaa tgtgaaggaa aacatattgc ttccagcaga
    39661 gcttgacaat agagatattg atagagaata ttttgatgat ttaatgaaaa cattaggtct
    39721 aattgataga gaaaagcacc ttccaaacca attatcagga ggtcaacaac agagaacctc
    39781 tataggacgt gcattaatta atagaccttc tataatatta gcagatgagc caacaggtaa
    39841 tttagatagt aaaaattcaa aggaaatatt agaattgtta aaattgtcag taaaaaaata
    39901 caaccagaca ctaataatga taactcatga caaaaatatg gcacttcaag ctgatagaat
    39961 tataagcata gaagatggaa tcataaaaag tgatgaggtg atgtagatga atttatatac
    40021 attactaacc attagatact taaaacaaaa taaaagaaga accattgtaa ccataatcgg
    40081 tataatactt tcaacagctt taatttgtgg tataggaaat atatttgaaa gttttatgga
    40141 ttatcaaatg agagaaacta taaagaatga tggaagtttc catgttacat tttatgatgt
    40201 aaatagaaaa aatgtggaat atgtaacgaa atcagctgaa atagaaaaac atgcatttac
    40261 caaacaactt ggatattcta aattagaaaa tagtgaaaat gctatattaa gtataaagca
    40321 atatgataaa aatgcaataa atggatataa agtttctata aaagaaggta gatttccagc
    40381 taaggaagga gaaattgttc taagtgaaag tattattaat cttatagatg ataagttaaa
    40441 aataggagat aagataactt taaaagtagg ggatatgttt gactcaagca agaaaaagat
    40501 tgatagtatg acatttcatg aaggtgatta tatagcaaat gaaaaagata gaacttttaa
    40561 agttgttggt ataattgaaa aacctgggtt tgaacgttat aatggaattg caacagctat
    40621 agcctacttt aatcctatga ataattacaa tgatacaatt aatgtatcag tagcagtaag
    40681 aaatcctaaa gatgtgtata agataagtaa taaaataagt aaaaatatat attcaaataa
    40741 agataaagaa gcagtttctg atatgataaa atacaatgaa catttattga ggttacaagg
    40801 tgctagtaag tattcaaata taaataactc tataaagtct ataattatag cagtaactat
    40861 tttagtaatc atatgtacaa ttgctacagt gtacaattcc tttagtattt ctataaatga
    40921 acgaaaaaaa cagtttggga tattaaattc tataggtgct acatctagtc aaattaaaag
    40981 attagttttt atagaaggta ttataattag tttaattggt ataccaatag gactaatatc
    41041 tggaacagta gctatagacc tattgtttaa aattataaat aagtatttta ctgaatcggt
    41101 tgttactaag atgagtttgc agatagtgta taatccaata ataactattg ttagtataat
    41161 aatagttttg cttacaatct ttatatcaat tctactacct gcaatatcgg cttctaatat
    41221 ttctcacctt aatgtgatta aaaatactgg agagtataaa gtgggcaaaa tcaaaagctc
    41281 aagacttatt aaaatgattt ttaaaactga aggagtactt gcatataaaa atattaggag
    41341 aaatagaaaa aaatttataa taactctttt ttctttaatg gtaagtataa taatatttat
    41401 atcttttagt ggctttacat tacttttact aagaggtgaa gaaattagaa attctcaaag
    41461 aaaatatgat ttgtatttaa ctgcaaagaa cacagctagg tctgtagagg atactataag
    41521 tgaactagaa gatattgatg ggataaaaaa ctttgaattg gcaacaggac aatatgtgtc
    41581 tataagagta agtgaaaata aaataaataa atccaaggaa gatttaatca ggaaatatta
    41641 tcaaaaatat aagattggag atagttatga atatgatttc agtaataatg aattaatttt
    41701 tccaggagat tttgcagtaa aaaacaaaat aaataactta gtacagggct cttttaataa
    41761 agagagagca attgaagaaa atggtgttat attagttaga aaaagtgcat ttgaagaaaa
    41821 tgggaagaaa ggtgtagtag aacttaccaa ctataaagta ggagatacag taaattgtga
    41881 atatcttgat gaaaatgcct taagtaaaaa agtaaaggtc aagattcttg ctataactga
    41941 cgaagaacgt ctaggtcttg gttatcaaaa tatggggtta caatttataa cttatgatga
    42001 agttgctaaa aatttaaatt taaaattaag taaaagcctt atatttattg attcaggagg
    42061 aagcattaag actaagaagg aaatagaagc tttggcaaat aaaaataatt ttaactttta
    42121 tgatgaaagt gcaagcaatg aaagtgaaaa acaagattta aaggttatta aaatatttgt
    42181 atatggtttt atagtagtga tatctttagt gagtgttaca aatattctaa atacagtaag
    42241 tacaagtata aatctaagaa aaagagaact tgcagtaata caatctatag gggttactcc
    42301 taaaggattt aggaaaatga tatatttaga aagttttata tatggtatac tgtcattgtt
    42361 gtttggaata ccaattagta ttggtataat tctaattatg aataaactta tttcaggtgt
    42421 aattgaattt tctccgatta taccgtggac agccatagtt atctgtatag taagtgtttt
    42481 tataataact tttatagcag ggtatatacc aatgagtaaa ttaaataaag aaaatataat
    42541 agataatata agaagagaaa gtatatagat ataaaaataa ttcttaacta tataaaatat
    42601 tattatatca cttaaatgat tttttattta tataagaaat ctaataataa attactagag
    42661 gtatttaact atttagaaag aattggtatt atataaagta agagaatata taataagaaa
    42721 gaatatttga ttttattgga aaacaaaaga aaaagaaggt gattactatt tctcatataa
    42781 ctgcgaagaa tatgtataaa agtttagagg aaagaataaa caagtttcca caaggtgctc
    42841 caccatctga tacattatac aaaatactaa atgtactata tacagaacaa gaagcgaagt
    42901 tagttgctca gcttccaata aagcctttca gagtaaaaac agcagctaaa atttggagtg
    42961 taagtgaaag tgaagcatat agagtattgg ataaacttgc tagtaaggct ctgatactag
    43021 atatagaaga taataaaggg aagaaatata taatgcctcc tccaatggct ggtttttttg
    43081 agtttgccat gatgagaacg agacacgata tagaccaaaa acttttggca gagctttact
    43141 atcaatatat gaatgtagaa gaagatttta tcaaagattt attttattca acagaaacga
    43201 agcttggaag agtatacgtt caagaggaag ttttaactaa tgataatgag gttagtattt
    43261 tagattatga aagggctact catataattg atgaatcaac tcatataggt ataagtatgt
    43321 gttactgtag acatagaatg caacatgttg gaaaagcttg tgatgctcct atggatatat
    43381 gtatgacgtt tgacaatgta gcaaattctt taataaataa taaatttgca agacgtgtgg
    43441 ataaaataga atgtaaagaa ctacttcatc aagcatatga acataactta gttcaatgtg
    43501 gagaaaatgt taggaaaggg gttacgttta tatgtaattg ctgtggatgt tgctgtgaag
    43561 caatggtagc agctaaaaga tttggaaatc ttcatccagt acagacaaca agttttatac
    43621 caaatataaa ccatgaaaat tgtgtaaagt gtggtaaatg tataactgca tgtccaatag
    43681 atgcgataag caaggttaag gaagatggta aagaatatat aaaaattgat gaggatagat
    43741 gtctaggttg tggagtttgt gttagaaatt gccataaaaa tagcattatg ttattaaaga
    43801 gagatgaaaa aataataact ccagcaaatt cagtacatag agcagttctt atggcaattg
    43861 aaaaagggca gttacaaaat ttaatttttg ataataatgc tctggctagt catagagcta
    43921 tgggagctat actgtcagct atattaaagc ttgaaccagc taaaaagatt ttagccagta
    43981 aacaattaaa gtcagtatat ttagataaat tgttaagtat gaatgataaa tagaaactaa
    44041 cttaaaaaag gcaaagtttt agacttatct atatcttaat aaacttatca tttaatattt
    44101 aaatttaatt tttacacata taaaacttat aacttaaata aaaaaaggct atattcaatt
    44161 aatattactt gaatacagcc ttttttagtt atgttaagct atttctttta tttgcttaaa
    44221 tgagttttca tttccatttc caccaatttg tgttacagat ttaaatctca tagttttaaa
    44281 tagttctttt tgattataat ctagtgagtt tcctactagc attacaggag atttagtttt
    44341 tccagccaaa actccaacag ataatccgtc tattaaatca tcttgtttat tcattccatt
    44401 tttagcaaca tataaattgt ctatcttaga atccttgtaa aattctttta ttacttttgc
    44461 attagtttca tttctagtgc tacctgatat tctctgtgga ttttgaagtt tactttctat
    44521 atttttagaa actgtatatt ctccaccgat tacataagat ttatcaatct ttttgttttt
    44581 aaacaagtta tttatttcag ttatattact attttcatta gtaagtatta taggcatatc
    44641 attttgagct gatacagctc caatgcttac tgcatcggct aatccttttt cgccattaac
    44701 tactgaaatc tttgatatat tgctaatatt gttcaattct ttagcaatgt tcatagaagt
    44761 ttgatatcta tcacttcctg aaattctaga tacagagata ttgtttgatt ttatagtatc
    44821 taaagatttt tcatttatag aagcttctcc accaactaca tatacatttt taacttttaa
    44881 tctctttaat tcagccaaag ttttttcatt tatttgagag cttccagtca ataatatagg
    44941 agcgtttttc ttgtatgcaa atggtgttgc agccaaagca tctgcaattg ctgaatcgtt
    45001 tattaaaact gcattttcag cattttccca acctgcttga cttatcttaa tagaagtttc
    45061 atatctatca gttcctgtaa gtgcacttgc atttacttca tcttgatatg taacctcaaa
    45121 tctagcattt ggacttccta agaaaacaat tacgcctcct tttggcaatc taactctatt
    45181 gttctttgta actaaagaat agtttacagg aacaccttta tcatcataaa cagcaaaagc
    45241 tgaattttga ggaagattaa cttctatttt tttatttgcg atatcgtcac caatcttata
    45301 ccattttgca taaccattgg attctagttc acaagtaaaa gatttttctg ctggcaaatt
    45361 agttatagaa tcttcactaa catacgttag tgttccaaaa gataaatact ctgtaccatt
    45421 ttctttatga agtttaatgt cggataaatc acgacctatt acaccaggta tttctataaa
    45481 agcatttgag ttattctcat ccattatttt agtgttaact atataacctg gagtttcatc
    45541 agaaagacta aaacttgctt ttacagagcc aaacatatag ctttgagaag tatatttttc
    45601 atccactaga tagtaacctt tgccacttct ttttttccac acttctttga cactatttga
    45661 tatattgttg tcatctactt tttgagctac atagtataaa cttgctgtct gacctaatcc
    45721 agggacatca tcataagagc tcatatttaa atatgtgata ttattttttt cttttacaaa
    45781 ttttaaacaa ctattacctt tttcagatac aaatctatct tgacctatgt atacgtattt
    45841 atcttcaggt ccattttcta tataaggaga agatactgtt aaagtaccat tatcattaac
    45901 atctactttt atcatatttg aagaagcata aagaccacta ttttctttta aagagctagg
    45961 cattttaact tgttgtggct tactaaatgt tttatcaggt tttatttctt taattttacc
    46021 tttttctttt aatgcagata gtaagatttc ttgtccaatt atttcattaa gttgactact
    46081 tccaccagaa gaaagaactg caactgccat attttcatct ggaagaacta taagattact
    46141 gtggaataat aaagaatctc caccttttgt aagagctttt aaattatact gattaaatgg
    46201 ataagtgttg acacaatccc accctaaacc atatccaagt atagagtctt ctccttcagg
    46261 ccatagacca tttaaatatt ctttgttttc catagctttt actgaagcag gagaaagaat
    46321 accatttgaa tttttcataa atgtttgtgc aaatgtacat agattttctg cacttgaata
    46381 taaaccacct gcacctatag cattaaggtt atcttgtgga actgcatctt cccaataagg
    46441 tacatatgct tttgctagtt ttgaactatc aaaactattt tctggagttt ttgtattttg
    46501 taagttaagt ggattattta tgtatttatc aagaaaatta gtaaatgaca taccagatac
    46561 tctttctact aaaatttcag ctaaagtaaa tccatcatta caataaacag aaaaagctcc
    46621 tggtttagct tttaatcttt gtttttgtaa ttcttttaag aaattatcat gaccataaga
    46681 atcattgtct gctaataaaa tagtgttttt aaaactactt cccatcaacc ctgatgagtg
    46741 atttaataac attcttggag tgatttcctt gtatctatca tcagccattt taaattcagg
    46801 tatatacttt acaacaggag tatctaaatt taattttcca tcatcaacca acttcataac
    46861 agctgttgta gtaaacattt tactgataga tgcgatacta tacatattat ccttatttaa
    46921 gtttttgtta tcctgtttgc tgtaaactcc accattacca gaaatctcaa ttttgccatt
    46981 atcaatcaaa gcatattgag cacttacagc accatacttt gatagcaaag tttggatttt
    47041 ctctttagca agagctttag tttttgtgta agattccgcg tttttactat aatcatgtaa
    47101 ttctatatca gaactttgat atttttcgga attatctttt gtattatcag caaaagtaat
    47161 atttggagca gcagtaaata acatactcaa tactaatgta ctagataata atttagagag
    47221 cctttgctta aacatattac attacctctt tcccttttta ataatttata attagcataa
    47281 cattcttaat tattttaatc aacagattat tatcaataaa aaaagctttt ataagtatag
    47341 ctttttatat aacagagaaa aatataaaaa taatatttgt tgttgtataa agaataaagg
    47401 gtataggtaa tatataaata aaatggagga attttagaca tatgtataag aatataaata
    47461 agaagcaact taaagaaatg atgaaaaatg aaaaggatgt tttattatta gatgtaagaa
    47521 ctaaagatga atttaaggaa tataaaatag aaaatgctat aaatatatct ttacaagagt
    47581 taatcaataa tatagatgaa atttacgatt ataaagataa gaaagtagta gtttattgca
    47641 gaagtggtca tagaagtgtt actgcatgta atttgttagc tgaagaaggt tttgagcatt
    47701 tatataattt aaattcagga ataattgatt atatgagtta aataatgtaa gttatacatt
    47761 aaatactatg tatagctctg cttattaagt aggaatatat ctaaatattt ataagataaa
    47821 tttaggaaag aacgaatttt ttcgtttttt tctatgacag tttttgtagt aaatattagt
    47881 ttttaagttg gaaaaaggta gtagcttttt agctactacc ttttaatatg aacttagaat
    47941 attattaaaa aaatatattt aaagacagaa ttaatttttt aatgaccagt taaattacta
    48001 tcgccgaatt tatcattaaa tggttttata actctattta taggtccttt tagaagtaca
    48061 tttaaacaaa tagacagaat cataaatagt aatagaacta gtatatcttt agttagattt
    48121 ggagcatata taccaccaac tgcttccctt gtagcaccta ttgcataggt aaatggcatt
    48181 atagggttta ttctttggaa gaatgtaggt gtaacttgta tagggaatgt accaccagaa
    48241 gaaccaatct gaacgactaa tagtactatt gcaattgctt ttcctacatt tccaaataca
    48301 gaaactaaag aatacactat aaagttaaat accatactag taaacataag cagaactatc
    48361 aataaaattg gatgttttgc tgttactcct aacaataata tatctccagt tcctattata
    48421 aaaccttgga caagagctat acttaaaaaa gtaaggcctc ttccaaagta aatttcataa
    48481 ggtttatagt ttccatgtac agatgtagac aatagtgctg aaataagtaa tataccaacc
    48541 catgtagaaa gaacactata aaatggtgtc attgcagaac cataattttc aattggatat
    48601 aatttctctg ttttcacctc aacaggttct tttaagaaat tagactttgt caatatatca
    48661 ctttcaagca agctgactaa ttttttcata tcttcaccat tactaatttt gcttaaagta
    48721 tcaatcaaat catccaacat tccttttgct aaaggaagtc tttctcttat caatgatata
    48781 ctttcctgtg catttcctga aagttttaga gaagtagtaa gtatttcttc aacctttgga
    48841 agttttgctt ctgccttatc taaaacagta attatatcat ttgctacttt tatactgttt
    48901 gcaaatatac tatttatagg ttttgatatt tttgagtcaa aattatttaa aatatttaaa
    48961 ttaattcttc caataccatt tgatagactt aaaacattat tcaaagcact tatagatggt
    49021 tgttgtccag atataacttt gtttttaata tcatttaatg ttgatataga tgaatctatt
    49081 ttattgctag agtcttcaag attatctatg acatcatcca atctattatt tgatgtaagt
    49141 tggtttaatt tagttaaaaa atccaccaaa gtatcattta aagattgcaa attagaaagt
    49201 ttttctgata aattatctat caacttaggt acatcttcac tgccactatt tactgcatca
    49261 attaaattta atgtaagaga agaagcagaa gatgaaaggt caaccattaa atttaagtca
    49321 gacttaataa gaggagaaag ttcgtctaaa ttatcatttg tatcctctaa gaattttttt
    49381 aagtcacttg ataatagttt tgtatcattc agagtctttt taattgtagg taaatcagat
    49441 ttaatatctt taactgtatc agataatttt gaagtagcat cagaagctaa atcaacagtc
    49501 ttatttatat ggtcaaatct cttttgaagg tcaataaggg aattttcaac ttttgtcaat
    49561 tttggaagac tgtgttcaat tcctgcacca actccgtttg atataccaag aacgatttca
    49621 ctaactgttt ttactatagt ctgattaacc tgtagttgta ttgtagaagc acctttatct
    49681 gtaatctttg gtgcaactgc atttattttt tcattaacag tatagataat ttcgcctttt
    49741 ttaacatcat ctgtaattaa agatgtcaaa tcttttgtaa aattttttgg aatcttaagt
    49801 gatgcataat aagtaccttt tttaacacct tcaagagcag ttttttcatc tacaaactgc
    49861 caacctaaac tcttgttatc tttgagttgt tcaattaatt ggtctcctac attaatcttt
    49921 tgtcctgcta tttcatttcc tttatcttca tttgttactg ctacaagcat attagcagtg
    49981 cttccataag ggtcccaaga tgcctttata ttgaaccaag catacagaga tggtaaaaaa
    50041 gcaagaccta aaactattac aagtacggca taattgccac gtatatcttt taggtcactt
    50101 ttaaaaattt ctaatatatt ttttaaaccc tttgaaggtc ttaaattctt gatattattc
    50161 cagtttaaaa tttcttttaa actattagtt tttttcaaat gtctcacctc ctaaaatatt
    50221 ttgataaaga aaaaagttag atttataatc aattttaaat tatattactt tttacacaaa
    50281 tagtaaagtt aaaattttgt cttaatttta atacatatat attatatata atagtatgct
    50341 ataaatatat ataaatatag gaaataaaaa caaataattt ataaaatata tggtataaaa
    50401 attaataata aatcaaatat ttataattga tataatcaat taaattagcc attatgatat
    50461 acttttggta atgagaaaat tctatgaagg gatgattgaa tgaggtattt taaaaaatac
    50521 ataaaaaaat atatagtatt atttctaaca gcagtatttt ttttaacatt ggaagctttt
    50581 tgtgatttag ctcaaccaac tataatggca aaaataatag atataggggt ttcaaataag
    50641 aacttagact acatactatc aacaggagct ataatgatag gtataacttt tttaggtgca
    50701 atatttgcaa taataagaag tgtaatctcg gcaagagttt ctcagcattt ttcttatgat
    50761 ttaagacagg atgtatttga aaagataaat acatactcat taaaacaaat agataaattt
    50821 ggtagagctt cattaataac aagaattact aatgatatca atcaaatgca acaattcgtt
    50881 aatggaatga tgagagtttt tataaagtca ccaataatgt gtataggaag tttaattata
    50941 gctataaaat taaatttaaa gatgtcaata attattctaa ttgcagtttt aattatattt
    51001 attataatat tgcttaatat gaagataggg tatagattat ttaaaaatgt acaagaaagt
    51061 acagataaag taaattcaaa attaagacag tttttaagtg gaataaaagt agttaaacta
    51121 ttttgtagat acgattatga gaaccaaagc tttgaagagt taaatgatga actttttaaa
    51181 aatagtagaa aagctacaac tatgatgtca ttttttagac cgacaattac aataatcatt
    51241 aatgtttcca taataataat attactgatt ggaaggcatt taatttattc aggagaaatt
    51301 aaaattggga ttatagttgc ttatgtaaac tatatgacta gaatattaac atctcttata
    51361 atgatttcac atgtttttaa tgtttttgtt agggcaaaag catcttatga gagaataagt
    51421 gaggttttgg ttgatgagaa tgaagaagta attgagagag aagaaagttt agatgttgga
    51481 gaaaaagatt taaagtttgt aaactgtgat ataatttttg aaaatgtaga tttttcgtat
    51541 aacaaaaata cagatgaaaa aatattaaaa aacgtatcct ttaaaataga agaaggtcaa
    51601 aatattggtg tgattggtca aactggttca ggaaagactt ctttagtaaa tttaattcaa
    51661 aaattatata taattgataa aggtgatatt aagattggag gacgtagtat atacagtatt
    51721 tctgaagaaa gtttaagggc aagaataggt gtagtatttc aaaatagtag tgtgtttgga
    51781 aaaagtatat tggagaatgt agttatggga aatagagata tatgcaaaga ggatgttgta
    51841 aaatcatgtg aaatagcaaa tgcaaatcaa tttataacaa aactcgaaaa tacttatgat
    51901 gaagttttat tccaaagggg taaaaattta tcaggaggac agagacaaag aattactata
    51961 gctagagcta tagccaaaaa accacaaata ttaattcttg atgattgttt tagtgcaatg
    52021 gatattaata cagagacaat tataagaaag aatataaaag agtatataag taatttaaga
    52081 gaaaaggaaa gtaagaaatg tactgtaata actatttcac aaaagattag ttctataatt
    52141 gactgtgata agattttagt cttggatgag ggtgaggtgg ttggatttgc tacacatgaa
    52201 gatttactgg ttagttctcc aatttataaa aaaatttttg cgttgcaaaa tttagtaagt
    52261 gaagatgtaa attaaggagg aagtatattg aatttaagta ctggaaatca gaaggtcagt
    52321 agattaaaga aaaaagaaaa gcctaaagat tttaaaaaaa cgtttaaaag aatttttatg
    52381 tactttaaaa aagataagaa gattactaga tttatttttt tgctagtaat aattgattca
    52441 attatatcaa ctgtaatacc atatactatt ggaaaggtag ttgacataat agatatcgat
    52501 acttattcaa aatccatttt cttaaaagga ataattattt tactttcatt ttatataatt
    52561 gagttttcag ttaaactgtc taagggaata tgtacagcta gattatctca aagtatagtc
    52621 agagaaatga gaagtaattt atttttaaaa tttaaaaatc taccaataac cttttttgat
    52681 aaaagttcta ctggagatat tatgagtaga gtgacaaatg atgtcgataa tatcagtaca
    52741 ggaatatcaa attcattagt acagttaatt acaggaatct taaacatatt gatgactttt
    52801 attatgatgt ttgtattaag cccaatttta accttttgca gtgtaatatt aataccgata
    52861 gtaatgtttg tgagtaattt tattgcaaag agaactagag ttttttttaa gcaacaacaa
    52921 gttgagttgg gaaaactaaa tgcacatatt gaagaaatga tatcaaacat aaatgtagta
    52981 aaatctttta attatgaaag aaaatctgta aatgatttta aagaagtcaa tgatagactt
    53041 ctaaatattt cccttaaatc gcaaatttac acttctcttt taatgcctat tatgaatgtg
    53101 attagtaata taggatttgc aactatatgt attataggag gcattcttgc atctaaagat
    53161 attataacca taggggttat agctagtttt ttaagttatg taagacaatt cacaagacct
    53221 ttaaatgaat tagcaaatct atttaataca ttactttcag ccatagctgg atgtgaaaga
    53281 gtttttgaaa ttatagatga acaagaagag caagaagtgc ataacatgtt taaaagtgag
    53341 attaacgata ataaagatgg taatttaaac tctcattttg taaagggaga aattgagctt
    53401 aaagatgtaa gtttttctta tgattcaaaa aagaaaattt tagataatat aaacctaaaa
    53461 atcaaatctg gcacatcaaa tgccatcata ggagagacgg gaagtggaaa aacaactatt
    53521 ataaatctaa ttacaaattt ttatcatata gatgaaggaa gtatattttt agatggtaag
    53581 gatatatact ctattaacag aaactatttg agaacttgtt ttggagtagt tccacaagaa
    53641 ggttatatat tcaatgatac agttatggaa aatataaggt atggaaatct ggaggctact
    53701 gatgaagaag ttatatatgc tagtaaaata gcaaatgcac ataaatttat agttaaaatg
    53761 cgtgaaggat atagtactgt attatcggat aggggtgaag atttaagtga aggtcaaaaa
    53821 caattaataa gtattgctag agcagtatta aaaaatgctc caatacttat tttggatgag
    53881 gctacaagta acatagatat agcaacagaa gctaaaattc aagaagcaat aaataattta
    53941 acttatggaa agacaagtat aattatagct catagattaa gtaccatatc aaattgtgat
    54001 aatataatag ttttagaaaa tggtagaatt gtggaatcag gaaatcataa agaattgata
    54061 aaattaaagg gacattatta tagaaacata ttattgacac agggaaaata agtatttaga
    54121 aaaaactttt aaaaaaattg tttaaaggta tatggtgcgg gtatatatag actaacttaa
    54181 taaataaaaa atttaatttg atatataaaa tttaggagga aagtaattat gaagaaattt
    54241 gtttgtacag tatgtggata tatacatgaa ggagatgctg caccagcaca atgtccagta
    54301 tgtaaagttg gagctgataa atttgaagaa atgaaaggcg aaaaaaattg ggctgatgag
    54361 cacagaatag gagtagctca aggtgtagat gaagaaataa tcgaaggatt aagagctaac
    54421 tttgttggtg agtgtacaga agtaggaatg tatttagcaa tgagtagaca agctgataga
    54481 gaaggttatc cagaagtagg agaagcttat aagagaatag ctcttgaaga agctgaacat
    54541 gctgctaaat ttgcagaact tcttggagaa gttgtagttg cagatacaaa agaaaactta
    54601 agagttagag ttgatgctga gtatggtgca actgatggaa aattaaaatt agctaagaaa
    54661 gcaaaagaat taggattaga tgctatacat gatacagtac atgaaatgtg taaagatgaa
    54721 gctagacatg gtaaagcatt cttaggatta ttaaatagat attttggaaa ataaatttaa
    54781 tataaagtag attttaggag tatctcactt acaattttta aagttatgag tgagatattt
    54841 tttaattatt tgtactttat tttttatttc taacgattat atttttaata atagagaata
    54901 atttaatagt aataatgcac aacttttaaa aataaatgat taatccaaaa ataaatttga
    54961 ttttagaaaa gtaaattgac atgtaaagaa gaatatatta aactaagtat aaaaaatttg
    55021 ctgttttgta aaggtggtgg ataatctgat aaagtatata cataaaagtt atgtagctat
    55081 tgttgtttta catgaaatct atgggattaa tagctttata gaagatgttt gtcaaaagta
    55141 ttataaatat ggatatgatg tattttgtcc tgaacttctg ggagaaaaaa gagtttttcc
    55201 atattcaaaa gtccaagagg catatgattt ttttataaat aatgttggtt ttgatattta
    55261 taaaaaagtt gaaaagctta taaatgattt aaaatcagag tataaatatg tatttgttgc
    55321 tgggtatagt gtgggagcaa ctattgcatg gagatgcagt gaagattctt tttgtgatgg
    55381 cataatttgt tgttatggct ctcggattcg agattacatg gatgtaattc caaaatgtcc
    55441 taccttatta gtatttgcaa agcacgattc atttgatgtt gattctattt cttaccaact
    55501 ttctaggaaa caaaatatcc aaattgagat tttagatgca aatcatggtt ttatagatag
    55561 atattcaaat aattattctg atattcaaac aaaagtattt actattaaag aaaaaagttt
    55621 tctgtctaaa tatttagcat gatttagatt attataaaat atatagaaag atttaatt
//
LOCUS       DBGJFU010000012        50237 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.012, whole genome shotgun sequence.
ACCESSION   DBGJFU010000012 DBGJFU010000000
VERSION     DBGJFU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.012"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            404..895
                     /locus_tag="ACOIAS_000712"
     CDS             404..895
                     /locus_tag="ACOIAS_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGZ2700433.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
     gene            1424..2296
                     /locus_tag="ACOIAS_000713"
     CDS             1424..2296
                     /locus_tag="ACOIAS_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700434.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            2293..3582
                     /locus_tag="ACOIAS_000714"
     CDS             2293..3582
                     /locus_tag="ACOIAS_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2700435.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            3512..4156
                     /locus_tag="ACOIAS_000715"
     CDS             3512..4156
                     /locus_tag="ACOIAS_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2700436.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(4423..4647)
                     /locus_tag="ACOIAS_000716"
     CDS             complement(4423..4647)
                     /locus_tag="ACOIAS_000716"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2700437.1"
                     /translation="MKPNYCGMGIGKEIISLGLQECRNKYSTKPIVLNVRTWNMRAVK
                     CYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(4644..4886)
                     /locus_tag="ACOIAS_000717"
     CDS             complement(4644..4886)
                     /locus_tag="ACOIAS_000717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700438.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIG"
     gene            complement(5886..6713)
                     /locus_tag="ACOIAS_000718"
     CDS             complement(5886..6713)
                     /locus_tag="ACOIAS_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HGZ2700439.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            7333..7788
                     /gene="smpB"
                     /locus_tag="ACOIAS_000719"
     CDS             7333..7788
                     /gene="smpB"
                     /locus_tag="ACOIAS_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGZ2700440.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            7946..8295
                     /gene="ssrA"
                     /locus_tag="ACOIAS_000720"
     tmRNA           7946..8295
                     /gene="ssrA"
                     /locus_tag="ACOIAS_000720"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            8410..10437
                     /locus_tag="ACOIAS_000721"
     CDS             8410..10437
                     /locus_tag="ACOIAS_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGZ2700441.1"
                     /translation="MSIDNNFNHVAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIIKSVYEEYDFLPEEERDELVIVDLKENHDINLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNNVKEIIGKFKFKDEINQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNKMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEILFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKINPSAIVNVYGQELLDESFAICQADMLMKGQNPDNIRLGNT
                     LTQDRFNGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIENDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEAKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEQIKEI
                     YEKFEESENSKIFDNEDFGYRKVTIERPLKLSFKVNEEAIENVKNTTQFINLAVSKKK
                     DIEVKEKEEAEGKIKQDKLIKLLESFDNTLEYMDRDKFIKDLKAKSKEFDIQLSAGLI
                     KAIVNTIGVRNEDAEVCKDSKGNIESDSSLKDTESIGLKDDVYEYFEKEVKPHVNDAY
                     IDESSINNIGYEIPFTRHFYKYEELRSFAEIMKEVESLESEIALKIRKVLG"
     gene            10437..11828
                     /locus_tag="ACOIAS_000722"
     CDS             10437..11828
                     /locus_tag="ACOIAS_000722"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGZ2700442.1"
                     /translation="MGYRYRSDDELKDSGVEWLGKIPKDWEVRQLKRVLSDRKENNEP
                     IKTSNILSLTIEKGVIPYSEKGTGGNKAKEDLTQYKLVYPNDIVLNSMNVIVGAVGLS
                     KYFGCASPAYYMLYKINANDDIRFYNYIFKSKLFQDSLKGLGNGIMMKQSETSGKLNT
                     IRMRIPMEKLSSVKIPIAFNLDQEKIANFLDEKTSQFDSIISKKEKLIERLEEAKKSL
                     ISEVVTGKVKVVKTDDGYELIKRSSEEMKDSGVEWLGEIPKEWDIKRLRFLGKLQNGI
                     SKSSDSFGYGYPFVSYSNVYRNIELPHIVEGLVNSTEEERKIYSVEEGDVFFTRTSET
                     VEEIGFASTCMKTIDNSTFAGFLIRFRPNDKRLYKGFSKYYFRCDLNRKYFVKEMNLV
                     TRASLSQNLLNNLSVVLPKYEEQVEIYNALELKVSYINLSINKLKYQIEKLKEAKQSL
                     ISEAVTGKIEILD"
     gene            11890..13161
                     /locus_tag="ACOIAS_000723"
     CDS             11890..13161
                     /locus_tag="ACOIAS_000723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700443.1"
                     /translation="MDKNIFQININTDVLIRLRNQINEEYYISYRKEFYDKNNKKHRA
                     WDKICAIMDRLDDTVDYLNKLKLNTGKYRRSAFDFFEFMNQASVVMDCITHLAKIFEV
                     PYEEVETSTEIFNELGKEGKGTDKKYFEYLRSLCSVHPMETSRHKIYQENSFECSPYV
                     VWNDKSIWTNDASDLYAVVYTSKDNDSFKRIHIYMNQIFEYVKRRVNFIENIIEHIEK
                     YQQEIIEIFRNTPIKKEEDFESYIDYLRNLKKEIEIRYGNDSFHELNYLIELFDLPLS
                     NPDNEEKMNLYKNALKYAIKFEHNSIQNMTNEGYKNNGILYPQVNITTTLLEELISLN
                     SGSEERRKYRYAIEKTSYLSYDSCWSDREWAYDQIDDMMPFLERYVTFDKAQSDFEHY
                     VLVKLALYLECLENECLINKNVPNDKKFRIK"
     gene            13196..16177
                     /locus_tag="ACOIAS_000724"
     CDS             13196..16177
                     /locus_tag="ACOIAS_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HGZ2700444.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVDDLFRFL
                     ENTQKELLDEFKYNRGSNYKKAFIDLLNSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKKDRNPKETLFGFNQRVLVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     RGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKGNQRAIFPRFHQLDAV
                     RKIVEDLKINKVGKNYLIQHSAGSGKTNTIAWTSHRLSSLHDEENNAIFDSIIVVTDR
                     KVLDKQLQNAIYQLEHKNGLVVKIDEDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLDKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKVIDAENEKNEKSSIDKMN
                     ELIEKRTNLGNISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPISVYYKVGKKIIDNPEFDKGEAKRAINKFVSLNEHNIRQKVETIVDDFVDNR
                     SMWLEGKAKGMIVTASRLHAVRYKLAIDQYIKEKGYDMKALVAFSGTVKDGDDEYTEV
                     GMNKEVAPDITDSNLPNIFDKNEFKLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKQTFILDFQNSVEDIQDAYKPYFEMTNIDKVTDPNVVNDLWYQLHEY
                     AVYTDEEVNDFTVLFYKEKRTSSQDAKMNNLIDKAVERYCYLEEDDEKKSDEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLKKLPKKSKQRIDLDDMLSLDYYKLQKL
                     GLDDKKGTDISLYNGEGELVGISETATSRVSEADEEYLEDIIKKINDVFGVGLTEDDR
                     IMINTYEEMFKNDKDIMDIAKANSYEDLVSTFGKKYFKRGIVKTKNRNDRLVKEILTN
                     NNLQNYMIHYLAEKIYEEANR"
     gene            complement(16236..16406)
                     /locus_tag="ACOIAS_000725"
     CDS             complement(16236..16406)
                     /locus_tag="ACOIAS_000725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700445.1"
                     /translation="MNNDKPFIEFEEEVDFILTECKKEGVSIDRDTIELILDLDLKFL
                     ELKGIATPLEDE"
     gene            16687..17022
                     /locus_tag="ACOIAS_000726"
     CDS             16687..17022
                     /locus_tag="ACOIAS_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700446.1"
                     /translation="MIKLWKKEDLNLLLEYPKEVVENVDNVINILDENYGCNRKLTDD
                     GGYVCIIEDIKEVENLKSNILKGLVEEFSDVIYEDEVNTYNSTLYLLSSDYSVTVISK
                     NEETEYLLK"
     gene            17086..17223
                     /locus_tag="ACOIAS_000727"
     CDS             17086..17223
                     /locus_tag="ACOIAS_000727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700447.1"
                     /translation="MNKKRTPAKRVDIVLLKLVKERSVLYETRKISNLYDAYRLAKNF
                     L"
     gene            17252..17437
                     /locus_tag="ACOIAS_000728"
     CDS             17252..17437
                     /locus_tag="ACOIAS_000728"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGZ2700448.1"
                     /translation="MFRYKELASSIEVVSIGTVNLSMVYPREIFKVAILTNSSEMICF
                     HNHPMGNTDFSKEDSYN"
     gene            17684..17977
                     /locus_tag="ACOIAS_000729"
     CDS             17684..17977
                     /locus_tag="ACOIAS_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 family protein"
                     /protein_id="HGZ2700449.1"
                     /translation="MFKKENRYMTKEIAENLPIEIAILLWNLIDDLTTEKDYLQLFEI
                     NPIGLGVVEIVHKQEVPEYEARIYIQNDKIKDKLKIYVIDNVEYRTMMFSNEY"
     gene            18004..18600
                     /locus_tag="ACOIAS_000730"
     CDS             18004..18600
                     /locus_tag="ACOIAS_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700450.1"
                     /translation="MINNKSEIINKLFFNELKELIDKYNNIDDKTVDVIEKIFTCIED
                     EEIKNYLIRDNCKLQELVNTYNKLEDLNIDEAIFFAWYNLTINTISIDKASEYYDDLT
                     TSNYIEVENHIIYTNERDLRKYAEDELETMLSMEHHIDRLLHKDMLIDMWLNNTSKEE
                     VIEEILMSDDIEDILNLSPEYAFTLANGIEYRYSEKEE"
     gene            18753..19022
                     /locus_tag="ACOIAS_000731"
     CDS             18753..19022
                     /locus_tag="ACOIAS_000731"
                     /inference="COORDINATES: protein motif:HMM:NF020345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGZ2700451.1"
                     /translation="MTNNNNNCDYKGIYADMVEVLGEEIVIKLHKYYRGQQITFPMKL
                     YSIEYVERYIVKNYRTKTIKEMCRELGYTEGWIKQLINKNRLNSK"
     gene            19048..19290
                     /locus_tag="ACOIAS_000732"
     CDS             19048..19290
                     /locus_tag="ACOIAS_000732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700452.1"
                     /translation="MILTVLAILMGTGIFFLINSMLDITYFGCGPIGFLWFGCFIVSY
                     AILTSLGGIVLGLLKWIVIGLIILFVIGLIANKIND"
     gene            19496..20629
                     /locus_tag="ACOIAS_000733"
     CDS             19496..20629
                     /locus_tag="ACOIAS_000733"
                     /inference="COORDINATES: protein motif:HMM:NF012938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGZ2700453.1"
                     /translation="MQDFDFISETIKKFQSDLENMDKVNILVVGKTGVGKSTLINNIF
                     REELAETGIGRPVTQHLKKISKEGLPINLYDTKGLELEEDVQETIKKEILDEIENCNR
                     NSLERNDKSELMHVCWYCINTSVARCEETDIKWINELSEKIPVIVVLTQGFPRKKARE
                     LKSSIDDLNLNCKNVIPVLAEQLVEEEDEIVIKSYGLDKLVEVTYQVIPKEVKKAFVN
                     VQKVDIKKKVEESRKWVLGYVGGSGAIGFSPIPFSDAPLLATAQVAMISHITVIFGLQ
                     VDKALLTAIVSSIGGVTSATLLGKTLVSNILKFIPGAGTIAGGAISGSVAALTTTALG
                     MAYISVLEYILNENMKGNEVNNTEIAEKMKDAYKDQLGMLSKK"
     gene            21336..21638
                     /locus_tag="ACOIAS_000734"
     CDS             21336..21638
                     /locus_tag="ACOIAS_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700454.1"
                     /translation="MAKQSKEELFEKETKYCKIIRSVTVEDGDERFAIEKIYIKSLQR
                     YEVRICLYRDCRDRINKLIPRPVDLTMDKFVALILVGLKAGVLDDDFKQELIKGLA"
     gene            21986..23347
                     /locus_tag="ACOIAS_000735"
     CDS             21986..23347
                     /locus_tag="ACOIAS_000735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700455.1"
                     /translation="MKGNINKKDIDLSKITFDKFLEDEGLQNRERDYLYVDDYDNTLD
                     TQEFKLHIIEENTDNTYIDKDKYWYLDLDDSLFEYEEDEDTIAKTLYSLIDIRIKFLK
                     HVEYRKYINLGVIDEEYFDEIKSFLKFYIVAKTKKNELGNSNLKQMAIDIFDKERFED
                     DISDDDMENISEWIGEDQNFKMFYDKVCRDLSNSTQDKEILKLAGIRNNKVKHYKGRK
                     ISRKCKLELKSLTKMWEYETKLTHDANKAHKKIKSNMLEKMTDKNYKNIYEYLNNEII
                     NSNEEYKNIYFYSLELKLANELRKCTLKNLNKIKDIENQVKFISYTIAYIHIVPVLTI
                     REKLSDKLFNILSTNEGKDIEKFTKEVSLIMRIINIECLELLNIVSKNNYYKNFSEKE
                     LEEFKSIYNINNGRKSFENKYKIKVDYNIDDVRKYGEVIKAINIESKKKIVDRIKKKR
                     KSN"
     gene            23537..23854
                     /locus_tag="ACOIAS_000736"
     CDS             23537..23854
                     /locus_tag="ACOIAS_000736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700456.1"
                     /translation="MTNFKSVKVELILLIECEEGNRNELEGMIDEGVLNEKEYSLTIL
                     GSTEYEDNARAIYILMNTGGSYEKNLQRLSRLHLKIENLLKDTNVKYRGISLVPNIVK
                     WDK"
     gene            23866..25629
                     /locus_tag="ACOIAS_000737"
     CDS             23866..25629
                     /locus_tag="ACOIAS_000737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700457.1"
                     /translation="MSKAINREELIKLYEEIGLNVLDNNTVMNSSNKKICNFLVNIIE
                     QRVIDGKMYLCINAMGINGELLSEVILDSNTINRTTWVIENWGFKYSIDKKYINDFIE
                     LIQHLCMNIKTKYIYDHTGWIYQDNKWIYLHGGGVIGGSDVQVELDESIDNYILPDKI
                     SDINKACEMSLKLVDLIEDDKGIIFSSLVYLSPLIEIISRKIKPPENIVWTYGKTGSR
                     KTAVSKVVLSHFGDFNKKIPATFNDTITAIELKANKLKDSLYLCDDFAPKQDFRDKKI
                     QDSKAESIIRMYGDRVGKGRSSNKLDIKDAKRPRGMILITGEDVICGESSNARLLSIE
                     LNRSSVDLNILTELQNNSHLLGEAMRGYIEWLLSEMGNIDELSNILLYNFKSYRDEIR
                     EQYKDDIHGRTIESSAWILVGFSCMLNYIRDKGVIDKYEDDEYMDRAIKVVERLIEEQ
                     IKLISRNSPIDIFLNTLKESINSKSIRIATLNDQNQVIEDKLEYICGYKDKEYYYFYT
                     DKVYSFIEFENKKRGIHLGISQRQLVKQLRDSGIVKVDSNNDSSKKTIHTKDASDSTG
                     YTTIRPRMLQIERSIIENFQI"
     gene            25692..25847
                     /locus_tag="ACOIAS_000738"
     CDS             25692..25847
                     /locus_tag="ACOIAS_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700458.1"
                     /translation="MTETYKISIGRKDLYSKDYKVVERTDYEKRHTVNISRSLVDILS
                     ILVKEDR"
     gene            25934..27499
                     /locus_tag="ACOIAS_000739"
     CDS             25934..27499
                     /locus_tag="ACOIAS_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2700459.1"
                     /translation="MTGKKAVVYARFSSDNQRDESIDAQLRAINEYADKNNIKIVNQF
                     IDRAKSATSDKRPEFQNMIKFCEADTTGISMVIVHKLDRFSRDKYDSAMYKQKLKVKG
                     IRVVSVLENLDNSPESLILESVIEGMAQYYSANLAREVAKGQKENALKALHNGGDAPL
                     GYDVAHDKTYTVNEEEAQAVKIIFDRYIKGYSYSKIIDELNILGYKTKRGNKFGKNSL
                     HGILNNEKYTGVYVFNKTQRKGVDGKRNGHKQKSEEEIIKIEGGMPQIIDKGVFKQAQ
                     DMIQKRKKTPGAHKATTTYLLTGLIKCGECGHAFQGNKRKDSYGNEYVSYRCGCRKQK
                     RECNNREIKRDYLEEFILQELEKNILNDEAIPILSKALNEKLNDKNESNAELLKNLEQ
                     KLDKVNKEISNILNAIMNGIVNSMLKDKLDELEQVKFNLDLKMNELKIENKVVDGVGV
                     TEDQIRGMFSRFKEFVLERNIPECKKFIGDYIKEVIVYKDHIEVIFNVVFCFVDNDIT
                     HDLHIITERGKIT"
     gene            27645..28937
                     /locus_tag="ACOIAS_000740"
     CDS             27645..28937
                     /locus_tag="ACOIAS_000740"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HGZ2700460.1"
                     /translation="MADIFTVLNYQGSKKRLLDFIYENTQEYIDSDKAFLDICSGTSA
                     VAYNMKRHFTVYTNDSENYAYQISNSIVKNNERYNFSQSDEMIIREKYNINLQRLNNI
                     FNDMVNQEDMLLEQEDADGLIELYSNCPCIWKNNVDIEIEGNTIQSIYDLRKCKRDIP
                     NVLFTLYYSSSYFGIKQSMEIDSIRYAIEFIESEITKSILFTSLFFAMKECVFAKDGH
                     MAQPLNMETGKKRMLRLKARSIYDIFIDKLKEFNTEEFVLSQKENKCFNKDFRTLLET
                     EESIKDEVGFIYIDPPYTDMQYSRYYHILNTVVLYDYPEISLSRGTISKGIYREGRYQ
                     SPLSQRKHALNEIDFLFSFCKENKINVALSYAYPIDTEKQATNRYTASIDDLINVAKK
                     HFGDNVKVVHEEYSHANNRNSDKKNVLEYLIMGIHNKE"
     gene            28940..30559
                     /locus_tag="ACOIAS_000741"
     CDS             28940..30559
                     /locus_tag="ACOIAS_000741"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HGZ2700461.1"
                     /translation="MDKINKLKERIANIEATNKNDIYSSNLYWSQKPFNICDEIIEHL
                     SEDGDIIFDPFMGSGVTVIEALKKEYKRNAIGCDINDAPIFIVKTLLEKYNVPKIEKD
                     IKELNNKLSKLSTYYSTKCRECGEVVTISKTIFDKENRNAKCELKSIHYKCKECKNDF
                     KGEPNETEISKFFIDEELKNIDDFQFIANSRLAVGEGDTVRTIFSNRNLIVLDKILGH
                     IDEYKNSESYEPMKYILLSILHLSKITDTHSNSQWPLWIPKKDCVEKNVVELFIKNSK
                     KTIKALKLASKYLCENRENVELIKNLDRGKFKLIKDGVQNIEDDVIPNEFVDLIITDP
                     PYMGQVLYSEYMQLYKPFMDLEFELENEIVISSAPARDKGKVLYFDMMNSAFENISRV
                     LKVGKYMCMYFHDSNLDVWDNIIDIMTNNNLKYMGQIHVSKNKNTLKNILSPKKSLNG
                     DCIIFFKKDLNINYDINEVDNIEDKINNIAQAIIDIKGYASTPELYDNGALEYIISNG
                     KLKEISKKYKDLTSIFEQRFNWDKERGVWTV"
     gene            complement(30684..31835)
                     /locus_tag="ACOIAS_000742"
     CDS             complement(30684..31835)
                     /locus_tag="ACOIAS_000742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700462.1"
                     /translation="MKKLTIRLKDDFHKDLLDLVAFKNDSINSYVINLIKNDLSKNTN
                     LLRSYDNMIRDNITNVLITQKINELKAIQDGIFKEYKVNDILSNSKIYEVMTANSLNH
                     ILIPGHSGSRDAKDEFGNEFEYKHFKKSSSNHSWTFNDFSDTSIAKLKKAKAVIFAHI
                     NDVDFPYPGKLDWYYSIPGDIMCEYLLKYTQSIQNSRKMINVSPNQLESRLNASKIIP
                     SAQNGVYDKYLSAIYKASSELEELTGVKNLLTSNKFWELIVALELNHQVNPEQGGREG
                     AHDAYDSEGNPYEYKVSKNHAWNFQDISDNVLNKYYNDKAIILAVVDKENIEVEHIYS
                     TEPADVVPLLRKKLQEKINRCLEKGSPIRRLQVSLSKSDLTRVNAKKLY"
     gene            32341..32556
                     /locus_tag="ACOIAS_000743"
     CDS             32341..32556
                     /locus_tag="ACOIAS_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HGZ2700463.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            32556..33035
                     /locus_tag="ACOIAS_000744"
     CDS             32556..33035
                     /locus_tag="ACOIAS_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HGZ2700464.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            33041..33271
                     /locus_tag="ACOIAS_000745"
     CDS             33041..33271
                     /locus_tag="ACOIAS_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700465.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(33387..34553)
                     /locus_tag="ACOIAS_000746"
     CDS             complement(33387..34553)
                     /locus_tag="ACOIAS_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2700466.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(34543..35295)
                     /locus_tag="ACOIAS_000747"
     CDS             complement(34543..35295)
                     /locus_tag="ACOIAS_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGZ2700467.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(35387..35875)
                     /locus_tag="ACOIAS_000748"
     CDS             complement(35387..35875)
                     /locus_tag="ACOIAS_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700468.1"
                     /translation="MNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRMP
                     SLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDIK
                     TDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDEDE
                     EY"
     gene            complement(35948..36073)
                     /locus_tag="ACOIAS_000749"
     CDS             complement(35948..36073)
                     /locus_tag="ACOIAS_000749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700469.1"
                     /translation="MNKSYMLKYTYKSPTNLFHGDKKYTLTYTKRVMSNLVYDMG"
     gene            complement(36130..36900)
                     /locus_tag="ACOIAS_000750"
     CDS             complement(36130..36900)
                     /locus_tag="ACOIAS_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700470.1"
                     /translation="MIILQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRI
                     PSIDTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISN
                     LMEDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPD
                     DIKEIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENI
                     TIKNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            37038..37238
                     /locus_tag="ACOIAS_000751"
     CDS             37038..37238
                     /locus_tag="ACOIAS_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700471.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            37242..37556
                     /locus_tag="ACOIAS_000752"
     CDS             37242..37556
                     /locus_tag="ACOIAS_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700472.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            37578..38321
                     /locus_tag="ACOIAS_000753"
     CDS             37578..38321
                     /locus_tag="ACOIAS_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700473.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(39027..40940)
                     /gene="drmB"
                     /locus_tag="ACOIAS_000754"
     CDS             complement(39027..40940)
                     /gene="drmB"
                     /locus_tag="ACOIAS_000754"
                     /inference="COORDINATES: protein motif:HMM:NF038324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DrmB family protein"
                     /protein_id="HGZ2700474.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            complement(40942..44469)
                     /locus_tag="ACOIAS_000755"
     CDS             complement(40942..44469)
                     /locus_tag="ACOIAS_000755"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HGZ2700475.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            complement(44487..47105)
                     /locus_tag="ACOIAS_000756"
     CDS             complement(44487..47105)
                     /locus_tag="ACOIAS_000756"
                     /inference="COORDINATES: protein motif:HMM:NF038316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DrmE family protein"
                     /protein_id="HGZ2700476.1"
                     /translation="MLTTNIHYILDNDICEIYFNKQRLTNSKYYQDNINVLNYIINEK
                     NNNLIINYNDKYIYAIYLLNIAINSYLNNTLNPNNHILTKLDKGMPLLYKGQIVIFKN
                     INNTHITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQTKNVLSSLG
                     FSDLKNMTGVINDSTLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCSYISSTGAE
                     TDYPGNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHINDFERILNRK
                     RIESVNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQKLWLDKLI
                     NKNICTTSVDSNISDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKILENTPFNL
                     DFLEETIKLLNLNIATPSAKLENIKYLISSTSPDLELSALFDDVIVKFERIINELSDN
                     NTKFNTLNKLVSSYKYKTFTPNTPVILLQKQYEAIILKNLLKSRNINLQVISAEKFKD
                     SKTIKNLIMFGFYDLKYLDILNSDLIINITLYLYNSELSKYNYYMNQINSARNKIEKN
                     NKIYNLLDIKPFKPTYETNKLEAYSTDSPISDDDSMDEYISNYKFDINLNEIILSLSI
                     NQGIYNSKKEHVTSFMISDNNEYIFCTKKAIAYSIDWDKKSVRKKKLSEAKVNDWFLF
                     VDDYLNEEGELVINVIQKLLELNLLPSHYLDDYKLTNSWKSELKNYISYSKYNYNDVS
                     NSLAVLGEKINPITIKNWVTNKRLIGPIYLKTFEKIFKVIDSKLDHTEVYDATKSIRS
                     LHTKIKKSIDTFVSDNFFSSKSDIIKDEVSAVVLSMLGNTNDYVTPVQIREIHSCDKY
                     LSPHLVNKVLDLYSVY"
     gene            47417..49042
                     /locus_tag="ACOIAS_000757"
     CDS             47417..49042
                     /locus_tag="ACOIAS_000757"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGZ2700477.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVKTISSNKHSFYLTKKDKNHIINNLKLNIYNKDSVLYDRTEYI
                     NELFNIISIEDIESYIISNIDLKNLKIIIKKKKDICDYLNKISNYKKVKSTIEKLETR
                     GKMPKWYLELINKVKNILENMLYNRNLSDEQIFYLNLFLDIQNLFLGIYELKSESVIF
                     ENTLDDNKIIAKMDTYIIIDYINASFEYLGYVLNGSALNSIDYGVPQNRERFVLIGAK
                     KDFLKNKKIETPKPIVGENYVTVRDAIGDLAEYEASKGSMDYCFEKNSNNIEKDFYRK
                     IILDSNKIYNHVCTDTRNIALKRFEYINQGNNFHSLPDELKGTYADPERTQNTIYKRL
                     VYDKPSDTVVNVRKSMWIHPIKNRAVSAREAARLQSFPDSYKFLGTKDSVYQQIGNAV
                     PPLLGRVVAEKILNLFNDEPNEYLRDVFEALDG"
     gene            complement(50042..>50237)
                     /locus_tag="ACOIAS_000758"
     CDS             complement(50042..>50237)
                     /locus_tag="ACOIAS_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HGZ2700478.1"
                     /translation="FGQLENYTDEVFFKENNLVEINYKVQTYTYEIFSVYTADLINRD
                     YLSIHFNNNDEFKYSLNYIT"
BASE COUNT        19856 a         5575 c         7700 g        17106 t
ORIGIN      
        1 ctattaaatc aaattcttac acatataatt attggtatgc ttattaacac ttcagaaact
       61 cttatagttt aaaatttaaa tttattgtta tttattattt aatttgagaa catgatttat
      121 tttttattta aagctaataa gtagagcatg attctacagt ataatttctt aaattttttt
      181 taaaaagtaa tagttcttat ttatagtcaa tattgcctat agaacctctg attcatttaa
      241 tttttaggaa aaaataatac ttacgctata aacacagaat tttagttgat aatataggta
      301 tttaaattta agatgaaata atacttttct caatatattt tattgtaact aagtttaatt
      361 ataagttaca attaaaaaaa cgctacttag gaggaatgta attatgggaa tgataggatg
      421 ttatactaaa ataagtgaag agaatgtttt taaattacag caagcagaag aaaatttaca
      481 agattttgtt tttgaagatg ccaacgaaaa tagtactatc aatattgata aagcatggca
      541 tgccattcac tttactttga caggttgtcc ttttggtgga gatgatgata atatatttag
      601 caaattagta ctaagtggaa acatacttat ggaaattgat ggagaatttc ctgtcatgtt
      661 gattacagca aatgatgtaa aaaaactatc aaaagcaatg aacagtttgg aagaacaagc
      721 atttagaaaa agatttaata ttaatgagat gttagaaaat aatatttatc ctgttatgaa
      781 tgatgagagt gaagatgatt tttttgaata tgtatgggca aatctaattg agcttaaaaa
      841 gtttatacaa gaagcatcta atgatggact agctgtaata ttttttataa tgtgataatt
      901 actaaaaatt gtgaatatta gtgtcatcac atatcttaga tatgaagcct tttgattgta
      961 tagaagaaaa tacatattat attagccttt taaaatatat aagttaaatt taaatctgta
     1021 ggtaaatttg tcttatgtct ttataaatat ttatattagc atatataaat tagttttaaa
     1081 tttttgcaat atatagttgc agatattttt atttatctta aaaggctaaa taccctatcc
     1141 tattatatcc agtaattctt ttggatactt atcacatttt tcattatata taaaaataga
     1201 ctcacaaata aaataaatat atattaaatt ataacttcat aaacttaaaa aatttcagaa
     1261 acaaagatac tttaatttaa ttaaaaacag aaaaattaaa atctttagaa aaaatttaga
     1321 aatttataaa taatatctta agttttagaa tttattatga aaatacagta aaaatttata
     1381 aaaataaaat tctataaaat tgtttctggg ggtgtattta tatatggaaa tgaaaatagc
     1441 agtatccaat cttagtaaaa gatttgggaa aaaacaagct cttgataata taaatcttga
     1501 aatcgacagt ggtatgtttg gtcttttagg aagaaatgga gcaggaaaaa ccacatttat
     1561 gagaatttta gccactttat tatcaaaaac agaaggttca atacatatgt gtggagttcc
     1621 tattgagaat actaaggaaa ttagaaatat ggttggttat ttgccacaag acttttcaat
     1681 gtatcctaac atgacagtat ataaagcaat ggattattta gcagttttat cggatatacc
     1741 actaagaaaa agaagaaaaa ttatagcaaa gttattaagc aaagtaaacc ttactaatta
     1801 tcaaaatgtg aaagtaaaat ctttatctgg aggtatgaat agaaggcttg ggatagcaca
     1861 agcattaatt cataatccca gagttttaat agtagatgag ccgacagcag gtcttgaccc
     1921 agaagaacgt ataagattta gaaatttact atgcgaaatt gcaaatgata gaatcgttat
     1981 tttatctaca catattgttg gagatataga agctacatgt gaaaacgtag ctatattgaa
     2041 tagtggaaaa gtcatataca atggctcaac agaagcatta gcaaaactag ctgaagggaa
     2101 agtatatagt attgaagtag ataaaaaaga tattgaaaat ataaaaagta gatttatagt
     2161 tataggaatg ttgactcatg gtggaaaggc tatacttagg attatatcag atgataaacc
     2221 ttttgagaca gcagtaaatt gtaatccaac tattgaagat ggctatatgc ttataatggg
     2281 aggagataat atatgaatct atttataaaa gaatgtaaaa atatattaaa gagtatgata
     2341 tattatattt ttattgtagt tttagttttt ttctatattt ctcaacttgg aagttcagtt
     2401 tcaaatgatg taagtaaaaa tattcctaat gatggaaatc cattagttgc tccacaaaaa
     2461 gtagagaata ttgaggaata ttactctgta cacaatcaat atccatatgg tcaaaaagaa
     2521 tctatgaaaa cagaaaaagt cattccaaat gcaactataa acctaattag agaatatcaa
     2581 aaaaacacat atacaacgta tccaacaggg gtagttaagt atataaaatt agaccaaaat
     2641 aaaacgaaga ccatagaaaa atatatagaa gaaataactg gtaaaagtat tagcgaaata
     2701 aattcacttt ggaaaactga tgaaagtata aaaatagatt attctaaagt atctcttgaa
     2761 agatttgaag aaataatgca aagagtagac gacattttag gaggagggtc tgcatatgct
     2821 tttgacaagt tacaaagatt tggagtagtt ccagttacct atgatgaagc tatgaagaat
     2881 tacaactata caatacaaaa agataaagtt agtaatgggt atgcaagaat tttttgcgat
     2941 tatataggta tagttttaag tatatttcca gtatttgtgg cagtttttat gtcacttaaa
     3001 gataagcgtt caagaatgag agagattatt tattcaaaac aagtatcttc atgcaaaata
     3061 gttttatcaa gatattttgc acttgtcttt atgatgatat tgcctgtatt aattttaggt
     3121 atgaaagatt tacttccttt gattttcttt gctaaagata aaggtatatc tattgataac
     3181 ttagcattta taaaatatac attatggtgg atactaccaa ctcttatgat agttacagct
     3241 gtaggaatga gtattactat attaacagat acaccagtag caattattgt gcaattaatt
     3301 tggtggcttt acagtataaa ttctacagga ctcaaaggtg attattcaac aatggggcta
     3361 atgataagac ataatagtgt attaaatggg gatattatta tacaaaactt caatcaaatt
     3421 atgattaata gaattgtgat aagtttggga gcattggtgc ttgttttgtt agctatattc
     3481 ttatatgagc aaaaaagaag ggggaatctt gatgtttggg caaagttttc aaaactgttt
     3541 agttctaata aaagaaaact tcaaattaag cactttaact aatttaatat gttcaattat
     3601 attgctatgt atgattcctc taatacaagg agtagctaat ttagacagaa tttcaacagc
     3661 tatatgttta gaaaattatg ttgcaataat agggatagta atgttggtac ctgtattttc
     3721 gctagagcaa tcaaaagaaa ttgatgagat agtatcttca aaagcattat ctcaagttat
     3781 tgtacaatta attaggatac tgatgtcaat tataggtata gtggtattaa tcataggttt
     3841 tgtaaatgtt ttaatatatc ttggatgtaa ttttccagta gttgagtata ttattggaag
     3901 tttcgcatca gctatgttta taggaagttt aggaatgttt ttctcaaatt tatttaataa
     3961 tacaatcata ggttatatga tatctatagg atatttaatt ttaaatatga tgactaaagg
     4021 caagtatgtt ggaaaatttt ttataatgtc tatgagtcaa ggtagttttg atgagaaata
     4081 ttggctgata cttggaagtg ttgtgttgat aattgcatct ctcttaataa aaccagtaaa
     4141 aaggaagatt agataataaa atgagagctt agataaaata aaggtaatgc aaataaaaag
     4201 tacatttatg aaatataaat ctatttttat atacacaaac ctacttacaa tctctttatg
     4261 tatgtagata gatttatata tccgtagtat aaaatatata tgtataccga attttatcaa
     4321 aagctctcaa atttatattt caaagacact tattgctatg acctacacat atgtattttt
     4381 tcattttaat tgttgtatta acaataggac tattgttata aactattgat acctcataac
     4441 aaaaaattct ccatctccta aatgcgtttt ttgtacctta gtctcaacta ttttaaatcc
     4501 ttgagactca taacatttta cagctctcat gttccaggtt cttacattta atacaattgg
     4561 tttagtacta tatttatttc tacattcttg taatcctagg ctaattattt ctttaccaat
     4621 tcccattcca caataattcg gtttcaacca ataccaaaga atacagagct tccttcatct
     4681 aataagttta taaaacctat caattcttta ttgtcattta taaaacttat aaaatttttt
     4741 cttttatctt cttttgctag tgaaaaattc ttctttttaa tttcctccca tggaggtaga
     4801 ttgtatattg aatatttacc ttcatatttc caattagtaa cttcaatagc atgttcttca
     4861 gtaaatttaa ctttatttaa attcatatgt cctcctatta aaatatttaa ggtaattaat
     4921 ggtattttaa agttttagaa aataagtaaa actatttata tttatccata aaatctttaa
     4981 aaattttact aacaattctt gtaccctgta ctttttattg gattcacaga agactataaa
     5041 ctaattttga atcaccttct aattcaaaaa catcacgata tatataattt cttagcttga
     5101 ttaaattctg ttaaatccgt ttcttatagt tgtataatca acatcaatat ctttatgtgt
     5161 attagttaat ttattggttt taggaactta ttattaatat attgtatttg ctccaataat
     5221 ccaagtagta ggtaaattat aggcaaatta tcaaatgtat atctatccat gcttaaactt
     5281 tagagataca ataactacta aattagtgtt ctagtagtta caaaaaagaa tctttaaaaa
     5341 tattaattta acttatattt tttaataaat tttctaaaat ttaataagtt aatttaatta
     5401 tagctctaaa tgatattttc tacagatata attttaaaaa ggtgtgtata agaacaaatt
     5461 cacttaaatt tttaaaatca aataaagttg aatctcttga ctaccttgat tgttttatga
     5521 aattaatata gatgtaaact ttactattaa aaaaatacaa gattttagat aagttaatgc
     5581 taattataaa taataagata agaaatataa tatgacataa gggctaggaa taagataaat
     5641 ataatcaaag aataagatat gagaataact accaagagaa atcattacct taatatgttt
     5701 ataaatgtta gactatgttc ctaaattgaa ttttatatat caagtataat aactttacag
     5761 aatgtggaaa tttttattta aagaatacac ataaatattt ccacactctg attttataat
     5821 aatctaataa aatttatagt gactttataa aatcaataat gatttaataa atatttataa
     5881 aacaactatt tttgcttaga aactttatta ttcatattat cattagtagg cattgctcca
     5941 aaacttagtt tttcatacat tacttgagtt ggagctagcc aaactgtacc actaccagta
     6001 aatgtattta agaaaccttc accatttaaa gcagaaccaa ttaaagattt atctgatttt
     6061 gtaacagaaa aattaacacc agatgttctt gctattgcaa agttaccatc tacctttaat
     6121 tcttcaccat ttttcaattc atactctaca atttcacttt gaggtacaac acattccaga
     6181 acgattacac cagtaccact taattctatt tggaagaagc cttcaccacc aagtaaagca
     6241 gatgagaagt tcttttgtgt aaatgctttt acttctattg tatcagagca acaatagaat
     6301 aatcctttat caacaattat agaatcatta tcaagactca taaataaata atgtctaaaa
     6361 gaaggttcca aataaacttc tccagtacct ttgtatacag gtttcatcat tgattcatta
     6421 gtaagtgctc ctgatattcc ttttttaaaa agacctccaa caccaccaat cttagtttca
     6481 gaagatatat ttcctttata atagtataat gcaccagctt ctgtttttat tgatgaatta
     6541 tttaatttta tcttaatttg tctagctttc aaaccagctt ctctagcaaa atataatccc
     6601 attgccatta atgggctaga agctccctca agtttttcaa attctaatat ctcaaattcg
     6661 cttttgccat ctgatgcatt ttctaaaatt tttacttttt tgcttaaatc catacaaatt
     6721 tctccccact atgataaaat ttatctctaa aatattataa gatataactc tatattacat
     6781 tttttttgaa aaaatcgcta ctttatcatc aaatttttac atatttttaa gaagtttttg
     6841 aaatgtttag atttaagtta agttatttaa ggatgtatag ttatttcaat agacattttg
     6901 agtattattt atcatttgtg ggtattatat tttatgggca tgttaaaatt atttagaaga
     6961 tagaagatag aagatagaag atagaagata gaagatagaa gatagaagat agaagataga
     7021 agatagaaga tagaagatag aagatagaag atttataata tgtaatattt ttgttttgaa
     7081 atttataaat tagtattact ggttagagtt agtttagtat taagtatatt cgaagtagtt
     7141 ttttagctat tttcatacat ttatattact taattatata tagcctaact aacttaatat
     7201 atcagtgaaa tattgactaa aatgcctaaa tatgttatca tacaagttag tcaaataatt
     7261 tggcaacatt aaatatttag taaatttcta acatttatgg aacaaatttc tagcaaagaa
     7321 ggtgagattt atatggctgg taaaaaaata ttagcaacaa acaaaagagc aagacatgaa
     7381 tattttatag aagaaactta tgaatgtgga attgaattaa aaggaacaga agtaaaatca
     7441 ataagacaag gcaaagttaa cctaagtgat ggatttgcat ctgtagataa tagcgaagta
     7501 tttttaaaac aagttcatat aagtccatat gagcaaggaa atatattcaa tgttgattct
     7561 ttaagagtta gaaaactatt acttcataag catgaaataa gaaagcttat tggagcaact
     7621 actataaagg ggtattcact tataccactt agtttgtatc taaaaaacgg taaagttaaa
     7681 gtagagattg ccttagcaaa aggtaaaaaa ctatatgata aacgtcaaga tatggctaag
     7741 aaagatgcac aaagacgtat agaaagagaa atgacaggta agtattaaac tagataaaac
     7801 tgtatttttt agaataaaat tctttattat ctagaacata acattgattt ttattcttaa
     7861 aagtattata ttatatatac attaaaaatt taataaaaat ggtgataaag tttgagaaaa
     7921 gtctactgga ctataaacta ctcaaggggg cgtaaaggtt tcgacgtggg tttggaactt
     7981 gaggctgcat gtcgtgttac tctgggtcac gtaaaaactg gggaacttta aaataaacgc
     8041 agacgataat ttcgcgatag cagcctagtt gctgctcgtc cctcttagtc ctcctgccga
     8101 ctaagactgg acgtcattat gcagggaact actttagggg tgtctcgact tgaagtagat
     8161 taattgggac tggctaagca tagagcctgc cacttggcgc tatgtgaggt gagatattag
     8221 tgaagttggc taagcatgta gatgcagcaa ggaaaagatt tgcggacagg ggttcgactc
     8281 ccctcgtctc caccattgaa atccacgaaa acatattgat agtttagtca atatgttttt
     8341 tttgtgcctt gaaatgatat aattataata taaactaatg taaaatttaa ctaaaaggag
     8401 atagtataag tgagtataga taataatttt aaccatgttg catttctgtg gaatatagca
     8461 gaaagtttaa gaggtacata taaagaagaa gactatagaa aagtaatgtt accactaata
     8521 gtaataagaa gatttgattg tctattagat gattatgata gagaaataat aaaatcagta
     8581 tacgaagaat acgacttctt accagaagaa gaaagagatg agctagtaat agtagactta
     8641 aaagaaaatc acgacataaa cttacaattt tacaatgtaa gtgattttac atggaaaaaa
     8701 ctattagatg atagtgaaaa tataaaatca aactttgaag aatatctaaa cggattctca
     8761 aacaatgtaa aagaaataat aggtaaattt aaatttaaag atgaaataaa tcaattagat
     8821 aaaaaagaca agctatatgc agttcttagc aaaatgtatg aagtagatct acatataaac
     8881 tcagtatcta acaataaaat gggatacata tacgaagaaa tgttaagaag atttacagaa
     8941 aactcagcag caggagagca atacacacca agagaagtta taagactttg tatggaaata
     9001 ctatttatgg gtaaagaaaa cttccttaca gaagaaggaa aagtaatatc tatagcagac
     9061 ttttgttgtg gtactggagg aatgctttca atagctgaag actatgtaga aaaaataaat
     9121 ccaagtgcaa tagtaaatgt atatggacaa gaactacttg atgaatcatt cgcaatatgt
     9181 caggcagata tgctaatgaa agggcaaaat ccagataaca taagacttgg aaatacttta
     9241 acacaagata gatttaacgg agaaaaaata agatttttaa tatcaaatcc gccttttggt
     9301 gtaacttgga aagacgaaga aaagaaagta aaagaagaag cagacttagg ttttgatgga
     9361 agatttggag caggtactcc aagagtaagt gatggttcat tacttttcct acaaaatatg
     9421 ataagtaaaa tgtatgatga tgaagaagga agtagaatag ctataatatt taacggaagt
     9481 ccattattta caggagatgc aggaagtgga gaatcaaaca ttagaaaatg gataatagaa
     9541 aatgatttac tagaaggtat aatagcacta cctactgata tgttctacaa tacaggaata
     9601 gctacatata tttgggtact tactaacaaa aaagaagcta aaagaaaagg taagatacaa
     9661 cttgtaaatg ctagtgaata ttatcaatta atgagaaaaa gcttaggaaa caaaagaaaa
     9721 gaaataagtc ttgaacaaat agaacaaata aaagaaatat atgaaaaatt tgaagaaagt
     9781 gaaaatagta aaatatttga taacgaagat tttggatata gaaaagtaac aatagaaaga
     9841 cctttaaaac taagctttaa agtaaacgaa gaagctatag aaaatgtaaa aaatactact
     9901 caatttataa atcttgcagt ttctaagaaa aaagatatag aagttaaaga aaaagaagaa
     9961 gctgaaggaa aaataaaaca agataagtta ataaaacttt tagaaagctt tgacaatact
    10021 ttagagtata tggacaggga taaatttata aaagatttaa aagctaaatc aaaagagttt
    10081 gatatacaac tttcagcagg acttataaag gctatagtta atactattgg tgtaagaaat
    10141 gaagatgcag aagtttgtaa agatagcaaa ggtaatatag aaagtgatag ttcattaaaa
    10201 gatactgaaa gtataggtct taaagatgat gtatatgagt attttgaaaa ggaagtaaag
    10261 cctcatgtta atgatgctta tatagatgaa agtagtataa ataatatagg atatgagata
    10321 ccatttacta gacattttta taagtatgaa gagttaagaa gttttgctga gattatgaag
    10381 gaagtagaaa gtttagaaag tgagatagca ctaaagataa ggaaggtgct agggtaatgg
    10441 gatacagata tagaagtgat gatgagttaa aggatagtgg agttgagtgg ttagggaaga
    10501 ttcctaagga ttgggaagtc agacaattaa aaagggtact atcagataga aaagaaaata
    10561 atgaacctat aaaaacaagt aatatactgt cattgacaat agaaaaggga gttattccat
    10621 attctgaaaa aggtactggt ggtaataaag ctaaagaaga tttaacacaa tacaaattag
    10681 tatatccaaa tgatattgta ttaaatagta tgaatgtaat tgttggtgca gtaggactat
    10741 caaaatattt tgggtgtgcg agtcctgcat attatatgtt atataaaata aatgctaacg
    10801 atgatataag attttataat tatattttta aatctaaatt attccaggat agtttaaaag
    10861 gattaggtaa tggaataatg atgaaacaat cagaaacttc aggtaagtta aatacgatca
    10921 gaatgagaat tccaatggaa aaactaagtt cagtaaagat accaattgct tttaacttag
    10981 atcaagaaaa aatagcaaac tttcttgatg aaaagacttc acagtttgat tcaattatat
    11041 ctaagaaaga aaagttaata gaaagattag aagaagcaaa gaaaagcctt ataagtgagg
    11101 ttgttactgg taaggttaag gttgttaaga ctgatgatgg gtatgagctt ataaagagaa
    11161 gtagtgaaga gatgaaggat agtggagttg agtggttagg tgagatacct aaggaatggg
    11221 atataaaaag acttagattt ttaggaaaac tacaaaacgg tataagtaaa agtagtgatt
    11281 cattcggtta tggttatcca tttgttagtt atagtaatgt atatagaaat atagaattac
    11341 cacatatagt agaaggtcta gtaaattcaa ctgaagaaga acgtaaaatt tattcagtag
    11401 aagaaggaga tgtattcttt acaagaactt cagaaacagt agaagaaatt ggttttgctt
    11461 ctacgtgtat gaaaactatt gataattcta cttttgcagg atttttaata aggtttagac
    11521 cgaatgataa aagactatac aaaggatttt ctaaatatta ctttagatgt gatttaaata
    11581 gaaaatattt tgtaaaagag atgaatttag taactagagc atctttatct caaaatcttt
    11641 taaataatct ttctgttgta ttgcctaagt atgaagaaca agttgagata tataatgcac
    11701 ttgaattaaa agttagctac attaatttat cgattaataa gttaaagtac caaatagaaa
    11761 aactaaaaga agctaaacaa agtttaataa gtgaagcagt aacaggaaaa atagagatac
    11821 tagattaact tctagtatct taatatagat atagattata catacaaagt taaataaaag
    11881 ggagaattaa tggataaaaa tatatttcaa attaatataa atacagatgt actgataaga
    11941 ttaaggaatc aaataaatga agaatattat atatcttata gaaaagaatt ttatgataaa
    12001 aataataaaa aacatcgcgc ttgggataaa atatgtgcaa taatggatag gcttgacgat
    12061 actgtagatt atttaaataa actcaaatta aatacaggta aatatagacg aagtgcattt
    12121 gacttctttg aatttatgaa tcaggcttcc gtagtaatgg attgtataac acatcttgca
    12181 aaaatatttg aagtaccata tgaagaagtt gaaacttcaa ctgaaatttt taatgagctt
    12241 ggaaaagagg gtaaaggtac agataaaaaa tactttgaat atctacgttc attgtgttca
    12301 gtccatccaa tggaaactag tagacacaaa atataccaag aaaatagttt tgaatgtagt
    12361 ccttatgtag tttggaatga taaaagtata tggactaatg atgctagcga tttatatgca
    12421 gtggtataca caagtaaaga taatgatagt ttcaaaagaa ttcatatata tatgaatcaa
    12481 atatttgagt atgtaaaaag aagagtaaat tttattgaaa atataataga acatatagaa
    12541 aagtatcaac aagaaataat tgaaattttt aggaatactc caataaagaa agaagaagat
    12601 tttgaaagtt atatagatta tttaagaaat ctaaaaaaag agatagaaat aagatatggt
    12661 aatgattctt ttcatgaatt aaactacctt atagaattat ttgatttgcc attatcaaat
    12721 cctgataatg aagagaagat gaacttatat aaaaatgctt taaaatatgc tatcaaattt
    12781 gagcataata gtattcaaaa tatgacaaat gaaggttata agaataatgg aatattatat
    12841 ccacaagtga atataacaac tacattacta gaagagctaa tttcattaaa tagtggtagt
    12901 gaggagagac gtaagtacag atatgcaata gaaaaaacat catatctaag ctatgatagt
    12961 tgttggagtg atagagaatg ggcatatgat caaatagatg atatgatgcc ttttttagaa
    13021 agatacgtaa catttgataa agctcaaagt gattttgagc attatgtatt ggtaaaacta
    13081 gcactttatt tagagtgctt agaaaatgaa tgtttaataa ataagaacgt tcctaatgat
    13141 aaaaagtttc gtattaaata attatagcta ataaaatagg gagagggagg atattatgtt
    13201 accaaaagaa aaacacctag aagaatctat tgaaaatcat ttagtttcaa ttggatatga
    13261 acaaggtcat tcacaagaat ttgaccttga aaattgctta tttgtagatg atttatttag
    13321 attcttagaa aatactcaaa aagaattact agatgagttt aaatacaaca ggggaagtaa
    13381 ttacaaaaaa gcttttatag atttattaaa ttcaaacatc aaaagaagag gtcttataag
    13441 tgtacttaga aatggtgtag aagatataat gatgaacgga aaatttcaat tattctacaa
    13501 caaaccaaat ctaactacta ataaaacagc gtatgaaaat tataaacaaa atatattcaa
    13561 aataacaaga caattaaaat atagctcaaa gcacaacaat actttagata tagtattatc
    13621 tttaaatgga tttcccgtta tagtaatgga acttaaaaat cagtttacta atcaaaatgt
    13681 gtataatgct ataaagcaat tcaaaaaaga tagaaaccca aaagaaactt tatttggatt
    13741 taatcaaaga gtgttagtat attttgcagt agatactgat gaagtttata tgacaacaga
    13801 gctaaaagga gataaaacat tcttcttacc gtttaataaa ggtaacaata gaggtaaggg
    13861 aaatcctgta gtagaaggaa aagaaaaaac ttcttatctt tgggaagaaa tacttactaa
    13921 agatagttta cttgatatta taaaaagatt ttactttatt caagaagatg ataaaggtaa
    13981 tcaaagagca atatttccaa gatttcatca attagatgca gtaaggaaga tagtagaaga
    14041 tttaaagata aataaagtag gtaaaaatta tcttatccaa catagtgctg gtagtggtaa
    14101 gactaacact atagcatgga cttcacatag gttatcaagt cttcatgatg aagaaaataa
    14161 tgctatattt gactctataa ttgtagttac agacagaaag gtacttgata aacaattaca
    14221 aaatgctata tatcaattag agcacaaaaa cggacttgtt gttaagatag atgaagataa
    14281 aaattcatca gacttagcag atgctattgt aaatagagca aagattatta taactactat
    14341 tcaaaaattc cagtatgcac tacctaagat agaacaactt gataaaaggc gttatgcagt
    14401 aattatagat gaagcacatt cttcaacatc tggtgaaaat atgaatgcat taaaagaaac
    14461 tttagcagga aagactttag aagaagctaa ggtaattgat gcggaaaatg aaaagaatga
    14521 aaagtctagt atagataaga tgaatgagct tatagagaaa agaacaaatt taggaaatat
    14581 aagtttcttt gcttttacag ctactcctaa gaataagact atgcaagtat ttgggaggat
    14641 aggtgaagat ggactacctc atgaatttca tttatattct atgaaacaag cgatagagga
    14701 aggatttata ttagatgtac taaaaaactt tatgcctata agtgtttatt acaaagtagg
    14761 aaagaaaatt attgataacc ctgagtttga taagggagag gctaagaggg ctataaataa
    14821 gtttgttagt ttaaatgagc acaatataag gcaaaaggtt gaaactatag tagatgattt
    14881 tgtagataat aggtctatgt ggttagaagg taaagctaaa ggtatgatag ttactgcaag
    14941 tagattacat gctgttagat ataaactagc tatagaccaa tatataaaag aaaaaggata
    15001 tgatatgaaa gcacttgttg ctttttctgg tactgtaaaa gatggtgatg atgagtatac
    15061 agaggtaggt atgaataagg aagtagcacc agatattact gattcaaatt tacctaatat
    15121 atttgataaa aatgaattta agctattaat agttgcagaa aagtatcaaa ctggatttga
    15181 ccaaccaaaa ctttgtgcta tgtatgtgga taaaaaacta gatggtgtta aaactgttca
    15241 gactttatca aggcttaata gaacttatcc taataaacaa acatttatat tagatttcca
    15301 aaatagtgtg gaagacatac aagatgcata taagccatat tttgaaatga ctaatattga
    15361 taaggtaaca gatccaaatg tagtaaatga cctttggtat cagttacatg aatatgcagt
    15421 atatactgat gaggaagtaa atgattttac agtattattc tataaagaga aaagaacttc
    15481 ttctcaagat gctaagatga ataatttaat tgataaggca gttgaaaggt attgttattt
    15541 agaagaagat gatgaaaaaa aatctgatga gtttaagaaa aaatgtcaaa agtatattac
    15601 tttttataat tttttaattc agatttatcc tataaagaac ttaaatcttt taagattgca
    15661 agtttattta gtagcacttt taaagaagtt acctaaaaag agtaaacaaa gaatagacct
    15721 tgatgatatg ttaagccttg attattataa attgcaaaag cttggattag atgataaaaa
    15781 aggaacggat atatccttat ataatggaga aggtgagtta gttggtatat ctgaaactgc
    15841 gacttctcgt gtaagtgaag ctgatgaaga gtatttagag gatataatta aaaaaataaa
    15901 tgatgttttt ggtgtaggac ttacggaaga tgatagaatt atgataaata cttatgaaga
    15961 gatgtttaag aatgacaagg atatcatgga tatagctaaa gctaatagtt atgaagattt
    16021 agttagcact tttggtaaga agtattttaa gagaggtata gttaaaacca aaaatagaaa
    16081 tgatagatta gttaaggaga tattaactaa taataattta caaaattata tgattcatta
    16141 tttagctgaa aagatatatg aagaggcaaa tagataagat acctatagag agctaattaa
    16201 ctctccatag gtactttatt atgagctatt gtatattatt catcttctaa aggtgtagca
    16261 attcctttta attctaaaaa ctttaagtct aaatcaagaa taagctctat agtatctcta
    16321 tctatagaaa caccttcttt tttacactca gtaagtataa aatcaacttc ttcttcaaac
    16381 tctataaatg gtttatcgtt attcatattt ttgctccttt gattaaatat aatgtattta
    16441 ctttaaatta tatcaaagaa gagaaaaaaa taacattatt tgtaaaaaac tatatatata
    16501 taatatatag ttttaccata tatagataaa ctgtttttaa ttaaattata aatatatatt
    16561 atattaatga atctatatta aatagatagt tgagtaagta gtaataccaa aaatattaaa
    16621 aagagatgtt ggtagaaata cgaacatctc ttttttagtg caaaaaatta aaaggaggaa
    16681 gttaatatga ttaaattatg gaagaaagaa gatttaaatt tattattaga atatccaaag
    16741 gaagtagttg aaaatgttga taacgtcatt aatattcttg atgaaaacta tggatgtaac
    16801 agaaaattaa cagatgatgg tggatatgtg tgtataattg aggatattaa agaagtagaa
    16861 aacttaaaat caaatatatt aaaggggtta gtagaagagt ttagtgatgt aatatatgaa
    16921 gatgaagtta atacatataa ttcaacacta tacttgttat ctagtgatta tagtgttact
    16981 gtaatttcta aaaatgaaga aactgagtac ttacttaaat aagcatatac attgataagg
    17041 tgtatatgct tattttaatt ttaataaata aaggagaaaa gcagaatgaa taaaaagaga
    17101 actcctgcca agagagttga tatagtttta cttaaattag taaaagaacg tagtgttcta
    17161 tatgaaacta gaaaaataag taatctttat gatgcttata gattagcaaa gaatttttta
    17221 taaatagtgc tagagaaaag tttgtagtgg tatgtttaga tacaaagaat tggccagtag
    17281 catagaggtt gtaagtatag gcactgtaaa tttatctatg gtatatccaa gagaaatatt
    17341 taaagtagct atattaacca attcatctga aatgatatgc tttcacaatc acccaatggg
    17401 taatactgac tttagcaagg aagatagtta taactaatat gctacagaag cgtggagaaa
    17461 tattatgcat tgaattagta gatacatata gttattgtag atgaggataa atactttagc
    17521 tttaaagaaa actgtaggat ataagagtct tagcttaaat aaattaggct aaggcttttt
    17581 ctatgttcca aaacaattaa aaataaatca aatttaggtt aagcccggaa agcccttgaa
    17641 aaaaataata catataaatt taaaactaaa taggagttgt aacatgttta aaaaagaaaa
    17701 taggtatatg acaaaagaga tagctgaaaa tctaccaatt gaaatagcta tactactatg
    17761 gaatttaata gatgacttaa ctacagaaaa agattatcta caactatttg aaataaatcc
    17821 tataggatta ggagtagttg agatagttca taagcaagaa gtacctgaat atgaagctag
    17881 gatatatata cagaatgata aaattaaaga taaattaaaa atatatgtta tagataatgt
    17941 tgaatataga acaatgatgt tttcaaatga atattagtta atgattaaag gagagagagt
    18001 aatatgataa ataataaatc tgaaataatt aataagttat ttttcaatga attaaaagag
    18061 ttaatagata aatacaacaa tatagatgat aaaactgtag atgtaataga aaagatattt
    18121 acatgtatag aagatgaaga aataaagaat tacctaataa gagataattg taagctacaa
    18181 gagctagtaa atacatacaa taaacttgaa gatttaaaca tagatgaagc tatattcttt
    18241 gcttggtaca acttaactat aaatacaata tctatagata aagcaagtga gtactacgat
    18301 gatttaacta caagtaacta tatagaagta gaaaatcata taatatatac aaatgaaaga
    18361 gatttaagga agtatgctga agatgaatta gaaactatgt taagcatgga acatcatata
    18421 gatagattac tacataaaga catgttaata gatatgtggt taaacaatac ttcaaaagaa
    18481 gaagtaatag aagaaatatt aatgagtgat gatatagaag atatattgaa tttatctcca
    18541 gaatatgcat ttacattagc taatggaata gaatatagat actcagaaaa agaagaataa
    18601 aataagtata tgtactgaaa aatacgggct ttaagggctt atacattaaa gtacatattt
    18661 gaaaatacca tagattttac aagagaaaat cagaatgaaa ctttttcaaa tactagttat
    18721 tattaattag tgctagaaaa ggaggtggag tgatgactaa caacaataat aattgtgatt
    18781 ataagggcat atatgcagat atggttgagg ttttgggtga agaaatagtc attaaattac
    18841 ataagtatta tcgtggacaa caaataacat ttccaatgaa actatattct attgaatatg
    18901 tggaacgata tatagtgaag aattatagaa caaaaacaat aaaagaaatg tgtagagagt
    18961 taggatatac agagggatgg ataaagcaat taattaataa aaatagatta aattcaaagt
    19021 aattagagaa ggagattgac agtagatatg atattaacag tattagcaat acttatgggg
    19081 acaggtatat tttttctgat taattcaatg ctagatataa cgtattttgg atgtggccca
    19141 ataggatttt tatggtttgg atgttttatt gtgtcatatg caatattaac atcattaggt
    19201 ggtatagttt taggattgtt aaagtggata gtaataggat tgattatact atttgtaata
    19261 ggattaatag cgaataagat aaatgattaa attactgtag attattttga taaaataatt
    19321 atttaatata ttgaggcatt acatgataga agaataaata taaatgactt aaaatcttta
    19381 acttaactaa acaaaaaata tattatttat tacaaaaaat gacaaaaaaa ttaaatatta
    19441 tattgtatta tgacaatata aaacattgga gtttataaat taggaggaat attttatgca
    19501 agattttgat tttataagtg agacaataaa gaaatttcaa tccgatttag aaaacatgga
    19561 taaggtaaat atacttgttg taggtaaaac aggagtaggt aaaagtacat taataaataa
    19621 tatatttaga gaagaattag cagaaactgg aattggtaga cctgtaacac aacacttgaa
    19681 aaaaataagc aaagaaggat taccaataaa tttatacgat acaaagggat tagagttaga
    19741 agaagatgtg caagagacta taaaaaaaga gatattagat gaaatagaaa attgtaatag
    19801 aaattcatta gaaagaaatg ataaaagtga gttaatgcat gtatgttggt attgtataaa
    19861 tacatcagta gctagatgtg aagaaactga tataaaatgg ataaatgagt taagtgaaaa
    19921 gataccagta atagttgtat tgacacaagg attcccaaga aaaaaagcaa gagaattaaa
    19981 atctagtatt gatgatttaa atttaaactg taagaatgta attccagttt tggcagagca
    20041 actagtagaa gaagaggatg agatagttat aaagtcatat ggtttagata aattagtaga
    20101 agttacatat caagtaatac caaaagaggt caagaaagct tttgttaatg tacaaaaggt
    20161 agatataaaa aaaaaggttg aagagtcgag aaaatgggtt ttaggatatg taggaggatc
    20221 aggagcaata ggattttcac caataccatt ttcagatgct ccattattag caaccgcaca
    20281 agttgctatg atatctcata taacagtaat atttgggtta caagttgata aagctttact
    20341 aacggcaatt gtaagttcta taggaggggt tacatcagct actttactag gaaaaacatt
    20401 ggtatcgaat atacttaagt ttataccagg agctggaact atagcaggtg gagcgataag
    20461 tggttctgta gcggcattaa caactacagc attaggaatg gcatatataa gtgtattaga
    20521 atatatacta aatgagaata tgaaaggtaa tgaggttaat aatactgaaa tagctgaaaa
    20581 aatgaaagat gcatacaaag atcaattagg aatgttatct aagaaataat taattcaaga
    20641 taaagtataa gattaattat agggaaaaag taaaatttaa aggtatattt ggtaaaacat
    20701 aaacaaaaat gataatggcg tcaatattat ttgttgtcat atgtagtagc ttagtatata
    20761 tattattgag tgataggttt aatcataagt taatttactt agagaaagat atattagatt
    20821 taagaaataa aaatttgtaa ctagctaata aaggatcagt atagaaacag ataaacaaga
    20881 ggataatgta gatattaaat ctaaaaacac taataatgaa gaatataaaa cttcaaaact
    20941 atttaggcac tagatagagt atttgctata atgaataagc taatagagaa attaaaacgt
    21001 ttatagtaag ataactaatt caaatacatt tagtggactt caaggaacat ttgatacact
    21061 atcaaactat ggtagctaat tgatagacca atcatctaaa ttgaatttat caaatgttgg
    21121 atggttagaa atatctgata taattacaag tataggaaag gactattaga ttctgaaaat
    21181 atgtcatttg aagcattaaa tataatggga aaaataggaa aatttataaa ttatattgaa
    21241 attaattata ttaactaatt aaatattgat acataatgcc ttgctacagt taaatgtggc
    21301 aaggcattat ttttgtaaag aaaggaagaa ttaacatggc gaagcaaagt aaagaggaac
    21361 tatttgaaaa agaaactaaa tattgcaaga taataagaag tgtaacagta gaagatggag
    21421 atgaaagatt tgcaatagag aaaatatata ttaagtcatt acaaagatac gaggttagaa
    21481 tatgcctgta tcgtgactgc cgagatagaa ttaataaact tattccaagg cctgtagatt
    21541 taactatgga taagtttgta gcattaatac tggtaggatt aaaggctggt gtactagatg
    21601 atgatttcaa gcaagaacta attaaaggtt tagcttagga ggtagaatat gacaatagaa
    21661 gagtttacta gtggagtaat gtttgggatt tcaattttta gtgcagtatt aaagcaagta
    21721 ttagaaaata aaaaatagta tacaggaggt atgagtatga ttggatttat aataggaagt
    21781 ataataggaa ttggactatg tattagtata gagatactga tatcgaaaaa tgccataata
    21841 tactaagtta tatttaaatt attaatagat tcatatataa tttatttaag cccttaaagc
    21901 ccagataagt tatattaaag taaagtttgt atttatgtaa attaaataat atatattatt
    21961 ataaatttga aaggacttta taaatatgaa aggcaatata aacaaaaaag atatagattt
    22021 aagtaaaata acatttgata agtttttaga agatgaagga ttacaaaata gagagagaga
    22081 ttacttatat gtagatgatt atgacaatac tttagatact caagaattta aattacatat
    22141 aattgaagag aatactgata acacatatat agataaagat aaatactggt atttagattt
    22201 agatgatagt ttatttgaat acgaagaaga tgaagatact attgctaaaa cattatatag
    22261 tctaatagat attagaatta aatttttgaa acatgttgag tatagaaaat atataaatct
    22321 aggagtaata gacgaagaat attttgatga gattaaatca tttttgaaat tttatatagt
    22381 agcaaaaaca aagaaaaatg agctaggtaa ttcaaattta aagcagatgg ctatagatat
    22441 ttttgataaa gaaaggtttg aagacgacat atcagatgat gatatggaaa atataagtga
    22501 atggatagga gaagatcaaa actttaaaat gttttatgat aaagtttgta gggatctctc
    22561 aaattctaca caggataaag aaatattaaa attagcagga atcaggaata ataaagtaaa
    22621 acattacaaa ggaagaaaaa ttagtagaaa atgtaaatta gaacttaaat cattgactaa
    22681 aatgtgggag tatgaaacta aactgactca tgatgcaaat aaggcacata agaaaataaa
    22741 atctaatatg cttgaaaaaa tgacagataa gaattataaa aatatatatg aatatttaaa
    22801 taatgagatt ataaatagta atgaagaata taaaaatatt tatttttata gcttagaatt
    22861 aaagttagcg aatgaattaa gaaagtgtac tcttaaaaac ttaaataaaa ttaaagacat
    22921 agaaaatcaa gtaaaattta tttcttatac tattgcttat atacacatag ttccagtttt
    22981 aacgataaga gagaaactaa gcgataagtt atttaatata ttgtcaacta atgaaggtaa
    23041 ggatatagaa aaatttacta aagaagttag tcttataatg agaattataa atatagaatg
    23101 tttggagtta ttaaatatag ttagtaagaa caactattat aaaaatttta gtgaaaagga
    23161 attagaggag tttaaatcta tttataatat aaataatgga agaaagtcat ttgaaaataa
    23221 atataaaata aaagtagatt ataatataga tgatgtaaga aagtatggag aggttataaa
    23281 agcaataaat atagaaagta agaagaaaat tgtagataga attaagaaga agagaaaatc
    23341 taattagatt ttctctattt tttttgaaaa aaataaaaaa tatttggtga aaacgtttat
    23401 ctttaggtga tatgagtagt cattatttat tatataatca tatttgtagg aacgagctaa
    23461 ttaataaaat ctaagaacaa tagcttaatg atgaaaaagt acggcctttc gggctttgac
    23521 aacaggaggt atattaatga caaactttaa aagtgtaaag gtagaattaa ttctacttat
    23581 tgaatgtgaa gaaggaaatc gtaatgaatt agaaggtatg attgatgaag gtgtattaaa
    23641 tgaaaaagaa tatagtttaa ctatattagg tagtacagag tatgaagata acgcaagggc
    23701 tatttacata cttatgaata caggaggatc atacgaaaaa aacttacaac gattaagtag
    23761 attacacttg aaaattgaaa acttacttaa agatacaaat gttaaatata gaggtatatc
    23821 tttagtaccg aatattgtta aatgggataa ataggaggaa tacaaatgag caaggcaata
    23881 aacagagaag aattaattaa attatatgag gaaataggat taaatgtttt agataataac
    23941 accgttatga attcatctaa taaaaagata tgtaattttt tagtaaatat aatagaacaa
    24001 agggttatag atggaaaaat gtatttatgt ataaatgcta tggggattaa tggagagcta
    24061 ttatcagagg taatattaga ttcgaatacc ataaatagaa ctacatgggt gattgaaaat
    24121 tggggtttta aatattctat agataaaaaa tatattaatg actttattga attgatacaa
    24181 cacttatgta tgaatataaa aactaagtat atatatgacc atacaggatg gatatatcaa
    24241 gacaataagt ggatatatct acatggagga ggagttatag gaggttcaga tgttcaagtt
    24301 gagttagatg aatcaataga taactatata ctaccagata aaattagtga cataaataaa
    24361 gcatgtgaaa tgagtttaaa gctagtagac ttaatagaag atgataaagg tattatattc
    24421 tcttcattag tttacctatc tccacttatt gaaattatca gtagaaaaat taaaccaccg
    24481 gaaaatatag tatggacata tggaaaaaca ggatcaagaa aaacagcagt atcaaaagtt
    24541 gtattaagtc attttggtga ttttaataaa aaaattccag ctacatttaa tgatacgatt
    24601 acagctattg aattaaaagc taataagtta aaggatagct tatacttatg tgatgacttt
    24661 gcacctaaac aagactttag ggataaaaaa attcaagact caaaggcaga atccattata
    24721 agaatgtatg gagatagagt agggaaagga cgctcaagta ataagttaga tataaaggat
    24781 gctaagagac ctagaggaat gatattaata acaggtgaag atgtgatatg tggagaatca
    24841 tctaatgcta gattattatc aatagaatta aatagaagtt cagtagattt aaatatacta
    24901 actgaattac agaataatag tcatttgctt ggagaagcaa tgagaggata tatagaatgg
    24961 ttattatctg aaatgggaaa tattgatgaa ttatctaata tattattata taactttaaa
    25021 tcttacaggg atgaaattag agaacaatat aaagatgata tacatggaag aactattgag
    25081 tctagtgcat ggatattagt aggttttagt tgtatgttaa attatattag agataaaggt
    25141 gttatagata agtatgaaga tgatgagtac atggatagag cgattaaagt tgtagaaaga
    25201 ttaatagaag agcagataaa attaatatcg agaaactcac ctatagatat atttttaaat
    25261 acattaaagg aatcaataaa ttcaaagtca ataagaatag caacattaaa tgaccagaac
    25321 caagttatag aagataaatt agaatatata tgtggatata aggataagga atattattac
    25381 ttttacacag ataaagtata ttcttttata gaatttgaaa ataaaaaaag aggtatacat
    25441 ttgggaataa gtcaaagaca actagtgaaa cagttaagag atagtggaat tgtgaaagtt
    25501 gattctaaca atgattcttc taaaaaaact attcatacaa aagatgctag cgattcaaca
    25561 ggatatacaa caataagacc aagaatgctt cagatagaaa gaagtataat tgagaacttt
    25621 caaatataac ttataatcaa gagtgcttat gtactcttga ttatattcta tgataaatgg
    25681 aggatataaa tatgacagaa acttataaaa tcagcatagg aagaaaagat ttatattcta
    25741 aagactataa ggtagttgaa agaactgact atgaaaagag acacacagtt aatataagta
    25801 gaagcttagt agacatactt tctatacttg taaaagaaga tagatagatt aaaatatggt
    25861 tacacactta gtgttagtgt gtagccttta atatagatta aaattagtat atttaaactt
    25921 tggaggaaat aatatgacag gaaagaaagc agtagtatat gcacgcttta gttcagataa
    25981 tcaaagggac gaatcaatag atgctcaatt aagagcaata aatgaatatg cagataagaa
    26041 taatataaaa atagttaatc aatttataga cagagcaaaa tcagcaacat cagataaaag
    26101 gccagagttt caaaatatga ttaaattctg tgaagcagat acaacaggaa tatctatggt
    26161 aatagttcat aagctagata gattttctag agataagtat gattcagcta tgtacaaaca
    26221 gaaattaaaa gtaaaaggaa taagagttgt aagtgtatta gagaacttag ataattcgcc
    26281 tgaatcccta atattagaat cagttataga gggaatggca caatattaca gtgctaattt
    26341 agcgagggaa gttgctaaag gtcagaaaga aaatgccctt aaagcccttc ataatggagg
    26401 cgatgcacca ttaggttatg atgtagcaca tgataaaaca tatacagtta atgaagaaga
    26461 agctcaagca gtaaagatta tatttgatag atatattaaa ggatattcat atagtaagat
    26521 aatagatgaa ctaaatatct taggatataa gactaaaaga ggtaataaat ttggtaagaa
    26581 tagcttacat ggaatattaa ataatgagaa atatacagga gtatatgtat ttaataagac
    26641 tcagagaaaa ggtgtagatg gaaaaagaaa tggacataag caaaagagtg aagaggaaat
    26701 aataaaaata gaaggtggta tgccacaaat aatagacaaa ggagtattta aacaagcaca
    26761 agatatgatt caaaaaagaa agaaaactcc aggtgcacat aaagctacta ctacatatct
    26821 attaacagga ctaattaaat gtggtgaatg tggacatgca tttcagggga ataagagaaa
    26881 agattcttat ggaaatgaat atgtttcata tagatgtgga tgtagaaagc aaaagagaga
    26941 atgtaataat agagagataa aaagagacta tttagaagag tttatattac aagaacttga
    27001 aaagaacata ttaaatgatg aagctatacc gattttaagt aaagcattga atgagaagct
    27061 gaatgataag aatgaaagta atgcagagct acttaaaaat ctagagcaga aactagataa
    27121 ggtaaataaa gagataagta atatactaaa tgctataatg aatggtatag taaatagcat
    27181 gttaaaagat aagcttgatg agctagaaca agtaaagttc aacttagatt taaaaatgaa
    27241 tgaactaaag atagaaaata aagttgtaga tggtgtaggt gtaacagaag atcaaataag
    27301 aggtatgttt tcaagattta aggaatttgt actagagaga aatatacctg aatgtaagaa
    27361 gtttatagga gactatataa aggaagtaat cgtatataaa gaccacattg aagtcatatt
    27421 caatgtggtc ttttgttttg ttgacaatga tataactcat gatttacata ttatcacaga
    27481 aaggggtaaa attacatagt aaatatatat tttataaaag taaatatcag atattattta
    27541 gataaaaatt atttttgaaa atattagttt taaaaaatat atttatataa ttataaaata
    27601 aaatggtata ataaaacttt aaatatgata aagaggtgag actattggca gatatattta
    27661 cagtactaaa ttatcaaggt agtaaaaaga gattattaga ttttatatat gaaaatacac
    27721 aagaatatat tgattctgat aaagcttttt tagatatatg ctcaggaacg agtgcagtag
    27781 cttataatat gaaaagacac tttacggttt atacaaatga tagtgaaaat tatgcatatc
    27841 agatatctaa ttcaattgtt aagaataatg aaagatataa ctttagtcaa tcagatgaaa
    27901 tgatcataag agaaaaatat aatattaatt tacaaagatt aaataatatt tttaacgata
    27961 tggtaaatca ggaagatatg ttattagaac aagaagatgc agacggttta atagagctat
    28021 attcaaattg cccatgtata tggaaaaata atgttgatat tgaaatagaa ggcaatacaa
    28081 tacagagtat atatgattta agaaaatgta aacgagacat accgaatgtg ttattcacat
    28141 tgtattattc aagtagttac ttcggcataa agcagtctat ggagatagat tctattagat
    28201 atgctattga atttatagaa agtgaaataa caaagagcat tctttttaca agtttatttt
    28261 tcgcaatgaa agagtgtgtt tttgctaaag atggtcacat ggcacagcca ttaaatatgg
    28321 aaacaggcaa gaaaagaatg ttaagattaa aagcaagaag tatatatgac atatttatag
    28381 ataaattaaa ggaatttaat actgaggagt ttgtattatc acaaaaggag aataagtgct
    28441 tcaataaaga ctttagaaca ttattagaaa cagaagaaag tataaaagat gaagttggtt
    28501 ttatatatat agaccctccg tatacagata tgcaatactc aagatattat catattctta
    28561 atacagttgt tttgtatgat tacccagaaa tatcattgag tagaggaact atatcaaaag
    28621 ggatatatag agaaggtaga tatcaatcac ctcttagtca aagaaaacat gcattaaatg
    28681 agattgattt cttatttagt ttctgtaagg aaaataaaat aaatgtagca ttaagttatg
    28741 catatccaat agatacagaa aaacaagcaa ctaatagata tacggctagt attgatgatt
    28801 tgataaatgt agccaaaaaa cattttggag ataatgttaa ggtagtacat gaagaatata
    28861 gtcatgcaaa taatagaaat agtgataaaa agaatgtact tgaatattta ataatgggta
    28921 tacacaataa ggagtaaata tggataaaat taataaatta aaagagagga tagctaatat
    28981 agaagcgacg aataaaaatg atatatattc ttctaactta tattggtcac aaaaaccatt
    29041 caatatatgt gatgaaatta ttgaacattt aagtgaagat ggagatataa tatttgatcc
    29101 gtttatggga tctggggtta cagttataga ggctttgaaa aaagagtata aaagaaatgc
    29161 tattgggtgt gatataaatg atgcaccaat atttatagta aaaacattat tagaaaaata
    29221 taatgtgcct aaaatagaga aagatattaa agagctaaat aataaattaa gtaaattatc
    29281 aacttactat tcaacaaaat gtcgtgaatg tggagaagta gttactatat cgaaaactat
    29341 attcgataag gagaatagga atgcaaagtg tgagctaaag tctatacatt ataaatgtaa
    29401 ggaatgtaaa aatgacttta aaggtgaacc aaatgaaact gaaatatcta aattctttat
    29461 tgatgaagaa ttaaagaata tagatgattt tcaattcata gccaattcaa ggcttgcagt
    29521 tggcgaaggt gatacagtaa gaactatttt ttcaaataga aatttaatag tattagataa
    29581 aatattagga catatagatg aatataaaaa tagtgaatct tatgagccta tgaagtatat
    29641 tttattatct attttacatt taagtaagat tacagataca cattctaatt ctcagtggcc
    29701 tttatggatt cctaaaaaag actgtgttga aaaaaatgta gtagaattat tcataaagaa
    29761 tagtaagaaa accattaagg cattaaaact agcatctaaa tatttatgtg aaaatagaga
    29821 aaatgtagaa ctaatcaaaa atctagatag agggaaattt aaattgataa aagatggagt
    29881 tcaaaatata gaagatgatg ttatacctaa tgaatttgtg gacttgataa taacagatcc
    29941 accatatatg ggacaagttt tatattcaga gtatatgcag ttatataaac cttttatgga
    30001 tttggaattc gaattagaaa atgaaatagt tatatcaagt gccccagcta gagataaagg
    30061 gaaagtatta tattttgata tgatgaattc tgcatttgaa aatatatcta gagtattaaa
    30121 ggtaggtaaa tatatgtgta tgtacttcca tgatagtaat ctagatgtat gggataatat
    30181 aatagatata atgaccaata ataatttgaa atacatggga caaattcatg taagtaagaa
    30241 taaaaacact cttaaaaaca tacttagtcc taagaagtcg ttaaatggag attgtataat
    30301 tttctttaag aaagatttaa atataaacta tgatataaat gaagtagata atatagaaga
    30361 taagataaat aatattgcac aagctattat agatataaaa ggatacgcat caactccaga
    30421 gttatatgat aatggagcac tagagtatat aatatcaaac ggaaaactaa aagagatttc
    30481 taaaaagtat aaggatttaa caagtatatt tgagcaaaga tttaattggg ataaggaaag
    30541 aggggtatgg actgtataat aaataaaaag catctataga taattataga tgctttttat
    30601 ttatatttta tagtaaacta aatatttaat aatttctaat ttttatacaa tatgttaaag
    30661 gattggctat ccaaagatat tatttaatat aattttttag cattaactct agttaaatca
    30721 cttttagata atgaaacctg taatcttctg ataggagaac ccttttctaa acatctattg
    30781 atcttttcct gtaacttctt acgaagaaga ggaactacat ctgctggttc agttgagtag
    30841 atatgttcaa cttctatatt ttctttatca acaactgcta atattatagc tttatcatta
    30901 taatatttat ttagtacatt atcagatata tcttgaaagt tccaagcatg atttttagaa
    30961 actttatatt cataaggatt accttcacta tcataggcat catgagcacc ttctcgtcca
    31021 ccttgctctg ggtttacttg atggtttaat tctaatgcaa ctattaattc ccaaaactta
    31081 ttacttgtaa gtaaattttt aactcctgtt aattcttcaa gttcacttga agctttataa
    31141 atagcactta gatatttatc ataaacacca ttttgagctg atgggattat cttacttgca
    31201 tttaatctag actctagttg attagggctg acatttatca tttttctgga attttgtata
    31261 ctttgagtat actttagtaa atattcacac attatatctc caggtataga gtaataccaa
    31321 tcaagctttc cagggtaagg aaaatcaaca tcatttatat gagcaaatat aactgcctta
    31381 gctttcttta attttgctat acttgtatcc gaaaaatcat taaacgtcca agaatggtta
    31441 gagctacttt tcttgaaatg tttatattca aactcatttc cgaattcatc ttttgcatct
    31501 ctagaaccac tatgtccagg tattagtatg tgattaagtg aattagcagt cataacttca
    31561 tatattttgc tattagaaag tatatcattt actttatatt ctttaaaaat accatcttgt
    31621 atagccttta gttcatttat tttctgtgtt attaaaacat ttgttatatt atctcttatc
    31681 atattatcat agctccttaa taaattagta tttttagata agtcattttt aattagattt
    31741 atcacataac tattgatgct atcattttta aatgctacta aatccaataa atctttatga
    31801 aaatcatcct tgagtcttat agttagtttt ttcaagctat cacctcggat attaattaga
    31861 tgtgccgtcg agataagtat aattaagcag gtttataaag tcaaggatta aaaaaattaa
    31921 attatagata tataaaagta tatatagcaa tatagataaa tagctaaact attaatcgtg
    31981 ggtattattc ttgcgattcg tctccaccaa ttttgaaaaa agaatgatga aatacttgga
    32041 aatactagtc tttgttgact agtatttttt attttggaga agaaatccac caaccgtacc
    32101 ccaaccacag aaaatgaggt ttttttgggg gtatattggg gtgcatttag gctgtataaa
    32161 aagtttttat ttgtttatta ttttatttgt ttattatttt atttgtttat tatgataata
    32221 ggtaacatag aattttaata tctactttat gatataatat ttttataaat gtaagacagt
    32281 ttaagttgtt aattagtaaa aatatatatt atataagctt gataggagtt gaggtttgta
    32341 atggatgaaa gaaaactaga attttctatt ttttgcattg aagctttagc agaaaaattg
    32401 aatatagatt cagaaggagt ttataaactt cttaaacatg atactgatat tttagataac
    32461 tatataatac cttgctatga agcactacat tctcaaagta aaggttacat agtggaagat
    32521 ttaatgcaag tacttaaaga aaagggtgtg atataatgat tgtatatcat ggttcatatt
    32581 gtgtggtaaa gacgcctagt attacctttt caagagattc tttagatttt ggaaaaggat
    32641 tctatgtaac cagtataaag acacaagcaa ttaactggac agagcgtttt aaaaaaagag
    32701 gtaaaaaagc atatttaaat agttattctt tagatattga taaattaaaa gaaaactgca
    32761 aggtaaaaat cttcgatagt tataatttag aatggcttga ttttataata gaatgtagaa
    32821 atatgagtga tatatatatg aaatatgaca ttataatagg tggtattgct gatgataaga
    32881 tttacaatac aatagaactg tatcaagata atcttattga aaaagatgaa gctttaaaaa
    32941 gattaaaata ctataaacca aatgaacaaa tatgtataac caatcaaaat gtaatagata
    33001 aatatttaaa gtttaggtat gaagaggagg tataatgatt atggaagcaa ataaaacatt
    33061 attacaaata ctatataaaa aaatcatatt agaattttcc aaacaaacag gtaagagctt
    33121 ggaagaaagt atggattatt tatatacttc tgaaacatat aaacttataa gtgagggtgt
    33181 atcagatatg cactgtaagg gtcatatata cttggctcaa gaattaatgt tagaaaatgg
    33241 attaatgtac cataagggct atccaagata aataattaaa actagatacg aaataaggat
    33301 acaatataat ataaaattaa tttgtatcct tattttgatt ttgtaattaa ctatatgaaa
    33361 tcacaatatt tatatgtatt tgatttttac aatgaaaata ttttatccaa cttttctaca
    33421 gcttcaactt tcttatcttc cataacttga gtatatatat tcaaagttat tgatatatca
    33481 taatgaccca ttaaaacttg tacagtttta ggaggaacat cattctcaaa aagtctagta
    33541 gcataagttt tcctaagacc atgaaacttt ataggctcta tatttaagct atttaatata
    33601 gactttaaat tccttaaagg tcttttatca tctataggga tgcctaattc attgcaaaat
    33661 acataattat tatttatata taattctcca gcctttaata tatctgtatt ttgatttact
    33721 ttatgctctt tcaatttatt taaaacatct tttggaatag gtacagttct gtaagaattt
    33781 gatgtctttg gcccttgctc taatacttta agctctctat ttccagtttt atctataaaa
    33841 tatgttcttt gaagagttct tttaactgtt aaattagact ttttaaaatc aatatcaaac
    33901 catttaagtc ctaataattc tcctaatcta agtccagtac caagagcaag taaaaataat
    33961 acctctaatt tatgcccatc tatagctttt ataaaactct tttgttgttc aagagtcaat
    34021 atttcaagtt tgttttcttt tttctcaact ggtatattta ccatagtaca atagttacgt
    34081 tgtataagac cttgtttttc agcttctgat aagcaggttt tcagtttagt gtttatttgc
    34141 cttatagtag gtatactttt attggaatca agcaatctat tgtaatattt ttgtatgtga
    34201 gatactctta agtactttaa tttaacattg cctaaatcag tatctttaat ataattacga
    34261 tagatgcttt catatctttc aaatgacttg ggttttaaat cttttattct atattcaaat
    34321 aaccaattgt aaaaccaatg ctctaaagtt attttatcat ctgaaatatc agagcttaaa
    34381 taatattgtt ttttataagc atctaattta tctttaactt ccttttgagt cttaccataa
    34441 aattcttttc tcagtggttt accatcttca ttatagccaa tcattatacg ggaacgccaa
    34501 ccatctttat attttccgat agagccttct ccattagctc ttttagttgc catttttaaa
    34561 cagctcctta tatgatttat agttatatcc atcaaagaat gtaatacatt tctctaataa
    34621 gaattttata acttcatcag cttctagtgt ttcatagaag tttagcatta atgtgttgta
    34681 ctcttgttta tgtttagctg gtattgatag aactcctata tcattattta taagtattag
    34741 gtttgaggta agtctagcag ttcttttgtt gccatcatag aagaattggc aataggacaa
    34801 ccataaattt attattatag ccttttctaa tgggttagtt attttattta tgatatttat
    34861 gtcatttaca aataaatcat ttaatagagt ggcttctatg cattcatatt gagtagtacc
    34921 agctatacct atattaccat ttctgaaatc tccaacgatt agagcttcat cttttgcaac
    34981 tattttatgt atagagcaaa ttgtatcttt atttaaagat aagttttctt tgttacttaa
    35041 ttttattaca taatcccatg atgattttat atttagtatt tgattttggt cacttaattt
    35101 atgtccgcct acggttatac catcaagtaa tgtttgtact tctgggaatg tgaaggggtt
    35161 attttctagg ttacccatat catatacaaa ttcacttatc attcttctaa tgtatgttaa
    35221 agtatatttt ttatctccat gaggtatatt agtagggaat ttatgtgtgt attttggtat
    35281 atatgattta ttcatgattt tagctccttt catatctaat ttttatttat taactatgaa
    35341 tgtataaaag ttaataggta aactcatatt ttgaaaatgt gcaatactaa tattcttcat
    35401 cctcatcttc aataagacca ttagagtttt cttgtataaa attttcaaca agaccattta
    35461 tcacccaatt tagatttttg gagaagtctt tgaagtcaga gaagtttagg ttaattggtt
    35521 ttattgtatc tgcattatct gtaatttcaa actttttttt atagtctgtt tttatatcat
    35581 aaccataatt ttctaatatg tgaattacta aatccaactc tttatcaaat acttttaaat
    35641 taatattatt atttgttaaa gtatctaaat cattttcttc aacaatgagt ttatttatag
    35701 caattgataa agcagtagct atttttctta tcatatctaa actaggcatt ctttgatttt
    35761 gctcatattt agctatagca tgaactgaac aaccaatctt gttggctagc tcatcccttg
    35821 acatatttat cgattttcta tattgtttaa tcttttctcc aattgtatta ttcatcataa
    35881 tttcacctta aatatatcat caaacaatga ttgtacttct aaacatgtaa aggagttatt
    35941 ttctagatta tcccatatca tatactaaat tactcataac tctcttagtg tatgttagag
    36001 tatatttttt atctccatga aataaattag taggggattt atatgtgtat tttagcatat
    36061 atgatttatt cataattgta actccattca tagtttttta tcattgcttt agatagtatt
    36121 gatattattt tatattttag ttagtagttt ttttatttct ttaagtgtat tttcaagctt
    36181 ttcatttttt tcctttagtt cgttattttc ttgtaataat ttaaagtaat cattattttt
    36241 aatagtaata ttttcttttt gagatattaa tttttttagt cgaatattac ttccattatc
    36301 tgttgagtca ctaaagtaat catagattgt ttctatactt tcttctgata gaccaatatc
    36361 taataattca tcaattgatt gttttgtaag aacatcattt ttaataattt cttttatgtc
    36421 atcaggatgt aatgaaaaat ctaattcagt attagatata caatctgaaa gtatccaatt
    36481 ataaacttga ttttcattta gttctaattt ttcaccaagt ttataatatt ggtctttatc
    36541 agaccaggca cctagcaata aacttgaggt aattctaata tcatcttcca taagatttga
    36601 aatagcattt aaaaattcat caaaagaata gtattcttct gggaataaag actgtaaaag
    36661 gtataaaaca tcttcattag tcattacttc attttcaata tcaaaataaa agatagtctt
    36721 ttcaattttc aaagctttag cgagttttga taaggtatct atactaggta ttcttttatc
    36781 attttcataa tttattagag catttcttga gatgccacta atttcagcaa gttgaatctg
    36841 cgttagctct ttttgttttc ttatcttttt taagtttttt ccaatttgca atattatcac
    36901 ctcgtataaa gtatatccca aaagtacaca aatgagaaca gaaaaataat atattcttat
    36961 taacagtaca catatatcga cattataatt aaaatgtaaa gtttacgaat gtaaactaga
    37021 aaaaaggagg aaaaagtatg ggaatcaatt taaaaatatt taggattaaa aaaggtctaa
    37081 agcaacaaga actggcagat agagttggta tatcaagata ttatttatca aacttggaaa
    37141 caggtaaggc taataatcca agcaatgatt tgatgattaa actatctaga gctttagata
    37201 caacagtaga agaattattt tttagtaagg aggattaaaa tatgaaagag aataataatt
    37261 tagaggtttt aaagacatta caagccactt taaacattat tgtaaagaat gaagaagaag
    37321 cacaagaaaa aaagcaagga tataaaatta ttgaaaatgc gaaaagaaat aaagctgagc
    37381 ttataacatg taaagaagca tctaagcttt atcctatagg agaatcaaaa tttaggcagt
    37441 tatgtcattc taaaaacaaa ggattccctt gtatatggat aaataatcga gtatacataa
    37501 taaaaaataa attagatgat tggtttattg aaaatgcaaa tggtatcaag ttatagtctt
    37561 aaagtaggtg tttgcaattg agcaataaag ggtggataaa gctttatagg gagttgttgg
    37621 ataaatcaat ttggaaagat attaaaccag aaagaaaggt aatattaata actctattac
    37681 ttatggctag tcataaagaa aatcatgttt ttatagataa taaaaagata aaaataaagt
    37741 ttggacaatt tatttcttca tatagcaaaa tagctgaaag atgtggtaaa ggagttagtt
    37801 atcaaatggt tagaagttgt ttgttggttt ttaaaaatgc tggttttcta acatacgaaa
    37861 caacaaacaa gtatatagta ataactattg aaaactggga gctttatcaa gagaatatag
    37921 aaaatcaaca acctaatcaa cagactatta acaatcagtc aacaacaatc aagaatataa
    37981 agaatgaaaa taatattata tatagtgaaa tcataagcaa atttaactca acttgtattg
    38041 aactttcaaa ggtcaagaaa ctaacagata caagaaagag gagaataaat tcaagaataa
    38101 aagaatatga taaagaaact gtattagagg ttataaatat agttagtaaa acaagttttc
    38161 tgaatggaaa taataataca ggatggaaag caaattttga ttggataatg aattcaagta
    38221 attttactaa gatactagag ggaaattata atagcaagga aattgtcata aataaagaga
    38281 caggaatata taatccagat gactcacaat taaagtggta aggaggttat attataaata
    38341 attcaaacaa cttaattaaa aatacagaag cagagcaatc aattaaatta cactaagtta
    38401 gttataaatc tttcatatat ttgtttttta agtatctcta atatcgttta aattacacta
    38461 agttagttgc agatatacca gaaaatatat ataaaattat gcgtaaacta tattgttatg
    38521 agtacaaata atatttacct attcattaaa aaacttaact aagaatttaa tttatattat
    38581 tgaagtatca ataattttgg aaagtagaaa attatacccc aataataccc caactacaaa
    38641 aatagacaat aaaaaataac cagaaaaatt gcttcaacat catgaccaaa tagccatata
    38701 cctatactta taaaaaccaa caaaacaatc aaataaccac ttataaattc tcctcggtct
    38761 ccaccattgg aatccacgaa aacatattga tttaatagtt aatatgtttt ttattttgaa
    38821 aaatcatata taatataaat taatagttta tttaaaaatt tcagtatgag cacaaaatta
    38881 tataattatt gggaattagt tataataagt aatttctagt aagaatgatt tgcaaaaaat
    38941 aataatttaa atacaaaaaa ctaaaggata gaagtcataa aattaacttt taacctttag
    39001 ttatataaat aaaaaattag ttattactaa attaaaatta gatttttaaa gaacccaata
    39061 tctctattat ccatatcacc aattaacatg cttctatcaa gtaataaatt atactcctca
    39121 cagctagttt caggcaataa agtacatgaa tggcatgctc ctagatttaa agaatttcta
    39181 ccttgaccag tactatcaat acatattggg tcattagaac accaaagagc acgattaaga
    39241 gcactattta atatagatgg taatgaatca tgcatacctt gcctaactaa tcctcctaaa
    39301 gtaccttcag agtcaccact tgcagtgtaa actaaaattc cagacataac tttttcattt
    39361 tcggaattat tacaatatat acgttcccta agtgaagagg tagcatatcc acaaacaaaa
    39421 ctaagttctc ttataattaa atgagctaaa gtatgtagta ataagaattt aggtgtaatt
    39481 tttctataag ctttgtcatt aacattaata tctttattat atctagaatt taaaatagat
    39541 gcattatcct gtattttagg attagatgaa atccagcttt ttaccatatc attatttatc
    39601 tctatgaaaa taccttcacc tctaacctca gatgcaggat aaaaagattt tttacaatct
    39661 ctagatctta aacttataac cttagtatca ttacagctat catctatatc ttcttcacca
    39721 aatatattat tatcaggtgg attgactcta ctaaacccag ttaatgccct tacttctcta
    39781 agtcttttta ctaacactat tttactgaag tttggaatat cataatcaga tagattttgt
    39841 tcttctattg taaagtcatt agttttttta gatttagcat ctatatttcc aattaaagcc
    39901 tcatattctt cttctctata ttgatttcta gttgttgttg atggtgaaga atcttcaaaa
    39961 ggcaataaat ctttcaaaac tatggatact ttatcagtat caaaacctgt ttctttagca
    40021 atatcactgg caaagtcctc aaaatcctca tccataaagc gttctaactt attcttttta
    40081 atagcacgat ctaagaattt cttaaatgta tcataacctt tactagatag cactttagaa
    40141 ttaagagtat cagagtatgg tggtataata attgaacttt ctatcttagg ataatataca
    40201 ttagaggcac ctctttgaga agcaaatgaa tatttagtgc atttatttac ttttttatta
    40261 taccaaggcg aattaccaga gcatttaaac atttccttat attcactagg tacaccataa
    40321 tcattagatt tagaagattt taaacgcata aatgcgccct tcatattagc tttagctcca
    40381 cagctacatt caagttctat accttctaat cctaggttac tttttccagt cttcattaat
    40441 atttgaggat tttcacaaat ttcttttctt ccagatttat ttctaaaatg aacccatttt
    40501 acccatggaa aatcatcaat atgaccatgt tcacaaatag taattatacc agtaggtact
    40561 aattgtttag tagacttttt tgtgctacaa gtaaggcaaa taggtatttt cataggttta
    40621 tctttatgag catttttata aagtttctcc caatcttcta taggcataaa ttttctacat
    40681 tttggacaaa agtaccatct aggaaaccta acaaaaggaa cagaagatct atcatctata
    40741 gttggaggtg ttcttagttc attaaccctc aatgtagcct gaagcctatc atcttgtatt
    40801 ttaattgtat tagtccaata atttggatga gcagtcatta atgtttgatc aataaaatct
    40861 agcatagctc ctggtccaaa aacacttgta gcttgattta ttcttaaatt atgagtttta
    40921 tcatctaatt ttatagacat attaattttc ctccaattct ataatttcaa cagtacattg
    40981 tttatcaaca tttctcatag aatttaatgt ttgtttttca tttgtaataa catccttatc
    41041 ataaggaatt aaaagattat tatatatttt attatcaaat ttactagggc gcttataata
    41101 atgcaataaa ttatctttat cagatatttg agctttatca tgccataaat ccattataat
    41161 atctaattca gatgaaatct cagatttaaa ttcatttact atattatcat cagcattttg
    41221 attattttct ctaaatatat cgatagatct atctattatg aagtttttta aatcttctac
    41281 accatccata ttttttgaaa atcgacttgc aagatcatta gcttgaagtc ctaaaatatg
    41341 tctaatcata cttattaata ctgcatgcat gcctctttct ctagctggtt cagagaaagg
    41401 tgtaatacta gtaggttcaa caaatttata gaatgattga tgataactaa agaactgttc
    41461 ataatgagat ctatctcttg tttttgtagg attatataat gtaaatataa gtccttcatt
    41521 acttctacca actctactgc tagcttgtat gtattcagat gttaacttag gctgaccaac
    41581 tactaccatt aaatttaatc tatcaacatc aacaccaact gaaatcatat tactagctaa
    41641 tagagtatcg acagttaaat tattagaagt aagagtatta gatagttcat ctaaagttgt
    41701 atttatctta gtagaagtaa gtctactagt aagctctctt tgttcattta ggttccttaa
    41761 tgattgataa gggtgggctg agactctttt acataatctt tccatgttgt cttttatatc
    41821 gtcatttaaa agtgtgctac acttacctaa ttctctgata ctattaaaat aagatactag
    41881 tgtccaatat ttatccaata aattcaactc tacattctcc atcattttaa gtctttggag
    41941 taatgctgac ataagtctaa cctctacagt aacttgagtt tttccagatg gaataacacc
    42001 tacatatagt cggccacgtt ttttacttaa aggcatctct ttaacaaaga aagagttact
    42061 agatgttaat cctggaggtg gaaactgagc tacattacga ttatatagat ttttaacttg
    42121 attttgtgct tttcttatag tagcagtaga agctataatc ttaggtttta tacccttata
    42181 gctacacata gcatcaagtg cagtttcata taaacctacc atagtaccta aaggtccaga
    42241 aataagatga agctcatctt gaattattaa ctcaggagat aagttgctat tatcatcatc
    42301 taaagcaaaa agactagcta cttctgtagt ccaagttatc attgcaaatt tatcaactgt
    42361 accaaataaa agtgttggag ggttattata aatctcctca tctacaactt gtataggtaa
    42421 catatcttca aatgaacaat cttcttcagg acagcataag tagaatttct taggtctaga
    42481 tttaattcta tagccccaat caccaacagc ttttttagaa gttttatccc attctttaac
    42541 aagttttgtt ccacaccaag gacattttag taattggaat ttattatatt ttgattttaa
    42601 attatctaac tcatattcac tatttatact agatctaaac ttaatgttat tacctgttaa
    42661 gttattaagc atttcttctg cctcatcatt aaagtttgga gtttgatcac tcccaatcca
    42721 caaaccaata gaaatctctt ttttcccaag ttcagactta ttttcttttc taataatttc
    42781 acaggcacaa attaatatac ttgctcttat gaattgttgt gcagccaata gccttagtgt
    42841 atatctcatt atgatagctg taccgttacc attttctttg tttcttattc ttcttaagaa
    42901 gatagtaaaa gcagatagac ctaagtaggc ctccgtttta cctccaccag tagggatcca
    42961 aagaaggtca actaaatctc tatgtttaga gtcaggattt tctatcgaag ataaacatgt
    43021 aagtatataa gcaagctgga atggtctcca agcagaatta tcatttgagt aatctatata
    43081 gtcaaggtta ttagatccag ggagtctatc cgtaatatgt atagaatgac atctttgcat
    43141 aagtatagct ctattcatta gagaaaatgc tctgtatact tcatcattag acttaattaa
    43201 atttatacct tctagcattc tagacttagt atctctgcag ttttttatat gctcatatgc
    43261 tacatcttta aaaacatcac taagatcttt tgaatcttct tctagatcat ctatccaaat
    43321 atcataagca tcaacaaact tataaagaaa atatattttc ttatctttac taagatcaga
    43381 gatatctgat aaatttttca tagacagtaa ttctctagaa acatcactta attcatctac
    43441 attaaagtcc aatttaggta cttcaaaatg aggaataaac tcagtacaaa gttttattaa
    43501 agcatcattt tgaatatctt gatctatagc tacaccatga ccagtagcaa aagttttttt
    43561 atttcggtaa agtaagtcta aagatatttc ctcagaatct aactttttaa aagtatttac
    43621 tgatccagct gtaaaaatat ctataaactc taaatcattg ttatcttcag ctaataccat
    43681 tagttcaggc tggaaaatag tattattata tttcttaggc ccagattgac tattaaccag
    43741 catacaagta atactaacaa agtcatttga ataaattctt ttagataaaa ctatatctaa
    43801 atctttagat ataggttttt taatatagtt tttatccata tctattgata ctattaagtt
    43861 ttcatgtgga acacgaacat aaccaccagt cttattctca tcatcagggc attggttagc
    43921 taacttgtat aaaacaaatt tagcttcagc attatcaagt aaatcatgct tttttagtaa
    43981 attataaacc ttctcagatg ttagtttttg cttaagatat aggaatgatc ccctaagttc
    44041 tacatattca ttaattgtat tgctagaata gaagtcgtca ccagtatatt taacatgaca
    44101 gtctgtatat aaagcatttc tataagtagc ataatttacc ctgaaattta ttacttctgc
    44161 atcttttttt aggaaaaaag tcatacccat agaagaaggt aaagtttcat tagacatact
    44221 tatagctcta tctagttcat tttgtgtatc atctgatgta aatacattat cttcaccatc
    44281 atattcagat gtattactta catcaatatt gttattaagt ggatataaaa tacctgttga
    44341 ataacgctct attggactat ctgttattat ttcatatttt atattttcac aaagatcttc
    44401 cgaaccaggt ccaagaagtt cttttcttac aagatctagg gttttatctc ttagttcact
    44461 acaattcata tttacctcct atttatttaa taaacactat ataagtctaa aactttgtta
    44521 acaaggtggg gtgacagata cttatcacaa ctgtgtattt ctcgtatttg tacaggtgta
    44581 acataatcat ttgtattccc aagcatactt aaaacaactg cagacacttc atctttaatt
    44641 atatctgatt tactgctaaa gaaattatca gatacaaatg tatctataga tttttttatt
    44701 ttagtgtgca aagagcgtat acttttagta gcatcatata cctcggtatg atctaattta
    44761 gaatctataa ctttaaatat cttttcgaaa gtttttaaat atataggacc tattaatctt
    44821 ttatttgtta cccagttttt gattgttata ggattaatct tttcacctaa aacagctaaa
    44881 ctgtttgata catcattata attatattta ctataagata tatagttttt aagttctgat
    44941 ttccatgaat tagttaattt ataatcatca agataatgag atggcaataa attaagttct
    45001 aatagctttt gtataacatt aataactaat tcaccctctt catttaagta atcatcaaca
    45061 aataaaaacc agtcattaac tttagcctct gaaagtttct ttttcctaac acttttttta
    45121 tcccaatcta ttgaataggc tattgccttt tttgtacaga atatatactc attattatca
    45181 gaaatcatga atgatgtaac atgttctttt ttagagttgt aaatcccttg atttatagat
    45241 agagataata taatttcatt taaatttata tcaaatttat aattagatat atattcatcc
    45301 atagaatcat catcagaaat aggactatcg gtagaataag cttctaattt attggtttca
    45361 taagttggtt taaaaggttt tatatcaagt aagttatata ttttattatt tttttcaatt
    45421 ttatttcttg cagaatttat ttgattcata tagtaattat atttggataa ttcactatta
    45481 tataaataca gtgtaatatt tataatcaaa tcactattta aaatatctaa atatttcaaa
    45541 tcatagaatc caaacataat aagatttttt attgtttttg aatctttaaa tttttcagca
    45601 gaaataactt gtaaatttat atttctagat tttaataaat ttttaagtat aatagcttca
    45661 tattgttttt gtagcaatat aacaggtgta tttggtgtaa atgttttgta tttatatgaa
    45721 gatactaatt tatttagtgt attgaatttg gtattattat cagataattc atttataatt
    45781 ctttcaaatt tgactataac atcatcaaat aatgcactta attctaaatc tgggctagta
    45841 cttgaaatta aatattttat attttctagc ttcgctgaag gagttgctat atttaaattt
    45901 aaaagcttta tagtttcttc taaaaaatct aggttaaaag gtgtattttc taagatttta
    45961 agaagagagt aactacacat cataagtgag ttttttatgg aaatatcaaa atcatactta
    46021 gatatgtaat aaagtgattt tctagcctga tgtatcaagt cagagatatt agaatcaaca
    46081 gaagtagtac atatattttt atttataagt ttatctagcc ataatttctg aaaagtagag
    46141 gtattttcaa aatcttggct ataggttaac agaacatctt tagaccaaga gtatatattt
    46201 agattatcta tatttggtaa atctaatata ttgcttatat ttgaatttga ggttacaata
    46261 tttacacttt ctatcctttt tctatttaaa attctttcaa aatcatttat atgcttataa
    46321 attggataat cacctattat aaatacattt tttatagact tatcagattt aataatttca
    46381 aatgcactag aaatattagt tgtaaattta agtaaaggta tttgcttagc gtgatttcca
    46441 ggataatcag tttcggcacc agttgatgaa atatatgaac atggaaatag ttcagtaaac
    46501 ttataagtat tattaatatc ttttatttta atattactaa ctaagtcaga tatatcatct
    46561 ttacaaggca atataatcag tgtagaatca tttattactc cagtcatatt ttttaagtct
    46621 gaaaaaccta aagatgatag aacattcttt gtttgaaaag tcttagatgc tggagttttg
    46681 cccatagaat tgacttctaa atgaccatta taggttataa gcatataagc cgagtcaata
    46741 ggtataagtg ttgtatccgt tttattttta tgtcctaaag ttatgtgtgt attatttata
    46801 tttttaaata taactatttg acccttgtag agcaggggca tacctttatc gagttttgta
    46861 agaatgtgat tatttgggtt taatgtatta tttaagtatg agtttatagc tatattcaac
    46921 aaataaatag catatatgta tttgtcatta taattgataa ttaagttatt atttttttca
    46981 tttataatat aatttaatac attaatatta tcttgataat acttagaatt tgttagcctt
    47041 tgtttattaa aataaatttc acatatatca ttatcaagaa tataatggat atttgtagtt
    47101 aacatttaaa tctcctttag tagttactat tgtttattag aatagttttt attctatatt
    47161 ataattatat tactaaaaaa taatatatgg tatataatat aaaaaaagat tgataaataa
    47221 ataaaaaata tattatgtta tttatttgaa tagattatcc agaagacata aataagaaaa
    47281 ataatgtgta agtaaaaata ttagaattta ttaaaagcta ttacaagtat aagaaatttg
    47341 aaatgattat tcaaaaattg acatgtgaaa aaaaatatga tatgctgcaa taaaagttaa
    47401 tgtgaggtat gtcagaatgt ataatgttat agatcttttt gcaggtgcag gtggtttgag
    47461 cttaggattt gaaatgacta aaaaatttaa tatggtggct tttgtagaaa aaaatgataa
    47521 cgcagcaaaa acataccttg aaaatcatcc gagtgttaag cgttattgtg atattaaaag
    47581 attagatttt caagatatat taaacagtgt cgataagata gatgttgtta taggaggacc
    47641 gccatgtcaa ggtttttcta atgctaatag acaaaaaaga aaaattataa atggaaataa
    47701 tgaattagtt aagctatatg ttgatgctat tgataaatta aagccaaatg tatttgtaat
    47761 ggaaaatgtc aaaactatat cttcaaataa gcattcattt tatttgacta aaaaagataa
    47821 aaatcatata ataaataatt taaaattaaa tatttataat aaagatagtg ttttatatga
    47881 tagaactgag tatataaatg aattatttaa tattattagt atagaagata ttgaatcata
    47941 tattatatca aatatagact taaaaaattt gaaaataatt attaagaaaa aaaaggatat
    48001 atgtgattat ttaaataaaa tatctaatta taagaaagta aaatctacca tagaaaaatt
    48061 ggaaacaagg ggaaaaatgc caaaatggta tttagaatta ataaataaag taaaaaatat
    48121 attagaaaat atgttatata atagaaattt atcagatgaa caaatatttt atttgaactt
    48181 attcttggat attcaaaatt tatttttagg aatatatgag ttaaaatctg aatctgtaat
    48241 tttcgaaaat acacttgatg ataataaaat tatagctaaa atggatactt acattataat
    48301 agattatata aatgcatcct ttgagtatct aggatatgta ttaaatggaa gtgcactaaa
    48361 ttcaattgat tatggagtac ctcaaaatag agaaagattt gtattgatag gagcaaaaaa
    48421 agacttctta aaaaataaaa agatagaaac tcctaagcct atagttggag aaaattatgt
    48481 tacagtaaga gatgcaatag gcgatttagc tgaatatgag gcttctaaag gtagtatgga
    48541 ttattgtttt gaaaaaaata gtaataatat tgaaaaagat ttttatagaa agataatatt
    48601 ggatagcaat aaaatataca atcatgtatg tacagataca agaaatatcg cactaaaaag
    48661 atttgaatat ataaatcaag gaaataattt tcattctttg ccagatgagt taaaaggaac
    48721 ttatgctgat ccagaaagaa ctcaaaatac tatttataaa agactagtat atgataagcc
    48781 atcagatact gttgtaaatg taagaaaatc tatgtggata catcctatta aaaacagagc
    48841 tgttagcgca agagaggcgg ctagattgca gtcattccct gatagttata agtttttagg
    48901 tacaaaagat tctgtatatc aacagatagg aaatgcagta ccacctttat taggtcgagt
    48961 agtagcagaa aagatattaa atttatttaa tgatgaacca aatgagtatc ttagagatgt
    49021 gtttgaagca ctagatggat agttcaaata aaatagtggg tattattctt gtgattctta
    49081 tccaacatta atccacgaaa taaggactgt cgaatgagta attttaaatt gctgtgcgac
    49141 agttcttttt ttacttgctt ttcattaatt tatatttatg caaaatttga gcttgaaaac
    49201 ggtcataaga aataaaggta taaaaataca attttaaaat agaataattt acaaagtgta
    49261 agattaaaaa atatttttga gaggatgaat atacatacta catttattaa tgattatctt
    49321 atttgtgaaa cttagaaata ctataggcta acactacagg tataaactct gaaagaatat
    49381 tttgattgcc tttgattaga attcttttaa agttcatact atgttttatt tgggtgaaag
    49441 ctccttatgc ctgaatactt ctattcattc taagttcaca tccttgtgta ccagtgattc
    49501 tttctaaatt atcttaatgc caaaatcttg ttcgcatttc aaaataaagt caggtagcta
    49561 cttcaacatt ttagttatat ttcttcaata gtttgtctcc aaacaaaggt acatctatta
    49621 gaaacagatt taattttatt tatatcaata aataatatat actttgaaac ttcattaata
    49681 tcaccaagat aatttataag ttaagctatt atatactatc ttcaccaact acatcctcca
    49741 tagttttaca ctttcttaat tcagcttggt aatctaaatc tgtaataatt atttctcttt
    49801 gatttttcat ttctttgcct acaaaagttt aatataaata ttattccata cttaccaatt
    49861 ttacgttcaa aaaagactgt agataagtct gcatttacac aaacctactt ataggtctct
    49921 tttttgaaat taatttagca tgaactatag taagagcatc tttaaattca tagttacaag
    49981 tatacaaagt tataatttta tctgaaatat ctacctttat atcagatttg tgtactgatt
    50041 tttaggttat ataattcaaa gaatatttaa actcatcatt attattaaag tgtatactta
    50101 aataatctct gttaattaag tcagcagtat atactgaaaa aatttcataa gtataagttt
    50161 gtactttata atttatttct actaagttat tttctttgaa aaatacttca tcagtatagt
    50221 tttctagttg accaaac
//
LOCUS       DBGJFU010000013        49628 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.013, whole genome shotgun sequence.
ACCESSION   DBGJFU010000013 DBGJFU010000000
VERSION     DBGJFU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.013"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            152..826
                     /locus_tag="ACOIAS_000759"
     CDS             152..826
                     /locus_tag="ACOIAS_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700479.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKGALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREKRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            814..1974
                     /locus_tag="ACOIAS_000760"
     CDS             814..1974
                     /locus_tag="ACOIAS_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2700480.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASTLTTFSYILLNNLYNSIQD
                     KYLKTPINTGFITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2212..2718
                     /locus_tag="ACOIAS_000761"
     CDS             2212..2718
                     /locus_tag="ACOIAS_000761"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGZ2700481.1"
                     /translation="MTFSRIVFALLMLSFTFCSLPFWICYFWCGISDIFDGLIARKLR
                     QQSGIGAKLDSIADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYR
                     TFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELN
                     RDIKSIFM"
     gene            3004..3672
                     /locus_tag="ACOIAS_000762"
     CDS             3004..3672
                     /locus_tag="ACOIAS_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700482.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3675..6143
                     /locus_tag="ACOIAS_000763"
     CDS             3675..6143
                     /locus_tag="ACOIAS_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2700483.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTIAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFATIFGSIAGYVSAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDVNL"
     gene            complement(6382..7551)
                     /locus_tag="ACOIAS_000764"
     CDS             complement(6382..7551)
                     /locus_tag="ACOIAS_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGZ2700484.1"
                     /translation="MLYQVVDNNGNILYGTNPKKLFKTKEELFNNFINKTVRKGGYIH
                     TVPIKGDNGKIEGAVILFYQVKITFANIRGRFVFAVIIMALFSPFLYIVGFTRWLSKR
                     FVKNINQPLHLLIDASKKIKEKDLDFEIDYYSDNELGKLCSAFSEMKDELKGSLSAQW
                     KMEQERVEMVEALAHDLKSPLSIILGYTDALIGNNTDDNEKLHRYLTVIRENTEKSAA
                     LVQKMQYTSDLEKSNIQLNLVPINLPEFLRQKVQDYELQAHQKEVELILKMQGNIQSP
                     IQIDVDRLTRIFDNIISNSLQYTPSGGNISITVKDEKNCISYEICDSGRGFSSKDLKK
                     ALDKFYRGDEARQTKGGHSGLGLYIVKQLVEQLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7825..8517)
                     /locus_tag="ACOIAS_000765"
     CDS             complement(7825..8517)
                     /locus_tag="ACOIAS_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700485.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8572..9348)
                     /locus_tag="ACOIAS_000766"
     CDS             complement(8572..9348)
                     /locus_tag="ACOIAS_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HGZ2700486.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLFMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9345..10115)
                     /locus_tag="ACOIAS_000767"
     CDS             complement(9345..10115)
                     /locus_tag="ACOIAS_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HGZ2700487.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10112..10816)
                     /locus_tag="ACOIAS_000768"
     CDS             complement(10112..10816)
                     /locus_tag="ACOIAS_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HGZ2700488.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11071..11583)
                     /locus_tag="ACOIAS_000769"
     CDS             complement(11071..11583)
                     /locus_tag="ACOIAS_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700489.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGYVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11909..12166
                     /locus_tag="ACOIAS_000770"
     CDS             11909..12166
                     /locus_tag="ACOIAS_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700490.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12309..12935
                     /locus_tag="ACOIAS_000771"
     CDS             12309..12935
                     /locus_tag="ACOIAS_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HGZ2700491.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13182..13961)
                     /locus_tag="ACOIAS_000772"
     CDS             complement(13182..13961)
                     /locus_tag="ACOIAS_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HGZ2700492.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNRCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14029..14490)
                     /locus_tag="ACOIAS_000773"
     CDS             complement(14029..14490)
                     /locus_tag="ACOIAS_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2700493.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14466..15143)
                     /locus_tag="ACOIAS_000774"
     CDS             complement(14466..15143)
                     /locus_tag="ACOIAS_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2700494.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15376..15762
                     /locus_tag="ACOIAS_000775"
     CDS             15376..15762
                     /locus_tag="ACOIAS_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2700495.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15765..17564
                     /locus_tag="ACOIAS_000776"
     CDS             15765..17564
                     /locus_tag="ACOIAS_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HGZ2700496.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSSNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILKDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLNKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17919..19793)
                     /locus_tag="ACOIAS_000777"
     CDS             complement(17919..19793)
                     /locus_tag="ACOIAS_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HGZ2700497.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19798..21462)
                     /locus_tag="ACOIAS_000778"
     CDS             complement(19798..21462)
                     /locus_tag="ACOIAS_000778"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGZ2700498.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(21476..22297)
                     /locus_tag="ACOIAS_000779"
     CDS             complement(21476..22297)
                     /locus_tag="ACOIAS_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGZ2700499.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22396..23160)
                     /locus_tag="ACOIAS_000780"
     CDS             complement(22396..23160)
                     /locus_tag="ACOIAS_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2700500.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23157..23906)
                     /locus_tag="ACOIAS_000781"
     CDS             complement(23157..23906)
                     /locus_tag="ACOIAS_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2700501.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24410..25015)
                     /locus_tag="ACOIAS_000782"
     CDS             complement(24410..25015)
                     /locus_tag="ACOIAS_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2700502.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            25194..25832
                     /locus_tag="ACOIAS_000783"
     CDS             25194..25832
                     /locus_tag="ACOIAS_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2700503.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            25964..26626
                     /locus_tag="ACOIAS_000784"
     CDS             25964..26626
                     /locus_tag="ACOIAS_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700504.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            26626..27807
                     /locus_tag="ACOIAS_000785"
     CDS             26626..27807
                     /locus_tag="ACOIAS_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2700505.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(27988..30648)
                     /locus_tag="ACOIAS_000786"
     CDS             complement(27988..30648)
                     /locus_tag="ACOIAS_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2700506.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(30645..31328)
                     /locus_tag="ACOIAS_000787"
     CDS             complement(30645..31328)
                     /locus_tag="ACOIAS_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700507.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(31672..32625)
                     /gene="blaCDD"
                     /locus_tag="ACOIAS_000788"
     CDS             complement(31672..32625)
                     /gene="blaCDD"
                     /locus_tag="ACOIAS_000788"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HGZ2700508.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(33166..35280)
                     /locus_tag="ACOIAS_000789"
     CDS             complement(33166..35280)
                     /locus_tag="ACOIAS_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGZ2700509.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
     gene            complement(35364..36200)
                     /locus_tag="ACOIAS_000790"
     CDS             complement(35364..36200)
                     /locus_tag="ACOIAS_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700510.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            complement(36784..37146)
                     /locus_tag="ACOIAS_000791"
     CDS             complement(36784..37146)
                     /locus_tag="ACOIAS_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2700511.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(37380..37844)
                     /locus_tag="ACOIAS_000792"
     CDS             complement(37380..37844)
                     /locus_tag="ACOIAS_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2700512.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GLYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(38273..39694)
                     /gene="nhaC"
                     /locus_tag="ACOIAS_000793"
     CDS             complement(38273..39694)
                     /gene="nhaC"
                     /locus_tag="ACOIAS_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HGZ2700513.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(39724..40788)
                     /gene="orr"
                     /locus_tag="ACOIAS_000794"
     CDS             complement(39724..40788)
                     /gene="orr"
                     /locus_tag="ACOIAS_000794"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HGZ2700514.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(40773..42182)
                     /locus_tag="ACOIAS_000795"
     CDS             complement(40773..42182)
                     /locus_tag="ACOIAS_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HGZ2700515.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(42182..44386)
                     /gene="oraE"
                     /locus_tag="ACOIAS_000796"
     CDS             complement(42182..44386)
                     /gene="oraE"
                     /locus_tag="ACOIAS_000796"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HGZ2700516.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIIMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(44380..44754)
                     /locus_tag="ACOIAS_000797"
     CDS             complement(44380..44754)
                     /locus_tag="ACOIAS_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HGZ2700517.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(44832..46262)
                     /gene="ortB"
                     /locus_tag="ACOIAS_000798"
     CDS             complement(44832..46262)
                     /gene="ortB"
                     /locus_tag="ACOIAS_000798"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HGZ2700518.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(46255..46563)
                     /gene="ortA"
                     /locus_tag="ACOIAS_000799"
     CDS             complement(46255..46563)
                     /gene="ortA"
                     /locus_tag="ACOIAS_000799"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HGZ2700519.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(46684..47751)
                     /gene="ord"
                     /locus_tag="ACOIAS_000800"
     CDS             complement(46684..47751)
                     /gene="ord"
                     /locus_tag="ACOIAS_000800"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HGZ2700520.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKINFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(48007..49407)
                     /locus_tag="ACOIAS_000801"
     CDS             complement(48007..49407)
                     /locus_tag="ACOIAS_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HGZ2700521.1"
                     /translation="MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMN
                     RHDILTNSFFDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPID
                     IKGEFVGALEISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAI
                     AKSKKACKSDASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGIL
                     FGTSKGGFTGAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTK
                     DIPIDVRIIATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFI
                     SKYNKKLNKKVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINL
                     NDYYTLKENKESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMN
                     RQNLQYKIKKYNIKIL"
BASE COUNT        16820 a         8359 c         5911 g        18538 t
ORIGIN      
        1 aataatttca tccttgattt atttaatata taaaaatata ctaactttaa tgattaagtt
       61 ttgaaatata actaagaatt tgagatttta ttgagatttt tatactaaag tatacatact
      121 atacaagctt aatttagaag ggagcattct tatgaacaat aaaatactcg tcgtagatga
      181 tgaggaaggt ataataacac tcttaaaaga ttactttgaa atgaatggtt atgaggttta
      241 cactgctcaa agtggaaaag gcgcattaaa aaaaatatcc aaaaatccag atattattct
      301 acttgatata aacatgcctg aaatagatgg aattgaagtt tgtaatagaa ttagagatta
      361 tgtatcatgc cctattctat ttcttacagc tagaattgaa aatactgaca agataaatgg
      421 ttttcgtgct ggtgctgatg attatattgt aaaacctttt gatttagacg aacttggagc
      481 tagagtatct gctcatttaa gacgtgaaaa aaggaagtca aatcaaacta aaattaaatt
      541 ttataatgat ttaacgatag attacgaaaa aaggttaatt tgtatagata ataatcagat
      601 ttcactttca aaaaaggaat ttgatatagt agaacttctc tctatgaatc ctggacaagt
      661 atttgataaa gaaaaaatct atgatagagt ctggggatac aatagcaatg gaaatagtga
      721 tgttataatg gaacacatta gaaagatacg aatcaaacta tcaaaacata ctctggaaaa
      781 ttatatagaa acagtctggg gagttggtta taaatggata aaataaataa tatgtcatta
      841 aaaaaagctt tctttgttct tactttatct tcactaatta tagcatcaac acttactact
      901 ttctcataca ttttgcttaa taatctatat aattctatac aagataagta tttaaaaaca
      961 cctataaata cgggttttat aaccatagtt caaaacaatg tgaaccaatt tagtgattat
     1021 gataaaacgt tacttaatat aatcaacata cttcagcttg tacttccact tatatttttt
     1081 ataggacttt tattattagc tgatataatt ttttacaagg taaaattaaa aacacccata
     1141 gaaatactca ataaaggtgc aatggaaata tccaacaaca atttagactt ttgcttagag
     1201 tataataata atgatgaatt aggtaatcta tgtaatgctt ttgaaaaaat gaggtctgaa
     1261 ctaaataaaa acaacataaa gatgtggact atgattaatg atagaaaaca acttaatgct
     1321 gctttttctc atgatttaag aaatccccta acagttctaa aaggttactc aaactattta
     1381 acaaaataca taccaacagg taagcttagt gatgagaaga ttttatcaac cacacaactt
     1441 atgtctgagc atattaacag aattgaatac tatgtagaaa atatgagtaa tgctcaacga
     1501 ttagaagatt tagttgtttc aaaatctatg tcgaatataa acaagtttat agaaaactta
     1561 gatgaaaata tatctatcat tgctaaacaa gaaggaaaat cttttacact taaaagtcaa
     1621 ataaataata taaatctatt ttttgatgaa aatatcattc ttagagtcgt cgaaaatata
     1681 atatcgaatg cttttagata tgctagaaat aatgtatcca tacttatata tcttgaacaa
     1741 gaactcctta cctttgtaat tgaagatgat ggtataggat ttagtaagga atctctaaaa
     1801 ttagctttaa gacctttcta tagagataag actttaaatg acagcaatgc acactttggt
     1861 atgggtcttt atatctcaaa aatattatgt gaaaaacatg gtggctctat ttctattgaa
     1921 aataattcta ctaacagtgc taaaattact gctaaattct caacaagaaa ttaaaatgat
     1981 tttgtaagct ttcactgcta ttatataaaa tttgttagtt tagaatttat aaaatgtatt
     2041 tcaatttata accaatatat atactatttt gtagaaattg taagagttgc ttcaattata
     2101 atattttggc aaaaggcatt tgaaacaata atagaaatat atatttaaat atgtagaata
     2161 gtatgaaatt agaattttgg aggtgattat attaaaagtt ttgctaataa tattacattc
     2221 tctcgtattg tgtttgcatt gctaatgctt agttttacat tctgttcact gcctttctgg
     2281 atatgttatt tttggtgtgg aattagtgat atatttgatg gtcttattgc ccgaaagttg
     2341 agacaacaaa gtggaatagg tgcaaagtta gatagtattg ctgatttcat tttttcggtt
     2401 acaattttgg ttactgtcgg aaaaaatatt gctcttccca tgtatctatg gtggtgtatt
     2461 gttgcaatag cattgttacg gttgattagt tacagtattg gtttttttaa gtatcgcaca
     2521 ttttcatcgc ttcatacttt gatgaataaa atcactggaa tttctatctt tgcgtttcca
     2581 ttactctata tttgctttgg tcttgatgtt gcaggaatta tgatatgtat cgtggctttt
     2641 ctttcttctt ttgaagaaat agcaattatg ataaaatcaa aagaattaaa tcgtgatata
     2701 aaaagtatat ttatgtgaaa acttagaatt tgtgacaaaa ataatttata taataatgag
     2761 gaagtaaaac ataaaaaatt atccacaagt ttatgtgtaa aatagtttta aagcaataga
     2821 gttgtattgt aaagccttta gtgcataaat tacattttaa attaaaaatg atactgaaac
     2881 tgcttatcag cactgcgaat tatccgcaaa tagtgaagac tttttagatt taagctaaaa
     2941 gttgggaaaa aattgagttt taaaacttac actcttctta tatagaaaag gagagtgttt
     3001 tttatgatta taaaagcaaa acaattatca aaaatatatg gttctaataa caataaagtt
     3061 atcgcactta ataatgtaaa tttagaaata aactcaggtg aatttgtttc tgtaattgga
     3121 ccttctggaa gtggaaaaag cacactttta catattttaa gtggattaga taatcctacc
     3181 tctggacaag tgttacttga tgataaagat atatataaac acactgaaaa agaactatct
     3241 gcacttagaa gaaaatcctt tggattcgtc tttcaacaat ttaatctttt acctgttctg
     3301 acagcatctg aaaatatttc aatgcctgta cttctagata aaaaacaacc tgataaagaa
     3361 tatttaaatg aaatctcatc attacttgga attgcagata gactcaatca tcttccccat
     3421 gaactttcag gtggtcaaca acaaagagtt gcaattgcta gagcacttat tgcaaaacca
     3481 gatataatat ttgctgatga gcctacagga aacttggaca gtaaaagtgg cagtgaagtt
     3541 atgaatcttc ttataaaaac ttcaaaacag tttggaaaga ctttagtggt aattactcat
     3601 gatgatagaa ttgcaaaact tgctgataga aaaatttcaa ttattgatgg tgttcttatg
     3661 gaggtgaaat agaaatgaag aattaccttt cactttcaat aaaagaatta aaaagccaga
     3721 aacttatgac tacattcatc attatagcaa tagtactatc aactattatg acaactgtgg
     3781 ttggacagtc aattggtatc ctacaaaatt taagaattga acaagcccgt tcttttaacg
     3841 gtgatagaca tgtatccttt caccaactta ctaaaaatca agttgatgac ataaagaaag
     3901 atgatagagt ttatcaagct ggtacttcaa taactcttgg cagtagtaaa ataaaggatt
     3961 ctggtatctc tgtacttgta aaagagtatg ataaaacagg actttccaac taccccaaat
     4021 taatgaaatt aaaatctgga aatttgccta aagataaaaa cgaaatagcc ttagatgaaa
     4081 acacactaaa gcttatgggt ataaaaccaa gactaggagt gactatacct atgaatttag
     4141 atatttcact tttaaatgat acaataccac catataatta cacagctaac tttaaattga
     4201 gtggtatttt agaggatgat tatacaggat atgtaagtgg tatagtaaac ggtattgtag
     4261 gcaaaggtgc atcagaaaat ctattaccta agagatatat tttatcctct ttggatttta
     4321 aaataaaaca gcaagagaag tttcaagaaa tagtaaatca attagctaaa aaaataaatc
     4381 tttctcacaa ttcaattcaa tataattgga tttacttaaa tgctcttaaa atacagtttg
     4441 aaaaagatga aaatagttct aatagtgatg gtatatctat gattatattg gtatctttat
     4501 ttgttgcctt attggtttta ctggcttctg gacttgtaat ctacaatatt ttaaaaatct
     4561 ctgttacaaa gaaaattaag gagtatggat gtttaagagc aataggagct gaaccaaacc
     4621 agatatataa gatagttatt ttacaaatct taattttatg tactattgcc atacctattg
     4681 gagctgtaat tggaattata tcttctaaag gcataactgg aatggtcact aatatcttaa
     4741 atccagatat acttctagca aatgataata aagaaattac tgagcttata cataaaaaca
     4801 caacagcata tatattccca ttagtattaa gcactagtat atctttgatt ttttcactta
     4861 tttcagcatt accatcagca atatatgctt cacatgtctc tcccaaaatt gctatggctg
     4921 gcagtactac caaaatcaaa agaaaaatta aacgagaaaa aacaataaaa aactttgaaa
     4981 gacatctagc ctggctaaac ttaaaaagaa ataaaggaag aactataata accatactat
     5041 cattatttat gagtataaca gtattcgttg cactgagtga gttctctaat gtattagatg
     5101 tatctaggtc agtaagtaat ttaaaagaag gagatttttc cttaacaaat gaaatatctg
     5161 gttttgataa gtcaatacta gataaaatag ataaaatgaa aaacgtcaat cgcacttcct
     5221 ttataaagta ctctgaatac aaacagggcg aaatagatac agatataaat tttgaaaata
     5281 gtggtgagat gttaaaaatc attggaattg atgagcaaac tttaaaagac ttaatgcctt
     5341 ctattacaga ttctatacta gaagacttta aaaatggttc tatctgtttt ataaaaaatc
     5401 cgattgctat ttctacacca ggtgtaaaaa ctagacatac aaatttaaaa cctaaagata
     5461 atatcacaat taacaaaaaa caactagata tatatagtac agtagataaa atgttctttt
     5521 tacaaggtaa tggctggata aatggtgttg atgttattgt atatgattct gtttataaca
     5581 ccttgacaaa taaaaacaag ttcaaccaga taaatattta tgctaaagat aaatcaaaat
     5641 tagaacaaat aagattgtct attgaacaga tttgtgaaaa taatcctggc tctcattgga
     5701 tatcatatat tgaatcagat aaacaactta aagagagttt taaacaaata gaatttctag
     5761 catgggcagt tatattattt gtgggattga ttggtacttt aaacatcatt aacacaactc
     5821 atacaaatat aaatactagg actaatgaga ttggagttaa aagagctatt ggtatgagta
     5881 atagtagctt atataagatg tttttatggg aaggtgttta ttatggaatt tttgctacta
     5941 tatttggttc aattgctggg tatgtcagtg ctattattat aaacatggca actatagaaa
     6001 agcttgattt tactaatata ccaattactt caattttaca agctactatt atttctatac
     6061 ttgcatgtat tatagctact cttattccgt taagaaaagt taaaaagatg aatataattg
     6121 actgtataga tgtcaattta taactatttt acagataata aataaattaa attatcaatg
     6181 actgtctcta ttttctttta aatctaacta ataaatcttt ttaactataa tataagcttt
     6241 aaaaaaggcc tgtctcaaaa tagagataag ccttttttca ttgtcgaaat aatgaaaata
     6301 tatttaactt cacaaaaaaa gagtgcctca cgaattaaaa atttcgcttt gagacactcc
     6361 cctttaattt aaaatatata gttaaatgct atgccaaaat ttcacacatg ctccacctga
     6421 ttttgaattt tctattttaa cagaacctcc aagctgttcc actaattgtt ttacaatgta
     6481 aagcccaaga ccagaatgtc ctcctttagt ctgcctagct tcatctcctc gataaaactt
     6541 atcaagagcc ttttttaaat ctttagagct aaaccctcta ccagaatcac aaatctcata
     6601 agaaatacaa tttttttcat cctttacagt aatactaata ttaccacctg atggagtgta
     6661 ttgcagactg ttggatatga tattatcaaa aattcgtgtc aacctatcca cgtcgatttg
     6721 aattggggat tgtatattcc cctgcatttt caatataagt tcaacttcct tttgatgagc
     6781 ctgtaattca tagtcttgta ccttttgcct caaaaattca ggtaaattta taggaacaag
     6841 atttaattgt atatttgact tttctaagtc agacgtatat tgcatctttt ggacaagagc
     6901 agcacttttt tctgtatttt ctcttatcac tgtaagatac ctatgaagtt tttcattgtc
     6961 atctgtatta ttaccaataa gtgcatctgt atatcccaaa ataatagaaa gaggagattt
     7021 taaatcatgt gctagagctt ccaccatttc aacacgttcc tgctccatct tccactgtgc
     7081 agataaagag ccttttaatt cgtctttcat ttcagaaaat gcactacaaa gtttaccgag
     7141 ttcattatct gaataatagt cgatttcaaa atcaaggtcc ttttctttta tcttcttgga
     7201 cgcatcaatc aatagatgta atggttgatt gatattcttt acaaagcgct tagaaagcca
     7261 tctagtaaaa cccacgatat atagaaatgg agaaaacaga gccattataa taactgcaaa
     7321 tacaaatctc cctctaatat ttgcaaaggt gattttcact tggtagaata gaataacagc
     7381 tccctcaatc tttccattat cccctttgat tggaacagta tggatataac cacctttacg
     7441 aacagtttta ttgataaaat tattaaataa ttcttctttt gttttaaata gttttttggg
     7501 gttagtacca tataaaatat tgccattatt atccacaact tgatatagca tatcatcccc
     7561 acgaatcgtt ctttttaaac cttcttcatt cgattgtgat aacaaagtta tgtttttttc
     7621 attgatgtat ttttcaattc taggaacttg tcgctcataa tagtttggtg gatagatatc
     7681 cttatttaag gaatgaatat acaaatagag agcaaataca taagtaatca ctgtggcaac
     7741 tatactggct attataataa aaataaaagt atgtctaaat tgggtaataa gggattgatt
     7801 tttcatttaa taacctctct ctctttacat tttattccac ttatacccaa taccccaaac
     7861 tgtggaaata tattcttttt ctggtgtcac agcagaaaac ttagctcgta ttttttttac
     7921 tcgttcaaca acagttgagg aatcaccttc agaatcacac ccccatactt tttcatagat
     7981 ttgttctctt gagaaaactt gtcctgcatg gagtgctagt aattcaacaa tatcatactc
     8041 tatttttgtt aaatcaatat tcttaccatc aattttaatt gcacgttcct tcatatataa
     8101 gcataacttc ccaaaataca aagtagatct cttgttctct tcattgatat actgtgaacg
     8161 cttttcccta cgcagattag cttcaatcct tgccataagt tcacgcagtc caaatggctt
     8221 tgttacataa tcatcccctc caaaggtcaa ccctttgatt ttgtcggttt ctgattgctt
     8281 tgcacttaaa aatataattg ggcataaaac atcatctcta attgcctgac atacctcaaa
     8341 tccattcatt ttaggcatca taatatccag tataataagg tcaggctcct gttttgataa
     8401 ctctatacct gcctgaccat cataagctat caacacttca tgtccttttg ccttcaattc
     8461 atcctcaagt agcattacta ggtccatttc atcatcaata atcaatattt tactcaatat
     8521 aacaccacct ttctcttatg ggaaggtcag tattaaaaca atattcgtct actaataact
     8581 tttccttccc tcccacattt taaaccaaat aacgcctcct atcaagaata taatcaaacc
     8641 tattgctact actataagct ctatactagc tgtttgcatc actttagagt aaaatgcctc
     8701 agttgaaata gttccagctt ctttaataaa ataaattcct ggaagcatac ctagttttac
     8761 tggccatgtc catggaacaa atgcccatac tttttcacca acgctagtta atccaagtat
     8821 tgctgccatc aaaattccac cgatactaat accaatagat gcacccatac cccatgcaaa
     8881 actcacccaa agatgaatag ctaatagtgg aagtgtccca atcaccacaa gtcctgctgc
     8941 tgacaggaat aaccatattg ttgctccaga tggcactgca atactcattc ctatgcaaag
     9001 tatcaacgta gcaataaatg tgcatatagt taggcaaaac agaagaaaaa gaaattttcc
     9061 cagatataat ctaacacgag aaatacctac acataaaaaa ccattaaaat ttccagcaag
     9121 ctcttcctcc tgcactataa atcctgctaa aatacctact ccaataggaa tgatgaatac
     9181 agtccatact gtaaaaaatc cttcaaaagc aaattcttgt gtacttccag atttagttgt
     9241 gagataaaaa accacacata atgaaaaaat aataggcata aaaagagtaa gccactggac
     9301 agctgtcctc tttgctcgaa gccattctga tgataatagc tttatcattt gtcatccctc
     9361 ctattgaacc aagctgcagt aagtgctgtt aatactataa acacaaccaa agaaacaaca
     9421 atcccaatag gaatcactga agtattcaaa agtgggtctc cagcttctaa tatagtaccg
     9481 ttaggatgta tgttgatgat tgggcatacc attctaactg cccaactcca aggtactgca
     9541 atccaaaatg gttttgtcgc agcaaggaca ccaaatatca tacctaaagc tccaactccc
     9601 atacttagga acataccttt ccatgtagct agccatagct gaagtggaag tatagcaaga
     9661 gaaacaaccc agcatacaat acttgcccct ataatctgtc caaaaggaac tggaccagct
     9721 ttcaatatta aaccagttat aattgttacg actattaaaa ctagcgttga aataaaactg
     9781 tagactgcca tagctataac tttattaatc caaagtgtca taggagacac attgtgtgta
     9841 cgtaaagcac gataatttcc agcttttttt tcctggaaag ctaccaaagt agcaaacaat
     9901 gcaaatccaa taggcagaaa taaaaatgac caccagttaa aaattaatgc taaaagactt
     9961 tgccacgccc aagaagttgt ttgagataat ggattttgca tgagtgttga ttgcattata
    10021 aatgcatatg ctgcaaaaga cactgggaaa aaaacaataa gtttacccat aaatgttctt
    10081 ttatacttta aaagttctga ttgtaaagca ttcatctgtt ttgtcctcct ttaagaataa
    10141 catctgtaaa aaattcttct agattttcat tagggtcagg cattccttga aataatagtc
    10201 gtcctccatt tataattcct atattatcta caacttgagc aacctctgat agaatatggc
    10261 tagaaagaat tacagttata cctttttttg gaaaagaagc aatcaattca cgcaattctt
    10321 gaatgccaaa ggggtcaaga ccattggttg gttcatctag tattaacaac tctgggtcac
    10381 tcaaaagtgc aatagctatt cctaaccttt gtttcatacc catagaaaat tgtgaagctc
    10441 tctttttacc tgtatttttc aaatttacta tttccaacac ttcataaatt ttatctttag
    10501 gtattcccaa tatttttgta tgtactaaca aattttcctc tgctgtaagg tttccataca
    10561 atgcagggga ttctattaaa gaacctattt ttgcaagaga ttctcgtttc caaggctttc
    10621 tatcaaaaat aatacgacca tttgttgggc gaagaagacc aacaagcatt ttaagtgttg
    10681 tggactttcc tgctccattt ggtcctaaaa gtccatatat actatttcgt tcaatttgta
    10741 gagaaacatt gttaactgcc atttgttctc catatctttt tgataagttt tttgtttcaa
    10801 gaattaaatc tttcatatta atttctccct tcttgtaata ccttaaatct gttactaaga
    10861 gaatactaac atgaaaaaat aacaattcta taacacctac taattttaat aaaatttaaa
    10921 ataaaaaata gagttaccaa ttttattttg gcaactctat ttaagtttat gttttacagt
    10981 tatattagat ttaagcttag tttcattatc acactaactt caaatttact ctataatcct
    11041 taaatcaaac actaatttat taatcaaaca ctaatctatt aatttatatt tctttagaca
    11101 tctaatatag tttgctacta aataataatt atttatatca tatttatcta atcctttaaa
    11161 agaagttatt tcatcattaa gctcatcact accttctaca aaaacataac ctgtttcaaa
    11221 ttccatacct acaataatct tacagccact ataaatttcc tcttgagtat cgtacaattt
    11281 ttttgcattt ccttcatttg gaattgtaaa tacaataggt tttaattctt tatcactgga
    11341 aatcttttgt gaaaaaggct tgtttaactt aatattattg ataactacat gtttaaattc
    11401 ctctgaagtt tcttctacta ttacatattt tcgctttaaa aactctatta tttctaaaac
    11461 agacttagaa tttactttta aaatactttt atatttttca tataaagatt tccactgttc
    11521 aattttttcc tgtggtactt cttggatatg tgtacttatt ttttcttgag atattttttt
    11581 caattaaact ctcctttcaa tacttaaata tccttataaa tctcacatga ctaaagttac
    11641 aaggttccta cttaatagaa ttttgcatac ttaaatatgt cttattaatt ctctacagga
    11701 tatctccgta gttcctacga ttcttacata agttacttat tttaaattgt tattagttag
    11761 attataaaaa ttatttattt tatcactaat ataatactct taaaaacttt acaaatcaat
    11821 ttaaatttgc taccttttat ttataacaac ttatagtaca tttctcctat gcttttcaat
    11881 atattacttt taaaaggagg ttttcattat gattttaaat agagctgagc ttttccgagg
    11941 atattcttta aatagattta acgagatatg caatatatta tcagtaaata aaattaaata
    12001 taagtataaa gtgaaaaata atgcttactc taaaaaacct ttttttaatg aaataacact
    12061 tggcaattta ggccaaaagg aagatttctc ttatgagtac ttaatttatg ttcataaaga
    12121 cgattatgaa caggcagagt ctctaataaa ttctaagttg cgatagatta tctataaaac
    12181 atgacattct gttgtcatgt tttatatgtt attatacttt caaatatgct actatatttt
    12241 caaaattgtg tgctttaaaa ctagcttttt ttagtaattt aaagcttaaa actaaggaga
    12301 ctcgttatat gaaaataaat ataaaatcaa agttaagtga atttattaaa cagaacaatc
    12361 tttttgatga cagcagaata atatcctatc ttcctaatat aacaatagaa acagacaaaa
    12421 taaaaactat aatgattaat gaggttgtcc caactaacac caatgacgat ttttatagtg
    12481 tagataaaga tgctgattat ttaaaaacta ctattccttt atttgacagt gcaggaataa
    12541 aagtatctaa cataaatgat atcctagata tgggtatcta tataactaat gcagtaaaac
    12601 ttcctaaatc agaatataca ataactcgtg atacaattaa attacatatg cctattttag
    12661 aagaagaaat taaattattt aaaaacctag aggtagtaat gttaatggga gatgttgcaa
    12721 aaaaatcttt taatatgatt actaaaaagc atataaaaaa gaatgtcatc ccttccattt
    12781 caacatataa acttcgtaat aatgaacttt attatgataa tttaagagta tttccatcat
    12841 atattatgac tggtggaaac atactgattg aaaaatcaaa atttgaaatg gcatcagaag
    12901 atataaaaaa aatgttcgag attataaacg gttgatatta caaaataata aaaactaata
    12961 taaaataaaa agtgcctcaa aaacttctat ttatgaggca cttttatttt atattagtgt
    13021 aactatatac tattaacata atttattatt tatattgttc cttttaatct ctattccatt
    13081 tagtctaggt aacattactc tttacaagat tactttatat ttcttttata tctctaacgt
    13141 ttttatttaa taaaacaatt attaatacaa gtcaaatata tctatttatc taaaaatata
    13201 tcttttacag taatatctgg atggtgatac ttcttcctat tcgttaaaaa agattttttt
    13261 acattaatag cctcttttgg acaccagtga aaacaagcta ggcatctttc acactcatta
    13321 ccccactttg gctttttatc aactatactt atattctcta caggacaaac ccttttacat
    13381 gtcccacatc tattacattt attttctgca aaaaatttag tagttgcaat ttgcatattc
    13441 ttacctacta aacgaagtac aacactatct atatgtgact ttaaattgtt ttttccttct
    13501 atatttccaa tctccaagtt attgatacat tttgcaatct cagctaccct attagtctga
    13561 tttttcaatc tttcttcttc aacatctgga ggtgttatca aagcatttcc aggcatattg
    13621 attaaaaaac cagcagataa ttttttcccc ctttttttaa gtaacttatc tatagtaaat
    13681 aaactatgtc cagctacccc attacaagtg gcaatggcat atatatgagt attaatatta
    13741 aaatttaatt tctctacaaa agactttact atatcaggaa catcagcaaa ataaacagga
    13801 aaaacaatta ccaatatatc ttcatttatg tctatattat tttcttttct atgtatagct
    13861 aagggtataa cttctttact tccatttaac gtttctttta aaatatttga aactactagc
    13921 gaatttcctg ttccagaaaa ataataaatc ttactattca ttcttatccc tctttctata
    13981 tttctacatc atcaatagtt aaaaagtctc ttaaaatagg atttatattt actttccaat
    14041 tctatccaaa atatcagtaa acaaaggcaa ttcttcatca gagatagatt taaatgtctc
    14101 tgttgcatat atacttgcaa tttcatttaa tggttcttct aattctctac ctaaatctgt
    14161 taggaatatt tgaaacttac gtctatcttt aacatctact ttacggacaa ttaatccctt
    14221 atttttcata acatctaaga ttcttgtcgt attagattta tccttacatg atttttcaca
    14281 taaatctatc tgagaaattc catcttgctc ccatagaaac cttaataacg cataatggtc
    14341 agttgttata tcatatccat tttgtttaaa catctttttt aaatcatctt ttacttttaa
    14401 cgaagtactt actaacttag ttgtagcttc atatagtttc tcaaaatctt tcattttacc
    14461 tccatttaaa taatcccttg ctcctcccat ctatcaatta ttcccttata ttgctcaata
    14521 tctttaaaag ccattggctt taaaacagca tttatagcaa taaaattata gatttgagaa
    14581 agcacaggtg gatttttctt ttcctctatg caataaataa attttttaga taccttttgt
    14641 atctttgatt ctactttttc agttatagga ttttgattaa atccagtttt tatagataaa
    14701 ctaccacaaa cataacctcc cgaatctaag accatggatt ttataaaaga aattgctttt
    14761 gaaccacgtg cattttcata tgtagtaact gctatacatg gcttacgata caataatctt
    14821 tcaagcatca tatgtgctct atctgtaaac accttgaaaa gtccagttac actacttcca
    14881 taagttgggc taccaaatat aacaccatca ctcttagtaa ttatatcatg aatatactct
    14941 actttatcat tttgattgat acacttgccc attttataac aatttaaaca accaatacaa
    15001 tatgatggat tgacctcact taaatcaaaa taattaattt ttacatctgg tattaatctt
    15061 tctatatctt tatacaagta agtcaaaact tttgatgtag ctccattttt tctagggctt
    15121 ccatttataa ctgtaattat catttctata tctccttttt agttgttata tcaacaataa
    15181 gtatatcaac tatattttta gctgtcaata taaaccattt atttaaagct atacttacac
    15241 agcttaaata aaagttttat ttaactacat gtgcttattt aatcaactat aaatgaatta
    15301 tttttaataa ttgacaaact aaattaatga taatatactt tatgtattac aaatgtaagt
    15361 agaaggaggt atagtatgaa aaagttacca catatttcag aagcagaata tcaagttatg
    15421 aaaattattt ggaaatatgc tcctatcagc actactgaag taattgaaaa gttggtagaa
    15481 acaagtacat ggagtcctaa aacaattcaa acaatgttat tgagattagt taaaaaagga
    15541 gctttaacat atgaaaaaaa tagcagagta ttcgtttata ctccacttgt taaagaagaa
    15601 gaatacgtag ctacagaaag tagttctttt ttaaatcgtt tttataatgg tactcttaat
    15661 tctatggtac taaacttttt agaaaatgat aaactatctg aagatgatat tgaggaactg
    15721 agagaaatct taaataaaag gacgactaag ggggataaat aaatatgtat gccccattct
    15781 ttatacgatt tttactcagt acattaacct tatcaatatt aattgttgta attttacttg
    15841 ccaaaaaagt atttaaaaaa catatttctg caaagtacca atacaatatt ggatttatat
    15901 tgcttataat gttaataatt ccatttgtcc ctcttaaata tattaatctt ggaaatgtat
    15961 ttaactattt atatactttt aatcaacttg gaagcagtac taataggact tctatgataa
    16021 aaaatagttc aaattctgta attaataact caaatgcatt aaacgatttt tcagtatccc
    16081 taaatcaagt taattttgat agtttaaata ttttattact aatatcatgg ataattggaa
    16141 ttttaatcat gatagctatt actattcatt gcaacattaa aataaaaaat atgaaaaaat
    16201 caatacaaat tttaaaagat gaggagatag tatttatatt tgaaaagtgt aaaaaagata
    16261 ttggtataaa taaggatttt atactatgca aatcacctat tataaataca cctattacat
    16321 ttggatactt taaaccacac attatattac cagataagtc tatatctaaa ttatctttaa
    16381 aagatataaa atatatctta cttcatgaat tacaacattt taaaaataaa gatatactga
    16441 taaattatat aatgtgcttt cttcaaatat tatattggtt taatcctctt gtatggtatg
    16501 catttaaaga aatgcgtata gaccgtgaaa ttgcatgtga tatttcagtt ttaaaaaagc
    16561 ttaataaaga tagtcatata gaatatggac aaactctaat aaactttgtg gataatattt
    16621 caagaccatc aaatcttaca cttacatcag atattggtgg ttcaaaaaaa caaataaaaa
    16681 agcgactatt gaagataatg gattttcata atgaatcaaa tacgttaaaa ttcaaaagca
    16741 ttttaatttt tacattgata gctttaattg tatttggaaa tattcccaaa ctatctatta
    16801 tggcagatat caacgaaaaa tatagaccta caaatgaaca aactgtgtac gaggacttaa
    16861 gtcagtattt taaaggtttt gatggaagtt ttgttatgta tgatttaaaa tccaagcaat
    16921 atcatattta caataaagaa aaaagtaaaa caagaatttc tccaaattct acttataaga
    16981 tttatagtgc cttattggga ttggaaaatg ggataataac aagaaataat tcacatatcc
    17041 cttgggatgg aaagcatcat tctaatagtt cttggaatga agaccaagac ttattttctg
    17101 ctatggaaaa ctccgtaaac tggtactttc agactctaga taaaaaaaca gggctaaaaa
    17161 acatacaaac ttatcttagg caaattggat atggcaatta tgatgtttca ggtggtatct
    17221 caaacttttg gctagaatct tcattaaaaa tatcacctat tgaacaggta gaattacttg
    17281 aaagttttta tacaaataag tttaaatttg aagataaaaa tatagaaaca gtaaaagatt
    17341 cattgttaat atcaaaaaag gatggggtat cactttttgg taaaacaggc actgccaata
    17401 taaatggaaa agatgtaaat ggttggttta taggttatgt ggaaaaaaat gaaaatactt
    17461 atttctttgc tacaaatatt caaagcaaag attattctaa tggtagttca gcatctaaga
    17521 taacattatc catattaaat gacaaaaaaa tctataatcc ttaatatatt tactgtacat
    17581 tctaaaaaat tcacataatt ttcatattaa ttatcaatta aattcatata caaggcttat
    17641 tatataaaca tagaagatag taaaactagc ttaaaaacat aatataatac cctatacaaa
    17701 tagtggcaac tctccctcac atcctcaatt gttgccacta tttaattttt tgtatgaagt
    17761 taaatatatt ttcatcattt caacaatttg ggtgcaatac aatctctatt ttgaaacaac
    17821 ctctttaatt gttttagagt ttatgaatta tttctcatac tttaaaaaga ttgaattact
    17881 cttgataatc ttcttttgta gtcattacat atcacctact actcaacaca tattctttga
    17941 cctattttta catccttgtt tatatccaaa atatttacct tcttaccatc tggaaacaat
    18001 accattgtca aagtagactt tcctaattca tgtattctct ttaaatcaac attaacaaga
    18061 acgtctccct gtttaaccat atctccttgt ttaacagcaa catcaaagcc atctccattt
    18121 aatgcaactg tatcaattcc aacatgaatt aatacttctc cattagttcc tttaatccca
    18181 aatgcatgtc cacttgaaac cactgtttct aatttacctg aaataggaga aacaattttt
    18241 ccatctataa tatcaatagc aaaaccatca cctaacattt ttttagaaaa tgtttcatct
    18301 ggtacatttt ctaatgcaac aattttcccc ttagctggag acacaaagtt ttcattattt
    18361 tcattaaaca ctaattcttc gtcttcatct tcatttatag cttctttagg cactccaaat
    18421 aaacatgtta caataaagca gaatgcaacc acaaatattg atactaataa gtatgtgatt
    18481 aaattatatc catcatagat atacattaaa taagaaggta aaactgctaa tccatatgaa
    18541 ttagattgaa tatgtaaaat tgataatatt gctgctccta aacctgaaga tgcacacata
    18601 ataatcaaag gctttaaatt atatcgaagc tgaatcccaa ataaaacagg ctcacttata
    18661 ccaaaaagtt gagatagcat tgctccaacc gcagtttgtt taacttgatt atttttagat
    18721 tttttcataa atgctagaca tgttccaata ttcgcaaatc catacattgc acaaagagta
    18781 ataagggcat taaatcctgt atttgctaat agtgatgttt caatcattac ataagtatga
    18841 tgtaagccag taataacagc taatggatat gtaaatccaa taataaatcc tccaattcct
    18901 aaaggcaaat caatcaataa gctgatgata tcaaccattt ttaattctat tgtatgcatt
    18961 atagggccta cacctaacaa aataactaac attgttatta gcataacaaa aaatggtgtg
    19021 aaaataaggt ctaatacatt aggcatatgt ttacggaacc atttttcaag attagctcca
    19081 ataaatgctg taacaatagc tgttaaaaca cttccttgac aacctacaat aggaataaat
    19141 ccaaatgcca taattgcttc aatttcattt gatgggtcag caacagaata tgcattagga
    19201 agtattgggg aaactaacat aagtccaata acaagtccaa taacaggtgt tcctccaaat
    19261 actttaaatg ctgaccatac aataattgct ggtaaaaaag caaatgctgt ttctgtaaga
    19321 accgaaacaa gtgtctgaat atacaatgga atatttgcag aagacattcc aaataatcca
    19381 agtacattat cattaaacaa acaacccttt aatcccaaga ataatcctgt tgctgcaata
    19441 actggaataa taggaacaaa tatatctgct agagttctca taactttctt aaatccagtt
    19501 tcattatttt taactaattc atcttgttct ttttttgata aagtttttaa tcccattttt
    19561 tcaacttctg cataaacttt attaactatt cctgttccta aaataatttg atactgtcca
    19621 gaagtaaaga aaaccccttt aacctcatca actttttcaa ctttcttatc attaataatt
    19681 tctctatctt taaccatcac tcttaatctt gtggcacaat gtgtaataga aataatatta
    19741 tcttcaccaa taattttaat aagctcattt gctattttat tatatttatt catttttcta
    19801 cctcactttc aataatactg catgaaatgg tttcaaacgc actgttttat ttaatagatt
    19861 tatatcatct aaattttgga atacaatatc atatttttct tctactctta tttctacagg
    19921 tttatcagaa aaattataat aacaatcaat aactaccaaa tctaattgcc ttctatacgc
    19981 aataatatca tcactttcta tatcaatagg ttctattgaa ccataaatca aacaatcaga
    20041 atactcacta tcttgtctca actttatcat ctttttataa aattctaaaa tactctctga
    20101 attgttaatc tgactctgtg cattaatatc tttataattt tcaataacct ttaaccatgg
    20161 tgttgtatct gaaaaacctg cattttcttt atctgcccat ggaaatggcg ttcttgcatt
    20221 atctcggctt cttaaattca caaattctaa cgcttcttct tctgagtaac tttcatctat
    20281 tgaacgatag tattggtcaa tgctagataa atcattaaaa tcatttatgg tatctctttt
    20341 aaaatttgtc attcctaatt cctgtccctg ataaataaat ggggttcctc ttaaaaagaa
    20401 atacattgct cccaacattt ttactgcatc atcattcctt tcgtattgca aaagatactt
    20461 tgttgtagct cttggttggt catgattttc aataaaatta gctgcccatc catgttcctg
    20521 aacatacatt tgactatcta aaatgcattt tcttaaatct ggaattgtcc atgaaattct
    20581 tttaaaccac tcactaccag aagcaacatc taagtcagca tgtttaaagt caaagaccat
    20641 tgaaaagaaa ccatcttctc caataaaatc acctagctgg tcatagctaa tacctgctgc
    20701 ttcagcaaca gtcatacaat catatttttt aaacgtattt tcttttaatt cataaagcat
    20761 tttttctatt ccaggctgat ttctaactgt ttttgtacat ttaacaagac catcaacacc
    20821 atcactttct aaactttgaa aacttaaatt ctttttgatg aatgtaatag catcaactct
    20881 aaatcctgcc actcccattt gagaccaggt attaatcatt tggtaaagtt cacttcttaa
    20941 tattggattt tcccaattaa ggtcaggttg ttttttacca aaagaatgaa aataatattc
    21001 gtctctattc tctacctttt cccaaacact tccaccaaaa acagacctcc agttatttgg
    21061 taaatcgttt ccttttttaa aaatataata atcatgataa ggactttctg gattttttaa
    21121 tgcttcttga aaccaaggat gttcatcact tgtatgatta agaactaaat ctaatataat
    21181 tttgattcct ctatctttac tttctttaat taactctttt aaatcttcca tacttccaaa
    21241 ttcacttgca atatccaaat aatcactaat atcataccca ttatcgtcca taggtgattt
    21301 aaaaattgga cataaccaca atgatgtaat acctaaattt tttaaataat ctaaacgaga
    21361 aataattccc tgtaaatctc caattccatc attattacta tcttgaaagc ttcttggata
    21421 aatttgataa acgatttctt tttgccacca tttcttttcc ataaattact cctttttata
    21481 tgatatttct tctttttaat tctttataaa ttccattatc gaatatgtcc tcacatatag
    21541 atgtcgctat atctttaagt tgttgatgac tatttttcat tgcaattgta acgtctgatg
    21601 cttgaaacat gacaacatcg ttttgaccat caccaaaaca tatggtttct ttttgagtta
    21661 ctcctaatct ttcttgtaat ctcttaattg ctttgccctt atgaaaatct ttttgaataa
    21721 tttcataata ctgactagaa atatctctct gtgctaattc cattttatct tgtagtatat
    21781 ttttcacttc gtcaaatacc ttttcatttg accataaaca aattttatgt atatcttgtg
    21841 acttatactc ttcaatgtta ttttcatatg ttattttttc ttggatatgt tgtttattta
    21901 tacaagaatt agttcctttc aactgattca ttgtttcaaa tatttctttg gctttttgat
    21961 tcatgaaaac tttttcctga ctctcaatcg aaaaagcaac ttctctcttt tttaataaac
    22021 aaacaacttc ctttatcaat ctttgattaa aagattgatt ataaagtaat tcaccatgat
    22081 attgaatgta attacctcct cctgcaatat atccatcaac tcctaatgat aaaacatcat
    22141 cttgtattgt acccatactt cttccagtac aaataacaac actgcaatga ttcttttgac
    22201 ataaacgtat agcatgcttt gcagattctg ggattccata tacctcatct ctaagcgtac
    22261 catctatatc aagtattaat aatttgtaca atttcatctt cctttcttac tatcaaaatc
    22321 ataatagcat tcaaaaaaaa agatatcaat attttgatat cttttaaaaa atatgttcat
    22381 ttttagcaca aattttcatt ttcggggaat atgttcactt tctaaataca tcttgtattt
    22441 aatataaagc agttcaatta aaataaaata aggtgttgtt acatcgtagt ccagttgttt
    22501 atctacagaa atagatatag attgaatata caggttttca tcacataatc ttgctaaagg
    22561 attacttatc atttttgtaa tagaaaccat ataaattcct ttcattctca tttctttagc
    22621 aaaatcaata attgtagatg catctccatt caatgaaata aagaaaatca tatcatctgt
    22681 ttttataaaa tgattaagtg cctgaatcat atcataacca taaacatcat atataatttt
    22741 ccctgttggt aagaaaatcc ttttcatctc tttagcaact ctttcctgag aataaccttc
    22801 tccaaagacg attatacgat ttgccttttt cattctttca aacataatcg aacaatcctt
    22861 cttctctata tcatctatca ccttattata acaatccatt aattggtgtt tactttgatt
    22921 tttatattct acttctgttt catttaagcg tataataact cttaattcac tatatccagg
    22981 taattgtagt ttttgagcaa aacgagaaag tgcacttgta gatatatggt atttgtctgc
    23041 aaattcatct atagaaagtt taatacaatc ttctttatga ttaagaatac actcacagat
    23101 atacttatca tttgctgtaa atgaattata atatttattg aaaagtcctt caattttcat
    23161 tcttttctcc ttctatactc tatgtaattc atatgaagca aatctaatag agcataaaat
    23221 gctcctacga cttcataaga tatagcttgt aatgtatcaa gtttttgcgt ttcaacatac
    23281 agattatact cacaaagtgt tgcaattgta ttattatcaa gcctagttaa agaaatcgta
    23341 tgtatatgat tgttgataaa ttttgcaatt tcaattcctg cttttgtttc tccagacaaa
    23401 gaaatgataa taaataaatc atttttttga aatgattctc tcataaattc aatttcacct
    23461 atatcaaata agtcaataac acatttacct actgaaagaa atattctctt tatttcttga
    23521 gctaaacttt tctgttcatt acctgtccca taaatataaa ttgtttctgc atcatatatt
    23581 attttacaaa tatcttgaaa tgatattttt tgtaattcat tcattaatag tcgataattc
    23641 tcgcaaacat catgaaaatt attatcatta ttaacaaatt ctagttgatt atttttaagt
    23701 aaatacttta aatcagagaa tgaatctaat ccaatttttc tacaaagcct taataaagta
    23761 gctctagaaa cattacattc atttgcaatt ttttcacacg aaaaattacg gtattctctt
    23821 tgacaaatca atatttgttt aataataatt ttatcatttt catttaaatt atcataattt
    23881 tggttaacta acgattctaa tctcattttt tccactccct aaatattatt ttaatataca
    23941 ttagttgctt tttctaggga agagttttaa ataataagga taaaataatc atttgatttt
    24001 atttttatta aaataaaaat aacctgctaa aagcaggcac caaaaagtac ttatcctttc
    24061 gaacatgtac tttagatatt gctttcttcg gaagcttcat ctcttcatac tctaaaatat
    24121 agttttttct aactcaaaag tattttccat ggtagaaatt actttaattc atctgccatt
    24181 tactttttat cttatttaat aattactatt acttgaatat ttacttcttt aagtaaattt
    24241 catagttaac aaagctaaaa ctattattat agatgttaaa atacaaaatt gtaagtattg
    24301 ttataaatat gcttacagct acaaatatac atgatatatt aaaaagaagc tgtaaattat
    24361 tatcatacat acaagcaact atgcctctat taatccatgt taatttttat tatttaatac
    24421 aaggagttcc atgagacata catatccatt catattttaa attatccaat tctcttatag
    24481 atttcaccat ctttttataa tcccaattcc atggattagg ataaggaact atatttccat
    24541 tcttattttt gactaaatca cctgcaaata atacagcttc aaaaatcatg cagacatgcc
    24601 ctggagtatg accaggagta tggataattt ttatattccc aatctgcata ttctctccat
    24661 aaggttcaac atccttaatg agccttctgg aaattgcctt tccaataaac ttcttaaacc
    24721 caggtctatc cttctctcct ataataaaag gtatatcctc tacatgagcc catacttttg
    24781 ctcctgtttt ttctttcaat aatttaatat tagatatatg gtcaacatca tgatgagtta
    24841 ataaaatatg cttaatatct gtaagtttaa tgccaagtgt ttttaattct ttaattatat
    24901 ttttccccat aaaaggaaga cctgtgtcta taagaataat ttctttatca aacacaataa
    24961 aacaatttga gccacgagta ctatctaaca ttaatacatt ttcttttatc ttcataaata
    25021 aattcccctt atttggtatt ttacctatat aattaattaa taaagttata aatagttata
    25081 atacttgata ttaattttat tttttattaa agtatactat aggtatacaa gtattatatt
    25141 tacataagta taccatcagt atactttttt atcaatagaa aggaataatt tatatgtcag
    25201 aattatcaaa aagagataaa gaaaaaatac aaagagaaaa tgagattatt gataaagcag
    25261 aaaaattatt ttgtcttaat ggatttgata atacaactat gaatgaatta gcaaaagaag
    25321 ttgaatatac taaacgcacc atatacaagt acttttcatg taaagaagat ttattctttg
    25381 ctgttgtctt aagaggatat aaaagacttt gggataatgt taaaatagaa agtgcaaaag
    25441 gcaaaacagg gtttgaaaaa ataaaacttt catactttgc ttttcataaa ttttactctg
    25501 tagaaccttc tctattatgc cttatgggaa tgattggtat tgtaaactct gaaaaatcgg
    25561 atactgatat gctgtttaaa gaaaagtttt tttcttttaa taaatttatg tttgatgaaa
    25621 tacaaggaat gtttgaaatt ggaaaaaatg atagaagtat taggcatgat attgaaatac
    25681 ctactcttat gtattcatca atatttacac ttacaggatt ttttaattta ctatctgtaa
    25741 caggaaaatc atacctgaac aactttaata tagatgagga aaaatttatt gaaacaactc
    25801 ttgctctttt aatagattca attaaagcat aaacctaagt ttataaatta aagtttatat
    25861 acttaaatga taattttttc tatctctaat ataaaaaata ttaaacataa aactaataac
    25921 ttagttaatg gaataactaa attactgaaa ggattaaaag taaatggcta aaatacttct
    25981 tttagaagat gatgatacaa ttacatttgg aataaaaact tcccttgaaa agaaaaatca
    26041 cactgtatgt tgttataata cgattgctga agctaaaaaa aattttgata aaactattga
    26101 tttaattctg cttgatttaa atctacctga tggtattgga tacgagtttt gccaatatgt
    26161 taaaagtata gaagatactc cagtgatatt tttgacagtc cgtgatgatg aaaaagacat
    26221 tatacgtgga ttagatatgg gtgcagatga ctatgtggtt aaaccgtttt tgctctcagt
    26281 acttcagtcc agaattaatg cagttcttag aagaactcat tcaaatacta acaatactat
    26341 tacatgtgaa aatataagct tgaataaggt tgaagtcaaa gcatatttag ataatgttga
    26401 agtacctttg acagcaggag aatataaact tcttctaagt cttatggaaa ataaaaatag
    26461 agctatgact cgtacaaagt tactagaaat gctctgggat gtagatggta atttcgtaaa
    26521 tgataacact cttactgtta caataaaacg tcttagagaa aaattaaaaa atccaccttg
    26581 tataaaaacg ttaagaggca taggatatag aatggaggac agctagtgaa aactccaaga
    26641 aatatcaaca gtacagttgt ttttataata ttctgcttaa ttgtaacttt tttattgagt
    26701 gtaattctaa caactataac ttcttttaat gaatacaatg tactgtctaa ggctcttggt
    26761 gcatcatatg agtacaatcc taagtctaca gaaagtatac taaaatcact gaaaaattct
    26821 agtgataagg attttaaaac aggaaatgaa attttatcaa aacatggata ttctaaaagt
    26881 acattttgga gtaaaaactt tttttatttt ctagtgatat cgtttggtat aatttcaaca
    26941 atggctacag ttatatatat aatcaaacat agaacaaata agcaaaaact gagtagaata
    27001 aacaatctta ctcaatactt agaagaagtg aatttaggaa aagagtctgt acttttacga
    27061 tgtgaagatg aattttctca ccttgaagat gagatatata aaacagttgg ggaacttcgt
    27121 tgttcaaaag aatttgcttt aaaagaaaga caaactcttt cagataatct tgcagatatt
    27181 gctcatcaac ttaagactcc aattacttct atgtcactta tggcacaatt actttcagaa
    27241 aattgtccag atgaagaaat agattatatt aacagactta caaatcaaat ctcaagactt
    27301 gagagactag tttcatcttt acttacatta tcaaaattgg atgcaagtac tttaatattt
    27361 gaaagcaaac ttatagatgt acattctctt cttacttatg caattgagcc tatagaaggt
    27421 tctttaagag aaaaacatca gacatttact atcgaggctt ctccttcggt agaatttgaa
    27481 atcgatatta attggacttt agaagctctt ttaaatattc ttaaaaattg tagtgagcat
    27541 atagaagatg gtggatatat agttgcttat tatagtcaaa atcctctata tgttgagatt
    27601 atcatagaag ataatggtaa ggggtttgct caggaagaac ttccatatat ttttaataga
    27661 ttttatcgtg gtaaaaatgc tagtaaggac agtattggta tagggcttgc tctatcaaag
    27721 tctattatag aacgtcaaaa tggtactcta catgcagaaa attgccataa tggtggtgca
    27781 agattcataa ttaaatttta cacataaaat gctggattat aggtaaaata cctataatcc
    27841 agctatatct atattctatg ttatctattg ttttagtaat atacttctat ttacatttaa
    27901 ggttttatta aacaaacaac ttaactatcc ctatacttgt aagtagtctt aaatcgaaac
    27961 ctttaaataa gttcaaatag attttgttta tattgtttca ttttttatag attctactat
    28021 attttcattc ttcaactgct tgtatcctgt catatatgat gctattacaa taaaaagtat
    28081 caatatagta aaacctatta ttgtagatat aggtgcaaat ggtaaatact ctataaaata
    28141 tatttcattt atacgtagaa aagcatacac tattcctatt tgcacaggaa tactaagtag
    28201 taatggtgta attcctaaga ataaaccttc caaaataagc atctttttta tgccttctgg
    28261 agataatcca acagaacgca acatagcaaa ctcttgtcgt ctacttctaa tatttcctga
    28321 cactgtagca agtacatttg ataatccaat catagccaat aaacctgtaa taaagcctac
    28381 tattaaattc atggtcttta ttccactagc cttttgtttt tcactttcta aaacatcctc
    28441 aatactataa tctcctgaac catagtcttt attataaatg gactctaatt tttctcttac
    28501 tctaggaatg tcctcacgat tatttacttt aaatactcct atcagcctat ttgaacgtaa
    28561 cctacttact tcatcataat ttttagatat ttcctgtacc atagacattg gaactatttg
    28621 cattaatgta tactttgaac tagtatctac tattttagga agtgtgcttg taatagctcc
    28681 cactttcatg ttgtaagtat aatccccttt atcttcgtca tatacttttt catttaattt
    28741 aatgttatct cctattgaaa gatttaaata atctatatat actttatttc tacgtgtact
    28801 acgattaata tcttcctcct tattatatac aattgaggtt ggattttttg tattataaaa
    28861 taactttggg tctacagaaa tttgcctaca atattctgta aaactcttat catcaagccc
    28921 aattatattt gaaattatac gaaacttgcc atcacgttct ataggagagt attttttaga
    28981 tgaaactata cttttaaatc ctccattttt cgcaagttct tttgatgcat ctttttcagt
    29041 cagccaagtg gaagcagaca ttttttgata atatagtgaa ctttttacac cttctgtatt
    29101 atctaatttt tcaatgaaag cagactctat aggttcacta ttttccaaat aaactgctat
    29161 atctctttgg tccttataat tatcacttcc ataaacagct tttgctcctt cttgatttgc
    29221 aattatatgc aaaaaacatg taagtaataa aaaagataat gttaaagata ttgttgcagt
    29281 acgataagat tttttacgag cacgtagtgc gtttgatgca agttctcctt ctattccaaa
    29341 tatctttcct aatgaatatt tatgcgactt tttgatttta acactatctc cttgtcgtat
    29401 tgcttctata ggagatatct ttgatacttt tcgtgctggt ataagtgcag aaatccagac
    29461 tgttactatt gtaagtaaaa ctgctggcag aaaagcagga ataccataag taaataccac
    29521 ttctggaaca tcagtatgat aattaactga attaacataa actataagcc tattacacaa
    29581 taaccatcct aaaaataaac ctattggtaa tggtataatt gtcagtaaaa agccttcaaa
    29641 cactacagac cttttaattt gttttggtga tgcaccaaca ctagataata tccctaactg
    29701 agtaagtcta gcatttgcag acagtgaaaa cgcgttgtaa atcaccatta caaacacggc
    29761 tactgtaaaa attgctattg taagatacat aataggagtt gaaaattttg atatactact
    29821 catagatgct ttctgttctg gtgaaaagac taacatcttt aaaagataat tactattgta
    29881 tttaagattg tattttccat attcattagt cttccatccc aatgatttag caagttttgg
    29941 aagttcctta tacgtgtctc ttatattttt aaatcgcaag tacacagtta tactatcatt
    30001 aggttttata cttgttttat caagatatcc aagagctgta tatgcaggta ttactgatga
    30061 agtagaaaca tctacctttc ctactattgt caatgtagcc tctgaaattt ttttaaaact
    30121 ttcccctttc gattttgggg ctatgggttc aagaggcttt ccatttaaga ttctatatcc
    30181 tattgggaga gtaattttat caccaatttt tacacttgga ttattctcaa aatattgttt
    30241 tgataatgca atttcattag cattatttgg tcgacgaccc tctattatag tatccttttc
    30301 tggcatagaa ttccaatact ctgatgtagc atttctataa attatataat ctcttttctc
    30361 atcattaatt ttgcctacat accattgtcc ttttaccatt acatcatcaa ctgaagaaaa
    30421 attttcaatt aatggtaagt cacttccata agtatagtca aacaattcac catgccagtt
    30481 accttctttc tcaattgaaa gtcttataaa atctgtccac atggtataca acagtccaca
    30541 caaagaagac atcatggttg tagtaagtaa tatagctaac attatagcta tactactacg
    30601 tttattgcgt cgtacaaagt ctaaggtgta ttcttgaaga atattcattt cttcaccacc
    30661 ttttcagaga caatcactcc atcttccata gttacaatac gatttgcctc tagtgctatt
    30721 ttctcatcat gagtaataag aagtattgtc tgattaaatc gtttatttga taagtttaac
    30781 aagtctacaa tttcttctga attttttcta tcaagattac cagttggctc atctgctaat
    30841 aaaatagctg gtctatagat taaacttcta gctatagata ccctttgttg ttgtccacct
    30901 gaaagttgac ttggaagatg gtttaatcta tcagataatc ccaatataga tacaatctca
    30961 gagaatctat cctcatccac ctttctttta tcaagtaaca tgggtaataa tatatttttt
    31021 ctaacatcaa gggtaggtat aagattataa aattgataaa taagacctac ttttctacgt
    31081 ctaaaaactg caagagcttc ttcctttaaa cttgatattt ctgtatcttc aacataaatc
    31141 tttccagatg taggtctatc tactccccca agcagatgta acaatgtaga tttgccagaa
    31201 ccagatgctc ctacaatgga tacaaaatca cctttttgaa ctgataaatt tacattatta
    31261 agggctgtca ctctggtttg atttgaacca tatatttttg tgagattttc acattttaat
    31321 atttccatat acaatcatct cctttatggt taaattttag caacctaaaa tgacattata
    31381 gtgacatgaa tacaaatata aattactatt aaaaatattg tttagttttt gcacattatt
    31441 taaaattata atataaaaca aaaactatgc aatatatttt atttatactt ttaatatatt
    31501 acatagtctt taaaaggtat taactttaaa tctataggta ttcaatttaa taatcaatag
    31561 caagtatttt atgtactaaa ggagttttgc tctatataga ctcaatttag taaaattatt
    31621 taataaatat tacagactat aggtaaataa tatttacaaa taaaattaag tttattccct
    31681 tacactataa tatttcttaa ttatattaat tgcaatttcc tttacctttg gccctgtaat
    31741 ttctttattg gaatcatctg atttaatagc aaaataatag gtgtcacctt cacgctctag
    31801 cataccaaca aaccatgcat ccacacgttt atttttttca tcaaatcctg ttccagtttt
    31861 tccatataca tttatgtttt tatcatttat atctattttc ataatatctc ttaaaatatt
    31921 aatatgctct tttttaaagt ttgtatctcc ctcaaatatt tttgccattg tttgcacttg
    31981 ttcttttgga gatatttgta atgaagattc taaccaaaat ccatttaaat gtccctttcc
    32041 attttttaaa tcgccttccc attcgctaat atcgcaattc ccatacttta aatcatctaa
    32101 aatgttttgg acagatttag catcaacttt atctatcaat tttttgtaat accaaacaca
    32161 agactcctta aatgcctctt ctaaactaag atttttattc caatttttat ttggatactc
    32221 tgtaccatca tatcccataa ctgattcttt tgagtttatt accccttttt ctaaaccaat
    32281 taaagttgaa actattttaa aagtagaaca tggtgacctt cttgtttcaa ttaattcttt
    32341 gttatatata ttatattctt tatttttagt attataaaaa atagctcctc cactaatacc
    32401 ttcaaaacaa tcactatagt caaccatatt aattttttct tttttgtttt tatcattgtt
    32461 ttcaattttg gatttattta attcagactt atttaattca gattttatat aattcttatc
    32521 agttttttga tttatatcgt tatgtttttt ccctacataa tacattacca taactacacc
    32581 aaccaataag atagctatcc aaataaaatt cttttttctt ttcataattt atatcatctc
    32641 ccatatttaa atttataacc tactattaaa atagtagaca ttatttgtaa tatactccaa
    32701 tctaggttct acttataaga aacaagacta aatgaatagc tgtatctctt atattttctt
    32761 cacctagtac aattatgttg attaaaccta ttgcgaagat aattaggtaa ctattaataa
    32821 catcctgtcc tccttttaat ttgtgatata ataattgatt ccacatttaa aacttactat
    32881 tgtaatattt aaatattaca atagtaagtt ttaaatgtca agttagttta ggaaagttat
    32941 tttaattgta taattgttat tacatttata aaatatggca tgtttaaaat tattttgcta
    33001 ttgggaggat taagataagc agtattataa aagctataaa ttataaaatt taatatataa
    33061 tttatagctt ttacttttac aaatattgtt tttattgatt tattaatgaa ttttattgac
    33121 aggttatatt tatataagta aacacttaca taataagtat ataatctaca tatctataag
    33181 ttgagtcaat tcacaagtat atgtcaactt aagtttatgc atagctcgtg tacaggcaat
    33241 atataacaaa ctgcggtcat aatcactata atatgtttta ttatttactg aaggtacaat
    33301 aacttcatca aactctaaac cttttgacat tttaatagaa gttatggata ctccttttgt
    33361 aaagctagaa ctttcagaag atattaagtg aacactatat tcttctctta acgcatcata
    33421 tacagcttta gcgtcattat ctgtctttaa tataatacca agagtcgcat tatcactctt
    33481 tttaaattct tcaatttcta tttttatctt atttatttca tcttgctcgt tattacattt
    33541 taataaagct ggttcttcac catgtcgttc aataggctct aatgaagata tgtcttgaat
    33601 ctttttggcg aaattaataa tctcaaaagt agaacggtaa ctcttgttca acataactaa
    33661 ttccccatca ttatatagct gtttcatatc atctagtgta tgagtatgat taggattaac
    33721 caattgtcca aaatctccaa gaatagtctt tttacactta aaaagtaagt ttattacagc
    33781 atattgaata ggtgtatagt cctgcatttc atcaataaca atatgcctta taatcttatc
    33841 ttcttgtata ccttcatatg cagcatgaat atatataaat gggtatacat ctgaccattc
    33901 aagtgttttc ttttctgcca tcacaaacat gtttgatata ttcatttgtt taaagaaatc
    33961 cttatatagt gtaagagtat tttttattgt caacattcca ttcaaacttt taagtatact
    34021 ttttaccttt ggtaaatctt cttccataat attatctgat tcaaatttat aatgaatgtc
    34081 ttcagctacc ttctctaatc tcttcttaac tggatatctg ttatatgcct caaatctact
    34141 ctgtatccag tcacttttag ctgtaaaact tccaaaagtg tagtcttttg gaataaaaat
    34201 cttatttggc atttgtttta tataatcatc aataagtttt acaaaatcaa gtgtagattt
    34261 aaaacgcact ctttcagacc atttagcatc atttatttct aatgggtctt tctcactttc
    34321 aaaattaata actctatcta attgtacctc tgctatattt tcaaaactta attcacataa
    34381 tggctcttct ccaagctcag gaaggacatt ggaaatatag tctccaaaga ctttattagg
    34441 tgataggata attacattat ttgctgatat tttatcttta aatcgataaa gaagaaaagc
    34501 aattctgtgt aatgctatag atgttttccc agaaccagct accccttgaa taatcagtgt
    34561 atcagcttta tcatttctaa taatttgatt ttgttccttt tgtattgttg aaatgattga
    34621 cttcattttt tcatctgaag tgtgactaag ttctttttga agaatatcat cttgtatatt
    34681 aatggaactt tctagtgcat attctaattt tccattttta attttaaatt gtcgttttct
    34741 tgttatttct ccatccaccc atcctattgg agcatcatat ccagcttttc cgatttcata
    34801 atcataaaac atactggcaa ttggagaacg ccagtcaagt attataagct catcttcata
    34861 atcaaatgca aatctaccaa tataatactt agattcatca tccatatctt tcaacctaaa
    34921 atctattcta gcaaagtatg gagaatcttt gattttatta atcttatctc gaacttttac
    34981 cataaaagcc ccatagttat ctatttgttt tagtccttgt tcattttgaa acatttcatg
    35041 tgggtctatt tcaccacgat tttttaccat atacatttta gcatccatat aatccttatc
    35101 aagcttgtca acaagatttt cagctttctt aaaagcattg tctagcttgt tatttatctc
    35161 tattagatgt gcaatctcat ctggaaaaaa agttaataac tctgcatcat attcacacat
    35221 attatgttta gaattagatg caataatatc gcaatttact acttgtttat tttcagacat
    35281 ttaaatcctc ctttgttaat taagtactta cactgaatca ttatacttaa ttgtgttttg
    35341 gagtttttaa tcattcttgc tatttaactt aaaggaagaa taaatttttg agtaggatat
    35401 ccaaattcca caagcaattc tgcttctgca aagcccaact tctttatcat atctctatgt
    35461 cctgtatcag ctttgtcacc ctctcgaaag gtagtaattg aaatatctgt atctatcaag
    35521 aaatcttcaa taactttctt caaaaacact tgtgcaatgt ctgaattacg atactgtgga
    35581 tgtattccaa aaaaatcaat acttctagtt tctctattaa acaccatatt tgcaatagcg
    35641 acattattaa gttttaatat taaagctctt tcttctaaaa tatactgttc taattgatat
    35701 atatattctt cctcttgcaa attgggaaat ccatctatga ctaatctcac taaattcatc
    35761 cataatggta tgtcagacat acttgcaagt ttaatttctt tttccaactc tagtacattc
    35821 tttgaaaata ggttatcttt tttcaaaaca aatcttagct gcaaaggata aaattcttgt
    35881 tctttcctat atttatttgg agatttttta tacatagctt taaatataga agtgaatgcc
    35941 tgttgacttt catatccagc tatcaatgca atttcaataa ttggtttctt tgaaaatacc
    36001 aataattttg ctgcttctgt caactttctc cgctttatgt aatcatgcat tgttaggcca
    36061 acagaagttg tgaaagttct atgcaggtga tattttgagt aatgaacagc attagcaact
    36121 atatctaaat ccagcttttc tgataaatgc tcctctatat agtcaatagc agttatgaca
    36181 ttaattactt tgtaagacat tgcagcttcc ctccttttat ccatgcaaca ttatatcaat
    36241 tattacttag ttatatttaa tgaatcttgc tattttataa acaaatttgt tgtttaagtt
    36301 ttcaattcat aaatttaata tattactttt tactttctga ttaaagactc taatccttta
    36361 attttcaata acttttcatt tagtaaatct aaaaagttat ttattatttt cttcttatta
    36421 gattccttaa ttaaatgata aatatttata ttatacgact aaaaacagct ttttacaatt
    36481 atttaatatg attacaaact taccctatct taaagtttaa tggggtcctg ttatgaatct
    36541 ttttatttta atttaagctc ttcatcagtt tattttatat attgatgtat ttgatttcat
    36601 acaaaaaaga gtgcttcatg aactaaaaag ttcattttta aacactctct tttatgtaaa
    36661 taaatttcta ttctattttg aaatattata atttgactta taatctatta ataccaaaaa
    36721 taataaattg tacgtctaag aatagattct aattagttgc tcagtagtaa aatctgtttt
    36781 taactaatcc tgctttgtat gttgaacata ttctataatt gtaccatctg gatgtctcaa
    36841 tgtcatatta tatcctgtag gaactttttt cttatctctt actacaactg ctccattatc
    36901 caatagataa cttctccatt catcaacaga atcaaccata aaagtagatt ttgtttctat
    36961 aaatggttta agagcatcat cagaaccaga taatagtaaa acatttccta tttgcgctaa
    37021 ttccaaccca acttctttat aggaaaaacg caaagaacat ttttctttta aaagattctc
    37081 ataaaaaggt atggcttttt ctatatcttg aatataaaaa cgtgttaata tatcatatat
    37141 tttcatattt tccccctata tatctatata atattacctc taaattatac cactctacaa
    37201 attatataac aataaattaa gcatagcctt ttgagcatct taagtagacc ttaacaatat
    37261 attggggtta cattagaata cataaattaa atcaccttga tattatttag gataaaattt
    37321 atgtagtagc agtaaatgtt cttctaatta tcaactaaca aacgataatc tttaactttc
    37381 tatataatgg gtaacatcat tttcgtaaga tattcactag ctggacgatt gatatcattt
    37441 aaatactggt aatatatatc gccatgaagt ttatatccat tgctatttgc ccatgaaaat
    37501 aagtcttcta aagtagggtc ttgtaattca taaagtccta aatcaattgc agttattatt
    37561 ttctgtgcag gaacaatctt ttgtagaatc tcatttttcc catctatata agtggctaca
    37621 ggaaacccta cttcaacatc caattttact aaatccatgt tatgaaaaca tacaatcggt
    37681 gctcctgaag ataaaattcc tctctcctcc agatatttag aaattttttc aaaactttgt
    37741 tttgaaaact ctgaaaattc taccataaag tcaattgttt ttcgtattgc caaaaaacag
    37801 tactcagatt gttcagctat tgttatattg gatattctag tcattttatt cctccattac
    37861 tttattttca tttctaatct ttttatacca aattatacga aaattcactg tgtaactaat
    37921 tatttcatat gaatataaaa cataacctcc ttccatttct tatatcgaaa gtatagcaaa
    37981 aataatctca tcattcttgt cacacagtgc tcttataatc agaacatact taaaagactt
    38041 gcctaatttt taggcaagtc taaaagaaat ctttttctat aaaaaattta tacaaacaat
    38101 taaaatatca aaatatagac taatataaaa ctataaattt actaatctga acctcccatg
    38161 gctaaagtca caagattact gatttataga atttcgcaca ctaaaatatg tcttactaat
    38221 tccccacagg ctacccccct agttcctacg ttcttacata agttatctat tatcaaacat
    38281 caaaatttaa acaagtttca ggttcatctt ctattcttgc tattttaaac ttacatgcaa
    38341 ttaatactaa agatactatt ggatttataa tatttaataa agcgtatgga ccatatgtta
    38401 gtgcagatac acctaatgtt gctgttatat acgccccgca tgtattccat ggtactaatg
    38461 gagaagttaa agttccacta tcttctaaag ctctcgacaa catcttagga tgtatacctc
    38521 tctttttata ttccttatta tacattcttc ctggtataac tatagataga tattgttcac
    38581 ctgctattat attggtaatt gtacaagtta ccatagtaca taatactgtt ccaaatacac
    38641 cctttgctaa tcttagtaaa gaagatgcta ctacttcaag catacctgtt ttttctaaaa
    38701 tacctccaag agataaagcg catattgtta aggatacagt ggacatcata ttcataatcc
    38761 cacctttaga taataaagca tctacctcct gtatttgtgt tgacgaaaca tatccatttt
    38821 gtgctgcatc taatatcgta ttcatagatt ctccttgaaa aactactgca aacaatactc
    38881 ccaatacagc acctactata agccctggta tagcaggaac tttaaataca accaatccaa
    38941 ttactattgc tggagctaat aataatactg gtgataaagt attaaatgta gaatctaatg
    39001 tagacctaat taattctatt tgttttatat ctagtgcttg acctgaatac ttcattccaa
    39061 ttacaccata aattactaaa caaattaata atgatggtat agtcgaatat agcatatatt
    39121 ttatatgtgt aaacaaatct gttcctgcca ttgctggagc taagttagtc gtttcagata
    39181 aaggtgatag tttatctcca aagtaagctc cagatattat agaacctacg attaatccaa
    39241 ttggcatacc taaactttga ccaattccta aaagagctac tcctactgta cccattgtgc
    39301 tccaagatga acctgtcgca agagatacta ttgcacagac taatgttgat gctactaaaa
    39361 aaatatttgg tgataaaatt tttagtcccc agtaaatcat acatggaact actccactta
    39421 ctatccaagt tcctataagt acgcctatta taattatgat taagattgct tgcattgcca
    39481 tctttatact attaaacata cttaattcta actctttcca tttaaatcca agtctatata
    39541 cagccacaag tcctgctact gctgctgctc ctattagtgg tacatgaggt gatgaaccat
    39601 acactaatac tcctagactt aaaaataata tcaaacaagt aattggtatc attgcatcaa
    39661 acagacttgc ttttctacat tctcgctctt tcataaaata gtttcctcct aattatttaa
    39721 acactacact ataatttttt ttgaaacata tttgctcgta ctagatgaca tgataccccc
    39781 ataagttaat aaaaattcta aattgtcacc taatttataa tcatattttg tgttacttac
    39841 atctagtatc atgtggtctg aacttgcccc taatattttt atatcagtgt ctattggaat
    39901 taaagagtca atatttatat cttgtttacc tatagctatg attgcccttt ttaagattcc
    39961 cttatcttca taaacaggtt tatttctaaa ggcatcaact cctatttctc caataggaac
    40021 tgatggtttt tctttacatt caactacttg gcatattaat ttaaatgcat cttgatatgt
    40081 tccatttata ttttcaccgt atgccgtttc tctaccaagt agaatggact cgcctattct
    40141 caaattagta ataccttctg gcatattatt atttatcaac agatgaatag aacttgaatt
    40201 acctccagat actatttcta gatttattcc aaatttttct tccattcttt ttgctatact
    40261 taccagtctg cctatatttt ccctagatgg tataatagct ccataacagg ttaagtttgt
    40321 agctatacca attatttcaa tattttttaa tcttactatc tctcttacat tattaaaaaa
    40381 gtcttcttca taaaagtatc cttctcgtaa atcgcctaag tcaaacataa gaactatctt
    40441 atgaataata tttttacttt cacataattc atttaatttc tttattgttc ctaattctga
    40501 gttaaaacta atatcgcaat aattgataac ttcttctaat tcacttaaca ttgggattct
    40561 aatcaatatc ttaggtagat ttatatcttg taaatttttt aagttttgta ttcttgaatc
    40621 tgctatatgg tctattcctt cactacatat ttcttctact atttcttttt gtgcacaaaa
    40681 tgatttagta acaaaagata tcttaatatt tctttcatga cacatctgcg aaattaattt
    40741 tgcattatgc tttaactttt ctatatctat ttctaaccta ggatacataa tctttcctcc
    40801 tcaaaattca aactttttaa caggagctta agagatgctt cttcatattt tttagataaa
    40861 acacccatag atgccataat atagtcatta tctattaata tctttgtttt ttcctttgga
    40921 aatagtaatt cattattttt attgtatttg agcatattat ctaatatttc aattctacta
    40981 gggagcctag taagtgctcc ccctgtacca actatatatc ttacttctgt taaatctttc
    41041 ccttctgcaa ttttgacttt tccagttgtt ccatagatat ttcttatttt tcctgaatgt
    41101 cttagcatag ctttaacaac agcttctttt gtcaatcttt caacaaataa tatttcttct
    41161 tttgattttg gtattggagg ataattctct attactgcat caatatctat agatagttca
    41221 ctttgtaagt tttctttacc tataacttct actatattct tcatatttac atatacacct
    41281 aaatccccct caacacttct ctttgcagta ggttcaggat taaccaaaat tttattaata
    41341 tagtcacttc catctgtaac agaatgtaca tcagtagttg ctccacctac atccagagtt
    41401 aataaatctc caatattttt atataaaagt ttagaagctt ccattacagc tcctggggtt
    41461 ggagtaatat tttcattaac catttcttta atgtatttca ttcctggtgc tgtagttatg
    41521 tgttcttcaa atacactttg aatgattctt cttgtaggtt ctatattcaa taaatctatt
    41581 ttagggtata cattttcagt tatataaagc ttataattag tatcttcaaa tattaacctt
    41641 atttcctctt gattttcaac attaccagca tatataactg gtatgcctag attcatagaa
    41701 gctatcattt ctgcattata aatagctgta tctctttctc cataatcgac tccaccagct
    41761 attaaaatta tgtttgggtt tatttctttt attttattta aatcagttct tttaattcta
    41821 cctgatgtta tttgacgaat aacagctcca gcccctaaag ctgcttcttt agctgctttt
    41881 actgtcatat catagactaa tccatgaaca gtcattttta atccaccagc tgcactagaa
    41941 gttgcaaaca tatcattata ctctatatca tctacagata atttattagc taaatcttta
    42001 atagcacctg ataagccatt tcttacatcg ccaccttcaa acactgttgt tggagcttga
    42061 ccttgaccca aaaatatagg attgttagta ttaatatcgt ggaaagcgtt taccactgta
    42121 gtagtacttc caatttcagc taccaacaca tctattttta aagaaacctt gctattttcc
    42181 attatttatt actcatttct cttctttttt caactaagaa agtggctaca tgtatgcctt
    42241 tactgtttct tccaaatcca gcatctatgc cttgttcaac agctatttca ggtgttactt
    42301 gtgttcctcc acacgcaatc attactttat ctcttatacc tttttctaca caataatcgt
    42361 gtaatttttt catgttttta tagtgaatat catcatgact tattattgta gatgcaagta
    42421 ttgcatctgc attcagttca atcgctgcat ctaccaattt ttctacaggt acagaagtcc
    42481 ctagatagtg gcattctatg ccatattttt caatacctcc atgttttata tctattattt
    42541 ctcttaaacc aactgaatgt tcatcttctc caaccgtagc tgaaactatt ttcataggtt
    42601 tttcttctat atctgctctt atttcttcat cagttagtac cttaggttct tctggtatta
    42661 ctaaattgtt taaatctata tcaaacggaa ctcttccttt cacttctatt ctagtccctt
    42721 cagattcatg cataacttct ctacttatga cctcaatttc ttgtaagttc atttttttga
    42781 caaattccaa agctgcaaac tctgctgttc ttttatcagt aggaagcatc attgttatca
    42841 ttataattcc atctgcacac cattccattt caggcttaat tttagttcca tttctgtact
    42901 catatgattc ttttagtcta ttttctacat tatcaaaatc gtcaagctca tcaatatata
    42961 ttattttgtc tggattttca aatgtacatc catctattaa cattgatgga ttatctatag
    43021 cattttcatc atattgagct acattattgt atccaaagtg agcagttaca ggtgctaaat
    43081 aatcttcttc tctttcataa acagtacctt caccaactcc accttttatt tttctagata
    43141 ttccatcacc atttctttct ggataatttc cagaatcaac aaaaaatcct tgttccacag
    43201 attcaaaata tccaccaact tcagatattt cttccatata taatacagct ctttctttaa
    43261 gttctcttgc tttgtctctt aattcaccga catctttaag ttctatcata tccataagcc
    43321 catccatacc aactaaagct tgctttgctg tatcacatgc ttctatattg tatatatgcc
    43381 aaggtacatt tctaccttca tcaggtgtta tagttgattg tatgtcagct ctagttaata
    43441 ctgatgttaa taagtttaat acatgagtaa ctgttgcttc tctagttgat gattccatat
    43501 atttagtatt catttgagct ctcatcttat aatcactaaa taactctcta agtgcaacag
    43561 cgtaaggtaa gtctattttt aaacatggtg ctggaggtgc tgttggtgga actgttgata
    43621 agcatatatt acttttatct atacctattt tttctgagaa tatagagttt aaagcgtgtt
    43681 gaaccataag ttcaggcatt accttccatg cttctctagc agtagcatta gcattatgtg
    43741 ctccatcaat ttgagctata ttggcaaaag ccattatttt ctttgcctca caagcatcta
    43801 caaaagacct taccatgttt atattcctat acagaacatt atattgtggg tcttgatgtg
    43861 ctccattaac tccttcctca gcaaacatta ctgctacttc aggaccagca acaccactta
    43921 catatgaatg ataattaata ggtctaccaa cttcttcttc tataaaatca agagccttcc
    43981 tttgagctct tacttgtttt cttgttattg gaactccacc tataccttgt ggggttcctt
    44041 ctataagccc atcaaaatgt gattgtccag cagttctgat aaccattaga tggtcagctc
    44101 catgatgagc tgccattctc attcttctta tatcatcctc aaatctacca gaagctattt
    44161 cagtagtaat aactggactt ggttgagggt ctatattatt gaaatactta gatgatggta
    44221 gccctacact cttctttaaa ggttttgtac agtctttata tttaaaaggt cccatttcaa
    44281 gattttccaa gtgttctctc caaacccaac ctcttcgttt tggtttatat ttatccaaat
    44341 cttttaatat atagtctata tccaattttt tattttcctt taacatgatt ctaccccttc
    44401 tctaaataaa catgtaactt catcccaata ttcaccctta actaataaca aacctgcttc
    44461 tctaaccgtt atatttttag atttagccaa tttataaact acatgccctg ctcctttacc
    44521 tataagacct ctttccataa ccccttctaa aataggtttt acttcaagag aagaaaatcc
    44581 cattctcaac aagacagacc tttctattga tggtgttgta ttctttttac caagttctat
    44641 cattggttct actatctggg tagctaaact ccaaaacctt tcttttaact catcatcact
    44701 cagttcttga agatgttttc ttctaacttc aaaatcatcc tctctttttt tcatgaacat
    44761 tcctccattc gtattgattt aatcgaattt tctgaatttt tattttatat taaattaaaa
    44821 aacaataatt actataaatt atttaataca tcaacaacaa aatctcttga actttttact
    44881 tcttccatta aaaattctat atctcgatta cttagcttat caatattatc aatgtgatta
    44941 ttagatatac aattttttat aaatgacttt cttattttat tcatatcaat atcaacacat
    45001 ttcaataatt ctggtctttc tggtaatatt aagtttactc ctgcaatttc ttctcttgga
    45061 tttccaaatt ttatatctat tccattttct cttgcaaagg taagttgtgg acatatatgt
    45121 ttacctgcac ccgtatattc tgtttcttgt accactatag ctttatcctc atctagctct
    45181 tgagctaaag agaatgctgc tgctaaagat gtatttcctg ctggacctct ttctatacct
    45241 tctagctgtg ccaataattc agttgtataa aaaacttctc cttgttttac taatacatat
    45301 ctatccatat atctaagtgg tctagctgca gacctaggta catctgacct atcaggccat
    45361 gtacagtatg gtatcccaaa acctgtatgt cctgttgtaa atgatttttt attaaattga
    45421 ttatcactag ccatgtgtaa tccttttaaa tctacacttg ctcctactac tttagtatta
    45481 atagaaccag cttttatcaa tcctcttgca gttccagtaa gattaccacc acctgcattt
    45541 gtacaaacaa ctatatcagg gtctttttta tacttttctc taaattggat agctaactca
    45601 taacctaaag tttctacacc tgcaattcca aatggtgaat ataatgatgc gttaaaatag
    45661 cctgtttctt ctaataaaac taagaacgta taaaataact ctggtccaac agttaactga
    45721 acaacttctg ctccatatgc ctcacatttt cgcgcttttt ctatgatttc aggttgacct
    45781 acaccattag aatcataaca ttcttgaact attatacatt ttaatccctg cattgccgct
    45841 tgtgatgcta cagctgcacc atagttacca ctggtagctg ctattacacc tttatatccc
    45901 atttgtttgg catgatatac tgcagtagct gctcttcttg ctttaaaact accagatgca
    45961 ttcattgctt catcttttac aaatattcta gctcctttac cttctttagc acattttcta
    46021 gctaatttag tcaaattttt aagctctatt aaaggagtat ttccaacagc atactgtgat
    46081 tgaattgctt caatatcttg taatgtatat cctgtttcac tcatcatttt ctcataatca
    46141 aaccctattc cctcttgttc aaaagaagaa taatccaatc cgattgcatt tttcattatt
    46201 tcgttatttc ttcccataac agcttgataa ctcatatctt tcattgatgt tgaattattc
    46261 attatataca cctccaaata atatttcttt agcttggatt cctatttgga gtaactctgg
    46321 tatacattta ccataatcat gagtataata tggatttatt ttaagtaaat taccttttac
    46381 aagtctgcct gtaattgttt taacttctac taagtcgcct atagaagcat cactttgtat
    46441 aaaaccttta acccacattt ctaaggatac tttcttagta tcctctggaa cttgtggagc
    46501 tctttcttct ggagtcaata ctatattgtg tataataacc caatcattaa ttttagcatc
    46561 cattttttca tccccttatt aattgactat tgccttacta attaatttac tgttatttta
    46621 aattaaacat tattttacca attagctaac tgttatctta ctaattagct agttgctatc
    46681 ttactaatta gcctttgatt cttcttccaa ttcaacttct atctgatttc ttatatctcc
    46741 cattattgct ctaggtactg gtatatccaa catagtttta agacctggtc tagcatttat
    46801 tattaatgga atagagttta ctatcattgc ataagtacct actcctccag gtatttcagg
    46861 atttatttgt aaatcaatgt ttggtatacc ttttattgat acatagtctc ctgttttaat
    46921 accttctgct tctggtatta tctgttgtgg atgctccatt tcaattaaaa cttctccatt
    46981 tacatatcca tatccacatt gtctacaacc agcaacattt ccagccaatg cctctccgta
    47041 cttagatttt ctatctactg tagtcattat tggctctttt gtttgttcta tcttatctaa
    47101 tttccaacct aatccatcag ctatcatatt tattgattct acaaaaccta catgacctgc
    47161 tatagtctta tcttctacac cttttataaa ttcttccctt gttactccaa caccttgttc
    47221 caccataaca gactttccaa atggagataa atcatttact ctcttagctt ttatactagt
    47281 aacttcttca caagtacctg aaagtgccaa tactagtaaa tctaatacaa atcctggatt
    47341 tattcctgtt ccaagtatac ttactccatt ttcctttgca acttcatcca tttttttagc
    47401 taaatctgca tcatcagctt gtggatatgc catttgttct gctgttgata taacatttat
    47461 ttttctattt aataagaaat ttattttatc aaaagccttt ttagtaaatg aatcagttgc
    47521 taacaaaaca acatctacac ttttttctct aaaaacttct tcataatttt tattgataat
    47581 aacttctggt ctttctcctc tttcaattcc taaaacatca tacatacttt tccctgaagt
    47641 acttctacta caaacagata ctatttctat accttctttc tttagaatca tattagccat
    47701 acctattccc atagctccaa atccccatat tcctactctt acttttctca tatatacacc
    47761 ctcctaaaaa tttatatatt ctcaataatt agttaaagca taaatcgtgc caactttaaa
    47821 attaaagtaa atatttattg gaacgttgaa actataatta ttttattatg tattttcata
    47881 aataaaaaat aatatttctt atgagaaata aaaaaaagag caaaatattt tgctctttta
    47941 taataatttt gctcctctat tttgcttttt tattttactt tgattttgtt tttttatctt
    48001 gcttatttat aatattttga tattatattt ttttatttta tactgtaaat tttgcctatt
    48061 catacctaaa attgatgcag ctttagttat attgtaagat gccttttcta atgattcata
    48121 tatatacttt tcttctatct cactgataat cttactaagt gttttgtcct ccaaatcgtt
    48181 taaagaataa ttgttactct ctttattctc tttaagagtg taatagtcgt ttagattaat
    48241 catacttgtt tctatttgta cctcatcttc catcatactc atagtacaat aaattatgtt
    48301 ttctagctct cttatgttac cttgccaatc gtaatttttt aaaattttaa tagcttcatc
    48361 tgaaattcct accacttttt tatttaactt tttattatac tttgaaataa attttttgca
    48421 caatagaggt atgtcctctt ttctatctct aagaggagga atttttatat gaattatgtt
    48481 aagcctataa tataaatctt ttcgcatttt atgctcgttt aaaattacct ttggactttc
    48541 atttgtcgtt gcaataattc ttacatctat aggtatatcc ttagttcctc caacccttct
    48601 tacataacct tcttgtaaaa cccttaatag tttagcctgt aacataatag gcatggaatt
    48661 tatctcatcc aataacaatg tccctttgtt agcttgttca aataagcctg gcctatctat
    48721 tgctcctgta aatcctccct tgctagttcc aaaaagaata ccttcgaata gtgattccgg
    48781 caaagctgca caattttgtg ctataaatgg atagtttctc ctattacttc catagtgaat
    48841 ggactggcta aataactctt tacccgttcc agtttcaccg tatataaata ctgaagcatc
    48901 agacttgcaa gctttcttag atttagctat tgcttcttta atcaaattac tttcccctaa
    48961 aatatcatca aattcataac cctttttttg tctaatacta actttattga ctaaattttt
    49021 tttataattt ctatttagta aattttctat cgttgtcata ttctttgata tttcaagtgc
    49081 ccctacaaac tcgcctttaa tatcaattgg tattgtagta ttaattgctg ttatctcttt
    49141 tccattttta tccagatatt tttgaatatt gtctttgata acttcccttt tttccaaaac
    49201 ttctaatagt gtactcctgt ttattttcat tttgttgact atatcaaaaa atgaattggt
    49261 taaaatatcg tgtctgttca ttttttccat tttttgtaca gaattattgt agattatagt
    49321 tttcccgtct ttattaacaa aatgtacccc ttcatcaata ttttccaata tttcattcat
    49381 tattttaaaa aatatctctt ggttcaacta tatcacccca gtacctaaat atataaaaac
    49441 ccaaaaaatt aataaaatgt tgctattgta tttatcttat catatattta tgatattttt
    49501 tatgtacatt atattaatat atttttaagt atatttaaaa ttttatttat atatctttca
    49561 ttatggttat atacaatatt tgaattgtca ttaaatatgc caatagtttt atatattatt
    49621 gattttat
//
LOCUS       DBGJFU010000014        45730 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.014, whole genome shotgun sequence.
ACCESSION   DBGJFU010000014 DBGJFU010000000
VERSION     DBGJFU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.014"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            23..1090
                     /locus_tag="ACOIAS_000802"
     CDS             23..1090
                     /locus_tag="ACOIAS_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HGZ2700522.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1260..3161
                     /gene="cooS"
                     /locus_tag="ACOIAS_000803"
     CDS             1260..3161
                     /gene="cooS"
                     /locus_tag="ACOIAS_000803"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HGZ2700523.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3154..3627
                     /locus_tag="ACOIAS_000804"
     CDS             3154..3627
                     /locus_tag="ACOIAS_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2700524.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3602..4828
                     /locus_tag="ACOIAS_000805"
     CDS             3602..4828
                     /locus_tag="ACOIAS_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2700525.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(4967..6154)
                     /locus_tag="ACOIAS_000806"
     CDS             complement(4967..6154)
                     /locus_tag="ACOIAS_000806"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2700526.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTRENMYN
                     CSK"
     gene            6427..7251
                     /locus_tag="ACOIAS_000807"
     CDS             6427..7251
                     /locus_tag="ACOIAS_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGZ2700527.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7380..8645
                     /gene="gluD"
                     /locus_tag="ACOIAS_000808"
     CDS             7380..8645
                     /gene="gluD"
                     /locus_tag="ACOIAS_000808"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HGZ2700528.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8858..9448
                     /locus_tag="ACOIAS_000809"
     CDS             8858..9448
                     /locus_tag="ACOIAS_000809"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2700529.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            9778..10911
                     /locus_tag="ACOIAS_000810"
     CDS             9778..10911
                     /locus_tag="ACOIAS_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2700530.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
     gene            11827..12849
                     /locus_tag="ACOIAS_000811"
     CDS             11827..12849
                     /locus_tag="ACOIAS_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGZ2700531.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            13146..14066
                     /gene="pyrB"
                     /locus_tag="ACOIAS_000812"
     CDS             13146..14066
                     /gene="pyrB"
                     /locus_tag="ACOIAS_000812"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGZ2700532.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            14105..14797
                     /locus_tag="ACOIAS_000813"
     CDS             14105..14797
                     /locus_tag="ACOIAS_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HGZ2700533.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            14790..15692
                     /locus_tag="ACOIAS_000814"
     CDS             14790..15692
                     /locus_tag="ACOIAS_000814"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGZ2700534.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            15777..16361
                     /gene="pyrE"
                     /locus_tag="ACOIAS_000815"
     CDS             15777..16361
                     /gene="pyrE"
                     /locus_tag="ACOIAS_000815"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGZ2700535.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            16576..17502
                     /locus_tag="ACOIAS_000816"
     CDS             16576..17502
                     /locus_tag="ACOIAS_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HGZ2700536.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(17554..18444)
                     /locus_tag="ACOIAS_000817"
     CDS             complement(17554..18444)
                     /locus_tag="ACOIAS_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2700537.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            18632..19663
                     /locus_tag="ACOIAS_000818"
     CDS             18632..19663
                     /locus_tag="ACOIAS_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HGZ2700538.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            19957..20832
                     /locus_tag="ACOIAS_000819"
     CDS             19957..20832
                     /locus_tag="ACOIAS_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HGZ2700539.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            20850..22340
                     /gene="cls"
                     /locus_tag="ACOIAS_000820"
     CDS             20850..22340
                     /gene="cls"
                     /locus_tag="ACOIAS_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGZ2700540.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            22533..22817
                     /gene="groES"
                     /locus_tag="ACOIAS_000821"
     CDS             22533..22817
                     /gene="groES"
                     /locus_tag="ACOIAS_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGZ2700541.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            22850..24478
                     /gene="groL"
                     /locus_tag="ACOIAS_000822"
     CDS             22850..24478
                     /gene="groL"
                     /locus_tag="ACOIAS_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGZ2700542.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            24740..25480
                     /gene="larB"
                     /locus_tag="ACOIAS_000823"
     CDS             24740..25480
                     /gene="larB"
                     /locus_tag="ACOIAS_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HGZ2700543.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            25615..26747
                     /gene="larC"
                     /locus_tag="ACOIAS_000824"
                     /pseudo
     CDS             25615..26747
                     /gene="larC"
                     /locus_tag="ACOIAS_000824"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
     regulatory      26807..26908
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            27129..28664
                     /gene="guaA"
                     /locus_tag="ACOIAS_000825"
     CDS             27129..28664
                     /gene="guaA"
                     /locus_tag="ACOIAS_000825"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGZ2700544.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            29242..30633
                     /locus_tag="ACOIAS_000826"
     CDS             29242..30633
                     /locus_tag="ACOIAS_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2700545.1"
                     /translation="MRNKIIIGITFSLVLLMSGCASKQTAEINVLATTDLHGVIPYEL
                     TSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISK
                     DMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYII
                     KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVE
                     DAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQ
                     IEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDKSSKLIKFEKNQES
                     DKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISS
                     PKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEK
                     GKQEYSINLKHIQ"
     gene            complement(30781..31089)
                     /locus_tag="ACOIAS_000827"
     CDS             complement(30781..31089)
                     /locus_tag="ACOIAS_000827"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2700546.1"
                     /translation="MLREQGSAIRNTLDSVLSLSNQKAYPVWESVNSFALIKAAQAGL
                     GITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNITQPLKIILDNISNVL
                     "
     gene            31126..31647
                     /locus_tag="ACOIAS_000828"
     CDS             31126..31647
                     /locus_tag="ACOIAS_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HGZ2700547.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(31776..32609)
                     /locus_tag="ACOIAS_000829"
     CDS             complement(31776..32609)
                     /locus_tag="ACOIAS_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2700548.1"
                     /translation="MSWKHIKIPNGDTMIFCNSFITRKSSMNIVVTHTPIVTTLEMQK
                     AYEPLAKHNVNIFAFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYG
                     NTGIGGMFAQYYACSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHI
                     TVKPPKYKGYNEVYDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIP
                     TLVFKTLHDRYFKTQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVEN
                     Q"
     gene            complement(32794..36222)
                     /gene="uvrA"
                     /locus_tag="ACOIAS_000830"
     CDS             complement(32794..36222)
                     /gene="uvrA"
                     /locus_tag="ACOIAS_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGZ2700549.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHQDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLKGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            36541..38775
                     /locus_tag="ACOIAS_000831"
     CDS             36541..38775
                     /locus_tag="ACOIAS_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2700550.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            39031..41061
                     /locus_tag="ACOIAS_000832"
     CDS             39031..41061
                     /locus_tag="ACOIAS_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2700551.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            41058..41510
                     /locus_tag="ACOIAS_000833"
     CDS             41058..41510
                     /locus_tag="ACOIAS_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2700552.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            41512..42567
                     /locus_tag="ACOIAS_000834"
     CDS             41512..42567
                     /locus_tag="ACOIAS_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGZ2700553.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            42634..42942
                     /locus_tag="ACOIAS_000835"
     CDS             42634..42942
                     /locus_tag="ACOIAS_000835"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGZ2700554.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            43046..44311
                     /locus_tag="ACOIAS_000836"
     CDS             43046..44311
                     /locus_tag="ACOIAS_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HGZ2700555.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            44399..45115
                     /locus_tag="ACOIAS_000837"
     CDS             44399..45115
                     /locus_tag="ACOIAS_000837"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2700556.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(45387..>45730)
                     /locus_tag="ACOIAS_000838"
     CDS             complement(45387..>45730)
                     /locus_tag="ACOIAS_000838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700557.1"
                     /translation="KENTSIIGVIFPSKFTYALGPVATALSMQYYAINFGDSGIAVIG
                     LNNVTGKLENEAFLFVPKEEVTSVKFNKKLMSYELEISTSQGTLAFKVNKTMVGASWH
                     KENLATILKSL"
BASE COUNT        17614 a         4996 c         8033 g        15087 t
ORIGIN      
        1 attaatacaa aggagattta ctatgaaaaa taaattagtt tacatatgta ttatgagtct
       61 attgctattt gtagtaggat gttctaataa tccaaataag gaaagtacca ttaaaaaaga
      121 ctctaagaat aaccaaagcc aagttaaaga agaaaaaaga gaacctgtga aaaaagatat
      181 aactataagt ttcgctggtg atgtaactat ggggaactat aaaggttcat catattatgg
      241 cagttttgac catgaattta aaagacaagg agaagattac aattattttc ttaaaaatgt
      301 aaaacctgta tttgaaaaag atgacttaac agtggttaat ttagaaggtc cacttactac
      361 agcgtcaaat gcaaaagaaa aaaaatttgc atttaaagga aagcctggat atgtaaatat
      421 tttaaaatct ggaagtgtag aggcagtaag tattgcaaat aatcattcag aagattattt
      481 tgaagaagga atgaaagata cacagtttat attggacgag aataaaataa attattttgg
      541 attagggaaa gatgctgttg tagatgtcaa aggaattaaa gtgggtctat tagggtataa
      601 tggattatca actgaatata atgaaaaaaa tttaaaacaa atggaagatg atattaagtc
      661 cttaaaaaag aaatcggatg ttgttgtagt gtactttcat tgggggatag aattagatta
      721 ttatccagat aaaaaacaaa aagattttgc tcattatgct atagacaagg gagcagattt
      781 agttatgggg tctcacccac atgtgattca aggaatagaa aagtataaaa ataagtatat
      841 agcttatagt ctagggaatt tttgctttgg aggaaataag aatccttctg atacagatag
      901 ttatatatat caacaaacct ttactttttc agataataaa ttaacttcaa ttaaagagcc
      961 aaatataata ccaacttcaa taacatctag taactccaga aataattatc aaccaaaaat
     1021 attggatgga agtgagaagg atagagtttt aaataaactt gaaaaaataa gcaaagattt
     1081 aaataaataa aatgtaaaaa aaaataaata tatatattta ttttaataat ttatattttg
     1141 tttagggtat ggtaaatcgt ggtattaaat aagtagattt taatagttaa attatagtca
     1201 attataataa agctatttaa tctacttttt taatataaaa atataagggg gaatttgtaa
     1261 tggcttgtaa cacttgtaaa atgtgcgaaa gtgcagacaa gaaattagaa agttttgtag
     1321 cttcaaagga tgttgaaaca gcatttcata gaacagaaga tcagaaagtt aagtgtggtt
     1381 ttggacttca gggggtttgt tgtagactct gttcaaatgg accatgtaga gtaactccta
     1441 aatcaccaag aggtatatgt ggagcagatg cagacactat agtagcaaga aactttttaa
     1501 gagcagtagc atcaggagca gcttgttatt tacatgtggt agaaaataca gctaagaatt
     1561 taaaaaatgt tggaataact aaaggtgttg ttaaaggaga aaaaacatta aatcaattag
     1621 cagagatgtt tggaatagaa agtgatgaaa agtatgataa atgtataaag gttgcagaca
     1681 aagttatcaa cgatttgtat aaatcaagag atgataaaat ggaattagta gaaaaaatag
     1741 cttatgcacc tagagtgaaa aagtggaaag agttaggtat aatgcctggt ggtgcaaagt
     1801 cagaagtttt tgatgcaata gtaaaatcat caacaaattt aaacagtgac ccagtagaca
     1861 tgctagttaa ttgtttgaac ttaggtatat caacaggatt atatggtctt acactaacta
     1921 atcttttaaa tgatgttatg ttgggagaac cagttataag aatggctcct gtaggattta
     1981 atgtaataga cccagactac ataaatataa tgataacagg tcatcaacat tcaacatttg
     2041 caaatttcca agatagatta aaagatgaag atgttataaa attagctcaa tctgttggcg
     2101 caaaaggatt taagttagta ggatgtacat gtgttggtca agatttacaa ttaagaggag
     2161 aacattatca agaagtattt gctggacatg caggtaacaa ttttacaagt gaggctgttt
     2221 tatcaactgg agcaatagat atagttcttt cagaatttaa ctgtactata ccagggcttg
     2281 aaccaatagc agataaatat aaagtaaaaa tggtttgttt agatgatgtt gctaaaaaag
     2341 cgaatgcaga atgcataggt ttagacagaa gtaaattaga tgaacttagt aacactttaa
     2401 tagaaaaagc attggagtct tataaggaga gaagaggaag tattgaaata gatataccaa
     2461 aagaccatgg atttgaacaa tctttgactg gtgtaagtga aaagaacttg aaagcgtttt
     2521 taggagattc atggaaacct cttattaatt taattgcaga aggaaaaatt aaaggtgttg
     2581 cagctgttgt aggatgttca aatatgacag caggaggaca tgatgtaaat actgttgaac
     2641 ttactaaaga attgattaaa aaagatataa tagtactatc tgcaggatgt tctactggag
     2701 gattagaaaa tgttggtctt atgtcacctg gagcagaaga attggcagga gaaaacttaa
     2761 aagaagtatg taagacttta ggaataccac cagtattaaa ctttggacca tgtctagcta
     2821 taggaagact agaaatagta gcaacagaat tagcagctga acttggaatt gacctaccac
     2881 aattaccatt agtgctttca gctccacaat ggttggaaga acaagcatta gctgatggag
     2941 cttttggtct tgcccttggc ttacctcttc atttagcttt acctccattt ataacaggag
     3001 gaaaattggt aacagaagta ttaacagaaa aattaaaaga cttaacagga ggacatgtta
     3061 tagtgaatcc agaccctaaa tcttcagcta atcaattaga agaaataata atagatagaa
     3121 gaaaaaattt agggttaaag gatgtggaat gcaatgcata gaatttttat aaataaagat
     3181 ttatgtacag gatgtaagag ctgtgtacta gcatgtatgc taaaacacaa taaagattat
     3241 gacatgtaca ctcttgattt agaaaatata gataatgata gtagagggca tatagaatta
     3301 gatagtagac ataataatcc agttccaatt ctttgtagac actgtgatga acctgaatgt
     3361 gtattagcat gtatgagtgg tgcaatgcat aaagatagtg aaagtggaat cgtttcatat
     3421 gatgaagaga aatgtggttc atgttatatg tgtgtaatgt cttgtccata tggattatta
     3481 aagccagatg atagaagcaa gcagaacata cttaaatgtg atttatgtaa agatgaggag
     3541 tatcctagat gtgtagctaa ttgcccaagt ggagcaattg aattacagaa ggaggaaaat
     3601 gatgaattat gtagtgttag gggctagtgc agctggtata aatgcagtaa aaacattaag
     3661 agaattagat aaagattcaa atatagtagt aatttctaaa gatgaaaacg tatattcaag
     3721 atgtatgttg catcacgtta tatctgaaca cagaacttta aaacaaataa attttgttga
     3781 tgaagatttt atggagcaaa ataatgtaaa atggattgca ggtaagactg ttaaaggtat
     3841 agacataaat aaaaaggtag ttcagacaga agacattact gtaaattatg ataaactttt
     3901 aatagctaca ggtgcatctt ctgctattcc tcctataaaa aatttaagag atggaaattt
     3961 tgtatattct gtgagaaaca tagatgatat ttataaaata aaagaaaaag cacaaaattc
     4021 taaaaatgta gttataatag gtgctggttt agttggtata gatgcattag tgggattatt
     4081 taaatatgag cagttaaata tttctgtagc ttttatggaa aagtacattt tagatagaca
     4141 attggatgaa tatacagcat cagtgtatga aaataagttc aaagaaaaag gtgtaaaatt
     4201 ttacccaagt gcatcaatac aagaaattgt attggataac agtaaaaatg ttacaggtgt
     4261 tgcattttca aatggtgaag ttttagatgc agacatggta attgtagcta caggggttaa
     4321 accaaatgct gactttttag atggaacagg aatagaatat gatagaggta taataataga
     4381 tgatatgtgc caaacaacac aaaaagacat atatgcagca ggagatgttg taggtaaaaa
     4441 tgctatttgg cctctggcag taaagcaagg aattgtagct gcctataata tggttggaaa
     4501 agataagaag atagaagatg aatttgcttt taaaaatagt atgaatttca tggatatacc
     4561 aactatttct ataggaatga atacacctgt agatgatagc tataaagtat taaccagaca
     4621 tggaattaat gattataaaa agtttgtttt taaagataat gtaatctatg gagcagttat
     4681 acaaggagat atttcatatg taggtgtatt aacttattta ataaaaaata aagttgaaat
     4741 atatgattta gaaaatagga tatttgatat atgttatgca gactttttta atataaaaga
     4801 aaatggagaa ttttgttata gtgtatagta tatacgtgga gtaaggttta taaatacagt
     4861 aaatcaataa atagagttat tctaaaataa gaaaaatgac aaatcttatt tagaaatagc
     4921 tctatttttt gtaattaaat ttaaattata ttcaatttgt aaaatattat tttgaacaat
     4981 tatacatatt ctctctagtt agaggtaaat cattttttat acctttagtg aaaagtattt
     5041 gatgtatatc tataatacca gtgttgaaag cagcagcaca agaacaaagg tataaatccc
     5101 acattcttat aaacttatca tcaaacattt ctttaatctt tggaagattc tcagagaaat
     5161 ttttttgcca gtatagtaag gtttttacat aatgtaatct taaactttct acgtctatag
     5221 tatgatattt gtaatcacta caaatatgta tcatttctct taggctagga ataactcctc
     5281 ctgggaaaat atattttttt atccaagggt ctccataaga ttcctgcaat ccacttatat
     5341 agtgaagtaa aaacaatcca ccatctttta aaacagcatt tacgttttta atgaaaagtt
     5401 catagttttc tctaccaaca tgttcaagca tcccaacgct tacaactcta tcaaacataa
     5461 ggtctgattt ttttaattct ctatagtcca taagtcttac ctcaagatag tcttgaaggt
     5521 tctcttcttt aattcttgcg ttaaattcct taaattgctc ttcgctaagt gtaattccaa
     5581 cgccgtgaat tttatatttt tttgcagctt ctattagtaa aaatccccaa ccacaaccaa
     5641 tatcgagaag gctcatacct tcctctaaat ttagtttagt taatatgtgg tgaactttat
     5701 tgacttgagc atcataaaga gtatcattat catttctaaa gtatgcacaa gaataactaa
     5761 gtgtcttatc taaccataag ctataaaaat cgttgccaat gtcatagtgg gcatgtattt
     5821 ctttcttttg atgtctcatt gaatttgatg agtgcaatag atgttttaca gctgaattat
     5881 caatattgaa tttgtctatt tgttttaaaa gtagatttaa agttaagaat aaatctccct
     5941 caacttcaat gtctctattt atgtaggctt ctccaagtgc taaagatgta ctttttaata
     6001 gttcagactt gctaatatca ttatttacta taactttgaa ctgaggggag ccctctccaa
     6061 ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg tgtttcacca aatttctcta
     6121 aatagtcttt taaaaaattt tttgtaatat tcatattacc agctccttct aatgtaaatc
     6181 cagtaagttt ccctgcttaa aatatattat agtccttttt ttaaaaaagt aaaatttttt
     6241 caataaattt tttatttaaa ggaaatttat tttttgtaaa gaaatttcat gttgtagtat
     6301 attttttgct tataaatttt ggtactggat ataatattaa actgttaatg atataataaa
     6361 tttaatctat aaagtaaata aaattttgta ataaatagtt aaagctattt tataaggaga
     6421 tatatgatgg aagtatatgc tttggttggg tcaagtggca cagggaagag ctttaaggct
     6481 ttagagttcg catatgaaaa tgatatagaa tatataattg atgatgggat tttaatatac
     6541 aaaaacaagg ttttagctgg gatatcggca aaacaagcaa atactactat agaagcagtt
     6601 aaaagggcta ttttttataa tctggagcat agacaagaag ttagagaagc aattaaaaaa
     6661 gaaaatataa gaaaaattct tattattggg acatcaaaaa aaatggttaa tcaaattgta
     6721 gagagattgt caattggaag ggtttgcaag tttataaata tacaagatat aagtactaaa
     6781 tatgaaatag aaatagcaaa acaagctaga aaagaaggaa atcatgtaat tcctgttcca
     6841 gcagttgaga taaaaagtat ggcaagtggt cttagtataa attctttgaa gagacttttt
     6901 agaaaaggaa ataataggaa tatggtagtt ttagaaaaaa caattataag accaactttt
     6961 agttatatag gaaagtttta tattagtgct gatgtaatta aacagattat tgaatatgaa
     7021 gtatacaact ttggaagcat tgatagaata aataaaatca atatagagaa ttgcaataat
     7081 tttatgaata tttttgtaag tataaatatc aatgatttag aaactataaa aaaaattgaa
     7141 gacatacaac agacaataaa aaagtctatt gagaaaatga ctttagttaa tgtacaaaaa
     7201 atagatatta acattcataa aattaaaaaa ataaacttcc tccaatgtta aaaaataaac
     7261 tgagaaaatg atatactaat ttgcaataaa gtgtgctata attaaactgt aaatttagga
     7321 aatggttttt tctaaatgga gtatacataa ttataacgta tttataaggg ggctttcaaa
     7381 tgtcaggaaa agatgtaaat gtcttcgaga tggcgcaatc tcaagtaaaa aatgcatgtg
     7441 ataaattagg tatggaacca gcagtttatg aattattaaa agaacctatg agagttatag
     7501 aggtttcaat tccagtaaaa atggatgatg gttctataaa aacttttaaa ggatttagat
     7561 cacaacataa tgatgcagta gggccaacaa aaggtggaat aagattccat caaaatgttt
     7621 caagagacga agtaaaagct ttatctatat ggatgacttt caagtgttct gtaacaggta
     7681 taccatatgg tggaggtaaa ggtggaataa tagtagatcc atcaacttta tctcaaggtg
     7741 aattagaaag attaagtaga ggatacatag atggaatata taaattaata ggtgagaaag
     7801 ttgacgttcc tgcaccagac gtaaatacta atggtcaaat aatgtcttgg atggttgatg
     7861 agtacaataa attaactgga caaagttcta taggtgttat aactggtaag ccagttgaat
     7921 ttggtggttc tttaggaaga acagctgcta ctggatttgg tgttgctgtt actgcaagag
     7981 aagctgctgc taaattagga atagatatga aaaaagctaa aatagctgtt caaggtatag
     8041 gaaacgttgg ttcttataca gttcttaact gtgaaaaact tggtggtact gttgtagcta
     8101 tggctgaatg gtgtaaatca gaaggttctt atgctatata caatgaaaat ggtttagatg
     8161 gtcaagctat gttagattat atgaaagaac atggtaactt attaaacttc ccaggagcta
     8221 agagaatatc tttagaagag ttctgggctt cagatgttga tatagtaata ccagctgcat
     8281 tagaaaactc tataactaaa gaagttgctg aatctataaa agctaaatta gtttgtgagg
     8341 ctgctaatgg accaactact ccagaggctg atgaagtatt tgctgaaaga ggaatagttc
     8401 ttactccaga tatattaact aatgctggtg gagttacagt ttcttacttc gagtgggtac
     8461 aaaacttata tggatactac tggtcagaag aagaagtaga gcaaaaagaa gaaatagcta
     8521 tggttaaagc atttgaatct atttggaaaa tcaaagaaga gtacaatgtt acaatgagag
     8581 aagctgctta catgcattca attaagaaag ttgctgaagc tatgaaatta agaggatggt
     8641 actaagattt actcttaata aataatagga tataattatt taagatataa aggggctgta
     8701 catagtacag cccttaaatt atgtggctta caatgaagta agctattttt gttttaactt
     8761 ataaataact tgataaatta tgtaatttag aatgtatttt ttaccaccac atgattacaa
     8821 attagaggag ttagattgaa tttttagaca tgaacttatg cattttaaaa gcaaagacct
     8881 taatttaaag tatctagtat tatttttaaa aatagtatat tggtttaatc catttatatg
     8941 tataatggaa aaatatatgg atttggattg tgaactttat tgtgatgaga gagttttaaa
     9001 aaattgtact gtacaacaaa agcaagaata tgctctaact attgtaaatt caatgaaaag
     9061 aagtacaaat ttttcaaata aatttgtagc tggattaaat aaacaatctg atattagaaa
     9121 aagagtaata aacatgttca gtgaaaaaca taaaaatggt atcttaaggg ctttgattat
     9181 aagcttgttt tcttctatta cattttttaa attcgattca actagtatta ttgaagagaa
     9241 tgatttctta gagaggtcaa gatatacgtt tgaaggaagg caagaagaca tgtttgtaac
     9301 aatagatttt atatatgcag atgtacctat aaaacttaga aaagagcatg aagaaaactg
     9361 tagagtcaat ggaatagtac caaaagattc agattcaata gaaattattc ctagatttta
     9421 taaagattgt atggatttaa aaaaataagt tagacttaat attgatgttg atattatgaa
     9481 ttttttatat ttaagcaatt gaaaatttta ttatagattc aattttatat gtgtttaagt
     9541 atgaaattta tagatagttg aaataattaa atatgattga tgcataggaa tcattactaa
     9601 gctataatta attatagggg agtttaaata aattatgagt tacacaatca taaaatgata
     9661 aaaatagtta tctgttacaa aaaatgtatc tttttaatta ataaagataa tatgaaaact
     9721 taaaaggcaa ataaacttaa atataaaata ctcttaaata agataggaga aaaatgtatg
     9781 tctaacttta ttctaaatgg gatttttaga acagttatag taagtagtct aagcattctt
     9841 ctgatattga ttttcaagaa aaatgtattt aaaagattta gcaaaaaatt taattattat
     9901 atatggatga tagttgttat taaattattt ttaccattta catattatac ttttacaata
     9961 aatatcttga gaagtaaaaa acacattaat attaataaca taaatttaga gggatttaat
    10021 aatgtttcaa ctataagtaa taatattata ttgtacactt ggattataac tgtagtagtg
    10081 tatttaattt acactatatt taaatatata aaacttaaaa atttaataaa tgacttatct
    10141 tatgatgttg atgatagaga gattgtaaat ctttataaaa atatattaga agaatttaat
    10201 ataacaaaag atataaaact caaatactct tatgaagttg aaacaccagc attttttaat
    10261 tcatgtgtac ttttgccacc acatgagtat aaattgaaag agttagactg gatttttaga
    10321 catgagctta tgcattttaa gagtagggac ctttatttaa aatatgtaat cctattttta
    10381 aaaacagtat attggtttaa tccatttata tatataatgg ataaacatat agatttagat
    10441 tgtgaacttt attgtgatga aagagtttta aaaaatcgaa attcagagga aaaaaaggat
    10501 tatgctttaa caattctaaa tgcaatgaga aaaggttcaa atacttcaaa taaatttata
    10561 gctggacttc ataagcaatc tgatattaaa aagagagtta tgaatatgtt taatgaaaag
    10621 tacaagaatg gtatattaat ggcattaact ttatgtttat tatcttctat tacttttttg
    10681 aaggttaatt caactagtat tgttaatctt aatgtattac ctaaaggatt tgaagactta
    10741 cgaactgtta gcagtatacg tttttcagtt ataggattta catatgcaga tgctcctaaa
    10801 aatatgaggg aagaatataa aataaattgt gaatcattag gtttgatacc taaagactct
    10861 gataaaatag aagttggtat agaatattat gacagtctat tgaatgatta aataaatatt
    10921 attttaacag atattaatta taaagttttg aatagtaatt tgttgagaaa taaatatatc
    10981 cttataaaaa tagatactta tataccaaga ataaaaaata gtcaagatag tacacaagga
    11041 tatacttata aagaacttaa ataagtgaaa taaattatta attataaggt ttataatttt
    11101 aaaaaattag ataaattcag gtgtaaaata ctataaaaca caattcggaa aagtgtatgt
    11161 tgaacttcat ttaaaatgga atttcaaagt tgctatagta agtagtttaa gcattcttct
    11221 tatattaatc tttgagaata atgtatttaa aaaagttagt aaaaaattta actattgtat
    11281 attaatgata gttgctattg atatattatc taaaaaaatt aagtatgaaa agattactgc
    11341 taggacaatc ttttcatact taatttttaa tatttatatt atccaaaatt ttcatctttt
    11401 taacttagaa tttgagaatc actattagga aataggcttt gatttatgta gggttaaaaa
    11461 tttcacagaa tatctaactt tatactaaat ccatcaaaaa atatacgcca tacaaaagcg
    11521 cataaaaaaa cacataaaac aatatttatg cgcactttaa cctcgaatgg tacaaaattc
    11581 tttactttta cagacccata ttatataatt aagtaaataa tgtatattta ggtgacatat
    11641 tggataagct aatttattta ttttattaat ataataatac tataaaagtt atataaagtg
    11701 gtttacaaaa aagttacaga tagagctttt attaatgtat tgtgtattag tattttatcc
    11761 ttaggtacac ttcacctaca aaatagaatc ataagataat tatgggaaaa ttttaggagg
    11821 tttatcgtga agaaaaagat attaatacca gtatttgcat cagttatggc attatcagta
    11881 agttcaattg taaatgcaga tgaagtaaat gattcatctc aaaataagga tgataaaaca
    11941 aatactgaat taaatttagg tgaatataaa gaagtaaaat ataaagttgc aaaaataaaa
    12001 gatggagtag ctataaagat aagagaagaa ggtcaagttc aaaacatagc ttattctgga
    12061 gatgaattta cggttttagg aactcaagga gaatgggtta aggttaaagt tgaagatggc
    12121 gaaggttggt tagctactag atatgtagat atatctgaag gtgtaggata tactaacgct
    12181 gataaagtaa acttaagaaa agataagagt gaaagttcag aagtaattga agagctggaa
    12241 aaaggaagtt ctttattggt tttggaagat aatggagatt ggttaaaagt taaagatggg
    12301 gaaacagaag gatatgtaaa atcatcttat atatctgata aagctcctgt aatagaagag
    12361 ccacaagtaa acccaagtat tgaccaaaat gtagattcta caaataataa tcaacaaaac
    12421 aatgcaaata ctaatcaaca aaataataat tcaaatgtgc caacagctaa ctctaatgca
    12481 gttcaagctg tacttaactt ggcatattct aagcaaggtt gtccatatgt ttggggagca
    12541 gaaggaccaa acacttttga ctgttctgga tttacacaat atgtgtatag aaatgcagta
    12601 ggaaagaata taccaagaac atctaaagca caatctaaat atggacaaac tgtaagtaaa
    12661 gcaaatttac aaccaggaga tttagtattc ttcacaacta atggttctgg ttctgttagt
    12721 catgttggaa tatatgtagg tgggggtaat atgatacact caccatcaac aggaaaaaca
    12781 gttagtgtaa ctagtataaa ttcatcatac tatacagcaa gatttgttac agcaaaaaga
    12841 atattataat cggtttgtaa tatgacaaat tgataaatac ataaatatat ataaacgctc
    12901 tatattatat ttaatttaat catggaaaca aaataatgat tatattttaa atgtactata
    12961 gagcgttttt gtatcacttt aaatctaatc aagattcaag ttaatatgac tgttaaatag
    13021 gagccttttc atatgatgat tatttgtgca atcccttgac aggatagggt aaaggtggta
    13081 agctactaac agtacaaaaa tacttttaat tttagttcag agaggctaaa aaaggaggac
    13141 gaaagatgtt aaaatcaaga aatttaatcc aaccagaaga cttttcaata gaagaaatag
    13201 atgaaatatt agaattagct caaaagataa ttgacaatcc gtcaaaatat tctcgtatat
    13261 gtgaaggaaa actattagct acattatttt atgaaccatc aacaagaaca agattaagct
    13321 ttgaatctgc tatgaacaga ttaggaggac gtgtagtagg cttttcagag ccaaattcat
    13381 cctctgcatc aaaaggtgaa actctaggag atacaatgag gatagtatct ggttatgttg
    13441 atataatagc tatgagacat cctcaatcag gtgcagcttc agaagcagct agatatacgg
    13501 aagtgccatt tataaatgca ggagatggca aaaaccagca tccaactcaa acacttacag
    13561 acttacttac tataaaatca ttaaaaggaa ctttagaaag ccatacaata ggattatgtg
    13621 gtgatttaaa atatggaaga actgtgcatt ctttggttaa ggctatggca agatataaaa
    13681 atacaaagtt tgtatttata gcacctgaag agttaaaaat gccagattat ataaaagaag
    13741 ctataaaggg acatgcatat tatgaaacta ataatttgga tgatgttata ggaagcttgg
    13801 atgttttata tatgactaga gttcaacaag aaagatttga agataaatca gagtatgaaa
    13861 gacttaaaaa ctattatatt ttaaataaag ctaaattaga aaaggcgtct aaagatatgt
    13921 tagtaatgca tccattacca agagtaaatg agatagacat agatgtagat agtgatgata
    13981 gagctgtata ttttaaacaa gctaaatatg gaatgtatgt tagaatggct ttaataataa
    14041 aacttttagg tataaatgaa gactaatagt tatcatataa tagtaaaaca aaggagattt
    14101 tgggatgtat aaaattctgg aaaatatata tataggcgaa gatatgtata gaatgaaagt
    14161 aaaaggtaat tttgaaggta aaatgggaca attctatatg ttaagagcat gggatactta
    14221 tccagtactt tcaagaccta taagtataca tgatatagat gaagaaggga taacttttct
    14281 ttataaagta gtaggagaag gaactcaaat tttgagtaac ctaaaggtaa atgatactat
    14341 aaaattggaa ggcccttatg gtaatggata tgctaaggtt gatggaaagg tagctctagt
    14401 aggaggagga atcggtgtag ctccactata tttggttgct aaaaatataa aaaattgtga
    14461 tgcatatctt ggctttagag aagatgttat attagaagat gaatataaac aagtatgtaa
    14521 taaagtatat acaacagtag gaaatacttt tgtaacagat ataattgatg ttgagaaata
    14581 tgattatata ttaacatgtg gtccaactcc aatgatggaa aaattagtta agatggtaga
    14641 aggtactaaa acgagaatta tggtatctct tgaaaaccat atggcttgtg gagtaggtgc
    14701 ctgtttagta tgtacgtgta aaactaatgg aggaaataaa aagacttgta aagatggtcc
    14761 agtgttctgg ggagaggacg tgatttttaa tgggtaattt aagtgtgaaa tttggaagtg
    14821 tagaatttaa aaatccagta ataatggcgt ctggaacttt tggatttgga aaagaatata
    14881 atgagattta tgatatacaa aaacttggtg gtataagtag caagggtctt actttaaata
    14941 aaaaacctgg taacaatggt atgagagttc atgagacacc atccggtatg atgaatagtg
    15001 ttgggcttga aaaccctgga gttcaaggat ttatagacta tgaattacct ttttttagta
    15061 aactagactt agtcagaata gctaatgttg gaggtgggac attagaagac tacttattag
    15121 gggttcaaat gttaaatgac aagcctatag acattattga attaaatata tcttgcccaa
    15181 atgtcaaagc tggtggaatg gcttttggta taaaaaatga agtggcaaga gaagttgttc
    15241 gtgaggtaag aaacataacg aaactgcctt tagtaataaa attatcaccc aatgcagaag
    15301 atattgtagg aatggcaaaa gtttgtgaag aagaaggtgc tgatggagtt tcattagtta
    15361 atacttttaa agcaatggca attgatataa aaaatagaag accagtattt gaaaatgtat
    15421 atgctgggtt atctggacca gctataaaac ctatagcttt gagaatggta catgaagtgt
    15481 gtaaaaatgt aaatatacct gtaatgggaa tgggcggaat aactaaagcc acagatgcta
    15541 tagagtttat aatggcagga gctacttgta tccaagttgg aactgcaaat tttatgaatc
    15601 ctagaatagg tattgaaata atagatggaa ttaatgaatt tatggataga gaaggaataa
    15661 aaagtttaga cgaaataaga ggaattatat aaggtagaat aaatagtatt ttaaagataa
    15721 aaaatataga ttaaataaac aataatttta taacattaac atggaggaaa aataatatga
    15781 gtaaagtaga tgtagtggat atattaaaga aaagtgatgc attgttggag ggacattttt
    15841 tattatcttc aggaaaacac agtaatagat acgtacaatg tgcaaaagta ttaagatttc
    15901 cagaatatgc tgctgaggta ttaagtacag ttgttgaaca aataaaagac ttagatatag
    15961 acttagtagt aggaccagct atgggtggag taatagtttc ttatgagtta ggaagacaat
    16021 taggaaaaga agctgtattt actgagagaa aagacaatac aatggagtta agaagaggat
    16081 ttgaagttaa aaaaggagca aagataataa ttgctgaaga cgttgtaact actggtaaat
    16141 caactatgga gacaaaaaga gtattagaag ccttaggtgg agaagttgta ggtgttgcat
    16201 gtatagcaga tagaactaat catgatatag gtatgcctat atatagtgct ataaaacttg
    16261 atattcaagt ttatgaatct gatgagtgtc ctttatgtaa ggaaggaaaa ttaccagttg
    16321 ttaagcctgg aagtagggag ttcaaggaat tagggatgta ataaaaactt aattatttat
    16381 agtgttactt aaaaaatgta aattttttag agtaaaaatt tgtaatataa catataactt
    16441 aaaaataaaa agcagttagc cttataaatg aaggttagct gttttttttg ttaaaaatat
    16501 atttacaaaa taagagatag tagcagtaca atataagtat aaggtaaaag tattatatat
    16561 tagggagggg taagtatgaa aaatcaatta aatgaaaatc ataaaaaggt tgtatttttt
    16621 ggagtaggtg ctgtaggagc tacttttgca gaacaatttt ttaactctaa atatgatttt
    16681 aaaattcttt gtgataatga aagaaagaaa agatatttag aagaaggatt tataataaat
    16741 ggaaaaaggt atgattttga ttatgtaact aaagatgagt ataaacaaga ggctgatttt
    16801 ataattatag gtctaaaata taataattta aaagaaaata taaaagaatt agatggatta
    16861 gttggaaaaa atacagttat aatgtctctg ctaaatggag ttgatagcga agagataata
    16921 ggagaaagat ttggaattga aaaaatggta tattcatatg ttaccaatat agatgcaaag
    16981 aaaatcaata ataatattat acacactact aatgggataa ttgtatttgg taacaaagat
    17041 aatagtgaag atagaaaaac taatataata accgaagtct ttgatgatgt aaatatagaa
    17101 tatactttat caaaagatat tcaacgagat atgtggtgga agtacatggt taatattggt
    17161 gtaaatcaaa cttcagctat acttggtgca ccttatggag tttttcagag ttctgagcat
    17221 ttaagagaat tagcaaaatc tgcaatgagg gaagttgttg ctatagcaca agcaaaagac
    17281 atatctctta cagaagatga tgtggaacat tcattacata gaatactaga acattcaaaa
    17341 gaaggaagaa catcaatgct tcaagatgtg gaagctcata gacttacaga agtagatatg
    17401 ttttctaaga atatctgcaa acttggaaaa aaatataaca tacctactcc tataaatcag
    17461 actttctttt atatgataaa agtaattgaa agtagatttt aaagggtctt tgtaagaaat
    17521 attgataatt attgatttta aattttatta atgttatata ctatttaagt tactaaatat
    17581 ttttaactct tcaatgaaaa tagttgctgc ttttgataaa gttatatctt ttggaaatat
    17641 atatgagaaa tgtctgttgt agctgcttcc aagctctatg acagacagct cattgtttag
    17701 aacaggtaag cttgtcacta gattagagac tattgtaatt cctaaattat ttctaacaga
    17761 ttctttgaca gcataattac ttccaaaaac catcatactt tttggaataa tttctttaac
    17821 acttaaaaac atatctaaat aatctctggt tccagaacca tcctccctga ctatccattt
    17881 ttggttttga agtttatcaa aactaaaatc ttttaataaa tgactcttgt atggaagtgc
    17941 taaaaccatc ttatcttcaa aaaaatattc ttgaataaat gaaggggaag agcaagtgcc
    18001 ttcaataaga ccaatgtcta aaatataatc cttaacatga gaagatacga ttgatgtatt
    18061 ttttataaaa acttctacat ctatgtcagg atacttttta gaaaataatg ctagaaaatt
    18121 tggtaaaata tactctccaa tagttaaact tgcaccaatc tttaagtgac cagttatagc
    18181 atctgaaact tggcttacat catgataagt aatatttaaa agatttaata tttctttagc
    18241 tcttttgtac aagatttgtc cactttctgt tataaaaatg gttttttgtt taatagacct
    18301 gtttataagc acaactttaa aataattttc tagatttttt atgtgtttac ttacacttgg
    18361 ttgtgataga tttaaatatt ccccagcttt agtaaagttt ttgtattcaa caacagctac
    18421 aaatgttttt aattcttcaa acaatttttt gtctccttta cctaatgaaa ttttacattt
    18481 tttatatact ttctacagat aattatacta tatataataa ccattaaaaa cattaatcat
    18541 tacgatatta tatatttatt ttacttatgg ttattacaat tatatactat tgctatgaag
    18601 aaaactatat taattataag gtaggtatat tatgttaaag aaaaaaagca gtttgacatt
    18661 aaaaactata agggaaatat taccaggact gtttgtatca gtattagtag gttatattag
    18721 tatatttatt tctattttaa ttcctaaggt aggggcagca tcaatttcta tatttttagg
    18781 tatgtttgta ggaaacctat ttttaaatca aaaggtattt caaaaaggtt ataaattttc
    18841 agagacagat ttgttatcat attcaatagt actattagga gcaacactca gtgtatctac
    18901 cttaatagac ttaaaagttt caggaatttt atttataata ttgcaaatga caataacaat
    18961 aatagctgca ctttatatag gtaaaaaact aggatttgaa gaaaacttta gatttctaat
    19021 ggcaagtgga aatgcagtat gtggttcttc tgcaatagga gcaacagttc cagtggttaa
    19081 tgcgacagat aaagaaaagg gaattgcagt aactattgta aatgttactg gtatatttct
    19141 tatgttttta cttccaataa tatctcaatt tttatatagc catgaacttg taaaaacttc
    19201 agcaatgata ggtggaactt tgcaatcaat tggtcaggtt gtagcaagtg gagcaattgt
    19261 tggagaaaat gtaaaagatt tagctactat ttttaagata gtaagagtaa tatttctagt
    19321 tgttgttgta ttagtatttg gacatatgaa aaatcaatct aatagggaaa ttcttgaaga
    19381 agaaaaggat gagataaaaa agaaaaaagt gactatacca tggtatgtaa ttggattctt
    19441 tataacttgt gcactttttt caatgaacat tataccacaa gaagtatctg tgttgtgcaa
    19501 agaaataagt aacaaactag aaataatagc tttagcagca attggattaa aggttaatgt
    19561 aaaagattta gtgaaacaag ggaaagaagt ttctttatat ggtttatttg taggtatagt
    19621 acaagttgta tcagctgtag ttttaataaa aatatttata tagcataaaa atagtatttg
    19681 taatattaat ttgattaatt aaaataatat gatactaata atttataata taaataaaaa
    19741 atgaatttcg ataagtttaa gtgggtataa acttatagaa attcattttt tatttaaggt
    19801 aaaaatttaa agtaaaatat tattttacat aataataacc ttataaattt tgaaattact
    19861 tatctttgtg ttaaaataag taaaatgcac atattagagc taagttttta agtaaataaa
    19921 caaaatgaaa atataaatat taaggagatt tggtatatga atgtttatga aaaaggtaat
    19981 ggagttatac tagaaggagt aacagatttt gacccagtac atatatttga gtgtggtcaa
    20041 tgttttagat ggcacaaaca agaagatggc tcatacactg gagttgcaaa ggggagaata
    20101 ttaaatgtca aaaaagagaa tgataaaata tatttaaata atactaattt aaaggagttt
    20161 aacagcattt ggtataatta ctttgattta ggaactgatt atacagaaat aaagaataag
    20221 ctaaaaaata tggatgaata tttaaataag gctactgaat ttggatgggg aataagaata
    20281 ttaagacaag atggatggga aatgcttata tcatttataa tttcctctaa taataggatt
    20341 cctatgatac aaagagctat agaaaattta tcaagaaaat ttggtaagta tataggtgaa
    20401 tatgaaggta atgaatacta tgcattccca acaccagaag aactaaataa ggcctcacaa
    20461 gaggaaataa gagcatgtca aacaggtttt agagataagt acataaaaag tactacacag
    20521 gcagttatag agaataatga tgaggtatct gaatatacaa atttaagtac tgaggattgt
    20581 agaaaagagc ttttaaaatt taatggagta ggaccaaaag tatgtgattg tattgcatta
    20641 tttggtatgc aaaaatatga ttcttttcca gttgatgttt gggttaagag agttatgcaa
    20701 gaattttata tagatgaaga catgagttta cctaaaatga gaacttatgg aatcgataaa
    20761 tttaaagaaa tgtctggatt tgctcaacag tacttatttt actatgcaag agaactcggt
    20821 ataggaaaat aaattaaggg ggagaaatta tgggagtaat aggaacaata tttctgttct
    20881 atttaattat atcatatcta gctggagcca taatttctgt gattatattg cttgaaaata
    20941 gagaccctgc aaaaacaatg tcttggctat tgatgtttat tatatttcca ggtgtaggtt
    21001 taatgatata tgctatttca ggtagaaata ttagaaaaag aaaattattt aagactcaaa
    21061 aactagctaa taatataaaa gaaaagaaac tatttgatac attagaaaag ataacagaaa
    21121 tagttgaatt agaaaaagaa tctataaaac aaaataagct tttaagagat gaagaggatg
    21181 gtagttatag aaaaagggta ataaatatgt tgttaaagac tggtatgttt ccctttacta
    21241 aaaataataa agtggatgta tttgtagatg gaaatgaaaa gtttaaaaga cttatagaag
    21301 atataagaga agccaaagac catattcatt tagagtactt tatcatcaaa gatagtgaaa
    21361 taggtagggt gttaaaagaa gaacttataa aaaaggctaa agaaggcatt aagataagaa
    21421 ttttatatga tgatgtagga tgctggagat tttggtttaa tagaaaattt ttccgagaaa
    21481 tgagggaagt aggtatagaa attgcagcat ttttacctac aaaatttcct ataataggag
    21541 ggaaattaaa ctatagaaat cacagaaaaa ttgttgttat tgatggaata attggatata
    21601 caggtggaat aaatatagga gatgaatatt taggcaaaaa tgataagttt ggatattgga
    21661 gagatactca tattaggatt aagggtattt ctgtatacat gcttcaaatg acatttctga
    21721 ttgattggta ttatacgaca aaagaagtat tagtgactaa aaattatttc ccaagtgtag
    21781 ggaatgttgg tgaaagtatg atacaagttg ttgcgagtgg tccagacagt gactgggaag
    21841 atattcatta tgcttatttt tctgcaatat gtcaggctag aaaaaatgtt tatatagaaa
    21901 ctccatattt tattccagat gaaagtctac taaaagctat aaagagtgct gctcttagtg
    21961 gagttgatgt gagaataata tttcctaaaa ttgcagacca caagatagta aatatagcat
    22021 cttattcata ttttgaagaa atattaagag caggtggtaa agtttattta tataataaag
    22081 gatttataca ttctaaagtc gtaataatag atgataaaat tgcttctgca ggtacagcta
    22141 atatggattt aagaagtttt atgcttaact ttgaggtaaa tgcatttatc tatgatgaag
    22201 aagttattag ggtaatgaca gatgatttct ttgaggattt aagtcactgt gaagaactta
    22261 atttggaggt atttaagaat agaaatatca tacaaaaaat caaagaatct gtagcaagat
    22321 tattttcacc aatattataa aaaattatta aaaaaggtat tgaaaattat gtgtgattgg
    22381 tatattataa taattgttag cactctatta gatagagtga taataaataa aaagtatata
    22441 tataaatcta catatataag tagtattaat ttaaaaataa ctaaaaacat aggcgaacta
    22501 gtctatactt aatattttag gaggcgtgaa taatgaaaat aagaccatta gctgacagag
    22561 tagtaattaa aaaagtagaa gcagaagaaa aaactgcaag tggaatagtt ttaccaggag
    22621 cagctaaaga gcaacctcaa atagctgaag ttgtagaagt tggaccaggt ggaatagttg
    22681 aagggaaaga aataaaaatg gaattgacag tgggagataa ggttatattc caaaaatatt
    22741 ctggaacaga agttaagata gaaggacaag aatacacaat actaagacag agtgatgtat
    22801 tagctgttat tgaataaata tagaataaat ttattaggag gggtttaaaa tggctaaaga
    22861 aattaaattt tcagaggaaa caagaagagc tttagaagct ggtgtaaata aattagcaga
    22921 tacagtaaaa gtaacattag gacctaaagg aagaaatgtt atattagata aaaaatttgg
    22981 ttctccactt ataactaatg atggagtaac tatagcaaaa gagatagaac tagaagatag
    23041 atttgaaaat atgggtgctc aattagttaa agaagtagca actaaaacta atgatgtagc
    23101 tggagatggt actacaactg ctacagtttt agcacaagct ataataagag aaggtttaaa
    23161 gaatgtaaca gcaggggcta acccaatact tttaagaaaa ggaatacaaa aagcagtaac
    23221 agtagcagta gaagaattaa aaaatcaatc aagaatagta gaaacacaag aggctatatc
    23281 tcaagttgct tctatatctg ctggtgatga agaagttgga aaattaatag cagaagctat
    23341 ggaaatagta ggtaaagatg gagttataac tgttgaagag tctcaaacta tgaatactga
    23401 attagatgct gttgaaggta tgcagtttga tagaggattt gtttctgcat atatggttac
    23461 agatgtagat aaaatggaag cagttttaaa tgacccatat atattaatta ctgataaaaa
    23521 aatatctaac atacaagaat tattaccagt tcttgaacaa atagttcaac aaggtaaaaa
    23581 gttattgata atagctgaag atgtagaagg tgaagcatta tctacattag tagtaaataa
    23641 attaagagga acatttgatg tagtagcagt taaagctcca ggctttggag atagaagaaa
    23701 agaaatgctt caagatatag caatacttac aggtgctcaa gtaatatcag aagaattagg
    23761 ttatgattta aaagaagctg atttatctat gctaggtaga gcttcatctg ttaaggtaac
    23821 taaagaaagt actacaatag tagatggttc tggagataaa aaagctatag aagatagagt
    23881 aactcaaatt aaacatcaag tagagcaaac tacttcagat tttgatagag agaaattaat
    23941 ggaaagatta gctaaacttg ctggaggagt agctgttgta aaagttggag ctgctacaga
    24001 agtagagtta aaagaaagaa agttaagaat agaagatgct cttaatgcaa ctagagcagc
    24061 tgtagaagaa ggaatagtag ctggtggagg aactgctttt gttagtgtta taccagcaat
    24121 aggaacacta attgagagtt tagaaggaga agttaaacta ggtgctcaaa tagttaaaaa
    24181 agcgttagaa gagccattaa gacaaatagc tataaatgct ggtcttgaag gtgctgtaat
    24241 tgtgcaaaat gttgttaatt ctgaagcaga aactggattt gacgcattga atgaaaaata
    24301 tgtaaatatg atagaagctg gtatagttga cccaactaaa gttagtagaa gtgctttaca
    24361 aaatgctgca tcaatagcaa gtactttctt aactactgaa gctgctgttg ctgaccttcc
    24421 tgaaaaagaa gatgtaggaa tgccaggaat gggtggcgga atgcctggta tgatgtaatt
    24481 taaaaaattt aaaatataaa ttttaatata aagaactatt tcgacttaga ttttaaactc
    24541 taagttgaaa tagttctttt gtatatatct aatttttgta tatctataaa ttcaatacac
    24601 atatttagat aatcagtatc tagtttgtaa taatatttta gagtagtatt gttatttttt
    24661 aggttatgat tttagatata tattaattaa ggtataattg ttgatagaga ataaatcaaa
    24721 tttaaaagga tgcgataata tggacttaag aaaattgctt gaagaagtta aaaatgaaaa
    24781 tattgatata gattttgcgt tagaaaagtt aaaagattta ccttatgaag accttggata
    24841 tgcaaatata gaccatcata gagaattaag aaatgggtat ccggaagtta tttattgtga
    24901 aggtaagagt gatgagcata ttttagggat tattgataaa atgaatcaaa aaagttctaa
    24961 catattgggg acaaggtgca gaaaagaaac ttttctgaaa attaaagaaa tctataatca
    25021 tgctgagtac gaagaggcgt ctaaaattct taagattcaa aatcatgata tagagaatat
    25081 aggaaaaggt aaaattgtga ttgctacagg gggaacttct gatataccag tagcagacga
    25141 agcttatcat acagctaagt ttttaggtaa tgatgttgat agaatatatg atgttggagt
    25201 ggcaggtata cataggcttt taaataaaag acataaaata gatagtgcta gagtaatagt
    25261 agccgttgca ggtatggaag gtgctcttgc aagtgtaatt ggtggactgg ttgatgttcc
    25321 agtaatagct gttccaactt ctgttggata tggagcaaat tttggaggat tagcagcatt
    25381 actagctatg ttaaatagct gtgcatctgg aatatctgtt gtaaatattg ataatggatt
    25441 tggagcagga tatttagcat caatgataaa taaactgtaa atacattgat ataaattaaa
    25501 caaaactata taataaaatt taatttctat tatattttta ctattaaaat tgaggagaca
    25561 tatatgagtg aaaaaatact ttattttgat ataataagcg gtatatcagg ggatatgacc
    25621 cttgcaagtt tattaaattt aggtgtgcct aaggaaattt ttttggagga aattcataaa
    25681 ctaagaatga gcgatgaatt taatataaac atcagctcga aaactgaaaa tggaatagta
    25741 ggtacaaaag tagaagtaat tacaaaagaa aaacatacac atagaaattt agtggatata
    25801 tttgaggtta tagataaaag cactctaaac gaaaatgtaa aatctaaagc aaaaaaaata
    25861 tttatggtaa ttggagaagc agaagcaaaa gttcatggaa ctacaataga caagatacat
    25921 tttcatgagg taggggctat agattcaatt gtagatatag taggagcttc tatattagtt
    25981 gacttacttt gtgtagataa agtttgtgct agtacagtgt ctgttggttc aggatttgta
    26041 aaatgtgaac atggaattat acctatacct gcacctgcaa ctatagaaat tttaaaaggt
    26101 gtgccaatta aattaaataa tgtaaatgga gaatgtacaa ctccaacagg agctgcaatc
    26161 ataaaagtct tgtgtgatga gtttgtagat gagcttgaat tttctccaaa gcaaattgga
    26221 tatggtatag gtcataagaa gtttgaagta ccaaatatac tcagaactgt attaggtgaa
    26281 aaaaaaaaga agaattaatt tatgaaatta gtgcaaacat tgatgatatg tcttcagaaa
    26341 tctattctta tttgtatgaa aaaatattag atgagaaagc attagatatt tatacagaga
    26401 gcatttatat gaagaagaat agaccagcaa caaaagtatc aattttatgt aatgaatcag
    26461 atttagaaaa atttattaaa atattacttt tagaaactag tacttttgga gtgagatata
    26521 atgcatataa tagagaaatt ctagagagaa aatttgtaaa attagataca aaatatggag
    26581 tggttacagt aaaactaggg tattataaag gaaaactagt caaggctact ccagaatata
    26641 atgaatgtaa aactatagca aaaaatgaag atattcctat aaaaaaagtg tttgctgata
    26701 taaattttag aataaaagaa gaatttagtg aaaacttatt gacataagta ataaattttg
    26761 ataaattaat attcagcctt attaaataaa agtaaacaga aaaaaatata taaaatataa
    26821 actcactcgt atatgctcag aaatatggtc tgagagtctc taccaagata ccgtaaatat
    26881 cttgactatg agtgaaatta ttataccaaa agacctagtt aagtaaatgt gtaggctgag
    26941 ttaaactccc tatgtgttgg ctataagata aaggtatttt ggaagcctgt atagtaattt
    27001 cacttttgct agaaattatg gaagttacta tacaggtttt ttgtgtaaaa agatttaata
    27061 taatttagac tgtattgtgt taaaataaat atataaaaac tatattatac aaggagagat
    27121 gtaaatttat gaaacatgaa ttagtacttg taatagattt tggaggacaa tataatcagt
    27181 tgatagctag aagagttaga gaaaacaacg tatattgtga aatacttcca tgtactgcaa
    27241 gcatagaaag aataaaagaa aaaaatccta aagggataat atttacaggc ggtccaaata
    27301 gtgcgtattt agaagattct ccaacaatat caaaagaaat atttgaacta ggagtaccaa
    27361 tacttggaat atgctatggt attcaaataa tgtctcatgt attgggtgga gttgtaagaa
    27421 aaggtaataa acaagaaaaa gaatatggaa aaacagctat aacttatgga aaatcatcat
    27481 tatttgaagg aataactaca aatagtgtat ggatgagtca tacagattta atagaaaaag
    27541 ttccagaagg atttactata gttgcaaata ctaatgattg tccagtagca gctatggaaa
    27601 atgtagaacg aaacctatat ggagttcaat tccatccaga ggtagaacac tgtttggaag
    27661 gggacaagat acttacaaac ttcttatata atatttgtaa agtaaaaggt gactggacta
    27721 cagattcatt catagaagat aaaataaaag aattaaaaga aaaaataggc gataaaaaag
    27781 ctttatgtgc tttaagtgga ggagttgatt cttctgttgc tgcagttctt attcataaag
    27841 caattgggga caacttaact tgtatatttg ttgaccatgg tttacttaga aaaaatgaag
    27901 gaaatgatgt agaaagaata tttagagaga aatttgatat aaatttaata agagttaatg
    27961 cagaagatag attcttatct aaattaaaag gagtttctga accagaagct aagagaaaaa
    28021 taataggtga agaatttata agagtatttg aagaagaatc aaataaatta ggaaaaatgg
    28081 atttcttagt acaaggaaca atctatccag atgttataga aagtggacat ggaaatgctg
    28141 ctactataaa gtctcatcat aatgttggtg gaatcccaga agatgtagac ttccaagaaa
    28201 ttgtagaacc attaagagag ttatttaagg atgaagttag aaaaatagga ttagagcttg
    28261 gtatagaaga aggattaata tttagacatc ctttcccagg accaggtcta ggaataagag
    28321 ttataggtga tgtaactaaa gaaaaatgtg atatattaag agaagctgat gctgtttata
    28381 tggacgagct tagaaaagca ggtttataca gagaaatatg gcaagctttt gcaacattac
    28441 cagatgttaa gactgttgga gttatgggtg atgaaagaac atatgcttat ttagttggtt
    28501 taagagcagt tacttcttct gatggaatga cttctgactg gtataagatg ccttatgatg
    28561 tattagaaag aatttctaat agaataataa atgaagttga tggagtaaat agagtagttt
    28621 atgatataac ttctaaacct cctggaacta ttgaatggga ataaaatata gaatgccaaa
    28681 aatccacata aatacaggat ttgaggtctc tcttacttct atgttatact ggtttgatac
    28741 tatcaatatt tgccagtatc ttatggatta ataagcaaaa aacatcctgc tgaatgataa
    28801 attcagcggg gtgtttttat tttgttttag aactcctttg ctctgaaact cgaagtattt
    28861 aacactgatg attttgttat cttttagttc cttttgtgtc atgctctgaa agacaaattg
    28921 atacaataaa aaagtaattt cttaatcatt gatggagata tgtgtttatg ttactcttga
    28981 tggtaatttg agtaatattg ttcatagctt tttatattca cttaattaga aaaccatttg
    29041 gtataatcat aaataaactt gatgatataa tagaaaagat ttataattat tagaagtata
    29101 taaaagtatt ttatgtctat gaaatgtatg tctataaaac atatagatga cttttgtata
    29161 ttaatgtatg aatattgata ttatagaaga attgagttgt caatgatact aaatcaaata
    29221 tttatagaag ctggagggat tatgagaaat aaaataatta taggaattac tttttcactt
    29281 gtattactaa tgtctggatg tgcttcaaaa caaaccgcag aaataaacgt attagcaact
    29341 actgatttac atggagttat accttatgag ttaacttctt atgtaaaaga agaaagaaaa
    29401 aaagataaaa atctaacttt agtagatgca ggagattttt ttgatagtgg agaagtgttt
    29461 ggttctagca tggatagtta ttttagtgct agatataaag atggtgataa taacactgaa
    29521 agttatattg agacgcctat ttcaaaagat atgaaagagg taggttatga tgttgcagtt
    29581 cttgggaatc atgaatttat atccaacaat aaattttatc tagataatat gatatctgat
    29641 tttgagaagc aaaaaataaa tattttatcg gcaaatacgt ataaaaaaaa tggagatagt
    29701 tatacaaaac catacataat aaaaaatatt aatacacctg aaggaaacgt aaaactagga
    29761 attttagggc ttactataaa agaagttggt gagaggaaac agtggaaaga tggaaaactt
    29821 gttgactcta agtctttaga actaaaagac caagagggtt acaatggtga attgtatatg
    29881 aatgatttag tagaagatgc taaaaagtgg gttggtataa tggaaaaaga gaagcctgac
    29941 attatagttg ctgtggcaca tacaggtgag aaacctaaga aacctaaaaa cccaggaaat
    30001 agaatacaag acttagctca acaagttgat ggaattgatg ctatagttgc aggtcataat
    30061 catgttcaaa ttgagcaaca tgattataaa aataaatcag gagaaaatgt tatagttact
    30121 gagccaggaa agcatgggga atgtatttct aagattaatt ttaaattaga aaaaaacaat
    30181 gataaatgga atatagttga caaatctagt aaattaatta aatttgaaaa aaatcaagaa
    30241 agtgataaat ttggagaatt tatttcttca attaataaag taagtaaaga taagaaagaa
    30301 ataagattat cagaagtaaa gactcctgaa tgggataaaa tatatgtttt taaatctgga
    30361 acatctgcag atgatatata taaaacaata ggatataaat ggagaaatat atcatctcca
    30421 aagggtgatg acatggtgca gattgttgta atgaataata ataagcctgt tagctatgta
    30481 tatggtgaag ctgaagttat gccaattaat ataaaatgtg atgaatcaga gtataaagat
    30541 aatgttgtaa ctatttatcc taataaaaat gataaattta aaattgaaaa aggtaaacaa
    30601 gaatatagta ttaatttaaa acatatacaa tagaaacttt tatcttattt aagtttagat
    30661 tgtacttaaa ataaagtgtg tattatttta aaaattgatg ataattaaaa tcacaacatt
    30721 aaagaatagt acacactttt tatgctaaaa aatagaatgt taaaaaatta aacctttaaa
    30781 ttaaagtaca ttgcttatat tatccaatat tattttcaaa ggttgtgtaa tatttttatc
    30841 cttatgtaat attgctaaca ttttattttc catatctatc ccatctaatt caatcaatcg
    30901 caatttttta tgtaagagtg aatctaataa taaattttct ggcaaaatgg ttatacctaa
    30961 ccctgcttgt gcagctttaa taagtgcaaa agaattaaca ctttcccaaa caggataagc
    31021 tttttggttt gaaagagata atacactgtc caaagtattg cgaatggcac ttccttgctc
    31081 acgtaacaat aacggaaacg tgcataactg tcgtagagat aaggcttgtt tagtattggt
    31141 ggtggttatg caatcatact tttaatccaa gagaaagtag taaatcaaac tggatggata
    31201 agtgagaaaa tgtttacaga tattataact atttcacaaa tgacaccagg gccactggct
    31261 gttaatactt ctacttttgt tggacttcaa attggagggt tttggggagc agttgcagcc
    31321 actttaggtt gtgtattatg tggtgtagta atatctctat ttttatataa tttttttcaa
    31381 aagcatcaga actctactta tatatttgaa atattaaatg gtctaaaatc agcttcatta
    31441 ggtttaatta tctcagctgc ggctacaatt ataatccttg cattatttgg gacgaataaa
    31501 gtgtacttca attctatatt tgagatgcta aactggactg cactaataat cttttttgtt
    31561 acactactga tagtacgtaa gtggaaaatt aatcctattg tgattataat aataagtggt
    31621 atactgggaa tattttttta tttataagtt tttggtaagt agaggatatc tattttttta
    31681 tatcttaaat ccaaatagcc aataaagtaa ggccatttgg atttttaata aattctgaaa
    31741 agataatcag aaacccatat aacatttaaa ttatatcact ggttttctac aaaccaatca
    31801 taagcttctt ggcaaaattt ctcactatct atatagtaac tattatgaac attattaatt
    31861 tcaacaagtt tctttttaca ttttagtgaa ttataatagc tatcaaaata ttgggtttta
    31921 aaatatctat catgaagtgt tttaaagaca agagtaggta tgttgactcc atcttttatc
    31981 ttactatctt ttgcaataaa gcattctaac attgtattaa ttatctttgt actatttttc
    32041 caaatatgag gatttttctt ttcaaatatt ttataaaatt cattatcata gacttcatta
    32101 taacctttat atttaggtgg ttttactgtt atgtgaaaat taggaagaaa cttaaagaaa
    32161 aagtttaata atttaacagt gagataggta tagcctactc ctgttgtgtt tttataatca
    32221 acacaagcaa actgagcaaa actttttaac ttattgtctg aacatgcata atattgtgca
    32281 aacataccac caattcccgt attcccatat agatgtatgt tggaagaata atttttttct
    32341 atgtatgtaa tgacagaatc taagtctttt acaattgaat tgcgagaaaa atctttttca
    32401 tatccatcac ttttccctgt tcctgaaaaa tcaaaagcaa atatatttac attatgtttg
    32461 gcaagtggtt catatgcttt ttgcatctca agcgtagtga caataggagt atgtgtaaca
    32521 acaatattca tggatgattt tctggtaata aatgagttac aaaaaatcat tgtatctcca
    32581 tttggtattt ttatatgttt ccaactcatt tgattttcct ttctctatta taaaatataa
    32641 taatttttat gttctgtttt atatattatt catattttat cacagataat atactaaagt
    32701 taattttttt attgtacacc tattataata attcttctat aatataagac attctataat
    32761 tttatattta agttcatgtg tatttaattg tttttatata aaatgtccag taatactatt
    32821 tggatttatt tttatttcat caggagttcc agaagcaaca atttcccctc catttattcc
    32881 actgtctgga cctatatcga taatccaatc agaagcacgt ataacctctg tattgtgttc
    32941 aataattaaa agtgaatgct gttcatttac aagttttcta aaagtaatta acaaattttc
    33001 tatatcttta aaatgaagtc cagtagtagg ctcatccaat atgtacagca ttcgtttttt
    33061 agtttttgtc cctaattctt ttgctaattt tatcctttga gattctcctc cacttaatcc
    33121 taatgtattt tgtccaagct ttagatatcc aagtcccact tcatctaata aggagagttt
    33181 tctatatata tcagagcaat cgctaaaaaa tgtttttgct tctgctactt ccatgtcaag
    33241 tatatctgca attgtctttc ccttgtattg tatagacagt atttcttttt gatatctctt
    33301 accttgacat tcactacaag ttacccattg gtctgccata tattgcagaa taatttttgt
    33361 tttacctaat ccttgacagt ttgggcattg cccctttttg ctattaaagc taaaatagct
    33421 ttcatccaat aatttttgtt ttgcttgtgg agtttcagca aatagagaac gaatttcatc
    33481 aaaaatattg atatatgtag caggtgtaga acggctagat tttccaatag gtgtttggtc
    33541 cattaagata aaatcatcaa tagcatcaaa tccagtaaat tcagtataat ttttatctgg
    33601 gatagatttt tgtttcaatt tttcttctag agcaggaagt aaactgtgaa atacaagaga
    33661 gctcttacca gaaccactaa cacctgtaat agagcacata cagtttaagg gaatttctac
    33721 atcaatattt tttaagttat ttgcatggca tccttttaag cttaaccatt tacttggtat
    33781 aattttattt tgtgaactta ttttattgta tgtgcttaaa tatttacctg ttatagagtt
    33841 tggatttttt ataatttctt gggttgtacc ttctgcaata atgaaccccc cttttgtacc
    33901 agcacttggg cctacatcaa taatataatc agcagacagt attgtatctt tatcgtgttc
    33961 cacaactata acagtattac ccttatctct tagctcttct atcattttga tgattaaatt
    34021 atggtctctt gggtgtaaac caatagatgg ttcatctaaa atgtaggtta agcctactaa
    34081 ttcacttcct agctgacttg ataatcgtac tctctgcagt tctcctccag ataaagttgg
    34141 agctgttcta tctgttgata tatagctaag acctaccttt aatagataac taactcgaat
    34201 ttttaattct gttaagatat catttaccaa agagagctca tttttttgaa gttgatttgg
    34261 taattcacac aaccaattcc ataactgttc tatcgtcatt tttgttagtt cagggaagcg
    34321 ataacctttg attgtagtga atctagcctc aatgcacagc aattcgccac cacatgtatt
    34381 gcaaggaatt ttgttcatat attgatgcaa ggtattattt tcagaactag attctctaaa
    34441 aagtctttga atgtggttga ttgctcctga agcaggtctt ctaatctttg attctcgtcc
    34501 ctttgattca aagctaaatt catatatctt atcgtctgta ccatataaga ttgcatctat
    34561 aaaatctctt ggtaattcat tccaaggtat atctaggtct atatttttat cttttgctat
    34621 tgcatataat tcacctacca tccaattacc atttggcttt ttccccttca attttccaaa
    34681 atagtaagta gctccatcta atattgaaat agttgggtct tttacaatca attgaggatt
    34741 aacttgatag gtaaaaccta atccattaca atcgtgacaa gctccagtat gggagttagc
    34801 attgaaggtt gaagtattta tcttagaaaa aatagtttga caatgtggac aataattttt
    34861 tgtattattc tttttttcta atggaatttt acaatatgga cagtaacttt ttccgattga
    34921 tacaaataac attcttaaat aatcccaaat gcttgtaaga gtaccaacag tagagcgagg
    34981 attttggctt ccttttttct gttcgatagc tatagtaggg gttaatccct gaagtaaatc
    35041 aaattccggt ttttgaagct gttctgaaag ttttgctcca ttacttagat tttcaaaata
    35101 acgtcttttt ccttcttcat aaatcgtatc gaaagctaaa gaagattttc cactaccact
    35161 tacacctgta ataacagtta tttgattttt tggaattgta acctcaacat cctttaaatt
    35221 gttttgcctt cctcctttta aatagataga gcttttatta tgacaaagtg aatttttaat
    35281 tgatttattt attgattttg taaatttgaa tttcttttcg agttcaagag gattgcctaa
    35341 ttcttttatt tccgaaatgc ctaatatttt taactgagaa aagattgatt tcaaataatc
    35401 ttcaggcaat ttttctatta acaaattaag gtcttctatt tttatatttc cgcttgataa
    35461 tatttctgcc atttcatcta ataaattttt ttctgctctt gttaattgat tgtatttttt
    35521 ggaacttaat tctgtccata aatcccatgg aagcaattct aaaccaatca tattattaaa
    35581 gtcatgtaga agattaccct ttacagaatg ccaacctttc caatttttat tatatccaaa
    35641 gtcatcttga tgagcatgtc cttgccgaca tcttatccag gcttctgaac cagttaaaaa
    35701 accatctgtt tttccgacat catgagtgtc aaaattaatt tgaaaataat ttttttgtat
    35761 gtcatctatt tgtgcatcta ttcttatcca tctctttgtt agagtatcat agtattctac
    35821 aagccagtgg tcttcataag taagctcact ttcaaaataa ttagcaaaac ctgctctcat
    35881 tctagcctca aaacctcgat atcttaggag cgatacaaga agtaaagaat aatctctgca
    35941 cattccaaca agcctttgtt ttgggtttct tggaatagtt agtgggttag aatttatttc
    36001 aaatattctt tttaacattt gaggaattgt acgaagcaat tcctcatcag attgttgttt
    36061 attaaaggat ataccatata attttccttg gtctccatga attactaggc tttgaaccac
    36121 ttttaataac tcaattggag tctttggtaa atcttgatat ttattttcaa attttttagg
    36181 atttgtgatg atgctttgat ttttatagtt atttattttc atttatattt cacctctcta
    36241 aatggatgat ataccctgac atggtgtcat atgcaagggc aatttaaatt ttttggccaa
    36301 aatttaaaaa taaataaaaa atgatacagt gataaattgt aatacaaata tagtttcaaa
    36361 ttgcatataa tataattatt taataaaacc attatctatg gaatgttaat gaaatgttaa
    36421 gatggcattc ttgaatatta agaaatagaa tactataata tagtgtgaag gatttttagt
    36481 aaaaaaattt gtaagtgtta atatacatta aaaaatagat ttataatttg gaggaaaaaa
    36541 atgaaattat caaggtataa gaaaaatagt ttagttctgt ctataatttt atgttcactt
    36601 attatttcaa tccttgtaat tactgttttt aataccgtaa aactacacaa tatatctaat
    36661 aaacaatcta aaaactattt aaatgatgta tctacacaga ttgtaatgaa tgttgattct
    36721 aaaatcaaat ttattttatc cgacttaaga ataatggcag attttattaa gcaatatgaa
    36781 ggtgatagtc gatatgagta tttatctaaa agaaagacta gttataagta ttatgatgtt
    36841 ggaataatag atttagaagg taaagctgag tttttaagtg gaaaaaagtt tgacttaaag
    36901 aatactgttt cctataaaaa aggaattgaa ggtaaagaat actcagaagt aatagagtca
    36961 atgggttttg ttctttatag tgtacctata tttaatgata atcatgaagt atctagtatt
    37021 ttaataggtg tatccaataa agaaagtatg aatgagattc ttaatataga taactttaat
    37081 ggaaaaggaa ctattgaaat tattaatagt aagggaaaac ctcttttcat aggaaaaaat
    37141 actgaattaa taaaggacct tagccataaa tataagagtt ctggagatga accttgggca
    37201 caaaagatgc ttgaggattt taaaaatgat aaatcaggga atattacaat aacctcatca
    37261 aaaggtgtac aatgcttatt aacttatcat ccaattacaa gaggtttaaa tgattggcat
    37321 tttcttctca tagtacctga agatgctgta cttggtgaac ttaataaatt aaactctttt
    37381 acaataacaa tgacatttat aattacatgt attattggaa ttataacatt aattttatat
    37441 attataagaa gaaaatatgt aaatcaaata gaaaatattg catatttaga tagtataaca
    37501 aatggtataa attcaactaa gtttagtatg ctagtcaaac ctttaatttc agaatcacct
    37561 gatagtactt atatgatgat agcaatgaat ataaaagatt ttaagctaat aaatgattgt
    37621 ttcggtagtg aaaagggaaa tatgacacta aaacatcttt acaatatttt gtataaaaac
    37681 attgatgaaa atgaagagtt tgtgtgccgt catgatgcag accttttcta tttgcttatt
    37741 aagaatagac cagtgttaga agccctggag ttattatata aaatagaagc tgacacaaat
    37801 tattttaata aaaataggga aaatccatac tttttaagac tatcagtagg tatttacaca
    37861 attgaaaatc ataatgaaga tttaattaca atacaagacc atgcaaatac agcaagaaaa
    37921 agctttaata aaactcatca aagtgatttt tctttttata gtgatataga aagaaaacga
    37981 ttaatatctg aaaaggaaat ttcaaattta atggaaaaag cccttgaaaa taaagaattt
    38041 tttatgtgct tacaacctaa aattgatata aaaagtggaa aaattagtgg agcagagtct
    38101 cttgtgcgtt ggaaaaattc agaaaagggc ataatttatc caagtgattt tattcctctt
    38161 ttcgaaaaaa gtggatttat atgtaagtta gatttatata tactagaaga aacatgcaaa
    38221 ttgatttcta agtggataaa agaaggtaaa gaagtgttga gaatttctgt taatgtatca
    38281 cgtcaacatc tgaatgataa attgttttta gaaaaatata agcatatatg caataaatac
    38341 aatgtaccaa catgtctgat agatttagaa cttactgaat ctattttctt agaaaatcca
    38401 gaagctatag atataataga ggatattcat tctaatggat ttaaatgttc tatagacgat
    38461 tttggatttg ggtactcatc attagggata ttaaaggatt ttaaagttga tataattaag
    38521 ttagaccgtt cattctttgt tagcaaaaat aatatagacc gtggtaaggt agttattaag
    38581 tctattattg aacttagcaa aaggctaggt atgaaagtaa ctgctgaagg aattgaagaa
    38641 ttagaacaag ttgagttcct aaaaaatgtg ggatgtgatt atattcaagg ttatgttttt
    38701 tcaaaaccat tattaatttc tgactttgaa aattttgcat atgaaaataa taaaattaaa
    38761 aatatccatt attaaatgta caagataaaa aatttaggca agttatataa ttaaaaatat
    38821 aacttgcctt ttttatttat atatttaata aaaatatatt tgcagatttt gtaaaaataa
    38881 ttttcaaatg ttgtaagttg tggctaaagc tttattgtat tattctgaat atagaaagat
    38941 agttactgtc ttagataaaa actgaaacat acataataat ataaaatttg ctatattaat
    39001 aactctaatt attttggaag gagggaaaat atgataagtg ggagaatgtt actcatcatg
    39061 aattttctaa aaggaaaaaa taagacaagt tacaaggata ttagcaaaca acttagtata
    39121 gaagaaagaa aagttagata tgatataaat aatttaaata ttgttttgcg ttcattaaac
    39181 aagtcagtaa tacaaaagat gaacaaaggt gtgttaatta ttcctgatga cttccaagta
    39241 gtatgtataa ataataacga atatgtattt tccttaactg agcgagtatc tattatgaaa
    39301 attatatcaa tgtttaaaat agataatcta aacttagaaa aattaagtaa agaatttcag
    39361 gtttctagaa gttcaattaa aaatgacctt aatgttttgt caagtgaatt agaaagaaac
    39421 caaataacta ttacttatga taaatctttt aaaataagtg gagaagaaga cattatatat
    39481 aagcaaagat tgaacatatt aaaatcgtat tcttatcttt ttggtaagga aaaatctgat
    39541 ttaagtgtat ttgagaagta tttagtgaat gtaattgaaa aaatattcaa agggatttat
    39601 ttgattgata tatataattg gtctatgaat ttattattgc aaatgggatg gactttaaat
    39661 gatgaatctt ttacatggta tgtgtcaaac attttccttt ttacatggta tatacattca
    39721 gatgaaaaac atcctttaga atctgcttca ttaagtgagc cgtgttttga aaataagttt
    39781 gaccaagaat ttgaaacaat tattcaaaaa aagttgacta aggaacaaat ttttctgtta
    39841 aagagttttg tattttttac aaataaatat gcaagtttaa acgaggatat ggatttgatt
    39901 agtacagaaa ttatagttca agacctaatt tacaatatgt cagaaatatt agagattagc
    39961 tttaaagaag atatgattct ttataaagga ttactcaatc atattgcccc attgatagag
    40021 agaatcaagc agaatataca gatttatgag gaattacttc atgtaatacc agataaatat
    40081 caatatgtac ttgaagctac taaggtttct attcaaaaaa acagagtttt aagtcaaata
    40141 aagaatgaaa atgaaacaat acttttggca atacatttct taggaagtat acatagaaat
    40201 gagaggaata actacaaaaa tatattgtta gtttgtggat ttggatatgg tgtaattgct
    40261 atgttaaagg atagtttagt aaatgattac cagataaata tagtagagag tattccatca
    40321 tataagttac aagattataa agactgggaa agtattgact tcattataac aacatcaaag
    40381 atagtaatag acttgcctaa aacaattatt gagataaatc catttttaca agaagaggat
    40441 tatttgaagt ttgaagaaaa agggataaaa agaaagaatg tcttaactaa ttatatgtca
    40501 ataaaaaaaa ggttggactt tttagaagaa gatgcccaaa aaagagtatt aagtattata
    40561 tgtcaagaac ttggatacag tgatgagaga atattattta gacaacttaa tttaagtgat
    40621 ttaataggga ttgataccat aaggataatt gatggagata tgaaatggga agatgctgta
    40681 aaatttagtt caaacatact tgctaattgt gattttgtat cagcacaata tgctgagaat
    40741 attatagata ttttaaaaaa tgtgggtttt tatgctgtaa aagataacga atttgcttta
    40801 ctacatggca atgatagttc attagtcaaa gtaagttcga taagtttatt aatatgtaaa
    40861 gaagcagtta gttttggaga taaaaaggtt aaaatcatat tttgcctagc aagcagagat
    40921 aaaaaagagc aaattcctgc aataattaat ctaactaaaa tggtttataa gactaatttt
    40981 attgctatgt tagaaagtgc aaagacaaaa gaagaagcag aagttttaat tcataaatat
    41041 gagaaggagg tcactgtatg aataaattag ttaatgtaaa atgttcattt aaaatacatg
    41101 ttaattcaaa agaagaagcc attatatcgt tagttgatgt agtgtcaaaa gaaggatatt
    41161 taatagataa aaatcaattt ttaaaagatg ttttaaagag agaagaaaca ttgtcgactt
    41221 atattggaca tggtataggt cttcctcata gtcaaagtgt aggtgttaaa aactcttgta
    41281 ttacaatagg aaagcttgat actcctattg aatggacaga agaaggagaa aaagtggatt
    41341 taatattttt gatatcagta acaaaagaca atgaaaataa tcttcattta aagattttat
    41401 caaaattagc acgtttattg atgcatgaaa gttttagaaa tcaaattaga tgttcagatg
    41461 aacaaactgt ttataatctt ataaaagaaa aaatagagga ggaagattaa aatgagaatt
    41521 ttaaagtttt tgcaaaaaca tcttatgaca gcaacatctt atatgattcc atttgtggtt
    41581 gcaggaggaa tattatttgc tttatcagta actttaagtg gacaagctgc tgttcctgaa
    41641 acaggctggt tggctaaact gaatcaaatt ggagctgctg gcttggcttt attcattcct
    41701 atattaggtg gatatatagc atttagtatg gcagataaac caggattagc tccaggtatg
    41761 ataggtgcat atttagcaaa agaagtaaat gcaggtttta taggtggtat catagcagga
    41821 tttatagcag gatttgttgt tttacaatta aagaaaatta aattagctcc tacaatgaga
    41881 actttaggtt ctatatttat ttatccttta gttggtacat tgattacagg tggtataatt
    41941 gtatttttaa ttggagaacc tatagcttct tttatgacat ggatgacaaa ctggttaaat
    42001 ggaatgtctg gtgtttctaa aattccttta ggtggaatct taggaggaat gatagcctca
    42061 gatatgggtg gccctataaa taaggttgct gctacttttg cccaaactca agttgacaca
    42121 cttccatatt taatgggtgg agttggcgtt gcaatttgta tacctccaat tggtttagga
    42181 ttagctacat tattgtttcc aaaaaaattt agcaaggaag aaagagattc aggtaaagct
    42241 tcactattaa tgggatgtgt tggaattaca gaaggagcta ttccatttgc aacatcagat
    42301 ccagttagag taataccttg tattatggct gggtcgattg taggaaatat tatggctttt
    42361 ttattaggat gtttaaatca tgcaccctgg ggaggtctga tagtgttacc tgtagttgac
    42421 aataggttgg ggtatattgc atcagtatta actggagcag tagttgttgc ggtattaatg
    42481 aaattagtaa aaaaagacgt gaaagaagat gaagaaattg aagaagattt agatgaatca
    42541 attgaattaa tatttgaaga attataaata taaatggaag gaatttgtat aaaaaagtaa
    42601 atataaaact ataaatttaa aggagataag aatatgaaaa ttgtagcagt aacatcttgt
    42661 cctagtggag tagcacatac ttacatgtcg gctgaatcac ttgaattatc agccaaaaaa
    42721 tttggtatag aaataaaggt tgagactcaa ggaagctctg gaatagataa tgaattgtca
    42781 cttaaagata ttgatgaagc aacttgtgta atattgacta atgatgttga gattagaaat
    42841 atggaacgtt ttaaaggaaa gaaggtagta agaatgagtg tatctgagat aattaagaaa
    42901 tctgatgcat taattaagaa aataaaagat acttttcaat aaaaattgat ttattttaca
    42961 tataactcaa agatgttttt aaagaaattt ttattaaaag catctttgaa aatatcttaa
    43021 aaatagaata aaaggagata tagtcatgaa aaagttacca ataaagactg tcgtgcaaag
    43081 attactaaac ttacaagaag aggggaaaag tgcaactctt ttaggaattg gtcctatgtc
    43141 accaaacttg ttacaagcaa gttttgaact agcaaaagat gatgattttc ctttaatgtt
    43201 tatcgcaagt agaaatcaag tagatgcaga tgaactaggt ggcggttatg taaatggttg
    43261 gaatcaggaa acttttacaa aagatattaa gaaggttgcc gacaaagttg gatttgatgg
    43321 tttatattat ttatgtagag accatggtgg gccatggcaa agagataaag agagaaatga
    43381 ccatttgcct gttgatgaag caatggagtt aggaaaaaaa tcttatttag cggacattga
    43441 agcaggtttt gacctattga tgattgaccc tacaaaagac ccatttgaaa ttggaaaagt
    43501 aattccacta gatgtagtac tagaaagaac tgtagaatta attgagtatt gtgaaaacga
    43561 gagaaaaaga cttaatctac cagacatagg ttatgaggtt ggtacagagg aaaccaatgg
    43621 aggtctgact tcaactgaga cgtatgaaac atttataact agactaaagg ttgaactcga
    43681 taatagaggt ttgccaatgc caacatttat agttggtcaa actggtactt taactagaaa
    43741 gacagaacaa gtaggaactt ttaattttag aaatgcttat gatttagctc aaatggctaa
    43801 gaaatatgga gttggtttaa aagagcataa tggcgattac ttagatgatg taactttatt
    43861 agaacatata ccatctcaaa ttattgctac aaatgtagct cctcaatatg gtactgaaga
    43921 aactagggca tatttaaaat tagcagaagt tgagtgtaaa ttagaaaaag agggcttagt
    43981 tgagaaaact tctaatatca gacatgttct tcttgttaat gcaattgaat gtggacgttg
    44041 gagaaaatgg gtagtaggag aacaaaaaaa tctaactact gaagagatat ttaaggatga
    44101 agttttatct aatgaaatac tagatattgc aggacattat acatttaata atgatgatgt
    44161 taaaaaagaa atagaagtgc tttatgataa tttaagtaag aataatattg atggtcaaag
    44221 atttgttgta gaccatatta aacgtccttt gcgtgattat gcagaatgct ataacttaaa
    44281 aggtgttact acgaggattt taaataaata ataggactta tagtggtgag tattgttata
    44341 ctaggtattt atagttataa ttatacttac cactattaaa tttaggagga ctaatatttt
    44401 gattaattta atttttgatg tagatgatac tttatataat caattaacgc ctttttacac
    44461 tgcatataat aaagtgtttt cttcaataaa ggatatttct attgaagatt tatatatgag
    44521 tagtagaaag tatagtgatg aggtatttca tatgactgaa aatggagaaa tgccaatcaa
    44581 agaaatgcat atttatagga taatgaaagc ttttgaggag ttgggaaatt ctattacaga
    44641 aaaagatgca caaagttttc aagatgagta tatatatcaa caatcacaaa ttacactaat
    44701 accagaagtt gagaggattt taaatttttc taaagaaaga aatattaatc taggaattat
    44761 aactaatggc ccatcaaacc atcaaagaat gaagttaaag cagttaaata ttgaaaactg
    44821 ggttgataaa agtaatattt ttatttctag tgaggttgga ttttctaagc cagatactaa
    44881 tatttttaga gtagctgaga atgttatgaa tttagataga gaaaatacat attatgtggg
    44941 tgattcttat agaaatgatg tattaggtgc taaaaaagct ggttggaaga gtatatggtt
    45001 gaaccataga ggacatgagg tagaagagtt gttttacaaa cctgattttg taattttgga
    45061 acataaggat ttgatatctt tatttataaa aatatgtagt tataaaaata tgtagtaaaa
    45121 aaatgttaat aatagaattg tataaaataa ttctataaaa ttttatatct aattttagat
    45181 tatctgcata aacactatac ttttaagcat tttgaaatat aagaaaacac tcattttacc
    45241 acgaatttac cacgattgaa tgagccttga tatgacctat aaagtaacac cagaaagaca
    45301 tcattatacc taaatggtgt ctttttaaaa agcagtcaat cataagacta actgctttgt
    45361 gtgtacagat ataaattttt gaaaacttat aaactcttta aaattgttgc taaattttct
    45421 ttatgccatg aagcaccaac cattgttttg ttcactttaa aagctaatgt tccttgtgat
    45481 gtactaattt ctaattcata agacattaac tttttattga atttaacact tgttacttct
    45541 tcttttggta cgaaaaggaa tgcttcattt tctaatttgc ctgttacatt atttaaaccg
    45601 ataacagcaa taccgctatc gccaaaattt attgcgtaat attgcattga aagagcagtt
    45661 gcaactggac cgagagcata tgtaaactta ctagggaaga taacaccaat aatagatgta
    45721 ttctccttaa
//
LOCUS       DBGJFU010000015        45347 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.015, whole genome shotgun sequence.
ACCESSION   DBGJFU010000015 DBGJFU010000000
VERSION     DBGJFU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.015"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(15..1943)
                     /locus_tag="ACOIAS_000839"
     CDS             complement(15..1943)
                     /locus_tag="ACOIAS_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2700558.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            2177..2629
                     /locus_tag="ACOIAS_000840"
     CDS             2177..2629
                     /locus_tag="ACOIAS_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2700559.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDASHLVKYITNKMKEE"
     gene            2632..2940
                     /locus_tag="ACOIAS_000841"
     CDS             2632..2940
                     /locus_tag="ACOIAS_000841"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGZ2700560.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3054..4160
                     /locus_tag="ACOIAS_000842"
     CDS             3054..4160
                     /locus_tag="ACOIAS_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGZ2700561.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4235..6913
                     /gene="mngB"
                     /locus_tag="ACOIAS_000843"
     CDS             4235..6913
                     /gene="mngB"
                     /locus_tag="ACOIAS_000843"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGZ2700562.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7230..9566)
                     /locus_tag="ACOIAS_000844"
     CDS             complement(7230..9566)
                     /locus_tag="ACOIAS_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2700563.1"
                     /translation="MLDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGG
                     AENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDT
                     YDGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVL
                     DTTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSY
                     GVNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYG
                     YGNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTL
                     SRIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNL
                     TYWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINL
                     KEKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQL
                     LDVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNV
                     SHDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSG
                     DVYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENL
                     IINISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTE
                     GSGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9538..10254)
                     /locus_tag="ACOIAS_000845"
     CDS             complement(9538..10254)
                     /locus_tag="ACOIAS_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700564.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(10669..11325)
                     /locus_tag="ACOIAS_000846"
     CDS             complement(10669..11325)
                     /locus_tag="ACOIAS_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2700565.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(11380..12009)
                     /locus_tag="ACOIAS_000847"
     CDS             complement(11380..12009)
                     /locus_tag="ACOIAS_000847"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2700566.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(12140..12781)
                     /locus_tag="ACOIAS_000848"
     CDS             complement(12140..12781)
                     /locus_tag="ACOIAS_000848"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGZ2700567.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(12790..13461)
                     /gene="rpe"
                     /locus_tag="ACOIAS_000849"
     CDS             complement(12790..13461)
                     /gene="rpe"
                     /locus_tag="ACOIAS_000849"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGZ2700568.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(13466..14350)
                     /gene="rsgA"
                     /locus_tag="ACOIAS_000850"
     CDS             complement(13466..14350)
                     /gene="rsgA"
                     /locus_tag="ACOIAS_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGZ2700569.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(14448..15185)
                     /locus_tag="ACOIAS_000851"
     CDS             complement(14448..15185)
                     /locus_tag="ACOIAS_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGZ2700570.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(15293..17308)
                     /gene="prkC"
                     /locus_tag="ACOIAS_000852"
     CDS             complement(15293..17308)
                     /gene="prkC"
                     /locus_tag="ACOIAS_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861606.1"
                     /note="PrkC, as found in Clostridium difficile, has a
                     kinase domain, two PASTA domains, and a C-terminal
                     Gly-rich repeat region; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing Ser/Thr kinase PrkC"
                     /protein_id="HGZ2700571.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(17325..18077)
                     /locus_tag="ACOIAS_000853"
     CDS             complement(17325..18077)
                     /locus_tag="ACOIAS_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HGZ2700572.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(18097..19128)
                     /gene="rlmN"
                     /locus_tag="ACOIAS_000854"
     CDS             complement(18097..19128)
                     /gene="rlmN"
                     /locus_tag="ACOIAS_000854"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HGZ2700573.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(19136..20461)
                     /gene="rsmB"
                     /locus_tag="ACOIAS_000855"
     CDS             complement(19136..20461)
                     /gene="rsmB"
                     /locus_tag="ACOIAS_000855"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGZ2700574.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(20523..21224)
                     /locus_tag="ACOIAS_000856"
     CDS             complement(20523..21224)
                     /locus_tag="ACOIAS_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HGZ2700575.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(21293..22045)
                     /locus_tag="ACOIAS_000857"
     CDS             complement(21293..22045)
                     /locus_tag="ACOIAS_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HGZ2700576.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(22055..22984)
                     /gene="fmt"
                     /locus_tag="ACOIAS_000858"
     CDS             complement(22055..22984)
                     /gene="fmt"
                     /locus_tag="ACOIAS_000858"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGZ2700577.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(22999..23439)
                     /gene="def"
                     /locus_tag="ACOIAS_000859"
     CDS             complement(22999..23439)
                     /gene="def"
                     /locus_tag="ACOIAS_000859"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGZ2700578.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(23457..25946)
                     /gene="priA"
                     /locus_tag="ACOIAS_000860"
     CDS             complement(23457..25946)
                     /gene="priA"
                     /locus_tag="ACOIAS_000860"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGZ2700579.1"
                     /translation="MKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVIKIGHRILVPFGK
                     GNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWMKNRYLCTYIECI
                     NLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVEKVIDSKGKIKVD
                     KLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKIN
                     VGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIY
                     KSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGL
                     DSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGAR
                     SALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNITLILGSATPSIE
                     EYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSLKLQKEIRYAIEE
                     NNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKE
                     CPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEA
                     DVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLITQVSGRAGRADKE
                     GKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLV
                     IKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQILFKDENIEINLLK
                     GIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(26221..27420)
                     /gene="coaBC"
                     /locus_tag="ACOIAS_000861"
     CDS             complement(26221..27420)
                     /gene="coaBC"
                     /locus_tag="ACOIAS_000861"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGZ2700580.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(27425..27691)
                     /gene="rpoZ"
                     /locus_tag="ACOIAS_000862"
     CDS             complement(27425..27691)
                     /gene="rpoZ"
                     /locus_tag="ACOIAS_000862"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGZ2700581.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(27694..28311)
                     /gene="gmk"
                     /locus_tag="ACOIAS_000863"
     CDS             complement(27694..28311)
                     /gene="gmk"
                     /locus_tag="ACOIAS_000863"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGZ2700582.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(28341..29222)
                     /locus_tag="ACOIAS_000864"
     CDS             complement(28341..29222)
                     /locus_tag="ACOIAS_000864"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGZ2700583.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(29226..30056)
                     /gene="dapF"
                     /locus_tag="ACOIAS_000865"
     CDS             complement(29226..30056)
                     /gene="dapF"
                     /locus_tag="ACOIAS_000865"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGZ2700584.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(30343..31377)
                     /gene="ltaE"
                     /locus_tag="ACOIAS_000866"
     CDS             complement(30343..31377)
                     /gene="ltaE"
                     /locus_tag="ACOIAS_000866"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGZ2700585.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            31673..33448
                     /locus_tag="ACOIAS_000867"
     CDS             31673..33448
                     /locus_tag="ACOIAS_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HGZ2700586.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(33663..35315)
                     /gene="cplR"
                     /locus_tag="ACOIAS_000868"
     CDS             complement(33663..35315)
                     /gene="cplR"
                     /locus_tag="ACOIAS_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Contributes to intrinsic pleuromutilin, lincosamide
                     and streptogramin A resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein CplR"
                     /protein_id="HGZ2700587.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(35614..36825)
                     /locus_tag="ACOIAS_000869"
     CDS             complement(35614..36825)
                     /locus_tag="ACOIAS_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGZ2700588.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(37109..37642)
                     /gene="pyrR"
                     /locus_tag="ACOIAS_000870"
     CDS             complement(37109..37642)
                     /gene="pyrR"
                     /locus_tag="ACOIAS_000870"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HGZ2700589.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(38002..38949)
                     /locus_tag="ACOIAS_000871"
     CDS             complement(38002..38949)
                     /locus_tag="ACOIAS_000871"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGZ2700590.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(38918..39364)
                     /gene="lspA"
                     /locus_tag="ACOIAS_000872"
     CDS             complement(38918..39364)
                     /gene="lspA"
                     /locus_tag="ACOIAS_000872"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGZ2700591.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(39674..40453)
                     /locus_tag="ACOIAS_000873"
     CDS             complement(39674..40453)
                     /locus_tag="ACOIAS_000873"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2700592.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(40508..40990)
                     /locus_tag="ACOIAS_000874"
     CDS             complement(40508..40990)
                     /locus_tag="ACOIAS_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HGZ2700593.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(41256..42677)
                     /locus_tag="ACOIAS_000875"
     CDS             complement(41256..42677)
                     /locus_tag="ACOIAS_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HGZ2700594.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(42823..43593)
                     /locus_tag="ACOIAS_000876"
     CDS             complement(42823..43593)
                     /locus_tag="ACOIAS_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2700595.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(43598..44800)
                     /locus_tag="ACOIAS_000877"
     CDS             complement(43598..44800)
                     /locus_tag="ACOIAS_000877"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2700596.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        15030 a         7847 c         4848 g        17622 t
ORIGIN      
        1 tgtaatataa aaatctatac tatagacttg attatggaaa gaaaattatc aaaatcatta
       61 caattagata tactatctct aatatcattg tcatgaatta tctttaaaag gatatcataa
      121 atggagttta tttttatata atcatcttta tttatagcca acatcatgat taatttaaca
      181 gtagaatctt cactccaata tattggttca tccaatatgg aaatacaaat cttagttctt
      241 atagagctta attccatagg atgaggtata gctaaaaaat catctatatt ggtagaagat
      301 aaactttctc tgtaaaatac agattcagca aatgatggaa atacaatttc actttgagtt
      361 aatttgttgc acatcaactt tataagctca tctttatctt tgattttagg attaacaata
      421 aataaatttt tatcaaagaa attgtccaaa agattactat aaatatggga attattagtg
      481 attgacttag atatattctc tatatctttt tttaatagtt ttaaatccac taatacaata
      541 ggaatactat catgatttaa aggaattgtc gatattatta tatctacatc tgacaattca
      601 ctagctaaaa attgattaaa tgatagacat tgaagtatat ttattttatc atgaaaaact
      661 ttgtttaatt gaacctctaa taacctactt gaaccatacc ctgaaccaca tactaatact
      721 acgtttttac acttaatatt actttgaaaa aatctctcta ttccagcacc tatatgcagg
      781 gatacatatc caatttcatc ttctgttaaa gagtaaatac tatttttaaa aactttttct
      841 atagcattta aagttatttc aaatgctaaa ggataattac tctttattgt atttataagg
      901 ggattcacct tatttaaatt ataagattta gaattgagaa ttgatttaaa gtgcagtact
      961 aaatcatcaa ataatacact atcatttcta aggtcaaaag tatattgatt gttgattact
     1021 tctaaaattt cttcaacata ctctataatt ttagtatcta ccctattgga aacgttatcc
     1081 aaaagagaac ttttagttat aaaatgatta tatagataaa ctctgtctgc ctttgaaata
     1141 attatagtat atttactctc aatataatta aatatatttt caacagttgt attcgattga
     1201 aaatctgtta aaacaacatc taaaacatta tctaagcagt aaccatcaag tattctactt
     1261 acagtaatag ctaaatggat aataaagttt tttagattaa aatctaggaa attgatatta
     1321 aatttattaa tttcacttaa cactaccctt tgaagctcta ttaaatctac attattaaat
     1381 aattctattt ctctatctgt aattccaatt atatagtttt caaaattcct atttatcata
     1441 ttattagaaa tacatcttcg aatatctatt tccttacctt caactttaac tccataatat
     1501 ggcttagaaa ctaatattaa attatacttt gacaaaatag gctttattct ttttatgtca
     1561 tttagtattg taaatttact cacatacaag ctattagcta aatcttctag ttttatatat
     1621 gtattgttat agagaatata ttttaaaata tattgatttc tttctatagg agaatctggt
     1681 atttcacttg aatccataat atcatcagat attaatgcta gatactgttg gtataatgct
     1741 aaattaagaa tatctatata atatccttct ccttttttta ttttaataat agcaccattt
     1801 ttctctaaag ttccatttat agaagtaata tcatttctta tagttctctc actcactttt
     1861 aaatgattac ttaaagtttt acaagtaatt ttatcatcac tttcatttaa taatttaaac
     1921 aacgtgagca atctcttgtg cataaatatc cccccttata gaatttcttc taaattatac
     1981 tatttacact catataatac aattactcta ataaaattgt attataataa tttatttcca
     2041 cttctatatg gaaattagtt attatcataa tataaatttc catgtcgaag tggaaattag
     2101 ttatatttaa tttaattagg ataatgatta caatataaat ataagttaag acattatgaa
     2161 tggagtggaa aaatatatgg tactacaaga aaaatatata tttatagata aagacttaaa
     2221 aagcaaagaa gaagtattaa attttattgc agatagtgct tatatactag gtattacaac
     2281 taataaagag gctcttttaa aagaccttct aaaaagagaa aatgaatttc caacaggaat
     2341 aaaagatgga tttgcgatac ctcatacaaa aagtgtaaat gtaaaggaag cttccatact
     2401 ctatattaaa acaactaacg agttagagtg gaaaacttta gatgatagta aggttagata
     2461 tgtatttagt ttgttagccc cacataaaaa tgaaggaaat atacatttga aaactctatc
     2521 taaattagcc acatgtttaa tggaggatga ttttacaaat gaagttaaaa atgctagtga
     2581 tgcaagtcat ttagtaaaat atataactaa taaaatgaag gaggaataat tatgaaaata
     2641 gtagcaataa catcatgtat agcaggatta gcacacacac ctatggcagc aaaagcttta
     2701 gaacaagctg catcaaaaat gggtcatgaa atcaaagtag aacaacaagg tgctatggga
     2761 cgagtaaata ctataacaaa agaagaagca caagatgcgg actttatact tatagcatct
     2821 gaccaaacta tatctggaat ggaaagattt gatggtaaaa aggtgataaa agtcaaaata
     2881 ggtatagcgt taaaaaaacc agaagctgtc ttaacaaagt gtatagaagc tatttcttaa
     2941 taaaaaaatc tcactctaat atagaatgag aaaaatcaaa aattaaaaaa ttgtctaatg
     3001 taattataac atattatcaa aaaatataac aaacttatat aggaggatta aaaatgagta
     3061 aaaaaaataa atctctttta agtgaactta agggacactt aatgacaggt atctcatata
     3121 tgttgccact tgtaataggc tcatctttag ttgtagctat cccaaagctt acagctttag
     3181 caatgggtat aacaagttta gatgcttata aaaatgtatc tggtttttat catattcttt
     3241 acttaatgga acaagttggt tggactggca taggtctatt aaatacggta ttagcaggat
     3301 ttatagcatt ttcaataggt gagaaaccag ctatgggtgc aggttttatt ggtgggctca
     3361 tagcaagtaa tactaatgca ggattcttag gagcagtaat agcaggtttc tttgctgggt
     3421 atgtttgtaa gtttttaaag aaaaaaatta aaatatctgg gtcagcatca ggaatgatgc
     3481 cacttataat aatgccactt ataacagttg gattaactgg ttttttaatg agtgttgtat
     3541 tagcaggacc tttaggaaac ataaatactt cattaaatgc ttgggtagct gaaatgtgtc
     3601 aaaatggaac aaattcggtg gtattggctt taattttagg cgcaatgata ggattcgatt
     3661 taggtggacc agtaaataaa gcagcttgga tggcaggaaa tgcattattg ttagagggaa
     3721 tctatctacc agcgatattg gtaaactgcg caatagttat acctccacta ggatatggtt
     3781 tagctacatt cataagaaaa tcaaagtttt ccaatgcatt agctgaaaca ggaaaaggca
     3841 atatagttat gggtataatt ggaataacag aaggtgcaat tcctttcact ttaacaaatc
     3901 cattaaaatt agttcctgtt aatatgatag gatgcgcttt gggtgtagga ttatcagctt
     3961 tgttaggtgt acatgctata atgcctccag ttggtggatt atatggattt atatcaatag
     4021 gttctggatg ggcttactta gtaggtgcaa tatttggagc tttagtaata gcaatattat
     4081 caacaacttt agtagacttc aatgaagatg aaactacgga aaatacagaa tttgatgaaa
     4141 tagagttaga tataatgtaa attaaaaact taattttaga taattgagaa ttgagtgtac
     4201 tctcaattct caattaaatt ttgggaggat ttttatgaaa aagacaaaag tacatgtagt
     4261 tccacacagt cattgggata gagaatggta ttttacaaca tcaaggtcaa aaatatattt
     4321 aatgaaagat tttcaagata tattagacat attggaagaa aaagaagact ttaaatactt
     4381 tactatggat gcacaagctt ctctcttaga tgattattta aaatggagac ctcaggatga
     4441 atatagaata agagatttag ttcaaagaag aagattagtt attggacctt ggtatactca
     4501 aacagaccaa ttggttatat caggagaatc tataattaga aacctttact atggaataga
     4561 tagatgcaat gaatttggtg aacctatgag ggttggatat gtaccagatt cttttgggca
     4621 atcagctcaa atgccacaaa tatacagagg ttttggaata gatagttcat tattttggcg
     4681 tggagtttct gatgatatgg tagatacaac agagtttata tggaaaggtt ctgatggttc
     4741 aaaagtatta gcagttcaaa taccatttgg ttattactat ggaggtaata taccagaaga
     4801 tgattctgat ttaaaagaat atttaaaaga tataatcgga aaacttaagg ttaaagcttc
     4861 tactaataac gtatattttc caaatggatt tgaccaagca ccaataagaa aaaaccttcc
     4921 tgatatatta aaaaaagcta atgaaataga taatgaaaat gaatacgaaa taagtgatat
     4981 agaaaactat atagattcag taagaaatga aagaaatgaa tttacagagc ttaaaggtga
     5041 atttttaaat gctaaacata tgagaataca caaatccata ttctcctcaa ggtcagacct
     5101 taaaataatg aataacaaat tagaaaatta tattacaaat gtacttgaac cattattaac
     5161 attaagttat tcacttggaa atgaataccc tcacctagtt ataaaagata tatggaaact
     5221 tatgtttgaa aatgcagctc atgacagcat aggttcttgt aatagtgaca caacaaatga
     5281 ggatgtatac acaagataca aacaagccag agatttgagt ataaatttat tagatttgca
     5341 tatgagactt ataagtactc aaatcaaaaa taataatgaa gaaataacat taacagtatt
     5401 caatggattc ccagaagtca gaaatgaagt tgtagaattt gatacttata ttccaggtca
     5461 agattttgtg ataaagaata aaaatggaaa aattcttaat tatataataa aagaaaaaga
     5521 agatattaca aattatatat taactcaaac aataagatta aacccaagta aaaaaatata
     5581 tatacccaaa actgtatata gagcaaaaat tgcactttca gtaaaagata taccaccaat
     5641 gggatatact caactagttt ttgaattgga tactaaaaat aaatgtgaaa tagaaacaag
     5701 taattgcaag gaaatagaaa acaaatacta caaaataaca gccaataaaa atggttcatt
     5761 agaaatatta gataaagaat ctaataagac ttataaaaac caagctgtaa ttgaagacaa
     5821 tggagatgat ggagattcat acaactattc tcctccaaga aaagatattt atgtttcatc
     5881 tttagattct gtatcttcag tagaagtgct aaaatcaaac attcaagaag aaatgatact
     5941 taaatattca ttgaccatac caacttcact ggaagaaagg gctataggta aagtttctgt
     6001 gaaaatgcca gtaacaatga aaatcacctt agaacctaaa gaacaaataa ttaagtttaa
     6061 tgttaatata aaaaataacc aagcactatc tcatagagta agagttttat ttaatacaga
     6121 aatagcatct aagttttcta tagcagatca acaatttgga ataatacaaa gaccaacagt
     6181 attagaaaaa gaccttgcat tgtggaaaag agacaattac tcatggcaag aaaaacctat
     6241 aactatcgaa ccaatgcaaa gttttgttac tttagaggat ggacaaagag gaatagcatt
     6301 aataactggt tgtgtaagag aatatcaaat agttggtgat aatttagata caatagcatt
     6361 aacactattc aggtcatttg gatatatggg aagagaaaat ttactttata gaccaggaag
     6421 agcttctgga gaaaaaataa tagcaacacc agatgcacaa ttacttaaag agttaaactt
     6481 tgactttggt ctttacatat ataacaataa atttgatgaa gcaaatgtag ctaatacagc
     6541 taagagattc ttaacaccta tacaaatata tgaatatgct gattttttaa atggtagact
     6601 tatatttgca ttcaatgacg aaaaacaaat atatgaaaat gaatttagtt tgatgaatgt
     6661 aaataattca aatttcacag taagtgcaat aaagaaagaa gaaaaaggag atggaattgt
     6721 agttagaatc ttcaatggta tgaaaaatga ggatgctaag ggaagtctta acatcaataa
     6781 aaatgtcaat gatgcttata gtgcaatgct cgacgaaatc tatgataata ctagcaagct
     6841 taaagtaaat actaaaactg tagaggtaaa ttctttatca cattgtaaag tacaaacaat
     6901 agtaattaaa taatataaaa tagcttatac aagatttcaa aataaattgc tagtactatg
     6961 ttaatagtaa agccagatat tatgctattt ataagctctg taacactaaa taacattttg
     7021 taaaataaaa aggtgaacct tgtatattag gattcacctt ttttaaataa atcaacattc
     7081 tgttttaaaa taacatttat aagctgtttt gaaataatgc ttacaaatta ttttaaaaca
     7141 atacctataa atttttattt aaagaataca cataaatatt tccagattct tttattctat
     7201 agactttata attattaaag aagtctaatc taatttttgt taaaagtaat tgtaactttg
     7261 aataaatcac catccacact tatattcatc ttaccaccct gtaattctat aaaacttttt
     7321 gctatcgcta aaccaagacc agaaccttct gtgttacgtg atttatctcc tcttacaaat
     7381 ctctccatta tatcatctac actaaaatca atctcagatg cagacatatt tttaagcata
     7441 atatttactt tattatctgt ttctaaaata tcaatataca ctcttgaatt tggcattgca
     7501 tatttgctta tatttattat aagattttca aatactctaa atgtacgctg actatctaat
     7561 ggtaatatta ttttttcatt agaaaagtta ttctttacta ttagagatga atctgcaagt
     7621 ttgtcatcta attctaaaag tgtctgcttc attagagata caatatccac atttactata
     7681 ttcaaataaa catctccact atttgccttg cttacttcaa acaaatcttc tattagatgt
     7741 tgcaatcttt gtgactttct gtctaatgta tcaatataca acttacgatt ttcatctgtt
     7801 atactttcat cttttaataa atctatataa gtgattatag atgttagagg tgtttttaag
     7861 tcatgtgata catttgatat aagctctgtt ttcatctttt gactcttaac ctcttcgtca
     7921 acagcttttt taaatccagt ttgaatattt cctaattcat cttttatagg attaaaaaat
     7981 cctaaatctt tttccatatt tacatctaag tttccatctg aaattttctt tgtaacatct
     8041 aaaagttgat tatattcata tattgtcttt ttagagtaat ctgacagtat cttaaataat
     8101 actactgaat atattaatgc gagtattatt ccaaagaacc acatactaca cattataact
     8161 ataatcaata aatttattcc caaaattgta actatagttt tatttggctt ttctttcaga
     8221 tttattgttc taatttgatt aataactgct tttcctgctt tttttaacca tacaaaaaac
     8281 attcctgtca cagtatgttt tgtaaaatac tctcttattc caacatttaa aatgtgtttt
     8341 aaaagtatta taaatacaaa tattattgag aatattacaa accaataagt taaattcatt
     8401 aaacttacca atatactagc cattttagca cttatgtcat tgtttataag gtactctaca
     8461 taattcccac tcaaggtatt acttattata acagtaggat ataaatctac aagcccctgt
     8521 ataatagcaa gagcaataaa gagtatctct aacggtattc ttgataatgt atcaaatcct
     8581 agcaactctt tagattttct atatgggaat gctaaagcta caataacaac tatagctaat
     8641 ataattttta aaatcattcc agaaatctta taataagaat tattttctac attgcttata
     8701 tatgaaatta taccatcatc aaaataagta tttccataac catactctac actatcacct
     8761 ttacttatta ctttaggtac agcatatact aatgttatat tttttattgg agataactta
     8821 tatgaaattg aatttcctcc taaacttgta tccattaaat cttctcttgc tttagataac
     8881 aacatttgat taataatttt ttgatttact ccataagaat ccacaatatt tacttttcca
     8941 tctttatcaa aattcattac aacgtagaat ctatagtttt cttttatatc tgtaatctgc
     9001 ttttgtcttt cctcatcatt catttctgta ttttttgact gattttcatt agaattttct
     9061 tttgaagcat cattttttgt tgtatcaagt actcctaatt ctttattatc attaaaaaat
     9121 tcaaaattgc tttctttatc aactaaatag tactttaaat tctttcctga cgctttaatg
     9181 aaatgctccc aattttcaaa gttttcatta aaatctgcat taatactatc atctgtataa
     9241 taagtttctg aaccatcata agtatctttt tctttactta cattttttat atagttcata
     9301 gtttcttcat tgataaatag ctttgatggt tgtatcatct taccttcttg ttcttccttc
     9361 atattatgat ataaaacata acttccttta tatatactat ctacaaagct ttcctgttca
     9421 aatggattct ctgctccacc tttagcactt gcttgtatag tagaatattg actaatcata
     9481 cctaatgatg ctgataacat aagtataaaa actataatag taactatata tttatcttta
     9541 aattttttca attttgtatc caacacccca caccaccttt aaatatttag gattttttgg
     9601 gtcaatttct atcttttccc ttatattcct cacatgtacc ataactgtat ctgtatttac
     9661 agcattctca ttccaaactc tttcatatat ttcatcagca gaaaaaactc ttcctgcatt
     9721 tctcataagt aaacttagta ttttaaattc tataggagtt gtttttatat actttccatc
     9781 aatactaact tcttttgtat tttcatttaa ttctaatccg cctattgcaa acacactatc
     9841 gtcactttct accttttgct ctttatttcc aaccatgttt agatacttag tgtaccttct
     9901 aagttgtgaa ttaactctag ccaatagttc taatggctta aaaggttttg ttacataatc
     9961 gtctgctcct atattaagcc ccattatttt gtcaacttct tctgattttg cagatagcat
    10021 tattactgga aaattatagt tttctctaag cttcattgta agtgttatac catccatttt
    10081 aggcatcatt atatcaacta tagctaaatg tatttcttgg ttttctataa ccttaagacc
    10141 ttctaatcca ttgtatccct taaaaacatt atatccttga tttaatagat atatttctat
    10201 agcatctgct atctcctttt catcttctac caccaaaata ttatatgaat tcattctaat
    10261 ctcctttata ttaatttata aattttcttt acatatatga ttatacaata tgatagaagt
    10321 ttttccattt atatatatat ttatatcttt tgttctggtt atttgtattt aatgtccatc
    10381 ttagaaacct ccttctagtt taaatttaat attttttcat tttgttttat atacttaata
    10441 ttaaaattaa aaacttaaga tattacatat acttttctaa agattttcta aagatttaaa
    10501 tatatttaaa attaatatac aaaaaaaagt agttcttatt gtattaatta aattaatgat
    10561 ttgaaatata aaattaatat caataaaacc tactttttaa ttttttaaat atttattttt
    10621 aatttatttt gtttttaatt tacttcgatt ttaaaatgtc cttgactttt attttataaa
    10681 tgtacatatt tcttctactg gttttttaga tggactcttc aaattgtcct ctggcactcc
    10741 aagagctaac attgcaccta aaaaatactc tcccttttca aaaccagttt ctgcttctaa
    10801 aacagcttct atctcatctg ctgcatagtt ttgacttgtc aaccaacaag aaccatatcc
    10861 aagttcaatt gctgatagtg taaagttttc aattgcagca cctagactct gcatacctgg
    10921 atttcttata aataatttat ctatagtctc cttaggtgca tctataagct ctaattcata
    10981 atatcctgat ggattatata cctttgtaaa aactaatact aaaactggag cttttaagta
    11041 aaatagtgta aaatttttaa caaattttct aaatctatta gccttatcca ctgatacttt
    11101 atccatttct acaagtattt cttgttgctt tttagttatc acatctgcta tcttttccat
    11161 taaatcacgg cgttttataa caacaaaatg ccagttttga atattcttgc ctgatggtgc
    11221 tgcacctgct gcttttatca tttttaaaat atcttcatct gatacatctt gatttttaaa
    11281 ctttcttaca ctttgtcttt taaaaatagt atcttgtaat tccattttta aatcccccca
    11341 taactttttt attattttaa tataattttt aaactcatct tattttaata tatcatgttc
    11401 taacaaatct ttaaatgaat ctataaaata atcatataca tctacattcc caaacccata
    11461 tttggcatat ataaaaggta tacctacaaa cttagcacta tttgcatcac cttgcgtatc
    11521 accaacatag attggattta ttaatttatt tctttcaatt ataagtttat tattttctcc
    11581 tttatgcaaa ccagtattac caggacattc aaaatctata aaatactgtt ctagtttatg
    11641 tgcttttaaa aaagattcta tatatccagc ttgacaatta cttactataa ataatttata
    11701 ttttttagat agtaatttaa gagtatcttc tatatcatca aataatttag ctccattttc
    11761 agataaatag tcacattctt tcaaagaaca ttcattcaat aaattcattc tttcttcttc
    11821 acttaaattt ggaaataaac gatttgctat ttcctcaaaa gaaagcccca taagacttct
    11881 aaataaatct tttgtgacac ttaattctat ttccttatgc tcacttaaaa catcctgcca
    11941 gactttgcat acttcttcag ttgaatccca caatgttcca tctaaatcaa aaataatact
    12001 atctaccaca aaaaatcccc cttatgtcaa agtttattat atatttataa ttatatacta
    12061 aaaagcagat acaatcataa atatatttta cttgattgta tctgctctac aatataaaac
    12121 caattgtcaa aatcaaattt tataaatttc taactacaag tacacagcct tgctctactt
    12181 taatcttaca agaattactc aacataacat ttgatattcc aataggtaca gagtatttca
    12241 tataataatt atcgagaggg tattctaagc cagtaagtgt tacaccctta gcatcaccat
    12301 ttatagctat tacagaaata gtgtcccctg ggtttccata caatgaaatt tcctcatttt
    12361 caactatata catctcttct ttatcagaaa gtatcctaga atatatacca tcttctttta
    12421 aataatataa tagctttata tttgcaagag tgtggtcaat ccttccccct aaagcaccaa
    12481 aaaaatcaat atgatttgct ttaagtgtct tagctaaaaa aagacaaagc tctgtatcag
    12541 tctcatcttt tttagatgga aatttttcaa atttaacacc tttattttta tagaagttta
    12601 tcttttcttc ttctactgaa tctaaatctc ctaatatata atctggcatt atttccattt
    12661 tataagtgtg atttgcccct ccatctgcac atattatgca gtcataacat tccttaatta
    12721 taatatctct tgtaacttcg taatcttcaa tttcgccatt taaaacaata cagattttca
    12781 ttattaaatt taaactaatt cttcaagaga agcattcttt ctaaataaat taactgtttc
    12841 ttgaatgtca tcacttccaa atattgcaga acctgctact attacattag ctcctgcttg
    12901 tacaacttcg gctacgtttt tagggcttat accaccatca acttctatat caatgtttaa
    12961 tccttgacta tctataatag cttttaactc tttaatctta tctaaaacac aaggaataaa
    13021 tgattgtcct ccaaatccag ggttaacaga cattaataat accatatcta aatcagatag
    13081 tacatgttta atcgtatcta ctggtgtagc tggattaagt actacaccag cttttattcc
    13141 atgtgatttt atattttgta tagttctatg tagatgaaca caagcctcct ggtgaacagt
    13201 tattatatca catcctgctt ttacaaattc ttcaatatac tggtctggat tttctatcat
    13261 aagatgagca tcaaatacca tatttatatt ttctttcttt aaacttttga ctacaagtgg
    13321 tcctaatgta atgtttggta caaagtgtcc atccattaca tcgatatgaa gatattcaca
    13381 tcctgcactt tctacttttc ttacatcttc taataatttt gcaaaatctg ctgataatat
    13441 tgatggtgct aatttaatca tgtttctaat acctcctact attattttgt cttatttcat
    13501 gtagcagctg tatataacta tcatatcttt ctttagatat ttctccattt gtaacagctt
    13561 cttttatagc acaattaggt tcattttcat gaatacactt tgaaccaaac ttacaatcat
    13621 taaatttatc aaattctata aaataatctt taagttcaac ttcctctata tcttcaagtg
    13681 caagggaact aaaacctggt gtatctgcaa ccattccacc aaattctaat tttaaaagtt
    13741 cagcgtgacg agtagtatgt tttccccttt ttattttatc acttacaact cctgtttgaa
    13801 gtttaaaatt ttcatctatt tcatttaata agcttgattt tccaactcca gatggtcctg
    13861 caaacactac tacattttct tttaattctt cttttacttt atctatattt aacttagtaa
    13921 tattgcttac aggaatgact ttataaccac taagttcata tatattttta acagtttcta
    13981 atgtatcatt atcatctaaa tctgcttttg taaggattat tacagtttct aaattttctt
    14041 tttctgccaa aactataaat ctatctaaaa gagataagtt tggtttagga ttttttattg
    14101 cgaatactat taatgcttta tctacatttg caataggagg tcttataagt tctgtatctc
    14161 tactgtcgat ttcttctaag ataccctttt tttcatcttc atcaacaatg cttattttga
    14221 ctctatcacc aaccaaaggt gttattttct gctttctaaa tatccctcta gccttacatt
    14281 cataaatacc attataagta tctacataat aaaatccact aatccccttt ataatttttc
    14341 cttctagcat caaatgcctc cctttataaa atttatataa acacatactg tttatgagta
    14401 gtatatcgtg ttattctttc ctatttttat ataataccac atttttttta aataacaaat
    14461 cctccgtttg gactaattac ctgaccagtg ataaaatttg atttgtctga tgccaagaaa
    14521 atagcacaat ttgctatatc ttctggagtt cctagcctca ttagaggggt ttcttccaca
    14581 agagcgtcta tatcttcctc attatatcca gataacatgt cagtattaat gacacctgga
    14641 gctatgctat ttactctaat atttgatggt cctacttctt tagctaatgc ctttgtcata
    14701 cctattatac ctgcttttgt tatagaataa tgcacttcac aagacgcacc tgatataccc
    14761 cagattgaag atatattaat tatatttcct tttttttctg atatcatgta ttttaaagca
    14821 acttgtgaac aataaaagct tccttttaaa ttaatattca tcatattatc ccaatcttca
    14881 tcagttatat ctgtaaacaa cttatcttga cttacaccag cattgtttac aagtacatcc
    14941 aatcctccaa attcttttat acaataatct accatatcct ctacatcttc tctatttgaa
    15001 atattagcct taaaaagttt tacagaaaaa tttttttcat ttaatatagt gtacaactct
    15061 ttagcttcat tttctgactt attaaaattt actaatacat tgtaaccttc ttttgcaaaa
    15121 gctttagaca tagctttacc aatccctcta gctcctcctg taattaaaac tgttttcttc
    15181 ttcataaata cttttctcct tatattataa ccaattcagt attttataca aaacaaactg
    15241 ccctttaaag aaaatatttt ctataaaagg acagctctta ttttattaat atttattctg
    15301 ttttaccaat gtttgcgcca gttcctgatg aagttcctcc attatctcct gagtttgagc
    15361 ctcctgaact atctccattg tctcctgagt ttgaacctcc tgaacttcct ccattgtctc
    15421 ctgagtttga gcctcctgaa cttcctccat tatctcctga gtttgagcct cctgagtttc
    15481 caccactatt tcctgagttt gaacctcctg agttacttcc attattgcct gagtttccac
    15541 cactatttcc tgaatttgag cctcctgaat ttccaccact gtttcctgag tttgaaccag
    15601 gttctgttgg tgtattattc tctggtgttg ttggtttttt atctggtact gttggagtct
    15661 gtgtatttga tttttctgaa cctttgctta catatagatt tacctcatca ccttcctgag
    15721 ctcttgatga gccagcacct ggactctgac ttaaaactgt accttcttta tatattgagc
    15781 tgtattcata cttaacagtt cccactttta agttattttc ttcaataagt tgttttgcat
    15841 tactcaaagt aagtcccact acattaggaa cagatgcctg acttgaacct ttacttacga
    15901 caacatttat agtatcaccc tttttgacat ttgtacttcc accttcaggt gtctgtgata
    15961 ttatacaatt ttcatcaact tcactattat actcttcact ttttacactc aggtataatc
    16021 cttctttttc aacagcactt tgagcttctt ctaatgtcat atttacaaga ttaggaacag
    16081 tcaaagattt attgccaaac cctccagcaa ataaaaactt atatgctaag aatacttgag
    16141 cacacaatat cagtatcaaa acagccgcaa ttacttttaa tcttcttcta gattttgggc
    16201 tttcctgttt tttcttcttt ttcttagttc tcttacttgg agtactctta ggtctctgat
    16261 tctttttagc tctcattatc tcttcctctt cttcatcttc atcatcatct tcttcataaa
    16321 aatcatcata gtaatcttca tcatcatcca aatcagctac ttcaacaggc ttaaccactt
    16381 tttcaggagc tggttttgca agtgttggat ttacaacttt attaatttct ttttcatcaa
    16441 ttttcttagt tgcaaaatca tcatattctt ttataaaatc taaatcaata tttttttcta
    16501 tatactctat atcttctatc aattcttcag cagtttgata tctatctgca ctagattttt
    16561 ctgtaagctt ttttattgta gttcttacac tttgaggaat cctaactttt tcttctgatg
    16621 taaagtctat atcatcatta atgtgttgaa gtgctataga aattggacta tctcccctaa
    16681 atggaacttt tcctatcaac atttcatata aaactatacc tagagaatac aggtcagcat
    16741 tattagttac aaatttacct ttagcttgct ctggtgaaaa ataatgcact gaacctatta
    16801 tactaccaat attagtcatt gttgaatttg aaacagcttt ggctatacca aaatcagcca
    16861 cttttactac tcttccttca tttgatatga gaatattatg aggttttata tctctatgta
    16921 taatcccctt tttgtgcgct gcactaagtg ccatagctat ttgttttgtt atatctaatg
    16981 cagtatattc atctaatata ccttcatctt gtataatctc ttttagattc ttcccatcta
    17041 caaactccat tacaatgtag tgaacttttc catcttcacc aacatcataa acatttacta
    17101 tatttggatg tgaaagactt gctactgctt ctgcctctct tttaaacttt gttagaaatt
    17161 catcatcatc tacaaattca ggtctaagta ctttaagtgc aacagtcctg tttaataatc
    17221 tgtctttagc ctcatataca aatgccattc ctccatcacc aatcttcctg atgatttcat
    17281 atcgatttcc taaaattgta tctcccacaa tatcaccgtc ctaattatac ttttatcact
    17341 ataaccgaaa cattatcttt accagaaaca tcattggcca tacttatcaa ttcttcactt
    17401 atattactag ttctctcata ttcataatct aatattaaat ctcttatatc ttcatcatca
    17461 ataaacccag ttaatccatc tgtcgcaaga agtattatat cacttttttt gatttctttc
    17521 ttaaaaatat ctacaacaac catatcatct gttccaattg ctctagttat tctattcctc
    17581 tgtggatgtc ttcttgcctc ttcttctgta atgacatggg ccatcataag ttcttcaacc
    17641 acggaatggt ctatagttat tttatcaaat ttttcatctg ttaacaaata acatctgcta
    17701 tctccaacat ttgccacata taagacatta ttatacacta tagccgtaac taatgtagtt
    17761 cccattcctt caaattctat atcttccata gattttttgt gcacaattga gtttacatta
    17821 ttataagcct gttttaatat atcatctata tagtctattt ttacattatc atgttgtaat
    17881 aagttttcct ttagaaaatc aattatattt tctactgcta atttactggc gacttcacct
    17941 tttttatgtc cacccattcc atcagctatg gcaaatattc ctattggacc atgttctgta
    18001 tctataactt ctccctcaca atagtcttca ttgttttttc ttatttttcc tatatgggag
    18061 gcacaactat aaaccatacg acttcctcct tacaaattac ttgtattttc ttctcaattg
    18121 cccacaagct ccactaatat cagaacccat agacatccta actgtggcag gtatattgtt
    18181 cttctccaaa ctatccctaa atttataaat aaatgcttta tctggctttt catattctct
    18241 ttcttcaact ttatttattg gaattaaatt tacatgacat agcatacctt ttaggagctt
    18301 agctaatgcc tttgcttctt tttctgagtc atttacccct tttataagag aatattcaaa
    18361 tgtaaccctt ctatttgtct tttttatata atatctacat gcattcaata tttcttcaat
    18421 agaataagca tttgctacag gcataatttt tcttcgttct tcatcatatg gtgaatgaag
    18481 tgataaagct aaatttatag ctatttctaa gtctgccaac tcatacattt taggtactat
    18541 tccacaagtt gaaagagtta tatgcctata ccctatatta agaccatttt tttcgttaat
    18601 tatttttaaa aattgttttg tattttcaaa gttatcaagt ggctcaccac tccccatcaa
    18661 tacaagattt gataccctct taccagtatc ttcttgaatt ttaataactt ggtctaaaat
    18721 ttcccatggc tctaagtttc taattaatcc atccattgta gatgcacaaa aattacatcc
    18781 catcctacaa cctacttggt tagaaacaca gacagtaact ctactatcat aatccatcat
    18841 cacagtttct attatatttc catcatctaa taaaaacaaa tatttttttg tattatctac
    18901 cttagattct aactttaatt ctatatctat atgccctata catgaaacct cttctaattt
    18961 atttctcaaa ctttttggta tattgttcat atcatcaaaa gtttttgcac ccttatatat
    19021 ccaagagaat atttgacttc ctctaaaggg cttttcatct atagttttca taaattcttt
    19081 aagttcatct tcagtaaaat tttttaatac tatctttttc ttttccattc tctcactacc
    19141 ttattctctt taattttgct atgaaaaaac catctatacc atgaatatta ggatatatct
    19201 ttaaatatcc tttatcttga ttgtctaagt ctacatttac tgtatcaatt ggaacaaatt
    19261 caaagttgtg attttcttca acaaaagaag taactatatt tatattctcc acatcttgaa
    19321 ttgtacatgt actatatact aaagttccac caactttgac atatttagat gcattttcta
    19381 aaattttctt ttgtatagaa gtgatgtctt ttaactcttc ttctttctta tacttaattt
    19441 ctggtttacg tcttattata ccaaaaccag agcaaggaac atctgctaaa acataatcaa
    19501 atttacttat actattttca tcaatctcag aagcatcaaa tctttctaca aacacatttt
    19561 taagccccaa tctatttaca gtagagttta taagctttag tttatgctca aaaatatctc
    19621 tagctactac ttgaccagta ttgttcatta aagtagctaa gtgagtagat ttccctccag
    19681 gagcactaca tacatccaaa acaaatgagc cttcttttgg attaattact ttccctacta
    19741 tcatagaact tatatcttga attgtaaata aaccagcctt aaataattca tttttttcta
    19801 tatttttcat tttttcgact ttaatagctt cttctaccat aggaactttt aaacatacaa
    19861 tacccatttc acttaatttt tcaataagtt cttctctact tattttaagt gtattcgttc
    19921 ttatatagat actaggcttt tcattatttg cttccaacaa atcttcagca aattcttgtc
    19981 caaatttatc tatccagttt tttataatcc aagagttata ggaatacttt atagataagt
    20041 attttacact atcttcctca gttatgtcca ttatagtctc tttagctctt atttcatttc
    20101 taagaatagc atttatgaaa ccagaagatt ttttatcata tttcttagat aattttacag
    20161 tttcattaac tgctgcataa tctgatatac tatctaaaaa taaaatctga tatgttccca
    20221 ttcttaaaaa gattttaaca taagttgaca ttttttttac ctttattttt gaaagtttat
    20281 tgattatata atctaaataa tatttatttt ctattactcc ataaattaac tctgttgcaa
    20341 gtcctctatc catatcactt atttctaaat ttttaaaatg cttatttatt gcaatatttg
    20401 aataattgtc atttttttct atatcacata atactttaaa tccaacttct cttgcatcca
    20461 tataaatttc tcccttttat taaacataaa aaagggttgt ttctaaacaa cccactttac
    20521 ttttagtcat ttcttctttg agctattagt aacaatctaa gtaattgaag tattgaaacc
    20581 aatgctgctg caacatatgt taaagctgct gctgaaagta ctcttctact ttgtcttgac
    20641 tcactttcat ctataatacc taaattccca agttgaacga ttgctctact tgaagcatta
    20701 aattctacag gcaatgttat tatttggaat aacactgaag ccgaaaacaa tagtatacca
    20761 atttttaaaa atggaccagc cataataaat cctaaaaata ttaaaaacca agatatactt
    20821 gaagcaaaat tcactactgg tactaaacta cttcttattt gaagaggtgc ataaccttta
    20881 gcatgttgta tggcatgacc acattcatgt gcagctactg caacagaagt tatagatgtt
    20941 ccataatata catcttctga caaccttaca gcctttcttc ttgggtcata atggtcactt
    21001 agatgccctc taaccatttc tatctttaca tcatacaaac catttgaatc aagtactctt
    21061 cttgctgttt gctctccagt atatcctcga cgtgtattta ctcttaaata cttatttgtt
    21121 gttgaactca ctttaaattg tgcatatata gtaagtaata tagctggaat taatacaacc
    21181 attgttgggt caaatcccca aaaaccgcca tatacagggt acataaataa cactctccta
    21241 aaatatattt ttattataca tcctcttaat attcacttaa cttaaaaaca gtttaattac
    21301 aaactaatat attttttcct gttaaaaagc ctatagcttt ttcaatatcc atcatgtcta
    21361 cattagtatt tacacaaggt ccattaggtc ttttgttaaa tacacctaaa acaggaattt
    21421 tcttaatatc ttgtattcca gatgtcaaat ctctttcaca tgcaacagct acaacagctt
    21481 taggtttagt gtccattata atttttctag ctagagttcc tcctgtagct acaaatattt
    21541 ttacattagt tttttccttt agcttaatta attctcctat gttgcatctt ccacattctt
    21601 tacaattatc tatatcatta gtaactttta atttacaact attctcttga atacaatgag
    21661 gtattaaaat tattatatcc tctggattaa aattatattt attactgtat atatattcat
    21721 tatttaactt tatatatatt cttctaatat cactttttga cataccaaat gatgatgcaa
    21781 tgaaagatat aataggaaat aatccactga caatattaaa atttattttc attatatttg
    21841 agttaacata ctttccttta attactctat aagtaactac tgttgaacat agtattataa
    21901 gtattagtga tattattata atatttatca tcaaggagaa tatttgagtt aaacttatta
    21961 taaaaaaatt aacaactcct atcaaaattc ctaatattaa aatcaaaatc ccaacacttg
    22021 ttaaatactt ctttatatct gtcatacttt tcacctaacc caatactaaa ccagtttcta
    22081 tagtattccc ttttatatat tcaccaacca acattctctt tttattcggc atctgaattt
    22141 cttcaattaa aagtacatta tctatagtac ttacttttat cccatctttg cttacatcaa
    22201 ttattgtacc tacatctttt gtgctttttt catctaacac tttagttttc caaactttca
    22261 ttatgacatc attataggta gtataagcac ttggccatgg atttactcct ctaactaaat
    22321 tgtgtatttc tctggcactt tttgaaaaat ctatgttacc taatgattta ttcattattg
    22381 gcgcatagga aaattcttca tgattttgta cttctcttgg agcatttcct tcttcaatta
    22441 atcttaatgt ttcttttaaa gtttctgctc ctatattcat cattttatca tgaagttctc
    22501 ctgctgttat attttcatca agatttactt ctgtctttag tatcatatct ccagtatcta
    22561 atccttcatc catatacata gttgtaacac cagtcttttc ttcaccattt attattaccc
    22621 aatttatagg tgccgcacct ctatattttg gaagtaaaga aacatgaaca tttatacatc
    22681 caagtttagg aatctctaat atttcttttg gaagtatctg tccaaaagct acaactacta
    22741 ttacatctgg atttaaagat ttaattttat ctataaattc tttatctcta gcctttactg
    22801 gttgatatac aggtatatta ttttcaattg ccagttcttt tactggactc atacctagtt
    22861 ttttacctct tccttttggt ttatctggtt gagttacaac acccaatatt tcatacttct
    22921 catctattat tttttgtaaa catggaactg ctatatcagg agttcccata aatactattt
    22981 tcatcaaatc aactcctact acttttctat tttgtcaaca aataaaattc catctaagtg
    23041 gtctatctca tgacaaaatg ctctagctaa aagttcttct ccctctaact ctattgtttc
    23101 accatttcta tttaaagctc tgacctttac ataatttggt cttctaacat ttcccgcttc
    23161 tccaacaaca cttaaacatc cttcctcatc tatttgctcc ccagatgttt ctattatttc
    23221 aggatttata agttctataa gctcttctcc aatatctatt actactactc tttttaatat
    23281 tccaacttga ggtgcagcca gtccaacacc atctgcatca tacatagttt ctgccatatc
    23341 atctaataat tgtattatct tttcatcaat tttttcaacc ttttttgatt tttttcttaa
    23401 tactggttct cctatttgaa ctatttgtct taaagccatt ataattcctc ctaaatttat
    23461 aatacactgt ttggattaat atctatagat acattgatat tcttatcaaa tatcaaatct
    23521 cgttttgtta tacatatata ttttattata ccctttaaga gattaatttc aatattttca
    23581 tctttaaaga gtatttgcca tctataattt tgatttattt ttgatattga acatggattt
    23641 ggacctaata taaatcctaa atcatttata cccctttttt ctaataaata aatcaatgat
    23701 gcatgcatat tttttatatt ttttatcact aatctttcat cttcaccact aacaactaca
    23761 cttaacatat tattaaaagg agaatatcca aatgcttttc ttatttttat ctcatcttca
    23821 taaaaaccct cataatcata ttcaatagca tgcttaatag catagtgttc agtgtcataa
    23881 gtctgaagta ctacttttcc ctctttatca gctctccctg ctcttccaga gacttgagtt
    23941 atcaattgaa atgtagtttc aaaactttta aagtctggaa aatttaatat catgtctgca
    24001 gacaaaatac ctactaaagt tacattctca aaatccagcc ctttacttaa catctgtgtt
    24061 ccaataagta catctgcttc tttatcttta aatttattta gtatctcatc taaagcacct
    24121 ttttttgaag ttgtatcttt atccattctc aaagttttta tatctggaaa aatatatttt
    24181 aattcctctt caattttttg agttccaact ccaaatggct taacataagt acttttacat
    24241 tcaggacact cttttggaat ttctttctca tatccacaat agtggcatct tccagtattg
    24301 cttttcttat gataagttaa agatatatca caattttcac attgaaacac atatccacac
    24361 tttctacaag atacaaaatt tgcataacct cttctgttta aaaatagtat tacttgatta
    24421 ttttcttcaa tcgcataacg tatttctttt tgtaatttca aactaaatat acttctattt
    24481 cctttatcta actcatcttt catgtctaca acttcaatgt ttggaagagg cttatcattt
    24541 gctctacttt ttatatttat aagtttgtat tctccagatt ttgccctata atactcttca
    24601 attgagggcg ttgctgaacc taaaataaga gttatattat tttttaatgc cataaatcta
    24661 gcaacttcta tagcattaaa ttttggattc ttctcagact tatatgatga ctcatgacat
    24721 tcatcaatta ttattaagcc taaggaatta aaaggtgcaa ataaagctga cctagctcca
    24781 attaacactc ttactttacc tgctttgaca gctttgtaaa cgtcatgttt ttctccttca
    24841 gatagtttac tgtggaacac tccaactaaa tctccaaacc tactctttag cctatctatt
    24901 gtttgaggag ttaatgctat ctcaggtact aagaatatac tgtctaagcc ttgatttaga
    24961 gcaaattcta ttatttccat gtaaacttca gtttttccac ttccagttac tccatgaatt
    25021 aagtatggtt ttttatcatc tacaaacata ttcgacttta tctcatcaac tgcttcttgt
    25081 tgctcattgt ttaattttat actttttagt acggatttgt aaatactttt tggctctcta
    25141 taataatcct taaattctag ggttattagc tttttcttac ttagagaatt tatactttgt
    25201 tttgaagcat ttaataaatc caataaatca tttatctcta cattttcatt atttttaaga
    25261 aaacttaata tctctttttg tttacttccc acatttattt tattttgctc tatataatcg
    25321 tcaatcttat cactttctaa actcaaagat atataacata ctttcttttc atttttgtga
    25381 tttttatatt cccaacataa ttttataatt ccatttttat tcattgtgta caaagaacta
    25441 tttaagtttg gtatatattt taacttatca accttaattt tacctttact atcaatcact
    25501 ttctctacta tttctctatt tttatcactt aaagttgata ttttttcctt caattcaata
    25561 tcatttaatc cacttaaact ttcacctaat aggactactt tataattatt taatttgtat
    25621 ccctttggat atatcaaatt tatacattct atataagtac acaaatatct atttttcatc
    25681 caataaacca actctaaatc ttcttttcta aatatcggat tttcatctaa tatatcaata
    25741 atttctttta tttttatttt ttcatctaag ctatctgtaa attgaaatac aaaggcttct
    25801 gttggtttat taccttttcc aaatggtact aatattctgt gcccaatttt tattacatca
    25861 gataaaaata caggaatttg atatgtaaat aaattatctg tatatatagt attacttcta
    25921 actattacct ttgcatattt tttcattata aaacctctac ttaaatttaa tatattgttc
    25981 atattttaat atactttaca agatattaaa atctcagaat ttataagcaa tttcaagtta
    26041 ttaatattaa ctttatttta ataattaact attaattata ttttatactt tatgacaaaa
    26101 taaatgctga aaactacaat ttaactgaca tttgcgagtt tttactcgct aaaattaaat
    26161 tgcagctttc agccacttta gctgtgataa aatacacaaa tatcatattt aaaaaaggtc
    26221 ttatttctca agcaacgctt taactttatc taaaataaca ttggctactt cttctttttt
    26281 catcttagga tactcagtaa tatttccctc tacatctatt atttttacaa tattagtatc
    26341 agctctaaaa ccagcacctt ctttagttaa atcatttgcc acaataaaat ctaaattttt
    26401 cttttttatt tttcctttgg catgttctat aaggtcatta gtttcagctg caaatccaat
    26461 tagtatctta tcttctttta tttttcctat ttcataagca atatcataat ttctacttag
    26521 ttctattgat aaatcatcat tcttcttttt aatttttttg tcagaataag tttttggttt
    26581 ataatctgca actgctgcac tttgaattac tattttattt tcacttaaat ttgatactac
    26641 tgcttcatac aaatcttgtg ctgactctat ttgaacaaat tttttaagat tttgtggaat
    26701 agaaagattt gtaggacctg atattaatgt tacatcagca cctctagcta tagcttctct
    26761 tgctatagaa tatcccattt taccagaaga cctattagtt aaatatctta ctgggtctat
    26821 actctctaca gttggaccag ctgtaactat catactttga ccttttaaat cttgctcttt
    26881 agtaagatta gtcaaaactt cctctactat tatatctgga ttagctagtt tacctgttcc
    26941 aatatctcca catgcaagcc ttccactttc cggttcaata aatttatatc caaagctctt
    27001 taaagtttgt atattttttt gaacaattgg attttcatac atattagtat tcatagctgg
    27061 tgctataaga acctgtgctt ttgttgccat aacagtagtt gtcaacatat cgtcagctat
    27121 accatttgca atcttgccta taacatttgc acttgctggc actattaaga ataaatctgc
    27181 tttttttgct aatgatatat gttcaacttc ccaagttttt ggttcttcaa acatatcttc
    27241 tactacataa ttttgactca gcgattgaaa tgtaagtggt ttaacaaatt ctgttgctga
    27301 ttttgtcatt ataacatgga tatttgcatt tagttttttt aacttactta caacatcaca
    27361 agccttatat gcagctatac cgccacttat gccaattaca acagttttat cttttagcat
    27421 gaccttactc ctcttctgaa atttgttggt gttgctcagc atgatgtcta gcttcttcta
    27481 tctctatttc ctcttctgtc aataatctat atgttatttt tccacttgca acttctttag
    27541 ttgctacaca tacaggcttt tccttagttt tatttgaaac atatggttct tctccatcaa
    27601 ttaattttct tgctctcttt gaaacagtcc ccactaaata gtatctgttg tctatttttt
    27661 ctagtacctc attgatagat ggttttaaca taattataat tcctccttaa atttctctat
    27721 tataatattt ttatatctgc taactttatt cttttcagat gatattatac cttctatttc
    27781 tttagcagat gtttttacat cttcattaaa tataaaataa tcataatcct taatcatttt
    27841 aatttcttca tatgcacagc taaatctctt ctctatttca gcttctgttt ctgtacctct
    27901 tccaacaatt ctatttttta attcttcaag tgatggtggt agtacaaata taaatactcc
    27961 ctctgggcaa acttctttta tttgttttgc tccttgcatt tcaatctcca gtaatacatc
    28021 ttgtcctttt tctaaacatt ccattattgc cgcttttgga gttccataaa aattatcata
    28081 aatttgagca tactccaaaa actcaccttt ttctatcatc tcttcaaact tttccttact
    28141 tatgaagaaa taatttactc catcaacttc tccagttctg ggttttcttg tagtagctga
    28201 tacagaaagt tttattgtat cattttcttt aagtagttct ttgcaaatag taccttttcc
    28261 tgcacctgat ggtcctgata caactagcaa taatcctttt ttcacaaaca tttaagcatt
    28321 ccccttctaa tattgtattc ttactctata ttttgtattt gttctcttat tttttccaat
    28381 tcgcttttta cttctactac taaattagtt atatttatat cagatgactt cgaacctata
    28441 gtgttagttt ctctattcat ctcttgtatt aagaaatcta tttttctacc tatagagtcg
    28501 tctttaaata tagtattttt aagttgccct atatgacttt taaatctaac tatttcttct
    28561 gttatactgc ttttatctgc atatattgca acttcttgtg ctaatctatt ctcatctatt
    28621 atacttgggt catccagtaa ttcacttatt ctcaaattca atttttctct ataatcttct
    28681 acaacactac ttgaatattt ttcaatctct tctatatgat tttttaataa atcacatctc
    28741 atggcaatat cttcagctaa ttttttacct tcttcacttc tcatttctct taatttgatt
    28801 aatgcatctt caactgcttg attcaacatt gaccacaata aatcttcatc ttcttccttt
    28861 tcttctattt tcacaatatc aggaaacttt gcaacattca taacgctaat atcatctata
    28921 atatcaaatt tattttttat ttcttttagt atgtctatat actgagatgc taatccctca
    28981 tcaaaattta agtttacatc ttctttgcct aataagtcaa actttatgta taaatctact
    29041 cttcctcttt ttacataatt ttttactaaa tttctaactt tatcctctaa aaatgaaatt
    29101 tttcttggaa gacgtatgtt tatatctgaa tacttatgat ttatcgtttt acactctact
    29161 aaaaagtaat aattatcatc cttgtattct cctcttccaa aaccagtcat acttatagcc
    29221 attaattaaa cctccaaaat accttcacat atttttactg ctggacctgt catataagag
    29281 aaatctccta catctatttt aactgttcca ccttgagacg ttacatttac actactttct
    29341 accatattaa gtaaattgca cactattgct gatgcagtca taccagtacc acaacctaaa
    29401 gtatatccac aacctctttc ccaagtataa acatgtatat tatacttatc ttctaccttt
    29461 acaaaattta cattcgtttt ttcaggaaat attttattat tttcaatctc ttttccatat
    29521 ttacaaacat catctacatc taattcatct actataacta ctgtatgagg aacccccatc
    29581 aacatagaag taactttaag ctccctatct aaaaccttta tactttcttg cacaaaacgt
    29641 tctttatttg taagtacagg aacgtcctta gctataaaac ttccttttcc catattgact
    29701 cttatagagt ttatttctct attagtaaga tttactttta tatcttttat accatctaat
    29761 gtatctactg taaaactttc ttttttaact atacaattat cataaacaaa tttacaaaaa
    29821 catctaaggc catttccaca catagctgct cttgaaccat cagaattata atataccatt
    29881 tctacatctg caacttttga atctttcaca actaacaatc catccgctcc tacagaaaat
    29941 cttctatggc atactttttt tgctaaagca gtataatcag atggatttat ctggtcgaat
    30001 cttccatcta ttgctataaa atcatttcct attccatgta gtttccaaaa tttcataagt
    30061 atcgctcctt cgtaaattaa cgcttaaact attataacat aaaaaaattc ccataaactt
    30121 ataatttatg ggaaaagttt tgtaatgaca actataattt gaggatgtga gggagagttg
    30181 ccattacaat tagggtaatt atattatgtt tttaagctag ttctttctat gtttatatat
    30241 taatatttct atatgaattt tatgtatgtt ttatttaaac tgtctatgaa ttttttaatt
    30301 aaacatctgg taactagcta taatccttct agtagatatc tattaacata cacctattaa
    30361 ttctttaagt gtttttaaaa catagtctaa atcttcctta gtaatatcta catgagttac
    30421 aaatctatat tcaccatttt ccattttatt aatttttata tttctttcaa aaaattcttt
    30481 tattaaaaca tcctctttta taatatcttc actcatctta aaaaatacca tacttatatc
    30541 tggattaaca ttgtttactt caatgccatc aattttattt aattcacaag ctaaatattt
    30601 tgcattttca tggtcttcat tcaatcttaa agtcattttt tcaatggcaa ttataccagc
    30661 tgctgcaact atacctactt gtctcattcc cccacccata actttttgct ttcttctagc
    30721 ttttgctata aattctttat ttccagcaag tatagaaccc attggtgcag ctaaaccttt
    30781 tgacacacag aacataacac tatcacaaca ctttgctatt tctttagctt ctacacccaa
    30841 agttaatgct gcgttaaata atcttgctcc atctaaatgt actggtaagt tatgtttctc
    30901 agctattttt ttaatttctc ttaaattatc aagactcaca actgttccac ctgaatatgc
    30961 attttctaca catataagcc ctgtctgtgg ttcatgtatg tcatctcttc taattgcctt
    31021 ttcaacaagt tcaggattta gagctccact ttcgtcttca tctatagttc taagctgaac
    31081 tccagctatt acagcaggtg ccccaacctc atgagtcaca atgtgataac ctttaccaat
    31141 aattatttct tgccctctta ggcaatgagt aaatagagct aattgattcc caaaagttcc
    31201 acttggaagg aaaatagcat cttcttttcc aactttagct gctgctaatt cttcaagttt
    31261 atttacagta gggtcatctc taaagattga atctccaaca ggagcctttg ccatagcttc
    31321 tctcatttcg tcagtaggca tagtaacagt atcacttctt aaatcgataa atctcatttt
    31381 ttcaaccttc tttcatattt atatactata gaaccttaaa attgattttt tctgtaaagt
    31441 tcatatactt tcatagctta tactctacac aataattata ctatatttac tttatattta
    31501 atctacttta aattattaat tttaaaaata aagtttcctc tctaataaat ataaataatt
    31561 atacaattta aatattaatc tctattgttt aaattccccc tagtcacaaa gcgtatatta
    31621 tgtgtataat taatttactg actcattttg gtcaaagaaa ggggtgctat aaatggcatt
    31681 agatggatta gttatacatt caatagtcga tgaattatca tcaaaactta caggtggaaa
    31741 aatagataag atacatcaac ctgaagatga tgaggtaatt tttaatataa gaaataataa
    31801 agaaaatttt agattggttt taagtgcaag tgcctcaaat cctagagttt atcttacaag
    31861 taattatcaa aaagaaaacc ctctaaaagc gcctatgttt tgtatgttac ttagaaaata
    31921 catacaaggt ggaaatatag tagaaatttc tcaaataggt tttgaaagaa taataaaaat
    31981 aagtgttgaa tctttagatg aattaaaaga aaagacagtt aaaaatatta tgattgaaat
    32041 tatgggaaga catagcaaca taataataac tcatggtgaa gaaaataaaa ttatagattc
    32101 tataaaaaga gtccctttta gcataagtag agttcgtcaa gttttacctg gtcatgatta
    32161 ttcattgcca ccagaacaaa acaaactcaa ccctcttgat gatatatcta aggatttgtt
    32221 tattaaaaac ctagaggaat tggaaggacc tatctttaag tctatatatt ctaaatttct
    32281 aggtataagc cctattatag ctaaagaaat ttgctacaga gcaggtgtaa atcaaaatgc
    32341 tatcataaag gatatatctg atgaacaatt tgatgcttta cacaaagtat tttgtaattt
    32401 attcaatgat ataaattcta acaaatactc tccttgcatt attatagata aaaaggtgga
    32461 taaggtagtt gatttcagtt gtataaacct tacattattt agcgatttat catatataaa
    32521 taaggatagt atgtctcgta ttctggaaga tttctataga actaaggata ttaaagatag
    32581 aattaatcaa cgttcatctg atttaaagaa gagtatatct gttaagcttg atagacttta
    32641 taataaactt aaaaaacaag aagaagaatt atcagaatcc gaaaatgcag acatttataa
    32701 gataaaagga gaattgatta cttcatatat ttatatggta gaaaaaggta tggaaagtat
    32761 tgaagttgct aacttctatg atgaaaattg taatgatgta acaatagaat taaataaaaa
    32821 cttgacacca tctgaaaatg ctcaaaaata ctttaaaaag tataacaaaa tgaaacatgc
    32881 taaagtcgag atatctcatc aaatttcctt aaataaggaa gaaatagatt acttagaaaa
    32941 cataatttta agtattgaaa actgtgaaaa tttagctgaa ttacaagata taaaagaaga
    33001 acttgctaaa gttggataca tcaagactca aaagaaaaac agtaaaaagg atactatccc
    33061 atctacaaaa cctcatgaat ttgtgtcttc tgatggattt aaaatattgg ttggtaaaaa
    33121 caataagcaa aatgactatc ttacactaag acttgctgat aatgatgact tatggatgca
    33181 tacaaaaaat attcctggtt ctcatgtaat aataaagtgt gctggtaagg aagtccctga
    33241 taatactgta tttgaaggag ctatgctagc agcattcttt agtaagtcaa aactttcttc
    33301 tcaggtgcct gtagactata ctaagagaaa aaatgtgaaa aaacctagtg gttctaaacc
    33361 aggtatggtt atttatgaaa ctaacagtac tatttatgtt actcctgaag aagaaacagt
    33421 agccaagctt aaggttaaat ctgaataaat tctgcactaa atacaaaata aatgttagac
    33481 aactacacat tatcattcta aataaaaata gtggccttct tataaagtac aataataagg
    33541 ccactttcta attagcttac ttttaatttt ctaacaaaat acttttctgt tttaataact
    33601 atacttatat tcccaacaaa tattttcagt cttcttatat agatgtaatt aaaaagttaa
    33661 cactaaaagc tattttttaa tttattgatt tgttttagta attcattata ttctgattca
    33721 tacaattctt tttttagatt atctttttct atagataaca tagatataac agttgtaagt
    33781 ctattttcta atactagaag attttctttt ttgatttggt tttctctact aggagttttt
    33841 ttatttctgc taattgtata ttcgtcataa ttacaatcaa actcccttat tttagcatct
    33901 tttatctcta taatatagtt acaaatatgt gatacaaata ctctatcatg agacactata
    33961 agcatagttt tatcagtatt tatcagtgcc ttttccagtg cttccataga ttttatatct
    34021 aagtaatttg ttggttcatc aaatactaaa aaattattat cttccaatat tattttacat
    34081 agtgctattt ttaccttttc tccaccacta agtactttta cttttttgta gacatcatca
    34141 cctttaaacc caaataggct taagtttatc cttataaaag attcatcaaa tgaactatta
    34201 actttagtat tgtatagaac actcttttcc tcatctaata aactttgatt ttggtcaaaa
    34261 taacctactt ttaccttgtt atttattttt atattgtcat ttttatcagc caatatttct
    34321 tttatcagag tactttttcc acacccatta tcacctaaca atgctatctt tttacctctt
    34381 tttatcttaa aggaaacatt atctaacagg agcttatttt ctaactttag agtcatatct
    34441 tttacctcta ctagattttt gctaattatc tccataccat cttgcatctt aatgttcatt
    34501 tctttagaaa ctttaggttt attctttacc tcaagtttat caattctgct ttttatagcc
    34561 ttcacatttg agtccaactt tttcttatta gtctgaccac ccattttgtg caacctagct
    34621 tctgaattac ccattctttt tggtgttttt cttatgccat cacttaaagc ttttttatat
    34681 aaaagagctt tttcaagcct ttttttctct ttaacatatt cttgatactc aaaatcagct
    34741 ctttttactt cttcatcttt ttgcattaaa taatcagtgt aattgccttt gtaagctttt
    34801 agttttccat cttctagttc taatatagtt gtacataact catccagaaa acgtctatca
    34861 tgagatatta ataataatgc tccctcatgc cttttaaaca tatcttccaa aaccccaatg
    34921 ctttttttat ctaagtttga agttggctca tcagctatta tgatattttt cttattactc
    34981 agcgcatctg ctatttttaa cttaaccttt tcccctcctg ataagtgttc ttcaaattta
    35041 tctggagcat ttaaaagact cttctcctta ctatgactac atgtttcaat attattttca
    35101 ctctgactta tataagaata gctttctgta aggtaagtat atccttcatc aatttctatt
    35161 tctcccaata ttgcttttaa taatgttgtt tttccttgac catttgaacc aactagacct
    35221 attttgtcat tttctaatat ttcaagttta tctatatcca aaattaattt atctgcataa
    35281 tatttcttta aattctctac ttttactaac aacataaaaa atccctcctt aacacactta
    35341 aatataagga gagattctat aaataggcat aacaaataaa cgtatagact ataatctact
    35401 ctccttaatt taagcattgc aaataaagcc accaaaatag gcttttcaaa aattacaata
    35461 ctttaaatta taaaaaatag attatattct cattgtccta gttaccttgc cctttctttc
    35521 atttattttt aatcattata tcaacattaa ttacaaatgt ctatagaaaa ttaactaatt
    35581 ttagttattc aatatacaac ttattcttta aatttattct tcttcaactt taaagtcctg
    35641 attatttaaa attctgttta atacaattcc aacaattgca gctaaactaa gaccagttat
    35701 ctttactgta cttgttatag ggattccaac agaaatacct ttattagata aatatgttgt
    35761 tccaagacct attaataata taactgctat gattaatata tttattttac tttctacaca
    35821 agaactgtcc cttatagttt ttaatcctac ataagttatc atcgaaaata gcattatact
    35881 aattccaccc ataactgaac ctggtatact ttgaagaaat ccaccaaact tgcctacaca
    35941 tgcaagcaat atagcaaata ttgctgtacg tctaagtatt gaagggtcat agtttttagt
    36001 tatagcaagt actgctgtat tttctccata agttgtattg gctggtcctc ctaaacaccc
    36061 tgcaactata gtcgcaaatc catccccaag taatgttcta tttaatccgg gattttctat
    36121 aaagttttta cctactacag caccattagt agtcatatct cctatatgtt ccataaatac
    36181 agctaataca actggagata taatcactat tgctcctaaa ctgaacttag gaactgtaaa
    36241 gttaggtatt gcaaataaac ttgcttctgt aatagtagtt atatctacta atcttaatat
    36301 caaacagatt atatacccag aaaaaactgc tattaatatt cctaattgtt ttataaatcc
    36361 tctaccaaac ttattggtta aaattgctat tgctaaagtt ataaatgcta tcacaaaatt
    36421 ggctgaagcc atactaaaag ctgttggcaa taaatttaat cctataacta ctatcatagc
    36481 tcctgtaact tgtgcaggaa aatataattt aattttattt acaccaacta cttttactat
    36541 aatcgacatt ataatataaa ttaatcctgc tactattata ccaccttgag catatgctaa
    36601 atctccacca tatgcctctt tagctgctag tataactggt ataaacgcaa aacttgaacc
    36661 taaaaaggca ggtacttttc cttctgtaca aaaatgaaat atcaatgtac caacaccagc
    36721 acagaaaatt gcaactgacg gattaaaacc tgtcaaaatg ggaactaata ctgtcgctcc
    36781 aaacatggct aatagatgtt gtaaagacat tatcgttttt ttcataaaaa cctccaaaat
    36841 tattatatat aaataaatcc tctgtttaat taatatcttt taattaatta tcattcccta
    36901 gattctattt tttaacctct atatacttat tatataattt atcattattt tacattttac
    36961 gacatcaaaa actcaaataa aggagaataa aaactacatt aagactcaca tccataaaat
    37021 tataatattt attttgtgca atctatgtaa gctttatcct cctccctgac tattcaatgc
    37081 taagctttta tataatactt aaaaataatt attctttgat tgtaactgaa tcttctccat
    37141 caatttctaa taaactgaca gatataattt catgtctaga agtaggtaca ttttttccta
    37201 cataatctgc tcttataggc aactctctat gccctctgtc cactagcact gctaattgta
    37261 ttgatttagg tcttcctata tctataatag catctagtga agctctcaca gttcttccag
    37321 tgtataaaac atcatcaact aaaactactg tcttatcatt tacattaaaa tctaaataag
    37381 ttcctttaac tacaggttca acatgaattt tttccaaatc atctctatac aaagttatat
    37441 caatatctcc tgtatctact tttgttcctt ctatttgttc tattttctta gaaattctat
    37501 ttgcaattgg cacacctcta gtctttattc caactaaaac tagattttct acacctttat
    37561 ttctttcaat tatttcatga cttattctag taattgctct tgctattgcc ttttcatcca
    37621 ttaattgagc tttttctacc atatcataat tattgtcaaa gatataatat atctatttga
    37681 caaatctact cctttctttt aattttttat aaatttattt ttatttaaaa tttcaattta
    37741 aacaacaaaa aaacttctca tcacaagaca agaagctctt atataaaaaa tatacagtgc
    37801 aatttagcaa ttgtcttacg atatctctgt atcgtttaaa gataattttt taatctatca
    37861 aatcaaatta tatatttata tttcaaattt ttcaagcttt tttaaacaaa atactttaat
    37921 ataagatatt ttatttaaat ttcactaaat ttatttactt atataaaaac aaaaaatgat
    37981 ttataagtat tatacaaata gttattttat ctttttaata acttccttaa aatactcagg
    38041 taattctgaa ttaaattcga tatattcctt agtagtagga tgtataaatc ccaacttctt
    38101 agcatgcaaa gtttgtcctt ttaatttaaa tttagtttct tttggaccat atacataatc
    38161 acctaaaagt gggtgattta tagacaaagc atgaactctt atttgatgag ttctaccagt
    38221 ttcaagccta gctctcatat gtgtaaactt atcaaatctt tctacaactt caaagtgagt
    38281 gattgcattt tttccatctt ttactacagc catttttaat ctatccttag gatttctccc
    38341 tataggttta tcaacagtta ttttatcttc tttaaccaca ccatagcata taaattcata
    38401 ctccctagtt atagtatgtt cctttaactg ttctgctaga aaattatgtg cataattgtt
    38461 cttagctatc ataagaagac cagaagtatc tttgtctatt ctatgaacta taccaggtct
    38521 aataacgcca tttattgatg acaacttatc tttacaatga tataaaatcc cgtttactaa
    38581 cgtattttca tagtttccag gagcaggatg gacaaccata tcctgaggtt tatttattat
    38641 taagacatca tcatcttcat atacaatatc tattggaata tcttgaggta aaacttctaa
    38701 tactttaggt tctggtatct ctataacaat attatcttcc tctttaacca gatatctagc
    38761 tttttctact ttattattaa cctttacttt tccatctttt ataatttttt gtatataact
    38821 tcttgacata tcacctaatt gttctgctag ataaatatca agtctaactt cttcttcctc
    38881 ttcaagtact aaaaactctt ttacttcatc cattatatca cctactttta ctttcaaaaa
    38941 ataaaacata tatacataaa aacacagttc ctacaactac aaatacatca gctatattga
    39001 atacatattc ccatataatt ctaaagtcga agtaatctac tacaaaacct aatcgtactc
    39061 tatcaattag atttcccaat gcaccagata taattaatat aattcccaac cttccaaata
    39121 tatgtacttt ccttgtatta agatagtata gtccaaatac tgttgcaagt aatgcaacaa
    39181 ttataaatat ccattgatta ttttgtaata atccaaatgc tgcacctcta ttttcgacat
    39241 aagttaaatg aaatacatta tttattattg gtattgttga tacatccacc aaattattca
    39301 atacccatat tttagacagt tggtctaaac ctatgagtag aattattatt aatatatata
    39361 gcaatgattt ttccccctat aaattattta aaaaccaatt ttatttgaag cacactatta
    39421 ttattataca aaaaaaatgc tctaaagagc aactttatta aaattgtatt ttcttaataa
    39481 agctagaaca aaatttaaaa tagtactact ttttttatat tgtagatttc tacatattta
    39541 aaatcatatc attatgtata aaaaatttaa aaataccata ctaatcaata ttttttgtgt
    39601 attttataga ttaaagatta atgatgtctg taatttaaac ttacaatcac cactaatctt
    39661 caaacaaata tttttacttt tgcacttgct tatttttatc taaatgttgt ataagttcag
    39721 atactgttac gaatttatat cctctctctt ttaatttagg aattactact tctaaagctt
    39781 gtatagtttg tgatttatca tttcttttag tgttataatc atgtaaaagt attatattac
    39841 cattttggac ctttgtaagg atagtattta taattgcatc tactccaggg ttagaccaat
    39901 ctctagggtc taagtttgac caaagaatta tattcatgcc tttagaaact ataatatcac
    39961 agacactttt actcatggct ctatatggag gtctaaatat ctttggttct actcctatta
    40021 cactttttat aatattttgc gtatctgtta tttctttttc aacactacta taactatttt
    40081 ttgcaacatt tatatgtgta aatgtatgat tccctatttc atgcccctca tcatattgtc
    40141 tttttaataa ttctttgtta tattctactt tttctccaac tacaaaaaaa gttgctttta
    40201 catcattttt ctttaatata tcaagtactt gtggtgtaaa atcctcatct ggaccatcat
    40261 caaaagttaa tgctaaaatt ttttcatcct catttccttt ttttattatt acatcttcat
    40321 aatcgtcttt tatattgtca tttaaactat atgcttcttt tgaactatct tgattatgtg
    40381 tattatgatt atatgtatat acacctacta tagataataa acatatacat ataccccata
    40441 ttattttctt catgtcatta cctcttttct aattcattct agcaatatat tttataatgt
    40501 cagtttttta atctctcaat attccatttt tatttaaatc agttaaattt gttaggtctt
    40561 ctgaataaaa gttagaatct tctacatcag ggttttgcat tgcatcacct aataaattat
    40621 tttctttctt tgcacaatca ttacataaac ttgtatctgg caatatatcc aatctttcta
    40681 aatctatttg attatgacat aaatcacaaa tcccatatgt tccattctct attttagata
    40741 atgctaagtc tatttgatac aatcttccct cttcatgatt tattagactg ttttgcatat
    40801 tttgcatata taaatctgtt cccatgtctc ctatatgatt atcataacta gataattctc
    40861 ctgatgaata cttttcactt gtatgtttag ttgtatctcc aaataaagta ttatctttca
    40921 tatcctctac taaattagtt aaattttctc tttctttcaa taatttatct ttatattttt
    40981 tagtatccat ttttaacacc tctaatttta atatttacat cataaaaata gtaagtggaa
    41041 aagtaaatta aacatacaat aaaaataaga tttgattcga aaaatggaat gtctcaaaat
    41101 gaaattttta gttcatttaa ccctcttttt tatatgaaat caaatatatt tttatccttt
    41161 cagcaataaa aaagagactt atctctattt tgagatagtc tcttctttga acataattta
    41221 aatacaattt tttaattttt atagttatta ttttactaac ttgccaattt atgattattt
    41281 tgttctttaa tatgttttgc ttttttaaag aataaaagac caagaattat tgctacaata
    41341 acacctattc cattagatat attaactggt agtctaaatc cttcttttgc ttgcattata
    41401 taactaaatg ttactacagt cataaaaagt gctggtaatg tagttatata atggttttta
    41461 tttgctttga ctaaatatgc agaaccagtc cacaacatta tcattgcaag tgtttgattt
    41521 gcccaagaga aatatctcca aattatatta aaatctataa atgtaagagc aacaccaact
    41581 gcgaataatg gtaaagctat taaaaatcta tttttgactg catcttgatt ataattcatt
    41641 gcatctgcta ttgtcagcct agcacttcta aatgctgtat ctccagaagt tataggacat
    41701 gccacaactc caagtatagc taaagctcct ccaataggcc ccattaatcc aacagatact
    41761 gtatttacta ctgttgctgc tccaccatca ttaaatatca cattaagttg aggtatgcca
    41821 tggaaatatg acatggctgc tgctgcccaa actaaagcta ttataccttc tgcaaccatt
    41881 gacccataaa atactggtct tgcctcactc tctttctcaa cacatctcgc cataagtggt
    41941 gattgagttg catgaaaacc tgatattgct ccacatgcta ttgttataaa taagtatggg
    42001 aataaaggtg ttccatttgg attcatattt tgtaaattta tatttggtat gttatacccc
    42061 tttattatta atcctccagc tacacctact gccataatta ataatgctgc tccaaataaa
    42121 ggatatattt taccaatcaa tttatctatt ggtaaaacag tagccataat ataataaagt
    42181 attatcactc caacccatat ttgtgcgttt acacctgtca atgtctttaa taatcctgct
    42241 ggacctgtta caaatacaac tccaaccaat actaaaagaa taacactaaa tattctcatt
    42301 gctatttttg caccatttcc tagataaagg cctaccaact ctgatgcaga tgtacctttg
    42361 tttttcattg atagatatcc tgaagcaaaa tcgtgtactc caccagcaaa gattgttcca
    42421 aacacaatcc ataaaaatgc tgatggtcca aataatgctc cttgtatagc tccaaatatc
    42481 ggacctaacc ctgctatatt tagaaactga attaaaaaga ttctcttcca tttcattggc
    42541 acataatcta ctccatccct acaagctata gctggagttt gatttttgtc attggttcca
    42601 aaaacctttt ctacaaaagt accatagaca aaataaccta ctataagtac tactattgaa
    42661 cctaaaaagc ttaccataaa ttcatcccct tagtttttat ataattgctt tataaatttt
    42721 tatgagttta agaaaactat ttccagttca aatagtaata cttttttatg ctaatataaa
    42781 tattaattac attaagtgca tatttaagta catgaaatga ctttatatat gcattaacag
    42841 cctaaattct tttactttgc ttctacttac agttacttca taatcactat tttttaattt
    42901 taatatgtat gtattattaa accatggtat aatttctttt actttggtta agtttacgat
    42961 ataagaccta tgtgttctaa aaaatgtttt atcatttata agattttcga cttctgatat
    43021 gccttctttt aatataaact tttctttctc agtatatata aatgtctgcc tttcattagc
    43081 ttcacagtaa tatatatcat caatatctat tacaactaat ttatcacctt tccaaaggct
    43141 aattttatgt gttatttctt ctatctcctg atttactgct tccttcttat atttgtataa
    43201 attactattt acattaaata gttcaatatc actcatctca cttttttcta atttattcag
    43261 catagaaata attctctctt cagaatatgg tttcaatata taatcaaata catccaaaga
    43321 aaaagcatct actgcataac tttcatatgc tgttataaaa acaattttag gtcttgattt
    43381 aaactgattt aatgtttttg caagtagcat tccatcaaga tgaggtatat ttatatctaa
    43441 aaaaattaca tctattttat tttcctgaat aaagtctaac acctctatac cattggtaaa
    43501 ttcacttact acctcgattc cacttttatt ttcaataaaa tatctcaatt cctttcttgc
    43561 tggaaattca tcttccacta ttatagctct catacttcta ctcctttaca taaaatacta
    43621 tcaaagtccc attatctatt cttcttatat taagccctct tccatacatc agtttaagcc
    43681 ttaaatgtac attataaagc ccaatcttat tttctggcat attatcttga tatacattat
    43741 ttataatttc ctcacttata cctataccat tatcctctat tgatattctc acagtatttt
    43801 ctaatgaata cggtaacttt tttatggata ttttcactac accagctcta ccactttcta
    43861 aaataccatg tattattgca ttttccacta atggttgtat aatcaaactt ggaatcttta
    43921 tatttacatc ttctatatca taagatacag ttagtaattc tccaaaccta gctttttcta
    43981 tctctacaaa agatttaact tgttcaatct ctttatttat atcaattaag ttatcactat
    44041 attcaagatt atatcttaga taagttgata aatttattat taactctcta gccttatctg
    44101 gactaaatct tataaatgaa gctattgtgt ttagggaatt aaacaaaaaa tgtggattaa
    44161 tttgtctttg aagtgctttt atttctgctt tatttctcgc ttctatatct tttttatctt
    44221 tctcaatccc ctctactata gatataacaa atcctattcc aatctgccca actattaaag
    44281 gtaaatatat tccaccgatt atttgaattg ctaaagaatg tggatatgat attaagtata
    44341 taagtaatat agtaaaactt tcagaaatca tgcctaccaa tattccatac attactctat
    44401 gttgttttgg tatcctctta tatagaaatc ctgataatac acctgctagt atacttgata
    44461 aaagacatgg aacagatgtt atccccccaa tatccataaa atatctatga atccctgaaa
    44521 aaactcctgc tgtaatacct accatcggac caaataaaat gccacctgat actatagata
    44581 ttattctagt gtttactatt gaccccatat aatttatacc actataagtt ccaaatatgg
    44641 ctaaaaaagt aaaaactaat gctatacaga ccaagtcatt aaaactatat tcttcctttt
    44701 gaaatatctg cttaaatatt tttaactttg ttattagaag aaacaatact acaataaggc
    44761 ttgtcttttc tattaatgcg attaaaattg atgttttcat aatctccctt taaaaaataa
    44821 atttttacac actctaaatt tattatggtt gttataaatt acatatttga agtaatccta
    44881 tcacaaaact ttccacattt aatcttttaa ttaaataaga aatagttgta cttctattaa
    44941 caactaatac tagctagcat aagcttgtaa taacttgtag ttattattaa ctttacaatt
    45001 ctagtattta attttatact ttagaaatct cttttcttag aaataatact ttcgaatatc
    45061 atataataca atattatgta gattcttact catacaacat atttctttaa aaattttata
    45121 ctattctttg ttcaaatatc tcatttgatt taattgtgtt aaacacacat tatcatctct
    45181 ctgttatata tttattatat agccgtatat cgataaagta aataaatccc atatttaaaa
    45241 taatatgttt gacaataaat tattataatg tatatttacc ataataattt gtcaaccttg
    45301 aaagttactt ctataattat tgttaaataa ttcttctaaa aaaaata
//
LOCUS       DBGJFU010000016        43522 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.016, whole genome shotgun sequence.
ACCESSION   DBGJFU010000016 DBGJFU010000000
VERSION     DBGJFU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.016"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..394
                     /locus_tag="ACOIAS_000878"
     CDS             <1..394
                     /locus_tag="ACOIAS_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HGZ2700597.1"
                     /translation="HIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNSKLSRLI
                     SDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPS
                     CNETHDRDINASINILKEGLRLITIQNK"
     gene            515..1762
                     /locus_tag="ACOIAS_000879"
     CDS             515..1762
                     /locus_tag="ACOIAS_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGZ2700598.1"
                     /translation="MSNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIII
                     METGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMI
                     ARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLITFAAVLSALYWSIMPWLLRK
                     YVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGG
                     MAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAF
                     QGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILIYNYFNVFNLLLIP
                     LVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIF
                     GGNDLSLFGIISRFIGSLFGGIL"
     gene            1765..2505
                     /locus_tag="ACOIAS_000880"
     CDS             1765..2505
                     /locus_tag="ACOIAS_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2700599.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            2828..4138
                     /locus_tag="ACOIAS_000881"
     CDS             2828..4138
                     /locus_tag="ACOIAS_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HGZ2700600.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            4236..5153
                     /locus_tag="ACOIAS_000882"
     CDS             4236..5153
                     /locus_tag="ACOIAS_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGZ2700601.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            5169..5978
                     /locus_tag="ACOIAS_000883"
     CDS             5169..5978
                     /locus_tag="ACOIAS_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGZ2700602.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDTMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            6036..7784
                     /locus_tag="ACOIAS_000884"
     CDS             6036..7784
                     /locus_tag="ACOIAS_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HGZ2700603.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            8088..9068
                     /locus_tag="ACOIAS_000885"
     CDS             8088..9068
                     /locus_tag="ACOIAS_000885"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HGZ2700604.1"
                     /translation="MFFLSISFIGFYLVIRDNSDTVKMQRGINIGNALESPKDFPWDV
                     KMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKIDKYVDYALDKDLIV
                     VLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELLNEPKENLYSQLLNE
                     YIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVVSFHYYEPNDFAFQG
                     NIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIFLGEFGITKEAPEAS
                     RRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILSALIEKR"
     gene            9073..11379
                     /locus_tag="ACOIAS_000886"
     CDS             9073..11379
                     /locus_tag="ACOIAS_000886"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2700605.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            11390..12466
                     /locus_tag="ACOIAS_000887"
     CDS             11390..12466
                     /locus_tag="ACOIAS_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700606.1"
                     /translation="MKTLKVFFCSTKYLILVFIFFINTTFISSATSDKILDLSFKKIE
                     TDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNFYIEIQNKNKSSQR
                     INLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLIN
                     EESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKLRFLNNIKIIGDEE
                     VQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTLSNKAKPGQIILQV
                     NVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMKKINFMNNIITFVR
                     ILFVSLVIICFGIYLYWKKCSKTK"
     gene            12484..13902
                     /locus_tag="ACOIAS_000888"
     CDS             12484..13902
                     /locus_tag="ACOIAS_000888"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HGZ2700607.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            13917..15125
                     /locus_tag="ACOIAS_000889"
     CDS             13917..15125
                     /locus_tag="ACOIAS_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700608.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(15199..16587)
                     /locus_tag="ACOIAS_000890"
     CDS             complement(15199..16587)
                     /locus_tag="ACOIAS_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HGZ2700609.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            16928..18262
                     /locus_tag="ACOIAS_000891"
     CDS             16928..18262
                     /locus_tag="ACOIAS_000891"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HGZ2700610.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(18404..19321)
                     /locus_tag="ACOIAS_000892"
     CDS             complement(18404..19321)
                     /locus_tag="ACOIAS_000892"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HGZ2700611.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            19529..20413
                     /locus_tag="ACOIAS_000893"
     CDS             19529..20413
                     /locus_tag="ACOIAS_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HGZ2700612.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            20626..22467
                     /locus_tag="ACOIAS_000894"
     CDS             20626..22467
                     /locus_tag="ACOIAS_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HGZ2700613.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            22772..23479
                     /locus_tag="ACOIAS_000895"
     CDS             22772..23479
                     /locus_tag="ACOIAS_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700614.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            23473..24783
                     /locus_tag="ACOIAS_000896"
     CDS             23473..24783
                     /locus_tag="ACOIAS_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2700615.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            24900..25583
                     /locus_tag="ACOIAS_000897"
     CDS             24900..25583
                     /locus_tag="ACOIAS_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700616.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            25576..27963
                     /locus_tag="ACOIAS_000898"
     CDS             25576..27963
                     /locus_tag="ACOIAS_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2700617.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAIYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            28243..29385
                     /locus_tag="ACOIAS_000899"
     CDS             28243..29385
                     /locus_tag="ACOIAS_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HGZ2700618.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            29404..29928
                     /locus_tag="ACOIAS_000900"
     CDS             29404..29928
                     /locus_tag="ACOIAS_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGZ2700619.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            30028..30846
                     /locus_tag="ACOIAS_000901"
     CDS             30028..30846
                     /locus_tag="ACOIAS_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HGZ2700620.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            30924..31235
                     /locus_tag="ACOIAS_000902"
     CDS             30924..31235
                     /locus_tag="ACOIAS_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HGZ2700621.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(31362..33365)
                     /locus_tag="ACOIAS_000903"
     CDS             complement(31362..33365)
                     /locus_tag="ACOIAS_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGZ2700622.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYENMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITYPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(33518..34156)
                     /gene="recX"
                     /locus_tag="ACOIAS_000904"
     CDS             complement(33518..34156)
                     /gene="recX"
                     /locus_tag="ACOIAS_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGZ2700623.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            34432..35121
                     /gene="nadD"
                     /locus_tag="ACOIAS_000905"
     CDS             34432..35121
                     /gene="nadD"
                     /locus_tag="ACOIAS_000905"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGZ2700624.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            35122..35694
                     /gene="yqeK"
                     /locus_tag="ACOIAS_000906"
     CDS             35122..35694
                     /gene="yqeK"
                     /locus_tag="ACOIAS_000906"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HGZ2700625.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            35811..36161
                     /gene="rsfS"
                     /locus_tag="ACOIAS_000907"
     CDS             35811..36161
                     /gene="rsfS"
                     /locus_tag="ACOIAS_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGZ2700626.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            36515..38935
                     /gene="leuS"
                     /locus_tag="ACOIAS_000908"
     CDS             36515..38935
                     /gene="leuS"
                     /locus_tag="ACOIAS_000908"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGZ2700627.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            39771..40439
                     /locus_tag="ACOIAS_000909"
     CDS             39771..40439
                     /locus_tag="ACOIAS_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGZ2700628.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(40757..41029)
                     /locus_tag="ACOIAS_000910"
     CDS             complement(40757..41029)
                     /locus_tag="ACOIAS_000910"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2700629.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(41559..41681)
                     /locus_tag="ACOIAS_000911"
     CDS             complement(41559..41681)
                     /locus_tag="ACOIAS_000911"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700630.1"
                     /translation="MTFVERCYKYIDMNKNNWSPYTVTNTKSWVKNYIEPFLRI"
     gene            42081..43451
                     /locus_tag="ACOIAS_000912"
     CDS             42081..43451
                     /locus_tag="ACOIAS_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HGZ2700631.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
BASE COUNT        16770 a         4803 c         7445 g        14504 t
ORIGIN      
        1 acatatagct aatcaaagaa atgattttct acaaaaacta tctactaaat taataaaaga
       61 gaatgatatt atttgcatag aagatttaca agtaaagaat atgattagaa atagtaaact
      121 atctcgctta atttcagatg tatcatggtc agaatttatt cgtcaattag aatacaaggc
      181 taattggtat ggtagacaaa ttgtaaaggt aggtaaattc tttgctagtt cacaaatatg
      241 taataaatgt gggtataaaa acgaggaagt taaggactta aatataagag aatggatttg
      301 tcctagttgt aatgaaactc acgatagaga tataaatgca agtataaata tattaaaaga
      361 aggattaaga ctaataacga ttcaaaataa ataacatatg taagaaccgt aggaactacg
      421 gggatagcct gtggagaatt agtaagacat attttagtat gcaaaattct atgaagcagg
      481 aaccctgtga ctttagtcat gggaggttca gtttatttca aataatattt tacgtaatcc
      541 atctatgtta attggattaa tagcagcatt tggtcttgta ctacagaaga agaaagcatc
      601 tgatgttata aaggggtcac tacttgcagc tttcggaatt ataattatgg agactggaac
      661 aggtatgtta gttagttcaa tagcaccaat taatggagct ttccaaagta taagtggtgg
      721 tgcagtaaca aaaggtttaa gtgatgttac ttttactgca agttatggtg gaattatagg
      781 tttagcaatg tttgcgggtt tagctattca cctaatgata gcaagattta ctcctattaa
      841 gacaatcttc ttaacaggtc acatgcttta ttggttccca ttcatatttg tagcttcagg
      901 agttgaagct ggattaaatg gtggagtatt aataactttt gcagcagtat tatcagcact
      961 ctattggtca ataatgcctt ggttattaag aaaatatgtg tttgatgtta taggagatga
     1021 ttcttttaca ttaggtcatc catctggttg tttagcactt atatcaggat ttgttgctaa
     1081 attagtagga aataaagaaa aatctacaga agatataaac atacctcaaa gtttatcatt
     1141 ttttagagaa atatctataa gtggtggaat ggcagtgttt ttaatgttta tagttgttgg
     1201 gatagtaata ccaggtgcgt ttaaagatag taatgagttt attggaacgt atgcaataaa
     1261 gcaaggtctt tcttttggag caggtcttat aataatgctt catggggtaa gaatgctaat
     1321 aaatcaaata atgccagcat ttcaaggtat atctgaaaaa gttgtgccaa atgcactacc
     1381 tgcttttgac tgtccaatat tatttaatta taaaccaaat gctgtattaa taggattctt
     1441 agttgctatg ctaacatcta cagctatgat tttaatatat aactacttta atgtatttaa
     1501 tttgctactt ataccacttg ttataacttc attctttgag tgtggagcag cagcagtaat
     1561 aggagaatca caaggtggta tacgtggagc tataataggt acagctatag ctgcaatatt
     1621 tatggtagtt ttagtaggaa tatctgctgt aatttactca ggaactattc aaaattggat
     1681 actaatattt ggaggtaatg acctttcatt atttggtata atcagtaggt ttataggttc
     1741 actatttgga ggaatattat agatatgaaa tttaaataca tagatgagat aagaagatta
     1801 attgatataa tagaaaacga agaaaaagtc aatatggaaa aagcagtaca aatctttgtt
     1861 gaagcaatag aaagtaaaaa tgctatattt tcatttggag cttcacatgc aggaatatta
     1921 acagaagaat tattctatag agcaggtggc tgtgttctta taaatccagt atttgcaaaa
     1981 tctctaatgt tagatacagc gcctataact cacacaagta agatggaaag attagaagga
     2041 tatggtacag ctctagctga taaaactcca attaaaaaag gagatgtagt tatagttcat
     2101 tcagtatctg gtagaaatcc agtaagtata gagtttgcta tgaaagctaa ggaaaataag
     2161 gcaatagtaa tatgtataac taatttaagt tactcaaaaa gtgtatcttc aagacattct
     2221 tcaggaaaaa aactttatga agtatgcgat ttagtgatag ataatcatgg tcaaaaggga
     2281 gatgcctgtg tagagataga tggattaaac caaaaagtta gtccaacttc tactgtaata
     2341 gcaactactg tgatgaactc tattatagct caagctactc aggaattagt taataaaggg
     2401 ctaaagaatc ctccgatttt ctatagtgcc aatattgatg gtggagatga gttaaataag
     2461 aagatttttg atgaatataa aagtgtaatt cattatgaat attaaggtta aaaaaatatg
     2521 ttaatgaact ttgaagttta tcaacaaaga atattaataa tatataataa atgctatcat
     2581 ttaaaagctg taggtaaaaa tctacagctt tttattattg tagattaaaa agatgcgtaa
     2641 ttaaatttta aattggagaa aataaattgg aaggtatcaa ttataaatat tagaaacgtt
     2701 gtcatagaaa aaaactatat taaaaaaaag tacatagaaa aaactgatta gacaataaat
     2761 acatgctttg ataatatttt aaatggaatg aaaatgtgta gaattataat gaaaaaagaa
     2821 aataaaaatg aaaaaagaaa ataaaatgga gggaataaaa gtgggtatgt taaaaaaatt
     2881 atgttcacta gctatggtag caattatgac tacaacactt ataacagggt gttcaagtgg
     2941 aaatggaaaa gattcaaaaa aggatggcga aaaagaaaaa ctagttcttt ggatgccacc
     3001 agtagaaaat aatatgaagg aagtttggga ccctatttta gataaatttg aggaaaaaaa
     3061 taactgtgaa gtagatttac agataattcc ttgggataat tattctgaaa aatttgcaac
     3121 agctataagt gctggcgaag gtccagatgt aggatatatg tatgcagaaa tgtatccaca
     3181 gtttatagaa agtggagcag tagaagattt gactaactat gcaactaaag aggataaaga
     3241 gcaatctatt tatataaaaa catctgaaat gatgggtgga atgtatggta tgccattcca
     3301 agcagcaaat ccaggggtat tatactataa caaggatatt ttagagaaac taggagaaaa
     3361 acctcctaaa acatgggaag attttaaaag aatttgtaaa aaagcaacgc aagatactga
     3421 tggtgatgga aagatagacc aatggggatt agctcaggga tggggagcta aaacttttgg
     3481 taacttaaac tggaactggt atccttattt atggcaagct ggtggagata tatttaatga
     3541 tgatttaaaa tcggttaaat ttaatgataa gagtggttta gaagcagcta atttcttaaa
     3601 agaattacaa gcatatgtgc ctgaagattc tttatctaaa gatagtaatg aaatgataga
     3661 aagcgtattt ggaccaggga aagcagcatt tacaataatg ttatcttctg ctgcaacaag
     3721 tgtatttgat gaatcattcc cggatttaaa ttggggattt gtaactggac tagaagataa
     3781 gaaagcagca acttttggag cagtagacca tttatcttta atgtcatctg ctaaaaataa
     3841 agaacttgca tacaagttaa tacaacatat gttaagtgtt gaatcaatga cagaatttca
     3901 taaagcaata ccaagagcac caataacaaa aggtgaacca tatcaaggtg atgaaagatt
     3961 caaagaaatg gtggaaaatg acaaaaatgt atatagacca ttagtagtag gacctcatgg
     4021 agttgaaata tatgaatatt tatggaaaga acttcaaact atgatttcag gagataaaac
     4081 tccaaaacaa gcgttagatg atgctgcaaa atattcaaat gatttattag cacaataaaa
     4141 gtttaaagct gtaatatgtt gtaaaggact ttacagatta aaattagcat tgtatttata
     4201 aatacaatgc taatatttat attggagagg ataaaatgga aaacttagtt agagagtttg
     4261 aagaaaattc aaaaagaaaa aaaacaaaat taaatattaa accatatatg tatatattgc
     4321 cattgggaat aattttagtg gcgttttatg tgataccaat aataatgtca atatacttta
     4381 gttttacaaa gtataatatt attagtccag ctacttttat aggtttagaa aattataaaa
     4441 aattatttac agatgaaaca ttaaaagtat ctattataaa tactattaaa tttactgttg
     4501 ttgtagtacc ttgccaaact atattatcat tgattttggc tgtttggatt acaggaaaag
     4561 gaaatagtaa aatagcttca tttgctaaag gtgctatatt tataccagta ttatcatcta
     4621 tggttttgat aggtatggta tggagggctt tattgaatgg agaaggttct attatatatc
     4681 aggtattagg tgcctttgga atagaatcat ctaaactttt aggagatagt aagacagcat
     4741 tacctacact tatgtttata tctatgtgga aaaatatagg gtattttatg gtaatttata
     4801 tttcagctat aatgaattta ccaaaacact gctatgaagt agcgaaggtt gatggggcta
     4861 caaaacttca agaatttata aaaataactg tacctttatt gaaaccaact acaataatgg
     4921 ttgtattttt agggtcgata tggtcattac aagtatttga cttaatttat actgtgacag
     4981 gtggaggtcc aggtatatct actatgagta tagttatgca tgcatttaac ttaaacttta
     5041 aaaactttaa ttctggatat gcaatgactg tagcaaacgt attatttttg ttaatagcag
     5101 tagtttcgat tttacaaaat aagcttgtaa gacgagacaa ttcagatttt tagaaaggta
     5161 ggtataatat gaaaacagca agaagattat taagtgatgg tttgctttta tttatagcgt
     5221 gtgcaagttt ggtacctttt atatacatgt tgataatatc attgaagata acatataact
     5281 catatagttt ggatatatct ttttcaacag taacccttca aaactatata gatattttta
     5341 ctaaaaaagg atttgcacaa tattttttta atacagcaat agtatctttt tcaggtgtat
     5401 tattaaattt agtatttagt acattagcag gatattcttt tgcgaagatg gattttaaag
     5461 gtagtgataa gttattttta tttatgataa tgactttaat tataccttcc caagttacaa
     5521 tgataccact atatataata atgaaacact taggatggat aaatagttat cttgcactta
     5581 ttatgccaat accaactgca tttggcgtat ttataatgag acaagctatt ttaggagtac
     5641 caaaagagtt attagaatca gcaaagatag atggttgttc ggattttaga attttaatac
     5701 aaatagtgtt gcctttaatt aaaccagcat taattacact agcaatattt acttttatgg
     5761 gagcatggaa cgagtttatg tggcctctta tagctacaac taaagataca atgagaacat
     5821 taacagttgg attatctact cttaagacat tccagataac taattatgga cagatgatgg
     5881 caggggctac aataacattt ttacctccat ttattttcta tttgatttta cagagtaaat
     5941 ttgttgaagg tgtgtctttg tctggtataa aaggttaaat agtctaattt aataatatat
     6001 aaagaaactt atataaatat ggaggaattt aaactatgga aatttcatat gtactgaaag
     6061 attgcacatt caaacacaat aaatactcag ttgtagaagc tgaaacttgg aaagataagg
     6121 atttggatat tgtagtagct aaaggtgaaa aatttgcatt tcaaattatg ttaaaagcaa
     6181 cagaagaatt taattgtaca attgataaaa gtagtgctat atcatggaaa ggcctgggaa
     6241 atagagtaag gttagcatta aatatgccta atagtttaga aaataatttt agtataaata
     6301 tattaggata tgttcaagat gatacaaagg catttgtgaa tgatgctatt ttaagggata
     6361 aagatgtgtt agtagagcaa tatttgcctc aaacattttg gattgaaggt caagtaccag
     6421 aagattttga agaaaataac cttgatatag gtattgatat atttaaaagc tttggatatg
     6481 aagacgaaga aaaagtttgt acaattgatg ttcctgtaaa agtaagaaat gttgtgctaa
     6541 agcctttaga tgaaagcaag ttctttttag atttatggca acatccaagc tgtttagcta
     6601 gaatgtataa agttgagtta tggtctgata tacattttga aatagtcgat aactatttaa
     6661 aagaattagc atctttagga gaaaaagttg caacagttat agtttcagat tatccatggg
     6721 cagggcaaag ttgttataaa gtttataaaa atccatcaaa cttatatgaa tataacatgg
     6781 taagtgtatc aaaaggattg gatggaaaga ttaaatgtaa ctttgagagt atggataggt
     6841 atataagcat agctgataag tataaaatgg ctaaggagat agatttattt ggtctattag
     6901 gtaattggtg tgctggtgag tttggaaatc ctgtagaagg ttataaggac cctataagag
     6961 taagatattt tgatgaaaag gataaagtat ttaaatttat aaacaataca aatgatttaa
     7021 aagaatatat aggattagta ttaaatcatt taatagagtg tggattatgg gacagagtaa
     7081 ggattatagc tgatgaacca aataatccag aagtagtcaa agagtgtata gaatttataa
     7141 attctactgt aagcactcat caagttaaat ataaatcagc aactcacgac caaaactttt
     7201 tagacagagc taaagatgaa attgatgata tgtcaataaa tttaaaactt actatacaaa
     7261 attataaaga tatagaaagt cttaagaaaa aaatcaatga caagggtggg atattgactt
     7321 ggtttgtatg ctgttttcca gagaaaccaa atagtttttt aagttctcct tttgtagaaa
     7381 atagaataat aggatggtat acttattact ttggtcttga tggattttta agatgggact
     7441 ataatttatg gacagaagac ccttggaaag attcaagtta caaattccct atttggaaag
     7501 ctggagatat gttctttgtt tatcctggaa aagatttaaa gccagttaga agtgtcagaa
     7561 tggagaattt aagatttgga atacaggact ttgagttatt tacaatgtta gaaaaagaaa
     7621 agggaagaga gtatatagaa gttgagctta tgcaagaatt attaaataaa aaagaaaatg
     7681 ctgaaattaa aggttttggt gacatagaac taggatattc acttgataat tggggatatg
     7741 ataaagttaa aaaatatgta ttagagttgt tagacagagt ataaaaaata taagagtgtc
     7801 tcaaaatgaa ctttttagtt catgaggcac tctttttgta tgaaatcaat atagtttcat
     7861 tgtttcaaca atgaaaaata ggattatttc tattctaaga cagcccttta gttttttagt
     7921 tgtattcaat gttttaaaac taactataaa cgctataaag tttataaatg acaataatta
     7981 tatataacat gatgtacttt aatttgctat aatagggatt gaataatatt atatggaaga
     8041 taacattgtt tggggggatt aatataaaaa gaataggtat tattataatg ttttttttaa
     8101 gtatatcttt tattggattt tatcttgtaa taagagataa cagtgataca gttaagatgc
     8161 aacgagggat aaatataggt aatgcactag aatcacctaa ggattttccg tgggatgtaa
     8221 aaatgtcaaa taagtttttt gatgatataa aagatgcagg ttttgacaca gtacgtatac
     8281 cagtaaggtt ttctgattat acgtcagatt ctgataattt taaaattgat gaagaatttt
     8341 ttaaaaagat tgataaatat gtagattatg cattggataa agacttaata gttgtgttgg
     8401 atttacacca ttttgaagaa attatgaaag aaccaagagt tcataaagaa aagtttttaa
     8461 aaatatggca acagatagct aataggtatc aaaaatatga taaaaaattg gtatttgaat
     8521 tattaaatga acctaaagaa aatttatatt cacagttact aaacgaatac attgaagaag
     8581 caataaagat aattagaaaa actaacccta aaagaactat tatagtagga ccatataatt
     8641 tctatcaaat tgattatttg aatgaattaa atattcctaa agattctaat atagtagttt
     8701 cctttcacta ttacgagcca aatgattttg catttcaagg aaatatatat cataagggat
     8761 ttgagcactt aagcaatatt acttgggaag gaacaaacga acaaatggat tatttaaaaa
     8821 aaaggtttga tactgtggaa aattgggcaa ataaaaataa ggtaaaaatc ttcttgggtg
     8881 aatttggaat aacaaaagag gcaccagaag cttcaagaag agcatggata aaagctgtaa
     8941 gagaagaagc tgaaaaacga aacttttcat gggcttattg ggagcttgca tctggatttg
     9001 gtatatacaa tcaaattgaa gggacttggg atagagatat attaagtgca ttaatagaaa
     9061 agaggtgatt ttatgaagaa aaattcaaaa aagatactta taactttaac tattattact
     9121 aatgtcatat atatattgtg gagaattttt tacacagtac ctaaagaaga agggaagttt
     9181 gccttaatat gtgccataat acttcttttt gtagagataa tgggcatgat ggaaatgttt
     9241 gtgcattatt atggtatgtc aaatattgag taccctgaaa aaccaatcat aagtgaagaa
     9301 ctttatccac atgtagatgt attcattgca acatataatg aatctgttga tttggtgaga
     9361 aaaactgtaa atgggtgtat tcatatgcag tatccagata agaaaaaggt acatatatac
     9421 atatgtgatg atggaaatcg tgaagaaatg tgtattctag cagaaaaaat gggtgtaaat
     9481 tacataacta gaactgaaag agaaggagca aaagctggaa atttaaataa tgcaatgcaa
     9541 catacaaatt cccctttgat tgctacattt gatgcagata tgataccaat gcatgacttt
     9601 ttaattgcta cagtaccata ctttttgaaa aatgagcaag ctaaaaaaga tggagaaaaa
     9661 gaggagtatg aaaaggttgg ttttgtgcaa actccacaga gtttttataa tccagacttg
     9721 tttcaattta atctgcactc tgaaggaagg attccaaatg agcaagatta tttttacaga
     9781 gacattcaat tggctagaaa taggacaaat tctgtaattt atggtggtag taatacagtt
     9841 atttcaaggg aagcacttga agaagttgat ggattttata cttattctat tacagaagat
     9901 tttgcaacag gtatcttaat tcaaagtaag gggtatagat gttatgctat tccagaagta
     9961 catgcatcag gtttatctcc tacagattta aaaagtctta ttaagcaacg tgaaagatgg
    10021 gcacgtggat gtatacaaac tggtagacgt ctaaatattt tgtttaggag aggtttaggt
    10081 ttttggcaga aaataagtta tatttcttca attacttatt ggtatgcaag tataaaacgc
    10141 tttgtatata taatggcacc tattttattt tctgtgttta atgtgattgt agtaaaatgt
    10201 acgttacttc aagtattggt attttggttg ccaatgtata ttttaagtag tttatctctt
    10261 aaaatatttt ctcaaaatat aagaaatact agatggacca atatatatga aactataatg
    10321 tttcagtctt taatgccagc agtaatactt gaaacgtttg caatatctaa aaataaattt
    10381 tcagtaacaa ataagagtaa attagaagaa aatagaatgt ataagttttt acagggaata
    10441 ccttatttta tttacatggt cttatctatc ataggaattt taaaaatgtt tgtagcaata
    10501 tttaaaatga gttcaatgac atattcagtt gtattatttt ggttgattgg aaaccttttt
    10561 aaccttgtta tggctacatt atttatatca gggagacagc agttaagaaa gtctgagcgt
    10621 tatattgctg aaattgattt taagttaaag caaaattctt atgttctttc ttcaaaaaca
    10681 attgatatat cagaaaatgg gtttgcattt ttgctagaaa atccagaata tatttcccca
    10741 gaagaagagt ttgaggtaga gtttagagaa aaatctggaa atgaaatgta tatagctaat
    10801 atgaaagcaa agattgttaa tgttgtggaa gtaaattcaa agtggaaata tgcagcttat
    10861 attacacata ttgaagatag tgaaatagat aattggatgt gtattgttca cgatagaata
    10921 cctactttac caatgacaat atctaatcaa ctaggtttct ttgatgattt gcaaattaat
    10981 gtaaaaaaac gtattgaaaa aactagaaca ttatcacgaa gaagtccaag aatcaatatg
    11041 aattttcaga tggatataaa aaatacaggt aaattaagaa tagtgaattt taattatcaa
    11101 tatgtattat taaattttga gaataaaagt atttatccta aagaaatagc attggaaatt
    11161 aatgaaaata ttgtgttaga atgtgatttg tgtgagggta aaattgatga aagaggtatc
    11221 ttatataaag taaataacat tgacagcata atgcaaaatt tctttttaag agatgaaatg
    11281 atggattgga tattacaaaa taagaaaatt cttgattcaa agcctagtga aaagaaggaa
    11341 aaaagtatag atgagtttga acccatggaa tatatttagg agagatgaaa tgaagacttt
    11401 aaaagtgttt ttttgttcta caaaatatct tattttggtg tttatttttt ttataaatac
    11461 tacttttata agttcagcaa caagtgataa aattttagat ttatccttta aaaagataga
    11521 gactgactta agttctaaga ttacatatga ggatacaggt gtaaaaatag aaacggatag
    11581 ttctaaaagc gataaggaac gttatttata tatttaccaa aatataaaag aaaattggag
    11641 catgtataat aatttttata ttgaaattca aaataaaaat aaaagttctc agagaataaa
    11701 tttaagcata caaagtaaaa atatgcttga atttagatta aaagaaggta gtgaagtatt
    11761 tttagaaggt aaaaatataa tatattcaga taaaataaaa gaaggatgca tagaagtacc
    11821 aggtgaattt gaaggcaaaa tttatgtgaa ctttaatagc cttataaatg aggaaagtaa
    11881 tgttgtgctg gactcaaata tgctttcaaa cattgttagt tggggaatta cgtttattcc
    11941 atctgatgaa gaacataata ttgttattat aaaaaagata tctttattgt ctgaagagaa
    12001 attaagattt ttaaataata ttaaaataat tggcgatgaa gaagtacaaa taccagtatt
    12061 gggtcaaagt ataagtcagt atgaggtatt aggtcttaaa agtgattcta aaattaagta
    12121 ctctcttatg ggaaaacaag ataatgtgag tatatctcaa aacggtaaat taacattaag
    12181 taataaagcc aaacctggac aaattatttt acaagttaat gttgatgata agtttaaaat
    12241 tggaaagaaa ataacattaa ctgaatcttg gagtattaat aaaaaagata aggatggtgt
    12301 tccatataca ttagtatctc ctgagcaatc accaacagta caagatatga aaaaaataaa
    12361 ttttatgaat aatatcataa cgtttgttcg aattttattt gtatctctag taataatatg
    12421 ctttggaata tatttatatt ggaaaaaatg ttcaaaaact aagtaaagag aggaggttag
    12481 attgtgttat tttcaagttt aattttttta ttttattttt tacctataac tttggtttta
    12541 tattatgtat ttaggtttaa ccgaacgata cagaacatga tattgcttgc tgttagttta
    12601 tttttttatg cttggggaga gccaaaattt gttgttatca tgatagtttc cataataatg
    12661 aactatatat ttggtttact ggtagataga tatagggaaa gtaaaataaa agtaaaagtt
    12721 tttttagttt tgatgtgtgc atacaatata ggtgttttat ttatatttaa atatctagct
    12781 tttgcgctaa gaaatattag tgaacttatt agtactgaat tgactatacc taatattatt
    12841 ttacctatag gaatttcatt ttttacattt caaggaatgt cttatgttat agatgtatat
    12901 cgtagacatg gagaagtcca aaagaatcct ttttatgtgg gactgtatat agcatttttc
    12961 ccacaattaa tagcaggtcc tatagttaga tatgagagtg tggcagaaca aattctcaat
    13021 agaaaagaaa cttggaataa attttctatt ggtacttgca ggttcataac aggtctaggt
    13081 aaaaaagtat taatttcaaa taacatggct atagtagcag actatattta cactatgaat
    13141 agtcaaggag aaatagctgc atcacttgca tggcttggtt caatagccta tactctgcaa
    13201 atattctttg atttttctgc ttattcagat atggctatag gcttaggtct tatgtttggt
    13261 tttaaatttg aagagaactt taattatcca tatatatcaa aatcaattag tgagttttgg
    13321 agaagatggc acatttcttt gggaatgtgg tttaagagct atatatactt tccgcttgga
    13381 ggttctagag tagcaaataa agatattatg attagaaata tgtttatagt gtggttattc
    13441 actggaatat ggcatggagc agaatggaca tttgtaattt gggggatttt aaactttgtt
    13501 ttcctaatta tagagcgatt tataatgttt gaaaagattg aaaatcacaa ttttataaaa
    13561 catatttatg ctttattagt agttaatttt ggatgggtat tgtttaggtc acctaattta
    13621 aaagaagcat ataattattt taaagctatg tttggaggca atggagctat atggagtgat
    13681 tatacatata tgtttttaaa agaatactgg gttttcttta tatttgcatt tatttttagt
    13741 gtacctattg caaagaaaat caataagttt gttgttgaac aagctaaata tagtatgata
    13801 tttaacgtat tttatccaat ctcagctatt ttattgtttt ttatttctgt gacatattta
    13861 gtaactggaa gctataatcc atttatttat tttaattttt agttgggagg aaaagtatga
    13921 atagattttt taaagtaaga ttaataactg caattatgtt cttaattatt ttattcgctt
    13981 tctctatctt taatgtttgt tattctggaa aagatacgat acaggaaata aaaaattcaa
    14041 tagctgaaaa aagtagttta aaagatacaa tatcatctat tgataataca gtgaatgaca
    14101 aagtaatata caaaaatacg ttcattgaaa cttatggcta tttacaaaaa ttaatgggta
    14161 aaaatgagtt ttcaaaattt agtgttgtaa aagatacttc tggaaagatg cattatacat
    14221 attttgctaa agctccaaat ccagttggtg tgatagcaga tagggtaaga aaattttctg
    14281 atgaaatgga aaagcaaaac actaaattgg tttacctaat gactccagat aagtatataa
    14341 aaggagtgac aaaatttcca aaaggaatac cttattctta tagtaatgag acagcagata
    14401 attttttaaa tcaactaaaa aagtataatg tggactatat tgattttaga gaaaatattt
    14461 taaagagtgg tattccaaaa gatgatttat tttttaaaac agaccatcac tggaaagtag
    14521 aaacttcttt ttgggcattt ggagaattag ttgagcaact taataaaaaa tacaatatga
    14581 atttagatga aaatcattat tatagagata aggacaatta taattcaatt gtatatccta
    14641 agtcatttct tggttctatg ggaagaaaga ctggtatctt atatggtggt atagatgatt
    14701 ttacattaat ttatcctaaa tttaagacta attatactta ttatacagat tctaaatctc
    14761 aaaaatttga attaaaagga aggtttgaag agagtttaat attatcatat ccttttaatg
    14821 cagaccttga tttaatggat ggtcaatcag ataagtattt tacatattta ttggggaaca
    14881 gaccattagt taaaataaaa aatatagaaa atccagatgg attaaaagtg ctgtttgtta
    14941 aagattctct aattgtacca acagcagcat ttttttcctc tgtttgttca tctatagata
    15001 tgatagaccc tagatattat gatggagata tactagaata tgcaaaatcc catgactatg
    15061 actttgtttt tatatctgta tatccccaaa atcttacaaa agaatttttt ccgttttgtg
    15121 agtaaatttc taaaaaagtt ttctgataaa ataaatggct aactggtaaa aagttagcca
    15181 tttattttga gtgttattct aagaaatatt ttttttacta aatacttctt tatctatttt
    15241 tccaagtgat tttcctacag taattgatga taccatagca ccattaacat ttatcatagt
    15301 acgtcccata tctagtatag ggtctattgc aagtatacca ccaactaatg gaaaataaga
    15361 accaagacca actccagaga taacaactga aacggccatt gtagcagttc ctggaagacc
    15421 agctattcca aacgaactaa ttgttataac aattaacaac ataacataaa agctcatatc
    15481 catgtttact ccagccatat tggcaagtgt tacagccatt aaagcaggat atatacctgc
    15541 acaaccattc atacccatat ttgcacctaa acttgctgta aagcttgata caccctcatc
    15601 aactccgtga ttttgtacta gactttctat cgttacaggc aatgttccta aactagaacg
    15661 tgaagtaaat gccagaagta aagcttcagt tgaatttttt atataagtaa tgggatttag
    15721 accatttaaa gttataaaaa ttaaatgtat aataaataat attgcaactg atatataaag
    15781 agctaatata aactgtaaaa cagatactat tgaactaata ccttgtgaag ttattgtatt
    15841 tgcaagcaga gcaaccacag cataaggcat aaatttaata acagtcatag acacacttac
    15901 aattattttg taagatgctt ctataaaatc tataaatggt tttattgtct caaaatgttt
    15961 tttacttaat ctctttattg aggtagctat aaatgtagca aatattattg tagctacaat
    16021 atttccattt gccatagcca ttacaggatt agatggtagt aatcctctta aagtatccac
    16081 aagagagttt atttctttaa gttcttgatt ttcaactaaa gtaggcatat tagctcctac
    16141 acctaacttc attatatttg caacgataat tccaacaatt gcagctatag cagtagtagc
    16201 aagaagcata cctaaagatt taaatgtcag agttccaaga ttgttatctt tcatatttat
    16261 aattactttt ataatggata agaaaactaa aggtactact aacattttta ataagtccat
    16321 aaatccagaa ccaaataggc tataccactt acttacttct tgtagccaag tgacttgagt
    16381 tgtatcatta ggaaaaccag caaaagcttg tattatgatt cccaatatga gtcctagtac
    16441 tgtagcatat attgttctca atgaaaattt tatttttttc ttttcaaatt gtctaacaat
    16501 aaagaacgta cctagaagta atattatgaa aaaaattgtt ttaaaatctg taatcataag
    16561 aaattgtgat aaaaatgaac tttccaatat ttttctcctt ttatttaaaa ataaattttt
    16621 ctaagtttaa atgatatcaa tatattaaaa aaatgtcaat attatatcac aatttactta
    16681 aatagagtaa aacaagttga ttttgaaaaa tgtgattaag tacagaaaaa tagggtataa
    16741 taaatgttta aagaatttga attgtggtaa gaaaataaaa aatagctttt ataagatttg
    16801 atttcaaatt aaaatatgtt aaataaataa ctaaaaatta ttataaaaaa tgttttcata
    16861 gaatatatat caaatgaaaa gcttaaaata agtgaagaaa ttaactagac atcaaaggag
    16921 agataaaatg agagtaataa taattggtgg tgttgcagct ggtatgagtg ctgcagcaaa
    16981 attaaaaaga ataaaacctg agtatgaagt tgttgtttat gaaaaaactg aaatagtatc
    17041 ttttggtgct tgtggattac catactttgt tggtggattt tttaatgatg ctgatgaatt
    17101 attagcaaga acaccagaga aacttagaga agctggaatt gatttaaata tatttagaga
    17161 agtcgttgag gttgatagtg aatctaagaa aataaaagta aaaaacataa aaacagatga
    17221 aatatatgaa gattactatg ataaattgat gatagcaact ggagcaagaa gtattatgcc
    17281 tcctataaaa aacataaaat tgaagaatgt ttctacatta aaaagtttat atgatggaga
    17341 atatctaaaa aaacttttaa gtaatgaaga taataaaaga gtgacaataa taggagctgg
    17401 ttttataggg ttagaagcag ttgaggcatg taagaaatta ggtaaagatg tagatgtaat
    17461 tcagttagaa gatagaatat tgccacaagt atttgataag gaaatgacgg atgtattaga
    17521 agaagaaata aatagacata atgttaattt gcacttagat gaaatggttg tagaactttg
    17581 tggagaagat aaagtagaaa aagttataac aaacaaaggg gaaatagata cagatgtagt
    17641 aataattgct actggcgtga gacctaatac cgagtttttg tcaaatacta atataaaaat
    17701 gctaaaaaat ggagctatag tagttgatga gtatggaaga acttctgtag aagatatata
    17761 ctctgctgga gattgtgcaa ctataaagaa tatagtaagt aatgaaaatg tatatgttcc
    17821 tttagctact ggagctaata aattgggtag gatagtagga gaaaacctag caggaagaga
    17881 agtatcttat caaggttcac tatcttcaag ctgtataaaa attatggata tggaggcagc
    17941 atcaactgga attacagaaa agcaagctaa agatttaggc ataaatgtca aatctaaatt
    18001 tatttctgat tataatcaaa ctcattatta tccaggaaga aataaaatat atgtaaagct
    18061 tatttatgat gcagatacaa aagttatttt aggcggacaa gtggctgggt ttaaggatgc
    18121 tgtccaaaga gcaaatgtat tggcagctgc tatctttgga aaaatgacca caaatcaact
    18181 tggaatgtta gatttatgtt atgctccacc atttgcaaga acatgggatg ttttaaatgt
    18241 agctggaaat gtatctaaat aatatttaga ttatagctaa tatagttgac aagcaattaa
    18301 gggaatgaat ttacctaaac tatttttatc atggtaaaat tcattcctaa atttttattc
    18361 caaattttta ttccaaattt ttagaaataa ggattccatt aaattattca tcaaaattta
    18421 aacgcaagct taatctatct tctccaagga tagtattttc aaaaacatta gtagcatttt
    18481 ctaaatacat actaggtatg tctaaagaag gactaaaatg agtcagtaaa agttgtttga
    18541 catttccaag ttttgcaaga ttagcagctt ctctaaaagt catatgtttg tttctaacag
    18601 cttttgatat atctaaatca tcaccataca tggcttcaca tataaataaa tcactatctt
    18661 ttatgaaatc tggtattgat tctataggtc ttgtatcggt tgtaagactt atcttaactc
    18721 cctttctcaa ttctcctaag accatatctg gagtgtattg ttttgaatct aaaactatat
    18781 tttgaccttc ttggagtttg ttccataata ttttaggaac ttcgttttta gttgccttat
    18841 ctatatcaaa tttagggttt cttctaaaat taaaactgta tcctaaacac tctatagagt
    18901 gttctaaact gattgttgaa atttctaaat ttcttaaaac tttattatct aaagaaaaat
    18961 tgccttgagg gttttctata atttttaaag tgtatggtac atattctact aaatttctca
    19021 tagaacgtat acaatcgact attccaactg gaccaactat agttaaatca cttgtacgac
    19081 cactatttcc aattgttgat aaaagtccta aaagtccaaa tatatggtca ccatgtaaat
    19141 gggttataca aatcaagtct atatctttaa agccacattt ttttagcttc atagatacct
    19201 gagtaccttc accacagtca attagtattt ttctaccttt atagtttata aatacagagg
    19261 atagaaatct gtttggcata ggtacatttc caccacatcc aagtaaagtt aaatctatca
    19321 ttttttcccc tttctaaaat taacacaaat ctgttgtttg tttttattat gatatcctat
    19381 tataattatt ttaacattaa atataacaat aaatgtgtat ttgagtcaag aaatattgtc
    19441 aaataaagtt tttatataga aatgtataaa aatttggaaa acaaaaagtg aaaattatat
    19501 ttaggagcat ttaaaggagg aaaatttaat gaaagtatta aagaaaatta ctattttgtc
    19561 attaattaca ttaaccttag ttggttgtag taacggagaa gataaaaaag tggttgaagg
    19621 ttctaagaaa attcaaaatt ctaaaaaaga tgaacagcct ataatagtac ataaagatgc
    19681 aaatatattg aaagataggt atgagccacc aaaaggattt aaaagagttg atgtagaaaa
    19741 aggtagtttt gaatcatttc ttaggaatac taagctaaaa aattatggag agaaagtaaa
    19801 gtattatgat ggaagaacta aggaatcaaa aggtatttat gatagtgtat ttgatgttga
    19861 tattggcaat agagatttgc atcaatgtgc agatgcaatt atgcttatga gagcagaata
    19921 cttgtatcag aataaactat atgatgaaat aagttttgat tttgttgatg gttttaaggc
    19981 gaagtattca aaatgggcac aaggatatag aatttcagta aaagatagtg gttcaagctg
    20041 gtataaagca actgaatatt caacatctta tgagagtttt agaaagttta tggatatagt
    20101 gtttgcatat tcaggaacgt tatctcttga aaaagaattg gagcctgtaa aagttgagga
    20161 tatgaaaata ggagatgtgt ttatagttgg tggaagtcca ggacatgcaa gtataattat
    20221 ggatatggca gaaaatgaaa agactggtga aaaggtattt atgatagccc aaagctatat
    20281 gcctgcacaa caaacgcaac ttcttattaa tcgtaaaaat ccaaatttga gtccttggta
    20341 tagtttagat tttgaaggtg atttgacaat acctcaatgg acttttaaaa gagaccagtt
    20401 gaaaagattt taaaataata attttatata ataaaagaaa aatatagatg tgaaaaaatg
    20461 taattagatt aaaactaatt ggatatggac aaaattaaat tgatagataa atattaaaaa
    20521 ttacactaat taatcttact atagtttaat atttatctaa aaagttttat gtaaaatata
    20581 aattttatat taaagctaaa actaatttga ggaggctaat ttatattgaa gattagaagg
    20641 tatttaattt tggtaattat aattctaatt acaacaagtc agagttttat ttcttatggg
    20701 ctagagcgat ttgaagaaat aaccattttt cacactaatg atatacatgg aagatatgct
    20761 gaaggtgatg accatataca aataggaaat ctagccacat tgaaaaaaga aactccaaac
    20821 tcaattttag tagatgcagg agattgtttg catggacttc caatagtaaa tatggataga
    20881 gggaaaagtg caattgaatt aattaaatgt gctggatatg actatattac tcctggaaat
    20941 catgacttta attatggaaa agatagactg tttgagttat ctaaaagtgc taatttagga
    21001 gagaatactt taaaattttt atcatcaaat atttttgaaa atggaaaaaa agtttttgat
    21061 agtaatgata taaaagaaat agatggaata aaaataggat tttttggact ctcaacccag
    21121 gaaacaaaga gcaaaactgg atttaaaaat gtggaaggat tagagtttaa aaatcctata
    21181 gaaagtgcaa cagaacaagt aaatgaatta aaaggaaagg gtgccaatgt aatagttgca
    21241 atatgtcata ttggtacaaa tagtttaagt aagccaaata gtatagatat agcaaatgag
    21301 gttcaaggaa ttgatttgat tattgatgga cacagccaca ctaagtttga aaatggtaaa
    21361 caagtgggtg atacattgat tgtaagtgca ggacaatatt tagaaaatat tggcgagata
    21421 aaactaactt tggatactaa tgatgtagaa aatataaaaa tagaaaataa atcagttaga
    21481 ttaattggaa aagaagaagc attaaagtat aagcttgact ccgctgtaag taaaaaggtt
    21541 gatgaaataa aagaacaaca agaaaaaata ttagataaag ttataggggt aactacaaat
    21601 actctagatg gttcatatga aaatgtaagg acaaaagaaa ctaatttggg taatttaata
    21661 agtgacattt tactagataa aactaaggct gatatttctt tatttaatgg agggaatatt
    21721 cgagatacga tagaaaaagg caatataaca agaagagata ttgtagatgt atttcctttt
    21781 agcaatacta tagttaccaa agaacttaca ggagcacaga ttaaagatgt attggagcat
    21841 ggagtaaaac tttatccaga aaaaagttca gcctttttgc aagtgggagg aatttcatat
    21901 tactttaatc ctaaacaaaa agaaggcgaa aaaataacaa atattcaaaa agaaggaaag
    21961 ccattagatt taaataaaaa gtatgtagtt gcaacgaatg attatatagc atcaggtgga
    22021 gatgaatttc cttgtttttc aaaagagcca gtactaaagg aatttggaaa tttagagagt
    22081 gcagtcattg aatatataga gtacaaaaaa gaaatatcaa aaaatgtaga tggaagaatt
    22141 ggtattaaat ctaaagatga gttaataagc aagaatcatg agagtgattt gatattaagt
    22201 gaaaatgaaa aaaatatcaa tgataatgaa gagaatctat ctaataatat aaaaagctct
    22261 gatagtgata aaaataaaag cttagataaa acaagtgcta gagaaagttc taatctagga
    22321 atatctaaaa gagaagctaa agaaaaatct cctaaaacag gagatttagg tttttcaaat
    22381 agtataatta tatttatagt ttcaagtaca ttgatatgtt tattgaattt taatcaaaaa
    22441 gagttaaagg ataaaaagtc aaaataaaaa ttaatgagca aaaattagta gatagtaatt
    22501 taattttaga taatagtaaa cagcaaagga aattagttat ataaatacta atttcctttg
    22561 ttttatgttt taacatgatt acttttaaat aaaattgtat tttatgaaaa ataattacac
    22621 taaatggcat agtattatgg ggatttttag tttttattca agtaaatgaa atcatattaa
    22681 caacttctga tatatttaca aattgataaa aaaatgattt tattagtatg atataattta
    22741 gctagtatat atgatgatgg aggatatagg gatgaataat atattacttt tggaagatga
    22801 caaatcttta aatagaggaa ttagttttaa attaaaaaaa gaaggataca atgtttttag
    22861 tgcatttggt atagaagaag caaaaaatat tttcgcaaaa gaagagatat gcttaattat
    22921 ttccgatata ggtctacctg atggtagtgg ttttgacttt tgtgaggaga ttagaaagaa
    22981 aagtgatgtt tacataataa tgttgacagc tcttgatgaa gaggtggata ttgtcacagg
    23041 atatgattta ggtgcagatg attatatcac aaagcctttt agcttgatgg tattaatatc
    23101 aaaagtgaat gcgcttatga aaagagtaaa tacagtaaaa aattatactc tattagtttg
    23161 tgatgattta ttcttttatt atatagaaaa taaattaatt ataagaacag atgataagga
    23221 agaagagata attttaagta agacagagac aaaactatta aaatatttga tggaaaattc
    23281 tatgcagaca ttgactaaag agcaactgtt agaatcttta tgggatagta gtggtaattt
    23341 tgtagatgat aatacgattg cagtaaatat aagaagactt agacaaaagg ttgaaaaaaa
    23401 tccatctgaa cctaaatata taaaaacagt aagaggggtt ggatatatgt ggggagaaag
    23461 gagtataaaa aaatgctaaa aaatatgaac ctcaaagatg gtttaagtgt gaaagatgat
    23521 tatataatga gaagagttat tgtatattct gttttatctg tactaatagt aacccttgta
    23581 tttatacttt ttgaatatag cagattacat aaattgtatg aacatcaaat aaaaaacact
    23641 caaaatattg ctggaatatt gattgaaaaa tatcctaatg atgaaataga tatagtaaat
    23701 tctatttata attctgatta tagtaaggtg tctactggag aaaaggcatt taagaaattt
    23761 ggctatggat tggaaaataa aatgtcaaat gataaaaatt ttagcttata cttaaaatct
    23821 ttttttaagg aatcattttt tattttttta atgatgttta tagtagttat aggtataata
    23881 tattatttta taagatacat aaataaaaga ttgagtaaaa tttattttat agtagataag
    23941 atgtcacaag aaaactactt gaaggacact actgagtcag aagaaactgg ttatctagta
    24001 gaatataatg aatactttaa agaaggcata tttagtaaga taaataactg tctatatgag
    24061 cttaatagaa gtttaaagat aaagtttatt aaattagaaa aagaaaaaga aagtgtaaaa
    24121 tcattagtaa cagatatttc ccatcaatta aaaactcctt tagcatcctt aaaattatac
    24181 aatacattac ttatagaaga agaattagaa gaagatgaag ccaacgagtt cttattaacc
    24241 aataaggctt ctataaataa attagaaaat ttaataaatt ctttagtgaa catatcaagg
    24301 ttagaaatta gcatgataaa cataaaaaaa gaagataatg atataaaaaa tactattcta
    24361 aatgcagtaa aaagtgttac ccctaaggct aagagcaaaa gtataacatt aaatttaaat
    24421 gaatttaaca gtagaattat accacatgat aaaaagtgga ctgaggagag tatttttaat
    24481 gtattagaca atgctgtaaa gtatacacag gtaaatggag aaataaatat atctgtagaa
    24541 gaaactgcta atttctttaa aattataata gaggataatg gcatagggat tgaaaaaaat
    24601 gagtttaata atatatttaa acgtttttat agaggttctg ctgatgaaat tgaatctgta
    24661 gaaggtagtg gggtaggatt gtatttaagt agaaagattt tagaagaaca aggtggaaat
    24721 attatagttt cttcaaaagt aggaataggg agcaagtttt ctttgtttct tacaactatg
    24781 taatattaaa tgaaaggaaa atgtaagcct ctacatttat tatataagta taaacagctc
    24841 cagaaataaa aaattaataa aatatggagt aatataactt agaatgagag gataatttta
    24901 tgaaaatact atatactgaa aatttatcaa agcattatgg aaaaggagaa agcttagtaa
    24961 gagctttgga caatgttgac ttagaaataa atgaaggtga atttgttgca ataataggaa
    25021 aatcaggttc tggaaaaagt acacttttgc atatgattgg aggtcttgat atacctacat
    25081 ctgggaaggt gtatatagat aataaaaata tatttacatt aaaagaagaa gaacttgctg
    25141 tatttagaag aagaaaaata gggtttatat ttcaaagcta taatttaatt ccatctctta
    25201 atgtatggga aaatgtagtg ttgccaatcg gtttagatgg tagagaagta gatgaatctt
    25261 ttataaaaga acttttaaaa tcattaggac ttgaaaataa acacgacgtt ttaccaaata
    25321 cattatcagg tggtcaacaa caaagggtgg caatagcaag agcattagct acaagaccag
    25381 ctataatact tgctgatgaa ccaactggaa acctagattc taaaacgtct gatgaggtta
    25441 tgagtatatt aaaatctatg tctaagaagt acagtcaaac tctagttatg ataactcatg
    25501 atgacagtat agcccaaatg gctgatagag ttatattcat agaagatggc agggtgtcaa
    25561 aggttggtga taaaaatgat taatactact aaaatagcaa aaagtaattt aaagcaaaat
    25621 aaatcaaaaa gtattctaat aataattact ataatacttt caaccttaat gctttcttct
    25681 attggaattt atattgttga tgcaggagca tatcaaaaag aaaatactat aaagtattca
    25741 ggcaattatc aaggtatatt agcaaatgta gatgaaaaac aggctgatat attgagtaat
    25801 catgcagatg ttgaattaac tggtgaaatg aatggagtag gtgttgaaaa actggaagat
    25861 gattcaaata tttctctagc atacatgaat gaagatgctt tgaaattaaa tagttttgaa
    25921 tttatcaaag gaaaattgcc atcaaaagag aatgaaattg tgttagatag tggagcttta
    25981 aaggcacttg gttataaaaa taaagattta ggtgaaaaga taaaaatatc ttatgatgac
    26041 tataaaaatg ataaaaaaat tgaaaaagag ttcattataa gtggaatctt aaaaactagc
    26101 gaaatatctg aagcaggtaa atattattat gctattatat cagaatcata tatgagaaat
    26161 actagagata tgtcacaaga agattttaat atttatgtga aatttaatga taaaagtaat
    26221 ttatcaatag agcaagcaaa agaaaaatta gataaaattg ctaatgatat aggactggat
    26281 actattaata ctgctgtaaa tgaaaattat ataaatgctt taaaaccaga tatggaaacg
    26341 attatgggtg gagtttttgt agggttagta attgtattat ctagtatttt agtaatatac
    26401 aatatatttt atatttcaat agttacaaag gttcaagaat ttggaaaact tagagcaatt
    26461 ggtgcaacta agaaacaaat aaaaaatata gtgtttaaag aagggttcat attagccgga
    26521 atagctatac caataggaat aattttaggc tatgtacttg caaatattat aataaaatca
    26581 tttatggata tagatgtaaa atcatcacga ttaccagtta tattatcggt tgtggtaata
    26641 agctttatat ctgttgtttt atcactttta aaaccaatga aagtagcatc aaaagtatcc
    26701 attgttgacg cagtaagata tacaggaaat aaaataagta ataagagtaa gagaaaagga
    26761 tataaaaata taaatctaaa gagattatca catgccaatt tagaaagaaa taaaaaacgt
    26821 acttacatga cattggcatc acttatactt agtggaacta tatttataac agtatcaact
    26881 gccttagaaa gttttgatgc tgagaaaatg gcaagagaac actttcctta tgatatagaa
    26941 gttagactat ctggttatga aatgaacagt gataaaaatc ctaaagataa tttgaatatt
    27001 ttacaaatgg ataatcctct aagtaaagat ttttttaatc aaataaagaa catggaagga
    27061 attaaaagaa tagaatctgc aagaagtgta aaaattggta tggaggacta tgatgttgaa
    27121 tttaagtatg accttttaca aagtataaat gaaaatgatg taaaatcatt aaacaagaat
    27181 ataatagatg gaaaaataaa tcttgaaaga cttcaaactg gagatgaaat agtaattact
    27241 catgttgata cagcaaaaga aatgggagta aaagcaggag ataaaataag attaacttta
    27301 tatgatggag ataaaaagat aaaaaaagaa tttaaagttc aagcaatagc tatgggggtt
    27361 ccaagttttg gtatagggaa agattttata gatagaactt tgaaatacga ttcaactagt
    27421 agcttgggaa tatatacaga agaaggaaag tatcaagaaa tcaaagatag tatcaagaag
    27481 attgctaaaa ataatggatt tttagagact gattttatag atagtagaat tgagtcaaat
    27541 aaggctacta tttcatttat aaagataatg ggatacacgc taacaggcat aataggtgtg
    27601 attggattta tgaatcttgt caacacaatg ataactagca tagttactag aaaaaaagaa
    27661 cttggtatgc ttcaagctat tggtcttact aataaacaat tagtgaaaat gcttaattca
    27721 gaggcaatat attacacttc tggaatgatg ataggaagta ttctatttgg aggtatatta
    27781 gggtatattg ctgttatatt tctaaaaaag actggattat cttatgcaac atattctttg
    27841 ccaatagtac caatcttgtt gatgattgtg tgtattctta tagctcaatt tataacaact
    27901 tatttgatag gacgaagttt taataaagaa tctcttattg atagagtaag atacagtgaa
    27961 taaaatgcaa atatttatgg ttagtttaac tagcaaaaaa catataaaaa aatatagtta
    28021 aagagattca taattaaata ggttaaagca tagtacagag caaacaattt aaacagatgt
    28081 ttgctctgta ttttattatg taatcaataa tatatctaat atactgatat tattcatgtt
    28141 agaaaatgta ccggtacaat tctaaaaaag tataaaattg taccatataa aatgaaaatt
    28201 ttgtataata atcgtataga aaataaatag gagctgatat atatgaataa gaagattaat
    28261 tatgcaccag gaccaactga aactagggaa aatgtaagat tagcaagagc agaaaaaaca
    28321 acaaatccag atatagatat tgattttgtt gaattttata aaaagacatg cgagaagttt
    28381 ggtaatataa ttggaacaaa aaacgatgtt tacatattga gtggtgaagg aatattaggt
    28441 cttgaagcag catgtgcatc acttacagaa aaaggagata gagtacttgt aattgacaat
    28501 ggtatatatg gagaaggttt taaagatttt gtaacaatgt atggtgggga gtatgtgctt
    28561 ttttcaagtg agtatactaa aagtatagat attgatgaat tgaaagcatt tttagatgag
    28621 gatagtaatt ttaaatatgc aacagtagtg cattgtgata ctccaactgg cgtattaaat
    28681 gatgtaagca aaatatgtcc attacttaaa aaatatggaa tattaactgt agttgattct
    28741 gtagcaggta tggtaggaga aagattaagt gtagatgaat ctaaaataga tataatatta
    28801 ggtggttcac aaaaagctat ttctgcacca gcaggactta ctatagtcgg aataagtcaa
    28861 gatgcaaaaa attgtataaa aaatagaaaa actgatgtaa taggttttta ttgtaattta
    28921 aatatatggg aaggctatta tgagaaaaaa tacttccctt atacaatgcc aataagtgac
    28981 ataatggggc ttgataaagc acttgataat atattagaag aaggaataga aaatgtatta
    29041 aatagacatg aaaagatagc ttatagtgtt agaaaatcta ttgaggaata tggtctagaa
    29101 ctatttttag aagaaggata ttctaataca gtcacagcta taaaaattcc tgaaagtata
    29161 ggagctttag aacttactga ttatatgctt aaaaattata atacacttgt tgcaacatca
    29221 ctaaatcaat atatggatac tattttaaga ataggtcata tgggagagaa tgcaaatcta
    29281 aataaaatag aacatgtact aaatgttttg gataaaagtt taagtgcatt aggattcaaa
    29341 ggaaatggaa atttgttaaa tttatttaat aagtattatt tttaatagat agggggatat
    29401 agtatggaag ttacagctaa gcaaaaattt acaacgaaag acatggtgga aacctcacta
    29461 ttaatagcat tagtatttat tgcaactaaa tttataaaca taagattacc aatatctata
    29521 aatggtgggc ttgtgcattt aggaactgca atgttgttca tatcagcaat agtatttgga
    29581 ggtaaaaaag gtgctttatc aggggctatt ggtatgtcac tttttgattt gatatctggg
    29641 tggactttat gggcaccgtt tacctttata gttagaggag ttatgggtta tttacttgga
    29701 aagattgcat gggcaaatgg taataatggg aataattttt taatcaatgt aataggaata
    29761 tgtgtttcat caatttggat gctatttgga tattatgtaa cagaagttat actttatggt
    29821 aattttatag tacctcttac ttcaatacca ggtaatttaa tgcaagtatt gataggtctt
    29881 ataatagcgc ttcctatttc taaggtattg aaaaaatgta taaaataaat ataaagtgaa
    29941 ctaaaattta tgatagcata aattatactg tataatatta ggatggagta cataattaaa
    30001 aaattttagt atagaggtta gttcacaatg ttatcagtta caagatattc aatcaaaaat
    30061 gttcgtttaa agccgtgggt aaagtttatt tggtattttg agacaaaatc aaatatactg
    30121 ttaaataata agttacttcc gacagacagt atagatatta ttctcaatct ttctgatgtt
    30181 atggaatata aaattgaaaa tcaagactat actgctagta atatgcattt taatggaata
    30241 agggataaac atggatttat tattcaacat ggaaatatac gtgttatagg gatttctttt
    30301 tatccatttg gtctctatcc atttttaaag ataccaattt cagagtttaa cagacaaatt
    30361 gttgacctag aagcagtatc acagctattt gcaaaaaaat tggaagagtc attaaatcca
    30421 acacaatcag tagaaaaaat tgttttatat ttagaagaag tattattatc catattagaa
    30481 gaagatttaa tttcaaataa atatgtaaag cttctaaatt catttatata tgttaataga
    30541 tatagcaata taaaagcatt ttgtgatgat acaaatataa atattaaaac attggaaaga
    30601 atctgtttaa aatatacagg gtatacacct aagattttaa agagaattta cagatttaaa
    30661 atggcaagca atcaacttat ttataattat aaagatgatg aactttttga ttttatttat
    30721 gaaaatgagt actatgacca agcacacttt ataaaagaat ttaaaaaatt ttcagggacg
    30781 tcaccaatta aatttattgg tgagaataag acgattaaag aaaatataaa gtacagctat
    30841 ttataattat actgtcgatt ttttacaata cctccaattt attttttgat atgatatagg
    30901 aaaataaatt ggaggtttta catatgaaaa ttattacaga aatagttgag tttaatgtgg
    30961 aagaaaattt atcaaaagaa gagtttatag aaattgtaaa tgacttagaa ttaaaatttc
    31021 attctattca atctggtttt attgatactg agcttttata tgatgaaaag tctcaaaagt
    31081 ggattatgat acaacattgg gaatctatag aagacctcaa gagtgcttct agtaaaatgt
    31141 ttaaagattc ttcaactgag aaatttagaa aagctttaaa gaatcaaact gttaagatga
    31201 atataatacc acaaattaaa agttggaaat tatagaactt aatgtaaaag atagttaaat
    31261 agtatcaaac taaattagcc aaataacatt ttgtatttaa attgttattt ggcttttagt
    31321 gtttatttta atatgagtgt tatatactag aaaaaccaac cttaaatact actatctaaa
    31381 agcactattg ctgtacgaga ggcaataggc attttagaag aaggaggagt tgagctaaag
    31441 tatacatcat aatctcttaa gttttctttt tttgtgtttc ctccaaactc taccatatca
    31501 gtatttaata tcgttttgta gtttgtattt tgaaactttg aaatatcaac tgattgtatt
    31561 agattagggt ggaaattaaa tataaataaa tatttgccct tactgtacac caaaatcttt
    31621 ttatcattat ccatatgtat aagttcatga ctattttgga taaaaatatc actgtatttt
    31681 gtaagttcga gcatagattt atcaaaattt aataattgtt tatactttaa gttgttatct
    31741 tctgaaaggc tccattgtct tctggcatac ttatagctcc aattgttgcc ttctcgtgga
    31801 aaatcaatcc actcaggatg tccaaattca tttcccataa aatttagata tccttcacct
    31861 gctagagaaa aagttattaa tttaattaat ttaataagag atattgctct attgataaca
    31921 tggttatttg agtttatttc catattccag tacatttcct tgtcagctag ccaaaaaata
    31981 attgttttat cgccaactaa tgcttggtca tgggattcac aatatcctat atttttttct
    32041 ccaggtcgtc tagtagtaag ttcataccac atttttccca aatcccagtc ttcatcagat
    32101 aagttagaga tagtttttat ccaaaaatca ggtactccca ttgcgagtct atagtcaaat
    32161 ccaattccgc cgtcttttat agggatacac ataccaggca ttccactcat atcttctgca
    32221 atacttatcg aattaggttt aatttctttt attaattcat ttgcaaattg gagatatgtt
    32281 atagcttcaa tatctgtatt catactaaaa tatttttcat aactatcaaa tgaaactcca
    32341 agaccatggt tatgatataa cattgaagtc acaccatcaa acctaaagcc atcaaaatgg
    32401 tactcattta accaaaattt tatatttgaa agcaaaaaat gtattacttc aggtttacca
    32461 tagttaaaaa gttttgtacc ccaagcagga tgatttccct ttgaacctga gtggaaaaat
    32521 tgatgttcac ttccatcaaa ctcatttatt ccttctaaag tattctttac agcatgagaa
    32581 tgaactaaat ccaataaaac agcaatcccc attgaatgag cagtgtttat gaggtttttt
    32641 aagtcttcag gagtaccaaa cctagatgag attgcataaa agtttgaaac ttggtatcca
    32701 aaagatgcat aatatggatg ttccataata gccataagct gaatggtatt gtatccagct
    32761 ttttttatct taggaagtat tttttcagta aattcattat aagttccaat agattcgctt
    32821 tcagtagaca tacctatatg acattcatag attagtggat aagttatgtt ttttaagtca
    32881 aaatcattgt cagtccagat aaatggagtt tttggttgcc atatttgtcc attaaatcca
    32941 ccaaaatctt tttgaattac tctttttata tataatggta tacggtcaaa tgtttttcca
    33001 tttgcagtaa cttgtacctt tacctctgat ttgtgaggta agctattttg accaggaata
    33061 aatatttccc aattaccaga cccaattttt cttagaggat gagattttct attccaatta
    33121 ttaaagtcac caattagtga caaactatct gcatttggag cccattccct atagaagaat
    33181 ccatcatttg tctgatgaaa tccatagtaa agatgagcat ttgcaaaaga actaaaatct
    33241 ttatagtttt tcaatgtttt tgatttcata ttctcataat gtttcattcg cttttttata
    33301 tcagattcaa atggttgtag gtatacatca atatctaaaa ttttaaatct tttttgactt
    33361 ttcattgtat ttctcctcca taataaatat tacatctaaa aaataatatt tatctcatac
    33421 tatcttctct atataattga tatatattat ataatcctaa taaaaagctt aagccaccaa
    33481 tgattaattt cataatttac ctccctagta tttatactta tatatcgtat tcatcaaaat
    33541 ttaaaagttt gtttaaaact cttttaataa tatcataatc aaagccttta taggaaagat
    33601 gttgagaaat tttttgatat attttctttt tatcttcttt ctttactctt tcataccttt
    33661 tttttgctag atacatagca ttttcaaatt cagtgtcctt gtctaattca gatactgctt
    33721 cattgattgt acttctattg atacctttat tatataaatt ctgcttaatt ctgtttttgc
    33781 cacatttatt taggttaaca tttgtgttaa caattttttg agctaataca ctgtcattta
    33841 cgagattata acttttaaga aaatctatga ccctctcaat tatattttca tcaaattctg
    33901 atgatagttt ttcttttaat tttttttcag attggtcagc atgagataaa atatttaatg
    33961 ctttattttt agcttttata tacatttcat catctaaaat aggttttaat tcttcttcat
    34021 ttatttcatt cccctttttt aaattaaatg tataaacaag ttctttaaat atagcaataa
    34081 aaaatttatc atctacataa atattaactc tatcatcatt tcttttttgt tgttctattt
    34141 ttgtaattat acccataagt cctccttttt tatatgtaat tatattaaca aaagcaacta
    34201 ataaaattta acaaaaaact tatatttgta tgttatattt tatcaaaatt aagacaaaat
    34261 agtaaaaagt tatttttatt aaaaagtaaa gtagaatcat gttaaataat tattttttta
    34321 agtgtttttt agcatataaa tataattata tacccaaaaa aacaaattat aagctttaag
    34381 aaataaaaaa taatggtaca atagtattat aacattaaaa ggagacaaaa tatgagaagt
    34441 gaaaatctag ttaagatggc agagttgaaa aatactgaaa aactagagaa atttaatcgc
    34501 cataaaggca aaatcaaaat aggtatattg ggaggtactt ttgaccctat acactatgct
    34561 catttagcaa cagcagaatt tattagagac aagtatgaca ttgacaaaat aatctttata
    34621 ccatcaggaa atccaccaca taaattatgt atcactacag ataagtatga tagatataat
    34681 atgacacttc tagcaactga aagcaacgaa gattttttag tttcaaaagt agaaatcgaa
    34741 agaaagaaaa ggacttatac aatagataca ttaaaatact taaagaaaaa atacaaaaat
    34801 gcagacattt attttataac aggtgcagat gcaatttgta gtgttgaaga gtggaaagat
    34861 gtaaagaaaa actttgagtt agctactttt atagccgcaa ctagaccagg aattagtttg
    34921 ttaaggtcac aagagactat agaaaaacta actaagaagt ataatgctga cattataaca
    34981 gtttatgttc catcactgga tatatcgtcc acatatataa gggaacagct aaatgaaggc
    35041 aagtcaatac gttacttagt acccgaaaat gtagaaaatt atttatatga aaataaacta
    35101 tatcaatatg gagatgatta aatgaactta gaaaatatta accaaagact aaatcaaatg
    35161 ctaccagtgg ggaggcttag ccattcaaaa aatgttgcaa aatgtgctga aaaattatgt
    35221 gagatttatg gatgtgacaa agaaaaagct tacttagcag gaatgattca tgattgtgca
    35281 aagtatttaa gtgacaagga aatagaggat tatgtaaata agtatgaaat atatttagac
    35341 ccaatagagg atggaaatcg ttccttgtct catagcgtaa ttggggcgta catatgcgag
    35401 tatgaatttg aagtagaaga tgaagatatt ataaatgcaa tcaaatatca tacaacaggg
    35461 agagaggaca tgtctctttt agaaaaaata atttacatag ctgatttaat tgaagaagga
    35521 agaaaattcc cagttgtaga tacattaaga gaattagcat atggagggaa gcttgatgaa
    35581 gcacttttaa cttcttttaa taatacttta atgtttgtca tcaataaaaa agaagagata
    35641 catccaagaa cagttatggc aagaaactat ctaattaaag aaaagttatt ataaaaaagc
    35701 tatataaagg caaacatatt aataaattta aatttgacta tgttacaaaa tcgtattaat
    35761 atgttacaat aactaagtgt attttaatta ggaaagtgag gaatttttat atgacagtgg
    35821 aacaaatgac aaaaatagca tatgatgcca tagaagataa actaggtcaa gatacagtta
    35881 taataaatat aggaaaagtt tctagtttgt gtgattattt tattattact acagcatctt
    35941 ctcagagaca ggtaaaagct attgctgaca atgtggaaga tgaattagca aaactaggat
    36001 tagagccaag aggtaaagaa ggacaaggaa ctcaaacttg ggtactcctt gattatggag
    36061 acataatggt tcatgtattt aatgaggaaa atagagggtt ttataattta gaaaaattat
    36121 ggaaagatgc accttatata gatattgaca cattagcata aacatgataa tatatgttat
    36181 tataattaga aaatttcaaa aattatatta aattgaatag actagagcag taaaagatta
    36241 tattttttca gagagttagt ggaaggtgag aactaatatt atataacttt gaacttacta
    36301 gattaaaatc tattctccat ggctggatta aaagctatct aagagagtct atatatgtag
    36361 taggctaatt agggtggtac cgcgaagatt tatcctcgtc cctaaacgta agtttagtga
    36421 cgaggatttt ttattttcaa agaattattt ggaaattata agtaaataat actaaataag
    36481 gtagttatac acaattaaaa ataggaggca aaatatgagc gtatataatt ttaaagaagt
    36541 agaatcgaaa tggcaaaaaa tctggaaaga taataatcag tataaaatgg atactgcaca
    36601 aactgaaaaa cctaattatt acacattaga aatgttccct tatccatctg gaaaaataca
    36661 catgggacat gttagaaatt attctatagg tgatgttgtc gcaagattca aaaaaatgga
    36721 aggatataat gtacttcatc caatgggatg ggattctttt ggattaccag ctgaaaatgc
    36781 agctataaaa catggaatac atccgcataa atggacaatg gaaaatatag aagaaatgaa
    36841 agagcaatta aatttattag ggattagtta tgattgggat aaagaagttg caacttcaac
    36901 tccagaatac tatagattta cacaagaaat atttttgaag tttttagagc atgggcttgc
    36961 atataagaaa aaatcatatg ttaactggtg tccttcttgt gagactgttc ttgcaaatga
    37021 gcaagttgtt caaggagcat gtgagagatg taaagcaaca gtattaaaga aagatttaga
    37081 acaatggtat tttaagacta ctgagtttgc agaagaatta cttaatgact tagatacatt
    37141 agatggatgg ccagaaaaag ttaaaacaat gcaaaaaaat tggataggta aaagtacagg
    37201 agcagattta gtttttgata tagatggaac tgataaatct atgacagtat ttacaactag
    37261 accagacaca acttatggag ttacttatat ggtacttgct ccagagcatg aattagttaa
    37321 agaattggtt gcagggactg aatatgaagc agatgttgaa gcttttgttc aaaaaatgca
    37381 tactatgaca gaaatagaaa gaacagcagc tgatgtagaa aaagaaggaa tgtttatagg
    37441 tagatatgtt ataaaccctt taaatggcaa gaaagttcct ctatggatag ctaactatgt
    37501 attagttgag tatggaacgg gtgctataat ggcagttcca gcacatgatg aaagagatag
    37561 agattttgct gaaaaatata atttagacat aattgatgta ataacagaag ataacaaaat
    37621 gataaattca gaagaattta atggattaga tgcttcagaa ggatttgaag gaataatcga
    37681 taagttagaa aaagaaggta gaggaaagag aacaattaat tatagactta gagactggtt
    37741 agtttcaaga caaagatact ggggttgtcc tatacctgtg gtttattgtg atgaatgtgg
    37801 aatagttcca gttaaaaagg aagatttacc agttctttta ccaacagatg ttgaatttac
    37861 tggaaaaggg gaatctccac ttacgacttc aaagcaattt atgtcaacaa cttgtcctca
    37921 ctgtggaaaa ccagctagaa gggaagttga tactatggat acatttgttg attcttcttg
    37981 gtatttctta agatatgtgg atagcaataa tgaaaatgaa ccatttagta aggaattagt
    38041 taatagatgg catccagtag accaatatat aggtggagta gaacatgcta taatgcattt
    38101 actttatgca agatggtttg taaaggcatt taagagtatg ggaatggtag acttcaatga
    38161 gccatttaaa aacttactta ctcaaggaat ggtacttatg gatggttcta aaatgagtaa
    38221 gtctaaaggg aatacagtat ctcctatgga tataatagat gagtatggag cagatactgc
    38281 aagacttttc gtattatttg cagcaccacc agaaagagat ttggactggt cagagcaagg
    38341 tgttgatgga tgctttagat tcttaaatag ggtttataga ttggttgacg agttagcaga
    38401 tgtggttaaa aaggatgtag agtttggaga attaaattcc caagataagg atatgagata
    38461 tactatacac tctacactta aaaaagttac tgctgattta agtgaaaaat tcggatttaa
    38521 tactgctata tcagctctta tggaattaat aaatgatatg tataaatata aagagttaga
    38581 taatataaac gaagcagtta taaaagaagg tgtacaaact atagtaacta taattgcacc
    38641 atttgcccct catttaggtg aagaactatg gactatgata ggtaaagaag gaagtgtatt
    38701 tgatatagac tggcctaaat atgacgaaaa agctttagta aaagatgaaa tagaagttgt
    38761 tgtacaagtt aatggaaaag ttagaggaaa attaactgtt aactcaaata tatctaaaga
    38821 tgagatggaa aaggtagctt tagaagatga aaaaataaaa ggattagttg aaggtaaaac
    38881 tatagttaaa gttgtagctg tacctaaaaa attagtaaat atagttgtta aataactcaa
    38941 aaatatgaac aaaaatataa atttataaaa taaatgaggc tgtctcaaaa tagagataag
    39001 ccttttttca ttgttgaaat tataaaaata gatttgattt tatacaaaaa aatgattaaa
    39061 gaactaaaaa gttcattctt atatacccta gttgttgttt taaatattat tataattaat
    39121 gttaaatcga gttaatgtat taagtggtat gtaaatcatt cagtttccaa agtgttgttc
    39181 gtcaaggaac tgctctaagc ttaaacgaaa aaaatataat tattgtattt tgaaattttt
    39241 ttctatataa tgaaaacagg caaaaaaggt gcatatctat gctttatatg aaacacccag
    39301 acaacgaaat tcagattcat ataatagcta ccatgtttct aaaaaggaac ttgtaaaaaa
    39361 gatagcgttt caacttccca gttgattaaa attatcagca ggaaaacaag tatggtcagc
    39421 agtggtattc taatgggtgt tgtcgatata tccaagttgg gattgtctga ggatgttatc
    39481 aaataacttg tgataggtgc agtagatgtg gagattcctg atacggattt attctcaggg
    39541 gatggttgct tcgggagata tggagcgtaa ccagtttatt gatatggtaa ccattttttg
    39601 tctaatttga tgaaagaatc ccaagaatac catacagaag taagacagga attaactggt
    39661 ggtataaact gataaaggca aatttaaaaa ttttgatagg atgattttaa gatttatctt
    39721 ctattttgtt ttttagaaat aattgaaatg ttatgaggag gtatgtacaa atggagactg
    39781 taacaaataa tcaatcaggt gtagaaattc aactcagtct gtttgaagct atcaaagatt
    39841 tattgttaat taatcctatt ttcttgttgg gaattatctt gattggtatt ttcttagttg
    39901 taatattcaa gaaaaatagt gatattccaa aggtcaaaac atctatttta tcactggcaa
    39961 tgtattacta cttatgttta acactatcgc atattgttgg tatacctaca ttcggtgaat
    40021 atataagact gtcacagtta ggggaaacgt tctttaatcc aaacataaat cttattcctt
    40081 ttagtgatgg atttagtttg agttttattt tgaatatttt actttttgtc ccattaggtt
    40141 ttttatgtcc gcttataagc aaaacctttg aacgtttgag aaatacattc ttgattggtt
    40201 ttggattatc tctttttatc gaaactattc agctttttac gttacacaga gcaaccgata
    40261 tagacgactt actcacgaac gtaattggaa caataatagg ctatttctgc ttcaaactga
    40321 ttgctaaatt gcgaattgta aaactacatt cagatgatga ctctatggaa caggattaca
    40381 cagtatgtat tccgattgcc attataatag ttgcccttat attaggattt tttagttaag
    40441 ttccaaatag ctaatttggg aatccagaca atataacaga tactatgtta ccttttacag
    40501 gagcatacta tctgttatat tgtcaacatc ctattttatg gtttgtgtgt agtttcctgt
    40561 aaactgaact aaataattaa atagaagcta ttagaattaa taataacaga tagatattaa
    40621 taagcataaa caaatagtaa aatgtgtaga tatagacgtt tttaagtgaa actattataa
    40681 aataattgac tgataatcaa ctgacaaaac ctattttttt aggtttaact gacatttaac
    40741 tgacagtcag tttttactat ttaaatagaa gttcgcttag attgtcggaa gcttctttgt
    40801 ccatttcttt aagtacatga gaatatctat tcatagttat ttttatatct gtatgaccca
    40861 atctttcaga aacaactttc atattagttc cccctaaaag cagcagagtg gcacttgtat
    40921 atcttaaatc atgtattcta atctttttta aattattttt tttgtaaaat tgtgaaacgt
    40981 ttttcttagt gaagaaggta gaaacggatt taaattggtg tttagacaaa ctaagttatt
    41041 tctgttttcc aggatacctt acaattttaa tttgctttgt cttagttttt caattttcaa
    41101 tttttcaatt aattcttttg gagctgataa agctcttttt gattttggtg tttttggctc
    41161 tttaaaagat attttgccat tagaatagac aagaatttga ttaacattta tcagattgtt
    41221 atctaagtca acatctgacc acctaagccc aatagcttct cacattctta aacctagaag
    41281 taacattaaa agaataggta tttctatatt attaccaagt aacttttcta ttagcaataa
    41341 tacttcttct ctattgtata tttcatttgt agaatatttt tctttagaag gaagttttat
    41401 aaaatcacat gaattttctt gtatttctct taatctatac gcttccctta ttacagaact
    41461 tagaaagcta taccttactt tagcactagt aaaggtagaa ttattaaagt tataatttac
    41521 aaaagcttgt actgaactag gatttacttc tgtgagtttt agattcttaa aaaaggttct
    41581 atgtaatttt taacccaaga ttttgtattt gtaacagtat atggagacca attattttta
    41641 ttcatgtcta tatatttata acatctttct acaaatgtca taccttttgg agcaacaaac
    41701 ttattattat ttatagtaga ctttatttca attaaatgtt tttcagcatc ctttttcttt
    41761 ttatagcttc cataactttt ttgtttcttt ttgccagttt cttattccac atatacatag
    41821 aatttttccc atctttttct tataaaagct gatttgatat tcatatatgt acgctccctt
    41881 tataagttag ttttataaaa gaacagttgg actgctctaa ttatgtcatt atctttttaa
    41941 ttctttacag ttacattata acacgtattt gtatacactt aaattaacag gaagataaat
    42001 atcgttaaag gaggaaaaga aaatttaatg tagaattatg taagatatat agtatttcat
    42061 atataaaagg gggagatatt gtgaaattaa agaaattatt atcactaggg ttagccgtgt
    42121 caatgtttgc tgctggtcct atatcagcta atgcacttga tacaatttat actattcaag
    42181 gaaaagataa atatgaaacg gctgctctta tagctgatga acaaaattat actacagcta
    42241 tattaattaa tgcagacagc acaatggctg atggattaag tgcgagtgga cttgcaggag
    42301 caacaaatgc accaattttt ttaactaaga aaaataacat acctaatgca actcttaaaa
    42361 gagtagagaa agctaaaaag gtatacataa taggtggaga aagttctatt gataaagcaa
    42421 cagaaactct tctaaaagat aaaggaatag aggctaaaag acttcaaggt agtgatagaa
    42481 ttaagacaag ctataatgtt gcaaaagaaa taaactcaat taacaaagta aataaagtta
    42541 tattgactaa tgcttttaaa ggagagcctg atgcaatgag tgctgcacct gtagcagtta
    42601 gagataaagc agctatagta ttaactgatg gaaaaagtgt tccatttaat acaactggtg
    42661 tagaaagtta tgtaataggt ggtacttcat caatgagtga caaactagtt aatgatacta
    42721 attcaactag attaggtgga gtagatagat atgatactaa taaaaaaata gtaaataagt
    42781 tctataatgg cgcaaaagaa ttttatatag ctagtggtac tgatttagtt tatgctttag
    42841 ttgcttcacc aatggctaaa aataatccta ttgtactagt tgatattgga agtaataaag
    42901 atatacttaa aaatgctgca aaagtgactg ggataggaaa tctaagtgat aaaattatgc
    42961 tagagtgtga aaataccatc aataatatta gtcttccgtc ttcaaataaa aatataatat
    43021 gtggattaaa gattgatttt gattcaaagc acataatttg gcaagataaa ggaagttatc
    43081 ttgaatgtta tgatgataac cttgaatatt atactgaaaa atataaagac ccaacagttc
    43141 ctataaatat agaaaataca agtacacaaa atttagatgt aacaaaatta aaagtttatg
    43201 gagaagctaa ttcaacatta tatatggggc aatatgctaa aataaaaatg gattttaata
    43261 gtttcggtgt attaaagcca ggagaaaaat gtactattta tttagatata atttcattag
    43321 atccatcaag aagcatgaca ttaacatttt cttatgtagg tgataaagaa aattataaat
    43381 atgaacaagg attggcaagt agaatattat tttataaccc agatggagaa gatttttttg
    43441 aatataaata actagtgggt atgtaaatta ataagggttt tataagttta atttttttat
    43501 tttcgtttta tattaactat gt
//
LOCUS       DBGJFU010000017        43347 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.017, whole genome shotgun sequence.
ACCESSION   DBGJFU010000017 DBGJFU010000000
VERSION     DBGJFU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.017"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            281..745
                     /locus_tag="ACOIAS_000913"
     CDS             281..745
                     /locus_tag="ACOIAS_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HGZ2700632.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            752..1246
                     /locus_tag="ACOIAS_000914"
     CDS             752..1246
                     /locus_tag="ACOIAS_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HGZ2700633.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1262..2287
                     /locus_tag="ACOIAS_000915"
     CDS             1262..2287
                     /locus_tag="ACOIAS_000915"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HGZ2700634.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2274..4721
                     /locus_tag="ACOIAS_000916"
     CDS             2274..4721
                     /locus_tag="ACOIAS_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGZ2700635.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4901..6274
                     /gene="radA"
                     /locus_tag="ACOIAS_000917"
     CDS             4901..6274
                     /gene="radA"
                     /locus_tag="ACOIAS_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGZ2700636.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6277..7347
                     /gene="disA"
                     /locus_tag="ACOIAS_000918"
     CDS             6277..7347
                     /gene="disA"
                     /locus_tag="ACOIAS_000918"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HGZ2700637.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7436..8530
                     /locus_tag="ACOIAS_000919"
     CDS             7436..8530
                     /locus_tag="ACOIAS_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HGZ2700638.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8659..10836
                     /locus_tag="ACOIAS_000920"
     CDS             8659..10836
                     /locus_tag="ACOIAS_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700639.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KETYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TIYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWYIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(11022..11867)
                     /locus_tag="ACOIAS_000921"
     CDS             complement(11022..11867)
                     /locus_tag="ACOIAS_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700640.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            12084..13250
                     /locus_tag="ACOIAS_000922"
     CDS             12084..13250
                     /locus_tag="ACOIAS_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HGZ2700641.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYRVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYVCGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13272..14918
                     /locus_tag="ACOIAS_000923"
     CDS             13272..14918
                     /locus_tag="ACOIAS_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HGZ2700642.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            15015..16388
                     /locus_tag="ACOIAS_000924"
     CDS             15015..16388
                     /locus_tag="ACOIAS_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2700643.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCDTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            16833..17786
                     /locus_tag="ACOIAS_000925"
     CDS             16833..17786
                     /locus_tag="ACOIAS_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGZ2700644.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17824..18786
                     /locus_tag="ACOIAS_000926"
     CDS             17824..18786
                     /locus_tag="ACOIAS_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HGZ2700645.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18811..19797
                     /locus_tag="ACOIAS_000927"
     CDS             18811..19797
                     /locus_tag="ACOIAS_000927"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HGZ2700646.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19810..20856
                     /locus_tag="ACOIAS_000928"
     CDS             19810..20856
                     /locus_tag="ACOIAS_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HGZ2700647.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            21023..22753
                     /gene="lpdA"
                     /locus_tag="ACOIAS_000929"
     CDS             21023..22753
                     /gene="lpdA"
                     /locus_tag="ACOIAS_000929"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGZ2700648.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            23171..26119
                     /locus_tag="ACOIAS_000930"
     CDS             23171..26119
                     /locus_tag="ACOIAS_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2700649.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            26097..26561
                     /locus_tag="ACOIAS_000931"
     CDS             26097..26561
                     /locus_tag="ACOIAS_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2700650.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            26674..26976
                     /locus_tag="ACOIAS_000932"
     CDS             26674..26976
                     /locus_tag="ACOIAS_000932"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2700651.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            27053..28402
                     /locus_tag="ACOIAS_000933"
     CDS             27053..28402
                     /locus_tag="ACOIAS_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HGZ2700652.1"
                     /translation="MEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGLTVGVG
                     FIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALIIPIGL
                     VVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFASVANMVIVLVMAD
                     LTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQKKFGL
                     FGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLPVSEAA
                     QEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVLPFADL
                     AVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFKMPEGA
                     TMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKLSIEE"
     gene            28437..28910
                     /locus_tag="ACOIAS_000934"
     CDS             28437..28910
                     /locus_tag="ACOIAS_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700653.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            29082..29732
                     /locus_tag="ACOIAS_000935"
     CDS             29082..29732
                     /locus_tag="ACOIAS_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HGZ2700654.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            29906..30838
                     /locus_tag="ACOIAS_000936"
     CDS             29906..30838
                     /locus_tag="ACOIAS_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HGZ2700655.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            31112..31825
                     /gene="ispD"
                     /locus_tag="ACOIAS_000937"
     CDS             31112..31825
                     /gene="ispD"
                     /locus_tag="ACOIAS_000937"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGZ2700656.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            31822..32307
                     /gene="ispF"
                     /locus_tag="ACOIAS_000938"
     CDS             31822..32307
                     /gene="ispF"
                     /locus_tag="ACOIAS_000938"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGZ2700657.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    32402..32610
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            32674..34389
                     /locus_tag="ACOIAS_000939"
     CDS             32674..34389
                     /locus_tag="ACOIAS_000939"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGZ2700658.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            34511..35956
                     /gene="proS"
                     /locus_tag="ACOIAS_000940"
     CDS             34511..35956
                     /gene="proS"
                     /locus_tag="ACOIAS_000940"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGZ2700659.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            36396..37877
                     /gene="gltX"
                     /locus_tag="ACOIAS_000941"
     CDS             36396..37877
                     /gene="gltX"
                     /locus_tag="ACOIAS_000941"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGZ2700660.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            38098..38253
                     /locus_tag="ACOIAS_000942"
     CDS             38098..38253
                     /locus_tag="ACOIAS_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700661.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            38398..39795
                     /gene="cysS"
                     /locus_tag="ACOIAS_000943"
     CDS             38398..39795
                     /gene="cysS"
                     /locus_tag="ACOIAS_000943"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGZ2700662.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            39798..40193
                     /locus_tag="ACOIAS_000944"
     CDS             39798..40193
                     /locus_tag="ACOIAS_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HGZ2700663.1"
                     /translation="MEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRKVNDLHK
                     LAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKHATGFEA
                     LVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            40299..41057
                     /gene="thyX"
                     /locus_tag="ACOIAS_000945"
     CDS             40299..41057
                     /gene="thyX"
                     /locus_tag="ACOIAS_000945"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HGZ2700664.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            41070..41807
                     /gene="rlmB"
                     /locus_tag="ACOIAS_000946"
     CDS             41070..41807
                     /gene="rlmB"
                     /locus_tag="ACOIAS_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGZ2700665.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            41810..42337
                     /locus_tag="ACOIAS_000947"
     CDS             41810..42337
                     /locus_tag="ACOIAS_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HGZ2700666.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            42402..43049
                     /gene="sigH"
                     /locus_tag="ACOIAS_000948"
     CDS             42402..43049
                     /gene="sigH"
                     /locus_tag="ACOIAS_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HGZ2700667.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            43123..>43347
                     /locus_tag="ACOIAS_000949"
     CDS             43123..>43347
                     /locus_tag="ACOIAS_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGZ2700668.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPN"
BASE COUNT        17767 a         4484 c         8123 g        12973 t
ORIGIN      
        1 ttattattat ttttacaaat gtttagtaat aaagatgaga aggtatattt aaaatataaa
       61 gtatttaaag ttctgaaatc taatcatgaa gcaaaaaatt agcatttggt tatcaataaa
      121 tataataaat aaaaactcat aattaatgaa agaataaatt catgtctaaa tttctataaa
      181 aaatttaaat ttatacaatt tagggcttta aaaatttaaa taataggtgt aaaataatag
      241 tcaaagaaag tcaaaaataa aaaggtgtgt ggtgatatta atggcaacta tgacagatat
      301 aattgaaaaa tttattaaag atttgatgga agaagataat agcatacaaa ttcaaagaaa
      361 tgaactagca aacttgttta gttgtgcacc atcacaaata aattatgtct taacaacaag
      421 atttacaata gatagaggat attacattga aagtaaaaaa ggtggcggag gatatgttca
      481 aatagaaaaa atacgaaaaa gtaaagatgg gcatattaga gaactactaa atgaaaaaat
      541 aggaagtcag atatcctata aaaaagcaaa agaattatta gaaggtttaa aagagtcgga
      601 tttgattaac gaaagagaat taaaattaat attatatgca atagatgata aatcattgtg
      661 tatgccaata tatgaattaa aagagaaggt aaggtctaat attttaaaga atataataat
      721 aggattattt agtatagagg agtgataatt tatgctatgt caaaaatgca ataagaataa
      781 agcatctgta tattataata agatagtcaa tggagaaaaa acagagatgt atttatgtag
      841 tgagtgtgca aaagagaata cagaaatgaa ttttaactta gatatgcctt tttcaatgat
      901 ggatatattt tctaatttag gttttcaacc taaaaaagaa cttgaggaaa agctagtttg
      961 tcctaaatgc aatacaacat atagtgagtt taagaataat gggagatttg gatgtagtga
     1021 atgttataat gcatttagta gtcaagtaaa tccaatgctt caaaatatac atgggcatat
     1081 agaacataca ggaaaagcac ctaaaaaatc tttttataag ataagtgtag aaaatgagat
     1141 taaagaacta aaagaagatt tagatagagc tataaaaaat gaagagtatg aattagcagc
     1201 tcaatttaga gataaaatta aatatttaaa aggttctatt gactaagatt ggagtgatat
     1261 tatgaaagag aacatagtca tgaaatcaag agttaggcta gctagaaatt taaataacta
     1321 tccatttcca aataaattag ataaagaatg tgccatggaa ataatagaaa aagtaaaaaa
     1381 tgcgtttatt aattctaact tagaacaaaa agaagaattt gatttctata aaattgaaga
     1441 tttagaccaa tctaaaaaaa tgcttatggt tgaagaacat ataataagcc cagatttagc
     1501 agaaaatgat aaaagtgcag ttatagtaaa aaaagataaa actataagta taatgataaa
     1561 tgaagaagac catataagga tacaaactat atgtgatgat ttaaacttag aatatgcata
     1621 tagtgtagca aatgaaatag atgatttgtt agaatcaagt ttagaatatg cctttaacac
     1681 aaagctaggt tatctaacat cttgtccaac taatacagga acaggaatga gagcttctgt
     1741 aatgatgcat ttaccagcat taagtcaact aggctatatg gatgaacttt ataaaatttc
     1801 atcacaaata ggtatagcaa ttagagggat atatggagaa cgaacagaag cattgggaaa
     1861 tatatatcaa atatctaatc aacttacact tggaagaaca gagtctaata ttatagaaaa
     1921 tgtaagtgga cttacaaaag atgcaatttc aaaagaaata aaagctagag aaatattgca
     1981 aaaaaaatta ggtataaaac tagaagataa aatatttaga tcgataggga cacttgaaaa
     2041 ttcaagagta atgagtagcg cagaggcaat gagccatcta tcaaatataa aaatgggaat
     2101 agaaatgaac tatatagata aactagattt aagggctata gaacaactta tgataggtat
     2161 acaaccagcg catcagtcaa taatgtataa gagtgatgat gttgagaata gagatataaa
     2221 tagagcaaca tatattagag aaactttgga aaaattaaga ggagggatga attgtgaact
     2281 tcaatagatt tacacaaaga gctaaaaagg caattgattt agcgtttgaa tctgctaaaa
     2341 gtctaggaca caatattgtc ggaagtgaac atatactttt aggtctttta agagaagaag
     2401 aaggtatagc tgctaaagtt ctaagtaaag ttggatttac agaagcttat ttagaaggca
     2461 aaatagttga tatggaaggt aagggagaag aaatctcaga agatatagta ttaagtccaa
     2521 gaagtaaaca aatacttgaa ttatcaggaa tgtttgcaaa taaattaaaa acaaattata
     2581 ttgggactga gcatatatta ttagcaatta ttcaagaagg tgaaggaata gctaataaga
     2641 ttttaaatta tgctggagta aatgatagga ctttagccca attaacaatt gatatgatgg
     2701 gtattagtga taaaaatcaa tacaaagcag aaaatagtta cacgggcaac cagaatcaag
     2761 cagaatctaa agtacttgat aagtatggaa gaaatcttac actatatgca aaacagaata
     2821 aaatagaccc tgtaataggt agagaaaaag aaatacaaag agtaatacaa atattaagta
     2881 gaagaactaa aaacaatcca gtgttgatag gagacccagg tgtagggaaa acagccatag
     2941 ctgaaggtct ggctacaaat atagccttag gaaatgttcc agaaacacta aaaaacaaaa
     3001 ctttatactc tttagagatg ggttcattat tggctggggc taagtataga ggagaatttg
     3061 aagaaagaat taaagaagtt gtagatgaag ttgttaaaaa tggaaacata atcttattta
     3121 tagatgagat gcacacaata ataggggctg ggtctactgg agaaggctct atagatgctt
     3181 caaatatatt aaagccagcc ttagcaagag gagaaataca agttataggt gcaactacaa
     3241 ttgatgaata tagaaagcat gttgaaaaag attctgctct agaaagaaga ttccaaccag
     3301 ttatggtaga tgaaccaact aaagaggact caataaaaat attagaagga ttaagagata
     3361 aatatgaagc tcatcataaa gttaaaataa ctgatgatgc tataaaggca gctgtagaat
     3421 tatcaactag atatatatca gacagatatc tgccagataa agctatagac ttgattgatg
     3481 aagctgcatc aaaagtaaga ttaaaagaaa atacacctcc ttcagaaata aaaaaattag
     3541 aactagaaat agaaaatata gataaagaaa aagaagaagc agtaagatgt caagactttg
     3601 agaaagcagc caaaataaga gatgaacaag gtctacttaa aaaacaatta gaagatgtta
     3661 gagaaagatg gaacaagtca tctaaacatt cagatttagt tgatggagaa gttattgctg
     3721 aagtagtggg cttatggaca ggaataccag ttaataaaat tcttgaagaa gaggctgata
     3781 gacttttgag acttgaggaa atactgcaca atagagttat aggtcaagaa caagcagtaa
     3841 aatctatttc aaaggcaatc agaaggtcaa gagcaggtct taaagaccct aatagaccaa
     3901 ttggttcatt tttattttta ggtccaacag gagtaggtaa aacagaatta tctaaggcat
     3961 tagcagaagt gcaatttgga gatgaaaatc aaataattag aatagatatg tctgaatata
     4021 tggaaaaaca tgctgtgtca agaatgatag gttcacctcc aggttatgta ggtcatgatg
     4081 aaggtggtca attaactgaa aaagtaagaa gaaatccata ttcagttata ttatttgatg
     4141 agatagaaaa agctcatcca gatgtattta atattctgtt acaaattcta gatgatggta
     4201 gactaactga ttcaaaagga agaactgtag attttaagaa tactattgta ataatgacat
     4261 caaatgttgg agcatctaca attggtagac aaaaaacttt aggatttagt atagctaaag
     4321 gagacgaaga agaaaaatct caatatgaaa aaatgaaaga aaatataatg ggtgaattaa
     4381 aacaaagatt cagaccagag tttttaaaca gaatagatga tataattgtt ttccactcac
     4441 taaatgaaaa tcatatatca aaaatagtac ttttaatggc agcgaaacta caagagagat
     4501 taaaagaaat ggatataaaa ttagaaatga gtgatgaggc tgttaaatta atttctaaat
     4561 ctggatttga cttagaatat ggagcaagac ctcttaaaag agctttacaa aaagagttgg
     4621 aagatgagct atctgaagca atcttaaaag gtaatgtgaa aaagggaagt aatgtagtag
     4681 caaaagtcaa agatgaaaaa atagtttttg aaactaaata atttaataga gttgcatttt
     4741 aaaatattgc aaatataagt tttataatct aaacaaaatt ttaagggcaa tgaatctata
     4801 ataattcatt gtccttaatt attttattta atctttacat attgtctttc agatatgcta
     4861 aaattatagt atagaataaa aaattaagga tgataaaagt atggcaaaaa taaaaacaaa
     4921 atatgtatgt caatcttgcg gatatgaaac agctaaatgg cttggtaaat gtccagaatg
     4981 tacaaaatgg aatacatttg tagaagaaat agaccaaaaa agcaccaaaa aagaggtctt
     5041 tataatagat aaatcttcat ctaaaccagt cagtataaac tcaatagaaa gtaaagaaga
     5101 agagcggttt acaacagata taaatgagtt agatagagtg cttggaggag gtatagttaa
     5161 aggctcttta gtgcttgttg gtggtgaccc tggtataggt aaatcaacac ttttaatcca
     5221 agtatctagt aatgttgcca atttaggaaa aactgtttta tatataactg gagaagaatc
     5281 agaatctcaa ataaaaatga gagctaaaag attaggtatt aactcagaaa atttatatat
     5341 atttgctgaa aataatttaa gtataattga atcttattta gaaagtgtaa atccagaatt
     5401 aataattcta gactcaattc aaactgtctt cagtccagaa atatcctcgg caccaggaac
     5461 tgtcagccaa attaaagaag gtacttcaaa attcatgaaa atttctaaaa aaatgggaat
     5521 ttcaacgttc atagttgggc atgttacaaa agaaggttca ctagcaggac caaaattatt
     5581 agagcatatg gttgatactg ttctatattt tgaaggagaa agatataata cttatagatt
     5641 agtaagagct gttaaaaatc gatttggttc aactaatgaa cttggagtat ttgaaatgag
     5701 ggatttagga ttagtagaac ttgataatcc atctaaaatt ttaatctctg aaaaaccaaa
     5761 agatgtagca ggttcagtaa taatatctac tgtagaaggt acgagaccaa tgttacttga
     5821 attgcaagca ttagtttctc ctacaagttt tggtatacca aaaagaacat ctacaggagt
     5881 cgattacaat agagtaggta tgttattagc agtattagaa aaacgtgttg gtttgcaaat
     5941 acaaaatcaa gatgtctata taaacatagt tggaggaata aagataaatg aaccctcaat
     6001 tgatttaggg atagccattt ctgtagcatc tagttttaga aatataccga tagatgaaga
     6061 tatagctgtt acaggagaag tgggacttac aggagaagtt agagcagtaa gctttataga
     6121 aaagagaata gctgagtgta aaaaacttgg atttaaaaag atagtagttc ctagaagtaa
     6181 ttatgatgtt gtaaaagaga ccaaaggcat agaaatatgg ccagtagata acttaagaca
     6241 agctataaat atagtacttg ggaggaatca ataaatatgg agaattttct agataataaa
     6301 aatatgctat atgcattaaa aatgatatct cctggaactc cacttagatt aggtctaaac
     6361 aatgtactaa gagctaagac tggtggatta attgtaattg caacaaacga agatgtaatg
     6421 aaaatagtag atggaggatt tgctataaat gcagaatatt caccatcata tctatatgaa
     6481 ttagctaaaa tggatggagc tatagtttta agtggtgatg taaagaaaat attatttgct
     6541 aatgcacaac ttatacctga ctattttata gaaacatcag agacaggaac aagacataga
     6601 acagcagaaa gagtagcaaa acaaactggt gctatagtca taggaatttc acaaagaaga
     6661 aatgttataa cagtttatag aggaaatgag aagtatgtag tcgaagatat atctaagata
     6721 tttactaagg caaatcaggc tatacaaact ctggaaaaat ataagacagt attggaccaa
     6781 gctgtaacaa atttaaatgc cttagagttt aatgatttgg taactattta tgatgttgca
     6841 ttagtcatgc aaaagatgga aatggtaatg agagttacaa gtataattga aaaatatgtg
     6901 atagaattgg gtgatgaagg aactttagta agtatgcaat tagaagaatt aatgggtaca
     6961 accagaatag accagaaatt aatattcaaa gattataata aagaaaacac agaaataaaa
     7021 gaacttatga aaaaggtcaa aaatttaaat tcagaagaac taatagaatt ggtcaatatg
     7081 gcaaaactat tagggtatag tggtttttca gaaagtatgg atatgcctat aaaaacaaga
     7141 ggttatagga ttcttagcaa aatacataga ctaccaacag caataataga aaacttagta
     7201 aattattttg aaaactttca acaaatttta gatgcatcta ttgaagaatt agatgaggtt
     7261 gaaggaatag gtgaaataag agcaacatat ataaaaaatg gactcataaa aatgaaacaa
     7321 ttagtcttat tagatagaca catatgatga attttaaatg aattttacta cttttttctt
     7381 gaatataata tacactttgg tctataatag ataagtataa ctagggggtg aagcattgat
     7441 aaggaaagta acaagaatat tgtttatttt acttggattt acaataggta taacaaccta
     7501 tttaacatta atgaaagatt ttgaaatatt aacatttgga aaagaaacat atggttatat
     7561 cgcagctgta gtagcaggta taataatagc cattttagga tatcttatag aaccatgggt
     7621 tgtaaataaa gtaaaagaaa ttgctaaaat agtggataaa gaattgtcaa aatatcctca
     7681 gactgatata ttgttagggt ctatgggtct tatagtagga tttgttattg cgtatttatt
     7741 aagtggatta gtaaatagaa taccaatagt aggtggaata ctatccttac tattatattt
     7801 attcctagga tatcttggaa tgaaagttgc gcttaaaagt aaaaatgatt tatttaatat
     7861 tggaaaacta ggaagattag caaatcctat taaggataaa gataaggata aagaaaataa
     7921 aaaagaagta aaagccattc ctccaaaagt attagataca agtgtaataa ttgatggaag
     7981 aatagctgat atttgtaaga ctgggtttat agaaggaaaa cttattattc ctgcttttgt
     8041 tttggaggaa ctaagacata ttgcagattc ttcagatgat ttaaaaagag taagaggtag
     8101 aagaggtctg gatatattaa atataattca gaaagaatta aatatagaag ttgaaataag
     8161 tgagagagac tttgatgata tagcagaagt tgatagtaaa ttattaaagc ttgctcaggt
     8221 tttaaatgga aaagttgtta caaatgatta taacttaaat aaagttgctc aattccaagg
     8281 tgttgaagta ttaaatataa atgagttagc taatgctata aaaccagttg ctataccagg
     8341 tgaagatatg gttgttcaag tggttaaaga aggaaaagag gctcaacaag gtgttgcata
     8401 tctagatgat ggaactatga tagttgttga tggaggaaga aaatatatga atgagactat
     8461 aaaagtactc gttacttctg ttcttcaaac tcctgcaggt agaatgatat ttgcaaaacc
     8521 taaaaattaa tatgttcaaa gaaacctata atttgggctg tttattttaa atagatggtc
     8581 caaatttatt tatttataac gataatgttt aatgtattct taaagatggg gatttaaatc
     8641 agaaagcagg gagaatttat gagaaaaaag attatatctt tcttagcaac atttatcata
     8701 atattaacat cagcaagtca gtatagtttt gcagatgata tatcaacacg tggaaaagta
     8761 atatttatag atatgaatag aactagtatg agtaatatgt taagaataaa atcattaaga
     8821 gaagaactag acaatagagg gtatattggc ttgatgaata taagaggcga taaaggaagt
     8881 gatgatagaa ggtcatatgc tagtatgggt gctggtggta gagctaatgt ggctaatgaa
     8941 gaagatatta attttgaaag ctcgagtaag gatagaaata tagtttttga atcagctaca
     9001 ggaaaaagtg caaaaggtat aaataattta actataaata agtctataaa tgaaaattta
     9061 aactttggag agtatggttc tgtattaggg tcattaggac aaagtttatc tgataatgga
     9121 ttaaaagcat ctgtattggg taattcagat ataattgaaa atggtcaact tataaaaaat
     9181 agaaacttat gtctaactgc aatggatgaa tatggaagaa tacctaatgg aaatgtagat
     9241 actataaata aaaaagactt atctatgcct tatggaatat caactgatta tgataaattg
     9301 attgtagaga ctaaagaaac ttacaaaaat aatgatgtga tttttgtgga gcttggagac
     9361 acatatagat tagatttata taaaccaaat ttaaatgaaa aaacatatga gagtatgaaa
     9421 gataacatag aaaaaaatat agatatatat ttaaaaaata tattttctat ggttgaagaa
     9481 aatgacacta tctacatagc aagtgcattt ccaagtgatt tagattataa aaataaaaga
     9541 agactttctc caataataaa attaaatgga gaagggaaag gtatattatc atcttcaact
     9601 actagaagag aaggtattgt aacaaattta gatgttgggg ttgaaatatt agacaacttt
     9661 aatataaaaa atcaaaatat ggttggaaga aaatatgaac ttattaacag agatgataat
     9721 aaagaatttt tgatggatga atatcaaaag atagtatcta tttcaagcat aaggtctaca
     9781 atactgaatg gttttgtaag tatagttttc ttgtcatgga tagttgctat gattgcaatt
     9841 ttatttagaa agcatatatc aaaaaattat aaagaaacaa cattttttat attaaaggaa
     9901 cttttaaaaa taggaatagt aatgccactg tcatttatga taacaccaat aatgaatttc
     9961 aaaacaccat tagcaatatc tttaggaata attataataa cacttactat atatcttata
    10021 agtaaagttt taataaagaa cgatttaaaa aacatgctgt tttttactgg attaaccata
    10081 gtaattatgg ttattgatgc tgggtttggc tcatatttaa tgaaaagcaa tgttatgagt
    10141 tatgattgta taattggagc tagatattat ggagtaggaa atgagtatca aggtgtggca
    10201 atcggtagtg caatatttac atttgcaatt ttacttacat ataaaaatat accaaagtgg
    10261 tatataatag ttttttcttt ggttatctta attacaagtg catctccaat tatgggagca
    10321 aatgttggaa gtgctatttc tgaatgtgtt gcatttttac tatttatact attgatatat
    10381 aatgtaaaga tagatttcaa aaaaatcgta ttattaggta tagcagtagt atttgtatta
    10441 ggagtttttg tagcaataga tatgattttg ggaagtaatt cacatttagg tatgtttgta
    10501 aaagaaattt actttaatgg accaggagaa ataatacaaa catttagtag aaagatagag
    10561 atgaatttaa agctagctca aaccagtgca tgggtaaata tactgttaac tggaataggt
    10621 gtaattttag ttcttatgat taatcagatt agatacttta agcaactgat ggatgagtat
    10681 ccaattgtat ttaaaggatt tatagcatca atagcaggat gtttagtaac tcttttagtg
    10741 aatgactctg ggattgtgtc atcagctaca gcctttattt atgtaattgt acctatgata
    10801 acattaagta taaatcttac agcattaaaa gaataagtac tgtatgaata atatgtaaat
    10861 attaatacat taatttaact tctttattta caaaataaag aagaaagttt ataaaaaata
    10921 aattataata aaaaagagtt tatctcaaaa ttgattattc taatcaaatt ttaagataaa
    10981 ctttttttat tataaagata aataacatat aacctcatat tttaggtaga tttttttctt
    11041 atatgtttag gagtgcattt aaatttttcc ttaaaaattc tgtaaaataa ttttgtatta
    11101 gtaaatccat gagcttctaa tatactgcat aaggtatcat ttgtgttaat taaatcttta
    11161 taaatatgag aaatacgcac atcatttaaa tactctaagt aagtttgtcc catatactgt
    11221 ttaaattttc tacaaaaata ttctggttgt aagtgtgcta ccttagcgat tttgttaata
    11281 gaaataggca ttttgtaatt aatctttgta tattctaaaa ctggttcaaa ttttgacaaa
    11341 tcaagtactg tttttttcat atttttatta cctacagata ttttaaagtt tgtatataat
    11401 tcaaataaaa gttcaaataa taagcttgta aagagcagat tagctgcagg aggagaacta
    11461 agtttaattt ctctcatgtt ttctaatata agtttaactt tacttgaatt ttttttatag
    11521 ttattgtcat tggcattaac cttaaagtca aaatagcaat ttttaaaatc agggatatat
    11581 ttgtttagaa aagacaatgg tacttgcaat aatatagagg tattttgata aatacatcta
    11641 gttgagtgaa taattccaga attaatgaac atacactcat tagcttttaa attataagtg
    11701 taaccctcca tgttaatttg taaatcacca gaagttatat aaattatttc taatgagtta
    11761 tgccaatgat ttgctacata actaccacta tcatcaaata gtttgaaatt aacatctaaa
    11821 ttatcaatag gttcaattat ttcatgtgaa aaattatttc tgttcataat aatatcctcc
    11881 ttataatttt aaattataaa tagtaaattt ataatgttca atattaaaaa gataatatag
    11941 actaataaat tagataataa acatacttaa gaaaacggat aaaatcaaat ataataactg
    12001 tatattaaaa taacttaaaa aaattatttt attataagtg caaaatatac ctatttaaat
    12061 aagataagag gggaggattt tatgtgtata taaaaggtgt aaatttagga ggttggttag
    12121 ttttagaaaa atggatgacc tcaagtttat ttgaaggcac agaagctgaa gatgaatact
    12181 atttaccaag acagctttca agagaggcat atgaatctag aattaagact catcgtagtg
    12241 aatacatcac agagagagat tttgcaacga ttaaatctat gggatttaat tcagttcgta
    12301 taccagtacc atactttatt tttggagatt gtgaaccatt cataggatgt gttaaagaac
    12361 ttgataaagc atttgcttgg gcagataagt atgggcttag tatattgata gacttacata
    12421 cagttccagg aagccaaaat ggatttgata atggaggaat atcaggtata tgtagctggt
    12481 cacaaaatcc agaatatgta gcgtttacat taaatgtatt agaaagactt gcaaaacgtt
    12541 atgggatgag gcatgaattg tatggaatcc aaattttaaa tgagcctata acagaaagaa
    12601 tgtggaacat aatgaatgtt ccaaatcggt ttaaggctgt agataaggag atggcaaggg
    12661 ggagtaaacc taattcatta gagtttttac gtgattttta tataaaagca tatagggtaa
    12721 tgcgtcctta tatgagagag gaaaacgtta tagtgtttca tgatgcattt gaattaaaag
    12781 catggaaaga ctttatgaga gaggaagaat ttaaaaatgt tgttctagat acacaccaat
    12841 atttgatgtt agctgaagca gatggatgtg aacaaagtat tgatagctat ctcaaatata
    12901 ttagagaaaa ttatgctaaa gatattttac aaatgcaaaa atattttcct gttatatgtg
    12961 gggagtggag cttatttaat tcatatgttt gtggtataga tacaaatgga ggtcaatcac
    13021 ctctaaatgg gattgaatca aatatagata aattatcaaa agacgataaa agagaattat
    13081 ataggaagat agcaaaagca cagcttgatg catggagaaa tggaagtggt cactactatt
    13141 ggaattataa gttattacta gatacagtta atgaagaagg ttggatagga tgggatagtt
    13201 gggacctagg caaatgtgta gctcaagaat ggtatcctat agaatattaa actaaaattg
    13261 gaggaaaaga tatgattaaa aaccctatac ttccaggttt taatccagat ccttgtattt
    13321 gtagaaaggg agatgattat tatcttgtag tttcatcttt tgaatggttt ccaggtatac
    13381 ctgtttatca ctctaaagac ttaaaaaatt gggagttata tactcatatt ttgacagatg
    13441 aaacaaaaat agatttgaag aagcttcctt catctaaagg aatttgggca ccctgtttga
    13501 cttattgtga agaagaagat ttattttaca tagtttatgg aattatgaac tctatgaatg
    13561 caagatattt tgatgttgat aattatttaa ttacatcaaa agatataaag ggagagtgga
    13621 gtgaaccagt atatctacac tcatcaggtt ttgatgcgtc tatttttcat gatgatgatg
    13681 gtaaaaagtg gattgcatca ttagactggg agactagaga aggatatgaa aaacctggtg
    13741 ttatatgttt agtggaatac tgtacaaaaa agaaagaaat agtaggatat ccaaagagaa
    13801 tatggtcagg gggaactgat agaggttgta ttgaagcacc acatataaca aagcgtggag
    13861 attactatta tataatgtgt gcagaaggtg gaactggata tggtcatagt gtaactatgg
    13921 gaagggctaa aaatatatgg ggaccttatg aaaaagattc aatgaatcca attgtaactt
    13981 caattccagg cgatttttat gaaagacatg accctgacca tctaaagcct aaatactata
    14041 atccagaatc aaaacttcaa aaatctggtc atggaagcta tatagaaaca acatcaggtg
    14101 aagtatattt agttcatcta acatctagac catttgtacc agagttaagg tgtactttag
    14161 gtagagaaac agctattcaa aaaatgaaat ggacaaagga taattggctt cgaatggaag
    14221 atgaaagtaa tttggcaaaa gaatacgtat cagaaagtaa actagaagaa catttagttt
    14281 catctatacc aagttttgat gactttgatt caaatgaatt aggtttacag tactatgctc
    14341 ctaggatttc accattatca tttgcagatg ttaagtctag accaggatat gtaaggatta
    14401 gaggacaaga atcaagaaca tcattaaata aagtaagcat attggcaaga aaattaactt
    14461 cagtatatgc aagaataacc actaagatgg agttttaccc agaggtacat cagcatagtg
    14521 cgggtttgat aatgtattat gataatatga attatataaa tttaagaaag tattatagtg
    14581 aaacattggg tcaaagtgca ctatcaataa tacatcttga aaatggagag aaaacggaat
    14641 ttttaaatac taggatacca ataaaagata taccaatata tcttcgtctg tatatacaag
    14701 gtcgtaagtc atattttgaa tggagttatg atgaaaaaaa ctaccagaga ataggaaagg
    14761 tatttgatac aactaagttt tcagatgagt actgtaaata tggagaattt acaggtacgt
    14821 ttataggatt aacttgtgca gatcgtgtaa agcataaaca ctatgctgat tttgattttt
    14881 ttgaatatat agtagatgaa tcaaaagatg tcgattaata aaatcattac taaaattttc
    14941 agagcaaagg taaatcttat gctctgaaat aagaaacatt agaaggcaaa tcttacaaaa
    15001 aggagtatgg tattatgaat acagaagtta atgttaaagc aactacgatt agatatgaaa
    15061 agatttcatt ttttgaaaga atttcttatg gttgtgggga tttggggtgc aatatcatat
    15121 actcagctat gtcagcattt ttgctgtttt attatactaa ttatgcagat gttagtgcag
    15181 cagctgtagg tagtattatg cttgtatcta ggatactgga tgggtttagt gacttaacaa
    15241 tgggaattat agtggataga actaaatcca aatacggtaa ggctaggcct tggattttga
    15301 gaatggcgat tccttttgct atagcagcag tattattatt ttctgttcca tcaaatcttg
    15361 gtatcacttc taaattaatt tatattttta ttacatataa cttagtttct actgttattt
    15421 atacagcaat caatgtaccc tatgcaactc taaattcact tataactcaa aaccagtatg
    15481 aaagaggagt attaagtata tttagaatga tattagccac ttgtgatacc ttactaataa
    15541 atggacttac actacctcta gttgaatact ttggaaataa tttatcagca tggactaaaa
    15601 cattctttgt atttggaata gcatctataa tggtattttt tataacattt actggaacca
    15661 aggaaagagt aaaagcagtt aagcaagata ggaatgaagt aataccattt aaaataggta
    15721 ttaaatcttt atttagaaat aagtactgga tacaaataac attatgttta gtttgcatat
    15781 ttattgtatt tgcgataaat ggtggttcat ccgtatatta tgcaaagttt attttgggag
    15841 atgaaaaatt atttggtcct ataaatatgg tttcaaatat atcccaaatt atagctatgt
    15901 ttatggttgc tccttttata aaaaaatttg gaaaaagaaa tgtattaata gtaggttcaa
    15961 taatattaat atcgtcaaat gttatgttta taatagttgg acaaaatcat ataggagtaa
    16021 tatttgcaag tgctataaag ggaataggaa gtgctggaat agcatcaaca atgtttgcta
    16081 ttgtttcaga tactattgaa tatggagaat ggaagactgg atatagaact gaggggctta
    16141 taaatagtgc atctagtttt gggtttaaag tgggaaatgg attaggttca gcaatattag
    16201 gtgcagtatt atcaattggt ggttatgtag gaacatcagc tactcaaagt gatttagcta
    16261 tagtatcaat aaaagtatgt tttatatacc tacctatatt tataacaata ttacaaatta
    16321 tcataatgtc tttttataag cttgataaag aatatagtac tatacttaat gaattaaata
    16381 ctaaataatg aaaaagtttc ttataaagtc ttaagtttaa ttaaggtcta ttgtaaatgt
    16441 tccctataaa aactattata tatttatgat tattgaagaa tttttagaga agcgaataat
    16501 aaaaatataa gaagacttta aatgatgaaa tggttggttt tttagaaatg atttaataag
    16561 ttattgttat ttaaagcatt ataagtggac aattaatttt taatttatgg agatgatatt
    16621 taacaattag aagtaatata taatatgcac taagaagcaa tttttcaact atttatgaag
    16681 gtggtggtac aagaaaagta tcaccttttt tttgttatac aaaaaaacag ttgacttttt
    16741 atatgaaccc cttctataat gttactgaaa ttaagtaaaa tgattgaaca aatgtaaaga
    16801 taattgaaaa gcttgatttt ggaggaaagc aaatgaaaaa aattggagac ttaagattaa
    16861 tgatgaagtg ttgttcactt tattacgaag ataatttgaa tcaacaggaa atagcaaatc
    16921 aattgggaat atcaagacca acaatatcaa gaatattaaa agaagctttt gaacaaggaa
    16981 tagtaaaaat acagatagtt gatgttttga aaaatgatta ccaaaaaata gagagaagtt
    17041 tggagagaaa atataaatta aaagaagtaa tagtagtaga tgataaacag gatgcactaa
    17101 cacaaaaaca agaactagca agagctgtat ctgaatattt aactagagta gtaaaggaaa
    17161 atgatataat aggtgtttca ataggaacaa cactaaaaga aataccaaga tatgtggaaa
    17221 aaagtaactg taaaaatgtt acatttatac ctctattagg agggattgga gacaatgaaa
    17281 tagatattca tgcaaatcaa atagccgtaa gtttagcgag ggcttttgga ggtgatttta
    17341 aattattaca tgctccagct gttatgtctg acttgtctac aaaagagaaa ctttgcaaag
    17401 acgaaaaaat taaagaagta ctagatttaa tagataaaac aactatagca attgtgggta
    17461 taggaaatcc tatgagttta aactcaacaa taatggcttc tggatacatg aatgaagcag
    17521 atatagaaga tttaaaaaaa tacaactcaa taggtgcaat ttgtttacaa gcctttgata
    17581 aagaaggaaa aacttctata cttgaattta atcaaagagt attaggagtg aaactggaag
    17641 atttaaaaaa gataaaaaga acaataggtg tcgcttcagg agatgaaaaa atagaggcaa
    17701 taaaggcatc attaaaagcg aaatttataa atagtttagc aattaatcac agtttagcac
    17761 ttaaattatt agaagattgt gactaataga aagatagata aaagttaaca ggagggaatg
    17821 tatatgtcaa tatcaaaaga aacattgctt gaaatgtata agagaatgaa tcaggctaga
    17881 aaatttgaag agaaagttag ttggtttttt gcaagaggta tggttcatgg aacaactcac
    17941 ttatctgtag gtcaagaagc gtcaagtgtg gcggctgtaa tggctttaga aaaaggagat
    18001 ttagtatcat taactcatag aggacatagt caatttatag gaatgggaat agacctaaat
    18061 aaaatgatgg ctgaacttat gggtaaagaa actggatttt gtaagggaaa aggaggctca
    18121 atgcatatag cagatattga gtcaggtaac ttaggagcaa atggagttgt aggtggaggt
    18181 cttacaatag ctccaggagc tgcacttaca caacagtata aaaaaacagg caaaatagtt
    18241 ttatgtagtt ttggtgatgg agcatcaaat gaaggtactt ttcatgaagg aataaattta
    18301 tcttcaatat ggaaattgcc aataatattt tactgtgaga ataatttata cggtatgtca
    18361 acaagcataa aacgtcatat gaatatagaa agtattgcca ctagagcagc ttcttatgga
    18421 atagaaggaa tatcaataga tggatataac ccaatagaag tatacgaaac agtacaaaaa
    18481 gctgctgaga agtgtagaag aggagaagga cctgtattaa ttgaaagtag aacttataga
    18541 tggttaggtc attcaaaatc agatgcaaat gtatatagaa caaaagaaga aatagaatca
    18601 tggaaagcaa aagaccctat tgaattttta aaaaattatc ttatagaaaa taatctatct
    18661 aatgaagatg aattagataa gattcaagaa tttgcaaagc aatcaataga agatgcagta
    18721 gagtttgctc aaaatagccc aaatccaaaa atagagtctt tattagagga tgtatatgca
    18781 gactaagaat ggtaaaggag gaaaataaaa atgagtacaa gggaattaac atatgcacaa
    18841 gcaataaagg aagcaatgtc agaagaaatg agaagagatg aaaatgtaat atttatggga
    18901 gaagatatag gaatatatgg aggagcattt ggtgtatcag ttggtatgat agatgaattt
    18961 ggaccagaaa gagtaaggga tactcctata tcagaagcag ctatagcagg agcggcagca
    19021 ggtgcagcag caactggctt aagacctatt atggaagtaa tgtttatgga ctttgtaaca
    19081 atttctatgg atgcaattgt aaatcaagcg gcaaaaatga gatatatgtt tggaggaaaa
    19141 gcacaagttc caatggttgt tagatgccca ggaggttctg gaactggttc agcagagcaa
    19201 cattcacaaa gtttagaagc ttggttttgt catgtaccag gagtaaaagt agttgcacct
    19261 tctacaccag cagatgctaa gggattgttg aaagcagcta taagagataa taatccagtc
    19321 atatttgttg agaataaatt gttatacaga aaaaaaggtt ttgtgccaga agatgattat
    19381 gtaattgaaa ttggaaaggc agatattaaa agagagggta ctgatgttac agtaattact
    19441 tatggaagaa tgttacaaag tgttgaagaa gcagcagaga ctctatcaaa agaaaatata
    19501 aatgttgaaa ttatagattt aagaacttta tatccattag ataaagagac tatagtaaag
    19561 agtgtatgta aaactgggag agttttaatt tgccatgaag cagctaagac tggtgggcta
    19621 ggtggagaaa tatcagccct gataacagaa agtgaatcat ttgattattt agatgctcca
    19681 gtaaaaagaa tttgtggaaa agatgtgcca ataccatata atccagagtt agaaaaagca
    19741 gttgtcccaa gagttgatga aatagaagaa gcaataaaat ctttaattgt tagataagga
    19801 gggatagata tggtggcaaa taaaataaaa gccacaccag cagctagaag tcaggcaaga
    19861 aaagacaata taaaattaga taggctgatt ggaagtggag aaaatggaag gattcattta
    19921 gtagatgtgt taaattattt aaaagataat aaagcaaata ctactccttt agctagaaga
    19981 atagcagaag atttaaatat agatttagaa actattgtag gtactggata taatggaaag
    20041 ataagaaagt gtgatgtgga aaaattaact ggaaaagaaa ctattgtatc aactaataca
    20101 tctaaatcta gtgagaaaaa agagctgaaa attgaaaatg aaaattcaag aatgtttaac
    20161 acagtagaag gaatatttga aaagccaaat ccaatgagag ctactgtagc aaaacgaatg
    20221 tcagaaagtt attttagtgc tcctgtattt acattcaata tagaagtaga tgctacagaa
    20281 ttgaaggtac ttagagcaaa gttaatcgac acagtaaaag aatcaactgg agtaaaatta
    20341 actatgacag atttaatagt aatggcagta tctaaaatac ttccaaatca tcaagcctta
    20401 aattcagcat ggacagatga gggtatattt agatataaag atgtaaatat agctattgca
    20461 gttggtttag atgaaggctt atatgttccg gtagtaaaaa atgcaaacaa aaagtcttta
    20521 aaagaaatag caaaagaaag caaagaactt gcggaaaaag taaagacagg gaaacttatg
    20581 ccagcagacc aagaaggtaa tacatttaca ataagtaatg ttggaatgta tggtataact
    20641 acatttacac ctataataaa tatgccttca agtgcaatct taggggtagg tgctacacaa
    20701 gataagtttg ttccagtaaa tggagaggct aaaataaaac ctataatgaa cttatcatta
    20761 acatcagacc atagagtaat agatggaaca gtagctgcaa aattcttaaa agatttaaaa
    20821 gaattgttag aaaatccttt atcaatgctt gtataggttt tgatattttg ttactgatat
    20881 aaatagtttg aatagattat attattataa ttgtttcagt aaatttaaag cttttatata
    20941 ttaagaatta tttttaacag taactatatg tgaatatgat ttgcttaatt aaaatatatg
    21001 aaaataaata ggaggagagc ctatgtcagt agaagtaata atgccaaaag caggagtagc
    21061 catggaagaa ggtactatag tatcttggct aaagcaagaa ggcgaagaag taaagattgg
    21121 agaacctata ctagaaataa caacagataa agttaatatg gagatagagt cagaaggaga
    21181 aggtacactt gctgtaatca tacataaaga ggaaggtgaa gtattaccag tatttacagt
    21241 aataggtgta atagcagaaa aaggggaaaa tcaagaggaa gtaaaagcta aatatttatc
    21301 tggaaatgtt tcaaaagaag atatagttga agagaatcaa aatatagaat ttaaagaaga
    21361 aaaaataaac aaaaaagagt gtaatcatga ttatgatgta gtggtaatag gaggaggacc
    21421 tgggggatat ttatcagcat taaaagcagc tcttttaggt ggaagagttg ctttagttga
    21481 agaaaatata ctaggaggta cttgcttaaa tagaggttgt atccctacaa agacttatat
    21541 aaaaacagca gaaatattag aagaaattga ccaattatca aagagaggtg taaaagtaac
    21601 tgtagataaa gaacaggaca ttaagaaggc cataaaatat aaaaatagag tagttaaaaa
    21661 acttacagct ggtgttggcg gattattaaa aagtagagat gtagatgtgt ttaatcttaa
    21721 agctagtgtt aaagaagagc ataaagtaat tttatcagat ggaaaagttt tagatacaga
    21781 aaatataata attgctacag gttccaaagt aaggatatta cctataaaag gaatagaatc
    21841 aaatttaata ataactagta cagaagcatt agatttggaa acagtgccag aagaattagt
    21901 aataattggt ggtggagtga ttggttgtga atttgcagag atattcaatt caagaggttc
    21961 taaagtaact atagtagaaa tggaagatag agtaatccca agaatggata aagagttaag
    22021 cgaatcttta aaatattctc ttagtaaaaa aggtataaat gtattaacta aaaagaaagt
    22081 ttctgaattt aaagaagaag gaaataacat attagtgtgc attgaaggtg aagagccaat
    22141 aaaagcagat ttatgtctat atgctatagg aagagaagct aatctttctg gcatagagga
    22201 cttagatatt aaaatagata aaggttcaat agttgtaaat agtaaaatgg aaacaagtat
    22261 accaagcata tatgcagtag gtgatgttac tggaggtgta atgttagctc atgctgcttt
    22321 taaaatggga gaggtagcag cttcaaatgc tctaggtgtg aataaagaag ttgacttagg
    22381 tgcattacct agttgtgttt atacaattcc agaagtagca tcagttggta ttacagaaga
    22441 ggatgctaga aaaaaatata atgtaaaagt tggtaagttt aattttgctg gaaatggaag
    22501 agctttagct tctggtcaag aacaaggata tgtgaaggta gtagcagatg ctaaatatgg
    22561 agaaatttta ggtatacata tgtttggatg tggtgtagca gaacttataa atcatgcagc
    22621 atcatttaaa gcgttagaga tacctactga tgaagcaagt gagttgatat ttggtcatcc
    22681 atgtacttca gaagctttaa tggaagcttt ggcagatgta aatggagaat gtttacactt
    22741 acctaaaaaa taaatctaaa cataaattat taatttaagt ctattattaa tattaattta
    22801 tatcataaaa ttttaaaaca cttacttagc catacccctt attatgcaaa taatttattt
    22861 gcataataag gggtattttt tatggaaaaa tatagaatat agaatccttg tagtttttgt
    22921 tgtacaaaaa agatagtaat ataaaataac attatcattt tagaatgatt tcaattgtaa
    22981 ttaggaaaag tgattttttt gtttttaaaa aaagtattat ttgtaaaata taaaaaaaaa
    23041 tttcttaata tttgaataaa agaatagctt aatttaaatg ttattacttt ataaaaaaat
    23101 aataagtaca aaaaaaacaa tatatggcat ataagttgct atataaataa taaggaggga
    23161 acaattataa atgaggcaaa gaataaaaaa cttaattgaa aatgaagata agaaaaatcc
    23221 tttaacagat gaagcgattt caagtaggtt aaatattcga agagaagatg ttaccttttt
    23281 gagaaatgag ttaaaaatag aagattcaag acaaagaaga aaagttgttt taattaaagc
    23341 tataaaagat attttaaaag aagaaaaaac tataaataaa aatgatataa caagaagatt
    23401 aaacagcatt gggtttacag tatcaagatt tacagtcata caatacttaa aagagattca
    23461 agattctggt gacctaaaac ttgataaaga gagtgatgat aaaaaagaag aaataatcaa
    23521 taatagagaa aataaagata tagcatttga taaacttata ggtagtagtg gtagcttaag
    23581 tacaattata aaactagcca aagcagcaat tctttatcca cctcatggtc ttcatactat
    23641 tattatgggg ccaactggtg ttggaaagag tgagttagca gagtgtatgt ataaatttgc
    23701 agtagaatca tccagattcc ctaagaatgc accatttata gtttttaatg cagcagatta
    23761 ttcagagaat ccacagcttt tgctatcaca gttatttgga cacgttaaag gtgcttttac
    23821 aggagctgat gagaacaaag aaggattagt atcaaaggca gatggaggaa tattgtttat
    23881 agatgaaata catagattac ctcatgaagg tcaagaaata ctatttcaac tgattgataa
    23941 aggtatggtc agaaagttag gagaaacaag actaacacat aaaatagatg taatgattat
    24001 ttcagcaact tctgagccaa tagattctca tttacttaat acatttaaaa gacgtatacc
    24061 tgtaacaatt gaaataccag aattagttgc aagaccatta aatgagaggt ttgacattat
    24121 aaataatttc ttcttaatag aagctcaaag aatgggagca aacatacata taaagtcaga
    24181 tgtattaaaa gctcttatgc tttacgattg tataggtaat gtaggtcaac ttagaagtga
    24241 tattcaagta gcttgtgcta ggggactatt aaaccaactt acaaacaaac taaaagaagt
    24301 aaatataagc atatcagatg tacctggata tgtaaaacaa ggtcttataa aaatcagaaa
    24361 ttgtagaggg aaaatagaaa attatgtaga tggggattta attatagact caaatcttca
    24421 aggtgagata gaaaagaaaa atgaagatat atatactttt ccagatgaaa tatacaaact
    24481 tactgaaaga agacatattg atttattgga acaaggttta gattttgatg ttataaatag
    24541 aatcattgga ggagaattag aaagttgcat tcaaaaatac ataaaacaag ttaaaaaagt
    24601 ttctattagt aacaatcttc cagatataag tccaattgtt ggaaaatata tcgtagagct
    24661 aacttatgaa gtaatcaaaa tagcatcaca atacctaagt tcactagaac cgaatctaca
    24721 tttatgttta gcagttcacc taaaagcagc ttttgagaga ttaaaagaag gaaaagtaat
    24781 atcaaatgct catttgaaag aaataatagg aaattatcca gtagaatatt ctgtagcaga
    24841 aaaggtagct caatatttaa aggaaagata taacataaac ttaccaaaag aagaaatagg
    24901 tataatagca atatatttta aaatgagtag tagaagagat atgtctgaac aaaaaaagat
    24961 aggcgtactt cttatggctc acggaagagt agcatcttct gtatgtgagg ttacaaataa
    25021 actattagga attaatcacg caaggtattt agatatgcca tttgagaaga agccggaaga
    25081 aatgattgaa gaagctacta caataataaa agatattgat gaaggtagag gtgtgattat
    25141 attagtagat atgggttcac tttcatattt tggagatatt atttctggaa agactggaat
    25201 taatataagg acaatatcta gatttgatac attgctttct atagaggtta tacgaagagc
    25261 agttttacca gatttaacaa tagacgagat agttgatgaa gttaaaggta tagagccaca
    25321 agtaaaagaa aaggttagta aatgtaacaa tacaaaagta gattttagaa aacctataat
    25381 tttaacatta tgtattactg gattaggggg agcttgtaag ataaaaaaaa tcttagagga
    25441 aaatattaaa agtataactg aaaaggttga tatagaacct ataggtatga ttgaatatcc
    25501 aagtcttgat ttagagataa aacaaataag caatgataaa aatgtaatcg ccatagttgg
    25561 aacaattgac cctcagatag caaatatccc tttttttagt ctagtagaca taaatgataa
    25621 agcgaagtta aatcaaataa ataacctaat tgatacaaat caaattggtg aaacaaataa
    25681 aaaatatgaa ttatcagatt taatacataa agaagatata tttgtggatt tacaggttaa
    25741 atcaaaagaa gaagttctaa aatatatgag taaaaaactt agagagaaag gttatgtgac
    25801 aaaaaatttc tataaaaaag tagtggaaag agataactta tttccaactg aattatcaga
    25861 tgaaatagca atacctcaca cagatggaat agatgtaatt aggccagcta taagtatagc
    25921 catcttaaag aagcctattg tgtggagtaa aagtaaagta aaaataatac ttttattagc
    25981 tgtagaccaa aagtgtttta atccattaag cactctactc tcatttatag aaacagatga
    26041 atttaagaag ataaaaaaca ttaaagatag agattttatt agggagatga tattagatgg
    26101 agtcaatgaa aataattagt agcaatttaa tttttaaaaa tattgaagtg tctaacaatg
    26161 aagatgcatt aaagtttttg ggacaacgtt tgtttgacga acagtatgta aaagaaagtt
    26221 acatacaagc tgttgtagca agagaaaaaa agtatgctac agggttacca acagaaatat
    26281 acggagtggc aataccacat acagatatag tacatgtaaa tgagccagga atagcaattg
    26341 gaatactgaa taaacctgta aaatttataa tgatgggtac agacgatact gaaattgatg
    26401 taaaagttgt atttatgtta gcagtgaaag agccacaaga acaattgcaa ctattagaaa
    26461 aacttatgac tatatttcaa gataagaata tactaaataa tattgttgac ttaagtgaag
    26521 aaagtgtgag tgatttatta aactctaaat tgaaaaatta aacatgtaaa gtatattaaa
    26581 aatgtaaaat taaacatgca aagtatgtta aaaaatataa aaagattatt aaagatttct
    26641 aactatagat atctttaaaa taaaataatt tcaatggggg tgactaatgt ggctaaaaag
    26701 atattagttg catgtggaac aggagtatgt acttcaacaa ttgctataaa caaattaaag
    26761 aaagcacttc aagacattgg taaattggat atggttaata ttacacaatg taaagtagta
    26821 gaagttgcat caaaagcacc tgattatgat ttaataattt gtactacaca agtatctagt
    26881 agtataaaaa caccagtgat aaatggatta ccttttttaa ctggtgtcgg aatggataaa
    26941 ttaatcaatg atgtattaga agagttagaa ttataattaa aaaaattaag gaacatggtc
    27001 tctaaaatca taagttttag agacttaaaa ttaaataatg gagggtcttt atatggagat
    27061 attaaatttc attgttggtc taggtgcaca agtaatgatg ccaataataa tatgtatttt
    27121 tggtttgata ttaggtacaa aactaggaaa atctttaaga gctggtttaa ctgtgggagt
    27181 tgggtttata ggtcttaaca ctattatagc tttgctaaca gataatttag gtccagctac
    27241 acaacaaatg gttaagaatt tagggctaag tttatcaata atagatgtag gatggccagc
    27301 agcatcagct atagcttttg catcaacagt aggggcttta attattccaa taggtcttgt
    27361 ggtaaatata gttatgctaa ttactaatac tactcaaact gttgatgtag acatatggga
    27421 ttactggcat tttgctttta caggagcttt agtggcaggt gctactcaaa gtgtaatgtg
    27481 gggagtattt gcatcagtag caaatatggt aattgtattg gtaatggcag atttaactgc
    27541 tccaggtata gaggaatatt taggtatgcc aggtatatct cttcctcatg gatttacaca
    27601 ggcatttgtt ccaattgcta tagtagtaaa taaattatta gacctaatac caggtataaa
    27661 taagattgag ataaatgctg atacattgca gaaaaaattt ggattatttg gagaaccttt
    27721 aataatgggg tctgtaatag gtgtaataat tggtattgct gcgaaatatg atataaaagg
    27781 aatattacaa ttaggtgtaa ctatgggagc tgtattaata ttaattccaa agatggcagc
    27841 attattaatg gaaggattat taccagtatc agaagctgca caggaattta ttgagaagag
    27901 atttaaaaac agaggcaaga tttatatagg cttagattca gcagtaggaa taggtcatcc
    27961 tgttacatta tcagttgcac tagtgttggt tcctttgact atacttatag cagctatatt
    28021 gccaggaaat aaagttttac catttgcaga tttagcagta attccatttg cacttgtatt
    28081 aatcgtacca attacaaagg gaaatgtgtt tagaacatta ataataggaa taattataat
    28141 tacttctgga ttattaatcg caactaactt agctccatta tttacccaaa tggctttaaa
    28201 tgctagtttt aaaatgcctg aaggagcaac tatgatttca agtatttgtg atggtgcgaa
    28261 tccattaagt tgggtatttg taaaagttat gaactataaa gttattggag gagtagtttt
    28321 tggagttata gctttaggaa tggctattta caataggaat agaataataa atgaaaataa
    28381 aaagctatct atagaagaat aaggttaaaa caattttatt aaggagtgta taggttatga
    28441 aaaaatataa atatgattct aaaagatact tactcagtgt tacaatacca ggatttttta
    28501 tgattttaat attgatttat gcactgttta ataactatac aaattttaat ttgaatatat
    28561 atagtttgtt aataattgtc tgtacttatg gtatattcaa tacttttata tcatcaagta
    28621 atcctaaaaa aattattatt gatagcaaat gtatacattt tacctcattt atgactacac
    28681 ataagtatga aattaaagat atagaaaaga taagaataag agaattttat aataaaaaaa
    28741 tttatgtaaa aattaatgat ggaaaccttt ttaaaggtag gtattggata agaacaaata
    28801 tgtttaataa tagtatagca ttatatagct atttcattga gttggaagag tgcttatatc
    28861 cagattcact aaaatttaga aataaaaaat ttgagaataa gaacatataa aaataaaaaa
    28921 ttgaattgaa gcctagctta atcttttttg ataagatata agtttgaact ttatactaaa
    28981 tttttagaat tttcaatcaa gacaataaca taatagctaa gcaattagaa cacaattaag
    29041 atgtataagg catgtatatg atgaatggga ggatttaatt aatgttatta gagaaattaa
    29101 gaaaagaagt attacaagca tctttagacc ttttaaacta taatttagtg acattaacag
    29161 gaggaaatgt aagtgggaga gatgaacaaa ctggctatat agcaatcacc ccaagtggaa
    29221 tggattatag aaatcttaca ccaagtgata ttgttatagt agatgtagat ggaaatatta
    29281 tagatggaaa atggaaagca tcagtggatt tatcagacca cttgtatata tataagcata
    29341 gagaagacat aaatagtata attcatacac attctacata ctcaagttgt tttgctattt
    29401 taaatgaacc tatagaatgt gcaagtacaa ctcttgcaaa tgaagtaggg ggttcagtac
    29461 cagttgcaaa attttcacca ccaacatcta aaaagatggg aaaatgtgtt attgaagcaa
    29521 ttggtgacaa aagagcatgt cttttagcaa atcatggcgt aattgcagtt ggtccatctg
    29581 taggacatgc tcttacagcc gctgtaatgt tagaagattc agcaaaagta tattacttag
    29641 caaaatcaat tggcacacca gtgctactgc ctgatgaaga aattcaaaga gcgagagatg
    29701 tattttttaa tgtctatggt caggataaat aaattagata aaattttaaa agtaatatga
    29761 aaatatagat attcaatgat attaaggaga tttataatga tataataagt ttaatagtta
    29821 agaagtaaaa tatgaaatgt aatagtatat aaattcattt aagactaaaa agaataagat
    29881 aaaataaaaa ggagtctgta tttctatgta ttacttatta aacaaaagca caaatcctta
    29941 ctttaatttg gcattagaag aatatctatt tttaaatgat aaatataatg atgatattat
    30001 aatcatatgg agaaatgaag aatccatttt tataggtaaa aatcaaaatc catatcagga
    30061 agtatatcat gatgttattg aaaagggaga aattcctatt ttaagaagaa tatctggagg
    30121 aggaactgtt tatcatgact taggtaatat caatatgtca ttcatacaaa aagataggca
    30181 acttcatgag atagattttt tggagcatac taagtttatg caaaagatgc tttcaacctt
    30241 aggactagat gtatctataa ctgaaagaaa agatttattt ttaaatggga aaaaaatttc
    30301 aggctctgca cagtctataa aaagaaaaaa ttctctttat catggaactc ttctttacga
    30361 ttcagattta aataaattaa ctaaatattt aaattcaaat aaagcaactg aatcaaatgc
    30421 aactaaatct gtttcaagta aagttacaaa tattaagttt ttactcgaaa aagatataaa
    30481 ttacttttta gattattgtg ttgaatattt aaaatctaat ataaagaata taaaagaact
    30541 tgaattggat gaagaagata ttaaaagtgt atacaatata ggagaagata aatataaaaa
    30601 gatagagtgg acatatggta aaacgccgaa atttcaagta gtgctaccat ttaataatga
    30661 aagtaaaata aatatacatg ttaacagatg gaaagtatca aaatttttta tttcttatga
    30721 tgataatgta attaatatgg acaagttgtt agatttgaat ttctttaaag aagaaataaa
    30781 agagagtatt ttagaaaatt atccacatta catagattta atcaatttaa tattttaata
    30841 taaaacctct aatttgataa aattgtctta tatcaaacta gaggttttta atattcttgc
    30901 atattaacaa ccattacaaa attgtaaaat taaaaacaac actattgaaa aatcaatata
    30961 tattgtaata aatatatgtg tttttcatta gcaaatagac attaactatt tatacaactg
    31021 aaaatttaat atataattaa tcagatggtt aaaataaaaa tattaaaagt tttaataaat
    31081 gatggttaaa aataaaatac gaggtgatac catgtatagt gttataattg ttgcagcggg
    31141 aagcggaagg agaatgaact tagatataaa caaacagttc ataaaattaa gagaaaaaga
    31201 aattattgct catacaattc aagtttttta tgaaaatata aatatagatg aaatagtggt
    31261 atgtataaaa aaagaagaag aagatttttt taaagaaaat ataataaata agtataactt
    31321 taaaaatata aaaatagcat atggcggaaa agaaagacag gactcaatat ataatggttt
    31381 aaaaaaatta gatgaaaatt gtgatatagt acttatccat gatggagcaa gaccatttgt
    31441 cgaccataga ataatcaatg aatctataaa ggtagctaaa gaaaaaaagg ctgtggttgt
    31501 aggtgttcca gtaagtgata ctataaagat agtgtcagat ggaacagtac aagaaactcc
    31561 agaaagaaat ttgctgtggg cagcacaaac ccctcaaacc tttgaatata atctcataat
    31621 agatgcttat gaacaagcat ataagaacaa ttattatggt actgatgatt caatgttggt
    31681 agaaaatata ggtcaaagtg tcactatggt aatgggttct tatgaaaaca taaaaataac
    31741 tagtccagaa gatttaaata tagcagaaca aatattaaat atggaaaaaa gagatgaagt
    31801 taacagtaga aggagaatta tatgagaata ggattaggat atgatgtaca caaattaaca
    31861 gaggatagaa aacttataat cggtggtgta gagataccac acgataaagg tttgttgggt
    31921 cattctgatg ctgatgtatt gatacatgca ataatggatt ctattttagg tgctctagca
    31981 ttaggagata tagggaagca tttcccagat acagatgaag aatataaggg agcagatagt
    32041 atgaaattat tagaacatgt gtataatcta ataacaagta aaggatataa aattggtaat
    32101 atagacagta ctataattgc tcaaagccct aagatggctc catatattga gagtatgaga
    32161 agtaatatat caaaagtttt aaacacagac attgataata taaacataaa ggcaacaaca
    32221 gaagaaggtc ttggttttac aggagcaaaa cagggaatag cttcacaaag tatttgttta
    32281 ttattgttga caagtcagaa taattaatat attattagta ataagtttaa tggatatcaa
    32341 gtaataggcc tatatagaat atctataatg tatgaattta agtgaaatta atataaatat
    32401 aaaattagct atgataaaga aatagtaaaa tgatgaaatc ttcacagaga gaaaacggtg
    32461 gtgagagttt tcaagaggaa gtctttgaac ctgtctttta gcttaaaggt ataaaaatct
    32521 ttacggtagg aaccgttata ttcccaaaag agagctattc atatagctaa ctagagtggt
    32581 accgcggaaa ttaaaccttt cgtctctata cttgtataga gatgagaggt tttttatatt
    32641 ttcaggaagt taatattcag gaggaatcta ttaatgaaaa tgtcaaaaat gtttatgccg
    32701 acattaaaag aaatcccagc agatgctgag attacaagcc atcaattaat ggttaggtca
    32761 ggcatgataa aaaaaatgac ttcaggtgtt tataaccaat taccaatggg gttaagagtg
    32821 ttcaaaaaaa ttgaacaaat aattagagaa gaattaaata aaaaagattg tcaagaaata
    32881 ctttgtgcag ccttacttcc ttctgaatta tggaaagagt ctggaaggtg gactgctatg
    32941 ggtgaagaaa tgtttagact aaaagacaga acagaaagag agtactgtct tgggccaaca
    33001 catgaagaag cattcactga tataataaga caagaaatta cttcatataa acaattgcca
    33061 ctaaacttgt accaaataca agtaaaatat agagacgaga gaagaccaag atttggtgtt
    33121 atgagaacta agacttttac aatgaaggat gcatatagtt ttgatgtaga tgataaaggt
    33181 ttagataagt catatcaaga tatgtttgat gcatatgtaa gtatatttga tagatgtgga
    33241 ttggagaata gccctgtaca ggcagattca ggagctatag gaggttctac atcagccgaa
    33301 tttatggtta agtcagaagt tggtgaagat gaagttgtat tttgtagtgg atgtgattat
    33361 gcagccaatg tagaaagagc agaatcttgt aatctagcat cacaaaaaga agaaatgaaa
    33421 gaattagaag aggttcatac accaggagca gctacaataa aagagttaga agaattttta
    33481 aaaacttctc cagataagtt tgctaaaact ttagtatatg aggcagatgg aaaaacagtt
    33541 gtagtcgttg taagaggaga tagagaagtt aatgaaatca aagtatctaa tgccataggt
    33601 tctgttatag aatttgctct tgctacagat gatgtagtga gaaaagtaac taatgcagaa
    33661 gtaggatttg caggtccaat aggtataaat gctgactatg tatttataga taaagaaatt
    33721 gttgaacaaa gaaacatagt tgttggggct aataaaactg agtaccatat aaaaaatgct
    33781 aactatggaa gagattttga aggaatagtt ggagatttta gaaatgttca agaaggcgac
    33841 aagtgtatag tatgtggtaa gccacttgaa atagctagag gtgttgaagt aggacatata
    33901 tttaaactag gaactaagta ctcagaatct atgaatgcaa acttcataga caaagatgga
    33961 aaatcaaagc ctatagtaat gggatgttat ggaataggtg ttgaaagaac agcagcagct
    34021 ataattgaac aacataatga tgagaaaggt ataatatggc ctttgtcagt agctccatac
    34081 catgttgtga tagttccagc aaatatgaaa aatgaagaac aaatatcaat agctgaaaat
    34141 atatataatg atttgcaagc tatgggtgta gaagttttat tagatgacag agatgaaaga
    34201 ataggtgtca aattcaatga ttcagaatta ataggaatac ctatgaggat tacagtaggt
    34261 aaaaatatta atgaaggtaa agtagaattt aaacttagac ataaagaaga taaagagata
    34321 attgacatag aagaaataaa tgaaaaagta aaggcagagt ttatcaggaa taatgttagg
    34381 ttaggataat aacttaaata agatattgta tagataatca aagttgtact ttatagacac
    34441 cattttatct atatataagg atataaaatg gtgttttttt ataaaacaaa taaatgagga
    34501 ggtaattaaa atggcaaaaa atgaaaaaca atttgttgaa gaaataacaa agatggaaga
    34561 tgattttcca caatggtaca cagatgtaat aactaagact gacttagttg attatgcacc
    34621 agtaaaaggt tttatggttg taaagccata tggatatgca ttatgggaaa agatgcaaga
    34681 atttatggat aaaaaattta aagaaactgg acataaaaac tgttattttc cattattaat
    34741 accagagagt ttattaaaca aagaagcaga acacgttgaa ggttttgctc cagaagttgc
    34801 atgggtaaca catggaggaa ataaaaaatt agaagaaaga ttatgtgtaa gacctacttc
    34861 agagactata atatgtacaa tgtatgcgaa atggttaaaa tcatatagag aattaccata
    34921 tctttataat caatggtgct cagttgttag atgggaaaaa tcaactagac ctttcttaag
    34981 aacatctgag tttttatggc aagaaggtca tacgttacat gaaacagcag aagaagcaca
    35041 agaagaaaca atacaacaat tagaagtata taaagcgcta tgtgaagaat tacttgcaat
    35101 gccagttgta gctggtcaaa aaagtgaaag tgaaaaattt gcaggtggtg aaagaacata
    35161 tactatagaa gctatgatgc atgatggtaa agcattacaa tcaggtacaa gtcacttcct
    35221 aggtcaacac tttactaagg cttttgatat tacttttgca gatagagaag gaaatttagc
    35281 taatccatat catacatctt ggggagcatc tacaagactt ataggtggac ttataatgac
    35341 tcatagtgat aacagaggtt tagtattacc accaagagta gctccaattc aagttgttat
    35401 agttcctata gctgctaaaa aaggaaatgt aatggagact gttgataaaa tatatgctga
    35461 tttaaaagct aagggagtag ctgtagaagt tgatgataga gataactata caactggatg
    35521 gaaatttaat gagtgggaaa tgaaaggtgt tccagtaaga gttgaaattg gaccaaaaga
    35581 tatagaaaat aatcaagcta tggtatttag aagagatact ttagaaaagg attctatgcc
    35641 tttagaagga ttagctgatg ctatatgtga tttatttgat gttatacaca atgatatgtt
    35701 tgaaaaagca agaaaacata gagaagacaa tacatctata gttgaaaata tggatgagtt
    35761 tagaaaagct ttagaagaaa agccaggatt cataaagact atgtggtgtg gagatgcaga
    35821 atgtgaagct aagattaagg aagaaacagg agcaactata agatgcttac cttttgaaca
    35881 agaaaactta ggtcacaagt gtgtgtactg tggaaaagaa gcagatagta tggtagtaat
    35941 ggcaaaagct tactaaaata catttaaata aaaactgggg tagaaagtat ttaatacttt
    36001 atcccagttt ttttgttttt tatgagtaat aaaatgtatt tgtaactatg tcataagata
    36061 agtcatagat acttatatta aaaagaatat aatagagaac tatttttaat aattacatat
    36121 taatgaaagc aaaatatttt tgttatatag attttcaata aagcaaacaa taaatataaa
    36181 tgaatattag taaggagtgc ttttaatttt tagaaaataa atgacttaaa agaaagattt
    36241 atgaaattaa aactaggatt ttctgatata ataaacatgt gtatttttaa ataaaaagga
    36301 tattattatt agatttgatt aaaaataaac ttgcaaaaca atgtactagt ttaatattat
    36361 agttatattg aataaaatat caggaggaag taaaaatgaa tgtaagagta aggtttgcac
    36421 caagtccaac aggatttgta catataggta gtttgagaac tgctttatat aactatttat
    36481 ttgcaaaaaa aatgggcgga gaatatatat taagagtgga agatacagac caaagtagat
    36541 tagtagaagg tgctatagag aatatgctta atgctatgaa atgggctggg gtcaaccata
    36601 gtgaaggtgt tatattagat gatagtggaa aagtagtaca aaaaggagaa tatggtccat
    36661 atattcaatc tcaaagatta gatatatacc aagaatatat aaagcaactt ttagatagtg
    36721 gaaaagctta ttactgtttc tgtacaaaag aaagactgga tgaagtaaga gatgctcaaa
    36781 gagcagcagg agaaacagct aaatatgatg gtcattgtaa gaatctttct aaagaagaag
    36841 tagaagcgaa tataaaagca ggaataccat atgttataag attaagattg ccagagaatc
    36901 atacaattaa gtttacagac ttagttagag gagatatgga atttaatact aatgacttag
    36961 atgaccaagt attaatgaaa acagatggat ttccaactta tcattttgca gtagttgttg
    37021 atgactattt gatgaaaatt acacatgtta taagaggaga agaatgggta tcatctactc
    37081 caaaacatgt ttatttatat gaagcttttg gatgggaagc acctgtattt gtgcatttac
    37141 caaatatact taataaagaa aagaaaaaat taagtaaaag acaaggtgat gtagctgttg
    37201 aagactttaa gaaaaaagga tatttaccag agggattagt aaactatgta gcattagttg
    37261 gttggtctcc tgaagataat caagaattgt ttacaatgga ggaattagaa aaagccttct
    37321 ctgtagaaag agtctctaaa agtggtggag tatttgatac tgaaaaatta aattgggtta
    37381 atcagcatta tataaaagat ggtgatgatg cttatctaac agacttagca ataccatttt
    37441 taatagaaga tggatttata acagaagaag aagctactaa taaatatgat ttcttaaaga
    37501 gtatgatatc agtacttaaa gagaaattac aatatgtaaa agaagttact gaacatgcaa
    37561 gtatattttt tggagataaa atagaggtag aaactgaaga gggaagagag ttcttaagat
    37621 tagaacatat acctacttta atagatgctt tgagagaaaa aatagaaaaa acagaggtat
    37681 taaatgctga gtttgttcaa gctatgctta aagaaataca aaaagagtac aaaataaaag
    37741 ggaaaaattt atttatgggc tcaagaataa ttttgacagg gcaaatgcat ggtccagatt
    37801 taccaaaagt aatggaagtt ttaggaaaag aaacttgctt aaatagaata gcttatgtaa
    37861 aaaataatat attatagtca taaaagtccg ctaatttaga agcggacttt ttttgtgtta
    37921 gtatttggtg aaaatatctg cttaagaaaa tgaaggtatt taaggtaata aatttaatat
    37981 ataaaaagca aagcaaaaat ataaataaaa tatgaataat aaactaataa aatattaagc
    38041 atagaagaag ggaatctatt attttagttt gatttaatta ttaagggggt aattactatg
    38101 tatagtaata ttgatgatgt taaaaaggaa ttaaaggagc tatgcttaga atatgttaca
    38161 attttagaaa aattaaaaga tgaaaaaatg attacagaag aaacttttga aaagtgctct
    38221 tctcaaaaaa aaatattttt agaagaacaa tagaggatgg taatagtatt attattatgt
    38281 agtttgaaag atagtacgaa aaatgatagt aatactatta tttattgcaa ttttcaacta
    38341 taatataata tataatgtta agaagataat ttataaaaat acagtgaggt gatgagtgtg
    38401 aaagtttata atacattgac tagaacaaaa gaagaatttg ttccattaga agaaggaaag
    38461 gtaaaaatgt atgtgtgtgg tccaacagtt tataactata tacacatagg taatgcaaga
    38521 ccatttataa tatttgatac tctaagaagg tatttagagt atagaggata tgatgtaact
    38581 tatgtacaaa actttacaga tgttgatgat aagattataa atagaagtca tgaggaggga
    38641 atatctcctg aagaagttgc ggctaagtat ataaaagaat attttgtaga ctgtgatggg
    38701 ctaggaataa aaagagctac tgttcatcct caggttacag ataatataca acagattata
    38761 gagtttataa aagagttgga agataaggga tatgcatatg cagtaaatgg tgatgtatat
    38821 tttgatacaa ataagtttga aggttatgga aaactttcag gtcaaaaaca agaagattta
    38881 gaggcagggg ctagaattga ggttaatgac caaaaaagac atcctatgga ctttgttctt
    38941 tggaaggcaa aaaaagaagg tgagccagga tgggatagcc cttggggaga aggtagacca
    39001 ggatggcata tagaatgttc tgttatgtct aagagatatt taggtgaaac aattgatata
    39061 catgcaggtg ggcaagattt aacattccca catcatgaaa atgaaatagc tcaaagtgaa
    39121 gctagaagtg gaaaaacatt ctcaaaatac tggatgcata atggatatat aaatataaat
    39181 gatgaaaaga tgagtaaatc aaaaggtaat ttctttacag taagagatat atcaaaatta
    39241 tatgatttag agatagtaag atttttcatg ttatcagcac attatagaaa tccagtaaac
    39301 tttagtgatg agatgttaaa tcaagctaaa gcaggtcttg aaagactata taatactaaa
    39361 gaaaaattag aatttacgct aagtaattta gtagaatctc ctttaacaga aaaagaagta
    39421 gagcttgtta aagaattaga tgactttaga caaaaattca tagatgctat ggatgatgat
    39481 gttaatacag cagacgcagt aagtgtaata tttgaattag ctaagttaat aaattctaat
    39541 gtagatgaaa attcttcttt ggagtttgct aaaaaatgct tagatgaatt taatgaactt
    39601 acaggagtat taaatattgt aaataagaaa aaagatactg tattagataa agacattgaa
    39661 gaattgatac aaaaaagaac tgatgcaaag aaaaataaag agtttcaatt agctgatgat
    39721 attagacaac aattactaga tatgggaatt gtattagaag acacaagaca aggtgttaag
    39781 tggaaaagga tataaaaatg gaaaaaactg aattagttac tatgtcacca ttagttttag
    39841 catacttagg tgatacagtg tatgagacat atataagaga atatttaatt agacagaata
    39901 cacaaagaaa agttaatgat ttgcataaac tagctataaa gtatgtaaaa gctaaagctc
    39961 aggcaactat aattcatgaa attgaaattg agttaactga agaagagagt aaaatttata
    40021 aaaggggaag aaatcaaaag tcaaatactt ctcctaagaa tgcagatatt atagactaca
    40081 aacatgcaac tgggtttgag gcattagtgg gatatttgta tttaaataat gaaatagaga
    40141 gacttcaata tattatcaat aaaggaatca aaattattga aagagatatg taaatattaa
    40201 agagtgttgt cttaataata agtaataagg caacactttt tttataacta atgtataata
    40261 aatatataga aattagtaat gagaaaggat aaaaagttat gaaagtaaaa ttaatatcac
    40321 atacacctga accagaaaaa gtaatagcta tggcagctaa gctatgttat tcaccagttg
    40381 ggacagacga aatagaaaaa gacttaacag atgaaagtat agagaaattt ttaaatatgt
    40441 tattaagtat aggtcatggg tcaatattag aacatgcttc atttacattt tcaatagaag
    40501 gtatttcaag ggcttgttca catcaaattg ttagacatcg tatagctagt ttttcacaac
    40561 aaagtcaaag atatgttaaa ttagaacaat ttgagtacat aattcctcca gaaatagaaa
    40621 aaatagaaaa ggcaaaagag ttatttatag actccatgaa aaaagaccaa gagaattatg
    40681 ataagttagt tgaaatattg tttgaaaatc attataatga cctaataaaa aacggtaaaa
    40741 atgagaaaac agctaaaaga caagctgaaa agaaagcaat agaagatgct agatacgtat
    40801 ttccaaatgc atgtgaaaca aaaatggtat ttactataaa tgctagaagt ttgtttaatt
    40861 tctttgaaca tagatgctgt gaaagagcac agtgggaaat aagaaattta gctgtggaga
    40921 tgttaagaga ggttaaaaag gttgctccta tattatttaa aaagaccggt ccaagttgtg
    40981 taaatggaag ttgtccagaa ggaactatga cttgtggaga tatagttcaa gttagagaaa
    41041 aatttaaggc cttgtaggag gtgaataatt tggcgagtat agaaggaaga aatccggtaa
    41101 tagaagcaat aaaaagcgac agagaaatag ataaaatatt aattgcaaat tcagctaaag
    41161 aaggttcaat taagaaaata ataggaatgg ctaaagataa gaatataatt attcagtatg
    41221 ttgataaaca taagctagat gaagtaagta caagtcattc acatcaaggt gtaatagctt
    41281 atgcaagtga gtataagtat tatgaattag atgagctaat agatttagca aagaataaag
    41341 atgaagaccc atttttcatt atattagatg agataacaga cccacataat ttgggttcaa
    41401 taataaggac agcagatgca gtaggagcac atggagttat cattccaaaa agaaggtctg
    41461 tacacataac tcctgtagtt gccaaagcat ctgctggagc agtagaatat atgccagttt
    41521 gcaaggtaac caatatagtt aatacaataa aaagactcaa agaagaaggg ctgtggattg
    41581 ctgcagctga tatggatgga gaaacttttt ataaacaaaa tcttactggt cctcttggtg
    41641 tagtaattgg aagtgaaggt tttggaatat ctagattggt taaacaaaac tgtgacttta
    41701 tagtaaaaat gccaatgata ggtaatgtta catctttaaa tgcatctgta gcaggtggaa
    41761 tacttttata tgagatattc agacaaagac tggataaaag taaataggca tgaaaaaaaa
    41821 tataaatcat tacttaataa tagatggcta caatattata aatgcgtggg acaatttgaa
    41881 agaactggct aaagaggact tagaagattc tcgagaaaag cttattgatg acattataga
    41941 attttctgag tttatgggtt ataagactat aatagttttt gatgcttata atgttaaaaa
    42001 ttctcgagaa aaagttgaaa aaagaaaaca cataacaata gtttatacaa gagaacacca
    42061 aacagcagat agttatatag agaaatttat aacttcttta tcaaagtatg atgatgtaaa
    42121 agtagccact aatgactatg cagaacagca aatgatttta ggaaaaggtg ctactagaat
    42181 gtctgcaaga gaattaaagt tagagttaga tcgttccaag aataaaatga aggaaaaaaa
    42241 tatcggtttg aggaaaaaaa ttcagcgaaa ttggttggag gaaaggttag ataaagaaac
    42301 attgtcgaaa cttgagaaca ttcgtagaaa gcgttgaaat tactaggttc tttagactat
    42361 aatataccta taaatttgtt ttatatatgg gggagattta catgttggta gcaaaagaaa
    42421 aaagttatga gttagtggac aattgtcagc aggatgagta taacattgta ttaagagcaa
    42481 gtgaagggga taaaatagca ctagagtaca ttattactaa atacagaaat tttgtaaagg
    42541 caaaagcaaa gtcatatttt ttaattggag cagataaaga agatataata caagaaggaa
    42601 tgataggcct ttataaggca gttagagatt ttgatggaag taagaccaat tcatttaaat
    42661 gttttgcgga gatatgtata acaagacaaa taataactgc tataaaaact gcaacaagac
    42721 aaaaacatat acctttaaat tcatatgttt ctttgaataa accaatatat gatgaagagt
    42781 cagacagaac acttttagat attatagcta caagtatagt aacagatcca gaagagctta
    42841 taattagtaa agaagagtta aaaaatatag aatcaaaaat gaacgaattg ttaagtgact
    42901 tggaattaga agtactagaa ttatatttaa atggaaagtc ataccagttt atagcggaca
    42961 agcttaaaag agatgtaaag tcaatagata acgctttaca aagggttaag agaaaattag
    43021 aaaaacatct tgaaaacaga aatgattaat agtaaaatac ttaccataag cttattgtga
    43081 gcaaaattaa tttatatcga aagataaggg aggaaattca aaatggctaa agctaaatac
    43141 gaaagaacaa aacctcatgt taatataggg acaataggac acgtagacca cggtaaaact
    43201 acattaacag cagcaataac aaaaacatta tatgacagat atcaattagg agaagcagta
    43261 gatttcgcaa acatagataa agctccagaa gaaagagaaa gaggaatcac aatatcaaca
    43321 gcacacgttg agtatgaaac accaaat
//
LOCUS       DBGJFU010000018        43205 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.018, whole genome shotgun sequence.
ACCESSION   DBGJFU010000018 DBGJFU010000000
VERSION     DBGJFU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.018"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(26..256)
                     /gene="dltC"
                     /locus_tag="ACOIAS_000950"
     CDS             complement(26..256)
                     /gene="dltC"
                     /locus_tag="ACOIAS_000950"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HGZ2700669.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(275..1423)
                     /gene="dltB"
                     /locus_tag="ACOIAS_000951"
     CDS             complement(275..1423)
                     /gene="dltB"
                     /locus_tag="ACOIAS_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HGZ2700670.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(1423..2937)
                     /locus_tag="ACOIAS_000952"
     CDS             complement(1423..2937)
                     /locus_tag="ACOIAS_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HGZ2700671.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(3058..4248)
                     /gene="dltD"
                     /locus_tag="ACOIAS_000953"
     CDS             complement(3058..4248)
                     /gene="dltD"
                     /locus_tag="ACOIAS_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HGZ2700672.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(4536..5555)
                     /locus_tag="ACOIAS_000954"
     CDS             complement(4536..5555)
                     /locus_tag="ACOIAS_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGZ2700673.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(5731..6270)
                     /locus_tag="ACOIAS_000955"
     CDS             complement(5731..6270)
                     /locus_tag="ACOIAS_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HGZ2700674.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(6371..7051)
                     /locus_tag="ACOIAS_000956"
     CDS             complement(6371..7051)
                     /locus_tag="ACOIAS_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2700675.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(7080..8369)
                     /locus_tag="ACOIAS_000957"
     CDS             complement(7080..8369)
                     /locus_tag="ACOIAS_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGZ2700676.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(8818..10419)
                     /locus_tag="ACOIAS_000958"
     CDS             complement(8818..10419)
                     /locus_tag="ACOIAS_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2700677.1"
                     /translation="MIYDLILKNGFIIGGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIAKQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(10530..11207)
                     /locus_tag="ACOIAS_000959"
     CDS             complement(10530..11207)
                     /locus_tag="ACOIAS_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700678.1"
                     /translation="MSKVKSIYNEEYLPFKIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTMLIARAM
                     YKDKLSD"
     gene            complement(11220..11915)
                     /locus_tag="ACOIAS_000960"
     CDS             complement(11220..11915)
                     /locus_tag="ACOIAS_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HGZ2700679.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(11967..13166)
                     /locus_tag="ACOIAS_000961"
     CDS             complement(11967..13166)
                     /locus_tag="ACOIAS_000961"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HGZ2700680.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(13427..15472)
                     /locus_tag="ACOIAS_000962"
     CDS             complement(13427..15472)
                     /locus_tag="ACOIAS_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2700681.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(15883..16692)
                     /locus_tag="ACOIAS_000963"
     CDS             complement(15883..16692)
                     /locus_tag="ACOIAS_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2700682.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQKFLQFLEE"
     gene            complement(16776..17309)
                     /locus_tag="ACOIAS_000964"
     CDS             complement(16776..17309)
                     /locus_tag="ACOIAS_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGZ2700683.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(17459..18808)
                     /locus_tag="ACOIAS_000965"
     CDS             complement(17459..18808)
                     /locus_tag="ACOIAS_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HGZ2700684.1"
                     /translation="MELKNFKDILFKKALSEGFEEYEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(18820..20202)
                     /locus_tag="ACOIAS_000966"
     CDS             complement(18820..20202)
                     /locus_tag="ACOIAS_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HGZ2700685.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            20446..21660
                     /locus_tag="ACOIAS_000967"
     CDS             20446..21660
                     /locus_tag="ACOIAS_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2700686.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFNFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            21837..23750
                     /locus_tag="ACOIAS_000968"
     CDS             21837..23750
                     /locus_tag="ACOIAS_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2700687.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            24021..24995
                     /locus_tag="ACOIAS_000969"
     CDS             24021..24995
                     /locus_tag="ACOIAS_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HGZ2700688.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIIALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            25016..25291
                     /locus_tag="ACOIAS_000970"
     CDS             25016..25291
                     /locus_tag="ACOIAS_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HGZ2700689.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            25304..26467
                     /locus_tag="ACOIAS_000971"
     CDS             25304..26467
                     /locus_tag="ACOIAS_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HGZ2700690.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            26804..29083
                     /locus_tag="ACOIAS_000972"
     CDS             26804..29083
                     /locus_tag="ACOIAS_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2700691.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(29178..30518)
                     /locus_tag="ACOIAS_000973"
     CDS             complement(29178..30518)
                     /locus_tag="ACOIAS_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2700692.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(30530..31312)
                     /locus_tag="ACOIAS_000974"
     CDS             complement(30530..31312)
                     /locus_tag="ACOIAS_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700693.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(31327..32349)
                     /locus_tag="ACOIAS_000975"
     CDS             complement(31327..32349)
                     /locus_tag="ACOIAS_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGZ2700694.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(32355..33368)
                     /locus_tag="ACOIAS_000976"
     CDS             complement(32355..33368)
                     /locus_tag="ACOIAS_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGZ2700695.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTKFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(33496..34434)
                     /locus_tag="ACOIAS_000977"
     CDS             complement(33496..34434)
                     /locus_tag="ACOIAS_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2700696.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEDKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            34704..35168
                     /locus_tag="ACOIAS_000978"
     CDS             34704..35168
                     /locus_tag="ACOIAS_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700697.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILDGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(35280..35597)
                     /locus_tag="ACOIAS_000979"
     CDS             complement(35280..35597)
                     /locus_tag="ACOIAS_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2700698.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(35610..36341)
                     /gene="chbG"
                     /locus_tag="ACOIAS_000980"
     CDS             complement(35610..36341)
                     /gene="chbG"
                     /locus_tag="ACOIAS_000980"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGZ2700699.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(36367..37677)
                     /locus_tag="ACOIAS_000981"
     CDS             complement(36367..37677)
                     /locus_tag="ACOIAS_000981"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGZ2700700.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(37774..39087)
                     /locus_tag="ACOIAS_000982"
     CDS             complement(37774..39087)
                     /locus_tag="ACOIAS_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2700701.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(39109..39420)
                     /locus_tag="ACOIAS_000983"
     CDS             complement(39109..39420)
                     /locus_tag="ACOIAS_000983"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2700702.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(39620..40324)
                     /locus_tag="ACOIAS_000984"
     CDS             complement(39620..40324)
                     /locus_tag="ACOIAS_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2700703.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCEYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(40415..41776)
                     /locus_tag="ACOIAS_000985"
     CDS             complement(40415..41776)
                     /locus_tag="ACOIAS_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2700704.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            42275..>43205
                     /locus_tag="ACOIAS_000986"
     CDS             42275..>43205
                     /locus_tag="ACOIAS_000986"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2700705.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEY"
BASE COUNT        14757 a         7369 c         5049 g        16030 t
ORIGIN      
        1 taatatttaa agtctaacta ctaatttatt ttcttgtctc caagaatttt actaaattat
       61 ttacagtaga catatctttt ctctctaaat cagttggttg aagctctatc cctaatctat
      121 tttcaatttc aagtaaaact tcaattatag ctaatgagtc taaaagttcc gtctcaaata
      181 aattaaggtc taaatcttct cttatctcat cagtccctaa tacatcttca aatatttcta
      241 taactgtttc ttgcataaat tttcctcctt aaaattaagc taaataacct gagaatatca
      301 gcaatccaaa acacacaata tggaatgtaa tgaaaatctg cactctctcg aaccatttat
      361 cttttttatg ttttttataa aatttagatt ttcgctgata tatatcagtt aacacaagtg
      421 ccaatccttg gtatacacca taagcaacat aataccaagt tagaccatgc caaaatccca
      481 ttgttatcat ggttatcatc tgagccacat gagcagcagt agtccttttc ttaaatcttt
      541 tctttctcat tgatgacata acaaatctag aaaaaatata atctccaaac catcttgaaa
      601 gacttatatg ccatcttgtc cagaactctt tcatatcttt gctgataaat ggcttgtcaa
      661 agtttatagg tacttgtatt ccaaatatgt accctgtacc tatagcaaat aaactatatc
      721 ctgcaaaatc aaagaacagg tataaactat atgcgtacat ataacttaaa atatttataa
      781 aactcatatc tttaggtatt cttgataccc aatatgtatt tatcaaaaat gctataacaa
      841 acttataacc tactcccatg acaatatttt ttattcctgg cagtaagtat tcttctatat
      901 actcttttct agagatttgt ttttccaagt cctgttcaaa tcttcttgac ctatcaatag
      961 gtccagaact taaagttgga aaaaataaca taaaataaag catctttgaa attttaactt
     1021 cttttatagc tccatcataa atttcaataa ccatttgtat agttctgaaa tttaagtatg
     1081 atattccaat aaatccaatt attccaaaag aagtaactgg agatatttta tttattatta
     1141 ttgggagcat tgatgcaaac aagaatcccc aatatatgta tttattttta gtttttcttc
     1201 ttacatattc atatccttta actattatta cttcaagaat aataaatatt accaagtact
     1261 ttagttggac atcaattcct actattaaga atatcataaa caaacttgcc aacataccat
     1321 aatactttat attcttgccc attagaccta aaattactgc tggtattgat gttaataata
     1381 atatataaag ataaaaataa tctccatatt gtgaaaaaat cattaaattt cctccataag
     1441 tttctttctg tctattttac cattgatatt tgttggaaac tcagagatta ttgaaatatt
     1501 tcttggaatc atatatgaag gtatatactt tccaagttcc ttttttatta tcatcccatt
     1561 tttaatatta cttaaatcat tcttttcatt taactcaaca atacctttta agtaagcaat
     1621 tttttcgtcc ttatatactg gtaaaactac tgcatttttt acattatgta cttttctcaa
     1681 attattttct atatcttcta tttcaattct aaatccattt aatttaatct gaaaatcttt
     1741 tcttccacaa taatatatat ttccatctaa aagatatccc atatctccag ttttataagc
     1801 tctccacttt acaccatcta tttcatcata gaaaaatact tcatcagtct tttctttatt
     1861 attgaagtaa cctttagaaa ctgatggtcc aattattata atttcaccct tttcattttc
     1921 tttaagctca tttccatctt catcaagtat ttttatttta caatttgaca tagggtatcc
     1981 aactggaaga cttttttcat catctatagc cttttgagtc atatcattta cacttacacc
     2041 aacagtagcc tcggttggtc catatccatt gataattctt gtgtttggaa atctactcat
     2101 aagttcttta gtaagatttt ttgaaagtgc ttctcctatg aatatcattg actcaaggtt
     2161 aggaagcatt ttactattaa actctttttc agttatacat actcctgcaa aagaaggagt
     2221 tgacacccat acagctatat ctgagatact aaattgtcta aatagttctt tatagtctgc
     2281 taatacatct tttgatattg aaaataatgt agctccatgg ataagaccag ggtatattgt
     2341 tgtaactgaa aggtcaaaag aatatgctgg ttggttcatt attacttttt cacttccatc
     2401 tatgtttaga tatgggctta tccaatctga aaagctatct aaattgtttg aacttatttg
     2461 aacccctttt ggctttcctg tacttcctga agtaaatagt atatatgcat tttcatcatc
     2521 ttttacccag ttttctttat ctaatgattt tccctgatat tcatttatta tataatttaa
     2581 tttatccata tcaattacat tgatatctcc aaaatttctt tcatcactaa agttaaataa
     2641 tacctttggt tttatctctt tagtgacttc aaaaactcta tcaattggaa aactaatatc
     2701 aagaggcaca tacgctctac ctgattttaa agctcctatc atacatgcca ttatcatgtt
     2761 ttctttattt ccataaatta ctataggagt atcttcttct ttgtatacat cctttagaaa
     2821 tactgatata gcatctgaat attcatttaa atctttataa gaaagtttat ctccattaca
     2881 cattagtgct gttctatctg tatttgaata tttttttatt ccttcaataa ttttcatata
     2941 agaatgctcc tttaaaatcc gttataaata aatggcaatt ctttaaatgg agtaaatgtt
     3001 agaactccta tcgccaatat tataaccata gttaaacaaa tactagttaa caaatcttta
     3061 ttgttcttta aatattttaa ataatctctc acagacatct acccatcctt tcgtaccaag
     3121 atgcattaca tcccttaaat aatacttatc tgacccttta tctttaaggt ttagaacttc
     3181 aaagcctttg ctttctgcta ttttttgtgc tttatcgtag tactgatttc tttctttttc
     3241 agaaatacca gtcaaattat agaatttatc catacttggg attaatacaa caactggctt
     3301 aatacctaag tcagtacata catttagcat cagtttataa ttttcaaact ccttagatgt
     3361 taataaattt acatctttgt ctcttccttt gtattgctct ataccatctt taaaatgctc
     3421 tttataatag tacttatcaa tatttagtgg atttttacca actctttttt tagcatcttc
     3481 aatggctttc tttctctcat atgaccaatt tattggtctt tttctttttg ttgctctctt
     3541 tttatctagc ttaattaatc gtttataaag aatacccttt tctttaagag ctatacaata
     3601 ctttcttcct tgaaaataag gctctaaaag caccttttca gctttttcag gaaatgtctt
     3661 aggctcatat agcttagcat ataatgcttc tgctttatac tcgtcacttc cccataataa
     3721 cttacttgat ttttttgcat attcaatctt attttgttta gatatttttg gattatctaa
     3781 aaatcgataa aattgtattg gtgaaaatct agactgataa tggtgacttg tgactccgtc
     3841 tttttccata aaccattgca tagaaattaa caagacaact ttcttattat caatattagg
     3901 attcatactt cctaatattg cagcatcttg taatgtctga gtataggctc tcccaattgc
     3961 aaacacctta tttttactcc tatttgtatt aaaatagtac tttggatgtt gttttgttga
     4021 attactaagt tctgatgacc ccaaaatcat aatatccttt tctcttaaaa aatgattatt
     4081 tgctgtaacc cctttatctt ttatatcact taaagtatca tccataattg gaagtagatt
     4141 tttctccctc aaaagttcat cagttttaga atcaagaaat ttgtctaacc caaataaaaa
     4201 tactacgcct attataaaag gtgtaataaa gtatatcaat tttctcatat tttctctcct
     4261 ctaaaaatat tcaaatttat gttattatta atcatattag ttttgatatg tatattcaac
     4321 tacaaaattg taataaaatc gtcaaaactt tttttgatac tgttacaaaa ttgtaaaaaa
     4381 aaagaaaaaa acgccaaaaa taaatttctt tttagcactt tttcatactt ataatttgac
     4441 attttaacac ttggttaagt atacacctta ccttaactta aaattaaatt tatttaagaa
     4501 tatatattat aaattcttat attttctaat ataaactata tactatcagc tggataaaca
     4561 atactacttt gtcttcttat ctcatccata atttccatta ctattattga attttcatga
     4621 gaatttatat ttgattctat tttattgtta tttataagac ttataaactc tacaagttca
     4681 taatacattc cttctatttc tctaggcttt tcctctgctt tttcttttgg aatagatata
     4741 tcttcttctc ttccatctct gtatttaata atagcttttt caaatagatt aagcttttca
     4801 attattatac ttccctcttc tccctgtatt tcagatggta tataagaatc agctacttta
     4861 gaatactgaa ttatcgcatc catatcatca tattgaaata cagctgaacc ttctccatcc
     4921 actccagttt gaagtatatg agaaactgcc tttacagttt ttggagcacc aaataaattc
     4981 accataggat gtatacaata cactcctata tccattaaag ctccatttga caactctttt
     5041 ttaaaggcat tttctataat tccattttta tatttatcat atcttgaaga atactgacaa
     5101 tagcttccaa aaaatcttct tattttccct attttataca agttttcttt aactgcttta
     5161 aagtttggaa cacatgtaat tctcatagct tccataagca atacattatt atctttggct
     5221 gtttttatca ttgatttgac ttcttttaaa ttagatgcta acgacttttc acaaagcaca
     5281 tgttttttgt gttttaaaca agtaatggtt tgctttgaat gtaatgcatt tggagaagct
     5341 atgtatacag catctatatc cttcgattta gccatttgtt ctaaatcatc aaaaaaaata
     5401 cttgctccat atagagttcc aaattctcta gctttttcta aatttctaga atatacagca
     5461 accaattcaa agtctttaac cctagatgca gctcttagaa atatatttgc tatattgctt
     5521 gtcccaacaa taccaaatct tattttaccc atcataaatc actccccatt ttctacactt
     5581 tattttaatt ttaataaaaa actttacaac ttatagatat attataaaat aattttatca
     5641 aataaaaaag aaagctaatt ttaaatgatt tcaccatttt aacatagctt tcttttaact
     5701 acaatctaaa ttcaacttat atttttaaag ttatctttct actatatctt tatttcccct
     5761 caaaaatttt atagtaagaa tttcatccac aaacaatgtt actaaaagta tagttacaac
     5821 aattggccaa taaggtatta taagactata taaactatca actatataca cttgaaaata
     5881 tctaactcca ataaatgtca atatagtcca atatacagaa aactttagac atactattcc
     5941 tctactattg tacttataat ctgaataatc ccaatagacc ttattaaaat atactttcat
     6001 aagtattcct gtaatatatt ctactactgt cggcactact agacataata aaagcaatgc
     6061 aaatatatta ttggggtcaa taaatcttga gacctctatt ataatcgaca ttgcaattgc
     6121 atacattggt ttaaatggac catttaaaaa tccatcttct tgaaaatgcc cttgtttata
     6181 ataactatat aagttttcaa ttatccaacc tataaaacca tacaagaaaa aattaaataa
     6241 aaaatataat agagaaccta cattatccat gaatattcct cctagctgtt tatacttatt
     6301 ttttctcata ttcatggatt tattctaaat caaagaaatt atatttaatt tatttaatta
     6361 atactttata ttatagattg tgacattctt tagagttcaa actttttagg tattctctaa
     6421 cttccagtaa attttcaagt atcttatgtg attgtgattt aatttgctca tctggctctt
     6481 ttaaatctgg tacatttata catcttatac caccattgta tgcagcttca actcccatag
     6541 gcgaatcctc tatgacaata caatttttgg gattaacatt tatcttttta gctgctttta
     6601 ggaatatctc agggtttggt ttcgagttta caacatcatc tccacaaact atagcatcaa
     6661 agtaatcttt taaatttgcc ttagctaatc tctttactgc tctttctctc tttgttgatg
     6721 ttgcaacagc cattttgtat ccattttcct ttaaaaaaga gattagttca ttgactccta
     6781 atttgatagg agctcccttc ctctccataa attcaatcat atttttagtt ttttcgtcat
     6841 ataaatctat tattggtaca gaactatcgt atatatctgt aagaccttct attattcctt
     6901 tacgatttct tcccataact gatgtataaa tttccttagt catagtatat ccatactttt
     6961 caaacgtctc catccaaaat tcaagagaaa ttctctctga gtcaaacaaa actccatcca
     7021 tgtcaaaaat aattccttct actttttgca tacaattcct ccaatatttt ttgatataat
     7081 tatattacac tttttatgat taattttaca accctcaatt tatccaattc taatattgta
     7141 aaattgtaat tttcaaaagt tacactttgg tttactttaa tatttgaacc aagcatatag
     7201 gatatccatc cacctatact gtcaaatcca tcatgctcta tatcaagtcc aaaatactta
     7261 ttaatatagt tgatagattc agttccatca actaagtaag aacctgctcc tattttctta
     7321 atatgagtct cttctttatc aaattcatct tgaatttctc caactatctc ttctagtata
     7381 tcttctattg ttaccacacc agatgtacca ccatattcat ccacaactat agcaagttga
     7441 agtttttcct ttcttattct ctcaagagct ttatctatag ttaaattttc agatatatat
     7501 ataatatctc tcaatatttc ttctagttca attttatttt catttatctt ttgattgtat
     7561 aaatctctta tatgtacaaa acctaatata tcatctttat tttttctgca tactggatat
     7621 cttgtaacac cttcctcttt taaaatagct aatatctttt cttcagaatc actttcatat
     7681 atacatacca tatcagttct aggaaccatt atctctctaa ttttcttttc ttcaaattca
     7741 aaaatattat caactaatct ttgctctgat tcatctataa gtccactttt ataactttct
     7801 tctactaata gtcttatttc gtcacctgta tggggttcat caacctcatc agcttgtgaa
     7861 tatccaaatg gtttaagaaa taaatcagta cttaagttga aaaaatatat tattggatat
     7921 gttaacttat aaaatcctac taataaaaaa gatgtactca acattatttt ttctgtatta
     7981 taaagtgcta aagctttagg aaccaactct cctattacaa cctccatcat tgttataatt
     8041 aaaaaagaaa tggaaattga tatagtatat ataatacttt cactaagatt aaatacactt
     8101 actattggta aaattaactc tttaactgta gattctccca tccaacctaa tgcaagtgaa
     8161 caaagtgtta taccaagttg acatgcagaa agataggaat ttaagttgtc ttttattatt
     8221 ttacatcttt ttgcattatt attaccttca gttatcatgg tatcaatcct agaactacga
     8281 agttttacca tagcaaattc acaagctaca aaaaaagcat ttatcaaaac aagcattact
     8341 ataagtgtta cttttaataa ctcccgcata aagtataggt tcttataatg taagaaatct
     8401 ataagaactc aaagttcccc ccttatatta ataaatttaa tttataatat atattctatc
     8461 atatttatta atatatccta ctttattaca aataattttt ataaactaaa taaccccttt
     8521 taatatttat gactggctat ttgataaata ttaaaggggt tatttagtta tcatttttta
     8581 tttctaaaaa tcttttctca aatgatttag ttatctattt atccaattct ttacataatt
     8641 taatgattaa aaatattaat tttttcacta taaagtataa atattatctg attattaaaa
     8701 taaatattaa tactataaat aaaaacgaag catgattttt atttaaataa atctcaaact
     8761 tcgtctttac ttacttagat atttatttac tttctaaatc ctattatttt taaaacctta
     8821 tctttttaat acttcaccac atttattctc agtaatctct ccattttcca acgcaatttt
     8881 tccatttaca attacatatt caacaccatt ggctagtaat tgtggctttg aaaaatcaat
     8941 atcttctgac ctagaataac tatagttatc catgtcaata actgctatat cagctatcaa
     9001 cccttcttta agaatacctc tattttttat tttaaattgc ctagctggta atgaagtaac
     9061 tcttcttaca atctcttcca atctaacacc agactttctg tacatattga agaaaattgg
     9121 aaaagctcct cttctttgta gttcaaacca tgacatatcc ccacccacac taaacaaaca
     9181 atctgaacca accataacaa atggattatc aattatttta tcatcatgta gtttatctgg
     9241 gaaatcagct ctatacattc ctcctaaaca aaagataagt tcttcgtctc catctaaaag
     9301 taaatcgagt aataacttgt cttcatctat tcctttttgt gtagctattt cacgtattga
     9361 tttcttttcc atattaatat ctttagtgtt tagaagtatt atactccccc tatctccaag
     9421 tataaatgct tcttttaata taagtgttct tccctcttca tttctaagag catccattac
     9481 cccatttata cctaattcaa atagactaga tgatattgcc ataataaatt gaattactct
     9541 ttttttcttc atacaatgcc cagaacttct tggtactgtg tcaaccatta tttcaacacc
     9601 attataccta gcctttaata tctcatcaaa tgcttctatt tcaactggac ttaagtgaga
     9661 tacttgaacc ctagcaccac ttttttgccc aacctctatg gcttctttta ctgcattata
     9721 tcttcctatg taatctctaa tatgagatgt atatatacca tcatgctctt taatctgctt
     9781 tgcaatatca acaagttcat caatatcagc atacttactt ggtatatatg ccaatccagt
     9841 agatattcca aaagcaccac ttttcattcc atcatttatt atatctgtaa tttcattttt
     9901 ttccttttct gtaggaggtc tatccttaga acctcccata acactccatc ttatagtccc
     9961 atgccccaac aagaatccca tattgacaga aataccactt ttagatataa ggtcacaata
    10021 ttctgtaaaa ttattccaac atttattttt atctataaga tacttttttt cttcttcaag
    10081 taatagacca tttttataca tatattcata tacattttca gaagaatatg gtgttataga
    10141 atgtccacaa ttaccattta tagttgttgt aactccttgt tttagaaact tttcaaattt
    10201 accatctagt attgctacta tttcttcatg aacatgaggg tcaataaatc caggtgttat
    10261 aacttttcca caacaatcaa tctctttatt tgtattgcta tttatcttag aatctatttt
    10321 tgctattaag ttgtctttaa ttgctatatc tccataaaaa ccaggattac ctgtaccacc
    10381 tattataaat ccatttttta agattaaatc atatatcaaa atattccctc cttaaaaacc
    10441 aatttttaaa taatcttaat gtaattttta tatttgaaaa tcaaattatt atatttcata
    10501 tttgaaaatt cactattatt tcattgtaat tagtcactaa gcttatcttt atacatagct
    10561 cttgcaatta acatagtacc aaatacacat ataagaacta ctagagactg tggtattata
    10621 ccttgctttg ataatataat agtcaataca gatattacaa ttgctactag gcctaatttt
    10681 ttatcttgta aaaatacttg tccaaaaact gacccaaata gagctggtaa aattaagttt
    10741 aatttttcta ctacttcagc tggaatttta gatagtacgc ttcctcctaa aattacccct
    10801 atagttagta tacttatatt cactaaaata gatacagcta ttcctattgt agatattatc
    10861 gagccttctt ctgtacctga ttcaacttct gctgcctttt gagctgctat tgaacatggt
    10921 agcctcatat tagatatatt accagaaaga aaactcatat aagttcctgg gatacctaag
    10981 attggtgaat aagaaactgg ttctattatg taatttggag cactcatact gacaattgcc
    11041 attgtacttg ctaataatgc tgatattgga ggcataatac caaatccaaa gctcaaaatt
    11101 attccaggaa ctaatgctgc tataatcccc aaagatagtg ttagtcttcc ataccttatc
    11161 ttaaaaggta aatattcttc attgtatata gattttactt tactcataaa actcccccct
    11221 tatattactg aagctattat cattccgcta aacattgata ttgtaagccc ccactctctt
    11281 aaccatttta tatttttctt cttttcaagc atacacaata ttaccattat agccattcca
    11341 cttatacatg caattgttcc attatcaaaa cgcaacaccc tatcagcatt tagatatgca
    11401 aatgaaccca acattgctcc tagagatatt attggtataa atgatttctt gccactagat
    11461 ataacattgg ttatcttatc tagtttatgt ccaaatagta atgtgaatat tatccatcct
    11521 aaagaaccta atatcatagt ccaaactgca tttgtaaatg caagttttgt catttcagga
    11581 gaacctaaag ttatacctaa tgcatctgtt ccaaatccgg ctgacattga ttcaaaaatt
    11641 acagaaccta taaatgataa tctcatccat gctataggcc cacccatagt tactataagc
    11701 gataccattc cagcaaatat agtcattgaa ggcccaatag ctgaaatagc actactctta
    11761 acagctgact ttatttgttt atctgttatt cccattgttt taccaacttt gcaagatttt
    11821 atagcaaata taagggattg taccactact attgatactg ctactgtact cgctatccac
    11881 aaaagtggat gattagctaa ttttaaataa tccatttata aaacctccct ataaatcctc
    11941 aaattttatt aatatttcta atatatctat ttccaaacct gttttagtac tctaagtata
    12001 tttccaccta gtaccttctc tatttcctca tcactatatc catgctttac taaatatcta
    12061 acaatgtttt ttgaagtttc tgttgggttc tcaagacctt ttacatattc tactttggga
    12121 tgttcttttg ttcctgttat tttttttata tcaaatttag cagctagtac tgagtgtaat
    12181 tttacatggt cgccatatac tgtatctggt ccaaaagcta catggtctat tcctactaaa
    12241 tttttaatat actcaaaatg ttccataaat gactctattg aatgttcttt attcttctct
    12301 gttattgtag tatgaggtgc tgcttctaca cctataaccc ctcctttttt agcacatgct
    12361 attaatactt catctggagt tagtctcttt gtgttccata aacttcttgc cccaacatgg
    12421 cttaatatta ttgggtcctt gctatattct attacatcaa gtgtagtttg aactccaacg
    12481 tgagagcaat ctattgccat tccaatttta ttcattcttt caacagcttt ttttccgaaa
    12541 tttgtaagac ctccatcttt ctcttctttt agtcctgaac ctaatgcatt ggattcgcta
    12601 tatgttatac ccattaacct tataccaaat ccatacagta tatctattct atcaagttca
    12661 ttttctattg gtgctgcacc ttctaatgtt ggtatcaatg ctactttacc ttcactttta
    12721 gctctgtata tatcttctac tttttcgcac tttataacaa aatcttgatg agctaaatca
    12781 catagtctca tgcctaaatc tattaatata tcatcccatt tccaccctga atttgatgtt
    12841 atcgtacata caccatccat taaattatca aatatagcat cataatatcc tcttgataat
    12901 gcttcataag cacatctttg tttaccttcc ctattatatt caaatatatc tctttctagg
    12961 tgttctggaa ataatactgg atgttcatgt aatgatataa atattttttc atccactata
    13021 cttttaacct tagcttcttg aatatcatct aattcaataa ggtattcttt aactctttta
    13081 tctcctttac acatctcaat agatgaaata tcttttcctt tttctaaata actataggct
    13141 tcatatcctt tgtaagctga cttcataaca acatcctcca agttctacaa ttttttatat
    13201 ttttcctatg tagcaattga tgtgccaaaa tataaatttt aattgtataa taaatcttgc
    13261 aaaaaataaa aacagtagta aggttgatat ttactactgt tttattaaaa ataatcttct
    13321 tagattattt attcaatatt tttgaattat cttaatctta aagataattt tatttatttc
    13381 tatccattat agggattttt ttagggtgtt ttgccttttt agtcccctat atcactccct
    13441 taatttttac atatgatata aagttctttc cttcatcagt tataaaacta ccttgttttt
    13501 ttactccaga taaaagtaaa ccatggctct ttaacttatc tattcttttt ctaatttggt
    13561 ctacacttaa aactatatct ttttctttta aaatctcttg cagtttattt cttcctaata
    13621 atatattttt attattccaa gtttctatac tggtcaatat acatatactt tcctcaaaga
    13681 aattagattg tttgaaatct aagataattg aatcgaaatt ttcattaact aaagtattgt
    13741 ttttttcaaa tctaaattgt tctggcaaat gctcgtaatc tactctatct tcctcaacta
    13801 tagtatcaat ataataaatt aaatttttaa gctctcttac atttcctggc cattcataca
    13861 atgttaaagc ttgcattgat tcctctgtaa aacatttgtt gctatttatt tcatctaaaa
    13921 aatacttact tataagaggt atatcttcct tacgttctct aagtctaggt atctctatat
    13981 gtaatacatt aatcctataa tacaaatctt ctctaaaaga accttcttta atttttttct
    14041 ttaaatcttt atttgttgca gctataattc taacatctat tggtataatt ttacttccgc
    14101 ccattctcat aacttctttt tcctgtaata ctcttagcaa tctctgctgt acatctaaag
    14161 atatatcacc tatctcatct aaaaaaattg ttcctgtatg tgctctttca aatataccca
    14221 tttttccacc ttttattgct cctgtaaaac ttccttcatc atagccaaaa agttcacttt
    14281 caataagtgt atctgacaaa gaagatagat taactgcaac aaatggcttg ttttttctta
    14341 gagattcatt atgtattgcc tgggcaaata tttcttttcc tgttccattt tctccaagta
    14401 tcaacacaga aaagtctgta gttgctattt ttcttgcaat attaattttt tcttttatta
    14461 ttttactctc acctacaaca ctctcaaatg tatactttga tacaaaacct tttgactgaa
    14521 gtttattttg aatcttgtcc tcaagcactt gaattgcact tatattttta atgcttataa
    14581 tactctttat tcgttcatta ttttcataaa catttacttt attaattatc agttttttgt
    14641 tatttaaatt tactacttca tcattaactt catcttcttg aaaaaatatt tttttgacaa
    14701 ccttatcact aaataagtcc ataaagttat tagatataat ttcattttgg tctattccaa
    14761 ctagattaga aaatactttg ttgcaatata taaattttcc taatttatca acagaagcaa
    14821 taccttcatc tataatctcc aaaaaactct tcatggtttt attcatagtt gtataatacc
    14881 tatatccact tactgtatct tcattgtatt tttccttaat aatatgtagt ttatctatag
    14941 atatatttag ttttgtaaag atttctataa ctgtatttat atcaattacc ttatcaccta
    15001 tgtcaattat ttgctttatg ttttgtggta tgctgtttct gcttcccgta ataattccaa
    15061 tctcacattt agatttatca caacctggat aatatggtat tagattaata tgaattaatc
    15121 ctagttttct aattatcttg gcagtttcat ctgctgattc tttataagca tctataacca
    15181 taacatcaga atcattttct atacttatta tctgatttat attttcaata tttatagtac
    15241 gatgtactat tacaataggt atatttttat caatattatt atgtatatat tcctttatat
    15301 cattgtctga acatactaca agctcatagc ttgacagttc aatatttatc ctatttacat
    15361 ataccaagtt atctatatta caaaattctc caaatatatt ttccaattgt tttgatatat
    15421 ctatatttat tttgtcatca gttgatacaa ataaaatatt atgttttttc atagtcccac
    15481 ctcctgttta acgagtatag aaagtaataa tttggtctat tgtagcactt tttaaaaaag
    15541 ttttaaatct taaaaatcaa attttgtacc atttactatt attttccata ataaaataat
    15601 gtaagctatc ttttttcaca tataatttaa tttctaaaac ggttagtttg acattattgg
    15661 aatataaaca aaaatttaat ataaattcgt taatatatta atacattttt aagaatgaca
    15721 aggaataaat tataacaaat tttaggaata tataataata gggtctcaga acttagttac
    15781 aagttctaaa accctatttg tttagtgtaa aaaacatcat aaaatttagc ttaaatcttt
    15841 tcacataata ttactctttt tctaagtgat aataaaattt atctattcct ctaaaaactg
    15901 taaaaatttc tgattaaata gtttaagctc atcatagaat atagcatgac cactatactc
    15961 aaaagtatat aaaattgaat cttctatttt ttcattcata aaaactgcaa attcatacgg
    16021 acatatttca tctaatttcc catgaaatat accagtaggc actcttacac attttaaatc
    16081 attaaataat tcttcatctc ttaaagatac agcagttcca atagtgccaa tacctgatgc
    16141 actaaaacca atatcattaa accatcttct aaagctttct ggtggatttg atgcaaatac
    16201 tttttcgcca aaatcggtta ccatttctgg tctatcagta catgcttgtg ctatcaattt
    16261 atttacatct tcacgtgtca taccatatgg aaattctgga ggtctttgta caaatgatgg
    16321 tgcagcagct gcagctaata ccaattttga cactccatat ccattaaata aactcatata
    16381 cctaagtacg attgctcctc ccattgaaaa accaacaagg gtaaagtctt ttaatccaat
    16441 agcatgtacc accttataaa tatcgtctgc taattggcta taagtatatc ctcctgatgt
    16501 tgcatcagat ttaccaaaac ctcttaaatc aattgaaact gttctatatc cgagttttgg
    16561 caatatattt gtttgatatt caaatatctt atgtcctaag ggccaaccat gtataaaaag
    16621 tacagttttt ttagcactcg gattcaaatc atatactgct atgtttatat cattcacata
    16681 tacataatac atatcaacag caactccctt caagaaaatg tatgatggtt attttatata
    16741 tatgaataaa atgatgaata actaaatttt caaatttatt tacaatactt ctctattgcc
    16801 ttttcaaata aacttacatg aagtttttca tctaggatta ttctatttaa tatagctttt
    16861 atataagggt cttcaataag acttatatct ttataataat tattaatagc cacatgctcc
    16921 aaatgcaaat ctagttttaa tctgttacaa atagaatttc cataacatac aaaaccacca
    16981 ttccaatatg caccacaaga acttgtagaa cctctatata caggatttcc acccaataat
    17041 cttatagtct ttgctaatat ctccatatgt aacatttctg ttatagatat atttatccac
    17101 atttcactta actctctatg cgtttcatct aaatcaaaat tatggtatag atattgattt
    17161 acactcgtaa attcacttga tactccggca tagtcatcca tcaataattc tgcataatac
    17221 ttatttggac ctaaaacttt tatctctgga tatggctcat cacttgaata tccatttcgc
    17281 ttgtgcatgt cataatgtgc atcactcata atatctcctc ctttaaattt aatacatgat
    17341 attaattgta tgcatcacga ctttttcata taacttagat aataaaatct acttaaataa
    17401 taaaaagcca tattaattaa aattaacatg accttttatc cacaaaatta ctttttaatt
    17461 attcatcttt tccagctact gatagctctt taataacaac tgaaggtgaa cctatactac
    17521 tcattggaaa ttctaaatct tcccctatag cttcaatatc tttcaacagg tcaaaatagt
    17581 ttcctgctac tgtaatttgc tctactggaa atactttttt tccttctttg atataaaagc
    17641 ctttggcagc taaagaaaag tctcctgtta ctgaatttgc acctgaatga agtcctgcaa
    17701 agctagtaac catcaatcct tcatctatat cctttataat ttcatctaaa gatttatctc
    17761 ctttctctat ataaaaatta gttggatata tgcttatagg tgatgaatat gaagatttta
    17821 ttccatttcc tgtagtcttt acacctgcct tattagccgt ttttaagtta tgaagtaaag
    17881 ttataagctt tccatttaaa acaacttctt ttttaaatgt agctactcct tcatcatcaa
    17941 aaggagtaga tgccatacca ttttctaaaa gtgggtcatc aactattgta actataggag
    18001 atgctatcat gtctccttct ctatttttca ataaagataa tcctttttga gctgaatctg
    18061 cattaaatat cccagaaaat gtgctaagca aagataccat agcttcatta agcaatatag
    18121 ttttatattt acctgatgga atactcttac cacctacttt agataaagca ttatcaacac
    18181 caagttttgc tatctcacaa ggttttattt cttcaataga atttgctata ttatatccta
    18241 ttccatcata tttttgtcca ttgtcttcta tgataggaac tacaaaccct ataagcatat
    18301 tagttttatt gcttaaatta agacctttag tattagatat accattactt gatacagaat
    18361 aagatatagt acatttagat aaatttacca ccttatctga ataagcttta gtctctcttt
    18421 ccatttctaa agctaaatca atcaacttat ctgcctctaa attttctagc tctttagaat
    18481 aagtctttac atcattatag tgcttatctc cttcatatat aaattgtaca tcttcatttt
    18541 caatagtgtt tactccatct tttacatttt ttatcaacat atcaacagct ttatcatcta
    18601 aaatttctgt ataagaataa cctatctttc catttacctt acctctaaaa gataaaccaa
    18661 aagatttatc aagattgtat ttctcaactt ctccttcata tatatttata cttaaatttt
    18721 ctcctgtata ataatatatt tcatactctt caaaaccttc acttagagcc tttttaaata
    18781 atatatcttt aaaattttta agttccacaa ttatccctcc tatcttccac ctacagtaat
    18841 ttcttttacc cttatcatag gttggcctac attagttggt atacttcctg atgatgagcc
    18901 acacatcccc tgagcttgtt ttacgttatt cccaaccata tctatattca taagtatatc
    18961 gctacctttt ccaattaaac tagcacctct aactggctct tgtatttctc cattttttat
    19021 taagtaacct tctgaaactg caaaattaaa ctctcctgtt acaggattta cagaaccacc
    19081 acccattttt ttagcataaa gtccatctgg tattgacttt ataatatctt ctggtctatc
    19141 ttctccagca gcaatataag tgttagtcat ccttgaagtt ggtgcaaatt tgtagttctg
    19201 tctacgagaa cttcctgtag gattcattcc cattcgtcta ccattaagtt tatctatcat
    19261 ataagacttc aaaattccat tttcaatcag tatattcttt tgagttggat ttccttcatc
    19321 atctatattc atagaacccc agtagtttgg tattgtacca tcatctatag ctgtaatctt
    19381 tgtggaagca atctgttgac ctaatttatc tgcaaataca gagttcccct ttgcaactga
    19441 tgtagcttcc aaagaatgtc cacaagcttc atgaaatatt actccaccaa atccattatc
    19501 tattgctacc atcatattac ctgcaggaca atttttagca tgcaacatag tatgcgctac
    19561 tctagctgat tcttttgcat aatattctgg gtctatttct tcaaatattt caaacccttt
    19621 acaaccccca ggaccttcaa atccagtttg attttcatta cccttagatg caattgaact
    19681 tatccctagt ctggttctta ttcttctatc ctcaacatat ataccttcag tgtttgcaat
    19741 taatattttt tggtctttat ccaaatagct aacacttact tgagatatat cattactaaa
    19801 ttcttttgca ctcttatatg caaccttcat aacacttact ttatctttat ttcctattga
    19861 atttggataa atttttatat tatgtatgtt atttatttta atactgtcat ttaatattac
    19921 actttttcca tcttccaatt tacctaaagc aacagcagct ttgtatgcta catccaacag
    19981 actatttaat gaattgtcac ttgtataagc atatatactt ttcaatcctt taaatattct
    20041 tattcctata ccataatttc ttccacctat tgcattttct actttattat ctattaaact
    20101 aatagaatta tttatagtgt cttcttcaaa aatctcagca aaatctcctc cagtaattag
    20161 acatttttct aataccttag aagctatatt ttttgaaagc ataaatttac ctcctacttt
    20221 aaatttttaa ttaactttta ttcaatcttt ttacttgtta acaaacatta tatactatat
    20281 tacttaaaat aaggtaatta tgaatatatt atacatatgt tttatactaa aatgttatca
    20341 cattttatct atacataccc atataactag ataattaaaa tgtataatat tatttgctat
    20401 agaaaataat acactcatac ataataaatt ttaggagtga agtatatgaa tcaagaatat
    20461 gtaaaaggaa atcctatttt taccaatatt aataaagttc caagacaatt tccatacctt
    20521 actgatgata ttgatactga tgttataatt gttggtggtg gcgtaactgg atgtatttgt
    20581 gcctactatc tagctaaaaa caatataaag tctgtcattc ttgaaaaagg aaggattgca
    20641 catggaagca ctagcgtgac aacttctcta ttacagtatg aattagatga caatttgata
    20701 gatttaacag aggttatgac tctaaaagat gctctaaaag catataacct ttgtacttca
    20761 gcacttgaag aattagatac atttatagag ctatatggaa ataagtgtga ttatgccaaa
    20821 agagatactc tactttatac tgccaacaaa cttgaagtta aggctatcaa agaagaatat
    20881 aacttaagaa aagaaaatgg atttgatgtt gaatatattg atgaatccac aaaccctttt
    20941 aactttgatt taaaatcagg attgatagca aagaatggag gacgagaatt agacccatat
    21001 aaatatagtc accatttgat tgatgtaagt ttaaaaaatg gtcttcaagt atatgaaaat
    21061 acagaagtaa aaaaagtaga tttatcaaat gataaggtta ctgctgaagt atcgtatgga
    21121 tacaaagttc atggtaaaaa attaattgta gctacagggt acaatacaag tttatttaca
    21181 aaaagaaatt ttgcaactaa gtcaaatact tttaatatag caacgaaacc acttaaaaac
    21241 atagcttctt ggaaaaacaa cattttaatt agagataact gtgacccata taattatcta
    21301 agaactacta aagacaatcg attaattata ggtggtgaag atgtaagttt tgatgatata
    21361 gaaaatgaaa ctttagccaa tgaaaagtat gatattttag aacaaagatt gaaaagtatg
    21421 tttaaggata taaaagacat tgaagttgaa tataaatatt gtggttgttt tgcttcaact
    21481 ttagataatt taggttttat tggtccagat aataaaaata acaatctatg gtattgctta
    21541 ggttatggag caaatggtat actctttgca atattaggag gtataatgtt aagtgaatta
    21601 tatcttggca aacaaaataa aaatatgaaa ttatttaaag tagatagatt tgataaatag
    21661 atattatata ttaaaagact gtaaaaagat gaaactttat aattctaaaa gttaactttg
    21721 atacagtctt ttataatgtt ccttttaaaa atactttatt tataattaaa tataaagtaa
    21781 aatgttaaaa tataactgat aaaatttagt cataagataa tatttaagtt tatttgatga
    21841 aatatggagg aattattatg aaggaaatac tcgttattgc tcccactaaa ggaacctatg
    21901 aaaaatcaat tcacatagtt aaaaaaaaca aatatacaaa tattgatgtt gtatttggca
    21961 atctaaaaga aggtatacct cttgctgaaa aaagtattaa tcatggtact agaattatca
    22021 taagccgagg tggaacttac aatatgttaa aagctactta caatatacct atagttgaaa
    22081 ttaaggtaga tgcttatgat attataaaaa gttacaaaga ggtaaaaaat tccaacgaac
    22141 catttggaat aattggattt aataatgtca tctacggttt cgatattata gaagaaatat
    22201 tgaataagaa aataactatg attgaaattg aaaaagaaga agaaatttat gatgctatag
    22261 aaaagtacag aaagaaaggt ataaacacat atataggaga taccactgtt gcacatattg
    22321 ttaagagact taattgtaag ggaatattaa tcgaatctag agaagaaaac atacttagag
    22381 ccattcaaca agcagaacaa atactcgaag caaccaagga tgaacaaaaa agaagattac
    22441 agatagaagc tatgacagat tttgtacatg atggaataat aacagttgat aaaaatttta
    22501 aaataacatt atttaataag agtgctgaaa aaatatttgg tataaaaaaa gaaaatgcac
    22561 tttatcataa tgttgtagat gttatcccaa acacagttct tccacaagta gttgagacta
    22621 tggaacctca aattggtgaa atacaaaata tagggagtac aaaaattatt acaaatagag
    22681 ttccaatcat tgttgatggt cagattcaag gggctgtttc aacatttcaa gatagcaagg
    22741 agataagttc ttttgaacat acaataagaa ggagcttatt aagcaaaggt ctatctgcta
    22801 aatacacttt tgatgatata attcatgcta gtgaaaagac aaaagattgt atagaacttg
    22861 caaaaaaata tgcttgttat gatactccta tgattattac tggagaatct ggagttggta
    22921 aagaactatt ttgtcaaagt atacataact atagtaagcg taaaaatgca ccttttgttg
    22981 ctgtaaattg tgctgccata cctccatctc taatagagag tgagtttttt ggttatgagg
    23041 aaggttcatt tacaggtgct agaaaaaaag gtaaagcagg tgtattcgaa ctagcacatg
    23101 aaggtacaat cttcctagat gaaataagtg aaatccctct tgaacttcaa ggtcgattat
    23161 tgagggtatt gcaggaaaaa caagttatga gaattggtgg tgataaagtt attcctatag
    23221 atgtaaaaat tatctgtgct tcaaataaag acttaaaaaa tatgatgcgc aatggtttat
    23281 ttagacaaga cctttatttt agaataaata tactaacatt atatcttcct tcattaaaag
    23341 aaagaaaaga agatattata aaattatcag aattttttat acataaatat tcaagcaaat
    23401 acaataaaac gcctttaatt atcactccaa atattaaaaa ggcacttctt gaacgagaat
    23461 atgaaggtaa tataagggaa ttggaaggta tgatggaaag ggctgttatt gtggaatcat
    23521 ttgactcgat tttattagaa aaggttagca atgaatatac aaataatata gttgataaag
    23581 ataatagtga ttttgaatgc agtaatttag atttaaaaac tttagaaaaa aaatatatct
    23641 ataaaattta taacgaaaat aatagaaata ctacaaaaac ctgtgaaatt ctaaagataa
    23701 ctcgctccac tctatggcgt aagttaaaag aaatagaaga tgatgtttaa aaataataca
    23761 tacctatata tatcaaaacg ttttatgttt aattttaata cataagacgt ttatttgttt
    23821 tcaataaaaa atacaattat atgataaaaa gaatctttgt aagtgaaatt ctcgtcaatc
    23881 gtacttttaa ttataagtaa tctacagcat ttcaatcgat tattaaaaat aatcatgata
    23941 actaaagata caaactctat atattggcat attaattgct tttataatac ctatataaaa
    24001 atgaaaggga gtgatactag atgcacatct taaaatcagt gaaaaagata ccaggaggac
    24061 ttatggtctg tcccttgtta ttgggagcac tatttaatac agtatgtcca caagctcttg
    24121 aaattggagg attcacaaca tccttattta aaactggtgc aatgagtata cttgctctat
    24181 tttgtctatg caatggagct caaatcaata tcagacaagc tggtaaacca ttgactaaag
    24241 gtatagtact tacagcaaca aaatttataa ttggtgcagt attaggtatt attgttagta
    24301 aatttattgg acctaaaggt ataattggta ttacacctct tgccatagtt gctaccatga
    24361 ctaattcaaa tggaggtcta tttgctgctc ttgcaggtga atatggagac tccacagatg
    24421 ttggagcaat atctatctta tctctaaatg atggtccttt ttttacaatg cttgcttttg
    24481 gagtaactgg acttgcaaat gtacctataa tagcactatt tgcagcactt ataccaatac
    24541 tacttggatt tatacttggt aaccttgatg aagatatacg agaatttctt gcacctggta
    24601 caacattatt aattcctttc ttcgcttttc ctttaggagc agcattaaac tttaatcaaa
    24661 ttataactgc tggactacct ggagttgtac ttggtttagg agttactata ataacaggta
    24721 tgggtgggta ctttgtaatg aaacttatac gtgcagaaca tccagcagta ggtgcagcta
    24781 caggtacaac agccggaaat gcagctggta ctcctgaagc tttagcagct atagacccta
    24841 ctctagctac aatagcagct gtttcaacag tacaggttgc tgctgctatt attgttacag
    24901 caatatgttg tccaatgtta gtttcctatc tagataaaag agaaaaaaag aaaaaatcta
    24961 tttttaaaac aaataaaaat attgaagtaa attaatactt aaggaggtaa aattaatgaa
    25021 tgcaattatt catgcaaatg aaaaagataa tctagttaca tgtgtcagac ctttagttaa
    25081 aggtgaaaaa gtttcaattg atggaaaatc ttacatagta aatgataata taccagtctt
    25141 tcataagatg gctacagaag atgtaaaaaa aggtgatgtt gtatacaaat acggtgaaat
    25201 tataggaatt gcaacagttg acataaaaac tggtgactat gttcatgtac ataacattga
    25261 aagtacacgt ggtcgtggag ataaaaaata ggaggaagtt aaaatgaaat taatgggtta
    25321 tttaagagaa aatggtcagt ttggtattcg taatcatgtt ttaattattc caacatctgt
    25381 atgttcaagt gagacagcta caagaatagc atcacttgtg cctggagcaa tagcaatccc
    25441 tcatcaacat ggatgttgtc aaattggttc tgatatagag cttacagcaa agacattaat
    25501 aggatttgga aaaaatccta atgttgccgc agttttagta gttggtcttg gatgtgatgg
    25561 tattcaagca aaagaattag catctgaaat agctacaaca ggaaaaaagg tagattacgt
    25621 tgttatacag gaatgtggcg gaacattaaa aacagtttct aagggagctg aaatagtaag
    25681 taaaatggct cgtgaagtat caaaagaagt aagagttgaa tttggtatga gtgaaattac
    25741 attagcactt gaatgtggtg gttctgaccc aacaagtgga atagcttcaa acccttcaat
    25801 aggcgttgca tcaaaccttc ttgtagatga aggtggaagt agcatactaa gtgaaactac
    25861 agaagtaatt ggtgcagaac atttgttggc tacccgtttt gaagatgaag aaatgaaaga
    25921 taagtttctt aaatttgtaa gtgatgtaga aaaaagagca attgctatgg gagaagattt
    25981 acgtagtggt caacctactc caggcaataa aactggtgga ctttcaacaa ttgaagaaaa
    26041 gtcacttggg tgtatgtata aggcaggcaa taagccattc aaaggtgctc ttgaatatgc
    26101 tgatattgta cctcctgata aaaaaggatt atattttatg gatactccag gtcaagatat
    26161 agattctatc actggtatgg tagcaggtgg tgcacaaatt gtaatctttt caactggtag
    26221 aggtactcca acaggaagcc ctatttcacc agttattaaa atcactggga atagtgatac
    26281 atacaacaaa atgccagaca atatcgatat aaatgcaggt agaattataa cagatggtgc
    26341 taaaatagca gatataggtc aagaaatatt taatgaaata atagaagtct gtaatggtaa
    26401 acataccaaa gctgaaagtc ttggtcatag agaatttggc atttacagaa tttcatctac
    26461 attttagatt gtaacaaaat atcttttata aatataatac ttatgtagat acataagcta
    26521 aaagttcttt ctaatactat aaaaattttc tatactgaaa aggaactgta tcaaaattat
    26581 tttgaaaaca gttctttttt taacctcttt tactcaaaat attataaaat aagatttaaa
    26641 caataaacgt atcttgatta atctctaaga agacataaat aaagctaaat gtaaagtggg
    26701 ataaataaaa tttaatatct ttgtatgaaa agatatttgg tataatatgt ctgagagaat
    26761 tcttatttgt aaacaaataa aggatggagg atgaaacaaa agaatgaact ttgaaaacac
    26821 aaaaaataaa aaaataatag ctactttact attgacactt cttttaatcg tgtcattaac
    26881 atgtgtcact tttattatga atctaaataa agtaacacat aatgaaacag aacgatatct
    26941 tgtagaagtt tcacaaggta tttccactac aattaatgtg aaatgtaaaa gtatatttag
    27001 gacactatat tctatgggag atacttattt tgagattaat gatagtgaaa aagaagtcaa
    27061 tagatactta tcaaaaaaat taaaattatt tgaatttgat tggttaggta ttatagattc
    27121 taatggaaat tttgtaagtg cccttaaaga tacaggtaat gttaaaggac aagactttta
    27181 tagaaaagcc cttcaaggca aagaaacaat tactcctgtt aaatcacaat ctggtacata
    27241 tggaatttta tattctgtac cactatacaa tggcgataaa attgttggag ctgttgctgc
    27301 ttggaatact attgatacta tgagaaattt attaagtgta gaaagctttc atggtgaagg
    27361 attttcccaa attatcgacc aagatggaaa ttttattgta agttcagcaa acaaaaatgc
    27421 tccaaaaaat gtggtaaatt tttttgacat gattaaacac caaggaaaac aggttgataa
    27481 aaataaactt gaagaaatga aaataaactt aaaaaagaac aaaactggtt ctatgtatta
    27541 tacattaaat gatggtattt ctaaggcaat gcactatctt ccattacaag aagaaaactt
    27601 atacctcctt tcaattgttc caacaaaaac agcaagtgca cagtttgatt tacttctaaa
    27661 aaaagcaatc ttcatcaata ttttaattgt tatactattt ttattgctta ttatattgat
    27721 tatgtttata aatcaaaaaa acagggcaca acttataaaa atcgcctatg tagactctgt
    27781 aactaaaggt tacagtaaag cacgttttga tattgaagct aaaaaaatta tacagtcatc
    27841 ttcatcagaa agctattcat tggtatctat aaatattcag aaattcaaac ttattaatga
    27901 ttcttttggt agtgaagcag gagataaaac attaaagtac atacatgatg tcattagtaa
    27961 ttatctagaa ccaggagaat tgctttgtcg agtttctaat gaccagttta atgtacttat
    28021 taaaactatt cctcaaaaag aaattcttaa gtacatagaa aatatcacca atgatattaa
    28081 tagtttcaat aaaaatgaag aatgtaaata ttttatagct ctatctatag gtgtatataa
    28141 aattgatgac ccaaatctac ctttagtttc aattcaagac cgtgcaaatg ttgcacgaaa
    28201 aaataataaa ggaaacattg gaaatcatcc actttataca tgtgtttttt atagtgattt
    28261 agagcgttta aaaatgatta aagaaaaaga aatggaaaat agaatggagg aagcattaaa
    28321 taataacgaa tttatagtat atcttcagcc taaaataagc ctcaaagata atactattgt
    28381 aggtgcagaa gcattagtaa gatggcaaga cccaaataaa ggccttatac caccagacga
    28441 gtttattcct ttctttgaaa aaaatggttt tattacaaaa ctcgaccact atgtttttga
    28501 aaaagtatgt agtatgataa gaaaatggat agataatgga gaaactccaa tacctgtttc
    28561 agttaatttt tcacgtgcta atttaaataa tatagatttt ttagaaaaat acagaatgct
    28621 tcgtaataaa tacaacattc ctgctaagct cttagaaatt gaattaactg aaacacttgt
    28681 atttgaaaat ctccaaaaat taattaatat aatagaccaa atccatgaag aaggttttca
    28741 atgttcacta gatgactttg gaagtggata ctcttcactc aatatgttaa aagaaataaa
    28801 agttgacaca ttaaaacttg accgtgcttt ttttagctca cctaatgcag ataatattgc
    28861 tgaaaatcat gtaatagaat ctatagttaa tttggcaaaa aaattaaata tgaatagtat
    28921 atcagaaggt gttgaaacaa tcttgcaaat ggattatctt aaaaaaatta attgtgatat
    28981 gatacaagga tatgtttttt caaaacctct acctcaagaa atatttgaga aaatgacttt
    29041 tggaaaaaaa ctagaagaaa ccagtatgga tagttccgtt taaataaaat aaaaaacttg
    29101 tctctataaa ataattttaa tcattttata aagacaagtc tttttatttt ccaatttaat
    29161 tttattttct aaatttctta cactcggctt gttttaaatt ccctttttaa gaataataat
    29221 acgattatca tagcaataaa atctgcaact ggctgtgcca accaaacacc atctaaatta
    29281 aattgaattg gaagtacaat gattaatggt ataaataaaa taaattgtct taaaagagtt
    29341 aaaaacattg aatgtttaac tttacttata cactgaaaat acacaggtcc tacaatagaa
    29401 atccccatta taggcaatgt aattagattt aggcttatac ctcttactgc tatatccatc
    29461 aaatctgaat ctttattaaa tatacctaca aatacttctg ggaaaacttg tactactaaa
    29521 aatccaaatg acaatatgac aactgacact atgatagata atattaaagt tttctttgca
    29581 cgctcatatt gttttgcacc ataattgtat gatattatag tttgcatacc ttgattcaat
    29641 ccaaacacag gcatcataaa catcaataaa atagatgtaa gtgctgtcat agctccaata
    29701 gctaaatctc caccataaac ctttagagaa ttatttgtaa acatgtgagt tatacttata
    29761 gctacttcca tagcaaaagg tgtcattcct attgcaaata ccttctttac tatatcttta
    29821 ttcaatttta agttacattt ttttagtttt aaattggact tccctcttat aaaataatgt
    29881 attacccaaa aaaatactac tatttgacag agtactgttg ctatagctgc tccttttata
    29941 cctaaattaa atacaaatat aaatataggg tccaatatca ggtttaatac acatcctaca
    30001 atcattgtcc ttgctgctaa ttttggatta ccttctgccc taattgcatt attaagagaa
    30061 aatgcaacta aattaaatat acttccaacc aatatgacac tcatatagtc ttttgcataa
    30121 aatattgaat ctttacttgc acccaaaata aaaagtatct cttctaagaa aactaatcct
    30181 aatatagtta taattaaaga gataattata gataatgtca aagcattacc taatatcttc
    30241 tctgcttctt ctctatttct ttcccctaac tttatagaca atcttgtgct agcacctact
    30301 gataccaaca ttccaaaagc tattattaga gtaaatactg gcatagttac accaagacct
    30361 gcaatagcta tagagccaat accttccata gaaccaataa aagctctatc tactacatta
    30421 taaagtgaag ttaccatcat tgctattatt gcaggtatcg aatacctaaa taataaaatc
    30481 catatttttt catgtcttaa tgcttcttga ttctccatgt tgtcaccatt tacaccatgt
    30541 cataagttat acaaattggt ttatttgttc ttgggtctgt tgctatttct gcatcaatat
    30601 taaataattc ttttagatta tccttcgtca taacatcata tgcatcacct tcacaaacta
    30661 ttttaccttt cttaatacct atcatgtggt cagcaaatct tgctgcatta ttaagttcat
    30721 gtattaccat gacaatagtt cttccttgtt ttttatttag ttcatctaat aattttagta
    30781 tctccaattg atgtgccaaa tctaaatatg ttgtaggttc atccagtaat aatatatcag
    30841 tttcttgtgc caaagccata gctatccatg ccctttgtct ttgtcctcct gataaatctt
    30901 ccatatttct atccttaaat tctgcaattc cagtaacttc taaactccaa tttacaatct
    30961 ctctgtcttc tttactagaa ttaccaaatc cacttttatg aggaaatctt ccataagaaa
    31021 ttaattctga aacggtaaga ccactaggag cttgagggct ttgtggaagt acagccatat
    31081 cttttgctat ttctttagac ttctttttgt agatgtcctc tccatttata tatattttcc
    31141 cactattagg gctaataatt ctacctatag ttttaagtat tgtcgatttt ccacaaccat
    31201 tagcccctat gatggtagtt atttttcctt ttggtatatt taaatttaaa ttttttataa
    31261 tatctgtgtt tccatatgat acatttagat tcttagtatt tatacagttc ataacaacac
    31321 cctttcctaa tcatttacca acattaaata aataaagtac ggcactccta ttatagaaac
    31381 tactatacct actggtattt ctataggtgc tagaagattt cttgatatag tatctgctat
    31441 taaaagtaat aatgttccta tcaatgctga tactggtatt aatcttttat gctttggtcc
    31501 tattattttt ctacctatat gtggagctat taaacctaaa aatgaaatac tacctgctac
    31561 tgctgtcgat acaccagata aaattactgc atatattata agtcttcttc tttgtttttc
    31621 tacatctaca ccaagactta tagatacttc atcacccaaa tttaaaacat ctatatattt
    31681 agatttataa atagttatac ccataaaaat cactataaat ggaagcacag ctattacata
    31741 tttccaactt gttccccaaa tgcttcctga tgtccatgcc ataaccctat taaattcttg
    31801 tgttgtaaat ttcaattgga atatcgtcaa aattgaagta aaagctatgt ttacacctat
    31861 accaataagt agtaatcttg acgaatttat accacctttc catgcaagag aatatataag
    31921 aaatgctcca aataaagctc ctataagtgc aacaataggc attgtaaata cagtcaagtt
    31981 actcatacct tcatatacat taccattcat aaggtatata taaactacta caaacaatgc
    32041 agcacctgaa tttattccca aaattccaga atcagctaaa tcattctttg taactccttg
    32101 aagtattaca cctgctgttg ataatgcaga accaaccaaa agtgctatta ctattcttgg
    32161 taatcttaat ttaaatattg caatttcatg attacgctga ccctgcccta aaaaagtctt
    32221 tataacatcc attggttcta tagaaaatga gcccatattt aaactcaata aaaaagtgat
    32281 aaaaataagc accattaaaa ttgatataac aagtaatgtt ttagaattta tttttataat
    32341 atttttcata gtacctattt ctcccccttt ctaacaaggt atataaatat tggcactcct
    32401 ataagtgctg ttatagaacc tactggtgtc tcatatggag gatttatcat cctacttaat
    32461 atatctgtat atacaagcaa tacagctcct aatactaaag agctcggtat tatatactta
    32521 taatctgaac ctactattcc tttagctatt tgaggaacta taagacctat aaaaataata
    32581 ttaccagaca ctgacacaga tgctccagtc attaatatta caagtactat acatataaat
    32641 ctaacaaact tagttttttg tcctaatcct attgccactt cttctccaag acttaatata
    32701 gttattcttg gagccataat aatagctaaa ataaatccaa taccaccagc tacaaatagc
    32761 aattttatac cttcccactt cactcctaca agccctcctg atatccagaa acttaattgt
    32821 tgagataggt taaaatacat agatattgct gatgccaaag aaattagcaa tgtaccaaga
    32881 gcgactcctg caagagctaa ttttacatta ttaactcttc ctcttgattt agaacttata
    32941 aaatatataa ataatccact tatacttgct ccaataaatg caaacatcat tacactaaaa
    33001 cttccatgta taagttgagg aagttttttt tgaagtacca gtgatatcga aattgcgaat
    33061 gtagctcctt gtgtaattcc tataacagat ggttcagcta taggatttct cgttattcct
    33121 tgcattattg ccccagaaac tgctaaaaac cctccaacaa gtgcagctgc tattgccctt
    33181 ggaagtctaa catcctttac tattctcata tttattccat cttccatttt tatcaaacta
    33241 tctataacag ttgatagatt catatctttt gccccaatag ttatggataa tacaattcca
    33301 aatacaagta caactatact tactaatatc aacaaataag ttatattttt tttactcagc
    33361 ttagtcaatt atcctacctc cttttccctt ttaatcgtca gtataatatc atctattaag
    33421 tattttaatt ttaagattta ttaaaaatag gtgtgaaaaa tgaatatcat tatctcacac
    33481 ctactaagtt aagaattact ttgttaattc atttttaact gattctaata ataactcttt
    33541 tccaattgga ttgtatgatt gactgaaaaa tggagcagca tcaagtattg ttacattacc
    33601 atcttttact gctcttattt gagcccagat agcactattt tctaaatctt ttttatctgc
    33661 ttctgtagca attacaacta tatggtctgc atctatatct gttaaacctt ccatacttac
    33721 cattggtagt gttattccat cctgttttgg catattctta ggtctagcta atttcatatc
    33781 atcattgatt agagtaccta ttccaccatc actaaatacc ataaattgac ctgaacttgc
    33841 caacactggt aaataagttt tttctccatt cttttctata acttccttac ctaactttgt
    33901 agctttttca tcatacttct gtagccaact tttatcttct tcttctttgt caaataactt
    33961 tgaaacatca gttaacttgc ttctccagtc atttgcataa tctttcatca taactacagg
    34021 agctatttct tttaattggt catatatttt ttcttgtctt tgactcatta ttattaaatc
    34081 aggatttact tctaatatag cttccatatc catagtatcc atcattgagt gcccaactat
    34141 ctttacacct ttaagctctt ctcttatgta acttggtaat ttatcagttt catatgagtc
    34201 tacatttgct gttgcaacag gcttataacc tgctaataat agttcttcac tacttcctga
    34261 tatatcaact atttttttag gatttgatgg tatcttaacc tctcctttaa cagattgtac
    34321 aactcttgtc tctgatttat ctttatcctc tgtattctta tctgaacatg ctgtaacaag
    34381 tactaacgta gttataatag atataaatat tgcaagtgat tttatttttt tcatgattac
    34441 ttctcctact tgtttattat acttacatga tattgattat cataagcaca tatttattat
    34501 agcatttttc tatttttagt caatattatt ttgtaaaagg ataaaaattc ctaattaatc
    34561 ttaattatat atagcttaga taaggttttc ttatattaaa cagtattttt tttaagaatt
    34621 ggacacaaat acaatgactt ttcagttaaa caatgataaa atgcaattta tagataagga
    34681 atttagtagg gaggattcaa aacatggtac atttagtata ttgtgataat gcaggaaaaa
    34741 aaggagaaaa ggtcttagat aaaatcttgg atggaacaaa aacaatggtt attagaggtg
    34801 ctgctggtag aaaaatacca catagtagag tatttgaaga tgagttactt tattttatgg
    34861 aaaaaggaac tgcaaaaata tctgcaaaag ccactgttaa aaaggtagaa aactatgtta
    34921 aattaacaga agatgaaatt tctaaaattt taaatgaaaa tcaaccaaaa ttaaatttat
    34981 ctgaaaagca aaaagttcgt tggcataaaa aatgtttatg cttagttgaa tttgaaaatg
    35041 ttgaagaaat aacacctctt gattttgacc atcaaggaaa tatggatgat tggcttattg
    35101 ttgaaaaaat tgaagatgta gttgttggta ctagtatacc ttacaattat gagaagtcta
    35161 aattctaaag tatcaataac ataatactat attgctaaac tctacttaaa taaaaaaaga
    35221 gtgcatttaa taattataaa aattattttt atacactctt ttttaataat aattttaaat
    35281 tacttacttt ctaatctttc atatacatct ataaattctt ctgctaattg ttgaaaattc
    35341 atagctgtca ttaaatggtc ttgtgcatga ataagtataa cacttatttc agttttatta
    35401 ccattagctt cattttgtat caattctgtt tggaatttat gagctttatg aattgattct
    35461 ttcgactttt caatacattc tctagcttta tctatattcc cgctctttgc ttcttttatt
    35521 gcttcaaatg ctagcccttt cgcatcacct gcaaatccaa ttattccaaa agatatctct
    35581 atcatcttct catccattta ttcgacctcc taaatatctc tatagcttga tatgactaat
    35641 ccattctttt ctaaaaattc tttgactttt ttagatgtaa gtatcttgtg ctctttcgtt
    35701 ctatcaatag cataagatgt agaatttaaa atataatcat ctaaaaaagc tgggtgagac
    35761 ataatatcca ctacatcata cttcattatt tcgtctatat tttttataaa atattcttca
    35821 cttacatttt ccttataaaa actatctatt aaaggaacta ctttagaata ctctaaatta
    35881 tattcaaatc cacctcttat tggaagatta tacttggtta caatcttctc tattactggc
    35941 tttaaactta ctaaagtatg gatatgatgg tgactatcta aatgagatat atctattcca
    36001 agccctttta ccctatctat ttgagcacaa agttctctat aaatctcatc acaatccatt
    36061 ttttcaataa tttcgtctgt aatcctccta ataaaaaaac cgtctttatc aacaatagtt
    36121 tttaagttag ataataatgg tctaccacaa cttaaagtca tatgaactcc acaatttaaa
    36181 gttttatttt ctttaagtaa gcctactcca tgttctaccc caggcatatt tgccatcata
    36241 gaagtagacc taactatacc attattatga gctgaaatta ttccatagtt cacagcttca
    36301 caatatccaa aatcatctgc atttattata attttagtca taccataacc tactttctat
    36361 agacaactat ctattaaatc taggaagata atctttatga gcttccaaca tttcgtctaa
    36421 tatagttttt gctaagtcat cagatggaat caatgggttt atacaaagtg ccaaaagagc
    36481 agattcataa tcgccatata cagctgcttt agctgccaat acttcaaatg atttgatttg
    36541 acttactaat ccatgcacag aatctggtaa atatcctata cttattggtt ttggaccatt
    36601 ttttgttatt acactactta cttctactgc ttgttcatct ttaaaatctc ttatagaacc
    36661 cttatttaat gtattaacta cttggatatc ttttttgtca ttgtatatag aacttattaa
    36721 attacaagca gcatcactat agtaagcccc tcctcttttt tcaagttgag gtggttttac
    36781 atctaatttt tcatccttat ataattcaaa taattgttct tccaattctt taactacttg
    36841 acctctagct tttccctctt taaactctct caactcatcc tcaagcattt ctttagtttt
    36901 atagtagtat ctatggtatg gacaaggtat tgcacccaaa gattttacaa attcagcatt
    36961 aaaatcaatt gcctttatat tttgcatact tatatctgct ttgacaaatt tatcaattgc
    37021 ttcttttgta acatcttctc catctaaaga tacatttaga ccataaacca tatgattaag
    37081 acctgcaaaa tccatactta ttctgtcact ttctacatta aatagtttgg caacatcatt
    37141 ttttaaatga attggcacgt tgcatagacc tatatatctt ctaaaattag tatatttgaa
    37201 tacagcctct gttattattc ctgttggatt tgtaaaattc accatccatg catttggaca
    37261 taattcttcc acatctttta ttatatcaaa tataactggt attgtgcgaa gggctttaaa
    37321 taaacctcca gcaccattag tttcctgccc cataactcca tgagataaag gaatagtttc
    37381 atcttttatt ctcgcatcta aaagacctac tctaagttgt gttgtcacaa agtctgcatc
    37441 tcttagagct tctcttctat ctaaagtaag atttattttc atatctactc cagctttttt
    37501 taccatcctc ttagctagat ttccaactat ctctaatttt tcttttccag cttctatatc
    37561 tacaagccat aattccttta caggtaactc ttcatatcta tttataaaac cctctacaag
    37621 ctcaggagta taacttgaac ctcctccaat tgtaactatt tttagtccct ttttcatctc
    37681 attataccat ccttacctat aatttaatat actctacaaa accccataca tttatttaaa
    37741 actctataca tttatttacc gaaataaaac tacctagttg ttaattttca atttattagc
    37801 ttccattttt tcagacgcta tgacaaatgg aatatatatt aatattgata atattaaatt
    37861 aaatagtgct aaagctccgc cagtccagtc acctgtagcc attgctccac ctaatatagg
    37921 aggtgttacc caaggtattt taggtaaaat tactggtgct actaatccaa aatctattgc
    37981 taagtatgat acagcactac atattactgg tgcaagtaca aatggaatca taaatatagg
    38041 atttaaaact attggaagtc caaatatcaa tggttctcct atgttaaata ttgcaggtgg
    38101 accacctaat actatcatct ctttacgtct tcttccagct attaataaag ctactattaa
    38161 tcccaatacc attccagaac cacctaaata tacaaatgca tcaaagaatg cacctgcaag
    38221 tacatggtat ccttccgttg ctcctgcttg agccaaggca gcattttctc cacctaattt
    38281 cataagtata gtttctgtaa aaggaagtgc tatatttgca ccatgaagtc caacagtcca
    38341 aagtatatga attataaatg caataagcat tgtagaacct agactatcag ctacatttga
    38401 tagtggtgct cctaaataag tatttaaaat atctgttaag aaacttccat tagaaagcat
    38461 ttttataaat attcctatta cagtaaatat cattatagtt aacattcctg gtattaactt
    38521 tgcaaatgat cttgaaactg ctggtggaac tccatctggc atttttatag ttaatttctt
    38581 taccttagat aatcttacaa atatctcagt tgataataat cctattaata tagaagagaa
    38641 taaagctgct gtacctaagt aggtttgttg tatcattccc catccttcaa ctactgtagt
    38701 tcctccctca ggaactattt taccaatttg tggagccatt ataaaaaaga tagataaagc
    38761 tataagccca gattgtaacc catcactctc ataagatttt gctagaaaat aagcaacacc
    38821 tattactaag aatactgcca ttgtacctat tgctccccac caaaggtcgc ctccaaatgt
    38881 ggtccagttt tctccaaata tacttgccat caaatttttg tatgcttcca atggtaagtt
    38941 atttatcaat gttcccaatg cacctaccat agttataggt atcattacta taaatgcatc
    39001 tcttactgct actaagtgcc tttgagctcc tattttagca gctacaggca ctatatattt
    39061 atccataaat gacatgaatt tttccatttt acatcctcca gtacaacttt attttgaact
    39121 atttaactct acagctctat ctaacacagc ttttccatta catcttccat aatccatcat
    39181 atttataact tctactggaa tattatatgg ttttgccatt tcagttgctt ttcctaatac
    39241 atatctaact tgaggcccta aaagtaagac atcacaattt tctatttcat tttttaagtt
    39301 cacttctgat atagcccata tttttgcttc tataccgttt tcctttgctg catcctccat
    39361 ttttgtaact aataaactag ttgacattcc tgctgaacaa gctaacatta tttttatcat
    39421 tttttcccct ccaaattaga ttcattaaat attttttaag tcataagttt tttaaactat
    39481 acatttaaaa tatgtttttc tattaatttt ttcttaatgt atatatctta tttttaaatg
    39541 tatatacatt taattcttga tattattata taccaaatgt atatacattt caagaaaact
    39601 ttttcatttt atttaaattt tattcgacaa cagcattcaa agttaatgta tatccatagt
    39661 gatatgtgtg tgagtactca aaaatctcac tattttgtaa cttacaaatt tggcttatat
    39721 ataaaagtgg ctctggttct tctaatttta gattatccat acactcttca ttcgatatta
    39781 cagcttgtac ttctctctct gacatggcta tcttgtattc acacacttct tcaacaaaat
    39841 tataaagaga attttcacaa tgagtcttat ttaaatttgg aaataactta actggcatat
    39901 aagtaatcat atatgaaact cttgcatcat tggctaatct tactcttttt atttcccaaa
    39961 cattttcttc taaacttata ttcaacttac ttgataaatt ttcatctgcc tgaactattt
    40021 caagagatac caatatatta gatactttat agcctttact ttccatctct ttagtaaatc
    40081 cacttacaga cgatatatta tcatatatat tatttgcaat aactatacta cctacacctc
    40141 tttgtctagt tatatagcct tctttaacta gattattaag tgcagacctt acagtcattc
    40201 tagttacatt aaattcttca ctcagctgtt tttcagaagg gattaaatcc ccttctttca
    40261 aactgtctga atttattaac tctctaacat gattttctat aactttataa attggctctt
    40321 tcatttaatt cactccttat taaatctttt atataataag ataattttat atacaaacaa
    40381 agaaaatgtc tatacattta agataattta aattttattc agaaatcttg tacaactttc
    40441 cgagttttaa taaagatata aatagactta tcattaatat accttttaat atacttgata
    40501 aggttatact ccaccataca ccatttagac ccaacatttc tggtctagat ataaagtaag
    40561 ccaatggtac tcttgcacca gtcagtatag taattataat agaaggtata taggttctcc
    40621 ctaatccttt gaaagctcct gttgttatta tttctaaaca cataaaaaat tgagaatagc
    40681 ctaatatttt aagataaact gctccttttt ctattgcctc actttcatct ataaaaatag
    40741 aaaatatact ctctcctgca aacactaata ctaaagtagt tattatacca agtacagaag
    40801 atacagttat agctattttg caacctttat ttattctaga atacttcttg gcaccataat
    40861 tttgtccaac aaaactgcct aatgcaactg atagcccttc agccgtcatc caggaaattg
    40921 cttctatttg tgagcctact ttttgtactg caacagccac aggcccccat gatgctacta
    40981 ttactcctaa tagcattgag aatactgtaa acataccact ttgtattgct ataggtaatc
    41041 ccaatttata cagaacttta taatatttag tctctatttc tttaaatagt cttaatttaa
    41101 aatattcttc tttactttta actattatat atataaaaca tgtagtaact actatctgag
    41161 caaatacagt tgcaatagca gctcctgcaa caccaagttt tggagcaaat ccccatccaa
    41221 aaattaatat agggtcaagt ataatattag ttattaatcc tactgtatta atacgaaaag
    41281 gagttttact atttccaaga ccattgaaaa ttgcagtaaa tactggatta ataaaataaa
    41341 ataccatacc tagagctact attatcaaat actgtctaga catggttata acttctaagt
    41401 ctcctaatct aaaaaaacct atcaattgtt tattaagtac tattaaagaa attgtatata
    41461 ttattgctag tataatattt atttctattg ctgacttcat atatgattta gtagtgctta
    41521 tattatttgc accaatactt tgtgctactt ttatctctcc acctactttt gaaatcataa
    41581 taaatgccat tgcaagccat ggaaaaaaac cagctgttcc aactgcagct actgctttac
    41641 ttccggcttt accaacccat atcatatcta tcatgttata ccccatctgt ataaatgaag
    41701 tccccattat tggaagtgat agcttaagta atttttctgt aattcttcct tctgttaaat
    41761 taagtctgtc tttcattttc cacctctttt atattaaatt tatttgctta tatagctaga
    41821 ttaatcattt tatttgattg agttcactat ccttcaattt atattatcat atctcaggat
    41881 tgcatctcaa tcatacttat gtttaagata cagtatttta cctatctatg ataaaattaa
    41941 tacttatgaa tataaatcta gccatttcat caatacatat tctaaccctt aacttaactt
    42001 caattaagta tacatatcgt aaccaattag aatattttat acggtatctt ccattaataa
    42061 acaaccaata tcaatgattt atatataatt ttatttatac ataaaatagt atactataat
    42121 aataaaaaaa ctatatttga atgctttaag catgtttact aattgtaatt tccacacaaa
    42181 tttgataata tatgaatata ttatggtgaa atttatagat atatattaat tatggggaga
    42241 ttgtgggagg ttcttttcac aagacattta agctatgaaa ataaataaaa aattcaatag
    42301 agttgatatg caggtttcaa tacttactgc aattattgta ataatatctt cctcatgtgt
    42361 atattttttt aattatacac taacttataa gagcatgata tactctttaa gtgagaggtc
    42421 aaaacatatc tatgaatatg tagaaagaaa tattgataaa aataccttta ctgaaattaa
    42481 ttctaaagaa gaccaatcaa aaaagtctta taaaagttct aaaatccttt tagaatctgt
    42541 aaaaaatact actggagtca tgtatctata tactgctaaa aaaacaaaag atggatcact
    42601 agtatatgtt gttgatggtt taaattcatc taggagtgat tttagaaatg ctggtgattt
    42661 gatagaaaaa gagatttggg gagaccttaa caaagcatta gataatcata tattgttacc
    42721 taaaaagatt aaatcaacat cttggggata catatttata tcgtattttc ctattcacaa
    42781 tgatgttggt aaggttgttg gagtaatagg aattgaattt gaagcaaaac atcaatacaa
    42841 tacatttaaa tttataagta tagttacacc aataatagca ttattaactt gtctaatatc
    42901 agcattgatt gcagtgattc tatttaaaag aatatctaat cctacatata ctgattttgc
    42961 taatacagat tatttaacag gcctaaaaaa tagaaatgct tttgaaatag atatgaataa
    43021 cttaaacaat tcaaatctaa agaatctaat atctatcata tcaatagatt tagacggttt
    43081 aaaaaagatt aatgataaat ttggccatca gacaggagac ttatatatta agcaagcaag
    43141 taaaattata caatatgtaa taaataaaaa atatatagtt tatcgtattg gaggtgatga
    43201 ataca
//
LOCUS       DBGJFU010000019        42463 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.019, whole genome shotgun sequence.
ACCESSION   DBGJFU010000019 DBGJFU010000000
VERSION     DBGJFU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.019"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            194..1027
                     /locus_tag="ACOIAS_000987"
     CDS             194..1027
                     /locus_tag="ACOIAS_000987"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2700706.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1096..2052
                     /locus_tag="ACOIAS_000988"
     CDS             1096..2052
                     /locus_tag="ACOIAS_000988"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2700707.1"
                     /translation="MNKKLYIGMVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2104..2586
                     /locus_tag="ACOIAS_000989"
     CDS             2104..2586
                     /locus_tag="ACOIAS_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2700708.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2598..3467
                     /locus_tag="ACOIAS_000990"
     CDS             2598..3467
                     /locus_tag="ACOIAS_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HGZ2700709.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3510..4265
                     /locus_tag="ACOIAS_000991"
     CDS             3510..4265
                     /locus_tag="ACOIAS_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HGZ2700710.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4307..5128
                     /locus_tag="ACOIAS_000992"
     CDS             4307..5128
                     /locus_tag="ACOIAS_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HGZ2700711.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5133..5894
                     /locus_tag="ACOIAS_000993"
     CDS             5133..5894
                     /locus_tag="ACOIAS_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGZ2700712.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLA
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6055..6312
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6425..6664
                     /locus_tag="ACOIAS_000994"
     CDS             6425..6664
                     /locus_tag="ACOIAS_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGZ2700713.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            6917..7963
                     /gene="ilvC"
                     /locus_tag="ACOIAS_000995"
     CDS             6917..7963
                     /gene="ilvC"
                     /locus_tag="ACOIAS_000995"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGZ2700714.1"
                     /translation="MSNKNKYHKNKKKYWEEMKMARMYYEKDVDLEVLKNKKVAVLGY
                     GSQGHAHAQNLRDNGVHVVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPD
                     EKQKKVYEESVKDNLKEGQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVF
                     TKGSGVPALFAVYQDYTKKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVL
                     CGGISELIKLGYKTLVDAGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEY
                     GDYVSGKRVITDAAKQGMQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIA
                     EVGRNLRSMMSFLK"
     gene            8016..9704
                     /gene="ilvB"
                     /locus_tag="ACOIAS_000996"
     CDS             8016..9704
                     /gene="ilvB"
                     /locus_tag="ACOIAS_000996"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HGZ2700715.1"
                     /translation="MSGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10065..11195)
                     /locus_tag="ACOIAS_000997"
     CDS             complement(10065..11195)
                     /locus_tag="ACOIAS_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2700716.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINRKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKIKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(11435..11941)
                     /locus_tag="ACOIAS_000998"
     CDS             complement(11435..11941)
                     /locus_tag="ACOIAS_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGZ2700717.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(12204..13793)
                     /locus_tag="ACOIAS_000999"
     CDS             complement(12204..13793)
                     /locus_tag="ACOIAS_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HGZ2700718.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IVFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            complement(13817..14380)
                     /locus_tag="ACOIAS_001000"
     CDS             complement(13817..14380)
                     /locus_tag="ACOIAS_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700719.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            14533..14754
                     /locus_tag="ACOIAS_001001"
     CDS             14533..14754
                     /locus_tag="ACOIAS_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700720.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            14757..15572
                     /locus_tag="ACOIAS_001002"
     CDS             14757..15572
                     /locus_tag="ACOIAS_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGZ2700721.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSDGMTDVKMTTKWTQKGRLFLYDLLKVNNILPDIEKEYSYQTSMLC"
     gene            15621..15749
                     /locus_tag="ACOIAS_001003"
     CDS             15621..15749
                     /locus_tag="ACOIAS_001003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700722.1"
                     /translation="MSNRKKYTLSITKELYNKCKEKANQRGMSVNDYILSVISKSI"
     gene            complement(15751..15930)
                     /locus_tag="ACOIAS_001004"
     CDS             complement(15751..15930)
                     /locus_tag="ACOIAS_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGZ2700723.1"
                     /translation="MSTSLPKYTLRINRVLLEKIKYIAESEGRSANKEIEQIIKKHIE
                     DYEQRKGEIKINIEE"
     gene            16066..16245
                     /locus_tag="ACOIAS_001005"
     CDS             16066..16245
                     /locus_tag="ACOIAS_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700724.1"
                     /translation="MNSNKKRITVRMPEKLNEEITKKSKYLGLTKNSFILDILWKEFE
                     LLEYRSYKKEVDKHE"
     gene            16238..17029
                     /locus_tag="ACOIAS_001006"
     CDS             16238..17029
                     /locus_tag="ACOIAS_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGZ2700725.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDSRNISNNFNNNKDKFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFASKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            complement(17112..17306)
                     /locus_tag="ACOIAS_001007"
     CDS             complement(17112..17306)
                     /locus_tag="ACOIAS_001007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700726.1"
                     /translation="MKFVYVKIFNNWIDITDIGSINGCNAIEYINENLDKMYSSNYVS
                     INYKDKNYCIHPSCIQIVTK"
     gene            complement(17462..17680)
                     /locus_tag="ACOIAS_001008"
     CDS             complement(17462..17680)
                     /locus_tag="ACOIAS_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700727.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYEAGSREPN
                     LETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            17793..17987
                     /locus_tag="ACOIAS_001009"
     CDS             17793..17987
                     /locus_tag="ACOIAS_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700728.1"
                     /translation="MKINNNLKLQREKIGLTQLEVAQKAKITERSYQYYEAGERLPNI
                     RTALKIAIILNTNCEKLFNE"
     gene            18029..18823
                     /locus_tag="ACOIAS_001010"
     CDS             18029..18823
                     /locus_tag="ACOIAS_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HGZ2700729.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEIRHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSTYLDSKNESRPCYNVTKLGCDFLANKFTGEKGIIFTARYVRRF
                     NEMERVLKEQQPKLPTTYKEALQQLLVEVEEKEQLQLENQEKDKVIQLQQPKVLFADA
                     VSASNNSILVGELAKLIKQNGVDIGQNRLFTWMRENGYLIKRKGEDYNIPTQKSMNLR
                     IMEVKKRVINNPDGSTKVTRTVKITGKGQVYFVNKFKSSKQLSMLS"
     gene            18880..19089
                     /locus_tag="ACOIAS_001011"
     CDS             18880..19089
                     /locus_tag="ACOIAS_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700730.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(19099..19473)
                     /locus_tag="ACOIAS_001012"
     CDS             complement(19099..19473)
                     /locus_tag="ACOIAS_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6173 family protein"
                     /protein_id="HGZ2700731.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            19549..19746
                     /locus_tag="ACOIAS_001013"
     CDS             19549..19746
                     /locus_tag="ACOIAS_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700732.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELNIDVSGLYKLLKNKNSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            19777..20169
                     /locus_tag="ACOIAS_001014"
     CDS             19777..20169
                     /locus_tag="ACOIAS_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700733.1"
                     /translation="MDISESITKQFSDSLKSLIEIEINKQETDRVKSQTVEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTVFMRRLVSTGLIKSLARGDGRKISRYEV
                     DDFIERNQGKNLDELLKVAERGDEIVKP"
     gene            20156..20299
                     /locus_tag="ACOIAS_001015"
     CDS             20156..20299
                     /locus_tag="ACOIAS_001015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700734.1"
                     /translation="MLSLNTNKNNIVTLKKDGKAIADIVFKDIKTGKKISVGVLNKKV
                     LVK"
     gene            20466..20708
                     /locus_tag="ACOIAS_001016"
     CDS             20466..20708
                     /locus_tag="ACOIAS_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700735.1"
                     /translation="MEAARLIAIGQIKQAEKEICKLQGTKNNSSLMWWEAVKFASQNI
                     LEGLEHDIELEASIEFREAMMYQEELEKDRPIDVQI"
     gene            20787..21068
                     /locus_tag="ACOIAS_001017"
     CDS             20787..21068
                     /locus_tag="ACOIAS_001017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700736.1"
                     /translation="MKTKNEIIKGLEDRLFLLRFTTVDEVDWDVKFGQISALEFCIDK
                     HRKGCTLEQFKEHLDEYKLQGNYGDYIDGFVSVLERNIREMEGEIDGSE"
     gene            21055..21693
                     /locus_tag="ACOIAS_001018"
     CDS             21055..21693
                     /locus_tag="ACOIAS_001018"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HGZ2700737.1"
                     /translation="MEVNNIYIKLMDVRIKFNKLDIKKSGQNKFANFKYFELADFLPQ
                     ATELLQEAKLCPIVTFTNDYATLTLINGEKPTEEIIFTSPMRELQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDTFDATSGKEDYKRNDLTNSSIKASENGQIKISQNQIKRL
                     FSIGNTIGKDSDRVKSEVYYKFNKEVKDLSKQEYDQICVGYEKLQREKGIIK"
     gene            21703..22161
                     /locus_tag="ACOIAS_001019"
     CDS             21703..22161
                     /locus_tag="ACOIAS_001019"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700738.1"
                     /translation="MKDRDKATIEKGNILSDGYGLSPQLVARDSWLTTGARALYFYLS
                     SFAGASGTCYPSRDIMTHELGINKDTFSKYLNELKTSGYIKVYKNKTREGRMQNNIYE
                     VVFDRSYIESHISIRCKKENKKNHVRKKQTWNHVRIYRTWFNRTWKSRTL"
     gene            22098..22598
                     /locus_tag="ACOIAS_001020"
     CDS             22098..22598
                     /locus_tag="ACOIAS_001020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGZ2700739.1"
                     /translation="MEPCPNLPDMVQPDMEKSDTISNSIINNSFKSSMYIEQAVDNSL
                     KEFKKLYEENIGVVYPVTAEWLLEVSNEVDIRVFKRAIEICAERMNMNLSYLKGILKK
                     WKDANITTYEQLESYKLQHENKKSKKTNSVVSKNKFANFEQTFTQYSNKELDEIIKKS
                     QKAKFK"
     gene            22618..23037
                     /locus_tag="ACOIAS_001021"
     CDS             22618..23037
                     /locus_tag="ACOIAS_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGZ2700740.1"
                     /translation="MNQVVLVGRLTRDPELKYIPGTGTAVASFTIAVDRNYINKEGKR
                     DTDFIPIEVIGKSAEYCANYITKGKLVALEGNIRVDNYQTQSGEKRIFTKVSTKSVQS
                     LESKNKSSNSYKESVQDGTIGLDPQGFEIIDDDELPF"
     gene            23067..23429
                     /locus_tag="ACOIAS_001022"
     CDS             23067..23429
                     /locus_tag="ACOIAS_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700741.1"
                     /translation="MFKVERYFSGSVVDNLIEDDLTCRNYLALYCCLLGITKNGKKIY
                     PKPEKMLAEFGVKKDRKIKKELPVRIRNVNTGEVKEFESIDGAACFLRLKYQAVYQAI
                     KKKTKTRSGWKVEYIKEE"
     gene            23429..23563
                     /locus_tag="ACOIAS_001023"
     CDS             23429..23563
                     /locus_tag="ACOIAS_001023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700742.1"
                     /translation="MEVSRTEYTIKRAKELYDNGEDIFIAIDKAREKYEEMIKSEYLS
                     "
     gene            23663..24514
                     /locus_tag="ACOIAS_001024"
     CDS             23663..24514
                     /locus_tag="ACOIAS_001024"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGZ2700743.1"
                     /translation="MNVLSLFDGISCGQVAFERARIKVENYFASEIKEIAIKVAMNNY
                     PKTIQLGDVRELNVSKIPKVDILIGGSPCQNLSKGRLIANKIQDGLTGDKSNLFWEYI
                     RILKQLNPEYFLLENVVMPKKDENMISQLLGVEPIKINSNLVSYQNRDRLYWTNILNV
                     QQPKDLKISFQDYKDTSFDYCKEFEIKKTPSREKMWGNGLNGNCKNVTYEDKINCVTL
                     KQDRFSNSGLVEFGSFCRYLTTRELELAQTLPVGYTKGLSIRQAQNVIGDGWTIDVIA
                     HILSNIN"
     gene            24831..25028
                     /locus_tag="ACOIAS_001025"
     CDS             24831..25028
                     /locus_tag="ACOIAS_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700744.1"
                     /translation="MNKFQKAVSQMVKQEEKENLLQGYENCIVGRSISSSIRRYVKGF
                     EKFGYSVHEVYEFINDINKYE"
     gene            25055..25471
                     /locus_tag="ACOIAS_001026"
     CDS             25055..25471
                     /locus_tag="ACOIAS_001026"
                     /inference="COORDINATES: protein motif:HMM:NF018101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HGZ2700745.1"
                     /translation="MSKYNNKKIVIDGIKFDSKDESEYYLYLKEKKENGEIKDFGLQQ
                     KFELQPKFKKDGKSYRAITYTIDFAIYKWNGEVVYIDVKGYSTQQGELRKKLFDYKYQ
                     DKKLIWIAKSKKYGVDGWIEYSELKKKRKENKKKVA"
     gene            25490..25990
                     /locus_tag="ACOIAS_001027"
     CDS             25490..25990
                     /locus_tag="ACOIAS_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700746.1"
                     /translation="MASKVKKEFFMATKKHLENYKQLHINIESLKLQIKNLKEFHLGD
                     FMQGLSYDSIPISKTNSISNQVENELINLEEKIIEKQIELYEMEALKYTIDVSISNLK
                     PIHKQIIRYRYIEGLEWSLIVDKVYLEERQLRERANQAISSISIALFGKKALIEQEPL
                     FKMLDL"
     gene            26411..26617
                     /locus_tag="ACOIAS_001028"
     CDS             26411..26617
                     /locus_tag="ACOIAS_001028"
                     /inference="COORDINATES: protein motif:HMM:NF040423.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700747.1"
                     /translation="MNRFEGIYSFAEATKLWDLKDSTLRKAVATGKLVENIDCKKFGR
                     DWVVTIEAMEREYGKLNNIMKDLD"
     gene            27106..28293
                     /locus_tag="ACOIAS_001029"
     CDS             27106..28293
                     /locus_tag="ACOIAS_001029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700748.1"
                     /translation="MLNEKNLKNIKNILQMVQMKHLKDFENWGINKRKKETAINSLIN
                     IIQNDGEKLDYFKEWISIFTIDGHNNYYVFNYNDSDLELEDLKNIVEDIVSENLLEID
                     TSDLEETKVIYKKNDVINKRLIIRFISPALISCKNENENGNIESGLEKTFYFATLFLD
                     LSLKQIIVSIPHTVGIKSIDNIEAKARDFSEIAKYYVSKLEDLLTTYNIQIEKYNDWI
                     YDATYELAEEGSAHNNPEINKKYEENIDKIDEFAKKIAIASGITDKAIIENFKEGVGI
                     LYENILIDEFGVVEDENQYSTFVQNGDGVNSYCRVGSKTATLKSGKGHAIAKTSRNNE
                     DVKNLGVMKSFNGTLSRFLIEVLDKELYLIRTDTSKFIEERVIYDVIRKVGEYKYTFR
                     KSE"
     gene            28238..28624
                     /locus_tag="ACOIAS_001030"
     CDS             28238..28624
                     /locus_tag="ACOIAS_001030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700749.1"
                     /translation="MMLYEKLENISILLGNQSKAKSIIEELEKLMKSDIITPRLLARK
                     TKVTLLEATRVLEFLVKENELEFFIVVECSNPDSVYENEIGHYKYFTSIKEFNAFSKG
                     QECPICGCGYKYNFEDTKIGFRRLEK"
     gene            28629..29399
                     /locus_tag="ACOIAS_001031"
     CDS             28629..29399
                     /locus_tag="ACOIAS_001031"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGZ2700750.1"
                     /translation="MSLDTLKEYLSICDDNLIWEPFYDDSMLLDEFSNYIKEIELSKE
                     WNKSKNHEKGKILEQLVRFIMSRFTILSDISVNKSTTDNELDVFVKFNDNIPIPFLCN
                     IKSKIVCECKNWKSRKVDVGMVSKLAEICNKNNAGLGIFISLNGLTGKGWQYAEGKRR
                     KLYLSDKMPIISFTVDEITKLKYKRYNLLTMIKCKMQALIDEMEFAGGELRLLESELG
                     FIDCLNENIESLKMLGLITEVEYNNIKVRINHRYDIVN"
     gene            29457..30140
                     /gene="terS"
                     /locus_tag="ACOIAS_001032"
     CDS             29457..30140
                     /gene="terS"
                     /locus_tag="ACOIAS_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HGZ2700751.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWANE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKDKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKYESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
     gene            30130..31371
                     /locus_tag="ACOIAS_001033"
     CDS             30130..31371
                     /locus_tag="ACOIAS_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HGZ2700752.1"
                     /translation="MIVRIDFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDYILKL
                     GDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIKSKVT
                     GNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGILTNP
                     NLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQMRKEQ
                     DPEGYKVYGLGEWGETGGAILKNYVIHEFPTESEYFDNMRLSQDFGFNHANVVLRIGF
                     KDGELYICNEIYAHEMDTSEIIKIANSIGLEKTLFMYCDSAEPDRIKMWKSAGYKAKG
                     VKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEFMDDA
                     MAALRYSIDNKLKNNGISFLK"
     gene            31377..32816
                     /locus_tag="ACOIAS_001034"
     CDS             31377..32816
                     /locus_tag="ACOIAS_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGZ2700753.1"
                     /translation="MLNIYISETDLIKVQLKKESTINLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDITYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            32806..34299
                     /locus_tag="ACOIAS_001035"
     CDS             32806..34299
                     /locus_tag="ACOIAS_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HGZ2700754.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWAIDGLNFSERIWGKYRPTLVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLEVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKNNSKQTGAKYTKGDIEWNIRREEEAELYYDNIRNRKDDISKISKNTNWSE
                     KSIGQIKNHIFYNTHIMRDGTRRMLDSDYSMSVAWQRLINGTYEDIDILLLKHEYLES
                     IFEKKYNISNLEAHRMTEKKHDWYKELIKQKGEFEEDDCLNELIRKE"
     gene            34265..34507
                     /locus_tag="ACOIAS_001036"
     CDS             34265..34507
                     /locus_tag="ACOIAS_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700755.1"
                     /translation="MIVLMNLLEKNNEYVIYSYGYEENKLDGRIKIYLDDFYNYEIIK
                     ESKDEHISKSATLKAISKLIKAAKNNDLKKEMSYQC"
     gene            34578..34772
                     /locus_tag="ACOIAS_001037"
     CDS             34578..34772
                     /locus_tag="ACOIAS_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700756.1"
                     /translation="MYILTQVIAVVCVVQIFINCIANVNVGILCNKLKEKNEANIDKV
                     SDEILKRVGEELNESLDKSL"
     gene            34817..35380
                     /locus_tag="ACOIAS_001038"
     CDS             34817..35380
                     /locus_tag="ACOIAS_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HGZ2700757.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            35392..36282
                     /locus_tag="ACOIAS_001039"
     CDS             35392..36282
                     /locus_tag="ACOIAS_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HGZ2700758.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKFFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            36301..36534
                     /locus_tag="ACOIAS_001040"
     CDS             36301..36534
                     /locus_tag="ACOIAS_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700759.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            36544..36945
                     /locus_tag="ACOIAS_001041"
     CDS             36544..36945
                     /locus_tag="ACOIAS_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700760.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            36939..37286
                     /locus_tag="ACOIAS_001042"
     CDS             36939..37286
                     /locus_tag="ACOIAS_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700761.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            37286..37576
                     /locus_tag="ACOIAS_001043"
     CDS             37286..37576
                     /locus_tag="ACOIAS_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HGZ2700762.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            37686..38492
                     /locus_tag="ACOIAS_001044"
     CDS             37686..38492
                     /locus_tag="ACOIAS_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700763.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNNLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            38841..39278
                     /locus_tag="ACOIAS_001045"
     CDS             38841..39278
                     /locus_tag="ACOIAS_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HGZ2700764.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEIDEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKNNDINKMDTVELFGGLKRGDNFE"
     gene            39262..39447
                     /locus_tag="ACOIAS_001046"
     CDS             39262..39447
                     /locus_tag="ACOIAS_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700765.1"
                     /translation="MIILSKTLSKGTDYKFTKEQIVNSKKYINRKDLLNAILKENELY
                     SFSEVEEIINSFMKGVS"
     gene            39449..40759
                     /locus_tag="ACOIAS_001047"
     CDS             39449..40759
                     /locus_tag="ACOIAS_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HGZ2700766.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDIGLVESSLIYWAAGVIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVN"
     gene            40776..41246
                     /locus_tag="ACOIAS_001048"
     CDS             40776..41246
                     /locus_tag="ACOIAS_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGZ2700767.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            41318..41758
                     /locus_tag="ACOIAS_001049"
     CDS             41318..41758
                     /locus_tag="ACOIAS_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGZ2700768.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            42099..42260
                     /locus_tag="ACOIAS_001050"
     CDS             42099..42260
                     /locus_tag="ACOIAS_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700769.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
BASE COUNT        17510 a         4436 c         7512 g        13005 t
ORIGIN      
        1 atactcaatt ttcaacattc tatacaatta tacagaaatt atacagcata ataatcaccg
       61 aaataacaaa ttttaaaaat taagcaaaac tttttgtcga agttcaacgt ctatatatat
      121 gtagtgtaaa tttttgggat aaaatactac aaaaatataa atgtaaaaat atagtaatat
      181 tggaggtcga acgatgagaa ataaaaagaa aaaaataaat agaaagaagt tgtatttgct
      241 tttaagtgct attgtagtgt gtctggcttt tatagtagtt ggtgttcgcg tcagctcttt
      301 aaatatgaag gaaaatgaaa ttagtagaaa tgcaaaactg tcaagttcta ctattacaga
      361 tatagtatct aatacatcaa tagaagttag taaagggcca agtaaaagtt ctggaaaaat
      421 agcatacata actatagatg atggtccatc taaatttact gacgagatga taaaaacttt
      481 aaataaatac aatgttaaag caactttttt tatgatagat gggaatatga aggaatatcc
      541 acagcaagtt aaaaatataa ttaaaaatgg gaacacagca ggttttcata gtgtatcaca
      601 tgatattcac aagctttatg ttactagtac atcagcaaaa gaggaatttg atacaaatga
      661 tcaaactttt tataagataa ctggtaaaca ttcaaaagta ataagaatac cttatggaag
      721 caaaccatat acaccacaag cctcttatca ggctttagtt gatgctggat ataaaatttg
      781 ggactgggac ttagatacag aagactggag atctaattct tctcaaattg tacaaaatgt
      841 aaaaaatcat attaagaata gaaaaggtga agataaagac caacttgtag ttttaatgca
      901 tgagaaaaaa cagagtgcag aagctttaga ttcagtttta aaattcttat cagatgaagg
      961 ttatgaattt gcgccagtag atcaaaacca aataccaaaa aattattggt tacgtaattt
     1021 agagtagtta aaatataaga atattgattg atatttttag aaattgagaa taaaaatgag
     1081 tgaggtgtag gtatattgaa taaaaaactt tatataggaa tggtcggaat attgtctttg
     1141 atgatggttg gttgtaataa aagtttagca aaagtaaatg atgtggaaat cacaaaagaa
     1201 cagtacaaaa aaacaaaagc agttttatct gctactaata attatataaa tgggcagtct
     1261 ttagatgaat tagaaaaaac tttggataaa aaaggtagaa ataaattaga aaatgttata
     1321 atatcgttta tggtggataa tgaactttta tatcaagagg ctaaggataa aggactaact
     1381 ccaagtaaaa gtgaagtaga ctcaaaatat caagaactag aagataagat gaatttgaat
     1441 acaagctata aggaaaaaat ggacaaagca ggtgttgata aagattattt aaaacaagaa
     1501 atatctagag atttagcaat agataaaaat aaaaaggcct ttgaagatag aataaacata
     1561 agtgataatg atatggaagc ctactataca agtcataaaa aagattttaa tgtagaagaa
     1621 gtaagtgctt ctcagatact tatatcgact ttagataaaa ataaaaaaga agtaagcaaa
     1681 gataaaaaag aagctttgaa gaagaaggca gataatatat tgactaaaat caaaaatgga
     1741 gaaagttttg aaagtttggc taaaaaatat tcggatgata aagcaactgg aaaaaatgga
     1801 ggacagctag gatattttac aaaggatgat aaaaatgctg agtttacaaa ggaagtgttc
     1861 aaattaaaga aaaatgaagt ttcaaatgtg tttgaaacta gttatggata tcatatagtc
     1921 aaagttactg ataaaagaga aagacaaaaa agttttaatg agtgtcagag tttaataaga
     1981 gaatctattt taaatgaaaa gtatattgag catataaaaa aactgaatga agatgctaaa
     2041 atagacagat agaatttcta acagctcaat cctagcctgt aatcggaggg agattttaca
     2101 ggaatgttta agagtttaaa gagaacgaaa gttgagaaat acattatcga taataaagac
     2161 tctttttata gaatagcata tagttatacg aagaatgaag aagatgcctt agatgtggtt
     2221 caagaagcca tgtataaggc actatactca gtggaaaata ttaaagaagt aaattatata
     2281 aagacatggt tttataaaat tttagttaga acgagtatag attttattag aaaaaaccgt
     2341 aaatataata atatgacaga tattgacctt atagatgaga ctggagagta tgataaatat
     2401 actgatttgg atttaagaag agctttggaa gaattaccta ttgaatacaa atctattata
     2461 atattaagat tttttgaaga tttaaagatt gaagaagtag caataatatt ggatgaaaat
     2521 gtgaatactg ttaaaactag gttatatact gctttaaaaa aactaaaatt aaaaattgaa
     2581 gagtagggat aaaaagaatg acaagcaaag aaaggttaaa taagttaaaa gatgagtatc
     2641 ataaaattcc aatacccaag aaattagata ctataataaa tcatgaaaaa atagaaaaca
     2701 tatatcgaga gaaaaataaa aaagtaaata gattaagatt taaagttgca atagcctttg
     2761 catgtatatt tacagtatta gtaaatataa gtcctgtatt tgcagacaat ttttctaaga
     2821 tacctgtaat tggagcaata gtagaagtaa taactataaa aaattatagt ttaaaaagtg
     2881 aaaattatga agctgagatt gatattccta aaatacgagg tttaaaagat aaaaacttag
     2941 aacaaagatt aaacagtagt tttatggaag atggaaaaag gctttatcat caattccaag
     3001 agagaatgga gaagatacaa tcatcaaaga ataaaggata taaatcttta agtttgagtt
     3061 atagtgtaaa aaataattct aaaaaatttc tatcaataga gatgacaaaa aatgagatag
     3121 aggcttcatc atatgtgagt aaggtacatt atactattga taaaaaaaga caaatagttt
     3181 tgactttgcc aatgcttttt aaagatgata agtatataaa agttataagt gataatataa
     3241 aagaacaaat gagagaacaa atgaaaaagg atagtacaaa atcctatttt atagatcaaa
     3301 aaaaagactt gcctgttgaa gattttaaaa caattaataa gtatcaggat ttttatttta
     3361 ataaaaatga agaccttgta atttgttttg atgaatatga agtagcacct ggttatatgg
     3421 gcgcagttga gtttgtaata ccatataaag taataaaaga tttgtaaatt gaatagtagt
     3481 ttatatataa atcttttaag gaggacaata tgactacata taaaaaactt aaaaaatcta
     3541 tatttaaaat tgctatattt tcaatatgct tattagtttc attagtagat acaaacccta
     3601 tatttgcagg tagttccttt aatgaaactt caaatagtga tacgataaag gtgttaaatg
     3661 ctaagagtat caactttaaa caagacaatt atgaagtgaa tgtgagtatt cctaaaataa
     3721 aaggtttaaa agataaaact ttagagaata aattaaacaa tgagtttttg caagatggga
     3781 aaaaaattta cagtgaattt caaaataaaa tgaaaaagga aaataataag ggtcatagat
     3841 acctaaatgt aatccataat gtcaaaaata attctaagga ctttttatca atagaaatga
     3901 caaaagaaga gatagaagct tcctcatcta taactaagaa gcattacact gttgataaaa
     3961 atagaggaat actattaaat ttaccaatat tttttaaaga tagtagatat attagagcta
     4021 taagtgataa tataaaagag cagatgagaa atcaaattaa gaaagatgat acaaaatctt
     4081 attttataga ccaagataaa gactcgccag tgaaagattt tgaatctata aataagtatc
     4141 aagattttta ttttaataaa aatggaaatc ttgtgattag ttttgatgaa tatgaagtag
     4201 ctcctggtta tatgggagca gtagaatttg tgataccaaa taaagtaata aaaagactaa
     4261 gatagatata tagaagattt ttagatagac tggtaaggag aattatatga atatatatga
     4321 acaagcaata agattaatag aaaataatga ggatttcgca tttgctacaa taacgtctca
     4381 ttcaggttca acaccacgtg aaactggagc aatgatgatt gtgaaaaatg attctactat
     4441 atttggctca gtaggaggag gtagtgtaga agcagaatgt ataaaacatg caattaatgt
     4501 tataaaaaac agagaatcaa tgttgtataa atttactcta aacaaatctg atgtggcaaa
     4561 actaggaatg atatgtggag gtactggaga aatacaaata gattttatag atagtaaatt
     4621 aaaaagtaat atagaaaaat ttaataagag actaaaggaa aatacaagta aagcatatat
     4681 atttggtgca ggtcatattt caagagatgt tgctgtgata ctatctcttt tagaatttag
     4741 aacagtagta atagatgata gagaagaatt tgctaatcat gaaaggtttc cagaatcaga
     4801 agtagtagtt ttagattctt ttgaaaacat accagatttt cccactgacg aaaatagtta
     4861 tataattatt ttaacaagag gtcatctata tgattcaagt gctttagaat gggctcttaa
     4921 aagagaggct ggttatatag gtatgattgg aagtagaaca aaaattggat tgacttatga
     4981 aaaactcatg aaaaaaggtt ttaagaagga agaattatca aaagtacatg caccaatagg
     5041 cataaaattg aatgctcaaa caccagctga aatagctgta tgtatagctg ctgaacttat
     5101 aaattgtaga gctaataagg aaaaataaac taatgaacac agggagtaaa gatattgata
     5161 ctttaaaggt taattttaga gggattgaaa taaaagcagt tttagtatat aaaaatagaa
     5221 aaaatatttc tataaaaata gatccatttg gcaaaataac tgtaatgagt cctcctaaaa
     5281 tatccaaaaa aataataaaa gatataatta ttgaaaaagg agactggata cttaaaaagt
     5341 cagaaaaatg taaaggaaga gaggaagtat ataaacaaag aatgtttata actggcgaaa
     5401 aatttcttta tttaggtgaa gagtaccatc tagttataaa agaactgttg agagattctg
     5461 tcaaaaaaag taactgtaga ataactatta acgaatatca aatagttgtt cagacaaatg
     5521 atacaagtac agattttatt aaaaaatctt taaaatcttg gtataagatg gaaagtgaaa
     5581 gaatagtatt agagagaata gattttctta aattaaattg tgaaataatg aagcaattag
     5641 ttccagcgag tgttaaggta aaagaacaaa ataagagatg gggaagttgt acagctcaaa
     5701 aaaatattta tataaactca aaaatatcta tggcaagatt agatgtaatt gattatgtaa
     5761 taatacatga gtttagccat ttagcacata tgaatcactc taaaaaattc tatgatttag
     5821 taaaatccat tatgcccgat tataaagata aagaaaattg gctaaaaata aatggatata
     5881 aaataataat ctgaaaacga ttattccaaa atataggcgt aaagtataaa agaagtatta
     5941 ttgtgaaaaa aataaataat ctgaaaattt taaaaaaagt cattgacatt gtaaatgata
     6001 gagaatataa tctatattaa caacaaaaca gaattgaaat ataaaattaa aaaattaaaa
     6061 gcaatgacag agacaacaat actaatagtg ttatccacag agaaccaatg ttgctgagaa
     6121 ttggtgttaa cagtagtata atgatcactc tggagctttt gtactgaata tttttagtaa
     6181 gtacaaacgt atgtcaacgt tacatgacta aggttaaggt aattaattac ccgacctgaa
     6241 aaaagagggg tagatgtatt ttttatatct acaaataagg tggtaccgcg gaatataact
     6301 ttcgtcctta taagtaatat ttatattgct tataaagatg aaagtttttt tattttaagt
     6361 gatataaata aatttaatcc atataaaatt aataaaaaaa taataaaaat aaggagggat
     6421 ttttatggaa aatactgtat atgctaaatt aaaccaagga atagattctt ttttaagagt
     6481 ggctatgact ctaagaagaa gagaggttga tatacaatca ataagtatga ctgtagataa
     6541 tatgaataat tcaggtataa aattaatagt aaatgaagaa aagacatcct tagattctgt
     6601 cttaaaccat atgaaaaaac tacatgatat aagagagata actgttgaaa gaatgagaca
     6661 ttaattaaat atattttata aaactaaaga taaatatgct tattttaaaa taaaaaacta
     6721 aagataaata ttaaataaga aaaatcttag attaaaatat aagtgtcatt gaatttatta
     6781 gtttatcaca gttaataaat ttattagttt gttgtagcta ataaaaactg aaaattctaa
     6841 aattataatt gtaagattat ataaatttag aatttaaatc tgttgaataa tattaaaaca
     6901 atgataatga gtatgaattt ctaataaaaa taaatatcat aaaaataaga aaaagtattg
     6961 ggaggaaatg aaaatggcaa gaatgtatta tgaaaaagat gtggatttag aggttttaaa
     7021 aaataaaaag gttgcagttt taggttatgg aagtcaagga catgcacatg cacaaaatct
     7081 tagagataac ggtgtacatg tagtgatagg tctttatgac ggaagtaaat cagcacaaaa
     7141 agcaaaagaa gatggatttg aagttaagag tgtagcagaa gctacaaaag aaagtgattt
     7201 gacaatgatg ttaatgcctg atgaaaagca aaagaaagtt tatgaagaga gtgtaaagga
     7261 taacttaaaa gaaggtcaga cattagcttt tgctcatgga ttcaatatac attataatca
     7321 agtacaacca ccagaatttg ttgatgttgt aatggtagct cctaaaggac ctggtcactt
     7381 agttagaaat gtatttacaa agggaagtgg agttcctgca ctatttgcag tatatcaaga
     7441 ctacactaaa aaagctacag agacagtttt agcatatgca aaagggatag gagcaacaag
     7501 agcaggtgtt ttagaaacta cttttaaaga agaaacagaa acagatttat ttggagaaca
     7561 atcagtactt tgtggtggaa taagtgaact tataaaattg ggatataaaa cgcttgtaga
     7621 tgcaggttat caaaaagaag tagcatattt tgaatgttta catgaaatga aactaatagt
     7681 cgaccttata tatgaaggtg gatttgaaag aatgagatat agcataagtg acacagcaga
     7741 atatggagat tatgtatctg gtaaaagggt aataactgat gctgcaaaac aaggtatgca
     7801 aaatgtttta gaggatatac aaaatggaaa atttgcaaaa gcgtggatta aagaaaatga
     7861 agaaggacgt gaaaacttcc taaaaactag agaagaagaa tataatacag aaattgccga
     7921 agttggtaga aatctaagaa gtatgatgtc attcttaaaa taaatatata ttaaatggat
     7981 tagtttgagg gaaagagtgg tgtatagtag tgcgtatgag tggagcaaag gtaattttag
     8041 aatgtttaaa gaaagaggga attgacacaa tatttggata tcctggtgga gccgtgatac
     8101 ctctttatga tgctctatac gattattcag atgattttaa acatataagg acatcccatg
     8161 aacaaggttt agttcatgct gcagatgggt acgcaagaag tactaataca gtaggagttt
     8221 gttttacaac atctggtcca ggagcaacaa atgccataac tggaatagca acagctttta
     8281 tggattcttc acctatggtt gtaatttcag ggcaggtgcc aactagttta ttaggaaaag
     8341 attcttttca agaaattgac ataactggag caacattatc tatgacaaaa cataattatt
     8401 tagttagaaa cactaaagaa ttagttccta caataaaaga agcttttaga gttgcaaatt
     8461 caggtagaaa aggacctgta cttgtagatg ttccaaaaga tttattttta gcagaaatgg
     8521 attttagtgg tgaggactat gatttatgtc aaatagatga ttatatggat tataagagtg
     8581 attttgatct agatgatgaa actaatatta agcttttaaa tgaagcaata gatatcataa
     8641 aagaatctaa aaagcctata atatatgcag gtggaggtgt taaatcttca gacagtgagg
     8701 aaattcttga aaaatttgca actaagatag atacacccgt actaaataca cttatggggc
     8761 ttggaaatat agatagaaag aatgaattat ctcttggaat ggttggaatg catggaagta
     8821 gagaaagtaa tttagcactt tctaattcag atttagtaat agcgataggt gcgagattta
     8881 gtgatagagt gataagtaaa agttctgaat ttgctaaaaa tgcaaaaata atacacatag
     8941 acatagaccc atctgaaata agtaaaaata tagaatctaa tgtatctttg gttggagatg
     9001 taaagctagt ccttagttta ttaattgaaa gggttgaaag taagaataat agcaattgga
     9061 aagaagagat aaagagattt agaaagagtg agggtgtaca aacatatgaa tttcatccac
     9121 aaaatatact aaaaaaaata aatgaaaaat atgaaacatt aaagaaaccc actgtggtag
     9181 taactgatgt cggtcaacat caaatgtggg ctgctaagta ctggaatttt aaaggtaata
     9241 aaagttttat aacatcagca gggttaggaa ctatgggatt tggtttaggt gcagctattg
     9301 gaactaaagt tggtaatgta gataaaaatg tagttttagt cactggtgat ggaagtttta
     9361 gaatgaactg taatgaacta gctacagttg caaattataa tgtacctatg cttattttat
     9421 tactcaataa tagaacatta ggaatggtaa ggcaatggca aaagttattt tccaatcaaa
     9481 gatactctca aactgacatt aatgaaaatg tggattatgt taaactcgta aatgcatata
     9541 atattgatgg atataaggta tccagtatgg aagaattagg aaaggcgcta gatatgatag
     9601 attttaataa acctgtattt ttgcaatgtg atatagataa ggattatgat gtttatccta
     9661 ttgtagcacc taatgatgca ttggaaaatt tgatatgtaa ctaaaactta tactgaatat
     9721 tgataaaatt ttttattaaa tttttattgg tattcagttt ttgttgttta gagatagatt
     9781 ggcatttttt atttttgcaa ataaatcaat gactatataa ttaagaactt attaaatttt
     9841 aattatacaa tttagaatag tattacaact taagtaaata ttaagtttta tttgttagat
     9901 ttattattaa taccttatat tagttatggt tgttatatac tctaaagcct tgaaattaca
     9961 agtatataga tgattatata aatttgataa ttttagcttt taaaatagat aaattagatt
    10021 ttgccaccca tttgccaccg tattatattt ttgggtggca aactctaatt tgtaattttt
    10081 tcaaatatat ctacagtttc atttttcatt ttatcagtta catgtgaata ggtatccatt
    10141 gtagttgata gttggctatg gcccaaacgg ttttgtatgt ctttaatgtt agcaccattt
    10201 tctaataata gagtagcatg tgcatgtctt aaagaatgaa aatggaagtc attgtttaaa
    10261 gctactcgaa tttgtcttac tatagtgtct aaagtgtgag tattcacttg ttgaccattt
    10321 tctttggtac atacccagtc actatcaaag taaaattctc catattttaa tttcattttc
    10381 ttttgatata atttatgttc ttttaatgcc tttattaaag tgtcacctgt aaatatagtt
    10441 ctgcaagagc tttctgtttt tggttgccct aattcaaaca ttccatttgg ttttttaatc
    10501 aaagtatgtc ttactgtgat agttttatta tcaaggtcta tattatccca ttttagtgca
    10561 ataatttcac ctcttcgcat accagtatga aatccaatta gtaaaactat acgttgaaat
    10621 gaatcttgag gaaatatatt tagtatttga ttaaattctt ctaatgtaat agttttaatt
    10681 ttattagttt ctgttttaga tttagttttt ggtatgctca catattgcat agggttttct
    10741 cgtatatgtt tgtaaggatg gactgctgat tttaatgacc tatgtaatat ggcttttaat
    10801 acttgtaatg tattttgaga gtaatcctct ttgtactttc tgtttatgaa gttttgtagt
    10861 attgcaggag ttaaagcttt tactttgtaa gctcctagtt ttggctttat atgtttttct
    10921 atgtttattc ggtagctttc ttgagtgttg tatttacagt taagtaagac atattctttg
    10981 taccaaaagt ctaagtagtc tgataaactg atattgcttt cttcaaatac tatgccagag
    11041 ttttcatatt catttagtgc ttctcttaag gctttttctg cttctttttt agtattgcct
    11101 ccaactcttt ctactttttt tctttttcct tctactatgc ctaggtcaaa gtagtaatac
    11161 catttgttac ttctttttct tactccacct ttcataatag tatccctccc ttttagaatg
    11221 tatgtttgtt tggtgtttat ataaaagagc agattaactg ctctttatat acttttgtta
    11281 tgtacaatat tatcttaatt ttgtttattt ggtttttact taattatttt cttaatattc
    11341 ttacaatttt ttagctttta tgtgtagttt attataataa tattgaaata tatatttatt
    11401 aagtaaaaaa tctataaaag tagaataaat gagtttaaaa aatatcctca tatatgtaga
    11461 aatttggcaa attagttaat actaaaaatc tattatttcc taagtccaac atgcttttct
    11521 tttttgatag aaattctaat ctttttagta agaagttgat gctaacttgt aattcttcag
    11581 ctatttcgta tacacttgta gcgtgtgaat taataacatg tattatttct tcttctgtta
    11641 taagaaattc acatgcccat tttagtgctt tgttttcagt tttgtctatc aagattttat
    11701 ttttgtaact gttttttgaa gatacatagt ttccaacact ggtaaaatga tgtcctaatt
    11761 cttctgctaa gatttctatt agtttagcat tgttttgttt taatgaatta agtaatgata
    11821 taatttttag tccttgtctg tttatataca atccttttat gtcatctgct attttgtcag
    11881 tgtagtaaat ttctatctct tcattatttg ctaagtctaa aagtgcgtct agtttgttca
    11941 ttgaaatccc cctataaaaa gaatgtattt ttgatttttg ttatatataa agagcaggtg
    12001 tgaactgctc taaatattca ttttttatcc aaaaaagatt ggataatttt agctgtagac
    12061 aaaatatgga ttatagtata tgattataat ataattacat ataatatatg taagcactta
    12121 caaatgtgtg ttagtatagt tagcactaaa tttgtatttg tgcttaaagc gattaaagga
    12181 gttttggagc ggtgggtaga gttttactca ctataaccaa gactcttttg catattagag
    12241 tatttcatgt agtcatatat tctaataagc cttcttaaat ttgttttttc gcttgttttt
    12301 gcagactcta aaatattagg attttcctca aatattttct taagtagatt ttctaactgt
    12361 tcagtgtcaa tttctttttc ttctaaaaaa gcaataagtc ttgtagcttt ttcaaatgat
    12421 tgatgttcat cattccaaat accataaaca cttctatttt ttatgtaagt attatttcta
    12481 ttttttttaa tactattgct aataatattt tcaaagtcac tttctagtgc aattgtccta
    12541 taatctggaa attcaagtgt gctattaaaa agatgtttat ttaatggtaa tttattatat
    12601 tgcttaatta aagatggata agcatattca agtaattcat ctgatgaaaa ttctaagtca
    12661 ttcatgacta tatcacgata ccattcatca aaacttaatc cttttaatcc tttaagacca
    12721 aaatcacttg ccttgccaac tgctaaaact aaatcttcat cttttattct tgtatcatca
    12781 acactaccaa cccgaatttt agcagtaggt ttgttagtaa ttgtgaaatc gtaaaattct
    12841 tgtttgaaca ttcttaatat tctaactggt aattttgatt ttttttcaga taatgcctca
    12901 tagagtaaat taaaatcctc cagtttaatt ttagtcatag taatcatttt attatcaaaa
    12961 acaattgtat gacttgatat ttctatatca ttaaagtttt tttcatactc aatgaatatg
    13021 aaacgttcct cgagtttttt agcattttca ttagatagac aattaactat agcattaagt
    13081 atttttttta tatttacatc tgtaacagag taaccaataa atatgatagg aaactcaaca
    13141 aatatagtca ttagcttagc tgccaagtag gcacttttgc tgtcaaagtc tatataatca
    13201 gcttcattta ttacaatact atttggagaa gaaacacatc catgaatttt ataaatttct
    13261 gcaatacctt gtatagatga aaaaactaaa ttttcttgac ctatatatgt tgtataatta
    13321 tcaacgatag tttccaaaaa acaatcataa tttgttgtaa taaatcctga taaacttttt
    13381 ttagagatat tactaagttt ttcaacttca tcttgatatt tatctactat tttagaatta
    13441 tttttaatat acatagcaat ttctgcctta aatggagaaa ctcctgattt aacaatatct
    13501 aaatacttag agtctaagct tcgtatagaa gaatctgaaa accatttttt attaaaatca
    13561 ttttcaataa gttctgcaat tctaggattt atacccattt ttgggttctc tgattttgct
    13621 ttatttttat aacttgtata tataaagtca tcattactaa ttttttctgc aaagactttt
    13681 agtaaatcct cccaatttgg aagattatag tatcttctag ttaaaccaga accaacaaat
    13741 aaaaatggtg tagcattaaa tcggtttatg acatctgata ttatagattt catataattt
    13801 atcacctact tttaatttat tttttatact tatttattaa aaattcaata taatcattga
    13861 gttgttcttg agcttcctct ggtaaatctt tgtgaggatt aactctatgt gcagctacag
    13921 tgtctatatc attttttaca atagacctac ctaataaata atcagtagtc acattaaata
    13981 tttcagcaag gctagatagc atataagcat caggaactgt ctttccttgt tcataataac
    14041 cataagcgct tttggttaag tttaatttat ttgcaacatc ttcttgagtc atattttttt
    14101 cagctctaag ttcttttagt cttttttgaa ataatgtatt attacaagaa gttttgctag
    14161 aaatattgct tgctttatta tcatacctac ctattaaata atcaacagta gtgccaaaat
    14221 attcagctaa actagataac atttctgggg taggtgttct tttaccttgt tcataaaatc
    14281 catatgcact agtagttatg ttaagatagt tggcaatttc tttttgcatt acaccttttt
    14341 ctttcctaag aaatttcaat ctatctgata aaatgctcat ataatcactc ctcttaataa
    14401 agttgccttt attaaataat acaacaaata gttgtaaaat gaaataaaaa aataaaaaaa
    14461 ctatttacaa acaacatata gttgtgtatt atataagtat aaacaacaaa atgttggtaa
    14521 ggaggatata gcttgaacaa tctaaaggaa cttaggacag aaaggaattt aaagcaaaaa
    14581 gatgttgcat taaaagtagg tataactact agttattatg gaatgattga aagtggaaca
    14641 agagtaccaa gtttagcaat agcaataaaa ttatctaaat gttttggagt atcaatagaa
    14701 aaaatttttt taaatacaaa tacaacaaga tgttgtttaa aagagaggag ttaaatatga
    14761 aagatttaaa aatagtaaaa gttaataata aactaacaac tgacagtaga gatatagctc
    14821 taatggttga aaaagaacac aagattttac taagggatat aagaaattat ataaaccaaa
    14881 tggaagaagc caataaaaac atgagtacag atttgtaccc atctgattat tttattgaaa
    14941 atacttattt agatgattat aaaagagaga agccatgcta tgcaataaca aagataggtt
    15001 gtgacttcat agcaaataaa atgacaggca taaaaggtac ggcatttaca ggaatatata
    15061 caaaaaaatt tgaagaaatg gagcaagtat taaaaaatga acaacctaaa ctaccaacta
    15121 catataaaga agcattacaa catcttatag agcaagtaga agtaaatgag aaattacaac
    15181 tagaaggaaa aatgaaagac caagtaataa aagaactaaa accaaaggca gattatacag
    15241 atatgatact taaaaacaaa ggacttgtca caataactca aatagcaaaa gactatggaa
    15301 tgagtggaaa agaaatgaat aaaatacttc atgaaagagg gattcaatat aaacaaagtg
    15361 gacaatggct tttatataaa caacaccaag ggaagggata cactcattca gaaacaatag
    15421 acataactag aagtgatgga atgactgatg taaaaatgac aactaagtgg actcaaaagg
    15481 gaagattgtt tttatatgac ttattaaaag taaataacat attaccagat atagaaaaag
    15541 agtatagtta tcaaacttca atgttatgtt agtactttga aaaataaata cagaatattc
    15601 aaaaagaggt gatttaaagg ttgagtaata gaaagaagta taccttgagt attacgaaag
    15661 aattatacaa taaatgtaaa gaaaaagcta atcaaagagg tatgtcagtg aatgattaca
    15721 tactatcagt gattagcaag agtatataaa ttattcttca atattaattt taatttctcc
    15781 ttttctttgc tcataatctt caatatgttt ttttattatt tgttcaattt ccttattagc
    15841 agaacgacct tcgctttcag ctatatattt aattttttca agcagaactc tgtttattct
    15901 taaagtgtat ttaggtagtg aagttgacat aataatacct cctttttaaa gtctaaatga
    15961 tgtcataatt atatcataaa aataatttat agaaaatcta taaaatatat tgacggcata
    16021 aagacggcgt gatattatta aaacaagaaa ggggttgacg gcaaaatgaa ctcaaataaa
    16081 aagagaataa ctgttaggat gccagaaaaa ttaaatgaag aaataacgaa aaaatcaaaa
    16141 tacttagggt taactaagaa ctcatttata ttagatatac tatggaagga atttgagtta
    16201 ttagaatata gaagttataa aaaggaggta gataaacatg aataacttac aagtaataga
    16261 aagaaataat gagagagttc taactacaca acaattagca gatgtatatg aaactgactc
    16321 aagaaatata agtaacaatt tcaacaataa caaagataag tttattgaag gtaaacatta
    16381 ttttttattg caaggtgatg atttaaagaa ttttaaaggc attcatacag aatatgaaaa
    16441 cctaaaattt gcttcaaaaa tgtatctttg gactgaaaga ggagcaaata gacactgcaa
    16501 aatattagat acagataagg cttgggaaca gtttgataat ttagaggaaa catatttcaa
    16561 agttaagcaa cataagccaa cttgtataga agatgtatta atagaaagtt taaaagaaat
    16621 gaaagattta agacttcaag ttaatcaagc aaatagcatt gctttagaag caaagacaga
    16681 ggttgaaaca ataaaagatg tagtttcatt agactcaaat agttggagaa caaatacaca
    16741 tcaactaatt gcaagaatag caaaaaaaca aggtggtttt gaacatataa atatgcttag
    16801 aacagaaagt tatgaattat taaataagag atttggagtt gacctacata gaagattgat
    16861 caataaaaga agaaaaatgg cagaagaagg tgtatctgaa tctaaaagag agaaagttaa
    16921 caatttagat gtaatacaag atgataagaa actcatagaa gggtatgttg ctattgtaaa
    16981 agatatggct ttaaaatatg gaatatcaag tgatttaagc aaaaattaag ttaaatcaca
    17041 acagcacttt gaaaactaaa tataaaataa aaatatctcc cttaaaagtt ttttaaagag
    17101 agataaacac tttattttgt aactatttga atacatgatg gatgaatgca atagttttta
    17161 tctttgtaat ttatagaaac ataattacta gagtacattt tatcaaggtt ttcattaata
    17221 tattcaattg cattacagcc atttatagag ccaatatcag ttatatcaat ccagttatta
    17281 aatatcttaa cataaacaaa cttcatgata acaccaactt tcattagaat ttaagttaat
    17341 tataacatgg tgtactgaga taatgtgtta agaaatagat acagaatatt caaaagaggt
    17401 gattagatgg aaatagagca aacaacaata cgcctgctaa aaagcagacg aaagttgaat
    17461 attaatttct attttggtta ggcgaaagac ctaacaaaaa atctgtagat acattaagtt
    17521 tgttagaaat taatattaaa gtttctagat ttggttctct tgaaccagct tcataatact
    17581 gatatgcacg ttctgagata ccaaacaaac tagcaaattg cttttgagtc atattcattt
    17641 gctttcttac tagcttaata ttatctttga atttagtcat aactacacgc tccaataaaa
    17701 aaataaataa atttcttgac acgaacaata ttagcgtgta atatataaaa catagcacga
    17761 acaatattgg cgtgttaaat aaaaaggaga gggtgaaaat aaataataat ttaaaactac
    17821 aacgtgaaaa aattggctta acacaattag aggttgctca aaaagccaaa ataacagaga
    17881 gaagctatca atattatgaa gctggcgaac gtctcccaaa cattcgtaca gctttaaaga
    17941 tagctataat tttaaatact aattgtgaaa aactctttaa tgaataatag cataaaagga
    18001 ctcagattac aagaaaggag ctttagatat gaataatttg caacttaaca ataaaaatac
    18061 aataacaaca ttagaaattg ctgatatgtt agaaatcaga cactgggaag tcttaaggaa
    18121 attagaagga acagaaaaaa caaaaggaat tattgatatt cttaacgaca acaattttgt
    18181 tgtggttgat tactttataa aatcaacata tttggattca aaaaatgaaa gtagaccttg
    18241 ctataatgta acaaaattag gttgtgactt tctagcaaat aaatttactg gagaaaaagg
    18301 tattattttt acagctagat atgtaagaag atttaatgaa atggaacgag tattaaaaga
    18361 acaacaacct aaactaccaa ctacatataa agaagcattg caacagttat tagtagaagt
    18421 tgaagaaaaa gaacaattac aattagaaaa tcaagaaaaa gataaggtaa ttcagttaca
    18481 gcaaccaaaa gtactgtttg ctgatgcagt ttcagcttca aacaattcta tattagttgg
    18541 agaacttgca aaacttataa aacaaaatgg agtagatata ggacaaaaca gattatttac
    18601 ctggatgcga gaaaatggat atttaattaa gcgtaaaggt gaagattata atattccaac
    18661 acagaaaagt atgaatctaa gaataatgga agttaaaaaa agagtaatta ataatcctga
    18721 tggaagtact aaagtaacta gaacagtaaa aataactgga aaaggtcaag tttattttgt
    18781 taataagttt aaaagtagta aacaattatc aatgttaagt taaatcataa cagtaccttg
    18841 aaaactaaat acagaatatt ttggaaagga gagattgaat tggaaaataa gaaaatagaa
    18901 gaaatagaga aaagaataac catgcttgaa aatcaattgc aagcacagtt atttagtcag
    18961 ataacagctc aatcaaatag tgattttata gattatatgt accaatgtat agttttgtat
    19021 ttacatgaga tgaataaaag tgaaaaaaga aaaattagtg aaattactaa agaaagaatc
    19081 agaaaataag aatgataatt acaatataaa acctattctt ctagcaggtt ttgaagggtc
    19141 agttttcttt actgaagtta tcataaagtt tatctgtgat gtgtgttgaa gaatttcaga
    19201 ataaactcca tttactatgc cgtagtagtg aattagacaa gggttatgat aacctacttc
    19261 ttcaacaatc attaatacat cttttccaaa gttagtcaat tttagtgcaa tttcatgttc
    19321 atcatcaagt tctttttcaa attcctctat agatttaaga ataattttat attgaaattc
    19381 agcatagtaa aaatactctg tgttagaagt atcagagact gtctttctta tactttcttg
    19441 taattcttta gatatattgt ttgaaaactg cataatacac catccttttg aaaattttgg
    19501 aatttattcc atgtttatat tataccatgt agaactgagg tgaatacaat gttaataggc
    19561 gacaatataa agcaaatact aagaaaaaga gatataaaac cttataaatt agcaaaggaa
    19621 ttaaatatag atgtaagtgg tctatataaa ttgttgaaga ataaaaattc taatccaact
    19681 atagatactc taataaaatt agctgattat ttagatatta cattagacga attagttgga
    19741 agataaatat ttgaaaagag gggagagaat tttaacatgg atatttctga aagtataaca
    19801 aagcaattta gtgatagttt aaaaagttta atagaaatag agataaataa acaagaaaca
    19861 gatagagtta agagtcaaac tgttgaacaa aaggtaaaag tactggagcc taaagatata
    19921 gttgttttaa taaaaagagg ttatccaaat tatttgataa cagtagaaga agcaagggga
    19981 attttaaaat tagatacagt ttttatgcgt aggttagtga gcacaggttt gataaaatca
    20041 ctggctagag gtgatggtag aaaaatttca agatatgaag ttgatgattt tattgaaaga
    20101 aatcaaggta aaaatttgga tgaactttta aaagtagcag agagggggga tgaaattgtt
    20161 aagccttaat actaataaga ataatatagt aactcttaaa aaagatggaa aagctatagc
    20221 agacatagta tttaaagata ttaaaactgg taagaaaata tcggttggag tattaaataa
    20281 aaaagtgctg gtcaaataac caacacacaa aaaaataaaa aataaaatat aacacaaaca
    20341 aattatagca caaaaagaat ggatttagaa taggtaatat aacattctaa aaggtttaat
    20401 gtgtaataat tgtaacaatt tagaatgtag cttatataga tagtaataaa ttgggaggga
    20461 tttaaatgga agcagctaga ctaatagcaa taggtcaaat taaacaagca gaaaaagaaa
    20521 tatgtaaatt acaaggtaca aaaaataata gtagtttaat gtggtgggaa gccgtaaaat
    20581 ttgctagtca aaatatatta gagggtcttg aacatgacat tgagttagaa gcttctattg
    20641 agtttagaga agctatgatg tatcaagaag aacttgaaaa agatagacca atagatgtcc
    20701 agatataaaa aaagagcctt cgcacaggct ctaagtgaaa ataagttaca aaaattatag
    20761 gtatattata acataagggg gaataaatga aaacaaaaaa tgaaataatt aagggtttag
    20821 aagatagatt atttttatta agatttacaa cagtagatga agtagattgg gatgtaaaat
    20881 ttggacaaat atcagcatta gaattttgta tagataaaca tagaaaagga tgcactttgg
    20941 aacaattcaa agaacattta gacgaataca aattacaagg gaactatggt gattatatag
    21001 atggttttgt gtcagtttta gaaagaaata ttagagaaat ggagggagaa attgatggaa
    21061 gtgaataata tttacataaa attgatggat gtaagaatta agtttaacaa attagatata
    21121 aaaaagagtg gtcaaaacaa gtttgctaac tttaaatatt ttgagttagc agacttctta
    21181 cctcaagcaa cagagctgtt acaagaagct aaattatgcc ctatagtgac ctttacaaat
    21241 gattatgcaa ctctaacatt gattaatgga gagaaaccaa cagaagagat aatatttact
    21301 tctccaatga gagaattaca attaaaaggc tctaatgagt tacaagcatt aggaggaatt
    21361 gaaacatatc agacaaggta tttgtacatt cagttattaa acattacaga aaatgatact
    21421 tttgatgcta ctagtggaaa agaagattat aaaagaaatg acttaacaaa ctcctctatt
    21481 aaagcaagtg aaaatggtca aataaaaata agtcaaaatc aaataaaaag attgttttca
    21541 ataggaaata caataggcaa agattcagat agagtaaaaa gtgaagtgta ttataagttt
    21601 aataaagaag ttaaagattt aagtaaacag gaatatgacc agatatgtgt tggatatgag
    21661 aaattacaaa gggaaaaagg aataattaag taggtgaatt tcttgaagga cagagacaaa
    21721 gcaactatag agaaaggaaa tatacttagt gatgggtatg gtctttcacc acaattggta
    21781 gctagagatt catggctaac aacaggagca agagctttat atttctatct atccagtttt
    21841 gcaggagcaa gtgggacatg ttatccatct agggatatta tgactcatga acttggtata
    21901 aataaagata cttttagtaa atacctaaat gaactaaaga cgagtggtta tataaaagta
    21961 tataaaaata aaactaggga aggaaggatg caaaataata tatatgaggt agtatttgat
    22021 agaagctata tagaaagtca tatatccatt agatgtaaga aagaaaataa aaaaaaccat
    22081 gtccgaaaaa agcagacatg gaaccatgtc cgaatttacc ggacatggtt caaccggaca
    22141 tggaaaagtc ggacactata agtaacagta ttataaataa cagttttaaa agtagtatgt
    22201 atatagagca agctgtggat aactctttaa aagaatttaa gaagctatat gaagaaaata
    22261 taggagtagt atatccagtc acagctgaat ggttattaga agtatctaat gaagtagata
    22321 taagagtatt taaaagagct atagagatat gtgctgaaag aatgaatatg aatttatcat
    22381 acttaaaagg aatccttaaa aagtggaagg atgcaaatat aactacatat gaacagttag
    22441 aatcatataa attacaacat gaaaataaaa agtcaaaaaa aaccaatagt gtagtaagca
    22501 aaaataagtt tgctaatttt gaacaaacat ttactcaata cagtaacaaa gaattagatg
    22561 aaattataaa gaaaagccaa aaggctaaat ttaaataata ttgatggagg tattaaaatg
    22621 aatcaagttg tattagttgg aagattaact agagacccag aactcaaata cataccagga
    22681 acaggtacag cagtagcatc atttacaata gctgtagaca gaaattatat aaataaagaa
    22741 ggaaaaaggg atactgattt tataccaata gaagtaatag gtaaatcagc tgaatactgt
    22801 gcaaattaca taacaaaagg gaagctagta gcattagaag ggaatataag agttgacaat
    22861 tatcaaactc aatcaggtga aaaaagaata tttacaaaag tcagtacaaa atcagtacaa
    22921 tcattagaaa gcaagaataa atcgagtaat tcatataaag agagtgtaca agatggaacc
    22981 ataggactag accctcaagg atttgaaatt atagatgatg atgagttacc attttaatct
    23041 gaaaaattaa atatgaggtg aaataaatgt ttaaagtaga aaggtatttt agtggctcag
    23101 tagtggacaa ccttattgaa gatgacctta catgtagaaa ctacttagca ttatattgtt
    23161 gtttgttggg aattacaaaa aatggaaaaa agatatatcc taagccagaa aaaatgttag
    23221 ctgagtttgg agtaaagaag gacagaaaaa taaaaaaaga gttaccagta agaattagaa
    23281 atgttaatac aggggaagta aaagaatttg agtctataga tggtgcagcc tgttttttaa
    23341 gattaaaata tcaagcagtt tatcaagcta ttaaaaagaa aactaaaaca agaagtggct
    23401 ggaaagttga atacattaag gaggaataat ggaagtttca aggacagaat atacaattaa
    23461 aagagcaaaa gagttatatg acaatggaga ggatatattt attgctatag ataaggctag
    23521 agaaaaatat gaggagatga ttaaaagtga atatcttagc tagtgtgata ttagtaatag
    23581 gaagttttat agctggtaga gtttatgagt atagattgaa cctaaaaggg tgtgaaaatt
    23641 gtgataataa gaaggtgcaa atatgaatgt attaagtcta ttcgatggaa ttagttgtgg
    23701 tcaagttgct tttgagagag ctagaataaa agtagaaaat tattttgcaa gtgaaataaa
    23761 agagattgcg attaaagtag caatgaataa ttacccaaaa acaatacaac ttggagatgt
    23821 aagagagcta aatgtaagta aaatccctaa agtagatatt cttattggcg gaagcccatg
    23881 ccaaaattta agtaaaggaa gattaatagc taataaaata caggatggat taactggaga
    23941 taaaagtaat ttgttttggg aatatataag aattctaaaa caattgaatc cagaatattt
    24001 tcttttagaa aacgtagtga tgcccaaaaa agatgaaaac atgataagcc aattgttagg
    24061 agttgaacca atcaagataa atagcaattt agttagttat caaaatagag acagattata
    24121 ttggacaaat attttaaatg ttcaacaacc taaagattta aaaattagtt ttcaagatta
    24181 taaagatact agttttgatt attgcaaaga gtttgaaatt aaaaaaactc caagtagaga
    24241 aaaaatgtgg ggtaatggtt taaatggaaa ttgtaagaat gtaacatatg aagacaaaat
    24301 aaactgcgta acattaaaac aagatagatt tagtaattca ggtttagtag aatttgggtc
    24361 tttctgcaga tacttaacaa ctagagaatt agagttagcg cagacgctac cagttggata
    24421 tacaaaaggt ttgagtataa gacaagcaca aaatgtcata ggtgatggtt ggacaataga
    24481 tgtgatagca catattttat caaatattaa ctagttcttt atatataaat tattattgaa
    24541 gcggttagct atcatgatac ttgtagcagt aagttttata gctggtaggg tttatgagta
    24601 tagattgaat ctgaaagagt gtgaaaattg taataacaac tatcctgaaa aatgagaaag
    24661 aagtggtttt atgaataaaa gaataatttg caattggtgt ggtaaattat tttacatccc
    24721 aaaacagtct aaaaaaattt actgttgtaa aagatgtgag agaaaggcta agaaaagcaa
    24781 tagagaacag caaaattaat tttaaacaat aaaactatgg gggaatagca atgaataagt
    24841 ttcaaaaagc agtttctcaa atggtgaagc aagaggaaaa agaaaacttg ttgcaaggat
    24901 atgagaattg tatagtaggt agaagtatat caagttccat aagaagatat gtaaaagggt
    24961 ttgaaaagtt tggatatagt gtacatgagg tttatgaatt tataaacgat attaataagt
    25021 atgagtaact ttaagtgata aaggaagtat ttgattgagt aaatacaata ataagaaaat
    25081 tgtaatagat ggaattaaat ttgatagtaa agatgagtca gagtattatt tatatttaaa
    25141 agaaaaaaag gaaaatggag aaataaaaga ctttggactt caacaaaagt ttgaactaca
    25201 acctaaattt aaaaaagatg ggaaaagcta tagagctatt acatatacaa ttgattttgc
    25261 catatacaaa tggaatggtg aagtcgttta tatagatgtg aaagggtata gtacacagca
    25321 gggtgaactt agaaaaaagc tttttgacta taaatatcag gacaaaaaat tgatatggat
    25381 tgctaaaagc aaaaaatatg gggtagatgg ttggatagaa tatagtgaac ttaagaaaaa
    25441 gagaaaagaa aataagaaaa aggtagctta aataaatagg agtgatgtta tggcaagtaa
    25501 agttaaaaag gagtttttta tggcaactaa aaaacacctt gagaactaca aacaactaca
    25561 tattaatatt gaaagtctaa aacttcaaat aaaaaatctc aaagagttcc atttaggtga
    25621 ttttatgcaa ggtttaagct atgacagcat tcccataagt aagactaatt caataagtaa
    25681 tcaagttgaa aatgagttaa ttaatcttga agaaaagata atagaaaagc agatagaatt
    25741 atatgaaatg gaagcactaa aatatacaat agatgtatcc ataagtaatt taaaacctat
    25801 acataaacaa attataaggt ataggtatat tgaaggctta gaatggagtt taatagttga
    25861 taaagtatac ttagaagaaa gacaattaag agaaagagct aatcaagcca ttagttcaat
    25921 atcaatagcc ttatttggta aaaaagcatt aatagagcaa gaaccattat ttaagatgtt
    25981 agatttatag gcagttaata actgtctatt tttttgtgaa aaagatatat ggaagttatt
    26041 gacaatatca cgcatacgtg atattattga agtatagaaa ggaggcgaaa agaaagtggc
    26101 taagaaaata aaagagttca gaaaactcat aaaagaatta actgaactcg tactcgaagt
    26161 tggcacacta ttagcagtca tcaaaatggt aatagatagc ctacattagt aaaatgttaa
    26221 gagtggaagt tgcaccttcc ctcttaacta aattataaaa catagtcact aaaaatacaa
    26281 tgaataagta tagagagtta acatttgaat tattgaaact actgtatgac actattaaat
    26341 taattggagc atcaatattg ttatattatg ttattaactc aattttctag caatgattag
    26401 gaggtatttt atgaatcgat ttgaaggtat ttatagcttt gcagaagcaa caaaattatg
    26461 ggatttgaaa gattctacac ttagaaaagc agttgcgaca ggcaaattag ttgaaaatat
    26521 tgattgtaaa aagtttggtc gtgattgggt agtaactatt gaagcaatgg aaagagaata
    26581 tggaaaatta aataatataa tgaaagacct agattaaatt ctaggtcttt ttattgccgc
    26641 ttttctgccg attttacaat ttaaaatgtg agataatagt attgtggaaa tgaatatttc
    26701 tctctcaaaa ctaaatatat gtgggctagg ttaagggatt cgcctagctt atatgaacaa
    26761 actaggcagg gcatgaggat gctgtaagtt caattcttac tatgttcaaa cttattaata
    26821 cactatatgt agatatgctg gattaaaacg gaatttaatt caaatgtcta aaagagtggg
    26881 gcttggtaac ctcactcaat ttgcaaggac tggtgtgtaa tcttaggttc gattcctaaa
    26941 acttgctccc ttaaatataa tatgtatccc caaaaaaagg cttagattaa attctaagtc
    27001 tttttttaat aattaatgta gataataata tttattgcta aaatgatgta caaatatgat
    27061 aatataaatt acataaagat atataaatag agggggatta taaatttgtt aaatgaaaag
    27121 aatctaaaga acataaagaa tatattacaa atggtacaaa tgaaacacct taaagatttt
    27181 gaaaattggg gaattaataa acgaaaaaaa gaaactgcta taaatagctt gataaacata
    27241 atacaaaatg atggagaaaa actagattat tttaaggaat ggatatcaat ctttacaata
    27301 gatggtcata ataattatta tgtttttaat tacaatgatt ctgacttgga attggaagat
    27361 ttaaaaaaca tagtagaaga tatagtaagt gaaaatttat tggagattga tactagtgat
    27421 ttagaagaaa ctaaggtcat atataaaaaa aatgatgtca taaacaaaag attgattata
    27481 agatttattt ctccagcttt aattagctgt aaaaatgaaa atgagaatgg gaatatagaa
    27541 tctggattag aaaaaacttt ttattttgca actttatttc tagacttaag tttgaaacaa
    27601 ataatagttt ctataccaca tactgtaggt ataaaaagca tagataatat agaggctaaa
    27661 gcaagagatt tctcagaaat agcaaaatat tatgtatcaa aattagaaga tttgttaact
    27721 acttataata tacaaataga aaaatacaat gattggatat atgatgctac atatgagctt
    27781 gctgaggaag gcagtgctca taataatcct gagataaaca aaaaatatga agaaaatatt
    27841 gataaaatag atgaattcgc aaaaaagata gctattgctt ctggaataac tgataaggct
    27901 ataatagaaa atttcaaaga gggagtggga atactctatg agaatattct aatagacgaa
    27961 tttggtgtgg tagaggatga aaatcaatac agtacttttg tgcaaaatgg tgatggagta
    28021 aattcatact gtagagttgg gtctaaaaca gcaaccctaa aatcaggaaa gggacatgct
    28081 atagccaaga catctagaaa caatgaggat gtcaagaatt taggtgtcat gaaatcattc
    28141 aatggaactt tatccagatt tttaattgaa gtcttagata aagaattata tttaataaga
    28201 acagacactt ctaaatttat agaagagagg gtgatttatg atgttatacg aaaagttgga
    28261 gaatataagt atacttttag gaaatcagag taaagcaaaa tctataatag aagaattaga
    28321 gaaattaatg aagagtgata tcattacacc aagattgctg gccaggaaaa ctaaagttac
    28381 acttttagag gctacaagag ttcttgaatt tttagttaaa gaaaatgaat tagaattttt
    28441 tattgttgtt gaatgttcta atccagatag cgtatatgaa aatgaaatag gtcactataa
    28501 atacttcact tcgataaaag aattcaatgc tttttcaaaa ggacaagaat gcccaatatg
    28561 tggatgtggt tataaataca actttgaaga tacaaaaata ggctttagaa gattggagaa
    28621 gtgatacagt gagtctagat accttaaaag aatatctaag catatgtgat gataatttaa
    28681 tttgggagcc tttttatgat gacagtatgc tattagacga attctctaat tatataaaag
    28741 aaatagaatt atctaaggaa tggaataaat ctaaaaatca tgagaaagga aaaattttag
    28801 aacaattagt aagatttata atgtctagat ttacaatatt aagtgatata tctgtaaata
    28861 agtctacaac agataatgag ctagatgttt ttgttaagtt taatgacaac ataccaatac
    28921 cattcttatg taatataaaa tcaaaaatag tatgtgaatg taaaaactgg aaaagtagaa
    28981 aagtggatgt aggaatggta tctaaactag cagaaatatg caataaaaat aatgcgggat
    29041 tgggcatatt tatttcttta aatggattga caggtaaggg gtggcaatat gctgaaggaa
    29101 aaagaagaaa attatattta tcagataaga tgccaataat atcgtttaca gtagatgaaa
    29161 taacgaaatt aaagtataaa aggtataatc ttctgactat gataaaatgt aaaatgcaag
    29221 cattaataga tgagatggaa tttgctggag gagaattaag attattagaa agtgaacttg
    29281 gatttataga ctgtttaaat gaaaatatag aatctttgaa aatgttgggt ttaataaccg
    29341 aagttgaata taacaatatt aaagtaagaa tcaatcatag atatgatatt gtaaactgag
    29401 aactccaaac gagttctttt tttattccca aaacgacaaa caaacgaggt ggtggtgtgc
    29461 aagatgtgaa agaaaaagta aaacaagatt acctaaaagg tatgaaacaa aaggaaatat
    29521 cagcaaagta tgacattagt ttaaatactt taaagtcatg gataaaaaga tacaattggg
    29581 ctaatgagaa aaagaagggt gcacctaaaa ataaaagagg tgcacccata ggtaataaaa
    29641 atgccactgg tcctcctgga aataagaatg ctgaaaaatt tggtttcttc tcaaaatact
    29701 tacctgaaga aacccaagac ttaattaatg agataaagga taaagataaa tttgatattc
    29761 tttgggaaca gataacaata caatatgcag caataataag agcacaaaag ataatgtatg
    29821 ttaaagacaa ggaagaaatg attaaggaat taaagaaata tgaaagcaca gaaaatggtg
    29881 agaagataga gtatgaattt caatttgcat gggataggca agcatctttt cttaatgcac
    29941 agagtagagc aatgagtgaa cttagaagtt taattaaaca gtatgatgaa atgattcata
    30001 aggattggaa tttggctaca gaggagcaga aaacaagagt tgagaagttg aaatgtgaag
    30061 ttgataacct aagtaaagat gatattggag atgatgagtt gaaaataagt gtagattatg
    30121 gtgatagaaa tgatagttag aatagatttt aatccagatt tcaaggaagc caattttact
    30181 aaaaaaagat acagagcaat gaaaggttca gcagggagtg gaaaatctgt taatgtagca
    30241 caagactata tactaaagtt aggagataag aagtatcaag gagctaatct attagtagtt
    30301 agaaagtcag aagctacaca taagtattca acgtatgcag agcttacagg agctataaat
    30361 cgtatttatg gtaaacaagc tgataagtat tggaaaacta ctttaaatcc tttagaaatt
    30421 aagagtaaag ttactggtaa ctctataatt ttcagaggag ttaatgatgc aaaacaaaga
    30481 gaaaaattaa aatcaattaa cttctcgaaa ggaaaattaa catgggtttg gtgtgaagaa
    30541 gctacagaac ttatggaaag tgacatagac atactagatg accgtttaag aggtatttta
    30601 actaatccta acctatacta tcaaatgaca tttacattta atccagtctc agctactcat
    30661 tggataaaaa gaaagtattt tgactataaa aatgatgata tatttactca tcatagtact
    30721 tatctacaaa atagattcat agatgaggct tactacagaa gaatgcaaat gagaaaagag
    30781 caagacccag aagggtacaa agtctatggt cttggagaat ggggagaaac tggtggagca
    30841 atacttaaaa attatgttat acatgaattt cctacagaat ctgagtattt tgacaatatg
    30901 aggttatcac aagactttgg atttaaccat gcaaatgtag tacttagaat tggctttaag
    30961 gatggagagt tatatatatg taacgaaata tatgcacatg aaatggatac ctcagaaatc
    31021 ataaagattg caaatagtat aggtttagaa aagactctat ttatgtactg tgatagtgct
    31081 gaaccagata gaattaagat gtggaagagt gcaggatata aagctaaagg agttaaaaaa
    31141 ggaccaggaa gtgttaaagc tcaaatagat tatttgaaac aattaagaat acatgtacat
    31201 cctagttgca ctaataccat aaaagaaata caacaatgga aatggaaaca agatgaaaga
    31261 actggattat atcttgatga accagttgag tttatggatg atgcaatggc tgcgcttaga
    31321 tattctatag ataataagct taaaaataat ggaataagct tcttaaagta aaggaggtgt
    31381 taaatattta tataagtgaa acagatttaa taaaagttca gttaaaaaaa gagagcacca
    31441 ttaacctagt aaaagtcata gaacactaca tcttaaagca tagaccagaa aaatataaac
    31501 aaggagaaga atactattat ggtaatactg atgtaaacaa taagagaaga tattatctct
    31561 tagatggagc taaggttgat gattttacta aagttaataa taaagcaatt aacaactacc
    31621 ataagctttt agttgaccaa aaggtaggct atagtgtcgg aaatcccata gtatttaatg
    31681 cagatgatga taatctcact aagcttttaa atgacttact aggagaagag tttgacgata
    31741 caataacaga actatatctc aatgctagta ataaaggggt tgaatggtta catccatata
    31801 ttaatagaaa aggtgagttt aaatatgtaa taattccagc tgaagaagca attcctattt
    31861 gggatagtaa aagacagagg gaattagttg catttattag gttttattat attgaagata
    31921 tagatggaaa taaaataaaa agagttgagt actacacaga aaatgacata acttacttta
    31981 ttgaaagagg taatagtttt attcaagaat ttttatatga tgaatatgga aaaatgactg
    32041 atatacaaga aggtcatttt agaataaata acaaagaaca gggatggggt aaagttccat
    32101 ttataccttt taaaaataat gaaaagtgtg tctcagattt aactttctat aaatcattaa
    32161 tagatatata tgacaataat atttctacac tagcagataa cttagatgaa atacaagagg
    32221 ttatttatgt attaaaagaa tatccaggaa caagtctaca agagtttata gataatataa
    32281 gatactataa atcaattaaa gtagatggtg gaggtggagt tgataaacta gagataaata
    32341 taccagttga agctaaaaag gagcttcttg atagattgga aaagaatata attatctttg
    32401 gtcaaggagt taatccagaa tctcaaaaca caggtgacaa atcgggtgta gcacttaaat
    32461 ttttatattc actacttgac ttaaaatgtt ctaagactga aaagaagttt aaaaaagcaa
    32521 ttagagagct tttatggttt gtgtgtgagt atttaaagat aagtggtagt aagagctatg
    32581 attataaaac agttcaaatt acttttaatc actctatgat aataaatgaa gctgaaaaga
    32641 tagatatggc agctaaatca actggaattg tatcagatga aactattgtt tctaaccatc
    32701 cttgggtcga ggatgttaat gacgaacttg agagacttaa aaaacaggaa gatactcaaa
    32761 aagagtatga tgatttaatt cctaataatc aagatggtgt tatagatgaa acataaagat
    32821 tattggagaa agagatttga acaattagaa gaagctcaaa ataacaaaag tgtaaaatat
    32881 tatcttgaat tagaaaagca atataaacta gctataaata gtatagaaaa agatatatta
    32941 gcatggtaca acagatttgc caaaaatgaa ggaatatctt tattagaagc taagaaacta
    33001 ctaaatacaa gagaactaga agagtttaaa tggagtgttg aagaatatat taaatatggt
    33061 aaagaaaatg ctataaatca aaagtggatg aaagagttag aaaatgctag tgcaagagtt
    33121 catataacaa ggcttgaagc tttaaagtta caaatacagc aacaagtaga agttttatat
    33181 ggaaatgaac ttgatggtat tgataaacta atgagagata tttatacaag tggatactat
    33241 catacagctt ttaatgttca acaaggagta aacgttggtt ggagtttaat gagtcttgat
    33301 actaatagaa taaataaaat tatctctaaa ccatgggcaa tagatggatt aaactttagt
    33361 gaaagaattt ggggtaagta tagacctact ttagtaaatg aactacatac taagctaact
    33421 caatcaatta ttagaggtga aaatccaaaa aatttagtaa atgactttgc taagagattt
    33481 aatgtatcta aatcacaagc taagaatttg ataatgactg aatcagcttt ctttgcatca
    33541 gcaagtagaa aagattgttt tagtgattta gaagtagaga aatatgagat tattgctaca
    33601 ttagatttaa gaacttcaaa tatatgcaga gagttagatg gaaaaatatt tgatatgaaa
    33661 gattatcaag ttggaataac agctccacca tttcattgtc gttgtaggac aacaacagct
    33721 ccttggttcg aggatgaaga aggctataga gcagcaagag gagaagatgg aaaaacatat
    33781 tatgtaccat ctagtatgaa gtataatgag tggtatgaga agtatgttaa aaataatagt
    33841 aaacaaactg gtgcaaaata tactaaaggt gatatcgagt ggaatataag aagagaagaa
    33901 gaagcagaac tatattacga taatattaga aatagaaaag atgatatttc caaaatatca
    33961 aagaatacaa attggtcaga aaaaagtata ggtcaaatta aaaatcatat tttctacaat
    34021 actcatataa tgagagatgg aactagacgt atgttggatt ctgactatag tatgtcagtt
    34081 gcttggcaaa gacttataaa tggtacatac gaagatattg atattctctt attaaaacat
    34141 gaataccttg aaagtatatt tgagaaaaag tataatataa gtaacttaga agcccataga
    34201 atgactgaga aaaagcatga ttggtataaa gaattaatta aacagaaagg agagtttgaa
    34261 gaagatgatt gtcttaatga acttattaga aaagaataat gaatatgtta tatatagtta
    34321 tggatatgaa gaaaataagc ttgatggaag aataaaaata tatttagatg atttttataa
    34381 ttatgaaata ataaaagagt caaaagatga acatataagt aaatcagcaa cgttaaaagc
    34441 tatttctaaa cttataaaag ctgctaaaaa taacgatttg aaaaaagaaa tgagttatca
    34501 atgttagaag cacttactga acaataaatt agtaggtgct tttattatgt aaaagtttaa
    34561 aaaagtaggt gattttaatg tatatattaa ctcaagttat agctgtagtt tgtgtagtac
    34621 aaatctttat taattgtatt gctaatgtca atgtaggtat tctttgcaat aaattaaaag
    34681 aaaaaaatga agctaatata gataaagttt ctgatgaaat tctaaagaga gtaggagaag
    34741 aattaaatga atcactagac aaaagtcttt aaagactttt tttattgtgt aaaaaatgaa
    34801 aggagatatt taaaagatgg attggttaaa agaattgcta gaaggaataa aaatagaaaa
    34861 taacaaaatt gatgtagttt ctcttcaaaa atctatagaa aagaaaataa aagagactac
    34921 aattactcaa gaagattata caaatcttga aacacagctt aatacagcta atgaaactat
    34981 taaaaagttt gaaggaggta tgacaaaaga agatgtagag aatctaaaaa caacttatga
    35041 aactgataag aaaactttgg aagaaactta caaaaaagaa attgaagaaa aagattttaa
    35101 ttactggtta ggtgatgctt ttaaatctgt taaatgtaga gatgaaatag cattaaaagc
    35161 tcatttagat atagaagcac taagaaatag taaagacaga caaaaagctt ttgaagagca
    35221 aataaatcct ttgaaacagg ataaagatta tttgtttaat gcaacactag aaggtgaaga
    35281 acctaaaata gatactataa caccagggca agagcctaag ataaatgatt ttggttttaa
    35341 ttttactggg gtaagacctc atgaaaataa taataaatag gaggaaataa aatggcagca
    35401 ctaaattatg caaaagaata ttcaaatgtt ttagcacaag catatcctta tactttaaac
    35461 ttcggggatt tgtatgcaac accaaataat ggaagatata gatggactgg ttctaaaaca
    35521 atagaaatac caactatatc tacaactgga agagtagatt caaacagaga tacaatagca
    35581 gtagctcaaa gaaactatga taatgcttgg gaacctaagg tattaactaa tcaaaggaaa
    35641 tggtctacat tggttcatcc agcagatata aaccaaacta attatgtggc ttcaataggc
    35701 aatataacaa aagtatataa tgaggaacaa aagtttccag agatggatgc ttactgtata
    35761 tctaaaatat atgctgattg gaccgcatta ggtaacacag cagatacaac tgttcttaca
    35821 acagcaaacg tattagaagt atttgataag ttaatggaaa aaatgacaga agctagagta
    35881 cctgaaaatg gaagaatatt gtatgttact ccagtagtaa atacacttat caaaaatgca
    35941 aaagagatac aaagaacagt aaatataaag gatgcaggaa cttctcttaa tcgtcaaaca
    36001 actgatattg acacagttaa aataattaaa gtaccatcta atctaatgaa aactgcatat
    36061 gattttacaa ctggatggaa agtaggagca ggagctaaac aaatctttat gtccttagtt
    36121 cacccaagtg caataattac acctgtttct tatcagttct ctaagttaga cgaaccaaca
    36181 gcagttacag agggaaaatt cttctacttt gaagaaagtt ttgaggatgt atttatatta
    36241 aataaaaaag ctgatgcaat acaatttgtt gttgaagcat agaaaaagga gtgatatata
    36301 gtgccacaag taaaaaaact aaatagaata ctaaccatag aagaatgtaa aatagatgat
    36361 ttcttagaga tgggatatga tttgatagat gaaactggta aggcagtaaa gtatggcaag
    36421 tcattaagtg taaaagattt aatagctgaa aataatattt taaggtcaaa agttgagtct
    36481 ttagaagaag aaaataagca gcttaaagag aaaaataaac ttactaaaaa gtaggtgaaa
    36541 attatggaaa ataatataat tgatgaaata gaaaaaagac ttgaaagttt tggatatata
    36601 ttaaaagatg gagataagtg gttaataggt tttgtaagag aaaaaataga aaatattatt
    36661 aaactagatt gtaatataaa aactatgcca attgaattga aagaaattga agttgatatg
    36721 atagttggag agttcttatt taccaagaaa aatatgggtc aattagatat agaaagcatt
    36781 aactttgaag ctgtagaaaa gtctatatca gaaggtgata caaaggtaga ttttgctata
    36841 ggaagtggtt ctcaaacacc agaacaacgc tttgatagct taatagctta tcttactact
    36901 tatggcaaga ataagatatt aacctttagg tgcttaagat ggtaagtaaa actagaaaag
    36961 caatagaaat gttatataga gataaatgta ctatagttga gtatcagcca attaaagacc
    37021 ctgtaacaaa acgaactaac aataaagaag tgattgtatt agaaaatcaa ccatgtaaac
    37081 tttcatataa aaatatagtt tctgctacag aagggaaagt agctaagcta gagcaaacta
    37141 ttaaactctt tatatctcca gatatagaaa ttaaagcagg ttcaaaactt attataaatg
    37201 ataaagagta tgtaagaagt ggagaatcag ctatatatcc aaatcatcaa gaaataatac
    37261 ttgagttatt taaggataaa gcataatggc tagatggggc agtgttgatt ttagagagtt
    37321 taaaagagtt tgtaaaaaga tggaggagct tacaaagatt gatttagata agttttgcaa
    37381 ggatgcagca agagaattag cagcacgatt acttgggaaa gtaattagaa gaacaccagt
    37441 tgatacagga ttcttacgac aaggatggaa tggagtggct tatgctaggt cgcttcctgt
    37501 gtataaacaa ggaaataatt atattataga ggttgttaat ccgactgaat atgccttaac
    37561 acaatggggc atataaaacc cagcaaaatc ggtaaacgct aagtgtaata gtgttacatt
    37621 ataaagtagg tagaatatgg acatagaata actcttgatt tataatgtat aaaagaggtg
    37681 attttatggc aaaattttta gatattacag gaaaaaaatt tggaaggcta agagtcatta
    37741 aattttctaa agagataaaa agtggtaaaa gaaatagaaa atattggtta tgtaagtgtg
    37801 attgtggaaa tttcaaagaa ataagaactg actctttaac tagtggctta gtacaatctt
    37861 gtggatgttt aaaaaaagaa caagataaat taaatttaac agacaaatac caatttaaaa
    37921 agaaatataa ggttcaaaat aaaagacttt acagtatatg gaagggtata atatctagat
    37981 gtacagataa aaataataaa agatacaata gatatggtga aaggaatata attgtgtgtg
    38041 atgaatggtt ttgctatgat aattttgcaa attgggcatt gagtaatgga tattcagaga
    38101 aacttacaat tgatagaata aataatgaag gaaattatga atcaagcaat tgtagatggg
    38161 tggatataaa gacacaatgt agaaataggt caacaaatat attagtaaag catgaagaaa
    38221 aagaaattac attgatagag ctttctgaaa aaacaggtat ttcatattct tgcttaagaa
    38281 gtagatattc taagggatta gtaggaaata atcttataga aaaagttaag attattgaag
    38341 aaagcagagc taagttatct attgaggatg taaaagaaat tagaaaaaag tattctgatg
    38401 gatatacaat aaaacaacta agtgaaatat atcctgtaac atattcatct atatcaaata
    38461 tagtccatag aagaacatgg aaaaatattt aattaattat atatgccaat accgaggaaa
    38521 acctatagat tgcgaatagg ctataggttt ccgtagagcg tagagagtga ataaatataa
    38581 tctctccaag agtgctgggc aactaaataa agtttatttt gttgatgatg tacgccgaac
    38641 ttataggaaa ctataagagc tagaggataa aaagccttta ggataacaaa atgcatatgt
    38701 tgaatatggc catagaacta aagatggaaa aggttgggtt aaaggacaac atttcttaac
    38761 aatttcagag atggaactac aaagccaagt tgataagatt atagagaaaa aactattaat
    38821 attgcttaaa ggagtatttg atgcttaata atataattga tggaatatct attaaattag
    38881 ataaaacatt tggagagagt tatacaattt atagtgaaga tgtggagcaa ggtataaatg
    38941 aaccttgttt ttttattgtt cctttaaatc caagcaaagt atcctatcca agtggcagga
    39001 cattaaaaaa gaactctttt gatgtacatt attttccaaa aagtaatgat aaatcatttg
    39061 aaatagatga ggtagctgag atgctactgg aggaattaga gtatatagaa attgatggag
    39121 atttagtcag aggtacaaat atgaactttg aaattgtaga taatgtactt catttctttg
    39181 ttgattataa ctactttact ataaaaaata atgatatcaa taagatggat acagtagagt
    39241 tattcggtgg tttgaagaga ggtgataatt ttgagtaaga cattaagtaa aggaaccgat
    39301 tacaagttta ctaaggagca gatagttaat tctaagaagt atataaatag aaaagactta
    39361 ttaaatgcaa ttttaaaaga aaatgagtta tattccttct cagaggtaga ggaaataata
    39421 aatagcttta tgaaaggagt gagttaattt ggcgttaggt ggaggaacat ttgtaacaca
    39481 gaataaaata ttaccaggta gctatataaa ttttatctca gctaagaggg caaccagttc
    39541 attatcggat agaggtattg ttgcaatacc tttagagtta gattggggca tagatgaaga
    39601 cgtatttcaa gtaaccagtg atgattttga gaagtattca gtgaagtatt ttggatatga
    39661 ttatactcat gagaagctga aaggtttgag agatttattc aaaaatataa ggttgggata
    39721 tttttataaa ttaaataaag gcgttaaagc cagttgtact atagccacag caaaatatag
    39781 tggtatcaga ggaaatgact taaaagtaac agttacaaca aatatagatg ataatgctaa
    39841 gtttgatgtt gtaacacttt tagataataa gaaggtagat actcaaatag caaaggttat
    39901 tacagactta caagacaatg actatatcac ttggaagaag gatgcaacac tagaagcaag
    39961 tgcaggactt gtatttactg gtggaactaa tggcgaagct gtgacaggag cagagtacca
    40021 agctttcttg gataaaatag aaagctatag ctttaatgct ttaggatgtt tggctacaac
    40081 aacagaaatt aaaagtttat ttgtagaatt tacaaagaga atgagagata aggtaggagc
    40141 taagtttcaa acagtactat ataagaaaag tgatgcagat tatgaaggtg tagtgtctgt
    40201 agaaaataag attaaagata ttggattagt agaatctagt ttaatttatt gggcggctgg
    40261 agttatagca ggatgcgata taaataaatc taatactaat aaaaagtatg atggtgagtt
    40321 tgatgttgat gttaattata cacaaataca acttgaagaa gctttaaaaa ctggtaaatt
    40381 tatattccac aaggtgggag atgaagttca tgtgttagag gatataaata cttttgtatc
    40441 atttacagat gataaaaatg acgatttttc aagtaaccaa agtgttagag tacttgacca
    40501 aattgctaat gatattgcaa ctttatttaa tgaaaagtat ttaggtaaag ttccgaatga
    40561 taaggcagga agaataagtt tctggaatga tgttgttaaa caccataaag aattagagaa
    40621 tataagggca atagaagatt ttaaaactga tgatgttagt gtagagcttg gaaatgataa
    40681 gaaaactgtc atagtatctg atgctgttaa ggtaataaat gctatgagta agctttatat
    40741 gactgtttca gttaattaga gaggagagtg ataatatggc tcaaacaata aatgctaaag
    40801 atacagttag tgcaaagaaa gctgaatgtt ttataactat agaaggtaaa agatataatt
    40861 ttatgcaagc tatagattta gaggctaaaa tggaaaaaaa taaaagtgaa gttccaattc
    40921 taggaagaac aacaaaggga aataaaacaa ctgggagtac aaatactgga agtgcaacat
    40981 ttcattataa tacttctatt tttagagaat tactttacag atataaagaa actggtgagg
    41041 atatttattt tgacatacaa gttacaaatg aagaccctac atctgctgta ggaagacaga
    41101 cagtagtact taaagattgt aatatggaca gtggaataat tactaaattt gatgctgatg
    41161 gtgagtattt agatgaagat atggatttca cttttgagga ttgggaatta gtagaaaaat
    41221 ttaatttatt ggcaggaatg gagtaaaata cacatttata aattatatat gtgtattttt
    41281 tatatgaaaa attaaaataa aaggagatta gaataatatg agtaatttaa gtgctttttt
    41341 aagtcaaaat gcaataaagg ttgataatgt aaaatatgta gcgagtaaca gatttttaga
    41401 taaagaaggg aaaccagttg aatgggaatt aaaagtttta tcatctgaag aagacgaagc
    41461 actaagaaga aagtgtacta aaagagtaaa agtgattggt aacaatggta agcatactgg
    41521 acaatataca agtgaaattg actacaatag ttatgtagct gaattatgtg tagcatctac
    41581 agtatttcca gatttaaagg atgccgaact ccaaaatagt tatggagtaa tgggagaagc
    41641 tcagttatta aagacaatgc ttacagcagg tgagtatgtc aattatacag taaaagtgaa
    41701 tgaagtcaat ggatttgata catcttttga ggataaagta gaagaagcaa aaaactaatc
    41761 agaggtggcg attttgatgc tagcatcact cattattgta ttcaaaaatt aaagtggaag
    41821 ccaagtgaat atatgaattt agaagttaat gagagagcgt tagcagccgc ctcaatactt
    41881 ataaagatag aagatgaaga ggaagcaatg aaagaagctg aaagagagag aaagagggga
    41941 cgaagaagat agcaaaataa aaaaataaat atagaatagg taaaatatgt aataattata
    42001 tgttataata tttttagcaa gaagatgtaa tctacaattt atagagtgga gttcatactg
    42061 ggataaaacc tacttcctaa tgaaaggagg tgggaagtat gaataacttt ttacttaatg
    42121 taatagctgg cgttattgct agtttaatat tttgcttaat ttgtaaagta tttctaaaag
    42181 taaaaagcca ctcaactcgt ggcaagagta aaagtggctg ggaatttgat tttaaaatca
    42241 agttccataa gttcaaatag attcatttaa ttatgaactt cactctaccg caaaatagat
    42301 tgtagttctt cttgctttta ttataccaca aattagaaaa aatattgttt atataaaata
    42361 aaaaataaaa atttttatta aaaaattgaa aacttgatta taaagcaatt aatttataaa
    42421 atatatataa ataagtaggt atttatttac ttgaatttca ttg
//
LOCUS       DBGJFU010000020        42021 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.020, whole genome shotgun sequence.
ACCESSION   DBGJFU010000020 DBGJFU010000000
VERSION     DBGJFU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.020"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            199..1047
                     /locus_tag="ACOIAS_001051"
     CDS             199..1047
                     /locus_tag="ACOIAS_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGZ2700770.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1111..3033
                     /locus_tag="ACOIAS_001052"
     CDS             1111..3033
                     /locus_tag="ACOIAS_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2700771.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3115..3978
                     /gene="aroE"
                     /locus_tag="ACOIAS_001053"
     CDS             3115..3978
                     /gene="aroE"
                     /locus_tag="ACOIAS_001053"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGZ2700772.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4178..5395
                     /locus_tag="ACOIAS_001054"
     CDS             4178..5395
                     /locus_tag="ACOIAS_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2700773.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5631..7496
                     /locus_tag="ACOIAS_001055"
     CDS             5631..7496
                     /locus_tag="ACOIAS_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HGZ2700774.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7674..9038)
                     /locus_tag="ACOIAS_001056"
     CDS             complement(7674..9038)
                     /locus_tag="ACOIAS_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HGZ2700775.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9217..10560)
                     /locus_tag="ACOIAS_001057"
     CDS             complement(9217..10560)
                     /locus_tag="ACOIAS_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGZ2700776.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10633..10734)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11063..11716
                     /locus_tag="ACOIAS_001058"
     CDS             11063..11716
                     /locus_tag="ACOIAS_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGZ2700777.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            11857..13128
                     /gene="brnQ"
                     /locus_tag="ACOIAS_001059"
     CDS             11857..13128
                     /gene="brnQ"
                     /locus_tag="ACOIAS_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGZ2700778.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            13330..14517
                     /locus_tag="ACOIAS_001060"
     CDS             13330..14517
                     /locus_tag="ACOIAS_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HGZ2700779.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(14747..16771)
                     /locus_tag="ACOIAS_001061"
     CDS             complement(14747..16771)
                     /locus_tag="ACOIAS_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2700780.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            17161..17829
                     /locus_tag="ACOIAS_001062"
     CDS             17161..17829
                     /locus_tag="ACOIAS_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGZ2700781.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            17831..18316
                     /locus_tag="ACOIAS_001063"
     CDS             17831..18316
                     /locus_tag="ACOIAS_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGZ2700782.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            18377..19498
                     /locus_tag="ACOIAS_001064"
     CDS             18377..19498
                     /locus_tag="ACOIAS_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HGZ2700783.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            19779..20969
                     /locus_tag="ACOIAS_001065"
     CDS             19779..20969
                     /locus_tag="ACOIAS_001065"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2700784.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    21103..21358
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21417..22418
                     /gene="asnA"
                     /locus_tag="ACOIAS_001066"
     CDS             21417..22418
                     /gene="asnA"
                     /locus_tag="ACOIAS_001066"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGZ2700785.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            22522..22911
                     /locus_tag="ACOIAS_001067"
     CDS             22522..22911
                     /locus_tag="ACOIAS_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HGZ2700786.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            23072..24280
                     /locus_tag="ACOIAS_001068"
     CDS             23072..24280
                     /locus_tag="ACOIAS_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HGZ2700787.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            24656..27700
                     /locus_tag="ACOIAS_001069"
     CDS             24656..27700
                     /locus_tag="ACOIAS_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2700788.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKIRTDASTRMSADQISDVA
                     LSIEYVKQYTDSHSGISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGARAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     VYPLTIKAGETATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTVAETDSDGATHYITKLADAYKVSGDSFYMQGVKAVLPL
                     GTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYVTQITEDGDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIIESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLEKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADEEEMKVEISYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENIELLINGKRVESDYTFTADSETMKVEVVFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPPTPEKPS
                     RPSDSPKTGDSTNVMAFVVMLLASAGGLAGIFFYKRRKMKNS"
     gene            27935..28261
                     /locus_tag="ACOIAS_001070"
     CDS             27935..28261
                     /locus_tag="ACOIAS_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2700789.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            28277..28660
                     /locus_tag="ACOIAS_001071"
     CDS             28277..28660
                     /locus_tag="ACOIAS_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2700790.1"
                     /translation="MRLSNGFVIDREKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGSPQNNNSQGQQKK"
     gene            28744..29382
                     /locus_tag="ACOIAS_001072"
     CDS             28744..29382
                     /locus_tag="ACOIAS_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2700791.1"
                     /translation="MKYIFLHGLGQTSFSWNKTLEVMSDKWDILCPNLADWLYNERPC
                     YETLYKALERCCKQFNEPLTLCGLSLGGILAMQYSIEHSEKVHSLVLIGTQYAMPKKL
                     LKMQNIMFQFMPSSMFSKMGFQKADFISLCKSMIDLDFTHNLKDISCRVLVICGEKDK
                     PNKSASLQLGNLIPYAKITIISGAGHEVNIDAPIKLGNELNVFFNSSHNTER"
     gene            29526..30317
                     /locus_tag="ACOIAS_001073"
     CDS             29526..30317
                     /locus_tag="ACOIAS_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700792.1"
                     /translation="MTLGEKIQKLRKQNGFSQEELAEKVTVTRQTISKWELNQSEPDL
                     DFIAQLSNIFNVSADYLIKEELTKPDELPFRKKRYQYYFSERSKRTMFVAISIVALIA
                     SIVCLICDYFTSDKLSWSFIAIEAIIAVWLVFLPCMLFKEKVIFKTLIICSIIPIPFL
                     AILALLLKVTTIFTLGSCISVLCIAIMWAIYGIFRKYHQRIFLSLGFSLLLLIFVPIV
                     IVRVTDYFLPQYEIVSKSDVFNGIITFAIALICFGIDYLTQHKNK"
     gene            30387..31769
                     /locus_tag="ACOIAS_001074"
     CDS             30387..31769
                     /locus_tag="ACOIAS_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HGZ2700793.1"
                     /translation="MRKIFNKGHRIRASDKHLVYHYCIGTLLFVFMAVLLLLNSKQLM
                     RTNWEHFSLLQNGLILSPYNFITILIATGVCVLVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHICCEITLGKYQDQ
                     LLRLEDKFESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNSDLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGRLAQERQ
                     VMPEQQGKEN"
     gene            31773..32189
                     /locus_tag="ACOIAS_001075"
     CDS             31773..32189
                     /locus_tag="ACOIAS_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700794.1"
                     /translation="MEIKEINKIIQSEDKDEKVKSVKCICINYGDFLADCEGFVQKRY
                     HDFKCNPKHQFEKKADTILENAIHEKNFMPDLFLIRLNRKQSACNSQIDFVFELLDKS
                     FLETDPIRKSEISEETLNLCLSADVSFIMVYIGMNR"
     gene            32434..33630
                     /gene="mobT"
                     /locus_tag="ACOIAS_001076"
     CDS             32434..33630
                     /gene="mobT"
                     /locus_tag="ACOIAS_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HGZ2700795.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLALASGITREYLNKIESGK
                     MKPSKELLNILHEELARFNPEAPLTMLFDYVKIRFSTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSSDEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMDTIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            33643..33777
                     /locus_tag="ACOIAS_001077"
     CDS             33643..33777
                     /locus_tag="ACOIAS_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HGZ2700796.1"
                     /translation="MWVLLKDFLLVSMGMGIGVVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            33778..33999
                     /locus_tag="ACOIAS_001078"
     CDS             33778..33999
                     /locus_tag="ACOIAS_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700797.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            34070..34696
                     /locus_tag="ACOIAS_001079"
     CDS             34070..34696
                     /locus_tag="ACOIAS_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2700798.1"
                     /translation="MKYIMLHGLGQSSESWSDTVKVFNDDFEVLCPNLSDWLSGKTPC
                     YDTLYRTLETYCEQFEEPLNICGLSLGGILAMQYAIEHPEKVNSLVLIGTQYEMPKYL
                     LKMQNLIFRIMPCSAFEKMGFRKTDFINLCKSMMELNFKHELKKISCSVLVVCGDKDK
                     INKKASLELQQQIENAEIAIIENAGHEVNTDNPTLLGQKLNVFFKQYV"
     gene            34785..35081
                     /locus_tag="ACOIAS_001080"
     CDS             34785..35081
                     /locus_tag="ACOIAS_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2700799.1"
                     /translation="MNDIFKDMQSKVGCDYISDLPSYKRKVWHEMKRLNLADYEERQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLNCR"
     gene            35000..35503
                     /locus_tag="ACOIAS_001081"
     CDS             35000..35503
                     /locus_tag="ACOIAS_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2700800.1"
                     /translation="MQETRVLVETRGTTGEETISYWFELPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            35521..36024
                     /locus_tag="ACOIAS_001082"
     CDS             35521..36024
                     /locus_tag="ACOIAS_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2700801.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHFGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            36141..36539
                     /locus_tag="ACOIAS_001083"
     CDS             36141..36539
                     /locus_tag="ACOIAS_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2700802.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            36517..38967
                     /locus_tag="ACOIAS_001084"
     CDS             36517..38967
                     /locus_tag="ACOIAS_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2700803.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQAVILDPKSERGNWKE
                     TLPEIAREINIVNLTSDKDNAGLLDPFVIMKDKEDGATLAKEILTFLTGISTRDGDKF
                     PVLISAISKVSESEHRGLLNVITELRQENTPIANHIANHIASFTNYDFAHLLFSDGTV
                     ENTISLDNQLNIIQVADLVLPDKDTTFNEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            38967..41129
                     /locus_tag="ACOIAS_001085"
     CDS             38967..41129
                     /locus_tag="ACOIAS_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HGZ2700804.1"
                     /translation="MKERIKGAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINVENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTNRVEALV
                     SASPEVEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYLAHRARRMERRLAGAVTAGGVAGAVAGSTISNRKPTRNTATKDSRGNTSTSSMGQ
                     RAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKRGMVQ
                     EQQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATA
                     SKPSAEKMQEVKRPAIAQTPKASEPVKTNVIKERPLSSGASDKKATQSAQITHRQNVE
                     KVVSQETRQNYTKDRRTKVQQTTEKTRNLVTKKGQKKK"
     gene            41126..>42021
                     /locus_tag="ACOIAS_001086"
     CDS             41126..>42021
                     /locus_tag="ACOIAS_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HGZ2700805.1"
                     /translation="MKLKHIALIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKEKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVG"
BASE COUNT        14636 a         6142 c         8261 g        12982 t
ORIGIN      
        1 atctatgaat taataggaaa ttatcaattt gttattagta aagtctcgtg atacaataaa
       61 tttataaaat ctagataaat agaaatcaaa aaatactttt atttttaagg taaaataata
      121 ctactagtag gataaataaa aatataaaat caaaaatact attaattata aagaaaataa
      181 agggggcaaa tttaagctat gaataatcaa aaaaatctta atattccaat tacaattagt
      241 tcttatacat tgggaacaga agtttcattc cgtgatagag ttcgtattgc aaaagaagct
      301 gggtttgatg gtattggatt gcgtgctgaa aattatattg atgcaaaaaa tgcaggtgtt
      361 acagatgaag aaatgattgc aattcttaat gaatatgata tgaaagttac agaagtagaa
      421 tatataactc aatggggaag agaagaagat agaacggaag ctcaaagaga aaaagagcaa
      481 aatgtttatc atatggctca cttatttaat gttaaacata taaattgtgg attattagag
      541 atgataccag aagaacaaat tataactgca ttgggagaat tatgtgacag agcaggtgaa
      601 cttattatag gtcttgagtt tatgccatat agtggtgtac cagatttggc tactgcatgg
      661 cgtgttgtga aaggatgtaa tcgtgaaaat gcaatgctta tattagatac ttggcactgg
      721 gctagagcaa agcagacagc agaggacctt gcacctgttc cagcagataa gatagtatca
      781 attcaagtgt gtgatgtttt agaaactcct tatgaaaaac ttagggatga atcactacat
      841 gaccgtttgg ctccaggtga aggatatgga aacacagagg aatttgtgaa aataataaaa
      901 gaacacaata tatcaccaag agtcataggg gcagaagtaa tatctgattc tctaatatct
      961 aaagggttat ccactgctgc tgaaactgta tttaatgcaa ctaaaaaagt tttggataaa
     1021 gcatggtctg aagtatctcc aaaataaact tatataatat aaaaatatac agagtattat
     1081 aatataaaac ttgaaaggaa ggaaaacctt atgaagatta gttctatgtt tacacctata
     1141 agaattggtt caatgacagt tccaaatcgt tttgtagttc cccctatggg aaacaatttt
     1201 gcaaacacag atggtacttt aagtgaaact tctaaggctt attatcttga gcgtgcttta
     1261 ggtggttttg gattaataac tatagaatct agtgttgtag acaaaaaagc aaaaggaggt
     1321 cctcgtaagc cttgtttata tgatgatagc acaatagata gttttaaaaa tgtaatagat
     1381 gcttgtcatg atgcaggttc gaaggtatct atacaattac aacatgctgg ttctgaagga
     1441 aatgaaaaag tagcaggaca tccattaaaa gctgcttctg caatacctgc atcaaatggt
     1501 cgtaatacac cactagcaat aacaacagaa gaaatatatg aacttataga atcttatgga
     1561 gatgctgcat tacgtgcaca aaaagctggt gcagatgctg ttgaagttca ctgcgctcat
     1621 ggatatctgg ttagtagttt tatatcacaa agaacaaata agcgtgtaga tgagtttggt
     1681 ggatgttttg aaaatagaat gagattacct cgtcttatca ttgaaaatat acgtaaaaaa
     1741 gtaggaaatt ctctagcaat attatgtcgt ataaacagca ctgatgatgt tccaggagga
     1801 attgatgtac acgatagttc agtaatagca gcatatctag aagattgtgg aatagatgga
     1861 cttcatgttt cacgtagtat acatatacat gatgaatata tgtgggctcc tacgacttta
     1921 catgctggat tcagtgcaga attagttact gaaataaaaa aagctgttag tataccagta
     1981 ataacagttg gacgttatac agagccacag tttgctgagt taatggtcag acaaggaaga
     2041 tgtgatttag tagcattcgg tagacaaagc ttggctgacc cagaaatgcc taataaagct
     2101 aaaaatggaa aactagacga aatgatacca tgtattgcat gcttacaagg atgtgtacca
     2161 aatatgttcc aaggcaaacc tatagcctgt cttgcaaatc ctatattagg acatgaagca
     2221 gagcttaagc cagctgaaat tagcaaagaa gttcttgttg ttggtggagg tgttggaggt
     2281 atgcttgcag catgggtatg tgctaaaaga ggacataatg taactttagt tgaaaaatct
     2341 gaagttcttg gtgggcaaat gcgtttagct gcatatcctc cagggaaagg agatattact
     2401 aacttagtta gaagttatat ttcaaaatgt aatcaatatg gtgtaaaaat atgtactaat
     2461 acagaagcta cagtagaact tataaaggag aaatcacctg atgtagttat tatagctact
     2521 ggagcaactc cacttgtact tcctatacca ggaatcaatg attcaggatt aattcatgct
     2581 gtagaccttc ttgatggtaa aaaatcttgt gggaaaaaag tacttgttgt tggaggtgga
     2641 atggttggat gtgaagtggc agccttcctt ggagaacaag aacatgaggt tactgttatt
     2701 gagcttagag aagaagttgg tgcagatgta atttcagaac accgtaaatt ccttatgaat
     2761 gattttgaaa attataatat acataccatt actggtgcaa aagtttctaa attttatgat
     2821 gatggtgtcg actatttact tacagatggc acagagcatg atttaaaagg ttttgattca
     2881 gtagtacttg ctatgggttc tcgtaattac gacccattaa gtgaagttat aaaagaagtt
     2941 gtaaaagaaa cttatattgt tggtgatgca gtaaaagctc gtagggcatt agatgcaaca
     3001 aaagaagctt ttgaagtagc attaaatata taaataataa ataaattttt ctttgtaaat
     3061 aactgaatag gttatttgca aagaaaatga taatattgag aggagattat agttatggaa
     3121 atttcaggta gaacaggatt atttgcatta ataggtacac cggttggtca ctctaaatca
     3181 ccagtgatgt ataattatag ttttaagaaa ttagatttag attatagata tttagccttt
     3241 gatatcacag tagataaggt aaaagaggca cttttagcaa taaaaacttt taatataaaa
     3301 ggagccaatg ttactatgcc atgtaaatct gctgttacag aatatatgga tgaactttct
     3361 ccagcagctc gtataattgg agcatgtaat acaattgtaa atgacaatgg aaagttagtt
     3421 ggtcatataa cagatggagt aggctatgtt cgtaatttaa aagaaaatgg ggtagaagtt
     3481 aaaggtaaaa agattacaat tatgggtgct ggtggtgcag caactgctat acaagtacaa
     3541 tgtgctttag atggagcaag agaaatatct atctttaatc ctaaggatga tttttataaa
     3601 agagcagagc aaacagttga gaatataaaa aaagatgtac cagaatgtgt tgttaatcta
     3661 tatgatttag aagatacaaa caaactttat gaggagatac aatcaagtga tatattgaca
     3721 aatgcaactc ttattggtat gaagccttat gacaatgaaa caaatataaa agatactagt
     3781 gttttaagaa aagatttagt agtaacagat gtcgtttata atcctaagaa aacaaaaatg
     3841 attgaagatg ctgaagctaa tggatgtaag gctattggtg gattaggaat gttattatac
     3901 caaggagcag aagcttttaa tctatatact ggattagaaa tgcctgtaga agaagttaat
     3961 gaattatgct ttaaatagat aagagtaata tcttaaacaa tgaaaaaaca tacgtatttc
     4021 aatattttta ataataaatt acaaattgtc atagtgggtt ttgcctcata gtccagttaa
     4081 atttgaagtt ttaactgact atactttaaa tccattatgg ggatgagttt attgtacaca
     4141 aaaatgttaa taaattaaca agaatgggag aaaaattatg aacaataaaa aatattatcc
     4201 tactgccata gcgttgtatt tttcatattt tttacttggt attggaattt caattttagg
     4261 acaatacaaa ccagaatttt catcaatgtg gggggctaaa acattatcag atggaacatt
     4321 ggatgtaagt attgttttag ctgttattgc tgcactagga ttagggagac ttatatctta
     4381 tccatttgca ggacctattt cagataagta tggaagaaaa gtaagtggtc ttataggaaa
     4441 ctttctacat gcaattttct ttgttggaat tgtattttca ccaaattttt atatagcata
     4501 tgtatttgca ataattggag gggcagcaaa ttcattctta gatacctgtg taactccttc
     4561 ttgtatggag atatttgcta gtttaggaac aatagcaaat atgtttacta aatttacaat
     4621 agctttagca caatttttgc ttccttttat aattggattt gttgcagcaa attcaatatc
     4681 atttaaagtt atttttatag taacagcgat tcttattgta gtagatgcct ttttgatagc
     4741 tgtgttacca tttccaccag ctaataatgt tattgataac aaaggcaaaa cagttaaatc
     4801 agagaagatg aaatttacac caacaagtat agctcttgtt tgtattggat ttacatgtac
     4861 atctacattt gtattatggt taaattgtaa tcaggaattg ggtaaattgt atgggatggc
     4921 tgaccctaca aaaattcaat ccttttactc aatgggagta atatgtgcag tattaataac
     4981 ttctttactt attaaaaaat atattaaacc aataagaata ttagttatat atcctataat
     5041 agctttactt atgctattag ttgtatattt tgttcaaact ccaactattt gtatgttagg
     5101 gggatttgta ataggttatt cagcagcagg aggtgttctt cagttaacaa cgtcaactgc
     5161 aaatgaaatg tttccaacta ataaaggaaa aataacatct atagttatga ttgcatctag
     5221 tatagcaaac tatgttattt taaatatagc aggtattatt acaaaatcag ggggagttaa
     5281 tggaccaaaa tatgttgtat tgtttaatgt ggcaataaca tttgttggaa ttttacttgc
     5341 attgtttgta aacatgagat atgaaaaaga aaaagtgtat gattatgatg tctaatatta
     5401 gtataatata tgcatagaga agtctaaatc taggatagat ttttattaaa agtaattaaa
     5461 attaataagg atattattta cagttataag atagggtatt tacataatta ataataagga
     5521 tattactaga atttcaattt ctagtaatat ttttttgcgt ataaatgtaa acttgatgaa
     5581 ataagtagta taatcaatat aaattacatc atgtaaagga ggataatttg gtggagaatg
     5641 tttttaagcg attgcaggaa tttaatggtt atgatggata caaagaatca tttgaaatga
     5701 attacctatg tatatatgaa agtataccgt taagagaaca agtagagctt gcaaataatc
     5761 tagtagatga aattttaaat atgtataaaa gtgaatctaa tgaaatttat ttattggagg
     5821 gtagtaattc taagtcttta atttgttatt tcgaaatttt tatgaaaaag ataaatactt
     5881 tggtaaaaga aatgattatt gatgagaaat ggttatataa attaactaaa gaattaatat
     5941 ataagagtaa aaaagttgag tatgtaaaac tagggttagt tttaagtgag aaatatctaa
     6001 atgtagaaaa cttaagagaa gttgtagata cattttctaa atctggagaa tacgtatttt
     6061 atctgagtaa tactattaaa aaacttgaat tttacaatac atatttattt aatttatcta
     6121 aaaaggctac aggttcaata aaagtatttg ctattgttaa tatggaaaat ttagattcta
     6181 aaataaattc ctatctaatt gaagatggtt ataaggatac aaagtacgaa cgattattga
     6241 tgaactatat tatttcaatt gtagatttga atgaatatct tgaaaaacga gatttagata
     6301 aagaaaagat aaataattta gcacgtctta tatgcaatta cttgctaagt gtagaattta
     6361 aatatatagg taataaatta gaattagtga atcgattttt acctactgta gtaaattatg
     6421 ggactaactt tgaaagttta tattcaattt tcctaatagc tataaacgtc ttaaaagatg
     6481 aaaatataga gtgcaataag attgaatttg aaaaagaaat aaatggtatc ttgctcagtg
     6541 aaaaatggaa aaatatatat tttgaagcat taagagatgc tagtggaaaa acagaagata
     6601 ttataaaaat gtcagaaatt tatgatgtaa acttatcttt tgatgacttg ttgccatatc
     6661 taaatagaga tattagagat tttgaggtat attggcatat atctaaaaag ggaacgacaa
     6721 gttctagatt aaaattatta aatttttttg aagagacgtt taaaatagat gatttaattg
     6781 gaaaaatgaa agatatagaa aaagataaat tgactcaaga atattatgat gatatgttat
     6841 tttttattgt gcttaaagga agtaaatcac tatatcctga aggaaagaat atatctttaa
     6901 aaggaatatt tggaaatata aatgaagtaa gaaaagaatc cataaatatc ttaaaaagat
     6961 acagagagaa actatctctt gaagaattaa aaattgttaa agaagcgtat gaaaaagaaa
     7021 aaaatgttat attgaaggat gaattaagaa gagtattata tgaaagtaat aatttaaaaa
     7081 aagaatttgt aaatattgaa aaaataaaag tagatgaaca tggtaaggat atctatttga
     7141 cttcaatagc tgttgctggt tcaaggttta gaaatagaga atatcttgaa aaagaattgg
     7201 agaaaagtaa gatatactat ttaactagag agaaagataa tctatatgat gaaaaagcta
     7261 ttaaaatagt tggtgaaact ggatatgtga taggctatgt cccaagaaaa gaaaactata
     7321 ttttgagcaa tttattagat ggaggaaaac tgctgtattg tagagtaact gaatataatt
     7381 tatatgaaga ttgtatctac gcaaatgtgt acttgagtta taaagatgta attgaaacag
     7441 ttgaaaactc tttaaagatg gttttagata agagtagaat aaaacttata aactaagacg
     7501 tttatatatt gcttagatga tgtaatccta actgattggt tattttttct ggaattatgg
     7561 tgtattttat gagcaatgct aatgaatata aaataaaaaa acaattgttt tgtatatatg
     7621 atgtatataa atatatttat atattcatat ttacaaaaca attgaaattt atgttatttt
     7681 ataattttaa atttagatgg aattttaact tctgctttat cttttatttt agaagcttgt
     7741 tttttaactt tatttaataa ttcagattca tctacaacta atacttttct ttccttcatc
     7801 aaccatttac catcacaaat agtagagtct acattttcac tagtcataga ataaactata
     7861 tttccaattg gattatgcat tggtaaacaa tgaattgtat ctggatttag aataatcata
     7921 tcagcttttt ttcctacttc aagactacca atttcttttt ctagtaaagc acattttgct
     7981 ccatttatag tagccatttc taagacttct tcagctggta cgacagttgg gtttaaagtt
     8041 cttcctttat gtattagtga tgtaagatac atgtccctca taaggtccat tctattattt
     8101 gaaggagctc catcagtacc aattgaaaca ggtattcctt tttctaacat ttcaggaatt
     8161 gatgcaaacc ctagaaccac tttcatagct gaacctgggt catgagaaac ttttacatca
     8221 tgtaatctaa ataaatcaat ttccttatga gtaagccaaa ctgtatgagc agctaaaaga
     8281 tttggtccta atacacctag tttatcaagg tattcaactg ttccaactcc attgttttgc
     8341 tttacaaatg aaatttcatc agctatttca gcaatatgca tatgtatacc tgtattgtat
     8401 ttatcagcta gttctttagt tctaacaagt aattcttcag ttgtattaaa aatagttctt
     8461 aatgcaaacc atattttaat tctctcatta gatgtattat taaacttatg gaataagtct
     8521 aattgagtgt tgatttcctc atcagtagtt ttttgccatg cctcaggaag accatctcct
     8581 gtatccataa ctgatttagc taaacaggct ctaagtccag ttttatcaac agcttcaacc
     8641 atagcatcta catattgacc accagattct aaaaatgtag tgacaccaga ttttatcaat
     8701 tcaacacaac atgcagtaga tgaaatcaag gaatcttcat aattgaagct actttcgtat
     8761 ggccaaattc tttctctaag ccaagttaaa agtacaacat catctgcaag accacgtcct
     8821 aattgttgag ataagtgaac atgagtattg actagccctg gcattagtat ttttccttta
     8881 gcatcatata tctctgcatc tttatctaca atattaatat caaacttacc aacctctttt
     8941 ataatattat tttcaataag tatgcttcca ttttcatata cctctctatc tgcattcata
     9001 gtaattagat atgcattttt tataagtaat tgtgacataa acaccactcc ttttctatat
     9061 atttttatat ctatatatta tgataataaa ttttctatac aaagttaata gcaaaatcaa
     9121 tttacatata aagtgatttt gctatttata aatttaaaat aaataagatt ttattctata
     9181 taatttaaaa tattgaccta gaactattta aaaatattaa cttagaacta agtttacaac
     9241 tgcaagtaaa cctataatcc aaagtgtaat actgatttta tcagtttctt tagctactac
     9301 atgcattatt atatatgaaa taaatccaaa gcataaacca atacttatac tataagttaa
     9361 aggcatcaat aaaactgtaa caaaagcagg tacagctatt ttgctatcag caaaatcaat
     9421 ctgttttatg ctttcaaaca tgaatacacc aaccattaca agtgcaggag ctgcagcgta
     9481 agtaggaact ataccaagta atggtgtaaa tataagtgta gctaagaata atgctgctgt
     9541 tactattgaa gcaagtcctg ttcttgcacc tgctgcaata ccagaagcag attctccaaa
     9601 tgttgtaaca gtactagttc ctaagaatga gcctattaaa gtagaagaaa catctgcata
     9661 taacattctt cctaaatctt ttatttcacc attctcatct tcaagaccca tttgtttaca
     9721 acaagtaata agaaatgcta atgaatcaaa catgtctata aacatgaaag agaatatagg
     9781 tccaattaat gagaatttca atgcacctac aatatctaat ttaaaagcaa ctggtgctat
     9841 actaggaggt gtcgagatta ttgctgttgg caactccaca aatccaaata taacagatat
     9901 tattgttgca actactatac ttatcaatat tccaccttta acttttttat attctagtat
     9961 tgccattaag ataagagcaa aaagagctat taaaactgtt ggtgtaaaat ctgcaagtga
    10021 aactatagta tctggattag ctattataac acccatattt tttaaaccaa caaaagcaag
    10081 gaaaagacct atacctgctg aagatgctac tgccaattct ctaggaattg cgtctgcaat
    10141 tgtctttcga agacctgtta ctgcgagtgt aaagaagaca catccagata gaaaaactac
    10201 cccaagacct gtttgccatg ggattcctgc acctataacc aaagagtatg tgaagaatgc
    10261 atttaatccc ataccaggag ctagagcaaa tggtgcattt cctaaaatcg caaatataag
    10321 tgtaccaatg gctgtagcta gtatggtaac tgttataagg gcggctttat ccattccagc
    10381 ttcagataat attgctggat ttacgaaaac tatgtatgcc attgttaaaa atgtagtaac
    10441 tccacctatt acctctgttt taaggctaga gccacgttcg ctaaatttaa acattttgtc
    10501 taaaaagctt aatgatgaat tatttccacc tggttgtaca ttgttagtgt ttggtaccat
    10561 ttttacctcc aaaaaacttt aagatataaa caaaaaaata tatattcata tagatagaat
    10621 agaacatata ttcagagttg acattctatt catagaaaac ccatttaagg tgagtttgta
    10681 gaaactatta gaccgtatta ctaatattat atgaacattt tctatttgtt tttctgttgt
    10741 attatatcgt aatattgtat tatttacaat aaatataata aaattattcg tttttttgtc
    10801 gattttataa aaaattaagg ttatttattg aaaaaaatta ctttttccaa taattgaata
    10861 tgtatgaata taatgtcaat tattctaact tgttttatac tattaatgga agtttattat
    10921 aaatgagttt gtgatataat tataaaagta ctgaatacat ggatatttcg taaaaacttt
    10981 ttaatatatc aaatatgtat attaagttaa tatattaaaa aaaataatat caaaaataat
    11041 ataatttata gtggagggga aaatgactaa aattattaaa gtgcaaaaac ctaaatttac
    11101 aggagaatta gaaattattg aaaatcttga agatatactt gaagatatat ttaatgatga
    11161 aaaaatattt aataaaatta tagaaaatga agttataagt gatttagatt ctgtaagagt
    11221 tagctttgaa tcttgtatat ttagaaatgt gtcttttgaa aattgtaatt taaaaaagat
    11281 tgatttactt gatgtggtat ttgaaaactg caatttatca aatacatttt ttgatggagg
    11341 aagtatatac agagttgaat ttaaaaatag taaattgata ggagttagat ttgatgattg
    11401 tatacttaaa aacgttttgt tcaaggatgt tttaggtaag tattctaact tttcattctc
    11461 taaatttaag atagtttcta tcttagattc aaattttgaa aattctgtat ttcaagaagt
    11521 aaaaggtgac aatctagtat ttgagtatac aaatttaaaa aaagcaaact ttaatggaac
    11581 taaactttct ggcatagatt ttactactaa taatattgag ggaattgaga ttggtataga
    11641 tgatattaga ggagcaattt ttgatgtgtc acaagcaatt gatttaacga aattgatggg
    11701 aattatcata aaatgaaaaa aatattcata aaataaattg ggaaaacgtt aacaatgtgt
    11761 gaatttttac cagattgaca tttttctgaa aataaagtaa gattatctta atattcaaaa
    11821 tattttcaaa gggggtaatt tagtgtgagt aagcagatga gagatatttt tatagttggt
    11881 tttgctttat tttctatgtt ctttggtgca ggaaatctta tgtttcctcc atttttaggg
    11941 atggaatccg gcaaagactg gttaattcct cttattggat ttgttgttgc agatgctggt
    12001 atagcaatgc ttgttattat agcaacagca agatgtaagg gaagcatgga tgatgtactt
    12061 aggagagctg gtaaagggtt agcaagagta atgagtgtag ctgctctagc atgtttagca
    12121 cttcttgtaa taccaagaac ttgcgcaaca acatatgaaa tgggtattat gcctatattt
    12181 ggagaccatg gtccagtagc taaagctgta ttttcaataa tcttctttgg gctaacttta
    12241 gcatttacta ttaggtcttc taaggttata gacataatag gtaagtattt aactccagcg
    12301 ttattattag tattatttgt tttaatagta aagggtataa tttctccagc aggacctatg
    12361 agtcctgagc atatgataga taaaaatcta tttggtgaag gtatatcaca aggttatcaa
    12421 acaatggacg ctctaggtgc agcatctatg gcaactataa tgttgatgtc tataatagct
    12481 aaaggatata catcagataa agaccaaata ggaatgacta ttaaagctgg ttttgtagcc
    12541 tgtgtatttc tagcagtagt atatggtggt cttacttatt taggtgcaac agtttcaact
    12601 ttatatgata ctagtatacc tcaagcatct ttattagttg aaataactag tatattgtta
    12661 tatggtcaag taggaaaggt aattttaggt gtaattgtgg cacttgcctg tctgactact
    12721 tctacaggtt taacagcatc aatatcaact tattttgaag gaataagtaa ggcgagttat
    12781 aaacaactgg ttataggtat atgtgtagct agtatgctta tatcaaattt aggtgttgat
    12841 tctataatag ccatatcagt tccaatccta caaactatat atcctgtttt attagcaata
    12901 gttgttatgg agttggctgg taaacatata aagaatgata atgcttttaa aggtgcagca
    12961 tatgttacat tagtgattag tttattatca gctataaatg gaatgacagg agctgttcca
    13021 tttatacaaa gcttgccact tgcaggtttg ggctttaact gggtaatacc agctatattg
    13081 ggtggaatag ttggtaactt tataaaaagc aacaaacaag tagcataata atttgtaatt
    13141 aatttaattt tataatatat taatttttag tactatgggt aggtttaatc taaacctaat
    13201 ctacccatag tactttttag attggattat aatagcatgt atataatttt ttataatata
    13261 atttttcttt gaagtatgat aaaattagta atagtgataa gaatgtacaa atttaggagg
    13321 ttttaattaa tgcttagaaa aataacagca tttatgctat ccatatgttt aattattgga
    13381 cttacaggtt gtagtttact tgtaggcaag aaagataaag agaaaattat agatgatttt
    13441 aaagttgcag tggtaactca accattatct gaaaataaag tacaatacaa tatggtggaa
    13501 gagatggcaa aagaatatga ggaagagaat aaaattgata aagacaaaga tggtcaaact
    13561 aaagttaaac aaactataaa gcatgttgta ttgccagaga attttacttc taatatagac
    13621 agtgctatta ataagatagt aaagttggct gacgataaag aagttcaggc tattgtagtt
    13681 tcaactgacc aagcagggct tttacctgct ttacaaaagg taaaggaaaa gcgtccagaa
    13741 ataataacta tttctgcccc tatgggagat gataaaaacc aattaagcca atttgttgat
    13801 gtgaatctag gggttagtgc agaggaaaga ggaaaagtat tagctgaacg ttcaaaagaa
    13861 atgggagcaa aagcttttat tcattatgcc tctacagatg atttgaaaga tgttaatata
    13921 gccaaaagat tagaaatgat taaagagact tgcaaaaata taggattgcc atttgtacaa
    13981 gttaatactc caaatataaa tactgaagaa gacaaaaaca aagttaagca gtttttaaat
    14041 gaagatattg aaaaacaagt taaaaaatat ggaaaagata taaatgtatt tggtgtaaat
    14101 gagtatatgg atgaagttat attgactaag gctttagaat taaagtatat agttgcagag
    14161 caaagcaatc catcaccaat acaaacatat cctagtgtta tgggtctaaa aatatctgaa
    14221 aaagatgctc aaaattatga taaaataaac gacatgataa gtgaaaaggc taaggctttt
    14281 ggcatgtcaa atagacttgg tggatatcct atgccaatgg atgcttttct accatcagta
    14341 gcaatttatc ttgctactga aatggtgaaa caagatttaa cacaagaaga tgtatgtgac
    14401 ccagattatt tagaggcgtt tacagaatta agatttggaa tcggttcaga atttactcct
    14461 ttaacagagg tactttataa ttatcaatca gtaattttaa gccaattaat ttattaatat
    14521 taaggaagtg tctcaaaatg aactttttag tttatgaggc actctttttc tattaaatca
    14581 aatatatttt tatcttttta acaattaaaa agaagcttat ccctatattg agatagcttc
    14641 gtttttaata taaataagtt ttattatttt ataaaaaatt tatagaaaaa taaaaaatta
    14701 taattatata taaattttga taaagtaaag atgaattact ataggtttaa attatattat
    14761 tttctattat ttttatgcct ttctcagtta ttgatgagcc ttttcttcct ttactagaaa
    14821 ttaagtatcc attgcgtgtc aaatattcta gcatatttct tacttcgtat tctgtgacat
    14881 tatattccat aaatttcatt aactttgtcc tccccattgg tttcatgtgc aatgatttta
    14941 ggatagaaat gcatatgttt ttgtcatatt ggtttaaatc attgaatatg gacatattac
    15001 ttgacatatg attattattt aattttgaag atatgttttg aggtaaatca tttattgtaa
    15061 gataattgga acccatgtaa gccatatatt caatacaatt tctcagttct ctaatattcc
    15121 ccatccaagt gtatgaattt aaagcttcta aaagactctt atcgagtttt atgtttttat
    15181 agggaagttc ttttaaaaat gattctaaaa gaagaggtat atcttctaat ctctttctaa
    15241 gaggaggtaa atctaaagtg aacatactta tcctataaaa taaatccatc ctaaatttat
    15301 tttctataat taatttctct aaatctttat ttgtggcagc taatattctc acatcaattg
    15361 gaataatact tgtaccacca atttttctaa tttctttttc ttgtaagact cttaaaagtt
    15421 taacctgtaa atcatgtggc atatctccta tctcatctaa aaatatagtg ccagtgtgag
    15481 ctaattcgaa taatcctttt tttccacctt tttttgctcc agtaaaagaa ccatcttcat
    15541 atccaaatag ttcactttca agtaattcac ttggtaatga agcacaattt attgctacaa
    15601 aaggtttatt ttttctattt gaatgattgt gtattgactg agtaaaggct tctttacctg
    15661 ttcctgtttc acctgttatt aatataggat tatctgtaag agccattttt tttgcctttt
    15721 taatacaatc ttttataata gaactatttc caataatatt attaaattta tatttagcta
    15781 cataacctct tttgacagaa tcacttctta ttttttgttc taatatttct atattgttta
    15841 catctttcaa tgtaattaag caaccctcta tatttttata aagataaaat ggttttttag
    15901 aaagtatgaa ttttttatct atttcgtcta tgtatataat catattatcg atatttaagt
    15961 tctttgtaat tttatctaag aaactatttg gcatatattc cattaaggaa ggtgatttta
    16021 taagtttttc atctagtccc aacatattaa gtaaagactt atttacaaaa gtaaccttgt
    16081 taaaggtgtt gaaaaatatt agaccatcac ctatttcatc aatggtcatg taaagtatag
    16141 tttttaattt agatatatta tctaatgatg tatttaagaa atcatgactt agtgtctctt
    16201 tggacaattt gtatagtttt tctataaatt tctcattttt tagttttaat actactaata
    16261 atgacatata tgtatctaat gagatttttc tccaacctaa atcaacaata ttttttatgt
    16321 attttggtat aaatctaatc tgacctggtg ttatagctgt atctaaatca tatatttcat
    16381 cacaatctgg atatgtcggt acaaaatcta tatgatttat accaatttta tatagataag
    16441 tagccatctc aaatgctgaa gttttattgg ttgtcataag taacgatttt gtacctggag
    16501 gtagtttcat cattttttca tatccaagtt ttgaaagtgc tttcgttcca tgaataattt
    16561 caatatcttc cttaaaatgt tttattaaaa aattactagt taaaggtgat agagaaagca
    16621 ctacatctcc ctctaaattt tcttttattc catcaagtat attttgtgtt tctatgatta
    16681 tttcgtctcc aaaaaagttt gttaattctt ctttgtagtg gtcactattc aatttatcat
    16741 gtgttataag gattaacttc tttttatcca ttttatttct cctaacaaaa tagtatttat
    16801 ttttaaagta tttaaagtat aaggcaagtt atattataca acaaatagta gtataaattt
    16861 atatatttga ttaaataata ggttttttta ggattttatc ctataaaaac ctattttaaa
    16921 attatttttg caagataagg aaaatagctt tttatattgt aatgtatatt ttaaataatt
    16981 aataaaaaaa tattatattt ttaaacttga aaagtaagta attataagct atttaatcac
    17041 tggaataaat ttacaataaa aaaataaata caaaggcttt atcattaagg tattggcata
    17101 agttttgctt attaaacaag tatactaata aaattcattt aaacaggagg gagaatttat
    17161 atgagtaaat tagaatcaac aacagaaaaa gttgaggtaa aatggtatgg gtatgtagca
    17221 ttaatattgg gagcattatt tttttcagga atatttaaag atgcaccagg accattaaaa
    17281 gtattggatt ttaacaatgt attaggttct tttggaacat tgggaacaat taatgatggt
    17341 gtaggtacat tagctgcaaa ctttagaggt gatggaggta ctggccctcg agatggatgg
    17401 ctatatgcat tgacattaat accatcagtt atgtttgcac ttggagtagt tagagttata
    17461 gaccacttag atggaatgaa ggcagctcaa aagcttctta gtccactatt aaagccatta
    17521 ttagggttac ctggatttgc aggactaact ttgatagcta gtttacagtc tacagatgca
    17581 gctgcatcta tgactaaaga acttaaagat gatgggtata tagatgaaaa acaaaaagct
    17641 gtattttgtg catttcaatt ttcaggtgcg tctgcaatta cgaatttctt tgcttctgga
    17701 gcagcactat ttccatttat aggagatgta ccaatattta taccacttgc tttaatatta
    17761 attatgaagt ttgttggggc gaacttactt agattatatt taaataaatt tgacaagggg
    17821 gaagcttaac atgagtgagg tagcaaaaaa agaagtaaaa gttaaaaaag gaaatgtgat
    17881 agatgcgttt attggaggag ctagaaatgg attccaaata agtacaaatt caatggctcc
    17941 aaatgtcatc ttcggttttg ctataatttc tgtgttcaat ttaacaggtc ttttggatgt
    18001 aatagggaca atatttacac ctgtaatgag tatctttgga cttccaggtg ttgcagcaac
    18061 agctataatg acaatattta tatcaatggg aggagcatct ggagtaatcg caggtttatt
    18121 tacagcaggt caaataagtc aagcacatgt tgcaattcta ttgccaatgt tattgctttc
    18181 aggagcgcag atacaattta tggggagact tttaggaaca gctgagttaa aaactaaata
    18241 ttatccacat atgtttgtta tagcaacttt aaatggttgt attgcaatgc ttataatgaa
    18301 ttatttcgta gtataggtaa ttataataaa gatttaatat aaatggaaag tttaaatttt
    18361 aaggaggagc tagattatgg aaactaacaa agactatatt gagtatgtac ataaagtgta
    18421 tgattatcta cacactatac cagaaaaagg atttgaagaa tataagacat cagagtttgt
    18481 tttaaaagag cttgaaaaaa tggggtttga aaatattaat aaaggaattg caggaactgg
    18541 tattatagta actatagatt ctaaaaaaga aggaccagta tttgctataa gagcagatat
    18601 ggatgcattg gaatttatca tagatggaga aaagaaaatg atacatgctt gtggtcatga
    18661 tggtcatact tctatgcttt taacagctgc aaagttaata aaagagagaa atttagtaaa
    18721 aagaggtaag ttaaaaatac tttttcaacc aggtgaagaa aaattatatg gagcattaag
    18781 agtaattgat tctggtttgt tggatgatgt agaagaaatg gtagggatgc atttaagacc
    18841 tggacaagat tgtaggttgg gaaaggcgat acctgcaatg taccattgtg ctagctgtat
    18901 aatggaattt acgattaaag gtatcacttg tcatggttct aaaccacatt taggagtaaa
    18961 tgcagtagat gctgtctgtt tagcagtgaa tgcaataaat gcaataagag taaatccagc
    19021 tataccaaat tctataaaag taacaaatat atcagttaag ggagaaactt ataataatat
    19081 accagatgaa gcatttatgg ctttagatat aagggcagaa agtaatgata taatagaaga
    19141 attaaaagaa aaagcaatac atgctgtaaa aactgctgta agttctataa atgcagaagc
    19201 tgtactaatt tccaaagatg gagtaccagc agcagaattt gatgaagaac ttgtaaactt
    19261 ggctgaagaa actataaaag atgtattggg agctgatgca tctataggta tatttaaaaa
    19321 ttctggtgga gaagatttcc attattatac acaaaaacta aaatgtaaaa ctacatatat
    19381 tggattagga gctgatgcta caccaggatt tcataatcca aatgtaacat ttgatacgaa
    19441 agctttagaa tatggtgtag atatatggtg tagattggtt gaaaagagat tgggataaca
    19501 tccaaactaa taattctaca tttaaaatta taattataga agttatagaa aaagattgct
    19561 tatgttaatt agaaaataat taatatgagc aatcttattt tgatataatg aaactgattt
    19621 tggaatattg aaaaaatatg atggaaaagt aattatattt taaatctatt ggacaaaaaa
    19681 gtgatattat tataatatta agaattgtaa aataatacca aagtgcatct agtaatagac
    19741 ttaacaatta taaaaatttt attcttagga gggaaaaaat gagtaagtat aattttgaca
    19801 gagtaataga tagagtagga acagattgtg taaaatggga ttttagaact aattgttcaa
    19861 caaaagcaca aaaagatggt ttgccttttt ggattgctga tatggatttt gaatgtgcag
    19921 aaccaataat agaagcatta cgtaaaaaag ttgaacataa aatatttgga tatagttcaa
    19981 atgatagtga taaatacttt aatgcagttt gtgattggta taaaaggaga tttaactggg
    20041 aaataaatag aaaagatatt atttttagtc ctggtgttgt acctgctgtt gctattttag
    20101 taagaatatt aactaattct aatgaaggtg taatagtcca aaaaccagtg tattacccat
    20161 ttgaagctaa gataaagagc aataataggg atgttgtaaa caatcctctg atatatgaga
    20221 atggtactta taggatggat tatgatgatt tagaagaaaa agctaagtgt agcaacaata
    20281 aagtactgat actttgtagt cctcacaacc ctgttggaag agtttggaga gaagatgaat
    20341 taaaaaaggt tgtagaaata tgtaaaaaat atgacctatg gataatagct gatgagatac
    20401 attctgattt aattagaaaa ggatttaaac atactccttt acagtcatta tgcccagagt
    20461 acaaggataa aatagttaca tgtatagctc caagtaaaac atttaatctg gcaggaatgc
    20521 agttgtctaa tataatagtg aataatgatg aactaaaaaa gaaatatcaa gaagaggtta
    20581 cagctgttgg agttgcaact tcaccaaatc cctttgctat tgtagctaca atagcagcat
    20641 ataatgaaag tgaagactgg cttaatgaat taaatgatta cttagataat aatatacaat
    20701 ttattgatga atacttaaaa gaaaacctac caaaagtaaa acttgtttat ccagaaggaa
    20761 catatctagc atggttagat tttactgctt atggattaaa tgaagtagaa cttgaagatt
    20821 taatgtttaa aaaagctaat gtattatttg atgaaggtta tatatttgga aaagaaggaa
    20881 taggatttga aagaataaat gtagcttgtc cacagtcttt attaaaagaa tgtatggaca
    20941 gattaaagac tatatttgaa aatttataaa gtataggtaa aaatttataa agtgtttaaa
    21001 aataaaaaaa taataaactt gacaaatttt aactaatcac ttatcatact attatgaata
    21061 aatagaaaac ataagagagc taaattttat tgaattaaac tcttaaagac tttgaaagga
    21121 agagtagtta tagaatgtaa ttaaagcgag ttggtgatgg tgtgagacca atatgaagtc
    21181 tataatgaag aacatcctgg agttgctaac cgaaatttta aaagagtagg cttagtcgga
    21241 accaaaccgt tatcatattg gggtgtatcg agtaatatct gtacattgtg agctgggttt
    21301 gaatttattt caaactaatc agggtggtac cgcggaaaag caacctttcg tcccttttat
    21361 atagggatga agggtttttt taatggaaaa atatataaat atagggagtg aatgatatga
    21421 gcacaataat accaaaggat tataaaagta gtttgaacgt aattgataca caaatagcta
    21481 taaaaaaatt aaaagatttt tttgaaatga gactaagtaa tgaattaaat cttataagag
    21541 tttcttctcc gttatttgta ttgccagaaa caggtgcaaa tgataattta aatggagaaa
    21601 aggctgtaag ctttgatata ccatttatga ataaaaatgc agaaattgtt caatcacttg
    21661 caaaatggaa aagattagcc cttaaaaaat atgggtttga agttggtagt ggtttatata
    21721 cagatatgaa tgcaataaga aaaaatgaag aattagacaa tatacattct ctttatgtag
    21781 accaatggga ttgggaattg gttatagata aaagtagtag aaatgaaaag actctaaaag
    21841 atgttgtaaa aagattgtat ggagtattta aagatactga aatttttgta tgtagtatgt
    21901 atgaaggtat aaaagaaata ttaccagaag aaataacttt tataacatca caagagttag
    21961 aagatatgta tccagaatta tcaccaaagg aaagagaaga taaaattgta aaagagaaga
    22021 aagcagtatt tataactcaa ataggaaaaa cattaatatc aggagaaaaa catgatggaa
    22081 gaagccctga ttatgatgat tgggaattga atggggatat attattttac aatcctgttt
    22141 tagattcagc attagaatta tcttcaatgg gaattagagt tgatgaagaa tctcttgata
    22201 gacaattgaa acttgctgga tgtgaagaga gaaaagagtt tgactatcat aagatgcttt
    22261 taaatggcga acttccgtat acaattggtg gaggaattgg tcagtcaaga atatgtatgt
    22321 atttcttaca aaaagctcat ataggagaag tacaagttgg agtatggcca caggatatga
    22381 ttcaaaattg tagtagtgca ggaattgaat tattataatt taaatataaa ctaattgata
    22441 aaatttatat ctaatatgtt catcaaatta attatatcgg ttataatgta gaataaccta
    22501 taattataag aggtgataaa tatggagttt ttaaaaataa tcattaatat agttttagat
    22561 attttaaaaa aaattttggt cagatttaaa aatgctaaat ttggtttggt ttttgtattt
    22621 gatttactta aactacctga ttttatgaca gataaaagaa tcaatatagt tgataaaata
    22681 aaggtaattt ctgtattaat ttttactata tcctactttg tatctggggt tgatataatt
    22741 ccagagatga ttgcaggggc atttggtttt atagatgatg ctatagtttt aatttggagt
    22801 ataggtatag tgaatgaaga aattaataag tatagagtta tcgcaaaaaa agataaacat
    22861 tcaaatataa ttgaaaatgt agaattttct ataaaagatg aagaagaata acaatttata
    22921 tatgttattc ttttttttat taacaaaaaa tggttaaatt gttgacggag aggtaaaact
    22981 ctattatcat aataagtgga tatccaatat ttaactaagt attcaatgtg caagaatgct
    23041 taatttaaaa aagttatgga gggtaaatat tatgaagaaa aaaataggtt tagtgcccaa
    23101 actaatcatc ggtatcatag tcggtatttt aatcggaagt tatgcaccag aaataatagt
    23161 acaaatatta gtaacagtta gtactttatt ctcagcattc ctaaaatttg ttataccatt
    23221 tataattata ggttttgtaa cagcaggtat agctgattta gcaacaggtg caggaaagtt
    23281 attggggata actacgggaa tagcatatgg ttctacctta atagcaggat tattatcatt
    23341 tgcagtatct acattaatct tcccaaattt tattgatgca agtgttgcaa gtcaaattgg
    23401 agacccagag gcaggaatgt tagctccaat attcacaatt ccattagagc ctatggtaga
    23461 tgtaacagct gcaatcgtat ttgcatttgt aatgggactt ggaatatcag ctttaagaaa
    23521 tcatggaaaa ggagagactt tatttaattt cttccaagaa tttcaaggaa tcgtaacgaa
    23581 aacgttgtca acagtgatta tacctttatt acctttatac atagcaggaa cttttgcaaa
    23641 tataactata gcaggggaag tatggacaat attaggggta ttctggaaag tatacttagt
    23701 agtaatacct ttacattttg tatatatggc atgtcaattc acagcagcag gtgtattttc
    23761 aggaaaaaat ccagtaagaa tgttaaaaaa tcaagtacct ggatatttaa cagctatagg
    23821 aactcaatct tcagcagcaa ctataccagt taatgttggt tgtgcagaaa aaaatggagt
    23881 aagtaagcaa ataagagaat ttgttgtacc tctttgtgca acaatacatt tatcaggtag
    23941 tatgatatca ataacaggat ttacaactgc agttttaatg atgaatggta tgccacatgg
    24001 aataggtgta ttattccctt atattgcaat gttaggtata gcaatggttg cagcaccagg
    24061 agcaccaggt ggggctataa tgagtgcact tcctttccta ccaatggttg gaataccatc
    24121 agatggtggg cttgcaagtt taatgatagc tttatactta actcaagaca gttttggtac
    24181 agcatgtaat gtatcaggag ataatgcaat agcagtagta gtagataaaa taaatgatgg
    24241 aatgagtaaa aaaggaaata aagaagagca aagtgcttag ttttttaata atcaaaaatg
    24301 agttatctca aatagagata gctcattttt tatggtataa aaaattataa aataaagtga
    24361 tttttttatt taaaaacaca ataaaaatct gattatcccc taaaacacag tatctatcag
    24421 cattttcaag gaaacaaaca catcaattta ctacttattt actactaacg aatgagcctt
    24481 gatacgcaag ttttcctaga tacgcaaaga tatggtacag cacatcagta ttgaaacaag
    24541 aaaaaattga ataacttata gactatggaa cgcctaacat gacgttcctt ttctattttc
    24601 agccgttctt attggacggc tattttatac ccaaaatgaa aggagcatta gatttatgaa
    24661 aaagatgtta aaacgattgt gtacgggctt cttagctctt gcaactgtcg ttactgcttt
    24721 accgactaca cccgttcatg cagaaagtaa gcaatattgg acggagtcaa aagagcgtgt
    24781 cggtatcgtt gaaaaagtaa tgaatgacgg ttcgattggt tctaccttta atgagggaca
    24841 tttgaccgtt gagggcgagg acgcttactg tatcgacatt aacacagatt ttaagaatgg
    24901 ttacaaaatc agaactgacg caagcacccg tatgagtgcc gaccagataa gcgacgttgc
    24961 cttatctatc gaatatgtga aacagtacac agacagccac agcggaataa gcaacaatca
    25021 cgcctacctt ttaagacagc ttgtagtctg gcagagattg agcgtacatc ttggctggca
    25081 atgcgacaac gtgagagctt cttatgatga aattccaaag gcaacgcagg acgaagtttt
    25141 cgctggtgca agagcctttg tcaaagaaaa taaaggacgc tatgaatgtg gcggttatat
    25201 ctactctggc gaggggcagg aattgggaca attctgggcg aaactgaatg tcggaaatgc
    25261 taaacttcaa aagacttcca ataataccag cattacagac ggtaacggga attactctat
    25321 tgcaggtgcg acatacggtg tcttttctga taaggactgc acgaaacaac ttgccaccct
    25381 tacgactgat gaaaacggaa atacagatgt tgtagaggta aaagcaggca cagtctatat
    25441 caaggaatta tccgcaccag caggatataa agtggataaa acagtctatc cattaacaat
    25501 caaagctggc gaaacagcga ccttgaacgt atctgatact ccaaaagtaa cggacacttt
    25561 gattgaactt ttcaaaattg atatggaaac acagaaagac aatccgcagg gaaacgcttc
    25621 tttagcaggt gcggaattta catggaagta ttatgcaggt ttctacaata aagacaatct
    25681 ccctgccgaa gctactcgta catgggttac aaagacagtc gccgaaacag acagcgacgg
    25741 ggcaacccac tacatcacaa aattagcgga cgcatacaag gtatctggcg acagcttcta
    25801 tatgcaggga gttaaggcgg ttcttccact tggtacatta actgttgagg aaacgaaagc
    25861 tccaaacggc tacttgctgg acggtgcata tatgcaggct ggggataagt ccgaacagat
    25921 aaagggatta tatgtaacac agattaccga ggacggcgac cttgccgtat tgagtggaag
    25981 taaccagttt tccgtatcgg acaaggttat ccgtggcggt gtgaaaattc agaaacgaga
    26041 tttagaaacg ggcgatacga aaccacaagg aagtgcaaca ttgaaagata ctgcatttga
    26101 catcatttct ttaaatgata atgcggtatt ggttgagggc aagttataca agaaaaacga
    26161 agtggtaaag acaattcgta cagatattga gggtgtcgct tctacttctg ccgacctctt
    26221 accttatgga aaattccgta tcattgagag cgaagctcca gacggatatt tgacagacgg
    26281 tgcgaaaccg attgactttg caatcacaga aaatggtaaa attgtggact taaccgacga
    26341 agcccactct atctacaatc agattaagcg tggagatatt gagggtgtga aaatcggtgc
    26401 aggcacacac aagcgtcttg ctgatgttcc ctttaggatc acaagcaaga cgacaggtga
    26461 aagtcatgtt gttgtaactg atgataacgg gcaattctcc acttctgctg actgggcttc
    26521 tcataagcac aataccaacg caggaaaaac cagcgaggac ggtgtgtggt ttggaacttc
    26581 tgaaccagac gacagcaagg gtgcgttacc ttatgatact tacatcattg aggaattacg
    26641 ctctgacagc aacaaaggct ttgaacttat cccacctttt gaaatcgtgg tatcaagaaa
    26701 taaccttgtg attgatttag ggacgctgac tgatgaatac gaaaaagaaa tctctatcca
    26761 taccacagca accagcaaag acggcgaaaa gactatcctt gcaggaaaag aggttacgat
    26821 tgttgatact gtgaagctgg acggcttgga aaaaggtaca aaatatcagc tcaaaggctg
    26881 gcagatgtta aaggaagaaa acgctgaact tatcattgac gggaaacgtg tagaaaacga
    26941 ttataccttt gtcgctgatg aggaagaaat gaaagtagaa atttcctata cattcaatgc
    27001 gtctgcttta ggtggcaaga accttgttac ctttgaagaa ttgtatgatt taagcaatcc
    27061 agacgaaccc gtaaaagttg cggaacacaa agacattgag gacgacgggc aaacggtact
    27121 tatcacagag cgtatcatca aaattcatac gactgctacc gataaggacg gcaacaaaga
    27181 gcttgaagct ggaaaagacg ttacaatcat tgataccgta accttagagg gattagaagt
    27241 cggtacacag tacaaacttg tgggctggca gatgttgaaa gaagaaaata tagagcttct
    27301 tatcaatgga aaacgtgtgg aaagtgatta tacctttacc gcagatagcg aaactatgaa
    27361 agtggaagtt gtctttacct ttgacgctac ttctcttgac ggcaaacagc ttgtaacttt
    27421 tgaggaattg tacgatttaa gcaatccaga cgaaccgaag aaagttaccg agcataagga
    27481 tattgaggat aagggacaga cgattacctt taaggaaaag ccagaagaac cagaaaaacc
    27541 cgaaacacca ccgacaccag aaaagcctag cagaccgagc gacagtccca aaacgggcga
    27601 cagtacaaat gtaatggcat ttgttgtgat gttgcttgcg tctgctggtg gactggcagg
    27661 tatcttcttc tacaaacgcc gtaaaatgaa gaactcataa ggcggtaacg gtatgaggaa
    27721 agaaaaatca cgctctccgc cgaagcggtc aaacggcaga cttccgctta ttctggcttg
    27781 tctgccacca acctgcaaga acacggatag tcaagggtgc gtaagcattg attttaccct
    27841 ttactatctg ggggattgct ggtacttccc gaaccgccag aataagaaat cacaatcaat
    27901 ttatcaaaaa tagaaagaaa cgaggtaaaa caatatggaa atgaaatatg tcgtgccaga
    27961 tatggcacag tcttttggaa ctcttgaatt tgcaggcgaa agtgagccag tctttgaaag
    28021 agataaaaat aaccgcaggg tcttagccag acgaagctat aacctttatt ctgacgtaca
    28081 gaaaggcgaa aatgttgtgg tagaaattcc cgtgcaggct ggcgaaaaga agttcaagta
    28141 tgagcagaaa gtaaaacttg tcaatccgaa gttatacggc agaggttacg caatcgggga
    28201 tatgggacat accgattatg tgttagttgc tgatgatatt gtagcagttg aagaaaagta
    28261 aaggagtgaa gaatttatga gattatcaaa cggttttgtt attgacagag agaaaacatt
    28321 tggagaatta aaatttacag cggtgcgtga tgtgttcttg cagaatgagg acgggacacc
    28381 gagtacccag cttaaaaagc gtatctatga tttgaagtgc agtctgcatg gtggaattat
    28441 ccccgtgtcc gtaccgccag aagtaccgtt aagggaattt ccgtacaatg cagtcgtgga
    28501 gcttgtcaat ccagtagccg atacggtatc acgaaaaacc tatacgggtg cagatgtgga
    28561 ctggtatgta aaggcagagg atattgtatt gaagaataaa ggcaaccaga acgcaggcag
    28621 tccacagaac aacaattcgc agggacagca gaaaaaatag gcttgctgaa tagattttca
    28681 ttgtagcgga tatgtgatac ctgctataat aagtggtata tcaaaatgta ggagtgaaac
    28741 aaaatgaaat atatttttct acatggactg gggcagactt ctttcagttg gaataaaaca
    28801 ttagaagtaa tgagtgataa gtgggatata ttgtgtccta atcttgcaga ctggctatat
    28861 aatgaacgcc cctgctatga aactttatat aaagcgttgg aaaggtgctg taaacagttc
    28921 aacgaaccgc taacgctgtg tgggttatct ctaggtggta ttcttgctat gcaatacagt
    28981 attgaacatt cagaaaaggt acattcttta gtgctgatag gaacacagta tgcaatgcca
    29041 aaaaaattac taaaaatgca aaatatcatg tttcaattta tgcctagctc aatgttttca
    29101 aaaatggggt ttcaaaaagc agattttatc agcttatgca agtctatgat tgatttagat
    29161 tttacccata atttgaaaga tatttcttgt cgtgtattgg ttatatgtgg ggaaaaagat
    29221 aaaccgaata aatcagcgtc cctacaatta ggaaatttaa taccatacgc aaaaattacg
    29281 attatatctg gtgcaggaca tgaagtaaat atagacgctc ctattaaatt gggaaatgaa
    29341 ttaaatgttt ttttcaatag ttctcataat accgaaaggt aaaaagcaaa gtaatctaaa
    29401 agctgtaaat gcctataaat acgctgttgc aatggttcgt tgcgttaatt ccagctaatg
    29461 ttggtagaca taggacattt tattttttat gattttatgc aaataacgat aaaaggagaa
    29521 tgtctatgac acttggagag aaaatacaaa aattaagaaa acaaaatggg ttttcacaag
    29581 aagaacttgc agagaaagta acggttactc gacaaactat ttcaaaatgg gagctaaatc
    29641 aatcagaacc agatttagac tttatagctc aacttagtaa cattttcaat gtatctgccg
    29701 attatcttat caaggaagaa ttaacgaagc ctgatgaatt accattcaga aaaaagaggt
    29761 atcaatatta cttctctgaa agaagcaaac gcacaatgtt tgttgcaata tcaatagttg
    29821 ctttaatagc aagcatagtt tgtcttattt gtgattattt tacttctgat aagctatctt
    29881 ggtcttttat agctattgaa gcaattattg cagtttggct tgtattcctg ccttgtatgc
    29941 ttttcaagga aaaagtaatt tttaagacat tgataatatg cagtattatt cctatcccat
    30001 ttttagcaat acttgccttg ttattaaagg taacgaccat ttttacactt ggcagttgta
    30061 tttcagtcct ttgtattgcg attatgtggg ctatttatgg aatttttcgc aagtatcatc
    30121 aacgcatatt tcttagttta gggttttctc tgttactttt gatatttgtt ccgattgtca
    30181 ttgttcgtgt tacagactat ttcttaccgc aatatgaaat tgtttcaaaa tctgatgtat
    30241 ttaatggcat aatcacattt gctattgccc ttatatgttt tggtatagat tacttaacac
    30301 aacataaaaa taagtagata ttatttcaaa gcagttagtc ttaggattga ctgctttttt
    30361 cgtacccaga aaggagtgat tgatttatgc gtaagatttt taacaaagga caccgtatca
    30421 gagccagcga caagcacctt gtctaccact actgcatagg gacgcttctg tttgtattca
    30481 tggcggttct cctgctattg aatagcaaac agctcatgcg taccaactgg gagcatttca
    30541 gcttgttaca aaatggcttg atactttccc cttacaattt cataacgata ctgatagcga
    30601 ctggtgtttg tgtattggtc gcttttctgt attaccgctt ctgttacgac agtttcaaga
    30661 agctcctgca ccgtcaaaag ctggcacgaa tgatactgga aaataagtgg tatgaagccg
    30721 ataccgtaca agacagcggt ttttttactg acctgcaaag cagatcaaga gaaaaaatcg
    30781 tctggtttcc aaagatttat tatcaaatgg aaaagggact gcttcatatc tgctgtgaaa
    30841 ttacgctggg aaaatatcaa gaccagcttt tacggttaga ggataaattt gaaagtggct
    30901 tgtattgtga gttgaccgac aagaccttgc atgacggtta tatcgaatat accctgcttt
    30961 atgatatgat agcgaaccgc attaccattg atgaagtacg ggcagagaat ggctgtctta
    31021 gactaatgaa aaatcttgtc tgggaatatg acgcactccc tcacgccctt atcgctggtg
    31081 ggactggtgg cggtaaaacc tattttctgc tgacgctcat tgaagcctta ctgcacacaa
    31141 acgctgtcct ttatatctta gacccgaaga atagcgacct tgccgacttg ggaacggtta
    31201 tgggaaatgt gtatcatacc aaagaagaaa tgattgattg cgttaatgct ttttatgagg
    31261 gcatggtaca gcgaagtgag gaaatgaagc gacacccgaa ctataagacg ggcgaaaact
    31321 acgcctatct gggactaccc ccctgctttc ttatctttga tgaatatgtg gcgttttttg
    31381 aaatgcttgg gacgaaagaa agcgtgggtt tacttagcca gttaaagaaa atcgttatgt
    31441 tagggcgaca agcaggttat ttccttatcg ttgcctgcca gcgtccagac gcaaagtatt
    31501 tctcggacgg tatcagagat aacttcaatt tccgtgtggg acttgggcgt atcagcgaat
    31561 taggttacgg tatgctgttt ggttcagatg tgaaaaagca gtttttccag aagcgtatca
    31621 aggggcgtgg ctattgtgat gtgggaacaa gcgttataag cgaattttac acgcctttag
    31681 tcccaaaagg acatgatttt ttacaaacaa tcggcaggct tgcacaagaa agacaagtta
    31741 tgccagaaca acaaggaaag gagaactaac tcatggaaat aaaagaaatc aataaaataa
    31801 tacaatcaga agataaagac gagaaagtta agagcgtaaa atgtatctgt atcaattatg
    31861 gagattttct agccgattgc gagggatttg tgcagaaacg ttatcacgat tttaaatgta
    31921 atccaaaaca tcagtttgag aaaaaagctg atactattct tgaaaatgca attcacgaaa
    31981 aaaacttcat gcccgatttg ttcttgatac gcttaaatag aaaacagtcg gcttgcaaca
    32041 gccagattga ttttgttttt gaattgctgg ataagtcttt tctggaaaca gaccctatcc
    32101 gtaaaagtga aatatcagaa gaaacattaa atttatgttt atccgctgat gtttcattca
    32161 tcatggttta cattggtatg aacaggtagg ataggttctg cctgcacaac ggcaggaacg
    32221 gtcggcggag caaatacgac cgtggaagcc gttttgcaga gcagggcttg tccctgctct
    32281 gctgatgaac ccctcggtat ctaacagagg ggtacgtttg caaatagaca atagacaaaa
    32341 aacataggaa acataaggct tctgacatgg tttcctagca aaaatcggct gtgaagtcgc
    32401 cttaaaaaac ttcacacgtt acagattggg ggtatggttc tgaatgaaga acaatggata
    32461 aaagaattac gggagaaacg gattgcttac ggtatctcac aaggcaggtt ggcgttagct
    32521 tctggcatta caagagaata cctcaacaag atagaaagcg gaaaaatgaa gccgtcaaag
    32581 gaacttctta atattctgca tgaggaactg gcaaggttca atccagaagc accgcttacc
    32641 atgctgtttg attatgtgaa aattcgtttt tccacgctgg atatacagca catcatcaaa
    32701 gatatattaa aactgaatat caattatatg ctccatgaag attacggaca ttacagttat
    32761 acggagcatt actctttagg ggacatcttt atctatacgt cgtctgacga agaaaaaggt
    32821 gtccttttag agttaaaggg tcggggttgc cgacagtttg aaagttacct gctggcacag
    32881 caaagaagct ggtatgactt cctcatggac gcactcatag acggtggcgt gatgaagcgt
    32941 atcgaccttg ctatcaacga ccatacgggc attttggata ttccagagct tgcggaaaaa
    33001 tgcaggaaac gggaatatat cgggaagtcc agaagttata agttctacca gtcgggcgag
    33061 cttatcaagc acagagagga cgacagagaa tatatggggc gtacccttta tcttggttcg
    33121 ctgaaatctg atgtatattt ctgtatctat gaaaaggatt atgagcagta cgtcaagctg
    33181 gggacacctc tggaagaagc cgacattatc aaccgtttcg agatacggct tcgcaatgaa
    33241 cgtgcctatt atgcagtacg agatttgctg acctattatg acgcagagca gaccgccttt
    33301 tctattatca atcagtatgt gcggtttgtt gatgaagaac cagacaagcg aaaaaatgac
    33361 tggaaactca atgaccgctg ggcttggttt atcggcgata acagacagag tttgaagctg
    33421 acgacaaagc cagagcctta caccttagac cgcacattgc gttgggtaca aaggcaggta
    33481 gcaccgacct tgaaaatgct gaaaaagatt gataaaggaa acggtacaga ctacatggat
    33541 acaatcgaac agcaggcaaa gctcacagaa aagcatgaaa tgataatcaa acagcagacg
    33601 acccctgcaa aagatttagt agaaagttag gagtgatgaa aaatgtgggt attattgaaa
    33661 gatttcctgc tggtatctat gggaatgggt attggcgtag tcttgatgtg tattttgaat
    33721 gttggcaaag aagctgacta taaaatgaaa caattagaag aaagcgagga caattaaatg
    33781 aatttcggac aaaatttata tcaatggttt ttatcaaacg cacagagctt agtgcttatg
    33841 gcgattgtgg ttatcggtat ctacttaggt ttcaagcgtg agttttccaa acttatcggc
    33901 ttcttggtag ttgccttgat tgctgtcggc ttggtattca atgctggcgg tgtgaaagat
    33961 gtactgttag agctgttcaa taagattatc ggtgcataaa attttattgt ggtgctgata
    34021 tgcgaatggt ataatgtgga tatgaaacac agtatctagg agtgatgaaa tgaaatatat
    34081 aatgcttcat ggtttgggac aatcttctga aagttggagt gataccgtaa aagtattcaa
    34141 tgatgatttt gaagttttat gccctaattt atcagattgg ctttctggga aaacaccatg
    34201 ttatgataca ttgtatcgga cattggaaac atactgtgaa cagtttgaag aaccgctaaa
    34261 tatttgtgga ctttctttag gtggtattct tgctatgcag tatgcgatag aacacccaga
    34321 aaaagtgaac tcattagtgt taataggaac acaatatgaa atgccaaagt atctgttaaa
    34381 aatgcaaaat ctgatatttc gtattatgcc ttgttcagcg tttgaaaaaa tgggctttcg
    34441 gaaaacagat tttatcaatc tatgcaaatc tatgatggaa cttaatttta aacatgaact
    34501 gaaaaaaata tcatgttcgg tacttgttgt ctgtggcgat aaggataaga taaataaaaa
    34561 agcttcttta gaattgcaac aacagataga aaacgcagaa atagctatta ttgaaaatgc
    34621 aggacatgaa gtaaatacag ataatccaac actattaggg caaaagttaa atgtattttt
    34681 caaacaatat gtctgataaa caatttcata tccctacaca caaagcagtt agtcttagga
    34741 ttgactgctt ttttcatact taaatttcag attggagtga ttacatgaac gatattttta
    34801 aggatatgca gtcaaaagtc ggctgtgatt atatttctga cctgccctct tacaagcgta
    34861 aggtatggca tgaaatgaaa cgactgaacc ttgctgacta tgaagaaaga cagttagaag
    34921 atttttctaa atatgtgttt ggtatgtcgt accagacctt aaaagatgtg atgaaacaac
    34981 agaaaggacg tgaggaacaa tgcaggaaac aagggtgctg gtggaaacga gaggaacaac
    35041 tggcgaagaa acaatatcat actggtttga actgccgata gatgttgccg agtttgaaga
    35101 aaagctaggt gtcggtgcag aaagtgggga ttaccgtatt attgaaaagg tgttgcctta
    35161 tgctgatgaa gtccacgaac atacaagcgt gtatcagctc aatgaattag attttatgta
    35221 ccgccagctt tcaagtgata tgcaggaaga atatgtatca ctacttacgg tgtttgaaaa
    35281 tttagaagca ctttatattt gcaggaacgt gattaccgtt tatccagact gcaaaagcat
    35341 gatagatgtt gcaaggcaaa agctgatgaa cgacccgaca tttaaacatc tgtccgagga
    35401 ctgtcaagaa tactatttcg attttgaagc ctacgcttct cacttacagg aacacgggaa
    35461 atttttagta acggaacacg gtatctttga actgccagag taggaaatga ggtggtgctt
    35521 atgattgatg atatggcggt ttatattgct aatcttggca aatacaatga gggctattta
    35581 gtcggtgctt ggttcacgtt tcccattgac gaggaagatg taaaagaaaa aatcggcttg
    35641 aatgaacagt atgaggaata cgctatccat gataccgaca acttccccat tgcgattggc
    35701 gagtatgttt ccattgaaga actcaacgag atgtatgaaa tgatagagga acttcccgac
    35761 tatatcgtag agtgtctgga cgaatttatc agccactttg ggacgctgga agaagtcgtg
    35821 gaacacaagg acgatattta ttattatccc gactgtgaaa ccatgacaga tgttgcctac
    35881 tactacatag acgaattgca ggcactaggg gatattccac ccagcttgca gaactacatt
    35941 gactatgaag cctacgggcg agatttggat atgggcggtt gcttcattga aacaagccga
    36001 ggtatgtgcg agataccata ctaacgctgg aagatcagcg acaagcattg ttgctactga
    36061 cagcgtattt ctctttaagg gacagtctga aaatggctgt ccttttccat ttagaaactt
    36121 acgaaaggag ctgaaagaac ttgaaaaaga ttaaatctta tacgggtatc tggaacgtgg
    36181 aaaaagtctt gtatgcaatc aatgacttta acttaccctt tcccgttacc tttacacaga
    36241 ttacatggtt tgtgattacg gaatttatca tcattctgtt tggggatatt cctccacttt
    36301 ccatgattga gggagcattt ctcaaatact ttggtattcc cgttgctctc acttggttta
    36361 tgtcgcaaaa gacctttgac ggaaagaagc cgtacagctt tttgaaatca cagataactt
    36421 atgcgttgcg acctaaaatc acttatgcag gaaaagccgt aaaactgcat aagcagacct
    36481 tgaatgaaac aatcacggca gtaaggagtg tgaactatgt tcccgataaa atatattgac
    36541 aataaccttg tctggaacaa ggacaatgag gtgttcgctt actatgagtt gataccgtat
    36601 aactattctt tcctatccgc agagcagaaa tttatcgtgc atgacagctt tcgccagctt
    36661 atcgcacagt cccgtgaggg taaaattcat gccttgcaga ttgccacaga aagctccata
    36721 cgaagtatgc aggaacagtc aaagaaactg gtaactggaa aattaaagga agttgcctat
    36781 cagaagatag acgaacagac cgaagcgtta gtatctatga ttggggacaa tcaagtggac
    36841 taccgttttt ttcttggctt taagctcatg gttacggaag aacagctcaa tctgaagaac
    36901 atcaaaaaat cggcgtggct gacgtttacg gaatttctcc atgaagtgaa ccacacgctg
    36961 atgaatgact ttgtttccat gccgaatgat gaaatcaacc gttacatgaa aatggaaaag
    37021 ttactggaaa ataaaatatc aaggcgtttt aaggtgcgtc gcttggaaat caatgatttt
    37081 gggtatctca tggaacatct ttacggcagg gacggtatcg cctatgaaga ttatgagtac
    37141 cagctaccaa agaaaaaatt gaacaaagaa acgctgataa aatactacga ccttatccgt
    37201 ccgacaaggt gtgtgattga ggaaagccag cggtatttac gattggaaca tgaggacaag
    37261 gaaagttatg tgtcctactt tactgtcaat gcgattgtcg gggagcttga ttttccgtcg
    37321 tctgaaatct tctatttcca gcaacagcaa ttcacattcc ccgttgatac ttctatgaat
    37381 gtagaaatcg tggagaaccg aaaagcatta acgacagtaa gaaacaagaa aaaggaattg
    37441 aaagaccttg acaatcacgc ttatcaagca ggaagtgaaa ccagctcaaa tgtggtggac
    37501 gccttagaca gcgtggacga gctggaaaca gacttagacc agagcaaaga aagtatgtat
    37561 aagttaagct acgtgatacg ggtgtctgca cccgatcttg acgagctgaa acgccgttgt
    37621 gatgaagtca aggactttta cgacgacctc aatgtaaagt tggtgcgtcc tgctggggat
    37681 atgctggggc ttcattctga atttcttcca gccagcaagc gatatatcaa tgactatgtg
    37741 cagtatgtaa aatcagattt cttggcaggg cttggttttg gtgctacaca gcagttagga
    37801 gaaactacgg gtatctatat gggctattcc gttgatacgg gaagaaatgt gtacctgcaa
    37861 ccgtctttag cttcgcaggg cgtaaaaggc acagttacaa atgcccttgc ttctgctttt
    37921 gtcggttcgc ttggcggtgg gaagtcgttc tgcaacaatc ttctggtgta ttatgctgtg
    37981 ctgtttggcg gtcaagcggt tatcttagac cctaaatcag agcgtggcaa ctggaaagaa
    38041 acgctcccag agatagcccg tgaaatcaat atcgtaaacc ttaccagcga caaggacaat
    38101 gcaggacttc ttgacccatt tgtgattatg aaagataaag aggacggagc gacgctggca
    38161 aaagaaatct taacattttt aacgggtatc tctacccgtg acggagataa attccctgtg
    38221 cttattagtg caatcagcaa ggtatctgaa agcgaacaca gaggactgtt aaatgttatc
    38281 acagaattac ggcaagagaa tacaccgatt gcgaaccata tcgcaaatca tattgccagt
    38341 tttacaaatt atgattttgc acatctgctg ttttcggacg ggacagtaga aaatacaatc
    38401 agtctggata accagctcaa tatcattcaa gtagctgacc ttgtactgcc agataaagat
    38461 accactttta acgaatacac gaccattgaa ttattatcgg tggcaatgct gattgtgatt
    38521 agtacctttg cccttgattt tatccattca gacagaagta tttttaagat tgtagacctt
    38581 gacgaagcgt gggcgttctt aaatgtggca caaggcgaaa ccttatcaaa taagctggtt
    38641 cgtgctggac gagctatgca ggcaggcgtt tattttgtta cacaatcttc tggcgacgtg
    38701 tcaaaggaaa gtctgaaaaa caatatcggt ttaaaattcg ctttccgttc tactgacatc
    38761 aacgagataa agcagacctt agagtttttc ggtatcgaca aggacgacga gaacaaccag
    38821 aaacgcttgc gtgatttgga gaacggacaa tgcttattgc aggacttata cgggcgtgtc
    38881 ggtgtggtac agatacaccc cgtttttgaa gaactgctac acgcctttga tacaagaccg
    38941 cccgtacaga gaaatgaggt ggagtgatga aagaaaggat aaaaggtgcg ttcacaaaaa
    39001 agaagatttt ccactttctc aaaatggctc tgttcgtagt ggcactctcc cttatcctgc
    39061 tttcacttct ggggacggta gctcatgcga cggggcttgt ggacgatacc ataaacgtag
    39121 aaaatcttta ctcaaaatac cccctttcca actaccagct tgatttttat gtagataata
    39181 gctggtcgtg gcttccgtgg aactggctag acgggattgg aaaatcggtg cagtacgggc
    39241 tttactgtat taccaacttt gtctggacga taagccttta tttgagcaat gccacgggct
    39301 atgtggtgca ggaagcctat aaacttgatt ttattaacga tatggcagac agtatcggaa
    39361 agagcataca gacccttgca ggcgtaacac agaacggttt ttccagctct ggcttctatg
    39421 ttggtttcct gcttctcatt atcttggtgg tgggacttta tgttgcctat acgggactga
    39481 taaaaagaga aaccagcaag gcacttcacg cagttatcaa ctttgtggtg gtgttcgtgc
    39541 tgtccgcttc gtttattgcc tacgctcccg actacatcaa aaagataaat gaattttcat
    39601 cagatattag taccgcttca cttgatttgg gaacaaaaat catgctccca aactctgaca
    39661 gcgagggcaa ggacagcgtg gacttgatac gggacagctt attttctatt caagtggaac
    39721 agccgtggct actcttacaa ttcgggaaca gcaacgcaga ggaaatcggg acaaaccgtg
    39781 tagaagctct tgtatcggca agtccagagg tcgaggacgg aaagaccaga gaggaagtcg
    39841 tgaaaacaga aatcgaggac aacgacaaca acaatctgac gataccgcag gtggtaaacc
    39901 gcttgggtat ggtgttcttc ctcctgttct tcaacttagg gataacgata tttgtattct
    39961 tgcttacggg catgatgttg ttcagccaga tactttttat tatcttcgct atgtttctgc
    40021 ctatcagctt tttactttcc atgataccga gctatgaaag catggcaaag caggcaatcg
    40081 taagggtgtt taataccata atgacacggg caggaataac gctcattgta acggtggcgt
    40141 tcagcatttc cagtatgttt tataatatat ccacggacta tccgttcttt atggtagcgt
    40201 tcttacagat agtgtgtttc gcaggtatct acatgaagct gggcgattta atgagtatgt
    40261 tctctttgaa cgctaacgac agtcaaagca tgggacgaag aattttccgc agaccatatc
    40321 tatacttggc acatagggct aggcgtatgg aaagacggct tgctggtgcg gttactgctg
    40381 gcggtgttgc tggtgcggtg gcaggtagca cgatttcaaa cagaaaaccg acaaggaaca
    40441 cagctacaaa agacagtcgg ggcaatacct ctacttccag catgggacag cgtgcaggct
    40501 caaaggtggg tgctgtctta gatacgaaaa ataaagtgaa agacaaggca aatgctgtca
    40561 aagaaaacat caaggatatg ccaacacaga ccgcttatgc ggtgtattcc gcaaaggaaa
    40621 aggcaaagtc cagcgtgtcc gactttaagc gtggcatggt acaggaacag cagtccagac
    40681 agtcggggcg tttggaaaag caggaacagc ataagaaaaa tatcgctgac aagcgtatgg
    40741 agcttcaaaa ggcacaagaa gcaaggcagg cacagcgaaa ggctgacgga tcagcgacaa
    40801 cgggagctac ccgtccccat gagcgaccag ccacagcttc aaagccgagt gcggaaaaaa
    40861 tgcaggaagt caaacgccct gccatagctc aaactccgaa agcaagtgag ccagtcaaga
    40921 caaatgttat caaagagcgt ccgttatctt ctggtgcttc tgataagaaa gcgacccagt
    40981 cggcacagat aacacatagg caaaatgtag aaaaagtggt atcgcaggaa acacgccaga
    41041 attacaccaa agaccgtagg acaaaggttc agcagaccac agagaaaacc cgtaaccttg
    41101 taacgaagaa aggacagaag aaaaaatgaa actgaaacat atcgctctca ttggcagtct
    41161 gtttcctatc ctcttttctc tggtgctttt ctttggggtg ttgattagtg cggacagcga
    41221 cgacgagaac agcaattttt cttctggcat tacgggtatg aacttatccg cagaagtctt
    41281 gaaacatcag cctatggtag aaaaatacgc cagagaaaac ggtatctccg agtatgtcaa
    41341 tgtattgctt gctatcattc aagtagaaag tggcggtacg gcagaagatg ttatgcagag
    41401 ttcggaaagt cttggtttac cgcctaattc cttagataca gaaagttcaa ttaagcaggg
    41461 gtgtaaatat tttgcgtccc tgctttcttc ctgcaaaaat caaggtatcg acgatttgaa
    41521 tgtagcgata cagtcttata actatggcgg tggctatgtg ggatatgtgg caggaaaaga
    41581 gaaaaaacac acctttaacc ttgcagagag cttcgcccgt gaaaaatcgg gtggaaagaa
    41641 agtaacctac accaacccga tagccgttgc gaagaacggg ggctggcggt atggctatgg
    41701 aaatatgttc tatgtggaat tagtcaatca atatttgacc gtggcacact ttgataatgc
    41761 gacggcacaa gcgattatga atgaagcgtt gaaatatcaa ggctggaagt atgtgtatgg
    41821 tggcagtaac cccaacactt cctttgattg tagcggactt gtgcaatggt gctatggaaa
    41881 agctggtatc tccttgccga gaaccgcaca agcacaatat gacgctaccc aacatcttcc
    41941 gctttcgcag gcaaaggctg gggacttggt gtttttccat tctacctata acgctggttc
    42001 gtatgtaacc cacgtcggca t
//
LOCUS       DBGJFU010000021        41671 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.021, whole genome shotgun sequence.
ACCESSION   DBGJFU010000021 DBGJFU010000000
VERSION     DBGJFU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.021"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            53..1597
                     /locus_tag="ACOIAS_001087"
     CDS             53..1597
                     /locus_tag="ACOIAS_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2700806.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKRNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
     gene            1773..2141
                     /locus_tag="ACOIAS_001088"
     CDS             1773..2141
                     /locus_tag="ACOIAS_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2700807.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            2143..2868
                     /locus_tag="ACOIAS_001089"
     CDS             2143..2868
                     /locus_tag="ACOIAS_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700808.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            2862..3626
                     /locus_tag="ACOIAS_001090"
     CDS             2862..3626
                     /locus_tag="ACOIAS_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2700809.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            3848..5098
                     /locus_tag="ACOIAS_001091"
     CDS             3848..5098
                     /locus_tag="ACOIAS_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2700810.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNIDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            5291..6175
                     /locus_tag="ACOIAS_001092"
     CDS             5291..6175
                     /locus_tag="ACOIAS_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGZ2700811.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6534..7151
                     /locus_tag="ACOIAS_001093"
     CDS             6534..7151
                     /locus_tag="ACOIAS_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HGZ2700812.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            7259..9790
                     /locus_tag="ACOIAS_001094"
     CDS             7259..9790
                     /locus_tag="ACOIAS_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2700813.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            10224..10925
                     /locus_tag="ACOIAS_001095"
     CDS             10224..10925
                     /locus_tag="ACOIAS_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HGZ2700814.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            10975..12057
                     /gene="vanS"
                     /locus_tag="ACOIAS_001096"
     CDS             10975..12057
                     /gene="vanS"
                     /locus_tag="ACOIAS_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HGZ2700815.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            12318..13418
                     /gene="vanG"
                     /locus_tag="ACOIAS_001097"
     CDS             12318..13418
                     /gene="vanG"
                     /locus_tag="ACOIAS_001097"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HGZ2700816.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            13415..14221
                     /locus_tag="ACOIAS_001098"
     CDS             13415..14221
                     /locus_tag="ACOIAS_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGZ2700817.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            14239..16377
                     /gene="vanT"
                     /locus_tag="ACOIAS_001099"
     CDS             14239..16377
                     /gene="vanT"
                     /locus_tag="ACOIAS_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HGZ2700818.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            16691..17311
                     /locus_tag="ACOIAS_001100"
     CDS             16691..17311
                     /locus_tag="ACOIAS_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGZ2700819.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(17477..18013)
                     /locus_tag="ACOIAS_001101"
     CDS             complement(17477..18013)
                     /locus_tag="ACOIAS_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGZ2700820.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(18126..18830)
                     /locus_tag="ACOIAS_001102"
     CDS             complement(18126..18830)
                     /locus_tag="ACOIAS_001102"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HGZ2700821.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            19003..20877
                     /gene="asnB"
                     /locus_tag="ACOIAS_001103"
     CDS             19003..20877
                     /gene="asnB"
                     /locus_tag="ACOIAS_001103"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HGZ2700822.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(20969..22405)
                     /locus_tag="ACOIAS_001104"
     CDS             complement(20969..22405)
                     /locus_tag="ACOIAS_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HGZ2700823.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            22731..23312
                     /locus_tag="ACOIAS_001105"
     CDS             22731..23312
                     /locus_tag="ACOIAS_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2700824.1"
                     /translation="MMGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLD
                     TAAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPG
                     EISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVA
                     EIYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            23351..23929
                     /locus_tag="ACOIAS_001106"
     CDS             23351..23929
                     /locus_tag="ACOIAS_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2700825.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            24015..24644
                     /locus_tag="ACOIAS_001107"
     CDS             24015..24644
                     /locus_tag="ACOIAS_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2700826.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            24634..27393
                     /locus_tag="ACOIAS_001108"
     CDS             24634..27393
                     /locus_tag="ACOIAS_001108"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HGZ2700827.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            27480..29042
                     /locus_tag="ACOIAS_001109"
     CDS             27480..29042
                     /locus_tag="ACOIAS_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HGZ2700828.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            29043..30179
                     /locus_tag="ACOIAS_001110"
     CDS             29043..30179
                     /locus_tag="ACOIAS_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HGZ2700829.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            30243..31319
                     /locus_tag="ACOIAS_001111"
     CDS             30243..31319
                     /locus_tag="ACOIAS_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGZ2700830.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            31297..32535
                     /locus_tag="ACOIAS_001112"
     CDS             31297..32535
                     /locus_tag="ACOIAS_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HGZ2700831.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            32589..33677
                     /locus_tag="ACOIAS_001113"
     CDS             32589..33677
                     /locus_tag="ACOIAS_001113"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGZ2700832.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            33705..34496
                     /locus_tag="ACOIAS_001114"
     CDS             33705..34496
                     /locus_tag="ACOIAS_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HGZ2700833.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(34611..35165)
                     /locus_tag="ACOIAS_001115"
     CDS             complement(34611..35165)
                     /locus_tag="ACOIAS_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGZ2700834.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            35361..36563
                     /locus_tag="ACOIAS_001116"
     CDS             35361..36563
                     /locus_tag="ACOIAS_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HGZ2700835.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            36620..37600
                     /locus_tag="ACOIAS_001117"
     CDS             36620..37600
                     /locus_tag="ACOIAS_001117"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HGZ2700836.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            37848..38801
                     /locus_tag="ACOIAS_001118"
     CDS             37848..38801
                     /locus_tag="ACOIAS_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGZ2700837.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            38791..39747
                     /locus_tag="ACOIAS_001119"
     CDS             38791..39747
                     /locus_tag="ACOIAS_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGZ2700838.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            39744..40499
                     /locus_tag="ACOIAS_001120"
     CDS             39744..40499
                     /locus_tag="ACOIAS_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2700839.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            40591..41556
                     /locus_tag="ACOIAS_001121"
     CDS             40591..41556
                     /locus_tag="ACOIAS_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2700840.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT        16190 a         4296 c         7214 g        13971 t
ORIGIN      
        1 gatgtactta tttgctaata aaatagaggt gttttaatgg gaggaaattt ttatgaggaa
       61 tttacaaaaa aaattgatgt tttttttatt tatttgtata attttacaaa ttacatacac
      121 aacaccagtc tattctttat gcaaactcta taaaaacaac tccataaata actttgagtc
      181 tattgtgtct aataatcagc ttattagcaa acatgcaata caaataatac ttattatggc
      241 tataatatgt gtaatactta tattctatat aatatatgat aaattaaatt ttaaaattaa
      301 attgcaaagg atagcatata cagataatct tacaggagca aatactatag ataaatttgt
      361 aattgatgct aacaaaatac tatgtaaaaa tactcaagta aagtatgctt tattatatat
      421 agatattgat aaatttaaat atataaatga tttatttgga tatgaagttg ggaatgaaat
      481 tttgcgtaat ttaacaaaga ttattaagag gaatatattt gaagaggaaa tgtttgcaag
      541 aatatctgcg gataatttta ttataattat gaaatatata gaagaagaag atataacaaa
      601 gaggttaaaa actatatttg aagaattaga cttgtttaat aataatcaag aagaaaaata
      661 taagttagtt ttaagttgtg gaatctattt tatacttcca gaagatagag atatcaactc
      721 aatcatagat agggctaata tacctcataa aatggcaaag ggaggacata aaagttcata
      781 tgcattttat gataataaga ttcatgacca agagataaaa gaaaaagaga tggaaaatac
      841 aatgttttca agtctagaaa ataaagaatt tattatatac ttacaaccta agatagaact
      901 taatacagga gagatacaag gttcagaagc acttgttagg tggaaacgtc ctgataaagg
      961 gctaatacct ccaaatgaat ttatcccatt ttttgaaaga aacggttttg ttataaattt
     1021 agatttatat gtgcttgaag aagtatgtat ataccttaga aaatggattg atgcaggaat
     1081 aaatccagtt actgtttcag ttaatgtttc aaggatacat ttatattgca ataattttat
     1141 agaaacatac aaaaatataa ttgataaata caatatacct gcaaagtata tagaacttga
     1201 attaacggag agtatcatat ttgataattt tgatatttta attgatataa tgaataattt
     1261 aaaaaaaata ggatttttaa tatctatgga tgattttggc tcagggtact catcattaaa
     1321 tatgttaaaa gaaattccta tggatatctt aaaacttgac caaaaattta ttatggaaac
     1381 ttataatagt aaaagaagca aaataattgt tactaaagta atagaaatgg caaaagagtt
     1441 gggtatgaaa gtcatatcag aaggagttga gacagaagaa cagtttaaac ttttaaaaga
     1501 agtaaagtgt gatatggcac aaggatattt gtttggaaag ccaatgccaa ttgaagaatt
     1561 tgaacattta attgtatcta acttggtaag agggtaatta atctgtaata taaagaattt
     1621 aataaattgt gttaaaaatg gactctaatg tagtgttcat tttttttgta aaatttttta
     1681 tacttttact aaaaagtaat tgacatatac tttgtattat tatattattg tattaacaac
     1741 ttaatacaat aatataataa ggaggcatga acatggaatg ggaacttgat aataacaaac
     1801 ctatatatat acaattagta gaacatttaa agttaaaaat aatttcaggg gaaattaaaa
     1861 taggatctaa attagaaaca gtgagagcac tagcagaaga tgcggaagtt aatccaaata
     1921 caatgcaaaa ggctctaaca gaacttgaaa gacaaggttt agtttatagt cagagaacta
     1981 aaggaagatt tgtaacagat gataaggaaa aaataaaagc tatgaaagag gaaatagcta
     2041 atgtggagat aaatacatta aaagtaacct tagaaaagct tggatatgat agagatgaaa
     2101 tgctaaaact tattacagaa aatctgaaag gagaattata aaatggataa aaatataagc
     2161 aatgtttctc aagatggaat agttgaattt aaaggtgtaa acaagagtta tggaactaag
     2221 aatgttttaa aaaatattga tttaaatata ccaaagggaa aaatagtcgg gctgttagga
     2281 cctaatggaa gtggcaaaag tacaatgatt aagctaatga atggattatt acagcctgat
     2341 aatggtgaaa ttatgataaa tggaatgaaa ccatccatag atactaaaaa aatagtttca
     2401 tatttaccag aaagaactta tttaaatgat tggatgaagg tatcagattt acttaagttt
     2461 ttctatgatt tttatagtga ttttgatgtt agaagagcaa atgagatgat taaaagctta
     2521 gatattgatg taaatgaaaa gctaaagaca atgtctaaag gaactaaaga aaaagttcaa
     2581 cttattttag taatgtcaag aaatgcgagc atatatatat tggatgaacc tataggtggt
     2641 gttgacccag ctgcaaggtc atatatactt aagactatat taaaaaatta ttctgaagat
     2701 agtactttac tgattgctac ccatttaata agtgaaatag aaaacatatg tgatgagata
     2761 atattcatat caaaaggaga aatagtgtta caaggagacg ttgaagcaat aagagaagaa
     2821 aaaggaaaat ctatagatgc attatttagg gaggaattta aatgttaggt aaattattaa
     2881 aatatgagtt aaaagcaagt ggaagaatat ttataccgtt atatatagcc atattgatag
     2941 tagctgtatt taacggaata tttatgaata caaatatact tcaggtacag ggaataggaa
     3001 tactagtatt aacaagtctt tttatggcat taggagtttt aactattgta gttacaattc
     3061 aaaggtttag aaaaaatctt ttgggggatg aaggttattt aatgtttact cttcctgtta
     3121 gcactagttc acttatatta tctaaatgta taacagcact tatatacgct gtacttagtt
     3181 ttatagttgc agtattcact tttggagttc ttatgctttt tggtacttct ggaattttac
     3241 tacctgaaat tttagatttg tttaacacta gttttaaatg gatttctgaa aactttttag
     3301 atattttatt attagtagta gtaatgttta tttcatattc atcatttatt ttgttattat
     3361 atacatcaat ctcaatggga caattaccaa aattcaataa acatagaaac atagtggcat
     3421 ttgcttcttt tatagctata aatatagtaa tatcaattgt tggagatgca gttggaagca
     3481 tcttaccaaa tgaagataca aatatggtat atcatcttta tcagagtcct agttttatgc
     3541 ttgcaatact tggtagtctt gtagttgcaa tagcgttatt tttcgctaca aaatttatat
     3601 tagataaaaa attaaattta gaataaatgt ttttatatgg agccatcttt tgagtaatgt
     3661 taaaagatgg ctcttattat ttatgtagtt aattcatgta tataatatga atagtgtttt
     3721 atttctagtt aaatatatac atatgttgta taatgatatt gcgtgtaaaa atttgtaaga
     3781 ataactatac actttatata agttatatca agaaaattta aaaatatatg gatttgaggg
     3841 agaagatatg aaaagttata caaaggctaa gtggagtgtt tggggtataa ttacattttc
     3901 atttgtttta gtattatttt taaggatgtc aactgctgtt gtttcagaca atttagcaaa
     3961 tgagttaggt tttaattcta tacaaatttc aaatatagca tccttttgcc tatatgcata
     4021 tgcatttatg caaatacctg caggaatttt gattgataaa tatggagcaa gaaaaattag
     4081 cagtcttgga ataataatgg caagtttagg gtcaatttta tttggtttga ttcaaagtat
     4141 agaattagca tatatttctc gtgtaattgt aggggcagga acatcagtaa tattactttg
     4201 tattttaaaa attcaaggga gatggtttaa taagtcagaa tttgcttctg caacagctaa
     4261 attttcattt gtaggaaatt taggtggagt tcttgctact tttccacttg tgtttttgtc
     4321 tgaacttgtt ggttggagaa attcattttt attaatcggt ataattggtg ttgtaattgg
     4381 atgttttatg tatattattg ttagagatac accaaaagag tatggattca atatagatac
     4441 tgaaccctat gaaaagtcag agaaggttaa tattgttgat ggtattaagt ctgttataaa
     4501 gaacaaatca acatggtata attcaatgat aatgttctct tttgtgggtc ttacaagtgc
     4561 atttataagc ttgtggggag taagatacat aatggacgtt tatggtgtga gtaagagttt
     4621 ttctgcgttt atagtatcat tttttacata tggatttata tttggttcaa taattatgga
     4681 ttttgtattt gctaaaataa ggtctagcaa atttaatata ataaaatttg gggcaatgat
     4741 agatttgttt atatggattg taattgtagt tgtataccag gtaaaaccac caattatagt
     4801 tttaccaata tcattcttta ttatgggatg tatagttatg tcacatctac aggtttttaa
     4861 tgatgccaag tataaaaata aagaaattta ttctggactg gcaactagtg ttattaatac
     4921 ttttgaattt ataggaagtg gaattattaa tttgattata gctatctctt tacaagtaaa
     4981 ttcatataat atagttgatg gatataaaaa aggttttgta gtatttatag tactaagtat
     5041 tataactata gtatcatctc atataggggt aaaaaatgat gattttaagg ctatatagtg
     5101 ttttattaat agtttaattt agtattatat tttgcatata tatataacaa atagtttgca
     5161 ttttcctaag atgtataatt ggaatataaa tcttgtttac tatgtacaaa tggtattgta
     5221 ttatattaaa tagaaaataa gaactttaaa ctataatatt ggttctaaaa tataaggtaa
     5281 ggagtttttt atggatacga cgaaaataga tgatataagc aagttagatt taaataattt
     5341 agatattaat aagatggata tgggaggtat aatggaaagt ctattagaat gggctactac
     5401 aagcggagta aaacttataa taggcatact tatactttct attggattta aaataataaa
     5461 aaaatttgta aaccatgtta tgttattact tagtaaaaga gatattgatt taactttaag
     5521 aaggtttttg aagtctcttc ttttgagtgt acttaaagtt gccgttataa tcattgttct
     5581 ggaatattgg ggaatgagtt tgtccagttt tgctgctgtg atagcatcag caggggtagc
     5641 aataggtttg gctttacaag gtagtctatc aaattttgct ggaggattta ttatactatt
     5701 gattagacct tttaaagtag gagattacgt agaagcagca ggccatggag gaacggttga
     5761 acaaatagga cttttttata cacaattggt tactccagat aataaacaga tacttatacc
     5821 taatggttct gtatcaaatg atagtctaat aaactattca gcaaagaata ctagacgagt
     5881 tgatttgact tttagtgtag ggtatgaaga tgatatacta catgttagaa gagtacttaa
     5941 agatattgtt aataatcata aattaataat aaatgaacca gaaccattta taggagtagt
     6001 agaacatgga gataatgcta ttaaatttgc aactagagtt tggtgcaaga cagaagatta
     6061 ttggacaatt tatcttgatt tactagaaga ggttaaagtt aggtttgacg aagaaggaat
     6121 tacgattcca tatcctaaaa tggatttaac ggtaaaagaa ttaaataaaa tttaattttt
     6181 ttatgtagtg tttaaattta agaatattat atagatatat ttataagatt tgtgaaaaat
     6241 taataagtcc tgttattttg taagtgaaaa tataaaaaat gggagtgtct caaaatgaac
     6301 tttttagttc atgaggcact ctttttgtat gaaatcaaat atatttttat tatttcaaca
     6361 ataaaaaaga ggtctgtttc tattttgaga caatattatt tttatgtaga tttaatctta
     6421 atgagtataa agttttaaaa ctactaaaat aggaggaatt ttaatataaa agggtaaata
     6481 aagggtttag agggcataat atagaataaa ttaattattt aaggagggtt tttatgtgca
     6541 aaaaaatgta tttattagtg gtaggtatac ttatagcagg tttattaact gcatgtaatg
     6601 ggagtccaac taaggattca gaacaagcac aagataatac aaaaaaggag gcaaatgtat
     6661 caaataacgc tttaaaagat gaaaaaaata atgaaaattt aatggaacaa gattttaaag
     6721 tgccatacac agatgcaata aacatattta aagacaaata caaagatgct gatatagtag
     6781 atttaagtct tgagagagat ttaaataaat ttgtttatac tgtggaaggt gtagatgata
     6841 ataatgaata taagatgaaa attgatgcta atacaaaaga tgtattagaa gataaaacag
     6901 aaaagttgga ttcagaagat ttaaatggag tcgctagaaa agaaaaatta gattttaatg
     6961 acataatgac acctcaacaa gctatggaaa ttgcattaaa agaacaaaat ggaatggtta
     7021 aagagtggag tttagataaa gatttagatg ttacttttta taaaataaga atagataaag
     7081 ataaaaatga atatgacata aaagtagatt ctaaaaaggg aactgtattg aaggtagaaa
     7141 aagaagatta gaagatttaa actatcttta aaataatttc ttttttgaat aaaatttgtt
     7201 taaaatataa caagtagggt ataaagagac taaataacta ttttaggagg agtacggtat
     7261 gtcattgaaa tcgctaaaaa taaaagaaaa tttatattgg gtaggttcgc ttgaccctga
     7321 tttaagagta tttgacataa ttatgtatac accttatgga actacttata attcatatgt
     7381 attaaaagga acagaaaaaa cagttttatt tgagacagtt aaagataagc attttgacaa
     7441 ttatattgaa agattaaatg atttaaatat agattttgaa aaaatagact atattgttgt
     7501 aagtcatact gaaccagacc atgcaggaag cgttgaaaag cttttggatt tagctaaaaa
     7561 tgcaaaagta gtagcttcag aaacagcaat taaatatctt aaggaaatcg taaacaaaga
     7621 ctttgaatat gttgcagtta cagatggaga tacattatca attggggaca aaactttaga
     7681 atttttctct gtacctatgc ttcattggcc agataccata tacacatata taaaagagga
     7741 taaaactctt gtaacatgtg attcatttgg tagtcactat agtaatgata agatagtaaa
     7801 tacacttgat gaagatgaag aaaaggatta cttggatgct ttgagatact attatgattg
     7861 tataatggga ccatttaaac catctatggt aactgccata gaaaagataa aagatttaga
     7921 tatagatact gtatgtccag gtcatggtcc agtattaact gaaaatccta gaaaaattat
     7981 agacctttat tacaactgga gtgtaaatga acaaataaag ttagaaaaag aagttacaat
     8041 ttgttatgtt tctgcacatg gatatacaaa gattatggca gaagctataa aagcatatat
     8101 tgaaaaaaat agcaactata aagtaaattt atttgatgta atagagcata agcaagaaga
     8161 tattttggca aaaatagcag tatcacaagg tgtattattt ggaacaccaa caatattagg
     8221 agatgcgtta aagcctatat gggatatatt aatatcgtta aacccagttc ttcatggtgg
     8281 aaaagttgct tcagtatttg gttcatatgg atggagtgga gaaggtatag aaaatgctat
     8341 ggagagaata agtcagctta gaatgacagc agtaaaacct tttgctgtta actttaaacc
     8401 atcaaatgaa gagattgata aattacattc ttatacaggt aaattcctag ataagttaaa
     8461 ttctactttt ggaagtaaaa agaaaactaa gaaatttaaa tgtgtaattt gtaatgaagt
     8521 ttttgaaggt gatagtgctc ctagtgtatg ccctgtatgt ggagctaagg aagaccaatt
     8581 tatagaagtt gaagaagatg aagttacttt tagaaaagat actgatgaat atttcgtaat
     8641 agtaggaaat ggagcagctg gtttttatgc agcagatgct ataagaaaaa gaaataaaac
     8701 ttgtaagata actatgattt ctaatgaaga tgagttgact tactatagac ctgcactttc
     8761 tgatggtata aatgaagaac ttggttcaga tttctacatg gaagataaag attggtatga
     8821 taaaaataat atagtagtaa tattaggaac gaatgtagat aaattagatg aagtgaataa
     8881 aactattata gtgaatgatg gagctattaa atttgataag cttgtaatag ctacaggaag
     8941 tagaaacttt ataccaccaa ttaagggtca tgatttagaa aatgtgttta ctcttagaaa
     9001 tataaaagat ttatatagtg taaaagaagc actagaaaaa tctaagaaag ttgtagtgat
     9061 tggtggcggt ttattgggac ttgaagctgc atgggaattt agactaaaag gattagaagt
     9121 agtagtagtt gaagccatgg atagcatact atcaaaacaa cttgataaag aaggcagtaa
     9181 aatattagaa caatgtgtaa gagatacagg aatagatgta agattgggtg tagcagttga
     9241 tggtattgaa ggtgatggaa aagctcaaaa agttgtattt aaagatggag atagtgttga
     9301 ctgtgatatg gtagtatttt caattggtgt aagagcaaac actcaaatgg ttcaggatac
     9361 atctgtaaaa attgacagag gtattgttgt agataagaca ttacagacaa atgttaaaga
     9421 tatatatgct tgtggagatg ttgcacaagt gggaaatatt agtttagcaa tatggccatc
     9481 ttcagtagaa atgggaaaaa tagcaggggc aaatgcaagt ggagataatt tgacatttga
     9541 aagtgaagta tatccagttt cattagatgc aatgaatgta aaagtattta gtattggtaa
     9601 tatacaaaat tttgataaag aaatttcatc aaaagatgaa ggtcaaagaa tatataaaaa
     9661 attatttatg aaagatggtt cattagttgg agctatactt ataaatgatt taagctgtac
     9721 tgttaaattg ataagattaa tatctgaaaa aggtgatttt gaggatatta tgaaggctga
     9781 tatactataa tttaaataca ttaaaataaa aaggagttgt cttaaaattg atttttttct
     9841 aatcaaattt taaattcaac tccttttttg aatttttata taaaataaca tggaaatttt
     9901 agtaatgtct gtggttacat acataggttt aattgttttt tatatggatt gtctctggta
     9961 taataaattg aattgtgttt gactgataga tatttttatt gaaagttaat acctgttatg
    10021 cgataataag gacgtgttag gggagttttt ataataacaa ttaatctcag tttttgtgtt
    10081 ccaatagtag catcaacaca ttctgatgta gtagttcttt tcttataaaa attagttaca
    10141 aatatgcttg taattatttg atatgtaaat ttaccaatat tataaatgat gacctcgata
    10201 ataaggagag tgaaaaataa tctatgaacg aaaatatttt gattgtggat gatgaaaaag
    10261 aaatagcaga tttaattgaa gtatatttaa aaaatgaagg ttttacagta tacaagtttt
    10321 atacaggtaa ggaagctcta gaatgtattg aatctaaaaa tttggatatg gctattttag
    10381 atattatgct tcctgatatt gatggatttc atatttgcca gaaaattcga gaaaaatact
    10441 attatcctat cattatgtta acagctaaaa ttgaagatat agacaagata atgggtttaa
    10501 ccataggtgc tgatgattat ataacaaagc catttaaccc tttggaagtt gttgcaagag
    10561 taaagacaca attaagaaga tatatgcgat ataataattc ttatgaacaa cagtcgataa
    10621 ttgttaatga gtatgatatt aaaggattga ttatcaataa agaaacacat aaatgtagtt
    10681 tatttgggaa agaagtagca ttaacaccga ttgaattttc tattctttgg tatttatgtg
    10741 aacatcaagg aaaagtggtt ccttctgaag aattgtttga agctgtatgg ggagaaaaat
    10801 atcttgataa taataacaca gttatggctc atatagggcg attacgtgaa aaaattcatg
    10861 aaccttctaa aaacccgaaa tttataaaaa ctgtatgggg agtaggttat acaattgaaa
    10921 agtaaaaata ttaaagtcaa tcctgacttt actcaagtga gaaataaaat ttttatgaga
    10981 acagctttaa tggcatttat agccattatt ttcatatgga ttttatattc ttttatattg
    11041 tatggtaagt tttctatctg gatagtatca ttctttgaaa atgcttttcg tatggaatat
    11101 aaagctgcat tgaatttata tcagcagatt ttccgtaata gtatggagct cttcatgata
    11161 ctagctatgg tgattgtgtt ttttataata ttccgtgttt atttaaattg gtttacaaaa
    11221 tattttagtg aaataaatca aggtattgac tctcttatca aagaagatgt tggagaagtg
    11281 gctttatctc cagagttatt ggcaatagaa aagaaaataa actcaattaa acatatactt
    11341 gaacaacgta aatttgagac acaaatggca gaacaacgta agaatgaact tattgtttat
    11401 cttgcacatg atttaaaaac accacttaca tcagttatag ggtatctgac actacttcga
    11461 gatgaaagtc agatttctga agaactgcga aaaaaatatt tatctatttc gttggataag
    11521 gcagagcgtc ttgaagattt aattaatgaa ttctttgaaa ttacaaggtt taatttatca
    11581 aatatatcac ttgaatacag tacagtaagc ttaactcgta tgcttgaaca actaacatat
    11641 gagtttaaac ctactttgat gagtaagaat ttaaaatata cactgaatgt tacaccaaat
    11701 atgatgataa ggtgtgatgt taataaaatg caacgtgtat ttgataactt attacgaaat
    11761 gctgtttatt atagttttga agatactact attgaaataa cagctgtaca ggaggatgat
    11821 tatataaagc ttaaatttat aaatcatagc aatacgattc cagaggaaaa acttgagcgt
    11881 atttttgaac aattttacag gttggataca tctcgtaatt caaacagtgg tagagctggg
    11941 ttgggacttg ctatagctaa agaaatagtg gagcttcata acgggacaat aacagctcat
    12001 agcgagaaca atataattac ctttaaggtt attattccga ttgtaggaaa tttgtaagaa
    12061 tttaagtata aaaaaatagg aaattcttta ggataaataa ataaagagca ctcttgttat
    12121 tgatataatt actgtatcaa aacaagagtg cttttatttt gatacagtaa ttatatcaat
    12181 agaaaaatgt gaaaatatat tatgtggaac agatgcaatt gtaagtgaag gttgattgga
    12241 tttgaaatag aaaaagtatt ttaacatgtt tctgtagatt gtataaaaat aaataaatca
    12301 acaaaggaga atcaaagatg aataataaaa aaattgcagt tatatttggt ggaaactcaa
    12361 cagaatatga tgtatcatta caatcagctt ttgcagtact tgagaatttg aatacggaaa
    12421 aatatgatat tataccaatt ggaattacaa gggagggtaa ttggtatcat tatattggag
    12481 attataataa tatccagaat aacacttggt ttgaaaggga tactgatata attccagtta
    12541 ttgtttcgca gaaccgtgtc aaaaatggta ttattgaatt tttaattcct gatgctgttt
    12601 ctcagaatca tattccaaat agtgctattg aatcatgtga aaatgatatt aaatacacta
    12661 aacttgattt tgtgtttcct gtactgcatg gaaaaaatgg agaagatgga acagtgcagg
    12721 ggttatttga attggcaggt atacaggtta ttggatgtgg tactctttct tcagcattat
    12781 gtatggacaa agacaaatca cataaacttg ttcattcaga aggtattaaa gtaccaaaat
    12841 caatattact aaaatactca gataaattta atgtggctga actgtcaaaa gacttaactt
    12901 atccattatt tataaaacct gtgcgtgcag gttcatcatt tggtattagt aaaatttata
    12961 aaaaagaaga gttacaagta gcagtagact tggcttttga acatgataat gaagtaataa
    13021 ttgaggaaaa tattgatggt tttgaggtag ggtgtgcagt tttaggaaac aatgacttac
    13081 ttgttggaag agtagatgaa atagaattgt caaaaggatt ttttgattat acagaaaaat
    13141 atacattgaa aagttcaaaa attcatatgc cagctagaat tgatttggat tgtgagaaaa
    13201 gaatacaaga gacagcaaaa attatttata gaactcttgg atgttctgga tttgctagag
    13261 tagatatgtt tttaactcca tctggtgaaa ttatttttaa tgaagtcaat acaattcctg
    13321 gctttacttc tcatagtcgt tatccaaata tgatgaaagg tattggactg tcttttgaag
    13381 aaatattgga caagataatt gaggtgtaca taaaatgatg aataatattt tattagataa
    13441 aaataaagaa gataaaatga caaatactat cttattgact aaagataaag tttatgatgg
    13501 gaatctaatt ctagtaaatg cttttttacc agttaaaaca tctgaagata tagatttaat
    13561 tcctgtagat acaaggtttc caagtattct tatgaaacgt gaagctacaa acattttaca
    13621 aaatatttta aaaagtatat gtggaataaa tgaaatagta cctgtaagtg gatatcgtac
    13681 tgcagaggaa caacaagata tatattcaag ttctctgcgt gacaatggaa aagactttac
    13741 taaaaagttt gtagcacttc caaatcacag tgaacatcag acagggcttg ctattgattt
    13801 agggttaaag aaagaagtta tagattttat ctgccctgaa tttccttata atggtatttg
    13861 taatgagttt cgtaaggcag cactacatta tggttttata gagcgttatc agtgtaaaaa
    13921 agaagaaata acaggaatat ctcaagaacc atggcatttt cgttatgtgg gatatccgca
    13981 ttctgagatt atcaataaaa tgaatctcgc tcttgaagaa tatatagaat atattaagag
    14041 attttcgtat acaaattgtt taaaaataga agataaagaa aaaattatag aaatctttta
    14101 tgtgcctatt catactaaag aagaagtgag aatctctata cctgaacaat gtgtatatca
    14161 aatatcgggt aacaataagg atggttttat tgtgacattt tggagaaata gaaaggacta
    14221 agaaatggag gaacaagaat gattaggaat aaatcttatg cagggcttga ttattttagg
    14281 attatagctg cattacttat aattgcaatt catacatctc cattattatc atatagtaaa
    14341 atggctgact ttgtgttaac tagtataatt gctcgtattg ctgtgccatt cttttttatg
    14401 ctatcaggct tttttctaat ttcaacatat aattataatt gtaataaact gaaatctttt
    14461 ctaaaaaatg tattatttat ttacgggata gctattgtaa tttatattcc cttaaatatt
    14521 tacactggat atttttccat gaataattta gtgcccaata ttattaagga cattgtattt
    14581 gatgggactt tatatcattt atggtatctt cctgcatcaa ttattggtgc aagtattgca
    14641 tactttcttg taaaaaaact aggtttaaag tactcactag taatcactat tgttatttat
    14701 gtaattggta tgtttggtga tagttattat ggttttttag aaaaaatacc actattaaag
    14761 aatttatatg ggtatatttt tgaagtatca gattatacac gaaatggtat attttttgca
    14821 cctgtatttt tcatacttgg tggaataata tctaataaat ctaattatat gtcaactaaa
    14881 aatagcttga ttggatttct tatatcattc ttacttatgt tatgtgaggg aatgttttta
    14941 cataaactag gggtacagcg tcatgatagc atgtatatta tgttattgcc atgtatgtat
    15001 ttcttattta cagccttaac atttttgaaa ggttctcgag ctaggttatt gcgaacatca
    15061 tcattaatta tttatattat acatcctatg atgattgttg ttattcgtat gttttctaaa
    15121 atattcggaa tacaaactgt cttggttgac aatagcctta ttcattatat agtggtaagt
    15181 atagcatctt ttatagcttc tataattatt atattgattc ttaaacctct aaaaaaagac
    15241 ttgaaaggag aacataaggt aaataccaat agagcttgga ttgaaattga tttaaataat
    15301 ttggaacata atgtaaagat gatacaaaaa gctatgccag aagattgtga attgatggca
    15361 gttgtaaaag ctaatgccta tggtcatagt gcttttgaag tagcgacttg tgcaaaccaa
    15421 ataggtgtaa aagcatttgc agtagcaaca atagatgagg ggattgattt acgtcgatat
    15481 ggaatactag gagaaatatt gattcttgga tatactaacc caataagggc aaaggaactt
    15541 cataaataca atcttacaca gagtgttatt gaccttaatc atgctatctc tttaaacaaa
    15601 cagaaataca acataaaatc acacattaaa attgatacag gtatgcacag actgggtttt
    15661 gatgttaatg atgtgaggtc aattttaaag gcatttaaat taaaatactt agatatatgt
    15721 ggcatattta cacacttgtg tgtttcagat agtctgaaag atgaagatgt agactttaca
    15781 aataaacaaa ttgaatgttt ttataagtta attgattttc tgttaaaaaa aggtataaaa
    15841 attcctaaag tacatattca aagtagttat ggttttttta attatcccaa tcttaagtgt
    15901 aattatgtaa gaattggaat tgcattatac gggatattaa gttttccaaa tgatacgact
    15961 aaattgcaac ttgatttacg acctgtttta tccttaaaat cacaagtaat tcttattaga
    16021 aaaatacaaa aagatgatag ttttggatat ggaagagtct ttattgcaag cagagatagc
    16081 gtaattgcta tattatctat tggctatgcc gatggtatac ctagaaattt atcctgtgga
    16141 aaaagttacg taataataaa tggttgtaga gcagcaatag tgggtcgaat ttgcatggac
    16201 caacttgctg ttgatattac agatatacct aatgtagaag ttggtaatac ggcaataata
    16261 ataggaagag ataatttatc tgaattatct gtttctgaag ttgcaaataa ctcatgtagt
    16321 atatcgaatg aattattgag cagagtagga agaaggttaa atgttataaa gaagtaactc
    16381 aattaaatag taatatttgt tgtactgtgt aatttatttt actctgacta gacattttta
    16441 gtaatattat gtctaatata tattttttat aacttacatg acaatttaag ttataaaaag
    16501 gtgatgtttc agatttttat tttgaaacat cacctttttt gttataatat ataataaaat
    16561 aattatacct taactgtaaa cttatataga ttatttactt tttaatatta gattcatttt
    16621 ttgagattgg tatataataa acatatagag tatataataa tagttaaagt taaaaaaggg
    16681 gaaaagcaat atgcagtata tacaagaact acttgatttg actcaaaatt attgggtgtt
    16741 agccacaata atcggcttat taagtgcatt tatagaaagc tttataccag ccttaccact
    16801 tgttgctata gttacagcaa atgctgcaat acaaggatta tttattggat gtttgttatc
    16861 atggattggc tcaggacttg ggacaacttc attattctta ttaataagca gatttaatga
    16921 tagtaagctt tttaataagt tgagaaatag taaaactgaa aaagcaataa gttggatgga
    16981 taaacaggga tttaagttat tatttatagc gtatgcctgt ccatttatgc ccggttgttt
    17041 agttactatc gcatcagctt ttggtaaaaa agatttaaaa gattttgtgc cagcaatgtt
    17101 ggcaggaaaa tttgtaatgt ttatagttat aagttacgtg gcaagtgata tagaaggttt
    17161 tataacaagt cctttaaaga tagcatcatt tatattattg gtgtttttgt catggaagat
    17221 tggaaataga gtaaataaaa atcttgaaaa tcataattat gatttccatc ataaaaaaca
    17281 tcacaatgat aaagatgata aaatgattta gcttgtacga aataaaacgt atcttaaaat
    17341 gatttagtac taaattattt taagatacgt tttatttttt gtattaaggt acaaaatttt
    17401 tagttctttt gtaaactaaa atatataaaa cttatttata cttagttcaa aatattgtta
    17461 tttatttttt ctacaactat aataaaatat aaaattgata tccaatcaaa aaaataacta
    17521 tgaaatttaa tagcttagat atgaattttt ctgtttttga gttagttttt ataggtagta
    17581 aagaaatgtt tatagtaatg ggataaaaca attcaatacc ttctttagta ataaggtcaa
    17641 gacacaagtg agatagatat cctattataa acccactgga caaacatgag aaaactatat
    17701 tattgttagt gaagattgtc agagatttaa aaaaactata aataagtaat aaggcaatta
    17761 gactgtgagt aaaccctcta tgtctaaacc tacttccaaa taatttgtat attaacataa
    17821 attttttact tatgtaagaa cctctcatat ctatatctgg aaataaagaa cctaaatatg
    17881 caaaataaat ataaattata attaatacta ttttatatgc aatatcgtat ttaactaggt
    17941 acagattgtt tataaagggg agtatcaaaa gtgcgaatat ataacctccc ctttcatgtg
    18001 tttcttttat catataaaaa tcctcctgaa tcgtcactat aaacattata ttggtaaatt
    18061 tagactatct tttattatat tatatagcaa acataagttt ttttcaaaag gattttaata
    18121 tttaattaat cttgagattt taaatttttg taattttcta aagcaccatt ccaatttact
    18181 acattaaacc agttgtcaat gtattcattt cttctatttt gatattttaa atagtaagca
    18241 tgctcccaaa catcaagtcc tattatagga gttaggtttt tacttacagg gctatcctga
    18301 tttggagtag tcataataga taatttccca tctttagtag ctacaagcca agcccaacca
    18361 gaaccaaaga catctaaagc agatttttgg aactcttgct taaatttttc aaaagaacca
    18421 aagtctctat caatagcttc ttttaaagat tcagaaggta tggttttttc tggcgtcatt
    18481 atatcaaaaa agaatttatg attataagct ccacctgcat tatttcttac agttgtagca
    18541 atatctttag gtaaagaatc taaattttgc aataattcac ataaagaata attataaagc
    18601 tcaggatatt tttcaagagc agcatttaat ttatcaacat aagcttgata atgcttatca
    18661 tgatgcagtt tcattgtttc tttatctata taaggttcaa gtgcatcata tgcataaggt
    18721 aatggtttaa ccttaaattt gttattttca ggtgtaaaag caaatgaagt tatgcactgt
    18781 gagattataa ataaagacat aataactggt attaatattt ttttcttcat aaaaatcacc
    18841 tcctaatata gtatttctag aaataaaaaa aatatgaaat attaacagat aatttataag
    18901 aatttaatag caatttcatt ttaaattgaa taagttttaa agccaaagac aaaactatga
    18961 aatatagaat aagactaaag gacagaaagg aattttgatt atatggacgg caatatttca
    19021 atttattata aaggtgatat atctaaaaaa gaagatatta aatttaaaat aaaagaatct
    19081 gtaaataata caagactttc tagtagaata gagtataagg atgaggaaaa aacttttgat
    19141 tttgaaagat ttaatgtaat tgatgctatt tatggaaata aaccatttga aaaagatgga
    19201 aaagttattg tttttaatgg aaatatatat aactatcatg aaataaaaga agaattaatt
    19261 aaaaaaggac acaattttac tacaaatgat gatattgaag tcttattggc atcttatata
    19321 gaatttggta agaattgtgt acataagatt aaaggaatgt tcaactttat aatctatgat
    19381 agagaaaatg aaagtatatt tggagcaaga gatttatttg ggataaaacc actctattat
    19441 ataaataagg aaaatgcaat aattttttcc tcagagtata agtttttact tgaatatatg
    19501 aaaaatttaa atataaatga aagaagctta caaagttatt tttcatttca atatgtactt
    19561 cctgaagata ctatgataca aggaataagg ctgataccag caggtcacta ttttagggta
    19621 gaaaatggta tactatcttt aaagagatat aataaacttg agtttagaag tagtacaaaa
    19681 tttttttata ctaaaaatca cttaggcaat agagatgtga acgaggatga tataagaaat
    19741 atagtagtag attcaatagt aactcatatg gaagaggaga aagaaattgg aacatttctg
    19801 tctggtggaa tagattcatc tattataact actgtggctt cacaaattaa tccaaatata
    19861 aaatcttttt ctgcaggctt tagtgtcaaa ggatatagtg aattagaagt agcaaagaaa
    19921 acagctgata agttaggtat agaaaatatc cagattaata taacacaaga tgaatatata
    19981 aaatcattac cgaatgtaat ttattctttg gatgacccta tagcagaccc ttcagaagta
    20041 ggtctttatt tcttaattaa agaggcaggt aaacatgtta aagtagcatt gtctggagaa
    20101 ggagcagatg aattatttgg aggatataat atatataaag agtatagtac tatgaaatca
    20161 gttgtcaatt cacctactta tattaaaagt atattaggaa gagtttcaga acttatgcca
    20221 aacataaaag gaagaaatta tctttataga gctacaacac cattagaaaa aagatatata
    20281 ggaaatgcaa aagtttttga aaacagcgaa gtaaaaagat tcttttttaa gtataaggaa
    20341 aagaatatat atgaatacct gttgtctaat ttgtatagag atgcacaaaa aaataattac
    20401 gattatataa gtaaaatgca acatatagat gtgaatacat ggcttcaagg cgatatactt
    20461 caaaaaatta gcaaactttc tacagctgag caggttgaac ttagagtacc atttttatat
    20521 aaagatgtat ttgatgtagc aaaaaattta agaatggagc aaaaaataaa taagaataat
    20581 acaaaagttt tacttagaga ggcgtttaga gaaatagtcc cagaacatgt tgttcaaagg
    20641 aaaaaattag gatttccaac tccaataaga gtttggttaa aagattctct aggggatatt
    20701 gtaaaggaaa ctatttataa ttcaaatgta gatgaattta tagataaaaa atatgcaata
    20761 aaacttttag atacgcatct taaaggtcat agagataatt cgagaaaaat atggactata
    20821 tttacatttt gtctatggca tcaattattt attgagcata aaaatgtcga gtattaatac
    20881 ttttttcaat attaatcaga gggcaaaagc atgcaatgtt ttcaccctcc tgattattat
    20941 ggaataggat aagtagaatt ttgattgatt agtttactat ctcaataaaa ttattggcaa
    21001 atattttgac aagattactt tcaacagcta aatttaagtt ttgtccatca tcatatccag
    21061 cggaaatcaa tcctagtaag tttccaaaca tatctaaaag agctcctcct gaactaccac
    21121 tagagattgg agctgtaaat tgtatcattt gaaccgtttc aaaatcacga aaagctgaaa
    21181 taattccatc tgaaactgta ttaaaaagac ctagagggct tccaattgca actatttttt
    21241 gacctctcac aggtttttta ctgactttaa caggtattgg cttgcatttt ctatcaacct
    21301 ttatcacagc taaatcataa tctgaatggt attttataat ttggtaagat gtataaactt
    21361 tatcatcatt ttcaaatcta actaaaaatg aatacccatc attgactaca tgaagattcg
    21421 taagtatata accttcatta tttattacta ctccagaacc gctcttaaac ggtttatttt
    21481 cgttattgca cacaacaatc ataacaacag aagctgataa ctttgcaagg tcttcataag
    21541 aaagttctac ttcctcatct tgtacaattt ttttagatgt tagatttgtg ttattaagtg
    21601 tatttttaga gttaggttta ttcgtagtag gagaagtctt attggtattt tcactgtaat
    21661 ttatatttgt aatatgcctt tcaaaaggta tttctaatat atttggagtc ttcctagaaa
    21721 gttcaaatag agtagaaaat tttgaacatt caggatataa atcaagaata tctttatttg
    21781 gatttatata taaaatatca cacccaagtt gtgataaaaa atataagaaa aaaagttctt
    21841 gccttttaat atttccaaag tatactatct ttggtgaaat agtagtcttc accttgttat
    21901 aaaataattt aggtagaaaa tagtctatcc aacacaaaag tttaactgca aagtttttga
    21961 tgatagagga tgtacaattt ggcatacttg tggaaaataa atctaaagta tttttaaatg
    22021 caatcttttt agtccaattt aaggtgtcat tttctatttt acatggaaag tcgatattat
    22081 ataaatccca actgttaaac ttagaactat tataaatatt aaaaatttta agcatcttat
    22141 ctgtttgttc tttgtcaaaa tttattttta gattgtttaa tctaatatag ggaattgtag
    22201 gattattaaa aaaataagta tctatatttt taattttttc tatgtattta tcaattgaat
    22261 catcaacacc tatatatctt gtaaagtaga cttctttaat atatgaagaa cgcttatttt
    22321 tataggtagt atcagatata aattcaatta gaggttcagt agactttttt attgaaatct
    22381 ttaatggttt aatagtacaa atcatatttt ttctcctata ataagatgat ataatctatt
    22441 ataaaacaac ttaaaacaat atagtacaat aattttgtag tttcaaaata aaatatgtac
    22501 ttgtttatat aaatgatata taattacata ttaaacatag cttttatgca tttaataaag
    22561 tatgtaaaat ctatgtaaaa agtaagaaat ataaacattt ttttaattat attttaaaaa
    22621 tatcaagcaa gttttattca caaatgaata aaaaatgtta taatatgatt aaagaaaaag
    22681 ataaataatt agcaagattt gtttagctaa aattaacaag ggagcgataa attatgggta
    22741 taacattatc aaaaggccaa aaggttgatt taactaaagg aaatccaggt cttaaaaata
    22801 ttttagtagg tttaggatgg gatactaaca agtatgatgg tggatttgac tttgatttag
    22861 atacagcagc atttttaaca ggagcaagtg gaagtgttac taatgatgga gattttgtat
    22921 tttacaacaa tttaaaacat acatctggag cagttgagca tctaggagat aacagaactg
    22981 gcgaaggtga tggagatgat gaacaaatag ttgttgattt atctaagatt cctggagaaa
    23041 taagcaagat ttcttttact gttacaattc atgatgcaac agaaagaaga caaaattttg
    23101 gacaagtaag taattcttat ataagaatag tagataaaga cacaaatgaa gagttaataa
    23161 agtatgaatt aggagaagat tttagtatag aaacagctat agtagtagct gaaatatata
    23221 agcacaatgg agaatggaag tttaatgcac taggttctgg ctttgaaggt ggtcttgctg
    23281 cattatgtgg aaactttgga ataaacttat aatttcaact ataacaataa actaaagggg
    23341 gatattttcc atggctatag tattaaaaaa aggacaaaaa atcgatttaa ctaagggaaa
    23401 tccaggtctt aaaaatataa aacttggact aggatgggat acaaattcat ttgatagtgg
    23461 ctatgattat gatttagatg taagtgtttt catggttgga gaatctcaaa gagttgaaaa
    23521 agatgaagat tttatattct acaataactt aaaacatcca tctggagcag ttgaacactt
    23581 aggtgataat agaactggtg aaggtgatgg agatgatgag gaaatattag tgaatttaga
    23641 gacaatgcca aagcatatac aaaagatagc tgtaacagtt acaatatatg aggctgctga
    23701 aagaagacaa aattttggac aagtaagcaa ctcatatata agagtattaa attctgaaaa
    23761 tgatgaagaa atattaagat atgacttagg tgaagaattt agtatagaaa ctgcaattac
    23821 agtatgtgag atatataagt ataatggaga atggaagttt agtgcagttg gagcaggatt
    23881 tgaaggtggc ttagaagcac tttgtagaaa ctatggctta aatgtttaat ttaagcaata
    23941 taatatatta aatataaata acctatacaa ataatgtata ggttatatta tataaaattt
    24001 tgtggaggtt tagtatgtcg attaatttat caaaaggtga taaaatagat ttaaaaaaat
    24061 ctaacccagg actgagtaac atacttgtag gtctaggatg ggaccctgtg caacaatctg
    24121 gcggaggttt ttttaaatcg ttatttggtg gaggacaagc tgatatagat tgtgatgcat
    24181 ctgtatttat gcttaatcaa gaaggtaaat taagtggaat aaaagattta atttattttg
    24241 gaaatctaaa aagtgcctgt aaatcagttt tgcatacagg agacaatcta actggtgagg
    24301 gtacaggtga tgatgaacaa atcctagtta atttggataa agttccatca aatattcata
    24361 aattattgtt tgtagtaaat atatacaatt gtgtagatag aaaacaacac tttggaatga
    24421 tagaaaatgc ctatataaga gttgaagacc aaggtaataa gaaagagata gcaaaataca
    24481 atctatctga taattattct gaaaaaacta ctcttatagt aggagctata tatagaaaag
    24541 atggaagttg gcaatttaaa gcaattggtg aaggaacaaa agatgcgggt cttaaagaag
    24601 taatgcaaaa tttagacagg attgagtgtg catatggaat ttaataatga tatcaaatat
    24661 tataatgtag ataaagaaga agttacaaaa gacttatctg ttaatcctga aaaaggttta
    24721 tcagaatcag aagtcaagac tagaagagaa aaatatggtt tgaatgaatt tactccaaaa
    24781 gaagagggta gtttttggga tgatttaaag gaaagtttaa gtgaaccaat gatagttatc
    24841 ttgattattg cagcagtagt aagtgcagta attggggaaa ctcatgatgc aatagggatt
    24901 gtaggtgcta tagcaatagg tatagcaata ggtatgatta cagaaggtcg ttctaagaaa
    24961 gcagcagagg ctctttctaa gttgactgaa aacatagaag taaaagtatt gagagatggg
    25021 gaagtacatc aaatttctaa gagtgaattg gtaccaggtg atatagtata tattgaaact
    25081 ggtgacatgg tacctgctga tggtagactt atagaaagta taaaccttaa aattagagaa
    25141 gatatgttaa caggagaatc tgatgacgta tctaaaaagt gtgatgtagt agtttctatg
    25201 gaagagatag aatctaaagg agcagtagta gtacaggaac caatacctgc aaaacagata
    25261 aatatggtgt ttggtggtac acttgtagct tatggtagag gagctctagt tgtaacttca
    25321 attggagata atagtgaaat gggtaaaatt gctcaaaact tatcagaaac agatgagcaa
    25381 actccacttc agataaaact tggaaattta ggtggtttga tagccaaagt ttctagtgca
    25441 gtagcaggat tactatttat atttatggtt ttccaaatga taagtaagaa tgtattaaat
    25501 gtagatatgt ctggaatatt accgttttta gaatcaatag acccagttaa aacagcattt
    25561 acagtatgtg ttgcgttgat agttgcagca gtgccagaag gtcttccaac tatgataaat
    25621 atgacacttg caataacaat gcaaaaaatg gctaaaataa atgcattggt taccaaaaaa
    25681 gaagcttgtg aaacgatagg ttctgtttct gtaatatgtt cagataaaac tggtacatta
    25741 actgaaaata gaatgactgt agaagttgca tatgttgatg gcagatatat tgaaagttca
    25801 gaagaggaaa taaatagtta ttttgaagaa aactgtatga taaactctac agctgatgta
    25861 gagcacaatg atggtgatat taagtattta ggaagtgcta ctgaatgtgc attgctttta
    25921 tattacaaaa acgtagatta tagacaagtt agaaaaaatt ctaatatagt agctcaaaat
    25981 ccttttactt cagattcaaa gagaatgact tctgttatag gtcaagataa tcatcatgta
    26041 ctattatcaa aaggagcacc agaagtctta cttgaattat gttcacatat tcaaagagga
    26101 aaagacatag ttccattaac tgaaaatata aaacatgaga ttttagaaga aataaagaaa
    26161 ttgcaaataa aatcaatgag aactttaggg tttgcatata aagaaatctc acaagctgaa
    26221 gaagaagcag cagtaactgc tgaaagtgat gctatgaatg taagtgcaat ggaaaacaat
    26281 ttagtattta gtggttttgt tggtataaga gacccactta gaaaagatgt tatagagtct
    26341 gtaaatacag caaataaagc tggtgtttct gtaaaaatgc ttacaggaga taatataaat
    26401 acggcaagag ctataggtga agagttaggc ctattaaaaa ataatatgag agtggttgaa
    26461 gcttcatata tagatacttt agatgatgaa gaattaaaac aagagataca aagtatttca
    26521 atcgtggcaa gaagtaaacc agatagtaaa atgagaatag tttcagctct gcaaaagagc
    26581 ggagatgttg ttgcagtaac aggtgatggt ataaatgatg ctccagcact ttcaaaagca
    26641 gatgttggta tagcaatggg tatatctggt acagaggtat ctaaaaacgc agcagacata
    26701 attttaactg atgatagttt tagtaccatt gtaaaaggta ttaaatgggg tagaggaatt
    26761 tatgataact tccaaagatt cgttcaattc caattaactg taaatgtaat tgcattttta
    26821 gtggctataa tatctcaagt tatgggacaa gagatgccat ttactacaat acaattatta
    26881 tgggtaaaca tcattatgga tggaccacca gcactagcat taggtttaga gccagttagg
    26941 gaccatgtgt taaacagaaa acctgttgat agaaaagcca atataatttc taagtcaatg
    27001 gtgtatacaa tagtattaaa tgcattttat ataacagcaa tactaatgtt acaatctaca
    27061 tttaatttct taggagttga tgaagcgcat aagggaacag taatgtttgc gttatttgct
    27121 tttagtgcac tgtttaatgc atttaactgt agagagttta atgcagatag tatcttccct
    27181 aattttacac ataacaaatt agcactacaa ataatagtgt tgacaggaat tgcacaagta
    27241 atatttactc agctattcca agatttcttt aactctgtag caatggattt tatgacatgg
    27301 gtaaaagttt tatgtgtagc agcaagtgta attgtggtta atgaagttgt aaaatgtata
    27361 ataagactgg taaaaccaaa aaagactgct taaaacaaat tttttagaag aaaacagact
    27421 gactcaaaat atgaattttg ggtcagttct gaataaacat ttaaattggg aggatagtta
    27481 tgggtgactc aaaggtattt gaaaaaatat tctccagcca atcagataga ggaaattaca
    27541 ctccttcaaa gggatatcta agttatttta tttcgtatat tggtttagaa gatgaagttt
    27601 tgtacaatct cgagatattt aaaacaaaac aaaatattga tagcaaaaaa gatattgctt
    27661 tatttactga tgttatagca aatcctagtg attttgatat tataaattat tttaaaagtg
    27721 gacttcaaaa atatagaact agcatggaag atgtagatat aaatattttg ggatttgaag
    27781 agattgatta taaaataaag caagcaatgg atagagtgtt aaaagaagag gaaaaagaat
    27841 ttactaatga tagagttaaa caaaatttta tagtgaaaat aatggcatgg ataaaaattt
    27901 atatcggtgc tttagatata aataaaaatg aagctccaaa agtaatattt tatggagata
    27961 taaaaaaaca tgaagtttat ttactcttaa tattatactt ggcaggtttt gatgttttat
    28021 atcttaatcc aaattcaaag agtaatatag atattttaaa atcagaaaga tacaatatag
    28081 agtttgaaga agccaacata atagaagaaa aaatttcttt tgaagaaagg gttattttag
    28141 gggaaaaaat agataaaagt tctgtaaaaa aagcttttac tgttggggca gaagcatcaa
    28201 agcgtataag tgaggaattg ttaaatgatg caggatttat aaaaccttgg caactccaag
    28261 atagaaaaat aaaaaatcta cttctaagtt caactgttga tgaaatatca atatactgga
    28321 atcaaccact taaattaaga ccaggattta agtttaatga tgctattgta gaagctccaa
    28381 attttctttc taagattaat ggaatatata acgataagaa tgaatatatt aagtttttag
    28441 atttattaag agattcggaa agttcaacat ttattgaatt taatggagat gtagatagat
    28501 tttcaaaagc ttttacaaga gaagctttta gtttatcctt tttattggat tcaaaaggtg
    28561 caatagataa aaactctgtt ttaaataata aagattattc catatcaaca ttagcattga
    28621 atcaacaaat tatgatttta gagaaagtgg aagagttgtt agaaggaagt atgtttttga
    28681 atggtttaag tggagaagac aagataaaag gtctatttac agtacttcac atggataaga
    28741 aatttgttca tatgatgaac aatttcgatt actcacttat aaacccaaaa ttgattatat
    28801 acatgtacaa gagtattgta tttgataaag aaatagtttt tcttatgctt ttacttagta
    28861 agattggatt tgatattatc atattatgtc caggaggaga aaacaatata gagaatgtta
    28921 taaataacca attgattgat gtacacagat tggataaaat ggtatatgac cttaaattaa
    28981 attcgcttga aaatgatata cctttactta aaaaaatttt tggaaagagg agacgatttt
    29041 agatggggat aaatataaat agtttttcaa aagataagaa ctctgtagag gttactgaga
    29101 atttgcctac agcagttcaa gaagaaaact tcgatataat ggaatataca aataataaaa
    29161 aaaatgaact gagaaagtct aaggaagttg aggcattgac atctcttata gaagtagaaa
    29221 atccagatac gattttacag tttggtagga aagcatctga aggagtagca agagtttctg
    29281 atagcctttt aaatacaata aaattaaata gaaatgaaga aaattctaaa atgctggttc
    29341 atttaactaa aataatggac aagtttgatt tagacgattt tcaagaaact aaggaaccaa
    29401 atttcgttca aaaacttttc aaaaaagcta ataatgccat tgaaatgatg tttcaaaagt
    29461 atgaaacatt gggaggagaa gttgagaaaa ttcaaattga gcttgaacaa tacgaaagag
    29521 atatagcact ttctaataag caaataggtg ctatgttaaa tgaaaacttt gagttttaca
    29581 atgaacttca aaaatatatt gttgctggag aaatggcaat agaagaaatg gataatgaaa
    29641 tactaccttt ctttaaacaa aaatcagaga ctagtggaga ccaaatggat gttgtcaatt
    29701 atcaagaact attaaaagtt tatgatatgt taaatcaaag agtctatgat ttaaggatag
    29761 cagaaaatat agctatccaa acaataccta tgcttagagg tatgcaacat aataattatg
    29821 gtttaattag aaaaataaat agtgcatttg tcgtaacttt accagtattt aaacaatgtt
    29881 tatctcaagc tatattactt aaaaagcaag aattacaagc aaaatctcta aaagcacttg
    29941 atgacaaaac taatgaatta ttgcttagaa atgcacaaaa tgtaagtact caaagtgcac
    30001 aaatagctag aatggctgga acaagttctg ttcaaataga aacattggaa aaaatgtaca
    30061 atactattaa atctggtata gatgaaacta tgagaataga agaaaacaat agagctttaa
    30121 ttaaggataa tacaaagaga ttggaagaat taaatacgac tataatttat aataagtagt
    30181 attatattaa gggctaattt gtataaagtt ggccctttct atgtatatga ggaggctaga
    30241 cattgaaata tttcgattat atctgcaaag atgatttgga gagaattttt ttgaaggagc
    30301 cagaagattt tagtgctaag actgaaaaag atgtgttgaa atatgcactg ggcgcttttt
    30361 tatacgttcc agctactcaa tataatatga tttataagag tattataggg gatgttaagg
    30421 gagttagacc attagctatt tgtttagaag atgctgttgg agtaaatgga gagttagagg
    30481 ctatagaaaa tcttagatta attttaaaaa atattagcaa tgaaagtata actaataaag
    30541 atggaatacc actaattttt gtaagaataa aagatgtgga gcaactgttg agaattaaag
    30601 agattataat taaaaataga cattctataa caggaatatt aattcctaaa gcaaattcgg
    30661 agttaataga aaattgtata gaagctttag attctatgaa tcttcaagat atgtatgtta
    30721 ttccgataat agagacaaaa gaatttatat ataatgaaaa aaaagaactt agttttacta
    30781 atttgtacaa tgctatttta agacataagt caagaatatt aagcattaga gtaggcataa
    30841 ctgatatatt agggatgtat ggtataagga gagataagaa cttctctata tataataatt
    30901 tgatttgttc ttcatttata ttagatataa tcacatatct tcaaagacca gagttagata
    30961 tacctataag tggtggagta tcagaatttt ttgatatgac gaataaggat ataagaaata
    31021 agtatataga agaaatatta ttggataaat atcatggatt aattggaaag accgtaattc
    31081 atccaatgca aattcaaatt gtacaagcgc tatctacagt atcatatgaa gattttactg
    31141 atgctttgga tatactggat agtacagaca gtaagtatgg tgtgagcaag ggtgtattag
    31201 gagagagaat gaatgaaaca aatccacatt ttttatgggc taaaaaaaca ttgattctat
    31261 ctaaaatata tggtgtatta aataaaggag tagactatga agaattgctt aaattttaat
    31321 tgtgagattg acattattga taatccattg aatttagaac taaatgattt tttagacgta
    31381 ggaataagaa ataatagcaa aagacgtttt ttgtttatct caaagaaact cggtaaacat
    31441 ttagcatgta agccaagtga gatggataat ttgggaaaat taatggctac aatttataat
    31501 gaaaaaaata aggaagaatc aaatggtact gttatagctt ttgctgaaac tggtacagct
    31561 gttgggcata gtttttttga ttacttatgt ggagattatg aatttattca tactactaga
    31621 gagcagtttg acgagttaga aaatctggaa tttttagagg aacattccca tgcaacagac
    31681 cagaatttat actttggtat gcttaacaac tttaaatttg gagatgagat aatattggtt
    31741 gatgatgaga ttaccacagc caatacatgc atgaatataa ttaggaaaat tcaaagcatt
    31801 tatcctaaaa agaaatacac tatatgctca atactcaatt gggtaagtga tgaaaattta
    31861 agtaaagtta ctcgtttaga aagagagcta gattgtaaga tagattttgt atacttattt
    31921 aaaggaaact ttaaatttta tatagatgaa gatattattt caaattacaa cgataattat
    31981 agtattacta aattagagac tatttcaagt gataaagtaa atttagtagt aaatcatatt
    32041 tatgttgatt tagaagatta tattggaaat aagaaatatg taaaatatac aggaagattt
    32101 ggaattaata gaagagaaca atttagatta aaagaaatcg ttaagagaga aagtaaaaaa
    32161 ataactatgg ccagtaaaga atctttaaaa aatgaaaaag aaaagattct atttttaggt
    32221 actgaagaat ttatgtatat acctatgctg tttgcaaaag aaaaagaaaa tcaatatgat
    32281 atatactatc attcaacaac aagaagtcca attattaata ttgataagaa taattatcct
    32341 attaaaagta aatttatgct aaagagtttt tataatatag atattcaaaa ttatatttac
    32401 aatatagata aacataattt ttctaagtgt tatttatttg tagagctgaa taaaagtgaa
    32461 aattcttatc tagaatttat tgatataatt aaaaaaacaa gtataaaaga attaactata
    32521 gtatgttgta gctaaagtgt tttaacagga taaaatatat cacgatttta agataggagt
    32581 tgagtcatat gagagatttt agcacgtatt ctaaagatga tgttattttt ttattaaaag
    32641 atatatcaaa tttaattgaa gaagaaggaa ataaagaaag agaattaaaa atacaaaatg
    32701 gtagacatta ttcagaaatg ataccaatag agtatgaagt tagtgaagat tatctgaatt
    32761 tatatcatga gaaactaaga gaaaataaag ataaacttgc attttctata ggagttatgt
    32821 gtgaaaaaat aattaaaaaa aatggaaatg atgtagtact agtttcactt gctagagcag
    32881 gaacacctat tggtattttg gcaaaaagat atataagaag caagtataat gtaagtttac
    32941 ctcactatac aatctctata ataagagata agggaataga tatgaatgca attgaatata
    33001 tagtaaagaa tcatccgagc tcaaaaattc aatttttaga tggatggact ggtaaaggaa
    33061 ctatatcaaa agaacttgaa aaagcatgtg atgaacttaa tgttaaattt aaaaaatatt
    33121 ttgactcaac acttgctgta ttagctgacc ctgctggata ttcaggactt tatggaatta
    33181 gagaagattt cttaatacca agtgcatgtt taaattcaac agtttctggt ttagtaagta
    33241 gaactgtact aagagaggat ttaattagta aaaatgactt tcatggagct aagttttata
    33301 gacatttaaa agataaagat gaatctatga actatatcga aactatagaa gaatgtttta
    33361 aaaatcaatt taagaatata tctgatgaag ttgaaaactg ggaaagtgac attataacaa
    33421 gagatggata ctttgatgtt ttaaatataa aagaaaagta taacataaca gatattaact
    33481 ttatcaaacc aggtgtaggg gaaacgacta gggttttact tagaagagtt ccgtataaga
    33541 ttcttgttaa agacttaaat gataaatctt tagaccatat attcatatta gctaaggaga
    33601 aaaatgttga agttgaacaa atggatttaa aagcctataa atgttgtgga ataataaaaa
    33661 atatgaaaga tatttaggaa atatattaga caggagaata ttgaatgatt gttttttcag
    33721 atttagacag gagtatcatt tactccaata aatttttaaa tgctgatagt aaatatgcta
    33781 atatagaaat ttatagggaa aaagaaatat cttatatatc tttagatact ataaatttaa
    33841 taaagcaaat acaatattat ggtatgttta tacctacaac tactagaaca gtggagcaat
    33901 ttaagcgtat agaatttaat aaatatggga tttattttcc ttggagtata acttcaaatg
    33961 gaggagttat acttaaagat aatgaaatat tgaaatcatg gagtgaaaaa atagataaac
    34021 taaaatctga ttatgagccc attgaaagta tgattcataa atttaaagac tatttgaatg
    34081 tagatggaat aactaatttt aaagttgcag aagatacatt tttttatata gttgttgatt
    34141 tatcaagatt taatttggat tcaatcaaag aatatacaaa tatattggaa tcaaaaaatt
    34201 ggaagtttta tgtgagtggc agaaaaatat attttatacc aaaagaaatt tcaaaagaaa
    34261 atgctataaa atacttgact aaagaattag gaatagaata tttttatgca gttggagatt
    34321 ctattatgga ctatggtatg cttaatatct caaataaatc ctatgtattg aagcatggtg
    34381 atataaataa aaatgaaata gaaaattcat ttatatcaag ttctttcaat ggaatgagtg
    34441 gaactgaaga gatactatct aacattttag acgagaactg cctacttaat atataatgac
    34501 tgatatataa ataataaact agaaaaatct gaaataaatt atagtaaagt ttatcatact
    34561 aatttatttc agatttttat caatttatat ttaagtaaga actcaatatg ttaaaatgaa
    34621 ggtggagtca ttgcccagac tgagatacat acctcatcat cataattttc atacacatgt
    34681 ggtatagtac tgttaaaata aaaactatct ccttcctcca atagatattc cttatttcca
    34741 agtgttatct tcattttacc ttttattata tagccacatt cttctccttc gtgtgtcaca
    34801 aattcactgt ttttgtgtgt atgaccatct agtgtaacaa gtataaattc tatttttttc
    34861 ccagataaat taggaacaag aagttcgtaa aattttgtag aatcattagt tactattttt
    34921 tttcgattat ttttctttac tactatatca gagtcttctc ttccaatctc ttcaaaaaaa
    34981 taacctatat ttatatcaag agcttttgat attttccaca ttgctacaat agatggaaca
    35041 accatattac gctcaatttg acttattaag cctgtactta aatctgaatt ttttgctaaa
    35101 gtagagatac ttatattttt agattttcta agagatctaa ttttgcttcc aacatctata
    35161 ttcattagat acaaaccccc tcagacatga ttataacata taatttataa agattaataa
    35221 aataaaaaaa aagttttaca atacaagtat tttttatgct atattgaatt ttctgaaaag
    35281 atgcattata atagtattaa tattcaatat attgaatatt gataacgata ttttaattta
    35341 atgaaatgga ggtaattaag atgaagagtt ttgtaagtcc tgataatatt tgggtattat
    35401 gggcagttgt aactggatgg gcagcattta gtatttattt ggaacaaaag tacaattggg
    35461 catcaaaagt gtcgggagca ataatagctc ttgtaggagc aatgttatta tcaaatctaa
    35521 atattatacc agtagagtct gttgtatatg accaagtatg gggatatgtt gttcctttag
    35581 ccatagcttt attgttatat caatgtaaca ttaaaaagat atggaaagaa agtggaagat
    35641 tgctcattat atttcttgta ggttctgtag gaactgtact tggagctatg attggatttt
    35701 tagcattaaa aaatgtagta ccagatttaa atattgttgc agctatgatg acaggttctt
    35761 atataggtgg aaatgtaaac tttgctgcta tgtcaggggc gtttgatgcg ccaggagaat
    35821 tagtatcagc aacagttgta gcagataatc ttttaatggc attatatttc tttgtcttaa
    35881 tagctatacc atcaattgga ttttttagaa aacattttaa acatccacat gtagatgaaa
    35941 tggaaagtat aggtataact gaaggagaaa cagtagcagc aaattattgg ggaagaaaag
    36001 aaatatcttt aaaagatata gcaatagcca ttggtagcgc ttttataatt gtcgcagtat
    36061 ctgtagaact atcaacttgg tttaaagcta taataccatt atcaaatcct tttttagcaa
    36121 tgttaaatac tttatttgga aatcaatatc taataataac tactttgact atgttgtttg
    36181 ccacatttat gcctaatttc tttggtgata taaaaggagc acaggaattg ggtacatttt
    36241 taatatatat attctttgtt gtagtaggtg ttcctgcttc aataacatta ataattcaaa
    36301 aatcaccatt actactttta tactgcggaa taatggtact aataaacatg ctagttacat
    36361 ttatagttgc aaaaatattt aagtttagtt tagaagaagc tattcttgca tctaatgcaa
    36421 atattggtgg tcctacaaca gcagcagcta tggcaatatc aaaaggatgg tctaagctag
    36481 tagggccaat acttatagtt ggaacatttg gatacatagt cggtaattat tttggtcttt
    36541 tagtaggaaa tattttgata taattttacg tgaaaaaaag ttgtttttta gaaagatttt
    36601 tatttaattg gaggttgtta tgattttcga tggacattca gatacttggg cagatgtaac
    36661 aataagaagg cttaacgggg aaaaagatgt atttagaaga atccatcttg ataattttaa
    36721 aagagcaaat atagaaggag ctatttttgt aatatggata gacccaccat atgataaaga
    36781 gcctgaaaaa agagttaaac aaattataag ctctataaaa gatgaaattg aagatatgaa
    36841 agatgttgta aacatagtaa aaaaatattc agatatagat aaaggtataa gtgaaaacaa
    36901 aataaatatt ttaattggtc ttgaaggatt aagccatatt ggaagaaata tagatttact
    36961 agattatttt tataaagaag ttggtgcaag acatgcatca ttaacttgga atgaacagaa
    37021 tgaacttgct acaggatgtt taggggatga aaatcgtggt ttaactgaaa taggtaaaaa
    37081 agcagtcaat aaaatggaaa aattgggcat gatagtagat gtgtcacatt taaatgaaaa
    37141 atcattttgg gatttaatgt ctgttgcaag taagccagtt atagcttctc attctaatgc
    37201 taggagttta tgtgatgcta aaagaaatct tacagataat caattaaaag aaatttcaaa
    37261 tacaggtgga ttagttggaa taaatagttt tagaggattt actaatagta atcctaagaa
    37321 gcaagattta atacatttgg ttgaccatat agactatatg gtaaatctta taggtataga
    37381 acatgtatct tttggttttg attttagtga ttacttaggg aaagagacta taggttcttt
    37441 tactggaaat gctagtgtgc caagtgtaga tggtcttgaa agcacacata cagcacagaa
    37501 catagtagta gagcttgaaa atagaggtta taaaaagaaa gatatagagt taatctctag
    37561 tggtaatttt tataggctct ttaaagatat actaaaataa tataataaag aacttaaatt
    37621 tataaaatag tatatttata caaataatat ctacatattt aaattggcgt tcataattta
    37681 ttaaattaaa ttttaatgtt actagaagtt tcaattctag taacattttt tatgttaaaa
    37741 acagatatag atatatattg acaatgataa ttattattga taaactataa aagtgaaaat
    37801 gataataatt ttcattttaa ttaataaaag atttttaagg agggagaatg aagaaaaggt
    37861 atttagtcat aggaataata attttgtctt atttttcttt atttatagga gctgaagata
    37921 taaatataat gcatatattt gccaaaaatc agcataaact tatgatattt atcatgagta
    37981 gaataccaag attaataagt atattgattg ctggtgtagg aatgagcata gcaggtctta
    38041 taatgcaaca gataagtaag aataaatttg tatcacctac aacaggtgct acaatagatg
    38101 cagcacaatt tgggatagta atatgtatgc ttttagtacc aactgcctct atttttacaa
    38161 agacaataat agcatttgta ttttctcttg tagggacttt tacgtttatg aaaataatag
    38221 ggaagttaca atttaaaaat ataatctttg taccattagt aggtataatg tttggaaata
    38281 taataggttc tatgacggat tttatcgcgt ataaatacga tttgagtcaa aatgttagtt
    38341 cttggatgca aggtgacttt tctatgatac ttaaaggaaa ttatgagata ttatatatca
    38401 caatacccct tataatatta gcatatattt atgcaaataa atttactgtt gttggtatgg
    38461 gtatggattt tgcgacaaac ttaggtctta gttacaaaag aattgtcaat ataggtctga
    38521 taatagtagc acttgttaca gtatgtgtag tcgttacagc tggtaatatc ccttttatag
    38581 gtctaatagt tcccaatatc gtatccttgt atatgggtga taatataaga gcaagcatat
    38641 ggtatactgg tttacttgga gcgatatttg tacttatatg tgatattttt ggaagaatta
    38701 ttatttatcc atatgaaata tctatagggc ttacagttgg agttatagga agtatactat
    38761 tcctttattt aatattaagg aggaatgtaa atgaagctta gtctcaatca aaaaatttac
    38821 tttgtaagta tattactatt catatcaata gcacttttta taatttatgg aatagacatg
    38881 aatcatttag aatatgcatt atcacaaaga ataccaaaaa tatttgctat ggtattaggt
    38941 ggaggatgta tagcatttac tactgtagtg tttcaaacaa ttacaaacaa tcaaatatta
    39001 acaccaagtg tactaggttt agattcattg tatgtaatga ttcaaactat aatagtattc
    39061 atatttggtt caagcagtag tctgattata aatgaaaatt ataattttat tattaatgta
    39121 gctatgatga ttggagcttc attgttatta tataagacgt tatttgaaaa aaataaaaat
    39181 aatattttct ttttaatcct agtaggaatg atatttggaa ccttatttaa aagtgcaaca
    39241 acatttattc aggtaatgat agaccctaat gaatttttag ctttacaaac tagtataatg
    39301 gcaagtttaa acaatataaa cactaatgta ttgttaatag catttataat aattattgcc
    39361 ataatacctt ttatatatga tgaaataaag tatttagatg tattgtcgtt aggtaaagaa
    39421 caagcaatta atttaggtgt agattttgat aaagcagtta aaaaaatgat aatattaata
    39481 gcaattttag tctcaatatc aacagcactt attggaccta tgacattttt aggactttta
    39541 cttgctaata taacaagaga aatttttaaa acatataagc atacatactt gatttcgggt
    39601 tctatgttga tagggatgat tacattgata ataggtcaat tttttataca acatgtattt
    39661 aagtttgata caactttaag tgtagtaata aattttatag gaggaattta ctttattcaa
    39721 ttactattaa agggggcaaa tagatgatag agataaaaaa tatatttaaa agatataaga
    39781 ataaaaatgt tgtagatgat gtctctttta gtatagaaaa gggaaagata acatctttta
    39841 taggaccaaa tggagctgga aaaagtacag tactatcaat agttacaaga cttataggag
    39901 gtgatggtgg agaagtaata atagaaggaa aaagcttgac taattatagc aataaagaat
    39961 tagcaaaaaa aatagctatt cttaaacaat ctaacaatat aactttaaag cttacaataa
    40021 gagaattagt tggatttgga agatttccat atagtgaagg taatctaact aaagaggatg
    40081 aaaattatat tgatgaagca atagaatata tgaagttgac tgatatacaa cataagtatt
    40141 tagatgaatt gagtggaggt caaagacaaa gagcttatat agcaatggta atagctcaag
    40201 atacagagta catattactt gatgaaccac tgaacaattt ggatatgaac cattccgtac
    40261 agatgatgaa ggtacttaga agtctttgtg atgagttaga taagactata gtattagtaa
    40321 tgcatgatat aaattttgca tcatgttatt cagataatat agttgcatta aaaaatggaa
    40381 aagttaaaaa agtaggtcgt acagatgaga tagtaaatga aaaagtttta gaagatattt
    40441 atgagatgaa ttttaacatt aagaatataa atggaaatcg aatctgtatt tatttttaaa
    40501 atattgtata tgaaaataaa cttgctatat gattttttat aaagttttta aattaaaata
    40561 aagattaata ggagagagga aaatattgat atgaataaaa aagcagcaat agtagcagca
    40621 gtagctatta taggattagt gacagtgttt gcattaggtg gaagtaaaaa gaatgaatca
    40681 aaaacatcag aaaattcaaa taatactatg aagataactc ataacttggg agaaacagat
    40741 gtaaaattaa atcctaaaaa agtagtggta tttgattatt cggctttaga tacaatggat
    40801 gcattaggtg tagctgagaa tttagtagga cttccaaaag cgagtttacc agcatcctta
    40861 gaaaaatata aggatgacaa gtatgcagat ttaggaggat taaaagaacc agatttagag
    40921 ggtataaagt cggcaaatcc agatttaatt ataataaatg gaagacaaga agatttttat
    40981 gagcaattgt caaaaatagc accaacaata agtacaagta aagatgataa gaagtactta
    41041 gaatctgtta aaaataatat agacaaaata gcaaaaatat ttggagtaga agaaaaagca
    41101 aatcaagaat ttagtaaaat tgaaaagaaa atagaaactt tgaataaaaa agtaacagat
    41161 aaaaatttaa atgctttaac tataatggta aatgaaggta atttaagtgt atttggtgaa
    41221 gaatcaagat ttagtatatt atacaatagt tttggatttg aaaataaaga taaaaatata
    41281 aaagaatcaa gtcatggaca aaatattact tttgaatata tagctaaaca aaatccagaa
    41341 gttatgttcg ttatagatag aggaatagct actggcagtg atgtaaaaga aagctcaact
    41401 gcaaaatctg ttttgaacaa tgatataata aaatctatgg atgcatataa gaatgataat
    41461 ataatatatt tagattcacc aacatggtat gtaaatgatg gtggtctaac atctttaaat
    41521 aaaatgatag atgatgcttc aaaagcggta aattaataat ccgatataat caaaatatat
    41581 tttttataaa atgattatca aataaactag gagtttttaa gaactcctag tttatttttt
    41641 gcataaaaaa gatatagttt aaacaataat a
//
LOCUS       DBGJFU010000022        37924 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.022, whole genome shotgun sequence.
ACCESSION   DBGJFU010000022 DBGJFU010000000
VERSION     DBGJFU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.022"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            148..1365
                     /gene="ilvA"
                     /locus_tag="ACOIAS_001122"
     CDS             148..1365
                     /gene="ilvA"
                     /locus_tag="ACOIAS_001122"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HGZ2700841.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1460..1840
                     /locus_tag="ACOIAS_001123"
     CDS             1460..1840
                     /locus_tag="ACOIAS_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGZ2700842.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2124..2606
                     /locus_tag="ACOIAS_001124"
     CDS             2124..2606
                     /locus_tag="ACOIAS_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2700843.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2627..3442
                     /locus_tag="ACOIAS_001125"
     CDS             2627..3442
                     /locus_tag="ACOIAS_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2700844.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3495..5045
                     /locus_tag="ACOIAS_001126"
     CDS             3495..5045
                     /locus_tag="ACOIAS_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2700845.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5142..6494
                     /locus_tag="ACOIAS_001127"
     CDS             5142..6494
                     /locus_tag="ACOIAS_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGZ2700846.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6625..7383
                     /locus_tag="ACOIAS_001128"
     CDS             6625..7383
                     /locus_tag="ACOIAS_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700847.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLISNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELFNLLHTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7430..8149
                     /locus_tag="ACOIAS_001129"
     CDS             7430..8149
                     /locus_tag="ACOIAS_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HGZ2700848.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8273..9673)
                     /locus_tag="ACOIAS_001130"
     CDS             complement(8273..9673)
                     /locus_tag="ACOIAS_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HGZ2700849.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9677..10567)
                     /locus_tag="ACOIAS_001131"
     CDS             complement(9677..10567)
                     /locus_tag="ACOIAS_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2700850.1"
                     /translation="MTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKMIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10846..11493
                     /locus_tag="ACOIAS_001132"
     CDS             10846..11493
                     /locus_tag="ACOIAS_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2700851.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11817..14456
                     /locus_tag="ACOIAS_001133"
     CDS             11817..14456
                     /locus_tag="ACOIAS_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGZ2700852.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14514..15617)
                     /locus_tag="ACOIAS_001134"
     CDS             complement(14514..15617)
                     /locus_tag="ACOIAS_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2700853.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIHLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            15978..16799
                     /locus_tag="ACOIAS_001135"
     CDS             15978..16799
                     /locus_tag="ACOIAS_001135"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGZ2700854.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16878..17089
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17176..18492
                     /gene="argH"
                     /locus_tag="ACOIAS_001136"
     CDS             17176..18492
                     /gene="argH"
                     /locus_tag="ACOIAS_001136"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGZ2700855.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18741..19079
                     /locus_tag="ACOIAS_001137"
     CDS             18741..19079
                     /locus_tag="ACOIAS_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HGZ2700856.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19206..20495)
                     /locus_tag="ACOIAS_001138"
     CDS             complement(19206..20495)
                     /locus_tag="ACOIAS_001138"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2700857.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20575..21279
                     /locus_tag="ACOIAS_001139"
     CDS             20575..21279
                     /locus_tag="ACOIAS_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGZ2700858.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21371..22417
                     /gene="selD"
                     /locus_tag="ACOIAS_001140"
     CDS             21371..22417
                     /gene="selD"
                     /locus_tag="ACOIAS_001140"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:21419..21421,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGZ2700859.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22468..23847
                     /gene="selA"
                     /locus_tag="ACOIAS_001141"
     CDS             22468..23847
                     /gene="selA"
                     /locus_tag="ACOIAS_001141"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGZ2700860.1"
                     /translation="MLPSVDEVLSDNRIVEIINEYPRSLVLESVREVIDLKRQFILRL
                     KEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLGRSLLSEDIKDE
                     LWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNAAAVLLVLSTMA
                     KGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYKEAITEDTNVLM
                     KVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKYGLSYEPTVLDS
                     IRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDKLTICALEATLR
                     MYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINIEDGFSQVGGGS
                     MPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDVRTIFDDEFDVI
                     VEELRKAFN"
     gene            23961..25844
                     /gene="selB"
                     /locus_tag="ACOIAS_001142"
     CDS             23961..25844
                     /gene="selB"
                     /locus_tag="ACOIAS_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGZ2700861.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            25949..28687
                     /locus_tag="ACOIAS_001143"
     CDS             25949..28687
                     /locus_tag="ACOIAS_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HGZ2700862.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28806..29750)
                     /gene="manA"
                     /locus_tag="ACOIAS_001144"
     CDS             complement(28806..29750)
                     /gene="manA"
                     /locus_tag="ACOIAS_001144"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HGZ2700863.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29923..31284
                     /locus_tag="ACOIAS_001145"
     CDS             29923..31284
                     /locus_tag="ACOIAS_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2700864.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31372..33261
                     /locus_tag="ACOIAS_001146"
     CDS             31372..33261
                     /locus_tag="ACOIAS_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2700865.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33276..33731
                     /locus_tag="ACOIAS_001147"
     CDS             33276..33731
                     /locus_tag="ACOIAS_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2700866.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33790..34107
                     /locus_tag="ACOIAS_001148"
     CDS             33790..34107
                     /locus_tag="ACOIAS_001148"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGZ2700867.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34132..35394
                     /locus_tag="ACOIAS_001149"
     CDS             34132..35394
                     /locus_tag="ACOIAS_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGZ2700868.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35499..36563
                     /locus_tag="ACOIAS_001150"
     CDS             35499..36563
                     /locus_tag="ACOIAS_001150"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HGZ2700869.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36617..37795
                     /locus_tag="ACOIAS_001151"
     CDS             36617..37795
                     /locus_tag="ACOIAS_001151"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2700870.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT        14894 a         4154 c         6652 g        12224 t
ORIGIN      
        1 tattgtatgc aattgttgta tttttaggtt ttttgtgatt aattaaaaaa ttataaaaat
       61 cgtttacatg ttgattttta aaaaaaaaag atatagaatt atatagaaag taataaacaa
      121 aaattggatt tacgggagtg gaatgaaatg atgaatgtta ccttaacaga tgtaaaagag
      181 gctagagaga ccattaagaa catagtaaaa agaactgact tacttgaaag tgttaaactt
      241 agcgaaaaaa caggagcaaa tgtgttctat aaatgtgaga atcttcaaaa aacaggttct
      301 tttaaattaa gaggagcttg taacaaaata gcgagtttaa cagatgaaga aaaggcaaat
      361 ggagttatag cttcaagtgc agggaatcat gctcaaggag ttgcattggg agcaaaaatg
      421 actggtataa aatctacaat agtaatgcca gcaactgcgc ctttagcaaa agttagtgca
      481 actaaaggtt atggagcaga agtagtttta aatggtgcgg tttatgatga tgcatatgca
      541 aaagctgttg aaatacaaaa agaaacagga gcaactttct tacatccatt caatgataag
      601 tatgttatag caggacaagg aactatatct cttgaaattt tcgaacaatt agacaataaa
      661 gttgatacta tactatgccc agttggtgga ggaggaatca tctctggtgt tgctgttgca
      721 gcaaaagcat taaatcctaa tgtaaaaata gtcggtgttc aaactgcaaa tataccttca
      781 atgaaagagt caataaaaaa tggaaaagta acaacagcat ttaatgatac tacaatagct
      841 gatggtatag ctgttaaaac tccaggagat ttaacttttg aaataataaa tgaattggtt
      901 gatgaaatag tagtggttga agagacagaa attgcagaat caatattatt tatgatggaa
      961 agtcaaaaaa tagtttcaga aggtgctggt gctgtatgta cagctgctat attaagtggt
     1021 aagtatgtac cagcaaaaga tgaaaatgta gtttgtataa tatctggtgg taatatagac
     1081 ataaatacgt tatatagaat aataggtgtt gctttagcta aagaaggaag aagatattca
     1141 tttagtacaa ttatggaaga taagccagga aactttgctg agttaactag aataataagt
     1201 gagaatggtg gaaacatttt aagtgctaat caaggtaaat tatcagcagg ggaagctcta
     1261 ggaaaacaaa gtgcggaatt tatattagag acaatagatt atgaccatat agctagaata
     1321 aagaaagcta tagaagaaaa aggttttaaa attatagaat tataataaaa agtgtatata
     1381 atatacaaga aaaatagtat aatagtgtat gaagatacat tttatgtgta ttagattaac
     1441 gaattaggag gattttaaaa tgaaacatga agtaatacat actaatgatg caccagcagc
     1501 tttaggacca tattctcaag ctataaaagc tggaaacttg ttatttgtat ctggacaagt
     1561 tcctcttgta ccagagacta tggaagtagt tgaaggagat gtacaagctc aaactgctca
     1621 atctttaaaa aatcttaaag ctatattagc tgaaagtgga gctgattttt caaatgtagt
     1681 taagactaca gtatttataa aagatatgaa tgaatttggg gctataaacg aagtttatgc
     1741 tgaatatttt ggagaaaata aaccagcaag agcttgtgta gaagttgcta gattaccaaa
     1801 agacgttaaa gttgaaatag aagtaatagc tgtattataa aaatacaata tttttaattt
     1861 gaaaaggctg aaaaactata ttctagtgat tcagcctttt tataaaaaaa taaatcacaa
     1921 taaagaacat gataaatatt gtcgaattaa aaaataaaat taaaataaat attgcaatat
     1981 ttttgagaag gttttataat gagtatataa tttgaaacgt gtaactatta aattaggcat
     2041 gagttcttgg atatttttat cctgaggatt catgcctttt ttgtattgaa attcaaaaat
     2101 ctatttaata aggaggaagt actgtgttaa attttttcaa gaaaaataaa agctataagt
     2161 tacatgcagt agtgagtggt aacagcatta atattgagaa agtaaacgat tctgtgtttt
     2221 caaaaaagtt aatgggagat ggagttgcaa ttattcctaa tagtaatgtt gttgtagccc
     2281 cttgtaatgg taaagtaact gtgcttacag aatcaaagca tgcctttggt atggtctcag
     2341 atgagggtgt tgaaatatta gtccacatag gaattgatac agttagttta cagggtgagg
     2401 ggtttaaaaa tgaagtgtct caaggagata ctgttaaaaa aggtagtcca ataataagtt
     2461 ttgaaagaga aaaaataaat tctcagggaa ttgattgtac aacaataata attgtattaa
     2521 atcatagcga attttcagaa ataaattgca tggtagaaaa tgaagttgtt gcagggcaag
     2581 atactgttat tgaaattatg aaataataga aagggattaa ttcaatatga gagtagtaaa
     2641 aatctttaat aataatgcat tatctacagt aactaatgat aggaaagaag ctattttact
     2701 aggtcttggt ataggtttta ataaacgtcc aggagataaa gttaatgaag ataaaataga
     2761 aaagatttat tatgttcaag accacatgca aacaaaattt ttagaattat taaaaaatgt
     2821 tagtccagaa gtgatggatg cttcacaaca aattataagt cttataccag gaaatgaggg
     2881 aaaatttaat aataaaggga tattatcttt agtggaacat attagttttg caatagatag
     2941 aatgaaaaat aatgtttttt tacctaattt aatgcttttg gatataaaga tgatgtattc
     3001 aaaagagttt gaattaggag taaaagcttt agaaattata tatagagtat gtcatatcca
     3061 tttgcctgaa gatgaagcag gttacatagc tttacatttt gtcaatttac aatcaaatga
     3121 taatttggca tatgatactt taaaatttgt aaaaggtagt attgatttga ttaaagaatg
     3181 ttatggatta gaattagacg aatcgagttt aagtacatta cgttttagaa ctcatcttaa
     3241 atttcttgct caaagaattt ttaagaatga aatttgtcag gatgataaaa tgattgaaat
     3301 gtatgattat ttaattaaca atcatcctaa aaataaagaa tatttagaaa agttaaatgt
     3361 atatattgaa aaggagttta ggtataaatt agataaacct gaaaaaattt atctactact
     3421 acacctaaca aaaatcttat aagaatgaga tgaattagta atagaaatat atataatttt
     3481 agggggggat aagattgaaa gaaaaactac ttggaggact ggaaacattt gcaaaggcaa
     3541 tggttcagcc attaatgtat ttatcgagtg caggaatatt aatggtggta ggtgtactac
     3601 ttacaaatga tatgttatta ggagtacttc cattttttaa atgggagcca attcaaatat
     3661 ttggaagcct aatttataac tgtataatgg ctattattaa taatcttgct gttatatttg
     3721 ctataggtat tcctgctgca ctagcaaaaa aagagaagca caaagcagcg ttaataggat
     3781 ttatgagtta tataatatac cttacagctt ccaacactat gttaacatca ctaggtcaac
     3841 tagcaaaaga aagcaagatg cttggactga tagggactgg acaagctact attttaggta
     3901 ttcaagttgt agatacaagt gtatttggtg gaattatttt agggtatata gctggatatg
     3961 tatttaatcg tacaagcaac aaaacattta aaggagcttt acagatatac tcgggagcaa
     4021 attttagttt tatatgcatg ttctttgtat ccattttaat tggagttgtg agtaacttta
     4081 tatggccatt agctcaacaa ggaatagctt ctcttgcaaa tgtcatcaaa gatacaggta
     4141 catttggttt attcttatat ggttttttag agagaatatt agtaccaact ggacttcatc
     4201 atttaattta tacaccattt cagttttctg atttaggagg agtgcttaaa gttggtgcag
     4261 atacagtttc aggagcctat gctatagtta tgacagaaat gaatttacca gttgagcgtt
     4321 tctcagatag tatatactac atggcaactg gatttacaaa gatgtttggt tatataggta
     4381 taggagctgc ctttatttat actgcatata agcaaaataa gaagaaggtt aaggcaacga
     4441 ttattccact aataattacg gcagtattag cctctgtaac agaaccatta gactttatgt
     4501 ttgtattctg tgcaccactt ttattctttg tacatgcagt tatagctggt ctttttatag
     4561 ctttattaaa aatatttgat gttactgcat tatgtggagg gaacttatta gtatcagtta
     4621 taatgaatac tacagtaggc gtagaaaaaa cacactggcc agtaatgatg atattaggac
     4681 ttgcacaaat tattgtatat tttgtagtat ttagtttttt aattaagaaa tttaattata
     4741 aaactccagg tcgtgaagat gagagtacag ctacaggcga agaacaatca aaacaactta
     4801 atttagatgc agggatagaa aatataattg atgggttagg cggaaaagaa aatattaata
     4861 cagtagaaaa ttgcatcact agattacgtg ttaatgtaaa agatgaaagt aagattaatg
     4921 aagatataat taatttaacg ccaaatagcg ggattgtaag gaaaggaaaa gatatacaaa
     4981 taatttttgg acttcatgtg catgaggtaa gacgtgcagt agaagatttt ttagaagctt
     5041 attaaagata tatatttata tagcaagggt aaataacagc tttaagtgct gtttactatt
     5101 tattaaagat acatataatt tatagaggag gaataattat tatgattatt actattgttg
     5161 gtggaggaag cacatttact ccaggtattg ttaagtcaat tgcattaaga gaaaaagagt
     5221 tagagcttga ggaaattcgt ttatttgata ttgataaaaa acgtcaagat aaagtagctg
     5281 tagttgtcaa ctggatacta aaagaagatg ttaaaacaga tgttaaacta actgtaacta
     5341 attgtgaaaa agaagcgtat acagatgcta catttatatt tgcacaaatg cgtgttggta
     5401 aatatgaaat gcgtgagcag gatgaaaaaa ttccattgaa atatggttgt gtaggtcaag
     5461 aaacttgtgg ttgtggaggg atggcatacg gactgcgtac aatacaacca atgataaagg
     5521 ttattgatga tgtagagaaa tatgctaaaa aagattattg gatattaaat tactctaatc
     5581 ctgctgcaat cgtttctgaa gcttgtcgta agttaagacc aaatgcaagg attataaata
     5641 tctgtgatat gccaatagct attatagata tgatagcggg gtcattaaat atcaatgacg
     5701 ttcataatat acgctatgat tattttggtc taaatcattt tggctggttt acttcaatag
     5761 attataaaca tagagactta atggaagaga ttaaggaata cataaaagaa aataaaatat
     5821 tattaccaaa atcttatata gaaaacttaa ataaaagtcg tagtacagga aatcgccatg
     5881 ctgtaagtag ctggatacat gtttgggaat ctgtttatac aatgttagaa agcttcccaa
     5941 aatatctgcc aaacacttat ttaacatact atcttttaag taaagaaaca gtggaacatt
     6001 caaacataaa tcgtactcgt gcaaatgagg ttatggaaag ccgtgaaaaa gacttatttg
     6061 aaggtgtgag acattactta gaaactggag aaatttctga aaaagcattt tatgcaggtt
     6121 cacatggaga ttggattagt gatttggcag tttcaattaa aaatgataca cgtagtcgtt
     6181 ttttagttat aactgaaaat cgtggagcta taccaaacat gccatatgat gcaatggtag
     6241 aaattccagc atatattggt aaagatggtc cagaagttat tgctcgaggt ccaatacctc
     6301 tatttcaaca aggattaatg atgcaacaac ttaacagtga aaaattgata gtagaagctt
     6361 atatagaaaa tagttatgaa aaagctttac aagcattcac attaaataaa actattccaa
     6421 gtatgaatgt tgctaaagaa attttggatg aaatgataga agttaataag gaatactggc
     6481 cagaattaag atagtaggat aaaagaaagg gtttttattt aatcattaga tatgttttca
     6541 gagtctttta tcaaaatgta ttcacaaaca tatggcaata tattaaaata aagagtgata
     6601 tttttagaag aatggggtga tgcaatgaac attataaata aaattttttg gcaagaagat
     6661 aggattggca tgattacaaa taatttagag gcagatacac atagccactg tatgttacaa
     6721 ctgttcttag ggatagaaga cagcattgaa ataacagtta atgaaaaatt agttaaatgt
     6781 aattgtatca tagtagataa aaatatctct cattcatttt cagcaagaaa aaaagtttat
     6841 tactctgcta ttattgaacc tacatctata tatgcagaac agcttacctc aaaaatgaat
     6901 gattttggct actggatatg tgacaatgat ggattagaga aattacgtca acaaggaact
     6961 tttttgatat ctaactctag taaagaacaa tatttaagat ttatggaaat gcttaataac
     7021 tatttgaata tacctataac tctcaaacat tatgatgaca gaattactga attgttcaat
     7081 ttattacata cttgtaattg tgataatcat actatttcaa gttttgcaga taaagtgtct
     7141 ctatcagcaa gtagattatc acatctgttt aaagaacaaa tagggatacc tttaaaaagc
     7201 tatatcttat ttcatcaaat ggagtgtgct tttagagaat tactttctgg aaaaaatgtt
     7261 accgaagctt ctatgactgc tggattcgat actccatcac actttgcagg gacagttaag
     7321 cgaatgatgg gaatgcctgt ttctttatct ttgaaagata gcgagttttt gaaagtatat
     7381 taaatattct cgttatataa tgaagttgta ctaagaaagg ggctttgtta tgaataaaga
     7441 atatttagaa gaaacaaaaa tgttaaatta taatgaacca caacttaagc ttttggtatc
     7501 atcaaagaat tggttggaat tagatgattt tcataaaata aaatctattt atgagtttgt
     7561 acagaatgat attttatttg gatataatgc ttctgatatg ttaagcgcaa cacaggtatt
     7621 aaatgatggt atgggacaat gcaatacaaa agctactctt ttaatggcat tattacgtgc
     7681 agtgaatatc ccttgtaggc ttcatgcttt tgatgtaaca aaagattttc aacgaggagc
     7741 tacatccaaa ttgatttcac tattagcacc aaaatatatt cttcatactt gggtagaggt
     7801 attttatcaa gataggtgga ttgctctgga gggtgtcata actgataaaa aatatttaga
     7861 agcaattcag aagaaatttt ttaatcatgg tggaacgttt aaaaagtatg caatagctac
     7921 aaatgatttg aaaaatacat ctattgattg ggatggtaaa gatactttta tccaaaaaga
     7981 agctatcgtg tatgattatg gtatttttcc atcaccagac gtattttttt ctactcattc
     8041 acaacatatg tcaaaattaa aaaatttcat atatgtacat cttatcagaa aaataatgac
     8101 aaaaaatgta tgtaaggcta gaaacaatta tattgataaa aatgaatgag tgtttaagag
     8161 gtaactatat aggaaatata tagaataatt aaaactgtac tggttaaaat gaataaaaag
     8221 gagttatcac agagcaaact taaattgttc ataggataac tccttttctg tattaacttc
     8281 taataatttg atgtcttttt ataaaacata tagatacaat taaaccagta aatgcaagag
     8341 tagctgctat tgaaaatgca acatctattc catgaatgtt agacagcatg tttgaactca
     8401 tatgagcttt cttagtagca cttgtcatta tagtaataag tatagcagaa cctatagctc
     8461 cagatatctg acggagtgtg ttattaattg ctgtggcatg agatataagt tctctatcta
     8521 atgttttaat tccccatgta gttaatggca ttaaaaccat tgaaatacca atcatacgaa
     8581 tacaatacat caatgacaca tgaattaaag atgtatctct gcctaaaaaa gcgaaggata
     8641 aagttcctaa aaacaaacat cctgttccta atattgatag agcatgaggt ccatatttat
     8701 ctaaatgacg tcctgcaatt ggattgagta caaccatcaa tatagcacca ggtaacatta
     8761 atgaacctga agataatgca gaaaatcctc ttacagattg aacgtacaga ggaatcatta
     8821 atgtagcaga catcattgag gcatatacaa tcataattaa tattgtagaa ataagaaatg
     8881 gtttattttt aaaaaccctc aattctaaaa aaggttcttt agattttaat tgacgcaatg
     8941 taaataaaat taatgacata attcctatta atattggtaa atatgtggca atattagtcc
     9001 acccataatt accttgatta gtaaatccaa ttaataaacc accaaaacct agagatgata
     9061 gtactattga aggtatatct aattttagtt tttgagtttc cccaacattt cttaataaaa
     9121 taaatgatag tataacatct aatatagcaa taggggaaag aaacagaaat aaactatgcc
     9181 aaccaaatga atctactaac catcctgata aagttggacc tattgctgga gcaaatccta
     9241 ctgtaatacc aactaaactc atagctgcac cacgtttttc ctcagggcat aaatagagta
     9301 taataacttg taaaagtggc attagagaac cagaacccat tgcctgcaac acacgagcaa
     9361 tcaacatagt tgaaaaatta ttagaaaaaa gtgcaataat acatcctata gaaaagatag
     9421 acattgatgt aataaataac tttctagtac taaatcgatt tattaaataa gctgttgtgg
     9481 gtacaataat acctgcaata agtagataaa ttgttgttaa ccactgacca acatcagcag
     9541 taattttaaa atctgacatt atattaggta atgctgttga caatagtgtt tgactgagag
     9601 aacttataaa agcaccagtc attacaattg ctattattaa atttcctttt ttattgttac
     9661 tttgggtttc cataatttat aacgtctcct tttttaattg atttttaatg ctgttcaata
     9721 aaagtacttt tggtcttaaa gtgtcttctt tttctaaata tgcaatcatt gcatccataa
     9781 ttaaagaatc atattgttga ttttttccct cgacgtataa tttttttatt gcttctagta
     9841 atttaggatt ttcaaggaga gaagtatcag ttttgattag ttcttctata tcttgtatca
     9901 ttttaaaaaa tagcgattta ttatgaaggg aaaagtaaac ttcatcttct tgtttatgta
     9961 aaaacattga tttttcaatt aaaggattta gattatccat attttctaca actaaatcta
    10021 gaatttgtat agtaaatttg gcaataaagt atacatttat ttctcttttt tgtacatgaa
    10081 taatgtatat aaactgatga attagagcat ccaataagaa aactaaatca aatataattt
    10141 tttctatttt atcaccatag ttatcaaata agcttaattt gtattcattc aataagttag
    10201 ctcgtgattt tgtttgcaat ttacttgtag ccttacgttg ctcttcagaa aaattttctc
    10261 ttatataggt atttatggta gttctagaaa aaatattttt ccatacagca attatctttc
    10321 tgtttaactt ttctgatgct ggaatgtcat tattttcatt tatggaatgt ataatttcta
    10381 aaaacttttc atcataataa ttgattattt cgcagataaa actttcctta gtttcaaaat
    10441 gcttgtaaaa agtagcttta gacattttac actcttttac aacatccgat attgaagtat
    10501 tttgaaaacc cttttggtca aaaatttttt gtgcaatagt tataatgagc ctttgttttt
    10561 ctgtcacttt attatctcct ttcacgcaaa aacgtttagt actatagagt acctacaagt
    10621 tttaataaat atattataca tcatttggaa aaaaaatcaa tatatagact gtaatatgaa
    10681 atttttgaaa aatttgttgt tatttttagt cgtataaaaa agaaacttaa gagtttctta
    10741 agaattgttt aaagaagttg caataaatta gtggtatgat tatagtatga aagagaatgt
    10801 cttgaaattt ttctgtatca aattccaatt atggaggtgt agtatatgaa aaagaaaatt
    10861 tgtgtagcaa taatttttgc tctatttata gtttttgtag aaatgtttat tgatagctct
    10921 agcgaaggaa gtaaaattat gacagctgaa ttaagtaaat acaggaaagg cgtaaatgta
    10981 ttatcaataa acaatattat agtagcgaat aaaaaatata gtttgcccaa agattattct
    11041 cctcaggaaa gcagtgaagc gagagatgct ttttataaaa tgaataaaga tgctcaaaag
    11101 tcaggattaa atttaaaagc ttttagtaca tatagaagtt atgaatatca agatagatta
    11161 tttaagtcat atgtaaaaga acatggagaa aaagaagcaa atagattttc agctaaacct
    11221 ggagagagtg agcatcaaac tggacttgct tttgatattg gaggagatga ccagtcttgt
    11281 tgggcaaata aaaagttcaa caatacaaaa gaagctaagt ggttgtatga aaatgcatat
    11341 aaatatggtt ttatacttag atatccagaa ggtaaagaga atataacagg atatatgtat
    11401 gaatcttggc actatagata tgttggaact gaacatagca aaaactttgc aatgaataat
    11461 ttgactttag aagaatattt acatataaat taagagatgg tttgttaaaa ttaggttaaa
    11521 ataaaaaata acctagacta ttagctgtgt aaagtgatat aatgtaaata caatttagat
    11581 aactactcga aggggagtag cttacacgaa agtggtaatt aatcgtcaag acagtagaaa
    11641 tactcggtta attagcaagg gattccttga tagcaagacc ttcaaaataa gcattaggta
    11701 tgtttgtttt ggggtctttt tttattcaag gaatttttat ttaaaaaaat ttaaatttca
    11761 agtatacata agagccccca aacataaaat aaatttattg aagaggaggg caacaaatgt
    11821 cttacaaaag tacttatcaa gaagtaatta aaaatttaga ctcaagttta tctggtttga
    11881 gtaaagaaag agcagaagag ttgttagaaa aaaatggagc taatgaatta aaagaagcag
    11941 ataaggttcc tacttataag cttttcctag aaagttttaa agacccatta gtaataattc
    12001 tattgatagc tgctttagta caaatatttt taggtgaaac agtggagtct ataataattt
    12061 ttgcagttat tattataaac tctgttctgg gtgttgtaca aacaaaaaaa gcagaaagtt
    12121 cattagaaag tttgaaaaat ttatctgcac caaacgcaaa ggtaataaga gacaataaaa
    12181 aaatgacaat tccagcaaaa aatctagtag ttggagatat tgtattctta gaagctggcg
    12241 attacatacc agcagatgga aggcttatag aagcacaatc tctcaaggtt gtagaaggga
    12301 tgttaactgg agaatcagaa ccagttttaa agcatacaga aaaaataagt gaagatgtaa
    12361 gtttaggtga ccagaaaaat atggtattta gtggtgctgt agttgtttat ggtagaggtt
    12421 cttttgtagt gactggaaca ggtatggata ctgaaatggg taaaatagct gggcttttag
    12481 aaagtgctga aaataaactt acaccattgc aacaaaaaat agaggatttt agtaaaaaac
    12541 ttggtgtagg tattgtaata cttgctcttt taatatttat aattcaagta gcaagaaatt
    12601 actttatggc tggaaatgga gaaatgacac aaattatttt agattcattc atgttctctg
    12661 tagcagtagc agtagcagct ataccagaag ctctatcatc aatagtcact atagtattgg
    12721 ctgttggaac aaatagtatg gcaaaaaaac aagccataat aagaaaatta ccagcagtag
    12781 aaactttagg ttcaacaagt gtaatatgta ctgataaaac aggtactctt acacaaaata
    12841 aaatgacagt agttgattat tatatgtatg gaactaataa tgatgagtta gataaagaaa
    12901 aagtaaacta ttcttatcat gctaaattat tgactatggt ttctacattg tgtaatgact
    12961 ctcatataac tagtgatggt aaagaagttg gtgacccaac agaagtagct ttaataaatt
    13021 attcaagtaa aaatgattta gattatgata aattaagaag taagtatatc agaataaatg
    13081 aaataccatt tgactcagac agaaagctta tgtctactgt aaatagtata tatggagatt
    13141 atataatgtt tacaaaaggt gctccagata ttgtgtttag tagatgtaaa tatgctttga
    13201 aaaatggttc aaaagtagat ataacagatg agataataga agaatataaa cataaaaatg
    13261 aagagttttc aaatagagca cttagagtat tagcttttgc aataaaagat gtcccagaag
    13321 aagattttat accttcaatt gatgacgaac atgacatgac attggttgga ataatggcaa
    13381 tgatagaccc accaagagaa gaagtaatgg atgcagtaaa agaggcaaag agtgctggta
    13441 taaaaactgt tatgataact ggtgaccata aaacaacagc agcagcaatt gcaaaagaga
    13501 ttggtataat ggaagaagga gacttggctc ttactggaaa agagcttgat gctttgagtg
    13561 aagaacaact atatgaaaaa ctagaaaata taagtgtata tgccagagtc tcgccagaaa
    13621 ataaaattcg tatagtaaaa gcatggcaac ataagaataa tataacagca atgactggtg
    13681 atggtgttaa tgatgcacct gcattaaaac aagcagatat aggaatcggt atgggaagtg
    13741 gtactgatgt agcaaaagat gcttctgcta tggtattggt tgatgataac tttgcaagta
    13801 tagtaaatgc tgttgaagtt ggtagaacag tatacagtaa tataataaaa tcaattactt
    13861 atctatttgc aggaaactta ggagctatag tatccatagt atttgcagtg tttgcaggtt
    13921 ggtcaaatcc atttacagct ttacaacttt tgtttataaa cttagtaaat gactctttac
    13981 cagcaattgc attagggttt gaaaagtcag aaaaaaatat aatgaataaa cctcctagac
    14041 acccggatga aagtatacta gcaggaggaa cattgaaggc agttgctata agaggttgta
    14101 taataggtgt agttacaata attgcacaat atataggatt acaagtatct ccagaacttg
    14161 gagtagcaat ggcattttca acattgatat tagctagaat aatgcaaaca ttacctgcac
    14221 gttctaataa tcaaacagta ataaaattag gttttttcag caataaatac gttataggag
    14281 cagttttagt ctgtcttggg ctttactctt taacactaat accagtgcta agaggaatat
    14341 tctctatacc aacaacgttt ggattgtacc agttggggat ttgcctaggg ttagcattaa
    14401 tttcatcaat attaatggaa cttaacaaat taataaatag aagtgaagta aaataatcaa
    14461 aaaattaaag ggcatattga ttttaacaat atgcctttta taaatagtct aatttattct
    14521 atattatcga ttatttggaa tgcttttata acttcaggta attctttttc ttcaggcatt
    14581 ctaaatctta tgaaattttt atcaagtcca gtaaaatcac ttccagatat aactcttatt
    14641 tttaatttag caaactcttg ttgtaaatct atgtccttgt ttttatgttc aactgtcata
    14701 attgatacag tatcactagt ttcagctata ttaaggtttt tccatggaat taataattga
    14761 ttttttatat cttttgtttt ttcttttaat tcttctataa attgaacatc atctaataaa
    14821 tttgctgcaa tactacgaga tatttcagac acttcgtaag gagtagaaat ctttttaata
    14881 gggctaacaa gttgttctgg aagaactgca tatcctgctc ttagtcctgc taaaccgaat
    14941 ccttttgaaa atgtttttaa agcaattaca ttatcataat tatttagaag cttgacagct
    15001 gaattttctt ttggcatgta ttcaccataa gcttcatcta ccataactgc tatatcatat
    15061 ttagctgctt cctttactat attttcaatt gatgataaat gtataatttg tcctgtagga
    15121 ttgtttggat tgtctatgta aattacttta tattctggat ttattttttc aataaattct
    15181 ttttcattaa acttgaaatt atcctctttc tttaaaagaa cataatcata agttgcacca
    15241 tgcattttta tatctgtctc atattctgag aactgaggtg aatatcctaa aactttatct
    15301 cccttttcaa taaatagtct atttaaaagg taaataacat gtatagaacc atctgccaaa
    15361 caaattctgt cagtatctaa tgctataaaa tctttccaat aatcgactat attatctttt
    15421 aaagcaattg aatgaggata tcctcttatc atatcaaatt ttaaagcatt aaaagcttct
    15481 acagctttgt ttgagtaaga cacggtattt ataccttcac cacagtctat ctcgaataca
    15541 tcttctgaat gtccagattc tattgcataa ctttttttca actgcatttc aatagctttg
    15601 ttaattttta gtttcatact ttatcactcc ttatttagtc aataataaat gttataaaag
    15661 ttgctagatt taaaatatta tgaaaatata taatagtaaa aaatgaatat aaatattata
    15721 atagctcact ttatttaata tgattatata tcatatattg taaaatttct agcattataa
    15781 gactataaat tgaaaatatt cagaaaaata ctacaagaaa cgatttattt tgctcttaaa
    15841 ataagttaaa tgaatgttat taattttgta aattgaaata gtttaagtat aaagttatga
    15901 tataatctaa taagggtaat tctttagatt atattaagga aatataggta tacaataaaa
    15961 agttatggag ttggattatg ggatataaat taattgtaac agatatggat ggaactttat
    16021 taggcaataa tcataaagtt acagatgaaa ataaaactgc actacaaaaa gttataaaaa
    16081 gcggtattaa tgtaacttta gcaacaggaa gagccttcga ttcagctaaa tgcaatgtag
    16141 attttcttaa agaggacatg ccaataatag cttgtaatgg ttcattaata agagagcaaa
    16201 atggaaatat aatatattca aataagattg atacaagaac atgtttaaac attttagatg
    16261 tattagataa atacgatatt tactaccaat gtaatagtat agattctatg ctttcgaaaa
    16321 aaatagaagg acgtgaagat agattaagtg tatttttagg ctctgaaaca gaagtaattg
    16381 taaaagatga tttgagagaa gaaatcttta aaaaagatat actaaaattt gtaatcatag
    16441 aagaaaaaaa tccatctatt ttagatgaaa taagaaaaga attgagaaaa gttcaaggaa
    16501 taaagataac ttcttcatgg ccaaacaata ttgaagtcat gaatgaaggt gttgataaag
    16561 gtaatgcagt taaaatttta gctgaaaaaa tgaatataga cagagaagat ataattgcat
    16621 ttggagacaa ttacaatgac atagaaatga taaagtttgc tggtcttggg gtagctatgg
    16681 gaaatgcaga agaattgatt aaacaagaag ctgattatgt tacagataca aatcaagaca
    16741 gtggagttgc aaaggcaatt tataaattcc tagagttaaa agaatcactt agctcttaaa
    16801 acagtatata ataggaaaaa ataggttgac tattttgtag atagtaatta taatacaaat
    16861 agataaaaca taaaaattta gattcagtga aaaagagagt agatatagag aaaatctcag
    16921 cgaattgggg gatggtgtga gcctaatgat aacactttat attgaaaaga actttggaga
    16981 atactacttg aaattcagta tggtagtagg taatctcgcc ttaagagaat agagtgggta
    17041 aacattgttt accaactaga gtggtaacac ggaatatttc gtctcttggt ctttttgacc
    17101 aagagatttt ttagtttcat tgagttacaa gacttataat taaaatacaa aaaattaaaa
    17161 tatgaggtga aagttatgaa attatggggc ggacgcttta gaaaagcgga aaatcaactt
    17221 atggaagaat ttaataaatc ttttggctat gattgtgtac tgtacaaaaa agatatcgaa
    17281 ggaagtgtag cacatgttca tatgcaagta aaatgtggac ttttaacaga agaagaagga
    17341 aagtctataa cagaaggttt aaaagggata cttgaagatg tagaaaatgg aaagttagta
    17401 ttggatggag agtatgaaga tatacatagt tttactgaga taaatttaat tcaaagaatt
    17461 ggagatgttg gcaagaagct tcatacagca cgtagtagaa atgaccaagt tgcagttgat
    17521 atgagattgt atgctaaaga aaaagcaaat gatttggtgg gtttaatatc tgaatttaaa
    17581 gctactataa aagatgttgc tgacaaaaac ccagtgatga tgccaggcta tactcatctt
    17641 caaagagctc aagtagtgac atttaagcat cacttaatgg catattatag tatgtttgat
    17701 agagatgaga aaagaataaa aaatgctata gaaatattag atgaatcacc tttaggctgt
    17761 ggagctttag caggaactac gcatgatata gatagaagta taacttgtga acaattagga
    17821 tttaaaaaag tagtagataa ttttatggat ggagtaagtg atagagatta tttactagag
    17881 ctaatgagtg atttttcaat tataatgatg cacttaagta gattaagtga agagttgata
    17941 ctttggagtt ctcaagaatt tggatttgtt gagatagatg atttatatac aactggaagt
    18001 agtataatgc ctcagaagaa aaatccagat ggtgcagagc ttataagagg taagactggc
    18061 agagtatatg gaaacttatt tggtcttttt acagtaatga aaggaatacc attagcatat
    18121 aataaagaca tgcaagaaga taaagaaggt tttttcgata gtgtacatac tcttgaaatg
    18181 tgtatacaaa taatggatag aatgatagcc acactaaagg taaatgagga taaaatgaaa
    18241 caagcagtaa aaaatggttt tttaaatgca acagaagttg cggattattt ggttaaaaat
    18301 aatgttgctt ttagagatgc acatggtata gtaggaagta tcgttatata ttgtgaagat
    18361 aataaaaaag caatagaaga tttaacatta gaagaattac ataagttttc agatgctttt
    18421 aaggaagata tttatgattt tatcgactat gagagtatat taaataaggg aataaaaaag
    18481 aatttaaaat aatgtattta tttttaaaag ctgtaaattt gaatgttatc atttaaattt
    18541 acagcttttt attgtgtaag attttattat ataaaagtaa gaaacttaaa agtagtagta
    18601 tttaaaataa atacaaataa aaaattgtta ttaaaatgat aattaatatc aataagtatt
    18661 gacaaaatta tttctaaata ctattatata taatataaat aataatgaaa atcaatatca
    18721 aataaatggg aggaaatcta atgaacttat tagtgattgg tggtcatgaa agaatggaaa
    18781 aagattatat ggttatggcc aagaaaaaag gttataagac aaaggtatat actaccatgt
    18841 catctaaatt aaataattct ataggaagac cagatgcagt tgtcatactt acatcaactg
    18901 tttcacataa gatgtctaga acggttgagt ctcaagctaa aaaacaagat atattaatcg
    18961 taaggcataa aaatagtagt aaggtagcat ttaatgaatg tctagatatg gttgatgaat
    19021 gtctgggaaa ttgttcaaaa tgtaagctta aagacaaaca aaaacaaaaa aatatatagg
    19081 tataaaaaat acataaacat aaaattaaaa gtactgagat aatagttata attattatct
    19141 cagtactttt agatttataa ggataagtat attataaatt ttttattata atttccaact
    19201 acttgttact ttttcatatt ttttttactt ctagcttttt ttcttttttt cttctttata
    19261 tttgaagcta ttataaataa aactaaaagc actagaatac ctgcaataat cttaacaaaa
    19321 gtcattaaaa tattatttcc agtaaaaaat ggaagactgc tatcaagatt attttttgca
    19381 attaagtcta tactgttttc tagtttacca ttattatata catttaaagt accaacctta
    19441 gccccttttt ttataggcaa atcaatttta tttaactctg ttttggtatc ataattgcct
    19501 tgagattcat ctttttctaa gaatatccta taactatttt ttggttcata aatcaattca
    19561 tgttgttttg taaacaatac ttttttagta tctgtgtatt tttctttatc aacaatagta
    19621 gcacacttaa agttatcaaa tccataatca atcagaatac gtgagtctaa atataaatca
    19681 gcattagttg aattaaatac agcaacgata actcttctgc catcttttac agcactcgat
    19741 aaaagacatt taccagcagc atcagtatat ccagttttta taccatcaac tatatcatat
    19801 tttatattta taggttgacc tttgtatgta atcttatcat gtgatgtcaa aaacttatta
    19861 gtgtttacaa agtatctctc ataaggatat gcttttgtag cttcaaattt aagtgactta
    19921 gtttttacaa tttgtctaaa agtatcatta ttcatagctt ctctagctat gagagccata
    19981 tcatgagctg ttgtgtagtg atttgggtct ggtaatccat gaggattatt aaaatgagta
    20041 ttagtagctc caattttttt tgcctcagag ttcataaggt caataaattt ttgtacattg
    20101 ccaccaccaa catatctagc taatacaaaa gcagcatcat tagcagaatg aaccaaaaga
    20161 gcatctagta actgctttac agtaaaagat tccccatttt ttagatacat actagagcca
    20221 tctatttgag gtaaatcttt aatttctatt acttgattta aatctttaac ctcctttaga
    20281 actaaacaag ctgtccatac tttagttgtt gaagctggat aaagcttaga atttccatcc
    20341 ttattatata gtattttgcc agactcatag tccattaaaa ctgcatgttt agcaactata
    20401 gctggttcat tatctgcaaa agagatatta gagcttccta aaaatgaagt aaatatcaat
    20461 aaacagatta ataaaagtga taaatttctt ttcattgtga gcctccaaat tttaaattca
    20521 tcttttagta tagcatatat ttgtcaataa ttaaaagaaa ggagtgatta atatatgaaa
    20581 aaaaaagtaa aatttatttc gttttttgtt ttatttattt taataatatc atcaataatc
    20641 gtttataaat cgcttatttt caaagatgat atttatgttg taagtaaaaa aaatttacct
    20701 aatgaaaaaa cacatgttgt agagaatgaa attaaaggtg aagataataa tcacaataaa
    20761 gtaactaaag acatagataa aaattttcaa aataataaaa taactatata cataagtgga
    20821 gcagtaaata gacctggaat tgtaactata gagtcagaca agagacttta tgatgctgta
    20881 gaattactag gaggaacgac aaaagaagca gatttaaatg gagtcaatct atcagtaaga
    20941 ttagaagatg aacaacatta cataataccc aaaataggtg aagcaacatc tgttacaagt
    21001 aatgatgatt ccaataaacc aaatcagagg aatgagtcaa gaggtgagcc taaaaataaa
    21061 tccaataaaa atatatctaa cgaatcaaaa gtaaatataa atgtagcgac tatagaagag
    21121 ttggattctc tacctggggt tggagaagct acagccaata aaattttaca acacagagaa
    21181 gaaaatggac agttcagttc aattgaggaa ataaaaaatg taaatggtat tggagataaa
    21241 aagtatgaaa atataaaaga tttaatttgt gtagactaaa atataataca aaacttacat
    21301 atagatatgt tattataaat atgtatgtat atgtgtgtac atgtggaaat ttagtaaagg
    21361 aggcactttt atgaaaaaag aacaaaaaaa acttacagag atgacaacag caggtggttg
    21421 agccgctaaa atagggccag aggttctggc atcagtttta tctcaattgc ctaaaaatga
    21481 taatatagaa aatttattag taggactaga tacagcagat gatgctgcag tttataaatt
    21541 aaatgatgat atggcactta tccaaacttt agacttcttt acaccaatgg tagatgaccc
    21601 atacgtattt ggtcaaattg ctgcatcaaa ttcactatca gatgtatatg ctatgggagg
    21661 caaacctttg gttgctatga atattgtttg ttttccatca tgccatgata tggatgtact
    21721 tgcagaaata ttaaaaggtg ggtttgataa agtgaaagaa agtggagcac ttttggttgg
    21781 agggcatact gtagatgaca aagagcctaa atatggtctt tcagtttctg ggattgtaag
    21841 tccaaataaa gttttatcaa atgctactgc aaaacctggt gataagttaa ttataacaaa
    21901 accaattgga gtgggtgtat taaatacagc catgaaagaa ggtatggtag aacaacatat
    21961 agcagataaa gtaatagaaa taatgataca tttaaataaa tatgcagcaa tgagttttga
    22021 gaaatttgat gtaaattcgg ttacagatat aactggtttt ggtctacttg gtcatacttt
    22081 agaaatggcc aaagcttctg aagtaagtat tgaaattgaa tcaaaacatg taccaattat
    22141 tgaaggtgca atagaaatgg cacaaatggg aattatacct gctggtatgt ataaaaatat
    22201 gcattatgta tctaaagatg tagaagttgt aggaaatata gaagttgcag ttcaagatat
    22261 tttatacgac ccacaaacat caggagggtt gttaatttca gtaaaagaag aacttgcaga
    22321 ggaacttgta aaagatatga aattaaatgg agcaatagaa gctaaaatta taggttctgt
    22381 agttccaaaa ggggaaaaat atattacagt tttataaaat attatagaga aagagtgatt
    22441 gttttgagca aaagagaatt gtttgcaatg ttgccttcag ttgatgaagt attaagtgat
    22501 aatagaattg tagaaataat aaatgaatat cctagaagtt tagttttaga atctgtgaga
    22561 gaagtaatag atttaaaaag acaatttata ttaagattaa aagaagatgc atcaaactct
    22621 gtaacaatag agtttggtga aataatagaa tctgcaatag agagagttaa gttaaattac
    22681 tcattatctt taaaaaaagt tataaatgca actggtacag ttattcatac aaatttagga
    22741 aggtctttac ttagtgaaga tataaaagat gaattatggt gtgctgcttc aaggtattca
    22801 aacttagaat acgatttgga taatggagaa agaggttcaa gatactctca tttaactagt
    22861 acaataaaaa gacttactgg agcagaagat gtactagttg taaataataa tgcagcggca
    22921 gtacttcttg tacttagcac aatggctaaa ggaggagaag caattgtatc tcgtggagaa
    22981 ttagttgaag taggtggttc atttaggatt ccaagtataa tggctcttag tggtgcagaa
    23041 ttagttgaag ttggttcaac taataaaact catttgaaag attacaagga ggccattaca
    23101 gaagatacaa atgtattgat gaaggttcat acaagtaatt atagaataat gggattcaca
    23161 gagagtgttt ctatagaaga attggttaat ttaggtaaaa aatataaact acctgtaata
    23221 gaagacttag gaagtggagt ttttatagat ttatcaaaat atggtttatc ttatgaacca
    23281 actgtattgg attctattcg acaaggtgca gatgtagtaa cttttagtgg agataagatg
    23341 cttggaggtc cgcaagcagg tattatagtt gggaaaaaag agtatataga aaaaatgaag
    23401 aaaaatcaac ttactagagc acttagagtt gataagctta caatatgtgc tcttgaagca
    23461 acattgagaa tgtatttaga tgagacaaaa gctatagaaa atatacctac tttaaaaatg
    23521 ttgacatata aaattgaaga attagaagta aaagcaaata aactatttga aaaaataact
    23581 gctttaaact taaatgcaaa cataaatata gaagatggat tttcacaggt gggaggaggg
    23641 tctatgcctc ttgaaactat aagtacaaag gtaatatcaa taactcctga acatatgaat
    23701 gtttcatctt tagaaaaaaa actaagactt agtgaagcac atataatagc cagagtgtat
    23761 gataataaat atgtactaga tgtaagaact atatttgatg atgaatttga tgtaatagtt
    23821 gaggaattga gaaaagcatt taattaaata aatttgagtt aataaatatt aaatttagaa
    23881 gtataagtat gaagttttga ttaaaaaata gctacacaca cttgtgactt tagtcatagc
    23941 aggtttagag ggggcaaaaa atgaaaaatg tagtaattgg aacagcaggt catattgacc
    24001 acggaaaaac tactcttata aaagcattga ctggaagaga gactgatacg ttagatgaag
    24061 aaaaaaagag aggtatttca ataaacttag gatttactta ttttgattta ccaaacaata
    24121 gaagagctgg aattgttgat gtcccaggac acgaaaaatt tataaaaaac atgttagctg
    24181 gagcatcagg aattgatata gttttacttg ttgtagcagc agatgaaggg gttatgccac
    24241 agacagttga gcatttagat attctaagct ttttaaatat caaaaatgga atagtggttc
    24301 taactaagtc tgatactgta gatgatgaat ttagagagct agtaaaagaa gatatttgtg
    24361 aaaaaataaa aggaactttt cttgaagatg ctgaaataat tgaagttgat tctatatcta
    24421 aaagaggtat agacactctt attgaaaaaa tagataaaat gagtgatgat atagaagata
    24481 aaaatgaaaa ctctccagca agacttaata tagacagagt attttcaata aaagggtttg
    24541 gtactgtaat aacaggcact ttaatagaag gaaagataag tatagaagat gatttggtta
    24601 tatatccaca ggtattaaaa acaaaaataa gaagtataca agttcatgga gaaaataaag
    24661 atacagctta tgcaggtcaa agaacagcaa tcaatatatc aaatgtaaaa gtggatgata
    24721 taaaaagagg agatgtttta gctgcaccaa actcattgga ggaatcaatg atgttagatg
    24781 taaaattatc attggtaaat catttagata aaggattaaa gcactgggat agattaagat
    24841 tgtatcatgg aactagagag atactttgta gagcagttcc actagataaa gaattaattg
    24901 aaagtggaga aagtggctat gttcagttga gacttgaaga aagcatagta tctaagaaag
    24961 gtgatacgtt tgtagtgaga cgttattctc caatggaaac tataggtgga ggtgtaataa
    25021 ttgacccatc tcctaaaaaa cataagaaat ttgatgaaaa agttatagaa gcattaaaaa
    25081 taaaagaaaa aggcgaacta aaagatataa tagaagagta cttaaaaagg aatttaaaaa
    25141 attatccaaa tataaaagaa ataatgagct atagtggtgc tcatgaagaa gatgtaaaaa
    25201 gagcgcttga aacacttata tcagaagata aagtatttat tataggaaat atgtatatgc
    25261 atataaatca atacaataaa ttaaaagaaa atacaataaa gcttttaagt gagtaccata
    25321 agaagtatag gcttagaaaa gggatactaa aagaagaagt aaggtctaag atagagagta
    25381 attttaagac tagggagatg gatattttat tagaaaaatt gtctacagaa aatgctataa
    25441 aaatagaaaa taatatagta tctctattag attttgaggt tattttaaat gataagcaaa
    25501 aagaaatagc aaaaaaaata gaaaaaagat taaaatcatg tggagtttct tctatactga
    25561 caatagatga agtttctgaa ggaaatcata actatgctga agtattggag tcaatgatag
    25621 gtaataaagt agagaaatta gatgatttgt atataatgga caaagatatt tatgaaaatg
    25681 caaagaacat tttgataaat tatataaaag aaaataaaga aataacatta ggagaataca
    25741 gagatttgat tgattctagc agaaagaatt gcatgattat attggaaaac ttcgatagaa
    25801 acaaaataac taaaagagta gaaaacaaaa gaattttgtt ttagcagaaa atgtctgttt
    25861 ttgctttaag gtataaagaa ggaattataa tgtcgagtat cgtataatta attagtataa
    25921 aatattaatt aagaggaagt gtaaagtatt gaagaatata atacctgatg taaaaccaga
    25981 tataaaagaa agagaagaca tgagcctttt atttataata atgggctcag tacttttatt
    26041 atttattata aaagatatag agaaatatga aactttatta catgccatat atacgatatt
    26101 tttagtagct acaggagtaa ttatatttaa tactacaaaa tttaaagtag attcattttc
    26161 tacattttta ggattaattt ttatagctac tggagtattg gagtgtatat atttatttaa
    26221 ttccttgggg attaaaacta agtctataat ggagattaac atcacaatat cagcaataac
    26281 agatttattt cctatactgg gtgtatactt aagttttaaa tttgttaagg ataacaaaca
    26341 aatttattca agtgtaattt tatttatagc aactgctaca ttaacaataa catctttatt
    26401 cataatatca ggaatttgtg actatctggg aaatggtaaa tcacaatatg ctttaggagt
    26461 tataatatct atattcataa taattatttc tatcattagt ggaattgaat taaattcaag
    26521 ttcaaataaa ggtaaatggg aatatgatga aaaaaagatt tttaacagaa taatagtaat
    26581 aataatctta tcaagagttc caaatctatt gcatatagtt attgataata gaaatgtaga
    26641 gaggatatta tcacaaataa taataaatgt agccttatat tatttatata attacatagt
    26701 atctaagaat attaagaaaa cagcactgga attgcatgat acgaatgaag aattaacaaa
    26761 aaaaacggaa agtttaaaag aaaagaataa aaaattaatc tatgagacag aaaagataga
    26821 ggaacttaag ggaatactta tgaaaagaga atctaggcta caatctacat tagatgtagc
    26881 agtaaatagc atagtagtat ttagcaaaga tggagaaatt acatatgcaa ataagagctt
    26941 tagaaatact tttggagact ataaagaaca agataaattg actgataaaa taaaaaactt
    27001 caatgatttg atagaaagca ttcattatgt gtttgttcat gagaagaatg cagagaaact
    27061 tattagtaca agcgataata aagtttatca agctatattt gcacctttaa ttattgcttc
    27121 acaaacagaa ggagtattgt gtgtactaga agataagact aagaaaaagg agtacgaacg
    27181 caaacttatt gaagcaaata agagaagtaa agatttttta gaaagtattg gagatggcat
    27241 agtagtgctt gaaggtaata acaaaatata tgttaataat gcatgtaggg aaatttttaa
    27301 aaataattta gagagtattg atttttcatt agtatgtaaa agtgaagaaa gtatagaaaa
    27361 aagatatgta atagatggtg aagtaaagta tgtagagatg agtttttctc agtatactaa
    27421 tgctggtact aataaaacta ttattgtaat acgtgataca acctctcgaa aaatatctca
    27481 aataaaactt gaggaaagtc aatcatcata ttcaagattt atagatatat tacctgatgg
    27541 catatgctta ttaaaagaag atttaagtat aaactatgca aataagtcgt ttttagatat
    27601 gctagctttt acaaacatag atgatataaa agattctaat attaaactat taatgaatgc
    27661 gaatactgaa gaaaaaatga aatttacgga taatatggct aaagttttaa atgaaaatag
    27721 aagtatactt ttactggaat atgaattgat aaatagttat gatgatatag tagaagtaga
    27781 attaagtgca ttaccttttg ctatatacaa tactagatac ataatgctta ttataaaaga
    27841 tttagtacat aagaaatatt ccgagcaagc agaaaaagaa ctgcttgaaa gatttaaaac
    27901 agataaaatc aaaacggagt tttttgcaaa tatgtctcat gaactaaaaa ctccactaaa
    27961 tgttatatct agttcaaatc aactggtaga ttctttctat agaaatgaaa agataaaaga
    28021 ttacaataac aatattaaat ctcatgtgga tttagttaga caaagttctt ataggcttca
    28081 aagattaata aataacatta tagatttaac aaagatggag tctgggttct atacactaaa
    28141 acttgcgaaa tacaatattg tatctgtaat agaagactta tttatgaata tagaagagta
    28201 tgcacttaga aaagacataa aaattctttt tgatacagac cttgaagaaa ttaatgttta
    28261 tatagacaag gttgaaattg agagaattat gttgaatcta ttgtcaaact gtattaagtt
    28321 tactgataat ggaggctgga tatatgtaag tattcattat aaaatagata aagtaatcat
    28381 aagtgtaaaa gacactggag ttggaatacc acaagataaa ttagagttga tttttgaaga
    28441 attttcacaa gttgataaga ctttatccag aaatactgaa ggaagtggaa tagggcttgc
    28501 tatagttaag aatctagtaa gtcttcatgg tggagatata gaagtagtaa gtgaagtgaa
    28561 taaaggcaca gagttcttaa tttcacttcc aatgaaggga tttagtagtg aacattacac
    28621 agaagataag agaatatata atattcaaga aaaaataaaa atagaatttt ctgatatata
    28681 ttattaaaaa tgattatgag caaaaataaa ataaaaatac tatttgttta aatatttaag
    28741 ttgtaagaaa taggctatca taaggtaaat tattatgata gcctattttt agctaggttt
    28801 tatatctata tgtgagaaca tataatctct aaattacctt caaaactaaa atctccaaag
    28861 tcgtttggta gtataaaatg catacccttt tttaaattgt attctacaga attgtgtata
    28921 agttttccat ttccatccaa cacactgtat aaagaaaaag gtgtattgtg tgtaaaatta
    28981 cattttccaa aaacatctaa tttataaaca ctaaaaaatt cgtttgacac aaatgttgtg
    29041 catttaaagt ctgatgttga tacgatttta tagtcagtat cgaaatttat atgaggaaca
    29101 ttcgtaacat ctattgattt ttgaacatga agttcacgct tattacctga attgtcagtt
    29161 ctatcataat catagactct ataagtagta tctgagtttt gttgagtttc taaaatcaat
    29221 gttcctttgc atatagcatg tatagtgcca cttggaacat agaaaaagtc tccctttttt
    29281 atattaacct ttctaagtag tttgtcccat tcattattat ttaccatatt tacaaattct
    29341 ttatgagatt ttgcattatg acctattata atttctgcat catcagaaca atctattaca
    29401 taccaacact ctgtcttacc taactctcca ttttcatttt ttttagcata ttcatcattt
    29461 ggatgaactt gaactgataa attatcattt gcatccaata tttttgtaag taaaggaaac
    29521 ttatcaccag gtgtgtttcc aaataattct ctatttttat tccacaattc agatagtttt
    29581 ttacctttat attttccatt ttctattaag caatctccat ttttatgaga gcttatagcc
    29641 caacattcac cagttgttgg cgaatctatt tcataattaa atttatcttt aagtgcagta
    29701 ccaccccata ttctatccat aaaaataggt tttaaaaata atggttccat aatcctgttc
    29761 ctcctatgat attaaattta tcctacaaat aattatatat aaatagatta taaaatcaat
    29821 agtaaaaaag ttttgtccct tgaaatgaca taaagtataa ggagttatta aataagataa
    29881 actatgatat ataatataaa gttctaaatt ggagtgataa ttatggagtt gtttaaggca
    29941 gaaagcaaaa aatataaaaa agaattttta aaattattgg agcaatgggt tagtatacct
    30001 tcattttatg atagaaaaac tgtatctaaa gacatgcctt ttggaaaagg agtatatgat
    30061 gcattaaact ggtttgaaaa tctaggtaga gaaaacaatt tcaaagtaaa aaatatagat
    30121 aatcatgctg ttcaaattga atatggaaat ggtaaagagt atgtagatat atttggacat
    30181 tgtgatgttg taaatccagg tgaaggatgg gatagtgaac catttaaact aaatattata
    30241 ggtgataaat tagttgctag aggtgtatct gataataaag ggcctatgat agtgaatttt
    30301 ttagcactaa aaatgataaa ggatttagat attaatttaa aaagaaaggt tagattaata
    30361 gctgggggaa atgaagagag tggatttaaa tgtataaagc attattatag caaagagcca
    30421 tatggtgtat gtggatttac tccagatgct aaatttcctg tattaaatgg agaaaaaggt
    30481 ggagcaataa taaagttaat ttcaaatata gacgataaat ctttatatat aagtggtgga
    30541 atagagttta atacaatacc agataaagtt tatataaaga atgtagaaaa attgggtaaa
    30601 gataatatat gttttgatat taacaatata tcgataaatt atgataatgg caattatatt
    30661 gtgtatggaa aaggtggtca ttcatctaaa cccgaaaaat caataaatcc tatattagct
    30721 acaattaaac ttttgtcaga aaatattgat gaaaaatgga caaaagattt atataagttg
    30781 ataaatcaag ataatataaa tggaaactta tttggtttaa atattgaggg gaaatgtgga
    30841 attttatcaa tggtgccaac aataataaat atagtagatg gaaaattaga agtagtttta
    30901 agcgttagat atccagaaat attaaccatt gaagatataa taaaaaaatt taatttgtat
    30961 atggagcaaa ataatattaa taaatttgag cttattgggg aaaatttaaa gcaagcaaat
    31021 tatatagata gaaattcaaa acttgtaaga agtttgcatg atatatatat aaagtattct
    31081 ggagatttaa aaaatgatgt tagagtaact agtgcaggaa gttatgcttc tgaaatgaat
    31141 aattcagtaa tatttggttg tgaatttcct gatggtagct ttggcaatgt ccatagtgcg
    31201 aatgaatttg ctagcttaga tagatttata actgcaatag gtatatatgc agaggcaata
    31261 attacattat gtaataaaat ataaatacaa aatgtccctt atatagacat attatatata
    31321 caattaaaat tttacttgat atataattga aatcaaagag aaggtgatac aatgataact
    31381 aagaaacatg caataatagt aaagagttta agcaataaag atggatatat gacatcaaac
    31441 gaattagcta tcaaattgga tgtgtctact aaaactatta aaagatatat agcagattta
    31501 aatagtgttt taagcaaata tgatttggta atagcttctt ctagaggtat aggatacaag
    31561 ttaagcggtt ctaaaaacaa tatagccaga gctgttaaag aagcaaataa atatattgat
    31621 ggttttttag atgactctga agagtcaaga atgtcaaata taatatgtat gcttattaat
    31681 agaaattata tgagtataga agcaatggca gaagaattaa atctaagtat agccgctata
    31741 aataaattgt ctagtaagct taaaagaaaa cttgaaaagt atgacttagt tataaaatct
    31801 aaaccttact atggaagcta tatacatggt gaagaaatta atataaggca attaattact
    31861 gactatgcaa taaaattaga tgaaaaaagt aaagtgaagg tgtttttaga tgacattagt
    31921 gaaaacgata tacactgtat agaaagtatt ttagaaaagc acttaagaga aaaagataca
    31981 ataatttcag ataaagattt taatcttttg ctttcaaaaa taatcgtttc tgtatttagg
    32041 agtaagagag gtcatagtaa caatataaac ttaatggata caagttatag atttcataac
    32101 tactacttta tagaaaatct aatgaaagaa atatctgata aaattggatt taaacttatt
    32161 gaagatgaag taatttatat ttcaaattat tctggtgtaa tagcctataa aggtacacaa
    32221 ggaagaaaaa acacaagtga aattgaggaa cgaataagta ctgttataag tagtgcactt
    32281 caagatatat tccttatttc aggtagtgat tatacaaaag atgatgagtt tatggttgca
    32341 atctctgacc atataaagag atttttaaat agagctagag ctaatgtaaa atcaaataat
    32401 cctttacttc atcaaataaa agagaagttt cctatagctt ttaatttagc cgtgttttta
    32461 tcaaataaac tagaaactga gtttaattta aaacttgatg aagatgagct tggatatata
    32521 gctatccact ttgctgcttc aaatgaaaga atgaaaaaaa atactagtaa gaaaatttgt
    32581 atagtttgtc attatgggat aggaacagga caacttattt ctgaaaagct aaaacagaat
    32641 ataagtgatt taagtgttgt gggtgtttat ccagttagat atctcgatat ggctataagt
    32701 caagatgttg atttgatagt atctacagtt gaattaaaag gatatgaaaa acctgtctta
    32761 tatatagaaa atatatttga tgatagctta attgaaaacg taaacaaagc cttttatgaa
    32821 aaggaagaaa gaagaaagat aataagtaac atgtttgatg aaaaagcttt tttcagtata
    32881 aaggctagta ctaaagaaga ggtcataatt tcactttcaa ataaactaaa agaaagagat
    32941 tttatagagg aaagttctat aaaaagtata atagatagag aaaatatttc atcaactgaa
    33001 ataggaaatc tagtagcaat accccacaca atagtgaagg gagataaaaa atctataata
    33061 ggagttggaa tacttgaaaa tccaataatt tgggataagc aagaggttca gctagtattt
    33121 atggtatttt ttaatactaa agaaaaacat aatttctcga tatttaaata cctttataat
    33181 tttataaaag atgaaggagg agtaagagga attattaaaa tatgtgactt taataaatta
    33241 atggcactta taggtaatta aggaggaaat taataatggg aacaaacata tttaacaaag
    33301 aatatgtatt tttaaatgtt gatgctaagt caaaggttga agttttaaaa tttatatcaa
    33361 aaaaagctaa agacttaaac ttggcagaag atgagagctt agtatatgaa ggtttaatgg
    33421 ctagagaaga acaatttact acaaatttag gagaatctat agcaatacct cacactaaaa
    33481 atgatgcaat tgaaaatcca gcagtagtag tattgaagtt taatgaagat gttgtttgga
    33541 atgaaggtga ggataaggtt aaattagcaa taagcttact tatgccagga aaaagtaaag
    33601 agaatattca tcttaaatta ttatcatctc tttcaagaaa attaataaat aaagaattta
    33661 aagattctct tttaaaaagt gataatgttg aagaaatatc taacttaata aatgaagctt
    33721 taggtttata attaaacact aaggttataa aataaaaaaa taattaacta aaaataggag
    33781 gaatttgaaa tgaataaaaa attagtagca ttatgtgctt gtccaatggg actagctcac
    33841 actttcatgg cagcagaagc aatagagcaa gcagcaaagg cattaggtta tgaagcaaaa
    33901 gttgaaactc aaggtgctga tggtgtacaa aatgagttaa caagagatga tatattagga
    33961 gcaactatga taatacatgc agtagcaata acaccagaag gtatggaaag atttgatggt
    34021 tgtgaagttt atgaagttga attacaagaa gctataaaaa atgcagaagg tgttataaaa
    34081 gaaatagaag aagatttagg aatataataa atttattgtg gaggaaatag aatggcaatt
    34141 aagaaaaaag ttataaacag ttcaggtgct tctacaacag gaggaaataa tgtaggaaaa
    34201 tctactccat ctagtccaac tcaatcaaag ggaaatggaa aatggaaaga aataagtaaa
    34261 catataatga caggtatatc atatatgata ccagttttag taatgggtgg acttataggg
    34321 gccttatctc aattaatacc atatgctata ttaggacttg acccatctgt aggtatagtt
    34381 gatgctatga actcaggaga atttacaggg ttcaagctta gtcttttaaa tatagcacaa
    34441 ttaatgtcaa atttcggatt tactttgttt ggatttgcga taccactgtt tgcagcattt
    34501 tgtgcaaact ctataggtgg aaaaacagct ttaattgcag gatttatagg tggatatata
    34561 gctaataaac ctgtaggtgt accacaattc gttgatggac aatggactga agttgttcca
    34621 gttgcatcag gatttttagg tgcaatacta atagcattta taataggata ttttgtaaaa
    34681 tggttaaata aatctataaa agtttctcat aactggttag cttttaaaac aacattttta
    34741 ataccattaa tagcgtcatt agcttgtatg gtattgatga tatttataat aactccattt
    34801 ggtggattaa ttaatgaaag tatgaaaaac ttcttaacag cagcaggagc agctggagaa
    34861 tatgtatatg caacagcttt ggcagcagct acagcattcg acttaggagg accaataaat
    34921 aaagcagcag gattcgttgc acttggttta actacagaga atgtacttcc aataacagca
    34981 agaacaatag ctatagttgt tcctccaatt ggattaggat taacaacatt attagataaa
    35041 agattagtag gaagaagagt atatgataga caattctatc aagcaggaaa aacttctata
    35101 ttcttatcat ttatgggtat atctgaagga gcaataccat ttgcacttga gagaccaggt
    35161 tttgttatac ctttaaacat agttggaagt gtaataggag ctattacagg tataattcta
    35221 ggagcaattc aatggttccc agagtcagct atatgggcat ggcctctagt agataactta
    35281 tttggatata taataggtat agcagttggt gctatattca tagcagtagg aaatatattc
    35341 tatagaaata aattaataaa agatggtaaa cttgttgtag attatatcga ctagtattta
    35401 atttttacta tctataaata actttggctg gtaatatggt tttaatcaat attaccagcc
    35461 ttacctatat gattaattaa aggcggtgga gataagatat ggataagata gaaaaagtaa
    35521 gagagtattt tagagaatat gatatagatg gatttcttat aaatagtcct acaaacaagt
    35581 tttatgttgg aaatttattt agctcttcag gttatgtttt tataactaaa gaatcacaat
    35641 atataatagt agattttaga tattttgaag aaattaaaag aaaatctagt ttgtttaatg
    35701 tagtattaat ggacaaaact agaactcatt ttgatataat taatgatatt tgtagagagc
    35761 agaatataaa ggaaattgga tttgagggaa atgaagtatc ttttgattta tatagaagta
    35821 tgagtaataa gttaagtgca accttaaaat cagttgattt atctacatta agggaaacaa
    35881 aaaatgaaga cgaaataaaa tatataaaaa aagcttgtga aatagttgat gctacatttt
    35941 atcatatagt agactttata aaggttggta tgactgaaaa gcaagttgaa aatgaaattg
    36001 ttagaattat aaaagattta ggtggtcaaa aagaatcttt cgatacaata gttgcatctg
    36061 ggcttagagg agcattacct catggaaaag caagtgaaaa agttatagag tatggagatt
    36121 ttgttacctt tgactttggt gctaagtata ataattattg ttcagatatt accagaacca
    36181 tttgtatggg tactataaat aaagagttag aagaaatata taatatagta agaaaagcta
    36241 atgaagagtg tataagggtt ttaagaccgg gaatgactac tggagagata gataaagttg
    36301 caagagacat aattggttca tatggatatg ccaataattt tggtcataat ctgggtcatg
    36361 gtgtaggaat tatggttcat gaatatcctg cattagctcc tgaatcaaat gaagtattaa
    36421 aagaaggaat gattgtaact atagagcctg gtatatatgt tccaagtctt ggaggcgtaa
    36481 gaatagaaga tgatgtactt ataacacaag atggatgtat gagattgact acatctacta
    36541 aggatttaat agttgtaagc taaaaataaa ttagataaat attttataac aataagaaaa
    36601 gaagaggtaa atttaaatgt ataattttga tgataaacca gatagagtaa gtgaaaagtg
    36661 tagaaaatgg gatttaaata ttataagaga taaatttgga gatattagag aagattttat
    36721 accaatgtgg attgctgata tggattttaa aataccaaca gaaatagaaa acaaatttat
    36781 tgaagctgta agaagaggtg tatttggata tacatactgt tatgatgaat tttatgattc
    36841 agttattggt tggcaaaaaa atatgcatga ggtaaatgta gaaaaagaat ggattacatt
    36901 aacttatgga actgtatcaa cacttcatta tgtagtacaa gctttttgta agaaaggtga
    36961 tagtataata ttaaatacac cagtttatga cccctttgaa tcatcagcaa aaaaacaagg
    37021 tgtaaatgta atttgtaata cattagatgt tgtaaatgat agatattata taaattttga
    37081 taaattagaa gttcaaatta aagaaaataa gcctaaatta atgatgtttt gtacaccaca
    37141 taatccatct ggaaggatat ggacaattga agaaatgaca agggtagcaa ctatatgtaa
    37201 agaaaataat gttattttgg ttgcagatga agtacatgca gagcatattc attatggtac
    37261 atttaattca atacttaaga ttggaaaaga gcttttagaa aatgttattc tattgacatc
    37321 accaaataaa ggatttaatc ttggtggatt aaagacttct tattcaatag ttataaataa
    37381 ggatataagg gacaaattta gaaataaatt aaagcaaaat tctataactt ctccaaatgt
    37441 gtttggaata ataggattga taaccgctta taatgaatgt catgagtggt taagtggtgt
    37501 aaatgaatat ataaaatcaa attatgaact tttagaaact tgggtaaata aatataataa
    37561 gataaaattg atgaaaatgg aatcatctta cttagcttgg atggatataa gtggattagg
    37621 tattagtgca agtgagttta cagataaatt ggctatagat acaggagtat tattagaaga
    37681 tggaagtcat tttgtaaaag atggtgagaa gtatgttaga ataaatttag gaactcaaaa
    37741 ggaaaatgta attgaagcct taaatagaat ggatttattt ttaaagtctt tataaaaatt
    37801 tggttgaatt agtattttat ataaatttaa actattaata tggtatatac ataagtagaa
    37861 tttgttttag attacttaag tatataccat attttatata aaaattaaat ttgcattagt
    37921 catg
//
LOCUS       DBGJFU010000023        37764 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.023, whole genome shotgun sequence.
ACCESSION   DBGJFU010000023 DBGJFU010000000
VERSION     DBGJFU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.023"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            161..679
                     /locus_tag="ACOIAS_001152"
                     /pseudo
     CDS             161..679
                     /locus_tag="ACOIAS_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
     gene            1111..1365
                     /locus_tag="ACOIAS_001153"
     CDS             1111..1365
                     /locus_tag="ACOIAS_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700871.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1401..1724
                     /locus_tag="ACOIAS_001154"
     CDS             1401..1724
                     /locus_tag="ACOIAS_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HGZ2700872.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIF
                     IDYII"
     gene            1998..2150
                     /locus_tag="ACOIAS_001155"
     CDS             1998..2150
                     /locus_tag="ACOIAS_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700873.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2419..3267
                     /locus_tag="ACOIAS_001156"
     CDS             2419..3267
                     /locus_tag="ACOIAS_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HGZ2700874.1"
                     /translation="MWILVPIITIVLLIIAVSSMQYILVMIAFLLIIYSFIEKKIVMG
                     LVSVLFFTYSIYLCATWEDKSLIADNKVETVKAQRETVEREKEMERRRIQEEVDKERY
                     IEKHGMEISENDLKVKLEALVPQEYKGKKYELKVGKFKRYSMYFDLTVQNEKFSNSEE
                     CKKFVKEIANDLKKIKISKAYFKFHSKDDGGIYNSVYIDYFRNIQNNVDNVENLEFNE
                     FELKTEEEEKREQEKIEQEKNSYNNYIQNRVVDPLDRIKKLKELLDSGAITQEEYNKK
                     KKELLE"
     repeat_region   3534..4214
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3534..3561
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            4370..4558
                     /gene="srl"
                     /locus_tag="ACOIAS_001157"
     CDS             4370..4558
                     /gene="srl"
                     /locus_tag="ACOIAS_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Srl"
                     /protein_id="HGZ2700875.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4678..5043
                     /locus_tag="ACOIAS_001158"
     CDS             4678..5043
                     /locus_tag="ACOIAS_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HGZ2700876.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVVNCLDIKNVNENIRL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQGWISDEV
                     LPSIRQTGAYINNNAIPKS"
     gene            5040..5492
                     /locus_tag="ACOIAS_001159"
     CDS             5040..5492
                     /locus_tag="ACOIAS_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700877.1"
                     /translation="MREKASEIEKLQLVYNSTSMLKELLDDAGFDNKSKLLTAKTLYK
                     KAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVW
                     EINGSWTGTVNKYTKSVIDKVRTWIEENNRPTKIAGEKKNYYVFYKIE"
     gene            5544..5705
                     /locus_tag="ACOIAS_001160"
     CDS             5544..5705
                     /locus_tag="ACOIAS_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700878.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            5832..6005
                     /locus_tag="ACOIAS_001161"
                     /pseudo
     CDS             5832..6005
                     /locus_tag="ACOIAS_001161"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
     gene            complement(6034..6498)
                     /locus_tag="ACOIAS_001162"
     CDS             complement(6034..6498)
                     /locus_tag="ACOIAS_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700879.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(6634..6951)
                     /locus_tag="ACOIAS_001163"
     CDS             complement(6634..6951)
                     /locus_tag="ACOIAS_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700880.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7234..7530)
                     /locus_tag="ACOIAS_001164"
     CDS             complement(7234..7530)
                     /locus_tag="ACOIAS_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700881.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITNEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(7549..7962)
                     /locus_tag="ACOIAS_001165"
     CDS             complement(7549..7962)
                     /locus_tag="ACOIAS_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700882.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7946..8179)
                     /locus_tag="ACOIAS_001166"
     CDS             complement(7946..8179)
                     /locus_tag="ACOIAS_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700883.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8398..8772
                     /locus_tag="ACOIAS_001167"
     CDS             8398..8772
                     /locus_tag="ACOIAS_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2700884.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            9384..10076
                     /locus_tag="ACOIAS_001168"
     CDS             9384..10076
                     /locus_tag="ACOIAS_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2700885.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            10272..12332
                     /locus_tag="ACOIAS_001169"
     CDS             10272..12332
                     /locus_tag="ACOIAS_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HGZ2700886.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12538..12861)
                     /locus_tag="ACOIAS_001170"
     CDS             complement(12538..12861)
                     /locus_tag="ACOIAS_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HGZ2700887.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12854..13555)
                     /locus_tag="ACOIAS_001171"
     CDS             complement(12854..13555)
                     /locus_tag="ACOIAS_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HGZ2700888.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13833..15218)
                     /locus_tag="ACOIAS_001172"
     CDS             complement(13833..15218)
                     /locus_tag="ACOIAS_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2700889.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALVEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(15664..16086)
                     /locus_tag="ACOIAS_001173"
     CDS             complement(15664..16086)
                     /locus_tag="ACOIAS_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HGZ2700890.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            16564..19467
                     /locus_tag="ACOIAS_001174"
     CDS             16564..19467
                     /locus_tag="ACOIAS_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2700891.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISVSEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            19500..19946
                     /locus_tag="ACOIAS_001175"
     CDS             19500..19946
                     /locus_tag="ACOIAS_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2700892.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            20069..20371
                     /locus_tag="ACOIAS_001176"
     CDS             20069..20371
                     /locus_tag="ACOIAS_001176"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2700893.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            20552..21958
                     /locus_tag="ACOIAS_001177"
     CDS             20552..21958
                     /locus_tag="ACOIAS_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HGZ2700894.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            22302..23360
                     /locus_tag="ACOIAS_001178"
     CDS             22302..23360
                     /locus_tag="ACOIAS_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGZ2700895.1"
                     /translation="MGDKMRASVLYSVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            23459..24145
                     /gene="araD"
                     /locus_tag="ACOIAS_001179"
     CDS             23459..24145
                     /gene="araD"
                     /locus_tag="ACOIAS_001179"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGZ2700896.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            24163..24810
                     /locus_tag="ACOIAS_001180"
     CDS             24163..24810
                     /locus_tag="ACOIAS_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HGZ2700897.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(24983..26350)
                     /locus_tag="ACOIAS_001181"
     CDS             complement(24983..26350)
                     /locus_tag="ACOIAS_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGZ2700898.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(26540..26628)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            27056..29176
                     /locus_tag="ACOIAS_001182"
     CDS             27056..29176
                     /locus_tag="ACOIAS_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGZ2700899.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKTIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            29280..30533
                     /locus_tag="ACOIAS_001183"
     CDS             29280..30533
                     /locus_tag="ACOIAS_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HGZ2700900.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSKMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(30686..31747)
                     /gene="ytvI"
                     /locus_tag="ACOIAS_001184"
     CDS             complement(30686..31747)
                     /gene="ytvI"
                     /locus_tag="ACOIAS_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HGZ2700901.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            32021..32761
                     /locus_tag="ACOIAS_001185"
     CDS             32021..32761
                     /locus_tag="ACOIAS_001185"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HGZ2700902.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            32773..34551
                     /locus_tag="ACOIAS_001186"
     CDS             32773..34551
                     /locus_tag="ACOIAS_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2700903.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            34718..35635
                     /gene="pfkB"
                     /locus_tag="ACOIAS_001187"
     CDS             34718..35635
                     /gene="pfkB"
                     /locus_tag="ACOIAS_001187"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGZ2700904.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            35672..37591
                     /locus_tag="ACOIAS_001188"
     CDS             35672..37591
                     /locus_tag="ACOIAS_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGZ2700905.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT        15090 a         4086 c         6206 g        12382 t
ORIGIN      
        1 tttaatagaa tatgtatagt atatagtaga tagaatgtat aaaaataaaa actgttcaat
       61 aagaattaat atacattaat tcagctgaat ccaatcaaaa tacttaaata aaagtaatct
      121 gaaaataaca taattcaaat caatttattc aaataaacct caatttatct caaatcttaa
      181 gaagtcatta aacaaacttg ctaaattaca aagagaatta tcaagaaaaa caatcggtag
      241 tttaaataga aataaagcaa gattaaaagt tgcaagactt caagaacata tagctaatca
      301 aagaaatgat tttctacaaa aactatctac taaattaata aaagagaacg atattatttg
      361 catagaagat ttacaagtaa agaatatgat taggaatcgt aaactatctc gctcaatttc
      421 tgatgtgtca tggtcagagt ttattcgtca attagaatac aaggctaatt ggcatggtag
      481 acaaattgta aaggtgggta aattctttgc aagttcacaa atatgcaata aatgtgggta
      541 taaaaacgag gaagtgaagg acttaaatat aagagaatgg atttatccta gttgtaatga
      601 aactcacgat agagatataa atgcaagtat aaatatatta aaagaaggat taagactaat
      661 aacaattcaa aataaataac gtatgtaaga accgtaggaa ctacggggat agcctgtgga
      721 gaattagtaa gacatatgtt agtatgcaaa attctgtgaa gcaggaaacc tgtgacttta
      781 gtcatgggag gttcaggcta gtattactca ttattgtatt caaaagctaa agtggaaacc
      841 tagtgaatat atgaatttag aagttaatga gagagcatta gcagccgact caatacttat
      901 taaaattgaa gatgaagaag aaacaatgaa agaagctgaa agagagaaaa agagaggtag
      961 aagaaggtag aatttaaagt taaaatagtt acattgctaa tatatgaaaa tgttacaaat
     1021 ttggaaatag atggatatat atgttgtata gtataatgta gataatatat caccaagtaa
     1081 aaaacaacaa ataaatctaa attatgaggg gtggaaaata aagatattaa acttacaata
     1141 tgtatatttg cttttgtaat tggaataata gtgttatttt gctgtatagg tctaggtcaa
     1201 tttaatatgg attgtattat aaaacaaaat ggtgggtcaa tagctaccga acaatacaac
     1261 atatatttag aacaatctat ttcacaatat agaaattttg gttctatact ggttttacta
     1321 ggaggaatag gaatactgtt tgataaaagt gtcagaaaat actaaaaaag tatattttta
     1381 tttaggatgt gatgaatgat atggcaagag atgtatttag tgtaaagatg acaccactag
     1441 aagcacagaa aatggtagaa aaaaatttgg aagcagattt agtttctgtt gatatttata
     1501 acctagaaaa taataagtct ataatagtta caatctttga aaaatattat gcaagaagca
     1561 atagtgatgc aggtttggta gtgatttgtg aaaatactac aggaaaaaca cttgttaaaa
     1621 ttacctcaac tggtagtgca attggactat ttcagattga ttggggaaca ggtaataatt
     1681 tgattaaaag agtaaaaaaa atatttattg attatattat ttaattattt tatgtagata
     1741 ttcttaggtt ggtatggaat ttgtactaac ctattaagta atcaataaaa ctaaataaac
     1801 atagaaagca cttacaaata tgtaggtgct ttttattgaa aaaatagaaa ataaaataaa
     1861 aagtaagata taggaaaaat atgtaagaat tatatgttat aataattgta gcaagaagat
     1921 gtaatctaca atttatagag tggagttcat gtattaaaaa attatcctcc caacgataag
     1981 aagggaggtg aatatacatg gataattttt tatttaatgt gttagctagt gtaacagcta
     2041 gttacatagt ttatttgatt agcaagttaa tcaaaaaagt aaaaagccac tctaccgcaa
     2101 agagtgactt aaagattgat ataaatttta aatatcatcg taaaaaataa ctttcagtta
     2161 tagaacttca ctctaccgca aatagattgt agttcttctt gtttttatta taccacaaat
     2221 tagaaaaaat attgcatata atatttttta tagtcaataa aaatatgaaa ttttttatac
     2281 acaatgatga tgaatctaac tagatgaata aatttaattg taaataagaa agtacttact
     2341 taaatggtag gtgcttttgt tttgccaaat tatgctataa tattgagtga gatgagtaat
     2401 ttgtggggga attttataat gtggatatta gtaccaataa taacaatagt attacttata
     2461 atagctgttt cttctatgca atatatttta gttatgatag cttttttgtt gattatatat
     2521 tcttttatag aaaagaaaat agttatgggt cttgtgtcag ttcttttttt cacttattca
     2581 atttatcttt gtgcaacttg ggaagataaa tccttgatag cagataataa agttgagact
     2641 gttaaggcac aaagagaaac tgtagaaaga gaaaaagaaa tggaaagaag aagaatacaa
     2701 gaagaagtag acaaagaaag atatattgaa aaacatggaa tggaaatatc agaaaatgat
     2761 ttaaaagtta agctagaggc tttagtacct caagaatata aggggaagaa atatgaatta
     2821 aaagtaggaa aatttaaaag atatagtatg tattttgatt taactgtaca aaatgaaaaa
     2881 tttagtaatt ctgaagaatg taaaaaattt gtaaaagaaa ttgctaatga tcttaaaaaa
     2941 attaaaataa gcaaggcgta ctttaagttt cattcaaaag atgacggcgg tatatataat
     3001 tctgtttata ttgattattt tagaaacata caaaataatg tagataatgt tgaaaattta
     3061 gaattcaatg agtttgaact taaaaccgaa gaagaagaga agcgagaaca agagaaaata
     3121 gaacaagaaa aaaacagtta taataactac attcaaaaca gagtggttga cccactagat
     3181 agaataaaga aactaaaaga gttgctagat tcaggagcaa ttacacagga agaatataat
     3241 aaaaagaaaa aagaattatt agaatagata atatagtaag cacttacaag tatgtaggtg
     3301 cttttaaatt tacaaagtat tccattttaa ttttatagtt tagattttat gatataataa
     3361 aaatatagaa gttttgcagt gtgcgatatt tgttacaaag tagggcttaa tacttgaaat
     3421 ctaagatgtt gagggtgcgt gataagtgtt atcaattgca ctattgcccg ctcactgcaa
     3481 ttttaagagt attgtatata tgtaggtatt ggaaatgcta agtttatttt ggggttttag
     3541 attaactata tggaatgtaa aactgactcg ggagcatata ataagtaata tcaagaatag
     3601 ttttagatta actatatgga atgtaaaatt cgtaaaataa caattgtcag gaatatgaga
     3661 agtttgtttt agattaacta tatggaatgt aaaattggac tcaaaatata agttaaaaaa
     3721 atttttaagt tttagattaa ctatatggaa tgtaaaaatt tttgacgcta tgaagaaatt
     3781 tactacaaaa tttgttttag attaactata tggaatgtaa aaactttttt catcctccca
     3841 aacctcttgt tcgctgtcgt tttagattaa ctatatggaa tgtaaaaaag aaaataagtt
     3901 taaaaaaagg tttcattatt atatcgtttt agattaacta tatggaatgt aaaaccaatt
     3961 atgtacagtt tagttactgt gttaagtctt gttttagatt aactatatgg aatgtaaaat
     4021 ccttttataa atatcttctg acatgtgtat atgcttgttt tagattaact atatggaatg
     4081 taaatcggct acaacggtat aaattaaatt tagcagggta cattttagat taactatatg
     4141 gaatgtaaat tgctgtgatt tcaataataa aacaattaat aattgtgttt tagattaact
     4201 atgtggattc aaaattaaat aaacaaagaa agcacttaca aatatgtagg tgcttttatt
     4261 ctgctcaaaa ttggtcggtt gggtaaaata attagaaaaa gttagtaaaa acctattgac
     4321 tgtaactcgt tacaatattg ttattaatgt aacgagttac agaaaagagg tgaataaaat
     4381 agcaactaaa agtagggcag agtatatgaa aaatcgtcga aaagataaaa gaggttttag
     4441 tgtactttta gacaaagaaa agttagataa atttgatgaa gtgttagagg aaaagaatct
     4501 aaccaagaaa gaatggctag aagaaaaaat cgacgaggaa ctggaacaaa aggaataaaa
     4561 aataagggtc actcccaccg accaaagttt gagtaacccc tatgacgtat actatcgtat
     4621 atcaattata gtatatgtca ttccttaaaa aaatcaatta ttaaggagtg taatattatg
     4681 aaaaatgaat taatgatgtt tgaaggaaaa gagattgaag tatttgaatt tgaaggaaga
     4741 attttattca actcaaaaga tgtagtaaac tgtttagaca taaaaaatgt taatgagaat
     4801 ataagactta tgaatgaaaa gcaagttgta aagttaagaa attcggatat cagtaatact
     4861 gacatccgaa aacttaataa tgctggagaa aattttctta ctgaaagtgg agtgtataaa
     4921 ttaatcttta aatcaagaaa agaagaagct gagagattcc aaggctggat aagtgatgaa
     4981 gttttaccaa gcataagaca aactggtgca tacataaata ataatgcaat accgaaaagt
     5041 tgagagaaaa agcaagtgag attgaaaaat tacagttggt ttataacagt acatctatgt
     5101 taaaagaatt gctagatgat gcaggctttg acaataaatc aaaactatta acagctaaaa
     5161 cattatataa gaaagcagga attgatttac caatagagat aaacgaagaa gaacattatt
     5221 ttgatacaaa acaaatagca tctaaactga aaatatattc taagagtaat aaaccagctc
     5281 agatggcagt ttgtgagatt attaaaaaga ttgatttaga agatggtgaa gttaaaggtg
     5341 tttgggaaat taatggttct tggactggta ctgtaaataa atatacaaag agtgtaatag
     5401 ataaggtgag aacttggata gaggaaaata atagacctac caagattgca ggtgagaaga
     5461 agaattatta tgtattttac aaaattgagt aaatttatca gttgtattaa ataatttagt
     5521 ttagttttgg ggggattaat acaatgtgtg agaatttact tgataatatg tatattgaaa
     5581 agagaaaaga agaatataga attaaacttt tgaaaataag agaaacagat atagatatat
     5641 ataataagtt agaaagtata gtatataaac tttctgagaa aaaattagag aaaaataata
     5701 aatagacata taaagcactt ggatattcta ctgtttcaag tgctttgttt ggtataaaat
     5761 ggtataatgg agatagagaa ttatattaac ccaaaaatgg agaaaataga gaagatgatt
     5821 tagttgacgc tttatcagta ggagtacttg caggtaagac agaatctcta gtactattag
     5881 ttggaaatgg aataagtgat actcaaaaaa gtttaataaa taaaagagaa tttagaaata
     5941 taactcaaat tggtggaaat ggaaatgaga aagcatttag tgaagtagaa aacttagtta
     6001 aatagattag aaggtagcaa ctagagttgg ttgttacctt ctttttttat actttctctc
     6061 tctaagtatt ttctttttca tatcttctaa tacatctaat gcagtagtat aaaattgtat
     6121 attatttcta taaggtatag ctattggaag aatgcccttg ttaactaaaa aaataagaag
     6181 tacaataaat aaaagtgtca taatcattct aataaaataa ttagtgatat ctgataaatc
     6241 aaatgttaag ttaattgttg tagaaaatac aacagccata atagatatat acaaagcaaa
     6301 aatggggtta ctattttttt gtaactgtcc tttaagccta gattgctcta tttctatatc
     6361 ttgaatactt tttatggaat ccatttcact ataatactta taaactttta aataatattt
     6421 tttatacttg tttaaagctt cttcattatc tttaaggtct ttgtcactta actctgattt
     6481 aacttcccca aaatccataa aatccctcct acaatgttat ataatatact ttaatttata
     6541 gcatatttaa gaacttatat cagcaatttg tcgaacgatt ttagctaaaa atagagtata
     6601 atgataggct tattatttat aagatattat tttttatata gttatatatt tctttctaat
     6661 tagataatat aatgctgttg ttataatggc tactaatatt attgctatta ttgtaatatc
     6721 taatcttctt gtaaataagc ctattgctat atactcaaga cctgttacat agcgataaag
     6781 tacttctagt ttaacagttt ttttcttatc tatatttcca tatattgatt ctaatttatt
     6841 aaaatatttt ttactacaaa aagcagtttt aggtttaatt aaatatatta ttgtagttcc
     6901 tattataact attattccaa taagtataag attgatattt ccaaaatcca tagcagatac
     6961 ctcctattaa atttgctttt cttaatcaaa gtatatcagt aacacaagta caaagagcat
     7021 ctaaaatatt acaaatatta acaggataga taaaactata gtaaaaattg taataataaa
     7081 gcgaacgaaa attatcatac taaaacataa gacatgttat aattgtatta gatagaagat
     7141 taacatacac caaagcactc cttacaataa agagtgctta tttttttgaa attcatcaat
     7201 atataaacta tcaagaacct tattcaacac accttaaaat cgatttaaat agctcttttt
     7261 gataacataa tttatatgat ataataaaaa aatagaagtg taccaccaat acacttctat
     7321 agctataact aagcattctc ttgctggggg agtgcttttt tcatttctcc aaacaacatt
     7381 atttacttca ttagtaatta tcaaatcaat ctcattatct ttcaaaatct ctgttgcagt
     7441 acaaattaga ttatctaagt caaaacttcg agttatctct agttgtttgt tatttacttc
     7501 tctttttagt atataagttt taaaatacat ttatatcacc ccttgtattt actctttttt
     7561 ctctaaaaat atattgataa gtaaatatgc aacaaataaa aaaatctcaa tgacacatag
     7621 tgttatagct ataactagaa gtgattgatt tttccatgca ctataagcta tgatgaaaag
     7681 aaatgtaaaa gtaaataatg tataattcat aattttaact aagatatgac ttgttttcat
     7741 tgttaagtac aagtctcttt catctgcact atcgtgtaat tcttcaacaa tgccttttct
     7801 tgaaaaagca ttacaaaaat taaagattgc tattacaatt aaaagaaatg atgaaattag
     7861 gaatctttta tcttttaaaa tgcaataaat aactaaacta gcacaacata acatacaaac
     7921 tataataccc actataaaac ttcgtttact ctttactttc atgtttttta tcctccattt
     7981 ctttattttc ttttaaacaa catagttctt caatcgttgt accaaacact tgtgccatac
     8041 gataggcaag cataagagaa gggctatatt gctctttttc aatagaaata attgttctgt
     8101 ttgatacatg gactaaatca gcgagttgtt gctgtgttag acccatattt gttcgaaatt
     8161 ctcgtacttt tgttttcatt ccataacctc tttctatatg aagctaactt catgtgaagt
     8221 tagcttcata atatcctaaa atgagtaaaa tgtcaatctc attagataag tattataaaa
     8281 cctaaaagaa taatatggta aaaattggat ggaattattt accttaattc tcttgtttgt
     8341 aaaactttat tgatatagaa tttaacatat acataaatcg gcaaaggggc gcgaattatg
     8401 aaaattagta aattaccaga agcggaatta aaagttatga gatatatttg tgaagctaac
     8461 aaggtattaa cttctaggga attagttgct gcaatggaag aaaaatatga gtggaatgaa
     8521 tcaactacat ttacagtttt gaaaagatta gagcgaaggg agtttttaag tacagagaaa
     8581 ataggcaaac ttacacatta taatatacta gtaaaagaga aaaaatatct aaggtttgaa
     8641 acaaaggaat ttttgaagaa catacataaa aattcaattt caagtttaat gtcagcgtta
     8701 catggtgaag acgacgaagt ggatgaagat aaattaagtg aactcgaaga atattttaag
     8761 aatttgaaat aagaaaatga caaattgata cataataaat aaaatgaagg tatgaaatta
     8821 taactttttc taccttcatt ttgtttatta ttctgaacat gttaagaaaa tgaaataagt
     8881 ttgtatttta gttatataaa ttttaataga atatgtacag tatatagtag atagaatgta
     8941 taaaaataga atttaaataa gtagctatta ttgcagtgaa atgaatgttt ctaagtatat
     9001 ttgggtggaa gaatggtgga agaaaattag tgaaatgtaa ttcttccacc attcttccac
     9061 ccaaaaaata aaaactgttc aataagaatt aatatacatc aattcagctg aatgcaatca
     9121 aaatacttaa atgaaagtaa tctgaaaaca acataattca aatcaattta ttcaaataaa
     9181 cctcaattta tcttaaatct taagaaatat ttaagaatta ggataatatt ttcttaagat
     9241 ttatatttta ttatatagtt ataagggaaa gttacaaaaa tgtaagccaa agtaatataa
     9301 caatttagtc attgttgaga aagggagtga aagatattgt ataatataga taagtattat
     9361 tatacaatag aggaagtgaa aatatgtaca atatattagt agtagacgat gataaagaaa
     9421 ttgttgattc gatagaaata tatcttaaat cagaaggatt taaaatatat aaagcatatg
     9481 atggaatgaa agctttggaa ataataacag agaatgacat tcatctaata ctaatggata
     9541 taatgatgcc taaattagat ggaatcaaag caactgtaaa aatacgagaa gaaagaaata
     9601 ttccaataat tttaatttca gcaaaaagtg aagatacaga taaaataata ggattaaata
     9661 ttggagctga tgattatata acaaagccat ttaatctatt agagcttata gctagggtta
     9721 aatcaaactt aagacgatat gtaactttag gtaattatga agatacatgt aaagaaattc
     9781 taaaaagtgg agctttggag ttaaatacac taacaaaaga agttaaaatt gatggtgaaa
     9841 acataaaaat gactccaatt gagtataaaa taattgaact tcttttagaa aataaaggac
     9901 gagttttctc aatagatgag atatacgaaa aagtatggaa tgaggaaagt tttaattcag
     9961 aaaatacagt agcagtacat atcagacgaa taagagaaaa gattgaaata aatccaaaag
    10021 aaccaagatt tttaaaggtg gtgtggggag ttggttataa gattgaaaaa ttttagtgga
    10081 aaaattataa aagaaaagag tttaatattt aattgcattt tgattttagt catttttaca
    10141 tttacacaaa tgttaggaat tttaatatgt tttaattttt tctctgagag tttaatttac
    10201 tctatgggga ttatgctttt cttaaacttt attttatata aaatactgac aatgtagatg
    10261 aggtgggcaa cttggagaaa tatagagtat taaagttagt ttcattaatc ataatatcta
    10321 tctcatttcc attaactata ttgttggtga caataggact tgctgagcct aaatacaata
    10381 tacaaaaaac ttctacaaag agtgggattt ttgatatatc tgatgatttt tatgatagta
    10441 atatatttga cgaaggaaca atggcaagtc tattacccaa gtttaagcta tctgtaggtg
    10501 gtaaattaac tagtaaagaa aaaggtaaat tccaacaaga aggtaaaagt atagatttaa
    10561 gtagtagtga ttctgagtat gataaaatca tgattaatga acaagcttct gaagaatatt
    10621 taagttatct aaaaaatata aaagtgtatg ttattaatac atacacagga gtattttata
    10681 caaatacgtc atatgctagt ttatatgatt ttaaacaaaa aactaagagc tattgtaatg
    10741 tagagataat aaataagata ggaaagagct catatattaa aacaataaat ggagaaaaat
    10801 ttgaactgaa taatctgagt ctggaactaa atgaagtaga tgaaggtttt gaggcatatg
    10861 tatcttttcc agaagagcca actatagaag atggaatagt atatactaat tttcaaatat
    10921 ttaaacaagc tacagaaaaa gtaagattat tttttgtagc tagtttaagt ttagcaatta
    10981 tatgtttatt atctataatt ttatatatca aaataaaagg tgaagtttta aaaagaaata
    11041 atgtattttt actaatatac agtaagatac ctatggaatt taatgtaata ttagtatcta
    11101 tttcaattgc aattttaact atatatgctc ttgctgtatc ttatcatatt gatgtaattg
    11161 cattagtttc tttatcttta ctgatagtat caaataatat attttttata aatgtgcaat
    11221 tgagctattt agaaaataaa atgtgtatat ttaagacaag tattatagta agagtgattt
    11281 tatggttaat aaaaactgta agggaggtca gtaacgctat aaagaaagta tctttagcaa
    11341 ggaaagttat tgttatagtg ataatatctt tagctttaat catggcaatt agcactcgaa
    11401 gtataggtat gggattactg ttttctatat gtattttttt aggatttaca atatatataa
    11461 ctaaaagact ttcatatctt agttacgtaa tagaaggtac agaaagaata aaaaagggag
    11521 aattagatta taagataaaa attactggga atgataattt tacgtcttta gctgaaaata
    11581 taaataatat tggagaaggt cttgatagag ctatagaaga gcaagtaaaa agtgaaagaa
    11641 tgaagtctga ccttataaca aatgtatctc atgatttgaa aactccactt acttctataa
    11701 taaattatgt agatttaatt aaaaaagaag aatcaataca accagagtat ataaatgatt
    11761 atataaatgt acttgagtca aagtcaaaaa gactaaaatt attgatagaa gatttatttg
    11821 aagcgagtag agtaagtagt ggcaatatag aattacaaat tagcaagatt gatttagtgc
    11881 aactattaag acaaagtata ggtgaacttg aagaaaaatt atctaaaaat aatttatatt
    11941 taaaattaaa tgttccaaat gataaggtgt atatatgggc agatggaaga agaatgtata
    12001 gagtatttga aaatctttta tctaatatag caaaatattc cttagaagaa actagagtct
    12061 atattgatgt atatgactat ggtgaaaatg taaaggtaac tatgaaaaat atatcttctt
    12121 atgaattaaa ttttgaccca agtgagatta tggaaagatt taaaagagct gatgaatcaa
    12181 ggaatactga aggaagtgga ttagggcttg ctattgcaag agatttagta gctttacaag
    12241 gtggaaaatt ttatatagaa atagatggag atttatttaa agcaaattta gagtttcaaa
    12301 aatatgaaga agaaataaca aatatggaat gagatagtat ttaagtatat atattttttt
    12361 tactgtcgaa agcttttatt ataatatatt agaatattta cagtagagaa tgaagagtaa
    12421 aaacagagta actttaagtc actctgtttt ttatttaaaa tcaataatat aacaacttaa
    12481 attaaaattg tacacatagt tttaaaataa cttatacaat aaaacattat ccaaatatca
    12541 caagataatt ccaaataaat atatagatat cactcctgta ataatagaaa taccaataga
    12601 tttaaactta tatgcaacaa gaagtacaat cataaaagat ataattttag ggttagctat
    12661 agataaatat agactatctc cattaaaaaa tacatcttta gcagttaagg aagtaagtat
    12721 tgctacaggt ataaatttca tgattttttt caaaaaatca ggcatctctt tttttgaaaa
    12781 aattaacata ggaagcactc ttggtagata tgtaacaatg tacattccaa ttataataaa
    12841 aaatatataa ttattattca taagattcct cctttttaca gtaagattct tctataaaga
    12901 aaccaattaa agatgctaaa attgatgcaa ctattattac taaattgctt ttgaataatg
    12961 catataaaat aacagaaata atgattgatg atataaatac tacaatataa atcttatttt
    13021 ttatttgaga aactattaaa gctataaaca tagaagtcaa tatgaagcca gaaatagaac
    13081 tgtctataga tatactttct cctaaaaatg ctccaataaa attagctaaa actgatgaaa
    13141 ttaagcagaa gaaattaaga taaatagcat ttctatcaga ccaatttcca gcagtaaatt
    13201 tttcataatt aactgcaaaa gtttcatctg ttatagtatg acaaaatggc aatataaatt
    13261 ttggactttt attttttatg tacatgttca aattagaact cattagaaaa tgtcttaagt
    13321 taactataaa tgtagtcaaa acaatagatg ttacacttgc accagtagaa atcattgaag
    13381 ctgcaatgaa ttgagaactt cctccaaata ctaaagttac cattagtgct atttgaaaag
    13441 gtgaaagacc agatttttgt aatagtattc ctcctactat acctataggt atataaccta
    13501 agaatatagg aattgaagca ataaagcttt cttttaattg ttgagttctt tccacaataa
    13561 atcactcctt ttgtattaaa tgtattaaag tattagtaaa acagaaaaat agattaattg
    13621 tataattgca attataatta aaaataaatt tggattaata tataaaatac tatacaacaa
    13681 atagataatc ttaaacggtt attaaatttt atctatttgt tattatgatg taataataca
    13741 tattaatctc tataagttaa ttactatctg gtgtaatttt attttaacaa caaaaaaaaa
    13801 tacacacaat taaaatattg tgtgcaatac aattattttt tctcttctaa tagtcttttt
    13861 aatacgctca aaccagcttt aagttcttct gtactttctg tagcacatac agctattctt
    13921 attgcagaaa ttggtttttc ttttccaaca gcaaaacgtt cagaagcata gacttgtaca
    13981 ccttctttaa gtgcttgtat ctcaaatttt tctgccatta ttccatcagg tagaatcaac
    14041 catctaaata tacattcttc atttcccaaa agattatatc cagataaata ttgattaata
    14101 atcttgtttc ttcttcttgc agatttacgg cgtgcattaa ttaatttatc ggcttcacct
    14161 gatgtaatca tgcgataagc aatttctgtt aaaaaataag attgagatag attaatgttg
    14221 tataatgcgc ttgataatgc ctctcgatac tgtttaggtg tagagatata tgctagccgt
    14281 aaactaggtg caataatttt tgataaactc gtaatataaa ttgtttgatt tggcagatag
    14341 gaagccactg gatttaaatg tgtttcatta agtaaactat gaatagcatc ctcaataaca
    14401 attaaattgt atttagatgc aatatttgca atcatttttc gtccattttg tgacattata
    14461 tgtgtagttg gattttgata atctggtatt atgtatagac ctttaatatt ttcattttta
    14521 caagcatata atagtccttc ttcactaatc tcattatctt cttgtttaat tggaattagt
    14581 tgaatcccaa gcattttagc tgctgtcttt attccaggat aagttaatgg gtctacacct
    14641 attctatctc catgttgaaa caaaccagct aaaatagcaa caattgcatt ttgtccacca
    14701 cttgcaggaa gtaagctatc tgatgtagtt tcaagcccag ctttataaat taatttggca
    14761 atagcttctt tttgccattt agcaccatct ctttgtccat attggaatat agttttaaaa
    14821 tctatttcct taattatgtg tttaaacaaa gtattcattt cttctagcgt gaaatctgga
    14881 ttcattgtgc ccatttcaat aaatgtaatt ttattattac tggacatcaa gaatagattt
    14941 gaacgtgtat cataagatac aaaagttcca cttcctgtaa ctccacttat tagacctttt
    15001 ttttcacaaa ttttaaaagc tctagagata gtactcacat taacatctaa gaaatctgcc
    15061 aattcacgtt ggggtggtaa tttagtacct ggtaataaaa atccattgtt gatatcttgt
    15121 tctagttgtt ctacaagcgc cttatataat atttgccctt ttgaaggtct ctttggtttc
    15181 cagttcatag gataattttc aaaagaatta attggcataa gttttcacct cacattatta
    15241 atttgatttt ttctttatct aaaacaataa attctaaaga tacaaataaa aactgatagg
    15301 aaataattct atcagttttt gtcttttaat tgatttcaaa tcttcctata atttttatac
    15361 cttctttatc ataaagaggg attgtttttt taaatatatt aaaatttctt tttcttgtat
    15421 ataaaacagc ttcttctggt gtctttggtt ggttatcaca atcgtacaaa tttctttttt
    15481 ttaactaaaa gcaataatac ataaaattat tattgctaat gatactctta atgtggtata
    15541 tagtcatagt gtcacatgtc tatcaatagt tttttcagta attattgtaa cgaatagtag
    15601 gactcataaa atacttagaa atatgttttt agataaattt taaatatata atctaactta
    15661 tatctataaa ttaacactat tattctccaa ttccatttct agttttttta taaatttatt
    15721 aaattcttct ggttgccttt ttccaaacaa tactcttaca agagctgaat tgatttttcc
    15781 aaatatattc ctcttgctct cactttcaat aaaaataagt tttgtatttt ctaagtcaat
    15841 atgtactaat tcatatctag taatagtact ttctctttta ttgcttgttt ttatctcata
    15901 tactctattt ttttcgaaat tagttatttc aaccttaaat cttttattat ttttttgctg
    15961 tacaaatgtt cctactgctt ttttctcatt aaaattagga aaatttagtc ttgcagattt
    16021 tgtaaatata ttaaatatgt cttttgcaga atattttaat aaaacttcag ttttaaatgt
    16081 attcatatcg ttttcccttc tgtatataaa tttaatattt ataagttaat ttttaagtta
    16141 aaatagtata atatgccata ttaaatcatg ttaaaactat ttatatagct aaaatcttac
    16201 ctatataagt atgtatacta tcatataaaa ataaaatctt aaaatagaaa gggttaaaaa
    16261 cacagtttat tataattttt attgttgagt agatacgtac tattatatat gattgagcaa
    16321 gtttttcaat cataaattta taaagtattt tgcattaaaa tttaaacaaa gaattaaatt
    16381 atttaagatt atgaataact aatacattta gtaaattact aaatatacaa aatgtaaata
    16441 cttatcaaaa tgtataaata cttatgacat ctagttactt acagggttta agtagaaaaa
    16501 aatatcaaag taagtatggc atgatagttg cttatatatt tatcaagaaa ggagttatta
    16561 agcatggagt tgagagataa acttgtaaac atcataaata cagaaaataa aaaagatcca
    16621 ttaacagatg ttcaaatagc aaagttttta agtacaacta gagagaacat aactaatttg
    16681 agaaaagaac ttaatataag taattcaagg caaagaagat atccttattt aaaatcagca
    16741 atttcggcaa tattacaaaa gaataagaat atttctgtat cagaaattac aagggaattg
    16801 atgacagaag gctttaatat atctagaaga gttgtggagg aacttttacc taaagaaagt
    16861 ttagaaatgg atgtagtaga ggaatctgaa gaagaatcga atgacccatt tgaaactctg
    16921 ataggtaata aagggagtct tagaaatgct gtagagcagg ctaaatctgc tatattgtat
    16981 ccaccaaaag ggttaccaac gttgattatt ggggaaagtg gagttggtaa gagtttattt
    17041 tctagacata tgtatgagtt tgcaaagcaa aaaaagatag taaaagaaag ctccaatttt
    17101 gttgtattta actgtgctga ttatagtgac aatccacaat tattattatc tttgttattt
    17161 ggatataaaa aaggtgcatt tacaggtgct gaatatgata caccaggtct tgtagaagaa
    17221 gctgatgaag gagttttatt tttggatgaa atacatagat tgcctccaaa aggccaagaa
    17281 atactatttt ctattttaga taggggaaag tttaggagat taggtgaaac taatgctgaa
    17341 agaaaagtaa gtattatgtt gattggggca actactgaaa atgtagagac taatttatta
    17401 ttaacattca gaagaaggat accaatgcta ataactttgc caccattaca tgatagatta
    17461 ctaaaagaga aaatagacct tatatacaat atctttcaac aagagtgtaa tagaataaat
    17521 gcaaaaatat ttgtagataa aaatgtgatt gaaatacttg cactaaaaaa gtttagtgga
    17581 aatataggac aattacaaaa tatgattcag gttctttgtg caagagcttt tatgaagttt
    17641 ataaattcca aagatgaaga tagtgaatcg atagtagtag tagatataaa tgaagtttta
    17701 aagcttaaag attcttttaa agatgctgcc tttcaagaga ttgaatatac agagataaga
    17761 aaatatttga aaaatgctat atttatacca tttaatttag aagaatcatc agtaaaagga
    17821 agtcttttaa gtgatgaata tatattacca gaggacatat acaaaacaat tgaaaagaaa
    17881 tattatgacc taaaatccct agacattgaa gatatagata tagagaatat tttatggact
    17941 tttattttaa atagatttgc tgacttaaag tttaatctcg atagtaatga agaaattttt
    18001 tcaatgagtg atttaaatag ttttgtaagt gatagtttag ttaaactggt aaaagaactt
    18061 atgaatgata tagttgaaaa aaatatgaat ttagaagtaa ataaaaatat gttcaaatat
    18121 ctagcaatac acttagaaga agctataaaa agaattaagc taaatcaaaa gataataaat
    18181 gtaaatttag aaaaaataaa gattgacttt tcaaaagaat atgaaattag taaaagtttt
    18241 gcaataaaaa ttgaggaatc aattggtatt aagattccag atgatgaaat tggatttata
    18301 acactctaca taaagtctgc tctaaaaaat gaagtgaaaa aagataaagt agggttaata
    18361 ataatttctc atggacgtat agctacagag actgtaaatg tagttaaaga acttttaggt
    18421 gtaaagtttc ctgtggctat agatatgcct ttagatgaaa aaccaataaa tatatacaat
    18481 aaggcagtag aactgtcaaa aataatagac caagggaaag gcatattgtt tttggtggat
    18541 attggttcac ttacaaacat aggtcaaata gtaaataaga gaaccaatat aagcactaaa
    18601 actattgata gagtagacct cctaatggca ctagaagcaa ctagaaaagt ttctatggga
    18661 gaggaagaac ttgatgaaat atttttttct ttgctaaaag ataggatggg atataactat
    18721 gaattagaaa aacacataga taaatcaaat gcaattgtaa cattatgtct tactggagaa
    18781 ggaactgcta agtatataag taaaacgttg gaagaaaagt atgataacac taaatgttat
    18841 caaatgagtg cattggatga aaatttattt tgtaaaattg aaaatctaaa agagacaaac
    18901 aatatattag ctattgttgg gacaataaac cctaagatac caggtataaa ttttataccc
    18961 tataataaag aacttttcaa aaatttggat atctatctat caaaatcaaa acaacaagat
    19021 gacagaccag ttaaatcgta tgaaagaatg cttgatgagg atttagtgat ttttgaacca
    19081 gatatatatt ttaaaaagga tttattagaa tatgtatgtt caattcttat aaataaagaa
    19141 tatgtagaaa aagattactt agattctgtt ttacatagag aagaaatgct tccaacatat
    19201 tctaaaggag ctataggtgt gcctcatgga gattcttcta ctgtaaatag tacaaggttt
    19261 gtttttgtta aactaaagaa tcctattgat tggggtgtag gtaatgtaaa tttcatattt
    19321 atgccagttt ttcaggctaa tgataaagag attgttaaaa atgttcttga gatactaaaa
    19381 gatgcagaat ttatgaacaa tgccaataag tgttttgata aagatgcttt tagaaggatt
    19441 atatttgata aatttaaaca tctgtagcca tagcaaaaaa attgagagga gttagagtaa
    19501 tgaatgtaga tttaatatca ttgggtttag aatctaatga ttatgaaagt gttattgaag
    19561 aattaggaag tataatgtgt aaaaaagaat atgtaaaaga aacatacata aatgctgtct
    19621 tggaaagaga aaggacatta ccaacaggtc tagatattgg agaaatgtgt gttgctattc
    19681 cacatacaga ttcaaagcat gtaaatgagt ctaatgtagc tgtagggata ttgaaaaatc
    19741 ctgtaaagtt taattcaatg atagacccaa aagatagact tgatgtagaa cttgtatttt
    19801 tattagcagt aaagaaccca gattcacaag taaaactgtt aaaggattta atgtcagttt
    19861 ttcaaaatat aaagttatta aaaaatataa aaaacgcatc aacaaaagaa gaagtagcta
    19921 aattattgga ttttatagaa atttaatgta ggagaatttt ataaataaaa aattaaaatt
    19981 taaacaatca aagtaagtat tattttttta ataggtttaa tcttatataa aataaaaatt
    20041 tagagtgaaa aattaggagg aaattattat gaaacaattt aatgtattat cagtttgtgg
    20101 ttcagggaca gttacatcat ctatggtagc aggtaaatta aaagaagtac taggtaataa
    20161 ggggatttct attactacaa cagaagctag accaacagaa gcactaaatt tagcacaatc
    20221 aggaagattt gactttataa catttacgag tccacttcaa acaggcgatt atggaatacc
    20281 aactataaat gcatttgctt gcctaactgg tataggggaa gaggcattct ttgaagaagt
    20341 tttgactgtt attgaaggga tagataagta agttttggag acgtgaattt atagtattta
    20401 aatcaatgat ttagatacta taataagcta atattaacta aatatttgtg gaaaaaacat
    20461 tttatatgtt gtcataaatt aaaaaattat aaaaatagtc acttaagtat taaattaagc
    20521 ttataaatat ttataagtag gagggaaaaa gatgttagaa tttatgaaat ctataatcga
    20581 tacatttggt tctgcaatta tagttccaat tataatattc ataatagcaa agatatttaa
    20641 agtaactact aagaaatcgt ttctttctgc tgtgtatgca ggagttgctt tacaaggttt
    20701 tacactgatt ttaaattcat ttacaccaat aataacacca gttatcaata ggatggttga
    20761 gagtacagga gtaaacctac cagtttttga tgttggttgg caagcaacat cattggttgc
    20821 attttcaaca agtgcaggta tgatatattt aggtcttggt atattattac aaacaatttt
    20881 atttttgata aaatggacag acgtgtttca accatcagat ttatggaata actattctta
    20941 tatggtatgg ggagcaatgg ttataggagt aacaggaaat tttcctttag gtatagcatg
    21001 tatggtttta ttaaacctat atagtttatt aatatcagag ttagttgcaa agagatggtc
    21061 tagttactat agatatccta attgtacaat aatagctatg cataatgttg aagcttctgt
    21121 gtttgcagta tttgcagacc ctatatacaa taaattggga ttaaataaaa taaaattaaa
    21181 tccaaaagaa ttagagaaga aattagggtt tttaggtgaa ccaattactt taggtctttt
    21241 tttaggtatg tttataggta tattaggtaa tatgacgaga ataaatacta tggaagcttg
    21301 gggagaaata atgaaagtag gtatatctac aagtgcagtg atggcaattt tccctaaagt
    21361 agcaagtatg tttgctcaag catttgcacc tataacagaa gcagctagaa agattatgca
    21421 aaaagctgga aaccgtgagt ggtacatagc agtaaatgat gctgttgggt atggtgaacc
    21481 tgcaacttta atatcaggtc ttatattaat tccaataatg cttgtaatag ctatggtact
    21541 tcctggtaat aaggtattac cagttgtgga tttattggca attccatata tggttcaagg
    21601 tttagttgcc atacataatg gtaatatacc aaaagtattg gtttcaggta taatttggtt
    21661 tggattagga ttatatgttt gtacatctac agcaccatta tttacagata tggctacaaa
    21721 tataggtgta gctatacctg caggagcaat gttaataaca agctttaata tactaggaaa
    21781 acctttgatg ggattagttt tctttgcatt tctaagtgca aatccaattt atataggtct
    21841 ttcagttgta atttactttg ttctatgggc attgtttaga aagaacaaaa caagtattct
    21901 tgattattta gaaaaacaag cacttaaaaa tgtagaagaa gagcctgtag cagtttaagt
    21961 ttataaaaat gaaataagaa taattaaagg agtttataat tgattttata taagtatcag
    22021 ataatacctg agaaatattt aaatgaaata tagacttctt tttatttgat aatatttgta
    22081 attataatgc attgtatctt tatagttgag ttttctgtat aacctgctat ttaaaacata
    22141 ctcttttata tttatacagt aatgtggttt aattttataa aatgtaatct ctactggtaa
    22201 aagactttgt atataaggca tttgcctgaa taaaccattt atcttttgta tggcatagat
    22261 attgctatat agaagtatat agcatgaaag gagatattta tatgggagat aaaatgagag
    22321 catcagtatt gtatagtgta ggtgatgtca gatatgaaat ggtagatata ccagaaataa
    22381 cagatacgca agtgcttgta aatgtaaaat atgttgggat atgtggttca gatttaccta
    22441 ggtctatggt aagtggtttg agtggaaata caaaatatcc attgatttta ggacatgaat
    22501 tttctggcga agttgttaaa attggagaaa aagtaaagca tattaatgta ggagatagag
    22561 tagctgttgc accattagta ccttgtggaa aatgtgatta ttgtaatgaa ggtaattttg
    22621 gcttgtgtga tgattacaat ataattggaa caagagtaaa tggagcgttt gcagaatatg
    22681 taagagttcc agaagaacat attttaaaac taccagatac attggattat gaaactgctg
    22741 ccggaattga accagcaact atagcttatc atgggatttc aaagtctaat attagagtag
    22801 gagatagtgt agttgtgtta ggatgtggtc caataggtca gtttgttata caatgggcaa
    22861 aggtgtttgg tgcatctaag attatagcag ttgatatttt tgatgaaaaa ttagaacttt
    22921 ctaaacttct aggagcaaac tatatactta actcaaaaga agtaaatgta atcaaagaaa
    22981 taaaaaaaat aacaaatggt ggtgctgatg ttgtaataga gactgctggt agccgattta
    23041 cacaagaaca atctctattt gtagcaaaaa agaggggaaa tatagtgttt gtaggtatat
    23101 cacacacaga attaccactt agtgcagacg ctactgagtg tatattacgt ggagaactta
    23161 ccttgaaagg ttcttggaat tcatatacaa gcccttatcc tggaagagca tggactgcaa
    23221 cattggattt tatgggaaaa ggtgacatta tttttaaacc catgatttca cataaaatag
    23281 gtcttaatga agtgggtgac tttttaagta aaatgagcaa gagagaaatt aatttcaata
    23341 aaatcctagt agaaatataa aaatttaata aaaaattcta aaagcaatta aattttataa
    23401 ctatgacatt aaataaaata aaaataattt tcttaaataa aatataggag gaagaaaagt
    23461 gttagaaaat ttaaaagaag aagtattaaa agcaaactta gaattaccta agaaaaagct
    23521 aattacttat acttggggaa atgtaagtgg aatagataga gaaaaaggat tagtggtgat
    23581 aaagccaagt ggtgtagagt atagtaatat gactgtagaa gatatggttg tagttgattt
    23641 agagggaaat atagttgatg gcaaattaag accttcttca gataccccga ctcatctagt
    23701 tttatataaa gagtttgaag aattgggggg aatagtacat acacattcaa gttgggcaac
    23761 aatttgggct caaataggaa aatctatacc atcttgtggt actactcatg cagattattt
    23821 ttatggaagt ataccatgta ccagaaaaat gactagagaa gaaatttgca atgaatacga
    23881 aaaggaaact ggaaatgtaa tcattgaaga atttatggga agaaatccaa ttcattgtcc
    23941 aggaataatt gtaaatgacc acggaccttt tacatgggga aaagatgcaa atgaggctgt
    24001 acacaatgca gtagttttag aagaagtcgc aaagatggct tattatacag agttaatgag
    24061 tcctgataat attatggata aggttcttat gaataagcat ttttcaagaa aacatggaaa
    24121 aaatgcatac tatggtcaaa aatgattatc tggaggtgaa ttatgctaga ctctttaaaa
    24181 ggtaaaattg tggaaatagc aattaaagca gagcattatg gtttatgtaa agaaaagact
    24241 ggtaatttta gtattagaga taaggatact ggatatatac ttataacccc atcaggtgta
    24301 ggaagagaag aattaaaagt tgaacatatt tgtgtattgg atttggatgg aaatgtaata
    24361 gaagttgaga aaggtattaa gccaagtagt gaggtattga tgcatattga aatatacaaa
    24421 acaagagaaa atataaatgc catatcacat actcattctt tatatgcaac agcattttca
    24481 gtagcaaaga aaccaattat accaattgta tatgaatcag ttaattatgg cggttatgta
    24541 gatattgctc catatgaaag accaggaact ttaaaattag ctaagagtgt ttcaaaatta
    24601 ctgatagata cagatgcatg cttattagaa agacatgggt tgacaactgt aggtaatgat
    24661 ttagacgaag cattattaaa atcaagatat gtggaagagg tagcagaaat atactatagg
    24721 tctttagtat taaatcaatt taaagaactt gaagtagtag atgttgatga gtttaaatct
    24781 tggaaatacc cagaagaaat aaagttttaa tctaaataat tttcattgaa gtggttaatt
    24841 aaaatatatg tattatttat atcaattact atgttttatt atgttaatta ccgtattttg
    24901 caaatatatt tattacattt tttcaaaaaa atatagaatc cgaaagccat ataaaattga
    24961 cttgggattc tatacttttt atttatgcat ttttctttat ctgattttct ggagaattta
    25021 tatgttcaaa atatttttta gtttcagata ctattatagg tgataatgca agtaaagcta
    25081 taaggttagg tattgccata agcccattta caatatcagc aattatccaa acaacttcta
    25141 actttaagaa agcacctaat aacaccatag ccacaaatac agttctatac ccattcatgc
    25201 cttttacacc aaacagaaat tcaaaacatc tttctccata ataactccat cccaaaattg
    25261 tagtaaatgc aaataatgtt aaactaactg taagtaatat tggtccaaat gtaggaattg
    25321 ctccgttaaa agctgcttgt gtcataacag ctccatttag gtctgaacac cacactcctg
    25381 aaataacaag agagaatcca gtaagagtac agattataat agtatctata aaagtacctg
    25441 tcatagatat aagaccttgt tccgctggcc attcagtttt agcagcagca gcagctattg
    25501 gagcactacc tagaccagac tcatttgaaa atacaccacg agcgatacca tttctaattg
    25561 ccagtgacat agttgctcct aaaaaaccac cagtagcagc agtaggtgta aatgcacttt
    25621 taattattaa cataaaagtt tcagggattt tattccaaaa agatattaaa atgattccac
    25681 agattacaac atatataact gccataaaag gtactatttt agtagctaca ttagatatac
    25741 ttttaagtcc tccaaataca accaaagcta caaatactgc taatacaacc cctgtaattc
    25801 tagggtctat cccgaaacta ttatttattg catctgttat tgaatttact tgagtaaaag
    25861 taccgattcc taaaagtgca actagtatac cacttatagc gaaaaatatt gcaagaggtc
    25921 tccatttttc tcccatacca tttactatgt aatacattgg cccaccagac acttggccat
    25981 ttttatcttt agttctgtat tttattgcaa gaacaccttc tgaatactta gtagccattc
    26041 caaagaatgc tgctatccac atccaaaaaa gtgcaccagg tccaccagct ttgatagctg
    26101 tagcaacacc aacaatatta ccagtaccaa ctgtagcagc tagagcagta cataaggctg
    26161 caaaacttga aacatcacca ctacctttgt tttcagcaga gaatattaac ttaagagctc
    26221 ttggcagttt agttatttga acaactccaa gcttaacagt aagtagtata ccagtaccaa
    26281 ctaatagtac cagtaggggt ggtccccaaa taaaagcatc aactttgttt aaaatatcaa
    26341 ttagattcat aaaaacactc cctcattttt tttgctttac ataatcaata gaatttttaa
    26401 tgaagtaatt gacaggaaat aagtataatt tattaataat ttctagtgta ataaaaaaag
    26461 ctaaatctaa aaaagattca gctctggatg tacactaatt agcaaccaaa ataaaagtat
    26521 tagtaaatac tgtttttggt agataatccc tgtccttttg cctgagagat tgagcataaa
    26581 agccttgctc cttcggcgcc cataatgggt ctctccagag ttccgtaaat tttacagtcc
    26641 tacttttaaa agtacctgag agtgttactc cttcggtgtg ctatatagca actcttctat
    26701 aaaatcttaa ccggatattg atttgtcata gcttattgta ttaataatat taatataagt
    26761 ttttatgttt tgtcaactaa aaaattaaaa aacaaagaaa aaatataaaa atgtaagcct
    26821 atttacgaat tataaactta gaaagatgag taatatacgt gaaaatgaat tttgtaatac
    26881 acatacaaat gaagtgtaaa ataagttcta aataaaaaaa taataaaatc ttaaataatt
    26941 aattagatat gattaaatta tggtaatatt atagttacga tattaagaaa agttctattt
    27001 agttagattt taagcgtaat atgagattat gtatagagaa aggatgtatt tttagatggg
    27061 aaaaatatta gtgctagcag aaaaaccttc tgttggaaga gatatagcaa aagttttagg
    27121 gtcaaaaaat gaaaaaaatg gttatataga ggggccaaag tatgtgataa cttgggcact
    27181 aggtcatttg gttacacttg ctgaccccga aagttatgga gaaagatata aatcatggag
    27241 cttagaagac ctgccaatac ttcccaagca ccttaagact gttgtaataa aaaagagtgg
    27301 aaagcagttt aatacagtta aatcgcaaat gaatagaaat gatatagatg aaatagttat
    27361 agcaacagat gcaggtcgtg aaggagaact tgtagcaagg tggataatag aaaaatcaca
    27421 ggtaaaaaaa actataaaac gtctttggat ttcttcaagt acagacaaag caataaaaga
    27481 cggatttgca aaattaagaa gtggaaaaga atatgaaaat ttgtattact cagcaatagc
    27541 acgagcagaa gctgattgga ttattggaat aaatgcaacc agagctttga caacaaagta
    27601 caatgcacag ctttcttgtg gaagagtaca aactcctact cttgcaatgt tgcttaaaag
    27661 agaagaagaa ataagaaatt tcaaacctaa agagtattat ggattagaat taattgctac
    27721 aaagggaaat tcagatataa aatttatttg gaatgataaa aacaataatt cttcaacttt
    27781 tagtaaagaa aagatagaat ccacattaaa aaaagtaaaa ggtgtagaca taaaaatatc
    27841 tgaagtaaac aagtcatata agaaaaaata ttcacctgca ctctatgatt taacagaatt
    27901 acaaagagat gcaaataaaa tgtttggatt ttcagcaaaa gaaactttat ctataatgca
    27961 aaaactgtat gaacatcata aagtacttac ttatcctaga actgattcta gatatttaac
    28021 atcagatatt gttggaacat taaaagatag aataaaagct gtaaatatta gtgattattc
    28081 aaagacttgt aataaattat taaaaagcaa tattatagct aataaatcgt ttgttgacaa
    28141 ttcaaaggta acagaccatc atgcaataat accaacagaa cagaaggtat tcatgagtga
    28201 ttttagtgac aaagagcgaa aaatatataa tcttgtagta aaaagatttt tagcagtact
    28261 atctcctcct tttgaatatg agcagacaac tataagtgca actgtagaag gagaaatatt
    28321 taaggctaag ggaaacaaag taataaattt aggttggaaa gaaagttata aagatattga
    28381 agatgaagat gagtatgaaa gtataccaaa tattttagaa aaagatgtac ttaaggtcaa
    28441 gggattaaag ataacttcag gaaaaacaaa tccaccacca tacttaaatg agggaacatt
    28501 attgactgct atggaaaaga atggtctagg gacagttgct acaagagcag atataataga
    28561 aaaattattc aattcatttt taatggaaaa gaaaaataaa gatatatata taacatcaaa
    28621 aggaagacaa ttattagatt tagttccagt agacttaaag acaccagaac ttacagcttc
    28681 atgggaaaag aaattattgg atatttcaaa gggaaaactt agtaaaaata cattcataaa
    28741 tgacatgaaa gattattcaa aagatattgt actagaagta aaaaatagtg ataataaatt
    28801 taaacatgat aacttaacta aaaatagatg tccagaatgt ggtaaattta tgttagaggt
    28861 aaatggtaaa aaaggtaaga tgttgatttg tgaagataga gagtgtaata ctagaaaaac
    28921 tatatcacaa gtaaccaatt caagatgtcc taactgccat aaaaaattag aacttcgtgg
    28981 agaaggtgag gggagaatct tcacttgtag ttgcggatac agagaaaagt tgtcgtcttt
    29041 caacaagaga aagcaagaag aaaaacagaa ggcatcaaaa aaagatgtta atcaatattt
    29101 aaaaaatcaa aataaaaatc aagagacatt taataatcca tttgcagaag cattagctaa
    29161 gctaaaaaac aaataatttt aaagttatta ttatacacaa ttataacttt aactgtatat
    29221 tagttatatg tactgatata atatagtgta tagtttaggc atgaaagtag ggctgttaaa
    29281 tggatttaag tggcatattt aaatactatt gtaaagagtg tgaaaataca tggaataatt
    29341 cgagtgttga attatttgag aatatagaaa cgtatagtaa agattcacaa aaaaagaggg
    29401 aaaaagaatt agataaattg ctaaatacaa tatcagttca tttagagagg tatccaagtg
    29461 atgctgtatt gagaaaaatg tgggtaaaaa agggcgaggt tttcttacaa aagacattgg
    29521 aaaaagaaaa tatttttaag ttagaaaaaa tggatgtaga ggatagaaaa aaatttttag
    29581 atataacaaa acagtttatt agagatgcta gaaaatttga tgatgattta cctataggtg
    29641 atattatgca agctatgagg aatgtatgga tttcaaatgc attacaatta ttatttggta
    29701 aagaagtata ttattcaaaa gctaactttg catatagtat gttatatcca tatactgata
    29761 attatttaga caatacaaat atagataaaa atgataagat tttatttaat aactggttag
    29821 aaaaaaggct cctgggagaa cacattaaat ctaaggatta tcatgaaagt aaagtatcta
    29881 aaatgataga ttatattgaa agtgtatacc ctagagaaaa gtttacagaa gtttatgaat
    29941 cgttattatt aatatttaaa agtcaagtaa atagtttaaa acaacatggt aaggaaaatc
    30001 atttgtgtaa agaagattta ttatccattt ctattgaaaa aggaggttca tccgttttag
    30061 tagatggata tttaataagt ggattgatga caaaggaaga aatagagttt tgtataggat
    30121 atggattttt attacaaata tctgatgatt tacaagatat aaaagaagat ttaaaataca
    30181 accataaaac tattattaca gagatgtcaa aagagggtac tttagataaa gttgtaaata
    30241 aactaataaa ttttactatt gagttaatag atagttttaa aattaataat aaaaataaat
    30301 ctgtaataac tatgataaag aatgattgct taatgttaat tttattttct gtagtttata
    30361 atgctgaatt tttttctgta ggatatataa aagaagtaga gaaatttatt ccatatacaa
    30421 tagattattc attagagatt gaagaaaaaa taaaagaaaa atttaaaaat atagatgttt
    30481 taaataatga aaatgaatat aaagaaatga ttgatattat ttgtgcagag tagaaaaaaa
    30541 gggagtgtct tagaatagac tttttagttt atgaggcact ctttttgtat aaaatcaaat
    30601 gtattttcat cattttaata attaaaaaga gcttatccct atttgagaaa gcccttttta
    30661 atttaataaa aatgtcaagg ttaaactatt taaatgcagt aataatccca ttatcatgta
    30721 agttttttat gattgtagct gcgataggga atccaaataa tccagctatt ccaaatagtt
    30781 gagctccaac aaacatacac attaatgtta ttaaaggata taggccaatt tggtgtccta
    30841 ctacctttgg ttctaatatt tgtctaacaa cggttataat tatgtatagt attaaaagtc
    30901 ctattgctaa attaatattt ccattaatga gtgagataat catccaaggt gtcaaaatac
    30961 ttcctgtacc caagactggc aaaatatcaa caagtgctat gatgacagat atagtaaatg
    31021 cattttcaac ctttaggata gtaagtccaa taagtagttc tatgaatgtc actgatagta
    31081 gtattgcata ggcttttagt agttttaaaa gagcattaat actgctattt tttatattta
    31141 ttatcataaa ttgacttttt tctggaaatt gttttaagat aaagtttact attttttgat
    31201 agtcaaaagt aaagaaaaaa gaagcaatta aagtaataat aagttttaac ataaatgcag
    31261 gtgttttact tgcaatacta gcaatagcat tgagtgcatt tgttgaaata gaagctacta
    31321 aagacatcat agattgactt atattatccc aaccaagtat taatgctagg ttaacattag
    31381 gtgtactagc atctatcttt gaaaataaag tatttagcgt tggttgtata cttgatttat
    31441 atatttcagg aagtctaaaa aatacatcac ttacacttgc aaatatcttc acaccaaagc
    31501 taactaataa aaatatacac aacccataaa aaattaaaag tattactata gaaataagtg
    31561 tacgatttaa atttgtatta tttgtaataa gtcttataat aggtcttaag atggatgcaa
    31621 tgataaaact gattacaaaa ggcattaata atggcaataa gaattttaat acaatatata
    31681 caatacttgt tattaaaact acatatgtaa atttaattat aaaattcttt tgcttttgaa
    31741 tatccataat ataaaacctc caatgtattt gatattattc taaatatggt cttttttaca
    31801 gaaaatatac tactgtatat tgattataaa ctataaagct tatgtaaagt aaataaatta
    31861 tgtaaaattt aggttaattt tgtataaaga gatactttta tattttacaa ttaataacca
    31921 aatactaaaa aatattttaa ataaagaact taaaattgtg ataaaattaa tttatagata
    31981 ttaaaaaaag tataatatat ggtttttaag aggtgctaag atgaaaaatt ttagagattt
    32041 aggtgggaac aaaactgagg atggcagaac tgtaaagaag ggattatttt ataggtctgc
    32101 aaaattatca aatctaagtg aaaatgatat aaagatttta aaagagttaa atattaaata
    32161 tatatttgat tatagaagtg atgaagaagc tcgtaaacat ccatcaacaa taatatcaaa
    32221 tataaaaaat ataagaatac cagcaatgag agagctagaa gaatcaggag gaagttttgg
    32281 ttctattgaa gatatgattg atggattgtt tgaaaaagat ggtgcattta atatgttaaa
    32341 taatagttac tataatttgc ctataaataa tccgtcatat aaaaaattag tagaacttat
    32401 aagagactat tctaatttac caatattaaa tcattgtaca gctggtaagg atagaactgg
    32461 tgtggggagt gctataattc ttatgatttt aggggtatcg agagagaata taatgaaaga
    32521 ttatctaaaa agtaatgatt ttgcagataa agagatagaa agatttatag aatataagcc
    32581 taaatttaag gacattccaa aagaaaattt gaaatatata tttggtgtaa atgaagagta
    32641 tatgaaaact gcatttagaa gaattgatga agaatacatt agtgtagaag catatttata
    32701 tggagaattt aatttaaata aagaagaaat aagaaaatta agaaatcaat atctagagta
    32761 aaaggtgagt ttatgagttt aaatataaca gcaagacaga taaatataat aaaaatattg
    32821 ttaaattcaa aagatacaat tagtggtatt gccttaagtc aagagatagg atgttcttca
    32881 aaaactatac aaaatgaaat aaaggatatt aataaacaac ttagaaatgg taaaattttg
    32941 tcaataagag gagttggtta taaactagaa ggaagttttg atgaggtaaa tctaaatagc
    33001 aatctctatg atgatgtaga tagagtagaa tatataatca gaaaaatatt aaccttaagt
    33061 aatgaagaaa aaaatactat aaaacttgaa gatttagccg attctatgta tgtaagtgtt
    33121 tcaacagtaa aaaatgattt aaaagaagta aaaagaatgt tggaaaagta ttcaatatca
    33181 gttaaatcta agcataaaca aggtatatgt gttttagaaa gtgaagataa gatattaaat
    33241 tgtatagttg acttaagcaa taagagagat aatcaattga gtttaaatga ttttttaaat
    33301 gatgatataa aggaaagaaa actattaata aaaaaattat tattagacat attaaatgaa
    33361 aatggattag tattaactga tatagaattt aagagtgtgt taaataatat attagttatg
    33421 ttaagcagac ataagtataa tgaaaaagaa tttatagaga attatataga aaattatagg
    33481 agcaaaagaa gctcaatttt agaaaatgaa caaaacaaag aaattataat aaattcaata
    33541 aaaacatttt gtaaaaattt aaaattagct acatcaatag atataagtaa tgataaggta
    33601 tttgaagagt ttttatataa gcatattagt agtttatgta aaaaaataaa gttaggtata
    33661 aatcaatcac ctataatggc tcaagatata aaaataaaat atccatttgc ttttgaacta
    33721 gctaatatag ctaaaaagac aatagaaaaa gatttgaaga tagaaattaa tgaagatgaa
    33781 gttgcaaaca tagctctaca tataggagga gcagttgaaa gagcatctta taataataaa
    33841 gataaggttt tcaaaactat aatagtttgt acttcaggca taggcacatc aatgttaata
    33901 aaagctaaat tagagaatat atttaaggaa aaattagaaa tactaaagat tattccttca
    33961 tacttagttg aatatataaa agttatagat gttgattttg taatatctac tgttcctatt
    34021 gaagtaggag atgtgccagt aataaatata tctccaattt taagtgagaa agaaattaga
    34081 ttaattgaaa aatatataga aacaggaaat atatacctag atttagatat aaaaagcctg
    34141 tttgatagtg aattgttttt tactgatttg gtgtttaaaa ggaaagaaga agttatagat
    34201 tttatggcaa gtaaacttgt acaaaagggc tatatagatg aggatatgaa aaaatcttac
    34261 tttgagagag aaaaaatagc aacaacagaa atagggaata tggtaactat accacatgga
    34321 gctattggaa aaatttttga taataaaata gcaattggta tattaaagaa gcctatttct
    34381 tgggaaatat cagatgttcg tttaataata atgttagccc ttgataaaga taaaatatta
    34441 gattatgaag taatattttc aaaaatatat aaaagagtag attctatagc aaaagttata
    34501 agtatatgtg aaaatagtag ttttgaaaaa tttgtaaatt tatttaatta gatgggtagt
    34561 attgtatata cacctgtaaa tttggataat ataaaactaa ataagataaa aacgataaat
    34621 ttctctttta attaagagaa attttttgac tttcaaggat attaatataa agttaagata
    34681 taaacataaa gcaaataaat aaaaaagagg tggcaatatg atttatacag tgacattaaa
    34741 tccatcaata gattatatag ttaagctaga tgagctaaag acaggaagta caaatcgtgt
    34801 aaaagaagaa tacgtttatc caggaggaaa aggtataaat gtatctcgca tacttaagga
    34861 acttggaaat gataatacat ctttaggttt tataagtggt tttactggag aatatataat
    34921 aagaacattg gaagaaaaag aattgaagac agattttata aagataaaaa gtggtttttc
    34981 aagaataaat gtaaaaataa aggcgttaaa ggaaactgag attaatggac aaggtccaaa
    35041 tatagatgat gaagacattg atactttata taaaaaactt gataagctaa atcaagatga
    35101 tattttaata ttggcaggaa gtataccatc tactttagat gaaaaactat acgaaaatat
    35161 aatggctaga ttagaaaaaa agaatataaa agttgtagta gatgcaacta aaaatcttct
    35221 tcttaatgtt ttaaaataca aaccttttct aattaagcct aataacgacg aattagaaga
    35281 actatttggt gttaaattaa attcaattga agacatggta aaatatgcta gaaggttaaa
    35341 ggaaatggga gctataaatg tcttggtatc aatgggaaaa gatggagcat tgttgattac
    35401 agaggaagat gaagtgctta taagtgatgt tccaaaagga aaagtaaaaa attctgtagg
    35461 agcaggtgat tcaatggtag caggatttat atctgggtat ttgaatactg ggaaatatga
    35521 ttatgctctt aaattgggag cagcttcagg tagtgcaact gctttttcat atgacttagc
    35581 caaaagagaa tacattgata aattggtaaa tgaaatatct gtaaagaagt tttaaaatat
    35641 taaaaaatat atagaaaggt aattaatatt tatgaaaatt atagatttat tagatgaaaa
    35701 gtcaattaag ttaaatttaa attcaaaaac caaatcagaa gcaatagaag aattagtaga
    35761 tttggtcgca aatagtggaa atttaaatga taaagaaaac tacaaaaaag caattttggc
    35821 tcgtgaagaa atgagtacaa caggaattgg agaaggtgta gctatacctc atgctaaaaa
    35881 ttcttcagtt actaaagctt gtatagcagc agcagtgtca aaagaaggta tagattatga
    35941 atcgtttgac ggaagtttat caaatctatt ttttatgata gctgcaccag acggagctaa
    36001 caatactcat cttgaagtat tatcaagatt atctacaata cttatggatg aagactttag
    36061 aaataaactt ataaattcaa gctctgaaaa agaatttaag gaaataatag ataaaaagga
    36121 aagagaaaaa ttcagtgaag aatatcaaga tgagaaagtg gctgaaaaaa atataataga
    36181 tactaaagag aatgatgcta ataagtatcc taaggtttta gcagtaacag catgtccaac
    36241 tggaatagca catacattta tggcagctga aagtttaaat aaaatggcag aaaacaaagg
    36301 tgtatcaata aaagtagaaa ctaatggttc agcaggtgtt aaaaataagc ttacaaaaga
    36361 tgaaatagaa aatgcaacat gtataatagt ggcagcagat aaaaatgttg agatggctag
    36421 atttaatggc aaaaaagtca taaaaactaa ggtggctgat ggtatacaca aagcggaaga
    36481 acttatagac aaggctgtaa atggagatgc tccaatttac catggaggag atggaagtca
    36541 caacgaatca aatgaagaaa gtgaaagtgg atttagaaaa gtatacaaac acttaatgaa
    36601 tggtgtatct aatatgttac catttgttat tggtggaggg atacttatag cgatagcctt
    36661 tttacttgat gattatacta ttaacccttc aaactttggt tcaaatacac ctatagcagc
    36721 attttttaaa ggaataggag ataaagcttt tggatttatg ttacctgtac ttgctggata
    36781 tatagcatac tctatatctg atagaccagc gtttgttgta ggatttgtag gaggtgctct
    36841 agcaggtgat ggtggttcag gctttttagg tgcactactt gcaggtttta tagcaggtta
    36901 tttagtagaa gggcttaaga aaatatttag tgtactacca gcttcattgg aaggaataaa
    36961 accagtattg ttatatcctt tacttggaac acttttgatg ggaattataa tgacattttt
    37021 agtaatacct ccagttacag caattaataa tgcaatggtt ggatttttaa atggtttagg
    37081 tggaacttca aaaatagttt taggattagt attaggtgga atgatggctg tggatatggg
    37141 tggacctgta aataaagcag catatgtatt tggagttgca tcacttgaat cagggcagtt
    37201 tgaaataatg gcagcagtaa tggctggtgg tatggttccg ccacttgcta tagctcttgc
    37261 aactacattt tttaagaata gatttactaa agaagaaaga gattctggta aagttaatta
    37321 tgtaatggga ttatcatttg ttactgaagg agctatacct tttgcagctg gagacccact
    37381 tcatgttata ccagcttgtg ttggaggttc agcagtggca ggagcactta gtatgttgtt
    37441 caacgcagct cttcgtgcac ctcatggagg agtatttgtt ataccagtgg ttacccatcc
    37501 atttgcatat atattagcta tagctgtagg tgctttggtt ggaatgatgc ttttagcatt
    37561 acttaaaaaa ccattaaatc aagaagtata gcaattaaag gatattcaag aacttaaaat
    37621 atgatgtttt ataaaaatta atatattgct gaattatttt aaaataagga ttttatgagg
    37681 ctgttatata acgatttgta attgttatct tgacagtctt ttatttttat ataagtaaaa
    37741 taagtgtgtt ttgctttatt tgta
//
LOCUS       DBGJFU010000024        37227 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.024, whole genome shotgun sequence.
ACCESSION   DBGJFU010000024 DBGJFU010000000
VERSION     DBGJFU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.024"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            104..1732
                     /locus_tag="ACOIAS_001189"
     CDS             104..1732
                     /locus_tag="ACOIAS_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGZ2700906.1"
                     /translation="MILNYKYNKYIDEYFSLADKGEIVLCKEMKLAVKKIKKDLKKPN
                     IYIDHEKVEKAIEYMEKYFYKMYAWEKFVVALVVGCFEGDELLYDEIFIMMGRGNGKN
                     GFISQLANYLQTHLHGVKNYGIDIVAMSEEQAKTSFDDVYNMLSEHPAICKKFFNRTK
                     EVITFKKTNSYLKFRTNNAKSKDGLRPGCIIFDEVHAYENYDNIKVFTSALGKVKNPR
                     IFYITTDGNVRGGVLDDFKEEAKQILNEEIKNSKMLPLIFKLDSIEEMEDVNLLEKAN
                     PSIKYNSDLRTQILKDIDKAKNRPQMMIELLTKRFNLPAEDLATVVAEWDDIVATNEE
                     LPDLKGFSCIGGLDFSSIRDFTSVVLLFKKDKKRYIMHHTFICYKSLEITNFKFDIEL
                     AKQKGLCTIIYDETIKAEYIADWFIEKAEDYNILKICCDSYRAAHVKEEFTNKGLPIE
                     EVRNGSITHAKIAPLVEQIFAERTIKWGDDMMMRWYTNNVYVDTDKKGNKTFLKIEPI
                     KRKTDGFFAFLHALSKDDELMQESTIKFYDCYTY"
     gene            1748..2935
                     /locus_tag="ACOIAS_001190"
     CDS             1748..2935
                     /locus_tag="ACOIAS_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGZ2700907.1"
                     /translation="MNFKNFISNFLFKDKMSGADGEYFKKLEATIFYKEFALKSCISI
                     IANALVLSEFQTFKNGKSIKEKNYYLFNIEPNQNQNATEFWQEVITRLVYDNECLVIQ
                     ENEQLFVAESFSVDKYVFYEDIYKDVTIRNYKLDRSFRESDVLYFKLNVESIKTVIDY
                     LYNDYKDLLSSAMKGYKKNNSEKGVLEIDTNYPQTKEAQERLNDLMSNKFKRYFESDN
                     AVLPLSNGLKYTENTKASSIKDSRDIRAIIDDVMDFVCAAFHVPAGLVKGNVAGVEGI
                     TDNFLNFSVNPVAKLITNELTRKMYGRDNFFEKSYVKVDTQKIANMGLEKISKASDLL
                     FRIGVNSINDNLEMLGREKINESWADEHYVTKNYQSILNPDLKGGANNGNGEKNGVAG
                     NKG"
     gene            2892..3527
                     /locus_tag="ACOIAS_001191"
     CDS             2892..3527
                     /locus_tag="ACOIAS_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGZ2700908.1"
                     /translation="MEMEKRTVSLEIRDSNLESRKISGYAAVFSDDYTKLQDRWGDTF
                     YEKISRGAFLKTLADNTRDKFMLINHDWNKVVGRTNSNLILEEDEHGLRFEVDIPATT
                     DGNDLLENVRLGLIKGCSFGFNIVNSKTRWDDDWTFYRDITEVELFEITATPIPAYND
                     TEINCRSDISIKELRESQKEETKQNDNKDNIEKRNVDLVSAFFNAFNRGGK"
     gene            3530..4696
                     /locus_tag="ACOIAS_001192"
     CDS             3530..4696
                     /locus_tag="ACOIAS_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGZ2700909.1"
                     /translation="MAIKNLDEATNVELRADLMEAIKGNDEEKITTAFLRMAEDIQEN
                     LLKEARSIANIESADRAILDKRGMPQLTSEERAYYSKVIEKRGFTNLEITMPRTVFDR
                     VFDDLEQNHPLLSAITFENTTGVTEWIIRKTNAEAAWWGRLDDTIKKELEHGFDKINT
                     EAYKLSAYLPVAKAMLDLGAEWLDRYVRVVLTESISLGLEMGIVAGTGKDQPKGMIKD
                     LKGAVVEGVFPDKAAKPLADFKPITLGKEIMAPLTNNGKRAVPSVIMLVNPVDYWAKI
                     FAATTMLTSNGTYVYGVLPIPGNIIQSVAVPKGKLIAGMAKDYFMGVASSGKIEYSDE
                     YKFLEDERTYLVKQYATGAPKDNDSFLVFDIENLDTTLATEINSNTKTSAARAK"
     gene            4706..4993
                     /locus_tag="ACOIAS_001193"
     CDS             4706..4993
                     /locus_tag="ACOIAS_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700910.1"
                     /translation="MENENIKSVVEEVKEYLNITWIEEDAKIEKMVVRGIDYFENEIA
                     GIALDFKDNFNRELLFNYCRYVRNNAIEYFEENFSKELLRLQIKSAANEVI"
     gene            4996..5646
                     /locus_tag="ACOIAS_001194"
     CDS             4996..5646
                     /locus_tag="ACOIAS_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HGZ2700911.1"
                     /translation="MKKIKFNNFNDGIVEFGEYKESYDIEGNALDEKEFIVSGKLFYS
                     NEYIREQDKLKFDDTGLKVSIKLKVPYMRLIKTSDVIKLNNEFYSIVKLDISNDKTSV
                     YLYLTELVDVLDKHISIFIKERKNVLEDVTIKFYKKIWACVEDLNSKELIENNSIKTS
                     TNKKFKIRYIEELDLSIKQNAITNFVIKYKDKFYNITQILNLEEENKILEITAESK"
     gene            5666..5992
                     /locus_tag="ACOIAS_001195"
     CDS             5666..5992
                     /locus_tag="ACOIAS_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700912.1"
                     /translation="MHSKIVTILKKLNLGIGFQEYDNFSNDEEYIIFFIENEYDANFS
                     DNENKSIVYSIKIEYWYKSLKNINKYISIKDIFKKENFIFLNSSDFIENEYYCKELKF
                     KYEEVV"
     gene            5993..6436
                     /locus_tag="ACOIAS_001196"
     CDS             5993..6436
                     /locus_tag="ACOIAS_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGZ2700913.1"
                     /translation="MSVEITTEGFDAILSKIESMGKTGDKLLNEAVKAGGNVILQDAL
                     PRVSKRSGKLKDGLKVSGVKKKGGTKYVLVGITKEDNSEIFYGKFLEFGASAHKIPIK
                     KGKKKGRVVNHPGTSSKPFLAPAYESKKDEAKNVMKEILKRGLGL"
     gene            6436..6786
                     /locus_tag="ACOIAS_001197"
     CDS             6436..6786
                     /locus_tag="ACOIAS_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700914.1"
                     /translation="MININDLVIETLKPLKIPVSFQRYNGEEKTYITFFSYLEQNFYA
                     NDEVIGTEHYIQIDLFSKNKMSYINKEIERLLKKNNFIKRSIHEIKESDYSYHAVFRF
                     LFLQKKRRIKKWLL"
     gene            6774..7370
                     /locus_tag="ACOIAS_001198"
     CDS             6774..7370
                     /locus_tag="ACOIAS_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HGZ2700915.1"
                     /translation="MAIIGLRNYRIVKLLTDTEETLEYDTKITRLTGAKSVKISPKVD
                     SAENYGDDQLLETASAMGAIEVEIEVADLTLEERALILGYQYKDGVLIEDKNFNPPNL
                     AFGFESPKSQNGSRMVWLTKGVCEPFEEEAKTKEDKIEFQSQKIKLKFMPRIHDGRHK
                     ITADTDVENAPTDAQFFTTDFLKTGVKPTTPAKANLNK"
     gene            7385..7672
                     /locus_tag="ACOIAS_001199"
     CDS             7385..7672
                     /locus_tag="ACOIAS_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700916.1"
                     /translation="MEIKLTINEEEKIFKAPPIPLRKLDKVFTLTDKIEDGLNSTELF
                     RELLDFTVDIYGEQFSKDELLDGFYPAGDFINKALEDLSKVSGGFEEKVKN"
     gene            7924..8139
                     /locus_tag="ACOIAS_001200"
     CDS             7924..8139
                     /locus_tag="ACOIAS_001200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700917.1"
                     /translation="MCKYCEVEESEAAFKRGKTNRGELITCDCDIEMWIEKESNEYFL
                     NSECWCGYYSESFEPINFCPFCGRTLI"
     gene            8202..13403
                     /locus_tag="ACOIAS_001201"
     CDS             8202..13403
                     /locus_tag="ACOIAS_001201"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGZ2700918.1"
                     /translation="MAEDVGELVVRVAMENSSFQQGINNLNRQMKLIRSEFKNSGAGV
                     KNFGTSLDGLKSKQQMLSSTIQQQTKVVEAYKNKLNESKTTLSNTAQKQVELKEKVNQ
                     AKSAYEQSKATLGENAEETKRLKSELDSLEQEYAKNEEKIRSNSAAVDNWSIRVNNAE
                     SRLSEMRSELDDTTERINQQENKWNQLATKMTEIGNKFSSVGKQMQDVGKKMAMSITA
                     PVLAVGAAASKLGMDFEASMSNVQGLSGATADEMVQLEKAAREAGASTSKTAKDAADA
                     LGYMALAGWDAKTSMEALMPVLRLSEAGNLDLARTSDLVTDSMSSLGLSVKELPTYLD
                     QVAKTAASSNTNIDALMEAMIVSGGTFKNLNVPLSEANALLGILANRGLKGSEAGNSL
                     NSIMINLTSGAGQAGVAMKELGLSAFDSNGKFKGMANVLMELKEKTKDMTEEQRTMYL
                     SMIGGKTQISTLQALLSGVGEEYSELEKKVGNSTGALDKMAKTMQNNNKGSITALISA
                     VQELGLKIYDVLKPSIAQVVSWFQNLTNKLNEMKPATVETIVKIAGLAAALGPVLLIG
                     GKIVSMIGGVIGAFGTFSAAMAVATTGAAAATPAVGALAAILGALSSPAAIAVAGITA
                     LGISVATVAKDMSKDAIEPISRFGKSVSESTKEAVGAFMDLEEKTTISLNQLMWSGET
                     VSEKMKQSIVSNFTEMSNQIIAKLQESKEQSIQSLQEMFATSKNLSDKEKEELIKNTE
                     EAYSSKEEKIKKSNEKVNQIMTKASQEHRALTKEESAEINQIKTDMLNTAVDTMSKSE
                     AEQAAIMERMKANHVDLSAKEAAEVVKNSIKKKDESIKAANEEYNERLKIAAQLRAEG
                     GAENEKLADKVVEEATRQKDESINKAKEMHQGVVAEAQKQAENQASKVDWTTGEIKSK
                     WQVLNDTVKGKMQEWDDMAGNWVEGLKEKISTGWANLKENTAILWDNIKLTVTTKWTE
                     IKESIGLKIEEIKQNISEKWESIKLFFTTTWENIKMIFGNAWDAIKQQVEEKFAPFVE
                     RLKIIFDGIKEFFRSTWEVIKNIVLGAVLLILDLVTGNFDKLKTDAIQIWENIKQSLS
                     DAWESIKEVFNNALTAISDLLSNMWEGMKSTASSVWESITQTLSSIWENIKSTCSNAW
                     EGLKTICSNTWTNIKTTLVTSWENMKTAVSTKLTEIKNNIVTVWNSVITFFKTLPAKL
                     KQAAIDMFTRLREGIDSKKQDAVNASQKVGTDVVNKIKEFPSKFLQVGKDFVSGLANG
                     ISGFAYKVVDEAKKLGQKAADAVRKVLGIHSPSRVMAEIGKFVDEGLAQGIANNSDSV
                     KKATEKITKIIEEETKKATKACEEDIKLFNKQIDELSKQEKAALKKVKGDAKDALKEE
                     YAAKKEKIKEAIALRKEQKEKEVEQLKEIANSAKEQLNKELQDRQEFVKKVDDLTKAI
                     VDALKEKYKQEYEAQQASIKKELDALDKWKEESIDRINSVYDAKIKAIDNELEAFEKA
                     EKEKDRLEQDKEELNKIEEIKTAIKFEHDDTNKEQLQKELEKVLAERKKRIEQQEIED
                     KKEALKKRKEELEEKKKDEIENINQIYESEKEYYNKRLEDAKKFYDERIKESKLQAEA
                     EKLIMDKNQKEIVALLNSYSDAYKNAGQTLGEKLLEGFKPAINEIKNLIDSITREINN
                     ARNEALSLNSDIRKGNSAANSNSGRREATYNNNSRTANYNVNVNSTDSNTAREIERTI
                     RSLAFSL"
     gene            13435..14301
                     /locus_tag="ACOIAS_001202"
     CDS             13435..14301
                     /locus_tag="ACOIAS_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HGZ2700919.1"
                     /translation="MQKLICRNSKGQEVKLGNSRPFLLEKIENGANTSANISVSNNLG
                     DGVSIDDISIKERLLPISAAIYAKNKEDLELKRAYLTSLFNPKLGEMEFIYTNNALTK
                     RVKGVVQDITFQTPVGLMQKFLIQLLTPSPFWMDEFVLKEEVALWVGDFEFTLEISNE
                     GIEMGHRVSNLICNVVNNGDVECGMKIQFKALATVDNPSLFNVNTREYIKINRTLEAG
                     DLLEITTEFANKRIELVKSNYERINVFNWIDLDSEFLQLEVGDNLFRYDADVGIDNLE
                     MSIYYNPLYLGV"
     gene            14303..15382
                     /locus_tag="ACOIAS_001203"
     CDS             14303..15382
                     /locus_tag="ACOIAS_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus ReqiPepy6 Gp37-like family protein"
                     /protein_id="HGZ2700920.1"
                     /translation="MQQSIRILDKNINLIAEIDNYEELQIIKKFHKVGEFSLKINANK
                     NHVDKLVKNNIILLGKNYNKVCLILHREFMYTETGEKSDILSVKGVDLKGLLNRRLII
                     PDTGEAFESHEGTQEEIIKAFVNNNCVDPSNSKRVIDNLIISENKNLGSADMWRSSYE
                     NLSDKIQEISEFCGLGWEVVLDANKKKFIFDVITGKDLTINQTENPPIIFRSDFNNIR
                     ARHFTESIINSKNVVYAGAREDATKLVISTGEVEGFERNEVFADVSENTISILQKEGE
                     IKLKEYEELKSFELEIDPKNTFMYEKDYKLGDIVTVQDRKLKVTMDTRIVEVQESYSK
                     NGIKLKITFGSSIPTLFSKIKRMVK"
     gene            15382..16263
                     /locus_tag="ACOIAS_001204"
     CDS             15382..16263
                     /locus_tag="ACOIAS_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700921.1"
                     /translation="MEKSFVFNSVNGDRRYKAEDFREYFASFIGNGVFPNPSSNLQVV
                     DNNNMTITVKKGKGWINGAIYINTDDLVINIDPADGILNRIDRVVLRFDTLNRNIKLA
                     VKKSAFNSSPAATELQRDADAYELGLADIYIRAGAISITQSSITDLRLDKNLCGIVKG
                     TIEEIDTTTLLAQLSTWKDEEISNFNDWRENQENKQEDWYNTTTTNFVSEFNIWFESI
                     KDILDEDAAGNLLNEINKVKEELAKIETTAEKTSYNNTTSNLTATNVQGAIDEVVRKI
                     EKFNEVNISIQNDMLPI"
     gene            16280..17971
                     /locus_tag="ACOIAS_001205"
     CDS             16280..17971
                     /locus_tag="ACOIAS_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine rich domain-containing protein"
                     /protein_id="HGZ2700922.1"
                     /translation="MAIVYEFNYTGAEQSVVLPPGKYKFECFGACGGNYYDFVQCARG
                     GYTAGSLILKENTTLHVYVGQSGYCKGVNGIETCRSGFNGAGGITTYKSTSDGYYSLA
                     GGGATDIRLIGGNWDNLQSLLSRIIVAGGGGGGSGNSHDSIGHGGGTKGKDGISIANK
                     YFAGGGSQFQGGLTFNSLYNGSFGVSGAGDGISGVGGGGGWYCGAGSFYAEFGGGGSG
                     YILTKDSYKPANYSPSSKYYFSDINSVVGGNTTKQDGYAKITLLQALPFLTISSYNST
                     TATFKADHTDTTLLTKIEYFIDDVLKETITTDLTAEKTINYTVEDNALHALKIVVTDS
                     ANATAEKVVSISKGIAPLPAGSTTDEVTSKWMEIKDTFKSGKTSIINTLALKNIEASL
                     NNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTISQLNSKYKVASGNSVVNYCTNT
                     KGFYFNSDYLFLFPGAIQINGLNFVPNIFFTTFELIDDGYFHKYFVFACRGIFTQDFV
                     ITAHYYRLKSYLQDFKVGGEVLKLNERDVYMDNRGIQLPCSRQGSSFKWQAIKFI"
     gene            17988..18281
                     /locus_tag="ACOIAS_001206"
     CDS             17988..18281
                     /locus_tag="ACOIAS_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700923.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILEHDTITELHYIDVEYGS
                     IDYTRNRIIGINIETKEPILEEIPVFISEEEKRIQELENQILLNENEKVGGIL"
     gene            18281..18463
                     /locus_tag="ACOIAS_001207"
     CDS             18281..18463
                     /locus_tag="ACOIAS_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700924.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIEHSGVVEGTQPTK"
     gene            18525..18956
                     /locus_tag="ACOIAS_001208"
     CDS             18525..18956
                     /locus_tag="ACOIAS_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGZ2700925.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGSK"
     gene            18956..19795
                     /locus_tag="ACOIAS_001209"
     CDS             18956..19795
                     /locus_tag="ACOIAS_001209"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2700926.1"
                     /translation="MKICITVGHSILKSGACTSANGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKIYKIPRVNSGGYDLLIELHLNSSGVGAFGTEVFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGVKLDKSLYILNSSKPTAILIESFFCDNK
                     EDYEKAKKLGHEGIAKLIVEGVLNKNINNEGVKQMYKHTIIYDGEVDKIPATVVGWGY
                     NDGKILICDIKDYVPGQTQNLYVVGGGACEKISSITKEHYTMIKGNDRFDTLHKVLDF
                     IKK"
     gene            complement(19822..20031)
                     /locus_tag="ACOIAS_001210"
     CDS             complement(19822..20031)
                     /locus_tag="ACOIAS_001210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700927.1"
                     /translation="MKDIFEELISIKDACELYKKGESTLRLNIRNKKFVEGIDCKKFG
                     TTWVFKKTALEREYNKKDKKRESGN"
     gene            20184..20633
                     /locus_tag="ACOIAS_001211"
     CDS             20184..20633
                     /locus_tag="ACOIAS_001211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700928.1"
                     /translation="MEKFIRLDFDKGFRGKEHRSSATGDGEHFEAGISCYKINKEKCV
                     DAIINLCEYWFEFAGECQFKDFDINIFEGYYVGEGASYEDLATCENHLHCVDGSLFNE
                     VYDLYYMHETYLEENRDIEELEENYKDEYITTEEFETKIKEMFIKYL"
     gene            20645..21286
                     /locus_tag="ACOIAS_001212"
     CDS             20645..21286
                     /locus_tag="ACOIAS_001212"
                     /inference="COORDINATES: protein motif:HMM:NF013043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HGZ2700929.1"
                     /translation="MKIGDKFENLTILDIEQRNSRKYCLCKCGNCGNEKWIRADSLKR
                     IKACGCMKSNTQFKQNDLTGKKFGRLTAIKNTNKKAKSGHYIWICKCACGNEIKTTEN
                     NLTTGRTKSCGCLKKESNIKNAKIALKVHKEKNIIDDTNLSIIKKTEAYPNSKTKIRG
                     VYYNKEKRKYCAQIEFKKTHYNLGYYDNIEDAEKAYIEAKDNLHKKFLEKLHE"
     gene            21326..21802
                     /locus_tag="ACOIAS_001213"
     CDS             21326..21802
                     /locus_tag="ACOIAS_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700930.1"
                     /translation="MNKQKAKRFLRAIDMNIDKIEEEATKIYKERYLIETTDAIRISI
                     NLEGVVKSTISSWQGYSDDIFNMREVIVYEFSQEKVQIDDFLGELCFLNDYEEFATWC
                     DTESETLNWNSYEKFNKENFDELVERNIEDGLPIFLKELSESIENCKQDLKLMIEI"
     gene            complement(22047..22328)
                     /locus_tag="ACOIAS_001214"
     CDS             complement(22047..22328)
                     /locus_tag="ACOIAS_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700931.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVTNYLRFAALDNDMKIKL
                     FQTIIEAVARKKVSLPNDSEFTEGILQSSELEMTTNVGFIIEGLSYHNN"
     gene            complement(22549..22908)
                     /locus_tag="ACOIAS_001215"
     CDS             complement(22549..22908)
                     /locus_tag="ACOIAS_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700932.1"
                     /translation="MKFDSEKNENKVDYRDITEKRIQDTFKKNSNSIVTSLLEVKENE
                     EVKKESISIYFESDDINVLKAVAQIKKTTINKLVLDVLKPVIDTTRIDLEDSEDIEKL
                     AKSYDKRKKGRGRKIVK"
     gene            complement(22910..23728)
                     /locus_tag="ACOIAS_001216"
     CDS             complement(22910..23728)
                     /locus_tag="ACOIAS_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HGZ2700933.1"
                     /translation="MSTSLNTISIFNVKGGVGKTTLTILTAMYLSKLNKKVLIVDGDF
                     QANTTQFIHNTLFEGNTMFEALAYKTKAEDIIIKSPLKDFPSIDLIGSKLECCVLGEL
                     LVTETNREKAVYRWFAKNIGTLGKYDYILIDLSPSYDVVTRNFLLISDSIVTPLEFQD
                     IASIRGCNLFYTKFKEDLEKLEIDNNVKKAIVVNRYTSRKLSTGDEFNNQLEKYDNIK
                     KVLLNTRISEGTIVKNAILNKTDIEEYCKKIKKAHKVREEFKNYIDELFERGIL"
     gene            complement(23967..24149)
                     /locus_tag="ACOIAS_001217"
     CDS             complement(23967..24149)
                     /locus_tag="ACOIAS_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HGZ2700934.1"
                     /translation="MKKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNE"
     gene            complement(24388..24516)
                     /locus_tag="ACOIAS_001218"
     CDS             complement(24388..24516)
                     /locus_tag="ACOIAS_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700935.1"
                     /translation="MGRLERSKKKRENKFNIVKKVFSFILLLLNIILAVLRILKEL"
     gene            25529..26221
                     /locus_tag="ACOIAS_001219"
     CDS             25529..26221
                     /locus_tag="ACOIAS_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700936.1"
                     /translation="MSLEEAKELEKNYKLPMPTYCLNEKNYCAKEFGALMLISKGSMR
                     IMHIEKNRYLYQNKFKMEELAKQIGVARTTLERNIKKLNSLDCKVLEIENSRNGIIYR
                     LNYGTSTGYNDNVHKFVTIHHDMLQELISAFNTNAIKVYCLLCYMTTENSFKCMTEKF
                     ICEKIGLCGDSKNNRSKIRKIITVFEVSKYIEVKKENKFEWDEEKNKKVPRIQKLYRL
                     CSFSEWKNARKK"
     gene            26885..27511
                     /locus_tag="ACOIAS_001220"
     CDS             26885..27511
                     /locus_tag="ACOIAS_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700937.1"
                     /translation="MKKVLLLITVCLLSVGLVACSSTNNKDTGNTTKKEKKANENEVD
                     GIKITVKDVTKEDIKGDINEDGSFNENGEYFSDGIEKKKAADYVYEVVNVEVENKTDK
                     AVKLFQTGWNAVGTDGYEFKDIKVTDKLDNQQVPANYKFDAQVKILVEKNMNVKEIVL
                     KYNLKDYSRLFEAMEYANQGASKSDVEKKFPELYKDNWIELGEIKVEQ"
     gene            complement(27538..27837)
                     /locus_tag="ACOIAS_001221"
     CDS             complement(27538..27837)
                     /locus_tag="ACOIAS_001221"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700938.1"
                     /translation="MESLLKYHRKKKKLTQRRLSELTGISQSYISRMENKKFKHSPTL
                     TQIILLADILEIDQLILSEFFLEKEREHLKNTKKYNKKAVKTLSRDFLKEYKNKD"
     gene            complement(28209..28577)
                     /locus_tag="ACOIAS_001222"
     CDS             complement(28209..28577)
                     /locus_tag="ACOIAS_001222"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700939.1"
                     /translation="MIDKRIRELRLKLNLSQDEFANELTLKRNTISLIENGKRNTSER
                     TLNDICKKWNVSEEWLKNGTGEMFTIKDLDEQYASLVGELSKKENEVEREIILKMFKL
                     NKKYLTVFDKMIDSLLEIEE"
     gene            28723..28863
                     /locus_tag="ACOIAS_001223"
     CDS             28723..28863
                     /locus_tag="ACOIAS_001223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700940.1"
                     /translation="MVNINEMREEIYRKIELLDPTKLKLALNRVNILKEIQDIEEENI
                     QN"
     gene            28876..29091
                     /locus_tag="ACOIAS_001224"
     CDS             28876..29091
                     /locus_tag="ACOIAS_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700941.1"
                     /translation="MNISNFNKYQKGEIVQLYETKFSCNRNCIVTYADEKELVLMYYD
                     KEIEELIYKTLTKEDLIFNDYELKLLS"
     gene            29121..30284
                     /locus_tag="ACOIAS_001225"
     CDS             29121..30284
                     /locus_tag="ACOIAS_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HGZ2700942.1"
                     /translation="MRFSIHGFDQKTAVEFGLTNDELLILRHFEDFVHSGKMDSFYEE
                     GYMYYWVNYNKFLEDLPILKISKDRLGDIMLHVLGEKPIELEEKMKCYSEKMLKKVKN
                     RKYIGLLKNKTIRNPVAGVRSYFAFTLKFYKLKQKITNNDDSINTDSVSIPERFGINT
                     DIDSVSIPEPNRYQYRNKDYSINDYSINDIDRYSPLNKQEVQHTMPNVRNEVLNPTAC
                     GAVHVRNEVPSNKTNINKTNLTNNIYSSVEQKEEKTKDKEIYKRIVDYLNKTADKSFK
                     STTKKTISLIDARLKEGFTEEEFYKVIDNKVLNWLDDDKMNAYLRPETLFGNKFESYL
                     NETPKKSLKGNESVKGKTKTNNNKFANFNQTFDKYSEDELDAIIKKSQSAKFK"
     gene            30309..30911
                     /locus_tag="ACOIAS_001226"
     CDS             30309..30911
                     /locus_tag="ACOIAS_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700943.1"
                     /translation="MEINSVRELRELFELFTVIKAEGNNVVSCNETVNELSNKELIKA
                     NENKFKKPWNDALLYEINALDIIKKHKHSDRKEKAYKTYLGLENADIEEIYKIHYGDK
                     ENSETVASCEEISSAKTDTISCNHDSVSKGKDTSDSECTCFICSKKIENQEDVFKIPF
                     LTRDYLSSAKEIKNIEICEKCLDGNSWSIIRRKQFKKMKK"
     gene            30957..31238
                     /locus_tag="ACOIAS_001227"
     CDS             30957..31238
                     /locus_tag="ACOIAS_001227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700944.1"
                     /translation="MIKNNVLSYEEKIKVLKKHGYSDFTKEYVLKEFDKEHAFYKSFL
                     RAIEKEDDGKGVLIKKIGKGQHQFSKGFIKTHTISELLEEAKRIRKILE"
     gene            complement(31277..31621)
                     /locus_tag="ACOIAS_001228"
     CDS             complement(31277..31621)
                     /locus_tag="ACOIAS_001228"
                     /inference="COORDINATES: protein motif:HMM:NF022172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HGZ2700945.1"
                     /translation="MVTNQDCIRDILLLIESNTTPISPVLQFDTILDNLTYDKSTIVH
                     HINLIYQNNLVDDVNYGDDTVLEVMQLSLKGYQYLDKIRDNTKWNKIKSSTSSFLNMS
                     LPIAIEYILGKF"
     gene            31710..31886
                     /locus_tag="ACOIAS_001229"
     CDS             31710..31886
                     /locus_tag="ACOIAS_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700946.1"
                     /translation="MQKRITEMSILEKVDLIGQKQKKLQEKYKNLSKGELFSIACNEV
                     KKIEEQEGKYFEYR"
     gene            31873..32238
                     /locus_tag="ACOIAS_001230"
     CDS             31873..32238
                     /locus_tag="ACOIAS_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700947.1"
                     /translation="MNIGSYTLVKKQLVKELEEENKQKEECLKEREKHIKELEESLEY
                     FARDALESEECIIDIENILIEQAVELEAYNLFIDCILNKDIRKAIAIYNRTKSIRIKH
                     KNINSISKKIEKIKQIISL"
     gene            32258..32434
                     /locus_tag="ACOIAS_001231"
     CDS             32258..32434
                     /locus_tag="ACOIAS_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700948.1"
                     /translation="MDKFVTCARCERTINVEENNYVKYEEETLNLSFTLYFCLGCVDK
                     LIEEQLKSMEGENE"
     gene            32427..32642
                     /locus_tag="ACOIAS_001232"
     CDS             32427..32642
                     /locus_tag="ACOIAS_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700949.1"
                     /translation="MNKISVTQALEKFDSLLDNWNDLPNHVYKKEYRGKFYDWIKSLE
                     RKDSLQNYKIVEVLNNERNGEEAPFWN"
     gene            32701..32874
                     /locus_tag="ACOIAS_001233"
     CDS             32701..32874
                     /locus_tag="ACOIAS_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HGZ2700950.1"
                     /translation="MKMINVFKLSSMYCICPKCGSDKLGKNEGKLIVDEHTYYRECKC
                     GFNVLVDEREDEI"
     gene            32889..33296
                     /locus_tag="ACOIAS_001234"
     CDS             32889..33296
                     /locus_tag="ACOIAS_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGZ2700951.1"
                     /translation="MNSVILIGRLTKDPELKYIPGSGTAVSTFTIAIDRDYIKKDGTK
                     ETDFIPIEVMGKLAEVCANNLGKGRLVAVEGSIRVNSYEKDGEKRTYTKVHANKIKFL
                     DYKKDDTEKEYMFEPKGLEQQGFQAIDDPDIPF"
     gene            33313..33462
                     /locus_tag="ACOIAS_001235"
     CDS             33313..33462
                     /locus_tag="ACOIAS_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700952.1"
                     /translation="MSNTNKKTAKICGWCGTTIYSYMDGKIAYCSKKCKKKREKNLET
                     EGAKK"
     gene            33459..33719
                     /locus_tag="ACOIAS_001236"
     CDS             33459..33719
                     /locus_tag="ACOIAS_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700953.1"
                     /translation="MKEYRIEIGEMIFLKHKVIVEVPEQICAFDIYKYIENECQNKSE
                     ISEYIEKIGGKQLNFIEGDSGDSDLTVESCKRIKKGGKKNGK"
     gene            33999..34259
                     /locus_tag="ACOIAS_001237"
     CDS             33999..34259
                     /locus_tag="ACOIAS_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700954.1"
                     /translation="MNFKMIANIPNWTAKEQFEKVLEEVLELKEAVHLKDNKKIMEEG
                     LDVIQAIFTLFKVLKIDKELSEGLKLHNKKLRKRKWKLEKLK"
     gene            34261..34431
                     /locus_tag="ACOIAS_001238"
     CDS             34261..34431
                     /locus_tag="ACOIAS_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700955.1"
                     /translation="MGKVEYIVFYYNCETFEVCKKSFSALTDAKVFKNEIIEEYESVD
                     IIKRTVFEELIL"
     gene            34712..35173
                     /locus_tag="ACOIAS_001239"
     CDS             34712..35173
                     /locus_tag="ACOIAS_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700956.1"
                     /translation="MKIKKEYFDKVENALKMLGDKYRKLRVLKKEIAILKHKENYREV
                     NYEELGFKVQKSVKGIDDMVVTIEDAIYNKETEIEIIERKLEFYNIYLKELSEIEQKI
                     IELVYFYSWDGKMPITKMARELNYDRTSLYNKKAIAINKIALMIYGDEALE"
     gene            35354..35902
                     /locus_tag="ACOIAS_001240"
     CDS             35354..35902
                     /locus_tag="ACOIAS_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGZ2700957.1"
                     /translation="MNFVEPIRDLEKLESICAYLEETNMRNNVLFSMGIYTGLRVGDI
                     LKLRIKDVHDKRFITLKESKTGKTKPIEVNPILRRILKEYTKDKNQNEFLIKSREQTN
                     KAISRVMAYKIIREVGEMFGIDNLGTHTMRKTFGYHYYKRTHDVVTLQKLFNHSSATI
                     TLRYIGIEQDDLNKAYRNIKYF"
     gene            36075..36347
                     /locus_tag="ACOIAS_001241"
     CDS             36075..36347
                     /locus_tag="ACOIAS_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700958.1"
                     /translation="MNKYGKFKCNNCGKKVNVNNRDLKTKVIDNTTVTYFMCRRCFEK
                     FVVSYEDDYIKHKREEYKKLKDDEVKLLQDMKEYSDGLGELYINEL"
     gene            36337..36669
                     /locus_tag="ACOIAS_001242"
     CDS             36337..36669
                     /locus_tag="ACOIAS_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGZ2700959.1"
                     /translation="MSYKHMTLDRLKELILIDRLDIFYRSTDWRKLRKKALERDNNEC
                     QICKEKKKVSKAECVHHIKEIKEYPSEALKLNNLTSLCNLCHNEIHEKILKINQEKKK
                     KFFSEERW"
     gene            36841..>37227
                     /locus_tag="ACOIAS_001243"
     CDS             36841..>37227
                     /locus_tag="ACOIAS_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HGZ2700960.1"
                     /translation="MDDNEELEIRRDVQNDYVLGMTYKEMSAKYNIPENTLKSWRKRG
                     GWTRNCTPKKGCVKNGLQESGNNLKNSIRKDLLRQIKENNMTEEYFKDLVEDYLKMWD
                     IKNELIEDVKTRGVQVKYKNGQNQWGY"
BASE COUNT        15204 a         4144 c         6628 g        11251 t
ORIGIN      
        1 aaaaaaaacg atagtgtatc agagcttatc aagtataacg ctcaaatgtt aagtttactt
       61 gataaactag gtataaaagc gacgccaaaa gttttgggtg atgatgatat tgaattataa
      121 gtacaataaa tacatagatg agtatttcag cttggctgat aaaggcgaaa ttgttctatg
      181 taaagaaatg aaacttgcag ttaagaaaat aaaaaaagat ttaaaaaaac ctaatatcta
      241 tatcgaccat gaaaaagttg aaaaagctat agaatatatg gaaaagtatt tctataaaat
      301 gtatgcttgg gaaaaatttg ttgttgcttt agtggttggc tgctttgaag gcgacgaact
      361 tttatatgat gaaattttta tcatgatggg taggggaaat gggaaaaatg gttttatatc
      421 ccaattagct aattatttac aaacgcactt gcatggtgta aaaaattatg gtattgacat
      481 tgttgcaatg tcagaggaac aagcaaaaac aagttttgat gatgtttata acatgttatc
      541 tgaacatcct gctatatgta aaaagttttt taacagaact aaagaagtaa taacatttaa
      601 aaaaactaac agctatttaa agttccgcac aaataatgct aagtcaaagg atggattaag
      661 acccggctgc attatttttg atgaggttca tgcatacgaa aattatgata atataaaagt
      721 ctttacctct gcgcttggaa aagtaaaaaa tcctagaatt ttttatatta caactgatgg
      781 gaatgtaaga ggtggagtat tagacgattt caaagaagaa gctaaacaaa ttttaaatga
      841 agaaattaaa aactctaaaa tgctgccact tatttttaaa cttgatagta tagaagaaat
      901 ggaagatgtt aatttgctag aaaaggcaaa cccaagtata aagtataatt cagacttaag
      961 gacacaaatt ttaaaagaca ttgacaaagc taaaaataga ccacaaatga tgatagagct
     1021 actaactaaa agatttaatt tgccagctga agatttggca actgttgtcg ctgaatggga
     1081 tgacatagtt gcaacaaacg aagaattacc agacttaaaa ggcttttcgt gcattggagg
     1141 attagatttt tctagtataa gagattttac aagcgtggta ttgttattta aaaaagataa
     1201 aaaacgatat atcatgcatc acacttttat atgttataag tctttagaaa taacaaattt
     1261 taaatttgat attgaattag caaagcaaaa aggtctctgc acaataattt atgatgaaac
     1321 gataaaagcg gaatatattg ctgattggtt cattgaaaaa gcagaagatt ataatatatt
     1381 aaaaatctgt tgtgactctt atagagctgc acatgtaaaa gaagagttta ctaacaaagg
     1441 tttaccgatt gaagaagttc gaaatgggtc gataactcac gcaaaaatag cccctcttgt
     1501 agaacaaatc ttcgcagaga ggacaattaa gtggggcgac gacatgatga tgcgttggta
     1561 tactaataat gtttatgtag atacagacaa aaaaggaaat aaaacttttt taaagataga
     1621 gccaatcaag cggaaaactg atggcttttt tgcgttttta catgctttat caaaagatga
     1681 tgaactgatg caagaatcaa caattaagtt ttatgattgt tatacgtatt aataatgagg
     1741 tggtgaattg aattttaaaa attttataag taatttttta tttaaagata aaatgtcagg
     1801 agcagatgga gaatatttta aaaaactaga agctacgata ttttataaag aatttgcgtt
     1861 aaagtcatgt atttctatta ttgcaaatgc tttagtttta agtgaatttc agacatttaa
     1921 aaatggaaaa tctattaaag aaaaaaatta ttacttgttt aatatagaac ctaaccaaaa
     1981 ccaaaacgca acagaatttt ggcaagaagt aattacaaga cttgtgtatg acaatgaatg
     2041 tctagttata caagaaaacg aacaactttt cgtagctgaa agttttagtg tagataagta
     2101 tgttttttat gaagatatat acaaagatgt tacaattaga aactataaat tagatagaag
     2161 ttttagagag tcagatgttt tatatttcaa attaaatgtt gaaagtataa aaactgtaat
     2221 agattatctt tataatgatt ataaagattt attaagtagt gccatgaagg gttataaaaa
     2281 aaataatagc gaaaagggcg tacttgaaat agacacaaat tacccgcaga caaaagaagc
     2341 gcaggaacga cttaacgatt tgatgagtaa taagtttaaa cgttattttg aaagtgataa
     2401 tgctgtttta ccgctttcaa atggattaaa atatacagaa aatacaaaag cttcaagtat
     2461 aaaagatagc agggatataa gagcgattat tgatgacgtc atggattttg tatgcgctgc
     2521 ttttcatgtt cctgctggac ttgtaaaagg taatgttgct ggagttgaag gtataacaga
     2581 taatttcttg aatttttctg tcaatccagt agcaaaactt attacaaatg aattaactag
     2641 aaaaatgtat ggtagagata atttttttga aaagagttat gtaaaagtag atacacaaaa
     2701 aattgctaat atgggacttg aaaaaatatc gaaagcaagc gacctattat ttagaatagg
     2761 tgtaaattct ataaatgaca atttagaaat gcttggcaga gaaaaaataa acgagtcttg
     2821 ggctgatgaa cactatgtaa ctaaaaacta tcagtcaatt ttaaatccag atttgaaagg
     2881 aggggctaac aatggaaatg gagaaaagaa cggtgtcgct ggaaataagg gatagtaatt
     2941 tagaaagccg taagataagt ggttatgcag cagtttttag cgacgattac actaagttac
     3001 aagatagatg gggcgatacc ttttatgaaa aaatttcaag aggtgcattt ttaaaaactt
     3061 tagcagataa cactagagat aagtttatgc tcataaatca tgattggaac aaagttgtag
     3121 gtagaactaa ctcaaatcta attttagaag aagatgaaca tgggttaagg tttgaagtag
     3181 atataccagc tacaacagat ggtaatgact tacttgaaaa tgtaaggctt ggattgatta
     3241 aaggatgttc ttttggtttt aatattgtaa attctaaaac tcgatgggat gatgattgga
     3301 cattttacag agatattacg gaagttgaat tgtttgaaat tactgcaacc ccgataccag
     3361 cttacaatga cacagaaata aattgtagaa gtgatatatc tataaaggag cttagagaat
     3421 cgcaaaaaga agaaacaaag caaaatgata ataaagataa catagaaaaa agaaatgtcg
     3481 atttagtatc ggcttttttt aatgcattta atcgaggagg aaaataaaaa tggcaattaa
     3541 aaatttagat gaagcaacta atgttgaact aagagcagat ttaatggaag caataaaggg
     3601 taatgacgag gaaaaaataa caactgcgtt tttgaggatg gcggaagata tacaagaaaa
     3661 tctactaaaa gaagcgcgtt ctattgctaa tatagaatca gctgacagag caatacttga
     3721 caaaagaggg atgccgcaat taactagcga ggaaagagca tactatagta aagttataga
     3781 aaaaagagga tttaccaact tagagataac tatgccacgt actgtatttg atagagtatt
     3841 tgacgactta gaacaaaatc accctttgct tagtgccata acttttgaaa atacaacagg
     3901 agttacggag tggataataa ggaaaacgaa cgcagaagct gcatggtggg gtagattaga
     3961 tgatactatc aaaaaagagc tagagcatgg atttgataaa attaatacag aagcttataa
     4021 attatctgct tatcttccag ttgcaaaagc catgcttgat ttaggggctg aatggttaga
     4081 cagatatgta agagtagttt tgactgaatc tatatcattg gggcttgaaa tgggaatagt
     4141 agcaggaaca ggaaaagacc agccgaaagg tatgataaaa gatttaaaag gcgcagtcgt
     4201 tgaaggcgta tttccagaca aagctgcaaa accactggct gattttaaac caataacact
     4261 tggcaaagag attatggcac cacttacaaa caatggtaaa agggcagtcc caagcgttat
     4321 aatgcttgta aatccggttg attactgggc taaaattttc gctgccacaa ctatgttaac
     4381 atctaatggt acttatgtgt atggagtttt acctatccct gggaatataa ttcaatcagt
     4441 tgctgtccct aaaggtaaat taatagctgg tatggcaaaa gattatttta tgggagttgc
     4501 ttcaagcgga aaaatagagt attcagacga atataaattt ttagaggacg aaagaactta
     4561 tctagtaaaa caatatgcaa caggagcgcc aaaagacaat gattcatttt tagtatttga
     4621 tatagaaaac ttagacacaa ctttagcaac agaaattaat tcaaacacaa aaacgtcagc
     4681 tgctagagcg aaataggtga aatatatgga aaatgaaaac attaaaagtg tagttgaaga
     4741 ggtaaaagaa tatctaaata taacttggat agaagaagat gcaaaaatag aaaaaatggt
     4801 tgtaagagga attgattact ttgaaaatga aatagcagga atagcactgg attttaaaga
     4861 taattttaat agagaattgc tatttaatta ttgcagatat gtaagaaaca atgctattga
     4921 atatttcgag gaaaatttta gtaaagagct tcttagactt caaattaaaa gtgcagctaa
     4981 tgaggtaata tagatatgaa aaaaataaaa tttaataatt ttaatgatgg tattgttgag
     5041 tttggagaat acaaggaaag ttatgatata gaaggtaatg cgttagatga aaaagagttt
     5101 attgtaagtg gtaaactctt ttattctaat gaatatataa gagagcaaga caaacttaaa
     5161 tttgacgata cagggttaaa ggtatctatt aaattaaaag ttccttacat gagattaatt
     5221 aaaacaagtg atgttataaa gttaaataat gaattttaca gcatagtaaa acttgatatt
     5281 agcaatgaca aaactagtgt gtatttatac ttaactgaat tagttgacgt attagataaa
     5341 catatttcta tttttatcaa agagagaaaa aatgttcttg aggatgtaac aataaaattt
     5401 tataaaaaaa tttgggcttg tgttgaagat ttaaatagta aagaattgat agaaaataat
     5461 tcgattaaaa cttctacaaa taaaaagttt aaaattagat atattgaaga attagattta
     5521 agtataaaac aaaatgctat aactaatttt gttattaaat acaaagataa attttataat
     5581 ataactcaaa tcttaaattt agaagaagaa aataaaatac ttgaaataac agcagaatct
     5641 aagtaaaaaa aagttaggag atagtatgca ctcaaaaata gttactatac ttaaaaaatt
     5701 aaatctaggt attggttttc aagaatacga caatttttca aatgatgaag aatatataat
     5761 attttttata gaaaatgaat atgatgcaaa tttttctgat aatgaaaata aaagtattgt
     5821 atattcaata aaaatagaat attggtataa gagcttaaag aatataaaca aatatattag
     5881 tataaaagat atatttaaaa aagaaaattt tatcttttta aattctagtg attttataga
     5941 aaatgaatat tattgtaaag aattaaaatt taaatatgag gaagttgttt aaatgagtgt
     6001 ggagataaca acagaaggat ttgacgctat tttatctaaa atagaaagca tgggtaaaac
     6061 aggcgataaa ctgttaaatg aagctgtaaa agctggcggt aatgttattt tgcaagatgc
     6121 attaccaagg gtaagcaaaa gaagcggaaa gttaaaagat ggtttaaaag taagtggagt
     6181 taagaaaaaa ggtggtacta agtatgtttt agttggtatt acgaaagagg ataattcaga
     6241 aattttttat ggaaagtttt tagagtttgg agcatctgcg cataaaatac caattaaaaa
     6301 aggtaagaaa aaaggaaggg tagtaaatca tccaggaacc agttctaaac cgtttctagc
     6361 acccgcatac gaatcaaaaa aagatgaagc taaaaatgta atgaaagaaa tattaaaaag
     6421 agggttgggg ttataatgat taatataaat gatttagtaa tagaaacatt aaaaccttta
     6481 aaaatacctg tttcatttca aaggtataat ggagaagaaa aaacatatat aactttcttt
     6541 agttatttag aacaaaattt ttatgcaaat gatgaagtga taggcacaga acattatata
     6601 caaatagatt tgtttagtaa gaataaaatg agttatataa ataaagaaat tgaaagactg
     6661 cttaaaaaaa ataattttat aaaaagaagt atacacgaaa taaaagaatc tgattatagt
     6721 taccatgctg tttttagatt cttatttttg cagaaaaaaa ggaggattaa gaaatggcta
     6781 ttataggatt aagaaactat agaattgtaa agctactaac agatacagag gaaacacttg
     6841 aatacgatac taaaataaca agattaacag gtgctaaaag tgtaaaaata tcacctaagg
     6901 ttgattctgc tgaaaattat ggggacgacc agcttttaga gactgcttcg gctatgggag
     6961 ctatagaggt ggaaatcgag gtcgctgact taacattaga agaacgtgcg cttatccttg
     7021 gataccaata taaagatggt gtattgatag aggataaaaa tttcaatcct ccaaaccttg
     7081 cttttggttt tgaatccccg aagagccaaa acggaagcag aatggtttgg ttaactaaag
     7141 gtgtctgtga acctttcgaa gaagaagcaa aaacaaaaga ggataaaatc gaatttcaat
     7201 cccaaaaaat taaacttaaa tttatgccaa ggatacatga tggtagacat aaaataaccg
     7261 cagatacgga cgtcgaaaat gcacctactg atgcacaatt ttttacaaca gattttttaa
     7321 aaacaggagt aaaaccaaca acaccagcga aagcaaattt aaataaataa ggaggaaata
     7381 aaaaatggaa ataaaattaa ctataaatga agaagaaaaa atattcaaag ctccacctat
     7441 accacttaga aaattagata aagtttttac actaaccgat aaaatcgaag atggtcttaa
     7501 ctctacagaa ttattccgcg aattgcttga ttttacagtt gatatatacg gggaacaatt
     7561 ttcaaaagat gagttgctag atggttttta tccagctggt gattttataa ataaagcgtt
     7621 agaagattta tcaaaagtaa gtggaggttt tgaggaaaaa gtaaaaaact aggtaatgga
     7681 gaagatacag aaaataattc taataaagaa aataggtatc tatctccacg tgactttata
     7741 aaagatttat atagtatgtt tttatttgaa aagaattgga gtatggttga tatagataac
     7801 atggatatat tttattattt agatatactt gcgtatacga aagagaataa aaaagataat
     7861 aataatccaa acgaaaatga cgtttatatt gaccagataa gctggttata aggatgtgag
     7921 tgtatgtgta agtactgtga agttgaagaa agcgaagcag cttttaaaag aggtaaaaca
     7981 aatagaggcg agttgataac ttgtgactgc gatatagaaa tgtggattga aaaagaaagc
     8041 aatgagtatt ttttaaattc tgaatgttgg tgtggttact atagtgaaag ttttgaacct
     8101 attaactttt gccctttttg tggaagaaca ttaatttaat aattttgaaa taaaccaaga
     8161 taaaatatct tggtttattt ttttgcaagt gaggtgagga aatggcggaa gatgtaggcg
     8221 aattagttgt tcgtgttgca atggaaaaca gctcgtttca acaagggatt aataacttga
     8281 atagacaaat gaagttaata agaagtgagt ttaaaaattc tggcgctgga gtgaaaaatt
     8341 tcggaactag tcttgatggt ttgaaatcta aacaacaaat gttatctagt actatacaac
     8401 agcaaactaa agtagttgaa gcatataaaa ataaactaaa tgaaagtaaa acaactttaa
     8461 gcaacacggc acaaaagcaa gttgaactta aagaaaaagt taatcaagct aagagtgctt
     8521 acgaacagag caaagcgaca ttaggtgaaa acgcagaaga aacaaaaagg ctaaaatctg
     8581 aacttgatag tttagaacaa gaatatgcta aaaatgaaga aaaaataagg tctaactcgg
     8641 cagcggttga taattggtct attcgagtta ataatgcaga gtcgagactt tctgaaatgc
     8701 gttcagaact agatgacaca acagaaagaa taaatcaaca agaaaataaa tggaatcagt
     8761 tagcaactaa aatgactgaa ataggtaaca aatttagctc ggttggaaaa caaatgcaag
     8821 acgtaggtaa aaaaatggct atgagtatta ctgctcctgt tttagcagtc ggagcagcgg
     8881 ctagtaagct tggaatggat tttgaagcat ctatgtcaaa tgtacaagga ttaagcgggg
     8941 caacagctga cgaaatggtt cagcttgaaa aagcagctag agaagcagga gcatctacat
     9001 ccaaaacagc taaagacgcg gctgatgcgc ttgggtatat ggctttggcc ggctgggatg
     9061 cgaaaacatc aatggaagct ttgatgcctg ttttgagatt gtcggaagct ggaaatttag
     9121 accttgcaag aacctcggat ttagtaactg actctatgtc aagtctcggg cttagtgtaa
     9181 aagaattgcc aacatattta gaccaagttg ccaaaactgc tgcaagttca aacactaaca
     9241 ttgacgcact tatggaagct atgattgtgt ctggaggtac ttttaaaaat ttaaacgtac
     9301 ctctttcgga agctaacgca ctacttggaa tactcgcaaa tagaggacta aaaggcagcg
     9361 aggcaggaaa ttctttaaac tctatcatga taaatctgac aagtggagca ggtcaagctg
     9421 gtgtagctat gaaagaacta ggattaagtg cttttgatag caatggtaag ttcaaaggca
     9481 tggctaatgt tttgatggaa cttaaagaaa aaacaaaaga catgacagaa gaacaacgaa
     9541 caatgtattt gtcaatgata ggtggaaaaa cgcaaatatc tactttgcaa gctttgctat
     9601 ctggagttgg agaagaatat tcagaacttg aaaaaaaggt tggaaatagc acaggtgcat
     9661 tagataaaat ggctaagact atgcaaaata ataataaagg ttctataacg gctttaatat
     9721 cagctgtaca ggaattagga cttaaaatat atgatgtact aaaaccttct atagcgcaag
     9781 tagttagctg gtttcaaaac cttacaaata aattaaacga aatgaaacct gctacagtag
     9841 aaactatagt taaaatagca ggattagcag cagcactcgg acccgtttta cttattggtg
     9901 gaaaaatagt tagcatgata ggtggagtta taggggcttt tggtactttt tcagcggcaa
     9961 tggcagtcgc aacaaccgga gcagccgcag caactccagc tgttggtgct ttagctgcaa
    10021 ttttaggtgc attatcaagc cctgcggcta tagctgtagc tggaattact gcgctaggca
    10081 tatcagttgc gacagttgct aaagatatgt caaaagatgc tatagagcca attagccgtt
    10141 ttggtaaaag cgtatcagaa tctacaaaag aagctgtagg cgcttttatg gatttagaag
    10201 aaaaaacgac gatatcttta aatcaattaa tgtggagtgg tgagactgtt tcagagaaaa
    10261 tgaaacaatc aatagtatct aattttacag aaatgtcaaa tcaaatcatt gctaaacttc
    10321 aagagagtaa agagcaaagt atccagtctt tgcaagaaat gtttgcaact tctaaaaatt
    10381 taagtgacaa agaaaaagaa gagttaataa aaaatacaga agaagcatat tcaagcaaag
    10441 aagaaaaaat aaaaaagagc aatgaaaaag taaatcaaat aatgactaaa gctagtcaag
    10501 aacacagagc attaacaaaa gaagaatcag cagagataaa tcaaataaaa acagatatgc
    10561 taaataccgc agttgacaca atgagtaaaa gcgaagcaga gcaagctgca ataatggaaa
    10621 gaatgaaagc aaatcatgtt gatttgtcag ctaaagaagc tgcggaagta gttaaaaact
    10681 caataaagaa aaaagacgaa agtataaaag cagctaatga agaatataac gaaagattaa
    10741 aaatagccgc acagcttaga gcagagggcg gcgcagaaaa tgaaaagtta gcggataaag
    10801 tagtagaaga agcaacaaga caaaaagacg aatcaataaa taaagcaaaa gaaatgcatc
    10861 aaggagttgt tgcagaagca cagaaacaag cagaaaacca agcgagtaaa gtcgactgga
    10921 caacaggaga aataaaatct aagtggcaag ttttaaatga tacggtaaaa ggtaagatgc
    10981 aagaatggga cgacatggcg ggcaactggg tagaaggctt gaaagaaaaa atatctactg
    11041 gatgggctaa tttaaaggaa aatacagcta ttttgtggga taatataaag cttactgtaa
    11101 caactaaatg gacagagata aaagagtcta taggcttaaa gatagaagaa ataaaacaaa
    11161 atatcagcga aaaatgggag tctataaaat tattctttac aacaacgtgg gaaaatataa
    11221 aaatgatttt cgggaacgct tgggatgcta taaaacaaca agttgaagaa aaatttgcgc
    11281 cttttgttga aaggttaaaa attatatttg atggtataaa agagtttttt agaagcactt
    11341 gggaagttat aaaaaatata gtacttggtg ctgtgttatt aattttagat ttagtaacag
    11401 ggaattttga taaactaaaa actgacgcaa tacaaatttg ggaaaacata aaacaaagct
    11461 tatcggatgc gtgggaatct ataaaagaag tatttaacaa tgcgttgact gcaataagcg
    11521 atttactttc taacatgtgg gaaggtatga aaagtactgc atcaagcgta tgggaatcta
    11581 taacacaaac tttaagcagt atatgggaaa atataaagtc aacatgttct aatgcttggg
    11641 aagggctaaa aacaatttgc tcaaatactt ggacaaatat aaaaacaacc cttgtaacct
    11701 catgggaaaa catgaaaaca gctgttagta ctaaattgac tgaaattaaa aataatattg
    11761 taactgtttg gaatagtgtt attacttttt ttaaaacact tccagccaaa ttaaaacaag
    11821 ccgctattga tatgtttaca agactaagag aaggtataga ctcgaaaaaa caagatgctg
    11881 ttaatgcatc tcaaaaagtc gggactgatg ttgtaaacaa aataaaagag ttcccaagca
    11941 agttcctgca agttggtaaa gattttgtaa gcggcttagc gaatggtata tccggcttcg
    12001 cttataaagt agtagacgaa gctaaaaaac tagggcaaaa ggctgctgat gcggttagaa
    12061 aagtgttagg aatacattct ccatctcgtg ttatggctga gattggtaaa ttcgttgatg
    12121 aaggtttagc acaagggatt gctaataata gcgactcggt caaaaaagca actgaaaaga
    12181 taacaaagat tatagaagaa gaaacaaaga aagctacaaa agcatgcgaa gaagatataa
    12241 aactatttaa taaacaaata gacgaattat ctaaacaaga aaaagcagcg cttaaaaaag
    12301 taaaaggtga cgctaaagac gcattgaaag aagaatatgc agctaaaaaa gaaaaaataa
    12361 aagaagcgat agcactaaga aaagaacaaa aagaaaaaga agttgaacag ctaaaagaga
    12421 tagcaaattc agctaaagaa cagttaaata aagagttaca agacagacaa gagtttgtaa
    12481 aaaaagttga tgacttaaca aaagcaatag ttgatgcgct gaaagaaaaa tacaaacaag
    12541 aatacgaagc acagcaagcg agtataaaaa aagagttaga cgcactagat aaatggaagg
    12601 aagaaagtat agatagaatt aatagtgtct atgatgcgaa aattaaagct attgacaacg
    12661 aattagaagc ttttgaaaaa gctgaaaagg aaaaagatag acttgaacaa gacaaagaag
    12721 aattaaacaa aatagaagaa attaaaacag ctataaagtt tgaacatgat gatactaaca
    12781 aagaacagtt gcaaaaagaa ctagagaagg ttttagctga aagaaaaaaa agaatagagc
    12841 aacaagaaat agaagataaa aaagaagctt taaaaaagag aaaagaagaa ttagaagaaa
    12901 agaaaaaaga cgaaatagaa aatataaatc aaatctatga aagcgaaaag gaatattata
    12961 ataaaagact tgaagacgct aaaaagttct atgatgaaag aataaaagaa tcaaaacttc
    13021 aagcagaagc tgaaaaattg ataatggata aaaatcaaaa agaaatagtt gcgttattaa
    13081 atagttattc agatgcatat aaaaatgcag gtcaaactct aggtgaaaaa ttacttgaag
    13141 gatttaagcc tgccataaac gaaattaaaa atctaattga tagtattaca agagaaataa
    13201 ataatgctag aaatgaagca ttgagtttaa atagcgatat tagaaaagga aattcagcag
    13261 ctaattcaaa ttcgggaaga agagaagcaa cttataataa taattcaaga acagcgaatt
    13321 ataatgtaaa cgttaatagt acagactcaa acacagcaag agaaatcgaa agaacaataa
    13381 gaagtctagc atttagttta taaaaaaata gtttataaaa aaggggtgtg aaacttgcaa
    13441 aaattaatat gtagaaactc gaaagggcaa gaagttaaat taggaaactc acgccctttt
    13501 ttgttagaaa aaatagaaaa cggagcaaat actagtgcta atatttcagt ctcgaacaat
    13561 ctaggtgatg gtgtaagtat agatgatata agcataaaag agagattact tcctatttca
    13621 gcagctattt atgcaaaaaa taaagaagat ttagaattaa aacgtgcata tttaacctct
    13681 ttgtttaatc ctaaattagg agaaatggaa tttatatata caaataacgc attaactaaa
    13741 cgtgtcaaag gagttgtgca agacataaca tttcaaacac cagttggatt gatgcaaaag
    13801 tttcttatac agctcttaac tccaagccca ttttggatgg atgagtttgt tttaaaagaa
    13861 gaagtcgcct tatgggttgg tgacttcgaa tttactttag aaataagtaa tgaaggaata
    13921 gaaatgggtc atagggtaag taatttaatt tgtaatgtag tgaataacgg agacgttgaa
    13981 tgtggcatga aaatacaatt taaagcactt gcaacagttg ataatcctag cctatttaat
    14041 gtaaacacaa gagaatatat taagataaac agaacccttg aagctggtga cttattagaa
    14101 ataacaacag aatttgcaaa taaaagaata gagttagtaa aatcaaacta tgaaagaata
    14161 aatgtattta attggattga cttggatagc gaatttttac aacttgaagt tggagacaat
    14221 ttatttagat atgacgcaga cgtaggaatt gataatttag aaatgtctat atattataat
    14281 ccactttatt tgggggtgta atatgcagca gagcataaga atactagata aaaatataaa
    14341 tttaatagct gaaatagata attacgaaga attacagatt attaaaaaat tccacaaagt
    14401 aggagagttt agtttaaaaa taaatgctaa taaaaatcat gtagataaat tagttaaaaa
    14461 taatataatt ctacttggta aaaattataa taaagtttgt ttgatacttc atcgagagtt
    14521 catgtacacc gaaacaggag aaaaaagcga cattttatct gttaaaggcg tggatttaaa
    14581 aggcttgtta aatagaaggc tcatcatacc cgacactgga gaagctttcg aaagtcacga
    14641 agggacacaa gaagaaatta taaaagcttt tgttaataat aactgcgtag acccttcaaa
    14701 ttcaaaacgt gtaattgata atcttatcat ttcagaaaac aaaaatcttg gaagtgctga
    14761 tatgtggcga agttcttacg aaaatttaag tgataaaatt caagaaatat cagaattttg
    14821 tgggcttggt tgggaggtag tgttggatgc taataaaaag aaatttattt ttgatgtaat
    14881 aactggtaaa gatttgacta ttaatcaaac agaaaacccg ccgattattt ttagaagcga
    14941 ttttaataac ataagagcta gacactttac agaaagtata attaactcta aaaatgttgt
    15001 gtatgctgga gcaagagaag acgcgacaaa actggtaatt tcaactggtg aggttgaagg
    15061 ttttgagaga aacgaagtct ttgcagatgt aagtgaaaat actataagca tactacaaaa
    15121 agaaggagaa attaaattaa aagaatatga agagttaaaa agttttgaat tagaaataga
    15181 ccctaaaaat acttttatgt atgaaaaaga ctacaaacta ggggatattg ttacagttca
    15241 agatagaaaa ttgaaagtaa caatggatac tagaattgta gaagtacaag aaagctatag
    15301 taaaaatggg ataaaattaa aaattacgtt tgggagcagc ataccaacct tattttcaaa
    15361 aataaaaagg atggtgaaat aatggaaaaa agttttgtat ttaatagtgt aaatggcgat
    15421 aggagataca aggcagagga ctttagagaa tattttgcta gttttatagg taatggtgta
    15481 ttccctaatc caagtagcaa tttgcaagtt gtagataata ataatatgac tataacagta
    15541 aaaaaaggca agggctggat taacggagct atctatataa acacagacga tttagtaatt
    15601 aatatagacc ccgcagatgg aattttaaat agaatagaca gagtagtttt aagatttgat
    15661 actttaaata gaaatattaa attagctgtt aaaaaaagtg cttttaatag ttccccagct
    15721 gcaacagaat tacaacgtga tgcagatgcg tacgaacttg gattagctga tatttatatc
    15781 agagctggag caataagtat tacacaaagt tctataacag atttaagatt agataaaaat
    15841 ttgtgtggaa ttgtaaaagg tacgatagaa gaaatagaca caacaacatt acttgcacaa
    15901 ctaagtactt ggaaagatga agaaataagt aatttcaatg attggcggga aaatcaagag
    15961 aataaacaag aagattggta taacactaca acaacaaatt ttgtatcaga gtttaacatt
    16021 tggtttgaaa gtataaaaga tattttggat gaggatgcag ctggaaactt attaaatgaa
    16081 ataaacaaag taaaagaaga gttagcaaaa atagaaacaa cagcagagaa aacaagctat
    16141 aataatacaa caagtaatct tactgctaca aatgttcaag gggcaataga tgaagttgtt
    16201 agaaaaatag aaaaatttaa tgaggttaat atatctatac aaaacgatat gttacctatt
    16261 taagagagga gagtgaaaaa tggctatagt ttatgaattt aattatacag gtgctgaaca
    16321 aagtgtagtg ttgccacctg gtaaatataa gtttgaatgt tttggtgctt gtggtggtaa
    16381 ttattatgat tttgtacagt gtgcaagagg tggctatact gctggttctt taatattgaa
    16441 agaaaatact acattacatg tttacgttgg gcaaagtggt tattgtaaag gtgttaatgg
    16501 tattgagact tgtagaagtg gttttaatgg tgcgggtggt ataaccactt ataaaagtac
    16561 ttctgatggt tattatagtc ttgcaggtgg tggagctact gatattagac ttattggtgg
    16621 taactgggat aatttacaaa gtttgctatc tcgcataatt gttgcaggtg gtggtggtgg
    16681 tggaagcgga aattcacatg atagtattgg tcatggtggt ggtacaaaag gtaaagatgg
    16741 tatttctata gcaaataaat attttgcagg cggtggaagc caatttcaag gcggtttaac
    16801 ttttaatagt ctttataatg gttcttttgg tgtaagcggt gctggagatg gcattagtgg
    16861 tgttggtggc ggtggtggtt ggtattgtgg tgcaggtagt ttttatgctg aatttggtgg
    16921 tggtggaagt ggatatattc taacaaaaga tagttataag ccagcaaatt atagtccatc
    16981 ttctaaatat tattttagcg atataaatag cgttgtaggt ggaaatacta cgaaacaaga
    17041 tggttatgct aaaataacat tactacaagc attgccattt ttaactatat cctcatataa
    17101 ctcaaccaca gcaacattta aagctgacca cactgacact actttattga ctaagataga
    17161 atattttata gatgatgtac taaaagaaac tataacaaca gatttaacag cagaaaaaac
    17221 aattaactat acagtagaag ataatgcact acacgcactt aaaatagttg ttacagacag
    17281 tgctaatgct acagcagaaa aagttgtaag tataagcaaa ggaattgcac ctcttcctgc
    17341 tggttctaca acagatgaag ttacaagcaa atggatggaa attaaagata cattcaaaag
    17401 tggtaaaaca agtattataa acactttggc actaaagaat atagaagcaa gtttaaataa
    17461 cacattagtc gagttgtcag agaaaataaa aacaagtttt gatagttcag acgctagtgt
    17521 gcaagatttg atgaatcagc taacacaagc taataatact ataagtcagt taaattctaa
    17581 atataaagtt gctagtggca atagtgttgt taattattgt actaatacaa aaggatttta
    17641 ttttaatagt gactatctgt tcctatttcc tggagccatt caaattaacg gacttaattt
    17701 tgttcctaat atatttttta ctacttttga attaattgat gatggttatt ttcataaata
    17761 ttttgttttt gcttgtcgtg gtattttcac tcaagatttt gtaattactg ctcattatta
    17821 tcgtctaaaa tcatatttac aagattttaa agttggaggt gaggttctta aattaaatga
    17881 acgtgatgtt tatatggata atagaggtat acaacttcct tgttcaagac aaggttcttc
    17941 ttttaaatgg caagctataa aatttattta ataaatgagg tgataaaatg aatagagcaa
    18001 atagaattat atgtgaccaa acaggtaaaa tacttttgca gacaggagaa gcaacaggag
    18061 atatactaga acatgataca ataacagaat tacattatat tgatgttgag tatggaagta
    18121 tagactatac aagaaataga attataggta taaatataga aacaaaagaa ccaattttag
    18181 aagaaatacc agtatttatc tcggaggaag aaaaaagaat acaagagtta gaaaatcaaa
    18241 ttttattaaa tgaaaatgaa aaggtaggag gaattttata atgaatataa ataatgttgt
    18301 agtaagaata ttagcagaga gaatattaaa cggaggctta aaccctttaa aaaatcgaga
    18361 atttgaactg gatgatgtaa ctaatgcaga atacagaaaa gcagtagagg attatattat
    18421 agagcatagt ggagtagtag aaggaacaca acctacaaaa taggttcttt tttataggct
    18481 tagataattc taagtcttat tttaatgcaa aataaggagg aaatatgaac gtaacaatag
    18541 tttttttagc aacaaatata tttataaaat tagtaatatt agcaatagca tttgatacat
    18601 tgctaggttg tttaagagca ataaaaacac atcagtttaa ttcaagtttt ggaataaatg
    18661 gaggaataag aaaagttgca atgatagcat gtatattttt tctagcagta gttgacattc
    18721 ttacaaagtt taacttttta tttatgttac cacaagattg ggttgatttt ttgcgattaa
    18781 atcatcttgg aatatctgaa tttttctcta ttttatttat tctatatgaa agtgtaagta
    18841 tattaaaaaa catgtatttg tgtggattac cagtgcctaa acgattaaaa gaaaaaatcg
    18901 gtagtttatt agatactatg acagatgaat taaatgttaa aggaggtagt aaataatgaa
    18961 aatatgtata acagtaggac acagcatttt aaaaagcggg gcatgtactt ctgctaatgg
    19021 agtagttaac gagtatcaat acaataaatc tcttgcacca gtattagcag atacttttag
    19081 aaaagaaggt cataaggcag atgtaataat atgccctgaa aagcagttta aaactaaagc
    19141 agaagaaaag atttataaaa tacctagagt taatagtgga ggatatgact tacttataga
    19201 gttgcattta aattcaagtg gtgtaggagc ctttggaaca gaagtatttt actatagtga
    19261 aaaagggaag gaatatgcgc aaagggtagt agataaatta tctaaacctt tcataagaaa
    19321 aaaaggagat aaagaagtag gtaatagagg tgttaaatta gataaaagtt tatatatttt
    19381 aaatagttct aaacctactg caatactaat tgaaagtttc ttttgcgata acaaagaaga
    19441 ttatgagaaa gctaagaaac taggtcatga aggtattgct aagttgattg tagaaggtgt
    19501 attaaataaa aatataaata atgagggagt taaacagatg tacaaacata caatcattta
    19561 tgatggagaa gttgacaaaa tccctgcaac tgttgttgga tggggttata atgatgggaa
    19621 aatattaata tgtgatataa aagattatgt acctggccag acacaaaatc tttatgttgt
    19681 tggtggtgga gcatgtgaaa agataagttc tataactaaa gaacattata caatgataaa
    19741 aggtaatgat agatttgata cacttcacaa agtattagat tttattaaga aataagtttg
    19801 taaagagtgg taactatttc atcagttacc actctctctt tttttatcct ttttgttgta
    19861 ttctctttca agtgctgttt ttttaaaaac ccatgtcgtt ccgaattttt tacagtctat
    19921 accttccaca aattttttgt ttcttatatt aagtcttaac gtactttcac cctttttata
    19981 aagttcgcaa gcatctttta tagagattag ttcttcaaat atatctttca ttttaacaac
    20041 tccttttact tatattataa tcgcaaaagt ttaaaaagac aagaaaaaaa taaacgttta
    20101 tgtttaaaaa actattgact taataaacgt aagcgtttat aatgtaatta aagaaagaga
    20161 taaataaaag ggggatttaa aaaatggaaa aattcatcag acttgatttt gataaaggtt
    20221 ttagaggtaa agaacataga agtagcgcaa caggagatgg agaacatttt gaagcaggaa
    20281 ttagttgcta taaaataaat aaagaaaaat gtgttgatgc tataataaat ttatgtgaat
    20341 attggtttga atttgcaggt gaatgtcaat tcaaagattt cgatataaat atttttgaag
    20401 ggtactatgt aggtgaaggg gctagttatg aagatttagc aacttgtgaa aatcacttac
    20461 attgtgtaga tggttctttg tttaatgaag tttatgactt atattatatg catgaaacat
    20521 acttagaaga aaatagagat attgaagaat tagaagaaaa ctacaaagat gaatatataa
    20581 caacagaaga atttgaaact aagataaaag aaatgtttat aaaatacctg taggaagtga
    20641 aaacatgaaa attggagata aatttgaaaa tcttacaata ctagatatag aacaaagaaa
    20701 cagtagaaaa tattgtctat gtaaatgcgg gaattgtgga aatgaaaagt ggataagagc
    20761 agatagtttg aaaagaataa aagcatgtgg gtgcatgaag agcaacacac aatttaaaca
    20821 aaatgattta acaggcaaaa agtttggcag attaacagca ataaagaaca ctaacaaaaa
    20881 agctaaaagt ggtcattata tttggatttg taagtgtgct tgtggaaatg agattaagac
    20941 aacagaaaac aatttaacta ctggtagaac taaatcatgt ggatgcttga aaaaggaatc
    21001 taatataaaa aatgcaaaga tagcattaaa agtacataaa gaaaaaaata ttattgatga
    21061 tacaaatcta tctattataa aaaagacaga agcatatcct aattcaaaaa ctaaaattag
    21121 aggagtttat tataataaag aaaaaagaaa atattgcgca cagatagaat ttaagaaaac
    21181 acattataat ctaggttact acgataacat agaagatgct gaaaaggcat atatagaagc
    21241 taaagataat ttacataaaa aatttttaga aaaattgcat gaataataat aataaatata
    21301 tttgatagta gaggaggaaa ttaaaatgaa taaacaaaag gctaaaagat ttctaagagc
    21361 tatagatatg aatatagata aaatagagga agaagctacg aaaatttata aagaacgcta
    21421 cttaatagaa acaactgatg ctataagaat ttctataaat ttggaaggag ttgtaaaaag
    21481 cacaatttct agttggcaag gctattcaga tgatattttt aatatgagag aagttattgt
    21541 ttatgaattt agtcaagaaa aagttcaaat tgatgatttt ttaggagaac tttgcttttt
    21601 aaatgattac gaagaatttg ctacttggtg tgacactgaa agcgagactt taaattggaa
    21661 cagttatgaa aagttcaaca aagaaaattt tgacgaatta gttgaaagaa atatagaaga
    21721 tggtttacca atttttttga aagagttaag tgaaagtata gaaaattgta aacaagattt
    21781 aaaattaatg attgaaattt aaatgaaaga gtattattat taatgtatat agtatcgctt
    21841 ttaagattaa ctacgaggga tttaaaatat tttttaattt atttagaaat gatattgtaa
    21901 taagaatttt tatattaact ataagggatt taaatatcaa tttaatttat atcgaaatga
    21961 tacaatatcc aaattatttt agattaacta tgcggaaata aaaacagtgt attcaataaa
    22021 tacactgctt ttttaatgct aaagatttag ttattatgat aacttaatcc ttcgattata
    22081 aatcctacgt tcgtcgtcat ttccaattcg ctactttgca atattccttc tgtaaattcg
    22141 ctgtcatttg gtaatgatac tttttttctt gcaactgctt ctatgattgt ttgaaatagt
    22201 ttaattttca tatcattatc aagtgcagcg aatcgcaaat aatttgttac ttcttgtgaa
    22261 aacttattta attcagattt agtcattttt tcagataact cttttccaat ttgcaaataa
    22321 acattcatct tttccaaccc ctttttttat ttataataac actatagtat tgtttctata
    22381 atgctgcaac aatatatcga tattattaat aaattatttt aacattctac ataaaactat
    22441 tttattattc ttatataaat tattataaaa ttatcataga attttataca aattaaataa
    22501 ttcttcttat acaaagtatc ataagattct atataaatta ttataaaatt attttactat
    22561 ttttcttcct cttccttttt ttcttttatc ataagacttt gccaactttt caatatcttc
    22621 actatcttct aaatctattc tagttgtatc tataacaggt tttaaaacat ctaagactaa
    22681 cttattaata gtagtctttt taatctgcgc aactgctttt aaaacgttta tatcatcact
    22741 ttcaaaatat atagatatag attctttttt cacttcttca ttctctttta cttcaagtaa
    22801 agaagtaact atactgttag aatttttttt aaaagtatct tgtattcttt tctctgtaat
    22861 atctctataa tcaaccttat tttcattttt ttctgaatca aatttcattt tataatattc
    22921 ccctttcgaa aagttcatct atatagtttt taaattcttc tcttacttta tgcgcttttt
    22981 taattttctt acaatattct tctatatctg ttttatttaa aattgcattt ttaacaattg
    23041 taccttcgct tattcgtgta tttaataaaa ccttttttat gttatcatat ttttccaatt
    23101 gattattaaa ctcatctcct gtgctaagtt ttctgcttgt atatctattt acaactatag
    23161 ctttttttac attgttgtct atttctagtt tctctaagtc ttctttaaac ttagtataaa
    23221 aaagattgca tcctcgaata gaagctatat cttgaaattc taaaggggta acgatactat
    23281 cagaaattaa taaaaaatta cgtgtaacaa catcataact aggtgacaaa tctattaaaa
    23341 tataatcgta ttttccgagt gttcctatgt tcttagcgaa ccatctgtaa actgcctttt
    23401 ctctatttgt ttcagttact aataattccc ccaacacgca acattcaagt ttacttccta
    23461 ttaaatctat gctaggaaaa tcttttagtg gagattttat tataatatct tccgctttag
    23521 ttttataagc taatgcttca aacattgtat ttccttcgaa caatgtgtta tgtataaact
    23581 gtgtagtatt cgcttgaaaa tctccatcta cgataagaac ttttttattc aatttagata
    23641 aatacattgc tgttaaaatt gttaaagtag ttttaccgac tccacccttt acgttaaaaa
    23701 tagaaattgt gttaagactt gtactcaatt aaattacccc cttttttatt tttatacaat
    23761 actattatat catatattcc gaacattttc ggatatttaa taatttattt taatttaata
    23821 ttaatcttta tataattaat taattacata aagattattt ttataaaaat taattaatta
    23881 tttttatatt aatttatata taattttata ttattttttt aataatcaat aaagaaggct
    23941 aagaaaaatc aaccttcttt aaaaagtcat tcattcttag ccttttcaat tattatcctg
    24001 tcgccctcga atgttgccgt aacattaggg ttttctcttg ttatgcccat ttcatcagcc
    24061 cattttttag gaacactcat tctaggagta aacgacccac tcccaccttt atgaaagtta
    24121 aactttaaat ctcttttttc ttttttcatt cattagctcc ttttttgctg tattacaaag
    24181 aaaagcaata atacaacact aaaaactaaa ccgagcatgt tgcttgtact agagtttaat
    24241 actatagata ctatatttaa gactatagtt atcaaaacta aaacaatgca aatcttataa
    24301 aatttatcac tcatgttttt tagaaatgtg ttataatatt agaaagaagt cacctagaga
    24361 gaagggctat tccctctagg cttatcctta cagttctttt aaaattctta gaactgcgag
    24421 gattatgtta agtaataata ggatgaaaga gaaaactttt ttaactatgt taaatttgtt
    24481 ttctcttttc tttttacttc tttctaatct ccccattccc gttcctcctt tcttttattt
    24541 tttttgcttt ctcacctcct tctctataaa tatattatat catgacgggg cgtcaaaagc
    24601 aatagttttc tcctattttt aaacaaattt tggtaaaaag taaagatttt agtatattag
    24661 aaatgatata aattcgtatc ttttaaagaa aatgtgatat aataaaagca agagaactgt
    24721 atttcagtca taagagtaga gttttcgtct aaagtaaaaa attatctaga ctttttgaat
    24781 ttgattttta aatcaaattc ccaaccactc ttttgcgcag agtggttttg tatttttaaa
    24841 ctaattaaca tatatgaagt gatgtaaaat tgtatctttt ttaaaaaatg tgatataata
    24901 aaagcaagga aataatgatt tactttacac aagagtagct atttccaatg ttttgaaaaa
    24961 taacttattt tcttgaacca ctcttattgg cgtttgagtg gttttttatt ttatcatata
    25021 tggaagctga tataacacca gctagtatac tcaataaaaa atttatcacc atattgttcc
    25081 acctccttcc gttatggaat ttggcgttta aatatggaaa tagccactct tacgcttttt
    25141 caatcacttt tttcctcgct aaaattatta taacatatat ttacataaaa aaagacttag
    25201 taagtgtact aagtctttta aaatataatt tattttcaaa atcaatatat ttttttattt
    25261 tatatttatg ctataattaa tattaactta ttacagatta caaaaacaaa taaaaggaat
    25321 aagcgtttgt ttggcgactc gacttattcc ttaccaacgg agcttgaagc tcttatagga
    25381 tataattata tcctttttta actaatttat aagttatatt atagattaaa acaacttata
    25441 aatcaatgtt ttaaggttaa aaataatttg aaatttttaa cttaattagt taaaagtgct
    25501 tcaagccccg tcaaaagggg gaaataaaat gtctttagaa gaagcaaaag aattagaaaa
    25561 aaattataaa ttaccaatgc ctacgtattg tttaaatgaa aaaaattatt gtgctaaaga
    25621 gtttggcgct ttaatgctta taagtaaagg gagcatgaga attatgcata tagaaaaaaa
    25681 tagatatttg tatcaaaata aatttaaaat ggaagaatta gctaaacaaa taggggttgc
    25741 tcgtactact ttagagagaa atataaaaaa gctcaatagt ttagattgta aagtacttga
    25801 aatagaaaac agccgcaacg gaataattta tagattaaac tatggtacaa gtacaggata
    25861 caacgataac gttcataaat ttgttacaat tcatcatgat atgctacaag aattaatttc
    25921 tgcatttaat actaatgcaa taaaagttta ttgcctttta tgttacatga caactgaaaa
    25981 tagttttaaa tgtatgactg aaaaatttat ttgtgaaaaa atcgggttat gtggtgatag
    26041 caaaaataac agaagtaaaa taagaaaaat tataacagta tttgaagtaa gcaaatatat
    26101 cgaagtaaaa aaagaaaata aatttgagtg ggacgaagaa aagaataaaa aagttcctag
    26161 gatacaaaaa ttatatagac tatgtagttt ttcagaatgg aaaaatgcac gtaaaaaata
    26221 gaccttcttt aaaaaaggtc tatttttatg tctaaatata acaatgctgt ttataacttt
    26281 gatttttatg ttgataacca ttatttttat gttgataaca tgttaataag tttttaaaaa
    26341 ttaattagat ttcttactac gtgcaaaaag tggtgctttg gtagtgcaaa aagtggtgct
    26401 ttggtagtgc aaaaagtggt gctttggtag tgcaaaaagt ggtgcagtaa atatatagag
    26461 aatatattat tatagagaac ttgtattata gagaacaaaa tatgtatctt ttgtatttat
    26521 ttattaaagt acttaactta attagaaatc taacttgtct caaaaagtgg tggtcgctat
    26581 ttaaaatatt attagcattt ttcaaaagta aagaaactat tagaatgtat ctttggataa
    26641 aaagaaaatg attatttgat taagaaacta gaatatcatt tagaagcgat acaagacacc
    26701 agaaacgatt tttctgtttg ttataaataa tttatgttaa atagcataaa aaaatgctta
    26761 gaatggaaat aaataggtat tattctttta aattattttc atactactag aatagaaaat
    26821 taaaggaata taagttttga tgttgaaata tataacaaaa cataaaaaca tggaggaatc
    26881 gaggatgaaa aaagtattat tattaattac agtatgttta ttaagtgtag ggttggttgc
    26941 ttgttcatca actaataaca aagatactgg aaacacaact aaaaaagaaa agaaagcaaa
    27001 tgaaaatgag gttgatggaa ttaaaataac tgtaaaagat gttacaaaag aagatataaa
    27061 aggagatata aacgaagacg gaagttttaa cgaaaatgga gaatatttta gtgatggaat
    27121 cgaaaagaaa aaggcagctg attatgtgta cgaagttgta aatgtagaag tagaaaataa
    27181 aacagataaa gcagttaaat tatttcaaac aggatggaac gcagtcggta cagatggtta
    27241 cgaatttaaa gacataaaag taactgataa attagacaat caacaagtac cagctaacta
    27301 caaatttgat gcacaagtta aaatattagt tgaaaagaat atgaatgtaa aagaaatagt
    27361 tttaaaatat aacctgaaag attattcaag actttttgaa gcaatggagt acgcaaatca
    27421 aggagcatca aaatcagatg tagaaaagaa atttccggaa ttatataaag ataactggat
    27481 tgagctagga gaaattaaag tagagcagta aaaaaggggc taggtataaa taattaccta
    27541 gtccttattt ttatattctt ttaaaaaatc cctgctaagt gttttaactg cttttttatt
    27601 atatttttta gtatttttta aatgctccct ttctttttcc aaaaaaaatt cagataaaat
    27661 caattggtca atttctaata tatcagctaa taaaattatt tgagttaagg ttgggctgtg
    27721 tttaaatttt ttgttttcca tccttgaaat gtaactttga cttattcctg tcaattcact
    27781 taatcgacgt tgagtgagtt ttttcttttt tctatgatat tttaataaag actccatgtc
    27841 gaaattaaac tatagtatta aaaaatatta aaatcaatag aaatttttta caatttagaa
    27901 aatagacaaa ttaattggta aattttgtaa attaaagtcg gaatattcgt cgtacgaata
    27961 tttttggaat ggttttccgt gttaatatta gagtgtagaa caaagctgaa tacaaaatca
    28021 tgcagttttt aaagggtttt aagaatttaa ttaagagtat ttaaaatagg aggaagaatt
    28081 atggaagaga taaagcaaga gataatagag gttataaaaa aattagacat tcataatgac
    28141 aaaagaagtt tgctaatgat aaaaaattac ataataagtt ttttaaaaag atagaagaat
    28201 agcgtacgct attcttctat ctctagtaag gagtcaatca ttttatcaaa taccgttaaa
    28261 tactttttat ttaatttaaa cattttcaaa ataatttctc tctcaacttc attttctttt
    28321 ttgcttaatt ctccaaccaa agaagcgtat tgttcgtcca aatctttaat agtaaacatt
    28381 tctcctgtac catttttaag ccattcttct gaaacattcc attttttaca aatatcattt
    28441 aaagttcttt cgcttgtatt tctcttgcca ttttcaatta aagaaatagt atttcttttt
    28501 aaagttaact catttgcaaa ctcatcttga cttaaattca attttaatct taattctcga
    28561 atccttttat ctatcattgt tatacctcca tatggttatt ataatttaaa tatgcttaca
    28621 agtcaacaaa taaaaataaa aaaagtatta caaagtagtt gacttgtaag catatttaaa
    28681 ttataattat attacagaat caacaaaaag ggggtgaata aattggtaaa cataaacgaa
    28741 atgagagaag aaatatacag aaagatagaa ctccttgacc ctactaaact taaattagca
    28801 ctgaatagag tgaatatatt aaaagaaata caagacatag aagaagaaaa tattcaaaac
    28861 taaaggagtt gtaaaatgaa tatctctaat tttaataaat atcaaaaagg tgaaattgtt
    28921 caattatacg aaacaaaatt cagttgcaat agaaattgca ttgttacata tgcagatgaa
    28981 aaagaattag tattgatgta ctacgacaaa gaaatagaag aattaatata taaaacttta
    29041 actaaagaag atttgatatt taatgactat gaacttaagt tactttctta ataaataatt
    29101 gttagagagg ggtgatttat ttgaggttta gtatacatgg ttttgaccaa aaaacagctg
    29161 ttgaatttgg attaactaat gatgaacttt tgattttaag gcatttcgag gattttgttc
    29221 acagtgggaa aatggattct ttttatgaag aaggatatat gtattattgg gttaattata
    29281 ataaattcct tgaagatttg ccaatattaa aaatatcaaa agatagatta ggagatataa
    29341 tgcttcatgt tttaggagaa aaaccaatag agctagagga aaaaatgaaa tgttattctg
    29401 aaaaaatgtt aaaaaaagtt aaaaatagaa aatatattgg tcttttaaaa aataaaacaa
    29461 taagaaatcc agttgcagga gttagaagtt attttgcatt tactcttaag ttttataagt
    29521 taaaacaaaa aataactaac aatgacgata gtataaacac cgattcggta tcaataccgg
    29581 aacgattcgg tatcaatacc gatatagatt cggtatcaat accggaacct aatcggtatc
    29641 aataccggaa caaagattat tctattaatg attattctat taatgatatt gatagatata
    29701 gtcctttaaa taaacaagag gtacagcaca caatgcctaa cgttaggaat gaggtgctta
    29761 accctacggc atgtggtgct gtacatgtta ggaatgaggt gccgagtaac aagactaata
    29821 ttaacaagac taatttaact aataatatat atagttctgt agagcaaaaa gaagaaaaga
    29881 caaaagacaa agaaatatat aaaagaatag ttgattactt aaataaaaca gcagataaat
    29941 cttttaagag tacaacaaag aaaactatat ctttgattga tgcaagactc aaagaaggct
    30001 ttacagaaga agaattttac aaggtcatag ataacaaagt gttaaattgg ctagatgatg
    30061 ataaaatgaa cgcttattta cgccctgaga cgttgtttgg aaacaaattc gagtcttact
    30121 taaacgagac acctaaaaag tctctaaaag gcaatgagag cgtcaaagga aaaacaaaga
    30181 caaataataa taaatttgct aatttcaatc aaacatttga taaatattca gaagatgaac
    30241 ttgatgcaat tataaagaaa agtcaaagtg ctaaatttaa ataaaattaa aaaaggtggg
    30301 gtaaaaaaat ggaaattaat agcgtaagag agttaagaga attgtttgaa ctttttacag
    30361 ttataaaagc ggaaggcaac aatgttgttt cttgcaatga aactgtaaac gagttaagta
    30421 acaaagagct aataaaagca aatgaaaaca aatttaaaaa gccttggaat gatgctctat
    30481 tatatgaaat aaatgcttta gatataataa aaaagcataa gcacagtgat agaaaagaaa
    30541 aggcatataa aacttatcta ggattagaaa atgcagatat agaagaaatt tataaaattc
    30601 attatggaga taaagaaaat tctgaaactg ttgcaagttg cgaagaaatt agttcagcaa
    30661 aaacggatac aatatcttgc aatcatgaca gtgtttcaaa aggtaaagat actagtgatt
    30721 cagaatgtac ttgttttatt tgttctaaaa aaatagaaaa tcaagaagat gtatttaaaa
    30781 tacctttttt aacacgagat tatttgagtt cagcaaaaga aataaaaaat attgaaattt
    30841 gtgagaaatg tttggatggt aattcgtgga gtattataag aagaaagcag tttaagaaaa
    30901 tgaagaaata aatatttaaa ttagtatctt tacatgtgaa aaaggagagt gaaaaaatga
    30961 taaaaaataa tgttttaagt tatgaagaaa aaataaaagt attaaaaaaa catggttata
    31021 gtgattttac caaagaatat gttttaaaag aatttgacaa ggaacatgca ttttacaaat
    31081 catttttaag agcaatagaa aaagaagatg atggaaaagg tgtgttaatt aaaaaaattg
    31141 gaaaaggtca gcatcaattt tcaaaaggtt ttataaaaac acacactatc agtgagttat
    31201 tggaagaagc aaaacgtatt agaaaaatat tagaataagg cttagaaatg tctataatct
    31261 aaaccttata cttaaattaa aatttaccta atatgtactc tattgcgata ggtagggaca
    31321 tatttaaaaa acttgaagtg gaacttttta ttttattcca tttagtgtta tctcgtattt
    31381 tatctaaata ttgataacct tttaaagaca actgcatgac ctctaaaaca gtatcatcac
    31441 cgtaattaac atcgtcaacg agattatttt gataaattaa atttatgtga tgaacaatag
    31501 ttgacttatc gtatgtgaga ttatctagta tagtatcaaa ttgtaataca ggcgaaatgg
    31561 gagttgtatt agattcaata agtaataaaa tatctcttat acaatcttgg ttagtaacca
    31621 tagtatcacc tccttttgct ttagtatagc acaggagaaa cgagacaata ataaatttta
    31681 ctgtaacaga acgagaagga gcaaagcaaa tgcaaaagag aattacagaa atgagtatat
    31741 tagaaaaagt tgatttaatt ggacagaaac aaaagaaact acaagaaaaa tataaaaatc
    31801 taagtaaagg agaattgttt tctattgctt gtaatgaagt taaaaaaata gaagagcagg
    31861 agggaaaata ttttgaatat aggtagttat acattagtta agaaacaact agttaaagag
    31921 ttagaagaag aaaataaaca aaaagaagaa tgtttaaaag aacgtgaaaa acacataaaa
    31981 gaattagaag agagcttaga atattttgcg agggatgcgt tagaatcaga agaatgcata
    32041 atagatattg aaaatatttt gatagaacaa gctgtagaat tagaagctta taatttattc
    32101 atagattgca ttttaaacaa agatataaga aaagctattg cgatttataa cagaactaaa
    32161 agcatcagaa ttaagcataa aaatattaat tctatatcta agaaaattga aaaaataaaa
    32221 caaataattt ccttgtagaa ataggagagg tttaataatg gataagttcg taacatgcgc
    32281 tagatgcgaa agaacaataa atgtagaaga aaataattat gtgaagtatg aagaagaaac
    32341 tttaaattta agttttacgt tgtatttctg tttaggctgc gtagataaat taattgaaga
    32401 acaattaaaa tcaatggagg gagaaaatga ataagattag tgttacacaa gcattagaaa
    32461 aatttgatag tttactagat aattggaacg accttccgaa tcatgtttac aaaaaagaat
    32521 atagaggtaa attttatgac tggataaagt cacttgaaag aaaagatagt ttacaaaatt
    32581 ataagatagt tgaagtttta aataatgaac gaaatggaga agaagcgccg ttttggaatt
    32641 gaagaaatat aacttgaaaa ggacttaaac ttttgttaga tagaagggat gtatataaat
    32701 atgaaaatga taaatgtttt taaattatca tcaatgtatt gtatttgccc caaatgcggg
    32761 agcgataaac ttggaaaaaa tgaaggaaag ttaattgttg atgaacatac ttattacagg
    32821 gaatgcaaat gcggttttaa tgttcttgta gatgaaagag aagacgaaat ataaggaagg
    32881 tgctggaaat gaatagtgtt attttaattg gaagattaac aaaagaccca gaacttaaat
    32941 atataccggg ttctggaaca gctgtatcta cttttacaat agcaatagat agagattata
    33001 tcaaaaaaga tggaactaaa gaaactgatt ttatacctat tgaggtgatg gggaagttag
    33061 cagaagtatg tgctaataat ttaggtaaag gtaggttagt agcggttgaa gggtctataa
    33121 gagttaatag ttatgaaaaa gacggggaga aaagaactta tacaaaagtt catgctaata
    33181 agattaagtt tttagactat aaaaaagatg atactgaaaa agaatatatg tttgaaccga
    33241 aaggtttaga gcaacaagga tttcaagcta tagatgaccc agatatacca ttttaaagtg
    33301 ggagggataa caatgtctaa tacaaataaa aaaacagcta aaatttgcgg atggtgtgga
    33361 acaacaattt attcttatat ggatggtaaa attgcatatt gttctaagaa atgtaagaaa
    33421 aaaagagaaa aaaatttaga aacagaaggg gcaaaaaaat gaaagagtat agaattgaaa
    33481 ttggagaaat gatattttta aaacataaag ttattgttga agttccagaa caaatatgcg
    33541 cttttgatat atataaatat atagaaaatg aatgccaaaa caaaagtgaa atatctgagt
    33601 atatagaaaa aattggtggc aaacaattaa attttataga aggtgacagc ggagatagcg
    33661 acttaacagt tgaaagttgt aagagaataa aaaaaggagg aaaaaagaat ggaaaataat
    33721 ttttggtgga gttttatgga tttagaagaa gcggaaaaaa gttgttacag aagaaagtat
    33781 aaaagactta gaaaatgcta taaatgaaat tgagaatgaa gcggaagaag ttaaaatttt
    33841 aattattaag aaaaatggcg atgaaaaata tattaatttc aaacatgatg gatatatgaa
    33901 agacgttata aaagatagaa tagttcaatg ttttgaagaa ataaaaataa tacatgaaac
    33961 aattttagaa gaaatataaa aaaaggagaa aataaaagat gaattttaaa atgattgcaa
    34021 atataccaaa ttggacagca aaagaacaat ttgaaaaagt acttgaagaa gttttagaac
    34081 ttaaagaagc tgttcatcta aaagataata agaaaataat ggaggaaggt ttagatgtta
    34141 ttcaagcaat ttttacacta tttaaagttt tgaaaattga taaagagcta tcagagggat
    34201 tgaagttaca taacaaaaag ttaagaaaaa gaaaatggaa actggagaaa ttaaagtaac
    34261 atgggaaaag ttgaatatat tgttttttat tataattgcg aaacattcga agtgtgtaaa
    34321 aaaagtttta gtgctttaac agatgctaaa gtttttaaaa atgaaataat agaagaatat
    34381 gaaagtgttg atattataaa aagaacagtt tttgaagaat tgattttgta attaacattc
    34441 aaaaacggca aaaatataaa ttaaagtgta aaagataaat aatttggatt tacattaatt
    34501 attaaagcat tattcataaa agtatttata taaatagatt ttgtttaaac ttaaaaaaat
    34561 gaagttttat aacaaaaaaa caaaataaaa atccagaaca ttttgaataa tacataaggg
    34621 ttggaaatgc taaaaagtat ctaactagtc cccaaacctt aatttaagga ttagttaggt
    34681 atttataaca aaaagtgcaa gaggtgtaaa aatgaaaata aaaaaagagt attttgacaa
    34741 agtggaaaat gcattaaaaa tgctaggaga taaatataga aagttaagag tattaaaaaa
    34801 agaaatagca attcttaaac acaaagaaaa ctatagagaa gttaattatg aagaattagg
    34861 gtttaaagta caaaaaagtg taaaaggtat agatgacatg gttgtaacaa tagaggatgc
    34921 tatctacaac aaagaaactg aaattgaaat tatagagcga aagctagagt tttataatat
    34981 ttatttaaaa gagctatcag agatagaaca aaaaattata gaactagtat acttttatag
    35041 ctgggatggg aaaatgccta ttacaaaaat ggcaagggaa ttaaattatg atagaactag
    35101 tctttataac aaaaaagcta ttgctattaa taaaatagct ttaatgatat atggtgatga
    35161 agctttagaa taaacattat ttttacagaa aaaagactat ttttatactt cgcttctaaa
    35221 gaaaacatgt tagaattgta tcatagaaaa aagtgtacat tgacaactga atatcccccg
    35281 aaacctaaag tctaaacttg aagctcgaaa gtttaggctt ttttattgct aaaaaaagga
    35341 agggggtgag taaatgaatt ttgtagaacc tatcagagat ttagagaagc tagaaagcat
    35401 atgcgcttat ttagaagaaa ctaacatgag aaacaatgta ctcttcagta tgggcatata
    35461 tacaggactt agagtagggg acatattaaa gcttagaata aaagatgttc atgacaaacg
    35521 gtttataact cttaaagaaa gtaagacggg taagactaag cctatcgagg ttaatcctat
    35581 tttgagaaga atactaaaag aatatacaaa agataaaaat caaaatgaat ttttaattaa
    35641 gagtagagag cagaccaata aagctataag cagagtcatg gcttataaga taatacgaga
    35701 agtaggcgaa atgtttggta tagataatct aggcacacat acaatgcgta aaacttttgg
    35761 ttatcattac tataaaagaa cacatgatgt tgtaacattg cagaagcttt ttaatcatag
    35821 ttcagctacg ataaccttgc ggtatattgg aattgaacag gatgatttaa acaaagcata
    35881 taggaatatt aaatactttt agcttaagag tttaacataa aaaggtcacg ttaaactgga
    35941 taaaatatag tctaataata aagtcttgaa tgtattgtta atactaaagt gtaaggctgt
    36001 ttgataatac ttaaatgagt ttaacagaat ataccttatg ttaaactcaa ctaggaaatt
    36061 aaaggagtat cactatgaat aagtatggta agtttaaatg taataattgc ggaaagaaag
    36121 ttaatgtaaa taatagagat ttaaagacta aagttattga taatactact gttacatact
    36181 tcatgtgtcg cagatgcttt gagaagtttg ttgtatcata tgaggatgat tatattaaac
    36241 ataagagaga agaatataag aagcttaaag atgatgaagt caagctattg caagacatga
    36301 aagagtatag cgacggtcta ggagagttgt atattaatga gttataagca catgacacta
    36361 gatagattaa aagaattaat tttaattgat agacttgata tattctatag aagtacagac
    36421 tggcgcaaat taagaaaaaa agcacttgaa agagataata atgaatgtca gatttgtaaa
    36481 gaaaaaaaga aagtatctaa agccgaatgt gtgcatcata ttaaagaaat aaaagagtat
    36541 cctagcgaag ctttgaagct taataaccta acaagcttgt gcaacctttg tcacaacgaa
    36601 attcacgaaa aaatactaaa aattaatcaa gagaaaaaga aaaaattttt tagtgaagaa
    36661 cgctggtaga tagcccccct attgaaaaaa ctggctttca gctcgaaatt ttgtgaaccg
    36721 aggtaggtac atggctccgc aaggagacac caatttaaca aaaggtgtgg ggggtggggg
    36781 tcttgcaacc aaaattcatg gttgcgggtg cagaaaaaac ataaaaatga ggtggtgaaa
    36841 atggatgata atgaggaatt agaaattagg cgagacgtac agaatgacta cgttttaggt
    36901 atgacttata aagaaatgtc agccaagtat aacatacccg aaaacacttt gaaaagttgg
    36961 agaaaaagag ggggttggac tcgcaactgc accccaaaaa agggttgcgt gaaaaatggg
    37021 ttgcaagagt cgggaaataa tttaaaaaac tctataagaa aagatttatt aagacaaata
    37081 aaagagaata acatgacaga agaatacttc aaagacttag ttgaagacta tttaaaaatg
    37141 tgggatataa aaaatgaatt aatagaagat gtaaaaacta gaggtgttca agttaaatac
    37201 aaaaatggtc aaaatcaatg gggctat
//
LOCUS       DBGJFU010000025        36741 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.025, whole genome shotgun sequence.
ACCESSION   DBGJFU010000025 DBGJFU010000000
VERSION     DBGJFU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.025"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            210..845
                     /locus_tag="ACOIAS_001244"
     CDS             210..845
                     /locus_tag="ACOIAS_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGZ2700961.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            1029..1598
                     /locus_tag="ACOIAS_001245"
     CDS             1029..1598
                     /locus_tag="ACOIAS_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HGZ2700962.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            1676..1813
                     /locus_tag="ACOIAS_001246"
     CDS             1676..1813
                     /locus_tag="ACOIAS_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700963.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            1810..3585
                     /locus_tag="ACOIAS_001247"
     CDS             1810..3585
                     /locus_tag="ACOIAS_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2700964.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            4010..5317
                     /locus_tag="ACOIAS_001248"
     CDS             4010..5317
                     /locus_tag="ACOIAS_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HGZ2700965.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            5645..6184
                     /gene="hpt"
                     /locus_tag="ACOIAS_001249"
     CDS             5645..6184
                     /gene="hpt"
                     /locus_tag="ACOIAS_001249"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGZ2700966.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            6562..8238
                     /gene="bclA2"
                     /locus_tag="ACOIAS_001250"
     CDS             6562..8238
                     /gene="bclA2"
                     /locus_tag="ACOIAS_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HGZ2700967.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGVTGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGENGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVTQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(8592..9347)
                     /gene="dapB"
                     /locus_tag="ACOIAS_001251"
     CDS             complement(8592..9347)
                     /gene="dapB"
                     /locus_tag="ACOIAS_001251"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGZ2700968.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHDTLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            9787..10776
                     /locus_tag="ACOIAS_001252"
     CDS             9787..10776
                     /locus_tag="ACOIAS_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGZ2700969.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(11228..11944)
                     /gene="dapD"
                     /locus_tag="ACOIAS_001253"
     CDS             complement(11228..11944)
                     /gene="dapD"
                     /locus_tag="ACOIAS_001253"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HGZ2700970.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(12168..12917)
                     /gene="dapB"
                     /locus_tag="ACOIAS_001254"
     CDS             complement(12168..12917)
                     /gene="dapB"
                     /locus_tag="ACOIAS_001254"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGZ2700971.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(12984..13871)
                     /gene="dapA"
                     /locus_tag="ACOIAS_001255"
     CDS             complement(12984..13871)
                     /gene="dapA"
                     /locus_tag="ACOIAS_001255"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGZ2700972.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(13988..14989)
                     /locus_tag="ACOIAS_001256"
     CDS             complement(13988..14989)
                     /locus_tag="ACOIAS_001256"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGZ2700973.1"
                     /translation="MKKVNVAIIGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     regulatory      complement(15162..15336)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(15414..16295)
                     /gene="dapA"
                     /locus_tag="ACOIAS_001257"
     CDS             complement(15414..16295)
                     /gene="dapA"
                     /locus_tag="ACOIAS_001257"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGZ2700974.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGIISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            16618..17811
                     /locus_tag="ACOIAS_001258"
     CDS             16618..17811
                     /locus_tag="ACOIAS_001258"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGZ2700975.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            18017..19135
                     /locus_tag="ACOIAS_001259"
     CDS             18017..19135
                     /locus_tag="ACOIAS_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HGZ2700976.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            19266..19511
                     /locus_tag="ACOIAS_001260"
     CDS             19266..19511
                     /locus_tag="ACOIAS_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HGZ2700977.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            19498..20244
                     /locus_tag="ACOIAS_001261"
     CDS             19498..20244
                     /locus_tag="ACOIAS_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2700978.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            20529..21404
                     /gene="hslO"
                     /locus_tag="ACOIAS_001262"
     CDS             20529..21404
                     /gene="hslO"
                     /locus_tag="ACOIAS_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGZ2700979.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            21795..22595
                     /locus_tag="ACOIAS_001263"
     CDS             21795..22595
                     /locus_tag="ACOIAS_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGZ2700980.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            23122..25362
                     /locus_tag="ACOIAS_001264"
     CDS             23122..25362
                     /locus_tag="ACOIAS_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2700981.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            25776..27311
                     /locus_tag="ACOIAS_001265"
     CDS             25776..27311
                     /locus_tag="ACOIAS_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2700982.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            27327..28088
                     /locus_tag="ACOIAS_001266"
     CDS             27327..28088
                     /locus_tag="ACOIAS_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2700983.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            28426..29295
                     /locus_tag="ACOIAS_001267"
     CDS             28426..29295
                     /locus_tag="ACOIAS_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2700984.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            29624..29875
                     /locus_tag="ACOIAS_001268"
     CDS             29624..29875
                     /locus_tag="ACOIAS_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HGZ2700985.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            29987..30766
                     /locus_tag="ACOIAS_001269"
     CDS             29987..30766
                     /locus_tag="ACOIAS_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2700986.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(30901..31551)
                     /locus_tag="ACOIAS_001270"
     CDS             complement(30901..31551)
                     /locus_tag="ACOIAS_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700987.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(31548..32051)
                     /locus_tag="ACOIAS_001271"
     CDS             complement(31548..32051)
                     /locus_tag="ACOIAS_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2700988.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(32100..32615)
                     /locus_tag="ACOIAS_001272"
     CDS             complement(32100..32615)
                     /locus_tag="ACOIAS_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2700989.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            32958..33419
                     /locus_tag="ACOIAS_001273"
     CDS             32958..33419
                     /locus_tag="ACOIAS_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2700990.1"
                     /translation="MRELLKWVSIINRYNTNYIDRKLADIGINNSQYFYVLHICNNPG
                     ITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTEKSLGCYDEIL
                     KITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENGRKI"
     gene            33403..34806
                     /locus_tag="ACOIAS_001274"
     CDS             33403..34806
                     /locus_tag="ACOIAS_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2700991.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(35370..35681)
                     /locus_tag="ACOIAS_001275"
     CDS             complement(35370..35681)
                     /locus_tag="ACOIAS_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700992.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(35737..36330)
                     /locus_tag="ACOIAS_001276"
     CDS             complement(35737..36330)
                     /locus_tag="ACOIAS_001276"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2700993.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT        13900 a         4417 c         6296 g        12128 t
ORIGIN      
        1 ctgaaatagg tagtttaatt agtatatgtg gtacaaaaag ataataattt tataattata
       61 aaagtgaaat attaaatgaa atttataact aaataaaaac ttttaaacag caatcaaaga
      121 tttgtaataa aatacatccc ttataaatat atttattata taagctgata ccagaaatat
      181 aagaattaac aattgtggag ggataaaata tgatagctgt taaagatgag aataacgtag
      241 taaacttaag aggggagtta gacaataagc tagaatttag ccatgagata tttggagaaa
      301 aattttataa tatgaaaata aaaataaaca gactaagcga ttcttttgat atacttccaa
      361 tgactgtatc agaaagactt ctccaagata ttgaccttga taaacaaaat ttagtcaatg
      421 tcgtaggcca attaagatca tataacaaaa cattaaataa taaaaataga ttagtgctta
      481 cagtgtttgt cagagaaata aaaagcatag acgaggaaaa taaagaccct aatagtatat
      541 ttttagatgg atatgtatgt aaggaacctg tgtataggaa gacaccttta ggaagggaaa
      601 taaccgattt attagttgct ataaacagac cttataataa atctgattat ataccatcaa
      661 tagtttgggg tagaaatgct aaatttgcaa agaatctaaa ggttggtgat agaatacagc
      721 tttggggtag ggttcaaagt agagaatatg agaaaaaaat agatgaaaat aatgtggtca
      781 aaaaaatggc atatgaagta tccatatcga aaataaaaaa attggatgaa aataacaatg
      841 aataaacata aaaaatagac tgatttttat gaataaaatc ctaataaatt tggtataata
      901 tcatattaaa atgtataaag attgtttaaa aaaatactaa tgaaatatct cttgaatagt
      961 tgctcgccat ttattataat gtaatctgta cataatttat aattgaggaa atgaagaggt
     1021 gaagcattgt gaaaagcata ttatgtcaga taaataaaca gccggttaaa gctaaatata
     1081 catctgatga attagaatgt tttttggata caattgagta taatgtaggt gataaaatat
     1141 tgcttgttgg tcatatagga aacctaggca agagattgag aggtcttgga acttctgtaa
     1201 ctatactgga gaatagtaat tatgaagatg tctgttattc tctagtgtat aatgaaaatt
     1261 gcaatgtagt taaaggtaag ttagagtttc taccatttga tgataatcag ttcaacaaga
     1321 taataatagt tgaccaattt aatgttatta cagattgtga aaaagcttca tctgaaatac
     1381 aaagagtgtt aaagggcaat ggagaggtta tattagagga tttaaattta aagaatttaa
     1441 aagttaagtt aaactatctt aaacataaat tttgtggttc tgttgcaaag attcactatc
     1501 cacatgaagt atttaatata ttttcaagat taaactttga tggtatgatt aaagagtcaa
     1561 ataaattaaa atatatttat gtgggaaaaa gaagataaat gagttaaaat ataaatatgg
     1621 atataatata aaagactagg ttaagcctag tcttttgtta taaggagatg tttagatgga
     1681 aataaattat gagttaataa taattgttat gctctttgta atcctgatgt caatccaatt
     1741 tactttaaat aagatatact tgattcttaa agaaataaga caaatactta tgttaaagaa
     1801 aaataaggaa tgatgattat gataggttta gaatatataa aatctgatat tcaaaatata
     1861 gctgaagcta tagtatcagt actcaatata gatgtaacta tagtaaataa ggagttattt
     1921 aggattgcag gtacaggagt atatatagac aaaataggtg agaaagtaga taaatatacg
     1981 gcttttaaaa aatcacttac agagcagatt acaatactaa ttgatgaccc aaagagtagt
     2041 gatatatgta gggaatgcta taaaagtggg gcttgtgtag agtttgcaga ggtgtgttgt
     2101 cccattatat gtgatggagt ctctcatggt gttataggcc ttatagcctt tacaaaggaa
     2161 caagcacaga ttatagaaaa taataaaagt ggtcttataa actttttagg caaaatggca
     2221 gacttaatat ctaataaatt aaaagctcaa attaaaactt atgagcttga attagaaaag
     2281 aaaaaattag agacactgtt aaacaatatg gacaaagctg ttgtgtctat tgatgtggat
     2341 ggaaaaattg ataaatataa ttcaaaattt aaagagcttt tcaatataaa ggatagtata
     2401 actggcaaaa acatatttgt cgaattagat tttataaaga aaccttctat aaataacttt
     2461 aagaaagaca aatcctgtag ttttttttat aaaagatacg gatatgattc gaagggaata
     2521 tacaacatca ataaaatagt attaaaaaat gagttaaagg gatatgttat tgattttata
     2581 gataaaagag atgctataaa aaactacaat aagatgaata aagattatag aataaaattg
     2641 gaaaacataa ttggagaaag tgtagctata agaaattcta aaaaggaagc cctaatagct
     2701 tctaaatcaa catcaacagt acttattact ggggaaagtg gaacgggtaa ggagctattt
     2761 gcaagagcca tacacaacca tagcaataga gtagataatc cttttatagc tgtaaactgt
     2821 gctgctatac cagacaattt attagaaagt gaattatttg gatatgaaga gggagctttt
     2881 actggagcta aaaaaggtgg aaagcttgga atgtttgaag tagctcataa gggaagtata
     2941 tttttagatg aaataggaga tatgagtttg catttacagt caaagttatt gagagtatta
     3001 caagaaaagg aacttaataa aattggaagt aaatcaaatg tttcaataga tgtaagaatt
     3061 attgctgcta caaataaaga tttagaaaaa atggttcaaa atggcacttt tagagaagat
     3121 ttatactata gattaaatgt tatacctata aaattaccaa gtttaagaga gagaaaagaa
     3181 gatatcccac ttataatcaa gcatatggtt aaagagtatt caaaaaaatt agataaaaat
     3241 gttattgaca tagatagcag ggttgttgat gctatgatgg aatataagtg gccagggaat
     3301 ataagagagt tgcagaatat tattgaatat agtgtgaata tgtcttcttc ttcaataatt
     3361 acaatgaatg taattcctca aaaaattaag aatacctctg tagaagagtt agaagttaaa
     3421 agcgatagga taattccact tgatgaacta gagaagattg agataataaa agctttaaat
     3481 aagtacaaag attataaaaa agataaagaa ttaacagcta aagcacttgg aatatcaaga
     3541 gctacattat atagaaaaat tgaaaaatac aatataattt catagtaata taataaaaag
     3601 ctatataaaa tttaaatttt tctaaattca taactttaaa gaaagcttta aacttatact
     3661 ataagtgttt tgcagaatct ttttttatga attaaaaaat ggtttttata agttaaaaaa
     3721 taatatattt gagccgtctc aaaatgatat agaatctcat tttgagactt ttttttgtct
     3781 ttttgcgaaa ttgtcatatg tttctccatt ttaaagaata taaatactct atacaaataa
     3841 aaaaaataca taaatagagg cagtcatacc aatggttata gaatataaga ctaaaaatat
     3901 aaaaagatat aaaaaatatg gcatgtattt tgctataaat atagataaga aattatttaa
     3961 ttaattattt gagtactaaa tctaaactta attgggaggt ttatatataa tgagagatat
     4021 aagaaaagaa ttagtggaaa tgttaaaggc agaagttaaa ccagcagtag ggtgtactga
     4081 accagtagcc ttagctctag catgtgcaaa ggcaaaggaa ttattaggag aagaaatagt
     4141 tgaaaatcgt atgttagtta gtcctagtat ttataaaaat ggtatgtgcg taggtatacc
     4201 aggtactgaa agattaggat taaaaattgc agcagctcta ggtatagtag gtggacattc
     4261 tgaaaatgga ttaagtgtgt tagaaactct aacaaaagag gaagttaaaa tagctgagga
     4321 ttatatggat aatactccac tatcaataac acctgctgat acaagagaga aagtatttat
     4381 agaagttgtg ttaaaaggaa aaaatcatat tgctaaagtg agaataagaa ctaaacatga
     4441 taattttacg ttcttagaaa aagatggaga agtattatta gacaatgaac caaaagtatc
     4501 agcttcaaat gatgcggcag aaaaagctga aagtttaatg gatacagtaa ctatacaaga
     4561 attaataaaa aatgtagaag gaattgattt taaagatata gaatttttac tagatggagt
     4621 aaaaatgaat gaagagatgg ctgaatatgg attaaaacaa aaaactggta ttggtgtagg
     4681 atatggaatt aaaaagtcta tagaagaagg tcttttaggt aatgatgtta taaattatgc
     4741 aatgatgtta actgctggag cttcagatgc aagaatggca ggggtaaaaa tgcctgttat
     4801 gagttcaaat ggaagtggaa atcatggatt aacagcaatt cttccaatcg ttgcttataa
     4861 taaaaaattc ccacaatctg atgaaagact agcaaaagct ttagctatat ctcatctagt
     4921 aactggttat attaagaact atacaggaag attatctgct gtatgtggat gtggtgttgc
     4981 tgcttctact ggagcaactg ctggtatctc ttggcttatg aatggtactg aaaagcaaat
     5041 agaaggagct atagaaaata tgatagctga cttaagtgga atgatatgtg acggtgcgaa
     5101 agctggttgt gcattaaaat tatcttctgc agcatctgca gctatacaaa gtgctataat
     5161 agctaagcaa gattgttttg taccaccact aaatggtata gttggttcaa gtgttgagca
     5221 aagtatacaa aacttaggta gagtaagtga caaaggtatg tctataacag atgagataat
     5281 cttaaatgta atgaatgata tgaataaagt tgactagtac tttataatat gttttgtatt
     5341 atgaggtata acaaaaatag cataaataaa tttaattagt accctctaat aaaatatgat
     5401 tatgtataaa tttatgtata atagaaaaaa cttgttaatg caagtttttt ctttttttgt
     5461 ctaaaatata tagtatgtaa tttagtgtgg atatatgaac tacttaaaat ataagataca
     5521 ttgttaataa ttgttgaatg aacataaaaa ctcaataata ttgactaata ttgaataaga
     5581 gtattgaaaa agtgagcaat ctcttataaa attaagaagg attattacaa ttggaggtct
     5641 ttttatggat atagaaacaa aaaaatggga agtattgtat tcagaagaaa aaattaaaga
     5701 taaattaagg gaattaggag caattataga aaaagattat aaagataaaa atcttatggt
     5761 agtttctcta ttaaaaggta gctttatatt ttgtgcagac ttagttagaa atataaactt
     5821 accacttaga gttaatttta tgactacatc tagttatgga aacaatgaag aaagtactgg
     5881 aaaggttaaa gtagtttcag atgtgactac tgatatagca ggatatgatg ttttggttgt
     5941 agacgatatt actgattctg cacttactat ggattttgtg ttaaaacatc taaaagctaa
     6001 aaatcctgct agtttaaaat gctgtgttct tttggataaa ccaagcagaa gaaaagtcga
     6061 tttagtgcca gattattgtg gatttgaaat agaagataag tttgtggttg gatatgggtt
     6121 tgattttggt gattattata gaaatgttcc ttatatattc aatgttactg atgaagatag
     6181 ataagcaatt atataatttt gtggatgcct taatatgatt tttattaatc gtgttgaggt
     6241 ttttttattt aaaaagtaat taaagtagta ttaaattaat tttatgcaat ttaattatct
     6301 attttttata gcataataaa aaaattttaa ataattgatg ggaaatgagt tttttaattg
     6361 tattaataat tgtaaaagag tactaataat tgtaaaagat tttacaaaaa agtaatgaaa
     6421 tttaagaatc aaagcatata tgttgatata agcaaataga tacattatca cataagaaat
     6481 tgtcgattca tataattata tagagtaata ctcaatattt atagagtatt actctaactt
     6541 taaaaaagga ggattaatta tatgagtgat atttcaggtc caagtttata tcaagatgta
     6601 ggtccaacag ggccaacagg tgctactggt ccaacaggac cgacggggcc tagaggtgta
     6661 actggagcga ccggagcaaa tggaataaca ggaccaacag gaaatacagg agcaactgga
     6721 gcgaatggaa taacgggtcc aacaggaaat atgggagcga ctggaccaaa tggaacaaca
     6781 ggttctacag gaccaacagg aaatacagga gcgactggag cgaatggaat aacaggtccg
     6841 acagggaata caggagcaac cggagcaaat ggaataacag gaccaacagg aaacaaagga
     6901 gcaaccggag caaatggaat aacaggttct acaggaccaa caggaaatac aggagcgact
     6961 ggagcaaatg gaataacagg tccaacaggg aatacaggag caacaggagc aacaggacca
     7021 accggactaa ctggagcaac aggagcaacc ggagcaaatg gaataacagg accaacagga
     7081 aatacaggag caaccggagc aaatggagta acaggtgcta caggcccaac aggaaataca
     7141 ggagcaacag gtccaacagg aagtatagga gcgactggag caacaggaac aactggggca
     7201 acaggtccaa taggagcaac aggagcaaca ggagcagatg gagaggtagg tccaacagga
     7261 gcagtaggag caacaggtcc agatggtttg gtaggtccaa caggcccaac aggcccaacc
     7321 ggagcaaccg gagcaaatgg tttggtaggc ccaacaggcc caaccggagc aaccggagca
     7381 aatggtttgg taggtccaac aggagcgaca ggagcaacag gagtagctgg ggcaataggt
     7441 ccaacgggag cagtaggagc aacaggccca acgggagcag atggagcagt aggtccaaca
     7501 ggagcgacag gagcaacagg ggcaaatgga gcaacaggcc caacgggagc agtaggagca
     7561 acgggagcga atggagtagc aggtccaata ggtccaacag gtccaacggg agaaaatgga
     7621 gtagcaggag caacaggagc gacaggagca acaggggcaa atggagcaac aggcccaaca
     7681 ggagcagtag gagcaacggg agcaaatgga gtagcaggag cgataggacc aacaggccca
     7741 accggagcaa atggagcgac aggagcaaca ggggcgacag gagcaaccgg agcaaatgga
     7801 gcaacaggtc caaccggagc gacaggagca acaggagtgt tagcagcaaa caatgcacaa
     7861 tttacagtgt cctcttcaag tttagtgaat aatacattag tgacatttaa ttcatcattt
     7921 ataaatggaa ctaatataac ttttccaaca agtagtacta taaatcttgc agttggaggg
     7981 atatacaatg tatctttcgg tatacgtgcc acactttcac ttgcaggatt tatgtcaatt
     8041 actactaact ttaatggagt aactcaaaat aactttattg caaaagcagt aaatacactt
     8101 acttcatcag atgtaagtgt aagtttaagc tttttagttg atgctagagc agcagctgtt
     8161 actttaagtt ttacatttgg ttcaggcacg acaggtactt ctgcagctgg atatgtatca
     8221 gtttatagaa tacaatagtt tatatgatga aaagattata aaataataaa caatatgatt
     8281 tcatatacca ataaatagaa taatatttaa taggtttata tgatgaaaag actataaaat
     8341 aataaacaat atgatttcat ataccaataa ataggataat atttaatagg tttatatgat
     8401 gaaaagacta taaaataata aacaatatga tttcatatac caataaataa gataatattt
     8461 aagaaaaatg attatattta aaaggtaagc ataaaattta taaactttta gaatagaaat
     8521 ctcaatcaat tagatattca aattgatgaa tatgaatacc taaatagatt gagatttatt
     8581 atgtacaaaa cttatcctaa tatatctgac attttataaa gcccagattg cttaccaatt
     8641 aaaaatttag ctgcaactat agagccgttt gcaaatactt catcagattg agaatgatgg
     8701 cttaatgtaa gaacttcatt atcacctgca aatacaacat catggtcgct gattatattg
     8761 ccacctctta tagagtgtat tccaatttca tttggttttc ttttacaatc tctaccatat
     8821 ctaccatgtg tattttcact ttcaggcaag acttctctaa ttgcattagc tatcatagta
     8881 gcagttccac taggggcgtc ttcttttcta ttgtgatgtt tttctatgat ttcaatatca
     8941 aaaccttcta acatctttga agcatcctta actaatttta ttaatatatt tactccaaga
     9001 gatgtatttg atgataataa tataggaatg ttttttgatg cttcttctat tttatttaag
     9061 tcattttcgt catatccagt agtagctaat actacaggtg tattattttt tagagcatat
     9121 gacaatattg gctctaaagt atcatgatgt gaaaagtcta taattacatc agttttttca
     9181 gttacatcag tcattgtttt gtaaagcttt gtatcactat gatgaccttt ttcatcagca
     9241 gacctagcta caccacaaac caactctaat tcattatctt tttttacaca ttctgctaaa
     9301 actcttccca ttgttccatt atagccacta attataactt tcaacataaa ttcctcctat
     9361 gaaattaata aaaatttgtt atatttctat aagtttattt tatcactaat ataatttata
     9421 taaaataata ttagtgataa taagcttaca atttatctta cattaaaaaa tttaagtgga
     9481 tttttcaaaa tatagataaa tttctttttc attgtttaaa tgataaaaat atatttgact
     9541 tcataccaaa aagagtgccc catgaactaa aaagttaatt ttgaaatacc atattcatat
     9601 atcatatgat ttatttggaa attaatatta atacattaaa ataactaaat acatgtatgc
     9661 ataaagatgt ataaaaaata tataaacata tagtattaat tttaaattca acaatttaat
     9721 tgttaaaagt gttattatta aattagaaat atcttaactt aatatactga gatgggagtg
     9781 atagatatgt taaaagaaga aaaaacttat caagagaaag acgcaaaagt attcaatgga
     9841 gtggcagctt tgataatgat tttattattt ttagtagttt caataggtat catggttttt
     9901 gggatagtaa aacttgatag tggatttact ggaataggtt cacttatgtt aattttggga
     9961 cttgtattta tagtagtaga ttttatccta ttttttgggc taaaaatgat taatcctaaa
    10021 gaagctatag ttctagtttt gtttggtaac tattatggaa ctataaaaaa agaagggtac
    10081 tattgggtga atccattttg ttcagcaata aacccagcag tatcaagaat taatgtatcg
    10141 aaatcttcat ctgatgaaaa ggtagatata tcaactaatt caaggggcaa aaaagtatct
    10201 ttaaaaacaa tgactcttaa taatgaaaag cagaaagtca atgatttatt gggaaatcca
    10261 attataatag gagtggttgt aatttggaag gttatagatg caacaaaggc agtatttaat
    10321 gtggataatt ataatacatt cttatctatt cagtgtgatt ctactattcg caatgtttca
    10381 agactttatc catatgatgt atctgaagat ggagatgaaa aatctcttcg tggtagtagt
    10441 caggaaatag cagatagatt aaaagatgaa ttacaatcaa gggttgacat tgcaggaatt
    10501 gaagtgtgtg aagtgagaat tactcatctt tcttatgcac cagagattgc agcagcaatg
    10561 ttacaacgtc aacaagcaga ggctataata gcagcacgta aaaagattgt tgagggggct
    10621 gtaagtatgg tagagatggc attgactaat ttaagtgaaa ataatgttgt gactttggac
    10681 gaagaacgta aagcagctat ggtaagtaat ctgttagttg tgctttgtgg caataaagat
    10741 gcacaaccag tggtaaacag cagttctatt tattaaatta taaaaagttt aatagtttat
    10801 tttattgata tatagaaaat ttagtagttc attttattaa attatataaa gattaatggt
    10861 ttgctttatt gaattataaa aagtttaata atttattcta ttgatatata gaaaatttag
    10921 tagttcattt tattaaatta tataaagatt aatggttttc actttattaa attataaaaa
    10981 gtttaatagt ttattacatt gatatattga aaaattgaag gaaataaaaa tacttgtcag
    11041 caagaaataa aaatgatata agaatgaata agagaattgt aaaaaaataa aatgattata
    11101 aataaaagat aaaaatattg taaaaaagag ggtgcctctg gtgaattaaa ttcacacaga
    11161 gacacctttt taattaattt tttaaattag tatattattg tttaattaat agcatagtac
    11221 tatttaatta atctaaattt ctaaggtctt ccataagctt agttttgtca gccgtttttt
    11281 catctttcat ttttataact ttagcaggag aaccagcaac aacggcacca gcttctacat
    11341 cagtagttac aacagcacca gctgcaacaa cagcaccttt accgattcta acaccttcaa
    11401 gaataactgc attagctcct attaaaacat catcttcaac tataacagga gtagcagaag
    11461 gtggctctaa aacaccagca acaacagcac cagcacctaa gtgaacattt ttaccaagag
    11521 ttcctctagc tccaataaca gcattcatat ctaccataga accttcacca ataacagctc
    11581 ctatatttat aacagctccc atcattacaa cagcattttt ttctatagta accatgtctc
    11641 ttattattgc acctggctca attcttgcat gttcacttaa agtatttttt agaggaatag
    11701 cagaatttct tctatcatat tctacatgag tatctgttat atcatttcca tgtttatcta
    11761 atacttccat tatatcctca tagtcaccta caaatatata agaaccatta cttccaaata
    11821 ccttaaattt atcagttgat tggaagtgta aagagttagt atttacatat accttaacag
    11881 gtgttttctt tttaacttct tttatatacc ttgctatttc ataagcatcg tttaaattta
    11941 ccatttgtta attctccttg tatttcatta tatttctttt attatatact tatttgtcaa
    12001 ttttagtata tacttacttc ttattattta tattttattt tagttagctt ttattgtata
    12061 gacatagtat taaataaatt tattatatat gtttgatttg tattgcatat tattatatat
    12121 atattgtata tgtttaattt atattgtgta ttattatatt tatactatta tcccaacatg
    12181 ctgtccatat tataaaatcc tggtttttgc ttaactaagt atttggcagc agctatagaa
    12241 ccacttgcaa agataccacg agattgagct tgatggctta ttgtaagaac ctcattgtct
    12301 ccagcaaata gagcatcatg ttcgccaaca atatttccac ctcttatagc atgaatacca
    12361 acttcatttg gttgtctttt tgcactacga ccatatctac cataattatt ttcaacgttt
    12421 ggcaaaacct cttttatagc gttagcaatc ataacagctg tgccacttgg ggcatctact
    12481 tttctgttgt gatgtttttc tattatttct atatcaaatc cttctagtaa tttagctgca
    12541 tctttaacca attttactaa tacatttaca ccaagagaca tattatatga ttggaataca
    12601 ggaataactt tagccgcttc acgtattttg ttcatttcgt catcattata tccagtagta
    12661 gcaattacta aaggtgtttt agtctttaaa acgtaagata agatattatc taaattagag
    12721 tgatgagaaa aatctataac tacatcagct tcctcaacta tagtactcat atcttcataa
    12781 attttaaggt cgccttcata gctatccaga cctctagctg caacacaaac caattctaat
    12841 tcattatctt ctcttatagt atcggctaaa atcttaccca tttttccatc ataaccagtt
    12901 gctataactt ttaacataat ttcctcctat gattaagggc taacttttga ccaaacttac
    12961 tttttaccat gcattaatct ttactatgca ttaactttta gtccaaagtt aactaattct
    13021 ttctttaata cttctaaatt tgctgggtcc atttctgcta gaggtaatct taaatctcca
    13081 acattgaatc ccaataaatt cattgcagtc ttaactggta taggatttac ttctataaat
    13141 aaagctgcga ttagagcatc cataccaagt tgtaattttc tagaaccttc aatatcgcct
    13201 tcaaagaatt tagctaccaa atcgtgagtt tcttgagggc aaacattagc aagtactgat
    13261 ataactcctt gacctcctaa agataataaa ggaagtatag tatcatcatt accagagtag
    13321 atagcaaagt catctggaac taaactagct atttctgcaa cctgtcctag gtctccacta
    13381 gcttctttta cagcaactat gttatcaatt ttagcaagtt caactataag gctaggactt
    13441 atatttacac aagttcttcc agggacattg taaagtataa ttggaagttt aacactgtta
    13501 gctattgttt caaaatgacg cttaagtcca gctttattag ctttattgta ataaggtgtt
    13561 attattaata gagcatctac tcctaatttt tcagcttctt gacttaagtt aactgagtgc
    13621 atagtatcat tagaaccact tccagctata acaggaactc ttttattaac tttttcaaca
    13681 acatacttaa tagtagcaag ttgttcttca tcagtcatag tattagcttc accagtagta
    13741 ccacaagata ctatagcatc agtaccatta gctatttggt attcaactaa ttcacctaat
    13801 ttgtcaaaat caactttgtt atcctttgta aatggtgtaa ctagagcaac agcagaacct
    13861 ttaaatatca tataaaatca atcccctctt ttttattttt gtgttttata ttacagttac
    13921 ttctaatgct tgtttttgag ttactattaa tattttacaa aattatatat ttatacaagt
    13981 ttgtatatta tactaaatcg tatgataaag ccatttcagc tatttgaatt gcgttagttg
    14041 cagcaccttt tcttatatta tcagcaacaa cccaaagatt tacaccatta tcaacactaa
    14101 aatctcttct tattcttcca acaaatactt catctctatc atcaaattct acagcagttg
    14161 gatattcgtt tttagcagga ttatcaacta ctacaacacc tggagcttct tcaagtgttt
    14221 ttactaaatc atctaactca aatggattgt taaactcaac atttatagat tcactatgac
    14281 cattttttac tggaactctt acagtagttg cagttatttt taaatcgtag ttatcaagta
    14341 ttttcatagt ttcatttacc attttcattt cttctttagt gtatccattt tcagtgaatg
    14401 aatctatatg aggaagacag ttgtaagaga tttgatgagg gtagaatcca gtaggattac
    14461 cgttcattcc tttctctaaa tcttcaacac ctttaacacc agaaccagat acagcttggt
    14521 aagtagagta aacaactctc ttaattttgt atttatcatc aagagcttta aggggaacca
    14581 tagcttgtat agtagagcag ttaggattag ctattatacc cttatgattt tttatggctt
    14641 ctggattaac ctcaggaact actaaaggca catctggatt cattctccat tgactagagt
    14701 tatctactac taagattcca tttgaagcag ctattggagc atacttttca ctaatacttc
    14761 caccagcaga gaataatgct atttgtatgc ctctatcagt aaatgaagtt tcctttaatt
    14821 cttctactat atattcttta ccacaaaaca ttactttaga accagctgat ttagaagaag
    14881 agaataaaaa tagattttct attggaaaat ttctttcttc taatactttt aaaaatgttc
    14941 tacctaccat tccagtagca ccaattattg ctacatttac ttttttcata taaattcacc
    15001 ccaattttaa attattaaaa cattatttta ttttagttat acaagtaaga tatgttaata
    15061 tttttatata tccaaaaact ttaatattat attaacaact ttaattatat acaaaaaacg
    15121 tacaaaggta tactttgtac gtctaattta tcactcaaag taaaccctgt agctcatcac
    15181 ctacaaaaat aagtgacagt attacacatg ttatgtgcaa tcccagaaaa tattattgca
    15241 agaaatattt tcttcggctg taaatccttt ttccaatcat taacacctgc cagctactat
    15301 tggatactct tagttacagc tacctctacc ctaggttaat aggatattaa aatttgatta
    15361 ttctaataat aatacatatt taagaaaaaa tcaacttatt tatataaata ttgctaaaat
    15421 ttaaatccaa aatttgtcaa ttcttgtttt aacacattaa gattagtttc ttccatttca
    15481 gctagaggaa gtcttaaatc accaacatta aaacctaaaa tgttcatggc agttttaaca
    15541 ggaactggat ttacttctat aaataaagca gcaattaaag catccatatc aagttgtaat
    15601 ttttttgagc cttcaatatc accttcaaag aatttagtta ctaaatcatg tgtttcttta
    15661 ggacatatgt tagcaagtac agagataata ccacttccac ctaatgataa tagtggtagt
    15721 atagtatcat cattaccgga gtatatagca aagtctttag gtactaattt agctatttct
    15781 gcaacttgag ctaagtctcc acttgcttcc ttcacagcaa ctatattatc tattttagca
    15841 agttcagcca caacagatgg ctttatattc actttagttc ttcctggtac gttgtataaa
    15901 ataattggca acttaacgct atttgctatt gtttcaaagt gaagtttaag acctttctca
    15961 ttagtcttat tatagtaagg agttattatt aaaagaccat ccacccctaa tttttcagct
    16021 tcttgactca tatgaacaga caacatagta ttgtttgaac cagtacctgc tataacaggt
    16081 attctcttat taactttttc aactgtatat tttattacag caaaaatttc agattcactc
    16141 atagtagtcg cctcacctgt tgttccacaa acaactagtg catctgtgcc attctcaatg
    16201 tgatattcga ttaattcacc taatttttca aagttgacat tattatcctc tgtaaatggg
    16261 gtaactaaag caacagcaga acctttaaat aacatataat caaatctcct tttttatcta
    16321 aatttaattt tttaagtaca ataataatta tagtacaaat taattaattt taataggtat
    16381 agttaatatc ttaactgttt aaataagata tttatattat attaatatta gaaaaatttt
    16441 aaataaatgt tttattctca aaatatgcat attaatttaa taaaatatac caaaatggaa
    16501 ataaaaaata aattttatga gaatttaata actaataatt aatatattta ggttataata
    16561 aatatgtgcg taaaatctaa caataagcaa taataccgta aatttaggag gaataatatg
    16621 gatactttaa aggaactttt taaaatagga agtggacctt caagttctca tactatggga
    16681 ccacaaagag cagcagagag atttaagaat gaaaacccag atgcagaaag ttttagagca
    16741 atattatatg gtagtttggc agctacaggg aaaggtcact taactgacta tataattgaa
    16801 aaaactatag cacctaaaaa agtcgaaatt gtttgggaag aagatattat aaaagatttt
    16861 cacccaaatg ggatgaagtt tgaagcttta gataaagata aaaatgtaac agctgaatgg
    16921 actgtatatt ctgtaggtgg aggaactata gcagaagaag gtcaaagaaa cagtaaaagc
    16981 aatagtatat atcacttaga tactatggat gagatagtaa aatggtgcaa agaaaataat
    17041 aagactttgg tagattttgt gttggaatgt gaaccaaaag acataaaaga ttatataaaa
    17101 actataaaag atgctatgag aaaatctatt gatgatggac taagtacaga tgaaataata
    17161 ccaggcaagt tattattaaa aagaagagca agtacatttt ataatgcata taaaaaagat
    17221 aagagcttct caacacttgt atatgcatat gcactagcag cttcagagca aaacgcttct
    17281 ggtaatataa tagttacagc accaacttgt ggttctgctg gagtaatacc aggaatattc
    17341 tttgcaatgc aggatttcta taattatgat gacgaaaaaa taattgaagc actacttgta
    17401 gctggaataa taggtaatat aataaaaact aacgcttcaa tatctggagc agaggtaggg
    17461 tgtcaaggtg aagttggagc cgcttgttct atggcagcag cagcagtagc atacttaaaa
    17521 ggtggaacta tagaccatat tgaatatgcg gcagaaatag cacttgaaca tcatttagga
    17581 atgacttgtg accctgtata tggatatgtt caaattccat gtatagaaag aaatgcaatg
    17641 gcagctcaaa gagcttatga tgcagctaat tatgcattat taactgatgg ctcacattct
    17701 gtctccttag accaagttgt tgaaacaatg aaagaaactg gtatagatat gatggataag
    17761 tacaaagaaa cagctaaggg tggacttgct aaacatttct tctcttgcta aaaataaaat
    17821 tttccaataa taattcctct gttttgattt atgaaacagg ggaattatta ttaatggtga
    17881 attatttaat aatgcaaaat atttttatat tcaatttagc tttttatttt taattagtat
    17941 aacatgctat ataatatttt aattataaag aaatatagaa atatttttta ataaattaga
    18001 aattggagga gattacatga gagaggcatc atatttacaa gaacaacttt ataaacatag
    18061 gaagtcatta aatcctattg cagagattgg gagaaaagag tataagactt caaaatatat
    18121 aagagattat cttgataaat taggaattga gtatgaagtt tatttagaaa caggtatagt
    18181 aggtgtaata gaaggtaaaa gacctaaaaa agatattgct tttagagctg acatagatgg
    18241 tcttcatagt gaagaagggg taaaacattt atgtggtcat gatggacatg ctactatctt
    18301 acttggatta attgagtatt taaatgataa taaagaagac ttaaatgata atatagtatt
    18361 tatattccaa ccggcagaag aaggtcctgg gggagcaaaa gctttaatag atgaaggagt
    18421 gcttagaaaa tacaatattg atgaaatata tgggcttcat atatatccag agttaccaga
    18481 aggttatgta ggtacaagag agggctattt tatggctcaa ataggagata ttgacataga
    18541 aatagtgtct aaaagtggac atggggctat gccacaaaat ggtattgatg gtatagtgat
    18601 agcttcaaac tttgtaagct cacttcaaac tatagtgtct agaaatataa gcccaataga
    18661 caatgcagtg ttgactattg gcagaattga agggggagct agaagaaata taatagctga
    18721 aaatataaag atggaaggta ctatgagatg tttcagccca gaagtgtatg agaaaatgaa
    18781 gcttagagtt agagaacttg ctagagggtt tgaattggct tataactgta aggttaatat
    18841 aaatattata gatgattata tcgctgttaa aaatgacaag gatttgtatc aagagtttgt
    18901 agaggctatt ggaagtgata ctgttgtaga gttagagcca cttatgatat cagaagattt
    18961 ttcttattat caaaaagaag ttccaggatt attttttatg ttgggttcaa gaaacgaaga
    19021 aaaaggattt gtaaatggtc ttcataatat aaactttaat tttgatgaaa agatatgtgt
    19081 aaatgcattg aatgtttatt caaaattatt aaaatacaaa gaagcaatag attaggaaga
    19141 taagttataa agattaaaaa gcataaatac ctaaatttaa aaaaagtgta ttaataaata
    19201 ccccttacat atttgtataa atttatttta attgacaaaa atacaaataa gaaggggtga
    19261 tttttttgaa agaaaaagta caaagagaaa agaagataac taatggcaag aatactaaaa
    19321 tattgacaaa tgatgatata atgaaatatg agatagcctc agaactaggt cttatggata
    19381 aagttggtga acgtggatgg gcaggactta cagctaagga agcaggaaga attggaggta
    19441 tgcttacatc acgcaaaaag aggcttaaaa agattgtgga ggagcagaat aaaaaggatg
    19501 agtcaatata gtgattttgc gtttgtatat gatgaactta tgaatgaagt tgattataat
    19561 ggttgggtta aatatataga agatataata gatagtgaag gtgtaaaagt aaaaaatata
    19621 ttggaattgg catgtggcac gggtaatctt acaattccat tgactaaaaa aaattacgat
    19681 atagctggta tagatatatc tgatgagatg cttagtgtag caagagaaaa gtcagaaaaa
    19741 gaaggagtag aattagtttt acttcaacaa gatatatcag agcttgattt tgaaatatct
    19801 gatttggact gtgtactttg tgcttgtgat ggattcaatt atataactta tgatgatgat
    19861 ttggaaaatg tattttcaaa gacatatgaa ttattaaaga aagatggaat attcatattt
    19921 gatataagtt ctttttataa attagccaac atattgggaa ataacatgta tggagaaaat
    19981 agagaagata tagcgtatat gtggcaaaat tactttgatg aagaagagaa tctaattgaa
    20041 atggaactta cattctttat taaagatgaa tgtggaaaat ttgaaagatt tgaagagata
    20101 caccaacaaa gagcatatac tgaagatgag atagttgata tgcttgaaaa atctggattt
    20161 aaggatataa aatattatgg ggattttaca tttgacaaac ctagtgaaga aagtcaaaga
    20221 atcttctttg tatgtaaaaa ataagatatt tttgactata taagataatt attaaaaata
    20281 accatgatat aaaaatatag aaaatgcagt tttatagatt aaaagtaaag tgatgatact
    20341 ttatgacttt tattttttaa taattataaa gttctataga taaaaattaa aatataataa
    20401 ctattgctta gtaaaattaa aataaaatgg gttttgccca aggttaagat agataagtat
    20461 ttatagatta aaattaaggt agtaatataa taaaaataag acaattaacg ggacatggag
    20521 gaatattaat gagagattat gtactaagag cgacatcagg aaatggtcaa gttagagcat
    20581 ttgtcgcaac tactagaaat acagtagaag aagctagaag attacatgaa actacaaaag
    20641 tagcaacagc agcacttgga agaacattaa cagccacatc tataatggga ttaatgatga
    20701 aaaatgatag tgataaattg actgtaataa taaaaggtgg aggtccaatt ggaactatta
    20761 tcgccacatc agactctaag ggaatggtaa aaggatatgt aggaaatcca caagtagagg
    20821 ttgaagacta tccaaatgga aagttaaatg tagcagcagc agtaggtaca gaaggtgttg
    20881 taaaagttat aaaagactta ggtcttagag aaccatataa tgggacttat ccattggtaa
    20941 gtggagaaat tgcagaagac tttacttatt attttgctgt atcagagcag actccatcag
    21001 tagtagcttt aggtgttctt acaaaagaag atgaagtaga gtttgctgga ggatttatag
    21061 ttcaattaat gccagatgca gaagaagaga ctattgctaa gttagaggaa aatgtagcta
    21121 aattaccttc tataactaac atgttaaaag aaggaaaatc accagaggat atattaaata
    21181 ttgttttgga tggtttggaa cctaaaatac ttgatacctg tgaagttggg tttatgtgtg
    21241 aatgttcaaa agaaagagta aaaactgctt tggttgcaat tggtaaaaaa tcattggctc
    21301 aaataataga agaagataaa aaagcagaag ttggttgcca gttctgtaat aagaaatata
    21361 tgtatagtga agaagagttg cttgaaattt taaaggaaat gtagactata aaaatagttg
    21421 tattgatata agtaatgtta tagtgatata gtaaagtcgt attgatatag taattaaagt
    21481 ataatttatt aataatagac atatttatat taaaaaataa gtttaataaa tttttaagct
    21541 taataaattt taagaattaa gaagctttct ggctgtaatg tttgcaatgt tgcagttaga
    21601 aggctttttt attttattat agttcgtgtc aaagattgta tgaaacaata aaatattgta
    21661 aaataatttg tatatttaga cctgattggc acaaaggttt ataaggtatt tgagttgatt
    21721 atgcgaaatt ctatgaagta gtaaccctgt gactttaatc atgggaggtt cagaagaagg
    21781 agaaaatttt aaatatggaa agtaaaacga agtacatagg aataagtata ttaatattat
    21841 tgatgatatt tttaattgca aaaaatttta acggaaaact tgaaatacct attcctgaga
    21901 gtaatagtag aaaatttaaa tcagcagcag tgttttatcc tcaacatcaa gatgatgagg
    21961 ttttatgggg aggaagtgcc attgtagatg ctataaaaca gtgtggtgtt gataatgtat
    22021 atgtggtatt agtatcagat ggttctggag ttaacgtttt caaagctaac acaaaattta
    22081 gaaatttaac taggaagcaa aaggaagaac ttagaaataa cgagtttaaa tcagctttga
    22141 gagagcttgg agtgaaacct caaaatgtga ttatacttgc agatatagat aaaaaggaag
    22201 gcacacatta tgaattaatg gaaaagacta tattggattt tgaacataag tttaaaggca
    22261 atgtgaccca tatagcccat cactataagt atgatgacca tataatgcat agaaagaatg
    22321 gggaagtttt aaagaaactt aaacgtgaag gtaagataaa agatgacttg tattttgtta
    22381 agccaaaata tgtaaagtat ataccaagaa atgaaagagc tatctataaa gcaatggatt
    22441 ttgatgacta caataaggtt aagagtgctt gttatgaata taaggttgta aattcaaagc
    22501 gtaaaatgta tggtatagga tatatttcgg cacatagtta ttttgaacat cttctaaatg
    22561 accctgaact tacgtctgta ttaagtgaag attaatttct tataagtagt aagttatgtt
    22621 tgataagttg attggaattt tctatagttt tgaaggtatt ggaaatttag ttttatttaa
    22681 agatttaaag atagaggttt ttagtgttta aaattttaaa gttaagttga ataattgtat
    22741 agaattattt gtgaaagaag aaacagtgta aaacatagta tcttatttat ggttttaaaa
    22801 tcataggcaa gatacttttt ttatgtaact aataccattt tgagattatt aacttaagaa
    22861 ttttatataa tttaacactc atatataatg atgatattta ggtagaatag atgcaattac
    22921 gcttaaataa aataactgta ttttttataa aaatcaaacc ctacactata ctaccagata
    22981 aaatctacat gctataattt gacctgtgtt tataaaaaaa ggagtaatct aataaaatag
    23041 tatttcactt atgtataact aacaactatt attacgattt tctctaaata atttaaagaa
    23101 aaataatgca ggaggtaaaa tatgaacaat gaatttataa ttttaaaagg ttgcaaagag
    23161 aacaatttga acaatatttc cttaaaaata ccaaaaagaa aaataaccat attcacaggg
    23221 gtgtcgggtt ctggaaagtc atctattgta tttgagacaa ttgcaaaaga atcacaacgc
    23281 caactaaatg aaaggtttag tacatttgta agaagttttt tacctaagta tggagaggtt
    23341 aaagctgatt gtatagaaaa tttgtcaaca ccaattatca tagaccaaag tagacttggt
    23401 ggaaattcaa ggtcaaccct aggtactatt acagatgtaa attccttttt aagagcttta
    23461 tattcaaggt ttggtagtga gtatataggt aatgctaata tgttttcttt taataatatt
    23521 gatggtatgt gcccacactg tcatgggctt ggaaaaaagc tagttcctaa tatgaaccaa
    23581 attcttgata tgaataaatc attaaatgaa ggtgctatat tgctttcagg gtttggagtg
    23641 ggttcttggc attggaatat attcgctgaa tcaggatttt ttgatattaa taagaggata
    23701 tgtgattatt cagaagtaga attggaaaag tttttgcatg gtaaagcaga aaagattaag
    23761 atagaaaatg ctggtcaaac taacatgaca tatgagggat taatggttaa atttaatcgc
    23821 ctatacttaa gtagagaggg agaaatatct gaagctacta agaaaaaatt gagtaaactt
    23881 cttatagaag atagatgtcc tatatgtgat ggaagaagac ttaatgaaag agtatatagg
    23941 tctttaatta atggttataa tatagcagat ttaactagta tgcaaattga tgaattggct
    24001 gaagttatta aaagtataga tgagccagaa gcacaacctt taattaaagg aataatagaa
    24061 aagttaaaca gtatcataga tataggtctt ggatatttaa cactggatag agagacatct
    24121 tctctatcag gaggggaatc tcagcgtata aagatggtta agtatttaaa tagtaatctt
    24181 gttgatttaa tgtatatttt tgatgaacca agtgtaggac ttcatccaaa agatgtgtat
    24241 aaattaaata atcttttaaa aaaattgcgt gataaaggga atacaattat agttgttgaa
    24301 catgacccag atgttattaa gattgcagac catattgtag atgtaggacc aaaggcaggt
    24361 aagtatggtg gagaagttgt gtatgaaggc agttatgaaa atctacttac aagtggtact
    24421 ttgacaggga atgctttaag taaatttctt cctattaaag aaagtgtaag agaacatagt
    24481 ggatatttag aagttaaaaa ttgtaataaa aacaatttaa aaaatgtatc gattaagata
    24541 cctaaaggtg tattgactct agttactgga gtagcaggag caggtaaaag tacgttgatt
    24601 aaggatgaat ttttaaagca aaatcctagt gctgtactta tagaccaaag ccctgtttca
    24661 gcaaattcaa ggtcgagttt ggcaacatat agtggaatta tgaacaacat aagaagcata
    24721 ttttcaaaaa caaatggcgt aaatgcttct ttattcagtt caaattcaga aggagcttgt
    24781 gataactgta agggttctgg aatagtagaa atgaatcttg cttttatgga gagtataaag
    24841 agtacctgca atgtttgtga aggtaaaaaa tttaaaaaag aagttttaga atataggttt
    24901 caagggaaga atataattga agttcttgaa atgtctgttt tagaggcaat tgagtttttt
    24961 aatttaaaac agattaaaac gaaattacaa tgtattgaaa aaatgggtat tggatacctt
    25021 actttaggac aaactcttga tactttgtct ggaggagagt gtcaaagatt aaagcttgct
    25081 agtgaattaa ataaggaaag ttcagtgtat atattagatg aaccaacaac aggtcttcat
    25141 atggctgata ttgagaattt tataagtata attgaagaga ttgtagataa tggaaatacg
    25201 gtcataataa ttgaacacaa tatagatgta attaaaagag ctgattggat tattgagctg
    25261 ggacctgaag gaggaacaaa aggcgggaga gtaatatttg aaggtattcc aaaacaatta
    25321 tgtaacaata aaatatcatt aacagcaaaa tatatagtat aataaataaa atataataaa
    25381 attataatga tattttaata aatataatgg gtatataaaa tgataattaa acttaacagt
    25441 aataaataat gtgagaaata ttcaaattta attaaataaa gtgtcagaaa tttgtttaaa
    25501 ttttagtatt ttaacggttc tgaccttttt tcttattttt atggagaata tcgaggatgg
    25561 agtctctaat agttactttt ttgtgtctac ttgcaagttt gattaaaaaa tggctataca
    25621 cacttgtgac ttcagtcatg ggaggttcag attataatct taatatataa tttgtgaaat
    25681 aatttgatga cgatttgctg atgtataaac aataaagatt aactacatta aaatttaaat
    25741 cttgttaagt aaaacttata aaaggagaga gatggatgat aaatgatatt ctaaccttat
    25801 tatctgaaag aagtgatttt tttataaagc tatccataga acatttgatg atttctatta
    25861 tttctataat aattgcgatt gttttgggtg gtataattgg tattattgta agtgaatttc
    25921 ataattcttc taaacctata ttgggaacta tcaattttct atacaccatt ccatctattt
    25981 ctatgctagg atttctgata ccattttccg gaattggaaa tactactgct gttattgcac
    26041 ttaccattta tgcattattg ccaatggtta gaaatacata tactggaatt actaatgtgg
    26101 ataaaaatat tcttgaagca gctaggggaa tgggaagtac agattttcag atattgtata
    26161 aaataaaact tccgttagcc ctacctgtta ttatgtctgg tattagaaat atggtgatta
    26221 tgacaattgc attagctggt atcgcttcat ttataggtgc tggaggtcta ggtgtagcca
    26281 tatatagagg aatcacgaca aataacacaa taatgacaat tactggaagt atacttattg
    26341 ctctattagc tttaatattt gattttattt tagggctggt agaaaaattt tttcaacgaa
    26401 aaagaaaaaa gaaaaatagg aaaaaagttt ttgccattct aggaagcctt attcttatag
    26461 caactatctt tacgtcttat ttatttaatc cttcaaagac tcaaactatt catattgcta
    26521 ctaaacctat gactgaacaa tatattcttg gtgagatgtt ggatatttta attgaaaaaa
    26581 acacaggtct aaacgtggaa attacacaag gtgtaggagg gggaacatct aatatccagc
    26641 cagctatgga gaatggagaa tttgacattt atccagagta tacaggtaca ggctggaata
    26701 tggtacttaa gaaaaatgaa ttatatactg aaagtatgtt tagtaaacta cagtcggaat
    26761 acaaaactaa acttaaaatg caatggactg gtatgtatgg tttttctaat agctatgcat
    26821 tagcagttag aaaagaagtt gcagataaat acaatttaaa aaatataacg gacttaaaag
    26881 atgtatcttc taaattgaca tttggagctg aatatgactt ttatgagaga gaggatggat
    26941 atactccatt gtgtaataca tatggactga actttaaaga tacagttgat ttagatattg
    27001 gattaaaata ccaagctatc aatgaaggta agattgacgt aatgcctata tttacaactg
    27061 atggtcagta tagtgtttca gatgttgtag ttctaaaaga tgataaaaat ttctacccat
    27121 cttatcaatg tggaaatgtt gttcgtttgg aagtattaga ggaaaatcca gaactatcaa
    27181 aggtatttaa gatggttgga gatattttga ctgataaaga aatggctaaa atgaattatg
    27241 acgtagaaaa caacaattta gaacctagag aagttgctcg tgagtttctg aataagaaag
    27301 gtcttttata aattggagga aatataatga cacctattat acaatttaaa aatattaaaa
    27361 aacaatataa tgataaaact ataatagata atcttaattt agatatagaa aaaggagagt
    27421 ttttgactgt aattggttcg tctggttctg gtaagacaac actcttaaaa atgattaatg
    27481 gtctgatttt gccagatgga ggaaatattt tgataaacca aacagatatc aaaaacgagg
    27541 acttaattaa attaagaaga agaataggat attgtgtaca gggaagtgtt ctttttcctc
    27601 atatgactgt tgaagaaaat atttcatatg taccaaatct cctaagtaaa aagaataaat
    27661 tagaagtaaa aagtgctgta aacaagtgga tggaaatagt gggattgcca aatgatatga
    27721 aagtgcgtta cccatcagaa ttgtcaggtg gtcagcaaca acgtgtaggg attgcaagag
    27781 ctttagcttc ttctccagaa attttattaa tggatgaacc ttttggtgca gttgatgaga
    27841 ttacacgaaa gcaattacaa aaagagataa aagaaattca taaaaaaaca ggagttacaa
    27901 ttatctttat tacgcatgat atttatgaag ctttgatttt gggaacaaag actttggtat
    27961 taaatcatgg agttgttcaa cagtatgata ctcctgagaa tattttaaat actcctgcta
    28021 accaatttgt agaccagtta ttgaatataa gaaaatctat aatagatgat gataatttaa
    28081 aagaataaat ttaaatttaa aatcaaattg ttgtgataaa aactattgat ttagagctaa
    28141 cttcaagttg tatacttgaa gtggaaaagt tagattaagg tatagaaggg catttagcag
    28201 ttggctgtag acgaggtaac tgctgaaggt gctttcaata aatggctaga acctgtatct
    28261 gttgaagatt ataataaatt gaaataagaa gctcaatata cagagtagat aggcacttca
    28321 aatagaagtg tcttttgtgt gccataaaat aaatcaaaat attagcaaag aatgtgaaaa
    28381 acaaaataaa ataatatgct atctttaaaa taggagggat gctatatgga taacaatttg
    28441 attaccattg aaaaaacttt agattatata gaaaaacatc ttttagatga gatgttagat
    28501 ttaaatgtaa tttctaaaga gataggctat tcaaaatacc atttacatag aatgtttaca
    28561 tcagttgtcg gatttagtgt acataattat atacaaagac gtagacttac tgaagcagcg
    28621 agaatgctaa tttttacaga aaaaccaatt attgagattg ctttaatctc ggggtatgat
    28681 acacaacgct ctttttcaag agggtttaaa aaaatgttta aacgtagtcc taattttttt
    28741 agaagacatc aagatttttt accactacaa ttaaaatttg atattcataa tagaaaaaaa
    28801 ttacgtggag atatggtttt agatgttaaa aaagttaatt gtgcagatat aaatattgtt
    28861 ggctattctg gtaaagtaag aaaaggattt catattattg gaaaatgctg gagggatttg
    28921 cataaaaata aagagatgat agaaaataag gtagatttag gttttttaat tggagttagt
    28981 gattatagca attttaaaga gattgagaat actcctacat ttacatacat tgcaggtgct
    29041 caagtagcta cttttggcaa attacctaat ggaatggaaa aattcgtatt accgaaaagt
    29101 aattatattg tttttacttt tattgggaaa aatgaggata gtatgcaaaa tgttgtagaa
    29161 tatatctatc aagaatggtt ccctaattca agttgccagt ttaatgaaaa taatttatat
    29221 gactttgtaa aatatggaga aaaaactgat gagaatggtt tgagtgaaat tcagttttgg
    29281 gttccaatca tttaaaagaa ggtattatgt ataaagtaga tggatttata taggcaaagt
    29341 tttacagcag tatgtattta taaataattt atagtttgta ggaaagaaat aattttacta
    29401 taagattatg ataaaataca caatactatg acaaaatagt gttaaataat agatttaatt
    29461 ttgcatagga aaagttctat aatggtgtgt attcataaat aatttataat ctgtaggaaa
    29521 gaaatagttt tactataaga ttatgataaa atacacaata ctatgataaa atagtactaa
    29581 ataatagatt taattttgca aaggataagg tgttttaata aaaatgaatg tagaaaacga
    29641 attacttaaa aacctagata gattgcatac aacagagtta ggcgttgtaa gaattaagaa
    29701 aaatctttct ttggagacaa atgatgtagt gaactggtgt aaaactaaaa tagaatctcc
    29761 aaatgccatt attaatagaa agggaaaaaa ctggtatata agtgtatatg attgtattat
    29821 aacagtaaac gctcacagtt atacaattat tacagctcat aaagaaaaga aatgaattgc
    29881 aaaatacaaa ggtcaaaagc attttccact gtttgtagta cctatgaaga agtatataaa
    29941 ataatattac ttttgaataa taatttaaat tttggaggtt gaaaaaatga atgatactgt
    30001 aaaagaaaaa ttggcagagt cactaacttc tgatactact gatattttac cttatttgcc
    30061 ttatttatta caagattttt gggaattagg aagttcacca gaacaaatgt tacaattatt
    30121 tagaaagcat atacctttta atgaggatac aaaagttttg gatttagctt gtggaaaagg
    30181 tgctgtatct gtgaagcttg caaaagagtt gaatatacat atcaaaggta ttgatttgat
    30241 acctgaattt attgattatg ctagaagaaa ggctattgaa tataaggttg atgctttgtg
    30301 tgattttgaa atgaatgatg ctaatagttc tgtaaaaatt gagcatgatt atgattgtgt
    30361 gatttttgga gctgtagggg atattttagg taattataaa gaaactataa gtaagctatt
    30421 acctacaata aaaagtaagg ggttcatttt aattgatgat gcatatgtag tagataaatc
    30481 tacaaatgaa cagcttaatt ttgagagggt gtatcccact tacaatgagt ggtgtatatt
    30541 gttcaaagaa ttaggtctga agctaattga atgtatacca tgtttagaac ttgaaaatga
    30601 tgttgaagag gaaatgaatt ggattataac tcgtgcaaat gagctaatta aaaaatatcc
    30661 ttctcaaaaa gatatgtttg aacaatatgt tattagccaa aaaaatgaat atactgattt
    30721 acagcaggac cttgttggag caacttggat tttacaaaaa gaataagata aatattagac
    30781 tagataatca atgttatagt gaattatccc atgtctagtg aatataaata acataaaaaa
    30841 ccgtcaagat gaatagttta ttcgtgcttg acggttttta gtatttgttg aagtaaaatt
    30901 ttatttcttt agtatatcac ttttagtcaa tccatttgat ttagcttgag tattccattg
    30961 atgattcccc ataagtttat ataggaatct agggaagtta ggtgaaataa aaatagtact
    31021 tttgttggaa tcatctatat gtatattatc actaagtata ttttttgaaa gttcttttaa
    31081 tgcaattcct aaatcttttt taggtccttt acccaaaggt acggattcta gtgaaccaaa
    31141 catctcccca gctccaattc caataccttg aacccatgta aagttgattt ttttacacca
    31201 atgctctatc atttgaattg ccaaatgatt atgttcacct tcattgaatc cacagttaac
    31261 tattgcatac actggaatag gttttttatt ttgtgttttt aaaaattttt cacatttctc
    31321 taaaaaatga agtaaatgag atggaatacc atctacataa agaggagatg cgattattaa
    31381 tctatcacaa ttaagtatgg cattaagagc gtcaggatac ttagatgtgt tatctacttt
    31441 aaatgtaaaa actttattat cattttgaat aagttgtttt aattcagata aaaaatactg
    31501 tgaattattt ttatttactt ttggactacc atttatcatt actatattca tatggattca
    31561 cctcttatat cttgtatatt ttcatagaaa caaacagagt gttcttttat attgaaattt
    31621 atagcatttc catttactaa tttcgtagca gtttcttttt cagcttctgt aatacaatct
    31681 ccatagaata tagcttttaa atttatctgt ttatcatatc tgcttttatg acgcatcata
    31741 ttatctttta ttacaaagta tggaagaata taattaatgc ttttatcaag tacgtttttg
    31801 acaaaagggc taaaccctcc gtaacagcat ctgcttatta taataattcc atcagagtca
    31861 tttataagtt tacccatgtt gctgtaataa tcttttacaa cacattcaga aggagtttta
    31921 atccagcaac caaaacaacc aatacatttg tggatttctt ttgtgtttgg aagtattttt
    31981 atattgtcat caagtttggg taataacttt tctaattctt tgtcagttaa atcatgtatt
    32041 aaaagcctca taaagtccat ctccttttat tatgtatgtt tacttctaaa aattatatct
    32101 tatagatttt tcagtaaatc tttacaccaa ttaataacaa aattataata gtcaactcca
    32161 taaatcattg ttgcatacat atatgtatat tggtcaacac aatgattttc ttctgcatca
    32221 tttttttgtt tacttaattt atttagaagt aattctactt cagttattaa catttttaag
    32281 tttgtttcaa ttatttcttt gggtagatac ttgtaaaaga atatattaac aaggtggtct
    32341 tgctttgttt tagaaaaatt tataggtttc tttaaccatt caagaaatac ttctttacca
    32401 gtattagtta tggaatatag tttttttaat ttgttaccat ctataacttc gtgatagtgt
    32461 atatatcctt ttgtttctaa tcttttaagt gcaggataga tacttccaaa gctggcgtca
    32521 aaaaagtacg aggtactttc tgacattatt tgttttaagt cgtatccact taattctttt
    32581 tccattaaaa atcctaaaat aatgtattct aacatattac ctcctggtat caattagata
    32641 tatatcgatt cgatatattt attattacag ataataaatc ttacgtcaag atgcttttta
    32701 agataagcta aaaagaatta ggagttattg ataaatatgc aaaaaaatta atataaaagg
    32761 taattaaata tcaataaaga tttaaaaata gttaaatcaa agtaagagtc taatatataa
    32821 aatttatagt aactttatgg tttaaatata aacttgtgtg ctattgacaa agtataaaat
    32881 tagcagtaga ataattgcat gagcaaatag attgttgcga acgcaaaaaa aggacaatag
    32941 atattaaagg agagaatatg agagagctat taaaatgggt ttccattata aatagataca
    33001 atacaaatta tattgacagg aaattggcgg atattggaat caataatagc caatattttt
    33061 atgtattaca catatgtaat aatccaggta ttacacagga cactatattt gagaaaattc
    33121 ttgtgaatcc tagtaatata acaagagctt tggcaaattt agaaaaagaa ggctttataa
    33181 caagagaacc aggtgtaaag gataaaagaa catggcattt atatcctaca gaaaaatctt
    33241 taggatgtta tgatgaaata ctaaaaataa caaatgggta tgttgaggag ttgttgagtt
    33301 catttgagaa agaagagcaa gagttgttta tgtcaatgtt aaagaaagtt gcatttttgg
    33361 ctattgatat gaatgaagaa gcaaaattgg aggcgaagga aaatggaaga aaaatataag
    33421 gagccattgg aatatgaaaa ggtttcaaag ttattaacta agtacgcaat tcctagtatt
    33481 atagcaatgt tagtaagtgc tttatataac attgttgacc aaatatttat tgggcaaggt
    33541 gttggtgtat tgggcaatgc tgctacaaat gtcacttttc cactaacaac aatatgtact
    33601 gcaattgcat tattgttagg aattggagga gcgtctaagt gttcattgga attaggtgct
    33661 aaaaattcag aaaaagctac aaaagcagca ggaaattcta tatgcttgat ggctgttttt
    33721 ggaataagtt tatttatagt agtatcaata tttttaacac cactattaaa attctttggc
    33781 tctacatcag aaatactacc ttatgcacac acatatacaa aaattacatc aattggatta
    33841 ccatttctta taatgtccac tggaatgagt aagttgatac ttgcagatgg gagaccgaaa
    33901 gcttctatgt tttgtatgtt aataggagca gttattaata caattttgaa tccattattc
    33961 atttttgttt ttaatatggg aattgctggg tctgctttag caactgttat tgggcaactt
    34021 atatcttttg gaataagtgt tcattatata aagaattttc aacatattaa attacataag
    34081 aaaagtttta aattagatag tataatttat aaacatatat ttgcgctagg ttcaagctca
    34141 tgttttaatc aattagcaat gactgtaaca cagatagtta tgaataatac attggcatat
    34201 tatggggcta ggtcagttta tggaagtgag attccacttg cttgtgtcgg tatcattatt
    34261 aaagtaaata tgatttttat atctataatt attggtatat cacaaggtat gcaaccaatt
    34321 atagggtata attatggggc tgaaaagtat aatcgtgtaa aagaagcata taagtttgca
    34381 gtgggaagtg caactattat atctgttatt gcattcttat gtttccaatt attcccaaga
    34441 caaattacgt ctatatttgg taacggaaat gagacttatt ttatatttgc agaacattat
    34501 tttagaactt tcttgttctt aacatttata aatggtgttc aaatagtatc atcaactttc
    34561 tttacatcaa ttgggaaatc agtgttggga ctttttactt ctttgactcg tcaaatttta
    34621 tttttaattc cattgatagt aattttacca atgtttatgg gaattgatgg agtaatgttt
    34681 gcaggaccaa tagcagatgc agcagcggca atggtatgta tatactttgc agttcatgaa
    34741 atgaaatcat tgactataaa gcaaaataat ttgatgagta accataataa gattgcagag
    34801 gtataagaca atatttaaga agtatagaaa attaatttaa agaagtataa taaactaatt
    34861 taggaagtat agaaaattaa tctaaagaag tataataaac taatttagaa agtatagaaa
    34921 attaatctaa agaagtgtaa taaactaatt taggaagtat agaaaattaa tctaaagaag
    34981 tgtaataaac taatttagga agtatagaaa attaatttaa agaagtataa taaactaatt
    35041 taggaagtat agaaaattaa tttaaagaag tataataaac taattcagga agtatagaaa
    35101 gctaatctaa agaagtatag taaactaatt taggaagtat agaaaattaa tctaaagaag
    35161 tataataaac taatttaaga aatatagaaa attaatttaa agaaatacaa tacattaatt
    35221 tttaagtaac attaaattaa tgtaagtaaa ataatgttca tatgaagatt taaaggtgca
    35281 ttttctgaag ctaagtatat gttgtagagt gttaggtctc tctatcctgt gaagtttaat
    35341 agctcaagaa aatgcactat ttatgatgat tattttgttt tatttccaat tttggaaata
    35401 actattctaa ttaaatctaa aattatacca taaataagaa aagaaagtat agatgctatg
    35461 taccaataaa tcgaaattga ccctgtaatt gttctacatg ggatttgcat acctgaatcc
    35521 ataaaaacaa aaaaactatc aactctggaa ataatagaca aaataggatt taaacctatt
    35581 agaacaatac tttttgagtc ttgaccaatt tgatgcatat aaatctcaaa taatgcaatt
    35641 aaaaaaaacc aaaatgtaaa agatttaata atttttttca tcaaaatacc tcacataata
    35701 taaattctgt ttgcactatt tgcttactac aactatttat ctccattcag tcacatcact
    35761 taattgtaat ctaggtcttc ttgatggttc ttcatcagga taacccaaag atattgctgc
    35821 cactaattga ctatctgtat ttaaccattc acataattca cgatatgcaa agtatacatc
    35881 acaaatccaa agactaccta gacctaattc cacagctgta agcgacatat tctgaattgc
    35941 agctccaatt gattgcatgt ttgccatttc atagaatcgt tcttcagggg atagcttttc
    36001 taaaggtgat ttacctaata tatttaaaat aaaaattgtt actggagctt gtttcatgat
    36061 ttcaactgtg taatttgcac cagcgatatg ttgacgactt cctggaagaa gcccattatc
    36121 attaatttca ttttgaatac ctttgctcat ggctttaagc atagattctt tttccttttc
    36181 agtgatgaca acaaaacgcc atggttgcct gtttttagat gaaggtgcat ttattccagc
    36241 ttcaattatt ttctctatag tttcatgtga aatgctttga tttttatatt ttctgatact
    36301 tcgtcttttt gatatagaat cacttatcat tttaagtaac ctccgtttat tttttggaaa
    36361 aatctattta atttttattt tctattttaa ttattttagc attaatcaga aaaaaagtca
    36421 ttgattttag tatacaatta ttaatgatta tattaagaat tgtatcaaaa agtatgtgat
    36481 tatactaagg gattatatta aacattatat ttaaaattgt atgaaaggtt gtatttaata
    36541 ttatattgag agatatatat attaggattt atatatattc agatttatta ttatattgag
    36601 ccttatatta aattaaggac tataatttga aaataagcta taaaaactta atattgtttt
    36661 gttcagagat aaaaaaagaa tactttaggt gatttttatt aaaaattaag agtgataaaa
    36721 ttatatactt tataaatgta a
//
LOCUS       DBGJFU010000026        36618 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.026, whole genome shotgun sequence.
ACCESSION   DBGJFU010000026 DBGJFU010000000
VERSION     DBGJFU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.026"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(137..541)
                     /locus_tag="ACOIAS_001277"
     CDS             complement(137..541)
                     /locus_tag="ACOIAS_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HGZ2700994.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPLIYLKYKPDK"
     gene            complement(733..1920)
                     /locus_tag="ACOIAS_001278"
     CDS             complement(733..1920)
                     /locus_tag="ACOIAS_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide family glycosyltransferase"
                     /protein_id="HGZ2700995.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(1952..2167)
                     /locus_tag="ACOIAS_001279"
     CDS             complement(1952..2167)
                     /locus_tag="ACOIAS_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HGZ2700996.1"
                     /translation="MVPPKGKFFFGAVVVGERGQIVIPKEAREVFNINAGDKLLVLGD
                     EERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            complement(2195..2386)
                     /locus_tag="ACOIAS_001280"
                     /pseudo
     CDS             complement(2195..2386)
                     /locus_tag="ACOIAS_001280"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2505..2633)
                     /locus_tag="ACOIAS_001281"
     CDS             complement(2505..2633)
                     /locus_tag="ACOIAS_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2700997.1"
                     /translation="MKDYKYKGYFKGVEIFDNVNDLNLNTLTTVSRFVLVFLFTIT"
     gene            2835..3584
                     /locus_tag="ACOIAS_001282"
     CDS             2835..3584
                     /locus_tag="ACOIAS_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HGZ2700998.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(3750..4115)
                     /locus_tag="ACOIAS_001283"
     CDS             complement(3750..4115)
                     /locus_tag="ACOIAS_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGZ2700999.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(4131..4907)
                     /locus_tag="ACOIAS_001284"
     CDS             complement(4131..4907)
                     /locus_tag="ACOIAS_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2701000.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(5354..6781)
                     /locus_tag="ACOIAS_001285"
     CDS             complement(5354..6781)
                     /locus_tag="ACOIAS_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGZ2701001.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(6842..8770)
                     /locus_tag="ACOIAS_001286"
     CDS             complement(6842..8770)
                     /locus_tag="ACOIAS_001286"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2701002.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(8915..9757)
                     /gene="licT"
                     /locus_tag="ACOIAS_001287"
     CDS             complement(8915..9757)
                     /gene="licT"
                     /locus_tag="ACOIAS_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGZ2701003.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(10104..11474)
                     /locus_tag="ACOIAS_001288"
     CDS             complement(10104..11474)
                     /locus_tag="ACOIAS_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2701004.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQDVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            complement(11513..12184)
                     /gene="pgmB"
                     /locus_tag="ACOIAS_001289"
     CDS             complement(11513..12184)
                     /gene="pgmB"
                     /locus_tag="ACOIAS_001289"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGZ2701005.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            complement(12186..14576)
                     /locus_tag="ACOIAS_001290"
     CDS             complement(12186..14576)
                     /locus_tag="ACOIAS_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HGZ2701006.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVEDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSVGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(14602..16050)
                     /locus_tag="ACOIAS_001291"
     CDS             complement(14602..16050)
                     /locus_tag="ACOIAS_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HGZ2701007.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMIIGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(16453..17340)
                     /locus_tag="ACOIAS_001292"
     CDS             complement(16453..17340)
                     /locus_tag="ACOIAS_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2701008.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(17440..17889)
                     /locus_tag="ACOIAS_001293"
     CDS             complement(17440..17889)
                     /locus_tag="ACOIAS_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2701009.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            complement(17879..19786)
                     /locus_tag="ACOIAS_001294"
     CDS             complement(17879..19786)
                     /locus_tag="ACOIAS_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2701010.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(20054..21949)
                     /locus_tag="ACOIAS_001295"
     CDS             complement(20054..21949)
                     /locus_tag="ACOIAS_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGZ2701011.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(21985..22863)
                     /locus_tag="ACOIAS_001296"
     CDS             complement(21985..22863)
                     /locus_tag="ACOIAS_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGZ2701012.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(23568..25001)
                     /locus_tag="ACOIAS_001297"
     CDS             complement(23568..25001)
                     /locus_tag="ACOIAS_001297"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGZ2701013.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            complement(25024..26865)
                     /locus_tag="ACOIAS_001298"
     CDS             complement(25024..26865)
                     /locus_tag="ACOIAS_001298"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2701014.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(26991..27824)
                     /locus_tag="ACOIAS_001299"
     CDS             complement(26991..27824)
                     /locus_tag="ACOIAS_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2701015.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(28868..29080)
                     /locus_tag="ACOIAS_001300"
     CDS             complement(28868..29080)
                     /locus_tag="ACOIAS_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2701016.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(29081..29497)
                     /locus_tag="ACOIAS_001301"
     CDS             complement(29081..29497)
                     /locus_tag="ACOIAS_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701017.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(29567..29980)
                     /locus_tag="ACOIAS_001302"
     CDS             complement(29567..29980)
                     /locus_tag="ACOIAS_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701018.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            complement(30814..31443)
                     /locus_tag="ACOIAS_001303"
     CDS             complement(30814..31443)
                     /locus_tag="ACOIAS_001303"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HGZ2701019.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(31522..32484)
                     /locus_tag="ACOIAS_001304"
     CDS             complement(31522..32484)
                     /locus_tag="ACOIAS_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2701020.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(33246..>36618)
                     /gene="cbpA"
                     /locus_tag="ACOIAS_001305"
     CDS             complement(33246..>36618)
                     /gene="cbpA"
                     /locus_tag="ACOIAS_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HGZ2701021.1"
                     /translation="EDSNAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNALVV
                     EEATAEDPTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIP
                     KDVKAELIFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRA
                     GKGTTLTIDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSL
                     KVVGNYYSAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDIT
                     INGGTITAKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGG
                     AHGSAFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKA
                     TTFKSLGGAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAP
                     SYTDDQGVLYLWFSDQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYE
                     EFTIDDTPPYLIIKRYDGLPFEEDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMTIS
                     SQRLNEETMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAE
                     IIPADTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGK
                     EYKDPVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGS
                     EDEKKLKLDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWK
                     PSTEGGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGG
                     YDTGNPNLNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGN
                     SKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNG
                     GNSKPDIDTDGDGKPNINIDIDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDID
                     GDGKPDINIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQN
                     VQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT        11785 a         6525 c         3776 g        14532 t
ORIGIN      
        1 taaatactta aatttaattt tgtttaaaca ctagtaagat atactaatat ataaatactt
       61 aaatttagtt ttatttaaac gctctagtga tatttttaaa taaattttaa taaaaatcat
      121 tctattttgt cataaatcat ttatctggct tatatttaag atatattagt ggatacttta
      181 cactttttaa ttcctcatta cttcttaaaa gtttattatt attttccata tcataaattt
      241 gtaacattac tttatttaca tatagagcat aataattatc actataaaaa tattgatact
      301 catatcgagg gtcatctttg taatacactt tgtatagtat tccttcgtaa cttgccttct
      361 tggctttttc ttttgtaata ctttttatat tatcttttga aatatcttgt tttaccatat
      421 acttatcaat atatgtttca gcatctttcc atggtttaat gcagttttta tatacaacaa
      481 accctgttac acttataata cctataatta atactataaa aataacttta tattttttca
      541 tattaatcct ccttcattta ttatttaaaa ctcgatataa tgcactaaaa aaaggatgtt
      601 atctttttta ttaaaatata acatccctca tatttagcat caatatatat gccccaactg
      661 gtaggttaaa agaccccact gtcagtactt tgattaaatt acatgtatgt atatatattt
      721 ttaacaaatc tattatatgt caacttgtct actattacat ataatctgta atattacatc
      781 tgctgctttt tttgagcctc ctgcattata aaagcttttt gatagtttag ttgcattttc
      841 tttatattca ttgtcattta taacttgaaa tactgctttt ttaatacttt ctgacttatc
      901 ctcttttaac ataattcctg ctcccaaatg aacaactctc ttagctacca ttctctgctc
      961 cgaatgttga ggaaataata ccataggtac tccataatat aaactttcat taacactatt
     1021 catcccagaa tgagtaacaa acacatcagt tttttgaagt atagcaatct gttcaacact
     1081 atttttgacc tcaaaattat ttggtatatt tcctaaactt ttaatatcag tacttctacc
     1141 aacagacata atcacatcaa catcagaatc tttaaatgca cttatacagt ttttataaaa
     1201 gctaacattt ttattattaa ttgtaccaag cgaaatatat attagctttc tttttctctc
     1261 tttaggttca ataatcaact ttgatactga tggacctaca aatgaatact tactagagaa
     1321 ggtttctacc attggttgaa attcttttga tgtatataca atggtatccg tatcattatc
     1381 attagaaact atagaaataa aattttttac ttcataccct tttctttgta aaagctttat
     1441 ttttttatta atgcgtctta caccaaaaat catacgtata atttctataa agttttgttt
     1501 catcaacttt gctgtatgtt tattaaatgc aaaagttgtt gtagagcata catatggaat
     1561 attaagtttt tgtgcaaata actttcccca aaaacttaat gaatctgata caatgcaatc
     1621 tggattaaaa tctttaagtt ctctacaaac tttttcatct aaagaaatag tagtatctac
     1681 tatcatctca attaatccag gaaaatcctt accaattttt ttctcatctc cagggagtaa
     1741 ttctggaagg tatttatcac aacaaataaa ttttgctcca gcaccttcaa ttttatcttt
     1801 aaattcatta aaagaataat acaatacctc atttcctcta tccactaatt cacgaactac
     1861 ttcaatagta ggattagtat gaccatatgc aggaatgcta aaaaatacta ttttactcat
     1921 atttcatacc tccttttata atttttttaa atcattcttc tttacgtact actcccacat
     1981 gacctataaa atttatcaaa tcacgttgat gaacaattcc taatcctctt tcctcatctc
     2041 caagtaccaa taatttatct cctgcattta tattaaatac ttctcttgct tcttttggaa
     2101 tgacaatctg accacgttca ccaactacaa ctgctccaaa gaaaaacttt cctttaggag
     2161 gtactataat acctgttttt tcttctgaat gattaactaa gtcatctagt ttgacattgt
     2221 acaattttgc aagtttaatg caactacata tatcaggaat agattctcca ctttcccatt
     2281 ttgctattga ttgtctagaa acattaagtt tttcagctaa ttcttcttgt gtatactggt
     2341 tgatttttct tagacttttt aaattctcat taatcatatt tttcatacct cctatgttta
     2401 taatattgga tttattattt aaattctatc aactaatctt aacattcata tatatattta
     2461 tatactttta ttatataaat ttattattca ggtaacatat ttccttaagt tatcgtaaac
     2521 aaaaaaacaa gcacaaatct actaactgtt gttaaagtat ttaagttaag gtcatttaca
     2581 ttatcaaata tttctacacc tttaaaataa cctttatatt tataatcttt cataagcaaa
     2641 ttattatccc ttctcatata caagtccagt tgatgtcttg ttgctaaatt taatggtaat
     2701 caaattcttt accaataagt ataaaaatta ctaatgtgtt ataattttct taactactaa
     2761 attttacata catttgaata gaatatatca ataaattact ataataaata aaaaatagtt
     2821 agggagatat tattatgatt ttatatttta gcggaactgg taatagccga tatgtggctc
     2881 gtaaaattgc acaagaatta aatgatgaac tgatatcact aaatcaactt ataaaagagg
     2941 aaaaaacaga tgaattaata tcagcagata aaccattcat atttgtatgt cctacttatg
     3001 catggagatt gccaatagta gttacggatt ttataaaaga aacaaaattt ctaggaagca
     3061 acagagttta ctttgtaatg acttgtggtg gagatacagc aaaatccata aactatattc
     3121 aaaaactatg taaatacaag gaatggcaat taaaaggtat ggcagaaatt aaaatgccag
     3181 agaattatat agctttattt tctacaacag acaaagaaac atcaaaacaa atgattaaag
     3241 aagcagataa acaaatatat aggattattt ctgatatacg caatgaaaat gaatttgaaa
     3301 ctattactcc tagtggctta ggaggtacaa ttaaaagtgg tataataaat acagtttttt
     3361 ataagacaat tattagtgcc aaaggatttc attatactga taaatgcatt ggctgtggaa
     3421 aatgtgtaga gctatgtcct ttaaataata ttaatttaaa aaacaaaaaa ccagtttgga
     3481 aaaataactg tactcattgt atggcatgta tttgtggttg ccccactgaa gctattgagt
     3541 ataaaaataa aactcaaaac agagaaagat actatttaga ataatatatt cttaatattt
     3601 aaaccattat aaaatgaact atacccattg ttttatacag ttttacatat tttaaccgtc
     3661 tcctaaaatg ggtatagttc aaaagatagt gtctcaaagt gaatttttta gttcctaaat
     3721 caatcttttt gtattaaatc aagtataatt taatcacctt ttcttatatc attttcacta
     3781 atatgttgta ttccaacaaa tataagattt attagctctg ttagtgactt ccattgtgat
     3841 ttttttgaat caagataata ataatttttc gttccttctc tacgcaaatt aacaatctct
     3901 gcttctttta atatcttaag atgatgagat acagcaggac gagaaagatg tgtttttttt
     3961 gtgatttctc ccactcgtat accattaaaa tcactctcta aaagagcaat taaaatcaat
     4021 tgtctagtct catcacccat agcagagata gctttgcgac attctttaaa tttagacgtt
     4081 atttcttgca ttctttgttg acattctttt tccataaaat ccaccctctc ttataagtat
     4141 ctaacagttg ctacttcttt ctgtgcagta cgtctatatt taacatctgg gtatccaatt
     4201 actaatgttg taagaacatg attactacgt tttagaccta aaagcttttt taattttggc
     4261 gaattatttg ctacatctga aaaatatcca ctaaataaaa cacctagacc ataagactct
     4321 gccattaatg ccatatttga tgctgcaagt gaaccactaa tcttatcttt tgtaattatc
     4381 attattgcaa taggagcatg cttaaagaaa aagttatcgt caatctcaac ttcttttgaa
     4441 tatttaatag ctatatttgc aataggtttt atttttctaa aaaatttcac tgcttcgttt
     4501 tcataaattg actttttatt gtctaaaaca atatatgata catcttgtga atttttagca
     4561 ctaggggtat acctacctgc ctcaataatt tgctttataa tttctgaaga aacttcttta
     4621 tctttaaatt tacgtatact tcttctagat ttaattgcca ttagtaattc atctgaatct
     4681 agctttggct tatttgtaag ttctatagga ggttcatcaa atcctgtcaa ggtaatagct
     4741 tttgtgggac atattgctgc acaatgtcca cacattagac aatcttgttt tttaattact
     4801 gactttttat tttctataat tatattgttt actggacaat catttttaca aagcccacat
     4861 ccaatgcaaa gttctttgtc aacttctatt atatgttggt atttcatatt cattcatcta
     4921 cctttcgttt attagtttaa actattataa cacgtagaca ttttttatat tcattggtaa
     4981 agaatttgat taccattaaa tttttaattt ctctattaaa taaatttttc atagaatttt
     5041 aaaatatcat agtttataca taataaaaaa gacaagctta aaacctaagc ttgccttttt
     5101 catcttttat tacaaagtaa attgcactta cttcccatac ttcttaacaa ttttattttc
     5161 taaaatttta tttgtgaatc atactattgc atcaatacaa tgtaaaataa ttctatattg
     5221 attttctata gttaaaaaag ttaaaaatta tacttattat taatttttat actcttctta
     5281 tttaatacaa agttcttatt taacatagat ttcttgtcta atatagattt cttatttaac
     5341 atagatttct tgtctaatat aaatcttcac catttgaagc aattactttc ttataccatt
     5401 caaaactttt ctttttcatt cttcttagac ttccattacc actattgtct ctatctacat
     5461 atataaatcc ataacgcttt tccatttcac ctgtaccagc acttactaaa tcaataggtg
     5521 cccatgtagt ataacctaat aaatcgaccc catctataga aactgcatca tgcatagctt
     5581 ttatatgttc tcttagataa tcaattctgt aatcatcttc tacataacca ttttcatcag
     5641 ctatatcttt agcacctaat ccattttcta caataaatag tggcttttca tatctatcat
     5701 atacatgatt tagtgtaatt ctcaaaccta atgggtcaat tggccatccc cattgactgt
     5761 atgaaagata tggattttta gcagatgtaa atatgtttcc ttcagccata tcatctgcat
     5821 tttcatcggt tctaacacaa cggctattgt aatatgaaaa tgaaataaaa tctactgtat
     5881 atttattcaa taattcaata tcaccaggtt ccatttctgg tgtaatacct ttttgctcca
     5941 tatactttaa cgcatagtta ggatatttac ctttagattg tacatccaca aagaagtaat
     6001 tttctctatc taactttgta gcttcccata catcattagg attacaacta taaggatata
     6061 cacttccagc tgcaaacata catccaatta agttatttga atcaacttca tgtgctattt
     6121 tagttgcaat tgcactagct acaagctcat gatgggcagc ttgatatttt atcttttcaa
     6181 catcttcacc ttcttcaaag caaataccag cacccataaa tggagcatgt aaaatcatat
     6241 ttatttcatt gaaagttagc cagtacttca ctaatccttt atatcttcta aagataacat
     6301 tgcataattt ctcatagaaa cctatcattt ttcgattacg ccatgcacca tactttttaa
     6361 ctaaatgcat tggacaatca aagtgtgtaa tagtgactaa aggctctatt ttatattttt
     6421 tacactcctt aaatatattt tcataaaatt gaagtccctt ttcatttggt gtttcttcat
     6481 caccttttgg aaatattcga ctccatgcaa tgctcattct atatacttta aatcccattt
     6541 cagcaaataa ctttatatct tctttataat tatgatacat atcaatacca gttttagctg
     6601 gataataatg ctcatcatca aaatcaaaca tttttagttt ccctgtaata actgctgttc
     6661 tatcttttcc tgttggacat acatctacat ttgccaatcc tctttcgtct tcattgtatc
     6721 caccttcaca ttgattggcc gcagtagctc ctccccataa aaaatcattc ttaaaactca
     6781 tagtcaaagc ctcctaaaat tataaccaaa gcactagatt atgctaatac ctcaatcttt
     6841 actaaaataa gactgtaatt agattgtctt ctaattcaat atttttactt tctgtttcta
     6901 ctacatctaa aaattgtgat gaatttgtaa taacaattgg agttacagta ctatacccca
     6961 actcttttat tgcataaaca tcaaagttta atatagtttg accttttgta attctatcac
     7021 cttgttttat acatgcctca aaacctcttc cctctaattg aatagtattt aatccaatat
     7081 gaataagcac ttcaacacct gtatcagaaa gaatccctat agcatgtaga ctaggaaata
     7141 aggttgtaac taccccatct actggagcaa ctacttttcc atcactaggt attattgcca
     7201 ccccttgacc tagtacacca gatgcaaatg ctgcatcctc tatatcactt aattttaata
     7261 cttcaccttt tataggactt gttactattt ctctttctaa tttagtagac ttgacttctt
     7321 ttatttcttc tttaccctta tttcttactt ttttagactc ttctactttc tcatctttat
     7381 ataaaatcat agttaataca aaagctacga ccattgatat agctatacca gaaagagcta
     7441 ctattaagtt acccattccc ccatctggtt caatcattgc tggaagttca aatattccca
     7501 ttcctccagt tataaatttt ctaagattaa agaatccata gaaagcgcct ccaataccac
     7561 tagcaataca acttattata aatggtttct ttaaaggcaa taatattcca taaattgctg
     7621 gctctgtaac tccaaatata ccagatataa agtttggtat tgccatctct tttagttttt
     7681 tatctttagt cttgaaaaat attgccaaca caactgccga acttgcaaat gtacaggcaa
     7741 agaatggcat cataacatta tcatatccta aagtcataac attgtttata tatacaggaa
     7801 taaatcccca gtgcatacca aaaataacaa gaatttgcca agtaaatcct actatagcac
     7861 ctgccaatag tggactaaaa tctcttattg aaattacaat ttgtgaaata agtgtagaac
     7921 caaatgttgc tatagggcct ataactataa gtgcaacagg cactgttatt aataatgtaa
     7981 gcattggcac aaagaaaaac ttaactaaat ttggaacaca cttattaaat aatttttcac
     8041 acctagaagc aaaatacact ataaatataa ttggtacgac tgttccagta taatcaattg
     8101 atacaattgg tagtccaaag aaatcaatat atactggtga tgcaaacatt gttcctttaa
     8161 ataaagtgta catcacatct gctcctgctg atatactgct accttgtata gcaggataac
     8221 atactgcagc tcctagtgct aatcccaaca ttggttttaa tccaaacttc ttagctgaag
     8281 tatatcctaa gaacaaagga aggaatgtaa ataatgcatc tcctgctgca tttattacca
     8341 tataaccacc agatacatca ctatataatc ccattgctac aaataatgca ttaaatcctt
     8401 tcaacatacc acaagctgcc attattccta acactggctg aaaaattcct gatattatat
     8461 ctataccttt atctagtata ttacctgatt ttttagtttc tgaacttgta cttgaatcat
     8521 ctaaaccaat tatttcaact acatctttat agacagcttc aacatgattt cctataacta
     8581 cttgatattg tcctccactt ttcattacag taacaatacc ttccatgttt tttaatacat
     8641 tgtcatttgc tatactttca tcttttaaag taaaacgtaa tcttgtaata cagtgtacta
     8701 acccactaat attttctttt ccaccaacat tgtttactat ctcttgtgct aattgtttgt
     8761 attttcccat ttttctcctc catatttcgt ttaaattaat tttttgaaat ttgaaggttt
     8821 ggccaactca atgttaccat ccaaccataa gtataatttt gttagtatca ctcctttcaa
     8881 tcattcttaa tatactactg aaaaattatt tcacttacat atttgagtct ttaactactt
     8941 tagctaaatg tattgttaaa tatataagct catcatctgt taatatgtag ttatattgtt
     9001 ttttaatgaa atttttaatt ttaactacac attcaaactc tttattatat ttagatttaa
     9061 taatgcttag taattctttg tctgtctcac cttcatgtgc actatctaaa agcaatcttt
     9121 gcgcaaaaaa ctttaaatgc attataaatc tataataaaa cactgattct tcatgaaatt
     9181 ctatcctaaa atgataccta actatgctaa gtacttcttg tattaacttc gtaatatcag
     9241 atgccaaagt atgaccttca cttaactgag cattcactat atgaaaggct ataaatccag
     9301 cctcatcttc tggtaatttt atacctgttt tgttttcaat aatatctagt gctttcattc
     9361 caatttcaaa ctcttcttta taaaatcttt taatttccca aaggattgca ttttttacat
     9421 ttattccttc tttcatcctt tgaattgcag aatatgtatg gtcacttaag gatatgtaaa
     9481 tactttcatt tagcttcttg tttaacttta tttgtgcgta ttctataatt tcacttgata
     9541 atttcatgtg ttctatagga atattattga gtaattcttg tagtttgttt gatatttctt
     9601 tatttgatat tctaaatact ttatttactt tagttttatc tattagattg ccttttgatt
     9661 tttgataacc aattccttta cccataacga taacttcttc atcattatca tctaatgtga
     9721 ttacaacatt attgtttaat atcttattaa ttatcatgat tatcctcctt ttaagcataa
     9781 aaaaagctta tccataataa tgggcatact ccttcctagg atactgctat tattaatgaa
     9841 taagcttagc ttgtacttaa acaattacta cctaattcca attaatatga taaaacatca
     9901 gacatatttt gtcaactttt tatttccata tacttcatca atcttttatg ttgctttttg
     9961 tcagttctaa taattattct tattaatatt aacttatttc tttgcatcaa tataaataca
    10021 aaacaagtta tagtcaataa taataaaaat tctattaatg taaatagtta atattatact
    10081 tcctctacta cattatctgt actttattta acattttttt caactccaaa aacatatgtc
    10141 ataataaatg gagcaaccaa tgctataaat ataactataa gtccatttac tatatttgaa
    10201 atcccctttt cccctgataa tccaagtact gccattatac tattttgtgg aacatataaa
    10261 gtcacatgag taattcctgc aaataaccct gcgattcctg ctcctataga tgctaacata
    10321 gcaggttttt taagtttcaa tgtaactcca taaagagctg gttcagttat acccatgata
    10381 gctgatatcc atgcagctga tgctaactgt ttcatcttac tatctttgct tcttaatgct
    10441 acagctaatg ttgcgccacc ttgcgctaaa ttagcacaaa cttgagatat taatattagc
    10501 gattcttgtc ctgtagttgc aaatgctgat atgaatatag gagtcaatgc atgatgcatc
    10561 cctgtcataa cgataaatgg cattgcagct gctaataaac aaaccattat aaatcctaac
    10621 cttccttgta gtaaatttat tatactagct aatccctctc ctaagaaatt tcctattggt
    10681 ccaattacta ttaatgctat tggcgcactt attaataata ctaaagttgg atttaaaatt
    10741 gtttttaaca tgctaggtgt taatttatca atgaattttt ctatatatga catcatccat
    10801 actgttaaaa gtgcagggat aacagatgct gcatatataa catcttgaat tggtactcct
    10861 ataaatgata tagggtcccc acttgatacc catgcagaga aatttggatg taaaaacaca
    10921 cctactacta taactgctat tgatgtatta acattaaatt ttttagctgc atttattgca
    10981 agtattatag gcataaagta aaatgctaca tctcctatta tatctaatac tctatatgta
    11041 cttgaatttg atgatagcca attaaaagtg gtgcaaagag ataatataac ttttatcatc
    11101 ccagatgctg taagggctgg tatcataggt gtcatacaac cagttattgt atctattata
    11161 ttatttaaaa tactattttt tttattttta ttttttatat cgcttttaat atctcctgtg
    11221 gtatttatcc ccttatttat tattgcatta aatacaacac ctacctcatt acctattatg
    11281 acctgatatt gagttgaagt tgagtttact ccaactacct tatctataga ttttaattta
    11341 tctgcattga ctttcttttc atcttttaag atgaacctta atcgtgtcat acaatgagtt
    11401 aagtcaatta tattatcctt tcctccaagt ccatcaatta tcaattgagc tacttcttca
    11461 tatttattct tcatacctta tcctctcttt taaaataatt tttaatataa ttttaatcca
    11521 aattaatctt tttactccaa gcttgtaata gtgtattgta atttaataag ctagtatctt
    11581 ctacaatatg gtcacatttt cctagtactt cttttttccc cacaccaatt gatgtcatat
    11641 tagcagcatt tatagcttta atacctgaat aagaatcttc tattcctaca caatcatatt
    11701 gttttaaatt tagaatcttg ctcccttcaa taaaaatatc aggatatggt tttccccttt
    11761 ttaataaaga tggatttact atacaatcaa attcatcttt tattcccaaa gattctaata
    11821 tcctaggtgc attcttacta gcagatgcta ttcccaattt cacattattt tttttaagtt
    11881 cttttataag ctcatttatc cctggtaata tatctttata tgttaatgag tttattaact
    11941 ttatataaat gttatttttt tcttcacaca cctttaactt ttcctcttta gtataactat
    12001 ctactttatt agcagataac aatattttat ctaatgattc ttccctgctt atgcctttta
    12061 atttttcatt aaaagtttca tctatttcta tatctagcga ttttgctatc tcatgccaag
    12121 cttcaaaatg atacttagca gtatctgtta taactccatc aagatcaaat aagactgctt
    12181 tcatattaat tactcccttt aaaacaatta ttaacttcta ttatgttctt tgataatttt
    12241 atttctttat tatcatgtaa tatggttata tttctacctt tgattaactt ataataagat
    12301 ttatattctt ctatatttac agaaactaaa ttatctctaa ataatatatt aaatttaata
    12361 tttttccagt gtttaggtat ccttggttca aaacttaatt tatcattttt tattttcatt
    12421 cctgcaaatc catatactaa actcatccat gttccaccca tattagcagc atgtattcca
    12481 tcacttgtat ttttttgtag gttattaata tccattaatg ctgtgtccat aaaatagtta
    12541 taagcttttt catgatttcc aatttcactt gccatcattc caaatattga ccttgacaaa
    12601 gatgaatcat gagtagttat tttttcatag tattcaaaat ctctcttctt ttgttctaag
    12661 ctaaactcat cagggaaaag caacgctgaa agtacagtat ctgcttgttt atttacttga
    12721 tatctatata ttgttaatgg atgataatga agaagtaatg gatatttttc ttttggtgta
    12781 tttttaaaat cccatactga tttttctaaa aatgaatcgt cttgtttgct tattttcttc
    12841 tcttcatcat atggtaaata catattttga gcggcattta tccagttttt cacttcatat
    12901 tcttgtaatt taacttttct aaatacttca tttgaaaata aattatgctt aaatttatct
    12961 accatctgta ctgcatatat taaattatac ttagccatta aattagtgta gtaattattg
    13021 tttactattg ctgtatattc atctggtcct gttactccat ttatacagaa tttattattt
    13081 tttgattcta tataatctcc aaattcaacc caaaatcttg ctgtttctat aagaatttct
    13141 aagccatttt gcatcataaa ttcttcatca ttagttgctt caaaatacgt tctaatacca
    13201 taagctatat ctgcatttat atgaaactgt gctgttcctg ctggataata tgcactgcat
    13261 tcattaccat ttatagttct ccatgaatat aagcaacctt tatttattcc aagttctcta
    13321 gctctttctc ttgatgcatc taagattgag tatctataat ctaataaagc tctggcaatg
    13381 gatggttgag tatatataaa aaatggtaat atatacattt ctgtatccca gaaataatgc
    13441 ccctcatagc catcacctgt aagacctttt gctgatatat tagtctttcc atctcttcct
    13501 actgattgat aaatatgaaa taaattggtt tttatcccca gttgaagagt tttatcgcct
    13561 tcaatctcta catcagaggt tgtccaaaac tcattcattt tctttctctg aatatttttg
    13621 actttatcaa atccaatatt acatgcttct cttaaaacca attctaatga ttttaaatgt
    13681 gcttctgatt ctaaacattt taacgactcc agacattcta atgattttaa acgttcttca
    13741 tttagattta aatcagaata tactctacta tagcctacaa caatttctaa atttaacctt
    13801 tcatcttttt tggcatagac ttctatttct cgctcaatac attcatctga aacattatag
    13861 gtctcatttg taatatttcc acaagtaata tatgaatcaa acccacacca taaccctaat
    13921 ctacttcttt ctgtaactaa attcataaaa tacttattgt tatatttttt tacattaatt
    13981 ttaaattgct tcttatttat agatgccact cttgggtcct ctactttttc acaattaaac
    14041 ttctccatac tattttccat tttattttta aattttattt ttccattaaa attcaatggt
    14101 gttattgtat atgaaatcat cattaattct tctatatctt gtgatactaa tctttctata
    14161 gatactttaa cttttctacc acgagagttt tcccatataa atgaacgttt aagaatccct
    14221 tcatttaaat ctagtatcct agaatgttca cttgtaatac cttttgataa atcaaagcaa
    14281 tctccttcaa tagagaaatt tactatatga gcattaggta acttacagat agtctgattt
    14341 tgttttgcat agccataagc gctttctcca tatgtaattg gagattcttc ataaaatcca
    14401 tttacatatg tacccatatt atctacatac tcattattaa aatcagactc ttcaatattc
    14461 cctctaactc ctaaatgtcc attagctagt gtaaacagcg tttcattgga ttttattgta
    14521 tccttactaa acccttgctc tataagttgg tccttagtag taaatattaa actcataata
    14581 acactccttt cttaaaatga attatgcatg agtttccatg tcattaaata aaatttctcc
    14641 atctgtttct attacttttt tataccaaaa agcactttgt tttttagttc ttttaaatga
    14701 gcctcttcca tagtcatcag aatcaacatg aattatgcca tatcgttttg tcatctttcc
    14761 ttcacttaca ctggttacat ctatacatcc ccacataaga tatcctataa gctctattcc
    14821 atcttcattt actccaataa tcatattttt aatatgttct ttcatgtaat ctatacgata
    14881 atcatcaatt atttttccat cttcatttac aatatcttga gtacccagtc cattttctac
    14941 tataaataaa ggtaactgat acctttcata taactgattc atagttattc ttaaccctac
    15001 agggtcgttt tgccatacac tatttgattt tggaagatat ggatttttta tggtttctgg
    15061 tccgtttgcc tttgattttt ctaaaccttc agctctagct gaactacaag cactagaata
    15121 atagctaaaa gatacaaaat ctacagtatt atccttaagt gttgataaat cttcctcttt
    15181 tattattaat tttatttgat ttgattttaa ataataattc ataaagcttg gatacacacc
    15241 cctaacttga acatcagaaa aaaacaaatt atgcctttca tgctctaaag ttgcaaatac
    15301 atcttctggt ttacatgtgt atggatatgt ttttccagca gctaacatac acccaaactt
    15361 aaattcttta tttatatacc ttcctatttc tatagttttt gcattagcta ataattgatg
    15421 gtgtgctgct tgatattttt tctgaagttg attttcatca gctataaaag ttaacccagc
    15481 acctatatat ggaatatgca ttatcatatt catttcgtta aaaggaatcc agtatttgac
    15541 atcatcttta tatcttttca tcaaaatttc agcatatttt acatataaat taaccactct
    15601 tctatcagcc cacccagaat atttttcaac tagagaataa ggtatatcaa agtggcttat
    15661 cgttattaat ggctctatat tatatttttt taattctttt attatattat cataaagttg
    15721 taatcccttt tcatttggtt cactttcttc tcctgttggg aatattcttg accaagatat
    15781 agaaaatcta aaacatttaa aacctatttc tgctaacatt gctatatctt ctttgtaatt
    15841 ttcacaaaat ttaatcccta tatgacttgg atagtattga ttattttcat ttaaaatatt
    15901 atcctcatct aaacatacat cttttaatct cttatcattt aatgggagtt tatcaaactg
    15961 agataaacct tttcctccat ctaaataaaa tccttcactt tgattggctg aaattgcccc
    16021 tccccataaa aaatctttcc ctaatttcat gcatctacct ctttcacaat ataaagtttt
    16081 ataatttcta tctcatattt agttataaga tttttttcat actatacctt ttaaataatt
    16141 caatttaaaa atccaattat attttttact cactataatt aatattgtct attttcaata
    16201 ctttcatatt aaatatcaat attagatatc tatgtatata attatatata tagcaaatac
    16261 atttttatat cctcattatt gttatcttat tctcatatat tgctgtatat ttatatataa
    16321 aaggatttat ttcgaaatag aggttgtatt gagaactaaa atttagactt tataaatgaa
    16381 tggaattttc attgttgaaa tactgaaaat atatttgatt tcatataaaa gaaagtgcct
    16441 catgaactaa gttcattttg agacactccc ttttttttcc aagctattat atcttttttt
    16501 aaattctaat ggagagactc caattttttc tttaaatact ctgtaaaaag tttttgtact
    16561 tccaaaacca acttcttcaa ccaaatctac aatggatata ttgttattaa gtaatatcca
    16621 ctttgctttt tctaatctat attcatttaa aaaagttata aatgtttttc ctgtattttt
    16681 tttgaaaaat ttagtaaaat aatatacact aaatcctact tcataagaca catcttctaa
    16741 ttttataggt tgtttataat ttttttctac atatttgaat atattgttca atctctctaa
    16801 tatctcttta gagtttattt catattctac tgtattttct tcaatttctc taggaatctg
    16861 tctatataat ataggaatta aattatacat tttacctttt attgcataac tatatccttc
    16921 tattttgtat aggtcttcat tgtagatatc tatcaatata tcgaaaactg actttcttat
    16981 ttttgctgac caatctctac tacatttttt tatactactg aataaattaa ttaagctttc
    17041 tttttcctca tttaataaaa ttaaatcatt aaaaaaagat atatcaaatt gaaatactaa
    17101 tctttcactt cccggtgatg gcagtatata atgaatatct cctcctttta ttatcactat
    17161 ctcgcctttt tttaattgca taggcttgtc atttataaat agatttacta caccttctgt
    17221 tataagtaat atttctattt ccttatgcca gtgtggtgtt gttaatactt ctccttcatt
    17281 tgatagacta cgaaaaggaa attcatgtat taatttaggc atttctaaat atattgccat
    17341 atatatcctc tcctctctag tgtctaaaaa ttaatttata ctatttaaaa gaatacacat
    17401 tttattttaa aaacctcaaa tttataattt atacaatacc tacacttcat aaaatatatc
    17461 taatatttct ttttttgtat ttgcatttaa aagtctttca cctatatttt taccacctaa
    17521 aatgtaagct attttagata ttaatatcat atcttcattg cctccagatt gtggcatagc
    17581 aaacataatt attaccttta caggtaagcc atctatactt ggccaatcta cacctttttt
    17641 agatttacct attactatgg atgccttctt tacatatttt gtttttccat gtggcatggc
    17701 catttgataa cctatgctag tagagcctat tttttctcta tgttttaatg cctttataaa
    17761 cttacttttt aaagttatac ttcctgtatc gtataattta tctactaatt cctgtattat
    17821 gccttctttt gtagttgatt tcaaatctaa aataatgctt tcttctgtta atatttctct
    17881 aatttccatt tgttttctcc aatagattat tcttaaataa tattttaata tcctctttgc
    17941 tcttgcaatc tattatatag tttatatagt tcttttgcct atataaattt gatatttcca
    18001 ttaaaatatt ctttaaaaaa ctttgattac taatttctaa ataaatatca atcgttctag
    18061 taacattatt gatattcatt atccctttat ttaatttact ttgcttatgt atatatatat
    18121 ttaaatcttt tcctaatgaa atagattcat atttactccc taaattcaca cctattaaac
    18181 tttccatctg agctacatca aaattaaaat atatattatc ttctaaatac attcctaata
    18241 agtttgtatc taagttcata ctttttttag gtttagaaag taaagcattt tttatattat
    18301 taatatcatt ttccattaat agtactgata caactatatg taatttatct ttcaaatcta
    18361 agtttacagt agatattaca aagtctacat catctaaatt tcgcttactc aactgatgca
    18421 taggaactat atcaactata gtccattcag gaaattctct ttttaatctg gctgcaagga
    18481 gctgagatgt tccatatcct gtatgacaaa ctactattac tcttttacta ctcatatttc
    18541 tttctattgc cgcttgaaaa tatgtggcaa gatacgaaac ttcatctata ctaatattct
    18601 tcagattatc atactctgtc ataatattta tagtcatcat acataatccc agcacatctg
    18661 aaaatcgctc ttgtatctct cctaacaacg ggttttttat ttggatatca tatcttaatc
    18721 tgtttaacat aggccttaca tgagatgcta gacttttctg tagtaagtca tcatttttga
    18781 gatttatatt taaaaatcgt gacatattct ctatcattat agatgaaact ttttcacttt
    18841 cacttaatct atctgattca tcattgtaac tactattatt ttcgctacta aatccagatg
    18901 ataccaaaaa aatatatatg tacataattt catctttcgt tatttttact ctgtatctat
    18961 tttctatttt atctataagt gtactgacat ttttatatac taactcgtcc aaattatcta
    19021 cagaaatttc tctttcttcc tgagattcta tttgattacc ttcttcgatt ctctttaaac
    19081 atataagtat atgggtaatt aaatttatat agtagggctg atttatagta tatcctaaat
    19141 tgtattctaa ttctgtaatt atagtttcta caaatataat agaatcaata tcaaataaat
    19201 ttatcaatgc actaaatgta gcagaatcta atcttgttaa ctcagatata tcatgttgtt
    19261 cataattttc atctaataaa tcatccatca ttttggcaat cgatttcctt atatccactt
    19321 ctttacctat aatcttagtt ccttctaaag ttttattaag ttttaaattg tattctttta
    19381 tccatgcctc tatatattta aaatcattta ctatagaagt tttacttaca taatatttat
    19441 ctgctaactg atttattgaa gttatattat ttgaatttag taataaatac tttaaaatct
    19501 ccattcttct ctgctctact gataaagatt tattttcctt cgattgtata tttaaactat
    19561 taattaagtc tatattcttc tttgctaaat cttctattaa tattccatag cctcttttta
    19621 tatcaatttt tatattaaaa gtttctaaat atttctcaat catcttcaaa tctttttgta
    19681 aagttttatc agatacattt aatttatctt taaagtattt tataggtcta tatttatttt
    19741 cgtttagtat caatttaaga aattcaattt gcctcgacac aagcatactt agccctccct
    19801 acctatgttt atatatttct atgtttatat taagtactat tttaacatat ttactaacta
    19861 tgtaaaatta ctaagatact tctgtatcat aaaagaattt atgatacacc ttactaaatt
    19921 gatataatag ataatataat ttcaaaataa aaattaagga tatttagaag tagttaactg
    19981 attctaaata tccttattat tattctatat acttgttgtt attccattaa ttatttttac
    20041 tattatttat aatttaagca ccaagtaatt caacttcact attttcactc cttatagtag
    20101 gctttgttac ccctataatc aatgctgtta ttactgtacc aatcaatata caagctatat
    20161 atgcaactgg tgcactacat gcaaatggta ttacaaatat tcccccatgt ggtgccatta
    20221 aagttatatc taaactcata catattgccc cacctataca agaaccaatt atatttgctg
    20281 gaatcattct taatgggtct gcagctgcaa acggtattgc gccttctgtt atgaaagatg
    20341 cccccattat agcagttggt agagatgact ttctttcttc tttagtaaac ttatttttga
    20401 atattaacat agctaatgat attgctaatg gaggtaccat tcctcctacc attaatgctg
    20461 ctgatggttc aaatatatta cttgaaaaca tagccaatcc aaaagctgaa gctgttttat
    20521 taataggtcc tcccatatct gatgccatca tccctgctaa tattgcacct aataatactt
    20581 tattagttcc agacatactt tgtaaccaag attgtaaagc ttcatttaac catgatgctg
    20641 gcgtattaac tacaaagaac attatagctc cactcgtaaa tgcaatcaaa agtggacata
    20701 tcaatacagc atttactcct tttaatacat ctggtaaatt ttctgttatc ttcattactc
    20761 ctaaagctat atatcctgtt gcaaaaccag ctaccatacc acctataaaa cctgaacctc
    20821 caataactgc cataaaacca gctatcatag caggagcaaa tccttgtctt cctgctattg
    20881 atttagctat aaaaccagca agtatcggta taaataactt aaatgctgaa tcgcctccaa
    20941 gttgtccaaa gaatccagct atagggttgt atgtttcatg agttgggtct gatgcaaata
    21001 taccaaacat aaaagctatg gctataccaa ttccacctat cactacaaat ggaatcattt
    21061 cagatacacc actcattaag tgcttataaa cacccttttt ctctgaccct tgaagcattt
    21121 cttttgaatc ttctaaagtc tttccacctg gtttaatttc tttagccatt ccattttcag
    21181 cgtttataat taaattttta gctaatgaaa cattcttagc atctactact ttcttttgta
    21241 gcactttctt tcctataaat ctactcagtt gaacttcttt atcacaagct attattacgt
    21301 attcagctct ttttatatct tctttagtca acttattttc tacacctata gctccatgag
    21361 tttctatttt aacttcatat ccaagttctt gacctgcttc ctctaatgct ttttttgcca
    21421 taaaagtatg agctatacct gttggacatc ctgttatccc aactataaga cctttatttt
    21481 gattattact aaaatctcct gattctgcag ctttattatt aaatatactt aatatttcgt
    21541 atggagtttc tgcacttagt agatgcttaa gaaaattttc ttccattaga tatgtagcta
    21601 gtttagatat aatcttaaga tgttcatctg aacctgattc tggaactgct atcataaaaa
    21661 atgacctaag tttttgacca tcattatctg taatctctct atctgttctt aaatacatca
    21721 ttacaggttg atttactaca tttgacttag catgaggtat agctattaaa ttacccatat
    21781 tagttgatga ttctttttcg cgtttattaa tatcagctat aaacttttct ttattattta
    21841 tataatttcc atggtacaaa tgatttgcca tataattaat tacatctttt tttgtttgta
    21901 attgtttatc tagtacaata caatcgcttc gtagtatttt acctatcata aattctcccc
    21961 tccttttaat ttgttacctc ataattaatt tttattttta taaaagcact ttaacttatc
    22021 tatagcattg aatagattca ttttaaactc tataacttct cttgcagatt ttatacatgg
    22081 tggatatata gtatctggct catataagct tgggttttgt tctaatagtt ctctgcactt
    22141 cttataatat gccgacttaa catcactcga aatatttatt ttacaaactc ctagtttaac
    22201 tgcttgtgct atttcttcat ctggatttga tgaaccacca tgaagaacta aaggtatatc
    22261 tactacttct cttatttctt ttaataaatc tagtttaagc tctggtttaa aaccttttgg
    22321 atatatacca tgtgctgtac caattgcaat agctagtgtt tctactcctg ttttttcaac
    22381 aaaatcctta gctaattttg aatctgtata tattatttca tcagtaccac cttcacttga
    22441 tgttcctgtt gttcctattg ttccaagttc tccttctacg gacacattta atccttttgc
    22501 tatttctact atttccctag atatagctac attctcttca tatggtaaat gtgagccatc
    22561 aatcattact gatgtaaatc cacatcttat tgcccttaat atttgttctt tattttcccc
    22621 atgatctaag tgtattacca taggtacttt actcttattt gcttcttcta tgcaactagc
    22681 tacaaagcta tctcctagaa aacttagctc atttgggtgt attgcaagta ttactggtgc
    22741 atttttttcg ttagcactct gtactactgc tttaagtatt tgcccacttc ctatattaaa
    22801 tgctggtaca gcaaattgat tttcttgtgc cacttttagc atttccttca tatttattaa
    22861 catattgtac ctcccgatat tttaaatttt aggttgaatt tgcttcttgt taattttgtt
    22921 gttgtttata tttaaataat atcagggtaa tgaagatcag aaaataacaa tcgttttcct
    22981 tagtgaaaag gaacttttat tatacactct aagttccttt tcactaaata atttttagaa
    23041 gtctgcatca tagttaatta tatttctcat atacatcttc tctttttaag tgttttatgc
    23101 tttagccttc ttaactcttt ccaataaaca attagatagt ttagctataa atttaaagat
    23161 attcgtatcg tagttagcac tatattggaa tcaaaaaaat taaaaagata ctagatagca
    23221 gataccaatt attaatacac cataagtcat agatacttct atcttaaatt cttgcatatt
    23281 agttacaata gaaattaaat aggactatta taaaaatcgt aaggtaagac tgacatttat
    23341 tctccaacta tacaaatgaa tactaatata gctccttcta attaaatcat actatataaa
    23401 tcatatatat cccaattttt aaccttacct ctttatattt actttttaat aaaaaaagat
    23461 attgactaaa tagtcaatat cttttagtgg atttaaatac ccacgatttt aatctaattt
    23521 ttaatggttt ccttaacata atcttataaa tattaaaatt tatctaccta taaaatttct
    23581 ccattaaatt ttattacatt tttataccaa tcaaaagatt tctttttata tcttttaaga
    23641 gtcccacttc catcattatt tttatctaca tatataaatc catatctttt tttcatctct
    23701 cctgttgtaa atgatactgg atctatacat ccccaaacag tataccctat aacatcaaca
    23761 ccatcaattt caatggcatt tttcatttcc cttatatgac tagccaaata ctctattcta
    23821 taatcatcat ctacagtatt atctgatttt aatacatctt ctgctccaaa tccattttca
    23881 acaatgaata taggaagttc atacttatca tatagttgat taaggtaaaa ccttagacct
    23941 acagggtcta tattccatcc ccaatcagaa atttttaagt atggattttc aactacatca
    24001 gcctttcctg ccgagtcatt acctgttgtt ttcatagttt tatcagctgt tactacagta
    24061 gttaaataat aactacatcc aataaaatca actattcctt cttttaggat ttttttatct
    24121 tcctctgtaa tatctaaatt ataatttttt ctttcccata attttagtgt atgtcttgga
    24181 taatgacctc ttacatgtac atcagtaaac ataagttgtt ctctattaga ttctacaaat
    24241 ttaatcatat cctctggatt gcaagaataa ggataacttg gcatagctgc caccatacat
    24301 ccaattttaa actcagggtt tatttcatga cctatcttca caacctttgc acttgctaca
    24361 aattcataat gaactgcttg atacataact tcttctttgt cttcctgctc ttcatagatt
    24421 attcccgagt tagtaaatgc ataaatagga tttgtagtta tactttgatt atttatttca
    24481 ttgaaagtca tccagtactt aactttatcc ttgtatctat tcattaccac ttcacagaag
    24541 tttacaaaga aatctattaa ttttctattt ctccatccac catagttttt tactaaatga
    24601 taaggcattt caaaatgact taaagttatt actggctcta tattgtattt taataattca
    24661 tcaaataaat catcataaaa tttcaatcca tcttcatttg gagttgattc atcgcccttt
    24721 ggaaaaattc tagtccaagc aatacttgtc ctaaaacact tatatcccat ttcagcaaat
    24781 aatctaatat cttctttata attttcataa aaattaatgg cttcatgatt aggataattt
    24841 aaaccctcaa ttactccatc tgttatcttt cttgaaatag tatgagtacc tgcagtcatc
    24901 acatctgcaa tacttattcc ttttccacct ttattatatc caccttcaac ttgatgggca
    24961 gcaactgccc ctccccataa aaattcttga ttagatttca tagaaatatc tcctccagta
    25021 taattaataa acaactttta ttacaggcgt tccaatttta atatgtttat tttctttaac
    25081 tattacatct gctaaatcat ttgtgttagt tagtattatt ggtgtaacaa tactataccc
    25141 ttcaccttct attttatcct tatcgaaaga aacaatccta tctccttttt taactttatc
    25201 tcctgcattc atatgagtag tataatactt tccatctaac atagtagtgt taagacctac
    25261 gtgtattaat atttctacac ctttatcaga ggtaattcct attgcatgct ttgtaggaaa
    25321 aatagttgtt attgttccat ttacaggaga cacaacttct ccctttgaag gtattattgc
    25381 catgccatta cctatagttc ctgatgcaaa cacttcatct tctacttcag ataactctat
    25441 ttgtaagcct tctattggag ataatatctc ctctatacca tcatcttcat caaaagcaac
    25501 ttcttcatta ccttcttcac attcttcttt actcaatcca aagaaatatg acaatactgc
    25561 tcctagcact aatgctgttc ctattcctaa tatatatcct aggaatggtg tataaactgg
    25621 cattgagaat atattatgaa atacatatgc attacaagta acaccaaata caccacatat
    25681 accaccacca atacctcctg caatagccaa tactggaatc attcttctgt atcttaatat
    25741 aattccataa attataggtt ctgttactcc tgctaaaatt cctgtaactg ctgttgaacc
    25801 tgctaatacc tttaattctg aatttttttt agattttcta accttcataa ataaacctat
    25861 tgcaatacct atttgagcaa atactgctgc aacagctgca ccttctattg ggtctccacc
    25921 cattgtagcc aagttttgca aagtaattgg tgtgaagccc cagtgtagtc ctaatactgc
    25981 taagaatggc atagctatac ctattgcagc tcctgcaatt actctgttaa attcaaataa
    26041 tcccataaaa atattagata ttatatcacc aacattagtt gctattggtc caaataaaat
    26101 cacagttaaa ggaaccatga ctaataatga tatcatcggt actaagaata attgtaaatc
    26161 ctttaatata atcttcttta aaagcttatc aacatagtaa taaataaata tagcaacaaa
    26221 tatagggaat acaccaaaag aataatcaat tgctgttact ggcatcccaa aaaagttagc
    26281 accacctttt gctccaatta aagctgtaaa attaggatct aacaaagctg ctcctatcac
    26341 tccaccaaca tatggatttg cactaagttt tatagctaag gtaataccta agaatattgg
    26401 taaaaagtaa aatacagcat ttccagctgc tgataatata gcataagtgc tacttgtatc
    26461 agacatccat ttcatagtag ttagtattgt aagcagtgcc ttaatcattc ctgctccagc
    26521 tagtgcaggt attaatggag aaaatgcacc tgaaattgat tcaaatattt tagaaactat
    26581 tgaaactttt tctccctgtt cttcagtatt ttgtccttgt aaaactgctt tttttataat
    26641 agtttcaaag taatcagtta ctttatttcc aattaccact tggtattgtc ctccactatt
    26701 tactacagat agtacatcct tcatttttaa taactcttct ttgttagttt ttgaagtatc
    26761 tttcagctta aaacgtagcc tagttgcaca atgaactaaa ctaataatat tttcttttcc
    26821 tccgacatat tttataattt cttcagactc tctttcaaat ttcatacaat ctatcctcca
    26881 ttttcataat tttatgtata aaaatagacc taaatcaata caattagata ttaaaaacta
    26941 attataccaa cttaggtcat gcctgccaaa cagtaacaat cctacactat ttactttata
    27001 tttgataatc tatttaaatg caatgttaag tacaacaatt cattttcgct aattttccat
    27061 ttatacgtct tttcaagaaa gttttttact ttttttgcac aattgtaatc acttttatat
    27121 ttcattttta ttatatctaa caatgatgtg tctacatttg tattctcacc acttaattgc
    27181 ctttttataa aacatcttaa atgacttata aaccttgtgt agtcaaagga atattcattt
    27241 atttcgattc catagtgata ctttatgata tctaatacat tatcaattat cttagttaac
    27301 aataaagttt cttgcatatc ttttgtttct aactgagcat ttgcaaagtg tatcgtaata
    27361 aatgctattt cagaatcagg aatattttta tctaattttt ttcttaaata ttctacagct
    27421 ttcttactaa aattatattc attaggatat atttgtctta tttcccattg aagcgggctt
    27481 tgaaatacta atccttcatc cattcttttt aacatataac ttatatgttc tgataatgtg
    27541 attaatatac tatcattaag attatcaccc aattcttctc ttcccatttg aataatttca
    27601 cttacaagct caacatcttt taaaggtatc ctatctagta gtgaatgtaa ttcacttaat
    27661 gattcctcat tattgaatat aaatttcttt tcaattttat ctatttcaac taaatcttta
    27721 gtaaatttat taaatccaat tccttttccc attaatatta cttcttgtcc tgaattatct
    27781 ctggcaaata caacattatt attaaatact ttctttataa ccataaacta cctccactgt
    27841 aaacaaaaag agacctaaaa aagactaata atactttaag catttactag aatttttagg
    27901 tcacgcctga ttacatctat ctcagtaacg atccgtttat ttatctgtat gataacttta
    27961 ttttcattat aaaataataa ctgtacttag tcaatatata atttcatgta tatctctact
    28021 agattattgt cataagtatt cactcattct acttttttta tatgatttat ataatctaaa
    28081 catgctaaaa taagttttga agttatttca taacaaaatt aaggtcatat attttttatt
    28141 atatatactc gattatttat ccatatataa ggaaatcctt tattttgtaa ctacaataac
    28201 tgcctaagtt ttgattttct tttatctttt acttctcctt attaaaaaat aattctttta
    28261 ctgttgtatc taaggctcta aacagtttaa tcatcaaatc attgtttgga ttattaacct
    28321 tacctaactt actatatagc tttttaactg tatatcttta atagaccttt tggtatgcta
    28381 atccatttta taacactaat tcttgaatca agcatatatt acccttacaa agcatatctt
    28441 ataccctctg tagagttatg atagttgatg aacctgaaga aattaccctt acaagacata
    28501 tctgatacaa cctcacttat tagtttatat gtttcagaag tatataaata acacatactt
    28561 acttccaaat tcttaactgt tttcttagaa aattctaata tgatttcctt acatagcgat
    28621 tataacagtg tcttattaga tttcataaaa ttatacatcc tctttacatt taaactttaa
    28681 atttttatct attgtttatt aattaactgt atattcacta ttttttatca tttataatac
    28741 tcttcttatt atcttaaaac aattataatc taaccttaat cacatattat ataaatattt
    28801 ttaataaact taataattaa ataactatat ttcattatcc atcttataaa tgtttataaa
    28861 ctttccacta ttcttcttca ataaattcaa acaaattttc tactgtagta tcgaatactt
    28921 tagcgatatc catagctaac ttcaaagaag gattatattt cccattttct aagtgtacaa
    28981 tagtctcacg tcttacccca accaatttag caagttcatt ttgcgtcata aggagttttt
    29041 ctctgtattc tttaattttt gttttcatca atattgccac ttagttacat atcccccttt
    29101 tatcaaagta tatatacaac actaatttag ataaagactg tataaataaa actgcaatga
    29161 ggattatacc tgtatctagg tttgatatat ttatattact tgtataaggt gttgcaaaga
    29221 aaatagatat tattactata gaatatatca ttatttttat tgcaatctta ttaacctttg
    29281 atagtataaa ttcagatgat tcatcaacta aatcattata ttttttatta taaattgaat
    29341 aacatccaaa aactattaac caactcaacc ctggaagaga agcagtgctt ggacctttaa
    29401 aaatatattt agtagctaat acagctataa aaaaataagt tattacaagt aattccaaga
    29461 tgagctttga tcttacacca gtttctgatt tagacatata aaatcactcc ttaaaagtta
    29521 tatattctct atatttttta atttttgttt tcataaatat tattacttag ttacatattc
    29581 cctttttatc aaaatatata tacaaaatta attttgacaa agattgtatg aataaaactg
    29641 ctaagagcat tatacctata tccaaatttg atatgtccaa agctcttgta tgattaactg
    29701 aaaagagaac agctattaca aacatagagt ataacattat ttttgatgca atctgatcaa
    29761 cctttaacaa cataagtttt gatgcctcat caactaaatc attatatttt ttattataca
    29821 tgagattgca cacccaaaaa gctaataaaa ttataaaagt aaaccaaata ccttctcctt
    29881 taaaaataaa cttttgtaat aacaagtcta taaaaaaata ggatagcatc attaactctg
    29941 aaacaagatt tgccttaaca ccaagctctg atttagacat ataaaatcac cccttaaaag
    30001 ttatatattt ataacacctt atgttataaa tatataactt ttccaaataa attgcaatag
    30061 aatattgaaa atattcaata atatcttcat aaatatattt tataaaagat tttataataa
    30121 acattacaat acagtactaa attagtttat ttcatctatc atctcaatca tatcatttac
    30181 taactattat tctaatgagc atataacatt gaaatttata attatacaac ctaaatgcct
    30241 cccaatgtac cctcaaaaac atcatttcct gtaactgaag tataattggt atattttatc
    30301 actgaaataa aaaaacacta gtcaataaag actagtattt tcaagtattt tatcattctt
    30361 tttttaatta ttggagacga atcatcagaa taatgcccat aatttatcta tatgcaaatt
    30421 ctaaatttaa aataaagttt cagatgaatt tactattcta tgctatgata caacagaaaa
    30481 aactgaaatc tatgagtggt agcttcatat aatcctagtt cctacagtaa tcaaaactga
    30541 ggtttattct atatgtaact aatgataaat tttattatag gagttattag ctctatttgt
    30601 gttactataa gtagtaatta tatatatgat aattttttaa cacttttctg ttggctatat
    30661 agaaagtggt ttagaattta aatttaaata aaatttaaat tcaccaaaaa gaaatctata
    30721 ttagaaacca ctactctatt ccaccagatt gtaatttttt gttctttata tcacaatgct
    30781 ataccatatt aaattgaaaa ataaatttta aatttatttg ttacaattca ttaattcaaa
    30841 aaaatatttt gagtcatgag ttttgtaaag atttagccat gaatttaatg cttctgattc
    30901 aaaaacatct aaaagtttca atacttgaaa cgtaaattcc aacggagcga ttcctgaagc
    30961 cgtaactaaa ttttcatcac taactacaga ttccgtttta taatattttt ctccagagta
    31021 atttggtatt accattttaa gaaactctaa gtcattactt gtatgatttc taacatctaa
    31081 tagccctttt ttagctaggc ctaatgtagc tccacatatg gctgcaacta tagaatcttg
    31141 tattaaagct tctttaactt tttctaatat tatctcatga ttcatggata accaagtatt
    31201 tccccctggc aaaattaata aatccttact ttccatatta aactcatcaa ctgtaatttc
    31261 aggtaaaact tttaacccac ccattgttat aataggattt ttatcaattc ctacagtaat
    31321 tacttttagt ggctttaatc cttttttaaa atatcttcct gtatttaatt cagcagttaa
    31381 atatgccact tcccaatctg acatagtatc aaatacatac atatatactt ttctattttt
    31441 catctttttt actccttatg taattaattt atatatctaa ttataaaata acttaactga
    31501 catattcagt cagttaagct attaaatttg ataataatca attaattcta gtaaatcttc
    31561 aattattttc ttcctaaggc ttagtggttc aattacttgt attgtctttc tataaggtaa
    31621 aagtatattt ggtatgtact tgcatattac ttcctcttcg agcataaata ttattttatt
    31681 aggtgttctt tctttcaagt agtgccctaa aaaccaatgt tggcttaaac tatctaaaga
    31741 atctatactc ccttctacaa ctaattgaat tatattttgt tcatctttta tttcaggtat
    31801 aattcctttt ataaaaaatt cacttgctga aaattctgtc ggtctgttaa atgtatttga
    31861 tgtttctatg atttcactaa tcctatccac tctgaaacta cgtacctcat ttctcatatt
    31921 acaaaatgca actacgtacc aattattatt ccagtgaaca attccatatg gatttataat
    31981 tctatatttt gattttgctt tacgacctgc atgatagttg atttctacag atagctcctt
    32041 taatatacta ttctccaatt ttttaagtac tgattctata gacactttcc caattggttt
    32101 tattacttca agggctctta agtggtttgt taaaatatct tcttggtttt gatttgaata
    32161 catttttaac tttaatatcg atttatctaa tgcttcgtct aagaagtatc cttctttttt
    32221 agcaaataga gctgcatgaa gtagagattt ttgctcttct atatcaaaaa aaagaggtgc
    32281 tttagtaaaa ttatttaaaa ggctataccc accattatga cctgtatctg aaattatagg
    32341 tactccactc gcagaaagta catcaatata tcggtacact gttcttatat ttatttctaa
    32401 tttttcagct atctgttttg ctgtaatttt tttgtcagag tttaacatcc acagtataga
    32461 catcatattg tcaattttag ccatgtagtt tcacctcttt ctatttgtta tataaagtta
    32521 ttatataact ttttgattga ttttttacaa aaaaacatta gtaaactgct aataatttat
    32581 actaagattt atttcaaata tttatagata gtctttttct atagttaagt tatccattaa
    32641 attacatagt agtatatcca ttttagttga tatattttca gctacctccc ttgaatatta
    32701 ttacaataag tctaaatctt ctttcttcca taaatatatt atgaggaaag actatcatct
    32761 ttaatggttt aatagtataa aagatattaa tttaaaaaaa tctattttta tatcagatgg
    32821 gtattgtttt ttataaaact gaaaaaatca cctaatagaa tctatgattt tttaacataa
    32881 cctccataaa ttagtaccaa ttatcacttc aaattcaaat ttttatctga agaatataca
    32941 atcatatttc catactcaac ataatattga gtgtttggct tgtatttaca aattattatt
    33001 ctaacaaaaa caactataca tagattaaaa tcaccttcta aacataatta ttaaaattta
    33061 attaatgata tcaattccat taatctcttt taataaaaaa ttctcttttc tatatgaaaa
    33121 ttatttcaaa acttttaagt taaatacaaa aatgactata aatagatttt tatttacaca
    33181 aatctattta tagtcactat agtttatcca ataaaataat aaaataaata tgtacacaat
    33241 aaatgttatt tacgtcttaa gtatttattt gttagatttt taattaaaaa aaacaagcta
    33301 atgaacatta atgctaaatc tagcattata ttactttgat ctcctgtttg tacattttga
    33361 cctacattac tatctaaact ttcatctgta tctatattta tatctggttt tccattccca
    33421 tctgtatcta tatttatatc cggctttcca tccccatctg tatctatatt tatatccggt
    33481 tttccatctc catctatatc tatatttata tctggttttc catctccatc tatatctata
    33541 tttatatctg gttttccatc tccatctata tctatattta tatctggttt tccatctcca
    33601 tctgtatcta tatttatatc tggttttcca tctccatcta tatctatatt tatattcggt
    33661 tttccatccc catctgtatc tatatctggt ttgctatttc ctccattatc tgggtctggt
    33721 ttactatttc caccgttatc tggatccggt ttgctatttc ctccattatc tggatctggt
    33781 ttaccattgc caccgttatc tggatctggt ttgctatttc ctccgttatc tggatccggt
    33841 ttgctatttc ctccattacc tgaatccggt ttgctatttc ctccattacc tgaatctggt
    33901 ttgctatttc ctccattacc tgaatccggt ttgctatttc ctccattacc tgaatctggt
    33961 ttgctatttc ctccattatc tggatccggc ttgctatttc ctccattatc tggatctggc
    34021 ttatttagat ttggattacc tgtatcataa cctcctgagc cagtatctac atttttatca
    34081 ggcttccaat aatcatctac tccatcatta tctgtatcta ttgctggacg attataatct
    34141 gtatcagggt ttccatctcc attggtatct atatttttat caggtttcca aataccatct
    34201 ccatttccac cttctgtact aggcttccat tcacctgtat tatcagtatc tatgttaata
    34261 tctggcttcc aatcaccact cgtatctata ttgatatctg gccttccatc accatcagtg
    34321 tctatattta tatccggtat tccatcccca tctacatcta catttgtcat atctaacttt
    34381 aacttctttt catcctcact accttcaatg taatttatat catctccaat atattttaca
    34441 gtaatagttt gttcagtagt aaaaatatga tgattgctat ctaaaggtct ccattcaata
    34501 gatgcagtac tataattatg actctcacta cccttattat gagtttttaa ttctactgga
    34561 tctttatatt cctttccatt aatatagaat tgtacgtaac ctcttggaga ggcacaatct
    34621 ggtccttctt tcttatctgg tgaaatagta acatttaatg taaacgtatc tgttggttta
    34681 aaaacatcag ttttagcttt agactcttct acaactaatg aagtttctgt atcagcagga
    34741 ataatttctg ccttataata tgctctatgc ccccaataag cttcattaaa aggatataca
    34801 tttgaaaact gtgttgatgt aatgcttaat tgatattttc cagcatcaac ttgctttgta
    34861 aggtcatcac ttcctaaatt tgtatctggt tccattgttt cctcatttaa acgttgagaa
    34921 ctaatagtca taaatttttt atctgtgagt ttttcacctt taggagtaat tattggaata
    34981 cccccatctt ttaatatcca atctaaaaat aatttttcta catcttcttc aaatggtaat
    35041 ccatcatatc ttttaataat caaatatggt ggagtatcat caatagtaaa ttcttcatat
    35101 tgctttaaaa attgagaacc tgaacctata tctttaacat aaaaatcatc tggttttata
    35161 tttcctccag taattttatc tataacaact aaattaacag atacttcaga tcccttaaat
    35221 tgatcagaaa accataagta aagaacccct tgatcatctg tataactagg tgctccataa
    35281 ggataaattt ttccatcaat tagcatttta aaatcatcaa ctaatgtagt actaacttta
    35341 tcttgtccta aatactttaa agatatttta ttcataaaaa cttttgtttt cttttctaaa
    35401 tcaccgtatg caactgctcc acctaaactt ttgaatgttg ttgcttttaa tgaaccacct
    35461 gttactataa catctcctgt tgtagcactt aggtcaaaaa tccaatctcc cactcctgtc
    35521 atataaggta aaatcgttcc tccagttaca agtatacgat atcctctatt tgttcctgga
    35581 ttaacacatc catccccaaa tgcactacca tgtgcacctc catatgaata ggaaagtcca
    35641 ccattaattg taatatttcc actataacca gaacctgtgc taggatctac tactgaggga
    35701 cttaatgcat tctttgttgt tgtatagtat cctccaccta ttccagctcc atggtatgca
    35761 cctttagcag ttatagtacc accattgatt gtgatatctc caccactacc attaccgcct
    35821 ccacctattc cagctccata tcctgtatta cgactcgatt cttgacctat agcagttata
    35881 gcaccaccat taataataat atttcctcca ctttcagtat ttccaccgcc aatacctgca
    35941 gaataataat ttccaacaac ttttaaactt cctctattat tactatctag tagtgtaagg
    36001 cgatctcctt tttttaactt gtctccattt tctagagtta catctctagg aatttttcca
    36061 ccctcaggaa ctataaactc tccttcaata tctctattta caacagaatc atcaatagtt
    36121 agagtagtac cttttcctgc tctcatacct ggataatata catcacctga ctctagcttg
    36181 ttttcactac catcttgtaa tataacatgt gcatatgcac caccatcttt atctggtgtt
    36241 aaagtaaatg ggctattaga tgcagtttta atatttactc catcaaatat taattcagct
    36301 tttacatctt taggtataac aatcctatgt gttgttggag tatttggatt attattttga
    36361 attgtcattt gttttccact aagaatagtt aaaacgccac ttgcaaattt aaaatcgaca
    36421 tttatttctc caccactaat tttaaagtct cctacagatg taggatcttc tgcagtggct
    36481 tcttccacaa ctaatgcatt catatcttct tctgcatttc ctgcattaga atcttcttct
    36541 gcatttcctg cattagcatc ttcttctgca tttcctgcat tagcatctcc ttctgcattt
    36601 cctgcattag aatcttct
//
LOCUS       DBGJFU010000027        35380 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.027, whole genome shotgun sequence.
ACCESSION   DBGJFU010000027 DBGJFU010000000
VERSION     DBGJFU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.027"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            98..1162
                     /locus_tag="ACOIAS_001306"
     CDS             98..1162
                     /locus_tag="ACOIAS_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HGZ2701022.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1320..2006
                     /locus_tag="ACOIAS_001307"
     CDS             1320..2006
                     /locus_tag="ACOIAS_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2701023.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYKDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            1996..3027
                     /locus_tag="ACOIAS_001308"
     CDS             1996..3027
                     /locus_tag="ACOIAS_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2701024.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            3109..3774
                     /locus_tag="ACOIAS_001309"
     CDS             3109..3774
                     /locus_tag="ACOIAS_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701025.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            3787..6204
                     /locus_tag="ACOIAS_001310"
     CDS             3787..6204
                     /locus_tag="ACOIAS_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2701026.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSST
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(6385..7176)
                     /locus_tag="ACOIAS_001311"
     CDS             complement(6385..7176)
                     /locus_tag="ACOIAS_001311"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2701027.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(7209..7796)
                     /locus_tag="ACOIAS_001312"
     CDS             complement(7209..7796)
                     /locus_tag="ACOIAS_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2701028.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(7973..9364)
                     /locus_tag="ACOIAS_001313"
     CDS             complement(7973..9364)
                     /locus_tag="ACOIAS_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HGZ2701029.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(9554..10852)
                     /locus_tag="ACOIAS_001314"
     CDS             complement(9554..10852)
                     /locus_tag="ACOIAS_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HGZ2701030.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGYPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(11209..12084)
                     /locus_tag="ACOIAS_001315"
     CDS             complement(11209..12084)
                     /locus_tag="ACOIAS_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HGZ2701031.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(12277..12963)
                     /locus_tag="ACOIAS_001316"
     CDS             complement(12277..12963)
                     /locus_tag="ACOIAS_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGZ2701032.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(13012..13545)
                     /locus_tag="ACOIAS_001317"
     CDS             complement(13012..13545)
                     /locus_tag="ACOIAS_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HGZ2701033.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(13589..14221)
                     /locus_tag="ACOIAS_001318"
     CDS             complement(13589..14221)
                     /locus_tag="ACOIAS_001318"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HGZ2701034.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMGSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(14311..15390)
                     /locus_tag="ACOIAS_001319"
     CDS             complement(14311..15390)
                     /locus_tag="ACOIAS_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HGZ2701035.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(15523..16869)
                     /gene="pepV"
                     /locus_tag="ACOIAS_001320"
     CDS             complement(15523..16869)
                     /gene="pepV"
                     /locus_tag="ACOIAS_001320"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HGZ2701036.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(16938..18218)
                     /locus_tag="ACOIAS_001321"
     CDS             complement(16938..18218)
                     /locus_tag="ACOIAS_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2701037.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(18345..18605)
                     /gene="hfq"
                     /locus_tag="ACOIAS_001322"
     CDS             complement(18345..18605)
                     /gene="hfq"
                     /locus_tag="ACOIAS_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGZ2701038.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(18719..19654)
                     /gene="miaA"
                     /locus_tag="ACOIAS_001323"
     CDS             complement(18719..19654)
                     /gene="miaA"
                     /locus_tag="ACOIAS_001323"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGZ2701039.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(19692..21659)
                     /gene="mutL"
                     /locus_tag="ACOIAS_001324"
     CDS             complement(19692..21659)
                     /gene="mutL"
                     /locus_tag="ACOIAS_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGZ2701040.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(21670..24513)
                     /gene="mutS"
                     /locus_tag="ACOIAS_001325"
     CDS             complement(21670..24513)
                     /gene="mutS"
                     /locus_tag="ACOIAS_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGZ2701041.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(24674..25408)
                     /locus_tag="ACOIAS_001326"
     CDS             complement(24674..25408)
                     /locus_tag="ACOIAS_001326"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701042.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(25377..26390)
                     /locus_tag="ACOIAS_001327"
     CDS             complement(25377..26390)
                     /locus_tag="ACOIAS_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2701043.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(26412..27182)
                     /locus_tag="ACOIAS_001328"
     CDS             complement(26412..27182)
                     /locus_tag="ACOIAS_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701044.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(27157..27447)
                     /locus_tag="ACOIAS_001329"
     CDS             complement(27157..27447)
                     /locus_tag="ACOIAS_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HGZ2701045.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(27578..27677)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      28297..28386
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            28439..28615
                     /locus_tag="ACOIAS_001330"
     CDS             28439..28615
                     /locus_tag="ACOIAS_001330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701046.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(28730..29086)
                     /locus_tag="ACOIAS_001331"
     CDS             complement(28730..29086)
                     /locus_tag="ACOIAS_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2701047.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            29416..30255
                     /locus_tag="ACOIAS_001332"
     CDS             29416..30255
                     /locus_tag="ACOIAS_001332"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGZ2701048.1"
                     /translation="MTNADGLSESQMQKIARELNNSETAFIFNNLAKNHQENYDVHIR
                     FFTLTNEVPICGHATIAAHYVRAVENQFNNKTVFQKTSAGILPVDIIKKDNDYEIIMT
                     QGKIEVSPPLQQTYQKRLLNAFGILKEDIRTDCPIAISSTGHSKVMIGIKELSTLNSL
                     SPNLYELSKISQEIKCNGYYIFTLHNKETLQVHGRMFAPAIGISEDPVTGNANGPLGA
                     YLVHYKVIPTPNTEFIFEAVQGETIKRKGKMLVKVIILDNKPVEVKIIGKAVIAFSTN
                     IEI"
     gene            30486..31097
                     /locus_tag="ACOIAS_001333"
     CDS             30486..31097
                     /locus_tag="ACOIAS_001333"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGZ2701049.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            31395..31733
                     /locus_tag="ACOIAS_001334"
     CDS             31395..31733
                     /locus_tag="ACOIAS_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HGZ2701050.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(32140..33576)
                     /gene="miaB"
                     /locus_tag="ACOIAS_001335"
     CDS             complement(32140..33576)
                     /gene="miaB"
                     /locus_tag="ACOIAS_001335"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGZ2701051.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(33824..35284)
                     /locus_tag="ACOIAS_001336"
     CDS             complement(33824..35284)
                     /locus_tag="ACOIAS_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HGZ2701052.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSA
                     RLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVN
                     TVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTL
                     VNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLV
                     NDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGM
                     YKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYI
                     KIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLG
                     NAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT        12017 a         5557 c         4032 g        13774 t
ORIGIN      
        1 acgcttaaat tgaaaatata actttattct tatataatta aattatacaa caataaagtt
       61 atatttttta aatatatttt tactaggggg aatagaattg aaaagggttg atgagagaaa
      121 tactatgttt gcaagagcaa attacaaaaa agatagtact gcttataatg actattataa
      181 gaaaaatcct gacaaaaaag aaattgatga tagtataaga aatagaccaa atttatgtag
      241 tgaaggaaca atgacttaca atgagttaaa ctctccaatg gcaagtagtg cattcgattt
      301 tttatctgat atcaaatctt tatgcgaagg aaaagtttct gatactaaag tagatgtaga
      361 tgcaaagtca atgacaaaaa aaattaaggg tcttgcaaag cagtatggag cttctgtagt
      421 tggtataact aaattaaaag actatcatat ttatacaaat aggggtagac atgaagaaaa
      481 ttacggtgaa gaaattaatc taactcataa gtatgcaatt gtatttggat gtgagatgga
      541 taaagaaatg ataaatagag ctccaatgat atgtgaagtt atagaaacat caaagtgcta
      601 tgtggatgca tctatcgtcg gcatgatttt aagttactat ataagaaatt taggatatga
      661 tgctagaaat catatggatg ctaattattt agtaatgcct gtttttatag ctcgtgatgc
      721 tggtttagga gatataggaa gaaatgctat acttacaaat aaagattatg gctctagact
      781 tagattaggt gttgttacta cagatattcc tcttttagaa gatgaatatg ttgattttgg
      841 actagaagat ttttgtaagg tctgtaaaaa atgctctttt aactgccctt ctcattcgct
      901 ttctaatgat attaaaattg gtgatgatgg aaaatataat tgggtaattg aacatgagac
      961 atgctatatt aaatggagat atttaggtac agattgtggt atgtgtatat ctgcttgccc
     1021 atttagccaa aatctcgaaa ctataaagaa tactacttca tttaagggta ataatgagct
     1081 tattcaaaaa gctttagatg agtatacttc aaaatttggt aaaagaatat ttatacctgg
     1141 aaatccatct tggttgaaat aattttattt aataaagaat ctgtactaga atttatcata
     1201 ggacaggttc tttattaaat atataaattt ttaaatactt tttctcgttt ataacaattc
     1261 agcaatgttt ttagtctata attgacatta taaaatatta aactaaaagg agtaactgta
     1321 tggaaaaaat attagtagta gaagatgatt ctatattaaa taaaactcta tcatataacc
     1381 taattgaaga tggatatata attacttcaa aatttacagc taaatctgca ctaaagtcta
     1441 tttttgaatg tgaatttgat ttaataattt tggatattaa tttaccagat aaaagtgggt
     1501 ttgaattatg taatgaaata aaggaaaatt acaatatacc aataattttt ttaactgcta
     1561 atgatatgga atgtgatatg attaaaggct atgaacttgg agctttggat tatataacaa
     1621 aaccatttaa tatcaatata tttaaacaaa aaattaaagc ttttttaaat catcttacaa
     1681 taaaaacaaa acaagattac tataaggatg gatatcttga aattaacttt tctgagttat
     1741 ctgccaatat aaatggaaat caaattatat ttacaccatt agaatatcgt actttaaaac
     1801 tgcttactga aaatcctaaa agtattttaa caaggaaagt acttttagaa aaactctggg
     1861 atatagatgc taactttgta gatgaacata ctttaacatc tgttataagt aggattagaa
     1921 gcaaaattga aaaagacaat cttaagtata tcaaaacggt atatggtatg ggatatatgt
     1981 ggttaggtga aagaaatgaa ttttaaaaaa ttttctttta gttctattta tttaataatt
     2041 gctgttggta taatttttac agctatttta tttagtatct caatttatct aataactggt
     2101 gatttaaaac taattgctct attattaata tatacaatgg tactttgtgg atttatagca
     2161 ttaataatat ttttacttca taaaaaaata gttatatttt gttctaaaat atgtcacact
     2221 ttagatagta tgatgaatag tgaagataat attgaaattg aattagaaga agaaactatt
     2281 ttatcacgta tcaatcacag attaattaga atgtatgaag ttattcaaga aaatcgcaat
     2341 agtattgcta tagagaaagc tgatttacaa gaactagttt ctgatatatc tcatcaaatt
     2401 aaaactccta ttgcaaattt aaaaatgatt aatactactc tactaaatca aacatttaaa
     2461 acagaaattc aaaaggattt tttaatggat atggaaaacc aattagataa attagatttt
     2521 ttaatgcaat ctatggtaaa aacctccaga ctagagacag gtataatcac actatctaaa
     2581 aagaaaaatt caatttatga aacaattgct acagcactta gtggaatttt gtttgatgct
     2641 gaaaaaaaga atattgaagt tacagttgat tgtgacccaa atttatatat aattcatgac
     2701 aagaaatgga cttctgaagc tatttttaat atattagata atgctgtaaa atacacaagt
     2761 tctaatggta agatacgtgt agctacagaa tgttgggtta tgtatacaaa aatagatatt
     2821 attgataatg gaaaaggaat atcagaaagt cgccaagcag aaatctttaa aagattttat
     2881 cgtgaagaag atgtccatga catagaaggt attggaatag gtctctatct ttcaagaaaa
     2941 attatatctc ttcaaggtgg ctatattaaa gttacatcag atattggaaa agggtcgact
     3001 ttttctattt tcttacctaa taaataaatt tttaaaaatt tcatttgtaa tgattctgca
     3061 acattttggt tttatactgt atttacaaga tgttaaggag gtattattat gagtatttta
     3121 caagtaatta atttaaagaa atattatgga caaagttcaa acattacaaa agctcttgat
     3181 ggtgtaactc tatctattga aaaaggcgaa tttatttcta ttgtaggtac atctggtagt
     3241 ggcaaatcca cattattaaa tatgatggga ggattagata atcctacatc tggaaaggtt
     3301 attataaagg gtaaggaatt atctactatg tctgatgaac agcttacaat ctttagacgc
     3361 agaaatatag gttttatatt tcaaaattat aatcttgttc ctttacttaa tgtatatgaa
     3421 aatattatct taccaatagg attggatgga gagaccatag ataaggattt tataaatgaa
     3481 attataaata tgctttctct tgaagaaaaa ttatatgata taccaagtaa tctttctggt
     3541 ggtcaacagc aacgtgttgc tatagctaga gcacttgtaa caaaaccagc tattatacta
     3601 gctgatgaac caacaggtaa ccttgatagt agaactagtt cagatgtact gagtttactt
     3661 aaagtgacaa gtaaaaaatt caaccaaaca attgtaatga ttacacacaa taatgaaatt
     3721 gcacaactaa ctgatagaat tattcgtatt gaagatggaa aaattgttaa ctaggaggtg
     3781 tagtatatgt ttagaaataa taatcgcaca tttataagaa aaatagcttt aaatgattta
     3841 aaaattaata aacttaaaac atatctttca ggtatcatta taatgatttc tacatgttta
     3901 ttattaacag tgaccttagt ttcatataat gcctctgtag atttagttaa tgcatctcca
     3961 tatcatgcta tatataaatc tgtagatgaa aaagcaaaaa atatactata taaaaataaa
     4021 aactttagtg gcgtaggaac ttacaaatta attggaagta ataaaaagac agattgtatt
     4081 atgtcgattg tttatgcaga tgatacagca attaaactta tgaattttca accattgaaa
     4141 ggcaaactac ctacaaataa aaatgaaatt gctatttcag aaaaatattt acaggaattt
     4201 ggtttagata aagatattgg agatagcatt aaattaaatt attatgatga tattacgaat
     4261 aaagaagttc aatgtgattt tattattgtt ggatttctag aaaattatta tcaagataat
     4321 gccaagcaat actatacagt cgtttcaaat gattatttta agaatatagc ttctacacct
     4381 ttacaaaata aaagtaatac ttttgacgag tcaagacctg atactgtaga tgtattagtt
     4441 aaactatcag aagaaaatac agagaaagat tcagcttcaa ttaagacaca gttaaataaa
     4501 attggtttat ctttaggtat taagaaatat aatatatact taaatgataa ctatattgaa
     4561 tcaaatttaa ttgatggtga gcagattatt acagttttag tagttggaat agttgttctt
     4621 ttttctagcg tttttgttat atatagtatt ttttatattt cagttgttaa ttcagttcag
     4681 atgtatgcta agttgaaatc gttaggtatg acttcttttc agttaaaaaa aattatttct
     4741 ctacaaggaa atattttgtc aattattttt attccattag gggttatagc ttcttgtata
     4801 attgcttaca taattcaacc attagcatgg caaatgatag ctgatttatt tataatttta
     4861 attttatcac tggtaatgtt tttaactgtt agaatttctt tatttaaacc agctagaatt
     4921 atttctaaaa tatctgctat tgaagccatg caatatacag aaactaaatt tagaaaaaaa
     4981 gcaaaaaatt ttagttatat aaatataaaa aatctagcat taaagaatat cgagtcaaat
     5041 agaaagaaaa atttgattgc attaatatcg ctaagcatta gtggagtttt atttatttca
     5101 atcgctaatc ttgctaattc aatagatttc aaaaaacaac tatctcaaca atttgtctat
     5161 aacgaagatt atataattag cattaagagt gataacttat atgagcatat tacagaaatc
     5221 caacaaaata atccactaac agattctctt aagaatgaaa ttaaatctat tcatggagta
     5281 aaaaaattaa ttgaatccaa atcaattaaa agtaatatta tagaacctta catcaaagat
     5341 agagacgaaa aacaatttat tactctaatt aaaggtatta cgcctgaact ttcaaaatca
     5401 cttgaagaat atattgtcag tggaaaaata gactatacac atttagattc agatagttta
     5461 attataaata aatatagaac aaaatattat ggattaaatt taaaagttgg agatacagtt
     5521 acatttaata tctttagtgg aaatgatatt attaaaaaga aaatgaaaat tataggaatc
     5581 attgacaatt ccaatacagg aggtatgttt tttgcttcag acaaaacttt agaatctcta
     5641 acaccatata atacaaattt agatttttct attttaatag ataaaaagca ttctaaagaa
     5701 gttgaagaaa agttgttaag tataattcag aaaaattcaa atttaatgct ttattcatat
     5761 gaagatgatt atagaatgat tactagagca tttcaatata taattatagc ttcatacatt
     5821 tttgttggtt taatttcttg ctttggtata ctaaatatga ccaataccct aataaacagc
     5881 gttttaatta gaaaaaaaga gtttgcatta cttcaagccg ttggaatgac aagaaagcaa
     5941 ctgcgaaata tgctttatag agaaggtcta aacataagta tcaaagctat atgtacttca
     6001 agtacactag gttattttgg ttcaaatcta ctttgcactt ttataaaaga tgtaattaga
     6061 ttagacttta ttaattttaa attttctata tttactattt taattttttc attcgtatta
     6121 attggtatac aagttttagt gactgaatta cttgtaagaa atatagaaaa aaaatcactt
     6181 acagaacgct tacgttctca gtaagaatgt atataaaaaa tagccatttt ttatacaagt
     6241 tttaatatga ttttttaaaa gctataggga tagaaaattt tgactttaca taattttcta
     6301 tccctatact tgtttttatt ttatacagat acacttactt atatctccga gcaacatcac
     6361 cagagacata aacaagcata ttccttattt tctaaatatg tcaattgcat gacttgtaac
     6421 ccctactaaa tcttcacgtt cacaagtagc aaggtgatat tttaaatata atgcaaatgc
     6481 atcatcatcc atagagtcta ctgcttcacg catatataaa gccaaaccat ctgatgcgac
     6541 ataatgcagt ctagttacat taaatgcaga catcaaatca tcaatgtttt ctttacgtac
     6601 aagctcaaac aaatcctttg gttcagactt agcagcaaat gtctgtggat caatcaaacc
     6661 tttttcaata tattcagata cattaatatt tcctctatgg aacccctcgt caatgagaca
     6721 gccatcagat ataacatatg cagcaaaaac cacaccacca ggttttgtta cacgaattgc
     6781 ctcatgtaaa gcttgttgtt tatcttcttt gttataaaga tgatatagtg gtcctaacag
     6841 taacgtaata tcatatttat tatctgggaa aacagataaa tccatagcat taccctgagt
     6901 aatgcttata ttttcatttg attgagtatt tttatggaaa acctctatat tatgttcgac
     6961 taattccact gcgtcaacat catatccctg acgtgccagt gtgtgagaat aacgaccagt
     7021 tgcagcgcca atttcaagca cacaatcacc tgattttatg tacttatcaa tgtaatgcat
     7081 agtagtgaga aattccacca ttccatgttt taatgcaagt cgactatctt catcataagt
     7141 attataaaag tcaattaagt attgttttgt ttccataatt tcacctcttc tatattagtt
     7201 aataaacatc aaatagacaa attccaatct ggtttttctt gtcttataaa agctgttaat
     7261 atatttttca acattttttc agcactatat ctatctaatg ttaatttttt tgttgctgtt
     7321 aagacagcta tgccatgagt ataaagaaat aaatctaaaa atattacttt tgcacattcc
     7381 aactctattc caatgctttc cgaaaaaagt cttgcctttt tttcattgtc aatttcttta
     7441 taaaaatcat ttgcctcttc catcatcaaa tctaaatcat ttataaataa caacttaaat
     7501 aaatgagttt cttcttgagc aaattcaatg tatgaaagcg gaagcagtaa ataaggactt
     7561 acacttttag aattactata attaacaaca tactgattat agtattcata tgcaaaagca
     7621 agaaattcat tttttaattc attcatgttt tcgtaacaag taaaaattgg tcgagtggaa
     7681 cactgtaatt tacttgcaat actcctcgca tttacagctt caaatcccac ttctcttgta
     7741 atatccaaga ctgtattcaa aatcatttct tttgttatct ttactttagg tggcatatat
     7801 gttcacctct ccattaaatt tttataaaac acatatgtta tataactata gtaacatatc
     7861 aagaaattat gtcaagaaaa agctttgcat ttttttgcaa ttcctaaaac ttataaataa
     7921 aacatgagtt gcaattaatc tattaataga taacagcaac tcagattttc ctttaaatac
     7981 tcttcttttt gtctaatatt tcatatacac aatcttctat tgaattaata tctttttctt
     8041 ttcttttttt agaatcaatt aaaatgaaat acagtataaa acctacagca agtccccatg
     8101 ttccgccttt agcagcaatt acacctccca taagtccaat caatcccttc tcaacacctg
     8161 tattacatag ctccattgat agctttgtac ataaatatcc ttgaactaaa agtgttactg
     8221 acaatgctaa tggtataaga ggtgtagcaa tctgcgcaat tggataaaaa cagataaaaa
     8281 ttgctgaaac ccatactaat gtacccatgc cactaaaaac agactgcatg gcttcctttc
     8341 caaccttgta cctttgagca acacttcctg taagtgtcgc agctaatgga ccgcacattg
     8401 gtatatatgg agaaaagatt gccataaaaa tatttcttat cccacttata acattagacc
     8461 tatttgaatt gaaatctata tattcatctt ttctattttc actttcagaa agtactaagt
     8521 tttcacctgt tataaaatca ccaaaggcaa ttatgtatat cataaaagcc attggtatcc
     8581 cttgtataaa taacattaca cttggaaaac ctattccaaa aggagatact gtatttatca
     8641 gatttttaaa atctactatg taaactaaat tgtcaaaact aaatctagga atttctattt
     8701 ctttactaaa tatacctact attaaactca ctaaaatagc tgatactaca ccatattctc
     8761 ctaaatgaaa taaaaattta ttcttaactt ttagagtatc aaatctttca gaaaacatta
     8821 caaaacaagc aaccaaaatt cctactgtta tagatatagg ataagaatca aatctccctt
     8881 taggattaaa ctctgatatt atagtcgata taccgacacc cattagtatt ccacctttaa
     8941 tagaatttgg tatcttacta actaactttc cagctacacc agttataccc attaaaataa
     9001 atattattcc taaatctatt tgtaaagctg ttaatgcttg tattctttca atgcccatgc
     9061 tgaactttaa caaatatgca gttgtaagag ttatagaagc tgtaatccag ccaggtacaa
     9121 ctgggtctcc cataaaaaca tgcaaacagt ataatattga gttaatgata gccatactcc
     9181 atgccacttc atagggtaat cccaaaattt gtgtactaag gccaattgtg cctaaacttg
     9241 tcatacttga tattatacct tgaatagctt ctggtttctc aaatctgtaa tgtataaatg
     9301 gaattcttat cttaaacatt ccaaatttta tatgaggttg ttcccttcta gcgagtcttt
     9361 ccataacttc actccacctt tcaataactc ttataagttt acaactttat aattgtttat
     9421 aaattatcaa tgtcattaaa attagtatat aacatgcaaa ttatataaac aataaattaa
     9481 atctataaaa ttgatagatt ttgtgcatat atagaaagac cgcttcattt ttagttactt
     9541 aaatcactta atattagtta tttaaatcac ttgataaagg ttttttataa aattcaatag
     9601 ttctattcca tgattcaaac tgtccttttg aatttccttc tacagttcca cctgaagaaa
     9661 aaatccctct actcatactc atacataaag tctcctttag aggcataata tatggaattg
     9721 gtaacggata acccatatta ttgtatgtta ataattctac gttatatgga taattatttt
     9781 cttcaaagat ttctaatgcc tctaaacaag cttcataact attccatatg ttatcttctt
     9841 ttccagcaat cataagtata tctgctttgg aattttctat ttttattcta gcctcttttc
     9901 tattctctgc tcttttaaga cttttcttat aagtactatt aaatccaaat ggaatatctt
     9961 ttgatatatt ttttttctgt tcttcaaaga atatatcatt gtctactttg atatatggaa
    10021 atggctctcc tttatatgac catgatgaag tatcatttcc actcatcaat ccattaagag
    10081 cttggaaaca ataaatgtga ggttgtgata ctacaacctt tttaatttgt tcatatcttg
    10141 aagcaagtaa aaaggccaat tctccaccct ttgatgtccc atgtacaaat atttctttag
    10201 tactggtaat ttcattttca ctaatccact caaatacttt ttcaaaatat tctaatggaa
    10261 cctcttctaa tttatctggt aacccttcta aagcaaagta tgcaactgtt agtacattga
    10321 atcctctaga tgcaagtgga gctgctaaaa ggtctagagc gtctaaattt ccatcagaac
    10381 cacctagcat taaaattgtt ttattattag aattttgttt ataaaataat tttcctataa
    10441 actcatcaga tatgtatttt gtggtaatat cttctgattt aaatattctc tttaattcaa
    10501 cttcttttct ttcaatagaa gtttcaaata tcatattcat aattattggt ttatcaatag
    10561 atatattttc tgcaaaattt ttaccttcag ttttagattt ttccagcgaa taaattaacc
    10621 ccatacaatt atcagcctta taagtacctt ctataggttc aactaaatct aaatctactt
    10681 ctcctttttc atttgaacaa aacactccat aagatgaaaa ttcttctccc ttacaccatg
    10741 gaaattccat tttcacactt attttaagtt tagtatttgg aattaagtct gaaaccataa
    10801 tcttaagtct ttcatccaca aatgattcta cattgtatac atttactttc ataatctatt
    10861 tctccttata ataattattt atgttttata taaaacagct ttatctaata caagataaat
    10921 aaatttttaa attaagacac catattgact atttcatttt atcaacatat ttcatctttg
    10981 tcaacatatt gtctttttta gtaatttaaa attatggtct atatgaaatc cagttgattt
    11041 ttggctaatt aaagatagga atgctattag gtttagaata tatatattca aaataaaaaa
    11101 ccactcattt aaagaatggt ttttaatata aactatttgt tattaataag gatatttttt
    11161 tatttaaatt tatataatcg aacatctaat atttaatgtt taataaaatt atgcatttgc
    11221 aaaagtatat atggcattaa ctcttgctac atataaattc tcatcactac atccaatata
    11281 tctagcatat ttttcaatct tttcttttgc attttcaaca tcattttcat cgctctccat
    11341 taactcattt atataaatat ccattaatga cgctttatat tctcttatat catagcctaa
    11401 aaattcagtc aatctctcaa tttccttact aaaatctcca tcttctttta ccaaaaattc
    11461 gtatacatct tccctatatg gatttaaagt tattatttgt tttactactt tgcattcgtc
    11521 agccttccca ataaaaccat ctttataatt attaaataag gcattagatt tttttataga
    11581 ttcttcatca atataagaat gtaccgcagt tatacgattt tctataaggg catctacaac
    11641 agcataatga attttaaaac aatcattgta taaataattg ctcattttct ttattgtcga
    11701 ttctatttct aagaagctcg cgtggccatt ttttttattt ttatttggta taataaaagt
    11761 tttattcaaa ttattaaaaa gtctcttaaa atctaaatat ttttcacaat aaacactttt
    11821 aaaggtatcg atatctatag ttataattcc aaaacttact atagtctcaa ttcctttctt
    11881 aaaagactct tctatatatt tttctcctaa acttaaacct ctatcagata tttgctttag
    11941 attttgaaaa ctatttaaac aatctctttc aaagttttta gatatttcta aagcatataa
    12001 tttaaagatt ttccttatct tattataatt attttttgta tcatcaaatc tgataatagc
    12061 tccatttaca tcaaaagtaa tcattgagtt ttaacctccc cattagtttc atcaataaaa
    12121 taaatttaat ataaacttta taatcataat attaaactta tttatactat aattattaat
    12181 ttgatgataa aagctatttt ccttctaaat aaaaaatttg tctaaagttt ctaaaaaatt
    12241 tagacaaatc ttaggttatt actctttaaa ttatttttaa attattccaa atacgccaaa
    12301 taatattggt ataaatatag ctggtaacaa attggctact tttatattcg ttaatttaag
    12361 tacatttaga ccaagtccta taattaataa acttccagct gctgtcattt ctgtgactac
    12421 aggatcactt aaaaatccat ttaaaaatga tgctcccaca catataatac cttcatacaa
    12481 aaatacagat attgcagaaa acattacacc tatacctaaa gaagcagtaa atataattga
    12541 agctattcca tctataacag attttacaaa taaagtatca tgacttcctg taagaccact
    12601 ttctaatgaa ccaactactg ccatggctcc tatacagaat aataaactag atgatataaa
    12661 accctctgct atagaatctt ttttatttcc tttagaaaat ttactttgta aaaaagcacc
    12721 caacttattt acccatttgt caatatctat taattctcct atcagagcac ctacagctac
    12781 ggatattatt gtcactaatg tgttttttcc ttctagtgct cctgatatac caatgtacaa
    12841 tacacataaa gcaattccat tcattatagt atcgcttact ctttgaggta atcctccttt
    12901 tattataaga cctactatac atccacctgc aattgctaga ctatttgcta ttactccaaa
    12961 cattttttct cccctcgatt agctaattga aatttatcct attattaaaa cctaaacatc
    13021 tatactttgt attggatatt ttaaatattc caaaaattct tcaaaacctt ttctagatat
    13081 atttacagtt ttagtattta tgttaggatg aaaatttaag taatcttctt taattacatc
    13141 tttatctatg taaagctcaa cttttctttc tgtatcattt attaatgaaa aaggatttac
    13201 acttccagga agaagtttaa gtacattgta tagtctttca ggtgaagcaa acgataatct
    13261 agaacttcct atcttatctt taagtgagtt taaatcaact tgtttttctc ctttaactac
    13321 aataagataa tacttatttc ctttggcatt tcttaaaaat aaatttttac attgacaccc
    13381 ctttgttatt ccatctaaaa tgactaattg ctctgctgta tatacagctt catgctctac
    13441 tacatcatat tcaacattta atttatccaa aatatcatat attttagttt cattcatgct
    13501 agattcattt tcatcttgtt cttttttatt atttaaatca ctcatacttt tctccttaaa
    13561 cacttcatca taatatcatt gtgaggtatt attctactat agctattgct cttactggag
    13621 ctccatcagc atcattatac ttaaatggtg ctgctataaa tagaaatttt tctggaacat
    13681 tttctaaatt tgtaagattt tctatgatta tttttccttt ttcaaataaa atatgatgca
    13741 cttcaaatac tgatgtgtca tatctatcca ctgatagcat atctattcct atgcctttta
    13801 tattagtatt agctatatag tttgcagctt cttttgttaa tgtaggataa cctacatagt
    13861 actgcttttt atcccaaaat tttgaccaac ctgatttaaa tattataaaa tcactatttt
    13921 ttatttcatc ttcatatttt tttagatatt ctaattctat attctcattt cccttttcaa
    13981 gttccaatac ataagctttt ccaacaaaat tttctatatc caacttatct agggtctcac
    14041 ctttagtata catatgtctt ggtgaaccca tatgagttcc attgtgagaa aatacactaa
    14101 ttaatgtttc ttgataacca ttttcttcaa taatagctac tttcttgata tctggtctat
    14161 taggctctgc atatacaggc atgtcattat gtgtcacatg agttaaatca aaaaccttca
    14221 tctttatata ccccctatgt ttttattttc tattatatac ctaaattcat tttatttcta
    14281 tcagatttta ttccattata taccatacta ctatatattt tttataaacc tcaagggatt
    14341 taccgaaaaa tcttggaaag aattatcagt aaatataaca tcttcaccca aaaaacgttt
    14401 ataccttcct tcttctttct ttaaagtaat tctctgtttt tgtgaatgat tattaaaaac
    14461 cattatctta agaggaggtt tttttctatt agtctgcact ctttttaaca tgtattttat
    14521 gtgcatatca gcttcaggaa atgaatatcc acaaaatatt aaaaggtcag tatctcttaa
    14581 agttttttct gtttcattcc aaacattact taaaaatatg tttgacatat tcttaaaata
    14641 tgttggtggc actataatag gttctgttaa ctcaccacac tctaaacatt ttgtctctga
    14701 actattttca ataagcttca tgacaccacc ttcatgtggt gtaagagtta cactattaca
    14761 aatggggcaa taaagccaat tcaatgaccc atgtatctta tataatttga ttaatggttc
    14821 ttttggtttt ctccaattta actcctcttt aaaatttgta aactccacac cataatcaag
    14881 cataattggc aaatcattct tatctcttaa tccagtaact gtattgtcta tatgtatatc
    14941 ataatttgca ctaataaaag tagtatcaag aagcaaatca ttttccaaaa gattttcaac
    15001 taaaagttta tgatatttat tatctgttct aggtgtatta tgaattgctt cagccattag
    15061 cagtattaaa tattgtctta aaaaacgtat actatcactt ctgttattta gattttctag
    15121 tccaaaattt ctaaatgctt cttttctttg ttctgctaag tctaaaagac ctaaaacttc
    15181 ttcaaaggta ggaaaatttg cattatctat attatctttt actatatcaa tattgaacat
    15241 ctgtttaaag aaaatataca attctctatt cattttggta ttataatttg tgggatttag
    15301 ttttttaaaa tattcgctaa aaagttcatt ttgaataggt agattctctg ctgctgaagc
    15361 tcctgctcct aaaaatattg ctattttcat atagttcctc ctaatataaa tattactcct
    15421 atttttatat ccattaaata tccatatcat ttattttatg atacaacaat aaagctgtct
    15481 tttactagaa aaaatcctat tacaagacag ccttatatta tattaaatct ttaataattc
    15541 gtaaatagaa agagcatata tttgagtagc ttttataata tcctctacta ttaagtattc
    15601 attagcttgg tgttctgttt ctggttttcc tggaaaaata gctccaaatg caacacaatt
    15661 atctaaagct cttgcgtatg ttgctcctcc agatgaaagt ggtgttccat caagaccagt
    15721 ttcttcttca tatacttttc taagtgtctt aacaagaaga gtatcttctg gaacatagat
    15781 tgaatccaag aaatcatatt cttcataatt taaattgtat ttatctgtca ttttctttaa
    15841 ctcttttaca aaatcatcct ttttgtatgt tactggaatc cttacatcta ttcctgcaaa
    15901 ttctttttcg ttatcaatag ttactctacc tatatttact gttagtttac ctgatacatc
    15961 atccttgcaa ttaggaatta tattgtttcc attagcatct tcaccaataa cttctgctaa
    16021 aaacttaatt atattagaat ctatacctat attattaaga gctatacaaa gtctagccac
    16081 tgcatttata ccagtatcac ttgctgctga atggacactt tttccaatta tacagatttt
    16141 attaccttca acagtatact caaaatttaa tttatcaagt tcttttttta atttatcaga
    16201 gtattttcca atatagattg ctttacctgg aactgcattt aaagcctttc caactgacaa
    16261 ctctatatca cttttgctat cacaagtcaa atgaacttgt aaaagacctt tctctgcatt
    16321 tgttattggg aatctagaat ctggagtaaa tccatagttt ggtatttctt cattattttc
    16381 tttatattta tttatacatc tccaaagatt ttcttcatca gccccaaaaa tgaatcttat
    16441 cctcttatta aagtctacat ttaaatccat aagagctttc actgcataaa ttgctgatat
    16501 tgtaggtcct ttatcatctt gtgttcctct tccatacaat ttaccatctt ctaaaacagc
    16561 ttcaaatgga gggtaattcc agctttctaa atcaccttct ggaaccacat caacgtgacc
    16621 aagtatacct atcatttttt caccttgtcc tatttctgca tatccataat atccatcttt
    16681 gtatactgtc ttaaatccta aagatttaca taagtctaat atacctctta aagctttatc
    16741 tacattttca ccaaatgggc aattttcagt tgcttcagat ataacactag gtatttttac
    16801 actttcttgt attgaagaaa tcatttcatc ttgtagagaa tttacttttt ctttaatttg
    16861 ctctttcata ttatcaccct tcaattttca tcataatata ctaataaata ttttgacctt
    16921 ttaaaaaaaa aatatgctta ttttttcagt ttttttataa attcaatcgc ttttaaagtc
    16981 cccatttttg aatggtcaaa tgtaagtcca ccttgaaaat aaacattata aggaggtctt
    17041 attggagcat ctgcacttaa ttctatagaa gaaccttgta taaatgctcc tgctgccatt
    17101 atgacttcac tttcgtatcc aggcatgtcc caaggaacag gttttacata agaatcgact
    17161 ggtgcagctt cttgtatacc ttcacaaaaa cttattactt catcagcatt attaagtcta
    17221 atacattgaa taatatcact tcttaagtca tcatattttg gtaatacatc atatcctaat
    17281 ttttcaaaag ctcttgagca aaagatagct cccattacag cctgagatac tatataaggc
    17341 gccaagaaaa aaccctgaag tacatttcta gttgttccaa aagtaagacc acattctttt
    17401 cctattccag gagaagtcat tctataagat ataagctcaa tcaaatcttt tctaccagct
    17461 atatatccac ctgtcaatgc aagcccacca ccaggatttt ttattaaaga gccagccata
    17521 acatctgctc caacatcagt aggctctttg gtatctaaaa actcaccata acaattatca
    17581 accattacta tagcttctgg tttaacagac tttattactt ctatagcttc ttttatatca
    17641 ctaattgaaa gtgattttct ccaagaatat ccttttgacc tttgtatcat aacgagttta
    17701 gttctatcat ttattttgtt tttaattcct tctaaatcta agtttccatc ctctaaaaaa
    17761 tctacatcat catatgtaac accatactct ttcaatgaac ctttttcttc tcttatgcct
    17821 ataactccct ctaaagtatc atatggtcta ccagtaacag ataaaatttc atctcctggt
    17881 ctaactatac cttgtataca taaagtaagt gcatgtgtac catttacaat tattggtctt
    17941 acaagagcat cttctgtatt aaatacttta gaataaattt cctctatttt ttcacgacca
    18001 atatcattat atccatatcc tgtagtccag ttaaagtgca tatcacttag tttagattct
    18061 tgcatagctt ttaaaacttt atattgatta tattctctta tttcttttat ttcttcaaac
    18121 ttatctttta tttcttccat tatttcttgc gatagtttaa aagtatcatc atctattcca
    18181 tagtagtctt ttaataattc ttttgtctca ttaagcattt tatcacctgt ctctttatta
    18241 ttttatttaa acatgtaaag gagactattt tactaaaaca aataaattat agtttataag
    18301 ttagtataag tctcctctca atcatcttaa attaaattaa ttatctatct gttgttatta
    18361 ttattgttgt tttggttttg atttgttaga ttaatatact ttccaggttg tatagttgat
    18421 acagcatgtt tataaatcat gttttgttgt ctattatccc cttctattaa tattatataa
    18481 ctatcaaacc cctttacaag ccctttaact tgcactccat taactaaata tatagtaaca
    18541 ggtatccttt ctttccttgc attatttaaa aacaaatctt gtaaatttaa aactgtattt
    18601 ttcattagat ttgtaccctc ccaagattaa attgaacttc taaaatattc ctaccttata
    18661 ttctatataa gttatgacaa attttcaata ttaagttgta tacttaaaaa tttatatatt
    18721 attttataga atctttaata tataagatta tttcattttt taattcgtct atattttcat
    18781 attggtctaa atcaaaccat tttgctgttt tatatctttt aaaccatgtt atctgacgtt
    18841 ttgcatacct tctagaatct cttttaatta tttcaatagc ttcttcgtat gtatattctc
    18901 catccagata ctttaatatc tctttataac ctataccttg cattgatatc atatcttttt
    18961 taaatcccat acttaaaagt ttttttgcct cttcaaccaa tccattttta atcataatat
    19021 caactctcat atttattctt tgatacaaat gttctctatc tctatttaga actattatta
    19081 ttggttgata ttcttcatta aattttaaat cacttgaaaa atcattcatt ttcttaccac
    19141 ttagacaaac ttcaagagct cttatgaccc ttttagtatt gtttttatgg attctattag
    19201 cagattcttc atcaagttct tttaacttat tatgcatgta atctattccc ttttcagcaa
    19261 gctgtttttg aagttcttct cttaattcat tattggcatc agattgtgca aaatccatat
    19321 tatatattaa agagtttaaa taaagtcctg tgcctccagt tactaaaaca catttttcct
    19381 tttcatttat ttcatgtata tatttttttg ctctttgttg aaactctgaa actgaaaatg
    19441 gatagtcagg cttaacttca tctataaggt aatgcttcac tccttgcatc tcttcctctg
    19501 taacttttgc acttccaata tccatatatt catatatttg catagaatca gctgaaatta
    19561 tctctgcatt catatctttt gctaatttta ttgacaaatc tgtttttcca acagcagttg
    19621 gacctgtaag tattataagt ggtatttttt tcataacatt cctttccata tattttaaat
    19681 atccaaatat tttacataat tcttttaaac attttttcta tttctgtctt agatatttcg
    19741 accattatag gtcttccatg aggacatgta aatggattct cacacttttc tagctgttcc
    19801 aataaacttt ttatttcaat atcgtatatt ttatcatttg cttttatagc agacctacat
    19861 gccatagatg caaatctctc tccttttaaa tcataattac ttgtaatttc ttctatatta
    19921 tcaattattt gaagtataaa tttctcagtt tcaggtactc caaatatagt aggaacacat
    19981 cttaccatta tatgattatt gccaaaaatt tctagctcaa aaccaaattt cataaataac
    20041 tccaagttat tttcaatttg aagcatatct atatttgata cttcaataac cactgggtct
    20101 aaaagtattt gcatatttat atcttgtcta tagaacttct ccatatatct ttcatatagt
    20161 attctttcat gtgcagcatg ttggtccaac aaatacatag aatcgtcttt gctcaaaatt
    20221 atataagtat taaatacaac tccaattaca aaatatccgt ataaagaaaa cttgcgtttt
    20281 gaactacaca agtattcttc ttcactgtcc ttaatactat ctcctaagta ataatttcct
    20341 tcatttttaa ttccatcaac ttgaaactct tcttgtatat tatcatttgc actatcttca
    20401 cttagataat taatgttttt tacatcatct tctacacttg catttaggac ttcacttact
    20461 gattgaaatt catttattgg tttttcactt gataaagtaa caacttcaat gacttcatcc
    20521 ttacctttag taatattact atttttagga gtagattcta ataaatcatt atttcttaat
    20581 ttataatctg tacttttttc tctactgtta atagcgattc taggttgtgt ttttttatca
    20641 ctataagtag catattttcc aataagattt gagtgtatta atttaacctt taaaaaatct
    20701 ctaagttcta tatacacttc ttgttctttt tcaaatttaa tttcaagttt attggggtgt
    20761 atatttacat ctatacatga tgggtctact tctatattta agaagcacac tgcatgttta
    20821 cctattggaa ttatagactt ataactttct gttattgcat ctatgattat tttacttttt
    20881 acaaatcttt tatttatata gatatgttgt aagtttttat ttgaacggta tatattgtta
    20941 ttacctatat atccattcat tttaaagtga ttgcacttaa actctacatc tattatattt
    21001 tccgttattt cttttccata tatacttcta atagtgttta caagttttcc atcccctgga
    21061 gtatttagca tctgtttatt gttatttgta tatttaaatt gaatgtttgg attacctatt
    21121 gcaagtttat ttatcaaatc acttatgttt atagtttctg catgagtaga ttttaaaaat
    21181 ttctgtcttg ctggtgtatt aaagaaaata tcttttataa tgatggtagt tccattagta
    21241 gaacctattg gttctttcga tattattttt ccaccttcaa catatatttt agttcctatt
    21301 atttcatctt tagttttagt cgtcatttct aattttgata cagctgaaat agatgccaat
    21361 gcttctcctc taaaaccaag agagtataaa tcatacaaat catcaatttt tttaatttta
    21421 ctagtagcat gtctcaaaaa tgatttttca acttcacttg aaggtattcc tattccatta
    21481 tctgtaatct taattagact ttttcctcca tcaattatat ctatggaaat tttatttgca
    21541 cctgcatcaa tggagttttc aataagttct tttactacag aagaaggtct ttctacaact
    21601 tcacctgctg ctatcttatt tatagtcaaa tcatctagga tgttaattat atttttcatt
    21661 tttacacctc taagatttta tttcttttgc ttttctttga agattataaa gagagtttat
    21721 agcatctaat ggagtcatat ttaaaatatc cagatttaaa atttcttctg atagtatatc
    21781 tctatttaca ctatcaaaag agatttgagt taaggctact tcttttacag aatcttcttg
    21841 attaattttc tctttatcat cattttgctt cattaaagca acttcatcat ttaatgcttt
    21901 aaatttagaa tttaatttat cataatcctt ttttatatgt ttgtagtctt ttactataag
    21961 ttcatgttct atttttagag attcatattt attcttaaaa ttactttgat ttaccttaac
    22021 taattcttcg tccaaattac tattaattat attattgttt tctttatctc catttattgc
    22081 aacactgttg taaacatgat ttttttccaa gtctttaagt atatattttg ctctttctat
    22141 gacttcgtct ggtaatttag ctaatttagc aacatatatt ccataactct tgtctgctcc
    22201 ctgaggaatt atttttctta aaaatattat tccttcccca tcttctttga ctgctattga
    22261 ataattttta acctctttaa actcttcttc taaatcagta agttcatgat aatgtgttgc
    22321 aaataatgtc ttacatctaa tatttttttg aatatattcc actattgacc atgctaaact
    22381 tataccatca taggtactag ttccacgacc tatttcatct aatataacta aacttctctc
    22441 tgttgcattt tttaatataa gggatacttc gttcatctct accataaaag tagattgacc
    22501 ttgagataaa tcgtcactag ctccaactct tgtaaatatt ctatctaata ttggaatatc
    22561 agcagactct gctggtacaa atgagcctat atgtgccata agagctatta tagcagtttg
    22621 cctcatatat gttgatttac ctgacatatt aggaccagtt attatgttaa taatgttttc
    22681 tccacgattt aaataagtat catttggaac gaaattttcc tctcctacta tattttcgac
    22741 aacaggatgt ctaccatttc ttatatcaag tttattattt tcatttattg ctggttttac
    22801 gtagttattt atatgtgcaa ctgttgcaag ggacacaaaa acatctatat tagctatagt
    22861 cttagccact ttttgtattc tatctatgtt tttatatatt gtatctctta tctctacaaa
    22921 tatctcgtat tctaaagatt taatcttttc ctctgcatgt agtatctttt cttctatttc
    22981 ctttaattct ggagttatat atctttctgc attactaagt gtttgctttc ttatatatgt
    23041 ctcatctagc tttgcctgtt taaagttggc ttttgttatt tctatataat acccaaaaac
    23101 tttattaaat cctattttta atgatttaac tcctgttttt tctctttccc tgttttctat
    23161 ttcctttaca agaaaggctc cattcttaga aatctctctt agttccttaa gttcatcact
    23221 aaaatctgat ttaattatgt ttccatcttt tatggttatc gtaggttctt ctagaatagc
    23281 ttcatctatt aaattgtata tatcatctaa tttatccatt tctgaaacat actcttttag
    23341 gatttttgca tttgataaat ttatagtatc ttttaaatta ggtaactttt ctatcgaatt
    23401 ttttaagtga attaactctt ttggtgttac tctctcaaat gctatctttc cacatattct
    23461 ttctatatcg tatatatttt taagtatatc atttaggtct tctcttaata taaagtcatc
    23521 ttttatttct tctattacat ctaatctatt ttcaatttta gatttattta tcaaaggttc
    23581 ttctacatat tttcttaaaa gtctacctcc cattgctgta gaagttttgt caagtacatg
    23641 taataatgaa ccttttttct tattccctct tatagtttga gtaagttcta aattagttct
    23701 agtaaacata tctagaacca tatattctga tgaattgtat atatttatat tattaatgtt
    23761 agatgttatt tgtttttgag tattgtatat ataattcaga agaattgata agcttgactt
    23821 tattagacct ttatcatcaa actttagttt ttgtagataa acatctggaa aatattcttt
    23881 taatatattt atatccaaat agttatcact aaaactttca tttatgtata tattacttac
    23941 agtagctata tctctaagct tttctataaa gtctaaatca tttataatta tttcagttgg
    24001 atggattttt gcaatttctt caattacctt atcttcattt aaacatgttg cattagtttc
    24061 tcctgtactt atatcaacat aagttagacc tatattagta ccatccttat acaaagataa
    24121 taaataatta ttctttttat tttcaagtaa atttccatct aaaactgtac caggagtgat
    24181 gacccttata acctctcttc taacaattcc ttttgctgta gatgggtctt ccatttgttc
    24241 acctattgct actttatatc cattttcaac taattttgat atatatgaat tggctgagtg
    24301 gaaaggaaca ccacacatag gagcacgttc ttctaaacca caagcttttc ctgtcaatgc
    24361 tatttcaaga gcttttgaag ctactaatgc atcttcaaaa aacatttcat aaaaatctcc
    24421 aagtctaaaa aacagtatac agtctttata tctatttttt acttcaagat actgtttcat
    24481 cataggagtt aatttattca tatctatttg cattatcaaa cctccttctt ttctaattaa
    24541 atattatatc atagtagatg attattatta acacaatata taaaattttg acatataact
    24601 aaaaacaatc caaaatgtat actcacattt tggattgcta agtttaatgc ttattaaata
    24661 aaaatatttt tttttacaat aaagatatta tttctttttt aagtttaatg atattttcaa
    24721 ggttatcttc atttaacttt ctactatcta taacaaattc tttctttact attgatggtt
    24781 tttttgaaat aacataaatt ctgtttgata gcattatagc ttcgtcaata tcatgagtta
    24841 taagaagtat tgttgaattt acttttttct taacatctaa aagccatttt tgcatggatg
    24901 ttttagttaa tgaatcaagt gccccaaaag gctcatctaa aagcattatg tcattagaat
    24961 ttataaaagt tctcaagaag ttagctctct gtctcattcc tccagaaagt tctgaaggat
    25021 atttatcttc atatccgctt aatccaaaca cctcaaaata aggttttacc attgacctag
    25081 ctttactctt tttttctttt tttaaaatta gtggtagtga cacattatct attatagttt
    25141 tatatggcaa gagtaaatct ttttgataca tataactata cttaccattt atattcactt
    25201 tgccactatc ataatctgta agtttagtta taatattaaa aatagtactt tttccacatc
    25261 cacttggccc taatatagaa acaaactcac cttcataaac atcaatactt atatctttta
    25321 aaacttcaat atctttataa gtttttgata cattttctat agatactttt acttttttat
    25381 tgtggtaaaa attcatttgt gtaagcatca cttgccttca tatccttatc tattaattta
    25441 tattctttta agaatgatgt atagttgttc catacactat ctttcatttc tccccatcgt
    25501 ggtgcatcat ctttgtactt gcttgctaaa tattcttgac tctttaaagc tagtttttca
    25561 tctacttcag gtgcatgttt tactaaaatt tttgctgatt cttctggatt ttttattgca
    25621 tattcataac cttttgttgt agcttttaag aattttttag caagctctgg attattttta
    25681 agcacagttt cattagaaat tataagtggt gtataataat ccaatctctc atctaaatct
    25741 tttactggta taaagtttaa gtcatatcca attaaatcag ctttaactgc atcccaacct
    25801 tcaaatatcc atgcaaaatc tacagttttc atagctgcaa agaaatcatc ttgaccagta
    25861 ttaactatct ttaacttatt aaaatcagct ccatttttct tcataactgc tttaaaaaca
    25921 gcttcttctg atggagagcc ccaaccacca taagtttttc cttcaaaatc tttagctgtt
    25981 gttatattct tttcttttgg tgatgcaaac ccagaagtat tatgttgtat aacagttgct
    26041 acagctttta ttggaagtgg atcttcacta gtctttgcat aagtaacttg ttcttgatag
    26101 cttataccaa agtcagcttt tcctgttgct actaaagttt ctgctccact ttcaggtggt
    26161 tgtactattt caacatccaa accttcttct ttatagtaac ccttatcaag tgctacaaat
    26221 aatccagtat gatttgtatt tggtgtccaa tcaagcacca tagttacttt tttagaagaa
    26281 gtatctttat cattgttttt gtctttattg ttatttgaag aacatccagt tattgttgcc
    26341 aatgtaaaaa cacttactaa acctaatatt cctaactttt taaaattcat aattgcctcc
    26401 ctcaaatacc tttaatctat taatctatgt cttataacaa ctttttttac agcctgtaca
    26461 attcctacaa acattaaact aaatataact actaatatag tagatgcaaa tactttatca
    26521 agtgcatatg cactttttga cctaagcata taaacaccaa gtcctttcgt tccacctagc
    26581 cattctgcaa cagttgctgc cattacagca tacgttgcag atattttaag tccagaaaaa
    26641 aatttatcca ttgccatagg aaatttaagg tgaatgaatg tattaacttt atttgagtcc
    26701 atagtcttaa ataaatttaa ataatcttta tcaatattct caatgccatc tacaaaactt
    26761 attaatatag gaaaaaaaca agtaagcatt accatcaata cttttggcat tgttccaaat
    26821 ccaaaccata tcataaagag tggtgctata attattgtag gtatcatctg tgtaaccaac
    26881 ataataggat aaagacattt ttttattatt gatacaaaat ccatcaatat tccaattacc
    26941 aatgatatta taattgcgac tataaaacca agtattgctt ctcctatagt aaccacagca
    27001 tgtatggata aattttgata atctttaaaa aacacattaa ttatatctac tggtgtgggt
    27061 agtatatact gaggaacttc taaaactcct accatagatt gccaaagaat aagtataaac
    27121 aaaaatgttg ctattggata tatactatct ttgactttat ctatgatttc taagtttttc
    27181 atctatagtc agacctccag ctttttcatc tactctagct actgttatga ctctttcaca
    27241 tccaagatta aaagggattt tatgtacctt ttttaataat tcaaaaactt catcaaaatc
    27301 accttcaaca gtagtgctca ttgctcctat ttcacattta agaccacttt tttcaatttc
    27361 agcaatacaa gcatctacta ctttatacat ctcttcttca cctttgtaag gtcttagtgg
    27421 cattactgct atgtctgcga ttaccattta aatcactcct ttaaaaataa ttaataccta
    27481 ctaggtatat tttgtttttt attaaaaaat tttattacta aaaagatatt atatttaaat
    27541 aaaagaaaag tcttatccaa aataggataa gacttatttt tatcttaact ggtctcccta
    27601 cactggcatt atccaaacag gttcaaaggg tcgagataaa atctcctctc agcacattag
    27661 ctccccgaaa tatattatat cttttctact tgattatact acacttatca tattttaaca
    27721 aattttttat ttatattaga tattttatcc gttcaaaaat atctaatata aattgtacat
    27781 tattacacca aataaattat cttaaaatgg taatttatca acatcttcca aaactagatt
    27841 aactgaattt tctagttttt attttgctat ttcaagcata tttggatgta tatcaattcc
    27901 atacaaatat tgtgctaaat ttctgttttc actcatctaa tttgcaatcg ttacaaatta
    27961 cagattagaa atagctttta gactctactt atctttatct acaaaaaatc cttcaataaa
    28021 ttcattaata atattcagta atctaaaaat aaacctctat ctagattttt aagatacaag
    28081 ccttttagga agtatctttt gtgataatat aaaaattact catcattatc atcaaaatcc
    28141 tcagcaacca acattttaga taagaatcaa actctataat ataattattt tatccaattt
    28201 atttttgaga aataaaatat ttttttaatt tttaaatttt ttaatatgca ccttgaaaaa
    28261 agaaacgttt tcctatatta tggtaatagt agaataaaac taaataggca aaactagaga
    28321 aatttagtga cgcaaagcta tagggactaa gacttatgaa atgagttatg tcagccagtt
    28381 gccaaagaga tattttcttt ttgtttttat gaatgttttt tagggggata tatgatatat
    28441 gtttatggat aaaatggata gatgtacaca tatactaact gcatatatat gcagttcacg
    28501 cgattactgc aattttatag atacacaatt aaatgatttt atactagaat atggagagaa
    28561 tgtggtagaa tcttgtttgc atcaagtgat ggtattggta agcagatata attaaagaaa
    28621 ttttattttc tagaaaaccc tttttagttt tatctacgtt ttataattca ttaaagaact
    28681 aattaaaata agcttcatta tatatttttc ataaagtaaa cagttgagtt tatctcatat
    28741 actgtttacc ccaattacat aattctaata atatagattc taatgattta ccacgtttag
    28801 tagacaaata ttcaactttt ggaggaatag tcggataaga aatcctctta ataagtccat
    28861 ctctttcaag atactttaat tcttgtgaca gtatcttatg acttatatga acaacttctt
    28921 tttttaattg actgtaacgt attggctctt gttttaaaat atgccataaa ataattgccc
    28981 tccatttact tcctatcaca gtcatggtaa aaccaattga acaattaaaa tctttttgtc
    29041 tttcttcaat atttggaaat tcttcaaaat tattatctat atccatctca atcctctctt
    29101 tcaaaatagt aagtatctaa ctaaaaagtg cattcttgtt tagcgtacta attcgtaata
    29161 taatcttgat tgtagtatag agccttacat aatactactt ttttagatgt atatgtttaa
    29221 actttaatat agacatatgt tattttatta attttattta aacagataat aataaataat
    29281 aatttatctc aagttttcta tgtagataaa ctaatctaat gtttagaaag gattatataa
    29341 tatgaataaa aaatttcgtc tttatcaagt tgactcatta caaaagaaaa atttacaggt
    29401 aatccagctg gtgtaattac aaatgctgat ggcttatctg aatcacaaat gcaaaaaata
    29461 gcaagagaat taaataattc agaaactgct tttattttca ataacttggc taaaaatcat
    29521 caagaaaatt atgatgtaca tatcagattt tttacactaa ctaatgaggt tcctatatgt
    29581 ggtcatgcta ctattgctgc ccattatgtt agagcagtag aaaaccaatt caataacaaa
    29641 acagtatttc aaaaaactag tgctggaata ttaccagttg atattataaa aaaagataat
    29701 gattatgaaa taattatgac acaaggaaaa attgaagtat ctccaccgtt acaacaaacc
    29761 tatcaaaaaa gattacttaa tgcatttggt attttaaagg aagatattcg tacagattgt
    29821 cctattgcca tatcatctac aggacattca aaagttatga ttggaataaa ggaattatca
    29881 acattaaata gtttatctcc aaatttatat gaattatcga aaataagtca agaaataaaa
    29941 tgtaatggat attatatttt tacattacat aataaagaaa ctctacaagt tcatggaaga
    30001 atgtttgctc ctgcaattgg tatttcagaa gaccctgtta caggaaatgc taatggaccc
    30061 ttaggtgcgt accttgtaca ttataaagta atacctacac ccaacacaga gtttatattt
    30121 gaagcagttc aaggagaaac aataaaacgc aaaggcaaga tgttagtaaa agtaattatt
    30181 ttagataata aaccagttga agtaaaaata attggaaaag ctgtaattgc attctcaaca
    30241 aacatagaaa tctaattata gattccttct aagtaaattt ttgaactttt taatactaat
    30301 ttaataacaa actaatttat atacaatcaa attaattttt ttattatttg ttaatatata
    30361 tttatcatta tattatataa taatatagat agttactata tcttataatt agataaatca
    30421 taattatata ttattgttaa aactcaatta tataattttt aataatatta ttttaggagg
    30481 taaaaatgat aaacgatatt aaaaataaat tcaaaaatta caaaccatat ataaatggat
    30541 acaagaatat gaagagagca tctgttttaa ttccaatagt agaaataaat aacacacact
    30601 atatattatt tgaagttcgc tcaaaaaatt tgaagcatca acctagtgaa atttcttttc
    30661 caggtggtaa aatagaatca ggtgagactc catatgaagc tgttattaga gaaacttgtg
    30721 aggaacttgg aacttttagt gacaatatag aagtaatatc aagtcttgac ctccttataa
    30781 caccagtaaa gtttatcata catccatact taggatatat aaaaaatatt aataatttaa
    30841 atataaacaa ggatgaagtt gaccatactt ttttggttcc tataaaatat cttttagaga
    30901 atcctccaaa tacttatagt aatactgtaa aagtttttcc agacgaaaat ctaccatatg
    30961 gttcaattcc aaaccaaaaa gattataaat ttgaaagtgg aagttatcct attctgtttt
    31021 ataaatataa agattatgta atttggggaa tgacagcaaa aatactagaa aactttttaa
    31081 attttttatc agaataacac tcataataaa tcatcaaaac ttttacataa gaaatttata
    31141 aaagtttcta aatttaacaa ttctaaaata tcaataacta tattttgtag cactttttta
    31201 ttttattatt gtttgaatat ttgatggttt tcaatgcaat ttcaacataa atactatgta
    31261 ttagtgtata ctgttgatga attaaagtat gataatctat ttaataaagt atgaagtaaa
    31321 tgattttaat gtgcctttat ggtaaaatta ctaataagta ataaaattaa ttaaaatata
    31381 tggaggtgct tataatgttt gctgtaggat ttgttgttat aattgcactt gcctttttca
    31441 ttttaagtgt actattattt aatggaaaag ccagtatgct actagcaggt tataactcta
    31501 tgagtaagaa agaaaaagca cgatacgata aaaaaaaatt atgtagaagt tctagcattg
    31561 ttacttttat tgtctctata atgctattta ttatggcgtt tttgggatat agaatagaga
    31621 gtggtcaaat ggacgaaaaa aaaatggttc ccttttctat aatatttata atagtcatac
    31681 tttccagtgt attttttaat atcctctact ctaacaaaaa atgtaaaaaa taaatagttg
    31741 taagttgagc agttaattat aaaagattga ctgctttttc atgtatattg actactctta
    31801 ttaaatattt taacatttaa aaatctatgt aaaaataatg aatatagtac aaacttctaa
    31861 taatgatttt acctttgtgg ttacagtcca ctatatataa tttaatatga taacagggaa
    31921 ttactaatga caactaggta agcttttata aaatataata aaacaaaaat agatgtacaa
    31981 tgaaattata tcttatatca aatttatttt tattaatagc aaaaacttac attagcataa
    32041 catatgacaa attcgcttaa aataaaaatt tttaatataa tttgttctat agtacatcta
    32101 ttttgattag tttacataat tttattattt tgtacaatat taattattta ctaatatccc
    32161 atttaatgaa aaagttctag gttcagtaat ttttaccata actaatttac caattaaatc
    32221 atcatttcca ccttcgaaat ttacgagttt attttgtcta gttctaccag caaacttatt
    32281 ttcattattt ttacttcttc cttcaactag aacttcaact attctatctt tatatccatc
    32341 atttatttct gcagaaatcc tatttacagc ttctaacacc ctattaaatc tatcatgctt
    32401 tatatcttct ggtatttgat tttccatttt agcagcagga gttccctgtc ttttagaata
    32461 aataaatgta aaagctgaat catatctaac tttttcaacc acatctaaag tgtccaataa
    32521 atcttcttct gtttctcctg ggaaaccaat cattaagtct gtagaaaaag caatacctgg
    32581 aacttccttt ttagcttttt ctattatttc taaataatat tcctttgtat aatgtctatt
    32641 catttttttc aaaaggctac tgcttccaca ttgtattggt agatgtaaaa actcacacac
    32701 cttatcacaa tctctaattg cgtatattac ttcatcagat atatcttttg gatgagatgt
    32761 cataaatcga accctttcaa ttccttctat atcattcact tttcttaaaa gttctgagaa
    32821 tgaaactgga ttttcaagtg tcttaccata agaatctaca ttttgtccaa gtaaagtgat
    32881 ttctttcgtt ccatttgcaa caagctcttt tatctcgtta attatatctt caggagttct
    32941 acttctttct cttcctcttg tgtatgggac tatacaatat gtgcagaaat tattgcatcc
    33001 atacattata tttacaaatg cttttaattc aaattttcta ttacttctaa gaccttctat
    33061 tacttctcca tccacatccc atacatccac taacattttg tcagaattta ttgattctgt
    33121 aagtaattgc ggaaatttat atagattatg tgttccaaat ataaggtcta catgtttata
    33181 tttttttctt aattcctcta caacatgtgg ttgttgcatc atacatccac atacagctat
    33241 tttcatatct ggattttttc cttttagatg ttttaattgt cctaagttac catacacttt
    33301 tagctctgca ttttctctaa ctgcacaggt gttatatatt attaaatcac tttcttcaac
    33361 catcattgac atttggtatc ccatctcttc taacattgaa cataattttt ctgaatcatg
    33421 ttcattcatt tgacacccga aggtttgagt aaacacgagt tttctctttc cagttctgtt
    33481 gaagtattct tcattttgtc tgtgtatttg ttctatatat ttatcttgtt cttgtatttt
    33541 ctctataggt attgttactt gttttctttt gctcacgtct attcctccta gttaatttct
    33601 tatattacac ataaccttaa ttatatcaaa taagaaaaat cttttaaaga tattattatt
    33661 tttctagttg tgttttaata gtaaatctaa tgatttatat atactatatt tctaaaatat
    33721 aaaatcatat aattattata ataataaaaa gatgttttaa attaaaactt aaaacacctt
    33781 tccttaaata aaatatatga tttactatta aatacaatta catctattca ttttcaatta
    33841 aataacaatc atttaatatt aaatattgat cttcctcaac cgttatatat cttcttcctg
    33901 aaaaagtatc attagtaact attccctctg cattacctaa gctatcattt ctaacttcat
    33961 aatatgcatc agaagaattt gacattataa tatattctcc agcaggaatt tctaatccaa
    34021 ttttatacat accatttcca aacttaccat tcttggcctt ctgggcttta gcatactttg
    34081 ctaatgtcat tgttgaatcc tcaattttaa tatattgacc attctttaag gtgatatatc
    34141 tagtcccact aaaaatatca tttgaaagta tactatcagc attacctgta ctatcacttg
    34201 taacttcata ataagaagca taacttcctg agtttgaagt tattaagtat tcacctgctg
    34261 agatatcttt tccaacctta tacattccat cttttatagt ctttgtagta ggtttaacat
    34321 tttttacaac cccagtatta atatctccaa ttccatttgt tatatttaaa cactgttgta
    34381 ctatatttga atctatatat cctatagaag ttatcttctt agcatttttt aaaatgcttt
    34441 tattgctacc atcgttaact aaaaccattg gaccatgttt tgataaagaa gatcctacaa
    34501 gtgcatttgt taattcataa gctccagcta tataaaactc tctagcatcc ttataaaact
    34561 tatttattat tgctttatta gtttcaaaac gagtactccc ccctagtcta gtagatttag
    34621 tattgtttac taaagtggta ctcattgaag ctgttcctcc tattacataa gactttaaac
    34681 cactagttga aaaaggtata ctttgtccat tagtaagaat tattggtgct ttatctcttg
    34741 ctgctacaga tgcaatactt atagcatctg cttctccttt atatgcattg gttagcataa
    34801 cagtattgac tttttttatt gaatttattt cttttgcaac attataactt gtttttattc
    34861 tgtcatttcc cttaattctt acaactttca ttttcttaga ttttaatgaa ttttcgacag
    34921 atgtactaat agaatatgtt cctccaatta tataaacttt tgatacattt tttaatcttg
    34981 cagatgtctc agttggtata gtattttttt cagttaatag tataggggca tttaaagctc
    35041 ctgctagtcc acttgcactt aatccatctg caatagagtt actagtatta attaatatag
    35101 cagttttata tgcagtttta tatgcagttt tatatgcatt tttatctgct attttacctg
    35161 ctatttcata cttatttttt ccttgtattt tttctacaga tgatattgca tctactaatt
    35221 gaatatttgc aaatacaaaa gaaatactta aagtcaatgt taatattctt ttatagaatt
    35281 tcataataaa attgtcaccc tcataataaa tttttaatat ataaatacat attcttaaat
    35341 tagttatgta cttaacaaac ttatgtaaaa ttgcaaatta
//
LOCUS       DBGJFU010000028        35153 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.028, whole genome shotgun sequence.
ACCESSION   DBGJFU010000028 DBGJFU010000000
VERSION     DBGJFU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.028"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            135..311
                     /locus_tag="ACOIAS_001337"
     CDS             135..311
                     /locus_tag="ACOIAS_001337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701053.1"
                     /translation="MNLKKKEKIVKPVLKKLTEIVLNILSKKILNYAIYAISVLIVTK
                     VVINIDLYISILYY"
     gene            412..1149
                     /gene="cas6"
                     /locus_tag="ACOIAS_001338"
     CDS             412..1149
                     /gene="cas6"
                     /locus_tag="ACOIAS_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HGZ2701054.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            1235..2629
                     /locus_tag="ACOIAS_001339"
     CDS             1235..2629
                     /locus_tag="ACOIAS_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701055.1"
                     /translation="MDLKNELEKNDYKLKFTCELNRNYLSLESAEYIDPKYISDYILE
                     NQLFKVFKNGKKETLEKIFSNIDNLTYVNFIEELDKSSATEKQKEGIKKDFKNRRFPY
                     VRNSGKYGLNTSLENMEKNVKRIVDIVIKSNIEIDEIDKLDLKQYEEKDSVCNVCNIN
                     KTTKLDIDLRERKDSKYNFLFRGAEKSGFKRSGQVESNICFECEFFNLMCLLYINLKR
                     PMVFAYTNDLRELAFLNHKIMLKRKMYSDKSFYKKLLHEKISSIRLYRIDIDTNKGII
                     LKFDSIIEYKELLKKIELIDIIDNYNFSREPMNTRNLGKKMINNGNLSNLKELLLDNI
                     SVLRQLTGTSREMDFVSSSYNIGLYIKLCWIVDGREEYKMKNYIESNLIYSRVGKDLF
                     NKMTDESRRKNFSMKVIQLLKSNDRTQLFQTIMHVLVSNGILIGEGFVEGLMQSSELE
                     LNTNVGLFIQEIMK"
     gene            2649..3665
                     /gene="cas7i"
                     /locus_tag="ACOIAS_001340"
     CDS             2649..3665
                     /gene="cas7i"
                     /locus_tag="ACOIAS_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HGZ2701056.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            3681..4478
                     /gene="cas5"
                     /locus_tag="ACOIAS_001341"
     CDS             3681..4478
                     /gene="cas5"
                     /locus_tag="ACOIAS_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HGZ2701057.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4505..6913
                     /locus_tag="ACOIAS_001342"
     CDS             4505..6913
                     /locus_tag="ACOIAS_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HGZ2701058.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7355..8302
                     /gene="prmA"
                     /locus_tag="ACOIAS_001343"
     CDS             7355..8302
                     /gene="prmA"
                     /locus_tag="ACOIAS_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGZ2701059.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8321..9079
                     /locus_tag="ACOIAS_001344"
     CDS             8321..9079
                     /locus_tag="ACOIAS_001344"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGZ2701060.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            9081..10379
                     /gene="mtaB"
                     /locus_tag="ACOIAS_001345"
     CDS             9081..10379
                     /gene="mtaB"
                     /locus_tag="ACOIAS_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061712 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HGZ2701061.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10422..10772
                     /locus_tag="ACOIAS_001346"
     CDS             10422..10772
                     /locus_tag="ACOIAS_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HGZ2701062.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10935..11114
                     /gene="rpsU"
                     /locus_tag="ACOIAS_001347"
     CDS             10935..11114
                     /gene="rpsU"
                     /locus_tag="ACOIAS_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGZ2701063.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            11144..11587
                     /locus_tag="ACOIAS_001348"
     CDS             11144..11587
                     /locus_tag="ACOIAS_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HGZ2701064.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11698..12195
                     /locus_tag="ACOIAS_001349"
     CDS             11698..12195
                     /locus_tag="ACOIAS_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HGZ2701065.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12388..12951
                     /gene="raiA"
                     /locus_tag="ACOIAS_001350"
     CDS             12388..12951
                     /gene="raiA"
                     /locus_tag="ACOIAS_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGZ2701066.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            13164..13400
                     /locus_tag="ACOIAS_001351"
     CDS             13164..13400
                     /locus_tag="ACOIAS_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HGZ2701067.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13413..14756
                     /gene="yqfD"
                     /locus_tag="ACOIAS_001352"
     CDS             13413..14756
                     /gene="yqfD"
                     /locus_tag="ACOIAS_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HGZ2701068.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14695..15810
                     /locus_tag="ACOIAS_001353"
     CDS             14695..15810
                     /locus_tag="ACOIAS_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGZ2701069.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15867..16328
                     /gene="ybeY"
                     /locus_tag="ACOIAS_001354"
     CDS             15867..16328
                     /gene="ybeY"
                     /locus_tag="ACOIAS_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGZ2701070.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            16334..17053
                     /locus_tag="ACOIAS_001355"
     CDS             16334..17053
                     /locus_tag="ACOIAS_001355"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGZ2701071.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            17172..17573
                     /locus_tag="ACOIAS_001356"
     CDS             17172..17573
                     /locus_tag="ACOIAS_001356"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGZ2701072.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17637..18530
                     /gene="era"
                     /locus_tag="ACOIAS_001357"
     CDS             17637..18530
                     /gene="era"
                     /locus_tag="ACOIAS_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGZ2701073.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18542..19921
                     /gene="mgtE"
                     /locus_tag="ACOIAS_001358"
     CDS             18542..19921
                     /gene="mgtE"
                     /locus_tag="ACOIAS_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HGZ2701074.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19994..20149
                     /locus_tag="ACOIAS_001359"
     CDS             19994..20149
                     /locus_tag="ACOIAS_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HGZ2701075.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            20115..20888
                     /gene="recO"
                     /locus_tag="ACOIAS_001360"
     CDS             20115..20888
                     /gene="recO"
                     /locus_tag="ACOIAS_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGZ2701076.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20881..21480
                     /locus_tag="ACOIAS_001361"
     CDS             20881..21480
                     /locus_tag="ACOIAS_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HGZ2701077.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21640..22518
                     /gene="glyQ"
                     /locus_tag="ACOIAS_001362"
     CDS             21640..22518
                     /gene="glyQ"
                     /locus_tag="ACOIAS_001362"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGZ2701078.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22518..24584
                     /gene="glyS"
                     /locus_tag="ACOIAS_001363"
     CDS             22518..24584
                     /gene="glyS"
                     /locus_tag="ACOIAS_001363"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGZ2701079.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(24750..26297)
                     /locus_tag="ACOIAS_001364"
     CDS             complement(24750..26297)
                     /locus_tag="ACOIAS_001364"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HGZ2701080.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26577..27344
                     /locus_tag="ACOIAS_001365"
     CDS             26577..27344
                     /locus_tag="ACOIAS_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGZ2701081.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27627..27824
                     /locus_tag="ACOIAS_001366"
     CDS             27627..27824
                     /locus_tag="ACOIAS_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2701082.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27853..28929
                     /gene="vorB"
                     /locus_tag="ACOIAS_001367"
     CDS             27853..28929
                     /gene="vorB"
                     /locus_tag="ACOIAS_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HGZ2701083.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28932..29651
                     /locus_tag="ACOIAS_001368"
     CDS             28932..29651
                     /locus_tag="ACOIAS_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HGZ2701084.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29664..30203
                     /locus_tag="ACOIAS_001369"
     CDS             29664..30203
                     /locus_tag="ACOIAS_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HGZ2701085.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            30360..31418
                     /gene="buk"
                     /locus_tag="ACOIAS_001370"
     CDS             30360..31418
                     /gene="buk"
                     /locus_tag="ACOIAS_001370"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HGZ2701086.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31436..32329
                     /locus_tag="ACOIAS_001371"
     CDS             31436..32329
                     /locus_tag="ACOIAS_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HGZ2701087.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            32359..33567
                     /locus_tag="ACOIAS_001372"
     CDS             32359..33567
                     /locus_tag="ACOIAS_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2701088.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33591..34850
                     /locus_tag="ACOIAS_001373"
     CDS             33591..34850
                     /locus_tag="ACOIAS_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2701089.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        14211 a         3554 c         6482 g        10906 t
ORIGIN      
        1 tatcgatttt atttaaataa ccccatattt attaattatt agaaatatat tttaattatt
       61 gactaaataa taaatactgt aatataatgg aaatacagtt taataaatat aaaaagagaa
      121 agagggaatt ttaaatgaat ttgaagaaaa aagagaaaat agttaagcct gttttaaaga
      181 agcttacaga aatagtatta aatatattat caaaaaaaat attaaactat gctatatatg
      241 caatttctgt tttgatagta acaaaagttg ttataaatat agatttgtac atatccatat
      301 tgtattatta gatatcttat agatataaaa aattaataga tatatatcta ttaatttttt
      361 atattacaaa tagagaataa tgaattatac tagaaagtag gaggtgaaaa tatgagcaga
      421 atttcaattt ttatggaatc atgtaatggc aaatttccaa atgaaaattc aatgttgtca
      481 gtttccttta taaaaaatat tttaaatgca gaaaacaaag attttgctaa aaacatattt
      541 aactatgggg aagaaagaaa aaacaataag caaataaagg atataaatac agccatttac
      601 ataccaaatc ttataagaag caaaaatgag ttacttgttg atggagatat aatattcaat
      661 ataagttttt ataattattc tatgttcagt aaattataca atggaatttt aaaagtaaag
      721 gagctagaat ataaaggtta tagatttaaa ataaaaaata taaaaataca taaagaacac
      781 gagataaaag aaaatggtgt tatatttaaa actatgtctc ctataatagt aaagaataaa
      841 gaaggtaaat atttagatgt agaagattca aattatatag aatgtttaaa ctatatagct
      901 aatctaactc tggagagtat aagaggtagt gggcttagaa agccattgga atttatacct
      961 cttaacttca aaaaaagagt gttaaaagaa aagattcgtg gatttaaaga gagagaattt
     1021 tactatataa atgcttatgc aggaacattt tttttaaaag gaaatatgga agatttaaat
     1081 gcactgtata aaatgggaat tggatataga aggacggaaa atgcaggaat ggttgatata
     1141 ttaaagtagc gaggtgagag aaataaaagc taaattttat agatcaggtg atattgtaag
     1201 agatattggc attgtttact tttgtgaatt actaatggat ttaaaaaacg aactagaaaa
     1261 aaatgattat aagctaaaat ttacttgtga gttgaataga aattatttat ctttagaatc
     1321 tgcagaatat atagacccta agtatatatc tgattatata ttagaaaatc aactttttaa
     1381 ggtatttaaa aatgggaaaa aggaaacctt agaaaaaata tttagtaata tagataattt
     1441 aacatatgta aattttattg aagaattgga taaatcttct gcaactgaga aacaaaagga
     1501 agggattaaa aaagatttta aaaacagaag atttccatat gttagaaata gtggtaagta
     1561 tggattaaac acgagtctag aaaatatgga aaaaaatgta aaaagaatag tcgatattgt
     1621 gattaaatca aatatagaga tagatgaaat agataaactt gatttgaaac aatatgaaga
     1681 aaaagatagt gtatgtaatg tttgcaatat aaataaaaca acaaaactag atattgattt
     1741 aagagaacga aaggactcta agtataactt tttatttaga ggtgcagaaa aatcaggttt
     1801 taaaagaagt ggacaagttg aaagtaatat ttgttttgag tgtgaatttt tcaacttaat
     1861 gtgtctttta tatataaatt taaaaagacc aatggttttt gcatatacga atgatttaag
     1921 agaattagca tttttaaatc ataagataat gcttaaaaga aaaatgtatt cagataaatc
     1981 attttataaa aaacttttgc atgaaaaaat aagttcaatt agactatata ggattgatat
     2041 tgatacgaat aaaggtataa ttctaaaatt tgactctatt atagagtata aagaattatt
     2101 aaaaaaaatt gaacttatag atattataga taattataat ttttcaagag agccaatgaa
     2161 tactagaaat ttgggtaaaa aaatgattaa taatgggaat ttatcaaatt taaaagaatt
     2221 attacttgat aatataagtg ttttaaggca actaactgga acttctcgag aaatggattt
     2281 tgtatctagt tcatataata tcggtttata tataaaattg tgttggattg tagatggaag
     2341 ggaggagtat aaaatgaaaa actatataga aagtaattta atatattcaa gagtaggtaa
     2401 agacttattt aataaaatga cagatgaatc cagaagaaaa aatttttcta tgaaagtgat
     2461 tcaactactt aagtctaatg atagaactca gctatttcaa actataatgc atgtgttagt
     2521 ttctaatgga atcttaatag gagaagggtt tgtagaaggt ctaatgcaga gttctgaatt
     2581 agaattaaat acgaatgtag gtctatttat tcaagaaatc atgaaataaa aaagggggaa
     2641 aatttaaaat gaatgaaaaa agagtaaaag gagtaacatt aactatactg acagaaagcc
     2701 cagttccact ttcgtatgac caagggtatg gaaattatac accaataaaa aaagaacaat
     2761 atagagataa agtacatgca aaaactagta tagctacaat aacatatgat ttaagaagaa
     2821 tactccatca agaatatggt tggaatttaa gtaatattgt ttttggaaaa aaaggaaata
     2881 tatatccatc tattaagaaa aatgtaggat gtgtagatga aaatggatta gagactgatg
     2941 tatttggtta tttaatacca cttaaagaga aaggttctat aagtaaggct tctccgctta
     3001 gaataatacc ttttaggtca ctaaattcat atagaggttc aacacagttg ataacaaata
     3061 gaggtttttt aagtagcgaa tttggtagaa aatattatga tgagagggaa gaaaatgaag
     3121 tacttagaga tgaaaacttt ccaacaacac aagcattagc aatagaagag actttatcag
     3181 attactacgt ttatacaata acattggagc ttgatagaat aggagtagta gaagtagaag
     3241 aagatggaaa gttactttta ccagaagaaa gaaaatttat gagtaaggag ttaagagaaa
     3301 aagcagtaaa agatatatta gatgcaatta ctgtgtttac aagaaacata aaacattcat
     3361 cagtactatt aaaaccactt gcagtaatgg gtggagcttt tgataaagtt gtaccatttt
     3421 tttgggatga tgtagattat aatgctgata gtggtgagat aaatttagag ggagtcatag
     3481 aaactataga aagttatagc cttaaagaaa gtaacacaat acttgctatc aatgacagat
     3541 taaaaatttc taataaaaag gaattaaata agtttaattt aagtaaatac cctgtaaaag
     3601 agattaaaaa tctagcagat agattggaaa ttggagaaga taatatgtgg tatttaaaag
     3661 agtaggaggt tttttgaatt atgaaagtat tacaatgtaa acttaagcaa ccaactgctc
     3721 actatagaga cccaaaagta tttcaaaatg aatatataag tactttaaat ttaccatcta
     3781 agaccacaat aatgggaatg ataacgtatt tatgtgatag aagattaaac tctgatatag
     3841 atataggaat aattggaaca catcatcata gagaactaga attttcaaga ggagagaaca
     3901 ttgatttttg gaatgaatat acaaatatga gaaaaggcaa agataaagaa aagtttttgc
     3961 ttcaaggaaa ctactatgat tactataaag aacataaagc tcaaaatagc atattaaact
     4021 atgaagttct taaagaagta gaactgacta tatttatttc ttgcaaggat gatgaagaac
     4081 ttgaatttat tagaaaaaag cttgaaagtc catgtaaata tgcaaattta ggaagaaaag
     4141 aagattttgt aataccaagt gaaaaagggt gttttgtgaa agaagttgag ttaaaagagg
     4201 ttatacctat gaatactcgt gatgctataa aagaaaatat aaaattaaag aatacatatg
     4261 taagagttga cttaagagat aaagagaatg tcgagactat aataaatcaa ggtgtattga
     4321 ttgcattacc ttataaatat aaagatttag aagcaaatag agatgataga gtttatgagt
     4381 tttgccatta tatatatgtt gataatgatg gtatatatcc taaaaatatt aaggtaaatg
     4441 tatgtattga tacaaaagaa gtttttacat ggctttaaat tattttgaaa gataggtgtt
     4501 tatcatgaaa aatatcatgc aaaaagaaat attagctaag tcatataatt taagaactaa
     4561 tcaacaagag caaacattag ttgaacatat taaggattta tttgaagtat tagaatctat
     4621 attagaacta aatctatatt cagataaaga tgtagagata ctaaaaatat gttgtgcttt
     4681 gcacgattta ggaaaaatta attcaattat gcaacaaaaa atggagatta ataataaaat
     4741 atcgtatagt tgctcagaag aagagaggaa aaagttagag tctgacaaaa aatctttaaa
     4801 aaagatagca agacataata tattttctgg agcattttta aaagatatat tagaaaaaat
     4861 gaacttgtca gaagaagata agttatatat atataaaagt ataatgttac atcatggaaa
     4921 ctatgaagat tatatgaggt taagtattag taaagttcaa gaagaaattt atgagtatat
     4981 tgaaaaaggc attttagaaa aagaagagtt taatctgaaa gatatagaaa gttatataaa
     5041 tagcatttta aatgtagact ttaaatttgg tgaagatgta ttggattatg attacataga
     5101 taaattaagt gaaagtatgt ttatagacag tgattataac aatggtcaaa tagatgattc
     5161 agttttaaat tatagaaagt ttaagtatat tttatataaa gggatgctga acttaataga
     5221 ccatagtgca tcaagtagac agaaaggtat aaaattttat aacgatttta ctgatgaaga
     5281 aattgataat atgattttaa atgagatata taagagtcag ggtaatttgg aaaaaaataa
     5341 tatagagttc aatactatac aaaaaaggtt gagagaactt tctggaagaa atgttctgac
     5401 tgttgcattt acaggttcag gaaaaacagc tgcagattat agaaagactt ttaaaagaaa
     5461 attcttttta gtgccaaata aaatatcagc agaaagtttt tatagaaaaa atatttttca
     5521 aaataaaaat gatttagata caagaagtag taatgattac attggattac ttcatggaga
     5581 tataaactta tattcagaga atgaagataa tggagaacat gattttgtat tgacattaag
     5641 ggatatagat ttgagtataa acttttgtaa accttgtgta attgcaacag ttgaccagtt
     5701 attacttagt atgtttaaat ttccgggata tgaaaaaata ttcgcagccg taaaaaatgc
     5761 atctataact gttgatgaag tacatttatt ggaaccaaag atgtttttga ttttgatata
     5821 ttttatgcag tttgcatgca agtacttaga tgttgatttt cacttaatga cagctacact
     5881 tccaaaatca tataaagagc aaatgataaa caagggaatt atttttcaag aagaaagtaa
     5941 tgaaaatgtt actgataaag gagaaatagt ttttattgag agcaacaagg aagaagagat
     6001 ttgtgaaggt aaagatgtaa aggtttcttt tataaaggaa aaagaaatca agtcaatagt
     6061 tgaagatgct ttagaaaata aacaaaaaat tcttataata aagaatacta ttgattcagt
     6121 caatagaaca tatgagtttt taaaagaaaa tttatctgat aagtatggtg gtgtagatat
     6181 agatgtttta catagtagat ttaagttcaa agataaaaaa gagaaatata gtaaaatatt
     6241 aaatggagag ggtgatattt ggatatcaac tcaatcagta gaaatatcct tggatttgga
     6301 tttcaatata ataataagtg atttagcaac tatggatagc ttgatacaaa gaatgggtag
     6361 atgtaataga aataataaat atgaatatgg aaacttttat attctaccta gtgaagataa
     6421 aatttatgat aacaaactta agaacacaac taaaaacatc ttaaaggaca tattaaaaac
     6481 taactctatt tttacaatgg gaattagaaa aactattttg gatgattact atgataatag
     6541 tgtagtcaaa aaatattttg aagagaattt tataagttgt gataaagaaa ttaagaatat
     6601 ttatggtata aataaagaaa tttttgatgg attggattta atttttaatt ttgaacctta
     6661 caaaaatatt gttgatagta agaaggaagc agctaaaatt tttagagatg ttgatgttag
     6721 ttacaaaatt attctggaag aagattttta taaggaagat agagagttac aacaagattc
     6781 gattcaagta tcagggttta tatttaatag attatattat tatgggttaa taagcaaagt
     6841 agaaggatac atggtactaa aatctagtag taagtttgag tataatagta ctgtaggctt
     6901 aattttgaag tgagcatatg tttaaatata atataccttt agacattgaa aatacaaaaa
     6961 gtactactgt aagctcactg taaaaactat ttttttaaaa ggaaaaataa gttgtgagat
     7021 agagtgttta caagtgtata tagataataa atttgaggtt taagattaac taagtgggtt
     7081 tcaggtacat cgcgaaaata attataagtt ggagattttg tatgtttaag attaactaag
     7141 tggatttcaa atattgatat agtagatata atagaagttt tattattagg tttaaaatta
     7201 actaatcgga tttttaaata ttctgactat tatataagga tattattagc ttgaatttaa
     7261 aaccaataat ataaaacaac aaaatattat atttatgata taataaaagt tatggaaaaa
     7321 caggaatatt actatagaga agagaggtaa acttatgaat aattggatag aagtaactat
     7381 aaaaacaaca acagaagcag ttgaacctat aacgaatata ctatatgaac aaggagctgg
     7441 aggagctgta atagaagacc caaaagactt tttattccaa aagaaaaatg aactagactg
     7501 ggattatgtt gaagaagaag tatttaaaaa gaatgaagaa gatgatgttc ttataaaaac
     7561 ttatgtttct gaagaaaaaa atgtgatgga atttgtcgaa ataataaagc aaaaagtttt
     7621 agggttaaaa gattttggta tagatatagg tgaaggaagt gtatcattgg accaagtaaa
     7681 tgaagctgat tgggcaaatg catggaaagc atactacaaa ccaacaaaag taggacaaag
     7741 ggtagtagta aagcctacat gggaagatta tgctatgcaa gagggggatt taatcataga
     7801 gcttgaccct ggaatggcat ttggtacagg tacacatgag actactagta tgtgcattag
     7861 agaattagaa aagtacgtga ataaagattc taaagtattt gatattggct gtggtagtgg
     7921 aatccttgca atagcagcag ctaaattagg agcaaaagaa gttgtagctg tagatttaga
     7981 tgaagtggct gtaaaagttg caaaagaaaa tgttcttgaa aataaagttg aaaaaagtgt
     8041 atctgtaatg catggtaatt taactgatgt aataaaagat aaagcggatg taatagttgc
     8101 aaatataata gctgatataa taaagatact tgcaaaagat gttcaaaact ttatgaaaga
     8161 agatgctata ttcatatctt caggtataat tttggataaa gtagaagaag ttaaagaaag
     8221 cttaattgaa aatggatttg aaatagttga agttcaaaaa ttaggtgaat ggtcagcaat
     8281 agtttctaag cttaaaaaat aagggatatg gtgacgtata atggatagat tttttgttga
     8341 aaaaaataat ataaatctac aagataaaac ttgcactata gaaggtgaag atgtaaaaca
     8401 tatttcaaaa gttttaagat gcaaattagg tgagaaactt gaaatatgtg acaagaataa
     8461 taatgagtat atttgtgaga taatgaatat agataagagt atagttaatt tagaaatttt
     8521 agaaaaagtt gatataaata gagaatcaga acttaaagta aggctatacc agggacttcc
     8581 aaaagctcca aaaatggaaa tgatacttca aaaattaact gaagtgggtg tagaagaaat
     8641 aatattagtt caaactaaga gaagtgttgt taaagttgat gacaaaaaag aagataaaaa
     8701 atttgaacgt tgggaaagaa taatttatga agcagcaaag caaagcaaaa gaggtaagat
     8761 accaaaatta agaggtgttt taagttttaa agaagcttta gaagatatga aaaaaaataa
     8821 tgtaaatatt tgcccttatg aaaatgaaag aacagtttca ataaaacatg cactaaaaaa
     8881 atgtgattct aatatagata gtgttggaat ttttataggt ccagaaggtg gattttctga
     8941 agaagaaata gaacaaatac aaaaaaataa ttgtaatgtt gtatcattgg ggcctagaat
     9001 attaagaact gagacagctt ctgttgtagc atcaacaata gctttgtatg aactaagtga
     9061 tttaggagga gaaaaataaa ttgaaaaaag tagcttttta tacacttggg tgtaaagtta
     9121 atcaatatga aacagaagct atgctagaac tatttgagaa agatggttat gaacaagtca
     9181 atagtgaaga gtatgctgat gtttatgtta taaatacatg tacagttact catatgagtg
     9241 atagaaaatc tcgtcaatat ataagacgag ttaaaaagaa gaatccagat gcaataatag
     9301 ccgttgtagg atgttattct caggtatctc cagaagaaat tttggatata gaagaagtaa
     9361 atttagtcat gggcacaaat gatagaagaa agattgttga agaaattaaa aagataaatt
     9421 caagtaagaa agttagtaca gttgatgaca taatgaaagt taaagctttt gaagaaatag
     9481 aaatcagcca aactaatgga aagactagag catttatgaa aatacaagat ggatgtgaca
     9541 gattttgtac ttattgtatc attccttatg cccgaggacg cgttagaagt agagatattg
     9601 atagtatagt cgatgaagtg aaaaaattgg ctaataatgg ctataaagag gttgtattaa
     9661 caggaataca tgttgcctct tatggaaaag atttaaagga tagggatatt aaacttcttg
     9721 atgtaataaa acaaataaac cagatagaaa aaatagaaag aattaggctt agttctgttg
     9781 aaccaatttt atttacagat gaatttgtaa atgaagtttt aaagatggat aaagtttgtc
     9841 ctcattacca tttatcactt caaagtggat gtgatgaaac tctaaaaaga atgaatagac
     9901 gatacacaac tttagagtac aaaactattg tagatagatt gagaagtaaa atgccagatg
     9961 tggcaataac tacagatgta atagtaggtt ttcctggaga gacaaatgaa gagtttaaaa
    10021 aaacgtacga gtttttaaaa gaaatagagc tttcacaaat gcatattttt aaatattcac
    10081 caagaaaagg aactccagct gcaactatgg aaaatcaagt tgaccctcaa atgaaacact
    10141 ttagaagtga acaactttta aatttaagta aagtaaattt taataaattt gcaactaaat
    10201 ttatcggccg tgagttggat gttttatttg aacaaaatat agaaggcaat aaatatgagg
    10261 gattaacttc aaattatata agagttgttg ttgaaagtga caaaaatata caaggtcaga
    10321 tattaaaagt gaaaataaat gatgtaaaag atgaatatgt agaaggaatt ttgctataac
    10381 atgtagaaat tataatattt ataagatagg aggtggaaag aatggattgt attttttgta
    10441 aaatagcgaa cggagaaata ccttcaacta aagtttatga agatgataga gtcttagctt
    10501 ttaatgactt gaacccagtt gctccatatc atatattagt tgtacctaaa aaacattatg
    10561 atagtttaat tgatattcct gataaagaga tggacattgt gtcacatatt catgttgtta
    10621 ttaataaaat agctaaagag aaaggttttg accaaactgg attcagagtc ataaataatt
    10681 gtggaagtga tggagggcaa gaagttaaac atttgcacta tcatatttta gctggtaaaa
    10741 agcttcctaa ttacgaagcg ggacaaaact aaactaaatt attgaaaaat tgagttcctt
    10801 cttgataaaa atacttgata aaagtaaaaa tttagtttat aataaacaaa gtgttaggtt
    10861 gtaatgcata ttatgcaaaa agaacatagt tacagtccca gcataattgc gctattggag
    10921 ggagggaaaa agaaatgtca gaagtaagag ttagagagaa tgaaacatta gacagcgctt
    10981 taagaagatt caagcgtcaa tgtgcaatgt ctggcatcat gtcagaagtg agaaaaagag
    11041 agcactatga taagccaagc gttaagcgta agaaaaaagc tgaagcagca agaagaaaaa
    11101 acgctaagaa atagtagtaa ataagaataa agaggtgaag gggatgtccc ttaagcaaaa
    11161 gttacaagag gatttgaaat cttcgatgaa aaacaaagat acagtgagaa aatctgtagt
    11221 aactttaata agagcttcta taaagcagta tgaggttgat aatagggtcg agcttgatga
    11281 agatggaatt atcgatgtaa tagcaaaaca attaaagcaa cgtagagatg cattagttga
    11341 atttgaaaaa gctggaagag aagacttaat aaaagaaaca gaaggtgaaa ttgaagtttt
    11401 aaaagagtac ctacctcaac aattaagcga agaagagtta gaagaaattg taaaatctac
    11461 tatatctgaa gtaggggcaa cttctatgaa ggatatggga aaaataatgt cagttatcca
    11521 accaaaagtt aaaggaagag ctgacggaaa acttataaat aagttggtaa aacaaaactt
    11581 acaatagatt gatatagcct agagagaaat ctctaggctt tttatagttt ttgggaataa
    11641 cgagtcatat ttgagcatat ctataatcaa agaaaatttt gaaagaaggg gaaaaatttg
    11701 attatatcag taaaagagat taggaatttt ttgataaaca tatttatacc actaataata
    11761 ggttacttaa gtagcatctt aacaatgatt atcagtggaa cagatatttc aacctattat
    11821 ttacaactca caaagccaag ctttgcacca ccagctttta tctttcctat agtttggact
    11881 atattgtaca tattgatggg tatatcttca tacctaatat taagaaaggg ttacaactta
    11941 cctaaagtaa gagatgcaat tttttattat ggattacaac ttgtattaaa ctttatttgg
    12001 agtatactat tttttggatt tggacttaga tttacagctt tgatagatat aataataatg
    12061 attttggttt ctataataat gatttcgaag ttttctaaaa tagataagag atctggatac
    12121 ataaatttga tttatttaat gtggcttgtt tatgcaggat ttttgaacta ttttatatgg
    12181 tttattaata aatagaatgt aaagattttg taaattaccc tgtactttta ataatatcat
    12241 aacaaacgaa aaataaatag actattaaaa ataatcattt tcatatacaa taatattaat
    12301 ttaaatacaa taaatttata aaaattgtat ttaaagatta acatattaaa gtattagaat
    12361 tggaaaggct tgaagggaga ggtttttatg caaataatag tatctgggag acaaatgaaa
    12421 ttaactgacg gaataaaagg atacgtagat ggaaaattaa gtaggttaga aaaatacctt
    12481 gatcctgaat cagaagtgaa agtaacagta agtgcgaaaa aggatagaca aaaagtggag
    12541 gttacaataa ttcctattaa cggtcaaata ataagagcag aagacgtaga agatgactta
    12601 tatgcagcaa ttgatattgt ttgtgacaaa ttaagtagac aagttgtcaa atataaaact
    12661 aaagttaaag acaaagttca aaataataag agcataagat ttgaaaatct tgactttata
    12721 gataatagca gtgaatttga tgattatgat tatgatgatg aagaagatga gaatatagta
    12781 atagaaagaa gaaagaaatt taatgtaaaa cctatgagtt ctgaggaggc gatacttcaa
    12841 atggaactag taggacacaa tttctatatg tttagaaacc aagataattt tgaaataaac
    12901 atagtgtata agcgtaaagc tggtggatat ggtttaatag aacaagatta ataaaaattg
    12961 tatacgttta gattttatat aagaaaaaat aaaaaggtaa tcatgttgat tttttatcaa
    13021 tatgattacc tttttcttgt ttaaataata tattagatat taggtattta ttatataaaa
    13081 attttaaaac taatctgtta caacatcaat aaagtgcata aatacctatt tcaagcataa
    13141 aattaattaa gggggtaatg cttatgttag aaatatccac tgatttacaa gtaaatcaac
    13201 cagttattac tgttacaagt aatacgttta ttagtataga aaattatcta tcaatattag
    13261 aatatgaagt tgatttaata agaataaaga ctaaagttaa aacaattaaa atatctgggg
    13321 ataaactttc acttaagtat attactgata gtgagatagg tataaaaggg attatctaca
    13381 atgttgagta tgttgattag gaggagatac acttgataag ttttataagg ggatactatg
    13441 ttatagttgt ggaaggagta ggactcgaac aatttttaaa tcatcttata agaaatggga
    13501 taagtgttta caatgtaact agaataaaaa atactaaaat ggagtttcat atagatagac
    13561 aagatataaa agaatttaag aatgtttata gaggaagtaa gtttgatata aaggtaaagc
    13621 aaaaaactgg ggtccctttt attatcaaaa gagtttataa gcacaaaggt atgtggattt
    13681 gtgctcttgt ttctttattt ttattgatgt ctacatcaca gtttgtgaca gatgtatata
    13741 tacaatctcc tgaaggtata aaaaaagaag ctcttagaaa tgagctttat aaggttggag
    13801 taagaccagg agtctataag aagagtattg atagaaaaga agttagagac catatgatgt
    13861 caaaatttaa tgatgtggca tatttatcta tcaatgtaaa aggaacaaat atatttgtta
    13921 ctgttacaaa aaaggcagaa tccctaaaat ctacagacca atcaaattat tgtaatgtaa
    13981 ttgctttaaa aaatggtata atagaaaagg taatccctag aagtggtaaa tcagttatta
    14041 aatctggaga catagtccaa aagggagatg tcttattaaa tggagctaat accaagtcaa
    14101 taccagaagt atgggcaagt actttttatg agtccacaaa aaaggcaagt tatgtagata
    14161 ctgtaaataa aaaaactgga gaaaagaaaa atatatatac actaagtttt tatgataaag
    14221 agtttacaat gagaaaaaat ataaaatata aagattatgt tgttgaaaat aaagaaaaaa
    14281 aactatcaat aggaaactat acttttccaa taaagataaa aacgagtact ttttatgaaa
    14341 caaaaaaggt tgaagtaaaa aggaataaag aagagttaaa aaaagaatta tctgaaaaag
    14401 ctttaaaaga gttagaatac ataatcccag cttcggctag aataatagat gtaaaacata
    14461 actttaaggt taataaaaat atgttagaat atttaataac agttcaaact agtgaaaata
    14521 ttgctaaaat ttatcctctt agtaagtcag aagcagaaag gtttataaag gaagaaagta
    14581 aaccagatga aggtgaagag gaagttcctt ccaatccaga aaaaagacca ttaaatgata
    14641 taagaaatga gtttgatgag gataataagg ataataaaga tcaaaacaat tctgatgaaa
    14701 ataatagtaa tcaaaatagt aataatagtc aaaataataa tagcaataac aactaataaa
    14761 attaaactta aaatcttagg aggactaatt caattgataa tacaaaagaa atttacaata
    14821 gcagatggta actttgaaag agagttattt ggcaactttg atgaaaatgt aaaattaata
    14881 gagaagactt taaatataga tgtaatactt agagaaggca atataatttt aattggagaa
    14941 gaaaaaaatg tagattctgc attaaaactt atgaatgaat tacaccaaac tgtatcaaat
    15001 ggtaagcatt tagataagca gagtatttct tattcattat ctcttttgct tgaaggaagt
    15061 gaacaaaaaa taaaagaact tgaggggaca atcgtcataa ctcaaagggg aaaagctgtt
    15121 caacctaaaa ctttaggtca aaaagaatat ataaaactta tagaaaataa tgatataact
    15181 tttggtgttg gacctgctgg aacaggaaag acttatcttg cggttgctat ggcagtaaaa
    15241 gcttttaaaa gagatgaagt tagtagaatt atacttacaa gaccagctgt ggaagcagga
    15301 gagagtctag gttttttacc aggagattta aaggacaaag ttgacccata tttaagacca
    15361 ctatatgatg ccttatttga aatgttgggt gctgataaat ttaataagta tttagaaaga
    15421 ggtactattg aggttgcacc acttgcattt atgagaggta gaacacttga taattcattt
    15481 ataatattgg atgaagctca aaatactact tctgaacaaa tgaaaatgtt tttaactaga
    15541 cttggatttg gctctaaagc tgtagttaca ggagatgtaa ctcaaacaga tttaccacaa
    15601 aataagaaga gtggtctaat acaagctaca gagattctta aaggtgttcc aggtattggg
    15661 tctataatgc taacagatag agatgttgta agacatgagt tggtacaaag aataataaga
    15721 gcttatgaga agcacgacaa aagagaagaa tttaaaaaag aagaacgtaa aaaagcgaaa
    15781 atgcaggaaa agactttcaa aagaaagtaa aagtgtagta atatagatat atagcattat
    15841 taatgaataa aaagtaggtg tagtgaatgg atttgatact agatgataga caagataaat
    15901 tagaagtaag tgaggagtta attgaaaaga taaaggatat aatcatagaa tgtttagatt
    15961 atgaaggata tgatgataat tatgaagtga gtctttcttt tgtagataat aaagagattc
    16021 atgaattaaa tagagaatat agaggagttg atagagtgac tgatgtttta tcatttcctc
    16081 ttttaagtga tgattttgag gatgttgagt tagaagaaga atccctaggt gacatagtag
    16141 tatctcttga aagagcctta gaacagagta tagagtataa ccatagcttt gaaagagaag
    16201 tatgtttttt aatatgtcat agtatgtttc atcttttagg ttatgaccac gatacagatg
    16261 aaaatacaaa agaaatgaga gaaaaagaag aacacatatt aaataagtta aacataacaa
    16321 gggagtagtt tttatgaaac cagaaaaaac tcgtcaaggt ataattaaag catttaatgc
    16381 agctatagaa gggatattgt atacatttaa atttgaaaga aatatgaaaa tacattattt
    16441 aggttcagtt gctgttctta taataagttt gttttttaac ttttcaaaat tagaaatgat
    16501 aatgttactt atgtccatat gcttagttgt agttgctgaa atgtttaata cagctataga
    16561 aaaagctgtt gatttagtaa ctgatgaata tcatgttctt gctaagatag caaaagatgt
    16621 tgcagctgga ggcgttctag tggcagcact aaattcagta gtagttggat atattttgtt
    16681 ttatgataaa ttgacagata tatctggaat attaatttat aaaataagag aatcagaatt
    16741 acatataacc ttgatatgta tattattggt tcttatagct gttgtagttg taaaagcatt
    16801 gacttctaca ggtactcctc taaaaggagg aatgccaagt ggtcatgctg ccttagcatt
    16861 tgctatagca actgcaatta cacttatgac tgaaagagtt gttgcctcta ctcttgcata
    16921 tataatggct gtattagttg cccaaagtag aattgaagga aaaatacata ccttttggga
    16981 gactattgct ggagctttgt taggtgtgtt gatagctata ttagtatttc agcttggaat
    17041 gttctataat taaaataaat gtacataaaa tatttaaaat aaaacaaatc tgtgctaatt
    17101 aaatttttat gatataatgt agagtattaa ataaaaataa cttttaatta ttgtatacag
    17161 gaggagttgt tgtggataac atagaattgt taaggttggc agaagatgcc agacaacatt
    17221 cttatgctcc ttactcaggt tttagagtag gtgcagctct tttaactaag agtggtaaag
    17281 tttatactgg atgtaatata gaatgtgcat cattaggtgg aacaaattgt gcagaaagaa
    17341 cagctatttt taaagcaata tcagagggtg ataaggatat ctataaaata gctatagcaa
    17401 gtgataactc agaaaataat gagcaaacat atccatgtgg aatatgcagg caggtaatta
    17461 tagagtttgg ttctgatata aaaataataa cgggatatac taaaggagag gtgtttgagc
    17521 atagtataaa ggatttgttg ccaaactatt tctctggaaa tgatttaaaa taaatttaaa
    17581 agtataaata aatttaagta attaaaacaa gaagataaaa agttaggaga cagttaatgt
    17641 tcaaatcagg ttttgtcagt atagtgggta gaccaaatgt aggaaaatct actttaatga
    17701 ataatgtagt tggagaaaaa atagctataa tgagtgataa gcctcaaact acaagaaata
    17761 caatacaagc agtatataca gatgaagaaa tgcagatagt attcttagat acaccaggta
    17821 ttcacaaacc aaaaaataaa ttaggtgaat ttatggtaaa ggcagcaaca gaagctttta
    17881 aaaatgtaga tttaatatta tttgtagttg atgattctaa aaaaataggt cctggagata
    17941 gaaagataat agaggattta agaagtgtta agacaccaat aattttagtt gtaaacaaaa
    18001 tagaccaact agaccaaaaa gatgagctat ttgatataat caaaatgtat gatagagaag
    18061 gcatttttaa agaaatagta ccaatatcag cattaaaagg aaagaatacg gataccttaa
    18121 taaaagttat acaaaattat ttagaagaag ggccaaagta ttttccagat tatatgatta
    18181 cagaccagcc agaaagagta ttaatagcag aacttataag agaaaaagtt cttcactatc
    18241 ttaatgatga aatacctcat ggtgtagctg ttgaaattga aaaaatgaaa gcgagaaatg
    18301 ataaggaaat agtagatgta tctgctgtaa tatactgtga aagagattca cataagggaa
    18361 taataatagg aaaaaatggt aggaaactaa aaggaatagg taagtcagca agacaagata
    18421 tagaattgct tttaggttca caaataaatc ttcaattatg ggttaaagtc aaagaaaatt
    18481 ggagaaattt acaaaattat ataaataatt ttggatataa tgataaataa aggtgggtta
    18541 tatggatagg aaactggatg cttcccaaga tttattatat gaagtaaaat cattaattga
    18601 taataataaa gtattggagt taagagaatt aatagaggaa tatcatataa ttgatatatt
    18661 cgatataatg gaaaatttag aggaagacat gaaaattcaa ttatttgaag tacttccttt
    18721 agatatggca tcttctatat tagaagaagg tagtgtagag ttttttatta gtattttatc
    18781 taaactggat gtagaacact cgaaaaatat acttgaactt atgtctcttg gagatatggc
    18841 agacaagttg agtgaacttg aagaagaaga aagagaacac ataataaatc ttttgaatca
    18901 agagaatgcc gattatgtaa aagagttact attctatgat gaagattctg ctggtggaac
    18961 aatgaccact ggatatattt ctataaataa agatatgaca gctcttgagg ctatagacca
    19021 tatgagagaa gaagctgaag aagctgaaac tatttattat atatatgtgg ttgatgatga
    19081 agaaaaacta gttggagtat tgtcattaag agaattaata atagctagag acgcaaatat
    19141 agtagaagat ttgatgagtg aaaatataat ctctgtttat gtagatgaag atagagaaga
    19201 agcagtaaga ttagtatcta aatacaattt aatagcaatt ccagtagtag acagacaaga
    19261 aaaattaaaa ggaataataa cagtagatga tataattgat gtaatggaag aagaagcaac
    19321 tgaagatatg tataaatttg caggaagttc agaacatgaa agagaagttg ctgaaaaaga
    19381 aaaccctaca ttgagagagc aaataatatc agcacttaga ggacgattac cttggttaat
    19441 tattacctta gttggagggt tgttggcttc gttaatactt tctaacttag actatataat
    19501 gaatcctgta tatgctccat tagtattttt tatacctgta gtaattggta tgggtggaaa
    19561 tattggaact caatcatcat ctgttacagt aataacatta tctaataagg atttaaattt
    19621 tagcaatgtg gttagagaag gtattgtagg tattattaca ggtcttttgt gtagtataat
    19681 aacaggtatt gtaatttact ttgttatgag aaagttagac attgtattaa ttgtttcaat
    19741 atcattattt ataaatatgg ttttaggtgc aacaataggt gcgtttatgc ctgttttatt
    19801 aaaaaaaatg gatgcagacc catctacagt ttcatcacca attatatcta cagctcttga
    19861 tataactggt atagcagttt actttataat aacaacggca ttattgtcaa aaattgttta
    19921 ataactttat gtatgattta tcctcttctt actaaaaata taagtataaa tgtaagaagg
    19981 gggtttttta aaaatgaaca atatcagttg ggaagttttt aaaaaaacag gtagtataga
    20041 tgcatattta tatttctgtg attgcaaaaa catacatgaa gaaattgaag aagtaaggga
    20101 gaaaaaagaa gacgatgata atcctgaaca cccaggggat agtacttaaa gctataaggt
    20161 ataaggaaag tgatataata cttacactat ttacaagaaa gcttggaaaa gtatctgcaa
    20221 tagcaaaagg agctaaaaaa aataagagct ctttgctttc atcatcacag ttattttctt
    20281 atagcaattt taccttaaaa aaacaaggta atatgtacaa ggttactcaa agtgaaataa
    20341 taaaaagttt ttataatata tcctatgata tagaggcatt ttcttatgct acatatatta
    20401 caaaattagt tgaaaattct atacttgaaa atcagactaa taataggcta tttatattac
    20461 ttgcgcagac tctgtatttg tatacacagg ataatacaga caataggttt ataacagcag
    20521 cattcgagtt aaaattttta gactatatag gatttaaacc tatagtaaat aaatgcatta
    20581 attgtgaatg taaaatttta caaaattctg tgtttaatat ctacgaaggt ggtatactat
    20641 gtagtaagtg tagtaaactt tttgataata atataaaatt agacttaaca actataagtc
    20701 ttatggaata tgtattaaga aatgatattt taacatgtag taaagctaaa gtatcaaaat
    20761 atataacaca tgaacttgag aatattctag acaagtattt gaaagtttat gttgataata
    20821 ttaattttaa atcattacac gtattgcaaa gtgtaaaaaa taataaggga gtggataatg
    20881 atgagtagta atataactat tgaaatggtt gattcagtct ttgaaagagt tccaggagca
    20941 acttatgcag aagcaaaaga agcattagta atgtgcaatg gagatgtaat tgaggcagta
    21001 atatatttag aaaataaaaa aaatacttgt aattgtaaag caaaatctgc taaagaaact
    21061 atggaagaag tatttggcaa agatggagaa aatataaagt ttcaattaaa agagcttctt
    21121 agaaaaagta gtgtagtgag aataatagtt gaaaaagatg gaaaaactat gatgaatatt
    21181 cctttaacag ttggagttgt tggattagct tttgtaccat tagctacttt agttggatta
    21241 tcagctgctg ttataagtaa atatcgtata aaagttcaaa atgaagaaga tggagaaatt
    21301 gtagatttag gtgaattgaa tgaagaaaaa cttcacgttt tgaagaatat gataactaat
    21361 gcagcaaaag atgtgaagga tgtagtagtt gacaaaaaag aagatgacaa agatgtaact
    21421 gctgacctta tgaaagaaga atctgaaaag aataaagaag acgaagacga aaataaatag
    21481 atataaagtg acttttaaaa aatgaagaga agataattat aagaattatc actaacattg
    21541 acaaatttta tagaatttga tattctaaaa gttgtagaag aactattaaa atagcctttc
    21601 gtgtagacga gaggctttat tgttatagga gggattttta tgaattttca agagatgata
    21661 ctggcgttgc aaaaatactg gtcaaagcaa ggttgtatca tgatgcaacc ttatgatatt
    21721 gaaaaaggtg cgggaactat gaatcctaat acttttttaa gatcattagg acctgagcct
    21781 tggcaagtat gctatgtaga gccttcaaga agaccagcag atggaagata tggtgaaaac
    21841 ccaaacagac tatatcaaca tcatcaattc caagttatac taaaaccttc tccagataat
    21901 atacaagaat tatatttgga aagtttgaaa gagattggaa ttgacccaag tgagcatgac
    21961 ataagatttg ttgaagataa ctgggaagct gctacagttg gtgcttgggg acttggatgg
    22021 gaagtttggc tagatggtat ggaaataact caatttacgt attttcaaca agtaggtaat
    22081 attgaatgtg agcttgagac aggagaaatt acatatggat tagaaagatt agctatgtac
    22141 attcaagaag ttgatagtgt ttatgattta aaatggaatg ataaaataac ttatggtgaa
    22201 gtatttaata aagctgagta tgaaaactca atgtacgcat ttgaattatg tgatgcagat
    22261 atgttgttta atctgtttga tatatatgaa aaagaagcat taagacttat ggaaaatggc
    22321 cttgtaatac cgtcatatga ctatgtgtta aaatgctctc atgcatttaa tacattggat
    22381 gcaagaggtg caataggagt tagtcaaaga gcttcattta taggtagagt tagaaatatg
    22441 gcaaagactg ttgcagaaac ttttgtaaaa caaagagaag aaatgggatt ccctctttta
    22501 aaggacggtg acaaataatg aaaaattatc ttttatttga aatcggtgtt gaagaaatgc
    22561 catcaagatt tgtaggcagt acactagaac aattaaaaaa caatcttagt aaattatttg
    22621 atgaaaatag aatagctttt gataaaataa atgcttatgg gacaccaaga agacttactt
    22681 tagtagtcga aggattgagt gatagacaaa gtgatttaga agaggaaata aagggtcctg
    22741 ctaaaaaaat tgctgttgat gcagaaaata atttaacaaa accagcatta ggatttataa
    22801 aaagcaaagg attaagcgaa tctgacatat attttaaaca agttggaaaa gaagaatata
    22861 tatttgcgac tataaagcaa gatggaaaag aaacttcaga agtactaaaa gacatattac
    22921 cagagactat aaaatctgta gtattcccaa aagctatgcg ttggggagga aaaaatctta
    22981 aatttgcaag acctataaga tggatagttg cattgttaaa tgataaagta ttagagtttg
    23041 atttagaagg aataatatct tctaatgtaa caaaaggtca tagattttta ggtgaaagta
    23101 caatagaagt tgattctata gaagattatt ttgaaaaatt agaaaaaaat tatatagttt
    23161 tagaccaaaa caagagaaaa gaaatgataa gaaaacaatg tatagaagta gcaaattctt
    23221 taggtggaga agttgaattt gatgaagatt tacttgaaga ggtaacttat ttagttgaat
    23281 atccaactgc tttttatgga gaatttgatg tggattatat aaaactacca aaagaagttg
    23341 ttataactcc tatgaaacaa catcaaagat attttccagt acttaaagaa ggaaaactgt
    23401 tacctaactt tatagctgtt agaaatggtg attcttacag aatagacaat gttaaagctg
    23461 gaaatgaaaa agttttagaa gcaagacttg cagatgcttt atttttctat agagaagata
    23521 ctaagagaaa cttagaaagc tacatagaaa aattaaaaac tgttgtattc caagtaaaat
    23581 taggaactat ttacgacaaa actttaagac ttgaaaaatt aagtgctgat atacttgata
    23641 gtttaggttt agtagatgaa aaggcagata ctataagggc tgctaaatta tctaaggcag
    23701 atttagttac tggaatggta ggagaatttg atgaattaca aggttttatg ggaaaagaat
    23761 acgcaaaagt tggaggagaa aatgatgttg taagtgaagc tatttttgaa cattatcttc
    23821 caagatttgc aggagatata cttcctaaga ctaatgcagg ggttgcctta tcaatagctg
    23881 ataagttgga ttcaatagca ggtttctttg ctataggaat acaaccaaca ggttctcaag
    23941 acccatatgc tttaagaaga caagctttag gtatacttag tatacttatg gacagaaaag
    24001 ttgctgtgga tataaaaata cttattgaac atgctttaga caattattct gaattagatt
    24061 ttaataaaga agaagttgtt gagcaaataa tgaatttctt caatgagaga gtaaaaaatc
    24121 tatttaaaga tttaggaata agatatgatg ttgttgatgc tgttttaagt tctgatataa
    24181 atgatgtttc tgatatgcat atgagagctt tagagcttaa taattggcta gaaaaagatg
    24241 aattagtgga gatgcttact gcatttaata gagtttctac tctagctcaa aaagcagaaa
    24301 gtgataaaat tgatgaatct ttattaaaag aagatgctga aattaaactt tacaatgaat
    24361 ttaaacaagt aaaggtaaaa gttaatgaat tattaaagga taagaaatat ggtattgctt
    24421 tagatgaatt tgcatcttta agaccagtag ttgataattt atttgataca gttatggtca
    24481 tggataatga tgaagctatt aagaacaata gacttgcttt acttaaacaa gtatatgata
    24541 ctatgctagg aatttgtgat ttatctaaaa ttgtttataa ataaaattta aactatttat
    24601 aagaaataag gctgtctcaa aatagagata gtcttatttt tcattgttga gatgatgaaa
    24661 atatatttga tttcatagaa aaagagtgtc tcataaacta aaaagttcat tttgagacac
    24721 ccatgttttt tgtaagtaaa tataataaat tatattttat acttatcagt tattttgttt
    24781 aaaatattat catttgaatt tacccattca taaaaattgc ctgaagatgc gtccgatatt
    24841 ttataaagca tttcaggaat atcactagct ttaaaatctc caagtgattt tcccaatcta
    24901 gtcttaccta tttcaaaact gcctccaaca aataattcaa atgcatctgt agatatacca
    24961 tcaacattct tctttttacc acataaacct atgctaccaa cttgatgaac accacatgaa
    25021 tttaaacatc ctgatatata taacttaggt gctttattta aaatttcttc attattttgt
    25081 aatctaaaat aatctatgat ttcatgtaac attttctgac tattttgtat gcccatttga
    25141 caaattggaa cacctatgca agatatactt tgttctaatt gagaattaca actaatagtt
    25201 ttacttattt ctaatattct ttttgcttca tttccattta aatttaaaat atataatccc
    25261 tctgtcattc ctagtctaat cataggattt tcaacattat ctaattcttt aagtaaagta
    25321 cttaaatcct ttgttaacaa aatacctcca actggatgta tatatacagt gtataaacca
    25381 tcctgttttt gaggtattag tctagaatct tgtatatcta ttttaactcc aggtttagag
    25441 taatctattt cttcaatatt gagttcaaga ctaccatttt ctttttctaa tttatagtat
    25501 tctttaaatt tttctaagaa tagttcttca ccaagtgatt cgaccatata tctaacccta
    25561 gctctgtttt tattttcata atttccataa tccataaaga atttaatcat accttcaaca
    25621 caatataagg cgtctttcgg ctttataagt tcatcaagtt ctaaagcaac tttgggttgt
    25681 ttacctagac ctccacctaa gaaaactctg aaatatggtt catcattttt caaagtagca
    25741 acaaatccta agtcttgtat actacaatga gcagtatctg tgtaacaatt agaataagaa
    25801 acttttaatt ttctaggtaa atggtatgta gtagcatttt tgataaaata tttatcagta
    25861 gcaactgcat atggagtaac atcaaagact tcactagggt caactccaga taatggagat
    25921 aatccgacat ttctaggata attaccacca ccacctctag tgaatatatc attttttatt
    25981 ccttcttcca tgatatcaac aattgaattt atagataaat cgtgtaattg tatagcttgt
    26041 ctagttgtta agtgtatctt atctaagttg tgtttaatag ctatttcata gattttatta
    26101 agttgatact gagataaaac tccagcagat actctaagtc ttatcataaa agattttttg
    26161 tctctttgag cataaacacc atatccacca gaaaatccct tatattgcat tttatttatt
    26221 tctccattta aaaattttga agaatattct ttaaaattag gtagttcatt taacaatatc
    26281 tttttaaaat tatccattat ttcacctcat tgttagtctt gtataaaaat atatctattg
    26341 tataatagat atatatatta ttatctagtc aggataaaaa tataacagaa taattctatt
    26401 aaaagtaaga ataataagga catagtgagt attttgtttt tattttacaa attaagtata
    26461 atatttttgt tttataaaat ataattaaga taaatagata attttgtaat tttaatatat
    26521 aatatttagt agagtgtggg agtagcactt aatatttgca ggaggagttt ttgtatatgg
    26581 gagaaataat aaatgcatta gatagagccc tagacataat tttgctgtta tatcatgaaa
    26641 agagagaaat gggaataact gaaataagta aggctatggg agtatataaa agtactgtac
    26701 atagaacttt ggttactctt gaaaacaaag gttttgttat acaaaatgca gagaatggta
    26761 agtattggct aggaataaat ttatatgcaa taggtatggt tgtgggagaa aaaatgtcat
    26821 tgactgaaat agtaaaacca tatacgaaaa aattaaatca agaatttaac gaagtagtta
    26881 atgtatctat attagaagaa agagctcaag attctccaag aagtatcata atacataaag
    26941 agtatggttc aaatcaatta ctttcagtaa atccatccgt tggttcaagt agtgaatgtt
    27001 attgttcagc agttggtaaa tgtcttatgg catttaatga ttctatagat tttgaaaaat
    27061 atagaaaaac accaatacac aagtatacag aacatacaat tgataattgg gatgatatga
    27121 tgcttttttt agcaaaaata aaagaacaag gatatgctat agatgatgaa gaactagaac
    27181 atggactaac ttgtattgga gcacctatct tagataaaaa taataaagct attgcagcca
    27241 taagtttgtc aggtccaact ataagaatga gagaagggga ctttgagtat aagattaaaa
    27301 gagttataga aactgcaaaa agtatatctg aactttttag atgattttta atctcgataa
    27361 taaaaatagg ctatatttaa attgttattt gtaaatatca gtttaaatgt agcctatttt
    27421 tctttaatct aaaattaaat attcttaaaa tttaaaatgt tattgaaata tataagcgaa
    27481 tgtgttatct tttaattaag caataaaaat agaacaaggt tctatactat agaacaaaat
    27541 tgatgcatat atagtattta tacaaaatat aaatattata tatttaaaat taagtaaaaa
    27601 tattacataa aggggagatt tgagaaatgg aaaaagtcaa gttgagtgtt gaaagatgta
    27661 agggatgcta tctttgtatt gaagcttgca aaaaagatgc aataagtctt tctgactaca
    27721 caaataaaaa aggctttgta actattaaag tcgatgaaga aaaatgtatt caatgtggcg
    27781 cttgttatac aatgtgccct gatttagtat ttgagatatt ataaattatt tttattagag
    27841 gggagaaaag gtatgcaaag tttattaatt aaaggaaatg aagctttagc agaaggtgct
    27901 atacgtggtg gttgcagatt ttatgcaggt tatccaataa cgccacaatc agaaatatta
    27961 gaaatgatgg catggagaca aaaagaagtt ggaggagtat ttatccaagg tgaatcagaa
    28021 atagccagtg taaatatggt tatgggagca agtgcacttg gagcaagagc tatgacttct
    28081 tcatctggtc caggattttc attaaaacaa gaaggtattg catattgggt atcagctggt
    28141 ttgccagcag ttgtagcatg tgtgcaaaga tatggtatag gtgatggatt tataggagct
    28201 ggtcaagata attattggca agcagtaaaa ggcggtggaa atggagatta tcatttaatc
    28261 gtgttagcac ctaatagtgt tcaagaaaat atggatatgg catatgaatc ttttgaattg
    28321 gctgaaaaat atatgcatcc agtacttata ctttctgatg ctacaatagg tcaaatggta
    28381 gaaccttgta tagcaccacc aatgaaagaa tacgatatgg ataaagctcc atgggctgca
    28441 aaaggaacac ctgtaggaga aaaaaataaa gtaataactg atataacata ttttgaagac
    28501 caagatacat ggcaaaaagg gtatatagaa aaaatgagaa aagttcaaga agatgaacaa
    28561 agatgggaag agatagaaat agaagatgct gaagtggtat ttgtggcata cggtatttca
    28621 tcaagaatag cagaaggtgc agttagagat gctagagcaa aaggaatgaa attaggtcta
    28681 ataagaccaa aaacattgtg gccttaccca agaaaagcat ttaagaaatt aagtgattca
    28741 ttgaagggac ttgtaacagt tgaaatgaac atgatggcac agatgaagga agatgtaatt
    28801 atagcatgtg ataatagatt tccagtatat tcagtagcaa cagctcaata tatggcagaa
    28861 gtaaatcaat taatagattt tgctcaacag attatagatg gaaaagctaa gcaagaggag
    28921 gtattttaat tatggctaaa aaagcaccag caataatgat gtgtgaaaat atgttctgtg
    28981 gaggctgtgg acactctata gtcgatagaa ttattggaga agtattaaca gaaatggata
    29041 tatgtcaaga tacaattata tgttcagata taggttgtaa tcatatgttt caatttagta
    29101 tgaatgtaga tgttgtcgtt ccacctcatg gtaaaatggg agcagcaatg gcagctatga
    29161 aaagagttag accagataaa tatatattta caatagctgg agatggtgga gcttatgcaa
    29221 tagggcttgg agaaactatg tcagctgcta ctagaaatga taatgtaata tttcttgtta
    29281 tgaacaataa tatgtttgca atgacaggag ggcaaatggc tccaactact atagaaggtc
    29341 aatatacagc ttctactcca gcgggtagaa attacgcaga tcatggttca acatttgatg
    29401 ttgtaaaagt tatgggaaat ttagatatag catatctagc aagaggaaca ataactaacc
    29461 ctaaagaaat aaatacaaca aaaagataca tcaaaaaagc tctagaaaaa caaaaagaaa
    29521 agaaaggttt ttgcttaata gaagtgcttg taccatgtcc aacaaactgg ggattaaagc
    29581 cagtagatgc tatgaagaga attgaaaatg aacttgttcc aaagtatcca cttggagaat
    29641 ttattgatta gggaggaaat aatatgttga atgaaattat atgtgctggt tttggaggac
    29701 agggagttct tacagcagga aaaattataa tgtatgttgc atataaggca gaaaagaaaa
    29761 ttacatggtt cccatcttat ggaaatgaga tgaggggagg aagtgcaaat tgcaatgttg
    29821 ttgtaagtga tgaaaaaatt gcaaatcctt gtgtaaaaaa tccagatata ctattgggaa
    29881 tgaacagcgt atcagtagat aagtatgaaa gtttaatgaa aaaaaatggt tatatgtttg
    29941 tcaatgaatc actagtaagt aaggatagaa agtacagaga agacattaat gttataaaag
    30001 tacctgttac tgaaatagca caaaatttag gcaatgaaaa agctgctaac atttgcatgt
    30061 taggtgctat ggtaaagagt ttggatttat ttacaaaaga agaatttgaa agtggtatgt
    30121 gtgaatactt tgaaaatcaa ggcaaaggta aattcaattc taaaaatgta gaagcatttg
    30181 aagctggata cgactacgaa tgaatatttt aaatatttaa aataattatt gaaatactta
    30241 tttttatatg ttagtattta attaataaaa tatagaacgt cgttctgaac aataggacga
    30301 agtctttagg tcggaataca agaacgacgt tctgtatagt agaactaggg ggtgttaaat
    30361 tgaaagtttt agtaataaat cctggtggta catccacaaa aatttctgtt ttccaagatg
    30421 aaaatgaaat tttgaaaaaa aatataaccc atacaagaga agatttaaaa aatttttcta
    30481 aagtatttga tgaatatgat tatagaaaac aactgatagt agatatttta agtagtgaga
    30541 atcatagtat aaattctttt gacgcagtag ttggtcgtgg tggacttatg aaggctataa
    30601 aaggtggtac ctatactgtg agtgaagaaa tgattgagga tatgagaaat gaaataaatg
    30661 gggaacatgc atcaaattta ggtgcattat tagctaaaac tatagctgat gaaataggag
    30721 tacaatcatt tgtagtagac ccagtttctg tagatgaatt tgatgatgta tcgaggatta
    30781 caggaatatc agacatagaa aaagcaagtt ggttacatgc attaaaccat aaagctgttt
    30841 caagaaaaat tgcacaaaaa ctagggggaa aatacgagga ttataacttt attgtagctc
    30901 atttaggttc aggaatatcg attgtagcac ataaacaagg caaaatgatt gatggaagtg
    30961 gtggtaggtc tgatggtcca ttttctccag aaaggagtgg tggattactt gcatatcctc
    31021 ttatagaatt gtgttactcc ggaaaatata ctcatgatga aatggtcgca aaagtaagtt
    31081 cgattggggg aatgtacgat tatttaggaa caaaggatat gatagaaatt gaaaacagaa
    31141 tcaaggaggg tgatgaaaag gcaaaactta taatggatgc atttatttat caagtagcaa
    31201 aagaaatttc tatgtatgga gcttcacttt cagggaatgt agatagagtt atcttgacag
    31261 gagggatatc tcactcagaa atggtagtca aaggagtagc agaaaaagtt tcatatctag
    31321 ctccaataga gatagtacca ggagagatgg aaatggaggc acttgctcta ggggcactta
    31381 gagtacttaa tggagaagaa atagcaaaaa cttattaata tttaggagaa agagtatgtt
    31441 aaaagatttt aataatttat tagaagaggc taaaaagctt gaacctgtta ttgtaagtgt
    31501 tgctgttgca gaagacaaag aagtattaaa ggctgtgaaa tcagcaacag atttaaacat
    31561 tataaagcca attcttgtag gagacaagga taaaatagaa agtatatcaa gagaaattgg
    31621 attaaaaggt catgaaataa taggttgtca aaatcccgat gaatcagtta aaaaagctgt
    31681 agagatagtt aaaaataagg aagctaaagt tcttatgaag ggattagtaa atacaagtgt
    31741 ctatatgaga gctatattaa ataaagaaac gggtcttaga acaggaagat tattaagttt
    31801 gcttgcagta tatgaattac ctcaatatca taaacttctt tattgtactg atagtggaat
    31861 aaatgtatcg ccaaacttaa atcaaaaaaa agatataatg acaaatgcac ttcttactat
    31921 gaaaagtata ggcatggaaa atcctaaaac tgcgatactt actgcaaatg aaatggtaga
    31981 ccctaagata caatctacag tggatgccaa cctacttgta gagatggaaa aatcgggaga
    32041 aataccaaag tgtatagtgg aaggtccaat agcatttgat gtagcatttg atgcacatgc
    32101 agcagaacat aaaggtatta aaagtaatat tgctggtgat gttgatttat tagtatttcc
    32161 aaatatagaa gcagggaata tattaggaaa gtcatggctt agatttaata aagctaagtg
    32221 ggcaggtata gttttaggag caacaaatcc gataatttta ggttcacgtt ctgacacagc
    32281 tgaaataaaa ataaattcaa tagcattagc gtgcctagca tcacaataag aaattattat
    32341 taaggaggaa aaagtattat gaaatatgca aaaagaatgg atatggtaaa agcttcagct
    32401 ataagagatt ctcaaaagaa aatagctcag aaggtagcaa gtggtggaac tgttatatct
    32461 tttgctgcag ggcttcctga cccaaattta tttccaatag aagagataag tgaaataact
    32521 caaaaagtac ttaatgaaag gggaaaattt gccctagcat atggacctac taaaggtgaa
    32581 gatgagttgt tagatctatt agtaaaaaga atgaaagaag aagaaaatat tgatacaaaa
    32641 gcagaaaata ttataataac tactgggtca caacaaggga tagcattaag tgctatgatt
    32701 ttattagaaa agggagatgt agtagttaca gaaaatccaa gttacttagg tgcaataaat
    32761 gcatttagac cttatgaatg tgatttttta ggagtagata cagatgaaga aggaatggta
    32821 acagatgatt tagacaaatt attatctgaa aattctaaca taaaggtgat atatgtaata
    32881 ccaacatttc aaaaccctac aggaaaaaca tggtctttac aaagaagaaa agattttatg
    32941 gaagtcgtga ataaatatga tgtcatagta atagaggata atccttatgg agaaataaga
    33001 tttacaccag aacctctacc tacacttaaa tctcttgata ctaaagataa agtattgtac
    33061 cttgggtcat tttcaaaggt attatgtcca ggatttagag ttgcttggat gtgtggtaat
    33121 gtagacatca tagataaggc agaactttta aaacaaggta tagatttaca gtcaaatcaa
    33181 ttttcgcaag tacaagtaat agaattttta aagaaataca acttaaatga acatatagaa
    33241 aaaataagag cagagtataa gaaaagatgt ctgctaatgt tagatgctat gaagaaacat
    33301 ttcccagagg aagctaaata tacagaacca gatggaggaa tgtttatatg ggttgaactt
    33361 cctgaaagta taaatgtaga tgatttatta gataaagcca ttgatgctgg agttgcttat
    33421 gtttctggag agtccttctt tgcaaataat ggtccaaaaa atactatgag acttaactat
    33481 acaacaatgt cagaagaaca aatcgttaaa ggtgttgaga tacttgcaga agttataaaa
    33541 aaagaactca attatgcttg tgaataaata taaaaagtga gggtttaaat atgggagaaa
    33601 aagaaaacgg tataaagttt ggaaagttaa tgtttcaact tgttattata gcaatatctt
    33661 gggaattagt ttatattata ccatttatcc aatatacatt atatgatcca attctaaaag
    33721 cattagaatg tacaaacact caactaggtt ttctacttac aatttatggg ttaggtaata
    33781 tatttggagc tcctatagga ggctggttag cagatagatt tgattataga aagataattt
    33841 taggttctgt atttcttaat ggagtagtat catttttatt tgcatttaat atgaactatc
    33901 catttgcagt tgctacttgg gtaggttgtg caatcacatc attagttatg aactatcctt
    33961 ctatggtaaa aatacttaga gttataggaa aagataatca aggtaaggtt tatggattta
    34021 atgaagctat ggttggagtt tctggagtag taatgggagc aatattctta tatatttata
    34081 catgctttgc aactcctaca ttgggtatga gatgggtaat gatatcttta ggaatattat
    34141 caatagcaat gtgtccagta ttatggtttg taattaaaga tgtagatatt aaagaagaaa
    34201 gagaagaagc aggtgaaaaa atgtctgctg gtgattttat aacagtacta aaatcaccaa
    34261 atacttggct agtaggaata agtatattct gtgtatattc attcacagtt acaatgtctt
    34321 attttacacc atatataaca tctgtacttg gaggttctgt agcattatct ggtgcattag
    34381 caataataag acagcatggt ctaaaattat ttggagcacc atttggagga tattgtgcag
    34441 acaaattaaa atctcctaca aaagtacttt taccaatata tgtatttggt atagcagtaa
    34501 tagttttatt cctagtatta cctgcatcaa ctccaatgac tatattcata gctttaacat
    34561 ttgtagtagg tatattaggt tacatgggaa aaggtatata ttatgcagta caagatgaag
    34621 taaaagttcc agttaaatat tcagcaacta ctataggtat agctgcagct ttaggatttt
    34681 caccagatgt attccaattt gcattaatag gacactggat agatacttat ggcaataaag
    34741 gttacacata tacatttata ttccaaatag caattctagt tattggaata ctatcttgtt
    34801 tatatatatt aaaggttaaa aagagaagag caagtaaatt agaagcttaa atattcaatt
    34861 tggtaattaa gtatatataa atacaatact tattttgatg aatgtgattg tataaataac
    34921 ataaaaaata tcaatagatt aagttttata ataatgttta gagaataaag tataaaaatc
    34981 aaaatgttat caagttttct aggttttaac ataaagttac ccctgaaggt gtaaaattga
    35041 gtggcaatta ttatctattt tcttacctac ataaacctat ttaatttaaa ttctaagaga
    35101 ttttaaaaat agatggtagt tgtactcaat tttaaacctc ataataaata cga
//
LOCUS       DBGJFU010000029        34624 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.029, whole genome shotgun sequence.
ACCESSION   DBGJFU010000029 DBGJFU010000000
VERSION     DBGJFU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.029"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(483..2870)
                     /locus_tag="ACOIAS_001374"
     CDS             complement(483..2870)
                     /locus_tag="ACOIAS_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGZ2701090.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTDPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2893..3258)
                     /locus_tag="ACOIAS_001375"
     CDS             complement(2893..3258)
                     /locus_tag="ACOIAS_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGZ2701091.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3457..4305
                     /locus_tag="ACOIAS_001376"
     CDS             3457..4305
                     /locus_tag="ACOIAS_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701092.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4742..5830)
                     /locus_tag="ACOIAS_001377"
     CDS             complement(4742..5830)
                     /locus_tag="ACOIAS_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701093.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5955..6683
                     /locus_tag="ACOIAS_001378"
     CDS             5955..6683
                     /locus_tag="ACOIAS_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2701094.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6885..7376)
                     /locus_tag="ACOIAS_001379"
     CDS             complement(6885..7376)
                     /locus_tag="ACOIAS_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701095.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7387..7830)
                     /locus_tag="ACOIAS_001380"
     CDS             complement(7387..7830)
                     /locus_tag="ACOIAS_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701096.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7820..8659)
                     /locus_tag="ACOIAS_001381"
     CDS             complement(7820..8659)
                     /locus_tag="ACOIAS_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HGZ2701097.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8878..10263)
                     /locus_tag="ACOIAS_001382"
     CDS             complement(8878..10263)
                     /locus_tag="ACOIAS_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2701098.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10436..11179)
                     /locus_tag="ACOIAS_001383"
     CDS             complement(10436..11179)
                     /locus_tag="ACOIAS_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HGZ2701099.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11199..12461)
                     /locus_tag="ACOIAS_001384"
     CDS             complement(11199..12461)
                     /locus_tag="ACOIAS_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2701100.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12645..13643)
                     /locus_tag="ACOIAS_001385"
     CDS             complement(12645..13643)
                     /locus_tag="ACOIAS_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701101.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13627..15138)
                     /locus_tag="ACOIAS_001386"
     CDS             complement(13627..15138)
                     /locus_tag="ACOIAS_001386"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701102.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15192..16154)
                     /locus_tag="ACOIAS_001387"
     CDS             complement(15192..16154)
                     /locus_tag="ACOIAS_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGZ2701103.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16202..17092)
                     /gene="rbsK"
                     /locus_tag="ACOIAS_001388"
     CDS             complement(16202..17092)
                     /gene="rbsK"
                     /locus_tag="ACOIAS_001388"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGZ2701104.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17134..18156)
                     /locus_tag="ACOIAS_001389"
     CDS             complement(17134..18156)
                     /locus_tag="ACOIAS_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2701105.1"
                     /translation="MNFLSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKI
                     MKEKNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNT
                     DEDAKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETL
                     KGIVKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYN
                     PAYVFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVV
                     GFDDIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRD
                     STKESE"
     gene            complement(18375..19352)
                     /gene="bioB"
                     /locus_tag="ACOIAS_001390"
     CDS             complement(18375..19352)
                     /gene="bioB"
                     /locus_tag="ACOIAS_001390"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGZ2701106.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19558..19821)
                     /locus_tag="ACOIAS_001391"
     CDS             complement(19558..19821)
                     /locus_tag="ACOIAS_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701107.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19891..20964)
                     /locus_tag="ACOIAS_001392"
     CDS             complement(19891..20964)
                     /locus_tag="ACOIAS_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HGZ2701108.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21223..21981)
                     /locus_tag="ACOIAS_001393"
     CDS             complement(21223..21981)
                     /locus_tag="ACOIAS_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGZ2701109.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(21978..22898)
                     /locus_tag="ACOIAS_001394"
     CDS             complement(21978..22898)
                     /locus_tag="ACOIAS_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2701110.1"
                     /translation="MMNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKT
                     TTMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCG
                     EITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDE
                     PTSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLS
                     EIKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTV
                     ISTLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22895..23095)
                     /locus_tag="ACOIAS_001395"
     CDS             complement(22895..23095)
                     /locus_tag="ACOIAS_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HGZ2701111.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23100..24194)
                     /locus_tag="ACOIAS_001396"
     CDS             complement(23100..24194)
                     /locus_tag="ACOIAS_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2701112.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24704..24844)
                     /locus_tag="ACOIAS_001397"
     CDS             complement(24704..24844)
                     /locus_tag="ACOIAS_001397"
                     /inference="COORDINATES: protein motif:HMM:NF021468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HGZ2701113.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(25154..26461)
                     /locus_tag="ACOIAS_001398"
     CDS             complement(25154..26461)
                     /locus_tag="ACOIAS_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2701114.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIVHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26526..27005)
                     /locus_tag="ACOIAS_001399"
     CDS             complement(26526..27005)
                     /locus_tag="ACOIAS_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HGZ2701115.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27052..27915)
                     /locus_tag="ACOIAS_001400"
     CDS             complement(27052..27915)
                     /locus_tag="ACOIAS_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2701116.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27915..28670)
                     /locus_tag="ACOIAS_001401"
     CDS             complement(27915..28670)
                     /locus_tag="ACOIAS_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2701117.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28697..29185)
                     /locus_tag="ACOIAS_001402"
     CDS             complement(28697..29185)
                     /locus_tag="ACOIAS_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGZ2701118.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29203..29604)
                     /locus_tag="ACOIAS_001403"
     CDS             complement(29203..29604)
                     /locus_tag="ACOIAS_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2701119.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29607..30104)
                     /locus_tag="ACOIAS_001404"
     CDS             complement(29607..30104)
                     /locus_tag="ACOIAS_001404"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2701120.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30128..30532)
                     /locus_tag="ACOIAS_001405"
     CDS             complement(30128..30532)
                     /locus_tag="ACOIAS_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2701121.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30762..33329)
                     /locus_tag="ACOIAS_001406"
     CDS             complement(30762..33329)
                     /locus_tag="ACOIAS_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2701122.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33495..34238
                     /locus_tag="ACOIAS_001407"
     CDS             33495..34238
                     /locus_tag="ACOIAS_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HGZ2701123.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT        11211 a         6349 c         3553 g        13511 t
ORIGIN      
        1 ttatgtttgc ccttttctta aggacaagta atactatatc accttaaaaa taattcgtca
       61 atacttttta aaattatttt ttaaaaatat ttactttctt atattacata atttattgaa
      121 tggcagtcat actgttggtt tagagtattt taacacacac attttgttgt aaaaattaca
      181 actattaata taatatatat acaatcataa gtattataat ttttatgaat ctaatttaaa
      241 cttaatataa acttattaat aaaccttcta tgtctaaatt ttaaaattac aatataaaag
      301 ctctacacta aataaataca ttaaataaac ataatggata atgaatataa atttatatat
      361 gcaaagcgct ccaataaaaa ggtaccaata aagttaaaag aaacttattg atacctttca
      421 ttattacata cttcaatata taattataag ttttttaaat agtatggatt tacatttatc
      481 ccttatctca tagctctcat agcatttact actgctataa gtgccacacc tacatcagca
      541 aatatagctt cccacatagt agctactcct ccagcaccta gtatcataac tataattttt
      601 actcctagag caaatattat gttttgccac actatcttat tagtcttgtt tgctatttca
      661 attgctttag atatcttgct aggctcatct gtcataagca ctacatctgc agcttcaata
      721 gcagcatctg aacctaagcc tcccatagct ataccaacat cagcacgagc taatactgga
      781 gcatcattta ttccatcacc tacaaacgct attttctctt tttcagttct tccttcatat
      841 aaatcttcta atctatctac cttttcatta ggaagtaaat ttgagtaaac tgtatccaat
      901 tctaattctt gagcaatatt ttttgctact ttctcattat ctccagttaa cattacaact
      961 tcttttaccc ctatttcttt taagcttctt atggcatttt tactatcttc ttttacttca
     1021 tcagagataa ctatataacc tctatatact ttatcaacag caatatagac tactgtgcct
     1081 acttctttag ctgatgaata agatatattt tcttttttca taagtttctc attacctgct
     1141 aatataaaat tgccattatg ctttactcta attccataag ctgctatttc ctcataactg
     1201 tctattgtat ctaaatcaat ttttttatta tagtaactta atatggactt tgctattgga
     1261 tgatttgaat ttacctccgc aatagctgca tattctaata attcttcttc tgatatacct
     1321 tcaggattta acttagttac attgaacaca cctttagtaa gtgttcctgt tttatcaaat
     1381 actactgtat ctacacttct taaagcttct aaatagttac ttcctttaat aaggatacca
     1441 tttttagatg caaacccaat tccactaaaa aaactaagtg gtattgataa aactaatgca
     1501 caagggcaag aaacgactaa gaatatcaat cctctatata accaatcact aaatacctca
     1561 cctgagatta caagtggtgg cacaaatgct attaataaag cagctattac tactattgga
     1621 gtatagtatc ttgaaaactt agatataaag ttttctgtct ttgatttttt aatgctagaa
     1681 ttttcaacaa ggtcaagaat cttagaaact gttgattctc caaaactctt tgtaacttca
     1741 atactcaata aagcattttt atttataaca cctgataaga tttcatctcc tttttcaact
     1801 tctctcagca ctgactctcc tgtgagtgca gaagtatcta acatagaaac tccatctact
     1861 actacaccat ctaatggtac tttttcacca ggttttacta ctattatatc acctacattt
     1921 acctcttctg gtgacaccac cttaacttca gaattaactt ttagatttgc atagtctggt
     1981 cttatttgca taagtgaagt tatagacttt ctggatttac caactgcaac accttgtaaa
     2041 tactctccta ctttatagaa aagcataacc cctactgctt ctgataactc ccctattgtt
     2101 aatgctccta ctgttgctag agccatcaaa aagttctcat caaatactct tccttttgat
     2161 atatttctta ttgctcttaa aagcacatca ccacctacta ttacataggc aactataaat
     2221 actatattgc taaactgact ttcataacct gtagctgttt gatatatacc aaatatatat
     2281 acaagtgctc caattataag tggaattaat tctttcttat ttgtatcatt tacagctcct
     2341 gatttttcag tagttctccc ttttactttg tcagtagacc ctacttgtat atcaagccct
     2401 ggctctgttg aatttattat ttcttttatt ttatttataa catcatcctc ttcaaagtta
     2461 ttacttatat ttactgtaag ttttttattt acaaagttta aattagaaga ttcaacttct
     2521 tttaatttag aaactttgtt attaatctct tctgcacaat gagcacagtt taatcctcct
     2581 aatataagct cttttttaat tgatgtttta ctagctgcat tttctttaga acttatttgt
     2641 atatcaagac ctggatctgt tgaatctatt atatctatta tcttttctat agtagtatcc
     2701 tcattaaaac tttcctttat atttactgta agttttttat ttataaaatt taaattggaa
     2761 gatttaactt cttgtagttt agaaacttta ttattaatct cctctgcaca gtgagcacaa
     2821 tttaatcctc ctagtataaa ttctttttta attgaattat ttgcttccat atatattcac
     2881 tcctctcatt tatcatttat aagtctctct aatatgattt aatccacaat caaatatctg
     2941 acttatatga ctatcatcta atgagtaata cactgttttt ccttctcttc taaactttac
     3001 taatctagcc tgctttaata ctctaagttg atgagatatt gcagaatgag tcatatttaa
     3061 taaactcgct atatcacata cacacatttc atttgcaaat aatgcatata atatctttac
     3121 tctagtagta tccccaaata ctttaaataa ttctgctaaa tcatataaca tttcttcatc
     3181 tggcatagtt gatttagcct ctgttactat ttcttcatgt actatattac aattacaatc
     3241 atttatctct tctctcattt ttaatcctcc attattttaa tatatgaata actgttcata
     3301 tgttcattat atgttatcat ttctaaattt gcaacaataa tttttattaa tttaatataa
     3361 atagaatgca catattataa acttttttaa tatatatatt attagaataa gtttttgtaa
     3421 acttttttat agttttctag gaggtgcaag tttaatatga gtaacaagaa aaaaaaggat
     3481 ttagatacta gttacatgcc ttacttagac ccagcgaact atgcagatta tattactaat
     3541 gaagaaattc cccaagatga attgagaaat gaccctcaaa tttctaatgg ttatacaaaa
     3601 atacctaata accagaacat aaataaccta tatcctaata atgtaaatca ttatcctaaa
     3661 agtagtaatt ctccaaacaa cttgcctaac acaaataata catccggaaa tatgaattac
     3721 aatacatctg ctacaaataa tatgcctaat aacatgaata ataatacggg tatgcctaat
     3781 aatatgtcta acaatatgaa tagtaatatg ggtatgccta acaatatgcc taataacatg
     3841 aatagtaata tgggtatgcc taacaatatg cctaataata tgaatagtaa catgggcatg
     3901 cctaacaatg tgcctaataa catgaatagt aatatgggta tgcctaacaa tatgtctaat
     3961 aatatgaata gtaatatggg catgcctaac aatatgtctg gcaatatgaa taacaatatg
     4021 tatggtaata caggtatgcc taataacatg aacagtaata tggggtataa tatgaacaat
     4081 actatgggta ttcctaactt ctcatgtaat caaaatatgc ctcctaatgt acttatgatg
     4141 ataccaggag ttatatgtca taatacaatg caaggtatgc ctgtagttat gcctagtact
     4201 atgcctccta atatatatcc tactccttat ggttctagta atatgtctgt acaaggtata
     4261 ccacaagcta ctaatattga ggaatttgat gaggaagaaa tgtaatatat atttttttaa
     4321 gtaagttgta taaaacatta ataattgacc ataataataa atgtattcct cataatattc
     4381 ccccaatatt attataggta tagaaagttg actgaatatg cgcttattcg gtcaacagat
     4441 attaacataa tctaataaga taaacataga aaaagtcaga taaaatttaa ttttatctga
     4501 ctttttcatt taaaaatgct ctcttgtgtt ggtgattcag aatagtatgg tactcgtatt
     4561 tgagactaat attggcaaac aacataaaga aaaatataaa ttttttataa taaactgtat
     4621 aaaattttaa taattgacca caataataaa tgtattcctc ataatattcc cccaatatta
     4681 tagaaataaa tttaagcgag attaaattca gttaatctcg cttaaataat gtctaaattt
     4741 attattccaa aaaatcatca aaatctaatg aacctgattc tgcaaaatca tataatgcat
     4801 tttgctcaaa gaaaaatttc tcttcaaatt taatatagtc gctataatta gattgactta
     4861 tttcattgat ataattgcaa tcattttcgt tataagtacc aataaaaaac ttatttccca
     4921 ttaagtaata tatagctttc atatcattat ttattatata tacattttct tttacatggt
     4981 cattattaaa attaaatcta gtaaagaata taccttcatt catattactt tttaacagaa
     5041 atggattttc tctataaaat actcctgcaa attcttcagg tgaatttaca ctgtaaacac
     5101 ttacaaattc acttttagaa atctcatcaa atacctcaaa atcatacata ggttctttat
     5161 ctgtcactaa tagagaatta attctaaaac cagtttcata acacttactt atgcaaaaag
     5221 caattttaca tatataatat ccatcattat cttcatataa agcttcactt atatatctgc
     5281 cttgaccttt atcacttacc acgtttttaa gtaatgttcc atttatatta gtaatgtgca
     5341 tatttgcaat ctcgtcactt ccagaaaagt actttaaact ttctctatcc catgctataa
     5401 atctcatggt catatagttt ataaattcaa cttcctcgtc aactctttta catattttat
     5461 tgtataattt ttctatagtt aaatctgttt tatagttttt tataatatca atatactcac
     5521 atttatctcc tggcaatagc ttatcataat aaagattctt attaccaaaa taagatacta
     5581 aaaataaaga ttcatccttc gttaattcaa ctctatctga accaaatcct cccatcaatc
     5641 tttcttcttc catataagct tcttcttgtg ttgggttatt taaactcaca taatccgcta
     5701 atcctaatcc ctctgcatct aataaaaaat attgacaaaa gtgattgttt tcatcatcta
     5761 tccaatttat tataagacct aaactgccca taagtctaga atttgtagcc tttgcaaaat
     5821 taaaattcat atagtcacat cctttaaact ctaaaatact tgttttttaa tatataaatt
     5881 attatacata tattttaaca acttagatac aatttataat ataatataag gaaatgatta
     5941 attgaggtgg tcttatgaaa aatatagcag ctttttttga tatagatggt actctttata
     6001 gagactctct tatggttgaa cattttaaaa aattaattaa atacgatata attgaccaaa
     6061 aaaattggta taaacatgct agagacactt ttatggattg ggataaaaga caaggtaatt
     6121 atgatgatta tctattagaa atatgtgacc tttatgttga ttcactaaaa ggattagata
     6181 aaacctgtat caactttaca agtgaccaag taattaaatt aaaagcagat agagtttata
     6241 aatacacacg ttcaaggatt gagtggcatc taaatcaagg acacatagtt atatttatat
     6301 ctggtagccc tggatttctt gttgaaaaaa tggcaaaaaa atataatgta accgattttc
     6361 taggaagtga ttatgtcttt gaaaacaata tatttacagg cacagttatt ccaatgtggg
     6421 attctataag taagaataat gctataaacg attttgttgt aaagtatgat ttagatttat
     6481 caaactcgta tgcctatgga gatactaatg gtgatataaa tatgctaaaa agagttggaa
     6541 atccaatagc aataaatcca acaaaggaac ttttatctca aatatcactt gatgaggaaa
     6601 tttctaaaaa tgttgagata atagtagaaa gaaaagatat tatttattct ctatctacag
     6661 atgtaaatat agtggatatt taatttatac tatctatttt aaaaacttat attatatcta
     6721 atatttatat ctaacatata aaagctttga ctaaaaatca aagcttttat atgttgcact
     6781 aagaatatat taaatttagc tttatgcttt aatgtatatt catatatatc atttacactt
     6841 atctaaacta caatttcatt aaaattaaat ataaaaatca actattattt tttaaatagc
     6901 atctttctta atctattatt gtaataacct ataaaagaac tgtaaacata gtcatatact
     6961 ataaaggcta tctcaaatga acctataata aatatgttta ttggaatata aactattgtt
     7021 cttaataaaa aatacaatat aagtatcatt atatttgcaa atactaattt cataaaatac
     7081 tcaatatata tagacctatc tttttctata agatatttta caataccata tattccaaag
     7141 gtaagtgaat atacaatcca ttgagactta cttgccatca ctataaatcc taaaactata
     7201 gaacctatat aaaaagcaat tccactctta aagccccatt ccattattac aattgatact
     7261 aaaagtgatg ctaaccccat aataaacaat gtatttacag gaataatgtt tgtaagagtc
     7321 aacaatatca catttaatgc taataaaact cctccataag caacttttat actcatattt
     7381 atcatcctaa caacaagtta ttaaatctcc accaaaacat tcacaacatg aatctaaaca
     7441 ccaaagttta gcacaatttt cacaacaatc gtcacctaga caacaacagc aatcatcaca
     7501 gcaacagcaa caacatccat ctaaatcatt acgtcttctt ctattataat tgtcagccct
     7561 attgctataa tcatttctat attgtgtata acttctaagt gcatcactat attcactatt
     7621 ttcaggctcc ataaattttg ctctttttaa gaagtcttca ccttcttcat aatatcctat
     7681 gttcattgca gaaaaaccag ttaaataata ccactctgca catttgtcag tcaaagtttt
     7741 taaaaaatca tatgcttttt taaattcact attttctatg aatgatcttg ctctcataaa
     7801 attttcatta ttataagtat tactgtacat tctttccacc tctactttct aatatattaa
     7861 tatatcttaa ataaacacca gaataaatta tattttctat tattccaaca tttttattta
     7921 gatttagatt atcaattccc ctagctaaaa atcctaatga tgttactatt atttttttta
     7981 ctttttcttt taactcatca tttttatcaa tatactctat aaatggatta tatcttcctt
     8041 tcttaaaatc ttcatctaaa tcttcatagg catctagtag atatatatac ttacctatat
     8101 taaaacctat cattcttaag tttctctcat tttcatcttt tttatagaca aatacttcac
     8161 ccattatatt tccaaatata ctagatactt catctatatt tatattttta tctttttcta
     8221 acttatttaa ttcatttagt tgttgtttta tatactctgc tttttcagga tacttttcat
     8281 atgctaattt taatttgttt ttatatatat aatatgccaa tttatctttt atgcgtttat
     8341 catccattaa attatcttcc aatttataat acgttaaaag aatattcata cttgctgcat
     8401 attctgtaat atcatttgta attacctttt tctttttaaa tggatttact atacacactt
     8461 cctctgtact tatagactct gggttatata ctgctgttaa taaaacaatt aaaaatgtta
     8521 tatcatagtt caaagaaaac ctagagattt ctccatgatt tctttttaag tatttacata
     8581 gtccacaata gtaagctttg taataatcat attctctaaa tgttaaatcc attttgttta
     8641 ttttaacata tccaaacata aatactcctt tttgtactat tttttattta gattatatat
     8701 agtataacac tttttatcaa gattatataa tttacagtat tctaagaaac ttaaaaaatt
     8761 ccttctaatc attgtatctt aaatttaaaa taattagaag gttgggttaa aaataatatt
     8821 aaattatata cttatattaa ctcatacaag ttagttatgc ttatatcaat ttaagtttta
     8881 aataattggt atatatttaa aactacctac agtatccttc aattcttttt ttgctttgtt
     8941 tataaccaat tcatcaagca ttacatcatt tattgaacat gatatagatt tagatgccaa
     9001 taacattgcc ttaaatccca tatcagaacc tgcactggca caagattgcc atgtatgggc
     9061 agaaatacca ataggccaaa ctgaacaaga acactgtgct gtaggaatta tgtagctaac
     9121 atctccaata tctaatgata atgatataca gttattatga gtaaatgtat catccgtaac
     9181 ttcatcatgc agattcatct tagtaatatc cttatttcca cttaatacac tagaaacact
     9241 acttctttta tctatagaca ctgtctcagc taatttatct gcaaaatagt agtcatcttc
     9301 attatagttt tgcacaccta ctaatttcat attttgtgat agaatatctg ttaatattgt
     9361 atttggaata tagtcatata ctccacttat aatgtcatat tccataatag taccagtcat
     9421 taatgctgct ccttttgcta tatcaacaat cctaccaaat acatctctta catgctccat
     9481 tttaactcct cttatgtaat accacacttc tgcaaaatca ggaactatat ttggcatttt
     9541 tccaccatta gtagttacat agtgtactct acaattatca aatatatgtt ctctcaaata
     9601 attacaacct atattcataa gttctactgc atcaagtgca cttcttccgt tataaggagc
     9661 ttgtgctgca tgggcactaa tcccattata tctaaatttc acagagtaat tagcaagtgt
     9721 tggtattctt attggagtat ttatatcaaa tggatgccaa ctaaaagcac aatcaattcc
     9781 atcaaaaaaa ccttttttta tcatcaatac ttttccagat aaatcttctt ctgctggaca
     9841 tccatagtat tttatagtcc ctttcagctt ttttagtttg attaattcct ttactgcaat
     9901 tacagctcct attgaacctg taccaagtaa gttatgtcca catccatgac ctgcaccacc
     9961 atctacaata ggttctttag tagtggatac tttttgagac aaaccatcta aagcatcata
    10021 ttctcccaaa actgctataa ctggtctacc ttctccataa ctagccacaa atgctgtatc
    10081 cataccttct aatccctcta tactaaaacc atgttttttt aaacattctt tttgtaaatt
    10141 tgaagcatag tattctttat actgaagttc tggatttttc catatcttat ctgatatatc
    10201 tcttaattct ccctgtatct catctatata ttccatcaaa aactttttat ttaaattatc
    10261 catattaatt atataaacct ccctgtttct aataacttat cgattatcag ccaaaatgac
    10321 taattttaac tgatttaaaa atattttcta ccatacaacc attttataca ataaaacctc
    10381 ttgacttgta agtttcattt tatcaagtaa gaggttttca agttttataa aatttttagc
    10441 atgttaacca atcaagcata ttaacccata atttattata gtatttccaa tcagtaaatt
    10501 cttttggacc ccagtgaggt gcacagtcag aactaaagat agcagatttt ccttttccaa
    10561 actcgccaac agctacaaat gggtctcctc caatagttgc taaaacagga caattatctc
    10621 tagctactgt tttgttatat cctaaaaact ttggccattc tgttggtata ccttttaata
    10681 ctggatgttc agagtcaata acttctggaa ttattcctgc tggtttttca actctatcat
    10741 ctttatctat cattgttatt ggtaatacat ctttgatagc agtttctcca aatctagctt
    10801 ttgcatctat tcctgtaaat gacatatatc cacctaccat gattaacgcc ccaccattat
    10861 ttacatactc tttgattagt tcaagtctat ctggattact attgcaatct ataaaagtac
    10921 tattagataa taagaacgta ttagaaccta tatcagataa tattatacag tcatactcat
    10981 ttaactcttc taatttatac ggaaaactat ctgctgctac atgtgcaggc atatgaactg
    11041 tctcatatcc tccagattct attgcttctt tcaaccactt tacagcttct tcataatgag
    11101 ttgtaacaaa cgtatcaaac ccttttatat gagttatatt ttttacccaa gattccccta
    11161 ctattaatac ttttttcatt tatatattcc tcctactttt ataagtttaa tattagactt
    11221 gcatacataa gtatactatc taaatactcc ttaatttcaa catattcatc aattgaatga
    11281 cattgttcta ttgaacctgg ccctaatata agtgttggta tttcagctaa atttttcatt
    11341 actctcgcat cattgccagc tgttgaacct tttatctcta atttttttcc tagagtttct
    11401 tctagtattt tatgtgcatt cactacaaaa tcagaatcct tgtccatctc aaaacctaac
    11461 cctgattgat atatgtttat atttggttta ttatctttaa gccataaatc tccattagcc
    11521 cttgtcatta tgacatttgt tacatcattt acaacttgtt cataactcat tatatttggc
    11581 aagaagtgta cacataaatt aaatacacac ttatctggca cagtagaacc tgctgttcca
    11641 ccatttatta cacctaaatt tattgtaggt gaaggtaata gtggatgttt atatatcata
    11701 agccagttat gttccaactc ttttatatct tgaagcaaca acatagcctt ctcaattgca
    11761 tttacaccct cccacttact accacagtga agagacacac ctttaacctc aacttcaaag
    11821 aatacaaatc ccatatgtgc aactattaag ttttgttctg atggttcaca aattatgcaa
    11881 caatctccat caataccatt cataactgca tttatggtac catttccgcc accttcttca
    11941 tctactactg acattatttt tacattacca ggtacattta gaccactatc ttttataagc
    12001 tttacagcta atattgatgc tataagtcca gatttcatat cagcagttcc aagaccataa
    12061 agttttccat tatcttctgt agcacggtat ggattatatt tccacttact tatatcaccc
    12121 ggtggcatag tgtctacatg accattaaat acaattgtct taccaggtaa atcatcacta
    12181 tacttacaaa ttaaattata tctatcttga taattgtgac ctagatttcc ttctttatat
    12241 atattttttg cttttttaat taattcttcg ctcatttctt gtctatccac actaaaacct
    12301 atgtaatttg caagtttttc aatatattcc tgtccttctt tttcaaatcc acctagtatt
    12361 ccatgaccaa catcttcagt ctttatactt acaagttctt ttaaataatc tatgtattct
    12421 tgttttttac tatttaatgt atctaagaga atttttttca tatttcctcc taagatttat
    12481 ttttacattt aaaatttcaa gtctaatctc atctatttaa gccatagtaa actttttcac
    12541 ttagcatatt ctcattcaat gtataaagat gtatgcaaat taaagtataa aaatatatac
    12601 aaaaacttaa taatttttat agtttataag ctaaaattta attactattt agatttagct
    12661 gattttttta attttatatt atttatccat atagcaaata acattaccaa accttttata
    12721 atcatttgat tatatgaaga tacacttaat aagtttaatc catttcctaa tattcctatt
    12781 attccgacac ctatacatgt acctactata gaaccagaac cacccatcat attagtacca
    12841 ccaagcacaa cggctgctat agcatccaat tctgctccat ctccaacagt tggtgaacct
    12901 gatgccaaac gtgatgatag tattatccca gctaatgctg ttagtagtcc agctagtgcg
    12961 tatactttta ctataacttt atttactttt actccacata atctagcggc ctcttcattt
    13021 ccacctactg catgtacatg acgtccaaaa ttagttcttt ttaatagaaa atgtcctgct
    13081 attacaacta taatcataat tataactggt attggtattg gtccaatata tcctctaccg
    13141 agcatcttaa atgcatctgt atttactgat ataggtcttc ctcctgtaaa tacaaagctt
    13201 gccccactta ttatcgttaa catggctaaa gttacaatga atggaggtag tttttgtgtt
    13261 gaaataattg caccttttat gactcctaat attgtcccta ttgctaatgc aagtatcata
    13321 cctacccata taggtgcatt ataattcact actgttgtag cccaaaatgt acctactaaa
    13381 gcaattactg ctcctacact taaatctatt cctcctgtaa ttattacaaa tgtcatacct
    13441 actgcaagta ttgcatttat tgatatttgt cttccaacat taattaaatt atctaaagtt
    13501 aaaaagttag atgaagctac actcattatt ataaaaatta ttacaagtcc tacagctgca
    13561 ctcccttggt ctctaaatac acttgttaat tttgtcaata atggagaaga tttttcttct
    13621 ttatgcttag ttattgaact cattaatttt acctccgaaa gccaatttta atacattatt
    13681 ttcaaccatt tcttcacctt ctaaaaatcc acttatatga ccttctctca taaccattat
    13741 tctgtcactc attgatatta attctggtat ttcagaagat atcattatta tacttacacc
    13801 actctccact aaatctccca ttagattgta tatctctgcc ttagcattta catctatacc
    13861 tctggttggt tcatccataa tcaatatatc tggttgtaat aaaagccatc ttgctattat
    13921 agctttttgt tggtttccac cacttaaact ttgtataact tgattcatat gtggtgtttt
    13981 tatctttaaa gcttctatgt atttttctaa aatctctttt tccttttttc tatctataat
    14041 tgatttaaac ttagatatct tttttaaccc agccatagtt gtattttctt tgacactcat
    14101 ttgtaaaaac aatccagtac cttttctatc ttctgttatt aatcctagtc cattttctat
    14161 tgcatctgat ggagttttaa actttacttc ttcattgttt aaatatattt ttccagaatc
    14221 atatgttcta agtccaaata tagcttctac tatttcactt ctcttacttc caactagtcc
    14281 acctattccc aatatttcac cttttcttat attaaagcta atatcattaa aaacattgtt
    14341 cttagttaga ttttccactc gcataatttc ttcttttatt tctacttttc tttttgggaa
    14401 aaattggtct agctttctat ctatcattaa attaactaat tcttcctcag acgatacatc
    14461 tttggtattt aaagtcgaaa tcgttttccc atctcgcatt atagttattc tgtcacaaat
    14521 ctcaaagagt tcttccatcc tatgggatat atatattata ctaactccac tctcttttaa
    14581 atcatttata agtctaaaaa gtatttcagt ctctttttta cttattgaag aagtaggttc
    14641 atccattatt aatactttag tttctgatga taaaactctt gctatttcta ccatttgttt
    14701 ttgagctata ctaagctctt tgactaaact atttggattt atatctattc ccatattatc
    14761 gaaaatcttt ttagcctctt cattcatttt tttgtcattg gttaagactt tgccatttat
    14821 ttcttttccc ataaaaatat tttgtgctac tgttaattcg tcaaaaactg ttagttcttg
    14881 atatatggca tttataccta tctttttaga gatatctggg gatatattct ctatttcttt
    14941 gccttcaaaa ataaattttc cagaagtttt cgaatgtgca ccagttaaaa tctttatcat
    15001 tgttgattta ccagcaccat tttcacccag tagtgcatga acttcgccat gaaataactc
    15061 aaaattaaca ttatctaatg ctctgacacc aggaaattct ttagcaatat tacttaactt
    15121 cagaattata ttactcataa tgacactcct ttttaaatag gatttctttc aaataatctc
    15181 attttatttt actaattctt aaagtctttt atattttcct tatcataaag taaagctggt
    15241 actggtatat tcttttctac cttttcccct tttatagctt ttactactgc ttctacagct
    15301 aaacgtccca tctctttagg ttgttgttga atcatagcaa gaatagttcc atctgttatt
    15361 ccacttgatg cttcttcagt taagtcaaat cctacaatct taacatcctt attttttgca
    15421 gattcaagtg cagctttagc tcctagtaca ctattttcag ctgttgcata tattatatct
    15481 acatctggat tagattgaat taagttttct actgtattaa gagattcttc tctgtcatat
    15541 cctggttgtg aatttaatat cttaacattt gctgaacctt ttaatgcatc tttaaatcca
    15601 tctattctct gcatttgtat ttgagatttt aaatctgtca ctattgctat ttctgacttt
    15661 ccacctaact tttcatctat gtatttttta gtgtattccc ctaatttttt accagcatca
    15721 taactaactg ttcctacata acttgtaata ttttcagtat ttaatacagc atctactgta
    15781 actactggca ttttttcaaa tgctccttct actgctggta ctatagccga attatcagtt
    15841 ccaagtacta tcattccatc tacattttgt tgtataaaat cttctattgc tgatgtttgc
    15901 ttagctacat cttggtctgg gtcatttact ataactttca cacctaattc tttagctttt
    15961 tcttttattc cctcatcaat atctataaaa aattgatgtt gaagagttat tgtagatact
    16021 ccaatagtta aatctttctt gtctgaacca cctttattat ctgatttact tggtccttcc
    16081 attgaacacc ctgttaacat tcctattgct agtattccag caattcctaa agataaaatt
    16141 tttctcactc tcataattaa cctccatttt taatagtttt aaatttgaac aaattagatt
    16201 tttatttatt atttaaatat ttttcaacat cttctttata aggcatacca gattgtgcac
    16261 ctagtttagt tacagacaga cctgatacta ctactgcata ttctactgct gtatataaat
    16321 ttttcccttc tgttatagcc actgccaatg ctccattaaa tgtatctcct gctcctgtgg
    16381 tatcttgaac atcaacactt atacaagata catgcttgat ttcctcacca tcataaaatc
    16441 tagctccatt ttttccttct gttattacaa gtttatttgg atattttttt aatacttctt
    16501 ctatcccttc atttgtgtca aaaactatct tatattcatg ttcatttggt gttaagtaag
    16561 ttactttttc tattatatct tcatttagtt ttactgctgg tgctggattt aataaaaccc
    16621 taatcctatt ttcaaaacag aaattcacta cataatttat tgtttttaat ggaatctcta
    16681 attgtagcaa tactatatct gcattcttta tctcttcttc attcttttta attaattcta
    16741 tatctactag ctcattagtc cctggtacaa caacaatact attatcattt tcacataatg
    16801 ttataacagc aacaccagaa cttttgtgat tatttctgga tactaagcta taatcaactt
    16861 tattcgttct aaaatttctt attaactcct ctcctaagcc atcttcccct atgcatgata
    16921 tcatttttac atttgctcct agtttagaag ctgagatagc ttgattagcc ccttttcctc
    16981 caggtgatgt aaaaaaacta ttacctaata ctgtttctcc tttttctggt cttttatcta
    17041 cagaacatac catgtccatg tttacacttc ctatgacaac tatgtttccc attacattac
    17101 tcctttacat atctatttat gtctctcgtg ctgttattca ctttcttttg ttgaatctct
    17161 tatgaccaat tctggtttga aaatcaattc atcaaacgat tcttttttat ctcctcttaa
    17221 acaatttatc aatatttcac tggctttata tcctatatca taagacggct gttttactgt
    17281 ggtaagagaa gggcttatta gacttgatgt ttgaatatca tcaaatccca cgacacttat
    17341 atcatttggt atacttaatc ctctttcttt aagacctctt atgacaccta ttgcaatcaa
    17401 atcatttgca caaaatattg catcaaaatc tatcttttct aaatttttta taaattcttg
    17461 tccccattca atcttatatt ttccttcaaa tacataagca ggattgtagc ttaaattgca
    17521 ctcgattaga gcctttttat atccttctaa tctatctact gctatttcat tttgaagtgg
    17581 accacttagg tatattattt tcttatttcc aatatctaat aaatgttttg tagctatata
    17641 ggccccttca aagttattta ttttaacaat tccttttaag gtttctttgt tcatacttat
    17701 cttcttatca atcaagacag ttggtatttt tatatcttta taatttgctt ctttccagtt
    17761 tgtatttgaa gatgctgcaa atataattcc atctatcatc ctctctgata gtgttcttat
    17821 tccttcatat tcttttttag catcttcatc tgtattacat aaaattatat tgtagccttc
    17881 ttcattagct ttgtcttcta cacctcttga tacatctgta aagaatggat ttcgtatatc
    17941 tggtataaga agccctatag tattagtttt tttagttact aaactttgtg ctaatttatt
    18001 tggtacataa tttttttcct tcattatttt caaaattttt tctcttgttg cctgacttat
    18061 attttcatct ttattattaa tgacttttga cacagttgtt ttagaaacac cagccaattc
    18121 gccaatttct tttatagtta tactcaagaa gttcaccccc aaaatttagg aaaccgtttt
    18181 acttaaccgg ttacctagat actatcacat cttttttcca tttgcaacca ttttatataa
    18241 attttgatat ttttcccttt tttcaaaata ttgcaactta taattcaatt ttaatattta
    18301 aattaatctg aaaaaaaaat taatatactt ttataattat ctaaaagtat attaattaat
    18361 ttaagcagta tttattaaag ttcaaatcct tgctctataa tcattttttt atcattttca
    18421 atcccacttc caacagttgt caaataatca ccaacaattg cagaatttac tcccccatta
    18481 aatccaactt tgtcatatcc ctttaatgat agccttccac cagcatacct tatgtacaca
    18541 ttaggaatta taaacctaaa tactgctgtt gtttttagta tttccattgg ttctaataca
    18601 tcataatttt ccattggtgt attcttaata ggatttaaaa tgtttattgg aaatgatttt
    18661 acatttaatt ctcgtatttc aaaagccatt tttagtctct gttcttcact ctcattcatt
    18721 ccaattatac cgcctacaca tacatctagc ccagcttttt tagcatcttt tatagttttt
    18781 atcctatctt tatatgtgtg tgttgtacat atattatgaa aattatctct acacgtctca
    18841 acattgtggt gatatgtagt cacacctgct gacttcaaca ttttagcttg ctcataatct
    18901 ataataccta aagatgcaca taattttaaa ttagtatttt ccctcaatcc ttcatagata
    18961 tttaaaatat tattaaactc ctttcctgac atacctttac cacttgtaac caatgagaat
    19021 ctatgcactc ctttactctc catctcttta gctctattta agatttcatt gtaatctaat
    19081 aatgaatatt cttctacacc agttttataa taagaagact gagcacaaaa tttacagtct
    19141 tcactacatt tgccagattt agcattcatt atcgtacata aatctgcttt tcttcccata
    19201 aaatattctc ttatttcatt tgcagatttt aatagaatat caaaatcgtt tttattattt
    19261 gtatcaaggc ttattaaatt aagagcttct tcataactta tttctttttc tctcaatact
    19321 ttatttttta attttattat gtatttttcc atatatcctc ctatttgtca acttaatatt
    19381 tttatgtagt ttacttatta tatatagtat tgcatatata acatttattg gtcaacttta
    19441 ttttagacac aagtttacta tacacaaaaa aatactgctt caaaacaata ttagtatata
    19501 gattatacat attattatta aagcagtatt ttttattaga atataattct tactggttta
    19561 taaaactatc cttgtcttaa ctccatccaa gctttccatt gtaataactt ctaagccttc
    19621 atattctcta aatagccatt ttaaatcctt catggcttga ttaacctgac tttttgttag
    19681 attatttata aaatattctt ttctagtttc ctcatactct ttgctagtca aaaagtttct
    19741 tagaaaatta aacgcaaata cacttggaat attgtttaca ttagccttct tgctgttttc
    19801 cttctcatag taaattttca taaatgcata ctcctttgat acttttagat ttctcttaca
    19861 ataattatat gcatttaagt gctactattc ttataatttt attaattcat attctttact
    19921 tcccataccc atttcacatg catagtctat tattctagtt ccatttacat gaggatgttc
    19981 atgtttgaaa acatcatgac ccatttcctt acaaatcaaa tcatagcatg cttgttcaat
    20041 agctacaggg tctgttgatg ctaatattcc tatatcatgg gcaattggtc tacctgacca
    20101 aggtacacaa tcacaaagtg gagttacatt cataacaaaa gttatatacc ctaccttgtc
    20161 ttttttattt gatactgctc cataagcgta ttctgccatc ttttcgacaa acacatctga
    20221 gtcactttcc cattgtatag ttactgctct agttggacag acagttggac attctccaca
    20281 tccataacat acatcagaat ctataactgc ttttttatcc actatactta tagcttttgt
    20341 aggacaagaa ttaacacact ttccacaacc tacacacttc ttttccttta ctactggagt
    20401 aacatcagaa tgttgcattt gttttcctgc tgcacttgca catcccatag ctaagttttt
    20461 taatgctcct ccaaaacctg ctgcttcatg acctttaaaa tgactcatta ctatcattgc
    20521 agaagaatta taaatttctc cacctatttt tacactttca aaatgttttt tgtctatttt
    20581 tacattctca taatttctac tatataatcc atctgcaatt attactggtg catttacaac
    20641 tgcataagca aatccatttt ctatagcagt tgtaagatgg tctacagaat ttgttctact
    20701 ccctgtatac agtgtatttg tatctgttaa aaatggcttt ccttcgcagt ctttaacctt
    20761 atcaaccact tgtctaactg ccacaggact catatatgta gtatttccct tttctccaaa
    20821 gtgaagttta attgcgactt ggtcattttt ttctattaaa tctttgaatc ctgcttcatc
    20881 aaataatctt cttacattgt ttggtacgtt tttattttgc gaatttgagt ataaatcaca
    20941 aaaatatact tttgactttt ccatatctac acccctttaa aatatttact tatactaatt
    21001 ttatcacttt taaagtaatt tttagtatat tttaacataa ttaaatattc ttatatttat
    21061 ttgatacaaa taatattatt tgtattattt tctacactta attctatata ttaaactaca
    21121 cctaaaaatc aaaaagactt gaaacttaag tccaagtctt tttgattttt aggtgtagtt
    21181 tttaacaaca taagattact ttaccttgtg ataaaggtat tattaaactg gttttttatt
    21241 aaataaaata acagctgcta gtaaacatac tatagaaata agaactgtta taataattgg
    21301 aataataaat tctggcattg ccactttccc tgaaatcaag tctatatttt ttgacatcaa
    21361 gaaattagga gtataaggtg caatctgttt aggaatagaa attaaactca ataagagagt
    21421 tgctaccaat acaagtaaaa cactggcaaa tgctggtcta aatagtacac acccaagaat
    21481 taatacacta atatacataa ttcctataag ccataaatta aatgctgaaa atataatatg
    21541 attaagattt gcagttggcc aaaaatacat tgtatagcca taagcaaata aaaaactcaa
    21601 ccaaaaacca attgtcataa taatcaccgt aacagagaat ttagacaata ctatagatga
    21661 acgtgataaa cctttagtca gcataataac aagtgtccct ttagaatatt catttgataa
    21721 aatattacaa aaaataatta gagttaaact cataccaaga ccagagatat tgctaaaaaa
    21781 ttgtgtccaa gaatctaaag cagttggtgc agcagtaact tttaaagtcg gtgcaaaatg
    21841 agcaatcaag tctggcataa attttgcaga aataggacta cttataccaa atattagaaa
    21901 taaaataatt aaactaaata atttataatt tcttaaattc tctttaaatt ctttttttgt
    21961 aaatgctatg tattctctca ctttactacc tccaaaaata aattttcaag tgataattct
    22021 attagttcca tctttactgg acaaagattc atttctgcga gcgtagatat aactgttttt
    22081 tgtattgctt ttatatcttt accatgcaaa acaatttcca tattagtttc ttctgaatct
    22141 tttagtaagg atttaatacc atcactagac ttaaactttt gaatttcatc attacttgca
    22201 aactccagta aaagtctatc ctttccgtgc atacttttta tttctgaaag tgtaccacta
    22261 agggcaatgc ttcctttgtt taatatagca acatggtcac agattctttc aacatctgat
    22321 agtatatgtg ttgaaaatat tacagttgtt gagtctttga ttttaagcat aatatctaat
    22381 atttcttttc gaccaacagg gtcaagtgca cttgttggtt catcacaaat aagtaatttg
    22441 gggcgtgaaa gcaaagcttg agctatacca agtcgttgct tcattccacg agaaaaccca
    22501 cctattcgtc tcttttcatt tctaagaccc acaagtgata acaactcttc actccttatc
    22561 tgtatttctt tttttgaaag tcccgtaatt tcaccacaaa gagaaagata ttcaagtggt
    22621 ctcatataat tgtaaaactc tggaacatca ggcagataac ctacatgacg attagaactt
    22681 gttttcccat aggtcacttt ctcaccacaa acatctatag ttccactatc tggtttaagt
    22741 aacccaagta ccattttcat tgttgtagtc ttacctgcac cattcttacc tacaaaccca
    22801 aagactgacc cttctggtac agtaaaattt aaattatcta ttatctttcg attaccaaaa
    22861 ccttttgata atccatttat tgagacaata ttcatcattc ttcacctctt cctattgtaa
    22921 aatacaaaat tggtcctatt aaactaataa acagtactac aataatccat attgccttat
    22981 ttccaatttt ataatttgga tgtctcaaca catgaacaag tgcagtaatt ataagtatta
    23041 aatctaaaat aataacagga accaaaagtg gtaagtattc catcaaattt tccataatct
    23101 tattcctcca ataaagataa tttttctata agaaatttgt attctttgtt ttcacttaat
    23161 acagaaaaca ttggattatt tttaatagca gcaacaatac ctgcttttac gattgtatca
    23221 tcacgtgtaa tacctgttcc aatttcagaa atccatctgt caagtttatt aaaaaagtca
    23281 tccccatgta ttataattgg gtatatatca cgtgttgcta aatctacata ttttcgtaat
    23341 gatttaatag ctccttcatt atcaccatgt attaaattaa tttgtgcctg tgttaaatat
    23401 gaagaaagca taatagcagg agataatgta tcaagctcaa atatatctga aattgctaga
    23461 atacgtcttt ctatttcctt catttgtaat ttatctgcat tttgcaaaat acccactaga
    23521 ttttggacta atgatataag gttctgatat gcaccaagtt gaaatgtttc tcttgcttca
    23581 tccatttgac cattcatcat ttgcccttgt gcaagaagta tagattcatt tatcatggga
    23641 aaattgctgt tttgcaataa atcaatcact tgtatgggtt gatttaacaa tatataacaa
    23701 gttgcctcca tactttttgc ttgacggcaa atatctatgt cattacaagt ttcctgtata
    23761 cgaataaata tttctaatgc ttcactaatt aatagttcac gctttttttc atctacaata
    23821 tcatagtgat taactattaa aagacccatc cgaaaaatca aaggaaaaca tgaataatat
    23881 ttttttatga tttctcttat ttcatccatg acttcatcaa atggtttaac cgtaaagtct
    23941 ttgcacagtt tattatacag tttattaata tcttccttca tcatttgagg ttcataacat
    24001 attaactcat caacggtaat gttgaaatat gtcgcgagtt gcggtaaaaa ggttatgtca
    24061 ggatagcttt gtccagtttc ccatttagat acagaagctt tagaaacgcc tatataattt
    24121 gcaagttcat cttgtgtaat tcctttttct ttacgcttta aaatcagcgt ttttgcgatg
    24181 tttaattcat tcatgtcaat ccctccttat gtataattat atccttaaac atgtaaagct
    24241 aacaagcgat ttgaagttga cttttgacga aaaaatcaac cacagagaaa caaataaaca
    24301 agtatataaa atatatattt aaatttatct ttaatctcct ttgtaaaata tgtaaacttt
    24361 gtaaataaaa tccatattat ataaaactaa tagttaatct atctaaatac agtttttatt
    24421 tatcacgttt ttaaatattt tgtgtaaaac ctcttttaat ataaacacct tattttcatt
    24481 tctcatttca tattcttttt aaaatactga atataactct tcgattttcc atttaatcct
    24541 tttataaagt tattattcgt atttattcct ctaattataa caggcaacta tcatgtattg
    24601 gtatttgtct attatataaa aatctataaa agaaaagcag tcaatcattt agattaactg
    24661 cttgtacata tatcttatta aactgtataa actataattt ttattattct ccattttcat
    24721 cccaagtcat aaatccccat ccactatctg gataaatatc gatagacttt tctctaatca
    24781 tataaccaac tttataacac tcttttgtta ttttattaga tagtatacat ccttctccat
    24841 ttatctaatt ccatttaatt aacttttctt ttcaacctta acttttataa aatcatcttt
    24901 tttaataatt tcatacttat tttctcttca acatcatcaa atgagggtgt ctcaaaatta
    24961 actttttact tcataaggca ctcttttttg tatgaagtaa aatatatttt catcatttca
    25021 acaataagta agaggcttat ctctattttg agacagcccc atttgatggt gttgaacatt
    25081 taaataaggt tgaactcgtt ttatctctct ttttaagata tctgtaaatt tgcacataaa
    25141 gtctcttctt cttttacaat tctaatagtt ttattaaagc caaagtatat atctccatat
    25201 tctttcgtat atgatttatt ggtactcttt catctactcc atgaccacca gttttatacg
    25261 ctaagaactt aggtccaaac gccaccatat ctttaaacca acttgcatat gtgccaccac
    25321 ccatagcaat tgtatatcca ttttcatctg ttacttcttc ataggcttct aataattttt
    25381 taagtactaa attactctca tccacataat gtaagtcttt atgaacaata acctctattt
    25441 tccaaccatt acaaaaaacc tttttgaatc tatctattat attgtttaaa actatatttt
    25501 taccatacct tatatctaac tcacatacga ttttttcctc atttactttt aatattccta
    25561 aatttaaggt agtgtttttg aatacctcat catattgatt aatccccaat gcatctccat
    25621 aataatcact acaaaggtat tttgaaacta attctatatc ttctctatgc tgaaaatcca
    25681 tattatcctc aactatcttt attaccattt tagatatagc attttctccc ttttcaggat
    25741 tacttgcatg tacagctact cctttagtag ttaactctga aataattcca ttaataattt
    25801 ttgcagtaca atgagatgga acaacatttt ctttagttcc tccacttact attatgtaag
    25861 gtttactttt gtcaatttta atttcttttt ctatccttat atgaaccctt cctttttctc
    25921 cattaaccac tggatacata gcatctggag taaatcccat acttggaatt tcttcacctt
    25981 tatctaagta atatttgata catttcatac ctgtctcctc atttgtacca aaaattaatc
    26041 ttattctttt attaattgta ggatacatat cctctatgtt tttcaataca tgtataagca
    26101 tagatacagc acccttatta tctatgacac ctcttccata aataatattg tcaattactg
    26161 ctcccgaaaa tgggtctaca ctccacccct ctcctggtgg aactatatct aaatgcattg
    26221 gaatccctat taattcctct ccctctccaa tttctgcata tccaatataa ttatcaatat
    26281 ttttaacact aaaacctaaa ctagcacagt aatctaaagt tttatctaaa gattttttag
    26341 gtcctatacc aaaaggacat tgtgaaatag gttcaccttg ttgtgaatca atttttatca
    26401 attcttttag aaattctatg gttctattta aactattatc tacacttctg cttatatcca
    26461 taaatcaacc tactttctat ctttttaaag cctgtcctta aagaacaggc ttttagcatt
    26521 aaaaattatt tttttgtaaa tactaattca caaacttcat catcatgagc acatttttga
    26581 gcaaaatcta atttaacacc ttgtgcacag tcaactcttc cataatcccc ataacaagct
    26641 aatctacaaa tttcttttct ttcttcttta gttaatccat attctttcca agcagcatct
    26701 aatggacagt gatggaattt taaaacgcca ttctcatctg tattttctac taattccatt
    26761 tcaaacactt tttgtccttt actgttatat aacatttctg ccatttttcc tggagtatct
    26821 gctacgttat atttttgacc tctcatttta ccaaatccat atattgcttc tttacatata
    26881 tcatctggat tagctcctaa tttttcagct tctttcataa gaagatagaa ccatactgct
    26941 ctttctcctg ttgctcctct aattccttct aaatattcct tttctgtata cattttttct
    27001 gacatttctt tctcctccta aaattaaatt ctatatttta atcactgtga attagaatat
    27061 tccaatgaat gaccctacca atgctaatac aaccaaaatt actaataact tagttattgt
    27121 ccacttcttg tatttcattc cccaatatac gaagaataca acaccaagtg gtaataaacc
    27181 tggagcaatc atatttaatg tatcttgaac aacaattgct ttaccaccaa tatcaaatga
    27241 tagcggagtt gaaagcttaa ctgtagttgc agataatgct cccatcataa acattcctag
    27301 aattgaagca ccatcaatta tcttattgac aataccacct gatagaattt tcttaattga
    27361 atctctacct aaagaatatc ctaaatgaaa taagtaataa ccttcgcaaa atagtaatat
    27421 actaaacatt attgggaaaa atgcacctat aacacttcct tctgctccaa atgaagcacc
    27481 taaagctaag aatattgttt gaaatgtacc aaaatctaaa gtatctccaa ttccagccaa
    27541 aggtcccata agaccattct taacacctga aataacttct cctgggattt ttccttcagt
    27601 agctttctct tcttccatag ctaaaactgt accgtgaatt aatcctcccc atatagcttg
    27661 agtattaaaa aattgtaagt gtctctttaa cgcatctact aaatcttctt ttctcttata
    27721 taacttttct aaagcaggag tgaaacttgc acaaactgct aatgcttgca ttctttcaaa
    27781 agagttggat aattctgctg tccaccacca tcttagatat gtttttgtta catctttctt
    27841 tgtaagtact cttgtcttat taacttcagt tggaccttgt ccatttttaa cttctttatt
    27901 ttttatttct tgcattatac ttcacctacc cttttgtctt ctttcataaa ggtaattaaa
    27961 agagctatac aaacaccaaa tattgcagct gcagtaattg atatttcaaa atattgaacc
    28021 ataaaaaatc ctataataaa cattggtaag aatttatttt tacctatagt aaatattgtt
    28081 gttgcaaaac ctaatgcagg taatataccg cccattactg ttaatccatt cattaaccat
    28141 tgtggtataa catctaataa tgattgaact aaatctgctc caaaaaagtt acatacaaaa
    28201 actattggaa accttaaaac aaacccaaat attaaagaat aaagtgttgc acatctccag
    28261 atgccttttg tattaccttc ttcagcatac ttatctgcct tatgaaccaa tacagcatta
    28321 ccagttcttc ttaagttatt aactaatact cctataacac ctaaaggtac cgcaatagat
    28381 actgcaactt ctgcattaac accagtttga agtgctactg gtattgcaat aagagcagct
    28441 agacatttat ctgatggaat atttccccct gctgcgacca ttcctagata aaccatttct
    28501 atacttgcac caacaatcat accagttgtt acatcacccc aaagtaatcc aaggctaaaa
    28561 cctataaata aaggagactt tagtgttgag aaaagggtat accctgcaag acctgctgca
    28621 aaccaatacc ataatcctgt tacaattgcc aacatcaaaa cagattccat ttataattcc
    28681 tcctaattta tattttttat ttaaatttct ttaacgcatt ttcatatgtc atagatgatt
    28741 cctctggtat aacttgtagg actatttcaa ccccattttc attaagttca ttaagttgtt
    28801 ctacatcctc atttcctaat gaaacagctg taaacaccat tttctttcct gcttcatttg
    28861 gaatacctcc taattgaatc ttttcatatt taagtccagc tttataagcc ttatttgcca
    28921 tatcaacatt tttaaataga ataaatatat ttttatctcc taaagtgttg ttttggaatg
    28981 cttgtcccgc atcttctgct gttaaaataa ctacttctac tcctgatggt gctgccattt
    29041 tatatatgtc agccataaat tcatcttgac ctaagaagtc atcaactatt attatttttt
    29101 gagcttgagc gatttttacc cattttgtta taacttgccc atgaattaat ctgtggtcta
    29161 ctcttactaa tgttattttt gccattatat tcacctcata atttatattt ttttaaattc
    29221 tttaattaaa tccacacaaa ggttattatt ttttttgacg attctttctg gtaatgtatg
    29281 taatttatct tctttgctta attcttcatc tacgattaaa attgtctgta agctaagtcc
    29341 atttattgca tctattcctc ttcttatagc aacagctatt gcaacacttg atgtagatcc
    29401 tccatataaa tctgatatta taattttcac atcttcttta tctactactt cttcaacctc
    29461 tttcataaat acagaaacat ctttttcaga acttagtgat aaaacttcat attgatttga
    29521 caatccaaag tttttatttg tttcttctac taagactttt ccccaagttc catggcacag
    29581 tatgattacc ttatatttat tcattgctaa ataaactctc ctttatagcg attaatattt
    29641 tggtttaatt ctacttcttc ttcatcaggt accatttgaa ttgatacatt aatattcttt
    29701 ttaataaatt catctattaa aagaaactct tttttattaa tgtatacatt ttgagatatt
    29761 ttcttttttc ctattcctgt aggtattctt ccaacaataa gttttttgat attaatccct
    29821 tcttctatca ttctatttgc agtatctaaa tttttaatta atacaaaaac gttatcatca
    29881 gcttgattat tataaaaata tctccttgat tcacttacag ttaatattga taaatcaata
    29941 ttctttggtg ctgccatttt tatcaaacct ttagcaataa catcattggt tgtttcatca
    30001 tctactacta ctatcttttt tacacctaga tgcttaaccc atttatatat aacttgacca
    30061 tgtattaatc tatcatcaca tctgcaatat tttactaaag acatttttaa tcaaactcct
    30121 ttttctatta cattgagcta agaactttat taatattgtt tataccagtt tgtcctgctt
    30181 caatacatgc ttctgctaac tcttctaaac tttttccctc tctattaatt aaagcttcaa
    30241 gtagcattgg aagatttact ccagtaagtg catgaaactt gccacctttt ttaaggttta
    30301 aacttgcaac attacttggc gaacctccta ataggtcagc taatactaaa actccgtcac
    30361 cttcatctaa ttcatttaac ttttctacaa aatttttatt taagtcttca atattatcat
    30421 tcctatttag tttaattgct gctgttcttt ctgctggtcc tgcaataagt tcacagcttt
    30481 ttataatttc ttcactaaaa ctcccatgag tcataactat tattcctatc accaatttca
    30541 cctcactttt cttgtcactt tttatataag caattgccgt gccaacttat gtgtatcaat
    30601 aacaaatggc tattttacag gatttaatca aaacttttta ctcaattttt tttaaaaact
    30661 tattatttcg atatgtatca aaagattttt tcaacctgta tcaaatctgt atcaaaaaaa
    30721 gtaaaaaaaa gatagccttt tggctatttt taaattttgt atcaactttt gaatttatct
    30781 gtatcatatt gtgattcaaa tatatctaat atatttgcat attccatatc tgaaataatt
    30841 atatcgaatg tctcttctac tagcttaaaa ctttctttta taactaaata taattcttta
    30901 tttctactaa ttctttcttc atatgattta taaactaaag actcttttat tattactctt
    30961 tcaatcatac atgaacaatg aaatataaac tttactaaaa gttcatcttg aattactaaa
    31021 gaatagtgtt cactaattct attaattact tcttttaata taaaataagc cttttcccca
    31081 tttaaaaaag tcaaagtttt actaatatta tcaattagta atctatcaaa atatctacca
    31141 ccattatctt tttcagcata tgtaacttcc atatctccat aatgtttctt tatagattct
    31201 ataatattaa aatataattt ctctaatgtc atttctggag ttagagcctt tctagttgct
    31261 tctattacaa taggtgtact taccatatct attgctttta cttcttttcc tgtagcatca
    31321 cgaataactt tagcaaatgt taaaatagac cccatatcag ttaaaattaa aattcccttt
    31381 cctttatcaa ttttattggc tatttctaca gcttgtacta aaatatcctg aactctatct
    31441 tctaatccca tattaattgc cactgcatgg ttacaatcca gtaaagtatt ggctacattc
    31501 accatgctag atgctgtaga ttcaccatgc attatgacta atattgctat actctcctca
    31561 tttaatccca catacgataa ataaagaaat gttgctaaaa aaattgcttc atcttctgga
    31621 aactctactc catacattaa tgacatgtta gataatattt cttttgctat gttatattca
    31681 tatgctcttt ccttcataat ttcatcttta ttaggatgct ttactatatt tccaatcttt
    31741 attctttcaa ttaaattctg aatatgtaaa gctatagctt ttattaattt atctaatcct
    31801 tctcttttgc tccatttatc atgcttttgt aaaatatatt ttacagtagt tgcaattgaa
    31861 tcattaacaa tgttgttata agctgtttga ttactactat tatatataaa agtattcatt
    31921 aaattatagg aatacttctg tatgtctcca tctataacac ttctaatttg tgactcttta
    31981 ataccttctt gttgaagttt attccaagtt tcttttatca ttccataaaa atctaaatca
    32041 tattttttat tttctattaa taaattttca ctttcaaaat ccttttcttc tggtaaaaaa
    32101 ataatatcat cttttccaat aaaactgaaa ttttgtacaa actcctcttt tctattattt
    32161 ccatataatg attctctaat attatttggt agaagagaga gccttacata tacacattcc
    32221 cttttatagg tcatataatc taaaaaagct cttgcacaaa ttaattgtat atcgcttttt
    32281 aattggccta tgtttccctt acacttatat aaaataaatg ccttaataac ttcttttgaa
    32341 acctttaact taacaccaat cctaacactc tcttctttaa aaaaataaca tataagcttc
    32401 attctctctt gaaagcctct ttcctctaaa gacggaatct ttatagttac aggtattctt
    32461 cttaaaaacg tttccaacat tgctgtttct gggtcttctg tagtggctgc aattatcata
    32521 actctacact cgtgtacctt attagcctct cctaaccttc tatatatccc tttatccata
    32581 agcagaaata gcatttcttg tccctctgca gataatctat gaacttcatc taaaaacaat
    32641 actccattat tagcttcatg tacaatacca tacttatctt tatcagctcc agtaaatgct
    32701 ccttttacat atccaaaaag ctgcgctaat aataattgct gattatcaga ataatcagca
    32761 cagttaaata tgataaatgg tgcattatca ctatcttctt ttatagttaa actatactta
    32821 tacatatatt ctgcaaatat agatttacca actcctgtag gtcctattat taatgtactc
    32881 aaccccttag gtggatatat tactgctgct ttagcctgtt ctatttggga ccttaaactt
    32941 ccatcacatc ctactaaatt aaaaatattg ttctcttttt ttatactctc taaaatatac
    33001 tcatctttga tagaatcatt ttcactatat tcgtttatag agttaatatt atcaaatatc
    33061 cctttattta gtttctttcc taaaatactc tcaatacttt ctttatataa gtactgaaca
    33121 ggcttaccct ttatctttat aataatactt tcttttatta attgatttaa ttccttacta
    33181 gcattacttc tatttatacc tacttcagta gctatatatt ctgcatcaaa tccaaatcta
    33241 atgccattat taaagttcaa gtgttcttct gttaaccttt tcaagcaaat taaaactttt
    33301 tctttccttg atagcttctt ttgtgtcata gttaacctcc cctataagca gtaatactgt
    33361 taaacattta ttattaagct taattctaca atattttaat atttttgtct aattatgtaa
    33421 ctattgattt tctttcctat tcgttttata atataaatta cgttaccctc atcttttata
    33481 gaggaggaat ttatatgaat tttttatcag atttacacac ccactctata gtcagtggtc
    33541 atggctatag cactttacta gaaaatatta attattgtaa agaaaatgga attaagatac
    33601 taggtacatc tgaacatggt cctaagatgc ctggttctcc ccacagatgg tactttcaca
    33661 acattcaaaa tatcccaaga atcataaata acattataat tttaagaggc tgtgaagcaa
    33721 atatactaga tattaaagga aacatagacc tagagccttt tgtaattcct cgcttagatt
    33781 atcttatttt gtcatttcac gaagctgtgt tttctccaaa tacattagag aataatacta
    33841 aagctttaat aaatgcaata aataagcatg ataatattga aatattagga caccttggaa
    33901 acccaaacta cccaatcgac tatgaattaa tcattaaatt agctatagaa aagaatatct
    33961 taatagaaat aaataattgt tctattaaag gcgtgtcaag aaatggtagc tcggataact
    34021 gtaagtatat tgctacactt tgcaaaaaat atggtgctaa aatcatactg acttcagatg
    34081 ctcacatttg ttttgacata ggtaactatg agtattctga aaatatctta aaggaaatta
    34141 attttccaga tgaattaata atgaattatc ctaaaaaact tattaatcat tttcaccaaa
    34201 aaggtaaatt actagatgtt gattatacaa atatctaagt aataatacat gcttacttta
    34261 actcccataa aaattttaat actatttata ttatatgaaa taaagaaatt ataataaaga
    34321 agctatttaa taaagcttaa ttacttttat gaacttgatg gattgatatg gtctatgata
    34381 gcaatagaaa ttataacatt tttaataaac ttagttttat ataccttata gtataaatct
    34441 aaaaaagcat catctaaaaa ggagctgtca catgagcaaa ttaaatttgt tctatgatag
    34501 ctcttttttt acttatttta attaggtata tttttaatta ttttatataa agcttatgtt
    34561 ctaagtaaaa ttatgcataa caaacaaagg tataaaaata ccattttttc agataatatt
    34621 taat
//
LOCUS       DBGJFU010000030        33880 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.030, whole genome shotgun sequence.
ACCESSION   DBGJFU010000030 DBGJFU010000000
VERSION     DBGJFU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.030"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..676
                     /locus_tag="ACOIAS_001408"
     CDS             <1..676
                     /locus_tag="ACOIAS_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701124.1"
                     /translation="FLINPEDAFDYIIFYIFILLIICVAIAAPTFSGEYQTQSDSILR
                     CTKHGHIRLAFTKILALFSIFIIMFVICISIHLAISDLAFGTECLKTSFQMLFSVISL
                     VNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSW
                     IGYLLPPGGIGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTY
                     CKHQVP"
     gene            1057..1974
                     /gene="pstC"
                     /locus_tag="ACOIAS_001409"
     CDS             1057..1974
                     /gene="pstC"
                     /locus_tag="ACOIAS_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGZ2701125.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQGIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            1976..2800
                     /gene="pstA"
                     /locus_tag="ACOIAS_001410"
     CDS             1976..2800
                     /gene="pstA"
                     /locus_tag="ACOIAS_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGZ2701126.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2819..3583
                     /gene="pstB"
                     /locus_tag="ACOIAS_001411"
     CDS             2819..3583
                     /gene="pstB"
                     /locus_tag="ACOIAS_001411"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGZ2701127.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            3611..4264
                     /gene="phoU"
                     /locus_tag="ACOIAS_001412"
     CDS             3611..4264
                     /gene="phoU"
                     /locus_tag="ACOIAS_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGZ2701128.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            4422..5291
                     /locus_tag="ACOIAS_001413"
     CDS             4422..5291
                     /locus_tag="ACOIAS_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGZ2701129.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            5378..6814
                     /locus_tag="ACOIAS_001414"
     CDS             5378..6814
                     /locus_tag="ACOIAS_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HGZ2701130.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6862..7689
                     /locus_tag="ACOIAS_001415"
     CDS             6862..7689
                     /locus_tag="ACOIAS_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2701131.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    7985..8247
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8323..10989
                     /locus_tag="ACOIAS_001416"
     CDS             8323..10989
                     /locus_tag="ACOIAS_001416"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGZ2701132.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            11414..12124
                     /gene="rgaR"
                     /locus_tag="ACOIAS_001417"
     CDS             11414..12124
                     /gene="rgaR"
                     /locus_tag="ACOIAS_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HGZ2701133.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            13188..14201
                     /locus_tag="ACOIAS_001418"
     CDS             13188..14201
                     /locus_tag="ACOIAS_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGZ2701134.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCLDIEENN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
     gene            14334..15632
                     /locus_tag="ACOIAS_001419"
     CDS             14334..15632
                     /locus_tag="ACOIAS_001419"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGZ2701135.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(15746..15991)
                     /locus_tag="ACOIAS_001420"
     CDS             complement(15746..15991)
                     /locus_tag="ACOIAS_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HGZ2701136.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            16139..17005
                     /locus_tag="ACOIAS_001421"
     CDS             16139..17005
                     /locus_tag="ACOIAS_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HGZ2701137.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(17009..17545)
                     /locus_tag="ACOIAS_001422"
     CDS             complement(17009..17545)
                     /locus_tag="ACOIAS_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HGZ2701138.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(17736..17939)
                     /locus_tag="ACOIAS_001423"
     CDS             complement(17736..17939)
                     /locus_tag="ACOIAS_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HGZ2701139.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            18230..18994
                     /locus_tag="ACOIAS_001424"
     CDS             18230..18994
                     /locus_tag="ACOIAS_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2701140.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            19383..20696
                     /gene="prdC"
                     /locus_tag="ACOIAS_001425"
     CDS             19383..20696
                     /gene="prdC"
                     /locus_tag="ACOIAS_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HGZ2701141.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      21100..21185
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            21484..21612
                     /locus_tag="ACOIAS_001426"
     CDS             21484..21612
                     /locus_tag="ACOIAS_001426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701142.1"
                     /translation="MDNQQVSFEKKKLQRLERNLVPTSVGKWGVPNLWLGNDIVCA"
     gene            21767..24016
                     /locus_tag="ACOIAS_001427"
     CDS             21767..24016
                     /locus_tag="ACOIAS_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HGZ2701143.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTSDVEKPSDGQVPDGEKPSDSQVPDVEKPSDGQVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQ
                     TPDVEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINK
                     IVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYH
                     DLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSP
                     YLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWI
                     ILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAG
                     REELKPNNTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDG
                     DGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKD
                     HSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
     gene            24423..26183
                     /gene="prdR"
                     /locus_tag="ACOIAS_001428"
     CDS             24423..26183
                     /gene="prdR"
                     /locus_tag="ACOIAS_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HGZ2701144.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            26612..28492
                     /gene="prdA"
                     /locus_tag="ACOIAS_001429"
     CDS             26612..28492
                     /gene="prdA"
                     /locus_tag="ACOIAS_001429"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HGZ2701145.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            28573..28857
                     /locus_tag="ACOIAS_001430"
     CDS             28573..28857
                     /locus_tag="ACOIAS_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBO2463/CBO2479 domain-containing protein"
                     /protein_id="HGZ2701146.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            28895..29620
                     /gene="prdB"
                     /locus_tag="ACOIAS_001431"
     CDS             28895..29620
                     /gene="prdB"
                     /locus_tag="ACOIAS_001431"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:29345..29347,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HGZ2701147.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            29700..30458
                     /gene="prdD"
                     /locus_tag="ACOIAS_001432"
     CDS             29700..30458
                     /gene="prdD"
                     /locus_tag="ACOIAS_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HGZ2701148.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            30473..30940
                     /locus_tag="ACOIAS_001433"
     CDS             30473..30940
                     /locus_tag="ACOIAS_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HGZ2701149.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            30967..31296
                     /locus_tag="ACOIAS_001434"
     CDS             30967..31296
                     /locus_tag="ACOIAS_001434"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HGZ2701150.1"
                     /translation="MGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSEHNE
                     WKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIMGAD
                     GLVITNP"
     gene            31297..31440
                     /locus_tag="ACOIAS_001435"
     CDS             31297..31440
                     /locus_tag="ACOIAS_001435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701151.1"
                     /translation="MDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRK
                     DAK"
     gene            31460..32467
                     /locus_tag="ACOIAS_001436"
     CDS             31460..32467
                     /locus_tag="ACOIAS_001436"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HGZ2701152.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            32587..33468
                     /locus_tag="ACOIAS_001437"
     CDS             32587..33468
                     /locus_tag="ACOIAS_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HGZ2701153.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT        13469 a         3762 c         6206 g        10443 t
ORIGIN      
        1 tttcttaata aatccagaag atgctttcga ttatataata ttttatattt ttatattatt
       61 aattatatgt gttgcaatcg ctgcacctac tttttctggt gagtatcaga cacaatcaga
      121 tagcatcctt cgttgtacga aacatggaca tattcgtcta gcgtttacta aaatattagc
      181 tctattttct atatttataa taatgtttgt tatatgtatt tctatccatt tggcaatttc
      241 tgatttagct tttggtactg aatgtttgaa aacctcattt caaatgttgt tttctgtcat
      301 tagtctagta aatataaatt tactacaagc acagattctt atagctattg gtggtctaat
      361 ttcaatactt gcaatggtta gtttaacatt atttttatct gcaaaatgta aggattctct
      421 tacttctatg ctgattgctt tttctatttg cttaattcct atgggaatag gtgttagttg
      481 gattggatac ctattaccac cagggggaat tggttttaag attagtttat tatatcaaat
      541 gacatcattt aatttcttac acttaggagg tatgagtttt tggacaccac acattatact
      601 gtttttctca gtgatagaaa tacctatttt tatatttttg gctattcgta cctattgcaa
      661 acatcaagta ccgtaacaat ttgatggaaa taatagtgat ataaactaga ggatacaata
      721 attatatatg tagtataaaa gttaaattta gttaacaaat cttaatagca gatataggta
      781 ataaattata cataaactgt attattatct atatctgcta ttttaatata gagaaacaac
      841 agtatattgt aaagtaaatg tcaaatatag ttttaaaagt aaaattaatg ttaaaaagta
      901 aatataattt tacacttgct taacttaatc ctaatataga tttaacttaa aaaaagtaga
      961 ataaaagtat aggaaatttg atgaagacga attataaaaa attaaatttt gaatacagaa
     1021 cgaaaatcga taagtatatt ttaatgggag ggtattatga tagtaaaatc tagattagta
     1081 ggtttagaaa aaaatgacaa taatggcaat aagacaaaat acgtaattga aaaagtagca
     1141 aaaaatattt ttctacttag tgctttagta gcagttgcaa gtttactttt gataatagga
     1201 ttcgtatttt ataaaggatt aagaccattt atatttgaag gatattcatt tatagacttt
     1261 ttaactggtg cagattgggt tccttctgcc aataaatttg gaatatcatc tatgatagta
     1321 gcatcaatat ttgcaacact tggagcactt gttataggag tacctgttgg tatattaacc
     1381 tctgtattta tagcagaagt agcacctaaa agattagcaa aaataatgtc accagcagtt
     1441 gaacttttag caggtatacc ttcagtatta tatggtgtat ttggtcttgc agtgatagtt
     1501 ccaactatac aaggtatatt taatcttcca aaaggtcaaa gtttgttagc aatagtaata
     1561 gtattagcag taatgatgtt accaacaata atatctgtat cagaaacagc aataagagca
     1621 gtaccaaaag catacaaaga aggttcatta gcacttggtg catcaaaaat agaaacaata
     1681 ttcaaagttg tattacctgc tgcaaaatct ggagtattag ccgcagtagt gttaggagta
     1741 ggaagagctt taggtgaaac aatggcagta atacttgttg caggcaactc accagttatg
     1801 ccaactgcaa taacagatag tgtaagacca cttacaacta atatagcact tgaaatggga
     1861 tacgcatttg gtacacatca agaaatgcta tttgcaactg gagtagtatt gtttacattt
     1921 atattaattt taaacttagt tttaaataaa ctttcaaata aggcggtaaa ttaagatgag
     1981 aaagttcaaa gagaatctat tgaagtcatt agtatatcta tcagcactat ttacaatagt
     2041 atcattagta gttatagtgg gatttatatt tataaaggga attggcaaca tgaattttaa
     2101 tttcttattt agcaattatt cagcatcagg tgatggtggt atacttccaa tgataattac
     2161 aacattatat acagtagtag tttctatagt tgtagcaact cctataggaa tattggctgc
     2221 tatatattta caagaatatg ctaaaaaagg aaaagcagtt acagctataa gatttgcaac
     2281 tgaaagttta tcagggatac catctatagt atatggatta tttggaggaa ttttctttgt
     2341 agttacatta aaaatgggat attccatagt tgcaggttca cttacagttg caataataat
     2401 attaccagta ataattcgta caacagaaga agctttaaaa acagttcctc aagcttatag
     2461 agaatcatca ttggcattag gagctacaaa atttcaaact ttatataagg tagtacttcc
     2521 aagtgcaata ccaggaattt tatctggagt aatcttatca gttggtcgta taattggaga
     2581 atcagctgca atattactta cagcaggtac tgtagccaag atgccaggtg gaatatttga
     2641 tagtgcaaga acattaactg tacactcata cttactgact aaagagagtg gagatatagc
     2701 aacagcagcc agtataggaa ttgttttaat agtaattgta ttagcactaa acatgctagc
     2761 aagatttgtt gctaagaaat taaataaagc taattattaa atttaagagg tgtgaataat
     2821 ggaattaatc gataagataa aaatgagtgt aaaagatttg gatttatttt atggtgataa
     2881 gcaagcatta aagaaaataa atatggatat aaaagagaat aaggttactg ctttaatagg
     2941 tccatctgga tgcggaaaat caacttttat aagaacgtta aatagaatga atgacttaat
     3001 cgaagatgta acaattaaag gaaacatttc tgttgatgga gaggacatat atacctcaga
     3061 tgatgttata aatcttcgta ctaaagtagg aatggtattt caaaagccaa atccatttcc
     3121 tatgagtata tatgataatg tggcatatgg tcctcgtaca catggactaa gagataagaa
     3181 gcaattagat aaaattgtag aagaaagctt aaaaggtgca gctatttggg atgaggttaa
     3241 agacaggctt aaatcatctg cactaggact ttctggtggt caacaacagc gtatatgtat
     3301 agcaagagca attgctatga gaccagaagt tattcttatg gatgaaccta catctgcttt
     3361 agacccaatt tcaacattaa aagtagagga acttatagag gatttaaaaa aagactacac
     3421 aatagtcata gttacacata atatgcaaca agctgctcgt atatcagatg aaacagcttt
     3481 tttcttaaat ggagaggtta tagaatttag cgatacaaaa actatgttta ccactcctgt
     3541 agataaaaga acagaagact atataacagg tagatttggt taaaatatat aactataaag
     3601 gagttagaca atggtcaata atagtttaga tataagtatt gatactttaa aagaacaaac
     3661 agtgcttatg atggaaaaat gtgaagaagc aatagaaaag tctgtgactt gtatgataga
     3721 gaaggattta gagggtgcaa gaaaagtagt agaagatgat gaagaaataa ataaattaag
     3781 agaagaaata agagataaga gtatagagct aatagtatta aagcaaccaa tggcaaggga
     3841 cttaagattt atttattcat taggtaatat atctatagag ctggaaagaa taggagatta
     3901 tgcagcaaat atagctagag aaatgttgaa gataggtgga gaggcttata taaaagagct
     3961 aatagatatt cctaagatgg caaaagaatg taagtctatg atgtctcaga ctagagaagc
     4021 tttaatctta tttgatgcaa gtatagctta tgatgttgga actaaagatg atattgtaga
     4081 tggattgtat gaacaagtac aaattgattg tttaaaaata atgcatgaaa actcaaatac
     4141 catagaccaa ggggtaaaac ttctacttat aggaagatac cttgaaagaa ttggagacca
     4201 tataacaaat gtatgtgaga agataatttt tgcaattgag ggtaaaatgg ttgagatagg
     4261 ataaaaaact gctttaaagc agttttttta ttgtctaaat taagaaagaa ttgataaaaa
     4321 aatatgatta atgggtaaat ttaaaaaatt gtgatatact aggaacatat taaatatgag
     4381 gatgcgtatt ttatataagg tgctttttag gaggtggaac tttgaaactt caaaagatgg
     4441 gtatgaggac tataaaaaca ggaattagtg tcactttatg tatgattctt ggtgactatt
     4501 tagtagagaa tatgttttat tctctggtag catgtataat ttctgttcaa gatacagtaa
     4561 aaggttcttt aaagacggga atcaatagag tggaaggtac tattttaggt ggtataatag
     4621 gattcatatt tgcaatcatt aaagctggag acccattttt atgtggtcta ggagtaatgt
     4681 gctcaatata tttatgcaat ctgtttaaaa taaagtcagt agtagttgcc tgtgtaacct
     4741 ttctggctat acatctaggt gtaggaaata gtgaccctgt gtattattct ttacataggg
     4801 tgttagatac atctgtgggt gttttgtttg gagtacttgt taattattat gttgctagac
     4861 caaattatct aaatagtgtt atagatgagt ttaaaaagat agaagaactt tcaatgttgc
     4921 ttattgaaga taaaattctt agaaaagaaa atttaaatgt agagaaattt gaagaagaag
     4981 tagaaaaatt agaagaaata tattcaaaac ttattgatga gttggattat agtttgagtg
     5041 aaactaatat agaaaaaatt gagaatgcct taaggatatg tagagaaata cattttcata
     5101 tgcagtctat agagttactt gaaaataaac tttatttgaa agagcataca tataaatacc
     5161 ttaagaaaac atataatgtg gataagctag actggaatat agatgaagaa aaaagccctg
     5221 tattcaacta tcatctaatg aaaataatga ttgaaataga gttgttgaga aagctaaata
     5281 aatcatatta ataatagtag ttaaataata aataaaaaat agtatctttg taaatattaa
     5341 acatatataa gtttaattta tggaggtact attttttatg aatactagaa atgacaattc
     5401 taaaatacta gaaatatata aaatgaagct ttttggcgaa cttgtaaaag cagtgtggaa
     5461 tgatgatata gaggatattg aaaacttacc taataaaatt ttaaatgaag atttaaagga
     5521 taatttaaat aattatttta gtggaaaatt tgataaagat actatggtaa atttaattag
     5581 ggtaactatg ggattggatg ttacagaaga tacagataaa aatttaaaag agtcactgca
     5641 tgaagctgta aatttagatg aagtcaaaag accaataata tctgtaatat cagatgcttg
     5701 caaatattgt gatgaaaata ataaggaaga tgagtcatgt aagataagaa gtaagcatat
     5761 taattgtaat gaggaagatg tatgtagtgc ttgtggtgat tgtgtatcaa gatgtaagtt
     5821 gggtgcaatt tcagataaaa tagaatttat accaatgata aaattgttaa aagatataaa
     5881 gagccctgta tatgcaatag ttgcaccagc ttttgtagga cagtttggga aagatgtaag
     5941 ccctggtaaa ttaagaactg cattaaaaaa gataggattt gaagatatga tagaagttgc
     6001 tttggcagca gatatgctta ctgcaaaaga agcatatgat tattatgagc atatgaaaaa
     6061 ggacgaagaa ggatgcttta taactagttg ttgctgtcct atttgggtta gtttgataca
     6121 aaatagtttt cctcagatat tggaaaatgt atcaccatcc gtttctccta tgattgcttg
     6181 tgggagagct gtaaaaattt taaatccaga tgctaaagtt gtattcatag gtccttgtat
     6241 ggcaaaaaag aaagaagcaa tgttagaaga tataaaagat gcagttgatt ttgtgcttac
     6301 atttaaagag ttgcaagaaa tatttgatgc attagagtta aatccagcaa taatggatga
     6361 gagtaataga gtagaggcgt ccctttcagg tagagtttat gctagaacag gaggtgtaag
     6421 taaagcagtt aagttatcag ttagaagaat tgacaaaaat ataaaatttg aagaaaaggc
     6481 atttgaagga acaaaagagt gcatggacgg tctaaagaaa gttctaaata aagaaataga
     6541 agctacattt atagaaggaa tggggtgtgt aggaggctgt gtaggaggtc cgaagcgtat
     6601 tttaagtgtg gaagaaggta ctaagaatgt tgatgaatat agtgaaagta cgaatatgga
     6661 aactccattt gataatttaa atgtattaca atttttaact tctttgggaa tcaaaagagt
     6721 agaatcttta ggagaaaaag aagaagaaaa ggttaaaaaa atatttagta gagatataaa
     6781 aagtaataag aaagagaata aatataaaaa ataacttttt aagtcttaaa cttatgcttt
     6841 tataaaaggg agatatgggg gatgaaaatt aaagataata aggctgtttt tcatgtattt
     6901 ataaggatat ttcttatagc tgttttaatt ttaactggaa cattattgga ggaaaaaatt
     6961 aataatgttc cagttgaaaa tataagttca aaaggtgaaa atacactgga actgctttct
     7021 caaagtaata ctttaggaga aatcatatac aaagcatctc aagaagaaac aaaaggtcca
     7081 ggggctactt ctgaaaaagt agcatacata acaatagatg atggtccatc aaaatataca
     7141 aatgcactac ttgatatttt aaaagaaaat gatgtcaaag cgacattttt catgcttaat
     7201 cagaatatga aaaaccacaa ggaagaagta aggagagtct tggaagaaga aaactcaata
     7261 ggttttcata gtgtaagtca tgatatacat aagttatata aaagcccaga agtaacagtt
     7321 ggggaattta atacatgcaa taatactcta caagaaataa ctggtcagag ttcaaaatta
     7381 ataagattac catacggaag taaaccatat atgccagaag gttcatataa taaacttatg
     7441 gcaaatgatt atttaatttg ggactggaat ttggatacac aggattggaa gtcttctaca
     7501 agtcagatag taagtaatat attatattat ggaagaaaaa gagatgaatt agtagtatta
     7561 attcatgaga aagagcaaag tttaaatgct ttaaataata ttataagaat cttaaaagag
     7621 agaggatata caatattgcc aattacagaa aatataacac ctaaaaattt ctggcaagaa
     7681 aacttataat aaatttaaat atagtaaaag ataaagtaaa ttatttataa aataaaacat
     7741 ataatataaa aaatacgaat aagaggtata ctgtaaggat aatataaaat taatgagaaa
     7801 gcattaaaat taaaataaaa taatcttgca attattgaca ttataagaca gaataactgt
     7861 aaaaacatat tttaaaatat aacaactata taaaagagaa tagacataat taagaaaatt
     7921 cagaaaaaat aaaataaaaa taattgacaa agagaataat atttgtatat aataatatgt
     7981 aatattaaag actgagaaga agaggagtaa gtaaatacta ctaaaagaga ggaaaattca
     8041 ctggctgtaa gattttctaa gtataagatt tactgaatgt agctttggag tttctaaact
     8101 gaactataag taagtttaga cggttagaac cgttaaattc tttgagagtc aaagttgata
     8161 aaataacatt aaccaataat ttaattatat gttaatgtat tttaagcttt ggaattaagg
     8221 tggtaacgcg agcttttcgt cctttttaaa gaggatgaag agctcttttt tatttctaaa
     8281 gatatttaaa aaatttattt ataaaataca ggaggcagga caatggaaaa tacgaattta
     8341 tcaaaaactt ataacccaaa agattttgag gctaggctat ataaaaaatg gatggatgaa
     8401 gggtatttta aatctaaacc aaatccagat aaaaaaccat ttacaataat gatgccacca
     8461 ccaaatataa cagggcaatt gcatatggga cacgctcttg accatacact tcaagatata
     8521 ttaatcagat ggaaacgtat ggatggatat gaagcttttt ggcttccagg aactgaccat
     8581 gcttctatag caacagaagt taaagttgtt gaaagaataa aaaaacaaga aggcaagaca
     8641 aagtatgaaa taggtagaga agaattttta aagagagcct gggaatggaa agatgaattt
     8701 ggaggaaaaa tatctaatca gcttaaacaa ctaggtgatt cttgtgattg ggataaagaa
     8761 agatttacta tggatgaagg gtgtaatgaa gcagttatag aattttttgt aagcctatat
     8821 gaaaaaggtc acatatacag aggaaataga ataataaact ggtgcccaga ttgcaaaact
     8881 actttatcag atgcagaagt tgaacatgaa gaacacgacg gaaactttta tcatataaag
     8941 tacccactaa aagatagtga agacttttta gaaatagcta caactagacc tgagacaatg
     9001 ataggagata ctggtatagc agtaaatcca gaagatgata gatataaaca tttaatagga
     9061 aaaacagcaa tacttccatt agtaggaaga gaacttccaa tagttgcaga tagttatgtt
     9121 gatttagagt ttggaactgg tgcagttaag atgactccag cacatgaccc taatgatttt
     9181 gaagtaggat taagacataa tcttgagcag ttaaatacta tgaatgaaga tggaactatg
     9241 aatgaagttt gtggaaaata tgaaggtatg gatagatttg agtgtagaaa agctatcgta
     9301 gcagacttaa aagaacaagg ttacctaatt aagataaaag agcataatca taatgtagga
     9361 acttgttata gatgccatac agtagttgag cctagattat cagagcaatg gtttgttaag
     9421 atggaagaac ttgcaaaacc agctatagat attcttaaaa aaggagaatt agaatttgtt
     9481 ccagataaat ttgataaaac ttatttacaa tggctagaaa atataagaga ttggtgtata
     9541 tcaagacaat tatggtgggg tcatcaaata ccagcttatt attgtcaaga gtgtggagaa
     9601 atagtagttg ctagaaaaat gccagataag tgtcctaaat gtggaagcac tcattttaaa
     9661 caagatgaag atgccttaga tacttggttc agttctgcat tatggccttt ctctactcta
     9721 ggatggccaa ataagactga agctttagat tattattatc caacaagtgt acttgttaca
     9781 ggatacgata taatattctt ctgggtagtt agaatggcat ttgcaggtat gttctgtatg
     9841 aatgaaaagc catttgacca tgttttggtt catggattag taagagattc tcaaggtaga
     9901 aaaatgagta aatctttagg aaatggtatc gacccattgg agataatcga gcaatatgga
     9961 gctgatgccc taagatttac attaacaact ggaaattccc caggaaatga tatgagattc
    10021 tatatggaaa gagtagaatt tgctagaaac tttgcaaata agttgtggaa tgcatcaaga
    10081 tttgtattta tgaatattga tgaagatata attaaaaaca tgactagaga aagtgttaaa
    10141 gaggacttaa ctctagctga taaatggata atatcaagag caaataatat tgtaaaagaa
    10201 gcaactaata acatggataa gtttgattta ggaatagcac ttcaaaagat atacgatttt
    10261 acttggtcag aatactgtga ctggtatatt gaaatggtta aacctagatt atatggggaa
    10321 gatgcaaatg ctaaatctgc agcattatat acattaactt atgttcttga aaagatatta
    10381 aaattattac atccatatat gccatttata acagaggaaa tatatactca tcttcctact
    10441 gttgaaggat gtataatagt atctgaatgg ccaaaatata atgaagaaga taacatggct
    10501 gaagaagaag atatgatgaa cttattgatg gaaggtataa gaagtataag aaatgtgaga
    10561 gcagaaatga atgtaccacc ttctaagaaa gctaaattga taataatccc aagtgaagaa
    10621 aaaatagaag ctattgagct tggtaaagac tattttataa ctttagcatc tgcttcaaat
    10681 gtagagatag caaaagataa atctaatgtt ccagaagatg cagttggagt agttattgat
    10741 ggtgttgaaa tatttatacc tcttaatgaa ttagttgatt ttgaaaaaga aattgagaga
    10801 ttatctaaag aaaagaaaaa gttagaaggc gaaataaaga gagttaatgg aaagcttgca
    10861 aatcaaggtt tcttagcaaa agctcctgaa agtttaattg aagaagaaaa agcaaaaaaa
    10921 gaaaaatttg aagagatgat taaatctgtt gaggaaagat taacaaattt agaatctaaa
    10981 ataaaataaa tataagaatt agtaaaaaca tacaaaaagt atatattcta tactttttgt
    11041 atgtttttct ttatatatat acaaatatga tgttataatt ctttacagat gtgttatatt
    11101 tttattttat gttgtgaata tatgaatttt atgtcttaaa atttataagc aataaaaatt
    11161 tgatatatta tgatgtttaa tatagggtaa taaatacata ttatatttat tttactatat
    11221 ttttttacat aatatatcta gaaaagtata ggaatttatc gtaatttaag tttaaatatt
    11281 aaagagaatt tctataagta gtttaatcga caatttaaat ttataaagaa atttaaattt
    11341 gaaagctagt gagtacagtt tggaaattga aataaagtaa actaaatgat attttagatt
    11401 tggaggtgat attatgttca gggtagttat atgtgatgac gaaaaaatac aaagaagcat
    11461 actaaaagag tttacagaaa aacttttgtc tgaaagatgt ctaaattatc aaatactgga
    11521 attttcctgt ggagaagatt taatatccaa atacccagaa aaaattgata ttatattttt
    11581 agatatccaa atgaaagata tcaatggaat tgaggtggct aaaaaaataa gaaagtttga
    11641 tgaaaaagta gaaattatat ttacaacggc tttttcagag tatgcccccc aaggttatga
    11701 agttcgtgct tatagatatt tagtaaagcc tatagagtat agtaactttg caatgggtgt
    11761 aaatctttgt attgataacc ttcaaagaaa gaaggagcgt tatatagttt taaatagtaa
    11821 aaaaggattt aatagaatac tgatagattc tattttatat gttgaaactg taaagagaga
    11881 tttagttgta catacaatag gtagagatta taaagctaat atgagcatga aacaagcaga
    11941 aaaattactg tgtgaaaatg gcttttttag atgtcataca agttttattg ttaatttaag
    12001 aagaatagaa gatcttaggg ataatacgat tactattaat gaacaattta tacctataag
    12061 taagtataga cttaaagatt taaaagtagc attaacaagc ttgttatgtg atataatgta
    12121 ttaattttaa aaaggtaatt gaaaatatcg taaaaaagat ttaatgtcat cacagggcta
    12181 ttgattgaaa ctgtcttaat atataaaatg ttatttatat taagacagtt ttattatgca
    12241 aaatagcagt ttatagactt ttaatatggt ttaatatgta tatgtagagt ttccattata
    12301 atagcatgat tattaattag tagcttgatt atcaatatac tattattgat ttttatatgt
    12361 attatatgta tttttagaat atgattacct ttttaaaata atgactattt ataaaactct
    12421 atagatattt gtttcattta gctatgcata ctttaattaa ttatattaat atttaatgga
    12481 gtacaatata aatattaaag cgacatctta tagtttattg aacctatagt aatgaattaa
    12541 taaatagttt aattctatga aaagatttcc ttttatgcaa tttatttatt gaaatatgat
    12601 ataatatata aaggtgtaag tattagttgt taaaataata tttatattag tttttatgca
    12661 tacttggtat aagtttttca tatattgatt aaaattgatg ttgatttatt atttttagta
    12721 ttttttcacc ttttgattca tactttgtag aatggtgtat tttctaaaac tgattataag
    12781 acaagttcat tcaaattagc atttttatat catataaata attgttcccc ctaaataaga
    12841 tttagatgta tagtattaaa atcaaaaagt ataaaataaa attgatgctt aatcatagat
    12901 aaatattaat aaaatataca aggggattgt ctaataaaaa gataatctcc ttttttacaa
    12961 ataattctaa attgtgatat aaatctaaaa attttatctt aacatttaac catttaatga
    13021 taataattga tataataaat ataaattaaa tatatggtgt acttatttta tgtttttagc
    13081 aagaaatata gcatttaaaa gataaattgt gcaacttaaa tcaaagttta agatgaacta
    13141 ttaacataga tatattaaaa acttttgtaa tggagaatat atgtgatatg aataataaag
    13201 ttaaggttgc agtattagat acaggaatag acaaagaaca tgattactta aaagataatt
    13261 tagttggagg aattgctttt gaatgtatgc atgattatat ctttatatca gataaatttg
    13321 atgatgaaga tggtcatgga actgcttgtg catcaataat aaagaaagag tatgaagatg
    13381 tagagttatt tgtaataaaa attttgagaa aagatggtat tacaaatata aaagttcttg
    13441 aagaagcatt aagatacctt ttagacacta acataagatt aattaattta agtttatcag
    13501 tcataggagt agaaagtgtt aagggcttat ttgaagtttg ttatgaattg tttagaaaag
    13561 gaaaaataat agtatgttca ctcgctaatg attttgactt aagttatcca gctatgttta
    13621 acaatgttat aggtgtaaga ggatttactt tagatggaga tgattcattt tggtacaaca
    13681 aaaaatatga tgttcaatgt gttatggata gcaattcata tataagttgt gatataaata
    13741 attcctatag attaccacca aaatgtaata gttatttatc agcaaagctt actggtaaaa
    13801 tagctaagat attatcagaa aaaccaggca taacaatatc tgatttaaat gataagcttg
    13861 aatcattagc aactagaaat cattgggata gttgtgattt tgatgagtgt agtagaattg
    13921 taaactttaa attggatttg tatgacaaag aaaatgctct ttttatgaaa gttgctgacg
    13981 ttgttaggga ttgcttagat attgaagaga ataatgaaaa actctttaag ggtagccttt
    14041 ttaataagga gataggcttg gtttatgata attgttttaa tctgttgaaa aagctagagc
    14101 atagatttga cataaagttt aattatatgg acatctcaaa atatgactta gtttcaatat
    14161 atacactgac tgaattagtg gaaagatata aaaacttata aataaagatt tgtagctaaa
    14221 tttaatgtag aaggtattaa attatatgta ttctttgtcg gtagattaaa agtaaaaaat
    14281 atactataat tagatagcat aagagaaaat gccaatacag ggggagttag ataatgaaat
    14341 atgaagaagc attagagtac atatcacaaa ctaataaatt cggtataaga ttaggattag
    14401 aaaatatcgg aaagttatta gaattattag gaaatccaca agaaactttg aatataatac
    14461 atgtagctgg aactaatgga aaaggttctg tgtgttcttt tgtttctaat atactaagag
    14521 agtgtggata taaagttggt ttatatacat ctccatattt agaaactttt actgagcgta
    14581 taagagtaaa tggacaaaat ataccacagg atgatgttgc tagaataatt gagttaataa
    14641 aagaaaaaat agagattatg gtaaaagaag gctatgcata tccaacagaa tttgaagtgg
    14701 ttactgcaat ggcattttat tactattcag aacaaaaggt agactttgtg gcattagaag
    14761 ttggtttagg tggcagatat gatgctacaa atataataac aaaatcactt gtaagtgtga
    14821 tagtatctat aagtttggac catacaggta tacttggaga tacaatagaa aaaatagcgt
    14881 acgaaaaggc tggaataata aaggaaaatg gagtagtact agtatatgac cagactgatg
    14941 aagcaaagga tgttataaag tcagtttgta aagagaaaaa agctaaatat atagaagttg
    15001 attttgatga tataaacata aaaaaatcag atataaattc tcaaatatac gattgtactg
    15061 taatgaaaga aacttataga gaccttgaaa taaaattgat aggcgaacat caaataaaca
    15121 attctatctt ggcaattagt gtaataaaat atctaaaaga cataaataaa ttagcaaata
    15181 taaatgaaga atctataaga aaaggactta taaatactaa atggccagga agaatagaaa
    15241 agataaaaga aaatccaatt tttataatag atggtgcaca taatgaagat ggagcaaaat
    15301 ctttggcaaa agcacttgat aagaatttta aaggtagaaa attaacctta ttaataggta
    15361 tgttagaaga taaagatatt gatagtgttt tagagatatt gttgccacat tttaataagg
    15421 taataacaac aacaccaagt aacccaagag ccataaattc agatatatta agagaaaaag
    15481 tattaaaata tgtagatgat gtaacatcaa agcatgagat agaggatgca gtaaattata
    15541 cattagaaac atcaagtgag gatgatatta taataagtgc aggttcatta tatatgattg
    15601 gtacagtgag aactttagta aagaaattat aattttataa aataagtata caaataaaaa
    15661 tataaaaaag agttgtctaa aaaatggaca actcttttta tataaaataa gtttttaata
    15721 attttactga tgtttatata agtaattata tagattgacg tatagcttta gataattggt
    15781 ctgggcaaga agtacctttt ccaccacagt caataccatt taatttatca gcaacttcaa
    15841 gagcattttg gccaattatt aagcttctaa gtcctgataa attaccatta catcctccta
    15901 taaattcagc atcaacaatc acattatttt catcaacttc aaaaatcatt tctctacaac
    15961 atacaccact tggattaaat gttactttca ttaaaaagac ctccattaaa tattatttct
    16021 ttttttaaca ttaataataa taccaaatat atgcgtaata atgcaactat aaagcataat
    16081 acaagctaat gaaataatat attattgtag tatatgtatt gtagagagga ggggttatat
    16141 gaaaatctat atttctaact acttatcaaa gagtattagc atagttgatt attctacact
    16201 tgaactagaa aaagaaatag tattagaaga taatatatat ccacaccatt tttgcataga
    16261 aaaagaaaaa aatttaatat acatacctag ttcaagtaat gggatactct atgtcttaga
    16321 tttaagtaat gataaaataa ttgatactgt ctctatagga ggaagtctta gtcaagttgt
    16381 acttagcgat aatgaattgt ttgtggcaaa tgaagattct aatagtatat atgtactaga
    16441 taaaaacaca ttaaatccaa taggaattat tggagttgat aatatgccac atggatttga
    16501 ctttgataga gaaagcaaaa agttatatgt accgtgtata aactcgatag tatgtataga
    16561 tacaattaat aaaaatatac aaaagaaaat aaatatggac tttagagctt ggcatataga
    16621 acttgataaa caaaagaaag aaatgtatat atcaacactt gatggaaagc ttataataat
    16681 agatgaagta agcatggata taaaaaaggt attatatgaa tttttattac cagtagaaat
    16741 aagatttaat tatagtggca aaaaagttta tgttgctgat ttgggttata ataatgtaag
    16801 aattttagat tatacaacga gtagatacat tggaaatatt gaaataaatg ggattcccca
    16861 aggattagaa atatctaaag atgaaaagtt actatttgta tcagatacac aagaaaattc
    16921 agtaaaagtt tatgaaactg caaataacaa attaataaaa gaaatcaagg taggaaaaga
    16981 gcctacaact ataatttgtt tgtaggcttt aatgtatgtc tcttataagt aaatcgataa
    17041 cttcggcata catataagat gcattatttt tccagttatt gcaaataagt tttgcttgtt
    17101 tctctgatga aacatttaga tttaaatcta ttaagttaga ctgattttct atgactctaa
    17161 ggttgacaat aaattcatta tcactttgtt ttacaaaact agactttact tgagtatctg
    17221 ctagtagatt ttctttgtta gaatctatat acttgtctat tttttcagta acactagtag
    17281 gtattcgatt aaagaagtat tctagacttt ctacgcctct ttgagtaaga gcataatatt
    17341 ctctgtcaga gtctttatat gtagtcaaaa acttagattc cataagctga gataaaagct
    17401 gttgtaatga aaaataattc atcatctcag tctctaatac tacttgagtt atttgagaat
    17461 tagttaaatc cattttaatt ttttctaata tatataaaat taaaagtttg tgataggcta
    17521 attcttcaga cgagttttca aacataaata tcatcctccg tcaaactatt tctatatata
    17581 atatattaaa ctaatttgta caaaattaaa agaacaattt gtattgtatt attaatcttt
    17641 aataattcat ataaaaacaa ttttataaat aaatatagaa aaacagggct gctaattagc
    17701 agccctgttg atacccaggt gtcaaaattt actatttatt ttccagccat ttgtctttca
    17761 gccatttcaa ctaatttctt agtcatatat ccaccaacat atccattttc tctagctgtt
    17821 aagttacctt tgtctatgtt ttcatagtta gataaaccta tttcgttagc tatttctaat
    17881 ttcatttggt ttaaagcagc ttttgcttct ggaactactg ttctgtttga atttgacata
    17941 gtaaatttcc tcctcaaata attagtttat gatgtctaac aacactttta tttatgtgat
    18001 gttaatacta atttctccaa aataaaaata atatatacag aaaaataaag gtaaaaatgt
    18061 aaaaaatata aaaaaattaa ataaatgagt attttagaga gtttaattag gtcaatatag
    18121 gttttatttt tttagttatt gtcttataaa ttaaatatta cagtactttt ttgagctgaa
    18181 aaaaaataaa ttttaaatat agaagaaatt atttcaaagg gggaatagta tgaaaagaga
    18241 agttaaaagg actaaagctt tgatatatag tgttttattt ttttcattat tattagtgtt
    18301 aatatttgtt tttgtttatg gaataaaata catatgttct aaatctgaca attcaaaaat
    18361 gcctatatat aaagttgata caaatgagaa aaaaatagct ttgagttttg atgtagcatg
    18421 gggaactgat aatatggatg atattttaaa aattttagat aaacataatg taaaagcgac
    18481 cttcttttta gttggtagtt gggtggatga taatgaagaa attgtaaaag ctatagatga
    18541 aaaaggacat gaaattggaa atcattcaaa tactcatgcc aatttaaagg aaatatcaaa
    18601 agaagacata acaaaagaga tagaaactac atcagaaaaa atatataata taacaggaaa
    18661 aaggactaat ttatttagac ctccttttgg ggacgtaaac aataaagcta tggacatttg
    18721 tagtgattta ggttataaag taattaagtg ggatgtagat tctatagact ggaaggagct
    18781 tggaccaaat catgtaattg aaaaagtaat aaaagaatct caacctggtt caatagtttt
    18841 attccatgct aatataaatg atgtagataa ttatttggat actataataa gtcgactaaa
    18901 aaaagatgga tacagtctag tcaaaatatc tgatttgttg tataaagata attatgtagt
    18961 tgattccaat ggagtgcaaa aaactaagaa ttaataagta ataaatgtat attttaagta
    19021 aagttgtgat aaattatata gtatatgtta gaaaagtgtc gataaaaaga cggtgtctat
    19081 tttttgacac tttttataca tgtatgcaat atcaataatt atagattaaa ttgattaaaa
    19141 aagtgtctaa taattgactt ttttttgatg ggtttaaaaa tttagaagaa ttaatgaatg
    19201 ctgtaagaca gtatttccaa agggtataag tttgtttaaa agttggcata gaaattgctt
    19261 tgttaataag cgatatgttc tatagaaata aacaatatgt taagacctta taatagaaaa
    19321 atagcattaa cattatttga acagcttata aaaaaattaa tatacttaag ggggcgtact
    19381 agttgaacaa actatattct attttattaa aacaacacgt tggtggtcca gataagccag
    19441 tagtaagtgt tggagatgta gtaaaaaaag gaactctaat cgcagaacca actggattag
    19501 gggcaaatat ttatgccagt gttagtggaa aaataagtga aataaatgac caagcgatag
    19561 taattgaagc tgatgaagta caagaagata cttttgaacc attaaaagga gaaggtatac
    19621 ttgacttaat taaagaagct ggagtagtag gaatgggtgg agcaggattc cctactcatg
    19681 taaaattaaa catagattta aatggtggaa caatattagc aaatgcagct gaatgtgaac
    19741 ctttattagc tcataatata aaagaaatag aagaaagacc agaaatagtt tatcaaggta
    19801 taaaatatgc tatggaagtt actaatgctg gaaaaggtat gctagcaata aaaagtaagc
    19861 atccaaaagc aatagaagct ttcaaaaaag taataaagcc aggagacaac atagaagttg
    19921 cagaactagt tgatatgtac ccaatgggag aagagagagc aatagtaaga gatgttctag
    19981 gaaaattact tgagccaact caattacctt cagaggctaa tgcagtagta ataaacgtag
    20041 aaacactaac tagaatagta gaggctgttg agcaaaaaag accagttata tctaaaaata
    20101 taactgtagt tggtcaatta aacagtggaa aagaatctat agtgtttgaa gatgtgccaa
    20161 ttggaacaac tgtcggagaa ttaattgaaa gagctggtgg aataaaaggt gaatatggtg
    20221 aaataatcct aggaggacct ttcactggta aagctactac tttagatgct ccaataacta
    20281 aaactagtgg tggtataata gttactatgc catttgttaa tgaaaagaga aaaatgggtc
    20341 ttttagtttg tgcatgtgga cctaatgaag aaagaatgag agatatagca actaaaatgg
    20401 gagttactga tatagtatca gttcaaaaat gtaaacaagc tcaagaaatt aaaggtgcat
    20461 taaaatgtga aaatccagga cactgtccag gacaagctca aaaatgtatt gaatttaaga
    20521 aagctggagc agaagttata ttaataggaa actgtactga ctgtagtaac acagtaatgg
    20581 gttctgcacc aaaattaaaa ttaggtgtat atcatattac tgaccatgta atgagaacag
    20641 ttaatcaccc attaataaga agaataaaag gtgacgtaag attaaaaata aaataataga
    20701 taaatagaag ttagacatct ttagaagtaa aacttaaaag ttacttctaa ggatgttttt
    20761 taatgtattt tgagggcaat atatatattg taaaataaat ggaaaaaata gacaaaatat
    20821 gctagatatt gtataatgat aacaatagct attacaatta atattggaca aaaataatga
    20881 tactcaagta tgtggtttgt attaatatgc atattatatt aagtcaacta tccgatagag
    20941 taaaaggttt ttgtgatatt tgaagatact ctatagtatc ttttttatta taaaaactaa
    21001 tttttgtctt ttattagata agtatctagg atactaattt atattttatt gagttgttaa
    21061 aaaagtgcac ttaaaaagtt aaaaaagaaa aattaaaaac ctatttatag aaactgtgaa
    21121 gtatatctta aacctgggca cttaaaagat atatggagtt agtagtgcaa cctgctataa
    21181 atataaatag gagtaacttt taattgtcaa aaatatataa aataaataca taaataagta
    21241 attaatagaa gatagatata aatatatttt tgaaatgctc cctgcattca taacgagtgt
    21301 agagtggcag atatctgccc aaaactcctt taattgctgg aagttcctaa agctaactaa
    21361 accacaacat aatcatgaaa taaggataag tgtgatagag tgtgcattta ataagcactt
    21421 gaggttacga aagtagaaaa aattagttag atagcataag gttaaatcct aagtgccttg
    21481 aaaatggata atcagcaggt aagcttcgaa aagaagaaac ttcaacgact agagagaaat
    21541 ctcgtaccta caagtgtggg gaaatgggga gtacctaatt tatggttagg taatgatata
    21601 gtctgtgctt aatagaaata ttaagaagtt cataggagaa ctggttagga gtaacgaccc
    21661 taattgaaca cactaaagat gtattattga ataagattca tataatattt aataatttta
    21721 attatattaa gtgaatacaa tatatgtttt tagtatcagg agaattttga aaacaaaaat
    21781 taaaaaatca agtataatca gtttagtaat tgcttttagt atggtattta ctgcatttac
    21841 accaatagta tcttatgctg acgaagtgac tagcaatgat acaatactta atggagaaga
    21901 acaatcaaat ggtcagacat cagatgtaga gaaaccatca gacggtcagg taccagatgg
    21961 ggagaaacca tcagacagtc aagtaccaga tgtagagaaa ccatcagacg gtcaggtacc
    22021 agatggggag aaaccatcag acagtcaagt accagatggg gagaaaccat cagacggtca
    22081 ggtaccagat ggggagaaac catcagatgg tcagatacca gatggggaga aaccatcaga
    22141 tggtcagata ccagatggag agaaaccatc agatggtcag ataccagatg gggagaaacc
    22201 atcagatggt cagatgccag atggagagaa gacatcagat agtcagacgc cagatgtaga
    22261 gaaaccatca gatggtcaga tgccagatgg ggagaaacca ttagatgagc aaactccaga
    22321 agaagaaaaa ccattggaag aagaaatagt tatagaggaa atgagtttaa aacaagatat
    22381 tgataaaatt cttgatatga ctttatctca aataaataaa atagtatata acttttggga
    22441 agatgaagaa gatgtaaagg cagatgaaca atcagaaatt aaccaagttt ttacttcaga
    22501 agactcattt attagcttat ggtatgacaa aaaagcaaaa gtaaaaaatt cttgtttact
    22561 aaaagaaata gatggttcag atagacctta tcatgattta agatttgatg atattacaaa
    22621 gactttaact tttgactatg taataaaggg tcttgtagga gcaagctcaa aagacgacaa
    22681 atatgtaata gaaggtgaag atggagagca aactgcattt tgttacaata atcatttaag
    22741 accaccaagt agtaatggaa agagtccata tttaccagca gaagatttta atggtcaaga
    22801 aaaccaaaat gaagaagcag ttaaatctat actatatgca ggttctgaat ttgatggatt
    22861 tggatataag caacaattta atttaggtgg agaagaaaat gaattgatga cttactcagc
    22921 tactcaaagt gcaatttgga ttattcttgg acaaatggat gaacaagaaa gacttaagca
    22981 gtatcaaggt tctataaacc tttgtgataa acttatagaa agagcaaaaa ctgaagagga
    23041 aagagctgag tatcagaaga agaaagaagt agcgataaat ataaaagctt atttagaagc
    23101 attgttaaaa gcaggtcgtg aagagttaaa gccaaataat acaggaaaac ctagtttgag
    23161 taatggttta actaagatta actttgaaaa aaatgaagat ggtacttatg aaacagaagc
    23221 tgtagcctta gtaggttata gtggtgtagt aaagttaaaa ctaccagatg gagtaactgc
    23281 ttatgatgga gatggtaata taataggtac aggcgaagta gaaatttcaa cgcaacagaa
    23341 atttaaactt aagagtgttg gaaaaccaga tgccaaagct aatatttctg ctgtatctta
    23401 tgattatatt tttccaaaag ctattcaata ttataaggca gtgcttgatt taggacaaaa
    23461 agaccattca tcttcattac catctagcaa gcaaaattta ttaagttata caatagagaa
    23521 aaagaatggt caagaagtta actttaatat aggtttacca acagatgatg atgatgttgt
    23581 aaatccacca gtgccaccaa tagatgatga tgttgtaaat ccaccggtgc caccaacaga
    23641 tgatacaaat ggacataagc caaaaccatc accaccaata gatgatacag taataaatcc
    23701 accagtacca ccaatggatg atacaataat aaatccacca gtaccaccaa cggaggatgc
    23761 agtgttaaat ccaccagtgc caccaatgga ggatgcagtg ctaaatccac cagtgccacc
    23821 aacagaggat acagtgttaa atccaccagt accatcagta agtgatacag tggaaaaaac
    23881 tccagaacta tcaagagatg atacaataat aaaatcacca aaaacaggtg atgaaactca
    23941 gctaatgtca tatgtattta ttagtgtcat tgcaatttgt ggattagcgt atcaatgtaa
    24001 aataaaaaga aattaatcta gattattatt aagtttaaaa taaaaagtct caaattgatt
    24061 tataagtcat tttggggctt ttttatgtaa atataatatg caaatatagt ttgtttaaaa
    24121 atatctattt taattatatt cttaaattta ttattggata caggcattca ttaattgttt
    24181 gatgaataga aaaatgtttt gaaggtgaaa atagtattga aaaaataata tactaagggt
    24241 ataattcatt tgatttcatt tgtataaatt taattataaa ttttaaagtg attatataaa
    24301 tttaaatgct aattacttaa taaattttaa taataattat ttaataaata attattataa
    24361 tatgctagaa taaatatata taaataagtt ttgatgaggt actactataa aggggggact
    24421 ttatgttttc gataccagaa gtaaaaaaag tagaagaggt aatggataca aaatttacaa
    24481 ctatagacga agacacaaga attgaagatg caataaaaga aatgataaaa agcaacacaa
    24541 aaactctaat ggtaatagat tcatctgacc aattaaaagg cattatatcc atgacggata
    24601 tacataatct ttatgagatg cataaaaagt atgaaggtca accagtaaaa cttataatga
    24661 agaaggatgt aatatatgtc aacgaaggtt taaccttaga tgaatgtaga gatataatga
    24721 tattaaaaaa tatagggatt cttcctgttc ttagagataa taaaattatt ggagttctaa
    24781 aacaagagca cattagggac tatctatata tgcatttaga agattatgga ttaactttga
    24841 agtatattat aggtcaaatt aaagaaggaa tttgtgctat aaataacgaa ggtgttgtta
    24901 tcttgtggaa taagtttatg gaggaaaggt atgatataaa gtctgaagat atagttggtc
    24961 gaccgatgaa tgaatttttg gaaaatacta tttcagaaaa agtactaaat agtaaagttg
    25021 gaatgagtga cttgtatttt acagataaaa aagaaaatat gtatgcatta gttcatgcaa
    25081 atcctatatt ttataaagaa gaatttatcg gtgttgtatg tacagaagtt gatgttacag
    25141 aagctaagat attagctctt gaacttgaaa aagtaaatga tacattaaaa tatctaaaga
    25201 acgaagtcaa aaatttatct aaaggtagtt ttgataagat tttaggtaaa agttataaat
    25261 tggaaaaatc gaaagcgatt gcaaaacaag tggctagaac aaatagtagt atatttattt
    25321 ggggcgaaag tggtacaggt aaagaagtat ttgcaagagc tattcatgat tatagtgaga
    25381 gaaaagggca atttatacct gttaactgta gtgccatacc aaatgagtta tttgaaagtg
    25441 agttttttgg gtatgaatca ggtgcattta caggagcaag taaaaagggt cgaataggta
    25501 tatttgaact tgcaaaagat ggaactgtat ttttagatga aattgccgat ttacctctta
    25561 gtatgcaagc aaagttgctt agagtattac aggaaaaaga aataagaaga gttggtggag
    25621 ataccacaat aaaaataaat cctagaataa tatcagctac caataaagat ttgagtaaaa
    25681 tggtaaaggc agaaaaattt agagaagatt tatactatag attaaatgtt gttgaaataa
    25741 agattcctcc acttagagag agaaaagaag acataggatt attggttcat agctttttag
    25801 aggagatatg taaacaaaat aataagcctg ttcttacaat tagcaaagat gtaatagata
    25861 tatttcagaa ttatagatgg aaaggtaata ttagagaatt aaaaaatacg attgaaaata
    25921 ttgttgtatt gtcacaaaat tctaaaatag atgtggatga tgtaccaagt tatatgatgg
    25981 attctacaaa taatagtact gaagaagaag agtatccttt agatttaacg aaggcaactc
    26041 aaaaaattga aataaagaat ataaccaagg cacttaaaat gtctaatgga aataaagcta
    26101 aagcagctaa gatattaaat atacccagaa caactttgta ttataagata gaccaataca
    26161 aaatagatgt gtctaaaata tgacacatga ctaaattttg acaaaatgat aaactttaat
    26221 ttaaaaccta gtaaagccaa cacttgtata agtgttgtgt atatagatgt gttaaaaaaa
    26281 atgaaaaagt gttgattaat tgacactttt tttgtttgag aaaatgttta caaatacaaa
    26341 aaataacata ttttaaataa atatttatat aatatttggc taaaaatagc gatagatgaa
    26401 gaatctatta tagaaaaaat ctttttgtta ataagttggc ataggaattg cttataatat
    26461 aaagtgactt taagtgagaa aaaaataata ttaatcataa caaattatgt aggttttaat
    26521 ttacttaatt tctcagttaa ggggttacct gttgaggaat tgagatttaa aataataaat
    26581 aagaaaataa tgtaaggagg tattttatac tatgtcaata actttagaaa cagctcaagc
    26641 ccatgcaaat gacccagcag tttgttgttg tagatttgaa gcgggaacaa ttatagcgcc
    26701 agaaaactta gaagatccag caatatttgc agacttagag gattctggat tattaacaat
    26761 accagaaaat ggattaacta taggtcaagt actaggagct aagttaaaag aaactttaga
    26821 tgcactttct ccaatgacta cagataacgt agaaggatac aaagcaggag aggctaaaga
    26881 agaagtagta gaagaaacag tagaagaagc agctccagta tcagaagcag cagtagttcc
    26941 agtaagcaca ggagttgcag gtgaaacagt taaaatacac ataggtgaag gtaagaacat
    27001 aagcttagag atacctttat cagtagctgg tcaagcagga gttgctgctc cagtagcaaa
    27061 cgttgctgct ccagtggcaa gtgcagcagc agaagtagct ccaaaagttg aagaaaagaa
    27121 acttttaaga agcttaacta aaaaacactt taaaatagat aaagttgaat ttgctgatga
    27181 aactaaaata gaaggaacta ctttatacat cagaaacgca gaagaaatat gtaaagaagc
    27241 taatgaaact caagagttag ttgtagatat gaagttagaa ataataactc ctgataaata
    27301 tgaaacttac agtgaagctg tattagatat acaaccaatc gctactaaag aagaaggcga
    27361 attaggttca ggtataacta gagttataga tggagctgta atggtattaa ctggtacaga
    27421 tgaagatgga gttcaaatag gtgaatttgg ttcttcagaa ggtgagttaa atactactat
    27481 aatgtggggt agaccaggtg ctgctgacaa aggtgaaata ttcatcaaag gtcaagtaac
    27541 aataaaagca ggaactaaca tggaaagacc aggaccttta gctgctcacc gtgcatttga
    27601 ctatgtaact caagaaataa gagaagcatt aaagaaagtt gacaactctt tagtagttga
    27661 tgaagaagta atagagcaat acagaagaga aggtaaaaag aaagttgttg ttataaaaga
    27721 aataatggga caaggtgcaa tgcatgataa cctaatatta ccagttgagc cagttggtac
    27781 attaggagct caaccaaacg ttgacttagg aaacatgcca gttgtattat ctccacttga
    27841 agtattagat ggtggtatcc atgcattaac ttgtatagga cctgcatcaa aagaaatgtc
    27901 aagacattac tggagagagc cattagtaat aagagctatg gaagacgaag aaatagattt
    27961 agtaggtgtt gtatttgttg gttctccaca agtaaatgct gagaaattct atgtatctaa
    28021 gagattaggt atgttagttg aagctatgga agttgatgga gctgtagtaa ctactgaagg
    28081 tttcggaaac aaccatatag atttcgcatc tcacatagag caaataggta tgagaggtat
    28141 accagtagtt ggtgtaagtt tctcagctgt tcaaggtgct ctagttgttg gtaataaata
    28201 catgactcac atggtagaca acaataagtc taagcaaggt atagagaatg aaatattatc
    28261 taacaacact ttagctccag aagatgctgt tagaataatg gctatgctta aaaatgctat
    28321 agaaggtgta gaagttaaag ctcctgaaag aaaatggaat ccaaatgtta aattaaataa
    28381 catagaagct atagaaaaag ttacaggaga aaaaatagta ttagaagaga atgagcaatc
    28441 tctaccaatg agtaagaaga gaagagaaat atacgaaaaa gacgaaaact aaaatttaga
    28501 tactaactaa gatttagaca ataacataaa tttagatcca agtttaaaac agtatagaga
    28561 ggtagaatta taatggatag gttaaaatat atctcatcag agacttttta tgaaggtatt
    28621 atagttgaca tcaaaggcgg tggagtgact atagacctaa aaggtagact tggacaattt
    28681 aaaataccta atagaatgct tataactgac tacgaactta agataggtca agaagttgga
    28741 ttcatgctaa gctatcctga ggtattaagt ccagagccta atcaagagta tgtagaaaat
    28801 attaggagag aaaaagaaaa acaagcagaa atgcaatcaa aacaagaaga aaaataataa
    28861 ataatcgttt gaaaatagaa gggagaggaa atatatgagc cttacaacag tacaaggact
    28921 tcaatctgaa atattcgttc caataacacc tcctccagtt tggactcctg taactaaaga
    28981 attaaaagat atgactatag ctttagctac agcagctggt gttcatttaa aatctgacaa
    29041 gagatttaat ctagcaggag atactacatt tagagctata cctaatacag ctacagttga
    29101 tgaaatgatg gtatcacatg gtggatatga caatggagat gtaaacaaag atataaactg
    29161 tatgttccct atagatagat tacatgaatt agctgcagaa ggatttatca aaggagtagc
    29221 tcctatgcac tatgcattca tgggtggtgg aggaaaccaa catgtcttca ctgaagaaac
    29281 tggtcctgct atcgctgcta aacttaagga agagggagta gacggtgtag ttatgacagc
    29341 tggctgaggt acttgccata gaactgccgt gatcgtgcag agagcaatag aggaagctgg
    29401 aatacctaca ataataatag cagctcttcc tccagtagtt agacaaaacg gaactcctag
    29461 agcagttgct ccattagttc caatgggtgc taatgctggt gaaccacata atatagaaat
    29521 gcaaacacat atattaagag atacattaga gcaattagtt gcaataccat ctgctggtaa
    29581 gatagttcca ttaccatacg aatataaagc tcacgtttaa aatgtaattt atatttgaaa
    29641 atatagaacc cttctcctac acggttagga gaggggtttt aagaacaaag gtggatatta
    29701 tggaagaaaa aataatgaga agacttgtta taaaaccgtt tcatatgaat gaagttaatt
    29761 tcggttcaaa aacttctata aagaaagatg tacttacaat agatttatca agtatagatg
    29821 aaataaaaga aagagaagag ttaataacag acataaaagt agacattata aaacctggag
    29881 attatgacag agaaattaat actattatgg acattatacc aatatcaaca aaagttttag
    29941 gcagactggg agagggtatt acacatactt taacgggtgt atatgtaatg ttaactggtg
    30001 cagatgaaga tggaaatcaa atgcatgaat ttggttcttc agaaggaatt ttgaaagacc
    30061 aaatggtatt tgggagatat ggaactccat cagttgaaga ttatataatc catgtagatg
    30121 taactttaaa gggaggttta ccttttgaaa gaacacttcc acttgcagca ttcagagcat
    30181 gtgatgattt tatacaagaa ataagagcgt ctttgaaaat ggaagacgga agaaatgcta
    30241 ctcaagttcg tgaatatttt gacaaaattc gaccaaatgc aaaaaaggtt gtcatagtaa
    30301 aacaaatagc aggtcaaggt gctatgtatg acaatcagtt attctcaaag gaacctagtg
    30361 gatttgaagg aggaacatca attatagata tgggaaatgt acctatgatt atatctccta
    30421 atgaatacag agatggtgcc cttagagcta tgacttaaga gaggtggata ttatgggaat
    30481 aggaccttca acaaaagaaa catctcttca tcactttaga gacccacttt tagacatagt
    30541 tgaaagtgat aaggatgtag atttacttgg agtaatagta gtaggaactc cagatggaaa
    30601 tgaaaataaa acctttgtgg gacaaagaac ggccgcttgg ctggaagcca tgagagtaga
    30661 tggagctata gtttcatcag atggttgggg aaactcacat gttgactacg ctaacacatt
    30721 tgaggaaata ggaaaaagag atatacctgt agttggagtt acatttaatg ggactcaagc
    30781 taagtttgtt gtaagcaatc aatatatgga taccatagtt gatatgaaca aatcaaaaga
    30841 aggtattgag acagaagtag ttggagaaaa caacacaaat gaaatagatg ctaaaaaagc
    30901 tttggcattt ttaaaactta aaatgagaaa acatggataa aaaatttaag taagggaatg
    30961 agttttgtgg gaatttctac atcaatgaaa aacacatcac ttcatatatt tagagacccg
    31021 ttactagatt tgatagataa tgattctgac atagacttgt taggagtggt tgtagttggg
    31081 acatcagagc acaatgaatg gaaaacattt ttagcaacta gacttggaag atggattgaa
    31141 tcattgcgac ctgatggagt gattataact ttagatatgg caggaaacca gcacattgat
    31201 tttacaaatg ctatagcaga gtttgtaaaa tcagatatac ctacagtagg tcttactatt
    31261 atgggagcag atggattagt aattaccaat ccatagttgg ataaagctac cataattgat
    31321 tataaaaaga caacaggcta catagaaaca gaagtagttg gagacaatca catggatgaa
    31381 gttgatgtta aaaaagcatt ggctttccta aaattaaaaa tgcgtaaaga cgcaaaataa
    31441 acctaagggg gaatttgata tgaaatttag cagaagtata caagctatag actctcatac
    31501 agcaggtgaa gcaactagaa tagttgtagg tggaatacct aacataaaag gaaattctat
    31561 gcctgagaaa aaagaatatt tagaggaaaa tctagattat ttaagaactg ctataatgtt
    31621 agagccaaga ggacataatg atatgtttgg ttctgtaatg actcaacctt gttgcccaga
    31681 tgctgacttt ggaataatct tcatggatgg tggcggatac cttaacatgt gtggccatgg
    31741 tacaatagga gctatgacag cagctataga aacaggtgta gttccagcag tagagcctgt
    31801 aactcatgta gttatggaag ctccagcagg aataataaga ggagacgtta cagttgtaga
    31861 tggaaaagct aaagaagtat cattcttaaa cgtaccagct ttcttatata aagaaggtgt
    31921 tgaagttgac ttaccaggtg ttggaactgt taaatttgac atatcatttg gtggaagctt
    31981 ctttgctata atacatgcaa gtcaattagg tttaaaaatc gaacctcaaa atgctggtaa
    32041 attaactgaa ctagctatga aacttagaga tataataaat gagaaaatag aaatacaaca
    32101 tccaacttta gctcatataa aaactgtaga cttagttgaa atatatgatg agccaactca
    32161 tccagaagct acttacaaaa atgtagttat atttggtcaa ggtcaagttg acagatctcc
    32221 atgtggaact ggaacaagtg ctaaattagc tactttacat gctaaaggtg aattaaaagt
    32281 aggagaaaaa ttcgtatatg aaagtatatt aggaacttta ttcaaaggtg aaatagttga
    32341 agaaactaaa gttgcagatt tcaatgctgt agtacctaaa ataactggtt ctgcttatat
    32401 aactggattt aatcattttg taatagatga agaagaccca cttaaacatg gatttattct
    32461 taaataagta ataaataaat taggaaatta aaatagaata gtagccactt gtcggttgct
    32521 attctatagc ttttaggcta taagattcag aatatatgaa aaaataaaaa aataggagga
    32581 tggattatgg tagcaatctt aaaggcatta tatgtagtat tcgcagcaat ctttgcagtt
    32641 gtgtttggta aagacgtagc taaagcaaac aaagagggga aactagaaga acaaagtact
    32701 ccaaaagttg ctgtaacagg atttataaca gacttctttg atacattagg aatagggtca
    32761 tttgctccaa caatagcaat gtcaaaggct ttaaagttaa atataccaga taaaaaaatg
    32821 cctggtacat taaacgtagc acatactatt ccagttgtta cagaagcatt catatttaca
    32881 tcaatcatac cagttgatgg tgttacttta gtatctatgg ttgtggcagc agcagttggt
    32941 tcatatatag gtgcaggaat aatagctaag atggacgaga gaaaaataca acttgttatg
    33001 ggtgtaactt tagcattaac agcaatatta atgttattag gtcatccttg gattaacgta
    33061 ttaccaggtg gaggaaatga attaggtctt actggtgtta aattagtaat aggtgtaata
    33121 ggtaacttta tactaggagc tttaatgaca gctggtatag gtttatatgc tccaggtatg
    33181 gcaatggttt atttcttagg aatgtctgca aaagttgcat tcccaatagt tatgggttct
    33241 tgtgcattat taatgccagt tgcaagtatg aaattcataa aagaagatgc atatactaaa
    33301 aaagcatcag ttataatagc tatatgtggt ttaataggag ttttcgtagc tgcatatata
    33361 gtaaaaactc taccaatgga tatattaaaa gcattagtaa tagtagttat agcttatact
    33421 tctgctacaa tgataatggc tgcaaataaa aacaaaaaga ctgcttagtt aaagactact
    33481 tagtgtttat taaatattac agctaataat aaagattata aactgcttta aaatattgaa
    33541 ttaatatttt agggcagttt tttatttact aaattaatat aaagcaatta acatgccaat
    33601 tatgtaatat atatagtgat ttagtagatt taatgtaaaa aacgttgtat ttttaaagaa
    33661 taaagcataa tttaaagcta aatatttaaa ttttataatc aataagttca ataaatgttc
    33721 atatattaat acagattaat aaaaatataa cctataataa gtctaatata acttataatt
    33781 aagaaaaata agtttataat gactcaatat tcatctgaat tatttgaata aatttaattc
    33841 gttgaagttg cttatataga gtgaataaat ttaagttata
//
LOCUS       DBGJFU010000031        33747 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.031, whole genome shotgun sequence.
ACCESSION   DBGJFU010000031 DBGJFU010000000
VERSION     DBGJFU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.031"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(16..1383)
                     /locus_tag="ACOIAS_001438"
     CDS             complement(16..1383)
                     /locus_tag="ACOIAS_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2701154.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(1633..2070)
                     /locus_tag="ACOIAS_001439"
     CDS             complement(1633..2070)
                     /locus_tag="ACOIAS_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HGZ2701155.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(2102..2731)
                     /locus_tag="ACOIAS_001440"
     CDS             complement(2102..2731)
                     /locus_tag="ACOIAS_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HGZ2701156.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(2765..3652)
                     /locus_tag="ACOIAS_001441"
     CDS             complement(2765..3652)
                     /locus_tag="ACOIAS_001441"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HGZ2701157.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(3681..4901)
                     /locus_tag="ACOIAS_001442"
     CDS             complement(3681..4901)
                     /locus_tag="ACOIAS_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6080 domain-containing protein"
                     /protein_id="HGZ2701158.1"
                     /translation="MSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDLNNTYDV
                     LLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTDFNSAYG
                     FGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNVERFIYS
                     QFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKIGKMLKH
                     LITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLLYSVFYF
                     PGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIIIFNLTLH
                     GIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIVISNVNG
                     FIEILNIGIKSYPV"
     gene            complement(5303..6445)
                     /locus_tag="ACOIAS_001443"
     CDS             complement(5303..6445)
                     /locus_tag="ACOIAS_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGZ2701159.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(6586..7452)
                     /locus_tag="ACOIAS_001444"
     CDS             complement(6586..7452)
                     /locus_tag="ACOIAS_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HGZ2701160.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(7786..9333)
                     /locus_tag="ACOIAS_001445"
     CDS             complement(7786..9333)
                     /locus_tag="ACOIAS_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2701161.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(9326..10462)
                     /locus_tag="ACOIAS_001446"
     CDS             complement(9326..10462)
                     /locus_tag="ACOIAS_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701162.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(10725..11822)
                     /gene="dinB"
                     /locus_tag="ACOIAS_001447"
     CDS             complement(10725..11822)
                     /gene="dinB"
                     /locus_tag="ACOIAS_001447"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGZ2701163.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(12212..13717)
                     /locus_tag="ACOIAS_001448"
     CDS             complement(12212..13717)
                     /locus_tag="ACOIAS_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HGZ2701164.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(13979..14329)
                     /locus_tag="ACOIAS_001449"
     CDS             complement(13979..14329)
                     /locus_tag="ACOIAS_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HGZ2701165.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(14552..14743)
                     /locus_tag="ACOIAS_001450"
     CDS             complement(14552..14743)
                     /locus_tag="ACOIAS_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701166.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(14861..15775)
                     /locus_tag="ACOIAS_001451"
     CDS             complement(14861..15775)
                     /locus_tag="ACOIAS_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2701167.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            complement(15909..17405)
                     /locus_tag="ACOIAS_001452"
     CDS             complement(15909..17405)
                     /locus_tag="ACOIAS_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2701168.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            complement(17685..19175)
                     /locus_tag="ACOIAS_001453"
     CDS             complement(17685..19175)
                     /locus_tag="ACOIAS_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2701169.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            19642..19842
                     /locus_tag="ACOIAS_001454"
     CDS             19642..19842
                     /locus_tag="ACOIAS_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGZ2701170.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            complement(20008..20886)
                     /gene="speB"
                     /locus_tag="ACOIAS_001455"
     CDS             complement(20008..20886)
                     /gene="speB"
                     /locus_tag="ACOIAS_001455"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGZ2701171.1"
                     /translation="MKNNFYHMNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(20876..21727)
                     /gene="speE"
                     /locus_tag="ACOIAS_001456"
     CDS             complement(20876..21727)
                     /gene="speE"
                     /locus_tag="ACOIAS_001456"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGZ2701172.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            complement(21757..22170)
                     /gene="speD"
                     /locus_tag="ACOIAS_001457"
     CDS             complement(21757..22170)
                     /gene="speD"
                     /locus_tag="ACOIAS_001457"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGZ2701173.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            complement(22257..23732)
                     /locus_tag="ACOIAS_001458"
     CDS             complement(22257..23732)
                     /locus_tag="ACOIAS_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HGZ2701174.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            complement(24370..25023)
                     /locus_tag="ACOIAS_001459"
     CDS             complement(24370..25023)
                     /locus_tag="ACOIAS_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2701175.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPIAEMKKEKLL"
     gene            complement(25268..27133)
                     /locus_tag="ACOIAS_001460"
     CDS             complement(25268..27133)
                     /locus_tag="ACOIAS_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HGZ2701176.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(27138..29558)
                     /locus_tag="ACOIAS_001461"
     CDS             complement(27138..29558)
                     /locus_tag="ACOIAS_001461"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HGZ2701177.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     TEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(29572..31014)
                     /gene="glgA"
                     /locus_tag="ACOIAS_001462"
     CDS             complement(29572..31014)
                     /gene="glgA"
                     /locus_tag="ACOIAS_001462"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGZ2701178.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(31043..32173)
                     /gene="glgD"
                     /locus_tag="ACOIAS_001463"
     CDS             complement(31043..32173)
                     /gene="glgD"
                     /locus_tag="ACOIAS_001463"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HGZ2701179.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(32175..33335)
                     /locus_tag="ACOIAS_001464"
     CDS             complement(32175..33335)
                     /locus_tag="ACOIAS_001464"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGZ2701180.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT        11086 a         5934 c         3446 g        13281 t
ORIGIN      
        1 tttatattgt atatcttatt cttttattgc tttaggtttt gatagaaaac atttataaaa
       61 tataaagaaa gaaattgctg ttatagtagc agctgttata tctgcaactg gctctgctaa
      121 aattactgca aaaagttttt cattaaagaa tgttggaagt ataaatatta aaggtattaa
      181 aagtacaatc tttctaagta gagctaaaac caaagatatt gttgcttttc caagtgctaa
      241 aaatgtctgt tgacaagcta tttgaatccc aaacataaaa gcaccagcaa aatatatttt
      301 tatactccaa ctagtcatac taagtaactg cggattctta ttaaatatac taacaaaaaa
      361 tgcaggaaat atcattaata aaagccataa aatagtagaa tatgtaaaac atgacaacaa
      421 acacaactta aatgtctttt ttactctatc aagttgacct gaaccataat tataacttat
      481 tattggttgt gctccttgtg taacccccat attaggaagc ataagaattt gcattatact
      541 gctcattata gccattgaac ctactgctaa atctcctcca tacatttgta atttactatt
      601 taaggctata agtactaaac tttctgtaga ttgcattata aatggagaga ttcccaaacc
      661 aagcactggt aagatgatac ttgcttttgg tatcatatac ttctttttta ttttaagtat
      721 acttttcttt ccaaagagaa acataagtac ccaaatagca gaaacgcctt gtgcaattat
      781 agtagctaat gcagctcctt ttacacccat attaaagcca aatataaaaa tcgggtcaag
      841 tattatgttt attaaagcac ctatagcaac tgtcatcatt ccagttgtag caaacccttg
      901 ggtatttata aaagtattca tacccattga aatctgaaca aacactgtac ctattaaata
      961 aatatttaaa tagtccattg aatatccaat tgtagattca cttgctccaa acatccaaag
     1021 taaaggctct tttccaatct taaaacctat agtcaaaagt attgctaaaa tcaccagtac
     1081 agaaaaactg ttactcatta ttttctctgc ctcatcattg ttttgctcac ccatttttat
     1141 agcagctaat ggacctcctc ccataccgat taaataacta catgcagtta ttataattat
     1201 tattggaaat gccactccaa caccagccat agcaacttct ccattttcca ttctaccaat
     1261 aaaaatccta tcgactatgt tgtataaaac atttacaatt tgtgcgataa tagcaggtgt
     1321 agcaagttta aataataatt tacccacact ttcactacct aagttagtgt ttgaattttc
     1381 cattttaaaa cctcctcaat ccaataatta aaatgtatca ctaaaatttt attctatata
     1441 aaaatttcat tttattagca gacaaattta gagtactttc cctacaagta atactatact
     1501 atatttcttt tgccatagta aacattttag aactaactga agttaaatac tttatctcat
     1561 tttttattta ttattaaaaa tttttcacac atactcataa aaaaactatc caagtatatt
     1621 acttagatag ttttatatta tatgtaaaga aagagctgaa actataaccc ctgataacaa
     1681 attgccaaga aatactccca taaaagcatc ttttattctc atttttaata tggaagctat
     1741 agcagatgca gtccacgttc ctgtagttgg cagtggtata cctacaaata gtattatacc
     1801 aattatcgaa acacttttta atttcttcat tcttgagttt atttttctat caattacatc
     1861 agcaatccct ttaaatagtt tcttccctct taaaaattgt aatacatgtc tgaatgtcaa
     1921 tatcaaggga atcgatacca atgtagaacc tattacacta aatacataaa cccatatagg
     1981 atttaagccc attgcaatac ctataggaat ggctcccctc aattctgtta caggaaccat
     2041 agataaaatt aatatgtgta aatatcccaa actattccct cctattctaa gtgacattaa
     2101 gttatgaatt tatttgctct ataacctcac catccttttt actaactaaa aatgcatttt
     2161 tagtcaatga aagcattggt aaatcactct tagagtctga atatgcatat gaattttcat
     2221 aatcaatctc tatcccaagt ttacctaata cagcctttat tctttttact ttttcaattc
     2281 ccttacagtt ttctccaatg aatttatttt tatatcttga attttcataa aaaaactccg
     2341 tgcctataac ctcgtctgca agaccataat acttaaaata tttcatatat gcatatggag
     2401 atgcagttat cataattata acattatctt cttctttatt tttgtaaata acctttttca
     2461 tagattcgtt aatttttttc attatgcaat catcaaaaaa cttttttaaa tcttcttcac
     2521 taaaatctac tattggataa aagattgcct cttttgcctt cttcaatggt attattctaa
     2581 ttaaatacat aaatagaata aaaataatgt atggtaactt tataatagtt ttaaaggttt
     2641 tattttttaa agagtaaata aaaaaatcta tcatgctatc agtatttata acagtataat
     2701 ctacatcaag taataataca ctttttttca taagtatttc cccatcctat cactaaaaat
     2761 atcactaaaa aatcaatatt attaaacatg taattatcca taagattacg tttattttta
     2821 aagcattatc ttttaagaca atatcagtag gattattttc atttgtagtg ttaattatat
     2881 aattatatct aagtacacca taaactacaa atattgttgt aaatatcata tgtggctttt
     2941 ctggattact tgtagaatac aatgcataac atacaattgt agaacttaat ataactatca
     3001 tcatttgatt tagattttct atgtcataat cttccaatat ttttctatgt tcaactgcat
     3061 catctcttag agtttctatt tcttttttac gttttccaag agctaagtac agagataaga
     3121 aaaatgtaca taaaattatc caatttgata aactaacact tatggctaca catcctgcat
     3181 aaaccctaag tataaaacca agagatattg aaaacacatc taatagtatt atattcttta
     3241 gtttaagaga atataatata ttgtttatca aatagagact gagaattata gaaagattaa
     3301 aactaacaac tgttgataaa cagactgaaa ttaaagctaa tactacaaat agtatattag
     3361 cacttttttt gcttattctt ccacttgcca ctggtctatt acatttttta ggatgttttt
     3421 tatctttatc catgtctata gcatcattca gtgcatatac tgatgatgac attagacaaa
     3481 aacacacaaa cgcaataaac gtatttaaaa ataaatttat atttagtatg ttgtttgaaa
     3541 aaattatagc tgaaaataca aagaaattct ttatatattg ctttattctc attaacttta
     3601 tataatctga aattttagta agaagcctta tacttgtatt ggtctgtttc ataaacttac
     3661 taccctttca atattatttt ttaaacagga tatgatttta tccctatgtt taaaatttct
     3721 ataaatccat tgacattact gattactata gtaaccaata acagcgatag aaatatatta
     3781 tacatattct tttcattttt tctaagcgct ttcgtaaagt aagccaataa aattataact
     3841 gcaaaactaa aatttatagc cataatactt gaattgacta gattatatcc tactatacca
     3901 tgtaaagtta gattgaatat aattattccc atacatagct taggaactct tttttctgaa
     3961 ttttcaataa ctgataaaac acaaaaacat aaagctaata atgtaagaat tactggtatt
     4021 gtagcaactt ctccattttg caaaaataac ccttttttaa tacttgctcc aggaaaataa
     4081 aaaaccgaat acaataatct ctctataatc attgcaatct tttccaaaat tggttctcca
     4141 cttataaatt tcgtatcata ctgtattaca tttccaaggt tcataaaact ctcataagac
     4201 tttgttgaaa ctacacaaat atatgccatt aggataaatg taattaaatg ttttaacatt
     4261 tttcctattt ttaatttaaa ctcaaatatc atattaaaaa aatacaaata tatattacta
     4321 atagttattc caaatagagg aatagctgct aattctacta aataagaatt ttttcctttc
     4381 atcttatttg caataactat gaaaaatatt aatgaaaatt ggctatatat aaatctctct
     4441 acatttaaag tcataaataa ctgtggaaaa gaaaatatca ttatcatagt aagtaaaatt
     4501 aaagttaatc tctgcatttt tatcttttta atatgattaa agactaaagt tattgacata
     4561 gcactagtta ttatttgtag acaaattagt ccaaacccat aggcactatt aaaatctgtt
     4621 gtcccaggat ttgctattga agtacaaaat aggtatattg gatatgcaaa aattgatata
     4681 attgctgaaa acaatatgtg cttactattg tcttgactta tagcaaatac atttaaattg
     4741 aataaaactc ctgtatctgt atctaacaat acatcataag tattgtttaa atcataaaat
     4801 atagtagtct tagaaaaaaa tatcttagtt attattaagt aagttattac aactaatata
     4861 aacaaaaaca tctcgttttt atattttaat attttactca tcaatatatc accatccatt
     4921 cattatatag gatttgctga cacctacaat ttattcctta tatttaatag ccatcttcat
     4981 aatgttctta cttgttttat tgtaattgaa taactcttta tttacaacca caatatttac
     5041 tttattgatg ttataatcag tcaagtattg tttatataac atagattagt gttgtataaa
     5101 atgagaattt gtaagcaata ttaatgtaaa atatttgaaa aactttatat aaaaatatac
     5161 aatgttgctt atggaattaa actataacaa attaaagcat aatttaagga gacaaaatta
     5221 taatctgtct ccttaaatat taaaactata ttttaaaaca ttactaaata ctatacaaat
     5281 ttcaaatata gtataaaatt aattagactt taacactatt agctccatct tcttcccatt
     5341 ttttaatttc ttttaatcta tctattatag gtaaatgctg agtacattca acttcacatt
     5401 ttccacaaga tatacattct ttagcttttg ccttactatt cattgtgtaa ttccaatgtc
     5461 ctaagccata aactaacttt gtcatttctt catcactctt tttaaacatc tgcttttcat
     5521 tataaaaaag catataggct ggggtattaa taccttttgg acatacttta caataaccac
     5581 aacctgtaca tatttcgttc atattcttat ttaatctagt ctcaatatca tgtatatcct
     5641 catctgaata aattctattt tcgtctgcaa ttttacatgc atcatctata tcttgttttt
     5701 tattaaaccc tatcaaagat atacttattt gtgaaattcc tatgttgaat cttaaagctg
     5761 attctatagt actttcgcca tcttttgata gaaaagaaag ttctttatta tactttggaa
     5821 tagttccacc acttaatggg ttcattgcca caacaccata tccattatca acagcatatt
     5881 tggcaccttt tagcctatat ggaaagttta atatatttat tcccatagtt acaccttcaa
     5941 acttattttc acttagaata tctataacct catctcctgg ttgatgagat gaaaatacta
     6001 tatggtctat caatccttct tctttacatt tcaataatcc ttcatattga cctccaggtc
     6061 tcattgctag ttcgtaatgt tccatctttc taatattcca aacatgataa aaatttattt
     6121 tagatacgcc taatcgttct attgattttt ttacagcctc tatagctttt tctgatgtat
     6181 cacatgcttt tggcttttct ttagttgata cataaaaatc ggtcttacct tcctttatca
     6241 tttgtctaaa ggctagtcca aatatatctt cacttctgtc atcacaatac cctggtgctg
     6301 tatcaaagta attaatacct ttttcatacg catatttgac taaatcagca ttttcttgat
     6361 tacttttttc taaatcaaac cttaatcctc catagccaac tactgaaacc attttccctg
     6421 tctttccata ttcaacgtat ctcattgtaa attcctcctt agatatatta agacttaatt
     6481 tctttataga aaatattata ccataaataa tttttataaa ttatttatgg tataatattt
     6541 tttttataaa ttatattttt ttataacttg tattttataa ctaagttaaa aactataaaa
     6601 aggttcaata tggactatta cttgagcatt ttcattaatt tcttcttgta tttgtttctc
     6661 aattttatgt gttaaatcat gagattgctc tacgcttata aatgggtcta ccattacatg
     6721 catgtcaata taaatagaac ttttgctacc tctacttctg atattgtgaa catcccttac
     6781 ctcattaaat tcaaacacaa tttcttttat ataatcctca tctacagcca ctctatcgac
     6841 caaaattcca atagatggcc taaatattcc ataagccgaa taaattatga atgcagaaat
     6901 aataatagga actatagatt ctattattac tggaaatcct aacttaacac caactaaagt
     6961 aaccaataca cctagagaca caaatatatc acttcttgta tgcaatgaat ccgaaatcaa
     7021 aacataacta tttagtttag tcccaattct atattcatac atgcatacaa aaatatttat
     7081 aaaaagtgta ataattaaag caattaaact ttctatagtt atagcaggta caactggatt
     7141 agctattctt aacactgcac taagaattat cttaattgct ataacaaaca acataccact
     7201 tataaataaa ctggttataa tttcaaactt tttatgtcca taaggatgat ttttatcttt
     7261 tggtttagat gcaaagaaaa tacctataag ccccactata ttagatgctc catctgacaa
     7321 agaatgaaat ccgtctgctg tcatactata gctttttatt tgattaccaa taatgatttt
     7381 taataatgct actgcaaaat tagcaaataa aattatccac aatacttgct tcactttctt
     7441 atagttatcc ataacaaatt ccccctccct tcgattcaat aaagaaagct atctatcaat
     7501 atcaatcaac cagacaataa aatcgtatat aagcacattt actatattta tattcaaaat
     7561 tggttgtact tgttcattct atttgaaact taattataaa taaaaatcgt aggtatggtt
     7621 ttggtataca taaaaataag agactgtctc aaaataaaga taatcttctt ttttattgtt
     7681 gaaatgataa aatatatttg atttcatata aaaaagagtg cctcatgaac taaaaagttc
     7741 attttgagac actcccttaa atttttgata taaactatta attttttata ctaagccttc
     7801 tttaactaag aatgaatgag caacttcttc tggtgattta ccaagttcat ccacttgata
     7861 gttaagttct ctcatagttt cttcatttat ttttcctgaa agcatattca taactttttc
     7921 taactcagga tatttcttca aagtatcttc tcttactaat ggtgctgcat aatatggtac
     7981 aaagaaattt ttatcgtcct ctaatatctt aagattaaac tttttaataa gaccatcagt
     8041 taaaaatgca tctataacct gagattcatt attatctaaa gctgtatatc ttaaacttcc
     8101 attcattgct tttacatctt tgaattttaa accataatct ctagaaagcc ctacaagacc
     8161 atcttctcta ttttcaaact ccaaagttgg tgagaatgta aattctttac catatttagt
     8221 caaatcagaa atagtattta ttccatactt ttcagcagtt tcaggcatca tagctaatgt
     8281 gtaagtatta ttaaatccaa gagggtcaag taaagttaac tgattttctt tttccatagt
     8341 atcttttaca acattatatg cttcatcagc atctttaatc ggagcatgtt tcattacatt
     8401 taccaaaagt gtacctgtat agtcaacata catgtctaac tcaccagatt taattgcatt
     8461 aaacgcaaca gatgaaccac ctagatttaa attcttctta actttaagat tcgtattttt
     8521 ttctactaat tcagcataaa cattgccaag tataagttgt tctgtataat ctttagaacc
     8581 aatagttatt gtatcttttt tacttgaaaa actagaacta aaaacaacga acaacattga
     8641 aacaacaagc actacagata ttactttaag tgctatataa ttcttcttag gtgcttttgg
     8701 atttaatcct tttggagtaa ctgcaacttc aattttacta aataaataat caactaacag
     8761 tgcaattata catgatggga ttgcccctgc taaaatcata ttattattta cagtttgaac
     8821 ccctgaaaat actaaatatc ccaaaccacc agcacctata aatgctgcaa gagtcatcaa
     8881 accaacagct gtaactgcag atattctaac tccacccatt attataggaa gcgctaaagg
     8941 aaattgtatt ttaaataatg tctgatttct tgtaagacca atacctttag ctgattctaa
     9001 tactactggg tcaatacttg ttatacctat atacgtattt tttacaattg gtagtaatga
     9061 ataaaccact accataaata tggctggttt acttcctata ccaagtatag gtacaagaag
     9121 acccaaaagt gccatagacg gaactgcttg gactaagttt acaaatccaa gtacaaactt
     9181 tctaagagat gagattcttg aaataattat tccaagagga acaccaacta ttatagctat
     9241 taaaatagat gtaacagtta gactcatatg ttgtattaaa agctctataa tcttatcttt
     9301 ttgtaatagt ataaagttaa aaaaattagc caagaacact cacctccatt tctaagaatt
     9361 gttcacttaa aacagaaagt aaactacttc tagtaataag ccctaccagt ttattttcat
     9421 cagaaataac cggaatatat ccaaccgaat tacgattcat tacatttagt atttctacta
     9481 aattatcacc ttcatttaca tgcaaaggct cacttgacat aatatcagca agtaatattg
     9541 acttatcatc aatatctttc atatctttta cagtaacaat accttttaat gtttttgctc
     9601 tatctataat tagtaagcta tcaaccttac tcgttctcat aatttctatt ccttgagtta
     9661 cagttctagt agaattaaca gcaataggat tttttatcat aatatctctt gcttttatat
     9721 attctggatt atcccaaact ctatcttctc cgataaaatc ttttacaaag tcatttgcag
     9781 gctttctaag tatattttcg ggagtatcat attgtgctat tcttccatcc ttcataatac
     9841 aaattttatc agctatcttt aaagcctcat ccatatcatg agttacaaat attatagttt
     9901 ttttcaattc atcttgaaga gaaaataatt gttcttgtaa agatgttctc gttattgggt
     9961 ctaaagcact aaaaggctca tccatcaata ttatttcagc atcagtagca atggctcttg
    10021 caacaccgat tctttgttgc tgtccaccac taagttctga aggatactta ttcaaaaatt
    10081 catcactatc aagtcctacc atctcaagta gttgcaacgt tcttttttca atttcttcaa
    10141 cggatttttc cttttttagg cgtggaatta gctctatatt ttcttttatt gtaagatgag
    10201 ggaataggcc tgtgttctgt ataacatatc ctatttttct tctaagctct atttcatttt
    10261 cctgagaaat tggtttgcca ttaatatata tctcaccaga tgtaggtttt attaatttgt
    10321 ttataagctt caaagtagta gtctttccac aaccacttga accaatcaaa actaccaatg
    10381 tacctgtttc tacaactaga gaaatatcat ctaatataac attatttcct attttttttg
    10441 ttacatttct tatctctatc ataatttatc atcccctttt tcgaacaagc aaccttcttc
    10501 aatattataa gttgttttta taattatatc atactatttt atttatgtaa acaaagtacg
    10561 atttttaggt cactatttgt ctgaatattg ctacaaatat aaagtattta tatatagtat
    10621 ataatacata atgtcaaaaa acaaaaaata taaaaatatt cataaaataa cactagcctc
    10681 aattatatag tattaaagct agtgttattt catttaaaca aaactcacat ttggatacta
    10741 tccatgtatt caaataatgt aatatcttta taagtttctt ttttatcact taggtttgat
    10801 attgtaactc ccaatagcct aatttgtttt ccattaacat caacatttct caacaaatta
    10861 tctacaccac ttcttatatc attatattcg tcaatatagt gttctaaact aaggcttcta
    10921 gttatctttg taaagtcctc atatttaatt tttaatgtta atgtttttcc aaacttctca
    10981 gagtttttta atctatgaca tacttcttca caaagttcat caagaatgtt gcgtgtttct
    11041 tcttcatcaa tatccaaatt atgacttaat gtagtctcag ccccaacaga ttttctaacc
    11101 ctatttggtt ccactggtct atcgtctatt cctctagcaa atttatataa ttcataacct
    11161 ctttttttga atatattttc aagttcaaat aaatttaaac accttaaatc atatccagtt
    11221 tttataccta aatttttaag agtatttgaa gtaacttttc caacaccaaa aaatttattc
    11281 acaggtaatt tatctaaaaa gtcctgggca ttttcttcgg ttattacagt taacccattt
    11341 ggctttctta agtctgatgc aatcttagca agaaacttat tgtatgacac accagctgaa
    11401 gaagtaagac caacttctct aaatatatct ttttttattt gctttgctat ctcaattgat
    11461 gaatcaatat tttttttatt ttttgttaca tctaagtaag cttcatctaa agataatggt
    11521 tcaactaaat ccgtgtatct ataaaatata tctcttattt gctctgacac actcttataa
    11581 acatcaaatc gaggtcttac aaaaactgca tatggacacc tattgtaagc tacagctgat
    11641 ggcatagctg aatggatacc aaattttcta gcttcataag aacacgttgc aaccacacct
    11701 cggctttgtg gatttccacc gacaataaca ggtcttcctt ttaatttttt attatctctt
    11761 tgttctatag atgcataaaa agcgtccata tctatatgaa ttatttttct ataatgtttc
    11821 attattatca ccttttaaag tataaataga ttaattttat ttatattact ttttaattat
    11881 accaaactta tgtttctttt tggtattttt tcattatttt ctttatatat tcatattttc
    11941 cataaataaa accatctata atccattaac ttcaatataa tttataaaaa atccttaaaa
    12001 acacttgcaa aaactatata aaagctttac aataggtatt ccaaggaact aaatttaaaa
    12061 ttccttatgg ataacagtat atatatttaa tttatgggat gttttaaaaa caaaagactg
    12121 tcttatgcaa gacagcctac tgattttatt ataattgtaa tggactgctc aaactattac
    12181 tttaaaacag cccatatttt tttatataat tttaactata gtaaagattt tgtgaaggat
    12241 attctgggtc acaagttaca tctataccaa gctttctaaa tgtttgttca tcatctctat
    12301 ttaatatagt agtagaatga gcttgacacc cttttaataa tgctaacttt tccatagcaa
    12361 attgtgcagt tggattagtt acagcacaaa tactaagtgc agttaacacc tcttggcaac
    12421 taagtgaaac atttctacta gctaaagtct ttgtttttaa gtttatgata ggttctaata
    12481 ttacaggtga tattaagtgc atatcatcag atatattggc taaatgttta atggcattta
    12541 atactactgc tgcagttcca ttcattaaat cagaaccctt accagttaat attgtaccat
    12601 cttctaattc taatgcaact acaggacaag tatcattctt gtcagcactt tctttcaatt
    12661 tattactgta gtttctagca ggtaatacaa catttctatc ttctggcttt aagttaagct
    12721 cttccataat cattttcatt ctatcataag cacctttatc aacttgtccc tttttatact
    12781 cacaggcagt tttaaagtat cttcttatta tttcttctat agaagctttt cttattactt
    12841 catcatctat tattccaaag ccaactctat taacacccat atcagttgga gattggaaaa
    12901 ttgattcttt tccagttatc ttttctatta tttttttaag cactggaaat aattccaaat
    12961 ctctattgta gtttactgac atttcaccat acgtttcaag gtggaatgaa tctatcatat
    13021 tcacatcttt taaatcaact gtagctgctt cataagctat attcaaagga tgttttacag
    13081 gtacattcca aactgggaat gtctcaaact tagaatatcc aactacatta cctcttttat
    13141 tttcatgata caactgactt agacaagtac caagttttcc actattaggt cctggtgctg
    13201 tgactactac tattggcttt gtagtttcta tatattcatt tgctccatat ccctcatcac
    13261 taactatagt ctcaacatct gacggatatc cctttgttgg ataatgctta taaactttaa
    13321 ttcctctacg ttctaattta tttataaata cagttgtagc aggttgtcct tcaaatctag
    13381 taattactac actatttaca tctagttcat aacctcttaa atcatctatt aatcttaaaa
    13441 cttccatatc ataagtgata ccaaagtctc ctcttatttt gtttctttct atatctcctg
    13501 catagacaca aattacaact tcaacttttt ctttcatatg ttgtaatact tttatctttg
    13561 cattttcatc aaatcctgga agtactcttt ttgcatgaag gtctcccatc aattttcctc
    13621 caaactctaa ataaagtttg tcaaaattat ttactctttc taagatatat ttagactgct
    13681 cttctaaata ttttttgtgg tcaaatccta ctttcatctt aacacctcac taaattaaat
    13741 ttcttattga taacagtata tttatatata tttttttagt aaaacatctt ataaaaaaat
    13801 gttaatactt tttcactcaa agtaatatta tacaattaag attaattttt ttcaagatag
    13861 ttttatggta aataagatat acctaatata actaataaaa taagaatact atatttttat
    13921 atataagtat aatattctca ccttagcatt aatttatttc aatattttca tatatctttt
    13981 attctaaatt caatactttt ctaatatctt ctataccttg ataaacttct tgtttagaaa
    14041 aattcatagc ttctaattct tcatcagttt tttccataag catagtataa aattctattg
    14101 ctatatactg catattttca ctatttacac tcttagcttt atcaaataaa atttcttctg
    14161 cttcgtttac atttccctga aaaactaatc tttttaaagt tataagtata acttcttctt
    14221 ctgtattata cactaaatct ttttcttcat attcactgta tttttttccc aataaaagtc
    14281 ttgcaaccat aagttttatc tcattgaaaa tcttatccac tatagccatt tttacatctc
    14341 ctataaataa attttatttt ctttttaatt atatcagact ttatcgtaaa aattattata
    14401 tgtaaatgaa ataattcaaa aataaatttt aacaataaaa aaaagaaggt agcgggggat
    14461 ttccgactac cttcaaacac tagattgtga ttggtttggt attttattta tacccaccat
    14521 aagctttatt aaatcatcta aattgatttt tttagcatcc acatcctcca tcaaaaacgt
    14581 caccacattc acaaagtatt aataataata ttaagaaagg tatccatgca cataagtcca
    14641 tttcagccca acaatcttgg aatgctaaga ataagaagaa taatattatt atccaccaag
    14701 cacatccacc aaatccgcct tcaccaaaaa atctatccaa cataatatgt tacctcctag
    14761 tcattttgta tgtggagttt cagctcctaa aatatactat gtttaagggg taatatttgc
    14821 taatgatttt tttaaatttt tttagatatt tttaaaaaaa ttattgattc acactcaatg
    14881 tcttaccatc actattgata actacctctc cattagaagt caaaaatgtt tttatattca
    14941 atctttccaa tatttttaaa acaccttcct ctggttcttt tttctctgaa catgtaataa
    15001 cagcatattg gggtgaaata gactctaaaa atgtttcagt taatttgtca tatcttccat
    15061 gatgaggcat tttaacaaaa tcatatttta aagtatttcc atttataaac tctgacaatc
    15121 tttcttcctc tgcatctcct gcaaataaaa acttatgtgc tccatagctt atattagtga
    15181 ttattgaata atcatttgaa ctctcgtact tgctttttga agccggatgt atgtttattt
    15241 ctgcactatt gattacaata ttcatattat cttttagtag cactggttct atatcagctt
    15301 ttttaagtga atcaatatat tcatcatatt gcttactatc tttcttataa tttgcttgta
    15361 taatattctt tactttaaca ctagataaca cactatctac tccacctata tggtccttat
    15421 ctaaatgagt taaaattaaa tagtcaagtg tatttattcc tttttctttt aacctatcca
    15481 ctatttgttt tccattttta tcttctcctg tatcaatcat tatgactttg ttacctattt
    15541 caaccaatat acagtcagac tttccagtac ttaatatagt agtcttaaat gcagtatttt
    15601 ttgtgctact ctgaatatta ttttgactca catcattttt gtcacttttt aaataagatt
    15661 cattcgttga ttttatctta ctattagtag ttatatttgt tacaagcaat aatattacta
    15721 ttatagataa tgtagtaact aaaaacagga tatcttttaa tactattttc ctcatccaaa
    15781 agctcctata aattttattt attacatatt tatagtatag caaccttaac tctgtaaaat
    15841 acacatataa aaagggcaaa ttttaggcat atttgtggca tgataaaaaa atgactgtac
    15901 caaaacaatt atctttctaa atttacatct tcaaattctt taatcttatc attagataaa
    15961 acactctttt tagtagattg tccatttttt ctatcaaaag tatttttagc ttttacacct
    16021 gataaaaact tacctgctcc attaatacaa ccttcttcac acatcattcc ttctataaaa
    16081 ttaccttcta atcttcccaa tttagccatt gtcattgtct ttttaatttg tcttggacca
    16141 gatactttta taggttcaaa atcaaaacta agcccctttt cagcaatata gttctgtact
    16201 gatttggcaa gtccaccaga tgctccaaat ccccttccaa aaatcgaacc atcatttata
    16261 tcttcatttg aacattttga taaatctata ttaaatgctt caaataatgc cattagttct
    16321 tcaaaagtaa gtacataatc tactgaatct tttaaacttt tctctgttgc ctcactcttt
    16381 ttagctgtac atggacctat aaatacaact ttagcatcag aatctatttt ttttactaat
    16441 cttcctgttg ctaccatagg agacactgta gatgatattc tatctttttc atctgggaac
    16501 atcttctcta tatacttaaa aaatcccgga caacatgaat ttgtcatgta attatctcct
    16561 gattttattc tttcaataag ttcattgctt tcatgaactg taactgcatc tgctccacaa
    16621 gaagcttcta tcatatctac aaatcccagt gattttatag cattttttaa ctgaccatat
    16681 gtaatattac tatctatttg acctgttata gctggagcta ctattgcata catattttct
    16741 ttctttgcaa gtttttttgc aacaggtgct atcaaactct tatcagatat tgctccaaat
    16801 ggacatgctg acatacatgc tccacaattt atacaattat cttcatgtat cattgctttc
    16861 atagtgtttc tattaaaatc caaagctcct gttggacaaa cgcttttaca aggtcttaca
    16921 acttctgata tagcatcata ttgacatgaa cttttgcaca ttccacaagc cttacataaa
    16981 tcatgattta tatatgctct cccatttata taagaaattg ccccaaattt acatgcttcc
    17041 ttacatctat gagccaaaca ccctctacaa agctctgtaa ctgtaaattc atttattgga
    17101 catctatcac atgctgcttc aactatatat ataatttgct catctgtgtc caaactaact
    17161 gaatctggat ttatatctcc atatttacta tttggagtta aacttaatgc aagttgcgcc
    17221 ctttcaagaa ctacttctct ctctttataa acacaacatc tatatctcgc cttttctcct
    17281 ttaattattt catatggaat tttttctata ttttcttttg ttaaattttc ttcaatagat
    17341 agtcttgcta ctgctgttaa aacttcatgt tttaaccttt ttagttgcgt ttcaaattca
    17401 aacataagta atcctcccta taaatcacac ctttttgcta tatttctact attatgtata
    17461 ttgtattttt tatttatttc tataaattat tataacatag gcatatactt tgttaagtta
    17521 tatacaaaat attatatatg taatttttat tttaagtgta tataattttt attttagttg
    17581 ttcaataatt aaatatataa ataaaaatag agttgtccaa aagttaaacc tcaacttaga
    17641 acaactctat taacaatatg tatttttgtt gctttgtatt atctttattt ttctaaattt
    17701 atattactaa aatcttttat ctttgtattt gctaagacac ttttcttagt tgattgagaa
    17761 ttttttctat caaaagtggc tttaaccttt gcacctgata agaatttacc agaaccattt
    17821 atacaaccac cttcacacat cattccttct ataaaattac cttctagctt tcctaaatta
    17881 gccatagtca tagttttctt gatttctctt gaaccagaaa tctttacagg ttcaaaatta
    17941 acaccaatac ctttttcagc aagataattt tctatagctt ttgtaagacc accagcagct
    18001 ccaaaacctc ttccaaaaat tgatgcatca tctatatctt catttgaaca cttagccaag
    18061 tctatatcaa atgcctcaaa taatgccatt atttcttcaa aagtaagtac ataatcaaca
    18121 gctcctttta attctttctt agctgcctca ctctttttgg ctgtacatgg tcctatgaat
    18181 acaactttag catctggttc atgacttttt ataaatctac cactagctat cataggagac
    18241 actgtagaag atatttttcc tacatgattt gggtatgttt tttctatata accaacaaat
    18301 cctggacaac aagaatttgt cataaaagct tctccctttt ccattctttc agcaaattct
    18361 aagctttcat gaactgtaac tgcatctgct ccacaagctg cttctaatac atcaacaaat
    18421 cctagtgatt ttatagcatt ttttatttga ccatatgtaa catttgcttc aacttgacct
    18481 gttatagctg gagctaccac cgcatacatt ttctcttttt gaactaactt tctagctaca
    18541 ggagctatca aactcttatc agatattgct ccaaatggac atgctgacat acatgctcca
    18601 cagtttaaac atttatcctc atgtatcata gctttcatat tttctctatc aaatcctaag
    18661 gcattagttg gacaaacact cttacatggt ctaacaacct ctgagatggc atcatattga
    18721 caagcttttt tacacattcc acaagcctta cataattcat gatttatata ggccattcct
    18781 ccgacatgtg ttatagctcc aaatttacaa gcttctttac atctatgtgc tagacatcct
    18841 ctacaaatat ctgttactgt aaattcattt attggacaac tgtcacatgc agcttccaaa
    18901 atatatataa tttgttcatc ttcttctata tccattgtat ctaaatctat cttatcttga
    18961 cctggttgta accccatact tacatttact ctctcagata ttatagctct ttctttataa
    19021 acacagcatc tgtatgtagg tttctcacct tttattattt catactgaat attatccatt
    19081 gcttctctgg taaggttatc ttcttttcca agtctagcta ttgcagttaa aacatcatgt
    19141 cttaatttgt gtatttgtgt ttcaaattga aacattttaa taccaccccc gtaaatgatt
    19201 ttatacgata ccattataac acaatatgtc cttttattaa tatatacata aaatattata
    19261 tgtttaaatt ttattttatc gaatatatat agtgtttttt tgttttatta tataatatta
    19321 ttgatatttc tgaaatttca ctatttttat ttttactttt caatctaata tataatattt
    19381 tttattatat attttaattt tactttttac ttacatacct ttttgttaca ttttttgata
    19441 ttaaatttat tatctacatt tacattttcg tcaaatttta caatacttct cactgtataa
    19501 tttaacaatt tttattaaat tctaaaagta attaattttt ttaaaaattt tatttgcttt
    19561 tatttaaaat ttatgttata ttaatagaac agacttattt ttttagtttg tattttttaa
    19621 taatgcggag gttttcttat aatgaaaaac ggaatagtaa aatggtttaa caatgaaaaa
    19681 ggatttggat tcatatctgt agagggagaa gatgatgttt tcgtacattt ctcagctata
    19741 caaaatgatg gatacaaaac tttagaagaa ggcgaaaagg ttagctttga tataactcaa
    19801 ggtaatagag gacctcaagc tgaaaacgta aatagaatat aattatttaa tttaaatata
    19861 tatattttag aaaaggagct atcgagggct cttttttgtt gtttattata tttattttta
    19921 attacaaaaa taagaagtta ctttagaaaa gcgtcaaact taaaataact tcttatatta
    19981 taatatatac tatttatata aaatactcta tttaatttta tcagatatta ttaagcataa
    20041 ctctcttaag attttacaag ctataactgt cgatacacct gttgtatcgt aatcagggct
    20101 taactctaca atgtcacaac caactatatt tatattagag ttttttataa tcttaaaaat
    20161 ctcttgaaac tctctataat ttacaccacc aggttctggt gtacctgttc ctggaaacac
    20221 agatgcatcc aatacatcta aatctatagt tagataaata ttctttccat ttagcatatt
    20281 aactatattt tcaaacgtat ctattcctcc tatttccatg tatgtgtgtt tttcttctgt
    20341 agcaaattta aattcttctt ttgtcccaga ccttatacca aattgaaata ttttattatc
    20401 acctacaata tcccatattc ttttaattac tgttgcatga gaatttttac tattattata
    20461 ttcttccctc aaatcagtat gggcatcaaa atgaattaca tatatatcat tatacttttc
    20521 atgtactgct ttaaaggctg gtaatgtaac taaatgctct cctccaatca taaagggtac
    20581 ctttgaatct ctaacaatct tatatgtctc ttgatagatt tcttttaaga cttgttctgt
    20641 acttccaaca ctaatttcta aatctccata atcacatata ttataatcct ctaaatccaa
    20701 atctaaaaaa ggactatatg tctcaagacc gtaaaattct tttctcatag agctacttgc
    20761 aaatctagcc ccaggtctgt tagaagtagt tccatcaaat ccaacaccaa atactataag
    20821 attagattct tcataatttt tgttcatact cataaaagtg ttcatatgat agaaattatt
    20881 cttcatccag catctccttc acgtattgag gtaacataaa cgaacctaaa tgtatatcac
    20941 tattataata tttagttttt aaacttaatt tctcccatcc atttctatct tgatttttta
    21001 ctggattaag tttttttgaa gcaaatccaa acaaccagtg accagatgga tatgttggta
    21061 tatgtgcttg ataaacttct gatattggaa atatctgttt taacttttta ttagctcttt
    21121 tcatctcttt agcattaaag tcaaaatacg gactttcact ttgatttact aaaattccat
    21181 catcagtcag tattctataa caatccttat aaaaatcagt tgagaaaagc ccttcacctg
    21241 gtcctattgg gtcagttgaa tctactatta tcaggtcata tgatttatca tgtgcacact
    21301 taacaaaatt aacaccatct tcaaaatata aactcactct tttatcatct aatttacatg
    21361 agcatatatc catatactct ttagatacat ctacaaccat tttatctatt tcaaccatgt
    21421 caatcttttc tatttgagga taacggctta attctcttac tgtacctcca tcacctccac
    21481 ctatgactaa tactttcttt atgtttaaat ttgtagccat aggaacatga gttatcatct
    21541 catggtatat aaattcatct ttatatgtta ccatcattaa tccatctatt gttagaaatt
    21601 ttccaaattc atcactatca aacacatcta tcctttgaaa ttgagacttt ccttcaaata
    21661 gatgtttatt tacttttata gaaaatctta cattttctgt ccattcttct gtataccaaa
    21721 gttccatata ataatcctca ctttctcttg tatgttttat actgcttctg gtttatgagt
    21781 aatcttacct aaatcttttt ttgaatattt tttaattttt gtagttaagc ctcttggaat
    21841 ttctgtagac tcgcttcttt cagactttaa aagttgctct aataattcaa aacttttcca
    21901 tgggtctata tcaccacaag taaaaaaatc tgctgctgca taaccatatt caggccatgt
    21961 atgtattgtc aaatgagatt cagcaattat aacagctcct gaaacccccc aaggattaaa
    22021 atggtggaat acagaatcca caattgtcgc ttttgcattt aatgctgaat cattcataaa
    22081 attttctata agacgtgggt ctttaagtac gttttcatca caattatagt attctactag
    22141 tatatgtctt cctagtgttt caaatttcat ttcaacccca actcctttat acttttaata
    22201 agttctattt gataaactct acttaacaaa cacttgccct tgcaaaataa agtaatttat
    22261 tttattacta gtattctatc taagttttta tcttgtacat ctgtaagata tgcattgcta
    22321 ttttttaaga aaataatata ctccataatt tcttctgtta tcaattctcc tggagcaata
    22381 atcggtatac ctggtggata tgccattatt gattctccac aaattctatc tatacatgaa
    22441 ttgatttcta cactttcctt tggtgcataa aaagcttctc ttggattaag tttaataatt
    22501 ggatttattt gaaccatatt aaattcattt aatgttgatt cttttctata aatctttgca
    22561 attatagaaa gtgcctttac taatctatct atatctgatt tatttgttcc aatggacaca
    22621 agtgccagta tattatatga atctcccaat tctacttgaa ttgaaaaatt tttatataat
    22681 aaatcgtata cttcaaatcc agtcaaattt aattctttta catttataca caattttgtt
    22741 tcatctataa atttaacacc tttttgtttc aatatttctg tagataaaac ttttattcca
    22801 tcaattttgt tcaatttaga ttttgcatac ctagatagat ttaatgcttt tgacaattgc
    22861 ttttcaccat tctcaactag attaactcta gcaccatcta tacttgccat aagcaaataa
    22921 gatgctgatg ttgattgtag catattgatt gattgaagaa ccttttcaaa acttacatta
    22981 tctctattca ataagagtgc tgaagattgt gtcaatgctc ctccagtttt atgtatactt
    23041 acagctacca tgtcagctcc caaacttatt gcagaaggag gtaaatctaa atgaaatgga
    23101 aaatgtgcac catgagcttc atcaactaac actaaaacat tatttttatg gcatagttct
    23161 ataatactct caaggtctgc acaaactcca tagtaagttg gattaaggaa aaataatgct
    23221 tttatattac aatctttttc tatctctgtt ctaacttttt caagagtaat atttaaactt
    23281 atacctagtt tttcatcaaa ttctggttgt ataaatattg gtattcctcc acaaagtatt
    23341 aacgcattta ttactgattt atgtatgttt cttggaagta atactttttc acctggtttt
    23401 ataacactca aaatcattgc atgtattgct tgagttgtgc cattcgttat aaagaatgcc
    23461 tcatcagcca tatatgcttg tgctaacaaa tcttgtgcat tttttataat cccatttggc
    23521 gcagatacat tatccatagt tggtgatgaa tttatatcca ttttcatgag actttcacct
    23581 agatatttat ttaatatagc tacacctcta ttttttacat gtccaggcac atcaaaacaa
    23641 gctatatctt tctctgagta ttcctttaac gactctaata atggagcaca ctcatagctt
    23701 aattcaactg catttgcaaa ctcgctatcc atgatttcaa attcatctcc tctctatatt
    23761 caaatatttt taatatttat tatttattat ttctattttt tatttttact tttattaaac
    23821 tcatgatatt tattatatta acatcccagc atttttacaa gtgttttttt gaagttactc
    23881 aaaaacatat acattagcta tatcaatatt tataaacttt ttaatttttt gttgtttata
    23941 ttaatttttt taaaacttag tatttataaa ctttcttatt ttttattatt ttggtttttt
    24001 tacttttttg agttttagta agcttttgag tttactattt ttaatttata agtattttat
    24061 tactaaactt ttatattatt cttatttcac atatcaaaat tcttggttta gttggactaa
    24121 ataattttcc tatttttgta ttttaatttt aatataaaat ttattaaatt ttttaattga
    24181 taaaataaat tctatattta tgatattaaa attctataag ttcattgatt tatatatgtt
    24241 aatttaaata cattattcta tcgtagtgcc ttagagtttt tatttgtatt taaagttaaa
    24301 tttattcttg tttttccttt aatatttaat agtaaaacaa caaaattata aaaaaaataa
    24361 agagaaactt tataaaagct tctctttttt catctctgct ataggtatcg ttttccaatg
    24421 ttctctcaaa attctttcaa cattttctaa atcattctcc ctcatagatt ttactacttc
    24481 aatatgttga ttattgctgt atgtcaacat tttatataac tcatcttcat tatcactagt
    24541 atatgacttt gacataaaat tatatcttaa agagttcaat agatttacta acacctgatt
    24601 atctgattta gttatgtata tatcgtgaaa ttcattactt aattttaaat agctattgta
    24661 atttttatac tttatagaga ccgccatcat ttctgttaat tcttccattt ttactaggtc
    24721 tgattcagtt aaattttgta atgctgaaga accggctaac gcatccaata ctccaattac
    24781 ggaataggtg tctaatttct ccttttcctg aaattctttt actacaaatc ccttattagg
    24841 tattttttct agtaaatttt cacctgctaa cctagttaat gcttctctcg ctggtgttct
    24901 actaactccg atagatttac aaatctcaga ttcagtaatt ctctgattag cttcatattc
    24961 tccattttgg attttctttg atatatagtc atatacttga gttgtaagtg gatctacctt
    25021 cataagacgt tcctccatat taagattata aaagttaaat ctccctacaa ctttggtaga
    25081 gagttcagtt ttatcttact aatttatata atatcacaat tatgatattt tttatacttc
    25141 tttgtaatat tttgaaatac taaaattctt atgataattt atacaaaaaa agtagaaaaa
    25201 gttttgataa aacaaaactc tttccacatt cgccttattt tctatttatt tttctaaaaa
    25261 aagatattta atttactcta aaaattctaa atgatttaga gtctaaatct actttcaact
    25321 cattattttc aatttgtata gtactatttg catttttaag actatacttt ataggtattt
    25381 cctcaataaa actactctct atacataatg atatactttc cacattttca cttctattta
    25441 ctaaaattaa cattctatca ttattttcat taactctaat aaaagaaaat acagaatcat
    25501 tattagtata tataaattct gtatttccag aacttaaaag tttattctta tttctaattt
    25561 ttataacatc tctataaaaa ttaagcatat cttcgtcttc atttttccat ggataagttc
    25621 ttctattatc tggatcaaca cctccacaaa gacctgcttc atctccataa tatatatatg
    25681 gtactccttc aaatgtcatc tgtatagcta cagcaagctt cactaaatct acatcatcat
    25741 ttaattccgt tttagctctt gtaacatcat gtgttccaat taaatttaaa ttagacctaa
    25801 atgattcttt tggataattt tctttaatct gcatatatct attacaaagt tcatatgaat
    25861 taatttctcc ttttaagaaa gaaaacatgt tatttctaaa aggataaccc ataacagaat
    25921 ctaattcttc acctaataaa taacttcttc tctgaccata gctaatctta tttgatgcat
    25981 cttcccatac ttcacctata agaattgagt catcatctgc cttttttaat tcctttttta
    26041 actctcttat aaacttagtt ggtaattcgt ctgcaacatc tagtctccag cccttaatac
    26101 ccatatttac ccatttattt ataacgctat cattgtcata tattatataa tccatatagc
    26161 tatgttctaa ttcatttata tttggcaatg tcttaacatc ccaccaactt ttatattttt
    26221 gaggaaattc ttcaaacata taccatgaat aatatggaga ttctttagat tgatatgctc
    26281 ctaaactatt atagtttcca tacatattaa aatatttact atctgctcca gtatgactaa
    26341 aaactccatc taatactatt gatatacctt tttcttttgc tttatcaata agttctttaa
    26401 atgtatcttc atctccaaac attgggtcaa tttttttata gtcacctgta tcatacttat
    26461 gattactaga ggcttcaaat atagggctta gatatagtat acttacacct aattttttta
    26521 aatatccaag tttattgatt atccctctta aatttccacc atggaaatcc catcttataa
    26581 catccccttg actatccttt atgtacatag gtgtatcttc ccaatttcca tatatgaaac
    26641 tatttttttt aggattatca accttaccac tacgatttcc attattgaat ctatctacaa
    26701 atatatgata aagtattcct tctttgtacc atgttggtac tttaaaatct ttataaacag
    26761 tgatttgata tttattaata tcactataat tatattcaca agaatatcca ttatctctgt
    26821 tttttccata aaataaagtc tttatttgac catccatttc aacatcaact ttaaagtaat
    26881 aaaaataaac acctgtacca tcaaactttt caatcttacc acaaaagcac tttccaagtt
    26941 tatcattatc taattcttca tttaaagtta ttgttctcat ttcttcttct ctatttattt
    27001 ctagggaaat agacttgata ttgtatccct catttacttt aacacaaatg gtcaattcct
    27061 catcaaattt taaagctcca aacggagcct taaaattttt atcccaagag ttatattcta
    27121 ttacactttg catttaatca caccctttta aaccaaatgt cttttgcata ctcagaaatt
    27181 gttctatcag aagaaaaaaa tccagagtta gctatattaa ctaaagacat tttattccat
    27241 ttttctttat ctctatataa cctgtttata tctgcttgag cttgaccata attttcaaaa
    27301 tctctcaata caaaatactc atcattgtaa gtagtgagtg aattataaat acttcttcct
    27361 tcttcgccaa gattaggaat aaatccattt attaaatcat ctactaccct ttttatatcc
    27421 atattagaat gatataggtc aagtgaagaa tatccaccat atttattata atttagtact
    27481 tgttctgcac taagtccaaa taaaaacata ttttctttac caacctgttc acatatctct
    27541 acatttgcac cgtcaagtgt agccaaagtt atagcaccat tcatcataaa cttcatatta
    27601 cttgttccag atgcttcctt agttgtagtt gaaatttgct cactaacatt tgctgctggt
    27661 attattattt ctgctaatga tactccataa ttctctaaaa atactacttt taacttgtcc
    27721 tttactctaa catcattatt tatagtactt gcaacagaat ttataaattt aattatacat
    27781 ttagccaaat aatatcctgg tgctgctttt gcgccaaata taaaagttct tgggtcaaga
    27841 ttcaaattag gattatcaag taattcatga tacatatgaa gtacattaaa tatattcagt
    27901 aactgtcttt tatacgcatg caatctctta acttgaacat caaatatcga acttgggtct
    27961 acatttaaat cgtatttatc ttttataaac tttgctagat ttgctttatt attgtatttt
    28021 atatcatcaa gtctttgtaa aacagaggaa tcatttttaa atttttcaat attttttaat
    28081 tgtaatgtat ctttcttcca tgaatcacca attaaatctg taattaaatt gctaagttga
    28141 ggatttgaac ttataagcca tcgcctatgt gcaattccat tagtcttatt attaaattta
    28201 tttggttcat cttgatagaa atcttttaat acctctgttt caagaatatg agtatgaagc
    28261 ttagcaactc catttacact atgacttgtg acaatacata agtttgccat attgatattg
    28321 tcacagtcta ttatactcat tctttttatt ctatcttggt catatccttt attatttaat
    28381 tcctctacat atcttctatt tatttcttct attatcatat aaattcttgg taatagctct
    28441 ttcatcatac ttacaggcca tttttccatt gcttctgaca ttatagtatg attagtatag
    28501 gacactgttt ttttagttat ttcccatgct tcatcccaag ataaattttc ttcatcaagc
    28561 aaaattctca taagttctgg tatgcataag gtaggatgtg tatcatttat atgaattgct
    28621 accttatcga ataaattatt aatatttaat ttgttctttt tatattttct tattatatct
    28681 tgtatccctg cacttacaaa aaagtattct tgtttcaacc tcaacaactt acctgcatag
    28741 tttgaatcat caggatataa tacctgtgat atttcctcag tataatactt atgttttaaa
    28801 gcctcttcat agctaccaga ataatttaga gcatttgagg taagttcacc aaaatctctc
    28861 tttggtattt cactcttaaa caatcttaaa gtatttatac attggttttg gtatccgatt
    28921 attggtatat cataaggcat tgccattaca ggaaggtagt ttttatgaat tacttttaaa
    28981 tgactacctt ccttgattaa ttctacctca ccaccaaact tcaccattgt tgcttcattt
    29041 ggtcttacag tttcccatgc atatcgtcct tctgtaagcc agttatcagg cacttctacc
    29101 tgataaccat ttacaaattt ttgttcaaac agaccatatt tataccttat tccatatcct
    29161 tgaccactta tgttcaaaga tgccatagaa tctaaaaagc aagctgctaa tctaccaagt
    29221 ccaccatttc ctaaggctgg gtctacttca gtttcaatta aatcatccaa gttgattcct
    29281 aattcagata aaccttctct aacttcttct tcaatattta aatttaataa attagatttt
    29341 aactgtctcc ctattaaaaa ttctaaacta aaataataaa cttctttttt cttatctaac
    29401 tttgtagcaa cccatttttt tgctatctca tctttcaata gactacataa tacatttaac
    29461 aattgttcat cagtagcttc cttgatagat tgtgcatata agctatacat ttttacttca
    29521 aatttttgct tgaatttctt tttgcttatt gttaccaaaa aaaccactcc tttaatcaca
    29581 tatgtctcta tatgtttcta tatactcctt agctgatttt ttccaactat tatctgtttt
    29641 cattgcattt tcaactaatt taatccattt ttctttgtcc ttaaatactt tgattgcatt
    29701 tttcaaacag aaaaacatct catgagcatt gtaattttta aaactaaatc cattgccttc
    29761 tccagtaaat ttattataag gtgttactgt atctctaagc cctccagtct ctctaactat
    29821 tggtaaagaa ccatatctca tagctaacat ttgacctata ccacaaggct caaataatga
    29881 tggcattaag aacatatcac ttgcagcata tatctgttga gccaatgatg cattaaaagt
    29941 aattctagct gatactttat ctgaaaaatt agaatcgtaa tagttgaaca ttgattgata
    30001 ttgatgttct ccagttccta aaacgacaat ctgtaaatct tctctcataa gttctggcat
    30061 catgtatgct attaaatcaa atcctttttg agaaactaat ctcgaaacta tacctatcaa
    30121 cggaatagat gaatcttttt taagtcccaa tatatcttgt aatctcaatt tattttctac
    30181 ttttttgtca attgagtcta catcataatg aacaaatata tctttatctt ttgcagggtc
    30241 atttaaatca taatctatac catttaatat accttttaat tttcctgact cttttctaag
    30301 taatccatct aattgttcac cataaaaaga tgtttgtatt tcatttgcat aagtaggact
    30361 aacagttata attttatctg caaaatttat accagctttc ataaaattta ttgagccata
    30421 atactctata tctccattat caaagtattt ttgtggtaag tccaatatat catataaaac
    30481 atgcttatca aatacacctt gatattggag attatgtata gtatacatag tttttatatt
    30541 tttatacccc tcaagggttg catatctttc ttttagaatt aatggtaaca tccctgtatg
    30601 ccaatcattt atatgtatta catctggata aaaatcaatt ctacttatag cttctaaaac
    30661 tgcatctgta aagaaagtat atctttctgc atcatctcca tatccataga tactctttct
    30721 ttcatcttct cttctaaaat aaaattcatt atctataaag taaaatttta caccatctaa
    30781 ttccatttct aaaagaccac agtattgatt tctccatcca acacgtacac taaacacagc
    30841 tatttctttt agttgatctt ttaaataact tggtatagtt gaatatttgg gcattattac
    30901 ccttacatct acaccttctt tttttaattc tttaggcaat gcataagaaa catctcctaa
    30961 tcctccagtt tttgcaaagg gccaacactc tgctgttaca taaaaaacct tcattagtca
    31021 ttcccccttc taaaaaattt atttatatat ttgtattctt ttccactaca agaggatatt
    31081 tttcatctcc tcttaattct ttcccatttg atacactcgt atttttatct aatattgcat
    31141 tatatagttt tgcatttgac tttattacac aattttgcat tactattgag ttctctataa
    31201 ctgttccttt ttcaacattt acctttctaa atatgatact atcttttaca gtaccttcta
    31261 ttaaacaacc agatgctata aagctatttt ctactttagc actatctgta taaattgtag
    31321 gtgattcatt cttttctttt gtaaatatct ttctttcaga atataataat tcatctgcaa
    31381 cttctaaatt taataaatct ttgctcattt caaaatatga ttcaacagaa tttacacatt
    31441 ttagatatcc tgtatattca taggctccta ttttcatatt ttctgcttct tcaataataa
    31501 attcttctaa ataacaataa ttgcctttat ttacaatgtt atatatacat tcaacaaaat
    31561 cttcacgctt cataatatat gtctccatac atatgtttgc tctagggaac ttacctaaat
    31621 tatttcctac atttattacc ctattgttac tatctaaatt tagagttaag ttaccagcaa
    31681 aatctttatt tgcattattt acatttttat atattatagt tatatcatta tgagattttt
    31741 tatggtattc caatgcttga gaataattta aattgcatag catatagctt ggagctatta
    31801 aaatatattc ttcttcactc ttttctatat aatctagatt agctaagata gtatatatat
    31861 caccttctct atgtgagtaa gtatttctat acttagtgtt ttctggacta aaaattgata
    31921 ggccaccttt ttttctactt aaatcccaat ctttaccact acctatatgg tctgttaatg
    31981 atctgtattt ttcttttgcg aatattccaa ctttagtgat tcctgaattt atcatattag
    32041 ataaagcaaa gtctattatt ctatatcttc cagctattgg tgtagatgct attggtcttc
    32101 catagttaag tttgtttatt gctgggtctc cttttttgtt taaatttatt atccctaaac
    32161 attcatttct catcttaaag acctccttct attaatacgc atctaggttc gatattttca
    32221 tactcagaaa ctacatttat ttcattattt acattcttta tctgaacatt atcatttata
    32281 gttgcacact cacctatcat agctttttct atagttacat tttcacctat tacaacattt
    32341 ggcattatta ctgaatcaat tactttactt cctttaccaa caacaactcc atgtgataat
    32401 acagagttac ctacttcacc taaaattcta caaccatcag ctatcattga tctatgaaca
    32461 tttgcatttt tacctatata ttgtggaggc attgccatag tatttgtata tatcttccac
    32521 ttagggtctg ctaaatcaag tgtttctgga gattttatta tatccatatt agcatcccat
    32581 agactttgta tagttcctac atctctccag tatcctttaa atggataagc atacattcca
    32641 acattatctt ctaacatttt aggtattaaa ttttttccaa aatcatcata atttatttct
    32701 ggattttttt ctgcttcctt aaaataattt ctcaacattt tccaatcaaa tatgtatact
    32761 cccatagatg ccaaattgct cttaggttca cttggttttt cttcaaattc atagatactt
    32821 gaatcttcat ttgtattcat tatcccaaat cttgaagctt catcccaagg aacttcaatc
    32881 actgctattg tagctttaga gcctttttcc ttatgaaatt ttaacatttt gctataatcc
    32941 atcttgtata tatggtcacc agataatatc aatacatatt ctggattata ggtatctaca
    33001 aagtccatat tttgatatat cgcatgtgct gtaccattat accaatttcc ttctttttca
    33061 ttcataaatg gttgtaaaac atagacacca ccatttattc tgtccaaatc ccaatgactt
    33121 cccatgccta tatgtgaatt taatatcaac ggtctatatt gagttaaaac acctacagta
    33181 tcaataccag agtttgaaca attgcttaac acaaaatcta taattctata cttacctcca
    33241 aatgatacag ctggtttggc aattcttttt gtaaaaacac ctaatcttga accttgccct
    33301 cctgccaaaa tcatagctaa catctctttt ttcatttaat gacactcccc tcatctagta
    33361 attaatattt ataattgcat ttttactttt ataaacttta tcagtcaata aatgtcgtgt
    33421 ataaatgttg cttaaataat accttaataa aaataaaata atatcaacta tacaattgat
    33481 ataatgtata ccccataatg gacttaccga cgtatacatt attttccata agcactatag
    33541 tatttattaa tattcaatta ttggttaaaa aataatacaa attttatttt ataaatataa
    33601 aaacattttt tataaatcct ttgtgtcata tgaacatgct tattttttaa tttgttaaat
    33661 tcgtaatagc aaaatataat ctaatatttg ttaaataatt atccaaagaa aatcaagtaa
    33721 ttattaatca actttatttt ttaattg
//
LOCUS       DBGJFU010000032        33615 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.032, whole genome shotgun sequence.
ACCESSION   DBGJFU010000032 DBGJFU010000000
VERSION     DBGJFU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.032"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(257..826)
                     /gene="yfcE"
                     /locus_tag="ACOIAS_001465"
     CDS             complement(257..826)
                     /gene="yfcE"
                     /locus_tag="ACOIAS_001465"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGZ2701181.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(851..1651)
                     /locus_tag="ACOIAS_001466"
     CDS             complement(851..1651)
                     /locus_tag="ACOIAS_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HGZ2701182.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            1786..2646
                     /locus_tag="ACOIAS_001467"
     CDS             1786..2646
                     /locus_tag="ACOIAS_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701183.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFIYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            2646..3332
                     /locus_tag="ACOIAS_001468"
     CDS             2646..3332
                     /locus_tag="ACOIAS_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HGZ2701184.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            3384..3926
                     /locus_tag="ACOIAS_001469"
     CDS             3384..3926
                     /locus_tag="ACOIAS_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701185.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            4005..4472
                     /locus_tag="ACOIAS_001470"
     CDS             4005..4472
                     /locus_tag="ACOIAS_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HGZ2701186.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            5181..6317
                     /locus_tag="ACOIAS_001471"
     CDS             5181..6317
                     /locus_tag="ACOIAS_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGZ2701187.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            6376..7158
                     /locus_tag="ACOIAS_001472"
     CDS             6376..7158
                     /locus_tag="ACOIAS_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGZ2701188.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            7177..8187
                     /locus_tag="ACOIAS_001473"
     CDS             7177..8187
                     /locus_tag="ACOIAS_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGZ2701189.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            8206..9003
                     /locus_tag="ACOIAS_001474"
     CDS             8206..9003
                     /locus_tag="ACOIAS_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HGZ2701190.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            9101..9946
                     /locus_tag="ACOIAS_001475"
     CDS             9101..9946
                     /locus_tag="ACOIAS_001475"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HGZ2701191.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            10033..11208
                     /locus_tag="ACOIAS_001476"
     CDS             10033..11208
                     /locus_tag="ACOIAS_001476"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGZ2701192.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            11404..12306
                     /locus_tag="ACOIAS_001477"
     CDS             11404..12306
                     /locus_tag="ACOIAS_001477"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGZ2701193.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEDLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            12320..13324
                     /locus_tag="ACOIAS_001478"
     CDS             12320..13324
                     /locus_tag="ACOIAS_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGZ2701194.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(13468..13695)
                     /gene="acpP"
                     /locus_tag="ACOIAS_001479"
     CDS             complement(13468..13695)
                     /gene="acpP"
                     /locus_tag="ACOIAS_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGZ2701195.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            13867..14292
                     /locus_tag="ACOIAS_001480"
     CDS             13867..14292
                     /locus_tag="ACOIAS_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701196.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            14340..14546
                     /locus_tag="ACOIAS_001481"
     CDS             14340..14546
                     /locus_tag="ACOIAS_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701197.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            14728..14907
                     /locus_tag="ACOIAS_001482"
     CDS             14728..14907
                     /locus_tag="ACOIAS_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701198.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            14941..15141
                     /locus_tag="ACOIAS_001483"
     CDS             14941..15141
                     /locus_tag="ACOIAS_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701199.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            15388..16410
                     /gene="ccpA"
                     /locus_tag="ACOIAS_001484"
     CDS             15388..16410
                     /gene="ccpA"
                     /locus_tag="ACOIAS_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HGZ2701200.1"
                     /translation="MKMKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEV
                     IKETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANT
                     YSDKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYT
                     VSTSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKS
                     LIKYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVA
                     GFNDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRES
                     VTERKK"
     gene            complement(16533..16931)
                     /locus_tag="ACOIAS_001485"
     CDS             complement(16533..16931)
                     /locus_tag="ACOIAS_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701201.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            17143..18048
                     /locus_tag="ACOIAS_001486"
     CDS             17143..18048
                     /locus_tag="ACOIAS_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701202.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            18163..18343
                     /gene="ssrS"
                     /locus_tag="ACOIAS_001487"
     ncRNA           18163..18343
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACOIAS_001487"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            18526..19743
                     /gene="cdeC"
                     /locus_tag="ACOIAS_001488"
     CDS             18526..19743
                     /gene="cdeC"
                     /locus_tag="ACOIAS_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HGZ2701203.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(19843..21165)
                     /locus_tag="ACOIAS_001489"
     CDS             complement(19843..21165)
                     /locus_tag="ACOIAS_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGZ2701204.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(21216..22010)
                     /locus_tag="ACOIAS_001490"
     CDS             complement(21216..22010)
                     /locus_tag="ACOIAS_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HGZ2701205.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            22120..23565
                     /locus_tag="ACOIAS_001491"
     CDS             22120..23565
                     /locus_tag="ACOIAS_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2701206.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            23594..23803
                     /locus_tag="ACOIAS_001492"
     CDS             23594..23803
                     /locus_tag="ACOIAS_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HGZ2701207.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            23823..25217
                     /locus_tag="ACOIAS_001493"
     CDS             23823..25217
                     /locus_tag="ACOIAS_001493"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGZ2701208.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            25457..26209
                     /locus_tag="ACOIAS_001494"
     CDS             25457..26209
                     /locus_tag="ACOIAS_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2701209.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            26235..26648
                     /locus_tag="ACOIAS_001495"
     CDS             26235..26648
                     /locus_tag="ACOIAS_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2701210.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            26652..27395
                     /locus_tag="ACOIAS_001496"
     CDS             26652..27395
                     /locus_tag="ACOIAS_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HGZ2701211.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            27395..27865
                     /locus_tag="ACOIAS_001497"
     CDS             27395..27865
                     /locus_tag="ACOIAS_001497"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2701212.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            27882..28640
                     /locus_tag="ACOIAS_001498"
     CDS             27882..28640
                     /locus_tag="ACOIAS_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2701213.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            28624..29466
                     /locus_tag="ACOIAS_001499"
     CDS             28624..29466
                     /locus_tag="ACOIAS_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2701214.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(29626..30531)
                     /locus_tag="ACOIAS_001500"
     CDS             complement(29626..30531)
                     /locus_tag="ACOIAS_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HGZ2701215.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            30893..31627
                     /locus_tag="ACOIAS_001501"
     CDS             30893..31627
                     /locus_tag="ACOIAS_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701216.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            31838..33019
                     /locus_tag="ACOIAS_001502"
     CDS             31838..33019
                     /locus_tag="ACOIAS_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGZ2701217.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKFSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            33003..33575
                     /gene="mobB"
                     /locus_tag="ACOIAS_001503"
     CDS             33003..33575
                     /gene="mobB"
                     /locus_tag="ACOIAS_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HGZ2701218.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
BASE COUNT        13209 a         3647 c         5949 g        10810 t
ORIGIN      
        1 actaagttat atttcagtat aatatataat gaaataagta aactaaatta tactccaata
       61 taatatataa tagaaacaag taaactaagt tatatttcaa tatagcatat aataaaacaa
      121 gtaaaccaga ttctatttaa ttagaagttt aaggtattgt acttaaactt ctaattgaga
      181 tagaaataaa atggtttact tgtctttatt atgaatttaa taaattaaag cttattttat
      241 ttttattaaa aatttactaa tcattgatat ttattatatt tccagtattg atgtcaatta
      301 agtttatatc aagttcaagc tcatcctcat catcagtttt tatgatatct ataattgcga
      361 cagaatggct tccatctttt gggatagaag tacttcctgg atttattact attaaattat
      421 catccatgta tagttttttt acatgagtat gtcctaaaac taatatgtca ccacccattt
      481 tctttgcttt gtcaataatt tcttcttctg attctatata accatgattc aatataattc
      541 taatttcacc aaattggctc atgacataag gactctgaat aggatgttct ataaccatct
      601 ggtcaacatc tgcatcacag ttccctttta caatcacaat gttttctaat ttatttaatg
      661 tctcaataaa ttttttagga ttatatcctt ctggaatatc atttctaggt ccatgatata
      721 agacatctcc accatgtaat aaaacatcac aatcagataa tacatttaac gctttttcaa
      781 agtaaagtaa gctaccatga gtatcactca ttattcctat tttcatatta aaaatcctcc
      841 taagtacagt ctataaaaat ttttcagata ctctatgcca aaactcatag tttgacattc
      901 taagtaattt tatttttgca tcagatatac taacttttat attttcgatt tgtctaaatc
      961 tgtgctcaac tccatcaacg acaatcaata cagaatcttc aaatctatat tctggtgcaa
     1021 tctttataac agattcattt gaacatatta tacttgatgt aaagcaccta tatgcattgg
     1081 tatttatcgg atgtaaaggt gtaatctgca taagttcaag atttatatca acaatactac
     1141 ctccagcaga atagttatag gctgttgaac ctgtggaagt agaaataatc ataccatctc
     1201 cactaaaatt ttgtatatgt ttattatcaa ggcttaaatt taggtgtata gtcctagact
     1261 tgtctcctct tataacaacc tcatttacag caagcatatt agaaccacta gtagttgtat
     1321 atacttccgc attaagaagt gacatttctt gaataatata atcttttttt gtataagctt
     1381 caataaagct atcaatctta tctggtaaaa tatcaggaaa aaaccccaga tgacctgtat
     1441 ttattcccat tatgggaatc tcaggaaaat cgaaatctct aactgttctt aaaaaagagc
     1501 catcaccacc aattgatata ataagctctg tgtctggata aatatctgaa tacacttcaa
     1561 agccagcatt gataagtttt ctagttaaga gttctttagt ctcaagggac ttatttaatt
     1621 ggtttgtatt tatagttata attcttttca tatattcacc tcttcatact ttgtattata
     1681 acatagtttt ataattctta atttataaat ttataaatga cctttgtagt gaatgtgtta
     1741 aaatatgtat aggtgggtat aaaaaaatgc ataagaggtg aagatgtgtt tagcataagg
     1801 aatatcacaa aaaaattagg aaaattcaag ttaaataata taaacttaga gttaaaggaa
     1861 ggggatattg ttgggattat aggaccaaat ggttcgggaa aaacgacttt aataaaaatt
     1921 ataatgggga ttattgatgc agatgaaggt gaaattgaac tttgtaatga aactattgaa
     1981 aattctccaa taagctttaa aaacaatatt ggttttatat atgattcttt acaattttat
     2041 ccgcatttaa aggttaagga atttagaaaa atagtttctt tattttataa gaattttgat
     2101 agagaaaggt ttgatgaata ccttaataag tttgatatag aagaaaatat gcatattgaa
     2161 aatttatcta aaggtcaatc tgaaaagctt atgttatcaa gtgcacttag tcacaatgct
     2221 aagttattga ttttggatga accaactgct gggatagacc caattgtaag aactgaaata
     2281 atgcagtact tacaagattt tgtgaaaaat ggaagtagta gtgtaataat ttctactcat
     2341 aatactgata atttaattaa aattgctgat tatcttgttt ttataaatag aggaaatcag
     2401 atttttactg ttaagaaaga attaatagaa caagaatata agataattag ggcaaataaa
     2461 gcagagttag aagcaattaa agaaagcata gttggtgtga aagagtataa atattataat
     2521 gaggcacttg ttaaagttgg agatagcttg aaagtaaaaa gcctattgat agaaattgat
     2581 aagcataagg tcaaaaatcc tactatagaa gaactaatgt attattatgt aaatgaggtg
     2641 agatgatgtt caatttaata agaaaagatt taataatagg gattagttca gatggtatcc
     2701 gtaatttaaa gtacatattg ctattttttg tgttttattt tttccttaat tcaatttcat
     2761 attatacagt aagtattgtt gttagttatt tgatatttat aaatacgttt gagtgtgatt
     2821 atgaaaatga ttcaaggatt tttataagga gtatgccagt tagtatagaa gatatagttt
     2881 actcaaaata tttattaggt gtaggattaa taatttctgt aacaataatt gtgtccttac
     2941 taagtaaatt gacatcacta gtgttcttta gaaatatggt tttaaatgat gtattttttt
     3001 ctgtaaatat atttttagca attttgacga ttttactgcc tttaattttt aagtttggat
     3061 atggaaaaat gaaagtttgt ggttttatag taagtatttt aatttacttt gtatatggaa
     3121 gtcttttgaa gatgattagt atgattgtat atcaagtaaa gcattttaat tatagtaaag
     3181 ttggaggagt atatctttca aattatgtaa ctgatatagc taatactaag tatataaatt
     3241 tatatagtat gacattttta acaataataa tttttataat atcaatgtat ttttctatta
     3301 agatttcaaa aaagaataag tttaattatt aaaattttga ttaatattat agcacaatag
     3361 cacaaaatat aaggagacta aaaatgaaga agtttataaa agtttcattt tttattttgg
     3421 ggatttttac actattcata gctataaata aattagtata tagagatatg gtaagtgtca
     3481 aagattatga aataaatcat ttagaggaaa ttgtttttga ttttgatagc aggagaagta
     3541 gagatgttgg tactattgta agatttaaaa taagcaataa tagtaaatat agttataaat
     3601 taaacagtgc taaaatgaag tttgaaaatt gtgtggaaaa taaaaaggga atgaataagt
     3661 gttctgcata tgtaaatgta gatttattta atgaaaaaaa ttctataact gaaagaactc
     3721 tgaaatatgg aataaaacca aataatcaag gttatgttga ttttgtaatt ccaaaaggac
     3781 taaattttga ttataggtac tttaatgaat ctggtatgag tgtagagtat aaaggagaat
     3841 atattgtcaa tattccaatg gcaaaaggac tttatctagt agttggaaaa aataatgata
     3901 catggaatgc taatatcatg gaataatttg aaaaataaat caattataga tataattaag
     3961 aataatatga aatttataaa agttcagact aggagggata tttaatgtca ataagtaatg
     4021 taagagagta ttttaaacaa tttggcaagg aagacagtat attagaattt gaacaatcta
     4081 gtgctactgt ggaacttgca gctgaagcag ctggggttat tccagcaaga atagctaaga
     4141 cactctcatt taaaatagga gatgatgcaa ttttaattgt gacagctggt gatgctaaga
     4201 ttgataataa gaagtataag gctgaattta attgtaaggc taaaatgttg acaccagaag
     4261 aagtcttgga gtttactgga catgctattg gtggagtctg tccatttggt ttaaaaaatt
     4321 ctataaaagt ctatttagat gattcaatga aaagatttga tacagtattt cctgcatgtg
     4381 gcagtagtaa ctctgcaata gaacttacat gtgaagaaat ggagaagttt tctaagtgtg
     4441 aaaaatgggt agatgtttgt aagaattggt aaaaaaatat agtaaactat agtaggaatt
     4501 agtaaaaaac tacctctgta tacaatctat acagaggtag tttttttgtg taaatattta
     4561 taaatctaag aggaaaatct aatttaaact aataatccaa aataaagtta aaaaattctg
     4621 attttttttc gaaacaataa aaaaatgaca aaaaagtgga aaaaatgtaa taaatattgt
     4681 taaaaaaata attagaaatt attgaaaaat tatcctttga gtgatactat tatagatgta
     4741 agatatttaa aaataaaaaa tagtcagata aaaatacgat tatttagctc tattaaaata
     4801 atttttatac gataaaaatc ttctgtacat ggctaaaaaa cagatgtaca gacaatattt
     4861 aatgtttaat ataccctaat ataaatatcg taaacaaatt attttatctg taaccagtgt
     4921 gaaagaaatc ttattctaac ttgaaatttc acaaaattcc aaaaagcgag atttttttta
     4981 agtatagttg attttaaatt aacaatttat gattaaatta atgtcataat tggttagtat
     5041 aatcacatat aaatatagag cgtagtttga ttaaggttat aaatttctcg acgtatattc
     5101 aaatacctta ttatagatgt tgccgagatt ttaattaata aatgaaaata aaatatttat
     5161 aaaatactga ggaggctatt atggatttaa attctaaaaa atatcagatg cttaaagagc
     5221 tatatgtaag cttcgctgaa aatgaagtta aacctttagc aacagaactt gatgaagaag
     5281 aaagatttcc ttatgaaaca gtggaaaaaa tggcaaaagc aggaatgatg ggtataccat
     5341 atccaaaaga atatggtgga gaaggtggag acactgtagg atatataatg gcagttgaag
     5401 aattgtctag agtttgtggt actacaggag ttatattatc agctcataca tctcttggct
     5461 catggcctat atatcaatat ggtaatgaag aacaaaaaca aaaattctta agaccactag
     5521 caagtggaga aaaattagga gcatttggtc ttactgagcc taatgctggt acagatgcgt
     5581 ctggccaaca aacaactgct gttttagacg gggatgaata catacttaat ggctcaaaaa
     5641 tatttataac aaacgcaata gctggtgaca tatatgtagt aatggcaatg actgataaat
     5701 ctaaggggaa caaaggaata tcagcattta tagttgaaaa aggaactcct gggtttagct
     5761 ttggagttaa agaaaagaaa atgggtataa gaggttcagc tacgagtgaa ttaatatttg
     5821 aggattgcag aatacctaaa gaaaatttac ttggaaaaga aggtcaagga tttaagatag
     5881 caatgtctac tcttgatggt ggtagaattg gtatagctgc acaagcttta ggtttagcac
     5941 aaggtgctct tgatgaaact gttaaatatg taaaagaaag agtacaattt ggtagaccat
     6001 tatcaaaatt ccaaaataca caattccaat tagctgatat ggaagttaag gtacaagcgg
     6061 ctagacacct tgtatatcaa gcagctataa ataaagactt aggaaaacct tatggagtag
     6121 aagcagcaat ggcaaaatta tttgcagctg aaacagctat ggaagttact acaaaagctg
     6181 tacaacttca tggaggatat ggatacactc gtgactatcc agtagaaaga atgatgagag
     6241 atgctaagat aactgaaata tatgaaggaa ctagtgaagt tcaaagaatg gttatttcag
     6301 gaaaattatt aaaatagata aatattaaaa tatacacaat aggtcgatat taaaatagat
     6361 atggaggaag gatttatgaa tatagtcgtt tgtataaaac aagttccaga tacaacagaa
     6421 gttaaactag atcctaatac aggtacttta attagagatg gagtaccaag tataataaac
     6481 cctgatgata aagcaggttt agaagaagct ataaaattaa aagaagaaat gggtgctcat
     6541 gtaactgtta taacaatggg acctcctcaa gcagatatgg ctttaaaaga agctttagca
     6601 atgggtgcag atagaggtat attattaaca gatagagcat ttgcaggtgc tgatacttgg
     6661 gcaacttcat cagcattagc aggagcatta aaaaatatag attttgatat tataatagct
     6721 ggaagacagg cgatagatgg agatactgca caagttggac ctcaaatagc tgaacattta
     6781 aatcttccat caataacata tgctgaagaa ataaaaactg aaggtgaata tgtattagta
     6841 aaaagacaat ttgaagattg ttgccatgac ttaaaagtta aaatgccatg ccttataaca
     6901 actcttaaag atatgaacac accaagatac atgaaagttg gaagaatata tgatgctttc
     6961 gaaaatgatg tagtagaaac atggactgta aaagatatag aagttgaccc ttctaattta
     7021 ggtcttaaag gttctccaac tagtgtattt aaatcattta caaaatcagt taaaccagct
     7081 ggtacaatat acaatgaaga tgcgaaaaca tcagctggaa ttatcataga taaattaaaa
     7141 gagaagtata tcatataatt taagggggga taaagaatgg gtaacgtttt agtagtaata
     7201 gaacaaagag aaaatgtaat tcaaactgtt tctttagaat tactaggaaa ggctacagaa
     7261 atagcaaaag attatgatac aaaagtttct gcattacttt taggtagtaa ggtagaaggt
     7321 ttaatagata cattagcaca ctatggtgca gatgaggtaa tagtagtaga tgatgaagct
     7381 ttagcagtgt atacaactga accatataca aaagcagctt atgaagcaat aaaagcagct
     7441 gaccctatag ttgtattatt tggtgcaact tcaataggta gagatttagc gcctagagtt
     7501 tctgctagaa tacatacagg tcttactgct gactgtacag gtcttgcagt agctgaagat
     7561 acaaaattat tattaatgac aagacctgcc tttggtggaa atataatggc aacaatagtt
     7621 tgtaaagatt tcagacctca aatgtctaca gttagaccag gggttatgaa gaaaaatgaa
     7681 cctgatgaaa ctaaagaagc tgtaattaac cgtttcaagg tagaatttaa tgatgctgat
     7741 aaattagttc aagttgtaca agtaataaaa gaagctaaaa aacaagttaa aatagaagat
     7801 gctaagatat tagtttctgc tggacgtgga atgggtggaa aagaaaactt agacatactt
     7861 tatgaattag ctgaaattat aggtggagaa gtttctggct ctcgtgccac tatagatgca
     7921 ggttggttag ataaagcaag acaagttggt caaactggta aaactgtaag accagacctt
     7981 tatatagcat gtggtatatc tggagcaata caacatatag ctggtatgga agatgctgag
     8041 tttatagttg ctataaataa aaatccagaa gctccaatat ttaaatatgc tgatgttggt
     8101 atagttggag atgttcataa agtgcttcca gaacttatca gtcagttaag tgttgcaaaa
     8161 gaaaaaggtg aagttttagc taactaattt cttggggagg gtattatgag tacaagtgat
     8221 gttaaagttt atgagaatgt agctgttgaa gtagatggaa atatatgtac agtgaaaatg
     8281 aatagaccta aagcccttaa tgcaataaat tcaaagactt tagaagaact ttatgaagta
     8341 tttgtagata ttaataatga tgaaactatt gatgttgtaa tattgacagg ggaaggaaag
     8401 gcatttgtag ctggagcaga tattgcatac atgaaagatt tagatgctgt agctgctaaa
     8461 gattttagta tcttaggagc aaaagctttt ggagaaatag aaaatagtaa aaaagtagtg
     8521 atagctgctg taaacggatt tgctttaggt ggaggatgtg aacttgcaat ggcatgtgat
     8581 ataagaattg catctgctaa agctaaattt ggtcagccag aagtaactct tggaataact
     8641 ccaggatatg gaggaactca aaggcttaca agattggttg gaatggcaaa agcaaaagaa
     8701 ttaatcttta caggtcaagt tataaaagct gatgaagctg aaaaaatagg gctagtaaat
     8761 agagtcgttg agccagacat tttaatagaa gaagttgaga aattagctaa gataatagct
     8821 aaaaatgctc agcttgcagt tagatactct aaagaagcaa tacaacttgg tgctcaaact
     8881 gatataaata ctggaataga tatagaatct aatttatttg gtctttgttt ttcaactaaa
     8941 gaccaaaaag aaggaatgtc agctttcgtt gaaaagagag aagctaactt tataaaaggg
     9001 taaaaatcta attaaaaata tatttgctgt taactagatt aaatagtatt ttaatctagt
     9061 taacagaata taaatactaa aataacagga ggttataact atgaaattag ctgtaatagg
     9121 tagtggaact atgggaagtg gtattgtaca aacttttgca agttgtggac atgatgtatg
     9181 tttaaagagt agaactcaag gtgctataga taaatgttta gctttattag ataaaaattt
     9241 aactaagtta gttactaagg gaaaaatgga tgaagctaca aaagcagaaa tattaagtca
     9301 tgttagttca actactaact atgaagattt aaaagatatg gatttaataa tagaagcatc
     9361 tgtagaagac atgaatataa agaaagatgt tttcaagtta ctagatgaat tatgtaaaga
     9421 agatactatc ttggcaacaa atacttcatc attatctata acagaaatag cttcttctac
     9481 taagagacca gataaagtta taggaatgca tttctttaat ccagttccta tgatgaaatt
     9541 agttgaagtt ataagtggtc agttaacatc aaaagttact tttgatacag tatttgaatt
     9601 atctaagagt atcaataaag taccagtaga tgtatctgaa tctcctggat ttgtagtaaa
     9661 tagaatactt atacctatga taaatgaagc tgttggtata tatgcagatg gtgttgcaag
     9721 taaagaagaa atagatgaag ctatgaaatt aggagcaaac catccaatgg gaccactagc
     9781 attaggtgat ttaattggat tagatgttgt tttagctata atgaacgttt tatatactga
     9841 gtttggagat actaaatata gacctcatcc acttttagct aaaatggtta gagctaatca
     9901 attaggaaga aaaactaaga taggattcta tgattataat aaataagaat ttgggaatta
     9961 aaagtttaaa taaaattgtt taaaaaacaa tttcggatat atgaaaaatc taatttaatg
    10021 ggggtaatga atatgagaga agtagtaatt gccagtgcag ctagaacagc agtaggaagt
    10081 tttggaggag catttaaatc agtttcagcg gtagagttag gggtaacagc agctaaagaa
    10141 gctataaaaa gagctaacat aactccagat atgatagatg aatctctttt agggggagta
    10201 cttacagcag gtcttggaca aaatatagca agacaaatag cattaggagc aggaatacca
    10261 gtagaaaaac cagctatgac tataaatata gtttgtggtt ctggattaag atctgtttca
    10321 atggcatctc aacttatagc attaggtgat gctgatataa tgttagttgg tggagctgaa
    10381 aacatgagta tgtctcctta tttagtacca agtgcgagat atggtgcaag aatgggtgat
    10441 gctgcttttg ttgattcaat gataaaagat ggattatcag acatatttaa taactatcac
    10501 atgggtatta ctgctgaaaa catagcagag caatggaata taactagaga agaacaagat
    10561 gaattagctc ttgcaagtca aaataaagct gaaaaagctc aagctgaagg aaaatttgat
    10621 gaagaaatag ttcctgttgt tataaaagga agaaaaggtg acactgtagt agataaagat
    10681 gaatatatta agcctggcac tacaatggag aaacttgcta agttaagacc tgcatttaaa
    10741 aaagatggaa cagttactgc tggtaatgca tcaggaataa atgatggtgc tgctatgcta
    10801 gtagtaatgg ctaaagaaaa agctgaagaa ctaggaatag agcctcttgc aactatagtt
    10861 tcttatggaa cagctggtgt tgaccctaaa ataatgggat atggaccagt tccagcaact
    10921 aaaaaagctt tagaagctgc taatatgact attgaagata tagatttagt tgaagctaat
    10981 gaggcatttg ctgcccaatc tgtagctgta ataagagact taaatataga tatgaacaaa
    11041 gttaatgtta atggtggagc aatagctata ggacatccaa taggatgctc aggagcaaga
    11101 atacttacta cacttttata tgaaatgaag agaagagatg ctaaaactgg tcttgctaca
    11161 ctttgtatag gtggaggaat gggaactact ttaatagtta agagatagtt ttagattata
    11221 atatttacaa attaaatatt taattatgaa tttaacctgt aagaataact atttgttatt
    11281 cttacaggtt tttatagtta aaaataatta ttatctatta aacaaaaata gtgtaaaata
    11341 tataatatca tgattttgaa gatataaaaa agtaggaaga tataaaagaa agtaggtaaa
    11401 agtttgttta aaaaagagaa tcaaagatat aatcttatat cgtatacaaa tgaagaagaa
    11461 atgactttaa aagaagtttt attggacaag ctaaatttct cagttaggtc attatcaaaa
    11521 atgaagaggg aaaagagtgt tttagtaaat ggagtatata aaaaaccgag tttaaaggtg
    11581 tatagtggtg atttaataga agtaaaaata gacgaggaaa aagcaaattt tgaacctcaa
    11641 gatttaaatt tacagataat ttatgatgat tttgatataa ttatggtgaa taaacctccg
    11701 tttatggtag ttcacccaac aaaaagccac tatgataaaa caatagcaaa tggtattagt
    11761 tactatatag ataatcaaaa agaaaatgta aaaataagat ttgtaaatag gttagatatg
    11821 aatacttcag ggttagttat agtagcaaaa aatgcttatg cacatcatac tttgtctaca
    11881 gcaatgagtg aaaataaagt tgaaaaaaag tatataacag tagttgatgg aattataaaa
    11941 gaaaatgagg gaactataga tgagccaatt tatagaccta cagaagattc aataaagagg
    12001 ataatagatg aaagaggaca gtcatctgtt actcactata aagtaataga aagactagaa
    12061 aatgcaactg tattagaagt aagcttagaa actggtagaa cacatcaaat aagagtacat
    12121 atggctcata taggtcatgg aataataggt gatgagttgt atggttatgt agatgaagat
    12181 ctaataaata ggcaagcact tcatgcatat aagctagagt ttgaacaacc aagaacaaaa
    12241 gaaaaattga aatttaaagc agatatacca gaggatatga aagagttaat atcgaaatta
    12301 agataatggg gtgatatata tgataataca taaatttata atacatgttt tggacaaaaa
    12361 tagtgatgtt ccaatattaa atgactttga aggcaaagtt aatcaagaag tagatggttt
    12421 tttccagaaa gctataaaaa gaattgcaaa agatgaggat ttaagaaaag gtgtttttaa
    12481 agattataat gacaatttaa ttaaaaactg ttgtgaacaa ataatatatg atgaaagtac
    12541 ttttttgaaa aattctaaag aaattgcatc ttacttgttt gatgttatga aaattaatgc
    12601 tgcactagaa tcttgtgatt tggcaatttg cttatacact attaaagatg aaaagagtgt
    12661 agcaatactg aaattagatt ataaaaagtt gtacactcat tcaattgaat ttgtggatga
    12721 taaatttaat attcaaatgg tttcaaatga aattggtatc ccagagactt taagacaaaa
    12781 gcaaggagct ttgattagtc taagtggtat aaatgatgag tttcatctaa ggcttttaga
    12841 taaggatgct gaaaaagaag ggtcagaatc taagtttgta acagaatttt taaatgctaa
    12901 aaaaatagat gatgataagt acaagaccaa ggtatttaaa aatacagctg aaaattggat
    12961 aaccaatgct cttagtaatg atataaagca agctgaagat gtaagaagta tattaaatta
    13021 tacactgaag gaaaaacatg aagttgatat aaatgacttt gttgataatt caattaaaga
    13081 tgatgagtta aaggatagct ttaaagagca tatggaagaa aaaggtcttg acgaaagttt
    13141 tagtatagat aaaaaatggg ttgagaaaaa gcttaaaaaa agaagtataa aaacggataa
    13201 tggttttgac ataaaaggga atctaactga ttttgaagac ccaatgaagt atactgtaaa
    13261 acaaaatcaa gatggaacta tagatataat aataaagaat gttacgtttt atgaagaaaa
    13321 gtaatttata tacttagtat ataaattaaa tataagagcg tgtattttat atattgtaaa
    13381 taaatgtaat ctagtctgtg cgaactctaa tataatttat tatgtagaag tttacacaga
    13441 ctatttatat ttgtagacaa aacgatacta tttgttttct tcgatatatt tacatatatc
    13501 accaatacat ttaaagtttt cagcttcctc gtctggtatt tctattccaa attcatcttc
    13561 aagggccatt ataatttcaa cagcatctaa tgaatcagct tctaagtcgt ccattaaaga
    13621 tgcttccata gttatctcat ctaaattgtc tacccctaat tgttctgcta ttatttcttt
    13681 tattttctca aacattttaa gcctcctaaa gcagttttaa ttaattttaa tgttagttta
    13741 atataatatc aagaattttt caatatggaa tctataaaaa ataataaaat tttataaatg
    13801 tagtaataaa gtattatttg atgaataaac tttattgata acaaatataa cttaggagga
    13861 aatgctatga aatatgtagg actattacta acttcagtag gaatgttttt acttatagca
    13921 gtaaattttt attataattc aataacttta gatatgcaac gtatagagga ttatgtaatg
    13981 gagaccaatt taatccttga agatgtagct gaaaaggaga gttatgtatc taatgaaaaa
    14041 gaagattaca tatcaaggtt aatgcatgta aagaaaggaa tagaaaattc aaaaacatcc
    14101 ttcttgatag aaagatataa ggagtacaaa ataaaatcaa tagaaagttt aatctatact
    14161 atatcagagg agaaaaaaga ttatctggat gaagttgata gatacaataa acttggtgaa
    14221 aaagaaataa ataaactaat caataaaaac tttttagaag taacttatct gtctattact
    14281 acatatattt aattaaagag acaaaactta gtctcttaat gattcaagga ggattagtta
    14341 tgacaaaatt agaagacaat atggaacgtt tctttggagg atgcgaagga ttttttggga
    14401 ataaaatgtt gataataata gttatagtat tcttactatt atgcacagac attttagaag
    14461 acttattatg cgatgacaat atatggatat gggttatatt aatagtattg ttattattta
    14521 acttcgatga tggttgttgt tgctaaaaaa tattgattat taaataggta aaaccagtaa
    14581 cttatacaag ttatggtttt acctttttta gttcaaatta aaaaatttaa aaaaatagta
    14641 aaaatacatt agcaaaatgg aaaccaaatg catagtatat gttaggagcg aaaagctcaa
    14701 caaaatatca acaggaggta acgtataatg ttagatagat tctttggaga agaaggatgt
    14761 tttggtggag gaggatggtg gataatagta ctattcttcc tattcttagc ttttggagat
    14821 agctgggcag aatttgacat aatggcatgg ataccattcc taatagtttt attaatactt
    14881 tgctgctgtg gtggatgctt agaataagaa cacagttatt aaaaaaagga gggctttgtt
    14941 atgttagata gattttttgg agaagaagga tgctttggtg gaggaggatg gtggataata
    15001 gtactattct tcctattctt agcttttgaa gattgctgga gagacattga cataatgtca
    15061 tggatacctt tcttaatatt attgctaata acttgttcat gtggtggatt ctttgatggt
    15121 gaagtaggtt gcggctgcta atacttaaat cattagaata aaaaaatgct ggtattttat
    15181 accagcattt ttttatttta ttttttaata tgatttaaat taagaatgaa tattattaaa
    15241 tttgttgaat atagggtata atataaaata taatgtattt aaatgtactt gcttttatgg
    15301 taaaataaaa acttatataa atgtagttaa aataggaacc ttcaaaaatt tggttcagtt
    15361 ttagataaat ttagtaaaat aaggaggatg aagatgaaag gcaatataac gataaaagat
    15421 gttgctaaac aagcaggagt gtcaatatct actgtatcta gagttataaa tgattcaaaa
    15481 cctgtaactg atgaagtcaa acaaaaagtt ttagaggtta taaaagagac tggatatata
    15541 ccaaatccac ttgctagaag cttagtaaca aagaagagtc aattaatagg ggtaatagtt
    15601 ccagaagttt cagattcttt tgttaatgag gtgttaaatg ggatagaaga ggttgctaaa
    15661 atgtatgact atgatattct tttagcgaat acatactctg ataaggaaca agaacttaag
    15721 agtataaatc tattgagagc aaaacaagtg gaaggtatag ttatgatttc atggatagtt
    15781 gaacaagaac atatcaacta tatacaaaat tgtggaatac cagcgacata tataagtaaa
    15841 actgctagaa attatgatat atatacagta agtactagca acgaagaagc tacttttgat
    15901 atgacagagc atcttataaa gaaaggtcat gaaaagatag cttttataat gactagtaaa
    15961 gatgatactg ttttagaaat ggaaagactt gctggttatg agaaagcact ttcaaataac
    16021 aatatagaat tagacaagag tttgattaag tatggtggaa ctgattatga gagtggatac
    16081 aatagtatga aagaactatt agatgatgga ataatacctc atgcggcttt tgtaacaggt
    16141 gatgaggctg ccataggtgc tataaatgct atatgtgatg ctggatataa ggttccagaa
    16201 gacatatcag ttgcaggatt taatgatgtt aagatagcta gaatgtatag acctaaactt
    16261 actacagtat atcaacctct atacgatatg ggagcagtag caataagaat ggttataaaa
    16321 ttaataaata aggaattaat tgaaaataag aaaatagagt taccttatag aattgttgat
    16381 agagaaagtg ttacagaaag aaaaaaataa aactattaaa atcaatcagg tagtgcctta
    16441 ggcactacct gattttttat gtcaataata tcaaatttaa taatagatta actaaaaata
    16501 attatctaat tgcttaaata gtttattttt atctattcat gaggattaga ctttttagtt
    16561 ttcttaaata aacttttaga ttttttttgt tgactacctg gtgtattttt tggtgcaggt
    16621 gaatttgact tacctttagt aggtttagcc ttcttagttt tagctggttt agtatctctt
    16681 tggttttctg catctgagat tttttccatt atattatcca gttttttgcg ttgattttca
    16741 tctaaaaatg ctttgaattt tgatactact tcatctctgc catctagatt cttacctatt
    16801 tttataagtt cctccataag ttcattttct gattttcctt tgtaactatc agccatcttt
    16861 tctattttat ccttgtcaat atttttttgt ttagccattt tttctaatga ctctgtatct
    16921 aattttttca taaaaaaacc cctttctata aatatatatt gaattatatt catactattt
    16981 atatatagaa ataatattga agtggtgaat tgtgagtgat atataatata aataaaaatt
    17041 cttatagata atatatatgc actgagcttt aaaaatggaa cattaaaata tgaaaaacat
    17101 actttaatat agggcaatta tataatataa gggggaattg tcttgaataa cttactattt
    17161 ttgattggag tgattgcatt gagtaatggt aatatctcta tatttgatga taaaaataaa
    17221 tcaaaaggaa ataatagagt tagaaaaaac aaaaaaaatg caaatgataa actttcacag
    17281 tctacacatg gcaatttaag gtttgatgca aatgatataa aaagaggttt aaggctcatg
    17341 gatttaagtg aagaagattt agaaatggga atggaaataa taacaagaac taaaaaatac
    17401 atgtcaagag atgaaagaaa aatactaata aaaatagaaa gtgttttaga tttagttaga
    17461 ggaataaaaa aattaaataa tatagatatg gtagatacag aagatgaaac ggattttttc
    17521 agaaaaatgg atagtgatga taaaaagaat atgatgataa aggaaattat agatgtattc
    17581 ccagaaaaaa gaaaaaattc agttgaaaaa gctataggaa tgaaaaagaa gatagattta
    17641 tttgcagaat tatttttacc agatgacttt ggtgaaggtg gatttagttt gagctctttg
    17701 gcaaatataa acaatcttgg tagtatgaat aatctcaaat tgttaggaag tcttttaaga
    17761 ggtgatgata gtaataatga tgatgaagac aataatgatg agttaaatta tgaagacgaa
    17821 gaattagatt gtgaagacaa agaattggat gatgatgagt taacagatga aggtgaaatg
    17881 gaggataatg aagatgaatt agtagaagat aagaacacag gctatgaaaa tatagaaaat
    17941 gatgaagata tagtatacga tgaggaacta tacaacatta atgagcaaga atccgaatac
    18001 aattataatg aaaaaaatac taaaactaga gctaagaaaa aacgataatt aactaaatac
    18061 ataaagaaaa aaggaagaaa aagtaatgat agaataagat tatctcaatt actatataaa
    18121 accactttaa aaaattcaaa atttattata taataataat atgaaatcct gaaacatacg
    18181 ataaggcttt tttaaattca acctacaaat gaaatacggg agaccgtgtc tggtaaatta
    18241 taacatgcct agttagtttg gacgttaaag gttactggca agtcacccac ctgggagtgc
    18301 ctagggtgtg aatttactta gccaccggtg tttcgggaca aaaaaagtgc tactatttca
    18361 aatagtagta ctttttttat taatatgcat ttttctacta aatacgccta attattgata
    18421 aattcaaaaa gcaagtaaca attaagatta taaaagcata gtattaatta ggcttcaaaa
    18481 agccaaagac ttaataagaa tcgcttacag ggagggcaat gaaaaatgca agattataaa
    18541 aaaaataaaa gaagaatgat gaatcagcca atgtctacaa tgaatgaaga agaagtgtat
    18601 acagatgaaa taaattcaga agacatgaga ggttttaaaa aatcacacca tcataatgga
    18661 tgtaatactg ataataagtg tgagtgccat gatgattgca atccatgcaa cccatgtaat
    18721 ccatgtaaac ctaacccatg caatccatgc aaacctaatc catgtgatga caattgtgga
    18781 tgccatgaca attgtaaatg tgattgtgaa ccatgtgaaa tggattcaga tgaatgtttt
    18841 gaaaacaaat gtggaccaga atgctgtaat cctatatctc caagaaactt ctctgtatca
    18901 aatgcagtgc catttgcaat agaggctaat agaatatttg atactatgca attccaaaca
    18961 tttacagatg caacaggacc aaatggagag ccattaactt ttgaaacaga agtagtagaa
    19021 gtatttggtt cagttccaag tgcaggtcaa gcaagtgtaa ctatagaaaa aatatgctta
    19081 agtaatgatg gaatcgttat agacacagga atgacaactt tagaagattt cgatttagac
    19141 ccattaggag atatagtagg aagaaactgt gaaacaactt ttgaatttgc agtttgtgga
    19201 gaaagaaact ctgagtgctg tagacaagga aaaggcaaat cagtagctta taaacaaaga
    19261 ggattaactg tagcagttcg taatttagta ctagagctaa gaggtagatg tggatgtaca
    19321 gagttcgttg cattagcttt cccagcagtt agagcaggag gtggatgtaa gagaagagtt
    19381 gattatgtag aatttacttt taacacactt tcagcaccaa tatgcttgcc agctgacgga
    19441 agagctgtta ctttaagaca agaatatcaa actaacttaa ctgtagattg tataggaaaa
    19501 tctatattaa aattagaatg caacgaatgt tgtgaacctt tctatgaatt aattatacca
    19561 aatgatatag atttagtact ttgcttacaa gaaacagtta gcacattaat aagtgaacaa
    19621 atagtagttt tagcatcacc aaatccaatc caaccaagac ttgttgatac tttctctaaa
    19681 gtatgtgatt tttcgcaatg tggacctaat catggaagtg gaaagccaag ttgccacaga
    19741 tagataggta gatatataga cagtttaaat tataaataaa aatcatctaa ggaaggctac
    19801 caaagtgtat aatttacatt ggtagccttc acgtttttat aactagcgta tagattttag
    19861 tgatttaaaa agcactatag tatgtaatag acaagtaaag attgatacta tcgtcattgt
    19921 gtatatgtat gcataaatat taagtcctgg aattggaaga aatacatata aagctattag
    19981 ttgaattaac atgccagtaa tagtatttat acttgaagct aattcttttc ttattgaatg
    20041 taaaatgcca gatagagttt gatttaggga cataaatagt ggaactaaaa acatagcttt
    20101 tagatattct cctgcaagtg tatttttaaa tactaatata cataaatctt ttccaaagaa
    20161 ataaaaaaat attgatgata gtatgcctac aaataaagta agaagtatgg agtatttgat
    20221 ttttttaatt acactcttac gctttcttag agccatttct tgtgaaatag ttggtattaa
    20281 atttacaata agcgctgaac caacagtaaa aggtaaaaat ataaaaggca ttaccatccc
    20341 actgacaata ccatacatac ttaaagattg ttgataagtc attcctgata atgctaatct
    20401 tgatggtatc atcatagaac ttatagattg caaaagtatg tttgacattc tattacaagt
    20461 tatgggaata gacattttta atgtttccat agaagaatta taaaaatctt ttatgcttat
    20521 aatataccta ttgtcaaatg atgaatctcg ccataagcaa ataagcataa atataatatt
    20581 actcaactca ccaatcgata tacctaatag agctatataa caattcatag atttattatt
    20641 tatgtacata acaagtaaaa aaacaaataa tattttacca atttgttcaa tgatttgtcc
    20701 aatagcaggg atttttacat tctttatgcc atagtaatat cctcttagta cgtttgaaag
    20761 agttattata actatagcag gacaaacagc tagtataaat agatttaatt tagcatcttt
    20821 taaaaattta agtgataaaa aagaactatt gaaagatgca actatagata taattagtgc
    20881 tacgaaaaat gatatatata aagttgatat aaataaagca ttagtattgt gtctatcgtt
    20941 taaagctttt ctctttgcaa ctagacaact aagtgcagta ggtatacctg ttgttgtaac
    21001 agctaagcaa atcatcaaaa agttaaaaat catatgatag atacctaaac ctgtttcacc
    21061 tagtgctctt gataagaata ttttatataa aaaaccaaat attcttacaa taaaattgga
    21121 tataaataaa ataactgttg atagtactaa ataaggtact ttcaaaaaaa tcacctcctt
    21181 atcttttaaa tatattcaca aggaggtgta aaaatttata attttataaa ttctggtttc
    21241 aatttattaa atttagatac atatttaaat cccatatttt ttagtaatga atatatgtag
    21301 tcaaactcac atgcaacttg acttatatga tgtgaatctg aacctgtggt tatgatttcc
    21361 cctcccaagt ctttgtacat ttgaagtata tacttagatg gggttaggtc tggaagatta
    21421 tatctataac aagaggtatt tatctcgata cctttgccat cataaatagc ttgtttaaga
    21481 gtttcttcaa ttatatcaga aaatagtcta tcatctaaaa tagtgtcata aggtgcatac
    21541 cttttaatca aatctaaatg acctaagaca gagtaatctt tataattctt aacaatatta
    21601 tataaataaa gatagtaatt ttcataaact tcatgttgag ttttatcttt aaaataatta
    21661 ccaaggtata agtccattgt atctatagca tggatagaac atattataaa gtccaatgga
    21721 tattgatgtg cttccttttt gtatgtatcc attaattgag tttgaactcc aaattcaatt
    21781 ccttttttta ttgatatttt atctttatat ttgctttgaa gagtctctaa actcttaaaa
    21841 taatcttcgt agacaattga aaaagaatca tcagcatata catcatagtc aacatggtct
    21901 gtgaaacaaa tttcctccaa accgagttca atagattttt ttaccatttc ttccattgta
    21961 gattttccgt ctgtagagaa acttgagtgc atgtgatagt cgtaaaacat aaatatttcc
    22021 cctttttata aaaattttgg tattatatat acatactaaa taatatcaaa tatacataaa
    22081 agcatcaata aaatataaat acaatgagcg gtgataaatt tggaaaaata cagaatagat
    22141 ttaaatgaga aaaaatcaac taaatatata cagatattta atcatataaa agaattgata
    22201 ataaataaag agttagtaga acatgaaaaa ttgcctccaa taagaaaact atcaaatttt
    22261 ttacaagtaa ataacactac tatagttaag gtatacgagc ttttagaaaa tgaaggatat
    22321 gtatataaaa ttatagggag tggtactttt gtatcaagtt taaattctta taaaagcggt
    22381 atagataaca ggaaaaataa gattattgtg caaagagaag atttaattca ttttgacaat
    22441 gggaatccat ctaatgatat gtttcctatc gattcattta aaaatgctgt aaatatggca
    22501 ttatctaaag atggtagttc aatatttgaa tatgatgaag gtcttggctc tgaagaatta
    22561 agagaaaaaa tggtagagta tctaagtgat ggaaatataa aaactactaa ggaaaatatt
    22621 caaataatat caggtgctca acaaggtata gatatagttt gtaaaggact tataagttat
    22681 tcagatgttg tttttatgga agaaccttct tacaatggag caattgaagt gtttaaaagt
    22741 agaggtgcaa aaataatatc tatacctatg ttagatgatg gaattgatat aggtatttta
    22801 aaattaaagt tggaaaaaat aaaaccaaga cttatataca ttatgcctaa ttttcaaaat
    22861 ccaactggaa tatcttactc aacatataaa aaaaagaaat tgatagaatt ggcagaagaa
    22921 catgattttt atataataga agatgatttt ataagtgatt ttgtttttga atcaaaggac
    22981 aatagaacaa ttagaagtta tgatgataaa aatagagtag tatatataaa aagcttttca
    23041 aaaatactta tgccaggtct taggatagga attgtagaaa tgccaaatga gttgttaaaa
    23101 aaagttttat gggctaaata ttcatcagat atatcaacac caggtttaat tcaaaaatca
    23161 atgtattact atatgaataa ttttgattgg aagaattatt tgagtcatat agagaagata
    23221 tatactgata agtataaatt ggcaaaaaac ttgattaatg acaaattgag tggtaaatta
    23281 aaagttagaa aatcttgtgg aggaataaac ttttttttag aattaccaag aggatataca
    23341 tctcaagcat ttacaaattt tatgttaaac aaaggagtgt caatgcttcc agggacttat
    23401 ttttttgaca atctagtgga tgatagattc tttaggataa atatagctcg tccaagcatg
    23461 gaagaactag aaaagggaat atctataata agtgataata tagatgagtt tttttgtgaa
    23521 tataaaaaca agttagatat aaaaagcaat aaattatttt attaaataaa tagaggtttt
    23581 ttaggaggaa aaaatgtttg acaagaagaa attagatagg attaatgaat tagcaaagaa
    23641 aaataaagag ggaatccttt ctgctgatga aatcaaagaa agagaaattt taaggaaaga
    23701 atatttagaa aactttagag cccactttag atcaagacta gatagtgtca aagtagtttc
    23761 accagaagaa tatgagcaat acatgaaaaa caataaaaat tagaacttag taggaggaaa
    23821 atatgaactt aaagtacgaa tttaagaatg cttgggattt tgaaagaaaa gaaaatacta
    23881 ttgaacaaat tatgcaatat tctagcaatt atatggagtt tttaagtaag tcaaagactg
    23941 aaagactttc agtaaaagaa attataaaat tggctaaaga aaataactat atttcaatag
    24001 atgaagctat ggaaaaagga agcataagtt gtggtgataa aatttatgtt ataaataaag
    24061 agaaggcagt agcacttttt gtaataggta aaaattatat agaaaaaggt atgaagatta
    24121 taggaagcca tatagattcg ccaagattag atttaaaacc aaatccactg tatcaagaga
    24181 gtaatctagg tttttttaag actcattatt atggtggaat aaaaaaatac caatggacgg
    24241 ctattccact tgctttacat ggtatagtta tattaaatga tggaacaaaa gtggacatat
    24301 ctattgggga agaagatagt gacccagtat tttgtgtaac tgatttattg attcatttgg
    24361 ctggagacca aatgcaaaag aaactatcag aaggaattag tggagaagct ttaaatatat
    24421 taattgggaa tatgccttta gaagatgaag aaaaagaacc tataacagca aatatattaa
    24481 agattttaaa tgagaaatac aatatagttg aagaagattt attaagtgct gaaattgaag
    24541 tagttccagc tggtaaggct agagatttag ggcttgatag gtctatggtg ttgggatatg
    24601 ggcatgatga tagggtatgt tcatatgctg ctgtaaaagc tatactagaa actgaacaac
    24661 cagaatttac ttcagttgca ttatgtgtag ataaagaaga aattggttct aaagggaata
    24721 caggtatgca ctcaaagttt tttgaaaata ctgtggctga actgattgct ttagaaggag
    24781 attattgtga tattaaagta agaagagctt tagcaaatag taaggtatta tcagctgatg
    24841 tttctgctgg atatgaccca aattttggag aagcatatga aaagagaaat tcggcatata
    24901 tgggaaatgg agtagtttta actaagtata caggttcaag aggaaagagt ggatgtaatg
    24961 atgctaatgc tgaatttatg tcagaagtaa agagaatttt taataaagga aatgtagtat
    25021 ggcaaactgc tgaacttgga aaagtagacc aaggaggagg cggaactata gcccatatat
    25081 tggcaaatca aggtgcagag gttatagact gtggtgttgg agttttaaat atgcatgcac
    25141 cacatgagat tgtctctaaa gtagacatat atgaaatgta taagggatat aaggcatttt
    25201 ttaatataaa tttataagta aaaatataaa tgtaataagt gaataaaggc tgttgtataa
    25261 acaatttaat ctgttgtaca acagtcttat ttttatgtaa aaatagcaat ttgtcacact
    25321 agagaaaata aaagaaaata ctgtcaaatt aaaaattatt ttaaaaaaaa ttgacaacga
    25381 tttcatcttg ttataatatg taataaagaa cttgttatat aatgttataa aaatgttaca
    25441 tagggaggtg agcaaaatgg atgaaaagag acaaaaaaaa gaaatggatt tagaagtaaa
    25501 ttcctttgtt cctttatatc aacaactata tgacaacata aaaaaacaaa tagcatctgg
    25561 catatataaa ccaggagata aacttccatc tgaaggagac ttatgtaaag aatttaatat
    25621 aagtcgtata actgtgagaa atgcacttaa tgaacttgta aaagaagata tattatgtaa
    25681 aaaacgtggt aaagggactt atgtaacaat accagagaga atagaagcaa catgtgcagg
    25741 aaatagtttt actaattctt gtcatcgtat taatgcaaaa ccaagtacca aaataatctc
    25801 tgttttaata aaaaaagcag ataagcaagt agctgaagct ttaagtattg aactagaaga
    25861 gaaggtaata tgtattaaaa gacttagatt aatagatgaa gtacctgtta tttttgaagt
    25921 tgactatttt agaatagatt acatgttttt attaaaagaa gaattagaag ataaatcttt
    25981 aatggaggtt atatctaata atataagcac attacctaaa cgagtagaaa atatatttga
    26041 agtaaaacat tcaaacaaag aatattctga ccatcttaaa tgtgcaagta atatgccact
    26101 attgaaagta agacaatcgg tgtatacaga aaataatgat gtcttatatt acaatgagca
    26161 atttataaga agtgataagt acaaatatgc agtatcagca gaaatataat ctataattta
    26221 gtaggaggat gagtatgaaa aagataatat tagcatctca tggagacttt tcaaaagggc
    26281 ttttaaatgc agtaaaaatg atagtaggag atttagcaga ttgtgtcagt tcgtatggtt
    26341 tgtaccctgg acaaagtgct tcagattttg ctttagaatt agaaaaaaca atatcagaag
    26401 ataaagaatg tgaatatata attttatctg acttatatgg agcaagtgta tgtacagcaa
    26461 tgttaagatt aactaatcta cataatgtta ggttattttc tggaatgaat ttgaatatgg
    26521 tattagaact tcttacaagg ttttctgatg aattaaccgt tgaagatata gaccaattag
    26581 tggaagaatc tagaagagga atacaaagcg tgacattaca aattaaagaa gaagaagagg
    26641 tattttagtt tatgaaaaaa aagggaatat tatcagtatt taaaagtaaa aaaccaatta
    26701 tagcaatgat acacttaaaa ggagatactc cagaagatat ctttgaaaga gctaaaaaag
    26761 agattacaat ctttgaagaa aatggtgtag atggaataat gttagaaaat tactatggta
    26821 attattatga tttagaaaga atacttgaat atgtatcaaa agctaattta tcaataccat
    26881 atggagtaaa ctgtttaaat gttgatacta tgggatttga actggcaaca aaatataatg
    26941 ctagttatat acaggttgat tctgtagtag gtcatgttaa acctagagat gaagctactt
    27001 tagaagagtt ttttaaatta caacgttcaa aatgcccagc gtatttaatt ggaggcgtac
    27061 gttttaaata tcagccagta ttatcagaaa atgatgttga agaagattta aaaataggaa
    27121 tgacaagatg tgatgctata gcagttacag aaaatgctac aggtcaagaa acatcaatgg
    27181 aaaaaataga actgtttcgt aaaaatttag gagacttccc attggttata gctgctggag
    27241 ttacattaga aaatgcaaaa aaacagctta aattaggaga tatggcaata ataggaagtt
    27301 actttaaaga taattacaaa gattttgggg atgttagtgt agaacacgta aaaacattca
    27361 tggatgaaat caaaaaaatt agggaggaat tataatgatt aaattagtaa gagtagacca
    27421 cagacttata catggacaag tagcatttac atggacaaag tttttaagca cagactgtat
    27481 attgatagca agtgatgact tattaaaaga tgaattgaga atggcaggac ttaaaatggc
    27541 taaaccatct aatgttaagt tagtaatgaa aagcatagca gattctataa aagcacttaa
    27601 ctcaggtgtt actgataaat acaacttatt gatactttgt gaatctgtag aagatgttta
    27661 tagacttgct aaagaggtaa aagctattaa atctataaac cttggtggaa caaaatcaga
    27721 tgataatcgt gaaaatatat ctaaagcagt gcatgtatca aaagatgata taaagatgat
    27781 taaagagtta gattcagaag gcgtaaatgt atttgtacaa ttagttcctg atgatgatgc
    27841 tacaaatgta atgaaattaa tataatttta gggggatgaa aatggaattt acacaagtta
    27901 ttttgataac tctaattgca ttctttgcat atatgcatag ttttgtaggt tctacaatgc
    27961 ataatagacc tatagttgtt gcaccacttg ttggtttagc actaggtaat cttcatacag
    28021 gtatagtaat aggttcaaca ttagaattag tatttatggg tgcatttcct gttggggcaa
    28081 gtaatcctcc tgattttgtg tctggttcta ttattgccac agcgtttgta atattaacgg
    28141 gtcaagatgt atcagcagca gttgtacttg cagttcctat agcaactcta gtattgctta
    28201 tagataactt cttaatgaca gtagtactta catggggagc tcatattgct gaccgttatg
    28261 cagaagaggg gaatattgaa ggcgtagaac gtgtgcaatt attatttggt ataggaaata
    28321 aattaatatt agcaataata gtaggtatag gattttcatt aggtgttcca gtaatagaaa
    28381 aaatactttc atttatacca tcatatgtaa ctcatggaat ggatgttgca gcaggcgtga
    28441 ttccagcaat aggttttgcc atgttagcta gaatgatgct taataagaaa acagtagcat
    28501 ttttattatt aggatttata ttggttgctt acctaaacat aacagtaaca ggagttgcat
    28561 tgtttggatt agcaatagct ttaatatatg ttaactttgt aggagagaag gaggtaatag
    28621 tagatgacaa cgagttctaa gaagttagag acaatttcac cagatagtaa aataacgagg
    28681 aaagactttt ggaaatgttt tagaaggtca ttgacattag attcatcttg gaactacgaa
    28741 cgtatgcaaa atatagcata tgcatacatg atggcaccaa taatccgtag attatataaa
    28801 gatgataaag aaaagaaatc taaggcttta aaaagacatt tagaatttat gtcagttact
    28861 cctcatataa gtactctttt ggtaggtata tctggagcta tggaagaaga aaatgcaaag
    28921 aataaagaat ttgatgctaa tagtataaat gctgttaaat caagtttaat gggtccagtt
    28981 tctgggatag gagattcatt tttctgggga acattaaagc ttatagcagc tggggttggt
    29041 atagctttag cttctcaggg taacataatg ggaccgattt tattcctttt aataataaat
    29101 gttcctcatt ttattatacg ctatatatgt ctagataaag gatttaagta tggaactcaa
    29161 ttctttaaag atgtgagtgg ttcaagtata gtttcaaaag ttatggaagc agcttcaatg
    29221 ttagggctta tggtaattgg aggtatgact gcatctaatg taatgttaaa acttagtgtt
    29281 aatgtaggta gtggagaatg ggcagagcct attcaaactt acttagacca aataatgcca
    29341 tgtatgcttc cagctatgat attcggtatt atgtattggt tattaggaaa aaaggtaaag
    29401 acaactacga tattaatttc agttatgatt atttgtatag tattggctgc tattggagta
    29461 gtataaataa aaaaatccca agttcatttg aacgagggat tttttgacta aattaaataa
    29521 aaaagaaaga tggttcataa taatttataa gcatttatgt gatagttata attagcttat
    29581 taattagtta taaactcaaa attaagcact actttattaa agttattata ttataatttc
    29641 gaaatactca cataatctgt ttagaaattt atcttctaat ggggaaaaag ttttcttttt
    29701 agagtaaatg aagaaaaact ttctagtaaa atcaacatcc tttattttat aacattttat
    29761 tttacctgca ttgagataat cgatagctga aatgtacgat ataaaggaca ctccaagacc
    29821 taatctaacc atttccttaa tagcctcatt tgattctaca tgagcgatta catttaattt
    29881 acttactgga aagttatttt tactaagtgt gtttagtatt aagtttcttg tacctgaccc
    29941 ttcttttctc ataataaaat ttagataagc caattctcct atatcgatac aattattttt
    30001 attatcaatt ttaaaatctg aaggagtgat aagaacaagt tcatcatcta ataagtctag
    30061 gtactctatt tgaggattat tgattttaga accaactaat ccaaagctta ttcgttcatt
    30121 taaaatttct gatatagcat attgagaatc ataatggctt atggaaaatt ttacatctgg
    30181 ataactcata gaaaaactct tcataaaatc aggtaaaata taagtttcag gtatggaact
    30241 acatgctata tctataatac cttctatttt tcctgaatat tcttttatat cataaattgc
    30301 acgtttgcaa ttattcaata tatatatagc atgttcataa agaatttcac cagattttgt
    30361 gagtgtaata actttgttgc ttctatttat aagaacagtt tctagctctc tctctaaatt
    30421 ctgtatgtgt gcgcttacag taggttgagt taaaaaaagt tctcttgcag cctttgaaaa
    30481 actctgatgt tttgcaacag caacaaatac ttcaagttgt ttaaaatcca ttatttaatc
    30541 ctccttaaat tatctaaaac tattataaca tatttgataa attaatagtc aaaggtgatt
    30601 tttaaaatag aaaaccttta aatattcatg gagtttttga ttgtttagga ataatttggt
    30661 ataatgttgt aaggtactta caataaaaat atcgtaaatt gaattttcaa aattcaactt
    30721 taaagtgttt tttattacag aaaaattaca attagcttct taatcttatt aaattcaaat
    30781 aaatattata taattatttt ataatataat aaatataatt attgaaaaaa gagataactg
    30841 taaattgaga tatagataaa tactataaaa tttaagaaag gaggattgat ttatgaagtt
    30901 aaaaaggtca ttagtttgtg taactatttt aggtattata cttgttggat gtcataaaga
    30961 aaatactaaa gagaaaaatc aagtagcaag taaagcaaca cagcaaaaaa ctatgacaaa
    31021 agttcaaaat gatgttaatg aaattatgaa taaagattat aagtacatta taaagaatat
    31081 gggtatacct tataatacat tttattatat aaaacctaaa gttttaaaag aatcaaatac
    31141 aatgcaagat ataaatacat caagttatat gacattagtt tatccaaaat atactggaaa
    31201 tgatgaacta gatggaagtg ctttatatgt tgatattaac gaaaataaag ttgtcaatgt
    31261 tgaaaccaat tctttctcat cacaaggtat atcagtcatt gatgctgaat ctagtatagt
    31321 gattgaaaaa agtgaccatg aaaaatcggc tgtatcacta gaaaatttta gacatattga
    31381 cctgggagag tatgttggtg ttgaagattc cagaataaat gaaattgtag gagatgcgaa
    31441 ttatgattta acagcatata atcatgaagg cagtaaagta gttaagagtt atagattgaa
    31501 agaagataac aaaatattaa aaaaagaggt acttacaata agtattgtgg ataacaaaat
    31561 aaaatctata aaaactattg aaagtgataa aattgtaaaa ataataaaag gaactttatt
    31621 agaataaaat attctaaagc atatgatgct gtttcaagat agaaggaaat ttctagctgg
    31681 gaacagtttt tttattagat ttataaaatt tgaactgcac ttatagataa ctattagtta
    31741 taattaaata taagataaaa gtatttccaa cttttataat ctataatata ttacatagaa
    31801 atatcttaaa taaaaaatat attggggagt atttaaaatg atagaattag aagatgccat
    31861 aaaaattatt gaaaaaaatg ttttaccaat aaacagggtc aagatagtaa atcttataga
    31921 tgcagtaaat aaggtagttg cagaagatat atattcaaca attgatagtc ctccatttga
    31981 taggtcaccc tatgatggtt atgcatataa tgcaaattct gaaaatagaa atttaaaggt
    32041 tatagggact ttgtatgcag gagatgtatt taatagggta ttaaataaaa atgaagcagt
    32101 gaagataatg actggtggaa agataccaca gggtgcaaat tgtgtaataa aaaaagaaga
    32161 tgtaacagtt ataggtgata tgataacttt aaatataaaa ttaaaggaat acgataatta
    32221 catatttaag ggtgaggata taaaaaaggg gcaattgatt attaataaaa atcagaatat
    32281 aggatatgat ggtattggag tattagcaag tcttggaata tccaaagtaa ctgtttatga
    32341 tgatttaaaa gttggaattt tgaatacagg tacagaacta caagatataa atgagacttt
    32401 agagaatggt aaaatatacg atagtaatag atatacctta tattcaagat tattaaaact
    32461 tggaatacaa gcagatacaa tatgcaattg ttctgatgat atattggagt tagaaaaaaa
    32521 ggtaaagaat atgttacaga gtgtagattt tttaattacc acaggtggtg tttctgttgg
    32581 agataaagat ttaattccag atgtattcaa aaaaataggg gcaacagaat tattttggaa
    32641 gataaacatt aaacctggtg gagcttgtta tgtaggtaca ttgggagaaa agctattatt
    32701 tggtctttct ggtaatccac atgcagcaat agtggtattt gataatgtga tagttcctgt
    32761 tattgagtat ttaacatata agaagggaac acatcatata aaagctatat ttaaaggaga
    32821 atttaataaa ttttcaaata aagatagatt tgtaagtgga aaactatata caagtgaagg
    32881 taaattgtat gtagagctaa atgataagaa agatgcaaaa gctagactat ctgcaactct
    32941 aagaagtaat tgtatgatta agattaaaaa gaatgagaca ataaaagaaa atcaattagt
    33001 ggatgttgtt attagatagt tttatgaaag gacacaattt tatgtataat atattgtcag
    33061 ttgtctcata ttcaggagta ggtaaaacta cgttgattga aggcataata aaagaattaa
    33121 ataaaaaagg gtatcgtgtt ggagttatta aacatacctg tcatgatttt gatatggatg
    33181 aggaaggaaa agatacttat aaacatagaa aatctggggc gagaaaagtc tgtataatat
    33241 cagataaacg agtagcatat attgaagaac taaaagagaa gaatccatta tataaaatga
    33301 ttcaattata tgaagatatg gacttgatta tcatagaagg atataaaaat tatagattta
    33361 aaagactgga agtaactaga aaaggtaagt atgaggatat tttatctaat aagtctgatt
    33421 tgataggtat tgttagtgat atagaatata aattgaatat tgaacagttt aatttaaatg
    33481 attataaaaa tatatcaaag tacatagagt cttgtataaa aaatgaaatt ttaagtataa
    33541 gcaatgttga aataaaaaac atattaaaag aataatttta agatgttctt cttatataaa
    33601 atgatataat tattt
//
LOCUS       DBGJFU010000033        32603 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.033, whole genome shotgun sequence.
ACCESSION   DBGJFU010000033 DBGJFU010000000
VERSION     DBGJFU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.033"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..445
                     /locus_tag="ACOIAS_001504"
     CDS             <1..445
                     /locus_tag="ACOIAS_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGZ2701219.1"
                     /translation="LKRYNNIILYDYLYNKGEYSYIYENKREESEIIKYYNKTDLLYL
                     KNVVSNIVPSRDEFITIYKQALIKKEIDLDMVNIRETFNVIPLKFFTILNVFRELNLL
                     DFNLNYEKNSVLIRILPKPQKKLDLNESLILNNLKNLEKQYNSSY"
     gene            458..970
                     /locus_tag="ACOIAS_001505"
     CDS             458..970
                     /locus_tag="ACOIAS_001505"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGZ2701220.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            1044..3251
                     /locus_tag="ACOIAS_001506"
     CDS             1044..3251
                     /locus_tag="ACOIAS_001506"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HGZ2701221.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            3276..3725
                     /gene="dtd"
                     /locus_tag="ACOIAS_001507"
     CDS             3276..3725
                     /gene="dtd"
                     /locus_tag="ACOIAS_001507"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGZ2701222.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            3740..4354
                     /locus_tag="ACOIAS_001508"
     CDS             3740..4354
                     /locus_tag="ACOIAS_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2701223.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            4445..6001
                     /locus_tag="ACOIAS_001509"
     CDS             4445..6001
                     /locus_tag="ACOIAS_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HGZ2701224.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            5991..7253
                     /gene="hisS"
                     /locus_tag="ACOIAS_001510"
     CDS             5991..7253
                     /gene="hisS"
                     /locus_tag="ACOIAS_001510"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGZ2701225.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            7327..9114
                     /gene="aspS"
                     /locus_tag="ACOIAS_001511"
     CDS             7327..9114
                     /gene="aspS"
                     /locus_tag="ACOIAS_001511"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGZ2701226.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMLTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            9573..10805
                     /locus_tag="ACOIAS_001512"
     CDS             9573..10805
                     /locus_tag="ACOIAS_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGZ2701227.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            10819..11625
                     /locus_tag="ACOIAS_001513"
     CDS             10819..11625
                     /locus_tag="ACOIAS_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HGZ2701228.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(11737..12606)
                     /locus_tag="ACOIAS_001514"
     CDS             complement(11737..12606)
                     /locus_tag="ACOIAS_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2701229.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            12978..14405
                     /locus_tag="ACOIAS_001515"
     CDS             12978..14405
                     /locus_tag="ACOIAS_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HGZ2701230.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIGSVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(14482..14751)
                     /locus_tag="ACOIAS_001516"
     CDS             complement(14482..14751)
                     /locus_tag="ACOIAS_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701231.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(14754..16196)
                     /gene="nhaC"
                     /locus_tag="ACOIAS_001517"
     CDS             complement(14754..16196)
                     /gene="nhaC"
                     /locus_tag="ACOIAS_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HGZ2701232.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(16221..17387)
                     /locus_tag="ACOIAS_001518"
     CDS             complement(16221..17387)
                     /locus_tag="ACOIAS_001518"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2701233.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKYYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(17612..19732)
                     /locus_tag="ACOIAS_001519"
     CDS             complement(17612..19732)
                     /locus_tag="ACOIAS_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2701234.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSNNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            19965..20366
                     /locus_tag="ACOIAS_001520"
     CDS             19965..20366
                     /locus_tag="ACOIAS_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701235.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            20409..20702
                     /locus_tag="ACOIAS_001521"
     CDS             20409..20702
                     /locus_tag="ACOIAS_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HGZ2701236.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            20717..21157
                     /locus_tag="ACOIAS_001522"
     CDS             20717..21157
                     /locus_tag="ACOIAS_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HGZ2701237.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(21249..21722)
                     /locus_tag="ACOIAS_001523"
     CDS             complement(21249..21722)
                     /locus_tag="ACOIAS_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGZ2701238.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(21722..22510)
                     /locus_tag="ACOIAS_001524"
     CDS             complement(21722..22510)
                     /locus_tag="ACOIAS_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGZ2701239.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            22838..24082
                     /gene="glyA"
                     /locus_tag="ACOIAS_001525"
     CDS             22838..24082
                     /gene="glyA"
                     /locus_tag="ACOIAS_001525"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGZ2701240.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            24405..25502
                     /locus_tag="ACOIAS_001526"
     CDS             24405..25502
                     /locus_tag="ACOIAS_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2701241.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            25493..26191
                     /locus_tag="ACOIAS_001527"
     CDS             25493..26191
                     /locus_tag="ACOIAS_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2701242.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            26194..27258
                     /locus_tag="ACOIAS_001528"
     CDS             26194..27258
                     /locus_tag="ACOIAS_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HGZ2701243.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITSMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            27441..28145
                     /locus_tag="ACOIAS_001529"
     CDS             27441..28145
                     /locus_tag="ACOIAS_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2701244.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            28138..29460
                     /locus_tag="ACOIAS_001530"
     CDS             28138..29460
                     /locus_tag="ACOIAS_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HGZ2701245.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            29647..31020
                     /locus_tag="ACOIAS_001531"
     CDS             29647..31020
                     /locus_tag="ACOIAS_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701246.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEAFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(31392..32330)
                     /gene="pdaA"
                     /locus_tag="ACOIAS_001532"
     CDS             complement(31392..32330)
                     /gene="pdaA"
                     /locus_tag="ACOIAS_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HGZ2701247.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        12350 a         3887 c         5460 g        10906 t
ORIGIN      
        1 cttaaaaaga tataataata ttattcttta tgattattta tacaataaag gcgaatattc
       61 gtatatatat gagaataaaa gagaagaaag tgaaattata aaatattata ataaaacaga
      121 cctattatat ttaaagaatg tagttagtaa tatagttcca tcaagagatg aatttataac
      181 aatttataaa caagcactca taaaaaaaga aattgatttg gatatggtaa atataaggga
      241 aacttttaat gtaattccat taaagttttt tacgatatta aatgtattta gagaattaaa
      301 tttacttgac tttaatttaa attatgaaaa aaattctgta ctcataagaa tattgccaaa
      361 accacagaaa aaattagact taaatgagag tttaatactc aataatctaa aaaatttaga
      421 aaaacaatac aatagcagtt attaggaggc gtttcccatg gatttaaaaa actttataag
      481 gaatattgat gatttcccaa agccaggcat agattttaaa gatgtgacaa ctctatttaa
      541 agatggagat gcatttaaat atgcagtgga ttctatagtt gaagaattga aagataaaga
      601 tgtagattta gtaataggac cagaagcaag aggatttttg atgggtacac ctgtagcata
      661 tgctttagga gtaggatttg ttccaattag aaaacctgga aagttaccag gtgaagttga
      721 gagttatgag tatggattag agtatggtac agatacttta gagatacata aggatgcaat
      781 taaaaagggt caaaaagtag caatagtaga tgatttattg gctactggtg gaactatgga
      841 agccgctgcc aagcttgtag aaaaattagg tggagaagta gtttctatgc aatttttgat
      901 tgagctaaaa ttcttaaatg ggagagaaaa gttgtctaac tatgatgtta attctcttat
      961 aaaatactag tacttttata atagttggta taacaccaac tattttcata tatattaaaa
     1021 ttttatacaa aataggtggt tatatgcatg ataaagaatt acaagaaata attgaaaaaa
     1081 taaaagttta tgcacctaat gcaaattttg aaatggttga aaaagcctat aatcttgcaa
     1141 aaaaagcgca tgaaggacaa tatagaaaat ctggggaacc atactttatt catccattag
     1201 cagttgcaaa tattttaata gagatggaat tagatattga aactataaca gcaggtttac
     1261 ttcatgatgt tgtagaagat acagaatata cctatgaaga cataaaaaaa gaatttggca
     1321 aagaagtagc ggatttggtt gatggagtaa ctaaacttgg tcaaataaaa tatcgttcga
     1381 aagaagaaac tcaatctgaa aatttaagaa aaatgttttt ggcaatggcg aaggatataa
     1441 gggttatact tataaaactt gctgatagat tgcacaacat gagaacatta aaatacatgc
     1501 ctccagaaaa agcaaagtat aaagcaaaag agacacttga aatttatggt ggtatagctc
     1561 atagattagg aatttccaag ataaagtggg aactagaaga cagagcatta agatttatgg
     1621 acccagaagg gtattatgat ttagttagta gagtatcaat gaagagaagt caaagagagg
     1681 attatattca aggaattgtt gaacttctta aagataagtt taaagattta agtataccat
     1741 gtgatgtata tggtagacca aagcattttt acagtatata tagaaagatg caaaaaaaac
     1801 ataagacttt tgaagagatt tttgatttaa cagcagtaag gatattggtt gataatgtaa
     1861 aggattgcta tgcagtactt ggtatagttc atacattatg gagacctatt ccaggaagat
     1921 ttaaagatta tatagcaatg ccaaaaccca atatgtatca atcattacac accactgttg
     1981 taggtcctga tggagaacct ttagagatac aaataagaac tcatgagatg cataatatag
     2041 ctgaaaatgg tatagcagcc cattggaaat ataaagaagg tatctctagc aaagagtcaa
     2101 aggttgaaga aaaacttcaa tggttgagac agatgatgga gtgggaaaaa gatttaaaag
     2161 acccacaaga gtttatggat gcacttaaag aagatgtatt taatagtcaa gtttatgtat
     2221 ttacgccaaa aggagacgta attgaattac cagcagaatc aacacctata gattttgcct
     2281 atagagttca tacaaatgta ggtaataaat gtgtaggagc taaaattgat ggaagaatag
     2341 ttcctattga ttataagctt caaaatggta atattgtcga ggtacttaca tcatctaatt
     2401 ctaatggacc aagtagggat tggcttaata tagtgaagac cccaaatgca aaaagtagaa
     2461 ttagacaatt tttcaagaag gaaagaagag aagaaaatgt tgaccgtgga aatacaatat
     2521 tagaaagaga atttaagaag catgatatac cacaaaaaga ccctcttgtt gaaaaatata
     2581 tggcattagt tgctaagaaa tttaatcagc caagtgttga ggacttaatt gccactgtag
     2641 gttatggtgg tataatgtca tctcaagtgg tatctaaggt aaaagaccta tatttaaaag
     2701 aggttaaaaa agctaaaaag gaacaacaga tacaagaaga agatttaaat aaacacaata
     2761 taagtgaaac agaatacagt aaaaagagaa agaaaaacgc atcacaagga attatagtaa
     2821 aagggcttga caatatctta gtaagatttg ctaagtgttg taatcctctt ccaggagatg
     2881 aaattgtagg atatattact aagggtaggg gagtagccat acacagagct gattgcccaa
     2941 atgcaaacaa taatggtgat ttcttccaaa atagattggt ggaggtttca tggagttcag
     3001 caggaaaagg taagtttgaa gcggaaattc aaataaaagc tattgacaga agaggaattg
     3061 ttaatgaaat aactcatatt gttgcaatgg ataaacttag tttaaacgga ataaatgcaa
     3121 gaaaaggtaa ggatagtatt gtaagtataa acttacttgt agaggttaat gacacagagg
     3181 aattaaaaat tctaatgaaa aaattaaaag caattcctgg tgtagaaagt atttatagaa
     3241 tgacaaatta gagttaaaat aatagggagg aaactatgag agcagtagtg caaagagtat
     3301 cttcatctaa agttacagta gatgaaaaca ctataggtca aataaataag gggttactag
     3361 tattgttagg agtgactcat gatgataaat caagtgatgt agattatatg attgataaaa
     3421 tattgaatct gagaattttt gaagatgaaa atgacaagat gaatttatct ctaatggata
     3481 taggtggaga gcttttagta gtatctcaat ttactttata tggagattgc agaaaaggaa
     3541 gaagacctgg atttagtaat gcagctagac cagaacttgc aaataacctt tatgaagaat
     3601 ttgtaaaaaa agcaaaagat aaaggtgtga cagttggtac aggtcaattt gcagctcata
     3661 tgatggtaga gttaactaat gatggtccag taacaatact attagactca agtaaatctt
     3721 tttaaggggg aaaactttaa tgattataaa aaaatttgta gatagttatt ttggagtaaa
     3781 tacttatgta ttaggtgatg aaaagactaa aaaatgtgca gttatagacc caggtggaag
     3841 tttagtagag acattaagtt ttgtaaaaga aaatgaatta aatattgaat atattatcct
     3901 tactcatggg catggagacc atataggata tgttaaggat attaaagaaa aaactggtgc
     3961 taaagtagtt gcacatgttg atgaaaaaga attattaaat gacaaaaata aaaatctaag
     4021 ttatacaatg agatgtggag cgcaagagtt tgatgcagat atctatgtaa atgataaaga
     4081 caagcttgaa cttggtgaat taaaaatgac tttcttgcat actccaggac atactcaagg
     4141 atgtatgtgt ataagagttg aagatgaaat gttcacagga gatactttgt ttgctggaag
     4201 tattggtaga actgatttat atagtggaga ctttaaccaa atggaaaaat cacttaagaa
     4261 attatgtaaa tatgaagatg ctgttagagt atatcctgga catggaccaa ccagtactct
     4321 aggggttgaa aagaagacaa atccttacat gtaattttat attagtattt aaaaaagtat
     4381 aaaatattta ataactatat tagtgagggg gttcttctac tgttaaatag aaggagttaa
     4441 gttgatgttg tatgtttttt taaaaggaca tgattataag tatgaagtag cagaacttat
     4501 aaaattgttt actagtgaat ttaagtttaa agactctaat gctaacaata atatagagtc
     4561 aaagcattta ataaatagat tgatttatga gaatggtgta ttattctcaa gtactgaata
     4621 ctatgaaaat ggaaacttaa agtacgagtc aatacagaat atagaaaata tgaatcttga
     4681 atttttagat ggatttgata gcagcaaaac gattgaagaa tttcttaata gcctagatag
     4741 tgaaagtaag aggaacttta aaaagctgtg taaagaaaat atcaaaaaaa gcatgttttt
     4801 agtgcttaaa caagtattta attcttatgt accatgggga attttgactg gtattagacc
     4861 agtcaaaatt gtccacaatt taatggataa aaagctagat gataatagca taagaaccat
     4921 tctaaaagat aattatttta taatagatga gaaaatagat ttagctcttg aaatagctaa
     4981 aagagagcga ttatttatgt atcctataga taaaaataaa atttctcttt atgtaagtat
     5041 tccattttgt ccaactagat gtgtatattg ttcatttcct tcaaattcat taaaacaatt
     5101 tggacattta aaaagagaat atgtttataa attgattgaa gaaattaagg ggtttgccaa
     5161 agttattaag gatactaaga aagaagttga aactctatat attggtggtg gaactccaac
     5221 tacacttgat gaggaagaat tggatttgct tataaattca ttgttcaatg aattagattt
     5281 aagtaaaatt aaagaattta ctgtagaagc aggtagacct gacactatta cagaacaaaa
     5341 actaagagtg ttaaaaaaac acaatgttag tagaattagt ataaatcctc aaactatgaa
     5401 tgatgatacc ttggtaaaga ttggaagaga gcatagagta tctgatttag tagattgctt
     5461 taatatggct cgtagaatgg gatttgataa tataaatatg gatataattt taggtcttat
     5521 agatgaagac ttaaatatgg taaagaacac attggaggag ataaaaaaac tttctcctga
     5581 aagccttaca gttcatacat tagccataaa gagagcatct aacttaaata taaatatgga
     5641 taggtataaa gaacatctaa cacaatatga agaaatggta aaaatgatag atttatcaat
     5701 gaaatatgca aaagatatgg gattaaatcc atattatatg tacagacaaa aacatatgtt
     5761 gggaaattta gaaaatatag gttatgctaa agaaggatat gagtgcattt ataatataca
     5821 gattatggaa gaaaaacaaa gtaactatgc attaggtgca ggtgctatat cgaaatttgt
     5881 ctatgtagat gaagatagaa ttgaaagagt agaaaatgta aaaaatgttg aacaatacat
     5941 tgaaagagta gatgaaatga taaaaaggaa gaaagaagag gtatataaaa atgttgacta
     6001 aagctcctag aggaactaaa gacataactc caaaagaggc ttacaaatgg cgttatgtag
     6061 aaaataaatt tagagaaata tgtgctttat atggatatga agaaatggta acacctatat
     6121 ttgaacatac agaacttttc aagagaagtg ttggagatac tacagatata gttcaaaaag
     6181 aaatgtattc ttttaaagat aaaggtgata gagaaattac cctaaagcca gaaggtacag
     6241 caggtgtagt aagagcattt atagaaaata aattatatgc agatactcaa ccaacaaaat
     6301 tattttatgt gacaccttgt tttagatatg aaagaccaca agctggtaga caaagacaat
     6361 ttcaccaatt tggaatagaa gcacttggaa gtgatactcc ttcaatggat gctgaaataa
     6421 tcgcactagc tgttcaattt tttaatgaag ttggattaaa tgatttggta gtgagtataa
     6481 attcagttgg ctgtccagtt tgtagaaaag agtacaatgc tttattaaaa gagtatcttg
     6541 actcaaaagc tgatatattg tgtgatactt gcaatgagag aagagaaaaa aatccaatga
     6601 gggttataga ctgtaaaaat cctacatgta aagaaaatat caaagatata ccatttattg
     6661 cagaccattt gtgtgatgat tgtaaatctc actttgataa acttcaagaa tacttaaagg
     6721 aaatgaatat aaactttgta attgataaaa caatagttag aggcttagat tactatagaa
     6781 aaactgcttt tgaaattata tctaatgata taggagctca aagtactgta tgtggtggag
     6841 gaagatatga tggtcttgtt gaacaattag gaggaccaaa aggtataagt ggaattgggt
     6901 ttggactagg aattgaaaga cttttactta cacttgaagg taatggaatt gaaatagaaa
     6961 accctcaatc tacggatata tttatagtaa ctataggtga agaagcaaat acaagaagct
     7021 ttaaattgtt aaaagacttg agacaaaatc atataagtgc agataaagac catatagaga
     7081 gaagtgttaa agcacaattt aaatattcag ataaaataaa ttcaaaattt acaatagtaa
     7141 ttggagatga tgagcttaaa aatgacactg cgacattaaa aaatatgaaa acatcagagc
     7201 aaactactgt aaaattaagt acattagttg aagaattaaa gcaaaagctg taaaataaga
     7261 aagtatgatg ttttgtgtat tagataaata tatagcatta aaattaaata aaatggaggt
     7321 agtatcttgg aaactttaaa gggtttaaag agaactcact actgtggaga gttgagagaa
     7381 aaaaacataa atgaagaagt cgtacttatg ggatgggtac agaaaaaaag aaatttaggt
     7441 ggtcttgtat ttgttgattt aagagataca agtggattat gtcaaatagt ttttgataca
     7501 gatgtagata aagaagcttt tgaaaaagct gaaaaattag gggctgaatt tgtaatagct
     7561 gtaaaaggaa aagtatgcga aagacaatct aaaaacccta atatgctaac tggtgatata
     7621 gaaatatttg caactgaact tagactttta aacaagtcag aaactccgcc tatttatata
     7681 aaagatgatg atgatgtttc tgaagcacta agattaaaat atagatactt agatttaaga
     7741 aaaccatcta tgcaaagaaa tttaaaatta agacataaag taatgaatat tacaagaaat
     7801 tacttatcaa acaatagatt ttgcgaaata gaaacaccat ttttaatagc tccaacacca
     7861 gaaggagcaa gagattatct agtaccaagt agagttaatc caggaaaatt ttacgcatta
     7921 ccacaatcac cacaattata taaacaatta ttgatggtaa gtggtatgga tagatatttc
     7981 caaatagtta aatgctttag agatgaagac ttaagagcag atagacaacc agagtttaca
     8041 caaatagact gcgaaatgtc ttttgttgag caagaagatg ttatgagtat gattgaaggt
     8101 ttgcttgaag cgatatttaa agaagtttta gatgtagaat tggcacttcc acttcctaag
     8161 atgacttatg cagaagctat gtctaaatat ggttcagata aaccagatac tagatttgga
     8221 tatgagttaa cagatatatc tgatgttgtt tgtaactgtg gatttaaagt gtttgctgat
     8281 gctacacaac caggaaaaag tgttagaggt ataaacgtaa aaggcaaagc tgacgacttt
     8341 actagaaaac aaatatcatc actagaagaa catgctaaga catatagagc aaaaggtctt
     8401 gcttggatga aagtaggaca agaaggagta acttctccaa tagcaaaatt ctttaatgaa
     8461 gaagaaatga acgctatact tacaagaatg aatgctgaag taggagattt acttttattt
     8521 gtagctgata aaaactcaat agtatttgat gctttaggac aagttagact tgaagtagca
     8581 aataggctta atctattgga taaaaatgta tataacttac tatgggtaac tgagttccca
     8641 gtttttgaag aagatgaaga aacaggcaca ttctcagcaa tgcatcaccc atttacatca
     8701 cctatggatg aagatttaga caagttagaa gaaggtgata agtcatcttt aagagcgaaa
     8761 gcttatgata ttgtattgaa tggttatgaa ataggtggag gaagtgttag aatatcaaat
     8821 tctgatgttc aatcaagaat gtttaaagca cttggtttta cagaggaaag agctaatgaa
     8881 aagtttggtt atttattaga agcatttaaa tatggtactc ctcctcatgc tggtctagct
     8941 tttggtcttg atagacttgt tatgttactt gcaggaactg ataatataag agaagttatt
     9001 gcattcccta aaaatcaaaa tgctgtttgc cctatgacaa atgctcctac attggcggaa
     9061 gatgagcaat tagaagaatt aagtataaaa gttgatataa aagataatga ataatatgta
     9121 gttgcaagaa actaaaatgt aaaaagtctg gatataaaat atatttatat tcagactttt
     9181 tttattagaa ttattttata ttttttctct atatattttc ataaattata cttatattct
     9241 taaccaatat aattaaaata ctatgtttaa aaattttaca taaacttgat tattttagaa
     9301 actacttctg taatgagtac acgattttaa ttaaagaata tataacaaat ttgaaataag
     9361 tctaatatat gacttaatag attaaattaa aataataaat ttggactatt tatctattta
     9421 taacttaaat tatatcaaat tgataacaaa ctagatatta tacttttttg tagaatagaa
     9481 ttacaattat ataaagaaaa attctattaa tagtgaaaaa aaagtagaaa aaagtagaaa
     9541 ttattctttt ataaagaaag ggagggataa tattgcaaga gagagaagaa ttagttttaa
     9601 atccagttcc aaaagaggaa agagttggat ggttagctcc attgtttaat atgcttggtt
     9661 ctaacatagc tatatctgaa ctaatggttg gaggaacttt aattttagga atgactcttt
     9721 ctaatatgat tattacatca attataggga acttaatatt agttgcaatt attatgatac
     9781 aaggttatat aggttacaaa gaagggttaa atacgtatgt attagctaag ggggcatttg
     9841 gagagatagg aggaaagtat cttatatctc tattacttgg tataactagt tttggatggt
     9901 ttggtgtaca agcaggggtt gcaggactat cagtacaaaa gatatttcca agtgtaaatc
     9961 taacattagt tacagttata cttggattat ttatggttgt ttttgcactg tatggcttta
    10021 aagctatggc taaatttaat tacttagtta taccaccact tatgatatta atggtgtggg
    10081 gagtttttaa agctttttct acttatggag ttgaagctat ttataattat actcctcaaa
    10141 caactatgag tatggttgaa ggtcttaata ttgtagtagg tcttgttata gttggtgcaa
    10201 ttatatcacc tgaccagtta agatatacga gacgagtaaa agatatttgg attatcagtt
    10261 ttttaggtct agggattatt tcattattcc aacaagttgc agctggtgta atgtctatgg
    10321 gagcacctac ttgggatata acagaagtat tggctaattt aggttttgga tgggtagcgt
    10381 ttgttatttt aatacttgca tcttggtcta caaacgtatc aaatgcttat tctggtggat
    10441 tagcattaaa aactattttt ccaaatgtaa aaagaaatgt tttgacttta gttgcaggtc
    10501 taataggtac aataatagca gctactggta taatatttaa atttcagtca tttttatctt
    10561 tcttgggaat cgcagttcct gcaatagctg gaattatgtg gtgtgagtat tattttatac
    10621 aaggaagaac ttataagcat agagaaggaa taaattggat tgcagtaata agctggttaa
    10681 taggttttgc agcgtcgtat tatagttcaa aaataaattt cttaatacct ccgataaatg
    10741 gaattgtagt gagtatggtt atttattata tattaatgaa atgttttgga atcaaagata
    10801 aataaagggg gcaattacat gggtcgtaag gtgaaaatag gtataattca acaacacagt
    10861 gtacttggag atgttaaaaa aaatatagaa aaggcagtag agatgataga tgacttagga
    10921 aaacaagggg cagatataat ttgtttacca gaactatttg ctacaggata taatttagag
    10981 tctttaggtg gagttaaaac attagaatta ataagagaac ataataagta catagaggaa
    11041 tcaatgtcag aagctgctaa aagaaataat gtatacctga tatctccata tggaacctta
    11101 gaaaaagggt ctacacatgt ttataattca gcagttatat ttgatagaaa aggtaagatt
    11161 atgggagaat actgtaaaaa tcatctgtgg tcattagaag cagtatactt taaaggaggg
    11221 gaaaaagtag aggtatatga tgctgatttt ggtagatttg gtgtaatgat atgttatgat
    11281 gcaggttttc cagaagtatc cagagaattg acattaaaag gttctgaaat aatatttata
    11341 ccatctgcat ggagaataca agatgaagat atgtgggatt taaatgtatc tcaaagagct
    11401 ttagagaaca ctgtatatac agtaggtgta aatttagtaa gtaatgactc taatttaata
    11461 ctatttggaa agagtaaaat atgcaatcct agaggtactg taataactca attagataca
    11521 tacagggaaa aatatgtatt atcagagatt gatttagatg aaataaaaaa gtatagggaa
    11581 gatatcccct atttgaaaga cagaaaaagt agctataaca tataattcta tactataata
    11641 caagttcaat ataaaatgat aaaaagagga tgtatcaata ttaagattaa atttattata
    11701 ctttgatata tcctctttaa tattatcaac tttgatttaa atatttttaa aatagttgat
    11761 aagaaagttt ttgaacaaca acacagcttt agattcatag ttttcgctaa accatgaaag
    11821 atttaaatat cttttaaatt caatatcttt gatatgtaac aacttaatct cagggtcttg
    11881 agataatccc catgattttc cacatataaa tccaactcct tgaagtgatt ttataagagc
    11941 ataaattgta tatggtgaat cagactcaaa tgctattttg ggtttgaaat tagctgattc
    12001 gcacaggata tcaattacct ctctataatt ctcacccttc gttataacta taaagttttc
    12061 atctgaaact tcgcttagat atacttcatc ctttaatgat aaaggatggt ttattgaaac
    12121 tcctaatagt atttcttcgc atgttaaagt tatgctattt tctgagttta gctttgtaaa
    12181 tgaagaagat atgtacaaat caaaatcact ttttttataa gatgatggca aagtatgtga
    12241 cacattaaaa gtaatattgg gatatagttt tctgaaatta ctcaaaagtg atggtaacac
    12301 attagccgct gcaggagata atatctttac ttctcctgaa ttttctaaag aagtgtcttg
    12361 taactccttt tttgaacttt ctactaaatc taaaatgcta tctactcttt ttagaaacaa
    12421 tcttccatac tcatttaaaa caatatatct tccttttctg tcaaataact gaactccaag
    12481 ttctttttct aaggaagata tagttttgct taaagctggc tgtgctatat ttaacttgtg
    12541 tgatgctttt gttatgtgct ccattctagc aacagtttga aaatacttaa gatgcaataa
    12601 atccatagta taaaatacac ctcttttaat atgacttgag ttatatcaat tttataatca
    12661 actccacatt atacattata atataaagtt tatataataa actacaatat attaaaaagt
    12721 tatttaaatt gttatttata ttttgtacat aatctaatta aaaaaagtaa ctttaaatga
    12781 ttaactaagg aaatgttgtt acaaacttta gttatataaa aaattcattt gatgagcaaa
    12841 gtactaatat caagtaagaa taaaagctta ttagataaga aaaataatat tttataataa
    12901 tgatgttact taaattatta tgttatacta ttttaaatag gaaaaattta gatactataa
    12961 ttttaggggg agtcaagatg aaattaacta aatcaatatc atcaatgctg tgcttatctc
    13021 tagttttaag cttatcacca gtagcatatg caaatgaaat aagtagaaca acagttgaga
    13081 atactttagt aggtcaggat agatatcaaa ctgctgttga agtaagtaaa aaaggatgga
    13141 gtagtgcaaa tgaggctgta gttgtaaata gtgaatctat tgtagatgca ttagctgtaa
    13201 caccatttgc taaattaaaa aatgctccta tattattgac agaaaaaaat aatctaaatt
    13261 cacaaactga gaaagaatta aaaagattag gagttaaaaa agtttatata attggtctat
    13321 ctagttctgt atcaaatgat gtgcaaaaac agttagaatc taataattta actactgata
    13381 gagttggtgg tagtgataga taccaaacag ctttgagtat agctaaaaaa atagaaggat
    13441 taaaagatat atcagaaata gctgtagtaa atgggtacac aggtcttgca gatgcagtta
    13501 gtataggttc tgtagctgct actaatggaa tggttatact tccagtatct gatgcatctg
    13561 gtatatctag ctttaaagat tttatagaat ctaagaatat aaataaatca tacataatag
    13621 gctctactaa tgctgtatca gataaaataa aacaaagttt acctaattca gaaagaatag
    13681 gtggagcaga tagaaatgaa actaatggta aagtaataga aaagttttat acatctaata
    13741 aattaaacaa tgtatttgtg gctaaggatg gaatgaaaaa tcaaaatcaa cttatagacg
    13801 ctctagcagt aggagtacta gcatcaaaag aaaattctcc tgtactaatc gtaggagaac
    13861 aattaagtgc aacacaaaag agtgtatttt cgaataaaaa tgctacctct ataactcaag
    13921 ttggtagtgg aggaaatgaa aatgcattta aagaattaaa agatttacag gcaaatagta
    13981 acaataagag agtaatgtat gtaactaata cagataaggt aaatataaga aatgatgcta
    14041 caatagaagc ttctgttata ggatatctaa ataatggtga tgaagtagaa gttttagatg
    14101 tgttaaaaac tggatgggta aaaataaaat acaatgaagg tattggatat gttagtgggt
    14161 cttatttgac aaataataag cctgataata gtaatgaaaa tataaaaata aagtatgtaa
    14221 aagaaaaaga tggtctaaat gttaggaaag gaccaagtac agaagatgaa aaaataggtc
    14281 acctttcata tggaagcaaa gtagaaacaa tagaaatgtt tgctactgga tgggtaaaaa
    14341 taaagtacaa tggtggatat ggttatgtaa gtaatgatta cttatctgat acaccagtta
    14401 tttaattata agtaacaata aaaaaactgc tttaatttgt aaagcagttt ttttataaaa
    14461 atttaagtct aataactatt tctattttga taccttaatc ctgggattta ttattatgga
    14521 tattatcaac atacaaacac tccaaatcca gtgtctaatt ccaaacttag atattaataa
    14581 attcagtcct gctatggtaa ataagcagta tttaattaca attaaaagtg tatttataga
    14641 aagccccctc atagaaagta tcctaaatcc aataatagac attctaagtc ctataaatgc
    14701 caataatata cctgcaatat cctgaattaa gtaatagtat tcaaatttca tattcaatca
    14761 actcttctct gagcagtatc aaatatttct aactcttcaa tttcatcatc tgtgtatgta
    14821 agcatagtaa aaccagttac accataaata aatgaaatta taggtgtaat ccaacttaag
    14881 aaacaatatg gaataaattc tagtggactt acaccaagag tctgagcaca gaaaacagca
    14941 tttgaattcc atggtattaa tggaccacca agtgtagctg tatcttctat aattctagat
    15001 agattttgtg gttttaattt aaattctttg tatattggag acattagagt tccgacaaat
    15061 acagaagtga acatcattga tgaactaaat gcatttgcaa agtagcttac tataaaagta
    15121 gacccaacca atttagttct tgattttcct attttagtta taagaggttg aagcagtact
    15181 tctaagaatc cagttttttg gagaatacca gcaataacga aaactacaaa tactagtaaa
    15241 acggtttcag ccatactcat tattccacca cgatttaata gtttgtcggc atagacaaat
    15301 ccagtatcta tagtaaatcc tgatagcatg taatttaaaa catcacctat tttttctcct
    15361 tcttgaaata ctgctataat aagtcccatg atagctgaac ttagtataga tataatgggt
    15421 ggcttttgta gcaataaaag taataataaa aatattattg gaatcattgc tactaaacca
    15481 atatgaaagt ttgaatttaa tacatccttg atttgattta tttgaacata gtctatagta
    15541 ttatttgaat acttaaatcc aattactgta tacagaacta tacatattac gtatgcaggt
    15601 cctgtggtat aaaacatatg cttcatatgt gtaattacat ctgtcttaca aactgctgca
    15661 gctaagtttg tagaatctga tagaggtgac atcttatctc caaagaaagc accagaaatg
    15721 actgcaccag ctgttaaacc aagaggaata cccatactag taccaatact catcattgct
    15781 ataccagaag ttccaagagt cccccaagat gtaccagtag caacagaagt taatgaacat
    15841 acgattaatg ttgtaagcag aaaatattta ggggttatta ttgttagtcc actatatact
    15901 actgttggta ttgtaccagc agctatccaa gctgctatta gtacaccaac agaaagtata
    15961 attaagtttg attgaaaggc atctttgcca acttcaaatc caaaagcttc tatttcatca
    16021 ttagtataac caagtttcat ggctgcagga actactataa gccatgaaag taaaaacatt
    16081 gtagttatag aagcattaaa tacaactata ccaactgatg ttagagctat tattgcaagc
    16141 attactaaca aggaatatct aaatgaaggt atttttcttt ctgtattcat tttcatagtg
    16201 caaattctcc ttaaatattt ttagttaatt gcatttttta ttcttataag agcttcttca
    16261 agcatacttc ttggacaagc taagtttatt ctttgatacc cacttcctcc aatgccaaag
    16321 ctgtttcctt gatttagagc aactttacct ttttgtacaa gtatactttc tagttcctca
    16381 tcacttaatc caagtgcact aaagtctacc cataataaat aagtaccttc tggttttcta
    16441 actttaagtt ttggcatatt ttcatttata tattttatag caaaatctat attagattct
    16501 aaatattcta aaaatgattc tagccaagat tcaccattat tatatgaagc ttcagttgca
    16561 actaagctaa agcaattatt tctctttata tcaattctgg taaaggcatc atctaacaat
    16621 ttatagtatt tttcatcagg tagtattaca tatgatgatt gaagaccagc tatattaaaa
    16681 gttttagttg gagccataca agtaattgta tttttttcaa attctttaga aatagatgcc
    16741 ataggaatat gcttatgttt ttttaatata atgtcactat gtatttcatc agatatgatt
    16801 tttacattat gcttaagaca aatatctcct agttttttta attcatcttt tgtccatact
    16861 ctaccaactg gattatgagg attacaaagt atgaataatt ttacatcttt aatcttgttt
    16921 tcaatgtctt cataatccat tatgtagttt ccattttcta atttttgtaa tgggcttata
    16981 attagttctc tattgttatt ttttacaaca ctattaaaag ggctgtaaac tggttcttgt
    17041 atcattattt tatcatttgc ttttgtgagt tcatttatta aaagacttat agctgggata
    17101 acacctggac tataaattaa ccactcactt tttattttcc agttatgtct cctatctagc
    17161 cagtttacta tagattcatt gtaagaatct ggtcttgttg tgtagccata aatctcttgt
    17221 tctagtctat tttttaaaga atcaattata caaggtgcag ccttaaaatc catgtcagcc
    17281 acccacatag gaagtaaatc attagttcca tacttttttt ccatttctga ccatttagat
    17341 gaaaaattat tgcttctatc aacaatctca ttaaaattat aattcattgt aaatacctcc
    17401 agtaatttta attttatgtt aataatatta agcaaacaac gtgccaatat tgataataaa
    17461 aaatatttat tcacaagtta atatttaata ttaagtaatt tcagagcata gataagataa
    17521 aaatattaat tatacttaaa actttgtgga aaaggttatt tctaataaat ttttaaatat
    17581 atgaaaatgt aattaaatag gttaaatagg tttaactgtt taacttgtta gacctattta
    17641 tattttttag aaactctact ccctttgatg ttataattga acctccacga ccaacaagta
    17701 tgtctacaag cccaaagtct tgtagttcta aaagcatatt tctaatctgt tgttctgtca
    17761 aaaatctatt ttcctctaaa gctattttac ataagctttt tctacctatt ctttctttta
    17821 aatcataagc cttttttaaa ttatccaata taaatatgta ttcatctagt gaccttttaa
    17881 aattatagtc gtatttcatg atgtttttgt ctttgccaat gttgaattga tactttttta
    17941 tattattact tactttatta caaacggttc tactataatt tgaatctata gtatctaata
    18001 tatattcagg taaatcacat atttctataa tatctttttc caagtaacag aaatattctc
    18061 ctaagtttct taattctctt acatttcctt cccatctata cattttaaag atttctttta
    18121 cttcctcact tagaataaaa ttacatggaa tatcatattt taatgaacca aaaatttcaa
    18181 atatatcagc aggtctttcc ctaagtggat gtattttaag gggaagaaca ttcaacctaa
    18241 aatataaatc tcttctaaac atatttttag atacaagttc ttttaaattt ctattagtag
    18301 ctgcaattat tcttacatca atgtcaatga cattattaga accaatacgt cgtacttgtt
    18361 tttcttgtat gactctaagt aatttagatt gagaatttaa atccatttct ccaatttcgt
    18421 ctaaaaatat agttccattg ttggctaatt caaataaacc aattttccca ccttttttag
    18481 caccagtaaa agccccttca tcatagccaa ataattcact ttgcagtaga ttttcttgaa
    18541 aagtactaca atttactgcg acaaaaggac catcttttct cctagaagca ctatgaatgg
    18601 attgagcaaa taattcttta ccagtaccac tttcaccaat tataaggata gatgaatttg
    18661 attgagccat tttattagca attttttttg tattaatagt ttgtatacta gaacctaata
    18721 tatcatcaaa tgtgtattta ctgatattac cagagtttac tagttgtgct cttaatttag
    18781 cttgcttttt ttctaattga gaaaactttg tcatctttag tataaatcca tcaaatacat
    18841 ttaattgtat atgtttaatc tctaaattta tatcaatatg atttatttta atcaatttct
    18901 caaaaaatgg agttttattg gtcaatatgc tttgaaaatt gatgtctttt atgcaatcac
    18961 caacaaatga gtcaatcatt tcatttgcat ttatagacaa aatttttcta gccatatgat
    19021 tatagaattg aattattcca tcgttatttg tgcatataat tccatcatca attatagata
    19081 gtaacaaatc aaattgtctt tcaaacttat ttgcatctaa caataactca cctgtactat
    19141 aacttgtagg aattattttt tctacgtatt tttttattag gtcatctttt attagatgtt
    19201 ttaatttggt ttttgtagca atttcaacaa tacaacttaa atctattatt ctgtatttta
    19261 aatctataac ttcacaattt ttaaacttag gcaagatttt ttcgccagga gttaggatta
    19321 cagcatctgt gggagtaaac tcaatctctg gatagcaggg caggatattt atattgtcta
    19381 ttcctaatct atgtattaaa gaaattgttt ctattgccat atctttactt aagttatata
    19441 cataaataat tttatctttt tctaatttgg atatcatatc aatactgttc ttttcaaatg
    19501 tcaacttagg aacaatgatt tgagcatcat agtgagataa ttttactatt tcatcatatt
    19561 ttaaataagc agataggagt ataagttttt ctttaaaaaa ctttaaattt ccttcttcaa
    19621 atgagtataa atttatcttt agattatcac ctaagaattg tcttatttga ttttcataaa
    19681 tttctccagc gtcttttttt aaacttacaa tagctaattc catttgtttc attttaacct
    19741 ctcatacaaa tttgacttat tttagattac ataataatta aattagatta aataatatta
    19801 tttaattatt attataaatt tattatagac tacacattga agtaaaagaa gtagctaaaa
    19861 ttattccttt tatttttgtt ttttgtatct atttggtata atagtattag agcgtataat
    19921 aatttaggca aaatttttta aggaagacta gaggagaata taaaatgaaa aaatgtcttt
    19981 actgtaatag ggatataaag tcaccaaata ataaattagc tataaaatat gatgacaata
    20041 tgaatatata tccatgtcgt gcagaatgta aggaaaaaat tgaagaatat atagcaaaaa
    20101 aacctacata taagtttata aatatattag aggttctttt aggtgtgggc ggaatattct
    20161 gctttttgag taaatggact attgcagccc aagttgtact tggaattgat gcactggtta
    20221 tatttttatt cccattggca ggatttaaga tgaatggaaa gttaagtatg gaacaaacta
    20281 agaatcaaag taggtctatt gcaatatcaa ttttggcatt tatagtaata ataactgtat
    20341 tatattttaa acaaattggt aagtagagca taattgtata agacatcatt agagtatgag
    20401 gtgagtttat ggaagaaaga aaattagcac caaacaatga gagggaagct ctcataaaaa
    20461 gattaaaaag aatagaaggt caagttaagg gtatacaaaa gatggtggaa caagaacgtt
    20521 attgtgttga tatattggtt caaatatctg ctataaggtc tgctataaat agagtaggca
    20581 caattatctt agagaatcat ataaaaggat gtgtagcaga aagcattaaa catgatgatg
    20641 ctgataaaac tgaagagatg ataaaagagc ttatggatac tataaataaa tttacaaaat
    20701 aaggaggcgg ttagttatga atcaacaagg atttattatg gaaattgttg atgatagaac
    20761 tgcaaagtta aaaatgcaaa gacattcagc ttgtgctgct tgtggaaaat gtgctactac
    20821 aaattcagca gagtcaaaag aaatattagt agaagtagac aacacaattg gcgcaaaagt
    20881 tggtgaccat gtagaagtga gtatggataa tatgaatgta ttaaaagcag ctgtaatggc
    20941 ttatataata cctcttatag cacttcttgt aggaacaata gggacttatt atatattggg
    21001 ggcaattgga atgacaactg gagttgaagc cataagtgga ttagttggaa tttgtcttac
    21061 aataatctgt tatttatact taaagaagaa tgacaaaaaa tttagagaca gtagagaatt
    21121 tataccagta ataacaagaa tattaattga cctttaagat aattataatt aaaaaatagc
    21181 aatgtgcttc ttaaataaat actcaatacg tatttgtaga gtttaagtac attgctattt
    21241 ttatattttt attttgattt tctgtaagga ataaagtttt ttctgatttt caaaagattc
    21301 aatgttctta caaaagaagt tacaactaat acaccaagtg ctattgcagc acttagaaat
    21361 agcgcatctg taccttttga catagccaaa gaataatttt tctgaatcaa agacaacatt
    21421 gtattatata gcccaattcc tggtattaga ggaagaatac ctggaattac gaacacaata
    21481 gctggttgct ttagttttct tgctaaagtc tcactgcacg ctgagaccaa agcagcagcg
    21541 ataaaacctg caaaaactgc atttgtagta aaattaaata aataaacata tacaatccaa
    21601 ccaattccgc cagttactcc agctggaaga agtagatata gtggagcatt aaagaaaaca
    21661 gcaaatccag cagttgcaac acctgaaaat aaaaaatgca aaatcataga catatcagtc
    21721 attagaaagg acctcctatt ctataccatg tatcaaggac aaaaccaact ccagaagcaa
    21781 ttgcaataat aatcatagca gcatcaaaag cacgagaaat tcctgatatt aaattccctg
    21841 atattaaatc tctaatagct tttataaaag caactcctgg gagtagtggc attatagcgc
    21901 cgactataag cattttaggg tcatctaaaa tgcctatttc aactagtaga actcctgaaa
    21961 gtgcaattaa aaaagaagat actaaacagg aaaatgcaga tattgcactt atcttcatcg
    22021 ttttatcata agtaattgca gcaaagattg ctattataaa cgtaagtata aaatttaata
    22081 cataattccc tccaagcaaa caagcaaaac aagcagatgc aattcctgta caagagtaaa
    22141 ataaccataa aggataaggt tttacatctt ggatttcata aagacgtgca atggcattat
    22201 caatatcata ctctttgtca ctaacaaatt ctcgagaaaa actgtttaat aaagaaatct
    22261 tatttaagtt tatacctctt gattgaatcg ttttcataaa cgaaaagcca tcaaatttct
    22321 catctgaaat aattattact gtaggagttg taaaaatatt tacatgataa aaccctcttg
    22381 acctgcatat tctaaggaca ctgtcttcaa ccctagatgt ttcagctcca ttttttatca
    22441 tcagttcccc tacaaataga gctagtttta gaatattttt cttatattca agttcctcat
    22501 tttgattcat aaatacacct ccaaattaaa aaacgtaata ttgaaattat atcaaatttt
    22561 taagtaatat tccttaatat ctatacaaat atattatctt ttttttgttt caaaatagtc
    22621 acatttaaat tatatccaat taaaagaatt agacaatatt atttattata ttttaactta
    22681 aaaataaaga aagtaactat gttaaatatg gaaaaatggc aaaacacttg actattaatt
    22741 ggtaaaggac tataataata aattatcata ttgataaaaa cgaaaattat gaaatgtctg
    22801 taaaaataaa atttaaataa taactaggag gagtaatatg tttgagaact tgaaaaaagc
    22861 ggaccctgag atttatgaaa gtatgaaaag agagttaaaa agacaacaaa gaaatattga
    22921 attaatagca tctgaaaaca tagtatcagt tccagtaatg gaaactatgg gaagtcatct
    22981 tacaaataag tatgcagaag gttactcagg caaaagatat tatggtggat gtgaatacat
    23041 agatgaagtt gaaactcttg caatagatag aatcaaaaaa atatttggag cagaacatgc
    23101 taatgttcaa cctcattctg gagcaaatgc aaatattggt gtatattttg ctatgttaaa
    23161 accaggtgat gttgttatgg gaatgaattt atctcaaggt ggacatttaa ctcatggtgc
    23221 tccagttaat atttctggtc aatattataa attttatgaa tatggtatag ataaagacag
    23281 tggacttata gattttgatg aagttagaaa gatagcacat gaagtaaaac ctaaaatgat
    23341 agttgcagga gcaagtgcat atcctagaga aatagatttt aaaaagttta gagagatagc
    23401 tgatgaggtt ggagcattgc ttatggtaga tatggctcat atagcaggtc ttgtggctgc
    23461 tggtcttcac caaaatccat gtgaagtggc tgattttgtt acaacaacaa ctcacaaaac
    23521 tttaagagga cctcgtgggg gtgttattct ttgtaaagaa aaatatgcta aagacataga
    23581 caaagcaata ttccctggta ttcaaggtgg acctcttgaa catataattg cttctaaggc
    23641 agtttgcttt aaagaagctt taagtgatga gtttaaagaa tatcaggtac aagttgctaa
    23701 aaatgcaaaa gccttagctg aagaacttat taaaagagat tttaaattga tttctggagg
    23761 tacagataat catttaatac tattagattt gactaataaa aatataactg gtaaagcagc
    23821 agaaaagaga ttggatgatg cttatataac agctaataaa aatacaattc catttgaccc
    23881 aaatggtgct cttgttacaa gtggaataag acttggtact ccagctgtaa ctactagagg
    23941 tatgaaagaa gaagatatgg caataatcgc tgaggctata gacctatgtt taacttatga
    24001 tgaagaatct aaggctagaa cattggttgt tggtttaact gaaaaatatc ctttatatga
    24061 agaatataat attatggatt aattataaag aacaactaaa taaaatacat ttaaataatt
    24121 acaatgattg tgtatgtatt tgaatatatt tagatataaa aaaggtgtct caaaatgaac
    24181 tttttagttt atgaggcact tttttgtatg gaatcgaaca tatttgtatt atttaaataa
    24241 taaaaaatat ggttatctct atttttagat ggtctctttg tattttttaa ttaaaacttt
    24301 aagatttctt aagaattata ctaggttttt ctaaagattt aaagtctata cttataagta
    24361 tagtaatcat atgaaaagaa aagctttaac gaggagggaa tcttatgaag gtattaattc
    24421 ttacaggaaa atttggtatg ggtcattact cagcatcaaa ttctttaagt gaagatataa
    24481 aagcaaaatt tgataattca gaaataataa ttaaagatat atttgagtac attatgccta
    24541 actattcaga taaaatgtac aagacttttt ctattcttgt aaatcgggga agtagtcttt
    24601 ataatttgtt ttataaatgt gctgaaaatg gaaagaaaga tataaaattt actttttcag
    24661 attatttttt aaataaatta gatacattac ttcatgaagt acaaccaaca gtagttattt
    24721 caacttttcc attttgttct caattagtat caagatataa ggaaaaatat aactcaaatc
    24781 ttcctttaat aacatgtata actgatataa gtagtcactc agaatggatt agtaaaaata
    24841 cagactgtta tttagtagct agtaaatcta ctaaggaaga acttgttttt aaaggaatag
    24901 atgaatctaa gattaaagtt aatggtattc cagtaaaaaa agaatttaaa agaatagaac
    24961 atgtaaatca ttcaactaaa aaaaatattc ttataatggg tggaggatta ggattactgc
    25021 ctaaatcaga acaattttac aaagaattaa atagtttaga aggagtaaaa actactgtaa
    25081 taactggtaa taataaaaaa atgtactata aactatatgg tagatatgaa aatatagagg
    25141 tagtaggtta caccaatgaa gtatataaat atatgaaaga ttctgacctt attatttcta
    25201 agccaggtgg aataacacta tttgaaacaa tttattcaga acttccaata ttggcattta
    25261 atccattttt acaacaagag atagataatg ctagttttat attaaataat gaaattggga
    25321 gaatacttgg taaaaataaa aaatactatg tagatgagat aaaagattta atttatgatg
    25381 atgctactct taaagaaatg agcagtaaca tgaaagaatt aaaaaaacaa tttgataata
    25441 ataccttaga gaatatttta ttttcacttg atgaacaagg agcgtgtaga gaatgtatgt
    25501 agtaggttta attatcgttg ttgcattgat ttttttagtc cattcaatta tacctacata
    25561 ctataataaa ctattaaata aagaggtatt aaaaaatatg gctggagaaa atgaaattgc
    25621 acttacattt gatgatggtc cagataagag atatacagaa aaattactag atgtgttaaa
    25681 agaaaatgat atacaagcta tgttttttgt agtagcaaag aatgctgaaa aagagcctga
    25741 aattataaaa agaatgttga gagaaaatca tatagttggt ttacattcat tggaacacag
    25801 aaatgcatgg ctttatagtt atagttatgt taaaaaagat tttattgaaa gtacaaatat
    25861 aatgaaaaat ttaggtgtag atgtaaacta ctatcgtcca ccatggggtc atactaatat
    25921 attttcaaat tcatttgtta aaaaatataa tctaaaaatg actttgtggg atgtaatggc
    25981 agaagattgg gagaaagata gtacagtaga cattattata aacaagttaa tgagtagaac
    26041 aaaagaaaat tctataattt gtttacatga tgcaggtgaa aattcaggtg gtgcagtagg
    26101 tgcacctgag cgtactattg aagctttaaa gatagctatt ccaaaactta aagccagtgg
    26161 tttaaagttc gtaacaccag aaaggatgta gttatgataa gtaaaactga aaagaaaaat
    26221 aaatgcctag gtagtgtagc ttttttagtt ttactaatgg gcataacagg atattttgtt
    26281 tttagagggc agtcagttga atcattgata aaatcattaa aaggtgcaag ccctatgttt
    26341 atactaattg gatttgcaat gatgtttata tatgttgcct gtgaaggtat aaatatatac
    26401 ttaggtatga aagctttaaa ccaaaagact actcttttaa aatgtatggg atatgctttt
    26461 attggatttt attttagttc tattacgcct tcagcatcag gtggtcaacc agcacaagtt
    26521 tattatatga aaaaagacga tataaatata tcatattctt cacttatact attagtcatt
    26581 gttgttattc accaagtagt tatactggca tatagtggaa taatgttcat tatggaacgt
    26641 gagtttatat taaataatgt aagtggtatg aacattttac taatatatgg tgtaataaca
    26701 aatgttgctc ttgtcattgg agtaattgca attatatttt caaaaaaact tgtaaacaat
    26761 tttataatat caataacaaa tttattaggt aagttaagaa taataaaaga tgttgaaagt
    26821 tcaagaaaag ttataatatc tcaaattgaa gagtatgtta aaggtgctca atatataaag
    26881 cagaatccaa aacttgtagt ccaaatttta gtcataacta ttgtacaaat aacatctatg
    26941 tttttagttc ctttctttgt atacaaagct tttcatctat caacatatac tgtatttgag
    27001 atattagcga tacaatcctt gcttaatatt gctgtttctt cattaccatt gccaggggct
    27061 gttggagctt ctgaaaatag ttttatgaca ttatttaaga tatttttccc tggtcactta
    27121 ttagttccgg caatgttact tagtagggga ataagttttt atgcatttgt agctataagt
    27181 ggattgatat gtatagtagt acatgtaaaa tcttctgaaa aagtaaaagc agtgaaaaaa
    27241 gttgcttatg taagataata ttatatagat tatttttatt aatatgtcaa tttgtgtgta
    27301 tattttaatt aagctttaaa catgtttaca ggagtagaaa ctattaaaac gtgtgacatt
    27361 gctaagcttt tgctaataaa gtataatatt ttatatagtt tgaaatattt aagaaatata
    27421 gcaaaaaaag gagcttggtg atgaataaag agattccaaa aaaaatttta gtagctgatg
    27481 ataatcctga gattagggaa atcgtagaaa tacttttaac tggtgaaggt tatgaagtta
    27541 ttatggcaac gaatggagaa gaagctgtaa acttagtaga tagtacgatt gatttgatta
    27601 ttttggatgt tgtaatgcct gtaaaaacag gatttgttgc atgtaatgaa attagagaaa
    27661 aaacgacagc accaatatta tttttaactg caaaaactca agactctgat aaagttattg
    27721 gattttcagc aggaggagat gactatttat caaagccttt ttcatattct gaactaattt
    27781 caagggttaa gtcgctactt agacgttatt atatatacca gggaaaggaa aaggaaagag
    27841 agccaaatat attaaaaaca aaagagttat gtgtcaattt agatactcaa gatgttacac
    27901 taaatggaga acctgtgttt ttgacaacta tagaatatag tatattaatt cttatgttga
    27961 aaaatagaaa aaaagttttt tcgtctgaaa atttatatga aactatttgg gatgagcagt
    28021 atttttatac agcaaataat acaataatgg ttcatattag aaatttaaga aaaaagttag
    28081 aaaaagacca aaaaaatcca caatatataa aaaccgcatg gggaaagggg tattatattg
    28141 attaacaaaa ttaagccatc taagttggga gttatactat tgatatatgt agtaatctca
    28201 gctgcagtgg ctttcatttt aaatatacca ttttactctg ctgttgaatc tatagtaaaa
    28261 gatgtatcag aagggaaagt atattttaaa agagaagtaa atcaacttat ggataatttt
    28321 caaacttatg tgaataacaa tggtataaga gcagatgatg gagatgatat agcaaaatgg
    28381 aatagtgata attggtttgt ttctatgaag atatataaag gaaataaggt tttttatgat
    28441 acattatact atcctaatga agtacctgat aagagtgagg aaaatgagtt ttatagcagt
    28501 cccatggcta gaaactatga gatacaattt tctgatatta aagctaatat agttgtagta
    28561 gctttattta gatttagatt tcaaaatttt atagaagcaa tttctattac atttatggca
    28621 cttacattta ttataacatt tttactgttg tttagtagaa agataaagta cctaaggaaa
    28681 atagaaatgg gtataaagat agtagagagt ggcagtttaa agtatagaat cccagtaaaa
    28741 ggtaatgatg aattatcatc acttgcatct agtattaatg agatgagtca tacattggag
    28801 aatcaactta taaaagaaag ggagatgaag aataaaaata aagatatagt aacttcgata
    28861 tcacatgatg ttagaactcc attaacgtca gtaatttgtt atttagattt gattaaagac
    28921 cataaataca atagtcctga gcaattggaa caatacataa aaaatgtaag gtctaaagca
    28981 tatcaaataa aagatttaac agatgacctt tttacgcatt ttgttaattc tggagaggaa
    29041 gtttcaaaag ggtatgaaaa aataaatgct aatgaattta tttttcaatt acttttagat
    29101 agttcatata cattggagga gaaaggattt catataacaa tagaaaatga actttataac
    29161 caatatcata tttatgtaga tgttacacaa tttagaagga tatttgataa tttatgttct
    29221 aatataataa aatatgctag gaaagataag cctatagtat ttttaattaa agtagaggat
    29281 tctaaattac taattataca aaaaaataga gtaagtaatt tttctaagaa tgtagaaagt
    29341 tttggaattg gtataaagaa ttgtgagaaa atagttgcat atcataatgg aagcataaat
    29401 gtaaattcag atgataaaga atttgctatt gaaatatcta ttccagcaaa agaatcataa
    29461 aaatttaaat tatatattgt gtaattgcta ttatatattg tgtaattgct tatgtaaaat
    29521 tctatgaagc aggaagcatg tgactttagt tatgggatgt tcaatattgt gtacctctag
    29581 gcaagtaaca agtttactta caagaccata taatattatt atcttaaaaa aaggggacat
    29641 aataatatgg ttttgacagg agcacattat atttacattc tatttatggt aattatatta
    29701 attactatga ttatgaaaaa ggatacaata gtaccatgta tattaggtgt tttttttatg
    29761 ggattgttct ttgaaaaaaa catctttggt gctataacag cagtgtttaa ctcttttatt
    29821 atatctttaa atgagttggg tccaattatc ttgattatag ctattatggt tgcattatct
    29881 aaagccctag aagccaataa tgctatacag tatatggtga gaccattttc gagagttata
    29941 aaaaattcta atacagcatt ttttgtaact ggatttgtta tgttagttct ttcttggttt
    30001 ttctggccaa ctccagctgt tgccttagtt ggagctgtat ttttacctgt tgctatgaga
    30061 gctgggctac cagcaattgg tgttgcagta gcattaaatt tatttggtca tggtcttgcc
    30121 ttatctacag actttgttat tcaaggagct ccaagtataa cagctggtgc agcaggtgtt
    30181 gcagtatcat atgttataaa tgatggtatg attttgtttt gggtgatggg aatcgtaacc
    30241 atatctatgg cattttatac attaaaaaga gatattaaca aaggaatgtt tagagaagaa
    30301 ctaaaaggtt ttgaaagtga agaagtagaa gcgttcaatg gaaaatctaa aatagcaact
    30361 attttagtag ctttgggatt tcttgctgat ataattgcta tgtatgtatt tgacctaaaa
    30421 ggaggagatg catcagctct tttgggtgga acagcagtat ttttaataat aattataaat
    30481 gttataaatt ttggcaaaga tagtcttgaa aatgtatgtg agaatataat tgatggtttt
    30541 gtgtttggga taaaaatatt tggagcaatt ataccaatag cagcattttt ctatatgggt
    30601 gaagttgcac cattgacagg tgtatttgga aaagtattag caccaggttc acaaggttta
    30661 ttatcagata taggcatagc tctttctcaa acaataccac ttaataaatt tgccgtatca
    30721 ggtatagaaa ctgtagtggg agcaataact gggcttgatg gttctggatt ttctgggatt
    30781 tcattagttg gttcacttgc ctctgtattt ggaactgcta taaatgcaag tgttggtgca
    30841 ttagctgcat taggacaaat cagtggtata tgggtaggtg gaggctgtct agttccttgg
    30901 ggtcttattt cagcagcggc aatttgtggg gttagtccaa tagaattggc aaagcgaaac
    30961 tttgtcccag ttataaccgg attggttgta acaaccatag tagcaatgtt tattatttaa
    31021 taaatcgaat aaatctttaa ttgtataaat taaaaataga tgtgtagcta taatttatta
    31081 taattgtttt actaattcta agccatacat ctatttttat tgatattttt attagtattc
    31141 tttaataaag taatattttt cattatatta tacataacat ctaaatataa catctaaaat
    31201 actgttatct atatttaata tcttgccaaa ttttttgatg ttttattgcc ctgtcccacc
    31261 aacatatata agctatcaca agtttttacc tatactatca ccaaacaaaa attctaattt
    31321 taatttatta ttttataacc tatattatat taactatatt agtttgtaga ctttagatgt
    31381 aattataaat attaatactc caagagttca aattcatatc ctagttttct tgcatttgat
    31441 ataaaatcat ttaaaatttc tgtactagtt ttggaaactg catgtagtaa caagatagaa
    31501 ccatcatgta gattatccat tattttttgc gttgcttctt catgtgatgg ttgtttatca
    31561 gtatcccagt ctctgtatgc aaaactccaa aatacagtct tgtatcctaa atttttagtc
    31621 attgccaagc ttttttctga gtattttccc ataggaggtc taaaaaattt aaccatatct
    31681 ttcccagtta catctttata taacttttca acatcatata attcgtcatt aaagtttttt
    31741 agattgcttg tttttgttgg catagatgga tgggttttac tatgatttcc aactatatgt
    31801 ccctcagaaa ccattctttt tactaaatct ttattttctt ttatatatgg agctgtaaca
    31861 aaaaaaactg ctttaacctg gttttgtttt agagtgtcga gtatttttgt agtgtatcca
    31921 ttttcataac cttcatcaaa tgtaagatat agtgtttttt gaccatcttt tgtaggtcca
    31981 ttgtataggg catcatagtc agaaaattta aattcaatgt ccgtatttac ttctggtgtt
    32041 ttatgcttat tgtttggtat ataaaaccaa tcaagagtag tgttatctaa agaagataca
    32101 tctaatttat tattatcatt attagtagtg gcttttgatg gtttatctgt tccgtctgaa
    32161 actattacat tggaagtatc tttaccacta tctatttttt ccttatcttc ttgtaactgt
    32221 gtttccttat tttgattttc gttttgattg ttttgtgaat ttgaacatcc aacaataaaa
    32281 aaaatagaaa tagaaagtag tgttaaataa tgcaatactt ttctaaacat tttgaagctc
    32341 ctttctaaat taataataat tgcatataat aataattata tgattaatta caattgcata
    32401 tagtattatt ataattggaa attactatta ataatagtta tatctttaaa aataaagtca
    32461 caatagtatg acaaagggtg aataatcaat aaatttaaaa aaaaatgaaa aaaatagttg
    32521 accatattat gagaagatgt tattataata aatgtgctca acaagaagta acaaaataac
    32581 aagcacaaag aagttaattg aat
//
LOCUS       DBGJFU010000034        32332 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.034, whole genome shotgun sequence.
ACCESSION   DBGJFU010000034 DBGJFU010000000
VERSION     DBGJFU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.034"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            267..2366
                     /gene="nrdE"
                     /locus_tag="ACOIAS_001533"
     CDS             267..2366
                     /gene="nrdE"
                     /locus_tag="ACOIAS_001533"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGZ2701248.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2378..3349
                     /gene="nrdF"
                     /locus_tag="ACOIAS_001534"
     CDS             2378..3349
                     /gene="nrdF"
                     /locus_tag="ACOIAS_001534"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGZ2701249.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3425..4051
                     /locus_tag="ACOIAS_001535"
     CDS             3425..4051
                     /locus_tag="ACOIAS_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2701250.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            4446..5075
                     /locus_tag="ACOIAS_001536"
     CDS             4446..5075
                     /locus_tag="ACOIAS_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701251.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            5076..5858
                     /locus_tag="ACOIAS_001537"
     CDS             5076..5858
                     /locus_tag="ACOIAS_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701252.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            5851..6729
                     /locus_tag="ACOIAS_001538"
     CDS             5851..6729
                     /locus_tag="ACOIAS_001538"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701253.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            6762..7721
                     /locus_tag="ACOIAS_001539"
     CDS             6762..7721
                     /locus_tag="ACOIAS_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2701254.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            8035..8727
                     /locus_tag="ACOIAS_001540"
     CDS             8035..8727
                     /locus_tag="ACOIAS_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2701255.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            8968..9741
                     /locus_tag="ACOIAS_001541"
     CDS             8968..9741
                     /locus_tag="ACOIAS_001541"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2701256.1"
                     /translation="MYLGSSFLGRNISVILKQQHKLYQSMIQEHKKIHSEHLTFINQW
                     IHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLDMAMHFARADNFQKDFIVEK
                     AKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDVKWMKFIIGQLITNGVKYSK
                     DYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFAPFFTGENGRKFGESTGMGLY
                     LVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKGVNSLIKM"
     gene            9989..10840
                     /locus_tag="ACOIAS_001542"
     CDS             9989..10840
                     /locus_tag="ACOIAS_001542"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGZ2701257.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            10944..11690
                     /locus_tag="ACOIAS_001543"
     CDS             10944..11690
                     /locus_tag="ACOIAS_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701258.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            11690..13606
                     /locus_tag="ACOIAS_001544"
     CDS             11690..13606
                     /locus_tag="ACOIAS_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2701259.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            13888..14802
                     /locus_tag="ACOIAS_001545"
     CDS             13888..14802
                     /locus_tag="ACOIAS_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HGZ2701260.1"
                     /translation="MILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINSTP
                     GIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLNT
                     GEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIVH
                     PYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSIG
                     IYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWILS
                     MGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            14880..15617
                     /gene="cas6"
                     /locus_tag="ACOIAS_001546"
     CDS             14880..15617
                     /gene="cas6"
                     /locus_tag="ACOIAS_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HGZ2701261.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            15623..17473
                     /gene="cas8a1"
                     /locus_tag="ACOIAS_001547"
     CDS             15623..17473
                     /gene="cas8a1"
                     /locus_tag="ACOIAS_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HGZ2701262.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            17475..18389
                     /gene="cas7i"
                     /locus_tag="ACOIAS_001548"
     CDS             17475..18389
                     /gene="cas7i"
                     /locus_tag="ACOIAS_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HGZ2701263.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            18391..19092
                     /gene="cas5b"
                     /locus_tag="ACOIAS_001549"
     CDS             18391..19092
                     /gene="cas5b"
                     /locus_tag="ACOIAS_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HGZ2701264.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            19103..21424
                     /locus_tag="ACOIAS_001550"
     CDS             19103..21424
                     /locus_tag="ACOIAS_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HGZ2701265.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTYLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            21472..21990
                     /gene="cas4"
                     /locus_tag="ACOIAS_001551"
     CDS             21472..21990
                     /gene="cas4"
                     /locus_tag="ACOIAS_001551"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HGZ2701266.1"
                     /translation="MPIDLEVLKVQGVKINYYYVCKRKLWLFSKGITMEQNSSRVLSG
                     KIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEMQLYYYLYYLKN
                     SMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSPPKIEKKHYCTK
                     CAYYEFCFVKEE"
     gene            21993..22973
                     /gene="cas1b"
                     /locus_tag="ACOIAS_001552"
     CDS             21993..22973
                     /gene="cas1b"
                     /locus_tag="ACOIAS_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HGZ2701267.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            22976..23242
                     /gene="cas2"
                     /locus_tag="ACOIAS_001553"
     CDS             22976..23242
                     /gene="cas2"
                     /locus_tag="ACOIAS_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HGZ2701268.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   23426..25154
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=23426..23454
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            25215..25643
                     /locus_tag="ACOIAS_001554"
     CDS             25215..25643
                     /locus_tag="ACOIAS_001554"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGZ2701269.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(26098..26877)
                     /locus_tag="ACOIAS_001555"
     CDS             complement(26098..26877)
                     /locus_tag="ACOIAS_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701270.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            27201..27746
                     /locus_tag="ACOIAS_001556"
     CDS             27201..27746
                     /locus_tag="ACOIAS_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HGZ2701271.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            27778..28914
                     /locus_tag="ACOIAS_001557"
     CDS             27778..28914
                     /locus_tag="ACOIAS_001557"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HGZ2701272.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            28918..30309
                     /locus_tag="ACOIAS_001558"
     CDS             28918..30309
                     /locus_tag="ACOIAS_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HGZ2701273.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            30385..31260
                     /gene="dpsA"
                     /locus_tag="ACOIAS_001559"
     CDS             30385..31260
                     /gene="dpsA"
                     /locus_tag="ACOIAS_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HGZ2701274.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            31263..31853
                     /locus_tag="ACOIAS_001560"
     CDS             31263..31853
                     /locus_tag="ACOIAS_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HGZ2701275.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(32060..32260)
                     /locus_tag="ACOIAS_001561"
     CDS             complement(32060..32260)
                     /locus_tag="ACOIAS_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701276.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT        12961 a         3104 c         5448 g        10819 t
ORIGIN      
        1 gagttgataa aaatttcttt aaataaaaat aatgtcaatt ataaagtaat aaaagtttag
       61 atataaaaaa atttgtgtat atactatatg tagtatataa tagataaagc actgactata
      121 tgtagtgctt ttgtttttga aaaatactta ttgcatttct ataattatag attaattttg
      181 ggatttcaac tgataagtac caaaagaaat aataagcaaa ttaagtgaaa ttatggcaca
      241 agattatgtt aaaaggacgg taaataatga attggataga gcttaacaat caagtgataa
      301 ttaaaaatga acaaggcaag taccaattgg aaaaggataa ggaagcctta agtagttaca
      361 tagaagaatt tatacagcca aagttgagaa aatttaataa cctagaagaa agattgaagt
      421 atttaataga tgaaggttat tattcaaaag aagtaataaa tcagtatagc atagatgtta
      481 ttaaaaatat atatgatatg attgataaac ataagtttga atttcaatca tatatgagtg
      541 caaataagtt ttatcaaaat tatgcattaa agagcaatga tggaaaggaa atattagaaa
      601 catataatga taaagtgtta attgtagctt tgacactagg aaatggtgat aaagatttag
      661 ctttaaactt ggcagataaa atagtaaaac aagaatttca accagctaca cctacttttt
      721 taaatgctgg aagaaaaagg gctggagaaa tggtatcatg ttttttacta tctgtagaag
      781 atagtacaga aggtatatca tatgctatat cttcatcaaa tcacttatca aagattggtg
      841 gaggagttgc actaaattta tctaaactta gagcatctgg cgaatctatc aaagatatag
      901 aaggtgcagc tggaggtgtt gttggagtag ctaaaatgtt agaacaatca tttagttact
      961 ttaatcaaat gggagctaga caaggttctg gtgcggtgta ccttactgta ttccatcctg
     1021 attttgaatt gttgatggat actaaaaaga taaatgcaga tgaaaaaata aggcttgcta
     1081 cattatcatt aggagctata ataccagata aatttatgga gttggcagaa aaaaatgaag
     1141 tagcatatgc attttatcct catactgtgt ataagaaata tggagtttct cttgatgaga
     1201 tagaaatgga tgagtggtat gataaattgg ttaatgatag tgatataaga aaaaaagaaa
     1261 taaatcctag gcagatgttg acaaaaatag cacagatgca acaagaaagt ggatatccat
     1321 atgtagtgta catagataca gctaatagag aacatacatt aaaagatgtt ggaattatta
     1381 aaatgtctaa cttatgttgt gagatatttc aatatcaaac accttcagaa atagaggggt
     1441 atggaggaaa aaatgaatgg ggacaagata taagttgtaa cctaggttct ttaaacatag
     1501 ctaatgtaat ggataataaa actatagaaa gtacagttga aacagcaata agagcactgt
     1561 catttgtagc agatagcaca gatataaaac cagtgccaac ggtaagcaat agtaacagca
     1621 agtctcattc tataggatta ggagctatga atttacatgg atacttagta agagaaaata
     1681 ttttatatac ttcagatgat gctatagatt tttctaatgt attttttgca atggttagat
     1741 actattcaat taaagcttct atgaaaatag ctatagaaag aaatcaaact tttgaaggat
     1801 ttgataaatc agaatatgtt aagggaagaa atagcaaggt tttatcaaaa tactatgaac
     1861 aatcttatct acctaagtca gaaaaggtaa gagcattatt tgaagggatt tatataccaa
     1921 caaaagaaga ctggacaaaa ttattggatg aggtaaaaga aaaagggata tataatgcat
     1981 atttaatggc agtagcacca actcaaagca ttagttatgt tcaaaatgct acatcaagta
     2041 taatgccaat tactgagcca gtagaagtaa gaacatatgg agactctact acaatatatc
     2101 caatgccatt tttaacaaat gataatatgt tgtattatca atcagcatac aggatggata
     2161 tgagaaaggt catagattta gtggcaacag ttcaaaacca tgttgaccaa ggtatatcaa
     2221 caacactgtt tgttacagat gaaaagacaa ctagagatat agctagacat tacatatatg
     2281 cctataaaaa aggtttgaaa agtttatatt acacaagaac aaagatgaca agagatactc
     2341 atgagtgtct agtgtgttca gtatagggag agaaattatg acatttaatt tacataaaat
     2401 acataatgca gttaactgga atagagaaga agatggattt acacaagctt tttgggaaca
     2461 aaatgtaaaa caattttggc ttcctgaaga aatatctgtt tctaaagaca taaaggtatg
     2521 gaatgaacta agtaataaag aaaaggaatt atataaaaaa gtattgggtg gtttaacatt
     2581 actggataca aaacagggga ataatggaat accatctatg atgagtttaa cagaaaacct
     2641 acaaagaaaa gctgtcttgt cttttatggg gactatggaa gaaatacatg caaaaagtta
     2701 ttcttctata tttatgactc tactttctaa tttagaaata gatgaattat ttgaatggat
     2761 agaaacagaa ccaacattac aaagaaaggc tgatttagtt ttagctcaat acgaaaatac
     2821 tacaaatcaa gagggattat acctatcaat ggttacaagt gtatttttag agagtttctt
     2881 gttttattca ggattctttt atccattgta tctatctggt caaggaaaga tggtagctag
     2941 tggagaaata atatcgctta ttcttagaga tgagtcttta catggtaagt atataggatt
     3001 gctagcacaa gaaatatacg attcatttga taaaatggat aaggagatgt tagaagagaa
     3061 gatgtattct atactttata gccttatgga gaatgagata gagtatacaa atgttatata
     3121 cagagaaagt gggcttgaaa aagaggttgt gaacttttta aaatacaatg caaatagagc
     3181 tttagaaaat ctagggtttg agaagctata tactgtagat gctataaatc caattgtatt
     3241 aaatggtctt agcactgaaa ccaaaacaca tgattttttt tcaactaagg gtaatggata
     3301 ccaaaaagga gtgtatgagg aattagagga tgaagatttt ataatataat ttataattat
     3361 gacacactgt tgtcactttt agcgtgataa tatcaatgta taaaatatat atggaggtat
     3421 ttttatgaaa tatgaatgga gaaaaagaga taaagagtta tatttaccta aaaagaatcc
     3481 tgtaatagtt gaaataccag aacagaaatt tatcatgtta aaagggagtg gagacccaaa
     3541 ttctaaagaa tttactgaag caataggagt actttattct ttggcatatg caataaaaac
     3601 catgcctaaa aaaggaattg taccagaagg gtattttgac tatacggttt ttcctttaga
     3661 aggtatatgg gataagggag aaaaaagtaa aggcttagat gtacttatta aagaagattt
     3721 aatttacact ataatgatac gtcagccaga ttttgttaca caagacctgt tggataaagc
     3781 tactgatata gtaaagaaga aaaaacctca taatcttctt gaaaaagtta gatttgggtc
     3841 tattaatgaa gggctatgtg ttcaaatgct tcatataggt tcatatgata atgaaccaga
     3901 aagctttgac attatgaacg aattttgcaa aaaaaataaa cttataagaa aatcttacac
     3961 acatcgtgaa atttatataa gtgatgctag aaaagtatcg cctgaatcat tgaagacggt
     4021 tttacgtttt gaagtagaaa gggaagtata aatattttga ataatttata agagttataa
     4081 aaagtgaagt ctgttgagac ttcatttttt gatgcaattt taaaattaaa tttgatgttt
     4141 cataatattt taaaaacttt ctacttttat tagtgtgaat aactgttgct attaaataat
     4201 aatactaatt atataagtat tattatataa tatttataga ttattatata aatagattat
     4261 tagtaagatg tatgcattat taattaaaat aaattacata tactatttaa tatttcattt
     4321 aaaatattaa aaaatgtaga taaaaattgt tttattaaga aaaaattaat atattgacaa
     4381 aatttaagtg ctcatttaaa ctattaattg ataataatat tcaatattaa ataataggag
     4441 gcgttatgaa taaatatctt ctaataaatc cagtggcaga aagaatgtat ggagaaaagt
     4501 ttcaccttat taaagcaaat ttgataaaaa aggggtacat aataacagaa tgtgaaccac
     4561 agttagaata tgtaaaaaag cagtacaaag agtatacaaa aaagactgaa aatacaatgt
     4621 tggattgtag atgtccagaa agtataagtc ttttaaaaag aaataatttg ataaataatt
     4681 ttgaagtacc tatgatagaa ccaatattag taaggacttg tagggtttta tacgataaat
     4741 atataaaaag taaagatgat atgttggtag taacctgtcc atgtacacaa ttaagagact
     4801 ttgcaagtga aaagtttaag gataagggta atgctatgtt ttatacatgg aaagagtttg
     4861 ctgaacaaga aggtgtaaaa tcacttggaa aaatagatga atcaccaatt ccattaggat
     4921 atttcaaaga tacttttgaa aaagttcttg aagtatcaag tgaagacgaa atattaagtg
     4981 aaatacaaaa aataagaaat ggttttgaag ataagtatga catagtagaa atgttttatt
     5041 gtaaagatgg ttgtaataat ggagatggtt tgtaaatggt caataaattt aataataagt
     5101 ttttaaggct agtaaagagt gttctgatat tttttataat tcttttatta tggaaaatta
     5161 caaattatct tggtatttgg agtgattata tattaccatc acctgaaaag gtatatagta
     5221 catttttaaa tatgataagt gatggttcaa tatttattaa tgtatatgca agtatgaaaa
     5281 gagttttgat aggttttgca ataagtactg ctataggtgt gcctttaggg atattctttg
     5341 gaatttatag tggagtatac gaatacttta aatctctaat aaatttttta aggaatacac
     5401 ctccccttgc tttaatacca atgcttatat tatggtttgg tataggagag gaatcaaaaa
     5461 ttataattat agttttagca tcttttttcc caatctttac aagcacattg aaagggatta
     5521 aaaattgtga ctcaaaactg attgaagtgg gtagagtatt tgagttttca aagcttcaga
     5581 taatatttaa aataataata ccaaatgcca ttcttgatat tgctgttggg ttaaagttag
     5641 ctcttggata tagctttaga gcaattattg gggcagaact tgtagcagct tcatctggtc
     5701 ttggatactt gatatctgat ggaaaagaaa tgtctagaac agatgttgtt attgtaggga
     5761 taatcgtaat tggactttta ggaataatta cagattacat attttcgatt attgtgaaga
     5821 aggtaagtaa gggaaagatg gtggaagcat atgaataatt tagtatgtga ctctggatat
     5881 ataattagaa atttatataa aagttataaa gttgacaata aagaacatgt agttcttagt
     5941 gatatctctt taaacataga tagaaaccat ataacagtaa ttttaggtga aagtggttgt
     6001 ggaaagacta ccttgcttag agcaattgca tcacttgaag gtataaatag tggtgaaatt
     6061 aggtttatta atgataataa agagtataga cctaatgttg gttttgtttt tcaggagagt
     6121 agattaatgc cttggttaaa cgtaagtgaa aatatagctt ttcataatca aagaaaaaat
     6181 actattttaa acaaggtgct tagaaaattt aaacacaata aaagaattag gagtaaaatt
     6241 ataaatgata attttagaag cttagaaaca aatgataatt tgaacaaatc aaataaatat
     6301 aataggaaaa gtaatattga tgtagaaaaa tatcttaaga tgatgaactt gcagaaattt
     6361 aaaaactctt atccaaatga attgtcagga ggtatggcgc agagagtaag catagctaga
     6421 gctctttctt tcaatccaga tatgttgctt atggacgagc ctttttcggc attagattat
     6481 tttacaagga ttgatatgca aaatgaagtt ataagaatac ataagtcaac taataaaggc
     6541 gtgatatttg taactcatga tattgatgaa gcgttaaaga taggcaagaa aatagttgtt
     6601 ttcacagacg aaagaaaagt aaaagaattt aatatagaag atggttataa tagagattta
     6661 acttcagatt actatattaa attaaaaaaa gatattttaa tgacaatgaa gtcaaataaa
     6721 ggcatttaaa gaaattaata atattttaag gagtaaagta aatgaaatta aaaaagctat
     6781 tgataggtat aacgatatta acacttgcaa cagcatcttt agctggatgt gctaaaaaaa
     6841 ataatggaga aaaactttca gaaataaatt taacttatgt aaaatcacca ttaaatgttc
     6901 cttctataat tcagaaacag gatgatttat ttggaaagga atttaaaaag gataatataa
     6961 aagttaactt ccacgaaatt actacaggtc cagagcaaac acaagcttta gctgctggag
     7021 aaatagactt cttacatgca ctaggtggta catcagcttt aatagctgca tctaatggag
     7081 tagagttaaa gatattaaac acttatagca ggtcaccaaa aggatttatg attttgacta
     7141 ataataattc aataaaatca gcagcagatt tagtaggtaa gaaggtggca ggcccaaaag
     7201 ggactatatt acatcaggta ttgatttcag cacttgataa agaaggatta agtatggatg
     7261 atgtggaatt tgttaatatg ggtattccag aggcatcagc agctctttct gatggcagcg
     7321 tagatgctgc attgattgct gggccagcag ctcttaaagc tatgaagtca ggtagtaaac
     7381 ttgtagcaaa tggtgaaggt ttagtagatg gtataatagt aactgctgtt agcacagact
     7441 ttgcagagaa acatccagaa atagttgaaa gatttatgaa ggttgaaaag gaaactttag
     7501 aatatgtaaa taataatttt gatgaagcta tggaaaaagt agctaaagaa gttgatttaa
     7561 gtcttgaaga aactaaagaa ttatatgctt ggtatgattt tagtttagat ataacagata
     7621 aagacatatc ttcgcttgaa gatacacaag acttcttaat aaaaaataag ttacaagaga
     7681 aaaaagtgaa cataaaagaa ttaatctata atgctaagta gaaactatga taaaataata
     7741 ctaagtaaga aatataataa aagtttaagt taccttatta aggtaacttt ttctattttg
     7801 aattagtaat ttcaatttat tttagaatga ttagttgata ataatgatta attaatcata
     7861 aaaagatact tttttataga gttttattaa gatgtctaat aatatggtta tacttatgaa
     7921 gtcagtaatt gtgaagttag taatatgatt aaatttatat attgtaaaaa gattataata
     7981 tattaaaatt ggactgttat atgatgagta aataggtata ttaggaggaa ttatatgagt
     8041 tataaagtat atattgtgga agatgacata tcaataagta cattacttaa agattatatt
     8101 gagaaatatg gatttgaagt gattgttgaa gagaactttg atgatataat gattacattt
     8161 gaaagtttta agccacactt agttctatta gatgtaaatt tacctaaatt tgatggtttc
     8221 tactggtgta gatgtattag gcaacagtcg aatttaccta taatttttat atcagctaga
     8281 gatggtaatg tagaccaaat tagagcttta gaaagtggag cagatgatta tataacaaaa
     8341 cctttttact atgatgtagt aatggcaaaa ataaagagtc atataagaag ggtatatggt
     8401 gaatattcac ctaaaataga cgaaagaata gtggagctag atggattaaa attttatcca
     8461 gaaaggttag aactagaatt aaatggtgag aatctaatta taactaaaaa agaaggtata
     8521 ctgattgaat gtcttatcaa gagataccca aaagtagtaa gtagagatta tcttttagaa
     8581 aagatatggg atgatattga gtttgtagag gaaaatactt taaatgtaaa tgttagcagg
     8641 ataaggaaaa gatttgagga attaggtata gataatgctg ttcagactgt ccgagggtta
     8701 ggatataaat taaataagaa ttggtaagga gaaagtatga agttattttt aaaagattat
     8761 aaaggttata tttttatata ttatataggc atagttatga caattattta ctgtaatctt
     8821 atgaagttta ttagttttgg agagagttta tatattcttt tatttagttc ttttatattg
     8881 ttatgttttt taatatataa gtattataaa aatagagaag tatataatct gtttgaaaaa
     8941 ggagtacatt ctttggagga gtcatttttg tatttaggaa gctcattctt ggggagaaat
     9001 atatcagtta ttttaaaaca acaacataaa ttgtatcaat caatgataca agagcataaa
     9061 aagatacata gtgaacattt aactttcatc aaccaatgga ttcatcagat gaaaacacca
     9121 atatcagtta taagccttca gctccaagat tatgagggag aagaagtaac tgaaaatata
     9181 agaaaagaaa tagatagatt gaataaaggg ttagatatgg ctatgcattt tgcaagggca
     9241 gataattttc agaaagactt tatagttgaa aaagccaaat taaatgaaat tgtatctagt
     9301 attgtaaatg aagaaaaaag gttatttata aaaagtggaa tgattcctaa gattcaagta
     9361 gacaagttta cttatgttta tacagatgta aaatggatga aatttataat aggtcaatta
     9421 attacaaatg gagtgaaata ttctaaagat tatagtaact acctaactat aaaatctgaa
     9481 gaaaataata aatatgtaat tttaagtata atagatgaag gaattggaat tgtttctaaa
     9541 gatttaaaaa gggtatttgc cccatttttt acaggagaaa atggtcgtaa gtttggagaa
     9601 tctacaggta tgggtcttta tctagtaaaa aaggtatgtg atgatttagg tcataaagta
     9661 agtataaaat cagaagtagg aaaaggtaca gaagtttcaa tctactttaa taaaggtgta
     9721 aatagtttga taaagatgta aattatctgt tacaataggg tttttagtta attgtacttt
     9781 tttattatac aatttattaa atgaaatatt catagttaaa taatcaaaat ttaagagagt
     9841 ttatttgcaa accttacata gatgtaaggt ttgtgtaatg tttgcagata gttaaatttt
     9901 ctaatctaaa ttacaattaa tataagaaat aacataagag aaataacata aaaaaagaaa
     9961 ttttgtaaaa aaggaaggat gtttattgat gagtttagat gttattttta tattgaaatc
    10021 agtcataata gctatagtag aggggcttac agaatttatt ccagtttcat ctacaggtca
    10081 tatgattttg gttggtaatt taatagattt taaggggcaa tttgctgaaa tgtttgaggt
    10141 tgtaatacaa cttggggcta tattagcagt agtagtactt tattggaaaa agataaaaga
    10201 tagcgttata gaatttttta aatttatatt tactggtgga aaagaaggaa agattggttt
    10261 tagatttgga atgaatgtaa ttataggttg tataccattt gcgattatag gagttctatt
    10321 ttacgataat ataaaatcat tgtttaattt acaatctgta attataggat ttatagtggg
    10381 tgggatttta ttattagtag tagaaacttt gtttagaaaa aagaatcatt ccacagataa
    10441 tatagataaa attactccaa tacaagcttt aaaggttggg acattacaag ttttgtctgc
    10501 atggccaggt atgtcaagga gtgcatctac tattatgggt ggatggatag caggacttaa
    10561 ttctcctaca gcagctgagt tttctttctt tttagcagta ccagctatgg tggcttcttc
    10621 aggaaaagat ttatttgaat ttgattattc aataatgaca ccaacattgt ggattgcttt
    10681 ggtagttggg tttatagtag cttttattgt atcaataata gttatggaaa aattcgtgaa
    10741 ttttttaaag aaaaaaccaa tgagagtgtt tgctgtatac agaataatca tgggtgtagt
    10801 acttgcagta ttagctttta ctaacataat tagtgtatag aaaaaataga tttattatct
    10861 ataaaaatta aactaagata aattataatt aacaattata tatatgttat ttcaaaaatt
    10921 aaattaggaa ggatagttaa ataatgaaaa ttttaactgt aaataattta agtaaagtat
    10981 atggtaaaaa aataattttc aacgcattaa atgacataaa ttttagtata gaagatggag
    11041 aatttgtagg aattatgggt ccatctggta gtggaaaaac tactctttta aatatgattt
    11101 ctactattga caagccaact acaggaacta tggaattaaa gggcaagaat cctttattgt
    11161 taagaggtga ggaacttgca ttatttagaa gaagagagtt aggttttgta tttcaggatt
    11221 ttaatttatt ggatacatta actataggag aaaatatagt attgccattg acattagata
    11281 aggtcagtgt aaaagagcaa gatgagagat tgaatgaagt atctacaata ttaggtataa
    11341 aagatttatt aggaaaaaga acttttgaag tatcaggtgg tcaagctcag aggactgcta
    11401 ttgctagagc attgattaac aatccatcaa tacttttagc agatgaacca acaggtaatt
    11461 tagattcaaa gtcatcaaag gttgtaatgg agctatttca aaagataaat aaagaaaata
    11521 aagttactac tatgatggta actcatgacc cattagcagc gagctactgt agtagaatct
    11581 tattcataaa agatgggtct atatataatg aaatatataa gggtagtagt agagagcagt
    11641 tttatcaaga gataatggat gtacttacat tattgggagg cgataactaa tgaatataaa
    11701 acagtttgct attaacaata tttcaagaaa tattaaagcg tatttaggat acttagcaag
    11761 tattgttata tcatcttcac tgctattttc ttttattatg tttacaagtc atccagattt
    11821 agatgtatct caatttcctg attatttaaa agaaggtctc aagatgagta agataattgc
    11881 atatctattc ttattcttct ttgtgtttta ttcagttagt gtgtttctta aaagtagata
    11941 taaagaattt ggaatacttt atattacagg tatatcaaag cgccaagtaa tgaaattaat
    12001 ctttatagaa aatatgataa taaatatagt atcttctgta attggaataa taataggctt
    12061 aatctttgca aagatatttt tagtggttat gtctactttt ttagaattat caccattaaa
    12121 attctatata ccatttaatg cgatattgtc tactttaatc tattttatga ttttagctgt
    12181 attaacatca atatttactt cttttatagt taaagaaaat caggtgttga agttacttaa
    12241 aggtacaaaa actccaaaac cagagcctaa aacatcacta atgcttgcta tattaagtat
    12301 ttgtttgttt ataatagcgt attataatgc tattacctca accgaccaat atacaacata
    12361 tctgcgctta gttccagtta catcccttac tatagtagca acttacttgt tcttttctca
    12421 atttagtgta ttttttatga agaagctaaa attaaacaag agattttata gaaaaaatac
    12481 aaatatgctt tggatttcga acttgattta taaggtaaag gataatgcta gaatattttt
    12541 cttgataact ataacttctg cagtggcatt tactgctata ggtacagttt attccttttg
    12601 gaaagatgta gagcgtcaaa ttaatttaat atatcctaac acaatatatt attcaacaat
    12661 gacattgcat aatgacccta aaaagcctga tagcaaagaa aaagacaagg aaagaatgat
    12721 gtttatagaa aataatctaa aaaaagagaa tgtagactat acaaggatta atggtgaatt
    12781 taaaactgta tttcccaaga aaagtgattt taatgtcaga attatgaaag aatctaagta
    12841 cttagaaatt acagaaacta taggggttaa gcctataagt tttgaagata atgaatgtat
    12901 atcattatta tcatcaagtt taccaggaaa taagaacgtc aaagaaaata ttattgtagg
    12961 gaatagaggt ttaaaggtgg caaaacaagt ggaagaatgt gttatgccag cgtattacaa
    13021 aaatatgtat gtggtaaagg ataattttta tgatagtata gaatcaaaat ttatagtgga
    13081 tagatttact gcatttgaag tagaggattc aagtaaaact atagagatat gcagagaatt
    13141 tgaaaataaa tttaataatg agtcagggat gcaaccatat ttgtttttct ctaaagaatt
    13201 aatgatagga actgggaaat taatgtattc tacatttata tttttagcaa tatttatagg
    13261 tttaattttc tttgttacta caagtagttt tttatataat aaattttata tggattgcca
    13321 agttgataag aaaaagtatg aacagttaaa taaattgggt atgacatata atgagattaa
    13381 aaaagcttca acaattgaga ttggaatagt atttttgttt ccttatgtag tggctgttat
    13441 acattcaata tttgcactta ctgcactgaa aaattctttt gatatagaag tagcatcatc
    13501 agctgttttg gtaatgggaa gcttgttctt ggttcaaata gtttattttt taattgtaag
    13561 aaataattat ttaaaagaga taaggttaaa tttagtcaat ttttagttac tattattaaa
    13621 tgataataaa aagggtttag aattaattaa tattactgga attatagatg tttatttttt
    13681 cagtaatatt ttttattgta ttaactatta aaattttgta caaatataaa atataatatg
    13741 gtaaatgtca aaattataaa aaaattctac ttatataaaa tataaagagg ttatatgtac
    13801 atatgtgtag aaataaataa taaaaatatc aattaatgtt attagggggg gttactcatt
    13861 agtaaattag ctaatacact tagaatgatg atattaattc aaagcagagg caaaatgaaa
    13921 tgcaaagaat tatcagaaga gttggaagta agtgaaaggc aaataaaaag ttacaagatg
    13981 tatttagagc aagctggtat atttataaat tcaacacctg ggatttatgg tggttatgaa
    14041 atagataaat gtaatagcat ctcattaatt aaattattag atagtgaagt ttcaattcta
    14101 gatatgataa attcacagtt agagtataat aatgatattt acaaaaatga atttaataat
    14161 attgtagaaa aaataaaagc agtattaaat acaggagaaa aaagcgatac atatatggat
    14221 tattttacag ttcaagcaca acgtaattgt gattatgagt ctgaaaaaaa taagtgtaat
    14281 gaaataatta gagcatatac cacaaaacac aaattttgga tagaatatta ttcattaaat
    14341 tcaggaaata gtgaaagaat tgttcatcca tatggtttat ttaattataa atcagatact
    14401 tatatggtag ctttttgtga aaaaagattt aagtttatag actttaaatt atgtaggatt
    14461 aaagattata aggttttaga agaaaaatat aatgtagata aaagttttag ttgggatgaa
    14521 tatagtaaaa atagtatagg aatttataag ggagaagaaa ttaatgttgt tataaaaatt
    14581 agtcatccat tttcaacaat tataaaggag aaagtttggg ttaataatca acaaatcata
    14641 gaatatgatg ataaatctat aatgtttaaa gctaaaatga gaggttatga ggagataaaa
    14701 agttggattt taagtatggg agcttatgtt gaggttgtag aaccagatag acttagaaat
    14761 gatatattgt tagagattga aaaaatgaaa aaaatttact gattttttca aataatttat
    14821 acttggacca atatacttca cggtctagtg ttaatatatt aattgagaga ggtgatatta
    14881 tgaaaatgaa aattgaattt agtacaggtt gtataccaat atcttataat tcgctcttta
    14941 tgagtattat aaaagaagca ataagaaaat ctaatgaaga ttactataaa aacatgtact
    15001 attataaaga aaagaataat aaaaaaacta aaaattttac attttcggtt tatataaaaa
    15061 agtatagtat tgaaggtgac aattttatta tagaagataa agtaatatta aatataagta
    15121 ctccagattt agaattgggt tttcatatat acaatgggtt aatgacttct aaaaaatgtt
    15181 tatataaaga ctacgaactt acaagaatta gaatcgattt atcaagagaa aagaaggtaa
    15241 cagaagaaag ggtactattt aatgcattgt ctccaatctg tgtaaagtct aaagaaggaa
    15301 aattcttgga gataactgat gatagataca tagaagaact aaactatata actaatgaag
    15361 tggttaaaaa ttataggggg aatggattaa aaagaaagtt agaatttgaa aatataggtc
    15421 tcaaaaaggt agtagttaaa gagagtttaa gagaatttaa aaaaataaca ggtaaagagt
    15481 atcaatatat taatggttac aaaggtaaat ttatattgaa aggtgatatt gatgatttaa
    15541 atcttatcta caatctagga attggtttta gacgtgctca gggatttgga gatgtagata
    15601 ttttagaatg gaggtgagat aaatgaagtt aagagtttat agaggagatt ggttttttaa
    15661 tatgggtata gttggatttt taaatataat aaagaaagca gataaacaag ctgaaatatt
    15721 tattatggaa gattatatcg aatttgatag cttatttctt gaaaactttc atgagtatta
    15781 ttttaattac tttatggatg aatatgatgt atcaaaaaga attaaaaaaa atattgatta
    15841 cagtataaat tttattaaat ctaagcctga tagaatcaaa gatggaatta agaaaattaa
    15901 agatagtgtg aaacaacaaa atgataagat taaaaaattt gatgaagaaa aatttaacat
    15961 gataaaagag aagctagata gtatgtctaa gataaaaagt tatgatgaat ttgatagttt
    16021 agaagcttta gtagatgaga ctatagatat atttaaaata aaatcaataa atgacaggct
    16081 tacagcaaat ctttataaat atgtagttca agataattat tttggacaag ttagtttttt
    16141 taatgttgct aaggcgaaat tagatttaga tggattaaaa caggtaatgt ttaatgatta
    16201 tttaagacaa attatatatt ttggagagtt agaagattta ttagaagaga atgactatga
    16261 caagttgaaa aactatttaa atgatagatt aaatagtata gctaaggata taaatgaaaa
    16321 aaaagttagt aagtctagta taaatactat agaaaaaatt atgaaggaaa taaataaaaa
    16381 ctttataaag aaaaataaaa gcatagaaga tataaaaaag tatatggata gtttagaagt
    16441 atgtgaaatg tgtggtttat ataagggaat attagatgaa tactcagaaa gtaattttgc
    16501 tccacttgga gtaagcacca ataatgctag gaatatgttt tggaatcagg cgtatgtacc
    16561 atcaatatgt gatatttgta agctaatatt gttctgtact ccagcagggg ctacttatac
    16621 aagaaagaat tatttaatta atgaagaaaa tgagttttat ttatttgtta acatggatac
    16681 ttgtataaat gagctttttg aaagaaataa tagtttaaaa gctcaaaaaa gtgagtattc
    16741 agattccaaa gatgaaaatc cttttaatca gcttataaaa agtatagtag aagaaaatac
    16801 attaaaaagt gaatggcaac ttcgtaatat actttttgtg gaatttaaag caagtataga
    16861 ttctaaaaaa tgtaaaataa attattttaa tattccaact tatttagcaa agttttttgt
    16921 aaaagaaaat aaaaaaatac aaagtatata tgattataaa cttaaatcaa atgtgttaga
    16981 tttaatttta aaaagtaaag acttaaaaca tttaattaat aacactttaa ggaatatagt
    17041 taagagtaat ttagaaagca ataataaatc aaacatatca gggttatctt gtttcaatgt
    17101 tgttcaatta agaacaatat taaataacta taagagagga gtgtataaaa tggactataa
    17161 aaagttgaga aagatttatg agtcaggttg tgaaatacat gattattata ttgataagaa
    17221 tgctaaaaat aaaatagaag gaataacata taaaatttta aatttaatta aggttggtaa
    17281 taaaaatgat tttatgcaaa caattttaag aattttttca ccaagtgaaa aaacaccacc
    17341 agaagaaatt ttacagatat ttgttgaaga agatttagat tttgaatctg tagggtatgc
    17401 atttgtaaca ggattaattt ctggaaaata tgaaggaaaa gataaactac caaacgaaaa
    17461 ggaggaaaaa taagatgaaa agagggttaa catttacagt aatagcacag gctcaaagtt
    17521 taaactatgg ggaagggata ggtaatatat cagaattaaa aaaattaagt agagatgatg
    17581 gaaatatata tacatttgca tctcgccagt gtttaagata tgacatagta cgattagcag
    17641 cagaattgtt taattggaat ttacaggttg tcgataaaga aaaaggcact attcaattta
    17701 aagacaatat aagtataaga gaatcagaag aaatggattt atttggatat atgaaaacaa
    17761 ataaaaaaga tgagaaagat aaaaaaggag gttctttgac tagagaagct actgtaagat
    17821 taagtaatgc aatttccctt gagccatata gaagtgacat ggacttttta aataataaag
    17881 gattagcaga tagaatagga gaacatccta atcttgctaa catagagcaa catttaagtt
    17941 attatacata tacagtgact atagatttat ctaaaatagg taaggatgga gatatagaac
    18001 ttgataataa agagaaatgt agaagagtag tagaattttt agaaataata aaagtattga
    18061 atagaaatat tagaggtaga caagaaaatt tatctcctct atttgtagta ggaggagtat
    18121 atgaaatagc aaatccattt ttcttgggta gaattaaatt aaaaggagat aaaaatggat
    18181 ttaaaataaa taagcaagct atagaagaag taattcaagg tactttttta ggaaaagatt
    18241 taaaagaatt tacctatgtt ggaatggtag atggggtatt tataaataaa gaagaattta
    18301 agggattgtt tgaagataat tttttaagtg tagataaatt ttttggacaa ctagttaaag
    18361 aagtaaagga atattatggg gtgaattaat atgaaggttt tgaaattaga tttatttcaa
    18421 gaaactgcat gctacaaaaa accatttgct atgaagatta cagagacata tccactgcct
    18481 ccatattcta caataaatgg attaattcac aaaatattaa atgctacaga atatatacca
    18541 tttaatataa gtgtacaggg aacatatgaa agtatattta ataattacca gacaacatat
    18601 ttttataaaa aagatagtat tacatctatg ccaatgaata gtcatatgct attgaatgta
    18661 aatttgataa ttcatatagg tggggatttt gatttattgg aagaaatata tgatagttta
    18721 ttaaattatg acgagcactt atcattaggt agaaaagaag atttagtaag aattaatgat
    18781 attagatttg tagaagtcaa taaatttgaa gtagatgagg atatacaatt agatgattat
    18841 gaaaattata agttatcaga atgtaatata agaatgccta tatatatacc aaaaacaagt
    18901 ttacaagata cagatataga tggtataagt tacagactca ataattacta tgataataat
    18961 gctgtggaag ataaaaaaag ggtttggaat aaggtagata gttattatgt tgaggatgga
    19021 aatactatat caattggaga tatattatta gataatgaag gagatttatt atattttaat
    19081 aatataaatt aggagggaga ttatgttata tgctaaatct aatccagtgg aaacacttag
    19141 agaacatact gatgaacttt taaaacaaat gaatgtatta agagaaagtt atggaaaaaa
    19201 tataaatagc ttagattttc tagaagagga gatattttgg caattacttg actttgttat
    19261 agaatttcat gatattggaa aagcattttc accgtttcaa gagttaataa aaagcagaat
    19321 ggatactaaa ataaatgaac ctaaaatagt aacttattta gaaaataatg taggccataa
    19381 ttatctttct cctgcattta ttgattatag ttatatagac aggaaaaaaa ataaagaact
    19441 tagagcagta ttaaatcaag ttattgtata tcatcatgaa agagatatct ttatagataa
    19501 agattttaaa aatttaatac aaaaaatatt agatgaagat ttaataaata aagtttatga
    19561 actacagcat gaatttaaag ttagatatcc aataaaaaca gaaaaattga gtaaagtata
    19621 tctacaaagt gtagaaaaaa gaatagataa aaatcacaag tactataatt tatatattat
    19681 gttaaaagga atactacata gactagacca tagtgcttct gctcatgaag ttgtggaatg
    19741 caataatgtt ataaatatag gagaacagac agaaaattat ttaatgaaag agtttggtag
    19801 tttgagagag gctcaatcat ttgctaaatc taatagaaat aagaatataa tactgattgc
    19861 atctacagga atgggaaaaa cagaaactgc cttaatttgg atagataaag acaaggcatt
    19921 ttttacactt ccattaagag taagtataaa tgcattattt gatagagcaa aaaatatagt
    19981 tggtgttgga gaatcaaatg atacattctt aggtttgtta cattcaacag caatagatta
    20041 tctagaagaa agtaatcaag aaaattcaag tgagattgta gatttagcaa agttattatc
    20101 ttgcaaactg acattttcta caatagacca aatatttaaa tttccttttt tgtatagagg
    20161 gtatgaaaaa gtgtactcaa cattagcata ttcaaaagta gtaatagatg agatacaagc
    20221 atattcacca gaaatagcag ctgtattggt aaaaggtata gaaatgatac ataagatagg
    20281 tggcagattt atgataatga cagctactat gccaaccata tatatagatg aattaaaaaa
    20341 aagaggtgta atgaatagta acttagctga attgacatgt aatacagaaa aaataagaca
    20401 ttgtgtttct attgtagaaa attcaataga tgaaaactta ggtaaaataa tacaaagtgg
    20461 aatgaataaa aaagttcttg taatagtaaa tacagttaaa agtgctgtag aaaaatatga
    20521 gttaatagaa gaaatagtta aaccaaaagg aatagatgtc aacttaaatc ttttacattc
    20581 tatgtatata caagaagata gagcaaaatt agaaaaatat ataaaagaat ttgccaatag
    20641 tgatagaaat ggaatttgga ttacaacaca attagtagag gcatctcttg atattgattt
    20701 tgatgagctt catacagaaa attcaacact tgacagtcta tttcaacgat ttggtagatg
    20761 ttatagaagt cgtgagtatg aagaaaatag cccaaatata tttatatata cagaaaaagc
    20821 tacaggaata ggtagtattt atgataagga tattgtagaa aaaggacttg agctactgaa
    20881 aacttttata aatggaaaag aatgtgtgaa gatgaaagaa aaatataagg tagaaatggt
    20941 taagatacta tactctaaag aaagtctaga aggtacagct tttgaaaaga gatttaccag
    21001 tgctttaaat attttagata ctataacgcc atatagttta ggttctaaag atgcacaaaa
    21061 tatacttaga aatattgaag gatatacagt aattccagag gatatatata ataaaattga
    21121 agaaacttta atcaaagatt atgaagagtt gggacaagag cttataaatg catatagtaa
    21181 tgatgataga caattgataa ataaaataaa gtctaagaga aaaaaagcaa gaagagagat
    21241 aattaaaaaa actgtaaatt taccaatata taaagctaaa caaaatgtaa ttgacataca
    21301 ggtgaaaggt cttgaagatt taaaaatact tttatataaa tatgatatat acgaaaataa
    21361 gcatacaaaa cagatatttg gtaaaggtgt gcttataagt aatgaaatag accaatttat
    21421 ttgattttta tattaacaat gtggctaaaa tttactaaag aaggtgattt tgtgccaata
    21481 gatttagaag tattaaaagt tcaaggtgta aaaataaatt attattatgt atgtaaaaga
    21541 aaactatggc tattttctaa aggaataacc atggaacaaa atagtagtag agtattgtct
    21601 gggaagatag tacatgaaga ttcatataaa agaatgaaaa ataaagaaat attgattgat
    21661 gatatcttga agctagatat aataaaggat gagtatgtaa aagaagtaaa aataagttca
    21721 aagatgaaag aagcagatga aatgcagtta tactattact tatactattt aaagaatagt
    21781 atgggtatag agaaaaaagg tactataaat tatgttaagg aaaagaaaca agaagaattg
    21841 atattgacag aagaaaaaga aagaattatt gaaaacacac ttataaatat aaatgaaata
    21901 aatatgcagt tatctcctcc caaaattgag aaaaaacatt attgtacaaa atgtgcttat
    21961 tatgaatttt gttttgtgaa ggaggagtaa caatgtataa agactattat attataagta
    22021 atggggatat aaaaaggaaa gaaaatacga tttactatta caatttagaa ggtgagaaaa
    22081 aaatattacc agtagaacaa gttgatgata ttcatatata tggagaagta gatttaaata
    22141 caaaacttat aaattacata agcaaatatg gggtgatgct tcatttctac aattactatg
    22201 gatattattc aggtacatat tatccaagga agaaaaatgt atcaggattc agtttagtat
    22261 gtcaatcagc ctgctattta gataaaaatg aaagattata cttagcaaaa tcctttgtgg
    22321 aaagtgcatc ataccatatt ttaagaaata tgagatatta caaagttgat gaaagtttaa
    22381 ttgatactat aaaggatgaa ctaaatgatt tgaatgatgt caaagaaata tcagaactta
    22441 tgggtaaaga aggcagaata agaaaaacat actataaatc atttaatcaa atattaaaag
    22501 atgattttga attttataaa agagaaaaaa gacctcccaa agacccagta aatgctctta
    22561 tgtcttttgg aaatagcata atgtatacga atgttttaag tgagatatat aagacacaac
    22621 ttgaccctac aataagcttt ttacatgaac catcaacaaa gagattttct ttaagtttag
    22681 atattgcaga aatttttaag ccaattataa ttgacacaat aatttttagc ctgattaata
    22741 ataaaagaat taataagaat gattttgaat atcaagacaa aatttgttat ttaagtgaaa
    22801 aaggaaagaa gaaatttttg caagaatttg aaaataagat gcagacaact ataaaacata
    22861 gaaatctaaa tagaaaagtt tcatatagaa tgtttataag gttagagtgt tataaattaa
    22921 ttaaacattt tataaatgat gaaaggtaca aagttttaaa ggtgtggtgg taagtatgtt
    22981 tgttattgtt acttatgata ttgttgaagc aaggtcgtta aatagaatta gaaggatact
    23041 tagaaaatat ttgacttgga cgcaaaattc tgtttttgaa ggcaatatta ctgaaggaaa
    23101 gttacataaa tgtatttctg aaatagaaaa tattattgat aatagcgagg attcaatcta
    23161 tgtttatgag ataaaaaatc ctaattcaat taaaaagaaa tgttatggga ttgataagta
    23221 ttctgatgaa atgtttatat aggtttgcag tgagcgatat ttatgctaaa ataggtgtta
    23281 acagttggaa tataagggat tgaaggtgta tgataattgt tatcaattgc actactgctc
    23341 gctcactgca aattttgatg tttttattga attataattg cttgattgaa gtgttttcaa
    23401 tgtattcaaa tatacctatt ttggggtttt atattaacta agtggtatgt aaataatttt
    23461 ttcggatatt taataattta ttttaaattt agttttatat taactaagtg gtatgtaaat
    23521 ctcaacagta tctacaagaa cttctatatc ttctaagttt tatattaact aagtggtatg
    23581 taaattaatt tagggtaact tttatattat tttcttctat cgttttatat taactaagtg
    23641 gtatgtaaat ttggttgata tagatattag tcctattatt tctgcttgtt ttatattaac
    23701 taagtggtat gtaaataaat tagataattt ataagtcaat aaaataagtc caagttttat
    23761 attaactaag tggtatgtaa ataaatttgt aatattatat aatcatccta aaacaaaagg
    23821 ttttatatta actaagtggt atgtaaattc gaatgctata attttaatat ataaaataaa
    23881 tggagtttta tattaactaa gtggtatgta aatatggatt ttagtcgttt ttaaattttt
    23941 ctcgtaatag ttttatatta actaagtggt atgtaaatac cattcattta tatttttatc
    24001 tgtatctctt agccatgttt tatattaact aagtggtatg taaattaagt tttcttaaaa
    24061 aagtacttgc tttaaatatg ttttatatta actaagtggt atgtaaattt tattttctct
    24121 ttattagtca ttagtttttg ccttcgtttt atattaacta agtggtatgt aaatatgaag
    24181 aatttgcgaa actaataaga aaagatattg agttttatat taactaagtg gtatgtaaat
    24241 ctttctttat caaagtaagc taaatttagt tcctccgttt tatattaact aagtggtatg
    24301 taaatcgtca ttacttttta ttgcttccat taaatttgtt cgttttatat taactaagtg
    24361 gtatgtaaat atcgcaagat aggctaaatc tatcaaggag agttgagttt tatattaact
    24421 aagtggtatg taaatgactc tgccatccct gtaacaaaca gtcttaccgc aagttttata
    24481 ttaactaagt ggtatgtaaa taaatatttt acgattagtt gatgctataa ttctattagt
    24541 tttatattaa ctaagtggta tgtaaattct tttgatttag cgttgtttgt ccggaatttt
    24601 aaagttttat attaactaag tggtatgtaa attcgggcta gaatgtattt taaaccctag
    24661 ttcattgaag ttttatatta actaagtggt atgtaaattt tctaatatcg ctatttaaac
    24721 tcaatgcttc atttgtttta tattaactaa gtggtatgta aattcagaac aaattaaata
    24781 tgtttcgttt tccatgtaag gttttatatt aactaagtgg tatgtaaata gaactttttt
    24841 atattagaag tacttgacat gattgccgtt ttatattaac taagtggtat gtaaatcttg
    24901 ataatatttc agcatatgat actgaagatg gttttatatt aactaagtgg tatgtaaatt
    24961 tgccaatgta acatctttag atattacagt atcattgttt tatattaact aagtggtaga
    25021 taagtagaat attagcaata tcaacgagta tttagaaact tattttatat taataaagca
    25081 gtatataaaa ttgtagaatc aacaatagca tatactaaaa catccacctt atattaacta
    25141 aatcatatct aaataaaata aacttaccta taaacattat aaaatcaata caaaaatgag
    25201 gtgaaataaa atttatgata aagaaattaa acaataaaga tataaataaa atcatggaaa
    25261 tatgggaaaa aagtacaatc aaagcacatg actttataag taaagaatac tggcaaaata
    25321 actataatac tgttaaaaac gaatatatac ctatatcaga tacatttgta tatgatgatg
    25381 gagatgaaat aaaaggattt ataagcataa tagataaaag ctttatagga gctttattta
    25441 taaagcccaa ataccaaaat ctaggtatcg gaggtaaact tttagattat gcaactaaaa
    25501 aatataaaag tctaagctta gcagtatata aagataataa aaaagcagtt gtgttttata
    25561 ataaaaaagg ttttaatata gtaaaagaac aagtaaatga agattcagga tttaaagagt
    25621 acataatgga atatagtaaa taatatgatt acattctatt aaaaggataa ttaatttatt
    25681 tgccatttga ttttttaaga gtaattttta aaatagacta tatgaagata aagatattta
    25741 ttatagatgt aagttaatca gaattgatat agctacaaaa gaggaaaata aaattgtatg
    25801 gtgtatttca gatttaacaa atttactatt taaagataat aagtttgata taatattaaa
    25861 gatactttca cctataaact atagggtatt tgaaagaatt ttaaataatt atggaatgct
    25921 aataaaggtt tgttaaaatt taataattat tagattatag aaaattgcca tgaaattagt
    25981 tcatcaagaa ctagtttcat agcaatttta attcttataa gtgtatttat ttttacagat
    26041 ttaaaaaact tatacagtat ctaataatgt aatcttatta ctttagccta taaatattta
    26101 aatcttaaaa tgttctcttt gagataagtt tatttcagag tcacaatttc cacaacagta
    26161 agatgttgag acatctttaa atttttcagt attctcatca aagttcattt ttttctttgc
    26221 aataattcct accttagtta aattagaacc acaattatta cagttttgaa ttttgcatag
    26281 gttatatagt ttatcaagtg taatatcttc taataattca tcacaattac aacatctgat
    26341 agaagaaact tcactacaca tttcaatagg ttcagcttct ttagaagaaa atttatatgt
    26401 tatgtcattt gatactttat aacctgtatc aaatatatta gaattacaac ttggacataa
    26461 gtactttgga agatatatat cttttttttc aaaagtatca ctactaatat tacagtttaa
    26521 ttcatctaaa attggtcttt catctaggtt attaccttta tttaagctta gaagataatt
    26581 ataaattttt ttatcttcat tctgttttga tgtatctaac aaatgctcca ttatattagt
    26641 gtattttttg ttttttgaag gcatgtttaa atccagataa ttgttttcaa tagcaaagca
    26701 tactatattg aaatagttat atttaactgc aaaaaataat aacatttcat caaatacttt
    26761 atctattatt ttgtcagaaa tacttatcca aaaatctggt gactctaagt aaactttcct
    26821 actaagttca tctaactctt catctatatt atttatatta ttttcattaa ctttcatatc
    26881 atcacttcct tttaaatatc ttgtgtataa actatttgtt actatttttt atataaaata
    26941 aaatcttata taaaaatata tcatattata atttacaagt atagtctaaa tagagctgtt
    27001 tttaagcaaa gttttataaa tgactattaa aatataaagt aacagtataa agtttactta
    27061 taaattaaat gtaaacctaa atgcttataa tagttgacaa aaaaacgtct ttattttata
    27121 ctaaaacaaa gctttaatag aagctttgta tatgtacata taaactaaat gaaataagac
    27181 atatgaaggg aattataaaa atgaaaaata ctaaagtaaa tattcaagat ttaactaaaa
    27241 tgtcaatatg tgtagcatta ttgtgtatat catcttacat atatatacca ttgccattta
    27301 ctccagcagg agtaacagca caaacaataa tgataaattt aatagctttg attttaacac
    27361 caagacaagc atttagtaca gttggtgtat atataatgat aggtttaatt gggattccag
    27421 tatttggtgg aggtacatct ggtattggaa agttattaag cccaacaggt ggtttttatt
    27481 ttgggttttt gtttgcagca ctaattatta gtttattaaa aggacgaaat aatgatatta
    27541 agagatatat actaataaca atttttgttg gaatgcctat tatatatttt atgggaacag
    27601 tatttatgtg ctattttaat caaatgacag tagaagcagc actttttgca gcagtagtac
    27661 catttttaat tggagataca ataaagtcag ttattgcatc ttttttaggc actaaactga
    27721 acaatgtact cagaagaaat ctatgataaa tctataatat aaggaagggt gtaatgtatg
    27781 aaggaagttt ttatattagg aggattgaga agtcatatag gtctaaaaaa tggaattttt
    27841 caatttgttc aacctgaatt actaggagca agtgttttaa aagatttaat taaaaagtat
    27901 gaaattgata aaattgatga gattatatgt ggaaatgcag ttgggacagg agggaatata
    27961 gctagattaa tgactttaac agcaggagtg tcaaatgaag tacctgcttt tactgtggat
    28021 atgcagtgtg cttctgctat gatgagtata gacatagctt tttcaaaggt taaatctggt
    28081 cagtgtgatt taattattgc aggtggtttt gaaagtagtt ctctacaacc aatgcgcaca
    28141 tatcataaga atgataagcg atataataca aataatccaa attatacagt tgctcaattt
    28201 tctcctgatg ataatagcca aaatagtatg ctcgaaggtg cggaaagagt agcagagtta
    28261 tatcaaattg agaaagcaga cttggatttt tgggtaaagg aaagtcataa aagagcaaaa
    28321 gaagcaagag aggaaaaaat acttgaagat attatatctc ctataaataa tagtacctat
    28381 gatgaaggta tacgtgataa gatgagtcaa aggttattag ataggatgcc atcaatttta
    28441 ggaaaagaaa ctattacaaa tgcagcaaat tcatgtctta ttaatgatgg agcttcattt
    28501 attataatat gttctaagaa gtatttagaa catgtaaaaa agaagcctaa agcaaaaatt
    28561 attaatacat gcacaatagg aacagatgca accttaagtc caacatcagc gatacaagca
    28621 atggataaac ttttagagat agaaagtctt aattatttag atgtatctgc tgttgaattt
    28681 aatgaagcat ttgcagttat agatgtgttg tttcaaagaa aatatccaga gttaatagat
    28741 agatataata tatttggtgg agctttagcg tatggacatc catatggtgc ttcaggtgca
    28801 attattgcat tacatctttt aaaagcatta gaaaagacta agggtagata tggaatttgc
    28861 tctatagctg cagctggtgg tctaggttca gctttactgc ttgaaagggt gtagattatg
    28921 aactattatg aacttttaag aataaaaaaa gagaggcata aaaataaaaa ttttttaatt
    28981 atagatggag aaaaatatac ttatgaaaat atattgagtt atagtgagga actaggaaaa
    29041 caaatttctt taggtgaaaa tatcccaatt ttaatatatt ctaaaaatat aatgtttcag
    29101 ttgattagtt tttttgctat taattattca aaaaatattc ctattatttg tcattataat
    29161 ctttctaaaa aagtattaga tgatatttta ttaaaaaata atatttcaat cattatatct
    29221 gatgaatcta tggacgttat tgatttatat gatgataaca ataatgaagt agcaaaaaaa
    29281 gttaattttt ctatttttaa tcctaaattt aatatatata tgtatcaata taagaacctt
    29341 ataaattatt tagataaaag tatttgtatg ggagctttat cttcaggttc tacaagtgtt
    29401 ccaaaggtat tatatagaac atatgagagt tgggctggat tttttcctat acaaaatggt
    29461 atttttaata tctctgggga atcaatcttg tttgttaatg gaacacttag ttttacaggg
    29521 aatctaaatt ctataatgtc agtattatat gaaggaggta gtattgtaat aagttcaggt
    29581 cttaattgta aatcatggat aaggataata aaacaatata atgtaactaa catatacctt
    29641 atacctacaa aacttcaact tttggttaaa cacttaaaag aaccagtact caaagttgta
    29701 agtattttta cagggtctca attattattt gaagatactg caagaaatct aaaaaaatat
    29761 atgccaaagt ctgaaattat tttatactat ggggcaagtg aattaaatta tattacatat
    29821 ttatgctatg atgaactaat tgaaaaacct ctaagtgtag gcagaccatt tccaggaata
    29881 gatatatata ttagagaagg aaaaattttt gtgaatacag aatatgcagt atataatgca
    29941 accaaacctt attctgtcaa tgatattgga tattatgata atgatggata cttaatattt
    30001 gaaggaagaa gtgatgacat tttaaatatt ggaggattta aagttagttc tactaagatt
    30061 gaaaatgaag ttaaaaaaat tcctcaaatt gaagatgcaa ttgtattgcc gtactcagac
    30121 actataagag gaaatcaaat tgtattattt gtaataacta tagacaaaat tacgaagaaa
    30181 gatttgttga ttaaaatgaa agataatcta atgaaaaatg aaatcccaaa gaaaattatc
    30241 tttttaaatt catttccata tacctcttct gaaaagatag atagattagc attattaaaa
    30301 atgttataag tataatattt agtaatttta gtgaaccaat gtattgataa aaacatattg
    30361 tatatagtaa aggaggctta caatatgtca aatcaatacg atataactgt tattggggga
    30421 gacctgcgac aagtgtacat ggtaaaaaaa ctaattaata aagggtattc tgtagtcact
    30481 tatgggatat caaatgaaat tataaatgga atcgttggaa aatcaacttc actagctgaa
    30541 gcactatcca aaagttccat tattttatgt ccaattccat ttacaaagaa tcaagttaat
    30601 attgaaaata cggatgattg tcttgatgct actattgata acttgctaat taatttatct
    30661 cctaatcaaa tattatttgg aggtaacata ccaactaaag tttgtaaatt ttgcgagcaa
    30721 cactctattc agatttatga ttttatgaaa atgaatgatg ttgctatttt aaatgcagtt
    30781 gctacagctg aaggggctat agcagaagct attaaaagaa gtataatcaa tatacatcaa
    30841 agtaactgtt tagtattagg ttatggaaga tgtgcacaaa tacttgcaaa aaaattatac
    30901 gctttggatg caaatgtatg tgttggcgca agaaataatg atgcaagaag tacggctaat
    30961 gcatttggat attccagtat ttctttaagt caacttgatg aaaaactttt acaatttgat
    31021 tatattttta acaccatacc agagtgtatt ttaacgaaag aaaggttaga aaaagtatca
    31081 caagaagtaa ctattataga tatagcttct tctcctggag gagtagatta ttctgttgtt
    31141 aaagaagcag ggattaatgc gactctttgc cttgggcttc ctggtaaata ttcaccaaag
    31201 acatctggtg aaattcttgt taacgctata gaaaatatta taaatgaaag aagggattag
    31261 aaatgagact tgaaggactt acaattggag taggatttac tggttcgttt tgtacttatg
    31321 ataaaatatt tatagagtta gagaatttgg ttaaagaagg agctaatgta cacactatat
    31381 tttcagatgt atcacaaaat atcgattgta gatttggaaa ttctgaagag tttatgaaaa
    31441 aagcatatga acttacagga aataaaccta tagttacgat tgaagaggca gagccatttg
    31501 gacctaaagg aattgctgat attataataa tagctccttg tactggaaat actgctgcaa
    31561 aattagcaaa tggtattaca gattcaccag tattaatggc agcaaaaggt catcttcgta
    31621 atgataaacc actagtaata tcaatatcaa cgaatgatgc tctaagcttt aatttcaaaa
    31681 atattggcat tctccttaat tctaaaaata tatattttgt accttttggt caagataatt
    31741 gcaaagcaaa gcctaattct atgattgcac atactgagct aataattcca actatagaac
    31801 ttgctctaga gaacaaacag ctacagcctg ttataaaaag tccacatcaa tagaattggt
    31861 tcttataata ttatgtatta gatacaatac tagcaaagag gctgtctcaa aatagagaga
    31921 agcctctttt tcttgttgaa atgatgaaaa tatatttaat ttcatacaaa aaaatgcctc
    31981 atggactaaa aagtctattt tgagctaatc tccgtaattt ttgctatatt aaaattattt
    32041 acttaatcca atttttaaat tagaatttat atcgttttga ctcttcaaat atcaatctaa
    32101 ctgttgttga tacaaaagaa gaggacataa ttaaaacaaa tactgatagt cctctaataa
    32161 acaaattatc tacataataa gttactattc caatacctcc aatcgttaaa attacactta
    32221 taatcaaaga aaaaacgcta attgttttta atttttccat gcttatcctc cttcttataa
    32281 acttcaaatt tatctatata atattaaata tattttattt catattatat aa
//
LOCUS       DBGJFU010000035        32313 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.035, whole genome shotgun sequence.
ACCESSION   DBGJFU010000035 DBGJFU010000000
VERSION     DBGJFU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.035"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..606
                     /locus_tag="ACOIAS_001562"
     CDS             <1..606
                     /locus_tag="ACOIAS_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2701277.1"
                     /translation="ISGYLTKKLQDLNVDTIRTDILTSPTVTDVIVWNIEQSGTDTFS
                     ATYEVDQQIKEGEQTTTVKATYTVKVHVDADRDMVIIQNPTLAPAIEKSDYEPKTPEA
                     DASVDADTVNDATAFLETFFKLYPTATAKELAYYVFGNVLEPIGRDYLYSELVNPVLT
                     KDGDNMKVKVAVKFLDNQTKATQVLQYELILHKDSNWKIIM"
     gene            819..1676
                     /locus_tag="ACOIAS_001563"
     CDS             819..1676
                     /locus_tag="ACOIAS_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2701278.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVTSLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            1754..2335
                     /locus_tag="ACOIAS_001564"
     CDS             1754..2335
                     /locus_tag="ACOIAS_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2701279.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            2439..3284
                     /locus_tag="ACOIAS_001565"
     CDS             2439..3284
                     /locus_tag="ACOIAS_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701280.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            3281..3964
                     /locus_tag="ACOIAS_001566"
     CDS             3281..3964
                     /locus_tag="ACOIAS_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701281.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIICFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVEHM
                     WKSLGGVKL"
     gene            3961..4659
                     /locus_tag="ACOIAS_001567"
     CDS             3961..4659
                     /locus_tag="ACOIAS_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701282.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            4671..5582
                     /locus_tag="ACOIAS_001568"
     CDS             4671..5582
                     /locus_tag="ACOIAS_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HGZ2701283.1"
                     /translation="MEKGRLLQNKNMNKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHCSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALNIFKESINKMVDHS
                     CDNATETRIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            5634..5774
                     /locus_tag="ACOIAS_001569"
     CDS             5634..5774
                     /locus_tag="ACOIAS_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701284.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            6065..6691
                     /locus_tag="ACOIAS_001570"
     CDS             6065..6691
                     /locus_tag="ACOIAS_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2701285.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            6720..6779
                     /locus_tag="ACOIAS_001571"
                     /pseudo
     CDS             6720..6779
                     /locus_tag="ACOIAS_001571"
                     /inference="COORDINATES: protein motif:HMM:NF025568.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6813..7529
                     /locus_tag="ACOIAS_001572"
     CDS             6813..7529
                     /locus_tag="ACOIAS_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HGZ2701286.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            7679..8275
                     /locus_tag="ACOIAS_001573"
     CDS             7679..8275
                     /locus_tag="ACOIAS_001573"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HGZ2701287.1"
                     /translation="MKILWIIIIMADNLLPLLLARFYPNYKHKEMALSVLGSRQSPVK
                     WIYNVWCIISGVVFCIAPYALYQENDSGWAIAIWMLLAIYGIGCEIISGVCPLNENRQ
                     DEDIITKIHGGTSAIGFMALLVVPLLFAILQFQTTKVVLGAISLVCFTIAFIFFCFFI
                     MGEKEKFANTVLRYGGLWQRLVLISCYIPLFVWSVSNQ"
     gene            8362..8553
                     /locus_tag="ACOIAS_001574"
     CDS             8362..8553
                     /locus_tag="ACOIAS_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HGZ2701288.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            8577..8807
                     /locus_tag="ACOIAS_001575"
     CDS             8577..8807
                     /locus_tag="ACOIAS_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701289.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            9074..9493
                     /locus_tag="ACOIAS_001576"
     CDS             9074..9493
                     /locus_tag="ACOIAS_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HGZ2701290.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            9496..9750
                     /locus_tag="ACOIAS_001577"
     CDS             9496..9750
                     /locus_tag="ACOIAS_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2701291.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            10125..10328
                     /locus_tag="ACOIAS_001578"
     CDS             10125..10328
                     /locus_tag="ACOIAS_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2701292.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            10406..11596
                     /locus_tag="ACOIAS_001579"
     CDS             10406..11596
                     /locus_tag="ACOIAS_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2701293.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPTGKRDDKSLREKEKQIKKDLDDGIDTIGGKMTVCQLYAKKNNQKKNIKRN
                     TEIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFHFKLSDVLEDDTEPKVILTPEQEEKLLSFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHE
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(11853..12653)
                     /locus_tag="ACOIAS_001580"
     CDS             complement(11853..12653)
                     /locus_tag="ACOIAS_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701294.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(12848..14446)
                     /locus_tag="ACOIAS_001581"
     CDS             complement(12848..14446)
                     /locus_tag="ACOIAS_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2701295.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            14774..14950
                     /locus_tag="ACOIAS_001582"
     CDS             14774..14950
                     /locus_tag="ACOIAS_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701296.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            15124..16254
                     /locus_tag="ACOIAS_001583"
     CDS             15124..16254
                     /locus_tag="ACOIAS_001583"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HGZ2701297.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            16495..16845
                     /gene="eutS"
                     /locus_tag="ACOIAS_001584"
     CDS             16495..16845
                     /gene="eutS"
                     /locus_tag="ACOIAS_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGZ2701298.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            16853..17302
                     /locus_tag="ACOIAS_001585"
     CDS             16853..17302
                     /locus_tag="ACOIAS_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HGZ2701299.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            17404..17979
                     /locus_tag="ACOIAS_001586"
     CDS             17404..17979
                     /locus_tag="ACOIAS_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2701300.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            17972..19381
                     /locus_tag="ACOIAS_001587"
     CDS             17972..19381
                     /locus_tag="ACOIAS_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HGZ2701301.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            19565..20998
                     /gene="eutA"
                     /locus_tag="ACOIAS_001588"
     CDS             19565..20998
                     /gene="eutA"
                     /locus_tag="ACOIAS_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGZ2701302.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            21016..22380
                     /locus_tag="ACOIAS_001589"
     CDS             21016..22380
                     /locus_tag="ACOIAS_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HGZ2701303.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            22393..23274
                     /gene="eutC"
                     /locus_tag="ACOIAS_001590"
     CDS             22393..23274
                     /gene="eutC"
                     /locus_tag="ACOIAS_001590"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HGZ2701304.1"
                     /translation="MNEKDLKALVEQLVGQMLGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            23294..23947
                     /gene="eutL"
                     /locus_tag="ACOIAS_001591"
     CDS             23294..23947
                     /gene="eutL"
                     /locus_tag="ACOIAS_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGZ2701305.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            23958..24659
                     /locus_tag="ACOIAS_001592"
     CDS             23958..24659
                     /locus_tag="ACOIAS_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HGZ2701306.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            24662..26131
                     /locus_tag="ACOIAS_001593"
     CDS             24662..26131
                     /locus_tag="ACOIAS_001593"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HGZ2701307.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            26227..26514
                     /locus_tag="ACOIAS_001594"
     CDS             26227..26514
                     /locus_tag="ACOIAS_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HGZ2701308.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGATAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            26641..27402
                     /locus_tag="ACOIAS_001595"
     CDS             26641..27402
                     /locus_tag="ACOIAS_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HGZ2701309.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            27415..28044
                     /gene="eutD"
                     /locus_tag="ACOIAS_001596"
     CDS             27415..28044
                     /gene="eutD"
                     /locus_tag="ACOIAS_001596"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization phosphate
                     acetyltransferase EutD"
                     /protein_id="HGZ2701310.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            28086..28811
                     /locus_tag="ACOIAS_001597"
     CDS             28086..28811
                     /locus_tag="ACOIAS_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HGZ2701311.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDGFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMNGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDILNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            28824..29096
                     /locus_tag="ACOIAS_001598"
     CDS             28824..29096
                     /locus_tag="ACOIAS_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HGZ2701312.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            29089..29640
                     /locus_tag="ACOIAS_001599"
     CDS             29089..29640
                     /locus_tag="ACOIAS_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HGZ2701313.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            29652..30743
                     /gene="eutH"
                     /locus_tag="ACOIAS_001600"
     CDS             29652..30743
                     /gene="eutH"
                     /locus_tag="ACOIAS_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGZ2701314.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            30745..31218
                     /locus_tag="ACOIAS_001601"
     CDS             30745..31218
                     /locus_tag="ACOIAS_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGZ2701315.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            31731..32252
                     /locus_tag="ACOIAS_001602"
     CDS             31731..32252
                     /locus_tag="ACOIAS_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2701316.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
BASE COUNT        12232 a         4339 c         5956 g         9786 t
ORIGIN      
        1 atcagcggtt atctaacaaa gaaattgcag gacttgaatg ttgatactat tagaaccgat
       61 atactgacta gccccacagt tacagatgta attgtgtgga atatcgaaca gtcgggaacg
      121 gacacttttt ctgctaccta cgaagtagat cagcagataa aagagggaga acagacaaca
      181 acagtcaagg caacctatac ggtaaaagtc catgtagacg ctgacaggga tatggtaatt
      241 attcagaacc ctacccttgc accagcaatc gaaaaatcgg actatgagcc aaagacacca
      301 gaagcagacg caagtgtaga cgctgatacc gtgaatgacg ctaccgcatt tctggaaaca
      361 ttctttaagc tgtacccgac agccacagca aaagagcttg cctattatgt atttggaaat
      421 gtgcttgaac ctatcggcag ggactacctt tattcagaat tggtaaaccc agtacttaca
      481 aaggacgggg ataatatgaa agtcaaggta gctgtaaaat tccttgataa tcagacaaag
      541 gcgacgcagg tattgcagta tgagcttatt ctacataagg atagtaactg gaaaattatt
      601 atgtaagaca ttttgttggc aggatctatt ttcggatata gtctgccttt tctgtagcaa
      661 atgtactgca ctgacaagct atttcattgt attttaagca attaaacctt tcttgacaat
      721 tttatcttaa atcatatatt tatggatatt gactttctgc ttgcaataag ctataatatg
      781 actataaaat caaatcagtc atataggagg tatgtggtat gtataaagaa gttggtattt
      841 cttcaaataa tgttccgata acactttcta tctggtatac agaaagaagt agtcctacca
      901 ttgttttttt gccgggtact atgtcacacc ctctcatgta tgagaatttt ctatgtggtt
      961 tgagtgaaag aggatttaat ataataggca ttcactatct ttcacacgga aaaagcccta
     1021 aaatcaaaaa aaattataca atggaagatt tgctgcataa tgtatatgac gcaacaactt
     1081 acggaataga aaatttcggt gaaaacatag ctgtcatggg ttccagtcaa ggcgggattt
     1141 tagctgcaat ggcggcaggt agggacacaa ggttaaaagc cgtttttcca cataatatca
     1201 tgctgaccac attaaaagaa actatgagct taacaaaata tcccacatgg tcgcaccgtt
     1261 ttatggggct gatacagtgg ggctttcgtg taggtggaaa attgctgcct aactacaagg
     1321 ttccaggaga cgcttattta gactatgaca gagtatttta tagtgaacag gcaaaacaag
     1381 attgcttaaa tgaccctatg cttttaccct attatccttt aagatttgtc accagcttat
     1441 ttaatgctga cttatcgtgc ctggagaatg gggctataca atgtcctgtt attttaatta
     1501 ctgccaaagg cgacccgctt tttagctttg attataccaa caaagtattt gatttaattc
     1561 atgtaccgca aaaggaatta cttgctttag agcttaatcg tcacatgatt tttaatgagg
     1621 acacggacac tacaataaac gcattggaag ctaccttgca taaatatctt ggatagcata
     1681 ttgattatat aatcagtaaa tgttatactt ttcatgacga tataaatatt ttagtcataa
     1741 ggaggttatg agtttgccac gatttacgga acaagaaaaa gaaatcataa acagtaaact
     1801 tttgatagag ggagaaaagc tgtttgcatt acacggctta aaaaaagtaa cagttgatga
     1861 tttagtagct gctgtaaaca tatcaaaagg ttctttctat gctttttatc ccagtaaaga
     1921 acatctatat gttgaaatca attttcgctt acaaaaagag ttatttacaa gtatagaaac
     1981 aaccattaaa aagaaaaagt ataagaacca tagagattta gcaaaggacg ttatcatgct
     2041 aagtttaacg ggggtgatta cttcgcctat tctttcacag attgatttat ctttaatgga
     2101 ctatctgcaa agaaaaatat cgtcagatat ttttgaaaat catatgtaca gtgatattcg
     2161 tatattggag atattggaag atttaggggt tgtatttttg gttccgcata cggttattat
     2221 aaaatccctg tattctgtct tatcctgttt ggaacaattc aaagaggacg aagaactgaa
     2281 tatgatacaa aatcttttag tgaatggtat tattcagcaa gtagtagcag agtaacaatt
     2341 ttgtttggtt atttgacagg caggtagcca aacaaatttt ttacagttta tatgactata
     2401 tatctataaa cgtcataaag ttaaaggagg aatgaataat gattgatgta aaaaaactgt
     2461 attttagcta tactgacaaa ccgtttgtgg agaatgtaag tttccatgtt gggagaggag
     2521 aaatttttgg ttttttagga ccctctggtg ctggaaaatc aactattcaa aaagttttaa
     2581 ctgggcttaa tacaagatat aaaggcagtg taaaagttgc aggtacagaa ataagggagc
     2641 atacaaatca gttttatgaa aatattgggg tagactttga attttcaacc tgctacgaga
     2701 aattcacagc acgacaaaat ctcgcctatt ttgcttctct atatgaaaag caacctcggt
     2761 ctattgatga attattgcat atggtaggct tggaaaatga cggggacaaa aaagttgctg
     2821 acttttccaa aggtatgcgt tctcgcttga atttcattaa agcgttagtc catgaccctg
     2881 atatattatt ccttgatgaa cctacaagcg gtcttgaccc tacaaatagt cgtttaatga
     2941 aagatattat tcttgcggaa aagaaacgcg gaaaaacgat tatcattact actcacaata
     3001 tgtttgacgc aacagagcta tgtgaccaag tagcctttat cgttgccgga aaagtcagtg
     3061 cattggatag cccgcataat ttgattatgt caaggggagc tgccaaaata caatatacct
     3121 actatgataa aggcgagaaa accggagaat gtctgcttga tagaaccaca gaagataagt
     3181 tgttaaaaag tttaatttcc gaaaaccgtt tgctttctat tcatagtagt gagcctacat
     3241 taaatgatat ttttgttgat attacaggga ggacacttca atgagattgg gacgtttaat
     3301 ttgtggtgat atacatttcc aatggaaata tggtttctat tttatctact ttatattaac
     3361 agttttatat gtgtgcggga ttgccgcttt gccggaacat tggaaaacgg atattgcttc
     3421 tattatgatc tactctgacc cggcagcaat gggattattt tttatgggag cgattgtcct
     3481 cttagaaaaa agtcaaaagg tgttaaatgc tatggtggta tcccctgtaa aaatatcgga
     3541 atatatactt tcaaaaacag ttgcgttgat tgctatttct acggtaatcg cattgatatt
     3601 aggggtagtt tccggcagca atcatttact gggtatcgca gttggtacgg ctttgacctc
     3661 agcaatcttt actatgcttg gaattattgc agctacaaaa atttcaaact tgaaccaatt
     3721 tctcattgtg attatgccga tagaaattat ttgctttgta ccacctattg ttggtctgtt
     3781 tgtgaagtta ccatatctgt tccgcttttt tccattcaca gcttgcatga accttataac
     3841 tggaaaaagt gttttactat cgtttgatat ggtacttgta attgctacgt tgattatcct
     3901 ctacatagtt gctcgacata cagttgagca tatgtggaaa agtttgggag gtgtaaagct
     3961 atgaaaaaga tattatccag caccatacag atatttaagc aaatcaaaag tgaccctatg
     4021 atgtttgcgg cttgcttctc cccttttatt atggggactt taattaagtt tggtattcca
     4081 tttcttgaaa gaataacgaa gttttcttta caagcgtatt atccgatttt tgatttatta
     4141 ctttctatca tggctcctgt attgctctgc tttgcatttg caatgattac attggaggaa
     4201 atcgacgata aagtgtcacg gtacttttcg attacccctc ttggtaagtc ggggtatctc
     4261 tttacaaggt tgggagtacc ctcaattatt tcggcggtca tcgcttttat tgtactgtta
     4321 cttttttcat tagaaaagtt gtccattgga atgacaatat gtttggcgct tctcggctca
     4381 gtacaggcaa ttattgtttc acttatgatt atcacattat caagcaataa attagagggt
     4441 atggcagtta caaaactagc tgcacttaca ttgctcggaa taccagcccc cttttttatt
     4501 gatagttact accagttcgc ggttggcttc cttccatcat tttgggtagc gaaagccatg
     4561 cagaatgaag cggttcttta tttctccata ggattggtgg tagctttagt ctggtactac
     4621 ttccttacaa aacgtctgtt tcggaagctg gcaggataag gaggcatatt atggaaaaag
     4681 gtagacttct gcaaaacaaa aatatgaata aggctattca ggtttccaaa attacactaa
     4741 tcatcaatct gagtttatcg ctacttaaat ttgctgccgg atatatagga aagtcaagtg
     4801 ccatgctgtc ggacgctgta cattcggctt ctgatgtagt aagcacaatc gttgtgatgg
     4861 ttggaattaa aatatcagaa aaacagcctg atagagaaca tccttatggg catgaacgta
     4921 tggaatgtgt ggcttccatt attctctctg ttgcattggc tataacgggt gctggaatag
     4981 gatattcagg tatcaagaaa atcttttcag gacagtacaa tacgctttct gtccctagtg
     5041 gaatcgcact tacggcagca gtcttatcta ttgttataaa agagtggatg tactggttta
     5101 caagaagtgc tgcaaagcat acaaattctg acgcacttat ggctgacgca tggcaccact
     5161 gctctgatgc actctcgtcg gttggttcct taattggcat tttaggagcg cgtttaggct
     5221 atgctattct cgatcctatt gcaagcgttg tgatttgcgg ttgtatctta aaagctgctt
     5281 tgaacatttt caaagaaagc attaacaaaa tggtagacca tagttgtgac aatgctactg
     5341 aaacaaggat tagggaagtt gtgcttcagc agcaaggggt tgatggtatt gatgaattaa
     5401 aaacccgcat gtttggagcg aaaatgtatg tagatattga aatattagct gatggtaatt
     5461 tagctcttta tgacgctcat agaattgctg agggagtcca tcaaacgatt gaaaacaact
     5521 ttccacaatg taagcattgt atggtacatg ttaatacgaa tgaacttctc tattctgcat
     5581 aggacaggga agatgattta aggctattga aagggaaagg agggaagtag gttatggaac
     5641 aatactggtg gattatcatt gttgtggttt tgattgtcgt tggcttactt aaaattgtaa
     5701 ttatgaagaa gtataaccaa aggagaaatt ctaacaatcg ttcaaaagat agcgaggacg
     5761 acaagatgta ttaaggttta ttgaaatctg ttatgttgta gaatgatgtt tttagatact
     5821 agaaagtgct ttaagggggg ctaacgttaa ggaaagcttt gagtacaatt ctttacaatc
     5881 aacagtcttt tgcatagaga aatgtatgta ggaaatatgg tgcaaggcac aaaacaccag
     5941 caattgagaa atcaacctat gaaccaaagg cacactacat tttatactac ttgacaagta
     6001 aacgaacatt tactttaatg gtttaaagaa gtaaacgttc gtttactaaa gaaggaggtt
     6061 gtaaatgtct gacacaaaag aaaatatatt aaaaattgca ctacacttat ttgcagtgaa
     6121 tggttatgag gctgtttctg ttagtatgat tgcggaagcc ttgggaatga caaaaggagc
     6181 attatataaa cattataaaa ataagcaaga tatttttgac tgtatcgtag aacgtatgta
     6241 tcaaattgat aataagcgag ctaaggatta tcaagtacca gaaagcacaa tagaggtaac
     6301 cccaaatgca tacagaaata caactattgg aagtctaata acatttactc ttgcacaata
     6361 cgatttttgg gtgactgatg aatttgcgtc taattttcgc aaaatgttaa tactagagca
     6421 atatagaaat ttaaaaatga atatgcttta tcaaaaatgt cttgtaaatg gtcctatcag
     6481 ttatatagaa gatatattta atgaaatgat tgaaaaaggt gttcttcaag agtgtgatac
     6541 aaaacagctt gctattgaat attatgcgcc attatttcta ttgataagtg tagcagacgc
     6601 agttgaaaat tgttgggatt taaaaactca attaactaca cacgttaaac aatttgtcaa
     6661 gaaatatatt ataaatgaga aagtaaaata atcctttatt ctgtcctaaa tataaaccta
     6721 actttttaat ttcagcagag aaactcaaaa tgaatattgt tataagtcac aaatattaac
     6781 tattaaaata ttacaataac ggagatgaga aaatggaata tattcgcagt aaaaaatatt
     6841 ttacacccga cttacagaca aaaattatgg ggccgaatcc tataaaatta caagaggaac
     6901 tattacaaaa tcataaaatc ccgtctggta gctatgtctg tgaccttgga agtggacaag
     6961 gtttaacttc tgtatttctt gcaaaagaat atggatttgt tgtttttgca gttgacttat
     7021 ggagtgagcc aaaagaaaat gaagaatttt tcaaaaaaat gggattatct tctgcacaaa
     7081 ttaaatctgt aaaagctgac gccaatgaac ttaaatttga gaaagagttt tttgatgctg
     7141 tagtaagtac agattcatat aattattttg gacgtgaccc tatatattta gatgaaaaat
     7201 tgcttccatt tgttaagaaa ggtgggtttg tttatatcac agttcctggc atgaagaaag
     7261 attgccatga gaaacttcca gaagagttat tgttatcatg gacaccagaa gaacttgatt
     7321 atatacatga tataacatat tggtctaaca taattagtca ttgtaaaaag gcagatttaa
     7381 tttcaatata tgaaatggaa agtaattcgg aagtttggtc ggattggata aagcaaaata
     7441 atgaatatgc aataaatgac cgtaaggcaa tagaagctgg cggtggagat tatctgaatt
     7501 ttattgctat aattctacaa aaaaaataaa ttctttagaa ccaaacacta taatttgcgt
     7561 tctaatatgt ttaccgattg acatcaataa ttttatcttt gagggtaatg atttgaaaat
     7621 agaagaatac aagaagatac gaaattgata aattagaatt tgatgggatg gtgatattat
     7681 gaaaatatta tggatcatta ttattatggc agataatctg ctgccgttac tgcttgctcg
     7741 tttctatcca aactataagc ataaagaaat ggcactatct gtccttggct ctcgtcaaag
     7801 cccggtgaaa tggatatata atgtgtggtg cattatatca ggtgtggtat tttgtatcgc
     7861 tccatatgcg ttgtatcaag agaacgatag cggatgggca atcgctatat ggatgcttct
     7921 tgctatatac ggcatcgggt gtgaaatcat ttccggagtg tgcccgttaa atgaaaatag
     7981 acaagacgaa gatataatta caaaaattca cggcggcaca tcggcaatcg gatttatggc
     8041 cttgcttgtc gttcctttgc tctttgcaat tctgcaattc caaacaacaa aagtagtgtt
     8101 aggcgcaatt tcactggtgt gctttacaat agcattcata ttcttctgct tttttattat
     8161 gggtgagaag gaaaagtttg caaatactgt tttgagatat ggcggtcttt ggcaaaggct
     8221 tgttttaata agttgttata taccgttatt tgtttggtct gtttctaatc aataaaattt
     8281 agtttatagg aataacaaat tccagtttgt aaaagtcaat aaatccaaag ataaatttgt
     8341 atttgaaagg agttttgaag aatgaaaaaa tatgagtatg tagaaattaa ctataatgcg
     8401 aaaggagttg tattcctttg tactgataaa cacaaagaaa ttattgacag ttacgcaaaa
     8461 acggggtatc actacgcagg gtttgtaccc acagaaattg acgcaaaagg ctgtatgcga
     8521 aagatagatt taatttttga aaaagaagat taacttattt tagggagttg gttctaatga
     8581 taggacttaa aagacgagat atgaattata ttatgaaagt tggtgctaaa gctcaaggtg
     8641 tttctgtatc tgaatttagg gctatgctac aagataccat agacaaaact atggaaagta
     8701 cagacccaga cgtgcaggca aattttaaac gatactttgg aaataagcgc ccaacaccag
     8761 aggaatatat ttatacgatt accaaaaaga caaaggttaa ggtttaaatg ttgtaaaggg
     8821 ggcttgccgt atgaaacgct ttatgtacaa ttcagtatat tcaagcccaa tttaacagca
     8881 tactatttac aattaaatac gctataccag tagctcgtac aaaatttata ttttgtgcgg
     8941 gcttttttaa tacccgaaaa gtttttttaa ttattttcat ttttttggaa gcaaatcaca
     9001 ccttgctgta cagatatggt gcggaaagag ggataaacac ttttcaattc atagctggaa
     9061 aggaggtgag attatgaaac cgtctgactt ccaaaaaaca attcagtgtc agtttgaatg
     9121 taagctgaaa aaagttgtca aaggtattat ctggaactac cgtaaagaat taaaacgaag
     9181 aaaaaacaag gaaatatctt ttagcgaatt accagacatt acggtagaaa gtctggcaac
     9241 atgggacgac tacgaaacaa actacacaat ttttaatgtg tgtggcatag atattaaagt
     9301 ccttgatgat gaacttgcag aagctctcaa acagctaccc gaaaaaaaac gtaacaatgt
     9361 attgatgtat tacttcttgg aaatgagtga tacagagatt gcagatatta tgaattatac
     9421 cagaggggct gtgttcaaac acagacaccg tgcactagaa actatgaaag agatactaaa
     9481 ggagaagaaa tagaaatgaa gaaaaagtac aagcacccgt cttattattt gatttcatca
     9541 gctatggatg gggatgaaac tgcgattgag aaattactgg cattctatga cccgtacata
     9601 tcaaagtgtt gtttacgtcc tttatatgat gaatacggca atgtttatat tgttgttgat
     9661 atggaattaa agggacgtat tcgagaagcc ttaatcaaaa tgattttaga ctttgagatt
     9721 gatatagata tagaaaccga agaagaataa gcaaatacca gcagggcgtg attggttaaa
     9781 ttcccttctt tcctgccctg ctcctgcttt tatacatgga agcctacggc tttcgccgat
     9841 tgttctttga caactgaata aagcagacag atacgtttgt gatacagcga gccacgggga
     9901 ctgtacgcca tgacctttcc gacaagaaag cgagcgaccc acgcagttga tccgagagcg
     9961 acttgtggaa tggtcgtttt gccatgacct gctatcacag aataatgata cgtccgcatg
    10021 gtgcggttct gcccacaaga atgggaatag ttgagatact aacggagctt tccaaagccg
    10081 tctgtctgct tatgataaga cacacagaaa ggagttgttg caacatgaac aatactgatg
    10141 tgccgatttg ggaaaaatac acccttagta tagaagaagc gtcccgatat tttcgtatcg
    10201 gggaaaagaa gttgcgtaaa cttgccgagg aaaatctgga ctctggttgg gttatcgtta
    10261 atggaaaccg tattcagata aagcgaaaac agtttgaaaa aattattgat acactggacg
    10321 aaatctgatg tcttagagcc gtagatatgg tataatatta gtagcgtatc acggctcatt
    10381 ccatgacgga aaggagcttt acactatgtc aaaaatgaaa agacaagaca gtaaaggacg
    10441 caatttgcgt cttggagaga gccagagaaa agacggaaga tacgtttata aatataccga
    10501 tatattcggg aagccacagt ttgtatatgc ttggaaactt gtaccgacag acaaaacacc
    10561 tactggaaaa cgtgatgata aatcattgag ggaaaaagaa aagcagataa aaaaagacct
    10621 tgacgacggt atcgacacta tcggcggtaa aatgacagtc tgccagcttt atgcaaagaa
    10681 gaacaaccag aaaaagaaca tcaagagaaa cacggaaata ggacgacagt atcttatgaa
    10741 cgcactagaa aatgacccat taggtatgag ggcgattgat acagtcaaac agtccgacgc
    10801 taaagaatgg gctatcagaa tgaatgacaa aggttattcc tataagacaa tagataacta
    10861 taagcgttcc ttaaaggctt cattctatat ggcgatacaa gacgactgta tcagaaaaaa
    10921 tccttttcat ttcaagctaa gtgatgttct ggaagatgat acagagccta aagttatctt
    10981 gacaccagag caggaagaaa aacttctttc ctttatggaa acggataatg tttacagcaa
    11041 gtatcgtgat gaagttattc ttctgctgga aacgggactt cgtatctctg aattatgcgg
    11101 actgacaaca cacattgata tgcttaatcg tgtgattaac atagaccacc agctattacg
    11161 tgatacagag gtgggctact atatttccac acctaagaca aaaaacggca agcgagagct
    11221 tccattgaca gaaagagcat atcaggcact tgaacgaatt ttaaagaacc gaggaaaagc
    11281 tcaaccgctt gttgtagacg gttatagcaa tttcttgttc ttgaaccgtg agggcttgcc
    11341 aaaggtagca ggaaattatg aaagtatggt gcgtgggctg attaagaagt acaacaagac
    11401 ccatgaggac aagttgccga acatcacgcc ccactcattc agacatacct actgtacgaa
    11461 catggcaaat aagggaatga accctaacac cctgcaatac atcatgggac acgcaaacat
    11521 aaccatgaca ttagggtatt atgctcacgg tactttccag tctgcaaagg cagagttgga
    11581 aaggctggct tcttaatatc gaaaatgtat cttactactt atttactact tttggttgca
    11641 ttttcatgct ggtaaatgcc agcttatgcg aggtatcttc caaaaagaaa atgcccatac
    11701 agcccgaaaa tacgggatat atcggcattt accaacttat gaaaagataa tcagaaatta
    11761 gtaagttttt atatcaaaaa aatgattact gtaaagaaat cattttttat tataatcact
    11821 gatacatcaa atattctaaa taaaattttt atttatctgg aattgtatat tttttctata
    11881 atattaacta cagcgtacat tgcccaagca cagatagcca atacaaatac tcccatcatt
    11941 actaaatcta atttaaatac ttgagaacca tatactatca agtagcctat tccatatctt
    12001 gaaactaaaa attctccaac tattacacct acccatgcca taccaatatt aattttagtt
    12061 aaatttatta aatttcctgt gtttgcaggt aatatgagtt taaataatat ttgagattta
    12121 gtagctccaa aactttttaa catcttaatt ttttcttcat ctatattcat aaaataatta
    12181 taagctgaaa gtatagtaac aacaacagaa atcgttacag ctgttacgac gattccttct
    12241 attccagcac ctacccaaac tataattata ggagctaaag cagtcttagg aagagcattt
    12301 aaaactacca aaaatgggtc taatattttt gataattttt ctgaccacca aagagcaatt
    12361 gctaccaaaa tacctaatac agtacctata acaagaccaa gaacagtttc atatacagat
    12421 attccaatat gtttaaataa ttgaccacta aatgcatatt gaataaataa attgtaaata
    12481 tcacttggtt tgctaaatag aaatgtattt ataacattta agtcagctaa aagttcccag
    12541 attacaataa aaccaattaa aattaatagc tggtagaata atacatttct tttttctctt
    12601 tttacacctt taacatagtt tatatatcct tcagatcttt tattaagcct catgtgtatc
    12661 tagctccttc cataatatgt cgaaatactt ttgaaaatca ggaactttac gtgttttgag
    12721 aggcgttttt gttccatcca tgttaagttc aatatcgtaa atatttttta tggtagctgg
    12781 tcttttagat agtattgcaa ccttatctga tatgcttatt gcttctgcta tagctaaaaa
    12841 agtgcaacta attgcttaaa gaaacagaat tatcaacttc agcacccata taaacaatat
    12901 taagatttcc taaactgcta tcccaaacga tactattaat aatagtagat aataacaatc
    12961 tcttatcatc taaactggca ttatcaactt catcattaaa tctcttcaaa ttactaacaa
    13021 taatatcaat attaatactg tcactactaa tattgatgtt actagattcc aaaatattaa
    13081 tcttatcctt taaaatacta atctcattat ttaggctctc aatctgggaa actatataat
    13141 cagaagcagt actaccagta ttttttgcaa gctctataac taaattttta ataagtcttt
    13201 ccttatctaa aatttgattt ttaagtaaat tagtctcaga tttaaaaata ttaaaaacat
    13261 tatcagtctt aatgttatta ctattgaatg cacttataat agactcttct ttacaattct
    13321 taattttatc tataactaaa ctttcaacta atggaccatt aagattttta cagttgcaac
    13381 tatcagaccc aagtgttttt ttagtaccac aaatataata atactttaaa tccttgtcag
    13441 tttttttgcc tttataggta attctcatat tagaaccaca tttaccacat tttataagac
    13501 cagataaaag agcagttttt ccagttgata tccttggagt tttatcacga tttttatcta
    13561 attgattttg tacttcaagc catatttcag gttctatgac tccatggtgt tttgcaattg
    13621 ctgctattga gttagatgta tttttagcat aggtaaggat tccacatttt ctatctggag
    13681 tacctacaac atccatacca ctatttttaa ggtaatctaa aatttcatca ttagctttta
    13741 catatgctgg atttctaagt attctagata aagaactagg gtcaaatttg ccaccacgag
    13801 gaccccttat tccattatta tagacatatt tatataactt agataaggac ctaagttcta
    13861 aatatttatt gtatattagc tttacaatct ccatttgttc atcatttact tgaagcttat
    13921 acatacttct ttcttttaga ttttcatcta agaatgtgat tttttcactg ttgaatccat
    13981 gaggtgtatt gcctccaagc catctaccac tacgggctaa ttcatacata ttgtctttta
    14041 tacgttctgc tattgtttct ctttctaatt gagcaaatac acttgatata aacatcatag
    14101 ctcttcccat tggagttgaa gtatcaaatt gttctttgat agatataaat gatatgctta
    14161 gttcatttaa tttttctatt aaagtagaga agtcagatac atttctcgat atcctatcta
    14221 atctataaca aactaggtaa ctaaatttct tatctttggc atcctgcatc attttcttaa
    14281 attgtggtct atctatattg ctaccactaa atccctcatc ttcatatatc aaaaaatctt
    14341 ttacaccaat agactttgca taatcaatac ataattgtat ttgattttct atagaatcac
    14401 cttttccagt aaatcttgat tttcttgaat aaattgcagc tttcaaaatt aaaaccccct
    14461 ataagttagg atatatattt gaaactattt atctacaacc ttttaagtaa acttattaga
    14521 atgaatttat taattaggta aatttatata attctacttt ttataatata tgactattta
    14581 ttttatttct ttactattca agttctaaat caataataaa ttttgatatt taattgtatc
    14641 aggacaaatc tctaatttca tagattatta atataaaatc ataggttagt gtgaaaaagt
    14701 tgaaatttaa atattttaaa agataatttt tttgaactaa taggtataac aaaaatttat
    14761 agggggattg catatggaaa tcaatgttat agaaattttc cctaaagata aagctaaact
    14821 taataaaata gaaatggata aagctagttg gtttgtaaat ataataagta aaaaatatcc
    14881 taaagaagct ttaaatgaag catttagtac tttagaaaaa gaattaaata taagtaaagc
    14941 taatacataa gctttgatta ttgattttat atactattgg ttttatacgc tatgacttga
    15001 cattatatta atcaaatagt atatttttaa atatagcaca atgaagtgtt aaaagagcaa
    15061 tggagctcca tagataaagt ctatggagct tttttgcgta tttaacttaa ggaggatagg
    15121 actttgaata actttgagat taaaactaga ataaaatttg gagaaggctc actaaaagct
    15181 ttgcaagaat taaaaaacaa aaatgtacta ataataacag acccttttat ggtgaaatca
    15241 aaaacaatag ataaaataac atctaattta ataaactcaa catatcaagt ttttgaagaa
    15301 atagtaccag acccaccaat agaagtagta gtagcaggaa tagaagcatt taaatctata
    15361 aatccagacg taataatagc attaggtggt ggttctgcaa tagatgcagc aaaatctata
    15421 atggactttt ctaaaaaaat attaaaaatt aacgaagtag agtttatagc aataccaaca
    15481 acaagtggaa caggttcaga agtaacatca tttgctgtaa taacagatag tcaaaaaaaa
    15541 tgtaagtatc cattagtgag tgatgattta cttccagata tggcaatact agacccagaa
    15601 cttgtaaaaa ctgtaccaaa ttttataaca gcagatacag ggatggatgt attgacacat
    15661 gctatagaag cttacgtatc tacaaattct aatgactttt cagatgcact agctgaaaaa
    15721 gctataaagt tagtatttaa atacttaata aaagcatata aagatggaaa tgacatagaa
    15781 gctagagaaa aaatgcacaa tgcatcttgt ttagcaggta tggcatttaa ccaagcatca
    15841 cttggcataa atcattctat tgcacatgta ttaggtggaa aattccatat accacatgga
    15901 agaacaaact ctatattact tcctcatgta atagaatata attcatcaat agtaggatat
    15961 actaacacaa cttactctgc tacagctaaa aaatatgcac aaatagcaga attagtagga
    16021 cttgggagtg ctaatataag aatatctgtt agaaatttag taaatgaaat aaagaaacta
    16081 caaaaagaaa tgaatatgcc aactaaattg acacaatgca aattaagttt agaagatata
    16141 actaatgaag aaagagaaat agcaaaactt gctataaaag atacttgtac attaactaat
    16201 cctaaggtac caactgaaga tgatataata aacatattaa agaacattaa ataactacat
    16261 tttattacat gtaaatataa tattatacaa atagggaaaa tgtttttaaa aatataaatt
    16321 atatattgac aaatataaaa cataaaacta taatgaaagt atcacaaagg cgtgatactt
    16381 tagtagaaag caatggagct tcttaggaat attaatatta ttcttatgga gctcctttta
    16441 ttttaatcaa atagcttttt aataaactat attcaaagaa agcagggaat aaatttgact
    16501 gaagaatcta agcaaagagt tattcaagaa tatgttcctg gaaaacaggt tacattagca
    16561 catatcatag ctaaccctaa tgaagatata tataagaagt taggattagt acttgataaa
    16621 aaagatgcaa taggaatact tacaataaca ccaagtgaag cgtcaataat agcagcagat
    16681 gttgctacaa aggcttcaaa tgtatcttta ggttttatag atagatttag tggttcagta
    16741 gtaatatcag gtgatgtaag ttctgtagaa tcagcgttaa atgatgtttt agaagtctta
    16801 ggaaatatgt taaacttctc atctacaaaa atcacaagga cataataata caatgaagaa
    16861 tataatattt atgggaaaga caggtagtgg taaaactacc ttatgtcaaa aactagacca
    16921 gttagaatta aagtataaaa aaactcaatc agtggaactt tataataatg caatagacac
    16981 tcctggtgag tatatggaaa atagaaacct atataatgcc ttaataacaa catcagcaga
    17041 tgctaaaatt atagcaatag tgtatgatgc cacacaagaa gaaaattata tagcaccagg
    17101 ttttgccagt atgttttgca aagacgtaat aggaattata actaaaataa ataaagctac
    17161 tgaaaacgaa gtcaatatag cttatgaaag actatcacta gcaggagcaa gtcaaatctt
    17221 taaaatagac acagttgata atattggact tgaaaaatta tttaaatatc tagaaaaata
    17281 taaaaatata gatgaagttt aaggggaaat atttttttat tcaaaaatat tattaaagta
    17341 tgataaaata aaaaaatgta taaactattt taagaagatt taatctttaa gcgaggtagg
    17401 aacatgggga aaaaaatact attggttgat gatgaaccac tgttaaggat ggatacaaaa
    17461 gatatattag aaagtcatgg ctatgaaata ataggcgaag caagtgatgg atttgaagct
    17521 gttgaaatgt gtaaaaaaca caaacctgat ttagttataa tggatataga gatgccaata
    17581 cttgatggta taaaggcagg caaaataata tgtaaagaaa atatagctgg tggagtactt
    17641 ttattaactt catttgaaga taaagaatac gtagaaaagg ctaaaagtat aggtgcttat
    17701 ggatatttag taaaaacagc aagtgaaaaa tcacttatcc caaccataga aatgtgttta
    17761 aataaggtaa atgaatttga aaaaatgaaa aaagatttag aaaaagtaaa ttctaaattg
    17821 aatgaaagaa aattagtaga aagagcaaaa ggtatactta taagagagtt taagatttct
    17881 gaggatgaag catacactag aataagaaaa ttaagtatgg ataaaagaac tacaatgatg
    17941 gaaattgcca aaatgattat aataggatac gatgactaat acgataaaaa aaatatcaac
    18001 tgcacaaaca tacttaaata ataacgaaat tgaaaaaatt atagaagtat caaaatcctt
    18061 agatttaatg gcttcttttt atgaagctga tgtttttata gatgtactaa gtaaatttga
    18121 tgatgaagca attgttgtgg cacatggtag acctaaagaa aagtcaatgt actgtgaaaa
    18181 tgttataggt aaaaaggctt taattgaaaa tgaaccagga gttataaagt gcttaaaaac
    18241 aggagaaaga gtaagagata ttaaagcttt aacacaggaa tataaactgg tcaaacaaaa
    18301 tattcagcca ataacactag atgacaaagt tttagcagta ctaatagttg aaaaagatat
    18361 aagtagtgaa gttaagaatg agtttaatgc tagtgaagac gaagaaaata aaaatttaca
    18421 tcagtttata aaacttattg gaaataatga ctttttagtt gataatttaa attcagccat
    18481 attaatattt gataaaaatg gaaaattaaa aattaaaaat cgtattgcaa ctgagatata
    18541 caaaaatctt gggtttggat atgatataca agatatgcat tacaatgatt tagatataga
    18601 aaccaacacc tttgaaaata tattaatgga tgaaaattat gatgagacaa atgaagtttc
    18661 aataggtaat atgtttttta aaataaagac catatacatc aaagatgatg aatttaaggt
    18721 ttgcaaaata gtacaagata taagtgattt aaagagaaaa gaagaagaat tagttttgaa
    18781 aacagtggct ataagagaag ctcatcatag agtaaaaaat aatcttcata cagtaatatc
    18841 tataataaaa aaacaaagta gattatctca aaatgatgaa gtaaagcagt gtttagataa
    18901 cataataaat agggtatttg caatactttc gactcattat ttactatcta aagaagttga
    18961 taataatata tctatacgag aaggtatcaa tttattagtg tcaaatattc aaggtggcta
    19021 tttatttagt aaggatatag atatacgtat aactggtgaa gattttaaaa ttagtggaga
    19081 aaaagcaaca gcaatacttt tggttataaa tgaaatacta caaaattgtt atgaccatgc
    19141 attttcggat agagaacatg gtactataca aatattaata aataaagata acgactatag
    19201 aaatattgct attatagatg atggaattgg atttgatagt aataatataa ataaaaatag
    19261 cttagggaca tatataatag atagttatat aacacagatg ttaaaaggaa atatagagag
    19321 aaaaagtagt gagaatggta cagaagtatt aattactata cctacacaaa ttaatattta
    19381 aataataact catctacaaa gtagtagttt taattgagca aagaggctta aggttattaa
    19441 tagattaagt taattgaatg tatttataaa aattataagt atatattcaa tttaacaggt
    19501 atttattata actttaagcc tctttgcttg tttaagacat acttaaattt taggaggtga
    19561 gaaaatgaga gaagagttat taagtgttgg tattgacatt ggaacaagta ctactcagtt
    19621 aattttttct aaattaatag tagaaaatac tgaatcaaat ttttcggtac ctagaataag
    19681 tatagtagac aaagaaatta tttataaaag caaagtttat ttcacccctc ttatttcaaa
    19741 taatgaaata aatggtcaac aaataaaaga tatagttgaa aatgaatata aaaaagcaaa
    19801 tatatctaaa gaagatatac atacaggagc agtaataata actggtgaaa cagctagaaa
    19861 agaaaatgca aatgatgtac ttcatacttt gagtggattt gctggggact tcgttgttgc
    19921 aacagcagga cctgatttag aaagtataat atcaggaaaa ggagctggag ctcatattta
    19981 ttcaaaagaa cattcaacta caattgtaaa tatagacata ggtgggggaa ctagtaacat
    20041 agcctcattt aaaaggggag aagtaaccga tactgggtgt ttggatatag gtggaaggct
    20101 tgtaaagata gataaaataa gtaaaaaaat aacttatata tcacctaaaa tagataaaat
    20161 cataaaagat aataatttaa atcttcaagt aggtacaatg gctactgtag aaaatacaaa
    20221 gccaatagtg gatataatgg ttgacttatt aatggaaagc ataggactaa agaaacagac
    20281 acctttgttt gattttataa taaccaataa atcaataagt atagatgaaa atattaaaaa
    20341 tgtttcattc tctggtggag tagctgatta tatttactat gatggagaaa taaaagatta
    20401 ttttgaatat ggtgacttgg gaattttact tgggcaagca ataaaaaatt gtgaatacct
    20461 taaaaaagta aatgtagtta aatctatgga aacaataaga gccacagtgg ttggagcagg
    20521 ttctcatact actgaagtaa gtggaagtac aataacttac actagcgaaa agtttccact
    20581 taagaacttg ccagttttaa aattatcaat tgatgaagaa acaggtactt caaaacaaat
    20641 cagtgaagct ataaaaaata aacttaattg gtttaaacta gaaaatgatt tacagaaagt
    20701 tgctatagcc atgataggaa agaaaaatcc aaccttttta gatatacaag agtatgctaa
    20761 agggtttgta gatggaatgc aacaaatcat agaaaatgat ttacctttta tagtaattgt
    20821 tgaaaatgat atggcaaaag tgttaggtca agcaatatat gcattattaa attatgaaaa
    20881 agaaattgtt tgtatagaca gtgtcaaggt tgaaaatgga gactatatcg acattggaaa
    20941 acctatagtt gaaggtaaag ttttacctgt ggtaataaaa acattagttt ttaattaata
    21001 gaaggaggta agtaaatgat tttaaataca aaattatttg gacgtactta tcagtttaaa
    21061 ggcttatatg atgtaatggc taaagctaat gaagaaaaat caggagataa attagcaggt
    21121 cttgcagcag aatcagctga agaaagaata gcagcaaaag ttgttttatc acacataaaa
    21181 ttggaggata taagaaataa tcctgcagtg gcatatgaag aagatgaagt aacaagaata
    21241 atacaagatg ctgtaaatga aaaagtttat gaagaaataa aaaattggac agtgtctgaa
    21301 cttagagaat ggctattgga ttcaaatact acaagtcttg atataagaag aatatcaaga
    21361 ggattaactt ctgaaatgat agcagcagtt gcaaaattaa tgtcaaatat ggacttagtt
    21421 tatgcagcta aaaagataaa ggttactgct cactgtaata caactatagg agaacaagga
    21481 acattatcag caagacttca acctaatcat ccaacagacg acccagatgg aatacttgca
    21541 tcacttcttg aaggtttaac ttttggtata ggagatgcag tacttggatt aaatccagtt
    21601 gatgactctg ttgagagtgt tactaaagta ttaaaaagat ttgaagaagt aaaacaaaaa
    21661 tatcaaatac caactcaaac atgtgtattg gcacacgtta ctacacaaat ggaagcaata
    21721 agaaatggtg ctcctgcaga tttaatattc caatctatag caggttctga aaaaggaaat
    21781 gaagcatttg gatttaatgg cgacactata gaagaagcta gacagttagc cttaaaacac
    21841 ggtacagcta caggacctaa cgttatgtat tttgaaactg gacaaggttc agaattatct
    21901 tcagaagcac atcatggaat agaccaagtt actatggaag ctagatgcta tggatttgct
    21961 aaaaagtatg aaccattctt agtaaataca gttgttgggt ttataggacc tgagtactta
    22021 tatgatgcta gagaagttgt aagagcagga cttgaagacc actttatggg taaactacat
    22081 ggcttaccaa tgggtgttga tgtttgttac actaaccata tgaaggctga ccaaaatgat
    22141 atggaaaact tagccatact attaacaaca gcaggatgta cgtattttat gggaatacct
    22201 catggagatg atgttatgct taactatcaa acaacaggat accatgaaac agcatcactt
    22261 agagaaatgt ttggactaac agcaataaaa gaatttgatg cttggatgga aaagatggga
    22321 ttctctaaag atggtaagtt aacagagttg gcgggagacg catctgtatt attaggatag
    22381 gaggtattta atatgaatga gaaggattta aaagcattgg tagaacaatt agttggacaa
    22441 atgcttggag aattagatac taatgttgtg tctgagactg ttaaaaaggc aactgaagtt
    22501 gtagtagaca acaatgcttg tatagatgat ataacagaag ttgatataag aaagcaatta
    22561 ctagtaaaaa atcctaagga tgcagaagct tatttagata tgaaagcaaa aacacctgca
    22621 agattaggta taggaagagc tggtactaga tataaaacag aaacagtttt aagatttaga
    22681 gcagaccatg ctgctgcaca agatgctgta ttctcttatg tagatgaaga atttataaaa
    22741 gaaaataata tgtttgcagt ggaaacttta tgtaaagata aagatgaata cttaacaaga
    22801 ccagatttgg gaagaaaatt ttctccagaa actataaata atataaaatc taaatttggt
    22861 acaaatcaaa aggttttaat attagttgga gatgggctta gctcagctgc aatagaggca
    22921 aatttaaaag actgtgtgcc agctataaaa cagggtctaa aaatgtatgg tatagattca
    22981 agtgaaattt tatttgttaa acattgtaga gttggtgcta tggaccactt aggtgaagaa
    23041 ttaggttgtg aagttatatg tatgttggtt ggagaaagac caggattagt tactgctgaa
    23101 tcaatgtcag catatatagc ttataagcca tatataggaa tggcagaagc aaaaagaaca
    23161 gttatatcaa acattcacaa aggtggaaca actgcagttg aagcaggtgc tcatatagct
    23221 gaacttataa aaactatgtt agataaaaaa gcttcaggaa tagaccttaa ataaagtcga
    23281 gggagtgtag aatatgaaaa atgatgtaat tcgtcctaat atactaggaa ttaaaataat
    23341 ttcaaatata agtccagaaa tggcacaaaa actagaatta aaatcacatc acaaaagctt
    23401 aggtttaata actgcagact gtgatgatgt tacgtataca gctcttgacg aagcaactaa
    23461 agcttcagaa gttgatgttg tatatgcaaa atcaatgtat gcaggtgctg caaatgcttc
    23521 aacaaaactt gctggtgaag tattaggaat aatagcaggt ccaagcccag cagaagtaag
    23581 aagtggtcta aatgcagtag ttgattttct agagtatgga gcaactttta taagtgcaaa
    23641 tgatgatgat tcaatagcat actatgctca ctgcgtatca agaacaggta catacctttc
    23701 agaggtagca ggaataagag aaggtgaagc attagcttat ttagtagcac caccacttga
    23761 agcaatgtat gcactagatg cagcaatgaa agctgcagat gttaagatgt gtgaattatt
    23821 tgcacctcca actgaaacaa actttggtgg agcattactt acaggttctc aatcggcttg
    23881 taaagcagca tgtgatgcat ttgcagaagc agtaaaatca gttgcagaca atccaacagg
    23941 attttaagga tagttagatg gatgctatag gcttaataga agtaatagga tatgttgctg
    24001 caatagaagc ttctgatgct tgtttaaaat ctgcaaatgt taatattgta agagttgata
    24061 aggttggggc aggtattgtt actttaacaa tatgtggaga tgtcggtgct gtaaaagctg
    24121 ctttagaagc gggtgaaata gcagcttcca gagttggtac attaagaaca tcacatatta
    24181 taccgagaat ccataatgaa gtaacaaatg ctttattcaa aactaaagaa cctaaagtct
    24241 gtgaagtttc agaagataaa gaaaaaaact ttgagattac attggaaata gctgataatg
    24301 tatcgaaaaa tttatcagaa aaagaaaatg taattttaga tgtcaatgaa aacagtggag
    24361 atactgaaaa tttatatagt gatatagata aaacaaacaa aaaaattgaa acagttgatg
    24421 atagtttaac agaagctaat gaggaaaaaa tagaaaacac taatgataaa gaagtattgg
    24481 aacaagttaa aatagaaaat tccgattttg atagagatga cctatcaaag aacagtgtaa
    24541 aaaaacttaa agctatggct aaaaaattag attcttcaat aacttataaa gacttaaatg
    24601 ttcttaaaaa agaagaatta ataaacttag ttaaaaagct tagcagaagg gatagatagt
    24661 tatgacactt ttagataaag atttagtatc tatacaagaa gtaagaactc ttgtaagaag
    24721 atctaaagtg gctcaagaga aactttctaa aatgagtcaa gagcaaatag ataaaatagt
    24781 aaaagctgtt gtagatgcag catttaataa tgctactaaa ttagctaaaa tggcaaatga
    24841 agaaactgga tttggaaaat gggaagataa agtaattaaa aatacttttg catctatggg
    24901 agtttatgaa actttaaaga acacaaaaac tattggaata attggtgaag ataaagaagc
    24961 caatacgata gatatagcta caccagttgg agttgttgca ggtcttatac cttcaacaaa
    25021 cccaacatca acagtaatat acaaaactat aatatctcta aaagcaggaa atactatagt
    25081 tttctctcca catccaaatg caaaaaactg catacttgaa acagttaata taataagtga
    25141 ggctgctata aaggctggag ctccagagga ttcaatatta tgtataacta atccaagtat
    25201 agttggaacc aatgaattga tgaaacataa agatacaaac ttaatactag caactggagg
    25261 agaagcaatg gttagagctg cttactcttc aggaacacca gctattggag ttggaccagg
    25321 aaatggacca gcatttatag ataaatcagc aaatgttaaa ttagcaatca aaagaataat
    25381 agattctaaa acttttgata atggtgttat atgtgcatca gaacaatcta tagtaataga
    25441 aaaatctatg gaagaaactg ttttaaaaga gcttaaaaat caaggtgcat atgttttaaa
    25501 tgaagaggaa agtgaaagat tagcaaaatt catattaaga gctaatggta ctatgaatcc
    25561 acaaatagtt ggtaaaagtg ttgaggtaat agcaaattta gcagacttaa caaatgtacc
    25621 taaaaataca aaagttctag ttgcaagaga aaataaagtt ggtcacaatg tagcctattc
    25681 aagagaaaaa cttacaccaa tactagcgtt ctatgttgaa gaaaatgtag aagctattat
    25741 gaacagatgc agagaaatat tgttaaatga aggtgcaggt catacattct caatgcatgt
    25801 aaatgatgaa gaattggtaa aaagatttgc ccttaatatg ccagtatcaa gaatacttgt
    25861 gaatactcct tcagcacttg gaggaatagg tgcatcaaca aaccttgtac cagcactaac
    25921 acttggttgt ggtgcaatag gtggaagttc aacttcaaac aatataggac cacttgattt
    25981 aataaatgtt aaaagagtag cttatggaat caaagaaata gaagaacttg gaagagataa
    26041 atctcaagtt gaaaaatcaa tgtttgactc aatggataaa gaagaattaa taaatcttct
    26101 tgttcaaaaa ctagtcaaag aactagcata actaaaataa acaatgaaac ttatggctat
    26161 cgctataaat ttctaaaaat ataaactata aaaaactata aaattaaatt tttggaggat
    26221 aagattatgg caagtgcaaa cgcattagga atgatagaaa ctaaaggatt agtaggagca
    26281 atagaagcag cagatgcaat ggttaaagct gcaaatgtac aattagtagg aaaagagcaa
    26341 gtaggtggag gattagttac tgtaatggta agaggagatg ttggagctgt taaagctgct
    26401 acagatgcag gagctacagc tgccgagaga gttggagaac taatatcagt tcatgttata
    26461 ccaagaccac actttgaagt agatgctata ttaccaaagg tatcagctga gtaatataag
    26521 aataattata aaatttaaat tttgattgaa aaagaggcta tctcaaaaat gagtcttact
    26581 aataaactca atgtattttg aggtagtctt ttttatacat ctaataggag atgtgataaa
    26641 atgggtgttt taacagaaaa cgaagtaaaa agaaaaattg gaaaaaacaa aaataatata
    26701 actgaatttt atatagaaaa aggtcaaata ataactcctt ctgcaaaatc ttatttagct
    26761 gagagaagta ttgagcttaa atatgttgat aaactttcag atgtaaacaa tgaacctaaa
    26821 gaagttgtaa aagaagtagt taaagagaaa aaatataaat atactactgt atttggcgcc
    26881 aaattggatg aaaaaccaga acatatgact caattaaatg gaaatctttt agtttttaaa
    26941 gaccataaaa gaataatatt gagaggaaaa atagattctc ttgaatcaga aatactaaag
    27001 gtacagatac tttgtcagaa actagatatg ccaaagcttg ttaaggaatt gcaagaagta
    27061 ttagactttg taagaaatat aatgagatgt gaagttttag atgaaaagct aggggacttt
    27121 aaacttttag gattaactcc tcaagaactt agagaacaat ctcactatcc taaaaaatac
    27181 tttggtatag gacatgagtt cccaagttat gaaatgggag aagtagtagt agctataaat
    27241 agtataagaa gtaatacacg agaggttgaa cttgcagcct atgaagcatt taagggtgag
    27301 tatggaaatg ttcacagaga agatttaata aaggcactta atagattatc gtcagtattt
    27361 tggataatga tatataaggt tagaactaat aagtataagt agttggagga cataatggag
    27421 aatctattaa atgaaatagt ggaagaagta ataactagag ttaaaaaaga agcatttata
    27481 gaagttgaag catcaggaag acacgttcac ttaagtagag aagatgtcga taaattattt
    27541 ggagaaggat atactcttac aaaactaaaa gacctatctc aaccaggtca atatgcatgt
    27601 aaggaaagag ttacaataac tggtcctaaa ggaagtataa aaaatgtagt tgtacttgga
    27661 ccatgtagaa atgaaactca ggtagaaata tctttaacag atggttcaac tcttggacta
    27721 aaagctccta taaaacaaag tggagactta gaaggtactt taagtattaa aatatcaaca
    27781 caatatggtg aagttgaact tgataagggg ctaatggttg ctaaaagaca tatacacatg
    27841 actcctaaag atgctgaaaa attcaatgta tgtgataaag agatagttga agctaaggtg
    27901 atgggtcaaa gacctttaac atttgatgat gtagtaataa gagtgagtga cagttttaaa
    27961 acttatatgc atatagacta tgatgaagcc aatgcatgtg gttattcaaa aggaactgtt
    28021 gctaaaataa taaagaaagc ataaatttaa cctactatat aaattacctc tagggaggat
    28081 ttaatatgaa ttatgataac cttgtagatt taataacgga agaaatctat aaaaaaataa
    28141 atagtaatga aataaaaata tctaataaac ctaaagctgt gattgtattt gaacaagata
    28201 acaataaatt taacttatta aaagatggat ttgaaatagt agaatttgat aaaagtataa
    28261 gggaatgtga aatagtgata gtttcaagac tttgtatgag ggggctatcg aatatagcac
    28321 ttggaaattc aacaagtgat gaagaaagat ttatactaaa aatgattatg aatggcaaaa
    28381 aagtatatgt cctagatgaa ggaattgaat acaaaaaata taaggatact gcaccaaaag
    28441 cactatataa gaaatttatg tcatttgaag atgaaatatg taaatttggt gtagaaatta
    28501 taaaagactt aaatgttata actaaaaata agttaaatat aaagaaagaa ctagaaagta
    28561 ctagaggttc taaaaaagaa gatacaaaag ttttagataa agatatttta aatttaacca
    28621 gtgtagaaag tgtaaaaata gataatgaat ttagtttgga tttaaggaat aaaaaactta
    28681 tatcggaagc tgacttaaga aaaccaacta taaatggagt taaaaacata ttagttaata
    28741 aaaaaagtat aataacacca ttagctgtag attttatgag aatacaccat ctcaaactaa
    28801 agaaactgta aggaggtaat tttatggata taggaaaagt tgtaggtaat gtttgggcta
    28861 caagaaaaga tgaaaaatta tgtggccaaa aacttttagt tgtaaaaatt ttaaaaacta
    28921 aagacacata caaggaaggc ttcatggttg cagccgataa tgcaggagca ggaaatggag
    28981 acttagttct tatatccaaa ggttcctcag ctagagagtc gattgaaaaa tctcatgttc
    29041 cagtagatgc aactatagtt ggagtagtcg attcgtttga ggtgtttgat gaataaaagt
    29101 ataggagcta tagagtttaa aagcattgct aaaggtatag aagtatccaa tgaaatgata
    29161 aagaaatctt cagttgatgt attgtactta aaaagtattt gcccaggtaa atttctaata
    29221 atagtaggtg gagaaacttc ttatataaat gaatgtgtag attatggaat aaaacttgga
    29281 gaaggatata ttgtagataa cttcgtaata aatgcaattt cacaagaaat attagatgga
    29341 tttaaaaata agtatcaaaa gctagatagt atagtctcta taggcgttgt tgaaagtagt
    29401 aaaatatgcg ctggaataaa aatgcttgat aagactttaa aatctggcga cttagtatta
    29461 gtaaaacttc aattatcttt tgctataggt ggcaaattag tatatatagt agcaggagat
    29521 ttaagtagtt tagaatatgc attaaaagaa agtgaaaatg tagtaagaga aaaagaagta
    29581 atatataaga ctgtaatacc atcagtagac agtcaaataa taaaaagtct aataaaataa
    29641 tggggggaat tatgagtata aacgaaatta taatctatgt aatggtagtc ttcatggtac
    29701 ttggagctat agataaatgt ataggtaaca aatttggtct tggagaacaa tttgaggaag
    29761 gtataatggc aatggggtca cttgcagttg caatggttgg tgttatatgt ttagcaccag
    29821 ttcttgctga tgtattaaga ccaattgtag taccagtatt taatatactt ggagctgacc
    29881 cagctatgtt tgctggaagt ttattagcta atgatatggg tggtgcgcca cttgcacttg
    29941 aacttgcgaa agaccctaat gctgggttat ttggtggatt aatagtaggg gctatgatgg
    30001 gacctacaat agtatttata ataccagttg cgcttggaat aattgaaaaa gaagaccaaa
    30061 aattcttagc aacaggaata cttgcaggta taataacaat acctattgga gcattcgttg
    30121 gtggattagt tgcaggattc gaggtaatga tggttcttag aaacttagtg ccaataataa
    30181 tatttgcagt agttatagca cttggattat taaaattcga aaatgctatg ataaaaggat
    30241 tcacatactt tggaaaaggt gtagtaatag ttataacttt aggacttgct gctgccatag
    30301 ttgaagcttt aacaggaata gttgtaatac ctggaatgaa accaatcagt gaaggtatag
    30361 aaatagttgg agatatagca atagttttag caggagcatt cccattagtg tttgttataa
    30421 caaaggtatt taataaacca ttaatggctt taggaaaagt tcttggaatg aatgatgtat
    30481 cagcagcagg ccttgttgca agtttagcta actgtatacc aatgtttggt atgatgaaag
    30541 atatggacaa tagaggaaaa ataataaatg tagcattctc tgtatcagca gcattcgtat
    30601 ttggagacca cttaggattt acggcaggat ttaattctaa tatgataaca ccaatgatag
    30661 ttgctaaatt agttggtggt ataacagcag tattgttagc tatggttatc gcaaataaga
    30721 cactaaagaa ggagaatgta taagatggat atatcaaata tagataaaaa tttaattgaa
    30781 acattagtaa gacaaataat agaagaaaaa ataagtggaa ctaaagacac agtagatttt
    30841 gttagaaata aagacattag tggaataact tctataaagt taccaactgt aaaagttagt
    30901 gaatctgata gattagatac agggaatcca agtgatgtag tttacacaaa agatttattt
    30961 acattagagg aaagcccaag attaggttgt ggtatgatgg aaatgaaaga aacaactttt
    31021 gattggactc taaattatga tgaaatagat tatgtaatag atggaacttt agatataata
    31081 atagatggaa gaaaggtttc tgcttcttca ggtgaattaa tattcatacc aaaaggaagt
    31141 aagatacaat tttctgtacc tgattatgct agatttatat atgtaacata tccggcagat
    31201 tgggcatctc aaaattaata aaaatcaagt taagttaata atcaaataaa taatttaaaa
    31261 tacaaactct taaataagca cgcatattta aaaaagctat gaattaaaat tcatagcttt
    31321 ttttataaag aatgttatat tttagacgta ttttttctat atttactggg agatatatta
    31381 tattttttat taaataatct aataaaatgc tctacatttg gataaccaat actattttac
    31441 tgataatctt ttccttatca ctaatcttac attttaaaat attattttta ctcgtaatat
    31501 tatcataaga atttgtttct tatttaaaac taattagaat tataaagttt attgccatgt
    31561 tattaataaa ataaatccag ttaaatcgag tgctagttaa agtaataaga aacttttaca
    31621 atggtttcct ttatcgattg aaataaaagt taatatggta taatatctaa agagagaatc
    31681 ttatcaatta gaatatgcat agaaacaagt ggcttaaaag gagacgttat atggaagaga
    31741 tagtaaagaa ggaacaagca agtatgaatg atattttttt gtatcttaaa gctaaatcaa
    31801 actcattata taaatttgta atattatata atgagtttat caataaacct aaagattatg
    31861 gaactgggga acttataaac atggttgaag tacatatgct aacagcgatt gaagaaaatc
    31921 caggaataag tccaactcag ttagctaaaa tatggaacag aactaaaggt gcaatatctc
    31981 aaactatatc taaattggag aaaaagggtt atataactaa agaaaagaaa gaaggaaatg
    32041 caaaaactat attactatat ccaaccacat caggagtaga gttaagtaag gcacataaac
    32101 tatatgacac catagatgtt acaagaacat ttgaatactt aatcaaggag tgtacattag
    32161 aggagataga tagtttttat aaagtaattg atttgtttat taatttatta gaagaatctc
    32221 cgcaggaaaa taatgattct gaaaaagtgt agctgagatt gaagttgaat aaagagaaat
    32281 agatattata gaagtagtac ttaagtacac att
//
LOCUS       DBGJFU010000036        30496 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.036, whole genome shotgun sequence.
ACCESSION   DBGJFU010000036 DBGJFU010000000
VERSION     DBGJFU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.036"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(77..436)
                     /locus_tag="ACOIAS_001603"
     CDS             complement(77..436)
                     /locus_tag="ACOIAS_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HGZ2701317.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(490..1575)
                     /locus_tag="ACOIAS_001604"
     CDS             complement(490..1575)
                     /locus_tag="ACOIAS_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HGZ2701318.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(1746..3125)
                     /locus_tag="ACOIAS_001605"
     CDS             complement(1746..3125)
                     /locus_tag="ACOIAS_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HGZ2701319.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(3118..3798)
                     /locus_tag="ACOIAS_001606"
     CDS             complement(3118..3798)
                     /locus_tag="ACOIAS_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HGZ2701320.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4190..5269)
                     /locus_tag="ACOIAS_001607"
     CDS             complement(4190..5269)
                     /locus_tag="ACOIAS_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2701321.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      5557..5672
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5847..7217
                     /locus_tag="ACOIAS_001608"
     CDS             5847..7217
                     /locus_tag="ACOIAS_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2701322.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFVGLILTVFFMRK
                     TDKSIS"
     gene            complement(7483..8094)
                     /gene="yihA"
                     /locus_tag="ACOIAS_001609"
     CDS             complement(7483..8094)
                     /gene="yihA"
                     /locus_tag="ACOIAS_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGZ2701323.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8084..10447)
                     /gene="lon"
                     /locus_tag="ACOIAS_001610"
     CDS             complement(8084..10447)
                     /gene="lon"
                     /locus_tag="ACOIAS_001610"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGZ2701324.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            10627..11139
                     /locus_tag="ACOIAS_001611"
     CDS             10627..11139
                     /locus_tag="ACOIAS_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HGZ2701325.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11127..11654
                     /locus_tag="ACOIAS_001612"
     CDS             11127..11654
                     /locus_tag="ACOIAS_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HGZ2701326.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(11725..12975)
                     /gene="clpX"
                     /locus_tag="ACOIAS_001613"
     CDS             complement(11725..12975)
                     /gene="clpX"
                     /locus_tag="ACOIAS_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HGZ2701327.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(12994..13578)
                     /gene="clpP"
                     /locus_tag="ACOIAS_001614"
     CDS             complement(12994..13578)
                     /gene="clpP"
                     /locus_tag="ACOIAS_001614"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGZ2701328.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(13728..15014)
                     /gene="tig"
                     /locus_tag="ACOIAS_001615"
     CDS             complement(13728..15014)
                     /gene="tig"
                     /locus_tag="ACOIAS_001615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGZ2701329.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(15208..15678)
                     /locus_tag="ACOIAS_001616"
     CDS             complement(15208..15678)
                     /locus_tag="ACOIAS_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2701330.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(15678..17027)
                     /gene="rph"
                     /locus_tag="ACOIAS_001617"
     CDS             complement(15678..17027)
                     /gene="rph"
                     /locus_tag="ACOIAS_001617"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGZ2701331.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKIIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(17187..19211)
                     /gene="ligA"
                     /locus_tag="ACOIAS_001618"
     CDS             complement(17187..19211)
                     /gene="ligA"
                     /locus_tag="ACOIAS_001618"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGZ2701332.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(19397..20335)
                     /locus_tag="ACOIAS_001619"
     CDS             complement(19397..20335)
                     /locus_tag="ACOIAS_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGZ2701333.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(20437..20859)
                     /locus_tag="ACOIAS_001620"
     CDS             complement(20437..20859)
                     /locus_tag="ACOIAS_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HGZ2701334.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21047..22330
                     /locus_tag="ACOIAS_001621"
     CDS             21047..22330
                     /locus_tag="ACOIAS_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HGZ2701335.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(22408..22923)
                     /locus_tag="ACOIAS_001622"
     CDS             complement(22408..22923)
                     /locus_tag="ACOIAS_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2701336.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNLDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(22923..24299)
                     /locus_tag="ACOIAS_001623"
     CDS             complement(22923..24299)
                     /locus_tag="ACOIAS_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HGZ2701337.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(24324..24875)
                     /locus_tag="ACOIAS_001624"
     CDS             complement(24324..24875)
                     /locus_tag="ACOIAS_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2701338.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRQCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(24933..25106)
                     /locus_tag="ACOIAS_001625"
     CDS             complement(24933..25106)
                     /locus_tag="ACOIAS_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701339.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            complement(25117..25950)
                     /gene="fdhD"
                     /locus_tag="ACOIAS_001626"
     CDS             complement(25117..25950)
                     /gene="fdhD"
                     /locus_tag="ACOIAS_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGZ2701340.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(25999..28143)
                     /locus_tag="ACOIAS_001627"
     CDS             complement(25999..28143)
                     /locus_tag="ACOIAS_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(27727..27729),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HGZ2701341.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(28394..29452)
                     /locus_tag="ACOIAS_001628"
     CDS             complement(28394..29452)
                     /locus_tag="ACOIAS_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HGZ2701342.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(29663..30322)
                     /gene="cprR"
                     /locus_tag="ACOIAS_001629"
     CDS             complement(29663..30322)
                     /gene="cprR"
                     /locus_tag="ACOIAS_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HGZ2701343.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT         9204 a         5526 c         3303 g        12463 t
ORIGIN      
        1 tagaattttt ttaattatag ccatctcctt cttaaataaa aataatttta aaacttacag
       61 caatcaatat aaaatattat tttggataaa attgcttaga atttatattt acgcttatat
      121 ctgttccgct atcatttatg acaagttcga atttacctga tgtactctga ggaactggaa
      181 cagtatttag gtatcccttt tccttcaatg tctctacact tagagagtct attgtctttt
      241 catcatcatt aattgccatg ctagctgcgg ttactatatt agcagcattc gtatagtctg
      301 catttaattt ggcactttct tgaaccttac tatacttaac taatgctata ctagatataa
      361 cgcctaaaat agttactact acaatcattt caacaagtgt aaatcctttt cgttttttat
      421 ttatcaaatt tatcaattaa atattctctc cttttcaaaa gtttcatcta ataaaattta
      481 tttttctctc taaaaattag taattgcatc aaacatagga acaaccattg ctaaaataaa
      541 agtaccaata attaatccca taaatacaat tagcataggt tctataaatt tcattatttg
      601 ctctattttg ttatttaatt cattttcata aaatttattt attacattta gacttttatc
      661 tagtttccca ctttcttctc ctgtatttgc cattgaaata aataattttg gaaaaacatt
      721 tgctaaattc aaagatttac ctatgctatt tccttttttt atataattat tggaaatctt
      781 taacctttca taaataaatt cgttgtctat aacactagct gacatgtcta tagcttccac
      841 aatttgtatt ccactgctaa ttaaaacgga caatgccctt gaaaatcttg ttgtagctac
      901 taactgatta atttttttta taaaaggtat tttgaatttt aacgaattaa tcaatttttt
      961 tatattatca tttgttataa agaaataaat gcttcctatt gaaaacaata taaaactaaa
     1021 tattatataa gctaagttat ttgtaaccac tgtagatatt cccattaaaa ctctagttat
     1081 taaaggtggt tcaattccat tgttggtaaa aaccacctga aaattaggta ttacaaatac
     1141 gaatataaac aacattgata agatagataa tattataagt attattggat atattgaaat
     1201 agttattatt ttcattttta atttgtattc tttatcataa taattagata aatcactcat
     1261 tattatatct aagtttccac tagtttctcc tgcttttatc atacttataa agaatttaga
     1321 aaatatacct gttatttgaa atgattctgc tattgagtta cctttttgaa tatgatttga
     1381 tactatacta agcaaattct ttgctttttt acttgactga ctctcaatga tttcaaatat
     1441 ttttgtaatt tcacatccag attccagcaa tatgcctatc tctttgcaaa ttactctaat
     1501 ttctttatca ctgattctat ttaatactaa ataaaacttt tctaaattat aaaatatatt
     1561 tttagataat ctcatgtact ataatacctt ccaaattata ttaattacaa ttaaataaac
     1621 ttaatatttt ctaatttaat gcatttaatt ttatttcact taaatataat ttatactcaa
     1681 acatcttaat taaaatagat taaataaaga caactgacca aacgaagttt aactatacaa
     1741 ttcagttaaa tcatttcatt tactactaaa cactcttcta atgtcgttat tttttcattt
     1801 attagtcttt tacaactatc ttcaaatgtt atcattccat tattttgtgc tacatcttta
     1861 atagtactag aatcctccat atttcttata catgtcttta tatcatcatt aatttctaag
     1921 atttcataaa ttgctgttct tccaaagtat cctttatcat tacatttttc acaccctaca
     1981 gctaccttag tatttacatc tccactaaaa ttatctttta tcataatctc atggctacaa
     2041 tgattgcaga cttttctaac caacttttga gatataacac ctataagaga agcatttaaa
     2101 agatatggtg gtatttgcat ttctagtaat ctagctatgg aagatactgc atcattagta
     2161 tgtaaagtac ttataactaa atgacctgtt atagctgctc ttactgctat cttagctgtt
     2221 tctgcatctc ttatttctcc gaccattatt atatctgggt cttgtcttaa tatcgctcta
     2281 agacctacat caaagctcaa ccctactttt gaatttactt gaatctgact tatgccatcc
     2341 atcttatact ctacaggatt ttctatagtc ataatatttt tatttattcc ttttaaatca
     2401 tttaataagg aatatactgt tgtagtcttt ccactacctg taggacctgt cactaataat
     2461 attcctgctc ttttatttat tatacgattt atagatttta tagcttcctc tgaaaaacca
     2521 agttctaatt tgtcttttaa aaaagtatct ctattaagta tcctcaaaac tattttttca
     2581 ccatagacag ttggtattga tgaaactcta atgtcaatta aatttccacc taactttata
     2641 tccaccctac catcctgagg aagccttttt tctgttatat ccattgatgc ctttaatttt
     2701 ataaccgtag acaatgatgg atagacatca actaaaaact ttgatacttc ctttagttct
     2761 ccatctaccc tcattctcac tattaaatgt tctttaaatg gttctatatg tatatcactt
     2821 gcgttttctt ttatagcttt ttctaaaaga ttttcaaata atttttttgc ataatcctca
     2881 tcaagttcaa agctattata atatttttca atcttttcac ttataagttc ttttttctcc
     2941 ttcttcaata taatttcttt acctgtagct aatctcaagt cctgtagagc ataaatatcc
     3001 tcatcttcta tttctacaac taaattatca tcttgaaact caactggaaa tacattatat
     3061 ttttttgcaa gtttttctgg tattaacatt ggcaaactgc tttcctctaa tataatctta
     3121 atcacccttt atatacttta atttattagg tatattcatt tttttcatag agatattttc
     3181 ttcagttttt atgcttattt tcattggata attggtttgt atgctatcta aatatttact
     3241 acctttatct acataattag atagtttctc cttatctcca atgcacttta tttcatatgg
     3301 ttgtgctaca ggaactccat ttatatttat atggcttcct gcaagtacaa cttctgaata
     3361 ttgattaatt ctttgaccat ttatttctat gtattttcct ccgtttactt ttaattcatt
     3421 tataactttt acaagaatct tattatagtc tatactatta gctatattcc ctgttttttc
     3481 atctatagca tctatactta atattatccc actacccttt acatctgaat atgatagact
     3541 atctctaagt gtttctattt ctgatatttc ttcagaattt ttttgatttt gtttcattga
     3601 attaatttct ttatctaact tgtctttctc tttggttaat tcttttatag attttcttgt
     3661 catttttatt tcttttctta ttgacttgtc tttgcttgta taaactgttt cttcattgct
     3721 ataatctcta aatatggttc caacaagtat acctaaaata gtacttgcta gtattataaa
     3781 cacatattgt tttttcatac tgtattgctc cttacaaatt tatttttata tgataatttt
     3841 ctttaattaa atattaaaca tttacatatg cttattatat caaagttata tattaaaatt
     3901 catctttagt atataatatc atacattgaa ctcctatcac taatttacca atatcaagta
     3961 tgcattttta gatgttctta aaattatata aactttcaat aaacattgtt ttgatattat
     4021 aaaaaaacat aaattaggaa aggaaataat aattatgaaa aacaaataat tgctttggag
     4081 attattaata tcatttttta gcaaaaaaaa gtaaaatggc atagcctcaa agctacacca
     4141 atttactaaa aattatatca ttattaacat taattttaaa catatacgtt tataagtttt
     4201 cctcatattt taaaattctt ttaattaatt tatctcttaa tttccctact tcatcaaatc
     4261 tcatattagg actatagtaa ctttctataa tatcatgttt agacttggct ctcttaagat
     4321 ttagtactgc ccaacaaatt aaatcatcta aaactttttc atcaaattta atatcttctt
     4381 catatttaag tagtttttct ctatttaaaa actgatttaa atcaattgta tcttcttctt
     4441 taaaagtcgt acttatagtt atgcctatat ctaaatcagt taatagtaca gtttcaattt
     4501 tctctggaat aagtggataa tgataaactt ctacatttaa acctttttca atagctttct
     4561 tatatacttt gtttaataat gtagttttcc cagttcctat ttctccatct aaataataga
     4621 ctttctttac atcttttaaa atgttttctg tataatctaa acaccctaca ggagttatag
     4681 ctgaaccaaa taaatgcttt tctcttttta gatttcctgt gcttttaact ccatcaaata
     4741 acctttttat aaattcttct gtgaccaaat ttactttgcc ataatccata caatcagaat
     4801 atttttcttc tatgtctcta aagattggtt ctgctgattt taaaaactta aatgctcttt
     4861 taaatgaatt actaatatct ttatcataaa ctgatatttc ttgtttattc ttttctaatt
     4921 cttctgtatt ccagtaatca cctaaattaa ttatctcatc caaagctcca ggtagctttg
     4981 ggtccatagt atgtggggca gttgcatcca taagtaccac gcctaatttc tttataacta
     5041 aaccatccaa cgaacttggg tcagaaggac aatgatataa atcaatatca tatcctctat
     5101 gcaaaaactc tttataaatt tttttcataa gtgaggattt tcctactcca ggtccaccct
     5161 ttaaacaaaa aactcttttt acattttcag gaattatata gtcaaaaaaa gaatatgagc
     5221 cttgtgatgt atttccaccc gggaatgcct tctttacctt tccttccata ttagcgcctc
     5281 ctcataattt acaatatctc ttaattaaga tattcatatt ataagaattt tattacagct
     5341 ttctgatttt gacaaattta aaacttgcca tctaatacta ataacaggtt aattttatct
     5401 aattattata gattctcata ctgtgcctta ttctatctat aaatacaatt taagtgtcca
     5461 tattgaaata tttgtattgt aatacagctg gatattactt aaatccaatt gtttccatta
     5521 taattttatg ttaaaataat attacaaaat acatctgttt ttcttcataa acgggtgaaa
     5581 ttacctatcg gcggtaaaag cccgcgagcc ttatggcata atttggtcat attccaaagc
     5641 cgacagtaaa atctggatgg tagaagaaaa tagtatatga gtacctttat gtaattttac
     5701 atgagtaatc tatacaaatc cttcaactac cgtatttatt catgaaatta gacacattca
     5761 aggtacctaa tatacaggtg ctttttttgt tgtttatttt acaattatat cgtacttata
     5821 aaatctatta agattggagt gttatcatga aacaaaaatg gatagtattg attatcatct
     5881 gtattggtgt atttatgtct actcttgatg gaagtatact aaatatcgca aatcctacaa
     5941 ttgctgctga ctttaaaatt aacatgtcac aaattcagtg ggttgtaaca gcttatatgc
     6001 ttgttgtaac agctactatg ttatttttcg gaaaacttgg cgataaagta ggttcaaatc
     6061 gtctatacac attaggattt tttatattta caataggttc ttttctgtgt agtatgtcaa
     6121 acaatttatc aacattaatt tcttcaagaa tctttcaggc agttggagca agtattttaa
     6181 tggctactgg gcttggtata gtttccaatg catttccagc aaatgaaaaa ggaaaagcta
     6241 taggaatcac tggagctgta gttggtatag gaaatatgag tggtcctgta attggaggta
     6301 taattttaga acattttgga tggccttcta tttttattat caatatccct attggtatta
     6361 tagcagtctt tcttggcatt aaatttctgc ctaaacctgt tttggatgaa caaaacaaga
     6421 gctttgatat tccaggtctt ctacttttcg catcttgcac tacgcttata ttacttgcaa
     6481 tgaatgagaa gggtaataca agattgtatt taggtataac agcacttata atcttcttat
     6541 tgttggcatt aagagaagta aaatttgaac aaagctttat tgacctccct ctatttaaaa
     6601 atagaaactt tactgtaggc aatatcattg gagtagcttg ttattttcct caaatggcag
     6661 tatcttttct acttccattt tatttagaac aattaaaaaa tttatctcct atgatggctg
     6721 gttatgtaat gactgtacat ccacttatta tggtacttat agcacctatt gcaggttcac
     6781 tttctgataa acacggtgca aaaaacattc taacagcatc tttttcattt atgaccatct
     6841 cactagttgg aatggcactt ttaaaagcag attctccatt gtacctttta attgtatgtt
     6901 tagttatttt tggacttggg ctaggagcat ttagttcacc aaacaatagt agtattttag
     6961 cagatgttcc tcctcaaaaa caggggtacg gaggcagttt tcttgcaact attcgtaatt
     7021 tatcatttgc tcttggaaca gcatttttta gtagtttttt tgcacaaagt cttacataca
     7081 atcaaaagtt taaaagtcac acatcagcct atgttatagc tagcaatcag tcttattgga
     7141 ttgcagcatc agtatgtttt gtagggctta tactaactgt tttctttatg aggaaaacag
     7201 ataaatctat ttcttaaatg ctatttatta ctgactacat aaatcaataa ttttttagtt
     7261 taactatttt atactaattt tcccatacct tactaaaatt tattagtatt tagcaaaatt
     7321 actgatattt attgtttata aacaataagg atgccaaaaa aaagttcatt aaaactattt
     7381 taaggcatcc tatattttac tttaatatta atataattaa aactaactat tattacattt
     7441 attattcaaa taaatcttat gtcaatattt aacattttaa atttaatcta ctgttatttc
     7501 atatccatgt tgattgtatt gatttacaat ctcttcccat aattcttcaa caccagtctt
     7561 ttttagtgaa gaaacaggaa ttactttttc actactttcc atttgcaatt tttttcttat
     7621 tatacttatg tgtttttgat attgtcctct agatatttta tctgccttag ttgcaataac
     7681 tacacagtta tatccaaagt gctttatcca ttcatacata agcttatcat ctgctgtagg
     7741 ctcatgtcta atatctacaa gtaggaatat agaacataac tcttgtctac tttctagata
     7801 tcgttccatt atacctcccc acttttcttt ttcagactta gctatctttg catatccata
     7861 gcctggaagg tctacaaaat aaaattcatt atttattaga taaaaattta tagttctagt
     7921 ttttcctggt gtttgactgg ttctagcaaa gtttcttctg ttaagcaatg tatttattat
     7981 tgatgatttt ccaacatttg acctaccagc caatgctatc tctgggattc cttctgctgg
     8041 atactgtgat ttattcacag cactcatagt tatctctgaa cttctaattt tcattatttt
     8101 caccactctt taataaagct tgttctaata cttcatccat atgttctaca agcacaaact
     8161 ccattttttc ttttacattt tctggtattt catctaaatc agcttcacat tctttaggaa
     8221 ttaatacttt tgttatacca gctctatgag ctgctaataa cttttcttta actcctccta
     8281 ctgctaagac tctacctctt aaagttatct ctcctgtcat tgctatattt ccaggcacag
     8341 gtcttttagt aagtgctgag attactgcaa gagccatagt tataccagct gatggtccat
     8401 cttttggaac agcaccttct ggaatgtgta tatgaatatc attcgtctta taaaattcag
     8461 ggtctatatc aaacttgtcc actattgacc ttatataaga gatacctgtc ttcgcagatt
     8521 ccttcataac atctcctaat tttccagtta atacaatttc gcctttacct ttaagtacgt
     8581 ttacttctac ttcaagtgta actccaccaa cttctgtcca cgcaagacca tttactaatc
     8641 ctatttgagg gttaacttct gcaagttgat acttaaacat gtcttttcca agatatgtct
     8701 ctaggtcaga cttatttata acaacctctt caagagttgg gtcttcaacg tattttttgg
     8761 caacctttct acaaatctta ccaatagttc tttctagtgt tcttacacca gcttctcttg
     8821 tataatggtt gattatactt cttaaagttt catcatctat ttttataaag ttttctttta
     8881 aagcatgttc tttaatttgt tttggaagta ggtatttttt tgctatattt aatttttctt
     8941 cttctatata tccagatact tctattactt ccattctatc taataatggt cttggtatat
     9001 tacttagact atttgctgtt gtaacaaata gtatttttga taaatcaaaa ggtatttcta
     9061 agtaatggtc cacaaaatcc ttattttgct ctgggtctaa tacttctagc attgctgaag
     9121 ctgggtcacc tttataatct gctgccatct tatctatctc atcaaataag aatacaggat
     9181 tttttgtttg ggcttctttt accccattaa ttattcttcc tggaattgaa cctacataag
     9241 ttcttctatg acctcttatt tctgcttcat ctctaactcc acccaaagat attcttacaa
     9301 attttctatt taatgctctg gcgattgatt ttactataga tgtctttcct gttcctggag
     9361 gaccaactag acatattata ggccctttaa gtgacttagc aagagttctt atagctagat
     9421 actctagtat tctctcctta actttttcta atccataatg gtcttcatct aatatatctt
     9481 ttgccttagt tatatctaat ttatcttttg tctctttatt ccatggcaat gagaatatag
     9541 tatctaaata gtttctactt acagatacat ctggagacat agaagatatt tttgaaaatt
     9601 tatctatctc tttttctatt ttctctttag ttgtcttagg tgcttttatt tttttaagct
     9661 tttctctata ctcatctgct tcagagttta tatcttcttc ttcacctaat tctttctgta
     9721 tagcttttaa ttgttctcta agataatatt ctttttgaac cttattcatc tgttttttta
     9781 ctcttaaagt tattttcttt tctattttta atatatctat ttcctctaat aatatagaat
     9841 aaataagttc aagtcttttt ctaatatcaa attcttctaa tatttcttgt ttttggcttg
     9901 attttagata tatgtttgca gctatcgtat ctataaatct atctacatct tctatatcag
     9961 ctaaagatat taatatttct ggtgataccc tatttccaat atttatatac tcttcaaagg
    10021 catcaaatac atttcttaca aatgcttcaa tttcaacttc tgtttcacta tccaaattat
    10081 cactatcaaa tactatttct tctattactg ctctgaaata cccatcttct tgttctattt
    10141 tctttactct accccttgat acaccttcaa ccaaaacacg aactgtatct cctggtaatt
    10201 ttatcatttg cttaacttta cagattgttc ccacatgata aaaatcctct tctcctggct
    10261 catctacctc tgcttctttc tgagaagtta aaaatataag ttcttcatcc atcatagctt
    10321 ggtctaaagc tttaagagat atctctctac ctatatcaaa atttaatatc atatatggaa
    10381 atatagctaa tcctcttaga ggaattaatg gtaattcatg atctatttta gtataatttt
    10441 gttccatcaa cattactcct tttctagtct aattgctttg cttttaatat tatatatttg
    10501 aacaagttaa ttttcaacat taatgataaa ttcttagaat actatcatac tatattatct
    10561 ttttattatg atataatatt aatattagta tacttagatg ttatttatct tttggaggtt
    10621 tctattatgt tgaaattgtt tttaaccttt ttaaaaattg gtgcgtttac atttggtggt
    10681 ggatacgcta tggttcctct tatacaagat gaacttgtaa acaaacaaaa acttttagag
    10741 caggaagaat ttattgatta tttatccata gctcaaagtt atcctggagt actggcagtt
    10801 aatatatctg tattattagg atataaactt tatggtatac caggagttct tatatgtacc
    10861 ttaggctctg ctttaccatc attttgtata gtcttagtct tatcgtatct atactttaaa
    10921 aattcatcat ctaaaatttt agatggattt tttaaaggag ttgcgcctgt tgtaatagct
    10981 cttatattgt attcttttac gagcatgttc aagaaactac caaagtctaa aactaacata
    11041 ctactattaa ttatagcagt tttatctgtt ggtgtattca atataagccc tatttattta
    11101 ataattggag gaggtgtata tagtctatgt cagaaataat actactcttc ttaatattcc
    11161 ttaaaatagg ggcatttagt tttggtggcg gttatgccat gctaccattt atccagcaag
    11221 aatttatatc taaatatcac tttataacta atcaagagtt tttggattta cttgctatat
    11281 cacaatctac accaggtcct gtagcaataa atagtgctac atttgtagga tttaagactg
    11341 ctggcttctt tggttctcta gccgcaacta ttggtgttac aatttttgca ttaataggtc
    11401 taactattat atcaaagtta ttttctaaat ttaaaaataa taaaaaggta gaaaacttat
    11461 tgacagttct tagaccaatt actctaggat tcatattatc agcagctttt tcatctgcca
    11521 aagaagttaa ttgggattta tatggaataa ttgcatttat agcatcattt tatcttttat
    11581 ataaaggaaa aattggttcc attgcaattg tattcgtctt tggtattcta ggtatattac
    11641 tcacattttt ataataatga tacctatttt atatttagca tattaacata aaaaaaggag
    11701 cttttaaagc tccttttcat catcttatgc actttcctct tgatccttaa gtactatgat
    11761 aggatttact gagtcttcgt tcacagattt ctctgttact ataaccttct ttatattatc
    11821 tctagatggt acttcaaaca tagtttccat cattacgctt tcaactatac ttctaagtcc
    11881 tctagctcct gtatttcttt ctatggcctt tttagcgatt gcacgaagag caccttcttc
    11941 aaattctagc tcaacatcat ctaactccaa taacttttta tactgtttta ctagagcatt
    12001 tttaggttcc tgtaatatct gcattaatgc atcttcatcc aacaactcta aagtagctaa
    12061 aactggtatt cttcctataa actctggtat tataccatat tttaataaat cctctggaag
    12121 tactttttca tatagcttcc ctaaatctaa ttctttttta ctttctattt tagctccaaa
    12181 acctagtgtt ttatctccac cacgtttttg aattatcttc tctaatccat caaaagctcc
    12241 acctaatata aataatacat tagtagtatc tatctttaaa aattcttgat gaggatgttt
    12301 tcttcctcct tgtggtggaa catttgcaac agtaccttct aatattttta gaagtgcttg
    12361 ttgaacacct tcaccactaa catctctagt tatagatggg ttttcagatt ttcttgcaat
    12421 cttatctatc tcatctatat atattatacc tctttcagct ttttctatat caaaatcagc
    12481 agcttgaata agttttaaaa gtatattttc aacgtcttct ccaacatatc cagcttcagt
    12541 caatgatgta gcatctgcca ttgcaaaagg tacatttaga gttcttgcta gagtttgagc
    12601 aagtaatgtc tttccagaac ctgtaggtcc taataaaagt atattactct tttgaatttc
    12661 tatatcctta ctactagatt ttttactgta tattctctta taatgattat aaaccgcaac
    12721 tgataaagct tttttagctt tttcttgacc tattacataa tcatttagaa tctccatcat
    12781 ttcctttggt tttggtaaag atgtagtatc ctcatctata gtatcttcta tctcttcttg
    12841 aataatttcg tcacaaagtt caacacactc gtcacaaata tatacatttg gacctgcaat
    12901 taatcttctt acttgatctt gatttttacc gcagaatgaa cactttaatt gtcgtttttc
    12961 ttcatattta gacatattaa cacctctctc tatctacggt ctctttgtaa atacttcatc
    13021 tattaaacca tattctttgg cttctagtgc actcatgaag ttatcacgtt cagtatccat
    13081 tttaattttt tctaatggct gaccagttct ttcagataat atctcattta aagtttcttt
    13141 tatttttaaa attctctttg catgaatttc tatatctgtt gcttgacctt gagctccacc
    13201 taatggttga tgtatcatta tttcactatt tggtagtgct agtctttttc cttttgctcc
    13261 tgctgccaac aagaatgctc ccatagaagc agccatacct atacatatag tacttacatc
    13321 tggtttgatg tattgcatag tatcatatat agccattcca gaagttatac ttcctcctgg
    13381 agaatttata tataaatgta tatctttgtc tgggtcttca gcttctaaaa ataatagctg
    13441 tgctactata agccctgctg tggcatcgtt aacttgatct cctaagaaga ttattctgtc
    13501 ttttaatagt ctagaaaaaa tatcataaga tctttctcct cttcctgttt gttcaacaac
    13561 tacaggtact aatgccatgg tttcataccc cctttattct atataattta cagtagcttt
    13621 ttttaagcta ctgtaaatta ttatacgcat ttaactaaat tatatttgca catcattata
    13681 tactaaacat ttcataacgt caactatatt agttagcgtt tattttatta agctattgtt
    13741 gcattatcaa ctaataaatc tattgttttt cttattttta tttgaccttt tatatcttct
    13801 ttgtcggcat ctcttaaaga agcttctatt ttttctactt ccatattgta agcagaagcc
    13861 attttttcta actcagcctt aaattcttct tctgttgctt caattccttc agctttagtt
    13921 attgcttcta atactaaaga agacttaact aattttttag catcttcttt tctctcttct
    13981 cttaattctt ccatagttct accagtcatc tctagtaatt gttgtaatcc aaatccttga
    14041 tattgtaatt ggtagttaag ttcatttaac atattatcta tttgatgttg tatcataact
    14101 tctggtattt ctatttctgt attttcagca actttttcta ctactgaatt tctagtttct
    14161 gcatctgctc tatttttagc ttcttcttct aacttagctc ttacatctgc ttttaattca
    14221 tctaaagaat caaattcact agtatcttta gcaaattcat catcaagtgc agataattct
    14281 tttactttta catcatttat ttttacatta aatacaactg gttttccagc taaatcttgt
    14341 gagtgatatt cttctgggaa agtaacatta acttctactt cttcaccagc ttttttacct
    14401 actaattgtt cttcaaatcc tggtataaat gtgtttgaac ctataactaa gttataattt
    14461 tcacctttac caccatcaaa agctacacca ttttcaaaac cttcgaagtc tattatagct
    14521 gtatctccat cttctaaagc tttgtcttct acacttacta atctggcatt tttttcaacc
    14581 atttcttgta gtttagcatc aacattttcg tcacttacag tattctcaac cttagctatt
    14641 tctataccct tgtagtttcc taattcaaac tctggtttaa cttctacatt aagaaccata
    14701 accaacccat tgtctttact tatttcttct atatctaaat ctggattgtc tattgggtct
    14761 atgtctaatt ccttgatagc ttctggataa acttctggga ataatatttc tatagcatca
    14821 ttgtagaata taccttttcc gtattgagtc tctataacaa ttcttggagc tttacctttt
    14881 ctaaatccag gtatattgta tttatttctg cttttattat aagccttatt tactgctgct
    14941 tcaaatttat tgttatctac tgttattttt aaagtaactt tattaccctc ttttttaatt
    15001 agttctgctt tcatccttct aatcctccta gtgatattta tgtaataccc gataacggta
    15061 ttataattca ttctatttat aattcaacca cttaattata tcacataagt ttttttattt
    15121 acaagtgttc cattaatatc gttgtactta tttgataaaa tttaattttt tgagcgtttt
    15181 ttgccatttt tttagataaa tacaccttta tatgcgtatt tcttcaattt ttatgtcatc
    15241 ttcttctata tccataatga caaaactttt atacgatact cctcttggaa gtgaagtgct
    15301 tccaggattt atgtaaagta caccatcttt aatttctcta aatggggtat gggtatggcc
    15361 aaatataact atatcagcat caacttcagt tgcctttttt tctaacatat gagttccata
    15421 ttttacgcca tacttatctc catggcataa gaatatggtt ttatttgcca cttcaaagac
    15481 aacttcttcc tcaacagcat caaagtcaca attaccttta actgcaataa tacctacatt
    15541 agtcatgcta tgaatatatc ttgaatcagt aaagttatct cctgcatgaa ttattaggtc
    15601 acattctttc aaatatggta ttgttttttc catattttta atcatcatat gtgtgtcgct
    15661 tactatgcct attttcatta cttcactacc ctttttataa actcttgttt cataagtttt
    15721 agtgcgtttg ctctatgact gattgaattt tttataacac taggtatttc accaaaagtc
    15781 ttatcatatc cttttactat aaataagcta tcatatccaa aaccattttt acctttttct
    15841 tcaaatccta tcattccttc acaaattcct cttactacaa attcttttcc atcaggaaaa
    15901 actactgcta ttgcagagac gaatcttgca tttcttttgc tcattggtat attttgcata
    15961 gcttttaaca gtttttttct attttcttca tctgttgcat gttctcctgc atatctagct
    16021 gaataaacac caggtttttt gcctaaagca tctacttcta agccagaatc atctgatatt
    16081 gctataagtt tagtcttttt tgctattgtt ctagctttta tcaacgcatt atgctcaaat
    16141 gtttttccat cttctactat ctctatacct gctaaatcaa catcttttaa agagtacact
    16201 ttatattcaa aatcttttaa gatttcacct atttcttcta gcttatgagc attattagta
    16261 gcaatcacta cttcattacc ataatccata cccaatatct catctgatat ttcacctaaa
    16321 gcttctcttt gaagccttat taattcttta ttcccttttt cacctaactc taacaataaa
    16381 tttaaatctg ctctcgaaaa aggtttctct tctcctgtcc cctgtacttc tacaaactca
    16441 cacttatcag tcattattat attcatatct acctgtgctt tagaatcttc ttcataacat
    16501 aaatctagca tgtgtattcc atttacaata ccaacactaa ttgcagacac aaaatttgtt
    16561 attggcatct cttttattat ctttgcttta tataatttat atattgcttc tgcaactgct
    16621 acaaatgccc ctgttataga tgcagttcta gttccaccat ctgcctgaat cacatcacaa
    16681 tctacccaaa tagttctttc tcctaattta ttcaagtcta caacagacct tattgctctt
    16741 cctattaacc tctgtatttc ttgtgttctt ccatcaattt ttcccctaga tgcatctctt
    16801 atctttcttt gttgtgttgc tcttggtagc atagaatatt cagcatttat ccatccagta
    16861 ccactatttc ttaaaaaagg aggaactttt tcttccactg atgcagtgca tataaccttt
    16921 gtctctccca tttctataag aacagaacct tctgcatact ttgtataatc tctagttatc
    16981 tttacaggtc ttatctgttc gttacttcta tcataacttc tggtcatttt ttatatcctt
    17041 tctcatattt tttatttttt ataaatttat ttttataatt tttaattttg tcaaatatta
    17101 attattgtca tttttcgtaa tcttattagc ttactgaact attctatcat taattatagt
    17161 agttttctac taaattaagc gactttttaa ctctctaata cagcttttac ttcatctttc
    17221 gatgataact ttaataattc taaaaatctc tcttcatcta taactttaat tcctaaatca
    17281 tttgctttag taagtttaga accagcttct tcacctgcca aaacaaatgt tgtcgattta
    17341 ctaacactag atgtagcttt tccacctctt ttttctatca tatcttttgc atcatttctt
    17401 tttaatgtag gaagtgtacc agtcaatacg attttcatcc cttcaaatat tttttctact
    17461 ttttcacctg tattatccaa tgattttgta tttactccta cattttttaa cttgtttata
    17521 acgttcatat ttttgtcttc cttaaaaaac tcgacaacgc tgtttgccat tatacttcca
    17581 aattcctcta attcttctag ttgctcacta gttgcagaaa ttacttcatc taaatcctta
    17641 aagttatatg caagtatctt agctgcttta actcctataa atttaatacc taatccattt
    17701 ataagtctcc ataaatcatt ttcttttgat ttttcaattg aatttattaa attgtcagca
    17761 gatttttcgc ccattctctc taggtcgact acatcttcct tttttatata atataaatca
    17821 gatacatctt gaataatttt ttcatttaat agcagtgtaa ttatagattc tcccaatcca
    17881 tctatattca ttgcatccct tgacacaaag tgtataattc cccttcttat ttgggctggg
    17941 caagacatat tgatacattt tacagcagct tctccttcta atcttattgt tggctctgaa
    18001 caaactggac acttttctgg cattttaaaa tctatttcat ctccagtacg ttcttctttt
    18061 ataacttcta aaacttgagg tattatgtcc cctgcttttt gaactaatac tgtatcattt
    18121 atttttatat ctttttctct tatataatct tcattgtgaa gtgtagctct acttacagta
    18181 gtacctgcaa gtctgactgg ttcaagaatt gctgaaggtg ttattgtacc agttctccca
    18241 acttctacta ttatatctac tattttagtt ttcttctttt ctgctgggaa tttatatgca
    18301 attgcccatc taggactttt ggctgtatat cccatttcgt ctctttgttt tatattgttt
    18361 acttttatta ccataccatc tatttcgtag tctaaattac ccctgttatc tgtccagtat
    18421 tcaatatgct caataacttc atctatactt ttacaaactt tataactctg acttactgaa
    18481 aatcctagtt tttctaagta ctctagtgat tcactgtgac tttctaaatc gtattcttct
    18541 atattttcca tattaaatac aaatatatct aatggtctct ttgctgttag ttttgggtct
    18601 aattgtctca atgaaccagc tgcaagattc cttggattag caaataatgg ctgttcttgt
    18661 tcttcttgtt gttcatttac ttttctaaaa ttatcttttg atatatatac ttctcctcta
    18721 actattagtt cttttttatc atttatctta agaggtatgc tttttactgt cattaagttt
    18781 tgagaaatat cttctcctac aactccatct cctctagttg ctcctttttc aaactctcca
    18841 ttgttataag ttagacctac agaaagacca tctattttaa attcaacaac atattcaaca
    18901 ttttctccca catattcttg aactctctta tcaaaatctc tcaaatcttg ctctgaaaaa
    18961 gcatttgata aactcaacat aggagtttta tgtacaattt gattgaactt atctaatggc
    19021 ttacctccta ccctatttgt tggagaatca attctcttta gttctggatg ttcttcttct
    19081 aaagaaatta actccttcat taacttatcg tattcataat ctgatatttc tgggctatct
    19141 tgattataat acctttcatt atggtagttt attgtctcta taagtttatt aatttcatct
    19201 tttacgctca ctttttgcac ctcttcatat ttttaaaatt aaattatttt gaaatttccc
    19261 tataattaat tatctcctat ttatatattt tttagtatat cactttttgg gctttaaatt
    19321 tattacaaaa aaaactctta ttagtatctt taatacgact aataagagtt tttattttta
    19381 tcaaaatttt ttatttttaa tagcctcttg atatgtctac aacattatct acaggtttat
    19441 tctccattat atagtgtttc aaattattat aaaccatatt aaatgttctc tctttgtttt
    19501 tatctgacac ccaagaatta tgaggagtta ctatgacatt ttcgcactcc cacaacttat
    19561 tgtccttgtt taaaggttca ctttcaaata cgtctaatgc tacccctcta aatttctcta
    19621 cacattcttc taaatctttc tgattaactg tatttcctct acctacattt ataaatacag
    19681 aaccatcttt cataagttta aacatatctt tgtttactat tccatctgta ctttttgtag
    19741 aaggcattgc tacaactact atatcacatt cttcaaatac aatattcata tcttcagtag
    19801 caaaacactt atcaaagtat tttttactac ttccatcagt attgacaccc catatttcta
    19861 caccaaaagc ttttaatctt tttgctgctt ctgaagatat agtacctgtt cctaaaaatc
    19921 caactctttg tccatacaat tctgatacag aaaaatccat cttccattct ttattttgtt
    19981 gttgtttaaa aaattttgca ctatttttat atatttctaa aatatacaat acaatacttt
    20041 caccaatagg tacactatat ccgcctctat tattagctat cttaatattt ctcttcaata
    20101 cctcttcttt tggaagttgg tctataccta cactagttgt ctgtatatat tgtaaatttt
    20161 tcatcttaga aatatctaat ctctcaaatg gattgtatgt aactagtaca tctgctgtat
    20221 ttacatcctc attattttca atactatctt cagagtaatg tatgacatca tagcctaatt
    20281 ctcttatttt atcaaatttt tcttttccat aatttattgt aaataaaact ttcatctcaa
    20341 atctcctcca tctacccaca tttaaaattt tatattctaa aatataatat gatactcaat
    20401 gtacattagt taaaattact ataagatatc ttaaatttac ttatgttcaa ttgtttttcc
    20461 taaagacata gcattacgtt cttgaactaa attttctatt aaattattca atgtccaatc
    20521 aatagatgtc tctgttacca tagctgtaaa aggtctattt attctcactt tttttagccc
    20581 ttctaaaaat gcatttactt gtgtgcttgg agtgttatta aatattacag acttactctt
    20641 aatcccattt tcacattcat ttgatatatt tccatctaaa atgtctctta ttatattctc
    20701 tccattttta tcacttaaga tgatatggtc tcttattcca gcaaagttac ttacgttttt
    20761 aattaatgat aattcttttt tattaaagtt tacaagcata atacacttcc tgttgtgtat
    20821 tttatctagg ttattattat ttattttttc aaatgacatt ttatccatcc tttcatttat
    20881 ttatatactt taatttaccc catacattta aaataatcaa tttttatagt taatttttat
    20941 attaataccc aaattatatt taaatagttg ggctatccaa aactaatata taaaattaga
    21001 ataattagat taatatctac aaatcataac ctaaaggaga gataccatga atacattact
    21061 aaacaataaa tttagcatta aaaacttttc aaatgcttgg gttatagtac ttggttgtat
    21121 gttgatacaa gctgttccat ttagtattgt agcaagtata cagcctcaat ttataagtta
    21181 tgttgtagaa ggagaaggct ttagtttagc ttctttttct ctaatattca ctattggtac
    21241 tattgcctca gcaattgcat caccttttat aggtgtatta ttttctaaag tcaatattaa
    21301 gatactatat ttattaggtt gtataatttc tagtattgct tttgctactt tttctatatg
    21361 tactgaacta tggcaatttt acactgtatc atgtattgtt caaattggcg ctacaattat
    21421 atctgctata ggaatacctg ttttaataag tgcttggttt gatgaagaaa gcaaaggaaa
    21481 agctttgggc tttgcttttg ctggtggttc tattggtagt atatttctac aaccacttgc
    21541 agttcgctta ttagcaactc aaggatacag tcgttcttat ctaatatttg gaattatagc
    21601 attaatagtt ggtatacctg taatattatt catactcagg atgcctaaag atattaatga
    21661 aaaggtaaaa tctaaaggta aaaaagagaa agcatctgat acaatttctc cttcagtatg
    21721 gggttataca ttaaaagaag ctaaaaaaat taaatatttt tggattatga ctattggatt
    21781 tatatttgtt ggtatatatg tttcatcatt atccgtacaa tatccaactt atcttagaaa
    21841 ttatctaaat cctattctaa taggaacagt aggctccaca tttgctttat ttgctctcat
    21901 tggtaattta tttggaggat tattgtttga taaattcggt caaacaaagc cacttattat
    21961 atcttttgta ttggttctta tagcaacttt atcattacta ttggtagata aatcttctac
    22021 ttttgcattt gtatttgcca ttttgaaagg tctttctgta tttgcttata tgattggtcc
    22081 tgcttattta actggctctt tctttggtca aaaggagttt ggtagcatat atggaatcgt
    22141 acaattaatt tttgcagtgg gatttgcatt aggttcaaca ttatttggat tgttagttga
    22201 taattttgga tacagatttt cttggtctat tataatttta tcagttataa ttgcctatac
    22261 tttacttatt ataacttcta taggaatgaa aaaattgaat aaagaaagat tagataaact
    22321 aatgcaataa ttgattttta gatatataaa tattataata aaaaagatgg agcttttaat
    22381 cataaataaa actccatctt ttttatatta caatatagct aattttctct tatttttaac
    22441 tttactatct atatcctcat ccttaactat agtaaatgcc ttagttggac aaacatttac
    22501 acatgcaggc ccttctggcg attctataca aaaatcacac ttatttgctg tcattctttc
    22561 tctaaagcaa agctttcctt catctacatc ttttaatgta actatgtcta ttgcacctat
    22621 aggacatgct aacatacaag tcttacatcc tatacaattt tcttcattta tttttattgt
    22681 gttcccaacc ctaactatac caccatttgg acatgccttt gcacaaggag catcttcaca
    22741 atgtctacac tgtattggag cacttacttc ttttgtcttt atgacagaaa gttttggatt
    22801 aaaatttatt tcatctagat tttggtaaaa aatatcttca tctgaatgtg caactacaca
    22861 tcctatcata caagttctac atcctatgca tttatcaggg tctgctataa caaaatagtt
    22921 catcacttag ccacttcctt tcctatacaa tatgtagtgt gtaatagatg atgagatttt
    22981 tctcctaatg gttctaccag atattcatca tacagttttt taatatcagg attctcatga
    23041 gattttctaa ttgctaaatg ttcatcatgc acgtatgttg cttttgttct attttcataa
    23101 gccaattcct tatattcttc tagtaaaatt tttggttgac ctccaccact tacacaccca
    23161 acaggacaag tcataacttc aataaaatct acatctaatt ttcctttttc caagtcttca
    23221 agcactggta ttacattttt taatccactt actactccaa ctcttaagtc taagtctcct
    23281 attttaatag ttgactttct aaaaccattt tctcctctta cttgttttac ttctacatca
    23341 ggaatacttt ctccagttat aagctcatat cctgttctta tagcagcttc cataacccca
    23401 ccagtaacac caaagatagt tccagctcca ctataactac ctaaaggact atcaaatttt
    23461 tcttctctta aatttttaaa atcaattccc atgtctttta ttaaataagc tagttctcta
    23521 gtagttaata ctacatctac atctctatat ccactagaat ccatttcttc tctatcacat
    23581 tcatattttt tacatgtaca tggcattata gaaacactaa atatctttga tgcatctaca
    23641 ttatttatct ctgcaccata agttttaaat attgcaccac ccatctgctg aggtgattta
    23701 caagaagata aatgattagt taatttagga taattttttt ccatgaattt tacccaagct
    23761 ggacaacaag atgtaaacat tggaagagtt ccaccttctg tcactctttt aattaattca
    23821 ctaccttctt ccattatagt taaatctgct gcaaaattag tatcgtagac tctatcaaaa
    23881 tttaaagctc taagtgctgc tgccattttc ccagcaccta agtatcctaa ataatatccg
    23941 aaatcttctc ctaaagcaac tctaactgct ggtgcacatt ggaccatagt gaatacattt
    24001 gggtcactta aagctttctt caccttatct atatcacata cattgtatgc tgcaaatata
    24061 ggttcattta tagactctgg tatctctctt tcttgttttt tctcttgtaa aaactcaaat
    24121 ccttcatcta taactgaagc ataagattta caagtttgaa cacattgacc gcacataaca
    24181 catctatcta aatttatctc ttgtggcttt ccctcttcac cttcaatagc atttactgga
    24241 cagacctttg aacattctct acatccagta cataaatcct tatctatatt tattatcata
    24301 aacgtcacct acccctgtat cctttattta tttgttaaca ataaactaag tgcagcttgt
    24361 ctattcttag cttttttgtc atctattgga gtaacaagtt ttaatgcttc ctgaggacat
    24421 gcacttatac aagctatttt ttcattgtct ttacataagt cacatttata agctatcttc
    24481 ttattttcat ctaaaattac ttgttctact tctttaccat cttcatattg tggaagtaag
    24541 tctatagctc caaaaggaca tgctagcaga caagttttac atcctataca cttttcttca
    24601 tttacagaca taatattgtt ttctttaact atagcttttt gtggacatga gtttaaacaa
    24661 ggagcatctt cacactgtct acactgtatt ggcatacaga aattgtcacc ttttactaag
    24721 taaagtctag gtataacagg tatatcaact gttccaactg tataacccac attattatgc
    24781 tgattatgca ttgtaaaaca agctacttca caagctctac aacctgtaca tttgtttggg
    24841 tctgctatta caaaagagcc tattttattt ttcatattat cccacctttc aatttgtata
    24901 tttccaaccc tctagttatt ttcatcatat gtctacttac tttcatcata tatatttata
    24961 tatttcacta ttttctcagt acttatattt cgtttacatt ttttgcacaa attgtatgtg
    25021 ttattataca ccttatcagt atcatcaatt aatactccac agatttcaca ctttgaatat
    25081 ctttgctctt cttttttagt acacatatat tcattctcac tttttgataa ctctctgtgg
    25141 atttgcataa atattcatcc gtcttcctct tacaaatcca ataagcgtta tatttaattt
    25201 tttagaaagc tctattgata aattagtcgg tgctgatttt gatactacaa taggaatttg
    25261 agtcatagct gcttttaata tcatttccaa tgatattctt ccacttacag ccacaatttt
    25321 atcttttaat gttatgtcat ttaaaacaca aaatccaata gccttatcca ttgcattatg
    25381 ccttgctaca tcttcacaag tgactatttt tttatcaaaa ttaaatacag atacgctatg
    25441 taccccacca gtatttttaa ataattctga tgaatttaaa tttctatcca ttatactgta
    25501 aatcgtatca taatttattt ttatattgct ctttacagga gcacatgtta tatagtctag
    25561 tgaatttaaa aatataattt gctcatcttg atttttttgt ttctcttgaa tagttacttt
    25621 tgcaaaacgt tcactctcat ttatctcaaa ttttaataag ctttttttgt tttctatgta
    25681 tccttttgtt ttcaagaatc ctactataag ttcctctaaa ttatatgggg tacataaaaa
    25741 tgtatttata tatttatcat ttaaaataaa actaagtgga tattctgcaa ttatttcttc
    25801 attatctatt gatacttcat gttcatttat tttgcttact tctacattaa aagaatttaa
    25861 atatttgtag tccactagac tataactatt ttcaaaaact ttatccttac tattttcttg
    25921 taatttagtt tttgagtata tattgttcat aaaatattag cctttctgcc ttttataaat
    25981 cttaaatcta ataaatcatt agatatttgt agctgtcatt ttgtctttca aactttgata
    26041 ctcttctctt acacattttt ctgctaattc ttggtcttca agtttttcaa gtttaacagc
    26101 acaatattta tactctggtg ttttagatat aggatctaaa tttgctattg taagttcatt
    26161 gcaagctcca acccaccact gataagtcat atatgttgca ccttccttaa ctctatcagt
    26221 aactgttgct cttgttatta cacttcctct tcttgaacta attctaacta attcatcatc
    26281 ttcaattcct aatttttcag catcattaga atttatttga acacgtcctg gttcatcttc
    26341 taatgaacta agtgttctac aattaccagt cattgttctt actgaatagt gtccaacttc
    26401 tcttacagta cataaactaa atgggtattc atcatcttca acttccatag ggggtctcca
    26461 ttcagcagcg aacaaattac ctttaccatt aggagtagaa aacttttgac cttcatacag
    26521 atacatagtt cctttatctt ccatactttc actcttacaa ggccattgaa cgcatccttg
    26581 agtttctatc ttttcataag tagcacctaa gaaacttgga cataattgtc taagttcgtc
    26641 ccatatttcc ttagtatttt tataattcat aggatatccc attgctgtag aaatttcgct
    26701 tataatttgc cagtctggtt ttacatctcc ttgtggctct atagcttttc tcatcaattg
    26761 gaatcctcta tctgcacatg tatatactcc ctcatgttct ccccaagaag tagctggaag
    26821 tataacatca gcgtgaagtg ctgtcttatt cataaatata tcttgaacaa tgacaaattc
    26881 caattcatct aatgcttctc taacttctga tgcatctggg tcactttgca ctgggtcttc
    26941 tccaaatata taataagctt taagcttttt ctcttttagt actaaatttg gaacttgagt
    27001 taaactataa ccattattag gagataattt taccccccaa gctttttcaa acttttctct
    27061 tatcttatca tcagttacat tttgatagcc aggatataca ttaggaagag ctcccatatc
    27121 acaagctcct tgaacattat tttgtccacg tacaggtcct attccaacgc tttctctacc
    27181 aaaattacct gtaagtagag caattgatgc aagtccctta actacatcaa cagcttgacc
    27241 aaattgacag acacccattc cataaagtat catagctttt ttaccttttg catattctct
    27301 catagctttt cttataagct cttctgggat tcctgtaatc ttttcagcat atttagctgt
    27361 atatggttta acaatttctt tatactcatc aaatccttgt gtatgatttt gaacaaattc
    27421 tttattgtaa agaccttctt ctattaatac atttccaaaa gcatttacta aaaccatgtt
    27481 tgttccacct tttataggta gatgtatatc agcaattctt gcagattcag ttactctagg
    27541 gtcagtaaca attaatttag caccattttt tttagctttt actattctat ttgccactat
    27601 tggatgtgaa tctgctccat tatatccaaa tataaataaa acatctgcat tttctatctc
    27661 tggtatagaa ttagacattg caccactacc taatgagtaa gccagaccgg ctacagatgg
    27721 tgcatgtcag acacgagcac aatggtcaac attattagtt cctattgtag ctctcataaa
    27781 tttttgcatt atatagtttg cttcattacc aggtcctctt gcagaacctg ttcccataat
    27841 agaatcagga ccatattttt ctttaatttc atttagtctt gaagctgtat agtttatagc
    27901 ctcatcccat tcaacctctt ctagtacacc ttgttttctt atcattggct tttttagcct
    27961 aggtgttaga attttagggt catttagaaa atcccatcca taacgacctt ttaaacacaa
    28021 actttcttga tttgttctac cattagccgg ttcagctctt aatatcttat tatcttttac
    28081 tactagatat aattggcatc cagctccaca gtatggacaa actgttaaaa tttttttctc
    28141 catacaattc cccccttaga tttatcccct gataagtaaa atttatctta aagataatga
    28201 gtgtttttat aatatgcaaa atcataaata ttttgcaata ctatctatta aattcaacaa
    28261 ttttctcaca attcctctta aatttcaaaa aaaatatgtc ataataatct ttatatttca
    28321 accatttaat taaaatattc tagttcttat gacatatttt ttaatatact caatatatat
    28381 tctttaaact tatttagttt tcacttaaaa caatatttct gccaattcta tttttcattc
    28441 tatagtgagt aattacagta aatattccac ttccaatcag aatcaaatag tacataaatc
    28501 caccatataa aaatactgca tatcccatca aaggttgagg gaatactgat ttgaatatag
    28561 tgaaaaaggc taattcatta gctcctacat tcccaggtgt tggtattgga gatattgcca
    28621 tatataaaaa tgcttgtaat gtcagtatat aaatatagct ataaccttgt aaattaaatg
    28681 ctttatatat ccaaaatgaa acactaaagt aagcacttaa ttgaataaaa gttactatta
    28741 tagttgaaat taataccttt ttattctttg caaaaaaatt tattgcttta ctataatcat
    28801 ctataaaact ttctatggat tcaatcttat tatctaaaaa ttttaaaaac ttaatttttg
    28861 ataaatgctt tatagtaatt ctcataaaat attttatttt tttaggattt aaaataacta
    28921 atattatcat aattaagaca aatgaattaa tggctatccc tagaaacact agaggcataa
    28981 tcattttcat ttgtttttct aacatagcaa aattcattaa aacaagtatt gtacaataaa
    29041 aagtaactac tatttggaag ataatagact tattggtaac aactgcactt gccttactaa
    29101 gtggcatttt acatttttta agtacatata tctgtactgg ttggcttcct gaagcaaaag
    29161 gagttattaa attataataa aatcccataa tggctagctt aaaaccaata aatctaatat
    29221 taactttgtg agtgctttct ataattatct tcattactac tccttcaagc attatgtgac
    29281 acattattgc caaagcacct atgaataaaa acttcttatc taccataacc ataacatttg
    29341 atagcatttt aatatccaat gttttaaaca ctaggtaaat tgttatacct attaacaaaa
    29401 ataaaaatgt atactgtagt aaactctttc taatattctt tagtctatcc attagaaata
    29461 accatccttt gctcaagatt tatactaaat actatattat tagtatacac tatgaatctt
    29521 aatattttat aacaaaatac cttacaattt tgtcatttac aaaaaatatt aatctataaa
    29581 ttaaatttta atatatagat taatatttta caaacatttt gtagatatat ataatatttt
    29641 cactaaacaa cacttatact tattacaccc atttataccc tatcccccat acagtttcta
    29701 ttgtataaat atcatattgt tttaatttgc ttcgtatatt ttttatatgc tctgtgatag
    29761 tacttatatc actttctcca tcaaaaccat ataccgactc atatattctt tcttttgaaa
    29821 atacttgtcc cttatttttt gctagaaatt cacatattag atattcactt ttagtaaaat
    29881 taattttttt accatcaatt gaaacttctt tgcctaaaaa gttaaattga acatttgaga
    29941 tagttaataa attctttttt tctctacttt ctcgccttaa atgagcagtt atcctagccc
    30001 ttaattctcc tacaccaaaa ggtttggtta tatagtcatc tgcacctata ccaagaccgt
    30061 acataatatc actttccata ttttttgctg tcaaaaacag tattggacaa tcaaccttat
    30121 ttcttatagt tttacatact tcaaaaccat ccatcttagg catcataaca tctaatatta
    30181 ttagattaaa ataattaaaa tccatgttca tagctttttc tacatcacta attaccttta
    30241 cttcatgtcc atctcttttc aacacatttt caatcaactt taacatatct aactcatcat
    30301 ctactactaa tatcttagac ataataactc ctccatttat ccgtttaata gtattctaat
    30361 ttatatattt aataatatct ttaaaattca cattaaccat aatactctca tattatagct
    30421 tacaataatt aattactatt ttacaaataa aatattttag atacaaaaac tttatatttc
    30481 aaaaacttta tattca
//
LOCUS       DBGJFU010000037        30374 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.037, whole genome shotgun sequence.
ACCESSION   DBGJFU010000037 DBGJFU010000000
VERSION     DBGJFU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.037"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..1053)
                     /locus_tag="ACOIAS_001630"
     CDS             complement(<1..1053)
                     /locus_tag="ACOIAS_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HGZ2701344.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNK"
     gene            1120..1491
                     /locus_tag="ACOIAS_001631"
     CDS             1120..1491
                     /locus_tag="ACOIAS_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HGZ2701345.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1528..2202
                     /locus_tag="ACOIAS_001632"
     CDS             1528..2202
                     /locus_tag="ACOIAS_001632"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGZ2701346.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2451..3290
                     /locus_tag="ACOIAS_001633"
     CDS             2451..3290
                     /locus_tag="ACOIAS_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGZ2701347.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3534..3630
                     /locus_tag="ACOIAS_001634"
     tRNA            3534..3630
                     /locus_tag="ACOIAS_001634"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3568..3570,aa:Sec,seq:tca)
     gene            3785..4492
                     /locus_tag="ACOIAS_001635"
     CDS             3785..4492
                     /locus_tag="ACOIAS_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HGZ2701348.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4634..5470)
                     /locus_tag="ACOIAS_001636"
     CDS             complement(4634..5470)
                     /locus_tag="ACOIAS_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2701349.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5658..6563
                     /locus_tag="ACOIAS_001637"
     CDS             5658..6563
                     /locus_tag="ACOIAS_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HGZ2701350.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6572..7756
                     /locus_tag="ACOIAS_001638"
     CDS             6572..7756
                     /locus_tag="ACOIAS_001638"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2701351.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            7871..9172
                     /locus_tag="ACOIAS_001639"
     CDS             7871..9172
                     /locus_tag="ACOIAS_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HGZ2701352.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            9126..10169
                     /gene="holA"
                     /locus_tag="ACOIAS_001640"
     CDS             9126..10169
                     /gene="holA"
                     /locus_tag="ACOIAS_001640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGZ2701353.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(10356..10622)
                     /gene="rpsT"
                     /locus_tag="ACOIAS_001641"
     CDS             complement(10356..10622)
                     /gene="rpsT"
                     /locus_tag="ACOIAS_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGZ2701354.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            11218..11739
                     /locus_tag="ACOIAS_001642"
     CDS             11218..11739
                     /locus_tag="ACOIAS_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2701355.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            11773..12549
                     /locus_tag="ACOIAS_001643"
     CDS             11773..12549
                     /locus_tag="ACOIAS_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HGZ2701356.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            12891..13844
                     /gene="gpr"
                     /locus_tag="ACOIAS_001644"
     CDS             12891..13844
                     /gene="gpr"
                     /locus_tag="ACOIAS_001644"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HGZ2701357.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            13862..14881
                     /gene="spoIIP"
                     /locus_tag="ACOIAS_001645"
     CDS             13862..14881
                     /gene="spoIIP"
                     /locus_tag="ACOIAS_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HGZ2701358.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            14912..15163
                     /locus_tag="ACOIAS_001646"
     CDS             14912..15163
                     /locus_tag="ACOIAS_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701359.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            15445..17250
                     /gene="lepA"
                     /locus_tag="ACOIAS_001647"
     CDS             15445..17250
                     /gene="lepA"
                     /locus_tag="ACOIAS_001647"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGZ2701360.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            17399..18736
                     /locus_tag="ACOIAS_001648"
     CDS             17399..18736
                     /locus_tag="ACOIAS_001648"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HGZ2701361.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            18969..20282
                     /locus_tag="ACOIAS_001649"
     CDS             18969..20282
                     /locus_tag="ACOIAS_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2701362.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            20454..21629
                     /gene="hemW"
                     /locus_tag="ACOIAS_001650"
     CDS             20454..21629
                     /gene="hemW"
                     /locus_tag="ACOIAS_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGZ2701363.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            21732..22751
                     /gene="hrcA"
                     /locus_tag="ACOIAS_001651"
     CDS             21732..22751
                     /gene="hrcA"
                     /locus_tag="ACOIAS_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HGZ2701364.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            22781..23401
                     /gene="grpE"
                     /locus_tag="ACOIAS_001652"
     CDS             22781..23401
                     /gene="grpE"
                     /locus_tag="ACOIAS_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGZ2701365.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            23493..25340
                     /gene="dnaK"
                     /locus_tag="ACOIAS_001653"
     CDS             23493..25340
                     /gene="dnaK"
                     /locus_tag="ACOIAS_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGZ2701366.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSESEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            25551..26705
                     /gene="dnaJ"
                     /locus_tag="ACOIAS_001654"
     CDS             25551..26705
                     /gene="dnaJ"
                     /locus_tag="ACOIAS_001654"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGZ2701367.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            27671..28606
                     /locus_tag="ACOIAS_001655"
     CDS             27671..28606
                     /locus_tag="ACOIAS_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2701368.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKKRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            28642..30015
                     /locus_tag="ACOIAS_001656"
     CDS             28642..30015
                     /locus_tag="ACOIAS_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2701369.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(30203..30361)
                     /locus_tag="ACOIAS_001657"
     CDS             complement(30203..30361)
                     /locus_tag="ACOIAS_001657"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2701370.1"
                     /translation="MSKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYLSVK
                     SYFLYKKI"
BASE COUNT        11997 a         3265 c         5214 g         9898 t
ORIGIN      
        1 tttatttaat ttttcaatag aaaaagattt atctgtaagt ttaaaattat cttttatacc
       61 cttagttaag tatactttct tatccttagt tatgaatata gcatctacat tatcaatttt
      121 attaattaaa ttcatacctt tttcaagacc aagaccaaat gtagaagtgg ataaggcatc
      181 acaattaata gatttatcag agattattgt aacactactt aaattgtttt caaaaggata
      241 ccctgtaaat ggatttaaca tatgatgata tatttttccg tctttttcga taaatctttc
      301 ataaattcca gaagtaacaa cagacttatt tgtagtttct atgtttccta tggattcacc
      361 atttttacta gttgggtctt gaatacctat tttaaaaggt ttatcatttt taccattttc
      421 taaagtaaat atatttcctc ctaagtttac taaacctttt tttacatcct ctgattttaa
      481 ataggctaca attttatcag cagcatatcc tttagctatg gcacctaaat caattttcat
      541 attttctttg gttaatttta tagatttatt tttttcatcc aatatgatgt tcttataatc
      601 aataagaggc agaagttttt gtatcttaga atcacttgga actttagctt tatctgttcc
      661 aatagcccat aaatttgata atggaccaat tgttatgtca aaatatccat ctgataattt
      721 ggaatattgt atagcttctt ttacgacgaa aaatgtatca tcagaaactc tgacaaattt
      781 tttaccagca ttatcattta ttttagaaac atcacttcct ggtatatgag tgctcatttt
      841 gttgtcaata tatcttaaaa ttgaatcaca ttcatttaag attttatcac ttttggattc
      901 ttttacatta aatacagtaa cttcgtttac tgtaccaagg tagtagctag ttttactata
      961 tttagtattc tggcttgaac tcctaaaaaa tgaaaataaa attaatgcta aaatcaatat
     1021 cacaaaaata aatgtgattt ttttcttatt catggtatat tcctcctatg aaattgtgtt
     1081 attattttat tataacataa tttcatagga ggacaaaaaa tgaagaaaaa agacattctt
     1141 cttattttag gcattttggt tgttattaca gcatgttatg ggataataaa tgttataaat
     1201 tctaaaaatg ctggaaatat tgagatttat gtagataata aattgtataa aactgtttca
     1261 ataaatgcca aagaagaatt taaaatagag agtagagggg ggtataacat agtaaagatt
     1321 catgataaag gggttgagat agtagatgct tcatgccctg ataaggtttg cgtacatact
     1381 ggatttataa acaagccaag tcaaagcata gtttgtatac ctaatagagt gagcataaaa
     1441 ataaatacaa atgaaaagaa tgataatcaa gaagatataa tttctaaata aaataataaa
     1501 aataattgaa aggaattggt gataattatg gttaacttag gaaacgattg ggatgaatta
     1561 ttgaaagaag aatttgaaaa agattactac ttaaacttaa gaaaatttct tatagaagaa
     1621 tataaaacac gccaaatttt cccaaatatg cacaatatat atgaagcttt aaaacacact
     1681 tcatacaaag acacaaaagt cttaatatta ggacaagacc cttatcatgg agataatcaa
     1741 gctcatggtt tagctttttc agttcaacca caagtgaaaa ctccaccatc tttattaaat
     1801 atgtataaag aattaaaaga tgatttaggt tgttttatac ctaataatgg atatttaatg
     1861 ccttgggcag accaaggagt gttactttta aatactgctc ttactgttag agcgcatgaa
     1921 gctaattctc ataagaacaa aggatgggaa attttcactg atagagtaat atctatttta
     1981 agtgagcgtg aagacccagt tatttttgta ctatggggaa gtaatgcacg taaaaaagta
     2041 gagttgattg atacaagtaa acattatatt ttggaagcac ctcatccaag cccattgtca
     2101 gcaagtaaag gcttttttgg atgtaaacac ttctctaaga taaatgaaat tttgaaaaag
     2161 ctaggaaaag aacctattaa ttggcagata gaaaatatat aaaagtaaaa ggtaacaaaa
     2221 tgataacaaa acaacaataa acaatgacat tgttgtaact aatagaaata tgcttaaata
     2281 gttgaaaata ctatgtatgc atatttctat ttttttgtca agttgacaac tcttgaaaaa
     2341 atagttacaa tttagttaca caaaaaacac aaataaaaaa agaaacttac aaagcagaat
     2401 tttatgaagc aaaatatata gtgtaaaaaa tcttagggag gaaattttat atgaatttta
     2461 aaaaattgag taatgctaaa gtctcggtaa tggcagttgt tttatcagtt ggaatattat
     2521 cgggatactc tgcgcttaat aaacaagtaa cattagtagt aaggggagaa gaaagaacta
     2581 tatcaacatt tagttctaat gtaggagacc ttttagaatc tcaaaacatt agctatgatt
     2641 caaatgatat agtaacttta gatgaaaata caaagttatc taatggagat aaaatagaag
     2701 taattgatgt aaaggaaaaa acagttgtag aaactaaaga cattccattt gaagttacag
     2761 ttgttgaaga tggctcttta ttaaagggtg attctaaggt taaagaagaa ggaaagtctg
     2821 gtaaaagtga attaatatat aaagaaactt atcacaatgg aaagaaagta gaaaaggaat
     2881 ttgtgaaaaa ggttgttaaa gctgagccag taaataaggt tgtaagcaaa gggacaaagg
     2941 tagaaatgca agtagcctca tcaaggggag aaagttcaag aagaatggct tcatcatcat
     3001 ctagtagcag tagtagtaac aatggtaaaa atatgagagt ggtagcaact gcatatactg
     3061 gtcacagtat aactgcaaca ggaacaaaac ctaagtgggg aacaatagct gtagacccta
     3121 aggttatacc atatggaact aaagtttata tacctcaatt tgacatggtg tttactgcag
     3181 aagactgtgg tggagcaata aaaggaaata aaattgatat ctacatgaat gattcaaaaa
     3241 cagtttataa ctggggaaga aaaactatag atatacgtat attaaaatag ttaaataatt
     3301 tatattttta ctggaaatta tattttgatt atatagagaa gaaactcttt atagattcta
     3361 aaaatataat ttccagtttt ttattttgga tatgtaataa aacaggtaaa ttttaaccat
     3421 gtatagataa aagctattta aattataatt tactatcata taaatctata aaaattgttg
     3481 caaagagtca aaatatatga tattatagat ttcgttgaga aggatttcat tatgggagtg
     3541 gatgggtgcc ggtgtgccct ctagtcttca aaactagtat gaggggttaa gagcctctta
     3601 ggtgggttcg attcccacgc attcccgcca atatgtaaaa ggtatctttt aattaaaaga
     3661 taccttttta aatttatttt aagataattt atttttaata atatataatg ttttgtatta
     3721 tataatgtaa attttctaat acaaaacatt atatatttag ataaaaatct aggagtgtgg
     3781 aaagatggaa aataatttta caacgagaac tagttttcta gttggagatg atgggataaa
     3841 aaaacttaat aattcaaata taatagtatt tggagttgga ggagttggta gttttactgt
     3901 tgaagcttta gcaagagctg gagtaggcaa tattactatt gtagattttg atgatgtaga
     3961 tataaccaat ataaatagac aaataccagc cctacattca actgtaggaa gatacaaagt
     4021 tgatgtcatg gaagaaagaa tattagatat aaatcctaat atcaatataa aaaaaataag
     4081 aagtctttat aataaagata ctagtgacga aatactaact gaaagatatg attatgttgt
     4141 ggatgcgata gacatggtaa gttccaaaat acatctaatt gaaacttgtg aaaaaaaagg
     4201 attgaaaata ataagttcta tgggtatggg aaataaatta gacccaacta aaatagtcgt
     4261 tacagatata cacaaaacaa gtacttgtcc tctagctaaa gttatgagga aagaacttag
     4321 ggatagaggt atcaaaaagt taaaagtagt atattctact gaacaaccaa tagaacttaa
     4381 gaagaaagta atgaatggta gaaaagtaac acctggtagt gtatcatttg tcccatctgt
     4441 aggaggttta ataatcgcat ctgtaatagt aaatgaacta ttaggacaat aagttttaaa
     4501 tattttaatt tataaatttt atatacaaat ttgaaggcta gaagcatgaa tacaaagcaa
     4561 ttttatattc atacttttag cctaattatt ttatattacc ttattttatg tagttgtagt
     4621 tttatttttt agattatgta tctatttcat aattatattt tgtaaatata atttctccat
     4681 cactataaaa aacaatatct cctattctat ctgttctata aattgataca tggttttgtt
     4741 ttagattatt aataacctcc ctatgtggat gaccatattg atttttatat ccacatgata
     4801 ttgcagctat atctggacta atttttttta taaattctaa agatgaagaa gaattacttc
     4861 catgatgacc tatttttata aaattaatat tatttaattt aaaagaatgt aaaatttctt
     4921 tttcattttc ttcttcagca tcacccataa ataaaaaaga attatcatta aaagttaatt
     4981 tgaaaactat agaatttaaa ttagattctt cttgtatata tgatggactt aatacatata
     5041 tatttatatt gttatctatg tttaaaacat catttttata gagatgctga atagataagt
     5101 ttttatccgt acaagaattt attagatttt gataagcctt actatcagtt gattgttcag
     5161 gcatatatat ggagtttaca ttaaattttt ttataatatt atcaagactt cctatgtggt
     5221 ctgaatctgg atgagttgct attataatat caatagtttt tattcttttt tgtctaagat
     5281 aactaataat tatattttcg ctatcttcat cacctccatc aattaatata ttcttatttg
     5341 tgggtgtttg aactaaaata ctatcacctt gtccaacatc aatcatatgt atagatagta
     5401 gagatttttt gtcacagccg attaaaagtg atattataat aagtattgat aaaaaaattt
     5461 tatttctcaa taaaaaatca ctccattcat tacatgttta tataaattta tgatgggtaa
     5521 tataagatat atatgactta ttatatttta tattttaaac aatataaatg aaattaatac
     5581 cataataaaa ttggagtttt ttatatatac aaaataaaat ttttaaaatt aagcaataaa
     5641 aaaggtagga ggattttatg tttttcaaag atattgaact aaattcaaaa aaagagttag
     5701 acccatattt tgatttagta gattatgaag cttgtgagta ctgttttagt actttatata
     5761 tgtggcaaca tgtttataag acaggatatt atataggtga agattttgcc gttttagttg
     5821 gggaatacga aggagatagc ttctcaatat taccacttgc taaaaaagat aaacttccag
     5881 aagttgtaga ttttgtcctt gaatattttt ctaaaaataa taaaaaaata tacttaagag
     5941 gtattactac tgaagtagta gagtttttaa aagaaaaata tccaggtaga tttgagtata
     6001 tagaagaaag agatttattt gactatattt atgatgcaga aagtttaaga actctagctg
     6061 gtaaaaagaa tcaaaagaag agaaaccata taaactattt cttaaaagag tatgcaggta
     6121 gatatgaagc taaattatta gataaagaaa actttgatga gtgtttagta ctaatgaagg
     6181 aatgggaaag taataaggaa gaaaataatg aatttgatga aagtatggat gatgaattaa
     6241 taggtatcaa aaagatattt aaccattatg acatattaaa agataaggta aaagtgtttg
     6301 gtgtttatgt agatggaaaa ttagaagctt ttagtatagg agagctttta aatccaaata
     6361 tggcattgat tcatatagaa aaagctaatc ccgatataag aggtctgtat ccatttataa
     6421 atcaacaatt tttggtaagt gaatttaagg acgttgagtt tgtaaataga gaagaagatt
     6481 taggtattga aggattaaga aaagctaaac tttcttatca tccttgcaga tttgttgaaa
     6541 agtacagtgt tagggaggct taatagacta tatggagatt aggtatgcta aagaagaaga
     6601 gatagaaagt ataaaagata tttggagtta ttgttttaac gatacggaga gttttatgaa
     6661 gtactatttt aatgataaat ataaatctga aaatactgta gtagctttag atgaaggaaa
     6721 aattatttct tctcttcagc taaatcagta taagttatta ctaaatagca aggtatataa
     6781 tacatcatat gtggtaggag tttctacact tccagaggga agaggagctg gatatatgaa
     6841 caaagtaatg aaatttactt taaacgaatt gtataaaaaa gggcaattag tttctatatt
     6901 gatgcctatt gactatagac tttatagaag atttggatat gagcattgtt acgaccagat
     6961 tgaatatact attaacacag atgatttaaa aaattttaag tcaagtggaa aaatgataaa
     7021 gtctaattta tcacaaattg atgatttgat acaaatagat agaacttttt taaatgaggt
     7081 taatggtaat gtgttaaaag atgaacatta ttatgaaaac ttatttaaag agattcaaag
     7141 tgaagatgga tttttataca ttcatgaagg aaatgaaaaa gatggatata tagtttattt
     7201 tcttcaggaa gataaaatgt ttgtcagaga gttattttat aaaaatatag atgcccttaa
     7261 atcaatgctt aaatttatat ataatcacaa tactcaatgt aagatagtta ctatatctac
     7321 tcctacaata gataagataa ggtttatatt agacaaccct aaagatagtg atataaaaat
     7381 aaaacctttt atgatgggaa gagttataaa tgtaaagaag tttattgaag atatagatat
     7441 tgagaaggat attaatagct catttaattt attaatagaa gacaagttta tagatgaaaa
     7501 taatggtcta tttaaaattt ctatacagaa taagaaggta tctgtagagc agttagataa
     7561 aaaaggtgca gaaaagcctc aagaagattt tgatattaaa ttagatataa ataccttaac
     7621 acagttatca ttttcttata tagatgtaaa tgaagctatt ttcttaaatg atataaaaga
     7681 tgtttcagaa gaaacactag aaacacttaa ttgtattttt tctaaaaaaa ataactatat
     7741 aaatgaatat atataggtag agtacgttaa aagattctaa aaatcttttg acgtattttt
     7801 ttgtttagat aacaatttat cttgtttaaa aattttctat ttgaaaataa tctaaataat
     7861 atacttttat atgggagtga ttataattca aataagaaga ccattattaa ttatattaat
     7921 gtttgtggta gctatatcat ttatcattac aaataacaca aaaaacgacg aagaacttaa
     7981 tgataagata ataactatac aaggagttgt caaaggaaaa attgaaaaga aaagatacaa
     8041 tcagtacaag gtaggggttt ttctaataaa tgattataca aaaaataaaa atttgaagat
     8101 tggtcagaaa gttaatatta caggtaaatt caaaagttta gataaaatga agtatgatga
     8161 ttttgattat ggaaggtata taaaaagtac tggatataaa ggtatagtgt atataaataa
     8221 ctataaaatt attgataaaa ataaacttta ctctttaata ggggaaataa agttttatat
     8281 tagtaaaact tataggtact tatataagaa aaactcagat tttataaatt ctatactatt
     8341 agcagaagtt gaaaatttga ctgaggaaca aaaagaaatt tttaccagaa ctggaactag
     8401 tcatgtaata tctatatcag gtcttcatac aggaatatta tgtgtaatta tatcctttct
     8461 attaagaggt ataaataaat tgtataaatt attaatactg tcgattttta taactttata
     8521 ttgtataatg gtaggtgcgt ctccatccat aattaggtct atagcatttg ttatgatttt
     8581 ttatttatca atatttattg atagaaagaa agatggaata tcggcattgt ctctaattgg
     8641 gataatttta attatgaata acccctatgt tatatataat gtaagttttc aattgtcttt
     8701 tttagctaca ctttctatat taatattcta taataaaata aatagtatta taaaattaag
     8761 tatggtatca ttgacaatat catcaaatat attaacttta cctataattt attatacatt
     8821 taaaggtata ccattgcttt caattatagg aaatttaatt atagtgccat ttgtgggagt
     8881 aataatgtat ttaagtattg caagtttaat cgtatttaaa gtaagtgtag taatagctaa
     8941 aattatatct ttttttaata gtacatttat agaaagcata ttttttttac tagaaaaaat
     9001 cagcaattta agttttgcat acattaatat agaaaatcct aaattttata ttgttgttat
     9061 ttattacata ggagtgtttt tttacatatt ttacatagaa ggaaaagaga taaaggagca
     9121 agagaatgaa ttacaaggat attataagga atgtaaaaga gaaaaacttt gaaaaaatgt
     9181 atctttttta tggaagagag tattatttaa ttgagaatgc aataaaagca tttaaagata
     9241 gtctaaacga gggaatgctt gattttaatt tagatataat agatgggaag gaaatagtct
     9301 taaatcagct tataagctct atagaaacac ttccatttat ggatgatagg aagatagtaa
     9361 taataaaaga ctttgaacta ttaaaaggaa agaaaaagaa ttttacagat agtgatgaga
     9421 aatacttaat agagcattta gacaatattc cagacaccac aactatagtt tttgttgtat
     9481 atggagatgt agacaaaaga aaatcattag ttaagaaaat aggaaacaat ggaattgttt
     9541 ttgattgtga taagctttct gatatggatt tgtttaaatg gataaaaaaa tcttttgctt
     9601 taaatgatgt tataatagat aattctcaaa tcatgtattt tattgaacaa gaaggataca
     9661 gagataagag tagtgaaaag acattatctg atttagaaaa tgaaataaat aaaataagtt
     9721 catttgttgg taaagggaat aatgtaacca atgatgtaat tgataaatta tctcaaaaaa
     9781 aagttgagaa tgatatattc aaattaatcg attatattgg agaacaaaat gcttctaatg
     9841 caatgaaaat attaaatgat atgatacaag aaggcgaatc tgtattaggt atattttcta
     9901 tgatagcaag acaatttaaa ataattatgc aagtaagaca gttacaactt gatggatatt
     9961 caacaaaact tatagctgat aaattaaaaa tgcatcaatt tgttgtagga aaagcattaa
    10021 agcaaactaa aaatttttct gatgatataa ttgtggaaat attaaactat atattagaaa
    10081 gtgattacaa aataaagaca ggtcttataa gagatacttt agcagttgaa atgttggtta
    10141 gtagatactg taaaagagaa gcaatttaaa aaatctatat attaataaaa aataaatagc
    10201 aagatatgtt atagttcaga gtagatgtaa gctaaattaa gtttgatata caatcaacta
    10261 taatatgtct aactatttat tttttattgt ataaatttac aaaataaaaa acccctacat
    10321 aggggttatt atatataaga aatataaact ggtaattaag cgttcatacc gtttaatttt
    10381 tgagctaatt tagctacttt tctagctgca gcattcttat gtatagttcc tttagaagct
    10441 acttggtata ttcttttttg agcgtattgg aacttagcaa cagcatcctc tctgtttcca
    10501 gctgcaacag catcttcgaa tcttcttatt gcagttttta tttgagattt tctaactcta
    10561 tttaaagcag ttttcttttc gataacactt attcttttct tagctgattt tatatttgcc
    10621 accgtgttca cctccctgta tttttttatc atattcatcg tcaacatatt agattttaac
    10681 agaaataaat ctaaaaatca aggattatta tttatatatt ttaataatgt tttttatttt
    10741 gattttttag actgtaaatt gtgtataaat aatttgcaat atcttgtttt taaattggta
    10801 tattttgtgt tttaaatatt ttgacatata taattaatat ttcattttgt aacaaatatt
    10861 tgagataata caaactatta acatacattt tgatagtatt tccaataatt ataagataat
    10921 acaagaaatt actcattttt gtaaatttaa tgaaaaatta taaaaatata aatttatatg
    10981 cttaattaag ataatttttc caataatcaa gataattttt ttcattagta tgtatgatag
    11041 atgcatcaaa caatataact aatatgttag aaggttatat gtaattaatg tcagtgttta
    11101 tatagaatag catttgtagc atttattata gttgtaatta ctatttatat catagacttg
    11161 aatagtctaa aaaaatagac tataatattt ttatagacta aagaaagggc tgataagatg
    11221 gacatagcaa aactaatttt aaatcctgca aggcttcgta ttttacaata tattcgactt
    11281 tatggcagtg ttcgtacatc tgatattgta aagtatttaa atgatattcc tcgtgcaaca
    11341 gtttatcacc atgtaaaaat tttagaagag aacaatatga ttgaagtaat taaagaaaat
    11401 cgtgtgcgtg gtactattga aaaggtttat gcactaaagg aatatacaac atctatggaa
    11461 ggagagacct tcgttgcact ttctacagca ttttatgtag gtctgatgca ggaaatgaat
    11521 gaatacttta gcagaaaaga gcaagaccat aaaaaggata atgtattttt ctcttcagca
    11581 ttactatata tgagtgataa tgaatatgaa aatcttctta aaagtatagc tgatttattg
    11641 aaaccatata tagaacaaaa acctaaatca aatctaaaac ttagaaaact ttcaattata
    11701 tcttctccac ctgtagctga taatgataaa acaaaataac atgcaaccca tttttataaa
    11761 aggaggttat atatgaaatt tcatgagttt ggaaataaaa acagaccaca tattatgttg
    11821 attcatgggg gagggaatgc atggtggaac tatttgcgac aagcagaagt tttgtctaac
    11881 aaatatcatg tgatacttcc tacattggat ggtcatggag aagaatatga aacaacatac
    11941 atttctacag aggatacagc agataagttg atatcatata tagatgaaaa ttgcagtgga
    12001 catcttttcg ctttgtgtgg ggtatcactt ggtggtcaaa ttgtcatgga acttttatct
    12061 cgaaaaccta atattactaa aaaagcaatt attgatggta gtatttgcta cccaagacct
    12121 cttatggcaa ggttttgtat tgctggtatt cgattttttg gaggactttt gtttagtgag
    12181 aaggcttgtc gctttcaaat tgctatgatg ccaaaattgt tacctgaaaa tatgcaatat
    12241 ccagatgaaa taaaagcgta ttatatgaca gatatgccac atgtaagaaa agaaacattc
    12301 tataatatgt atcgtactta catgatgaac tatacactta aagagaatgt taaaaaaacc
    12361 acagcacaag taatgtactg gtatggagaa aaagaaatga agtgtgtcaa aaaatctgca
    12421 aaactgtttc agtcttatgt gccctcttgt gagatttatg aagctaagag ctataatcat
    12481 gggtatttat cactttatct tccaaatgaa tggttggaaa ttgcagaacc atttttccaa
    12541 aaggaataaa ggtgttaggt gattattgta taattattgc tgttttttta tatagtaaat
    12601 aaattaggta taaatataaa aaattatttt agttaatata ttagtgaaag cttaatagga
    12661 aaattataaa tagattaaag gtagcaccta tataaatagt tgctaccttt ttctgtttgt
    12721 aaataaatga tagataagaa ggtttactga caaatcactg actaaaccca attttcttac
    12781 attgaaagcc ttaattttac ttgttttaaa taaatttaca atttcttgtt ataaaatact
    12841 aaattttgtt aacaattata attagcattt aatttatact ggaggtttat atgattagtg
    12901 taagaacaga tttagcttta gaagctagtg aaatgtgtga aaagtctcaa gaaggaagta
    12961 gtattcctgg tgtaaaaata gaaactaaag aattagaaaa ttgcattgta actaaagttg
    13021 aagtaataga caggcaaggc gcagagataa tgaataaaga tataggaaag tatataactc
    13081 tggaaagtaa tcttatgaag tttgatgatg atgagtcaag agaagaaatg ataagttatt
    13141 taaaagagga gttagtagat atatttggcc aagacaaaaa taaaaaaacc ctggtaattg
    13201 gtcttggaaa tagaaatatt acttctgatg cgttaggacc aaagagtgta tcaaaaacac
    13261 ttgtaactag acatcttttt aaaaattata ataaagacta tgatgatgat ttcactgagg
    13321 tatcagcttt aagcccagga gttatgggtg ttactggaat agaaactagt gaaatagtaa
    13381 aatcattggt tgagaaggtt aagccagata gagtagtagc aatagatgca ttggcttcaa
    13441 gaaaaatgga gagagtaaac tctactatac agatatcaac agcaggaata tcacctggtg
    13501 gaggtgttgg caatactaga aaatctctaa ctaaagaaac tttaggcgtg gatgttattg
    13561 caataggcgt tccaacggtg gttgatgcag caactcttac aatagatgtc ttagatatgg
    13621 caatagacaa tttaatagct caatcagaag aaacagaaag tttttatgaa atgttgaaaa
    13681 aacttaaaga agaagaaaaa tatcatctaa taaaagattc gttagaccca tatgacaaga
    13741 acttaatagt aacaccaaag gatatagatg atacaatcga aaatctatct ataataataa
    13801 gtgaaggctt aaataggtct ttacatccag gtagattggt ataaaataag gaggagcaat
    13861 tatgtttaaa aaatgcatta aagtagtaac cttgactttt atattagtat gcattttacc
    13921 aggaaaatca ttagctttga atcaagatga ttttttaaag tttttagtaa attcatctta
    13981 tccagaagct aaagtcgaag gaaatgatac agaaaataaa aagaataata aaaataaaga
    14041 aacaagtaag gaaaataagg aagaaagtaa agaaggaaac actaaatcta aagatgcaag
    14101 taaagttgat aataaaaaag agagtgaaaa agaatatata aaactttatg ttggtaaaga
    14161 aaatgttcca gatattgagt caaaaaattc agacacaact gaaactaaca caacatctag
    14221 ttcagattat aaggatgatt taagagtaac caaagaaaat ccaagaattt tgatatatca
    14281 tactcatgga tgtgagactt attcaaattc acctgatgga aattatcatt caagagataa
    14341 gaaaaattca gtaatggaag taggaagtgc attaactagt gctttagata gcaaaggatg
    14401 gggtgtagtc catactacta aataccatga ctatccatct tataataact cttatgcaag
    14461 tagcttaaaa acaatacaaa gtatattacc taaatataac tcagtggata tagcgataga
    14521 cttacataga gatgctaggg atttaactaa tccagccact aaggaaaaag accatttaaa
    14581 atatacaact atgataaatg gagagagagt atcaaagttc ttctttgttg taggaggtaa
    14641 aaatacaaat agaaaacaac ttagagcttt agcagaagat ataactgctt ttgctgagaa
    14701 aaagtatcct ggtctagtat ctccaatagt agaaaaagac tatgctagat tcaatcagtt
    14761 tgcagttaag aatcatatgt tagtagaaat agggaataac gcaacaagtg tagaagaatc
    14821 taaggctact acaaagtatt tagccgagat tttggatgaa tattttaaac aaaaaaatta
    14881 gagctttaag ttataattag gagcatgcta aatgagccga ttagaaaaaa atatagaaag
    14941 taaaagtaaa ctaagaaaat acaagtatat tatgagaatc ttatttttga ttactatgat
    15001 tatatgtaca tcagtttgtg tatttatagt ggacagtaat gcaaaaagta tgctaggaat
    15061 gaaacaatat gattttccaa gtattaaatt tgatataaca aaatttgatt ttgacatacc
    15121 aaattttgat atacatgaaa tcataaaaaa atttaataag tagtaataag tgaataaggt
    15181 ttattgatta aaataagcct tattttagtt atatattttt aatgtataaa ataaaaattc
    15241 attaactgta ttttatagga tttactttaa gtttatgata ttatataata tttagtgtta
    15301 gacttgataa tccacatata atgtggataa ttttgtttag tgccaattag tttatattca
    15361 aaaattttaa atttggtata taaagagttt ttagataatg taaaataata actataagaa
    15421 taatcaagag gagggaattt taaggtggac aataaacaaa gtagaactag aaattttagt
    15481 ataattgcac atatagacca tggaaagtct accttagctg atagattaat acaacaaact
    15541 gggcttgtta gtgaaagaga catgaaaagt caattacttg acaatatgga ccttgaaaga
    15601 gaaagaggaa tcactataaa gcttcaaaac ataagattaa tgtataaagc gaaggatgga
    15661 aatgaatatt atttaaatct tatagataca cctggacatg tagactttaa ctatgaggta
    15721 tcaagaagtt tagctgcatg tgaaggagca ctattagtag ttgatgcagc tcaaggtgtt
    15781 gaggcacaaa ctttagccaa tgtttatctt gctatagacc aagatttaga aatattacca
    15841 ataataaata aaatagattt accaagtgca agaccagagg aagtaaaaaa tgaaatagaa
    15901 gatttaatag ggcttgattc aagtgaagct cctcttattt cagctaaaac tggtttaaac
    15961 atagaagatg ttttagaaga tattgtaaaa aatgttcctc cacctaaagg agataatgaa
    16021 gctccattaa aggcattaat attcgattct tactatgatg cttacaaagg tgttgtagct
    16081 tatgttagag tatttgaagg aactgttaaa aagggtatga caataaaaat gatgaatacc
    16141 aataaaaagt ttgaggtaac tgaagttggt gttatggctc caggtcaaac tgaacttagt
    16201 gaattatcag caggtgatgt tggttatatt gctgctagta taaaagatat aagaagttgt
    16261 cgtgttggtg atacaataac agattcaaat aatcctacag aagaaccttt accaggatac
    16321 aaaaaagcta ctccaatggt atattgtggt atatatccag gagaaggaga aaagtatgag
    16381 aatgttagag atgctcttga aaagcttcaa gtaaatgatg ctgcacttga atatgaagct
    16441 gagacttcag cagcactagg atttggtttt agatgtggtt tcttaggtct tttacatatg
    16501 gagataatgc aagaaagact tgagagagaa tttaaccttg atataattac tacagctcca
    16561 tctgttatat atagagttac aaaaatggat ggagaggttg taatgataca aaatcctgca
    16621 aacttaccag aaccatctga gataaagatg atagaagagc ctatagtaaa aggagatata
    16681 atagttccta aagactatgt gggtgttgtt atggagcttt gccaggaaag acgtggaaat
    16741 atgttaaata tggaatatat agatgataga agagtaatgc ttcactatga ccttcctctc
    16801 aatgaagttg tttacgattt ctttgatgct ttaaaatcaa gaactagagg ttatggttct
    16861 cttgattatg aggttaaagg ttatgtagca tctacacttg taaaacttga tatattaatc
    16921 aataaagagc aagtggatgc acttagtttt attgtccatg agacacgtgc tttccctaga
    16981 ggaaaggcaa tgtgtgagaa attaaaagga gaaattccaa gacatcagtt tgctatacca
    17041 atacaagctg ctgttggtaa taaggttatt gcaagagaaa caataagcgc tctaagaaaa
    17101 gatgttcttg ctaaatgtta tggtggagat atatctcgta agaagaaact tcttgaaaaa
    17161 caaaaagaag gtaagaagcg tatgagacaa attggttctg ttgaagttcc tcaaaaagca
    17221 tttatgtctg tgcttaaatt agatgagtaa atccttataa atttagataa agatatttaa
    17281 aagctagagt gattttagaa ttatttctaa attagtctag cttttttatg gtatatttta
    17341 agtaaactca tagagaatat aagtaagaat attaaagaag aataaaaagg tgatatttat
    17401 gattaatata gaaattccta aaaaagtaga ctatataatt aaagaattag aaaagaatgg
    17461 ttatgaagct tatatagttg gaggatgtgt tagagattgt ttattagaaa gagttccaaa
    17521 cgattgggat ataacaacta gtgctagacc agaagtggta gtagaattat ttgaaaaaac
    17581 aattccaact ggtatacaac atgggactgt aacagttatg attgaacatg aaccatttga
    17641 agtgacgact tatagaattg atggaaatta tagtgatggt agacatccag attcaataga
    17701 atttacaaat aacatagtta aggacttgtc aagaagagat tttactatca attcaattgc
    17761 atacaattca aagacaggat tagtcgaccc atttaatggc tatgaagata tacaaaataa
    17821 gtatatacgt tgtgttggaa atccagtaga tagatttgaa gaagatgcac ttagaatgct
    17881 tagagcagtt agattttcag cacaactcaa ttttaaaata gctgaaggta ctaagcaaag
    17941 tattcataaa aaagcagatt tgataaaaaa tgtttctatt gaaagaatcc agacagagtt
    18001 taataaaata ttagtatctg attcatctaa attaaactta ttaaaatcaa caggtttgtt
    18061 gaaatttata attcctgaaa tatgtgaatt agaagatgtt actcaacaca atccatatca
    18121 catctatgat gtacaaaaac atactttaat agctacagaa gtaattgaag atgaactgta
    18181 tttaaaactt acaatgttgt ttcatgattt aggtaaaaaa gtaactaaaa caactgataa
    18241 aaatggtgtt gaccattttt atactcatag tagagaatct gtaaagattg caaagaagat
    18301 tttgaaaaga cttaagtatg ataattatac tattaataaa gttttaatat taatacaata
    18361 tcatgattat agaatagaac ctaaaaggaa gataattaaa aagttattaa ataaacttga
    18421 agatgttgaa ttatttgaag atttaataaa agttaattgg gctgatacac ttgcaaaaaa
    18481 tccaaagtat gcaaaacaaa agatattaaa tcttattgag tgtgaaaaag aatttaaaca
    18541 tataataaat caaaaggagt gctttaatct taaagattta gctattaatg gaaaagattt
    18601 gatatctata ggtataaaac caggtaaaga tataggatat attttaaata agatgcttga
    18661 aatagtcata aataatccag agttaaatga aaaagaaata ctaaaagaaa aagcattaaa
    18721 tatatataca ttttagattc aaatataaaa aatttaatta ctttaaaaaa catagaataa
    18781 aatacttatt tagataaaat aaataatttt tagagaatat tttagaagtt acgattgtat
    18841 gatttttaaa tgcataggca tttactctag gactatagac taatataaaa aaaaactttg
    18901 ggaatactaa aaccataaat ttataaaatt aatattttat atactgtaaa acagtgagga
    18961 gggacagtat gcatactcaa ctacaaaaga ctattggtct ttcagcagct ctttctactg
    19021 ttgtaggtat ggttataggt tcgggagttt tttttaagcc acaagcaata tacactacaa
    19081 ctaatggagc tccaggtcta ggtattatag cgtggctttt aggtggtttt ataacaataa
    19141 ctgctgggct tacagctaca gaaatttcag ctgctatacc aaaaactggt ggtatgatga
    19201 tttatatcga agaaatatat ggagaaaagt taggtttttt aactggatgg atgcaaactg
    19261 tattattttt cccaggtaca tcagcggcat taggagtaat atttgctcaa caagcatctg
    19321 aacttttagg aatgagccca aataatatgg caaatgtact tcctatagca attggagtta
    19381 tactattttt agcgttatta aatataatag gttcatcctt aggaggaaaa gttcagacag
    19441 tagccactat tggaaagatg atacctttaa ttttaataat agtctttgga tttattaaag
    19501 gtcaaagtag tgaagtatta aatccatttg taggagatgg tgtaaatgca tcaaatgcac
    19561 ttggacaagt attaatagct actttgtttg catatgatgg ttggataaat gttggagcta
    19621 tatcaggaga aatgaagtct ccagagaaag atttaccaag agcaatagtt ggtggtttat
    19681 cattagttat ggcagtttat attataataa atatagctta tctttgggta gtaccagcaa
    19741 gtgaattagc aacagttact tccccagcta cattagtagc aacaagattg tttggaaata
    19801 taggtggaaa agtaataact gtgggtatat taatatcagt atttgggaca ttgaatggat
    19861 atttattaac aggttcaagg ataccttata cattagctga aatgggaact ttaccagcat
    19921 ctaaaactct tttaaaagta aattctggag gttctcctgt aaattcaata ctacttataa
    19981 ctgtgttagc ctgtgtatat gcattatctg gtcaatttaa tcttttgaca gacttaacaa
    20041 tattttcaat atgggtattt tatgtattga cttttatagg tgttatgagg cttagaagac
    20101 aaaagccaga tttacacaga ccatataaag tccctttata tccaataata ccaattatag
    20161 ctatattagg tggactgttt gtaataataa atcaaatatt gacttcaaca gtcatatctt
    20221 taggcggaat atttataaca ctattaggtt taccagttta ttattatatg aaaaaaagat
    20281 aactacttaa tttaagttag ataaatttgg gtaaaggtga aaattaactt ttatccaaat
    20341 tttttatact gttatacaat ataagtatta tatattgctt gatgtgctat tttgtttgat
    20401 ttataattta gtaattgatg ttttaagata atttaagaaa tgaggagtga aagatgctag
    20461 gactttatgt acatattcca ttttgtgtga aaaaatgtaa gtattgtgat tttaattctt
    20521 ataaaatgga catagattca aaaaaaagat atatagaaga cttaaaaata gaaatggaat
    20581 tatatagtaa taaattatat aaagataata aatataaaaa taaagaatgc tgtagtctaa
    20641 ataaaaatga caaaataacg agtatatttg tagggggagg tacacctagt atactaacct
    20701 ctgatgaaat aagagaagta tttataagta taaaagaaat gtttgatata gatgagaatg
    20761 cagaaataac tatagagtgt aatcctggga cattaacatt agaaaaatta aaaactatga
    20821 aagagattgg cataaataga cttagtatag ggctacaagc tattcaagaa aagcatttga
    20881 actttatagg aagaatacat acttatgaag aatttgagaa aaactataaa gatgcattaa
    20941 gtgtaggttt taaaaatatt aatatagatt taatgtacag tcttccaaat caaactctat
    21001 gtgattggaa ggaaactctt gaaaaagttg tacacttaaa tccaacacat atatcagctt
    21061 attcccttat actagaagaa ggaactgaac tatacaatat gtatgaaagt aataaatttg
    21121 aacttataga tgaaaatgta gatatagaaa tgtatgagta tacaataaac tacttaaagt
    21181 caaaaggata taatcaatat gaaatatcca actactcaaa agaaggctat aattgtgagc
    21241 ataatatttt atattgggaa tgtgaacatt atatagggat tggagcagga gcttctggct
    21301 atattaatga gaacagatac aataatgtag aatctttaga agattatcat ttaagtttgg
    21361 taaaaagaga aaaaccaata caagaaaatg aaattctttc tgagaaagat atgatagaag
    21421 aaaagatatt tatggggctt agaatgaata aaggcataaa atttgaagat tttaaaaaaa
    21481 aatttggaat agattttaga gagaagtata ataagcaaat agaaatgcta ttagctagaa
    21541 agcttataaa tcagagcttt gaaggtatac aattaactca aaaaggaaga gaaatatcta
    21601 atagtgtatt tatagaattt atggaataat ttttaaggtg ttgacaaaat aaatttataa
    21661 tggtatataa tatatataat ctttttagca ctcgaactta gtgagtgcta acagctagga
    21721 ggtataataa tatggaatta aatgaaagaa aattaaatat cctcaaagct atagttaaag
    21781 attatataga aacagctgaa gctataggtt ctagaacaat atctaaaaga catgatttag
    21841 gtgttagtgc tgctaccata agaaatgaaa tggctgacct tgaagaatta gggtatttaa
    21901 ttcagcctca tacttctgcg ggtagagttc catctgaaaa aggctataag ttatatgtaa
    21961 actctcttat gagtaaaagc gaattggatg ataatgataa gattcttatt gaacaatgta
    22021 tgaatcacaa tataaatcat ataaaagaat tgattcatga aacttctaag ctattatcgc
    22081 aactaacaaa ctatacaact gtagcagtta ctaagagttt aataaatcaa agtgttataa
    22141 agcacataca gttggttgct atgaatgaca ataatatttt acttatagtt gtaacagata
    22201 aaggggactt aaaaaaagct aaccttacaa ctaatgtata tttagatcaa tctaagttaa
    22261 atttgatttc tgataatctt acaaggaagc tcttaggtaa aagtataaca gatttggatg
    22321 ataatctaat tgcttttatt aaatacgaaa taagtgagta ttctgggctt atagatgagc
    22381 ttttaaatgc tttgaactca aatatgaaag aagaagattt ttctctatca ttaaatggag
    22441 ctactaacat atttagttat ccagaattta atgatgtatt aaaagcaaag tcatttttaa
    22501 atatgttgga aaaaaaggaa acaatagctg acataattaa atcaaaggga atacaaaaag
    22561 acaatctaaa tataataatt ggtagtgata atgattgtga actggcacag gattgtagta
    22621 tagttacagc aacttataat gtagatagag acttggttgg gcgaataagc tttataggcc
    22681 ctaccagaat ggattatgct agaatttact ctataataaa ttatatgagt ttattaatta
    22741 atagaaagta aagaaatttt aaattgcgag gtgtaaagtc ttggataaaa aaaatgagca
    22801 acaggaagta agagaagaaa atgatacttc tattaaccaa gaatcggaaa ctcaagtaga
    22861 gttagaagag gaagttgtta atgaagaatg tgaaacatca agtgaaaaaa ctgatgaaaa
    22921 agaagttgat gatgaaaatg taacagatat aaattctaag ttagcagaaa aaaaattgca
    22981 ggatgagttg gatgaattaa atgataaata tcaaagactt caagccgaat atgcaaacta
    23041 cagaagaaga actcaacaag agaaagagac aataggcgta tttgcaaatg aaaaaataat
    23101 aactgaattg ataccagtta tagatagtat ggaaagagcc ttagatgcat gtgaagataa
    23161 ggaagatact atgtataagg gtataagcct tgtacataaa caattaatag acactttagt
    23221 aaaatttgga gtagaagaaa tagaagcaga atctaaggaa tttgacccta atctacatct
    23281 agcagttatg caagaaagtg tagatggagt tgaagcaaat caaatagtaa tggtacttca
    23341 aaaaggatat aagctaggaa ctaaagttgt aagaccttca atggttaaag tttcttgcta
    23401 agtataaatt aaaggttaaa ttatttaacc taatataaaa tattaaaata agtaaaactt
    23461 aattatataa ttactcgagg aggattttag tcatgggaaa aataatagga atagatttag
    23521 gaacaacaaa ttcatgtgtt gcagtgttag aaggtggaga agcacaaata atagcaaata
    23581 gtgaaggtat gagaactact ccatcagtag tagcatttac aaaagatggt gaaagaatag
    23641 ttggagaacc tgcaaaaaga caagcagtta caaatgcaga taaaacaata acttctataa
    23701 aaactcatat gggaactgat tataaagtaa atatagatgg aaaatcatat actccacaag
    23761 aaatatcagc aataatatta caaaaattaa aatcagatgc agaaagttac ttaggacaaa
    23821 cagtaacaga agcagttata acagtaccag cttactttac agatgctcaa agacaagcaa
    23881 ctaaagatgc tggtagaata gcagggcttg atgttaagag aataataaat gagccaacag
    23941 cagcagctct tgcttatggt atggataaat tagaccaaga aaagaaaata ttagtatttg
    24001 acttaggtgg aggaactttt gacgtatcta tacttgaaat aggagatgga acttttgaag
    24061 ttttagctac agctggtaac aatagattag gtggagatga ctttgaccaa atagtaatag
    24121 attatttagc agaggaattt aaaaaagctg aaggtgtaga tttaagaaat gataaaatgg
    24181 ctcttcaaag attaaaagaa gcagcagaaa aggctaagaa agaattatca tcaacaatga
    24241 gttcaaacat aaacttacct ttcataactg ctacagcaga aggaccaaaa cacttaaata
    24301 tagatttatc aagagctaaa tttgaagaat taactagagg tttagttgaa aaaactatgg
    24361 aaccaactaa gacagcttta caagatgcag gactttctac aggtgatata gatgatgtat
    24421 tattagtagg tggttctaca agaataccag cagtacaaga agctgttaag aagtttatag
    24481 gaaaagaacc tcataaaggt ataaacccag atgagtgtgt tgcagcaggt gcttctatac
    24541 aagctggagt tttagcaggg gatgttaaag acttattatt actagatgtt actccattat
    24601 ctcttggtat agaaactatg ggtaatgtaa tgactaagat aatagaaaga aatactacaa
    24661 taccaactaa gaagtctcaa atattctcaa ctgctgctga taaccaaaca gctgttgata
    24721 tacatgttct tcaaggtgaa agaagtatgg cttatgacaa tacaacttta ggaagattcc
    24781 aattaacaga tataccacca gcacaaagag gaatacctca aatagaagtt acttttgata
    24841 tagatgcaaa tggtatagtt aatgtttcag ctaaagattt aggtacagga aaagagcaaa
    24901 aaataactat aacatcaaac actaatttat cagaatcaga aatagagcaa aagataaaag
    24961 aagcagaaat gaatgcagaa gctgacaaac aaaagaaaga aaaaatagaa gcattcaatc
    25021 aagctgaatc tactatatat caaactgaaa aaactttaaa tgaacttgga gataaaataa
    25081 gttcaggtga aaaagaggat atagaaaaag ctatagcaga cttaaaagct gtaaaagata
    25141 atcaagatgc tacagcagaa gaacttaaga aagctacaga tgaagtaatg actaagttcc
    25201 aaaaagtatc tcaagaaatg taccaaaaag cagctcaaga acaacaagcg gcacaaggtg
    25261 cagaacaagc acaagacaat ggacctaaag atgacaatgt agtagatgct gactttaaag
    25321 aagtagatga agataaataa gctatataat atgtaacact aaatgataat tggaacatat
    25381 atgatttgtg aaaatcatat agatgttaaa gatatatgga caatatacat cataaagaat
    25441 ataatatatc atagattata taacttaaag tagaatataa gaattaatga tagcttgtag
    25501 tttattattc tacaagctat cgttttgtaa aatgaaggtg gtgtcaattt ttgagtacaa
    25561 aaagggatta ttatgaagtc ttaggtatca gtaaaggtgc agaagctcaa gagataaaaa
    25621 aagcttatag aaaattagca atgaagtacc atccagatag aaatcctgga gataaagagg
    25681 cggaagaaaa atttaaagag ataaatgaag cttatgaagt tctatctgat gatacaaaga
    25741 gaaaaactta tgaccagttt ggacatgatg ggctaaatgg tcaaggcggt tttggtggtc
    25801 aaggaggatt tggcggtcaa ggctttggtg gttttgaaga tatgttcgga gatatatttg
    25861 gtgacatgtt tggaggaagc ttcggaggtg gaagagcaag aagaagaggg cctcaacgtg
    25921 gtgcagatat aagacagagt gttactatat cttttgaaga agctgcattt ggtaaaaaga
    25981 tgtctataaa agtaaataga agtgaagaat gtgaagaatg taatggaact ggagcaaaac
    26041 caggaacatc aaagaaaact tgttcaactt gtaatggaac tggtcaagtt agaactgttc
    26101 aaagaactcc atttggtaat atagcaagtt caagaccatg tagtgcttgt aatggaactg
    26161 gagaagtgat tgaatctcca tgtagtaaat gtcatggaac tggaaataca agaaaagtta
    26221 agactataga agtagatata cctgctggta tagatgatgg tcaaatgata aaactttctg
    26281 gtcaaggtga agtaggagaa aaaggtgctc caagaggaga cttatatatt gtagttaatg
    26341 ttaagtcaca tccactattt actagagatg gaaatgatat ttattttgaa atgccaatta
    26401 catttgttca agcaacttta ggtgatgaga tagaagtacc tactttggat ggaaaagtta
    26461 aatatagtgt gccagagggg acacaaactg gaactgtatt tagacttaaa gagaaaggaa
    26521 tccctagaat tagaggtaat tcaagaggag accaatatgt taaggttgta gttgaaattc
    26581 ctaaaaaatt aaacgataag caaaaagaac tattgagaga atttgcgaag gagtgtgggt
    26641 cgaacgtcca cgaaaagaag aaaaccttcg gacaaaagat agaagatatg tttaagaaaa
    26701 agtagttaaa gtagccaata tataatcata tttttaaaat cttagttatt tataagattg
    26761 tcaaaggaga tattatgatt tttatattgg ctatttttgt ataaaaaata aatgtgcata
    26821 aaataaaact gaatataaat aattttctga aaaatacttg tcctaaaata tataatatgc
    26881 tataatataa tcaaataaaa gaaaggggtg attccagtgg gaacggatgt ggcactgcca
    26941 aatgatgaag aaaactttat tgtaatatac aaaataaaat tagatacttt tagtgctaga
    27001 ttaagtactg gaaaaactca tgagctggga taattattgt aattacctag actcaaactt
    27061 ttagtagtaa aatagtaata ttatatagac aaggtatcta atttttaaat cttttgtatg
    27121 aattaaaatt tttataaagg gtatatctga ttagaatgta attagggaaa cctagttgtg
    27181 aattactttt agtatataca tgtgtaaaga aatatgtata tggtattggg tattgtgcta
    27241 gatgtgtatg taaaggaata tgtgtctagt tgggtatatg taaagaaata tacttataat
    27301 ctatgaaaaa tttttagtat aaattgctaa aacattgaga ttgtttttta tagaaagtta
    27361 gtttatacca actaaaaatg tgcttaagat ttgtttgatg tttaattata aataaggttg
    27421 tagataaaaa aataagtaaa gacaaaggtt taaaatactt gctttaaata tagtttgttt
    27481 tgaacacttg ccaaattaaa ataattaata aaatagacat cttctcatag taaaatatac
    27541 tgtgagagga tgtttttgct ttgttcataa acttttatga aaaatttgca tagtgtcaca
    27601 ttttaatata ctatatatat aataaaatat agataaaatt aaatgattca aaagagaaag
    27661 gtgaaaagag atgtattata taggtgtaga cataggagga acaggtatac aagcaggagt
    27721 agttgataac tatgggaaga taatttttag gtcagagtgt aaaacagtaa ttgaaaaagg
    27781 atttgaagga atacttaatg acataaaaat aatgatttat aagttgttgg aagataataa
    27841 acttactatg agtgacataa aatctattgg atttggtgtt ccaggtttta taaataaaga
    27901 aggattagta acttgtgtaa atttaaaatg gaataaaaaa gcttttaata aagagttaaa
    27961 aaaaagattt ccagatgttg aaattcatgg agaaaatgat gcaacagtag cagcacttgg
    28021 agaagcaaag tttggaagta tgaaaggtgc taatgttgga gtattatata ctttaggaac
    28081 tggtgtaggt ggaggaatag taattaatca aaaagtcttt tctggagcac atggattggg
    28141 ttcagaaata ggtcatcaaa ttataggaga aaactatttt aattgtaatt gtggaaataa
    28201 tggttgtgta gaaacttttt gttcagcaac tgcaattata aagtattctc aaaaactgat
    28261 agaagaagga gaaaagagta ggattttaga tttggcagag ggaaatttag aaaatgtaaa
    28321 tgcaaagatg gtgtttgatg catatagaga aaatgattta gtggcaatta aagtaataaa
    28381 tagatttaaa gagtatttag caaaaacttt tgcaaacaca ataaattctt tagacccaga
    28441 aattatatct ataggaggag gcatttctaa atctagcgat ataatacttg atggaataga
    28501 aggtttggta agaaaatttg tattatacaa aacagaagat atagctacaa ttacatgtgc
    28561 aactttaggt tcagacgcag gaattatagg agcagctttt ttataatgta aatgaactgc
    28621 ttattggagg cgtgatgata tatgggtaaa acaaatgcta gaagaacgct ttttttgata
    28681 gcaataggtt caggaactat gctgaatcct cttaattctt ctatgatttc acttgcttta
    28741 catagtatac aaaatgattt tcatattagt tttacaactg tatcttggat tgtttcagca
    28801 ttttacctag ctagtgctat tgcacaacca gtttcaggta agataggaga tttaataggt
    28861 agaaaggtct tatttttgag tggtttgatg cttgtaattt tatcagcatc catagtacca
    28921 cttgttcaat cattctttat attgattttt ataagagtaa ttcaagcaat tggaacaagt
    28981 accctatatc catctggagt tgctcttata caaaataata taaaagaaag acaatcttct
    29041 gcattagcag ttctaaccat ttttgcttca actacagcag gattaggtcc gacacttgga
    29101 ggattattgt tggacttagg aggatggcat gctatatttc ttgtaaatat acctgttgtt
    29161 ttggtttcta tatgcttagg gtatttttta ttcccaaaag aagtaaaaga aaagaaatct
    29221 ataaaagaaa ctttaaaaaa tgttttgtca agattagata ttattggcat acttctattt
    29281 tcaattgcaa tgatttttat attactattt ttgctatcta tgaaggaatc ttttaactta
    29341 gaacaacttg tatttggtat aatactgatg tgtcttttta tttggcatga attgagaaca
    29401 aaattgccat ttatagatat taagcttttt gtaagtaatc ctaaattgtc aaaagtttac
    29461 ttgcaattta ttatattaaa tttctttaat tatattttgt tttttggtct tcctagttat
    29521 tttcaagatg ccttacatta tagtgcaaag tcgactggat tatttatgct atttatgtct
    29581 ggtataggta tatttatatc tccattaact ggaaaatgga tagataaatc tggaactaga
    29641 tttcctcttg ttacatcatc aatctttatg tttattagtg cagtacttct ttcagtagtt
    29701 tttatccata tacctgtaat aggaaagggt attatattat cattagcagg tattagttat
    29761 ggagttggta atgtagccct gcaatcctct atgtttgaag aaagtcctcg tgacagtata
    29821 gggacagctt ctggtctgtt tcaaactagt cgttatttag gttctatttt agcaactgtt
    29881 ttacttggaa tggtatttga agtaaatata acatcagaac aatttcagat attaggttat
    29941 gttatggtgg tcttaggtac aataagtttt ttattaaatt ttatattaaa gaaagaatta
    30001 agaaaagtag aatagtggat ttatggattt aataagtcta ataactgata agtttaaaaa
    30061 tattgtttgt aaaacataac tggtttgata tttattaatt ctaagttttg aagcatagag
    30121 tactcgataa gtatacaata ttttgaatta aaattaagca gaaatttgaa ctagtctttc
    30181 aaatctctgc ttaatttagt agttaaattt ttttatagag aaaataagat ttgacactta
    30241 gataaaaaat cattgcattt tcaaaattct ttaaatcaaa cccaagtatc tcattaatct
    30301 tttccaatct atatactagc gtgtttctat gtaaaaacat actagtagat gtcttagaaa
    30361 tatttaaatc atta
//
LOCUS       DBGJFU010000038        30366 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.038, whole genome shotgun sequence.
ACCESSION   DBGJFU010000038 DBGJFU010000000
VERSION     DBGJFU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.038"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(78..929)
                     /locus_tag="ACOIAS_001658"
     CDS             complement(78..929)
                     /locus_tag="ACOIAS_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2701371.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(946..1866)
                     /locus_tag="ACOIAS_001659"
     CDS             complement(946..1866)
                     /locus_tag="ACOIAS_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGZ2701372.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(1976..2890)
                     /gene="murQ"
                     /locus_tag="ACOIAS_001660"
     CDS             complement(1976..2890)
                     /gene="murQ"
                     /locus_tag="ACOIAS_001660"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGZ2701373.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(2989..4086)
                     /locus_tag="ACOIAS_001661"
     CDS             complement(2989..4086)
                     /locus_tag="ACOIAS_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HGZ2701374.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDIGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(4319..5665)
                     /locus_tag="ACOIAS_001662"
     CDS             complement(4319..5665)
                     /locus_tag="ACOIAS_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGZ2701375.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPELLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(5754..6062)
                     /locus_tag="ACOIAS_001663"
     CDS             complement(5754..6062)
                     /locus_tag="ACOIAS_001663"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2701376.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(6324..6722)
                     /locus_tag="ACOIAS_001664"
     CDS             complement(6324..6722)
                     /locus_tag="ACOIAS_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701377.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(6724..7875)
                     /gene="anmK"
                     /locus_tag="ACOIAS_001665"
     CDS             complement(6724..7875)
                     /gene="anmK"
                     /locus_tag="ACOIAS_001665"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase AnmK"
                     /protein_id="HGZ2701378.1"
                     /translation="MYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEIPKD
                     IKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHGQTI
                     FHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEFLLY
                     SDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYDKNG
                     YFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDLIAT
                     VTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQDEYG
                     YSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(8315..9334)
                     /locus_tag="ACOIAS_001666"
     CDS             complement(8315..9334)
                     /locus_tag="ACOIAS_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGZ2701379.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSIDS"
     gene            complement(9328..10008)
                     /locus_tag="ACOIAS_001667"
     CDS             complement(9328..10008)
                     /locus_tag="ACOIAS_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2701380.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLEVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(10160..12067)
                     /locus_tag="ACOIAS_001668"
     CDS             complement(10160..12067)
                     /locus_tag="ACOIAS_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2701381.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(12068..12820)
                     /locus_tag="ACOIAS_001669"
     CDS             complement(12068..12820)
                     /locus_tag="ACOIAS_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701382.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(13136..14290)
                     /locus_tag="ACOIAS_001670"
     CDS             complement(13136..14290)
                     /locus_tag="ACOIAS_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2701383.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(14293..14760)
                     /locus_tag="ACOIAS_001671"
     CDS             complement(14293..14760)
                     /locus_tag="ACOIAS_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2701384.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(14774..15262)
                     /locus_tag="ACOIAS_001672"
     CDS             complement(14774..15262)
                     /locus_tag="ACOIAS_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701385.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(15276..16607)
                     /locus_tag="ACOIAS_001673"
     CDS             complement(15276..16607)
                     /locus_tag="ACOIAS_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGZ2701386.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(16621..18186)
                     /locus_tag="ACOIAS_001674"
     CDS             complement(16621..18186)
                     /locus_tag="ACOIAS_001674"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HGZ2701387.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(18356..19141)
                     /locus_tag="ACOIAS_001675"
     CDS             complement(18356..19141)
                     /locus_tag="ACOIAS_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2701388.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(19308..20507)
                     /locus_tag="ACOIAS_001676"
     CDS             complement(19308..20507)
                     /locus_tag="ACOIAS_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HGZ2701389.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEEMSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(21029..22366)
                     /gene="xylA"
                     /locus_tag="ACOIAS_001677"
     CDS             complement(21029..22366)
                     /gene="xylA"
                     /locus_tag="ACOIAS_001677"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGZ2701390.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(22462..24012)
                     /gene="xylB"
                     /locus_tag="ACOIAS_001678"
     CDS             complement(22462..24012)
                     /gene="xylB"
                     /locus_tag="ACOIAS_001678"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGZ2701391.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            24220..25380
                     /locus_tag="ACOIAS_001679"
     CDS             24220..25380
                     /locus_tag="ACOIAS_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2701392.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(25536..25940)
                     /locus_tag="ACOIAS_001680"
     CDS             complement(25536..25940)
                     /locus_tag="ACOIAS_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2701393.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(25961..26443)
                     /locus_tag="ACOIAS_001681"
     CDS             complement(25961..26443)
                     /locus_tag="ACOIAS_001681"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2701394.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(26463..27272)
                     /locus_tag="ACOIAS_001682"
     CDS             complement(26463..27272)
                     /locus_tag="ACOIAS_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2701395.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(27265..28044)
                     /locus_tag="ACOIAS_001683"
     CDS             complement(27265..28044)
                     /locus_tag="ACOIAS_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2701396.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(28044..30122)
                     /locus_tag="ACOIAS_001684"
     CDS             complement(28044..30122)
                     /locus_tag="ACOIAS_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HGZ2701397.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT         9960 a         5415 c         3164 g        11827 t
ORIGIN      
        1 ttttagatat ttatctattt cttattgact tacgcttata gcctaactta tttgtttagt
       61 tgatatataa cctagtttta agcattatta atatttaaat aatcaagtat cctttttgtt
      121 tcttgtagtt tctgttctgc ctcatttaag ttttcttgaa ttatagaaat atatattata
      181 tctatcaacg taagcattgc tattcttgaa gaaatagctc cttctctaag tcttttttct
      241 atatttggta ctactagctt taaatcacta atcttactaa gatgattgtc actatattta
      301 gttatacaaa tgaccttaca tccttttaat tttgcatttt ctatagactt tattacttct
      361 tttgattctc cagagtaaga tattgctata gctatatctt ctttatctac atttgaagat
      421 accataagtt gtgtatgact atctaaagag gtaaatgcct gtttatttat ccttaaaagt
      481 ttcatctgta aatctagtgc aactaaagcc gaaccaccta ctccaaatat atatacattt
      541 ttagccttta ttatctgttc aactgcttct tttattatat ctttttcatt caacaacctt
      601 gtttggttga tagtctctat gtttttgttt gtaattttgt ttataacact atctatatta
      661 caatcatagt tttcatctac atatgtgtat tcattttctt tgcccatcaa atctttagct
      721 atctcaattt ttaaatcccc aaaacccgaa tatcccattt ttctagaaaa cctaactata
      781 cttgctgtac ttgtttttga gtttgacgcc aattctgata cagaaagctt acttatagcc
      841 tctgtattat taagtatgta atcagctatt tttttatcta cttccgttaa atcttcatat
      901 atctgtttta attttaaaat gcaactcata ctctatcacc tctacctatt tactataata
      961 tttcttctta gcaagtaaac atgctcctaa atctggagtc aacactggtc tcactatacc
     1021 aaatctcgtt ttattcaaat attttttaag tggctctaat attaaatctc ctgacttaaa
     1081 cactccacct gaataagata cctttatctt tgtaccttgt tcaaaatgat gctctaatcc
     1141 tattataagc ctacttaatt cataagctgc atcatcaaat attttactag cagtattatc
     1201 accttcaata actgctttta aacatatcgt agcaaacttt gctatttcta ttctatctcc
     1261 ttgtatttca tcatttacat attttattat gtcataatca ttctctatat taagatgtct
     1321 ttttattaaa tcatataatt ttgtttttga acaccttcca tcagactgca ttgtgaacat
     1381 ttttaaggta gccattccta tataatacgc tgaaccttca tctcctaata catagcccca
     1441 tcctccacat ctgtgaagat tcccatcttt atctaaagcc tgtgctattg aaccagtacc
     1501 tgcaattata tttatacctt tttccccatt taaagaacct gctaatgcta tttctacatc
     1561 attacctaga gtataattta tatgactata tgcatttttt gttgctactt ctaatgcata
     1621 caatacttct tttatttttc catatccagc aagtccaaca aatgcatacg ttatttcttc
     1681 tacatttatc ttagcatctt tacaaacttc ttccaaccca gttattaata atttcgttag
     1741 attatcaaat cctatttgat tataatgaca agttcctttg gttatggttc ctactatatt
     1801 taattcttca tctactaatg taaatgttgt tttcgttcct ccaccatcta cacctaaaaa
     1861 atacatacta tcctcctaca atatctttaa taattctttc aatacttaat atctaaaaat
     1921 aaccttccaa tgctgttttt ttaatattga aaggttatca gtactattat ttattttaca
     1981 atttttctat tgattttaaa gcttcactta tataaccttc ttgtctatct aggatactct
     2041 ttgcactatc cttatctaaa ccacttttta tcataaatat agccaattta acgctattat
     2101 ctgcttcttc taagaatttt tctgcaacat catattttac atttgtagct atcattatta
     2161 ttctttttgc tctttcaacc aacttttcat ttgatgtctt aacatctacc atcaagtttg
     2221 agtatacttt tcccatctta accatagaag ctgttgttaa catattacat accatttttt
     2281 gagctgtacc acttttcatt ctagttgaac ccattattac ttctgcccct actataatgt
     2341 ttattggtaa atccgctttt ttactcattt cactattcgg attcatagtt attgatagta
     2401 ctgcacaccc caattcctta gcatactcca ttgcacctat aacataagga gtccttcctg
     2461 atgcagctat accacatata acatccttag atgtaactcc tctatctatt atatcttgct
     2521 ttccaagttc tttatcatct tcagcacctt ctattgcttt aaatattgct tgatttccac
     2581 cagctattat agcttgaaca agctcaaaat ccactccgta agttggagga cattctgtgg
     2641 catctagtat tcctatccta ccactggtgc ctgctcctat atagaataat ctacctccct
     2701 gcttcattct atctactatt atattaacag cttttgctat actctccttt tgttcttcaa
     2761 cagcataggc tacagtttta tcttcgttat ttattaattc tattatgcct aatgtatctt
     2821 ctttatctat ttgcaatgta ttcttatttc taccttctgt aactaaattt tctaaagttt
     2881 ttattaacat atcatttcct cattttctta atattaaatt tttaaacaca ttttaattcc
     2941 aactttcata attatatttt tataataaat taatattttc ataataaatt attttgttga
     3001 gggtttaaaa ttaaactttt gccatggttt aatataatct aatataaata attcttcttt
     3061 tcttatatat cctactacat tcgatttacc actgtttttc atatcagtca aggctatttg
     3121 taactcccct gcataatgac catattcact actttcaatt actatatctc ctctttttat
     3181 tattttagga gtattaaata gttcaaattt gtatccttta tatttaacac gagattgagt
     3241 agaccttatc agactgtcag atatgtcccc tctattaaaa tgtatttctt caaagagtat
     3301 cttcttctca acttctcgta tattttcctc tattgctaca tcaaatgtaa ccatatcttt
     3361 tctcatagaa cctaatacct ttagctcctc atatgttgga taacaatttg atattattat
     3421 atcatttata ttattcatag ctattatatg ctttacttga atatctatag gtagatttct
     3481 gtgcatctca attgttggaa gcccatcagt tacaggccat ggtccaaatg tagtttcatt
     3541 ttgacttgtt acaaaaactg ctgttcttaa tccatactta ttaaatcttt ctgtacattt
     3601 tataaagtgt tctaaattta atccactata tctatgtgga taaaagttat gacatcctgt
     3661 caagttttct ttatttggtt ggtagtccat tattgtatct atataattag tattattact
     3721 catattgatt tctattttaa gattttgagg attaaaagtc attaatgcct cttcagaacc
     3781 tgtaaatcct atatcaagcc ttattccgtc tgccttcgtc tttttaaaaa aagtcaaatc
     3841 tttgtagctt atttctaatt caccaaatac tctgggattt acatcaagta ttacctcaaa
     3901 acctttaaac ttagcgtatt catttatacg tttaaatttt ttaattatat tttctttagt
     3961 atctttaact gataataaac atgaaaatat acgtgtaaat ccattttcag cagctttatc
     4021 tatataatca tatatttcct tagatgttgt tttttctgga taaattgata ttcctaatct
     4081 tcccatctgt gtcatctcct tatatcctat atgtgtttaa atttatttcg aattacacga
     4141 aactttttta aaaatttggt gaaaaaaagt ttcatatata tttataaaaa aagaaaagtt
     4201 taccttttct ttttttataa acaatcaaca aaatatcttc gttacttatt tcataactgt
     4261 tatactttga aatttatact aatctaatat agtttttaat ttccaatata gtttctaatt
     4321 aataagcttc ttgctcttca actttttctt catgaagcaa tctcttatcg tacatcttga
     4381 caaatggata gtaaattatt attgatatag ctatacataa taagttaaga actgaagctc
     4441 tccaatctcc accagtggct aaatatgccc ctactggtcc tggtaatgtc caaggagcag
     4501 taactactac tctggatact aaattagcac tcatagcaaa ccatgaaact gttgccatta
     4561 ctattggtgc tacaacaaat ggtattatta atattgggtt taatactatt ggtgctccaa
     4621 atattattgg ttcatttata ttgaatattg ctggtattac agttgctttt cctaaagact
     4681 ttccataagc tgattttgct ctaaatgcaa gaagtaaagc taagcctatt gtagctccag
     4741 aacctccaat ccatataaac cattgaaaaa agggttcagc agcaatacct ggaactgctt
     4801 gacccgcagc atatgaagct gtattagcat ctaataattg tatccataaa ggtcttgcca
     4861 atgaacctat tattgataca ccatgtattc caaatgacca aaaaaacgtt attaaaaata
     4921 ttagtataat tactgatggt aatgtgtcag tggctgaaac taatggagtt actaatttag
     4981 ctatgaagcc atgccaatca aaccctagga aataagtaac aatagccatt aaaattatta
     5041 ttaatgcggc tggtgtcaga gcttcaaaag accttgtgac tgatgctgga actgcttctg
     5101 gcatggttat cttgaattta gatttgtcag taaatctata aatctcaaca cctattatag
     5161 atgttacaat agcaacaaac attgaacctc caccaagatt agccattggc atttgccatc
     5221 cttgaggaat tgaattatac caagctaaaa gttctggact cttttcaact atctttaata
     5281 actcaggggt aagaccctct attgcagtag gaactattgt caaaaggaat gtagctgtag
     5341 ctaataaacc tcctgtcaat ccatctaact tatatgactt tgccaaacta taacctatgc
     5401 caaatgtaga atatactgat ataataaaaa atgtcattct atatggtagt aatattgtaa
     5461 ctgcattttc ctttagatac tgagttaccc cccatgaatc tggtaatgga ggaaatgcta
     5521 atatcaagaa aaatgaccca actattataa gtggtaatga agctacgatt ccatctctta
     5581 cagctcttaa gtgacgttgt tctgccaatc tggccataga agttgataat ttagtgtcta
     5641 aaaattctgt aaatttatct agcatctttt ctctccttaa ttaatattta tttttattat
     5701 cattaaaatt tttataaaat caactttctt ctaatctaac aacactgcaa ctactattta
     5761 ctcaacagtt cttgaacttg ttttaataac tttggtcctc ctaatggact ataagcttgt
     5821 gcttgtatta atgcacatgg tacactaacc tcatcagcaa aagcttttaa atagtcaaat
     5881 ctatgtctga cttgtggagc taccatagca atatcccatc catttttaac ttcattctca
     5941 aactcttgag taccaacagc caaaacatct atatctattc cattttttgc tccttctttt
     6001 tgtaatgcat taactgctat agcacttgac atccctgatg aacaaacaaa taatactctc
     6061 atattgattc cccctgtttt tttatttctt ttcaatcatt atagcatgta tttttgatta
     6121 caggaatacg tttgtatata ttttttcata ttttacattt taaattgtaa ttttatttca
     6181 tgcatttata tcttgcttta attgctttat ttatctaatc attcttttgt ctaaaattac
     6241 acactactta aaatactcat atcttttctt aaaaaagaaa aaagactatc ttattattaa
     6301 cataaaatag tctcaatact tttttaaata tgtattcctt tatatatcca gaagtaacct
     6361 attattgcaa atgataaagc aaataaaata tacagtctaa aaaatcccaa agaaaaacct
     6421 ctcttagttt caacaaagta tcttgtatat gcaactgcaa aagatgtaat taatgaattt
     6481 cctataacaa ctccaaatct attatctatt ccaaatatag ataataacaa aggaaaaact
     6541 acagtactta caagagatga aactattagt attaatccac ttttaaaact tatagttgga
     6601 atattataat ctgttctttg atttttctcc atttgtttct tagtattttt attacttttt
     6661 gtactttttg catttttata attattattt ttctttcttt tctttttttt attattagac
     6721 atgtcattta cctccaaaag gtttaggtgt tatattccct aaaataactt tatttttagc
     6781 ccctgttgca gatattacat tagaaaaaga attatttaat gtttcattgc ccaagattac
     6841 aaatgccaaa gcctcctttg catcagaaga atacccatat tcatcttgag ttaatacttc
     6901 aacctctaat aataactctt ttatatatcc tattaaagtt agattatgag ctcctccacc
     6961 tccaattaat aaagtatcta cattaacctt tggaagtatg aagtttttat aattataata
     7021 tatagctttt gccgtaaaca tagttactgt tgctatcaaa tcatatttat ctacatgttt
     7081 atatttgtta agcatgttat ccaaataaac ttgtccaaat acctctcttc cagtgctttt
     7141 aggtggcgct tgactaagat acttatgact catcaaatct tttaacattt cttcattaat
     7201 tttacctttt gatgcaaagt atccattttt atcatacttt ctattaaata atctttgaca
     7261 tactccatct attatcatat ttcctggtcc tgtatcaaat gcaaatacat catctatgtt
     7321 acatgattta ggtattatag taatatttcc tattccacca atattttgaa gtgctaaatt
     7381 tttattgtta tcactatata ataaaaattc actatatggt actaatggag caccttctcc
     7441 acctgctgct atatccatta ctctaaaatt agatattacc tttgtatttg tttcatatgc
     7501 tataactgct ggttcaccta tttgaagcgt tgaagataca aaattattat aacttcttgg
     7561 tatatgaaag attgtttgac catgagatgc gataaaatcc aaatttgcta tatgaaagtt
     7621 tgctttatga cacactgatt ttacagcttt actaaataaa tatccaagct taaaatttaa
     7681 gctacagatt aaatcaacac tcgattcctc ttctatacag cactttctta tttcgtcttt
     7741 tatatcttta ggaatttcta gagttataaa ttctatttgc ttaactttag tatttctacc
     7801 attcccacta atttcagcta gtactgcatc aactccatct aaggaagttc ctgacattaa
     7861 acctacacta tacatagtta tcatcctttt aagtttatag ttattcctcc ccaaatcaag
     7921 gtattaagtt taataatttt agaattaatc ttatttcatc tttaattacg ataatttact
     7981 cttacgaaat taatcgagtt aatcttatac acgtttttta ccttagctaa atcttacatt
     8041 aatttatttt cagtgtcaat gtaataccat gtaatttttt ttcacaaaca aaacatattc
     8101 tctctccaaa aaccatatta aaatagttac tacaaataaa aaacatacca ggtacctatc
     8161 tgatatgttt ttcatatata ataattatta ttttataata tagatggagc atcatttaag
     8221 gcactaatta aatcttcact attttcaaga actaaattta ttcttattgt atatgtcgat
     8281 ttgtatatga tgatagattt tatagtctaa taacctaact atctatgctt aaataatccg
     8341 atatatcata aaagtagata ttaaaaattg tatatgaacc aattttcgaa catacctcaa
     8401 tctcatgccc taaactatct atcattttct ttacaaaata catccccatt ccagtagact
     8461 tatacaattg atttcttcca tttttccccg taaatccctt gtcaaaaacc ctagatatat
     8521 cttcctcatt tatacctata ccaaaatctt ttatagataa taccactcca ttttctaact
     8581 tttttgaatt aaattcaatc ttaccaactt cctttgaata ctttatagca ttattaataa
     8641 tttggtctaa aacatatgac atgcatttag aatctgtgta aacatcattt tctaattcat
     8701 tcaaactaac ttctacatta ttttttataa gcaaaaatga attatttttt atagacattt
     8761 ttaccaattt ttctaaatta aatttattta taaatacatc ttcaaacata gtgttgcttc
     8821 tactactata caaaatagag tttactaaaa aatttatctt tgcaacctga ttcttaatat
     8881 ccaaactatc ttctatgcta cttaacctat ctgtaattat acttaaagaa gatattggaa
     8941 tttttatttc atgaacccat ttagctatgt actcatccaa ttcttttaat gattgtttat
     9001 aagtctctat gtcactatta ttttttattt ttaagttttc tataatatct tttattagat
     9061 tttcttcaaa agattgtcca tcaactaaat aactatctat ttcaccacta ttttctatac
     9121 aattatataa atttgtgtaa ctatttctaa aatttatata atctataaca aaaaatacca
     9181 ttattataaa cagcactaaa aaatctaaat atattaactc tgaatattta tttttaaaaa
     9241 tatctatagc aaataagtag atatttatta tactaactac aactaaaggt agagcaatat
     9301 acttagacct acctttcaaa tattttacta gcattgtatt atatatccta tccccttctt
     9361 agtttttata aaatcatcaa aacccagttc tttaacttta cttctcaatc tattcatatt
     9421 tacagtaagt gcattgtctg tcacaaactc gtcattatcc catagagtca tcataagact
     9481 ttcccttgat acaaccctat tcttattttt taataataat gttaaaattt ttatttcgtt
     9541 tttagtcaac tctatacttt tatccttata cttatgtttt ataatccctt tccccacatc
     9601 aaacatcaaa tcttcacaat acaaaatact attagaatca ctataattat agactcttct
     9661 tattatacca tttattttgg taaccaatat ttctatagaa aatggtttgg taatgtaatc
     9721 atctgctcca ttatttatgc ccattattaa atccatatca gaatctcttg atgataaaaa
     9781 tattaatggt acatttgaaa tagctcttat tttttcgcac cagtaaaatc cattatataa
     9841 tggtaaattt acctctaaaa gtactacatc tggtttttta tctataaatt catctaatac
     9901 tacctcaaaa tcatcaatta attctacttt aaatccccat ttttcaagtg ctaacgcaat
     9961 ctcttttgat aaatcaatat cgtcctctac taataaaatt ctatacattt gtatattctc
    10021 cattatccta atatttcact atgctatgaa ttattaatat cataacatat tttatatgaa
    10081 tttattaccc aaattattta gttaatcaac aaggagactc ttattacaag aatctccttt
    10141 ctctttatta atatatcaac taatttttat acactacact cttaaactta ttaacagata
    10201 ctgctccata tattatgaat attatcaaac atatcactaa acttagtata tatataccaa
    10261 ataaatcggt catcaataaa tctcctgcta atctcaatgc aaaataacta gatatagttg
    10321 cacttaacat tggcaaagta taaaaaatag ctacttcttt aagtatagct ctatttatat
    10381 ctttttctgt agtacctatt tttaacaaaa tcttatattt ctctttatct tcatatgcat
    10441 cactatatat tttcatatat attatacttc cagctacaat tgcaaagact aagaataaaa
    10501 acacaagcac tacatatgca aacttcatcc attgactctc accttgagct ttatatccat
    10561 tttgaatata cccatttttt aaacttaaat tcttttcaat attgttgaac attacttgac
    10621 tattttcttc ttgaaatacc tttgcaccat aaaaatacac ttgttgaccc ttatctctta
    10681 gtttaaaata tgtatcatca tttaccacta taatactttg attaagtcct gtccctagta
    10741 ctggaacttt tacttctccc cttgaaatat tatataactg accatcaagt tttatcttgg
    10801 cttcttttgt agctattgat gctatcgttt gagggctttt taactctatt acattatttc
    10861 cactaactaa tctttcacca tactctttta attctttatc attaccattc actttaagag
    10921 tattacaaaa atcactatat ttcattatag tatctgttgt atcataagct tcttttatat
    10981 ctgttttctt taaaattgtt agttctggat ttactgagta ctttatctta ttagtatctt
    11041 ttattatatt ctctatttgt tttttattca cttcatcttt atccattgaa actatgctaa
    11101 tagtgtacac atttctttgt tcttgggcat tcttatgtat atgcttcata gcaactgaag
    11161 caccaagagt tgatattgtt gtagttataa ttatagctat tatagcataa gttttatagt
    11221 ttttatttaa tcggtagttt atattgttta ttgcaattat attatttcca ttaaataata
    11281 tttttttatt tttgattata atatcaaata taacaggcat aactgatttg aataatccat
    11341 aagtacctac tattattata cctataacag gtagtacact agttatatct ccagttctta
    11401 tattggtaga aagatagtag ccatatccta ttactataat tgatactata gctaatatat
    11461 atatacctat tcctaccttt ggtatttttt cttgtttttt agatatattg attagattta
    11521 ttacactact tctacatatt gttataaaac cttttacagc cattattata aatatagaaa
    11581 taaagattaa agtagcatat agtatagctt ctatactaag tccattagat acctttatat
    11641 taaatccaga taactttatt atcactactt gaaaaaatct agatgttatt atccctataa
    11701 ttattcctgt tatacaagaa cttattccta taagcatatt ttctacaaaa tatatttttc
    11761 ctatagtata taaatctaat cccataaaag acaatattcc aatttcttta gacctattct
    11821 taaaaaatat atttgttgca taccatataa agaaaaataa aaatactgat agtatcatta
    11881 ttacagctac taacactgat agtgtaaatt ttttattttc ctcttgtaac acatttatag
    11941 catcaccata tcttaataat tcaaaattag ttagcacaac tactgaaaaa accatagata
    12001 taaaaaatgc tagataagtt cttatgttac tcttaaaatt attataagca attccaagta
    12061 tactcactta catctcacct cccatagagg ctagcatatc cataattttt ccataaaatt
    12121 cttttctact atttcctttg tttaatttac actttatatt tccatcattt atcatatata
    12181 cttcatttga ataacttgct gttagagggt catgtgtaat cattataata gtagcgttat
    12241 tttctctgtt tattttttct aatgattcta aaagttcata tgctgattta gagtctaaag
    12301 caccagttgg ctcatcagca aatatgactg ttggatttgt aattaatgac ctagctgctg
    12361 cgcctctttg tttttgtcct cctgataact gatatggata tttatctaaa tgattttcta
    12421 atccaaattt tttagctata gaaaatacct tagcttctat ctccttactt ttcttttttc
    12481 ctaatgctag aggaagtgct atattgtcca ttagtttcat attgtctaat agattatagt
    12541 cttgaaatat aaaccctatt ttttctcttc ttaattttga tagttcatta ttttttactt
    12601 ttgatatatt aattccatct agtattattt ctcctgaagt ttgtttgtca agtgtagaca
    12661 ttaaatttag caatgttgtc tttcctgcac cagatggacc cattattgat ataaaatcac
    12721 cttcattgac tgtaagagat atatttttta atacatttgt tttaaatcct tttattccat
    12781 aatcttttga tatatttttt atttttaata tttctttcat aacaatagcc tccagttgct
    12841 aattttttaa tatatattta ttttaaaggt tttatataaa taaatcctta agtaaatatt
    12901 acattttaac tgttagcatt acacttttgt aacatatgga agactattat aattatagta
    12961 gaaatatctt aaaataaaag catgatttac ttctttgtat aaacaaagaa aagaagctcc
    13021 taagaagcct ctaatctata caattattct tctctataaa aaaccaaaat acacattgat
    13081 tgtggcaaat acagatacta taaacaagtg atattactac tatatattaa aacaattatt
    13141 cctgaagata attcaataag actatcaagc aataagctaa gcatccaaaa agacttaata
    13201 ccattaatgt ataattatac ccaatattaa tgcttaaaaa accagcaaca gttggaataa
    13261 tcagagatga acctcccata aatatactta aagctattaa ttggctacta cttctaagtt
    13321 ttttaggaga aacttcaaat attaatcttt ttgaagtaat taaaattaaa ggagtagaaa
    13381 acacttgaaa aacacacagt ataataatga tatttgaatc atttgttaaa gcaaataata
    13441 gaaactgaac tgttatcata atagcactta attttaataa gtttaaactg ctatattttt
    13501 ttaataaata agaaccagcc agatatgttg gtatctctaa caatgactgt aaagaccact
    13561 ttaaagatat ctgagaattt gtagcattta aagctatcat tttatctatt acagtacagt
    13621 tatttgctac caccattgag tataataaga ataaaataca tactagtaag atatattttt
    13681 tatcttttaa taattcaata atatctttat tagttacatt tattttctta ttaatctttt
    13741 caatatcagg taataatagt atatttataa taagtaatac tacaattaaa ccaatcgcta
    13801 cagataatcc aaaatatgag aatctaagaa tcaagtaaga tgctaccata cttccaatag
    13861 cccatccaat cgacccaaaa gtcttaataa atgacaaata attattaata ttatctccac
    13921 aacctattat ccatgtatca tataaactac agcaagtatt taataatcct cctgataaag
    13981 caacaagtaa taagtatatt ataaaagttg cagattcagt agaaaacata ataactgatg
    14041 caattccata aactgataaa ctaattaaaa caattttttt cattgtttga tacttatcag
    14101 ctaatactcc aaataataat tgaaataata tattggttat tgcataactc gataatataa
    14161 ttcctctttc aaacgctgag taacctaatt ttgataagaa aggtgtgaat tttgttgaag
    14221 caaaggcaaa aataatatat gagaaaaaat agaatatgat aaatttaaat ttaatcgctt
    14281 tattaatcat aatcaatcaa aattgataac tttaatacca ctatttcctt cctgatttaa
    14341 gtttgaaaga tgatatttta ccgtattagg attctctaca ataactaaag gaatatataa
    14401 agtattttca agtaacttat tatcaatttc tactaacttt tcccccatat taacttcttg
    14461 accttgttta acttctaatt taaagttttc tttttttgtt gttgcactgt ccaatccaat
    14521 atgaataaga atttgaacat cattttgcaa tttaattcct atagcatggt tagtttgagc
    14581 aataaaactt attactcctt tagtcggtga tttaagaata ttgtcctttg ctatcatagc
    14641 aaatccatct cccattacct tagttgaaaa tactttatca ggaatttctc caagattaat
    14701 tatttttcca tcaatgtatg aattaattgt aattgcttct ttttttcttt taaagaacat
    14761 tatagacact cctctacata attaacttta ttctaattat cattacaaat aacgatatta
    14821 agcatactgc aattgaggaa aaaagagcct tatagctctt tccccattgt agttctttaa
    14881 tactgcttat gatttgttta attccaacaa atttataaat ataggtaaac atgataacaa
    14941 tagttattgt gtaaatttgg aatatctgcc ctaataagac taaatagaaa gcaatcatgt
    15001 tttcttttat ctctttattc ttttttatat tgtaaataac atatgcactg tatactgcac
    15061 taaagttaga gattaaattt aaacttgcat taacatcatt gtttaaaagt tgtgaaaatg
    15121 aaatatttgt aaataatgat gtcaattcta aatacactat atataaaaca ggtaatatta
    15181 gaaaacaaac cataaccact gaaagaaata tattttcatc ttttaatact gctgtgatat
    15241 tttttatttt atttgttatc atgtttttgt tctccttatt taaaagttgg ccagaaatcc
    15301 ttattagcca caatcatttc atctaagatt tcttttgcta cttttgcact tggaacagtt
    15361 ttactcatag taaacgactg ccataatttt tgataacttt tttctatcca tgcttctaca
    15421 gctaattttt cagaagcaac ttgttgttcc attaaacctt tttggaaagt tggaatttta
    15481 cctatagaca taggttcaac catatctttt ccaacaaggc aaggaatttc aaccattgca
    15541 tcatctgcaa aattttcaat agctccatta ttaggaacaa tcagtaaaaa tctttcctga
    15601 gtattaaatg ctaatgcaca agctaattca acaataaatt gagaatgctc acttgcatca
    15661 aaccatgtat cccttgctgt gccatttttt ataattcttt cacattcttc gaatgtatcc
    15721 ttttctcttc tatccataat ttcatttgct cttgtataat ttggattagc atgttcaaca
    15781 gtttcatcag ccattaaata atattttaaa taagtattag gtaatgtagt tggttctatt
    15841 tctaataagt cctttgctgc tctcatagtt tctaaccaac tagattcttt gtgttgacct
    15901 tgaggtgtaa attcctcata tcctttttta gcacaatatt cttgtaattt aggcattaaa
    15961 tcatttcctt ctttgtcctt aatacttgtc caccacccga aatgattcaa tccataatat
    16021 ctaacatcaa agtctttacg agaatttaaa cctaaaactc tagcaaatat ttcctctaaa
    16081 catactggca tatcacaaat attaataatt cttgaatttg gtcttaatac acgacatgct
    16141 tctgcaacta tagctgctgg atttgaataa ttaagcatcc aagcatttgg agaatatttt
    16201 tccatataat caatcatttc gattattgga cctattgttc ttaatccata ggcaattcca
    16261 cctgccccac aagtttcttg accaacaacc ccatatttta gtggaatttt ttcatcttgt
    16321 tcacgtaaag catataaacc aacacgaatt tgtgccatta caaaatcaat atcagtaaat
    16381 gcttcttctg gatttgttgt ataactaaat ttaatttctg gatttttctc tctaacaatt
    16441 attgcacatg cttcagcaac ttttctttgt ctctcatcat tattatcata taatttaatt
    16501 gattcaatag gaaatttagc aacactttca attaacattg ttacaattcc agcagtataa
    16561 gtactacccc caccagcaat tagtatcctc tgtctttttc tttccatctt tccttctcct
    16621 ttataataaa ttttcaaatt cttctcttac acttggtact gaaagcccaa taataatctg
    16681 aatagctttt ccattatgca ctaaaccatg tgcacctact gccttaaaat cttcttcttt
    16741 agaaataatt gagtcatctt tcaaggttaa tctcaacctc gtagcacagt ttgtaacatc
    16801 tacaacattt tctgctccac caactaattt tatgtattca gatgcttgtt gagaggcttt
    16861 actcaattta tttccttttt catctttagt ttctaagtat tcatttttag tatatagttt
    16921 tgtttcttca ctatcttctt ctctaccagg agttttcata tcaaacttct taattaagaa
    16981 agtaaataca aagaaccaga taaatgaaaa tataatccct acaacaactt gaacaacata
    17041 taccttccag tgatacattc caagaggaag ccaatttaat acaacccagt taataattcc
    17101 tccaccaaaa tcaccaacaa ctcctaaggc aaacgaaatc gtttgcaagc acgcatctaa
    17161 aaatgcatgt actaggaata aaagtggtgc tacaaataaa aatgtaaatt ctaacggctc
    17221 agtaattcca gttaatactg cagtaagagt agcaggaatc attaaaccta atactttttt
    17281 ctttttttct ggctttgcag ttacatagaa agcaccaaat acacctaatg aaccaaacac
    17341 cttcgataat ccggataatg aaaatccgat tggatacatt tcctttagag tcttagctga
    17401 agtttgaaaa tcctgaatat gagtagccca atatactgat gtaccacctg gaacaacaac
    17461 actatcataa caaattggtg catatacaaa atgatgtaat ccagttggta ttaaaatctt
    17521 ttgtaagaat gcataacacc aaactcctat atttccacta tttataaaaa acgtttgcaa
    17581 atgtctaata acattttgaa tatgaggcca aataaaacaa gttaataatg caactggtat
    17641 cataacaaag aaacaaattg caactacaag tgatgagcct ctaaataatc caataaaatc
    17701 tggtagttct ttatcataaa atctattatg aatataaatg acaatacctg atattaacaa
    17761 agcaccaacc ataccagtat ctaatgtttt aataccagca atgcttacaa gtccacttcc
    17821 agcacttatt tcagctgcga aatcaactcc aaagcttttt ccccaaaaac ttaacataga
    17881 acctacaaaa taattaaaag tcaaatacgt tgctaaagcc tccatacaag ctcttgcttg
    17941 ttgctttttt gcaagtgcta ttggtaaaga aatagcaaat aataatggta attgattaaa
    18001 taccatccaa gctccactag atattacacc ccaaaattgg taccaaatat tttctggtgt
    18061 agcaagtgaa cccataattg actgattttg aaatacaatt gagaatccaa ccattatccc
    18121 agcaaaagca aataatagtg ttggtgtaaa cattgcccct ccaaaacgct gtattttctt
    18181 catcatataa attactcccc tcctcatatt tttagtttat ctttggcctt agatatctat
    18241 tattttaaca ggttaataaa tatatacaat ctttagggta catttggagc ttattcacag
    18301 tcaaataagt ttttgcacat ttttatgttc aaattctcat aaaaaaaaag aatgactaca
    18361 atttgcaatc aatcccttct gtaatcattc tatgcctttt ataaatacta tatttaataa
    18421 ataataactc aatcaatata aaatataaca ttgtcaattt ataactaggg tgcatttgta
    18481 aaaatgagat attagtcgct gaaatataaa ttgattcatc acttaaatca gtcaatgtat
    18541 tatttttaaa ctctgtaatt gaaattattt tagttcctat taattttaat cttctagcaa
    18601 tattaagtgt attctcattc tcaccactta gggatataat aatcataaca tcatttttac
    18661 tgacaatatt gtaaaaagaa tcatcaaatg ttcttccctc aaaatcatat attatttctt
    18721 gacatgataa gaacattctc tttatctgtt ttgcaactac attttgaata tcacctgaac
    18781 cataagcgta tattgttctt gattcataaa gaagtttaca caatgcatca taatctttat
    18841 ttttttgatc ttcaacatac ttaattattg aattacatgc aatatcaaat gactgatttt
    18901 ctatgacatg aaccttttct gattcccagc ttaataatac tttaaactca ctgaaacctt
    18961 ttaaatctaa ttttttaaca aatcttgtaa tagttgcacg agacacatta catttcatag
    19021 caagttcatt tattgacatc ttactacaat gttttttatt tgcagttata tatttccata
    19081 ttaataaatc tgtatcattc aatttattat aatttaaaaa aatcatttct tctaatctca
    19141 taaactatta ccccctttaa ttaaaaatcg tcataaatat tataccaata ttcaacatta
    19201 aattacagcg tattcttata tttactattt agcccataaa attgactata aaaaatactg
    19261 ctataattga tgtttaaaaa aatgtagttt taattttatt cttttatcta ataattaata
    19321 ttttcaagtt ctagtgcttt tcttatacaa ttatcatctt taaaatcagc agtcctttta
    19381 caatctttta ctttaaattc tggaatagtt ttaaattcct catcacatat tccattctta
    19441 tttaaataca tacaacttgc cattctaact attagaccac tattttttaa attaaacaat
    19501 acagggtcta tacaccctcc atctccgcca gtagtttcac caactaaagt cgcaaattta
    19561 gtttctttac aaaacattgc aaatgactct gatgatgaat atatttttct atcaacaagt
    19621 aagtatatat tacccttaaa ttttgaatca tctgtagatt ttatagtata ggaagtatct
    19681 tcaaaatcat taaaatcttt tataatttct ttaggtgcat tttcctttac acttgttggt
    19741 aaattttcta caggatttaa ttttatattt cttgcatttg tatattgttt tactatttca
    19801 ttattatttc tataaattct ataaccattt attttgacat cattttttat gagtttagat
    19861 acgattccct gccaataact atcacttcca cctaaattac ctcttatatc aataattaaa
    19921 gctttatgtt tttctaaagt gtcaatataa tctccaatta tttttaaatc attatttata
    19981 gagccattct ttgatgacat tgatggtaaa tacatatatc caatctgacc cttaataaca
    20041 tcctttaaaa ttaattcttt ttttacaaaa atatcatctg gcatttttat tttaagattc
    20101 atagagtcat atctatctac aactttttta tcattaagaa aatcatacca atcattatta
    20161 gagtatgatt tcttaaaaag ttcatatctt ttcttattat ctattacttc tgtgtgttta
    20221 ttatttaaat cagataatat agatgacatt tcttctataa attcttcatc attttcagtg
    20281 ttttttattc gttttgtata ttcatcctta tttgctaacc aatcaatatt attaagcctc
    20341 ttatttacac ctagatatgg atatcctgtc tttacaacat tatacacata attaaagtca
    20401 tccacctttt cctcttcaga caactgattc ttttgagaat aagtagaatt acctaataag
    20461 attataaatg ctaaaataat tactaaaaat aatactttct ttttcatgta attctcatcc
    20521 actttcagat tttatatttt aatacattgt atcattattt tattatcacc taaacagtta
    20581 aaagaaattc ttgaagatat attccactat tattctcttt aaaaattttt atttgtattt
    20641 tcaaaattat ttcatgaaag ttataaaata acaattatat tcaaatatct attttactgc
    20701 tatatcctga aggtaaattc ttatcagtga tatactaaaa aagatatgct aaaaaattta
    20761 tccattataa tgacttaaat ttagatactt tactgatttt tatttattag ttgagaaaga
    20821 tattgataat taaaaatatt gataactata gaaatgatta ttttttatag aaattttaag
    20881 gatataaact aattatactt aagtctatat ccttaattta tcatgattat gagaattttt
    20941 acaaatactt atctttgtcc gttttagaat aaaaacaaga acatatcctg ttattttatt
    21001 caaacatcca aatttaatta ctacacaatt acttattaga aattgtatct acttcatata
    21061 tatatctgtt aagtatagct tctagcattt cttgtcttcc agacttattc tttataactg
    21121 gcatttgcag tgcataagat tctaactctt taaatccaac cttaccttct actatatcct
    21181 tacctatacc ttcactaaaa cttgcatatc tttctttagt aaagttttca aatacctcat
    21241 cttctaataa tttatgagct attaataatc cctttgcaaa tgtatccatt cctgcaatat
    21301 aagctaggaa taagtcttct acctcaaaag atgctcttct tactttagag tcaaagttaa
    21361 atcctcctgg tgctattcca ccttgcttta acacttcata catagcaagt gttgcatcat
    21421 atatatttgt tggaaattgg tctgtatccc aacctaataa taggtctcct tgatttgcat
    21481 ctatactacc taaaacattg tttattcttg ctatatttaa ttcatgttgg aaagtatgtc
    21541 ctgcaagtgt tgcatggttt gcttctatat tcattttgaa gtatttatcc agattatact
    21601 ttcttaaaaa tcctaaaaca gtagcagtat caaaatcgta ttgatgttta gtaggctctt
    21661 ttggcttagg ctctattaag aattggccag tgaatcctat ttccttagca taatctacag
    21721 ccatttgtaa gaatcttgca aagttatcca tttcaagttc tgtattagtg ttaagtaaag
    21781 tttcataacc ttctcttcca ccccagaata caaaattttc tcctcctaag tacttagtta
    21841 tttccatagc ttttttaacc tgagctgctg catatgcata tacatctgca ttacatgttg
    21901 tagatgcacc atgaacaaat cttggatttc caaacatatt ggcagttccc cataataact
    21961 ttatacctgt cttttccata ttaactttta tatactcaac tatttcatct aatatcttat
    22021 ttgtctcttc taaatctctt ccttctggtg ctatatctct gtcatgaaaa cagaaatact
    22081 caattcccat cttagacata aattcaaatc ctgcatcaac tttaatcttt gctatctcaa
    22141 ttgggtctgt tatattattc caaggtctct ctactgtgcc tactccaaat gggtcattac
    22201 cctctccaca taaagtatgc caatagctca ttgcaaatct taagtgttct ttcatagtct
    22261 tattacctac cacttgttca ggattgtagt atctaaatgc taattcattg tcagatttta
    22321 ctccttcaaa ttttatctgt cctattcctt taaatatttc actcatttca tgtcctccta
    22381 atattttaca catataatat atgtagtatg ttttcttaat ttaaaactat tttctatctt
    22441 tatttttctt tttaagctaa tttaattatt tatttttgca ttaactactt catctaataa
    22501 cttaaagtca tctttcaaag atttgtatat tcttttatac aatctatgat atatattgta
    22561 tgtatctaca ttttcactta ttggttctaa tttagttgaa gtttttatta tagcatcaca
    22621 tgctttttgt atattttcat atattccagc tcctactaat gcaagaattg caactccaag
    22681 tgctggtcct tcacttgaat ttatagtata tacatcttga ttaaacatat ctgcttgcat
    22741 ctgtctccat actttacttt ttccacctcc acctgaggct cttacttcat taacgtctat
    22801 attaagattc tctattatat ccatacagtc tttaagacta tatgaaacac cttccattat
    22861 tgaccttgca atttcttttt tagaatggat tggagataat ccaaagaaca cacctcttgc
    22921 ataagggtca agatgaggag ttctttctcc catcatatat ggtaagtaaa gcaatccatt
    22981 acatcctgtt ggtacttgac ttacttgatt atttataatc tcatatacat cttcacctaa
    23041 agaatttgaa acttccactt cactttgaca gaaattatct ttaaaccatt ttaatgacaa
    23101 gccagctcct tgagtaactc ccataacatg ccatgtattt ggtattgcat gacagaaagt
    23161 atgaactcta ccttctttat caatttttgg ctcttttgta taagcaaata ctacacctga
    23221 tgtacctata gtagatgaaa ctactccatc tttaactatt ccatttccta tagctcctgc
    23281 tgcttggtct ccagctcctc ctaccactat tgttccttct ttaagccctg tcaaacttgc
    23341 tacatctcta gtcacataac ctgtaatctc ttgtgattca taaactttac ctaaaagatt
    23401 tttatctata tttaactttt ctagtacttc atcgctccaa tctctttttc ttatatcgag
    23461 caactgcata ccacttgcat ctgatacctc tgtagcatat tcacctgtca atttgtatct
    23521 tatataatct tttggaagaa gtattttatt agtattttta tatatatctg gttcattgtt
    23581 tctaacccat aatatctttg atgctgtaaa tcctgtaagt gctggattag cagttatttc
    23641 aatcagtctt tcttcaccta ctaaatcagt tatctcctca cactctttta ctgttcgttg
    23701 gtcacaccat attatagagt ttctcaatgt tttaccttca ttgtccagca taactagacc
    23761 atgcatctgt cctgataatc caattccttt tatacattca ctgcttatat ttgaactttg
    23821 aactacttct cttatagatt cgactacagc tttccaccaa tcatttgcat cctgctctgc
    23881 ccaacctgat ttctcttgta taagaggata ttcatatgta caacttttta tagtatttcc
    23941 tagtttatca aataaaactg ttttagtacc tgatgtacca atatccacac ctaatacata
    24001 ctccaacttc atccctcctt gaatgccact tcataactat cgttttccta tcctaatttt
    24061 tttatatttt aggtatattt tacttgcttt atttttgtta gtatattaaa ctaacttatg
    24121 aactaataat aaaatattta attatctttg tcaatgcaaa ataacctttt atttttgtgt
    24181 aaaaagtatt acaatattat tttaaggagg aatatcaaat tggttactga taaatacact
    24241 attcgagaaa tgaatgaaag attggtactt gaacaaatta taaaaaatgg tcctatttct
    24301 agggcaagta tagcttctac tataggattg aataaagcaa ctatatccgc tattaccaaa
    24361 aaacttattg atgaatctct agttcatgaa atcggaattg gaaatagtac tcatagtggc
    24421 ggaagaaaac ccatattatt agtatttaat aaatgtgcag ggatttcttt aagtatggac
    24481 attggatacg actatatatt ttcttctcta tcttacttag atggaactat tataaattct
    24541 aagaaattaa cagatataca agtaagtaaa gacaatgtta tacaattgat agatgaaatt
    24601 ataaatagtt ataacatatc caaaatagac actccttata aggtaattgg tcttactttg
    24661 gctatacatg ggattacttg tgaaaataaa gtattgttta ctccatatta caatcttaat
    24721 gagattgact tatatagcat ccttagcaaa aagtatgact ttcctattca tatagaaaat
    24781 gaagcaaact taacagcttt agctgagaat actttttcaa ctgtacataa ctcactactt
    24841 agtctcagta ttcacagtgg atttggttca ggcattataa taaataataa attatatagt
    24901 ggcagaaatg gtatgagtgg agaaattggt cacactatca tcatgcctaa cggtaaacta
    24961 tgtccttgtg gaaatcgtgg ctgtttagaa cagtattgtt cagaaaaaaa ggtatttgaa
    25021 cagctttctt ctttagaaaa tattcctaaa atagattctg atatagtaaa gcaactatat
    25081 tatgaagata accataatgc caaaaaagtt attcatgagt tttgtagtta cttaacaata
    25141 gctataaata atgctattac aacttatgct cctgaaataa tatatttaaa tagccaaata
    25201 attagtgata tacctgagat attacagatt acaaaagata tgttagttag ctcttttaac
    25261 aaaggaataa atatagaaat ttcatcatta gggtctgagg catctctata tggtggctct
    25321 gctgtaaata tcaagagctt tttgaatata caaaatttaa ctttaattaa tgaaatataa
    25381 gggttatgac tatatacagc cataaccctt taaattatac attctaaaat tagatttatt
    25441 atatagttta tgcaagatta gctatatttt ttaaaaattg tatatcaatt aagtattaac
    25501 ataagtactt cgttttaaat attaatataa tcttcctact catcatcaga taataaactt
    25561 gcattttgtt caaccataga aatattatct ttacctattt tctctaattt tgctgccaat
    25621 tcctttgtat ctaaaaaagc tgattcaata accataccta aatttagccc tgaaataatt
    25681 ttaattcgac tgtcattatt ggctactaat aaagcagcat tgcatggagt tccacctagt
    25741 aaatctgcca ttataattat ttctgaactt tccttgtaca cttctaaagc tttttttaat
    25801 ttttctattg ttccttctat tccatcatct tgtttcatat ttacagtagg ataatctata
    25861 gaatccccta ttatcatttt tgcagaattt aagacttctt cagccatatt gccatgactc
    25921 atcaaaataa tactattcaa tttaatacac ctctttaaat ctataagatg tctacaagat
    25981 aagttttctt atcatcaggc gtcattatag ttgtaatttc aattccttca tctaaaagtc
    26041 ttttaaatgc ttctttctct tcttcagtaa cactgatatt aggtcttaga actgttgtat
    26101 cttttctact tgacatattt cctacattta tattatgtat tttaaatcct gcctctacaa
    26161 atggaataag gtccacagga gacttaacaa ttaataatac attttgtcct tcatactttc
    26221 ctgcagtaat attttttata gcagtctctt ctgtaattat agatgacgct atacctgcag
    26281 gtgtagccat tcttaaaact tgtttttgaa cagaattgtt agctactgca tcatttacta
    26341 ccattaatct tgtaacaccc aatttattac tccacatagt tgcaacttga ccatggatta
    26401 aacggtcatc tatacgaata tttttaatac ctttcatttc catatctaat atcatccttt
    26461 ccttatttta aaatacctaa tgcatataaa attattgaaa caaccattac tcctaaaatt
    26521 aagtatatag gttttatatt ctttttaaga gcccaataaa caatcacaac tagaattgca
    26581 ggaactaacc caggcataat ttggtctaat atttcttgac ctttcattgt aaaatcacct
    26641 tgttgaaagt tcaaagcaac attagctttt acaactgaag gtattaatgc acctacaacc
    26701 attaaaccta aaatagatgt actctcagtt aaaatcttta aacttttttc aattgaagat
    26761 attagcttag tacctgatgt ataaccagca ttaaatagtg gtatacttaa taatttcaat
    26821 gctacactca ctacaatcca cagtaagatt ccaaatggac ttccatctat cgccatatta
    26881 gctgctatag ctccaaaaat tgtccacgga ataacgacaa ataaactatc tcctaaacct
    26941 gctactggtc ccatcaagcc tgttttaatt gaagcaacag cctcttcagt ctcttcactt
    27001 gataactcct ccattgctac attaataccc aaaatataag gggcaaccca aggattagtg
    27061 ttaaaaaatt gtaaatgatt taatacagca ctttgtaact tttctggatt atctttgtat
    27121 actactttta aaaaaggtag tactgcataa cagtatgcca aggcctgcat cttctcataa
    27181 tttgaagaaa cagaactagt aaaaaaccag cgccataaga cacttttttt caccttttca
    27241 tttgatttat ttaatgatac cttatcactc atcttcgtcc atacctccta ctacagtata
    27301 ttgttgttct tgattttcca aattcttttt gtaaattata agtgcaattg ctccaccaat
    27361 taaagctaca cctaataatg gtactttcat atatactgct aaaacaaaac caattactaa
    27421 gtaactaaaa tactccttag caggaagata tctaagtaat aatcctatcc ctactgctgg
    27481 aagtagtcct ccagctactg ttaaaccacc tgttaaccat tcaggtgtat atttcaaaat
    27541 ggtattaatt aaagaaggtc caaatattac agatagagca actggaatac ctgttgtagc
    27601 tgctgttaat agtactccta acatttgcat ataggttata tttttataat ttcctttttt
    27661 agctccatct tctgctttat gaactagcca tataccaata gtatttctaa gaacatctaa
    27721 ttgtaccatc aataaagcaa ctggaatacc tatagagatt cctacagatg cttcttgctt
    27781 agtcgtaata gttataaatg tggctactat acttgctgtt tgataatcag gtattgatgc
    27841 tccaccaaag ttactaatac ctaatgataa tagctgtaat gtacctccaa tatataaccc
    27901 tgtatttata tctcctaaga taagaccagt aataaaacct gctgttactg gttgatatgt
    27961 tccaaacatt gttgaatttt tttcatagtt tataaaaaat ccatatagta cgattaataa
    28021 taattgccat aattgtggct ccattatgat ttcacctctc ccaaaatatt ttttagttct
    28081 ttaaaattat aactggaatc ttgctttagg aaaataggaa tttcttccag tgggcattca
    28141 acttcataag ttttcccacc ttcataacta actccactaa atatattttc ccatgtatgt
    28201 ccttttggta aatatacttt tctacttcta tctttataat tcattattgg agctaccaaa
    28261 atttcatctc caagcatata tgtattatcc acctcccaag ttttatcatc ttcaggaaat
    28321 tcataaaata aagttctcat aactggtgtt ccatcttcat gcgcttcttt catcagtttt
    28381 tttaaataag tttttaagct ctctctaatt tttatgaatt ttgtaagaat tacttcaact
    28441 tcttcaccat aagaccatat ttcattagga gctccagtta ccattgagcc tccaccttta
    28501 tttgataatg gtttactatg aggcaatcta tctccatgca ttctcaaaat tggtgaaaaa
    28561 gtagcatatt ggaaccatct taccataagt tctctaaatt ctgggtcttt aggatttcct
    28621 ccatggaaac ctccaatatc agtagtccac catggtatac ctgccagtcc catatttaac
    28681 cctgtattta cttgattatt aaaagcttca aaactagaat caatgtcacc agaccatact
    28741 aaagctccat acttttgagc tcctgcccaa gctcctctta caagagttac aacagactct
    28801 atgtcattat gtaaaccatc ataaatcatt tttaaataat caataggata aatattacca
    28861 caacttaaaa catttccttt tttatatcta tagttatcaa aatcatacac agaatagcca
    28921 ggttcagcta catctaacca ataatagtca atcccaagtt gtttataatt tttatctatt
    28981 ctatcccaaa catattctct tgcattttca tttgtcatat ctacaaagac tgtatttcct
    29041 tgaatttgca ttgtcattct aacaccacga ttaacattga ctaaatagcc attttctaaa
    29101 taatcattgt agttttctgc atcactttga actgtaggcc atacagatac tattggttct
    29161 actgaatact cttctttcaa ttttttaact aattctttag gatttttcca ataatctaag
    29221 tcaaatttat attcaccttg cttaggccaa tgaaaatagt caattgcaat tgatgaaagc
    29281 ttaattcctc ttttagaata ttccttcaca acatctaaaa cttcttctga agttctatat
    29341 cttagtttac tttgccatag tcctaaaagg ttttcaggca tcataggaac agttccagta
    29401 acttgagaat aatttttatt taactctttt gggctttctc cacaagtaat ccaataatct
    29461 ataaaatttg tagaaaacat cttccattct gtcatatttt tagcaaaaga tacttttcca
    29521 attcccggat tattccataa aagtccataa cctagactag aaatataaaa aggaactgat
    29581 atttgagaat ttctttgggc taactccaaa acagtattct ttaaatctaa aaatttatgt
    29641 tgatattgtc ccattccaaa aatcttttca gaaggctcag attcaaatct agtagttact
    29701 tcaaattctc cactatagct tggtcgatat tctcttgatt ttaactttag agtagagtta
    29761 aagtctttgg taatctcaat tgtccctaca tcttctccac catcatcatg ttttacagct
    29821 cttaatcgaa tatattcttt caataatatt tcatttttat cattataaaa agtaattcta
    29881 tctctatggt ctaagacagc ttttatcttt ccatttttaa tgattgcact tccatcttca
    29941 tttttattaa ttgtaatatc atctttattt aacttaggtt tttcgttaag agcatagttt
    30001 tcatcaacaa aattttggtc tacaaacgac ctaatacgta aagagttttt tccccatgct
    30061 tctattcgca atagttcatt gtcaaatctt ttttcaatat agtcttcaga tatttttatc
    30121 acggctacct cctaaatcaa tggtataata ttctatcttt gtgtagctta agttaaataa
    30181 tgtcgtttag tatcgttatt tataaattag gttttatata taattcattt gtacattaaa
    30241 ctaacttacc aataatatac tttatttgac tttgtttgtc aatcgctttc cggattttag
    30301 taaaattttc taatatattg ctggaaagtc gttcttaata tcttttattt atacttttaa
    30361 tatctg
//
LOCUS       DBGJFU010000039        30241 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.039, whole genome shotgun sequence.
ACCESSION   DBGJFU010000039 DBGJFU010000000
VERSION     DBGJFU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.039"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            94..996
                     /locus_tag="ACOIAS_001685"
     CDS             94..996
                     /locus_tag="ACOIAS_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2701398.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
     gene            complement(1195..1521)
                     /locus_tag="ACOIAS_001686"
     CDS             complement(1195..1521)
                     /locus_tag="ACOIAS_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701399.1"
                     /translation="MSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVAVWK
                     PSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYSCDL
                     QQNKTT"
     gene            1537..1689
                     /locus_tag="ACOIAS_001687"
     CDS             1537..1689
                     /locus_tag="ACOIAS_001687"
                     /inference="COORDINATES: protein motif:HMM:NF024055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2701400.1"
                     /translation="MPIYVRERYEKMLAVSIRAPNLEGMVENFDKDLSRPNETDLIEL
                     KNAISK"
     gene            1782..4445
                     /gene="mgtA"
                     /locus_tag="ACOIAS_001688"
     CDS             1782..4445
                     /gene="mgtA"
                     /locus_tag="ACOIAS_001688"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGZ2701401.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            4461..4964
                     /locus_tag="ACOIAS_001689"
     CDS             4461..4964
                     /locus_tag="ACOIAS_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HGZ2701402.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            5102..5779
                     /locus_tag="ACOIAS_001690"
     CDS             5102..5779
                     /locus_tag="ACOIAS_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGZ2701403.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            6026..6337
                     /locus_tag="ACOIAS_001691"
     CDS             6026..6337
                     /locus_tag="ACOIAS_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HGZ2701404.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            6503..9268
                     /gene="mgtA"
                     /locus_tag="ACOIAS_001692"
     CDS             6503..9268
                     /gene="mgtA"
                     /locus_tag="ACOIAS_001692"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGZ2701405.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            9281..9448
                     /locus_tag="ACOIAS_001693"
     CDS             9281..9448
                     /locus_tag="ACOIAS_001693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701406.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            10499..10798
                     /locus_tag="ACOIAS_001694"
     CDS             10499..10798
                     /locus_tag="ACOIAS_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2701407.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKRGRSLFALATFAGKGVRL"
     gene            10795..11211
                     /locus_tag="ACOIAS_001695"
     CDS             10795..11211
                     /locus_tag="ACOIAS_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HGZ2701408.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            11216..11455
                     /locus_tag="ACOIAS_001696"
     CDS             11216..11455
                     /locus_tag="ACOIAS_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2701409.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            11774..11917
                     /locus_tag="ACOIAS_001697"
     CDS             11774..11917
                     /locus_tag="ACOIAS_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HGZ2701410.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            11980..13740
                     /locus_tag="ACOIAS_001698"
     CDS             11980..13740
                     /locus_tag="ACOIAS_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGZ2701411.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(14229..14867)
                     /locus_tag="ACOIAS_001699"
     CDS             complement(14229..14867)
                     /locus_tag="ACOIAS_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701412.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(15277..15453)
                     /locus_tag="ACOIAS_001700"
     CDS             complement(15277..15453)
                     /locus_tag="ACOIAS_001700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701413.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(15507..15603)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16400..16801
                     /locus_tag="ACOIAS_001701"
     CDS             16400..16801
                     /locus_tag="ACOIAS_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HGZ2701414.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            16864..17865
                     /gene="rsgA"
                     /locus_tag="ACOIAS_001702"
     CDS             16864..17865
                     /gene="rsgA"
                     /locus_tag="ACOIAS_001702"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGZ2701415.1"
                     /translation="MEYHDLYLARIISQEKNFYQAVCVNGVLFAEVSGKLRFEATIQS
                     DFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRKAAGKVNEEQVVAANIDIVFLCM
                     SLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCDNVHRKLQVVNSIALGVDVVVTS
                     SIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINCLMGGEFLNTKEIRIDDDKGRHA
                     TTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKSFADIEAYFGKCHFRNCTHTSEP
                     GCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSEYLAKKEKKFKNISKINKKIRR"
     gene            18401..19099
                     /locus_tag="ACOIAS_001703"
     CDS             18401..19099
                     /locus_tag="ACOIAS_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701416.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            19277..20167
                     /locus_tag="ACOIAS_001704"
     CDS             19277..20167
                     /locus_tag="ACOIAS_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701417.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(20560..21516)
                     /locus_tag="ACOIAS_001705"
     CDS             complement(20560..21516)
                     /locus_tag="ACOIAS_001705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2701418.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            22219..23214
                     /locus_tag="ACOIAS_001706"
     CDS             22219..23214
                     /locus_tag="ACOIAS_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2701419.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            23201..24055
                     /locus_tag="ACOIAS_001707"
     CDS             23201..24055
                     /locus_tag="ACOIAS_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701420.1"
                     /translation="MSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFDVVV
                     NMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEIC
                     NRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNP
                     VQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFLKMIIENIVEFITGTMIPLVLL
                     PETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMR
                     TLFDGVGI"
     gene            24052..24846
                     /locus_tag="ACOIAS_001708"
     CDS             24052..24846
                     /locus_tag="ACOIAS_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HGZ2701421.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            25131..26510
                     /locus_tag="ACOIAS_001709"
     CDS             25131..26510
                     /locus_tag="ACOIAS_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2701422.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
     gene            26958..27671
                     /locus_tag="ACOIAS_001710"
     CDS             26958..27671
                     /locus_tag="ACOIAS_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2701423.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(27943..29481)
                     /locus_tag="ACOIAS_001711"
     CDS             complement(27943..29481)
                     /locus_tag="ACOIAS_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2701424.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT        10197 a         4200 c         5542 g        10302 t
ORIGIN      
        1 acccgatagg atatacagac ctaagtagta gttactggca acagcactta atcggtgcag
       61 gacgagtaaa acaatagaaa ggacttgaaa aatatgttta agaagaataa gaaacagaca
      121 gaaaatgtca aagaaaaaaa ggtacgtact gtcaaggtag gcacacataa gaaaaccgtg
      181 attgcattgt gggtggtgct tattgcaagc gtgagctttg gagtatataa gaattttacc
      241 gctattgacc agcacacgac ccatgaaaaa gaagtgattg agcttcgctt gcaggacacc
      301 aacgggatag aaaatttcgt gaagaatttt gcaaagtcct attacacatg gagtaacagc
      361 aaagaagcga ttgacgcaag gacgcaggca atcagcggtt atctgacaaa ggaattgcag
      421 gacttgaata tggataccat tagaacggat ataccgacca gctctgccat tacagatgtg
      481 attgtatgga gcattgaaca gtctggaacg gacacttttt ccgctaccta cgaagtagat
      541 cagcagataa aagaggggga acagacaaca gcggttaagg caacctatac cgtaaaagtt
      601 catgtggacg ctggcgggaa tatggtaatt attcagaacc ctacccttgc accagcaatc
      661 gagaaatcag actatgaacc aaagacacca gaagcagacg caagtgtaga cgcggatacc
      721 ataaatgacg ctactgcatt tctggaaact ttctttaagc tatacccaac agccacagaa
      781 aaggagcttg cttactatgt gtctggaaat gtgcttgagt ctatcggcag ggactacctc
      841 tattctgaat tggtaaaccc tatctttacg gttgatgggg acaatgtgaa agtcaaggtt
      901 gccgtgaaat tcattgataa tcagacaaag gcaacacagg tatcgcagtt tgaattagtg
      961 ctacataagg agagcaattg gaagattata ggataattat tgaaagcgtg catttcttta
     1021 ggaatttgca cgcttttgtt aagagcatta ttctttatat ttatgaaaga catcttaaac
     1081 aaagtattaa tggcttcgca aaactttttt cttaatatat ttggattaga aaaagcgcat
     1141 cttaattgta gacttactat acatatgaat tatgccagtc aacgtattta aaatttatgt
     1201 tgttttattt tgttgtaaat cgcaagaata gataagagta atcaatggtg aaggggaatt
     1261 attaaaaata ctattggttt cttctgtccc gttccaattt gcgcattgta ttttttcata
     1321 aattacagag tggtcaatta tgtagttcag atattccata caaactaatt gtattgtttt
     1381 tacactcggt ttccatactg ccacttttgt cattctatca ctcataatac aaatattatt
     1441 atgagcaact tttctaagta gttcatttaa atttgataca tggtcaaggt gaaaaccaag
     1501 aagaagtttg tcagcactca tatgtgtcaa ggggaaatgc ctatttatgt gcgagagcgc
     1561 tatgagaaaa tgcttgccgt atccatccgt gctccaaatt tagaaggaat ggttgaaaac
     1621 tttgataagg atttatctcg ccctaacgag acggatttga tcgagctgaa aaatgccata
     1681 tcaaagtaaa ttgcaagaaa aaagcaagaa tataaatata gcattttcag aacactgtgt
     1741 taaaatgtta ttacttatac ttataaagga ggggatgttt cgtgtcaaaa gcaacactat
     1801 ttgatagtcg tatcaaaaaa tacgcatact gtagaacatc agaaatttat cgagacattg
     1861 gctcatctcc ggatggttta tccattgagc aaatcggttc aatgcgggag aaatacggag
     1921 cgaacagctt caatgggcgg aaaaatgata cgacgatgca acggttgaga cgggcattta
     1981 ttaacccgtt tcatgtgatt ctttttgttc tgggcattgt ttctcttgtt acggatgttt
     2041 tcgtagcatc caattttgcg agaaatgcta ctaccgccat tatcatcttt tccatgatcg
     2101 taattagtgg cgtaattcgt ctgattcagg agctgcgagc aaagagcgct gctgctcagc
     2161 ttgatcgcct agttcatgaa agcgtcacag tgaggcgtga tggaaagtta atagagattc
     2221 ctggcgaaga attagtggta ggggatattg tactattctc tgcaggtgat cgtgttccgg
     2281 cggatatcag attgacgaag attacggatc tctttatctc tcaggctgcg attactggcg
     2341 agagcgccat cattgaaaag agctgccgaa aactcagtta cggagagcag gatacactga
     2401 ctcagcttga aaacctcgct tttatggcaa ccacagtcat cagtggaaaa ggggaaggca
     2461 ttgtactggc agtaggcaaa gataccctgt atggaggatt taccaaaccg gattctgatg
     2521 ataaaaattc ctttaaaaaa ggagccgatt ccatcgcctg ggtgatgata cgttttatgg
     2581 cgatattagt tcccattgta tttatattac tggggattac tgatggaaaa tggatggaat
     2641 cttttgcttt cgccctgtca gttgctgtag gtctgatgcc ggaaatgctt cctatggtta
     2701 ttacagcctg ccttgccaaa ggaagtcttg ctatgagcaa aaagcagacc attatcaagg
     2761 atattaatgc catgcagagc tttggtagca tggatgtgct ttgtatggat aaaacaggaa
     2821 cacttaccaa tgaaagcatc ctgctggaat attatatgga tatttttggg aatgaaaatg
     2881 cagaggttct tgatcttgct tatctgaata gctgctatca ttctggtgtc tgcaatacca
     2941 ttgataatgc cattcttgcc tgcaatacca tgccagggcg ggaagcccat tacgcaaatc
     3001 ttctgacaaa attccaaaaa gctgatgaaa ttccctttga ctatgctcgt aagtttgtca
     3061 gtacacttgt acaggatagc aatgggaata gtcagcttat tatgaaaggt gatattgaac
     3121 atatattctc acgctgcaac catgtagaat atcgtggaaa acttctcccc atggggcagg
     3181 atgcgagaca gagtgtatct tctgtagtag gtgaaatgtt acaggacgga atgaaggtca
     3241 ttgcggtggc acgaaaaaat gtgggagcgc aaaagaaaat aactcctgat gacgaatggg
     3301 gcatgactct agttggctat ctatccttct ttgatgcacc aaagcagaca gcaaaggagt
     3361 ctgtaactgc actcaaaaga ttaaaagtta tccctaagat tctgacaggg gaccaggcag
     3421 cgattgctgt gtctgtttgc cgccgggtgg gtatttatac agagcgtatc ttaactggta
     3481 ctcaactgga tgaaatgaca gactgtgaac ttaaaaaggc tgtggaacat actcatgttt
     3541 ttgcagagct tactccggga cataaggtgc gtcttgtctc tgctatgcag gataatggtc
     3601 attccgtggg atttcttggt gatggagtca acgacattcc ggcactgaat gaagcaaatg
     3661 tgggaatctc tgtagatacg gcagtggacg cagcaaaaga tgctgccgat gtggttttgc
     3721 tgcaaaagga cttgaatgtt ttggagcaag gtgtaatgga ggggcgaaaa acttttacca
     3781 atatgctgaa atatattaaa atcaccgcta gctctaactt tggaaatatt ttttccatca
     3841 tttgtgccag tgccttttta ccgttcctgc ctatgacttc aattcagatt ctgcttttga
     3901 atctgctgta tgatacgttg tgtattgtgc ttccttggga caatgtagat gaggaagaga
     3961 tgctgtcccc aagagactgg tccggcaaaa cactgagaca gtttatgctg tctttcggac
     4021 cgatcagttc tctgtttgat attgtcacct tcctattttt gtattatttc ctatgtccga
     4081 tgctatgtgg aggggcaacc tatctgcagc ttacagatcc ttccctgcaa cttcagtatg
     4141 tagcactgtt tcagactgga tggtttttgg aatctatgtg gacacaggtt ttgattttac
     4201 attttctgcg aacttccaag attccttttt tccagagcag ggcatctgca ccggtcattt
     4261 ctataactct agtggggatt attgctttta cttcccttac atttgtcggc ggtgcgtcgt
     4321 tgtttggact gacgaaatta cctctttggt atttcgtatt tttactgctg gtggtatctc
     4381 tctatatgct gctgaattca gtagccaaat atttttataa gaaaaaatat caagagttga
     4441 tttaagagga ggcattcatg atgaaaaaga atattagtta tattccgatg gattcggctc
     4501 ttgaaatctg gctattagaa aaactactaa gagatccacc ggacagtgat tgtgcgaagg
     4561 atttactttc tggtattcag gagatgattc gtggagaggt atcttcgtta ttgttaggag
     4621 catcagaaga aagcgattgg ccagtgggta ctctgtcaga tagaatcctg gcaggggatc
     4681 tggaaattta tccaagaggc agaaaagtac tgataaaagg ttctgaagtc aacctgactc
     4741 caaaggaatt tgatattttg tactttcttg tccagaacaa aggtgaagtt tttacaaagg
     4801 aacagattta tcgtgccgta tgggaggaag attatattct ggataacagc aacattatgg
     4861 cttttatccg gaagttgcga aaaaagattg aacctgctcc ggattgtccc acatatattt
     4921 tgaccatctg gggtattgga tacaaattta atgatcagat atgacaaaga cggactaaga
     4981 ccattttcgg tttcagtccg tctattttct taccaaatcg caagaaaatc gcaagacttt
     5041 agccatagga ttgtatttgg attttttata tactgttagt agttaaaggg aggtgtttca
     5101 tatgttttac acagatttta ttttgagaat tgccctctcc ttaacacttg gatttcttat
     5161 tggattagaa cggcaattga ctggtcatcc tgcaggtatt cgtatcaatg tgttaatctg
     5221 catgggtaca agtttcttta ccttgtttcc tatgctctac ggttcggatc aggtattccg
     5281 tgtcggaagc agtattatat ccggtgttgg ttttttatgc agtggtgtta ttttcaagga
     5341 cagagggaca gtacgtggaa tgaataccgc tgccacctta tggtgtactg cagcgattgg
     5401 aattcttgca agtacgggaa tgtatgcaat ggcgatcacc gccgctacta ttttaattgg
     5461 ttccaacctg attctacgtc cctttgcaag aaagtttcac ccaattactt ccagtgatga
     5521 atctgaaaag cagtatcgta tttcggttac atgtcaggaa aaggcagagc aggatatacg
     5581 cttattgctg attaacagca attcctgtaa gacgctcttt ttgaacaatc tggaaagcgg
     5641 tgatgtggtt ggtgacaagg ttgagattgt cgcagaatac tgttctgtcg gaaaatccaa
     5701 aaacaatatt ttagaaggaa tcgttgggca ggtattggta attccggaag tgattagtgc
     5761 tggttgggag gtgttgtgag tgaggagaga aatcgaagat ttgaaacgag caattctttt
     5821 tgctttctac cctgtattta caatcttatg tagctatttg ggttggaatt tctttgaaca
     5881 aagaatggta atctttaaga tatggtatca ggtgagtatt gtgatctggt ttattcttgc
     5941 tgttactatt attgtgctct tcattaaact tctgaagctg acaaaactaa gaaacaggtt
     6001 taagcattga aaggaggaac aaacgatgga ttttacatgt atcttttttg gaattttatt
     6061 tactcttgca ggcttttggt tggcctttgg aaaaggatat cgtcatcttt ccctgtggaa
     6121 aaatatgccg caggaggaga aagataaaat taatattgtc ccgctttgtc gtaatgttgg
     6181 agaaatgatt gcgttaaatg ggattatatt cttaatgaaa ggatttttgc cgggattttc
     6241 aaaccactgg tttgtatttg caataatcgc atggctgatt gttgccggct ttgatgtctg
     6301 gtatatcgaa aaaagtgtcc gctatcgcaa ccaataatcc acattgaggt ttcacataga
     6361 tattttttta agattgtgag gtgaaagaca tggactctga tgtcagtcca cgaaggcaga
     6421 gaatattata tgagaatttg gagattccgt gtcgctcata taatattcct tgcccctatg
     6481 aaaattcaag gaggtaagac caatgaacaa aaaagaaaat cgtatggcta ttcgtcagac
     6541 tgtagaaaaa gcagtcatcc gtgacgaaca gaaccgccgc attcaatttg cggcaaccaa
     6601 tcctattaaa gaagttttaa aaaaacttca taccacactt cgtggcttag aagtagatga
     6661 agtatccgta agtcgtgcta aatatggtac caataaagtc actcatgaaa aaaagaaatc
     6721 tctggcaaaa cgactggttg gcgcatttat caatccattt accgccattt tattttgtct
     6781 ggctttagta tccacaatta cagatatgat ttttccgtat ttttctctgt ttggcagtgt
     6841 gccagaggac tttgattccc tgactgttgt aatcatttta acaatggtat tcatttccgg
     6901 tacgcttcgt tttgtacagg aatccagaag cggaaatgca gcagagaaat tgttagccat
     6961 gattacgacc acttgtacag ttactcgtag aaactatgaa aaaaaagaac ttcctatgga
     7021 tgatttggtg gtgggagata ttgtgcatct ttctgctgga gatatgattc cggcggatgt
     7081 tcgtattttg gatgcgaagg atttgtttgt cagtcaggca agtcttactg gtgagagtga
     7141 acctattgaa aaaacaccca atgtaagtgc tcagaaagaa agcgtgactg attacacaaa
     7201 cattgctttt atggggagta atgtaatttc tggtagcgca acagctgttg ttgtttgtgt
     7261 tggcgatcat actctgtttg gctctatggc gtccgcagtt gctggagaag cggttgaaac
     7321 aagctttacc aagggtgtca atgccgtttc ttgggtattg attcgtttta tgctcattat
     7381 ggttccgctg gtgttctttg tcaatggtat caccaagggc gattggttgg cggccttctt
     7441 attcggcgtt tctattgctg ttggtttgac accagaaatg ttgccgatga ttgtaacaac
     7501 ctgtcttgcg aaaggcgctg tttccatgag caagaagcag acaatcgtta aaaacctcaa
     7561 ttccattcag aacttcggtg ctattgatat tctctgtact gacaagactg gcacgctgac
     7621 ccaggataag gttgtgctgg aataccatct gaatgtaaat ggcgaggacg atacgcgagt
     7681 actgcgtcac gcttacctca atagttactt ccagactggc tataaaaacc tgatggattt
     7741 ggcaattatc cataagacgg aggaagagga agccgcagat tcgcgtctca ttgacctgtc
     7801 tgaaaactat gtgaaagtag atgagattcc ttttgacttc acacgtcgtc gtctgactac
     7861 agtggtacag gataataagg gtaagaccca gatggtaaca aaaggtgctg tggaagaaat
     7921 gctctctatt tgtgcgtttg cagagtgtaa tggaggagtg cagcctctga ctgatgacgt
     7981 tcgttacaga attctggaga cagtggacaa tcttaacgat aagggattcc gcgtactagc
     8041 tattgcacag aagagtaatc cttccccaat cggcgcattt ggtgttaaag atgagtgtga
     8101 tatggtactg attggatatc tggcattcct tgatcctcca aaagaatcta ctgcggatgc
     8161 tattaaagct ctgaaagacc atggtgttat aaccaaaatt ttgacaggtg acaacgacaa
     8221 agtgactcga actatctgca agcaggtggg attagaggtt cgcaatatgc ttctcggttc
     8281 tgatttggaa caaatgggcg atgcagagct tgcaagagtg gcagagtcta cagatgtatt
     8341 tgcaaagcta acccctgatc agaaagcccg tgtggtttct gttctccgtg aaaatggtca
     8401 tacggtcggt ttcatgggtg acggtattaa tgatgctgca gctatgaaag ccgccgatat
     8461 tggtatttcg gtggataccg ccgtggacgt ggcaaaagaa tctgctgaca ttattctatt
     8521 ggaaaaagat ctgatggttt tggaacaggg tattattgag ggacgaaaga catatgccaa
     8581 catgatcaag tacattaaaa tgactgcttc atccaacttc ggcaatatgt tctcagtgct
     8641 tgctgcatct gctttgctac ctttcttacc aatgatgagt gttcatctta tatttctaaa
     8701 cttaatttat gatttatcct gtactgcaat tccttgggat aatgtggatg aggagtttat
     8761 taccaaacct cgtaaatggg acgcttccag tgttggaagt tttatgatct ggattggacc
     8821 aaccagttct atctttgatt ttacaaccta tatttttatg tactttgtat tttgtccatt
     8881 gttcgtatcg aatggtgtat tatttaatga tttgcctgct catttcagcg gtgcagaact
     8941 ggcaatgctt caggcacagt atatcgggat gttccaagca gggtggttcg tggaatctat
     9001 gtggagccag acattagtca ttcacatgat tcgtactcca aagctacctt ttgttcagag
     9061 tcgtgcatca gctccggtga ctttgctaac aatgactgga attatagttc tgaccattat
     9121 tccgtttact gtgtttggaa ctatgcttgg atttgttgca ttgcctgcag catactttgc
     9181 ttatctgatt ccatgtattc tgctgtatat gactttggca acaagtttaa aaaaagccta
     9241 tgtccgtcat ttcggagaac tgctttaagg aggtggaatg atgaactgga aagaactttg
     9301 gatgaccctt tttggaacta ccgaatggct tggtcttaat atcggctttt gggtggctat
     9361 ggcagttgtg ttgctgattg ttattgtaat gaatgtagta ttttggtcca tgaaaccaaa
     9421 aaaagacaca aaagagtctt ataaataaga tatcagtatg aatgaatcaa gggagatgca
     9481 cagtttttta ttgtggaatc tccctatcat atcaaggcgc attcaattgt ggcaatttag
     9541 tagtaaagta tgtgtttttt tatcattgaa ttacctataa atgctgtata taagtggata
     9601 attgtaattt cgatataaaa attatggtac ttaaaagaaa tttcgttttt tattgtttgt
     9661 ttacgcgtct ataacgaaat cgtgaaataa tattcaaagg tagattttat tatggaatgt
     9721 atgtctgtag aagaagcttc gataaaatga ggagatatct gaaagaagga taaaaaaatt
     9781 atgtaatgga aacagaatcc caaatctatt gtaacaacaa tgacaggtaa tgaatggtta
     9841 cgtagattga taattcctat tcaaattatt atctatctta tattgagtgg tgttttgtaa
     9901 ttacattttt atacaaaaag aatatgttga acgcacaatg ataaatattt tgtattcccc
     9961 aactaataga ataaagtttt tgcttggaaa gtatgttata taggcattat ggtttgtaat
    10021 gatcacgttg acagattata ccgctatata tgaagtcaaa gaacttaaat catttacaag
    10081 tgagataatc tatctaagtc taaaaaactg gatttctcaa tctattgtca atgagtccat
    10141 tacttattat ttgtgttttg caaagaaatg ttttctactc tagtttactt tttagttact
    10201 aaatatcggg aattggtttt gttggactat attgatctga aagtatacaa aatattattc
    10261 cgtggtctgc tgtaacccct ataagtttat tagatgcaca aggattgcta ccttatattt
    10321 caattctact ttgttatttt gcaagactga ttataaatac atactgcttt agagggttta
    10381 gtttagttct ttttgaactt ttgaataaga cgacggttat atagccgccg ttttattttt
    10441 gcctaacgtt ggacaaatta ctggatggtc ttgttttatc ggctctaggg gtagccgtat
    10501 gaaacactta catattcaac aactttcgac catcggtagt tcattaaaaa aacatttaaa
    10561 ttctacggaa cataatgctc ctatatgtgg ttttttacag catataacag gaataatttg
    10621 tttagcacaa gatcagtacg tctgtattgc ccttgtgctt ttttactgca taaaactttt
    10681 tcaaaaaatt tttgcggttg ggtatcaaat cccacctttc atggtgagtg ttagtgcgaa
    10741 aagaggtaga agtcttttcg ctttagcaac tttcgctgga aagggggtga gattatgaaa
    10801 ccatcttctt tcgagggcgc tatcagactt caatttgatt gtctagctcg caaggttgta
    10861 ggacgaactg taaagaacta tgaccgagag cttggcagac gtgcaagacg tgaaacgccg
    10921 ttctgtgatt tatctgaaat ggagttaaac cagatcggtg ccatggacga atattgcgtt
    10981 gagtttacgt catttgatgt atttggttca gaagtccata tctatgatga acgcttatgt
    11041 gaagctatca aagaattaac agaaagccga cgtaacattc tgcttatgtc ctactttctt
    11101 gaaatgaccg acgctgaaat tgcagaggtt atggaaatgg aacgattttc cgtatgtaga
    11161 aacaggctgc acacattaaa gcttattaag gatatgtacg aggaggaata aaaacatgat
    11221 gaagtctaca aaaaagtgtc catcattctc gaccatcagt tctgcagctg acggtaacga
    11281 ggtggcaatt gaaaagattt tgaaccatta tgacgcttac atatcaaaag ccagcctacg
    11341 cccgttctat gacgaacacg ggaatatgta tatcgtgctt gatatggaac tgaaaggcag
    11401 gatacgggca gcgttgatta aagtaatctt agggtttgaa gtcaaagtga agtaaatata
    11461 acagagagta tctaccacct cattttaact cttttccgtt ctttgacaag tgaatacagt
    11521 aatcagatac gtttgatata cgacgagcca gcggactgga acgccacgac cactgaaatg
    11581 gggaaagcag cgagcgatta tgccagtgaa ccgcaagcga tctttggaaa ggttgctgcc
    11641 atgactcgta tatctgaata atgatacacc cgtatgacac ggttcaccca tcagaatggg
    11701 aatggtgaaa gtccagtgga gctgccgaag ccgtctgatt acttccataa ataaaaaagc
    11761 aaggagggtt ttaatgaata atataattag gtatagtatg caaattgcta tgttaaaaca
    11821 actgcttgcc cgtaaactga tttctgaaaa agaatacact cttgtaaaac aaaggctgat
    11881 gaaagactat aaggtgcttt ctgacataac tgcttgaatt gtgtttttta aagtagtaca
    11941 ataacaatat gaaaggaaca caaaataagg aggtgtacta tgcaggaagt tgaagtaata
    12001 aaagcaaaaa atgttttggc aagacaagcc cgtggaaaag ccattgagcg tttgcgtgta
    12061 gctgcatatt gccgtgtaag tacagatagc gaagatcagt taaacagtta taaatcacaa
    12121 gttcagtatt atactgacat gattaagaaa aacaaggaat gggtgttagc tgacatttat
    12181 gctgatgaag caattacggg gacacaagta acaaaacgag aagattttca gcgtatgata
    12241 aatgactgta tgaatggtga aattgacatg gtatttacaa aatccatatc aagatttgcc
    12301 agaaatactt tagacacatt gaaatatgtt cgtatgctaa aagagagaaa tatcgccgtt
    12361 tattttgaag atgaaaaaat caatacactt acaatggatg gagagttatt gcttgttgtt
    12421 cttagttctg ttgcacagca ggaagtcgag aacatttcag ccaatgtaaa aaaagggttg
    12481 aaaatgaaaa tgaagcgcgg cgagctggta ggttttcaag gttgtattgg atatgattat
    12541 cacccagagg ataagacaat aactgtaaat caagaagaag cagagattgt cagatatatt
    12601 ttcaaccgtt atgttgatgg tgctggtggt tctgttattg gacaggagct tgaaagtcta
    12661 ggttataaaa cgaagtatgg gagttccaca tgggcaccat ctacggttat tggaattatt
    12721 aagaatgaga aatacaaggg tgatatactc ttaggcaaaa cttttactgt tgatccgatt
    12781 tccaaacgtc gccttgaaaa catgggtgaa gaagataaat tttatatgaa agatcaccat
    12841 gagccgatta ttagtgaaga agtttttgaa aaagcacagg aaattcttaa tcggagaaat
    12901 aagaaccgaa caacggtagg cagcggtaag cgtgagaagt atagcaggaa atacgcattt
    12961 agctgtatgc tagaatgtgg cttttgtggt ggtactttaa caagacgtaa ttggcacagt
    13021 ggctcaaaat acagcaaggt tatatggcaa tgtgtaacgg ctacgaagaa aggaaagaaa
    13081 ttttgtaaac atagcaaagg tattccagaa acagctattg aagaagcatt tgtagaaagt
    13141 tatcgtttat tatgtgatga taataaagat gttctggaag aatttttgca gagaatggac
    13201 gatactctta gtagtagtgt ggtgtcaaaa caacttgcaa aagcggaaaa ggagatagac
    13261 gcattagaga agaaaaaaag taaacttgtg gatatgcgtc tggaagaaat tattgataag
    13321 gaaacctacg aaagtaaata tgctgattta gtaagtaaac aagaacagct tgttgaagaa
    13381 agacaaaagt tacaagaaac ttctgataat gagaaagaca taaaaaaacg tttgaaagag
    13441 tttaagaaaa ctctggaaca gaatgaggtt cttgataaat ttgaccgcta tgtgtttgaa
    13501 agcattgttg aaaaagtaat tgtaggtgga cttgatgaaa acggaaatgt tgaccctgca
    13561 cagttgacct ttgtttataa aacaggcttg aagaatagcg tagatggtgc taaatttaaa
    13621 acacaaagga aaaatgcaag aggacggcat aggactaatg aattgtgttc acatgacagc
    13681 aacgaggttg ataaaatgtg ttccgatagt agtaacgaca catgtggaaa ctgtagctaa
    13741 gataatcaaa caatagtaat gtttatatag attatatact aataatatat tgtatttgcc
    13801 accgatttgc cactgtaaag cgatttcggt ggcaaattta gtctacaatg ttatcaaaaa
    13861 tctctaaagt tttattttta attctataaa acttattata attgtaattt taaaagaaat
    13921 agtcaatcta cagtagccag attttataca actcattttt ttcatagagt aatttacata
    13981 atctatagtt gaatattatt taatattttt ataattcatt agcaaatata ggcattacaa
    14041 atgttgataa acatgtgaaa gcacatatcc acttataatt atttttttta aaaccataaa
    14101 atcacctctt catttgttgt atgtttttat aacaaatgaa gaggtgattt ccagacaatt
    14161 taaaaggttt ttgtatattg actaaaattt atttttatta tttataattc ttaataatac
    14221 atatgtacct actgattcca tattaaactg tataattttt gttcatcttt ttttataatt
    14281 aaaactacgt agttctcata atttctctta tatatatact tatcatcttt tttaacttct
    14341 aacttatatt ttttaaaatc ttcttcaatt ttatttttat tacttttatt aatgttaaca
    14401 gaatctcttt taaatttatc aatatatttt tctatttcat taatattatc tttgcttaca
    14461 actataccac cttttaaatt aaaaactttt ttgaaatttt tttcagaaaa tccttgaata
    14521 aaataacttt gtccatctcc attaatacta tcttgagtat caaatacttc aatttctttg
    14581 ttaggtattg gtatatcaaa attccaagat tcctttagca ttgaaatatt agctgttttt
    14641 cctaaaaaaa gattatatag gatacttcct aataacaaaa cacctattaa tagtagtgtg
    14701 attaatacaa tttttctctt tttcattttt cgttcacaac cttttgaaaa aacatgattt
    14761 aattcatcat ttgaaggcat gtcttcaaga ataatttttg ttgtgatagg aatgctttct
    14821 tgaaacatat catttgtaat atcaaaatct tttctttgat tactcaatat ccatttcttc
    14881 taatatttct tttgattttt tctagtacat tgaatactct tatatacatt ctcctctttt
    14941 aaaattaaaa gttataaaag aataagttat ataaaaaatt attatatttt ttaatatagc
    15001 ttatttgcaa atttataatt ttgttaatct aatccttcaa actaaaaaat catttatatt
    15061 tacagaattt cattttaaga gatgtttatt ttcacataga taagagaatt taagttctca
    15121 tatttattat ttgatacaaa tttacatctt tattatatgt attttaaata aaaaataaaa
    15181 ttatgaatac tagaaatcta tcttaattag ctttctaaca taaggctatt taaagtagat
    15241 aaaactaaaa agggttctct agaaaaccaa atctatttaa ttatacttac ttactaatac
    15301 catcacttga tgcaaacaag attctaccac attttctccg tactctagta taaaatcatc
    15361 tagttgtgta tctataaaat cacaataatc atatgaacta ccaatataag cagtcaacat
    15421 atgtgtacat ctatccattt tatctctaaa catatatatt attatccccc taaaaaactt
    15481 tcataaaaac aaaaagaata tctctttttg gcaactggct gacataactc atagtttatt
    15541 ttataagcgt tagtccctat agctttgcgt cactaaattt ctctagtttt gccgatttag
    15601 ttttattcta caattacaat aatacaatag aattgcttat tattcaatat acttatttga
    15661 aaattaaata aatttacaaa tatgaacgtt attttatatc aaaaaaaata gactatgcat
    15721 gtagaatata tggaaaaact tgattgtagg ttttgtatga aaagacagtt gttattaatt
    15781 taagtgagat aaattgagca acaggcataa aatgagaaaa caaataatag cagttaattt
    15841 ttattattaa tactaggtga acattaaatt tcaaaatacc actagatttt atagaataag
    15901 aaaatgagac aaacataaat atatgaatat attaaattat acttgaacat acttatattt
    15961 tatgataaaa tttcaataat aattattagt gaaggagtgt cttgcctgat gtcagagtta
    16021 tatataatgg taaccaacaa ataaatagtt ggagacgcat atgggttctg tttaatcaga
    16081 gtttttaagt gtatttttga tacatttgat tgttgtgtct tactctgcct ttatagataa
    16141 ctaacattag acaacaaata ataatattac atattacttt gttaaattgt ggtgtagtgg
    16201 gctttaatct gctacacttt tattaatttt acacagtatt attttgaata taattatgtt
    16261 gaaatagaag atactaaatt ttctagtcca caggggtttt ctgtttttgt gtaggtaaat
    16321 atgtatacca cataaaaagg cagatataaa tacctgtgga ctttttttgt agaaaataca
    16381 cgatacggag gaaataaaaa tgaattataa aaatagaaaa cgagcttatt atcaggaaaa
    16441 ttcctgtatg gataacttta tttgcaaaaa ttgtggttgc cttgtagttc ctgaaggagc
    16501 tggtacacaa catagaaatc attgtccaca ttgtttacat agtcttcatg tagatatcat
    16561 tcctggtgac cgtgctgcag attgtgatgc taatatggaa cctattggag tgtgggtgag
    16621 aaaaaatggt gagtgggcaa ttattcatcg ttgtacacgc tgtggacatc ttagttctaa
    16681 tcgtattgca gctgatgata attcagtgaa attaatgtca attgcattaa aaccactggc
    16741 acagccccct ttcccactag aaagaatcat tgaaaagttg gaaaaggagg aaacagaatg
    16801 aatggtatga aacaaatcaa tttacaagac tatggtctaa atagaaattt tattactcaa
    16861 agtatggaat atcatgattt ataccttgct cggattatct cgcaggaaaa gaacttttat
    16921 caagctgtat gtgtaaatgg tgtactgttt gcagaggttt caggaaagct gcgttttgag
    16981 gctactatac aatctgattt tcccacagtt ggtgatttcg ttatgttgga tcgttgcaat
    17041 aataaaaatg gtaatgctat tattcatcat gtgcttcata gaaaaagctt gtttgtgcgt
    17101 aaggctgctg gaaaggtaaa tgaggagcag gtggttgcag ctaatattga cattgttttt
    17161 ttgtgcatgt cactgaataa ggattttaac attcatagat tggagaggta tctgtcttta
    17221 acatgggaaa gtggagctat cccagtagta gtacttacta aatcagactt atgtgacaat
    17281 gtgcatcgta aattacaagt agttaattct atagccttgg gtgtagatgt agttgttacc
    17341 tcatctatag agccagatgg ttataaacag atttctaagt atattaaacc aggctgtact
    17401 gttgccttac ttggttcttc aggagttgga aaatcttcat taattaattg tctgatgggt
    17461 ggagaatttt taaatacaaa ggaaattcgc atagatgatg ataaaggaag acatgccacg
    17521 actagacgag agctattttt aatttcaggt ggtggtattg ttattgacac accaggtatg
    17581 agagagttgg gaatgtggga tgtttcagaa ggtttagata aaagttttgc agatatagaa
    17641 gcttatttcg gaaaatgtca ttttcgtaat tgtactcaca cctcagaacc aggctgtgct
    17701 atctgggaag ctattgctca tcaagaactt tcgcctaaaa gatgggaatc ctatcgtagg
    17761 cttaaaactg aagcagtatt tgcagacaat aaaagcgaat acttagcaaa aaaagaaaag
    17821 aaatttaaaa atatctcaaa aataaataag aagattagaa gataagtgta gtttttttgt
    17881 tcattctatg agtctatata ctattaatat attaaatttg ccatcaattt gttactttaa
    17941 tgtgattttg atggcaaatt tggtttgtgg tattatttaa agcaatttat taattttttt
    18001 aataaagttt gattttttat atgatgatat atacatgtga taatgtatac atatttgtta
    18061 cgataggctc ctttataaag aggtcttatg taagataaga aaaggcatat ttatactaat
    18121 acataataga gtatatgctt atgtatgata gagatgtttc ttaagagcaa aactgcaata
    18181 ttgctaattt ataaagtaaa aatatatgaa ttataaaata caaataatat atttataaaa
    18241 attattgttt taatttataa taaaaatttt ttgttgaaaa taatgctcga acctataaaa
    18301 ctattaacta atatttgtaa atgtatactt ttgatgataa aatataatta agttaagttg
    18361 gtttttataa acataaattt attttaagga ggtgaaataa atgaaaatat ctagtcaata
    18421 tagaagtcaa tattcattta gatatgaaag taatataaat aatacaagaa taaatgaaag
    18481 tatggttaag aaaaatgaaa ctgtaggaaa agacacttat ttatctaata ttatgaaaca
    18541 aaagcaagaa cttaatgata gaattagaga tttaaaatat agacaagagg tttatactaa
    18601 aaaaataaat gacgcaatta agaacttatg taaatcagaa ataagagaaa caactaataa
    18661 tttttctaat atagaaatag gtattaaaaa tagcattata gaagagaaaa ataaaagtac
    18721 aatgttagat gaaaattcaa cttatctaaa tacaaatgat gaaaaagaat ctttaatcac
    18781 taaagagtct aatgaaaaaa ttgaagaaga aatattaaat gatgaaaaat tagaagagtt
    18841 agaacaaaaa aaggattata aagaggattc taataaaaaa gagaaagtat ctgaggactt
    18901 atctttagta ggtaaaactc gtgaagagct tgaaaatatg cttaaaaatt ttataaattt
    18961 aacacaagaa gaaataatga aacttgagtc gagaatagaa aagttagata aaaatgctga
    19021 agaatacaaa caaaattcaa agactaatat atttgataaa acagatgaac aaaaaaaaca
    19081 tataaatgta ctgatttaaa caatatgttt taaaaaaata agtttattat ataatgaaaa
    19141 tataaaggta caatttcaat acacttctat taaaccaata atttatgaat cagatttaat
    19201 ttcaatttac taataaccat atatttatga gttaataatt ttatgtttta aaaagaatta
    19261 aataaggggg agtagtatga agaagaatat attaactact atgattacca tgattattat
    19321 attgatgatg agtatgacag taatggcaga gccatatcaa gaagatgaaa catctagctt
    19381 gaaaaaacag agtgcaaatg aacgtaatca gactaatata ggcacaatgg atggcagagt
    19441 gcaaacaaat gcaataagta aaggtgctaa ttatgaccct aaatatcctc ttaaaggaat
    19501 gttaacacaa cttggtctta atacaacagg taaagaggga tgggatttac tctatggagg
    19561 aaatcccaat ggagttgagc ttcgagcaga tggtaaatat tattatataa ataagggtat
    19621 ggaagggcta ggattttatg cggcagcatt gtcaggtggc tcatatcaga gaataggctt
    19681 tggaaaactt gaccctatag aagtaatagc tgatggaaat tggatttctt acaaacaagc
    19741 tcaagataca cttacagtaa tcaggaattt tttaaatagc tttgactgga gaaatgcatc
    19801 tgagatggaa agagctaata gagctgcaaa attggtaacc gaagcaaaat atgcagactc
    19861 taaatattgt aacatagtct atggtaatct ggttgataaa cgtggtgtat gtggaagttt
    19921 tgcaagtagc tttcatttat taacaaggtt aatgggaatg gattcactat ctatattaaa
    19981 tccatcatta aatcatgctt ggaactatat acaaattgat gggaaatggt ataggtttga
    20041 tgggtctgaa atatcagctt ttggtggatc attagacttt gattaccgta aattaaagga
    20101 tgcaacaaga gagatgacta cttactatga tgctaaggca ttgtctatat taggatttaa
    20161 tcaataaaaa tagggggagg cttcttatag agcctttttt tattgatttg aaatttaata
    20221 aggaatttta tatgaaaatt taatcaataa aagcaaaaaa ggctctttat aaagcctttc
    20281 taagtgtttt tttaagtaaa attttttata tcaacatcag ttttaatcaa aacatataac
    20341 attttggact tacttttaaa aataaaatta ttaaaatata atattaatca tgtttttagt
    20401 ttattatatt ctataatagt ggtcatgttg actattgtag ttttattaag aatagttggt
    20461 ttatataaat atatgcaaag atagcttgtt gtttacttta cttattagta tataggacaa
    20521 gatactctaa taaatttgcc tattttaata tcttttgtac taaaaccaac taaagtttcc
    20581 tttattattt cgttttgaat aatacaaagc ataatccgat tgtttgtata aagactggaa
    20641 agtatcagag ttattttcta atttagatat acctatagaa caagaattac tctctaggca
    20701 taattctaaa accttctttg ccttattttc aacattctct tttgattgat tactatattc
    20761 aaaaacagca aattcatctc caccaagtct tccaataata gcatcattta cgaatacttt
    20821 agtcaaaata tttgaaaaat gtattagtat ttcatctcct cgaacatgac caaagatatc
    20881 atttacctgt ttaaaattat ctaaatcaaa gatgtaaaag tatccatgtg aaacagtttt
    20941 aagcatttct tttatgtgta cttcaaaaaa atgtctattg tatagtccag tcataaggtc
    21001 tttttctgat tttagtttaa attcatttgc cattgctagt gcttcttgtg ctttttcagt
    21061 tattgtttta gggtaatact catctttcat ttggtcaaag tttggaatag aatggtgaac
    21121 atggataagt ttaatcatat catctgtaac tctgtatacc atagtaaatc gagtatccat
    21181 ttctacatag atatcttttt ctaggtaatc tttttctatt acccaaagag taccatatac
    21241 tataaaagct tcgtctgtaa ctttggtaca tgaataccac tcatctataa tctcaaagtc
    21301 tatttgctct gattctgttt ggtctttttc aagacctata ataaattctt ctaatgtttc
    21361 gtaaaattca tgttttcctg ttccaattaa tgcaaggttg tcatctaata aggaaataat
    21421 aaatttaagg ttttcttctt ctggatttaa aatataaata tgccaaattt tttttgataa
    21481 atctcttaat tgtttttgta ttgtattatt ttccatagat taccccctta agatttacag
    21541 tttatatttt aatgtattat aagttgaact cttattacaa tattattata atatagaact
    21601 aaagaaatta catcctgtat aagtaaaagt atatctttta taataaaaat attacagtaa
    21661 aataaccagt tttaatatac gtattatatt tttaatttat aaacgaatat tataaatgaa
    21721 atttaaattc aaccctgaag aaataagcta aatgtattag tatataaatc catacttgaa
    21781 cataaataac ctttatgata tgatatcaat aataatatta tgagaggagt gccttgatgt
    21841 cggagttata taatggtaac caacaattaa atagttggag acacatatat tctgtataat
    21901 cagaaatttc aagtgtattt ttaatacatt taatttttgt gtcttattct gcctttatag
    21961 ataactgaca tatgacaaca aatagtattt aaaatatata ttactttgtt aaattatggt
    22021 gtagtaggct ttaatctgct acacttttat taattttgta tagtattatt ttgataacta
    22081 ttttgaaata gagagcatta atttttctag tccacaggag ttttctgttt ttgtgctgtt
    22141 aaatataagc acagagaaag gcagatatat acctgtggac tttttttata taaattatga
    22201 aatatggagg tagttaaaat gagtataatt gaacttaatg aactgactaa atcttataga
    22261 agatttgaga aaaaggcagg atttatgggg agtgtaaagt ctttatttaa taggaaatat
    22321 acagaaaaaa cagctatatc gtcatttgat ttaaagatag aagaaggaga atttgttgga
    22381 ttaataggtc aaaatggagc tggcaaaact actcttatta agatgttaac tggtattatt
    22441 gcaccgtctt ctggagaaat atctgtaatg ggttattacc ctaacaaatt agaaaataat
    22501 tttaaaaaac aatatgctgt tgttatgggc caaaaaagtc aactattctt tgaattgaca
    22561 ccagcagata cgtttctttt atttaaagaa ttatatgata tatctaatga agaatataag
    22621 aaaaacttgg attactttat cgaattattt gaggtatctg actatttaaa tgttcaagtt
    22681 aggacgcttt ctttaggaga aagaatgaaa atggaactaa tagtagccct tctacataat
    22741 ccaaaaatat tatttttgga tgaaccaact ataggcttag atgctatagc ccaaaaacaa
    22801 attaggtatt tcctaaaaga agtaaatgaa acaaaaggaa ctactattat acttacttct
    22861 cattacatgg aagatattaa gagtctctgt agtagaagta ttgttgtaga aaatagccgt
    22921 aaaatatatg atggttctac agagaaacta tttaattatt ttcaaaaata taaaaaaata
    22981 acagttagtt tttccaatga aactaacatt gtatttccaa aagattgtaa agttgttgaa
    23041 caaacaaatc ataagatatc agtaattgtg ccaaaagaaa gaggaaatga aacgattaaa
    23101 tggatttttg ataaatatga tgttaaagat gtaatgattg aagaagaaga tataggtagt
    23161 attgtagaga ggatatataa gaaaggaagt gatgagtatt atgagcaact ataatgaagc
    23221 aagttcaaat gattcaatgt tttataaatt taaacagagt attagaaggt attatgaagt
    23281 agccaaaatt aactttaaag tgcaacttat ctggagattt gatgtggtgg ttaatatggt
    23341 gtttacgatt gtaaaaattt tatttgctta tattttatgg aaagcaattt ttggtgaaaa
    23401 taaaatagtc tctggtttta catttaactc tatgttatct tactatataa taagctcatt
    23461 tttagcaggt attgatatgt caaagagtac cagtgaagag atatgtaatc gtgtaaaatt
    23521 gggcaccttt tcaaagtaca tgattattcc aattaatgtt caaaattatt ttattgctaa
    23581 aaattttgga atgacatgtt tttattttgt atttaatatg atttctgcat ttgtatggat
    23641 ttttatattt aaaatagatt ttgcctttac atcaaatcca gttcaaatat taatttcaat
    23701 tttgatgatt tttctagggt ttatttttat gatgaaactc cacttcttta ttgggatttt
    23761 gtcatttaaa tttcttgaga ttagtttttt aaaaatgata attgaaaata ttgttgaatt
    23821 tattacagga acaatgatac cacttgtact cttaccagaa actatagtaa cagttatgaa
    23881 ggtatttcca ttttattata caacatattt gccatctatg ttactaatag gtagaaacag
    23941 caatgaggca atacaaggat taattattct agcattatgg gttttatttt tttctgtagt
    24001 aaataaagtt atgtataaaa gaatgagaac attatttgat ggggtgggta tatgatgaaa
    24061 aaaaatttga aaataatatt tatgttatta aaaatgaaat tatcaaagat gatggtattt
    24121 agattcgatt tttttggagc attttttgta gatagttcat tatttatatt acaacttcta
    24181 atgtttaata gtatatattc tcatgtagac tcaatagggg gatggcaaca aggagagatg
    24241 ttaatattta taggaacttt ctcattaata aatgcgataa atatgacgat tttctttttt
    24301 ggtatttatg atattccacg aaaaatccaa gaaggagaat tagactacta tattacaaaa
    24361 ccattagacc cactatttag gttaacattt gaaaatatta atattggttc atcaccatta
    24421 attctagcaa gtatacttat tgttttatat ggaatatctg agttaaactc agagataact
    24481 ttagttatga taataatata tattttcatg gtgttattga tgacattact tttttatgat
    24541 gtatgtatca tacttagaac agtttcattt tttgttatat catcttctgc gataatgaga
    24601 ttagaagata atttattgcc aatgaatatg aaaattcctg gtgtaatata tgaaggtatt
    24661 tttaaagtat tattctattt aatcttacca tatggaatta tgtcgacaat tccgactcaa
    24721 attttggctg ggacaataac aattaatgga ttaatatact caattttaat tgtatttttg
    24781 tttacattgt ttatgtggag attttggaag ttaggtatga gacattataa aagtgcaagt
    24841 agttaataaa ttagtaaaaa tagacaatgt ataactatat ataaatacgt ttcatatgac
    24901 actaaactat gtgaacaagt atttgcgcta aaggcactga aagctataaa atgattaaag
    24961 taaggattta aaagtttctt tagtagaaat ataattgtgt aaaaaagcaa gattaattaa
    25021 aactatatcc aaataattag atataattct tatgcagaaa ggaggtctgt aaatgtctaa
    25081 ttgtttggaa tatattttaa aaataattaa tgtacagaaa aataaacaaa atgagtgatg
    25141 tatattattc agaaaatcat gaacaagata atctttatga gcaagataat ttactggcac
    25201 aaagatttaa tttatttact ctattgaagt ttgcatttcc tactattttt atgatgattt
    25261 ttatggggtt gtatacaatc gtagatacta tatttgtatc aagatttatt aatacaaatg
    25321 ctctatcagc aattaatatt gtatgtccaa ttattaatct tatagttgga attggaacta
    25381 tgcttgccac tggaggaagt gctattgtag cacgtaagat gggaaatggt gatattgagg
    25441 gagcaaataa agctttttct ttaatcgtat tatcaggagt tgtaattggg gtaataatta
    25501 ctggtatagg tattgtattt atggattata tcattatatg gttaggagca agtgaagttt
    25561 taattaaata ttgtcgttca tatcttttta ttatcttggt ttttgcttct gcaaatatac
    25621 tacaagtatt atttcaatgt ttatttgtaa cagctggaaa gcctcaattt ggattatggc
    25681 tagtgttagg tgctgggtgt gctaatataa ttcttgacta tgtgttcatt gttctgttac
    25741 aaatgggtat tagtggagca gcacttgcaa ctggagtagg atacctaatt cctaccattt
    25801 caggtatggt tttcttttca aaaaataaag ggttacttaa attttgtata cctagactag
    25861 ataaaagaat attatttgaa gcttgtttta atggttcatc tgaaatggtg ggtcaactat
    25921 caacagcagt tactactttt ttatttaatg ctatgatgat gaaattaatt ggtgaagatg
    25981 gggttgcagc tattacaata attatttatt ctcaatttct tttaacttcg ctttatatag
    26041 gtttctcaat gggtgttgca ccaataataa gttataacta tggaagtgaa aataatacgc
    26101 agttaaatca tgtttttaag atttgtatat tatttatttc agtagcatct ttattggtat
    26161 ttatcttttc tatatcatgt tcaccaatca ttgtagagat tttttctcaa agaggaagcc
    26221 gagtttatga aattgcaagg tccggatttt atatattttc ttttagtttt attttttgtg
    26281 gatataatat ttttacctca tctatgttta cagcattatc aaatggaaag gtgtctgcaa
    26341 tattatcttt tctacgtaca tttggattta taactatagg tttattgata ttgccaaata
    26401 tattaaatat aaatgggata tggttagcag taccaagtgc tgagatttta accttatttt
    26461 taactgtttt ttgtatattt aagtataaga atagatataa atatatgtag atattaaata
    26521 aatagcttag atagatattt tatgttttaa taagtaattc aattagtggt agatatatac
    26581 tattgaaaat taaagttata attgatataa agaattaata aaaataacat ataaaagttt
    26641 aaaagggagt tgaacttaaa gtttgattag aaaaagtcaa ttttaagaca actcctttta
    26701 ttttactatt gtattaatct gtatctgtaa gtgtattagt ctaatattat aaactttgga
    26761 tgaaattaat tttctatagg ttttaatgta tcatacactt tattttaaag aaacttatct
    26821 taattttcat agtgattgaa tttgtctatt agagatgtta ctattagtaa tgtaaaagca
    26881 agagtagtga atataaaaaa caagatagtg atactgcaag attagaatat aataaatttt
    26941 tatggagaga tttcaatatg gatacatgtt ttaataaaaa aatattactt gttgatgatg
    27001 aaaaggatat agtagattta attgaagaag tactcataaa tgatggtttt aaaaacatta
    27061 ttaaagctta taatggtcta gatgctattt cgctgtgtaa gatagcatgt ccagatgttg
    27121 tcattcttga tataatgtta ccagatatag atggtataga agtgtgtaaa aaaattcgag
    27181 agttttcata ctgttctatt ctttttttat catcaaaaaa tgatgatatt gacaaaatac
    27241 taggtcttag tagtggggga gatgactata taacaaagcc attcagtcca agagaaattg
    27301 cttttcgagt taaagcgcaa ttacgtagac aacagtatca aagcattgta ccatcagata
    27361 gtgaagttat taaaatagga gatattacta ttgatataga aggaaaccgt gtatataaag
    27421 atagaaatga gattgagtta actggaaggg agtatcatct tttaagctac atggctcaaa
    27481 atgtaaataa aattatagga aaagaaagac tctatgaaca agtatgggga gtatatagta
    27541 gtatttgtga caatacaatt atggtgcata tacgacatat aagagaaaaa attgaagata
    27601 acccctcaaa tcctaaaata cttataacag ttaaaggact aggatataaa cttgtgaata
    27661 gaatcgatta gaatagattt taatattaat gaagatatct caagttgaat ttttagttaa
    27721 tgaggcattc ttttatgggt gaaatcaaat gtattttcgt tatctcaaca atgaagaaga
    27781 ggtctatctc tattttgaga tagcctcatt aagtttatat cctggtaatg tactttatgt
    27841 tctaataaaa tttttttagt ttatttgcag tgaaacatta actagaacat taaaatttgc
    27901 attgaaatac caattaaata atattgtata ttgtgtataa acttatacta attgtttttc
    27961 tatattaaat actttatcaa tccaaccttt ataaaatttt tcttcatggg aaactaggat
    28021 tatacttcct ctaaactgtt tcaaagcatt ttgtaaagca tcctttgtct ctgcatcaaa
    28081 gtgattggtt ggttcatcca gaataagaaa attacaagga gataacatca tacaacataa
    28141 tttaacctta gattgttcac caccacttaa agtagataca ctccttgaaa catgctcttc
    28201 cttcacacca catctggcta gatgacgtct aatctctttt gtatttaatt ttggatattt
    28261 atctgctact atttgaagtg gggttttatc aggattttcc cattttaaat cttgctcata
    28321 ataaccaatt ttaacttgtt ctgaaaactg gaaatctcca gatatacgag gaatatcttt
    28381 gactaatgtc tttaacaagg tagatttacc gattccatta aaaccagtta taactatttt
    28441 ttgacctcca tcaacagaga aatttaattt tggaagtaaa gaatagtagt atccaacttc
    28501 aagatttgtt atagtaagag cattttgagc cgatatttca atttctgaaa attgaatatt
    28561 tggttttcct gtaaaactag gaggagctat tctttctatt ttatccaatt gtttttgtct
    28621 tcctcttgca atttttgaat ttactccagc tttattttta cgtataaatg tttcttcttt
    28681 ttctattttc ttttgttgtg cttgatacct tctaatataa tcttctctta aatgtgattt
    28741 ttgtttaagg aactctgaat attttccatg atatttctta atcatgccaa attctatatc
    28801 aagaatacct gtagaaattt tttctaaaaa gtcaaaatcg tgggaaacta caataaatgc
    28861 cccattaaat gtatttaaat aatttgagag ccattcaaca tgctctttat ctaaaaagtt
    28921 tgttggttcg tctaataata gaacatcatg gttagaaagt agtaattttg ctaaaattac
    28981 ctttgccctt tgaccaccac taagctctct aacaacacga ttcattccta ttgaatctaa
    29041 tccaagacca tttgctattt tatttatttc attatctata gaatagaaat tacttgctat
    29101 tagttgttcc tgataatctg atgctttgat tagatattgc tcattttcag atatagcact
    29161 tttttgatag agtaaattca tttccttttc tattttataa agttcttcaa atgcagtgtg
    29221 taaatagagt gatattgttg tatctctatt gatttctgca tattggtcta aatggcctat
    29281 attgatatta ggttgccact tgatttctcc tgaatctgga acaacttctc ccaataaaat
    29341 tttaattaat gtacttttac ctgtaccatt ttggccaact acacctatat gttcaccttt
    29401 gaataaatca aagttagctt tctcatacag tgatttatcc ataaaacatt gtgataaatt
    29461 agttactttt aataaactca taattcaaac tccttttctt ttattaaaaa atttatggaa
    29521 ataaaaaaag cagaggaatg gcattgtaaa atgccgacct ctgcttttgg aaaaacaaaa
    29581 ggtcatagac aagcattgtt gtctatgacc agaaaatcta tattatctat cggcaaatag
    29641 gtaattttaa ataagcatag ccaaaaaact atactaaacc tatatttgcc gatgaagata
    29701 gaaagcttta agctttcaga ttaacctcta cacaacgctt catcgtatac atttgtgtag
    29761 agttaagttt tctaacaagt ttaactaatt ctatataaat cataattatt cacatcctaa
    29821 tcttagtatt aattctagtt tatctgtatt taaattgtac ttttgtactt tgtggattat
    29881 cttatttatt taaaatatgt tttattaatt atagcttaag ttatgttatc ataatactta
    29941 gttatagtca ataagaaagt tatatttact attattatat taatttagta aatctatcat
    30001 tacaaagtta tttgaagact cgctatcaat aaaattttat agtctataag aaaattaaga
    30061 ttatgagttt tataattttg atatagggat actattttca taaatattac aacatgtaga
    30121 agtatatgtc tattattgtt gaattttcaa tgatatatta aggttctaat atattataca
    30181 aaataaaaac attaattcaa cttaaataat tttaagtatt atatgtgttt tgattaaaga
    30241 a
//
LOCUS       DBGJFU010000040        29559 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.040, whole genome shotgun sequence.
ACCESSION   DBGJFU010000040 DBGJFU010000000
VERSION     DBGJFU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.040"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            105..1019
                     /gene="murB"
                     /locus_tag="ACOIAS_001712"
     CDS             105..1019
                     /gene="murB"
                     /locus_tag="ACOIAS_001712"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGZ2701425.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            1023..1736
                     /locus_tag="ACOIAS_001713"
     CDS             1023..1736
                     /locus_tag="ACOIAS_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HGZ2701426.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            1767..2624
                     /gene="rapZ"
                     /locus_tag="ACOIAS_001714"
     CDS             1767..2624
                     /gene="rapZ"
                     /locus_tag="ACOIAS_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGZ2701427.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            2636..3742
                     /gene="yvcK"
                     /locus_tag="ACOIAS_001715"
     CDS             2636..3742
                     /gene="yvcK"
                     /locus_tag="ACOIAS_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGZ2701428.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            3811..4236
                     /locus_tag="ACOIAS_001716"
     CDS             3811..4236
                     /locus_tag="ACOIAS_001716"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGZ2701429.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            4253..5200
                     /gene="whiA"
                     /locus_tag="ACOIAS_001717"
     CDS             4253..5200
                     /gene="whiA"
                     /locus_tag="ACOIAS_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HGZ2701430.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            5357..8926
                     /locus_tag="ACOIAS_001718"
     CDS             5357..8926
                     /locus_tag="ACOIAS_001718"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGZ2701431.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            9135..10094
                     /gene="pfkA"
                     /locus_tag="ACOIAS_001719"
     CDS             9135..10094
                     /gene="pfkA"
                     /locus_tag="ACOIAS_001719"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGZ2701432.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            10146..11906
                     /gene="pyk"
                     /locus_tag="ACOIAS_001720"
     CDS             10146..11906
                     /gene="pyk"
                     /locus_tag="ACOIAS_001720"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGZ2701433.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            12081..13445
                     /gene="rlmD"
                     /locus_tag="ACOIAS_001721"
     CDS             12081..13445
                     /gene="rlmD"
                     /locus_tag="ACOIAS_001721"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGZ2701434.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            13688..16732
                     /locus_tag="ACOIAS_001722"
     CDS             13688..16732
                     /locus_tag="ACOIAS_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2701435.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAILPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHK
                     HNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKAEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEKPEVPEKLETPQTPEKPN
                     RPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKKS"
     gene            16858..17184
                     /locus_tag="ACOIAS_001723"
     CDS             16858..17184
                     /locus_tag="ACOIAS_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2701436.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            17200..17583
                     /locus_tag="ACOIAS_001724"
     CDS             17200..17583
                     /locus_tag="ACOIAS_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2701437.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            17685..18122
                     /locus_tag="ACOIAS_001725"
     CDS             17685..18122
                     /locus_tag="ACOIAS_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701438.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            18216..19610
                     /locus_tag="ACOIAS_001726"
     CDS             18216..19610
                     /locus_tag="ACOIAS_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HGZ2701439.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNLRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGFLAQARQ
                     DGTATCEAKGDGSD"
     gene            19795..20991
                     /gene="mobT"
                     /locus_tag="ACOIAS_001727"
     CDS             19795..20991
                     /gene="mobT"
                     /locus_tag="ACOIAS_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HGZ2701440.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            21004..21138
                     /locus_tag="ACOIAS_001728"
     CDS             21004..21138
                     /locus_tag="ACOIAS_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HGZ2701441.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSISKEADYEMKQLEESEDN
                     "
     gene            21139..21360
                     /locus_tag="ACOIAS_001729"
     CDS             21139..21360
                     /locus_tag="ACOIAS_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701442.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            21399..22193
                     /locus_tag="ACOIAS_001730"
     CDS             21399..22193
                     /locus_tag="ACOIAS_001730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701443.1"
                     /translation="MGTINYFLKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYE
                     KYLLELVNKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKE
                     LTGQIQKFCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFD
                     IQTYVKLLTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAE
                     YDTFLAYIYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            22292..22588
                     /locus_tag="ACOIAS_001731"
     CDS             22292..22588
                     /locus_tag="ACOIAS_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2701444.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     KDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            22507..23010
                     /locus_tag="ACOIAS_001732"
     CDS             22507..23010
                     /locus_tag="ACOIAS_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2701445.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            23028..23531
                     /locus_tag="ACOIAS_001733"
     CDS             23028..23531
                     /locus_tag="ACOIAS_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2701446.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            23649..24047
                     /locus_tag="ACOIAS_001734"
     CDS             23649..24047
                     /locus_tag="ACOIAS_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2701447.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            24025..26475
                     /locus_tag="ACOIAS_001735"
     CDS             24025..26475
                     /locus_tag="ACOIAS_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2701448.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYEMIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            26475..28679
                     /locus_tag="ACOIAS_001736"
     CDS             26475..28679
                     /locus_tag="ACOIAS_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HGZ2701449.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            28676..>29559
                     /locus_tag="ACOIAS_001737"
     CDS             28676..>29559
                     /locus_tag="ACOIAS_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HGZ2701450.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQTMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVT"
BASE COUNT        10566 a         4440 c         6447 g         8106 t
ORIGIN      
        1 tgataagcaa aatcatatat tattgaatat agttaatact atatactaat gtaatttaaa
       61 taataatgac agctaaagtt cagtataatt tgggaggaaa tacaatgaat aatcaagata
      121 tatatataaa tttattaaat atattaagta aagaggatat aaaagtcgat gaacctatga
      181 aaaaacatat ttcatttagg gtaggaggac cagcagatat attagttaga cctagaactg
      241 aagaacaatt aaaaaatgta cttaagttgg taaaagaaga atctattccg tatttaataa
      301 ttggaaatgg ttcaaacatt ttaattaaag atggtggaat aagaggagtg gtaatagaac
      361 tagctgataa tttcaattct tatgagataa atgatactag gatgactgct cagtcaggag
      421 ctttattatc agttttaggg aaagctctcc aaaagcaaga attaaaaggg tttgagtttg
      481 catcaggaat acctggcaca ttgggaggag cattagccat gaatgctgga gcatatggtg
      541 gtgaaatgaa ggacatagtg aagtcagtaa gactgatgga tatggaaggc aatatatttg
      601 aattatcaaa tgaacagatg gaatttgggt atagaaaaag tataatatcg aaaaacggat
      661 atatagcttt atcagcagaa ttggaattac aagaaggaaa ttatgatgaa ataaaaagtt
      721 taatggatga tttagcgacg agaagaataa ctaaacagcc attaaatttt gctagtgcag
      781 gaagtacatt taagagacca acaggatatt ttgcaggtaa attaatagaa gaaactggat
      841 tgagaggtct tactttaaga ggagcacaag tatctgagaa gcattgtgga tttgtagtca
      901 atcaaggaga agcaagtgct aaagatattt tggatttaat atatgttata aaaagtgctg
      961 tatatgccaa gtttggggtt atgttggaag aagaagtcaa aatacttgga gaggattaac
     1021 aaatgaaaat actggctgat tatcatacac atacattata tagtcatggc aaagggacta
     1081 ttgaggataa tgtaaaggta gccatttcta aaggaataaa aacaatagga atatctgacc
     1141 atagttataa acatattgca tatggtgtaa agaaaaaaga tattctcaaa atgagagaag
     1201 aagtagatta tttaaatgat aagtacactg atataaatat acttttaggt atggagtgta
     1261 acatattaga tgataaagga aatatagata tggatgataa atttattaga gattttgatt
     1321 atattatggc tggatatcat tttggttcta ctccaacatc attaagaagc ttattaaatc
     1381 attgtaataa ctatattttt aaaactgaaa aagcaaaaga atataataca aaagctgtaa
     1441 taaatgcgat aagtaataat gatattttta taattactca tccaggagat aaaggcgatg
     1501 tgtatataga agaagtagca aagacagcaa aaaagacaaa tactagactt gagataaata
     1561 gtagccattc ttttttgaat gtaaatcaat taaataaaat aaaagggcta gaaaataaat
     1621 ttataatagg ctcagatgca cattgtccag aaagagttgg tgattttgaa ctagctatga
     1681 agactatatc tgactctaat ctggatatat cgttagtaga aaacattata atataataaa
     1741 cataagataa aagggaggag ttagcaatga aatttgttat agttacaggt ctttcaggat
     1801 ctggaaaaag tgagacaatg agagctttag aggatatggg attttattgt gtagataatc
     1861 tacctcctgc tttgataact aaatttgcag aattatgtta tcaaccaaat tcaagtattg
     1921 ataaagttgc tctagggata gatataagag ggagaaagtt ttttgaagcg cttcatgaaa
     1981 gcttaaatta tttagaaaaa gaaaattatg aatatgaaat ggtatatttg gattgtaatg
     2041 atgatgtatt gttaaagaga tataaaatga caagaagaaa tcatccattg gcaaaagata
     2101 tgcagattcc agaaggtata aaaatggaaa gaaagataat ggaaccttta aaggagttat
     2161 ctacatgtat aattgataca actaacatga aacctaaaga ccttaaggaa gaaataaaga
     2221 aaatatattc ttcaggtgaa gataatccaa acctaactat ttctgtagta tcttttggat
     2281 ttaaacatgg tatacttgct gatgcagatt tagtatttga tgtaagattt cttccaaatc
     2341 cctattatgt tgaagagtta agagctaaaa ctggtgatga taaagaagtt agagattatg
     2401 ttatgaattc taaaatcagt gaagaatttt atgtgaaact tttagatatg attcattttt
     2461 tagtacctca atatatagag gaaggaaaac aacatctagt aattggtgtt ggatgcacag
     2521 gtggaagaca taggtcagtt actataacaa atcttatagc ggaggattta tctaataagg
     2581 gatatagagt tgtaaagaaa catagagact ctatgttgcg ataatggagg caaacatggc
     2641 taaattactt attattgtag gaattttagg ttggattgca ttaattgtat cgttatttat
     2701 atataaaaaa gcaaaaaatg aaaaagtaaa actatttcaa aggtctattg ttgaaaatag
     2761 agaaccaaat gtggttgtga taggtggagg tactggtcaa tcggtatttt taagaggtct
     2821 taaacacact acgcaaaata taactgcaat tgtaactgtt gctgatgatg gtggaggttc
     2881 aggtgtattg agagaagact taggaatgct cccaccagga gacataagaa actgtttatt
     2941 agcacttgca aatatagagc caacaatgaa tgaagttatg caatataggt ttacagaagg
     3001 gcttttaaaa ggtcagagtt ttggaaattt gtttttggct gctatgaatg ggttgtatgg
     3061 aaactttgaa aaagctgtat ataaattaag tgaaattttt gctataacag gaagagtttt
     3121 accagtaact ttggaagatg taaatttagt tgccaaatta gaaaatggca atataataaa
     3181 tggagaatct agtatacctg aagagtcaaa aattcaaaaa agctctattg ataaaatttt
     3241 tttaaatcca aaagatgtaa aaccattaaa ggatgttatt gcatctattt atgatgctga
     3301 cattataatt atgggtcctg gaagtttata tacaagtata atacccaatt tacttgtaga
     3361 ggggattgta gacgctatta aatcgtctgt agctccaaaa gtatatatag caaatataat
     3421 gactcaacca ggtgagacag aagggtacaa tgtattagag catgttaatg ctatagtaaa
     3481 acacactgat gagaatttaa tagattatgt aatcgcaaat aatgagatat tgccagaaga
     3541 aatgcttgac ttatataaac aagatggagc agagcaagtc ttgttagata aaaagcaaaa
     3601 agataaatta aaagagatgg gtataaaaac tgtagagaag aatttaattg aaataaaaaa
     3661 taattatata agacatgatg caaagtatat atctaacata gttattgagt tagccctaaa
     3721 tcataattac aacaaaagtt agtaaaaaag gattttaaaa aattgtgtag aaatccttat
     3781 cattatgtta gttttaagga gtgaacatcg atgagagaag aggttagtgc tggtggtgta
     3841 gtcctgtttg gcaatacgat tcttttacta aggaagttca atggagattg ggtgttaccc
     3901 aagggaaaag tagaagaagg ggaaaataac caagaggcag cattacgtga agtaagtgaa
     3961 gaaactggag taaaagcaga tatattgaaa tatttaggtg aaatacacta tacattcaaa
     4021 gaaaactggg atgaaaatag agcagttcat aaaacagttt tttggtattt gatgcaagca
     4081 aagaatatgg atactattcc acaaaaagag gaaggcttta ttgatgctaa atttatccac
     4141 ctagatagag tagtagactt agctaggtat gatgatgaaa aggagataat aaaggttgcc
     4201 ttacaagaaa taaaaaaaag gttaaagaaa aattagttaa taggtgatag atatgtcttt
     4261 ttcaactgaa accaaaaatg aactagcaag agttatatct gggaatgaat gttgtaatat
     4321 agctgaattg tcagctctag ttaaatcagg gggaagtata caaattgttg gatataagaa
     4381 gctaaatcta aagataacaa cagagttaaa ttcaatagcc cgtaaagtat ttaaattatt
     4441 aaaaaagaat tttaatatca atactacaat ctcagtaaat aagaatcaaa tgctaaagag
     4501 aaataacagt tatgtattaa tggtaacaag cgaaatgggt tcagaaatgt tattaaaaaa
     4561 gttaggtata cttgaagata aagaaggatt ttttactata aacaaaattt cagagaactt
     4621 aattaagcat gaaggatgta agagagcttt cataagagga gcttttcttg gtggaggttc
     4681 tataagcgac ccagaaaaga actatcatat ggaatttgta actaataatg aggagtttgc
     4741 agattctcta aaagaactta taaactcttt aggttttaat agtaaaattg tggctagaaa
     4801 aaataatcac gttgtttata tcaaagagag tgaacaaata tcagatttgt tgagtattat
     4861 aggaggacac cacgctcttt taagtcttca aaatactaaa atagtaaaag aaatgagaaa
     4921 taatgtaaac aggatagtaa attgtgagac agctaacctt tctaaaacag taaatgcagc
     4981 agtaagacaa gtagaaaata ttaagctcat acaagagact ataggaatta gcagtttgcc
     5041 agaaaattta caagagatag ctaagattag aatcgagtat gaggatatga ctctcaaaga
     5101 acttggagag atgttagaac caccaatagg aaaatctgga gtcaatcata gacttagaaa
     5161 aatagaagaa attgcaacag atttgaaaaa aaaatcatag caataaattg aggggacaat
     5221 ctcagaataa gcattaattt ataacatata atggtatatg ttgaagttaa ttcctatttt
     5281 gagataaaag tctctgtttt tgttttagga catatatttg tggtaaaatt aaaattataa
     5341 agcaggaggt gatttaatga acaaagattt tgctcatctt catgtacata ctgagtatag
     5401 tttacttgat gggttttcga gggtgaaaaa acttataaaa agggctaagg aactaaacat
     5461 gagctcaata gctataactg accatggttg tatgtttggt gttatagatt tctacaaaac
     5521 agctataaaa gaaggaataa agccaattat agggtgtgaa gtctatacag ctgcacgtgg
     5581 attgagagat aaagacccaa actatgacaa atatcaagga catttagttc tgttagctaa
     5641 gaatatggaa ggatataaaa atttaataaa aatagtttca acttcatacg tagaaggttt
     5701 ttactataag cctagagttg acatggaaga acttaaaaaa cataacaaag ggattatagc
     5761 actttctgca tgccttgcag gagatgtagc acgtgcttta atgaatagaa attatgaaaa
     5821 agcaaaacaa tttgctcttg actacagaga catatttgga gaagaaaatt tctttttgga
     5881 gattcaagat cacaatttac ctgagcaaaa agaagtaaac tctggtttag taaaattatc
     5941 aaaagagact ggtataccac tagttgctac taatgatgtt cactatgtaa ataaagaaga
     6001 ttcgaaaata catgatgtac ttatgtgtat tcaaatggga aaaacattaa atgacccaaa
     6061 tagaatgaga tttggtagtg atgagtttta tttaaaatca agagaagaaa tggaagaatt
     6121 atttccatat gcattagagg ctattgacaa tactgtgaaa atagctgata tgtgtaatgt
     6181 ggagtttgat tttaatacta tacatcttcc taagtatgat gttccagaag gatatacacc
     6241 agaaacttat ttaagggagt tgtgttttaa tggattaaaa gagagatatg ataatcctag
     6301 taatgagata ctagagagat taaattatga attagatgta attgaaaaaa tgggttatgt
     6361 agagtatttt ttaattgttt gggattttat aaatttttct aaagaaaata atattatagt
     6421 aggaccaggt agaggtagtg cggctggttc gatagtagca tatacactta aaataactga
     6481 tatagaccca ataaagtact ctctattatt tgagcgtttt ttaaatccag aacgtatatc
     6541 catgccagat atagatatag atttttgtta tgagagaaga gaagaagtta tagattatgt
     6601 aaagagaaaa tatggtgatg accatgtagc ccagataata acttttggaa caatgggtgc
     6661 aaaagctgca ataagagatg ttggaagggt attggatata ggctacaata aagttgacaa
     6721 ggtagccaag gaaataccat ttgctttagg aatgacaata gataaggccc tagatacaaa
     6781 tcctaatctt aaagaattgt atgaccaaga cccggaaaca aaagaaataa taaatatatc
     6841 aaaacagata gagggaatgc taagacatgc ttctactcat gcagcaggag ttgttatatc
     6901 aaaaaatccc gtagatgaat atgtaccttt gtacaaacat caagatgcaa tcacaacaca
     6961 gtttactatg acaacacttg aagaacttgg gcttttaaag atggattttc ttgggctaag
     7021 aactcttaca gttataagag atactcttga tttaatagaa aaaaatagag ttataaaaaa
     7081 ttacacagaa aaaatagact tttcaaagat ggattatgac gacccgaaag tatatgaaat
     7141 gctttcatca ggaaatacac taggggtgtt tcaattggag agtgctggca tgagaagttt
     7201 tatgaaacag ctaaaaccag acaattttga agatattgta gcgggaatat ccctatttag
     7261 accaggtcca atggattcta tacctgcata tataaaaaat aaaagcaacc caaaagatgt
     7321 aacatatcta catgaaaaat taaagcctat aatggaagtt acatatggtt gtttggtata
     7381 tcaagagcag gttatgcagg tcgttagaga tttaggtggt tatagttacg gacgaagtga
     7441 cctagttaga agagccatga gtaagaaaaa gatggacgtg atggaagaag aaagacaata
     7501 ttttattcat ggtaaatttg atgaagatgg taatatagaa attgcagggt gtatcagaaa
     7561 tggtgtagaa gaggaaatag ctaataaaat atttgatgat atgatagact tcgctagata
     7621 tgcatttaat aaatcacatg cagctgcata tggggtttta gcatatgaga cagcttatct
     7681 aaaaacatat tatccagttg aatttatggc agcattgata accagtgtaa tgggaaatac
     7741 tgataaggta gttgagtata taagagaatg taaagcacta gaaatagatg tattacctcc
     7801 agatataaac aaaagttttt caaagttttc agttgaaggc gacaatataa gatttggtct
     7861 agcagccgta aaaaatgttg gcgtaaatat aataaacaat ataatcgctg aaagagaatc
     7921 taatggatta tttgtagatt tagtagattt agtcaaaaga ttagaccaaa aagatacaaa
     7981 taagagagta atagaaagtc taataaaatg tggagctttt gataatataa gtgaaaatag
     8041 agctagtctg atggctggtt atgaaagttt attagaaagt gtatctatgg atagaaagaa
     8101 aaatgttcaa ggtcaaattt ctttatttga tgcttttgga gatagtgagg aagatagtga
     8161 ttttcaacaa atatacagtt tgcctaaaag gcaagagtac gaagaaagag agagacttaa
     8221 ccttgaaaaa gaagtcttgg gaatgtatgt aagtggacat cctttatcac aatttgaaaa
     8281 agaactacaa gaaaaaacat ctatggataa tggaaaatta aattcattaa aagaggaaga
     8341 agagtcatac ctacaaatga atgaaacaga tgttataatg ggtggaatga ttgtaactaa
     8401 aactattaaa acaacaaaac gaaatgaaat tatggcattt attgaattag aagatttgta
     8461 tggtgctata gaaataatag tttttcctca attattacaa aagtataatg tcattttgaa
     8521 tgaagataat ataatatatg taaaaggaac attaagtata aaagaaggcg aaaatgcaaa
     8581 acttatagct agagagataa aagatataaa tgatgatact gactttgcag atagaaacta
     8641 caattcagga agaactagaa atgtagaaaa tattaagcca gaaagtggta aaaaattgta
     8701 tttgaaaata gatagtatgt ctgatactga tacaactaat aatattgtag aaatagccaa
     8761 acaatatcca ggtaatgaca gtatatactt ataccctatg gatgaaaata taaatggtaa
     8821 acgtaaagca tataaaatga ccggggcatt gacttgtata tgtgatgaat ttataaataa
     8881 cttagaagag ttacttccta aagataacat aaaagtaaaa gcttgatttt ttatataaaa
     8941 atctaaatat attataataa tatgaaaaat attgtccgtt ttgtgatata atatcacaaa
     9001 atggacattt aaggacattt aattttttta gtcttgatgg gattaaaaag tgatataggg
     9061 acaaaaaaag tccattagaa tacaataaca atgattataa taaaaaattg acataaaatc
     9121 caaggaggta aaaaatgaaa actataggat tactaacaag tggaggagat gctccaggaa
     9181 tgaatgctgc gattagagcg gttgtaagat cagcaatata ttatggatgc aaagtatatg
     9241 gaatcaatag aggatataag ggattactag aagaagattt aactgaaatg aacttatctt
     9301 ctgttggaga tattatacac aggggaggaa ctatattaaa atcttccaga tgtgaagaat
     9361 ttaaaacaga agaaggtaga ttaaaagcag taaaaattct taaaaagtat aaaattgatt
     9421 gcttagttgt aattggtgga gatggttcat ttgcaggagc ccaaaaactt agtgacttag
     9481 gattcccagc tataggcata ccaggaacta tagataacga tttagcatat acagattata
     9541 ctataggatt tgatactgca atgaatacta ttatagatgc tataggtaag ataagagata
     9601 catcatcatc tcatgaaaga gttaacatag tagaagttat gggaagacac tgtggagatt
     9661 tagctttata tgcaggtctt gcaggaggag cggaaactat aatagtacca gaagtagaaa
     9721 ttacagtaga tgaagttgct ctaagattaa agactactca aaaaagaggt aagagacata
     9781 gtataatcgt tttagcagaa ggagtcggaa gtgctagtga tttagaaaag gaacttaaaa
     9841 aagagtcagg agcagattta agagtcactg tattggggca tgtccaaaga ggaggaagcc
     9901 caactgtatc tgatagaata ctagcaagta gacttggagt aagagcagta gaattattat
     9961 tagatggaaa atcagctaga gttgtaggaa taaaagaaaa caaaataatt gatttagaaa
    10021 taagtgaagc tttagctcaa aagaaagtat ttgataaaga agcttatgag atggcaaaaa
    10081 tattatctat ataagtttta gtattaaaaa taaatctgtg gaataatatc tcaggaggag
    10141 caaatatgtt aaataatgta aagaagacaa aaattgtttg tacactaggt ccagcatcac
    10201 aaagtgagga agttttaact caacttatgc aaaatggtct aaatgtgtgt agatttaact
    10261 tttctcacgg ttcgcatgaa gaacataaag aaagaattga tatggcaaag aaagttagag
    10321 aaaaattaaa taagccagtt gctatattat tagatactaa aggtccagaa attagaacag
    10381 gtaactttga agacccagag gtatttttag aagaagggca aaagttcact ataactatga
    10441 aagatattat ggggactaaa gaaatgtgta cagtaagcta taaaggtctt gctgaagacg
    10501 ttaaatcagg agattctata ctaatagatg atggtttagt tggcttaaga gtaaaagaaa
    10561 taaatggaga agatatagtt tgtgttgttg aaaattcagg aatagttaaa aatcataaag
    10621 gagtaaatgt acctggtgtt aagataaatt taccagcaat aactcctaaa gatataagtg
    10681 atatagagtt tggaataagt caaggaatag attatatagc tgcttctttt gtaagaaagg
    10741 catctgatgt actagcaata agagaagtat tagagaacaa taatgcaact gatatacaaa
    10801 taatatctaa aatagaaaac caagaaggtg tagagaacct agatgaaata ttaaaagttt
    10861 ctgatggtat aatggtggct agaggtgact taggtgttga gataccaact gaagagatgc
    10921 caatagttca aaaaatgatg ataaaaaaat gtaatgaatt agcaaagcca gttgttactg
    10981 ccactcaaat gcttgattct atgataagaa atccaagacc aacaagagca gaagttacag
    11041 acgttgccaa tgctatatat gatggaacag atgcgataat gttatctgga gaaactgctg
    11101 ctggaaaata tcctgtagag gcagtaaaaa tgatggctac aatagcaaaa agaacagaag
    11161 agactttaga ttatgataga ttactaaagg aaaatggtac taacaatgtt actgtaacag
    11221 atgcaataag ccatgcaact tgtacaacag cagtagattt aaatgcatca gctataataa
    11281 catctacttc ttcaggatat actgctagaa tggtatctaa attcagacca aaatctccaa
    11341 taatagctac tacaaataat gaaaagacta tgaataaatt agctcttaca tggggagtat
    11401 atccagttaa gtcttctgtt gcaggaaata cagatgaagt tatagaaaag gcaattgagg
    11461 cagctagaca agctgattat atagataatg gggaattagt agttataact gctggagtac
    11521 cagtaggagt aagtggaact actaatttaa taaaggttca tgtaataagc gaagaaatcg
    11581 tgcaaggtat aggtgtaggt actcaaacag tagaaggtaa agttcgtata ataaagaatg
    11641 gaaatgcttg tgtagacttt aatgaaggcg acatacttgt ttctactgct actgatgctg
    11701 aaatgaacaa ttatatagaa aaagcaggag ctgtagtaac tgagaatggt ggaatgacat
    11761 cacatacagc tattgtaggt ataaatttag atataccagt tattgtgtct gcagctgata
    11821 taacaaatct agtaaaagat ggagaagttg taactgtgga tgcatcaaga ggcgtggtat
    11881 atagaggttc aactagagtt ttataagaaa aataataaat ttatttacta aggggcttta
    11941 taagctcctt tttttttggt aaaataaaat attgtagctt agtaaaaaat atagtttaat
    12001 taatagcaat attttaatat aaatttaaag aaaaaagttt gtaaagattt aaatataata
    12061 gattgattgg agggtgattt atgctttcga aaaacgagat gtatattgta gatatagtag
    12121 acattggtca aggcggagta ggagtaggaa agtacaatgg tattacagtt tttgtagagg
    12181 gcggattaat acatgataaa gttaaagtta ggattaataa agtaaagaaa aattatgctg
    12241 tgggagatat tgttgagata atagaaaagt ctccattcag ggtgaaaaga gtgtgtagtg
    12301 acaatcttag ggattgtgga gggtgtcaga tacaagactt agattataat aaacaacttg
    12361 aaatgaaaac aaatgaagtt aaacaagtac tttcgagaat aggtaagtta gaggatgtag
    12421 aagttcatga cactatagga atggataatc catatagata tagaaataaa gcacaatttc
    12481 caatacaaaa gaataatggt attccaatta ttggcttcta taaaaagaag actcatgatg
    12541 taatatcgac agaaaaatgc gtaatacaac atgagataaa tgataaaatt gttaaaataa
    12601 taaaaacata tataagggcc tataaggtaa gcatatatga tgaaaagact cacacggggt
    12661 tgattaggca tttagtaaca aaaataggtt ttacaactaa agaagtaatg gttgtattag
    12721 tagcaaatgg aaacaaactt ccatatctta aagaattagc ttcagtgctt aaggaaaata
    12781 tacctggatt taagactttg gttgtaaatg taaatcaaaa agatacgaat gctattctag
    12841 gaagagaaaa tatagttata tatggagatg gaaaaataaa cgattatata ggagacttag
    12901 tgtttgagat ttcaccatta tcatttttcc aagtaaaccc tgtccaaact aaagtattgt
    12961 atgataaggc tcttgaatat gcaaatttaa atgatactga tactgttttt gatatttact
    13021 gtgggatagg tacaatatct ttatttttgg cacaaaaggc taaaaaggtt tatggaatag
    13081 aaattataga tgatgccata aaagatgcta agataaatgc taaattaaac aatatacata
    13141 atgctgaatt ttatgttgga aaggcagagg aaattgtacc taagatgtat agagagggta
    13201 agactgccaa tgtagtcgtt gtagacccac ctagaaaggg atgtgacgaa aaggtattag
    13261 agactattgt ttcaatgaat ccggaaagaa ttgtctatgt ttcttgtaat ccttctactt
    13321 tagcaaggga tttagattat ctaaatgttc acggatttaa atgtgttgag gcacaacctg
    13381 tagatatgtt ccctcattct acacatatag agacttgtgt gttgctacaa agacgaacta
    13441 tgtagaaatc cttaatttta ggcacttttt taagcatata gtctttacga gcgagggcga
    13501 taaaatcaga aataaagaga taaaaaacta tatcaatgtt tcagtagttg ttgatataga
    13561 gtgtgggaac acaaacaatt aaatattaga tttgaggggt acttgtaaaa agtatccctt
    13621 ttcaatgtaa gacgttcaat attgagcgtc tattttttta cccaaaatga aaggagcatt
    13681 tagaattatg aaaaagatat tgaaacgatt gtgtacggga gtacttgcct ttgcgactgt
    13741 cgttactgct ttaccgagta ccgccgtcca tgcagaaagc aagcaatact ggacggaatc
    13801 agaggaacgt gtcggtatcg tagaaaaagt aatgaatgac ggttcgattg gttctacctt
    13861 taacgaggga catttgactg ttgagggcga ggacgcttac tgtatagaca tcaatacaga
    13921 ttttaagaat ggctacaaga ccagagctga cgcaagcacc cgtatgagtg ccgaccagat
    13981 ttcagatgtg gcgttatcct tagagtatgt caaacagtat ggcgaagcac acaaggaatt
    14041 gaactacaag caagtctatc tgttggaaca atgtgttgtc tggcagagat tgagcgtaca
    14101 tcttggctgg caatgcgaca acgtgcgagc ttcttatgat gaaataccaa aagcaactca
    14161 ggacgaagtt ttcgctggtg ctaaagcctt tgtgaaagaa aataaaggac gctatgaatg
    14221 tggcggttat atctactctg gcgaggggca ggaattggga caattctggg cgaagttaaa
    14281 tgtcggaaat gcaaaactgc aaaagacttc cagtaatacc agcattacag acggtaacgg
    14341 gaattactca atcgctggtg cgacctatgg tgtcttttct gataaggact gcacgaaaca
    14401 gcttgccacc cttacgactg ataaaaacgg aaatacagat gttgtagagg taaaagcagg
    14461 tacagtctat attagggaat tatccgcacc agcaggatat aaagttgata caaccgtgta
    14521 ttccttaaaa gttgaagctg gaaagacagc gaccttgaac gtatcagata caccaaaagt
    14581 aacggacacc ttgattgagc ttttcaagat tgatatggaa acgcagaaag acaatccgca
    14641 gggaaacgct tctttagagg gtgcagagtt tacatggaag tattatgctg gcttctataa
    14701 caaagacaat ctccctgccg aagctactcg tacatgggtt acaaagacaa tcgctgaaac
    14761 agacagtgac gggacaactc actacatcac aaaattagcg gacgcttaca aggtatctgg
    14821 cgacagcttc tatatgcagg acggcaaagc gattcttcct ttaggaacac tatctgttga
    14881 ggaaacaaaa gctccaaacg gctacttgct ggacggtgca tatatgcagg ctggcgataa
    14941 gtctgaacag ataaaaggct tataccttac acaaattaca gaggacggcg accttgctgt
    15001 attgacggga agtaaccagt tttccgtatc agacaaggtt atccgtggcg gtgtgaaaat
    15061 tcagaaaaga gatttagaaa cgagcgatac caaaccacaa ggaagtgcca ctttgaaaga
    15121 tactgctttt gacgtcattt ccttaaatga caattctgtt ttggttgagg gcaagctata
    15181 taagaaaaat gaagtggtaa agacaattcg tacagatatt gagggtgtgg cttctacttc
    15241 tgctgacctt ttaccttatg ggaaattccg tatcgttgag agtgaagctc ccgacggtta
    15301 cttgacagac ggtgcgaagc cgattgactt tgcaatcaca gagaatggaa aaatcgtgga
    15361 cttaaccgac gaagcccact ctatctacaa tcagattaag cgtggggata ttgagggagt
    15421 gaaaatcggt gcaggcacac acaagcgtct tgctgatgtt ccctttagga tcacaagcaa
    15481 gacgacggga gaaagtcatg ttgtagttac tgatgataac gggcaattct ccacttctgc
    15541 tgaatggact tctcataagc acaatactaa cgcaggcaag accagcgagg acggtgtgtg
    15601 gtttggaact tctgaagcag acgacagcaa aggtgcattg ccttatgata cttacatcat
    15661 tgaagaaatg cgttgcgata gtaacaaagg ctttgaactt atcccacctt ttgaaatcgt
    15721 ggtatcaaga aataaccttg tgattgattt agggactttg actgatgaat acgaaaaaga
    15781 aatctctatc cataccacag cgaccagcaa agacagcgaa aagactatcc ttgcaggaaa
    15841 agaggtaaca atcgtcgata ctgtcaaatt agacgggctt acaaaaggta caaaatatca
    15901 gctcaaaggc tggcagatgt taaaggaaga aaatgcagag cttatcattg acggaaaacg
    15961 tgtggaaaat gactatactt ttatcgctga tgatgaagaa atgaaagtgg aaatttctta
    16021 tacattcaat gcgtccgagc ttggcggtaa aaatctggta acattcgagg aattatacga
    16081 tttaagcaat ccagacgaac ccgtgaaagt tgcggaacac aaagacattg aggacgacgg
    16141 acaaactgta ttgattacag aacgtatcat caagatacat acgactgcta ccgacaaaga
    16201 cggcaacaaa gagattgaag ctggaaaaga cgttacaatc attgataccg taaccttaga
    16261 gggcttggaa gtcggtacac agtacaaact tgtaggctgg cagatgttga aagaagaaaa
    16321 tgcagaactt cttatcaatg ggaaacgtgt ggaaagtgat tacaccttta ccgctgacag
    16381 cgaaactatg aaagcggaag ttgcctttac ctttgacgct acttcccttg acggcaaaca
    16441 gcttgtaact tttgaagaat tgtatgattt aagcaatcca gacgagccga agaaagttac
    16501 cgagcataag gatattgagg acgagggaca gacgattact tttaatgaaa aaccagaagt
    16561 cccagagaaa ctcgaaacac cgcagacacc agaaaagcca aacagaccta gcgacagtcc
    16621 taaaacgggc gacagtacaa atgtcatggc atttcttgtg atgttgctta catctgctgg
    16681 tggactggct ggaacatatc tttacaaacg ccgtacaatg aaaaaatcat aaggcggtaa
    16741 cggtatgatg aaagaaaaat cacgttctcc gccgaaactg tcaaacggca gacaagccag
    16801 aataaaaaat tacaatcata aacttatcaa atatagaaag aaacgaggta aaacaatatg
    16861 gaaatgaaat atgtcgtgcc agatatggca cagtcttttg gaactcttga atttgcaggc
    16921 gaaagcgagc ctatctttga aagggataaa aacaaccgca aggtcatagc cagacgaagc
    16981 tataaccttt attctgacgt acagaaaggc gaaaatgttg tggtggaaat tcccgtgcag
    17041 gctggcgaaa agcatttcaa gtatgaacag aaagtaaaac ttgtcaatcc gaagttatac
    17101 ggcagagggt acgcaatcgg ggatgtggga cataccgatt atgtattagt agctgatgat
    17161 attgtagcag ttgaagaaaa gtaaaggagt gaagaattta tgagattatc aaatggattt
    17221 gtcattgaca aagagaaaac atttggagaa ttaaaattta cagcggtgcg tgatgtgttc
    17281 ttgcaaaatg aggacgggac accgagtacc cagcttaaaa agcgtatcta tgatttgaag
    17341 tgcagtctgc atggtggaat tatccccgtg tccgtaccgc cagaagtacc gttaagggaa
    17401 tttccttaca atgcagtcgt ggagcttgtc aatcccgtag ccgatacggt atcacgaaaa
    17461 acctatacgg gtgcagatgt ggactggtat gtaaaggcag aagatattgt actgaagaat
    17521 aaaggcaatc agaacacagg cacttcacag aaccatacac cgcagggaca accgaaaaaa
    17581 tagacttgct gaatagattt tcgttgtaga ggatatacgg aactgttata atatgggtat
    17641 gaagaaagcg acaaattaga atttgataag gagcgaaaag aaaaatgtgt gaagaatgtt
    17701 actctgatga aaatagaata acaccattac taaatccctt ggactgctta gaaaaccaca
    17761 ctcaatatat ttgtggaact tgtggacgtt gcatttgtat tgaacatgac ccaaacagag
    17821 gattacaaag atggaatttt ccttttaagt cattggaaat tgcaaaactt tatttacgaa
    17881 cagccgatta tacaacgaaa agttcatgtg gtatttatga gatagagaac agtaaaggca
    17941 gagtatctta taaaatattc gccgggaatg aggatttaca tctattcttg aaaaagaata
    18001 aagacaagaa atgcaaacaa atgacacctg tgtttaatgt tggagagtat aaagaatatc
    18061 cacatacaga agtacgaaaa ttgacttctg atgaaataaa acaatatatg agtgaacgtt
    18121 aaattccagt ttgacaactt catatccatg cacacaaagc agttagtcta tacgattgac
    18181 tgcttttttc atacccagaa aggagtgatt gatttatgag aatgatttta aacaaaggac
    18241 accgtatcag agccagcgat aagaaccttg tctatcgctt ttcaatgggg acactcctgt
    18301 ttgtgttcgt agcggttatc ctgctattaa atacaaaaca gctcatgcgt accgactggg
    18361 aacatttcag cttattagac aatggcttta cgctttccct ttacaacttc ataaccatac
    18421 tgatagcgac tggtgtttgt gcattggtcg cttttctgta ttaccgcttc tgttacgaca
    18481 gtttcaaaaa gctactgcac cgtcaaaagc tggcacgaat gatactggaa aacaagtggt
    18541 atgaagctga taccgtacaa gacagcgttt tttttactga cctgcaaagc agatcaaggg
    18601 aaaaaatcgt ctggtttcca aagatttatt atcaaatgga aaagggactg cttcatatcc
    18661 gctgtgaaat tacgctgggg aaatatcaag accagctttt acggttagag gataaattgg
    18721 aaagtggctt gtattgtgag ctgaccgaca agaccctgca tgacggctat atcgaatata
    18781 ccctgcttta tgatatgata gcgaaccgca ttaccattga tgaagtacgg gcagagaacg
    18841 gctgtcttag actgatgaaa aatcttgtct gggaatatga cgcactccct cacgctctga
    18901 ttgctggtgg gactggtggc ggtaaaacat attttcttct gacgctcatt gaagccctac
    18961 tgcataccaa cgctgtcctt tacatcttag acccgaaaaa tgcggactta gcagacttgg
    19021 gaacagtcat gggaaacgtg tatcacacca aagaagaaat gatagactgt gtcaatgctt
    19081 tttatgaggg catggtacag cgaagcgagc aaatgaaacg acacccgaac tataagacgg
    19141 gcgaaaatta cgcctatctg ggactgccac cctgctttct tatctttgat gaatatgtgg
    19201 cgttttttga aatgctgggg acaaaagaaa gtgtgggctt acttagccag ttaaagaaaa
    19261 tcgtcatgtt aggacgacaa gcaggttatt tccttatcgt tgcctgccag cgtccagacg
    19321 caaaatattt ctcggacggt atcagagata acttcaattt acgtgtggga ttggggcgta
    19381 tcagcgagtt aggttacggt atgctgtttg gttcagatgt gaaaaagcag tttttccaaa
    19441 agcgtatcaa agggcgtggc tattgtgatg tgggaacaag cgtcataagt gaattttaca
    19501 cgcctttagt cccgaaagga catgattttt tgcagacaat cggttttctt gcacaagcaa
    19561 ggcaggacgg gacggcgacg tgcgaagcga aaggcgacgg ctcggactag ccgttgctgg
    19621 tgtggcgtaa gccacgccag caggtaaaac ccctcggtat ctaacagagg ggtacgtttg
    19681 caaatagaca atagacaaaa aaacatagga aacataaggc ttctggcatg gtttcctagc
    19741 aaaaatcggc tgtgaagtcg ccttaaaaaa cttcacactt gacagattgg gggtatggtt
    19801 ctgaatgaag aacaatggat aaaagaatta cgggagaagc ggattgctta cggtatctca
    19861 caaggcaggc tggcggtagc ttctggtatc acaagggaat atctaaacaa gatagaaagc
    19921 ggaaaaatga agccgtcaaa agaacttctg gaaactttgc ataaagaact ggcaaggttc
    19981 aatccagaag caccgcttac catgctgttt gattatgtga aaattcgttt tcccacgctg
    20041 gatatacagc acatcatcaa agacatatta aaactgaata tcaattatat gctccatgaa
    20101 gattacggac attacagtta tacggagcat tattcattag gggacatctt tatctatacg
    20161 tcggctgacg aagaaaaagg tgtcctttta gagttaaagg gtcggggttg caggcagttt
    20221 gaaagttacc tgctggcaca gcaaagaagc tggtatgact ttcttatgga cgcactcata
    20281 gacggaggca ttatgaagcg tatcgacctt gctatcaacg accatacggg cattttggat
    20341 attccagagc ttgcggagaa atgcaggaaa cgggaatata tcggaaagtc cagaagctat
    20401 aagtattacc agtcgggcga gcttatcaag cacagagagg acgacagaga atatatgggg
    20461 agtacccttt atcttggttc gctgaaatca gatgtgtatt tctgtatcta tgaaaaggac
    20521 tatgagcagt atgtcaaatt aggtacacct ctggaagaag ccgacattat caaccgtttt
    20581 gagatacggc ttagaaatga acgtgcttac tatgcagtac gagatttgct gacctactat
    20641 gacgcagagc agaccgcttt ttctatcatc aaccagtatg tgcggtttat tgatgaagaa
    20701 ccagacaagc ggaaaaatga ctggaaactc aatgaccgct gggcttggtt tattggcgat
    20761 aatcggcaaa gtctgaaact gacgacaaaa ccagaacctt acaccttaga ccgcacattg
    20821 cgttgggtac aaagacaggt agcaccgacc ttgaaaatgc tgaaaaagat tgataaggga
    20881 aacggtacag actacatgga aacgattgaa aagcaggcaa tcttgagtga aaagcatgaa
    20941 atgataatca aacagcagac gacccctgca aaagatttgg tggaaagtta ggagtgataa
    21001 aaaatgtggg aattagtaaa agacttcctg ctggtatcta tgggaatggg tatcggcgta
    21061 gtcttgatgt gtattttgag tatcagcaaa gaagctgact atgaaatgaa acaattagaa
    21121 gaaagcgagg acaattaaat gaatttcgga caaaatttgt atcaatggtt tttatcaaat
    21181 gcacagagct tagtgcttat ggcgattgtg gttatcggta tctacttagg gttcaagcgt
    21241 gagttttcaa aacttattgg cttcttggta gttgccctta ttgctgtcgg cttagtattc
    21301 aacgctggcg gtgtgaaaga tgtactgtta gagctgttca ataagattat tggagcataa
    21361 cgaattttat tgttgctgta atatgagaat gttataatgt gggtacgata aattattttt
    21421 tgaaaggaat aaaagtgctt atgatttcag atgaaatgag agtggatatt ttacatgcag
    21481 tattcataaa caaaaatttt attaaagatg aagaaaattg taattatgaa aaatacttgt
    21541 tggaattagt taataaatca atatatttta gagaaaaatc aaattttgca gaatatgtac
    21601 cacctaaatc tgaaaatcat ggagaatgtg attgtaactc acctaattat caaatggact
    21661 ttaaattact tgaaagcaca acaagacttc atgcttcaaa agaacttact ggtcaaatac
    21721 agaaattctg tgatggtgta attggtaagt gtccaccgag aagaccaaat acacaaatga
    21781 ctgtaactag gttatttgct tcgttgcggg attatgattg tgaaagttta cattcttgtc
    21841 ttacagagaa atatgagtat ggaacgattg aatttgatat acaaacctat gtgaaattgc
    21901 ttactttcaa gaaaaactta tttttctttt ttccatacaa gtttagtttt aatacttgtt
    21961 ataactttaa atatgcttta gacagtataa gaattgcttt agaaaaagat tttagagagt
    22021 caaatctttt tagagaaaaa tactatgcag agtatgatac tttcttagca tatatctatg
    22081 aagataatct gataataagt aaatttgaac aagacggcaa gttacaaatg attgattgta
    22141 tatatttatt taaaagtcaa acatacagta aactttacga gtacacatgg taaatcagtt
    22201 acacttaaac aaattcatac ttacacacaa agcagttagt cttaggattg actgcttttt
    22261 tcttacctaa atttcagatt ggagtgatga aatgaacgat atttttaaag atatgcaggc
    22321 aaaagtcggc tgtgaataca tttccgacct gccctcttac aagcgtaagg tgtggcatga
    22381 aatgaaacgg ctgaaccctg ccgactatga agaaaaacag ttgaaagatt tttctaaata
    22441 tgtgtttggt atgtcatacc agaccttaaa agatgtgatg aaacaacaga aaggacgtga
    22501 ggaacaatgc aggaaacaag ggtactggtg gaaacgggaa gaacaaatgg cgaagaaaca
    22561 acatcatact ggtttgactt accgatagat gttgcagagt ttgaagaaaa gttaggtgtc
    22621 ggtgcagaaa gtcaagatta ccgcattatc gaaaaggtgt tgccttatgc tgatgaagtc
    22681 cacgaacaca ccagcgtgta ccagcttaac gaattagatt ttatgtaccg ccagctatca
    22741 gccgatatgc aggaagaata tgtagaactt cttaccgtgt ttgagaactt agaagcactt
    22801 tatatttgca ggaacgtgat taccgtttat cacgactgta aaagcatgat agatgttgca
    22861 aggcaaaagc tgatgaacga cccgacattc aaacatttat ccgaggactg tcaagcatac
    22921 tatttcgatt ttgaagccta cgcttctcac ttacttgaaa acaaacgcta tcttgtaacg
    22981 gaaaacagta tctttgaact gccagaatag gaaatgaggt ggtgcttatg attgatgata
    23041 tggcggttta tattgctaat cttggcagat acaatgaggg atatttagtc ggtgcgtggt
    23101 tcactttccc tattgacgag gaagatgtaa aagaaaaaat cggcttgaat gaacagtacg
    23161 aagaatacgc tgtccatgat accgacaact ttcctatcga cataggggaa tatgtttcca
    23221 ttgaagaact caatgaaatg tatgaaatga tagaggaact tcccgactat atcgtagagt
    23281 gtctggacga atttatcagc cactatggga cgctggaaga agtcgtggaa cataaggacg
    23341 atatttattt ttatcccgat tgtgaaacca tgacagatgt tgcctactac tacatagacg
    23401 aattgaacgc acttggggac attccagcta acttacagaa ctatattgac tatgaagcct
    23461 acgggcgaga tttggatatg ggtggtcgat tcattgaaac aagccgaggt atctgtgaga
    23521 taccatatta acgctggaag atcagcaaca agcattgttg ctactgacag cgtatttctc
    23581 tttaagggac agtctgaaaa tggctgtcct tttctctttg aagaacttac gaaaggggct
    23641 gaaagaactt gaaaaagatt aaaagctaca cgggtatctg gaacgtggaa aaagtcttat
    23701 atgcaatcaa tgactttaac ttaccctttc ccgttacctt tacacagatt acatggtttg
    23761 tgattacgga atttatcatc attctgtttg gggatatgcc cccactttcc atgattgagg
    23821 gagcatttct caaatacttc ggtattcccg ttgctctcac ttggtttatg tcgcagaaaa
    23881 cctttgacgg aaagaagccg tacagctttt tgaaatcaca gataacctat gccttgcgac
    23941 ctaaaatcac ttatgcagga aaagccgtaa aactgcataa gcagatattg aatgaaacaa
    24001 tcacggcagt aaggagtgtg aactatgttc ccgataaaat atattgacaa taaccttgtc
    24061 tggaacaagg acaatgaggt gttcgcctac tatgagatga taccgtacaa ctattctttt
    24121 ctatccgcag agcagaaatt tatcgtgcat gacagttttc gacagctaat cgcacagtcc
    24181 cgtgagggta aaattcatgc cttgcagatt gccacagaaa gctccattag aagtatgcag
    24241 gagcagtcaa agaaactggt aacaggcaaa cttcgtgagg tggcagtcca aaaaatagac
    24301 gaacagaccg aagcgttagt atcaatgatt ggggacaatc aagtggacta ccgctttttt
    24361 cttggcttta agctcatggt tacggaagaa cagctcaatc tgaagaacat caaaaaatcg
    24421 gcatggctga cgttcaagga atttcttcat gaagtgaacc acacgctgat gaatgatttt
    24481 gtttccatgc cgaatgatga aatcaaccgt tacatgaaaa tggaaaagtt actggaaaat
    24541 aaaatctccc gtcgctttaa ggtgcgtcgc ttggaaatcc atgattttgg gtatctcatg
    24601 gaacatcttt acggcaggga cggtatcgcc tatgaagatt atgagtacca gctaccaaag
    24661 aagaaattga agaaagaaac gctgataaaa tactacgacc ttatccgtcc gacaaggtgt
    24721 gtgattgagg aaagccagcg gtatttacga ttggaacatg aggacaagga aagctatgtg
    24781 tcctatttta ccgtcaatgt aattgtcggg gagttggatt ttccgtcctc tgaaatcttc
    24841 tatttccagc aacagcaatt cacattcccc gttgatactt ctatgaatgt agaaatcgtg
    24901 gagaaccgta aggcattaac gacagtccgc aacaagaaaa aggaattgaa agatttggat
    24961 aatcacgcct atcaagcagg aagtgaaacc agctcaaatg tggtggacgc tttagacagc
    25021 gtggacgagc tggaaaccga tttagaccag agcaaagaaa gtatgtataa gttaagctac
    25081 gtcatacggg tgtctgcacc cgatcttgac gagctgaaac gccgttgtga tgaagtcaag
    25141 gacttttacg acgacctcaa tgtaaaactg gtgcgtcctg ctggcgatat gttgggactt
    25201 cattctgaat ttttacctgc cagcaagcga tatatcaatg actatgtgca gtatgtaaag
    25261 tcagacttct tggctgggct tggttttggt gcgacccagc agttagggga aaacacgggt
    25321 atctatatgg gctattccgt tgatacggga agaaatgtgt acctgcaacc gtctttagct
    25381 tcgcagggag taaaaggcac agttactaat gcccttgctt ctgcttttgt gggaagtctt
    25441 ggcggtggaa agtcattctg caacaatcta cttgtatatt atgcggtgct gtttggcggt
    25501 caagcacttc tgttagaccc taaaagtgag cgtggcaact ggaaagaaac gctcccagag
    25561 atagcccatg aaatcaatat cgtaaacctt accagcgaca aggacaatgc aggacttctt
    25621 gacccatttg tgattatgaa gaatgtgaaa gacgctgaaa gtctggcaat cgacatctta
    25681 acattcctta cgggtatttc ctctagggac ggcgaaaaat tccccgtgtt acgaaaagcg
    25741 gtgcgttctg ttacccagag tgaaaataga ggactgttac acgttattga ggaattgaga
    25801 aaagaagata ccgctatctc acgcaatatc gcagaccata tcgacagctt cacggactac
    25861 gactttgcac acctgctgtt ttcagacggg acggtagaaa atgctataag ccttgataac
    25921 cagctcaata tcattcaagt agcagacctt gtactgccag ataaagatac cacttttgag
    25981 gaatacacga ccattgaatt attgtcggtg tctatgctga ttgtgattag tacctttgcc
    26041 cttgatttta tccattcgga cagaagcatt tttaagattg tagatttgga cgaagcgtgg
    26101 gcgttcttaa atgtggcaca aggcgaaacc ctatcaaata agctggttcg tgctggacga
    26161 gctatgcagg caggcgttta tttcgttacc cagtctgcct atgacgtgtc aaaggaaagt
    26221 ctgaaaaaca atatcggttt gaagtttgca tttcgttcta ctgacatcaa cgagataaag
    26281 cagaccttag aatttttcgg tatcgacaag gacgacgaaa acaaccagaa acgcttgcgt
    26341 gatttggaga acggacaatg tttattgcag gacttatacg ggcgtgtcgg tgtggtgcag
    26401 atacacccag tctttgaaga attactacac gcctttgata ctagaccgcc cgtacagaga
    26461 aatgaggtgg agtgatgaaa gaaaggatac agagtgcgtt cacaaaaagg aagatacttc
    26521 actttctcaa aatggctctg ttcgtggtgg cactttccct tatcctgctt tcacttctgg
    26581 ggacggtggc tcatgcgacg gggcttgtgg acgataccat aaacgcagaa aatctatatt
    26641 caaaatatcc gctttccaac taccagcttg attttttcgt cgataatagc tggtcgtggc
    26701 ttccgtggaa ctggctggac ggtatcggaa aatcggtaca gtacgggctt tactgcatta
    26761 ccaactttgt ctggacgata agcctttatt taagcaatgc aactggctat gtggtgcagg
    26821 aagcctataa acttgatttt attaacgata tggcagacag tatcggaaaa agcatacaga
    26881 cccttgcagg cgttaccgag aacggctttt cttctacggg cttctatgtt ggtttcctgc
    26941 ttcttattat cttagtggtg ggaatgtatg ttgcctatac gggactgata aaacgggaaa
    27001 ccagcaaggc acttcacgct gttatcaact ttgtggtggt gttcgtgctg tccgcttcgt
    27061 ttattgccta cgctcccgac tacatcaaga agataaatga attttcatca gacatcagta
    27121 ccgcttcgct tgatttggga acaaaaatca tgctccccaa ctctgacagc gagggcaagg
    27181 acagcgtaga cttgatacgg gacagcctat tttctattca agtacaacag ccgtggctac
    27241 tcttacagtt tggaaacagc aacgcagagg aaatcgggac agaccgtgtc gaagctctcg
    27301 tatcggcaag tccagaggac gaggacggaa agaccagaga agaagtcgtg aaaacagaaa
    27361 tcgaggacaa cgacaacaac aatctgacga taccgcaggt ggtaaaccgt ttaggtatgg
    27421 tgttcttcct cttgttcttt aatcttggta ttacgatatt tgtattcttg cttacgggta
    27481 tgatgttgtt cagccagata ctttttatta tcttcgctat gtttttacct atcagctttc
    27541 tactttccat gataccgagc tatgaaagca tggcaaagca ggcaatcgtg agggtattta
    27601 ataccataat gacacgggca ggaataacgc tcattgtaac ggttgcgttt agcatttcca
    27661 gtatgtttta taacatatcc acggactatc cgtttttcat ggtggcgttc ttgcagatag
    27721 tatgtttcgc tggtatctat atgaagctgg gcgacttaat gagtatgttt tctctcaacg
    27781 ctggcgacag tcaaagcatg ggacgcagga tattccgcag accgtatctg ttcatgcgac
    27841 atcgggctag gcgtatggaa caccgtattg caagggctgt aagtgctggc ggtatttcgg
    27901 gcggtgtggc tggtgcggta gctggaagtg ccgttgctgg caagagagcc gaaagaaaaa
    27961 atacggcttc taaagaaaat cggggcaata ccacttccag tatgggacag cgtgcaggct
    28021 caaaggtggg tgctgtctta gatacgaaaa ataaagtgaa agacaaggca aacgctgtca
    28081 aagagaatat caaggatatg cccacacaga ccgcttatgc ggtgtattcc gcaaaggaaa
    28141 aggcaaagtc cagcgtgtct gatttcaagc gtggtatggt gcaggaacag cagtccagac
    28201 agacgggacg cttggaaaag caggaacagc ataagaaaag tatcgctgtc aagcgtatgg
    28261 agcttcaaaa ggcacaagaa gcaaggcagg cacagcgaaa ggctgacgga tcagcgacaa
    28321 cgggagctac ccgtccccat gagcgaccag ccacagcttc aaccgtttca aagacgggtg
    28381 cggaaaaaat gcaggaagtc aaacgccctg ccacaactcc gactacgaaa gcaagcgaac
    28441 cagttaagac aagcggtgtc aaagaacgtc cgctatcttc tagtactcct gacgggaaag
    28501 cgacccaatc gacatatagg cagaatgtag aaaaagttac cgtgtcaaag gaaacacgcc
    28561 agaattacac caaagaccgt aggacaaagg ttcagcagac ccagactgtc cagaagaacc
    28621 agcagaccac agagaaaatc cgtaatcttg taacgaagaa aggacagaag aaaaaatgaa
    28681 actgaaacat atcgctatca ttggaagtct gtttcctatt ctcttttctc tggtgctttt
    28741 ctttggggtg ctgattagtg cggacagcga cgacgaaaac agcaactttt cttctggcat
    28801 tacgggtatg aacttatccg cagaagtttt gaaacatcag actatggtag aaaaatacgc
    28861 cagagaaaac ggtatctccg agtacgtcaa tgtactactg gctatcattc aagtagaaag
    28921 tggcggtacg gcagaagatg ttatgcagag ttcagaaagt cttggtttac cgcctagttc
    28981 cttagatacg gaaagctcaa tcaagcaggg gtgtaagtat tttgcgtccc tgctttcttc
    29041 ctgcaaaaat caagatatcg acgatttgaa tgtagcgata cagtcctata actatggcgg
    29101 tggctatgtg ggctatgtgg caggaaacgg aaagaaacac acttttaatc ttgcagagag
    29161 cttcgcccgt gagaaatcgg gtggaaagaa agtaacctat accaacccga tagccgttgc
    29221 gaagaacgga ggctggcggt atggctatgg aaatatgttc tatgtggaat tagtcaatca
    29281 atatttgacc gtggcacact ttgataatgc gacggcacaa gcgattatga atgaagcgtt
    29341 gaaatatcaa ggctggaagt atgtgtatgg tggcagtaac cccaacactt cctttgattg
    29401 tagcggactt gtgcaatggt gctatggaaa agctggtatc tccttaccga gaacggcaca
    29461 agcccagtat gacgctaccc aacatcttcc actctcgcag gcaaaggctg gcgatttggt
    29521 atttttccat tccacctata acgctggttc gtatgtaac
//
LOCUS       DBGJFU010000041        29358 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.041, whole genome shotgun sequence.
ACCESSION   DBGJFU010000041 DBGJFU010000000
VERSION     DBGJFU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.041"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            345..1214
                     /locus_tag="ACOIAS_001738"
     CDS             345..1214
                     /locus_tag="ACOIAS_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2701451.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            1440..2306
                     /locus_tag="ACOIAS_001739"
     CDS             1440..2306
                     /locus_tag="ACOIAS_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2701452.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            2498..2695
                     /locus_tag="ACOIAS_001740"
     CDS             2498..2695
                     /locus_tag="ACOIAS_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2701453.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            2698..3141
                     /locus_tag="ACOIAS_001741"
     CDS             2698..3141
                     /locus_tag="ACOIAS_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701454.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(3328..3415)
                     /locus_tag="ACOIAS_001742"
     tRNA            complement(3328..3415)
                     /locus_tag="ACOIAS_001742"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3379..3381),aa:Leu,seq:caa)
     gene            3567..6215
                     /gene="polA"
                     /locus_tag="ACOIAS_001743"
     CDS             3567..6215
                     /gene="polA"
                     /locus_tag="ACOIAS_001743"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGZ2701455.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            6224..6826
                     /gene="coaE"
                     /locus_tag="ACOIAS_001744"
     CDS             6224..6826
                     /gene="coaE"
                     /locus_tag="ACOIAS_001744"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGZ2701456.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            6819..7373
                     /locus_tag="ACOIAS_001745"
     CDS             6819..7373
                     /locus_tag="ACOIAS_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGZ2701457.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            7373..9088
                     /locus_tag="ACOIAS_001746"
     CDS             7373..9088
                     /locus_tag="ACOIAS_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2701458.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNITSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            9161..9373
                     /locus_tag="ACOIAS_001747"
     CDS             9161..9373
                     /locus_tag="ACOIAS_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HGZ2701459.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            9574..10173
                     /locus_tag="ACOIAS_001748"
     CDS             9574..10173
                     /locus_tag="ACOIAS_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701460.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            10401..10772
                     /locus_tag="ACOIAS_001749"
     CDS             10401..10772
                     /locus_tag="ACOIAS_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2701461.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            10904..12178
                     /locus_tag="ACOIAS_001750"
     CDS             10904..12178
                     /locus_tag="ACOIAS_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HGZ2701462.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            12289..13233
                     /locus_tag="ACOIAS_001751"
     CDS             12289..13233
                     /locus_tag="ACOIAS_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701463.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            13449..14786
                     /gene="rsxC"
                     /locus_tag="ACOIAS_001752"
     CDS             13449..14786
                     /gene="rsxC"
                     /locus_tag="ACOIAS_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGZ2701464.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            14809..15786
                     /locus_tag="ACOIAS_001753"
     CDS             14809..15786
                     /locus_tag="ACOIAS_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HGZ2701465.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADISSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            15786..16355
                     /locus_tag="ACOIAS_001754"
     CDS             15786..16355
                     /locus_tag="ACOIAS_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HGZ2701466.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            16368..16961
                     /locus_tag="ACOIAS_001755"
     CDS             16368..16961
                     /locus_tag="ACOIAS_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit E"
                     /protein_id="HGZ2701467.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            16976..17551
                     /gene="rsxA"
                     /locus_tag="ACOIAS_001756"
     CDS             16976..17551
                     /gene="rsxA"
                     /locus_tag="ACOIAS_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGZ2701468.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            17567..18538
                     /locus_tag="ACOIAS_001757"
     CDS             17567..18538
                     /locus_tag="ACOIAS_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HGZ2701469.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            18758..19342
                     /locus_tag="ACOIAS_001758"
     CDS             18758..19342
                     /locus_tag="ACOIAS_001758"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HGZ2701470.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            19387..20046
                     /gene="radC"
                     /locus_tag="ACOIAS_001759"
     CDS             19387..20046
                     /gene="radC"
                     /locus_tag="ACOIAS_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGZ2701471.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            20116..21177
                     /locus_tag="ACOIAS_001760"
     CDS             20116..21177
                     /locus_tag="ACOIAS_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HGZ2701472.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            21187..22080
                     /gene="mreC"
                     /locus_tag="ACOIAS_001761"
     CDS             21187..22080
                     /gene="mreC"
                     /locus_tag="ACOIAS_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGZ2701473.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            22095..22583
                     /gene="mreD"
                     /locus_tag="ACOIAS_001762"
     CDS             22095..22583
                     /gene="mreD"
                     /locus_tag="ACOIAS_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGZ2701474.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            22595..25573
                     /locus_tag="ACOIAS_001763"
     CDS             22595..25573
                     /locus_tag="ACOIAS_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HGZ2701475.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            25671..26354
                     /gene="minC"
                     /locus_tag="ACOIAS_001764"
     CDS             25671..26354
                     /gene="minC"
                     /locus_tag="ACOIAS_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGZ2701476.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            26378..27175
                     /gene="minD"
                     /locus_tag="ACOIAS_001765"
     CDS             26378..27175
                     /gene="minD"
                     /locus_tag="ACOIAS_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGZ2701477.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            27193..27477
                     /gene="minE"
                     /locus_tag="ACOIAS_001766"
     CDS             27193..27477
                     /gene="minE"
                     /locus_tag="ACOIAS_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGZ2701478.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            27562..28692
                     /gene="rodA"
                     /locus_tag="ACOIAS_001767"
     CDS             27562..28692
                     /gene="rodA"
                     /locus_tag="ACOIAS_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HGZ2701479.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            28733..29146
                     /locus_tag="ACOIAS_001768"
     CDS             28733..29146
                     /locus_tag="ACOIAS_001768"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGZ2701480.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
BASE COUNT        11589 a         3158 c         5680 g         8931 t
ORIGIN      
        1 ttgtcattat agaataactt ggctgtttca aaaatgtgaa atcattttag aaacagctta
       61 tttttttata aaaataaaga aggcaacggg aagccttctt tcgtgtaaaa aaagtttaat
      121 tttttgttaa gatttgtttg ttattttata ttacctttat aatatgtttt tatttggtgg
      181 ttggcaatgg attttgcaca actggtcatt attaatgtaa aagtggaata ttattaataa
      241 ataataatta caaaattgta atctttattt attaaataaa tgagtaaaat aatatattaa
      301 tgggtatata attaagacaa tactagtttg gggaggataa aattatggat tataaaacaa
      361 tcatattaaa tagaaagtct gttagagaat ataagaacac agatattaaa gaagaatatc
      421 ttagtgaaat taagaaatac tcagaatctt gtaagaaact agttccagaa atagatgttg
      481 atgtaagaat tatgaataga agtgaagttt ttaataattt agataaagta gcagggtaca
      541 aaggaaatat gatagattct ccaagttata ttataatatt atctgacata aaagataatt
      601 acattgaaaa ttcagggtac ataggagaaa gtataatctt taaagtaaca gaacttggaa
      661 ttggttcttg ttgggttact tttgaagatg gaaaaaaggt aaaagaaaaa cttggaatta
      721 attcagataa agaggtaaca ggaattatag ctattggata tggagaaaat gtaagtaatg
      781 ctaaagtatt aaatgctaca aaaactgggc aaaattattc aaaatctgat atgcaagttg
      841 taacagataa ggggtctact agactaggtg tagaagatgt tgtttatatg aacgaatggg
      901 gaaatgttgc aagtatagaa atcttagaag aaagagcact tcttgatgca tttcactatg
      961 caagattagc tccatctaca ttaaatagac aaccatggag atttatagtt gatggaggca
     1021 ctgtagtttt agctgttaga aaagatggtc atactaattt atatgaagaa aaaatagaca
     1081 ttggtatagt tatgctttat tttgcaacaa taataagtgc aactatgttt gatttaaaat
     1141 ggaatttagg tactccagac aaagattata aagtgccaga agactataag atagtaggat
     1201 attgtaatat ataagatagt gtaatactta atttaactac acttaaatta atttttataa
     1261 aaagttcaat aacattttac aatataatgt tattgaactt ttttattgta taatacaatt
     1321 tttatattta ttgtattgat tgaaaaatgc ttataaattt aaatgcttac aattttgtgc
     1381 atgaatagag aggtatatta ttttattaaa ctgtataatt tgcttggagg taatatcaaa
     1441 tggaatggat aaataaatta aatcaagcta ttacttatat tgaagaaaat ctcgaaagtg
     1501 gagtggatta tgcagaagca gcaaaaattg catgttgttc aacctttcat tttcaaagaa
     1561 tgttttcata tattgcagaa gtaccattat ctgaatatat tagacgaaga cgtatgacta
     1621 aggctgcatt tgaacttcag aacagtaaca tcaaaatttt agaactatct gagaagtatg
     1681 gttacgattc accaacatct tttaatagag cttttcaaaa tatacataac atttctccat
     1741 ctgcagctag agcaaagggt gttgttttaa aagcatatcc taagatgaca ttaagcattt
     1801 atgtaaaagg aaatatagaa atgcaatatc gtattgtaga aaagaaaggg tttcgtattg
     1861 ttggtataaa agaaagtatg aacatgattg tagaagagtg ctttgagaaa gttcctaagt
     1921 tgtgggctaa atgcataaaa aatggaacaa ttgataaact ttcagagtta ataaataatg
     1981 aaccttgtgg attgcttgga gttagtgtgt gtacaaatag taagggttta gattattata
     2041 ttgctgctcc aacagataag cctgtattag aagatactca tgaatactta ataccatcag
     2101 gcacatgggc agtatttgaa tgtgtttctc caatgccaaa tgctctggca atacaggaat
     2161 tgcaaaaaag aattattact gagtggttac caagttctgg gtatgtgtat gctaatttac
     2221 cagatattga actttatccc aatggcgata tcaatgctcc tgattatact acagaagttt
     2281 ggattccaat tcagaaacca agttaaaatt aataaataat atggaattaa taaataacat
     2341 taaaattaat aaacaacatg aaattctaaa attacaggct gaaaatagtc tgttattttt
     2401 ttgtaaatat ggaaagtaag ctttacataa atggaatata gttgtataat tatggaaagt
     2461 agactttccg tagttgagat attaaagggg gatagatgtg aaaaatagat tagaagaaat
     2521 aagaaaagaa agaggcataa aacaagaaga acttgcttct attttgcaag tatcaagaca
     2581 aactataggt tcattagaaa atggtaggta taatccttct atcatcttag cctttaagat
     2641 agctagatac tttgatatga gtattgaaga aatatttatt tatgaggagg agtaaaaatg
     2701 aaaaagaata tgttatatgt tggaattata tattttattt gtggaatttt acttgtttta
     2761 ttggctacat ttactgagtt ttcatttgag gcttttattt ggggattatc tggagctgct
     2821 ttaggtccag gtgtatgtat gattcttcaa tatatgtatt ggagtaaacc tgaaagagca
     2881 gtagattatg aagaaaagat aaaaaatcaa aggatagaga tgaatgatga aagaagaatt
     2941 atgttacgtg ataaagctgg acgtattact aatcaaatca tgtgctatgt tcttgtaatt
     3001 cttatattta ttgtttctat attgagtgta ttttctgtaa tggtaataag caagtgggtt
     3061 ttagttgttc ttggattgct tattttattt caatttattt gtagtgttgt tgtatacaat
     3121 aaattagata aaaggttgta ataagttatg atataaaata gaaaattata taaaagtaaa
     3181 ttagttttta aattaatgct agaagataaa tttatataat tatataaatt ttaaaaagac
     3241 acagtaattt caaaaaataa cttaaaattt aaatcaaaaa taaaaaaact cctaatctat
     3301 tgaataaata gctttaggag tttttaatgg tgcccgaggc cggactcgaa ccggcacgga
     3361 gtattagtcc ggtggatttt gagtccactg cgtctaccaa ttccaccact cgggcatgtc
     3421 atgtatatta ttatagcaaa aaaatatatt gtttgcaaca tatttaattt taatatacta
     3481 ttattattat gggaatatta aataacacta tattggtata taatatatat gttacaaaaa
     3541 tagataattt ttaggagtga tataatttgg aaaaaaaatt aattataata gatggaaatt
     3601 caataataaa tagagctttt tatgctcttc cagaaatgaa taataaagaa gggttaaaga
     3661 ccaatgctat atatgggttt actacaatgc tatttaaaat gatagatata tataaaccaa
     3721 cacatataag tgtagctttt gacagaaaag cacctacatt taggcattta gaatataaag
     3781 attataaggc tggaagaaag ggtatgccag atgaattggc agaacaatta cagccattaa
     3841 aagacctttt agataaattt agaataaata gactagaaat agatggttat gaggcagatg
     3901 atattatagg tactgtttct aaaaaagcag aagaaaatgg atataaggta tacatagtaa
     3961 ctggagataa agatgctatt caacttgctt cagataatac aactaccctt ataacgaaaa
     4021 aaggggttgg agaagtagaa gaatacaact ttaattctgt aattgaaaaa tatgagatga
     4081 ctccaacaca atttatcgat ttaaagggtc ttatgggaga caagtctgat aatatacctg
     4141 gagtacctgg aataggtgaa aaaacaggta taaagcttat aaaagagttt tctagtatag
     4201 agaatctaat agagaataca gataagctta aaggtagtgt aaaaaagaaa atagaggaaa
     4261 acaaagaaat agctattcaa agtaaaagac ttgcaacaat tataagagat gtgccaatag
     4321 aagtggattt agatagtatg atttttggcg attatgataa agatgaactt ttagataaat
     4381 ttagatactt tggatttaca agtctcttat caagattagt tgatttggtc gattcagatg
     4441 acacagaaca cgtagaagaa aaaatagaaa tattaaaatt aaaagatata gaaaaattta
     4501 tagatgaagt aaacaaaaaa ggacaagtta ttttaaaaac tgttagagga caaggtaata
     4561 tacttgaaaa aaatataata tatattttta taagtgtaga tggagaaaaa atatattatg
     4621 tagattcaaa tgaagtgtca aagttagata gtatattttc aaataatgaa attaaaaagt
     4681 atggatataa tttaaaagat gattatatat ctttaaaacc atataatata aacttagaaa
     4741 atatgtattt tgatataaca atagctgagt acttaataga ttcaacttca tctacctcat
     4801 atgattgtag tgcaatagct atgaagtatt tatcaaaaaa ggttaaatca aaagaagaat
     4861 tattaggaaa gggtgttaaa tctaaaaact atgaggattt agaatttgag gatttagcag
     4921 agtatatggg tcaaataata tgcacagtta aagaaacagt tcctatgatg gaaagaagtc
     4981 taatggatat gagtatggat ggtctttttt atcatgtaga gatgccttta gtagaagtct
     5041 taggtcatat ggagtatgaa ggtataaaag tagacaaaag tatattggat gaattgagtg
     5101 ttgaatttaa agaaataata gctacattag aaaaggaaat ttatgaatta tcaggagagc
     5161 cattcaatat aaattcacca aaacaattgg gtgttatatt atttgaaaaa ttagagcttc
     5221 caataattaa aaaaactaaa actggatatt caactaatgc agatgtttta gaaaaattaa
     5281 gagataagca tccaataata gataagataa ctgagtatag acaaatagta aagttaaatt
     5341 ctacttacgt tgaaggactt ttaaatataa taaatccaga aagtaataga atacattcat
     5401 catttaatca aactataacg actacaggaa gaatttcatc tacagaacca aatcttcaaa
     5461 atatacctat aaaaatggaa atgggtagaa aaataagaaa agtgtttatt gcagatgata
     5521 actgtaagtt ggttgatgcc gattactcac aagtagagct aagagttctt gcacatatga
     5581 gtcaggatga aaatatgata gaagcattta aacatcatga agatattcat accaaaacag
     5641 cttcacaagt ttttaatgtt cctatggaag atgttactag tgaattgaga ggtgcagcta
     5701 aagctgtaaa ctttggtatt atatatggta aaagtgactt tggtctagct gatgatttaa
     5761 atatacctgt tccaaaggcg aaagaatata tcgaaagtta ttttgctaaa taccataaaa
     5821 taaaagagtt tatggacaca acagtagaaa aggctagtga ggatggatat gctgtaacaa
     5881 ttttaaacag aagaagatat ataccagaaa taaagtcaag caattttatg gaaagaaata
     5941 gaggaaagag atttgcaatg aatgcaccaa tacaaggaag tgcagcagat ataataaaaa
     6001 ttgctatgat aaatgttcat aaaaaattgg aagagaacaa tttgaaatca aaacttatac
     6061 tacaagttca cgacgagttg atagttgaag caattgatga tgaaatagat atagttaaga
     6121 aaatagtaaa agaagaaatg gaaaatgctg taaatatgga tgttcactta gatgtagatt
     6181 taaatgtagg tagttcttgg tatgagacaa agtaggtgat tttatgttga tattaggact
     6241 tacaggagga ataggctgtg gaaaaagcag cctatcaaat atatttagaa atcttaacat
     6301 tcctatagtg gatgctgata ttatatccag gaagatattt gaggataagt tgttattgga
     6361 aaaagtattt gtccattttg ggcaaagtat aaaaaatgat gatggaactc taaatagaaa
     6421 agctcttggg aaaatagttt ttagtgatga agaaaaactt aaggaattaa ataacttgac
     6481 tcatccacga ataagagaaa aaataataag tgaaatagaa aaattgagaa aaaaaggcga
     6541 aaatatagta gtgttagatg cggcaatatt ggtagaaagt ggttttttag atatggttga
     6601 caagctatta gtagttactt gtaaacaaga agtacaaatt agtagaatac aaaagagaga
     6661 taattgcagt gagcaggaag cacttagtag gataaattca cagatgagtc aggaagaaaa
     6721 atccaaatat ggtgattata taatagataa ttcgggaaca ataactgaat tagaaagtaa
     6781 agcacataaa tttattgagt acatgaagga gaattggcgt gaatagtaag aaagtattga
     6841 ttctatctat ttttataatc ttatttgggg cactattaat ggaaagcaaa gtaatacata
     6901 aatttttata tcctaaaaaa tattcagagt atgtagaaaa gtattcgaaa gaatttaatt
     6961 tagatgaaaa tatagtttac agtgttatta aagccgaaag taagtttaat agttctgctg
     7021 tttcaaaaaa ggaagcaaaa ggattaatgc aaatattaga cataactaga gattggggag
     7081 cagaggaact aaatttaaaa aatgtggata ttttcgaccc agagactaat ataagacttg
     7141 gctgttggta tttaagtaag ttatacaaag aatttggtaa attagattta gtgatagctg
     7201 catataatgg tggttcaggt aatgtgaaaa aatggttaga aaataatgaa tatagtaaag
     7261 atggcgaaaa tctacatgat atacctttta agcaaacttc aaaatatgta gaaaaagtaa
     7321 aaaataatta cgaacattat aataagatat atggcaagaa aggaaaaaac taatgaagag
     7381 aataaaagtc ttaacagtcg ttttagctat aactcttatg gtagctggtt gtagtaatac
     7441 taaaacaaaa caaagtagta gtgattcaag tttacaaagt caaagttcta tagatgctaa
     7501 gtatataaac ttaacaatgg taactccaaa aactataaat cctataacta atactgataa
     7561 gtctgttggt tatataatga atttagttta tgatagttta tttactatag atgagaacta
     7621 caatgtaatt cctcaattag ttaaggagta taatatagct caggatggaa tgagtataga
     7681 tataaagcta aaagatgcaa agtggcatga tggaaaaaat attacttcaa atgatgtaaa
     7741 ctatactatt ggtttaattc aaaaaagtgc agatagtcca tataatgaat ttactaaaaa
     7801 tatagcttct gtgaatatta aatcagataa agattttaca ataaaattta aagctagata
     7861 tgctttttca attgatagtt taatttttcc aattgtatct caaaatcatt tagattcaaa
     7921 agatgtaaat gacaagaata ataatatgat aggaaatggg aaatataaaa tagaatcata
     7981 tacagaaaga gaaggaatgg ttttatctgt aaacaaagat tactatgaag aagttccaaa
     8041 aaccatgaag aacataaaag taggtatggt gcctaatgaa gatgcaagga catctatggt
     8101 tatggctctt gatagtgata taaccaatgt tacattaaat gatttaagta agtttcaaga
     8161 aaaagagttt aatataacaa aatatcaagg aagagattat gagtgtgtat tatttaatta
     8221 taataatcct tttttcaaag atgttaattt tagaaaagca attgcacact ctatagataa
     8281 agatagaatt attagtgaag gttatatgga tgatgctact ccagttaatt ttccacttaa
     8341 ttcaaaatca aaatactata atagtgaaat gaaggattta gagtttaata aagataaggc
     8401 gatagagtgt cttgctaagg tagaatatgc aaatgtaaac tcagtaaatc aaaatgacaa
     8461 taaagtaaaa aatcagaaaa aatcagctaa aaagaactca aagaagctta cgccagaaga
     8521 agaagcaaaa gctaaaaaag cagaagaaga tagaatcaaa aaagaagctg aagagaaaaa
     8581 aaatagagaa aaagaggaag taaagaaaag tctttctgaa atgaacttaa agataatagt
     8641 aaacagagat aatagtgaga gaataaaaac tgcaaacata ataaatgaaa acttaaaagc
     8701 tataggaata aatagtacag taaatcagct gtcagataag gatatggaaa atgctcttaa
     8761 ttctaaaaat tatgatttag cattagttgg atggaaacta tcaattatac cagatgcaag
     8821 tagtattata gcgagtagtg gatatactga tgataagtta aatggatata tgtctgcact
     8881 tacaagttct acgtctgaaa ttgaaacaaa aaaagcatac aaagatgtac agacatatat
     8941 aaaagataat gcaacattta taagtttagc tgtgagaaac aactatattg taagtaatag
     9001 tagattaaaa ggtaaaatta ctccaaatga ttttgatgta tatgagggaa tatctaattt
     9061 agatataaaa tcgaatgaat caaattaaat gattaatttc tatttaaaaa gtataaacta
     9121 tatgatagaa tacccttata gggtataagg aggtaattaa atgaagaaga aactattagt
     9181 agaaggtatg agttgtggtc attgtgtaaa ccatcttaaa actgcattaa cagaagatat
     9241 agatggtatt gaagttttag atgtagattt agaaaataag tgtgcatctg tagatatgaa
     9301 agatgatgta tctattgaaa aattaaaaga agtgatagca gaattaggat ttgaactaaa
     9361 gggaattgaa tagaaattga taaactaaaa aaagacctct attgggggag taatagaggt
     9421 ctaaaaatgg gggagattga gtatgtttga ttttacatta ctattatctc cccttttaaa
     9481 ttaatatata cataaaaagt aaaaaaattt tgtaatgact cacatttctt gagctttttg
     9541 aatatactat aaatagctaa gggagggagt atcatggaat ctaggggata taaagaggat
     9601 ttaaaaaaaa tgatacttga tatggcccac aaagaacttg agagttatgt atcacaaaaa
     9661 ggagcaaaaa aatactttga aggtaacttg gataagatta ttaataataa tataaaaaaa
     9721 ttcaatcaaa gtattggtct taatagagta tatttaaaaa tgttaaaaaa gtgtgcatat
     9781 caagaaagtg taacttcaat atcaagaact ataaagaaag aagcattatt taaatcaaaa
     9841 aaagagctat ttgctttttc agagtactta aaacttgata ttaacaagcg actttcatat
     9901 aatcaaattt taagaaaaat atcaagatat atatactaca atagaagttc atatgctcaa
     9961 aaatacgttg ttttcaaaag aggagatgaa gaatatcttt tagaaccaga gaaaataaaa
    10021 gatgaattaa tatcaagcta taggtcaaag actagagaag atatgaggtc aatagcaaaa
    10081 cttttggata tagaagtaga agatgattat aatgctgaag atattagaaa aaaagtaata
    10141 aattatatta ttaaagaaaa acttaaaagt tagagtttta aagaataagt tctgccaatt
    10201 tgcatgatta agtataactt gggtataaat tttttatgca tttgaaagag cttattcttt
    10261 ttttgacata ttcttttaaa taatttacca tgatttggta attggtcatc atcattcaaa
    10321 tttttaactt aatatgattt aaataaattt ataataatga tataatataa aaaaaataaa
    10381 aattggagag tgatttcaaa atgaagttta atttttgtca taacaatttc aatgttacta
    10441 atttagaaaa aagtttagat ttttataaaa aagctttagg gcttaaagag gtaaagcgca
    10501 aggaagcaga agatggaagt tttatattag tttatttagg agatggcatt actagtcata
    10561 cattagaatt aacttggctt agagattggg atagaccata taatttagga gacaatgaat
    10621 ttcatctggc tttagaagtt gaggagtttg atgaggcaaa aaaacttcat aaagatttag
    10681 attgcatatg ttttgaaaat gaaagtatgg gaatttattt tatagcggac ccagataatt
    10741 attggattga aatacttcct aaaaatcact aattttaagg atatgtttat aatttcttta
    10801 caattaataa gaaaataagt tatcattaaa ttgtagggac taaaaatgga taataatgtt
    10861 atgaatttat gactataaaa atttgttggg gggagtgata gtagtgaaaa aagctatagc
    10921 agctttagga attggggcag tagcagtatc tgttagctcc ataaatgcaa gtgctcttga
    10981 aaagggaaca gtaacagcaa gtgccttgaa tataagaagt ggaccaagtt ctgattgcga
    11041 taaggtagca aaattataca agggaaaaac tgtagagatt ttagaaaagt ctaatggatg
    11101 gtataaagta agggtatcga gttctgttgt tggatgggga agtgcaaaat acatatcaac
    11161 aagtggttcg tctgaaggta catcaagtca aaataatcca acttcaagtg ggacaactat
    11221 tagtggaaat ggtaaagtaa atgtgagttc tagactgaat gtaagaagtg gtgcaggtac
    11281 taactattca ttagttggta aagcaaataa tggagatgta gtaaagttac tagaacaaag
    11341 caatggatgg tataaaataa aactatcaaa tggagttact ggatgggcta gtagccaata
    11401 tatttctaaa acatcagaag atgttggaac gaacaattct agtaattcaa atagtactaa
    11461 taattctgat aaaaaaccat ctagcgaaga atcaattgaa ggcaagaatg gtaaggtaac
    11521 atctgctgtg agtttaaatg taagaagtgg tcctgggaca agctattcta taataggaaa
    11581 attaaatggt ggagatgttg ttgaattaaa agctaaaagt aatggttggt ataaggtaaa
    11641 gttatcgagt ggaacaattg gatgggtgag tgccagttat atttctgaga ctaatgagga
    11701 tacaaaagaa aaaccaaatt catcatctaa ccaaaattct caatctaata gtaactcaaa
    11761 gccatcattt actggaaatt cagataaatc tacagcaaaa ggttcaacaa tcgtagattt
    11821 tgcatatact cttattggaa taccatatca atggggagct tcaggacctg ataaatttga
    11881 ctgttctgga tttacacagt atgtttttaa acattctgta ggcgtttcta ttcctagagt
    11941 ttctagagaa caggctaatt ttggaagtgc aatttctatg ggaaattatg caccaggaga
    12001 tttagtttat tttgacactg atggtgatgg aacaactaat catgtaggta tatacgtagg
    12061 aaatagtaaa tttatccatt gtagtggtac tcagactaat cctaataagg ttaaagtgga
    12121 taatttaact agttcatatt ggtctaaagt attacttggt gcaagaagat ttgtttagta
    12181 agtacaattt cttattaaaa aattagaaga aataattatt tatattcata aattgtgact
    12241 cttaaaaata tactttatta ataatataat tttgggaggg acaaactaat gaatagaaga
    12301 aaaacgatat acagaggatt taataataat agacataaga ttgatttacg taaatatgtt
    12361 ataacgattg cttgcttgtg tctaattggc tattattctt atacaaaaat aaaagatagc
    12421 aagatattgg agtatgtatc ggctaaaata ccatttctaa ataattcatc agatataact
    12481 tataaagata tatctgatga attaaattca ataaaaaaag ggaaaaaatc taaatcacaa
    12541 accaattcag atgataaaca agaaactaat ccagaaaaag cggtaaataa cactaaagag
    12601 ccagaagaag tcaaattagc tactatagaa ggctgggata tgtatactat tcaagttgct
    12661 gcaatagata ataatgatga tttgaaaaaa atacagacat cattagttaa taatgacata
    12721 cctttttcag ttatggagaa ggatggagta aaaaaaatac agacctattc ctcttttgat
    12781 gaaaatgatg ttagaaagca gataagcagt gtaagaaagg tgtttccaga tgcattttta
    12841 tcacacttag atgcacctat gttgtcttta gagtatacaa gtaactacgc ttatatagag
    12901 agtatatcta aagaattaaa taaattaata acaaacttta aagaagaatc aagtttttgg
    12961 tcgaatgctg aaaataatgt agatatggaa aaatataata ctattttgac aaatagaaag
    13021 gcaatatcac aaaatatatc aaaagaagct gaaaagattg attattctga aatgcgttta
    13081 tttaaagata atttaataga gtatgtaaag aatgtgaatg aaaaaattga tacagcttca
    13141 aaagcagcaa atgaagaaaa atatagtgta agtaaaagtc tgcttttatc atccatgcag
    13201 gagtactcta tgtttataaa ttctataaaa taaaatacag aaaatttgga ctttagaaag
    13261 ctatatgaaa aggattgttt aattaagcaa tccttttcct caatttgact taatatagag
    13321 gtgttgattt ataaaataaa ttataaaaat acggatattt ttttaacaat tgttaagatt
    13381 ttttaatata tatagtatac ttatagaagg cagaaaaaat gtttttttat agataagggg
    13441 gtaaaacaat gaaactctta acttttaagg gagggataca tcctccatat agaaaagagt
    13501 actctaacac aaaggcactt gaaaaagctc aagctccaaa gatagtttac attcctcttc
    13561 agcaacatat aggggcacca gctaaaccta ttgttgaagt tggtgatgaa gtaaagtttg
    13621 ggcaaaaaat aggtgaacaa caaggttttg tttcatgtaa cgttcattct tcagtatcag
    13681 gtaaagttat tgctattgaa caacatgaag taccaggagg ttcagctcaa tgtgtagtta
    13741 tcgaaaatga ctttaaggaa gaattacatg agagtgttca accaaaaggt cagttagagg
    13801 atttgagcaa agaagatata gtaggtatca taaaagaggc aggaatagta ggtatgggtg
    13861 gagctacatt ccctaaccat gtaaaagtat ctcctccacc agatagcaag gcagaagtag
    13921 ttatattaaa tggtgcagaa tgtgaaccat acttaactgc agaccacaga ttaatggttg
    13981 aaaatccaga agatgttgtg tttggtttaa gagcattaat gaaggtatta gatgtcaaaa
    14041 aaggatttat aggaattgaa acgaataaac cagatgctat agaagcaatt caaaatgtag
    14101 ctaaagacta tagcgaaatc gaagttgttg gccttcaagt gaaatatcca caaggagcag
    14161 agaaacaact tatttatgcg tgtacaggca aagaagttcc atctggtgga ttaccaatag
    14221 cagcaggtgc agttgttgac aacgttgcca cagcagcaca gatagctaag tctattaaaa
    14281 caggtatgcc tttggttgaa agaatcacta caataacagg tagttgcatc aaagagccaa
    14341 agaacctaat aacaaaagtt ggaacattag tttcagaaat aatagaacaa tgtggtggat
    14401 ttaaagaaga taagaagatt ggtaaggtta taatgggtgg acctatgatg ggaatagctc
    14461 aatatactac tgaaatagcg actaacaaag gttcttcagg aattttatgt ttggatgagg
    14521 aagaatcacg tactccagat attcaaaact gtttaagatg tggaagatgt acagatgtat
    14581 gtccatcatt cttacaacca cttttcataa gtgcatattc tttaaaagat gactatgaca
    14641 cagctgaata tcatagagca atggattgta tagaatgtgg gtcttgctca tttatttgtc
    14701 cagcaagaag accattactt caatctataa gatcagcaaa gagagaaata ggagcaaaga
    14761 gaagaaagca agctgctcag aaataaagaa agttaagggg gaaaaacgat ggaaaataag
    14821 ttgatagtat catcttctcc tcatgtgaga agtaatgaag atacttcata tataatgaaa
    14881 caagttatta tagcactcct tccagcagca gtagcaggag tatacttctt tagacttaat
    14941 gctttgagtg ctatgttttt ttgtatactt ggtacagtag gtactgaatt tttatatcaa
    15001 aaatttacga agcaaaaaag tactatagga gatttttcag ctgttgtaac aggattatta
    15061 ttagcattta acgtaccagc atcacttcct tggtggatgt gtctagtagg aggaatattt
    15121 gcaatattag tagttaaaat ggtatttggt ggaattggat gtaactttgt caatccagca
    15181 cttgctgcaa gagcattttt attagcatca ttcccagtag caatgactgc ttggactcaa
    15241 ccaggtgtta actggatagg taaaaattta gatgcagtta ctacagcaac tccattaagc
    15301 tttttgaaaa atggagcagc aggactggca gatatttcta gcaatggaat tagtcttgct
    15361 gacatgatga tagggaatat tggtggatgt ataggtgaga catcagcaat attattatta
    15421 ttaggtggag tatacctaat gtataaaggc ataataaatt atgttatacc agtattttac
    15481 atagcaactg tatttatatt aacattcctt ttaggtggat ttaatataac tttcgcaata
    15541 tatcaactgt ttgctggagg acttatgtta ggtggattct tcatgcttac ggattataca
    15601 acttcgccta tgactaaaaa aggtcaaata atatacgctg tattagctgg tcttataaca
    15661 actgttataa gaatgtatgg tggatatcca gaaggtgtat cttactcaat actattagtg
    15721 aactgtcttg ctccacttat agataagttt gttagaaata gagtatttgg ggaggtggct
    15781 aaataatgaa cagtatggtg agattaggtg gtactttact tgctataagt gcaatagctg
    15841 cattagctct tggagctact aatcaggtta cagctccagt aatagaacaa agaaatatac
    15901 aagcaaataa tgaacttaga aaagcagtcc ttccagaagc aaaagaattt aaagaaatga
    15961 aggaagatac ttacaaaggc cttggagatg gtcttatagc tgaagtgtat gaaggtttag
    16021 atggttctga agtggttgga tatactttaa aagctaaacc aagtggatat ggtggagaaa
    16081 ttgaagttat ggtaggtata tcatctgaag gtcaagttac aggtgttgac attggtaata
    16141 tgtcagaaac agcaggtctt ggagcaaaag ctaaggatga tgcatttaag ggtcaataca
    16201 aaggaaaaac tgctgagcct cttgaagtag caaaaggaag tacaacagca gataatcaaa
    16261 tattagcaat atcaggtgca actattacat ctacagcagt tactacagga gtaaatgctg
    16321 ctatagatgt tttcaatagt gctcttaata aataaggagg gttaattatg aatttagcta
    16381 aagtatttaa aaatgggcta atagatgaga acccaacatt tgttcaagtt ataggtatgt
    16441 gtccaacact agccgttaca acttcagcaa taaatggaat tggtatgggt ctttcaacag
    16501 cagcagtttt aatatgtgca aatttagtta tatctttaat aagaaaaatt acaccagata
    16561 agataagaat acctatattt atagtagtta tagctacatt tgttacaata gtaggtatgc
    16621 ttttaaaagc atatgttcct gctcttgata aggctcttgg tatatatata ccattaatag
    16681 ttgttaactg tttaatatta gctcgtgcag aaagttttgc tttcaaaaca ggagctatgc
    16741 cttcaatagt tgatggtgta ggtcaaggtc ttggatttac tgttgcactt acaataatag
    16801 gtgcagtaag agaattactt ggaaatggta gtttatttgg aatgacatta tttggagcat
    16861 ctttccaacc agtattaata tttatattac caccaggtgc attcttaaca ttaggattct
    16921 tatttgcagg atttaataaa ttaagaagta aaaaagcata gtggaggtgt aataaatgaa
    16981 tttaatactt ttatttttaa gtatcgttct tgttaataac gttataacat ctcaattctt
    17041 gggtatatgt cctttcttgg gagtttctaa gaaggttgat acagcagtag ggatgggagt
    17101 tgcagttacg ttcgttttaa ctctagcttc aattataact tattttatac agattttatt
    17161 agtaaaaact ggaactggat tcttacaaac aatagctttt atattggtta tagcttctat
    17221 agttcagttc gttgaaatgg taattcaaaa gatgagtcca tctttatatc aagcattagg
    17281 agtttattta cctcttataa ctacaaactg tgcagttctt ggtatagctc tagttaatgt
    17341 tcaaaaaagt tataatttag tagaaacaat aataaacgga tttggagccg gagctggatt
    17401 tactttagca atagtaatat tcgcaggtat aagagaaaga ctagaattag ctgatatacc
    17461 agaagcattt aaaggtttcc ctataacgct tatatcagca ggtttaatgt caatagcttt
    17521 cttaggattt acaggactta taaagctata aaaaagaggg gtgaaaatgg tgatacttac
    17581 agctgtatta gttttaggaa ttatgggctt aatcttcggg atagtactag attttgcatc
    17641 taaaaagttt gctgtagaag tagacgagag agtggaagct atactaggag tacttccagg
    17701 agccaactgt ggaggttgtg gatttccagg ttgtggaggt cttgcaaatg cgatagttga
    17761 aggcaatgca ccagttaatg gttgccctgt aggtggagca gatgttggtg caaaagttgg
    17821 agaaataatg ggaataagtg ctgaagcagg tgagaaacaa gtagcaaaag ttatttgtaa
    17881 aggaacttgt tcaagtgcaa aagataaata tgagtatgaa ggaatatcag attgtagagc
    17941 agcaaatgtt ttaaactcag gtgctaagat gtgtaaattt ggatgtttag gtcttggaac
    18001 atgtaaagat gcttgtaaat ttgatgctat ttctatagtt gatggaatag cagtaataga
    18061 tgaagaaaag tgtgtaaatt gtggaaaatg taaagaagtt tgtcctaagg gtataataat
    18121 cacaaaacca gaaagtcaag aagtagttgt agagtgtaat agcaaagagt ttggtaaagc
    18181 tgttaaagaa aaatgtactg ctggatgtat tggctgtgga atgtgtgtaa aagcttgtaa
    18241 atttgatgca ataatatttg aagacaaaat agctaagata gaccctaata agtgtgtagg
    18301 ttgtatgcaa tgtgtagcaa aatgccctac aaaagtaata tcaggtgaca taactaagaa
    18361 aaagaaagtt actattgacc aagaactttg tgttggatgt acagtgtgca aaaaacaatg
    18421 taagtttgat gcaatagagg gagaattaaa agaaaaacat aaggtagatg cagataaatg
    18481 tgtaggatgc catttatgta tggaaaaatg tccaaagaaa gctattaaaa ttttgtaata
    18541 tggacaaaat aaggatatgg aataatttcc atatctctaa tgagtacata aaacagaggt
    18601 atggaataat ttccatacct ctattttatg tatatttatt ctaatggtaa taaatataac
    18661 aatagtgtaa caaaaaaggt tcgacatttc tgttgaattt tttacaatat agtgatagac
    18721 taaataggta caaattacgt acaaatgagg tgaataaatg aatataatct tggcatcagc
    18781 ctctccaaga aggaaagaaa ttctagagaa tacgaatgta agatttgata taattaaaaa
    18841 tgaaattgat gagattatat tggaaggtga aacaccaaaa catttagtta tgagattagc
    18901 ttttgaaaaa agtatgtctg tagcatctga acataatgaa gatgttgtta ttggagctga
    18961 tacagttgtt gttttagaca atgctatttt aggaaaacca aaagatgagt cttgtgcaag
    19021 agatatgtta aaaagactat ctggaagaga gcatcaagtc ataacaggta ttagcttaat
    19081 aaacttgtgc gaagataaaa aggtaattga ctatgtaatc agcaatgtga aatttaaaac
    19141 gttatcagag caggatattg aggattatct gaaaactaat gagtcatttg acaaagcagg
    19201 agcctatggt atacagggtt atggagcttt gttagttgaa gaaattagag gagattattt
    19261 taatatagtt ggtcttccaa tttcaaggtt aggcgaccta ttaaaaaaat attttagtat
    19321 aaatcttttt tatggagtgt gatttatctg aaaaagtcat ttaattcaac cattaaggta
    19381 aaagaaatgg caccagagga aagacccaga gaaaagatgc ttgccaaagg cgtaaaaagc
    19441 ttatcaaatg ctgagttgtt ggctatactt ttaagaactg gcaacaagaa taaaaatgct
    19501 attgaattag ccaactatat aataaataga gatattcagg gtattagaca tttagaagat
    19561 atgacaatag aggagctgtg taatatagat ggaattgggt tatcaaaatc aacccaaatt
    19621 aaagcagctt tagaactagg ctctagagta gctagtttta aacctataaa gtataaaata
    19681 aggaatcctt gggatataca gaggtattat atggatagcc taaggtattt aaaaaaagag
    19741 gtttttaaag cggttctttt aaacacaaaa aatgagataa tatctgatgt agatgtatct
    19801 atagggactt taagctcgtc attagtccat ccaagagagg tttttaaaga agctataaga
    19861 agaagtgcaa gtaagataat agtgatgcat aatcaccctt caggaagtgt agaaccatca
    19921 agagaagata aaaatatcac atcaagactt attaaatgtg gagaaataat tggaatagaa
    19981 ataatagacc atataattat tggagatgga ttatacttta gctttaaaga aaatatgata
    20041 atttgacttt atatgtgata aaataatagg agaatgtttt aaacatgatt cttaatagta
    20101 ggaaggagta aaatcatggc taaggaaaaa aagaaagaaa aaaaaggttt ttttagtttt
    20161 aataagatga caaaggatat gggaatagat ttagggactg caaatacatt agtttacata
    20221 aaaggacaag gtattgttgt tagagaacca tcagttgtcg caataagaga tgatagtaaa
    20281 gaggtattag cagtaggtga agaagccaaa aaaatgatag ggagaacacc aggaaatata
    20341 gtagctataa gacctatgaa agatggagta atagctgatt ttgacataac tcaatcaatg
    20401 attagctatt ttatacaaaa agcggctgat aaaaaaggtg ttgtaagtcc aagaatagct
    20461 atatgtgttc cttttggagt tacagaagta gaaaagagag ctatagaaga ggctgcaaga
    20521 caagcaggag ctaaagatgc attccttata gaagagccaa tggcagcggc tataggggct
    20581 ggattaaaag ttgaagaacc agaaggtaat atggtagttg atattggtgg aggtacttca
    20641 gaaatagctg taatatcttt aggaggaatc gttactgcta aatctataag aataggtgga
    20701 gacgaatttg acgagtctat agtggcttat gtaaaaaaag aatataatct tatgataggt
    20761 gaaagaactg ctgaaaatgt aaaaattaac ataggttcta catttaaaga tgatgaagaa
    20821 ataaatatgc aaataagagg tagagattta atctcaggat tacctaaaac aatagaaata
    20881 tgctctacag aagttagaga agcattaaaa gaacctgtaa gttcaatagt agatgcaata
    20941 aaatctacac tagaaagaac tccaccagaa ttagcctcag atataatgga aaacggaata
    21001 atgctaacag gaggaggcgc tttgcttaga ggtcttgata agcttattac acaggaaact
    21061 ggtatgtctg tacaaatagc agaaacacct cttgattgtg ttgcacttgg aacaggtaaa
    21121 tctgttgaag accaagaaat ctttgaaaaa gtattaatga tgaatactaa gaattagaag
    21181 ttggtgatgg ccttgagatt tgacaagaac aaaaatgaga aaaaaaggat taatgttaaa
    21241 gtgatagcaa cgggcgtagt tgctatcact ttaattggaa ttgtgggtat atcaattggt
    21301 aaattttcta gtggtagtcc agtaaattta ggagtagctt cagatgctat tacaagtgta
    21361 ggtaagggaa taaatgatgg attttctttt atcaaaaatg gttttaaaga tgtagcaaat
    21421 tttaaagata attctaaaaa ggtaaaaaaa ttagaagaag aaaatgaaaa acttaaaaaa
    21481 aatatgatag ctttaaatgc caaactagat aaaacagaat cgctagaaga gttaaaaaag
    21541 acactgaact ttgtacaaga agagtataag gcgacaagta tttcagcaag tgttgttggt
    21601 aaaaatgatg gaaactggta tgagagtttt gtcataggtg caggaaaaaa ttcaggtgta
    21661 aaaaaagaaa gtatagttat gaatggtagt ggattagttg gcatagttta tgaggtttcc
    21721 aacaattata gtaaggcaat ttcactattg gattctaagg catctgtaag ctttaaacta
    21781 gcaaaggatg ccaatgctaa aggtacaatt actcaaaata ctactttaga taataaagat
    21841 agttataata gtaaaggata tttgcaagga tatatgtttg actcatcata taatgtaatt
    21901 caaggagata taataacaac atcaggtctt ggattcttcc cagatggtat accaattggc
    21961 gaagttgaaa aagtggttga tgataaagat aagtcattaa aatatgtagt tgtaaaacct
    22021 tatgttgatt ttaagaatat aaatgacgtt gtagtcatag aaccaagaaa tataggataa
    22081 aggggataaa aacaatgaaa aaagttttac tttgtctgtt ggggattgtg cttatgacta
    22141 tagaaaatag tataacaaat tatatagata tttttggcat aagctttaat ttagttctta
    22201 tatatgtaac cataatctct ctttatttag atgaattaga aggtgggatt atagcagcaa
    22261 taatagggct tattaaagac ataaccgttg gtggaatttt tggtgtaaat gcattaattt
    22321 tgtttattat tgcatatgca attggataca tgagagatca gctatataaa ggtagttata
    22381 ttattacttt cgttttaatt tttataggca gtttaattga ttcaattgtc aatataggta
    22441 catctagtat aatttatcaa agctacaaca tatcaacact ttttgtaaaa ggtctactag
    22501 tagcaccaat agtgaatagt ttaattggtt tagtaatata tcgcttatct aaaagagcag
    22561 ttttaaaact taaagaagat tagttggtga tttcatggaa gaaaataaac aagttgatag
    22621 actaaaaata attaagatag tcatgggcgt gatattttta gctattttag taaaaataat
    22681 ctatatgact acatttaaat atgaatatta caatgagtta gctgagaata aaacatataa
    22741 aaagttggca atagaagctc caagaggaga aataaaagat agatatggaa gactgttggc
    22801 aggaaataaa aacttattta ctgttcaagt ttcaggtaac gatattaata agaaagatgc
    22861 aaataagcat tcaagagcaa atgaaatatc attaaaactt ataaatttgc tggagagaaa
    22921 tggtgaagaa tatgtagatg agtttcctat atatgtagaa aatggaaagt actattatac
    22981 atatgacaga gatattaggg aatataaatc agaaaatggt attccaaatg attataatgc
    23041 taaggaaagt ttttattact tggtggacaa acttatatca gctggaatat tatctcaaga
    23101 agataaaaga ttggatgcga caagattaca ggcaaaacta aatgaaaatg gatattatcc
    23161 acctatttta gttagtaaat ggatgttcac agcagaaaga gataagagag attggttggc
    23221 tagttataag ataaaagaaa ctaaactgag tgccaaagaa gcgtttgaaa aagtaagaaa
    23281 tagtgatgca ttagaaattg ataaaagttt gagtgatgaa gatgcaagaa aaataatggt
    23341 cgtaagggat ttgataaaat caaaaggata ttctcaatat aatccagtaa ctattgctaa
    23401 ggatgtggga gaaactacga ttgctcaaat tgaagaaagt gctatggacc ttgttggtgt
    23461 ttcaatagca gtagaaccag taagatatta tcctaatggg tcattagctt ctcatatgtt
    23521 aggatatgta ggaaaaatgc catccactca aatagaatca taccttcaaa aaggttatga
    23581 aactggagat atggtaggtc ttgcaggagt agaaaagagt aatgaaagta gattaagagg
    23641 aactgatggt tataaaatgg ttaaagttga tgcattgggt agaatcagta aagaaattga
    23701 atctaaaaaa cctaaatcag gagatactgt gtatcttaca ctagataaag atttacaaga
    23761 agtttcagat aatgcattaa aacagataat cgaagttgct tctaaaggag gtacttttaa
    23821 aagtaaattt ggggacaaac caattagtgc atatgcagga aaagcacagt cagcagctct
    23881 tatagctatt gatgttaaaa atggagaagt attagcttca tcaagttatc ctaattatga
    23941 cccaaataaa tttgcaaaag gtatttctac agaggattat aaagcacttc aaccaaaaaa
    24001 tccaaatgac ttactagcag gaagtccact tttaaacttg gttacacaag gggaatttca
    24061 gccaggttct tcatttaaga tggtaacttc aatggcagcg ttagaaaatg gtctggatcc
    24121 aaactttact ataaatgacc ctggggttat aatgttaggt aaaaaatctt ttggtgacta
    24181 tgtatggaac catggtagag gaaaccatgg tatgacaaac ttatataaag caatacaaga
    24241 gtcttgtaat atatatatgg caacaattgg tacaggtaaa acatggccag atggaaaaag
    24301 cattggtata gatatgaatg caaataaaat acttgagtat gcaaaattat ttgggcttga
    24361 ccaaaataca gggcttcaag atgaagtaga agaaagagca ggtaaagttc ctagcacaga
    24421 agataaattg aaatcaacac aagctcttct aaagtcaaat ttagaaagag aaatggcaaa
    24481 tgattttgtt gatataacta gagaaaaaaa tcctaaagaa tatgagaaaa gaatcaatga
    24541 gattgtaagc tgggcagcag aaaagaaaac tcctggtaga gttgaaacaa tgaataggct
    24601 aaaaaaattg gatgttaaag aagatagaat tgaagaagta gcagatttag cagtatttag
    24661 ttattttaat tttgcaaaat ggagtactgc agatacattc aacttagcta taggacaagg
    24721 tgaaaatgct tatacaccag ctcaaatatc aagatatgta gctgctatag caaatggagg
    24781 taatttagta gaattatctg tagttgatag agctgtttca agtgattatt catcagttaa
    24841 aattaatgac cagaagaaag tcgaaaaaat accttttaaa aatccagata acttaaaaga
    24901 attaacaaaa ggtatgaaat tggttgcaag acaaggtaca gcgaagagtg cttttgctga
    24961 ttttccaata gatgtagcag caaaaactgg tacagctgag aaaagtggga aaatacctac
    25021 agataacgag tatgaatatt taaaaagtca tatgtcatct tataatgtca acctaaatga
    25081 tgcgattaaa ctagctgata agatgaaagc agaaaaagaa aaagagcttt cattagctaa
    25141 ggaaaaggaa ataaaaaaga aattagaaaa taaagatttg aaagacgaag aaagaaagaa
    25201 attagaggaa gaattagaag atggagtaaa agttagatta gaggacactg ataaggtgaa
    25261 ttcttcatat ctaagaaaag ctataaaaga attaaatcca aaaataacag atgaccaaat
    25321 agatagattt aaacaagatt atggttcttt tacgtggact gttgcttttg ctccagcaga
    25381 tgacccagaa atagctgttg tatgtgtaat tcctcaaggt gattcaagtg tattctcact
    25441 tttaccaact agagaagtta ttggaactta tatgggatta gagccaacta atagtaaaaa
    25501 tgacaacaag acgaatgatg ttaacaattc ttctgacgaa aatataaatt tcgagtcaca
    25561 aattaataga tagctcaatc tatgtataaa ctttagcttt atatagaaaa aataaaaggg
    25621 tttaaagcgt atttgaagaa tataaatgat atatcactct ggaggttgtt atgtctttaa
    25681 gagaaatttg tagtcaagaa ctagtagagt tcaagggtaa taaaagaggg ataatagtaa
    25741 atataaaaag agaagctcca tttgaggaaa tacaggaaaa aataataaac aaattggaag
    25801 catatgttgg cttttttaat ggggctaaaa ttagtaaaat taatagtgat tgtttaactg
    25861 acatggagat tctagaactt aaagaaggaa ttacttctag atttgatgtg gaatttgttg
    25921 aagatcaaaa aattgaagaa aatagtaatt ttccaacaaa atatgtcaat accctaagat
    25981 ctggtgaaaa tattgaattt gaaggtgatg tagttatatt gaatgatatg aaacctggtt
    26041 caaaagtcct ttcaaaatca aatacagttg ttatgggaga tataaatgct ggtgctaagg
    26101 ttgtagcagg aggtaatgtt tttgtaatgg gtaaaataga aggttttgta catgcaggtg
    26161 cagaaggcaa tgaatttgca tatgttgttg ctggaaatct taatcctaaa atattacaaa
    26221 tagcagataa tattgcagaa gctccagatg atgaagaaaa ttatgaaagt gaatcagaaa
    26281 ttagcccaga aatagccttt gtaagtaatg gaagaattgt aatcgaaagc tatttgtcaa
    26341 agttagacaa atagaaagaa aaataggggg tagtattatg agcgaagtta tagttataac
    26401 atctggtaaa ggtggggttg gaaaaactac taccgcagca aacttaggaa ctgcgctaag
    26461 cctagaaaat aaaaaaacag tagttgtgga tgcagatatt ggacttagaa acttagatgt
    26521 agtaatgggt cttgaaaatc gaatagttta tgacatagta gatgtagttg aaggaacttg
    26581 taggcttaag caagctctaa taaaagacaa aagatttgat aatctatatt tattaccagc
    26641 agcacaaact agagataaaa atgctgtctc agttgagcag atgattgacc tgtgtgagaa
    26701 actaaaagaa tctttcgagt atataataat agattgtcca gcaggtattg agcaaggctt
    26761 taaaaatgca gtagctgggg cagatagagc tattgtagtt actaatccag aaatatcagc
    26821 agtaagagat gctgatagaa taataggtct attagaagca aatgaaataa aagagataag
    26881 attagttata aatagaatta gaaatgatat ggttaagcgt ggagacatga tggataaaca
    26941 agatataata gaaatattag caatagattt gttaggtctt gttcctgatg atgaaagcat
    27001 aattatatca acaaataaag gagagccagc tatacttgat tctaagtcac ttgctggtca
    27061 agcatacaaa aatatcgcaa aaaggatact aaatgaagaa gttcctctac ttgatcttga
    27121 agttgaagat ggattctttg gtagacttaa aaaaatgttt agcatggcta agtagcaaag
    27181 gaggatacat ctgtgttaga tttatttaga gttttttcta acgaggctaa aactagtaaa
    27241 tctgttgcta aagagaggtt aaagctagtt ttagttcatg atagagtaga ttgttcacca
    27301 caacttttag agatgataaa aacagacatt ttaaaagtaa tagctaatta tgcagaaata
    27361 gaagatgatg gtcttgaaat aaaaatgtct aaatgcagag gtgagcatga tgacaaacct
    27421 gtatctgcat tagttgctaa tataccacta aagaatataa aagatagatg tatgtaatta
    27481 gtgcttaaaa gggggatatc cttattggta tatgcccctt ttatttaatt atatgtttaa
    27541 agatagaaag acggtgattc tttgaaaaaa atcaatatta attataagtc gacaattaag
    27601 ttgataaaac agttagactg gaaacttata gttacagtat tagctatctt tatttttggg
    27661 cttgttatac ttagtagtgc aacacatgct aattcaacag gttcatataa tcaattgatt
    27721 aaacaaggat tagcttttgt acttgggata ggaatgatta tagtcatatt attttttgac
    27781 tataaccttc taggcaggta ttataaagcg ctttatataa taagtcttat tttgttagcc
    27841 atagttttat tacctggaat tgggacagta aaaggtggag ctaggtcgtg gattaactta
    27901 ggaccacttg acttgcagac atctgaaata gtcaaattaa catttgtact aagttatgca
    27961 aagatacttg aatccaaaaa ggataaatta aatacgttaa aggaagttat gcctgttgta
    28021 gtttattctc ttccatttat cggattattg attgcacaac cagacttggg aacaggaatt
    28081 gtattttgtt gtatgatatt tgcaatgctg ttcacagcag gtcttagctc aaagttgata
    28141 aaaagaggaa taatcatact attagtgtca atgccactga tgtatttaat gatggcagac
    28201 catcaaaagg taagaataga agcttttcta aatccagaag atgtaactct aaaaggaaac
    28261 tatcaagtta tgcaatctct gattgcaatt ggttctggag gcgtaactgg taaaggactt
    28321 tacaatggaa gtcaaaatca agaagatttt ttacctgtac aagatagtga ttttatattt
    28381 gctgttgtag gtgaagaact tggtgtaata ggaatggcag tgttgataat cttatttatg
    28441 atattcctat taaggctttt agcaattgca agagatgcaa aagattttta tggaaccctc
    28501 atagtggttg gtgttatggg aatgtttggt taccagataa tccaaaatat aggtatgacg
    28561 gttgcgttaa taccagtaac tggagtaaca ttaccttttg taagttatgg aggaagttcg
    28621 ttattgacat ctttagcaaa cttaggatta gtactaaatg tgtgtatgag aagaaagaaa
    28681 ataaatttct aaatttaaat acatctaaac ataaattgag gagattagat atatgaatat
    28741 agcattagta gcacatgacc aaatgaaaaa cactatggtc ggattttgta taggttatga
    28801 atcaatttta aaaaagtatg gactatatgc aactggaact acaggaaaaa gaataatgga
    28861 tgaaactgaa ttaaatataa atagattagc atctggtcct ttaggtggag accaacaaat
    28921 aggttcttta atagtaacac aagaaataga tttagttatt tttttaagag acccattgac
    28981 atctcaggct catgaaacag atatacaagc gttaataaga ctttgtgatg tttatcatgt
    29041 tccaatagct acaaatctag cttcagcaga aatatttata aaagctcttg atagaggaga
    29101 actatcatgg agagaagtta gaaaaagcaa aagtcaacgt atttaaaaaa tattaatatt
    29161 acagtctaaa gaaacagata tataagtgtt taatgcccta gtctttctgt tggaataaat
    29221 ctagtacctg tagaaatctt atgaaagatt taaatgatga agagctaaat ttgagattaa
    29281 ataattagga aatagattat agatagagta gtagaattta aactattact ctattttaat
    29341 aatatgaaat agaatcaa
//
LOCUS       DBGJFU010000042        27270 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.042, whole genome shotgun sequence.
ACCESSION   DBGJFU010000042 DBGJFU010000000
VERSION     DBGJFU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.042"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..896)
                     /locus_tag="ACOIAS_001769"
     CDS             complement(<1..896)
                     /locus_tag="ACOIAS_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HGZ2701481.1"
                     /translation="MKLKHIAIIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQMQYDATQHIPLSQA
                     KAGELVFFHSTYNAGSYVTHVG"
     gene            complement(893..3085)
                     /locus_tag="ACOIAS_001770"
     CDS             complement(893..3085)
                     /locus_tag="ACOIAS_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HGZ2701482.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPENEDGKTREEVVKTEIESNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTVSNARTTRNNSAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDIPTQTAYAVHSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPVTASA
                     IPKTNVEKTQEVKRPATTSTSKAGEPVKTNSVKERPLSSGTSDGKIPQSAQTVHSKNV
                     EKVVSHETHQNYKAERTTKAQTVEQAKRTTEHTEKNRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="ACOIAS_001771"
     CDS             complement(3085..5535)
                     /locus_tag="ACOIAS_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2701483.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEHQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRCEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGEILSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            complement(5522..5911)
                     /locus_tag="ACOIAS_001772"
     CDS             complement(5522..5911)
                     /locus_tag="ACOIAS_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2701484.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVSYVPD"
     gene            complement(6029..6532)
                     /locus_tag="ACOIAS_001773"
     CDS             complement(6029..6532)
                     /locus_tag="ACOIAS_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2701485.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            complement(6535..7038)
                     /locus_tag="ACOIAS_001774"
     CDS             complement(6535..7038)
                     /locus_tag="ACOIAS_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGZ2701486.1"
                     /translation="MQETRVLVETKRTTGEETTSYWFDLPIEVAEFEEKLGVSTESQD
                     YRIIEKLLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(6957..7253)
                     /locus_tag="ACOIAS_001775"
     CDS             complement(6957..7253)
                     /locus_tag="ACOIAS_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2701487.1"
                     /translation="MNDIFRDMQAKVGCENISDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGYWWKRKEQLAKKQHHTGSTCR"
     gene            complement(7350..7952)
                     /locus_tag="ACOIAS_001776"
     CDS             complement(7350..7952)
                     /locus_tag="ACOIAS_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2701488.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            complement(8037..8261)
                     /locus_tag="ACOIAS_001777"
     CDS             complement(8037..8261)
                     /locus_tag="ACOIAS_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701489.1"
                     /translation="MSGTYLSIYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFL
                     VVALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="ACOIAS_001778"
     CDS             complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="ACOIAS_001778"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGZ2701490.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNHILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11017..11151)
                     /locus_tag="ACOIAS_001779"
     CDS             complement(11017..11151)
                     /locus_tag="ACOIAS_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HGZ2701491.1"
                     /translation="MWELLKDLLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            complement(11159..12358)
                     /gene="mobT"
                     /locus_tag="ACOIAS_001780"
     CDS             complement(11159..12358)
                     /gene="mobT"
                     /locus_tag="ACOIAS_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HGZ2701492.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPNNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIGYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTQPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            complement(12560..13954)
                     /locus_tag="ACOIAS_001781"
     CDS             complement(12560..13954)
                     /locus_tag="ACOIAS_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HGZ2701493.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFSSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            complement(14071..14556)
                     /locus_tag="ACOIAS_001782"
     CDS             complement(14071..14556)
                     /locus_tag="ACOIAS_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701494.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            complement(14654..15037)
                     /locus_tag="ACOIAS_001783"
     CDS             complement(14654..15037)
                     /locus_tag="ACOIAS_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2701495.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQVGNPQNHAPQGQPKK"
     gene            complement(15052..15378)
                     /locus_tag="ACOIAS_001784"
     CDS             complement(15052..15378)
                     /locus_tag="ACOIAS_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HGZ2701496.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            complement(15614..18658)
                     /locus_tag="ACOIAS_001785"
     CDS             complement(15614..18658)
                     /locus_tag="ACOIAS_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2701497.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTDNHNGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITNGNG
                     NYSIAGATYGVFSDKDCTKQIATLTTDDSGNTEVAEVKATTVYIKELSAPLGYKVDTT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTTAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAFLPL
                     GTLTVEETKSPTGYLLDGAYMQANGSEEQIKALYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETKDTKPQGSATLKDTAFDIISLNDNAVLVEGKIYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSKWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGMELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPMKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            19186..20193
                     /gene="asrA"
                     /locus_tag="ACOIAS_001786"
     CDS             19186..20193
                     /gene="asrA"
                     /locus_tag="ACOIAS_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HGZ2701498.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKDVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            20194..20985
                     /gene="asrB"
                     /locus_tag="ACOIAS_001787"
     CDS             20194..20985
                     /gene="asrB"
                     /locus_tag="ACOIAS_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HGZ2701499.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            20998..21969
                     /gene="asrC"
                     /locus_tag="ACOIAS_001788"
     CDS             20998..21969
                     /gene="asrC"
                     /locus_tag="ACOIAS_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HGZ2701500.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            22026..22802
                     /locus_tag="ACOIAS_001789"
     CDS             22026..22802
                     /locus_tag="ACOIAS_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGZ2701501.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            23073..25091
                     /locus_tag="ACOIAS_001790"
     CDS             23073..25091
                     /locus_tag="ACOIAS_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGZ2701502.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            25306..26163
                     /locus_tag="ACOIAS_001791"
     CDS             25306..26163
                     /locus_tag="ACOIAS_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2701503.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            26260..27129
                     /locus_tag="ACOIAS_001792"
     CDS             26260..27129
                     /locus_tag="ACOIAS_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2701504.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         8262 a         5027 c         5017 g         8964 t
ORIGIN      
        1 attcccacat gggtaacata cgaaccagcg ttataggttg aatggaaaaa gaccaactcc
       61 ccagcttttg cctgcgataa agggatatgc tgggttgcgt catattgcat ttgtgccgtt
      121 ctcggtaaag agataccagc ttttccataa caccattgcg tcagtcccga acagtcaaag
      181 gaagtgttgg gattgctacc gccatacaca tatttccagc cttgatattt caacgcttca
      241 ttcattacct tttgtgccag ttctcccgat acctgcggaa cagttaaata ctgattgact
      301 aattccacat aaaacatatt tccatagcca taccgccagc ccccattttt cgcaacggct
      361 atcgggttgg tgtaggttac ttttttaccg ccagatttct cacgggcaaa gctctctgca
      421 aggttaaaag tatgtttctt tccttttcct gccacatatc ccacatagcc accgccatag
      481 ttataggact gtatcgccac attcaaatca tttattcctt gatttttgca ggaagaaagc
      541 agggacgcaa aatatttaca cccctgcttg attgagcttt ctgtatctaa cgagttgggc
      601 ggtaaaccaa gactttctga actctgcatg acatcttttg ccgtaccgcc actttctact
      661 tgaatgatag ccagtaacac attcacatac tcagaaatac cgtattcttt agcgtacttt
      721 tccaccatag gctgatgttt caagacttct gcggataaat tcatacccgt aatgcccgaa
      781 gaaaagttgc tgttctcgtc gtcgctgtcc gcactcatca gtaccccaaa gaaaaggact
      841 atggaaaaga ggataggaaa cagactgcca atgatagcga tatgtttcaa tttcattttt
      901 tcttctgtcc tttcttcgtt acaaggttac ggttcttttc agtatgttct gttgtacgct
      961 ttgcttgttc aactgtttgt gcctttgtgg tacgttctgc tttgtaattc tggtgtgttt
     1021 catgcgatac cactttttct acatttttac tatgtactgt ctgtgccgat tgtggaattt
     1081 ttccgtcaga tgtacccgaa gataagggac gttctttcac actatttgtt ttgactggct
     1141 cgcctgcttt ggaagttgaa gttgtagcag gacgtttgac ttcctgtgtt ttctcaacat
     1201 ttgtttttgg aatggctgaa gctgtgactg gtcgctcatg gggacgggta gctcccgttg
     1261 tcgctgatcc gtccgtcttt cgtgcctgcc ttgcttcttg tgctttttga agttccatac
     1321 gcttgtcagc gatattcttc ttatgctgtt cctgcttttc caagcgtccc gactgtctgg
     1381 attgttgttc ctgcaccatg ccacgcttga agtccgatac acttgttttt gctttttcct
     1441 ttgcggaatg gacggcataa gcggtctgtg tcggtatatc cttgaccgtt tctttcatag
     1501 cgtttgcctt gtctttgacc ttattcttcg tatctaagac agcacccacc tttgaaccag
     1561 cacgttgtcc catgctggaa gatgtattcc ctcggttgtc ttttgctgaa ttattccttg
     1621 ttgtccttgc attagaaacc gtactgccag ccaccgcacc agcaacaccg ccagcggtaa
     1681 cagcaccagc aagccgtctt tccatacgcc tagccctatg tgccatataa agatacggtc
     1741 tgcggaaaat tcttcgtccc atgctttgac tgtcgttagc gttcaaagag aacatactca
     1801 ttaaatcgcc taacttcatg tagataccag cgaaacacac tatctgcaag aacgccacca
     1861 taaagaacgg gtagtctgtg gaaatgttat aaaacatact ggaaatgcta aatgccaccg
     1921 ttacaatgag cgtaattcct gcccttgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggatagtaa aaagctgata ggtaaaaaca
     2041 tggcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtgat acctaagttg aagaacaaga ggaagaacac catacctaaa cggtttacga
     2161 cttgcggtat cgtcagattg ttgttgtcgt tactttcgat ttctgttttc acgacttcct
     2221 ctctggtttt gccgtcctcg ttctctgggc ttgccgatac aagagcttct acacggtctg
     2281 tcccgatttc ctctgcgttg ctgttcccaa actgtaagag tagccacggc tgttccactt
     2341 gaatagagaa taggctgtcc cttatcaagt ccacgctgtc cttgccctcg ctgtcagagt
     2401 ttgggagcat gatttttgtt cccaagtcca aagaagcggt actgatgtcg cttgaaaatt
     2461 catttatctt tttgatgtag tcgggagcat aggcaataaa tgaagccgac aaaataaaca
     2521 ccacaacaaa gttgataacg gcgtgaagtg ccttgctggt ttctctttta atcagtcccg
     2581 tataggcaac atatagtccc accactaaga taatgagaag caggaaaccg acatagaagc
     2641 cagaggttga aaaaccgctt gaggtaacgc ctgcaagggt ctgtatgctc tgcccgatac
     2701 tgtctgccat atcattgatg aagtccagct tgtaggcttc ctgcactaca tagcccgtgg
     2761 cattacttaa atataaactg atagtccaga caaaattagt gatacagtac aaaccatatt
     2821 gtactgattt tccaatcccg tccagccagt tccacggcaa ccaagcccag ctattatcca
     2881 catagaagtc aagctgatag ttggaaagcg gatatttgga atatagattt tctgcgttta
     2941 tcgtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggagagtgc caccacgaac agagccattt tgagaaagtg gaaaatcttc ttttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtatag gcggtctggt
     3121 atcaaaggcg tgtagcagtt cttcaaagac ggggtgtatc tgtaccacgc cgacacgccc
     3181 gtataagtcc tgcaacaagc attgtccgtt ctccaaatca cgaagtcgct tctgattgtt
     3241 ttcgtcctct ttgtcgatac cgaaaaattc taaagtctgt tttatctcgt tgatgtcagt
     3301 agaacggaaa gcgaatttta agccgatatt gtttttcaga ctttcctttg acacgtcccc
     3361 agaagattgc gtaacgaaat acacacctgc ctgcatagct cgtccagcac gcaccagctt
     3421 gttagataaa atttcgcctt gtgccacatt taagaacgcc cacgcttcat caaggtctac
     3481 gattttgaag atagagcggt cgctgtggat aaaatctaag gcaaaggtac taatcacaat
     3541 cagcatggaa accgatagta attcaatggt cgtgtattcc tcaaaagtcg tgtccttatc
     3601 tggcagtaca aggtcggcta cttgaatgat attgagctga ttatctaaac tgatagcatt
     3661 ttccaccgtt ccgtctgaaa acagaagatg tgcaaaatca tagtctgtaa agctgtcgat
     3721 atggtctgcg atattgcgtg aaattgcggt atcttcacag cgtaactcgt ctatcacatg
     3781 aagcaagccc cttgtttcac tctgggtaac agaacgcacc gccttacgaa gtacggggaa
     3841 tttttcgccg tccctagagg aaataccagt taaaaaggtt agtatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaacgggtca agaagccctg cgttgtcctt
     3961 gtcgcttgtt aggtttacga tattgatttc atgggcgatt tctggaagtg tttctttcca
     4021 gtttccacgc tctgattttg ggtctaagat aaccgcttga ccgccaaaca aaacagaata
     4081 gtacacaagc aggttattac agaatgactt cccaccgcca agcgaaccaa caaaagcaga
     4141 agcaagggca tttgtaactg taccttttac gccttgactg gcaagggacg gttgcagata
     4201 tacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgtgtcgca ccaaaaccaa gcccagctaa gaagtctgat tttacatact gcacataatc
     4321 attgatatat cgcttgctgg ctggtagaaa ttcagaatga agccccagca tatccccagc
     4381 aggacgtact aacttcacat tcaaatcgtc ataaaaatct ttgacttcat cacaacggcg
     4441 tttcagttcg tccaaatcat cagcagacac acggataaca taagagagct tatacatact
     4501 ttcttttgtc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtctactac
     4561 atttgagctg gtttcacttc cagcttgata agcgtgatta tcaaggtctt tcagttcctt
     4621 tttcttgttg cggacggtcg ttaatgcttt tcggttttcc acgatttcta cattcatgct
     4681 cgtatcaacg ggaaaggtaa attgctgttg ctggaaatag aagatttcac tagacggaaa
     4741 atcaagctcg cctacaatcg cattgacggt aaaataggac acataacttt ccttgtcctc
     4801 atgttccaat cgtaaatatc gctggctttc ctcaatcaca caccttgtcg ggcggataag
     4861 gtcgtaatac ttgataagtg tttctttctt atactttttc tttggtagct ggtgctcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc ataagatacc caaagtcatt
     4981 gatttccaga cggcgaacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aagtcgttca tcagcgtgtg
     5101 gttcacttca tggagaaact ctttgaacgt cagccacgcc gattttttaa ggttcttcag
     5161 attgagctgt tcttccgtaa ccatgagttt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatcttct ggtaagcgac
     5281 ttcctttaat ttccccgtaa ccaatctttt tgactgctcc tgcatacttc ttatagagct
     5341 ttccgtggca atctgcaaag catgaatttt accctcacgg gactgtgcga ttagctgtcg
     5401 gaaactgtca tgcacgataa atttctgttc tgcggataag aaagaatagt tatacggtat
     5461 cagctcatag taggcgaaca cctcattgtc cttgttccag acaaggttgt tgtcaatata
     5521 tttaatcggg aacataactc acactcctta ctgccgtgat tgtttcgttg aatatttctt
     5581 tttgcagttt tacggctttt cctgcataag tgattttcgg tcgcaacgca taggttatct
     5641 gtgatttcaa aaagctgtat ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgaggaaagc aggaataccg aagtatttta aaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ctgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggcaag ttaaagtcat tgattgcata caagactttt tctacattcc
     5881 agatacccgt gtagcttcta atctttttca agttctttca actcctctca taagtttcta
     5941 aatggaaaag gacagccctg tttcagactg tcccttaaag agaaatacgc cgtcagtagc
     6001 aacaatgctt gtcgctgatc ttccagcgtt aatatggtat ctcgcacatt cctcggcttg
     6061 ttgcaatgaa gcaaccgccc atatccaaat ctcgcccgta tgcttcatag tcaatatagt
     6121 tctgcaagct agggggaata tcgccaagtg cctgcaattc atctatgtag taataggcaa
     6181 catctgtcat ggtttcacag tcgggataat agtaaatatc gtccttgtgt tccacgactt
     6241 cctccagcgt cccataatga ccaataaatt cgtccaaaca atccacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcatcaat ggaaacatac tccccaatct
     6361 caatcgggaa gttatcggta tcatggattg cgtattcttc atactgttca ttcaagccga
     6421 ttttttcttt cacatcttcc tcgtcaatag ggaaagtaaa ccaagcaccg actaaatagc
     6481 cctcattata cttgccaaga ttagcgatat aaactgccat atcatcaatc atacttactc
     6541 tggcaattca aagataccgt gttccgttac taaaaatttc ccgtgttcct gcaagtgtga
     6601 agcataggtt tcaaagtcaa aatagtattc ttgacagtcc tcggataaat gtttgaatgt
     6661 agggtcattc atcagctttt gccttgcaac atcaatcatg cttttacagt cgggataaag
     6721 cgtaattaca ttcctgcaaa tataaagtgc ttctaaattt tcatacaccg tatgaagcat
     6781 tgtatattct tcctgcatat cgcttgaaag ctggcgatac atataatcta attcattgag
     6841 ctggtacacg cttgtatgct catggacttc atcagcataa ggcaacaatt tttcaataat
     6901 gcggtaatct tgactttctg tactgacacc taacttttct tcaaactcgg caacctctat
     6961 cggcaggtcg aaccagtatg atgttgtttc ttcgccagtt gttcttttcg tttccaccag
     7021 tacccttgtt tcctgcattg ttcctcacgt cctttctgtt gtttcatcac atcttttaaa
     7081 gtctggcatg acataccaaa cacatatttt gaaaaatctt ctaactgttt ttcttcatag
     7141 tcggcaaggt tcagttgctt catttcatgc cacaccttac gcttgtaaga gggcaggtct
     7201 gaaatgtttt cacaaccaac ttttgcctgc atatctctaa aaatatcgtt cattacatca
     7261 ctccaatctg aaatttaggt aagaaaaaag cagtcaatcc taagactaac tgctttgtgt
     7321 gtatggatat gaagttgtca aactgggatt tatctggcag acttgtttat ccactcatca
     7381 agaaatttca tttgttcttc cgtgtgaaac caatgctctc cgtttctcat aacagtaagt
     7441 gttgcattag tttgttttgt aaattcagat atagttgcat aagaagtcaa gttatctttc
     7501 tcaccaaata aaatatttgt tggaattttc catgtagttg gattttcttt tacataacag
     7561 agatattccc atgagagtgt ttctccaaaa gttgttttaa tttctttttt atctcgaagt
     7621 tcatcttctg taacatttgc ccaatacatc atatctgcaa tcagtttttc catattcaca
     7681 ataggtgaaa taaaatatgc tttttctatt tgcttatctg ataaggcgtt catagcgaaa
     7741 taggcaccta tgctatttgc aactatttga acagatttgt aatgtttgca aatcaaatca
     7801 aaaagtgatg gaaattcttc ttttgcttcc catggatatt gtgcagtata gttgagtcca
     7861 accacatcac aatcactaaa aagaggtttg taatgtgaag actcctctgc atttccacct
     7921 ttaccatgta tataaataac tactttatcc ataatagttg tcctcctcaa cttcaaattt
     7981 gtcgctttct tcatatccac attatatcat tctcatatca gcaccacaat aaaattttac
     8041 gcaccgataa tcttattgaa cagctctaac agtacgtctt tcacaccgcc agcattgaat
     8101 accaatccaa cggcaacaag ggcaactacc aagaagccga taagtttaga aaactcacgt
     8161 ttgaacccta agtagatacc aataaccaca atcgccataa gcactaagct ctgggcgttg
     8221 cttaaaaacc atgtgtatat ggataggtaa gttcctgata ttataaatat ctcaaacttt
     8281 tccccccatt cacacggagc gtgcgacttt caacgcactc cgcgttccat cgtgtttata
     8341 ttgaaaagta tttatgtagt agacctatat caagtttaat tcttgattgc cagctaatac
     8401 tctatattta tttagtctta tgagctggac tttatttagc ttcaatagag ctatatattt
     8461 ttgtattgtt tcttctgttt tagcgtgtat tagacggtgt accgcctctt ttaccaaaat
     8521 atgattttta taatcgtcat ttccaccagc tccagttggt acaatatgat gacaatgtat
     8581 ttcattagct tgttgaaatt cttccagagt tatagcacat tttccatgtt gagcagaaaa
     8641 tagcgaaatt cgattgtctg cgtattctat tgtgcgattt tccaaagata atttcattaa
     8701 ctggtacatc agtaatttat caacacttag attggtgtgg ataaattttc ttccctctag
     8761 agtgtagcaa ttcgctaaat acacctttgc cattggtttt ctgtgttgga tatatccaac
     8821 agggaaaata ggttcgccct cgccagcaac ataccggagc attttagatt taccatatct
     8881 tgttgcctcc tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc ttttgtttgt
     8941 acctaatctg ttagtgaaga tagccattac agctctattc agtttcatac aatccagatt
     9001 tacattcgtt gcaatctgat aataattttg catacctaat accattgaat tataaagtct
     9061 tgtttcttct aattccgttt tcccttttga gggagaagca attttctttg cctgtgatac
     9121 cagcttgtct ttttggtttt tgaattgttt atcacttata tgtgatttaa caactaactt
     9181 ctttcttctc ggtatcattt taatcttgaa accaagaaag tcggagtatc tttttctaac
     9241 atttacaatt cttgtttttt cttgtgatac ttccaatttt aatcgttgtt caatccattg
     9301 agtgattgct attttggctt tttcagcact ttccttgtag cggcagaaaa ctctaaaatc
     9361 atctgcataa cgaaccatat acatttcctt taactttgtt ttcttcattt ctttgtaagc
     9421 gttgctttta catgggctac cgcttttatt gatatgaacc acatagtttt ttgtaacagg
     9481 attttcctgc cattggcttt ctacccaatg gtctaactca ttaagaacaa tgtttgctag
     9541 tagtggggat attattcccc cttgcggagt tcctttctct ggatatgtta ttgtaccgtt
     9601 agtcattttg attggagctt ttaatatcct ttttaaaatg aatatcaagt gcttgtcccg
     9661 tattcccata gcccatatct gttttatcag cttggaatgg ttcacgttgt caaagaaacc
     9721 cttaatatca aattcaatca cataatgtag ctggctgatt tgtaggcggt tataggttgc
     9781 ttttattgca ttttcaactg acctattagg tctgaaacca tagctgtttt cactaaattt
     9841 agcttcgcag attggttcta atacttgttt tatacattgt tgtataagcc tgtcccacat
     9901 acaaggaata cctagaggtc tggttttacc atttggtttt ggtatttctt ttcgcctcac
     9961 tggcttaggt cgatacccat gtggactgcc tgcaacaatg tatcttactt ttttgacaac
    10021 tgtttctgct ggcaatttgc caatatcttt aattattgta ccgtctgttc ctgctgtttt
    10081 actgcctgca tttgctttga tatttcgata tgccagtaat atattttctc tcgataggat
    10141 aatatccata agatgagtga aaatatctcc attcttactt ttggcatata aatcatcaaa
    10201 ggtgttttgc atttcgtaat attcagcgtg tcttaaatct tctacacaca atgtttcatt
    10261 tttattttta ggcataaggc ttcactcctt tgcttgaaat tgagtgagct tttttatctt
    10321 actcgtaatc cttattgaaa caacataata cttttctaat atatacacga cttgagggca
    10381 ttcctccatt ctcattacaa gaatttctta ggttcgccct ctgcttttca gcattagttc
    10441 aactgcttat tgttcttcgt cagtttattc tcttgatgag aacccaatac ctttcctcgt
    10501 tccgataatc ttatctatac atatatatcc ttaggtcact agctataagc ctgctggctt
    10561 gataccgcct gtaacgttat atggcatttc ataacctata ttcttacttt gccacaccag
    10621 ctacgatttc tcgtaccttt gcatttctac aaagttcaaa tttagacccg tacattcgct
    10681 gtttcgtcag ttccttatag aacattctaa ccatagatat tttatagacc cccgacctgt
    10741 tggatacgct acccgcctct ggatagtttt cgatagaaga tatatgttca tcaactatta
    10801 cggtatcttt cagccgtctt taccgagctt caaacattct gagtttcctc atacaatgct
    10861 tgtcggaggc tcagggcagg cgtttcagag cgttacttca tcattccacc tgtcagatta
    10921 tcagttctcc taataaccaa actttttcgt tcttttatta gtgccttagc ttttcactaa
    10981 ggaacgagtc gcacataaat tttgtccgaa attcatttaa ttgtcctctc tttctttgat
    11041 ttcttccata catctgtcag cttctttgcc gacagttaaa atgcacatca agactacacc
    11101 gatacccatt ccaaatgata ccagcaataa gtctttcaat aattcccaca ttttttcatc
    11161 actcctttac gttggcaacc atttcttcaa ccgtggctgt ctgttgcttg attatctggt
    11221 agtgtcgttc ggtcagtttt gcctgctttt caatcgtttc cagatactcc gtctgattgt
    11281 ccttgtctat ctgcttcaac atcttcaagg tcggtgcaac ctgcctttgt agccagttga
    11341 gtgtacgctc cagcgtgtaa ggttctggct gtgttgtcag ctttacgggc ggtcgcccct
    11401 cgccaatgaa ccaagcccag cgtacattgg ttttccagtc gctttttcgc ttgtctggtt
    11461 ctttatcagc gaagcggata tattgattga taatagaaaa tgctgtcagt tctgcgtcat
    11521 aatgtgtcag cagttccaca acagcataat aggcacgttc atttttcaaa cgtatctcaa
    11581 atctattttt tatggggact tcctcaatcg gtatgccaag tttggcgtac tgttcgtaat
    11641 tcttttcata acagcagaag tacacttcac ttgaaaatga accaatatac agcgtgtgtc
    11701 ccataccgta tttatccgtt tcattatgct ttaccagctc tccagaacca tagcttttga
    11761 aagaacggaa tttagaaata cattcctcat tctcgcattt tcgtatcagc tctggaatat
    11821 caagtaagcc cgttcggtca ttgattgcca aatcaagacg cttcattaca ccgccctcaa
    11881 ccaatgcgtc cgtgaagaag tcataccaac ttcgctcttg tgccagcaga tagctttcaa
    11941 actggcgaca gcctttgcct ttcagttcca gcaggacacc tttttcttcg tcctgcgata
    12001 catacacaaa cacatctccg atatggtaat gctcggtgta tttgtaatgt ccgtaatctt
    12061 cgtgaagcat atagccgatg ttgagcttca atatatcctt gatgatatgc tttatatcca
    12121 ttgtgggaaa acggatacgc acataatcaa acagcaggaa catgggattg ttgggattga
    12181 atttttccac cgcttctaaa agcgtatgtt tcatgtcctc ggtcagcgtt acctttcctg
    12241 cttcgatacg gctcaaatgt tcacggctga tatttgccat gattgcaagc cttgtctgcg
    12301 atacgccgta tgcacagcgt ttttctttca aggcggttaa aaaatcagtt tcattcagtt
    12361 gtcatacccc caatcgtgat aattttgtga tattgtgata atttcaaggc tttatcacaa
    12421 aaggcgaaat gtacgaaaaa cccttatttc atgcggtctt gcaaaggttt ttgtggaagt
    12481 ttccgccgta attgtgcccc tctgttagat aatgaggggc ttgtatctgc tggcgtggct
    12541 aacgccacac cagcaacggc tagtccgtgc cgtcgccttt cgcttcgcac gtcgccgtcc
    12601 cgtcctgcct tgcgtatgca agtgagccta tggtctgcaa aaaatcatgt ccttttggaa
    12661 ctaagggtgt gtaaaattca cttatgacgc ttgttcccac atcacaatag ccacgtccct
    12721 tgatacgctt ctggaaaaac tgttttttca catcagaact gaacagcata ccgtaaccta
    12781 attcgctgat acgccccaag ccaacacgga aattaaaatt atctctgata ccgtccgaga
    12841 aatattttgc gtctggacgc tggcaggcaa caataaggaa atatcctgct tgtcgtccta
    12901 acatgacgat tttctttagc tggctaagta ggctcacact ttcttttgtc cccagcattt
    12961 caaaaaacgc cacatattca tcaaagataa gaaagcaggg cggtaaacca agataggcat
    13021 agttttcgcc cgttttatag tctgggtgtc gcttcatttc ttcgcttctt tgtaccatgc
    13081 cctcataaaa ggcattgaca caatcaatca tttcttcttt ggtgtgatac acatttccca
    13141 ttaccgtacc taaatctgca aggtcagcgt tcttcgggtc taatatgtaa agaacagcgt
    13201 tcgtatgtaa cagagcttca ataagcgtca gcagaaaata cgttttaccg ccaccagtcc
    13261 caccagcaat cagagcgtga gggagtgcgt catattccca tacaagattt ttcatcagcc
    13321 taagacagcc gttttctgct ttcacttcat caatggtaat gcggttcgct atcatatcat
    13381 aaagcatggt gtattcgata taaccgtcat gtaacgtctt gtcggtcagc tcacaataca
    13441 agccactttc caatttatcc tctaaccgta agagctggtc ttgatatttt cctaatgtaa
    13501 tttcacagcg gatatgaagc agtccttttt ccatttgata ataaatcttt ggaaaccaga
    13561 tgattttttc ccttgatctg ctttgcaggt cagtaaaaaa accgctgtct tgtacggtgt
    13621 ctgcttcata ccacttgttt tccagtatca ttcgtgccag cttttgacgg tgcaggagct
    13681 tcttgaaact gtcataacag aagcggtaat acagaaaagc gaccaatgca caaacaccag
    13741 tcgctatcag tagggttatg aagttgtaag tggaaagcgt caaaccgttg tctaataagc
    13801 tgaaatgctc ccaatcggta cgcatgagct gtttcatatt cagtagcagg ataaccgcaa
    13861 tgaatacaaa cagaagcgtc cctatggaaa agtggtagac aaggttcttg tcgctggctc
    13921 tgatacggtg tcctttgttt aaaatcattt tcataaatca atcactcctt tctgggtacg
    13981 aaaaaagcag tcaatcctaa gactaactgc tttgtgtgta tggatatgaa gttgtcaaac
    14041 tgggatttat tgttcttaac ttttctgtcg ttataaagct gacttacgca cattaacctc
    14101 tgcacagacc agcccagcta tatgctccgc tgtttcattt gtcagtacaa agagcattcc
    14161 gtcattacgg cattttttat gtgtgtcgcc attcaaggca taaacaaaac cttttacaca
    14221 ggtatcacta caggcattag ggtcaatcag tctcttgcag tcacgagaag tagtcatttt
    14281 cttttgcata tcagcaggaa gcgaagcgat tatatcctca tactttccag cgttgtctcc
    14341 gtaaatccgt gcaaaaacac ctgtcttgcg aaacacccaa ttcattactg ttttcttctc
    14401 tagttggtat gaagcggtgt aaccgctttt tgcttccttt attacaaggt cacagccctg
    14461 ctcaataagt ttggtattaa gtttctcgac aaacgcttgg tgttctggcg cgattgccga
    14521 tagaaattct ttgaatgaac ctttttcttt tgccattatc tttttcctcc acaaatttca
    14581 atttatccgt ttcttcatac acatattata acagttgctt atatccgcta caatgaaaat
    14641 ctagtttgcc agcttatttc tttggttgcc cctgcggtgc atggttctgt ggattgccta
    14701 cttgaccgcc tttattcttc aagataatat cgtcagcctt gatataccag tccacattga
    14761 cacctcgata gctggcgttt gctaccgtat cggctacggg attgacaagc tctacctctg
    14821 cattatacgg atagtccttg acgggaatat cagcaggaat tgatacttgt atcatacgtt
    14881 cctgcacact acatttcaaa tcataggttc ttcgtgtgat ttcttcactt gttgaaccgt
    14941 cgtcattttc tgctcgcacc tcatgtctga gggcagagaa cttcaataca ccgaaagttt
    15001 tttccttgtc taatacaata ccgtttgcta atctcataat catttacccc cttacttgct
    15061 ttcctcggct ggcaacatat catcagccgt taagatatag tttgtgaacc ctcgtccttt
    15121 gaccgcttta ccgtctgcgg taatctttgg attgataagt tttactcgct gttctggttt
    15181 gaaatgcttt tcgcctgctt tcgctggaat ggttacgata atatcatcag ccctttgtac
    15241 gtcagagtac aagttgtagc ttcttgatac gggcgtaaag cgtccgttaa tgcgttgctg
    15301 ttcaatcttg ttttcgccag caaattccaa gtttccaaaa gtctgtgcca tgttcggcac
    15361 tacatatttt aattccatag tgttttacct cgtttctttc ttctttttta agtttgttga
    15421 ttgtgctttc ttattctggg aattagggaa ataccagtaa tcccccagat agtaaagggt
    15481 aaatcaatgc ttcgcaccct tgactatccg tgtgcttgca ggttgttggc agacaagcca
    15541 gaataagtaa acgtctgccg tttgacagct tcggcagaga gcgtgatttt tcttttctca
    15601 taccgttacc gccttatgat ttcttcattt tacggcgttt gtaaagatat gttccagcca
    15661 gtccaccagc agatgtaagc aacattacca gaaatgccat gacatttgtg ttgtcgcccg
    15721 ttttaggact gtctgttggt ctgctcggtt tttctggtgt tgttggcgtt tcgggtttct
    15781 ctgggacttc tggtttttcc ttaaaagtaa tcgtctgtcc ctcgtcctca atgtccttat
    15841 gctcagtaac ttttttcggc tcgtttggat tgcttaaatc atacaattct tcaaaagtta
    15901 caagctgttt gccgtcaaga gaagtagcgt caaaggtaaa ggtaacttcc actttcatag
    15961 tttcgctgtc agcgataaac gtataatcac tttccacacg ttttccattt ataagaagct
    16021 ctgcattttc ttctttcaac atctgccagc ccacaagttt atactttgta ccaacttcca
    16081 agccctctaa ggttacagta tcaatgattg ttacgtcttt tcctgcttca agctctttgt
    16141 taccgtcctt atcggtagca gttgtatgta ttttgatgat acgttctgta ataagtaccg
    16201 tctgcccgtc gtcctcaata tctttatgct ccgcaacttt catgggttcg tctggattgc
    16261 ttaagtcgta taattcctcg aatgttacca gatttttacc gccaagctcg gacgcattga
    16321 atgtataaga aatttccact ttcatttctt catcatcagc aacaaaggta taatcgcttt
    16381 ctacacgttt cccgtcaatg ataatctcgg cgttttcttc ctttaacatc tgccagcctt
    16441 ttaactgata cttagtgcct tttgtaagtc cgtctaattt gacagtatca acgattgtta
    16501 cctcttttcc tgcaaggatt gtcttttcgc cgtctttact ggttgctgtg gtatggatag
    16561 tgatttcctt ttcgtattca tcagtcaaag tccctaaatc aatcacaaga ttatttcttg
    16621 ataccacgat ttcaaatggt gggataagtt ccatgccttt gttgctgtca gagcgtaatt
    16681 cttcaataat gtaagtatcg taaggcaacg cacctttgct gtcgtctggt tcagaagttc
    16741 caaaccatac accgtcctcg cttgttttgc cagcgttggt attgtgcttg tgcgaagccc
    16801 attttgaaga agtggagaat tgcccattat cgtcagttac cacaacatga ctttcgcccg
    16861 ttgtcttgct tgtgatccta aagggaacat cagcaagacg cttgtgtgtg cctgcaccga
    16921 ttttcacgcc ctcaatatcc ccacgcttaa tctgattgta gatagagtgg gcttcgtctg
    16981 ttaagtccac gatttttccg ttttctgtga ttgtaaaatc aatcggcttc gcaccgtctg
    17041 tcaagtaacc gtttggagct tgtttctcaa cgatacggaa attcccgtaa ggcaaaaggt
    17101 cagcagaagt agaagcgata ccctcaatat ccgtatggat tgtttttaca acttcgtttt
    17161 tcttgtatat cttgccctca accaataccg cattatcatt taaggaaatg atgtcaaagg
    17221 cagtatcttt caaagtggca cttccttgtg gtttggtatc tttggtttct aaatctcgtt
    17281 tctgaatttt gacaccgcca cgaatgactt tatctgatac ggaaaactgg ttacttccag
    17341 ttaatacggc aaggtcgccg tcctcggtaa tctgcgtaag gtataaggct tttatctgtt
    17401 cttcactacc attcgcctgc atatatgcac cgtctagtaa gtagcccgtt ggcgatttcg
    17461 tttcttcgac cgttagtgta ccaagaggta aaaacgcttt tccgtcctgc atatagaagc
    17521 tatcgccaga taccttgtat gcgtctgcta attttgtgat gtaatgggtt gtcccgttac
    17581 tgtctgtttc agcggttgtc tttgtaaccc atgtacgagt agcttcggca ggaagattgt
    17641 ctttattgta gaagcctgca taatatttcc atgtaaactc cgccccctct aaagaagcgt
    17701 tcccttgtgg gttggctttc tgtgtttcca tgtcaatttt aaaaagctca atcaaagtat
    17761 cggtaacttt tggcgtatca gatactttca aagtcgctgt ctttccagct tcgactttta
    17821 aggaatacac cgtagtatca actttatacc ccaatggtgc agataattct ttgatatata
    17881 cggtagtagc tttgacttct gcaacctctg tatttccgct gtcgtcagtc gtaagggtag
    17941 caatctgttt ggtgcaatcc ttatcggaaa agacaccgta agtcgcacca gcgatagagt
    18001 aatttccgtt accgttagta atgctggtat tattggaagt cttttgaagt ttggcatttc
    18061 cgacatttaa cttcgcccag aattgcccta attcctgccc ctcgccagag tagatataac
    18121 cgccgcattc atagcgtcct ttgttttctt tcacaaaggc ttttgcacca gcaaacactt
    18181 catcttgtgt tgcctttggt atttcatcat aagaagctcg cacgttatca cattgccagc
    18241 ctaagtgaac actcaatctt tgccacacaa caagctgtct taaaaggtag gcgtgatttt
    18301 tgcttatccc attgtggttg tcggtgtatt gcttcacata ttcgatagat aaggcaacat
    18361 cacttatctg gtcggcactc atacgggagc ttgcgtcagc tctggtcttg tagccgttct
    18421 taaaatctgt gttaatatcg atacaataag cgtcctcgcc ctcaacggtc aaatgcccct
    18481 cgttaaaggt agaaccaatc gaaccgtcat tcattacttt ttcaacgata ccgacacgtt
    18541 cttcactttc cgtccagtat tgcttgcttt ctgcatggac aagtgtagtc ggtaaagcag
    18601 taacgacagt cgcaaaggca agtacgcccg tacacaatcg tttcaatatc tttttcataa
    18661 ttctaaatgc tcctttcatt ttgggtaata aaatagccgt ccataatgaa cggctggaaa
    18721 tagaaaagga acgtcaattt aggcgttcca tagtctatga aatattcaat ttttttcttg
    18781 cttcaatact gatgtgccgt accatatctt tgcatatcta ggaaaacttg cgtatcaagg
    18841 ctcattcatt ggtagtaaat tagtagtaaa ttgatgtgtt tgtttccttg aaaatgctga
    18901 tagatactat gttttagcgg ataatcaact ttttagtatg ttttttgcat aaaaattgag
    18961 tagatagtag ttttattgta cttaatgatt gtatttaagt gattagattt gaaattgaat
    19021 aatgtagata ataaaaaaat attaacagaa cttaataaac tccattaata tttttttatt
    19081 atttttgaaa ctgtgataat tatcacggaa gcatagaaaa taaagtgtta agatttaaac
    19141 aagatagtaa gtaagtaaaa ctaaaatata agaaaggtga taaaaatgaa actaaaatta
    19201 gattcatcta agttcaatga aggtctgaat tatctaaaaa aagactacaa gatatttgca
    19261 ccagttttaa taccatttaa aggtactttt tcagatactg atgttataag atacaaggag
    19321 gtaaacgctt ttgaagaaat ggaatttgct aagaaagcaa acttttcacc aaaagatgtt
    19381 gtacttccca taaatcaagt attgttttat tttactgaaa aagagtttaa ggaaagcgat
    19441 ttagacaaca agaaaatttt aattttctta agagcttgtg acataaatgg tataaaaaga
    19501 cttgatgaaa tatatctaaa taatggtgaa gaaaaagatt atttttataa aaatatcaga
    19561 gataaagtaa agtttgcact tgtagggtgt aaggaaagtt ttagaaactg tttttgtgtg
    19621 agcatggatt ctaacaaaac tgataattat tcaattggat taaatataga aggagataca
    19681 ctgtacttag atataaagga tagtgaattt gaagtattta atggagaagc aactgaattt
    19741 aatgttgatt atgtaactga taatttaata tctgtagatg tgccagagaa tattgattca
    19801 aatgaaatga taggaaattc aatatgggat gaatatgata caagatgtat aggatgtggt
    19861 agatgtaatt ttgtatgtcc aacatgttca tgttttacaa tgcaggatat tttctataag
    19921 gaaaacgaaa atgtaggtga gagaagaagg gtttgggctt cttgtcaagt agatggatat
    19981 acagatatag caggtggaaa ttcttttaga aaaaaacaag gcgaaagaat gagatttaaa
    20041 actatgcata aaatacatga ttttaaaaaa agattcggat atcatatgtg tgttggctgt
    20101 ggaagatgtg atgatgcctg tccgcaatac atatcatttt caaagtgtat agaaaaaata
    20161 aatgatttag taattagtaa ggaggaagta taaatgaacc catatatacc agtaagtgca
    20221 aaaatactag atatagttaa acatactgat aaagaatgga cttttagagt aaactgcaat
    20281 acaaaaggtg tattgcctgg aaagttttat gagatatcta tacctaaata cggtgaaagt
    20341 ccaatatcag tatcaggata tggagaggat tatattgact ttacaataag aaatgtagga
    20401 aaagtaacta gcgagttgtt taattataaa gaaggagatt ctttttttat aagaggtcct
    20461 tatggaaatg gatttgatgt atccctttat gaaggaagag aaattgtagt agtggcagga
    20521 ggaagtgcat tagcccctgt aagaggaata gttgaatatt tttataataa taaagaaaaa
    20581 tgcgaaaagt ttaaattaat agtaggattt aaatcaccaa aagatatttt atttgcagat
    20641 gatttaaaga gatggagtaa aaaacttgat attttagtta ctgtagatgg agcagaggaa
    20701 ggatatagtg gtaatgtagg attagtaaca aaatacatac cagaattgga tatgaaagat
    20761 ataaacaata cttctatagt tgtagttgga cctccaatga tgatgaaatt tacagtagaa
    20821 gaatttttaa aaagagattt agatgaaaag aacatttggg tttcttatga aagaaaaatg
    20881 tgttgtggaa taggtaaatg tggacattgt aaaatggatg atacttacat atgtttagat
    20941 ggaccagtat ttgattattc atatgctaaa aatcttattg attagggggt gaagtttgtg
    21001 ataagagatt taaataccaa aaaagtaatg aaaaatgctt atagaattac aaaaacaaaa
    21061 tatgagactt ctttaagagt tagagtacct ggaggtctta ttgaccctga gagtttagct
    21121 gtagtttcta aaattgcaag tgagtacggc aatggacagg tgcacataac tacaagacag
    21181 gggtttgaaa tattaggaat aaatatggaa gatatggaag aaataaataa aattatacaa
    21241 cctgttattg aaaaaatgaa tataaatcaa agtgagaaaa acgcgggtta tccagcagca
    21301 ggtacaagaa atatagcagc ttgtatagga aataaagtct gtccaaaggc tcaatataat
    21361 acaactgaat ttgctaaaag aatagaaaaa gctatatttc caaatgattt acattttaaa
    21421 gtggctttaa ctggttgtcc aaatgattgt ataaaagcta gaatgaatga ctttggaatt
    21481 attggtatgg ctttaccaat atatgaaaag gatagatgtg taaattgtgg agcatgtgta
    21541 aaaaaatgtt ctaagatatc tgttggagca ttaaaaacag aaaataataa ggtggtaaga
    21601 gataaagata agtgtatagg ctgtggagag tgtgtactaa attgcccaac aaatgcatgg
    21661 actagagatg agaaaaaata ctatagatta gctataatgg gtagaacagg aaagaaaaac
    21721 cctagactag cagaagattt tcttttatgg gtagatgagg acagcataat aaagataata
    21781 cttaatactt atagatatgt agaaaagtat atagataaag atgcaccagg aggaaaagaa
    21841 catataggat atataattga tagaacagga tttatggaat ttaaaaaatg ggcacttgaa
    21901 ggtgtagagc ttccagaaat aactaaaatg tacgaaaaca tatactggag tggaattaaa
    21961 tatctataaa ttgagtaaaa catatatttg aataaattaa aatttaatag aaggaagagg
    22021 gagttatgca taaagaaact ttggataaat taactaatgc agctataaat aagataaatt
    22081 tattaaacac aagtaaagta aaatatttgg tatcttctgc gtttgcagga ctttatgtag
    22141 gtataggtat acttttgata tttactattg gaggactttt gactgatgca ggttcaccta
    22201 tgactaagat agtaatgggg ttatcatttg caatagcact aagtttggtt ataatgacgg
    22261 gaacagagtt atttactgga aacaatatgg ttatgtctgc tggtatgtta aataaaggtg
    22321 taagtataaa agatacttct aaaatatggg catacagttg ggtaggtaat ttaatcggag
    22381 ctttggtttt aggcattata tttgtaggaa ctggtctagt agacaaagga cctgtagctg
    22441 aattttttgc aaatacagca gctagtaaag catccatgcc atttacagct cttttcttta
    22501 gagggatttt atgtaatatt ctagtatgtg tatcagtgtt gtgttcgttt agaactaata
    22561 gtgatactgc aaaaataatt atgatattct tatgtttatt tgcttttata acatcaggtt
    22621 ttgagcatag tgtagcaaat atgacaattt atagtgtttc attattttca ccaacaataa
    22681 gtacagttac tattggagga gctatttata atttagtagc tgtaacactt ggaaatattg
    22741 ttggaggagc tttatttatg ggtcttggaa cttatatatt aggtaaagaa aagcttaatt
    22801 aaatttattg tataaattat aactctcttg ataaatacat ttttagtatt gttgagagag
    22861 ttatttttat atttttgaaa aaatatttga aacaaaaaaa atatgggagc atacgaaaat
    22921 atatcggtta tatatataaa aaatacgttt atatatgaat tatatagttc taaatataaa
    22981 aaaagttaaa gtcctaccgt aaaaaagcgc attttgaata agataacaaa tatgataaaa
    23041 ttggtcttgt tgaaattttt aggggggata tgatgacaaa gaaactagta atatcaaaaa
    23101 caaagttgtt tatttttata atagcattta tttctatatt tatatattta tttggaagta
    23161 aaaatacact tataggtgta ggtatagtaa cagctatgct aacactcttg gaaagagatt
    23221 taactatttc tccaattaaa aatttactga agtatttagc aataaatata atcttaggaa
    23281 tactatcatt ttttgcagta caaaatatgt atttaggagt tttattaaat tttatagcat
    23341 tatttataat agggtatgta tttagttatg acttaaaaag agcagtatat gtaccctttg
    23401 gtctaatgta tatatttatg gtaagtatac ctgtaggaaa aagtgaattt cctatgagat
    23461 tgtcggcttt agctgttgga gcagttataa taatgattgc tcaatttgtt atgaatagaa
    23521 ataggatgaa aaatgtcgga gataaagaac tgatatctat atgtgatgaa ttattagaaa
    23581 aaattagcct tttaaaaaat actattaatg atgatagttc tataattaaa tctagtatga
    23641 gaaaaataga tagctgtaat tatagaataa attctatatc taaaaatttg aaaatggtta
    23701 tttttgataa tagaaaagat gacttttata tcagtattag aggtatagac ataatgaata
    23761 ttttattttc attagaaaga ataagtttaa tcctggaaag atacaaaaag gatagtaaag
    23821 aatttgaaga tgaagatata aaaaatattt tagaagaaag tagcataaat agtaaatcag
    23881 atgttttagt tgtagcaacg aaagaaataa actatattaa gatgtgttta gaaaataaag
    23941 ataccataac tgataaagag atattaggat ttagagatta tgttattgct caggatacta
    24001 aaaatataaa tttaaaagaa atttatagtg ttttagaaaa tttatatgag tttttagtag
    24061 attataaaaa agtaaaatca agagacgaaa agaaagctga aagaaaatca aagatacctc
    24121 atgaatttaa acgactatca atatataaga aaaactttaa tttaaattct ataagatttt
    24181 cttatgcagt aaagattgct ttagctacag ctgtagcggg ttttattatg gattatttcc
    24241 atttaagaga tggaagatgg ataatgttaa ctgtcttttc attaacacaa ccttacgcag
    24301 aaaattgtat acaaaggtct agaaaaagaa tagaaggtac atttatagga gctgtaatat
    24361 ttattgtatt attttcaata ataaaagata gtacattaag gtcgttaata gttcttttag
    24421 cagggtatat aaactcttat gtagttgatt atagaaaatt aatggtatgt gtaactgttt
    24481 cagctcttgg ttcagcagta gttatgggag acccaaatgt tttaacaata agcaggattt
    24541 catatgtagc attaggagca attattgcat taatagtaaa taagtttata ttaccatatg
    24601 atgctaaaac aggctatcaa catgttatag aaatgtataa aggaatagtt aaaaatataa
    24661 ttgatgaagt caataagtct atagaaaatg tagctgatgt ttactatata aaaaatttat
    24721 tattgatacc atcacttatt gaagatagac ttatgttgat aaatgctata tataaagata
    24781 aacatcaaga ggatttttta gaaaatcaga aattactaat aagtaatatg tataatctat
    24841 atataaatgt taataaaaat aaaataaaag atgaagatgt tgaaaaaata ttgagagata
    24901 ccaattatat ttcaaattac aatgtagata agtatgatga aggaagaagt gtaattttag
    24961 agagtattgt aaatactaaa agtctaggtg acaagataat atgtctaaac ttactgcaaa
    25021 ctctaaatgg tgtcaaagaa atgtatagaa taagtaatat aactaaagtt tcaataaaag
    25081 aagcagctta gatattaaat tatagaatag ccatatattt aaaataaagt atatggctat
    25141 ttgttttata aaatactaaa agaaaataat atataaaaaa caaaaaacat atatttatga
    25201 attttatatg aaaatagtta aaatattgat ttttttcatt ggttgaaaaa acaatttact
    25261 taggataaac tataatagta ttcaaatatt atgggggtta gatttatgag taaagatgta
    25321 aaggtaaagc gtaataagaa atatgataca tttagtataa ctatggaact tgagagacct
    25381 ttactttatg atgaggttga gaacaaagat gataaaaata ataatagaaa atttaacata
    25441 aagaataaga tatttaaggg aataggtata ctatttgtat tgattttgct tggctttttc
    25501 atatggataa ataaaacata tgaacctcaa aagctggcaa aagaagctct agtcagcaat
    25561 agtaaagttg aagtaactgt aaatgaaaac atttcattta caccaaaagg aagaaaagtt
    25621 tctaaaggtt taattttgta tccaggagct aaagtagaaa tagaatctta tgctccactt
    25681 gcaagaaaaa tagcagaaag tggatatgag gttgtaatcg taaagatgcc acttaatcta
    25741 gctatacttg gaactaataa ggcacagaaa gttatggatt cttacaacaa catagaacat
    25801 tgggtaatag gaggtcattc attaggagga gttgcagcat caaattttgc acgtgataat
    25861 aaattaattg aaggcgttgt atttttagct tcttatccaa tgggtgatga gttgaaagaa
    25921 ttaggaaaaa aagttatatc tatatggggg agcaaagatg gagtagtcaa ttttaaaagt
    25981 cttgtagaat caaaacaaaa acttcctgat gatacaactt atgttgagat tgaaggagga
    26041 aatcatgcac aatttggaga ttatggaaaa caaaagggag ataatgatgc tattataagt
    26101 caagaaaaac aacttaatat tactgccaat agtatcatta aatttttaaa gaatatatct
    26161 taaactaaaa tttaaaaaat ataattttat aagttattat ttaaagtaaa tattaataat
    26221 ataatagaat gaatatttta ttacaagggg gattttaata tggaatacga tatgccactt
    26281 tacagaccac caagtgaggc ttatagtctt ataatacaag tcacactagg atgttctcat
    26341 aacaaatgta cgttttgtag tatgtacaaa tctaaaaaat ttaaaataaa gccactagaa
    26401 gtaataaaaa atgaaattga tatatttaga agatattata agcatgtaga aagaatattc
    26461 ttagcagatg gagatgcatt gataatacca atggagaaat taagagatat tatcttatat
    26521 ataaaagaag tttttccaga gtgtgaaaga attactatgt acggctctcc taaatctata
    26581 gagaaaaaat ctgatgatga acttaaagaa ttaagaaaac ttggtgttaa aatgatttat
    26641 ctagggcttg aaagtggaag caatgaagta ttagaagata ttaagaaagg ttttagtagt
    26701 gaacaattaa taaaagttgg tagaaaagtt aaaaaaagtg ggattaaatt atcagccact
    26761 gtaatagcag gtcttggagg aactaaaaaa acacatcagc atgcagttga tacaggaaaa
    26821 atgttaggag caatttcacc agattatgtg ggtgtactta gtttaatggt agaacctaat
    26881 actgaacttt acgatttact taaggcagga gagtttacaa tactagagga taaggctgtg
    26941 ttacaagaaa ttaaagagat gataaagaat atagatgcta atgaaaaaat tgtatttaga
    27001 agtaatcatg catcaaatta tgctaattta aaaggtgtat tacctgatga taaacaacgt
    27061 ctaattgatg aaattgatta ctatctaagt aaccaaaagc taaaaagaga agaatataga
    27121 agactatagt ttttgatata tcaatctata ttgataggta aatactaaga ttatttagat
    27181 acaaaaaatc atgctacatt aatagttgct tactattgat gtagcatgat ttttttatta
    27241 aaaaacaaaa tggtatattc atatataata
//
LOCUS       DBGJFU010000043        26154 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.043, whole genome shotgun sequence.
ACCESSION   DBGJFU010000043 DBGJFU010000000
VERSION     DBGJFU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.043"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(310..807)
                     /locus_tag="ACOIAS_001793"
     CDS             complement(310..807)
                     /locus_tag="ACOIAS_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2701505.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            complement(982..1893)
                     /locus_tag="ACOIAS_001794"
     CDS             complement(982..1893)
                     /locus_tag="ACOIAS_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2701506.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            2818..3669
                     /locus_tag="ACOIAS_001795"
     CDS             2818..3669
                     /locus_tag="ACOIAS_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HGZ2701507.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            3673..4143
                     /locus_tag="ACOIAS_001796"
     CDS             3673..4143
                     /locus_tag="ACOIAS_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701508.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            4156..5466
                     /locus_tag="ACOIAS_001797"
     CDS             4156..5466
                     /locus_tag="ACOIAS_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HGZ2701509.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            5678..6424
                     /locus_tag="ACOIAS_001798"
     CDS             5678..6424
                     /locus_tag="ACOIAS_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2701510.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            6786..8327
                     /locus_tag="ACOIAS_001799"
     CDS             6786..8327
                     /locus_tag="ACOIAS_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HGZ2701511.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            8362..9468
                     /locus_tag="ACOIAS_001800"
     CDS             8362..9468
                     /locus_tag="ACOIAS_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2701512.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(9799..11085)
                     /locus_tag="ACOIAS_001801"
     CDS             complement(9799..11085)
                     /locus_tag="ACOIAS_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGZ2701513.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(11208..14306)
                     /locus_tag="ACOIAS_001802"
     CDS             complement(11208..14306)
                     /locus_tag="ACOIAS_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2701514.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSSDFEKLLY
                     QK"
     gene            15031..15183
                     /locus_tag="ACOIAS_001803"
     CDS             15031..15183
                     /locus_tag="ACOIAS_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701515.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(15476..16180)
                     /locus_tag="ACOIAS_001804"
     CDS             complement(15476..16180)
                     /locus_tag="ACOIAS_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2701516.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(16171..17778)
                     /locus_tag="ACOIAS_001805"
     CDS             complement(16171..17778)
                     /locus_tag="ACOIAS_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HGZ2701517.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(18032..19210)
                     /locus_tag="ACOIAS_001806"
     CDS             complement(18032..19210)
                     /locus_tag="ACOIAS_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701518.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(19436..19930)
                     /locus_tag="ACOIAS_001807"
     CDS             complement(19436..19930)
                     /locus_tag="ACOIAS_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGZ2701519.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(19931..20692)
                     /locus_tag="ACOIAS_001808"
     CDS             complement(19931..20692)
                     /locus_tag="ACOIAS_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGZ2701520.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            21414..24248
                     /locus_tag="ACOIAS_001809"
     CDS             21414..24248
                     /locus_tag="ACOIAS_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2701521.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            24291..25535
                     /locus_tag="ACOIAS_001810"
     CDS             24291..25535
                     /locus_tag="ACOIAS_001810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2701522.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVVKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         9137 a         4096 c         3263 g         9658 t
ORIGIN      
        1 agctttatac attatttttt atatttttca aattgtatac tcataaatcc agaagtattc
       61 taatgtgttt ataatacttt ttttcatatt ttaaattaaa taacaaatca ttatatttac
      121 aagtatcatt tatcaataaa caatattaaa tgaagaatat gtctgaagat tcaaagtgat
      181 actgataata ttaattacta aaaaagcagt aaatctttta cagatttact gcttatagtt
      241 ataaataata aaactatcta taataaatgt actatactca atacttcatt caatatagta
      301 ataacaaatc tactttattc ctatataaat atgaacttct tgtttctttg tatcaaaagt
      361 attacattga tactcttcaa aatcacttat ataagtccta tcaaaatcca tttgccaaaa
      421 ctcaaaccaa aaatctccta cttctttttg acctcctact ataacaaact ttgcatactt
      481 acctgctggt ataactttac taataatact ttctccaaca gtattattag tgtttaattc
      541 ttctaaattc ttctctttat cagaattact atttacttca caacatgcaa caaagctata
      601 cggacttgta aaatcaccct ggtaatctgt atatagacct attgtcttgt cattcttttt
      661 accctttata gcattatata tccctttgcc cataaatttt ttccacaatt cacctatatc
      721 atttaccgct tgtccatctt tatttgtagt ctccttcgat attcctataa ctattttctc
      781 atttatctct actatttcat aattcatact ttatacctca ctttgttttt attaatatat
      841 tttattttga actaattcaa ttataattta taatatacga cacctttatg tcatattttt
      901 aatataatta aactcagtga gatattatat actaaatgca agatattata tatttaataa
      961 aatctttaat ctagcattat attataatga ataaatttta gacaactccc ttagtctagt
     1021 tattacatta tccctaacat ctttaggttc aagtacttca acattgtcac caaatgataa
     1081 aatatatcca taaatccatt caccaatagg aaattctgta attacatcat atgtatcatc
     1141 ttcattttta gttattaaat ccttattaaa atcatcaaat actctaaaaa gcatcttttc
     1201 tttaaacctc aatttcaacg ttatagtatt ttccttaatt actttagaat cattcaaaca
     1261 tacttcttct attatctttc ttgaaaatac ttcatccttt atagataaat tccttatcct
     1321 actaattcta aatatcctaa aatcatcttt tagtttacaa aatccataca agtaccaagt
     1381 cttactctta tacataagct tcagtggttc gacagttctg tttgtctggc ttgacaaact
     1441 atttacataa ttaaaattta tagtattacg ccttaaaata gcttctttta tttttgtaaa
     1501 tttatcatct tcattaaaat tacttcccca ttcagtaaag tctatttcaa tccagtttga
     1561 aaaattctgc tctccaaata ttgagccaat tttatttact actgaattta tttctggata
     1621 cttagtcgcc tgtaatgttt tcagtgccac tattaaactc tctttatctt tttttgacaa
     1681 tatagccttg tctatagaat attcttctat taaagatatc cctcctcctt tgcctttcgt
     1741 catatatact ggtactcccg acatagacag agtttctata tctctatata tggttcttgt
     1801 agaaacttca aatctatctg ccaactcttt agctgttact aattttttat ttaatagaat
     1861 tactattatt tctgttaatc tatttatttt cattcattac cacctactaa aattattaaa
     1921 tttatttaat ctaaattaat catactaatt tttaaataaa taaaatctta gaatcttatc
     1981 taaattattc ttacatgatt ttatttactt tctattatct ttctcacttt ggatattatc
     2041 atcttaattt atttattact atatcaattt acttattatc atctcatttg ttatcgtctt
     2101 acttaattta cttatttcta tatcaattca cttattatta cctcacttgt tatcatctta
     2161 cttaatttac ttatttctat atcaatttac ttattatcat ctcatttgtt atcgtcttac
     2221 ttaatttact tattgctatg tcaattcatt tattattatc tcacttgtta tcgtcttatt
     2281 taatttactt atttctatat caattcactt attatcatct taattcattt gattattatc
     2341 atttacttta ttcaatttct atatcaaaaa ttagtatttg tacttaatac ttaaaattaa
     2401 tttaaagtct ttacatatta tttatttttt attgtaatat taatttccat actttgctaa
     2461 gctacttcca ctcatttatt ttaaaatttc aatttactat ttctcaattc tatattatta
     2521 tttttcaatt ttatattact aattttaaag ctttttagct attattccta caatatttaa
     2581 acaatttttt aataataaag ctatattaca atatattcaa aatatgtaat attttagata
     2641 tttttcgttg acaatgttaa atttttgttt tatccttaat taaggacatg ttattgacat
     2701 ttctgaaact tatttgaaat agttttttca ttttatttag gaaaacgtat tatacctaag
     2761 aaaacgtatt ttctcatttc agataagtaa aatatttatt cttttgggga ggaaatcatg
     2821 gaaattaata aaaaacattt tattcaagta tttttaactt gcttaatctt ggtagtaata
     2881 tgtgaattta tagggcaaag aactatagct attggaaaag cctctatagt actgttccct
     2941 atgctttatg cagttataat tggacttata ataacaccag atttattagg taaaaaaatt
     3001 aaagcactta aaaaagtggt tggagaaaaa gaaataaata ttgctggtga agttgttgga
     3061 attgcactag taatgcttgg tataaaatat ggtactacag ttggaccaaa cattgacaaa
     3121 ataattcaag ctggaccagc atttatcgct caagaatttg gtcatatact tgcaccaatc
     3181 gttgcattac ctcttgcatt aatgtttgga atgaaaagag aagctgttgg agctactgca
     3241 agtataagtc gtgaaccttc tttaggagtt atatcagaaa aatatgggat aaactcacca
     3301 gaaggtagtg gtgtacttgg tacatattta atgggaacag ttcttggtac tatatacttt
     3361 agtgtactag gttcaatatc tatttatagt ggtcttcacc catatgcttt gggaatggcc
     3421 tgtggtgttg gtagtggaag tatgatgaca gcagctgctg gttcattatc taccatggta
     3481 ccacctgaaa tggcagatac aatacttgcc tatgctgcaa caagcaatat gatgtctagt
     3541 ataacaggaa ttatattctt agtttttgta tccttaccat ttactaattt tatgtataag
     3601 attttagaac ctaaattttc gagaaataaa aaaactaaag aaattaaatc tacagaagga
     3661 gagtgctaat aaatgtttgc taatttaaat ttaaagaaaa gttcatatca attattggtt
     3721 atgtctgtta ttggagttat aattcttata ggacaaagaa ttagcgttgg tacatctata
     3781 gttacagctc ttcctggtat ggtgatgcta atattagctg ctatggcagc tatgattata
     3841 aaagatttat ttcctaaatc aatctttcca gcttttggat ttgcaactat aatcggcctt
     3901 ttacttagta tgccatatag tccaacatct gaagtattct taactaacac taataatatt
     3961 aactttatgg ctataactac tccactacta gcttttgcag gaatttctgt tggaaacaaa
     4021 atagaagctt taaaagaaat gtcatggaaa atagttataa tttcacttat tgtatttaca
     4081 actatattct ttgcatgtgc gagtatagct catatagttt taagtattca aggtaaaata
     4141 taggaggaat ttattatgaa taaagaacaa ttaaaatctc tagtaataaa aactatagat
     4201 gaaaatagag aaaaaatatt aaaaataggt caaggaatat acaaaaatcc agaatatgga
     4261 tataaagaat ttaaaactac agaagctgtt tcaaactttt ttgagcaaga attaaattta
     4321 tctgtagaaa aaaatatagc agttacagga tgcaaagcag atgcaaattc tagcaagaaa
     4381 ggtcctcata tatctatact tggagaattg gatggtatat catgtaaaga acataaagat
     4441 gctaatgaaa taggtgcttc tcatacttgt ggacataata ttcaaatagc tggaatgtta
     4501 ggtgctgctg ttggtttagt aaaaagtggc gttttagaac atttagatgg taaagtatct
     4561 tttatggcta ctcctgctga agagtttatt gagttggaat acagagagca attaaaaaaa
     4621 gaaggaaaaa taacttattt tggtggaaaa caagaattaa taaaaagagg ttattttgat
     4681 gatatagaca tatctatgat gtttcatgct tctgatttag gagaagataa ggctctagtt
     4741 ggtcctgaga gtaatggatt tgttggaaaa aaggttaaat ttataggtaa agaatctcat
     4801 gctggttctg ctccacatga tggaataaat gctttaaatg cagcaatgct tgctataaac
     4861 aacgtgaatg cacttagaga aacttttaaa gagtctgaaa gagttagatt ccaccctatc
     4921 atcacaaaag gtggagatat agtaaatgtt gttcctgctg atgttcatat ggaatcatat
     4981 gttagagcta gaactataaa tggtatgatt gatgctaatg aaagaataaa taaggctttg
     5041 atggctggtg gaatggcagt tggagctgat gttgagataa ctgaaattcc tggatatctt
     5101 ccaatattac gatacaaaag tcttgataat ttatttaaaa acaatcttga agatttaggt
     5161 ctaaaaggaa aagttattga tggtggagat tttacaggct cttttgattt tggagatgtg
     5221 tctcatataa tgcctactct tcaccctatg attggtggtg taaaaggagc tcttcataca
     5281 agagattttg aaatagttga tgaagattta gcttacataa ttcctgctaa agcaatggca
     5341 cttacagttg tagaccttct ttttgataat gctaaggatg ctaaagatat attagatacc
     5401 tttactccag ttatgactaa agaagaatat ctaacattct tagaaaaaca cgataaaact
     5461 ttataagtct atttttataa tagggtggta tctactgtaa aagttatacc actctttgtt
     5521 tttgtaacag tatacttctt ttcttttagt ttttagatta aactaagata tattatcctc
     5581 cttaaaactt ttagagctac gttgaaatat cagtagaact atgctatatt ctttctaatt
     5641 aatataaatt tctattctaa ttcaaggtgg tgaaaaaatg tttaatgcag gaaatcgaat
     5701 tataaataca tggttgtacc ctattcaaga tggctttgtc ttgattgata ctggatatga
     5761 aaatggtttt aaacatttca aaaaacgaat ttctaaattc aacataaaaa tcaaagatat
     5821 tcgatacata tttctaactc atgctcatga tgaccatgtt ggttttctaa atcagattct
     5881 agacgaatct cctaatacaa agattgttat gcatgataaa gctttagaaa ttctatataa
     5941 aggtcaaaac tcttttattg gtggttgtac cagccgtact gcctttctct tttgccaaat
     6001 tatgaaacta tttggaaaag gtgagcatag tttttctcca cttaaacaag aatttgaaaa
     6061 aaggtgtatt cttgtgtctg acaagaatcg taaagaaatt gaaaaagaac taaatggaaa
     6121 aattatagat acaccaggac atactgctga ttcaatttca ctactaattg atgaaggtgt
     6181 tttattttgt ggagattctg caatgaatgg atttccaagt actcacaaaa ttaccatatt
     6241 tgcagaaagc aaactagaat ttattcaatc ttggaaaaca atcatcaata caaaacctaa
     6301 aatgatttat cctggacatg gaaaaccatt tgaatacagt aaattagaac aaaatctaat
     6361 ctatgtaaat aaaataaatt tatatcctct acataattca aaagatattc acaggttttt
     6421 gtagaaaata caaagtaaat ttatttgatt aattaaaaaa tctaacactt tactacgcta
     6481 cttattttaa taacttaatc ttccactagc ttttcataaa attttgtttt aaaattaatt
     6541 ttaaagcgca tatgttattt aaatacatta gataaaattc gtcattaatc tacttaaagc
     6601 atatttaact aacaccactc aaccagaaaa ttcagaaaac tgcattgaca caggaaaaag
     6661 tttattttat acttttttta ggacatattg tggacacgtc ttaaatcgac aaacagaatt
     6721 taaattgtaa gattaaccac aatacttatt ttttaattat atttttaaac attagggagg
     6781 atattatgaa tgataacgta gttaagaaga aaaacttttt tgacaggaca ttaaacagaa
     6841 tagaaactgt tggtaataaa ctaccagacc cagttacaat atttttaggg ttatgtgtac
     6901 ttttacttat actatctagt ctagttggtt ctatgggtat atcagtagtt catcctggta
     6961 ctggagaaac tataaccgct gtaaatttat tgactgtaga acagcttcaa attcttttag
     7021 gaaacatagt cagcaatttc caagggtttg ctcctttggg tctagttcta gtaactatga
     7081 taggtgctgg tgtatgtgac aaaacaggac ttatgacagc aacaatcaaa gcatcagtaa
     7141 gcaagattcc tgaaacaaga gtaactcttg ttgtaatgac tataggtatg cttgccaata
     7201 tagcttcaga tgcaggtaca atattatttc caccattagc tgcacttgta tatcttggtg
     7261 ttggtagaca tccacttata ggtttatttt ctggatatgc agcagtctgt ctaggatttg
     7321 ccgcaaacat tatgataagt gtcaatgata tacttgctgc aagttttaca gttccagcag
     7381 ctcaaatgat tgattctgga tttcaagcca atgcaacaat gaacttattt tttatgatag
     7441 catcaacttt tgttctaata cttttggcaa catgggtaac agaaaaaatt gtagctccta
     7501 gatttggtaa atatgaagga aatgcagaac tagatgttga tggaagtctt agtgatgtag
     7561 aaaagaaagg gcttaaaaaa gctggtataa gtatattaat atatgtggct attatagtag
     7621 ctcttagtgt aatcggtaaa aaaccattct tagctgaccc agaaacagga gatttactat
     7681 caaacaatgc tcctcttatg aaaggtatgg tacctataat agcactagca tttttcatac
     7741 ctggtgttgt atacggaaaa actataggta aaataaagaa ggataaggat gtagtctcta
     7801 taatggctag tgcaatgagt gatatgggtg gatatataat actagcattt gctgcttcac
     7861 aatttttgca attattcaca aatagtaatt taggtctgat actagctgta aagggcggag
     7921 aatttttaaa atcagcagga ataaatggac ctttattgtt gataggattt attatattat
     7981 catgttttat aaatttattt ataggtagtg catctgcaaa gtgggctatt cttgcaccta
     8041 tatttgtacc aatgtttatg atggttggat acaatccagc acttactcag atggcttatc
     8101 gtataggtga ttgtataaca aatccactta gcccattatt tccgtacttc cctattatac
     8161 tagcattcac aagaaaatac gataaagatg ctggtatggg tactgtaatt gctaatatga
     8221 taccatactc tatatcattc ttaatagtgt ggactatact attaatattg tttatggtat
     8281 ttaatattcc actaggacct ggaatacttc cttcatatag catgtaattt atttaaatct
     8341 ttctaaaagg agaaataatt tatgttagat aaaaatagac ttataaataa ttttatggat
     8401 atggttagaa ttgatagccc atctaatcaa gaattagaaa tgtctaagtg gttggtaaac
     8461 tacttaaaag aaagaaatat agacgccatt atagatgatg caggtgaaaa atatggtggt
     8521 aatacaggta atgtcattgc ctatataaaa ggtgaagaag gaagtagacc actttgttta
     8581 tgtgcacaca tggaccaagt tcaaccttgt ttaggtgtaa aaccaatact agatggtaat
     8641 gtagttagaa gtgatggaac tactacactt ggtgcagatg ataaagctgg aatagctgct
     8701 atacttgaag ctttagaaca tgttattaca gaaaaaatac cacatagaga tatttatctt
     8761 tgttttacaa tctgtgagga agccggtatg catggtgtca aaaactttaa tcctgacaac
     8821 ttgccttgta aagacatggt gattttagat tctggtggag ctattggttc gatagcatac
     8881 aaagctcctg ctcaacaatc tataaaaatc tcttttcatg gaaagaaagc acatgctggt
     8941 atagaacctg aaaaagggtt aaatgctata ttagtagcct ctcatgcaat aagcaacatg
     9001 cacataggca gaatagattc attgactact tcaaatatag gtaaaataga aggtggtgga
     9061 gctacaaaca tagtaactga taaggttaca ttgactgctg aaattaggtc tcatatacct
     9121 gaaactttag agtatgagct taatcatatg gaaaaatgtt gtaaggatgc tgcatctaaa
     9181 tttaatacta cttatacttt tgaacatagt atgtcttacc ctagttttga acttagtaga
     9241 gatagtcatg ttttcaagtt aagtgaagaa gcaataagac aagttggtgt tgttccaaat
     9301 ccaatggtaa ttggtggtgg aagtgacgca aatatattag ctaatcttgg atataattgt
     9361 gctatattaa gtctaggtat gtatgatgta cacacagtta atgaatatgt aaatatagac
     9421 gaattatatg atactgccaa aatagtttat catatgatta aattataaat atcataatat
     9481 aactctttaa attataaaaa taaaaaccta tgtatcttca actttataag ttatatatta
     9541 aagttgtttt acataggttt tagtatttta tataataatt tgttttaaca tttttcataa
     9601 taagcttcaa tattttgcat aataatttgt tctagcattt ttaataacaa gctttagtat
     9661 tttatattaa taatttattc tagtatttta cataatcatt tgttctaatt tttttcataa
     9721 taagctttaa tactttatat agtaatttgt tgtagaattt tatacaatat aatgatttat
     9781 acagttttat aaaatatctt aaaacagtat ttcagtaata ttttttggtc ttcctccacc
     9841 aatactagtc tcttttgcac atattcgacc taaacctgca tttactattt tatttaatat
     9901 acgtcttgca cttctttcac ttacatttag atactctgct aactcttttg aatcataaat
     9961 cttacttttt ctattctcat ttatagcaat aatttttgca actgactcac aactcattcc
    10021 agtcttttta gatatttcga ttaccttttc atcagatagc atcaaagaat aatttaattc
    10081 attttcggaa gctaaaggtc ctcttatgtt ttcatcttca tctaccaaat atataccaat
    10141 ttcttttgaa tctatggagt gtcttaatgc tttatgtgca ttattttcag cctgatacgc
    10201 agttactcca gtaccaattc ctatactaag agaaaagccc atgtctttta tctctctctt
    10261 tagattgaat agcttatcat aattatactc attatcagct acccctttat gaacaaatac
    10321 aacataatca tttcttccaa gtaaaaataa ggaaccttga atacttctta catagtttac
    10381 tacaagctta tccatatctg atttttttat catgtcagaa taatagttat ctatcttctc
    10441 tttataatct attaaactca atatttcaac agcaatttga gaaaatcttg ccttacttag
    10501 agcatattca ctctttacct tgtcataact ttctttgatt aattgtatag tagcttgtaa
    10561 acgaaataca ggatagcctc tttcttttaa ttcattatat actgcaccaa aaccagttaa
    10621 cattatatcc gtcttatttt cttcaaataa ttttatatgc cattctacaa gttcagactc
    10681 tcctacttcc aaagaaaatg gattacaaaa tacactcgtt gctttaatat caaattcttt
    10741 aagtgcatct tccacaacat atctatcaac tacatctata ctgaatttat ctaattttat
    10801 atttgcactt tttattgccc aaaatgcctt cattatactc gtcccacttc tcggtacaaa
    10861 tgaatgtggc ttacttatct catacttaga ttttataaat tcataaactg cacatcctgt
    10921 aaatataata gcatcacatt cgtcttcaca tttagaaata acttctattg tatctactac
    10981 tttttctctt acatatattt tagtctttaa atcactgtct atctgtttta aatcatcctt
    11041 tatcttaaac ccagagtctg aaggacctat aatacccact atcataaact tcgctcctaa
    11101 tccataaata ttatactttt taaaaattat atcctcacta ttttctactt ctatttaatt
    11161 attttgaact actccatcct acctacattt agaatggaat aattagctta tttttgataa
    11221 agtaacttct caaaatcaga acttggaata ggcttacaat aataataacc ttgaattacc
    11281 acacaatcta aactcactaa aaattcagct tgttcttttg tctctacacc ttctgccact
    11341 acatccatat ctaaatcttt tgccatggct atagtgtgct ttaaaactat tttttctctg
    11401 tctgtgattc cttcctcatt aaagaaacca caatctatct ttaatgtatc aatattaata
    11461 ttttttaaca tatttaatga agaatatgct gaaccaaagt catccatatt taaaacaata
    11521 ccatccattc taaggtcggc tattatctta gtaaataatt ttatattttt aataaataaa
    11581 ctttctgtaa gctctaattc taaaaaagat tttggtatac tatatttatt tattaaactt
    11641 aaaacaattt ctttaaaatg tggattatgc aaatgtaatc ttgatacatt tacagagata
    11701 ggaactggct cttcacctaa atctatccat tttctaatca aagaacatac ttgttcccat
    11761 acatacatat ctaatctgat aataaaccca ttcttctcaa atagtgaaat aaatttaatt
    11821 ggaggtatta tacctttttc tggatgaacc catctaacca atgcttctgc accaacaatt
    11881 ttatttgcat taagattata cttaggttgt aaatatacat ggaattgccc attttcaagt
    11941 gcgtattcca tttccctttc catctgtttt tcttcaagtg catcatttct tatagtatca
    12001 tcataaaata tatagttgtt gatactttgt ttctttactt ttttgtgagc atatcctgcc
    12061 atttcacacg catttgtaat tggaatatga ttatccaaaa ttctataaac cccaaaacat
    12121 ggtactaaca tatattcaaa tttttttcta tttaagctat ttttaatctt ataaattact
    12181 ttatgaatat ctctatcaga gtaactctca taaaaaatgc aaaatacatc tgcatacata
    12241 cgacaacata taccattaga accaatgttt ttttgaatca actctgcaac atatattaaa
    12301 gctttatctc ctcctctaac tccataaatg tcattaatta ccttgaactt atctatatcc
    12361 atcattataa ttgcataacg cttgtcagga tatcttttaa gcattaaact tatttgttca
    12421 caaaaacttt cataattgta tagtcctgtt aatgtatctt tttggttttt atgtagttgc
    12481 ataagataat ctatattttt tatagcaact aaaatatgtg tttgtttctc acctaactta
    12541 tttttactac taatctttaa catataaatt aatgcccaat agtacttttt atctattcct
    12601 aaacgtctat actcaaaatt tagttctaaa tcgtcttgct tgctattatt aagactatca
    12661 agagatttta gaaggtggtt atcattaaat aatattatat catcaggatg aattaaatat
    12721 ttttttatgt atgaaaaagt tttttcaaga ttttccttta taggaaaact aaaaatattg
    12781 ctctctgatt gataaattgg agtaattaaa ttcgtatcaa tgcaaataga taaaattata
    12841 tcatatgtat tataagctaa tttttcataa gctttagaat acccttttat cattaaatca
    12901 tcaaaataaa ccttatcaac taactcttta tgtttaaata aaatattatt cgtcgaatca
    12961 gctaagtcac taattacttt catttcatta tttctaagtt tgttcttatt ataatacctc
    13021 ttcttatctt ggtacattct ctcatcagca actttaatta gcttatgtat atcaatatta
    13081 tcgtcactcc aaatactgcc cactgctaca ctatatatca tctcatcctt aaattcttca
    13141 cttaaccaac tcactaattc ttcaaatagt tgttttgata tatcttcaca taatacaaca
    13201 aactcatctc ctccaatacg gtaaacatta taagaatgaa agtatttttt tattattaca
    13261 catgctgaca caatagcaga atcgccatta gcatgtccaa agttatcatt aatatctttt
    13321 aacccattta tatctacaaa tacaactcct atggatttaa cttttccttt ttgaaaatta
    13381 tctatatact taatatattt atttctatta tatgcaccag tcaagaagtc atagtagctc
    13441 atataattta atttattttc tacttttctt tttttcatct cagttaataa aaaataagat
    13501 agggatgtca aaagagaggt atctccaaca gctttatgtg gattatcaac tccaataaac
    13561 cctactactt tatcatcttg ttttagtgaa gatgtcatta aactttcaat tttttgaggt
    13621 tttaaaattt catattctat tggctgttct tcttttatat tttctatatc atcaattatt
    13681 acaaaacctt tttgttcgaa taataaaaac caattcttta attcattaat atcaagattt
    13741 gttagtgcct gtttttgttc atcaacacct tcattgcacc attcatatgt attagttact
    13801 tttgttctat cttctgatac ttcaaaaaca tatgctctat cagctttatg aaatttacca
    13861 atattggtta atacaagatt tacagcatca gaaaaattag attctgttat aagatgttta
    13921 atacatccaa ctaataactc ctttatctct aatttttctg ctattgactt acttatatct
    13981 tccttttctg taatatcaag agctatttct aatcgtgcat gtttgccctc ccatttaatt
    14041 aacttgtcct ttaatataaa atgtctattt gttcgagtat tcgtattttc ccatatgtaa
    14101 tattcatctt cttttaaaat agagtttgta caaaattcgc agggagaatc tttcccctgt
    14161 aatgctttat aacattttaa atgggatata tcattaagtc ccattgcctt tttcccaatt
    14221 tcattaatat ataaaagttc atatgtatct aaagaagaaa catatacaat ttcatccaac
    14281 tcatctatta aatttattac atccatttat atatatctcc ttttattcat tttccagatt
    14341 gaatttttaa agcttttaaa attttgctat atatttggca atgatattat acttctttat
    14401 gttttaaaat agtaccttaa tgaggttcta ttttttagta aatagaatta ttattgtgtc
    14461 aatcgtacaa tagagttttg taatacttaa tttaatatat ttataattaa ctatcttatt
    14521 atgtatttac aattaattct catataaatg acaaatctcc acctcaatta agtattccaa
    14581 tcatatatga ggataatact tcattcattt aaaaatataa aactattata tattaaaatt
    14641 tcttcaataa taaattattt agattgttac tttgatatat tatataatat cacaaaggct
    14701 gatataaaaa gacaaaaagg gcaatttttt tatatcaaaa aaaatgttaa attgatatta
    14761 tccatatata aattgatatt atccatatag gtgctatatt taacaattct acttacttcc
    14821 cttacactta ttagcatata gttattaaga gtttgttgca aaacaacact tagcacactt
    14881 taaaaagtgt ggtctaataa gtaatcaacg aagaagacat aataaagcat aaaataggat
    14941 atacttttcc acgtcatcaa aattgaggat tttattatta atatatgata aaatatgctt
    15001 aataaattaa attttaatgg gagaatagtt atgaaaatat ttagtttctt gttgtttgta
    15061 gtgggaatat gtttttgtat tgctggattt atggggcatt cctcagcttt ttctggtgca
    15121 acattgagtt tttttgcaac tacaatgata gctgtaagac aagtcaaaac taagaaaaaa
    15181 tgaaataggc aaatcctaac tttataagtt aatattacat aagtaattag tctaaaatta
    15241 gatacttttt ctattacaaa aatacatcat ccactttgta ttctcttaaa aaaaataaaa
    15301 tagctatgta aattcaagat tagatagact aactctctat ccatttcatt gtaaaatata
    15361 catagctatt tgtattcgat ttaaaatact tagattttct tacataacaa ataatttcta
    15421 tctgattatt actcatgttc atttattaac cactcagcaa ctaatcattt agtatctatt
    15481 catccgccat acggtaacct accccaacct cagtcaaaat atattctggc tgagcaggat
    15541 tcttttcaat tttccttctt atacttgcca taaaaactct aagcgactga ttttcacttt
    15601 catagtaatt tccccaaact ttatctatta taaacttatg ggttaagact cttcctgaat
    15661 acttagacat caactcaata atcttatatt cgattggagt taaatgtatt tcttcaccat
    15721 taattgacac aatatgattt tcataatcta tctttaggtt tttaacttca aaagactttg
    15781 gagcatcatt atttatatta tcctgttgag ctttatttct aagagccaca cgtactctag
    15841 ccaaaagctc tgttacacta aaaggtttag ttaaataatc gtctgctcct ccatctaaag
    15901 cttccacctt atctctctca tgctctctcg ctgaaacaac aattatttta ttagtctttg
    15961 cacatgccct aactcgtcct attacctcaa tcccatcaat atctggtagt cctaaatcca
    16021 aaattatcaa atctgggtca agagaatgta taagtgatat agctgttcct ccattatctg
    16081 tctctacaca cctataattt tgagtttcaa gagatacttt tataaattta caaattggct
    16141 tatcatcttc tacaattaga actaatggct ttatttccac ttgtctttcc cctcctcatc
    16201 atacataggt atgctaaatc taaatgtagc tcctccagaa ggattattaa aggcgacaat
    16261 ttctccacca tgagcttcta ctatagattt acatatctcc aatccaagac caatacctct
    16321 tctttcaaga tttctagact tattcttagt ataaaaccta ttaaaaatat tcccgatatc
    16381 ttcatttggt attccttttc cattatctgc aacttcaaat agtacttgat ttgactcttt
    16441 ttttacagtt agttctatct tagaattttt aggtgtatgc cttatagcat tatcaattag
    16501 atttactaac acttgttcta ttaataatac atccacatga acgattatta tctcatctgg
    16561 aatatttgtt tttatgtctc tactattggc aaactttttg actcttaata tagattcagc
    16621 tattatttca tcaacaacct ctggtcgttt ttcaatatcc aattttcctt catctattct
    16681 ggtcatacta agtatattct caacagaatg agttagccaa ctcgtatctt catatatatt
    16741 tttcaataat tctactctag tttctttatc tatcacatca tcattttcaa gtattgttga
    16801 agatgaaccc aatatacttg taagtggagt tcttaaatca tgagaaattg acctaagcaa
    16861 gtttcctctt aacctctctc tctctgcctc aaggtttgct tttttatttt tctcaaataa
    16921 attttctcta tcaattgcca acgcaacctg tgttgaaact gcttctaata gtatcttttc
    16981 attttctgta ataatatcta tcttaaaaca cgcaacacct ataacaccaa gaacatttga
    17041 ttcttgacct tttataggga aataatatgc actgctgttt ttacaatatt ttgtcccaat
    17101 tccaactggg ttacctgtcc taaaagcttc ttttatagct tccttttcta atactgaatg
    17161 gaatatattc tcacttccat cattgttaca aatataaata cttggcttac ctagattatt
    17221 attctcatct gcaatagtca ctattacagt tctatttaat atatttacta aattagaccc
    17281 acaaaattca ataatttgat ttttattttt tgctttaagc aacgttttgt tattctcata
    17341 gagcaatctt atcatttttt ctctcttatg agaaagtctc acttctcttt ttatcctaga
    17401 agttaaagta cttgttataa ttgctgccat tagcataata caaaaggtca taggataatc
    17461 ttttctataa gctaaaaaag tatataatgg atgcgtaaag aaaaaattaa atgatagcac
    17521 tcctattact gaagaaagta ttccaaaaaa atatccatag gttatacttg tagcaaatag
    17581 tacacctaat atgtatacta gtataatatt agattcatta aaacctacct tttcaaaaac
    17641 tactgatatt ccagtagcaa ttgtcataac caatatagtt tttatgaagt ccttaataat
    17701 tatcttttta ctgagatagc catcttcatc ataatcatac tcttttaagt cttcatgttg
    17761 tagtcgttcc tttttcattt actacaattc ctccttattg ttactgttta aattggctct
    17821 attttatata atacaatttt atcataataa attcaatcaa tacatttaat taatgtactt
    17881 aaattactaa aaggtgctgt cttaaaatga aaataaatct ctatttcaaa acagcaccta
    17941 tatatttaaa ttactaattt agtttttaca tacttaaatc aatagtttta ttcaatttca
    18001 taaattataa tacttcaatt ataaaacatt tctatccaac aagcataaat ataaagtggg
    18061 cacatatacc tgcacataaa ccacctatag tatgcgaaag tatcgcttta ccagccaatg
    18121 gtcttgcatc taaagcatcc atcataccta tgtgtgtact taaatatcca ctccaacaca
    18181 tacccattgc tgtgaatact gctatatcat taggagttat agcgcctgat tttataaact
    18241 ctggtactaa agatattgct gctccaacag ctcctaatgc agttacaggg aatgctattg
    18301 cctctggtga tgtgaatcca aataatggtt ctattatgaa gctaatctta tcaccaatag
    18361 caggtaataa ttttatacct tcatatgcag ctcctgtgta aacagcttga cctgtagcag
    18421 ggtctgtaga tggtccaaaa gttaataaca taactaatgt acaaactact aaaactcctg
    18481 gtattatagc catacccatt tcaacaccta acttaccacc ttcaagtata gcatccaatg
    18541 ttctttggaa tacattacca tcccttattt ctctaaactc ttcaagtttt gcacctttat
    18601 catttatttg cttttcagta tcatttttag ggtcataatt ataatacttt ttagtaaaag
    18661 tcaacataat tctaacacta ataatactac ctattattgc cccaacatta cctattatag
    18721 ctggtagtac atattcttta ccttgagcta tcataaaagt tgttacaata agtcccatac
    18781 caaaggctgt tcctaaatta cataaagcag gtacttgata cttcttaaaa tattgtgtaa
    18841 atgttttgtc tttagcaaat ggtataatag ctggattatc agataaatat gttgcaacta
    18901 ctcctgctat actagcacca ggtaagcccc atataggtct cataagaccc ttaaatatct
    18961 tattaactaa agatattact ccaaactctg aaagcaatgc actaagtgca cctgcaagaa
    19021 ctgccatagc cattattaaa aatactgtgt ctaataaaag cgcatgtgct gtactcatta
    19081 ttactttaaa catcattcct gcacccatga tgcttcctac atatccaaat cctaccgcca
    19141 atagtacaat aaatacgaag gtttccatac ttactgcctt gactcttctc ttatccgaca
    19201 gcttttccat cttgaaatct tccccctttt aattttaaat taaggttcct aacattggta
    19261 caaatttttt ggtacaaact gagttctaaa acttacactc gtaatgcaag ttgtcagttt
    19321 tttgaaataa aaaaaaatta cgtttataga ataagaatat cctctttaat ttggatactc
    19381 ttattctatt ttatgttatt atattatcat ccttttgttt aaaagtcaat tttttctatt
    19441 tttgttgttt aattttattt atactctttt tatatttttc attttctttt atctttgtaa
    19501 gtactccata taagttgact tttctaaata cagtagttat aaataccaat cctaatgcaa
    19561 ttgaacttgc acacgataat gtactcaccc catattctac agcttttata taattattgg
    19621 tcaaaacaga atacatagta ttatatattc cataacctgg cactaatatt attaaacagc
    19681 aaacacttaa tgtagttgct ggagtcatat aaattcttgc acataactca caatatatcg
    19741 caaaacaaac tgtagctata aaaaaacata agttttctgt tactcccata tttaaaagaa
    19801 atttatacga aaaccaagct atacttccac cgattcctgc tagtattaag ttttttcctt
    19861 tcatattaaa tagcactcca aagcttaagg cagtaaaaaa agctgctatt acttctataa
    19921 acaaatgcac ttatatcccc cctaacttga agtacaatcc aagtaccaag aacattccta
    19981 ctgctataga tacacctaca aaaaatactt ctgctaattt tgcaagacca gaaatcaact
    20041 gtccatctaa caaatctctt aatgcattag tcaaagcaag cccgggaact aagagcatta
    20101 ttgctcctgc tattaaatgg tctaacgtct ttatgaatcc gagctttaaa attaaaattg
    20161 aacaagaagc tataataact gctccaattg catttacaaa aaagtcatta agggaagatt
    20221 tacttaaata cataataaca aacttagtca gaatcccaac agtaaatgca gctataaatt
    20281 cttccacatt tcctccaaat aatatcgaaa aagttgctgc tgcaatacct gcaaaaaata
    20341 ttgttattaa atccgagtaa tagttatctt cacttatttc atctaaaatt ttctcacata
    20401 attcaatatc taaattattt atacttattg ctcttgataa tgagttaact tcatgaacca
    20461 agtttaggtc tactcttctt gagcttattc ttttaattgc tgaatgtaat ttcccatcta
    20521 caaatacaga tgccattaca gctgttggag ttgcaaatac atctacgtgg tctattccaa
    20581 aagattgaca tattctagct atcgtttctt caactctata ggtttctcct ccactttgca
    20641 acatagcttt acctgcattt gaggaaaagt ttaaaattct atctatatcc acaaaacata
    20701 tctcccattc ttttatagta ttattcatat tttaatacaa gatattattt atttaaacta
    20761 ttttaaataa ttcatatcca acatttacat tttatgaaaa aacttgtttt ttatgcttgt
    20821 caaaatcaaa ttttaagctt gtttacacca taaaatggct gaattaatat ttgacaaaca
    20881 ctttcaacca tttatataac cttctaaatt gtataacttt ttactacaaa taaaattata
    20941 tctcttttaa ttctttctct tgagtattta gcttatagta acttcatatt tatatatgta
    21001 caatataaaa cattattttt atgatacaaa atataatttg taaatgtata tatgtattaa
    21061 ctgtgtatat ataaattatt cttaacctta tcagtttttt catatatctt catctatacc
    21121 gaccatatat taattacatt gaccatatat taataaatga tattacatag acttaagtat
    21181 attaaaaaac ttaaatttag ttatttaaca acataatact ctaatttaat ctataaaaat
    21241 ccatacctaa tataatgcaa ctcttatttt aacaccatct attacacaaa acaacatatt
    21301 aaaaagtaaa atacaaaata tctactataa accttatttt aatactgaat atatattata
    21361 atatatttaa tgtagcttaa atatagttta aacataaaga aaggaaacgg tatatgctaa
    21421 gaaaagataa agttctatta tctctacata aattatgtga aaatatgact ttaaaagacc
    21481 ttcaatcaga taaaacagga ttcactacaa tagaaattgc taaccattca tctcttgata
    21541 gaagtaatac cagtaaagaa ttaaataacc tatacaatga aaaaaaagtt ctgaaaatat
    21601 ctggtaagcc aattttattt ctacatgcta gtattataga aaatttaatt ggccgtactt
    21661 tgaacagtga tgaattttct ataaataatt gtaatgagct acttaaatca agtaaaaatg
    21721 actcagaaga tattttttca acattacttg gacatgattc tagtcttaaa ctacctatag
    21781 aaaaagctaa atctgctata ctctatcctc caaaaggtct taatacatta ctaataggac
    21841 ctactggtgt tggaaaaagt acatttgctg aaacaatgta caaatatgct gtagaaagta
    21901 aaatcttttc ttctgaaagc aaattcgtag tatttaactg tgctgaatat gcagaaaatg
    21961 ctaacttatt attaagtaat ttatttggtt atgtcaaagg ttcttttact ggtgctaata
    22021 aggataaatt cggcatagtt gcacaggctg atggaggcat attattctta gatgaaatac
    22081 acagattacc accagaaggt caagagatgc tttttctttt gatggacaaa ggcatataca
    22141 gacgattagg agaatctgac attgtgcata aagctaatgt ctttattgtg tgtgccacaa
    22201 cagaagatat acattcttct ctgcttggaa cttttttaag aagaatccct gttacaatca
    22261 acctacctgc tttacaggat aggtctttaa aagaaagatt atctcttatt aaatacttct
    22321 ttattataga atcaaaatac accaatgcaa ctataaaagt tcacaaagat gtaattaaat
    22381 cattactcct ttatgattgt tttggtaata taggtcagct taaatctgac atacaactta
    22441 tgagtgcaag agccttctta aattacaaaa ctggacttaa ggataatata gaaatagatt
    22501 tatcccttgt tcctgattac atctatattg gtcttttaaa attgaatgaa aatcgtagta
    22561 atctaaataa attatcagaa cttgattata tgctttttta tgaatttaat ggtaataact
    22621 tagaagttga atatacaaaa gaactatcta aatacaatat agatacatca aacaatggat
    22681 ttaaatactc tacagagatt ccttatctgg aaaatgatat aaggagtatg tttataaatt
    22741 acatggaaaa gtactctaca aaattacttt cgatatcttc agtaactcat tctgattata
    22801 caagtaatga gatttttaaa gtaatcaatc caaagattta ctatgctgtt gaacattctt
    22861 taaaagaggc tgaaaaaata tcgagaaaca aatacacaaa acaaacttat attgctcttt
    22921 caatgcatat aagtgcactt atagaaaata taaatcaaga cacatataat cctaagtaca
    22981 atttatataa tgataaaatt ttcttagata aagatttaaa tatagctaaa atagttttaa
    23041 gtataattga aaatgactta caaacatctt tacctaaaga tgaagtaaaa tttatatcca
    23101 tgtttctaaa tagcatgtat cttgaaccaa acaatcaatc tgaaagtata ggagtcataa
    23161 tacttgctca tggaaattca acagcaagca gtatgtgtga tgtagcaaat tcattgcttg
    23221 gtacaaacca ttgtaatgca attgacatgc ctcttgatgt aaaagtaagt accatacttg
    23281 ataaaactat agagttggta aaagaatgtg acgaaggtaa gggtgtactt cttttaacag
    23341 atatgggctc tcttatagct tttggagaac ttatttcaga aaaaacaaaa atcaaaactc
    23401 gctcaattga aatggtatct actccaatag ttttagaagc agtaagaaaa agtacactac
    23461 cagaaatgac tttggatgaa ttagctaatt ccttaagctc tttaaatcca tatattggac
    23521 gagcagcaat cgattcaaca gaaataaact atcctgaaaa gtcaagagag tatgtaataa
    23581 taactacttg tataacagga tatggttcag ctatgaaagt agcagacttt ataaattctt
    23641 cacttaaaaa tattagtaaa tacaatatta atttagttcc tcataatata gaaagtttta
    23701 aaatgtataa gaataaattt agtaatacta aaattctggc agttgttgga agtgtagatt
    23761 ttaatgtagc taacacccct tttatacaca tagaggaact actaaatgga tatggcctag
    23821 atatgctaaa caaaataata gaaggtacat tcaatgccaa tagtattaca aatataaaag
    23881 aaacaaatct atcatacaac agtttaataa taaactttat gacccaaaac ttagagcttt
    23941 tggactctgt taaattattc aactatgtca attcttcctt atttacaata atagaattaa
    24001 tagatgaaaa cttaaagagc aaatttaagc taggatatat actacattgc tcttttatga
    24061 tagaaagatg ttttaaaaat acaacttttc catataataa cattactgaa ttaattagta
    24121 aaaatcatga actatatagt ttaatacgtg aagcctttaa gccaacagaa gcttatttta
    24181 atattttgat accagacgaa gaaattgctt atataataga catgattaat gagtatctaa
    24241 ataaataaaa tttatagcac ttattttgtg catattaatt aatatatgaa atggaggacg
    24301 agattttgaa aggaaaaata aaacagttaa caatacttgc attaattttt atatttataa
    24361 ctcctgtatt tgcatttgca gatactccac cagtaccaaa ttcttcaaga gcagcactac
    24421 ttatagacca ggaaactaaa agaatattat ttgaaaaaaa cattgatgaa aaaatgccat
    24481 tagcgagttt aagtaaaatg atgacctttt tattggcaat agaagcagtt gataaaaatc
    24541 aagttaaaga gactgatatg gtgaaaatag acaagtctac agcttcagta ggaggttcta
    24601 cttgtaaact taaagatggt gatgaaatat ctctaggtga gcttatgcag ggtctaatgc
    24661 tagtatctgg aaatgatgct gctatagcca tagctaaaca tattggtaaa actgaaaaga
    24721 attttgtaaa tatgatgaac aagaaagcag aagaaatagg aatgattgat acgtattatt
    24781 ttaatccaaa tgggcttcct atatatactg acccagaaca taaagaacct cctattgaaa
    24841 atatgtcaac tgctcacgat atagttacac ttggaaagta tatgtacgac cattatgaaa
    24901 atcaagttac aagaataacc actatgcaag tatataatga tactaaaaag gattttacac
    24961 attataacac aaatcctctg cttgtttcag tacctggtgt tgatggaata aagacaggat
    25021 atacagataa tgctggatat tgccttgcat tttctatgat ggtacctaaa gatgctaaaa
    25081 acgaaagaaa tcatagattg ataggtgttg tacttggtga tggtaacaag aaaaatagaa
    25141 tttcttcttc cgctacttta ttgaaatatg gtaaagataa tttccattct aaaaagatag
    25201 cacataaggg tgatattata gagactcctt gtgttgatgg tattgatgat tttaagataa
    25261 cagttaaggt tgataaagat ttgtatggtg ttgtttcaga taacgaaaat atcaatccta
    25321 aggtagtgtt taaaaatatg aattatccta tccataaggg agatattgtt ggtgttgtaa
    25381 agtattataa tgactctggt aaatttgttg gaagtgttga tgtaaaaagt gaaagtaata
    25441 taggatgtat tcctttgaag gataagatta agattaaggt tgctaagata aataaaaaac
    25501 ttgagattaa aaattctgtt tgttttaaag catagtaaag catattatta aaagggtatg
    25561 ttaaatttat aaagactcca ctttgtggag tctttttgtt ttttacaaaa aattaaaata
    25621 gtggcaacaa ttgaggatgt gagggagagt tgccactatt ttgtataggg tattatatta
    25681 tgtttttaag ctagttttac tatcttctat gtttatataa taactcttgt gtgtgaattt
    25741 aattgataat taatatgaaa attatgtgaa gttttaagtt agttgactta gactattgta
    25801 aattttttca gaaaaattct gttcataagt ctattagacc accgaattat ttacctataa
    25861 tattccgatc aaaaaatcta ttctttttta aaagtaaggt catgattatt gtacatatat
    25921 aaaaattccg aaaattaatc tacacactag taacattaag tataataaat ttattttagt
    25981 aaaactatta aaataacctc ataagaaact atagattaaa tcaatcaatc taatctacag
    26041 tttcttgatt attccttaat atttataatt tacattattt gtttatttta aaactatctg
    26101 ttacaaatct agtcaatgtt gaaaataaat tcgctctgaa tatagtagat gatg
//
LOCUS       DBGJFU010000044        25880 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.044, whole genome shotgun sequence.
ACCESSION   DBGJFU010000044 DBGJFU010000000
VERSION     DBGJFU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.044"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..330)
                     /locus_tag="ACOIAS_001811"
     CDS             complement(<1..330)
                     /locus_tag="ACOIAS_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701523.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKS"
     gene            complement(357..1028)
                     /locus_tag="ACOIAS_001812"
     CDS             complement(357..1028)
                     /locus_tag="ACOIAS_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701524.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(1040..3616)
                     /locus_tag="ACOIAS_001813"
     CDS             complement(1040..3616)
                     /locus_tag="ACOIAS_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701525.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVILANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            3824..4759
                     /locus_tag="ACOIAS_001814"
     CDS             3824..4759
                     /locus_tag="ACOIAS_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HGZ2701526.1"
                     /translation="MANNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRVHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            4778..5515
                     /locus_tag="ACOIAS_001815"
     CDS             4778..5515
                     /locus_tag="ACOIAS_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2701527.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(5701..10758)
                     /locus_tag="ACOIAS_001816"
     CDS             complement(5701..10758)
                     /locus_tag="ACOIAS_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HGZ2701528.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(10783..11202)
                     /locus_tag="ACOIAS_001817"
     CDS             complement(10783..11202)
                     /locus_tag="ACOIAS_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701529.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(11436..11687)
                     /locus_tag="ACOIAS_001818"
     CDS             complement(11436..11687)
                     /locus_tag="ACOIAS_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701530.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            11776..12507
                     /locus_tag="ACOIAS_001819"
     CDS             11776..12507
                     /locus_tag="ACOIAS_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGZ2701531.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            12566..13477
                     /locus_tag="ACOIAS_001820"
     CDS             12566..13477
                     /locus_tag="ACOIAS_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2701532.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKFIEAGKFELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            13528..14340
                     /locus_tag="ACOIAS_001821"
     CDS             13528..14340
                     /locus_tag="ACOIAS_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGZ2701533.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            14449..15141
                     /locus_tag="ACOIAS_001822"
     CDS             14449..15141
                     /locus_tag="ACOIAS_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2701534.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            15228..16169
                     /locus_tag="ACOIAS_001823"
     CDS             15228..16169
                     /locus_tag="ACOIAS_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701535.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLNQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            16206..17363
                     /locus_tag="ACOIAS_001824"
     CDS             16206..17363
                     /locus_tag="ACOIAS_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HGZ2701536.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            17515..18075
                     /locus_tag="ACOIAS_001825"
     CDS             17515..18075
                     /locus_tag="ACOIAS_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGZ2701537.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            18103..18594
                     /locus_tag="ACOIAS_001826"
     CDS             18103..18594
                     /locus_tag="ACOIAS_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701538.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            18823..19374
                     /locus_tag="ACOIAS_001827"
     CDS             18823..19374
                     /locus_tag="ACOIAS_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2701539.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            19460..20392
                     /locus_tag="ACOIAS_001828"
     CDS             19460..20392
                     /locus_tag="ACOIAS_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGZ2701540.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            20447..21310
                     /locus_tag="ACOIAS_001829"
     CDS             20447..21310
                     /locus_tag="ACOIAS_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HGZ2701541.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            21557..23419
                     /locus_tag="ACOIAS_001830"
     CDS             21557..23419
                     /locus_tag="ACOIAS_001830"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2701542.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            23422..24888
                     /locus_tag="ACOIAS_001831"
     CDS             23422..24888
                     /locus_tag="ACOIAS_001831"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGZ2701543.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQRKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            24912..25748
                     /locus_tag="ACOIAS_001832"
     CDS             24912..25748
                     /locus_tag="ACOIAS_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2701544.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         9522 a         3090 c         4043 g         9225 t
ORIGIN      
        1 agatttattt aaaattactt tctcattttg attttttata tttattatag agtcagtata
       61 atccacttca aagttggcaa aagcagagaa taaatctata tctcttaaca aatgaattgt
      121 catacattga ttgttcttat ttagccttag aatcaatgct cttacaaatg gaaattgtat
      181 tctagaatta aataagttag aaccatcagt atctatatct aagctacgat aattaatatc
      241 tataaatata tctacatcat cattttcttc tcttattatt ctgtagtcaa aatccaagtt
      301 taaatttaaa ttacctaaaa tcaataacat ttaaatcctc ttttctataa atagttttag
      361 agtgttaatt taagctctcc atctattata gatataattc tatctgcttt tctagcaata
      421 ttttcattat gtgttattat tataagggtt tggttatatt tttttataga actttttagt
      481 aaatccatga cttcttctgt agttttactg tccaaatttc ctgttggttc atctgctagg
      541 attatacttg gttttgcaga taaagcacgt gcaatagcaa ctctttgctg ttgaccacca
      601 gataattggt tgggaagatg atttttcctt tcattaagac ccaataaatt tataaggtca
      661 tcaatatatt ctttatctat ttttttattg tctaacttta gtggtaattc tatattttcc
      721 tttacattta atacaggaat taagttatac tgttgaaaga caaatcctac cttttgtctt
      781 ctatatcttg ctaattcttt atctgaaaga caacatatat ctttaccatc tacctttata
      841 gtaccattac ttggtctttc aagaccacct actaaatgta gaaatgtact tttcccactt
      901 ccacttggtc caattatagc aacaaattcg cctttattta tttttaaatt tgcattcttt
      961 agtgcatgta cagatgtttc ctttttccca taaatcttac ctaaattaat agtttccaat
     1021 ataggcataa ttatccctcc tattctgtaa tgtttatagc ttcaactata ctaattttat
     1081 ttatctttct tgatggaata tatgtagcta gaataccaac aattatattt gctaaaataa
     1141 ctaggatata tattttgtaa tctattgaaa atcccaatcc atatgataca aagccctgca
     1201 tataatacat tttaaattgt attataattc ctgacacgat agtaattata cttgagagta
     1261 caccatataa tattccttca taaagtatca tatttttaaa gcctctctca gagataccaa
     1321 tagctcttaa cattccaaat tcactagttc ttgaagttaa gttataactt atgttgttta
     1381 ttatgttaag tacactgatt atgaacatta ttgctactat accataacta taaatcatag
     1441 ttctagatgt catcttatca gaattttctt tttctaccat catatctact gttgtagttc
     1501 caggaacttc acttccaatt ttacctaaaa ggttatttat ttttttcttg tttgcatttt
     1561 cattaaggtc tgcataaact aagttatact tatctacacc agttaatttt tttaggtatt
     1621 cgctactcgt tattacatct acaccatcat ctccagagta cataccatta tctgcaaaag
     1681 gataacttat aatagctcca actttaaatt catattctaa gtattcataa ttatctttta
     1741 gactttcata aagccctgta tccatcccac cctttggata tttgactttt atagtatcac
     1801 ctacttttat atctacagct ggtgttcctt caccaggagt aaaagtcttt gtgtatttct
     1861 gtgttttaga aatttctggt acatatacaa tagctaaatt ttcttttttc atttttttca
     1921 agtttatatt tccacttact aagtattttt ctaatgactt tatcatctca tcattataac
     1981 cttttagctt ttgctttagt gtatatttgc cagtagtctt attttttatg aacagaggat
     2041 ttagcttata ataaggagtt gaatttatag cttcgtagta atctaatcta tttattttat
     2101 ttacctctat tcttgtgttt aagatactag cagttttgac tttgtcaatt ccatctatat
     2161 tttttatttt atctatctga ttatcattaa acccttttat atctttaaaa gaagtattgt
     2221 taccaatttc attgagataa acatttgata gaataaaatc tgcattcata tatgtttgtt
     2281 cttgagtaga tttgttttgc acatctaaaa aagaatattt ataattgtct tttatgacga
     2341 ttatacctcc tatactcata gataatacaa ttataatgaa ccccttttta tttctaaaga
     2401 tgtttttcgc agatatagat tttgttactg acatatttaa agttaatttt gatattaaat
     2461 aattaacttt ggttacattt ttatcagctc caaaagtttt tctgatagca tcaataggag
     2521 aaattcttct tatttttaag tatgtaaaga aacttataat tagtattatt agtattgtac
     2581 atgcaattga taagagaatt attttatctg gaataacaaa aggtgtaaca tttatatttc
     2641 cttcaaattg aatatttcct acagacctat taaatatttg tgcacctccc atacctatac
     2701 atatacctat aggcattgct attagagcaa gtatgaaaag ttcataaagc ataagtttga
     2761 aaatcctaaa gttggtggaa ccaatagctc ttaagacgcc atattcctgt actctttggt
     2821 atattgatat aacatagata ctatatatta caataccagc aaatagactc atagcaattg
     2881 caacgtattt acttgaatta tctaaagtac cgtttttcat agtagattct ataagcattg
     2941 agtttcttcc tatacttttc tcatctaaca tagcattttt tattatattg tctatgtttg
     3001 tatttatatc actgccttca ttaaattcaa tataagttgt aaaatcattc agttctgatt
     3061 cattgagatt agaaaaaatt tcacattgtc cctttttttt ctctataaat ctatcttcaa
     3121 ggattccaac aactttaaaa gtttctggat tttctttttg atataattta aaagtaaggt
     3181 tttgatttat ctctggtttt aagcccatac tatttaaaat ccatttctcg actaccacct
     3241 cattgctagc ttttggaaat cgacctttta taagtttaga atgactagtc aaataatttt
     3301 tatttgcata tagtatgttt ataggtaatt tttcattaac atctgtagac gcatagtaag
     3361 atgttatacc cagattttta atatctttgc tagattttat ggactttaat tgatttttct
     3421 ttatattttt aaagtataca tggtcagttc caagctctct tttcaatcta tctacatctg
     3481 cttgctgagc acttcttgag agggtaccaa taccaacaac gagtgatgta gcaagaatta
     3541 tactcacaac aattgctaaa gacctagctt tataactttt tatatacctt agtactattt
     3601 tcaaattatc tttcatattt accacttcct ttcttaacta aatcaattat atatttatct
     3661 aaaatatata gcaaagtaag tattttcaag ttttaagtag ttaattgtac aaattcatct
     3721 gtatttttac aagtttaatt aatctatatg taaattttaa ctttttacat aattttttta
     3781 aaatgatata atccctatat aaattgtaga ggggagggaa atcatggcta acaattttgt
     3841 atttcctata gtaatactaa ctttgtttat atttagcaat cttataatga ataagtataa
     3901 attttacaaa gaattagaca tattaaaaag aggcatatta acatttttta tatcgtgttt
     3961 tgcattgatt ttatttatgg agggcactaa atatatacaa ttaaggttag gatatagttt
     4021 tttacttgaa ggagtattaa atattatact ttttttggtt ttaattattt taagttttta
     4081 tacaggcttt aaagttataa gattgataaa taataataat gaacaggaat ataaactaca
     4141 aaaactagat agtaaaataa atcataagga taatataatt atcaaattaa gaagtcaaaa
     4201 acatgactat ttgaaacatt tgcaagtggt gtattctcta ttaaatactg ggctagatga
     4261 agatgccaaa gaatatataa tgaatatatc aaatagtttt aaatcaaaaa attcaaggta
     4321 tggaaaagta tcttatttag atgctatagc ttcattaaaa tacgaggaat gtcttgaaaa
     4381 ggatatatct tttgatgtct acattcaaga ttttatggat aatttagaga ttgaacctaa
     4441 tgatatgagt tcagtactat taaacataat agataatgca atagagtctt tgtctactat
     4501 aaaaaaagaa aagaagtata taagagtaca tgcatatgaa gatgaatttc agtatattat
     4561 agctataaaa aataatggtc ctaaaataca taatgttgaa agtatatttt ggaatggatt
     4621 tagtactaaa gaaggacaag ataggggata tgggctacat ttagttaaaa atatattaga
     4681 taagtatgat tatagtatac ttgtaaatag tgatgatttt ttaactgaat ttgtaatctt
     4741 aatcccaaaa tattattagt taggaggaga aaagtctatg ataaaagttt tgatttgtga
     4801 tgatgataag attgtaagag atgatatagc aaatatagct aaagagtctg gatatgtaga
     4861 ttatgttaaa aaagttaagg atggtataga ggcaattgaa tttataaaaa atgagaaaat
     4921 agatttattg attattgaca ttgatatgcc atataaaaat gggattgatt ctgctgaaga
     4981 aattctttct atagataagg atatacatat agtatttgtg acgggtttcg ctgattatag
     5041 tcttgaaagc tttaaagtgc atcctataga ttttgtagtt aaaccattta aaaaagaaaa
     5101 aatacttgat tcaattaaca tagcaataga ccatattaac tcccataaga ttgctaataa
     5161 taattttata gaagacaccc tttttgtata taaaataagg aagcagatac atatgattaa
     5221 ttttgatgat atagttatgt ttgagaaaaa ttcaagagca attaatctac acactaaaga
     5281 tgaagatata ataaagtttt atgagaactt tgatgattta aaaaaacgcc tacctcctaa
     5341 tttctttatg acccatagac aatacatagt aaatctaaga tttatacata aggttatacc
     5401 tataaataaa tcttcattag agataaggtt tataaatttt caagaaaatg ccatattaaa
     5461 caaatctcta gaaaaagatt ttttatatag attttatagg gttaaaagat tctagtgaag
     5521 atagaagaaa aatacttttt gtgtgatata ataagtacca taaagataat gtataagaga
     5581 agtttatata aaaattattt gtaattatga tttaatctat ataaattaca gtatagtata
     5641 tattggactt tctaattttt tttaagaaag tccaatatat tttagggata gtttttatac
     5701 ttataaatta cttaagatac tagggtcttt aatcttacta tcttcagcaa gtaatagtat
     5761 cttagacata atctcagcag ttttagggtc ttcatcagcg aatggaagga atatcttacc
     5821 tctactttga gaatgtatag gaagtatatt caaagcaccc atcccttttt tatgtacaat
     5881 accagaaccc atatgcacag aatactcacc taaattacct gctataagag catgagagcc
     5941 ttcaaaattg acattgttaa tttttaataa taataaaagt tctttagcaa ttgctatacg
     6001 catctctaca gtagaatggc tagtttcagg gtcaacacca ccagcatgag cgacacttac
     6061 aacaaggtct atatcacgca taacttctga gaatagaatt ggaggtatag aagctaattc
     6121 taaactttta taagtttttc tatcaaaaaa ttctactgtt tcaatagtag gaggctctat
     6181 atctgctggt gaaaaccaat ctgcaagtgc aaacatcttt gccactatat tttctttata
     6241 aaatactttc tgtaatccct cttcataatc gacagtccaa agctgatttc taagaagtgc
     6301 tacagttttc tttggctgaa tttggtgacc agcatatctg cgagatatag ttttttcaga
     6361 taattcatca ctgtttattc tataatactc acggaatact tgcttaaatg gttgaacaat
     6421 ttgcttacta aataaatcat gttggaactc actccactta ccagattcaa acaaatgaac
     6481 tggatgtgca agttttaatt tattttttgc cgtcaactta taagtttcac cttctatatt
     6541 gcaaataaag aacttagact tatcattata aacatatcca atattatcat cacataccca
     6601 tactagattt ttaatcaaag ggcagataac tggattttta tagatattgg caatttcttt
     6661 aactgtaaat actatttcat ttttcatagc ttgttctaaa ctctgttttg cacgagaata
     6721 ctgactcttt aaatcttttt ggacagattt aagttcaagt acatatggat gtttagcaat
     6781 agcttttggt atactcttta aagacttgtt gcctttttga actttagtag atgcttttcc
     6841 gttttcatca acatctatcc aaattgaagt accatcaata tcttttggtt taaatagatg
     6901 agcaacttca tttagttttt cagtctcaag agcccacatc atacgattta catctccaaa
     6961 tccagcactt ctagctagat tttctagcgc tatagtacaa gcttttgatt cactttcacg
     7021 acgttgtgca ccaaattgct tgctttcttt agcaaacttt tgaataaatt cataacggaa
     7081 agttgcttct tttaggtctc cttttttcat aggaagtaaa gagaaacttc tcaatttatc
     7141 ttgatttcta tttgtttcaa tttctttcat caacatgtct ttatcaagct taccaagtgt
     7201 agcatcagca tagagttgtg cacgtctatg agagttacca ccactagtta tatatttagc
     7261 tgacttgtaa agtatattga atctttcttc cccaatagtt ttatatgaat ctaaaaacca
     7321 atctacatca aatgcaccat caatgaaact acgtggtgaa ataggggaga atagagcaac
     7381 ctctgattct ttttgtgcag agaagccttc atttatatgt gcatggaagt accaaattgt
     7441 acttttcaga cccttccagt ttaaatattt ttcaattatg tttacccatt gaggggcata
     7501 catagcagat tctatcaatc ttaactcagt aatgtcggta tttttaagta aactttttaa
     7561 tttttttgag tcatcttctt tagtaggata gcaattttta agtagagaac ttaaaacatc
     7621 tttttttgaa taattgctac catatgaata tcctctctgg aacgtttctt taccaagagc
     7681 gaccaaaata tttacaaaat aatttatacc ttcaaagtat tgtatattat tagctagatg
     7741 agacacctct gttggcatat cacctctacg tgattcaatt tctataattc ggtttgtaac
     7801 agtttttaaa ttatctttaa gccaagggta cttttctaat gtgtcgccag cccatctagg
     7861 atttgtcaat gttcttatta tatttgatgc gttgtatcca cttatcattt ctctattaac
     7921 ttgctcttca gtcattaatc ctaattcatg tgcacgagca aacacattta tgctagtata
     7981 gaaaggaaac ttatattcta gcaactgata atttgaaaac gtagtgtaga aaaacttagt
     8041 aaaagaaata tcatcataaa taaacgaatg tgctttatca agccaatagt ttaaaatagg
     8101 ggcatttaaa gcagggcagt attcattttg tctgtaatag tttttagtaa attcaactct
     8161 ttgtttttta cattgttcta tagtcaataa gtttctaagt ttatagtata tttctaaaca
     8221 atagttaaat atttctttat ggtcaaactc atttagtaaa actcttaaaa gttttcttgt
     8281 aagtccatta aatttaagtt tttcaagttt tgtatatatt ttattttgtt ttttagagtc
     8341 aaggacattt gaatatatag atgaaaacca acttttttct gtttcattat atccacatgt
     8401 acttatacta taaaataaaa tatcaagtat taatttgtaa tctaagtcaa gctttttact
     8461 agtttctctc caaagctctg ctaatggata attatcaaga gtatcattag aaactttata
     8521 tttaatagga gtcaagttat aagtagaata acctaaaagt actttgtcta cattacctga
     8581 ccatctttca acttcatatt cataatctgc attttcagta ataagctcat caaatttttc
     8641 ataaactttt attattttat ttatatccgt atcgagaatt ttttttgctt ctttttttgt
     8701 tgctacagaa acagaatcct cacgagtaga taaacttata tttgagataa ttttattaag
     8761 ctttgatgag ctattagaat catctttaaa atcaaattgt tctggaattt gaactttgtt
     8821 ttcagggtta tatattccaa aaccattttc tttgcagtaa tcattttttg ctttacctaa
     8881 caaattatca attagtattt gaacttgaga tgtattcttt ttagatttat caatatcttc
     8941 tataagtaaa acaagcttat tatatagttc ttgtctagca gtattgtctt ttaataagtt
     9001 tacaatttca agacctccaa ggcatttttg agaatttgaa gattttatca gtgaagaagc
     9061 aatattttcc aaatttgaat catcttgttc aagtaaaatt tttattactg tttgtctaat
     9121 agagcttgtc tttaaagaca atagattggt tatcttatct aattctacat ctgttagctc
     9181 aagctttcta agtagactca ataccgtttc tctattggaa acactcttat cacttaaaca
     9241 ttccaataaa aattcacgtt gtctatctgt ctttggttca tttacaaaat tgactataag
     9301 tgaagctctt aaatctgaac tcataagatg acaattttcc ataagcacat ctataaattg
     9361 agcattcata tcgtatgctg ttaaagtcat catacattta agtatttcat gagaagaaat
     9421 ttcaatccaa gtccatggaa atacacttcc tgaaaatgat tttgtctttg aatttaaaat
     9481 ttctaaagta tgttttaaac tttcaaattg ttcttttcta gcatcaaagt ctttgtcaat
     9541 aggagtgttt gacaagtcaa aattagtttc tctgttttgc caactatagt agtagccaac
     9601 agaaccttct acatagaaat ttgtaattac ccatgctagt atctctaaat catcaccctt
     9661 aatgttttta gatgctatag aatgtttaaa atatgtactt tctgtatgcg ataaaaagta
     9721 aagagctaca agttttttat atttttcctg acaagaaagt aagttttcaa taagagagta
     9781 agagtcatat atgttgtaag ttgcagtagc ccataaggca aaataaattt caagattatc
     9841 attgctatta attgattcta tacggaactt ttcatcagtc aatgatttat atgaaagttc
     9901 taaacattta cgtattattc caggtttttg gtctgaaaaa ccaagtcctg tccatgtatc
     9961 caaggctctt ataacagaac taaatcgctc taaattgttg tccaaaatag tttttaacat
    10021 gtatattgat gcagaaattg taccctcatc taaactctct acaatttgct gtctaagtcc
    10081 ttcttgttgt ttggctgcca aaagaagttt agaaagcatt tcatagcatt tatcactatg
    10141 actcttcatg ataccaatta tcatttgtct tgtaagtaga gcagtttgat tttctccaaa
    10201 tattacttct tctaaagcac ttattacttt ttgatttcct ttgtctattt ctagtgctat
    10261 tttatcactc attatagagt catagtacaa gaccttattt ggaagagtaa gataatctat
    10321 aactgaaaaa tcataataaa ttaaatcaac atagtttgaa ataaacctta taatacctct
    10381 aaaataatcc acaagtttat ttgagcgata ccctttacga taatatcctc ctgaatatgt
    10441 ataatccatc tttcttttaa ttaattggca aagttcttct acttgttcag aagtaaatat
    10501 taattctaat gcatttttta aaggtccaga aaggagctcg tccatatttt taatttcgta
    10561 gtagtggcaa ttgtagtatt tctttatcat atttaagaat ttgtcttcag gttctctata
    10621 atagtatgaa ctattttcta tgcagtccca aagagacttt gctaagtcgt agtactgttt
    10681 tgaagaagat ttagtaaaat tatttttaaa attttcaatt attttaggat ttttttctac
    10741 tctagtacgt tctaacattt atgtacctcc aaattattta ttttaaaaca ttcttccagc
    10801 aagcaaggta agtcttataa aagtaaatat atctccactt ttcagattta tagaggaatc
    10861 gagtttttca ataacatctt catttataag cactttgtag attccatctt caaatgcctg
    10921 taacgctacc tctattgcct tttctacaga ttgtttattc tcattgtaaa tagagccaaa
    10981 tccaactttt ccaatatcag cttgagattc aatttcttca ttagttaaga agtttattat
    11041 tgatttttct gggattttct cattatattc aagaacattt ttagatacaa tttcagagat
    11101 agcttctttt aggcaattga tgttgtcaga tacatcaaaa ggggtaagtt ctagcatagg
    11161 tttgcgtttg ccaacagcct taactctaaa ataaatttgc ataaaatcac ctcgcaaaat
    11221 attaattatg tatttagaaa tttaaaaatt tatctatttg ttatattata tcattttgtg
    11281 aattattaat gtgaattatg gaattatttt acaaaaaata gaaaaaaagc tatcttaaag
    11341 tctaaaaaag caataaaaga taacttttaa ctataattat atagaattat aactgtacat
    11401 ttattaaatt ttaattttag aattattttt gataactatc tttttttatt tataaatttt
    11461 gctcctaaat attttttgat atcttcttca ggagaaaagt catcaataag tatttctttc
    11521 ccagtgtctt caaatactac aagaacttca cagtcacctt gtaagaaaaa ttttctaact
    11581 gtatcttctt taacttctct aacttttatt acattctttt tttctttttc aaagtctttt
    11641 gtgtaataac ttcccatcat attaattatt tttgtatatc tattcataaa atacctccat
    11701 atgcgattaa atttaaaaaa tatgttataa taattgtttg tattaactta ataattataa
    11761 caaggagtaa taataatgac tagtataaaa aataaagata ttttaaattg tatagattat
    11821 tccattaaaa ataagctctt tgacaaacta aatgatgtat ataagtcctt gccagtagga
    11881 aattgttctg gatgtggaaa ttgttgtatg gagtcagtag gtataaattt aattgagttt
    11941 ttgaatatat tttgttatct tgaagataga gttgatttaa aaaaaagctg tataagtaaa
    12001 atattagatt attattttga ggagtataca agaaagaacc catgtccatt taaagatact
    12061 aataatagat gtttaatata tgaagttaga ccattaaatt gtaggatgtt tggacattgg
    12121 aaaaaagagg attataataa aaatttagat aatgttattg agaaaaataa taactataga
    12181 gagttaatga aaaagcaata tggatttgaa ataaatgatg aagtagtaaa ttacaggata
    12241 gattattgtg atagatttat tccaaataaa gattatttaa gtaaatctaa gagattaagc
    12301 ttttttgatg agcttatgat attagactca aatatttatt caagtggaaa tatagatata
    12361 gactttaggg atagaggtat agttgaatat tttatagagt cattacttta tagaaattta
    12421 gcatataatg tgaaaataag gatatctaaa gaatacaaaa taagaaaaag aactatagat
    12481 agaataaaaa ggattgttct tgtatagttt tatattagta agtaataaat tgtatcataa
    12541 atattgtcaa aatgaggtga agaaggtgaa tcttacaaaa gtaaaaggaa ataccttttt
    12601 tataaaaggt ggcacgaata caggaatata tgtatttgaa gataacacag cacttatgat
    12661 agacccaggg ttatgtggtt caagacctag gagagtaaat aaagtattag atgaaaataa
    12721 tatacaggtt agatatatta taaatactca tggtcatgat gaccactatg gagcttgtaa
    12781 tcaatttaaa gaatattata aagatgtgtg tataatgtca tctgaatacg ataaattata
    12841 tatagaaaat ccagaattgt tttcaaagta tattgttggg ggaaagagca atgtatttat
    12901 ggataatatc ttaaaaaata aaatgttgga tgatataaaa atagataaat ttatagaagc
    12961 tggaaaattt gaattaaaca atgaaatgtt tgatataatt gaatttcagg gtcatacacc
    13021 tggtagtata gctgttttga cgaaggataa tgtgattttt gttggcgatt tattgatagg
    13081 agatgaaatt ttatcaaaat acgatttctt gtttctatat gatatacaag aacaaataaa
    13141 ttcattagaa aaattaaaaa atatagattt tgagtactta gttttagggc atggcaagca
    13201 agttatatcg aaagaagatt cttataaatt aatagaaaaa catttgaatg ctttaaaaaa
    13261 atatttatat caaataagag agttgcttgt agaaccgaaa tctttagaaa ttttattaaa
    13321 aaacattata aataacaata atttaagcaa taattataaa gagtatcatt tttttaaatc
    13381 ttcgttagta tctgtcataa gttacttagt tgatttagaa gaaatagggt atatattgga
    13441 aaatggagaa ttgctttact atacaaaaac aaaataaatt atgataaaat aaagaaggtc
    13501 ataaaaaaca aataaagggt gagcaatatg gaatacgaaa aatggaatga gatactagca
    13561 ccttatgaac atgcagtaga ggaattaaaa ataaaattta aaaatataag aaaagaatat
    13621 ttagcaaaag gtgaatattc tcccatagag tttgtcacag gtagaacaaa aaaaatatct
    13681 tccataatat ctaagttaaa aaggataaat gcaaaagata tagaaactga aattgatgat
    13741 attgctggta taagaataat gtgccagttt gttgaggata tatatgctat agttgatttg
    13801 ataaaagtaa gaaatgatat gactataata ggagaaaaag attatattac aaattacaaa
    13861 gatagtggtt atagaagcta tcatgttata ataaaatacc ctataaactc cattgcgggt
    13921 tcaaaggaaa ttatatgtga aatacaaata agaactcttg caatgaattt ctgggctaca
    13981 atagaacatt cattaaaata taaatatgac cattacatac ctgagactct tgctgaaagg
    14041 cttagaagag catctgatgc tgcctttctt ttagaccaag aaatgagtga aataagagag
    14101 gacataatga aagctcaggc tatgtaccaa atgaagtcta tagctattag agatactcta
    14161 aatagaatac aagagttata tgacttgggt gatacacata aagctatgca gtaccaaaga
    14221 aaacttgata gaactgaaac agataaaaat ataactgaaa ttttggaatt aaaacaagaa
    14281 atagatatat tattagagca atataaggat actaaggtag atgataaagt agttcagtaa
    14341 aaactacttg tagaaaattt gctaaatatt aatggggctg cctcttaatg gaaaataatt
    14401 tctaaaaaga gacagcctat ttagtgtaat taagaaagga atgatattat gagtttgtat
    14461 gctataggtg atttacattt ttcaacatca gtcaataagc caatgaatat ctttggaagt
    14521 aattgggatg gtcatgaaaa gaaaattata gataattgga aagaagttgt taaggaagaa
    14581 gatatggtct tagtactagg agatacatct tggggaatta atttatccga agctaaaaaa
    14641 gatttagata taataagcaa acttccaggt cagaaaatac ttataaaggg aaatcatgat
    14701 tactggtgga caaccgttac aagtttaaat aagctatatg aagacatgcg ttttattcag
    14761 acaaattttt atgaatataa agattatgca atttgtggag gtaggggctg gatatgtcct
    14821 aatgatgtta aatttgatga gactgatgaa aaagtttata aaagagaaga acatagactt
    14881 agactatcat tagaatctgc tcgtaaaagt ggtcactcaa aaataattgt gattactcat
    14941 tatcctccta cgaatgataa attggaagaa tctttattta caaagttatt tgaagaatat
    15001 aatgttgaaa aggtgattta tggtcacttg catggtaaag aatcttttaa aatgggattg
    15061 aaggggattc gaaatggagt agaatataca ttagcatctt gtgactatac agaatttaat
    15121 ttaataaaag ttcacgatta aaaaacataa attggtaaat actataagat acatgattaa
    15181 aaataaatat ttgagggatt tcggttttga gaaaggagac acgacttatg aggtggatat
    15241 tagtttttat atttttatat cttattcctt tagtagtgat attcagaaat tacaagaatt
    15301 ttaagaggtc ttgtatctac agtagtattt atgtggtatt agctacaaca ataatgataa
    15361 gtaatattta tatgagtgga cttaataaaa taaaagagtc tatgtattat cacaattata
    15421 tttctgatga gacatatgat gaaaagtatg tatcaaattt taaggaaaaa gaaagtactg
    15481 atttaaacca ggataataaa aagaatgaag aaatatcaaa taataaaaat ttaaacaaca
    15541 aacaagatag aaatatacaa aataaagaaa gtaatgaaga aggaagtaat gatataaaag
    15601 aaaagaataa ttctgatagt aatgtttcag tgaataataa atcagaaaaa caagaagata
    15661 atacaaaaga aaataataaa tatacaagta aagaaaaaaa tagtgaagat ataaaaaaaa
    15721 tagatatgga aagtatagga atttttaaga aagaaattta taatatagaa agagcagctt
    15781 tagttccaat gagagaatgc ataccttaca ctaaggatat agcaaaaaat ataactaaat
    15841 tatctagtat aaaaaaagat gttacaaatg ccagaaacaa gtgtaaagaa gttgtaaaag
    15901 aatatgaaaa tatgcagatt ccagaacttt ctaaggagaa gtatacagaa acattgagta
    15961 atgctagaga tgacgtaaag aaagcatatg agcttagaga aaaatctatg gaaagtgctt
    16021 tgaaattaat aaatactaaa agccctaaat atatagggaa gataacagaa tatcttgatt
    16081 tatcagataa gcagatagaa agttttaaaa atagattgaa cgatttaaaa gaagaaataa
    16141 ataattcaga aacaaataat attaattaat aagatttaag gatataagtt aaaagggaga
    16201 ttatgatgaa taaacagagt ataggaaaac taaatatgtt ttttatgggg attactcaaa
    16261 gaaaccttga taatagggat ttttttattg agttagaggt gatttttaaa tctggaagaa
    16321 aagagtttaa aggaattgca atagaggaag ataataaata taaatttaat tttaaaggaa
    16381 aatcagattt atatacgttt aatgagttgc ttaagaatat ttctaaagag gcagaaaatt
    16441 atgatggatt agtatttaaa tatgttgagc gaggaactat tgttgtaatt gaaggagaca
    16501 ataagaaggt aaatgtaaaa tatttagata ataaggaaga agtacctaaa atagatgaat
    16561 ttacggcatc tcaaataaaa aacagagatt attatgtaaa agtaggacaa gcaaatgcac
    16621 ttttaaaaga aattggagta cttacaaaag atggtaagat aaaaaatgat aaaataagaa
    16681 aatacaatca gatagaccat tttgttgaat tgatagatag tattttaaaa gaaataaagg
    16741 ataaagactg tataactatc ttggattgtg cttgtggaaa atcctactta agctttgtct
    16801 taaattttta tataaaagaa gtattgaaga aaaactgtta ttttatagga atagattatt
    16861 cagatgtagt tatagaagca tcaaaaaata tggctaaaaa cttgggttat aaaaatatgt
    16921 catttataaa agaggatttg actaattata cacctaatag ggatgttgac cttgtaataa
    16981 gtctacatgc ttgtgatacg gctacagata tggcaatagg tctaggaatt agagcaaaat
    17041 ctgaagctat agtagtagta ccatgttgtc ataaggagct attagggcaa tatagatatg
    17101 aagctatgga accaatatta aaacatggtg tatttaaagc tagatttgca gatttaataa
    17161 ctgatggttt aagaacattg ttattggagg gcaatggata tgatacttct gtagtagaat
    17221 acatatctcc acttgataca ccaaaaaatt taatgataag agcgataaaa actaaaacga
    17281 ataatgacaa agcattaaaa gaatataaag aactgaaatc acaatttgga gtggaaccaa
    17341 ccttagaaaa acttatatat tagttaaaaa tgtaaaattt acgaaaagct tgcttaaata
    17401 tgcaagcttt ttggtattat ataggtatag tcatatccag agttttgatt tataattaaa
    17461 gctgtgctat aatatttgat taatcaaatc aataaatgtt acatggaggt aaaaatgaac
    17521 aaattgaata tatgtattga tatagatgga acaataacaa gcccttatca ttttcttcca
    17581 tacctaaatg atatctttaa taaaaatata actgaagcag agtgtataac acataattgg
    17641 gaagaattgt atggttcggg aataagagat gtgtatgcgg agtttaataa taagtatata
    17701 cactcatatg atgaagcaaa gattgtagag ggtgctacag aggtaattag cacattatct
    17761 aaaggacata atttatcttt tgtaacggct agacatgagt gtttaactga tgtaactaaa
    17821 aattggctat cgagacaagg attttcagat atagatgttt acttattagg aagtgattat
    17881 aaagttgaaa aggcaagaga actgagttgt gacatattta tagaagataa tcctttaaac
    17941 tcagtgcaac tagctgatga tggtgttaaa gtaatacttc tggatactaa ctataataaa
    18001 gatgttaaac atcaaaatat agttagagtt agtaattgga tagatattga aaagattata
    18061 aaacaaggta tttaaagatt tgtagattga aagggggagc atatgataga tttaaaaatt
    18121 gaaaatagaa aaggaaaaat ccatgtaaag agcagtgaaa taaaagacat tttagaatta
    18181 cgtccagact tcgaatatgt acaagatata tcaaatacta taaatcaaga aaatatattg
    18241 gtatttgatt gccagttgac aggaaattct tttgatatgg atgatttaga tatagaagaa
    18301 atattggaag agttaggtga agaaatagat gagtcttatt tcagtgtttt atttgaagat
    18361 gtaagagcgt atttaaaaga tgctactgat gagatagaag cagacttaca ggataattac
    18421 ttatttgata atttgagatg ttattttgat atatacaata taaatcaaga atttacagat
    18481 tttaagtttg tatttttagt ttcttttaaa gatataaaaa tttcttcatt agcaaaccta
    18541 gctaagattg tttctaagag acagcttata ggtgcatcta agttttatag ttaaaatgaa
    18601 agattattat aataaaagct agtaaccttg taaaaatata ggttactagt tttttattta
    18661 ttcattttca caaaatttca gaaaactatt tacattctaa aaaaagtaat gtatattaaa
    18721 cataatgtac attataatgg ataaaaagtt tctattaaat tactaagttt atttaataat
    18781 caaaataatt tatcttatat tagtatgtat tagggggtat ttatgcaaag tttaaatgaa
    18841 ataattgctg aaaatttaaa aaagataagg aaagaaaaac atcttagttt agacaagatt
    18901 gcacaactta gtggtgtaag taagagtatg ctatctcaaa ttgaaaagtg tgaggtaaat
    18961 cctactatat ctactttgaa gaaaataact aatgggctaa aaatctcatt tacatcattg
    19021 atggagaggc aggaaagtga catagaattg attcaaaaaa gtgatataga ccattttatt
    19081 gaagataatg gaaagtatat aagttatcca atatttccat ttgactccaa aagaagattt
    19141 gagatattta tgattgaaat agaagaaggt ggaaatttag attcaaatgc tgaacttcca
    19201 ggaacacaag agtttataac tgtttttagt ggcgaagtaa ccattaaaat aaatggagaa
    19261 gattacattg tatctagtgg aaattctata agatttaaag ctgatgtatc acatatatat
    19321 agaaactcag gcaaaggtat agctaaaatg agtatggtag tatattatat ataaataatt
    19381 taatggaatt agagtgatat ttagatgatt ttaggtatga agatatatta tatttaagtt
    19441 ataggagtgt gatttatgga tgaataataa aaaagcagtt tttttctttt gcagtgattt
    19501 aaagaaagac ccagtagcca gtaatgtgat aaaatgttgt gaagaaataa tggatttaaa
    19561 tcaaactgat attgttattg atgataattt tgtattagag tttaaggata aaaacaataa
    19621 cttatttcat tttgtaaaaa caaaagatgt aataagtcat aattataaat attatcttcc
    19681 aattttaaat agacatttta gagattatga ttttgcagga gttgtaaatt ggcatgaggg
    19741 agaaaatgct ccagaacata tcctaacagc tcatacaatt ggagatgttc caacaggaga
    19801 atttggaaat tcaaatccaa gatactttaa aaatctaatc aatgctattg aggatataaa
    19861 aaatgaaaat ctacttgatg aatttactac attaacagaa gcaactcact ggtctggaac
    19921 aacatatggt gaagaatcta aattaattac agaatattct gtacctatgt tggatattga
    19981 gataggaagt agtagtgata gttttaataa tagtatagca atacaggtat tggcaaaatc
    20041 attaattaga gtatttgatt gtgatgaacc tttgaagact ttgttatgtg ttggaggggt
    20101 acattttgag aaatctttct cagatattat aaaaaataaa gaatataaca tatcaattgg
    20161 acatgtattg ccaaatcaat ggattgtaag tggaatgtat gatgatgaat ctggatttga
    20221 gaaactagaa aagtgtataa atagtattga aggaggaatt gattgtatag tttttcatga
    20281 taaattaaaa ggtacctata aagagcaatg tcgtaaacta ggtgaaaaac ttaatgtacc
    20341 tgtatttaaa cataaaatat taaaaaatcc aaaagactta cctatatggt agttttatac
    20401 agttaatata acaataagtc ggattaattt ttaagcgagg tcaaaaatga tattatctta
    20461 ttttgcatta ttaattagag tagtgctatt agcttttgaa agagtagttg taagattatt
    20521 aggtgatgaa gaaggagata tttataaaaa tatagcttca tcatttctat ttttctttat
    20581 agggggagta tgtttacttc catttagcat aatggataga gttagtgatt ggtcgttttt
    20641 aataccatgt tatataagta gtttagtcta ttcaattggt tcagtagcgt atgtaacgtc
    20701 tttagcaact ggggaagttt cacttgttac tccaataaat agtttaaatt cattgttttt
    20761 actgctttta tcagttatat ttttaggtga aggtttaagt ttagcaaaag ttgcaggaat
    20821 cataattatg attggtggag tgtttatatt aaaaaaagta agctcttttt taaagaatgt
    20881 ctctgtaatt tttaacaatt taccatgtag attaatgttt ttatacatat ttttgcagtc
    20941 agtaggaaga gtattggata aggctttttt tgtacaagta tctcctatat tatattcaac
    21001 tatactgtat tttttcgtag gtttaaattt atttatcttt ttagcattta aaaagaaaca
    21061 aaaaataatt tgtgagattt ttagtaataa aaaggggctt tcaattttaa gtggtgctat
    21121 taatggatat tcttatttag cattattaat tgctttaaat aatctagaac ttagtatagc
    21181 agagccattt tctcaagtat ctatgataat aactttaata cttgctcatt ttatattcaa
    21241 agaaaatata aaagaaaaaa taccaggttc aattttgata ctaattggtg ggtggctact
    21301 gttactttaa ttgatataaa aaatgaatta taaaggtgct attagttagt atcagaaaat
    21361 ttaaaaaagt attgacaatt cttttaggca aatgttaata taataataca aaatagtata
    21421 aatagaagac atgttactgg taatgcaggc aggatcgaat atattgcttt gatttttcaa
    21481 ggtgtttata taggttctgc cttttttgca tcttgaatgg ccattcataa aaaattatgg
    21541 agaaggagat tacaagatga attataaaaa aactgccatt gacattattg aacaagttgg
    21601 tggttcaaaa aatattttag ctttagaaca ctgttcaact agactacgct ttaagttgat
    21661 tgatagaagt aaagtcaatc aggatgcttt aaaaaaagta caaggtgtta tgagtatagt
    21721 taatggggca caatttcaag ttgtcattgg aaatagtgtt gttgatgtgt atgatgaaat
    21781 tttaaaagtt tgccctttag atacaaatga tagtactgta aaaactgaag aaaaaataaa
    21841 cttaggaaca agatttatag aatttattat taatattttt caaccactga tttttgctat
    21901 tgcaggagct ggtattttaa aatcactgtt gatgttatta gcaatgttta atattttaaa
    21961 atcagattct actatatata cattattagt tgctatttca gatgcaacat tctatttcct
    22021 acctcttatg gtagctgtaa ctactgccaa tgtattgaaa tgtaatcgtt tagtagctat
    22081 tgccgctgta ggatacttat tgcttccagc aacaacaact gctttaagtg aaggtgtcga
    22141 actatttgga tttacaattc caaacattgc ctataatgca caggttttcc cggctatctt
    22201 atgtgtgtct ttcttggcaa caatggagaa aatttttaac aagtattcac caaaaccaat
    22261 tcgaactttc tttgttccta tgatgtcttt ggcaattact gttccaatta ctctaactat
    22321 tttaggtcct ttaggatata acgttggtac aatttttaca accattattt tattcttata
    22381 taacaaaatg ggattcttag ttgtaggatt acttgctgct tttcttccat ttatggttgc
    22441 aacaggaatg cacaaagcac taatacctta ttcaattaat acaattggaa agttgggtta
    22501 tgaagcttta tatatgacat cttcattagc ccacaatatt tctgaaagtg gtgcttgttt
    22561 tgcagtagcc ttgcgaacaa aagatgaaac attaaaacaa acagcattat ctgcaggaat
    22621 ttctgccttg atgggaatta cagagccagc tttatatggt attactttac aacataaaag
    22681 agcaatgtta ggtgttgtat tctcaagtgc aatttctggt acatttttag gattatttgc
    22741 agtaaaagga tttgtaattg ttggaccagg gcttgccagt atgagtatgt ttattgaccc
    22801 aaacaatgga aacaatttga tttttgcaag tattggtttt gttattgcaa tattaggctc
    22861 gtttattatt acactgatta tttggaagga agatacaaat acagtagagt catcaattgg
    22921 agatgttgtt gaagaagagc tagaagaaga aaaagaagta aatatcttag taactccagt
    22981 agaaggaaaa gtaatagatt taagtaaggt taatgatgaa ctatttgcaa gtaagacttt
    23041 gggagatgga gttgcaatta taccgaccaa tggaaattta tacgcacctt gtgattcaga
    23101 agttgtgatg ttatttgaaa caaaacatgc aattggttta agaacaaaaa atggggctga
    23161 gattttgatt catattggaa taaatactgt aagtatgaat ggagatggat ttaaaacatt
    23221 tgttaagaca ggcgataatg taaaagaagg agatttatta attcagtttg atttagacaa
    23281 aatctcacat gctaatttag attcaactgt tatgattgta aacaataatg gagcagatta
    23341 tgcttataaa gtgttaaacc agtcttatgg caatgtaaaa aaaggaagta tcttgtttga
    23401 tgtaaaggga ggaatataaa tatgagaaag tttccagaag gatttctttg gggaggagct
    23461 acagctgcta atcaatttga aggtggttgg aatttaggtg gtaaagggtg gtctgtatca
    23521 gatgtagcaa aggcacactt tgatgcagat gtgaaagatt ataaatctaa taatgaaatc
    23581 acaacaaaag atatagaaga aggattagca catcctgaag atgaagtaaa ttatccaaaa
    23641 cgtcatggta gtgattttta tcatcactat aaagaagata ttgctctaat ggcagaaatg
    23701 ggatttaaga catatcgaat gagtattgct tggtctcgta tttttccaaa tggagatgat
    23761 aaagagccaa atgaagaagg tttacagttt tatgatgatg tatttgatga gttgataagt
    23821 tatggaattg aaccacttgt tactatgtca cattatgaac caccattaaa tatcgtttta
    23881 aactatgatg gatggtattc acgacaagtt atcaatatgt ttgttaggta tgtagaaact
    23941 atctgtgaaa gatataaaaa caaagtgaaa tattggctta catttaatga agttgattct
    24001 atgattcgtc atccgtacac tactggtggt ttagttcgag accgttttaa ggataaaaac
    24061 tttgaggaag ttatttttca agctatgcat catcaatttg tagctagtgc ccttgcaaca
    24121 aaaatttgtc atgaaattat tccaaattca aaagtagggt gtatgttaac aaaattaacg
    24181 tattatccat acacatgtag accagaagat gttttggcaa ctcaacgaaa aatgagaagt
    24241 atttatgcat atagtgatac gcaggttttt ggtgagtatc cagtatattt actgtcgtat
    24301 tttaaaaata ataacattca aatagtaaaa gaagaacatg atgatgaaat tatgaaaaag
    24361 tatccagtag attttatttc attctcttac tatatgtctt cttgtgaggc agctgataca
    24421 actggattag atattacacc tgggaatacg ttattagctg taaaaaatcc ttatttagaa
    24481 atgagtgaat ggggttggca aattgattca ataggtctac gtatatcttt gattgaatta
    24541 tatgatagat acagaaagcc tttatttata gttgaaaatg gattgggagc caaagatatt
    24601 ttaactgaag ataaaaaagt tcatgaccaa tatagaatta attatttaaa agaacacttt
    24661 aaatgtatgt tagatgcgat tattgaagat ggtgttgaac tttggggata tacttcatgg
    24721 ggttgtattg atttggtcag tgaatcaacg aaacaaatgt caaaacgtta tggatatatt
    24781 tatgtggatg ctgatgatta tggaaagggg acatataatc gatataagaa agatagtttt
    24841 tattggtata aaaaggtaat tgaaagtaat agtatagatt ttaaatgacc aatagaaggt
    24901 gagcaaataa aatgaactac ataataaaga aagttttgaa ctcgagcgtt gttttagttc
    24961 atgacacaaa ggataatgaa tttattttgc tagggaaagg aattggatat ggtaaaaaaa
    25021 caggagaata tatttgtggt tccaatgata atcaaatgtt tgtccctgtt gaaaatacaa
    25081 aatcaaaaca atttttagaa ttaatggatg atattcctgt cgacatttta aagataacac
    25141 aggaaataat agtagaggca gaaaagttat tgaactcatc ttttaataaa aacttatact
    25201 taatattagc agaccatttt aactttgcaa ttgaaaggat gcgtaaagga ataaaaatta
    25261 caaatcgagt attttgggaa ataaaaaatt attatccaaa tgagtttaag gttggaatgg
    25321 ttgcaattta tatggtagag gagagattgg gcattcaatt accagaggaa gaagcagcta
    25381 acattgcttt ccattttgca aatgctatgg cttcagatgg aaattcatat gatacgatta
    25441 agtatgccaa agtaattgga gaaataatta atatttttgt tttttctttg aatagaacct
    25501 tagataaaaa gagtatgcat tatatgagat ttataacaca tataaaattt tttgtggaaa
    25561 gatttttttc agatagtatg ttaagcagtg gtgatgattt attatttaat caaatgaaaa
    25621 ggtcaaatcc aaaagaaatg ttaattgcat taaaagttag agatttttta gaaaagaaat
    25681 atggaaaaaa gttaacaaat gaggaaatag ctttcttggt tgtccatatc gcacgagtta
    25741 agcaatagag tgaattacta atattatctt atacgtctac atggtagaaa attgaaagaa
    25801 ggtaaagtat ataaagaaac tgagacatat tattttgttg ttcattatgt ttattttatg
    25861 agtgtatcct gttaaaaatt
//
LOCUS       DBGJFU010000045        25469 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.045, whole genome shotgun sequence.
ACCESSION   DBGJFU010000045 DBGJFU010000000
VERSION     DBGJFU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.045"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            130..1095
                     /locus_tag="ACOIAS_001833"
     CDS             130..1095
                     /locus_tag="ACOIAS_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGZ2701545.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWSKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(1293..3404)
                     /locus_tag="ACOIAS_001834"
     CDS             complement(1293..3404)
                     /locus_tag="ACOIAS_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HGZ2701546.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(3706..4410)
                     /locus_tag="ACOIAS_001835"
     CDS             complement(3706..4410)
                     /locus_tag="ACOIAS_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HGZ2701547.1"
                     /translation="MNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMSA
                     TVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDYL
                     TIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRVS
                     RSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMAP
                     GTNYARRLITATRVLQ"
     gene            complement(4612..6525)
                     /locus_tag="ACOIAS_001836"
     CDS             complement(4612..6525)
                     /locus_tag="ACOIAS_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGZ2701548.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKHN
                     VDEIIFSIANIEKRRKKEIIDVCKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(6609..7700)
                     /locus_tag="ACOIAS_001837"
     CDS             complement(6609..7700)
                     /locus_tag="ACOIAS_001837"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2701549.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(7706..9073)
                     /locus_tag="ACOIAS_001838"
     CDS             complement(7706..9073)
                     /locus_tag="ACOIAS_001838"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HGZ2701550.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKVVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            complement(9027..9779)
                     /locus_tag="ACOIAS_001839"
     CDS             complement(9027..9779)
                     /locus_tag="ACOIAS_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2701551.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(9827..10735)
                     /locus_tag="ACOIAS_001840"
     CDS             complement(9827..10735)
                     /locus_tag="ACOIAS_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2701552.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(10757..11506)
                     /locus_tag="ACOIAS_001841"
     CDS             complement(10757..11506)
                     /locus_tag="ACOIAS_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2701553.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(11535..12710)
                     /locus_tag="ACOIAS_001842"
     CDS             complement(11535..12710)
                     /locus_tag="ACOIAS_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HGZ2701554.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(12725..13564)
                     /locus_tag="ACOIAS_001843"
     CDS             complement(12725..13564)
                     /locus_tag="ACOIAS_001843"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2701555.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(13567..14724)
                     /locus_tag="ACOIAS_001844"
     CDS             complement(13567..14724)
                     /locus_tag="ACOIAS_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HGZ2701556.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(14740..15462)
                     /locus_tag="ACOIAS_001845"
     CDS             complement(14740..15462)
                     /locus_tag="ACOIAS_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2701557.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(15474..16547)
                     /locus_tag="ACOIAS_001846"
     CDS             complement(15474..16547)
                     /locus_tag="ACOIAS_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HGZ2701558.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(16569..18275)
                     /locus_tag="ACOIAS_001847"
     CDS             complement(16569..18275)
                     /locus_tag="ACOIAS_001847"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGZ2701559.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(18321..19877)
                     /gene="murJ"
                     /locus_tag="ACOIAS_001848"
     CDS             complement(18321..19877)
                     /gene="murJ"
                     /locus_tag="ACOIAS_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGZ2701560.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(19992..21047)
                     /locus_tag="ACOIAS_001849"
     CDS             complement(19992..21047)
                     /locus_tag="ACOIAS_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HGZ2701561.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(21142..21828)
                     /locus_tag="ACOIAS_001850"
     CDS             complement(21142..21828)
                     /locus_tag="ACOIAS_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGZ2701562.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(22048..24072)
                     /locus_tag="ACOIAS_001851"
     CDS             complement(22048..24072)
                     /locus_tag="ACOIAS_001851"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2701563.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(24326..>25469)
                     /locus_tag="ACOIAS_001852"
     CDS             complement(24326..>25469)
                     /locus_tag="ACOIAS_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HGZ2701564.1"
                     /translation="ISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQ
                     LRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILF
                     GFFLNYLGNPDLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLCIATF
                     IIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCT
                     MGCVLLKENLGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGI
                     LLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTL
                     VEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
BASE COUNT         7945 a         4639 c         2761 g        10124 t
ORIGIN      
        1 ttcttataaa ttatatcatt gtcttttttt tgtaactgaa acaagtattg aatatatctc
       61 aatactttaa tataattaag tgtatgctat taaaaaatat acaggaaaaa gaagaaagaa
      121 ggggtacttt tgtcaaaatt aaagaaattt gttatactac tggctttctt ggtagtaata
      181 ttccctataa gtgtctatgg atatttttat tataaattat ctgctattca tgactcgagc
      241 ataagtagtg atttattgga taacaatgac cataaaaatg aagatgggat aattaacata
      301 ctacttatgg gaacagatgc tagaccaaat gaagattctt caaggtcaga tgctatgatg
      361 atacttacta ttgataacaa acacaatgat atcaaactta catcattagc tcgtgatagc
      421 tatgtagaca tccctggtca tggtaaacaa aaacttactc atgcttatgc ttatggacaa
      481 gctgatttat taatacaaac aattgaagag aattttaata ttgatattca aaattatgct
      541 tgtgttaatt tcgaatcatt tatgtatata attgatgcta ttggtggcgt tgaggttact
      601 gttgaaaaag gagaaatacg agaactaaat aaatttattc ctgaaactta taaatggagt
      661 aaaagtgatg ataaaggtag catacaatac attagaaact ctggaaagca gactctaaat
      721 ggatatcagg cactatcttt tgcacgtatc agacacaacg atacagcctt tgctagagat
      781 ggcagacaga gacagattat acaagctata atcaagaaaa cggaaacttt acctgttact
      841 aaatatccag gtttattaga tgcagttctt ccatatgtta aaactaacat gaagcctaat
      901 gcaatacttt ctttaggagc tcaagtttta aaaatgggtg acttaaatat taagcaattt
      961 gaatttccta ttgatgatga aattcatagt acaggtggta tatatggaaa agctggatgg
     1021 gttcttagat ttgacccaga tactttagac atattacacg attttatatt taatgacata
     1081 gagtttaaac aataaaaaat tcaacataaa gtttattaaa aagtataaga ttaattacta
     1141 aaaaagaact atatcgcata attattttca agttgctgtg tacacttgat tacaaaatgc
     1201 atatagttct tttttatttt ctaatttatc atgataattt tcattttaat ctacatttgt
     1261 aaaatactct gtattttaaa ttaacttttt tattacttaa ctaagtttaa gaaagtgttt
     1321 aatgtgtttt gatttatacc atctccagct tcgtatacta gagtagtttt cttaggctct
     1381 aaagcagttt tctgagcaga tgttacacta tttccaagca taactactgg tgattttaat
     1441 ttagccgcta aaggacctga tgttaatgca tctaccaatt gattactttt acttacaaac
     1501 attgatgaat attcagcatt tggatagaat ttttgtatta cttttccatt tgtttcttct
     1561 ctattttctc cagctattct atttttagat acatcatttg taatgacctt atccagctta
     1621 tttattactg aatcagatat acttagatta ccacctataa aataagcatc ttttactttt
     1681 aaatctgata tccaattgta aacttcatca cttacattat cttttgatac taatactatt
     1741 ggttgctttt cttctcctgc ttttgaagct atagaaagag aatctgcttc acctgtacca
     1801 cttgttatat atagtttttc tactggatta gtttttacta attcttttgc tatcattaaa
     1861 gatgtactat atctatctga accaccaact ctatttattg atacatttga caatgtatct
     1921 tttatctcac tctcaacttt atctccaata gagttatttc ctccaattaa aataacttta
     1981 cttggattaa gtctttttaa ctcattctta gtggatgtag gtatattatc cttatttacc
     2041 aatagtatag gagcatcatt tgatgtagca agtggtgttg atgttatacc atcaactata
     2101 gagtacccat ttactaatac tactgtatct gaatttgtag cccaaccttt tttacttatt
     2161 gctactgctg tgtcatatct ttcgtcgcct cttaatttct ccactttaac attagatggt
     2221 ggagtaactg gttcttcact actagaatat aaactcttta aatccttttg tatagaagta
     2281 ctatttgcta tatctttagc tgagaaatac atactacctt ttatttcact atactttcta
     2341 tttaatgtta cttgttgtac tatttctttt gctatatttt gtccaccata actcgattga
     2401 ccttgcttat atataccttg tcctatgtat aagtctacat ttgttccttt tacttcatta
     2461 gcccaccatg ccactaattt agaataatct gctgctttaa gaccaattgg ccagtaaagt
     2521 tgtggaacta catagtcaat caatccttgt tttatccatg ctcttgtatc tgcatatgta
     2581 cctacataac tttcattacc tgatgtatct gaacctgttg ggtcactact cttatttctc
     2641 catatacctg ctgggcttac accaaataca acattaggct ttatactctt tatagacgct
     2701 tttacatctc taagtagagt atttacattt tctcttctcc aatcatcctt attttgtcct
     2761 tttccatatt tttggtatgt tgcagtatca ttgaaactta ctcctggata gaagtaatca
     2821 tcaaaatgaa ctccatctat atcgtaattt tgaactactt ctgctatact atccactatg
     2881 tactttctaa cttctggtaa acctgggtcg tagtaatact tatttccatg tttaactacc
     2941 cagcttggat ttttctttgc tggatggttt gctggcaatt tatctatgct ctcatctgcc
     3001 atagttattc tatatggatt aaaccatgca tgaaattcca ttcctctttt atgtgcttct
     3061 tctattaaaa atggtaatgg gtcatatcca gggtctttac cttgtgtacc ggtaagatat
     3121 tcagaccatg ggtttatatt tgatttatat aaagcatctg attttggtct tacttgaacc
     3181 actgctgtat ttattcctac actttttaat ttgtccaata aatctgtgta ttcctttttt
     3241 tgcttggctt cattattctt agtcttaggc cagtctaaat tataaactgt agatatccaa
     3301 gcagctctca tttctttatc atttgatgaa tcagcaaatg aacttacact acacatagta
     3361 agtgtcatta tcaatgataa tactaatata cttatttttt tcatttcatc ccccttaatt
     3421 ttttaaattt attttttcta caatatactt cttattaagt atatctaaaa ttcatattat
     3481 ctgtcaaata ttgaataata tagttaaaat ttatatttga tatttgatta taatatgaaa
     3541 taatttatta taaaaaaaac agcaaagaaa tttatcttct ttgctgtttt tataacgttc
     3601 atcactattt taatattact aaactcttaa atctctatgt aaaacattaa atgtaattat
     3661 atatttaatg ttaatattca atcctatgta aatcaatatc tattattatt gtaaaacacg
     3721 tgtagcagtt ataagtcttc ttgcataatt agttcctgga gccatgctag taacttttac
     3781 tacatctcct gtttgtggtg aatgtatgaa tttagaatct cctacataca atccaacgtg
     3841 atttattgaa ccacctgaac caaagaaaac taaatctcct ggttttaatt ctgctcttga
     3901 tactttcttt cctactgttg cttgtgatct tgatactctt ggtaaattaa ctcctgctcc
     3961 atttttatag acataagaag tgaacccaga acaatcaaaa gcatttggtc cagttgctcc
     4021 ccatctatat ggcttacctt gttgcttaaa tgctaagttt aaaacatttt gtacctttgc
     4081 atctacattt ccaccttgaa tagttaaata atctccattt acccatcctg taacattgtc
     4141 aacacttttt attttaatcc atccatttga atgtaattct aaaacttcaa ctttgtctcc
     4201 tgtgtttatc tttcctacta ctgcatcatt tgcactagct ccacttctta catttaaaaa
     4261 atcacactgg tctactgttg cactcatagg tgaattagtt ggctctgctg cactagccgt
     4321 tattgctcct gcttctactg tgacaactcc tgctattaac accatagcta taaatttttt
     4381 caatatatgt tttttatttt taatattcat cataatctca aatacctaat cctttcgaaa
     4441 atttaacatt ttgttccttc gtttccaatt cctaattaac attctatctt acatcaaaat
     4501 aaattgcaac cttttttgta atctttttgt aatctttttg taatcttttg tttcttttta
     4561 gtatatattt gtttcttttt gctccccctg gttacaaatt agaaatatat tctacttttc
     4621 tctagttgtt gcaatttctt tgatattagc atcttctggt ttaatataag ttggcactaa
     4681 ttccctcata ataccatcta ttttttcaac ctcttcattg ttagtaattt ctcttaaaag
     4741 ttttaagtat tttgttattt tttctctatc aacatctatt ggtctaccaa taaaaatctt
     4801 tttatgctct gtatccaaca gaccttcttc tgacataagt agttcttcat ataacttttc
     4861 tcctggtcta agaccagaaa attcaatttt tatatcaaca tctggctcaa atccactgaa
     4921 tttaattaag ttttttgcta aatccaatat tcttacaggt tctcccatat caagtacaaa
     4981 tatttctcca cctttagcca tagccccagc ttgtatgact aagcctactg cttctggtat
     5041 tgtcataaaa tatctaataa tatctggatg agtaactgtc actggtccgc catcttcaat
     5101 ttgcttctta aaaagtggaa ttacactacc attactacca agcacatttc caaatctaac
     5161 agcaacaaat tcagtctggc tttcagcatt catagtctgt ataatcattt cagcagctct
     5221 tttagtagct cccattatgt tagtagggtt tacagcctta tctgtagata taaggacaaa
     5281 tttcttagct ctatattttg aagacaagcc agctatgttt aaagttccaa atatattatt
     5341 ctttatagct tcgccagggc tcgattccat aagaggaaca tgtttatgtg ctgctgcatg
     5401 aaataccact tctggtttat atttattaaa tatttcatcc attctttttt cttctcttat
     5461 tgatgctatt actgtcttta aatctaattt atttttatat tttctaagta attcttgttg
     5521 tattgagtaa gcattatttt cataattatc aactataata agctctttag gattaaatcc
     5581 tgcaatctgt ctacaaagct ctgaaccaat tgagcctcct cctccagtta ttaggattac
     5641 ttttccctca atgtaattac ttatctctct taaatttgtt tttataggct ctctacccaa
     5701 caagtcttct atctctactt ctctaatctg ttttatatca acttttccat ctataatttc
     5761 gtaaattcct ggtattgtct ttattttaca gttagtgttt ttacatacat caataatctc
     5821 tttttttcta cgtttctcta tgtttgctat tgaaaagatt atctcatcta cattgtgctt
     5881 ttctaccaca tcttttatat ctgaagttgt tcctacaatt ttcacattat ggattattct
     5941 tccaatttta tttttatcat catcaattat agccactgct actttcttaa gttctggatt
     6001 tctttttagt tcctgtataa ccatttctcc tgcatctcct gcaccaatta taaggatatt
     6061 tgaaaccttt cctctagaat atgtcttgtt catgactatt cttatagctc tataggctat
     6121 tcttgcgcca cttgtaaaca ttataataaa tattgtattt aatacataaa acattatagg
     6181 ataatcaaac cctaataatt tatttattat ataaatcgga attaaataag ctaaagttgc
     6241 cacaaaaata gatataattt cttcttctcc tgcatatctc cataatgtgt tgtagcactt
     6301 aaacaatcta aatgatacta tatgaagtac tatatatata cacactgaaa ttattatatc
     6361 attagtggta aatgcattta aattacctgt caagcttaaa gacattcgaa acgctactag
     6421 tacgctacaa atatctagaa ttaatagtgt taaatgtcta aatttatatt ttttaaaaag
     6481 cctattaaaa aagctttctt tctctaaatc tatactttct cgcataacta gaagccctcc
     6541 tttaaacagc aaaagctacg cacctaagca tagccaacaa catctatctt ttgctgtatt
     6601 ttttcatttt attttttata tttatcaaat atagaatcat aaattttttc tatatcatta
     6661 aagtttaaat taacctcata attttctcta acaaattgac gccctttctt acccatatta
     6721 agtctaagtt catcattttc cattagtttt attatagcat tttttatttc ttctggttgt
     6781 tttggttcta caattaagcc agtttcattt tcccatacag attctggatg tcctcctaca
     6841 tttgtaacta taacaggtac ttcacatgct tctgattcta tagctgccac tccaaatccc
     6901 tctcttaaag aaggaaatac agttacatca aaagaattaa aagtcttgct tacattttct
     6961 ggagatattc ttcctaaaaa ctgaacatca ttttctatac ccaattcttt agctaaattt
     7021 attaggttgt ccatctgact tccagagcca cctattttaa gaattatttt cttatcttta
     7081 tattcatctt taagcatctt aaatgcttgt attaaatatt ctattccata ttttttttct
     7141 aaactcttta taattcctat tacaaaagcg tcttcttttt ctacttccat aggcttaaac
     7201 ctatccatat ctactccaaa aaaggttaca tctacatgtt tagctgtata tttattagcc
     7261 tctttagcca tatctctact atttgaaaag atatagtctg cttttctaaa gttatgcttt
     7321 ataatttgtt tttgtataaa accattatta gggaaatcat aaatatcagt accccaaaca
     7381 gaaataatat atggatgata cccaagtaga cttcctataa atccataact acttgcatac
     7441 tgtgcatgca atatatctgg ctttatttca tttactagct tctttatctg ctttattgaa
     7501 cctaagtatc ccgttttttt agaaatattt tcatttttta gttcttctac attagaacca
     7561 aaattataaa tttttattcc ttccatgtga cctccattta aagagattac atgaatgtca
     7621 tatcctttat ttttaaaaaa actacaccat tttaaagtat gaggatttga cccatcagct
     7681 aaataacaaa cagtcttcat aatatttacc ttcctatcga ataatactca attcctaact
     7741 cttccatatc tttaatttta taacaatttc ttccatcaag gactaaagga tttttcatct
     7801 tttccttata cttatgtata tcataattta atacatcttt ccattctgtc attataaaac
     7861 atatctctgc atcttctatc gcatcatcta cagaatcagc ataaagtata gagtcaccaa
     7921 atattttctt agtattattt acaccaattg ggtcgtatac agtaactctt gcccctcttt
     7981 cgagcaaata ttttatattt ggtatagatg gtgcttctct tatatcatca gtgtctggct
     8041 taaaagtcaa acctaaaaca gcaaccttca tatcttgaaa gctctctact aacttagatg
     8101 cctttctaac taatttaagt ttttgacttt cattaacttc tattgcagct tttactgttt
     8161 ttaattcata accattatgt tcagataacc agtgaagagc ttttgtgtct tttggaaagc
     8221 aagaaccacc atatcctatt cccgccttta aaaacttact tcctattcta tcatcataac
     8281 tcattcctaa agctacatct tctatgttag ctcctgctat ttcacaaaga tttgcaatat
     8341 cattcataaa tgatatttta agagctaaga agttatttga tgcgtatttt atcatctcag
     8401 cactatttct atttgtaact attataggtt gattaaaagg cttatagatt tcctctaata
     8461 tttctctagc ccattcactt tctacaccta taacgattct tgaagcatgt agagtatctt
     8521 ttactgctgt tccttgtgat aaaaactctg gattagatgc aagttctact tttacatcat
     8581 tttttaggtt tctatttata taatcttcta tctcttcatt tgtaccaatt ggaactgtag
     8641 attttattac tactagagta tcttttgtca cagtttcagc tatttgcttt gcaactacct
     8701 ttatataatc taggttggct gaaccatcac ttctctcagg tgtacctaca gctattaata
     8761 ttacatctac atcttcataa gcttttttat agtctgtagt gtaatctatt cttcctctgt
     8821 tgtaattttt aaccataagt tcttctaggc cttcttcata tataggtgct attccaagtt
     8881 tcattgattt aactttttgt tcatctacat caacacagac tacattttca ttgcctacct
     8941 ctgctagaca aacacctgta accaaaccta catatcccgt cccagctata gctattttca
     9001 tttttttacc tccaagtata taaaacttat actctttttt taatggcatt aaaaacatag
     9061 aaacaaaata catatagact ttttggtaaa cttaaatttt caacatttct ataagtattc
     9121 caagttcgtt ttaatgcttt tatcttgtta cttgatatag aatttccaac taatctgtaa
     9181 ttcactaaat cttcttgaat cccatatgca tatttttcct ttttaagtaa ctgtaaccag
     9241 gttgcagtat cttgtcctct tcttaccaaa ggcatgctga actctcctac aatctcctta
     9301 tcaatgacca ctgtagaaca tcctattata gtatttctaa gtaatccttc atagtcaatt
     9361 ttcttaggag ctcttgcaac cttattcgtt tttgacccat cttgtctcat atatctataa
     9421 gatgtaaaag aaaaacctac attttctttt aacatatatt ctatctgaat ctctaatttt
     9481 ctactatccc ataaatcatc actatctaca aatgcaataa atcgaccttc cgcattctta
     9541 ataccaacat ttctagatac tgcagctcca gaattttttt caagctttat atattttatt
     9601 ctagcatctt cttttcgaaa actttttata atttcacttg aattgtcctt agaacaatca
     9661 tccactaata aaagctgcca atttttataa gtttgatttt ggattgactt tatcgtctct
     9721 gataaaaact cttctgaatt ataaacagga gtaattatcg atactaaagg ctcattcata
     9781 tttttactct ccttatatag caactagcag tactatacca acaagtttac ttagatacta
     9841 attttctttg tccaatatat ttgtaatatc ctaatgggaa taatgctaaa tacaacaatt
     9901 tatcagatac ttttcttaat tcaccaaaat aatgactatc agacaacaat gagtatctgg
     9961 ctaaatttat gtaatttttt atccacatat atggatatct acttaatgga tatgtatttt
    10021 gatttattaa atataaaaac tctaatgctc tacctcttaa tgcattttta cttaatatat
    10081 tcttagtcaa accatcatcc aaataatctc ttattatcat aggtttattt acacatataa
    10141 ctttatagta tttagacatt tcattccata ctacactttc tggtaaaaat ctcacttctt
    10201 ttctttctgg aaaaggaaat tttataagct catcagttac aaatactatg cttttatctc
    10261 ccttaacgcc atacttaaaa tataggtctg caaagtgaca tattttctta tcttcaggca
    10321 tttctgtccc tatcaaatct ccacttggat atacacaaag tccaacaact ccactaaatc
    10381 cttctttatt ctctaaagaa tcccactcat cattagcaat ttcaagagaa tctttaatta
    10441 aaccatcatc actatctaca atgaaaaata gctctccatc tgcatttttt acacctagat
    10501 ttattgcagt atgttttcct ccattttctt tgtatatata tcttatattt aatttatttt
    10561 ctgatataaa tttttcaact aactctttag tactgtcgct acttccatca tcaacaatca
    10621 accattcaaa atcattgaaa tcataagttt gagcttttaa atcattgtac aaattttcaa
    10681 gtaaatgagc tctgttatac gtcggagtaa atacagtaaa tttatattta tacattaatt
    10741 aaatcttcct ctctgtctaa ctttttttga ttccgtttat tccataatta ataaagtagt
    10801 atatagcttt gtataaaggt attttttcta tatttctata tatattccaa gtccacatag
    10861 cagcttttat tttattacca cttagggaat tgctagattt tctatataat gctagaactt
    10921 catttattcc atgagcaata tgcccttttt tcaatatcga taaccataaa acataatctt
    10981 catgtcttac accactcatt cttatttcaa aatccaattt agacttatca atcacaacag
    11041 taagacatcc taagatattt ccttttaata atcttctata atctacatta ggtggaactt
    11101 ttattacttt gtttaatttt ttatcatttt catccatcag ttcatatgaa gtaaatgaaa
    11161 ttacatagtc attttctaac ataaaattta cttgtttttc taacttacta ctattccatt
    11221 ggtcatcact atctaaaaaa gctataaatt gtcctgttgc cttacttagt gctaaatttc
    11281 tagcattaga gacaccctta ttagtctcag tctttataca ttttattcta ctatcctgtt
    11341 gcatataaga tttgactata ttaggactat tatctgttga gcaatcatca ataattaaca
    11401 tttcccattc ttgataagtt tggtttaata ctgattttat ggttgcttca ataaattttt
    11461 cagaattata catgggagta attatagata ctaaattctt cttcatatta ccttcctcat
    11521 catttataaa atatttattt aattttactt aatacacaat catatatcct cttgcagttc
    11581 ttattatcat tatatctgaa aaacttattt ctttcatttt tttggttatc cataacctca
    11641 aaattatttt caataatatc aataagttta ttcacggctt catcattatt ataagccaaa
    11701 gacccaaaca tttcactttt taaatctata tatgaatcaa ctttttccat atattcatcc
    11761 aaatcgaatt ggtaaaacaa gataggtcta ttcatataca aaaagtctat ggctacactt
    11821 gaataatcag ttatatttgc agctgatttt ctgatttcat ttgctagagt taccccttta
    11881 tctaaaaaca caattcgttt tcctgttata ttttctttta tatcatctat aaactcatgc
    11941 ataagatgat gcatataaat ttttacattt atatctttct catctaatat tttatttaat
    12001 ttgtcatcat ttaaaaaatt cattatattt ttaaaataat ctgtatccgt aaattttcct
    12061 gaactaaatt ttatccaatc tctccatgta ggagtatata atatatctcg agtttgtgca
    12121 acttcatttg ttggtaaatt gtcatatctt gcatttccaa ttatataaac tgagtcatct
    12181 aacattcccc aatcatttac cataacattt ctttcaaatt cagaacttgc tgtaactata
    12241 ttatagcttt tatataggtt atctataata tttttgaact ttttgttcat aggttttctt
    12301 tttttaaaac ttatagtccc atgatttaaa aacactttta tcgttttttt atgaaaataa
    12361 ttaagaacag gtacagcaaa attgtatggt gctatatctg ctgatggtgc atgtgaaaat
    12421 actatagctt ttgaattgta ataatacaga tagttttcta cacttcctct tattaatttt
    12481 ttgcctggaa ttttatcaaa tactttactg tctttattta ctacccaata agtttctaca
    12541 tcattatgtt cttttagcat atactcataa aaaaactttg aattatcatt gtatatatcc
    12601 cctgaatgtc cacctataag ccagatatct ctaccattaa acttatgctt attaaatgga
    12661 tatataaaaa aagccaaaag catatttatt aaaaatttaa ccttgtccat aaacaccatc
    12721 ctcattataa cttgtactgt ctaaatccat taattcttcc ttttatagtg gcactatgat
    12781 aatctctatt ggaactcaat ataagacctc ctatatttct tacaagtttt gataccataa
    12841 cttttgcaaa cttataattt tttctataga ctatttcctt tttgcaaagc gctccaaagc
    12901 ctcttccata gttataatct ttttgatact tttctttaga tttagaatgt ttttttgcag
    12961 gatggtaaat tatatcattt ccaaagtatt ttcctttaaa acctaaactc aataagttta
    13021 aaacataatc tatctcttca ccagcaccaa actctccacc aacacccaat ttttcatcaa
    13081 atcttgtata gtctttacct tcaaaatcaa taaaaaaagt tatggatgtt atagtatcca
    13141 aaacgttgga acttgtgata tcacaagttc catcatacat tttcttaaat gcatcaactt
    13201 tatttgaatc catagttttg caactgtata ttttataatc tttattttta ttaaagaaat
    13261 ttataacttt ttctaatgta tcattttcgt agacacaatc atcatcaggg aaacctataa
    13321 tttgccctac agcattgtct atgcctacat ttctgttata actcaatcct tttttagagc
    13381 tttttatata ctttatatct aacttatcaa tgtatttatc aactatttct ttaactttgc
    13441 tattatcatt ttggtctact actataagtt caaagttttt gtaagtttga ttttctaagc
    13501 tgtccataag taagtatata tcatcatatc tatttaatgt tggcattatt aatgatatta
    13561 acattactac ctcctacttt tcatcttcca caaatctatt tcccttttat catttaatat
    13621 cacttctttt attagtaata ctactgcaaa actaaatgaa gcttctaata ataaattttc
    13681 tgcaaaagag tatagtaaaa atataaataa agctactaaa tatgcttttt tatctttttc
    13741 tataaagctt tctattcctt tatacatcaa atacataaat aatatacaag caactactcc
    13801 tagtacacta attatatata cataagaact atctaatgga ttagttgctt ttatagcagg
    13861 actatatgaa tttccaaaag gttttagaaa gttccccttt tcagccaagt atacattcca
    13921 gaacttaggt ctacttgcta ataatctatt taataaagtg cttttatcaa atactgtacc
    13981 aataattaca ctaaataaag taagtattac cggtaatgtc tttaggagca ttggtagtcc
    14041 ttttattttt tttatatcta tccatcttat tatttctaca aatattaatc ctgctaatat
    14101 agtgaaaaat ccagttctac tatatgtggt ttgataaaca aaaaatgcag aaccaaataa
    14161 aagtattcta tcccatttat tataatcttt atatcttaag aatatatatg ctgcatatat
    14221 agtaaccata tgataaaata cactatttgg attaccaagt ccaaaatcca ttcttgtaac
    14281 aagcatatct attttttcta tatatctata gtggacttcc tgattaaata ttaatgaagg
    14341 aaataaatta aataataata tagctaccat taaaaaacat gccgatatta ttaaataatt
    14401 tttaactata aatttatcat ctatttgctt agctgataca gctaataaaa cgattaataa
    14461 tatagctcct tccctattta ataaataaag taagacacaa actccaacta gtaccatttc
    14521 tgttatactt tttctcttat caaataaaaa aagtagcatt agtattccta ttgaagaata
    14581 aaataacgct gatttaatca tccctatatc catatttggg tcaataaatg atatcatata
    14641 ctttctaagt aatatgataa acaaaacaaa tatataaaga tattttctaa tcataatatt
    14701 tctatcatca tttaaagtct tcattttact ttctcccttt tagcttcgta aagtattact
    14761 tgcaaatatt ttacaacact tccatatctt atttacacct gttaagtgtt tgctttctaa
    14821 gaaataattt ctctttttag cccaactttt tccccaccaa tgtatcccaa aagtgttttc
    14881 tgttatacat tctggtgtaa agtcttcttt aaatccaaaa ggataaaaat attctttgtc
    14941 gtaaacacgt attccttctt cataattatt tggatcaatc ttcttataat tcttttccat
    15001 aagatgagtc attatctttg gtattgtaaa caatgaagaa gtaagtactt ctttttcata
    15061 atactccaat aagtctttta aaaaaggatg tcctttttct acacctatta tagcaccatt
    15121 tatatactct ctatcatctt catacccaca gactagccta ttattttcta aaagaggagt
    15181 tatatccttt aatatttgca tatctgtatc catataaact ccaccttgtt catagagtac
    15241 ttttattctt gtataatcag atatgaatgc ccaaagtttt cttttataac attcttccaa
    15301 aaacttattt ccttttattt ctttctctat atcaaagttt ttttcattcc actctactat
    15361 ctcatattct ggaagctttt ctttccaaga atttatacat atattttcta tatttccttt
    15421 tggaccacca aaccatacat aatgtatttt tttaggaatc aaatgaacac ctcctacata
    15481 tattcttgtc tatcttcttc ttttaactgc tctactattt ttttgacatc ttgagcttta
    15541 tcttttctac aaaccataac tgcatcattt gtagatacta ctatcaaatc actaatacct
    15601 attgttgata ttaatttgtc atctgaatat ataattgaat ttttagtatc tatagtaata
    15661 ttttcacctc ttttgatatt accttcatca tcagttggat atattgcacc tagagaatcc
    15721 caagaaccaa catcattcca tccaaaatca ccaggaacta ctataacatc attacttctt
    15781 tccataatac cataatctat agagattgat tgtatagttg ggtatatttc ttttattttt
    15841 tccatttctt cttttgttcc taagtattta cttatatctt ctaatttttc atatactttt
    15901 ggcaagtatc tcttaaaatc ttctagtatt ttagatacct tccatacgaa cattccacta
    15961 ttccatacat actttttaga gtttacatat tctttagcaa tttcatagtt tggtttttct
    16021 acaaactcca ctacctcata tgcaacatct tctatagtat tttctctatt aaaatttatg
    16081 tatccatatc ctgttgatgg aaatgtagga gttatcccaa tagttactag tttatcatta
    16141 ttttctgcta tatagatagc tttttccaaa atagatttaa attctttttc atcttttata
    16201 taatggtcag ctggatatac acacataaca ccatcaccat attttttcat tatgttaaat
    16261 gctgcaaagc ctatagctgc tgctgtattt ctggcacatg gctctggcaa tatattacta
    16321 tccaaacatt tatcttttac tatatccttt aaagcttcca attgcttctc attggttact
    16381 ataaataaat catctttttt tgctagagaa tctattctgt ttatagtttc gtttattaaa
    16441 gcatcttctc cacttaaatt tataagctgt ttaggtactt cttgtctact aagtggccag
    16501 aaacgagttc ctcctcctcc agccattatg acattgtata ctttcattgt ttatctcccc
    16561 ttagcttttt atatagaatc cactacattt aatatataag atattaaatt gttcaactta
    16621 aattctacat cttcttgatt gtctgaattt gctccaaagt aaaatttaat tttaggttct
    16681 gtacctgaag gtcttatagc tatccaagag ccatcttcaa gtaaaaattt aagtacatct
    16741 gacttaggta aatcatcaac accatcttta tagtctaaaa ttttatctac ttttacatca
    16801 gccacattat ctattttaac acttctaaag taaagcatca tctcttttat tttttttatt
    16861 ccatctatgc cttttaaagt tactgacttt aagtcctctt tgtagtatcc aaatttatta
    16921 tatgtatcta tcaaagcttc atacaagttc ataccttttg aagaatagta tgctgccatt
    16981 tcacatatca taagtgaaga tacaacacca tccttatctc tagcatgagt tccaattaag
    17041 taaccatagc tttcctcata tcccattata aaacttctat cattgctttc ttcaaacgct
    17101 tttatttttt ctcctataaa tttaaatcct gttaaagtat ttaaacaatc tacattataa
    17161 gcctttgcta tttttgcacc tagttctgat gtaactatag tttttattat tgtaggattg
    17221 tttttaggaa gtttattttc ttcaactaaa ctctctaata tatatctaac taaaagtgaa
    17281 ccgacttgat ttccatttaa gactacatat tcaccacttg aatcttttac aacgatacct
    17341 actctatcac aatcagggtc tgttcctatt atcaaatcag tgccattatt ctttgccatt
    17401 tcaatagcta tattaaacac tgacttttct tcaggattag gatactttac tgttgaaaaa
    17461 ttagaatctg gcaattcttg ctccttaact accattacat tttcaaaacc acattcattt
    17521 aagacttttc ttactggctt atttcctgta ccatgaatag gtgtatatat tatttttaat
    17581 tttttcccgt attcatctat tatattttgt cttaaaactt gtttcttaac agcttttata
    17641 aactcatcat ctacttcatt atctaatata gttattaaat ttttagataa agcttcttca
    17701 aaatctatac ttttaatagt actgtaatca tcaatcttat taacttctgc tattatatca
    17761 tttgccatat ctatacagat ttgaccacca tctgagtcat atactttata tccattgtat
    17821 tctttaggat tatgacttgc tgttattact attcccatag cacactttaa atatctaaca
    17881 gcaaaagaaa gttctggtgt aggctttaat tcttcaaaaa tataagcctt tacaccataa
    17941 gcacttaaag tctttgctac ttctatacaa aactccctag acatgtatct actatcatgt
    18001 gctataacta ttcccttttg cttaccttct tctccttgag ttttcatgat ataatttaaa
    18061 acgcccagtg tcgcacgtct aacagtataa atattgattc tattagttcc cgctcctatt
    18121 atacctctta aaccacctgt tccaaactct aaatttttat aaaatctatc ttgtatttct
    18181 ttttcatcat cttttatact taatagctca tttttcgttt gttcatcaaa ataaggagaa
    18241 tttaaccaca tctcataatt attcttataa tccatatttt catcctttct gactctaatt
    18301 taaaatttca aggtaattat ctatcttcta agtagttttt gttttccttt tcttaaaaat
    18361 tcaaaagcta aatccatctc ttctacctta aataaaacta tcaatacagt atatacagaa
    18421 gctccaccta atactccagc tccaactgat ataatttctt taattgttcc tgaacccata
    18481 aatgcatata gattattata tatcaataaa gtagctactg ccatcacacc agatgctacc
    18541 aaacttttta gaccagtctt tataatttta tcccctccaa aatatccatt tttcttttta
    18601 agagatataa ataataatat aactgtaatt atgtttgatg tactagttga tatagcaata
    18661 cctgcatgac ccaatggtct tatcaatatt aaattaagaa ctatatttat tattaaagct
    18721 atacttccat ttatcattgg tgtttttgta tctgaaagag agtaaaatgc cctatataag
    18781 acatctctta ctccacaagc caataaacct agtgaatata atctaagcgc tatagatgta
    18841 aggtctgttg ataacgcatc aaatttaccc ctttggaata atattctaac aagaggctct
    18901 gctaatataa tagctcctat agatatagga agcaccacca atataataca atttgaagat
    18961 tttacaatag tgcttataaa tccttcttta ttatctttac cagacatttt tgctagttta
    19021 ggatatataa ctgttattat agatgtaaca aataatgcca taacaaactc atttagccta
    19081 tttgcatagt taagagcaga caactttcca tcacccaaag tggttgccaa taatcggtca
    19141 acaaatatat ttaactgtcc aacagctact cctaccaaca taggcaatac taggttgact
    19201 aactctttta tagactcatc ctttaaattt atatatggct tatacttgta ggacttctta
    19261 aacgcaaatg gcagttgaaa caatagctga ctcgccatag ccaataatgc tcctgctggc
    19321 agtatccata catttttacc tgcacttagt gctattgctg ctattattat aatattatat
    19381 gggattccta taagcccagg aattacaaaa ctgtcattca cctgtaaaaa cgaactaaat
    19441 atcttacttc cactcaggaa taaaactcct gaaatcatta ttttagtaaa taatattgct
    19501 gcttggaact tagcagggtc gtttctaaat cctgctgcaa atactgtaac aaattgctct
    19561 gtaaataaaa gaccaataat agctattact atagccatta tatagcatat atttaaaaca
    19621 ttattagtaa attttaaggc accttcatct ccgagtcttt tctttgtctc aaagtacata
    19681 ggtatataag ttgtagcaac tgcagagcct atgactgcta tgattaaacc aggtatattt
    19741 aatgttaata caaatacgtc tgcatacata cctgtcccat agaaatttgc aagtacaagt
    19801 tctctaagga aacctaatac ttttgaaaac atagtggctg ccataatcca caatgctgct
    19861 ttagctgttt ttgacatctt ctttctcctt atctaattta aactctaaaa gtagaacttt
    19921 taaaactgct tataaacatt attctaatta agtaaattta acctatgaaa agcttcctta
    19981 taaatcaaaa actatttcga ctcatatcta agtgccctaa atgtaatcaa tagtaataat
    20041 agtattaata ctatacaaga taagaatgtt agattaaata tgctcttaaa tagactataa
    20101 tcacctactg tatacccaac ttcaactaca tcatttatat tgctatcttt caagaattta
    20161 tcttgtgcac gacttaagtt atcactacaa atcagtattg gcatattttc ttttgctgat
    20221 actactccca aagaaatata ttcgtttaca tcagatctac taccaaagtc taatctagca
    20281 gtatagattt tctttatatc tttttcatca taaaacttct ttattaaatc aacatttgta
    20341 cttgacttat ttatttgctt tattccttct atatttttta agttagaatc aatctctgca
    20401 ataaactctt ttgaatcacc taatgcatat atttttttaa gatttttgcc ttttaagaaa
    20461 tcaatttgtt cctcaaaccc ttcatcttta gcccatatta ttggcattcc acttttagcg
    20521 gcataagaat atacagatat ggcgttctct aatcctgttg ttgagctaag taaaaatgct
    20581 tcatcaaatc ctactgttct atacatttct ctagctatct ttaaagatgt atcatatctg
    20641 tctttccctc ttattctttc tactttcatt cccatttttt ctatattacg ttcagcatct
    20701 ttagatactg cctttaaacc acctactatc gtaaccttct ctggacctag ttcttttaga
    20761 taatttcttg ttactctacc aagattttta ctcttagtta caactattgg agcatttttt
    20821 gcatatgcta aaggtgttgc acttatacta tctacaattg actcttcatt tacaagaatt
    20881 acttctttag aatccttaaa tgctctttta tttaattctg tcgaaatctc tggtatactc
    20941 ccttctatca attctctttt gggatttgca tctgcaaata ctcctatcgt actagaaata
    21001 gagattaaca aaactaacga taatatagta gaaatttttt taatcataag ttctcattcc
    21061 ttttcttttt tgtatttttt gacaataaaa aagctctttt taaagagctt tttcattgat
    21121 tagtaatcta atattttaaa attatctagc tccgtctcct gtcaatacta ctttaacagt
    21181 tttaaagagt atcttaaagt caagtaatat actcctattt tttatatagt aaatatcttc
    21241 tttaagtttt tcttcaggag tgatttcgta tccaccattt acttgtgccc aacctgtaag
    21301 cccaggtttt atagcgagcc tgttgataaa tccaggtatt tcctcattaa attgaacagt
    21361 aaagttcggt ctctctggtc taggacctat taagcccatg tcccccttta atatattaaa
    21421 cagctgtgga atctcatcaa ttctagtttt cctaataaac tttccaacct tagttattct
    21481 aggatcatct ttttcagccc attgtgcgcc aaatttttct gcgtctgttc tcatagatct
    21541 caatttatat atatagaatc ttctcccgca ttttcctagt ctttcttgct tataagttat
    21601 tggtcctttg tcctctattt taataagaat tccaaatatt gctattaaag gtaaacctat
    21661 cacaagtcct attatagata aaactaaatc cataattctt tggtacaagt cgaataaaac
    21721 tgttcctttt actatgctat agtcaatctc acttgcaatt tctttatctg cccctggata
    21781 gcttttaatt ttaggtattt cacactcatc attagtatat ccagacatag ttacctccat
    21841 attggaataa aaattttttt aattcttaat ttcaacttat ttctatctta tttttgactt
    21901 atttctatat tatactcaat aatgagcaga ttatcaatac aagaaacatc attgtaaaca
    21961 atttgattct ttacaaaaat atatcatctc ttgttatatt ttacattata acaggagatg
    22021 atatgttttg ctttaatatt ttctatttta ttttactgta tcttctattc ctattccaat
    22081 ttgagttccc attgttttta ctttttctgc agtatttaac ttatccatat cgctcttatt
    22141 gtcaataaat gcacattcaa caagtattgc tggataattg ttatttctta acacatatag
    22201 gtaatctttt ccattatcaa gtgttcttgt cttgattcct ctatttttca tgttaaattt
    22261 ttctagtatt ctttttaaga tatttgatgc tgcagtctta gttgttccac catttttatc
    22321 ttttactttg taataaattt ccacaccatt accagctcca ttagacgcat tgtaatgtat
    22381 acttgtaaat aaatctggct ttatagtatt tgataatgct gttctttctc ctaaagccat
    22441 tgttttatct gtatctctag tcataacaac atttatacct tttgaacgta aatattcagt
    22501 agttgctaat gctgtattaa gagtatattt cttttcttgt gctccaccat ttaatccact
    22561 tgttgcccct gaatcagaac ccccatgacc tgggtctatc actacagttt ttcctgctgc
    22621 tgaacctgaa ttatctggtt ctgttggtgc attgtgtttt gataaactag atgctataga
    22681 atttataaca ctatctttca ttccatcacc aatttgatat actgtttctg ctgttttttc
    22741 tgataaattt gtattatggt atgcagaaac ataagttttt ggagttataa gtattggagc
    22801 ttttaattta gctgctaaag gtcctgctgt tattgaatca actattatgt cagattttgc
    22861 aactaacatt gcactcaact ctttatctgg atagaaagtc ttaattacat ttgcatttgt
    22921 ttcatgtcta tctgctccag aaactctatt ttttgaagta ccattttttg ctattttaga
    22981 tatttggtct ataatttttg atgataaact ttgactacca cctatgtaat aagcttcttc
    23041 taaaccttgt gaagataacc aattatatgt accttgtggt acagaatctt tattagctaa
    23101 tattataggt tgttggtctt caccagcttt tgatgaaata ttaagagcat catattcacc
    23161 agcatatcca tttgctatgt atattttatt aacatcatga tacttatcta tttcttttgc
    23221 tataagtaaa gatgtttcat gtctatcaac tccaccaata cgagtcacat ttacatttac
    23281 agcagattta atttgagaca ctgccttttg tgaaactgaa ccatcatcac ctataattat
    23341 tacatcactt ggatttagac gttttaactc tgcttttgta ctttctggta tatcatcttt
    23401 atttgcaagt aatattggtg cgtcatatgt agatgcaagt ggagtagctg taattccatc
    23461 tgctgctaat tctccattaa ctattacaac cttactagac ccatctttcc aaccttgttt
    23521 acttatctca acagcagttt caaatctagt agctccagca agttttttta ctggtgcaga
    23581 agtttttctc atatattcat ttactgcttt tgttgaattt gcatcactta ttataaactc
    23641 ttgtgtaaca tcataatcct taagatttaa tcccatagac tctaagtctt ctttgtttaa
    23701 aactttctgt tcatctttac catttatttt tatgctttga agttcccata catttaagta
    23761 ttctgggtct ttgttatagt ttattacacc ttcatatgaa tctgtctttt cattgtaatt
    23821 taaagtagct tcaagtggca tatcataaca cataaaattt aatgttattg aatcagcatt
    23881 taatttttct ttaaatttta cacttgcatt agatttactt cctaacacta cactttttct
    23941 ttgtaaattt atattaatat ctgaattaga ttctgtattt gcagtagtat tttcagccgc
    24001 taaagctaca tttggtgcca ccattgctac agacaacatt cctgttgcca ataattttgt
    24061 tgtttttttc atcatatccc ccctaatttt ttggttcttt aaatctttaa atctttttta
    24121 atacatctta agcttgtttt atattacata aattcaatat tatgtaaatc ttttttcatt
    24181 atagcacaaa aaattacaaa ttgtaaactt tttgtaatct tttatggcat ggtttttaaa
    24241 ttttatcgag gagaaatttt gattatattt gatagtttct gtatatttga acaaatcaaa
    24301 gactttatca tctaaaaata ttgcactaaa ttttaataaa tatttttttc ttgtacatta
    24361 tagacattac tataacccag aataggacat acaataaaga aaatataaga gaatcccaag
    24421 ttgtacctgc ccaaggtgtt ataagttcat atgtagtcca ctctaccaag gtagttggat
    24481 aatcgacttt gttcgtaagt tttggaacat tccaaaaaac atgactcaat atctcaaggc
    24541 acatatatgt aaaaatgggg ctacttccaa gtgctattat tggggtaaat attttacttt
    24601 tatttttgat gtcgcatata aaataaaata ttgacagtaa tataccataa ctgccagcca
    24661 ttaaaagtac aaatgagcta gaccataatc ttttattaaa tggaaaatac tgattaaaga
    24721 taaaagcacc tattagcagg attatgctca taaccagtat tttaaagaat tttttatatt
    24781 ctcctaggtt ttcttttaaa agtacacatc ccatagtgca tcccaacata cctgaagaaa
    24841 ttgctactat actagttaaa aaaccatctg gttcaaattc cggcttgtac aaatgtcctt
    24901 tgaagaaatg taaatccact aactgtgcaa gagaaccttc tagctcaaat ccataaggtt
    24961 ttgctaagat gtagtaccca actattataa atgtagctat acatagaaaa gtaattattg
    25021 tagcagtact tccaacattt aattttttta gcagtaaata tgctaaacta gtcacaaaat
    25081 agactaatcc catacgttga agtactccaa gtattcttac agtatctaaa tcaggatttc
    25141 ctaggtaatt taagaaaaat ccaaataata tcaataagat acttctcttg aatatactaa
    25201 gtattatact cagtgtggat ttattatttt ttagtttact atttattgat attggaattg
    25261 taacacctaa agatataaca aaaaaaggaa atgcaaaatc tgcaagtgtt acaccatgcc
    25321 atacagcatg tcttaattga ggatacattc tcatccaagt cccaggatta ttgcagacta
    25381 tcatgagggc tattgatagc cctcttatta tatctattga tttaatccta ctattcataa
    25441 gtttagaatc tacagcatta tttgaaatt
//
LOCUS       DBGJFU010000046        25436 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.046, whole genome shotgun sequence.
ACCESSION   DBGJFU010000046 DBGJFU010000000
VERSION     DBGJFU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.046"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            464..2320
                     /locus_tag="ACOIAS_001853"
     CDS             464..2320
                     /locus_tag="ACOIAS_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2701565.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    2403..2643
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2709..2944
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3029..4246
                     /locus_tag="ACOIAS_001854"
     CDS             3029..4246
                     /locus_tag="ACOIAS_001854"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGZ2701566.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            4363..4857
                     /gene="cdeM"
                     /locus_tag="ACOIAS_001855"
     CDS             4363..4857
                     /gene="cdeM"
                     /locus_tag="ACOIAS_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HGZ2701567.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGENRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            5122..6387
                     /gene="hisD"
                     /locus_tag="ACOIAS_001856"
     CDS             5122..6387
                     /gene="hisD"
                     /locus_tag="ACOIAS_001856"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGZ2701568.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6590..7342)
                     /locus_tag="ACOIAS_001857"
     CDS             complement(6590..7342)
                     /locus_tag="ACOIAS_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2701569.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7460..7729)
                     /locus_tag="ACOIAS_001858"
     CDS             complement(7460..7729)
                     /locus_tag="ACOIAS_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HGZ2701570.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            8204..9298
                     /locus_tag="ACOIAS_001859"
     CDS             8204..9298
                     /locus_tag="ACOIAS_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2701571.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            9300..10250
                     /locus_tag="ACOIAS_001860"
     CDS             9300..10250
                     /locus_tag="ACOIAS_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGZ2701572.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKVDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            10243..11427
                     /locus_tag="ACOIAS_001861"
     CDS             10243..11427
                     /locus_tag="ACOIAS_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701573.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11440..12936
                     /locus_tag="ACOIAS_001862"
     CDS             11440..12936
                     /locus_tag="ACOIAS_001862"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701574.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEECMKICEDFDFNIDPHMVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVND
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12959..13909
                     /locus_tag="ACOIAS_001863"
     CDS             12959..13909
                     /locus_tag="ACOIAS_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701575.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13930..14892
                     /locus_tag="ACOIAS_001864"
     CDS             13930..14892
                     /locus_tag="ACOIAS_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HGZ2701576.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            15264..15896
                     /locus_tag="ACOIAS_001865"
     CDS             15264..15896
                     /locus_tag="ACOIAS_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2701577.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            16322..16399
                     /gene="kdpF"
                     /locus_tag="ACOIAS_001866"
     CDS             16322..16399
                     /gene="kdpF"
                     /locus_tag="ACOIAS_001866"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGZ2701578.1"
                     /translation="MWFLAVVIIMLIIYLVYALINPEKF"
     gene            16446..18122
                     /gene="kdpA"
                     /locus_tag="ACOIAS_001867"
     CDS             16446..18122
                     /gene="kdpA"
                     /locus_tag="ACOIAS_001867"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGZ2701579.1"
                     /translation="MVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDNFI
                     YKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGLSF
                     NTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKKKT
                     LGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGPVA
                     ALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKNKK
                     QGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSSLF
                     TTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVFLC
                     GLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGFHG
                     LSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLASK
                     KAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            18147..20204
                     /gene="kdpB"
                     /locus_tag="ACOIAS_001868"
     CDS             18147..20204
                     /gene="kdpB"
                     /locus_tag="ACOIAS_001868"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGZ2701580.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            20223..20849
                     /locus_tag="ACOIAS_001869"
     CDS             20223..20849
                     /locus_tag="ACOIAS_001869"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGZ2701581.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVEDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            21086..21994
                     /gene="cysK"
                     /locus_tag="ACOIAS_001870"
     CDS             21086..21994
                     /gene="cysK"
                     /locus_tag="ACOIAS_001870"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGZ2701582.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            22021..22611
                     /gene="epsC"
                     /locus_tag="ACOIAS_001871"
     CDS             22021..22611
                     /gene="epsC"
                     /locus_tag="ACOIAS_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HGZ2701583.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22771..22959
                     /locus_tag="ACOIAS_001872"
     CDS             22771..22959
                     /locus_tag="ACOIAS_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGZ2701584.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(23013..23882)
                     /locus_tag="ACOIAS_001873"
     CDS             complement(23013..23882)
                     /locus_tag="ACOIAS_001873"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HGZ2701585.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDVINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(24079..25323)
                     /locus_tag="ACOIAS_001874"
     CDS             complement(24079..25323)
                     /locus_tag="ACOIAS_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701586.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYNMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9790 a         2713 c         4573 g         8360 t
ORIGIN      
        1 aagaaatatt gacatttaag ttaaaaagtc tagaattttt ctgggctttt tttattttta
       61 gtataacttg acaaatttta tttaatggga tagttttaat ttataatcat ctttaaaaag
      121 atgactattg ataagtaaga gataaaattg gtaagtatca aaaaatatac taaataaaaa
      181 ttatagtcct aatgagttaa tttctgtatt ttcaaataat gcagtattta gtcatgtgtt
      241 tttatatgcg cctgtatttc taattagcaa tatattagaa taagctactg taattagatt
      301 tagaaaaaag cgacataaat aattataaac aaataataaa ttacataaaa ttaaatttat
      361 tcacaagtat atcttataaa atatgttata attaatagaa aattgtcata taattaatta
      421 agtagtatta ttataaataa cattgaaagg tagtggttag aatatggata cccataataa
      481 atatgtaaat tttataaaaa atatacctgt acctttttta tattgtagaa ttgtaaagag
      541 acaagaagat atagaatatc gagtagaata tataagtaag ggtatgggca aagtattaca
      601 attagaagaa ggtatttgtg ataaaaatat actagatgta ttgcctgtat ttaagtctaa
      661 aaaatatttc aaagagttgt tctcaaatga agtggattgt ataaaaaggt acattccgac
      721 acttaaaaac tggataaata taaaaaaaca gataataggg gattcataca taattttgta
      781 ttttggtaaa attgtatttg attatagaca gataatcgat tcctttgata aaaaggaaaa
      841 agtagcttat attaaagacg aagaaggcat atatatagat tgtagtgaaa atttaatacc
      901 aatattaaat aacaacatta aaacaacgaa agatatcttt ggtaaaaatg atatagaagt
      961 atggggagag aatacaggaa aattatttag agatgattat agagaaggag tatctagtaa
     1021 aaaaagattt ttacaaaatc tttttgaata tgaagaaaca ttttttatgg ttgaaaaata
     1081 ttttttgtat gatgaagacg aacttttagg tactataggg atagtagaca atataatata
     1141 tagtggttac agtaatagaa attataactc aaaagattta atgaagatga tagaacactc
     1201 aattccagag aatatgtttt ataaagatgt gtatggaaat tatattggat ttaattcagg
     1261 ttttttgaat ttagcttgta tgaataaaga agaattatta gggaagaata gttataaaat
     1321 atcagaagaa gaagctttaa tagataagat atttgaaagt gataagggtg tagttgaaaa
     1381 taaaaaagta gttacatttg agttaaatat aagtatgaat gatgaaaaca agtgtattga
     1441 gattacaaaa aggccgtttt ttgatagcta tggaagtgtt attggaataa ttggaacagc
     1501 aagggatata agtagaagaa aaagattaga agaggaaatg gataaaacta ggatggaatt
     1561 ttttgcaaat ttatcccatg aacttagaac gcctataaat ttaatatcat cttcattaca
     1621 agtgatagaa aaaaaagaag cagatttaat agaatcaaat gatactttaa aaagaaatct
     1681 aggtataata aaacaaaatg gaaatagaat attgaggtta gtaaataatg ttatagactt
     1741 tacaaaaatg caatcaggat atttagattt taaaccagaa gaatctgata taattgcatt
     1801 tatagaggag atatgtatgt ctgtagctga ttttgcaagc caaaataata tacaacttac
     1861 atttgataca gaaatagaag aattttcaat gttatttgat tcagaaaaat tggaaagaat
     1921 aatattaaat ttactttcta atggaattaa gtacaataag aaagatggaa aaataaatat
     1981 atttttatat gtgaaagata atgtatttaa tatgaaaata tctgatagtg gtataggtat
     2041 accaaaagaa aaaatagata aaatatttaa taggtttgaa cagattgaca atgaactttc
     2101 ttacagagtt aaaggaagtg gtattggact ttcattagtg aaatctttgg ttgagttgca
     2161 tgagggaagt atatctttaa aaagtcaact tggtattgga agtgaattta tagtttcatt
     2221 gcctgttaga agtaaaaata atattgaaaa atataatcat aaaagagaaa tcagcaatga
     2281 actaagtaaa aaattagaaa tagagttctc ggatttataa gtttaaatat aaaaaataaa
     2341 attttaaaaa agtattgaca attgatttag aagggtttaa aataaattac aacataataa
     2401 ataaaaaggc gatgaatagg aagagtaaat ctaattcgat taccagagag aagagtccgt
     2461 tggctggaag actttttaaa ttagagggat tgaaaactac ctaggagcta ctttctgaag
     2521 tcatctgcat tagtagattt tagtaagaat agtcgttagc tgcgttaaag tgtaaagtga
     2581 gttttcatga catatgaaaa ctaaattagg tggaaccgcg ggaataatac tctcgtcctt
     2641 atgttataag gatgagagtt ttttgttatt taaaaatata aacagtaatc ataataatta
     2701 tagaaaatat aaaagcaatg aataggaaga gtaaatttaa ttcgactatc agagaggaaa
     2761 gttcacaggc tgaaagactt tctaagtttg agaaattgaa aactacctag gagctgtggt
     2821 ctgaagttac tttaaaaagt tttagtaaga ctaaccgtta actgcgttaa agtgtaaagt
     2881 ggattttatc aataaatgat aaaatcaaat tgggtggaac cgcgagtata gactcgtccc
     2941 tttatatagg gatgagtcta tttttattgc aataaaagat tgaaaataaa aaaaaatcgg
     3001 aaaattataa atttaggagg aaaataaaat ggagaataaa gtgaaaattg gagtactagg
     3061 atatggagtt gtaggaagtg gacttataga tataatagat aataataaag aaaaaagaaa
     3121 tatagaaata gttggaattt tagttaataa tttggaaaaa cataaggata aaaaatattc
     3181 taatataata actaataata tagatgatat atttaataaa gatatagata tacttgttga
     3241 agtcatgggt ggtttagaac catcattatc ttatataaaa aaagcattaa acaataaaat
     3301 tcatgttgtt acagcaaata aagatttgtt agctgaatgt ggagatgagc ttgcaaaatt
     3361 agcaagtgaa aataaagtaa gtattaaatt tgaagcttca gttgcaggag gtataccagt
     3421 attaaaacca ataatagaat cactagaagg aaataatata gatagtataa atgcaatttt
     3481 aaatggaact actaatttta tattatctaa aatgtatgat gagaatctat cttatgatat
     3541 ggcattaaga caagctcaag aattaggttt tgctgaagca aatccagagt cagatgtatt
     3601 agggtatgat gctgctagaa aactatcaat attatcaact ctagcttatg acaatagagt
     3661 atattggaag gatgtgtatt tagaaggaat tacagatata gatgaaaaag atattgaata
     3721 tgcaaaaaaa ttaaattgta aaataaaatt gattggtcaa agtaagtatg aaaatgataa
     3781 agtaagtgct tttgttagac ctgttttagt agaaaaagat aatatccttg ctagaataga
     3841 caatgagttc aatgctgtca tagtgaatgg agattcagta ggagaagttt cttttgtagg
     3901 taaaggcgct ggaagtttag caacaggaag tgcggtttat tcagatgtta tagatataat
     3961 agataataga gtttcaagta tagattcttt tactaaagat aaaatacaag ttaataaaat
     4021 agttagagaa aagtgtggag cacttcttag atttaaaaaa tgtaataaag atgaaattct
     4081 aaatatagtt gagaattgtt tagtaaaatt cgatatttta aatgatgatg atgaacttgc
     4141 tattatggtt tatgctgatt ctgaatatga aataaacaac agtttatgtc taataaaaga
     4201 taaaggatac tgcgaaaaaa tgaataaaat gttgaaaata agttaaaaaa gaaaaaatat
     4261 aagtttatat gtaacatatt ttgacgattt taatactata tagtatgaga tgaaacaaaa
     4321 catctcataa aaaaattaaa attcaaggga gagatatgtt atatggaaaa taaaaaatat
     4381 gcaaatggtg gttattcaga agactggtat gaaagaggag aatctacagc taaatggttc
     4441 cagaatgata gagaagaata tgaaagagca gcatacgatg aagatagaga aagaagaggc
     4501 tcaaactgtg gatgttcaga ttcaggagaa aatagaccta gaaactgtga aagatttaga
     4561 agagaagcag agataagaga aagagaagca agagaagcat tctgtgaatc ttcagagaga
     4621 aagaaagaag cactagcata tgaatgtgaa gctagaaaat tatgggaaga agcagaaaaa
     4681 tactgggatg aatattcaaa atacaactat aaaggaatcg aatatttagc agaagctgct
     4741 agattatttg atgaaggtat ggaatgtgaa gctagaagaa atggaaataa tggaggaaac
     4801 aataataatt gttgccataa atgccataaa tgtaattgta actgctgtag aaaataaaaa
     4861 aattaattga caaatcaata gttaaaatat atcatattat gtataataaa gagaaaattc
     4921 agaaaaaaat aatgattaga agaaatataa gtatatttat tagacaaata gtgctcagtt
     4981 agtgaggtga gtacaatatg attaaactcc catcctctag cttttgatag agggtgggag
     5041 tttttattta tttatatatc ttaaatgagg ataatattgt aaaataaatg agtatgagat
     5101 tgtaaaatta ggaggtaaaa aatgaagagt attaaaacat caagtatgaa agaaaaggta
     5161 gatgaaaaga tattaagaga gcaagtaagt tctataatag aagatataag aaataataag
     5221 gatgttgcac ttaaaaaata taatgagaag tttgatagaa atacaagaga tgaatttaga
     5281 attactaagg aagaaataaa ggaagcttat aagcatgtag atgacgaatt tataaataac
     5341 ttaaagattg ctgccaaaaa tataagagaa tttgctaaag ctcaaaaact aagttttgaa
     5401 aacccttttg aaaaagaaat ttatccaggt gtaatcttag gtcaaactaa tattccaata
     5461 gagtcatgtc ttgcatatgt tccaggaggt cagtatccat tgttttcaac agctcttatg
     5521 cttataatac ctgcaaaagt agcaggagta aaaagaatag tggcatgttc accaactatg
     5581 aaaaatactg aaaagataaa tcctaagaca ttggtagcta tggatatagc aggtgctgat
     5641 gaaatatatg caactggagg agttcaagct atagcagcat ttacatatgg aacagaaaag
     5701 attaatccag ttgacataat tgtaggacca ggaaataagt ttgtaactga agctaaaaga
     5761 caatgttatg gacaagtagg gattgatttt gtcgctgggc caagtgaagt tcttataata
     5821 gcggatgaaa cttcaaatcc agtttatatt gcagctgatt tattagcaca atgtgagcat
     5881 gatttaaatg caagaggaat attattgaca aatagcttgg aagtagcaca aaaagttgaa
     5941 aaaaatatag aaagtatgct aaaagatttg ccaacaaagg acatagcata tagttcatgg
     6001 gaaaataatg gagagataat attggttgat gatatggaag aagccattaa aataagtaat
     6061 ttttatgcac cagaacattt agaagttgca gtaaatgagt ataatgatat ttgtgataga
     6121 ttaactaact atggttcttt atttattgga aatctttctg ctgaggtgtt tggtgactat
     6181 gtttcaggta ccaatcatac attaccaaca cttaaagcat caagatatac tggtggtgtt
     6241 tgggttggaa cttttatcaa aacttgtaca aagcagatat ttaatgaaaa agctatacaa
     6301 tcattagcac ctgtagctga aaagctggca aaagaagagg gattatatgc acatgcaaag
     6361 gctgctgagg ttaggtttaa aaagtagtaa aaaattttat tttggcagtc gagatttagt
     6421 gttagactga gataaggaaa acgctgagaa cccgaataaa tactatattt tgtgggcctc
     6481 ggcgtttctt ttatatctgt atataatagg ataatactac ctgtgtgttt aaatgctagc
     6541 ttaatatttc ttttctaaat aatgtttatg aaatagttaa tatttttagt taatacttga
     6601 aaaaaacttt tgctaactgt gcaaattcat ctgcattcgt caatatagca gggtgtcctc
     6661 cctgactaaa aatgtgaata cttgcttttg aaataaaagt tgccatttgt ttgtactctt
     6721 cttcaagatt atgacgacac atttcgtctt ccttacttcc tgtaaataaa attggcattt
     6781 ctaatttaca caattcttta tgaaacaaag ggcgtttctc gtttgcacat tgcaatagag
     6841 cttttgtatc aagattaaca acattctccc aatcttttcc atgacaccat tcatagaatt
     6901 ttcgtgcttg aatatcttgc tttgattttt cccttccagt gattagatta tctatgaagt
     6961 tatcattaag agtacgtcca tcaaagctat ctgctataat tgcttcaact aaatcgggac
     7021 gttctaatcc agtattaaca gctgcccatg ctcctccact tgttccaatt aagccaactt
     7081 taccacaatt caaatgttca atcaatgcta ttgtctgtaa ggcttcatca tgccatatgt
     7141 caggagaaaa ttgagaaact ctatccgact gtccattacc taaaaaatca attaagatac
     7201 atctaaaatt ttctacatat aatggcacaa gtgctttaaa catattagaa gaaccagtat
     7261 taccatgcag gaaaattatc ggtttacctt gtccatattc tttataaaaa caagaattac
     7321 cttgatactg aaaatatgac atatattttc ctccttttta tttgttaagt taattataac
     7381 aatataggta tgaatgaaaa acctcatgtt ctttcttaga ataaaaatgt gccacgcata
     7441 aaacgtgaca catttctaat tataaatttt gctgtttaag tattctttgt atgcttttct
     7501 ctgtcaaaaa gtatttctct gataactgct tgatactcat tccattttgg aaatttatac
     7561 agatttgaga attacgagaa gctaagactt gtttggtgtt tgtgttttca ccccaatgct
     7621 ttttgtgctc ttgctttttg ggaatataaa tcactttacc atctatatat tcttgcactt
     7681 gctcaatcag ttctttaggc agtaagtcaa tcgaattttg atagctcatt ttgcacctcc
     7741 taaataatta ttctttagga ttagcaaagg ctactgccac tcacaattaa aaataaaatt
     7801 ttttattatg tacaatagcc tttgctatgc acaagttcca attcccataa actaaatact
     7861 cctttctggt ttgaaaaatt catctgaaca ttgatttcgc ttttaagata gagttcaatg
     7921 acaaatttac atttttatta tatatattca gatagataat tgcaactgtc tttgtatgag
     7981 gatattttat tatagtcaat actataatat gtatttatat catcaaacta atatgaacag
     8041 ttgaaaggaa tatgaacaat aaaacttagt caggaagaat aattatgaag cagacaatag
     8101 agaaatttga ttttaaagtt ttattgaaaa tttattgaca aatttaactt gtaaatatta
     8161 tactaaactt aataaataaa gtttataaag taaggtgttg tctatgaaaa atatagttgg
     8221 aaaaccgcaa acgataaaaa aagtaaatga agatttaata aaaaatatta taaaaaatga
     8281 aggtcctata acaaaaccag aaatttcaaa cattactgaa ttgagtttag ctacagttaa
     8341 taagattgta gagcaattag ctttgaaaaa tgaagttaaa gtaagtggac taagtgaatc
     8401 tactggagga agaagagctc aattatttga aataaatgca aacttagaac acataatagc
     8461 tttatattat tatagaaatt gttgtatagg ggtagttgcc aatttattag gtgaaataat
     8521 ttatcaagaa gaatttaata taagaatgga tacatatgaa aacgttaacc aagatacttt
     8581 ttttgttata gatactctaa taaaaaagga aaaaggaaac aatataaggg ctattggaat
     8641 tggtgtccca ggagttgtaa aaaaaggtgt agttaaaaat ataccaacta taaaaagttg
     8701 ggaaggtata aatttagaaa atttaataaa gagtaaatac aatactaaag tgtttataga
     8761 aaatgataca aatattacta ctattgggat ttatcaaaaa cagtataaag acaaatataa
     8821 aaatatggct cttatgtatt tagaagatgg tattggttca ggtatagtta taaatggtga
     8881 attgtattta ggttctacaa attttgctgg agagttaagt tatctaaata tagaatttgg
     8941 taagtataac tctaacaact taaatcttga agattatatt atgagtttaa gaaaaagata
     9001 tttagaaagt aaagataata aatttaagaa agaaatttta tctataatat caaactttat
     9061 aaggactctt gtatgtgtcc taaatccaca agctatgatt atacagtgta gtatattaac
     9121 caagagtgac atggagttta tagaaaaaga tgtaagcaat aatgttggag aagaaaattt
     9181 tcctaaatta ataaaagtag agtcattgag agaaggaagt ataaatggag taattagtat
     9241 gtgtttaaaa gaaactgact atcaatattc cttatctaat aaaaaaggag gatattaatg
     9301 tgaaatattt ggtttcaata gatggagggg gaacaaaaac taaattttgt gtaagtgact
     9361 tagatggaaa catacttaaa gaacatacca ctggttcaac taattataag tcagttggaa
     9421 ttaaaaaaac atatgaaaat attaataatg gatttaaaaa aatattaaaa gatttatata
     9481 tcgattatga cgatattgag tacactgtat ttggtatatc tggttgtgat tctcctaatg
     9541 attataagat tataatggat gaaatattaa aaattgggat taacaaagaa aaaatatatc
     9601 tggcaaatga tgcagtttta gctttttatg cacaagcgga ttcccctggt ttagtaattg
     9661 tggctgggac aggttcaatt atactgggaa ttaaagaaga tggagaaata tatagagttg
     9721 gtggatgggg gtataatttt agtgatttgg gttctggata tgatataggg agaaaattat
     9781 taaagaaagt attgctttat tgtgatgaat gtcatgagta tagtgattta tttagttgtg
     9841 tcttggattt ctttggagca aatagttttg aacaacttgc atatatgatt actgatataa
     9901 acaataatgt tgaaatagca aacttagctt cattagtgat tgattgtgcc gaacaaggtg
     9961 ataaattggc tattgaaata ttaagagaat catcaactga actatctaaa ttagctcaag
    10021 taatattgag aaaaatatct aatccaatga aagtggataa aagcgaaata aatatagttt
    10081 tgtctggtgg tacattaaat aaaactgtat atgctcaaat gttaatgaat aatttaaaaa
    10141 cagaaaatac agataaaaat ttaaaattta tatctcaaga aaatcagcca gtgtatgggg
    10201 ggataagact tgctatggca ttagctaata ggagggtcaa gcatggatag aaaaaaggta
    10261 atagtacaaa tttttactgg aggttttaag gctaaagaaa taaagtttaa tgatataaaa
    10321 tgtaaactac tagaattatt ggactgtatt gatatagaaa aggttatcat gggatggagt
    10381 gtagatgaag aattatatgt agaaactaaa aaactattaa ataaatataa tgttgaacta
    10441 tatctttggt tgccatgttt ttctgaaata ggattacttg aaaaaagtaa tttgctaatt
    10501 gattatatgg ggaaggaagt caagggctat gctttacaag aggatgaaaa ttttgagttt
    10561 tattgcccaa ataataagga aaatataaaa aatataaaga aaatatatag tacttatttt
    10621 agtgatatta aatttgatgg aattttttta gataaaataa ggtatggttc tttttcgaat
    10681 aaactaaatg gtgtttttaa ttgtttttgt aaagattgtt taaatatata tgaaaaaaat
    10741 aatattgata aagagtcgtt aatgagagaa atgaataatg tgtctaaggg agttatagat
    10801 aatgaagata ctccatttgg tataacagaa tacattaatg gaaaatacaa atttaaaaat
    10861 aaaatatggg aagatttttt tgaaacaaaa aatcaaaata tatacaaggc attattagag
    10921 ataagtaatt attttaaaag taagggattg aaaataggta tagatacttt ttctcctttt
    10981 atttcttact ttacaggtca agatttagaa aaattacaag aagtagcaga ttttataaaa
    11041 ccaatgatgt atagaactac aaaagctcca gctggtttac catttgaatt tgaaaggttt
    11101 attgatgaat ccatacataa gaataaagag attggaaaaa gaaatttctt agatatttta
    11161 ggaattaagg agtatgaagg agatgtatat cctatagaat ttgtactaga ggaaatcgat
    11221 tttatgacaa gaaatcttaa taaagatata tatgtaggag ttgaaataaa taaaaaggaa
    11281 attatatctc ctgttacacc taagtacata agtgaaaata tttcaaatat aaataagaca
    11341 acagtaaaag gatatgtttt atcttgggat ttgttgtcag caccaaaaga aaatattgat
    11401 gaaattataa aatggtttcg gaattagtga ggtgataaag tgagtaagat attggaaatg
    11461 caacaaatat ataaaagttt tggaaaggta gatgttttaa aagggataaa ttttgagtta
    11521 gaaaagggag aagtaagagc tcttttggga gctaatggag caggaaaatc tacacttatt
    11581 aaaattttag gaggagttca ttctcaaact aaaggtaaag taattttgac tggtaatgaa
    11641 ataatgttta aaaatccaca tcattctaaa gaaaatggta ttagtataat ttatcaagaa
    11701 ttaagcttga taccaacgct tacagtactt gaaaatattt ttcttggtag agaaatttta
    11761 aaaggaagtg tgtttttaaa caagaaagac atgcttgagg aatgtatgaa aatttgtgag
    11821 gattttgatt ttaatataga cccacatatg gtagtttcta atttaagtgt agcaaaccaa
    11881 caaatggttg agataatgaa agctgtttca tgtaattctg aaattatcat aatggatgaa
    11941 cctacaacat cacttactaa taatgaaaaa gatgggctat ttaaaataat aagtagacta
    12001 aagtctaaag gtaaaacaat agtctatatt tctcacatat tagaggaagt ttttaaagta
    12061 tgtgatagtg caagtataat gaaaaatgga attatgattg gaacttatga tgtagaggat
    12121 ttaactaaat taaagataac tcaacttatg acaggaaatg ataatcacaa acttattgag
    12181 aagaaggatt atacatttgc aaaatatgaa gagccaccag tactagaagt aaagaatata
    12241 agctggtcta acaaaataaa aaatgtttca tttaaagttt atagaggcga agttgtaggt
    12301 cttgcaggtc ttgtaggttc aagaagaact gaaattataa atttgatatt tggtatagat
    12361 aataaagata gtggagacat ttttatcaat ggtaaaaaag tagacataac ttctcctaag
    12421 gttgctataa aaaacaaaat aggattgatt cctgaagata gaaaaaatct aggattaatt
    12481 ttagaacaag ctatatacac aaactcatca tcagtacaaa ttgataaatt caagaaattc
    12541 ttgtttatag atagaaataa agaaattgag ttttctaaaa aaggtgttaa ggaattagga
    12601 ataaaagtta atgatgtaaa gcaagaagta aaagagctta gtggtggaaa tcaacaaaaa
    12661 gtagtagttt ctaagtggtt agagaaagat ttagatttaa taatatatga tgagccgaca
    12721 aagggaatag atattgctgc aaaagaagat attttcaata caatcaaaga attttcaagt
    12781 aaaggaatag gagtaatatt tatatcttct gatttagaag aagtaataag agtaagtgat
    12841 agaattttag taactagaga aggttctatt gtagatgatt taaaaaatga gaatatacag
    12901 gttcaagata taatgaacag tatatttaat gtataaaaaa tgaaaatgga ggactgaaat
    12961 gaagaaagct tttgagaagt taaatttaag agattatgga gtattattag gttttttagc
    13021 tttatgtata gctataagta ttgcaactcc atcattttta ggtaaacaaa atatacttaa
    13081 tcttttacga caatcttcta taattggaat aatttcagca ggtatgactt ttgttataat
    13141 atctggaaac tttgatatat cagttggagc agtagcagct cttgcaggtg ctataacaat
    13201 gaaatttgcg actggtggaa ctaatttgtt tgcatccatg ctcttaggtg tagcgatttg
    13261 tgcagtaata ggattaatca atggtatagt agttgcaaag ataaatgtac catcattaat
    13321 agccactatg gctatggtca ctattgtaag aggattgctg ttgatgctta caggaggata
    13381 tccaattact gaaaatatac caatacttga ttacattgga aatggatatt tgtttaatat
    13441 accaatacca gtagtaattt tctttatagt ggtgttagct tcatttattg tacttaataa
    13501 aactaagttt ggtagatatg tttattctgt aggagggaat caagaagcaa gtaaacttaa
    13561 tggtataaat gtagattctc acaaagtgaa agtatttatt attaatgctg tacttgcagg
    13621 aatagctgga atcgtactaa caggaagact tgggactgca actgcgattg caggtgaagg
    13681 atatgatatg gatgcaattg catctgtagt aataggaggt acatcagtgg caggaggttc
    13741 tggttctgta cttaaaactg ttataggagt attacttatg agtgttataa acaacagttt
    13801 taatcttctt ggaatagatg tatatttcca gtacatattt aaaggattaa taattttagc
    13861 agcagttgga tttgattctt atagcaagaa aaaacttgct tcaagataaa aaataagggg
    13921 gattttttta tgaaaaaatt aatgaaacat ttagcattgt tgctatcact tgttatgata
    13981 tttggtttag ttggttgttc aaatggtggt gacaaagaaa aaaatggaga aaagaaaata
    14041 gcagtattac taccaggttc aacaggttac tttgtagcaa ctaaacaagg tatagatgca
    14101 aaatctaaag aattaggtgt aagcgtggaa tatgcagatg ctcaatggga tgctagtaaa
    14161 caattatcac aagctgaaga ttttatggct aaaggggtag acatgattgc attatgtgga
    14221 gttgattctg ctgtaagtga gagaatagtt aaagctgcta atgattcaga cgttcctata
    14281 gtagcattta caaattctat aggaagtaac ccaactggag aatttaaagg tttagttaca
    14341 tatataggac aaaatgagga agaaacagga gcattaactg gaaaaattgc taaaaactta
    14401 ttaggagaaa ctggtgggaa agctgtatta atagaaggag taccaggaac tactccacaa
    14461 gtattaagaa aaaaaggtct tgaaaaagaa ttaaaagata gtaatataga aatagtttat
    14521 aatcaaactt caaattggga gaaagagcaa gctttaaagg taacagaaga tttaattcaa
    14581 aagaaaactg attttaatgt tataatatgt caagatgata attctgctac aggtgcaggt
    14641 caagctttaa aagatgctgg tcttaaggat aaagttaaag tcataggttt gggtggaagt
    14701 aaagatggat taaaagctat aactgatggg ttaatagatg gtacaactta tatgtctgct
    14761 gtagaagaag gtggattagt catagaaaag gcatctaaat tccttaaagg tgaaaaaata
    14821 gaacctgtta cacaaattaa acaagttgaa gttaacaaag ataatatttc agagttcaag
    14881 ggtgaatggt aaattgtcat agataataaa taagattttt agagttactt tagaaactat
    14941 ataaagtaac tcttttttta atttaattta gtgataaatt attgaatagt atttctaaac
    15001 aaataaaaaa ttaatataaa caaagactta attatactaa attataagta aaactaatat
    15061 aaacttatgg aaaaaaggaa aacgtctaaa gtattggaat tactatagtg taggtgtggt
    15121 attaaaaaaa cataaaaaaa cataaaaaaa ttgttgaaaa aaatatagaa attttgtata
    15181 attaaagatt tgaaaagtgc ggtatacaaa gccaattctt ttgtatataa tatatataaa
    15241 ttcagaaaaa ggaagggata tgtatggcca ggcggttatt tttagaaagg attggactta
    15301 tatttttagg ttcagcaata ttagcatttg gagtttataa tttttattat ttaaacaaca
    15361 taacagaagg tggagtgtta ggtattttac tactgttaaa aaatttattc aatatacaac
    15421 cagcaatagc aaatgttgta atagatggtt tgctactttt agtaggatat aaatttttcg
    15481 gtaaaaaatt ccttatatat tcaatagtag cttctataac tttttcagtg ttatatgact
    15541 tatttgaagc tataggacct ctagtaccac aatcacaaaa tatgctttta tcaactattt
    15601 tggcaggtgt aactgttgga actggtgtag gtatagtagt aaaagcaggt tgtgcatcag
    15661 gaggagacga tgctttagca ttagtaattt ctaagactac atctttgaac atagggcaag
    15721 tttatttagc aacagatgtt attgtattgt tgttatcatt attttatcta tcagcgtttg
    15781 atatctttta ttctttgatt gctgttacaa ttagtggcaa ggtaattgat tttatttatt
    15841 atcatggtaa aagtttggat atggatataa gtaatgatat agttccagaa tgttaatata
    15901 aattaaataa ctttagattt tattaggggt ggcagatttg ccaccttttt tgtgtctaaa
    15961 tctttataga aagtataatc ctatctaggt ttttgcaaat ctcaaatata gaattatgtt
    16021 gaaatgactg ggaatacgat tgctagatgt gcattttaaa aacccaattt tatatttatg
    16081 aaatactaat cattaatgga tgtttcttaa tcctatcttt atacctaaaa tgataagata
    16141 aatgaagtag caatacaaaa ctaaaatttg taaaagagat taaattaaat ggaggagcta
    16201 aaatgttgga tatagttatg atagcattgt tattcatagg atttgtttcc atgaagttac
    16261 ttgctgattg gtgtgggaaa caaactgaaa aaaaataatg taatgtatgg agggattacc
    16321 tatgtggttt ctggcagttg taattataat gttaataata tatttggtat atgcgcttat
    16381 taatcctgaa aaattttaaa cagtataagg ctaataagta agttgaattg gggggaacaa
    16441 tttttatggt gcagatagta atagtgttag cgatttttat gattttagta atacctatgg
    16501 gcaagtattt atatcatata gctactaatc aaaaaacatt tggagataga ctatttgata
    16561 aagtggacaa ttttatatat aaggtatgta gtatagataa gaaaaaagaa atgaactgga
    16621 aacagtatgc attagcttta ttatttacaa atgcagttat ggtatttata ggatatatca
    16681 tacttagaac tcaaagtatg catattttta atccaagtgg aattaaaagt atggaacaag
    16741 gactatcatt taatacaata ataagtttta tgacaaatac aaatcttcaa cattattcag
    16801 gtgaatcagg actttcatac tttagtcaga tgacagtgat aatatatatg atgtttacat
    16861 cagctgcaac aggatatgcg gctgctatgg catttgtaag aggtctagtc ggtaaaaaga
    16921 aaacattggg aaatttttat gtagatttaa ttaggataac aacaagagta cttttaccag
    16981 gagctttaat tattgggctt atattagtta cacaaggagt accacaaact tttgctggga
    17041 cagaaacagt gactactata gaaggaaagc ttcaagatat agctagaggt cctgtggcag
    17101 ctcttgaatc aataaaacat ttaggaacaa atggtggtgg attctttggt tcaaattcat
    17161 cacacccatt tgaaaatcca acaataatat caaatatagt agaaatatta tctatgatga
    17221 tattaccagg agcttgtgtt gttgcatttg gtcacatgat aaagaataaa aaacaaggtt
    17281 gggttgtgtt tggcgcaatg tcaatcatat ttttaatagg tctagtagtg tgctttaaag
    17341 cagaaagtgc aggaaatcct attttatcac aattagggct taatcaaagt atgggaagta
    17401 tggaaggtaa ggaagttaga tttggtatag cacaatcatc attattcaca acagtgacta
    17461 cttcctttac gacaggtaca gttaataata tgcatgatac actgactcct ttaggaggat
    17521 tagtaccact tttaaatatg atgctaaatg tagtatttgg tggtaaaggc gtaggtctga
    17581 tgaatatgtt gatgtacgct ataatagcag tattcttatg tggattaatg gtaggaagaa
    17641 caccagagtt tttaactaag aaaatcgaag gaaaagaaat gaagcttatt gctttactta
    17701 ttatattaca tccactttta atactaatgt tctctggtct ttcagtagca ataccagcag
    17761 gacttgaagg aatttcaaat cctggattcc atggattatc acaggtattg tatgagtttg
    17821 catcttcagc agccaataat ggttcaggat ttgaaggtct aggagataac actatgtttt
    17881 ggaatataac aacaggtata gttatgttct ttggaagata tgtgtctatt atagtactac
    17941 tagcaatatc aagtttgtta gcatctaaaa aagctgtaaa tgaaagtata ggaacactta
    18001 gaacggataa ttttacattt acaatagttt tagtacttgt tgttttaatt gttggtgctt
    18061 taacattttt cccagcctta gcgcttggac ctatttcgga gcatctagtg ttgtggcact
    18121 aaaaaattgg gagggagtaa agttttatgg aaactaaaaa aacaaagttt attacaaaag
    18181 atattttcaa aacttccatt atagaagctt ttaaaaaatt aaatcctaaa tatatgatga
    18241 aaaatccagt tatgtttgtt gtagaaattg gatttatagt aacattgata cttacaatag
    18301 tacctagttt gtttggtgat gaaggcaata acttaagagc ttataatggg attgtaacaa
    18361 taatcttatt tataacagtc ctttttgcaa attttgctga agctgtagct gaaggaagag
    18421 gtaaagctca agcagataca cttaagaaaa ctagaaaaga tacagtagca agggttatag
    18481 atacgttagg aaatgaaaaa atgataagtg caagtgagct aaaacttggt gatattgttt
    18541 tagtaaatgc tggagaagtt atcccaaatg atggagaagt tatagagggt atagcttctg
    18601 tggatgagtc agcaataact ggagaatctg cacctgtcat gagagaatca ggtggagatt
    18661 tcgcatctgt tacaggtggt actacagttg taagtgactg gttaaagata agaataacaa
    18721 caaaaccagg agaatcgttc ttagataaaa tgatagctct tgtagaaggt gcttcaagac
    18781 aaaaaacgcc aaatgagatt gctttaaata ctttgttagt agggttaaca cttatattta
    18841 tggttgtttt aatatctctt tatccaatgg caaactatgc aggtgtaaag attccaattt
    18901 ctactatgat agctctcttg gtatgtttaa taccaactac tataggtgga ttgttgtcag
    18961 ctattggtat tgcaggtatg gatagagtaa ctagatttaa tgtaattgca atgtcaggaa
    19021 aagcagtaga agcttgtgga gacgttgata ctatgatttt ggataagaca ggtacgatta
    19081 cttatgggaa tagacttgca tcagaattta tagcagttgg aaatgcagat tcaaaagatt
    19141 taataaatta ttctgttatg tgttcattaa aagatgatac accagaagga aaatctatag
    19201 ttgatttagg tatgaaatta ggttcaacag gtaagacaaa agaggcagag gaagctgagt
    19261 ttgtagaatt ttctgcacaa actagaatga gtggtattaa cttagctgat ggtagagcta
    19321 taagaaaagg tgcttatgat agcataataa aaagagtaaa agaatctggt ggtacagtgc
    19381 cagaagattt agaagaaaat gtaaatcgag tagctaaact aggagggaca cctttagtag
    19441 tgtgtgtaga taatgagata tatggaatta tctacttaaa agatacagta aaatcagggc
    19501 ttgtagaaag atttgctaga cttcgagaga taggaataaa gacaataatg tgtacagggg
    19561 ataatccctt gacagcagct acaattgcaa gagaagctgg agtagatgga tttattgcag
    19621 aatgtaaacc agaagataaa atagatgcaa taaaaaaaga acaattagag ggtaaaatag
    19681 ttgcaatgac aggtgatgga acaaatgatg ctcctgcact tgctcaggca gatgtaggtc
    19741 ttgcaatgaa tagtggtaca acatctgcaa aagaagcagc aaatatggtg gatttagatt
    19801 cagacccaac taagatattg gaagttgttg aaataggaaa acagctttta ataactcgtg
    19861 gggctttgac aacatttagt atagcaaatg atgttgcaaa atattttgcg attataccag
    19921 ctatatttac tcttgcaata cctcaaatgg agatattaaa tgtaatgaaa ctttctacgt
    19981 catttagtgc gatattatca gctttaatat ttaatgctat aattatccca tgtttaatac
    20041 ctcttgcaat gaagggagta aaatatagac ctatgaaatc acaagctatg ttaatgagaa
    20101 atatgcttat atttggtctt ggtggagtta tagtaccatt tttaggaata aaattaattg
    20161 atttattgat tacaccttta gtaatggttc ttaatttagg ttaataaatg gaggatataa
    20221 aaatgaaaac attaaagcca gcattacttg taagtattgt attgttagtt gtttgtggac
    20281 ttgtatatcc acttgttttg acaggtgtaa gccaagttgc atttaaagat aaagctaatg
    20341 gaagtatgat agaagtaaat ggtgtaaaag taggttcaga acttattggt caaagcttta
    20401 cagatgcgag attctttaag ggaagagtat catcagtaaa ctataatact tatacaaaag
    20461 aagatttagt accagataaa gatggaaaca catcttatgg aggtgtatca tcagggtctt
    20521 ttaattatgg agctacaaat ccagaacttc atgatagagt acaaaaagat atagaacagt
    20581 ttttaaagga taatccaact gtcaaaagag aggatatacc aacagattta cttacagcat
    20641 caggttcagg acttgaccct aatataagtc cagcctctgc aaaaattcag ataccagcta
    20701 tagcaaaagc gtctggaata tcagaaagta aattacaaaa aattgtagaa gataatactt
    20761 ccaaaaaact ttttggagtt ttaggtgaag atagagttaa tgtattaaag gttaatgtag
    20821 aggtcgcaaa aatattaggt cttatatagt attttgtgga attttagcaa caaaatatta
    20881 ctcaagattt aatttaaagg ttaaatttta aagagtgaaa atcgataatg attttcactt
    20941 ttttatttga atattactaa tatttggaat aaagtgattg taagtataaa atccaaaaaa
    21001 aggttgcatt atcaaaatga taatactata atatatttat cgttatcaat ttgataatag
    21061 atttggacat ataggaggaa atagtatgtt atataataac gcattagagt tgataggaaa
    21121 cacacctgta gtaaggctga ataatttagg ctgtactaat gggtgttcta acatatatgt
    21181 aaaattagaa aaagtaaatc cagcaggaag tataaaagat agagctgttt atgaaatgtt
    21241 agagggttta gagcaaagag gagaattaaa aaagggagat gtactagttg aagcaactag
    21301 tggaaatact ggtattgcat tatctatgat aggaaaatta aaaggatatg aagtagtaat
    21361 cgtaatgccc gaaacaatga gcgttgagag aagagattta atgaaggctt atggagcaaa
    21421 tttggtgctt acagatggta aagctggaat ggcaggttcc atagaaaaag caaatgaact
    21481 tttaaaagaa aatccaaact ataaaagctt aaaacaattt gaaaataaag ataatccaaa
    21541 tgcacattat aaaacaacag cagttgaaat tatgaatgat gtcaaagatt tagatatatt
    21601 cgtatgtgga gttggaacag gtggtacttt aataggaact gcaagatatt taaaagaaca
    21661 aaatccaaat ataagagtaa ttgcacttga accaaaaaaa tctccagcta tatcagaaaa
    21721 caagactgga cctcataaaa tacaaggaat aggtgctaac tttgttcctg aaaattatga
    21781 ttctagtgta gttgatgaag taatattagt agatgatgaa gatgcatttg atacagttaa
    21841 attacttgca gcaaaagagg gaattttagt agggatatct tctggagcaa atatatttgg
    21901 tgctatagag atggcaaaaa agtatccaga taaaaagata gttacagtag ctccagatgg
    21961 agttgataag tatatgtcta tgggaatatt ttaataattt tatttgaggg agtgaatact
    22021 ttgtttaaga aaataaataa agatattgaa tatataatga agaatgatcc agcagctagg
    22081 tctaagatag aagttttttt actttatcca tcagttcatg caatgataat gcataggatg
    22141 gctcatgctc tttataagaa gaagaagctt tttacagcaa gattaatatc ccagatatcc
    22201 agatttatga ctggcataga aatacatcct ggtgctaaaa tgggggaagg tatattgata
    22261 gaccatggaa tgggtgttgt aattggagag acagctgaag taggtaatag ggtgacaata
    22321 tatcaaggag ctactttagg agctacagga aaagatactg gaaagagaca tccaactgtt
    22381 ggtgatgatg tattaattgg tgctggaaca aaaatactag gacctttaaa tataggttca
    22441 aattcaaaaa ttggtgcaaa ttctgttgtc gttaaggatg tacctaatgg tgcaacagtt
    22501 gttggtatac cagcaaaaat agtaaaaata agaaacctag aacctgtaaa aaagaataaa
    22561 aaagaagtgt cctatgagta tgatgaatta gataatgtat attatatata ggatttatat
    22621 aactataaaa taaactttat aaaaattatt ttaaaaggta tttttttcaa atttgaggac
    22681 ataataaaaa agaactgaat gttacagttt tggttcatca atctttgata aacaagattt
    22741 taaatttgta gcgtctagga ggattttatt atgaaagcaa atgttaatca agatacttgt
    22801 ataggttgtg gattatgtcc atcaatatgt ccagaagtat ttgatatgaa ggatgatggg
    22861 aaatcacatg ttatagttga tgaagtacca agtgatgctg aagaatcagc agcagaagct
    22921 agagaaagct gtccagttga tgcaatagat gtgcattaat aaaataaatg cctataaaaa
    22981 ttgaattttc aatttttata ggcatttgtc ttttattcag ttagtatttc aacacctgtt
    23041 tctgtaacta aaaccataca ttcccattga gcagataatt taccatcttt tgtataaata
    23101 gtccaattat tttcttcatc aacaaaaatt tcgtgagttc cttcatttac cataggttct
    23161 attgtgaata tcatcccagg aaccaatagc atctcagtcc cttttttaga tacataactt
    23221 acaaaagggt cttcatgaaa gcttaatcca actccatgac cacctatttc tctaacaaca
    23281 gagtatccat ttttctttgc ataagagttg ataacatcac ctatatctcc taagaatccc
    23341 catggtttgg caacttcaag acctttttgg acacattcct ttgtaacttg aactaatttt
    23401 tttgcatttt cgttaacatt acctatcata aacattcttg aagcatctga atagtatccg
    23461 ttaaaaatag tagaaacatc tacatttata atatctccat cttttaagat aatgtcttta
    23521 cttggaatac catgacaaac ttcattattt actgatgtac atacactttt aggaaatcca
    23581 ttatagtcta gtggagcagg gatagctcca ttttgtagtg taaagtcatg aaccaactta
    23641 tttatatctt ctgtagacat tccaatttcg attttatctg aaatataatc caaaattttt
    23701 gtgtttatta cagcactttt ttttatttcc tcaatttgac tttcattttt tataatatca
    23761 tgagttggta caatatgacc taacttagca aatgaatcta ttttattatc catatccata
    23821 tgacattttt tatatttttt agtacttcca caccaacagc tatcatttct atttaacttc
    23881 ataacttttc atccttcttt tattatttat ttaaattatg ggttacataa actattttaa
    23941 aacataacca ttataaatat tataacataa atactaagaa atcctttata gatagtgatt
    24001 tattcaaagt ataaattatt atatggttat tgttgatgaa aagaggatag ttaaatgtat
    24061 tattggatat tagcgcactt aatagtttag ttcaagtttt tcaagaaaat catatgttag
    24121 aggacaattt tcctttaaag tattttttga atcttcatca agataataat gtacaaatga
    24181 ttctgcaaaa aattcttcta taaacttgct tatatatgca tgtgcaactt gcttagggtc
    24241 ataaagctta tatctttcct gagcaaatac ttccataaat tcttcagaat ttgatttttt
    24301 atttaaaaca tttttatcta tagcatgtgc agtttcatgt aattctaaat tttttgatga
    24361 atgtacattt gcatagctag ggttactata acctatttta gccactatag aattatttct
    24421 atatatacca ggtacatctt tccaagtctt gcctgatctt acccaacatg gaggtaattg
    24481 atattttaaa tcactaaatt ctggttcatc agttagattt gaatttatta attttatttt
    24541 tatatccttt tctttaagtg tctgtaaata tttaatattt atttttgcaa gtctgttttt
    24601 catattttca acttcatctt tatcataatt caagtcatca ggaagtataa gcatttgttc
    24661 aattatatct atactactgt cttttatatt ttcagattct tcatcaaatt tatattgaag
    24721 agtattattt tcatagatag ttatatttgt attattttct atagaataag agtgaatatc
    24781 ctctaaatct aaaatattca tttgttgtaa aatatgtttt gtttcgccac tccacactac
    24841 taaagaaagt gaattaaaat taattagtgg attttctttt gcttttatat cattgcttat
    24901 atctacatca aatacatatt caattgagtc ttctatgtta agactatctg tattcttttt
    24961 aatatataaa tacgcttgtt ttaagtctat ataaatctca tctaaagatt cgtcataaaa
    25021 ttctgataaa ttattagtaa agagttcttc acttaaaagt tcatttttat cagttatact
    25081 ttcaattaaa atattaattt cttttatcat attgtaaata cttatttttc tatttgcttt
    25141 ttcactagat acattgcatg gcgcaagagc aatgcaataa aaatcttttt cttttttata
    25201 aaaggcttta agtgattttg ataaaagtgt aaagtctaca cctatctgta agttgacctt
    25261 aagtgagtca ttttgtaata attcagtatt atttctttta gctgtattta aattgttttc
    25321 cataatcatt gccccctttt tattagggta tcataatttt tttatatgtg tcaatttgtt
    25381 tttttgaaaa ttataatttt tttgaaaatt ttatttttta taatttgtga attttt
//
LOCUS       DBGJFU010000047        25201 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.047, whole genome shotgun sequence.
ACCESSION   DBGJFU010000047 DBGJFU010000000
VERSION     DBGJFU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.047"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            67..1470
                     /locus_tag="ACOIAS_001875"
     CDS             67..1470
                     /locus_tag="ACOIAS_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701587.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            1497..3041
                     /locus_tag="ACOIAS_001876"
     CDS             1497..3041
                     /locus_tag="ACOIAS_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HGZ2701588.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKSGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            3072..4463
                     /locus_tag="ACOIAS_001877"
     CDS             3072..4463
                     /locus_tag="ACOIAS_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGZ2701589.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            4656..6152
                     /gene="abfD"
                     /locus_tag="ACOIAS_001878"
     CDS             4656..6152
                     /gene="abfD"
                     /locus_tag="ACOIAS_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HGZ2701590.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            6287..6562
                     /locus_tag="ACOIAS_001879"
     CDS             6287..6562
                     /locus_tag="ACOIAS_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701591.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            6586..7893
                     /locus_tag="ACOIAS_001880"
     CDS             6586..7893
                     /locus_tag="ACOIAS_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HGZ2701592.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            7913..9031
                     /locus_tag="ACOIAS_001881"
     CDS             7913..9031
                     /locus_tag="ACOIAS_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HGZ2701593.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            9318..10238
                     /locus_tag="ACOIAS_001882"
     CDS             9318..10238
                     /locus_tag="ACOIAS_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HGZ2701594.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            10353..11510
                     /locus_tag="ACOIAS_001883"
     CDS             10353..11510
                     /locus_tag="ACOIAS_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HGZ2701595.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(11591..12487)
                     /locus_tag="ACOIAS_001884"
     CDS             complement(11591..12487)
                     /locus_tag="ACOIAS_001884"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGZ2701596.1"
                     /translation="MLKKVKYPIGQVKVSDKFKTIKLLSRKYSISSMCKLISVSRSGY
                     YKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYRGTYGRKRICTYLNKILDTPINHKRVY
                     RLMKELGIKSIIRKKVYRRKFKSYEIYDNILNREFRANQPLEKICMDITYIPIGKKFL
                     YMNVAKDLFNGEIVAYEISTKMDTKLVNNTVNQLINMNLAKDCILHTDQGSQYTSRSY
                     SKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKEMISLINDYIYF
                     YNNERIQLKNGMSPIEYRTHSE"
     gene            complement(12445..12762)
                     /locus_tag="ACOIAS_001885"
     CDS             complement(12445..12762)
                     /locus_tag="ACOIAS_001885"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGZ2701597.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFDDETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            complement(12899..14398)
                     /locus_tag="ACOIAS_001886"
     CDS             complement(12899..14398)
                     /locus_tag="ACOIAS_001886"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGZ2701598.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            14994..16418
                     /locus_tag="ACOIAS_001887"
     CDS             14994..16418
                     /locus_tag="ACOIAS_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGZ2701599.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            16444..18582
                     /locus_tag="ACOIAS_001888"
     CDS             16444..18582
                     /locus_tag="ACOIAS_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2701600.1"
                     /translation="MDNLKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSR
                     TVLRDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEER
                     NLIILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFSLASKWLERFDIELVRKPGLGVY
                     LKGQEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCI
                     IIKVEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITE
                     EFKLAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITN
                     KMIDLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYD
                     GVEKISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSR
                     FLSTKIENKFPNLNILETISAINIDEEYLKGKDVDLIVSTVELNTSLNYICVGPFMSL
                     DDEQIIKEKIKSIAQNKLINLNTKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEK
                     FESKDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERID
                     IMKLSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKI
                     NKFSIEEMTLEVKDIVFNFYTNRLKAFIDE"
     gene            18612..19034
                     /locus_tag="ACOIAS_001889"
     CDS             18612..19034
                     /locus_tag="ACOIAS_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2701601.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAK"
     gene            19053..20204
                     /locus_tag="ACOIAS_001890"
     CDS             19053..20204
                     /locus_tag="ACOIAS_001890"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGZ2701602.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      20310..20411
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            20594..21166
                     /locus_tag="ACOIAS_001891"
     CDS             20594..21166
                     /locus_tag="ACOIAS_001891"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGZ2701603.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            21230..21880
                     /gene="fsa"
                     /locus_tag="ACOIAS_001892"
     CDS             21230..21880
                     /gene="fsa"
                     /locus_tag="ACOIAS_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGZ2701604.1"
                     /translation="MIFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHEGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            22032..24956
                     /locus_tag="ACOIAS_001893"
     CDS             22032..24956
                     /locus_tag="ACOIAS_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2701605.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPVLNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         9865 a         2842 c         4627 g         7867 t
ORIGIN      
        1 aatagagtat tggggagaaa ggagaaatat attttttaat aatatttgtt aatttaataa
       61 cgaaaaatga aagggagtga tttagtgaag tttgaattga tggatttttt aatgaatcct
      121 tttgtcctta tgtttgctgc agtaattaca ggaattttat ttggaaaaat taaatttggg
      181 aagtttaatt ttggggtatc aggagcatta tttacaggat tatttattgg atggcttgca
      241 tatagcttgg ggaatctaat aatagcaaaa ggggaaacag cagcaggata taaggcagca
      301 actgtaatga tggggaatgg aattatttct tctgacttct ttgatttctt tttaataata
      361 tttgttgcag cagttggtct attggcagca aaagatatga aagctgtatt aaagaagtat
      421 ggagcaagat ttgtaatttt aggagttctt ataactttta taggtggctc tatgacttat
      481 gcaatgactt tactatcaag tgataaagga agtagtgctt atgaagtttc tggcgtttac
      541 acaggagcat taacaagttc gcctggatta gctgctgctt tagaaacagc tggtaaacat
      601 gcagaagatg tatcaaaaga atttgaaaaa gcttcaataa aagataaaaa agaaatttta
      661 aaagtagtcg acccagaagg caaattggat gtgaatacaa ctacatcact gactcaagag
      721 caaatagata agtatattgc atatgctgaa gctggtgttg gtataggtca tgcagtcgca
      781 tatccttttg gagttttaat agtaatctta ggtgtaaatt tccttccaaa attatttagg
      841 atggacctaa aagaagaaag aagaaaatat gagaaggaaa tgaaggaagc aagagatagt
      901 gtttctggta aaacgatacc agaagttcca tttaatataa taacattttt cttgacatgt
      961 ttagctggat acttagttgg aggaatacat gtatttatgg gaccactagg gtacttcaca
     1021 ctaggtgcaa caggaggttc actaattgta tctctagttc ttggatatat aggtaagata
     1081 ggggtagtaa atttcagaat ggaagaaaag gttttaaata ttttaaaaca aatagggcta
     1141 gtgtttttct tagcaatagt tggattaaga tatggtggaa aagtagttga ttcaattatg
     1201 acatctggta tgcacctagc cttagtagca atagcagtag gtgttacagc tatgatgata
     1261 ggattcttag ttggcaaata cgtatttaaa ttaaattgga ttttattatc tggagctgtc
     1321 tgtggtggta tgacatcaac accaggtctt ggagctgctg ttgatgctct tgatagtgat
     1381 gacccagcag caggttatgg tgctacgtat ccatttgcat tattaactaa agttatattg
     1441 gttatagttt tacacaaact tccaatgtaa ataagatttt atatgggggg atatttatga
     1501 ataatgaagg agcatttgaa gaattaagtg gaagattaag aaataaagaa ttgtgtgatt
     1561 taataatgaa tgcagatgaa gctgctaaaa ttatagagga tggcatggtt attggagtta
     1621 gtggatttac accttcaggc tatccaaaag cagtaccatt agcagtttca gaaagagcta
     1681 aaagtggaga aaagataaaa ttaaccgtat attcaggagc atctttaggt ccagaagttg
     1741 atggagcatg gtcagaagca ggtataattg aaagaagact tccttatcaa actaattcaa
     1801 tacttagaaa taatattaat aaaggtgttg ttgattatat agatatgcac ctaagtcatt
     1861 ctactcaatt tttaaattat ggaactatac ctaaagtaga tgtagctata gtagaagcac
     1921 ttgcaataac tgaagaaggg aatattatac caactagtgg gataggtaat tctccatctt
     1981 ttataaaaag tgcagataaa gtaatagtag agataaatct tgcaaaacca atggaaatgg
     2041 aaggtatggc agatatatat attacggaaa atccaccaaa tagaaaacca atagaaataa
     2101 atcatcctaa ggatagaatt ggaacaacat atataccttg tggtttggat aaaattgcag
     2161 gagtagtaat taccaatatg caagataaga cccgtccatt gggggttgta gatgaagctt
     2221 cgaaaaaaat atcaaataat attattgctt ttttaagaga agaagtaaag tcaggcagat
     2281 tatcaaagaa tttacttcct cttcaatctg gtgtaggaag tgtagcaaat gcagtattat
     2341 atggattatg tgaatctgaa tttgaaaatt taacttgtta tacagaggtt gtacaggact
     2401 ctatgttgga tttaataaga atgggtaaag ttacaatggc atctactaca tctgtaagtc
     2461 catctccaga aggattaata aaatttgaaa aagatattga tttttttaag gataagatta
     2521 tattaagacc tcaagaaatt agtaacaatc cagaaatagc cagaagaatt ggtgtaattg
     2581 caatgaacac tgcaattgaa gttgatattt atggaaatgt aaattctact cacataatgg
     2641 ggtcaaagat gatgaatgga attggtggtt ctggtgattt tgctagaaat ggagcaataa
     2701 ctatatttag tactgagtca attgcaaaaa atggtgatat atcatcaata gtacctatgg
     2761 tatctcacgt agaccacact gaacatgatg taatggtaat tgtaacagaa caaggatatg
     2821 ctgatttaag aggtcttgca cctagagaaa gagctataaa aataatagaa aattgtgccc
     2881 atccagatta taaagaacaa cttagggatt atttaaatag agcttgtaaa agtggagcaa
     2941 aacaaacacc tcacatattg gacgaagctt tatcatggca tagtaaattt atgagtacag
     3001 gtacaatgaa aaaggctgaa acttttaaaa gtgcattata aaatatttaa taaattggag
     3061 gtagatttta aatggaaaaa gcagtggaaa actttgaaga tttaagtaag gaatacatta
     3121 atggttatat tgagagagca agaaaagctc aaagagagtt tgaatgttat actcaagaac
     3181 aagtagataa aatagtaaaa atagttggaa aagtagtgta ttataatgct gaatatttag
     3241 ctaaacttgc tgtagaagag actggaatgg gagtatatga agataaggta gctaaaaata
     3301 aaagcaaagc aaaagttata tataacaatt taaaagataa aaaatcagtt ggtataattg
     3361 atatagatag agaaactgga ataacaaagg tagctaaacc agttggagta gtagcggcaa
     3421 taactccttg tacaaatcca atcgttacac ctatgagcaa tgcaatgttt gcacttaagg
     3481 gaagaaatgc aattattatc acacctcatc ataaagcaat tggatgtagt acaaaaactg
     3541 tagagatgat taatgaagaa ttagaaaaaa taggagctcc agaaaattta atacaaatac
     3601 ttgaccaaca atcaagagaa aatacaagaa atttaatttc atcagcagat gtagtaattg
     3661 ccactggagg aatgggaatg gtaaaagctg cgtactcaag tggaaaacca gcacttggtg
     3721 taggggcagg aaatgttcaa tgtatcatag atagagatgt agacattaaa gaagcagtac
     3781 ctaaaatcat agcaggaaga atttttgata atggtatcat ttgttcaggt gaacagtcag
     3841 taatagtagc agaagaaatg tttgataaaa taatggatga atttaaaaat aataaaggtt
     3901 ttatagttag agataaagta caaaaagaag ctttcagaaa tgctatgttt gtaaataaat
     3961 ctatgaataa agatgcagta ggtcaatcag ttcatacaat tgcaaaaatt gcaggtgttg
     4021 agataccaga agatacaaaa ataatagtaa tagaagctga tggaccagga gaagaggata
     4081 ttattgcaaa agaaaaaatg tgtccagtta tatctgcata caagtataaa agttttgaag
     4141 aaggtgtagc tattgcaaaa gcaaatttaa atgtagaagg aaaaggacat agtgtatcta
     4201 tacattcaaa tacagttaaa aatatagaat atgcaggaga aaatatagaa gtatcaagat
     4261 ttgttattaa tcaatgttgt gctactagtg ctggaggaag tttctttaat ggtcttgcac
     4321 ctactaatac tctaggttgt ggttcttggg gaaataacag catctctgaa aatcttgatt
     4381 ataagcatct aattaatatt tctagaattg cttattatat gccagaaaat gaagtcccta
     4441 cagatgaaga actttgggga taaaatttag acaaaaaata ttcattctaa attaaagtag
     4501 gaatatattt taatttagaa tgaatttaag acataaatta aatttaaatt agtttattgg
     4561 actaaattta aaatattaag agacatatag aagaaggaag tattataatt taaaataaat
     4621 aaaaaaatct ataaaagaga aggagtggtt ttattatggc attaatgaca ggagctcaat
     4681 atattgaaag tttgagaaaa ttaaatacta aggtttatat gtttggtgaa gaggttaaga
     4741 attgggttga ccatcctatg attagaccat ctataaattg tgtagcagca acttatgact
     4801 tagctcatga cccagaatat gcagatttaa tgacagttac ttcaaatatc acaggtgaaa
     4861 aaataaatag atttggtcat cttcatcaaa gtgttgatga tttgataaaa aaagtaaaaa
     4921 tgcaaagact atgtggtcaa aaaacagctt cttgtttcca aagatgtgtt ggtatggatg
     4981 cgtttaatgc agtgtattca acaacatttg agtgtgataa agcccatgga actaattatc
     5041 atgataattt tgtaaaatac ttaacttata tacaagaaaa cgatttagta gttgatggag
     5101 ctatgactga ccctaaggga gatagaagtc tatcacctag tgctcaacct gacccagata
     5161 tgttccttca tatagtagaa agaagagaag atgggataat tgtaagagga gcaaaggcac
     5221 atcaaactgg ttcaataaat tcacatgagc accttataat gcctactata agtatgactg
     5281 aagcagataa agactacgct gtatcatttg cagttccttc agatgctgaa ggagtgttta
     5341 tgatttatgg aagacaatct tgtgacacta gaaaattaga agaaggtgca gatgttgatt
     5401 taggaaataa ggagtttgga ggtcaagaag ctttagtagt atttgataat gtatttgttc
     5461 caaatgatag aattttctta tgtggagaat gggatttctc tggtatgtta gtagagagat
     5521 ttgcaggata ccatagacaa agttatggtg gatgtaaagt aggagtaggt gatgtaataa
     5581 taggagcagc agctttagca gctgattata atggagcaaa caaagcttca catataaaag
     5641 ataaactaat agaaatgact caccttaatg aaagcttata ttgctgtggt atagcatgtt
     5701 catcagaagg tcacaaaaca gaagctggaa actatcaaat agatttactt cttgcaaatg
     5761 tgtgtaaaca aaatgtaact agatttcctt atgaaatagt aagacttgca gaagatatag
     5821 ctggagggct tatggttact atgccttcag aaaaagactt taaatcagac cttaaagttg
     5881 gaacaagtgg aatgactatt ggcgaagtat gtaacaagta ttttaaagct tcttcagtgg
     5941 cttcaacaga agaaagaatg agaatattaa gattcttaga aaatatatgc cttggttcat
     6001 ctgctgttgg ttatagaact gagtcaatgc atggggcagg ttcaccacaa gctcaaagaa
     6061 taatgatttc aagacaagga aacataaatc aaaagaaaga attagctaag aaaattgcgg
     6121 gaataaaaaa agaggaagct ttaaatttat aaaaagttga tatataaagg gaaattatat
     6181 attaatgtgt attaattaat gaaaaaatat gtagtataag agatagttat aaaattatgg
     6241 tggtttttag ctaagtaaag aaatttttaa taaggagaat ttacacatga cctgtggagt
     6301 tagattttgt gggggctgta accctagata cgaaagaggg aaggctctta caataatcaa
     6361 agagcacttc aatggtaggg tagattttag tatagcacaa gagaacgtcg agtatgattt
     6421 gctacttata attggaggat gcgcaactct atgcgcatcc tatcaacaat ataaagtgaa
     6481 aaatgaatgt gttaagatgt gggattttag ccatatagaa agagttataa atgtgattga
     6541 atcagcaatt tgtgaaactt aaaaaagcta aatgggggga tttcattgag ttggcaagaa
     6601 ttgtatcaaa gtaaattatg tagtgctaca gaagctgtaa aacagattaa aaacggtgat
     6661 actgtagtat ttgcacattg tgtaggtgaa cctcctgcac tagtagaggc tatgattgaa
     6721 aatgctgaac aatataaaga tgttgagatt aaacatatgg taagcttggg tagtggtgga
     6781 tatactgcta agggaatgga agctcatttt agagtaaatc caatgtttgt aagtggaaat
     6841 gtaagaaagg caattgaaaa tggagatggt gattttacac ctgcattctt ccatgaagta
     6901 ccaaagttgc ttagagaaaa aagattaaaa tgtgatgttg ttttagcaca ggtaactcca
     6961 ccagatgaac atgggtattg tagcttagga acatcagttg attatactta tgaagccatt
     7021 aaaactgcta gaacagtaat tgttcaagtg aatgaccagt ttcctagaac ctatggtgag
     7081 gtagtccatg tatcagagtt tgactatata gtggaaaaat cacaaccttt atttgaacta
     7141 caacctgcaa agattggaga agtagaagaa gcaattggta aaaattgtgc ttcattaatt
     7201 gaagatggtt ctactttaca gctagggatt ggtgggatac cagatgcagt tatgttattt
     7261 ttaactgata aaaaggattt agggattcat tcagaaatga tttcagatgg gactttggcc
     7321 ctttatgaaa aaggtgttat aaatggtaaa tataaaaatt ttgataaaga aaaaatgaca
     7381 gttaccttct taatgggtac taaaaaatta tatgactttg caaataataa ccctgcagta
     7441 gaggtaaaac cagtagacta tgtgaatcat ccagcaatta taatgaaaca acataagatg
     7501 gtttctataa attctgctat acaagttgat ttaatgggac aggtagttgc agaggctatg
     7561 ggacttagac aatttagtgg tgttggaggt caagttgact ttatacgtgg agtgtctatg
     7621 ggtgaagatg gaaaggcaat tatagctatg ccttcaataa ctacgaaaaa agatggtaca
     7681 gtaatttcaa aaatcgtgtc tattgtggat gaaggtgcac ctataacgac atcaagaaat
     7741 gatgttgact atattgtaac agaatacggt atagcagaat taaaaggaaa atctcttaga
     7801 gaaagagcga gaaatttaat aaatatagct catccaagtg taagagaaag tctagcagta
     7861 gaatttgaaa agagatttaa ggagaaatat taataataga ggggtgttaa taatggaagc
     7921 acttagagta gttccaaaga tattttactt tgatacattt aaagaattta atgaagaatt
     7981 taaaatagga aaaaatgatt tagtaattac taatgagttt atatatgagc catatatgaa
     8041 acctcttgga atagatacaa atttaatttt tcaagaaaag tttggaacag gtgaaccatc
     8101 tgatgaaatg atagactcca tgacaaagga aatgaagaaa tacaattttg atagaattat
     8161 tgcatttgga ggaggaacaa tagttgatat atgtaagata ttagcacttg atgttccaga
     8221 aaagtctatt gatttatttg aaggagaagt atcacctaaa aaagtaaaag agttagtagt
     8281 agttcctaca acatgtggta caggtagtga agtaacaaat gtagctattg cagagttaaa
     8341 atctaaacat actaaaaagg ggcttgcagt agaagaaaca tatgcagact atgcagtgtt
     8401 aattccagag acagtaaaag ggttacctta caaattcttt gttacaagtt cggtagatgc
     8461 tcttatacat gctattgagt cttatctttc tccaaaagca agtccattta cagagatgta
     8521 ttctctacaa gcaataaaaa tgattatgga tgggtataag aagatagttg ataaaggaga
     8581 agaagagaga tttaatcatc ttagagactt tgtacttgca tcaaattatg caggaattgc
     8641 ttttggaaat gctggttgtg cagcagttca tgcactatct tattcaatag gtggagcttt
     8701 ccatgttgca catggtgaag caaattatca gttctttact gaagtattta agatgtattc
     8761 tagaaaaaaa cctaatggaa aaattataaa atgtacaaaa attttagcag atgcccttga
     8821 atgtgaccct aactgtgatg tttatggaga attagaaaag tttttaaata aattaatagc
     8881 taaaaaagct ttgagagaat atggaatggt ggaaagtcag atagatgaat ttacagatag
     8941 tacaatagca aatcaacaac gactacttgc taataattat gtagagctta gtagagagga
     9001 aataagagag atatttgcaa atctttatta atacaatttt ttatttatat gtatagtcaa
     9061 cataaacaat gctaattata tttatgtaca aataaaaata agggaactta aattttaatt
     9121 aaagttaagt tctcttattt ttatatggag ttaaatataa gtatattttt aatttaaaat
     9181 atgcttatat ttaagtctat tactttattt gaaacaattt gtagaatttc aaattccttg
     9241 acagtaataa gtaagtaatt gtataatcaa actgcaatat attaataata ttagtgaaaa
     9301 tgaaaggatt ataaaatatg tataaatcag aaaatttttc aaaaaaatct gtattaataa
     9361 attcattttt aagtaaattt ttaaaaataa ttggatgggg tttggtaagc ttatttacat
     9421 tatcagcctt agtactttca actatttcca ttataattgg atgggctgta atacttccta
     9481 ttcaagtatg tgcagtattt ttacttatag gaggagtttg tatttttaaa ggtaagagat
     9541 taggagacca aatttctcgt tataaaaaat attgtacaat tataaacaat agaaatatta
     9601 ttccagtaga acttattgca gaaaacacat ctaaatcatt aaattttata ataaaagatg
     9661 tacaaaagat gatagacaaa aactacttta taaatactta tattgataaa agaaataatc
     9721 agattgtgtt aacaaatgaa gattttgttc caccaagata tgaagatgta gcatatgaag
     9781 tcaaagaaga agaaaagact gatgaatcca atgaagttga tacaattata aataaaggaa
     9841 tgaattatct aacacaaata aaagaagcta ataaaaatat taaatcagaa tcaatgggcg
     9901 ataaaattgt acaagtagaa gatgttacaa gtaaaatatt tgatgttgta aaacatgacc
     9961 catcaaaact tactcaaata caaaaattta tggattatta tcttccaacc actttaaaac
    10021 tattaaattc atatcataca ttggaagaac aaggaattga tagagaaaat ataacaacaa
    10081 ctatggaaag tattgaaaat acaatgaata cgattgtagt agcttttgaa aatcaattgg
    10141 actatctgtt tgaagatgaa gccattgata tttctacaga tattactgtt ttagagaata
    10201 tgttagtcca agaaggatta acgagtggta aattttaact tcaatagaaa aaatattctg
    10261 ttatttttaa acaattaatt agtcataaag tataaaatgt gtattatata agttaatcag
    10321 tactttatga aggaagagga gagaaagata caatgaataa tttagatgat ataccagtta
    10381 tgccaacttt gacacttgac ccatttggag aagaatcaaa cactaatgat atagataatt
    10441 ctgacttatt aatgaaaaag gatgaaaaag acccagaaga agaaaaactg tctgaatctg
    10501 aacgcaagat ggtaaaggaa tttgcggaaa aaatagatat tacaaataca aatatgattt
    10561 tgcaatatgg agctggtgct caaaaaaagg tagcaagttt ttctgaaaca gctttaaaga
    10621 gtgtaaagac aagagattta ggagaagttg gagatatgct tacaaatcta gttactgatt
    10681 taaaagcatt ttcagcagat gaaaaagaac aaagtggttt tttaggaatt tttaaaaagg
    10741 caaataataa aatttcaaat ttaaaaacta agtatgatag tgcagaggta aatgtggata
    10801 aagtaagcaa agagcttcaa aagcatcagg ttaaaatatt gaaagatata gcaatgcttg
    10861 ataagatgta tgaattgaac ttagcttact caaaagagct tactatgtat attatagcag
    10921 gaaaacaaaa attaaaggat atgaaagaat atgagatgcc aaagttgaga gaaaaagctc
    10981 gtttatctgg ttcaacagaa gatgctcaat ctgtaaatga tatggtttca ctttgtgaca
    11041 gatttgaaaa gaagattcat gacttagaat taactagaat ggtatcgctt caaatggctc
    11101 ctcagataag attggttcaa aatagtaata atctgatggc agaaaagatt caatcaacta
    11161 tagtaaatac cattccatta tggaaaaatc aaatagtgct tgctcttgga atttctcact
    11221 taaatcaagc tatgaaagca cagcgtgaag ttagtaatat gacaaatgag cttttattaa
    11281 agaatgcaga aactttgaag atgggaacaa tagaaacagc aaaagaatca gaaagaggaa
    11341 ttgtagatat agacacaatt aagaaaacta atcaatctct aatatctact attgatgagg
    11401 tagttaagat acaacatgat ggtagaataa agagacaaga agcagaagtt gaattatcaa
    11461 aaatagaaaa tgaacttaaa agcaagcttc ttgaatttac agttaaataa aaattatact
    11521 aagataaaaa gagactgtct caaaattgga tatgtccctg tcaagtagac agacttaaaa
    11581 aaggctatct ttactcggaa tgagttcgat attcaatcgg actcattccg ttttttaatt
    11641 gtattctttc attattataa aaataaatat aatcatttat tagactaatc atttcctttg
    11701 gatctgtagt atcaattaaa tatattaatt cagatttaaa atgactgaaa aaactttcta
    11761 taggagcatt atcccagcag ttgccccttc gggacattga ttgtataata ccattatcct
    11821 taagcctttt agagtaagat ctacttgtat attgtgaacc ttgatctgta tgtaaaatac
    11881 aatctttagc taaattcata tttattagtt gatttactgt attattaact aatttagtat
    11941 ccatttttgt acttatctca tatgccacta tttcaccatt aaataaatct ttggctacat
    12001 tcatatataa gaatttttta ccgataggta tgtatgttat atccatacaa attttttcta
    12061 atggttgatt agctctaaat tcacgattta atatattatc atatatctca tatgatttaa
    12121 acttacgtct atatactttc tttctaataa tagatttaat acctagttct ttcatcaacc
    12181 tatatactct cttatgatta ataggggtat caagtatttt atttaaataa gtacaaattc
    12241 tttttctacc ataagttcct ctatattttt tatgtatttc aattatataa tccttaataa
    12301 ttctatcttt gataccccta tcggaagatt ttttagaata acttagccat ttatagtaac
    12361 cgcttcttga cacacttatc aacttacaca tactagaaat actatacttc ctagacagaa
    12421 gtttaattgt tttaaattta tcgcttactt tgacttgtcc aatagggtac ttaacttttt
    12481 taagaattca ttctccatgc gtaagtactt caattcttct tcaacagatt taaattttct
    12541 attatcattt tctagactaa tatttttaaa tctgccccta gtttcatcat caaatgcagt
    12601 tttgccttta tttttataat cttttaccca gttttgaact tgtgttttgc ttcttatatt
    12661 cagcatctca gcaattttgt atgatgagta tccttctttt atatataagt ttacagcttt
    12721 tagcttaagt tctttgctat attttttgtg ttttttagac atagaaaaac ccccttagag
    12781 tatacgcttt ttagacctat tataatagcc ttttatttaa atgtctacta taaggggatt
    12841 atactaaaat agagataagt ctctttttca ttgtttaaat gattaaagta tatttatatt
    12901 ataaatttct attatctttt aacataacat catgagctcc accttcaact atactagtag
    12961 aagatacaag tgtaagttta gcattttttt gaagttcagg aatgctaagt gctccacagt
    13021 tacacatagt agaacgaact ttacttaaag aaagagttac attgtctttc aaagaaccag
    13081 cataaggaac ataagagtca acaccttctt caaaagaaag tttcttatct ccaccaaggt
    13141 catatctttg ccagtttcta gcacgagcag aaccttctcc ccaatactct ttcatataac
    13201 taccatttat attaactttg ttagttggac tctcatcaaa acgagagaag tatctaccaa
    13261 gcattataaa atctgcaccc atagctaaag ctaaagttat atgatggtca taaacgatac
    13321 caccatcaga acaaattgga atataaatac ctgttttttc aaagtattcg tccctagctt
    13381 gagcaacttc aattatagaa gtagcttgtc cacgtcctat acctttttgt tcacgagtta
    13441 tacatataga accaccacca ataccaactt taacgaagtc agcaccagct tcagctaggt
    13501 aaaggaatcc ctcacggtca actacatttc cagctcctac cttaactgta tcaccatatt
    13561 tttcacgaat gaaatcaagt gttatttttt gccactcaga aaaaccttct gaagaatcta
    13621 tacataaaac atcagcacca gcttcaacta aagcaggaac acgttcagca aagtctctgg
    13681 tattaatacc agctccaact acatatctct tagaagaatc taaaagttca agtggatttt
    13741 ctttatgaga agagtaatct ttacggaata ccatatacat aagattacca ttgtcatcaa
    13801 gtacaggaag agaattaagt ttgtggtccc atataatgtt gtttgcttcc tttaaagtaa
    13861 catctttgtt agcataaact atagatgaca taggagtcat aaattcacta actttagtat
    13921 ctaaatccat acgacttata cgataatcac gacttgcaac aatacctaat aatttaccag
    13981 ttgaagttcc atcttcagta acagctactg tagaatgtcc agttttttct tttaagtcaa
    14041 gtatgtcttt taatgtgtta tcaggtttaa tatttgaatc acttacaaca aaaccagctt
    14101 tgtgggattt aacacgtgct accatagcag cttcatcttc aatagtttga gaaccgtata
    14161 taaatgaaat accaccttct ttagcaagtg caatagccat tttgtcatca gaaacagatt
    14221 gcataatagc agaagtcaaa ggtatattca tataaatatc tgcttcctcg ccctttttaa
    14281 atttagtaac aggtgttttt aaacttacat tagcaggtat acaatcagta gaagagaaac
    14341 ctggtacaag aaggtattca ctgaaagtat gtgatggtgt atcaaaataa aacgccattt
    14401 tttaatcact ccttatattt ttatactttt tataattgat agtattaatt ataacttatt
    14461 ctttaatttt attaaagtat tttgtcaaat attgagattt ttgaaaaatt taaactattt
    14521 taattgaatt tgtttaatat atattgcctt atagggtaaa gttgtatatt aaataatgtt
    14581 tagtgtttaa tagtagctaa tttgaaatta atctttagtt attcatataa ttacatttgt
    14641 atagtttata ttgataatat tgctagagag tgcataaacc tcaaaaatac agataaatta
    14701 aatctatatt agtatatcca aaagtacatt taataaattt atttaactga attattattt
    14761 tcatttctat taaatttaaa tattttataa aaatagtatt atttacaaaa tcatatactc
    14821 caaaatacta cataaataaa attaaacaaa aactaaaatg acaaatgtca ttatctaaag
    14881 tgacatagaa acggtttctt aaagttgtta ctataaaata taaactctat aataaaagta
    14941 taacaaaagg gaatcatatc gatatagaat ataattcaag aggaggtaaa attatgagta
    15001 gttctaatca atcgacaata aagacatcta tacagcgagt agggaaattt ttaagtggta
    15061 tggtaatgcc aaatatagga gcttttatag catggggtct tataacagca ttatttatac
    15121 caacaggatg gatgcctaat gaaaaattga gtactatagg cgacccaatg ataaaatacc
    15181 ttttaccact attgatagct tatactggtg gtaaagcaat agcaggacaa agaggtggcg
    15241 taataggtgc agcagcagct atgggagtaa ttgttggggc agatatacca atgtttatcg
    15301 gagcaatgat aatgggacca tttgcaggat gggtaattaa gaagttcgat aaatttgtag
    15361 atggaaagat accaacagga tttgaaatgt tagtaaataa cttttcaata ggtataatag
    15421 gtatgcttct tgcaatacta ggattttatg caattggacc agcaatagta gcagggacag
    15481 ctcttataga atctggagtt caatttatag ttagcaaaag tttattgcct ttagtttcag
    15541 tatttataga acctggaaaa gtattattct taaacaatgc aataaatcat ggtatattag
    15601 ggcctatagg tatagctgag gcaaaagaag caggtaaatc tataatgttc ttactagaaa
    15661 gtaaccctgg accaggttta ggagttctat tagcatattg gatgtttagt agaggaagtg
    15721 taaaacaatc agcaccagga gctgtaataa tacatttctt tggtggtatc catgaaattt
    15781 acttcccata tattttaatg aatccagttt taatattggc tacaatggca ggtggagcag
    15841 caggaatatt gactttttca atattaggag ctgggcttgt agcagcacca tcaccaggaa
    15901 gtatatttgc acttatggca ctagctccaa agggaggatt acttccagta ttagcagggg
    15961 ttgcagtggc aacagttgta tcattcttag tggcagcacc atttgttaag agagcttcag
    16021 caaatgaaag tgaagaagac agtacttctt tagaagaagc taaagcaaaa atgagtgata
    16081 tgaaatctgc aagtaagaac tctgagaaaa atattgagaa aaaacagctt gaagtaaatg
    16141 agattaagaa gattgtattt gcatgtgatg caggaatggg ttcgagtgca atgggagctt
    16201 ctagatttaa aaatagaata aaaaacttag atttagatat agaaattaca aattcatctg
    16261 ttgataatct tccagatgat acacaaatag tagtaactca caatacatta gttgaaagag
    16321 ttgctaagaa caattcaagt gttgagatag tttcaataaa caatttttta aatgacccaa
    16381 atttagatac attatttaaa agattagaat ctaaataaaa ataaaaagta ccactaatta
    16441 ggagtggata atttgaaaaa aaagaagatt acatctaggc aaaaaaagat aatactaatg
    16501 atagtagaaa attcaaagaa aaacattcca ataactattt ctgagattgc agggacgtta
    16561 gagttaagtt caagaactgt acttagagat atgtctggaa ttgaaaaatg gtttgatgaa
    16621 aatgatttta actttgtaaa aaaaccagga gtaggattga ttttagagga aaatatagaa
    16681 aatcaaaact ttataattga gttgttagaa gaagaaaaaa tagaaaaaga atacagcaag
    16741 gaagaaagaa atttaattat attatcaaag ctactagttt ctaacgagcc agtaaaatct
    16801 tattattttg caaaaatatt aaaggtatct gaaggggtac tgaataatga tttttcatta
    16861 gcaagtaaat ggttggaaag atttgatatt gagcttgtta gaaaaccagg gcttggtgtc
    16921 tacctaaaag gtcaagaaaa aagctttaga gaggcatatg taaatcttat atacgattca
    16981 ttcaatgaaa aagaaatact agatatggtc agaaatataa gtgaaaatat acaaacagat
    17041 aaagcaatag aaattttaag tgaaaataga cttttaaatt taatggatag gtgcataata
    17101 ataaaagttg aagctacatt aacaaagaag ctttcagatt tagatgtaaa tttagctgat
    17161 agtgcatata tagggcttgt agttcatatt tcattagcct tacagaggat aaaaaatggt
    17221 gaaaatataa ctatggataa agagtttcta aaagaattat ctataacaga agagtttaaa
    17281 ttagctaaag aaatcgtaaa aggtatggaa acagatttta acatggatat accagtagat
    17341 gaggttggat atataactat gcatataaga ggtgcaaagc aaaggtcatc ttcaaatcat
    17401 aaggctttaa acttggatga tatagaaata atggaaatta caaataagat gatagatttg
    17461 gctgaagatg aatttaaaat aagtttaaaa aatgacgaga ggttatttaa agacctagca
    17521 aatcacttag gaccttctat aaatagattg aatatgggat tagaaataag aaatcctctt
    17581 ttagatgaaa taaaaagcaa gtattcatat gcctatgatg gagttgaaaa aatttctaga
    17641 attataaaag ataaactaaa tataaattca atacctgaat cagaaatagg atatattgca
    17701 atgcactttg catcagccat agaaaaaaat cttatgatga atactaatat aaatattgtt
    17761 gtggcatgtc ccactggaat aggtacttca agattcttat caactaagat tgaaaataaa
    17821 tttcctaatt taaatatact tgaaaccata tcagctataa acatagatga agagtattta
    17881 aagggaaaag atgttgattt aatagtgtct acagttgagt taaatacaag tttaaattat
    17941 atatgtgtag gtccatttat gagtttagat gatgaacaga taatcaagga aaaaataaaa
    18001 tctatagctc aaaataaatt aattaattta aatacaaaaa atgatactaa aagtaagaat
    18061 aaagtatatg aacagataac agaaagtatg aatataggaa aagatattct tcaattttta
    18121 gaggaaatta gatttgaaaa atttgaaagt aaagatttaa gtgagcttat tgaagactca
    18181 tcaaaaatat ttgcaaaatc tagtgaagat ataatgtcta taaaagaatc gttaaaagaa
    18241 agattaaaaa tctcaattcc atatatagaa gaatcaaaaa ttctattatt acattgtatg
    18301 agtgaaagaa tagatattat gaagttatca ataataaaac tggagaatcg aatcgtgcta
    18361 gattcaaatg aagaaatcga caatgtagtt tttatgttac ttcctaaaaa ttcaccaagt
    18421 tatcaaagac aaataatgag tgaaataagt ggttcattaa tagacaatat aatttttact
    18481 aataagataa ataaattctc tatagaagag atgacattag aggtaaaaga tatagttttt
    18541 aatttttata caaacagatt aaaagcattt atagatgaat aaaattaata ttaaaaagga
    18601 aggttgatac tatgagtaaa gtattgaatg agaataatat atttttaggg ttagatagtg
    18661 tttcaaagga agaagctata actttagcag gaagaaaatt agttgaaaat ggatatgtaa
    18721 aagaagaata tataccagca atgctagaaa gagaaaaagt aatgactaca tatatgggaa
    18781 tgggagttgc tattccacat ggtgtaaatg aagcaaaaaa agaaatatta tcttctggaa
    18841 tagttatact acaattccct aatggtatag attttgatgg agaaaaagca tacttattaa
    18901 taggaatagc tggtgttgga gatgaacatt tagaaatact atctaatata gcagtagttt
    18961 tagatgatga cttaacagaa agactaaaaa attcaaatga caaacaagct tttatggaag
    19021 cttttgccaa ataatcttag gagggattta aaatgaaaaa ggcaatccag tttggagcag
    19081 gaaatattgg aagaggtttt attggaggct tgttagttaa gtcgggttat catgtagtat
    19141 ttgctgatgt aaatgaggag atattaaact caataaataa agataaaaag tatactatac
    19201 acataagaga tgtagaatgt atagatgaag taattgataa tataagtgcg gtttcatcta
    19261 taaaagaaga aataatagat gagattgtac aggctgaaat aataactaca gcagttggac
    19321 ctttagtttt gactaaaata gcctctacaa ttgcaaaagg tataaaggct agaaaagaaa
    19381 agggtttaac aagtaatcta aatataatag cctgtgaaaa tgcaatatac gcaagttctt
    19441 ctttaaaaga agaggtactt aagtatttaa ataaggaaga agtagaatat ttagagatat
    19501 atgttggttt tccaaactgt tcagttgata gaatagttcc tcctggtaaa aatgaaaatc
    19561 ctcttgatgt aacagttgaa aatttttatg agtggaatgt tgaaaaacaa ggatttaaag
    19621 gggaaatacc aactatagtt ggaatgaatt tagctgacaa ccttatggct tatatagagc
    19681 gtaaattgtt tacactgaat acaggtcatg ctataacagc ttatatagga tatttaaaag
    19741 gatataagac tatagaagag agtataaaag ataaatttat ctgtgatata gtcaaaagtg
    19801 caatggtaga aagtggagaa ggattgatta aaaaatacaa ttttgactca gaagcgcact
    19861 ataaatatat tgataaaatt ttaaatagat ttaaaaatcc atatcttaat gatgatgtat
    19921 taagagttgg aagggaacca ttaagaaaac taagtgataa agatagattg attaaacctt
    19981 taatgacagc aaaatcatat ggattatcag ttgacaattt gattcttgga ataggtgcag
    20041 cgcttcatta taataatagt gaagatactc aaagtgtaga attacaagag ttgataaagt
    20101 ctaatggtgt aaaaaaagct gttgctaaaa tagctaatat aagcaatgac gaagaacttt
    20161 taaataatat cgaaaaaagt tatatattta tgaaaaattt ataaatttac ttgaaaaaaa
    20221 gtagttctga taaagtaact ataatatatt attctacaca gtttgaatta tttgttgaat
    20281 aatagagaat tataatatat aattaaaagg aataaattaa tatatttacc tgtatatgtt
    20341 tgataatatg gttcaaatgt ttctactaag ctaccattaa tagcttgact acaggagtga
    20401 agaagatata gtttatactt attttaaatt atatattaag ttaattactt tgaattttaa
    20461 gctcctgtct atgatataga caggagcttt tttagtattt taatataaaa tacttacaag
    20521 aatagatagc aaaattagaa caaatataat ttttttatta atataacttt agaaagggct
    20581 aggtggaagt tcaatgaagg ctttacaaga aaagatattg agagaaggca gtgtttcagg
    20641 aaatgatata ctaaaagttg atagtttttt aaaccatcaa attgatgtag cttttttaaa
    20701 tgaaatagga agagaattta aagagagatt taaagaagaa aaggtggata agatatttac
    20761 aatagaggct tcaggaatag ctatagcatc aattgtttca cagtattttg ataatgcacc
    20821 tgtagtattt gcaaaaaaat cagaatctaa aaatttggat aaagatgtat atgagactaa
    20881 tgtatattca tttactaagg caagagaata ttcagttaaa gtttctaaaa agtacataaa
    20941 caaaggtgaa aatatattga tagtggatga ttttttggca aatggaagag ctgctcttgg
    21001 acttaaagat ttaattgagc aagcagaagc taatttagtt ggagtaggaa ttgtcattga
    21061 aaaaggattt caagctggag gagcactttt aaaggctaat gatgtaagat tagagtcttt
    21121 ggcggtagtt gaatctatag ataatggaac tgtaaaattt aggtaattta tgatattata
    21181 tacttgtgtt tagcacatat tttaactatg taattatgga ggatttttta tgatattttt
    21241 tatagacaca gccaatattg aagaaataaa agaagcaaat gatttaggtg taatatgtgg
    21301 tgtaactact aatccatctc taatagctaa agaaggtaga gattttatag aggtagttaa
    21361 agaaataagt gaaatagtag atggaccaat aagtgcagaa gtaataagtt tagagcatga
    21421 aggtatgata gaggaagcag aaaaattatc taagatacat aaaaatatag ttataaagat
    21481 acctatgaca gcagaaggtc taaaagcggt gaaagtacta tcaagtaaag gtataaaaac
    21541 taatgtaaca cttatattct ctgctggaca agctctttta gctgctagag ctggagctac
    21601 atatgtaagt ccttttgttg gtagattgga tgatatatca caaaatggtc ttgatttaat
    21661 tgaagaaatc gttgatatat ttagtgtaaa tagtattgag gctcaaataa tagttgctag
    21721 tgttagaaat ccaatacatg ttttacaagc tgctagaatg ggagcagata tagcaacagt
    21781 tcctcttaaa gttataaacc aaatgattaa gcatcctttg acagacaaag gaatagatag
    21841 ctttatgaag gattgggaag gtgcttcttt aaaattataa ttataaacta cgctttataa
    21901 gtatattaaa gaggttatta tggaaattct aagtaataag gctaggctat tttgcctagt
    21961 cttattttgg tgtataatat attgaaacat attattgaaa tagattaatt ttttaagtga
    22021 ggaggcgaaa aatggataaa atattaagag agatacaaag agaagataaa aaaaatccat
    22081 atactgacca agaacttgct gaaatgttaa atgtagctag gtctgaagtg atatcagtga
    22141 ggaaaaaaaa taatatttta gattcgagag aaagaagaaa aaaaatatta ataaaagata
    22201 tatcaaaaat tgtaagtgaa aatcctcaaa tgtctgaaag aaaaataact gagatattaa
    22261 tagagaatgg atataatata tcgagaagtg cagtttcaaa attattaaaa gaagaaaatt
    22321 taaatcagtc aaagattggg attaaagata aagagccagc atcatcagga aagtatgtga
    22381 aggtgagtaa agttgtagaa gaagatggat ttgaagaatt gataggtata aagggaagtt
    22441 tgaaagaaaa ggtaaatcta gctaagtcag caataatgta tcctcctaat ggccttcata
    22501 ctataatata tggagaaaca ggggtaggta aaagtgaatt agctacatgt atgtataaat
    22561 atgctataaa aaacaacatc aaagaagaga acagcccatt tatagtgttt aactgtgcag
    22621 actatgcaga aaatcctaat ctattgatag ctcaactctt tggggtagta aaaggtgcat
    22681 atacaggagc tgatgcaaat agagaaggtc ttgtagaaca agctaataat ggaatattgt
    22741 ttttagatga aattcataga ttgcctgctg ctgggcaaga aatcttattt tcattaatag
    22801 atagaggaga atttagaagg ttaggagaaa gtagttctaa tagaaaggca aatgttctca
    22861 taataagtgc tacaacagaa aacccagatt ctaatttact tcaaacattt agaagacgta
    22921 taccaatggt aataaattta cctaatatga gtgaaagacc aaaatctgaa agatatgaaa
    22981 taataataaa gttttttgaa agagaagcaa agcgagtaaa caagaatttt ataataacta
    23041 aagaagttat gtgtgcactt atgaattata agtgtactgg aaatataggt cagttaaaga
    23101 gtgatataca agtaacttgt gctagagctt ttagtaaatc aatattttct agcgataagg
    23161 taattataga tttagatagt ttaagggatt atataaaaag tggatattat gattgtaatt
    23221 gtaaaaatga aaagtattct gagttttcag ttgaggatat atatatagat atcagtgaat
    23281 tggtgagtgg aaaaaataga attttatctg ataatgagat aagcgagata tataattatg
    23341 cagaaaaaga gcttaaggtt ttggaaagta agtgtttttc agaagaagag ttaaaaaatg
    23401 catttataga aaagttagat aagaagttta atgctattaa aaacaataaa aatttatctg
    23461 atagaagaag tcgaatagac tggggaggac aattaaaaga gtctacattg gagataatgg
    23521 ataaggtaat tgtatttatt aaggagcagt ttaagcatgt aaatcaagga ttgtacttgg
    23581 ctctagcaat tcacattgag catgctatag gtaggataaa agatggaaaa actataataa
    23641 atccgagttt agataaaata aaagcatcaa tgccaatgga atatgaattg tcaagatata
    23701 taatgggtat agtggaagat ttgacacaag taacatttcc agaagatgaa cttggatatt
    23761 tggcttatta tataaataaa ttttgttata atgaggaaag tataaaagat aaagttaaag
    23821 tagtaatagt tacgcatggt aaagttggaa ttgaaatgtc taaagttgta aatcatattt
    23881 taggtataga atgtacacta ggtatagaaa tagcacttac agattcacca tcagagggga
    23941 tagagcatgt attagaagag cttcaaaaga tagaagctag aaaaggtatt ctagttctaa
    24001 ttgatatggg ttctttggta attcttggtg atgaggttga aaaaagactt ggaattagat
    24061 gtaaaactgt aaacagagta gatacgttat tggcaatgga agcaggaaaa cttgcaacca
    24121 ttgaaggaaa aagtttagat ggtataatag cagatttaaa gaaaaataag aattatgcca
    24181 tgataaatac aaataaattt agttacagaa aaaacgagta tggaaagaaa aatgtaatca
    24241 ttactttatg tttgagtggt gtaggtacag ctttaaattt aaaagagcat atagaaaaac
    24301 aaattaagga atatgacact ttaattgagg taaagccagt tgctttttta aataataatt
    24361 tagaagaaga gttaaatgat attgaagctg aatataatat tattgctatc tgtggaacta
    24421 tcgatataga ttataaaaac gtacctttta tttcttataa tgaggtactt ggtaaaaatg
    24481 gtattaataa agtattagag catttaaaca gtaaacaacc agttttaaat attgaaaacc
    24541 agttgaataa tttaatacat gaagatttga ttttatacaa ttttgaagga atttcaaagg
    24601 agtatataat tgatactctt gtttcaaagc ttgaagaagg tggatatgtt gactcaaagt
    24661 atatattaag tgtatataaa agagaagcaa tgggaagtgt tgtaatggcc tctaaggtag
    24721 ccgtgccaca tggacttcca gaaaacgtta taaagcctgc aatagcaatc gcaaggttga
    24781 ataagcctat agtgtgggac aataaattta tggtggattt ggtagtacta ctggcactta
    24841 aagaaaataa taagaaagaa atacgaagct tattttcaaa gattaatgac caacatactt
    24901 tagaagtttt attaaatact gaagataaaa atgaaataaa agaactttta agctaattat
    24961 tagtacagag aagaaagaca aaaaatttgc tttagtataa aatttaaaac tacttaattg
    25021 ctaagtagtt aattactcga tataaggcta gttctatgaa atttatttat atgattttat
    25081 agaattagct tttttcttgg taaataaaat atatgaattt atttatgcaa aaagtaaatt
    25141 ttattggaca attctatgag aatttttcct atatttctta taaaatatag aatatataaa
    25201 a
//
LOCUS       DBGJFU010000048        25144 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.048, whole genome shotgun sequence.
ACCESSION   DBGJFU010000048 DBGJFU010000000
VERSION     DBGJFU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.048"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(70..447)
                     /locus_tag="ACOIAS_001894"
     CDS             complement(70..447)
                     /locus_tag="ACOIAS_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2701606.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            788..1900
                     /locus_tag="ACOIAS_001895"
     CDS             788..1900
                     /locus_tag="ACOIAS_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2701607.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(2108..2584)
                     /locus_tag="ACOIAS_001896"
     CDS             complement(2108..2584)
                     /locus_tag="ACOIAS_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701608.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGDHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2783..4408)
                     /locus_tag="ACOIAS_001897"
     CDS             complement(2783..4408)
                     /locus_tag="ACOIAS_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2701609.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(4746..5765)
                     /locus_tag="ACOIAS_001898"
     CDS             complement(4746..5765)
                     /locus_tag="ACOIAS_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HGZ2701610.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(5762..6331)
                     /gene="mocA"
                     /locus_tag="ACOIAS_001899"
     CDS             complement(5762..6331)
                     /gene="mocA"
                     /locus_tag="ACOIAS_001899"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGZ2701611.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            complement(6438..7160)
                     /gene="yqeC"
                     /locus_tag="ACOIAS_001900"
     CDS             complement(6438..7160)
                     /gene="yqeC"
                     /locus_tag="ACOIAS_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGZ2701612.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLVENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            complement(7343..8620)
                     /locus_tag="ACOIAS_001901"
     CDS             complement(7343..8620)
                     /locus_tag="ACOIAS_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGZ2701613.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            complement(8759..11065)
                     /locus_tag="ACOIAS_001902"
     CDS             complement(8759..11065)
                     /locus_tag="ACOIAS_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HGZ2701614.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            complement(11058..11852)
                     /locus_tag="ACOIAS_001903"
     CDS             complement(11058..11852)
                     /locus_tag="ACOIAS_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HGZ2701615.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            complement(11959..13317)
                     /locus_tag="ACOIAS_001904"
     CDS             complement(11959..13317)
                     /locus_tag="ACOIAS_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGZ2701616.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(14665..15666)
                     /locus_tag="ACOIAS_001905"
     CDS             complement(14665..15666)
                     /locus_tag="ACOIAS_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701617.1"
                     /translation="MQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEVKQ
                     EMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEITVMKEDMSEVKQEI
                     NVLKVDMSEVKQEMTVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTV
                     MKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQ
                     NKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLLHN
                     SEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(15902..16987)
                     /locus_tag="ACOIAS_001906"
     CDS             complement(15902..16987)
                     /locus_tag="ACOIAS_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGZ2701618.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(17023..18336)
                     /locus_tag="ACOIAS_001907"
     CDS             complement(17023..18336)
                     /locus_tag="ACOIAS_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2701619.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLNENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDTKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(18337..20628)
                     /locus_tag="ACOIAS_001908"
     CDS             complement(18337..20628)
                     /locus_tag="ACOIAS_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HGZ2701620.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(20618..21412)
                     /locus_tag="ACOIAS_001909"
     CDS             complement(20618..21412)
                     /locus_tag="ACOIAS_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HGZ2701621.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(21451..21897)
                     /locus_tag="ACOIAS_001910"
     CDS             complement(21451..21897)
                     /locus_tag="ACOIAS_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HGZ2701622.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(21969..23375)
                     /locus_tag="ACOIAS_001911"
     CDS             complement(21969..23375)
                     /locus_tag="ACOIAS_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2701623.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(23393..24763)
                     /gene="hydA"
                     /locus_tag="ACOIAS_001912"
     CDS             complement(23393..24763)
                     /gene="hydA"
                     /locus_tag="ACOIAS_001912"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HGZ2701624.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEEIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(24896..>25144)
                     /locus_tag="ACOIAS_001913"
     CDS             complement(24896..>25144)
                     /locus_tag="ACOIAS_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2701625.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIKTIG
                     FGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSVYGK"
BASE COUNT         7829 a         4709 c         2620 g         9986 t
ORIGIN      
        1 ctttatattt gattcattta actaattatt gtaaaaacta tttattttat gataattaac
       61 tacaccaaat tattcatctt ttttaatatc tttaacccat ttttcaacag agttcaattc
      121 ttcttcattt accccctttt tgaacaagga tttcataaaa ctctcaatag aacttccatg
      181 catattatct aaaaaagttt ttgtttcaaa atgtaaatac tcttcttctc caataagaac
      241 tgtataatgt gtatgtctat caatttttct tgcacttaag aattgcttat ttactaatct
      301 tgataaaagt gttagtgttg tagtttgctt ccaattatat agtttttcca tagcttcaat
      361 gacatctttt gatgctactg taaaatctga actccaaata aatttcatga cttttaattc
      421 tgctggtggt atcttagtaa tagtcatatg attttacact cctctcaaac ttctacataa
      481 ttaaatacta tattaataga cttttcgagt caaaggtttt atttaaaata tctcatccaa
      541 atttattaat acaaaaaatt tatattttta ttcaaaatta aacacacaat ctattaatat
      601 aaagataaat atctattcta taagtataac agattatcaa attaaaattc taatatttga
      661 cataatatat acatttttta aataaatata tacaatgtat taaaatgtat gcaactctat
      721 ttcaaaatta ttttgtgata tatatatgtt ataataaatg tataaaattt tgattggtgg
      781 gttatatatg aatgcattta ttagaaaaag aaataaaaac tatgttgttt acctggaatt
      841 tgaagatgaa aaaacaggta aaagaaaaca aaaaaatatg ggtacttttg ataaaaaaag
      901 ggaagcaagt aaaagactaa atgaggtaaa agaaagctta tataatgatg gttttttagt
      961 accaagcgaa attacagtgt cagaatttct attagatttt ctaaataaat atagtgaaaa
     1021 tatatcttta gctacatata ataattatgt tagtatttgt aagaattaca ttaatccatc
     1081 tattggaaaa tataaaatac aagacttaca tccggttcac atacaaaact acatagataa
     1141 actatcttat aaactaaatc ctcaatcaat taagattcat ataaatatat tgaaacttgc
     1201 aataaaaaga gcttatagac tcaaattaat aaaagaaaat gttatggata gtatagaggc
     1261 tccaagatat aaaaaattta aaaatgagat ttatgataga gaacaaatga taaaattatt
     1321 agatttagca aaaaatacag aaatggaatt acctattaat ttggctattg gattaggtct
     1381 tagaatttca gaagttttag gtctaacttg ggataatatt gattttgaag aaaatacaat
     1441 aacagtaaat aagataactt cccgaattaa tggttctgtt attctcaaag agccaaagac
     1501 agaaagctct gttagaaaaa tatcttctcc caaagaatta atgtctttat taaaagaata
     1561 taaaataaaa cagaataaaa atttattaaa atcttccatt agaaataata ataatttgtt
     1621 gtttttcaat aagaaatgtg aacctattgc tgaagatgtt atgagtaaaa aatttaaaag
     1681 atttttagaa aagaatgaat tacctcatat tagattccat gatttaagac actcacatgt
     1741 tactttactt ataaattcta aagtacctat aaaggttata tccgagagag taggacactc
     1801 aaatataagt actactctaa gcgtttattc tcatgtactt aaagaaatgg ataaggaggc
     1861 ttctgacaaa atttctgaaa gtttatttaa tgctaattaa tattaattta tcttagcttt
     1921 aaattagctt agcttattga gatagattca ttaatattaa atgtatctat ctcaaattaa
     1981 taactactta aataatctgt tttattttaa tttatcgcct cagttattat acttttttac
     2041 gaaaaattat actttacaat aaaaaagata ttcccttata gaaaatatct ttttaaatac
     2101 tatattatta tatacttgct aacattggaa tatctaacat cttcttcatt acatcatcta
     2161 ctttttttgc tttatattca ttgtatccaa ttaaatatcc tttttcatat gaacttttta
     2221 aaatctcttt ttcatataaa ttatcagatt tggcttctgt gacacttaaa ttataaagcc
     2281 ctttacaccc atcttcataa cctttattat accctttttc tgcaaaatag tttttatgat
     2341 ttttttcgtt atgtactgaa gatattgtta ctccagaatt ttcttttgta ctagcataag
     2401 gacaaaaacc tttattgtgt tgatgtgctg aatgtcctcc acaataataa cgtgttaact
     2461 ctgatttatt tttattatga tgatctcctg atgaattatg ccctcctgag tgagcatatg
     2521 aaaaattccc tacactaatc atagctaaac taattactac agctactaac cttttcttaa
     2581 ccataatcta ttccccccaa aatgttatat atttttatta tatcatctac tatattatat
     2641 ttaaattctt aatatacaaa atattactct attctgctaa taaacatgtt taaacaacat
     2701 aaaaaacctc ctaatatttg attaaaaatt tacacactca tgcatagcat gtctcaaata
     2761 ttaaaaggtt ttatactaat aattatttat cttttccata cattttagct aattggtctt
     2821 gtatctcttt actttctaaa tactcatcaa agtccatttt tctatccatt attccatttg
     2881 gagttatctc tataattcta tttgctatag tagatataaa ttcatggtca tgagaagtaa
     2941 ataaaagcac tcctgggaac ttttcaagtc ctttatttac agaagttata ctttctaggt
     3001 ctaagtggtt ggtagggtca tctaatacaa gtacatttgc atttgaaagc attagcttac
     3061 taagcataca tctaaccttt tctcctccag atataacttg agcttctttt aatgcttctt
     3121 cacctgagaa taacattcta cctaaaaatc ctcttataaa actttcactt ttttcttcag
     3181 agaactgtct aagccagtca accaaagaat attctactcc gtcaaaaaac ttattatgat
     3241 tttttggaag ataatcttga gaagtagtta ctccccattt atactctcca ctgtctggtt
     3301 caagttctcc cataactatg ttgaaaagtg cagtagtagc tatttcatct cccataaata
     3361 ctactttatc atctctatcc aatctaaatg acacattatc aagaacctta accccatcta
     3421 tagttttagt taaattgtgt acttctaaaa cttcattccc aatttctctt gcaggtgtaa
     3481 atcctacata tggatatctt cttcttgatg gttgtatatc ttcaacttct aatttctcta
     3541 actgtttctt tcttgatgtt gcttgtttag cctttgaagc attagaacta aatctagcta
     3601 taaattcctt taattgagct atcttttctt cagtcttttt attttggtcc ttagccagtt
     3661 gtaaagccaa ttgacttgac tcataccaga aatcatagtt tccaacgtac atttgtattt
     3721 taccaaaatc tacgtcaact atattagtac atatttgatt taggaaatgt ctatcatgtg
     3781 atactattat aactatagat tcttctaaat ccattataaa attatttaac cagtttattg
     3841 atttaaaatc taagtggttt gtaggttcgt ccattaatag tatttcaggc ttaccaaaaa
     3901 gtgattgtgc taacaatacc ttaacctttt ccccaccaac tagttccttc atttgtttgt
     3961 aatgcatatc tttcgttatg ccaagaccca taagtatttt ctcagcatta gtttctgcat
     4021 cccatccgtc aagctctgca aactcacctt caagttcggc agcttttatc ccatcttctt
     4081 cattaaagtc ttccttcata tacaatgcat ctttttcttt cattatgttc caaagtctct
     4141 catgacctct tataacaaca tttaaaacag tttcttcttc atattcaaag tggtcctgtt
     4201 ttaaaactga cattctttct ttatcagtta tagatatact acctgtatta ggctctattt
     4261 ctcctgataa tatttttaaa aaagtagatt ttcccgcacc attagctcct attattccat
     4321 agcaattacc tttagtgaat tttaagttaa catctttaaa tagttcctta tcaccaaatc
     4381 taagtccaac acctgtaact tgtaacatta atatattttc ctttcttttt gctcataatt
     4441 tattttatca taaaactttg ataacatttt tattatatat tatcacgtaa atagtatatt
     4501 ttttatacta aatttgtgaa atatttatta aaataatact aaaaattgta aatttataaa
     4561 aattaacaat ctcttatatt atacttataa agaaatcaaa ttgcaacttt attcattctt
     4621 ttacacattg aaaataagtt attataattt ctaaataaat atgcttttct taaaacatca
     4681 ttctagctgt tttgtttcat atttaaaaag agtacttaat ttgtaaatac tcttttttaa
     4741 taactttata ttttagcaaa attacatgca ggataagtac atacatcaca gttcatacaa
     4801 aggccaccat gaccatatct agctatatca tacttatcaa gcttttcatc aattaaaacc
     4861 ctagataaaa ctatatcaaa tactgtagtt ttgtgataca ttgcgcatcc tggtataccc
     4921 ataattggaa cttctccata ataagcaagt aaaaacatag ctcctggtaa tattggtgaa
     4981 ccatatgtca ctaaatcagc tccagtttct tttatagctg ttggtgtaac atcatctggg
     5041 tctacactca tcccaccagt acaacagata agttctgctc cttgacttat aaattctttt
     5101 attgcactct ttataatttc tttatcatct gggcatatag tctgtcctat aacttcacaa
     5161 ccaaagcctt ttactttttc ttctattacg ggtccaaatt tatcaacaat tctgctataa
     5221 aacacttcat ttcctgtagt aacaattcct actttcttag gtttaaaagg tactacattt
     5281 actatttttc tattaccaac tacttttttt gcatcttcta attttttctc atctataact
     5341 aaaggtatca ctcttgttcc agctactttt aaacctttat ttactataaa attattatga
     5401 agagtagcca tcattatttc tccaacacaa ttcaaatcaa aaagtgcatc aacatctatc
     5461 tttaatagac catcacatgc tgctataaaa tctattttcc cttcttttat ttcactaaaa
     5521 tctaaatttt ctccagcagt tagttctttt aaaaacaatg cgccttcatt ttcatgaacc
     5581 attccttctg acttttccca tacatacaaa ttatctttac caagagataa taacactgga
     5641 atatcttctt cttttactat gtggcccttt ttaaattttc ttcctttaaa ttctccaggt
     5701 attatttgag ttatgtcgtg acataaaact tgtccaacag catctatagt ttttattagc
     5761 ttcataattt acccccaatt ctctcataat ccatttttgt atcgatatca aataacattt
     5821 ctttaggaac ttctacatac tttatttttg atgaattttt taaaatggtt ttaccttttt
     5881 catcttcttt aagtgccaac aattcttctt tcttactata tgggaatatg actggatttc
     5941 cacacatatc tttgtatctt ggaattacta taaaatctct atctaattta aaagtattta
     6001 taattttatc tatatataag ttatcaataa atggctggtc tcctacaaag aacatataac
     6061 catcacattt tgagctattt aaaactccta attttataga ttcactttga cctatatttg
     6121 catttttatt atctatatat tcaaaaccat atttattaca caataacaaa acctcttcat
     6181 attgacttac tactacaact tgatgaaaat caatcttgct aacttctcta aatacatgtt
     6241 ctattataga acagccttta taatttaata aaagtttatt tgctcccatt cttctggcaa
     6301 agcctgatgc cattatcact gcatttatca tatatatcat ccttggtatt tataatatat
     6361 tgatagttct atgattttta taatatctct attgatatta tctatatata taatatcata
     6421 taaactattt taatacttca atatcttaca caaaattttt gttttacact tccataaaat
     6481 atctctattt cactttcata ttctttaata ttttctacta attttatagc taaattttta
     6541 tatttttcat tttcaacttt attaataaat aatactcttt ttccagaata atttttaaat
     6601 aatccatttg gatttaatac tatatttttt aagttttcaa tacttactgt agaactacta
     6661 ttaatactac ttgtatctaa atttgctatt tttttaaata tttctactct atgtatattc
     6721 tcttcattaa tattcattcc aaatgatgtt atatctaata tccctatggt ttttgtagtt
     6781 ttatgatata ctactggttc tttagcattc caacctttaa gtttttttct ctttgagcca
     6841 tctccctcta ttaaaactaa atcaaattgg tctacaattt catccaaaat agaggaatca
     6901 atacttacta atttattttc attatttata taactgccac agactgttat tccatgacaa
     6961 atagatggaa taacagttcc atctattgta ataattatat tgtcataatc gtttggtaca
     7021 tatattttag ttgttgttgt aaggagaact ttcaatttat ctctataaaa atttgcaaaa
     7081 tagtttataa acgaagtttt tcctcctgct ccaactacag ttattatttc attcttttct
     7141 attatatttg atagttccac actttcacct cttataacca gttttctaat ctcttaatta
     7201 ttatagttag acctttttag taaaagtgat ttttgaaatt ccataaactc gaaagtaaat
     7261 agattctgtt gtaaacaaca aggcggatcc aattcgccgc taagctgtaa gcttacgcta
     7321 tcagtttatc ttttaaatat aattaagata ttcttactct ctgtggttgc tttaatattt
     7381 gaggattatt ctgtaaatta gataaattaa ctacttgtga tttttttagt atattaagac
     7441 tcgcatttac atctgcattt ataatgattc caacactgga tttgtactgt ccacgcgtta
     7501 ttctttcccc tttaaagtca tattttttaa cattgtccac attgatatca ggtagaaaat
     7561 catcagcaaa aaaatctgat ttactagtat agctttcttc ttgcctaata aaattgatat
     7621 tttttatttt acacagatat tctaattttc cttttatctc tccaaatggg attcttataa
     7681 aatttctata tatttttctc tccatattat cacttttttc taacttagca ttatatccta
     7741 caattaaatt tccaatatta ttttttacac aataatcaat tatataaaaa caggttttat
     7801 ttacatagtc tttaacttga ttatttctct tattccaaac tttaaattgt tttcttgtta
     7861 ttgcagttat attctttttt aattttaagg cttctatttt atcattctgt ttgtttgccc
     7921 actgattcac agattttagc ctttttccat ctatgataaa actatcacct aaattggtaa
     7981 cacatgtaca taaattttct aaacccaagt ctatagctag tgcatttttt tgatttgaaa
     8041 cttcctctga ttttggtatt tcataacaat actgaatttc aaagaaccta gctttatgtt
     8101 taggtattat tcttatttct ttaatttttt tctctagtat attttgtgga acttttatac
     8161 ttactttccc atatagtttt ttaaatgctg gagacatagg tacatttaaa atcccatctt
     8221 ctttgattct tatttgacct attattatgc taaaaaatcc ttctttatct aaatatttag
     8281 gtgattttat ccctctaaag tcatattttc cttctttggc taatttaatt aatccaaaaa
     8341 atgacctaaa cgaactgtct acttctttta atatttgttg cgctatatta ctattgagaa
     8401 gcttatagtt ctcattttct ttgcatacat gataattagc ttcatagtta agatgttctc
     8461 cttgattgaa ataatgctgt cttacattat atgtaccaac attatataag ttcttagcta
     8521 agaagcacat ctctcgtaga gcttgatatt cttttttagt taagtttttt aattgttgtc
     8581 tctgagttcc atagtgtata ttttttattc tgtttgacat gaactcacct cctttagaat
     8641 atactctact agacattata tacggtattt ttacaaaaca ccatatataa tgtccattta
     8701 attactttat tttacaaaat aatttaaatt tacttgcttt tatacacatt attatttatt
     8761 ataattgttt attattattt ttcataccca tataaacttt ttcaaaagtc ataggaagct
     8821 ctcttaaacg aactcctgta gcattaaata ttgcatttgc tatagctggt gctggtgtat
     8881 gtataactat ttctcctaat gatttagcac cataaggacc tcctgggtcg aaattctcag
     8941 caaaatctac caatatattt ccaacttctt tttttgtagg aactttatac tgtaggaaac
     9001 tatctgttaa taatctacca tctttatcaa atatagggtc ttcatacatt gcaaacccta
     9061 tacccatcat cacaccacct tctacttgta ttctagctaa agttggattt ataactgttc
     9121 cacaatcagg tacacatgca aaatttatga ctttatattc gcctgtattt ttatctaatt
     9181 ctatctctgc aaatcctgct ataaatggtt ttattttttt caacatgcca aaagattctg
     9241 ttgcttccaa aactactggt acatcatcac tagcaaatcc tattcctcca acagattctc
     9301 tacccaattc ctctaataga acaaacttgc tattatcgtt tttaacaaaa accctgtctt
     9361 ttcccaactc taaatcttct tttttaaatc ctagctttga ctctgcaact gaaagtaata
     9421 tatcttttaa tttatttgct gtctttattg ttgcatttcc tgtaaccgtt gtcgtacaag
     9481 aagcaaatgc tcctgtatca tagggacata agtctgaatc acctgtataa atagaaattc
     9541 tatctacagt agtatttaaa gcttccgcag caatttgagc ttgaattgta tcagcccctt
     9601 gacctaagtc agcaagacca gataatagtt tatatgtgcc atcttcatcc attcttatag
     9661 tgactgttgc catatctaca cgcggtatac cagaaccatg atttgctact gccataccta
     9721 ctgctcttac tttgttgttt cctatatctt tacatggata tttatcatcc caattaatta
     9781 atttcttgcc cttttctata cattgtctca gctcacaact taatatttcg tcttcagctg
     9841 cttttccatt ggtataccta tctattatat ttttaattct aaattcaagt ggatccattc
     9901 caatcttatg tgccaattca tcaatagcac tatctattgc aaatattccc tgtgtagcac
     9961 cataccctct aagtgctcct gctggtaacc tattagtata tactgttcta gcattaaatc
    10021 taacagcatc aatatcatta tatattggta aggtattgtg tccagattcg gaagaaacag
    10081 agacaccatt cccaccataa gcacctgtat tgtttaatga ctttagctct attgctttta
    10141 aatttccttc tttatctgca ccaactttaa catcaaaaaa catttcatgt ctactattag
    10201 aacctacaaa agtttcactt cttgtaaata caaattttgc tggtctacca gttttcatag
    10261 taacaaatgc agtaaatata tctgttgctg ctatattctt tcctccaaat ccaccaccta
    10321 ttctaggttt aattactctt atttttgaag atggtatacc caatactttt ctaagtattc
    10381 tctttacatt gtgtggtgat tgtaatgatg aaactacaac aattctatca tttatatctt
    10441 tatatgaata tgacctatga gtttccatca tgcaatgcga ttgactttgg gttttgtatg
    10501 aagattcaac cacaaaatca ctttctttaa gcttagcttc aacatctcca aattcaaaaa
    10561 attcttttga tactatatta ttttttgcat ccaatccaaa aggatatggc tctactatgt
    10621 ctttttctgg atgtataaca acttcatttc cttctgcttc tcttgcatca aacacaggct
    10681 ttaaaacctc ataatctacc tttatcattt ttaaggcttt aagtgccgtt ttctcatctt
    10741 cagcagccac taaagctact tcatctccaa taaatctaac tgtatcttca agtaaaactc
    10801 tatcataagg tgatgcttct ggttctgttt gacctggcac acaatatctg acctgtggta
    10861 catctttata cgtataaata cccacaaccc ctggaatttt ttcggctttt gatgtatcta
    10921 tattctttat cttagcatga gcatgtggac ttctaagtag ttttaatgtc aaaacatctt
    10981 tatgcataat gaggtcttca gtatatagag gttttcctgt gacaattgct gtactatcta
    11041 tctttcttat ccccttatta atcattatac attccctcct caatagcatt atacatcctt
    11101 ttaactaatg cacttgccat atcctttctg tactctttac tagctctcat attacttcca
    11161 taagtaagtt ccttactagc aacaatagat gccttatcta tatctttttc tttatttaat
    11221 atattactcg cttcaagtgc taatctagct ttttgaggtc ttgccccaat tgctattttg
    11281 taagtatcat tttcttttag cattgctgaa tttaatacag aaaaatctcc tgtgcatttt
    11341 cttattgagt caaaaacagc tattgcatct ttttttggta gtattacttc gaccaatata
    11401 tccctactta attcactatc caaaaactca cttaactcta gtattccttt attgtataat
    11461 ctcaccttcg ctccaacaac caaaagcgct ggtattaagt cagaaaagcc atactttgaa
    11521 aatacacttg caccaattct agccccttgt ctaaattgaa caccaactat attagaaaca
    11581 gcgcttgaaa gtattccact acagtaattt tttatagttt tatcaatttc taaacttcta
    11641 agtgaagtat ctgcacctat caaaatattt tttttatcct cttttatata atttagattt
    11701 atatctttta aatctatcgc tgttcctata tttttattac ttaattttat aaatccacat
    11761 ccacctaaaa taacattatt ttttttagaa attaaaattt tatatgcttc ttctaaactc
    11821 tgtggaactg aatattcctt tatagtaacc atcttatccc cccatcatta acttattaaa
    11881 acacaaaagt ttttaataaa tcttttattt atatttataa ttgtagtata tttaattcac
    11941 atatattaaa cctttacatt ataaactttt atttttatcc tcaaccttat cacttttatt
    12001 tccaggaaat tttaccatat ataaagcttg aattattatg gctaccattg ctcctgaaac
    12061 tacacttgaa tctgcaagta atgtctctat aaattttgga aatttttcaa gaacttgagg
    12121 agctaccatg aatccaatac cagaagccat agaaagacct aatatcatgc tattttcttg
    12181 attaaatcca tccatactaa caagtcttaa accagatgtt gtaagagttg aaaatataac
    12241 gagtgttgct cctccaacca cacagggagg aattgttctt attaaattag ctaataaagg
    12301 cgatattcct gccaaaaata atccaattcc tccaatagct agtacaaatt tacttacaac
    12361 ctttgtattg gaaacgattg cagaattttg tccaaacatc cccattggag ttgcagaaaa
    12421 taatgcacct attgtagaga gtactccatt tcctaaaaca gcagaagaaa gttcttcatc
    12481 tgttacttgt ctattaagcc ctcctaccgc agaaagtgta caagcaccca ttatatcagc
    12541 tatctcaact aaatatatta ttgaaaacat tacaacaact tcaagtctta tatctagccc
    12601 aaaagcaagt ggttttggaa gtgcaaccaa agtaaactcc tgaattgaag aaaaatttac
    12661 tatacccaaa attaatgata aaatgtatcc aaaaattatg ctaactaata ttgatgcatc
    12721 tttaaataat ccttttccat acttatttaa tcctaatatc ataagagata cagatattcc
    12781 aactataaaa ttaattggat tcccaaaagt ctcattgccc atacccccag ctaagttttt
    12841 tatagctatt ggaaaaagtc caagacccat acatgcaact attgttcctg atacagctgg
    12901 agtaaatata aatcgtattt ttttaagacc aaaaccaatt ataattatta ctataccagc
    12961 tattatttga gagccaaata atactggtag tccatactta cttgcaacag ataccccagc
    13021 tcctataaac atagctgtag ctcccattat aactggtatt cctcctccta atttaaaacc
    13081 tttaaacaaa ggtattggat ataattgtaa taaagttgat aatcctgcaa aaactaatgc
    13141 ggcttgaatc aataatgttg acatcttatg attaagtcca gctgcatttg caatcaatat
    13201 cgctggtaaa caactaccta ttaccattgc cattaagtgt tgtaacccta aaggtattgc
    13261 ttcctttagt ggtggtattc cgtctaattc aaattttgat gtactttttg ctttcataat
    13321 aaaaactccc gttacataaa acttattgaa ttatataaat acttaaaaat ctaatatatt
    13381 aaattaatga tgttcataaa tatcaactat actccaacat atatttaagt ttttagtctt
    13441 aaaaaacaga tgcaataaat ttaattatta acaaataaac gtattcttta attttgtata
    13501 cattatatac catgctttac aaatataaga gtattatagc tatgaatttt taatttttta
    13561 tagtaaattt aattcaattt aaatctttca atcttaatca ttataagtta taagttttca
    13621 taagtattta catgtttcaa gttttaaaca tttacctata aacgctactt tatagatgtc
    13681 acttcaaatc cagtgacctc atactttcat atgagcgtag actatttctt catcttcagc
    13741 attacctgct aagagcaacc cgtttccact atcaatcgct tatagtgtac tctctctcga
    13801 gatagtcgtt tgaccttcct atttctaggc ttggcgacca aatgtccatt tttaagtact
    13861 taggctctca ccatatacca tcctcactat tgtttctggt tttcaccaca ttcataaaag
    13921 tttctttcta tcttttattg tagttgcaag gctttaggat ttactggttt taaagttgtg
    13981 tcctacatag atttctctat gtacggggcc aaaagtacaa tttatttaac atactttgct
    14041 ttaccccctg taaataatat tatttactct ttcactgttt ttgttattaa attaattaaa
    14101 tatcaattaa tctaagcata aaataattat ttaatttaaa ctcaaaatat ttatgaatat
    14161 tttataatat aattctcata agacagaaaa acttaataag ctatgtatct cagttgtacc
    14221 aatgtactct cctttactat atttgtatct gcaacataaa atatacaagt ttctatctat
    14281 ttatagatat atatatctat aagcaaaata gtaatattat ttttaatagt aaaaatttta
    14341 ttcatattac tatttataat ttttatagag caattttagt gccaatattt gcatacattt
    14401 gtatagtata aaatttttat gttctcattg ataaaatttt acttatttca agcgttctat
    14461 tagattttat catctaaatt ttaggtttta tttttataaa ttaattgtat ttacataaaa
    14521 aaaaagacct aaaaacaaat ttttaacggt cttttctcaa ttttttattt tcatttctaa
    14581 aatttataat aaatagtaag aacactataa attcttttat ataaaattct tagtttgtgg
    14641 atgataaatt agtgaattaa aatactactt tactgatttt agtttgacaa tatcactcca
    14701 atttttacta gtggctaact ctactaaatt taagtctctt cttatttcag ctatatcctc
    14761 ttttatagaa ttagtgtcac cttttatata tgctatgtta tgttctatat tatcatgagt
    14821 agctttattt atttcagaat tatgtaataa acttcctaaa atattattct gttctatctg
    14881 tatctctttc attgatttat cttcacattc tagactatca atcttaattt caattccata
    14941 taatttttca tttgctatgg atacctcgtc cttaattcca ttcatatctt cattaatgtt
    15001 atttatttta ttttgcataa agtttacttc tgatttaact tcggaaacat cttctttcat
    15061 tacagttatt tcttgtctca cttctgacat gtcttttttc attacattta tctcttgttt
    15121 tacctctgat gtatcttctt tcattacagt catttcttgt cttacttcgg acatatcttc
    15181 tttcattaca gtcatttctt gtcttacttc cgacatatcc tctttcattg tatttatctc
    15241 ttgtcttacc tcagacatat cctctttcat tatatttatc tcttgtctta cctcggacat
    15301 atcttctttc attacagtca tttcctgttt tacttcggac atatctactt ttaatacatt
    15361 tatttcctgt tttacttcgg acatatcttc tttcattaca gttatttctt gttttacttc
    15421 cgacatatct tctttcatta cagtcatttc ttgttttact tccgacatat ctccttttaa
    15481 tacatttatt tcctgtttta cttccgacat atcttctttc attacagtca tttcttgttt
    15541 tacttccgac atatcttctt ttaacacatt tatttcttgt tttacttccg acatatcatt
    15601 ttttatgcta actacatctt catttatagt atataataac tctaaaatct tctcttgcac
    15661 ttgcatcacc atccctatta attattataa cattttacac aaatgctata caatgttaac
    15721 atatatatca gaattattaa aattttaatt aaacaaaatt gattcacttt attacttcaa
    15781 aactaattaa tatctaaaat aactacaaat aaacaaaaat aaggtcaaca acaaaaaagg
    15841 cttatctctt tgagatagcc tttcttattt ttatttaaaa ctttgttttt tataaaataa
    15901 tctatacatt cagctctgaa catgaaattt ttcttccaag tgcttttggt ttacaaactg
    15961 atatacaaag tccacaatat ctacactctt cttcattcaa ttccatctta tcattcaatt
    16021 ttctcgcttg atatgcacaa actctctcgc acatcttaca tttagtacat ttttcttcat
    16081 cataagtaaa ctcaaagcta agtctttcat taacatctcc catatgatta tgtactatac
    16141 cacaagcatt ttctatagta ttatatccta atctattaag attagatttt aaatcatcat
    16201 gtattttaga tattacttgt gcgcctttta aaattactac tgaacatata ccaacaaagt
    16261 cagcacctgc cataatcatt tctaaggcat catctccact tacacatcca cctaagccaa
    16321 ttattggtat attaacttgt ttttttattt catatattct ctgtaaagtt attggcttga
    16381 tagtttctcc acttaaatat cctattccat ttccacctaa taaaggttgc cctgtttcta
    16441 tgtctattct aaatgctggt cctacagaat cacaagcagt tattgcatct gctcctgctt
    16501 cttctaattt tttagcaaaa tcgcctatct catctatcat aggaggaagt ttaactatta
    16561 caggtatatt tactctttcc tttgcatctt taagcattgg tatcaaatct ctatagtcat
    16621 aagatactaa ttcaataaag tcagctcctg catttgcaac tttctctact aattcgctac
    16681 tttcttcaat tgtatgacct actgaagcta tacagacagt cccatctctt ttcatttgtg
    16741 gtatttcaaa atctatccat tgctctggct caaaatctgt ccatttttca ttatttaaaa
    16801 gagcattagc tgtatttcta accatatgtg gtgctggatt tatagctctt tcttttctta
    16861 tagtcttagt cacaactgcc cctgctcctg catcacttaa tatcttgcat ccagctgcac
    16921 tatatgttaa tggtcctgaa cctattataa gtggtgattt taattcttta cctaaaaaat
    16981 tatacattat ctgcttcccc ttttcatata tatttacatt cgttaaaatt tccaaaattc
    17041 acaagcaact tctttcattt ctttaacaat agctttttta tctaaatcaa ctaactgttt
    17101 ttcttttaaa atatgtctac catctatgta tacatttgat ataaattctt ttttaccctg
    17161 aactactatc tggtcaaata tattatcaag agtcacagga gttttaaatt catcttccat
    17221 aactattata tcagccttat taccaactgc tattttacca gaattttgaa gtcctaatac
    17281 ataagcacca tgctctgttg ccattctaaa tactaaattt gctggcatta ctgatgcatc
    17341 ttcttcaact gatttatgca ttaaaaatgc acctctcata acttcaaaaa aatcatttat
    17401 atatccgtca gtacccagtg ccacttttat acctttttta atcatttttg gtactggaga
    17461 aaaacctcca cctacttcac agttgctaag aggcatatga accactttta cacctttttc
    17521 ttttaatata tctatttctt catcatttac ttttacacac tgagatgcta atacagtttc
    17581 atccaatata tctaaatcat tatagtagtc aactgctttt ttgccaaaat gtttttcagc
    17641 atagtttggc tcatatatac tctcacataa atgaaattgc attggtgcat ttaatttttt
    17701 tgtatcttct ttagctttct taataaatct atcagaacat gtgaagcttg tatgtgtaca
    17761 cattattcca tttattaatt tactattttc tctactccac tttattaaat tagaattttc
    17821 atttaaacat ctaagtccat tttcaaagct tattctttca caactttcta aggataaaat
    17881 cgctttcatg cctatttctt ctagtatttt tccttgctca attaaagttc cttcttctgt
    17941 atttggagct tctagggtat cacaaaaagc taccacacca gattctataa gttctattgc
    18001 tgatgcttta gttgttgcct tcacttcttt caatccaatt ctattttcta taaatggcca
    18061 ccaatagtca tttaagaaac cttcaaagtc ggtaaaatgt acatttgccg gtattcccct
    18121 agaaagaact ccatattgat gcatatgacc attgacgaat cctggcatta ttatagcatc
    18181 tgatttatct attatttctt caatattttt atattttatt tcaacttctt catttggcaa
    18241 tatatctttt ataatgtcat tttctactac aacagcagaa tttgtatata tttcatcagc
    18301 agaagatatt aagtatttac ttttaatagc aatcatttat ttacctccct gatttaattt
    18361 tttcatggcc atataaactt tctcagaagt tataggaagt tctcttatac gaactcctgt
    18421 ggcattgtat attgcatttg ctattgctgg tgcaggtgta tgaataacta tctctcctaa
    18481 agatttagct ccatatggac ctgttggctc ataattatct acaaaatcaa caattatgtt
    18541 tcctatatct ttttttgttg gaactttgta ttgtaataga ttagaagttt gaagctttcc
    18601 atcagaacca tgtctagggt cttcatacat tgcaagccct ataccttgag ttacaccacc
    18661 ttcaacttgt attctagcaa gtgcaggatt cataactgta ccacaatctg tagaacaagc
    18721 ataatttatt actttaaact cccctgttgt tttatctaac tctatttcag caaatcctgc
    18781 taagaatggt ttagcactct cttgtatacc ataagattca gatgaatata aaacttcttg
    18841 atttccacca ccaacagatt cttttcctaa ttcacttaac aaaacaaact tctctacatc
    18901 ttcactataa caaactctgt cttcatatag tattaaatca tctattggta aatcgagttt
    18961 cttctgtgct acttttaata tcttttgttt taaactttca gcagatttta ttgtagcatt
    19021 tccagttaca taagtagtac aagaagcgta tgcaccagta tcatatggac acatatctgt
    19081 atctcctgta taaacggaaa ttctatcaat agatgtattc aatgcttttg cagctatttg
    19141 tgcaagtatt gtgtcagaac cagttcctaa gtctgaagac ccagaaaaca acttataagt
    19201 tccatcttca tccatcctca tatttactat tgccatgtct atgttggcta taccagaacc
    19261 atgagttccc acagacattc caacagccct caccttattg tttccaatat ccctaacagg
    19321 gtacttttca tcccatccaa taagtttttt acctctaatt atacattcat caagtgcaca
    19381 acttcttatt ggaaaattca ttattccgcc ttcagtttgt ttttttataa tattctttaa
    19441 ctttatttct gttgggtcca tattaagttt atgcgctaat tcatcaattg cacaatcaag
    19501 tgcaaatgta ccttgtgttg ctccatatcc tcttaatgct cctcctggaa ctaaattagt
    19561 atatacagtt ctcccatcaa attttattgc aggtacatta ttatatgttg gaagtacatt
    19621 atgacctgat tcagagcaaa ctgaaggtcc attatcgcca taagcgcctg tattgttcaa
    19681 tgctttcata tctatagctt ttatgtttcc ttctttatct gacccaattt ttatatcaaa
    19741 aaacatctga tgtctagtat tagtcattgc aaaagtttct tctctagtat aaattagttt
    19801 tgctggtctt tttgtcatcc aagttacaaa tgatgcatat atctctgtta cagctatgtt
    19861 ttttccacca aaacctccac ctattcttgg ttttattact ctcacttttg aaagaggtat
    19921 tcccagtgct ttagaaacct gtcttctcat atgataagct gattggtttg cagaagttac
    19981 tactaatctg ccattcgtat ctatatacgt ataagctcta tgagtttcca tcatacaatg
    20041 ggcttgtgat tgagtcatat aagaatgttc tactattaca tcactttctt taaactcttt
    20101 ttctacatcc cctttaccca ttttttctct cgatacaata ttttttgtag catccaatcc
    20161 aaaatcaaat ggacaaaaca aatcatcttc ttcatggatt aaaattttat ttccttccga
    20221 ttctcttgca tccaatatag gagtcattac ttcatattca acttttataa gtttcatagc
    20281 tttaatagca gccctttcat cttcagcggc tattatagca acttcatctc ctacatatct
    20341 aactacgtct tcaagtatta attggtcatg aggtgatgct tctggatatg actctccaac
    20401 cattgaatat ctataatttg gaacatcttt gtaagtgaat atgtttacta caccttttac
    20461 ctttaatgca ttttttatat ctatgttttt tatcttcgca aaagcatgtg gacttctaag
    20521 aagcttaatt gttaaaacat ctttatgaaa aattaaatct tctgtatata ttggttttcc
    20581 agtaacaatg gcttcactat ctatttttct tacctcttta tttaccatca cactcacctc
    20641 caatactatt atacattctc actactaagg cttttgccat atcttttctg tattctttac
    20701 ttcctcttat attacttcca aatgaaagtt cttcacttga tacttcacca gctttttcta
    20761 tatcattttc taaacttaat atttcacttg ctttataggc aatttttgct cttcttggtc
    20821 tagcgcctat cgcaatttta taaatacttc cttcttttaa cattgctcca tttaatactg
    20881 caaaatcacc tgtacatttt cttatagaat caaatacacc tatagcatct tttttaggta
    20941 atataacttc tattaatata tctttttcaa gctcattttc taaaaaatca gctaagttca
    21001 taaccccttt tttatataac ttaacctttg catctaccac tagtaatgaa ggtattaaat
    21061 ctgaaaatcc atacttagca aatacgctag ctcctactct agctccactt ctaaattgaa
    21121 ctcctactat attagaaaca gcgtttgaaa ttaccccatt acagtaattt ttaataacct
    21181 tatttaattc taaagttctt aaacttgtat ctgctccaat taatatgcta tcatcagttt
    21241 catttatata atctaaagct aagtctttta aatctattgc tgaaccaata tttttactac
    21301 ctagttttaa aaatccacat cctccaagta ttatgttgtt ttttcttgat attagcagtt
    21361 cataagcttc atctaaactt tttggaacta catattcctt tatagttatc atactctctc
    21421 cccatatcaa cttaattttc acttaaaagt ctaagtgttt aaatatttat gtattgctct
    21481 taattgacct ttatatcctg tacatctaca aaggttacca tttacataat ctataacttc
    21541 ttcttctgtt ggattttgaa gctctctttt cattgccaag accatcatta taaatccagg
    21601 agcacaaaat ccacactggt cagccccttc gtctgcaaga aaatctccaa acttctttgc
    21661 ttcatccaag acaccctcaa gtgttgtaat atgctgacct tctactctag cagtaagata
    21721 attacatgaa agcacggcct tgtcatttac catcactgta cataacccac atgagcctgt
    21781 atcacaccct tttttaacac ttacatatcc attttctctt aatgtatctg ctaaaaaatc
    21841 atctggtctt atacttaatt ctttttcttt attatttatg gtacatttga taatcaaata
    21901 aatcacctca aaactcatat ttttaagcta aagttttact atactttttt atgaataaat
    21961 tacatttatt aataaaggct attccattta tgaccttcca ttctattttt tatagttcct
    22021 cttacacaaa gtctctcagc tgtatcttga gcatctttta atgctttctc ttcattagct
    22081 gtcaatattt ttgaatcttc cattattata tttccatcta ctataacact ctctatattt
    22141 ttcatactag aagaatatac taatgtagat accggattat gcataggtat cgcttttggt
    22201 gaaagcattg gattaaatat taataagtct gcttttttac ctatctccaa tgaacctatc
    22261 tcatcttcca ttccaatagc tcttgctcca tctatagttg ccaactcaag aactttttct
    22321 gctgacattg ctaatgggtc acacttatta actttatgtt gaagtgctgt taatttcata
    22381 agttcaagca tatcttgaga gttattacta gcggctccat ctactcctaa actaactgtt
    22441 attccttttt taagcatttc tggcactggt gcaacacctg aagataagta catattactt
    22501 gctgtattat gagacacttt catatcgtat tttttagtaa gttccatatc tttttcagtt
    22561 agatacacac aatgtaccat aagcacattt ggtccaagta ttcctaattt ttctaaaaca
    22621 tctatgtcat attgcccatg caattctttt gctgcttctc tatcaaaagg agtctctgat
    22681 atatgtactg taaataaagc atcatcatat tcttttacta ctctccatag catttcaagc
    22741 atttcttgag aatttgacca tattgctgca ggagctactc ctattttgat tcttccattt
    22801 tctgtattat ggtgtttttc aaacaatctt cttacatctt tttcaaccgt ttctacatct
    22861 tgcattattc caggatgaac tccaaattga gctcctgtat tcatacatcc tcttccaagt
    22921 attcctctta ttcctaattc tttataagca tctattattc catcacaaag acctggctta
    22981 ctatgaggat acatgtagtc aaccattgtt gttataccac ttctaagtcc ttcaatacat
    23041 ccaagcattg cagcatcata agtatctttt ggctctaaat agttagctgc tggaaatgtc
    23101 atagtttcta accagtcttt aagtaccata tcatctccca atccttttaa taaagtttgg
    23161 aataaatgat tatgtgtatt tataaaacct gggaataaaa ctttaccttt agcatctata
    23221 atttttttaa catcagtata tttactttct atctctttag aatttccaat atcagcaatc
    23281 ttgttatctt ttactactaa tgccccatca aaaataactt ctctctcttt attcacagtt
    23341 actattatgg cattcttaat aagtatatcc atcattctta tttccccccg cattatttag
    23401 taatttttct tttaatatat tgacctgttg gatgttcttt aacattacaa ctttgtacta
    23461 catgatttcc tctcaaaaat acatcttcaa ctttacattt aaccttaaat ccttcatatg
    23521 gagtataatc tacattttgt ttttgagttt tataactaat tgtttcttct ttattgatat
    23581 ttaaaatgac tatatctgca tcacttccta cagctatctc acctttttta ggatacattc
    23641 caaatatttt tgcagcattc gtagaagtaa cttccacaaa cttatttgca cttattctat
    23701 tttctaagac tccatatgta tataaaagtg ctaacctatg ttcaacacca ggtgctccat
    23761 ttggtatctt actaaaatca tcaattccca agtctttttg ccccttaaaa ttaaatgaac
    23821 agtgatctgt gccaactgtt tgaatatctc cactagacaa tccttcccaa aggtaattta
    23881 tgtcttcttt ttttcttaaa ggaggagaca ttacatactt agcaccctca aatttatcct
    23941 tattatataa attgtcttct aataatagat actgtgggca tgtttctact atcatatcta
    24001 tatttttttc tctagccatt tttacagttt caagtgactc cctacaactt aagtggacta
    24061 cataggattt tgaattagat aatttagtta tatcagcaag tcttgaaaca gactctttct
    24121 caactatgtt aggtcttgta agtggatgat attttggctc caaattacca ttttgtatat
    24181 tttcttctat caatacatct agtaaatccc cattttcaca atgaaatcca actatacaac
    24241 ctaaatccct tgctttcttt aaaaccctgt atattgttcc atcatctact ttcatgccat
    24301 catatgccat atacatctta aaagatacta tcccatccga aataagtttt tccatatgat
    24361 taaatgtatc tttatttaaa catgtaattg tcatatgaaa accataatca caataacaat
    24421 ttccacttgc ttttttatga tatgcttcta caccttgcag taggttttcc ccctcattgg
    24481 cttctgcaaa atctagtatt gtagtagttc ctccagctat agctgctctt gttcctgttt
    24541 caaaattatc tgcagttgta atagaacctg cattcatatc aaaatgagta tgtgtgtcta
    24601 ttccaccagg aattacaatt ttacctgtag catcaataac cttatcacca tttatttcta
    24661 agttatttcc tatttcaact atctcttcat cttctatcct taaatcactt atataagtct
    24721 tatcagaggt aattatagtt ccacctctta gtattgttcc catatatacc tcctaacttt
    24781 taagctttaa agtattttct aagtattgta aaactttatt taaaattgta ttttctaaag
    24841 gtgctatatt cagcacctct agactatatt taaataattt attgtgaact aaatattatt
    24901 ttccgtatac agattttaat attccatagt aaccttctgt tcctataaat aactgttctt
    24961 gctctatata ctcatctatt gtgtgagcaa gattttcctt agaaggacca aatcctatag
    25021 tttttattcc agcttcacct gcatagtgac ttccattcgt acagaatgag tattgagtta
    25081 tttctgaatc tattccagct tcttttaatc ccttatatgc tgcttgaaca aattcatctt
    25141 cttc
//
LOCUS       DBGJFU010000049        24966 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.049, whole genome shotgun sequence.
ACCESSION   DBGJFU010000049 DBGJFU010000000
VERSION     DBGJFU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.049"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            160..1311
                     /locus_tag="ACOIAS_001914"
     CDS             160..1311
                     /locus_tag="ACOIAS_001914"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2701626.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1429..2067
                     /locus_tag="ACOIAS_001915"
     CDS             1429..2067
                     /locus_tag="ACOIAS_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HGZ2701627.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2068..3090
                     /locus_tag="ACOIAS_001916"
     CDS             2068..3090
                     /locus_tag="ACOIAS_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HGZ2701628.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3080..3700
                     /locus_tag="ACOIAS_001917"
     CDS             3080..3700
                     /locus_tag="ACOIAS_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701629.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3824..4510)
                     /locus_tag="ACOIAS_001918"
     CDS             complement(3824..4510)
                     /locus_tag="ACOIAS_001918"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HGZ2701630.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4524..5069)
                     /locus_tag="ACOIAS_001919"
     CDS             complement(4524..5069)
                     /locus_tag="ACOIAS_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2701631.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5297..6544
                     /locus_tag="ACOIAS_001920"
     CDS             5297..6544
                     /locus_tag="ACOIAS_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HGZ2701632.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6631..8625
                     /locus_tag="ACOIAS_001921"
     CDS             6631..8625
                     /locus_tag="ACOIAS_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2701633.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8625..9947
                     /locus_tag="ACOIAS_001922"
     CDS             8625..9947
                     /locus_tag="ACOIAS_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HGZ2701634.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10226..11512
                     /gene="grdG"
                     /locus_tag="ACOIAS_001923"
     CDS             10226..11512
                     /gene="grdG"
                     /locus_tag="ACOIAS_001923"
                     /EC_number="1.21.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     alpha"
                     /protein_id="HGZ2701635.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11598..12905
                     /gene="grdF"
                     /locus_tag="ACOIAS_001924"
     CDS             11598..12905
                     /gene="grdF"
                     /locus_tag="ACOIAS_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082007859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12645..12647,aa:Sec)
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     beta"
                     /protein_id="HGZ2701636.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13198..13914
                     /locus_tag="ACOIAS_001925"
     CDS             13198..13914
                     /locus_tag="ACOIAS_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2701637.1"
                     /translation="MYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIELFNKVE
                     DLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAFEVDAI
                     NYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVIKRKIY
                     VHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGDRIPVS
                     RLRQQIFSKVMLEFMKNRRI"
     gene            13916..15274
                     /locus_tag="ACOIAS_001926"
     CDS             13916..15274
                     /locus_tag="ACOIAS_001926"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2701638.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            15364..15798
                     /locus_tag="ACOIAS_001927"
     CDS             15364..15798
                     /locus_tag="ACOIAS_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701639.1"
                     /translation="MKVGNINGQAPITNGLDSCQSEVQGLEQKKKSLQDEIEKVRSDK
                     SIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEENNVSEVNNNLLERKKEPS
                     NNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRPI"
     gene            complement(16061..16297)
                     /locus_tag="ACOIAS_001928"
     CDS             complement(16061..16297)
                     /locus_tag="ACOIAS_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701640.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(16452..16676)
                     /locus_tag="ACOIAS_001929"
     CDS             complement(16452..16676)
                     /locus_tag="ACOIAS_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2701641.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(16773..18026)
                     /locus_tag="ACOIAS_001930"
     CDS             complement(16773..18026)
                     /locus_tag="ACOIAS_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGZ2701642.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            18286..19275
                     /gene="add"
                     /locus_tag="ACOIAS_001931"
     CDS             18286..19275
                     /gene="add"
                     /locus_tag="ACOIAS_001931"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGZ2701643.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            19347..20087
                     /locus_tag="ACOIAS_001932"
     CDS             19347..20087
                     /locus_tag="ACOIAS_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HGZ2701644.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            20303..21454
                     /locus_tag="ACOIAS_001933"
     CDS             20303..21454
                     /locus_tag="ACOIAS_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGZ2701645.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            21479..22234
                     /locus_tag="ACOIAS_001934"
     CDS             21479..22234
                     /locus_tag="ACOIAS_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HGZ2701646.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            22426..24813
                     /locus_tag="ACOIAS_001935"
     CDS             22426..24813
                     /locus_tag="ACOIAS_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HGZ2701647.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         9998 a         2613 c         4227 g         8128 t
ORIGIN      
        1 aagtttaact ataaattagg tttatatttt ataaaaattt gctaaaaaaa acaattaaaa
       61 attgtaaaga aaaaagatat aattagaact gtagtaataa atgttttatt atagataata
      121 gagttttaag tatcttaaat aatatttgga ggaatatgaa tggaattaag accaagaagt
      181 gcaaaattag aagaatttga taaagtaata gaattaataa actatgtatt tagaatatca
      241 agaaatcata aaccaactat gatggaagaa tttcctcttt tactaagtaa aaataatata
      301 gaaaatatga ttataatatc agaagatgat aaagtggttt ctgatgtaaa ctatctaatt
      361 caggatgtat ctattcaagg aaacagattg aaggttgcag ctataggtgg agtatgtact
      421 catccagatt atgaaaagag aggctattca tctaagatat tggataaagt agaagaaaaa
      481 atgttttatg atggtgtaga tattgtaatt atatcaggaa caagaagtct atacagtaga
      541 agaaattgta gtttggtaaa gagtttttat aaatatacaa taaaacctga agatgtaaaa
      601 atagcatatg aaatagttga atttgatgaa actaattttg aaaaagataa tgatttagat
      661 aaaatgattg aactatataa tcaaaatagt actagattta taagaacgag agatgagttt
      721 caaaaactat tgcatgcagc aactattgcc tggggaccaa ttggttacaa gaaggtattt
      781 ataaaggaaa ataataatat aataggatat ttgataataa gaacaataaa aaaagaagat
      841 tctactgtag gagaagttgc tgaaataggg ttaaactctg taaatgttga aaatatattg
      901 aaatatgttg caaataaatt tggattagaa tctttaaatt ataaagtaca tgtgaaaaat
      961 cttaaagacc agttgaaatg taatgggaca aaatctttgg attatcaaca aggaactatg
     1021 aaaataataa attttactaa gttgtgtgat agtttaagaa gttattttag tcaatatgta
     1081 gattttgaat tactaaaata catggaattt aaacaagttg aaaataaata tattattaaa
     1141 tacaaagaag aagaacttgt aatagaaaat ttggataagc ttaataaact gttttttgaa
     1201 aaaaatgagg aacagtacaa tgaatttaaa catctaaaaa atatatatga atttgctaca
     1261 aaggcatttc ctgtagattt tccatggact gcaaatttaa attatcaata gttttagatg
     1321 atattaaata aaattgattg atacctagag ttagtccttg ttaaataaga gaaattattg
     1381 gatattgaaa aagcaatttt gtaagtaatt agaaaggatg agtattatat ggacatactt
     1441 gatgatatag cagaatgtat attaaatatg ggtgtagata atatagagaa tttagtaaaa
     1501 attgctatag ataaaaatat tgatgtggaa gatatttatg aatatggttt aaacaaaggt
     1561 atgataggag ctttagataa gtttgagaat aaggaatact atctttcaga agttatagta
     1621 tgcacagatg cattaaataa aggtattaat cttttgaagg gaactggaaa agtaaagaaa
     1681 aaatcaaagg gagtaattct gatgtctgta gtagaaggag atactcatga gataggaaaa
     1741 aatattgtaa aagtaatggt agaagccact ggatataagg ttattgactt aggtgtaaat
     1801 agaaaaagtg aagatataat agaagaagct ataaaaaaca atgtagatat aataggctta
     1861 tcttcaatga tgacaactac aatggaaaat atgaaatcgg ttatagacga acttaatatg
     1921 attgagatag ataaaagacc aaaagtaata attggtggag gacctgtaag tatggagttt
     1981 gcagaagaaa taggagcaga tggatacagt tcaaatgctc caaaagcagt taaattaata
     2041 aataaactta taggaggtga agtttaaatg ttttcagata tgactttact tcagtatata
     2101 aatagtgaag atagaggttt ttttctgcct gatatgggta caaatggatt gttcttaata
     2161 aataaaaagg catatgaagt atatgatagt gtagatttgc aattagaatt agcaaagact
     2221 atggacaagt attttgaatc agattttata tactcattct gtgatgggat tacattttgt
     2281 gaaactctgg gtttagatac attaagacca gattatgatt ttccaagtgt attgacacat
     2341 actataactg acagagataa attaagaaaa ttagatgttc cagaccctta tacgagtgga
     2401 cgtatgccac tcaatataga gagcttatca gttatatcaa aaagtattaa aaaaccacta
     2461 tatgaatcga tacaaggtcc atttacactt gcaggtcaat tagctggagc tactcaactt
     2521 ttaagatgta taataactga taaaaaattt gtagaagaat tattggaatt tacaactgaa
     2581 ttggtaagaa gatatgctgt agctgcaaat aaagcaggag caaaatatat ttcaatagca
     2641 gagccaacat cagtaaccct aagtaaagat agatttgatg aatttatagt aaaaaattta
     2701 aataaaatct acaatgaact tgattgttgg aaaggtatgc atatatgtgg agacaccaga
     2761 gaattattag ataatatgct aagttgtaat atagatgcag taagtttaga ccaaatttta
     2821 gactatgaag aaattgctcc aatgattcct agagatatag ttttgattgg taatttagac
     2881 ccaataaaac ttcttggacg ttctaagcca gataagatta gaagagagac actaaaattg
     2941 ttaaagaaga tgagaagata tgataacttt ttatgtgatt ttggctgtaa ctgcttaaat
     3001 accactccag tagaaaattt acaagcagct ataaaggcag gaagaattag ttataaagaa
     3061 ttagatagga ttgatattga tgaattataa tagtaataaa aataaatatc ttttaaagga
     3121 tataaattta gatgatattt ttatagataa gaatattgtg ctaaagtttt tggggtatgg
     3181 aaatagaaaa gctccagata caattagtaa aatcgttgaa agagaaataa gaaatataaa
     3241 tgatatttta gatatcaaga tatatataag tgaagttgat attaataaaa cacctaaaga
     3301 atgtaaaaaa gcatttgctg tactatatac aataggggat aaaattgatt ttaagatgaa
     3361 tgactatatg gaaaattgca atatgatggc agggctggca ttggataagg taggtatagt
     3421 atgtttagat tgtattaatg agaaaataaa aatgtattta aaaggaaagt atgatagtct
     3481 caaaatatcg catgaaatat atccaggaga taaagatttt gaagtagaga gacaaaagga
     3541 tatttataac tatgtaaaaa ataaatataa tagtatagaa atagaaatta atgattatca
     3601 tcaactatcc cccataaaaa gtgttgcaat gcttatttta atgggggatg aagaaaattt
     3661 agaatcaaga tgtagtaagt gtcctagaaa atgcttttag ttaattttca agactaagat
     3721 ttgttttgtg tataaattgc aataattttt ttggtcattt tatgtataaa ctaaaaaaat
     3781 tattgcaaag taatattcta tttaatttga acagacttac aaactatagt agtttcttta
     3841 attcaataag tgtatttatt tcataagttg gtatatgatt agtataattt atactgttat
     3901 tggaatttaa ccaacaagta tctattcctg aatttattcc acctaagata tcagaggtta
     3961 agctatcccc aattattaag gcagaagatt tatcatgaca attaaacttt ttgagagcgt
     4021 atttaaatat ttcaggattt ggttttgata ttttcaactc ttcagataca accattccat
     4081 caatatattc ttttattttt gaattttcaa gtcttttctt ttgaactgca gaaattccat
     4141 ttgttattat aacaatagta tacttttttt taagatattg taatatatcg attgcacctg
     4201 gaattaaaaa tgtacattca cctaaaaaat ttaagtacat tttgctaaga gtttcagaat
     4261 caacatccaa agatatttta tttgcaaaaa gttcaaatct aagtaattta agttcattca
     4321 aagttataat atttttttca aggtcgagcc aaagcttatc acttatattt ctatatgact
     4381 ctatataatt ctcaaaatta aattctaaat caaactcaga tagaagtttt ttaaaagcat
     4441 gtgattcact tttcttaaaa tcaaataaag tatcatctgc atcaaaaaat ataaatttat
     4501 atgtcttcat ggtttttact cctttacaag atgactgcaa aaattacttt actttcacta
     4561 tttgagtcat tttgccattt atgttttgtt cctaaaggaa cttttacact atctcctttg
     4621 gacaaagtaa attcatcatc atccataaaa agtttaactt ctccctccaa aacatatgca
     4681 atttcatctc cattatgagt aatcctatca acacaagaag aagtgtttgg agctaaatcc
     4741 ataattgcaa attctaaatt accactagag tctggaatta aaatctcata tataacttct
     4801 ttgcttccag gtaaaataac tttttttcta ttatcatgtc ttactatcaa gtttttttta
     4861 tctacctcag atacaaaaaa tgtaaataaa ggaacgttta gagaagaggc tatagatttt
     4921 aatgaattta cagatggatt agcaagacct cgttctattt ggctcaataa tgatggtgta
     4981 acatcagcta gttttgctaa atctcgaata cttaagtttt gtttttttcg aacttcagct
     5041 attttttccc ctaaatttaa atcattcata taaatatcct ctctttttaa tttaaaaaat
     5101 attaacttaa aataatcaag taaaatgatt atgtagtatg aaattattat aacatataga
     5161 attaggtttt gaaaaaacta tgttcaatat aattaaataa attaaaatat aattgacaaa
     5221 gtggatacag aatacttaaa atatatttaa ataaaattaa gcaaaagata gttaatttat
     5281 gataggaggg gatattatgc aaataacgac ttttggagct atatttggtt tattaatagc
     5341 aattatttta attataaaaa aatttcaagc tgtatatagt ttaatgctag gagcatttat
     5401 tggaggatta gttggaggag caaatataac acaaactgta gattttatgg caaatggagc
     5461 tatgaatata tcaccatcaa ttcttagagc tttggcatca ggtgtattag ctggaagttt
     5521 aataaaaaca ggggcagtag ataaaatatc agaacagatt gtaaaaatat ttggtgaaaa
     5581 gagggcttta ttttcgattg ccatttcaac tatggtactt gctggagtag gcgtaaattt
     5641 ggatgtatct ataataactg tagcacctat aggattatat ataggtagaa aattaaatta
     5701 ttctaaattg tcaatactat tagctatgtt gggagggggt aaagcaggta atataatatc
     5761 accaaatcca aatactatag cagtagcaga taatttttct gtcaatctct ctagtgttat
     5821 gatggcaaat ataattccag caattattgg agttgttatt acagttattt tagcaagtat
     5881 attaataaat aaaggaaata aagtacaatc atatgaaatt ttagagcaaa gagaagattt
     5941 accaagttta tttaaatcat tatgtggtcc aattatagct atatttctat tgtttttagg
     6001 aaatgtatct cctatagtta tagacccaat gatagccctt ccaataggag gaatcgtaac
     6061 tctaattgtc acaggaaact tgaacaattc aagagaatat ttagcttttg gattatctaa
     6121 gatgcaaggg gtatgcatat tattacttgg aacagggact atcgcaggaa ttatacagat
     6181 gtctgaatta caacagagta caataggtgc tttacaattt ttaaatatgc cacaattttt
     6241 attggcacca gtttcgggta tacttatgtc attagcaaca gcatcatcaa cagctggtgc
     6301 tactatagca tcatctactt ttcatgatgc tataataaat ggtgggctat caccgatttc
     6361 aggtgcctca atagtaaatg ctgggtctag tgtattcgag caattaccac atggctcact
     6421 atttcatact agtgcaggta gtataaacat ggatataggt gaaagattta agttaattcc
     6481 atatgaagca ttgataggaa tagtaatgac tataatttca acatctattc aattagtttt
     6541 ataataaata taaatattta atgaaaaaag taggattttg ttattaaata ttgaatttat
     6601 tatagtaaaa aagaagattg gagttttaaa gtgaataaaa aaaagattgt tattataggg
     6661 attatttatt catttttagt agtattttca cttacaaata tgtatgtaaa tatggagtat
     6721 aatctaaatg tatttgaata tattaagaag tcacttccat ttacagaaga agaaaaaaag
     6781 tggttagaaa aacacaagaa tttgatttac agctctgacc aaagttcacc tcctttgaga
     6841 tataaaggta aggaagatgg acagtataag ggaattattg ttgatttaat aaattctctg
     6901 tcaattcaaa ttggtagaga tttttatttt aaaccaaata attggtggaa agaatctttc
     6961 gtaaattcaa tagatgatag tataaagttt tttgacttga ttccatcaaa agaaagggca
     7021 aataaattta tatttactga cccaatatac actttgagtg caaatatatt gaaagataaa
     7081 aagtcccaag atataaatag ttacatggat ttaaagggga aaacagtagc aattccagaa
     7141 ggtgattatt ctataaattt tttaaagcaa aaaattcaag atataaacat tttgttgact
     7201 ccagatataa agactggagt caaccattta atgtctggaa aagtagacgc agttgttggt
     7261 gatgaaccag tattgcgata ctatataaat aattatggac tttcaaataa atacagtgtt
     7321 ttaagtaatc ctatatatac taaaaaagca gttcttgctg tacctaaaca atatgaagag
     7381 ctggttagta ttttaaataa aggcattttt aaacttcaga aaaatggagt gtataaagat
     7441 ttaaagaaaa aatggtattc tacgtataat gaagttgatg atatattgta tgagcgagga
     7501 attgtgccta gtatttatct ttttataggt ataattttaa tatcaattta tgttttttat
     7561 agctatacat atttgttaaa gatagaaatc aaaaggaaaa cagaacaggt aatagaaaat
     7621 aaaaaaactt tagaggcaac ttttaatagt attacagata taataatgct tgtagatgaa
     7681 aataacaata tagtagaatc gaacaaagta ctatatgact ttatgggtga aatgagttat
     7741 aagatagcag atttgattag catgataaaa ggtgttattg aaaatacctt ctctgaaaat
     7801 actaataaaa ctagtgaaat agaaatacac aataaaattt taaagataaa cacttttcct
     7861 gtagagtata aaaaaaataa tactgagtat atagtagttc ttattaaaga tataactaat
     7921 gataaaatag tagaggcgaa attacttaga gaaaacaaaa tgatatctat aggacaatta
     7981 gcttctgggg tagcacatga gataagaaat cctttgggta taataagaaa taattgctat
     8041 ctcttaaaag ataacgttac aatggaagaa gtaaatgatt gtgttaaatc aatagaaagt
     8101 aatgtagata gggcaagtaa tataataacc aacttgttaa atttcgcaag aatatcagat
     8161 gataatttag aacatataaa tataagaaat tttattgaaa atatagtgaa acttcaatat
     8221 aaaatgttac agttaaagaa tgttgaaata aaaattgact gtgaacataa tttagtatgc
     8281 tatataaatg gagaatcgtt aaaacatgta tttataaatt taatttctaa ttcaatagat
     8341 gcaatacatc aagatggaaa aattattata tactgttatg aaaaaaatta ttgtttattt
     8401 atagatttta aagataatgg tgaaggaata aaagaagatg ccctaaagga catatttaat
     8461 cccttctata caacaaaacc aattggtgag ggaactggtt taggtttata cattacttat
     8521 aatgagatta aaaagaataa tggagatata agtgttgaaa gtaaattggg ggttggaact
     8581 tgttttcata taaaaattcc attaaataaa gaggtgacta tataatgaaa atactattag
     8641 ttgatgatga gctagaatat ggagtggtaa tgaaaaaaat tctacaaaag aagggctatc
     8701 ttgtagatat agcactaagt ggtgaagaag ctataaatat aattaaaaaa gataaaaatt
     8761 atgatttggt tttgtctgat gttatgatga aaaatatgga tggagtacag ctattagata
     8821 gaattaaatc tattaataga gatatagagg ttatattagt tacaggatat ggaagtattg
     8881 aaaatgcagt tgatgctatg aaaagaggag ctttatctta ttttataaaa agtaatccca
     8941 ttgaaaattt attagaagaa gttgaaaagg taaaggcaag taaaatatct gtttctgtac
     9001 aaaaaaataa tctggaattt actttggaat ctaaaaatag agattttaat aatatcataa
     9061 aaatagcaaa aaaagctgct tgtaaagatg ttaatatatt aatcttgggg gaatctggtg
     9121 taggaaaaga tatattagct agatatatac atagcataag tccaagaaag aatgagatat
     9181 ttgtacctgt aaattgttgt tctttttcag aaaatctatt ggagtctgag ttgtttggtc
     9241 atgaaaaagg ttcttttaca ggagcagtag atagtagaaa aggtagattt gaactttcga
     9301 ataaaggaac tctatttttg gatgaaatag gagatatacc tctaaatgtt caggtaaaac
     9361 ttttgaggac acttgaggac aaatctatag agaggatagg aagtaataaa tctattaaag
     9421 ttgactttag gttaatttgt gctatgaata aagaaccaaa agtggaaata tcaaatggaa
     9481 atataagaga agattttttt tatagaataa gtacaataac aataactata ccaccgctaa
     9541 gaaagagaag agaagactta actactctta tagaattttt cttaaataag taccaaattg
     9601 aacatgataa aaagatacac tcgattgata aggaagtaaa ggatttttta ttaaattaca
     9661 attatccagg aaatataaga gaattaaaaa atataataaa tagacttgta gttttaagtg
     9721 aagaaggcaa tttatcaaaa gacaatttaa atttaatatc aaataatgta tacatagatg
     9781 ataaaataag tattagacct ttaagagaaa taagaaaaga gtttgaatgt gagtatattg
     9841 aaaaagtttt atctttgtgt ggaaataata tttcaaatac agctaaaaaa ttggagatta
     9901 gtagaagaca gttaaccaat aaaatatcag agtataatat taagtgagaa aaaaacttct
     9961 cattaagtga gaaaaaaatt tctcaaactg tattaaaaga taaacagaaa aaatctgttt
    10021 atcttttttt attataattt taaaaattaa tatttacaat aattattgct aaattcaata
    10081 ttggtgtaaa ttaaaaatga aaatttctat gacattaata catagaatat atattttaaa
    10141 taattggcat agaaaatgct ttatatatta ataaacgtaa taaatacttt taattgcgat
    10201 taaatatatt aagggggatt ttcatatgaa gctagagcta gggaatatat ttataaaaaa
    10261 tgttgaattt ggagaaaaga cagaagtaaa agatggagta ctatatatta gtagcgaaga
    10321 aatagaaaag atagtattag aagatgaaag gattgtaagt gtaaatgttg aacttgcaag
    10381 acctggcgaa tcaataagaa tagctccagt taaagatgta atagagccta gagttaaaat
    10441 tggagatgaa agcaaaatat ttcctggaat tataaataaa gtgaagactg taggaagtgg
    10501 aagaacccat gtacttttag gagcttgtgt tgtaacttgt ggaaatatag ttggatttca
    10561 agaaggcgta attgatatga gtggtcctac tgctaaatat acaccttttt caaaaacaaa
    10621 taatatatgt attgttataa aagcaaagga tggtatagat actcatgatt atgaagaagc
    10681 agctagaatt gcaggattaa aaataggtgc atatgtaggt gaggcaggaa gagaagttga
    10741 gccagatgaa gtcattgtat atgagacaaa gccattatta aaacaagtaa aagaatatcc
    10801 agacctgcca acagtagcat atgttcacat gttacaatca caaggacttt tacatgacac
    10861 ttattactat ggggtggatg ctaaacagat agtgcctaca tttatgtatc caactgaaat
    10921 aatggatgga gctataatat ctggaaactg tgtagcacca tgtgataagg ttacaacatt
    10981 ccatcattta aataatccag ttatacatga cttatataaa agacatggaa aagatttaaa
    11041 ctttatagga gttattttga ctaatgagaa cgtattctta gtcgataaag agagaagctc
    11101 tgatatggta gctaaattaa tagaattttt aggagttgat ggagttctag tgacagaaga
    11161 aggttatggg aatccagata ctgacctaat gatgaattgt agaaaatgta gtgaagttgg
    11221 agcaaatgta gttttgataa cggatgaatt tcctggaaaa gatggcaaga gtcaatctat
    11281 agcagacgct actaaggaag cagatgcagt tgtttcttgt ggtcaaggaa atctagtggt
    11341 tcatttccca gcaatggaaa aaattatagg aactttagat tatgttgaaa tgatgatagg
    11401 tgggtataaa ggatgcttaa atgaagacgg aagcatggat gcagagctac aaattataat
    11461 agcatcaact atagcaaatg gctataatca tttgacagca agatattatt aaataagtat
    11521 ttgaatataa agttctgttg atataatcat tggtctaatt gatgtaacaa ctataaagtg
    11581 atttggagga ttaagagatg agtaaaatca gagtggttca ttatataaac aatttctttg
    11641 ctggtatagg tggagaggaa aaagcagata ttccgccaga aaaaagggct ggtgtagtag
    11701 gacctggaat agcatttcag agtcaattta atgatgatgc agagataatc tcaactgtaa
    11761 tttgcggaga tacgtatttt ggtgaaaata ttgagagtgc aactcaagaa cttttaaaaa
    11821 tgataaaaga agaaagccct gatttattta ttgcaggccc agcatttaat gcaggtagat
    11881 atggagtagc ttgtgggact atttgtaagg cagttgaaga agagttaaat attcctgtaa
    11941 ttacaggcat gtataaagaa aatccaggtg tagatatgtt taaattagat ttgcatatag
    12001 tgtctacagg aaattcagca gcaagtctta gaaaggtagt tccaataatg acaaaactag
    12061 gtcttaaatt ggttagaggt gaggaaatag gacctcctga agaggaagga tatataatga
    12121 gaggaataag aaaaaatttc ttccatgaac ttagagggtc tgaaagagca attgatatgt
    12181 tggttaaaaa gatgaaaggg gaaagctttg aaactgagta tccaatgcct gaatttgata
    12241 gagttacacc tatgaaacct gttaaagatt tatctaagat aaaacttgcc ctagtaactt
    12301 ctggagggat agttccaatt gataatccag acaaaattga atcttcaagt gctactaaat
    12361 atggaattta cgatttaaca gatatggatt ctatgtcaaa taaagaattt acaactatac
    12421 atggggggta tgacagagca tatgtccttg aaaatccaaa tttagttgta ccactagatg
    12481 tagttagaga gcttgaaaaa gaaggtgtaa ttggagaatt agtagattat tttataacta
    12541 ctacaggaac aggaacgagt gttggcaatt ctaagagatt tggagaagag ttttctaaaa
    12601 aattgttaga agatgatgta gatgctgtaa ttttaacttc tacatgaggt acttgtactc
    12661 gttgtggagc aacgatggta aaagagattg agagagctgg tatagcagtt gttcatatat
    12721 gtacagtagt accaatatca cttacaatag gagctaatag aattgttcca gcagtaggga
    12781 taccatatcc acttggaaat cctatgttag gagaagagga aagtaaaaaa attagaaaga
    12841 atatagtttt aaaagcttta aatgcactaa cagaagatat agaagaacaa acagtatttg
    12901 aataataaaa atacaacaca acactgaaat aaaaaaagat atcaaaaaat aattttttaa
    12961 aattatttta cgatatcttt tttttattag gtaaattcga aatttgcatt ttttaaaatt
    13021 aaatttataa aagtgataaa ttagtttatt ataatattga tacacaaatt caaattagat
    13081 atactatgaa aagcgagatt attacatttt ggaacaaata ttcaacatat tagatcatat
    13141 taattgaaca gaaaaaatta actttatata ataattagaa taatgataag aaagaaggtg
    13201 tatatattga tttcaattgc catttgtgat gatgaatcct atatgctaaa tcatttaaag
    13261 gaaaatatag aattatacat gaagcagaag aatttattat ataatataga gctttttaat
    13321 aaagtagaag atttactatg caatcatgaa aagtttaata ttgtattttt agatatacag
    13381 atggaagaga ttaacggaat gcaggcagct agaaaattaa ggtcatatgg taatgagtgc
    13441 tttataattt tcgttacagt tttaaatgaa tttgtatttg atgcttttga agtagatgct
    13501 ataaattatc tgataaaacc tctatctaat gagaaactat ataaaacttt ggataaaata
    13561 gttacaaaac ttgatgataa tgaaagtaat tatatcacaa tacaaaaagg ttattcatat
    13621 tggaaattaa aacttgctga tattttatat tgtgaagtta taaaaagaaa gatttatgtt
    13681 catttaaaga aagagactgt agattattat gagactattg aaaatttaga gagaagttta
    13741 ccaacaaatt tttttaggtg tcatcgtagt tatataatca atttacaata tgtatatgga
    13801 tatgaaaatg gtaatgcagt tatggaaagt ggagatagaa tacctgtttc tagactacgt
    13861 caacaaatat tttcaaaagt tatgcttgag ttcatgaaaa ataggaggat ataagatgat
    13921 taatttattt tcttccgatt tatttttaaa gtacagtttt ttattttctt ttattgtata
    13981 tagaatttta atggtaatag tgcaatacta ttttctaatc agttttacaa atgttaattc
    14041 aaaattttgg tgttttattt tatattcgat aatttcaatt tttttaatca ttataaatac
    14101 atattttaat ttatataatt tattttcttc acagattcta ttttttctta tgactttttt
    14161 atttgtaaag tacataataa aacaatctac tgttttatct atagtatcaa ctttgttagt
    14221 atatttaatt gaacaattag tatatggatt tacagctcca ttaaattact tgatatttac
    14281 tagtgatata aatacagata tttataaatt tgatataaat atgggactat cttctttaat
    14341 tactattata atagctggat ttatatattg gtatagttct aaaaagttta atattaaggt
    14401 catgaacttt gataaatata tagcaatttt gatgattcca ttgttgctta taatattatt
    14461 tatgcaatca tttgaatata gttcaaatat aaatatagat acaagtcttg ggattgtaaa
    14521 gctgctatta aatacttcta taactgaaga aattcaagct tttctttttt ctataattgg
    14581 aactatatgt gtattttttt ctctatttac ctttaagaaa ctaattcaag ctctcgaaga
    14641 tgacaaggag agggcaatta tgaatcaaca gattcatgct caaaaaaact atattgaaga
    14701 agcgaaatca cgattatcac aaactatatc ctttagacat gattttaata atcatttagc
    14761 tattgtcaat ggattgttga aaaaagacca aattttaaaa gcacaagatt atttaaataa
    14821 gcttgaaaaa gtaacaagtg acctatcatt ttcttgtaat acacagaata ttgttattga
    14881 tgctcttttc aacaataaat taagtatagc aaaacaatta ggcatagagg tagattgtga
    14941 agtaattata ccccaaagca ctaacatgaa cgattttgac ctatgtatag tgtttgctaa
    15001 tgctattgac aatgcaatta aagcttgtag agttgtagat aaacaaaata ggtatttaaa
    15061 gctttccact aagttagagg gtggattttt tatgatagaa ataaaaaata gctataatcc
    15121 aataaataat tatacaagtg gctcaggtat aggtctttta aatataaaag aaattgctaa
    15181 aaaatatcaa ggggctataa gtatagataa gacatcagat tattttcaaa taaatgtatt
    15241 gctggttatt tcattacatt gcgctagtat ttgataacaa tttcattgaa aatagtattt
    15301 gtaagctata ttatgtactt gaatagagaa agtttgatat atagaaatga ggaggttact
    15361 aaaatgaaag ttggaaatat aaatggtcaa gcaccaatta caaatggttt agatagttgt
    15421 caaagtgagg tacaaggctt agaacaaaag aaaaaatcat tacaggatga aatagagaaa
    15481 gtaagaagtg ataaaagtat tgatggtgaa acagccaaat taaaaataga agctttaaaa
    15541 aaagaaatgg aagagattga aaataaaatt caagagttaa aaggaaataa agcaaaaaaa
    15601 acaaataaag aagaaaataa tgtttcagaa gtaaataata atctattaga gagaaaaaaa
    15661 gaaccttcta ataatgaaaa tgtaaaacaa ttatatgatg tttttgaaag aagcaatgta
    15721 gacgaacaat gggataatac ttatcgtgtt ctccaagatg ataaaaactt aaaagtaaaa
    15781 tttaatagac ctatatagtt tatttataaa aatagttaat attttaatta tcaccacttt
    15841 ttagaagaat acacacttta ttttaaagac ccacatattt atttacagtt agcacattta
    15901 tatgtggttt tataactatt atttacaagt ctatattgat aacttttaca tagtatataa
    15961 tgtttaaata cttattcaaa tttatctata cataaacaaa aaaagagatt ttaaaatctc
    16021 tttttagatt gttgacttat ggctttttga tttaataaaa tcaagaagcc gtttgaattt
    16081 tatctgttgg ctgataccct tctgatattg aaacaccatt tttgtttcca aaatcgttta
    16141 aaattaagaa cttattatga atgactattt cattatgaac aataagcaga taacatgaat
    16201 cttcaatcaa tgttttaaca gtagcattaa atgcttgctt atacagttct atcgtttgtt
    16261 tgaactgtcc attaaagtaa atatgagtca ataacataac tatatctaca caaattaata
    16321 taaatttttt atactgtaca ttatctaact gttgattatc tgtattacaa catgattgaa
    16381 tttaaaaagc catcaacaca tagaatgttg atggcctgaa aatagattta aaatctcttt
    16441 tttattatat attaattatt ttttaaattt atattttttg catcagattt tctaatagct
    16501 aagtcaaacc ctctaaatct aatcactata gggtcattaa gaggagctat ctttttcact
    16561 tcaacttcag tgccatctat tagtcctaag gctagaagtc tcttcataag tttttcattt
    16621 ccagctatat tgtctataat tcccttttgt ccaagtttta agttgtaaac agtcattatt
    16681 aacacctcct aatgaaaatc attatcaaac tttatattta taatatataa caaaaagagc
    16741 aaattgtcaa tatataacaa aaaataaaca tactaagtta gtatgtttat aaaaaatagt
    16801 atacatggga tactaatagc atctagcaaa aaaccactta ctaatggtac aactaaaaag
    16861 gcttttggag aatcaccata tttttgtgtc aaagaactca tatttgccag agcgttcgga
    16921 gtagctccta ttccatgtcc aatcaaacca cttattatga tggcagaatc aaaatctttt
    16981 ccaagtactc taaagcaaat aaatattgaa aataggatta taaagattac ttgaaaagtt
    17041 actattaaga gaataggagg taataatgta gataattcaa ataaatctat attcataagt
    17101 gataaagtta aaaaaattcc caatgaaagt tcttttaaaa aatctataag tttaaaattt
    17161 aattctagta tatttatctt atcatttaaa tttctaaaaa taacagccga aaacatacaa
    17221 gttacaacag agggtaatag tatattaaac gttatataga agcatctact tataatttct
    17281 cctgtattta tacaaataaa tatgagaaga acctgttcca aaaatacaga tggtgttatt
    17341 gtttcggaga agttattatt ttttatccta ttcttatttc taaaaaattt acttccaaat
    17401 ctctttagat ttctattata tctaggaatc gacaagtcta tatttgatga tggttttaat
    17461 ttatatttat ttattagaaa tttagctaca ggacatccca atataccacc cataataagt
    17521 cctattgttg cagcagataa tccaacccca actgctcctt ctataccgag attttctatt
    17581 gtaagtccaa atgcagcaga ataaccatga ccaccttcca tagatacatt tccacacata
    17641 agccctaaaa gaggttcaag ttttataatc tttgatattg taatagctaa tatattttga
    17701 caaaatgcca atactccaca caaaatccaa tatctaaata atacactacc acctttcttt
    17761 ataagattta tgcttatttc aagtcctata gttgtaaaaa agaaggttat gaaatttggc
    17821 attaaatttc cacttataga tatatctgct atattaaaat atttaaaaaa taaatttaga
    17881 aaacaaaata atagacctcc tattactggt gcaggtatac agaaattatt taaaaagtta
    17941 actttattct tcaataaatt accaattaaa aagaagaata tagatagtgt caaggtttgc
    18001 actatatcaa aacttaaggt aatcaaaata agtcctcctt tgtaattcaa agttttaatt
    18061 atataactta aattcttatg tattgaaatt ttattatcaa atgtatatta ataaatgtat
    18121 attaaaaaga atcagtagat ataattagat agtcataaat taaattaaaa tattttaatg
    18181 agatatgact tttttagaat taaaaaccgt agtatattga tataatagaa ataagactta
    18241 aaaatctgtt gatagtatta tttataagta cagtaggagg ataaaatgtt tgaaaattta
    18301 cctaagatag atttacattg ccacttagat ggaagtgtta gagtagaaac catgcttgat
    18361 atagctatta aagagaaaat agatttacct tcaaataata tggatgaaat aaaaaaatta
    18421 gcaaaggttt cttttaattg tacttcacta gatgaatatt tagaaaaatt tgatttaccc
    18481 ttaaaagtaa tgcaatctaa ggaaaattta aaaagaatta cttttgaact tttggaagat
    18541 gcaagtagag aaaatgtaaa atatattgaa attagatttg caccattact tcacacccaa
    18601 aaaggtatga gtgttaaaaa tataattgag ggtataattg aaggaattag agaagctgaa
    18661 agtatttatg atataaaagg caatttaata ttaggttgta tgagaacaat gacctcaaaa
    18721 gaggcattat tagtaattga agaagggaaa tcatttgtaa ataaaggtgt tgtagcagta
    18781 gacttgtgtg gtccagagaa agagggattt tgtaaggaat ataaagatgt ttttaaatta
    18841 gcaagagaat atggatataa agttacaata catgcaggag aagctgcaag tggagaaaat
    18901 gtattggatg ctataaatat tttaaaggcg gataggattg gtcatggggt aaaaattaaa
    18961 gaccataaaa aggcgtataa tttagttaaa gataaaaaaa tattgcttga attatgtcca
    19021 actagtaatg tacaaactaa gacagtagat tcttatgaag cacatccatt ttatactttt
    19081 tataaagata atttacatgt aagtataaat actgataata gaactgtatc agatataaat
    19141 ttaagttcag aactaaatgt tatatttgat acttttaaac taggattaga agactataaa
    19201 ataatttata gaaatgcagt agaagctagt tttgcagata aagaaactaa agagtatttg
    19261 aatagtttaa tttaatagat aaaaatattt aaactttaaa ttaagctatt aaattgataa
    19321 aaatacattg tattaggaga aaaaatgtga aagattatga agatgttgtt tatattaaac
    19381 tagtcaaaaa agaacttgaa gaatggaaga aaaatatatt aaagaaacct tctatatttg
    19441 acaaagcatc aaagggtata cagaataaat ttaatggagt attgccagat aaatatcatg
    19501 aaataattac ttcttctata aaagtaatga caaaaaccgt actgtttgga tataaatatg
    19561 ctacaaagcc accacttaga aatatgtcat tagaagaaag agaaaaatta gttttagaaa
    19621 aagctaaaac ttacaagact acagcaatga ttgaaggggc aggaacaggg gcaggaggaa
    19681 tcctcttagg tttatctgat tttcctttgc ttttaggtat taaagtgaaa tttttatatg
    19741 atgtagcttc aatatatgga tatgatgtta aagattatag agaaaggatt tatatattaa
    19801 atatattaca gttggctttt tctagtcaac agcatataaa caaaactttt aaattgatga
    19861 ataactggga tgaatatgta aatagtctac cagaagacat caatgatttt gattggagaa
    19921 catttcaaca agagtatagg gattatttag atttagctaa gtttttacaa ctaatgcctg
    19981 gaataggagc atttgtagga ttttatgtga atggaaaatt aattgataaa ttatgtgaaa
    20041 ctgctgtgtc tgcttataga ttgaggattc gagagcttaa gttataagaa aaattataac
    20101 ataaaaaatt agttatacaa tatatctata tgtatctaat tttgcattga taaaaacaat
    20161 tatggataaa aggtaaagca tgatagaatc taatgtagag gtttgtattt taatggaaaa
    20221 atttagtttt tattgcttaa gatacaaatt gaattacgaa gagattgaat tatgaagaga
    20281 agtaattggg aggaatattt tcatgacaaa tttgccaaaa caatttgata gcttcaatga
    20341 agctagacaa aaaggattta taaatgcaaa ggaacttaag gaaagtggga aaaagatggt
    20401 tggagttttc tgtacattta ctccagttga aattccaatg gcagctggag caactgtagt
    20461 aggtgtatgc ggcgttagtg aagagccaat acctgatgca gaaagggtgc ttccaagaaa
    20521 tctttgtcct ttaattaaat caagctatgg acatgcaata acagatacct gtccttattt
    20581 ctatttttct gatttgttga taggagaaac cacttgtgat ggtaagaaaa aaatgtatga
    20641 agaacttgca aaagttaagc caacatatgt aatgcatttg ccaaatactt caaaaggaga
    20701 atttgcctat aaactttgga aagatgaaat gataagatta aaagaagagg ttgagaaatc
    20761 tttaggtgta actataactg aagaagatat aagaactgct ataaaagata aaaatgaaga
    20821 aagagaactt ttaaaagagt tttatgcact tggtaaatta caaccatctg cacttacagg
    20881 ccttgagtta cataatgttt tatatcaagc aggttttaaa tttgaccgtg cagaacttaa
    20941 acattcttta agaaaagtta tagatgatat gaaagaaaga tatgaaaaag gtgaatgtcc
    21001 tgttcaaaaa gataagccaa gaatattgat aacaggttct cctattggtg gaataagcga
    21061 aaaaatagtt aaaactttag aagatgcagg ggcatcagtc gttgcatatg aactttgtgg
    21121 agctattaga tcaaacgatt tattggttga tgaagaaata gaagatgtat atgatgcttt
    21181 aacacaaaaa tatataaata taggttgttc ttgtatgatg aataatgaca atcgtattga
    21241 attattagat agaataatag atgaatataa tgtagatgct gtaatagatg tagttttaca
    21301 agcatgtcat acatttaata tagaaagtta tagaataaga gaatttgtta caaaagaaaa
    21361 aaataagcca tttatgtctc ttgaaactga ttattcaaaa tcagatactg aacaattaag
    21421 aactagattt gaagcgtttg tagagatgtt gtaggaataa ataaaagtag gaggaactat
    21481 gtttagtata ggagttgact cagggtcagt ggcaacaaag ggtgtgctat ttgatggtga
    21541 aaaaattatt aagaaaataa ttataccaac tggatggagc cctaaaagta catctaaaca
    21601 ggtctatgag ttgctaagta gtgaaattga caaaaaagat ataaaaaagg tggtaggtac
    21661 aggttatgga aggggagtaa tggattttgc tgataagaaa gtaacagaaa taacttgtca
    21721 tactagaggt atttactttt taaacaagaa cataaggact attttagatg taggtggtca
    21781 agatagtaaa gttataaatt tagatagaga tggaaatgta tttaacttta taatgaatga
    21841 taagtgtgct gcaggaactg gtcgattttt agagattact tctaaccttt taggaagcga
    21901 tattgaaagt atagatacat tagctaaagg atatgaacca gttaatatat ctagtatgtg
    21961 tacagttttt gctgagtctg aaattgtaag tcttctagct caaaacattt ctactggaga
    22021 ggtagcagca gggatactta aatctattgc aaataaatcc acctcaatgt tagcaagagg
    22081 tgaggtaata gatgaagttg catttactgg agggcttgca aaaagtaaag aactagtaaa
    22141 aatgatagaa gaaatactgg gtaaaaaaat ttttattgca gaagatactc aaataatagg
    22201 agctttagga gctgctgtaa taggatttag ataaaaacat ataaaaagaa aatgagaagc
    22261 tgaatttatt ataaataaat tcagtttttt attttatttt atttatttat aaaggaaaac
    22321 tttggtataa ttgatttaaa tgattgtata tttgtaaata cttgatattt agtcatatat
    22381 aacattataa ttttaaaaat tagctcaaaa taagggggag aaagcgtgaa aaaatttact
    22441 tcaaaaaaag taacaaggtt tgttgcgctg tttttaatat ctattttagt aatgtcaaca
    22501 attcctgtat cagctgacaa tagcaataca ttgaatcaga taaaaaaagc agaatacagt
    22561 gagacgtata aacaatatct aaatgatgct aaaaatggat gtacagaaaa atataatggg
    22621 attataccaa atccatatca gttggaaggt actcaaatcc aaagtaaagg aagaacagct
    22681 ccaacatctt atgaccctag aaaattaggt cttatgacat caataaaaaa tcaagaggat
    22741 ttaggaatct gttgggattt tgctgcaatg gcaaccttag aaagtttctt aaagttaaat
    22801 aattatggag actatgattt atcagaagaa cacttaagat ggtgggcttc agatggtgaa
    22861 tatggatggt ctgtaaatga tatgaatggt gctttaaatt atgaagctat gggatattta
    22921 acatcttggt caggtcctaa attagaaaaa gatatcccat acaatggtag agtatctaaa
    22981 gcacaaggag caaaaaaacc aactaatatg aatacagcac ctactgtttt taatgtgaca
    23041 gatgctgtgt gtgtaagcaa tgacataaat tctacaaaaa atgctatatt acagtatgga
    23101 gctgtaacat ctggttatta tgaagatatt aaatatcaaa gtgatgacca aaattcatat
    23161 tattgtccaa caaaaagttt taatacaaat catgctattt caattgtagg ttgggatgat
    23221 aactattcta aagataattt taatgcaaat attagaccat caaaaaatgg tgcttggtta
    23281 ataaaaaata gctggggaga ttataattct gagggtggat atttctggat atcttatgaa
    23341 gacaagacac ttatgtctga tatagataat ttttcaatca aaggtggtaa aaaaccaaat
    23401 gatgatgaga agatgtatca acatgactat gcaagtattg tacctttaat ttcaaaaaag
    23461 ataacagctg caaatgtatt tgatttcaat agaggcgatg aaactctaaa atctgtaatg
    23521 ttcttaactg aaagtatagg agctaagtat gaagtatatt atgctccagt agtaaatggt
    23581 atacctcaag aaaacaatat gaaaaaatta aaagaaggta ctgcccaata ttcaggatac
    23641 ataacagttc caattgattc ttttgatata ccagaaggaa agggtgcaat tgtagtcagt
    23701 atagagggga aaaatggaga gtcaactata ggttctgagt caaatgtacc aggatatgat
    23761 acatttaaag caaaagctaa tttaggtgaa agttatatca tagataatac tggaaaattc
    23821 tttgatataa atagagatag taacttctat ccgtgtaatt ttactataaa agctgttaca
    23881 gaaaagtctt ctggtgagag tatcccaaat gagtctttaa ttggtagtga tagatatgaa
    23941 actgctatca aagttagtca aaatggtttc aattcttcag aaaatgtagt tttagtaaat
    24001 ggcagttcaa tagtagatgc attggcagca actcctttta catctgctat aaattcacca
    24061 atattattga cacaaaaaga agctttaaat tcaaaaacta aagctgagat acagagatta
    24121 ggtgctaaga aagtatacct aataggtgga gaaaattcta taagtaaaga aattgaacaa
    24181 caattaaaaa gcttaaatat atcgatagag agaatatcag gaagtgatag atacaagaca
    24241 agtttattat tagctcaaaa gttaaatggt ataaaaaatg tatctcaaat agcagttgta
    24301 aatggagtga aaggattagc tgatgcaatt agtgtaggcg ctgcagcagc agaaaataac
    24361 ataccaatca tacttgcaaa tgaaaagagc gaattgcaag gagctgatga atttctaaat
    24421 tcattaaata tagagaaatc ttatattatt ggaggaacag catccctatc aaacaatcta
    24481 gaaagtaagt taaaaaatcc aactagatta tctggtagta gtagagacga aactaattca
    24541 aagataatag ataattttta taaaaaagat actttaaaaa atgcatttgt agttaaaaat
    24601 ggaatcaaga atcaaaatga tttaatagat ggattggcag ttggtccttt aggagcaaaa
    24661 actgggtcac cagtaatttt agttggtgat aaattagctg atagccaaaa agaagttctt
    24721 aaaaataaga ctttagaaaa agtaactcaa gttggtggag gagcaaataa aaatgcattt
    24781 aaagagttag taaaattaaa aagtgctaaa tagagtactt aagtattaac atatattagt
    24841 gtgtattagt aacttatctt aataaaagct gaaaataatt acttcaaatt attgataaat
    24901 aagataatca atgattgtaa agttttgttt tcagctttaa atttatgtag tataagttta
    24961 aaaaca
//
LOCUS       DBGJFU010000050        24943 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.050, whole genome shotgun sequence.
ACCESSION   DBGJFU010000050 DBGJFU010000000
VERSION     DBGJFU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.050"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            306..1190
                     /locus_tag="ACOIAS_001936"
     CDS             306..1190
                     /locus_tag="ACOIAS_001936"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2701648.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ANKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            1392..2942
                     /locus_tag="ACOIAS_001937"
     CDS             1392..2942
                     /locus_tag="ACOIAS_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2701649.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            2971..3618
                     /locus_tag="ACOIAS_001938"
     CDS             2971..3618
                     /locus_tag="ACOIAS_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HGZ2701650.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(3760..4020)
                     /locus_tag="ACOIAS_001939"
     CDS             complement(3760..4020)
                     /locus_tag="ACOIAS_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HGZ2701651.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            4237..5520
                     /gene="hflX"
                     /locus_tag="ACOIAS_001940"
     CDS             4237..5520
                     /gene="hflX"
                     /locus_tag="ACOIAS_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGZ2701652.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            5528..6205
                     /locus_tag="ACOIAS_001941"
     CDS             5528..6205
                     /locus_tag="ACOIAS_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGZ2701653.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDKAIKSLE
                     NAKNLKK"
     gene            6291..6734
                     /locus_tag="ACOIAS_001942"
     CDS             6291..6734
                     /locus_tag="ACOIAS_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGZ2701654.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            7039..7683
                     /locus_tag="ACOIAS_001943"
     CDS             7039..7683
                     /locus_tag="ACOIAS_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HGZ2701655.1"
                     /translation="MSNYRTLHEFGMDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(7769..8146)
                     /locus_tag="ACOIAS_001944"
     CDS             complement(7769..8146)
                     /locus_tag="ACOIAS_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HGZ2701656.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(8146..8532)
                     /locus_tag="ACOIAS_001945"
     CDS             complement(8146..8532)
                     /locus_tag="ACOIAS_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGZ2701657.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            8711..9466
                     /gene="nadE"
                     /locus_tag="ACOIAS_001946"
     CDS             8711..9466
                     /gene="nadE"
                     /locus_tag="ACOIAS_001946"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HGZ2701658.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            9549..10268
                     /locus_tag="ACOIAS_001947"
     CDS             9549..10268
                     /locus_tag="ACOIAS_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2701659.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(10435..11439)
                     /locus_tag="ACOIAS_001948"
     CDS             complement(10435..11439)
                     /locus_tag="ACOIAS_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGZ2701660.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            11705..12601
                     /locus_tag="ACOIAS_001949"
     CDS             11705..12601
                     /locus_tag="ACOIAS_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HGZ2701661.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREMNLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            12739..14001
                     /locus_tag="ACOIAS_001950"
     CDS             12739..14001
                     /locus_tag="ACOIAS_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGZ2701662.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            14077..15270
                     /locus_tag="ACOIAS_001951"
     CDS             14077..15270
                     /locus_tag="ACOIAS_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HGZ2701663.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSIKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            15899..16693
                     /locus_tag="ACOIAS_001952"
     CDS             15899..16693
                     /locus_tag="ACOIAS_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HGZ2701664.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGVFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            16788..18128
                     /locus_tag="ACOIAS_001953"
     CDS             16788..18128
                     /locus_tag="ACOIAS_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HGZ2701665.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            18265..19497
                     /locus_tag="ACOIAS_001954"
     CDS             18265..19497
                     /locus_tag="ACOIAS_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HGZ2701666.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            19537..20679
                     /gene="acrC"
                     /locus_tag="ACOIAS_001955"
     CDS             19537..20679
                     /gene="acrC"
                     /locus_tag="ACOIAS_001955"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0043958 - acryloyl-CoA reductase (NADH)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HGZ2701667.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            20690..21484
                     /locus_tag="ACOIAS_001956"
     CDS             20690..21484
                     /locus_tag="ACOIAS_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HGZ2701668.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            21498..22517
                     /locus_tag="ACOIAS_001957"
     CDS             21498..22517
                     /locus_tag="ACOIAS_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGZ2701669.1"
                     /translation="MRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLAD
                     QLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERKK
                     PEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMATI
                     VCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSVN
                     LTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGTT
                     VRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEMI
                     ESLNR"
     gene            22616..23992
                     /locus_tag="ACOIAS_001958"
     CDS             22616..23992
                     /locus_tag="ACOIAS_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HGZ2701670.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNRSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(24324..24668)
                     /locus_tag="ACOIAS_001959"
     CDS             complement(24324..24668)
                     /locus_tag="ACOIAS_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701671.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         9480 a         2887 c         4729 g         7847 t
ORIGIN      
        1 aattgtaaat tttgacttag ctaagttggt ttttttactt aaagtattat ttttaaaatg
       61 aaaattttta agttgcataa aacacctctt tcttattaaa ttattttaat aatagaattg
      121 aagttttctt gctgcttcta tattatgttg tgcaagaatc atgttttgat gcttggaaaa
      181 ctagctcaat aacaccttag ttttcataaa aataaataaa ataaatagtg tatatgataa
      241 aataaataca tattcatata aataaaaata aacaataact gtctagcata agaaaggagc
      301 aaaaaatgaa aaaatataga ttgttagtta cttgtataat tgccatggga atcataatta
      361 taggaacatc aaatccagtt agcaattttt acaagactac aaatttgatt agttcaggaa
      421 atatcacaaa tgaaaaagca aataaagatg aaaattcaaa caaaaataat aaagataaaa
      481 atgaagacaa atctaattct aaaagtaata aagataagtc atcagaggac aaatcgtcag
      541 ataataaaaa tgaaattcaa aaaaataaaa aacttttaat ttgtatagac ccagggcatc
      601 aaggaaaagg agatagcaat ttagaaccag tagctccagg ttcttcctct aaaaaagcaa
      661 gagtatcttc aggaacagag ggtattgcta caaaaaagcc agaatatgtt ttaaacttag
      721 aagcatctct tgttttaaag agtatattag aatctaaagg ttataatgta ataatgacta
      781 gagagacaca tgatgtaaat ataagtaatt cagaaagagc aatacttgct aatgacaaaa
      841 aagcagatat ggttgtaaga atacatgcag atagtttaaa taattcttca aaaactggag
      901 catctatatt gattcctgaa aaagatggaa aatatacagc tcctatatat gaagaaagta
      961 ataaatgtgc tgaatttatc aaacagaata tggaacaatc aggtatacaa atcaatggaa
     1021 tcgttcaaag aggtgatttg actggattca actggtcaaa agtaccagct gtattagtag
     1081 aaatgggatt tatgagtaac tataatgaag accaaatgat gtcaaatcca gactaccaaa
     1141 gaaaaatgat gcagtgtata gctgatggat tggatgcgta ttttaaataa gatatgaata
     1201 gagaaaaagt taggttggta ttattttcct aatttttatc tctatttttt attgtacata
     1261 acacaaagaa gaagtttatt ttaggttaaa ttgtcgtaaa tataactaaa aatattggga
     1321 atacttgatt taagtttaaa aagaatatat tatagtatag aatgtgtaaa atttttttga
     1381 gaggtgttga aatgctagta gtagaaaatg tgagtcatgg atttggtgcg agaacaatat
     1441 tagaaaatgt atcctttaga cttagaaaag gtgaacatat tgcactagtt ggtgctaatg
     1501 gagaaggaaa atcgagtttt ttaaatataa taacaaagaa gcttatgcca gatgctggaa
     1561 atataaaatg gtcttcaaga gctacagtag gttacttaga ccaacatacg gtattaagca
     1621 agggaaaaac tataagagaa gtgcttagag aggcttttaa gcatatgttt gacttagagc
     1681 aagaaatgat tgctatgtat gacaaaatgg gagaagctag tgatgatgaa atgagtaagc
     1741 ttcttgaaga aacagcagag atacaaacta tacttgaaaa tagtggtttt tatatgatag
     1801 atgcaaagat acaagaagtt gcaaatggtc ttggtcttgg agaaattggt cttgataaag
     1861 atgtaactga cttaagtgga gggcaaagaa caaaagtact tcttactaaa cttctacttg
     1921 aaaatccaac tattcttata ttggatgagc ctacaaacta tttagacgaa gagcatataa
     1981 catggcttac taagtacctt caagagtatg agaatagttt tgtcttagtt tctcatgata
     2041 ttgaatttat aaataataca tgtaatgtaa tatatcatat ggaaaatgga gagttaaata
     2101 gatataaagg aaattatgat gaatttgtaa gacttaatga tataaagaaa cgtcaagaag
     2161 aacaagctta tgataaacaa gttgaagaaa gaaaaagact agaggatttt gtagctagaa
     2221 ataaagctag agtagcaact agaggaatgg ctaacagtag acaaaagcaa cttgataaga
     2281 tggaaatatt agaaagacca aaagaaaaaa taaaacctac ttttgctttt aaagatgcta
     2341 gagcagctag taaaataatt tttgaaacag aaaatttagt tttagggtat gatgaagcat
     2401 tgacaaaacc acttaatttc catttagaaa gaggaaaaaa gattgcctta aaaggaatga
     2461 atggtattgg aaaatctact ctattaaaaa ctttacttgg aataataaaa ccatttgaag
     2521 gaaatgttaa attaggtgac tatttggaag ttggatattt tgaacaagag agttcaagag
     2581 aaaatagtaa tacacctatg gatgaagttt ggagtgaatt tccagggctt acaaactttg
     2641 aagttagaca ggctcttgca aaatgtggac ttacaaatga acatataacg agtcaaatga
     2701 gagtattaag tggtggagaa gctgctaagg ttagactttg caaagtaatg ttaaaaaata
     2761 taaatttctt agtattagat gagcctacaa atcatttaga tgttgaggca aaagatgaat
     2821 tgaagaaggc tatcaaagag tttaagggaa cagttctttt agtatgtcat gagccagaat
     2881 tttattcaga aattgttgat gatgtatgga atatagaaga ttttacaact aaaatcgtgt
     2941 agtgatttaa cttattgctg gggggatttc atgaaaaagt ttctaaatgt acttttttca
     3001 atttgtatta gtattgttat aatagtaggt gttattaatt ttactgttgg atttaaacaa
     3061 ctttattatt ttgacattga ttacttaaat atatcagagt tatctggttt atctaaagat
     3121 gatataaaat tgaactacga ttatctaatt gattataact taaataagaa tgtgtctgag
     3181 ttcaaattac caacattaaa atcttctcca caagggaaga tacattttga agaagtcagg
     3241 aatatatttc aaaatataaa taaattagct aaattattat tggtagtttc tttagtagga
     3301 attatattga gtgtaaaaaa taaaaatata aaaatcttaa aaactacatc aataacactt
     3361 ataattatgc cattattatt aacagttcca attttattaa attttgaaaa gtcatttatt
     3421 atttttcata aattattatt tagaaatgat tattggatat ttaatcccga tttagaccca
     3481 gttataaata tattgccaga ggaatttttt tttcactctg gaatgatgat tttaatatta
     3541 attttgttag taagtatagt actgtttgta atgtataaat tatgtaaatc tttatcaata
     3601 aaaatatttc aatgttaaat agagggatat ttttatttcc aaaataagat aataaaaaag
     3661 gattagatta aaagttatac aataaaaata gctgtgcaat aatatttaga ctatgaaagt
     3721 agttaaaaaa tatttatgca cagctattta taatagaatt tactttttac tagtgatttt
     3781 aactaaatct tgaaatttac tcataatgta attgtaatta tcttttttat gaggtaatat
     3841 acaactactg caatctttga tacctttatc tgtatactta aagtttccac cacagttttc
     3901 tcctaaagca tacaaaggac aatagcaaaa tagacagtta aactcttctg gtttattagt
     3961 tttatgacaa gggaaaaatt cgcaatcttt gtgattaaaa aacttataat tttcgcccat
     4021 attttcctcc taattaaata aaatttaaat tttttatact atataagatt ttggtgataa
     4081 tataattata ctaaataatt ttatgaatag tcgataatat agtattatta atcatagtat
     4141 taatggtata attagtcaac agaaaaataa tgtaaaaata atgtaagtgt attttgtaat
     4201 tagaatatgt tttataacaa aaaatgaggt gattaagtga aagaaattca agaaaaagca
     4261 cttcttgtag gtctgaattt gactacaata gtgaaaaaaa atgatgatat agacactaat
     4321 gaatctatgg aagaattaaa agaactaaca aaagctgctg gggcagaagt tgtaggtagc
     4381 ttaattcaaa ataaacactc tgtagatgca gcttattata taggaaaagg taaagtagaa
     4441 gaaataagag cctatagtga ctctttagat gctactttag taatttttaa tgatgaatta
     4501 tctggtgctc aaatcagaaa tatagagaat gttgttggaa gaaaagtaat agatagaact
     4561 actttaattt tagatatatt tgctcaaagg gcacttagca aagaaggtaa attacaagtt
     4621 gaattagcac aattaaagta tagattgcca cgactttatg gaatgggtgg tgagatgagt
     4681 agaacaggtg cagggattgg tactagaggt cctggagaac aaaaactaga aatagataag
     4741 agacatatac ttaataaagc agcagatata agacgtgaac ttaaagaagt aaaaaagaat
     4801 agagagacac agagagttaa gcgtttaaag tcaaatatac cgattgtagc acttgttggg
     4861 tatacaaatg caggaaaatc taccctgttg aacgaattaa taaagacaca caaggattat
     4921 gaacaagaaa aagaagtttt tgtaaaagat atgctttttg caactttaga tgtaaccttg
     4981 agaaaggctc ttttgccgaa taaaaaagaa tttttagtag tagatactgt tggatttgta
     5041 agtaaacttc cccatgattt agtagaagcg tttaaggcaa cattggaaga agtacagtat
     5101 gcagatttaa tcttacatgt aattgatgca acaaatacta gttatgaatt gcaaaaaagt
     5161 acgactgaag gtgtattaaa agaacttggt gtaaatgata aaaaacatat acttgtatat
     5221 aataaagtgg acaagctaga attggatata tatcctaaaa gtcaagaaga tattgtgtat
     5281 atttctgcaa aacaaggcat aaatatggat aagttattaa atatgataga aattgcttta
     5341 atggaaaata cttattcagt aagtttgatg ttaccttatg agagaggtga tatatttagt
     5401 agaataaaag ataaatataa tgtggagaat tttgagtatg gggaaagtgg tataacgcta
     5461 gatgtagatt tagatgaaga agattttaat atctatagag agtacatact agaaaaatag
     5521 gttgaatatg gatataaact gggaaaatat agataatctt gaagattatt ttataaccta
     5581 tcttctttat aaagaatcta aaacagtatc tcaaattagt aaaattagaa atatttcaag
     5641 tactgaagca agtgagcaac ttatacaagc aaagttaaaa ataaaggaaa tgcaaaaaga
     5701 tgattttgag gcatctaagg atattttgga taaatttcta gaactagata aaattaagag
     5761 acttgatttt atggatagct tagatgacga aaaaatggta tactttaaga gaaaggtttt
     5821 caaaagaatc cttgtagaaa aaaatgctga agatttaatt gttttaattt gggcgacagg
     5881 tgagctaaaa gatgataggt ttttgaaatt gcttcatcag ctcacaaacc acagacactc
     5941 agatattaga agaataactt attcagcaat cagaaagatt gaatcaccaa gtagtagaga
     6001 agtactgcaa aaaggtctct acgacaaaaa tgcacagact aggcagtact gtgcaaaagc
     6061 tctttcaaaa ttgggagatg aaaacagttt aaagatttta cagcagttga aagacaagaa
     6121 taagaatttt gaaaaagaat atgtgcttag agcttatgac aaagccatta agtcattgga
     6181 aaatgcaaaa aatttgaaaa agtaattttt tattaaagca atttgtttac ttaatgatgt
     6241 atttctaaag tttaacaagg taataaataa attaatggga ggggtttcaa atgatagtaa
     6301 gacgttgtgc aggtggagta gtgttttatg ctaataaagt acttattgta aaaaatgata
     6361 ggggtgaatg gacacttcca aaaggaaaga tactaggagg gggacttcca tatgagagtg
     6421 ctgttcaaag agttaaagta gaaactggca tagatgctaa aatgatagat gtggctggcg
     6481 atactatgta tgaatttttc tcaagaagta gacaacaaga agtttgtaat gcaataatgt
     6541 ggtatgttat ggaagcttgt aacactgatt gtgtacttgc tcctgaattt caagaaggtg
     6601 gattttataa agtaaaggat gctttagaaa tgttatcaca tcataaagag caagctttag
     6661 tagaagtatc ttataaaaag tttaaggaat taaagaaatt agtttcagag ggcgatgatg
     6721 ttccagcaaa ttaattgtga atttagagga ttctgaggaa gggatagccc cttccttttt
     6781 taattgaagc agttttaagc gtaaattata tagtctcttt aaactatgat ttatcaattt
     6841 agagatttag tagtttatat ttaaaatcag taagatatat ttaaaataaa aattaaaaag
     6901 tatacaattt tattatataa ttgtcttatg atgttgatta aagaattatt aaaagtccat
     6961 ttatttttaa atagaataat tttataaata tatatttgat gtgtatatta gaaaatgatt
     7021 aaaataggag gaaatacaat gagcaattac aggacattac atgaatttgg aatggatgaa
     7081 ataacgattg aaaagtctgt ttttattgga tatgctaagc ctataaagag tgaagaagaa
     7141 gcattagagt ttataaatga aataaagaaa aagcataaag atgcaactca caatgtgtgg
     7201 gcttatactg taggtaaaaa tatgaatata caaagatata gtgatgatgg agaaccacaa
     7261 ggaacagctg ggatacctac attagaggtt ataaaaaaag aagatttgag agatgtagcg
     7321 gttgttgtta ctaggtattt tggaggaaca aaattaggtg caggtggtct agttagagct
     7381 tatacaaaag gtgctaaact gggacttgaa gcaggtaaga taatttataa ggtaatgtat
     7441 caagaagtta aggttaagat agattatact caacttggaa aagtgcaaaa tgagttaatg
     7501 aatttggggt attttataaa agatactgta tatgaggata atgttgaaat agtagtatat
     7561 tcaagattag aagatgtaga gaaattatca gaaaaaatga ttgatataac aagtggaaca
     7621 ggaaaaatag ttttaggaga ggaattttat ttgtctgaac aaaatggaga aattttaata
     7681 taatctaaaa ggttattaga atagttaata cctcaaatag ccaattagta tttttaagtg
     7741 aaaagccatt aaattgattt aatctaaatc aatttaatgg ctttttatcg gattttgcac
     7801 agaatccttt ttttacttca aataaaccga ctaaaagtaa aaatatccca aatatttttt
     7861 ttaatatatc tgaagacaga taattagcta atagtgaacc aaatacagct cctaaaaccc
     7921 caaacacagc tataggtata accaatttaa aaattacatt tttatttttt atatgtatag
     7981 caagtgctat aagtgtcata gggatagatg aaagtaaatt tacgctttgt gctattttag
     8041 tgtctatgcc agcaagtaaa acgagtgcag gtataaggat tgttcctcca cccattccca
     8101 ttcctccaat aattcctgca aaaaatccaa ttagagcaaa taacattaaa acaccatcct
     8161 taatgctgca attatcataa ttattccaaa agaaattcta agaaactttc cagtaacctt
     8221 atttagtaat ttagctccta taataccacc aagaatactt ccaactgcaa ctttaattgt
     8281 taagttaaca tcataagtac ctttagatac atataaaaca gaacttgctg tcgttaagaa
     8341 tattattata gaaagagcag ttgcatgaga tttatgttct tctaccttaa cgatgtcatt
     8401 taatattgga actaaaagag tacctcctcc agaaccaaaa actccattta taaatcctgt
     8461 aaaaactcca attatcgtat tttttaagtt aaaatcaaag tattttttct ttaaatcatc
     8521 agtttttttc ataaaaatca ccttctgaaa tagttttacc aaaataggca aattatattt
     8581 atataaaata agattaaata taatctttca tgtttgttta attttgcata aaaaaaaaca
     8641 tattaaaaca acatattaat atatggtata atatattaaa acaaagtagc aaatttgaga
     8701 ggagataaca atgtcaaata taaaaataca aatagacaaa actgtagaat ggcttataaa
     8761 taaggttaat gaggcaaatg caaaaggatt aattgttggt gtatctggag gaattgattc
     8821 ggcagtagtt gctaatttaa ttaaaaaggc atttccagaa aactctatgg gagttataat
     8881 gagcataaaa agtaatcctc aagatagaga agatgcactt aaggttatag agggatgtga
     8941 tatagaatac cttgatttag accttataga accacaaagt gctatattag acatggtagt
     9001 aggaaattta aaagataaac atttatatag agaagaatat ttaaagatga cagatgcaaa
     9061 cttaagagca agagtaagaa tgagtacaat ttatacaatt gctaacaatc taggatatct
     9121 ggttgttggt acagacaatg ctgcagaaat acacacagga tattttacta aatttggtga
     9181 tggtggagtt gatattttac ctattgcaaa tttaactaaa ggagaggtgt atgaatgggc
     9241 aaaagagctt ggtgttcatg aagatttaat aaacaaagct ccttcagcag gtctttggga
     9301 aggtcaaaca gatgaagatg aaatgggaac tacttacaat atgatagatg ctgtgttaga
     9361 aggtagattg gatgaagttc ctaaaagaga ccaagagata attgaaagac ttcatagatt
     9421 aagtgaacac aagagaaaaa cgcctgcaca acctcctaag ttctaattta ctatttataa
     9481 gatttgtgat aaaatttaca agtgatgaaa attagaataa ttaattgtac gtattaagga
     9541 gtggaaatat gggacgtata ggtaatataa ttaatagaaa aggtaaacaa gatgctcaaa
     9601 gagcaaagat atttactaaa catgctagag caatagcagt tgcagctaag gaaggtggag
     9661 cagacccaga gtacaatgca gcgttaaaaa cagctataga aaaagctaag gcagataata
     9721 tgccaaatga taatatagat agagctatcg ctaaaggtgc tggagcaggt gctggagaag
     9781 actatgaaac tatagtttat gaaggatatg gacctggtgg agttgctgtt atagtagaaa
     9841 ctttaactga caacaaaaac agaactgctg gtaacgtaag atactatttt gacaaaaacg
     9901 gaggaaactt aggaactagt ggatgtgtgt cttttatgtt tgataaaaaa ggacaaatat
     9961 tagttggttt aggagatgga gtttcagaag aagaattgat ggatgttgca ttagaagcag
    10021 gagctgaaga tttcataaca gaagaagatg gttatgaaat aataactaca ccagaagatt
    10081 tttctagtgt tcgtgatgag ttaaaagcta aaggatatga gtttatatct gctgatgtga
    10141 agatgatacc tcaaactaca actgtattga ctgaagaatc tcatttaaaa atgatgaata
    10201 aattagttga tatgcttgaa gaagatgatg atgttcaaga tatatatcat aactgggaag
    10261 tagaataaat agtataagca tattgaaatt tataagtatt gaatcaattt taacatgtga
    10321 taaattttat agaataagaa acactaaaat agacatacgc acctctaacc tatatttgca
    10381 taataatatg taagcatagg taaggggtgt ttttatattt ataggttaag aaatttaaag
    10441 taaattttga gctggactcg attgtctata aataattgat gggtatataa cttgttgctg
    10501 aataccattt atacgcccag ttactgtatc aataatcatc ctaagactac gttctgctgt
    10561 ctcttccatt tttaaatcta taactgtcaa aggaggaata cttcttgcca ttagtcgtga
    10621 aggtccatta cttgctgcaa atatttgaat atcctctcca attacaattc ccgattcatg
    10681 aaatgcattc atcataccaa gagctaccac atcatgatta caaaaaatta ctttttttag
    10741 tgaatttttt gcaagcattt tttgagcaat atcatatcca ctatctattt tattagaaca
    10801 ataatatata gaatctgata gatgtattcc atgttcttta catgttttta aaaaagattt
    10861 accccttaaa gtcatacatg taaaggaagt tgggttgaat acaacagcaa tatcattttt
    10921 accattttga atagcatgta aagctgctat acgaccagtt tcctcattat caatagatac
    10981 acttgaaaaa ccaggtattt cacgatttac aacaactaca ggaattttag gtttattttt
    11041 tactagatat tctatatcat cagcattgat acctgttaaa acaattccat cataataatt
    11101 atttgcaaaa aagtattcat catcacatag atgaccaact ttgtaaggtc gtatgatgat
    11161 atttatgttt aaatgttcta aagaaattat gtggtttatt gcttgaagta tattggttga
    11221 tgccaactca aatccctcat cctgccaata aaaagctatt ttaatattat tatgtttaat
    11281 aggagtagag cgtttatatc caagcttatc agctgaattt aatataagct cttgtgtatg
    11341 tagcggaatc ctgcgttcat ttcctttacc acttaagacg tacgagacag ttgctgaaga
    11401 aagaccacag tcttttgcaa tatctttgat tgttgccatg ataaaatcct ccttaaatag
    11461 ttgcttaaat ttgcttatac caataatctt aacatatatt caactaaaga acaatattct
    11521 acaaggtgca tattttacag aattagaata aataaattta aaaaaacttc ttacttatgt
    11581 tgacataatt tagtttatag gattataata tgcttaaata agcttattat taacttaaat
    11641 taagcaaagt taagcaggaa catgcaatgc tagcttgcaa tgtgactgta aagggggaaa
    11701 aggtatgagt aaggtaaata tatttgaagt tggtcctaga gatggatttc aaaacctgaa
    11761 agagttttta gaaactgata aaaagcttaa gattattgat ggtcttattg cttcagggat
    11821 taaacaactt gaaattagtt catttgtaag cccaaaagca atacctcaga tgaaagatgc
    11881 aaaagaagta gcaacttatt gtgtagaaaa atatcctgat gtgaaacttt atgcattagt
    11941 tccaaattta tacggtgcta aagttgcatg ggagtgtggt ataagagata tatcctatgt
    12001 tatatctttg agtgaaactc ataataaaaa aaatataaat cgtacccatg aagagtctat
    12061 tgatgagctt aaaaaaataa tgaatactta tccagacatg aatatatgta ttggaatggc
    12121 tactgtattt ggatgtcctt ttgaaggttt accaaaaatt gaagatgcca taagatttgc
    12181 taaacgcctt tgggaaaacg gtatcaggga gatgaatcta gcagatacaa tagggattgc
    12241 tgaccctaag caagtaagag aaactattag tgcattagtt gaagctttac cagagtgcag
    12301 atggcaggta catattcatg atactagaaa tatgggaatg gttaatactc ttgtggctat
    12361 tgaatctgga attactactg ttcagtctac acttggagga ttaggaggat gcccatttgc
    12421 tcctggagca tctggcaata ctgcaactga agatttagta tatatgcttg aacgtatggg
    12481 gtatgataca ggagttgatt ttgttaaact tcttgaatta tcaaagtatc agaagagttt
    12541 tgtaaatgga gtatatagtg gtcatcatgt aaatataaag tctgatagtt gtaattgtta
    12601 gttctattga ttaatcgatt aaaaaaatat gttgattaaa aaaatatgct gattggtaaa
    12661 atagatttat tgataaaaaa tataaattat aaaatacatt atctaataaa acaaataaaa
    12721 aaattgaggg ggattagtat ggatattata gctttacttt tatttttagg agttattatt
    12781 attgcatttg tacgtaagaa taatgcaggt attttggctc tagctattgg tgtcatagct
    12841 gttagaattt ttggaatgga tgataaagaa ctttatggag ctataagcaa ctcaatgttt
    12901 actacacttg ttggtataac actactcttt gcaatagtga actcaacagg agcattaaag
    12961 cttgttgcag aaaaaattgt agctgcttct ggtaaacgtg tttggcttat acctatagca
    13021 gtttttatag caggatttgt tgttgctggt gttggacctg gggcaattcc agcacttgca
    13081 attatcccag caatggctgt tcctgtagcc ataacggttg gttataatcc tgtaatgctt
    13141 gcacttatag gtgaatgtgg attaatggca ggtcgtatga ctcctattac tccagaaggt
    13201 caaattataa caaccgttgc agaaggggtt ggaattgaaa atgttatgcc tactatctta
    13261 gcctgtcaaa ctctttctac aattgtattt tcaatagtat tgtttataat tttcaaagga
    13321 tataaactta aaaaacctat aaatgttata gatagtaagc aaatagaaaa attcaatata
    13381 aagcagataa tttctctaat aagcatagta gttatgattg cttttatcat aatttttgat
    13441 atgaatgttg ggcttgcagc ttttgcagtt tcagctgtac ttattttttt caatattgct
    13501 gatgaaggag aatgtatcaa atcaattcca tggagcacga tagtaatggt tcttggagta
    13561 ggtgcaatga tgacaatcgt agatgcagca ggaggtattg acttgatgag taatgcatta
    13621 tcatcactga tgaatagtaa aacagctact ccaattatga gtatttcagc aggacttatg
    13681 tctatggtta gttctgcttt agcagtagtt tatccaacta tgatgcctat gtgtgttgat
    13741 atagctaagg cagttggagg agtagaccca ctagcactta ttgctgcagt tggtgttgga
    13801 ggttctcttg ctggtgtaag cccactttct actggtgggg cattgatttt agcagctatg
    13861 ggttcaagta agaaagactt taataaggaa gaacaaaata aggtttttgt acagcttttc
    13921 attatgagtg cagttggctt attggttatt gcagtagtat caatattatc atttaatgca
    13981 ataacacatt taatgaacta attagaataa ctgaaaataa atcattaaat ttaactttta
    14041 atattaatca atataatata atttggaggg gaagttatga aaaaaggagc attaagcaat
    14101 ataaaggtac ttgaccttac aagagttctt gcaggtccat attgtacaat gatgttggcg
    14161 gattttggag ctgatgttat aaaaatagag atgccaagaa aaggtgatga tacaagaggt
    14221 tttggacctt ttgtaaatgg agagagttta tactatgcaa atataaatag aaataaaaaa
    14281 ggtatcactc taaaccttaa atcagaagaa ggaaaaaata tatttttaga aatggtaaaa
    14341 catgctgatg ttgtagtaga aaattacaga ccaggtgtaa tggaaaagtt aggtctgggt
    14401 tatgatgagt tggctaaagt taacaatcaa ataatttatg gagctgtatc aggatttgga
    14461 tgttatggac catattcaga acgtccaggt tatgatatta ttgcacaggc aatgggtggt
    14521 cttatgagta taacagggca agaaggagga gaaccaacga gagttggtaa tgctatgggt
    14581 gatgtactag gtggaatgaa tttaactatt ggtatactca cagcactcaa cgctagaaaa
    14641 ataataggaa aaggacaaag agtagatgtt tccctcgttg attctgttgt atcgtctctc
    14701 gaaactggta tacagagata ctttgattca ggacaacctg aacttatggg taatagatat
    14761 gcttcagcct atccttatga ctcttttaag gcaagtgatg gaagttttgt aataggctgt
    14821 ggtaatgaca aactttttga aaagttatgt aaaaaagttc tttttagaga agacctttta
    14881 atagatgaga gatttaaaac aaatcttaat cgttgtgaac attacaaggc tcttaaacca
    14941 gaaatagaaa aatggtctat taagcataca ataaaagaag cagttgactt aatatcagag
    15001 gctggagttc ctgctgctcc tatctataat cttgattgtg ttacaacaga tgaacatata
    15061 gcaaaagcaa gagaaatgat tgtagatatt agtcatccta ttataggaaa tatgaaagtc
    15121 aatggaaatc cagttaagtt gatgacaaca aaagctaaaa ttgatatgcc agcaccatct
    15181 ctaggggaaa gtaacgagga gatttataat ggttggcttg gttttgatag tgataaaata
    15241 aaagatttaa aagaaaatgg agttatttga ttaaaaaata gctaaacaca cttgtgactt
    15301 tagtcagggg aggttcagtt agaataatat caaaaaatat taaatcaata ctgtaaatca
    15361 aatatagaat tgtcagaagc atttaagatg ggaaaagcta cttagacatt gagtctttgt
    15421 ggcttttcct attttcatta agaattttat attatcagaa tagatatgta ctaaactaaa
    15481 gtattaacaa tttaaattaa tagaaaataa actacctaat tatgttgaat aaagactact
    15541 ttagtgaaaa taaacttata gagatgctat ttaaattatt ttaatggaaa tatcaaaaat
    15601 gagactgcat tctcaaaaat gagactgcgt tttttaagta ttttctcgaa aatgagacaa
    15661 aaatattgtt ttatgggttt tattgaagaa aaattcttct gtgaattaca atttaatcaa
    15721 ttttaaagtt gaaaattatt aaaaatttag tgaaatttat gtatatttcg tttattttat
    15781 taaaaataac aattttttac taaaaataaa gaacttcaat ggtgattggc atagtacttg
    15841 ctattttata ttaacaagaa atgtagaaaa aataacaatg tataatgggg ggaatatgat
    15901 ggaatctgta atggaaaatt tgaacaatct taaggtggag gttaaggaca aagtctgtgt
    15961 tattactata aatagaccta aagctttaaa tgctcttaat agtgacactc ttagggaact
    16021 atcacaagtt attgatgtag tatcagaaaa tgaggcaata ttaggagtta ttattactgg
    16081 agaaggtaaa gtttttgtag ccggtgctga tatacgccag atgcaaaact ataaaagtga
    16141 ggaaggcaga aaatatgcag gttatgctca gggagttttt gataaaattg aagctcttga
    16201 aaaaacagta atagcagcag ttaatggata tgcattaggt ggtggatgtg aacttgctat
    16261 gagttgtgat attagaattg catcagaaaa ggctatattt ggacaacctg aagttaattt
    16321 aggagttatt ccatgttttg gtggaactca aagattatca agacttgttg gaactggaat
    16381 agctaaagag ttaattttta cagggagaca agtaaatgca gaagaagcta aatctattgg
    16441 gcttattaat aaagtggttc caagtgattt acttttagaa gagtctatga agatgatgaa
    16501 ccaaatagtt gaaaaagcgc caatagcaat aagatatgca aaagttgtta ttaacaaagg
    16561 gatagatatg gatttaaaga atgctcttga actagagaaa gatattgcag gtttaacttt
    16621 tgctacaaga gataagcaag aaggaatgaa tgcatttata gaaaagagaa agccagtttt
    16681 tgaaaataag tagctattat aactaaaatt ggaacaatgt agaatatatt caaaatctaa
    16741 atttcgtgaa aaaatacata tagtttaatt ttaaaaggag ttaatttatg aatacgggag
    16801 taattataag tttagtaggg attatcatag ctctatctct tctagttgta atggttatga
    16861 agggaataaa tatttttatt atagcaattg tatgttcatc attagttgct ataacaggaa
    16921 gcctaaatgt gtatgatgca cttaaagtgg attatatgac agggtttgtt ggatttcttc
    16981 aggcaaattt ctttattttc ctaacaggga cattaatggg aaaaataatg gagattactg
    17041 gtggagcaaa atctattgct aagatgattg taagatggat tgggaaggat aaagcactac
    17101 tttctattcc aatagcttgt ggaatattag cctatggtgg agtaagtgtt ttcgttgtaa
    17161 gttttgcagt gtttccgata gctttagaaa tttttaaaga agctgatttg ccacgaagat
    17221 ttataccagc agctttgact tttggatgct caacttttgc tatggttgca ccaggggcac
    17281 cacaaattca gaatattgtt ccagcatcaa cattagggac agatattatg gctggagcag
    17341 taaatggttt tatatcatgt gcagtaatgt ttatagttgg aagtacaatt ttgtacaaaa
    17401 tggtttcaaa agaaaaagcc aatggtggtc attttatagc acatgaatca gatatatttg
    17461 atgaagcaga agcaactaat tctaagagag aaaatggtcc caatggtgta attgccttaa
    17521 ttccattgat agtgtcaatt ttaataatta atgtaaaagt taatggaaaa gcgatagtac
    17581 caattgaggt gggagtattt attggagctt tattagtata tttattgtta aataaatatc
    17641 aagataatac taaaatagtt ggacatgttg gtgatgcatg taaaactaca gtagtagcaa
    17701 tatgtaatac ttgtgctgta gttgcatttg gttcagtagt aaaatcagca gttggttttg
    17761 acttcatagt taatgctatg actaatattc ctggtcctcc tattgcaggg gctgcagttg
    17821 gaactacagt tattgcaggt atttgtgggt cagcttcagg tggactagga attgcagctc
    17881 cacttcttgg accagtattt ttagccaagg gtgtttccgc tgcagcatta actaggacta
    17941 tggcaatttc atcagcagca ttagattcac ttccacacaa tggatatatt gttactgtaa
    18001 caaatggtct ttgtaaagaa acacataagg atgcctatat gccaatattc tgggtaactg
    18061 ttttaacccc atttattgga accatagtag cagtcctact gtttacaatg ttcccgggat
    18121 tgccataaga gaaaagataa aaggtataaa ttatattttg atgttataaa atcaattttc
    18181 aaaatatata caataaattt catgttttaa tactgtaaat atgtatttaa aataacaaat
    18241 tgaccatata aggaggaatt gaaagtgtta aataaaccat tagagggagt taaggttatt
    18301 gacctaacgt attttgtagc aggtccaggg acttctaaaa tattagctga ctggggtgct
    18361 gacgttatta aggtagagcc aagctttgga gacccaggaa gaaagacagg ggcaacaatg
    18421 acaatgccaa tagatgatta taataatcct ttttacagca catataattc aaataaaaga
    18481 ggtttgtcta taaatcttaa gagtgaaact ggaattgaaa ttatggataa gcttttaagc
    18541 gaagcaaacg tatttgtttc aagttataga acaggagcat taaaaagact aggtcttgat
    18601 tatgaatcat taagtaaaaa gcatccacac cttatctggg gtcagataaa tggttttggc
    18661 gattttggac ctgctaagga taatgcagga tttgatacag ttgccttttg ggcaagaagt
    18721 ggggctatgc ttgatatagc agaaaaagat acaagtccaa taaatccacc tatagccttt
    18781 ggagatgcaa caacttcttg tagtttatct ggaggaatat gtgcagcttt gtaccagcaa
    18841 gctaagactg gaaaaggaca aaaagtaatg gcatctttat ttgggcaagc aatatggaat
    18901 gcatcatctt tattagcatc tacacagttt agtgatgagt atccaaaaac tagaaaaaat
    18961 gctatttcac cagtaataaa ttcttatcgt tgtaaggatg gaaagtggat attcttaagt
    19021 attttagagc atgaaagata ttttgaggca ctttgtagat tatttggaag agaagactta
    19081 ataaaagaag agaaatttgc atcttcagta gaaagtaaga aaaatgctac agaacttatt
    19141 aatattttag atggtgaatt tgcaaagttt actcaaaatg agatggttga aaaacttact
    19201 gctgctgata tagctcatga aaaaattcaa catgttaatg atgtagtgac tgatgaacag
    19261 gctattgcta ataactatat atatgaacat acaagtaaag atggaaataa gacaatgctt
    19321 gcatctactc cagttaaatt tggaaatatt gaagttaaca tgacttgtga tgctccactt
    19381 ataggagaac attcagatga gatattgaga gaacttggat acagtgattc tgatatagag
    19441 gctctaattg aatctggaat tgtaacaatg aataaagaag catgtgaaat atgttaataa
    19501 acatgtgaag tatgttaata aataggaggg aattttatgg attttagatt aacagaagca
    19561 caattaatgc ttcaaagagt agcaaaagaa tttgcagaga atgaaatagc accaatagct
    19621 gctgaaacag ataaaacagg tatttttcca agggagcttt ttagcaaaat ggctaaaata
    19681 ggatttaatg gaattggaac acctgtagag tatagtggtt ctggtggggc agatatagaa
    19741 aaggtaattg ttgttactga gattgccaaa aaatgtgcag catctgcagc aatattatca
    19801 atacacacaa tatatgctca agctattttg aaatttggta ctgaggaaca aaagaaaaaa
    19861 taccttccaa tgatggctga aggtgggtgt gtaggtgctt ttgccttaac cgaacctaat
    19921 gctggttcag atgctgcgag agctgcaaca actgcaatta ttgatgaaga aactgatgaa
    19981 tatgttttaa atggaactaa gtgttttatt tctggtggag gtcaagcaga atcattaata
    20041 atatttgcat tgacagaccc atcaaaggga ataaagggaa tgtctgctat cattgtcgac
    20101 aaaggtacgc caggtttttc aataggaaaa attgaagaaa aaatgggtat tcatggttca
    20161 gaaactgcag agttaatatt tgataactgt agagttccaa agtctaactt attaggaaaa
    20221 gaaggtaaag gatttaatat agctatgact tgtttagatg gagcaagaat tggagttgga
    20281 gctcaagctg taggaattgc tgaaggggct ttagaagaaa gtatcaaata ttctaaagaa
    20341 agagtccagt ttggcaagcc aatttcagct ttgcaaggta ttcaatggta tatagcagat
    20401 atggcgacaa tggtggaatc agcaaaatta ttagtatatt atgctgcaga tttgaaagct
    20461 agaggagaaa aacacactaa agaagctgct atggcaaaat acaatgcgtc acgtactgca
    20521 agagaggtta caaatttagc ccttcaaatc catggtggat atggatatat gaaggattat
    20581 ccattagaga gaatgtacag agatgccaaa ataacagaaa tttacgaagg tacttcagaa
    20641 atacacaaag ttgttatttc aagagcagta ctaggatagg aggaaaagaa tgaatatagt
    20701 tgtttgctta aagcaagttc cagatactaa tgaagttaaa ataaataaag agacaggtac
    20761 tctaataaga gatggtgtac caagtataat caatccagat gatagaaatg ctttagaaga
    20821 agctctaaaa atgaaagatg aactaggagc agtgattaag gttataagca tgggacctcc
    20881 tcaagctaaa agtgctttaa aagaagcttt ggcaatggga gctgatgaag cttatttaat
    20941 ctcagataga gcttttggtg gttcagatac ttgggctaca tctacaatta ttgctgctgc
    21001 tatagagaag gttggaaagt atgatgtaat attctgcgga agacaagcta tagatggaga
    21061 tacagctcag gttggaccag aggttgcaga atttttaggg ataccacaag ttacttatgc
    21121 gaaagaagta aaggttcaag atgacaaatt actagtaact cgttatacag agacagggga
    21181 ttatttaata gaagcaaaga tgccagtttt attaactgct ataaaagagt taaacaatcc
    21241 aagatatcca agtgtcaagg gaatcttaga agcttataat aatggagatg caaagattac
    21301 tgtattaaca cttgcagatt tagatgttga tacaactcaa attggattaa agggttcacc
    21361 tacaaacgtt tataagtctt ttgtaccagt taaagacaag cataatgaaa taatagaagg
    21421 tataaataaa aaagaaaaag ctgagaaact aatagaaata ctattcgatt tgaaactagt
    21481 ataggagtgt taagatgatg agagcaaaag ttaatcaagg tataaattta aatgattata
    21541 atggtgtttg ggtaatagga gagcaaaggg aaggtaaaat taatcctgtt acgatagaac
    21601 ttattggtga aggaagaaag cttgctgacc aattaggaaa agagcttgca gttgtgatag
    21661 ctggatatga agtagagaaa gaagtgaagg aattacttca ctatagtgtt gataaagtat
    21721 attacatcaa tgaccctctt cttaaagatt tcactactga tggatatgca atttcaattg
    21781 caaatttaat cgaaagaaaa aagccagagg ttgtattggt tggtgctact tctataggaa
    21841 gagatatagc gcctagaatt gcaggtaagg ttggaacagg tctaactgct gactgtacaa
    21901 aacttgaaat agattcaaca gataataagt tattacaaac aagaccagca tttggtggaa
    21961 atttaatggc cactattgtt tgtccaaaaa atagaccaca gatgtcaaca gttcgtccag
    22021 gagttatggc aaaagctgta agaaacgaat cagagactgg aattttagaa gttgttactc
    22081 cagaacttac tgaaaaaatg attagaacta gattagtaga gatacttcca caagaaaaga
    22141 aatctgtaaa tctaactgat gctagaataa tagtatcagg aggaagagga ttaaaaagag
    22201 cagaaggatt tgagcttata aaagaattgg ctgataaatt gggggcagag attggtgctt
    22261 caagagcagc agtagattca gggtggatag aacattctca tcaagtggga caaacaggaa
    22321 ctactgttag accagaacta tacattgcat gtggaatatc tggagcaata caacatttag
    22381 caggtatgag tgattctaaa tatatagttg ctataaataa agatgcaaaa gcccctattt
    22441 ttagtatatg tgactatgga atagtaggag atttatatga aattatacct gaaatgatag
    22501 aatcattaaa taggtaactt ttagaaaaca ttgaatatgt agaatgataa gtcttataat
    22561 attgtattgt aagacttatt atttttgctg aaggttatag tggggagatg aaacaatgaa
    22621 agagcagtgg tataaggata tatttgcaag agtattatct atgactgatg atggttttat
    22681 tgttgtaaat agaagtggag taataataga tattaatgat aaatattgtg actttttagg
    22741 aaaagaaaga aaagatataa taggacaaaa tatacaaagt attataccaa atacaaaaat
    22801 gctagatgtt atgaaaaata agtattgtga agaaggagct atacatcatt attcaggtgg
    22861 aaatacaaaa gaaaaaagtg taatagtaag taggtcttat gtggaaaatg acaatggaga
    22921 agtagttgca ggagtagctc aagttaaatt tagattgcaa tcatttgatg tagctaaaaa
    22981 gttaatgtca gaatatatgg agctccaata ttataaagag cagtttaagg ataactgtgg
    23041 ttttgacaag ctcataggtg aaaatagaga cttcatagaa cttaagaaaa caggtgtgaa
    23101 agcttctaaa acaaactttc cagtactttt aacgggtgaa acaggtacag gaaaagaagt
    23161 ctttgctaga gcaattcaca ataatagcag taggtcagat aaacctatgg ttagtataaa
    23221 ctgcgctgca ataccagaag aattattaga gtcagagcta tttggctatg acgaaggagc
    23281 ttttacagga gcaaaaaagg gtggtaaaaa aggtaaattt ttagttgcga ataatggtac
    23341 aatcttttta gacgagatag gtgacatgcc tttgaccatg caagcaaaac ttttaagagt
    23401 tcttcaggaa agtgagatag aaccagtggg aggtcttaaa actataaaga tagatgtaag
    23461 agtaatctct gccacaagaa agaatttaag taaaatggtt gaagagggat tatttagaga
    23521 agacctatac tatagattaa atgtaataaa cattcatatg atggaattga aggacaggca
    23581 agatgatatt ttattgctag caaattatat attaaacaaa ttaaatgttg aatacaagga
    23641 attgaaggtt ttaagtgata aggttaaaaa ttgttttatt aactatactt ggccgggaaa
    23701 tataagagaa ctacaaaatg taataaaaag tgcctatgct gtaagtgatg atatggtcat
    23761 tatgatgtgt gaccttccat ctaagatgga taatatcagc agagtagctc aatgtaatgt
    23821 ggatagcaat tgttcaattc atgaaatggt tgagaattat gaaaagagct taattattga
    23881 tgtgttaagg aagtataatt ggaaatgttc taaggcagca gaggttatgg gaatacacaa
    23941 gagtctttta tataagaaaa taaaaaagta tgaaattgaa ttaaataatt aagataaatt
    24001 gaattaaggc aatgctaaaa aaacgccctt attcaaagcg aactgtataa actgttttat
    24061 gcagttaaaa cgtgtaaaag tagatgtgaa gtactaatgt caatcattag tactttttta
    24121 ttatatttat aattactatc tatatttcag tatcttttga tatctaagat tattatgttt
    24181 aatttaggat ataacaaaac cctcttagtt atggacagtt ttttatattt taactgtcta
    24241 ctaagagggg atagtttagt ttaaatttag agtaaatttt atctagcatt tataaaaaat
    24301 attttgaata gcttttttgt tatttaatca taattttcta cttctttatc aagttctttg
    24361 ccacaagaat cacacttata tatagcattg tatccgtaat catattcatc aactattttc
    24421 ttttgtctaa atacaccacc acactcacat aggaaaccaa tttttataag tgtaacgata
    24481 aattgtgatt ttataaattt tatatcatta catttatcgt tagtgtaaaa tgtataaata
    24541 taagagttat cgcactcatc tcttaattca aatctttcaa tatatgtgtt attttttaat
    24601 tcctcaaatt cttctttagt taaatatgat ttttgttgca attcatgtaa acgcttaatt
    24661 tggtccatta tttatatcct cccaataatt ttagtatttt aagcttagat aaggaatcta
    24721 agttttatta aatttatccc aaggaattat ttaaatcata tttagtttta ttaattacaa
    24781 catatataaa ttacacgaaa ataaaactaa ttgtaaaaat aatatcctgt gaaaacctaa
    24841 actactaacc ccttactaac ccgaaaacta aaaaaagaaa atatatccaa atataagtga
    24901 tatataaata aagatgtttc atataaattt aactttaaat att
//
LOCUS       DBGJFU010000051        24674 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.051, whole genome shotgun sequence.
ACCESSION   DBGJFU010000051 DBGJFU010000000
VERSION     DBGJFU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.051"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            95..799
                     /locus_tag="ACOIAS_001960"
     CDS             95..799
                     /locus_tag="ACOIAS_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2701672.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAISRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            801..2288
                     /locus_tag="ACOIAS_001961"
     CDS             801..2288
                     /locus_tag="ACOIAS_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2701673.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            complement(2399..2962)
                     /locus_tag="ACOIAS_001962"
     CDS             complement(2399..2962)
                     /locus_tag="ACOIAS_001962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701674.1"
                     /translation="MGISKCTSFLNRQIILQVNKKTILDKIVANTLILLFSSIISKPS
                     FTNKTKILVTTISDSIKNILNKTVVNNRVFKFPLYKNDVEKIRMKIKGIVILGFKVIK
                     LQNPKHIIKPSLAYLTTFIYPSFWPKKSDKNIWMTKASNTTVTKFVILKISCSFIHIF
                     YKIRSLLKPKYTSDFTFPFVSIFKHLL"
     gene            3156..4106
                     /locus_tag="ACOIAS_001963"
     CDS             3156..4106
                     /locus_tag="ACOIAS_001963"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2701675.1"
                     /translation="MKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKS
                     FVSQAWTEKFGYNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLK
                     KSNGEYIWCRTSITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNK
                     GTFEKLVEETIVMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFG
                     SKDLVGRIGGDEFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIAR
                     YPQDGTSFFELFKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4469..5299)
                     /locus_tag="ACOIAS_001964"
     CDS             complement(4469..5299)
                     /locus_tag="ACOIAS_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2701676.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            5372..7099
                     /locus_tag="ACOIAS_001965"
     CDS             5372..7099
                     /locus_tag="ACOIAS_001965"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701677.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7136..8998
                     /locus_tag="ACOIAS_001966"
     CDS             7136..8998
                     /locus_tag="ACOIAS_001966"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701678.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            9331..10020
                     /locus_tag="ACOIAS_001967"
     CDS             9331..10020
                     /locus_tag="ACOIAS_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HGZ2701679.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYQIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10137..10811
                     /locus_tag="ACOIAS_001968"
     CDS             10137..10811
                     /locus_tag="ACOIAS_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HGZ2701680.1"
                     /translation="MTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMVYKKT
                     YMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDPMDLV
                     FLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNEDSEDI
                     ATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAVLVSY
                     NGDYVA"
     gene            10828..12735
                     /locus_tag="ACOIAS_001969"
     CDS             10828..12735
                     /locus_tag="ACOIAS_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HGZ2701681.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPIEVGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13186..13875
                     /locus_tag="ACOIAS_001970"
     CDS             13186..13875
                     /locus_tag="ACOIAS_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2701682.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            14311..14850
                     /locus_tag="ACOIAS_001971"
     CDS             14311..14850
                     /locus_tag="ACOIAS_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGZ2701683.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            14869..15912
                     /gene="potA"
                     /locus_tag="ACOIAS_001972"
     CDS             14869..15912
                     /gene="potA"
                     /locus_tag="ACOIAS_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HGZ2701684.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            15914..16750
                     /locus_tag="ACOIAS_001973"
     CDS             15914..16750
                     /locus_tag="ACOIAS_001973"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701685.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            16855..17529
                     /locus_tag="ACOIAS_001974"
     CDS             16855..17529
                     /locus_tag="ACOIAS_001974"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701686.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            17529..18581
                     /locus_tag="ACOIAS_001975"
     CDS             17529..18581
                     /locus_tag="ACOIAS_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2701687.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            18811..19659
                     /locus_tag="ACOIAS_001976"
     CDS             18811..19659
                     /locus_tag="ACOIAS_001976"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2701688.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            19649..21313
                     /locus_tag="ACOIAS_001977"
     CDS             19649..21313
                     /locus_tag="ACOIAS_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HGZ2701689.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            21362..22618
                     /locus_tag="ACOIAS_001978"
     CDS             21362..22618
                     /locus_tag="ACOIAS_001978"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGZ2701690.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            22641..>24674
                     /locus_tag="ACOIAS_001979"
     CDS             22641..>24674
                     /locus_tag="ACOIAS_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGZ2701691.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSMKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIV"
BASE COUNT         9739 a         2449 c         4238 g         8248 t
ORIGIN      
        1 aaataagatt tatcttatct attgggattt aattatttaa tcaatatatt tataccattt
       61 aatttaaata aaaagtctat taaggagtga gcttatgagt aagttaattt atatagcaga
      121 tgatgaagat aatataagaa atttggtaaa gacattctta aaaaatgaag gtcatgatgt
      181 tatggatttt aaaacgggtg atgaactttt agaacaattt aatataaagg agtgtgactt
      241 agtaatacta gatataatga tgcctggctc aagtggtttt gaagtatgta caaaacttcg
      301 tgagaaaagt acagtcccaa taatcatgct tactgcaaga gatacagata tagattatat
      361 tacagggata accttaggta gtgatgatta ttttacaaaa ccatttagcc caatgtcttt
      421 ggttatgaga gtaaaatcta tatttagaag gatagagttt gaaaagaaac aaaattatga
      481 taaatatagt aactcgatag atatggaatt aaaatttggt gatgtaataa taaataaaaa
      541 gaataaaatc gttacaagta aaaatgtaaa tatagacctt acaccaaatg aatataacct
      601 attgacttat ttgtttgaaa atatagatag agcaatttcc agagatgaat tattaaacaa
      661 aatatgggga tacgatatag aagttgagac aagggctgct gatgatacag tgaagagatt
      721 acgtaaaaaa attttagata caaatatttt aatagaaact gtatgggggt ttggatttag
      781 gttgaaggag aaaagttaag atgaagaatg gaaatgacaa gaatagaatt ggaaagatta
      841 aatccaaatt taaattaaag ttctcaaaga aaaatataag cataaaaact aggattttgt
      901 tgagtatact ttgtttgata attatagtat ttatgataat attagtatct tttaatgtat
      961 ttcttgatac atatataaaa acaactgcga atgaagaatt gacaaagtct acagaaatag
     1021 tagaacatat ggatagtaat tttagaccta ttaagccacc acaagatgat ggaaagcttc
     1081 ctcccaaagt attatctaat tttatgagaa atgtacaaga taaagttagg atggcagaaa
     1141 cacaatcaga tgctgatgct atggtagttg attctaagta taacctgata tttccaacaa
     1201 gagaggacga tttcttaaaa aatgttgatg agatggagct gattagggag tgtattcaaa
     1261 atgaaaaatt agatttaaat tcagatgcaa atacacggat atctaccaga aataaggatt
     1321 attatatttc aactgtaaaa attaccagta tatatggtga acaagacaag tatcttatac
     1381 tttttataga catatcaaaa actctaaatc tagcacaaaa gataaatatt gtattaatat
     1441 ctgtaatgtg ttttgctgga atattagcaa tatttacagc tgtgatttta tctgagaaaa
     1501 ttgctaaacc gataaaagag ttgtgtgagt ttgcaaaaaa aatgggtcaa ggagatttca
     1561 aaagaaccta ctttgacttt tcagataaag aattagtaga gctctcaact gttatgaata
     1621 agagtgctga gtatctggat aagtatgata atgaacaaaa aatatttttt caaaatgcat
     1681 ctcatgaatt aaggacacca cttatgtcta ttaaaggtta tgcagaagct ataaaatata
     1741 atgttataga tagtaaacat gcaagtgata taattcttga agaaagtgat aggttaagtg
     1801 atatggttga agatttatta tacatttcta aaatagataa tataactaaa gattttgaac
     1861 ttgtagaatg tgatttaaga gaagtacttt caaactgtgg agctaggcaa aatgtaagag
     1921 ctataaataa ggggataaag tttatttatg aatttgatga agaaatggtt ttatttgaat
     1981 gtgatgaaaa aaacatttcc aaagcattta tgaatcttat agaaaatgct ttgagatacg
     2041 ctaagagtga aataaaaata gtgtgtaagt ataatcaaaa aaatatagtt gtgataatag
     2101 aagatgatgg aattggaatc aagaaagaag atttacctca tgtttttgaa agattctata
     2161 agggagtagg aggaaatcat ggaattggat tatcaatagt taaatctatt gtaaataagc
     2221 atggtgggcg tatatacgta gaaaatggca aaaaaggagc taaatttact atattattta
     2281 aattgtaaaa aaattagaga aaatatagag atgtttgaag ttgtttttta tatatatctg
     2341 ttgtatactg ttgaaaaaag aacaatatta agctgacata tattacgata gttgacattt
     2401 aaagtaaatg cttaaatata gatacgaaag gaaaagtaaa atctgaagtg tatttaggtt
     2461 taagtaagct tcttatttta taaaaaatat gtatgaaaga acagctgatt tttaatatta
     2521 caaatttagt aacggttgta tttgaagctt ttgtcatcca tatgttttta tcagactttt
     2581 taggccagaa agaagggtat ataaaagttg taagatatgc taaactgggt tttataatat
     2641 gtttaggatt ctgtaattta ataactttaa accctaaaat cacaatgcct ttaattttca
     2701 tccttatttt ttcaacatca tttttatata aaggaaattt aaagacacga ttgtttacta
     2761 ctgttttatt aagtatattc tttatactgt cagaaattgt agttacaagt atttttgttt
     2821 tatttgtaaa agaaggcttt gaaataatgc ttgaaaataa tagtataaga gtattggcga
     2881 caattttatc taaaattgtc tttttgttga cttgtaaaat tatttgtcta tttaaaaaag
     2941 atgtacattt agatatgccc ataaagtatt ggctcccttt atttttaata cctatattta
     3001 gtttatttct gtctgtttct atctttgatg taagtaaatt ttttagtctt gaaagtttaa
     3061 aatttctaag cttaatatct agtgttggta ttttatatat taattttatt gtcttttatt
     3121 tatttaaatt tattatagat aaaactaaac tatctatgaa atatgaacta cttgaaaaag
     3181 aaataattca taaggaagaa ttaagactta gtaatgagtg ttataaaatt attgttgaac
     3241 agactgattc tgtcgttttc gaatggaata tcaaagaaaa taaatcattt gtatcgcagg
     3301 cttggactga aaaatttgga tataacaatg catgtaagaa tatttttaaa gaaattaaag
     3361 ataaagactt agttcattct gaagataaag ctatatttga aggttttctt gaaagtatta
     3421 aaaaaaagaa tatgcacaat caagcagtat atcgtttaaa aaaatctaat ggtgaatata
     3481 tttggtgtag aacatcaatt acaagcattt ataatgatga aaatgaactt ttaagagttg
     3541 tcggagtaat tgtagatgta gatagtgata taaaaaaata tgaagagtta agaactagag
     3601 cagaaagtga ctcattgacc aatatataca ataaaggtac atttgaaaaa ttagtggaag
     3661 aaactatagt tatgaataca ggtgataaaa aagatgcatt attcattatt gatttggatg
     3721 attttaaaga aataaatgat aattttggtc atccatttgg tgattttgta ctaaaaacat
     3781 ttgctgataa gatacaaact agttttggta gtaaagactt agtcggaaga attggtggag
     3841 atgaatttgt tgtatatatg caagattatg ttacagaagt aaatttgcat aaaaaagcta
     3901 aagaattaaa tagagtactt tcggataatt acactgattt gagttttagt tttgatgctt
     3961 ctgtcagtat aggtattgca aggtatcctc aagatggaac ttcatttttt gaattgttta
     4021 aaaatgcaga tagagcactt tatagcataa aagcatcagg taaaaattca tattgtttat
     4081 ttgaagaaga actatatgta cagtgaattt gaatttagat tttatgtgag agtaagattt
     4141 atatatactt gtggctgtct gatttataaa atatgttact ttgtatattt ttatatgttt
     4201 cagtaaggtt aattaacata agcaaaggtg taagtaaatt tatattagtt taagtttact
     4261 tacaccttta taaaaataca aataatagat tcatttacat tcttaaatga ttaaagctct
     4321 ttaccttcta aacatgcata acatatcgtt ataattccag taaataagac taaaataccc
     4381 attcctaaga ttacagtttc attatctatt actccaaatg tttttaaagt aacttatttc
     4441 gctttgtatc ataataattt gtctcttttt acattttaac ttctgctgag atttgagtta
     4501 gatattcgtt agaatctcgc acacttatat catatagaag atattcttca taaacaaaat
     4561 ccccaatacg taaattattc tgattaaaaa aatttaataa cttttcataa gccttgtaaa
     4621 tagtttcata actcccttta tgataagcag ttacataaag tccctttggt ttgattgata
     4681 caggatattt cttgtactct ttattaactt ttgtaaataa atatgaatat gattcaaagt
     4741 ctttattaat tatatcttca cctttaatga ttgagccaat cgtaccataa tcactcaatt
     4801 catagttgtc cataaagtta atacattcat ataaaaaatt ttcttcctca tctcctaaaa
     4861 actgttcttt atatgtaaca ggtgttttga ccaagtactc ttcttcatgt tcttttaaag
     4921 taatagtatt cgcatctata ttacaggcat ttttagtgaa tgaaatagta gattccatta
     4981 gagcttgaat attatttagt ttttcaattt catttttaat atcaatggtt ttcttttcta
     5041 aaagctcaat tagtaaatta ggtgttcttt tgtctatata agctttaatt tcttttaaag
     5101 gcatttttac ttccctaagt gaagaaatta taccaaaaat gctaagttga tggtatgagt
     5161 agtatctata tccattttct ttctttaatt ctggtgagaa aagacctata tcatcataat
     5221 ggaaaagtgt ttttttattg attccacaaa tctttgcaaa ttctccagtt gtaaaataat
     5281 tttttgaaac ttgactcata attataattc ctccttgact atcctgttac agtatagttt
     5341 atcatagtat tttgtaaagg agtgtgatta tatgaaaaaa ttaattcatt ttttaaaacc
     5401 atatagagtt ttaattgttg tagttttgat atttacgttt ttacaaacac ttggaacttt
     5461 gtatatacca acacttacag caaatatagt aaacaatggt gtagtaaagg gagatataga
     5521 ttatattgta aaaacaggtt taatgatgat gatagtagct ggtataacag ctttatcagc
     5581 tgtgttagtt tgtaaagtat cagcaaatct ttcatctgga ttttgtagag atattagaga
     5641 agcagtattt ataaaatcac aagatttatc aataaatgat tttaataata taggtacagc
     5701 atcaatgatt actaggagta ctagtgatat tactttaata ggtcagtctg tgtttatgtt
     5761 tattcaatta gtattaccag caccaattat aactgtttct ggtctattct tagcatattc
     5821 tatagataaa gcaatgacaa ttattattgt tgtagtaatg tttttattca tgctttcagc
     5881 atttttagtt ggtaaaaagc ttataaagtt gtttaaaatg atgcaaataa aaatggacaa
     5941 tatgaatagg gtgttacgtg aggtagtaac tggagtaaga gttataagag cttttaatag
     6001 gagtcatttt gaaaaaaaga gatttgatag aactgcaata gattattctg aaacagcaat
     6061 ttcaataaat aaaatatttg cagtacttat gcctattgta atgttaataa tgaatttagg
     6121 tatagtaagt attatttggt ttggtgggat gagagtcagt aatggaaata tggaaatagg
     6181 tcatataatg gcacttgttg aatattgtat cttaatttta ttctatttaa tcatgggagt
     6241 aatggtattt atgtacatac ctagagcagg tgcatgtgca gatagagtaa atcagatatt
     6301 agatatagaa cctgaaattg tagatggaaa tggtcataaa gatactgtct cagagagagg
     6361 acatttagta tttaaaaatg taaccttttc atatgcacaa tcagaagaac ctgtgttaaa
     6421 taatataaca tttgaagcaa aatctggaga agtaactgca attataggaa gcactggctc
     6481 aggcaaatct actattgcaa atataatacc acgttttttt gaaattcaat ctggagaaat
     6541 atctataaat ggtcaggata ttaagaagat tcctcaaaaa gaacttagag ataagattgg
     6601 atttgtacca caaaaagctt ttttatttag tggaacaata gaagaaaata taaggtatgg
     6661 aaaagaagat gcaagtattg aagaagtaaa gcatgctgca agtatagctc aagctgatga
     6721 atttatttct gatatggaag acaagtatga ctcatttgta gcacaaggtg gaaataattt
     6781 gtcaggtggt caaaaacaga gaatatccat tgcaagagcg ttagttagaa agcctgaagt
     6841 atatgtcttt gatgatagtt tttctgcatt agattttaaa acagataaaa gacttagaaa
     6901 agcgttgaaa aatgaaatca aagattcatc agctataatt atagcacaac gtataagtac
     6961 aatcatggat gctaatcaga ttatagtttt aaatgatgga aaaattgtag gtattggaaa
     7021 acataaagat ttacttgaaa attgtgaagt ttataagcaa attgcggatt cacaacttag
     7081 taaagaagaa cttgcataaa atgaagaatt aatctgatat ttggaggtgt atgatatgag
     7141 tgaaaaaaga atagatgaac aagatataga agtacttagt tttgaagatg gcatgtcagc
     7201 agaaaaagct aaggatgcaa aaaaaactac taagagatta ttaaaatata tggcaaaaca
     7261 aaagtttaaa atattaatta tttttatttc agtattgata agttcagcgc ttacagtttt
     7321 agcacctatg gttatgggaa aagctattga ccaactattt aatggaataa aaactgcagt
     7381 ccaaacaggt acaaaattca gtgtgaattt tagtactatg ggggggatag tatcaatact
     7441 tctagggttg tacttaatta gtgcagtatt tatatatatt caacagtata ttatgtcagg
     7501 agttgcacaa aatctagttc ttagcatgag aaaagattta agtgataaac tcaataaact
     7561 tcctcttaag tactatgatt ctcataaaaa gggggaaaca cttagtatag taacaaatga
     7621 tttagaaaaa gtagcagata gtttacaaga aggattaatg caacttatta cagcagtagt
     7681 tacagtagtt ggttctattg taatgatgat aagtataagt gtaccactta caatagtatc
     7741 agctataaca ttacttgttt cattgggtat tactgttgta attgcaagaa aatcacaaga
     7801 aagattctct gaaaatcaaa aggcactagg agagttaaat agcaatatag aagagatatt
     7861 tactggtcaa atagtggtta aagcatttag taaagaaaag gatactataa gaaactttaa
     7921 aatattaaat caaaatcttt ataatgcaag tagaaaagca cagttttcgt catatgcaat
     7981 atctccaatt attagattta taaacctaat tggctatgtt attatagcag ttgtaggtgg
     8041 gatatttgca tcaactggag caatgactct tggtagtata caagcgttta ttcaatatgt
     8101 aaatcaagca tcagagccta ctacagaaat atcttatatt gtaaatatgc ttcaagcagc
     8161 aatagcatct gcagagagag tttttacagt tatggacgag gtggaagaga taaaggatga
     8221 agaatcttca aaggttatta gcatgcctaa aggtaaagta caatttgagc atgttaaatt
     8281 tggatatagt gatgatttta ttcttatgaa agatattaat atcaatctaa atgcaggaga
     8341 taagattgca attgttggac ctacaggagc tggaaagaca actcttgtaa atttacttat
     8401 gagattttat gagatacaag gtggaagaat tactattgat ggtgttaata taaaagattt
     8461 aaagcgtgga gaacttagaa ctatgtttgg tatggttctt caagatacat ggctgtttaa
     8521 tggaagtata aaagaaaata ttgcatatag taagtcagat gccacaatgg atgaaattgt
     8581 aagagcagca aaatcagcaa gagtagacca ttttataagg actttaccac aagggtatga
     8641 cactatattg actgaggatg cttctaatct atctcaaggt caaaagcaac ttctaacaat
     8701 tgccagggca atactttcag acccatcagt attgattctt gatgaagcaa catctagtgt
     8761 agatactaga acagaggtag aaattcaaaa ggcaatgaat aacttgatga agggaagaac
     8821 tagttttgtt attgcacata gattatcaac tattcgagat gctgatttaa tattagtaat
     8881 gaaagaagga actattattg aacaaggaag ccataaggaa cttattgaaa agaaaggatt
     8941 ctatgaagaa ctttataaca gtcaatttac aagtgaatat gatgaagatg ttgtatgatt
     9001 atcaagtttt aggcaactaa atgattgata aatattaagt taaatatagt aacatgtaag
     9061 gttatatgga ataagagtat ttctctattt catataacct tttttattta tcaaaataag
     9121 ataaaatttc ctttctaaat tagattgaag aatgtattta tcaatataaa agtaaaataa
     9181 tcattattat tagtgaagta aatattgttt gccacatata tgccataaag atgcacttat
     9241 tttacggaga tttaacaaag tagtgtaaag tatactctaa atataaaata aatcaaatag
     9301 agttaaataa attgagaggt gatataaata gtgaataaaa aattgaacta tagatttgaa
     9361 atgaagcaca aaataactga agcagatgtg ttagctttaa aatcaagact ctatcaaata
     9421 atgaagaaag atgaaaatgc atctaaagat ggaaaatatc ttattaggag tttatatttt
     9481 gatactcctg aagataaagc acttttggat aaattaaatg gcgttgcaat aagagaaaag
     9541 tttaggataa gattttataa taatgactgc tcttatataa ggctagagaa aaaaatcaaa
     9601 cattacaaca tgacatcaaa gctatcaact aatttaacca aaaaagaagt aataaatata
     9661 ctaaataatg atattgaatt tctaaaagag tcgacaaatt gtttattaag agaattttat
     9721 ttgaaattaa aatgtgaaag attagaggca aaaagcatag ttgattacaa tagagaagct
     9781 tttatttatc cagttggaaa tgtaagagtt acaatcgata gtgatattaa aacttctgta
     9841 aattcggtag atttatttaa taaagatttg ccaactgttt cggtaattga tgagaatatg
     9901 actgtattgg aggttaaata tgatgaattt ataccagatt ttataaagga tttaattcaa
     9961 attaataaga ctacctctac agcagtttca aagtatgctt caagtagatt atatatttaa
    10021 gaataaaggc tatgatttat ataaagatat acataaataa ggttaaaaaa caaataagtt
    10081 aaaaataaat aaaataaaaa ataaataagt taaaaatagg aaggatgatt ttaattatga
    10141 catttaatga tatttttaaa tcaagtttta tagaaaacgt atcaggtttt tcatttgtgg
    10201 acagtgcttt agcactaggt tcagcttttt tagtaggttt gtttatatat atggtctata
    10261 aaaagactta tatgggaatt atgtattctc gaccattcaa tgtgtcgctt gtagcactta
    10321 caatgcttac aacttttgta atattggcag ttacttcaaa tgtagtttta tccttaggta
    10381 tggttggtgc tttatctata gtaagattta gaacagcaat taaagaccca atggatttag
    10441 tatttctatt ttggtcattg ggttcaggta tagttttagg agcaggtttg attcccttag
    10501 ctgtcatggg ttcaataatt atgggattga ttctaatatt cttttcaaat aaaacaatat
    10561 ctgaaactcc atatatattg atggtaaatt gtaataatga agattcagaa gatatagcaa
    10621 ctgataaaat taaaaaagta tttactaaat atcaaataaa atcaaaaagt gttacaccag
    10681 aaaaaggaat agaattagtg tttgaagtaa gaatgaaaga tggagaaaca agtcttataa
    10741 atgacttgag tcaagtagat ggagttacta atgcagttct tgtaagttat aatggtgact
    10801 atgtagcata gagaggagta atcttttatg aaagataaaa aatttaccct tcttatctcg
    10861 attatgatta tatttttatg tgctgtagtt ggagtttata gtacatctag caacaaaagt
    10921 gttgatttat atagtgatgt atatattgaa aaatatttta acagagacaa ggttatggaa
    10981 gttaatatag agatagatga aagtgacttg aaggatatga atgaaaatgc tataaaagaa
    11041 gaatttaagg ttgcaaaagt aactgtagat ggagatacat atggaaacgt aggtataaga
    11101 actaaaggaa attcaagtct tatatctgta gcaaatagtg atagtgatag atacagctat
    11161 aagattaatt ttgataagta taatactagt caaagtatgg aagggcttac tcaattaaat
    11221 cttaataact gttactctga cccatcttat atgagagagt ttttaacata tagtatttgc
    11281 gaggaaatgg gattagcgac tccagaattt gcatatgcta aagtctctat aaatggcgaa
    11341 tatcatggtt tgtatttggc agtagaagga ttaaaagagt cttatcttga aaataatttt
    11401 ggtaatgtaa ctggagactt atataagtca gatgaaggaa gctcgttgca atataaagga
    11461 gatgacccag aaagttactc aaacttaatc gttgaaagtg ataaaaagac agctgattgg
    11521 tctaaaatca caaaactatt aaaatctttg gatacaggtg aagatattga aaaatatctt
    11581 gatgtagatt ctgtccttaa aaatatagca ataaatacag ctttattaaa ccttgatagc
    11641 tatcaaggga gttttgccca taactattat ttatatgagc aagatggagt attttctatg
    11701 ttaccatggg attttaatat gtcatttggt ggatttagtg gttttggtgg aggtagtcaa
    11761 tctatagcaa ttgatgaacc tacgacaggt aatttagaag acagacctct catatcctcg
    11821 ttattaaaaa atgagacata caaaacaaaa taccataaat atctggaaga gatagtaaca
    11881 aaatacctag attcagacta tttagagaat atgacaacaa aattgcatga catgatagca
    11941 tcatatgtaa aagaagaccc aacagcattt tatacttatg aagaatttga aaaaaatata
    12001 acatcttcaa ttgaagattc tagtgataat aagggatttg gtaataaagg gtttgacaac
    12061 aataactcta ataacagtga ttctaataat aattctaata gtgaaaataa gcgctctgga
    12121 aatcaaagtg atgaaaaaga agttaatgct gaattaacat caagcgtagt caaagctaat
    12181 acagataatg aaactaaaaa taaaactaca aatgatagtg aaagtaagaa taatacagat
    12241 aaagataaaa gtggaaatga taataatcaa aagctagaag gtcctatggg taaaggaggt
    12301 aagtcaatac caggggtttt ggaagttgca gaagatatga gtaaaactat aaaatctcaa
    12361 ttaagtggag aaacttcttc gacaaagcaa aactctggtg atgaaagttc aagtggaatt
    12421 aaaggtagtg aaaagtttga tgaggatatg agtggtatgc cagaaccacc tgagggaatg
    12481 gatggtaaaa tgccaccagg aatgggtaat atggataagg gagatatgaa tggtaaaaat
    12541 ggcaatatga atatggatag aaatcaagat aatccaatag aagttggagg ttttggtaat
    12601 agaggaggag gctctgtgag taaaacaaca acatacttca aattaatttt aggtggagct
    12661 tcaatgataa taatgtcgat tatgttagta ggtgtatcaa gggtaaagag aagaagattt
    12721 ataaagtcaa aataagatta aaattgaata taaataattt taatactgtt aagtctaaat
    12781 atttacttag cagtattttt tagaaaataa tgtgtttgat taataatata aggtaataaa
    12841 ttgataaaaa ttaaatacag catattaata atacataata aagtaataaa aaactgttta
    12901 agtataataa cttatattta gacagttttt ttcatttata aataatataa atgaaattga
    12961 aaacatttga aggatagaaa ttggttggat aaatgtataa attacttcac caaaaaataa
    13021 tgacaaaaac tgtaactaag ttgtaataaa atggtagtta tttagcataa aataatgtat
    13081 catagaagta tattataaaa ttaataaaat aagatttttg taagaaaact gctggctttt
    13141 gatatttaga gtgagtagtt aaattatgtt taggaggagt agaatttgtt gcaaaaagta
    13201 ttatggaaat ttttaaagct agtagtcggt ttatttatat gttcagttgg tatagtattg
    13261 acgataaatt gtaaccttgg attatctccc tgggatgtat ttcatcaagg actttcaaat
    13321 catataggaa taactatagg aactgcttct ataattgtag gctctatagt agttattgct
    13381 gatgtcgttc ttggagaaaa tgtaggatgg ggaactgttt ttaacatgct actaattgga
    13441 ttttttatgg atttactttt atatagtaat ttaattccag aggctgataa tttatttgta
    13501 ggaataatta tgttgatatt aggattggtt cttttgtctg ttggaatggt attttatatg
    13561 ggaagtggtt taggaagtgg acctagagat ggtttgatgg ttgcaattca aaagaaaaca
    13621 ggaaaatcac ttaaattaat aagaggtact atagaagttg gagcattaat agtaggattt
    13681 ttattaggtg gaaaagttgg tataggaact ataataagtg catttggatt ggggtatttc
    13741 acacaaatgg tatttggctt atttaaatta gattgtagta agattaaaca tagatttata
    13801 gtagatgata taaaatttat caaagtgtat atgagtggtg agaaaaaagc tgctgttcaa
    13861 aatatagtta agtaatttaa gaataaattc aacgaaatgt taagtatttt aaattaacat
    13921 tttgtttaga tagataaatt gaatattaaa agaatattaa aatatgagga ttgctagaac
    13981 actataaaat atagatttct agcaattttt tatttgttca atattttttt agtactagaa
    14041 gtcttattct ctatttaatg aatgaattaa tttataatta gagatactaa actttataga
    14101 tattaatata aacaccttta aactgtgtat tttccaaagc ctttaaaatg cttgtattta
    14161 ttctaaataa aaaaataaaa aaatgtcaaa aaaactcttg tattatatcc aaattttata
    14221 tataattacc tatgttgttt agaaatttta aactcaatgt ttagtaaaca aaatgtttag
    14281 taaaataaaa cagaaactag gaggtgcaac atggatatag gtgaaaagat aaaaagatta
    14341 agaacagaaa aacaactgac ccaggaagag ttagctaata gatgtgaatt gtcaaaggga
    14401 tttatttctc aattagaaaa taacttaaca tcaccatcca tagctaccct tatagatata
    14461 cttgaaatac taggtacaaa tttaagggaa ttttttaatg aaatagatga tgaaagaatc
    14521 agttttacaa aagaagatat gtttgaaact gaagatgaag atttaaaata taaattaaag
    14581 tggttaattc caaattcaca aaaaaatgaa atggagccaa taataataac attgtatcca
    14641 ggaggacagt ataaagaaga aaagccacat gaaggtgaag aatttggata tgtattagca
    14701 ggttcaatat atgtccatat aggcgaaaag aaaaataaag taaaaaaagg agagagtttt
    14761 tactttagac cgaaagcaaa tcactatata tcaaacgagg gcaaaactac agcaaaagtg
    14821 atttgggtaa gtacgccacc atcattttag aatagaggtg tttaataatt ggtagaaaat
    14881 ataatagaat taagggatat atctaaacat tatgaagaac ttactgtttt agataatttc
    14941 aatttagata taaagaaaaa tgagtttcta acattgcttg gaccaagtgg atgtggaaaa
    15001 actacaacat taaaaataat agcaggattt gagtatgctg atgatggtaa agttttattt
    15061 gaaggtaagg agataaataa tttgccacca tatgaaagac aagtaaacac agtatttcaa
    15121 aaatatgctt tatttccaca catggatgtt tatgaaaatg tagcttttgg attaaaaatt
    15181 aaaaagactc caaaagatgt aatagatgct aaagtaaaag aggttttgaa acttgttgca
    15241 ctagaaggtt ttgaaagacg acaagttgaa tcattgagtg gaggacaaca acaaagaatt
    15301 gcaatagcaa gagctctagt caatgaacca aaggtattat tactagatga accacttgga
    15361 gcacttgatt taaagttaag acaagagatg caaatagaat taaagagaat ccaaaaaaaa
    15421 ttaggaataa catttgtttt tgtaactcat gaccaagaag aggctcttac tatgtcagat
    15481 actattgttg taatgtataa gggcaaaatt caacaaatgg gaacaccaca agatatatac
    15541 aatgaaccta aaaattcatt tgtagctaag tttataggtg aaagtaacat atttgatgga
    15601 attatgatag aagattataa agttaatttc tgtaatagag attttgagtg tgttgataaa
    15661 ggattcgaaa aaaacgaaaa tatagaagta gtaataagac cagaagatat aaaaatggta
    15721 aagccagaag aaggaatgtt aaaggggaaa gttacatcta cagtgtttaa aggagtgcat
    15781 tacgagatag aattgaatga aaatggaaga ttgtggatac ttcataatac taaaaatgct
    15841 gaggttggga ctgaactagg catggatata tatccagaag atatccatat aatgagaaaa
    15901 gagaataact aagatgaaaa gaaaatcttt tttagcatat ccatatgtag tttggagtgc
    15961 gatatttgta ataattcctt taatattagt agtatttttt agttttacaa aagaatcagg
    16021 tggaggttat gcctttacac ttgagaatta taaagaagta attgacccta tatatatgaa
    16081 agtttttggg cgttctattc ttttagcagg aggagctaca ttaatatgcc ttatagttgg
    16141 ttatccagtg gcatatataa tttcaaaggc tcgtgtaagt agaagaggtt cattaatatt
    16201 attgtttatt cttcctatgt ggatgaactt tcttttaaga acatatgcat gggttgctat
    16261 attaggaaaa aatggacttt taaatacatt tttaggttgg tttggaatac aaccattagc
    16321 aattttgtat actaattttg ctatactttt aggtatggtg tataactttt taccatttat
    16381 ggttcttcca atatatacag cactatcaaa gatggataat gatttgataa acgctgctca
    16441 tgacttgggt gcaaataata tgacagtttt tagaaaaatt atatttcctt tgagtttgcc
    16501 aggtgtaatg tctggaataa ctatggtatt tatgccagca gtcactactt ttgcaatatc
    16561 aagactgtta ggtggaggta aaataatgct ggttggagat ttgattgaac aacagtttac
    16621 agttgttgga gattggaact ttggttcagc tatatcgata tttatgatga tagtaatatt
    16681 aatatctatg tctataatgt caaaatttgg agatgaatct gacaaagaag gcggtgggtt
    16741 actattttaa aattaaagaa aataacttct aatatatatt tagcattagt attcttattt
    16801 ttgtatgcac ccatatttgc tttggtacta ttttctttca atgattcaaa atcaatggca
    16861 agatggaatg gatttacatg gaagtggtat ggtcaattac ttcaaaatga gagtataatg
    16921 agtgctttat attatactat tgtaatagct atattagcgt ctgtaatatc tacaatagta
    16981 ggaactataa gtgctatagg aatacataag atgagaggta agagtaagaa attaatacta
    17041 aatgtaaact atcttcctat tttaaataca gagatagtta cagcagtagc tcttatgagt
    17101 ctgtttgtat ttgtaaaaat ggaatttgga tttacaacaa tgctattggc acatataatg
    17161 ttttgtttgc catacgttat actttcagtt ttgccaaaaa tgaagcaatt acctgataat
    17221 atagaagatg cagctatgga cttgggagca acacctatat acgctcttag aaaagtaatt
    17281 ttaccacaaa taaaaccagg tatagtttct ggatttttaa ttgcatttac tatgtcaata
    17341 gatgacttta taataagttt ctttaatgct ggaaatggtg taagcaatct ttctatagaa
    17401 atctatggaa tggctagaag aggtataaaa cctgaaataa atgcattatc aacaataatg
    17461 tttgcagtgg ttctaggttt acttttacta gctaataaaa aagaatctat agtaaggggg
    17521 attaaataat gattaagttt aaaaaagcaa tatcccttat agctgtaggt atgatgacag
    17581 ttactttatt tacaggatgt aataaagcaa ctgattctac aaaagtttta aatgtatata
    17641 atgttggaga ttatatagat gagtctctta tagataagtt tgaaaaagaa acaggaatag
    17701 atgttcaata ttcaacttat gatactaatg aaatgatgta tcaaaaagta aagagtggta
    17761 gtacacacta tgatttagta tttccttcag actatatggt agagaaaatg aaaaatgaag
    17821 gtcttttgga aaaattagat tttaagaata tacctaatat gaaatattta gataagagtt
    17881 ttttaaatcc tgtatacgac aaaactaatg aatatagtgt tgcatatatg tggggaactc
    17941 ttggaatatt gtacaataag aaagaagtta aagaccctat ggatagctgg aatatacttt
    18001 ggaatccaaa gtataaggga aatataatga tgtttgactc tgttagagat actataggga
    18061 taactcttaa aaaattagga tatagcatga atagtgtaaa tccaaaagaa ataaatgaag
    18121 ctaaaaatct tttaatgaaa caaaaggatt tagtattggc ttatgtaaat gatgaaggta
    18181 aggatagatt gcttggtgga gaagtggcaa tgggtatgct gtattctgga gatgcagtta
    18241 cactaatgga gcaaaatcct gaccttgatt atgctatacc taaagaggga actaataaat
    18301 gggtagatgc tatgtgtgta cctacaacag cacaaaataa gaaagaagct gagctgttta
    18361 taaacttttt attagaccca gagaatgcaa aagtaaatgc tgagtacatt gggtattcaa
    18421 cacctaatac aggagcacta aaattattag acccagaaat aactcagaat cctgttgcat
    18481 atccatctaa agaagtactt gataaatgtg aaacttttga agatattggt gaaaatataa
    18541 aattatatga caaggcttgg atagagttaa aatcaaaata gggtcttgtt gttttaagta
    18601 aatttactta ataaatactt aataaaactg ttctttttta gaaaatttat tttctaatta
    18661 ttgaacagtt ttattttttg tttaagttat attcatatgc aatttaaata gtttattaat
    18721 aaatgaaaac ttatttgtat aatgacttta ataataatta gaaaaaatgg gcaacttaat
    18781 agaataattg gaggtgtaaa tttataaaaa atgaatagga taaataaaaa aatagattta
    18841 ttaatattgg ctttgctcgt ttttatattt ttaattgttg gaatagtaat actatcgatt
    18901 aggacagaaa ataaattgaa tgtgttcata atgttagcaa taattttttt tataataatg
    18961 ttaacttatt tatcaaattc agtagtgggg ttgataacaa gttcaataat tatatttatg
    19021 tatacatcct atattttata taacaatatt actcacaata tggatgttga atttatatca
    19081 tatatgtgga taatagcaac accagtatct tcaattataa tgggtaatct gaacaaaagc
    19141 ataaatgaat tgcaaaatac aaataaaaaa ctatctgaac agtataagga attagtgact
    19201 attgatagtg aaactggact tagaaattta aagatttttt ataatgatgt aaatatggag
    19261 ataagtaaat ctattagaca taatactgat ttttctctta tgatagtaaa attaccatac
    19321 tacggaaacc tacagactat atttggtgaa aacaaaacta ataaaatagt aaaatatatt
    19381 ggttcaaata ttatagaatg tacaagaaat gaagatataa tttatagttt acaaaaagat
    19441 atgataggga tactaatgcc aaatacatct ttggaaggtt caaaagttgt taaagacaga
    19501 ataaagaaaa ggataaaaga attaaattta gatttaaaca ataggggaaa atatgtaaat
    19561 atagatgtca aaattgcatt tttacaatat aagagtagtt ttggagatag tataaatttt
    19621 aagaatatag ttgaagagga acttcaatat gatgtttaaa atatttaata aaagtctatt
    19681 aaaattagta gttgtattat caatagtact gacaatatca aattttagct tatcttcata
    19741 tgcatcagaa attacttcca gcacagataa ggtattaatc atatatgatt ctaagaaaga
    19801 aacagcttat aatagagata ttttaaatat tatgaggact ctattgggaa gatttagttc
    19861 agatatagag ctattaaaat tgagtaacta tgatggtgag ataaataaaa attattacag
    19921 tcatatattt attttaggaa tcaatgaaaa ctcatataat aatgataaaa acactaaaaa
    19981 cttgattagt tccttaaatt catataaagg aactatatgt tggttaggat atggtataga
    20041 aaatcttttg gaacataaaa aatacaattt agactatgta ggaaaaacaa acaatatagt
    20101 tagtgtaaat tatagaggta aatcatataa cttggatgaa cattatgttt ttaacattgt
    20161 agaatcaaaa gatacaagta ataaagtaat aggaagtatt aatgatacct taaataagta
    20221 tccatatata ataaatgata aaaatctttt ttatgtatca aaattagatt tagatggagt
    20281 tttattttat atattttgcg attcacttaa tgatattttt aatattaaga cgtttgataa
    20341 aggacgtata tttgtaagaa tagaagatgt acatgctttt agagaaccaa aaaatcttgt
    20401 agagatagcg gattatttaa gtagtaagaa tataccattt acgattgcat taataccagc
    20461 atatgtaaat cctaaaaatc ataaggtaat aactttatca gaaagccctg aaattgtcaa
    20521 agcaataaaa tatatgcaag ataaaggtgg aactgtgata ttgcatggat atactcatca
    20581 atacaagaaa gaagaagttt ctggagaagg gtatgaattt tgggatggta aaaaagatga
    20641 acccttaaaa gaaaatatga agatatttgt caaagataga gttttgaatg gtcttagagt
    20701 atgtatagag aatggaattt atcctcttgc atttgaagcg ccacattatg caatggaatc
    20761 agagggatat aaagaattaa aaaaatattt ttctacttat atggggcaac atcaaaataa
    20821 tgataaaaaa tttagcacaa atacatatcc ttatattatt agggatacag aggagtttaa
    20881 tatatttata cccgaaaatt taggatatat agacccagaa gataagttta cttttcaaaa
    20941 tataaaagaa aatttaaata aattatccat tgtaagaggt tttagtggag ggttcttctt
    21001 tcattcatat ttaaacatag agtatctaaa aaacacaata gaatatttag aaaaacagaa
    21061 tatagaattt atgaatttaa gagattttaa taattgggta aaagttgatg aaatacaaat
    21121 aagaaataat ggagatgaaa ttatagtcaa ctatgataag gacttagatg aaataactaa
    21181 aagtgatact agatttaaat ccatatccaa tattagtaaa gttttaatat tcatagtatc
    21241 tataagtgtt ttaatatttg tgattatatt tatatatttt aagagaatag ataaaaagaa
    21301 atttttaaaa taaatgattt tatattagaa tcaatatttt aaaatatttt aggagaaatt
    21361 tatgagagat ttaaatgtat cagagtattt gttcgtattt agtctgttta gtatatggtc
    21421 tttattgctt ataaatatta ttttagctat gggtggatat atattttatt ttaaaaactt
    21481 tgataaagaa ataaaagaaa ttgatgaata tcctatgata tcaatacttg ttcctgctca
    21541 taatgaagct aaagtgatag gtagaactgt ggagtctttg ctattactca attatcctaa
    21601 gagtaagatg gaacttattg ttataaatga caattcttct gataatagta aagaaatttt
    21661 agaaaatata aaggataggt acaacaatta taatttcact ataataaata cagatagttt
    21721 aacaggtgga aaaggaaagt ctaatgcatt aaatattgga tatacgattt caaaaggaga
    21781 ttttatagct gtttatgatg cagataacac accagataaa aatgcactta gatatctagt
    21841 acaaactata gtaatgaatg atgaattagg tgctgttatt gggaaattta ggactcgaaa
    21901 taaaaataaa aacctattaa ccaaatttat aaacatagaa acattaagct ttcaatggat
    21961 gtcacaagct ggtagatggc aactttttaa tctatgtaca ataccaggaa ctaactttat
    22021 attgaggaga agtatcatag aagaaattgg aggatgggat agtaaagcaa ttgcagaaga
    22081 tacagaaata agttttagga tatataaatt gggatacaag ataaagcttg taccacaatc
    22141 tataacatgg gaacaagagc cagaaactgt taaggtgtgg ataaagcaac gtactagatg
    22201 ggcaaaaggg aatatatatg tattaatgaa atatataaaa aatatattta aacagggaag
    22261 aaataagatt gtgtttgata tagcttactt tttttctgta tattttctat ttttaacatc
    22321 tgtaattata tccgatattt tatttgtttt aagtatatct aaactagtag aaatttctat
    22381 tccaattaac ttttttctaa tatggatact atcttatctt ttatttatta tagaagtgag
    22441 tataagcctt actatagaaa aaggtgaagc aacaattgaa aatatattca tagtggcaat
    22501 aatgtatttt acatactctc aactatggct gtttgtagct ataaaaggaa tgatagagta
    22561 tctaaaagat ataattttta aaagagaggt taaatggtat aaaacagaaa gattttgatt
    22621 catgtacagg aggaattaac atgaaaaaat ttattatatc aattatatct ttagtattgt
    22681 ttttttcaaa tatatcactt atatacaaag taaatgctga tgagactaaa gttaaaaatt
    22741 ataagtttga aagagatata actattgatg gtgttatagg aagtaatagt actttttttg
    22801 aagtaaataa aaattgggat attgaagaag ttttattgca tcttaatttt agtaagagcc
    22861 aaatattaaa tggagatgtt tcaagtttaa ctgtattgat aaataatgta ccaataaaat
    22921 ctattaaatt aaatgctaag acaaattata aaaatacatt agaagtatta gttccaaaag
    22981 attatataat tcaaggatat aatgaaataa aaataaaaac atacaaaaca atatctgata
    23041 aaatttgcca agatgattcc aatactggga actggatggt tattcacaaa gaatcatata
    23101 tatcaattag atataagcag aaaaaggtgg aaaactcaat aaatgaatat ccgtatccat
    23161 atgcagaaat tgaaaataat cataaattag atacaactat agtagtacca gataatatga
    23221 ctagaggaga gactacagca gtgtttaatt tggcatcagc atttggaaaa ataacaaaaa
    23281 atgatgattt aaagttagat gttaaattat attcagaaat gaaaaattgg tcagatgata
    23341 atattatata tattggtaaa cctgaaaata cagcagaaga aattttggat attttaagta
    23401 taaaggaaca aaccctatta tcttcaaact gtataatcaa gcaagtagat tcaccataca
    23461 ataagaataa gaagatgatg gtggtaattg gtagtaatga agatgattta ataaaggcat
    23521 caaatttatt aatagaaaat agactttcta accaagtttt atcatcatca gtacttgtaa
    23581 acaaagagac taatataaaa attaataggg aacaaaaatt aaatttaggt catttaacat
    23641 taaaagactt aggatattct gactttttgt tggaaggagc atttaatcaa caagctttgt
    23701 ttgatgtcaa aataccaacg ggtaaggtat tagatgatgg ttcaaagatt atcttaaatt
    23761 taagatattc tgacaattta gattttgaaa aatctttagt tacagtgtct ataaatgatg
    23821 ttattgtggg aagtaaaaaa ttggataggt cacattctaa taatgataag ttagaattaa
    23881 aaatacctaa agatatagat aataaaaact attatcaagt taaattgaca tttaatttga
    23941 gtataaaaaa ttcgaattgt gttactagag agagtaataa tccttgggca tatgtatcaa
    24001 ataattctta tttggcgtta tctatgaaag aaaatgaaac cttatctttt gaaaattatc
    24061 cgtatccatt tgttagagat gatgaattta atgatttaac tgtgataatg ccagattatt
    24121 cagggtcaca agctatgact tggatgttta gattaggtgt tactttaggt gcgaatataa
    24181 attcacataa tggaaatata aatgtaatta gaggaaaaga atttagtgac aagtataagg
    24241 atactaatat tgtagttttt ggagtgccac ataataattc tgttataaag atgttaaata
    24301 ataatttaaa tataaaattc gataaaaatt actccaattt tatatcaaat gataagataa
    24361 gctttattga tgattatgga aaaaatattt ctaccattca attaataaaa tcaccatata
    24421 ataatcaaaa aaatatcatg gtaattagtt caatgaatga aaaaaactta tatttaggaa
    24481 tggattattt gttgaataaa agtaaagtta atgatttaaa aggagataca ttgataattg
    24541 atgaatacgg agaggtagaa gatttagctt ataatttaaa atctaagaaa gaagtaaaag
    24601 attcaagttg gaatatgagt ataaataaga caactaaagt atttttaatg atttctttta
    24661 ttactataat tgta
//
LOCUS       DBGJFU010000052        24144 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.052, whole genome shotgun sequence.
ACCESSION   DBGJFU010000052 DBGJFU010000000
VERSION     DBGJFU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.052"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..197)
                     /locus_tag="ACOIAS_001980"
     CDS             complement(<1..197)
                     /locus_tag="ACOIAS_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2701692.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNGNKFILISYF"
     gene            381..725
                     /locus_tag="ACOIAS_001981"
     CDS             381..725
                     /locus_tag="ACOIAS_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGZ2701693.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPVRFDVIEVYL
                     LEKRINHIMNAF"
     gene            756..2357
                     /locus_tag="ACOIAS_001982"
     CDS             756..2357
                     /locus_tag="ACOIAS_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGZ2701694.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2376..3476
                     /gene="dprA"
                     /locus_tag="ACOIAS_001983"
     CDS             2376..3476
                     /gene="dprA"
                     /locus_tag="ACOIAS_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGZ2701695.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3509..5596
                     /gene="topA"
                     /locus_tag="ACOIAS_001984"
     CDS             3509..5596
                     /gene="topA"
                     /locus_tag="ACOIAS_001984"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGZ2701696.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5761..6546
                     /gene="codY"
                     /locus_tag="ACOIAS_001985"
     CDS             5761..6546
                     /gene="codY"
                     /locus_tag="ACOIAS_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HGZ2701697.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6653..7918
                     /locus_tag="ACOIAS_001986"
     CDS             6653..7918
                     /locus_tag="ACOIAS_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HGZ2701698.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDSHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7949..8719
                     /locus_tag="ACOIAS_001987"
     CDS             7949..8719
                     /locus_tag="ACOIAS_001987"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2701699.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMIFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8828..9274
                     /locus_tag="ACOIAS_001988"
     CDS             8828..9274
                     /locus_tag="ACOIAS_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGZ2701700.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9292..10485
                     /gene="nifS"
                     /locus_tag="ACOIAS_001989"
     CDS             9292..10485
                     /gene="nifS"
                     /locus_tag="ACOIAS_001989"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HGZ2701701.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLDNRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10539..10979
                     /gene="nifU"
                     /locus_tag="ACOIAS_001990"
     CDS             10539..10979
                     /gene="nifU"
                     /locus_tag="ACOIAS_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HGZ2701702.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11268..12347
                     /gene="mnmA"
                     /locus_tag="ACOIAS_001991"
     CDS             11268..12347
                     /gene="mnmA"
                     /locus_tag="ACOIAS_001991"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGZ2701703.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12490..12728
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12796..15435
                     /gene="alaS"
                     /locus_tag="ACOIAS_001992"
     CDS             12796..15435
                     /gene="alaS"
                     /locus_tag="ACOIAS_001992"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGZ2701704.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15460..15717
                     /locus_tag="ACOIAS_001993"
     CDS             15460..15717
                     /locus_tag="ACOIAS_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HGZ2701705.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15727..17898
                     /locus_tag="ACOIAS_001994"
     CDS             15727..17898
                     /locus_tag="ACOIAS_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701706.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSCASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18104..18529
                     /gene="ruvX"
                     /locus_tag="ACOIAS_001995"
     CDS             18104..18529
                     /gene="ruvX"
                     /locus_tag="ACOIAS_001995"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGZ2701707.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18607..18894
                     /locus_tag="ACOIAS_001996"
     CDS             18607..18894
                     /locus_tag="ACOIAS_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HGZ2701708.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19176..19634
                     /locus_tag="ACOIAS_001997"
     CDS             19176..19634
                     /locus_tag="ACOIAS_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HGZ2701709.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19751..20488)
                     /locus_tag="ACOIAS_001998"
     CDS             complement(19751..20488)
                     /locus_tag="ACOIAS_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGZ2701710.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20705..22381
                     /locus_tag="ACOIAS_001999"
     CDS             20705..22381
                     /locus_tag="ACOIAS_001999"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGZ2701711.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22589..22789
                     /locus_tag="ACOIAS_002000"
     CDS             22589..22789
                     /locus_tag="ACOIAS_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1290 family small acid-soluble spore protein"
                     /protein_id="HGZ2701712.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22843..24006
                     /locus_tag="ACOIAS_002001"
     CDS             22843..24006
                     /locus_tag="ACOIAS_002001"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2701713.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLT
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
BASE COUNT         9876 a         2491 c         4470 g         7307 t
ORIGIN      
        1 aaaaagtaag aaataagtat aaatttattc ccatttgata aagaaaaaaa tctaacttga
       61 ttatttatta taattagaaa taatattatc atagttatga ccggatacaa tgtattttca
      121 acatttttca aatagcttac aactaatata aataaagata aatatcgtat aaaagcaact
      181 aatttttctc tgttcattaa aggcactcct ccaaaaaaat aaacttttac tatgaaaact
      241 agaataatat cataaaagaa taaagtcaat aaaagtattt attaatattg atggaaataa
      301 aatcaatcca aaaacttgaa tagtaaaatt gtatactgac aataatttaa aagtgaaaat
      361 ttaatgtatt ggagtgaata atgaataata aagaaaaagg tgattttgga gaaaaagtag
      421 cagttaatta tttgctatca aaaggagcaa agatactaga aaaaaattac agattaaaga
      481 taggggaaat agatataata gcaaaaatgg aagatgaaat tatatttgtg gaggtgaaat
      541 caaggtctaa cataaagttt ggctaccctt gtgaatctgt aagcttcaaa aaaagaaaaa
      601 agataattgg agttgcaaat tattatatta taaaaaataa cttaaataac actcccgtaa
      661 gatttgatgt tatagaggta tatttattag aaaaaagaat aaatcacata atgaatgctt
      721 tttaaaaaag ataatgtttt tggaaaggat taaatatgtt aagtattatt aattcaagca
      781 acttagtagg aatcgacagc tttttagtta aggttgaagt agatgtaagc aatggaatac
      841 ctagttttaa tattgttggt cttccaggca aagaaattaa agaagcaaga gaaagagtaa
      901 agtcagcaat tcttaatagt ggatataaat ttccaagcac gagaatagtt gtaaatttat
      961 caccagcaga tataaaaaaa gaaggagctt ttttagattt atctataagt ataggccttt
     1021 taagagagtt aataaaaaaa gatgaaaatt acataagaga aagtatgttt ataggagaat
     1081 tatcattaga tgggaaaata agaaaagtaa gaggtatatt gcccataatt atgggtgcaa
     1141 aaactcagaa cataaaacgt atttttatac caattgagaa cataaaagaa agtctatttg
     1201 tagatgaaat tgatataatt cctattaaaa gtttaaaaga atgtgtcgat tttttaaacg
     1261 aagagataaa agtcgataaa gtaaggataa tgtctttttt ggatgataag tcaagaaaag
     1321 agaacgaaga attagaaaaa gataatagtt atatagattg caaatatact aagattaata
     1381 atgaagaaag taaatatgat gaagacttta aagatgtaaa aggaaattat tttgtaaaga
     1441 ggagtgcaga aattgctgct gctggaaatc ataatatgtt tatgattgga cctccaggct
     1501 caggaaaaac tatgattgca aaaagagtaa ggacaatttt accagatata agtgtagaag
     1561 aaatgataga agtaagtaag gtctatagta tattgggtat gataaatgaa actaaaggaa
     1621 taattgataa aagacctttt agagcacctc atcatacaac tacaaagcaa tctttaattg
     1681 gaggaggaat ggatgcaaga cctggagaaa ttgctttagc acatagaggt atcttatttt
     1741 tagatgagat agccgaattt gatagaaaaa tacttgagac tttaagacaa cctatagaag
     1801 atggatatgt aaatatatcc agagtaaagt acagtgcaaa atatccttgt agagtactgt
     1861 tagttgcagc tatgaatcca tgtccatgtg gttattatat gtcagagaca gagtgtagat
     1921 gtagaagtaa cgaaattgat agatacataa ataaaatatc aggtccattg ttagataggt
     1981 ttgatatttt tgtagaagtt aattctataa aatattcaga ttttaattct ttaaaacaag
     2041 aagaaagctc tcaaaaaata aagagaagag tagaaaatgc tagaaaaata cagataaata
     2101 gatttaagaa ggataatata aaaaataata gtgaaattaa agcttacaat ttatttaaat
     2161 attgtaaact ggaaaaagaa gcttcaaaaa cggctgagat gatatttaat aaatacaacc
     2221 taagcagtag aagttataca aaacttttaa aaatggcaag aacaatagca gatttagaag
     2281 aaagagactt gataaattca cagtgtatta tagaagcttt ttcatttaga aaggcttatt
     2341 atagttattt taaatagttt tttgaggtgg attttatgga aaaaagagat acttatttat
     2401 ggttaaaatc aattggtgga ataacaacaa aaacaattga aataatagaa aatgaaatag
     2461 taaatattga agatatattt gatttttctg aaaaagaaat atataattta aaaaatataa
     2521 gcttgaatat tagaaaaaat atagtaaaat atagaggtca cgcttattta gaaaatataa
     2581 aagaactact ttacaaaaaa gcgattaaat atatttgtaa atatgataaa gaatatcctg
     2641 aaaatttaaa gaatatatac aacgctccaa agttattatt ctacaaaggt gacataggct
     2701 tagttaataa taattttaat atagctatag taggttcaag aaaacctaca gcctatggaa
     2761 ttaattgtgc aaaaacaata agttgtcaac tatcccaata tggagttaat atagtaagtg
     2821 gattagctat aggaatagat gcatactcgc acataggttg tatgagtgga aaatctaaaa
     2881 ctatagcagt acttggttca ggagtagaca atcctcttcc aaaacaaaat ttacatttat
     2941 caaataaaat attagaaaat gggggattat tattatcaga gtataatatt aactcgacag
     3001 tagccccgta ccatttttct aatagaaata gaattatcag tggtttaagt gatggagtag
     3061 ttgttgtaga agcagcaata aaaagtggtg cactaatcac agtggatttt gctcttgaac
     3121 atggaaaaaa tgtttttgca atacctggta atataaattc tcaaatgagt agaggttgtc
     3181 ataaaattat aaaagaaggt gcaaaattaa ttgaaaatat agatgatatt ttaaatgaat
     3241 ataacatatt taatattatt gataaaaaaa taaatcaaaa atatgataat ataagtctaa
     3301 atgccaagtc taagcaaata attgaagcta taaaaagaga aggaaacttg catatagata
     3361 gtatttgtga ttatactggt atagaaataa agtatgtaaa ttcaataatc aatgaattag
     3421 tactaaatga gttagtagta gaaatgaata ataaaacgta cagtttaaat gtataaatta
     3481 agattaggta gtgggggtgt atggttttat ggccaaaaca ttagtcatcg tagaatcgcc
     3541 tgcaaaagct aaaactatag aaaaattttt agggaaaagt cattacacag ttaaagcatc
     3601 agttggtcat gtcagagatt tacctaaaag taaattaggt gtagatatag aaaataattt
     3661 tgaacctcaa tatataaata taagaggaaa aggcgatgta ataaaagaat taaaaaaaga
     3721 agctaaaaaa tctaaacaag tatatctagc aactgaccct gatagagaag gtgaagctat
     3781 atcatggcat ttagctcata tactaaattt agatgaatct gaggattgta gaatagagtt
     3841 taatgagata actaaggatg caattaaaaa agcaattaaa catcctagaa atattgatat
     3901 aaatttggtt gatgcacaac aggcaagaag agtgctagac agacttttag gttatcaaat
     3961 aagtcctata ttatggcaaa aggttagaaa aggtcttagt gcaggtagag tgcaatctgt
     4021 tacaacaaag ttaatttgtg acagagaaaa agaaataaag gcatttattc caaaagaata
     4081 ctggacaata gatgttgagg ctaagacagt aaaagacaaa gatataactt tgaaatttta
     4141 tggtaaaaat gatgaaaaga ttgaattaga aaatgaagaa gctgtaaatg aaatactaaa
     4201 agaaatggaa ggtaaagatt taaaggttga gaagatagaa tctaaatcaa gacgtaaatc
     4261 agctccaaaa ccatttacaa caagtatgct tcaacaagaa ggagcaaata agttattttt
     4321 tactactaaa aaaacaatga tgatagcaca agaattatat gaaggtatcg acatagaagg
     4381 tgaaggtact gttggtctta tatcatatat aagaactgac tctaaaagaa tatctgatga
     4441 agcaaaagaa aaagctaagg gctatatcgt aaacaattta ggagaaaact actataaaaa
     4501 cactggtgat aaaaaagaaa aaaaggatac taaaaaggtt caagatgccc atgaggcaat
     4561 aagaccaacc tcagttgata gaactcctga gagtataaaa gcctcattga gcaaagacca
     4621 gtacaagtta tacaatttaa tttggagaag atttgtagct agtcaaatgg aagattcagt
     4681 gtttgatata ctaaatgtag aatgtaaaat aggcgatatt gtatttaagg caactggttc
     4741 aaaaatgaaa tttgatggtt ataccaaggt atataatttt tcagaaagag aagacaaaat
     4801 tttaccaagt atagaaaaag gagatatatt aaatatagaa aatatattgc cagaccaaca
     4861 ttttacacaa ccaccagcaa gatatacaga agctagttta gtaaaaacac tagaagaact
     4921 aggtataggt agaccaagta cgtatgctcc aacaatagct actatactga atagagagta
     4981 tgttgaaaag caaggaacaa gtctatgtcc aacagaactt ggaataatag ttactgatat
     5041 tttggaaaat aatttccata aatttataga tgtagatttt actgctgata tggaaagtaa
     5101 acttgatgtt atagaagaag gcaatacaga ttggaaaaag attgtatcag aatcatatgc
     5161 accacttaaa gaggctattg aagaagctct tgaaaatata gaaaaagtaa atatggatga
     5221 agaaactgat gagatttgcg agaactgtgg gtctaatatg gttatcaaat atggtagatt
     5281 tggaaagttt atggcttgta aaaactatcc cgattgtaaa acaactagac caattatgaa
     5341 taaaattggt gttaaatgtc caaaatgtga agaaggagat attatactta gaaaatctaa
     5401 aagaggtaaa gctttttatg ggtgttctaa ttatccaaac tgtgactttg tagcttggaa
     5461 taagccaaca ggagagtttt gtgagaagtg tggttcatat atggttgaaa aagtgactaa
     5521 atctgaaaca aaaacagtgt gttctaataa agaatgtaat aaagagacaa aagagcataa
     5581 tgacggaaaa gagtgaaaat tgaaaaaaat cacataattc tcttgaaaaa ggttccaatc
     5641 tatgataatc tttcaagtgt aaacaactaa tagaaaaata aattttcatt aaatgtgaaa
     5701 ataatattag aaaatataaa aggttattaa aaggcctaaa tttcatgagg agatgattaa
     5761 atggcaagtg aagtgttaca aaaaacaagg aaaataaata aaacattaca aacaagtggt
     5821 ggaagcagtg tctcttttga tttactggcc ggagcattgg gcgacgtttt aagttctaat
     5881 gtttatgtag taagtgcaaa aggtaaagta ctaggtcttc atttaaatga tgttcaagac
     5941 agttcagtta tagaagatga gtatactaag caaaagaaat tttcagatga atatactcaa
     6001 aatgtgttaa aaattgatga aacattagaa aatttaaatg gtgagaagat attagaaatc
     6061 tttcctgaag aacatggaag attacaaaaa tatactacag tagttccaat attaggaagc
     6121 ggtcaaagat taggaacatt ggtactttca agatattcaa attcattcaa tgatgatgat
     6181 ttagtaatag ctgaatacag tgcaactgtt gttggtcttg aaatattaag agcaataggt
     6241 gaagaattgg aagaagaaat gagaaagaaa gctgtagttc aaatggcaat aggcactctg
     6301 tcctactccg agcttgaagc agttgaacat atttttgctg aattggatgg aaaagaaggt
     6361 ctacttgtag caagtaagat agctgataga gttggtataa ctaggtctgt aatagtaaat
     6421 gcacttagaa aatttgagag tgcaggtgtg atagaatcaa gatcattagg tatgaaaggt
     6481 actcatataa gaatacttaa tgacaaactt acagatgaat taaaaaaatt aaaaaacaat
     6541 caataatcta tattttatag gtttagatta gaaaaggtat ctatttcaaa tagatacctt
     6601 ttttagacga attattaagg taatgataag tatattaaaa aggagtgaga aaatgccttt
     6661 agcagataaa ataagaccaa aaacaatgaa tgatgtaata ggtcaaagtc atataatagg
     6721 taatggaaaa atattgtcta agatattaca atctaatttt ttaccaaata tgatattttt
     6781 tggtcctcct ggtgttggta aaactacagt agcggaaata atagcagaaa gaagcaataa
     6841 aagcttctat aaaataaatg ctactaattc atctttagaa gatataaaaa aggtgatatc
     6901 agagcttgga agtataaata atgtaaatgg agtcctatta tatatagatg aaatacaaag
     6961 ttttaataaa aaacagcaac aatcaatatt agaatttatg gaaaatggga gtataacttt
     7021 gattgctagc actacagaaa atccatatca ttatgtatat aaagctcttt taagtaggtc
     7081 tactgtattt gaatttaaac ctttagaaaa acttgatgta gaaaaaggac tgaaaagagc
     7141 tgttgaagta ttaaatgaag atagctatat ggatattgag tgtaataata atgctattga
     7201 agatatagca attctttctg atggagatat gagaagagcc ttaaatattt tagaagtggt
     7261 agtctattca actaaaccaa ataaggataa tataacttat atagattcag atgtaataag
     7321 ggcatctaca tttaataaaa ttataaacta tgacaaaaat ggtgatagtc attatgatat
     7381 tttaagtgca tttcaaaaat ctataagagg aagtgaccca caggcatcta tacactatct
     7441 agcaagactt ataaaaggtg gggatttgat aagtatatgt aggagacttt tagtaatagc
     7501 tagtgaagat ataggtttag cttatccaaa tgctattgta attgtaaagg catgtgtaga
     7561 ctcagctatg caacttggat ttccagaagc gaaaatacca ttagcagaag ctactattct
     7621 tttggctaca tctccaaagt ctaattctgc atgtatagca ataatgaaag ctatggatga
     7681 actggataaa gagtttgtta aggatatacc aaatagtata aaggattctc actattctgg
     7741 ttctaaagaa ataggtaggg gttgtggata caaatatcct cataatttta aaaatcatta
     7801 tgtaaagcaa cagtatttac cagatagcat aaaagatagt atttattaca taccacaaga
     7861 aaataaaact gagaaaaata taaaaaaata tttagaatat ctaaatagtg atttttagac
     7921 tgaactttat gtatcggggg tagatattat gattataaaa cttaactcaa cttatcacag
     7981 tagagttatg aaatacttaa aaaaagaacc tgagtacaat ttgtttataa taggggatat
     8041 tgaaagatat ggatatggaa ataatttttt aaatatatgg gcagatgtgg gtgaacatgg
     8101 tgaaataaag gcaatattac ttaaatattt tgaatttatg atattttatt ctgatggaga
     8161 gtatgatgtt gaaggttttt ataatctact tcgtaatact aattacgaag aaatatctgg
     8221 gaaaatatgt gctgtagatg ctttagcaaa gagattagga cttaataatt taaaggtagt
     8281 tgatttttgc aaacttcaaa ctaaaaagtt tttgatagat aataattgta atgcaaaggt
     8341 taaaagaatt aggcttggaa atctaaaaaa gacagtgaaa ctttatgact taatagatga
     8401 atttcacagt acaactttag agaatttaaa aaatggacta aggacaggta gaggatattg
     8461 tatagagatt aacaaacagg tggtgtcaat ggcaaagagt acttcagaaa ataggactca
     8521 tgcaatgata attggagtag gaacacatcc taaatataga gctaaaggac ttgctacaaa
     8581 atgcctaatt aaactatgtt cagagttact gagagaaaac aaaattcctt gtttgtttta
     8641 tgataatgaa gaggctggaa agatatataa aaaactagga tttgaaaaca ttggaaaatg
     8701 gggaatttat tcaaaataaa aaattaaaat taatcttgac aaaattagta gggtataata
     8761 aaattatctt gaaagtaatt cctactaaaa cagtatgaat tagatttcta tatagaggtg
     8821 ataatatatg aagttgtcta ctaaaggtag atatggacta aaagcaatgt ttgaattagc
     8881 attaaatcaa gacaatggac cagtatcatt aaaatttatt gctaagaagc aaaaaatatc
     8941 agatcagtac ttagaacaaa tattttcatc tttaaaaaaa tcgggtctag taaaaagtgt
     9001 gagaggtgct caaggaggtt atttgctttc aaagaatgct gaagacataa cagtaggaga
     9061 tatacttgta gtattagaag gaccagttgc attatctgac tgtgtactag atgaagatgt
     9121 atgtgaaaac tcaaacatgt gtgtaacaaa aatagtttgg gaaaagatga aaaagggtat
     9181 agaagatgtt atagattcaa ttactcttaa agatatgata aatgactata acaaaaataa
     9241 attagaaaat gatataacaa atattaaaaa atagatttgg agatgaatgt aatggaaaaa
     9301 agaagattgt atatggatta ttctgcaaca acacctatta aaaaagaagt attggatgca
     9361 atgatgccat acctaacaga ttattttggt aatgcatcta gttttcacac attcggaaga
     9421 gaagcaaaag atgctttgga taaagcaaga gaacaagtag cagcccttat aaatgctgaa
     9481 cctagtgaaa tatattttac agcaggtggt tcagaaagtg ataactggac actagagggt
     9541 gtagcttatg ctaataaaaa caaaggtaat catataataa cttcaaaaat agagcaccat
     9601 gcaatacttc atacatgtga atacttagca aaacatcatg gatttgaaat aacttattta
     9661 gatgttgata gcgaaggaaa agtagactta aagcaactag aagattcaat aaaagatact
     9721 actatactta taagcataat gtttgcaaat aatgaaatag gaactataca gcctattaaa
     9781 gaaatatccg aaatagctaa aaaacataag atattattcc acactgatgc agtacaagct
     9841 acaggtaata ttccagtaga tgttaaagaa ttaggaatag atttgatgag tatgtcttca
     9901 cataaaatat atggaccaaa aggtgtagga gctttataca taagaaaagg tgtaagactt
     9961 cataactttg tccatggtgg agcacaagaa aaaagcaaaa gagctggtac agaaaatata
    10021 cctgcaatag ttggatatgg aaaagcagca gaattagcta aggaaaatat gcaaaatcat
    10081 gtagaaactt taactcgtct tagaaataag ttaatagatg gtgtgttaga aagaatacct
    10141 tacacaagag taaatggtag cttagataat agattgccag gaaatgcaaa ctttgcattc
    10201 caatttatag aaggtgaagg aatactttta ttattagata tgttaggaat tgctggttca
    10261 agtggttctg catgtacttc tggttcatta gacccttcac atgtacttct tgcaataggt
    10321 ttaccacatg aaatagcaca tggttcatta agacttacag ttggagattt tacaacagat
    10381 gatgatatag attatatact agagaactta ccaaaagtaa tagaaagact tagaagcatg
    10441 tcaccacttt atgatgatgc gaagaaacaa ggtttagtaa aatagacata aagataaagc
    10501 ataaaaaata gacataaatt gagtaggagg aaaagataat gcaatatagt gataaagtta
    10561 tggaacattt tatgaatcca agaaatatgg gagaaattga taatgcaagt ggtgtaggtg
    10621 aagttggaaa ccctacatgt ggagatataa tgaagatatt tttagacata gatggagatg
    10681 taataaagga tgttaagttt aaaacatttg gctgtggttc agctatagca agttcttcta
    10741 tggcaactga aatgataaaa ggaaaaacta taaaagatgc attagaactt acaaataaag
    10801 ctgttgcaga agctttggat ggtttaccac cagtaaagat gcattgttca gttttagctg
    10861 aacaagctgt aaaagcagcc cttatagatt atgctcaaaa gaacaatatc catataccag
    10921 aattggatgg atatgttata gatgatgctc atgaccatga tgttgaagaa gaagaataaa
    10981 aataagtgat tgtgttatga gaagtttaca atgattaaaa agaagtacta atgatataat
    11041 taggacataa aaaatagctg tttttaaaat ggctattttt tattgcctaa attaagtatt
    11101 ttaacttgat ttagaggtta attagttaac aaaataatcc ataagattca aataataaag
    11161 atgattattt ttttagaaaa tagaacaagc tatattttaa ataagtacaa tacttgctct
    11221 atatggtata ataataagta atgcaaaaga tgaggtgaaa tttgtttatg aataaaaaag
    11281 taatgatagg tatgagtgga ggagttgata gctctgtagc tgcatatctt ttaaaacaac
    11341 aaggatatga tgtcattggt gttactatga agttgtggca agatgatgat gttgttgaaa
    11401 ttgaaggtgg ttgttgctct ttatctgctg ttgaagatgc tagaagggta gcaaataaaa
    11461 taggtatacc attttatgtt ttaaatttta gagaagtgtt taaagaaaaa gtaatagatt
    11521 attttataga tgaatatttg gaaggaaaaa ctcctaatcc ctgtatagct tgtaataaac
    11581 atataaaatt tgatgatttt tataagaagg ctagacaaat aggttgtgat tatgtagcta
    11641 caggtcacta tgcaaagata gaaaaggatg aaagtacagg aagatatttg ctaaaaaaat
    11701 ctgttacaga taaaaaagac caaacttatg cactttataa tcttacacaa gagcaactag
    11761 agcatacttt attgccaata ggtgactatg aaaaagatag agtaagagaa attgctaagg
    11821 aaatgggtat ggcagtacac aataagcctg atagtcaaga aatttgtttt gttaaagata
    11881 atgactatgc aaattatgta aaaaaacatt ctaaaaagcg tattgaagaa ggtttttttg
    11941 tagatactaa aggcaatata cttggaaaac ataagggtat attatattat actataggcc
    12001 aaagaaaagg gcttggaatt acttttggaa aaccaatgtt tgtaatagat ataaatccaa
    12061 taaataatac catagttctt ggagacaatg aagatttatt taaaaaggaa cttattgcta
    12121 aagatgttaa ctttatatca atagatacgt tagaagaacc cttaagagtt caagccaaaa
    12181 taagatattc agcaaaacca tcaccagcta ctattcatag agtaggagaa gatactatta
    12241 aaatagtttt tgatgaagct caaagagcta ttactaaagg tcaatctgta gtaatgtatg
    12301 atggagatat tgtagtaggt ggaggtataa tcgagaaaag tttataaaaa catctaaaaa
    12361 attaaaaaaa taagcaataa agtatattaa gagtaaataa agtttataaa ataaatttat
    12421 tgtatatgaa aatatagtat tgattaattt tgtaaaattt agtaatataa ttagtatatt
    12481 aattaaaaaa caaatacagt gataaggaat agtaaaatat ttgatgtctt acagagagag
    12541 ggaaatgctg gaagccctta gaatatgata tttgaagcag ccttttagta gcaatcttga
    12601 acaatttatc ttttataaag aagatttaag tagagattga cggtagttac gttatagcta
    12661 aggttttccg aagatatcat actttgtatg ataattaggg tggtaccgcg attgtcctcg
    12721 cccctataaa tttatttata gggacgaggt tttttattta aacgaaaaaa taaaatttat
    12781 taggaggaac atgatatgga aaagatggga ttaaatgaaa taagaagtaa gtttttagaa
    12841 ttttttgagt caaaaggtca ttatgtagca aatagttatt ctttagtacc aaacaatgat
    12901 aagagtttgt tacttataaa ctcaggtatg gcacctttga aaaactactt ttcaggtgta
    12961 gaagtacctc caagtgtgag aatgtgtaca tctcaaaaat gtataagaac aggagatata
    13021 gaaaatgtag gtataactgc aagacatgct acattctttg aaatgatggg taatttctca
    13081 tttggagatt actttaaaag agagtcaatc aaatggggat gggaatttgt tacagaatgg
    13141 ctaaatatac cagaagataa gatatgggtg actgtatatg aagaagatga tgattcttat
    13201 gatatatggg caaaagaaat gaatttccca gaagaaagaa tggttagact tggaaaagat
    13261 gataatttct gggaaatagg tacaggtcct tgtggtcctt gttcagaaat atattttgat
    13321 agaggagaag aatatggatg tgacaatcca gactgtaaac caggctgtga ttgtgatagg
    13381 tatttagaat tttggaacca tgtatttact caatttgata gagatgaaga aggaaattat
    13441 agtctattag aaaacaagaa tatagataca ggtatgggtc ttgaaagaat gggatgtatt
    13501 atgcaaggcg tagacactat ctttgaagtt gatacaatta aatctatact agaagcagtt
    13561 gagaagttaa caggagtaaa atatggagag aatccaaaaa atgatatatc aataagaata
    13621 ataactgacc acataagagc tgtaacattc ttagttagtg atggagtgct tccatcaaat
    13681 gaaggaagag gatatgttct tagaagactc cttagacgtg ctgctcgtca tggtaagtta
    13741 ttaggagtaa aagaattatt tttacagaaa ctaatagatg aggttataaa agtaaatgat
    13801 aaagcatatc cagttttagt tgaaaaagaa agttatatta aaaaagtagt tggaatagaa
    13861 gaagaaaaat tcaatgaaac tatagaccaa ggaacagaaa tattgaactc ttatatagaa
    13921 gtattaaaaa atgaaggaaa gactgtccta agtggtcaag aagcttttaa actatatgat
    13981 acttatggct tccctataga cttaactaaa gaaatattag aagaagaaca tctatctgta
    14041 gatgaagaag cttttaatga agaaatggaa aagcaaaaag aaagagctag aaatgctaga
    14101 ggaaatatgg atggagaaag ttggaaggaa gaccctctat caaaattaga atctacagtt
    14161 gacagtacat ttaatgggta tagtgaaatt tatggtgaag gaactattga agctatagtt
    14221 aaagatgatg agctagttca aagtgcagaa gaaggcgata aggtatctat agtactagat
    14281 aacactactt tctatcctga aggtggagga caagttggag attgtggatt gataactaat
    14341 gaaaacttag ttttagaagt attaaatact aaaaaaggtg ctaataatag tataaaacat
    14401 ataggtataa taaaatcagg aagaataagt aatggggaca aagtaaagac actcgtagat
    14461 agagaaacta gaatgtcagc tgcaagaaat cacagtgcta cacatttact tcataaggca
    14521 ttaagagaag tattaggaga acatgttaat caagctggtt ctttagtaac tccagaaaga
    14581 cttagatttg atattactca ctttgaagca atatcaaatg aagaattaaa agtaatagaa
    14641 gaaaaagtaa ataatgttat tttatcatct ttagacataa aatgtgatat tatgaatata
    14701 aaggaagcta aagaaaaagg agctacagca ctatttggtg aaaaatatgg tgatgaggta
    14761 agagtagttt ctatgggaga ttattctaca gaactttgtg gaggaactca cttgacaaat
    14821 acttctcaag taggaatgtt caaaatatta tctgaaggtg gagtagcagc aggtgtaaga
    14881 agaatagaag caataacagg aaaagctgtt tatgaatacc taaaagaaag agatggaatt
    14941 atctctgaag tttgtgtaaa cttaaaatct aaagaagata atttaattca aagaataagt
    15001 tctttattag aagaaaataa aaacttatct aaagaattgc atgatatgaa agctaagatg
    15061 agtttacaat cagttgattc tatttttgat tctaaagttg aagtaaatgg agttaatttg
    15121 ataactaata aatttgaagg tatggatatg gatacactaa gagaaacagc agataatcta
    15181 agagataagc ttggttctgg tgtagtagta cttgcaaatg tagtagatga taaagttaac
    15241 tttgtagtta ctgccactaa agatgtatta gataagggaa tacattcagg taatattgtt
    15301 agagaagttg caaaaatagc tggaggaaaa ggtggaggaa gacctaatat ggcacaagca
    15361 ggagctagtg atgtatctaa ggttgaccaa gctttaagct atgcaagtga ggttattaag
    15421 acacaagtta aataacattc actcaaggag gtttttagtt tggaaaaaga tttagactac
    15481 actatgaaat ttgaaggaat accagaagat aggatgagtg taggagatac tattgatttt
    15541 gtatataaag cattagttga aaaaggatat aaccctataa atcaaattat agggtatctc
    15601 ctttctggtg attctagcta tataactagt cataaaaatg caagagctat aattaaaaaa
    15661 tttgaaagag atgagatact tgaagaagta atcacgcact atctgaatag aaagtaggtc
    15721 aaataaatga gaaaaaagat tatgtcgttg tttgcaatag tcattatgat ttttgtatca
    15781 actgctagct atggttttgc tgaagaaaaa ggcaaggtca tatttattga tatgaatagg
    15841 accaatcttg gaagtatgat gaatattcca atcttgaaaa atgagataga aaagagaggc
    15901 tatgtagctc tcatgaatat aagaggggat caaggtactg atgatagaag gtcttgtgca
    15961 agtatgggtg caggaacaag agctagtgta actactgaag actatatcaa ttttcaaaaa
    16021 gtagaccaag atactgctaa agcttttgaa gtcgcaacag gtcaaaaagc taaagaaata
    16081 aatgatttag gcattaatag atctataaat gaaaatcttg agtatggaga atatggagca
    16141 aatttagggt cattaggaaa aactttatca gaaaatggtt taaagacatc tgtaattgga
    16201 aatgctgata taattgaaaa taatcaatta gttaaaaaca gaaacttatg tttagtagct
    16261 atggaccaat atggaaggat tgattcaggt aatattgata atattaataa gaaagattct
    16321 aaaatgccat ttggaatatc tacagattat gataaattac tagatgaaac taaaaaatcc
    16381 tataaaaata gtgatgctat atttgtagaa cttggagata catatagatt agatatgtat
    16441 agattaaatc taaatgataa tacttatgat acaatgaaga aaaatattgt aaaaaatata
    16501 aataaatatc ttgaacaagt attttctatg gttggagaaa atgacactgt ttatatagca
    16561 agtgcatttc caagtgacct agattataag aataagagaa gactttctcc aataataaaa
    16621 tttagtggag aagggaaagg tctactggaa tctgccacaa caagaaaggc tggaattgtt
    16681 gccaatttag atgttggagt agatatacta ggtacatttg gtcttgaaaa taaagagatg
    16741 gtaggtagaa gttatacttt agtaaataaa gaggacaata ttagctatct aaaatcactt
    16801 tatgaaagga tagtgtctat atcttcaata aggtcaacta ttataaatgg ctttgtaggc
    16861 gtaatatctg cttcttgggt aattgccatg tttgcgattt tctttagaaa ttatatgcca
    16921 cataaagagc gtgtattcaa agtacttaag gagtttataa aattaggcat aataatgcca
    16981 ctatcattta tgttagctcc tttgatgaat tttaaaactc caatttcaat atctttggga
    17041 atatttgcaa ctacgctaat attatacttg ttaggtagaa aattatttaa ggacaatgat
    17101 ataaagcaca tgggattttt tgcaacactt actattttag tcatagttat agattctgta
    17161 tttggaacat acttaatgca aaataacata atgagttatg atgctgtcat aggagctagg
    17221 tactatggtg taggaaatga atatgaaggt gtaactatag caagtgcagt atttggctta
    17281 gcagtattat tacattataa gaaggtatct aaattacttg ttgtaatttt ttcattaata
    17341 atacttataa ctagtgcata tccttcaatg ggtgcaaatg ttggtggagc tatatcagaa
    17401 tgtgtagcat atttgttgtt tataatgctt atatatgaca ttaaaataga ttttaagaaa
    17461 gctgtattat tagctgtttc agctgtagtt gtcgtttttg catttgcagc tttagatata
    17521 ctttcaggta gtgaatctca tttaggtatg tttgtacaga gaattttcct aaatggacca
    17581 ggagagatat tacaaacatt tggaagaaaa attcaaatga atatgcaact agcacagaca
    17641 agtgtatggg taaatatttt acttgtagga attggagtaa ttgcagtgct tatatttaaa
    17701 ccatctagac actttagaaa gataatgaat aattttccaa tcttgtttaa agggtttatt
    17761 gcatctatgg ttggttgtat aattactctt ttagttaatg attctggaat agttgctgca
    17821 tctacagcat ctatatatat acttatacca ctaataataa taagtataaa tatgataata
    17881 tttgaaaaaa ataactaaaa aaatttttac tgttcatata ggatttaaat aaaaaatgca
    17941 atatgattat taatgtaaac tggatgctta cataaaaaat catattgcaa tttttatgat
    18001 tgataatgta aaattctatg aagcagtaag cctgtgacct tagttatgag aggttcagaa
    18061 tatgtattta atctatagaa tataaataga taaaggagga actatgttag atggaagaat
    18121 aatgggtctt gatgttggtg ataaaactat tggagttgct gtgagtgatt tgatgggatt
    18181 aactgcacaa ggtgtaaaaa caataaaaag agttggcaag aaaaaagata ttgaagagct
    18241 aaaagcaata ataaaagaaa aacaagtaaa taaaattgta tcaggattac caaaaaatat
    18301 gaatggaact ttaggtccac aaggagaaaa agtaatcaag ttttgtgaat tggtaaaagc
    18361 agaaacaggt attgatgtag agttttggga tgagaggctt tctacagtag cggcagagag
    18421 gtctttatta gaagcagatg ttagtagaca aaaaagaaaa aaggttatag atatgttggc
    18481 agcagtaata attttgcaag gatatttaga ttttaaaata aattcataaa agaactataa
    18541 aattgaatac ttatgatata taatataaat aataaaaata aaaataaatt tgaggtgaat
    18601 gacattatgg aagaaaatat aataaattta atagacgaaa atggcgttga aagccaattt
    18661 gaaataatat taacattaga agcagaagga aaagagtatg caatattaat gcctttagat
    18721 gatgaagaag ctgaagaagc tttaatcttt agaattgatg aggatgaaga aggtgaaatc
    18781 ttaatacctt tggaaagtga tgaagagtat gaaactgttg tagctgtgta tactgctata
    18841 atggaagaag aaggtttaaa ttacgacgaa gatgaatcaa atggacttaa ctagataagt
    18901 aattaagttt gtatataaaa atcagatatt tattatattt gctttaatta aaccttatta
    18961 attgtaaaag catttatagt atatatctgt ttttttgttt tttattaaaa ataccaaaaa
    19021 atttatgaat tttggtgaat aatatatctt ttatgggtat atatttatat tatgagaata
    19081 aattaaattt atttaaatgt cttgacaaaa aatgaattat caattacaat attatttaat
    19141 aaagattatc aattgaaaat agtaagggtg atgttatggc aaatacaatg gatttattaa
    19201 aagacaagtt aaaagaaact ggttttaaga ttacgccaca aagaagagca atagttgaaa
    19261 tattgcttaa acatgaccat tcacatctaa gtagtgagga aatctatgat ttagttaggg
    19321 ttgactgtcc tgaaataggc ttagctactg tatatagaac aatgcagcta ttggatgaaa
    19381 taggattaat atcaaaacta aacttagacg atggatgtat aagatatgag attagtctac
    19441 ataaagaaga ctgtcataat catcatcatc ttatttgtaa aaattgtgga aagataatgg
    19501 aagctaaaga agacctttta gacaatatag aaaaagagat acaatcattg tacaaattta
    19561 aaatacttga tcatgatgtt aaattttatg gactatgtga cgagtgtaat ggagtaagcg
    19621 attcagaaga ataaacttct attgcttaat gtcgatggaa gaatagtata gaacttttat
    19681 attctatgct attcttccat tttttattat aaaccatcta taaaatgcaa atatacagtt
    19741 ttcaaagtga ttatagtttt aatattgaaa atataattaa tcctataagt acaaaaatga
    19801 atgatttttc tatgaaatta ctaaatggtc ctccaacagt taaaaaccct aaagaaattt
    19861 tcttttcgct taggggataa aaaattggta ttccttgctt tgtaaataaa tctcctaaaa
    19921 acatatggct tgcataacca atcgtaccat aaaaagataa attattaata ttagtgatta
    19981 attctatctg ttttagtaaa aaatatacta ttatagttgc aaatatgcta tgagaaaaac
    20041 ttctgtgctt taaccagggg aaaattgaca acattagaca aaaaattgta aaatatattt
    20101 caacttctat gatgtataag catatagcta atagtataaa tattaatgat agaaaaactt
    20161 ttcttagaaa tgtatgggtt agttttgctt caataattat aattgcaaca aatgtaatta
    20221 ttgcgcttaa ataaaaatta tcatttatat ttattgaaat aaagaaaata ataatattaa
    20281 cagcatatat aaaaattcta tatatgtatt tggatacatt gttttttaga atgaaatttg
    20341 agaccataga attagattta tccaaatcgg gtagtacagc aaaagtagct gaaactaaaa
    20401 tatcaactaa agatatagga attttaaata ttaaagatgt ctggatagct gttaagattc
    20461 ctattgcaca atgagtttta ccacgcataa atcctcctaa attatccgat attaaatcat
    20521 ataatattta gaggatatca aaataaaata ggtttgtcaa aataatacgt taaatgtatt
    20581 ttaaatatag cttaaaatat gatacgaatt atggtacaat aagaataact ggtagcgagt
    20641 gcgtgatttg atattattta tgattgtaga acgtgttaaa aaattaaata gacagaagga
    20701 gatgatgcaa ttgttcaaaa agaacaccaa taagataaag gtaatggcct tgggtggact
    20761 taacgaagtt ggaaaaaata tgactgttgt tgagtataaa gatgaaataa ttgttataga
    20821 tgctggactt agttttccag aggatgaaat gttaggagta gatatagtta taccagatat
    20881 aacttattta gtaaaaaata gggataaaat aaagggtata tttattacac atggacatga
    20941 agaccatata ggagctcttc catatatatt aaagaaaata aatgtacctg tatatggggc
    21001 aagacttagt ataggtctta tacaagtaaa acttaaagaa cataagatga ataatgttaa
    21061 gttaaatgtt ataggtccta gacaagttat aaagcttgat aatatggaag tagagttttt
    21121 gaaaaataac catagtatac cagatgctta ctctattgca atacacacag accaaggtat
    21181 aatataccac acaggagatt ttaagattga cttgactcct attgatggag atgttatgga
    21241 tatgcataga atctgtgagt tgagcaaaaa aggagttctt ttaatgctgg ctgatagtac
    21301 taatgcagaa aaaccaggat ttactatgtc tgaaaagact gttggggtag gtcttgacga
    21361 attatttgca aaaggaaatg gcagaagaat tatagttgct acctttgcat ccaacataca
    21421 cagattacaa caaataataa atactgctga gaagtttaat agaaaagttg ctatatcagg
    21481 tcgctcaatg gtcaatgtag ttggagttgc aaaagaatta ggatatctag atatttctga
    21541 tgacatgctt atagatttaa atgatatatg taagtatgaa gatagtgaat tagtgataat
    21601 aacaactggt tcacaaggtg aacctatgtc agctcttgct agaatggctt tttcagagca
    21661 caaaaaagta gaaataaaga gtggcgattt agttataata tctgctcatc caataccagg
    21721 aaatgaaaaa ttaatatcaa gagtaatcaa tttcttattt gaaaaaggag cagaagtagt
    21781 atacagtgat atagctgata tacacgtttc tggacatgcc tgtcaagaag aattaaaact
    21841 tatacatgca ttagttagac caaagttttt tatgccagct catggtgaat atagaatgtt
    21901 aaaaagacat gctgaaatag ctgaacagct tggtatggat aaagaaaata tttttgtcat
    21961 gcaaacagga gatgttttag aattagataa aaactctgct aaggtagcaa atcgtataca
    22021 aacaggaaac atattagttg atggtctagg agtaggcgat gtaggtaata tagttttaag
    22081 agatagaaag catttatcag aagatggtct tatgatagtt gtagttacta tttctaagga
    22141 tgagggtaaa gttttagcag gtccagatat aatttccaga ggattcgtat atgtaagaga
    22201 atcagaagat ttaatggatg gagcaaagga tataattaag aatgttctaa atgaatgtga
    22261 agagaaaaat attaaagaat gggcttattt aaagaataat attaaagaaa acctaaaaga
    22321 atatttatat caaaaaacta aacgaaatcc aatgatactt cctatcataa tggaagtata
    22381 atataaaaaa actataaatt tgaaaattag acctcttgta atttttaaag taatatcaaa
    22441 aatccaattg atattatctt aagaattaca agaggtcttt ttaatacttg atttaaaaaa
    22501 atatatatat tacataaaat accaattata acaatctttt attagtaaaa actacttata
    22561 tataaatttt actgatagga ggattcttat ggatgatgtt tcaagacaaa atgctataaa
    22621 agctttaaaa caaacaaaaa tggaaatagc aggagagtat ggaatgaatt atgaagatgc
    22681 ttttgaaata atagaaaatg cgtctaataa aggtgtttta gaaggttact ttaaaaagct
    22741 tgaaaagaaa aaaaatttag gtcaaggtat atctaggcat ttagaataaa aaaagttaaa
    22801 tgtgagaaaa taagtttaaa taaactttaa gaggtgacat cgatgaaaaa attagctagt
    22861 actgctttgg ctatattaat tgcgcttact cccttaagct tttcttttgc aaacaataag
    22921 gaaaatgctg atgccaacca actaaatata tcttcaaaat cagcaatttt gatggatgtt
    22981 ggtagtggac aaattttata tgaaaaggat gctcataaaa agcttcctcc tgctagtgta
    23041 actaaggtaa tgactatgct acttatagta gaagctttag attcaggcaa gattaaactt
    23101 gatgatgaag ttcaagtaag tgaaactgca tctagtatgg gtggtagtca aatattccta
    23161 gagccaggag aaactcaaaa ggttgataca ttgttaaaag gtatagctgt tgcttctgca
    23221 aatgatgctt gtgttgctat ggctgaacac ttagctggaa gtgttgaagg gtttgttgat
    23281 agaatgaatg ctaaagcaaa agagttaaat atgaatgata ctcattttgc aaatacaaat
    23341 ggacttccag ttgcaaacca ctatacatct gctcatgata tagctttaat gtctagagaa
    23401 ctattaaaac acgaaatgat aagtaaatac ttaactacat ggatggataa ggttgtagta
    23461 gggaagaaac aagttacagt aggtcttgca aatactaaca aacttataaa gcattatcaa
    23521 ggagcaactg gtgttaaaac tggctttact caagaagcga aatactgttt atcagcatct
    23581 gcaaagagag gtaatactca tctagttgct gcaactttag gagcagagac ttctccagaa
    23641 agatttaatg atgcctctag ccttttaacc tatggatttg ctaattatga aagtgttaag
    23701 ttatgtagta aaggtgataa tatagcaaca cttacattgg ataaggctga tgaaaacaaa
    23761 gttaaattgg ttgctaaaga agatttaaat gctcttatta aaaaaggaag ttctaaagaa
    23821 tttgagaaga aaattgaaat agtaaaaaat cctaagatgc caattaaaaa aggaactgtt
    23881 ttaggaaaga taaagatatg taaagataaa aaagtaatag gcgaagtgga attaataaat
    23941 actaaagata taaataaggc aagctatcta caaatgcttc aaagaataat tgataatatg
    24001 atataaataa ttatcagatg aaatataatt ataatacaca aagatatgat tataatacac
    24061 ataaaaaata cccttatgta attatttgcc ataggggtat ttttatttac tatatagaag
    24121 tattttataa tcttatgatt ttta
//
LOCUS       DBGJFU010000053        24097 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.053, whole genome shotgun sequence.
ACCESSION   DBGJFU010000053 DBGJFU010000000
VERSION     DBGJFU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.053"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..946)
                     /locus_tag="ACOIAS_002002"
     CDS             complement(<1..946)
                     /locus_tag="ACOIAS_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2701714.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKY"
     gene            complement(933..1700)
                     /locus_tag="ACOIAS_002003"
     CDS             complement(933..1700)
                     /locus_tag="ACOIAS_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701715.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            1988..2662
                     /locus_tag="ACOIAS_002004"
     CDS             1988..2662
                     /locus_tag="ACOIAS_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2701716.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            2686..3690
                     /locus_tag="ACOIAS_002005"
     CDS             2686..3690
                     /locus_tag="ACOIAS_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGZ2701717.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            3802..5031
                     /locus_tag="ACOIAS_002006"
     CDS             3802..5031
                     /locus_tag="ACOIAS_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGZ2701718.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            5039..5713
                     /locus_tag="ACOIAS_002007"
     CDS             5039..5713
                     /locus_tag="ACOIAS_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701719.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            5716..6948
                     /locus_tag="ACOIAS_002008"
     CDS             5716..6948
                     /locus_tag="ACOIAS_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701720.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(7234..8499)
                     /locus_tag="ACOIAS_002009"
     CDS             complement(7234..8499)
                     /locus_tag="ACOIAS_002009"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGZ2701721.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(8552..9700)
                     /locus_tag="ACOIAS_002010"
     CDS             complement(8552..9700)
                     /locus_tag="ACOIAS_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HGZ2701722.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNKVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(9800..10345)
                     /locus_tag="ACOIAS_002011"
     CDS             complement(9800..10345)
                     /locus_tag="ACOIAS_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HGZ2701723.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(10568..11512)
                     /locus_tag="ACOIAS_002012"
     CDS             complement(10568..11512)
                     /locus_tag="ACOIAS_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2701724.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            11898..12779
                     /locus_tag="ACOIAS_002013"
     CDS             11898..12779
                     /locus_tag="ACOIAS_002013"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2701725.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(12840..14048)
                     /gene="tyrS"
                     /locus_tag="ACOIAS_002014"
     CDS             complement(12840..14048)
                     /gene="tyrS"
                     /locus_tag="ACOIAS_002014"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGZ2701726.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(14121..14373)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14525..15493)
                     /locus_tag="ACOIAS_002015"
     CDS             complement(14525..15493)
                     /locus_tag="ACOIAS_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HGZ2701727.1"
                     /translation="MNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDFIVPVFFC
                     IICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINKPTMVMDD
                     LVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLMIALFPFG
                     MVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICVSYLAIFE
                     VLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYARILFLII
                     ILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            15666..16106
                     /locus_tag="ACOIAS_002016"
     CDS             15666..16106
                     /locus_tag="ACOIAS_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2701728.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            16433..16660
                     /locus_tag="ACOIAS_002017"
     CDS             16433..16660
                     /locus_tag="ACOIAS_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2701729.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            16686..18806
                     /gene="feoB"
                     /locus_tag="ACOIAS_002018"
     CDS             16686..18806
                     /gene="feoB"
                     /locus_tag="ACOIAS_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HGZ2701730.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(18939..19757)
                     /locus_tag="ACOIAS_002019"
     CDS             complement(18939..19757)
                     /locus_tag="ACOIAS_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2701731.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            19976..20248
                     /locus_tag="ACOIAS_002020"
     CDS             19976..20248
                     /locus_tag="ACOIAS_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701732.1"
                     /translation="MKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKAVKLE
                     IFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   20405..21228
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=20472..20500
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(21887..23980)
                     /gene="pdcA"
                     /locus_tag="ACOIAS_002021"
     CDS             complement(21887..23980)
                     /gene="pdcA"
                     /locus_tag="ACOIAS_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdcA"
                     /protein_id="HGZ2701733.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         8575 a         3626 c         2951 g         8945 t
ORIGIN      
        1 tgtatttata attttcactt atgttcctat ctatatcgta ataaagtgat atggtaaacc
       61 ctagcatagt tacacttgta gctattaata ttgctattac agccaataca ctactattgc
      121 tctttaattt aaatccaagt tcagaaaatg caattaaatt ctgtccctta taataaaaac
      181 tttttctctt cttaacagca tcaataatca tatacatagt tgatgaaaaa aataaaaaag
      241 ttccaggtat tattgtaact aatataaaaa ctgaaagcat aatattttca gcaataaaat
      301 aactaattga cattaaataa gaaatagcta ttaataaaac accaagtaca cattttaact
      361 ttataaattt tttaaactta tacttttctg gatatttatt aaataactct ataattctct
      421 tatttctgat aactatagta tttcttattc caataactat gaatattatt aaaaataccg
      481 ctagtgtctg aactaaggcc tttatactta agttcatatt tactactatc atttcattca
      541 ttaattttac aagcatcata gtcataagct ttgaaaataa cagtcctaaa aatataccta
      601 ttagtattgc aactataccc atcaaaaaag tttcatataa aaatattttt cctatctgat
      661 ttttcctcat tccaagtaaa ctataagttc ctacttctcg cctacgtcta ttgaaaaaaa
      721 atgagtttga ataatatata aataaaaata caaataccac tattacaatt gaacttgtat
      781 taatactcat cctagttttc tctccaagtg aactcaacgc ttcattatac tctatagatt
      841 taaaactaaa aaatacaaat acactaaata caattgaaat aaagtacatt aaataattaa
      901 aaaaactatt ctttacattt tgcattgcaa ttctaaataa agtcattttt attaactcct
      961 ccaattaagg aaactgtatt tactatttta ttatagaact ctctgttact ttctcccttt
     1021 acaatttctg tgaatattat tccatccttt ataaagagta ttctattgca aaaactcgca
     1081 cttatagcat catgagttac catcaatatt gttttattgc tgtctttgtt taattttaat
     1141 agacaattca acaaatccct agcagattta gaatctaatg ctcctgtagg ctcatctgca
     1201 agtactaatg atggttttgt tgctatagct ctacatacag ctcctctttg cttttgtcca
     1261 ccagaaactt cgccagggta tttatctaat atagattcta tatttaattc ttttgaaatc
     1321 tcttttattc tattttctat tactgatact ctttcatttt ttaaagaaag aggcaacatt
     1381 atattttctt ttattgtaag tgtatccaaa agattagaat cttgaaatac aaagcctaga
     1441 ttatcacgtc taaaatctgc caattcattt tgcttcattt taatagtatt ctttccattt
     1501 atataaatat ctcctgatga aggtaaatct attgtagaaa taatatttaa aagtgttgat
     1561 ttaccagcac cggaagaccc cataatacca acaaattctc ctttataaac cttgaaagtt
     1621 attcctctta gagcttcatg ttttgaacct tttcttccat actccttttt tatattttca
     1681 acagataata tttcttccat agttaataat cacctttcat cacaattagt tatttatcaa
     1741 ttctcacaaa tatattatat atattaaaat ggcttttaaa catttaatta tattaattta
     1801 aggttacatt ttcgtaagta ttaattatat ttcttcaaat ttattgcaat tatgtttaaa
     1861 atataattta tcaagtaaaa tatagttata ttaatataat tattttaggt aataaggtag
     1921 aaatatttta atttatcaag tagaatatag ttatgttaat ataactattt tgaattagga
     1981 ggtaagaatg tttaaaataa tggtagttga agatgacgtc tcattgaaaa atatcatagc
     2041 aaagtgtctt actaaatggg gacatgatgt tcatcaaata gaaaatcttg aaaatataat
     2101 agaagagttc aaaaactata acccagaatt agttttattg gacataaacc ttccatttta
     2161 tgatggtttt cattggtgta atgaaataag aaaaatttca aaagttccta taatttttat
     2221 ttcttcaaga aattctaata tggatgtaat aatgggtgta aatttaggtg cagatgatta
     2281 tatacaaaaa cctttttctg tagatgtctt agtagctaaa gttaatgccc ttttaagaag
     2341 aacttataat tttgttgata ataactcaaa tcagattatt cacaatggtg ttactttaga
     2401 tttatcaact gcaacaataa actatgaaga taatactatt gagctaacaa aaaatgaaat
     2461 caaaattctc catgagctta tgaaatataa ggggcagata gtaagtagga ataaactcat
     2521 gaaaaaatta tgggataatg attggtttgt agatgataat acattaactg tcaatgtaaa
     2581 tagaatacgc tcaaaattaa atgaaattgg tttagaagat tttatagaaa caaaaagagg
     2641 attgggttat ataatcagtt aaaacaattc taagtaggtg aatttatgag ctttaaagat
     2701 tttctcaagg ataaactagg ttttttagta tacaatttta ctttcttaat ttttacagta
     2761 tcagttatta tattttctcc tgtagatgtt tttctgactg atactatttt atatatatta
     2821 gttgtaaatg ttgtgttttt atttttatat ctatttatta gctacatcaa gaagaacaaa
     2881 tttttagata agataaaaaa tgatacattc aaaattaata tgaatgatat agaattagct
     2941 agatgtaaaa atgaagaaaa aatatacttg aacattatta aaaatcatca atatcaatgt
     3001 aataacataa taaataataa tatcgagaaa tttgaagaaa acaaagagat tttaaatatg
     3061 tgggtacatg atgtaaagat acctatagct ataataaaac tcattcttga acaaaatgaa
     3121 aatttaattg atgaaaaaat ttcagatgac atagatagtg aaatatttag aattgaaaac
     3181 tccgttgaaa aaattttata tttatcaaga ctcgaagatt ttcataagga ttttttaatt
     3241 caagaggtaa atattgaaaa aatcattaga aatattataa aaaaatactc aaaatatttt
     3301 atttcaaaaa aaattatttt agacttaaat aatttgaatt ttaaggtttt atctgatgaa
     3361 aaatggctat acttcatatt cgagcaatta ttgagtaatt ccctaaaata ctcctcatta
     3421 ggagataata tatctatcta ttgcaaaaga accaaaaatt atttacaatt aagagtggaa
     3481 gatactggtt gtggtataaa aaaggaagat ttaaatcgaa tattcaataa gggatttaca
     3541 ggtaataatg gaagaaacaa ttctaaatct acaggtcttg ggctttattt agtaaaagaa
     3601 ctttgtgata agcttggtca taaaattgat gtagattcag agtacaacca atatacaaaa
     3661 tttactatta cattcaaatg tgatttatag tatcttatta ttgatagttt attttcatta
     3721 acttatattc aacttcttat agtaattcta tatgtacatc ccagtatata attaagtagt
     3781 ttttataaaa aggaggaaga catgtctttt ctaaaaaaat ttaaaaataa aagatttaga
     3841 aataacagtt ttaaaagtaa caactttaga aatagcagtt ttggaagtag tggcttcaag
     3901 tctaaaaata aaggttttaa aaacaatccc tttaaaaata agagattcaa aatatatgca
     3961 ggaatagcag taatcatact tttaattcta ggagttcttg cttatgttga ttcgaaaaac
     4021 actgaattac aagctaatga aaattttatc gaaacatata ctattcctga aaatgaaaaa
     4081 atatttataa atgggatggt tgttcctaag caaactaagg acttcaatat atctggagat
     4141 tatgagttaa gtgatgttaa tgttacaaat ggtcaaaaag taaatcaagg agaccttctt
     4201 tttactgcta aaaatccaac tataatagca gaaatagata gtttaaaatc acaacttagt
     4261 caatacaaaa aacaaaaaat atcactttct gatattgctg aaaataaaga tgctattgct
     4321 tctataaatg cacagataac tgctttaaac tctcagatag cttctctgga taaaaaagct
     4381 tatgacagag ttactgctcc ttttgatggc acagtatact tgaatgacca aactggaaat
     4441 ccagaccaac cagtttcatt tatgactatt caaggtctag agttttatat gaaaggacaa
     4501 gcaagtgaac aagatttacc taaaatgaag attgaccaaa tggtcaatat actagttttt
     4561 tcaactgacc aaaagcttac tggtagaata agttttatat ctgataaacc atctactcca
     4621 aatactgaaa tgggagctca gcagaataca ctatcttatt atgatataaa catagcattt
     4681 gataatcaag aaggattagt aaatggattc catcttcaag catcacttga agtagctaat
     4741 tcaagcttta aaataccagc atcttgtgta cttaaagaca acaaacactc atatgtattt
     4801 aaggatttag atggtatatt gaaaaagcaa attgtagatg tagcaagtca aaatgatgat
     4861 tttgctgtag ttagaggcgg tcttgaacaa ggcgatataa taataaaaca ccctactaaa
     4921 gaaatgaaag aaggagaccc agttcaaggc gatggcgtaa ctgctggttc taataatgga
     4981 gatactactc caaacaaaaa agtagaagaa gttactatgg atgtgaatta atttacatat
     5041 gttaattaaa ttagaaaata ttcaaaaata ttacaaagta gggaaagatg aattacatgt
     5101 tttaaaatcc ttaaatttag aaattgaatc tggagaattt gtcatgataa tgggtaagtc
     5161 tggtagtggt aaaactactt tacttaatat cttaggtttt ctagatgtat ttgatgaagg
     5221 aagatatata tttgatggaa cagatgtaac taatttgagt gaaaatgaac gttctgtttt
     5281 tagaaatatt aatattggat ttgttttcca acaatttaat ttaatagaaa ctctaaatgt
     5341 atatcaaaat gtagagttgc ctctaattta caataaagca ctaaaaaaat ctaatagaga
     5401 agaaattgtt aaagataaat taagttctgt tgggcttttg gacaagctta agcagaaacc
     5461 acttcaatta tctggtggtc aacaacagcg tgtagccatt gcacgttgcc tagcaaatga
     5521 ccctcagata atctttgctg acgaaccgac tggggcatta gatagtgaaa ctagcagaga
     5581 aattatggag cttttaacaa ggctcaacaa acaaggtaag actataataa tggttacaca
     5641 tgatcaagat ttaactaagt atgcaactaa ggttatacga cttaaagatg gtgtgtttac
     5701 ttcggaggtg taatcatgag tttattaaaa aattctttgg ccaatttaaa aggtcacaag
     5761 cttcgtgtat ttgtagcttt actctggatt ataataggaa taacttcagt aatacttgtt
     5821 agttctatag gtaatggatt tcaaaaagaa attaagaaat ctgtaaacaa tgtaaaccct
     5881 aataaaacta ctatttcgtt tgaatcagcc gataacacag gtcttactga tgatatgagt
     5941 atttttttaa aaccctttaa tgcaaaagat ttagaagaat tatcatttgt tgaaggagta
     6001 gaaagaatag ctccttcaag agatgggttt aaccttgatt cagtttactc ttcacaagca
     6061 tcttttgata aaaaaactac gtatgttgat gttggaccag taaaaaaaga ttctaaaata
     6121 aatttaattt gtggtagaga tttttcttta gatgatgaaa aaagaaaagt aattttactt
     6181 acattacaaa gtacaagtga aatttttgaa aatccagaag atgccttagg tcatggtatc
     6241 aacataaatg gaactatatt tgaaataata ggggtattag atgattctca gcaaaaccaa
     6301 gctggaggat tctttggagg ttaccaagat atgcaattta ctacttccct agtacctaaa
     6361 aaggcttttg acactttaat gagtcaaaat tcttattcaa atgaaattta ccagttagac
     6421 ttagtttctt ctaaaggcta taatgtaaat gaagttgcaa acaatgttat agctaaatta
     6481 tatgagatgc accctggtat caatggttct tatactacac cagacccaac tgaacagaca
     6541 gcttatcttg aaagcattaa ttccaatgta aacaaatatg tatctataat aactgtagtt
     6601 gcaatgtttg ttggtggtat tggtgtaatg aatataatgt atgtatctgt tatggaaaga
     6661 caaagagaaa ttggtataag acgtgctatt ggagcaaaac ctaggtctat attatttcaa
     6721 tttttagttg aagctgtttt tataacagta tgtggtggaa ttttaggaac tatagttgga
     6781 tttgctgcta ctaactatgt ttctaaatat ataggttttg aagctatacc tagcttaaat
     6841 agcttatttt atgcaattgt agctactata ttgactggag ttgtatttgg tctgattcca
     6901 gcctttaaag cttctaaact agaccctata aaggcaattt ataagtagaa attgtataaa
     6961 gattttcgat ttttagatta gtttatataa aacatactta aatatataac gcactattat
     7021 atatgataga ttaggaactt aatctttcca aatataatag tgcgttcttt cataaaaagg
     7081 attctcttcc ttactatatc taaattacca agatgttatg ttctaattgt tcaaactaat
     7141 acttccaatt ttgattaatc aatctcattc tcctaatata caatctttta ctttcaatca
     7201 ttgtacactc cgccttaaaa aaataatctt aaattaggcg ttatatacaa tctccccatt
     7261 acatattgta taattaacct ttccatataa agatttacct ttaaatggtg aattatcagc
     7321 tttagaaaca aagctttcaa ctgtccattc ttcttctgga ttgaatataa caatgtctgc
     7381 tacagcccct tcttctataa atccaatatt taaattgtac aattttgcag gatttataga
     7441 cattttttct attaactgca tcatactaag atgattttta tgcaccaaat ttgttattcc
     7501 aagagctaaa gaagtttcaa gcccaataat tccacttgga gcttttataa actctctatc
     7561 tttttcttct ttactatgag gtgcatggtc tgttgctatt atttcaatag taccatcatt
     7621 taatccctct ataatcttat ctctgtccca tttacttctt agaggtggat tcatttttgc
     7681 atttgttcca aactccaata catcttcctc agtcaatgta aagtgatgtg gtgaagcttc
     7741 tgcaactaca tttgcaccta agtattttgc aaatctaacc atatcaacag atacacctga
     7801 acttatatgt tgtatatcaa ctttagcacc agtctttagt gccaacatac aatctctggc
     7861 taccataaca tcttctgcta cacttgatgc accttttaat ccaagttctg aagctacctt
     7921 tcctgcattt acacctggat ttccaactaa agaagggtct tcttcatgaa aactaagtgg
     7981 tacatccaac tcttttgcct taatcattgc ctccataatt agattgctat ccattaaagg
     8041 taatccatca tcagtaaacc ctggtacacc agctttttta agagcttcca tatcaactaa
     8101 ctctttacct tcaaaacctt tagttatagc tgctgcttgt aaaacattta tacatgcatt
     8161 ttttgacttt tctttaacat aattaaaagt atcttcatta tctacaatag gattagtatt
     8221 tgccatacat ataacagttg taaatccacc tctagcagca gatttagctc cactttctat
     8281 atcttcttta tatgtaaacc cagggtctct aaagtgtaca tgtacatcca ctaaacctgg
     8341 agcaactaca ttaccactag catctattat tttttcatat tcatcacttt catcaaactt
     8401 gcctatttta taaattttat tttcttttat aattaagtca atattctcat ctctttgact
     8461 tttagggtct attactctac catttttaat taaaatcata ttatttctcc tttatatttt
     8521 taatttatat agactcatat aatattatac attattttac atacttaaat acaaaattac
     8581 gtttgacatc aatagccttt ttaggacaaa gttcatggca acagtaacat cttatacatt
     8641 tacttaagtc tataatagca cccttagtcc ctatctctat agtggatgca ggacaagctt
     8701 ccttgcattt tccacatttt atgcaatctt gaaatcttac cacaggcttt ggtgttataa
     8761 gctttgaaat aggttcattt aaaaattttg gtagctttac ggaaaacaat cttaaatctt
     8821 tacttacatt tggaatcttg taatttttta taattaaatc ttttatatct tcacctaaaa
     8881 tttcaatatc tgaaaaatct tcttgtatat atcctctctt aatagcacct ctaattgtag
     8941 caactttatt tggaagtaaa cttataatct tacatgcaac aacatctata gcatatggat
     9001 tttgtgatgc taataaaact cctatttctc gagtagtacc agctgaaggt ccatcacctt
     9061 ccatacctac tataccatcc ataattgtca atgttggttt tatataatca caaatatcta
     9121 aaagtacatt ttcgcaaaac aactcttctg taggaaatct ataatgtatc tctgcttttt
     9181 tcaatccagc tatgcatcca tataagtttt ttgtaccacc agtaaaagtt gccattatat
     9241 gagtctttaa tttacaaaga tttattatat aatctgaatc tactattggt ttaataatat
     9301 ccatatattt tagaacatgt gctttttcac tatgtatctt aacatctgaa acatcataat
     9361 ttaacttaat gcccagttca tttgctactt cttcaattcc agatgcctta taaatgctct
     9421 taagagaact ttgattatat ggaccaccag ggctatctgc tattgtaata tcacaattta
     9481 actctaaaag tttttcacat acaactttta caaccatagg atgtgtagtt gtagcttctt
     9541 caggcttttt tttcattaat aaatttggtt ttataagtac tttgcttcct tttttaacaa
     9601 acttatctat accaccaata tcctctaaat ttttttcaat agataacttt acttcttcat
     9661 aattgtaagt tttacaactc ctaacagata ccgacttcat attatctcca tccttctatc
     9721 acaacagttt atttatataa tttaaaaaag gagaaaccaa agtttctcct tttaatctat
     9781 gttttaattg ttttaattat tattttccaa aatgtctgtt taataatcct aagaatgctt
     9841 taccatgtct agcttcgtct ttacacattt catgtactgt atcatgtata gcatctaatc
     9901 ctaattcttt agctctctta gctaatttta attttccatc agttgcacca tactcagcat
     9961 caactctaac tcttaagttt tcttttgtat ctgcaactac aacttctcca agaagttctg
    10021 caaatttagc agcatgttca gcctcttcaa aagctattct cttatacgct tcagctactt
    10081 ctggataacc ttctctatca gcttgtctac tcattgctaa atacattcct acttctgtac
    10141 actcaccagt aaagttagct cttaatcctt cgattatttc tgcatctaca ccttgagcta
    10201 ctcctattct atgttcatca gcccaaacca tttcgccttt catttcttca aatttatcag
    10261 ctccaacttt acatactgga cattgtgctg gtgcagcatc tccttcatgt atatatccac
    10321 atactgtaca aacaaatttt ttcataatta atttcctccc aattttatat aattaaattt
    10381 ttatttttaa gtttaataac ttgctacaag aatatatata ccccagactt tatagcttaa
    10441 acactaaaaa taaaatttat ttcctaatta attttatcac tttgaaacac cagtatcaac
    10501 caagttttga tgtcaaattt ctacataata cataatttat ttgattaatt aggaaatagt
    10561 tgtcatatta aatactttct aatgaagact cctctgaatc tttaagtttc acttttttgc
    10621 tctttaaaaa agataattta gtttctgcag ttatgattgc aacaaatatc aagatactac
    10681 caataaccaa ttttgctgta ataagctcct ttaaaattat tatagaaaat attgtaccaa
    10741 atacagcctc tgtggataat attatagctg ttctagttcc atcaactctt ttttgacata
    10801 tagtctgaaa taaaaatcca attgtagtac taaatacagc tagatacata gttcccatgt
    10861 aaccagataa ttcagcttcc atctttaatt gacctgcaaa tgtttgaact acaacagaca
    10921 ttaaaaacgc tactgtaaat tgaatagctg tcaatgccat tgggttgtta tcttttgcaa
    10981 actcacttgt aaagaatata tgaaatgcaa atccgaatga acatataagt gttagaaaat
    11041 ctccaaaatt aatagagaaa tcagcttcta aagaaagtat cccaattcct ataagagcta
    11101 ccaaactact tattattcct attttatcaa gttttcttct ataaagtata aatcctatga
    11161 atggaactat tacaacatta gctgctgtta taaacgcatt tttagatggt gtagtgtaca
    11221 taagtccaat agtttgtact gtgaatgcta gaaataaaaa tattcctaaa atccctcctg
    11281 cttttaataa ttttttaccc atattggcac gaatctgttt aaaaaacagt aaattcataa
    11341 ttatagctgc caagaaaaat cttaaagtta tgatttgtag aggtgtaagc ccaccgtcta
    11401 atgcaaattg ggttgctaca aagccacttc cccatataat tgctataaat attagtccta
    11461 tttctccaaa gtattttttc aataaagctc cctccttttt atttttttgc aaaattataa
    11521 accccctaaa cagcaagaga gtttttctat aagcattact ataaattaat atagtaaatt
    11581 ttttcagtat tttaaatcta aatttacata cattttgagc tcaaatttat atccactatt
    11641 taacagtaat atattaatca ttttttgtaa acaccttttt aataaatttt tatatttttt
    11701 atattttttt acctatacta aattttttta tagatatttt taatatatat gatactttaa
    11761 tattatgcaa ataatgtcat ataagttaca ctttagttat cttagttaca actttgtcgg
    11821 tttacttttt ctactttctt cgatataatt ataatatatt tttgatacaa ttcgacaaaa
    11881 gggggaatgc tgtaattatg gtaaaaaaaa gaaaaaagaa aactgttaca cttactatac
    11941 ttgttgtaat agttattggt attttttcta tggcatgggc ggacatgtct agaaaaaaag
    12001 aaatgaaaga aactcaagaa agacttaaag ctgaaagatt agaaaaagaa agaattgaaa
    12061 aagaaaaaaa tgaacctatt attggtgcaa aaaaagaagc tgtaaaagaa tatggatatg
    12121 acgcaaaaat agtttgggac aaacttaaca aatacgacta ttcaaacaat ggagaaaaga
    12181 tagtctttct tacatttgat gatggaccat ctactactaa tactccacaa gttctagata
    12241 tcttgaaaag acatggtgtt agaggaactt tctttattaa gggtgattct cttgaaagaa
    12301 aaggagctaa tgaaatttta aaaagaacat ttgacgaagg aaatgcaata gcccatcact
    12361 cttatactca tgactataaa aaactatatc ctaacagaag cctaaatctc gatacattcg
    12421 taaatgaact taacaaaact gatgaagcta tgaaaaaagt cttaggaaag aatttttcct
    12481 caaatgtagt tagatgccca ggcgggtaca tgtcatggaa aaatatggaa cctctaggaa
    12541 actacttaaa agagaaaaac atggcatcaa ttgattggaa cgctttaaat gctgatgcag
    12601 aaggtaaaaa gaaaaatgca caagaattat ttgaacatgc aaagaaatct tctgaaggca
    12661 aagaaatggt tgttctttta atgcatgata cttatggaaa acaagaaaca gtaaatgcat
    12721 tggaccaaat tataacatat tttaaagaca atggttatca atttaaaatt ttaatataat
    12781 aaaaaagcaa gtttattgaa tgtacatttt atactcaata gacttgcttt tactttactc
    12841 tattttacta gctcgatttt tacaaatttt ttctttccta tttgaacaac taattcactc
    12901 tcttttacaa tagtgcttaa atcctctact ttttcaccat ttacctttac tccaccttga
    12961 gaagctaatc ttctaacttc actcttactc ttaacaatct cattagaaac tagtacttcg
    13021 attaagtcaa atccatcttc cttaacttga attgtttgta tatcttcagg tatttgacct
    13081 ttttggaata cagatttaaa tctttcttca gcttctttag cagattcttc tccatggtat
    13141 aatgttacta tttctcttgc taagttcatc tttatatctc ttggatttac tgaaccctct
    13201 ttcaactgag attctatttt attaacttca tcaggatgaa catctgtcac taaattgtag
    13261 tatttaatta taagttcatc aggaatttcc atagattttt ggtacattat tcccgcttct
    13321 tcatctattc caatatagtt acctaaactc ttactcattt tttctttacc atctaatcct
    13381 tctattaaag gcatcattat tactatttga gactccatac caaattctct ttgtaatgat
    13441 cttcccatta gtaaattgaa tctttggtca gttccaccaa gttctatatc agcttctaat
    13501 gctatagagt catatgcttg cattaatggg tagaagaact catgtactga tattggcatt
    13561 tgtccttcat atctcttttt aaaatcttct ctttctaaca ttctcgcaac agtaatagtt
    13621 gctgctaatt ttattacatc ttcaaaattt aacttagcta accactcgct attaaatctt
    13681 acaatagttt tttctttatc taaaactttg aaaatttgtt cttcataagt ttttgcattt
    13741 gcaagaactt gctctgttgt taaagctttt ctagctttag attttccagt tgggtctcca
    13801 atttttcctg taaaatctcc aattattatt actatttgat gacctaaatc ttgtaattgt
    13861 ttcatctttc taagaactac tgtatgccct aaatgtatat ctggagcact tgggtctaga
    13921 cccaatttta ctatcatagg ctttccttct ttttctgatt taataagctt ttcccttaac
    13981 tctttctcat ctattaaatc atctacacct ttcataatta ttcgcatttg ctcgtctatc
    14041 gatttcattt ttcattcctc ctaaaatatt tatataatct ataattttta tttttataat
    14101 aaaaaaagct ttcatcctct taaaaggacg aaagcattgc ttacgtgtta ccaccttagt
    14161 tcatcaatat ctcacgatat taatctctta gagtacaatt acatatactc gtgtactata
    14221 acgtgtacta atccgatatc tcttaatggt tgttatcttt cagagatagt gctctgagat
    14281 gtattcaaac taaatcaatt tattccattt caccaaactg gaactctctg ttaaataaaa
    14341 tctagcccta cttcttctct tcatagcatt tatatattca gattatttca tcaattacaa
    14401 gaaatttttc ttaacttact cataatatat tattattttt atattttgtc aacctttttt
    14461 attattatat acatgttttt ttacattata ctactttttt atatttttta ccaataccca
    14521 aaatttattt ttcaatcaaa agatttattt gtttattgac tatattactc tgactcaatc
    14581 ttatatttat tttcttattt aaaaatgcgt ttataattat taaactaata actgttacat
    14641 caaatataaa aaacagcaat aaaattatta tcaaaaataa aattctagca tataataatt
    14701 ttctaaactt agctattata aagttttgta gtgacataca tgagtctcta atgataaaat
    14761 agacacttat ataggttatt gatataaaga ttgtttttaa ataaacagaa tcaactttaa
    14821 cattcaacag ttttagaact tcaaaaatgg ctagataaga aacacatatg taaaaagctt
    14881 tttttgagca acataaaaat tggtttaaat ttctaaagtt cttaaatatt aaaaattttt
    14941 ttaaagaatt agaatctaaa atatgcaatt tattccctaa aattaaactt aataaatata
    15001 tgcaaaacac cataccaaaa ggaaacaatg ctatcattaa ataataaata aaatttgtat
    15061 acccattaaa ataaatccat ttgctatatc cttgaaattc tttcataaaa ctataaccta
    15121 ttaatgttga agcatatata tcaatgaaaa ccattacaat aaccccaaat actgaaccat
    15181 atactaaatc atccataacc attgtaggct tatttattag atagccacac attataccaa
    15241 gtaatacact ctgacttgcc caaatagcag ttcctagatt tccaagtacc agagaaatta
    15301 ttaaaagcga agtaattcca gataaaatgg tatactttaa atcacatttc aagcagatta
    15361 tacaaaaaaa tactggcact ataaaatcta agtaaatagc atatccaaat ccagtactca
    15421 cagctataat tgttactact atgaataaag atgacaataa agctgcttca gttaattttt
    15481 tagtagtatt caaaacactg cctccataaa tcataaattt tctaactaaa gttttgtttt
    15541 taatatagta tatcactttt taaaaatatt tttaataaat ataaatttta ataaaggtat
    15601 ttatgttaaa aatggtataa tgtatcttaa tattcagttt tttaagatgc taggaggtaa
    15661 ttactatgtt taaaattaaa cactttaatg atttgagttt agatgaattt tatgaaatag
    15721 caaaatcaag atatgaggta tttgcttgtg aacagaagat attttcatta aacgattatg
    15781 atgatataga taaaagctct tatcacatat ttttaaaaga aaatggattg atttgtgctt
    15841 acgctagaat aattcctaaa gaatacagct cttataatga tgtttccatt ggtagagttt
    15901 tagttctttc tagtcataga agaaaaggat tagcaaaaca aatgatggat tgtgctatag
    15961 attttataaa agttaactta cacgaaaata atataacctt atcagcacaa acttatataa
    16021 agaaccttta cttatcttgt ggttttaaag aaatctcaga agtatacgac gaagcaggta
    16081 ttgaacatat taaaatgaga ttatagtgtt gacaagctaa tattgttaat atataattta
    16141 agtataaatt aaatattgaa ttgatattta agtatttgaa aataccttac atagagtcag
    16201 aaggcttttg ctttctgact tttttattat caaaattcaa taattatatg gattcaaagt
    16261 gttaattttt attctatatt ctactttccc caaaagtaag aattataccc aactttgtat
    16321 gataggcatc ttgcctatct tttttttgta aaagtaacaa ggtaataata taatgcatag
    16381 tttaaaataa gaagattagc ccaggctaaa tttcaagcat ggaggtgcta ttatgaaaaa
    16441 ccttaacgat attcaagtga aaaaaactgt caaagttgaa gatatattat ctagtggtaa
    16501 tttaagagaa agaatgttag cgcttggtct tactagagga gctgttatag atgtagttag
    16561 aaaaggtcca aaaaataatt taactgtcta taaaatcaga ggtagcaaga tagctctacg
    16621 acaagaagaa agtagcttaa tattagtttc tgacatttaa aaatttctag caggaggata
    16681 ttaatatggg gttgacacat aactcaacta aaatgagttc tttaaaagac atgtttgata
    16741 tagacaataa ggaagaccag tttgtaattg cattggctgg caatccaaat acaggtaaaa
    16801 gtactgtatt taatcatctt acaggactta gacagcatac tggaaactgg ccaggaaaaa
    16861 ctgtagctac agctcgtggt aattttaaat ataaaaatac cgaatatgca ttaatagatt
    16921 taccaggaac atattcttta tttgccttat cacaagaaga aattgtagct cgtgatttta
    16981 tatgttttgg taatccagat gcagtaatag tagtatgtga tgctacatgt cttgaaagaa
    17041 atttaaattt agtatttcaa gtgatggaac ttacagataa agttattttg tgtattaatt
    17101 taatagatga agctagaaaa aaaggcatta ctattgataa aaaactttta gaggattctc
    17161 ttggtattcc agttatttta actgctgcaa gaaatggctc tggtatggat gaactattgg
    17221 atactttaaa tgatgtatct tttgacaaat ataaattaaa taataaacct gttagataca
    17281 atgaaaatat agagaatgtg gtaaaatcaa ttcaaccaga attggataat attataccag
    17341 gtataaattc tagatggttg ggacttagac ttattgatgg cgatgaaagt atatttgaat
    17401 ctatgagtaa ttatatagac aaagattcta tagatgctat taatgaagta aagaaaaaga
    17461 ttccagacaa tataaataaa cagaaaataa gagatgagtt tactaaaatc aactatgatt
    17521 atgctaaaaa actgagtgat gagtgttgtt ctaatgttgc taaaaaatca actgatagag
    17581 aagaaaaagt agataaaata ttaacatcta aaatatttgg gcttccaata atgctcttat
    17641 tattaggtac tatattgtgg attactatag aaggagctaa ctatccatcc actttattat
    17701 ctaatttatt actcggattt gagccaagta tttcgggtat attaaatagt attaattgtc
    17761 catcatggtt aaatgatatg ttggttttag gattatacag aacattagca tgggtaatct
    17821 ctgtaatgct acctcctatg gcaatatttt ttccattatt taccctactt gaggattttg
    17881 gatatttgcc aagagttgca tttaacttag accacctatt taaaaaagct tgtgcacatg
    17941 gaaaacagtg tttgactatg tgtatgggat ttggatgtaa tgcagctgga gttattggtt
    18001 gtaggataat tgactctcca agggaaagac ttatagctat acttacaaac aactttgttc
    18061 cttgtaatgg tcgttttcct actttgatag ctatatctac aatatttttt agctcagtta
    18121 taaccaactc tttcgtatca agcgtagcaa ctgcactttg cataacttta ctgataattc
    18181 taggtgttat aataacattg ttggtttctt atactctttc taaaacgcta ttaaaaggag
    18241 ttccgtctac atttacatta gaacttcctc cttatagagt tcctcaaata ggaagaactt
    18301 tatatacctc tataatagat agaacaatat ttgttttagg aagggctgta atggtagcca
    18361 tacctgctgg tgtcataact tggatatttg caaatatata tataggagat ttaagtatat
    18421 tatcacatgt tgcaaacttt ttagaccctt tagctaaatt aattggtctt gatggtttta
    18481 tattacttgc ttttattctt ggattcccag caaatgagat agttgtacct atacttttaa
    18541 tggcttacct agcaactggt tctatgatag aattagatag ctttagtgct ttaggtcaag
    18601 ttcttagaga acatggatgg acttatttga cagctttaaa tgttatgctg ttcagtttac
    18661 tgcattggcc ttgtgcaaca acacttttaa caataaaaaa agaaactggt agtttaaaat
    18721 ggactgcctt aggattctta atgcctacta tacttgcttt tgttgtttgt tttttaacaa
    18781 ctacagttta caatttattt atataattag tattaactga cagtagacat atattagaga
    18841 aaccatttaa aaatttatat actttattat aaagcataga gatatattca atgataagta
    18901 aaattactta acatttgaat atatctctat tttaattctt atctggatac ttggattgaa
    18961 atttcagtca tataattatc ttcattactt tcacaaaaaa agtcaagtat actctcctca
    19021 tacgaatttc caacaatttt atattcattt tcatatatat attttttcaa tttttcgtaa
    19081 gatttatatg aatcctcata ttttccaata tgttttatgc aagcatacat tccttctggc
    19141 tttacatata gctttttatc ctttattttt ttatctatct tcaaataaaa acttgatata
    19201 cttaaatagt ccccattttt tatattttca ttacttacta ttactcccaa ctcatatcct
    19261 tgatacaaat ttttcgattt acaataattg gtaaaatcta ttaattccat cataaaatca
    19321 ctattactca tattactcaa atcgcttgat agaactaaat attcttcaaa ctttttggat
    19381 atataaattt cttcactatt cattttagaa attccttcaa ctgttatatt tattctattt
    19441 tgcatcttag aacttatctt tcttagtttt tttatttttt ctcttatctt tttttcttct
    19501 tctttaagca ttttcacaca aagctgggga gttctatttt gtatatactc ttttatttcc
    19561 tctaaactca ttcctatttc tcttaatgat tgtaatatat caagtgtatc atattgataa
    19621 actgagtaat atctataatt atttttatct ttatattcag gagaaaaaat acctattttg
    19681 tcatagaaaa ttaatgtctg cttagatata ccacacaatt tagcaaattc acctgttgta
    19741 aaatactgtt tttccaaggg ttatacactc cttttctttt tgttgtctat atagttacta
    19801 tattctttat tatttactaa atcaagtagt tattatttta gaaaatcaat atggttttga
    19861 ttttaaagct taaatatcag ttagatgtca gttataaata ttttacccat tcccatcata
    19921 aaaaagatat cccctcttaa cttttggatg gaatattata tttatacttg ttagagtgaa
    19981 aattgtaata ggtgctacaa taagatattt aattaaacat aaagttaatt attgtaataa
    20041 aaaatcttta gataaattag agttaaagta tggaaatata gatagagaaa aagctgttaa
    20101 actagaaata ttttatcaat atttaataga acttgaatat atagaaatga gtttattaat
    20161 aagaagatta gatactgcaa taacatcatt agcattagta tctattataa caatagcatc
    20221 atattattta atcagaaaaa atgtgtaatt atgtaagcta tttataataa aacactgata
    20281 aaattaataa taaaaagata ataggatttc tatactacct tttgttttta taatatagtg
    20341 taattttatt atatttttgt atgctaatat aactagataa tcataagata acacattaaa
    20401 agaaaattat attctgtata gttagtataa aacgtaagta atataaattt atgtaaagca
    20461 acaaaagatg gatttacatt ccatatagtt aatataaaac gatttcaaat gattagaata
    20521 aaaatatcag aattgctatt tatattccat atagttaata taaaactata aagtgttact
    20581 tttatttatt ttatttttta cataatttac attccatata gttaatataa aacgcattat
    20641 atggctgtgt cattttgcat tctattacat atttacattc catatagtta atataaaacc
    20701 tgcttaatgc agaattaaga gaggaagtgt tataagattt acattccata tagttaatat
    20761 aaaactggtt gttcctgctc cggtgttggt ggaactggta catttacatt ccatatagtt
    20821 aatataaaac gaatatgtat ggataatttt ttacttaaca tattagcatt tacattccat
    20881 atagttaata taaaacctgc tgtaatttct gatgaagctt ctccactacc accatttaca
    20941 ttccatatag ttaatataaa acccttcttt aactcttatc gtatttaaac ctcttataac
    21001 atttacattc catatagtta atataaaact ttttgtgtta agaagaaaaa agaaattttg
    21061 ataatattta cattccatat agttaatata aaaccttaaa gaaaaaatgg aggaagatta
    21121 agtgaaaaaa ttatttacat tccatatagt taatataaaa ctggatttaa ataatcaaaa
    21181 caactccggt ttaaaaaaca tttacattcc atatagttaa tataaaaccc caaaataaac
    21241 ttagtatttt caacacttac acgtatacaa ctctcttaaa tttgcagtga acgagcaata
    21301 gtgcaattga taacgcttat catacactct caacgcctta tatttcaact gttacgccaa
    21361 attttttcac aaatatcgct cactgcaaaa ttcctataat tttattatat cataaatata
    21421 ttacttttga atattgatac caatttatac cataataaaa gcaaaaaaac gtaatctatc
    21481 tacattagat ttaaattcta taacttaaag ttttttacat aatatttatg cttgtttaat
    21541 caatctaata tatcattctt gtatattgct tttaatagtc ttttttctta aataaatatc
    21601 tatctgaaca agcatagttt aattaaaaac ttttgtattc ccttaaaact gtatctacct
    21661 cttattattt tatttatgat atattatttt taatctaaaa atagatatct atttttatta
    21721 tggttatccc tttttaaaat actttaatca tatactattc ccaatttaac atccattgaa
    21781 atgctatttt cttatattgc aatttataat tgatatatct tcaatataat aatttaatgt
    21841 atttttgatt ttaaagcttt tcagtgatat tattgataaa ttagaattaa ttatctagct
    21901 ttaaaaggtc aaagatttct gttgctgtct gcggttttcc aaataaatat ccttgtatgt
    21961 aatctggctt tatttgttta actaaatcat actcttcttg tgtttctaca ccttctaaac
    22021 atacttttat tcctacatca tgacaaattg caacaataaa atgtataaat gtagcatcaa
    22081 acttactctt taatatatct ttaacaaata ctctatcaat tttcactata tctattggag
    22141 aaaattttaa aacttctaga gacgagtatc cagtaccaaa atcatccata gcaatataaa
    22201 ttcctaaatc ttgtaaagcc ttaaatttaa actgtaaaat actcatattt tgtactgtat
    22261 gactttctgt caattctaaa acaagatttt gataaggaaa attttcatca tctataattt
    22321 ttactatatc ttcaataaat gtgtcttcta atatttgaac agctgaaacg tttacactca
    22381 ctttaaaaaa aggatagtaa tcaatccatt tcctaaaagt acgtaatgcc attcgaagca
    22441 cccaagcccc tacaatattt atcatatcat tttcttctaa tattggaata aattccaaag
    22501 gagaaattgc tttgcatttg tcattagtcc accttaaaag aacttctaca cctattattt
    22561 tatgactttc tgtatctact tgtggctgga atcgaagact aaacccccta aaatcatcat
    22621 taattgatgc ctttaaatcc cgcatcattt ctaatgaata cattttattt tttaatattt
    22681 cttgggagaa aaaaacaagc ctgtttttac catgctcttt cgcatattgt aaagagtaac
    22741 tagcacactt ataaagttct tttactgtat ctccatgttt tggatatatt acacaaccag
    22801 cagaaattgt tatatttaat ccatattttc tccatagttg aaggtgtata atcatattct
    22861 gtatttgatt atatagagtt agaatttcat tttcttcaac attatcaaca agaatgccca
    22921 tttcgtcccc atcaagttta taaagttcag cattatctgg taaaatagat tgtatagact
    22981 gtgctagtgt ttttattata ttatctccaa aatccctatc atacatctca ttaacatttt
    23041 taaaatcgtc aatatctaat aatactatgc ataatttttc aatcatagga tttgaaattt
    23101 tatcttcaaa tacactcata aattcatgat aattcagtaa ctgtgtcaat gaatcaatct
    23161 tattttgttt acctaattgc gtcattattc ctgcaaaaat acttggttct ccaaattcat
    23221 cacgcattag ttgacctcta catcttaacc atatatattc tccactacga tttttagccc
    23281 taaattccac tgtatgataa tccatctggt ttttacctat ttcagtgttt gacttataaa
    23341 aacgattcca gtcttctgga tgtacaattt ttttccagta aacaagcgga ttttcaacaa
    23401 tttctcctgg taagtcaaat aattcaactt gtgatggtga atatcgaaat acgcctgttt
    23461 tcatattgca aatgtatata tattcatctg tacctctaat caaagcatca taaagcaatg
    23521 tagaattata atcaaaattg tctcttgaaa aattaaaaac tcctttttct tctttattat
    23581 ttgatatttc atgaaattca ttaatcttaa ttggtttatt taagcttgtt gagtttgcaa
    23641 tatctattga ttgatgcata tgaacaactg tgccatcttg ccatgtaatt aaactatcat
    23701 aattttcaaa aacacgattt aacttattac atttttcata ccactttatt aatactcctt
    23761 ttttatcatt ctttaataat tctaaaactt tacaaaaact acaagtagaa tttttttcag
    23821 gatataatac ttgccaacaa acttttcctt ctggatctaa aatattatat tcttctttca
    23881 tttttttgtt cataaaaata atttcattag tatgaatatt tgtaacatat atattaatct
    23941 gtagttgatt taatataact tcaaaattat gtttgttcat atacttatct cctccatagt
    24001 tactgctctt tataacaaca ttgttaaaat tattcttttt agaagattta cttataactt
    24061 atacatatac actaaatacc ttaatttaat tagaaag
//
LOCUS       DBGJFU010000054        23644 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.054, whole genome shotgun sequence.
ACCESSION   DBGJFU010000054 DBGJFU010000000
VERSION     DBGJFU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.054"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            262..2280
                     /locus_tag="ACOIAS_002022"
     CDS             262..2280
                     /locus_tag="ACOIAS_002022"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2701734.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            2788..4800
                     /locus_tag="ACOIAS_002023"
     CDS             2788..4800
                     /locus_tag="ACOIAS_002023"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2701735.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5042..6955
                     /locus_tag="ACOIAS_002024"
     CDS             5042..6955
                     /locus_tag="ACOIAS_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701736.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKVTTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            6948..9167
                     /locus_tag="ACOIAS_002025"
     CDS             6948..9167
                     /locus_tag="ACOIAS_002025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGZ2701737.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKSGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLSLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9276..10106
                     /locus_tag="ACOIAS_002026"
     CDS             9276..10106
                     /locus_tag="ACOIAS_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HGZ2701738.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10096..13563
                     /gene="addB"
                     /locus_tag="ACOIAS_002027"
     CDS             10096..13563
                     /gene="addB"
                     /locus_tag="ACOIAS_002027"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HGZ2701739.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13563..17390
                     /gene="addA"
                     /locus_tag="ACOIAS_002028"
     CDS             13563..17390
                     /gene="addA"
                     /locus_tag="ACOIAS_002028"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HGZ2701740.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFSIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKSDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17436..18647
                     /locus_tag="ACOIAS_002029"
     CDS             17436..18647
                     /locus_tag="ACOIAS_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HGZ2701741.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18634..22161
                     /locus_tag="ACOIAS_002030"
     CDS             18634..22161
                     /locus_tag="ACOIAS_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2701742.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22292..23545
                     /locus_tag="ACOIAS_002031"
     CDS             22292..23545
                     /locus_tag="ACOIAS_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGZ2701743.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT        10197 a         2289 c         4140 g         7018 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattgt tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tgtacttatt tataattttg
      181 ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa gtagaaattt
      241 atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac agggattctt
      301 tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaac tacaatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacacaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgcgc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag ggatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtgtaca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg ccctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagc tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgccaa cttaaaatct ccaatactta taacaccaaa gacgtatgta
     1561 tctgcatacc ataaagaaaa cttagaagct aaatcggcta ataaggtata taaaatagga
     1621 ggaggattga cttctaaggt aatgaactct atagcatcat cattatctaa acacaatacg
     1681 actccaacag aaccaggaaa tagtgggggt aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttaaagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagata cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggaggaacta ctaagactgt agctacaaat
     2041 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tataatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataaatgcga aatgaggtat atacagtctt tttataatta attgtaaaat gatttatgtt
     2461 atatgaaaaa tttacatata ttaaggctac agttaattgt caagagctgg agtattcatt
     2521 tgtaatacac aatataagct taattactat attttattgt gtgttttttg tggattttta
     2581 tgtcaataaa tgatatagta tagataatta aatgtattaa atatatttaa tttttttatt
     2641 ttttgagttt tataagttta tttttatata ttacttatat attacttata tattatttat
     2701 attttgggtt agatacactt tgacatattt tcaaaagtta aaagattaaa aataaagtag
     2761 aaatttatgt taagtaagga gattaatatg agaagaaata caaaattatt aacaacaggg
     2821 attctttcaa tggcaatcgt cgcacctaca atggcatttg ctactgaatc taatgctatg
     2881 gaaaataacg ctgatttaaa tataaactta gagaaaaaaa gtatcgtttt aggtagcaaa
     2941 tcaaaagtta gtgtcaaatt taaagaaaaa ccagatgcag atagcattac attaaagtat
     3001 aaatgctatg acatgccatt ggatacaact ctaaattaca atcaatcaac tggggcatat
     3061 gaaggaacta tcaattataa ccaagaccca gaatatctaa atgtttggga actacaaggg
     3121 ataacaataa acagcaaaaa taatcctaaa actttaaaca gacaagacct agaaaagctg
     3181 ggattaaatt taaaagacta taatgtaaca caggaatgta taattgaaga cataacttct
     3241 agaaaagatg taaataaata tttgagaaaa acttcttcac ctattacaga acttacagga
     3301 agtgatagat atgaaacagc agttaaaata agtaaagagg gttggaaaaa tggttcagat
     3361 aaagtagtta taataaatgg ggatgtaagt atagatggca ttatatcaac tccactggca
     3421 accacatata atgctccaat acttttggtt gaaaaaaaca atgtgcctaa tagtgtaaaa
     3481 tcagaattaa agcgcctaaa ccctaaagat ataattataa ttggagatga gaatgctatt
     3541 tctaaaacta ctgctaatca aattaaatca actgtaaatg ctagtcaaac acgtttaaat
     3601 ggttctaata gatatgagac atctttattg atagcaaagg aaatagataa aaatcatgat
     3661 gtggaaaaaa tatacataac aaatgctaat ggcggagaag tggatgcact tactatagca
     3721 gcaaaagcag gtcaagacaa gcaaccaatt atattaactg ataaagatag tattacagac
     3781 aatacatata aatggttaaa gagtgaggat ttacaaaatg cttattttat aggtggtcct
     3841 caaatgatat caacaaatgt tataaataag gtaaatggaa taactaagga tagtgttact
     3901 aataatagag tatacggagc agatagacac gaaacaaatg caaacgtaat aaaaaaattc
     3961 tatacagatg atgagttaga ggctgtttta gtagctaaat cagatgtact tgttgatgct
     4021 ttagcagcag gtccattggc tgcgaactta aaatctccaa tacttataac accaaagacg
     4081 tatgtatctg cataccataa agataattta gaagctaaat cagctaataa agtatacaaa
     4141 ataggaggag gattgacttc taaggtaatg agctctatag catcatcatt atctaaacac
     4201 aatacgactc caacagaacc aggaaatagt gggggcaaga cagttatgat tgacccaggg
     4261 catggtggtt cagcacctgg aaattcatct ggaggaatga ttgaaaaaga ttacaattta
     4321 aatacttcac ttgcaacaac tgaatattta cgttcaaagg gattcaatgt aataatgaca
     4381 agagacacag ataagacttt atctcttgga aatagaactg ctctatctaa ttcattgaaa
     4441 ccagatttat ttacaagtat acattataat ggctcaacta ataaacaagg tcatggtgta
     4501 gaagtatttt ataagcttaa agataaaaat ggagggacta ctaaaactgt agctaccaat
     4561 atattaaata gaattttaga gaaatttaaa cttacaaata gaggtataaa aacaagagta
     4621 cttcctagtg attctacaaa agattattta tacgttttaa gaagtaatga tatgccagct
     4681 gtacttgtag aatgtgcatt tttggataat gaaaatgata tgagtttaat aaactcatct
     4741 gcaaaagtaa aagaaatggg tacacaaata ggtaaaggaa tagaagattc attaaaataa
     4801 atggaaataa aaatatttaa aaacttaatg taaatttata gttgtaaatt tatactaaag
     4861 tcaaaaaatg aaatataagt gtatcaatag taagactgta tgtacatttg cacatgtgaa
     4921 tgcaagtata tatacagtct ttttttgtag ttaaatgcaa taatttgatt gtgttttgta
     4981 aaatacacaa tatgatataa ttagataata tacacttaat ttaattgagg aaggtgatta
     5041 tatggaatat ttatttgaaa aaagtagttt aaaagaatat ggaaatgaag ctttagataa
     5101 atatagagaa ttattagata aaactggtaa taattcaagg tgtattcttt tgttagtacc
     5161 aaacaataac acaaagatac gatatgaaag ggctctaaga ttaaattata gtgaagaact
     5221 gaaagtaaca acatatataa gttttgtgaa gaaagaatta gttaaatact ggccactaat
     5281 aatagaaaaa tgtgatggaa taagtacaaa ggttgtatct ccaacattta tatcaaatag
     5341 tttaagtgaa tatttaatag tgcaaaaagt taaggaaaaa aggatacaag aaggatattt
     5401 tgaagatatt acaggaacca ataaatccat agcaaatagt ataatgacta acataaataa
     5461 gtctgcattt aatttaattg attttgattt tattggagaa aaaatatatt catcaaagaa
     5521 aaataaagat agtatatata gattttcata cactcaaatg gatgaaatta tatcatatta
     5581 tataaattcc ttactatcaa attcaatttt agataatgca ttaagcatat atttatacaa
     5641 tcaataccta cttaatgatg atttttattt aaaaaactta tttacagaag tgagatatat
     5701 aattgtggat agccttgaaa gctcaagtaa tgcagaagtt gattttataa cagaagcatt
     5761 ggataataca ttacaaagtt atatatattt tgattatagt agggattatt ctgtatttaa
     5821 taatgtggat atagactata taaatgagaa aataatatca aaaattaagc ctaaagaaga
     5881 aaatattaaa aagaaggaga ttagaataga agatttatac ttattacctg caaatataga
     5941 attaaatcaa tcaagtcaat tatataatga aatgttagat ttaataagtg aaaaagttat
     6001 atctcttata gaagaagatg tatctccaag agatatagca attatatctc ctataaacaa
     6061 tagtatattg gagcatcaaa ttagagattc tttgatagaa aaaaatatag atgtttttag
     6121 tactaagaag gataaaaaag ctgtggatta tccatatggg aatgcattgg tagtggctac
     6181 atgtatattc tatggatatt tagattttat aaaagatgat gaatacttaa gctttttaga
     6241 aactctttta gaaataaata ggataaaagc atttaagatt tttaatgaaa caagacattt
     6301 aagtattgat aatatagatg aacatagtca gtatcgtgat attttacagt atattgaaga
     6361 aaaaaagaag agtgatataa aaatacatga atttttaact caattttaca tagataaaat
     6421 gttaaattta aaagaaggta aaaagaatgt tgggttatgt aaaaagataa tagctgaaag
     6481 tgagtctttt agtgaaagtt taagtctact tgggatgaaa aaagaaaaga tatttgtaga
     6541 agctttaaaa agtacaataa acgattatta ctctgttgtc gatatagaag agttgaaatc
     6601 caaagacaaa atagtaataa ctactcctta ttcatatata tcatcaaata tagatagaag
     6661 tatacaaata tgggtagata taggaagtaa tgcttggaat atgaaaatag aaaaagatat
     6721 aagtaattta attgtactta gaaaatcttt tgaagaaaaa aagatttata caaatgaaat
     6781 ggaagaatat tataaaaaat attatctata caatacagta tataatttgt tattaaatgc
     6841 aaagaaaatc tatgcattta aaagtgaata tgcgattaat ggttatatcc aagagagtat
     6901 attatatagt atacttttaa aaatctcaca taaaggagat aaaaattatg attaatataa
     6961 attatagaga agaccagata ccaataataa attacgataa tggtactatg gcagtaccag
     7021 cagtaccagg tgctggaaaa acatttataa taacaaattt agtagctaaa ctattacttg
     7081 aacagaaaca taagagtggt aaaatattaa ttcttacata tatgaatagt gctgtaaata
     7141 attttaaagg tagaataaga aagattcttg aagagaataa aatagatgat actaattcgt
     7201 atgaagtaat gactatacac agtttggctg taaaaataat aaaggaaaag ccagaagttg
     7261 taatgttaag tgaggatttt aacatagctg acgatttgca aaaaagtatt atattaaatg
     7321 aatgtataag tagatttaga tttgaaggtg gagaaagagc ttttagatgg tttttaaagg
     7381 agcaaaaaga tgaacgctgg aaagaaataa ctcttgaagc ctgggaaaga ggtttttttg
     7441 aatttgcagg aaatgcaata agtgaactta agtataaggg aatttctcca gataaacttg
     7501 aagaaatatt gtcaagagga cacaaaggaa tgcttaaaat tattttacca atatataaat
     7561 tgtatgatag aaaattaaag caaaatggat tgctagatta tgatgatata ttaattttag
     7621 cagaaaaaac tttaagctta gacgaagggc ttagaaagaa gtttcaaact agatacaaat
     7681 atatatttga agatgagtgt caggactcaa atgaaattca aggtaatata ataaaaatta
     7741 tatctagcga aaataaaaac ctagttagag ttggagatat aaatcagagt ataacaggaa
     7801 cattttcatc atcagaccct aaatttttta aagaatttat aaatagtgca gatttctgtt
     7861 ataggatgga tatgtcaaat cgaagttcaa aagatatact tgatttagca aacactcttg
     7921 tcaagtatgt tacaaaggaa tttagacaag aagaatgtag acatgctctt gaagatatgc
     7981 aaataaaaac tgtgccaaat ggaatgggat ataaagaaaa ccctaatcca gaacattata
     8041 atataaatgt aaagtggtat gagagttgga aaattgaaat agagcaaact gctagatacg
     8101 ttaagggtat aaaaaataaa tatccagata aaagtatagg tatattggtt ccatttaatg
     8161 aacaagtgac acaagttgct aaagaactta gagagaataa tctaaccttt gaagaattag
     8221 gaccaaattc tttgaataaa aggaaggtta taaacaacat agcatatatt attgatttta
     8281 tgttacattg tgatgatgta gaaaagttaa tagttgtact taataaagtt tttatagata
     8341 ctgataatga agaagaaata aagtattttt tagaagaatt aaaaaactat tctaccgaag
     8401 aaattattta taattttgaa tttagtaagt tagataatga tttaatagaa tttaaagaag
     8461 aaagtgatat atacttaagt tttaaaagag gaatagatat tataaaacag atattagaat
     8521 atccaataat aaggttagac ctcttagttt tgtttattgg gaaaaaactt agtttagaaa
     8581 aagaagataa agctgtactg gattatatat ctttttatat aaagtatcta gtatcagaaa
     8641 atataaatat ggatttagaa gatgtatata atttattgtt tgatgtaaag aacagggtgt
     8701 ttaatcatat aatagatgtg gtatatgaga taaatggata tgaaccagag cctggtagta
     8761 taacactatg taactatcat aaatcaaagg ggatggaatg ggattgtgtg tttttactag
     8821 gtcttgttga atataatttc cctgacaata ttaatcaaaa gtttcagagt gataaatggt
     8881 atctaaaaga aaaatataaa aaccctatgg caattattaa atcagaagtt gaagctatat
     8941 taaaaggaag tatttcaaca gattatgctc ataaaacgaa gattgattct ataaatgaaa
     9001 agattagact tttatatgta gggattacta gagcaaagga aatgttagta ctttcaggaa
     9061 gtgcttatag agatgaaagt gatataggga ataagagaaa agagcaaaaa ccatgtatat
     9121 atttaagcag acttaatcaa catattattg aaaaaaggtc aaattaatat ataataagtt
     9181 attaaaagat tgtttgaaat atatctaaaa gaggataaaa caatatgtac agtaagtaac
     9241 tactaaaaaa taaagttaag agagaagtga tatgagtgaa cgataaatta aaatattttt
     9301 cttatagtca aaattcaatt aacacatata aatcatgtcc attgaaattt aaatataaat
     9361 atattgatag aataaattgg aaaaatgatg atgttggtag tagagagtat tatgaaactt
     9421 taaaaacagg aagagatttt catttgattt gtgaaagata ttttagcaat atacctcttg
     9481 gaatctattt taatgaggat gataaaaata gtaaaaagtt tttaaagtgg atggaaaata
     9541 taaaaaaagt tgttcctatt gaaaaaggaa aaacgtattt gccagaatat gaagtaagga
     9601 tgacattaaa tggggatata atacaggcaa agtatgattt agttgtaatt ggaaataaca
     9661 atatagaaat atgggattgg aaaaccgagt ctaaaaagat tgactataaa cacgtagaaa
     9721 atagaataca aactattgtt tatatgtttt tagcaaaaga agtaatacct aaagttttaa
     9781 aaatggacat aaatgttaaa gatataaata tgagatatta tcaacctgaa tttgatgact
     9841 taccaattac cattttatat gatgaagaaa aacatgaagc aaatagaaat aaaattcaaa
     9901 attatataag tatgataaaa aacactaatt atgaagaaca tacatacgaa gacaatctat
     9961 acaatgatat agaaaaggta tataataaag aagaacgtat gtgctataga aataaaaagc
    10021 attgcaagta ttgtgaattt aataagttat gtaatggcaa agaaatagat tatagtattt
    10081 tggaggcgga aatttatggg acttaggttt gtattaggta gaagtggaag tggaaaatcc
    10141 acctatatac tagatgagat taaaaaagaa gctcaaaaaa atgaaacgac atcaattata
    10201 cttttagttc cagaacagta cacatttgag gctgaaaata gagtaagtaa actattttta
    10261 ggaaaagaaa aagataagta tctaagagtt agggtactta gctttaagac actaagtaat
    10321 atagtttttt cacaagtagg aggattgaca gatgtaaata taaattctag tggaaaagca
    10381 atgatggtat atagagctat tgaagatgta agtgaagaat taaatgtatt ttcaaaatca
    10441 aaatcgcagt ctggttttgt aagctcaatt acagatatga tatcagaaat gaaacaatac
    10501 aatataagcc ctgaaatgtt agaaaatata tctggagaat tggataatga aactttaagt
    10561 ttaaaattaa aagatataag taaaatatat aattcttttg aagggaaatt gcatgaaaat
    10621 tatgtagatg ctcaagatat gttaacttca ttggcaagta aaattgaatt aagcagttat
    10681 ttagatggag cttgtgttta tatagatgaa tttacaggtt ttacaccaaa tcagtacaat
    10741 gtcataaaga gtatattaaa taagtctaaa agtgttaata tctctttgac tgtagatgat
    10801 atcaactata ttgggtacag taaatctgat atgttttcca gaacgaaatt tacttattca
    10861 aaattgactc aattgtgcaa cgaagaaggc ataaaaatat tgccacaagt aaatttaaac
    10921 acaggtgtta taaaaagatt tgaaaaagtt aaggaattac agcacttaga aagattttat
    10981 aatgcttatc catataaaat atattcaaat caaactgaaa atataaagat aaaagagttt
    11041 aacaatttat attcagaggt ggaagagata gccagagaaa ttgtacattt agttagagat
    11101 aagaatgtca gatatagaga tataaccatt gctacaagag atttaaatag atatgatttt
    11161 ttagtacact ctatatttaa tgaatataat ataccaaact ttatagataa gaagagagag
    11221 gcaaaaagta atccaatagt tattttaatt atttcagctt tagaaatgaa gaatagacga
    11281 tatgggtatg aaactatgtt taggtattta aaaagtggac ttataggaat tgataatgat
    11341 gatataaatt tactagaaaa ttatgtgttg gctaatggga ttaaaggtaa aaaatggttt
    11401 gatgaaaaat gggattatag aataacccaa agtctttcag gtcaagaaag tgaatttgaa
    11461 ctggaattaa aagagaaaat aaatgaaata aaaaatagag tattagagcc tatagtaatt
    11521 ttacaagaaa aattaagagg aaaaaataga gtaaaagaaa tctgtagata tatatatgaa
    11581 tttttattag atataaatat gccagaaact atagaaagtt taataataaa ttttaaggat
    11641 aaaggggaac ttgatgttgc aaatcaatac tcacaagttt gggacatagt agtggacata
    11701 ttagaccaaa tggtggaact tatgggtgat gaaataattt ctttagaaaa atttataaaa
    11761 ttaataacct taggttttga tgaatatgaa ttaggactgg ttcctcctag tatagaccag
    11821 gtacttgtaa gtagtgtgga tagaatgaaa aatccagaca cgaaatattt atacctagtt
    11881 ggaactactg atggtgtgtt tccgctgatt acaaaggata gtggaatatt gagtgataat
    11941 gatagggaaa gtcttggaaa caaaggaatc gaagttgata ttgatagtaa gactaggagt
    12001 tttgaagaac agtttcttgt atataaagct cttacttcga catctaagaa tttaaccata
    12061 acatatccaa tatcagacca tgaaggtaaa acgcttagac catcaataat tatatcaaga
    12121 cttaagaaga tatttccaaa tatagaaaat aaaagttatt tagtggaaga aaataaaagc
    12181 actgataaag atatacttaa aaaaataacg gttaaatccc ctacatttaa tgaacttata
    12241 aacgtaataa aaaattatga cagtgatggt tacaatacag aagagattaa tagtatttgg
    12301 ttagatatat ataggtacta tttaaaagat gaaatctaca gttctataac aaaaaaagtt
    12361 ataaaaggtc ttagttatac taatcaagtt cataagattg aagagaaaaa aataaggtca
    12421 ttgtataaaa gtaattctct tagcatatct aggcttgaaa aatatgctga atgtccattt
    12481 gcttatttta tacaatatgg tctaaaagca aaaaagcgta aagagtattc atttacacca
    12541 ccagatttag gaacttttat tcataatatt ttagataggt tttcaaaaga acttttacaa
    12601 gataatttaa cttggagaga tattgatgaa aaatatatag aattaaagat aggtataata
    12661 gttgatgaaa taattttaaa gataccagga tatatattaa atagttctga gaggtataaa
    12721 taccttgcat atagattaaa gaatatgttg acaactgcta taactataat tagtcagcaa
    12781 ataaaacaag gttcatttga accaatagat tatgaagtta aatttggcaa caatggaaaa
    12841 tatccaccta taaaaatggt gttagaaaat ggacaagaag taagtttaat tggacaaata
    12901 gatagagttg atgaatttga agaaggagaa aataaatata taagaataat agattataag
    12961 tcaggcaata agtccataag tctaacagaa atatattatg gtctacaatt acaattactt
    13021 gtgtatttag atgcaatcct tgaaagtgca aaggatgaag atataaatat aaatccagct
    13081 gcaatactat attgtagaat aaataatcca atagcaaaat ttaatgaaga taaagatgat
    13141 gaagaaatac aagaagcaat attaaaagaa cttagaatga aggggcttgt agtaaaagac
    13201 tctcacatag ttaaagagat ggataaatca ttaatagatg gagaaaggaa aaactcttta
    13261 gtaataccag taggtcttac taaggatgga aatgtaggta aaagtacttc agcaataagc
    13321 tatgaggatt tcaaactgct tagaaaatat gtaaggcatg ctataaagga tttatgtgaa
    13381 gaaatgttaa gtggagaaat acgtatagct ccatataaac ataaagatgg aacatcatgt
    13441 gacttttgtg attactctgc tatatgtcag tttgattcaa ctataaaaga taacaagtat
    13501 aaaaatttaa ataataagag taatgaagag ataataaaaa tgatgaaagg agatgttaat
    13561 tagtgagttc tcctaaatgg acaaaagagc aattagaagt tatagaaagt agagaatgca
    13621 atttattggt tgctgctgct gctggttcag gaaaaacagc cgttctagtt gagcgtataa
    13681 ttcaaatgat aacaagtaga gaaaatccaa ttgatataga taagttatta gttgttacat
    13741 ttactaatgc tgctgcgtca gaaatgagag agaggatagg ggatgctata ggaaaagcct
    13801 tagatgaaaa cccagaaaat aaacatttac aaaaccaact tgttttatta aataaatcta
    13861 gtattacaac aatacactca ttctgtttgg atgttataaa atcaaacttt catagaataa
    13921 acttagaccc taatttcagc ataggagacc aaactgaatg tgctattttg aaacaagaag
    13981 caatagagga agtatttgaa gatttatatg aagaaagaga tgaggggttt ttaaatttag
    14041 ttgaaagtta tgcagaaaga ggaggagata aagaagtaca agatattatc ttgggaatat
    14101 attcatttgc aatggcttct cctgaaccta aaaaatggtt gattgactca gcagaacgat
    14161 ttaatattga tgaaaatttt gatttttcac agtctatttg ggctagagct atattagata
    14221 ctgtaaaaat agagattaat ggtctttgtc taaatatgga aagggcatta aaagaagtgg
    14281 aatcaataga agaattagaa acttttgcag aaaagttatc cgttgaatat aaaaaaattg
    14341 ctgacatttc acaagcatgt aataaatctt gggatgaggc atataaaaag atggcaagta
    14401 tgtcctttga aaattatgta aaaggagtaa aaagaatttc aaaagatgct ccttcgtata
    14461 taaaagaatc aaaagaaaag gcaaaaacta taagagataa gactaagaag tctttagaaa
    14521 gtatagtaag tgctacattc aataaagata atgattctat tagagaagaa ataaaatatc
    14581 tttataacat agttaaacca atatctagtg ttgtactgag atttgaagag gaatatagca
    14641 ataagaaaag agaaaaagga ataatagatt ttaacgatat agagcatttt gcattaaata
    14701 tacttacaga tgtagatgaa aaaggtaata ttgtaccttc tgatatagca gttggatata
    14761 gaaacaagtt ttatgaaata tttatagatg aatatcagga tagtaatcta gtgcaggaag
    14821 tactgcttaa agctgttgca aatacagaaa ctccaaatag atttatggtt ggtgatgtaa
    14881 aacagagtat atatagattt agacaagcaa agccagaatt atttttgcaa aaatataata
    14941 actataatga taaaaaggga agctcccata gaaaaataat gctttacaaa aactttagaa
    15001 gtagagaaga agttgtagat gctgtaaatt atatatttga aaatataatg aatgagaata
    15061 taggagaaat tgaatataca gaaaaagaaa gactaaattt aggtgcaaat tttaatgtgg
    15121 atacagacga gaaatctatt attggtggag caacggagat acacctaata caaaaagata
    15181 ataaacttga tgatgatatt ataaatgata aagacgatag aattaataac aaagaaaatg
    15241 aaatagagga agaagaaaaa ttagataata tacaacttga ggctagaatg gttggaaaca
    15301 ttatcaaaga tttaatgaaa gtcaatgaag atggaaaaat tcaaaaggtc tatgataagg
    15361 gaatagatgg atatagacct gtggaattta gagatatagt tattctttta agagctacat
    15421 ctgcatgggc tccagtgttt gcagatgaat tgatgaatat ggatatacca acgtatgctg
    15481 atgttggagt ggggtatttt gatacgattg aaataaaaac aatattgtca ttacttcaaa
    15541 taatagataa tccaatgcaa gatatacctc ttatatctgt attaaaatca cctatatttg
    15601 gatttacacc agaggatttg attgatatta gagttcagag taaagataaa attttttatg
    15661 aggttttaaa aagtacagca gagtatgatg gatttacaga ttctcaaaat gaaaatgagt
    15721 ctgagtttat accaagtgaa gaatgcataa acaaaagtaa agatttttta attaagctaa
    15781 aggaatttaa agaaaagtca atgtacatga gtacagatga atttatatgg tacttgtaca
    15841 caaggactgg atactatgcc tatgttggag cattgccagg tggaagtcaa agacaagcta
    15901 atttaaaagt attgtttgag agagcaaaac aatttgaaga aaccagtctt aaaggaatat
    15961 tcaactttgt aaattttata gagaaattaa aaaaatctag ttctgatatg ggaagtgcaa
    16021 aaacacttgg tgagaatgca aatgttgtta gaataatgag tattcataaa agtaaaggtc
    16081 tagaatttcc agtagtcata tgctctgcaa tgggtaaaaa tttcaatact caagatttta
    16141 aaaagagtat tttatatcat cataatttag ggtatggacc tcaatttgta gactatgaga
    16201 gaaggatttc ttttccaagt atagccaaag aagcattaaa aagcaaaata aatatagaaa
    16261 atttatcaga agagatgaga gttttatatg ttgcatttac aagagctaaa gaaaaattga
    16321 ttataactgg ttctactaga aatatacaag atagtataaa gagatggtca aatggaattg
    16381 aaagtttaga tacaatatca caatacgaga tattaaaagg taaaaatttt ttagattgga
    16441 taatgccttg tgtattaaga catagggatt tatctaatct actagaagaa gtaggattag
    16501 acgcagtttt taatgttgaa cataattcaa aatggtatgg aaagttgtgg aataagagtg
    16561 atattttagt ggaaaagaag agtgatgaag aaaaagaaag tattgaagaa attcttgaaa
    16621 aaatagatgt agataatcct gatagtgatt attacagtga aatagaagaa aaattaaact
    16681 atatatatcc atatgaattt agtacaagaa aacctgctac tatatctgtt acagaaataa
    16741 aaaagataca aaataattat gaagaagagt taataaatac tatatttgaa caaaaagtaa
    16801 tattaaaaaa gcctctattt attcaaaatg aagaagaaag agaaaaaata agtggaacag
    16861 aaagaggtac aatagtccat ttagttatgg aggttttaga cttaaaaaat gtaagtagtg
    16921 taaatgatat aaaatcacaa ataagaggtt ttgtttcaaa aggtataata acagaaaaac
    16981 aagctagtat agtaaatcca tataaaatat ataaattttt tgcctctaac ataggaaaaa
    17041 gaatgttgaa tgctgaaatt ataaatagag aaaaatcaat ttatgcacaa gtaaatatga
    17101 aagacatata tatttatgaa aagcttataa acaatgacga taaaaaactt tatgataatg
    17161 aaagtgtcat gttaagaggt atagttgatg cttattttga agaagacaat caaatagttt
    17221 tggtggatta taaaacagat tttgtaaatg aggaaaatat aaatcaaata atagagaaat
    17281 ataaaaaaca gttggattta tatgcagata ttattgaaac tttaactggt aagtcagtaa
    17341 aagagaaatg tatatattta tttggagttg atgaagctgt ttgttattag tttttatata
    17401 taaattaaac ctaaaagata gaggaagggg aaaaaatgag atttattcat acatcagatt
    17461 ggcatctagg gaagtcccta gaaggccatt ctagaattga ggaacaagca aagttttgtg
    17521 aggaatttat aaaaatagta gaatcaaatg aaatagatat ggttataatt gctggagatg
    17581 tatatgatac ttctaatcca ccagcacaag cagaaaaact attttatcag actgtatcaa
    17641 gacttgccaa taatggtcag aggtgtgtat taataatttc aggaaatcat gataatccag
    17701 agagactctc tgcaataaca cctctagcac atgaacaggg gatacttata tatggttatc
    17761 cattaagtgc tacgatagaa gctaagtata aagggtttga aataacatat gcaactcagg
    17821 gatgtacaaa actgaatata aatggagaaa atattgtggt tgctacatta ccttatccaa
    17881 gtgaaaaaag attaaatgaa gttttttcaa gtgaagatga gtttgagaaa caaaaaaatt
    17941 attctgaaaa ggtaggagat atatttagaa gtttagaaga aaattttaga agtgatacta
    18001 taaatatagc cgtatcacat atatttgtaa taggtggaga gagtactgaa tcagaaagac
    18061 ctatacaatt aggtggaagt tttttagttg aaagaaaaga tttgccagaa aaggctcaat
    18121 atactgcact aggtcattta cataaacaac aaaaagcttc cgaacgttta aatgcatact
    18181 attctggttc tccacttcaa tatagcaagg atgaaagagc gtataccaaa ggggcataca
    18241 tagtggatat aaaagctggt gaaaaaccaa taattgaaga tgtatatttt aataattaca
    18301 aacctataga agtatttaaa tgtaatggaa tagaagaagc actagatata tgtgagcaaa
    18361 atcaagatag agagatttgg tcatattttg aaataaatac tgatgagata atatcacaga
    18421 atgaaattaa aaaaatgaag gaactattaa aagacataat agaaataaaa ccaataataa
    18481 catcttgtta tgaacaagaa agtgtagata taaaagaaaa gtctatggca gagttgttta
    18541 gagagttcta ttcttttagt aaaggtgtag agccaaaagg agaacttatg gatttatttc
    18601 tagatataat aagtgaagaa ggtgaatcag cagatgagac caattagatt agaacttaca
    18661 gggcttaata gctatattga taaacaagtt atagactttg aaaaattaat tgaaagaggt
    18721 ttgtttggga tatttggaac tactggaagt ggaaaatcta caatcttaga tgcaataact
    18781 attgctatgt atggaaatat atccagaaat acaaaagaat atataaacag tgtgtgcgat
    18841 aaggcaatta tatcatacga gtttgagata ggaagcaaaa acactagaag aagatacatt
    18901 gtagatagaa ccattgctag aagcaaaaca ggaacaaaga cctctaatgc tagattaata
    18961 gaagtattga atgacaatac ccaaaatgtt ttagctgata aagttgtgga agtaaatgaa
    19021 aaagtagcgc aggtcgtagg attaacggca aatgatttta ctaggtcagt agttttacca
    19081 caagacaaat ttaatgagtt tctaagatta tcacgtgcag atagaagaga catgttagag
    19141 agaattttta atcttgagaa atatggaaga agtcttggcg aaaaagtgaa aaagagaaaa
    19201 aatatacagt tacaaaattt aaaagatttg aaatcaaaat taagtcaata tgatggaatt
    19261 acagaggaag tatataacaa tataaaccaa gaattaatag agttaaaaaa tttggaaaaa
    19321 gataagaata atgctttaga cctagctcaa aaatcttatg aagaaagtaa aacagtatat
    19381 gaagaacaat taaagttaga aaaaaatgaa cttagaaaaa atgagcttga tttaaaaaat
    19441 agtgagataa aagaaaagat tgctcttgta gaaaatgata gtaacgctaa aaaggtagac
    19501 ccatacataa gttcagttca aaatctagaa aaaaagatag atgaagatag cttcgcagta
    19561 agtaatttag aaaagaaatt agcaatatta aatcaggagc ttgaagttac aaaaaataga
    19621 tatgaaaaaa taaacaaaat taagaatgaa gaaatcccta aattgagtga agaaaaaata
    19681 agattgcaac aagcaataaa attagaagaa gagttagttt tattagatag agagttaaaa
    19741 gagctaaaag aaagtggaat taatttaaat aagactaaag ttgaattaga aaaagtaaag
    19801 caggtgtcag agtccagaaa agatgcagtt actaaatcta taaaagaagt tgaaggaaag
    19861 attgataaag taaatataag tgcagaatta aaacaaaaaa tatttctagc atatgaatac
    19921 gagaaagatt acaacaacgt attggaagaa aaaaatcaaa agctaaataa actagaagaa
    19981 atactcaaag agacagaaaa tataaattta aaagttagat atattgataa agataagaat
    20041 gatgtaaatc gtaatttaga aaatttatca cttcacttgg atgttcttct taaaaaatgt
    20101 cctggtaaga gtgctgattt acttttaaaa tctgaatatg ttactgaatt aagaaataaa
    20161 gcaaataata ctaaagaaaa tgaaattaag aaaagcagta tacaagatga attaaagata
    20221 atacttgaaa gtaagtttaa tactgaaagg gaagtaaatt tactaaatga aaaacttgaa
    20281 aataatagaa agaacagaga tgacttagaa aaagaattag aagaactaaa gtatttaaat
    20341 ttagcctctg aacttagacg tgaattaaaa gaaaatatgc cttgtccagt ttgtgggtca
    20401 aaacatcatg aaaatcatga tataactaag tatgatgaaa atatatcttt tgtcaaagaa
    20461 aaactggaga aattagaaaa agaaaaaata agtataagaa acaatataga ggaattaaat
    20521 gctaaagtaa gtgggtattt atctatagaa aaaatgaaga ctaaagaact tgaagatgta
    20581 aaaggaaaac taggggggat tccatctagt caattattaa aaaaacttga tgaagaacaa
    20641 agaaaacttg cattgttaaa atctaatatt caggagtggg agaaagaaaa agagagtact
    20701 gaaaataaag taacattagc taaagaagaa aaaaataaaa ttgaaaaaga agaattaaaa
    20761 ataagagaga gtctgaataa ttataagaaa ttaacaagag atttaaacat agaaatagaa
    20821 ggtttagaaa ataaatgtaa aaaacttaaa caggaatacc taggattaaa gacgattact
    20881 aaagtctcta atctattgtc aaaagtaaaa gaaataaggg agaatgaaaa agaattagaa
    20941 ttactaagta ctgggcattc aaatttatta aaaaatagag actcactaga tattaaaata
    21001 agagaaaatg agagtaaact acatgaaata gagatagaat taataaaggc tagagaatta
    21061 tatgtagaaa agaaattgag tagagataat aagtataaag aagttattag cataacgaaa
    21121 ggtgatttag ctaaaaatct tttacataat gttgaagaaa atatatgtaa gatattagaa
    21181 caagaagaat caagtaagaa aaaattggaa gagcaaagat tagaatatga aaaaaacatt
    21241 gctgaaaagc acaatataga tggtagactt aaaactgcta aggaacaata taaagaccaa
    21301 aaatcaacac taaataaact attggcagaa aataaatttg aaagtatata tgcagtaaaa
    21361 agagctttat tagatactga tgcagtaaaa aaattgattg aagaaatact agagcatgaa
    21421 gaagaacaaa agttattatc atttaaaata aaatcatcaa aagaaaaatt aaatggaaga
    21481 agcataaaga aggagtactt tgaacagtta aaagatgaaa tttataattt gaaagtagaa
    21541 ataggaaata tatccaaaga tattggagct aatcaaaatc agttaataac attaaaagac
    21601 tctttagata agataaatga ttttaataaa caacttaagg tagttgaaca caatgtggac
    21661 ttattggaag aattagataa atgtgtacaa ggaaataaat ttgtagaata tgtttctaca
    21721 aaccaattaa aatatatagc gttggaagca tcaaaacgat tggatggaat aacaaaagga
    21781 agatatgcac ttgaaattga ctcaacacta aactttgtaa tgagagataa ctttaatgga
    21841 ggagaaagaa gaagcattga tactctatct ggtggagaga cattcttgac atctctatca
    21901 ttagcacttg ctctttcatc tcaaatacaa ttaaagggaa gtgcaccatt agaatttttc
    21961 tttttagatg aaggttttgg ttcattagat agtgaacttt tagaaattgt tatagaatcc
    22021 ttggaaagat tgcatagtaa taatctaagt gtgggtataa taagtcatgt cgaggaactt
    22081 aaaaatagag ttcctgttaa gctattagtt tcatctagtg aggctggaat aggttcaaaa
    22141 gtaaaaattg aatatagtta gaaaatatat gttaattgaa tatattatct aatatttcca
    22201 aataatatgt aattgtgata taataaaaac taatattaaa gttatttata attaatatta
    22261 atataaaaac atgtaagtag gagtgtggta agtgttggaa tcgcccaata atttgattca
    22321 gattattttt ttaatagtat tacttatagg gtctgcattt ttctcagcat ctgaaacggc
    22381 cttaatgtcg ttgagtaaaa tcagaatcag atatatgcaa gatgaaggag ttaaaggagc
    22441 taagttagta agttcattaa tagaaaatcc aaataagctt ttaagttcta tattggttgg
    22501 aaataacgtc gtaaatatag cagcaacatc catatcaaca tctttgttta taggattaat
    22561 gggagaaaaa ggagtggcac ttgctacagc tgttatgaca gtattagtat taatatttgg
    22621 tgagattact ccaaaaacaa tagcagcaaa taattcagaa aaggtttctc tattagtatc
    22681 taaacctata aaagcaatta tatttatatt aagaccaata gtatggatat ttaatataat
    22741 tacaaatatt atatttaaat tatttggtat aacaaataaa ggagctaaat cattcataac
    22801 tgaagaagag ctaaaaacta tggtaaatgt aagtcatgaa gaaggcgtcc ttgaaatgga
    22861 agagagagaa atcataaata atgtttttga atttggagat atgcaagcta aaaatgcaat
    22921 ggtacaaaga atagatatgg tagctataga tatggaagat agttatgatg agataataca
    22981 ggtatttaaa actgaaaaat taagtagaat gcctgtgtat gaagaaacta tagatgatat
    23041 tgtaggaata cttaatataa aagatattat atttttatct gatgaagaaa tagagtcatt
    23101 tgatattaaa aattatatga gagaaccttt tttcacgtat gagttcaaaa agataacaca
    23161 acttcttgaa gagatgaaac ttgaaaaaag tcagatggct atagttgtag atgagtatgg
    23221 tggaacatct ggacttttaa ctatagaaga tttagtagaa gtcattgttg gagatataga
    23281 ggatgaatat gatgaagaag aggatgaaat acaagttata aaagaagatg agtatattgt
    23341 agatggaagt actaaaattg gtgatgttaa tgaacttatt ggtgtaaact tggaatctga
    23401 ggagtttgat tccataggag gttttataat tggacaccta agcagattgc cagaagaaaa
    23461 tgaagtaatt gaagttgata atataagatt ttgtatagaa agtatagaaa aaaatagaat
    23521 taaaaaaata agaatatata cataatgtaa taatgttctt aaatttatta gaagtgtata
    23581 aatataaaag atatctttta tatttataca cttcttttta ttttgtattc aaataaaact
    23641 agta
//
LOCUS       DBGJFU010000055        23609 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.055, whole genome shotgun sequence.
ACCESSION   DBGJFU010000055 DBGJFU010000000
VERSION     DBGJFU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.055"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            474..1352
                     /locus_tag="ACOIAS_002032"
     CDS             474..1352
                     /locus_tag="ACOIAS_002032"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HGZ2701744.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIIAQKTNKNSKTISCIFQL
                     FDKDNCDEVDCLKTIKENMKNDKSEYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFA"
     gene            1501..4020
                     /locus_tag="ACOIAS_002033"
     CDS             1501..4020
                     /locus_tag="ACOIAS_002033"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2701745.1"
                     /translation="MTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCI
                     ILNQLEKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITEFKKIQE
                     ELDSKRFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLKDELNNKLI
                     KLIFKDDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLC
                     CVIVDITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDP
                     ITENATKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKAT
                     TLFDNNGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLD
                     KKRKGALMIIDIDDFKGINDNLGHLYGDAVLSEVANDLINLFRATDIVGRIGGDEFIV
                     FIKDISKTRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNA
                     DIALYSAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYE
                     SSDIEVAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGITSEINNLKNIC
                     YKDLGNYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGF
                     DECSYNRMWTNDEVNTLTFISKTLNMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVV
                     DRNTYELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFM
                     HNKKFEVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4205..6139
                     /locus_tag="ACOIAS_002034"
     CDS             4205..6139
                     /locus_tag="ACOIAS_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGZ2701746.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6245..7240
                     /locus_tag="ACOIAS_002035"
     CDS             6245..7240
                     /locus_tag="ACOIAS_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HGZ2701747.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7212..7997
                     /locus_tag="ACOIAS_002036"
     CDS             7212..7997
                     /locus_tag="ACOIAS_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701748.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7985..8683
                     /locus_tag="ACOIAS_002037"
     CDS             7985..8683
                     /locus_tag="ACOIAS_002037"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701749.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8806..9279
                     /gene="ybaK"
                     /locus_tag="ACOIAS_002038"
     CDS             8806..9279
                     /gene="ybaK"
                     /locus_tag="ACOIAS_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HGZ2701750.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9476..10315
                     /locus_tag="ACOIAS_002039"
     CDS             9476..10315
                     /locus_tag="ACOIAS_002039"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HGZ2701751.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10328..10876
                     /locus_tag="ACOIAS_002040"
     CDS             10328..10876
                     /locus_tag="ACOIAS_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HGZ2701752.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10942..12123
                     /locus_tag="ACOIAS_002041"
     CDS             10942..12123
                     /locus_tag="ACOIAS_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HGZ2701753.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPAPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12291..12677
                     /locus_tag="ACOIAS_002042"
     CDS             12291..12677
                     /locus_tag="ACOIAS_002042"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2701754.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDTDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12827..13090
                     /locus_tag="ACOIAS_002043"
     CDS             12827..13090
                     /locus_tag="ACOIAS_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2701755.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            13616..14299
                     /locus_tag="ACOIAS_002044"
     CDS             13616..14299
                     /locus_tag="ACOIAS_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2701756.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGKFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            14310..15551
                     /locus_tag="ACOIAS_002045"
     CDS             14310..15551
                     /locus_tag="ACOIAS_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2701757.1"
                     /translation="MKIFSNKDIKIFFLMLISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIILNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISVNGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSIFTISFLKLTNM"
     gene            15623..16306
                     /locus_tag="ACOIAS_002046"
     CDS             15623..16306
                     /locus_tag="ACOIAS_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701758.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            16303..18633
                     /locus_tag="ACOIAS_002047"
     CDS             16303..18633
                     /locus_tag="ACOIAS_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701759.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDNGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANIGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNGNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAVSGCLLGSILGLILHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPIKKICELDIIDSINAQ"
     gene            complement(18814..19656)
                     /locus_tag="ACOIAS_002048"
     CDS             complement(18814..19656)
                     /locus_tag="ACOIAS_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HGZ2701760.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19951..20685
                     /locus_tag="ACOIAS_002049"
     CDS             19951..20685
                     /locus_tag="ACOIAS_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2701761.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGRPIEYTISIFRTDKYQYEIVLSE"
     gene            20695..21828
                     /gene="nagA"
                     /locus_tag="ACOIAS_002050"
     CDS             20695..21828
                     /gene="nagA"
                     /locus_tag="ACOIAS_002050"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGZ2701762.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLNNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            21927..22676
                     /gene="nagB"
                     /locus_tag="ACOIAS_002051"
     CDS             21927..22676
                     /gene="nagB"
                     /locus_tag="ACOIAS_002051"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGZ2701763.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22828..23100
                     /locus_tag="ACOIAS_002052"
     CDS             22828..23100
                     /locus_tag="ACOIAS_002052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701764.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         9429 a         2467 c         4098 g         7615 t
ORIGIN      
        1 aaaggatatt ataatatttt actagaatta ttatctggta acttaagttt tgagtattaa
       61 tctaaagtat atgtttttat atggtttaaa ttttatgtat acaaaattaa tttgctaact
      121 gaatcatata aaaaaaaaca aaatattttt tagagggaat gattagatag aatttttttg
      181 ttatgaaatt aattgaagag taattaatta gaaaaggaat gaataataat gaatatatta
      241 cattattact ttttgataaa tcaaaaataa agttataatt tttaatatta attaagaatg
      301 atgaaatatg aataaagatt ttacaaataa atcttaagct atcttattta attctaatac
      361 aggaagaagg taaatgtata ttgaaaaaat catagaaata gaagtaaaca atttaaataa
      421 atacaaaggt ttgcaaaaga agtaatataa ttatatacaa tgaggatgtg agtatgatga
      481 acagactaaa gccaagaatt ttaggtatta gtatcttgtt tattattata attattttaa
      541 catgtggagc tgttttcaag tttaaattta aattagataa taaattaaaa gaaaccacac
      601 atacatattt gtctgagttt acagaacaca atataattaa tctcaagaca agacttagtg
      661 gtcaatttga catgcttgaa tctattgcta acttcatagg aaacttgaat aatataaatg
      721 atgaaatggt gattgattta atggattctg atgtaaagcg aagttcttta attagaatga
      781 ctatctctac attagatggg gaatgttatt ctaatgatgg aattacccat aatgttcaag
      841 atagagatta ttttaaaaaa gctaaaaatg gagaaaagaa tatatcagag cctttaaaat
      901 ctattgttaa taataaagaa ataatagttt tatctacccc agtttataaa agtgggaaaa
      961 tagttggtgt tttaagtgga atttatgatt caaaaaagtt aaatgaatta ttaggattat
     1021 ctgcatttga tgaaaaagca gatgtttttg ttgcaaagaa aaatggagat ataattgctc
     1081 aaaaaacaaa taaaaatagt aagacgatat catgtatatt tcagcttttt gacaaagata
     1141 attgtgatga agttgattgt ctaaaaacta taaaagaaaa tatgaaaaat gataagtctg
     1201 agtattttaa ttataactta gataatgaaa aatttatgtt aaactatcag ccacttggag
     1261 ttaatgattg gtatgttttt tctgttgtgc caaataaaat agtgtctatt cagtcagaga
     1321 atatatctta tgatgtatat atttttgcat gaaatactac tagttatatt aatttttata
     1381 atatatatag catgtattat aaataataac accaaaatta ttataaatga aaaagaaaaa
     1441 tttaaagtac tcactgataa tatactagga ggggttaaaa cctgtttaaa agatgaatat
     1501 atgactatga catatgtaag tcaggggttt ttagagctta caggttactc ttgtgaagat
     1561 ttaaataaaa tatttaaaaa tagatttaaa gacatgattt ataaagatga tttagacaag
     1621 tttaattgta ttattctaaa ccaattagaa aaacaagata aaattgaagt tgaatataga
     1681 attgtaagaa aagatggaag tataatttgg attttggaga gaagtaagtc tataaatgat
     1741 aataaaggaa gtgagtgtat tcagggagtt ttaactgata taacagaatt taagaaaata
     1801 caagaagagt tagattcaaa aagatttgaa atacaggcta tcaatgatag tattgttggt
     1861 ggaatagtga taactgagat aaatgatgat tttgatagta tatatttaaa tgaaggttat
     1921 ttaaatatga ttggatatac acgcaaacaa ctgaaagatg agctaaataa taaattaatc
     1981 aaacttattt ttaaagatga tagaacagaa ttaattaata gtataaacaa acaactcaaa
     2041 ttaagtgata catttgcatc tgaacaaaga gttgaaaaaa gggatggcac tattatatgg
     2101 attttattaa aaggaagaaa gatgatttat gataatggaa aatctgtact ttgttgtgtt
     2161 attgtagata ttacttctgc taaattatta gagcaagaac ttaaaaagag tgaagaacgt
     2221 catgaaatca ttctaaagca gacgcaaaat attataatag aatgggatat agaaaaagat
     2281 aaaatgaata tatcacctaa ttggtataat aagtttaaat atgaccctat aactgaaaac
     2341 gcaacaaaaa atataattaa atcagtagat atatatcatg atgatatgga taagtttatt
     2401 tcattaatta aacttacttc acaaggtagt gagtatgaag agtgtgaaat tagacttaga
     2461 actagagata ctatttacgc ttggtttagt ataaaggcaa caactttatt tgataataat
     2521 ggtaaacctt ataaagtagt aggaagtttg actgatattg atgaatcaaa acgagaagct
     2581 gaaaagttga aagaaagggc agaaaaagac cctcttacag atttgtataa taaaaaagca
     2641 tcacaatcac ttatagaaga gtacataaca ttggataaaa aaagaaaagg tgctttaatg
     2701 attattgata ttgatgactt taaaggaata aatgataatt taggacattt atatggggat
     2761 gctgtattaa gtgaagttgc aaatgaccta ataaatctat ttagagcaac cgatatagta
     2821 ggcagaattg gtggagatga gtttattgta tttataaaag atatttctaa aacaagagat
     2881 attataaata aagcagaaga attggtaaaa acatttgaaa ggtctttttt aggtaaagga
     2941 cgaaattata aaatatcatt aagtgtagga attgctaagt ttccacaaga tggtatggac
     3001 tatatgacac tatttagaaa tgcggatata gctctttact cagccaaagg gaagggcaag
     3061 aattgttata cattgtatga taaaaaaatt gataaagtcc aatatataac taatacaaga
     3121 aatgcaggag atagtgataa tgtagagatg tcttttaaag aacatatcac aaattatata
     3181 tttgatatac tatatgaatc aagtgatata gaagtggcag taaatttaat tctaagtatt
     3241 gtaggtaaac actttaatgt gagtcgcatg tatatatttg aaaattcaga tgataatcga
     3301 gtatgtaaaa atacatttga atggtgtaac gaaggtataa cctcggaaat taataatctt
     3361 aaaaatattt gttacaagga tttaggcaac tatcttttaa attttaatgc agatgggtta
     3421 ttttattgta atgatattga aaatttaggt agtgatgtga aaaaagtact taagccacaa
     3481 ggcatacgtt caatattaca atgtaaaatt ttagagggta atgaatttag agggttcata
     3541 ggatttgatg aatgttcata taatcgtatg tggactaatg atgaagtgaa cactttgaca
     3601 tttatatcta aaacgctcaa tatgttttta ttaaaaatgc gtattcagag taagctagaa
     3661 gagtctttaa caattcaaaa atctgtgatg gataatatgg atatatgggc atatgttgtt
     3721 gacaggaata cctatgaatt actatttatc aataaaaaaa tgcttgagtc agcaccaaac
     3781 actcatgtag gtgatttatg ttataaagcg atatggaata atcaagataa accttgtaaa
     3841 aaatgcccta tgatatattt aaaagatggt gatgatactt ataaatcatt catgcataat
     3901 aaaaagtttg aggtacaggc aaatataact acaactactt tgaaatggtt tgatggtaaa
     3961 gatgcatgtt taatatttgg aagtgaaata actagtattg aagctgaaga aaatacttaa
     4021 aatactgtgt tatatatata cttaaaatcg caatttgaat ttattagact taaaatgtct
     4081 atataaatca tttaagttgt ggttttaagt attttttatg atataattaa gcgttaagaa
     4141 caaaattaaa agatactttt gtataaaaaa atatttgtta aatatatatt gagcaggtga
     4201 taatatggaa aataagtttt taccaataag caaacaagat atgatagata gaggatggga
     4261 agaattagat tttgttctag ttacaggaga tgcttatgtg gaccatcata gttttggaac
     4321 agcaataata tctagagttt tagaaagtgc tgggtataaa gttggaataa tagctcaacc
     4381 agactggaga actacagatg attttatgaa gctaggaaaa cctagacttg catttctagt
     4441 taatgcagga aatatggatt caatggtcaa tcattattct gtaagtaaaa agcatagaga
     4501 aaaggatatg tattctccag gtgggaaaat gggttataga ccagatagag ctactatagt
     4561 atattgcaat aaaataagag aagcatacag tgatgttgca atagtaatag gtggaattga
     4621 agcgagctta agaagatttg cacactatga ttattggagt gataaggtta gaaagtctat
     4681 gttaatagat agtggagcag atttattagt ttatggtatg agtgaaaagc agattgtaga
     4741 agtagccaat gcacttaatg atggatatga cccaaaatac attagacata ttgatgggac
     4801 atgctatatt tcagatacat tggaagaaat atatgataaa tatatattaa ttccatcata
     4861 taaagaaatt tgtgaagata aaatgaagta tgtagaagct tttaaaattc aatatgatga
     4921 gcaagaccca tttagaggta atataattgt tcaacctcat ggaagtaagt atctggttca
     4981 aaataagcca gaaaaaccac ttagtagaga agaactagat gaagtatatg gattaccata
     5041 tcagaagaca taccatcctg tgtatgaaaa atttggagga ataccagcta tagaagaagt
     5101 aaaatttagt atagtaagtt caagaggctg ttttggtagt tgttcatttt gtgcaataac
     5161 atttcatcaa ggtagagcag tgcaaagtag aagtgaaaaa tcaattattg atgaagcaat
     5221 aggaataaca aatttagatg attttaaagg gtatatacat gatgttggag gtccaacagc
     5281 taattttaga agaccagcat gtaaaaaaca aattactaaa ggtgcttgta aaaatagaca
     5341 atgcttatct ccatcaccat gtaaaaattt agatgcagac cacagtgaat acttacattt
     5401 acttagagct gtaagaaaat tacctaaaat aaaaaaggtg tttgtgcgtt ctggaattag
     5461 atacgattat gtaatggcag ataagaacaa taaatttctt agagagttga tagagcatca
     5521 tgtaagtggt cagttaaagg tagccccaga acatatatca gaagaagttt taaagtatat
     5581 gcaaaaacca gcaggaaaaa cttatgataa atttagacag aaattttttg ctataaatga
     5641 agaattaggt aaaaagcagt atttgatacc ctatttaatg tctagtcacc ctggttctac
     5701 cttaaatagc gctatagagt tagcagagta tttaagagat actcactacc aaccagagca
     5761 ggttcaggat ttttatccaa ctccagggac tctatctaca accatgtttt atacaggtat
     5821 tgacccgctt acaatgaagc cagtttatgt tcctaaatca aaacgtgaca aggcaatgca
     5881 aagggcttta ttacaatata gagcacctag aaattatgat ttggtatact ctgcgttagt
     5941 tgaagcagga agagaagact taattggttt tggacataga tgtcttataa agccaaaaaa
     6001 tgaaaagcct tattttaata gaaataattc taaaaaaaat gtatctaaag gaacaaataa
     6061 aaacaaaaaa actaatacaa ataacagaag taataaaaat caacaaaaaa gttcaactaa
     6121 aaaaaagaaa aaacattaaa gtataaataa aaatacttaa aaaagaagga ttattcgata
     6181 aattaacaga aattagtata aataaatata attattttaa tgacgttacc tttgggagga
     6241 agagatgaaa aagtatattt ctataatatt attagtggtg ttgactatgg ttgttttggt
     6301 aggatgttca ccaggaaaag ataaccctaa aaataaggag ctatcagttg tcaaacaagt
     6361 aaaagtagct gttccagatg gactaccagc agtggcaata gctaaacttg caaatgaaaa
     6421 tccagagatt aaagaaggat atgaaacagt gtatagcatt gaaaagacac ctgaagcgat
     6481 ttccactaga gtgatgaaaa aagatgcaga tatagctata gttccttcaa acatggctgc
     6541 aatagcgtat aataaaactt catcatataa tattgttgga actgtaggaa tgggttcact
     6601 ttatttggtg tcaacagaaa atataaaaga ttactctgat ttagttggta aagaagttgg
     6661 atgtactgga aaaggactta ctccagacat aactataaaa agtcttttgt tacaaaagga
     6721 tataaattat tcagatataa aatttaatta tgtaaattca gcaagtgaac tggtaccatt
     6781 attggctact ggtaaagtta aaacaggtat tgttccagaa cctgcactta gtgctttgat
     6841 gtctaaaaat ccagacataa aaattataaa aagtttaaat gactcatgga aggaagtaag
     6901 tggttcaaaa gatggttatc ctcaatctac tttaattgta aagtctgaat ttttaagaga
     6961 taataaagat tttgttgatt cttttgtagg tcaattatca aatagtatag attgggcaaa
     7021 caaaaatcct gaagaattag gtgcatattc tgaaaaaata aaaatatcta ctgaatcaaa
     7081 gataatagga aaatctcttg aaagagctaa tttgaaatat attccagtta aggacatgat
     7141 taaagattat aaaaattact atgagaaatt agctaatttt gatgataaga cattaggagg
     7201 caaagtacca gatgaagcaa tatactttgt tgagaaataa aaaaaataaa gaacaattta
     7261 tagataaaat ggaggtaaca ctttcttgtg ttatcctctt atttttatgg caaattattg
     7321 ctttaaaaat tgataatgat atttttttac caacagtagg tcaagtgttt aatagtataa
     7381 aagagattat gctaaattct agtttctata tagatatact ttactctatg gggagatgtt
     7441 tttttagttt tttactggct atgatttttg ctattatact ttcaatagtg tcttatttaa
     7501 atagattttt tagaaactta ttaaagccaa tcaatgcttt gacacgttca ataccaacta
     7561 tgatattggt agtacttgca cttatttggt ttgaaaagga tagtacccct tttatagttg
     7621 gatttactat agtttttcct atattgtatg ataacgtttt gggcgctata ttgaatatag
     7681 acaagaactt attagaaatg gctaatgtgt ataaaataag gtttatagat aaggtgttaa
     7741 aaatttattt accagccata aaatttcaaa ttatatctat actagtatca acattctctt
     7801 taagtttgaa agttgctata gcaggagagg tatatagtca acctacatat ggtattggaa
     7861 ctatgataca gactgaaaaa attaacttta acacatctgg aatatttgca tggataatta
     7921 ttgttctttt aatatctgct attttacaaa tagctcaaaa gtttttagca aggagagctt
     7981 ttttatggaa aagataaagt tgagtattga aaatataaat aaaagatatg atagtcgtat
     8041 tatatttaga gattttaata ttgattttta tgtaaatgag gtaaattgta tccttggaaa
     8101 atctggttgt ggaaaaacta cactcttgaa cataataagt gggattatta aaaatgacac
     8161 caacaatcta aatatcaaag aaaatttaaa tagagtagga aataagcttg aagctagtta
     8221 catatttcaa gatgatagac ttatagactg gttaacagta gaagaaaata taaagatagt
     8281 tgtaaataaa tactataata aaactcaatt aaataaaatt tgtgatgaat acttggaatt
     8341 ggttggaatt agtgattata aaaaattcta tcctcaaatg cttagtggag gtattagaca
     8401 aagagtaaat attgctagag cttttatata tccatcaaaa aacattataa tggatgaacc
     8461 ttttaaatct attgatgcaa agaatacaca gcttataatg gataatttca gaaatatatt
     8521 aagaaaagaa aagagaactg tgttatttgt aactcataat attgaggaag cattgttttt
     8581 agcagatagg atatttattt taggagactc tccaattaga ataaaaaaaa tattaaaaaa
     8641 ttctaaggaa ttggaaaaaa atgaagtttt gaagttgata tagagttata ataatactgt
     8701 cgttttaaaa tatatttggt taaaatctat aagactatat taaaatagta cagaatattc
     8761 aaaactgtgt ataatgttcg tattttataa aaaggaagtg aatgtatggt taagactaat
     8821 gctatgagaa tattagattc aaataagatt gattataaag taatgtctta tgaagtgaaa
     8881 agtgaacatg tggatggtgt tgaggtggca catgatatag gaagagatgt aaatgaagtg
     8941 tataagacat tagtaactca aggagttagt aagaatatat atgtatatgt aataccagta
     9001 catgaaaact tagacttaaa aaaggctgca aaagtagcta aagagaaaag tgtagagatg
     9061 attcatgtaa aagatataaa taagcttaca ggttatatta gaggcggatg ttctcctata
     9121 ggtatgaaaa agctttataa aacttttgta aatgaaagtg caaagaattt agatacaatt
     9181 attgtgagtg caggaaaaat aggatatcaa atagaactat ctccatttga tttacaaaga
     9241 cttataaaag tagaatttgt agatgtaata aaaaaataag aattacatat aaaatataga
     9301 gcttatattc tcaataatag gtgttgtaat attaataaaa tgaataataa ttttaaaata
     9361 aatattcagt tacttatgtg taattggtat aaaaatattg agaaatttta aaaacaatgg
     9421 taaagtgtaa gtataatcac gaatattttt tataaagtaa tggagggatg ttcgcttgag
     9481 aaaaataaaa tctgaacaaa tcgtcgagca ggtaaaaaaa ctttgtatag aagcgagcct
     9541 atatttagga gaagatgttt taagttgtat aaaagaaaaa gcgaaaagcg aaaagagtga
     9601 agttggaaaa aatatattga atatattagt tgaaaatgct gaaatagcta aagaaaaaaa
     9661 tataccaata tgccaagata ctggtatggc tgtatttttt gttgaaatag gacaagaagt
     9721 tttaattgaa ggagacacac ttactgatgc tataaatgag ggagtaagac aagggtatga
     9781 agaaggctac ttaagaaaat ctgttgtaag tccaattaac agagtaaata caaaagataa
     9841 tacacctgct gttattcatt atgatatggt aaaaggtgat aaaattaaaa tagaatttgc
     9901 agctaaggga tttggaagcg aaaatatgag taagatgaaa atgttaaagc catcagatgg
     9961 attggaagga attaaaaaat tcataataga tacagtttca gaagctggac ctaacccatg
    10021 ccctcctatg gtaattggag ttggtatagg tgggactgtg gacaaatgtg ctcaaatagc
    10081 taaaaaggca ctttttagag aactaggtga atttaataag gacgaaaata tagcaaaact
    10141 tgaaagtgaa ctattaacag ctattaataa acttggtata ggacctcaag gcttaggtgg
    10201 tactacaaca gctttgggat taaacataga gacttttcct acacacatag caggtctacc
    10261 tgttgttgta aatataaact gccatgcatc aagacataag aaggtagtaa tttaggagga
    10321 aaataaaatg ataaaaataa ctacacctgt aaatgaaatt gatatagcaa aattaaattg
    10381 tggagataca attagtttga gtggtatact ttatacagca agagatgctg ctcataaaag
    10441 attgatagat tgtataaata aaggtgaaga attaccattt gacgtatatg gtcaaggaat
    10501 atattatgta ggacctactc ctacaaagcc aggagaagtt ataggtgctg caggtccaac
    10561 gacgagttat agaatggatg atttgactat acctctttta gaaagaggtc ttagattgat
    10621 gataggcaag ggaaaaagaa gtgatgaagt tatagaaggt atgcaaaaat acggagcagt
    10681 ttatcttgct gcaataggag gagctggagc gtatatttca aattcaataa aatcatgtga
    10741 aattattgca tatgaagatt taggagctga agctataaga aagattgtag tagaagacct
    10801 taagcttaca gtagcaatag atagctatgg gaataatata tatgagcaag gtcgagcaat
    10861 ttatgaatgt aaataatgtt tagaaataaa tatgaatatc aaaattaaaa atagttgtgt
    10921 atattttaag gaggaaaatt gatgtctaag gattatgcaa aattggcatt agaaatgcat
    10981 gaaatcaaca aagggaaagt aagtgtagaa agcaaggtag aagttaaaac taaggatgat
    11041 ttatcaacag catatacacc aggggttgca gaaccttgtt taaaaataca tgaaaatcaa
    11101 gatgatgttt atagatacac ttctaaaggg aatctagtag cagtagttag tgatggaagt
    11161 gctgtacttg gattaggtaa cattggtgct gaagcatcta tacctgttat ggaaggtaaa
    11221 gctatattgt ttaaacaatt tgcagatgta gacgcttttc caatatgttt aaagactaat
    11281 gatgtagatg agatagttaa atctgtagag ttgatggaac ctgtatttgg aggtataaat
    11341 ttggaagaca tatcttctcc aagatgtttt gagatagaag agagattaaa aaaatcttta
    11401 agtatacctg tttttcatga tgaccaacat gggacagcaa taatagttgc agcagccata
    11461 ataaactcaa taaagttaat tgaaaataaa aaaatagaag atttagaaat agttataaac
    11521 ggagcaggag ctgctggtat agccatagca aagatactac ttaatatgaa tgttaaaaat
    11581 ataatcttat gtgatagaag tggagcattg gaagctagta tagaaaatct aaactatgtt
    11641 caaaaagaaa tgttaaaagt aacaaatata agaaatgaaa aaggaccttt aaaagatatt
    11701 ataaaaggaa aagatgtatt tattggtgtt tcaggtccag gagctgttac aaaggaaatg
    11761 gttgaaagta tgtctgagaa gccaataata ttggcaatgg caaatcctgc tccagagata
    11821 atgccagaag aagcaaaatt aggtggagca tttataatgg gaacaggtcg ttcagacttc
    11881 cctaatcaag tcaataatgt attggctttt cctggaatat ttagaggagc attagatgtt
    11941 aaggctaaag agataaatga agaaatgaag atagctgctg catatgcaat tgctaataca
    12001 ataagtgatg aggaaatatg cccagagtat atattaccag atgtatttaa taagaatgta
    12061 gtaaaaaatg ttgctaaggc agttaaagaa gcagctataa aaactaaagt aaatagaata
    12121 taaattttta tatgctcata tattcttact tttatataca tatctttatg attaaacgtg
    12181 ttagaaaacg tagtaaattt atactatcag actttacctt tattacttaa aatgatataa
    12241 tatgaatatt ataaattaat aaatttaaat ttattgaggt gcaagagata atggaatata
    12301 aagttaatga ccaagaactt aatgcttcaa tgtttatccc attcgttaac aggatatggc
    12361 caggagatta tgatacggat aaaacacaga ctgcattatc aaaaactttg aatataactg
    12421 cttatgatga taaagtgctt gtaggatgtc tgcgagttct ttcagatggt tattattttg
    12481 gaacaattac agaattgctt gttcttccag agtatcagaa acaaggcatt ggtagtaagc
    12541 ttttccagct tgctaaagag aatacaccaa ctatgctata ttttggtgca cagccaggag
    12601 tagaagagtt ctatgaaaaa aatggatgcc aaagaagtct tcaatcttac ataataaaaa
    12661 agaaagatag ttattaaagt tccagctttc aataatctta aaagtacaat attttagtgg
    12721 aatcatttca ttttttatta ctaatatgac ataaacatgt catattagta ttgatatact
    12781 tttaatatgg ttaagagcca aagaaaaata taaggagtga tttaaaatgg aagaaactaa
    12841 attttgccaa tgctgtgcaa tgccaatggg acaaactgat gaattatatg ggacaaataa
    12901 agatggaagt aaaagtactg attattgtag ttattgtttt aaagatggtg agtttacagc
    12961 tgatataagt atggaagaga tgatagaagt ttgtatccct catatgcttc aaagtaataa
    13021 ggatatgact gaagatgaag caagaacaat gatgaacaat ttctttccaa cattaaaacg
    13081 ctggaaataa ttttatgcaa aattagttat atgcaaaaca tgaaatatgg tgttgagaat
    13141 aaggggtggt gtgctttata gcataccacc tgttttgtta cttgctagtt caggtattct
    13201 tgtatgctta aatatgttct catctctctt tctattttaa attgtaaaaa tctaaaatat
    13261 gttatttata ttctacttct ttatccatgt ctttttctac cacaagtcaa atccaactac
    13321 aaaatcatca ttttcaattc actgtttaat agaattaaca gataaagagt tataaaattt
    13381 tctatttttt atataaaata atacaggtca agctgacttc tagctacttg ggatagtgta
    13441 tatatttatg atagttatag gagtaatacc aatcattaga ctgttgattt gtgtagtctt
    13501 ttgtaaaaac atttgtttta atatattgag tttttgaaaa agtaaaatta taattataat
    13561 aaattaagtg taataatgta ttagaagtaa aaataattaa aatagaggtg attaaatgag
    13621 tagtatattg ttagttgaag atgatttaag cttgatatat ggattagagt tctctataca
    13681 aaaaagtgga tttagtatag acatagcaag gacagtaaaa gaagctatgc aaatgtatga
    13741 ggaaaaaaat tatgacttat tgcttttaga tgtatcccta cctgatggta atggatttca
    13801 gatttgcgaa aaagtacgag aggtttctaa tatacccatt atatttttga ctgcttcaga
    13861 tgaagaggta aatgtagtga tgggtcttga tatgggtgga gacgattata taacaaaacc
    13921 ttttaaatta aatgaattaa tttcaagaat aaaagcatta cttagacgtt ataatgttgc
    13981 aagtaatgtg aatgaattaa gttcaaataa tattactatt aaattacttg aaaatcgtgt
    14041 ttttaaaggt aaatttgagg tggaacttac cacagcagaa tataaattat tgtgcttatt
    14101 tatgaaaaat aaaaatattg tacttactag aaaaaacata ttggacaagt tatgggatgg
    14161 aaatggtagc tttatagatg acaatacatt atcagtatat gtaagacgat tgagaaataa
    14221 aattgaagat aatcctgaaa atcctaagtt tctattgacc gttagaagaa tgggttataa
    14281 gtggaatgta attaagtgag ggtattaata tgaagatatt ttctaataaa gatataaaaa
    14341 tatttttttt aatgcttata tctattttag taatgtttat tatagtaggg caaatagtgg
    14401 ttataagtat aactaacaat tataaaaaaa cgttgataga gcatgattat aatatagctg
    14461 gatacttata tagtataggt gttgataaat ctaaaatacc atcagcattt gttgcacatg
    14521 aagataaata tattttaaaa ggaaaagaaa tactagatac agctggatat ggatttgata
    14581 tagatgttag ctttacacca agtgtaaaat tgtttcaaag taaatatata aaaactatgt
    14641 ctatctattt attagtattt tttactatga ttatttttat actatatatg tattttttta
    14701 ggcaacaaag taagatagaa gaggctagtt ataaaattga tttatttatg aatggaaata
    14761 taaatgaaag actaaataca tatgaagaag gtagcttatc aaaattattt agttcaatag
    14821 atgttatgtc gacatcttta aatacacata tattaaaaga aaaacagagc agagaatttc
    14881 ttaaaaatac aatttcggat atatcgcatc aattaaaaac tccacttaca gctttaataa
    14941 tgtacaatca gatactacaa gaagaaagtc ataatagtga tgtcgttgaa gcttttgtac
    15001 aaaaaagtca aagtgaacta gaacgcattg aaacattgat taagaattta ttaaagatta
    15061 caaagataga ttcaaaaaca ataattctca ataaaaataa cgttaatctt aaaaatttaa
    15121 ttgtcaaaat tttgtatagc tttgaaacta gggcagaaaa agaaggaaag gttatttctg
    15181 taaatggttc agaaaataca ttgttgttct gcgattatga atggatgtca gaggctttaa
    15241 gtaatcttat aaagaatgca ttagataata caaaagagaa tgataaaata acgatagagt
    15301 ggtttgagac gactataacc accattgttt cagttaaaga tactggaaat ggaatccatg
    15361 ttgaggatat tcatcatata tttaaaaggt tttatagaag taagttttca aaagataatc
    15421 aaggtttagg actgggatta ccactagtta aatctattgt ggaacaacat aatggtacta
    15481 tttctgtaga aagtaaacta tcacaaggaa gtatattcac cataagtttt ttaaagctta
    15541 ctaatatgta agataaaata catcttgcag taaggtatat agattattat tatattaatc
    15601 aattagaaag gtgagagaga aaatggatat tcttgtggtt aataatttat gtaaaacata
    15661 tggaaatgga tatacaaagg tagaggcatt gaaagatgta tcctttacag tttcaaaagg
    15721 agagtttgtg actgtagttg gagaatctgg ttcaggaaag agtacactat taaatctctt
    15781 aggaggtctt gatactccaa catcagggag aatatttatt gatggtaaag aaatatgttc
    15841 aatgaaagag gaaaaacaga ctatttttag acgtcgtaac atagggttta tatttcaggc
    15901 atataacctt ataccagaac taactgtgga gcaaaatata atattcccat tgttattaga
    15961 ttataaaaaa ccagatacta tttacttaga tgaagtattg actattttag gtcttaaaga
    16021 taggaaaaat catcttccaa atcaattatc tggtggtcaa caacagagag ttgcaattgg
    16081 aagggctttg attacaaagc caagtattat actggcagat gagccaacgg gaaatcttga
    16141 cagtaaaaat agtaatgatg taatttcact tttaaaagtg actgcagaaa agtataatca
    16201 aaccattatt atgataactc ataatacgaa tatagcttct acagcagata gaatactaca
    16261 ggtttctgat ggtgtgctta cagacctggg ggtgaataga gaatgagaag ctacttagaa
    16321 ttagtaccac aatatgcaaa agtacataag aaaaaaaata gaactacaat tttatgtatt
    16381 attattgcag tatgcttagt tactgctatc tttggacttg cagatatggc tatacaatgt
    16441 caaaagatac aatttattaa agatgatgga aattggcaca tagtaattag aaatattgat
    16501 gaaaagttta tatcagaaat aaatagtaga gtagatatag ctcaccatgg tagggggcaa
    16561 gtcgttgaca atggagagtt taaatcaaag gagattttga tacaaggttt agatgaagaa
    16621 gtagcaaagc aatttaactt agaaataata aggggtcgtt atcctattgg ggagaatgag
    16681 actttaatag atgaggatgc agttaaaagc tttaatctaa aaataggaga ttttatcaaa
    16741 ataaaattag cagatggtag aaataaaaat tataagatag taggtgaata ttcatcaata
    16801 tcaagcttaa gagctacaga taaacatgga ttaatacttt caatattggg tacacaagct
    16861 attaaaaaat cagaaacgta cgattattat tatattacat ttaaagaaaa ggttaatatt
    16921 agaaaagcca cttctgatat taaaaatacc ttcaatttaa gtgataaaca aattggtaaa
    16981 aatgaaagat tactttctat tataggtcaa ggtgactctg acttttctaa accattgtat
    17041 ttaacagcag gattactttt tgtacttgtt ttagtagcaa gtattatcat gatatataat
    17101 agttttaaca tgagtgtaat tgagagagtt aaattttttg gtctgttacg ttgtctaggg
    17161 tcatcaaaac atcaagttaa aaaatttgtt gttttagaaa gtttaatttt aagtataaaa
    17221 gctattccaa taggcttagt attaggaagt cttgtaacta ttatatcttc tatatttctt
    17281 aagtatgtta atgagttatt cagctcaatg ccaattttaa aagttagttt tattggtatt
    17341 gcatttggaa taattgtagg gtttttaact gtaatattat cagctattat tccagcaaaa
    17401 aaagcttccc gtgtatctcc acttagcgct gtacgtggga atctaatgtc caaagaaatt
    17461 ttaaataacg ggaagacaag taaagttgca aatataggaa gttttaaaat tgagacttct
    17521 atgggattaa atcatgcttt ttcaagaaag aaatcttttg tacttatgac ttcttcattt
    17581 gccattagta ttatactatt tttaggtttt agtgtgctta tagaccttat gtatcaagga
    17641 cttaagccta tgaagccaga agctcctcat atatctattg ttgaccctaa ttttaagtct
    17701 gttttaacta aggaagatat taatgaaata agcaatatca aaggagtaaa gaaagtatac
    17761 agtagaatga tgacggatat agaagctaca tataatggga agaagacaaa atcacaatta
    17821 atttcttatg aaaagtatca atttaattgg gcaaaaaaat atctattaaa aggagaagtt
    17881 aatgaaaata aactacagtc agaaaacctt gcattagtag aagaggggca tggatttgat
    17941 ataggagata agatatatat taaaaatgga aatgctgaaa aagagttaga ggtaggaggt
    18001 atactttcct caattccatt tgaggctaaa gataaatata ttggaaatat tataacatct
    18061 gaaaatactt ttacgaatat aacaggtata aagggttata taataatgga tattcaggtt
    18121 gataaagata ctcctggaga tttaattgaa aatataagac gtgtagtaga tgataactta
    18181 gaggtcagag ataaaagaca aagtaactta gaggttaaaa attcgtttta tactatggca
    18241 acatttgtat atggatttgt gtttattatt gcgattataa gcatgtttaa tatcataaat
    18301 agcatgaata taagtgttac aagtagaaag aattactatg gtgttatgcg tgcagttggc
    18361 atatccaaca gacaattaag aagaatggta atagtagagg caggttcata tgcagtgagt
    18421 ggttgtttat taggtagtat actaggatta atattacata ggtatatgtt tatatcgtta
    18481 gtaagctcaa tgtttcattt agagtggcaa gtacctttta atttactact tatcactgta
    18541 ctaactatga taataataac tttattatca gtaagaaaac caatcaaaaa gatttgtgaa
    18601 ttagatataa tagactcaat aaatgcacaa taaatatagg tattgattta aattaaaaaa
    18661 acgaagtaaa aatgaaatag aaaatatttt tcatttttac ttcgtttttt atattataaa
    18721 atttttgtta tgttacaaaa tttctataaa gatgatgtga atctaaacat tatatgacaa
    18781 cgtgagcatt taagttaaaa tattttaaat atactatttt agataagttc cctcttcatc
    18841 ttgataaatt aatccttttt tcataagcac tccaagagct cgtttaaaat ttttcttact
    18901 tgaattaaat aatatagaaa tatctttagg gtcagattta tcattaaatc tcatatatcc
    18961 atcagaacct tctaaataac ttaaaatctt attttctaaa gtatcaagtt catcctttct
    19021 atttcctcta ggagaaagac caagttttcc atcttcatat atttttatga catgtaaatt
    19081 ttcaagaaca tcaccagttt ttagttcagt aaagtactca ttatgaagta ttacaccagc
    19141 atatttattg tctacacata tcattgcaga attattagtt tggaatccat atacaactcc
    19201 agttacaata tctcctacat tgtaagtatg gtctgttaat aagtatgaat ctatatctgt
    19261 agtagcagca attcttttgc ttttgtctaa atatatataa aataaatatt tttcgtctct
    19321 aaatagtgga taagtcttag ctttaaaagg aactaatatg tctttaggaa gtcccatatc
    19381 tataaaagta ccaatgtctg tattatcaac tacttttaaa tgtgctacat ccccaacctt
    19441 agctaaaggc ggtattaaag ttgcagcagt tctgtcatca gaatctttaa atatgaaagc
    19501 atttacttca tcacctatgt ttatttcatt ttttccgatt aatctattat gaagaagtat
    19561 atcgtctttt gtgttattag tttgtccatc caaaaaatat ccaaattcag tttttctttt
    19621 tactgtaagt ttattaaaat cacctatgtt tatcaaatta aaatcatcct ctcattttat
    19681 atttataatt tagccataat atacttatgt agtagtttgt cacagtatct atattagtat
    19741 tagtgaaatt tacaataaat aatatagtta tttatgtatt tttcaattct ctagcttctt
    19801 atatagtata aactccaatt tcttttttgt ctttaggttt tattaaaatt tttacaaaat
    19861 aagttaaaaa gtattgaaat aaaatttctt ttaaagtata attggtatat accaaaattg
    19921 gtatatacca tatcgaaaag tggtgactat atgaaaatag taaataaatc tagtaatata
    19981 ccattgcata ctcaattaag ttcaataata agagagatga tagaaactgg tgagcttaaa
    20041 gagggcgatg caataatgcc agaaagagaa ctttgcaaca tacaaaatgt aagtcgtatg
    20101 acagtaaata aaaccattgt tggattagtt acagaaggac ttctttatag ggtacaaggt
    20161 aaagggacat tcgttgcaaa gcaaaaaaag aagtatcagt tttcaaatgt aaaaggtttt
    20221 actgatgtta tgaaagaaaa gggagtaaac ataaaaacgg atatattatc ctttgagatg
    20281 gaactaccag atgatttagt taaaagaaag cttggaataa gtgataatac tacaaatata
    20341 tataagattg tcagacttag atatactgat ggagagccat ttggattaga aatagtatat
    20401 ctatcagaag aaatgtgtaa gggtctaaca aaagggatat tggataattc ttcattatat
    20461 agggttttaa atgaaaaata tggctataag atacaaaaag ctgaacaggt aatggagcca
    20521 gtaatacttt cagatgaaga aagtaagctt cttgaaacag atgaaggtgc tttagctctt
    20581 aaacttcata gaaattccta taatagagaa ggtagaccta tagaatatac catatcaata
    20641 tttagaacag ataaatatca atatgaaata gtattgagtg agtaggtgaa taatatgaaa
    20701 tgtttaatta atggaaagat aatattaaaa aatcaaatct tagaaaataa agtattagta
    20761 tttgatgaaa aaataattga tatagctgat agtgtaccaa aagattgtga agttatagat
    20821 gctgatggaa aatatatatc tccagggctt attgatattc atatacatgg aaacatggga
    20881 aaagatacaa tggattcgac tgatgaatct attgaaacaa tttcaaaatc aattatgaga
    20941 catggagtaa catcttttct tccaactaca atgactatgg ataaagaaca tgtttatgat
    21001 gcacttgaag ttattaaaaa agcccaaaat agaaagcttg aaggggcaca agtattagga
    21061 gcacatttag aaggaccttt tataaatgag aattataagg gagcacaaaa tgaaaaattt
    21121 ataataaatt caaaatacga atttataaaa gaatataaag atgttataaa agttataact
    21181 tatgctccag aaaaagatat tgattttgat tttactagag aaataaaaag atgtacagat
    21241 atagttttat ctattggtca tagtaatgca aattatgacc aagctaaaga agctataaat
    21301 ttaggagtaa ctaatgtaac tcacatgttc aatgcaatga ctggtttaaa ccatagagac
    21361 ccaggcgttg ttggtgcagc acttacaact aatgtgtaca gtgagttaat agcagataca
    21421 atccatataa ataaagattt atttcaattt atactaaata ataaaggaaa agaaagactg
    21481 attttaataa cagattctat agaagctggt ggtcttgaag atggcaatta ttctcttgga
    21541 ggtcagaaag tcatagttaa gggtaatgaa gctagacttg aaaatggagc attagctgga
    21601 agtgtgttgt ctttaaataa aatggtattt aacttcttaa ataatacaaa ccttaaagtg
    21661 aatgaagcaa ttaatttagc ttctttaaat ccagcaacat ctttgggaat aaatgacaaa
    21721 aaaggaagct tagaaatagg taaggatgca gatatagctg tatttgatga gaatttagat
    21781 tgtaaaatga cactttgctt gggggaagta gtatataaaa atatatagta tacaaaacaa
    21841 accttaaaga cagtaaaaac aatataaaaa ataataaatt aaacttacat tagtaaaagt
    21901 agtagttaaa atttaggagg aataagatga gagtattagt gtgtaaagat tatgatggaa
    21961 tgagtaaaaa agccgctgaa atgatagcag ctcaaatagt tttaaaacca aattcaatac
    22021 tgggactagc tacaggaagt acaccagtag gtatgtatag agatttagta aaaaaataca
    22081 atgataatat agtagatttt tcagatgtga tgagctttaa tttagatgaa tattataagt
    22141 taccaatatc taatgaccaa agctatgatt attttatgaa agagaatcta tttaatcatg
    22201 taaacataaa gccagaaaat actcatctac caaatggaat ggctgatgat attgaaaaag
    22261 agtgtatgaa ctatgaagct tctatagatg cagctggtgg tatagatgta caagtacttg
    22321 gaataggtcg taatgctcat ataggtttta atgaacctga tactaaattt gcaaaaagaa
    22381 ctcatgtagt tgaacttaca gaatctacaa tagaagctaa tgcaagattc tttaaatcaa
    22441 gagaagatgt tcctaaaaaa gctgtaagta tgggaatagg ttcaatattg aagtctaaga
    22501 aaatattgct tttagcttca ggagaagaaa aagctgatgc agtatataat actgtttacg
    22561 gagatataac tccagaagtt cctggttcta tacttcaact tcataaagac actatagtta
    22621 tagtagatga agcagcagca agtaaattga atccaaaaga ttataaatta gtataagaat
    22681 agaatgtagt ttaaataaaa aatagctatc attgaataat caaatttatt taatgatagc
    22741 tatttttatt taaattagtt attattgttt aaattacttg cttattactt tttacgaaaa
    22801 ctcgttatca agaagtatcc aaataagatg ttaaaaatta ttatgtttag tgggattcta
    22861 tcaagcataa taaaattacc tataattggg atattaccaa ctggaaacat aaagaagaat
    22921 acaggcatca gtgatagtaa aaaagatgga aaacaaagaa gaagtaatct tggaatatca
    22981 atataccaac taccatcctt tttgtattct ctaatcaata attcagttcc tagtaataaa
    23041 ccaataccac cagaggaaat taaattaata aaaatgtatc ttttaaagtc ttgcatataa
    23101 ccagtgtaaa attgagtata gaaatcataa tttaatttaa cggctaaaaa atacataagc
    23161 aatagaatga ttaaaaataa aagatatcta agagcttttt tcattttaaa ttctcccttc
    23221 agaaatactt tatttagttt tattttgttg gaatctgatt ttatcattta gtttaagaaa
    23281 tccataatat tagtgaagaa tgaaataagt attatatatg aataaatatt tttagaatta
    23341 aggtgaattg tagtgcttaa tataactata atattaattg tttgaatatt caataaaaag
    23401 ttcgaaaaat atcaattctg atataaactg aataaataca gttgaatatt aaattattat
    23461 tttatcaaaa atatgcaaag tactacacct tcaaatatca cctaaagtaa ttaaaaggca
    23521 attaaagtga agtagttcaa gtatttctaa aataagttta ataaattcta taataattta
    23581 ttttttgcct taaaattgcc ttaaaatcc
//
LOCUS       DBGJFU010000056        23550 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.056, whole genome shotgun sequence.
ACCESSION   DBGJFU010000056 DBGJFU010000000
VERSION     DBGJFU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.056"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            132..674
                     /locus_tag="ACOIAS_002053"
     CDS             132..674
                     /locus_tag="ACOIAS_002053"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2701765.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            1002..2495
                     /locus_tag="ACOIAS_002054"
     CDS             1002..2495
                     /locus_tag="ACOIAS_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGZ2701766.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYNSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            2569..2802
                     /locus_tag="ACOIAS_002055"
     CDS             2569..2802
                     /locus_tag="ACOIAS_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701767.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            2958..3722
                     /locus_tag="ACOIAS_002056"
     CDS             2958..3722
                     /locus_tag="ACOIAS_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2701768.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4007..7186)
                     /locus_tag="ACOIAS_002057"
     CDS             complement(4007..7186)
                     /locus_tag="ACOIAS_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HGZ2701769.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPREYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            7467..9644
                     /locus_tag="ACOIAS_002058"
     CDS             7467..9644
                     /locus_tag="ACOIAS_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HGZ2701770.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFTNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            9658..10080
                     /locus_tag="ACOIAS_002059"
     CDS             9658..10080
                     /locus_tag="ACOIAS_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2701771.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10488..11291
                     /locus_tag="ACOIAS_002060"
     CDS             10488..11291
                     /locus_tag="ACOIAS_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2701772.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            11284..11952
                     /locus_tag="ACOIAS_002061"
     CDS             11284..11952
                     /locus_tag="ACOIAS_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGZ2701773.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            12188..12850
                     /locus_tag="ACOIAS_002062"
     CDS             12188..12850
                     /locus_tag="ACOIAS_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGZ2701774.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            12910..13707
                     /locus_tag="ACOIAS_002063"
     CDS             12910..13707
                     /locus_tag="ACOIAS_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2701775.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            13840..15015
                     /locus_tag="ACOIAS_002064"
     CDS             13840..15015
                     /locus_tag="ACOIAS_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2701776.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSKLIKEE
                     LNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILEYIV
                     KNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDYLCQL"
     gene            15124..15873
                     /locus_tag="ACOIAS_002065"
     CDS             15124..15873
                     /locus_tag="ACOIAS_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701777.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
     gene            16464..17735
                     /locus_tag="ACOIAS_002066"
                     /pseudo
     CDS             16464..17735
                     /locus_tag="ACOIAS_002066"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
     gene            complement(17899..18603)
                     /locus_tag="ACOIAS_002067"
     CDS             complement(17899..18603)
                     /locus_tag="ACOIAS_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2701778.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYTKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            18768..19493
                     /locus_tag="ACOIAS_002068"
     CDS             18768..19493
                     /locus_tag="ACOIAS_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2701779.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSMSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            19553..21145
                     /gene="hcp"
                     /locus_tag="ACOIAS_002069"
                     /gene_synonym="priS"
     CDS             19553..21145
                     /gene="hcp"
                     /locus_tag="ACOIAS_002069"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGZ2701780.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDICGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            21517..22422
                     /locus_tag="ACOIAS_002070"
     CDS             21517..22422
                     /locus_tag="ACOIAS_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGZ2701781.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            22546..23499
                     /gene="msrA"
                     /locus_tag="ACOIAS_002071"
     CDS             22546..23499
                     /gene="msrA"
                     /locus_tag="ACOIAS_002071"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGZ2701782.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTHIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
BASE COUNT         9098 a         2532 c         3975 g         7945 t
ORIGIN      
        1 caaatatgtt aaaaaaatga taatcacgga gaacttttac cttcttcaat taatatatta
       61 ttattagtaa taactttaaa attatattac tattatataa aaattggctt aaaagaaggt
      121 gaaaaaagta aatgaaaata tgcataacag taggtcatag tatactaaaa ggtggtaaaa
      181 gtacaggtgt aaatggtata gttgatgaat acaattataa taaaaaatta gctcctatgt
      241 tagctgagat gcttataagt caaggtaata cagtagatgt aataatttgt ccagaaaggc
      301 actttatgtc agaaagagaa gaatttttct atagagttcc aaaagtaaac agtggtaaat
      361 acgatttatt agtagagcta catttaaata aagctgatgg aacacaatgt ggtacagaag
      421 tgctttacta tggcactgaa ggattagaat atgctaaaag agtatctaat agacttggag
      481 aattatttga aaatagagga gctaaaaaaa gagaaaattt atatatattg agaaatacaa
      541 atccagttgc aatacagatt gaaagttttt tctgtgataa tgtgaatgat tgtaacaaag
      601 caaatgaatc aggttatgat tatatagcac gattgattac agaaggtata ttgaataaag
      661 atataaatat gtgagattat ggtgatttag gtattgatta atacctttta atattagata
      721 taaataaaaa tacaatgtat tagtataatt taaactttca aaagatagat tagtttgatt
      781 attgacaaga gatacctcct tgaaaatatt gatttatcag tatatttaag gaggtattta
      841 aatataaaaa tttaattaat ttgctactaa actataggtt tatagagtat atatttttat
      901 cttcagtcta ataaataata taaaaatagt agaaccttgt attataataa tataatgtag
      961 aaaaatatat agataagata aatataataa ggtggtaata tatgaaatat acatttgaaa
     1021 aatttaagtt aaatgaaaag atacttaagt cattgaagag tttaggatat aatatacctt
     1081 ctagagtaca aagagaagtt attccaaaat tattaaaagg tcaaaactta gtagttaggt
     1141 caaaaacagg aagtggaaag acagcaagtt ttgcaatacc tttatgcgag aatataaatg
     1201 tagattacaa taatattcaa gctcttatag tggttccaac aagagaatta gccttgcaag
     1261 taaaagatga gatatcagat atagggagac taaagaaggt gagatgtagt gctatatttg
     1321 gtaaacaatc tataaaagac caaatagctg aattgaaaca aagggttcat attgttgtag
     1381 ctactccagg cagaatactt gaccatataa atagaggttc tattaagttg gaaaatgtaa
     1441 agtatttagt aatagatgag gcagataaaa tgtttaataa aggttttgtt gaacaaatgg
     1501 aaaaaatatt attaaattta cctaaggaga aaatagtaag tcttttttca gctacaatag
     1561 atgaagagat aaaatatata tgtgaaaaat atatgctaga ttacagtgtt ataaatatag
     1621 aagaaaatga gagcgatact aatcaaaaaa caagacaaat agatgacaaa ataatcaagg
     1681 caaatggaag agagaagtac atccttttaa aagaattaat atacagcgaa aatccaaaga
     1741 gtgtgataat attttgtaat accaaagaga aagtatctaa attatataac aagatgagta
     1801 aagaaggttt tttaataaga gagttacatg cagatttatc acaagaaaga agaatttttg
     1861 taattaagga ttttaaaaac caaaaattta atgtattagt tagtagtgat gtagcgtcca
     1921 gaggaataca tattgatgat atttctttag taataaatta tgatgtgcca caagataaag
     1981 aaaactatat acatagaata ggtagaactg ggagaaaagg aaatagtgga aaggcaatta
     2041 ctatagtaac tgaaaaggat gaaaaatata tagagaatat agaaacgtat ataggatata
     2101 aaataaatga attaaaagat atagaaaaaa gtagaattac acatggcaaa attttgtttg
     2161 aagaatattc aaaaggaatc ctaaaaaaag tatcaaagag aaaaaaagtt gataaaaatt
     2221 cttacaatag taaagatata aaactaaata tagaaagtga agttgttaaa ttatatttaa
     2281 atgctggtaa gaagaagaaa ataagagttt tagatatagt tggtgcattt agtaatataa
     2341 aaggtataac taatgatgat ataggtgtaa ttgaagttca agatttatgt tcatatgtag
     2401 atatattaaa ttataaggga gatttgatat taaaaaaata taaggagata cctataaaga
     2461 aaaaaatggt taaagtgaaa agagatatac gataatttag tcaatattga tgaattgctt
     2521 tgttatcgaa atgaagttgc aaaaaacaca atagaagttg ttaaggattt gaaagctgaa
     2581 gatatgaaac gaaaggtctc tccaattgac ttagaaagga ttttggtgac tggaggagta
     2641 ataaagcatg aagattctat ttggttgttg gacttttggg ggaataaaga tattgctgga
     2701 attttgctta tgcctcctac tcgtcatgtt atacttcatt tgaatgattg ttgtaaatgg
     2761 aaggaatatt ttcgtacaaa aaagaaattt tacagaagtt aaattataat aaattagaca
     2821 aacaatagat aaattagcaa ggaatgttga ttttatctac attctaagtt atactatttt
     2881 taagtggcta atggaatagt cattttaaat ataagtaata aatatatacc tatagggata
     2941 ttaaaggggg gaaatatatg gcatatttta actataacga caaacagtgc tactataatg
     3001 aaattgggga tggtacacca ttactttttt tacatggaaa tacagcctct tccaagatgt
     3061 ttaatgatat aatcgatttt tataaagatg aatataaagt aatattaatt gattttgtag
     3121 gtcatggaaa atctcaaatg gtcgataaat tttctgctga cttatggttt gatgaggcta
     3181 tgcaagtgat atgtttttta gaagcaatga actataaaaa agtaaacata attggtagta
     3241 gtggaggagc tcttgttgca ttaaatgttg cacttgagag acctgattta gtgaataagg
     3301 taattgcaga tagttttgaa ggagaagttc ctctagagtc atttgtacaa aatgtgaaaa
     3361 tagagcgtga agcatctaag caagatgatg gtgcaaaagc tttttatatt tataatcaag
     3421 gagaaaactg ggaaaaagtt gtagataatg atacagaagc tatttttgaa cattataaaa
     3481 ctataggaaa attttttcat aagcctctag aaactatgca accagaggtg cttttaacag
     3541 gaagtagaga agatgagttt gtatctctaa tatctagtga tttttttgaa aatacttttt
     3601 cattacttct tgaaaagatt aaaaatggta aaatgtattt gtttgataaa ggtggtcatc
     3661 ctgcaatttt atcgaatgga ttagattttt caaatgtagc aaagaaattt ttagaggaat
     3721 aatttaacca aaatttataa taatgaaaac tgtaaaatgg attgcaaaac ttacaaaagt
     3781 gagtgtacat atacttcatt attatgatga aatagtattg ttaaaacaaa atattctaat
     3841 agtcaagtta ttacatttgt atatgcaata tgtatagaaa gagatatcat tagattgtaa
     3901 agttgggatt aaataacaat attaaatttt atttattcag atattttagc ttaactatat
     3961 aaatgttaga acaaatttat atagttaagc tgtaggatta aataatttag ttaaataagt
     4021 ttattatctc ctcatcagat agacttttta atacaccttc attagaaagt tctccattta
     4081 taaattgatt tataagctct tttttacttt cttgtaattt tattattttt tcttctatag
     4141 tccctttagc aatcaatttt ataacttgta ctgaattttt ttgaccaaat ctatgagctc
     4201 tatcactagc ttgattttct actgatggat tccaccatgg gtcaaagtga attaccatgt
     4261 cagcagatgt aagatttaaa ccagttccgc ctgcttttaa agatattaaa aaaacttttt
     4321 tatccatact attattgaac tcatctacaa gttcaagtct ttctttggca ttagttttac
     4381 catctatata atggtattta attttatact tatcaagttc cttacttatg ttcttaagca
     4441 cagaagtaaa ttgtgaaaac aataatattt tgtggttttc gtttatacta tccctaagta
     4501 tttctaaaca tgtttcaatt tttgaacttt ttttattgta atcttttact actatacttg
     4561 ggtctaaaca aagttgtctt agtttagtta aataagaaaa tataactatt ttatctttct
     4621 ttagattttt atctttcatt ttatcttgta tatcttttga atatacacta tatatctttt
     4681 tctgctcttt attcaattca acaaaaaagt ttttttcaat cttatctgga agttctttca
     4741 taacttgttt tttacttctt ctaagaataa aaggttttat caattttttc aggtttttaa
     4801 cattatcttc tttatttata aacaattcct gaaactttgc tttactatat agatagccag
     4861 gcattataaa atcaaatata gaccataatt ctagtagatt attttccata ggtgtaccag
     4921 tgagtgcaaa tttattttct gcatttatac tttttacaga ctcagttact aaagctactg
     4981 gattttttat attttgagct tcatctaata tacaataatc aaactttatt tcactatatt
     5041 tttctaaatc atttctaaga gtaccatatg ttgtaagtat tacatcaaaa ttttctaatt
     5101 ccataaaaca tttttctcta tctcttttat ttccatgtat caataaaact tttatatctg
     5161 gagcaaactt ttcaaattca tttttccaat tataaatgag tgatgtcggt gtaacaataa
     5221 gacttttttt attagataat gataataaaa atgcaatagt ctgtattgtt ttaccaagac
     5281 ccatttcatc agctaatata ccaccaaatt tataataatc taaaacttta aaccaattga
     5341 ggccatttat ttgatagtca cgaaggtttg catttaaatt tgcgggaatt tctaaattta
     5401 tgctatcaaa atttctaaat ttttcacata tactgtctag ttctttttta ccatctataa
     5461 actttaaatt tttactacgt attacatcat ttatatatag agctttattt ttatgaattt
     5521 tattattttt tgatttatcc attatatcta gattttcaac taattcaaaa aaatcttttg
     5581 tttctctttc tgataaatct ataaagtttc catctttaag tttataaaaa gtacgttttt
     5641 cttgaaaagc tttcaaaata tccttgtact ctcttggatt tatatcagat atattaaaat
     5701 taaattctag gtaattctgg ttagtctgat tgattgaagc ttgaattgat gaagcgttat
     5761 atattttttt caatttaaac ttctcagaat aatatatatc tccaaattcc tttagagatt
     5821 ctaattgtaa atctaaaaat gtaaataggt catggtcagt tcctttaaac acataatgat
     5881 tattttcctt tgtaaattgt gaagaagtaa gtttattttc aatagtagct tccttttgta
     5941 tgttggggag cacaaatttt ttatcttcat catcatatat atactttaca tcacaacata
     6001 tgttgccatc aatcatatca aagtagaact cagatttaaa atcttttgtt atattatttt
     6061 ttatgttatc atctaaatga acttcttgag atatagattt taatctagga actagcaatc
     6121 ctaaaacatc cttagaatcc tctttatcaa attttaactc tttagtcttt gataatattt
     6181 gatatatttt tgaataatca tgagcttttt tatgtgataa taaatagata ttgccatcat
     6241 ataaaagtac atcatttttt ttagtaagtg gaacaggtag tgaatcttta gaatataaga
     6301 ctattttatc ttctatattt ttaaaatcaa accttacagg tagagtatca tttaatatct
     6361 ttggatgata ttcctcttta gaaaaaataa gggtaaaatc cttgtactta atagcattta
     6421 aaaattgttt taaagagctt gcatttatct ccataaattt ttttgaatgt gatgaagaaa
     6481 agttgtatga aagatttgtc ccatattctt ctataatatc tactaacttc tcatcttcat
     6541 cagcaaaata atgagtgttg gggttgtaag taaatttctt cccaaaatct aattcatatc
     6601 cattaagttt tgaatatgca aattcagtaa tgttttttaa aatgtacatt ttatcaattc
     6661 ctattttaaa ttcagcaatt gctttatttg aatccacatt tatcaataca tctaaattaa
     6721 ccctttctct tttagtattg tctaacaaag aaagtaattg cttgcttaaa tctaagctgg
     6781 ttttagaatt tgtattagaa agcttatcct tatctgcacc taatatatct attagaatag
     6841 gaggaacatc ttttttattt tttatttgtt ttaagctttt tatcacaata gcaactatat
     6901 gcttacatat aaaaatactt ttgccctttg attgacagtc agcgcaacta caatcatttt
     6961 caattatctt aaagtgcttt atatctatag ttattgaatt aaaataaaag ctataattat
     7021 cttcagaatt gactccacca cgaaatgtca acaggttatt ttgtaggtca tcaatatcta
     7081 tgtctatata atcaactaat tgattagtat aatattttac acctcttgac catcttgatg
     7141 gttctgtatt tcttcttatt agtttatata tcatatctat tttcatatta ttcaccttac
     7201 ctcactgtat atattataat ctaaatgcat tcaatttaca ctctaaattt aagactaaaa
     7261 ttaaggcaga tatgaatagc ttttatctat cacaatatat gtgtaaataa caatttacta
     7321 ttagattttt attgaattat cttataaact atgaatgaaa aataattata taaatagatt
     7381 attaataaat ataaaatctt attattaaaa atattcttag aaattacact atataaaata
     7441 taaagtatga tatttgaata aaaagaatgt ataataagta taataatttg tatttattat
     7501 tgggaaagga aaatgttaat atggatagtt atagacaact taaagctaaa ataccctgta
     7561 aggaaacagg tatagaaata aagaggactt tatgtgatat ttgttctcca ggagtacatt
     7621 gtggaattga tgcatatgtt aaagatggta aagtaataaa aatagaaggt acaaaagaac
     7681 atccaatgaa taaagggttg ttatgtacca aaggtctttg caatagagaa tatatctata
     7741 gagaagatag aataaaaaca cctctaaaaa gaattggaaa aagaggtgaa ggaaaattta
     7801 aaccaatatc atgggatgaa gcttatagag aaatagcaga aaaattaaat agagtaaaat
     7861 ctgagtatgg agcaaagcat gtagcttttt ttacgggtta tggaaagtgg tatagacctt
     7921 ttttacaaag gtttgcatct tcatttggtt cacctaatta tggtacagaa tctagttcat
     7981 gttcaacatc agcacagatg gctggaaatg ttgctactgg aagaaacttt acaatgcaag
     8041 atatggagaa tgcaaaaact tatttagggt ggggatttaa tccatactac tctaaatttt
     8101 taaatagtgt gaatctaata aaattaaaag aaaaaggtct aaaatttata attgtagacc
     8161 ctagaataac accgacaact caaaaactag cagatttaca tttaagaatt aaacctggaa
     8221 ctgatggagc tttagctctt ggaatggcga atctatttat acaaaaggat ttaatagata
     8281 gagaatacat agaaaagcat gtacatggat ttaatgaata taaagaatat gtaaaattat
     8341 ttgatttaga tactgtaagt agaattacag gagtttctaa agataatata gtaaaagctg
     8401 tcgagatgtt gtcacaaggt ccattttgta taaatgaaag tgcagctcca atagttcacc
     8461 atcaaaatgg atttcaaaat tatagagcaa tgatgtcttt atccgctata acaggtaatt
     8521 atgatagaat aggtggaaac ataccaaaac aagattactt tgctggtaag ataggtggat
     8581 tccaaacact agaaaaagag tttgtaaaag aagttctacc taaaaatata aatgaaagaa
     8641 ttggtggaga aagatttcct ctatggaatg aaatggtgac tcaatttcaa gctatggatt
     8701 tacctaggca aatagaagaa aagacaccat atgaaataag agctttgttt tgtcatggga
     8761 taaatgctag aatgtttact aattcaggga gactttttaa agcgttgaat agtgtagatt
     8821 tttttgtgaa tatagattta tttatgacag atacatcaaa atatgcagat atacttttac
     8881 ctgcatgtac atcatttgag agagaagaat gtaagagtta tccaggaggg tatattacat
     8941 ataccaaaaa agttattgat aaattatacg attcaaaatc agatgtagaa atactttcag
     9001 atttagcaaa tgctatgaat atagatgatg atttattaaa agcaggttat gaagcaagta
     9061 taagacatat gtttaaaaat acatgtgtag atgtagataa acttaaagaa agtgatttac
     9121 cattaaaatg taaagatttt aagccatata tagtaggttc atatacaaga gaagggtatg
     9181 atactagcac ttctaggttt gaattaaaat ctacaattat tgaaaaatat aaagattttg
     9241 ggctggatgc actaccaaca tatagaggat ttaatgataa tatagatgat gaatatactc
     9301 atttattgac atcaggagca agaatttcaa atgcactaca ttcaagactt catgacatca
     9361 aactttcaag aatattaaaa aatgaagcta gttgtgatat ttctagctta gatgcaaaag
     9421 aattgggtgt tatcagtgga gattatataa aaataactac taagatagga agtttggatg
     9481 taaaagtcaa tgttactgat agagtcttat ataagaatat acatatgtat catggatata
     9541 gagaagctga tataaatagt attatttgtg ataattttga cccttatagt ggattccctg
     9601 cgtatagaag ttgtaagtgt aagattgaaa aaatgaatag ataaatggag agatataatg
     9661 aaaagtttat ttaaatttaa taaagaatta tgtacagcat gtggagcatg tttgcttgca
     9721 tgtatagacc aaaatgataa taatatagac aatggtgaac tttttagaca tgtatttaca
     9781 atagaggaag gtgaaaatat agaatatttt tcattagcat gtatacattg taatgagcca
     9841 aaatgtttag gtgtatgtcc taaaaattgt tttaaaaaag aagaaggatt cattgtatta
     9901 gacaatcaaa actgtatagg gtgtaaactt tgtgaaaaag cttgtgaata tggagcctta
     9961 atttatggaa atgatggtaa ggcaaataag tgtaatggtt gtattgagag atataagtgt
    10021 ggtatggatt caccttgtgt aatggtatgt cctacaggag cattaaaatt taataaataa
    10081 attaaatata gagaatgact tatacttagg tataagatat ttttacaaaa agctgtattg
    10141 agtttgttat aatgaaaact caatatagct tttttatgga tatagttgga tactataatg
    10201 tgattcttag taatagtttt ttgctattgg acaaagactg taaaacatat taaacttatg
    10261 ttgttcaaag tcgaagtata atataataac tagaaaattg gtttatagtg ctataaaagc
    10321 aaatttagta aggtataaaa tcagaggcaa tcctttacta taatatcaat atttgggagt
    10381 tctgttattg cagagctatt aaacaattat aatggtacaa gtgattcaac ctttgaaaat
    10441 gtattatata aaaaattaaa ataagattaa aaaggagaga ataaattatg aaaaaactta
    10501 aattaattat tatgtttgcc ttggtatcag cactgttagt tacaggatgt tcatcaggaa
    10561 ataataaaaa taaagaagaa caaaaagtta taagagttgc aacatcagga acatattacc
    10621 cttttgcttt taaagataaa gataaactta aagggtttga agtagatttt tgggatgaat
    10681 ttgctaaacg aactaattgt aaagttgaat gggtattaac tgattttagt ggattatttg
    10741 gattattaga agctgataaa gcagatgttg tatctgctca acttactgca acacctgaaa
    10801 gagagaaaaa atatgctttt tcagatgctt ataattatag tggaacaaac attattgtta
    10861 gagaagatga tactagtata aaatcaatag atgatttagt tggcaagaaa gtaggtgtgg
    10921 gtactggagc agtagcaaat gaaatattaa aagaaaaata cccaaatgat gaaataaaaa
    10981 tagtaaatta ttcaagtgcg actcttaaag ggaattatca agacctagaa atgggaagat
    11041 tagatgcagt agttgcgcag gatgttgagg ctttaatagc tataaaagaa caaaaattaa
    11101 atcttaaaat gatagaacct ccaattcaat ttggtgcctg ttcttttgcg cttcaaaaaa
    11161 atgaaaaagg tgaaaaatgg acaaaagaaa ttaacgaagt aattgaagag atgtataaag
    11221 atgggacttt gactaaaata tctgaaaact ggctaggaaa agacattact aaggagccta
    11281 ttaatgagta attttaattt tgaattttgt ctaagtatat ttccattttt gattaaagca
    11341 ttgggaataa caatactaat atctattgtt gcattttttt tatcaatatt attaggtgta
    11401 atacttgcct ttttcacaca gacaaaatca aaagttttac aatacataac aagagtgtat
    11461 gtttcattct ttagaggaac accactttta attcaaatat ttttctttta ttttggattt
    11521 ccaatgatat tttcttttat gaaagattgt tcaccttatt tagcaatagt aatttgttta
    11581 ggtgtaaatt catctgcata tatggctgaa attatacgag catctattga gagtgttgat
    11641 aaaggacaac aggaagcatg ttatgctttt gggttaagta aatatcagtc aatggttaaa
    11701 ataattctac ctcaagcagc tacatctgca gtacctccta ttgtaagctg tacactggac
    11761 ataattaaag gttcttcttt aggtttaact ataggaatac aagatttaat gggagtagct
    11821 caagtagagg ctgcttcaag ttttaaatat tttgagatat atatgataac gatattaatt
    11881 tactgggcag tatctataat attgagtaat atacaaagca aagttgaaaa aagaataaga
    11941 agagcatatt agatttcaat atttaaaaat gggatgatat agatatatta ttacttgtaa
    12001 ataaattttt aattttagaa aaataaactt agtaatctaa attattgaga aaatatagta
    12061 tcaaaatgga ctgtataatc aagttaaaaa atagtttttc tattgaaaaa ataaataaaa
    12121 ttctattgta atttaatatg cgataaagtt ttataaaaaa caacattaag ctagggggac
    12181 ttattttatg gcttttaata tgaaatattt tttagaatta tttccaacaa taatgaaatc
    12241 gttcaatgta actgtgattt tggcaataag ttcattagta ttttcattaa ttataggtac
    12301 aatagtagcg ttatcatctt attacaaagt gaaagtattg aatcaaatat gtaaagtata
    12361 tgtatctatt tttagaggta cacctcttat gccacagtta ttctttttat attttggact
    12421 agcatatatg agtgattttg ttaaaaatat ggacccagta tttgcaacat ctatagtact
    12481 tagtttaaat atgggagctt atatgtctga aacgataagg gcatctatac tttcaattga
    12541 tacaggtcag atagaagcag catatgcaat gggattaaca aatttacaaa caatgaaaag
    12601 gatagtgata ccacaggccg taagagttgc actgccatca ttattcaata attttataga
    12661 tttaattaaa gggtcttctg tagcttttgt tgttggagta aatgatataa tgggtgctgc
    12721 caaatcacaa ggagcgttga gttttaagtt ttttgaagtg tatggagcag ttatggtaat
    12781 ttactggtgt ataataactc ctttaggttg ggggcaaaaa aaattagaaa agaaaataag
    12841 caattattaa gttattaaga aaatacataa aagtattttt atataaaaaa tggtgtgagg
    12901 agaagagata tgataaaaag aaatattata aaatgtttag tggcagtttt atcgttaacg
    12961 tttgtaatta ctggttgttc aaaaaaagag gaagtaaaga atgaaaaagt tataactgta
    13021 gcttcatcta attcagctga cccatattct tatgttgata atggtgagca taagggtttt
    13081 gaagttgaca tgtggaaaga gattggtaaa agaagtggat ataagataaa aatggaacca
    13141 acaggtttta gttctatatt tggtatgtta gattctggta aagttgatgt ggcagccaac
    13201 ttctttggaa tgtctaaaga aagattagaa aaatacgatg cttctatacc ttatggttct
    13261 gattctgtgt gtatagctgt aaaagatgga aataacgaaa ttaataagct tgaagattta
    13321 aagggaaaag ttgtagcagt ttcagaaggt tctcaaggtc aacaggttgc aactagtgtt
    13381 aataaagata atttgtttca agaaaaggtt tacggtgatg gaactagtgg tcttcaagat
    13441 ttagatttgg gaagagttag tgcatggatt gatgcagagc ttactattgt gggagacgct
    13501 aaaaaagcta atatgaaagt aagagtatta gataaaaaat tatcagctac taatatagca
    13561 tacttcttta agaaaaacga tgataaatct aaaacgataa aagaagatgt aaacaaagca
    13621 atagaagaaa tgcttgcaga tggaacagtg aaaaaaatat ctgaaaaatg gtttggaatg
    13681 gatgtaagtg ctgatataca aaaataaata ctttaaaatt aaaatgtaaa actgtgataa
    13741 ctatagataa gtatactaaa tttatctata gttatctgtg tattacaatg tatttagatt
    13801 agtctttata aatcaaaatg agttatggag gtattgcaaa tgattgaaac taaaataaaa
    13861 tccattgcac taaaaaatag agaaaaaatt ataaaaataa gacgacagat acacagtaat
    13921 ccagagttag catttaaaga atataaaaca tctaaactta taaaagaaga gctaaataaa
    13981 cttaatatag aatacataaa tattgctggt acaggagtat tagctactat aaaaggaaaa
    14041 aataatggtg gaaaaactat attactcaga gcggatatgg atgctcttcc aatcaaagag
    14101 gaaaatgatt tagaatttaa atcaataaat gataatatgc atgcttgtgg gcatgatgca
    14161 catgtttcat ggttgttagg aacagcaatg attttaaatg acataaaaga agaattgaat
    14221 ggaaatgtaa aattactatt tcaacctggc gaagaaaaag gtggttctga tattgtaatt
    14281 aaagaaaatg tacttgaagg tgtggatgca ttagcgactg gtcattgttg gccaactatt
    14341 gagtcaggaa aaattggaat cgctagaaat tgtgctatgg cagcgactaa tacttttgaa
    14401 ataacaataa ttggcaaggg tgggcatgga gcagagcctc ataattgtat agacccaatt
    14461 gcagttggaa atgcagtata ttcatccatt caacagatag tttcaagaaa aattgaccca
    14521 gttgtacctg tagtagtgtc tgtttgttct tttaattctg gtgtttctaa aaatataata
    14581 ccagatgtat gtacacttca agggactatt agagcgataa gcaaggaaca agtaattgaa
    14641 gtaagtaaaa ttctagaata tatagtaaaa aatgtatgta aagcacatgg agcagattgc
    14701 aaatttgaaa agagtacagg aggagatgct gttataaatg acaaaaacat gatagagata
    14761 ggaagagaaa gtgcttgcaa aatacttgga gctgaaaatg ttgaaataat tgattttcct
    14821 gcaatgacag gtgaggattt tgctatttat atgaaagaaa aacctggttt atttatgtat
    14881 ataggtgtag gagataaaga aaaaaatata aattacagat tgcatagtaa caagtttaat
    14941 atagatgaaa aatgtcttag tatagcacct tcattatttt cacaacttac agtagattat
    15001 ttatgtcaac tatagagtac attcataaaa atatatttag cctattttta aatttaatgc
    15061 tagtatatta attttgatgt agaacatatt atttattata tttatttaaa aaggtggata
    15121 acaatgataa aagtagaaaa tttacataaa agttttaaag ggacagaagt tcttaaaggt
    15181 ataaatttag agataagtca aagtgaagta gtagctatac ttggatcatc tggaacaggt
    15241 aaatctacgt tacttagatg tttaaattat ttagttgagc ctacaaaagg aaagataatg
    15301 attggtgatg taaaagtaga tgtagaaaaa gcaacaaaaa aagatataac caatttaaga
    15361 aaacatacat ctatggtgtt tcaaaattat aacttattta aaaataaaac agcattagaa
    15421 aatgtaatgg aggcattgat agtagttcat aaaaagagta agaaagaagc tagagaaata
    15481 tctttaaaat taatagataa agtaggtatg ttggatagga aagattttta tccatcaaag
    15541 ttgtctgggg gacagcaaca aaggataggt atagcgagag cgatggcagt aaatccaaat
    15601 gtaatattat ttgatgagcc tacatctgca cttgacccag aacttgttgg agaagtttta
    15661 agcgttataa aatctcttgc aaaagaaggt ataacgatgc ttattgtaac tcacgaaata
    15721 aattttgcaa aagaagttgc tgataaaatt atatttatgg ataatggtgt aatagctgaa
    15781 gaaggaactc ctgatgaaat atttaataat cctaaaaatg aaagaacagc aaaattttta
    15841 aatatcatta ggaaagaaga agtatttagt taaaagtgta taaattaagc attagaagat
    15901 atgtaagata cccccattaa atttataagt tagataataa atttaatggg agtatatttt
    15961 tattattaga tttattattt aatttaaaat tagaaatgta actatttgaa aattcttttt
    16021 aaaagtatat caaaatatca aatacataaa aaaataatat ttgacaaagt atcatatgtg
    16081 tttacaaaat caactttatg gagttatata gtataggaat tacaatgaat atactaaaat
    16141 tttatattaa agtacaaagt attaaatttg aaaataatta agtaatatta tatatatttt
    16201 tttatattgt ttgttttatt aaaattaatc tagaaaaaaa gttttaaaag cagaatttta
    16261 ttaatattta tatatagata tgaactatta ataaaacgtt aaaaatacaa aaaaactata
    16321 gaaattgaca tatattccag tatttgctaa agtagctgtt ggggaaaatt acaaaaatta
    16381 ttaatgcgca ttagataatt tactttaatt tttttagcat ttggggtaat ttataatata
    16441 aagcattaga aaggaaaata taaatgaggg actttttaaa aaattataag tcatcaatta
    16501 tacttttagc atgtactata gtaggggcga tagtaggatt agtacttggt ccaaaagctt
    16561 caatactaaa accatttgga gatttattca taaatgctct attaatgata gtagtaccat
    16621 tagtttttct tagtgtttct tcaggtgttg ctagtataaa aaatatggaa cgatttggaa
    16681 aaataatgaa gggtacagct atagtattta tgggaacaac acttgtaatt gcatttttag
    16741 gtattacatc tgcattatta tttaatccgt ctaaaggatt agatgtatca agtgtaaaaa
    16801 atttaatggt gactcaagat gcagaaactt tagaatcaaa aggaacgtta ttagaccaag
    16861 tagtatcaag tattacagtt aaaaattttg gagatttatt ttccagtagt aatttactac
    16921 aattagttat atttgcagtt ttaattggaa cagcaatagc tatgatggga gataggggaa
    16981 atacaataag aaagctttta tcagaaggaa cggaagcaat attaaaagta gtaagtatat
    17041 tcatgcttta tgctcctata gggctaggct gttatacagc tacaataatt ggggaacttg
    17101 gaagtaaaat aattcaagga tatgtaaggg tgtttgtaat ttattcacta acagcagcca
    17161 tgtatttctt attattttac agtctgtatg cttttttatc tgcaggaaaa caagggttta
    17221 gacaattctg gagaaatgct ataaaaccaa tagcaacggc actagctact tcttcaagtt
    17281 cagcatgtat accatcaaat ttagaagcta gtaaaaatat aggagttcct gatgatatat
    17341 cagaaacagt tatacctctt ggaataaata tacataaaga tgggtcagtt ttagcagtaa
    17401 taataaaagt ggcattttta tcaggtattt taggtagtgg aatggcaaat ccagcaacga
    17461 taggtcaatt atttataact gtatttgttg ttggagtatt tatggctcct gtagcaggtg
    17521 gctgacttat tggtgagatg ctcgtggtta gcatcttcgg ttttccagtg gaggcaattc
    17581 cactgttaac agttataact actataactg atattcctaa tactatatta aatacaactg
    17641 gaaatacagt atcatcaatg ttagtttcaa gattagtaga aggaaaagat tggcttatag
    17701 ataaaacagc aattactaca aagaaaataa gctaattgta cacttaatta ataaagttaa
    17761 taggtatctt aattttaata aattagatac ctattaactt tttaagtaaa aatataaatt
    17821 taagattcaa atatgatatt tataatttga atcaactaat atagtatata ttgaaataaa
    17881 tatgtttaaa tatctatttt attttttaaa ataaaagatt tcaaatttat ttttgttaaa
    17941 agataacaag ccttcatctc gcatcttgcc aagctctctt gacatagaac ttctgtcaac
    18001 acataaaaaa tcagccattt catttcttga aaagggaatt gtaaactggt ggctacctgt
    18061 ttgttctatc tgttttgaaa aatacataag caatttctcg cgagttgttt tacgagaaag
    18121 taattctatt ttattgtgaa gtaaaatgtt tttttcagct attaattgta gcatattttc
    18181 aacaagccta ttatgaaata cacatgcaga tgaacaaata ccagttacac ttttaaactt
    18241 tatgaataaa acttcgcaag aagatgttgt ggttactgta acattacttt tatgtagtct
    18301 agtacatgcg aatacctctc caaataaatc tccaggcaag agttcagcta caatattcct
    18361 attcccttcg atatcttcct ttattatatg agcacttcct ttaagcataa ttccaataga
    18421 agtaacttca cttccaacag agaatattat actatctttt gtgtaagtct tatctacaac
    18481 acctaaacat tccaacattt cttcaagttc atcactttta attccttcaa atagaggaat
    18541 ttttttaagt aggtctatat gcttttctaa aaattttttc aaataatcac ttccttgttg
    18601 catatgcaac cgataatttg ttttcattgt actatactaa gtttataaaa gcaaggggat
    18661 ataaaaatcc tactacaaga aatttgaaat aaagaaatat aggtattaaa aatatatata
    18721 taagaaatca atactttaaa ttaaaaaaat tttatgaggt gatataaatg atgagaaaaa
    18781 taattaaaat tgatgaggaa aagtgtaatg gttgtggact atgtgttgaa gcttgtcatg
    18841 aagaagctat aggaatggta aatggaaaag caaaactttt aagagatgac tattgtgatg
    18901 ggctgggaga ttgtctgcca acttgtccaa caaatgcgat ttcatttgag tatagagaag
    18961 cagcagaata tgatgaagct gcagtaaaag caaatatgga agcaaagaaa gcacaaaaga
    19021 aaactttagc atgtggatgt ccaggttctc agtctaagtc aataaataga gaggtttcaa
    19081 atagtatgtc tataagcaat gatatagtag agataaaagg ttctcaactt aatcaatggc
    19141 cagtacaaat aaaattagta ccaactaatg catcatattt aaagaatgca agtcttttaa
    19201 tagcagcaga ctgtacagcg tatgcatatg gaaattttca taataaattt atgaaaaata
    19261 aggttacatt aatcggatgc cctaaacttg atgaagttga ctatgctgaa aaacttacag
    19321 agattttaaa agaaaatgat attaaaaata tagtagtaac acgtatggaa gttccttgct
    19381 gtggtggtat agtaaatgct gttaagacag ctctacaaaa tagtggaaaa atgataccat
    19441 ggcaaatagt tactatatct gtagatggaa aaattgtaga tggagaaatt taattaagtg
    19501 aattatagaa aaatataata aattttaata aataaaaagg gaggaataaa aaatggaaaa
    19561 aatgttttgt tttcaatgtg aacaaacagc aggaggtaaa ggttgtacta taaatggtgt
    19621 ctgtggtaaa aaggggggta cagctaactt acaagacgaa cttacaggag tgttgattgg
    19681 tctttcaaga gcaactattg gaaataagaa tagaccaaca agtgaaacag ataaaattat
    19741 gatagagggg ctattcacta ctataacgaa tgtaagtttt gatgatgaag ctataaaaag
    19801 acaaattgaa aaaactgagg tagaaaaaag taagcttgta cctagatgtt cagattgttc
    19861 aacaacttgt aatagaaatg ataactatga tatgaaggat ttatggaatg ataatgagga
    19921 catacgttca ttaaaatcat taatcttatt tggattaaga ggtatggctg cctatgcata
    19981 tcatgcaatg gtacttggtt atacagacaa agacgtaaat gaatttttct atgaaggatt
    20041 aatagcagtt ggaggagact tgtctattga cgaactttta ggtctagtta tgaaaactgg
    20101 agaaataaac ttaaaatgta tggaattgct tgatagagca aatactgaaa cttatggaac
    20161 accagaaccg actcaggtat ctatgaaaat cgagaaaggg ccatttattg tagttacagg
    20221 acatgacctt tatgatttac acaaattatt agaacaaacg aaagataagg gtataaatat
    20281 atatactcat ggtgagttat taccagcaca tgcatatcca aaactaaaag aatataaaca
    20341 tctaaaaggt aattttggta cagcatggca aaaccagcaa aaagaatttg ataatattcc
    20401 tgctccaata ttatttacaa caaactgttt aatgcctgtt aaagatagtt atagagatag
    20461 agtatttact acagaagtag ttgcttatcc aggtatggtt catattgatg aagataaaga
    20521 tttttctagt gtaatagaaa aagctcttga acttggagga tatagagaag atgttcatat
    20581 gactggtatt aatggaggag atacattaac tactggtttt gcaagaggta cagtttcatc
    20641 tgtacaagat aaagtagtag atgctgtaaa gagtggagct ataagacact ttttattggt
    20701 tggaggttgt gatggtgcaa aacctggaag aaattattac actgaatttg taaaacaagc
    20761 tcctaaagac actgttatat tgacacttgc atgtggaaaa tatagattta atgatttaga
    20821 ccttggggat atatgtggct tacctcgttt aatggatatg ggacaatgta atgatgctta
    20881 tagtgcaatt aaagtggcag tttcattggc aaatgctttt gaatgtggag taaatgattt
    20941 gcctcttagc atggtactgt catggtatga gcagaaagct gtttgtatac ttcttacact
    21001 acttcattta ggtatcaaaa atatctatat agggccttct ttgccagcat tcttatctaa
    21061 gaatgtatta gatatattgg ttgataaatt tgcattgaca cctatatcta ctcctgaaga
    21121 agatatgaag aagattttag gataaataaa catatctata cttaaagata tttatagtta
    21181 aaatattttt aagtatgcca caattaagat aaggggttaa acttaaaatt tgattaaaaa
    21241 atcaattttg agatgaccct ttatttattt tttgtaataa ttgaaaaata aacctgcttt
    21301 cagtatattc tttttaagaa tctttgtatt aaatttataa tacttagata tataatatct
    21361 tatatatttt attttaagta ttaaaggttt gaatgtaact taaatattaa aagaggaaca
    21421 ttgtaatatg taacttgcat aaaatatata tactatagtg aaattatttt gattaaaatt
    21481 taaaatataa ggtgttttat gtgggggtat atgtatatga aaaattctaa ttttaatata
    21541 ttggcatttg atggtggtgg attaaaaggg gcattgagta tatccatact tgagaggata
    21601 gtaaaagagt atccaaatct attaaacaat ataaatatgt ttggaggtac atctacaggg
    21661 agtctaattg cactagggct tgcgtatggt gtaagcccta aagagataaa agaactttac
    21721 tcaattgaaa attcaaagta tatttttaat aaaagttatg cagaaatact cagaccaaag
    21781 tatgaaaaca aaaacttaaa agaagtttta ttaagtatat ttccagagga actagaatta
    21841 aaagatttaa ataaattagt aatgatacca agtttttata taggaaatga ggaaaatgca
    21901 tggaaaccag tgttttataa caatatgcca aattctttta caaagactag caaagttgta
    21961 gatgtagcaa tggcaagtag tgctgctccg gtattttttc caacatataa tcgtcatgtt
    22021 gatggaggaa taatagcaac tgacccgagt ttagcgtgta taattcatgc cattgacagt
    22081 ggatttaaac ttaagaatac aagacttttt tcaataggga ctggatatgt atataacagt
    22141 ataaaggcag atactactga atggggagct attgactgga taattaataa agaacccgat
    22201 ttaccaatta tatctattac cttagaagga aattctcaaa tgagtcagtt attttctcaa
    22261 aaacttcttg gagataatta ctatagatta aatcctaaga tggaaaaaga tgtagctatg
    22321 gatgattgtg atgcattaga atatctaatg tctttaggag aaaattgtga tataaaagat
    22381 gcttttggat ggataaagaa aaagagcttt tatagaaatt agattcaatt tgcaattgta
    22441 tttttacaca tgatttcatt gagtaggtaa aaataataaa atgcagatta ttaagttact
    22501 tcttagggaa taaataaagt ataatttatt taaggagtga tgaaaatgat aaaacttgct
    22561 acttttgcag gtggatgttt ttggtgtatg gtaaaacctt ttgataagta tgatggagtt
    22621 attaaagtag tttctggata tacaggtggt catgttgaaa accctacata tcaagaggta
    22681 tgctcaggaa ctactggtca tttagaagca attcaaataa catatgatga tgatataata
    22741 agttatgatg aacttctaaa tatcttctgg aaacagatta atcctacaga tgatggtgga
    22801 caatttggtg ataggggaac tcaatataag actgctatat tttatcatga tgaagaacaa
    22861 aaaaatatag ctttagagtc aagagaacat ctggaaaata gtaaaatatt tgatagtaaa
    22921 atagtgactg atataattcc agcaactaaa ttctacgaag cagaagaaaa tcatcaggat
    22981 tattataaga aaagaccatt aaactacaat atgtattata aagggtctgg aagatataat
    23041 tttgtaaaaa gccattggga tggaaacaat ataaataggg aagagttaaa aaagacttta
    23101 actcatatac aatttgaagt cactcaaaat gatatgacag aagtaccttt tgaaaatgaa
    23161 tattatgaca ataacaaaga gggaatttat gttgatattg taagtggaga agtgttattt
    23221 tcttcaaaag ataaatttga ttctggatgt gggtggccaa gtttttcaaa gccagttata
    23281 gataaatcta tcattgagag aagtgatttc tcacaaggta tgtacagagt tgaagtaaga
    23341 agtgctaaag gaaattctca tttaggtcat gtatttgatg atggtccaaa agaacttgga
    23401 ggtttaagat attgtataaa ttctgcttct cttaaattta taccaagaga aaatatggaa
    23461 gctttaggat atggagaata tttaaaactt tttgagtaat taggaatatt tattataaac
    23521 ttatctaaaa ggtgaaaata taaaaaaata
//
LOCUS       DBGJFU010000057        23084 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.057, whole genome shotgun sequence.
ACCESSION   DBGJFU010000057 DBGJFU010000000
VERSION     DBGJFU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.057"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            248..1084
                     /locus_tag="ACOIAS_002072"
     CDS             248..1084
                     /locus_tag="ACOIAS_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2701783.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1437..3014
                     /locus_tag="ACOIAS_002073"
     CDS             1437..3014
                     /locus_tag="ACOIAS_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2701784.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGIANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3007..4191
                     /locus_tag="ACOIAS_002074"
     CDS             3007..4191
                     /locus_tag="ACOIAS_002074"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2701785.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4209..4808
                     /locus_tag="ACOIAS_002075"
     CDS             4209..4808
                     /locus_tag="ACOIAS_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2701786.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4832..5302
                     /locus_tag="ACOIAS_002076"
     CDS             4832..5302
                     /locus_tag="ACOIAS_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2701787.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5674..6420
                     /locus_tag="ACOIAS_002077"
     CDS             5674..6420
                     /locus_tag="ACOIAS_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HGZ2701788.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6481..7155
                     /locus_tag="ACOIAS_002078"
     CDS             6481..7155
                     /locus_tag="ACOIAS_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2701789.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            7524..8870
                     /locus_tag="ACOIAS_002079"
     CDS             7524..8870
                     /locus_tag="ACOIAS_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HGZ2701790.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8897..10297
                     /locus_tag="ACOIAS_002080"
     CDS             8897..10297
                     /locus_tag="ACOIAS_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HGZ2701791.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYKNLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10507..11943
                     /locus_tag="ACOIAS_002081"
     CDS             10507..11943
                     /locus_tag="ACOIAS_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HGZ2701792.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            12287..13231
                     /locus_tag="ACOIAS_002082"
     CDS             12287..13231
                     /locus_tag="ACOIAS_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HGZ2701793.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13400..14332
                     /locus_tag="ACOIAS_002083"
     CDS             13400..14332
                     /locus_tag="ACOIAS_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HGZ2701794.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENTLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14437..14904
                     /locus_tag="ACOIAS_002084"
     CDS             14437..14904
                     /locus_tag="ACOIAS_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HGZ2701795.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15148..16002)
                     /locus_tag="ACOIAS_002085"
     CDS             complement(15148..16002)
                     /locus_tag="ACOIAS_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HGZ2701796.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16168..18132
                     /locus_tag="ACOIAS_002086"
     CDS             16168..18132
                     /locus_tag="ACOIAS_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2701797.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            18132..18593
                     /locus_tag="ACOIAS_002087"
     CDS             18132..18593
                     /locus_tag="ACOIAS_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2701798.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18596..18916
                     /locus_tag="ACOIAS_002088"
     CDS             18596..18916
                     /locus_tag="ACOIAS_002088"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGZ2701799.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18932..20032
                     /locus_tag="ACOIAS_002089"
     CDS             18932..20032
                     /locus_tag="ACOIAS_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGZ2701800.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20152..22752
                     /locus_tag="ACOIAS_002090"
     CDS             20152..22752
                     /locus_tag="ACOIAS_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGZ2701801.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYHIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         9079 a         2357 c         4062 g         7586 t
ORIGIN      
        1 ttgtaatata aaatgtcgat tgattttaaa taattaaata tacataagaa ataattaaat
       61 atctttcaat gaaaatatga taaaatatga caaattaatt gatgacaata aaaaatattt
      121 tgttattgta gtattatagg gtaaattgca aaatatatac gaaagtaaaa ttttaaatat
      181 tgacattaaa ttaaatatac tatactatgt aagtgtgatg agagaatttt tttagagggg
      241 atttatagtg aatataaaaa agatatttaa taacaatgta gtagtaagct ccctagaaga
      301 tggcacagaa ataattgtca ctggtgctgg agtaggattt aagaaaaaag taggcgattt
      361 gatagatgaa aatttgattt ctaagaaata ttttgttcaa gacgatcaaa gggacaaata
      421 caatcaaata cttaataaga cctctattga atactttaaa atttcagaag aaattataga
      481 aaaggctaat gaagttttaa atactcaagt gaatgattca ataatattag ccttaactag
      541 tcacatagaa tttgctgtcc aaagagaaaa acaaggtata aaattaccaa acttaatatt
      601 gaatgagaca aaacaacttt atagggaaga atttgaattt ggattatggg caattgatga
      661 aattgaaaaa aggataggaa taaaattgcc tgaagatgaa gcaggatata ttgctattca
      721 cataatcaat ggtttggaaa attctcaaaa agatgaaggt attaatatac tagagttttc
      781 aaaacaagtt attcagataa ttgaagaagt acatggattt aaattggatg taaattcatt
      841 aaattatact agacttataa ctcacttaaa gttttttgtt caaaggattc tccgaaaaga
      901 aacttacaat gatagtgata ttgaagatat gtataagtta gtgaataaaa attacaataa
      961 acctaaagtg tgtactgaag aaattgcagc attggtatta gacagatttg aatataagat
     1021 tagtaaagaa gaagaattat atttaatgat tcacataaat aaaataacaa acagtggttg
     1081 ttaggattgt aactgataaa gcaggcaaaa cctaagtcat tgagcttaac tattagatta
     1141 agttttttga tttaggtttt tttattagat acaatcaaaa tacagacagt gacttttagg
     1201 attgtaactg gttaagcagg caaaacctaa atcattgaat ttaactatta ggttaagttt
     1261 tttgatttag gtttttttat tagatacaat caaatacaga cagtgacttt taggattgta
     1321 actggttgag caggcaaaac ctaaatcatt aaacttaatt attaaattaa gttttttgat
     1381 ttaggttttt ctataaattt gattatgttt ttagataaaa ataggagggg aaaattatga
     1441 aaaaaaataa gttaggttta tggaacttct tccaaatgtt aggaaaaacg tttatgttcc
     1501 caatagcact acttagtgtt tctgggatga tgctaggact tggggcaggt tttacagacc
     1561 ctaagatgat tgaaatggtt ccatttttag ggaatgaata tgttaactta gttttaaact
     1621 tcatgttaac aataggatta tttgcattta acaacttggg agcattattt gcaatggcaa
     1681 ttccactagg attattaaaa aaagaaaaag agtttggtgc tttttcagga ttagtaggat
     1741 ttatagcaat gcatataggt acaaattttt atttaacaag agctgatttg ttagtaccag
     1801 cagaacagat gtcaactaat ggtcaagcta tgattatggg tatacaaaca tataatacaa
     1861 gtgttttagg tggtattgta gcaggattaa ttgtttatgc aatatatgat aaagttagta
     1921 atttaaagat accagaatca ttaggattct acagtggacc aagattagtt ccaattgtta
     1981 gtttaatagt tatgagtata gttggattat taatacctat tgtttggcca ccatttttca
     2041 atgcattcca aagtttagga agatggattt cttcagcagg accattagga tatttttcat
     2101 atgcagtggc agaaagagtt acaattccat ttggactaaa tcatcttgta acatctgtat
     2161 tccgttttac accaataggt ggaactgctg ttattgatgg agaaacgtac tttggtacat
     2221 tgaacatgtt tatggcatat gtagaaaata atcagattat acctcttgat ttagctggta
     2281 aaatggaaca aggtaaattg atgattcaat atggattagc tggtgctgca ttggctatct
     2341 accgtacagc aaaaccagaa aatagaaagg cacttaaagg acttttaata tcaggagtgt
     2401 tgacagttat tataggtggt ataagtgaac caatagagtt tctattctta ttcatatcac
     2461 cagcattatt tgctttccat acatttatga atggtatagc aaacatggta cttccatact
     2521 taggcgtgtt gatgggattc acaggagatt taattgcatt tataagtttt ggtgtgttaa
     2581 gaggaacagc tacaggatgg ccaatagcag tattagtagc agttttatat tttgcaatat
     2641 actattttgt atttaagtgg gcaattatta agtttaatat aaagacccca ggacgtgagg
     2701 ataaagaaat aaaagtatct gaaggaaatg gttcagattt caaaaatttg tctacatata
     2761 aaggacaaca aatgatatct gctttaggtg gtcaatctaa tatattatca ctagataatt
     2821 gtgtaactag attgcgttta aaactacaag atgtatcatt aataaatgag gatgcaatta
     2881 aagaggctgg tggtatagca gttgttaaat tagatgagca tacaatacaa gtaattatag
     2941 gaactcaagt gtattcttta agaaagcaga tggataaagc tatggaaggt catttggagg
     3001 catgtaatga ttaattttga taaaaaagta gaccgattgg gtacatattg tacccagtgg
     3061 gactatgtag aagatagatt tggtaagaag gatttgcttc cttttacaat ttcagatatg
     3121 gatttagagt ttccatttga aataacagag gtgttgcagg aaaggttaaa tcatagagtt
     3181 ttgggataca gtagatggaa acatgatgat tttagaaatt cagttacgaa ttggtatatt
     3241 aaaagattta attgtagcgt tgatagtaac tggatatatt atagtccaag tgtaatgtat
     3301 tcaattagca aacttattga gatgttaagt aatgaaggag atggaatcct tataaatact
     3361 cctgcataca atgcttttta tgatgtgata ataaataaca atcgtaatat tataaagtca
     3421 catcttgtaa ataggaatgg attctattca attgatttta atgattttga gaaaaagtgt
     3481 aaaaaatcta agatatttat tttatgtaat cctcacaatc caactggtag agtatggagt
     3541 gagttagaac ttgttaagat gataggaatt tgtaagaata atgatgtcaa gataatatca
     3601 gatgatatac acatggatat tgtatatgac aatagaatga cacctatctt aaatgttgca
     3661 aaggactatt tagattctgt attcatttgt acatcagcgt ctaaatcttt caatatacca
     3721 gcattgactg ggtcatatgt gattatacca aatgaaggta ttagaaataa atttgaaaat
     3781 atcactagat accgtgattt tgtaaattcg ccagcaattc tagcaatatt agctacaatg
     3841 gtctgttatg atgactgcga atattggtta gatgaactat taaaatacct taaagagaat
     3901 cttaaatata cgattgatta tataaaagat aatttagaac cattgaaatt ggtcatgcca
     3961 gaaggctgtt actttgcttg gatagatttt tccgaactta aaataagtgg tgagaaattc
     4021 caaaaattat tgatggatat tggagaagtt gctattatgt cggggtctgt atatggtgaa
     4081 gaaggtgagt tttatttaag gttaaatgtg gcatgttcaa gagaaaaact tgaagatggt
     4141 ttaaatagga ttaaaaaaac tatggattat attgcaaaaa gaattttata gtaggaaggg
     4201 agatagttat gggatattta aaaacaatgg ttgatgttat gactactgaa atgaatgcca
     4261 taagatgttt aatagatgaa gcaggaatag aatatgaaag tatagttagt gaaattgcaa
     4321 attgtgaagg caaagtaatt tttatgggtg taggaaaatc tgcacatata ggtaagaaat
     4381 tggcagctac atttgctagt actggtacac ctagcttttt tgtacatgca acagaggcag
     4441 tgcatggaga tttaggaatg attgaaagta aggatattac aattttaata tcaaatagtg
     4501 gaaattctat ggaagtggta aactgtataa aatatattaa agcaataggt tctaagacaa
     4561 ttgcatttac ttcaaacaga aattcagtac tggcaaaaga gtgtgattat gctttaatat
     4621 atcctgcaaa ggatgaagca gaccatttaa atctagcacc aacaacatca tctacaatta
     4681 ctttggtttt aggagattct attgcatgtg cattatctaa gagtagtaat tttggttctt
     4741 cagattttta taagtaccat ccaggaggaa gtttaggtga gaagttaaaa acagctaaca
     4801 atggataaaa gcaaaaagga gagtagtttt tatgttcaaa atgtttaaaa aaaaatcaat
     4861 aaaatcaccc atatcaggaa aagtaataga gttatctaaa atacaagatg gtgtattttc
     4921 tcaaaaaatt atgggagagg gtgtagctat agattctaca ggagacattg tatatgctcc
     4981 aatcaatgga aagatagtag tagtagcaga gactaaacat gcatttgtaa tagaattaga
     5041 aaatgggatg gaacttctta ttcatgttgg tttagatact gtaaatttaa aaggcgaagg
     5101 ttttgaggta ttggtgagta tgggagaaca agtgaaggaa ggaacaccaa tgttgaaaat
     5161 taaccgtcct ttgattgaat caaagggtat atcacttatt actccagtta caataactaa
     5221 tcattcagag tacaatatga atacatgtaa tgtaggcaat agtgttgaag gtgggaaaga
     5281 tactgtaatt gagtttaaat aaagaactaa aatacaaggt taatatttat aaatacctat
     5341 aaacttatgt tttataagat tgattgtata gatttgttgt atagatttgt tgtataaaaa
     5401 agaactcttt aattataaat taaggagttc ttttttattt tgtaaatatt gtttagattt
     5461 atatagaaaa acacttagta ataaatagaa taaataagta tttatatagc taaaaatcta
     5521 aatagttttg tttaaatata tttttatatt ttgtaatttc aaattaataa ataattagat
     5581 ttaataaaca aaaaataaaa atgttgatta tattaataaa ctttaatata atatatttat
     5641 gaaaaatgta aactgtgaaa tggaggtagt aacatgaaaa ttgaaaatct ttctattgat
     5701 gatattaaac gaggctatat atttaatgat gatttaaatt gttatagttg cattaaatgt
     5761 acaaaaacat atgaggtagg agaaatatat aaaataggta atagattctt tgaagcttca
     5821 aaagctattg aacttcatat ggaattagaa catagtgatt atttagagca attgcttaat
     5881 agtgattcaa aatacaacac attgactgaa aatcaaaaac aattatttca attatttatt
     5941 agtgatttac cagataagga gatagcaaaa caggttggag tatccacatc tactatacgc
     6001 catcaaaaat ttattttcag agaaaaagca aaacaggcta agctctattt agcactatat
     6061 gaatctgtat ttgaagataa atctaataaa agtagtacta taataccaat ccatgaacat
     6121 gctcgaatgg tagatgaaag atatgtagta actgaagaag agagaaaaca cattttggat
     6181 acatcatttg aaagtatgaa tcctttaaaa ttaaaagtat tttcttcaaa agaaaagaaa
     6241 aaggtcgtaa ttttatctaa gattttggaa caatttgaac gtgggaagaa atattcagaa
     6301 aaggaagtta atcagatttt agaatctata tatgatgatt ttgcaacaat tagaagatat
     6361 ctaattgagt atggttttat gagtagaaat aaagaatgta caaaatattg gttaacataa
     6421 ttaaaataat aaattttact aatgtgtaat gttattttta tctaggggga ggagtcaaat
     6481 atgaatgatg aattaaaatc tattttatac aataaaggtg tagacattgt tcgctttgtt
     6541 gatatatctg aatttccaat aaatcaaaca caaggttttt ctaaagcaat tttattttgt
     6601 atagggttat caaaaaaatt tattacagat atatacaata acttacctac agatagtgat
     6661 gagtttttgg aaaaagagga gaaagtagaa gaattagctg actggatttc aaaatatatt
     6721 caaaataaag gatatagagc atattctcaa tcggagaaaa acaatctaga acatggatat
     6781 tttgaaaaag cctatattaa tccagaaatg caatcaggga ttagtccttt accacataag
     6841 acaatagcaa atattagtgg cattggattt atgggaaaaa ataatttgtt tgtaacagag
     6901 gaatatggat gtgcttttag catgtgtaca gtacttacag atgctccaat ttctgttgaa
     6961 agatatcctt tgattgattc aaaatgtatg gattgcaatg tttgtgtaga gaactgtcct
     7021 gcaaaagcta tacatggaaa tgaatggact ttacctggaa aaagagaatc tattattgat
     7081 gtttcaaagt gtttttgtgt attgaggtgt atgatgagtt gcccatggtc attaagatat
     7141 gcaaatcaaa aataacttta ctagagaagg tattaaattg aatattataa aatttttaaa
     7201 atgtaagatt atataagatt tctcaaaata aaaatttgta tagaacactc agaaatgagt
     7261 gtttttttat tgggattaaa ttatattgat acaaagctta actattttaa tcattatata
     7321 aaatttgtat taaaaaatta agaataactt ggtaatagtg aaaatataat gagattataa
     7381 ttgatttata atataactat aatgatattg taaaaatgaa aattgtcctc tcaaaattcc
     7441 caaagagagt aaaagtcact tcagttatag tcaaaaaaat ttttctgtga aaagaattta
     7501 gatttgaaaa ggaggaatta atcatggaaa ttgaaactaa aactccagat tggattaatg
     7561 tactagaatt aaacagtaaa atagatatta ctgggaaata tgatgtaagt aatatgatac
     7621 aaaatattgt ctctgataag tcactagaag gttccattat atattttcca aaaggtaatt
     7681 acttatttga gaaaggtatt aaaattagtc aacaaataac acttcaagga gattcttatt
     7741 atggaggagg taatcaagta tcaaatttag ataaaaaaca atcaatcata ggaacaacca
     7801 attttataac ccgaggagtt tctaatatga gtattattac attgtcagga acaagtcaat
     7861 gtatcaaaaa tattaatttt tattatgata gtcatgatat agagaaaata ccgcctaaaa
     7921 atgtatctgc tattacagaa tatggagaaa cacaaggact atctcatttt gaacatctgt
     7981 ttatttctgg attttcaggg ataggaattg aaattccata ctattcaact ggtaatgata
     8041 ttactgttag ttcatgtggt cttggtatga ggcttggaga gaagtcaatg ctttctagta
     8101 gcaagattta cgaatgtaaa aatggaatgg agattactac tggtgttagc ttaaataatg
     8161 tgagaataga agaaataaaa gaaataggta ttaataataa aggatttggt tttaacctga
     8221 ttatgaatct tatggtcgac caatgtggat attgtggatt tatgtttgaa aaaatgtgct
     8281 atagccagat aactgccaga attactagat gcggtcagta ttataaaagt gttgattata
     8341 atgcttatga gaatatggaa gataggaaag aagaagctta ttccattttt tatggagata
     8401 ttttagaaaa ttgcaatatt gtattgatga atagtaatat tgataccttg gaaaatgatt
     8461 cacttgatga acataagata tatgtaatca aggcaaatga aacaaacaat gtaatgctaa
     8521 catgttatgc tgaagcagat aactttatac aatgtgcaaa gggcaacttg ctgttagaaa
     8581 atggtgaaaa tacttataaa ttttataatg gtgagatttg ttctgtagga ggtgttcaaa
     8641 tttctgatat agatgaaaaa gatttaatca aaataataga gaacactttg tatatagatg
     8701 ataaaggtaa gtatttaatg gttcctagat taaaaaagaa tactgtaata tattcagtac
     8761 tagattctac agaagaaata aataaattac atggtggaat atgggaacag attgatgtaa
     8821 aagtattagc tggggaatcc atgtactatt ataaaaagat aaaagattaa attatcaaac
     8881 taggaggtag tgattaatgg aagagtctat aataattaaa gatataagtg gctggattaa
     8941 tgtccttgat tatggaattt gtaatgatga aacaaaagat gtaagtttta agttgcaaga
     9001 gattatggat gctgtaccag aagattctat tatatatttt ccaaaaggaa aatacttatt
     9061 tgaaagtggt gttaagatta agaaacgatt aactttatgt ggagattctt atattttggc
     9121 gaatgttcct aaaaatactg ctggagtaac acaatttact tttaaaggaa agaaaaatcc
     9181 taaaagtgaa aatatagcaa ttataacagc aaaaagtgta agacactgta ttaagagcat
     9241 ttgcttttat tcagatagtt gtgatagaga tatgacatct aatcatgaac ctccaacaga
     9301 tggaaaacct agatttcatc ataatgtaat tataaattat agaaatatat cggctattgt
     9361 atgtgaagat aaacagggag aatcaggtca ttataaaaat ctatctttta gtggtttttc
     9421 tggaattgca cttgatatgc ctaataattc tacagcaaat gatattacaa tttttacatc
     9481 cggacttgga attagtacag gagaaaattc aactattgct aacagtaagg tttggggctg
     9541 tgttgatgga atgataattt ctactggaac ttttataaat ggtatgaggg tagaagaaat
     9601 aaaaaaagtt ggaattaaaa atattggtag aggtttaaat tttattacaa atatcacaat
     9661 tgaccaatgt ggatattgtg gagtttggtt tgattctata tccaatgttc agatatcagg
     9721 aaatatcaca aggtgtgggc agtactatta tgatgtaagc tatgataatt atttaatgtt
     9781 ggataataga gttaaagaag catattcttt attctatggt aatttattgg aatcaagtaa
     9841 tatagtttta tcaaatacca ataaagataa ttgggaagat ttcaaagaag ataagcataa
     9901 ggtgtatctt gtagaagcac ttgaaaccaa gaatgtacta ttaacatgca atatagatgc
     9961 tactgactgg attgttaagt cagaaaaagg aaatttaacg tacaataata tgcgacaaac
    10021 tgttatattt tacgatggaa aactaagtag tatcaatgga attggtatat ctgatgaaga
    10081 ttatggggat aaagtcaaga taaaaaaagg aaatatatat ataaatagta aggataagga
    10141 tattaagatg cataaaccag aggtaggtga cattatgtca acaattatta aagataaaag
    10201 tattctttct gctcattatg ggggaacatg ggagaaaatt ggtgaaaaaa tagagtttgg
    10261 aataactgta cactattata aattgataga gaaataagta aatacgtagt atttgaatag
    10321 atgtatttta ttggagagct aggactatag ttttaatgta aatggtttgg taatagcatg
    10381 gattgaataa tataaaaata atataaaaaa ttaatttgat tatgtaaaat tctatgaagt
    10441 agtaaaactg tgactttagt cataggaggt tcaaactaag taaaagggaa aggaatatat
    10501 aatattatgg aaggaagaac tgggattaaa gatggaagat ggattaatgt agtagaaaga
    10561 ggtattaaaa atgatggttc gtacaacgta ggtcctgctt tgcaggatat tattgatgaa
    10621 atagaaacag atttttgtgt cttatatttt ccaaaaggta aatacctatt tacaagtggt
    10681 gttaaaatta ataaagaaat tgtattgcaa ggtgattcta attcatctac aggcactaca
    10741 caatttataa ctcgtggagt agaaaatatg agtattatta cattgacagg gaaaaaacaa
    10801 tgtattaaaa atataaattt ttattctgat agttgtgaga tacaggtaaa taaagaacca
    10861 ccgacaaaag gtaatcctaa atatcattat gagatgatta ttaataagaa tgaaaagaat
    10921 gttgagatga ataatgtatc agcaatcctg tatgataaca gtaagaatat gattaaaggt
    10981 ctaggtcatt atgaaaatat ttatataagt ggtttttcgg gtacaggaat taggatacca
    11041 tattactcaa ttgtaaatga tattacagtt tcatcttgtg ggctgggtat tgatacagga
    11101 atagacacaa ttatttctaa tagtagaata tctaagtgtc aaaatggaat gaggattaga
    11161 actggaacta gtataaataa tgtaaggatt gaaaaaatac agaaagttgg tcttataagt
    11221 agtcaagtta aggggtatga ggggtatgga agttataaaa ttaataatat tacgattgac
    11281 caatgtgggt attgtggctt ttcatttgat tcaatgaaag atagttattt ttctggtatt
    11341 ataagaaggt gtggtcagta ttattataat acagattatg acacatattt aagtattaaa
    11401 gatagagtag aagaagcata ttctttattt tatggaaatt attttacaga atgtaatatt
    11461 gatttagtta gtgataataa ggataactgg gatgatggct tagaatataa acataaagtt
    11521 tatgtattta aaacccttca aataccaaaa ttaacattga gatgcaatat agatgcctat
    11581 gattatatcg ttaaatcggt acatggagga ggtaatttaa tgttaaaaaa taattttaac
    11641 aattatatat ttattgattc atctaatcca gaagttatta atggaattaa ttttaattat
    11701 caaaagaatg gaaacttgtt aaggttaaat aatggtaaat taagttataa aaatggaaag
    11761 gaagaaacag ctatattcag acctaattat ggtcttattg tagcatcaac agattcagaa
    11821 tgtgaacaaa tagagactag ctatggagga aaatgggaga agcttggttc tgaagtaatt
    11881 ggagaagaaa cggtatatta ttataaacat catgaaccag ataaaacaaa ccaaatacaa
    11941 taaaatttgt attatataat gatataaagt tttaatatat agtgtttacc tttaatgtaa
    12001 tattggtata tttgtatagt attaaatttg ttaaaaagta aaattgtagg gtgcttgaaa
    12061 atagcacctt ttttagtttc attataaaat tagttttgaa agtttaggaa taatgtatat
    12121 ttatttttaa agaattataa ccttattaag gatattttaa atgctagaaa tgaatcagta
    12181 aatcatactg gaattatctt aaatgttttg attaaattaa gtatttagag ctataattat
    12241 attaaaaata tattagaaca taattatatt actatggagg ttaaatatga tagtaagtgt
    12301 gagtagaaga actgatatac ctgctttcta tagtaagtgg ttttttaaca gacttaaaga
    12361 aggttttgtt tatgtaatta atccaatgaa tccaaaacag gttagtaaga tagagttaaa
    12421 tccacatact gttgattgtt ttgtattttg gacaaaggat gtaactccaa tgttatatga
    12481 tttaaataaa ttaaaagact ataaatatta ttttcactat actataacat catatggaaa
    12541 agaagtagag acatgtattt tagataaaag aaaggtaata gattcattta aggaattgtc
    12601 tagaaaaata ggcaaagaaa gagtaatttt aagatacgac ccgattttct taagtgaaaa
    12661 gtacacagta gattatcata tcaaagcatt tgcgagttta tgtaatcagc ttgatggatt
    12721 tacagaaaaa tgtataataa gttttatcga tttatataag aaaacaaaat ttaatactaa
    12781 agcattacat ataaagccaa tagaaattac agaaatagaa acattatcaa aagagatttc
    12841 taagattgct cataagcatg ggataacact tgaaatgtgt tctgaagaat ataatttaag
    12901 taaatttggg attggaaaaa gtaagtgtat tgatgataga ttaatatcaa aaattatagg
    12961 atgtgaagta gaggtaaaaa aagatagtac acaaagagat atttgtggat gtgttaaaag
    13021 tgtcgatata ggacaataca atacttgcag acattattgt ctgtattgtt atgctaactt
    13081 taattatgca aaagtagaag aaaactgtag gctttataaa gaagataata aattactcat
    13141 agatacttta agagatgatg caaaaataag cattagagat atgaagacta ttaaagtaga
    13201 taagaataaa caaattagta tatttgatta aaataaatat actaattacc ttaatatatt
    13261 aagaatatag atacttttga taacaaaatt aaacttaata gatattttct atataagaaa
    13321 gatggtcata atatgtcaat ttgaccatct ttttgatata attaaaacac aaataattta
    13381 gtaacaaagg aggcagaact tgaatttatt aaaaaaatat cctataccta ttgcaagttt
    13441 gattttgtca atctttactt taggtaactt attacaatct tatagtgaat cattacgtaa
    13501 tgtaattgga tttataggat ttattttgta tgcaatttat atagtcaaaa taatattact
    13561 tagaaagggt gttaaagagc aattagagaa cactttaata tcaagtacat ttccaactat
    13621 aacaatggca acaatgatat ttgctacata tataaaacca ttatctttag aattaagtat
    13681 tatgatttgg agcataggaa ttgtgggtca tcttgtgtta atagtcttat ttagtaagaa
    13741 gttcttagtt aaattctcaa taaaaacggt atttccatct tggtatatag tatatgttgg
    13801 gatagttgca gctagtgtta cggcaccagc atttaatcaa cttttgattg gcaaaattgc
    13861 tttttggata ggatttgtat cttatatagt tcttatacca gtaattttaa agagagtatg
    13921 gataatcaag gaaatgcctg aaccatcttt accaagtata gctatattag ctgcaccagg
    13981 gtcactatta ttgtctggat atattaattc tttttcagat aaaaataatg tagtattata
    14041 cttgttattc atattatcaa ttgtatttta tattatagtt ttgggatatt taccgaaatt
    14101 attaagattg cctttttatc cgagttttgc tgcatttaca tttcctatgg caataagtgc
    14161 aatgggtatg aaactaatga atggttattt aactaaatca ggtcatttta taagttggat
    14221 ttcatatgct tctaagttag aagagataat tgctacagtg attattttat atgttttagt
    14281 tttgtttatt atatatctta gtaaatctga aactacatct aaagctaaat aagaattatt
    14341 aaatatgtgt atttaatagg aatattgata taaatatatg aagataagat aaaccataga
    14401 aacttaattt agcttaattt tattggagga aaacaaatga aaacaaaaga agaatattta
    14461 aaagaagtta gaaacatatc agaagaatac tttagaaaag gagagttttt ttgctctgaa
    14521 gcagttctcc agacaataaa tgatgcttta ggccaacctt taagtccaga gataactaag
    14581 ttagcatcag gatttccgat aggacttgga aaggcacaat gtctttgtgg agcaatatct
    14641 gggggagaga tggctttagg tatagtatat ggaagagttc atggtgaagc tatgaatcca
    14701 aagatgtttg agcatgctaa aggtttgcat gattatatta aaaaagagta tggtgcaaca
    14761 tgttgtagag ctattacaaa gaaatgggat ggtgataact ttatgtctcc cgaaagaaaa
    14821 gagcactgta ttaaaataac aggtcaagtt acagagtggg ttgcagataa gcttatagaa
    14881 gatgaaaaac ttaatatcaa atagtgtaat attagttggt gaatttataa agttaaattt
    14941 acttatagta taagatactt gatattctcg tattgataga aaaatttata gcatatgtat
    15001 ttaatgaaat atacttatat ttagagtgtg gtttaatttc ataaatacat atgctattat
    15061 tttaagtata taagcaaatt tatttaaata aatagaaaca gttaatttat atgtacaaaa
    15121 ctatatatga ttagtgtaaa taaataccta ttttataata ctcaacataa agacaccaga
    15181 catgattagt attataccaa taaaaatagc aagccattct tttttgttct tcttttcttt
    15241 aaaaaatata atccctccta aagtggctac tatcacacaa ctctgagaga tagaaaaact
    15301 tattgtaact cccaatgtat tagtcgttat gaacatacct aaatttgcta ttgaccacaa
    15361 tataccagta attaaattga ataaaacatt ttccctatat acaatctctt tagaaaaata
    15421 cttgacaata atagatgaag ttatcaacat accaacagat tgaggtaata taattctata
    15481 tccttcaatt aaaaatattt gattagttac aacatataat gataaaaata gagatgataa
    15541 taatacaact ccaacacatt ttaacatatt tttatctgta gattttttag tttgataatt
    15601 tgtgagaagt ataccaagta ttatacataa tattgataaa gaaccaatca gtaacatttt
    15661 agaactggtc cattcattaa acaagattac agctaataat gtcgtaaaag ttaattgtga
    15721 accatttgaa attggcatta ctttagatac actagcaatt tgaattgctt taaactgcat
    15781 taattgtccc aaactccaga atatgccaga aatcatactt atacaaaatg aaaaaaatgt
    15841 aatttcagga tgtataacca gagttattat agtggaaaat ataaacgcac ttatggatgt
    15901 acctaaaagc tgttcatgag gagaactttt ctttagattt gcaactatgg gcatcaatcc
    15961 ccatcccatt acaggaagta aagcaataat tattgtcagc attaaactat ttcctttcat
    16021 attaaattta gacagctttt aatttatcat ctaaaattaa agtgttcaat acataaaaat
    16081 taccgtattt aatcaggaaa tattagtagt tttttagaaa aacattttcc attaaaataa
    16141 agacaaaggg aaggagtaaa attgaaaatg ttaactgata tgattcaaaa tcgtgaaatt
    16201 aaaataataa agtttttatt aaaagaaggt catacaactg taaaagcaat agcagatgat
    16261 ttagatgttt cagaaaaaac agtttcaaaa tctcttaaag aagtagaagc agtgcttaaa
    16321 aagataggtc tttctttaat aagaaaacct aagataggtg tttatattga tggagattta
    16381 aaaaaaatac ttccttacct agataataaa ggaagtcaaa ttcctacaac cagagaggag
    16441 agagttatct atatatattc agtactttta aagagtaatg attatattac tatacaacaa
    16501 ctttctgatg aactatatat aagtcgtgga actattgaaa gggatttagc agaagttgaa
    16561 aaactgctaa aaaacgaagg gatttccata tataagaaac ctagtaaggg aataaagtta
    16621 aatataagtg aaagagataa aagacaactt actgctaagt ttatacataa attttggagt
    16681 agaaactggt atattaagca agagcaagaa agcttttatc aagcatttga aaagatacaa
    16741 gcagatgtaa atggtatttt ttcggaagat gatttatcaa taattattga tattcttagg
    16801 aatttcagca gagaaagaaa atttgagttc tctgattatg aatttcagtc attagttatt
    16861 catttggcaa tagctgtaga gagaattaga aaaggcgaat atatagaaga aagtttgtat
    16921 actggtaata tagaaaataa tcaaatgtca aataccaatt atttagcaaa acaattagaa
    16981 gaaaaatttt caatattatt accaaaatct gaaattggat atataaattt acatttagta
    17041 gcgtctgacc aacatagaga tactaatgaa ctaaaaaata cttcaactaa tccttgtgag
    17101 acaagtacaa ataatatatc aaatcttcgg gaaattataa aaaatacatt gtatgagaca
    17161 gcttatgaca aggatttaat tgatggattg acttcacatg tacattcatc aataaataga
    17221 ctgaaatatg gacttagtat taaaaatcca tatgtaaata caatcaaaca aaacttttct
    17281 ttatcatttg agcaagcttt agatttaaag aaagtaattg aatttatata tgatattagg
    17341 ataaatgatg atgaatgtgc ttatatagca ttacattttg aagcttttag agaaagaatg
    17401 aaagaacttc cacaaaatat tagagtaatt ctagtatgca gtacaggatt aggttcatct
    17461 aggttattgt ctgcaagaat aaaaaaatac tttccaatga taaagattga aaaagtactt
    17521 tccgtacaag aattagtatc cagtaaaatt gatgcagatt tagtaataag tactatttat
    17581 ttagagttag aaacaattcc aaccatagta gttagtccta ttttaaatga aagtgatata
    17641 agaaccttgg aaagaaatat acaaaatgtt tcaagttgta cttctaagaa gtataaatct
    17701 ttcagaaact taatctttga agaaaatata ttgttgaata ttgaagcttc aaactatgaa
    17761 gatgcaatta agtatataac agataaccta ataaaaaaag gttttgcaaa agaaggtata
    17821 gctaaaagtg caattgagag agaaaattta tcttacactt ctttccaatc aatagctaca
    17881 cctcatgcta atcctagtta tattaaaaaa tctaatatat ctgttgcaac gttaaaaaat
    17941 cctattacat ggggaaatga aaaggtggaa gttattttct ttatttcatt agaaaatgac
    18001 aaaaacctag agcttgatga aatatatgaa gttttttatg actttattga caataaaaaa
    18061 aatataaatg acatattaag tgcaacttcc acaaaggaag tgtacgattt gctagtggag
    18121 gagagtatat agtgaaaata tcagatgtat tgaaaaaaga gcaaattgtt tttaatcttg
    18181 atacaaatac aaaggaagaa acaataaaaa aattaagtag tatatttgtt gaaaatggga
    18241 ttgtggacaa tgaagaagaa tatgttaagt ctgttttgga aagagaagaa cattcaacta
    18301 caggtattgg taatggtatt gctattcctc atgggaaaag taatgctgtc aaaaaatctt
    18361 ctataatatt tgcaaagact aagcataaga ttgaatggga tgctttagat gaaaaaccag
    18421 tagatagtat tttcttattg gctataagca atattgataa ggatagcaat catcttgtac
    18481 tattatctaa attagcaact aaacttatgg atgatgacaa tgtagatgca ctaaaattag
    18541 ccaattcaga acaagaaata atcaatatat ttagtgaaga aggagagatg tagaaatgaa
    18601 aagaaaaatt atagcagtta cagcatgtgc aacaggagtt gcacatacat atatggcagc
    18661 tcaagcatta aagaaagctt caaaaaagat gggtcacctt ataaaagttg aaacacaagg
    18721 agctacagga atcgataatg agttaactac aaaggatgca caaattgcag aagtagtaat
    18781 atttgcagta gatactaaag tcagaaatgc tgaacgtttt gagggaaaag aaatactaga
    18841 agtaccagtt aatgcaccta taaaggatgc agagaaagta ataaatgatg cattaaagtt
    18901 aattgacact aaataaatgg agggaaatgt aatgttgaaa aagatagtct ctgagttaaa
    18961 aaagcatgtc ttaactggaa tctcatatat gattccatta gtaattgcag gtgctatgat
    19021 aatggcgata tctcgtgtag gtggttcttt ctataatata ccagatatat gggatgctaa
    19081 atatgcagaa agtgctagta gtattgttag attattacat gatttagatg ggtttggagg
    19141 tactgcatta ggattaatgt ttcctgtaat tgctgcattt ataggatttt ctatagctga
    19201 taaattagct attgtaccag gtcttgttgg aggtatgatt gctaaagata taggtgcagg
    19261 atttttagga gctttggcag ttggtcttat agcaggatat acatgtttgt ttattaaaaa
    19321 tcatgttaaa ttaccaaaat cagcagcttc aatagtacca atatttatag taccagtatt
    19381 tgggacatta atcactgttg tattaatcaa ttatgtgata ggtatacctt ttgccacatt
    19441 aaatactgga ttagaaaatt ggttaaatgg attatctggt acaaatcaga tactaatggc
    19501 agctattata ggggctatga tggcagttga cttaggtgga ccagttaata aagcagcttt
    19561 aacaacatca cttgcattat taacaagtgg aatttatgcg cctaacactg ctgcaatggt
    19621 aggaatagtt ataccaccac taggattggg acttgctact atactagcaa aacaaaaata
    19681 taataaacag ttacgtgaag ctggaaaatc atctcttata atgggtttaa ttggagtaag
    19741 tgaaggagca attccttttg cggtagaatc tccattaaaa gtaatacctt catgtgtaat
    19801 tggaacagca attgcttcag ctatggcagt tggtttaggt tcagtaaatg caactccaat
    19861 tagtggattt tatggatggt ttacagtaga gaattggcca atgtatgtgt tatcaatagc
    19921 agttggaaca gtaattgtag ctggattagt tatagtattg cgtggaaatc cagtgattga
    19981 ggatgatgaa tttgaagatg atgattttga tgaagcagag tgggaatctt aaaatttaat
    20041 aactatttaa gacgtgttat aagctatatt caatatgaat cttatactaa ataaagtgtt
    20101 aaatattcag tttagactta aatataaaag ataaagaaca gaggagaata aatgataaaa
    20161 gcacatattg ttaatcatac acactgggat agagaatggt attttacttc tggagatgca
    20221 ttagtactta gtgaacaact atttacagat gtaattgatg aattagaacg taatccagat
    20281 gtaagttttg tacttgatgg acaactttct attttggatg attatgtaca attacacaaa
    20341 gaaaaaatag aaatcattaa aaagttaata aaagaagata gactacatat tggaccatgg
    20401 tttactcaaa ctgatgcatt ctttgctaga ggagagtcta ttttaagaaa cttaatgatt
    20461 ggaatttttg aaagtaaaaa gtatggtaag tatatgaaaa ttgggtattt gccagataca
    20521 tttggattta atgcacagat gcctatacta ttaaagcatg taggtcttga taatatattc
    20581 ttttggagag gtatacatct tggtaaacag gttcaatcac catattttaa gtggaaaagc
    20641 ttaaatggag aaagttatat atatgcagta aatatgccac atggatatgg aactggtatg
    20701 cttttagagc cttcattaaa gtatgttaat ggacgcttag acccagctat tgattttatt
    20761 aaatcatata ctgatgtaga tgaggtgtta ataccttctg ggaatgacca gttaaatata
    20821 ataggtgatt tcaaaaataa aattaaagcg attaatgata tcggaaagta ttcatatata
    20881 acgagttcat atcaagagtt tttaaaatat ataaagtcca tagaatcact agaaagctat
    20941 agaggtgaat ttagagaacc tgtattagca agaatacata aatcaattgg ttctagtaga
    21001 atggatataa aattagcatg tgataaacta gaaaacaagc tgattaaaag aatagagcca
    21061 ttattagtaa ttgctaaaaa gagcaaaatt gaaataagca atcagttact tataaataca
    21121 tggaaaaaac ttttggaagg acaagcacac gatagtttag ctggatgtgt aactgatact
    21181 gtaacagatg atattcttca ccgtataaga gaagcaaatg aaatatgtga cagtattgaa
    21241 aatactattg taaaaaagat ttcagaaggg cttaaattat ctaaaaatga tattttagta
    21301 tttaatacag aagctaagcg ttttaatgga tataaagaga tacaagttgt aagtgatagt
    21361 aaaaatattt attttaaaga taatttagat gctactataa ttgaagaaac ttatgtaaaa
    21421 ccgagagaaa atgtattaga agaaacacct gctggtaata tatttattga agaaccagga
    21481 tattatatat taaaagtaag aataaatgta cagcttcctg cacttggtta taaagttgtt
    21541 agttttgaat taagtgataa agaaatggtg tcacttgata aatctaatga cacttttata
    21601 tctaatgata attataagat aatatatgaa gatggaagct taaatttaca acttaaagat
    21661 ggaacttaca tatcaaactt tttaacatta aaagatagtg gtaatgcagg tgatacctat
    21721 gacttttctc cactaaaaaa tgatgagaat ataaaacttt cgtttgataa agtgtataca
    21781 gaaaaagctt taggatatga aaaaatggtg gttaaaggta ctactttact accacttaca
    21841 ttagaagata gaaaaaacaa atatcttaat ggtaaattaa atgtaaaagt aagtattgtc
    21901 ttatctaaag aaaacccatt gatggatgta aaactttgtg ttgataacac gatatataat
    21961 catagactac gtgttcatat aaaaactgat ataaaagata ataaaaacat agcatcattg
    22021 ccatttggat atataacaag aaaaaatgga gttttagaca actgggaaga tatttacagt
    22081 gaaatgccta tagatttaga acctttggaa agtaatatta cgcttacaaa ccaaaataga
    22141 agttgtactg tatttactag aggaataaaa gagtatcaac atattgaaga tgaaattgca
    22201 ttgaccttac ttgctactac agatgaacta ggaaagcctg atttattata tagaccagga
    22261 cgtgcttctg gtgatacaac taaaaaaggt catataagaa ttaaaacaga taaagctcaa
    22321 atgcttaagg aattagaatt ttcatttgca atatatatgg attctaaatc atttgatgaa
    22381 ttggaaattg cgaatttaac ccacaattat ctaagggaat gtgtaaatta tcaattacag
    22441 gattacaatt tctttttata tcatatagat aataagattc aaaaatcaat tgtcaataaa
    22501 actgtaagta gagaattaga aataatatct ttaccagaaa actatcttat atcagcatgt
    22561 tatccttctt attacagtaa agataaattt ataataagag tggaaaatcc aagtagtaat
    22621 agaatgatat tggatgagca aatatttaaa aatagaaatg ggaaaattgt aaatgcaata
    22681 gaagatgtag aagaggaaca ggtatatgaa atagcacctt ttgacgttat ttcaatatta
    22741 ttggatttat aagtgtaaaa gtaagtttat tgtttaaagt ctgacagata ttaatctgcc
    22801 agacttttta atttgtataa atttgggtaa atattaatga atggttttag tatttaagta
    22861 tgaatttagt atttaatata gaatttattg aagtagaata attagtttaa caggataagc
    22921 agtttaaata aagaattatt atattagagt gattttatat ataatttaaa ataataaaat
    22981 tttaataaaa tgagttaaag attgtatatt aatagagttg atgtagttac aaaaaagtta
    23041 ccaattgtta tgtatttaca aaactgatat attaattaca taaa
//
LOCUS       DBGJFU010000058        22586 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.058, whole genome shotgun sequence.
ACCESSION   DBGJFU010000058 DBGJFU010000000
VERSION     DBGJFU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.058"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            1018..2688
                     /gene="pdcB"
                     /locus_tag="ACOIAS_002091"
     CDS             1018..2688
                     /gene="pdcB"
                     /locus_tag="ACOIAS_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase PdcB"
                     /protein_id="HGZ2701802.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHKMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESSKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2810..3544)
                     /gene="pflA"
                     /locus_tag="ACOIAS_002092"
     CDS             complement(2810..3544)
                     /gene="pflA"
                     /locus_tag="ACOIAS_002092"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HGZ2701803.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3704..5935)
                     /gene="pflB"
                     /locus_tag="ACOIAS_002093"
     CDS             complement(3704..5935)
                     /gene="pflB"
                     /locus_tag="ACOIAS_002093"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGZ2701804.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            6294..7289
                     /locus_tag="ACOIAS_002094"
     CDS             6294..7289
                     /locus_tag="ACOIAS_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGZ2701805.1"
                     /translation="MFITVVLFLVGFLLITKGADIFINCTVEIGKKTNISEIILGATI
                     VSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTGLALGLVVFISPFNVDKKMFF
                     SKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMVNNYRSVVGKSDTKNRNIKN
                     NTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGSQILIESGVIIASFLNIPQ
                     GIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANILNIVSVIAVSAIPNNIP
                     ILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGILMLFTYFLYISILYFMYII"
     gene            7380..8993
                     /locus_tag="ACOIAS_002095"
     CDS             7380..8993
                     /locus_tag="ACOIAS_002095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGZ2701806.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9268..11253
                     /locus_tag="ACOIAS_002096"
     CDS             9268..11253
                     /locus_tag="ACOIAS_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2701807.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            11258..11692
                     /locus_tag="ACOIAS_002097"
     CDS             11258..11692
                     /locus_tag="ACOIAS_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGZ2701808.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11721..12281
                     /locus_tag="ACOIAS_002098"
     CDS             11721..12281
                     /locus_tag="ACOIAS_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGZ2701809.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12345..12845
                     /locus_tag="ACOIAS_002099"
                     /pseudo
     CDS             12345..12845
                     /locus_tag="ACOIAS_002099"
                     /inference="COORDINATES: protein motif:HMM:NF015569.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
     gene            12885..13304
                     /locus_tag="ACOIAS_002100"
     CDS             12885..13304
                     /locus_tag="ACOIAS_002100"
                     /inference="COORDINATES: protein motif:HMM:NF019286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HGZ2701810.1"
                     /translation="MAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFP
                     LLSPFLGPGAVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAE
                     AEPDTVQVGVPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13345..13710
                     /locus_tag="ACOIAS_002101"
     CDS             13345..13710
                     /locus_tag="ACOIAS_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGZ2701811.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13811..14584
                     /gene="srlD"
                     /locus_tag="ACOIAS_002102"
     CDS             13811..14584
                     /gene="srlD"
                     /locus_tag="ACOIAS_002102"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGZ2701812.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14933..17371
                     /locus_tag="ACOIAS_002103"
     CDS             14933..17371
                     /locus_tag="ACOIAS_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HGZ2701813.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMALHKVNYVSDELDTTSNKVNYVSDELDMALHKVNGVSDELDMA
                     LHKVNCVSDELDMTSNKVNYVSDELDMTSNKVNYVLDELDITSNKTDYISNELYNLSN
                     KSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTVTLCDMFIP
                     CTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKR
                     TYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLG
                     ISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINQRMAKKVG
                     IEEKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQ
                     VTYNESMVEIIRQN"
     gene            17459..17794
                     /gene="spoIIAA"
                     /locus_tag="ACOIAS_002104"
     CDS             17459..17794
                     /gene="spoIIAA"
                     /locus_tag="ACOIAS_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HGZ2701814.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17808..18236
                     /gene="spoIIAB"
                     /locus_tag="ACOIAS_002105"
     CDS             17808..18236
                     /gene="spoIIAB"
                     /locus_tag="ACOIAS_002105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HGZ2701815.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            18259..19023
                     /gene="sigF"
                     /locus_tag="ACOIAS_002106"
     CDS             18259..19023
                     /gene="sigF"
                     /locus_tag="ACOIAS_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HGZ2701816.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            19303..19740
                     /gene="spoVAC"
                     /locus_tag="ACOIAS_002107"
     CDS             19303..19740
                     /gene="spoVAC"
                     /locus_tag="ACOIAS_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HGZ2701817.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19907..20920
                     /gene="spoVAD"
                     /locus_tag="ACOIAS_002108"
     CDS             19907..20920
                     /gene="spoVAD"
                     /locus_tag="ACOIAS_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HGZ2701818.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20934..21296
                     /gene="spoVAE"
                     /locus_tag="ACOIAS_002109"
     CDS             20934..21296
                     /gene="spoVAE"
                     /locus_tag="ACOIAS_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HGZ2701819.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            21479..22495
                     /locus_tag="ACOIAS_002110"
     CDS             21479..22495
                     /locus_tag="ACOIAS_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2701820.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         8839 a         2532 c         3899 g         7316 t
ORIGIN      
        1 ttttataata aaatagctat aaaaatatac atatcaaatc aaattaagaa gtatttcatt
       61 tctaagaaat atcctaacat aaaaacaaaa aatgttcata aatttaacta ttaacataga
      121 taatttttag aaaaatatct aaattttcaa taaatagtaa cttttttata acaaatggaa
      181 actatggatg taataattaa aataaaaaat attattttat atttataaac attgacattc
      241 taattttgta gtgtaatcgt attttttatt aattcttaca aaatcaatga aaataagtat
      301 atccatttta aagtacataa atttaaaaat ccagaaaaat atacttaaat tatgtaagta
      361 aaagaggggt tgaatcagtt gaaaatgtaa tattagcaaa tagtacatca atcctatata
      421 ccctgtaagc aactctattt tcatgtacta aaaatgtacc tattttatta actgaaaaga
      481 ataaattaaa tgtaagtaca taagaagata gaaaaaatta aaaacaaaaa atatttatat
      541 aaataaatat aaaaaatgat ataatcccta ttaaaaaatt tatagatgat gaagaaatat
      601 caaaatctta tattatagga agagacactt ctttgccaaa atctatagaa aaacaatacc
      661 aaatccaaag agaataggcg gagaaaaaaa taaaataaat tcaagagtaa tagaaaagtt
      721 ttatagaata aagaactgat aactctttat ataacgaaaa atagaatttt aagagaaaat
      781 gatttaataa ctgatttaac atggtgtttt agcaggtaaa aataaatcac cagtaatgat
      841 tatagaaaaa ttactttatg aatctcaaaa aacttagtta agagtaagag atttgataag
      901 ataactaaag ttggggaaat gtgtttgatg agacaaaaaa ttagtaaaat agtataatct
      961 aaataaggat attttgttga ttaaactaca ctaatagtaa tattgtaggg ggaaaagatg
     1021 agagaagaat cgaactcttt aagtttagac catatagaaa ttttctttga acttttatct
     1081 caaagtacag aagattatat atttttttgg gatataaata ggaataaatt taaaatttct
     1141 tcagcaattt ttgatgaatt taacttaagt aaagagatag aatctgattt ggtcaattgt
     1201 tggagtaaaa ttgtttatcc agatgatgtt caaatatgga aagatgatat acaagaatta
     1261 ctacatggaa aaaaaggtga gcacaattta gaatataggt tgattaataa atataaagaa
     1321 atagtatgga tttcttgtag aggtaaagtt tatgtgtcgg atgacccaaa aactatattt
     1381 ctagtcggga gaataaagaa tattggtgaa aaaaataaat ttgattctat cacaggaacg
     1441 tggaatagag aacaatttga acataaaatg aattatctta taaaagagaa aatttataaa
     1501 aatggtgcta tgtttattat ggatattgat aattttaaaa atataaatga aaaatatgga
     1561 cattcttatg gtgacaaagt gttgcgtgca attgctactg aagtactgga atatcttcca
     1621 aaagatgtaa gattgtatag gttagatgga gatgaatttg cattttttta ccctatgtgt
     1681 actaaagaaa ctatagagaa aatatatgaa aagatacaga tgtatacaaa tactcagcat
     1741 gaaattgaat caagtaagta ctattgcaca gtgactgcag gcgttgctat gtatccagaa
     1801 gatggagata attatttaga tttatttaaa catgctgata ttgcattgga tatagcaaaa
     1861 attagtggta aaaatagaat taagtttttt tcacaagagc tctatgaaaa taaactaaag
     1921 gtaatttcaa tgcaacaaaa actaagagag tgcgttgaaa ataattttaa tgactttgag
     1981 ctgttttttc aaccccaggt aaatgctgtt acaaaagagg taataggggc agaggtactt
     2041 ttaagatggc attcgagtac ttatggagaa gtatctcctg ttgaatttat accaatacta
     2101 gaacagagta atttaattat accagttggg aagtggatta taaaagaagc tgtaaaacag
     2161 tgtaaagaat ggcataaaat aaatccagac tttaaaatat ctgtaaatgt atcgtatatt
     2221 cagttaaagg aagatttttt tagagatttt atagtagaat gtttagtaga gtatcaatta
     2281 agacctgaat ttttaatttt ggaattaaca gagaattgtt ggataccaga tataaattta
     2341 ctaaatgata aatttattag tttaaagggt ataggagtat atattgcaat agatgacttt
     2401 ggtacaggat attcatcttt aaattacctt aaagaactgt ctgttaatat aataaaaatt
     2461 gaaagaagtt ttgttaagaa tattacatat aatagttatg aatatacttt tcttgaatat
     2521 attattaaat tagctcatat tataaattta aaagtttgtg ttgagggaat agagtcttat
     2581 gaagagtatg atatagtaaa aagcttaggg gtagatatta ttcagggttt tttatttgga
     2641 agacctgtaa gtgcctctga attttataaa tttcagctat caaagtgatg tatataaaat
     2701 gagatatata gtatataaaa atagggcaaa gtggaagttt tgaagtttat taaaaattta
     2761 cactttaccc tattttgtta tgtcttctat aaaggattaa tatactacac taactattat
     2821 atactttcac tccaaattta gagattattt cactagcttt ttggacatct tccttagtgg
     2881 catcaggaac atcttttagt tcataatcga tacctaaact ttcccatttg tgtattccca
     2941 tagaatggta tggtagcaat tcaaatcttt ctacattttt caatgtagct acaaattgac
     3001 ctagtttttc taaattatct atatcatcag taatgccagg aactaaaaca tgtcttatcc
     3061 atacaggaat atttcttgat tctaagtgtc tagcaagttt taatgcctta tccataccaa
     3121 caccagtcaa atcaatagat ttttcctcaa ccatatgttt taaatcaagt aaaaccaaat
     3181 cagtattatc taaaactggg tctatagttt ctatatctac aaatccagat gtatccaagc
     3241 aagtgtgtat atcattttct tttgcttttt taaataattc acttaaaaat tctggttgaa
     3301 gagtggattc accaccagag gcagttatac ctccaccaga aaacttcata aaagaagtat
     3361 acttaagagc atccgttatt atttcatcaa ctgtgtattc ttttcctgat ttaatatccc
     3421 atgtatccct gttatgacag tatttgcatc taagaggaca cccctgaaaa aataatatat
     3481 atcttattcc tggtccatct acagtaccaa acgtttctat agaatgtatc tttcccttaa
     3541 tcatagagtt cacctcacaa tatattttaa taaccttatc aatagttata atatttctct
     3601 aaaaaaggcc atcttatacc agaaataatt caaatataag atagcctttt atttttattt
     3661 tttacacagt agtaatctta gttaaagtat aaaaatttaa agactaagcc attttaccgt
     3721 ggaatgttct gtttataaca tctaattgtt gctctttagt taattttatg aagttaactg
     3781 catatccaga tactcttata gttaattgtg gatattcttc tggatgttcc atagcatctt
     3841 ctaaagtagc tctatcaaat acatttacat ttaagtgatg agcattttga gcgaagtatc
     3901 catccatcat tgcagaaaga ttgtttattc tttcagtcat atcttttcct aaagcagcag
     3961 gtactataga gaaagtatta gatataccat cttgagcatg ttcatatggt aatttagcaa
     4021 ctgaagataa tgaagctaaa gcaccattag tatctctacc atgcattggg ttagcaccag
     4081 gagcaaatgg agcaccagct cttctaccat ctggtgtatt accagttttc ttaccataaa
     4141 caacatttga agttattgta agtatagatt gagtaggata agaatttcta taagttttat
     4201 tctttcttat tttattcata aagctttcaa ctaactctac agctatacta tcaactctat
     4261 catcattatt tccgtatttt ggataatctc cttctatttc aaaatcaaca gctattcctt
     4321 gttcatttct aatagtttta accttagcat gttttatagc agataatgag tctgcacaaa
     4381 ctgataatcc agccatacca caagccatag ttctaaatat atctctatca tgtaatgcca
     4441 tttctaatgc ttcataagaa tatttatcat gcatataatg tattacattt aaagtattta
     4501 catataatgt tgctaaccaa tcagtgaatg gttcaaattt actcataact tcatcataat
     4561 ctaagtactc agaagttata ggttcaaatc ttggtccaac ttgaacacca gatttctcat
     4621 ctacaccacc atttatagcg tataataaag ttttagctaa gttaactcta gctccaaaga
     4681 actgcatttg tttaccaatt ctcattgcag atacacaaca agctatacca tagtcatctc
     4741 cccagtatgc tctcattaaa tcatcatttt catactgtac tgaacttgta tctatagata
     4801 ctttagaaca gaaatctttg aatccttgag gtaatttagt tgaccataga actgttaagt
     4861 ttggttctgg agaaggccct aatgtatata gagtatttaa cattctaaat gagtttttag
     4921 taactaatgt tctaccatct attcccatac ctcctataca ctcagttacc caagttgggt
     4981 ctccagagaa taagttattg taatcaggag ttcttaggaa cttaaccatt cttaatttca
     5041 taacaaaatg gtccatatac tcttgtaatt cttcttctgt tacgattcca gcttttaaat
     5101 ctctttcaaa gtatatatct aagaaagtag atgttctacc taaagacatt gcagcaccat
     5161 tttggtcttt aacagcacct aaataaccaa agtataacca ttgtacagct tcttttgagt
     5221 tagttgctgg ttttgatata tcaaatccat aagattcagc cattttttta agttcattta
     5281 aagctgatat ttgctcagtt atttcttctc ttaatctgat aacttcctca tccatgcaag
     5341 aaacttctaa tgatttcttt tgctcatttt tatcttctat taaagcatca acaccatata
     5401 gagcaactct tctgtagtca ccaattattc tacctctacc ataagcatct ggaagacctg
     5461 ttattatacc agattttcta gcagctctca tttctggagt ataaacatca aaaactcctt
     5521 ggttgtgtgt ttttctgtat ttagtaaata tatcttttat ttctgggtct aattcatatc
     5581 caaaagcttc gcatgaattt tcaaccatct ttataccacc atatggcatg acagctcttt
     5641 ttaaaggggc atccgtttgt aacccaacta ttgtttctaa ttctttatct aaatatcctg
     5701 gagcatatgc agctattcca gaaacagttt ttgtatcaac atctaaagta ccaccatttt
     5761 ctctttcttt tttaaatagg accattgctt cgtcccataa ttttttagta ttttcagtag
     5821 caccagctaa gaaagagtca tttccctcat atggagaata gtttaattgt ataaactctc
     5881 taacatttat ttctttagtc catcttcctg ttttaaatcc ttgccatgca ttcattaagt
     5941 atttcctcct tagatttaaa tgataaataa tactattaaa attaattttt atatacttaa
     6001 tttattttat atattttttt aaaaatatat tttctaatat aataatatca tattattcgt
     6061 atcctgtata cataaaaaat aaaaaattgt cgctttaaag tataaaacag attatatgtt
     6121 aataatgtgg tgaaattata cccatatata aataaaataa aacaaataaa tagctaatta
     6181 aggagaaaat attttttatt ttttgtttag gataaataaa ttttattatg gaaaaaagaa
     6241 aaagcataca tcttcacaaa ttgaatattt atttaatagg gggaggtgat agattgttta
     6301 tcacagtggt attattttta gtaggtttcc tattaattac taaaggcgca gatatcttta
     6361 taaattgcac tgtagagata ggtaaaaaaa cgaatatatc agaaattata cttggagcta
     6421 ctatagttag ttttgcaaca acattacctg aatttacagt gtctctactt gcttctattg
     6481 atggtcatac tactatgagt ttaggaaatg cagttggttc tataatatgt aatacaggat
     6541 tagcattagg gttggttgtt tttataagtc catttaatgt agataaaaaa atgttttttt
     6601 ctaagtcttt actattaata gtatccgtta tagttttaat tttattgagt ttagatggag
     6661 ttataacaag aggtgattca ttattactta taataatatt aatcttttat atggttaata
     6721 attatagaag tgtcgttgga aaatctgata ctaaaaatag aaatattaaa aataacacta
     6781 ttaaagacat atctactaaa aaagggaaaa attatagagg tttttccgta ttagaaattg
     6841 taaaaatact attacttttt gctacaggat tgataatgat gataataggt tcacaaatcc
     6901 ttatagagag tggagtaata atagctagtt ttttaaatat acctcaaggt atagtaagtt
     6961 taactataat agcacttgga acttctctgc cagagatagt ttcgtcgatt actgctataa
     7021 gaaaaaatca tcatgaaata tcagtaggaa atattttggg tgcaaacata ttgaatatag
     7081 tatcagtaat agctgtatca gcaataccaa ataacatacc aatactatct caaaatagac
     7141 aattggatat accattcatg atattattat tattaatagt aataatacca acacttaaat
     7201 ctaatagact tagtagaata caaggtatat tgatgttatt cacatatttt ctgtacatat
     7261 caattttgta tttcatgtat attatttaac tatactgttt aaaatattaa ttgcagaata
     7321 tcctaaaaaa gtatataatg gataggttaa gattaattga taacggagag gaataatata
     7381 tgaatataac aaagtttgaa gatttaccaa taagtgaggg aataaaaaaa gctatagctg
     7441 aaatggggtt tgaagaacca tctcctatac aagcacagtc tatacctgcg attttgtcag
     7501 gtaaagacgt tataggacaa gctcaaactg gtacgggaaa aacagctgct tttagtatcc
     7561 ctatactaga aacaatagac ccaaataata gaagtttaca agctgtagtg ctttgtccaa
     7621 caagagaact ggcaatacaa gtatcaacag aaataagaaa gttagctaag tattcgcacg
     7681 gaataaagac attacctata tatggtggtc agccaataga tagacaaata aaatctctaa
     7741 agagtggtgt gcaagttgta ataggtacac caggacgtac aatagaccat atcaatcgta
     7801 aaacattaaa gatggataat gtaaaaatga ttatattaga tgaagcagat gaaatgctag
     7861 atatgggatt tagagaagat atagaaatga ttttaagtaa gattccagaa gaaagacaaa
     7921 caactttctt ttcagctact atgccaagag gaattttgga attaactaag agatatcaaa
     7981 aagacccaga gcatataaaa gttgttagaa aagaacttac agtttcaaat acaaagcaat
     8041 actatataga aactaggtca tcaaataaat tagaagtatt atgtagatta gtagatgtat
     8101 atgaccctaa actatctgtt gttttctgta atactaagag aaaagcagac gaattggtag
     8161 gagatttaca agcaagagga tattttgcag atgcattaca tggtgactta aaacagactc
     8221 aaagagatat agtaatggat aagtttagaa atggtacaat agatatactt gttgctactg
     8281 atgttgcagc tagaggtata gatgttgatg atgttgaatg tgtattcaac tatgacttac
     8341 cacaagatga agagtattac gttcatagaa ttggtagaac tggtcgtgct ggtagagaag
     8401 gtatgtcatt tacttttgta tttggaaaag aaatgagaaa gatgaaagat atagaaagat
     8461 atactaaatc taaactaata aagcataata taccaacaat aactgatgtt gaagagaaaa
     8521 aagtaggtac tttctttgca caagttaaac aaactataga agaaggtcac ttaactaaac
     8581 aattacaatg gttagaaggt ttctgtaatg atgaagatta tgcaatggtt gatatagctg
     8641 ctgcacttgt aaaactatct ctaggtgaag aaatgaaaga agaaataata gaagaaaaac
     8701 ctagaagaga gcgtggagat agaaaaggtg gtactggagc taaagatggt atgattagat
     8761 tatttatcaa tattggtaga aatcaaagag tacaagcaaa agatatagtt ggtgccatag
     8821 ctggagaagt tggaatacca ggtaaagtag taggtactat agatatatat gataagtata
     8881 cttttgttga aatacctaaa aaagatgcaa aaacagtaat tgaaaagatg aaagatatta
     8941 agataaaagg taataagatt aatatagaaa aagcaaacaa gaagaaaaaa taacgtatag
     9001 tatacaaaac tataattaga aaatatataa aataatatat tggatattta aaaagcctga
     9061 tgtgttttca aaacagatag tagataatct gtaagagtta acagcataag gcttttcttg
     9121 ttttatatga tgttataatt ttccagattg ttgtgaaaaa tgtatattag ttcctttttt
     9181 tcaaatctga tttgaaaaag agaatattaa ttatctatat agtttagatt ataataaaaa
     9241 atgtaagata acaaagggga gaaaaagatg tcaatgactt atatggaccc tagggttttt
     9301 ttgattttag aaatggtgtt agaagaagac acaaatactc tagaacaatt ttcaaatgtt
     9361 ttgggtgtta gtactaggac tatcagaaac tatataaacc agattaatcg agaattaaaa
     9421 ggtggtattg ctaaaatagt taaaaatgaa aaaggtacat atagtataag aattgaggat
     9481 gaaagtaaat taattgagat tgtaaatttt aatagaggaa agctatcaaa ttttataaat
     9541 ttaaattcac ctgatgaaag aataaattat gttcttgata ttttaactat gaccaacaat
     9601 ccaattacca tagatgattt ggctgaagag atatgtatag gaagaacaac attaataaag
     9661 gatttaaaaa aagcagacag aattttagct gaatatgatt tagaattaaa aaggaagcca
     9721 aatataggta tgattttagt tggtgaagaa atggatataa gattattaat ccttgataga
     9781 ttgtatgaaa attatattga tgtcttggaa aacgttaatc agataaattt agttaaagat
     9841 tatgatattg aatgtcttag agatgaatta aaaaagttat ttaaaaaaga agaactctac
     9901 ataacagaac aggtcttgag agatgttgaa agatatataa tcatgtctgt cttaagaaat
     9961 ctaaatgggt acaaatttga aaagatagat aagagatttg aagtgataag atgttcagat
    10021 gaatatactt taggattaaa gctcaaagct ttattggaaa agatatttaa tatagtttta
    10081 aatgaaaaag aaaccatatt tttgacaatg ccattaatag gtagaaaagc tcctagtaca
    10141 aaattagcac ttagcagtat aaagataaat gatagcgtaa aggaagttgt agatgaagta
    10201 actagttttt tattagagac ctcagggatt gattttaaag aagataaaaa gcttatagaa
    10261 aatttggaat atcacttgta ttttgcactt aatagaatga gattcaacat aagggtaaaa
    10321 aatcctcttt tacaagaaat aaaggaaaga tatacatttc catatagtgt tgcaaagata
    10381 gctgcaatga taattgagga aaagtttgac ttgaaaatat gtgaccacga aataggatat
    10441 atagcattac attttggaag ttattttgaa aaaaattcaa gaaaagttct aaatgtaaat
    10501 agggtagctg ttgtttgtgg aacaggtctt gggactgcac aacttattag aataaagata
    10561 ggaaaaatac ttggagaaag tgtagaagta aatacttttt ctgatataga agctaaagct
    10621 aatttagatt tattaaagta ttatgattta gtatttacaa cagtagattt caaaacagac
    10681 ttagatgttc caatttttag agtaaatgcg ttatttaatg aagaaagtat aaagaaagag
    10741 atagaaaatg caatactttt gaaaaatagt ataaatctta agtctataaa aaagattgaa
    10801 aaaaatatcc cttttatagg taaattaatt gaagaggata agttctttat attgaataag
    10861 agtacgttta tggaaaattt agaagatatg ttagaaaaat tagttcataa aggaattatt
    10921 gatatagagt tcaaaaaaaa ggtattaaaa agagaaaaga aatcttcaac agcattagat
    10981 aattatatag cattgcctca ctcagttaat actaatgggg aaaatcttta tctggcattt
    11041 ggaatattag aaaaaccagt gatttgggat acaaaggaaa ttagaataat aatattgatg
    11101 ataattccag ataagaatgt agatagcaca gacctgataa taaaaagtta tgaagaagta
    11161 cttgaagtag ggagagataa aaagatggta gaagaacttg ctaaggtaaa atcatttgat
    11221 gaattttata aaataattac caagaggagg taataatatg gacccagtaa tttttattat
    11281 agcatcactt attacagtgt atttattaaa tatttttctt ggatacttac agcttaaaga
    11341 ttttaataaa aattatattg aactaaaaag aaaaggaaga gtggcaattg gcagaaaaaa
    11401 aggaagaatt tcatcaggta caattgtgtt gatacttata aatgatgatg gtgtgattgt
    11461 cgagactaga aaaatgcaag gtgttacagt attggcaaga gtaaagtcat ttgaaggttt
    11521 ggtaggaaag agcttagagt atatagaaaa atcagattta actgtttaca ataaactact
    11581 taaaaaagca atactagatg cagtcaaaca gtacataaca tttgagaaaa ataaatgtga
    11641 agatacaaat aatctgagta ctgatgaaga gtgtaggatg gaagtagttt aaaaaaatat
    11701 atttataagg aggagttaat atgacgcaat tttttcaagt attagcaagt ggtgcagata
    11761 gttttatgaa tttatttagg gcaggtgggc aacaatttgt aggttttgta acagatatag
    11821 taccactttt agtagcatta ttagttacaa tgaatgcaat tatcaacttt attggtacag
    11881 atagagtcga gagacttgca aaaaaatgtt ctgcaaatat atttactaga tatttgatat
    11941 taccagtcct tggtacattt gtatttgcaa acccaatgac actatcatta ggaagattct
    12001 tgccagaaaa gtttaaacct agttactttg cagcagcaag cttttcatgc catacaatga
    12061 atggattatt cccacatatc aatccaggtg aactatttat atatctagga attgcaaatg
    12121 gtataacaac tttgggattc tcaacagcag atttagcagt tagatatttg ttagtgggta
    12181 tagttgcaaa ctttattaaa ggtgtagtta ctgactatac aacaaaattt gtagagaaac
    12241 aacaaggaat agcattaaat tcagatataa agattggata attactttaa ggtataaata
    12301 aatttttcaa ataaaaagat agtgtataga ggaggataaa ttatatggca tttaacaaag
    12361 ttaaaatagt aaaaggttct ggaggatggg gtggaccatt gataatagaa ccaacagaaa
    12421 agaagaataa ggtagtatat gtgactgggg gagcaaagcc agaaactgca gtaaaaatag
    12481 ctgaacttac aggatgtgag ttaatagatg gattttcaca gggtgttaaa gatgatgaaa
    12541 tcgcatgtgt aataataaat tgtggtggaa cattgagatg tggtatatat cctcaaaaga
    12601 aaataccgac tataaatata atgaaaacag gaaaaagtgg accattagca atgtttataa
    12661 aagaagatat atatgtttca ggggtaaaac caacaaatgt agagcagtta taattgaggt
    12721 agtaggaggg aagaaatatg gcttatgata ctaaaaagaa gataagtgag cagagtcaag
    12781 gaattgttgc aaaaataggt atagcactag gaaatgtgac aagtactctt tttcaagctg
    12841 gtagggatac tatagataca ttaatacata ctatactgcc ttttatggct ttcgttgcag
    12901 tcctaatagg tataataaat caatcgggta taggaaattg ggtagccaat ttacttactc
    12961 cattaatcgg aaatgttgga ggtttagttg tcttatcaat aatatgttca tttccgttgt
    13021 tgtctccgtt tcttggacca ggagctgtta tagctcagat agtgggggta ttggttggta
    13081 cagaaatagg aaaaggaaat attccaccaa gtttagcatt accagcattg tttgcaataa
    13141 actcacaagc agcttgtgac tttataccag taggtcttgg tcttgcagaa gctgaacctg
    13201 atacagttca agtaggagtt ccgtcagttt tatattctag atttttaaca ggtgcaccaa
    13261 ctgttctatt agcatggtta gcaagctttg ggttatataa atagaagaat actttataaa
    13321 taattagaga gaggttcagt tgatatggaa ttaatataca gtactcaaat aaataaaata
    13381 ggagataatg caaatgaatt tttacaacat aatatgtttg taactttcaa agataatgct
    13441 cctcaagagt tagaaaatta ctgttatata cattctgaaa ataatttagt aaaagaaata
    13501 cttacggatg atgtgctatc aataaataat gttgattaca aaattacttc tgttggtgag
    13561 ttagtaaacc aaaatctttc agaattaggt catataacat ttaagtttac aggtgaaaaa
    13621 gatggagcaa ttggaggaac cttatatctt gaagaaaaag aaatagcacc tattggcgta
    13681 ggtactatac taaaaataat tagaaaataa aatttaaaac gaatctaatc atattataag
    13741 taaattatag ataaaagtca atttaactaa tcaagttaac ttttaatgtg ataaaaaagg
    13801 ggaaatgaaa atgagaaaag tggctttagt cgttggagga ggaagaagtt taggtgaata
    13861 tttatctaaa catttagcta aagtaggtta tgatgttgct attgcagatt taaatgaaga
    13921 caacgcaaaa aaagtagctg aagaaataaa aactgaattt ggttgtaaat ctgtaggata
    13981 tagttgtaat gccatagatg aaagacaggt tataagtact gtcaaagcta ttgaaaatga
    14041 ttttgataga atagatttat tagtgtacaa tgcaggagtg gctgtaagta aaaaaataac
    14101 tgattttgga tacaaagatt ttaaatttat tgtagacgta aatttaaatg ggtactttct
    14161 atttgcaaga gaagtatcta aagtcatgat agcaaaaggt attgagggta gaataataca
    14221 gataaattca aaatcaggta gagtgggaag caagcataat tcaggatatt cagcaggtaa
    14281 atttgctgga gtaggactta ctcaaagttt agcgctagat ttggcagaac ataagataac
    14341 tgtaaatgca ctgatgctcg gtaatttact tgattctgat atgtttgaaa gtttaattcc
    14401 tcaatatgca gtaaaattgg gaatacctga atcagaagtc aagcaagttt atatagataa
    14461 agtaccttta aaaagagggt gtagatttat agatgttgca aatatgctta cattttatgc
    14521 ttcaaaggaa gctgaatact gcactggtca atctataaat attactggtg gacaagttat
    14581 gtaaggtttt tatggagtgt ctcaagatga actctttagt tcatgaggca cttttttgta
    14641 taaaattaaa tatattttca gcatttaaac aatgaaaaag aggtttatct ttattttgag
    14701 atagcctcat ttttgtaatt aaaaaacata gtgtttaagt aaacatataa gattgttagt
    14761 tttttaaaag agaatttttt aataatatta tatttatgaa gtatttttaa acttaatttg
    14821 ataaaatata gctatgtttt atatactgta gttttgaaca tagaaagttt aataaattat
    14881 agtatatatg ttaattttta ataaggtaag aaagtagaaa gaaggatatt ttatggatat
    14941 aataaaaaaa ttaagtcatg ggtgtacatt agattgtcat gactgttgta aattcaatgt
    15001 ttatactaaa ggaaataatg tagtaaaaat tgaaggagat aaaaatcatc cttatacaaa
    15061 aggttttata tgcaaaaaag ggatggccca tttagataga ttaaatcata aagatagaat
    15121 aaagactcct atgcttaaag ttgatggagt atggaaagag atttcttttg ataaagctat
    15181 agaaataatg gcagaaaagc ttacatacta taaagaaaag tatacatcaa agtctgtcat
    15241 gcattatgac caatatggaa gtgggtctgt gctaaaatac ataggagata tattttttaa
    15301 tttttatgga ggtgttagca gacataaggg tggtccctgt tggagtgcag ggatgcatgc
    15361 tcaaaaatat gattttggag tagctaagag tcatgctata gaagatatgt taaatagtaa
    15421 aagtatattt gtttggggaa aaaatccagc atatactaca attcatacta tgcaaataat
    15481 taaaaaagct aaagaaaaag ggataaagat agtagtgatt gaccccatat atacaaaaac
    15541 agctcaaata gcagataagt atgtacaagt aaatcctgga acagatggag ctttagctat
    15601 agctatggca aaaattatag tggaagataa attatatgat gaagaatata taaactcgta
    15661 tgttattggt tttgaagaat ataaaaaata tttatcttca ttggaattaa gttttttaat
    15721 tgatgaatgt ggtgttaaag agaatgatat aagagagtta gtggatttat atactaacaa
    15781 atattcaagt ataaatgttg gatatgggct tcaaaaatat aaaaatggtg gaaacacaat
    15841 aagagcaata gatgctttag gtgctataac tggtcaaata ggatttagtg gtggaggggt
    15901 aaactatgct aataaagttt atccgagtgt tataaattct gacccttaca atagccaatc
    15961 atatggtgaa gatagggagt tttatgtaag taatatcagt aaatttatag aagaatcttt
    16021 aaaaaataca tcaaataaag taaattatgc atcagatgaa cttgatatgg cattacataa
    16081 agtaaattac atatcagatg aacttgatac aacatcaaat aaagtaaatt atgtatcaga
    16141 tgaacttgat atggcattac ataaagtaaa ttatgtgtca gatgaacttg atacaacatc
    16201 aaataaagta aattatgtat cagatgaact tgatatggca ttacataaag taaatggtgt
    16261 atcagatgaa cttgatatgg cattacataa agtaaattgt gtatcagatg aacttgatat
    16321 gacatcaaat aaagtaaatt atgtatcaga tgaacttgat atgacatcaa ataaagtaaa
    16381 ttatgtatta gatgaacttg atataacatc aaataaaaca gattatatat caaatgaatt
    16441 gtataattta tctaataagt caatcaaaga taatattcct ataaagatgg ctgtgataac
    16501 taaaagtaat atgctgaatc aactgccaga tttagtagaa ttggagaggg tgttttcaaa
    16561 aattgaattt aaagtatgtt ttgatatgtt tataactgat acagttactt tatgtgacat
    16621 gtttataccg tgtacaaata cactggagag tgaagatata atttatagct caatgaccaa
    16681 tccatatata acctataatg aaagagctgt aaagccagca cataaattga tggatgaata
    16741 ttacttcttt atggagctag ctaaaaagat gggattaaat gattatcctt ttgtagaaaa
    16801 gagaacttat ttagaaaaag tgattgaacc acttaagcgt tttgacaaaa atctggatat
    16861 agaaaaactt aaaaacaatt actttactat acataaccct gttgcttggg aagataagaa
    16921 gtttgagact ccatctggaa aatacgagct ttattcagaa agtataaaaa acttgggaat
    16981 aagtccaaca cctgtatata taagcaataa atataaagaa atagaagata aaaatatttc
    17041 ttttagatta ttaactaatc atcatgcaga tactttattt agtcagcatt ttatggacaa
    17101 aaaatctata gctcaagcat atataaatca gagaatggct aaaaaagtag gtattgagga
    17161 gaaagatatt gtgatattga ggtcaaaaaa agcaaaaata aatgtacaaa taaatataga
    17221 tgatggagta ggtaattata tagtaaaaat gtatgttggt tggtggaaga aacatggaaa
    17281 tcctaatagt ttaacagaca ctggtatatc agattttggg ggtcaggtaa catataatga
    17341 aagtatggta gaaataatac gacaaaacta gttaaatttc attgaaggaa taaaaatata
    17401 attatagaat tgattaaaag ttactatttt tacaaaacta ttgaaggagg gatttttgat
    17461 ggtaaattat tccttagaac ataagaatct atacatagaa tttatgtgct cagaactaga
    17521 ccaccatgta gccaatgaaa taagggaaga aatagataac ttattaagtg taaatcaagt
    17581 aaaaaatgtt gtatttaatt ttgaaaatat taattttatg gattcttcag gtataggcgt
    17641 catcataggt agatataaaa agatttctaa tgaaggggga cgagtttcag taattaatat
    17701 aagttcacga gttaagaaga tttttgattt atctgggtta aataaaatta ttggcattta
    17761 tgatacgtat gaagaagctc taagttcttt gtagggagga gtatgtgatg aataatatta
    17821 tggaagttaa attttcggca atatctgaaa atgaaagttt agcaagagtt atagtagcat
    17881 cctttgcagc taagttagac ccaacagtag atgagttagt agatataaaa actgctgtct
    17941 ctgaggctgt tacgaatgct ataatacatg gatatgaaga agacagttct aagtttgttt
    18001 tcttaagatg tgagatagaa ggaaatacta taaagattat agttgaagat gaagggtatg
    18061 gaatagaaaa tgtggagaag gcaatggaac cactttatac atcaaaacca gaacttgata
    18121 gatcaggaat gggattcaca gtaatgaaaa gctttatgga cgatgtagag gtaagttctg
    18181 taaaagataa tgggactaga attgaaatga ctaaaaaaat aaatgtgcct aaataaagtc
    18241 caaaagtgag gttttattat ggaagtaact gttgccagag aagaaaaaaa acctcttctt
    18301 agtcatgaag agactctaga acttattgaa aaagttcaaa atggagatga agaggcaaaa
    18361 gaaatattaa tatcaagcaa tttaggtcta gttaggagcg ttgtatctag atttttaaat
    18421 ataggatatg atagagaaga tttatttcag ttaggttcta taggcttaat aaaatcaata
    18481 tataaatttg acccaaaatt taatgtaaaa ttctctactt atgcagtacc aatgatttta
    18541 ggtgaaataa agagatactt aagagatgat ggtatgataa aagtatcaag gtcgttaaag
    18601 caaatagctg taaaggcaaa gatggaaagt gaagctttaa ctaaaaaatt aggtagagag
    18661 ccgagtatag aagaattagc taaagcaata gatgtagaaa aagaagattt agtaatggct
    18721 atggaagcta atttcaatgt tgagtatttg caaggagtta ttcatgaaga ggaaggttct
    18781 ccaatatgcc ttattgataa aataagcatg aaaggagaaa gtgaagaaga aaaagtagtg
    18841 gataacatat tgttaaaaga catacttgga agactggata agagagagcg tcaaataata
    18901 gttctcagat actttgaaga catgactcaa agtgaaatag gagagatgct caatatatca
    18961 caagttcaag tatctaggat agagaagaaa gtattgtcta agttaaaaga atatatatcg
    19021 tgataatact tatgatatgt agaataacaa aatataataa atatattact aaaataaaat
    19081 gtaataactt tttatgttaa gtttttaaat tattaaaaga tattaaaata ataaaaagca
    19141 ttaaaaaagt aaaaatataa aaacaaggtg tctttatgga aaaatagatt ttttctttat
    19201 taggcatctt ttttattttt tgaccaaact ttagatagtc aaaataaata ataggtttat
    19261 ttttttaaaa ttaacaaata atactttaga gaggtgttta gtatggataa aaattataaa
    19321 aaatatgtag accaaataag cccaaaacct acatatttaa aaaactatac acttgcattt
    19381 attgtaggtg gaattatttg tatgataggg caagcaataa atgatttgta tatgaaagtt
    19441 ggaggtcttg ataaactagg tgctagttct gcaacatcaa taacattaat atttataggg
    19501 gcatttttaa ctggattagg agtttacgac ctaataggaa aaagagctgg agctggttct
    19561 ataattccaa taacaggttt tgctaactca atagtatctc cagcaatgga gtataaaaga
    19621 gaaggctatg tattaggtgt aggtgcaaac ttatttaaaa ttgcaggacc agttttagtt
    19681 tatggtatag gttcttctat tttatgtgga attatttatt atatttttaa gatgttttag
    19741 atgattttta tttaatttaa attttattaa ttatgaagaa ggtataaaat ctgaaaatag
    19801 ctactaaagt aactaattga aaaataattg tgcagattta attaaaattt ttaattaata
    19861 ttaaaaattt gaatagtaaa atgcaaaatt aaaaaggtgt gaaaatatga aaaataaaag
    19921 aattggaaaa agaacagtca agttagaaaa taaaccaacc ataatatcaa ctggaactat
    19981 agtaggtcct aaggaaggtg aaggaccact aaaggactat tttgatatga ttatgactga
    20041 cgatttatat ggtgaaaaga cttgggaatt agctgaaagt aaaatggtag agactgcatc
    20101 tcaacaagct atccaaaagg caggtaaaaa attgtcagat gtaaattata tgctaggtgg
    20161 cgatttaata aatcaaatag ttccagcatc atttgcagca agagaactgg ctattccatt
    20221 tttaggtata tatggagctt gttctacaat ggcagaaggt ctttgtatag gttctatgct
    20281 tatagatggt ggttttgctg atttagtact gtcaggtaca tcaagtcact attgtacagc
    20341 agaaagacaa tttagattcc cacttgaact tggaaatcaa aaaccaatga cagcccagtg
    20401 gacagttaca ggagcaggaa gtgtattatt agctcctaat ggagacggtc caaaagtaaa
    20461 gtatgttact gttggtaaag taatagatga gggtattgat gatggtaata atatgggacc
    20521 agctatggct ccagcagcaa tagatactat ctactcttac tttgaagata ctaatgatga
    20581 cccaaatagc tttgatataa ttgctactgg agatttaggt actcttggaa aacagatagc
    20641 agaagacttt ctaaaagaaa aaggtgtaga tatatcaaaa gtttatactg actgtggtat
    20701 agagatgttt aaccttaaag aacaagatgt ccactgtggt ggaagtggat gtgggtgttc
    20761 agccacagtg tttgcaggat atatatatga taagttgaga aaaaaagaat ttaacaaagt
    20821 aatgcttgta tcaacaggag cactactttc accgacaagt acactacaaa agcagacaat
    20881 accatgtgtt gcacatgcag tagtaatagt aaatgagtag gaagtgatat aatatgttaa
    20941 tggactatgt aagagtattt attgtaggtg gaattatatg tttaatagcc caagtaatga
    21001 tggattattt caaaatgcaa actccatatg ttatggtaac gtatgtaaca actggtgtag
    21061 ttttaacttt tctaggactt tatgagccat tagttaagtt tgcagcagca ggtgcaacag
    21121 ttcctataat aggatttgga tattcacttg ctacaggagt aataaaatca attcaaagtg
    21181 atggtttttt aggtattttt ttaggaggta caactgctac agctggtgga atagcagcag
    21241 ctatattctt tggatatatt atgtcagtag tatttactcc aaaagcaaaa ccataaatct
    21301 ttattaaaag aataataagt ttttgaaata ataaattttt ataaagcagg tggatatctt
    21361 ttagattcca cctgtttttt atataaagaa gcgtttacat aattgaagtt gacaattatt
    21421 tacaacatag tgtagtatta tattatcaaa ataaaatgaa gtgaagtagg ggataaagat
    21481 ggtaactatt aaagatattg caaggaatct aggtatcagt tattcaactg tttctaggtg
    21541 cttaaacaat aatcctaatg tatctgaaaa aacaaaaaag aaagtggtag aagaggcaaa
    21601 tagactaggg ttccatttta atgtaaatgc aagaaattta gcaaaaaaag aaactaacag
    21661 gataggggta atcttttcaa ataattttaa tcatcaagat actagaaagt tttttagtga
    21721 tataatggat agttctataa attctattga gactaataaa tacgatttta taatacagcc
    21781 taataataac attagtggtg atagtaatgt atataaaatg gtaaatggac aaatggttga
    21841 cggattagta attgtgtctc aatcaataaa aaaagaagag tatgattttt taaaagacaa
    21901 taattttcca catgtattta tatattttaa accatctttt ataggagaag tagataattt
    21961 cttttgggac gataacgtgt atggaggtta tatagctacc aagcatttaa ttgaccatgg
    22021 tcataaagat ataataacag taacttcaga tgataagagt tcaaaaatgc atgaagatag
    22081 aaccaaggga tatttgaatg ctatggaaga agcaaattta aagacagaag tcattaaatc
    22141 taaaatggat tttgaatctc aagttgaatt tttagaaaaa tacattgata aaataaaaaa
    22201 agcttcagct atatttgtac aacaagatgt accagcgatt tctataatac aagagttaaa
    22261 aactacttat ggtataaatg taccagaaga tatttccata atagggtata ataatattga
    22321 attaatatca tattttaggt cgcatttatc aacaatagat gacccaagag agcaagttat
    22381 aaaaaatgga gtagatagtt tagtaaatat aataaataaa aagagtacag aacacattcc
    22441 gagaaaatta tatccaaggt taataatacg aaatagcgtt aaaagaataa agtagtaagc
    22501 ttttaagtat caaacgttta ttaaaaatat tatagattat gtgaagtttt ttaacaaata
    22561 atataaataa aaattaaaag gagtag
//
LOCUS       DBGJFU010000059        22322 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.059, whole genome shotgun sequence.
ACCESSION   DBGJFU010000059 DBGJFU010000000
VERSION     DBGJFU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.059"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(128..1318)
                     /locus_tag="ACOIAS_002111"
     CDS             complement(128..1318)
                     /locus_tag="ACOIAS_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HGZ2701821.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1578..2009)
                     /gene="dut"
                     /locus_tag="ACOIAS_002112"
     CDS             complement(1578..2009)
                     /gene="dut"
                     /locus_tag="ACOIAS_002112"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGZ2701822.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2122..4350
                     /locus_tag="ACOIAS_002113"
     CDS             2122..4350
                     /locus_tag="ACOIAS_002113"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HGZ2701823.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4461..4946)
                     /locus_tag="ACOIAS_002114"
     CDS             complement(4461..4946)
                     /locus_tag="ACOIAS_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2701824.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5198..6421
                     /locus_tag="ACOIAS_002115"
     CDS             5198..6421
                     /locus_tag="ACOIAS_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGZ2701825.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6425..9559
                     /locus_tag="ACOIAS_002116"
     CDS             6425..9559
                     /locus_tag="ACOIAS_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HGZ2701826.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSREKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9581..10891
                     /locus_tag="ACOIAS_002117"
     CDS             9581..10891
                     /locus_tag="ACOIAS_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HGZ2701827.1"
                     /translation="MNKKMSKIVAISCGVGIITANLIPVYADNNTSSINKVDNSILSD
                     SNKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFN
                     TENGLVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11180..11425)
                     /locus_tag="ACOIAS_002118"
     CDS             complement(11180..11425)
                     /locus_tag="ACOIAS_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2701828.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11639..14266)
                     /gene="ppdK"
                     /locus_tag="ACOIAS_002119"
     CDS             complement(11639..14266)
                     /gene="ppdK"
                     /locus_tag="ACOIAS_002119"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HGZ2701829.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14327..15169)
                     /locus_tag="ACOIAS_002120"
     CDS             complement(14327..15169)
                     /locus_tag="ACOIAS_002120"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HGZ2701830.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15176..15814)
                     /locus_tag="ACOIAS_002121"
     CDS             complement(15176..15814)
                     /locus_tag="ACOIAS_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2701831.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(16250..17542)
                     /locus_tag="ACOIAS_002122"
     CDS             complement(16250..17542)
                     /locus_tag="ACOIAS_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGZ2701832.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(17579..17935)
                     /locus_tag="ACOIAS_002123"
     CDS             complement(17579..17935)
                     /locus_tag="ACOIAS_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGZ2701833.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(17970..18329)
                     /locus_tag="ACOIAS_002124"
     CDS             complement(17970..18329)
                     /locus_tag="ACOIAS_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGZ2701834.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(18398..20371)
                     /locus_tag="ACOIAS_002125"
     CDS             complement(18398..20371)
                     /locus_tag="ACOIAS_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2701835.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(20593..21606)
                     /locus_tag="ACOIAS_002126"
     CDS             complement(20593..21606)
                     /locus_tag="ACOIAS_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGZ2701836.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(21619..22164)
                     /locus_tag="ACOIAS_002127"
     CDS             complement(21619..22164)
                     /locus_tag="ACOIAS_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGZ2701837.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         7650 a         3728 c         2881 g         8063 t
ORIGIN      
        1 aataaaaagc aggtatcaat caaatagtaa catgtattat actatacatt actatcaata
       61 ttgtacctgc tttcatatta taagcaataa agaaaaatct atttattagg ttttataata
      121 aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag ctactattta
      181 tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata tctccatttc
      241 ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta tcaaagaata
      301 ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta ctttggtctc
      361 tagatactct tggtatacta actcctacag attttttcat aacgtactgc gtgaatccag
      421 aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca agtagtgtct
      481 ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa taagatggaa
      541 ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat ccagtttgat
      601 tgccataact tatttttgtc catcctccac tttcagaaat acttccaacc tcagtaccat
      661 aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg ttcaatgact
      721 ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca gcattttcat
      781 ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct actctaccat
      841 tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca gtcccatatc
      901 ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt aatccttttg
      961 ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta cttccaaggc
     1021 tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat ttaactttta
     1081 cccaagaacc ttcttttcct acgtactcaa ctttatcacc tttgtaaagt ttccccattg
     1141 attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt attgttctgc
     1201 tttctatact cacactctct acttcagact cattagcata tgctaaattc cccatagtag
     1261 gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta aaattcataa
     1321 ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat aaaagaaata
     1381 attgacacta tcaaaatatc aattatttct tttatattaa tattattaca aatttgtaac
     1441 tatttgtcta tagtaatatt tgcttaccat aaattaccat gtataaattt taatctttaa
     1501 tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc tatatttttt
     1561 aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc tctttcggag
     1621 tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac tatcatttga
     1681 gcaattttca tatgcttttc aaccttaaaa tcatttttac catgatttat taatataatt
     1741 ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac agtaacagaa
     1801 tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt aggaagctct
     1861 atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac ctcttctata
     1921 gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat agcatcatca
     1981 tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt ctataaatat
     2041 tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata taattattag
     2101 tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc tgtggcaaaa
     2161 actatagcat catttttagg tgcaaagacc agacaagatg gttactttga aggaaatgat
     2221 tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa ggattatgat
     2281 aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc tgaggataag
     2341 tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa aaagcttttg
     2401 aatagaaaag atgtagaata tgtaataaat gccactgata atgaccgaga aggagaactt
     2461 atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag aatcttggta
     2521 aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga tgaagatgaa
     2581 atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt gattggaata
     2641 aatttcactt ctgttgctac attgaaatac ggaaatggga aactattaaa cataggtagg
     2701 gtaattcttc caacagttaa attagtctat gatagagata tggaaatact aaactttgtt
     2761 cctaaaactt attatgaaat agaagggcat tttaaagctg aagctggtga atataaaggt
     2821 aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa taaaattata
     2881 gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat gtcaaaggaa
     2941 tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc taagtattcc
     3001 aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg taatggtaag
     3061 ggtggataca taacttaccc tagaactgat tccattttct tagaagaaag tttggcttca
     3121 aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa taaaattaaa
     3181 ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag tgctataatt
     3241 ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta tgatgccata
     3301 aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac agaaattaag
     3361 actgaagtag ataattgtac tttcttaaca aaaggtaagg tgcttaaatc aaaaggatac
     3421 cttgaggtat ataataagga agaaaaaaac gatttacttc cacttgtaaa taaagatgat
     3481 gttgtagatg tgttagaaat aaaaccatta actaagcaaa ctactccacc taaaccgtat
     3541 actgaggaca cacttttaaa ggctatgaaa aattgtggta aaaatgtgcc tgaagaagat
     3601 acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga tgtgttaaag
     3661 aagataagtc aagtgggcta tgtaaagaaa aaaggtaaat cttattctat aacagacctt
     3721 gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga ttatactgga
     3781 aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa agaatatctt
     3841 actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga tgcttcaaaa
     3901 aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac taaaacaaaa
     3961 gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat atcaaaggat
     4021 tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg caaatgcccc
     4081 atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa tcataaagaa
     4141 tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa gaaagttaac
     4201 aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc tttaactgct
     4261 aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga tactggctat
     4321 ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat agcagtagta
     4381 taataatcta tattatatta ctgctatttc ttttcttatg aagtttcaac ttttttaata
     4441 gtgactttta caatcacact ctaacctaat ttctttacta tttctgataa ctttaatgta
     4501 tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt tactagagat
     4561 tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatctgt taattcaacc
     4621 ctaagaatcc ttctatcttt tgggtcagga aatttattga ctaaaccatg ttccactaat
     4681 ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc tatttgagaa
     4741 attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga tggagccatt
     4801 ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt ttttaacaaa
     4861 tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa agagtccgct
     4921 attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat aatataataa
     4981 aacattgttg tttaatatag tataaattat aaactattaa taaatttaaa acaagaaaaa
     5041 gttattgact cataataatt ataaatttat aatcaaatta gagaattata aattgccaat
     5101 aaagggaagt tttttaatta gtctaaaact aaacttaaat tataagacac tttaaattct
     5161 acaacaagct agtgtgcaat atataatagg aggagatatg aaaagatatt taatagtttt
     5221 cttaatgtgt agttcactgt ttttagttgg atgtggaaaa acagaagaat ctgaacctga
     5281 gaaaccaata gcagtttctg ttcaaaaagc aataggtgga gaaattgaaa acacaaattc
     5341 tttctcagga actacaaagg ttaaagatga aacagctgtt actgcacaga ctgttggcac
     5401 agttcaagaa gtgtatgtaa aacttgggca aaatgtaaga aaaggagatg aattattgag
     5461 tataagcagt ccagagctag aaaatagtgt aaagcaaagt aaagcctcac tagatttagc
     5521 aaaagcaagt tattcaagtg ccactggtgg ttcattagag gcacaagtaa atcaggcaaa
     5581 aacagctctt gacaatgcaa agattcaata tgaggagtct caaagaaatt atgacaataa
     5641 taagattctt tatgaacagg aagtcattag tttagaccaa tttaaaaaaa tagaattttc
     5701 tttagagcaa acaaaacaac agttagattc tgctcaaaga gcatacgata cagcaacttc
     5761 aaaaagtata cctcaagcta aagcacttgc taaaaaacag cttgaccaag ctcaagtatc
     5821 atataattta gcaatgagta atttagacaa attaacatta acatctccag tagatggtac
     5881 aataacagct aaaaactttg attcaaagga aatgataaca caatcacaac cagcttttat
     5941 tatttctaat cctaatatac tagaagttga tttaaatgta gcagaatcag atataggtaa
     6001 atttaagaaa gatggaaatg tagatgttat aattgaagac caaaggattt taggaaaaat
     6061 agattatgta ccttcagtag ttgaccctca gacatcttta tatcctgtta aagtacttgt
     6121 aaataataca aacaacaaat ttaaggcagg tatgtctgct caagtaaact taagtgttga
     6181 aaaagaaaat ggtgctgtta cagtacctaa aaaagcaata tttgaagaaa atggtaaaaa
     6241 atatgtatac atagcaacaa aagacaatat agcaaagaaa cacttggttc aaactggaat
     6301 tgtaacagag gataaaatag aaattaaaag tggtgtaagt gataaagata cagtgattat
     6361 cggagggata agtcttattt ctgacggaac taagatattt cctgtagaaa aggagaagta
     6421 gactatgaat ttaacacaga cttccgttaa acgtcctctc acaatcataa tggtcttttt
     6481 agtattaata gtttttggtg gtattggata taaaaaaatg tcaataaatc taatgcctga
     6541 tatagaaata ccagttgtaa tggtcatgac tacttggaca ggtgctggtc ctcaagacgt
     6601 tgatgaacaa gttagtcaaa aagttgatga aagtttatct gccgtttcaa atgtaaaatc
     6661 tactatatca agttcacaag aaagtgtttc tatggtagtt gctcagtttg aatttggaac
     6721 taatttggat gaaataatga atgatgttag gtctaaagtt gatgcattac agaccagcct
     6781 cccagatgat gcagctaaac caacagtttt aaagctggat atgaatgcac aggctatagg
     6841 tcaacttgtc atatctggag gaaatgaaaa tagttcacaa gccttaagaa aatacgctga
     6901 agatgtaatt cagcctaaaa tagaatccat agatggtgtt acttcagcag accttaaagg
     6961 tggagaaaaa gcacaggtta atgtaatagc tgacccagct gttctttcaa attatggagt
     7021 tagtttatcc actataaaag gtgtcttatc ctctagtaat aaaacattcc cttatggttc
     7081 aattactcaa ggtgaggata aaatagtttt aagagcgatt gataaattag aatccctaga
     7141 tgatataaaa cagattcaaa ttcctgtaaa aggaggaaat acagttagat tagataatct
     7201 ttgtagtgta gattatggat atgcagacaa agatagtatt tatagataca atggaaaaga
     7261 cagtttggta atagatattt ctaaacaaca agatgccaac actgtaaaag ttatgcagag
     7321 tgcatacaaa tacgtagatg aattgaataa ggaaaatcct caatttaaaa ttaaaattgc
     7381 taatgataca agttcataca taagtgaatc aataaacagt gtaatgagca acttgcttat
     7441 aagtgctgtt atcgcattta ttgtaatatt tgctttccta aaaagtgtaa gagcttcact
     7501 agttgttgca gttgcaatac caatttctat aataggtgct atagcaattc tctactttac
     7561 tggtgaaagt ttaaatctta ttactgcaag ttcattggta atttcagttg gtatggttgt
     7621 tgataatgct acagttgtaa ttgaaaatat atttaaatat aggaaaagtg gtagattgag
     7681 tcttgatgat tgtgctattg agggaacaag aactgtaaca aatgcaatac ttgcatcaac
     7741 attgaccaca atagcaatat tcttacctat catgtttaca gaaggtatag ctaaaataac
     7801 ttttggggca ttaggtaaaa cactaatagc ttcactagca ttctccttta taggtgcaat
     7861 tacactcgtt cctagtgtat ttgcaaaatt aagcagagaa aaaaatagtc aaaagatgca
     7921 agaaaaagat agtcctatat ttgataaggt aagtgaagca tataaaaaat tattaagtgt
     7981 atgtttgaga catcaacgct tagttgtaat atcaagttta cttatttttg tagcttcctt
     8041 atttggaatg ggatttatag gtatggactt tatgcctgca gcagacaagg gtgaattatc
     8101 tatttctata gaacttccaa aaggtcttag cctagaatcc aatgattact atgtatctat
     8161 gactgagaaa aaagtctcag atattccaga aattaaaact ttaataacaa ctttgacatc
     8221 agattcaaat aatagtaaag caagtatagc aattgaatta gaatctcaaa aaaatagaaa
     8281 aagaactact gatgaaatag aaaaagagat aattgataga gtggctactg taccagattg
     8341 taaaataaat gtaagtcaaa acagtagtgt tatgggtgga gctggaggag cagacttcac
     8401 attagaaatg aaaggtcctg atttagatgt attaaaaata ctgggaaaag aagctgaaaa
     8461 agaatttagc tctataaaag gatttagaaa tgttgaaact tctcttacag atacaacaca
     8521 agaagctcaa tttactatag ataaacataa ggctcaagaa tatggtgtaa ataccgctga
     8581 aattgcaggc atgttacgta cagcaataaa tggtgactcc gtaaccactg ctactattga
     8641 tgattacaaa gtggatgtaa acctaaaatt taaagcagat actattgata gcttagaaga
     8701 tataaagcaa ctaaaagtaa actctgcaac tggaaaagaa gttcctcttg gtgccttttc
     8761 agatataaaa atggcagatg gtctacaatc tatatctagg actgatggag atttttctgt
     8821 aaatataact gctacacttg acaatttaga tgctggtact gctacaaaac tagcactaga
     8881 aaaaacgaat aaaatggtaa ttcctaaaga ttactctata ggtgttggtg gtgaaacaga
     8941 aatgatgaat gaatctatgg caagtcttgg attttcaatg gtcatagcta tagcattagt
     9001 ttatatggta atggtagctc agtttgaatc atttagtaaa ccatttataa tcatgttctg
     9061 tgttccattt gcattcgttg gagttgtgct ttcgcttcta gttagtggtc aaagtttaag
     9121 tactgtcggt atgctaggta taatcttact tatcggtatt gttgttaata atggtatagt
     9181 tcttattgac tacatagagc aacttagaaa aacaaatcgt aacaatgatt taattggttt
     9241 ggtttcaatt ggttgtgcag caagattaag accagtctta atgactacag caacaacaat
     9301 tttaggtatg ttgccaacag cactatctct tggtgaaggt ggagaaacta tgcaaccact
     9361 agccatagtt ataatgggtg gtctaactgt atctacactt ataactttgg tgcttatacc
     9421 tactatatac ttaatatttg ataaatggga aaataagttt tcagacaaaa tgcctaaaat
     9481 atttggtaag ataacagata aaataggaag tataaaacgt aaaataataa agaaaccaaa
     9541 attgaaagct aaagtttaaa tagaatggag ggtgattgta ttgaataaaa aaatgagcaa
     9601 aatagttgca attagctgtg gtgttggtat aataactgcc aatttaatcc cagtttacgc
     9661 agataataat acttcttcta taaacaaagt tgataattct attttaagtg actcaaataa
     9721 aagaacatct gaaaaggaaa ttttgactgc tagagaagct gttagtgctg cacttaaaaa
     9781 tagtgaaaaa ttaaaaatga aatcagaaga aataaaaatg ttaaaagaaa agttagaagt
     9841 acaagatgag tttgatagtt ttactggaag tgacaactcg tttccatacg accaaattgg
     9901 acttcttaaa aatcaaagtg aacaggctaa aggctttatg gaggaccaaa tagcaaatga
     9961 tattactaat aaatttaacg atttagtttc aagagaaaat gagctagata aaataaaaaa
    10021 caatcttgaa ataaaaacta aagaaattaa agatatgaaa ttaaaaaaag atttagggtt
    10081 agtaacatct ttagaaactg aatcagctga acttgaactt caaacactac aaaatactca
    10141 aaaagctaaa cttcaagaac taaaaaacaa tcaagactat tttaagttat taactaatat
    10201 agatttaaat aattatcaat tggataaaga atatagattt gaatctttta gagtaggtgg
    10261 ctcagttgat tcatatatgg aaggtaaggt taatgaatat ttaaaatacg accaattgat
    10321 tttagaacgt acagaagaaa gctttaatga taaagatgag aacaaagcag atttaccaga
    10381 tagacctaac cttacaagac cagttgctcc tacaaaacca gagcaaggta acctaactga
    10441 tgatgaatac aaggttctta tggataagta tgaaaaagat tatgatagat ataaaaaaga
    10501 agttgaagca tacaatcttg aaagacagac ttatgctgct ggccttacta cttatgcaaa
    10561 ttatcttcaa caaaaattta atactgaaaa tggtctagta acattagaag acagtaaaaa
    10621 agctcttaaa aaagggttaa ttgatagcta tgctcaatta cttgctttag aagatacaat
    10681 tcagattact aaaaaacaac ttgatttatc agaaaaacaa ttaaaaaata ctaaattaag
    10741 atatgattta ggattaatta ctctaactga ttataaaaaa caagtggtaa gtaatgaaga
    10801 tgctaaaaat agctatgaca ctcttattgt gaattataac tcactaaaaa atggtataga
    10861 aaaaccttgg atacttaata ctggaaaata attctggtaa atagaaagat tactttaaca
    10921 tacgtttgat taaaaaatag ttatgcatac tcgtggcttt agtcatggaa gattcagaag
    10981 gcaaataata gaaatagatt agtaaaaata tatttactta taaaactgta tacttttata
    11041 gaggctatct caaaatagag ataagcctct tttttattgt ttaaattata aaaatatatt
    11101 tgattccata caaaaagagt gccttctgaa ctaaaaagtt cattttgaga caccctcaaa
    11161 agcttaactc taaatttaat tattttttat caaatgtttc atctatttta catctcacca
    11221 tgttaacaaa ttcagaaata ttatatcctc taagtttgca tccacattta ggacaataat
    11281 ccagtttcct agataccaaa gccctacaac taggttctgg acatcttatt tttagactct
    11341 catctgctac tttaaatcca catcgtgggc atatactacc tggacgtgat gatacttcct
    11401 ttccacattt aggacacttt gtcaacttta tcccctccag taaactcaat ttaattgata
    11461 gttgaaattt ctctctacaa taatatatgc aatataatta aatacgtttc acttacaact
    11521 atataagtta aatttttata ataaaaatcc cctatattca atttacaaat acttaatttg
    11581 atacattgaa caatagggga ttttattttt atttagttac atattttgca gtctattatt
    11641 atctaggatt ttttatagat gcttgagctg ctgcaagtct agcaattggc actctaaatg
    11701 gtgaacaaga tacatagtta agtcctacac tataacagaa ctctactgaa gatggttctc
    11761 ctccatgctc tccacatatt cctagtttta attctggttt aacttctcta cctaatttag
    11821 ctcccatctt aactaactta ccaacaccat tttggtctag tacttggaat gggtcttttt
    11881 ctaatatttc ttcatctaca tattgaccta agaatttacc agcatcatct cttgaatatc
    11941 caaatgccat ttgagttaag tcattagtac caaaactgaa gaagtctgct tcttgtgcta
    12001 tttcatcagc agttaaacaa gctctaggta tttctatcat agtacctaca gtataaggaa
    12061 cttctatacc ttcttctttt attatagcgt caacagtttc tactatcatt tttcttatta
    12121 tttttaactc atttacttct ccaactaatg gaaccattat ctctggagaa actttaacac
    12181 ctgctttgat tgcttctata gcaccttgta ttatagcttt agattgcatt acacatattt
    12241 caggataagt tattgcaagt ctacaacctc tgtgtccaag cattgggtta aactcatcta
    12301 aatcaactat tctcttctta atatcactta cttttattcc catagatttt gaaagttctt
    12361 ctatagtctc atcatcatga ggcaaaaatt catgtagtgg tgggtctagt aatcttatat
    12421 taactggttt tccatccata actttaaata actctacaaa atcctctctt tgcattggta
    12481 atattttttc aagagctttt tctctttgtt ctactgtatt agataatatc atttctctaa
    12541 ctgctggcaa tctagcttca tcaaagaaca tatgctcagt tctacataga cctatacctt
    12601 ctgcaccaaa ctctatagct gcacgagcat ctctaggatt atctgcatta gttctaacca
    12661 tcatacattt gtgtttgtct acccagttca ttaatttttc aaaatttcca gttaaagata
    12721 cttctgtttt cttaacatct cctaggtaca catttcctgt agaaccatct atagaaatgt
    12781 attctccttc ttttataact acatcatcaa gtgctcttat ttctttatta aactcatcaa
    12841 cttttatttc tccacatcca gcaacacaac atttacccat acctctagca acaactgctg
    12901 catgtgacgt cattccacct ctagctgtaa ggattccttc tgctttaacc attccttcta
    12961 tatcttctgg agatgtctct agtctaacta aaactacttt ttctcctttt tcatttgctt
    13021 ttactacatc atcagcattg aaataaactt taccacttgc tgcacctggt gatgctggta
    13081 aaccttttgc tataaccttg gcttctttta atgccttatc ttcaaatttt ggatgtaata
    13141 attggtctaa ttgattaggc tcaattctca tgatagcctc tttttcatca attattccag
    13201 cttcaactaa atcaaccgct acatttattg ctgctgctgc tgttctctta ccatttctag
    13261 tttgtagtat atataatctt tcattttcta tagtaaactc tatatcttgc atatctttat
    13321 aatgaccttc aagtatatga gtaattttta caaattcatc gaatacagct ggcattacat
    13381 ctgcaagagt tgatatattt tgaggagttc ttatacctgc aacaacgtct tcaccttgtg
    13441 cattcattaa aaattcacca aataatttat tttctcctgt agctggattt ctagtaaatg
    13501 caacacctgt accactagtt tctcccatat taccaaatac cattgattga atatttactg
    13561 ctgttcctaa gttatgagct atatcattca atttacggta tacgattgca cgaggattat
    13621 tccatgatct aaatacagcc tctattgcaa gcattaattg ctcttttggg tcttgtggga
    13681 aatcattttt tatttctttt ttataaattg ctttaaattc ttttactatt tcttttaaat
    13741 catcagttgt aagttctgta tcaactgtgt atccctttgc ttcttttact ctgtctaata
    13801 cattttcaaa tttatatttt ggaacttcca tagcaacatc agaaaacatt tgtatgaatc
    13861 ttctgtaact atcatatgca aatctttcat tttgtgtagc ctctgctaag ccaacaacac
    13921 tattgtcatt aagacctagg ttaagaattg tatccatcat tcctggcata gagaatactg
    13981 caccagaacg aactgatact aataatggat tcttattgca tcctaatgtt ttatttaagt
    14041 ctttttctaa agtagctaaa tgctcttcta tttcttttat tatctctgct cttatacttt
    14101 cattgttaac ataataatca ttacatgctt ctgttgttat agtgaatcca ggagggacag
    14161 gtaagcctat cttagtcatt tctgctaaat tggcaccttt acctcctagt aaagatttca
    14221 tgtctttgct tccttcacca aaactataaa catacttagt ttccattgtt aaatccccca
    14281 ttaatctttt tattttaatt tatatcattt ttattaatag acattattaa atttctattt
    14341 ctttgtatat ctttagacca ttttctttca ttatgtctaa aattatacct gctgtttctt
    14401 ctattgcctt atttgaaacg tttattactg ggcatccaat tcttttcatt atctcttctg
    14461 aataatctaa ctcctgaagt attctttcta aattagcgta gtttgcttta ctggacaatc
    14521 ctaaagcttt taatctttgg gttcttatct cattcagctt ttcaggtgaa ttagtaagac
    14581 ctattatttt tttagtatct atctcaaaca cttctttagg aattggtatt tctggaacta
    14641 atggaacatt agctactttt atatttttat tagctaaata catactcaaa ggagttttag
    14701 atgttcttga tattcccact aaaattatgt ctgcttgaag aacacctcta gggtcttttc
    14761 catcatcata tttcactgca aattctatag cttcaactct cttaaagtaa gattcatcta
    14821 attttctaat tataccagct tctcttttag gttttcttcc tgttctcttg cttatttctc
    14881 ttagtatatc tgtcattaaa tctatacaac taagaccttc tctttcgcaa tatctttcaa
    14941 caatactgca taattcttca tcaactaatg tatatacaat taaagcgttt tcagcttttg
    15001 cattctccaa agtttcaagt agtactcctt tattaatcat gtatggaaat ctttttattt
    15061 cataagtttc attcacataa aactgagaca tacatgcctt tgcaacttgt tgtgcagtct
    15121 cccctactga atctgatact gcatatataa ttaggttttt caccagcata cctccttaat
    15181 tgtcaactat ttctaagaat agcttagtta tgttggtttt tgatattctc ccaacaactc
    15241 tcatatgatt tttgtcttct cccttatatt caacaatcgg aatcgaatct acttcatgtt
    15301 ctattatttt tcttgaagct aataaaacac ttgctccttt agttaaagtg actacatttg
    15361 gagttcttgt cattatcata cctattggca ttttatttat atcagaacct cctatagttg
    15421 cttttagcaa gtcttttctg gaaacaactc cacataactc ttcattctca tctataataa
    15481 ctatactccc tacatcagat aaaaacatag tgactattac atcatatata ctttcatcct
    15541 tcttagctag tacaggcatg ctcattatat cctctactgt tttttctttt acacttttac
    15601 caattaaatt tatttcacta actcctgaat aaaaatatcc aacttttggt cttgcgtcta
    15661 atattcctgt cattgttaat atagctaaat cagatctaag tgtagcacga gtcacattca
    15721 agctggaggc aatactttca ctcgttattg gttcattttc cttaactata tctataattt
    15781 ttagttgtct ttcattaagc tgaataataa tcaccttctt tcattgtgct atacttttta
    15841 tcttttcgca tcttatatag tatattatat aactatatat accattctgt aaacagattt
    15901 tttattgaat acgatatatt ttttactaag ttctctcgct taaagatatt tattatatat
    15961 taatggggga tatttcttta attaatagaa ctatattagt attacagtta atattataat
    16021 acataattgg tcattttaat tcattttata ttaaaatttt taataatatt atttcaatgt
    16081 gaatataaaa ttgcagaact aattcaaaat aatttttacg actacaaata acctataata
    16141 tacttttaaa gaaagtatcc taacaagaat atataaaaat ttaaaagggt tgatttaaaa
    16201 ataaccatca acccttttta ctaatatcta tttatattac catcaaatct tacccaaata
    16261 ttttttcttg caattgtctt aaatgatata aagtagtatc tttttctatc tctaccatat
    16321 cgtaagttat aacttcacct tttttaatat cttttacaac tttagtcttt ttacttatca
    16381 aaccaatagg tacagcattc atagcctttg ctacatcgta tttttctata gtaccatagc
    16441 atgtatatgc tccatagcca tccataaact cacctttttt caaatctttc ttagctatag
    16501 tcataacttc tgctactagt ccagctttag gtactattgt agccttatgg tctaagactg
    16561 ccatagctgc tgataaagga gtctctaaac tacataaatg atatggtcta tataatatgt
    16621 aattaggtcc atcacccata cttaaatatt ttagttcatc attaactgct tttaatttat
    16681 gggctactac tacgaaaact ccaggtgcta taccatttac aaaatcaact acaccataat
    16741 tatctaatac tccaccttcg ctttttaatg catatttctt aggtacttca ttaactgtag
    16801 cttcaatacc atgaccacct cttacatcag gcacaaatcc agttgcatta cacattaaag
    16861 ccatttctac cattgtttta gttccttctt taaaagaagc tatcatatga ggtgaagctc
    16921 cttttctttc tgcttcttct ctaactgtat caggattaca atctaaatct aatttattat
    16981 ttttaccttt acctatcact ctaacttcaa accccattgc atctgcaaaa tcaaataatt
    17041 ccattactgc acctggctca tctcctgcag aacctgtaaa tattactcca gcatcatctg
    17101 ctaatttttt aagtattggt ccaacaacac aatcagtttc agcatttaac ataactatat
    17161 gttttttatt taaaatagag tttaatgcta tctctgcacc aattgcaata cctccagttg
    17221 catctataac tacatctatt gacttagttt tacaagccac aagaaaatca ttacatgtca
    17281 atacttttcc ttcttgtaat tttatttctg catccttttc atttgttaca ttatctataa
    17341 tttcttcatc tattcctgca tcagtaaaag ctttatgagc caactccaaa tttatatcta
    17401 caacaataga agcttcaata cctttcatat ttgccatttg gcttaccatt ccatttccca
    17461 tatgcccagc tcctataaga gctactttta tttttttacc ttcctcttcc aattttgcaa
    17521 gttttctgtt taaatctaac ataccaattc ctcctgttat aataatttta ttttcaaatt
    17581 aagaaatagt tattaaatct ccaatagtta tatctggatt tcctatttcc acatgtattt
    17641 ctcctggtaa atttacttca cattttccat caaatttgat tgtacaatgc ccaatttctc
    17701 taagagtctt ctgagcaata tcaccaactg aaactataca ataatctcta ttccctatag
    17761 taagtgtatc tcctatttca acatcacttt ttaacgttgc tattgaatga attacagata
    17821 tatcctttaa gtcgtcatca ttaatggttt catcatatat aataagacaa ttgctttcat
    17881 ccattaactc tttaactaaa ctgcctatcc cacttatctt aacttcatat ttcatgacat
    17941 tcctcctgat aatttatatt tatattgagt taattaaaag aaacatttat tttttctttt
    18001 ataaatttta tagcttgtac aatactctct tcatctttaa atttttgctc tgcactatat
    18061 atatctaatc cttctatttc ttcaatctct ttaaatttag ccattactgt acgtcctttt
    18121 aaaatttctc caaactttat aaacccatct gaatctgaag atataatggc tatacttcct
    18181 ttagaaaaat agcttttctt tttgcctgta gacactatat cacccttttt tttagctcta
    18241 tttaaagttt taaaatacaa tttattctgc ttcattataa atatagtatt taaaatataa
    18301 gcaacaaata aaatcactaa taatttcata tttatacacc ttccaaaaat cacattattt
    18361 ttaaatctgt ataacaacta taaatgctgt acaatagtta tataaggttt ttaagtaaca
    18421 cctttttaaa ttctaaaaaa gttctacatt tacttatctt attcgttaat tttacatctt
    18481 ggcctatctt taaaacttct tcataaattt ttactaataa gtctgagtct atcttattct
    18541 cacatggaaa cattgtcata agaattattt ttatacttct atttgcatat tctattggtt
    18601 cttctaaaat tccaactgcc attaaaaact tatctgattt ttcatttaca gaatgaggaa
    18661 ataataaacc tttatcaaat actgtgggag atttttcttc tctattgact atattttttc
    18721 taaacttatc atcaatgcat cctaaatcca taagatggtc catcattttt tctaatgaag
    18781 caagtatagt cttttcattt aatatcataa atttatcttc atctaacaaa ttatttatta
    18841 gtaaatttgt agaattagta gtgtcaatat tcccttctct taggcacaat actgttttta
    18901 attgttctct tagtttgttt tcatcaaata ttgtatttat ttttaatact attgtattta
    18961 tatttgatgt gtctaaatca atagttgtaa aaactatatc atattcatct aagttaatat
    19021 ttctcaaatt aaaacttgaa agagtatcaa ttttaggatt atttcctatc agtttttcta
    19081 ctcttatttt caatagtttt gctgttccta gacctgtact acatataatt gctatttttt
    19141 ttattgaata gaatctactg ctatttcttt ctaaatatcc actgaaatgg attgctatat
    19201 atccaatctc atcttctgaa acttctaagt tatacttttt ctctattact tttcctgcta
    19261 tttgtgctaa tttaaatgga agtatataat tttgcttaat ctcttctaac aaaacatttt
    19321 ttaactttat attaaataat agcctgttta aagtaaaact aagatgatat cctagaccta
    19381 ctcttaattc atcctcattt ataaaaatac ccatatcgac atatatttga ttaaatatat
    19441 tttccatcaa ctcatcaatg tttttactaa ttctactact atttaaagca cattctgatg
    19501 ctggtgcatt tccactaact aacggtatag ttaaataaat aatttcttca tcatttaaag
    19561 tacattcaaa cattcctgat attatatgtt ttatttccct agcaataaag tattcatcat
    19621 atgatttaag taaatcaaac tttacatcat aatcttttac tttcttttct tcttttactc
    19681 tatagactaa aactactatg tagttaataa cttctcttaa catttcatct gtaacataaa
    19741 aattattttt tttaaataaa gtcaatagtt tttcttctaa atttatgatt tcttcttttt
    19801 tgattccttt aaaatacttt gaattactga aatctttcct tgagttttta tacaactgat
    19861 ttaatataaa cagtctaata tcaagttcat ttccatttaa tttcatacct gtattctgtt
    19921 ttttaatcaa gtttaaatta tatgaagcca aaactttttc tacatactgg aaatcattta
    19981 ctaaagtagt tcttcctaca cacatctcat cagctaaact atcaagtgta ataaatccat
    20041 ctagttctat caagtaattt agtataaact ctactctatc ttcttttgaa tttaatgaaa
    20101 atacattttt tctattcata tcaataatct tattgagtat ctgctcatct tttatttcta
    20161 gtttatagcc ttgacccttt atatatttga ttactgctat atcattacct aaatctttat
    20221 tcaattgttt tatatacgtc cttacacttc tagttgtgac attcaattca tttgctattg
    20281 agttaatact cacaaactct tttttactac acataatcaa tatcttaaat atcttgcggt
    20341 caatagaagg aattttctca gttacactca ttttgtatct cctccttgat ttcattctag
    20401 tacaaatttg aaaccaaatt aatacataaa atttcacttt aataaaggaa aatttagtat
    20461 taatctaact cgataattac agctaattaa tagtataaaa gctagtcttt cattaacaaa
    20521 aaatcttgac tagcttaaat ttatatttac aattttatta attaattcca agccttatat
    20581 tgacattaaa tattatctca atgcaaatac tgcaactcca tatgcaataa taacagctat
    20641 tggaccagtt attaaacgag agtaaaataa agctggaaca ccatactcta cagtttctgg
    20701 ttctgcttct cccaaactta atcctactgg tacaaaatca catccagctt gcccatttat
    20761 tgcaaataaa gctggaagtg ctaaatatgc tggtattgca ccaagaccaa tttgtgtacc
    20821 tacaagagtc cctactactt gagctataac tgcaccaggt cctagtattg gtgataagaa
    20881 tggaagtgtg caaattactg atataatcaa taatccacca atattacctg caagtggaga
    20941 tataactctt gcaatccagt ctcccagtcc agatgctaaa ataattccca taagcatact
    21001 tacaaatgcc ataaatggta ttacgttatt cattaccatt tggatagtat ctcgacctgc
    21061 atcataaaat tttgatacta cttgccctgc cccttgacca atagaaagta ttatgttctt
    21121 tttagcatat tcaccttgtg cttttgactt aacctcatca taattatcag gctttctaat
    21181 agaactctta ttttcgcttt tattttcttg agatttttgt ggcatatctt ccccatctac
    21241 aacacttata cagttaggat taacatctga tacataatat tcttctgtta taaattttgc
    21301 taaaggacct gtttttccaa ctggatttac atttattgta ggtatttttt tctttggata
    21361 aactccacat ctagcagttc caccacaatc aataataact actgctactt cttcatcaga
    21421 aactccagtt ttaaatccat ctacaatttc acaacccagt agttctttta tttttaatgt
    21481 tacttcatgg ataccaccag cagtcattga cagtatctta tttctattcc cttctttttt
    21541 tatatacaaa ggacctcccc agcctgaagt tcccttttct atttttaata ttttattctc
    21601 ctccatttta tctacctcct aagccttatt tgttgctcta ttttttcttg ttagaaaagc
    21661 atacattttt tctgttacta tacctcttat aaaaattaca attagaccaa ctataaaata
    21721 ccacaatgcc aatgtagtag tttctttacc taaagtagtc aaacctgcag atattcccaa
    21781 ccatacaaaa agttctcctg catttgcatg tggaaaaaga cctgtaattg gatgtacaaa
    21841 tgaaactgct gcatcataaa atcctgcttt gtgctcttct tttaaaaatc taccaaatgt
    21901 atagcacata ggatttccca agaaaaatac tgatactaat ggaagtagaa tatatcttgt
    21961 aaaagagttc tttcctagct tagaagccca attattaact ctttcctcac caattatctt
    22021 aatcaaagag ttaatagcta ccattaatac aattagcgtt ggaagagtac ctgaaacaag
    22081 acctataaat tgttctcccc ctttctgaaa caaaccgata aaccactctg cacctttact
    22141 tagacctgta ataacgtttt ccataaaatt ataacctccc tataatatct ttaaattttt
    22201 aattttactg cttaccataa attttacaaa ttacatcttt tttctgtact atatgctaat
    22261 attgtaaatg tttttttatt aatatcattt taatataata tttttgataa ataaatacat
    22321 ct
//
LOCUS       DBGJFU010000060        22303 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.060, whole genome shotgun sequence.
ACCESSION   DBGJFU010000060 DBGJFU010000000
VERSION     DBGJFU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.060"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            275..733
                     /locus_tag="ACOIAS_002128"
     CDS             275..733
                     /locus_tag="ACOIAS_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2701838.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            794..1069
                     /locus_tag="ACOIAS_002129"
     CDS             794..1069
                     /locus_tag="ACOIAS_002129"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2701839.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1128..2402
                     /locus_tag="ACOIAS_002130"
     CDS             1128..2402
                     /locus_tag="ACOIAS_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGZ2701840.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2532..3584
                     /locus_tag="ACOIAS_002131"
     CDS             2532..3584
                     /locus_tag="ACOIAS_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGZ2701841.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3584..4615
                     /locus_tag="ACOIAS_002132"
     CDS             3584..4615
                     /locus_tag="ACOIAS_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGZ2701842.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4707..5519
                     /locus_tag="ACOIAS_002133"
     CDS             4707..5519
                     /locus_tag="ACOIAS_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGZ2701843.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5522..6442
                     /locus_tag="ACOIAS_002134"
     CDS             5522..6442
                     /locus_tag="ACOIAS_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGZ2701844.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6617..7063
                     /gene="rpiB"
                     /locus_tag="ACOIAS_002135"
     CDS             6617..7063
                     /gene="rpiB"
                     /locus_tag="ACOIAS_002135"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HGZ2701845.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7093..7758
                     /locus_tag="ACOIAS_002136"
     CDS             7093..7758
                     /locus_tag="ACOIAS_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGZ2701846.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7868..9370
                     /locus_tag="ACOIAS_002137"
     CDS             7868..9370
                     /locus_tag="ACOIAS_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HGZ2701847.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9682..11271)
                     /locus_tag="ACOIAS_002138"
     CDS             complement(9682..11271)
                     /locus_tag="ACOIAS_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HGZ2701848.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSNI
                     NYYYREFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVKF
                     LENVINLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIYE
                     NIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFM
                     YIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVI
                     GDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDML
                     KNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQS
                     IVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDM
                     FIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENA
                     HMEIIIDADLFKVMLTFRKVN"
     gene            complement(11240..11956)
                     /locus_tag="ACOIAS_002139"
     CDS             complement(11240..11956)
                     /locus_tag="ACOIAS_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2701849.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12495..13295)
                     /locus_tag="ACOIAS_002140"
     CDS             complement(12495..13295)
                     /locus_tag="ACOIAS_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGZ2701850.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13609..14340)
                     /locus_tag="ACOIAS_002141"
     CDS             complement(13609..14340)
                     /locus_tag="ACOIAS_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HGZ2701851.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14327..15022)
                     /locus_tag="ACOIAS_002142"
     CDS             complement(14327..15022)
                     /locus_tag="ACOIAS_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2701852.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15024..15815)
                     /locus_tag="ACOIAS_002143"
     CDS             complement(15024..15815)
                     /locus_tag="ACOIAS_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701853.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            complement(15825..16637)
                     /gene="modA"
                     /locus_tag="ACOIAS_002144"
     CDS             complement(15825..16637)
                     /gene="modA"
                     /locus_tag="ACOIAS_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2701854.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            complement(17272..17472)
                     /locus_tag="ACOIAS_002145"
     CDS             complement(17272..17472)
                     /locus_tag="ACOIAS_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGZ2701855.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(17697..17873)
                     /locus_tag="ACOIAS_002146"
     CDS             complement(17697..17873)
                     /locus_tag="ACOIAS_002146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701856.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(17927..18016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(18196..18396)
                     /locus_tag="ACOIAS_002147"
     CDS             complement(18196..18396)
                     /locus_tag="ACOIAS_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2701857.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(18398..18748)
                     /locus_tag="ACOIAS_002148"
     CDS             complement(18398..18748)
                     /locus_tag="ACOIAS_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HGZ2701858.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            18910..19143
                     /locus_tag="ACOIAS_002149"
     CDS             18910..19143
                     /locus_tag="ACOIAS_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701859.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYNHSQVERWVNI
                     LGECALKIKKIFYTIWFKLDVINYMMITKYSVQ"
     gene            complement(19576..19776)
                     /locus_tag="ACOIAS_002150"
                     /pseudo
     CDS             complement(19576..19776)
                     /locus_tag="ACOIAS_002150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20135..20629)
                     /locus_tag="ACOIAS_002151"
     CDS             complement(20135..20629)
                     /locus_tag="ACOIAS_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HGZ2701860.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
     gene            21434..21811
                     /locus_tag="ACOIAS_002152"
     CDS             21434..21811
                     /locus_tag="ACOIAS_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2701861.1"
                     /translation="MKEEILIEKLLRVELIVMEYLWKKDTLMPKKKIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            21916..22290
                     /locus_tag="ACOIAS_002153"
                     /pseudo
     CDS             21916..22290
                     /locus_tag="ACOIAS_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         8615 a         2708 c         3333 g         7647 t
ORIGIN      
        1 tttgtaaaaa acaaaaaaat catttctcta acaaaaaaaa ccacaaaaaa tataaaaaag
       61 tatggctagt taagtgatta acaagtaaaa attaaaataa aaatcaaaaa aaatttatca
      121 atgaaagtca gctaaaatcc tggaagagag cattattttt gggaaaacat tttcttttgt
      181 ttcacaaaaa aagccacttt ttggcacaca acatgcttta tataaagtca agaaaaacta
      241 aggtataaga caaaggcaat taggaggtgt aaatatggag tttataaatt ttgtaagtga
      301 taaagttata gaccttaaaa tggattgtaa aacgaaagaa gatttcttta aagaaataca
      361 taataaagtt tttgatttag gctttgtaaa agaagaattt ggagaaaaaa tattagcaag
      421 agaaaatgta tttccaacag gtttaaattt aggagattat ggagtagcaa taccacatac
      481 agatgcagaa tatattaaag agcaatttat atcagtctgt acttttaatg aaccagtagt
      541 gtttagttct atggaagacc aagatgaaaa agtacctgta aatttggctt tcgtattagg
      601 acttaatcaa ccacacagtc aattatctgt attaacagaa ttaatgggaa ttatgcaaaa
      661 taaagagtta gttgataagt tgatgagttc tactgataaa gaagaagttt taaagacact
      721 aaagtcttta taaaaataaa aaagtactaa aaataaaaaa tataaaaata aaaattatta
      781 ggaggaaata aaaatgaaaa aaatattagt agcatgtgga gcaggaatag caacatcaac
      841 agtagtttgt gacaaggtag agagattagt taaggaaaat aatatacaag cagaagttat
      901 acaatgcaaa atagctgaat gtgcaacaaa gcaagaagga gcagatttaa tagtatcaac
      961 aactatacta ccaacaactt ataatatacc agcaataaaa gcaacagcat atataactgg
     1021 agtaaataca gcagcattag ataaaaaaat attagatgct ttaaaataat cctatcatag
     1081 atacattgaa ataatatttt ataaaacaga aaaaggagga tttataaatg gataaattat
     1141 tagcagtagt acaatacatt ttaaatatgg gaccgacagt aatactacca ataacaattg
     1201 tagtactagg aatgatattt agaattaagt ttacaaaagc tttaaagtca ggtcttacaa
     1261 ttggtatagg atttgtgggt ataaacttag ttgtaaacct attaacatca acactagggc
     1321 cagcggctca aggaatggtt gaaagattcg gacttaattt aacagtaata gatgttggtt
     1381 ggccaacaac agcatcagca acatgggctt caccagttgc accaatatta ataccagttt
     1441 gtttattagt aaacttacta ttattatact tcaataaaac aaagatatta aacgttgata
     1501 tatggaatta ttggcacttt atagtagcag gtgcagttgg atatatagta acaggaagtt
     1561 atgtttgggc tatattctgt gcaatactaa tggagttatt agtactgttt attgcagaaa
     1621 agacagcaaa aaatgttcag gagttttatg atttagaagg tatagcatta ccaactggtt
     1681 caacagcttc atttgctcca cttggatatt tagtaggtaa atttgttgaa aaaataccag
     1741 gaataaataa aatacaagca gacccagact ctatacaaaa aagatttggt atatttggag
     1801 aaccaatgat gatggggatt atactaggta taatacttgg agcacttgcg ggatatgatg
     1861 tagcaggaat attcaatgta ggtatatcaa tgggtgcagt aatgttactt atgcctagaa
     1921 tggttagaat attaatggaa ggtttaattc caatttcaga atcaataaga gatttcttac
     1981 aagcaaaata tggagacaga gaactttata taggtctgga tgcagcagta gcaactggac
     2041 atccagcagt tatatcaaca gctcttatac ttgttcctat aactttgttc ttagcagtta
     2101 tactaccagg aaacaaagtt ttaccatttg gagacttagc gacaatacca ttttatatgg
     2161 catttatagt gtgttttaga aaaggtaata tagttcaatc agttataaca ggaacaatag
     2221 taatagctat atcactttat atggctacaa actttgcaga tgttcataca caattattac
     2281 aacaagcaaa tatgatgcca gaaggaacaa caatggttac aagtatagat acaggtggaa
     2341 acttgctaaa atggggaata ttgaaattat ctcaattagt ttcttcattt ggaatattct
     2401 agtatgtagt taatagagat tactcttttt tagtaaagta tgcgaaaatt taacatttag
     2461 cagctactaa aaaagagtaa tattttttat aaatttttaa aaatacaaaa tacaagagga
     2521 ggagatttat cgtgaaagca gcagtattac atggcacaaa tgatatgaga tttgaagata
     2581 tagaaataaa accatgtgaa agtgatgaag taaaaataaa agttatggca gcaggaatat
     2641 gtgggtcaga tcctccaaga gtattaaaac attggaaata tccagttcca gctataccag
     2701 gacatgagtt ttctggagta attgctgaag ttggtaagga tgtaaaaaat gtaaaagttg
     2761 gagatagagt agttgcaatt ccttttatac cttgtaatga atgtgaatat tgtaaaagag
     2821 gattattttc attgtgtgat gaccatggaa tgttaggagc taaaagtttt ggagcttttg
     2881 cagagtatgt aaatataaaa gctacaaatg tacttccaat aggagatatg aactttgaag
     2941 atgctgcaat gatagagcca ctagcagttg ctatgcatgg agtacttaat ataggtgttc
     3001 aagttggaga cactgtagct gtaatgggta gtggaacaat ggggcagttg gtaatacaag
     3061 gacttaaaat agcaggggca ggaacaatta tagcagttga tatatctgat aataaattaa
     3121 gagaatcaaa ggaattaggg gctgatataa taattaatgc taaagatatt aacccagtag
     3181 aaaaaataaa agaacttact ggtggaaaag gtgttgacat agctcttgaa tgtgcaggtt
     3241 caaaaataac tcaagaacaa tgtttactta taactaaaaa gaaaagtaag ataggatttt
     3301 tagggatagc atattcagat ataactttat cagaagaagc atttgaaaat atatttagaa
     3361 aagaactaga attaaaaggt ttttggaact cttattctgc accatttcca ggacaagaat
     3421 ggactaaggg aatcaatcta gttaatgaag gaaagataaa attaaaagaa atggtatctc
     3481 atagattctc acttgaagat acttacaaag catttgaaat gataagagat agaaaagaag
     3541 aatttaacaa aatattaatt ttaccacaag gggttgaaaa ataatgaaag cagtagttaa
     3601 aactaaacca ggctatgata atgtagaagt tttagaagta gaagaaccaa aagctacagg
     3661 agacaaggtt aaaataaaag tagaatatag tggtatatgt ggttcagata tacattcatt
     3721 taaaggtgaa tatgcaaata taaaagcccc agtaacatta ggtcatgaat tttctggaat
     3781 tgttgtagaa gtaggagaag atgttaaaaa tataaaagta ggggatagag ttacatcaga
     3841 gactactttt gcaacttgtg agaaatgtat atattgtgaa actaaagatt ataacttatg
     3901 cccaactaga aaaggtattg gtactcaagt aaatggaagt tttgctgagt atgtattatc
     3961 aagagaagaa agcatacatg tacttcctga aaatgtatct ttattatcag cagcccttac
     4021 agagccacta gcttgttgtg ttcatgcagc tcttgaaaag actactatag aaagttctga
     4081 tacagtttta ataataggac caggaccaat tggtttatta ttagctcaag ttgttaaatc
     4141 tcaaggtgct acagttataa tgtctggtgt tacaaaagat aaggaaagat tagaaatagc
     4201 taaaaatctt ggagtagata gaactgtaaa tgtaatggaa gaaagtttgg cagatgtagt
     4261 gaatgaaatg actgatggaa atggagtaaa caaaggattt gattgttctg ggtttatgcc
     4321 agcagttaac gaagccttaa gactgcttag aaaaaaaggt actttcgtac aagttggaat
     4381 ctttgctaaa aaattaaatg aaatggacca agaagcaata atccaaagag agttacaata
     4441 tataggaagc aggtctcaaa agccaagttc ttggattaca gcattagatt taatggcaaa
     4501 tgggaaagta aatacagaag ctttagttac tagaactgtt ggtttagacg aatttaaaga
     4561 aggtattaca gctttaatga acggggaaga aataaaagta gcagtaaaat cttaaaaaaa
     4621 catttgtatt attttaaaaa aaattgtaaa atctactgta ggataaaaaa atgtaaggtc
     4681 ttaaaaaata acaggaggaa gaaagtatga gcactcttag agatcattct aataatatta
     4741 gaaaaaacat aataaaaatg gttgcagaag ctaaaagcgg acatccaggt ggttcattat
     4801 caatagctga tatgttgaca gtattatatt ttgataagat gaacgtatgt gcagaaaatc
     4861 caaaaatggc tgatagagat agatttgttc tatcaaaagg tcatgcagca ccagctttat
     4921 atgctacatt agcagaaaaa ggatttttcc cagaagaaga attaataaca ctaagaaaat
     4981 ttggttctaa gttacaaggt cacccagaca tgaaaaaagt agcaggaatc gatatgtcta
     5041 ctggttctct tggtcaaggg ctatctgcag caaatggtat ggcacttgca ggaaaattag
     5101 ataacaaaga ctatacagta tatactatat taggtgatgg agaaatccaa gaaggtcaaa
     5161 tatgggaagc tgcaatgaca gcatcacatt acaaattaga taatttaatt gcatttgttg
     5221 acttaaatgg attacaaata gatggttcta atgaagaagt aatgaatgta agtccagtag
     5281 atgacaaatt caaatctttt ggatggaatg taatagtaat aaatggacat tcttttgaag
     5341 aaataggaaa tgcagttgat gaagctaaaa aagttacagg aaaaccaact gtaataatag
     5401 ctaaaacagt aaaaggtaaa ggtgtttcat ttatggaaaa taatgctgca tggcatggaa
     5461 cagctccaaa tgcagaacaa gcagagcaag cattgaaaga tttagaagga ggtctatagg
     5521 aatgggaata gcaactagag aagcttacgg acaagtgtta aaagaacttg cagaaaataa
     5581 agatatagta gtattagatg cagatttagg aaaagctaca aaaagtatat catttaaaga
     5641 agttgcccca gatagatttt ttgacatggg gatagcagaa ggtgacatga ttggtacagc
     5701 agcaggtctt gctacttgtg gaaaaatacc ttttgcgagt acttttgcaa tatttgcagc
     5761 aggaagaggc tatgagcaaa taagaaactc tgtagcatat ccaaatttaa atgtaaaaat
     5821 agcagctact catgcaggtg taacagtagg tgaagatggt ggtagccatc aagctataga
     5881 ggatatatct ctaatgagag gtataccaaa tatggttgta ttaaatccag cagatgcttt
     5941 agaagcaaga caagctattc ttacatctat tgattataat ggacctgttt atataagatt
     6001 aggtagagct gcaactcctg atgtaaacag tgaaaattat aaatttgaaa taggaaaagg
     6061 aactgtttta agagaaggtt ctgacataac agtaatagct acaggaataa tggtagcaaa
     6121 agccttagaa gcagcagaag aacttgctaa agaaggtata aatgtagaag ttgttaatat
     6181 atctacaata aaaccattag atgaagcatt aataaaagaa actgctaaga aaactggaaa
     6241 agtagttaca gcagaggagc acagtataat aggtggttta ggttcagctg tatgtgaagc
     6301 tttagcagaa actaaagatg tagtagttag aagaattggt gttaaagatg tatttggtca
     6361 atcaggtaca ccagctgatt tattaaaaca ctatggttta actacagaag atatagtaaa
     6421 aaatataaaa gagttgttat agatataaat atatctaaat aacaaattga tttgacataa
     6481 gtcaattaaa gctgtcttaa aaatagaaag aaatttctac aatgggacag ctttattttt
     6541 acaatacttt atttttatga taattataaa atagtctgaa agtaatcaaa aaaattataa
     6601 aaagaggtgc tatttaatga aaatatctat tggatgtgac catggtggtt atgaattaaa
     6661 ggaatatata aaggaaaatt taataaataa aggtatagaa gttgaagatg tgggtacaaa
     6721 ttcaaaagaa agtgtagatt ttccaatata tgcaaaaaag gtaagtcaaa atgttcaaag
     6781 taaaaaatct gatttaggca tactttgttg tggaacagga ataggtatgt ctattgcagc
     6841 aaataagttt aaaggaatca gagcagctgt tgtttctgac tgtttttcag ctaaggcaac
     6901 tagagaacac aataatacaa atgtactttg tttaggagaa agagttattg ggaaaggatt
     6961 agcaatgaga attgttgaag aatggttaaa ttcaagttat aatggagaaa gacatgcaag
     7021 aagattacaa atgattgatg atatagaaaa agaaaattta taaatatata atttacaaca
     7081 agggggagcg ttatgtctat tatttgtgct tcaataatgt gtgcagacca aatgaagtta
     7141 aaagaggaat tagaagctct tgaagaagca ggtgtaaaat tacttcattg tgatgttatg
     7201 gatggaatat ttgttaaaaa cttggctatg ggaccagagt tattaaaatc aataagtgaa
     7261 aatacaacaa tacctttaga catacactta gccacagaaa ctccagataa atatattgat
     7321 atgatgtctt atataaagcc aaaatatatt tcttttcatg tagaatcaag tacaaatgta
     7381 aaagctgata tacaaaagtt aagaaattat ggtatagggc cagtgttagc aataagccct
     7441 caaacaagtg tggataaaat agaagaatat ataagtttag ttgagggtat cttagtaatg
     7501 acagtaaatc cagggtttgc aggtcaaaag tttaatcttt ctgtattgga taagttggat
     7561 aaacttacag agatactaaa agattatgat aatcctccat ttatagaggt tgatggaaat
     7621 ataaataagg atactattaa acttatgaat ggtaaaaaag tagatatata tgttgttggt
     7681 acttcagctt tatttaatga taagcctcca atttcatata aagataaaat agaagaacta
     7741 aaagaaagta taaagtaacc atgtaataaa attataatat gtttctgttt aatagtaaaa
     7801 atttaggtgg aaacaagaaa ttttataata tttaataaaa attttggttg agtaaggaga
     7861 attttttatg ggagacctat ataaactaca aaatggtact gatattagag gaatagcgta
     7921 tgaaaatgat tctaaagaag ttaacctaac tgtagaagaa gtaaaaaaaa tagctaaagc
     7981 gtttcatatt tggttaaaag aaaagactaa gaaagataaa gtaactgttg ccataggaac
     8041 tgattctagg ataacaggaa gccaatttag aagtactgtt attgaaactt taactaatga
     8101 tgattgtaat gtaattgatt gtgagattgc cacaactcca gctatgttta tgacaactat
     8161 tatggatgga tacaattctg atggggcgat tatgattaca gcaagtcact taccttatta
     8221 ctataatggt ttaaagtttt tcacagaaag tggaggttta gaaaaaactg atataaaaga
     8281 gatgttggat atagctgtta aaaatgatag tgtagactat gaggaagtaa ataaaaaagg
     8341 tgaagttata gtaaaaaatt taatagaaga ttattctaat ttattgatag ataaaattag
     8401 aaaaggtgta aattcaagta aaaattatga aaagcctttt tctgggctta agatattggt
     8461 tgatgcagga aatggggctg gtggattctt tgctgaaaaa gttttacaca tactgggagc
     8521 agatacgaca ggtagtcagt ttttgaaccc agatggtatg ttcccaaatc atatacctaa
     8581 tcccgaaaac aaagaagcaa tggaaagcat atgtaaagct gtgttggata ataagagcga
     8641 cctaggaata atctttgata cagatgtaga cagagctgct attgtaggta aaaatggcaa
     8701 accaataaat aaaaatgctc ttatagctgt tatatcatca atagtattag aagaacatcc
     8761 aaaaacagct atagtaacag attcgattac ttctgaagga cttgctaaat ttataaatga
     8821 attacaagga agacatcata gatttaaaag aggatataaa aatgtaataa atgaagctat
     8881 aagattaaat agtgaaggtg aagagtgcca tttagctata gaaacgtctg gacatgctgc
     8941 tttaaaagaa aattatttct tagatgatgg ggcatattta atagctaaaa ttttaataaa
     9001 agtagcaaag ctatccttgg aaggaaagac tatagaagaa ttgatagaaa accttgaaga
     9061 agctcaagaa gagaaagaag ttagaatagg cattaataaa gaagatttta gaacatatgc
     9121 agaggatata ttaaaagatt tagattctca tgttaagagt attgatggat ggtctgtagc
     9181 accaaataat tttgaaggca taagagttaa ttgtgatgaa tcaaatggag atggatggtt
     9241 tttacttaga atatcactac atgaaccatt attagcttta aatattgaat caaataaaat
     9301 tggcggagca gaacaaattt attctaagtt aaaacttttc ttagaaaaat atgatttaaa
     9361 aggtctttag ggtaaataga aagaatatat ttatattgaa gaataaatgt aaatttgtac
     9421 ctaagattac aaatattaca aaattgtaat cttaggtaac agagatgtaa ctgattatgg
     9481 aaacactatg tgatattata gatacagaga gatgaaatat gtgatgaatt atagagaaat
     9541 ataatttaaa atgtattaat atcacaccaa aaaggtataa tcaaaaaata tgtaaatata
     9601 aaccccaaca tttagaaaaa gacctctaag gaggtttttt tcttgtttaa ataacttaaa
     9661 atatattttt atattaatat atcaattaac ttttctaaaa gtaagcatta ccttaaataa
     9721 atcagcatct ataattattt ccatgtgtgc attttctaat tcaattatat tttgagcaat
     9781 tgataaacca agaccagagc catctgtttt tctagatata tcaccccttc taaatctttc
     9841 tattaactct tctggtttta gatttagttt atcatttgaa atatttttaa acgttaatat
     9901 tatattttct tcctttataa acatatctat atagacccta gtgttaggca tagagtattt
     9961 tagtatattg caaattagat tctcaaaaac tctccaagtt tttttaccat ctatcatggt
    10021 aaaaacagca tattcaggtg tttctattat aaaatctaaa ctgctatttt gaattttgtc
    10081 ttcaaattct acaatacttt gaattaaaag ttctcttata tcggtttttg ttatattaaa
    10141 ttctacatct ccactactta gcttagatgc ttcaaatatg tcatctatta ataattttaa
    10201 tctttgtgat tttttatcta aaatatttat atagtcattt atagtctctc tgtcaaagtc
    10261 attatttttt aacatatcaa catagcttaa tatagatgta agtggagttt ttaagtcatg
    10321 agatacgttt gtgataagct ctgccttcat cctttcattt tttatattag catctacagc
    10381 agtttcaaga ccgcctgtta tattatttat acttttggct aaagtagata tttcatcatc
    10441 tcctataact tctaatttat actttaaatt gccattttct atatttttta tattttttga
    10501 tatgttattt atgtaattaa acttaacaga taaaaagttg tatatataaa aatgaaaaac
    10561 tgtgtttaca agtgtaaata aaattactat agctattgcc tcagcataat atatatacat
    10621 aaagaatatc aaaacaagat attggataat agcttgtaca actatagata ttaaaaatac
    10681 tatagctaat tttttattta tagttttttt attatacata ctaagtaagt ttgaaaagaa
    10741 tacatatgtt atagacatat cttttagatt atagttatta tatttaatat tttcatatat
    10801 tatatagact atagatataa ttataaaatt gtagattatt aaattagtgt tgtaaaacat
    10861 tttattactt gtaaatgcat gaaaccttaa ggttaatagt atcaaagtta atataagtgt
    10921 aagttctatt tttaccttag aaaaaaggtt tatgacattc tctaagaatt tgactttagt
    10981 ttgtttattt tttttataca atgtaaaaca tagtatagtc aataaaaaac ttgataatat
    11041 aatacctatg cagacaaact ctaattctac ttttgacaaa tgtacaggag taccgaaaaa
    11101 ataaaaataa gggagagcaa actctctata ataataatta atattagaaa agttattatt
    11161 caaatcataa actgcttcaa ataatataga aactaataga aacagtaata ataataaaat
    11221 aaattttgct ttagaatcat taataattaa gtttttcaat tttgtaacca atgccccaca
    11281 ccacctttaa atattttggt tcttttggat taatttcaat cttttctcta attcttctaa
    11341 tatgaacagc aatagttctc tcacaactat aagcatcctc attccagaca ttttcataaa
    11401 tctcatgaga agagaaaatt ttaccttttc gtgacataag aagtgtaagt attttatatt
    11461 caagtggtgt cattttaatt tcctcaccat ctaaagaaac tatttttgta tctgtattta
    11521 gctctaggcc accacttttt agaatataat tagtatcatt tgagttattt gtattactaa
    11581 aatcacctaa atttatatac cttctaagtt gagattttac tcgagcagta agttctagtg
    11641 gattaaatgg tttagttata tagtcatcag cacccatatt aagacctata attttgtcca
    11701 tatcttctga ttttgcagat aagagaatta ttggtatatt ccttttttct cttattttaa
    11761 gtgtagtttt aagcccatct aaattgggca tcattacatc cattattatt aatttaatat
    11821 cattttcgat tagtatgtcc aatgcttcaa gtccatcata tgcctttaaa acattaaagt
    11881 tatcaggtct caaatagaac tctatagcat caactatctc tctatcatca tctacaacca
    11941 atatattttg attcaattta ttaaccccct tgaaatttta tttttaacca atttttattt
    12001 tataccaaaa agaggtatta gaaaacagta agcccgttca ttatgaaata aaaactagta
    12061 cttttaatat attgtcaatg catatagtaa atttaaaagt atagacgccc atattaatct
    12121 gaaggtaaaa gtttatgaaa atatctaggg taatctatca cacttcttat gttattttgg
    12181 tttattaatt ttttaggttt aaacatatat taacaaatat aaataacaaa tgagtttcat
    12241 aattattaag aatttataac aaatatttat tttaatgtag tttaatataa acaggtagtt
    12301 taatgtaggt atgtataaag catttaataa ggcttatagg ttttaatgat gatatcttta
    12361 tatattttaa gagatgatga aattagatat gggtaatagt tgtatgagaa aaagtagtat
    12421 agcacaaaaa gtgctatatc acaaaatctc aaataatttc ctttttataa cttaaattaa
    12481 agaataaata tttcttataa attaggtgta tgaagtatag caggaacatc tttattattt
    12541 aataacttat caaatgattt atggtcagac ttatagccaa taccttctct atattctaat
    12601 tcatcaacaa ttttgtattg ttgacaggca tttttaacct tttcatagtc ctcagtagaa
    12661 tgagctttat aaactatctt attattaaaa gttattttat cagcaaattt aggttttaca
    12721 aaatatttag catcttttat tttaccagca ttatataaat tttggataac agaaccattt
    12781 tttaaatgtt gtaaatggtc atctaattta taagtatgtg ctatatgagt tacattttta
    12841 aatttttctt caaatgtaag agcaattttt tccatgtaat tgaagtcagt attaccattt
    12901 gtatttgttt ctggtaataa tattatattt tcccttatta caccaaggct attaagagaa
    12961 gctaaaaatt ctctatttct atattctgat ttttgtatat ttgttaggtt ttcatatttt
    13021 ttatatttat caaagacgga tataccacat ccagtagata ctaaaaccac aaatacatga
    13081 ttttttcctc tttcttttat agcattaaca atagcactcc cagcccacaa cacttcatca
    13141 tcttgatgct gaacatagaa gacaacataa tctttaaatt ttttatgact ataattcaca
    13201 tagctactaa aagcggttaa aatcaaacat aaaactagaa taatagatag aattttatat
    13261 ttatttatag taaatttttt attcttaaat gtcatttaat catctcctga aaggttgtct
    13321 tagttatatt atcaaacaaa gtactaaaaa ttatttctta aatgctgaaa aatatagagt
    13381 agattaatgg aataaaattt ttttattagt ttaaaataaa gaaaacattt atgtataaga
    13441 ctaatcaaaa tattaatgat tgataggaat tgagatttaa agggattgta catttattcg
    13501 ggattcattt taatagaatt ttactttagt acttagaata aaagtaataa ctttttaaat
    13561 ttaaagagta cacatatata tttgtgtact ccttgatgat atgattgctt atttactaca
    13621 aaaacagagt ctttttaaac ctaaaatatt tgatgcacca gctatcgtag ttccataata
    13681 gtaaactggt ttgttaagtg atagaaaatt tataattgag ttattacata atgtacttcc
    13741 tgtgcaaatc actaaatcag cccaagatat tacatcttca taatctcgta taccatcttc
    13801 aatcttaaca ttatattgaa tagtatccac aaattcagga tttaaatcta atactcttgt
    13861 ttcgaaaaag tccttaatat tatcaattat ggctggttga tatcctataa ttgctacttt
    13921 tggattacca aattccattt ttataaactc agggaatttt tttgcacata cctctggctc
    13981 gttattttta caatgaatgg ttctatccgt cttgcctaag tatttcataa ctgcatttaa
    14041 tgatgctata aaaagacttc tatcatgaga attgtcatgt aaatctaatg ataatatgtc
    14101 ttttaaagaa ccttcaaatg tagatggagt agaagtaaat gcttgtcctt tagctccttt
    14161 aaagtcagct tcaagcatta tttctttacc aactataatt gggaaatctt tacgcttagt
    14221 atttccaatt gcttcttttg aagatagaga tttggacaca atacttatca tatccatatt
    14281 taaattatat ttatcgactt gtactaatag ataatctctc aaaatatcaa atatattcat
    14341 agaaaaaaga ttctccttta ttttttattt cttcatttaa atcaaaaata tgatttattt
    14401 ttccattgtt cattattcct atcttatgag caaaaaacat tgcttcagaa atatcatgag
    14461 taacatgaat cactgttggt ttgtatttta tgtagacatc cttcaaaatt ttgcttatat
    14521 gtttttttgt aatataatcc aatgaactca taggttcatc taaaagtaat atatcaggtt
    14581 cagctattaa ggcacgtgca aaagcaactc gttgcttttc gcctccactt aaatttttag
    14641 gatacctatt ttttatatct tctatctgca ttaatttaga caagtggtta gccttaagaa
    14701 tctgttcatc tataggtatt tttctcaatt taagtggaaa ccttatatta tcttcaacat
    14761 tcatatttgg aaataattca aaattttgat atacaaatcc tatatttcta ttttctggag
    14821 gtaagtttga tattaaattt tctttgtatg atacatttcc tttagcagat ggatatcttc
    14881 ctgctaatgt ttctagaaat agagttttac ctgacccact agctcctaat aaaacaaagt
    14941 attcatgatt attaatattt aaactatttt cttgtaattt aaaattaggt aaatcaagag
    15001 ataaattttt tatttttaac atattaattc ttatcaacct ctctattaaa aaatatctca
    15061 aaaagcatca agcatgtaag tgagagaaat atcaaaatta cagctattcc gatagcaatg
    15121 tctatatcac cagtagacat atttaaatat atagatgtag gaataatttc agttttcata
    15181 cgtgtagaac ctgctatcat agctactgca ccaaattcac caagtgctct tgaccaagtc
    15241 attacagttg cagatatgat tccagttttt agatttggaa gaattataaa tctaaatgct
    15301 tcccaactat tacatccaag ggtacgtgca acaaatatca tcttttcatt actttcattt
    15361 aatgaagtgt gaagtatttt aatagtaagt ggaagattta caaatatttg tgctaaaact
    15421 ataccttgct ttgtaaatat aaaatcaagc ttaaaaatct tataaatgga atctccaatc
    15481 cccattcttc caaacaaaag taaaagagca attcctgata ctaagtgtgg aagagacata
    15541 gggagattta ttatctgtat aatcagtttt ttaaatggta atttagtaat atgtaaaaaa
    15601 tatgctactg gaatagctaa taaaaggcat actatagtag atattaaaga tgttttaata
    15661 ctaagtgtta ttgcatactg tacttccact gattttaagc atataggaac ataacttatc
    15721 cctttaatga ttaacataag aagtggtgat acaataaaca aaaagactat agcaaaaaat
    15781 ccagtaagta gagtagtaaa aaatttattt ttcaagttta atcactactt aacaggttca
    15841 aaaccatatt ttttaaatat ctgtttagct tcgtctgact ctatataatt tataaattta
    15901 ttagctaaat cttttttatc actatagttt acgattcctg ctgatatgag ttggtctata
    15961 tttttatctt gggcaatttc tataacttct acatcttcat taccaaagac cgaatctttt
    16021 gtggctattg tagcatcagc tttacttgat gttaactgta caatcatttc atttacagtt
    16081 gcagctgtag agacaacatt atcatttata gattttaaat tatttttttc aagtattttt
    16141 tgtgtagtct tacctatagc attagactct ttgtctccca aaataagttt aactccatct
    16201 ttatttaagt cttccaattt ttgaatatta gctggatttc ctttaggtac aactattgca
    16261 ggtgtatgat gtgcaaccgt tttatgttca cctacaagtt ctttttcaaa ggcctttgaa
    16321 taaattggtt cagaaccaac aataaatacg tctcctttgt gacttgtctc aatttgagct
    16381 attagctgtg cagaacctgc atagctatat tcaactttga cattattttc tttctcaaat
    16441 ttttttgcta tttcatccat tggtttcttt aaacctgccc ctgaatagac aaaaagagtc
    16501 tctttttctt ttgaatcatc agctgttgtc actttagtat tagattcatc attagctttg
    16561 ttttctttag aattacatgc agtaatactt aatgaaataa tcatagctaa agctagtact
    16621 gaaagttttt taaacataag atattccccc ttattaaatt agtgtaaatt cttttttagt
    16681 atatcatact aagaaaacga tgtctaagtt tttgttatgt tttataatgt tttgttatgt
    16741 tttgttatgt atagttaaga aaaaatctat tcataatgtc atttccatag gtagttattt
    16801 tatttttaat ttttattaat actttaaagt attttaaatt taaaaactta aatcaaatta
    16861 acttattgta gattttttac tttttaacta attttatatt tattatattt cagtgaaaaa
    16921 atagaacatt gaaacatata gatatgtttt aattgctttt tattaacaaa ttactaaaag
    16981 tattatatca gaattgaata caaaagaaag agtatgtatt tatgagatag ttgaaaattt
    17041 tgatgtgcca caactaaaat tattatatta ctatatattc taattaaaag agtacttgta
    17101 ggtttaagta catactttat aaatatattt ttgtcagatt tattttataa aaagtattta
    17161 gactaagtat tatttttaag tatttttttg ttgaagttat tttttccttt tttacaaaat
    17221 gcgtaaataa aaaaaagact cttaaaagag tctttccgaa aaatctatat attataatat
    17281 agttatattt tctgcttgag gacctctagc gcctttaact atatcaaagc ttactttttg
    17341 accttcttct aatgatttaa atcctgaagt ttgtatagct gagaaatgag caaatacgtc
    17401 atctccactt tccattgata taaatccaaa tcctttttca ttattaaacc attttactat
    17461 tccattattc attaaaaata ctcctattaa cctatatcaa gatatacttg atttacttgt
    17521 gtattaccac aaatactatt gtatcataga ttaacataaa tgtctattta attataattt
    17581 ttagttaaca tttttattta ttcttaatat gaaaataaat ttaaacattt tatattacca
    17641 atatattaat gtaaatgtag ataaaactaa aaagggtttt ctagaaaaaa atctgtctaa
    17701 ttatacttac ttaccaatac catcacttga tgcaagcaag attccacaac tttttctccg
    17761 tactctaata taaaatcatc taactgtgta tctataaaat cacaataatc atatgaacta
    17821 ccaatataag cagttaacat atgtgtacat ttatccattt tatctctaaa cataatgtat
    17881 ttatccccct aaaaaacttt catgaaaaac aaaaagaaaa tatctttttg gcaactggct
    17941 gacataactt attttataag cgttagtccc tatagctttg cgtcactaaa tttctctagt
    18001 tttgccgatt tagttttatt ctacagatta ataatacaat agaattagtt attattcaac
    18061 atatctagct gaaaaattaa taaatttata tatgaacgtt aaattatctg aaaaaataaa
    18121 aagcccctct gtcaggagtg acttaaaaat tgaatttaag tttgtattta aaacacattt
    18181 ttaataatgc aaaaactaat caaaaataaa caattcatca ataggtgtat ctactatttt
    18241 tgatatatct attgctaatt tcaaagaagg attatacttt cctgcttcta aacgcataat
    18301 ggtttctctt ctaactccta ctttttctgc taattgttct tgagttaatt cttttaattg
    18361 tctatatttt tttaggttac aagtgaattt aggcatatta tttttcctct aaaatataaa
    18421 gttttatagc ataaacattt aaagtgattg catatgtaat tgatagtaaa acacacatta
    18481 attctatatt cctagacact atagtaatac accataaaat gaataattct ataatagcaa
    18541 gctgtcctgt aaatgccata gctgcttttt catcttgtac atacctttca tctgctttat
    18601 caccatttat ttttgatata ataaagtttg agaaaaaagc aaaaaatcca atatatgtaa
    18661 ggtcagcaac attgccagat gaaaaatatc taagacttaa aaatccaagg aaaccacaaa
    18721 aacccataaa aaattttttc tgaatcattt tttatcctcc ttagtaattt taaaagagat
    18781 atatttatat ctttatgata caaatatatc tcttttatat tcttttgtca atagaaaatc
    18841 agtattatac aatatttgta ctgcaccata attgttagag caaaaatcta ctgcttgaga
    18901 tgtttatgta tgactaaata tgattttaat ttcaaattaa aagtagctaa attttatttg
    18961 aatagagaag gaagagatat tttaatagta aaacaatatg gagtttataa ccattctcaa
    19021 gttgaaagat gggttaatat attaggagag tgtgcactaa aaataaaaaa aattttctat
    19081 actatatggt tcaagttaga tgttataaac tatatgatga taactaaata tagtgtacaa
    19141 taaatcgcta agtattttgg aataaacaat agcacagtta tagcaacatg aaaataaaaa
    19201 tttttacata gtggtgttga tgccttttct aaaatgaaag gaagaccata atgaataaaa
    19261 acaaaaacac taaagaaaaa ataaattaac aagagaacaa gaattagaat atgaaaataa
    19321 atttttaaaa gatgagcttg agtttttaaa aaagatgtgt gcctcaggga agccacatta
    19381 aaaaacagtg gtatcattat taatcaaaag aaagttagat gtattatcaa aaaattagaa
    19441 cttaaatgca tagcattttt tcataaatca cataaatata actcctataa atgtataatt
    19501 ggaaaagtaa aaaataataa aacaaataga agatttaaaa tatcaatagc atatcagaaa
    19561 ataacaaatg atatgctaga atttataaaa tatatctaat gataaaaatg gaaaatcaat
    19621 tataaaaaag gcatatcccg attcattttt tgttatatag tcaaataaga atactataga
    19681 tgaaagatac gattttgcat atgtattaag taagtattct gatgtttttg gttgatacac
    19741 atgcaataaa gaagttgtgt ttgcagataa attagcaaaa ctatagtata agttgcaaca
    19801 ataaagaaaa tgaaaattat catactaaag cataaggcat gttacaattt tattagatag
    19861 gacattaaca tacattaaag tactttttat tataaagagt acttattttt ttgaaattca
    19921 ttaatgtata aactatcaag aatattactc aacataattt aaaaacaact tgaataggta
    19981 tttttaaatt atgtgatata ataaaaaata gaagtgtatg ccaatacact tctgtaatta
    20041 taactaagca ttctattctt gaggagtgct tttttcattt ctccaaacaa gattgtttat
    20101 ttcatcagca attattaaat aaaatttatt gtatttaagt attcgattta tcatttaaaa
    20161 attttattac aatatctatt ttgttttgct cgttcattat gtctattgaa cttccataat
    20221 cactttttaa tttatctaga ccacttttgc ttatttctcc ttctggaagt atacttaatt
    20281 ctagatattt ttcatgacca ggagatgaca ttaatagata tgaaccatta taaggcatac
    20341 ctaaattttc tattttatct gctaatgggt catctttaaa gccaccattt attattattt
    20401 ttccatcttt ctttacccat gatatcattc ctccatcagt atattttgat tcatctgcat
    20461 agtagctaag agacgcactt tttattgcac ttatatcagc ttcaagttta gctatctttg
    20521 ctttttctat atttttaaat aatgctggaa gtgctactat tgccaatatt cctattatag
    20581 caattactac taataattcc actagagtaa atcctttttt atttttcatt tatttcccct
    20641 ccatataaaa ttaataatat ataaattttt attttgttga tttctacttt ctatatttta
    20701 ccatatatag acaaaaaaat cactttctat gacaaatata ctattatttt acaatttatg
    20761 gaaatgattt aaaatgacat ttcttgttag aaaagatata agaaatggat taaaataaag
    20821 aaaactacag tcatatcaat gtcaataata ttatttaaaa gcagatgaaa ataaaaagag
    20881 gactcagaaa ataatgtcta tttaattata cttacttacc aataccataa cttgatataa
    20941 acaagattat actacatttt ttttatgctc tagtataaaa tcatctaact gtgtatctat
    21001 aaaatcaaaa taattatatg aactatcagt ataataagtc aaaatatgta tacatgaata
    21061 catattttaa aagatatata ttattacata cctaaaaaac tttcataaaa acaaaaataa
    21121 aatatgcttt tagtaacttg ttaacataac ttataattta ttttataggc attagcctat
    21181 atagctttgt gctactaaat ttctttgatt ctagcgattt agtgctattc tacaactaaa
    21241 ataatacaat agagctattt attgtttggt agcattgttt gcaaattaaa caaatttata
    21301 tatatgaata ttattttacg tttaaaaaaa cagactaaat atgtagaata tttggtaaaa
    21361 cttacctcta gtttttgtct taaaaagctc ctattattaa tttaagttag aagaattaag
    21421 gaagtgacat aaattgaaag aagaaatatt aatagaaaaa ttattaagag tagaattaat
    21481 agtaatggag tatttatgga aaaaagacac tctaatgcct aaaaaaaaaa ttgtaaaaga
    21541 aataaaacag atttatggat ggcataaaag tacaataaaa atactactaa aaaggctagt
    21601 tgataaaagg tatttggcta gatatattat aaattttcaa tcttattata aaataataat
    21661 agataataaa gaatattata cttttaagaa aaaggtacta aaatctagta aaagcataaa
    21721 aataatgcat tcacttacca ctatacataa aaatgtaagt aaagagaagt tagacctatt
    21781 agatgagtat tatagaaact taaaagaatg atatagtaaa aatcgaatgt aattatttac
    21841 cttaattcct ttgtttgtaa aactttattg atatagaatt tgacatatac ataaattggt
    21901 aaagggatgt atactatgaa aattagtaaa ttaccagaag cggaattaaa agttatgaga
    21961 tatatttggg aatcagaaaa ggtattaata ccaagagaaa tcaccaagtc tatggaacaa
    22021 aaatatgaat ggaaagatac aactatattt acagtattaa aaagattact aaagaagaga
    22081 gtttttagaa gcggagaaaa tagataagtg tacacactat aatgtattag taaaaaaaaa
    22141 taatatcaga gattttaaac aaaagaattt ttaaagaata cacataaaaa ttcaatatta
    22201 agtttaatgt cagcactatg tagtaaggat gatgaagtag atggtgatat tttaaattta
    22261 ttagaagaat attttaagaa tttgaaataa gaaaatgaca aat
//
LOCUS       DBGJFU010000061        22201 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.061, whole genome shotgun sequence.
ACCESSION   DBGJFU010000061 DBGJFU010000000
VERSION     DBGJFU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.061"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            364..3018
                     /gene="adhE"
                     /locus_tag="ACOIAS_002154"
                     /gene_synonym="adhC"
     CDS             364..3018
                     /gene="adhE"
                     /locus_tag="ACOIAS_002154"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGZ2701862.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            3294..5513
                     /locus_tag="ACOIAS_002155"
     CDS             3294..5513
                     /locus_tag="ACOIAS_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2701863.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            5767..6429
                     /locus_tag="ACOIAS_002156"
     CDS             5767..6429
                     /locus_tag="ACOIAS_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HGZ2701864.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            6800..8209
                     /locus_tag="ACOIAS_002157"
     CDS             6800..8209
                     /locus_tag="ACOIAS_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HGZ2701865.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            8324..8599
                     /locus_tag="ACOIAS_002158"
     CDS             8324..8599
                     /locus_tag="ACOIAS_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HGZ2701866.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            9229..9540
                     /locus_tag="ACOIAS_002159"
     CDS             9229..9540
                     /locus_tag="ACOIAS_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701867.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            9542..11467
                     /locus_tag="ACOIAS_002160"
     CDS             9542..11467
                     /locus_tag="ACOIAS_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HGZ2701868.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            11470..11961
                     /locus_tag="ACOIAS_002161"
     CDS             11470..11961
                     /locus_tag="ACOIAS_002161"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HGZ2701869.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            12013..12576
                     /locus_tag="ACOIAS_002162"
     CDS             12013..12576
                     /locus_tag="ACOIAS_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HGZ2701870.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            12591..13568
                     /locus_tag="ACOIAS_002163"
     CDS             12591..13568
                     /locus_tag="ACOIAS_002163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HGZ2701871.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            13561..13878
                     /locus_tag="ACOIAS_002164"
     CDS             13561..13878
                     /locus_tag="ACOIAS_002164"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HGZ2701872.1"
                     /translation="MYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVEDEY
                     AIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGADIL
                     FKE"
     gene            13995..15773
                     /locus_tag="ACOIAS_002165"
     CDS             13995..15773
                     /locus_tag="ACOIAS_002165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HGZ2701873.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            15770..17143
                     /locus_tag="ACOIAS_002166"
     CDS             15770..17143
                     /locus_tag="ACOIAS_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HGZ2701874.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            17149..17817
                     /locus_tag="ACOIAS_002167"
     CDS             17149..17817
                     /locus_tag="ACOIAS_002167"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HGZ2701875.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            18094..19143
                     /locus_tag="ACOIAS_002168"
     CDS             18094..19143
                     /locus_tag="ACOIAS_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2701876.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            19239..20228
                     /locus_tag="ACOIAS_002169"
     CDS             19239..20228
                     /locus_tag="ACOIAS_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701877.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            20233..21951
                     /locus_tag="ACOIAS_002170"
     CDS             20233..21951
                     /locus_tag="ACOIAS_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HGZ2701878.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
BASE COUNT         8573 a         2601 c         4081 g         6946 t
ORIGIN      
        1 ctttaacttg ttaattaagt tttaattttc attttcaagg atttttattt ttaaacttga
       61 tagagtatca tattaaaaat tctttttata aagttttatt tgcaaaaaat acaaaaatat
      121 aaacgttaaa aaaataacgt tatccttaag tttttagatg atttcattag tgatttgcgt
      181 aaaaaataaa gtgatatcta gttaaattaa gtatttgtaa agctagatat tattagtaga
      241 aatgatgtta tattagataa aaaataaaat gttaaaaaaa taacttgaaa ataggaaaag
      301 taaatgctat aatgttaata aattaacata ataaataaaa aactatctca ggaggttgtt
      361 aaaatggctc ataaagaaca agtaataata gaaaataaaa aagaaaatga agttatgtca
      421 gttggtttag atactgtaaa taacgttgaa agcttagtta aaaaactagc aaaaatcaga
      481 gaagctcaaa aaatatttgc tacatatagt caagagcagg tagataaaat ttttttagca
      541 gcatctttgg cagctaataa gcaaagagtt cctctagcta taatggcaca aaaagaaact
      601 ggtatgggaa ttgctgaaga taaggttata aagaatcatt atgcctcaga gtatatatat
      661 aatgcatata aagaaactaa aacttgtggt gttatagaga aagacgaagc atttggtatg
      721 actaaaattg ctgaaccaat aggtgttatt gcagcagttg tacctacaac taatccaact
      781 tctactgcta tctttaaagc acttttagct ttaaaaacta gaaatggaat aattttctct
      841 ccacatccaa gagcaaaaaa ttctactata gaggctgcaa aagttgttct tgaggctgct
      901 gttttagctg gagctcctga aggtataata ggatggatag atgagccatc tttagaatta
      961 actacaactg ttatgaaaga agtggatttg acccttgcta caggaggtcc tggaatggtt
     1021 aaatcagcat attcatctgg aaaaccagct attggagttg gtgcaggtaa tactccagca
     1081 ataattgatg atagtgcaga tataaaaact gctgtaaact caattcttgt atctaagact
     1141 tttgacaatg gtatgatatg tgcttctgaa caatcggtta tagtattaga aaatatatac
     1201 aatgaagtca aaaaagaatt taaagagcgt ggtgcttatt tattagataa agatgaaaca
     1261 gaaaaaataa gaaatataat tttagtaaat ggaggattaa attctaaaat agttgggcaa
     1321 acagcttgta ctatagcaaa acttgcaggc tttgaagtcc cagtagatac taaggtttta
     1381 ataggtgaag tagaatcagt tgaaatagaa gaagcttttg ctcatgagaa actttcacca
     1441 gtacttgcaa tgtacaaggc aagtgatttt gatgatgcag ttagaaaagc agaaaaatta
     1501 gtagaagatg gtggatttgg gcatacatct tcattatata ttgatgatgt aaatcaaaga
     1561 gaaaaacttg ataagtttac ttcagccatg aaaacttgta gaattttaat taatactcct
     1621 tcttctcaag gtggtatagg ggacttatat aattttaaac tagctccttc tcttacttta
     1681 ggatgtgggt cttggggtgg aaattcagta tctgaaaatg taggagttaa gcatttactt
     1741 aatattaaaa cagtagctga gaggagagaa aatatgcttt ggtttagagc accagaaaaa
     1801 gtttatttca aaaaaggttg tttaggagta gcattaaaag aacttaaaga tgtaatgaat
     1861 aagaagagag cttttattgt aacagataca ttcctttaca ataatggata tactaaagca
     1921 gttactgatt tattagatga aatgaatatt aagtacacta cattctttga agtagaacca
     1981 gacccaactt tggaatgtgc taagataggc gctaaagcta tgagagaatt taatccagat
     2041 gtaataatat ctataggtgg aggaagtgca atggatgcag gaaagataat gtggacattg
     2101 tatgagcatc cagatgtaga tttccaagac ctagctatga gatttatgga tataagaaaa
     2161 agggtatata cattccctaa aatgggtgaa aaagctaatt ttgtagctat accaacatca
     2221 gcaggtactg gttcagaagt aacaccattt gctgttataa ctgaccaaga tacaggagta
     2281 aaatatccat tagcagatta tgaactgatg ccaaatatgg ctatagttga ttctgatatg
     2341 atgatgaata tgccaaaaag cttaacatct gcttctggta tagatgcttt aactcatgca
     2401 ttagaagcat atgtatctat gcttgcaacc gattatacaa atggattagc tttacaagct
     2461 attaagagta tatttgaata tcttccacgt gcatatgata atggtgcaaa agacccagaa
     2521 gctagagaaa aaatggcaaa tgcttctaca atggcaggta tggcttttgc aaatgcattt
     2581 ttgggtgttt gtcactctat ggctcataaa ttaggtgctt tccaccatgt accacatggt
     2641 gttgcaaatg ctcttttgat aacagaagtt atgaaattta attcttcaga tgcaccaaag
     2701 aaaatgggag cattctctca atataaatat ccagaagcat taaagagata tgctgaaata
     2761 gcttcatttt taggattaaa aggaaattct gatgaagaga aattccaaag tttattggta
     2821 gctattgaag atttaaaact taaagttggt attcctaaat ctataaaaga gtttggcgtt
     2881 gaagaatcta agtttatgga ttcaatagat gaaatggtaa ttcaagcatt tgatgaccaa
     2941 tgtacaggag caaatccaag atatccactt atgaatgaaa taaaagatat gtatttaaat
     3001 tcatattatg gaagataagt ttgctataaa atagataaaa taaaaactct tatacataaa
     3061 acaaagtttt attgtataag agtttttttg tttgagaaaa tggatagttt ttattcttta
     3121 atttaaaatt caattaaata gtgttattat aataataaaa gaatgttttt atggttatta
     3181 tgttgatttt atgtgtaaca tttaagaata gttatataca atataaatga tgaattggta
     3241 taaaaaggat atattatcta tatgaagtat tgactaatta ggggaggagt cgaatgacag
     3301 ggagaaataa aagttttaca ttaattacaa ttcttttttt aacatcatta tttttgacaa
     3361 tggggtatat ctatatagaa gatacaaaac atcttcttat gtcagaggca aaaatacata
     3421 taaaagaagt tgcagtacaa ggctctcaac tagcacaaag acaaattgaa aaagatttag
     3481 atgttttaaa tattttttct aactattatg cttcaaatcc agatatacct aatgaggaaa
     3541 aaatgaaaaa tcttttagat gaaatggaaa atcaaaaatt ttacactatg gcaatagttg
     3601 atataaatgg taatgctgaa aataccaaag gtgataagtt ttcagtgaaa gatagagaat
     3661 ttttcaaaaa ctcaattaaa ggtaagaagt atgtttcaag tccatatgtt gatgaagtaa
     3721 ataagtctat aaagaagatt gctatttcag taccattatt aaataatgat aaagtagttg
     3781 gagttttata ttgtacttac aatatcaata ctctgatgaa acttattaat atatcttttt
     3841 atgaaaataa tagtatatct tatgttgtaa aaaatgatgg tacaattatc cttcatccac
     3901 aaggggatag cctatcaaaa aatatttata aattgttaga acaggataat gatattcaag
     3961 aagtaaatag attaaaaaaa gaattgcaag aaaataaaac aggtgcaact gtattaaata
     4021 tgttggaaga aagaagatat ttagggtatg ccactatgga taatgggaat agtgaaaata
     4081 attatataaa agactggaat ttaatttttt caattccaga aactgttgta tttagtaatt
     4141 caaaacaaat aatcaacaga gctgtatatg cagtgttatc tattgtatta atatttgttg
     4201 caattatttt ttatataatt tatataaaaa aatctaatga aaaagaaata ttaagtttgg
     4261 cttatgaaga taaagttaca tatataggaa atcaaaataa attttataga gaatgtagta
     4321 agtatctttt agataaacca tctttaaatt atattatagt atattttgat attaataact
     4381 ttaagatgat taatgatact tttggatatg aatttggtga caatttatta attacaatag
     4441 caaaagcttt aaaagaagaa ttaacagaag gtgaggtata tgcacgactt tcaagtgatt
     4501 attttgcaat tttctgtgat tataaaaatg gaagaaataa aattataaga aaacttgata
     4561 atatacgaag taatattgaa agtaatttga gtatagtctt tgaaatttct ctgtgtgtgg
     4621 gtatttattt tgttgaagaa ggtgaagtag atattcaaaa ggctgtaaat aaagcaaata
     4681 tggctaggtc tgttgctaaa ggtaaaaata taaattatgc tatttataat gaagatgtta
     4741 ggaataagct tagtgaggaa tcaatgattt tagacgatat aaaaatagca ttggtaaaaa
     4801 atcaatttga agtgtattat caacctaagt tttcacttgt aactggagaa atgataggga
     4861 gtgaagcact aataagatgg aatcatccag aacatggttt tataagtccc gcagtattta
     4921 ttccaatagc agagaaaagt aagttgattt taaaaattgg gagatttgta tttgaaaggg
     4981 tttgcaatga tttatatgaa tggaaaaagc aaggtaaaaa gatagttcca gtttcagtaa
     5041 atttatctag ggtcgagttg tatcaaccag atattgtaaa atttatcaat aaaacaataa
     5101 aaatgtataa tttaagttca gatttcattg aaatagagat aacagaaact gtggctatta
     5161 atgagttaaa tattttaaag aatgttttaa atgagttgag gacgtatgga ttttctattt
     5221 ctatggatga ctttggtaca ggatattctt ctattagttg tttaagagat atgccaattg
     5281 atatattgaa gctagataag tcttttcttg gtggcattga acatgatgag agaagtcgca
     5341 atatagcaaa atcaatagtg tctctagcaa aatcactaga tttagttgta attatagaag
     5401 gtgttgagag taaagaacaa gctgagttaa tgaaacaatt tggatgtgat ttagttcaag
     5461 gattttattt tgcacgacca atgccagcta agaatttttt agatttactt taaaacaaaa
     5521 tttgattttt atataaagca ctattatgaa tgcttgattt taataaatca ttgctaaata
     5581 attagtgttt ttttatttta tcaatgtatt gtctaatata aagcaaaaaa taattgtatt
     5641 ttataaaaat tatattaaaa ataaatttat tttgctaata aacatgttat taacaaaaga
     5701 atagtactat aatgtataga tgataaaaac tttaataaaa atttagtgta taggagttgt
     5761 ttaagtatga tagatataag tattgatgac aaacttaagg aaagatggcc taatgctaag
     5821 ctgggttgca ttcaagcaaa agtagcagta aataaaagct cagaaaaatt aattaatgaa
     5881 ataaatgagt tttgtgatac tataaatcaa agtctaaaag ttgaagacat tacgaaacta
     5941 gataaaatta aagatgcaag aaatgcttat aaagagcttg gaaaaaatcc aagtagatat
     6001 agaacatctt cagaagcatt ggttagaaga attattcaag ggaagggtct ttatacaata
     6061 aataatatag tagaaataaa caatttaata tcaataaaat cattatatcc agtaggtaca
     6121 tataatgtaa gcaaattaca ttcaccagta tgttttactg taggagatga aggagaacag
     6181 tataagggta ttgggaaaga attaataaat atagaaaatc ttcctatatt gtctgattca
     6241 gtgggtaaat ttgggagtcc aactagtgat tctgaaagag caatgataac agaagatgca
     6301 aatgaaatat taatttgtat attttctttt agtcaagaat ctgatattga agaatatttt
     6361 ggatatgcta aggaattact taaagaatat gcagatggaa aagatataga aactaaaata
     6421 attaaataga atctatataa caacgttatg ttaagctaaa attcaatata aaaataagtt
     6481 aagataaaga aactaacaca aaattagttt cttttttaaa atagtagttt agtgttacaa
     6541 aaatagatag atagtcccac gattattaaa agtatggtaa tatattaata tagcagagat
     6601 tgtgtttaag ggattttgaa aggggggatt tataataatc attttatcta aaagataaaa
     6661 tggttttata gaaataacta tatatttaaa aatataaata atgatacagt aatttgggta
     6721 tataaaataa ggcatacatg gttttgttta aaaaccaata aggtacatag gtaattacaa
     6781 tgtaaggaag gatttaatta tgattgatgg aaaagaggta aaacaagaaa gtggacaaaa
     6841 aaatctaaaa tttaagatag ctatagtagt aggtgttttg tttgtagctt atataggaat
     6901 ggcaatcttt tttagaaatc atttttactt tggaacgact attaatggcg taaaggcttc
     6961 aggaaaaaca gttgagcaag taaatgattt gttagcatct agtactaatt cctatacttt
     7021 aaacttagag gaaagaaaca ataagaaaga acaaataaaa gcaaaagata ttgatttaaa
     7081 atttacacca gatgataaag ttcaaaagat aaaagatagt cagagcgcta ttggatggat
     7141 tggtggaata tttaaatcta aagagtataa taatatgata actctttcat ataataaaga
     7201 tttactagaa aaaaggttta atgctttatc ttgttttgat agtaaaaatg ttgtagaacc
     7261 taagagtgct tatcctaagt atgatagcac taaaaacgct tatgttatag taccagaggt
     7321 acttggaaac aaagtgaaaa aagatgattt ttataagtta gtacaaaact caattaattc
     7381 tgggaatact tctatttctc tggatgaaag taaagtttat gaagcaccaa acaacacaaa
     7441 ggattcagaa aagttgaaac aagctattaa gacattagat aagtatgttg gtgtagtcgt
     7501 tacatatgat tttagtgacc gtaaggaaac tttagatggt tctgaaatta ataaatggtt
     7561 aaaagtagac tcagaaaaga attatgacat aacttttgat aatgatgcta tggtagaata
     7621 tacaagaggt ttaagtaaaa aatacagtac ttttggtgac tcaagaccat ttaaaactgc
     7681 cacaggtaaa gatattacaa tttcaggtgg aatttatggt tggttgatag ataaaaagaa
     7741 agaagctgaa gcattagttg atgttgtaaa agctggtaaa aatgttacaa gagaacctat
     7801 ttacatacaa aaagctgtat ctcgtaataa agatgatatt ggaaatacat atgtagagat
     7861 tagtctttct ggccaacata tgtggttctt taaaaatgga gaaatgcttg tatcaacaga
     7921 cgttgtaaca ggtgatttag ctagaggatt tgctacacca tcaggtatat atcctcttaa
     7981 ttataaggct agaaatgttt cattaactgg tcaaggttac agttcacctg taagttattg
     8041 gcttccattt aatgggaaca taggcattca tgatgctact tggagaaatt cttttggtgg
     8101 aagtatatat aagtcttcag gttcacatgg atgtgtaaat acaccttttt ctaaggcaaa
     8161 gaaaatatat gaaaatatag aaccaggtac accaattatt ttatactagt aaatgtgttg
     8221 ctaacagtta agcgaagtca tcctagttta tttaggatgg cttccttttt atataaagct
     8281 tattgaagtt tacagaaaga ctacttaaaa ggagaaatac ataatgaatg aaaaattaac
     8341 taaaatttta gaagaagcta agaatgatga tgaatttaga aaggaactta taaaaacaag
     8401 agaacaacgt gatgttatgg attcattttg taaggttgtt acatctagag gttatgatat
     8461 gacagtaggg gaactttttt ctatgggaga agaatataca tccaatctac tgaaaagtgt
     8521 aaatggagga gcagcatatc ctattgaaga ttggagtgat tggtacgagg atttttttat
     8581 aatgttgtcg aatatataga gtaactaatg atattatcta cttaagatta atacattgcc
     8641 ttaaaataaa actattagaa ctaggtgtaa gttttatcaa tctttaaaaa tattgattta
     8701 tttctaacta gatataaaaa aattagatta aaaataaggt gacaaaaatc gatatagctt
     8761 gatgatttat aaaagttaga taaaatagtg cgagatattt ctaaaaattc aaaaaaaaca
     8821 ataaacttca atggttagag gttgaaaaag aatataaaaa acttttatat gtaataaatt
     8881 aaattaaaat tataaaaaaa ataaaataaa aatttaaaat gtcgacataa cgttaatttt
     8941 aaaacaaaat aacgctattg acaaactata tcgctttaaa ttataattat tgaaaataat
     9001 ggtatttaaa aaccatactc atgtcatagt ctaaaagtaa gtcaaaaaag gatgtaaatc
     9061 taggcaagta ctattctaaa atatgtttta ttaagaaata aacgttgaaa aatattaatt
     9121 attatataaa aatgctgttt atactttaac aatgaggtag taatctaatt agaataaaaa
     9181 aatctaattg gttatataca tagtaaaaag gtaagagagg tgatttggat ggcttttgaa
     9241 ataataaaaa gcattgttga ggcagagcag acagcagaca gtatcaaagt aaaagctgtt
     9301 actgatgcag agtctatcag agctgatgct gtaaacaaat gtgaaagcat atttgctgat
     9361 gtaaaaaaac aagcaaagct tatggaagaa actcttattg agaaggcagt caccgacagt
     9421 agagcagagg ttgataaaat cttagctaat gctaaaagtg aatgtctgaa aattgaaaaa
     9481 actgctgaag aaagaaaaag taaggctatt gaagcggtta ttggaaaggt agtgagataa
     9541 tatggcaata gttaatatga acaaaatatc tattgttggc ttagaagctc aaaaaagcca
     9601 gattttaaag ctacttatga accgtgggtt tgttcagatt gatgatagtg cctttcttac
     9661 tgaggaggag gaattaaaaa attatcttgt aaaagatagt gaagaatctg aagttataat
     9721 gctagagcag aaaatgtctc atgtaaacca agcaattaat attgtttctt cactagtgaa
     9781 acaaaaaaaa tccctacttg caccaaaaag agagtttaac aaaattgata gtgaaaaaga
     9841 agggcaagtt tatgctgaag ctctggagtt aaacaatttg cacaaggaga taggtacaat
     9901 aaggtctaac ataaacttac ttaacaacaa taaaagtatg ttagaaccat gggtgaactt
     9961 tgatattcct attgaaaata tggaaactaa gtatacaaaa actatacttg gaactgtgcc
    10021 cataagcgca aacattgatg ctttaagagt aaaattagaa gatgaaatac cagaaagtgt
    10081 tattgggatt gtcaattctg acaagttaat gaactatgtt tatttaatta ctttaaaaga
    10141 aacttttgat gatgttttag aagttttaaa agaatttaat ttttcagtgg tttcattacc
    10201 aaatgaagaa ggtacaccgt tacaagctat caagaaatac aatgaaatga taattagaca
    10261 ggaaaacgtt tctaaggaca aaacagagga aattaaaaga catgcagata gtatacacat
    10321 gcttgaaaac ctatatgatt tttacactat agaaagagac caaaaaaaga ttatagagag
    10381 acttgtcaaa acaaaaacaa cattctgtct taatggttgg ttaccaagca aaagggcaga
    10441 tgaattaatt aaagaaatta cagaaaagtt tgattgttgt gttcagactg aagaaggaag
    10501 taaagaagaa ggcttcccaa ttctgcttga aaacaataaa ttagtaacac cttttgaaag
    10561 tgtaactaac atgtacagct atccaagtac aaaagatatt gacccaaata caattttaac
    10621 tttcttcttt gttatattct ttggaatgat gctaagtgac gccgcatatg ggttaataat
    10681 tgcagtagtt tgtggatttg ttgtatataa attaaagata caaaaaggtg aaggaaattt
    10741 aattaaactt attggtatct gtggagtttc aactactgta tggggtctta tattcggggg
    10801 tattttgggg gatttaattc ctataaaagc attaattaat cctttagagg atgttatgac
    10861 acttatggga atgtcgttac tgttcggtat tatccatata tatgtaggtt tgggaataaa
    10921 agcatataca ttaataagaa gtggaaaggt aatagatgca atctttgaca taggtttttg
    10981 gtatttgtgt ataacaggtg tatgtctact tataatacct tttgttgcag gggacatagg
    11041 tgtattttca gaagtaggaa aatatttagc aattataggt gctataggtc ttgtacttac
    11101 tcaaggaaga agctttaaag gtattcctgt taagcttttt aaaggatttt caagtctcta
    11161 tggtataaca agttattttg ctgatatatt atcttataca agaattatgg cgctttgttt
    11221 aacaacagga gttatagcac aagttatcaa ccttttaggg gctatagctg gtccgatact
    11281 tgcagttgta attggtgttg taggacacac aataaacctt cttataaatg cactaggtgc
    11341 atatgttcat acaagtaggt tacagtatgt agaatttttc aataagtttt atgaaggcgg
    11401 aggagttcct tttgttccgt ttaagtataa aacaaaatat acaagcatta ataaaaagga
    11461 gatgtaaata tggaattttt aaacgcgtta ggaaatggtc aatttttggc aatatcagga
    11521 gctgctatag cagcattatt tgcaggtatg ggttctgcta aaggtacttc aatagcaggt
    11581 caagccgcag caggtgttgt aacagaagac cctagtaaat tcggtcaact tctagtttta
    11641 caacttcttc ctggtacaca aggtctgtat ggatttatca tagctttcct tattttatca
    11701 aaagcaggga ttattggtgg agatgcaata ccaacatcag ctcaaggttt acaacttctt
    11761 atggcaggac ttccaattgg tttagttggt ttagtatcag gtatagcaca aggtaaagca
    11821 gcagcagcag gagttggaat agttgcaaaa agacctgaag aacttggtaa agctataaca
    11881 tttgcagcaa tagttgaaac atatgctctt ttaggactac ttgtttcttt cttagcttac
    11941 aatggaattg caattggtta attataaatt ataattcata agtaaaaaaa atgaaaagga
    12001 ggaagcactc ctatgggtaa tgaacaaaaa atgattgaca gaattatagc tgatgccaaa
    12061 caagaagcac aagaaatatt ggataaagct aaaagtgaag cagatttaaa agtaaattca
    12121 gctaatgaaa aagctgaaaa ggaaatggct tcctatacaa agcttgcaga ggcagaagca
    12181 gaaaaagcgg cttctaagga aatatcagga gcttatatgg aggctaaaaa gcaaatatta
    12241 tcaaaaaaac aagagatttt agaagaggta attttagaag ctaaaaacaa acttttaaat
    12301 cttaaagata atgaatatga agagattata ctaaacatga ttgaaaaatc aaattgtaca
    12361 gatgattcag agatagtatt atctaaaaaa gataaaaaaa cattaaaaga tgtgctatca
    12421 aaaaaaggca ttaaagtgtc tgatgaaact agagatatta ctggaggttt catagttaaa
    12481 aaaggtgaca ttgagtacaa ttattcattt gaagccataa ttgcagtaga gcatgaatat
    12541 attgaacaaa ttgcagcaga aattttgttt aattaaggag gggttaaaaa atggcagaag
    12601 aaaaaactta tccctatgcc gttgcacgaa taagagtgct tgaaaaacag cttcttaaca
    12661 gacaaatgtt tattcaaatg gcagaagcca aaagccctga agactcttta agaattatag
    12721 ctgaggcagg ttatgctgat acatcaaata ataatataca taattttgag aatattttga
    12781 ctcaagaatt atcaaagaca tatgaaattc ttaaaagtct agctcctgaa gaaaaatttg
    12841 ttgatgtatt tctttataaa aatgactatc acaatttaaa agtgctgatt aaagaagaaa
    12901 tttctggtgt agatggtgag aaatatttaa ttgatggagg tacaattcct cttattaagc
    12961 ttagagaatc tttagcaaat cgtagtttta gtgatttacc taagataatg tcatcagcta
    13021 tattagaggc atttgatgtt tataataaga cacaaaatgg ccagatggtt gatattgttc
    13081 tagataaagc aacctttacc agtatgaaag aaactgctaa agaaagccaa aacaaatttg
    13141 taatagatta tgttatgaaa gtttgtgatt taacaaatct aaagagtttt ataagagtca
    13201 aaaatatgaa gaaagatttt gatatgttta tgaatgtgtt tgtttcaggt ggctcacttg
    13261 ataaggaaaa attcttagaa gctttttcct cagatactcc agcatcatgt tttaaatcta
    13321 cttcttactc tgatgtctgt aaaaatggca tggatagtgg ttttactgtc tttgaaaaac
    13381 tttgtgatga ctatcttatg gaatatgtta gaggagcaaa atttaagcct cttacattag
    13441 aaccactaat tgcatattta tatgctaaag aatcagagat aaaaacaatt agaattattt
    13501 tgactagtaa gcttaataat atagacgcag atactataaa agaaaggcta agggatgctt
    13561 atgtataagg taggagtagt tggagataaa gatagcatca tgggattttt agctcttgga
    13621 atagatatct tcccagccta tgatagtgac gaaattaaaa aaagtataca taagttggtt
    13681 gaagatgaat atgcgataat ttatattaca gaacaggcat cattgcttgc aaaagaatca
    13741 atagcaaaat acaaagatta ccaacttcct gccattatag ttattccagg tattggagga
    13801 agtatgggat taggtatgaa tgaagtaaga gaatctagta aacgtgctat aggtgcagat
    13861 atattattta aagaatagat gaaagttgaa taaaagacaa tctcaaattt tattggtgct
    13921 aaatatatgt gctaataaaa tataggaggg aatatttaaa ttttgataaa gtatatgatt
    13981 gcaggtgaga gaatatgaaa acaggcacaa ttgtaaaggt gtcaggaccg cttgttgtag
    14041 cggagggcat gagagatgcg aatatgtttg acgtcgtaag agtttcagat aaacatctta
    14101 taggcgagat tatagaaatg catggagata aagcttctat acaggtttat gaggaaactt
    14161 ccggtctagg accaggggaa gaagtagttt ctcttggaat gcctatgagt gttgaacttg
    14221 gaccaggtct tatttcaact atttatgatg gtattcaacg tccacttgaa aaaatgtatg
    14281 atatttcagg tactaatata actaaaggtg ttgaagtatc ttcacttgat agaacaacta
    14341 agtgggaatt taaacctaaa aaatctgttg gtgataaggt tgtagcagga gatataattg
    14401 gtggcgttca agaaacagca gtagtagaat gtaaaattat ggttccttat ggagttaagg
    14461 gtactataaa agaaatatat tctggtgaat ttactgttga agatacagtt tgtgttatta
    14521 cagatgaaaa aggtaatgat atacctgtaa ctatgatgca aaaatggcca gttagaaaag
    14581 aaagaccata tagagaaaag aaaacaccag attcacttct tgttacaggt caaagagtta
    14641 tagatacatt tttcccaatc acaaaaggtg gggtagcagc tataccggga ccttttggaa
    14701 gtggaaaaac tgttacacag catcagcttg ctaaatgggc agatgcagat atagttgtat
    14761 atataggctg tggcgagcgt ggaaatgaaa tgacagatgt tcttcttgaa tttcctgaat
    14821 taaaagaccc aagaacaggc gagtcactta tgcaaagaac tgtgcttata gcaaacacat
    14881 cagatatgcc ggttgctgca cgtgaagctt ctatatacac tggtattaca atagctgaat
    14941 attttagaga tatgggatat agtgttgcac ttatggcaga ctctacatca agatgggctg
    15001 aggctcttag agagatgagt ggtcgtttag aggagatgcc tggtgaagaa ggttatcctg
    15061 catacttagg ttcacgtctt gctcaattct atgagagagc aggaaaggta aattgtctag
    15121 gtatggatga aagagaagga acacttacag caattggtgc agtttctcct cctggtggtg
    15181 atacatcaga acctgtcagc caagcaacgc ttcgtatagt aaaagtattc tggggtcttg
    15241 atgcatcttt agcatataaa cgtcatttcc cagcaattaa ctggttgact agttattcac
    15301 tctatcagga aaaagttgat gtatgggcag ataaaaatgt aaatgataat ttctcagctc
    15361 aaagagctca agctatgaaa cttttacaag ttgaatctga acttcaagaa atagttcaat
    15421 tggttggggt tgattcactt tcagatggag atagattgat actagaggtt actcgttcta
    15481 taagagaaga cttccttcat caaaactctt tccatgaagt tgatacttat acatcacttc
    15541 ataaacaata tagaatgatg ggacttatat taaaattcta taagtcagca caagatgcta
    15601 tcaagcaaaa tgttactata aacgatatta tcaagctttc agttcttgaa aagattggtc
    15661 gtgctaagta tgtagaagaa ggcgatatag atgcagctta cgattcaatt gaagatgaaa
    15721 tagacaacga gcttgcagac cttattaaaa agaggggggc tgaagaacta tgataaagga
    15781 atataagtca atacaagagg tagccggtcc tcttatgtta ataaacggcg tagaaggtgt
    15841 aaaatttgat gaacttggag aaattgaact ttcaaatgga gaaattcgtc gttgcaaagt
    15901 tctagaggta aatggagata ctgcacttgt tcagcttttc gaaagctcaa caggtataaa
    15961 ccttgctgaa agtaaagttc gttttttagg aaagagtctt gactttggag tttcaccaga
    16021 tatattaggt cgtgttttta gtggaatggg tagacctatt gatggaggtc cagaaattat
    16081 accagataaa agacttgata taaatggagc tccaataaat ccagcagcac gtgattatcc
    16141 ggccgagttt atacaaactg gagtctctgc tatagatgga cttaatacat tggttagagg
    16201 gcaaaaactt cctatattct cagggtctgg tttgccacat gccaatcttg cagttcagat
    16261 tgcaagacag gcaaaagtac gtggaactga ttctaagttc gccgttgtat ttgctgcagt
    16321 tggtataact tttgaagatg ctgaattttt tataagcgat tttaaagcaa ctggtgcaat
    16381 cgaccgttct gtagtgtttg taaaccttgc aaatgaccca gctgtagagc gtgtatctac
    16441 acctagaatg gcacttacag cagcagaata ccttgctttc gagcaagata tgcatgtact
    16501 tgttataata acggatatta caaactatgc agaggcattg cgtgaagttt cagcagctaa
    16561 aaaagaagtt ccaggacgtc gtggatatcc aggatatctt tatactgacc ttgctactat
    16621 gtatgaaaga gcaggaaaaa tgaaagggca tgaagggtct ataactatga ttccaatact
    16681 tacaatgcct gaggatgata agacacatcc aattcctgac cttacaggat acattacaga
    16741 agggcaaatt atacttagcc gtgaacttta taaaaaagat atacagcctc ctatagatgt
    16801 tttaccatca ctttctcgtc taaaagataa aggtattggt aaaggtaaga ctcgtgaaga
    16861 ccatgcagat acaatgaacc agctttttgc agcttattca agaggtaagg atgcaaaaga
    16921 acttatggct atacttggtg aatctgcact ttctgagatt gatttaatgt atgctaagtt
    16981 tgcagatgaa tttgagaaag aatatgtttc acaaggattt agaactgata gaactattga
    17041 gcagacatta gaaatcggat ggaagcttct tagtatgtta ccaaaaagtg aacttaaacg
    17101 tatccgtgat gaatattttg aaaaatatat gcctaaggag tgatatctgt ggctagatta
    17161 aatataaatc caacacgtat ggaaatgact agacttaaga aactccttaa aactgctaca
    17221 agaggtcata agcttcttaa agataaactt gatgaactta tgaaacaatt tcttgagatt
    17281 gtaagagaaa ataagcgatt gcgtgaagaa gcagaaaatg ctcttgatac tgcttataaa
    17341 aactttataa tcgcaagagc agttatgagt caggagtatc tgggttcagc acttatgatg
    17401 ccaaagcaat cagtttcagt agatgtttct acgagaaata taatgagtgt tgatgttcct
    17461 gtgtttgact ttaaaactga aaacaatcaa agtgatattt atccttatgg tcttgctttt
    17521 acatcaggtg aattagattc tgctatggaa gctttctcag atgctatgca accactttta
    17581 agacttgcag aaagtgaaaa aagtgcacag cttcttgcac aagagataga aaagacacgt
    17641 agacgtgtta atgctcttga aaatgtaatg ataccaaatt acattgagac tattaagtat
    17701 atagctatga agcttgaaga aaatgagaga gcaagtacta ctagacttat gaaggttaag
    17761 gatatggtgc ttaaaaaagc acttgaagaa aagaaaaaaa atgatttggt tgtataatta
    17821 aatacttata aaaataagtc taacattcta ctttatagag gttagactta ttttttatgt
    17881 ttaaattaaa atgtagatac tataaaaatt aatatattgt ttaaaaacag actgtattta
    17941 actaaaaata atatatttaa caaaaatttc taaaaattaa cgaactgtta atttacactt
    18001 aactttatat taacaaatta ggtgtattat ataaacataa gcaaaaacaa taaaaaacaa
    18061 ataaaaaaag gaaaaaacac aaagggggaa gatatgaaag aatttatatt tacttgtctg
    18121 tgcattgtag tgattacatt tatgacaagt tgcatatcaa caacaccaca aactactcaa
    18181 ggaaaaggaa ataataaact tacaatatat gcagcactag aggaagagca gatagcttat
    18241 tatatggaag gttttagaga agagtatccc aatatagatg tagagatagt tatggattct
    18301 catggtgtaa tagcttcaaa actattagca gaaaaagata atccacaagc agatattata
    18361 tggggattat cagcaatgaa catgatagag ctaaaaaaag atggtgcact tgaagcatat
    18421 aagcctaaaa attatgataa agttgataaa aaatttaaag atactgaaga tgatgtatct
    18481 tgggtaggaa tgagtgtccc agagacagca atagtagtta attataagga acttaagaaa
    18541 ctaggtatag atattccaaa aagttatgaa gatttaataa aaccagaata taaaggactt
    18601 ataacaatgc caaatccagc atcatctgga acggggtatc ttacagtatc agggcttatt
    18661 caaatgatgg gtgaaaaaga tgcttacaat tacatggata aattacataa aaatatagcc
    18721 acatatacac actcgggttc aaaacctgcg aagttagctg gctctggaga gtatccaata
    18781 ggtattacac ttggatatag agctacacaa caattatcta gtggagaacc tatagaggtt
    18841 gtatttccaa aggaaggttc aggttgggac ttagaagcaa atgccttagt taaaaaagat
    18901 aatataaaaa aagaagcaaa gctattttta gattgggcaa taagtgattc agctatggat
    18961 agatatgcac atgaagtagc agttacttcc ataaagacag ataatccagt tccaaaaggt
    19021 tattctaaac aaccatttga acagcttata aaagaagttg atttaaactc tgctgccaaa
    19081 aacagaagta cagtacttaa aacttgggaa tcaaaatacg gttcaaaatc tgaaagtaag
    19141 taaagtttaa attaataatt tagatgaaag aattttagaa gaaaaagtag gatataaaat
    19201 ttaaaataaa aaaataacta tataaggaga gataagatat gagttactta aaaataaata
    19261 atgtgtttaa aagttatgac caaaaaaagg ttttaaataa tattagtttg gatattgaag
    19321 aaggggaatt tctatgttta ctcggaccta gtggatgtgg taaaactaca ttacttagaa
    19381 taatagcagg tcttgaagat gtaaatagtg gaactataat tctccaggat aaagatatta
    19441 caaatttaga gccatcaaag agaggttttg gtatagtatt tcaatcatat gcattatttc
    19501 cgaatatgac tgcttataac aatatagctt ttccactaaa agagagaaaa gtttcaaaag
    19561 aaaaaataga taataaagtt aaggaagtac ttgaaacagt tggattgact aatgaagctc
    19621 ataaataccc taaagcatta tcaggtggac aacaacagag gattgcaata gcaagagcat
    19681 tagctcttga accaaagttt ttacttttag atgaaccaat gtctgcatta gatgcaaagg
    19741 ttagacacaa gcttagaatg gacattaaaa gattacaaaa agaacttaat ataacgacaa
    19801 taatggtaac acatgaccaa gaagaagcta taactatggc agataaaata gctattctaa
    19861 atggaggaga tataatgcaa atagggactc cagaagaaat ataccaaaat cctcaaaacc
    19921 tatttacagc tcaatttata ggagatacaa actgttttga taatggagat tctatattaa
    19981 ctgtaagacc agaatatgtt caaatagaga aatcgacaaa agaaaactac caagggataa
    20041 tatcaaatat agagtttaga gggaatttat tgagagttga aataaaagat aaattaaatg
    20101 aaaatttcat aataagtgat gtttctataa aagagtgggt taatttaaat ttagtagaag
    20161 gtgaccttgt aaaaataagt atagacgaaa aatactattt aaagtatcca aaagttaaag
    20221 gagcataagt gtatgaataa taatgctata agttctaaaa gtataagtct taaatcaaaa
    20281 atgaatttag atataaaaaa atgtatgctt acagtattag ttatgtcttt agtaatattt
    20341 ttattagcac ctataatata tctatttgta caggcttttt tagataaaga tggagtcttt
    20401 gtaggattac aaaactttca agaatacttg gcttctgaat ctctattaaa ttctctaaaa
    20461 aatacagtat ttgtatcagt tatatcaact gtaatatcac taacacttgc attagtatat
    20521 gcatatgcat taactagaac aaagattaaa tttaaaggtt tttttaaatc attagctatg
    20581 tttcctatgt ttgctccaac aatgatgcat gcaataggtc tagtatattt atttggaaat
    20641 caaggtttgg taactaattt acttggagtg catgttccaa tatatggagc agtgggaata
    20701 atattggcac aaattatata tgtatttcct caatcatttc aaatacttta tatatctcta
    20761 agttcaaccg attatagact atatgaagct agtaatacta tggggataag caagataaga
    20821 gaatttataa gcataacaat cccaagtata aaatactctt taataagttc tgcattcgta
    20881 tcgtttacat tatgttttac agattttgga gctccaaagg tgataggagg agagtttaat
    20941 gtattatcaa tagatgtata taagcaaatt atagggcaac agaatatgcc aatgggagca
    21001 actgtatcaa tattactttt aatacctgca ataatatcgt ttatagtaac taaaaaaatt
    21061 gaaaaaaatc aaaattcata tataacttca aagtcaattc catataaggt taaaactaat
    21121 aagttaagag atagtatttt aggaggggtt gtaggaatta ttgcatttgg tataatagct
    21181 gtaatgttaa ctgtaatctt atcttcatta atatcagttt ggccatatga tttaagactg
    21241 acactggaac atttttctat gaaaaacagt attgatggga ttaaaccatt tataaattca
    21301 ataaaaatgt cactatttac agctattcta gggacgagct ttgttttctt atttgcatat
    21361 ttgaatgaaa aaacagaaaa agctccatat ttaaaatcat ttgggtattt tttaagtaca
    21421 ttgcctatgg cattgccagg attagtttta ggtatagctt atgtattatt ttttaataaa
    21481 ttagagttta attttatgga tacaatatac attaagaatt tgtttaatgg attatatgga
    21541 acattactta taatggttat atgtaatata gttcatttct tttcagtagc atttataaca
    21601 gctactactg ctattaagaa agtagataaa gaattagata tggtagctaa atcaatggga
    21661 ataaacagcg catcaattct taagaacgta actataccac tttgtttgcc agcagtttta
    21721 gagaacttta tgtacttttt cctaaactcc atggtgacaa tatcagcatt agtttttata
    21781 tatactgcaa gttcaaaagt agctgcaata tcaattgtac aactagatga taaggggagt
    21841 actgcacagg cagcagcact agctgtgtta atattagtca caaatatatt agtgaaagta
    21901 atatatgaat ttataaagaa tcgtttagaa aaaagaacaa actttaatta aataatttat
    21961 cttataaaaa cacaaattta aaaaagtcta ttcttgtgat ttattattta aagaatagac
    22021 tttttaaatt tattattgat attgtttatt aattttcctt ttgttatcat acatttgaga
    22081 atctgcacga cgatatgtat caaataaaga aacatctact ttttcatcaa aaatagcata
    22141 tccaatagac attgataatt ttaattcatt agttgagttt tcttcaacta tttttaaatt
    22201 a
//
LOCUS       DBGJFU010000062        22104 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.062, whole genome shotgun sequence.
ACCESSION   DBGJFU010000062 DBGJFU010000000
VERSION     DBGJFU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.062"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            444..1514
                     /locus_tag="ACOIAS_002171"
     CDS             444..1514
                     /locus_tag="ACOIAS_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701879.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="ACOIAS_002172"
     CDS             1498..2367
                     /locus_tag="ACOIAS_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGZ2701880.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="ACOIAS_002173"
     CDS             2411..3271
                     /locus_tag="ACOIAS_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGZ2701881.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="ACOIAS_002174"
     CDS             3302..4606
                     /locus_tag="ACOIAS_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2701882.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="ACOIAS_002175"
     CDS             4669..5931
                     /locus_tag="ACOIAS_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HGZ2701883.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="ACOIAS_002176"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="ACOIAS_002176"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGZ2701884.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="ACOIAS_002177"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="ACOIAS_002177"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGZ2701885.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="ACOIAS_002178"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="ACOIAS_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGZ2701886.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /gene="hprK"
                     /locus_tag="ACOIAS_002179"
     CDS             13008..13937
                     /gene="hprK"
                     /locus_tag="ACOIAS_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HGZ2701887.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14151..15947
                     /locus_tag="ACOIAS_002180"
     CDS             14151..15947
                     /locus_tag="ACOIAS_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HGZ2701888.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16087..17868)
                     /locus_tag="ACOIAS_002181"
     CDS             complement(16087..17868)
                     /locus_tag="ACOIAS_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HGZ2701889.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17891..19777)
                     /locus_tag="ACOIAS_002182"
     CDS             complement(17891..19777)
                     /locus_tag="ACOIAS_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HGZ2701890.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19794..20291)
                     /locus_tag="ACOIAS_002183"
     CDS             complement(19794..20291)
                     /locus_tag="ACOIAS_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HGZ2701891.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20609..22090)
                     /gene="cls"
                     /locus_tag="ACOIAS_002184"
     CDS             complement(20609..22090)
                     /gene="cls"
                     /locus_tag="ACOIAS_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGZ2701892.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8499 a         2962 c         3697 g         6946 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaattttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tactttcaaa tcaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa attacattgt gatagcaaac aatgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacctc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt taccatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tggaaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacagaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacaaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttaggctc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaactgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggttag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag ggccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcggg aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga attgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactggg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    14101 ttaattttat atttgagttt cattatataa caagaaagtt ggaatttttt atgggaatag
    14161 aatattttag taaactttta gaaagtagta ctgaaaaaga attgctctta agtaaaaagt
    14221 caaataacat aagtacagca agattaatat cttttttgat agtgatagca gggtttgcta
    14281 ttggatttta caataaaaat atagtaggta tttttgttgg agttttatca ataataatat
    14341 ttatagcact cttagttatt catagtaaag taaaagaaga agaaacatat tttaaaagta
    14401 agagcgaagt attaaataag tatattaaga gatttggaga tggttggaag gaatttaaaa
    14461 tagatggtaa agaatactta aaagatgaaa attcgcaagc aaaagattta gacttatttg
    14521 gaagagcatc attgtatcaa tatatctgtg tagctaatac atcttatggg aaaaattttt
    14581 tagccaaata tttgtggaat gaaaatccag atgagaatgt aatcttagaa agacagagag
    14641 ctataaaaga acttttatca aaagatgatt tttctattca tattcaaaca ctcagtaata
    14701 taattggtaa agagcaaaaa aacaattcag atagtagtat agaatcgttt atagaatatg
    14761 gagaaaataa gaatgtgtat ataccaaaat ggatgcatat ttttacttgg ggtttaccaa
    14821 ctgcaacaat cttatctttt atattttgta tgttaggatt tttacctgtt ttacctgttt
    14881 ttttactttt tataatccag ttagggttta gtggttttgg atatccaaga cttatgcaaa
    14941 ctcttgctcc attattttca tttagtagaa gtatacaagt atatgaaaaa atgtttgagg
    15001 ttttagaaaa agagacgttt gagagtgcat atttgaaaga attacaagat aaactttcta
    15061 agggaagtgg agtttcaaga ggtgttaaac aattaaattc aataggaaat gctgtaaacc
    15121 taaggtataa tcagatacta tatataattg cctgtggtgt tttgatgtgg aactatcatt
    15181 gtgcagaagc tttggagcga tggaaaggta tatatggaaa tcaaattaga gattggtttg
    15241 aatctattgg agaatttgaa gctctaataa gtttaacagt tgtttctcat gtaaaggaaa
    15301 atacatgttt tccagtaata aaatatgaag atactccaag attgaaagtt gaagaggtat
    15361 accacccatt aatagcagaa aaaagtgtca tagcaaattc gatacaatta aattctcaga
    15421 cttgtattat cacaggttca aatatgtctg gaaagacaac atttttaaga agtatcggtg
    15481 taaacttagt gctggcatat gcaggtgctc cagtatgcgc taagagtttt gatgcgacct
    15541 gtatggctat atttacttct atgagaatac aagatgatgt aagtcaagga atatcaactt
    15601 tctatgctga gattcttaga ataaaatcaa tgattcaata tagtttaaaa gaactgccaa
    15661 tgttggttct agtagatgaa atttttaagg gaactaactc tgctgataga attattggag
    15721 caagtgaggc agtaaagaaa ttatcaaaac cctgggttat aagtatggtg acaacacatg
    15781 actttgagct atgtgacctt tcaggtagtg gagatgttga agttgtaaac tatcatttct
    15841 cagagtacta tgtagacgat aaaattcatt ttgactataa gataaaagat ggaagatgta
    15901 aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat tctttaatta atagataggt
    15961 taattaaaga ataagtctat ttagaaatat aatttaattt gtgaaattaa taaggattgt
    16021 cttaaattac gtaaaagtat tttaagacaa tcctttttta agaataaatt ttatattttg
    16081 ttatgactac ttagcttctt cacaagcaca ttcttgtgga cactctccat agacacaact
    16141 cttatcaaag taatgagtat gaaggtattt atgggctaca tcactatttg gttctccaaa
    16201 gaagtcttta taaatagctt gtaaatcagg attctcatga gatttacgaa tagccttatt
    16261 tctatctatt tcatataacg catttgcacg tctatcaact atttcagtat taccatggtg
    16321 ataaggttga ccagcacctc caacacatcc accaggacat gccataactt ctatgatatg
    16381 gtatttacat tctccacttc tgaccttatc cataatcttt ctagcgtttt taagtgtatt
    16441 tacaatacac acattaactg tagttccatc aacatctata cttgcttccc taattccttt
    16501 aagacctcta acattagtga agtttacatt atctaaagtc ttattagtta tcttctcata
    16561 tgaagtacgt agggcagctt ctaaaactcc accacttgct ccaaatatag atgcagcacc
    16621 tgtagatttt cctagaggat tatcaaaatc ttggtcttga agattaggta aatcgattgc
    16681 agcttctttt atcatcttag ctaactctct agtagttata gaaaggtcaa catctagtat
    16741 tccagattca cttaattctt ctcttgaagc ttcatatttt ttagctacac aaggcattac
    16801 agatacaaca taaagttctt caggattgat tcctaatatt ttaggtgcat agtagttttt
    16861 agctatagaa ccaaacatac cttgaggaga cttacagcta gatgctaagt ttaacttgtc
    16921 tggataattg tgctctaaga agtttaccca tgcaggacaa caacttgtta atataggaag
    16981 attttcacct ttttggattc tttctataaa ttcagtagct tcttccatta tagtaaaatc
    17041 tgcaccaaag tttgtatcaa atacatgttc aaatccaagt gcttttaaag cagctaccat
    17101 tttccctgta gagattgacc ctggttctaa tccaaactct tcaccaatag caactcttac
    17161 agcaggagca acttgcacta ctatagtctt ttctttttta tttaaaacat cccaaagttt
    17221 aggtacatta tctacttctg ttaatgctcc agttggacag acagatatac attgaccaca
    17281 gaatgtacac tctgtttcaa ccatatcaga gttaaagaat gtactaacta aagtattaaa
    17341 tccacgatta actccagata atgctcctac tgtttgtatg tcattacata cagtctcaca
    17401 tcttctacat aatatacact tagaatggtc tttaactaaa gatttagtag aagtatcttt
    17461 acctgcaaaa gatttagaac cttgatatct tatttttctt actcctaaat cagcagctat
    17521 agtctgtaat tcacaatctc catttttttc acagacaaaa caatcttgtg gatggtctga
    17581 taacaataat tcaacaatag ttctacgagc atttaatgct ttagcactat tagtctgaac
    17641 tttcatacct tcctttataa ctgttccaca agctggaact aatcctctct ctgtctcaac
    17701 catacaaaca cgacaagaag cacaagtgtc aagcttatct atttcattca tatgtaaatg
    17761 acaaaggttg tgtattttta tatttattaa tttcgcagca tctaagatag aagttccact
    17821 aggtgctgaa acgtgtttgc cattaatagt taaattaact aaactcattg ttattcctcc
    17881 tccaaataag ttatttcttg attatagcgt taaatgtaca attatctata caagcaccac
    17941 attttagaca cttagaagta tctattgtat gttgttcctt aacagaacct gttatacaac
    18001 cagcaggaca cactctagaa cattttgtac aacccttaca gtcatctgtt ataaagtaag
    18061 aaagtaagtt ctgacattta ccagcaggac atttcttatc tacaacatga gctatatact
    18121 catctttgaa atattgaaga gtacttaaaa cagggtttgg agcagcttta ccaagaccac
    18181 ataatgaagc agtttgtata gtttcagcaa ggttttctag gtcatctaaa tcttgtagag
    18241 taccattacc ttctgtaatt ttagttaata tttctaataa tcttgtagta cctatacgac
    18301 aaggtgtaca tttaccacaa gattcttcaa cagtaaactc aaggaagaac ttggctatat
    18361 ctaccataca gtcagattca tcaagaacaa gcataccacc agagcccatc attgaaccga
    18421 tagaacttaa tgaaccaaag tctataggag tgtctaaatg ttcagttggt atacatccgc
    18481 cagatggtcc acctgtttga acagccttaa atttcttacc ttcagggatt cccccaccaa
    18541 tttcaaatac aatatctctt aaagtagtac ccattggaac ttcaacaagt cctacatttt
    18601 ctacttttcc accaagagca aagactttag taccactaga atcttcagta cctaagttgg
    18661 caaaccaatc tccaccctta agtataattg caggtatgtt agcaaaagtc tcaacgttat
    18721 ttaagcaagt aggagatttc catagaccac tcttagaaga actataagtt ttcattctag
    18781 gttcaccacg tctaccttca attgaatgca ttagcgctgt accttcacca caaacgaaag
    18841 cacctgcacc atattttaat tctaatttaa agttaaatcc agtgcctaag atattttcgc
    18901 caagtaaacc gtatttttca gcttgagcta tggctacttt taatctttct atagattttg
    18961 gatattctgc tcttatgtat atgtatcctg tatcagaacc tatagcgtat ccacatattg
    19021 ccatagcttc tagtacacta tgagggtcac cttcaagtac agatctatcc ataaatgcac
    19081 ctgggtcacc ttcatcagca ttacatacaa caaacttttt atctgttgga ccagctggat
    19141 tttttgaagc agcttcccat ttagagccag tagggaaacc tgcaccacct ctaccacgaa
    19201 ggccagaagt ttttacctct gcaataactt cttgaggagt aagagaagtt aaacatttgc
    19261 ctagagctaa ataaccatca ttggcaatat attcttctat actatctggg tctataagac
    19321 cacaattttt taaagctata cgtaactgtt ttttgtagaa agacatttct tcttgttctt
    19381 caactttttt acttaaagat tcttcctcat ataataattc ctcgacaact gtatttctaa
    19441 ttaagtggct ttgaactatc ttttcagcat cagatggctc aacttttacg tagaaaacgt
    19501 tatcaggata aacttttaca ataggacctt gagcacagaa accaaaacaa cctgtcaaac
    19561 gaacactaac tttatcttgg attcctgatt tctttatttc attttctaat tcactaacta
    19621 tttctagact gtttgaagat gtacatccag tatcacaaca aacaagtaat tctcttctta
    19681 atacattttc cttagaataa ttttttctta agctaagatt aggtttcagc tcatcagcta
    19741 aaacttttaa ttcatcaaat gaatttactt tacgcattat ttattcaccc ctcttaacaa
    19801 ttcaattcta attctctata agtatctaat atttcgctta cttggtcagg tttcacttta
    19861 ccataaactt taccatttac agtaacaact ggagctaaac cacatgcccc caaacatctt
    19921 aaaccttcta aagaaaattt aaaatcacta gtagtttctc caacttttat gccaagctgt
    19981 ttttcaaaag cacttaagat tttgtcagca ccttttacaa aacatacagt tcctagacag
    20041 acactgattt tatacttacc tacaggttct gtagtaaaat aagaatagaa tgtaacaacc
    20101 ccatatacct ttgcaggagc tactccaagt tttctagcaa catgaagttg aacttctttt
    20161 ggaagatatc caaatatacc ttgggcttca tgtagaactt gtattaacgc accttccttt
    20221 gttgcaagag agtcaataaa tatatctagt tcgtcaaata attgcttgtt gtgtgaaata
    20281 aaatcgcaca taacaaatcc cccttttctt tttaaagtta ttatttttca taaaataatt
    20341 aaccatgagt tgaccccaac attatgtggg tgaacaaaac caaaacaata aagaaagaat
    20401 aatatacaac aatttcttta tttataaaaa caatgcaaaa aagttaccta ttaaaaataa
    20461 aaactatact agtctattat aatataaaat aattcagaat agttaaaaaa aataaaaaaa
    20521 atgagctaaa ttcaataaaa aaaaaataat acatataaaa aactttaaaa aataaaccaa
    20581 aatgtaaaat ttttataaaa taaaagattt ataaaactgc tgataatagt cttgaaattg
    20641 attccttgat ttttatataa gtagacctag aattatatat atctaatgtt atttccttag
    20701 aatttaaaat atcattttca aatataattc tttgttccaa tgcttttttt gaagagtaca
    20761 taaatgcatt aacttcgaaa ttaagctcaa aacttctgat atccatatta gctgttccta
    20821 tagagcatat agagtcatct ataactattg tcttagcatg taaaaatgca ttttgaccat
    20881 aagtatagat tttagctcca aactttaaaa gttctccagc atatgaatat gatgcccagt
    20941 atacaaaggg atggtcaggt ttagaaggaa tcataactct cacatcaaca ccagataaac
    21001 aagctatttt taaagtatct ataaaagttg agtcaagtat taaataagga ctctgtatgt
    21061 atatatactt tctagctttc tgaatcattt taaggtaacc atatttaatc tcatcaagtt
    21121 ctgatatatc tggaccactt gatactatct gaatcccaat attttcagaa ggatgattag
    21181 aatttgtatc ttcttctata aagtactttt ctaaatctaa gtcttcttta gttgtatatc
    21241 tccaatcaag gatgaatctc atatttaggt caattacgca atcaccagta agcttagtat
    21301 gtgtatctct ccatagacca aattttggat ttctacctaa atattcatca ccaacattat
    21361 atccacctac aaatcctacc ttaccatcaa tgacaacaat ttttcggtga tttctatagt
    21421 ttaagttaaa gtttacaatt ttcaagaatg atgggaagaa gcttccaact ttaacaccac
    21481 tttctttaag tctggaaagt gtactatttt ttagtgttct accaccaact gcatcaaata
    21541 aaagtcttac ttcaacacct tctttagctt tgtctataag aatgtctatt atttcagttc
    21601 cgattttgtc atctttgaaa atgtaaaatt gtatgtttat atatttttta gcttttttaa
    21661 gttcttcaag aagactgtta aaaaattgac tagattcagc ataaatccac acatcgttat
    21721 tattggtata atgagcatta tttgaattag ccaatgcata aatcatatcc ttatgttgat
    21781 gtatatctga atcaatttca gatgtagatt ggagtttaac ctgagtatct agtatattac
    21841 ttttgattat cttatcatcc ttttctttca atctaaacat attgtttttt gaaatatttc
    21901 tgccaaaagc tatataaagt atgaatccaa gagcaggaat caatactaat ataagaagcc
    21961 atgttaaagt tgtatttatg tttctttttt ctctaaagat aaggtttagt ataaccatga
    22021 aattaattat atatattgta gtaactgcta tttcatatat tgacatttcg aaaaatgata
    22081 tatcaaacat aaaattccct cctt
//
LOCUS       DBGJFU010000063        22013 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.063, whole genome shotgun sequence.
ACCESSION   DBGJFU010000063 DBGJFU010000000
VERSION     DBGJFU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.063"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(266..457)
                     /locus_tag="ACOIAS_002185"
     CDS             complement(266..457)
                     /locus_tag="ACOIAS_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701893.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            677..1723
                     /gene="cobT"
                     /locus_tag="ACOIAS_002186"
     CDS             677..1723
                     /gene="cobT"
                     /locus_tag="ACOIAS_002186"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGZ2701894.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            1755..2315
                     /gene="cobU"
                     /locus_tag="ACOIAS_002187"
     CDS             1755..2315
                     /gene="cobU"
                     /locus_tag="ACOIAS_002187"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGZ2701895.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            2325..3098
                     /gene="cobS"
                     /locus_tag="ACOIAS_002188"
     CDS             2325..3098
                     /gene="cobS"
                     /locus_tag="ACOIAS_002188"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGZ2701896.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            3118..3732
                     /locus_tag="ACOIAS_002189"
     CDS             3118..3732
                     /locus_tag="ACOIAS_002189"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGZ2701897.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      3799..3978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4093..5607
                     /locus_tag="ACOIAS_002190"
     CDS             4093..5607
                     /locus_tag="ACOIAS_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HGZ2701898.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            5646..7022
                     /locus_tag="ACOIAS_002191"
     CDS             5646..7022
                     /locus_tag="ACOIAS_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HGZ2701899.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            7023..7994
                     /gene="cbiB"
                     /locus_tag="ACOIAS_002192"
     CDS             7023..7994
                     /gene="cbiB"
                     /locus_tag="ACOIAS_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HGZ2701900.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            8026..9096
                     /locus_tag="ACOIAS_002193"
     CDS             8026..9096
                     /locus_tag="ACOIAS_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2701901.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEEREYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            9098..9985
                     /locus_tag="ACOIAS_002194"
     CDS             9098..9985
                     /locus_tag="ACOIAS_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HGZ2701902.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            9973..10605
                     /locus_tag="ACOIAS_002195"
     CDS             9973..10605
                     /locus_tag="ACOIAS_002195"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HGZ2701903.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            10614..11840
                     /gene="cbiD"
                     /locus_tag="ACOIAS_002196"
     CDS             10614..11840
                     /gene="cbiD"
                     /locus_tag="ACOIAS_002196"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HGZ2701904.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            11837..12445
                     /locus_tag="ACOIAS_002197"
     CDS             11837..12445
                     /locus_tag="ACOIAS_002197"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HGZ2701905.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            12435..13010
                     /locus_tag="ACOIAS_002198"
     CDS             12435..13010
                     /locus_tag="ACOIAS_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HGZ2701906.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            13003..13755
                     /locus_tag="ACOIAS_002199"
     CDS             13003..13755
                     /locus_tag="ACOIAS_002199"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HGZ2701907.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            13739..14860
                     /gene="cbiG"
                     /locus_tag="ACOIAS_002200"
     CDS             13739..14860
                     /gene="cbiG"
                     /locus_tag="ACOIAS_002200"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HGZ2701908.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYHVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            14857..15582
                     /gene="cobJ"
                     /locus_tag="ACOIAS_002201"
     CDS             14857..15582
                     /gene="cobJ"
                     /locus_tag="ACOIAS_002201"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HGZ2701909.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            15579..16331
                     /gene="cobK"
                     /locus_tag="ACOIAS_002202"
     CDS             15579..16331
                     /gene="cobK"
                     /locus_tag="ACOIAS_002202"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HGZ2701910.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            16350..17828
                     /locus_tag="ACOIAS_002203"
     CDS             16350..17828
                     /locus_tag="ACOIAS_002203"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HGZ2701911.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            17858..18763
                     /gene="hemC"
                     /locus_tag="ACOIAS_002204"
     CDS             17858..18763
                     /gene="hemC"
                     /locus_tag="ACOIAS_002204"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGZ2701912.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSAIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            18753..20252
                     /gene="cobA"
                     /locus_tag="ACOIAS_002205"
     CDS             18753..20252
                     /gene="cobA"
                     /locus_tag="ACOIAS_002205"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGZ2701913.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            20269..21234
                     /gene="hemB"
                     /locus_tag="ACOIAS_002206"
     CDS             20269..21234
                     /gene="hemB"
                     /locus_tag="ACOIAS_002206"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGZ2701914.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            21387..21965
                     /locus_tag="ACOIAS_002207"
     CDS             21387..21965
                     /locus_tag="ACOIAS_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HGZ2701915.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT         9056 a         2112 c         4154 g         6691 t
ORIGIN      
        1 gggattattt aagaaatttt gtattataaa tagaaggaaa tagataaatt gtatagaata
       61 aaatatatta agataagtta ataatgttaa atgggaagtg agtttatgga aaaaccaaat
      121 acaaaagttg ttaactttca agagtataaa gacagaaaaa accaaagaga agtaaataga
      181 gacgaacttt taaagcttat aaaaaaaatt gtgacttcaa aatagggagt tgttaaactc
      241 cctattttaa tgggtttcat aataattaag acattctgag tccatctata taatctttga
      301 attgtgagaa atctgattca ctacttaatt ctatggtttc tgaaccttcc gtataaaaat
      361 tcttaagata gatagtataa ttattatcaa cacaatcatg ataaatacat ctatatccat
      421 cttcatacaa actttttaac ttagaaaaat catccacaat atcatctcct taaagttctt
      481 ttaaatatat ttttacaaaa agcatctaat tgtatgcaaa ttgacaatgt aaaataaagg
      541 aaatgaacat aaaatgtaga ataattttta tatgtatgta aaaataaaaa gaaaaagttg
      601 aaatttttag aaaataagag tattttttat ctaaaaagag agtttaaaaa ataaaaaaat
      661 gttgggagga taaaaaatga gtttattaga aagtatatca aaaaacatat attccctaga
      721 taactcttct attgaaaaaa caaagcagag attggatagg cttatacatc caactggaag
      781 cttaggaaag atagaagata tttgcatgca attagcagga atatttggca atgaaaattt
      841 tgacacaagt aaaaaggtta taattgcttt tgcgggagac catggtgtat atgaagaagg
      901 cgtagcacct gaccctcaaa atataacaaa attacagttt ccaaattttt cgaaaggtct
      961 atgtggagtt ggagtaataa gtaaatttgt aggggcagat gttgtagctg tagatgtagg
     1021 aataaactgt gatgaaaaac tggatggggt attagactac aagataagaa aagggacatc
     1081 aaatatggca aaaggtccag ctatgagtaa acaagaagca attagatgtt tagagatagg
     1141 tattgaaata gcagagcagt gtatagaaaa agattataag gttattggta ttggggagat
     1201 gggtatagca aatactactc caagtacagc aataatatct gtaatttcag ggtgtgaccc
     1261 gttagaagtt actggtatag gtgcaggtct taagaaagaa agactaaagc ataaggctga
     1321 agtaattaga aaggctattg aaattaataa tccaaatcca actgatggag tagatatttt
     1381 atctaaagta ggagggtttg aaataggctc aatggcaggt gttatattag gttgtagtgc
     1441 taataggatt ccagtagtaa ttgatgggtt tatttcttat gcagcagcat taattgcata
     1501 taagataaat ccaaaaacta gagaatatat gatagcatct catttatcag cagaatcagg
     1561 aactaaaaga gcattagaca tattaaaatt agaccctcta ttaaatatgg atatgagatt
     1621 aggtgaggga agtggagctg ctttagcatt tgatataata gaagcttcaa actatactta
     1681 taaaaatatg gcaacttttg atgaaattga catgggaaga taaatataaa aatttaatat
     1741 gtaggagtgt tttgatgagt aaaattattc tagtaacagg aggagctagg tcagggaaga
     1801 gtaactttgc agaaaaacta tgttttgaca gaaataataa tacagcttat atagcaacct
     1861 ctattccatt tgatgatgaa atgaaagata gagtaaaaaa acatcaagag aatagaccta
     1921 gaaactggaa tacttatgaa atatatgaag atatatattc cataattaag gagatatata
     1981 aagaacatga aactgttata ctagattgtg taacattatt agtaaataat ctaatgtttg
     2041 aacacaatct aaatatagaa gaatctagtc aagaagaaat aaacaaatta gaacaataca
     2101 taaaagaaca agttgtcaaa cttattgaag aaatagaaaa aacaaatcta tactttgtat
     2161 ttgtaacgaa tgagttaggt atgggaattg ttcctgggaa taaacttagt agaatttatg
     2221 ctgatattgt aggtcgtata aatcaatata tagcttcgaa aagtaatgaa gtttattttg
     2281 tagttagtgg catagctatg aagataaagg agtagtatgc aattatgaaa agatttattt
     2341 taatactaca gtttttgaca aggataccta taaaattaaa tgtaggattt gatgatgaat
     2401 tttataagtc aatagtatat tttccacttg taggttttgt cataggtata ttatcctatt
     2461 taataggatg gatttctatg cttttatttg aaccatttat agcttctata ataataactc
     2521 tagctggtgt cttaattact ggaggacttc atatagatgg attaggagat acatttgatg
     2581 caatatatag ttatagggat aaagaaaaga tgcttgaaat aatgaaagat tcaagattag
     2641 gtacaaattc acttctagca attatgtttg tacttttatt aaaggttgga ttcgtttacg
     2701 atataataag taataattca ttgtgggtta taatttttat gccaatgatt gcaaggttag
     2761 gagtaatgtt attgacatat aaaactgtga caccaagaga aaaaggaatg ggaaatttat
     2821 ttataggaaa attaactaca agtatgttaa taactgctat aatatacaca ttattgatag
     2881 ttgctcttat tactaaattt atatttttat tacctaatat agtgttgatt aaagtattag
     2941 gttcgattat agtggttttt gtatttataa tattattcaa aaaacatatt tataaaaaaa
     3001 ttgacggagt aacaggagat atattaggct gtggaataga actttcagag cttgtatatt
     3061 taatttacat atatttgtta atttttatgt ttttttaaag taaaaagtgg tgataatatg
     3121 ataagattaa tattaataag acatgcactg acaaatgata ataaaaaagg aagattatca
     3181 ggccatataa atagttgtat tagtgaagag ggcaaactac aaataaataa aattaccaga
     3241 tatctgagta atgaaaacat agataaggtt tatacaacac catctacacg tactaaagat
     3301 acagtagaaa aaatatcaaa attgaaatta ttagaaatag aagaaaaaga agctttaaga
     3361 gaaattagtt ttggagactt tgaaggaaga acttttgaag aaattaaaat caaatatcca
     3421 aatgagtttg aaaaaatgat aaaagaaggt aacaactata gatatccaaa tggagaaagt
     3481 ttgatagact cttataaaag agtggcaaaa gaaattgata atataatttt agaaaatgat
     3541 agtaatttag atacaaagac aatacttata tgttctcatg caggaacaat aagaaatata
     3601 ataacacatt tgatttctgg tagttataag tatcactgga actttaaaat tgataatgca
     3661 tctattacag ttttagaaat tgatggtgga tttgctgtaa tagataaaat gaattttaca
     3721 gacttcatat aatttaatca aaaccaaaca ttacaatctg tgtttgtaat atatctgtaa
     3781 aattagcaat gaaaatagaa tattagatta aaattaggtt cttaattaga tgttaaaagg
     3841 gaaaaaggtt agatgccttt gcagcccccg ctactgtgaa accaacgaaa ctataaatac
     3901 cactgtcagt ttgatgggaa ggttatagaa gtaggatgaa gttaagccag gatacctgcc
     3961 taatttaatt taaaacaaga ggtcttcggg gtgaaagatt tttaattaat atatataaaa
     4021 gtcctatctt tagaagtatt aggacttttt ttaatttgtt ttttagtaaa ttttaacaag
     4081 gagggatgga aagtggggaa aaaaataatg ctacaaggaa cagcttcaaa tgtaggtaaa
     4141 agtgtcttag tagcaggttt gtgtagaata tttaagcaag atggttatac tgttgctcct
     4201 tttaagtcac aaaatatggc tttaaattct tttataacta aagaaggatt agaaatggga
     4261 agagcacaag tatttcaagc agaggctgcg aaaatagagc caatagctga tatgaatcca
     4321 gttttattaa aaccttctgg aaataataaa agtcaagtta ttgtaagagg taaagttgta
     4381 ggagaaatgc cttcttctaa gtatcatgat tataaattgg agttagtaga tgtgttgaag
     4441 gaaacattta atagcttaaa ttcaaggtat gatattgtag ttatggaagg ggcaggaagt
     4501 aatgcagaga taaacttaaa agatagagat atctctaata tgggtatggc agaaatagct
     4561 gatgcacctg taataatagt cggagatatt gatagaggag gcgtatttgc ctcattagca
     4621 ggaacaatgc ttttattaaa tgaagatgag aaaagaagag taaaaggtgt aataatcaat
     4681 aaattcagag gaaaaaaaga gcttttacaa gatggtgtaa agatgctaga agacatcata
     4741 aaagtacctg ttataggagt agtaccttat acagatataa agatagaaga tgaagacagt
     4801 gtaactacga gatttaagaa aaaaatgaat aaaagtgata tacacataga aataattaga
     4861 actccacata tgtcaaattt tacagatttt aatatatttg agacacaaga agatgtaagt
     4921 attagatatg tagactatgg agaatcttta ggaaatccag atatattaat aatacctgga
     4981 actaaaagta ctatagacga tttaacatat ataagaaaaa gtggtcttga atcgcaaatc
     5041 aaaaatttac atagtcaagg taaattgata tttgggatat gtggtggata ccaaatgtta
     5101 ggtaaaaaat taaaagaccc ttatcatgtt gaaagtgaaa ttgaagaagt tgatggaata
     5161 ggtcttttag atacagaaac aacgtttgag aaagaaaaga ctacaacaca agtagaagct
     5221 actattgttg aatgtattag tgaatacatg aataacctta agggaaaaaa agtaaaaggt
     5281 tatgaaattc atatgggaat tacaaagcga ggtaatgatg taagaagttt agatttaata
     5341 actaaaaaac ttgataaaga agtgaattat actgagggaa gtgtaaataa agaggggaat
     5401 gtaattggga cttatcttca tggaatattt gatgaaataa gctttacaag agcaatttta
     5461 aacaatataa gaaagaaaaa aggtttagga gaattagaaa gcaaagtaaa ctcttttaat
     5521 gaatttaagg ataaagagta tgataagttg gctgattttt taagagaaca tttagatata
     5581 gagaaaatat atgagattat gaaataatga tgacctttat ttataattta ggtaggaggt
     5641 gtattatgaa aaaaatttta attgcgggta ctaatagtgg agtaggtaaa acaacaatat
     5701 cattaggaat aatgcaagct ttggttaaga gaaatatgaa agtacagcca tataaagtag
     5761 ggccagacta tatagaccct tcatatcaca cttttataac tggaagacat tctagaaatt
     5821 tagattctta tatgttagat gatgaaaaaa taaagtatat agtaaataag tcctcaaaag
     5881 atgcagatat ttcagtaata gaaggtgtta tgggcttata tgatggcttt ggaatagatt
     5941 tagataattg tacaagttca catacttcaa aggtattaaa gtcacctgtt atattagtta
     6001 taaatggtaa atctatggca gcatctagtg ctgctatggt acttggatat aaggaactag
     6061 ataaagatgt aaatataaag ggtgttatag taaataatgt aaagactaat agccattatc
     6121 aaataattaa gagttctata gaaaaatatt gtaatgttga agtattgggg tattttcctc
     6181 caaataacaa attctctttg gaatctcgac atctaggatt agttcctagt gtagaaatga
     6241 aatcactaag ggaaaaattt tataccttag cagatgaaat tgaacactat atagatattg
     6301 acagaataat tgaaatatca gaaagcgatg aatttgagag tgattttgat tttcaaactt
     6361 taatagaaaa taataaaata aaagaacatg ttaataataa atcaatagct atagcatatg
     6421 ataaagcatt caatttttat tatagagaaa atatagagct tttagaggaa cttggtttag
     6481 aaataaacta ttttagccct cttgaagatg tatcagtgcc aaatgcagat tatatttata
     6541 ttggaggagg ttttccagaa atatttggaa aagaattaga agcaaatgag tcaatgagaa
     6601 cttccatatt agaagcacat aacaataata tacctattta tgcagaatgt ggaggactga
     6661 tgtacctagg tgagaaacta ttgaatcaag aagaacaaga atttaatatg gttggaatat
     6721 ttagtggaat tagtaagatg acatcttctt taaaaagatt tgggtactgt attggtgtag
     6781 ccaaggaaaa tactctttta tctaaaaaag gggaatctat taaagggcat gagtttcatc
     6841 attcaatatt tgaaagtgat gaaaaatgtg tatattcaat gagaaaagaa agagatggaa
     6901 atatagtaga agagtgggat ggtggttata gtaaaggaaa cactttggct acatatcttc
     6961 atactcattt ttataataac ttaaattgta tagaaaattt tttgaaaaaa ggaaatgaat
     7021 aaatgagtat tttatctatt tatataggat atgtgacaga cttaattgta ggagacccat
     7081 attcttttcc tcatccagtg agatttattg gtaaacttat aaattttact caaggtataa
     7141 taagaaaggt ctctaaaaat gataggcagt taaaattggg gggatttata ttatggttta
     7201 taaccgttgg aataacatat ttaacaactt atgctatagt gaagttattt agttttaata
     7261 tattattgag tattgtagtg aatagtttta taatttatac aacactagcc acaaaatgtt
     7321 taaaagacga agcattaaaa atttataatg tattaaaaac tgaagatata gaaaaatcta
     7381 ggattcagct atcttatata gtaggtaggg atactactaa tttaagtgaa cctgaaataa
     7441 taagagcaac tgtagaaact gttgcagaaa atactgtaga tggtataatt gcgcctatgt
     7501 tttatgcatt tattggagga gcaccacttg ctatggccta taaagctatc aatacattgg
     7561 actcaacagt tggatataaa aataaaaagt ataaagatat tggttttgca tctgctaaga
     7621 ttgatgatat agccaattat ataccagcta gaatatctgt cattcttatg acaattggaa
     7681 gtttcttttt aaattataac tataggaact gttttaaaat ctctattaga gatagaaaga
     7741 atcataaaag tccaaattgt gctttttcag aaggtgcagt atctggtgct ctaggaatac
     7801 aactaggagg taccaacgtt tattttgggg aaaaagtata taagccaaca ataggggata
     7861 agcttaggga gatagataaa gaagatataa ttaaaactaa taaaattatg tatgcatctt
     7921 catttgtatc tatgttggta tttacactaa tatatgtgtt atgtaggatg attttaatta
     7981 acttaattat ataaattatt gtttgttaac ttaaggggga catagatgaa ggatttaggg
     8041 catggtgcca atgtagatga aatggcaaga ttatatggga aaaacccaaa agaaataatt
     8101 gattttagtt caaacataaa tccaaatgta ttaccaaatt tagagagata cattttaaaa
     8161 ggattagaag agtgtaggaa ttatccagat ataaattata caaacttgag agaaaatata
     8221 tcaaaatata tagatataaa tccagacttt ataatacctg gcaatggtgc aacagaagtg
     8281 atttatttgc ttatgaagag tattaaaaaa aaattggcta taataaatcc gactttttca
     8341 gagtatagaa gaagtgctga gttaaataat ttagatatta tagatttaga gttagattta
     8401 gaaaataact ttaaacttaa tatagatata ataaaagaaa atataaaaag atttgatagt
     8461 ttatttatat gtaatcctaa taatccgagt ggtaatgtcc aagatttaaa ggaacttgtt
     8521 catttactag ataaacataa taaggtatta ataattgatg aaactttcat ggaatttgtt
     8581 gaagatgaaa gtaaatatag tttggttaaa tatatagaat caaataaaaa tatatttata
     8641 attaaagctg tgactaagtt ttttggtatg cctggtttaa ggctaggata tggtcttaca
     8701 agcaacactg aaattatgaa caaaatttat gaacacaaag agccatggac tatcaattct
     8761 tttgcagata tactatccaa ttttattttt gaagataaag agtatattaa aaatagtaaa
     8821 gaatattaca tagaagaaag agaatatatg ttacaagagt tgagaaacat aagaaatata
     8881 aaggtctatg atacagatgc taattttatc ttgataaaaa tatataaaaa aacaactaaa
     8941 gaattgaaaa aagatttgtt taagcaagga aacatattag ttagagatgc gtctaatttt
     9001 ataggattag atgatagttt tataagagtg gctataaaat ctcatgaaga taacaaaatt
     9061 cttatagaaa atatgaaaaa tttattgggt gattaaaatg aaatcttatg gaatatgccc
     9121 agcatcttgt ggagaatttg tacaaggtat aattgatgat gaagaatatt tatgttcata
     9181 tgcaatagat atgtattcta aagtgtatat tgaagaaaaa ttagtagata taaatttagg
     9241 gagatataag tcaaggttag caattgaaaa ggtatttgaa aaatttaatt tacctaaaaa
     9301 atatactaaa aatatttctt taaatataaa tagtaaaata cctgttggaa aaggaatggc
     9361 aagttcaact gctgatatag gagctactat aaaggcaaca ttatcattga tagataaaga
     9421 tttaagtagc gaggagattt ctaaattagc agcagaaata gaaccaacag attctatatt
     9481 tatagataaa aatagtatat ttaatcctct aaatggaact gttataaaat acctaggaaa
     9541 tctgaccaat gcgaaagttg taatacttga accaaacaaa gtactagata ctatgaaaat
     9601 tagattgagg caagattata ataaattaaa agtagagaat aaagaagtaa taaaaaaatc
     9661 atttgctctt ttagaagaag gattgaaaaa aaataattta tctctagttg gagaggcttg
     9721 tactttgagt agcttagcaa atgagaatat agaaaaaaaa gagtatttaa atgaaataat
     9781 aaaaatatca aaaaaatatg gagcatatgg agtaaatatt gctcacagtg gaactgtagt
     9841 aggtatttta attgataagt ttatgaatga taaaaagatg atagatgcct tatgtgaatc
     9901 taacataaat tctgtctata ataaaattta tacacaaaac ataattaatg gaggaattaa
     9961 gggggaaata gaatggaata cataaagaat cctatgaaaa tagaagaaaa aagttttgaa
    10021 ttgatacaag aaataataga cgaaattaga ccagattata aatttaagaa taatatcgaa
    10081 gaaaaaataa taaaaagagc tatacatact actgctgatt ttgattactt agatatatta
    10141 aaaatatcag aagaagcagt tgatagtata atagatgcat taaaaaataa tgcaagtatt
    10201 tatactgata caaatatggc tcttagtggt ataaataaaa gaaagttaga atcattagga
    10261 tgtaagtata aatgtttagt ggcagatgat gaaactgtaa aattagctaa agaaaaagga
    10321 attactcgtt ctatggcagc agtagaaatt gcagctaaag aagaaggaag aaaaatattc
    10381 gttttaggta atgctccaac agctttatat aaagttatgg agatgaaatc agaaggtaga
    10441 ttagatttag atgcagttat aggagttcct gtaggttttg taggagcaca agaatcaaaa
    10501 gatgaagttg aaaaaacaga tataccatat ataatttcta aaggtagaaa aggtggcagt
    10561 aatttagcag cagcaatagt aaatgctata ttatatacta tgtaggtacc tatatggaag
    10621 aatatgtata tatagatgga aaaaaatata gaagaggata tactacaggt tcatgtgcaa
    10681 caggtgcatc taaggctgct gtatatatgt tgattacaaa aaatagaatt aatactataa
    10741 atatagatac tcctaagggg ataccattac tactaaaagt tgataatata aatatttctg
    10801 atacatttgt ggaatgttcc ataaaaaaag atggtggaga tgatatagat gcaactcata
    10861 ccatggatat ttatgcaaga gctgaaattg tagctaaaaa tgataagaac aaagggtatc
    10921 ttactctaaa ggatatagat agtttaagca ccaatagtga gtgtaaaagt gagctctata
    10981 agtttattag ggtttatggc ggaactggta ttggtgtggt tacgaaaaaa gggcttagtg
    11041 tggatgttgg taagcctgct ataaatccga ctccacttaa aatgataaat catgaaataa
    11101 gaaagcttat aggagataat tttgaatcta tacttggaaa tgataaagtt ttaaaaataa
    11161 caatctttgc tccacaagga gaaactgtag caaaaaaaac atttaatcca agattaggta
    11221 tagttggagg aatatctata ataggtacaa ctgggattgt tgaacccatg agtgatgatg
    11281 gttggaagaa gtctctatct atagaacttc agatgaaaaa agaacaaggc ttagataaaa
    11341 taatattggt tccaggaaat catggagaac agtttataag agaaaaatta aacttagata
    11401 ttaagtatgt agttagggta agtaatttta taggctatat gattaaagaa gctcaaagaa
    11461 taggatataa aaaaatctta atggcaggcc atataggtaa atttataaaa gtatctgctg
    11521 gtatttttaa tactcatagt aaggtagcag atgctagaag tgagatatta gtggcaaact
    11581 tagctttaat gggagctaga tacgaatttt taaataaaat aaatcaatgc gtaactactg
    11641 aagaagctgt agagctaata aataatagtg aatatagaga agtatataat atattaagta
    11701 ataaatgtag ggagagagta aagcaatact tgaatgaaga ttcagatgat attgatgtgg
    11761 aagtaatcat attttcaatg gataagtctt tattaggtaa atcggataat acagatgatt
    11821 tagtggaggt ctttatatga taaacataat aggtctagga cctggtaact tagactatat
    11881 aacaaaaaaa ggtgaaaatt taatttccac ttctgatgta ttaattggtg gtaaaagaaa
    11941 tttagagtct ataaaaaatt ttgaaggaga aaaaatagtt ctggattcta atttaagaga
    12001 aattatagag tacataaata acaataaaga aaagcaaata tcaataatag catctgggga
    12061 cccattaata tatgggatag gtagatatct atctaaaaat attgataata agatgttaaa
    12121 catggtttct gggattagtt cattacagta catattttca aaaatatatg tagatatgaa
    12181 tgatgtatat attacaagta gtcatggcaa agtaccagat tttgattaca tattatcaca
    12241 taaaaaagta tgtatggtta cagattcaaa gataggacca aaacaaataa gtagagaaat
    12301 aatagataga aatctaaata aaattattgt tgtaggagaa aatctgtcat atgacaatga
    12361 aaaaataaca atagcaaaac cagaagagat aataagaatg gacaattttg atatgaatgt
    12421 tgtggtgatt ttagatgagg aatagtgaat ttataacagg taaagttcca attactaagg
    12481 aagaggttag agcaatatca ataagtaagt taaatttagt aaatgctaaa aacctcatag
    12541 acataggggc tggtacagga agtgtaagtg ttgaagctgc atataattat cctaatttaa
    12601 aggttatatc tatagagaaa aatgatgatg ctatagagct tatagagaaa aataaaaaaa
    12661 aattcaactt aaaaaatata gaagtgataa aaggatatgc tccaattaat ctagggttaa
    12721 atgttaaagt tgagtctatt tttcttgggg gaacaggaaa taatctggaa gaaataatag
    12781 aatggtctaa aaaaaatctc gttacaggag gaagacttgt agctaatttt ataataatag
    12841 atacatttaa tcaaacttta aaactgctta aaaagcatgg atttaaagaa atagatgtat
    12901 gtgttttgaa tgtttctaaa ctagagaaac taggaaaagg tgaatatttt aaaccactaa
    12961 atcctatata tataatatct tgtgaaaaag gggaaaatga tgatgaataa agtacatttt
    13021 gtaggagcag gacctggaga taaagagctg ataacattaa aaggatacaa attattaagt
    13081 aatgcagatg tagtgatata tgcaggttca cttgttaatc ctgaactttt ggaatactgt
    13141 aaagaagatt gccaaataca taatagtgca catatggatt tacaagagat aatagatgtt
    13201 atgagagaag gtatagaaaa taataaatct gttgtaagac tgcaaacagg tgatttttct
    13261 atatatggtt caattagaga acaagtggaa gatttaaata aattgaatat agattatgac
    13321 tgtactccag gagtaagttc ttttttaggg gcagcatcat ctttaggggt tgaatataca
    13381 gttccagaaa tttctcaaag tgtaattata acaagaatgg aaggcagaac tccggttcca
    13441 gagaaagaat caatacaatc atatgctaag catcagacat ctatggtaat atttttatct
    13501 gttcaagaaa ttgagaaagt tgtgtctaaa ttactggaag gtggatatcc aaaagatact
    13561 cctatagcag ttatatacaa agcaacttgg gcagatgaaa aaatagttaa aggaacttta
    13621 agtgatatag ctgtaaaagt taaagaaaac aacataaata agactgcttt aataatggtt
    13681 gggagatttt taggagaaga gtataataat tcaaagttat atgataagga tttcaaacat
    13741 gaatatagag ggtaatattg ctataatctg tattacagaa aatggaaaaa aattagctct
    13801 aaagataaat tctctagtaa ataactgtgt tatatatcat gtaaaaaata aaaaaagtaa
    13861 tttagttgtt gaagaaacat gtgtgcatac agttcaaaag aagttaagtg attttgtagg
    13921 agaaatattt agcaaatttg attacatagt atttataatg gctacaggta tagttgtacg
    13981 aagtatagca ccatacataa taagtaagtt tagtgaccct gcaattttgg taatggatga
    14041 aaaaggcaaa aacgtaataa gtttattgag tggtcatatt ggaggggcaa acagcttgac
    14101 acagtacata agtagtctta ttgatgctaa tccagtaata acaacagcta ctgatataaa
    14161 tcaaaaagct tccttagata tgatagcaaa aaaactaaat gcacatattg ataattttag
    14221 agaaagtgta aaagaagtaa attctatgct agtgaataat aaatgtgttg gattatatat
    14281 tgatgaagga tatcaagttg atactagggg atttaaactt atagaaagta aggatgagtt
    14341 aagagatagt ttttcaaaca catatataga aaaattagtt gtagtaagca ataaaataaa
    14401 ttgtattgaa gatattgaga atttaaacct agataataaa tttgaacatt gctatagtac
    14461 caaaaaaata ataaaggtaa ttccaagaga tatagtgata ggaattggtt gtagaagaaa
    14521 cacagatagt gaactgatgt ataattcact tgaagagttt cttaataaac aaaatataga
    14581 tattagagca ataaacacag ttggaagtat agaactgaaa aaagacgaaa aagcaataat
    14641 tgaattatca aaaatattga aggtgccatt taaaacattt tcagtagatg aaatatctaa
    14701 ggttgataat ctttttgata aatctgattt tgttaaaaaa aatgtgggcg tgtattcagt
    14761 agcagaacct gtagcatatc ttttgagcaa aggtaatttg ttagttgaaa aacataaata
    14821 taaaggaata actttttcag tggggagaat aaaaatatga tatacgttgt aggaattgga
    14881 ccaggaagta aagatacaat gactttagaa gcaataaaag caatagaaga ttctgaggct
    14941 attgttggat ataagactta tataaaacta atagaagaat ttatacaaga taaagaaata
    15001 atccaaaatg gtatgaggca agaagttgat agatgtaaac aagcagtaga ggaagcaaaa
    15061 aaaggaaaaa aagtagcagt tgtaagcagt ggtgatgctg gaatatatgg gatggcaggt
    15121 cttattttag aattaatatc taaagaagat gaagatataa aagtaaaggt aattccaggt
    15181 gttactgcaa gtattggtgc agcagctata cttggagctc ctattatgca tgatttctgt
    15241 catataagtt tgagtgattt aatgactccg tgggaagtaa ttgaaaaaag attgagattg
    15301 gccgctgaag ccgattttgt aatttgtttg tataatccaa gaagtaaggg aagaagtgaa
    15361 catctaagca aagcatttaa aataatgggt gagtttaaag gtggagatac acctgttgga
    15421 gtggtaaaag atgtaggtag agaaaaagaa gaaaagtttg tatgtacttt tgaaaatatg
    15481 gactttgaaa aagtcgatat gactacaatg gttataatag gaaataagtc tacctatatt
    15541 aatggtgaat tgatgataac tccaagaggt tatacagtat gattttaatt ttaggaggaa
    15601 cttcagatag tttatctata tgtgataaga taaatgaact gaaaaatcaa ccatatatcc
    15661 tttcagttac tacagattat ggaagggaac ttgcacaaca acatgcagag aatgtaattt
    15721 taggtaagct tggaaaagaa gatatgctaa aatttataaa agataataat atagtaaaaa
    15781 taatagatgc cactcatcct tatgcagttg aagtatccaa aactgctatt acttgtgcaa
    15841 aactattgaa tatagattat ataaggtttg agagaaaatc tttaattgaa gaaatagatt
    15901 atgataataa atttatagta gatacaattg aagaagcctg tgaaatagca aataaaatag
    15961 ggacaaatat attcatagga acaggaagta aaaatttgaa cagatttata gaggaaataa
    16021 gtgataaaaa tttagttgtt agagttcttc caacgagtga agtcatatta agttgtgaaa
    16081 aattaggttt aagtgcagat aatatagtag ctatgaaagg tccatttagc cagtatataa
    16141 atgaagaaac atataaacat tacaatatag atttagtcat aacaaaagaa agtggaatag
    16201 ctggtgggtt cttagagaag gtaaatgcat gtaaaacttt caatatacca gtaataatta
    16261 taaaaagaga atcgattaac tacgaaaatg tatttaatga cataaataaa attaaagaca
    16321 tacttgtgta atttttagga ggagaatata tgaagaaagc cttattagta gtgagttttg
    16381 gtacaagcta tcatgaaacg agagaaaaga caatagatat atgtgaagat aaaataaaaa
    16441 attcgttaaa aagccatgat ttttttagag cttatacatc aaatatgata ataaataaaa
    16501 taaaaaaaag ggatggaata gaaatagata atccaataca ggctttagat aaaatatatg
    16561 aacaaggata tgatgaagta ataatccaaa cacttcatat tatatgtggc gaagaattta
    16621 acaaattaaa agaacaagta gatggatatt cttctaaatt caaaaagatt gtattaggaa
    16681 gacctttgct tacacatatt gaagattatc aggaagctgt agaggcaatt aagcatcaaa
    16741 ttcctcatat ggaaagtaat gaagcagtag tatttatggg acatggaaca cttcatgaat
    16801 cacattcagc gtatccagca ttggaataca tgcttaggga taatggaata aatgcttatg
    16861 ttggtacagt tgaagggtat ccagaaatag aacatgtaat aagaagatta aaagaaggaa
    16921 atataaaaac tgtaaatttg atgccattta tgttagttgc aggagaccat gctataaatg
    16981 atatggcagg agatgaagaa gattcttgga aaacaatatt agaagaaaat ggatttaacg
    17041 taagagttca tttaaaagga ctaggagaaa attcttatat acaagataaa tttgttcgtc
    17101 atgctgtaaa atgtgcagag aatgcgaagt tctatggtat aggagcaggg ccaggagatg
    17161 gttctttact tacgataaaa gcggttaata ctttaaagaa actggatatt ctatacactc
    17221 cagaatcaaa aaagggtgga gatagtctag ctttatcaat agttagtgaa tatttacctg
    17281 aatcagtgga aataaagtct aggcatttcc caatgagttt tgatgggaat gaaaaaagtt
    17341 tagcttggga caatgtggca caagaaatta tatcagatgt taatgatggt aaaaatgtag
    17401 gatttgttac attaggagac cctatgattt atagtacata tgtatatata atgaaaagaa
    17461 tattaggaga ggtagaagtc gaaactatac caggaatttc atcattttcc aatatagcat
    17521 ctaatcagaa ttttccatta gtcatggata aagaagcttt agtggtaatt ccttgtacta
    17581 tggaagatga taagatagag tatgctttgc aaaattataa ttcaatagta ttgatgaaag
    17641 tatataaaaa ttttaaagaa ataataaaaa aattagaaaa gtatgattta atagaacatg
    17701 ctattttagt aagtaattct tcccaagaaa gcgaaaatgt gtttacagat ttaaaagaag
    17761 cgcaccttga agataagata tcttattttt ctactatact agtaaataaa gataataaaa
    17821 taaaataggt gaaagcaata tttaaaggag acttaatatg aatatagtgg ttggaactag
    17881 gggtagtaat ctagcgctta tacaaacaga atgggtaata aatgaattaa aaaagaagta
    17941 tccagaaatt agttttgaga ttaaaatcat aaagacaaaa ggagatttaa ttcaaaatgt
    18001 ttcactggat aaaataggtg ataaaggatt gtttgtcaaa gaaatagagc aacaattatt
    18061 agatggaaaa atagacatag cagtacatag tatgaaagat atgccttctt atttagcaga
    18121 tggactaaaa tttgctcata cgcctaagag agaagaccca agagatgttc taatacttag
    18181 agaaggatat aaaaatttag atgatttacc tcatggagca gtaataggaa caggaagtaa
    18241 aagaaggaag tttcaattat tgaaacaaag acctgattta aatatagtac aagttagagg
    18301 taatgtggaa actaggatac gaaaaataaa agatgaaaac atgcatggta ttgtgttggc
    18361 agcatcagga ataattagag ctaatttgca agataaaata agttcttatt tacctgttga
    18421 tgttgttatt ccagcacctg cccaaggagc tttagctata gaaattagaa gcaatgatag
    18481 tgcgatagag ggaatagtaa attcactgaa ggatgagaat acagaaatcc aaatattggc
    18541 tgaaagaggt tttttagatg gtgtaaatgg aagttgtcat attccaatgg cagcttattg
    18601 tgaaataatc caagataaaa tacatcttac tggcttatat ggagattcag aaggaaaaaa
    18661 agtagtaatc aagtctatag atggagatat ttcttctcct agagagttag gtttaaagtt
    18721 agctaaactt gtattgaagg agtatgagaa ttatgaaggg taaggtttac ttagtagggg
    18781 caggaccagg tgattataaa ctcatgactt taaaaggttt agagtgtata agaaaatcgg
    18841 atgtaatagt atatgataga ttagcaaata gtagttattt aagagaagct aagcctgatt
    18901 gtgaacttat atatgtaggc aaggcatcaa gtaatcatat tttgccacaa gaagatataa
    18961 atagaattat agcagaaaaa gctaaagaag gaaaaattgt aactcgtctt aaaggaggag
    19021 acccttatgt atttggcaga gggggagaag aagcagagac tttagtagat gaaggtatag
    19081 aatttgaagt agttccaggt ataacttctg ctataggagg tttatgttat gcgggtattc
    19141 ctataacaca tagagaccat gcttcgtcat ttcatgtaat aacaggtcat ttgaagggtg
    19201 atgatagtgg agaactaaac tggaatgctc ttgcaaataa caaaggtact cttgtgtttt
    19261 tgatgggaat ttcaaaccta gagaaaataa gtgaaaactt aatgaaagaa ggaaaagata
    19321 aggatacacc tgttgctctt ataagttggg caactagata taatcaaaga gttgttacat
    19381 ctacacttga aaatgtttat gaaactgcta ttaaagaaga agtaaaaccg ccaactctta
    19441 tagtagttgg gagtgttgta ggacttagag aaaagttgaa cttctttgaa agtaagcctt
    19501 tatttggaaa aagtattgct gtaactaggt ctagaaatca aaatagtgta ttggtagaaa
    19561 aaataatgga tttaggtgga aatcctatag aaatacctac tattaaggta gaaaaaatac
    19621 aaaataatat taacttagaa aatgaaataa aaaatataaa taagtataat tatctaatac
    19681 taactagtaa aaatgccgta gaaatcttct ttgaaaagat atatgaaatg aattttgatt
    19741 taaggatatt atctaattta aaggtatgtg ctataggttc tgctacatca aatgagttga
    19801 aaaaaagagg aattatagcg gatatagtac caaaaaaatt tgtagcagag tcactgtatg
    19861 aagagttagc gcctatttta aatgatgacg ataatatact tatacctaga gcagaaaatg
    19921 ctagagatta tctggttgac aaacttaagg ctatttgtga agtaacagaa gtacatatat
    19981 atagaacagt aatagatgag gagaaaaaag gtgagatatt agatatttta aattctaatg
    20041 acatagatta tataacattt actagttcct caacagtaaa taatcttgtt gaaattatag
    20101 gaaaagaaaa tattaaaata ttagaaaaaa taaaaacact atctataggt cctataacta
    20161 cagagacaat aaatagtttg ggaataaaat tgtacaaaga atctgctaca tctactatag
    20221 attcgttagt agatttaatt ataaaaaatt aattttggag gcttgaatat gttaagaaga
    20281 ccaagaagac taagagcaag taaagcaatc agaaatttgg taagagaaac taaggtgaat
    20341 gtagaagact taatatatcc actgtttgta gtagaggggg aaaatataaa agaagaaatt
    20401 aaatctttac cagatgtcta tcatttatca atagatatgc tagaagacga aataaaagaa
    20461 ataagtgagt tgggaattga acatataatg ttatttggtg ttccagatga aaaagatgag
    20521 tgtgcttcac aagcttttaa tgataatgga atagttcaaa aggcaattag aaaggtaaaa
    20581 gaaataaatt cagatataaa tgtaataaca gatgtctgta tgtgccaata tacaagtcat
    20641 ggacattgtg ggattttaac tgatactggt tatgttgata atgataaatc attggagtat
    20701 ttggctaaaa tatctgtaag ccatgctaaa gcaggagctg atatggttgc tccatctgat
    20761 atgatggatg gaagaataca agcaataaga gaagctttag atgaaaatgg attagaaaat
    20821 gttggaatca tggcttatag tgttaagtat gcttcaaatt attatggacc ttttagagag
    20881 gcagctcatt cagcaccatc ttttggagat agaaaaacat atcaaatgga cccagcaaat
    20941 agcaatgaag ctataataga gactgaactt gatatattgg aaggtgcaga tatacttatg
    21001 gttaaacctg cattatcata tttagatatt ataagaagag ttaaagataa ttttgactta
    21061 cctctagcag cttataatgt aagtggagaa tactctatgc ttaagtttgc tgtgaaggaa
    21121 ggtttattaa atgaagatgt aattcttgaa tcaattacat ctataaaaag agcaggagca
    21181 gatataataa taacttattt tgctaaagac attgctaaac tgttgaaaaa ataaattcta
    21241 gcagtataaa gaatatcaaa aagtatagga attgtaatta actattatac agtagttttg
    21301 tttagacatt tattgaagaa taatagaaac taatttgcaa tttctatact ttatttatgt
    21361 aactaaataa aatttggagg tagaagatgc tatatcctat taatttaaaa ttagatgagt
    21421 tagatgtagt tataattggt ggtggagaag ttgcatatag gaaatgtaaa aattttttag
    21481 agtttaacaa aaatgttact atagtttcta aacaaatttt aaataaattt tatgatttaa
    21541 aaggaaatat aaaaataata aaagatgatt ataaagaaat atatataaaa gatgcttctg
    21601 taataattgc tgccacaaat aatagagagt taaatatgga aataggatta tattgtaaag
    21661 caaaaaataa gctagtgaat gtagttgata atatagaaat atcgaacttt actgttccat
    21721 catatataaa aagaggagat ttacttataa gtgtgtctac tgggggcaag agtccttcat
    21781 tatcatcaaa gataaaaaaa gaaattgaag agagatatac tgaagattat gaggagtatt
    21841 taaatgtatt aggagatata agaaaaaagg ttataaaaaa atacgaagat aaaagtaaaa
    21901 gaaaagatgt gcttaatatg ttggtaacac ttgacctaga agaacttaag aagtttgata
    21961 tataaaaaat aaaataaaaa atgttaaaaa ggtgttgacg gattattttt aag
//
LOCUS       DBGJFU010000064        21695 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.064, whole genome shotgun sequence.
ACCESSION   DBGJFU010000064 DBGJFU010000000
VERSION     DBGJFU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.064"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(134..2791)
                     /locus_tag="ACOIAS_002208"
     CDS             complement(134..2791)
                     /locus_tag="ACOIAS_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2701916.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKHYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(3150..4094)
                     /locus_tag="ACOIAS_002209"
     CDS             complement(3150..4094)
                     /locus_tag="ACOIAS_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701917.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(4087..5112)
                     /locus_tag="ACOIAS_002210"
     CDS             complement(4087..5112)
                     /locus_tag="ACOIAS_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701918.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(5187..6755)
                     /locus_tag="ACOIAS_002211"
     CDS             complement(5187..6755)
                     /locus_tag="ACOIAS_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2701919.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGVFWNIADWTIE"
     gene            complement(6868..7779)
                     /locus_tag="ACOIAS_002212"
     CDS             complement(6868..7779)
                     /locus_tag="ACOIAS_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701920.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(7763..8749)
                     /locus_tag="ACOIAS_002213"
     CDS             complement(7763..8749)
                     /locus_tag="ACOIAS_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701921.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            9067..9510
                     /locus_tag="ACOIAS_002214"
     CDS             9067..9510
                     /locus_tag="ACOIAS_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2701922.1"
                     /translation="MILLKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIV
                     IKLIAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTT
                     GFEFATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            9513..9911
                     /locus_tag="ACOIAS_002215"
     CDS             9513..9911
                     /locus_tag="ACOIAS_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HGZ2701923.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(10361..10585)
                     /locus_tag="ACOIAS_002216"
     CDS             complement(10361..10585)
                     /locus_tag="ACOIAS_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HGZ2701924.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(10620..11987)
                     /locus_tag="ACOIAS_002217"
     CDS             complement(10620..11987)
                     /locus_tag="ACOIAS_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2701925.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTAMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(12071..13606)
                     /locus_tag="ACOIAS_002218"
     CDS             complement(12071..13606)
                     /locus_tag="ACOIAS_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HGZ2701926.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDAILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(13629..14063)
                     /locus_tag="ACOIAS_002219"
     CDS             complement(13629..14063)
                     /locus_tag="ACOIAS_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HGZ2701927.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(14323..15234)
                     /locus_tag="ACOIAS_002220"
     CDS             complement(14323..15234)
                     /locus_tag="ACOIAS_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HGZ2701928.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            15530..15883
                     /locus_tag="ACOIAS_002221"
     CDS             15530..15883
                     /locus_tag="ACOIAS_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGZ2701929.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(16102..17043)
                     /locus_tag="ACOIAS_002222"
     CDS             complement(16102..17043)
                     /locus_tag="ACOIAS_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HGZ2701930.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(17144..18379)
                     /locus_tag="ACOIAS_002223"
     CDS             complement(17144..18379)
                     /locus_tag="ACOIAS_002223"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGZ2701931.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSQSKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLI"
     gene            complement(18423..19277)
                     /locus_tag="ACOIAS_002224"
     CDS             complement(18423..19277)
                     /locus_tag="ACOIAS_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701932.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(19405..20580)
                     /locus_tag="ACOIAS_002225"
     CDS             complement(19405..20580)
                     /locus_tag="ACOIAS_002225"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HGZ2701933.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(20679..21563)
                     /locus_tag="ACOIAS_002226"
     CDS             complement(20679..21563)
                     /locus_tag="ACOIAS_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGZ2701934.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT         6980 a         3838 c         2192 g         8685 t
ORIGIN      
        1 agacacttta aaacactgtt atttcaatca tttttatcat caaaacttag atactttgtc
       61 taagtatatt tatatgtgtc taatattaat attattttag actaaaaaaa aattaccaaa
      121 taagttttta tactcattgg taatcttttt cgattatttc tgcatattct aatattaata
      181 atatttgata gcactctttt tcattaattt ctatattata ttttttatct aattgagata
      241 ctatatcttt tattttgctt agactcttgt gataatcaag tgttttttct ttatcaagct
      301 tttcaatttc tctagtattt ccaatcaacc catctaccat gaaagctaaa tgcatcataa
      361 gtccaattac tttatcgtat ttcttttcta tattaaatac taaaaacaat tcatcagaca
      421 catattttat atctttaaat attctattat aattagagct ttttaaggat gtctttaaac
      481 cttcaactac atcatcataa cctatactta tctttataat attttttaat ttattgatat
      541 tttcatcctt atctatatct tttttgctaa tatagtcaat tcctctaata ttaattcttt
      601 gttcattgat tactgcaaca atattactac tttctttaag tttcgtaata tttaaactta
      661 aatctttttc tgttttatat gggacagata taatatctat tccatctaag tacctactca
      721 tcttttcttc taatattttt cttgttttat tatctacacc ttctgaaaca tgtgatgcta
      781 taataattgt tttagacaga ttttctttat tttgaatttc attagttcca aaatagtgct
      841 ttctcgtgtt agtgatagag ttgtatacgt catttaaagg taatcccata ttggccttct
      901 gaactgcttc taacacagtt aatgtcgtca ccataggaat tactcttact ccacactttt
      961 tttctttctt tataatttca tcaaaggagt ttaatgaacc catatctgta aatagtaata
     1021 catctttatt gtctatatta ttaattaaat ttttaaaatc tacaataact tcatcatagc
     1081 ttttatctaa cttcatatcg aaagaaatag cactgcactt tccaataata tcaccaactg
     1141 cttttaccat actacttgct gtagtttctc catgcatagc aactataata ttaatgttat
     1201 tcactatttt attttgttca tcttgtttag cttgtactat gaataatgta atatatgcta
     1261 cagtttctat agataaaaat aaatcaaatt cattttcaat tattccaact atttttaatg
     1321 ctgttttaaa ttcacttctg ttatttcttc taatttgatt caaatctatg ttgtttgtag
     1381 agctactttt atccaatacg ctttttatat aaattgccat tgacttaaat attgattcac
     1441 ttatgtctat ttcttcatct tctttaataa tttgtcttat tgattcacac atgcttttaa
     1501 acttaacatt attaaataga gaagcatctg ctatattcat ttctacagat ttattaagat
     1561 tagtatttac tgcattaata atatttatat cattgtagtt aataacttct tcttctccta
     1621 atgacgtaaa tacaaaatat tctattccat gtgttataac ttcatctgct ttaatagact
     1681 tctctctata atcatcactc acttcattta tagatttatc aaaatatttt ttctctatat
     1741 aaactggttc tttattatct cttttatatc ttagatatcc atttgcaaca gctaatttta
     1801 tatcattttg aagttgacct acattatttt tacaatcata aagtaatagt gatttaatta
     1861 tttctttatg tacttttatt gaaatagaaa tactttttga ttcttccttg aaaaaatttt
     1921 tgactaattc cattctttca atcattgtcc tatctctcaa tgatggtatt tctattttca
     1981 ttggtattct tctattgaat gtatctaata gtgttgatgt cggattttct gtagtagcac
     2041 atattattaa taccttagac tctctttctt tcgttgcatc tcctagtctt ttaaatgttc
     2101 ccttatctat aaaagtaaac aacatttctt gaccttctgc tggtagtcta tgaatttcat
     2161 ctaaaaatag tatccctcca tttgcaattt ccaaaagacc ttttctggaa gattctgcac
     2221 ctgtataagc tccttgttct accccaaaaa tatgtcccat aagtaattgt gcattattag
     2281 cataatcagc acaattaaat gttacaaaag gagctttatt ttttctaatt ccactatcta
     2341 tagaataatc gtacatatat ctagcaatca tactctttcc tactcctgtt tctccataga
     2401 taatagtatg aagtccattt gtaggataga gcattgcgac cttgcatttt tctattatag
     2461 gtcttaaact atgttcttta cctattagtg tttcaaaact atcttttttt attttgtttt
     2521 ctacttttgt tccttttttt atgccaagca actcactttg tgtatcaagc aattcattct
     2581 tgttaaggtt aagatttgca gggatataat acacaggtct tccttttatc ttcgtaacca
     2641 taccctcatt gaataattgg tttaaatatg tacttatatt gtttcttgca attcctgtct
     2701 tttctgataa ttggttagct gtaatccctt tttcattttc cctatatgat gattctaaaa
     2761 ctttcattac tttatctttt ttatatatca ttacaagctc ccctacatta tagtatttta
     2821 ttaactaaaa cccctataca cactttattt tttaataaac tcttattctt taactaaggt
     2881 tataatttat caaactttcc ttactttaaa ttatagaaaa ttattgtaga aaaaataaga
     2941 ttattgctca ttttttctac attactattt ttaattttat agtactttct taacaacata
     3001 ctttacttgt ttttaatttt attatatttg aatttattat atttgaaaag tagaaataaa
     3061 aaaccctatt ttggagtagt tttaaaattt ctccaaaaat agggttttag tttgtatttc
     3121 aaataaatac ctgttcaagt atttttatac taaacaactt ttaaattttg ttgatattta
     3181 tttatttctg atttagaaga atatacaaaa tgtccatctg aaacctcaat ccaacttaat
     3241 gacttttcat ttgaacaatc atatccatta gaattatact ctattctatt tcttgactta
     3301 gcatatctag ggtcaggaac aggaattgca gaaagtagtg attttgtata tggatgaata
     3361 ggattattat acaattcatt tgaatctgcc aattctacta attttccatt atacataact
     3421 cctatacggt cactaatatg tctcaccata gataaatcat gagctataaa taaacaagtt
     3481 aaattctttt cttgttgcaa ttttttaaat aaatttacta tttgtgcttg tatagacaca
     3541 tccaaagaag ctataggctc atcagctatt ataaattctg ggtctactga aagagctcgt
     3601 gctatgccaa tacgctgtct ttgtccacct gaaaaatcag atggatactt atttgcataa
     3661 cttctatcaa gtcctactag ctttagcatc tcatatactt tattcattct atctgtttta
     3721 tttttacaga taccttgaat ttttagtggc tctgaaatta tatctgcaat tgtcatacgt
     3781 ggatttaatg atgaagtaga atcttgaaaa ataatctgca tacttttgtt tacatctttt
     3841 tttataaatt tgtacgtttt tttatctgat atgcaatttc ctttgtaaat tacttctcca
     3901 ccagtagatt catgtagtct tattatagtt ttaccagttg tagattttcc tgaacccgac
     3961 tctcctacca atccaaatat ctcaccctta tatatatcaa aacttatatc atcaactgct
     4021 tttacagtag tacttttgtc caaatgaaaa tattgcttta agttttttat ttccagtatt
     4081 ttttctctat tcattagaaa tcacccttcc acaatttact ctaactggta catctacttc
     4141 aggtgcatct gggtgcaata accaagttgc tgcactatgt gtatcattaa ttttaaacat
     4201 tggtggctct ttttcataat ctatcttaag tgcattttta ttccttattg caaaagcatc
     4261 tccttttggt gggttcaata aatttggagg cattccaggt atattatata aatagtcatc
     4321 ctgcgaatct aaagtaggta atgaacccaa caacccccaa gtatatggat gtctagggtc
     4381 ataaaatata tcctctacag ttcctatttc tacaatcttt cctgcataca taactgctat
     4441 tctatcagct atagttgcaa ctactcctaa atcatgagtt ataaatatta ttgacaaacc
     4501 tattttatgt tgtaaatcct ttattaaatc aattatttga gcttgtatag ttacatctaa
     4561 agcagttgta ggttcatcag caatcaatac atctggatta caagcaagtg ctatagctat
     4621 tactatacgt tgtctcattc cacctgaaaa atgatgtgga aattgtttaa atcttttctc
     4681 aggattatca attccaacca attcaataag ctctatggct ctcttttttg cctcactttt
     4741 acttatacct tgatgaatga ttacagcttc tgcaatttgc tttcctattg atatagttgg
     4801 attgagagat gtcatagggt cttgaaatat cattgatata ttctttcctc ttatatcttc
     4861 catctctttt tcagacttct taaccaaatc acttgattta agtaaaacct ctccattttt
     4921 aatatatcca ttgtatggga gtatcctcat caaactctta cacagtacag atttcccaca
     4981 accagattct ccaactatag ccaatgtctc accttttttt agattaaaag atacatttct
     5041 tacagcctga acttctcctt cttctacttt aaaacttaca gatagattat taacctccaa
     5101 taaatgttcc aaaaaccatc aacccctatg ctattcaaac cttagggata tatttttatc
     5161 cctaaggttt ctaattaatt tcagtattat tcaattgtcc agtcagctat gttccagaat
     5221 acacctacac catgatgacc taaaactgta tcaggtgtta atccttttat gtttggtttt
     5281 ccaacatata ttgcatctat ataagcaata aatgtataag gcatatcctt agtcatttct
     5341 acttggaatt gtttatataa ctttaatttt tcatctttat cttctgtttc tcttgctttt
     5401 tgaagtattt tatctatagt aggattagag taagcactgt aatttgcacc tttatctgtt
     5461 ccaaatactt tatatgtatg gtcatctggg tcaaatggac ttccccaacc tattaaatga
     5521 gcatcttgat tagcccaatc tgtttcagtt acaacaacag ctttagcatc aacaccaatt
     5581 tcttttaact gttgtgcaca tattttagcc atatcaactc taacttggtc actctcacct
     5641 gctgttatct cgaaagctaa cttagtacct tctttttcat agattccatc agaacctaat
     5701 ttccaaccta gcttttcaat ttcttgttta gctttttctg gattatattc aaacttttct
     5761 atatcaggat tattatatgg tcccatttgt aaaggtgaat atgctggtac accatgtcct
     5821 aataatacac tgtcaactat tgcttttcta tctattgcat agcttaaagc atttggtaat
     5881 cctttagctt ttttatcttt aaagaattta gaattaaaat tgtataatat acctctatag
     5941 tcggctgttt tcattatatt tactttaaaa ttcttttcgt ctttttcaaa gttagacata
     6001 tctttaggag ttatttgagc taggtctaat tctcctgatt ttaattgcat tgccttagcc
     6061 ttatcatctg gaacaatctt aaagactact ttatctaatc ctggttcttt tacaaaataa
     6121 tcactattct taacaagtgt tatactttga cctttgtccc atttttcaag tttaaatggt
     6181 ccagtaccta ttggtttttg attaaattca tcagtagcta tatccttacc ctccaaagca
     6241 tgttttggta atactcctac tgttaaataa tcaagcattg ctgtatttgg agcttttaaa
     6301 gtaattttta tagtattatc attaaccaca tcaatttttg ttatatcttc ataatttgat
     6361 gctatctcag aagcattatc tggattcatt atagtttcta ttgtaaactt aacatcattt
     6421 gctgtaaatt tctctccatc atgccactta acatcatctc ttaaattaaa agtatatgta
     6481 tttgttgcct catcaaattt ccagtccttt gctagacaag gaaccacctt attattttca
     6541 tcatgagctg ttaatccatt aaatattaac gaatttattt ctccatgttc atatagtgca
     6601 ggatttatac ttgtatagtc attacttcca taaacaagca cctttccatc tttaggcgta
     6661 tctgccttct tatctttgtc tcctccacta gaacaacctg ccattagact aagtatcatt
     6721 actaaactta aaacttttaa cttctttaat ttcatgatgc tccccctttt gctttttata
     6781 aaatattttt caaagctttg accccattta tttaaataga tatttcaaat acctcagtat
     6841 ttgcttattt aatactcaaa tgctgattta taaattacta gattttttat tattattctt
     6901 tcttatataa ttacctatat ttgttataca caccaatgtg gtaaccaaaa atatacccgg
     6961 aatcagtata atccaccatc tatttgaaag taatgcttcc tctgacaaag ataacatact
     7021 tccccaagaa acaatttcta ttggtaaacc aattcctaaa aagctaagtg tcgattcagt
     7081 accaatagct gcgcctatgt ttgttacaac catgaacatt attgatgcca caaaatttgg
     7141 aagtaaatgt tgctttaata catagaagaa tcctcctccc atgctcttag ctgctagtat
     7201 gtattcgcta ttttttattt gtctaacttc tgttctcact atcttagata tattcatcca
     7261 acttgttatc cctattacta tggacataga tactggattt gaatttccaa gtatagcttg
     7321 tacaaatatt ataattaata ttgaaggtat actaagtatg atttctgtaa acctcatcat
     7381 tgcatcatct acgagttcag atgccgaacc acttatacta ccataaacaa ttcctataac
     7441 agttgatatt acagtggaaa atagtcctat aaataaagat atttttcctc cataccatat
     7501 catagaatat atatctctac ccattgagtc tgttccaaat aaaaaagttt tatttggagc
     7561 taaattagaa cttgctaaat ccatgtatgt tggttcatgt gtcattataa aagatgagaa
     7621 tacacttcca atgactatga ttgataaaat aataattgat atatatggta attctttaat
     7681 ttttttagat aaggttttct ttgtttctat atgttcatct tctgctacta aaatataatt
     7741 ttctcctaca atttcaaaat cactatattg tggcttcatt agtatcacct ctatcatatt
     7801 tcattctagg gtctatttta ttgtttataa tttgagctaa catattccca aatactacta
     7861 aagcccctgt tattaagcat aaaactaata acatattata atcatgatac tttgcacttt
     7921 caaaacaaag tgttcctaac ccaggataag aaaaaacttt ttcaactaca taagttcctc
     7981 ctagtatatg aggtatcgaa atggccatta tactaatata tgatggcatt atatttctca
     8041 ggcaatgctt aaaaacaata gttctattat ttaaaccttt tgctttacat aaaagcacat
     8101 aatcttctct tatttcctct agtaatttat ttcttatcat atatgtgtaa taccatagat
     8161 ggcttaatat aagtactgta agaggtaaga ttagatgtga tatcctactt aatatactgg
     8221 actcttctcc cattgaataa gcaccactac ttggaagtat acttaagttt atactaaata
     8281 taagtatgag tactaaggca acccaaaatg atggaataca gttggttatt gttcctaatt
     8341 tgcatatgat tctatcaatg agcttatctt catgtaatgt acaaaatatt cctaacaata
     8401 aggctagcac aaaggtaagt atatatcctg aaccacctaa tattatagtg tttatataga
     8461 catcatttat aacagaagta acattttgtt tatatttaaa tgaaattcca aattcccctt
     8521 ttgatgcatt tgaaatccat tttatatatt gactgtatat tggttcatcc aaaccaagtt
     8581 ttttcatagc attttctttt tcttgtgtac tcattctttc tactccatcc ccatagtaag
     8641 agattaatgg gtctcctgga gcaagtcttg ccatgtaaaa aacaataaaa gataataaaa
     8701 acattactaa aataaactgc aacaactttt tcagtatatc ctttaacaaa ccccaacccc
     8761 cctttgttgc aataaaaaaa accacataaa agcaaacgta aagtaacgtt aaaaccttcg
     8821 tggttccatt atcattaaac aaaattaaat tttctatatt tatctatgag ttaattttag
     8881 catcatttat taactgtgta aacaaacttt ttaaaaattg ttaaaatttc atcaaattat
     8941 tttatttata cctaaaataa aatttttata ttctattctt cattattttc tataaaatta
     9001 taaaatttta ggttgactat ataagaaaag tatattatat tagtttgtat gcaaaccatt
     9061 taagaggtga ttttattgaa aagtaattat gactgtttta gaatagccat gctcttaaaa
     9121 gaactttatt ctaaaactat gtacacagta gaagaaaatt ttaaagaaaa tggattaaca
     9181 caccaacaga ttatagttat aaaactgata gctcataatc aagaacttac aatatcacaa
     9241 ctatgtgatg aaatgtcttt agctaaaggt acggtttcag gcataatcag cagattagaa
     9301 cagattggat atatagaaaa attcaagaaa tctaatgata aaagaaatac atacgttaag
     9361 tttacaacaa caggatttga atttgccaca aattttaaaa ttaaaatgca agaaagtttt
     9421 gatgatattt ttaaaaattg tgatgaaaac gaattgagtg acttagtaaa aaatcttaga
     9481 aatatattgg caaaagtaaa ggagagataa ttatgaatta taaattaata cttgcaatag
     9541 tatttataac atcagcttta attttctaca ctataggtgt atttggagaa agaaaggcaa
     9601 aaattttaaa aaagaaacat gtaattatat tttggcttgg ttttatattt gatactttag
     9661 gaacatttac aatgagtaac atagccaaca gccatacttt tgaagtaaaa tctgctttat
     9721 cacaaaacct tcatagtatt acaggtcttc ttgcaattgt gcttatgctt tttcatgcaa
     9781 gttgggctac ttttgtccta tacaaagatg atgaggaaaa gaaaaaattc tttcataagt
     9841 ttagtatagt tgtatggact atatggttag ttccatattt tataggtatg tttattggta
     9901 tggctggata gtaaaataaa aagtgtgtaa gaaatactgt aggtaaattt gtataagaca
     9961 aatttatcta catccttctt tcacactttt acatatttat ttataatact ccattatttc
    10021 tttcatgttt cttaatacct atatagaata tttaattatt tctatagtat tttattattt
    10081 tctcacactt tttcatatat atattgtttt actatttctt catatgtata tatgaaatat
    10141 tccattattt ctataaatgt tttattatct ttctcatact ttttcatata tatattgctt
    10201 cactattttt tcttatttct tcatatacat atgaaatatt tcattatttc tataatgttt
    10261 tattatcttt ctcatacttt ttcatatata ttgcttcact atttttttta cttctttatt
    10321 aaatacttat gtataacata catttaataa acgctaacta ttaaacacct aatacttgag
    10381 ttactcctgg aacttcactc accaaaacat tttcaataat agctttgatt gtcatagtcg
    10441 ctcctggaca cccactacat gccccttgta gtctaactaa aaccacacca ttttcattta
    10501 catctataag ctctacatct ccaccatctc tttgtaatac tggttttatc ttttcctcaa
    10561 gcactttttc aactttttct ctcatagcta ttccccccag aaatatttta ttagcaaaat
    10621 tataccaatg gtttaaattt ttgtccatct aatgatttat caaattcaat ttttgcttca
    10681 tcaataattt tagtatccat aaatacatca taagcactac aagccagtac tttagctgaa
    10741 tttatcatag cagaaaatcc tatatttgaa cctgctgatg aacatgactg ccatgtatga
    10801 cttgcaattc caacaggcca agctgccatt gcaaattgag ccactggtat tacataactt
    10861 acatctccaa catcagtaga accagctagt cctttatctt tgtcaccgtc aactatttcc
    10921 ttgtgtatat agctttctac aatagattta tcattttgta atgatgttgc tacacctaat
    10981 ctttcttctc tagtaagagt atcacacaac ttttttgcaa attcttcttc ttcaggagta
    11041 tttttagggc aaccaacaaa aaccatattg tcataaacta aatcagtcaa acattggttt
    11101 ggtatatagt catatattcc atctgtaacc ttatactcca tttcagtttc tgtcatcatt
    11161 gctgctcctt gagctacttt tattaatcta tcaagaacgt gttctgcatc ctttgctttt
    11221 ttacctctaa taaagtacca actttcagca aatccaggaa ctacattagg tcttccacca
    11281 ccattagtta taacatagtg cattcttata gagtcaataa catgttctct caaataatta
    11341 gctccaacat tcataatttc aacagcatca agtgcgcttc taccattatg aggagcttga
    11401 gctgcatggg ctgttatccc tttaaattta aattttacag ataagtttgc cagacttcca
    11461 cctctccaag gtgcatttat atcaaaagga tgccatgtaa atgcacaatc tacatcatca
    11521 aaacagccat ttatacacat agctgtcttt cctccacctt cttcttcagc aggacaacca
    11581 aaatatttta ttgttccatt aaatttattt tcttctatta atttttttat agaaacaact
    11641 gctcctacac ctgctacacc caacaggttg tgcccacatc catgtccagc ttttccttct
    11701 accaatggct ttctttctgc acttacacat tgtgatagct ctggtaaagc atcatattct
    11761 ccaagtatgc caatcacagg tttaccctct ccaaaacttg ctataaatcc agtaggtaaa
    11821 ttttctactt cttcaatttt aaatcctttt gactttaggt attctttttg aatgctactt
    11881 gcatactttt cttcaaaggc taattctgga ttttcccata ttttcttaga taaatcaatt
    11941 aattcttctc ttatttcttc aacattattt atgatatact ctttcataat tttcctccaa
    12001 atatatgtat ttttgatatg ctttacagat tatattatat actaacattc tatttattca
    12061 caattttttg ttaattaatt ttattcatat gctcttctat ggagtatatc ttatcttcac
    12121 tttgcctctt cataaattta atgtactcta tcttccataa agtagtttca gttttctcat
    12181 ttaacataaa ttctaatata ttaatttctc tcattctaat atcttcataa aatactacac
    12241 tacccaatat tgccaattct aattcactta atgatgcaaa caacattaaa tactcttctc
    12301 caaataaaca ttcagattca agaatatcga taaagtcttt tttagcattg atattttcct
    12361 ttattattct tattttttca attatagatg tactatctaa tatctcattg aatatctctc
    12421 taaacgattc atcatccaat ttactttcat catcaacaat ataatcttct aagtgttcct
    12481 ttttattttt actcacagtt accatacttt taaaagtgtt actttttatt gagcttagca
    12541 cagattcaat aaatatcttt tcatattttt taatatatct tattacatct tcatcttcta
    12601 tgcttaatct ttttattaat acttcaattg cttccataag tagtaaagtt atttttttat
    12661 ttattctaat ttctctgtta atttcttcaa aatcattata atccaaatta ctatctactt
    12721 cagtgttaaa actatcatct acttcaatca attcaattat attactttct tcagagtcta
    12781 taactttatc ttcagtaata ttattgtaca tatttttaaa ttgtttttct aaaaattcat
    12841 actcatattt tgaaatttct aaattagtca cttcatttcc taaaatagta gaaaaaatac
    12901 aattatttat aataagttca aataaattta caagtatagc atcacaatct attttataag
    12961 tcaccccata gtcataaaat aatttttctc tatctttttt cttaaatagg ttacaaaatc
    13021 tattttctat atctatactc tcaatataat tcttcacata ataaataccc ttaacatcca
    13081 tatcatctag tgccaatgga taatctatac ttactggaac gtcctgagct tcaaatttta
    13141 caatataact tttaaagaaa cttgataact ccatcaaggt atcattatat gctattgaat
    13201 tagtatccaa ttttctactc atcattagtt tataaaaatc ttttagccta actaactctt
    13261 cctctaatat ttcaatgcct ccaaggtaca cttttttaac attcttagca gtcaactcca
    13321 caactctttt ttctatatcc ccaagcttat ttatatatgc atcaatagca taccatattg
    13381 aaatcaacag tttttcggca acttctgttt ttactgatgt gctttgacca tttgtatatt
    13441 ttttaatcaa atccataagt attagagata tttcttgttg tatattatat acttctcttt
    13501 catctatcac tttacaattt agacattcct taagtagaga tatggaatat tggttcttta
    13561 ttaaattatt ttcgtttata ttaatattac ttaaattatt taacatacaa aatctctcct
    13621 atcatatatt aaacttctcc aaatttaaat ttctttgcaa attcgtctgc ttctcctctt
    13681 acattgtcca tattgcctga aaatttttca taaaattcac ccagtatttc tataatctca
    13741 tcatcacata tcttgtcatc agtttcattt tttaaataat aaaaaatttc ttgcatatca
    13801 ttaatagcct ctaaataatt gtccacattt gtatattgtg atgtatatat attttctatt
    13861 aactgttttg taacttttat atctaattct attcttccat acccctttaa agtattatct
    13921 ctcgaattta ttatatcttt tacatcagat tcagaaagag ctagaccata atctccagat
    13981 tctatattag tttcaagcag ttcatttctt tctatttcct ttaaattctg atttatagaa
    14041 ctcaaaataa taggtgatat catttttaaa ttctcctttt ttattttttc cattgacttt
    14101 ttttgttttt tatgctatac ttaaattaat ttatattatt ttaccaacca cttataatag
    14161 tagtgttaaa agtggttttt gtcaactcaa aattactatc tcaattaaac ttttcaattt
    14221 tctcaacaaa aaaataccat catttagata tataactttc tataacaact tttaattcag
    14281 tagttattta ctaaaatcta aacaatgata tatttattaa ttttatttat cttttttggc
    14341 tctacttcta cttttcttta catttgagca atttctacaa gaaggtgaac agtattgaga
    14401 tttagaattt gtaggaatat aaattttacc acagtacttg catttattta aagaagaagg
    14461 ttcacttgta attgcaaagg catagatagt ctcaatagct gttttcaaag aatcaaactc
    14521 ccatgcaata attggtttat ctaattggct tatagaaaat cctatctttt gaggattaaa
    14581 ccctccagac ataatagtaa cactttcagt taatagatta gatgtatttt tatatacaca
    14641 aatttgatta aaatgcttac acatatcttt agcaaaactt acaatccaat ctagttgttc
    14701 agtgtagaac tttgaaaata ctaaatctaa aatcaatggt ctctttccaa aaaactttgg
    14761 agattcttct ctctttgata catctatacc ccttctatac tttttaaaat acacatcttc
    14821 atcacttgca aatggtataa acatgtttac atattcatct gtactcaata tattctcttt
    14881 tgtaatataa ttcttttcaa tcatcagtac tctttcttct ccaactatat ctctattata
    14941 tacacttgaa tatattaaac ccaataatcc atactttttt acaaattcaa gtacttcttt
    15001 ctttagtttc atttcttttg tctcttttga tatatcttgt aatgcctctt gtcctatgcg
    15061 aattatatct actattatat catctgctac atcaaatggg ttatacatgg taaacacagc
    15121 atcttctgtt ggaactatat ataattcatc ttcttcagta attttatatt cataatcact
    15181 atacctaatc caatcacttg caaaattccc tattaattct atttttttat tcataataat
    15241 cattcacacc tttcaatgta atatataatt tcatattact attacacttt tagtttattc
    15301 catattttct acactgtcaa ctattctttt tatattttta gcatattaaa atttataata
    15361 gcctttataa tggatttcta gagctaaatt tcttgtttat tgatgactaa ttcataatta
    15421 aatttataaa aactatttac atataaaatt aatctcttta taattgatat gtaatatatt
    15481 tttacactta catattacat atcaattata aagagaggta tctaaaatca tggatagaaa
    15541 acgtgaatta aaacaattat acaaagaaat gaaatttgac acaggtgtat tcataataaa
    15601 aaatgatatt actaaaaaga tttaccttgg aaaaagtaat gacataaaaa gcaaatttaa
    15661 ctctctcaaa tttcagcttg gtgctggctc atgtatgata aaagaattaa ataatgaatg
    15721 gaaaaaatat ggtgaaaaag catttacatt tgaagtttta gaacttataa aacatgatga
    15781 taacaaaaca gagaaagatt atttagaaga actagatatt ttagagatgg tttggttaga
    15841 aaaattaaag gaagataata cttatgaaat ttattccata taaaaaatca tataatataa
    15901 gaaaaggaga tagtatatca aaatagacat ttagttcata agatactttt ttatataaaa
    15961 taaagtatct tttcataatt ttaacaatga aaaagaggct tatctccatt tgatagcctc
    16021 ttcccatata ttttatacca ttataaaatg aacaatctac taattttcaa tgtgtatatt
    16081 cattttacta ataacactcc attacttctt tacagcatcc aaacattgct ctataatact
    16141 cttatcactt atacctattg ctgttacttt actagctcca cttaatatac tcttatcact
    16201 tttatttgaa actaaaacaa caggagcatt ttttgcaagt ggtgatgcaa ctaaagcata
    16261 aactaaatcg tctccacttg ttatatagaa ctcttttgct ccattataga acttttcaat
    16321 aacattttta ttagtttgat atctatcaac accaccaatt ctagttgctc tatatctgct
    16381 cactatgtaa tcacttaatg tagactctcc tccaatagca tatactggaa caggagaaac
    16441 tccaaaccat gggtctaagt ctgtgtcttc ttctgctgaa ggtatatctt ttgtcaatat
    16501 tataggagct ccatctctat aagcaacaga tgcaacactt actgcatcag cttcatcctt
    16561 aaatccattt actaaaaaca tcttatcact gtttttaatt ttttctattt cacctgcaat
    16621 tttataactt gtatctactc tatctacacc atcaattctt ataacttgca ttcttctcat
    16681 aagtaacatt ttttctacat ctttacttat agaattttct ccacctatta catatatctt
    16741 agtaactcta ttcagtcttt gaagcgttgc ttctggtata tcatccttat tagttaataa
    16801 aatagctgca ttatttactc ctgcaagacc acttgcagca atcccatcta ccattgtaga
    16861 atctgcattg ataagaactg ctgttgtaaa atcttgctta tctgaaataa gcccagctgt
    16921 ttcatattta tctactcctt gaatattttc aatcttatct agtgcgttta ttgatgttgg
    16981 aaaactaaca cttaacagta atgctgaaat acctaacgcc aatacctttt tactaatcct
    17041 cataaattac ctccctaaaa caaatatatt tagtattagt ataatataaa atcatttaaa
    17101 aatagtgtaa aaatagttac acttttttat aatttaaata attttagatt aaacacaatt
    17161 catccattaa aataacttca tttattctat atgctaagcc atcccacgtt ttttttgaca
    17221 tatctacaga tgttttctta tttgagttat tcaataaaga ttgtatctta gaatctattg
    17281 cattatacaa attattttca aaatcatata actcttcatc ttttggaata ccttctttct
    17341 ccataaaagg tagagaaaca tattctatct ttccagaatt attaatctca ctaccaatcc
    17401 actctttaac accaagaata tcagtagtga ttacatctgt gccacaagat aaagcttcta
    17461 aaactacaac tggtaatccc tcataaaatg atggtaacac aaatatttgt gacctgttga
    17521 atagctcagc taaatctctc tgttctaact ttcctaggaa attcatttta aatggagact
    17581 tacttgccag tttaactatc tcattatatg attcgatatc gcttccagta cctgcaaaat
    17641 tgacttctat taaatcttta ctatatttta atttagatat agcttttata aatggaatca
    17701 gtccctttga tttacatatc ttacctgcaa atactatttt tattttatca tcctttattt
    17761 tataattttt attataaaat atatcatcat tataaccact accaattaca actactttac
    17821 tttgacttac tccaaaagtc tttataatat catatttctg attttcatga agagcaaata
    17881 ttaaatctaa tttccttata tttgctatta tgtattcttt ctctaaatca atggtattta
    17941 actgtcttaa acaagttcca tgacaaatag acataacctt tatatctttt accatttccc
    18001 taacaaatgc agttaaaagg tacaaatgat gacatattat aagttctggt ttaaaatcat
    18061 tcatagcctt atctatattt actttaaatt gatatttcaa tctatttatc atgtctatat
    18121 ttaaatcttt atatcttgta ctttcatatg gcatagaatc actcatacct acaacaggaa
    18181 aatttaactc cccacaatta tactttactg gataaaaact tacttcactt ggaaaacaat
    18241 ttacatcatc atttacatct atacctgcaa ttacagcttg cttatgaccg attttttcga
    18301 atccttttat cataccagac aaatatatac cactacctgt actatttggt ttttgtgtta
    18361 ttatatgaag aattttcact tttcacttgc ctcctttcta aaattaaata gttttataca
    18421 gtctaatcat tatatatatc taaatataaa caagatgaat catcttttac tttaaatctt
    18481 ggcactttaa ccgctttata gtcattatct tcaaaatccc ttactttatt atgtatatat
    18541 cctataccaa atttttctgc tatacgtatc aattcttctt cttttattag attatatcta
    18601 tcacatgcac ttgtaaaacc atcactcgtt agcatcaatt gaaaatcttt ttttatttca
    18661 atgtatccat atatagaatt ttccacagct tcctcatcaa aatccaatat ccaatagccc
    18721 tcttttgtat ttttcttcaa tctattgctt actattgtat ccataacact agttctaatc
    18781 tcatcaaatg ccatctcttc caagttagaa agtttactca tttcatcaaa tacagtatta
    18841 tcaaatttac aaatactcct atcttttata actttggaat ctccattttt aataaacaaa
    18901 gcacaatctc ctaataaaaa atactctact ttattttcat gaaattttac aacaactata
    18961 gaactagatg gtaaatctaa tttttcaacc ttaaacccat ttaatcttag ttcatactct
    19021 ttttttactt ctattattcc agtattaata atactcttta atgattcttc tttatttata
    19081 tttttatata aatacctatt ccaccactga acataccacc ttgcgtcact ttcagttgac
    19141 actaaatttt ttccattaag tcctgtagca ccatctaaaa cccatgcccc aaatggattt
    19201 ataccgacta catcttcatt aaatttagct gttccttgat tacataatag gctatagttt
    19261 cttatattta aagccatatc aacctccaaa ttgaatatct aaaaataaag ttctatttaa
    19321 aattatacag caaataaaaa aatctacaac aaaacttgtt gtagattttt acttaatcta
    19381 tattatcttt atactttcag tattttaatg atggaataat ctattcttag taaatgccat
    19441 gactatgtta tacttattac aagttaatat aacattatca tcacgaatag aaccacctgg
    19501 ttgtgctata aaacttaccc cacttctttt tgcacgttct atattatctc caaatgggaa
    19561 aaatgcatct gaaccaagtg ctactcctgt taaagtttta agccatgctc tttttccttc
    19621 tccagttaga ggctctggtt tttcttcaaa aaagttttgc catatgccat cagcaagcac
    19681 atccatataa tcatcagaaa gatatacatc aatagtgtta tctctatctg gacgacctat
    19741 atccttttta aattttaagt ttaatacttt aggatgttga cgaagatacc aagtatcagc
    19801 tttgttacct gcaagtctag tacaatgaat acgtgattgt tgacctgcac ctacccctat
    19861 tacttgtcca tctttagcat aacaaacaga atttgattgt gtatatttta atgtaataag
    19921 agctataatt aaatcacgtt ttgcactatc tgtaaaaatt ttattatcag ttggaatatc
    19981 ttttaatagg tcttcattta ttaatatttc atttctacct tgttcaaaag taattccata
    20041 aacatccttt gtttcaatag gttcaggagt ataattttca tctattttta caatattata
    20101 gttgccttta cgctttcctt ttaatatttc aagggcttca tctgtatatc ctggagctat
    20161 tataccatca gaaacctctc ttgctataat agttgcagtc tccttatcac atacatctga
    20221 aagtgctata aaatcgccat aagaagacat tctatctgct cctcttgcca ttgcataagc
    20281 acaagctaat ggagaaagct ctaaatcatc aacaaaatat atttgcttta aagtatctga
    20341 taaaggtacc cctactgctg ctcctgctgg acttacatgt ttaaatgaag ttgctgctgg
    20401 aagacctgta gcttctttta attccttcac aagttgccaa ctattaaatg catctaaaaa
    20461 atttatgtat ccaggttttc catttaatac ctctatagga agttctccat tcttcatata
    20521 aatctttgat ggtttttgat ttggattaca gccatacttt aattctagtt ctcttgccat
    20581 atactcatcc ctctctataa taaattaatc ataagtttga ttaatcctat attaattaaa
    20641 ttattcgtac taataaaatt atatggcata tttatccatt aatcaactat attttttaaa
    20701 actcctattc cttcgacttt acattcaact atatcgccat gctttaaata tacttgagga
    20761 ttcatcccca tagcaacccc tgctggagtt ccagttgaaa ttatatctcc tggctttaga
    20821 gtcattcctc ttgataaatc atttattata gtaggtatat caaatataaa gtactctgta
    20881 ttagaatctt gtcttacttc tccatttact acactactta tatctaattt taaaggatgt
    20941 tcaaattctt ctttatgtac tatacaaggt cccatagatg tatgtgtgtc taagcttttt
    21001 cctctaaacc attgaacatg tttatcttga agtgctcttt cacttatatc atttactatt
    21061 gtatatccaa atatatattc ataagcatct tcttttgata tatttatacc ttctttacct
    21121 atgattactg caagttctga ttcatagtcc aacttctcta ccaaatctcc atgtaagctt
    21181 atcttatcat ccactccaat tattttgtct gctctcttag aaaaataaat tggataatct
    21241 ggcataacta cgttttttat aactccatct gggatttcat tgacatggtc tttatagttt
    21301 agacctaaac atataatatc ttttttaggt cttacaattg gggaacataa tttaacttct
    21361 tctaacttat aacactttgc gttttcaaat gaattgtttt ttattttttc taaatctcct
    21421 gtagatacgt tttcaataaa atcattcata tcagtatata atttacttaa tcctaaacta
    21481 ttaatttcat atatactatt tgtttctgaa ttaaatacac ctattttttc ttcattgcta
    21541 ctacaaaaag tcacaaattt catttttatt cctccattca ataagttgat taatattatt
    21601 taatttttat tagtttatat aaattgtaat tcataatatt tatatgtcaa tacatattta
    21661 tatattttaa aaatattcaa ataattttta ttaaa
//
LOCUS       DBGJFU010000065        21630 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.065, whole genome shotgun sequence.
ACCESSION   DBGJFU010000065 DBGJFU010000000
VERSION     DBGJFU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.065"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            247..1518
                     /gene="serS"
                     /locus_tag="ACOIAS_002227"
     CDS             247..1518
                     /gene="serS"
                     /locus_tag="ACOIAS_002227"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGZ2701935.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKNFNLDEVVKLDDERRKTLQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1886..3391
                     /locus_tag="ACOIAS_002228"
     CDS             1886..3391
                     /locus_tag="ACOIAS_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HGZ2701936.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3505..4203
                     /locus_tag="ACOIAS_002229"
     CDS             3505..4203
                     /locus_tag="ACOIAS_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2701937.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4488..5576
                     /locus_tag="ACOIAS_002230"
     CDS             4488..5576
                     /locus_tag="ACOIAS_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2701938.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            5815..7005
                     /locus_tag="ACOIAS_002231"
     CDS             5815..7005
                     /locus_tag="ACOIAS_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2701939.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            7096..8598
                     /locus_tag="ACOIAS_002232"
     CDS             7096..8598
                     /locus_tag="ACOIAS_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HGZ2701940.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            8670..9926
                     /gene="glyA"
                     /locus_tag="ACOIAS_002233"
     CDS             8670..9926
                     /gene="glyA"
                     /locus_tag="ACOIAS_002233"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGZ2701941.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            10098..12872
                     /locus_tag="ACOIAS_002234"
     CDS             10098..12872
                     /locus_tag="ACOIAS_002234"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HGZ2701942.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(12973..13122)
                     /locus_tag="ACOIAS_002235"
     CDS             complement(12973..13122)
                     /locus_tag="ACOIAS_002235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGZ2701943.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            13497..14675
                     /locus_tag="ACOIAS_002236"
     CDS             13497..14675
                     /locus_tag="ACOIAS_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HGZ2701944.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      14802..14887
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            15005..17923
                     /locus_tag="ACOIAS_002237"
     CDS             15005..17923
                     /locus_tag="ACOIAS_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HGZ2701945.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      18109..18194
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18294..18956
                     /gene="zmp1"
                     /locus_tag="ACOIAS_002238"
     CDS             18294..18956
                     /gene="zmp1"
                     /locus_tag="ACOIAS_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HGZ2701946.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYAEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(19078..20040)
                     /locus_tag="ACOIAS_002239"
     CDS             complement(19078..20040)
                     /locus_tag="ACOIAS_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2701947.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(20210..21400)
                     /locus_tag="ACOIAS_002240"
     CDS             complement(20210..21400)
                     /locus_tag="ACOIAS_002240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGZ2701948.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         8559 a         2497 c         3691 g         6883 t
ORIGIN      
        1 aattgattag caaaatagat ataattataa tgaagttaaa tctagcaata ttctcctaaa
       61 attaaatttc atttatttaa ttaaatataa tttgacaatg ggcaaaaaag tgagtaaaat
      121 ataaataaaa taaaaataac aggttcggat aaagaaaaag gtttcttaac ctcaagcata
      181 aagtatatta tctaagttat ttgagattaa gagataaatc caatcttagg tactgaggag
      241 gaaaaaatgc ttgatataaa aagaataaga gaaaatttag attgtataaa aaaagcaatg
      301 gaaagaaggg gcgaaaaaaa ctttaacctt gatgaagtag ttaagttaga tgatgaaaga
      361 agaaagactc ttcaagaagt tgaagttatg aaaaatgaat taaatacagc atctaaaaat
      421 ataccaaatc ttataaaaga aggtaaagat gtagaaaatg aaaagattaa attaaaagag
      481 ttatctgata aaattaaggt aatagaccaa aatcttaaag aagtagaaga taagatggaa
      541 tacctattaa tgagaatacc aaatgtacca catccagaag taccacaagg tgaaactgat
      601 gaagataatg ttgaagtaag aacttgggga aagactacta cctttgattt tgaatctaag
      661 gctcattggg aaattggaac agaattaggt atacttgatt ttgaaacagc tagtaaaata
      721 actggttcaa gatttactct ttacaaagga ttaggtgcta gattagagag agcattatta
      781 aatttttact tagacacgaa tacaaaagta aatggatata cagaagttat tccaccattt
      841 atggcaaata gaaatagttt tttaggtaca ggacagttac ctaagtttga agaagatatg
      901 tttaaaatag aaggtatgga ctattttatg attccaacat cagaagttcc tttaactaat
      961 atacatgcta atgaaatact taaatttgaa caattaccga taaattatac agcttatact
     1021 ccatgtttta ggtcagaagc tggttctgct ggaagagata caagaggttt agtaagacag
     1081 catcagttta acaaagtaga aatggttaaa atagttgcac cagaagaatc ttacaatgag
     1141 ttagaaaagc ttactaacaa tgcggaaact atgctacaat tattgaatct gccttataga
     1201 gtagtaaaga tatgtacagg tgatttagga tttacggctt cttttaaata tgatgtagaa
     1261 gtttggatgc caagttacaa taggtatgtt gagatttcat cttgttcaaa ctgcgaagat
     1321 ttccaagcta gaagagcagg aataagattt aaaagagata aagattctaa agctgaatat
     1381 gtacacacat taaatggctc tgggttagct attggaagaa gtgttgcagc tatattagaa
     1441 aattatcaac aagaagatgg ttctgtggtt gttcctgaag ttttaagacc atatatggga
     1501 gtttctgtta ttaaataaga gttaaattat gtatttttaa gtaatacaat aatatagagg
     1561 taataaaaaa taattaatat aaaagtgata caataatata gaagtaataa aaaaatagtt
     1621 aatataaaag tgatacaata atatagaagt aataaaaaat agttaatgta aaagtgatac
     1681 aataatatag aagtaataaa aaaatagtta atataaaagc gatacaataa taatagaagt
     1741 aataaaaaaa aataattaat ataaaaatgt gtacaaacta atgaatattg atatagtaag
     1801 ctatcatatg aaagagtatg tacacatttt ttttactaat ttttcacaat atgaacataa
     1861 ataatttaca ggaaaggaat cttttatgaa aattcaaaat actgagtggg gatatattga
     1921 atggaaacat acttatgatg aaaataatcc aaaacaagct atgaatatat acattgcagt
     1981 aactatgcct ggtaagaaac attttaatca tgttcattat ggtcaagagc agatgatata
     2041 tattttagag ggagagggtt tgtatattat aaatggagtt tggaagccat tttatcaagg
     2101 tatgattttt tatatagaat ctggtagcac acatgaaact ataaatacag gcgatagaga
     2161 aataaaagaa cttattgttt caaataatgt agatgatgta ggagagagtg aagttataga
     2221 tattaatcct aataattatt taaagaagac cttgataaat tatagtgaaa gtacattaaa
     2281 tttgtatgca gcagtagaat caattcgtgg tcaatttata gacccgttta aaattccatt
     2341 aattatatat gatgattcat ggaatatagt tttaaaaaat ccttattttc cacttttttg
     2401 ttttgaaaaa tgtaatccta tgaaatttcc tcaaaattgt gattgtatga atcaaaaaag
     2461 tagcaatcaa tttgtatgtg aatatgggat aactatatat aatataccaa tactgtacaa
     2521 aagcaatagc ataggtgtta taagaggagg gtatgtacta ttatctgatt tgaatttaga
     2581 tacagagcat aataatttat atgatattcc agagggagct gctagaagta taaaacgcct
     2641 cttgaaacaa atatctaaaa atataataaa tttttgctct tttaatgata taagaaagga
     2701 tttacaagaa aaagagaaga ctatagcaag aacatatcat tatggagagc aacttgaaat
     2761 gaatttaaaa gtagctcaag atatggttac taatttaaga ataaatcatc actttttatt
     2821 taatacatta aattcaatgg caagcatagc actagatgat ggtagttatg atttgtatag
     2881 tgcaataata gatttatcaa gaatgtttag atatacaatg aggtctgatt taagatttgt
     2941 agaattagaa tctgaaatat tatacattaa aaattatttg aatttacaga aattacgtta
     3001 tggagatgca cttaaagtaa aatatcttat tccagagaag ttatataacc tttctgtgcc
     3061 ttttaacttt attcaaccta ttgtagagaa tgcatttaca catggattta gagatattga
     3121 cacagaaaag cgaattgaaa taattgcaag attagattct cagtatgcca ttatagagat
     3181 tcataataat ggaactatct tagatggaaa taatatagac aaaataaagg caggtattcg
     3241 tagtaataat ggtcatggtt tgtctttaat atatacaaaa ttcacatctg cttatggaaa
     3301 taattttgat atggatataa agtcgagtga taatgaagga acttacataa tgattaaaat
     3361 tcctattgaa aattataagg agtatgtata gaacttgtga aaaattaata agttttataa
     3421 atgttaagaa atatattaga tgtttttatg ttgttttata tatcttactt ttttatatta
     3481 ttaagctaaa agggaggtat tctcatgaga agagcactaa taatagatga tgaagaagct
     3541 gttctaaaaa taataacaca ttttatagaa atgaaagata tgccaataaa aattgttggt
     3601 aaagcaacat ctggtgatga agcagtagat aaaatcattg gtttagagcc agacatagtt
     3661 tttatagata ttcaaatgcc aatatacaac gggttagaag taatagaaaa aacatctcat
     3721 ttatctaaaa aaataaactt tgtagtaata actgcattta actattttga atatgctcaa
     3781 aaagctttga gattaaatgt aaaagatata ctccttaaac cattggatat gaaagaattt
     3841 actaagtctg tagaaaaaat tattggatat caatatacca ataatgattt attaaatgag
     3901 atactggaat atataaatct aaactatagc aatgacttaa agttaaatga ttgtgctaga
     3961 ttatttttaa ccaatccatc taatatatct aggattttta aaagtaattt aaatacaact
     4021 tttatatcat atctaaatca tataagaata gaaaaatcta ttgagttact tgaaaatact
     4081 acaaaaagta taaatgagat tgcagaaata gttggatata atagcctaaa taacttttat
     4141 aaaaacttta agatagaaaa aggaatgact ccaaaagttt ataaactgaa cactaaaaat
     4201 tgaaattgat aaataaaaag tcaatataga taattgtttg agtgtgtctg aataagtata
     4261 ctttaattat gataaaaatt aaagcgcttt aaacatattt aataatatta aaccttataa
     4321 aagctgtacc tatttatata aaaaaatata tataaataaa aagaaaaaga tatacaaaat
     4381 aaaaaaagtg aattaaatac tgtttcaaaa agacagaatt ttcatttaat ttactgagag
     4441 tttatatttc ttaaaggaag gtaactttat aatggaggta atttaatatg tcagacaaag
     4501 tgaatatgaa aaatattatc tgtattgcag gcgcttatct agcaacagca attggttctg
     4561 ggtttgctac tggtcaagaa attctacaat tttttgcctc acaagggtat atgggtttaa
     4621 ttggagcatt aatatcaatg gtattgtttt catttcttgg tgctgaagtt ataactagag
     4681 gtagagaatt aaaacttaaa gaaccaacaa gaatttacac tttttattgt gggaagattt
     4741 taggaaaatt ttacgaatgg tttagtccaa tatttttatt tgggattctt atagtaatga
     4801 tttcaggtgc aggctcaact ttaactgaat actatggtat aaatccttac ttaggaagag
     4861 tattgatggc tgttgctact ttaattacag tgtctatggg tttagaaaaa ttagtaagca
     4921 tacttggacg tattggacca gtgatagcag tatttacaat tgctgttgga ttaataagct
     4981 tgtttaagca ttttgacggt ttatctatgg ttggagaagc tatgaaaaat attgaagtta
     5041 caaaagcatc agaaagtcca atcgtttctg gtgtaatata taatacatta aatattattg
     5101 taatgattgt ttttttaaca ggaatgggag cttctattga aagtaaaaag gatgcttttt
     5161 ggggaggttt attaggtgga atagtgttta tgactgctgg tatagttatg tatttggcta
     5221 tgatttcaga tataggaaat ttgtatatga aagaaatacc atcactttat ttagcagata
     5281 atatatctcc gattgtaggt gtatgttttt cagttgcatt aatattaggt atctatacaa
     5341 cagctgttcc acttttatgg tctgtaacca atagaattgt tgaagatgaa catcctaaat
     5401 ttaaattggt aacaacagta atagctattt tggcttgtat aggaggtttt ttaccgtttg
     5461 gtaaattagt aaacatttta tatccatata caggttatat ggggctttta attttagtct
     5521 gtatggtgta taaagcaatt acatgtagaa aaaaatcaac acaaagtcag gaatagagcc
     5581 acttattaat agtttttatt taaaaaaatt ttttatataa tccgaattta aaaagtcaaa
     5641 atagttaaat aaattgcaaa acatgataat atatttaatt aaaataatat agtaaaatat
     5701 aaatataaac taaaattaaa tttataaaaa atatctttat aaagattatt aacttatata
     5761 atttgtagtt aaattataaa aaataaaaaa ataaaatttt ggagaggtgt tagtatgcat
     5821 gaacttattt taatctcaga aaagtataaa gaagatattt tagatttttt tacagatatt
     5881 catatgcacc ccgaattaag ttttaaagaa tttaggacta caaaagctat aaaagattta
     5941 ttagtgagtt tagacataga aatattagat ttaggaatgg aaacaggcgt tgttggatta
     6001 ttaaaaggga agtatgatgg tcccacagta gcactaagag gagatattga tgcgttgcca
     6061 atatatgaag aggttgatat taagtataaa tccagatatg atggtataat gcatgcatgt
     6121 gggcatgata tacacacgtc ttgccttgta ggatgtgcat atgtattgtc tcatattaga
     6181 gatagtcttc atggaaatgt aaagtttata tttcaaccag ccgaagaagt caataaaggt
     6241 gcgaagatgt tagtggaacg aggagtaatg gaaaatccaa aagttgatgc tatatttgga
     6301 cttcataatc atccagatat accttgtgga aaaataggtg ttaagttagg tgggttaatg
     6361 gcagcagtag acactattaa gatagaagta aatggatttg gaggtcacgg aggtatacca
     6421 aacagaacaa tagaccctat agtagcatca agtgcaatta taatgggtat tcaaaccata
     6481 gtaagcagaa acataagtcc attagaatca gctgttatat caataggtac tataaatgga
     6541 gggactgcaa ataatgtaat ttcagaaaag gtagatatga caggcacatg tagaagcttt
     6601 agtaatgaag tacgaaagaa aatatcagaa aatttagaaa atatagtttg tgagatagct
     6661 cgtggatatc aagcaactgc aaagctagat tatttatttg atttaccagc tgttataaat
     6721 agtaaagaga tgtacgcaat agcttgtaag tctgtgtgtg atttgtattc agaagatgct
     6781 atagttgatc caataccttc tacaggaggg gaagattttt ctatattcat ggaaaaagcc
     6841 ccaggatttt tttactggtt aggggtaggc aacaaagaac aagattgtat atatcagtgg
     6901 cataatccta aatttaaagc tgataaaaat tctatattgg taggtacaaa tgtactatgt
     6961 cagtctgtta taaattatat ggataaatta aaaaataaaa tttaagatag agcattctgg
     7021 agataaagtt tatgaggggg tattagtttg aggaaaatta attcacagga aaaaaacaaa
     7081 gatagtggat tattgatgag ggcactaaat gttgtagaga gagcaggaaa taaacttcca
     7141 gacccagtaa ccatattttt attactatgt ataattgttg taatattgtc agcagttata
     7201 tctaatttag gagtagaaga aatacatcct tctacaaaag aagttgtaaa ggtagtaaat
     7261 ctactagaga aagagcagat tcaatcatat ttaggtagca tagttacaaa ctttcagtca
     7321 tttgctcctt taggattagt actggttaca atgcttggag caggtgttgc tgagaaatct
     7381 ggatttatgg aagtgttaat gaaaaagggt ataagtaaag ttccacaaaa acttgtaact
     7441 gtagcaatcg tatttgcagg tatgttgtca catacagcag cagatgtagg atttataata
     7501 ttaccaccac ttgcagcact tgtgttttta ggaattggaa gacatcctct tgtaggtatg
     7561 tttgcagctt ttgcaggtgt tgcaggggga tttgcagcaa atgttatgct tagtactaca
     7621 gatgtattgt tagctggatt tacaatacca gcagctcaaa tgatggaccc atcttaccaa
     7681 ggcaatccag caatgaatct ttattttgct tttatatcag cgatagtttt aacttttgca
     7741 ggagcatttg taactgaaaa atttatagct cctcgtttta caaaatataa tgattcaaca
     7801 aatgataagg atatacagga attgagtgaa cttgaaaata agggtataaa atacgcttta
     7861 ttatcattgt tagttgtagt agtagtgata gttgccttat gtataggtga caatgcattt
     7921 atgaaagatc cagagactgg ttctatacta tctagtaatg cacctttaat gaaaggtata
     7981 gttcctataa tcactattat atttttaact ccaggattag tgtatggaaa agtaagtaag
     8041 aagataaaaa gtgacaaaga tttagtatct atgatgggta gtagtatgag tgatatgggt
     8101 ggatatatag tcctagcatt tatagcatcc cagtttatta atttatttaa tcttagtaac
     8161 ttaggaacaa ttttatcaat aacaggagca aaattactag cagagtcggg aatacctagc
     8221 tatggattga taataggatt tatattgcta tctggattta tcaatttatt tgtaggaagt
     8281 gcttctgcaa aatgggcgat tttagcacct atatttgttc ccatgtttat gctactagac
     8341 tttaatccag cattaactca aatagcttat cgtattggag atgcttcaac aaatccaata
     8401 agtccacttt ttccgtattt tccagttata ttggcctttg ccagaagata tgataaagat
     8461 ataggtatag gtactgtcat atcgaatatg attccatact cagtggtatt tacattaata
     8521 gaaataataa tattgttact attcatgggt attggcatac cattaggacc tggtggagga
     8581 atatcttacg ttttatagac cgttataata aatttatcaa tgaagattaa actaataaaa
     8641 tatattcttt aattaagagg aggattatta tgttaaaaac aacaaaaatt tcagacccag
     8701 agttatacaa gattgtagct gatgagttgg ttagacaaga acacaatatt gagatgatag
     8761 cttctgaaag tacagcacca acagaagtac ttgaattaag tggatgtgta tttacaaata
     8821 aaacagaaga aggctatcca ggggctcgtt ttcaagcagg ttctgaagaa gcagataaac
     8881 ttgaaaccct agcaataaaa cgtgcaaaag aagtatttgg agcagaacat gtaaacgtac
     8941 aaccgtattc aggttctaca gctaactatt gtgtatattc atcaatatta aaaccaaatg
     9001 acactgtttt aagtatgcgt cttgaccaag gtggacactt gacacatggt agcgcagtta
     9061 attttttaca tgacatttat aaatatgaat tttatggggt agacccaaat actggacgta
     9121 tagattatga tgctcttgaa gcaaaggcaa aagaatgtag accaaaactt ataattgcag
     9181 gggcaagttc atatccaaga ttaatagatt atgaaagaat atcaaaggtt gcaaaagaag
     9241 taggagctta ctttatggta gatatggcac atgttgcagg attagtcgca gcgaaagtaa
     9301 tacctagtcc tgtaccttat gcagattttg tttcttcatc tacgacaaag actttctgtg
     9361 gaccaagaag tggtattgta ctttgtaaag cagaacatgc aaagaaactc gataaaggtg
     9421 tttttccagg aacattaggg tctatacatc ttaatacagt tgcagcaaaa gccttttcac
     9481 tattatattt aagtacagat aaatttaaaa agataatgga gcaagttgta gtaaatgctc
     9541 agactctagc ttctgaactg atttcacatg gttttagtat tgtaagtgga ggtactgata
     9601 atcatatagt aatggttgac ttaagaagta agaatcttac aggaaaacaa tttgaaaaag
     9661 ctcttgaata tgtaggaata acagttaata aaaatgttat acctgacgac ccacaatcac
     9721 catttgtaac tagtggtgtt cgtataggtc ttacatcaat atctcaaaga ggtctgaaag
     9781 aaaaagaagt tatacagata gcagggataa tgaataaggt agcagaaaat attgataata
     9841 aagaagtatt agatgaatgt aaagcagagg cacaagaact tatatctaag tttccattat
     9901 atccagaagg gtattttgaa gattaaatat tttgatttgt atagctcaca atttataaag
     9961 cctgtaacat aaaatttaat aatatataac cactataagg tgttgattaa tcaacacctt
    10021 attttttttg aaaagtaata tacaagctat cataagcata taattcctta tagataatta
    10081 acaaggggga taaagttatg aggtattata ataaacctac caaagaagta ttaaaatact
    10141 taaaaacaaa tccagaaatt ggacttgatg ataatgaggt agaagaaagg aagcttagat
    10201 atggattaaa tgaatttact ataaaagaag gtagaacatt ttgggacgag ttgggagaaa
    10261 gtttgacaga acctatgata ttaattctca taggagcagc agtaataagt tcttttgtgg
    10321 gagaattaca tgacgcatta ggtattttag gggctatttt tattggaatt tctataggca
    10381 taataactga aggaaaatct aaaaaagcag ctcatgccct gtcaaaatta acagaaaata
    10441 tagaagtaaa ggtgcttaga aatgggaaga taattaaaat atcaaaaaat gatttagtac
    10501 caggagatat tgtatatata gaaactgggg atatgatacc agcagatgga agacttatac
    10561 agtctataaa tttgaaactt agagaagata tgctaacagg agaatctgat gatgtagcta
    10621 agaatgctga tgctgtatta gatatggaag tagtttattc taaaacagaa ataatcgaac
    10681 aggatgcaat acctgcaaag caagtcaata tggtatttgg agggactctt gtagcatatg
    10741 gtaggggcat aatggttgtc actcatactg gtgataagac agagatgggt aagatagcac
    10801 agaatttatc aaatgaagac cagcaaactc cacttcaaat taaattaggg aagttgggtg
    10861 ctaaaatagc gggaatatca ggcgtaatag ctactttatt atgtatgttt atgattatac
    10921 agatgcagag aaaaggtatg ttaattttag atacaagcag tgtattatct ttcttacaat
    10981 cattagaacc tgctaaaaat gcatatatgg tttgtattgc attaatagtt gcaactgttc
    11041 cagaaggttt gccaactatg ataaatatta ccttagctat aacaatgcaa aagatggcaa
    11101 agataaatgc attagtaaca aagaaagaag cctgtgaaac tataggttct gtttctgtaa
    11161 tatgttccga caaaacaggg actttaacac aaaacaaaat gatggtagag gtagcatatg
    11221 ttgatggtaa atatataagt ggaggagagt atcaaagtaa tagctatttt gaacaaaact
    11281 gtatagtaaa ttcaactgca gatatagaaa aagaagataa ttcatttaaa tatataggaa
    11341 gcgcaacaga gtgtgcatta cttttatatc ataatgataa gaactataat gaaatgagaa
    11401 aacaaacgta tttgatatca caaataccat ttagctcaga agaaaagaag atgtcaacat
    11461 taattcgtca agaggattca gatatattac tttcaaaagg tgctccagaa gtattattaa
    11521 aaaaatgttc ttatgtacaa caaggaaaaa atattgttcc aataactcca aaagtagaaa
    11581 aaagtatttt agatgagatt aagaagttac aaattaaatc tatgagaaca ttaggatttg
    11641 cttataaaaa aatgagtaat tctaaaacag aagttgcaat gacttcagaa ggtgaattga
    11701 atttaatagg aaattcaaga agttatatga aggaagataa tttagttttt agtggatttg
    11761 taggaatagt ggacccattg agagaagggg tcaaagattc cattgataaa gcatttaatg
    11821 caggagtaga tgtaaaaatg cttacaggag ataatataaa tacagcaaca gctattggaa
    11881 atgaattagg attattaaat gatgggaaaa aggcagttga agcaacatat atagatgtat
    11941 tgacagataa agaattgaga gaagaaatca aaggaatatc tattgttgca agaagtaaac
    12001 cagatacaaa aatgagaata gtttcagcac ttcaaaaaag tggcgaagtt gtagctgtaa
    12061 caggtgatgg tataaatgat gccccagcac ttagtcaagc agatgttgga attgcaatgg
    12121 gtatttcagg aacagaagta tctaaaaatg cagcagatat aatattgact gatgatagct
    12181 ttagtactat agtagaggga ataaaatggg gaagaggtat atatgagaac tttcaaagat
    12241 ttatacagtt tcagcttact gtaaatatag tagcatttat aattgctata atatctcaac
    12301 ttactggtaa ggatatgcca tttactacaa tacaattatt atgggtaaat ataataatgg
    12361 atggtcctcc agccctagca ttaggattag aacctgttag agattatgta ttgaaaagaa
    12421 aaccaataaa tagacattct ggaataatag ctaggtctat gtttgtaaat attattataa
    12481 atgctattct tataattaca attgtattta ctcaatcagc ttttaatata ttgggagcta
    12541 catcagaaga acaaggtact gttatatttt ctttatttgc attcagtgcc ctttttaatg
    12601 cattaaattg tagagagttt ggtttaaata gtacaatacc aaatttcttt aaaaataaac
    12661 tggcattgca aataatagta gttacaggga taattcaaat aatatttaca caagtgttcc
    12721 agagtttctt taattctgtt tctttggact ttgatatgtg gataaagata atactatttg
    12781 catccactat cctattgtca aatgaatttg taaagctaat tcttagaaca atgaaaaata
    12841 gtagaactat gaactttaat agtaaaaatt aattcctatg aaaccgcaaa gtactatata
    12901 ttgtaacttt gcggtttttg tttgtttaat gttgttttat attaccattt tcatatttaa
    12961 ttttttatta atctaaacac aatcttccat tgtaattgct tcatctgtgt caacacattc
    13021 aaagtcaata tctaattttt gtaatatttt gtatgcctct ttttgcaaag gagtatcaaa
    13081 ttcaataggt gattttgtgt aaatataact gataaaaaac atgatttatt tcattccttt
    13141 ccatttaaaa acttatagtt atagtgtatc ataaggttat aaaatataga taaatatagt
    13201 ataaatttaa aatatgtaca gacaaaacaa aaaaatcata ttctaagtaa tacttgggaa
    13261 aaatgatatt atcataataa ataatctttg taaaaactta tatagaatct attaaacaga
    13321 cactaagcca taaaaacttt actaaatata aaattcgtat ctaaatagat atatagaata
    13381 atattttact gaagtagagt gatttttaag aattttatat ataaattatg ttatcataaa
    13441 taaagatata gattataaaa tttgttaata aaataaaggg cagggaggta agtttcatgt
    13501 tttccaaaat cctagtcaaa acatttatca gagatagcga aaacgttcaa aatacagatg
    13561 ttagaaataa gtatggttat gtagctggtg tagtaggtat attatccaat ttacttttat
    13621 ttgtgataaa ggtattcata ggaatgctca catctagtat agctataatg gctgatgcat
    13681 ttaataatct ttctgatatg gcatcttcag caataactat gataggtttt aaattagcaa
    13741 gtaagccagc tgataaagaa cacccttttg gtcatggaag aatagagtat ttatcagcat
    13801 taatagtagc atttatggtg atgttggttg gacttcaatt tgtaaagtca tctatagaaa
    13861 ggatagtaaa tccaattcct gtaaaatttg aagtaatacc acttatactt ttaattgcat
    13921 ctataatgat taaaatctgg ttgagtcgtt ttaataaatt tatgggaaat aaaattgatt
    13981 cttcagcctt aaaagcagtt tcacttgatg ctttaggaga tgtatttact tcaagctgtg
    14041 tagttatatc atttattgta gctagattta ctaattttcc tatagatgga tatgttggta
    14101 tcgtagtatc attagtgata ctttatgctg gtttctctct tgtaaaagat actataaatc
    14161 ctcttttagg ggaagcacca gatgaagaaa tggtaaattc tattatagag ctattgttat
    14221 cttataaata tataataggt acacatgatt taattattca taactatgga gttggtagat
    14281 gtattgcatc tatacatgca gaaattccat ccaacataga tataatggaa atacatgaaa
    14341 ttatagatac tgcagagaga gaaatatctg aaaaattaga tatatatcta gtaattcaca
    14401 tggacccaat atgtcttgaa gataaagaag ttatgtcagc caaaaaagaa cttgaagaaa
    14461 tacttaagaa aaatagtctt gtaaaatcta tgcatgattt tagaatagtt ggaaaaggaa
    14521 gcaagaagaa tttaatattt gatatagtag ttaatccttc cgaattttcc aaaaacatgt
    14581 cagaagatga cttaaaagaa gatataacaa aattagttaa ggagataaat cctgaatata
    14641 attgtgtaat tgttgtagat aaggatttta tttaattatt aattaatctc atcataaggt
    14701 aaaaaatgta aaaaggatag acaatagtat agaggcataa tataatagaa gtatataatt
    14761 gtatttttaa tataaataaa gcaattttaa ttgtttttat gaaatattat agagatgttg
    14821 aagtatattc tattattggg caccttatgg atatactgag tcagtggtgc aaccggctat
    14881 gaatataaat ttatttattt tcatagcttt ttttgtataa ttttttagta aaaattactt
    14941 tatatacgat attaatggaa gattagatta taaattatag gtaacctaaa ggggtgagat
    15001 ttacatgaag aaaggaaata gaaaggcatt attaattagt ttaattatga ttttaagtat
    15061 ggtggtgtct acaatatatc caactgtatc ttatgcttca gaattaggag agaatagtca
    15121 gattcaaagt ggttcaacta attcatcaac tggagaggag aaggaaagcg acaataaaaa
    15181 gccagaacag actccagaga aagataaggc aacagataat aaaaaaccag aacagacccc
    15241 agaggaagaa aaaccaacag ataataaaaa accagaacag accccagaag aagataagtc
    15301 aacagataat aaaaagccag agcaaactcc agaaggagaa aagccaatag ataataaaaa
    15361 gccagagcag ataccagaag aagataagtc aacagataat aaaaagtcag aacaagcttt
    15421 agaagatgaa aaaccactag acaataaaaa tacagaaaaa actccagagg aagataatct
    15481 attagaagat gaaaatttat tgaaagtttt agaagaagaa cttaatgaag aaaatgaaga
    15541 ctatggattt gttgtaaaaa ttaataacaa tacaatagaa acggaatcaa tgaaaaaaat
    15601 atcatttaat ttaacttata ctccaacatc aaaaggaata caagcaggag attcaataac
    15661 ttttaaggtg ccagatgtat tcaataaagt taatttagat tatacaagtg aatgctttga
    15721 taaaacagaa agtaatggag agtatacact tacatttaga gaattaccta atggccaaag
    15781 tgtaatgcaa ggtaaaatag gtttggaagc atatgttaaa aaggttgatg aagatactaa
    15841 tgcaaaaata catattgaaa ctactggaaa aatagaaagt ggaagtggag atatagatgt
    15901 agaaataaaa cctggagata aaacggatgt tccagatgct aaaggaacac ttaaaaagct
    15961 tgttgaagga aaaaaatcaa ctactgtatt tatgccagtt aaaaataaag atataaatta
    16021 cagtatacaa gtaaatgaaa agcaggaaga gttaaaagat attattttgt atgatgagct
    16081 tcctgaagga ttgacattga taaatggttc tgtaagtgta gtaacaagtg atggaaaaga
    16141 agttagtgat tttaatattg aacagagtaa aaattctatt tcagttaatt ttggaaatat
    16201 agataaatcg tatactgtta agtataaggc tagaatcagt gacaaaaacg caaaacatgg
    16261 taataaatat aagaatgtag ctaggattga atctgatggt aagaagatac aagaagatga
    16321 tgctactgta agtatatttg atagaggaga tgattatctt cttactaaag gacattcagg
    16381 agcaacaaat attactcaag ttggtcaagt aataaactat cagatttcaa taaatgatga
    16441 taaatcacca ataagtaatg ttgttataac agataatata ccagaaggca tgagattaac
    16501 tacttcagga gaagctgggc atgattttag agttgtagaa attccaatga atgggagctg
    16561 gactccttgg agtaaagaaa aaattgccaa taatatttca tataaagtag aagaaaaaag
    16621 aaatgaaagt gggcaagtag ataaagttat aactggattt acaattaatc tcagtaaaga
    16681 agaagtggaa tcaaagtttt ttatagctta tacattaaag gtaattagta ttgaagatag
    16741 ctatataaat agagctgttc ttgatgcaaa taatagtgaa attgataaaa atgatgagat
    16801 aaactttaag aaaaattctg gattaataag cgcaaaaaaa gaagttgata aaaaagtttt
    16861 aaatagtagt gataaccaga ttgttaaata caaaatcaat atgtcaactt atggagttta
    16921 tgatgcagga caagtaaatc ttttagatga agtaaactca gctctagaaa tatctaatat
    16981 aaaatattct gataatcttg aacttaaaaa agaagctgga gatggaaaaa atacaataag
    17041 attagtaaat aaatacgaat ttaaacagat aaaagaaggg gaacctgtgc aaagttgggt
    17101 tacatttgat gcagacttca caaatgtaaa agttggggag actatacaaa atgtggctca
    17161 gattaatggt tcaagtcctc caggagtaga gactactaag caaggttatg cttttgaagc
    17221 taaaaaagtg gatgctttag ataaaaatgt actttcaggt gctaagttca atttagaaga
    17281 tgcatttgga aatatagtag ttaaggattt agttagtgat gaagatggta taattcaatc
    17341 tagtataaaa aaaccaggaa catattattt agtggagata atggctccaa gtggatatga
    17401 aaaactgaag gataaaatta aggttgaaat tggtaataaa gatattggta gaatagtgga
    17461 tattggaaat atagaaaaca taagacaaga aaatccacca gtaaatccac cagcaccacc
    17521 aaatacagat gaaccaatag taaatccacc agtaccacca agcacagata aaccaaggaa
    17581 accatcaagt tcatctgata cagaggatac tatagttata aatccaccaa taccaccatc
    17641 agaagatata ataaatccgc caataccaga ggtattgaat ccgccagtac caccatcaga
    17701 agagatgata gaaactcctg ttaaacagat tattccaata ccagaagtag taaagccatc
    17761 agtgtcagaa gaaaaaaata ataaagttaa agatgatact ttggtaaatc caccagtacc
    17821 accaaaaaca ggggatagta ctacaattat tggagaaata cttttggtta taggagctat
    17881 tgtaggactt atcgtattaa gaagaaataa aaatacaaat tagaccctaa cattataaaa
    17941 aatataagtt atattaaaaa atatcttcta aaacaatatt taataatgaa aatattgtta
    18001 gaagatattt ttttttgaag atttatgtaa aaaaataata actttttaga aaaaagttcc
    18061 aaagggatat agacaattaa aaagatataa gctataatat agatataaaa ttaaatgagg
    18121 caaaactagg gaaacctagt cacgcaaagc tataggggct aaagtttagt aaaccatgcc
    18181 agccagttgc cgaaaagagt aaaaatttct ttttgtatat acaaagatac tcttgggtat
    18241 cttttttatt ttgaaattta ttactcccgg cctaactcaa aggagggaat aatatgagac
    18301 caagtaaaaa attattaata gctataatat caatattctt aataagttca gttccagtaa
    18361 gtgcacatgc agatagtact actatacaac aaaataaaga cacacttagt caaatagtag
    18421 tttttccaac tggaaattac gataaaaacg aagctaatgc tatggtaaat agattagcta
    18481 atatagatgg aaagtattta aatgcattaa agcaaaataa cttaaaaata aaattattaa
    18541 gtggaaagtt aacagatgaa aaagaatatg cttatttaaa aggtgtagtt cctaagggat
    18601 gggaaggtac tggaaaaact tgggatgatg ttccaggttt aggtggaagt acagtagctc
    18661 ttagaatagg atttagtaac aaaggtaaag ggcatgatgc aataaattta gaattacatg
    18721 aaacagcaca tgcaatagac catatagtat taaatgatat atctaaatca gcacaattca
    18781 aacaaatatt tgctaaagaa ggtcgttctt taggtaatgt aaactactta ggtgtatatg
    18841 cagaagaatt ttttgcagaa tcatttgcat attactattt aaatcaagat acaaatagta
    18901 agttaaaatc agcttgtcca caaacttata gctttttgca aaatttagct aaatagagtt
    18961 ttaataaatt taaaataaat ataaataaag agatgctatt ataatagcat ctcttttata
    19021 ttgcgtttaa atctaataaa ttttgtctta tgtataacta tgatagatta tcaattacta
    19081 ctccttatac aaggcttcaa cgtgtttatt atgcatttga gtaattatcg taggtataat
    19141 agaaattatt gaaccaatta ctatcaataa aattcctata accatgttta tagggataat
    19201 ttctttaagt attaataatg ctagtattgg agatattact ggtttaaaga agaatactaa
    19261 agatgtaaga ctagtagatg tagcttccat tgccataaag taaagcgcat aaccaacacc
    19321 tgtaacaaat acgaatacat aaatcataat gggcatattg tgtaaggtat atccactaaa
    19381 aatagggata tttgcaaata catgaagctt atttttattt aatatatctg cgataaaatt
    19441 aattttagtg agtaaaacca atatcaacat ttccaaacta ccaaatataa agctaaagca
    19501 ggttacaact attccaccaa attttaaggt ttttctttta ccaaatacag tatacattga
    19561 aaaaataata gcagaagata atgttaagaa tattccacta agagttagtt ttgtatgcaa
    19621 tggatttatt ataactataa ttccaagaac ttctaatata agtgaaatta cattgtgcct
    19681 atatattttt tccttcaaaa ttaagtaagc taaaagcatt acaaagactg gattacaact
    19741 gaacaaaaca gcaactacag atgcctttgt gtttagaaca gcaagttgat acaatgacat
    19801 acttactact acacacataa aacctaaaaa tgcaaagtat tttaagtctg attttgtaat
    19861 agaaagacta agagctctta atctttttac agcaaaaggt agtaagacta aaccgccaac
    19921 aaaaaatctt gataaagtta tttgtaaggg attaaagtca ttagtgacag tttttagact
    19981 aatttccatt gaactaaaaa ataaggtagt cagtattata taaacataac cttttttcaa
    20041 gtcaaacacc tccttgtaat ttatattagt actatagata agaatattca agcatattgg
    20101 aataatatgg tatgtataaa atatagtaca taaaattata aactacctta atatagaatg
    20161 taagtattct atactaaggt agttaattat ttgaattact ttgatttagt tattttaaat
    20221 tatataattt ggctaatttt tcaaatgctg gtaaagaact tttaacttta tcataagata
    20281 cacaatatga tactctaaaa tgacctggac aaccaaaaga agaacctggt actaataata
    20341 aattaaattg ttttgcatca ttgcagaatt ttttatcatc ttcaattgga gcttttggga
    20401 acaaatagaa tgtaccatta ggtttaacac aagaatatcc catatcgatt aaactattat
    20461 atagaaggtc aaaattcttt ttataaatat ttacatcaac ttctgcatct aaacttcttg
    20521 caataaccct ttgaaaaaga gaaggagcat ttacaaatcc aagtattcta tttgcaacat
    20581 ttaatgaaga cataacatca tcaaaatcat ccatttgccc attagcaaca atatatccta
    20641 tacgttcacc aggtagagat aatgatttac tataagagta tcctataaat gaatttctat
    20701 agtattttag tacacaagga actttggcac catcataaat tatttctcta tatggctcat
    20761 cagaaattag gtatatgctt gtattaaatt ctttttgttt tgaatccaat aattctccaa
    20821 gattttttaa taattcttca gaatatatga caccagtagg attatttgga gtgtttatta
    20881 ttaaagctct tgtttttgga gtaatagcat tttttagagc atctaaatct ggctcaaaag
    20941 tctctatatt tgtaggaact tctattaatt ttccatcata gttttctgta taatttttat
    21001 actcaccaaa atacggtgca aaagcaataa cttcatcacc aggatttaaa agagttttta
    21061 aaataatatt tagaccacca gcagcaccac aagtcataat taaattttct cttgtaagat
    21121 ttaatccatc ttttttattt atgtgttcag caatagcatc tcttacatcc tcatatcctg
    21181 agttgttcat atatccatga actaaatttg gagattcttc atttaaaata tcatttataa
    21241 ctgctttaat agtctctgga ggttcaacac ttggatttcc tatactaaaa tcaaaaacat
    21301 tttcctcacc ataaatatca gaaagtttct ttccttcttc aaacatagct cttataacag
    21361 aactatttgc aactaaagtt tgcattttat tagatatcat atgtaacact cctttttata
    21421 gatttatatt gcttatgtat atattaccat atttttactg aatttagaca attttcgaaa
    21481 aattacttgt ttgaaaatat aaacttttat ataaaatata tttatgtaca gtacatatta
    21541 aaaattccaa tttctaattt tatcatgatt attttatata atagtttttt aaagaattaa
    21601 taaaacatag atttagagaa taaaatattc
//
LOCUS       DBGJFU010000066        21233 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.066, whole genome shotgun sequence.
ACCESSION   DBGJFU010000066 DBGJFU010000000
VERSION     DBGJFU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.066"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(188..1579)
                     /locus_tag="ACOIAS_002241"
     CDS             complement(188..1579)
                     /locus_tag="ACOIAS_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGZ2701949.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1590..2810)
                     /locus_tag="ACOIAS_002242"
     CDS             complement(1590..2810)
                     /locus_tag="ACOIAS_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HGZ2701950.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2824..3612)
                     /locus_tag="ACOIAS_002243"
     CDS             complement(2824..3612)
                     /locus_tag="ACOIAS_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGZ2701951.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3629..4903)
                     /gene="larA"
                     /locus_tag="ACOIAS_002244"
     CDS             complement(3629..4903)
                     /gene="larA"
                     /locus_tag="ACOIAS_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HGZ2701952.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(5021..5734)
                     /locus_tag="ACOIAS_002245"
     CDS             complement(5021..5734)
                     /locus_tag="ACOIAS_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGZ2701953.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5859..7118
                     /locus_tag="ACOIAS_002246"
     CDS             5859..7118
                     /locus_tag="ACOIAS_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HGZ2701954.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7398..7964
                     /locus_tag="ACOIAS_002247"
     CDS             7398..7964
                     /locus_tag="ACOIAS_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2701955.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(8165..9370)
                     /locus_tag="ACOIAS_002248"
     CDS             complement(8165..9370)
                     /locus_tag="ACOIAS_002248"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGZ2701956.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9473..9579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9671..10039)
                     /locus_tag="ACOIAS_002249"
     CDS             complement(9671..10039)
                     /locus_tag="ACOIAS_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HGZ2701957.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(10091..11368)
                     /gene="obgE"
                     /locus_tag="ACOIAS_002250"
     CDS             complement(10091..11368)
                     /gene="obgE"
                     /locus_tag="ACOIAS_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HGZ2701958.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11479..11769)
                     /gene="rpmA"
                     /locus_tag="ACOIAS_002251"
     CDS             complement(11479..11769)
                     /gene="rpmA"
                     /locus_tag="ACOIAS_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGZ2701959.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11783..12100)
                     /locus_tag="ACOIAS_002252"
     CDS             complement(11783..12100)
                     /locus_tag="ACOIAS_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HGZ2701960.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(12115..12426)
                     /gene="rplU"
                     /locus_tag="ACOIAS_002253"
     CDS             complement(12115..12426)
                     /gene="rplU"
                     /locus_tag="ACOIAS_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGZ2701961.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12527..13948)
                     /locus_tag="ACOIAS_002254"
     CDS             complement(12527..13948)
                     /locus_tag="ACOIAS_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HGZ2701962.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13966..14667)
                     /locus_tag="ACOIAS_002255"
     CDS             complement(13966..14667)
                     /locus_tag="ACOIAS_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HGZ2701963.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14669..16513)
                     /locus_tag="ACOIAS_002256"
     CDS             complement(14669..16513)
                     /locus_tag="ACOIAS_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HGZ2701964.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16832..18025
                     /locus_tag="ACOIAS_002257"
     CDS             16832..18025
                     /locus_tag="ACOIAS_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HGZ2701965.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18237..19319)
                     /locus_tag="ACOIAS_002258"
     CDS             complement(18237..19319)
                     /locus_tag="ACOIAS_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HGZ2701966.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(19883..20674)
                     /locus_tag="ACOIAS_002259"
     CDS             complement(19883..20674)
                     /locus_tag="ACOIAS_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HGZ2701967.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20678..21007)
                     /locus_tag="ACOIAS_002260"
     CDS             complement(20678..21007)
                     /locus_tag="ACOIAS_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGZ2701968.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         6703 a         3827 c         2357 g         8346 t
ORIGIN      
        1 tttatgtaca catgctttta ggatgacaac atcaacttaa atattatgtt actttctaaa
       61 tttaagtaaa tattgccatc ctaaaaattt tacaatttag cttcagattt aaaagtaaag
      121 tttaaaatat tatatttaaa tctaatttaa tattttttgt tttgtaaata ttattaaatt
      181 aatatttcta atatattttc cctggattta aaatattttt agggtcaaaa gctaacttta
      241 tatttttaat taacatcata taagcatctg aatttgattc atgtaagtac tcttttttag
      301 catatcctat gccatgttca ccagaaactt gaccactaag ttctctagat tttttataca
      361 tatagtcaaa agtttctttt aatcttattt tccatgtatt atcatccata ccatctttta
      421 aaatatatat gtgtagattt ccatctcctg catgaccaaa actttttatt cttattttta
      481 atttgtcttg aagctcgtga gtgtatctta taaactctgc tatcttatct cttggaacaa
      541 cgacatcaca ttcatccatt tgtgtcgtag atgcctttat agcttctaaa aatgcacctc
      601 tggctgacca tatagaatcg tttctttctt gtgtatctga tataaataca tctaatgcac
      661 cattttctaa acataaatta gctacttttt catattcttt ttctatatct tcagtgctat
      721 ttccatcaaa tgtaagcaaa agataagcat ctgatgtatt atctggaaac tttttaccta
      781 aaaattcttc tgctgccaat atcacatctc tttccataaa ttctatagcc gttggtattg
      841 attttgattt tattatttta ggaacagttt ctattgccat tgataaatct ggaaatggta
      901 taagcaaact tatacttttc tttggtaaag gaagcaattt taatatagct ttagtaacta
      961 ttcctaaagt accttctgag cccactaata aatcctttat actatatcca gaactatttt
     1021 taaccacctt tccacctaca tttataattt ctccatttgg tagtacaact tcaagacctc
     1081 ttacataatc ccttgtaacg ccatatttta ctgctctcat accacctgca ttagtgttta
     1141 tattacctgc aattgtagca cttttttcac ctgggtcagg tgggtaaaat aaatctctat
     1201 cttgaacata ttgacctatt gtcatcaata aaacacctgg ttctactgtc aatgttaaat
     1261 tttcataatc aacttcaagt attttattca ttttacatag attaatcatt attccaccat
     1321 ttatggcaac agcagcacct acaagaccag taccttgacc tcttggtgtg actggtatat
     1381 tatttttata tgcgtatttc attattttag atacttgttc agtttcaagt acatttacta
     1441 aaacttcagg gtacttctca atccctccaa gttcatcatg tgaaaaatct tcatttatat
     1501 cacttccaac taatacattt tcttctccac aagtatctat taaaaaatct atatcttttt
     1561 tatcgattaa tttatacatt tctatccccc tatttagctg ctgatgccat atatttttga
     1621 ttattatctt tgttattttc tattttttca atcaatttag gaataatatc ataaatatca
     1681 cctattaaag ctaaatgtgc tacatcaagt ataggcgctt tatcatcttt atttattgct
     1741 actatatagt cagcaccctg catccctgca acaaattgaa cagctccaga tacaccacaa
     1801 gttattatca atttaggttt tacagttcta ccactaagac ctatttgttt tttagcatct
     1861 atccaacctg cttctattac aggtcttgtt cctgccactt gagcgtttaa cttatcagct
     1921 aatttatata tcatctccat atcttcttgt ttttttattg ctctacttgc cacaactatc
     1981 acttcagctt cttccagacc aacttcttta tttttttctt ttacactcag taccttaatt
     2041 ttagatttta gttctttttt ttctacttta cataaagtta tctttcctgt agtattctct
     2101 attttttctg gtgctgaaaa tattttatat cttacagtgg caaattgtgg tctattatta
     2161 ggtgtgttta tatgagccat tatatttcca ccaaatgctg gtcttatttg gtctaaatca
     2221 gtatttgatt gaatatcaag tattgtacaa tctgctgtaa gacctgttct aaatctagcg
     2281 gcaagtcttg gagctaatga cctacctaat gtagttccac caactaatac tatcgttggt
     2341 tttattttat ctataaagtt ttctattgct tttgaatatg gttctatcct aaaatcttta
     2401 aattcatctt catcatatac aaatacttca tccaccccat aagaaatcaa attactgcac
     2461 ttatctttta tgtcttttcc tacaaataca caatacacag gttgttttat ttttgatgcc
     2521 atctcacgag ctttacctat aagctcgtac gttacaggat gtatatttcc attatgatgt
     2581 tcaacatata ctgcaattcc cctccattca tctttattaa tttgtacttt actactctct
     2641 ataaattcaa atgcgccttt tggtccactt ttgacacaca acttgcacat cttacagcca
     2701 gcacttattt ctactatacc tgacttttct tctatagccc caaaaggaca tatttctaaa
     2761 attctcttta agtcagttat tttatcttga tttacaacta tctttgacat ttattactcc
     2821 cccttacacc aactttaatt cctctaattt atttacaagt gcaaaactta gttcatctgc
     2881 atttcctctt acaatatttt tatctgtatt tggctttgga ggaaatattc ttacaacttg
     2941 agtaggagaa ccattaagtc catagttatt ttcattttta tcttcaaagt catctaagct
     3001 atatacaggt atttctctat ctttagtatt aagttttaat ttaaatgaag gtagtcttgg
     3061 ttgaaaaatg tctttttcta cagttattaa acatggatat tcaatctcac atacttgtaa
     3121 atcatttggc atatctattt caacaactat actttcatct ttaacttcaa ttaattttgt
     3181 aacgttagta acatgaggta tatctaaaaa ttcagctact tcaggtccca cctgtgctgt
     3241 atcaccatca gtagtttgtt ttccacaaat tattaggtct acctttccca tcttcttaat
     3301 accttgtgat atggtatagg aagtagccaa tacatctgct cctccaaatc tcctatcact
     3361 tattaatgct ccttcatcag ctcccatcat aaaactttct tcaattacct tctttgcctg
     3421 aggaggaccc atgctaagta cttttagatt cgctttctta tcttccttta ttctaagagc
     3481 tgtttctaaa gcatataggt catagggatt catttttgaa tctactccat ctctttttag
     3541 tactcctgta gtttcatcaa cttctactga agtcgttcca ggaacttgtt taatacatgt
     3601 caaaatttcc ataaagtaca ctcctttttt atttaactat aactgatact ccatcaggta
     3661 taactactac tttgctctcc ttaccaacta tactattcgc catttctaaa gcttctttaa
     3721 agttaaatgc atgtttcata tgcatttttt ttatcatttc aggattacac ttatctgtta
     3781 ccattatcac agtatattta gataatattc tcgctagtat ctgaaattcc cattggtctg
     3841 gaatcgtatc tactctatcg acacttctaa ttttatctaa aagttgtttt ggagaatctg
     3901 catttgctac attttcataa aaagattgac ctccatgacc atcattacat gctgaaatca
     3961 ttattattac tccaccgtct ttacaagtag cttcagctgc tgtcattcct ttaacagatt
     4021 gataaatatt ttggtctagt ggatatccac catttgtagt tacaacaata tcagatttta
     4081 tagaatttac ttttgataac tctgttacaa atctacatcc tttagtatgt gctttttcga
     4141 catcaccagc aaaggaagca attatttttt tatctttatc tattacaaca ttcaatatga
     4201 atgctaattt tgcaacttta gctgcatata ccatatcctc atgaatagga ttattattca
     4261 attttccagt tcttgaatta tcactatcta taaagtcact acagtgattg tacattatag
     4321 tctttgcaga tgcgattcct ggaagtacgc tttttctgcc acctgaaaaa cctgcaaaga
     4381 aatgaggttc tataaaacct tctgctatca aaagttctgc ctcatatgct agtttgttta
     4441 tatataaatc tccacctgaa ggcaatatcc ctactttaca catagattta tcatcagtag
     4501 atatatgcat aactatatct tcattatcta ctatttcttc tcccatttta taaattagct
     4561 cttcccttgt acttggccta tgaaatccag ttgccactat aatcttcaca tcaatttctg
     4621 gattaacatt cctaatcctt cttaatataa ttggcatagt tattctactt ggtacaggtc
     4681 ttgtatggtc acttgtaatt atgactacat tctttttccc cttaaccagt tcttccagag
     4741 aattagagcc tataggatta ttcatagatt cttcaactat ctcttcttga gtcttatgtt
     4801 ctatataatt atccgatttt gactccaaaa caccgagaaa acgttcatca ggtatttcta
     4861 aaatcattcc ttgttttgaa tacggtactt ttaactttac catttttaat ctcccattat
     4921 aataaactat aaaattggta tgtggtcata ccagttttac atttatataa tagtaccatt
     4981 atctaccatt tgcaagaata atttatttta taacaaactt ttaattttca ttttttctat
     5041 aatttttact tataaaatcc atatgttcct ttgctttctc acatgcaagt tctacatcat
     5101 tttctacaac tgcattgtat atagcttcat gctgtttaag caatatattt tcattaaaag
     5161 gttctgcgat tattttttct cttgcatctt ctataaatcc atcaaataat tttgatgctg
     5221 tcataaaaag actttgaatt aaataatttt tacttaatgt tgctagttta ctgtgaaatt
     5281 tttggtctaa taataccagt actcgattat tatctttttt attagtttct accctcattt
     5341 catctattat atgtttcaat tctataactt cagaagaagt tattctcttt gaagcaagtt
     5401 ttgcaatctc aatttctagg attattctaa gctctaatat atcttcaagt gtaccattat
     5461 taagtttaaa aatcatagat aaaggttcta ttaaagtttt ttctatattt gtacatataa
     5521 aatttccttc accctgtctg ctttctatca ctcccatagt ctctaatacc ctaatagctt
     5581 cccttatgga agttctactc acattcattt gttcagacag ttctctttct gatagtaatt
     5641 tatccccctt ttttaactct ccatttagta ttttatattg tatttgctca attacttgtt
     5701 cataaacttt tttactagct atactattaa acattttttc ctccaatatg ataatcgttg
     5761 ttgtaatata tatctattat atatcttaag ccattacatt catatatttt tattactttt
     5821 aatataatat cttataactc aaatttacag gtgatattat gaaaaaaaat aaaattattg
     5881 ctttttttgc tccatctatc atagttggag ttctagtttt agggattatc ctatacccaa
     5941 atgaagctat aaaatctgca agagaaggct tttctatatg gtctaatgtt ttagttccat
     6001 cactactacc atttataatt ggagcaaact taattgttga tttaaaaata gtggatatta
     6061 tagggtttat catcaatccc ataactagat ttgtatttaa tgtttcagga aagagcgctt
     6121 tagcttttgc tatatctaca gtatctggtt atccagtagg agtaagttta gcttctgaat
     6181 ttaggagtaa tggacaaatt tctaagtatg aagcacaaag acttgtgtct ttttgttcta
     6241 catcaggacc tttattcata ataggttcgg ttggtacagg tatgttccaa aattcaaact
     6301 taggttattt aatgattctc tgccattatc taggaactat attggttggt cttttcttta
     6361 gaaattatgg taatgaaaac cttccaaaga caaaattgga tttaaaaaca aatataaaaa
     6421 atgtaatcaa cattagaaat tcaagtggga atggtttttt tgttttattt ggaaatgctg
     6481 tatttaatgg agtaaacaca ttacttacag ttggtggatt tgtaattgtt ttttcagtag
     6541 tatttaaaat actatctctt tttaatgtaa tctcattaat agcatcagtt atatatttgc
     6601 ctctttcatt tttaggtgtt agtaaagaac tctgtcaagc atttgtaagt ggtctatttg
     6661 aaattactat aggatgcaat aaagtatcta gtataacaag ttctccagaa attttaagag
     6721 catctttagc tagttttttg ataggattta gtggattatc catcctagct caatgttgta
     6781 catttctatc aaaaacggac atagatttaa aattatacat actaagtaaa tttgcgcatg
     6841 gagttttagc tgctatcttt atatttatac tttatccaat tgcaaactct gctgtgttgg
     6901 tttctagctt tgctgataca tacaacgtta tatataataa tttaatctgg ttttattacc
     6961 ttagctatta tgatacaatt cttcaaattg tagtaataat ttatctaatc tcagccgtat
     7021 ttattgcaaa aaaacatatg aaaactttat ctacaaatgg gaaactaatg cgatataaaa
     7081 atactttttt cactaagaaa ttttcacgaa aaaaataaaa ctaaaaataa aactaataaa
     7141 acttaactaa atacttccat ttttttatta tatttgtcat aattttaata acctttttaa
     7201 ttagtttaac agcatagtta tttcatcaca atttaataat tttattagaa atatttttca
     7261 aaaaattctc cttaatcaca tatatcacac ttgtataagt ttactgggaa atttttccat
     7321 tatataatat ttcatttata ttatataata aataccgacc taaacttacc aaatcaaact
     7381 aagaggagtg gttgaaaatg gcaacaagac ctatagaaat agacgaatat aaaaagataa
     7441 tggaactatt acatactggc tttacttata gtgaaaatgg cgttgaaaaa agatttagaa
     7501 aaaatcctaa agttgcattg gcactaatgt tagaagctaa tttaggtcta agaatatcag
     7561 atattttaag attaaagata ggtaatttta aaggtaatat gttggaaata aatgaaaaaa
     7621 agacgggcaa attacagtat agaccagtta ataaaaatat aattgagtcc ataaatgaac
     7681 atgcaagaaa gtataatctt aaatcaaatg actacttagt aaatataaag acaaaggcta
     7741 ttcaaaaaca gttgaggata atttgtaaac acttaaatct gtataatata agtacacata
     7801 gctttagaaa gctctatgct actactcaat ttgaaaagag taacaataat ttagagttgg
     7861 ttaaagaact tttaaatcac agctctgtag ccactacaca gcgatatata agggtaactc
     7921 agcaagctat taatgaagct agtgaaaatt tctttattgg ttaattataa cgtatacatt
     7981 tttctattat cttattaatt caccagatat taaaaaagag tgtctcaaaa tgaacttttt
     8041 agttcataag acactctttt tttataaaat caaatatatt tttattattt caacactgga
     8101 aaaaaggctt atctctctat tttgagacac cctattaaat tttcataatt cttgcataaa
     8161 catattaatc tatcttcaat aattttttag ctgattcact gtaattattt ctttttacaa
     8221 tttccaaaac tctatcaatc tcatgttctg actttgcact tacaaaaatc gccaattctt
     8281 catcatctat aatatcataa ctatctacat atttatctaa acattctaaa attccatctt
     8341 tattattata cccaccaaat ctaactactg cactacactc atcttctata agtccactta
     8401 aatccaattt cccttttgaa aaagacttta tatttgaact tctattatct attatatcta
     8461 taatatctgc ataaacagca cttcctgttg cacctcttcc agctccctta cctacaaaag
     8521 aaagttcacc tactgaatca cctactaaaa taactacgtt gaactcatta tctatctttg
     8581 aaagcatatt attattgtcc actaatgctg gtctaacaaa tccacttact tctcctgatt
     8641 catacttact tctagcaacc aattttatct tacaatttaa tttttttgca tattctatat
     8701 ctttttcatc tattgaagtt attccttcta ataataaatc tttccaatat actatttttc
     8761 cataagataa agtagacaat atagataact ttctagcact atcatatcct aaaacatctg
     8821 catctggatt aacttctgca aatcccaact cttgtgcctg ttttaaagcg acatcatacg
     8881 gtaaattttc atcatacatc tttgataata taaaattaca agtcccattt aaaatagcgt
     8941 ttatgctctt tatattgttg ccttccagtg attctatcaa tggttttaaa actggaatac
     9001 cacctgccac agatgcttca aacttgatgc taacattatt taagttagct aattctataa
     9061 ggtcactacc tttttcagcc agtaaatctt tattagctgt cactacatgt atttttttat
     9121 ttaacgctct tgttacataa tcaaaagctg gatttaatcc tccaattact tcaactagta
     9181 tatcaatatc cttgtcaaat atatcttcta tatcagtagt tatcttatca aagtacttct
     9241 tatacttgtg tttttcttta tttttaacta aaattccaac aacttctata tctctttttg
     9301 aagtattatt ttctattaaa tcaattaatc cacttcctac tgttccatac cctaatattg
     9361 ctatcttcac tgtaacatac ccccatcatt ttgattatat taaattattt atatatataa
     9421 atataccaat aaaaaaagct tgagtataaa ctcaagccat atgacgggtt tatactcatc
     9481 tttcagcata aatgctgcag gatttagcac caatgtataa ttaaatatac tggttgccgg
     9541 gcttcacagg gccagtccct ctgccactct tgataagatt gctaatttaa atttatgttt
     9601 aatagtataa tacaatttct tccaattgtc actatttttt taacttattt ccctaaaagg
     9661 atataaacta ttatctaaca gctcttttag ttggtttacc cttttttgta acattattat
     9721 ttttagtctt tgctttcgcc tgttcatgtc ctggaaaaag tattttaggc tcttcaatat
     9781 tcctcttgta aatagtaaat ttaaaaccaa tagcctgcac aaattcagct cttaaagctt
     9841 cacatactgc attagcagtt tctttagtat caagacctgc attttctaat atagtaactt
     9901 ttactaattc tctagttctt aacataccat ctaattgctc taagaaactc tctgtaacac
     9961 cttctttacc aatttgtgta gtaggcttta aagtatttgc caaagatctt aaatacgccc
    10021 tttgttttcc ttttaacata ttctcacctc tataattcta tacttaaatt atctataatc
    10081 tatatttaaa ttaattgtaa aactcaaatt ctaattcata aattttaacc acatctccat
    10141 cttctattcc catttctcta agcctatcaa aaacaccttg actctccata gctttttgga
    10201 aatattgaag ggattccata tcatcaaaat ttacagaata cattattctt ctaagtgctt
    10261 tacctgtaac tacatacaca ccatcttcaa tatctatact taactcttct tcagtaccaa
    10321 tatcaagttc tggtctatac atttcttctt ctgaaactag ctccacatct tctacttctt
    10381 ttagcatttt agaaacatag gctattactt catctatgcc ttgacgagtt gctgctgaca
    10441 ttttaaatac tgtatatcct ctgccttcta attcactctt aaacttctca aacttactct
    10501 catcttctaa tatatcaaat ttatttgcaa ctacaacttg tggtctcttt gaaagctttt
    10561 cattgtacaa tttaagctca tcatttatct tgtcaaaatc ttcaataggg tctcttccct
    10621 ctaatccaga tatatctaca atatgtatca atacctttgt tctctcaaca tgtcttaaga
    10681 actcatgacc taatcctatg ccttcagaag caccctctat aattccaggt atatctgcaa
    10741 gtacaaaact atctccaaac ttagtttgaa caacacctag atttggtgta agtgttgtaa
    10801 aatgatagtt tgcaattttg ggtttagctt tagttacaac tgatagaaat gttgatttac
    10861 ctacatttgg aaatcctaaa agaccaacat ctgctatcat tttaagttca agagttatcc
    10921 atctttcttc tccatctgta ccagattttg caaaagctgg agcttgtctt actgcatttg
    10981 caaagtgttg gttaccttta ccacctatac caccttttgc aactatagct gtatctcctt
    11041 ctttttttaa atctgctaat acaagacctg tagcctcatc tcttattact gtacctgctg
    11101 gtacttttaa tactaaattt tctccatttt taccagctct ttttcctttg cttccgtcgc
    11161 caccattttg agcttgatat tttttctggt acttaaagtc cattagagtt ctaagcccca
    11221 aatcaacttc aaatatgatg cttgctccac gaccaccatc tccaccgtct ggaccaccag
    11281 ctggaacgta tttttctctt ctaaatgcta ccgaaccatt tccaccattt ccagccttaa
    11341 caaatattct tgccttatct ataaacaaat tcttcaccta cttaattaaa aacttttaat
    11401 tagcattacc aaaaatacca ttataaattc atcagtatta taagaaaaag gagtgcctca
    11461 acactccttt tatgtatatt actcagctat agctactgga tatatactaa ctttttttct
    11521 cttcttgtct tttctttcga atttaacagt accatcaact aatgcaaata aagtatcatc
    11581 actacctttt ccaacattag ttcctggatg tattttagtt cctctttgtc taactattat
    11641 acttccagca gttactaatt gaccatcgaa tctcttaaca cctaatcttt tagatataga
    11701 atctctacca tttttagaag aacctacccc ttttttagat gctaataatt gtaaattcat
    11761 gtttaacata tggagtcacc ccctactttt ttaaaatttt tatatttttt gggtattccc
    11821 gtttaatttc tttaaccgct aattgaaagt gatttagtaa taattgtgcg tcttttacat
    11881 gtgtatcttt gactatacac tctatatgac cttcttctcc tactactttt tcaaattcaa
    11941 tcttttgtaa tttgtctaaa gaatttataa ctgcaatagc attagatgta actgcagcac
    12001 atactatatc atagcctatt tcagcaaaat cagcatgtcc ctttaaacaa aaaccttgca
    12061 acaagaaatc atcattataa taatatttaa cttttatcat aacttaacca ataactaagc
    12121 gtttattttt tctacaacta tcttagtgta tgattgacga tgtccttgtt ttcttctgta
    12181 atcttttttt gctttatact tgaaaactat aactttctta gctttacctt gttcaacaac
    12241 tttagcttga acagatgctc cttctactac tggagaacct aattttaatt ctccatcttt
    12301 agagcaagct aatacttcgt ttaaagtaac aacatcacct gcgttagctt ctaatttttc
    12361 aacgaataat acatcacctt cagaaacttt atattgcttt ccaccagtct ttactatagc
    12421 gtacattaaa tacacctcct cggcctcaaa ctcgccagta caaggtactt catttgaaga
    12481 tttaaacctt tactgtgcgg cattacaatt attaattata aaatatttac attttattgt
    12541 caactatttt atcgaaaata acattggttt tattgtgctt taatgtacta tttgcgttta
    12601 aagaaatctt tatattatat ttttcaccaa ttttatcaat tatatccata taatattctt
    12661 ttatttgttc aaaaataaaa tcattaaatt ctaccattat gtttttgtaa acagtatgtt
    12721 cacttattct cataatttct ttttcaatac tatctagtat ataatgtaca gattttattg
    12781 tttgttcacc atcacaacat ggacaagaca ttaaataata cttatcaatg gattcttttt
    12841 ctctacgcct agcaacttct actaaaccaa gttttgtcat acctaatact tcagcttttc
    12901 ttttatcttt attaaattct tcttctagga tgtgtaaaat ctccttttga tgcttctttt
    12961 tatgcatatc aataaaatcg attattatta tcccagctat gtctctaatt ctaagctgtc
    13021 ttacaatttc ttttgcagct tcaagattag ttttataaac ggtttcatca agtttaccag
    13081 ttccaataaa cttacccgta tttacatcta taaccgtaag agcttctgtt ttttcaatta
    13141 ttaaataacc tccactcttt aaccatactt ttttatttaa taatttttct attttagatt
    13201 ctattctgta taaatcaaat acatctctat tttcctcaag acacagttta tcaacataag
    13261 tcttatctat acctcttaaa atagacttaa gttcagagta cttatcataa ctattggtaa
    13321 ttatagattc aatatcatca ttcacattat cttttacata tttagttgca aaatctaaag
    13381 acttatataa taatttcgga cctataccaa gtttatattc ttttaaaatg ttttcatact
    13441 ttaatttcaa ctcttctata tctttcttta tttcatcatg agtgcaacct tgagcctcag
    13501 tccttattat taatcctaga ttttccttat ttactgcctt agtaattttt tttaatctaa
    13561 atcgttcttt ttcatcagta attttatttg atattgtagt tctatcattg gaaggtatat
    13621 agacaatata ccttccagaa atactaatct cagtgtttaa ttttgcgcct ttagttccta
    13681 tttcttcttt atttatctga accaaaatat cctgaccaga ttttatagta cttccctttt
    13741 ttaattgtaa ataagcaagt ttcttaaatc caatatctac aaaacaagct tctattcctg
    13801 gtataacttt ttttacgatt ccacgatata tattggatac agtcttctta tttaaattgt
    13861 cttcaacaaa aagttctgtt aatctttcat cttcaagtac tgctgttttc tgggacccta
    13921 ttagggactc aataactatc tttttcacaa cttcacctct cgagcttaca taggagtcac
    13981 taattcccca tcttctaata catataaatc tcttctcaat atatcaacat ctaatgggtc
    14041 aatatcaagt tcaaacatct ctaatatctt tggtataagt atattagtat ttaaattagt
    14101 ttttgagcct gtagctatag tagcagtaag agtaatatgt tcatcttcta aatttaaaac
    14161 atcaaaggtt cttatcatag gtcttatatc tacttcaacc atcttacctt ttttattctt
    14221 ttttgttatg attatttctt tattattcat aaagtcaatt acttttcttt ttacaaactc
    14281 tttcgtcata ggtcttttca aatctatatt aaacaaatat tctccatagt ctatcacaga
    14341 agaaagagaa ggtgtttgtc tatcaatttc ttttgctttg ataaaatcta tcccatctgg
    14401 aagttgaatt gatactttat ttaaaaattc ctctactgat aaatcatctt cttctatttc
    14461 aatatctaca tactcacctt gactttctgt ccctaaagcc aaagcattgc cataactcat
    14521 tttagggtga ggattaaatc cttgagaatg tgataagttt atttcagctc ttctaaacgc
    14581 tctttgtaac aatctctgaa ggtcaagatg tgatatatat atcatgtcgc cttccttttt
    14641 aaattttact cttattatct tactcatatt agcaaagccc ccttcctata tcatgtatat
    14701 ttattccaca accattgcag ttatgtctac aatcagaagt gattttttct tttttagcat
    14761 tttcgtcttc tctaattaag aattttttac taatacctac atctatatgg tcccaaggaa
    14821 atacctcttc atagtttcta tttctatgtg cataaaaatc tatagaaaga ttattctttt
    14881 ccatagcttc tttccatata tctaaattaa aatgttctgc ccatccatca aatttagcac
    14941 ctagtctaaa tgcatcatat ataacttttc ctattcttct atcgcctcta gctactactg
    15001 cttccatcaa actagtcttt gaatcatgat aattgtattt tatattgtta tttcttaatt
    15061 tatttactaa atgtctctgc ttattaataa cttcttctgt agtatcttga ccatgccatt
    15121 ggaaaggtgt aaatggcttt ggtacaaatg tagaagtact cactgtaaca ctaaaacttc
    15181 tctttagctt tcctccattt acttttctat aaatatcaat aactttataa gctaatttag
    15241 ctataccatc taaatcttca taagtctcag ttggtaaacc aatcataaaa tacaacttaa
    15301 cactattcca ccccatctca aaagctcttt ctgttgcatt ttccaaatct tcttctgtaa
    15361 cacctttatt tataacatct ctaagtcttt gagttcctgc ttctggagca aaagtaagtc
    15421 cagattttct tacttgttgt attttatcag caatttccat tgaaaaatta tctaatctaa
    15481 gggatggaag cgaaatccca atattattag atgcatattc atctaccaaa taatcagtca
    15541 actctgataa cttactataa tcactcgtac taagtgatga taatgatatt tcatcatatc
    15601 cagtattctt aactaattta tctaatattt ctttaagtct tttaacactc ttttctctta
    15661 ttggtctgta tatcatacct gcttgacaga atctacatcc tctagtacat cctctaaata
    15721 attctagtac tattctatca tgaacagtat ctatataagg tactattagt ttttctggat
    15781 aatctacagt ctcaacatct tttattatcc ttttagtagg atttttagta atcccttctc
    15841 tattaggagt tacactttga actgtaccat cttcattgta cttgacatca taaaaacttg
    15901 gtatgtatac tccttctatg ctagatattt tatataaaaa atcttctcta gtcgttttat
    15961 ttttcttcca ttctttatac tcatttacta cttctaaatt aacctcttca ccttcaccta
    16021 atattactac atctacaaaa tctgctaatg gttcggaatt ataagcacaa ggaccaccaa
    16081 ccattataaa tgggtcttcc aaagttcttt cttcttttaa tattggtata ttggctaaat
    16141 ctaatatatt taagatatta gtgtatgaaa gttcatattg aagtgtaaaa gtgacaaaat
    16201 caaagtttgt tataggctct cttgactcta atgcaaataa tggtatactt ttttctctca
    16261 ttatattttc catatcaact gcaggagaat acactctctc acaaaataca tcttcatcct
    16321 tatttattac atcatataat atatgactcc ctaagtgact cataccaact tcatataaat
    16381 ctggaaagca atgtgcatat cttatcaatt cactattact agtatcttta tgaattgaat
    16441 ttatttcatt tccaagatat ctagcaggct tttctacttt ttttaagatt tttttcaagt
    16501 ctactttatt catttattaa aattcctcca aacaagctct ttatttttat tcttatttat
    16561 aagtaatatt atacacgata aaatattttc tatcaaaaat ataactaaaa tattgaattt
    16621 aaaatacata ttattcacaa aatagaatag ctaaaaatat ttaagaatat tttctattaa
    16681 atttttattg aaatacaatt tttttacaaa gtatgatata ttataaacat aaaactattg
    16741 ttttacatga cttatcatga catcttttgt ttcattcacc tcaaaatggg gtatgttact
    16801 tatataatga taaattttag gagggttaaa aatgagtaaa gtttttgaag taaaaaaaga
    16861 tatctacttt actggagtag tagatgaagg tttaaaagta ttcgacataa taatggaaac
    16921 tgagtttggg acaacttaca actcttattt aatcaaggac gaaaaaacgg ttttatttga
    16981 cactgtaaag gcaaatttta aagatgagtt tttaagtaat ttatcagagg ttactgatat
    17041 agctaaaatt gactatgttg taattcatca cacagaacca gaccatgcag gtagtttaaa
    17101 atacttatta gatataaatc ctaatataga agtttattgt actaaagctg ctaaactata
    17161 cttagatggt caaataaata gaccttttaa ctgtcatgta ataaaagatg gtgaaatttt
    17221 aaatatcggt aaaagaaatc ttagatttat aactgctcca ttcttacatt gggttgatac
    17281 aatgtttact tacattgagg aggacaagac tcttttaact tgtgatgcat ttggttgtca
    17341 ctttgcaagt gttgatgcag aggtagtgaa tagtgaagat tatctaaaat cagctaaaca
    17401 ctactatgat tgtatagtta agccatttgc taaacatgtt ttaagtgctg tagataaggt
    17461 ggtaggacta aacatagaat ttgatactat attgacttct catggtccaa tgcttactaa
    17521 agacccaatg gctgctgtta aaagatatgt tgagtggtct acagaagctg ttaatacaac
    17581 taatcaaaat caagtttcta tattctactt atcagcttat agtaatactt tagagatggc
    17641 taaaaagata aaagaaggac ttgataaaga aggtgcaaag gctgaattat atgatttaga
    17701 agatatgaca ttaacagaaa tgcacgatac attagtagtg tcaaaagtaa tattactagg
    17761 ttctccaact ataaacaaaa ctatggttaa acctatgtgg gatttattct ctgttataga
    17821 cccaatggct aaccaaggca agatagcagg agtatttggt tcatttggtt ggagtggtga
    17881 aggtataact atggctgaga ctttacttaa atctatgtca ttcaaaatgc ctgtagaatc
    17941 tcttaagaaa aaattcttcc ctagtgaaga gacattaaaa gagtgtatgg catttggtgc
    18001 agaatttgca aaattagtta aatagccatt aatcaaacat aatattattt aaaataaatg
    18061 attttatatt taaaaataaa ttaaaatgga gttgtctcaa aattaatttt tctaatctaa
    18121 ttttaagttc aactcctttt ttattttcta aaatgactga tataatgata aatgcaccct
    18181 ttgtgcgagg gtgcattatt tataatatgt tctatttgta cactagtata tttgtattaa
    18241 ttcacatatc tagcgtatgc taccatccct cttccagaat tagatgccgt ttcaccaatt
    18301 tccactttaa taattacatt attttcttta cacttttttg ataattctgc tgcagaaagg
    18361 ttcttaccag taaaatctaa agaaaatggc ttatcatatt caaaaggata ctcatcaggt
    18421 acaccatctg gagctaatgc aaccatacca tatggtgtat tcataaaccc tattacagta
    18481 actgtatgct ttgatgggtc taaatttgaa tttttgcttg taaacttaat tgtatttatt
    18541 gatgagttga ttaaactaca cggaactccc tcatacattt caatttttgg acgtagatta
    18601 tttccataaa cttcatcaag tattgcccct tgttgatctg ctatcatagt gtgattccca
    18661 ggtccaactg gcgcagaagg cttagctggc gtaggtttct taacagttgg cgttggattc
    18721 tgtgtcttta ctacttcgcc aacagctgga tatgctttct tgtcaaatgg taatgggtga
    18781 gtaagactct tgtatatgat gtctgctcct gctgatttgt tggcatcaat atctacatga
    18841 tacaaacgtc catcctcaaa acgaataaga ttccactgat ggtcatttgt tgtactatgt
    18901 acataataac attcaattcc catagcatca cataaagctt tcaagctcct acacattcca
    18961 gaacaagcac cttttttagt aaccaatgta taagcagtat tggctccctc tgctgcgttc
    19021 cagttaacat atgttgtgtt gtttttaatg tgattataag caaccctaat cttagcatct
    19081 gtggtcatgt tctttgtaat tttcttgttc ataaagtctg ctgttgcttg tgctaccgaa
    19141 ttaagttgag cttgtgataa ctttggacca tatatactgt tggttccagc tacataactt
    19201 ttcggtgtct ttgcagtaac atctttagct gtcataccat aaactgttac actcatacat
    19261 gaggttaaaa ccatcattgc aatcaatagt ttagtagcta atttcctgat ttttttcata
    19321 ttagcattct cctttataat atattttact ttcaattccc taccattaat taagttcatt
    19381 ttagatttaa aatatggatt atcaacatgt atgcatagca attcacgtgg tcatagaata
    19441 tttagatttc ttggaaaatc tgttctatat ttcctatata gttcgtatac aatccaactc
    19501 acaaacctgc atttttgata tcatcacaat aaaatgataa taattaatat attcaaaaat
    19561 gttggtctgt aacaggtatg tcaattttcc aattattggt aatggcgaga gtgaggagaa
    19621 ttgtcaataa tacatttaaa attaaaattg tatacacttc atacattctc ccctaacaac
    19681 attatacaga ttataatttg tatattcaat acattttttg gaaagtataa ttatatcaaa
    19741 atatgtgatt atgtatagat acaatccact atggagaaat ataaaagaaa agagtataac
    19801 aaacaataaa aaataagatg attcaaattt tattttgaaa catcttattt actctacaaa
    19861 ttaagattat ttggtgaatt atttaattat tattttttat taaagaaatt ttaaatctta
    19921 atataaaata cgctattata cataataatc caaatgcaaa tgctactgga attaacatct
    19981 ccgattcaaa ataaaagaaa tattctaaga aaatatctgg atttactata ttcccattct
    20041 tgtctaaaaa atcttttcca ttatatccta aatgctgtat aaaattactt atgttattat
    20101 aatctgcttc cctctcatat accatattag cattcaatcc agaagaactt aaattagtgt
    20161 ttccatcttt catatctatt acatatagtc taagagcttt gactttacta tacttatctt
    20221 tatcaagtat tttttcaagt tctaacttat attcaccaat ctttgtcata tcatgtcttt
    20281 ctgccaattg ctttatttct ccatcaagtt cttttcctaa attatacata ttatgttgta
    20341 aactattatt gtaataccac ataaatccag aaattaaaaa tgcaattata cttatgtaac
    20401 ctaatacttt tttaaacccc attacaattg atttatgcct atccaatgat aaagatagtt
    20461 cttttattat attacataat tcatactgca tttcatcacc atcatcaaat ctttcaattg
    20521 cttttttaca tgcttcttct tcatctaatc cttctaattt aaattcattt gcagattcta
    20581 taagatggca tctcatttcc tgttttaatt ctatagtact tttattgtca cattttttat
    20641 atagtttatc taaatattca tctattagtt tcataaatta atccacctct cccaaaaatt
    20701 gattcataac ctttttaata aatctccatt cttgtttctt tatttctaaa aaatctttac
    20761 cttcattagt taaattataa tattttcttc ttccacctga actttgttca ttgctataat
    20821 aagatttaat taaattttta gattccattc ttttaagtgc aagatacatt gttccttctt
    20881 ttaattcaaa ctcacttctt tctcttactt gctttgcaat ttcatatcca taacaatctt
    20941 tcttttctaa tatagaaaca attaatatat cgatataacc ttttaacact tctttattta
    21001 actgcattat gtaccaccgc cttttcacct actactctat aatgtatagt acttatattt
    21061 actataatat tacatagtac tctatattgc aaggtttata ttctacaata gacttaaaaa
    21121 taataaatag ctgtatatta atatttaatc tataaatact aatatacagc tatttatcaa
    21181 attttaaaaa taaatttttg ttttattaat gttgtcataa caacatttac ttg
//
LOCUS       DBGJFU010000067        21100 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.067, whole genome shotgun sequence.
ACCESSION   DBGJFU010000067 DBGJFU010000000
VERSION     DBGJFU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.067"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            163..1173
                     /locus_tag="ACOIAS_002261"
     CDS             163..1173
                     /locus_tag="ACOIAS_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HGZ2701969.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1284..1772
                     /locus_tag="ACOIAS_002262"
     CDS             1284..1772
                     /locus_tag="ACOIAS_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HGZ2701970.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1948..3876
                     /locus_tag="ACOIAS_002263"
     CDS             1948..3876
                     /locus_tag="ACOIAS_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2701971.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4053..4463
                     /locus_tag="ACOIAS_002264"
     CDS             4053..4463
                     /locus_tag="ACOIAS_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HGZ2701972.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4485..4787
                     /locus_tag="ACOIAS_002265"
     CDS             4485..4787
                     /locus_tag="ACOIAS_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HGZ2701973.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5004..5696)
                     /locus_tag="ACOIAS_002266"
     CDS             complement(5004..5696)
                     /locus_tag="ACOIAS_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGZ2701974.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5722..6909)
                     /locus_tag="ACOIAS_002267"
     CDS             complement(5722..6909)
                     /locus_tag="ACOIAS_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2701975.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7245..9122
                     /locus_tag="ACOIAS_002268"
     CDS             7245..9122
                     /locus_tag="ACOIAS_002268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2701976.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKVGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9432..10838
                     /locus_tag="ACOIAS_002269"
     CDS             9432..10838
                     /locus_tag="ACOIAS_002269"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGZ2701977.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10866..11714
                     /locus_tag="ACOIAS_002270"
     CDS             10866..11714
                     /locus_tag="ACOIAS_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2701978.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNEKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12216..12908
                     /locus_tag="ACOIAS_002271"
     CDS             12216..12908
                     /locus_tag="ACOIAS_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2701979.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            12905..13840
                     /locus_tag="ACOIAS_002272"
     CDS             12905..13840
                     /locus_tag="ACOIAS_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2701980.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            13988..14911
                     /locus_tag="ACOIAS_002273"
     CDS             13988..14911
                     /locus_tag="ACOIAS_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2701981.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            14895..15665
                     /locus_tag="ACOIAS_002274"
     CDS             14895..15665
                     /locus_tag="ACOIAS_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701982.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15675..16457
                     /locus_tag="ACOIAS_002275"
     CDS             15675..16457
                     /locus_tag="ACOIAS_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2701983.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            16834..17370
                     /gene="rbr"
                     /locus_tag="ACOIAS_002276"
     CDS             16834..17370
                     /gene="rbr"
                     /locus_tag="ACOIAS_002276"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HGZ2701984.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17402..17791
                     /locus_tag="ACOIAS_002277"
     CDS             17402..17791
                     /locus_tag="ACOIAS_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HGZ2701985.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            17828..18214
                     /locus_tag="ACOIAS_002278"
     CDS             17828..18214
                     /locus_tag="ACOIAS_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HGZ2701986.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18341..19783
                     /locus_tag="ACOIAS_002279"
     CDS             18341..19783
                     /locus_tag="ACOIAS_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HGZ2701987.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20080..20895
                     /locus_tag="ACOIAS_002280"
     CDS             20080..20895
                     /locus_tag="ACOIAS_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2701988.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         7794 a         2462 c         3877 g         6967 t
ORIGIN      
        1 tgggtattgt atactggaat tagagtaatt aatttatttt ttaacataca aatgatttta
       61 tgaaaaaaaa caagactcaa aaatttttat attctttgat agaataaaat tgagagtaaa
      121 tttattgaat tttataaaat ttataagggg tgagtctaag atatgcatga agataaaaat
      181 gatatatttg aatcaataga tactgaggat tttgataata aaacagaaga agaatgttta
      241 gagctagtaa gaaaaaatgg attgaattta atatacataa aaaatcaaac agaagagata
      301 tgtctagagg ctgttagaca aaattgtaat gcaataaaat gtgttcagaa tcagacagaa
      361 aaaatatgta tagaagcagt taaacaagat tggagaatgt tagaatttgt agaagagcca
      421 acagaagaaa tttgcatgtt ggctattaac caagatggaa cagcattaaa atatatagaa
      481 aatcagacag aggagttatg cttaagagcc gttgaaaaaa atggtgcagc tttggagtat
      541 gtaaaagaac aaacagaaga aatatgtata gaagcagtta gaaacagcga atttggattg
      601 gcaagagtga aaaatcaaac agaaaaaata tgtatggaag cagttaagca ttgtagctat
      661 aatctaaaat gggtagaaaa tcaaacagaa gaaatatgta tggaagcagt gagacaaaat
      721 gggcttgatt tgaaatttgt aaaaaatcaa acagaaacaa tctgtttaag agcagtaaga
      781 caaaatggaa tggcattaga atttgtaaag gaacagactg ttggtatatg cttaaaggca
      841 gtaagacaat atggaatggc attaaaattt gtaaaagaac aaacagaaga aatttgtaca
      901 gaagctataa aacaagataa aagagcactt agttttgtaa aaggagataa ggaaaaatac
      961 aaggctctat atgataataa tgaaccattt gcaaagagat atgttagaaa tgtaatagaa
     1021 aaagaaaaca gagcattgat taaaaaagga gaagaaaatg caaaaatcaa aattgcagga
     1081 aaactttatg atagaggcat gtcttttgaa gatatatcag atatagttga gattgaaata
     1141 agcaagttaa aagtgagttt aggtgttttt taagggaaat agttaataaa aaaataatcg
     1201 actagctctt tgtaggacag caaaattagt gatataattt gatgtataat aaattatttt
     1261 tattagataa gggggaatta aagttgaaga ttacagtatt ataccaaaca agaagtggaa
     1321 acacagaaag ggtcgctaag ctgatagaag agggcgctaa aaaggtggat ggagtagatg
     1381 taaagcttat gagacttgat aatattgatt tagattattt aaatgaaagt aaagcagtaa
     1441 tatttggaac acctacttat ttagcaaata caacttggga agtaaaaaaa tggtttgatg
     1501 aagattcaaa aaaagttaat ttagctggaa aattaggagc tgtttttgct acatgtgatt
     1561 atatatgtgg aggtccagat gttgcgatac ttactatagt aggacatttg atggttaaag
     1621 ggatgcttgt gtattctgga ggtggttctt taggaaagcc atttatacat ttaggacatg
     1681 tacattcaat tgaaggtcca gagttacaag atgagaaggc tataatattt ggtgaaagaa
     1741 ttgctactaa ggcaaaagaa ctatttgctt aatttctata tgcataataa tgattagagt
     1801 aaatttaggg ttttaatgga gatttgaagt aaaagttata aaataaaaaa ttttccataa
     1861 aattactcaa gatgaattat aatataatta taaataaatt atattattaa aatatttaaa
     1921 atcaaacgtc aatagcgagg gaagtttatg gtatggcata tagaatatga gattttttct
     1981 gcaattatag ttattttttt aatggtatac ttttttagaa gtaaatttat acctacttta
     2041 caaaacaaaa tatattgtgc acttttaata ttctcatttt tatttataat ttcaaatatt
     2101 ttaggttcat tttgtctaaa taatatagac aaaattccaa tttttattac ttttttattg
     2161 aatcagatat acttattgtt attgccaata ccagcagcac ttgtatcatt ttatgttatg
     2221 gctattattt atcaagatat aaggtatatg aaaaaaagat tattattttt atctatacca
     2281 cttataattt gtataatctt atctattaca aatcctttta cgcatattct atttggattg
     2341 tcaaaagaaa caggttatat acgaggttgt ggatatgttg caacttttgc atcttttttc
     2401 ttttatgcaa tatattcagc atttttatct tttaaatata gaaaagcaat gcatgaatca
     2461 aaaatatggg ctattagagg ttttcttata gtttcaattg tggcaattat cttacaggca
     2521 atttttttac agtatcttct tacaggaact gcatgtgcat gtagtttact tttgacatat
     2581 ctttctatgc agaatcgagg tcttattatt gatgacctta ctggtgtact caatagacaa
     2641 tcttttattc aagagctaga tttgaatatc aatactgaac aatcaggatt tattattaca
     2701 attgcgcttg atgattttaa gtttatgaat gaaacttttg gaacaaaaaa tggagatatt
     2761 gcactaaaag aagttggaaa atatttgatt cagatacatg atgtaaacca tgtatataga
     2821 atagggggag atatattttc tattgttgtc aatagtcaat taggtataga accagatgat
     2881 attatcagaa agatagaaga aagatttaaa aagccttggc tagtagaaga gataagtttt
     2941 aatttagcaa ctagtattgc tgttgtttat tacccagaaa atgcagaaac gacagaagat
     3001 gtaattgttg ctatagattt ctctatacat gaagcaaaac gttctgagtc aaggcgagtt
     3061 gtttatgcag atgcgtctat aagtgaaaag ataaagaaga aacatgttat taaaaattgt
     3121 ttaaaagaag cattaaaaaa tgatgggttt gaagtgtact accaacctat attttctaat
     3181 agcaggggca ggtttacttc agcagaggca ttacttaggc tagaacacaa agagttaggt
     3241 tttatacctc ctgatgaatt tattccaatt gcagaacaaa caggattgat taactcaatt
     3301 ggattagtag tctttgagaa ggtatgtaga tttatagcta gtgatgagtt tgaagattta
     3361 aaattggata atatagctgt gaatctttct gtagtacaat gtatgcaaaa aaacttagct
     3421 gatgatttac tttatcttat gaataaatat aatgtatcac caactaaatt taaacttgaa
     3481 attactgaga ctgtggctat gggctctttt aatataatta aagaaacaat ggaaagatta
     3541 attgatttag gtgttaagtt tgcacttgat gattttggaa ttgcttattc tggagttact
     3601 aatatgcttt ctttaccatt ttcagtaatt aagttagata agagtttgat ttggtctatg
     3661 aatgaagata gtaggcataa acttacagtt gagacgatta tagctcttat aaataaattg
     3721 aatatgaagg ctgtagcaga aggtgttgaa accattaagt gtgctgaaga tttaatagct
     3781 atggagtgtg aatatttgca agggtattat ttttctaaac cagttccaga agatgttttt
     3841 aaaaatttgt tgaaagaaaa tacttttaca agttaacacc ctttattttt ttaattacaa
     3901 ctttgttcat tgacactgcc agcgatatat tataaaatta acacaataat aaatattaaa
     3961 tttattatac atattattaa tattaattaa tggagtaaag atttattata tattttattt
     4021 aattatccta aagtgagaaa ggaagatgtg acatgaataa aaagaaaata ttagtgccaa
     4081 ttgatgggac agaaagaagt atgcattctt tggaatttat aaaaggaata tttaaaaaag
     4141 atgaagttga agtagaaata atgaatgtaa aagaactagt atttattgat ggcatatccc
     4201 tggctgaaga gataaaaaat tctgaaaatt taggaagaag aatcttggat aaagcagcag
     4261 agataatggg agagtatgat gttaaagtac attttacttt tggatatcca ggagatgaga
     4321 taattagaaa agcaaaagaa gataatgtag attttatagt tatgacaaaa tctactaaaa
     4381 aaggtcttac aagaatgata ggttcagtaa cagcaagtgt agttaaacaa gcaaaatgta
     4441 tagtgatgat tgtaccggag tagaattaga aaggtatttg aaatatgaat aaaaagatag
     4501 tagcagtttg tgaaagtgaa agaagtttaa attctcttaa gacagctgca aatgatttag
     4561 gatttgattt agtttatgaa attcaagaaa atgataatat aaaaaatgaa ctttctattt
     4621 atgatataga agatgcatcc atagtgttat ttgttacaag tgattctata gaaagcatag
     4681 agaagattga tagatttata gatagagaat tttatgaagt tgatccaaag tatataatag
     4741 aggatgcaaa atctatagta agtgaaattt taatagactt aaattaatcg tgggtgtcgc
     4801 actaagaata gagcgacgcc tttttatata ttttacacaa gaaataaaga ggttgtatca
     4861 aaatatagat aagagtcttt ttcattgttg aaatgatgaa aatatatttg atttcataca
     4921 aaaaagagta cctcataact aaaaagttca ctttgagaca ctccattttt atatgttagt
     4981 tgcatttaag gtaaaataga agtttaatca tgttgaaata actgaattac atcttctaaa
     5041 tgattgtaca ttgatgtata acctaagtaa gagtctttac tatcaaatgc aatcattatc
     5101 ttgttatggt ggctaatcat atccttgaga gaatttctat atttaaaacc aaagtattgg
     5161 ttagaagaaa ctgttttagg tattaactca taatatttta taaaaattgg attatgagaa
     5221 gctaaaatta tctctttgtg aaattgcaag tcagcatcat aacattcttc tatcaaaccg
     5281 tgagaaacct tatccaattc ttctattgac tttaggtaag tatattttat tttttgaagt
     5341 tctttagatg tagcatttaa aattgctagt ttagtagctt cactttcgat aagttgtctt
     5401 gctcttaaag attctacata aaattcttgt atgttatgat ttttatagat ttcaccatca
     5461 tcaaataggt caactttttt aaatccttca actataggac ttgaataagg cacagctcta
     5521 agtattcctt ccttacagag tatatttatg gcatttctta caggtattct acttatgtta
     5581 aataggtcag caatctttct ctcacttggt aatttactat taattttaaa agttccattt
     5641 agaatgttta atttaatata atcaataatt ttttcagtgg catttaagct tttcataaaa
     5701 actcctaaac atataatttt tttatgattg tattatatca tagttaagag ttaaatctac
     5761 ttttttataa tatgtataag acaggataga ggccaaaata gctataatag ccaagataaa
     5821 gtatccatat tgtattgaag tatattccga tattttacca actatcattg gaaatacaga
     5881 cattcctata gcataaattg cttgaaaaaa acccattgca gtagatttct gatgttttgg
     5941 tacatttttc atagactcgc tagtaaggta agaaaggagt atacctgtag atatacctgg
     6001 tagtacttgt agtacaaata ttatattaat attttttatt gttggaacta aaaagcaata
     6061 ggctgaaact aaaataaaaa ctaaaggaat ccaaaatttt ggcccatact tagtacaaaa
     6121 tttagtagaa gcaaagccag aagaagcaac agcacacacc atgtaaaata tagaagatag
     6181 acctatgagg gtagaactag aacctaagtt tttaagaatt tgagtagtaa atgacattgt
     6241 tgtagacatt tgaataccct gttgaattaa tgccataaag gaaaaaaata caagtctttt
     6301 atccttgcag atagataaat attttttaat tgatattttt tcaggtattt tttttgaatt
     6361 atgatttttt atatttaagg ctagaatcgt tccaataact gaagacaata cactcatgat
     6421 acataaggct gtcattccta atttttcata aaataaagta ctcataacaa atccaatcaa
     6481 cattcctaga ttgtttacta atattattgt acttgtagct ttttgttgat gttctgtaga
     6541 aaaaaagttg gtatagtgaa ccatgtaaga aatccaagtt gatgatgata ttccagataa
     6601 aatgttagct ataagaaaac cattgcctcc aggagaaaag actcttataa gagatgctaa
     6661 accagcaaag aaaattccag acagtataaa tattttatgt ttatttatgg aatcagcaaa
     6721 tactccaaca ggaagcctta ataaacattg tgagattcca tatgcaccta ttattatacc
     6781 aatcatattt gtagatacat tacatgcact taaatatgga ttttggaaag gtatatatac
     6841 atattgtgaa aaccagaaaa atgtaacaat agaaagtaaa agtaattttt gattagatat
     6901 ttttttcata ttatcctccg tatggtattt ttataatgca aataatatac catttggatt
     6961 ttttaatcaa gtactttctc tattaaaaaa aatttaaata tataattatt atttacaaaa
     7021 aaattatact ttatagttga attaagaaaa cgcttatgat actataaagt taacaaattt
     7081 gaatatataa cagtagacgt gttactggta atgcaggcaa gatcgaatca atttttatag
     7141 attatctata gagtactatg ggtataatct atttatttgg ttcgattttt ttgtttaatt
     7201 ggccataaaa caaaaagcct atagttgaaa atggaggatt ttttatggat tataaaaata
     7261 tagctcaaga aattctacta aatgtaggag gaaaagagaa tgtaaatgaa gtgactcatt
     7321 gtatgacaag acttagattt aaagtcaaaa gcgcttctaa agtgaataag gataaattat
     7381 ctaaaactga aggagtaata actgttgttg agagtatggg tcaaatacaa gttgtaattg
     7441 gaaataaagt aaaaaaagta tatgatgagg ttataaaaat tgtacctcaa tcaaataacg
     7501 taggtaaaaa gaatgaaagt caagaaaagc aaggtataat taattctata ttatcagcag
     7561 tagctggtat atttacacct acaatacctg ctatagcagg ggttggtatg attaaaggta
     7621 ttttatcagt tcttgctatg tattatatga ataaaaatgg agtagatata aaagaaacgc
     7681 aaagctatat tatcttaaat gccatggctg attctatatt ctattttatg cctattatac
     7741 taggatatac tgcggctaaa gtatttaatg caaataaaat aatatctatg gtattaggag
     7801 ctacactttg ttatccaact tttactgctt tgatggcagg tgaagaatct gttagatttt
     7861 taggattggc agtaacaaaa gctacgtata catcatctgt tattccaata attattgcta
     7921 tatgggctct ttcatacgtt gaaaaagtat tagaaaagta tatacctgag gtaattaaaa
     7981 taataatggt tccaacttta tcattggtag taatgttacc tgcaacatta tttttatttg
     8041 gaccaatcgg aatttatata ggaaatgtca taaacttttc ttataagtat atttatgaat
     8101 taagcccagc attgtgtgga gcatttgtag gtgggttatg gtgtgtgttg gttatatttg
     8161 gtgctcatag agcattatta ccaataggta taagtgatgt tgctcaaaca ggaagacaga
     8221 atttattggc atttgcagga gctgcaaact tttcacaagc aggggctgct ctaggagtat
     8281 tctttaaaac taaaaatcaa ggactaaaaa caatttctat gtctgcaact ataacagctt
     8341 tatttggtat aactgaacct gcaatatacg gtgccaatct tagattaaaa aagcctatgg
     8401 tatgtgccgt aatatgtggt gcatttggag gtgcgataat gggaatgggt ggagcttatg
     8461 gtaatgcatt tgcaaaccaa ggtgttttaa ctatacctgt atatgctgaa gcaggtgcat
     8521 taggtttctt aagttatcta ggtggttgtg ctatagcatt ttttggttca gctatttcaa
     8581 cttatctagt tggatttgaa gatttggaag agtcttctaa taaggaaaat tcatctataa
     8641 aagtagaaac gaaggatggt gttattgata taacttcacc tgtagagggc gaatgtatag
     8701 aattgtcaga agtaaaggat gatgtatttg cttcaaaagc tatgggagaa ggtatagctg
     8761 tgttacctac aaaaggagta ataacagccc ctactgattg tgaagtagct tctttatttc
     8821 caacattaca tgcaatagga ttaaaattag ataatggagc agaaatgtta atacatgttg
     8881 gaatcaatac agtagagtta aatggtaaat actttaccaa acatgttaat caaggagatt
     8941 tagtgaaaaa aggtgataaa cttatatcgt ttgatataga taaaataaaa aaggttggtt
     9001 atgatgtaac tacgccagtt atagtaaaca atacatttga ttttgggcaa gtagtttctt
     9061 gtaaatctag ttatgtttca accaatgata atataatttc attagtatta gctggtaatt
     9121 aagttaattt gactagagat aagtaaattg atttaaaagg aaataagtaa atcaacaatt
     9181 aaatacatct aaaagtaagt ttattgcaat gaattgttaa agaaaataat taatatggag
     9241 taaatctatt gcaatgaagt aacaaagaaa ataattaata tagagtaagt ttattgtaat
     9301 gaattgttaa agaaaataat taatataggg taaatctatt tcaatgaaat aacaaagaaa
     9361 agagttaata tagagtgaac ttattgcaat gaatcaacaa agaaaataat taataaaaag
     9421 gagaaatatt tatgaataca ggatttccaa aagacttttt atggggagca tcatcttctg
     9481 catttcaagt ggaaggagct tgggataaag acaataaagg aaaaaccgta gccgactata
     9541 attcttttaa aaagtctcat ttacaggcag atactaaagt cgcatcagac ttttaccata
     9601 attatgaaga agatattgaa ttaatgaaag aattagggat gaaaacatat cgtttttcaa
     9661 tttcatgggc aaggataata ccagatggtg aaggagagat aaaccagaaa ggtttagact
     9721 tttataataa gataatagac aaactaatag agtgtgatat tgagccattc gtgactttat
     9781 accattttga ccttccattt aaattagtag agaaatataa tggttgggaa agtagagaaa
     9841 ctgtctatgc ttttgaaaga tttgctaaaa tatgctttaa gcattttgga gatagggtaa
     9901 agtattggca acctcataat gaacagaatt taattgttag agtggaggag agaattaata
     9961 tatatgatga aacagattca tggaagatag ataaaatgcg tgctcagatg gattataatc
    10021 tttgcttagc acatgcatta gcagtcaatg catgtcatga gatgataaaa gaaagtaaaa
    10081 ttggagcagc agtatcatca tctgtaacat atcctcttac ttctaagcca gaagatatat
    10141 atgcagctag aatgaatgat aattttaaag tgtactatat gcttgatatg catcattatg
    10201 gagaatatcc agggtattat atgaagtatc ttgaaaaaag aaatatagtt cctcacatgg
    10261 aagatgggga taaagagata cttaaaaaag ctaaaatgga ttttatagct gtaaattatt
    10321 ataggacaaa ttgtgcagaa gctttacctg aagattcaca acatccattt ggattaagag
    10381 aaggaactgt tgattttagt atgtatggac tatttaaaat gtcaatgaat cctaacttag
    10441 aggcttctga gtatggagca gcaatagacc catcaggtct tagagttgcc ttaaatgaat
    10501 attggcaaag atatcactta cctgttataa taacagaaaa tggtctagga gctaaagata
    10561 tattagaaga tggaaaaatt catgatgact atcgtataga ctatttaaga agtcatataa
    10621 atgcatgtaa actagctatt gaagatggtg tagagatgat tggatattgt ccatggtcat
    10681 ttacagacct tttaagttct tctcaaggtt ttaataagcg ttatggactt gtttatataa
    10741 atagaactga ccatgaaatt ttagatttaa aaagaatcaa gaaagatagt ttttactggt
    10801 ataaagaagt tatagagaac aatggaatag taaaataaac ataaatatat attgagggtt
    10861 ttagtatgta cattacaaag gtattaaata attcgttgct acttgcaaag gatgataatg
    10921 atgaagaaat aatattgatg gggaaaggca ttggccataa ttataaggcc gggtctgagt
    10981 taaatgaaaa agatatagaa aagatttttg ttcttcatga tgaaaatata aaaaagagta
    11041 ttatacaatt ggcaacagaa atagatgcag aatattttgg tatagctcaa atgataattg
    11101 catatggaat agaaaaatat aacttaaaac ttatgaacca tatatatttg gccttaactg
    11161 accacattgc ctttgcagtg agacgtttta aagaaggtat aatgattgaa aatcactatt
    11221 tgttcgaaat caaggaattt aatccaaaag agtatgatat aggaaaatat gctattactg
    11281 tttttaagga ggttcttggt ttagacctac cagaagaaga gattggtaat atagcaggcc
    11341 attttataaa tgcacaacaa gataatcctt atagtgatag aaataagaga agtgctaaaa
    11401 ttgtaaatgc tattttgcaa atagttcatt atcacttttc tattgtatat aataaagagt
    11461 cattttatta taggagattt gtaatgcatc taaaggcctt ttcacaaaga tttctggcta
    11521 atgaaccatc aagagaaaaa atagatttta tatatgagca agttcaaaaa aattgtaagg
    11581 aagagtatga atgtgtgaaa aaggtaggag cttatatgta taaggaatac tccagagaat
    11641 taccaagaca agaggagtta tatttgatga ttcatattca taagatttta ggagaattat
    11701 gcgaaaatag ttagaatatc ttaatatgct agtaatatta taaaaaatta aaaattaact
    11761 gcactttaaa tatgaataat tgcattactt aagggggttg tcttaaaatt gattttttta
    11821 attaaatttg aagctcaact ccttttttaa acctttatat cattacaact ttaatgttta
    11881 tacgcctgta cttcagatgt atatctactt tttacatttc tcaacatgca aatctaccat
    11941 tctgtatata gtaaatgtat tatcatgttt gttttattca agcacactta taatacgctt
    12001 gattttttca caatgatgaa atatactaat gggtgttata ggtataagaa tcgcattaca
    12061 cttgaagaag taagtgataa atgagatata aatcctcata tgataaacta ctatttcatt
    12121 gaggaacata ttctaagtga tgtcaagata ctacaatatg actaaattcc tagtgataaa
    12181 aaagtctacg aatgagtgta gaaaggggaa ctaagatgaa taaagtttta attatagatg
    12241 atgataaaga tctttgtatt ttgttaaaaa agagtatttc aattgaacag attgaagcaa
    12301 actattgcca ttctgggaga gatggactgg ttgaactttc aaaacaagat tatcagttag
    12361 tagttctaga tgtgatgatg ccaggtatgg atggttttga aactatggaa caaatacgaa
    12421 aatatagcag tattcctatc ttaatgctga catcaaaaag tgataattac agcaaagtgc
    12481 atggattgcg agctggtgca gatgactatc taaccaaacc atttgaaatt acagaattta
    12541 ctgctcgtgt actgtcttta atccgacgtt acacccgttt taatccaaaa aatgaggagc
    12601 cacagcaact ttctttcaaa ggtttgttga ttgaccttga ccagcataca gttactacca
    12661 atggcgaacc tatgttgctt ccacccaagg aatttggtgt acttctttac tgtgcaaggc
    12721 atcagggtaa gattttgaca aaacagcaga tttatgaagc cgtgtggggt gagccatatg
    12781 tatatgatga cagcaatatc atggctatta tcagtcgcct aagaaaaaaa attgaactag
    12841 acccatcatc tcccatctat ttgcaaacaa taaaaggaat tgggtatcaa ttcaacacgg
    12901 aggtatgaaa atggatgaac aaacgataat tcttttctta ctcttgctgt cgattggtct
    12961 actttttctt tccatatttg ctttttccaa gcttgggcat attcacaaac gcttaaaaga
    13021 tatagaggag atactcgctg atgtacaaat tggagacgaa aatcgtaaaa tactaattaa
    13081 gccatgtgat gttatggctc cattggttta tcagcttaat gagattgtct acgattatga
    13141 aaataagtta ctttctctga aaaagtcgga taaagctagc aaacaactta tgacaagttt
    13201 atctcatgat gttcgtacac cattaacaac tctgattggt tatctggatg ctgtacacag
    13261 tggtatagtt atcgaacaag agcgggagga ataccttgaa atcgctaggc gtagagccta
    13321 cgacctcaag gattacattg atgttctttt tgactggttt cggttgaact ctgatgaatt
    13381 tacgctgtca atagaatctg tggaaattgc agagttgtca cgcaatattt tgaaagactg
    13441 gattcctata ttccatgaaa agaaactgaa ttttgaaatt gatattccag aaaatcgact
    13501 aatggtaaac ctcgacccag atggatattc ccgtgtggtc aataatctgg ttcaaaatgt
    13561 tcttgctcac agtaaggctc ggcaaatcaa gattaccatg agtgaggatt ccagaatggt
    13621 attactccgt gttgaggatg atggtgttgg gattgccaga gaaaatttac cacatatttt
    13681 tgagcgactg tataagtgtg ataaaggacg ttcagaaaaa ggaagtggtc ttggtctatc
    13741 tattgtcagt caaatggtgg agcgaatggg tggtcaggtt tccgtagaaa gtgagattgg
    13801 aaagtatacc gtatttacag tgagcttacc attaatataa aattgttttt cccccatgtg
    13861 acatgtatac acagggggat tttttgtaag ttataaagtt gcaaggttaa tacaaggttg
    13921 ttgcaaggtt aatgtaagat tgaggtggta tgataacttt aataaacaaa ggaggctttt
    13981 attatttatg aataatttaa ttattgaaac aaaaaatctc actaagcaat atggtgctca
    14041 aaagagcgtt tctaatttga atattcacgt tcagaaaggt cgcatttacg gattgctagg
    14101 aagaaacggt gcgggtaaga cgacaactat gaaaatgtta ttgggcctaa caaaaccaac
    14161 tactggagaa attaaaatat ttggtcagga cattcacaat aacgagaaga agcttcttcc
    14221 tcgaattgga tgtttgattg aatcaccagg gttttatcct aatctgacag gaacagaaaa
    14281 tctcaaaatc tttgcaaggc ttagaggaat accaaaaaaa aatgcagtag aatcagcact
    14341 ggatgtagtc ggtttgccaa acaatgacaa aaagttattt tctcaatact ctcttggtat
    14401 gaaacagagg ttggcgattg ctcttgccat tatgcatgac ccacaactgt tgattttgga
    14461 cgagcctatc aatggacttg atcctattgg tattgcagaa gtaagaacgt ttattcgtga
    14521 tctctgcaca gaacgaggta aaactatttt gatttccagt cacattcttt ctgaaattgc
    14581 cttgttggca gatgacattg gcattattga ccatggtatt ttgttggagg aagaaagtct
    14641 tgcccaatta gaagaaaaaa acagtaaata catacatttt gctgtatctg atgcagcaca
    14701 ggcagcaagg cttctggaaa cccaacttaa cctgaaaaat ttcaaagtag cagataatta
    14761 caatattatg ctatatgata cagatgtttc tgttgcagaa ataaatcgtg ctttcattct
    14821 aaacaacatt gaggtatcag aatctcattt atgcgaagat actcttgaag attacttcaa
    14881 gaaagtaaca gggggtgaag gaattgcttg atataatttc atgtgaattt tcaaaactca
    14941 agcggttaaa atttatactg atttctattt taggtgcttg cttatttcca attcccgcaa
    15001 caattttgat tgcaaaggac aacttgccct ttgagcaatt gttcaaatta gttgtcaatt
    15061 ttggatattt cttgctatta ccaattgtat taagtattgt tgctagccaa ctatttttta
    15121 tagaaagaga taatgatgta ttaaaaaatc ttgcaacagt gccagtgcca aaaggaaaac
    15181 tggctctggc aaaattggct gtgttgctgt ttatatcatt gttctactct gttgcagggt
    15241 taggagctac tatcattgga ggtttcattg ttggaatagt agagggggtt gctgtaaaat
    15301 taggaatgag cattgctttt ggaattatgt tttttgttac tgtacttcct gttgtggtac
    15361 tcattgtata tttcaatcgc agctatattt tttctattat actttctttt gtatatgcta
    15421 ttttcaattt tagtatatcc ttaaatatta tcaattttga accaaataac ccactattaa
    15481 gcgtattacc tgctcctgtt ataatgcgtt ggtggatggc attttgggga gatcccactg
    15541 gcgaatacac ggtacttaga cagccatatt tgctctcaac tcctgcttgt gtaggtattt
    15601 tatgtttgat aaccattata gcagtcctgc tgatttgtat gatatacaaa aagcaggaga
    15661 attaggaggt atctatgctt gatataatta ctacagaatt tcaaaaaata aaaagatata
    15721 atattctatg gattggtgtt gttgctgttc tgttttcagc actattggca gtttttcagg
    15781 ttgtaagttc acacggcagt gaccctctaa cgtatgagag ctttgcaaac ggagttatct
    15841 ggaataattt cagtcttagt tttccatttg ggattacact tattggagga tatcttatta
    15901 accgtgaata tacagaccaa accttaaaaa acatcctaac tgttcctatt tcattacgca
    15961 aattgctagt gggaaaatta attgcggttg gaggaattac tgtattgtta agtcttttca
    16021 gcttcttatg cacatttatt ttggggttgt tattttgcaa tatcaatata tctgttatac
    16081 tcgttgtaaa atccttagtt cagattttgt ctgttaatct gtattgttat gttgctgttt
    16141 tgccgattat tgcttatttt ggtcgtaaac aaaatgcatt tctgacgggt gtagggattg
    16201 catttgtata cggtttttgt ggtgtgtttg ttgctggaag aaatctgaca gacttttatc
    16261 caatcacagc aggtcttggt ctagttggat acaacaatgg tgctggtact gtttatcagc
    16321 cacttatcgg tgctatgact ttgattgtaa ttctaatctt gacaactatt ctacttgtat
    16381 ttacacctaa ttatgataaa gttatgacca tatctaagaa aaaagggata aagcataaaa
    16441 agtattctca taaataacat aaatattgtt tctattattt aagttgctag acagtgaaaa
    16501 gaaaaaatct gtcgtacagt ggctatatgc tcatgtattc aagaagctac agttttgatt
    16561 tttcataact gtagcttctt tttgtattta ttaaatcacg actatcataa tacttgcaat
    16621 aggtatagta acaagagaga gtactgtcat atgtgtttta tatattacat taagtatatt
    16681 ctaaaatata tgttttaaat aatatatttg aaaataggaa tatttaaata aaaaaaataa
    16741 tattatatat agttgacaaa aattggcaaa tgatatacta tataaatata gaaatagtaa
    16801 tcattactat tattaaaaag ggaggaatta attatgaact taaaaggaac taaaacagaa
    16861 aagaatttaa tggcaggatt tgcaggagag tcagaagcaa gaaataaata tacatactac
    16921 gcttcaaaag caagaaaaga aggttataat caaatagctg ctatatttga agaaactgct
    16981 aacaatgaaa aggaacatgc taaattatgg tttaaacttt tacatgatgg tatgccttca
    17041 acagaggaaa atttaaagga tgcagcagca ggtgaaaact atgaatggac tgatatgtat
    17101 gcaaaatttg ctaaagaagc tagagaagaa ggatttgaca agatagctta tttatttgaa
    17161 gctgttggaa aaatagagaa agagcatgaa gaaagatact taaagctttt agaaaactta
    17221 aatgaaggaa aaatatttaa aagagatgaa gaagttgttt ggcaatgtca aaactgtggt
    17281 catgtgtatg taggtactga agcaccagaa aaatgcccag tatgtgacca tccaaaagca
    17341 tattttaata taaaagctga aaattattaa aaattatcat tattaaaaag gaatgttaac
    17401 tatgaaattt tctaaacaac gagaactgat tttaaatgaa atattaaata atccggttca
    17461 tcctactgcg gattacctat atgaaaactt aaaaaaagat aatccgaatt taagcttagg
    17521 aactgtgtat agaaatttag ctcagctaac ggaacatggc tttataagaa aggttagtat
    17581 tccagggtat ccagatagat ttgatggtcg aatagataat cactatcata tcatatgtga
    17641 agtatgtgga gaggtatacg atttagaatc cgaagttctt aataatttac aagaattaat
    17701 atctgaggag acagacataa aaataacatc ttataacata agttttaaag ggatttgtaa
    17761 taattgtaaa aggtgtagcc aagtaggtta gttttcaaat tctaaaaata attaatgagg
    17821 tgagtttatg tgtagtgaac aaaaattttt tatatgtgag acttgtggaa accttgtagg
    17881 aatgatacaa agtggtggtg tacctatttt ctgttgtgga aaacctatga aggaacttgt
    17941 tcctaataca acagatgcag cagttgaaaa acatgtacct gtaattgaag tggatggaaa
    18001 taatgttact gttaaagtaa gtagcacaac tcatccaatg actaaagaac atcatatagc
    18061 atgggtgtat cttatgactg aacaaggtgg tcaacgcaaa tgtctagcag tagatggaga
    18121 acctgtagtt aaatttgctt tgaatgatga tgataaagtg atttctgcct atgcttactg
    18181 taatttacat ggattatgga aggctgaact gtaatattta aataaattga gtcaaaaagg
    18241 aggctataaa tgtattttca tacaaatagc ctcctataaa tttatataaa ttcaagtttt
    18301 aattttgttt aatgtcaata taatataagg agaaaataat atgtcaattt ataaatgtag
    18361 tgtttgtgga tatatttatg atgaatctaa gaatgataaa acatgggatg aattaagtga
    18421 agattgggaa tgccctgtat gtacaaaagg tcgtagttat tttggaaaga taagtactgt
    18481 ttactatgaa gaagatgaaa agatagcaga agacatagtt gaagatgaat ctaaattaaa
    18541 tactgaaaaa gagggagatt taaattactt aagtacatat ttaagaagag atgatgaagt
    18601 tgagaaacat atggatataa ttcacgaaat ggctgttaca ggaaaatcta taatagaacc
    18661 tatgagaact aaactaccag taatttcttg ggatgatatt ttaattatgg gtgctcaatt
    18721 aaatccatta cctttaaatg agcatgatga agtaaataca acaacaataa taggaaaaaa
    18781 agcaaagaaa cctatgatta tagaaaatcc agtttatatt tcccacatgt catttggagc
    18841 tttgtctaaa gaactaaaaa tagctttagc aaagggtgca gcccaaaata agacagctat
    18901 gtgtagtggt gaaggaggta tattaccaga agagaaggaa gcttcataca agtatatttt
    18961 tgagtatgta ccaaataaat atagtgtaac tgaagaaaat cttaaaaatt ctgatgctat
    19021 agagataaaa attggtcaag gaacaaaacc tggtatggga ggtcatcttc ctggcgaaaa
    19081 agtaactgaa gaaatagcaa aagtaagaaa taagcctgta ggtcaagatg taataagtcc
    19141 atcttgtttt gaggaaatac aatccaaaga ggatttaaag aaacttgtag atgaacttag
    19201 agaagtttca gaagggcgtc caataggtgt taaaatttca gcaggtcata ttgaaaaaga
    19261 tatggaattt atagcttatg ctaaacctga ttttgtaact attgatggtc gtggtggagc
    19321 tactggagca agtccaaagt tgcttaaaga tgcaacttca attccaacaa tatttgctct
    19381 ttatcgtgct agaaaataca tagatacaca tggattagat atagatttgg ttataacagg
    19441 tgggcttcgt atatctactg attttgctaa ggcaattgct atgggggctg atgcagttgc
    19501 tattgcaagt tctgctctta tggcagcggc gtgtcaacaa tatcgtatat gtggttctgg
    19561 taagtgtcct gtaggagtag ctactcagga tgaggaattg agaaaaagat tacatataga
    19621 aaatagtgct aatagagtgg caaacttttt aaatgtaagt ttagaagaac ttaagacttt
    19681 tgctagaatt tctggtcata aagatataca tgatttaagt gttgatgatt tatatactgt
    19741 taactctgaa atatcaaatt atacaaacat acagcatgta tagattgaag tttgaaacta
    19801 ttagatttta gacagctata aaagtaaatg ataaatatat tttataaaag agagtgtctt
    19861 aaaatgaact ttttagttca taagtcactc ttttttgtat gaagccaaat atatttatca
    19921 ttttagcaat gaaaaagaat cttatctctt tattttgaga tagactcctt ctatttatga
    19981 aaattaaaga tattaaattg taaaatattc tgtgatgtcg tactattaaa ctaaagattc
    20041 tgtactttaa agtaaataaa aatttaagga gtgattatta tggaaagatt gaagatacaa
    20101 gttttagtcg acaataatac ttatattgat agatactttg ttggagaacc agcagtaagc
    20161 tattatattg agattgatgg aaataggatt ttgtttgact cagggtattc agatgtattc
    20221 atatcaaacg ctgaaaaatt aaatatagat ttaggaaatt taactcatgt tgttttttct
    20281 catgggcata atgaccatac aagaggtatc cagtttttag aaaataggta tgatttatct
    20341 acagttgaac ttatttcaca tcctaactgt tttataccta gaaaacatgg agagaaaagc
    20401 ataggagctc cattttctgc tgaggaaatt aaaaatattt ttatgtataa tcctaaggat
    20461 aagccattta atctaagcaa gaattgtgtg tttttaggtg aaataccttc tataaatgat
    20521 tttgaaaaaa gagctaaaat tggtaaatgt aaaataggag atttgtggga agatgattat
    20581 gttttagatg attctgctat agtttgtaaa actgataaag gtatttttat tgttacagga
    20641 tgttctcata gtggaatttg taatatagta gagtatgcta aaaaggtatg tggagatgat
    20701 agagttattg gtatactagg tggttttcat ctttttgaat tgaataatag gttagatagt
    20761 acaatacaat atttggataa agaagaaata agaatgttgt acccttgtca ctgtgtttct
    20821 tttaaggtaa aagcgaagat gaatgaaata ttgaatataa atgaagttgg agtagggctg
    20881 accataagta tatagtattt gaaagtatat aagatttaag ttgaaggtta aaaagatatt
    20941 ttctatagaa aatatctttt ttgttacgtt tattttaaga gaaatcaata tacttttata
    21001 tgaaatattg taaaaaaatt tatttttaat gagaatatat tttcattaat aaacaatttt
    21061 attatgtatt aataatgtta gaataaatat aaataaatca
//
LOCUS       DBGJFU010000068        21084 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.068, whole genome shotgun sequence.
ACCESSION   DBGJFU010000068 DBGJFU010000000
VERSION     DBGJFU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.068"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..369
                     /gene="tuf"
                     /locus_tag="ACOIAS_002281"
     CDS             <1..369
                     /gene="tuf"
                     /locus_tag="ACOIAS_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791980.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGZ2701989.1"
                     /translation="DNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEE
                     GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLR
                     FSIREGGRTVASGVVATIIE"
     gene            741..1052
                     /gene="rpsJ"
                     /locus_tag="ACOIAS_002282"
     CDS             741..1052
                     /gene="rpsJ"
                     /locus_tag="ACOIAS_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGZ2701990.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1144..1773
                     /gene="rplC"
                     /locus_tag="ACOIAS_002283"
     CDS             1144..1773
                     /gene="rplC"
                     /locus_tag="ACOIAS_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGZ2701991.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1803..2426
                     /gene="rplD"
                     /locus_tag="ACOIAS_002284"
     CDS             1803..2426
                     /gene="rplD"
                     /locus_tag="ACOIAS_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGZ2701992.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2426..2716
                     /gene="rplW"
                     /locus_tag="ACOIAS_002285"
     CDS             2426..2716
                     /gene="rplW"
                     /locus_tag="ACOIAS_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGZ2701993.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2746..3576
                     /gene="rplB"
                     /locus_tag="ACOIAS_002286"
     CDS             2746..3576
                     /gene="rplB"
                     /locus_tag="ACOIAS_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGZ2701994.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3611..3892
                     /gene="rpsS"
                     /locus_tag="ACOIAS_002287"
     CDS             3611..3892
                     /gene="rpsS"
                     /locus_tag="ACOIAS_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGZ2701995.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3924..4259
                     /gene="rplV"
                     /locus_tag="ACOIAS_002288"
     CDS             3924..4259
                     /gene="rplV"
                     /locus_tag="ACOIAS_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGZ2701996.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4282..5097
                     /gene="rpsC"
                     /locus_tag="ACOIAS_002289"
     CDS             4282..5097
                     /gene="rpsC"
                     /locus_tag="ACOIAS_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGZ2701997.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            5133..5564
                     /gene="rplP"
                     /locus_tag="ACOIAS_002290"
     CDS             5133..5564
                     /gene="rplP"
                     /locus_tag="ACOIAS_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGZ2701998.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5566..5769
                     /gene="rpmC"
                     /locus_tag="ACOIAS_002291"
     CDS             5566..5769
                     /gene="rpmC"
                     /locus_tag="ACOIAS_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGZ2701999.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5793..6047
                     /gene="rpsQ"
                     /locus_tag="ACOIAS_002292"
     CDS             5793..6047
                     /gene="rpsQ"
                     /locus_tag="ACOIAS_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGZ2702000.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            6073..6441
                     /gene="rplN"
                     /locus_tag="ACOIAS_002293"
     CDS             6073..6441
                     /gene="rplN"
                     /locus_tag="ACOIAS_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGZ2702001.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6465..6770
                     /gene="rplX"
                     /locus_tag="ACOIAS_002294"
     CDS             6465..6770
                     /gene="rplX"
                     /locus_tag="ACOIAS_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGZ2702002.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6800..7342
                     /gene="rplE"
                     /locus_tag="ACOIAS_002295"
     CDS             6800..7342
                     /gene="rplE"
                     /locus_tag="ACOIAS_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGZ2702003.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7360..7545
                     /locus_tag="ACOIAS_002296"
     CDS             7360..7545
                     /locus_tag="ACOIAS_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HGZ2702004.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7578..7976
                     /gene="rpsH"
                     /locus_tag="ACOIAS_002297"
     CDS             7578..7976
                     /gene="rpsH"
                     /locus_tag="ACOIAS_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGZ2702005.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            8007..8549
                     /gene="rplF"
                     /locus_tag="ACOIAS_002298"
     CDS             8007..8549
                     /gene="rplF"
                     /locus_tag="ACOIAS_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGZ2702006.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8585..8953
                     /gene="rplR"
                     /locus_tag="ACOIAS_002299"
     CDS             8585..8953
                     /gene="rplR"
                     /locus_tag="ACOIAS_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGZ2702007.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8974..9483
                     /gene="rpsE"
                     /locus_tag="ACOIAS_002300"
     CDS             8974..9483
                     /gene="rpsE"
                     /locus_tag="ACOIAS_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGZ2702008.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9498..9683
                     /gene="rpmD"
                     /locus_tag="ACOIAS_002301"
     CDS             9498..9683
                     /gene="rpmD"
                     /locus_tag="ACOIAS_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGZ2702009.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9716..10159
                     /gene="rplO"
                     /locus_tag="ACOIAS_002302"
     CDS             9716..10159
                     /gene="rplO"
                     /locus_tag="ACOIAS_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGZ2702010.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10203..11471
                     /gene="secY"
                     /locus_tag="ACOIAS_002303"
     CDS             10203..11471
                     /gene="secY"
                     /locus_tag="ACOIAS_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGZ2702011.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11488..12138
                     /locus_tag="ACOIAS_002304"
     CDS             11488..12138
                     /locus_tag="ACOIAS_002304"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGZ2702012.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12139..12885
                     /gene="map"
                     /locus_tag="ACOIAS_002305"
     CDS             12139..12885
                     /gene="map"
                     /locus_tag="ACOIAS_002305"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGZ2702013.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12907..13176
                     /locus_tag="ACOIAS_002306"
     CDS             12907..13176
                     /locus_tag="ACOIAS_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HGZ2702014.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13214..13432
                     /gene="infA"
                     /locus_tag="ACOIAS_002307"
     CDS             13214..13432
                     /gene="infA"
                     /locus_tag="ACOIAS_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGZ2702015.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13468..13581
                     /gene="rpmJ"
                     /locus_tag="ACOIAS_002308"
     CDS             13468..13581
                     /gene="rpmJ"
                     /locus_tag="ACOIAS_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGZ2702016.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13734..14105
                     /gene="rpsM"
                     /locus_tag="ACOIAS_002309"
     CDS             13734..14105
                     /gene="rpsM"
                     /locus_tag="ACOIAS_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGZ2702017.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14141..14539
                     /gene="rpsK"
                     /locus_tag="ACOIAS_002310"
     CDS             14141..14539
                     /gene="rpsK"
                     /locus_tag="ACOIAS_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGZ2702018.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14571..15194
                     /gene="rpsD"
                     /locus_tag="ACOIAS_002311"
     CDS             14571..15194
                     /gene="rpsD"
                     /locus_tag="ACOIAS_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGZ2702019.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15272..16219
                     /locus_tag="ACOIAS_002312"
     CDS             15272..16219
                     /locus_tag="ACOIAS_002312"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGZ2702020.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16240..16581
                     /gene="rplQ"
                     /locus_tag="ACOIAS_002313"
     CDS             16240..16581
                     /gene="rplQ"
                     /locus_tag="ACOIAS_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGZ2702021.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16701..17534
                     /locus_tag="ACOIAS_002314"
     CDS             16701..17534
                     /locus_tag="ACOIAS_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGZ2702022.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17522..18388
                     /locus_tag="ACOIAS_002315"
     CDS             17522..18388
                     /locus_tag="ACOIAS_002315"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGZ2702023.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18382..19185
                     /locus_tag="ACOIAS_002316"
     CDS             18382..19185
                     /locus_tag="ACOIAS_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGZ2702024.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19217..19948
                     /gene="truA"
                     /locus_tag="ACOIAS_002317"
     CDS             19217..19948
                     /gene="truA"
                     /locus_tag="ACOIAS_002317"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGZ2702025.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            20068..20499
                     /gene="rplM"
                     /locus_tag="ACOIAS_002318"
     CDS             20068..20499
                     /gene="rplM"
                     /locus_tag="ACOIAS_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGZ2702026.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20528..20920
                     /gene="rpsI"
                     /locus_tag="ACOIAS_002319"
     CDS             20528..20920
                     /gene="rpsI"
                     /locus_tag="ACOIAS_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGZ2702027.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8425 a         2493 c         4409 g         5757 t
ORIGIN      
        1 gataatatag gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta
       61 ctagcaaaga ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt
      121 aaaaaagaag aaggtggaag acatacacca ttctttgatg gatatagacc acaattctat
      181 ttcagaacaa cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg
      241 cctggagata acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga
      301 ttaagattct caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata
      361 atagagtaat cctttactaa actaatggat ttaatataaa aaaagagggg ggtaactcct
      421 ctcttttttg ttttttgttg acaagtagcg ctaaagcata tatactaaac tagtgccttg
      481 agacagatta acaatcaagt ttaaggttat gatgttgtaa tagaaaataa aattgaaaaa
      541 aacagaaaaa aaacttgaaa aaatgtaaca gatgatataa aatataatag tgtgcttgag
      601 agattaagaa aataataatt aaatcctagt gaaatacgaa ctgatatcga tttaaaagtt
      661 acaacacacc cggagcagtg tatcggtatt ggttgtacgc aacttgaaaa ttaaacacgt
      721 gataaaggag ggaaaacaaa atggctaaga atgaaaaaat aagaataaga ttaaaatcat
      781 atgatcacaa attattagat ttttcagcag gtaaaatagt tgaaactgct aaaaaggctg
      841 gatcacaagt ttcaggacct gtgcctctac caacagaaaa acaagttgta actatattaa
      901 gagctgtaca taagtacaaa gactctagag aacaatttga aataagaact cataaaagac
      961 taatcgacat agctaatcca acacctaaga cagttgactc attaatgaga ttagacttac
     1021 cagcaggtgt agatatagaa ataaaattat aagaacccct aagatattat cctatagatg
     1081 attatcttag aatgattact ctaaaagatg taatccgctg taagaattat aggaggtgct
     1141 aatatgaaag gaatattagg caaaaaagtt ggtatgactc aaatattcac tgataaaggt
     1201 gtagttatac ctgtaacagc tgttgaagca gggcctatgg tcgttactca aataaaaaca
     1261 gttgataaag atggatacaa tgctatacaa ataggttttg aagatgctaa agaaaaagct
     1321 ttaaacaaac ctaaaaaagg acatttagct gctgctaatg ttttaaagaa acatttaaaa
     1381 gaatttagag tagattctgt agaaggatac acagtaggac aagaaataaa agctgatgta
     1441 tttgaagcag gtgcaaaaat agatgttact ggaataagta aaggtaaggg attccaaggt
     1501 ccaataaaaa gacatggaca aagtagaggt cctgaaactc acggttctag ataccacaga
     1561 agaccaggtt caatgggagc ttgttcttac ccaggtagag tatttaaaaa caaaaaatta
     1621 gctggacata tgggaagcgt aaaggtaaca gttcaaaact tagaggttgt taaagtagat
     1681 gctgacaaga accttatatt agttaaagga gctataccag gagctaaagg ttcagtagta
     1741 actataaaag aagctataaa ggtttctaaa taatgactaa cctagagaaa ggaggaataa
     1801 caatgccaaa attaaatgta ctaaatgtta gtggacaaaa cgttggagaa atagaattat
     1861 cagattctat atttggcgta gaagttaatg gacatgtttt atacgaagtt gtaaaaaatc
     1921 aattagcaaa taagagacaa ggaactcaat ctgctaaaac tagagcagaa gttagaggtg
     1981 gcggaagaaa accttggaaa caaaaaggaa caggtagagc aagacaaggt tctacaagat
     2041 ctgtacaatg ggtaggtgga ggagttgctt ttgcacctaa accaagaagc tacaaatata
     2101 cattacctaa gaaggtaaga agattagcta tgaagagtgc tttatcttca aaagttcaaa
     2161 atagtgaagt tatagtatta gatgcattaa acatggatgc tcctaagact aaagaatttg
     2221 ctcaaatatt aaacaatata aatgctgcta aaaaagcttt agtagttata gctgacaaaa
     2281 acgataacgt aataaaatca gctagaaata tagaaggtgt tcaaactgca ttagttaata
     2341 ctatgaatgt atatgatata ttaaaatacg attcatttat aataactaca gatgctgtta
     2401 aaaaagtgga ggaggtgtac gcataatgac taatccacat gatgtaataa taagaccagt
     2461 tgtaactgag cacagtatgg ctgaaatggg tgaaaaaaaa tacacttttg ttgtggctaa
     2521 agatgcgaac aaaactgaaa taaaaaaagc agtagaaaaa gtattcggag ttagtgttga
     2581 taaagtaaat actttaaact acgatggaaa agttaaaaga atgggtagaa ctcaaggaag
     2641 aacatcaagc tttaagaaag cagtagttaa attaactgct gatagtaaag aaatagaatt
     2701 cttccaagga atgtagtatt ataacaaacg aaggagggaa aacagatggc tataaaaaag
     2761 tttagaccaa cttctcctgc cttaagacaa atgacagttt tagtttctga tgaaataaca
     2821 tgtaatcaac cagaaaaatc tcttttagtt aacttaaaga aaaatgctgg tagaaacgta
     2881 catggtagaa taactgttcg tcatagaggt ggaggacaaa aaagaaaata tagaataata
     2941 gattttaaaa gagataaaga tggtatacct gctaaggttg ctactataga atatgatcca
     3001 aacagaactg caaatatcgc attattaaac tatgctgatg gagaaaaaag atatatatta
     3061 gctccagttg gaattaatgt tggagatact atattatcag gattaggtgc tgatataaaa
     3121 ccaggaaact gtttagcatt aaaagatatg ccagttggta ctatcattca taatatagaa
     3181 ttaaagccag gaaaaggagc tcaattagtt agatctgctg gagtttctgc acaattaatg
     3241 gctaaagaag gaaaaaatgc tttattaaga ttaccatcag gtgagatgag attagtaagc
     3301 ataaactgta aagctactat aggtcaagtt ggaaatatag agcatggtaa cgtagttatc
     3361 ggtaaagcag gtagaaaaag acatatggga ataagaccta ctgtaagagg ttctgtaatg
     3421 aaccctaatg accatccaca cggtggaggg gaaggtagat ctccaatagg tagaccatct
     3481 cctgttactc catggggtaa accagctctt ggatacaaaa ctagaaagaa aaataaagct
     3541 tcaaataaac taatagtatc aagaagaact aagtagtaaa tttatttttg ctcggaaagg
     3601 aggaatataa atgtcaagat caactaaaaa agggcctttt gtccatgcaa gacttttaaa
     3661 gaagatagaa gctatgaatg ctagtggaaa taaagaagtt ataaaaactt ggtcaagatc
     3721 ttcaactgta tttccacaaa tggtagaaaa cactatagct gttcatgatg gaagaaaaca
     3781 tgttccagta tatataacag aagatatggt tggacataaa ttaggtgagt tcgttcctac
     3841 tagaactttc aaaggacata aggatgacga aaaatctaat aagagaaaat aattaaatta
     3901 tgactaagaa aggaggaata acaatggaag caaaagctac tgctaaatac gtacgtgtat
     3961 caccaagaaa agcaggccaa atatgtgacc ttgttagagg aaagaatgtt gatgaagcat
     4021 tagcaatatt aaagtttact ccaagaggag cagcttcaat aatagctaag gttgtaaaat
     4081 cagctaaagc taacgcagaa aacaatcacg aaatggatac tgaaaaatta tatatagcat
     4141 caatagttgc taatcaagga ccaacaatga agagattcat gcctagagct atgggtcgtg
     4201 caactacaat aagaaaaaga acttctcata tagaggttgt tgttaaagaa aaaaaataat
     4261 taatagatag gagggaaagt aatgggtcaa aaggttaatc cacacggctt aagggtcggt
     4321 gttataaaag attgggactc aagatggttc gctactgata aaaaagagtt cggaaactta
     4381 ttattagaag accataatat acgtaaattc ttaaagaaaa gattatactc agctggagtt
     4441 gctaagatag aaatagaaag atcagctaac aaaataaaaa tggacttaca cgttgctaag
     4501 cctggtgtag ttataggaag agctggtgca ggaatcgaag cattaaaagc tgaattagaa
     4561 aaaatgacaa agaaaactat aatagttaat atagtagaag taagaagtac agataaaaat
     4621 gctcaattag tagctgaaaa tatagcttta gctatagaga gaagggttgc tttcagaaga
     4681 gcaatgaaac aagctataca aagagcaatg aaatctggtg ctaaagggat aaaagtttct
     4741 gcttcaggaa gattaggtgg agctgaaatg gctagaactg aaggttatag tgaaggaaat
     4801 gtgccactac aaactttaag agcagatata gattatggtt tcgctgaagc tgatacaact
     4861 tatggaaaaa tcggtataaa agtttggata tgtaatggtg aagttttacc aactagagac
     4921 ggtgtaaatc caagagaaga aagcagaaag agcgatagaa gagacaacaa gagagataat
     4981 agaagaaacg atagaagagg aaatgacaga agaggaaatg ataacagagg aaactacaga
     5041 ggacaaagac ctcaaggtgg atcaagacct caaagaactg aaaacaaagg aaattaataa
     5101 tagttaggtt caaggaagga ggaaaaatac tcatgttaat gccaaaaaga gtaaagcgtc
     5161 gtagagttca tagaggaagc atggctgggc aagctcataa gggtaataaa gttacttatg
     5221 gtgagtttgg attagtagca ttagaagctt cttggataac ttctaatcaa atagaagctg
     5281 ccagaatcgc gatgactaga tatataaaaa gagggggaaa ggtttggata aaaatattcc
     5341 ctcataaacc agtaacaaga aaaccagcag agactcgtat gggtgcaggg aaaggttctc
     5401 cagaatattg ggtagctgta gttaagccag gaagagttat gtttgaatta gcaggtgttt
     5461 ctgaagataa agcaagagaa gctatgagac ttgctgcaca taaattacca atcaaatgta
     5521 aatttgttaa aaaagaagat ttagaagtaa agggtggtga ataggatgaa agctaaagaa
     5581 ttaagagatt taacaagcga agagctaatg aacaaattaa acgacttcaa aagtgaatta
     5641 tttagcttaa gattccaatt agctactggt caattagaaa atacagctag aataaagttt
     5701 gttaagaagg atatagcaaa agttaaaact gttcttgctg aaagaaagtt atacgaaact
     5761 agagcttaat ttggaaagga ggctttttaa acatggaaag aggaagaaga aaagttagaa
     5821 taggccgtgt tgttagtgat aagatggata aaacaatagt agtagctgtt gaagaattcg
     5881 tacgtcatcc actatataat aaacgtgtta agagaactaa gaaattcaag gctcatgatg
     5941 aaaagaatat atgtaacatc ggagatagag ttaaaataat ggaaactaga ccattatcta
     6001 aagataagag attcagacta gttgaagttg ttgagaaagt taagtagttt ttgaagaagg
     6061 agggattacg atatgataca acaagaatca cgtctaagag ttgctgataa ctcaggagct
     6121 aaagaacttt tatgtatacg tgttctaggc ggaagtaaaa gaagatatgg taacataggc
     6181 gacgttatag ttgctactgt taaaagtgca acacctggtg gagttgtaaa aaaaggtaag
     6241 gttgttaaag ctgttatagt aagaagcaaa caaggcgtaa gacgtaatga cggaagctat
     6301 atatcttttg atgaaaatgc tgctgttata ataaaggacg ataaaactcc agtaggaact
     6361 cgtatattcg ggcctgttgc tagagagtta agagacaatg aattcatgaa aatagtttct
     6421 cttgctccag aagtactata attaaggagg tgcaatagga catgatgcgt gttaaaaaag
     6481 gtgacactgt tgtagttata gcaggtaaag ataaaggtaa aaaaggttca gttttaaagg
     6541 tatatcctaa aactagcaaa gtgttagttg aaggtgtaaa tgtaataact aaacatcaaa
     6601 aaccaagtgc tatgaaccaa caaggtggaa taataaataa agaagcccca atacacatat
     6661 ctaatgtaat gccatttgat cctgaaacag gaaaaggcgt tagagtaaga tatgaagtaa
     6721 aagatgggaa caaagtaaga gtatcagcaa agagcggaaa agaattataa taaatattgc
     6781 ttgaaaggag ggaccttaaa tggcttctag attacaagaa aaatacatga aagaagttgc
     6841 tcctgcttta atggagaaat ttggatacaa aaacgtaatg gagataccta agttaaataa
     6901 aatagttatt aacatgggta taggtgacgc tagagaaaat ccaaaaggat tagaaaaagg
     6961 cgttgaagaa ttagaaatga tatcaggaca aaagcctgtt ataactaaag ctagaaaatc
     7021 tgttgctaac ttcaaattaa gagaaggaat gccaatagga acaaaagtta cattaagagc
     7081 tgacaaaatg ttctacttca tggacaaatt agtttcagtt tctttaccaa gagttagaga
     7141 ctttagagga gttaacccta atgcttttga tggtagagga aattatgctt taggagttaa
     7201 agaacaatta atattccctg aaatagaata tgataaaata gataaagtaa gaggaatgga
     7261 tataatattt gttactacag caaaaactga cgaagaagct cgtgaattat taaaattatt
     7321 aggaatgcca ttttctaagt aagcaaagga gggattcccg tggctagaaa agcgatggtt
     7381 gttaagcaac aaagaaagca aaagtacgct actagagaat acactagatg tactatatgc
     7441 ggaagaccgc attcagtttt gaaaaaattt ggtatatgcc gtatatgctt tagagaatta
     7501 gcttataaag gtcaaatacc tggtgttaga aaagcaagct ggtagaagct tgattagaat
     7561 ggaaggaggg caacaaaatg acaatgacag atccaattgc agatatgctt actcgtataa
     7621 gaaatgctaa tgttgttaag catgaaactg tagatgttcc agcttctaat atgaagaaag
     7681 aattagctag aatcttatta gaagaaggtt tcataagagg ttatgatgtt atagaagatg
     7741 gaaaacaagg tatcataaga atacaattaa agtacggaca agaaggcgaa agagttataa
     7801 caggtcttaa gaaaatatct aagcctggaa tgagagttta cgctgctaac catgagatac
     7861 caaaagtatt aaacggatta ggaatatcag ttatatctac ttcaaaagga atattaactg
     7921 acaagcaagc tagaaaagaa aatgttggtg gagaagtaat ctgctacgtt tggtaattaa
     7981 tatgaacaat gtaaggaggt gcgaccatgt caagaatagg tgttaagcca ataataatac
     8041 cagcaggtgt tgaagttact atagctgaag gaaatttagt tacagtaaaa ggtccaaaag
     8101 gaactttaac taaacaatta agtgctgagt taaacataaa aaaagaagaa aatacaataa
     8161 tggttgaaag accaactgat aataaaaaac atagatcttt acacggacta actagaactt
     8221 tattagataa tatggttgta ggtgtaaata ctggtttcga aaagaaatta gaattaaaag
     8281 gtgttggata tagagctcaa aaacaaggaa agaaattagt tatgaactta ggtttctctc
     8341 atccagttga gatggaagat ccagaaggaa taactgttga agctccaaac caaacagagt
     8401 taatagtaaa aggtatagac aagcaattag tagggaatta tgctgctaag ataagagctt
     8461 ggagaaagcc agagccatac aaaggtaaag gtataaaata cgttgatgaa gtaatcagac
     8521 gtaaagaagg aaaaactggt aaaaaataat accaaaactt aaacgctaga aaggagtgag
     8581 ctaggtgtta aaaaaggctg ataaaaatgc taatagactt caaagacata agagagttcg
     8641 tagaaaaata agcggtactt ctcaaagacc aagattatgt gtatttagaa gtgctaacaa
     8701 tatatatgct caaataatag atgacacaaa aagagtaact ctagttgctg catcttcttt
     8761 agaagcagaa gttaagagtg ctgtcaatca tacaggaaac aaggaagcag ctaagaaagt
     8821 tggagaatta gttgctaaaa aagctgttga aaaaggaatc actgaagttg tatttgacag
     8881 aggtggatac ttatatcatg gaagaattca agaattagct gaaggtgcta gagaagctgg
     8941 tcttaagttc taatacgaag gagggaaaaa tacatgctac gtcgtaagcc gatagatgca
     9001 ggacaacttg acttacaaga aaaagttgtt gaagtaagac gtgttactaa ggttgtaaaa
     9061 ggtggtagaa atttcagatt tgcagcatta gtagttgttg gagatgaaaa cggacacgtt
     9121 ggtataggtg ctgggaaagc aatggaagta ccagacgcta taaaaaaagc agttgaagat
     9181 gctaagaaaa atctaatagt agtgccaata gttgggacta caattcctca cgaagtacgc
     9241 ggacactttg gtgctggaaa tatattaata atgcctgctg tagaaggtac aggagtaata
     9301 gctgggggac ctgctagagc cgtacttgag ctagctggac taaaagacgt tagagctaaa
     9361 tctttagggt ctaacaatcc aagaaatatg gtaaatgcta caatagaagg acttaattct
     9421 ctaaaaacag ttgaagatat agctaaactt agaggtaaaa aagttgaaga acttctaggg
     9481 taaggaggta gtgtaaaatg gctaaattac aaataaagtt agttagaagt gttataggaa
     9541 ctactcctaa ccaaaaaaag aatgttgaag cgttaggatt aagaaaaaga gaacaagttg
     9601 ttgtaaaaga agacaatgct caaacaagag gtatgataaa taaagtaagt cacttattag
     9661 aagtaactga aatagctgaa taatttttat aaagtagtgc aaggaggtgc aatccatgaa
     9721 gttacatgag ttaaaacctg ctgaaggtgc agtaagagct aagagaagat taggtagagg
     9781 tactgcaact ggacaaggta aaactgcagg acgtggacaa aaaggtcaat ggtctcgttc
     9841 tggtggtgga gtaagagtag gatttgaagg tggacaaatg ccactagcta gaagacttcc
     9901 taagagaggt ttcaataaca tatttaagaa agtttacaca gaagttaatg ttgaagtttt
     9961 aaacagattt gaaaatggaa cagaaataac tgcagaatta ttaaaatcta ctaaaactat
    10021 aagcaaaata ggtaaagacg gaattaaaat cttaggtgaa ggtaatttag agaaagcttt
    10081 aactgttaaa gctgctaaat ttacagcttc agctcaagaa aaaatagaaa aagctggtgg
    10141 aaaagcagag ttagtataat aacctgctaa tctcctagcc agtacaggcg gggtgaatta
    10201 gcgtgctgtc aaaattaaaa caagcttgga agattaaagc agtaagaaaa aaagtaatgt
    10261 atacacttat gatgatcgtg atattcagaa taggtactac tataccagtt ccaggcatag
    10321 acactagtat tatacaaaag atggtaggcg gcaatagtct actttctctg tataatatgt
    10381 ttactggtgg agcatttagt aacttttcac tatttgcttt aggtataagt ccatatatca
    10441 ctgcatcaat cataatacaa cttctaacag taggttttga aagtttagca gaacttcaaa
    10501 aatctggtga agaaggaaag aaaaaaatca ataagtacac taaatataca gcactagctc
    10561 tagcagttgt acaagctttg ggaattacat taggaattgt aagaagtgct ttaatatcaa
    10621 atagtgtgtt ttttatcaca actgttgtaa taacattagt ttcagcaagt atgttagtaa
    10681 tgtggattgg ggacaaaatt actgaaaaag gaatcggtaa tggtagttca gtaataatat
    10741 ttgcaggtat aatatcaaga atacctacag atgttattaa aatatcgcaa caagttaaat
    10801 ctggcgaagt tgcaccttgg gttatagtga ttctagctgt agttatatta cttacagtaa
    10861 caggagttac atttatacaa gaagcaacta gaaagatacc tgtacaatat gctaaaagag
    10921 ttgttggaag aaaaatgtat ggaggacaaa gttctcatat accaatgaaa gttaaccagt
    10981 caggagttat gcctataatt tttgctagtt cacttttagc tttccctcaa acaatagcta
    11041 tgtttatggg tccaaatgct caggcttttg ttcaaaaata cttaagtatg gcaacagaac
    11101 aaggattttg gacatataga tcaattgaga ttctacttat aattttcttc tcatattttt
    11161 atacaactgt atcatttaat acagaagaca tatctaaaaa tatgaaaaat aatggtggat
    11221 ttataccagg tataagacca ggtgagccta ctatggatta tttaaataga attttaacta
    11281 gattaacttt agctggagct actttcttag caataattgc tatggtgcca gctcttacaa
    11341 cacattatat gaaagtaaat atgagtctag ctgggacttc attgctaatc gttgttggag
    11401 ttgctttgga gcttaagaga cagttagagt caaacttagt tatgagaagc tatcaaggtt
    11461 tcttaaaata aaatggagat ggataatatg agaataatat tacttggacc tccaggtgct
    11521 ggtaaaggta ctcaggcggc aggaatagta gaaaaataca atatacctca tatatcaaca
    11581 ggagatatat tcagaaagaa tataaaagag ggaacagaac ttggaaaaaa agctaaagaa
    11641 tacatggacc aaggtttatt agtaccagat gagttaactg taggtttagt tactgataga
    11701 atatctcaag aagattgtaa aaatggattt atgttagatg gatttccaag aaatgtagca
    11761 caaggagaac atttagatat cttcttaaaa aatgctggta tatcactaga taaagttgtc
    11821 aatattgaag ttgataagag tatattagtg tctagagcag ttggtagaag aatatgtaag
    11881 tcttgtggag ctacttacca tgttgagttt aatcctccta aagtagaagg tgtatgtgat
    11941 gtatgccaag gagaattata tcaaagagct gatgataatg aagaaactgt atctaagaga
    12001 atacaagttt atctagatga aactaagcct ttagtagatt attatagcaa acaaggtata
    12061 atagcagata taaaaggtga tcaagcaata gataaagtat ttgaagatat tgtcgcagcc
    12121 ttaggaagtg gaaaataaat gattatcttg aaatcaaaaa aagaaataga acttttaaga
    12181 gaagcaggta aaatagttgc tgacactcat gaagttttaa gaaaagctat ttctccggga
    12241 atatctactt tagaattaga taaaatagct gaagagaata taagaaaata caatgcagaa
    12301 ccttctttta aaggatatgg aggttttcca ggaagtatat gtgcttctat caatagagag
    12361 gtagtacatg gtattccagg agaaactata ttacaagaag gtgacattgt aagtttggat
    12421 ataggagcct attataaagg atatcatggt gattctgcga aaactcacgg agtaggcatg
    12481 atatctgaag aagataggaa attgatagaa gtaactagag aaagcttcta tgaaggaata
    12541 aaatttgcta aattaggata tagactttca gatatctcac acgcagtaca aacacacgtt
    12601 gagaaacatg gtttctcggt agttagagat ttagtgggtc atggagttgg agcaaatctt
    12661 catgaagacc cacaagtacc taattatgga ctcccaggaa aaggaccaag acttagagaa
    12721 ggaatggtta ttgctataga gccaatggtt aatgcaggtc gttatcatgt aaagactcta
    12781 tcagatgggt ggacgactgt tacaatagat ggaaaaaaat cagctcatta tgagcatacg
    12841 atagcaatta ctgaacatga acctttgata ttgacaaagt tgtaatttaa aaagtaggtg
    12901 aaaaaagtgc tatcagataa tttaagtata ggtcaagttg ttaaagtttc attaggacga
    12961 gataaaggaa atttattctt tgtagtcaaa ataattaata atgaatatgt attaatagct
    13021 gatggaaaaa agagaaaact tgacaaacct aagttaaaga aagtgaagca cttaaaaaaa
    13081 tataatttta ttaatgatga agttagaaaa agagtagtat ctggacaaga gattacagat
    13141 tcttttttaa gagctgaact tactaagtta aattagataa tttaacttat tgaaatggag
    13201 ggctggttag ttaatggcca aaaaagatgt tatagaatta gaaggtacag tttcagaagc
    13261 tttacctaat gctatgttca aagttaaact agaaaatgga catgagatat tatgccacat
    13321 ttctggaaag ctaagaatga acttcataag aattcttgaa ggtgacaagg tgaatgttga
    13381 actttctcca tatgacctta caagaggaag aattacttgg cgtaagaagt agactatatt
    13441 aatatttagt ctaaggaggg attaataatg aaggttagac catcagtaaa accaatatgt
    13501 gaaaaatgta aagtaataaa aagaaaagga aaagtaatgg ttatctgtga aaatccaaag
    13561 cataagcaaa agcaaggata agaaattatt actagcattt tgttaaattt tatagtataa
    13621 tactatattg tgatgttaac aaaagaacgt taaaatcgtt gaacaatctg gcgtaggatt
    13681 ggcatatgat gtgaagtttt aaacactaaa aactatgtgt aggaggtgcg atcatggcaa
    13741 gaatagctgg ggtagattta cctagagaaa aaagagcgga gataggctta acttatatat
    13801 atggtatagg taaagcaact gctaacgaaa tattagctaa ggctgagata aatccagata
    13861 ctagaataaa agatttatca gaagatcaag ttaatgagtt aagaaaagtt atagatgacg
    13921 atttcttagt tgaaggtgac ttaagaagag aaattgcttt aaacataaag agattaagag
    13981 atataaaatg ttatagagga ataagacacg ctaaaggtct tccattaaga ggacaaagaa
    14041 ctaagactaa tgctagaact agaaaaggtc ctagaaaaac agtatctcgt aaaaagaaaa
    14101 aataaataaa taataactag atgaaggagg gaaaaggaaa atggctaaac caaaaaagaa
    14161 agttacacgt attagaagaa gagaacgtaa aaatatagaa cgtggtcatg ctcatataca
    14221 atcaactttc aataatacaa taataacttt aactgacgtt cacggaaatg ctatatcttg
    14281 ggcaagttct ggacaattag gattcaaagg atcaagaaaa tcaactccat ttgcatctca
    14341 aatggctgct gaaacagctg caaaagctgc gatggaacac ggactaaaaa gtgttgaggt
    14401 attcgtaaag gggccaggtt caggaagaga agctgcaata agagctttac aagcaactgg
    14461 actagaagta actatgataa aagacgttac tccaatccca cataacggat gcagaccacc
    14521 aaagagaaga agagtgtaat taataagagt acaataggag gtgtagataa atggcaagat
    14581 atacaggtgc atcatgtaga caatgccgta gagagggaat gaaattattc cttaaaggtg
    14641 atagatgtta tacagacaaa tgtgctatag taaaaagaaa ctatgctcca ggtcaacatg
    14701 gccaaggaag aaagaaagtt tctaactatg gattacaatt aagagaaaaa caaaaagtta
    14761 aaagaatata tggagtttta gagactcaat ttagaaattt atatgaacgt gctgaaaata
    14821 tgcctggtaa agcaggtgag aacttattaa gtttattaga gagaagatta gataatgtag
    14881 tttacagaat gggactagca tcttctagaa aagaagctag acaattagtg actcatggtc
    14941 atttcacttt aaatggaaat aaagtagaca taccttcatt aatagttaag gttggagatg
    15001 ttatagaagt taaagagaaa tcaagatctt ctgcaaaatt caaaaattta gtagaagtta
    15061 actcaagaat agctcctaag tggttagaag ctaatgtaga aggaatgaca gcaaaagtag
    15121 ttggtgttcc aacaagagaa gatatagatc ttgagatagc agagcactta atcatagaac
    15181 tttactctaa ataataagca cacttttaaa aattatttta aaattttgtt taccctcagt
    15241 ggattactga attttaagga gggttttgtc catgatagaa atagaaaagc caaaagtaga
    15301 tatagttgag cttagcgaag actatagata tggtaagttt gttatagagc ctctagaaag
    15361 aggatatgga ataactatag gtaacgcatt aagaagaata ttattatcat cattaccagg
    15421 tgtagcagtg aatgctataa aaatagatgg agttcttcat gaattttcaa caatacctgg
    15481 tgttaaagaa gatgttactg agataatatt aactttaaaa gagctttcag caactataga
    15541 tggtgaagga agtagaacac ttaaaataga agctcaagga ccatgctcta tcacaggtgc
    15601 agatataatc tgtcctccag atgttgaaat attaagtaaa gatttagcaa tagctacatt
    15661 agatgataat gctaaactta atatggagat atttgtagat aaaggtagag gttatgtttc
    15721 tgctgaagaa aataaaacag agaatgttcc aataggtgtt ttacctgtgg attcaatata
    15781 tactcctgtt gagaaggtta gttatcatgt ggaaaacaca agagttggtc aaaaaacaga
    15841 ttatgataaa ttagtattag aagtttggac taatggtagt ataaatcctc aagagggaat
    15901 atcattagct gcaaaggtat tagttgagca tttaaactta tttatagact taactgaaca
    15961 tgtaagtagt gttgaaatca tggtagaaaa agaagaagat caaaaagaaa aagttcttga
    16021 aatgactata gaagaattag atttatcagt tagatcatat aactgtttaa agagagcggg
    16081 aattaataca gttgaagaat tagctaataa atctgaggat gatatgatga aggttagaaa
    16141 cctaggtaag aagtcattag aagaggtaat acaaaaacta gaagaacttg gattgggatt
    16201 aaaaccaagt gaagaatagc gagcaaagga gggataagaa tggctaagta ccgtaaatta
    16261 ggacgtgaaa cagctcacag aaaccttatg ttaagaaact tagtaacttg cttactaaga
    16321 agtggaagaa tagaaactac agtgactaga gcgaaagaaa ctcgtagaat ggctgaaaag
    16381 atgataactc ttgctaagag aggagatctt catgctagaa gacaagtttt agcttatgtt
    16441 atggatgaaa cagtagttaa taacttattc acagatttag ctccaaaata tgctgagaga
    16501 aatggtggat acactagaat aataaaaata gggccaagaa aaggcgatgc tgctgaaatg
    16561 gcttttatag aattagtata gtaaggaaag gattaggctt gatggcttaa tctttttttt
    16621 atttttttga ctaaggtatg atataataat gtgtaaaatg atttttataa gttattgctt
    16681 actaaaagga gtatgagtta atggataata tagtaaaggt aaataatatt tcgtttgagt
    16741 atattacaga tgaagcaaaa cttaaagcaa tagataattt aagcctagat gttaaaaaag
    16801 gagaatttgt tgcgataata ggacataatg gttctggtaa gtctacttta tctaaaaact
    16861 taaatgctat tcttatgcct actgaaggaa atattcttat tgatgatatg gatactaaag
    16921 aagaagaaag attatgggat ataagacaaa ctgcaggcat ggtatttcaa aatccagata
    16981 atcaaatagt tgctactata gtagaagaag acgttgcatt tggacctgag aacttaggta
    17041 tagaaccaaa agaaataaga cgaattgtag aggagtcttt aaaaagtgta ggtatgtatg
    17101 atttaagaga tagacagcct catttgttat caggaggtca aaagcaaaga gttgcaatag
    17161 caggaattat tgctatgagg cctaaatgta taatatttga tgaagcaaca gcaatgttag
    17221 atccatctgg aagaaaagaa gttatgaaaa caataaaaag acttaataag gaagaaaata
    17281 taactgttat acatataacg cactttatgg aagaagcagt agaagctgat agagttgtag
    17341 ttatggaaaa aggaaagaaa atcttagagg gaactcctag agaagtattc agtaaaataa
    17401 agatgttaaa ggaaatcggt ttagatgtac cttgtatgac agagttatct agtttattaa
    17461 tagaagaagg tataaatatt agtagtgata tattaactgt ggatgagatg gtgatggaat
    17521 tatgtcaatt atagtaaaga atttaacaca tatatataat gaagggatgc cttttgcaag
    17581 taaggcactt gatgatgttt cttttgagat taaagataga gattttgttg gtcttatagg
    17641 tcatacaggc tcaggaaaat ctacattaat acaacattta aatggattac taaagccttc
    17701 ctcaggagaa atatttataa atgattttaa tataacagat aaaaacttga atttaactga
    17761 gattagaaaa agagtagggg ttgtatttca atatccagaa taccaattgt ttgaagaaac
    17821 gattgataaa gatatagctt ttggaccctc taatttaggc ttagaggagt ctgagataca
    17881 taacagggta aaagcatcaa tggaagctgt aggacttgat tatgagggat ttaaagacaa
    17941 atctcctttt gaattatctg ggggacaaaa gcgtagagta gcaattgctg gagttatagc
    18001 catgaaccca gaagttctca tattggatga accaactgct ggtcttgacc ctggtggaag
    18061 agatgaaatt tttaacttaa taaaagactt acatgaaaaa aagaatatga ctataatact
    18121 atcatcacat agtatggatg atatggcaaa gttagcaaaa actttaattg ttatgaatca
    18181 tggtagcgta gaatttatgg gaactcctag agaagtgttt aaatcaaatg ctagtaaatt
    18241 aaaagatata ggattagata ttccacaagt tttagaactt gcattgaaat tgagagaaaa
    18301 aggctttgac atcagtgagg atatattgac tttagaagaa gcaaaacagg aaatattaaa
    18361 agttgtgaga ggacgaggat tatgttaaaa gatataacta tagggcaata ctacccaaca
    18421 agttctgcta ttcataaatt agatccaagg ataaagcttg tagcgacaat tgtatttatg
    18481 gtatctatat ttgtagtaaa taaattttgg ccttatatag tagttttatt atgtttatta
    18541 gctatgataa aattggctaa tataccagtc aaatatattg ttaagggtgt taaacctctt
    18601 aaatggatta ttttgtttac atttcttata aatatttttt tcttaccagg tgatgaaata
    18661 tggtcatttg gatttttagc tattacaaaa cagggtctaa gacaagcaat ttttatggca
    18721 ataagattaa tatttttagt agttggtaca tcattgctta ctttaacaac ttcaccaata
    18781 gaattaactg atggaataga gagattgttg aatccattta agaaaattgg tttgccagtt
    18841 catgaacttg caatgatgat gacaattgct ttacgtttta ttcctacatt gttagatgag
    18901 acagacaaaa taatgaaggc tcagatgtct agaggtgcag attttgaaag taagaatctt
    18961 ataaatagag caaagaattt agtaccactt ttggtacctt tatttgtaag tgcatttaga
    19021 agagctgatg aattggctat ggccatggag gccagatgtt acagaggtgg acataatagg
    19081 actaagatga gagagtctgt tatatcaaga agggattata tggcatgtgt atttcaggtt
    19141 gtttatctgg gagcaattat agctactaga tttatagcaa tttaattaat aagttataga
    19201 tataatgagg taaagtatga gaaatataaa aattaaaatc caatacaatg gtaagaatta
    19261 ttgtggatgg caaaagcaac ctgattcttt agggatacaa ggtacaattg aaagggctat
    19321 atatgatata acaaaagaag aaaccagtct tatagggtct ggtagaactg attcaggagt
    19381 tcatgctatt ggtcagattg caaattttaa aattaattca ggtataagta tagaaagtat
    19441 accaatggca ctaaatgcca agttgccaaa agacatatca gttatagaag catgtgaagt
    19501 aaatgatgat tttcactcca gatacagtgc aaaaggtaaa acatataaat atttagtata
    19561 taatagcaag tttagaaatc caatacttag tgagatatca tatcaagtaa aatatgaact
    19621 tgattttgat aaaatgtgtt cagaagcaaa aagtttatta ggaacacatg attttaaagg
    19681 ttttatgagt tctggttcat ctgtaaaaga tacagttaga acgatatatg atattgatat
    19741 aagtaaaaaa gatgacttga ttacttttga gattagtggt aatggttttt tatataacat
    19801 ggtgaggatt attgttggaa cacttgttga tatgggtcgt ggcagaatta acgaaccatt
    19861 tttagatata atacaatcaa aaactaggtc aagatgtgga catacagctc cagcacaagg
    19921 attatttctc aaaaaagttc attattaact tgacacacca ggctacatgt attaaaatat
    19981 atgagaatgt gttaataagt ccacttgccc cggacttgac ataaacggta aaaaaatttt
    20041 atgaaaagta gttaaggagg gacagttatg aaaagttata tagctaagcc agctgatgta
    20101 caaagaaaat ggtacttagt tgatgctgaa ggaaaaacat taggtcgttt agcaacagaa
    20161 attgcgacag tattgagagg taaacataaa ccaacattta ctcctcacgt tgatggtgga
    20221 gattttgttg ttgtagtaaa tgcagaaaaa atagttttaa gtggtaagaa attagatcaa
    20281 aaatattaca gataccatac tggttatgta ggtggattaa aagaaatatc ttacagagat
    20341 atgatggata agaaaccaga agaagttata tcacatgctg taagcggtat gttacctaaa
    20401 aataaattaa gaagcagaat gatgactaga ttaagagtat ttgcaggtgc tgagcacact
    20461 catgcagctc aaaatccaga agttttaaac tttaaataag attaataggt gaaaggagga
    20521 gtttataatg gctaacgttc aatattatgg aactggaaga agaaaaagtt ctgttgctag
    20581 agtaagacta gttgcaggtg aaggaaatat attagtaaat ggaagagcat tagaaaatta
    20641 ttttaattat gagacattaa taagagatgt taaacaacca ttagttttaa ctggtaatga
    20701 aaataaatat gatgttatag taaaagttga aggtggagga ttcactggac aagctggagc
    20761 tataagacat ggaatatcta gagctttatt aaaagctgat ttagatttaa gacctgcttt
    20821 aaagaaagaa ggtttcttaa ctagagatgc aagaatgaag gaaagaaaga aatacggatt
    20881 aaaagcagca agaagagctc cacaattctc aaaaagataa ttacttattt ttttggaaaa
    20941 gcaaaatcct ttatttactg ttattcagaa agtattggta ttttaaaaat gttgggttaa
    21001 gttaattatt gatatataat aaaataataa gatatcttat taggatgttt ttcattaaga
    21061 agaacatcct ttttattttg acta
//
LOCUS       DBGJFU010000069        20522 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.069, whole genome shotgun sequence.
ACCESSION   DBGJFU010000069 DBGJFU010000000
VERSION     DBGJFU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.069"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     repeat_region   475..898
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=475..503
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1062..1235
                     /locus_tag="ACOIAS_002320"
     CDS             1062..1235
                     /locus_tag="ACOIAS_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGZ2702028.1"
                     /translation="MSSKMGRPKVDNPKNIDVKVRFDENTHKKLLDYCEKENLTRTEA
                     IRKGVDLLLEEDK"
     gene            1359..2186
                     /locus_tag="ACOIAS_002321"
     CDS             1359..2186
                     /locus_tag="ACOIAS_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HGZ2702029.1"
                     /translation="MNNELMNFENNELGIKIRTIKYEDGSIGINAEDTAVGFGWCQTQ
                     NKNGKQYVSIRWETINKYCKEFGFLNLLGKDEYIPESLFYLLGMKAKNEIAVKFQTWL
                     AVDVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSK
                     LLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPAQLAVCEIIKKIDL
                     EENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPAKIAGEKKNYHVVYKIE"
     gene            2249..2425
                     /locus_tag="ACOIAS_002322"
     CDS             2249..2425
                     /locus_tag="ACOIAS_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702030.1"
                     /translation="MCENLLNEYNLKTDEDVEYFVKFATLLYKLKQDNEEKFQEYAEI
                     LRGILREQQERENK"
     gene            complement(2838..3041)
                     /locus_tag="ACOIAS_002323"
     CDS             complement(2838..3041)
                     /locus_tag="ACOIAS_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702031.1"
                     /translation="MYFNIDKLLESKGRTRYWLAKEVGIAYPNMMKLANNETSSIKLD
                     LFEKLCLVLECTPNELVTLSSKE"
     gene            3285..4088
                     /locus_tag="ACOIAS_002324"
     CDS             3285..4088
                     /locus_tag="ACOIAS_002324"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGZ2702032.1"
                     /translation="MNNLVLINNQEVQVKEFNNQRVVTFKEIDRVHERVEGTAGRNFR
                     ENKKHFIKNEDYFYLEGKELSTIKQTTNFVGSNARELILLTETGYLMLVKSFTDDLAW
                     KVQRQLVNSYFRVKEEKKEMKALEKLETVNESIRLITPIFDDLNIDKSMKLLVTKTFF
                     ERAGIELPLEVEEKEHFYDTKQIAKKLGLYTMSNKPAFMAVKQIIRKLDIKEDEMKTV
                     LETNGNWTGTVTKYSNKVTEKVKVWLKENNNPISIKGDKKNYYILYKNN"
     repeat_region   4520..5471
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4520..4548
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            5601..6116
                     /locus_tag="ACOIAS_002325"
     CDS             5601..6116
                     /locus_tag="ACOIAS_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2702033.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREDE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     gene            6181..8574
                     /locus_tag="ACOIAS_002326"
     CDS             6181..8574
                     /locus_tag="ACOIAS_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGZ2702034.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIDSITKVADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMAITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVSNVTLGAILIINGLGWVTNATLNVANVFAQNWSIIAPIIYG
                     VIAAIAIYKGVLLASTIATTVASFVNSLYAVAAYKSCAALAAQEFAIFGKISAQTMEA
                     LVTAQATAAQWGFNAALLSCPIFWIIAGIIAFVVVVFVAVAAVNKFSGTSLTVLGAIV
                     GAVFAAVAFIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DAVNNAGNALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNKWVGDAPEKIKLDRMGYKDIGAEFQKGNALGTKWQNAITDKFKDTFDINK
                     MLEDAKKKLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMIVSAEGIV"
     gene            8590..9279
                     /locus_tag="ACOIAS_002327"
     CDS             8590..9279
                     /locus_tag="ACOIAS_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGZ2702035.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKIVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGAFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDAENGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKKVPKY
                     TIYKGQVLRLV"
     gene            9272..11164
                     /locus_tag="ACOIAS_002328"
     CDS             9272..11164
                     /locus_tag="ACOIAS_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGZ2702036.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPAVIDGITWEIERRGSPSK
                     LEFTIAMDDILEFCEGNSVRIYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDVLLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            11178..11438
                     /locus_tag="ACOIAS_002329"
     CDS             11178..11438
                     /locus_tag="ACOIAS_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HGZ2702037.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            11443..11862
                     /locus_tag="ACOIAS_002330"
     CDS             11443..11862
                     /locus_tag="ACOIAS_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HGZ2702038.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFENIKGKVQCKFSVHTKYGNIKAEKVVSV"
     gene            11863..12912
                     /locus_tag="ACOIAS_002331"
     CDS             11863..12912
                     /locus_tag="ACOIAS_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGZ2702039.1"
                     /translation="MFELMTFENIIKRMLDSVPNTLDKREGSIIYNALAPVAVELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            12905..13522
                     /locus_tag="ACOIAS_002332"
     CDS             12905..13522
                     /locus_tag="ACOIAS_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HGZ2702040.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            13534..14526
                     /locus_tag="ACOIAS_002333"
     CDS             13534..14526
                     /locus_tag="ACOIAS_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGZ2702041.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTQRIVVNIIDATTKENIVPNFKIVDDNSIEIRSEVKVELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDRLVNIEEKVNGGLSNIATSVNELITYC"
     gene            14543..16255
                     /locus_tag="ACOIAS_002334"
     CDS             14543..16255
                     /locus_tag="ACOIAS_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HGZ2702042.1"
                     /translation="MQTEWNFNYANYVQNVSLPPGRYKLECWGACGGAVDTSDWTDCA
                     KGGYSKGEIVFKKRTNLQICVGQSGYEKVSEGSSLTRSGFNGAGAAGKVTTGSFAYSK
                     YGGGATDIRLYHPSATWGNTESLLSRILVAGGGGGMKNNFASARSIGHGGGYVGVNGV
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGNIGIGGGGGWYGGAGSYSNECGGG
                     GSGYALNKDSYKVPGYIPTPEYYLENIVMTTGGNTTKADGYAKITLLQALPFLTVSSY
                     NSTQATFKADHTDPALLTKIEWFIDDVLKETITTNLTEEKTINYTLEDNALHTLKIVV
                     TDSNNATAEKVLSISKNIMPLPENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIE
                     ASLNNTLVELSEKIKTSFDSSDTSVQDLQNQVTQKNNTITQLETELSKRKRFITGTYT
                     FTKTDAENFNLSIYDKEGTSKTLTIPVNMGFSPSLIVLSGVTFSTTSKSYVYFDNVCN
                     SNFYNFGYNSDSTHSNPKAVGILNVSNVGYSSLVLTLYKLSMSEAVGIWAKEGATLTY
                     KIYI"
     gene            16276..16623
                     /locus_tag="ACOIAS_002335"
     CDS             16276..16623
                     /locus_tag="ACOIAS_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702043.1"
                     /translation="MNRNNRIIYDQTGNIWLQTGEATGDIQEWSKITELNFLDVEFGS
                     IDYSKQYIESINPVTKEPIIKDIEVILTDEQKRLQALEKELSMLKEENKNRDSEIVNT
                     AFEVENIKLNNNL"
     gene            16635..16805
                     /locus_tag="ACOIAS_002336"
     CDS             16635..16805
                     /locus_tag="ACOIAS_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702044.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKIDVFYAVNRITEEQYLELTSL
                     LNKEETPAEPIK"
     gene            16836..17402
                     /locus_tag="ACOIAS_002337"
     CDS             16836..17402
                     /locus_tag="ACOIAS_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702045.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            17433..17723
                     /locus_tag="ACOIAS_002338"
     CDS             17433..17723
                     /locus_tag="ACOIAS_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702046.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            17727..17984
                     /locus_tag="ACOIAS_002339"
     CDS             17727..17984
                     /locus_tag="ACOIAS_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGZ2702047.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGVNQTYKQFKDGEK"
     gene            18245..19108
                     /locus_tag="ACOIAS_002340"
                     /pseudo
     CDS             18245..19108
                     /locus_tag="ACOIAS_002340"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
     gene            19187..19999
                     /locus_tag="ACOIAS_002341"
     CDS             19187..19999
                     /locus_tag="ACOIAS_002341"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2702048.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            20093..>20522
                     /locus_tag="ACOIAS_002342"
     CDS             20093..>20522
                     /locus_tag="ACOIAS_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2702049.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDL"
BASE COUNT         8111 a         2241 c         3829 g         6341 t
ORIGIN      
        1 ataataatcg aaagtgcgaa gggtgattat tttcatatta aacgccaaat tccaaataag
       61 gaaggaggtg aaattatatg ataggttttt tattaagcat actagctggt gttatatcag
      121 cttatattta tgacaaaata aaaaatcacc cagacgccaa taagggtgat ttaaaaaaat
      181 aattctttaa atcaattttg atggaaatag ctactcttgt ataaagtaaa ttatttcctt
      241 gcttttatta taccacaaat tggtacagat attcaaaaat aatattttta tgatataata
      301 aaaatgtaga gattttgcag tgagcaatat ttgcgataaa ttgaagttta acaattggaa
      361 tacaaggtat tgagggtgtg tgataaatgt tatcaattgc actactcatg gttcactgca
      421 aatttgagag agatgcgtat gtgtaggtat tggaaatgcc aagtttattt tggggtttta
      481 gattaactat atggaatgta aattaacctt tttattgttg ttgttattct tattgttatt
      541 cgttttagat taactatatg gaatgtaaat gttgtaagaa gtatcattct attttttaat
      601 ctttctgttt tagattaact atatggaatg taaatagtac atataatgag tctttaacat
      661 cagttatgaa aggttttaga ttaactatat ggaatgtaaa tgattgtact ttagcgtctg
      721 cactagcttt gtctatcgtt ttagattaac tatatggaat gtaaattatt ttacagatga
      781 acaattacag ttacttcttg aatgttttat attaactaag tggtatgtaa ataatggaag
      841 caagataatt tttaaaggta tggataattg ttttatatta actatgtgga ttcaaaatta
      901 aatagaaaaa agaaagcact tacttaaata gtaggtgctt ttgttttgct caaaattggt
      961 cggttgagta aaataattag aaaaaattgg gataagttat tgactttttg gaacacaaat
     1021 aatataatta tattatggaa cacaaaaagt gaggtgaaac aatgagttct aaaatgggaa
     1081 gaccaaaagt tgataatcct aaaaatattg atgtcaaagt aagatttgat gaaaatactc
     1141 ataaaaaatt attagattat tgtgagaaag aaaatcttac tagaacagaa gcaataagaa
     1201 aaggagtaga cttacttttg gaggaagata agtaaaaaag tagcccaacg ccgaccaaag
     1261 catactggac tacttaacct agagttatct ctatatgaaa tattctatca tgtaaagata
     1321 actctttcaa gataataaat cgaaagggtg atttttgtat gaataatgaa ctgatgaatt
     1381 ttgaaaataa tgaattagga ataaaaataa gaactattaa atatgaagat ggaagtatag
     1441 gaattaatgc agaggataca gctgtagggt ttggatggtg tcagacacag aataaaaatg
     1501 gtaagcaata tgtatcaatt agatgggaaa ctattaataa atactgtaaa gaatttggtt
     1561 tcctcaactt gttggggaaa gatgagtata tacctgaatc attattttat cttttaggta
     1621 tgaaagctaa aaatgaaata gcagtaaaat ttcaaacatg gttagcagta gatgtactac
     1681 catcaataag acaaactggt gcatacataa ctaacaatgc taatcccgaa aaactaagag
     1741 aaaaagcaag tgagattgaa aagttacaat tagcctataa cagcacatct atgttaaaag
     1801 aattgctaga tggtgcagga tttgacaata aatccaaact attaacagct aaaacattat
     1861 ataaaaaggc aggcattgat ttacctatag aaattgaaga agaggaatct ttctttgaca
     1921 caaaacaaat agcatctaaa ctgaaaatat attctaagag taataaacca gcacagttgg
     1981 ctgtttgtga aattattaaa aagattgatt tagaagaaaa cgaagtcaaa ggcgtttggg
     2041 aaactaatgg ttcttggact ggtactgtaa ataaatatac aaagagtgta atagataagg
     2101 ttagaaattg gatagaggaa aataatagac ctgctaagat tgcaggtgaa aagaagaatt
     2161 atcatgtggt ttataagatt gagtaaattt atcagttgta ttaaataatt tagtttagtt
     2221 tattttagtt ttgaggggga ttaatacaat gtgtgagaat ttacttaatg aatataattt
     2281 aaaaactgat gaagatgtag aatactttgt aaagtttgct acattattat ataaattaaa
     2341 acaggacaat gaagaaaaat ttcaagagta tgcagagata ttgagaggta ttcttaggga
     2401 acaacaagag agagaaaata agtaaaataa tatagataaa gcacttgaat attacatagt
     2461 ttcaagtgct ttgtttggta taaaatggta taatagagat aagagttata ttaactatgt
     2521 ggtatgtaaa gaatgtataa ggcatcttct caaattctgc gttggtcatt ttatattaac
     2581 tatgtggtat gtaaagtgcg ttaactgcaa taatgctgaa ctcatttctt gattttatat
     2641 taactaagtg gtatgtaaat gttaattcct tcaaccttct aatagaagat gcttttaggt
     2701 ttatattaac taagtggtat gtaaatgttt caacatttga catgtgctgt ggagtaatgt
     2761 ttatgtttta tattaactat gtggattcaa aattaaatag aaaaaagaag cacttactta
     2821 aatagtaggt gcttttgtta ttctttgcta gataaagtaa caagttcatt aggtgtacat
     2881 tcaagaacaa gacaaagctt ttcgaataaa tctaatttta tagaacttgt ttcattgtta
     2941 gctaatttca tcatgttagg ataagctata cctacttctt tagctaacca atatctagtt
     3001 cgacctttgg attcaagtaa tttatcaatg ttaaaataca ttttttcacc tcttaagtat
     3061 attatacaac aattatataa tgtttgcaat atataatatg caaacaatat aattatataa
     3121 tgttgacatt atataatgtg tgcgatataa tatacttaag gaataaaaaa taagagccac
     3181 tcgcccctac caagtttgag taactcttat tgacatatac tacatataca ttaattatag
     3241 tatatgtcat tccttaaaaa aaatcaatta aggagtgtgt ttttatgaac aatttagtac
     3301 taattaacaa tcaagaagtg caagttaagg aatttaataa tcaaagagta gttacattta
     3361 aagaaattga cagagtacat gaaagagtag aaggaacagc aggtagaaat tttagagaaa
     3421 ataagaagca ctttataaaa aatgaggact atttttattt agaaggaaaa gaattatcaa
     3481 caattaaaca gacaacgaat ttcgttggca gtaatgcaag agaattaatc ttattaactg
     3541 aaacaggtta tctaatgtta gtaaaatctt ttacagatga tttagcttgg aaagtacaaa
     3601 gacaattggt aaatagttat tttagagtta aagaagaaaa gaaagaaatg aaagcattag
     3661 aaaaattaga aacagttaat gagtcaatta ggttaataac tccaattttt gatgatttaa
     3721 acattgataa aagtatgaaa cttcttgtaa ctaagacttt ttttgaaaga gcaggtattg
     3781 aattgcctct tgaagttgaa gaaaaagaac atttttatga tacaaagcag attgcaaaaa
     3841 aattaggact ttacacaatg tctaataaac cagcatttat ggcagttaag caaattataa
     3901 gaaagcttga tattaaagaa gatgaaatga aaacagtatt agaaacgaat ggcaattgga
     3961 ctggtactgt aacaaaatat tctaataagg ttacagaaaa ggtcaaagtg tggttgaaag
     4021 aaaataataa tcctatctca atcaaaggag acaagaaaaa ctactatata ttgtataaaa
     4081 ataattagat tttagttttt gggggggtaa tacaatgtat gagaatttac ttgatagtat
     4141 agatattgaa aagagaaaag aagaatttag aattaaactt ttgaaaataa gagaaacaga
     4201 tatagatata tatatataat aagatagaaa gaatagtata taaactttct gagaaaaaat
     4261 tagagaaaaa taattaaata aatagataaa gcacttggat attatgttgt ttcaagtgct
     4321 ttatttggta taaaatggta taataaaaat ataggaattt tgcagtgttc gatttttcta
     4381 cttaagtata gtttaacaat tggaatacaa ggcattgaga aggtttgata agtgttatca
     4441 attgcactat tacccgctca ctgcaaattt gagaaagtta tatgtgtgta agtcttggaa
     4501 atacacaatt tattttgggg ttttatatta actatgtggt atgtaaatct ttttaaaaaa
     4561 gcacgtcaat tacaatacta aaattgtttt atattaacta tgtggtatgt aaatttgaaa
     4621 actgcatgtt ctttaacaaa agcatctaat tgttttatat taactatgtg gtatgtaaat
     4681 gtattactcc caattatgaa cataggatat agtctttgtt ttatattaac tatgtggtat
     4741 gtaaattata gtttgccata tgttcctggt tcgtatgatt ggggttttat attaactatg
     4801 tggtatgtaa ataaatttaa tattactgga aaaggagaaa tggatatcgg ttttatatta
     4861 actatgtggt atgtaaattt tgttataaga atatcgccac cttccttttc tggtgtttta
     4921 tattaactat gtggtatgta aatttttctg catcttctac tatgttatat tcttctaatg
     4981 gttttatatt aactatgtgg tatgtaaatg cacatttaac ttcaagtctt agagtattat
     5041 ctgcaagttt tatattaact atgtggtatg taaatttcgt tcaactttta tcttagcttc
     5101 tcctgctact tgttttatat taactatgtg gtatgtaaat gtactagaaa atgcactacc
     5161 tgcattcatt cctatacggt tttatattaa ctatgtggta tgtaaatgtg ctatttccaa
     5221 cttttttttc aagttctgaa tactgtttta tattaactat gtggtatgta aattagttag
     5281 tcccatatcg ttatggtact gcattaacgc gttttatatt aactaagtgg tatgtaaata
     5341 gtgtaaaagg cattttttca aattgtgcat tggtttggtt tatattaact aagtggtatg
     5401 taaaggctaa tgtttgagta tttaaaagca tctgagcaaa caattttata ttaactatgt
     5461 ggacttaaaa ttaaaaataa ttcaaaaaca cttacaaatg agtaagtgtt tttttaatga
     5521 aaggaggtga taataatgta aaaattttac gtatatagta taatagtgct ataaaataag
     5581 tattatgtga ggtgattgtt gtgttttgtt caaattgtgg ttatgagata actggtgcag
     5641 gcaaattttg ctcaaattgc ggaacagcta cattagcaga taaagttaac aatgatgatt
     5701 tatttataaa tgttcatgga aaagaattaa atctgactaa tatttataaa gaaactaaag
     5761 gagataaaat cttagcaatt gatattgcaa tgaagttact agggctggac ataaaagagt
     5821 gtaaaaatat tatatatcca gcttttaaag aattaagtga aaaaataaat atcgaagagg
     5881 aaaaagagat attgagggaa gatgagtata aacaaactaa tgtacttgaa gatgatgttg
     5941 ctcgttgccc taggtgtggc tctgtttcat tgtctgctca taagaaaggt tttggcatag
     6001 gaaaagctgt agcaggggct actatagcag gaggcattgg tttagtagct ggaaatttag
     6061 gagcaaagaa agttagagtt acatgtttga gctgtggtaa acagttttgg gcataaataa
     6121 taaacactta ctaatgtagg tgttttttta tatggaaatt tatgaaagga gagtgaggaa
     6181 atggctacaa tacaaacttc aataaagatt ttcgacggaa tgacaccagc atttcgtaat
     6241 atgactaatt ctattaatac aacaattaat agtttagaga gattgcaagg cagattgaat
     6301 aatccactca atgcaggtaa tatacaagct tctcaacaaa gtttgaataa catagaaagt
     6361 attctcacaa ggatagaaca gaaaattgga agaaatacaa atgaacagga aaactttaat
     6421 aataaaataa gacaaggtag tgaagcaggt tctctattag tgtctaaatt aaaaagtatt
     6481 gctgggatat acattggaat aaaaggaata gatagtatta caaaagtagc agatacaatt
     6541 gcaagtacaa aagcacgttt aaatctaatg aatgatggct tacagacaac agaccagctt
     6601 aataaaatga tttatttgtc agcccaaagt gcaagagcta gttatgcaga tacagcagca
     6661 cagattgcta aacttggaat acttgcagga gatgcttttg gaagttcagc agaggtgata
     6721 aagtttacag aacttatgaa taaagctttt gtaattggag gaacatcagc aaatgaagct
     6781 agtgcagcaa tgtatcaatt aacacaagcc atgggtgcag gaaaacttca aggtgatgag
     6841 ttccgttcca taatggaaaa tgcaccttta ttagccacta aaatagctga tgcaatggga
     6901 aaaactaaag accaattgaa ggaattatca agtagcggag caataacagc agatgttata
     6961 agaaatgcac tgtttaaagc ttctgatgag atagaaaaga aatttgcaag tatggcaatc
     7021 actttttctc aagctctcac aatgatgaaa aatgatgctt atatgatatt ttctgagact
     7081 ctcagtaaga taagtggagc tttgcaaagt gtacgtttta gtgagattgt tgtatctatc
     7141 cggaatgtta tgattgcaat atcttcaaac atttatgata cattaaatat tataaaaaat
     7201 atattaaata gtgatttttt ttcagatttt gttagcaatg ttacattagg agctatacta
     7261 attatcaatg gactgggatg ggttactaat gcaacactaa atgttgctaa tgtctttgct
     7321 caaaattggt cgattattgc tcctattatt tatggagtga tagcagcaat tgcgatatat
     7381 aaaggagtat tacttgcaag tacaattgcc actacggtag catcttttgt aaattcattg
     7441 tatgcagtag cagcgtataa atcttgtgca gctttagcag cacaagaatt tgcgatattt
     7501 ggaaaaatat ctgcacaaac gatggaagct ttggtaacag cacaagcaac agcagcacaa
     7561 tggggtttta atgcagcatt attatcctgt ccaatttttt ggataatagc aggtattata
     7621 gcatttgtag tagtagtttt tgttgcagta gcagcagtaa acaaattctc aggaacaagt
     7681 ctgactgtac taggagcaat tgtaggtgca gtatttgcag cagtagcatt tatacaaaac
     7741 ataatgatat ggctatttaa tagatgcgta gatgtaaatg aagggattgc aaatggatgg
     7801 aatcaatgtg tatatctaat gaaacaagct attgcaaaag gtgtaatctt tataattgag
     7861 aaaatggcat cattaaatga tgctgtaaat aatgctggaa atgcacttgg gaaagctttc
     7921 atagatgggg caaatatcgc aatacgaggt gtaaacaaat taattgacct aataaataaa
     7981 ataccaggga taaatattgg taaagttgga gaagcaacat ttacgcctgt caaggcagat
     8041 aatagttaca tcaaacaaca gattgacagt ttaaataaat gggtaggaga tgcaccagaa
     8101 aaaataaaat tggatagaat gggatataaa gatattggag cagaatttca aaaaggaaat
     8161 gcacttggaa ctaaatggca aaatgctata actgataaat ttaaagatac ttttgacatt
     8221 aataagatgc tagaagatgc aaagaaaaaa ttaggattag acgatttgtg gaataaacaa
     8281 aatcctttaa acaaccttgg tggatttggt ggagatttag gaaaaaatgt aaaagacaca
     8341 gcaggaaaca cagccaaaat ggctaaaaca atggataaaa gtcaagaaga ccttaaatac
     8401 ttaagagaca ttgcagaaca agaaacaata aacagattca caggagtaaa cataaaaatt
     8461 gatatgaaca atactaacaa tataagtaaa gacacggatg tggatggtat agtaaatgtt
     8521 ttaacagaaa aactgaatga tgctatgatt gtatcagcag aaggaatagt ttagaaagga
     8581 gggatataaa tggcttatga cttttattta gatggagtac aattaccaat acctccgcca
     8641 aagttagaga ttaaagttac aaataaaaac aagatagttg atttgataaa tactggagaa
     8701 gtaaacatac taaaaaaaga aggattatct gaaataagtt ttgaagcaga atttacacat
     8761 aataagttgc cattctatcg tggagctttt agggatgttc aattcttttt aagtaaactg
     8821 gaactattaa aaactgattg taagccattt caatttattg tatcgaggga attaggtaat
     8881 aaagtactat ttaacactaa tataaaagta tctcttgaag agtatgctat ttcagaagat
     8941 gcagaaaatg gttcagatac aaaagttgca ataaagttaa agcaatatag agattactca
     9001 actaaaaagt tagttcttgc ccctcctaaa aatgagactg gtagacctaa tgtaaagata
     9061 gagccaaaac gagttgattc agtcaatgcc acaaacacta aaacatatac agtaaaagca
     9121 ggggatagcc tttggtcaat ttgtcagaag caacttggta atggttcgtt atacaagaaa
     9181 gtatatgagt taaataaaac aatgatggat aaagctaaca agggtaaaaa agtacctaaa
     9241 tataccatct ataaagggca ggtgttgagg cttgtctgat gatttagttc tggcgaatga
     9301 tagagatata aggctagtta ttgcacattg ggaagatttc tacgaacctg ctgtcattga
     9361 tggtatcaca tgggagatag aaagaagagg ttcaccttca aagttagagt ttactatagc
     9421 aatggatgat atattagagt tttgcgaagg taattctgta aggatatatt ataagggaat
     9481 tggcatattt tatggatata tatttcagaa gaaaagagat aaagaaaatc acattaaaat
     9541 agttgcttat gaccagttga gatattttaa aaataaagat acttatgtat atagtaataa
     9601 aacagcaagt gaacttgtaa agatgttggc taaagatttt aatttaaaat acaatgtcat
     9661 agaagataca aaatataaaa tatcgagagt tgaagaaaat aaaacactct ttgatatggt
     9721 cttaactgca ctagatgata ctctaagaga gaaaaaagaa atgtatgtgt tatatgatga
     9781 ttttggaaga ataacattaa agaatgttgc atcaatgaaa ttagatactg ttatgaacaa
     9841 tgatgtaata gaagactttg actataattc atcaatagat agtgatactt acacaaagat
     9901 taaacttgtg agagataatg aagaaacagg aaaaagagat gtgtatattg ctcaagactc
     9961 aacgcatatg aggagttggg gaatacttca aatgtttgat acagtagaca aaaacatgag
    10021 tgaagcagag ataaaacaaa agtgtgatat acttctaaaa ctatataata agaaaactaa
    10081 gtcattaagt ttaaaaaatg tacttggaga tattagagtg agagcaggtt gtttagtacc
    10141 tgttttttta aatctaggag atattaaact tcaaaattat atgctagttg agaaagtaaa
    10201 acatactttt gaaaataaca gtcattttat ggatttgact cttgttgatg gagatgaatt
    10261 tgcttcttat tcttcaagct catattcaag tggaaatact aacaataagg atgaaaagaa
    10321 aaatggtcct gcacaaagta ttacgaaaaa aaatacaggt aaaaaagttc ctgctatatt
    10381 tactgcatat tatccaggga acaatgcaat ggaaggcgga aaaacagatt gcaatggaaa
    10441 gccacttgat gtaaaatcaa gaactgttgc tggtccaatg aatcgagaag gagttaagaa
    10501 aacttggtat actgatgatt ttctaaagaa acatccagtt tttgaatatg gagataaagt
    10561 aaaaattata cttcctggta ctgcctatga caacaaagta tatacagtta aagataatgg
    10621 aggaagaata tatgttgaaa caaacggaac atatcatata gatgtactat tagctaatgc
    10681 tagtgaatgt aaaaaatttg gtagaaagaa tggctatata attataggtg gagatgaaga
    10741 acaaacatat caagttgaag gtaataacca aagtagtaca aataataact ctaaagaaga
    10801 taaattaatt agtatagcaa aaagtaaact gggttgtaat tatgtgtatg gagcagaagg
    10861 tcctaataat tttgattgca gtgggtttac tcaatggtgt tataaacaaa taggtataaa
    10921 aattcctcgt actgcttctg cacaaagtaa agcaggaaaa gcagtagatt taaaagatag
    10981 aagcaagtgg aaagcaggag acttattatg tagaattgga ggaggaagta gtaatcatgt
    11041 tgtaatgtat attggaaaca atcaaataat tcattcacca caaacaggag atgtggtaaa
    11101 aatagagtct gttaattctt atagaaaagg aaaagcatac acacatgtga gaagatttat
    11161 ataagtgagg tggcaatatg agccaagatt tattacagat aataaaaaaa gctgcaatgg
    11221 atgcagtaga aacaagcaac ccaatgaggg ttgtatttgg aacaatagaa agtattagtc
    11281 ctctaagagt taagatagaa caaaaactat ctattggtga aatttttcta atacaaacag
    11341 atacatttaa aagatataca gataaaaaaa taggagataa attagtctta attcgtatgc
    11401 aaggagggca acaatacttg attttagata ggatgtgata aagtgttacc aagtgataat
    11461 ttagattatg atattgaaga tgtatcaata attaattttg atgtaagaca agagccaagt
    11521 aagacattta aacttcatat agaaaagtct aagattgatg gtatttgtga tgatgtagaa
    11581 gcattaaaac agaccatctt tttgatttta aacacagaga gataccaaca tctaatatat
    11641 agttggaatt atggagtcga gttgaacgac cttattggag agcctatatc ctttgtaata
    11701 ccagagttag agagaagaat caaagaagca ctaattcaag atgatagggt tgaaaatgta
    11761 gataattttg agtttgaaaa tataaagggt aaagtacaat gtaaattttc agttcataca
    11821 aaatatggaa atataaaagc agagaaggtg gtgagtgtat aattgtttga gttaatgaca
    11881 tttgaaaata taattaaaag aatgttagat agtgtaccta acacacttga taagagagaa
    11941 ggttctataa tatacaatgc cttggcacca gttgcagtgg agcttacaga aacatatatt
    12001 gctatggatg aattactaga ccaaacattc gtagatactg ctagttatta ctatttagag
    12061 aagagatgta aagagcgagg aattacacct ttaccagcca ctaatacaat tgctaaagga
    12121 gtttttaaca tagatattcc acttgattct aggtttaatc taggagaata caattatatt
    12181 gcaattgaga gaatatctga aaaaacatat aaaatgaaat gtgaaactgc tggacctata
    12241 tttgagttag gaaaactaat acctattgaa tatatagatg gtcttgaaac tgctgaacta
    12301 actgaaatct tgataaatgg agaggatgaa gagtcagaag atagtttaag acaaagatat
    12361 tatgatagcc taaattcaca gagctttggt gggaatatac aaaactataa agatgaagtt
    12421 aacaaaatac aagatgttgg aggagttaag gtttatcctg tgtgggacgg tggaggaact
    12481 gttaagttag taataattaa ctctaatttc aaagtaccat cagaggattt agttaattta
    12541 gtgcaagaag aaattgaccc aattggacat caaggacaag gcttaggatt agcaccaata
    12601 gggcataaag ttactgttac aggtgttgta agtacaacta taaatatatc agcagagata
    12661 acatacaaaa atggctacac ttgggagaat ataaaatcaa ttgcagaaga agcaatagac
    12721 gactatttaa atgaacttaa catgagttgg gaagatgaag aaaacttaat agtccgtata
    12781 tctcaaattg aaactagatt acttagtata gatggagtgt tagatattac aaacacaatg
    12841 ataaatgatg ttaaatctaa tctaacaata gatagtaaca gtatagtagt gagaggtgag
    12901 gtagttggat aaagagatta atctaataaa ttacttacca caaattctac aagataaaga
    12961 agaatatata aaagtattta atgtagaaaa caaagaaata aaaacactac atgaaaaatt
    13021 aaatgaccta tcaagtgacc agtttttaga ggatttaact ccaagtggta taaaaagatg
    13081 ggaaaagata atgtctataa ctcctaaaag taatgagagt ttagaagata gaaggtttag
    13141 gatttttagt aaatatataa gtaaactacc ttactcagag agatttttaa ggaactggct
    13201 agatagtata gttggagaag gcaattatga attaactatt aataatgcta cttataatat
    13261 acatcttgaa agtgatgcta gaaatcaaga ttggtttgag gaggttcatt cttttgtaag
    13321 tagtattaaa ccttgcaaca tgactttaga ttacactaga gtgcttgtaa gtaaagacaa
    13381 ttatatgaat tttggtataa caaccctaat aggtcaagaa ataactatat acccttggag
    13441 tccaccagat atagaaactt atggagaaat tgatgtatta actggcaatg gagttggata
    13501 ccaagagata acaatatttt aggaggtgat atattggcta tagataaaag ttattacact
    13561 ataattacag atgtagggaa agcaaagata gcaaatgcaa gtgtcacagg taataaagtg
    13621 ggatttgtaa aaattcaact tggtgatgga ggagggagtg aatatactcc aactgagagt
    13681 cagacagctc tcaaaaatgt ggtatgggaa ggcaatatcg gaaatacaac tacagatgaa
    13741 actgcaccaa attgtataat attagagagt ttaataccat caagtgtagg cgggtttatg
    13801 ataagagaaa taggatattt agatgatgaa aataatttaa ttgccatttc taaatacaaa
    13861 gagtgttata aaccttctat agaacaaggt gcagtggtag acatgaaggt taaaactgtg
    13921 cttattgtat ctaatgtaaa taatatagaa cttaaaattg acccaacaat aatctttgca
    13981 acactcaaag atatacaaga cttagaaact aaaataggta ctgttaatac taaaattgat
    14041 acaactaaaa cagaattaac aagcaacata gaaactgcta aaacagagtt aaatactaaa
    14101 atcgaccaat taatcgcagg tggctcaaat gttgcataca ctcaaagggt cgcaattgat
    14161 gattgggttg aggatgcaga aagtggattc aaagcaactg taacacatag tttattaaca
    14221 cagagaatag ttgtaaatat tatagatgct actacaaaag aaaatatagt tccaaatttt
    14281 aaaatagttg atgataattc aattgagatt agaagtgaag taaaagttga gttaaatgtc
    14341 tatgtgataa atggaaatgc agaaactcat tttataaatg caactgtaga tgataacaga
    14401 gtatctgaaa tgactactta ttcatctaag aaaatagagg acagattggt taatatagaa
    14461 gaaaaggtaa atggtggttt atctaatatt gcaacaagtg taaatgagtt gataacttat
    14521 tgttagagag gagagtgaga aaatgcagac tgaatggaat tttaattatg ctaattatgt
    14581 acaaaatgtt tcattgccac ctgggcgata taaattagaa tgttggggtg cttgtggtgg
    14641 tgctgtcgat acaagcgatt ggactgattg tgcaaaaggt ggttattcaa aaggtgagat
    14701 tgtatttaaa aaaagaacta atctacaaat ttgtgtcggt caatccggtt atgagaaagt
    14761 ttctgaaggt tcaagcctta ctagaagtgg ttttaacggt gcaggcgctg ctggcaaagt
    14821 tactactggt agctttgctt attctaaata cggtggtgga gcaactgata taagacttta
    14881 tcatcctagt gcaacttggg gtaacaccga aagtttgctt tcacgcatac ttgttgcagg
    14941 cggtggagga ggtatgaaaa ataattttgc ttctgctcgt tctattggtc atggtggtgg
    15001 ttatgtaggt gttaatggag ttggtcgtga cagagatttt tgtggcggtg gttctcaata
    15061 ccaaggtgga acaagttacg acacagaaga ataccatggt tcattaggaa aaggaggtta
    15121 tggtaacata ggaataggtg gtggaggggg ttggtacggt ggtgctggtt cttattctaa
    15181 tgaatgtgga ggtggtggaa gtggttacgc actaaataaa gatagttata aggtacccgg
    15241 atatatacca acacctgaat attatcttga aaatatagtc atgactactg gaggtaatac
    15301 tactaaagca gatggttatg ctaaaataac attactacaa gcattgccat ttttaacagt
    15361 atcttcttat aattctacac aggcaacatt taaagccgac cacacagacc ctgcattact
    15421 tacaaagata gaatggttta tagatgatgt actaaaagaa actataacaa caaatttaac
    15481 agaagagaaa acaattaatt atacattaga agataatgca ttacacacac ttaaaatagt
    15541 tgttacagac agtaataatg ctacagcaga aaaagtgtta agtataagta agaatataat
    15601 gccactgccc gaaaatgtaa atttaaatga tatatcaaca aaattagttg aggttaatgc
    15661 aggatttaaa gttgggaaaa caagtattat aaacacttta gcacttaaaa atatagaagc
    15721 aagtttaaat aatacacttg tggagttatc agagaaaata aaaacaagtt ttgatagttc
    15781 agacactagt gtgcaagatt tacaaaatca agtaacacaa aaaaacaata ctataactca
    15841 attagaaaca gagttaagta aaagaaaaag atttataaca ggaacttata cattcacaaa
    15901 aacggatgca gagaatttta acttaagtat atatgacaaa gaaggaactt caaaaactct
    15961 tactatacca gttaacatgg gcttctctcc tagtttaatt gtacttagtg gagtaacttt
    16021 tagtacaaca agtaaatctt atgtttattt tgataatgtt tgtaattcaa atttttataa
    16081 ttttggctat aattctgatt ctactcatag taacccgaaa gctgttggta ttcttaatgt
    16141 atctaatgtt gggtattctt cattagtgct tacattatat aaattatcaa tgtcagaagc
    16201 agttggaata tgggcaaaag agggagctac attaacttac aaaatatata tttaataaaa
    16261 aaatgaggtg ataatatgaa cagaaataat agaataattt acgaccaaac aggcaatata
    16321 tggcttcaaa ctggtgaagc aacaggagat atacaagagt ggtcaaaaat aactgaatta
    16381 aattttttgg atgttgaatt tggtagtata gactatagta aacaatatat agagtctata
    16441 aatccagtta caaaagaacc aatcatcaaa gacatagaag tcattttaac agatgaacaa
    16501 aagagattac aagcattaga aaaagaatta agcatgttaa aagaagaaaa taaaaataga
    16561 gatagtgaga tagtaaacac agcctttgag gtagaaaata taaaattaaa taacaattta
    16621 taggaggaat taacatgtat aacttattaa aacttatgat agaacaaaag aactatagca
    16681 ctaaagagga tttgcaacac aaaattgatg tattctatgc agtaaataga attacagaag
    16741 aacaatattt agaacttaca agtttattaa ataaagaaga aacaccagca gaaccaataa
    16801 aataggttct ttttttattg aaagaaggtg actaaatgac ttttaaagag ttagttaata
    16861 aagttagaaa tcttgtatta gaagcaaaga atgtaactat agaagataca gaaagtaaat
    16921 ttacaagtga aaatgtagaa ggagcattga aagaatgtat agatagagca gatgaggctt
    16981 ttcaagaagc cgatagtgga aaaacacttt tatcaactgc tatcggctct cctgctacat
    17041 cagaacaaac atttcaagat tatgcgaact atattacagg atttaagagc aatataagca
    17101 atttagaaac tcaattgaaa agcaaatatt ctattagata tggtcctatt gatggatatg
    17161 atggtaatcc tttttctgct aattttggca agagtgcaag ttaccttatt gtctatgttt
    17221 actttagaag aagtgtatat tattataatc ccagtggtag ttctttagga agtaatacag
    17281 gaggttctga acgtgcatgg attactatag acagcaataa aactggtttt tcagttcatt
    17341 catatgatac tagttatgag ccatatcctt ttacaggtta ttatattgct tgtttcgcat
    17401 aataaattat attattaaaa ctaggaggat gtatggaaga aattagcata aatctattat
    17461 gtgcagttgc aggagttgta atatcctact tagcatttag aaatagctca aacagaaaga
    17521 tacaagatga tacagaaaca actacaaaat tagaacaaca aataactttt ctgtgtgaga
    17581 atgtaagaga tataaagcat gatgtagcaa agtttaatac aagtttctta gatatcagtg
    17641 aacgagttgc aaaagtagaa gcaagtacaa aacaagcaca tcttagaatt gatgaaatta
    17701 taaatagaat tggaggaaaa taaaagatgg ataatttaat aagttttata ccagagcagt
    17761 tactaatttt agtagctgct ctttatgtta taggagcagg ttgcaaaaaa tataaacaat
    17821 tagataataa atacattcca gtagtgttat tgatacttgg aataggtttc tcaatatgga
    17881 tgctaggatt aaatcctgtt gcagtcttac aaggtgtaat ttgttgggga gttgcaatag
    17941 gtgtaaatca aacttacaaa cagtttaagg atggtgaaaa gtaatgaaat taacaaaaat
    18001 attattactc acaaaaattt aaaaaatttc attaaataga gtatactttt atagctaata
    18061 agtatataat aatagtacag aactcgtaaa gcatttatat gctcaaatat acgggacatt
    18121 gatttttgaa ggaacccgcc aagcttcttt tatagctcaa atagtcggga cagataaatt
    18181 tacccactaa tattaattgg tgggttttat tatataaaag tatatctatg aaaaggtgaa
    18241 aacaatggtt gaagtaaaag aagaaaaaac atttgatgaa caaatagata ttttaaaaag
    18301 tagaggatta ataataaatg ataaagaaga tgctaaattt gtattaagta atgtcaatta
    18361 ttataggttt acagcatatc ttctaagttt taagaatgat gatggctcat ataaagaagg
    18421 aactacgttt gaagaagttt atgatatata taggtttaat aaggaattta ggatattatt
    18481 aacagatttg ttagggagca tagaaatagc atttaggaca tacattgcat atacattagc
    18541 aattaaacat ggtgcttgtg gatatctata aagggagagt ttcaaagatg aaaaattcta
    18601 tattaatttt ttgacagcat tagagagaga aaaaaataat aattcagata agctttttat
    18661 tatacaccat aaagaaaaat ataaaggaaa acttcccatt tgggttgcaa cggaaataat
    18721 gacttttggt atgttgtaaa aattatattc aaacatgttg ccagaagata ctagatacat
    18781 aaaaaataat ttgtgtagag tgaatacttt attagttaaa tcttggttac aatcattaac
    18841 acaggtcaga aatcaatgcg ctcattatgg tagaatatat aataataatt tccgtattat
    18901 aacaataaaa aacgaatata aaaagtataa cttggataat aaaaagatat tttcttatat
    18961 acttgctatg aagcatttga ctatggataa attaatttgg aatagttttt ttataaaact
    19021 tcaaaagtta attaatgatt ataataattc tatagactta aagcttattg gttttcctaa
    19081 taattggata gagatattgg ctaaataaaa tagttacttt aagaagttta taaacactta
    19141 ctatatgtaa gtgttttttt attgaaaaga aggaggaaaa taaataatga aaatatgtat
    19201 tacagtagga cacagtattt taaaaagtgg tgcatgcact tctgctgatg gagtagttaa
    19261 cgaataccaa tacaataaat ctcttgcacc agtattagca gatacattta gaaaagaagg
    19321 tcataaggca gatgtaataa tatgccctga aaagcagttt aaaactaaag cagaagaaaa
    19381 gacttataaa atacctagag ttaatagtgg aggatatgat ttacttatag aactacatct
    19441 aaatgcaagt gatggtcaag gaaaaggttc agaagttcta tattatagta ataaaggttt
    19501 agaatatgca actagaatat gtaataagct aggtacagta tttagaaata gaagagctaa
    19561 attagataaa ggattatata tcttaaatag ttcaaatcct acagcagtat taattgaaag
    19621 tttcttctgt gataataaag aagattatga gaaagctaag aaactaggtc atgaaggtat
    19681 tgctaagtta attgtagaag gtgtattaaa taaaaatata aataatgagg gagttaaaca
    19741 gatgtacaaa catacaattg tttatgatgg agaagttgac aaaatctctg caactgtagt
    19801 tggttggggt tataatgatg ggaaaatact gatatgtgat ataaaagatt acgtgccagg
    19861 tcagacgcaa aatctttatg tcattggtgg tgcagcatgt gagaagattg gttctatgac
    19921 taaagaaaaa tttactatga taaagggtaa tgatagattt gatacacttt ataaggcatt
    19981 agattttatt aatagataga ttatataaaa tattttagta tttcttgaaa atcataagta
    20041 tggtaatatt tagataaata ttatttatta ttaataggta tgggggtaga ttatgatatt
    20101 taagaagtat ataaaatcat tactgatagt tgttagtgtt acattgataa tgaacaaatc
    20161 aatattattt gtaaatgcat tagatttaga gagtaacaat aacataaaat cttataaaag
    20221 aaagaatgta aaggaaattg atataagcaa taaatttgtt gatgaatcct ttagaagaga
    20281 tattgttgaa aaggtattag ataataggga tgatccatta acaaatgaag ttgcatcaaa
    20341 agataactat aaaatatatg aaagtgattt aaataaaatt gagagttgct tagagttaga
    20401 tttacataaa aatccagatg tttccaaccc aaacaaaata gataatttag atgggataga
    20461 aaattttaga aatctagaaa agcttgattg tagttcaaat aatctagaaa gtgtagattt
    20521 aa
//
LOCUS       DBGJFU010000070        20384 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.070, whole genome shotgun sequence.
ACCESSION   DBGJFU010000070 DBGJFU010000000
VERSION     DBGJFU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.070"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(90..1046)
                     /locus_tag="ACOIAS_002343"
     CDS             complement(90..1046)
                     /locus_tag="ACOIAS_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGZ2702050.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GHGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            1414..2202
                     /locus_tag="ACOIAS_002344"
     CDS             1414..2202
                     /locus_tag="ACOIAS_002344"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2702051.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(2325..2795)
                     /locus_tag="ACOIAS_002345"
     CDS             complement(2325..2795)
                     /locus_tag="ACOIAS_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HGZ2702052.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            2934..3578
                     /gene="uppS"
                     /locus_tag="ACOIAS_002346"
     CDS             2934..3578
                     /gene="uppS"
                     /locus_tag="ACOIAS_002346"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HGZ2702053.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            3702..4526
                     /locus_tag="ACOIAS_002347"
     CDS             3702..4526
                     /locus_tag="ACOIAS_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2702054.1"
                     /translation="MSKGNNNNNSRNKSKKTSHLNRKKRKLNKKKLAVLICFTVLFLF
                     IAFKATQGVVALVKSMDKSNKTSQQQNVNSEQFDLGNEEENKKKKYTVFIDPGHGGND
                     KGTESKTSNRYEKDLNLQIAKKLANKLSKQKDIQVVVSRTDDTYISLKDRAILANNSS
                     ADVLVSIHLNAEKNGNTATGIETWYRNKATDGSKELAQTVQSTIVSYVKVRDRGIVEN
                     NFEVLRESNMPAILIECGFLTTPSEEQKIINEKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            4841..6388
                     /locus_tag="ACOIAS_002348"
     CDS             4841..6388
                     /locus_tag="ACOIAS_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2702055.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            6391..6789
                     /locus_tag="ACOIAS_002349"
     CDS             6391..6789
                     /locus_tag="ACOIAS_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGZ2702056.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            6829..8562
                     /locus_tag="ACOIAS_002350"
     CDS             6829..8562
                     /locus_tag="ACOIAS_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGZ2702057.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            8794..10005
                     /locus_tag="ACOIAS_002351"
     CDS             8794..10005
                     /locus_tag="ACOIAS_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGZ2702058.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            10198..10458
                     /locus_tag="ACOIAS_002352"
     CDS             10198..10458
                     /locus_tag="ACOIAS_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGZ2702059.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            10812..12524
                     /gene="ptsP"
                     /locus_tag="ACOIAS_002353"
     CDS             10812..12524
                     /gene="ptsP"
                     /locus_tag="ACOIAS_002353"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGZ2702060.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            12847..14397
                     /locus_tag="ACOIAS_002354"
     CDS             12847..14397
                     /locus_tag="ACOIAS_002354"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HGZ2702061.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            14423..17125
                     /locus_tag="ACOIAS_002355"
     CDS             14423..17125
                     /locus_tag="ACOIAS_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2702062.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQITKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(17631..18296)
                     /locus_tag="ACOIAS_002356"
     CDS             complement(17631..18296)
                     /locus_tag="ACOIAS_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702063.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            18640..20070
                     /locus_tag="ACOIAS_002357"
     CDS             18640..20070
                     /locus_tag="ACOIAS_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HGZ2702064.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         8225 a         1962 c         3381 g         6816 t
ORIGIN      
        1 taaaataaaa acacacccca taaatacaaa gtaatttgca tttaagaggt atgtatttat
       61 catcaaatta ttggtttgtt ttggaatttc taatcttcaa ccataatatc tttaaatatg
      121 aaatcatgca ttgcatctag ttcatattcc tcaaaaggta ttacatagcc tgcattacct
      181 atttttctac cattttcagt agaaagagga aactccatag atttcaaatt taaatttcct
      241 atacttaaaa gttcttttcc taaagatatt atttcattag gattcatatt agtttttaca
      301 tacggaagta ttgtgttaac tagattagga tatttagtga ctggtaaatc cttaacacca
      361 tttattaaac tctgtataac acttctttgt cttctatctc tttcatctgt actatcattt
      421 tttcttatac gagaaaatgc aagtgtctga tatccattta atttttgctc accagaattt
      481 cttatatatt taagagaacc tttattgtca tttttattcc atttataagt ttcaggaata
      541 aatttattta attctcttat ttcactctta tcaacagtaa cagtaactcc acctaaagca
      601 tcaactatat ccataaaaga gaaaaagtca actacggcgt agttttgtat atcaagttgg
      661 aagttttctt ctatagtttc tattaataag ttagcttgtc catatacgta ggcatgtgtt
      721 aatttttgtt ctccatgtcc tggtatatta acataagtat ctctatttaa tgaagttagc
      781 ttaagacttt tatttttatt atcaatagta agtatcatca tggcatcaga acgagaaact
      841 tcttctcctg gtcttgcatc tgtacctaac aataatatgt ttgtgatatt ttcttcgttt
      901 ttatagtcat tactactaag cgcctctgta tttatactat catcttgtat agtttttaat
      961 ttagtaaaaa catatccata tatactaaca ggaaatgcta taactaaaag ggctagaaat
     1021 ataacgaact ttttgagtcc tgacaataat atcccctact ttctaaattt tttaattaca
     1081 catagttaat tatattaaaa atttgttaca taagcaataa aaaacacaat aaattagcat
     1141 aagtttgtca taatataatt atttttatag cattttatag aagaaagtgt gtagttatta
     1201 ttgattttat tggaatataa ttgtatgaag acttaaattt ttataaacta atgtgtatat
     1261 tgaaaaaaat attttcctga atgtagttat agcatcttat gaaaggtgca aattgtaatt
     1321 taaaattttt agtgtataat aagaattgaa aattcaaacc gataaaaatt aataaaaaat
     1381 aattataaat taataatttg ggggaggaaa tgtatgataa aattaatagc aacagattta
     1441 gatggaactt tgttagatga aaagagcgaa ataaatccag agttttataa agtatttaaa
     1501 aaattaagag agagagggat aatgttttct gctgctagtg gcaggcagta tcaaaatctg
     1561 ataaaaaaat ttgaagatat aaaagatgat atgatgttta tatcagaaaa tggaacacta
     1621 gtagtatata aaggaaaaga aatattatca aatcctttaa ataaagaatt agtaaatgaa
     1681 atcatagaga caacaagaag tataaaaggg aaaaaaattg taatgagtgg gaaaaaatac
     1741 gcttatatag aaagcaaaga tgaagcgttt atacaagagg ttagtactta ttatgcaaag
     1801 tttaaaattg tagaagattt gacgaaagta gagggagaca tattgaagat agctgtgttt
     1861 gacttcaaag gtgcagaaca taataataat atctactttg aaaagttttc tgatagagct
     1921 caagtatgta tttctggagt ggagtggctt gatttaactg caaaaggtgc aaataaaggg
     1981 agtgccataa aaaaagtaca aaaaatgtta gatataaaat atgaagaaac tatggtattt
     2041 ggtgaccagt taaatgatgt ggaaatgatg aaaagtgcct atcatagtta tgccatggaa
     2101 aatgcaaatg aacatttgaa acaaattgct agatttagag caaaaagaaa tactgaaaat
     2161 ggcgttgtag ataagataaa agaggttata aaaattggat agtcatatgt atagatgaaa
     2221 aagattattt tgaagaaatc aaaaaaatat caatgtaatt ttgataaata agtttattta
     2281 tgcgaaaatt acattgatat ttaataaaca tcaataatag atatttattt ttttatgata
     2341 ttcagtattt tgttttctat ttgagttgca ttttcagcag aaattaaata aacataccta
     2401 cctgccttct caattatagt attatttgct atttcagaat tgattttttc tccatgtcct
     2461 tctccaaaag gtgcttgtat catatctttt ttataatttt ctagagtttt atagatttta
     2521 tctacctcgt cagtttttat aacgataata tcttctagag ataatttttc agaagaatta
     2581 attacaaaac cttgaattat gtcttcctct acatcattaa aatagttaaa atcctttgca
     2641 tcaaagattt ttgattcctt tataagtaaa tcagaagatt ttaatgcttt ttttattttt
     2701 gatgtagaaa cgtttttgta atcaccactt gaagaacaac taaccattcc taatgataag
     2761 attatcgaaa aaaacaatat taaaattttt ttcatgataa acctccaagt tttttaaact
     2821 ctgtcttaaa ttagtttact acataattat ataaaaataa atgtatttaa tgttacagtt
     2881 ttgattaata atgtaatatt ttgataataa tacctattga gaggtgatta gaaatgagaa
     2941 taccaaaaca tgtaggaatt ataccggatg gaaatagaag gtgggcaagt caaaatggta
     3001 tgacaaaaga taaaggatat gaaagtggaa tagaccctgg actagagtct tatagattat
     3061 gtagagattt aggctgtgaa gaggtgactt tttatggatt tacaactgat aatacaagac
     3121 gtccatcagt gcagacaaaa gcttttacaa aagcatgtat tgatgctgca aatatattaa
     3181 tagatgaagg tgctgcttta ttagttgtag gaaatacaaa atcgccaatg tttccagaag
     3241 aattattacc atatactaat agaattaaag ccaaagaagg tcaaataaaa gcaaatcttc
     3301 tggtaaatta tggttggtat tgggacttaa ataatatatc aggagtagaa aatgtaaata
     3361 aaaataatat acaggattac atacagtcat ttgatgtatc cagactagac ttgataatca
     3421 gatggggtgg aagaaggaga ttaagtggtt tccttcctgt acaatcaatt tatgcagatt
     3481 tttatgttat agataattat tggccagatt ttaagagtga cgatataaag gacgcattta
     3541 gttggtatga tacacaagat gtcactttag gtggataatt aaaaatattc tgatttaagg
     3601 ttgtagagtg ttttaaatta ttgaaaaaaa aacttcttga tggtaaaata acactaataa
     3661 tatgtaaaag tgtaaaaatt tataccaaga agggtgaaga aatgtcaaaa ggcaataata
     3721 acaataattc aagaaacaaa tctaaaaaga ccagtcatct gaatagaaaa aagcgaaaat
     3781 taaataagaa aaagttagca gtgttgattt gctttacagt tttattttta tttattgcat
     3841 ttaaggctac tcaaggtgta gtagctttgg taaaaagtat ggataaaagt aataaaacta
     3901 gtcaacaaca aaatgttaac tcagaacaat ttgaccttgg taatgaagag gaaaataaaa
     3961 agaaaaaata tactgtattt atagacccag gtcatggagg caatgataaa ggaactgaaa
     4021 gcaagaccag taatagatat gaaaaagacc taaatctaca aattgcaaaa aaattagcaa
     4081 ataaattatc taaacaaaag gatattcaag tagtagtatc cagaactgat gatacatata
     4141 tatcattaaa ggatagagct atcttagcaa ataatagttc tgctgatgtg cttgtttcta
     4201 ttcatttaaa tgcagagaaa aatggtaata cagcaacagg tatagaaact tggtatagga
     4261 ataaagctac agatggttca aaagaacttg cacagacagt acaaagtact atagtatcct
     4321 atgttaaagt tagagatagg ggaatagttg aaaataactt tgaagtactt agagaatcaa
     4381 atatgcctgc tatattaata gagtgtggtt ttttaacaac accttcagag gaacaaaaaa
     4441 taataaatga aaaatatcaa gaccaacttg cggaaggaat tgttcaaggt gtactttcat
     4501 acctagattc taaagggaat aagtaaatta actttacttt aatgtgagga taaatttaaa
     4561 agtttaactg tgtaaattgt attaacttta tttcgtaatg ttggaaatat tgtttgaatt
     4621 tagaaactat gaaattagaa attattttat ctatatctaa tttcatagtt ttttttgcaa
     4681 aaaatttata gttataaaat tttaaaaaat aaatttagta taatgtaatg tagttattta
     4741 tatgtgattt taacttgcat tgatttgcta tcaattgtgc tttataatta gtatatataa
     4801 agtttaactg taagataatt taaaaaatag gggaatttat atgaataaaa atagcatgac
     4861 aagaatttac agtttaaata aaataatttt tattataatt ggaattttag gaatagtttt
     4921 actcacaaga catattgagg tagatattaa agttttaatt ttaattttat taaccttgtt
     4981 tagtataaat gtatttattt catatcttaa aattaagttg tatgaggata aaattaattt
     5041 aggattgata aataattcag ataagtttat aaatattact aaagatgaga acttcctaaa
     5101 acatgttcaa aattatatta tcagtaatga aaaagaaaac tgtgtaatgg cttgttttga
     5161 tttatgtaga ctcaaactta taaatgatat atatggttat gagctagggg atgaagtgtt
     5221 aaataatatt ttaagtaact taaaaaatta ttttggaaaa gaagctattt atggaaagtt
     5281 aaaaagtgat gtttttgtat taataataaa gcttgaaaat agggaacaga agattcctta
     5341 tttagtaaat cttattaaga ataaaattgt gatactaagt cagattgaca gttttaaaat
     5401 ggatataaat attgaggctt ctattggaat ttataaattt aacaataatg aaattgatat
     5461 aaaaaaagct atagataatg ctgatatggc aaggcttaaa tcaaaaggac taaagcacat
     5521 agagtatgta gaatttgata atgctatgga agaagaaata caatcaatta tgaaaataga
     5581 gagagattta ttcttggcta ttaaaaataa agagtttgta attcactatc aacctaaagt
     5641 tgattcaagt acaggtaaca ttataggttc agaggcattg ataagatggt tgcacccatc
     5701 attaggcatg gttggaccaa ataagtttat acctatagct gaaaaaaatg gtctgataaa
     5761 caatattggt agatggctaa ttcaagaagt ttttattacc attaacaagt ggatttcaga
     5821 aggtataaat gtcttaccag tctcaataaa tctttctaga gtagaactat ataaaaatga
     5881 tttagttgat ttttttaaac tgatgtttaa tacatataat attccaaaag aacttataga
     5941 attagaaatt acagaaacga cagcactaag agatgtaaaa tttataagtg aaaggcttta
     6001 tgagattaaa tctttaggta tgaatatatc attagatgat tttggagtag gtaattcaaa
     6061 ttttatcaac cttaagggga taccactaga tattataaaa attgataggt cacttatatt
     6121 ggatatagtt acaaatacaa aaacaaagtt tatggtgaaa gcaattgtta acctttcaca
     6181 tgatttaaac gtaactgtta tatgtgaagg ggtagaggat atgcatcagg tcaaagtctt
     6241 aagtgaactg ggatgtaatt tcatacaagg ttatgtattt tttaaacctt tggatgagat
     6301 gaattataaa aaacttctta ctgacggaag ttatataaat ctgaaagata ctcttttaga
     6361 taaatgcatg acagttgagg aggagtaagg atgatttcag aagatgtaga ttattcaata
     6421 agaatcataa gagctatatt taataatgga gaagataaac caatagatgc taaaatgata
     6481 ttaaaaagtg aagatatacc aatactaaca ctatacagac tgcttagaaa gctttgtgca
     6541 aacaatattc ttacttttaa caaagaagat ggcaagacat acatgttaaa tgtgtcattg
     6601 gatacaataa gtctaagaac tattgtagaa gtaattgatg gaaaaatata tataaataaa
     6661 tgtacgagat atgtatctaa ttgcagtaat tgcttaaaag gttgtaaagt aaatcgtgaa
     6721 ttgctagaaa tagaaaaaga aatttataaa cttttatcga gaaaatctat aagacaaata
     6781 ctagaataaa aaattttcag ctatgataat aattggaggt tttcggttat ggatgatata
     6841 tctcaagata attttctact gtctaaagag tatgagaata gcttagatgt agatactaaa
     6901 aaagcaagtg gaatatatta tactcctaaa ataattgtag attatattgt gaaaaaaact
     6961 ttaaaaaatc acgatataat taaaaatcca tatccacgaa tacttgatat ttcctgtggg
     7021 tgtggaaatt ttcttttaga agtatatgat attttatatg acttatttga ggaaaatata
     7081 tatgaattga aaaagaagta tgatgaaaat tattggactg ttgataacat acaccgtcat
     7141 atattaaact attgtatata tggtgctgat attgatgaaa aagctataag tatattaaaa
     7201 gatagcttaa caaataaaaa ggtagtaaat gatttagatg aatctgatat aaagattaat
     7261 ctattttgtt gtgatagtct taaaaagaaa tggcgatata agtttgatta catagtgggt
     7321 aatccaccct atattggtca taaaaaactt gaaaagaaat ataaaaaatt tctcctagaa
     7381 aaatatagtg aagtttataa ggataaagct gacttatatt tttgttttta taaaaagatt
     7441 atagatattt taaagcaagg agggattggc agtgttataa ctccaagata ttttttagaa
     7501 agtctatcag gtaaagattt aagagaatat attaaatcaa atgtcaatgt tcaagaaata
     7561 gtggattttt taggtgcaaa tatatttaaa aatatagggg tatctagttg tattctaact
     7621 tttgataaga aaaaaacaaa agagacatat atagatgtat ttaaaatcaa aaatgaggat
     7681 atatgtataa ataaatttga aacattggaa gaacttctaa agagtagcaa atttgaacat
     7741 tttaatataa atcagagatt gctatctgac gaatggatac ttgtaaataa agatgatgaa
     7801 actttttata ataagataca agaaaaatgt aaatatagct tggaagatat agcaataagt
     7861 tttcaaggaa taattacagg gtgtgataaa gcgtttattt taagtaaaga tgatgtaaag
     7921 ttgaaccttg tagatgataa atttttaaaa tgttggataa aaagtaagaa tataaataaa
     7981 tatatagtag ataaaagtga atataggcta atatactcaa atgatataga taatgagaat
     8041 acaaataaaa ggatactaga tgaaattata gggctatata aaacaaaatt agaaaacaga
     8101 agagaatgta aaagtggaat aaggaaatgg tatgaacttc aatggggtag agaaaagctt
     8161 ttctttgaaa gaaaaaagat aatgtatcct tataaatcaa atgaaaatag atttgctata
     8221 gattatgata ataatttttc aagtgcagac gtatattctt tttttataaa agaagagtat
     8281 ttagataaat tttcatatga atatctagtt ggaattttaa attccagtgt ttatgataaa
     8341 tactttaaaa taactgctaa aaaaatgagt aaaaatatat atgattacta tcctaataag
     8401 gttatgaaga taaggatttt tagggataat aattatgaag agatagagaa tttatcaaag
     8461 caaataatat caattttatt aaataagtct atagataaag gaaaagtaga aaaattgcag
     8521 ataaaaatgg ataacttgat aatggattct ttaggtatat aagcagttgt aagtattaaa
     8581 aaattataat cttttaattg tttatttaaa tttagaaata aaaagttagc catataaaaa
     8641 atattaaaaa tatatacttt tcaatatttt tttgataaaa taagaatgaa tttgtgcaat
     8701 tttatttttc tccaataact aaaaatagtt ttttgaatta atatttgctt aagaataaaa
     8761 atcagaaacg tttttgatat agtaggtgat aatatgaaag gattagtttt agaaggtgga
     8821 ggaacaaaag gagcctacca aataggtgca tataaagctt taagagagct tggtatagag
     8881 tttcaaggag taactggcac atcaattggt gctttaaatg gagcatatat aattcaaaat
     8941 gatattgaag taatggaaga tatttggtta aattatgact atactcattt tatgaatatt
     9001 gatagagaaa cctatgagag gtttaaaaat gtagatttca ctactaaaaa tctcaatgca
     9061 gtgtttgagc ttataaataa agctagaaaa aatgaaggta tagatataag cccattaaga
     9121 caattaatgg agacaacatt aaatgaagac ataataagga aatcaaaaaa agactttgga
     9181 cttgttacgg tctggtggga taaaaaaatt aatccatatc cattgtatat cgaagatata
     9241 cctaaaggaa gattgattga ttatttaatt gctagttcca gtttgcctat ttttcaaacg
     9301 aacatgatgg atgataagtt gtatcttgat ggaatgtttt gtgataacat gccaatagga
     9361 ctcttggaag acaaaggtta tgaagatgtt gttgtaatta ggttgattga tgattttatt
     9421 gggaaaatga atctaaatcg acatcaaaat ataaatataa aaactataat accatcagaa
     9481 tatctaggag gttctcttaa taaagataga gataatgtag aaaaaaacat ccgactaggt
     9541 tacttagata ctatgaaagc atatgaaaga tgcactggaa ttaagtatta ttttgatgta
     9601 gagtataaat ataatgaaga atattgtttt gataaattta gaagtatgag caatgaaaca
     9661 atacaatata tctgtaattt attgaatata aaaagagatg cttcacttag aactgttatg
     9721 gaaagtgtaa taccaaagct tggtgaggtg cttggattgc caaaggattt ttcatataaa
     9781 gatttgtttt actgtatata tgaaaagaaa cttgaagaaa ataatataaa caggataaaa
     9841 ttatatgatt ttaacaaggt tatagatgtt gtaaatacaa atattgattc taatagaaat
     9901 ttagcttcta attttgaagt tagacctaaa attagtatta aaaagaacaa aaacacaaaa
     9961 ttattgacaa attgtattat aaaagacata aaaaatcaaa gttaaataaa aaacaaaaaa
    10021 aaatttgtaa ttatactcga aaaaagtgta tattagtagt ataatatttt tgtagcacta
    10081 gtgggaacaa aaaatgacca taggacaaaa aagtaccact tagtaccatt aaagtaaaaa
    10141 acaggaaaac aattttaaaa aatataaaat gtaaagttta taggaggatt taaaaaaatg
    10201 gaaaaagtag ttagtatcaa aaatgcaagt ggattacatg ctagaccagc aggaatgttt
    10261 gttaagaagg cagctgaatt taaatcaaca gtagaggttg tagcaaaaga caaaactgta
    10321 aatgcaaaat caataatggg aataatgagt ttaggtttag ctcaaggaga agaattaaag
    10381 ttagttgtaa atggagaaga tgaagaagta gctttagctg caatggttga attagttgaa
    10441 agtggatttg gagaatagat ataataattt tttaaaaatt tgatatttag aatctaatat
    10501 tacttaaagt aaagtaacag agaagtatta aacatataca gaccaacatt tgtagatatt
    10561 taatttcttt gttactttca tgtttattta atcagaagat taagactatt gttaattggg
    10621 tataaaaata taaatatttt agaaaatgaa ataagatatt tttaatcata aattcagagt
    10681 aaaaatacag taacaaatag tttataaaat tttataaaaa attataagta aataaaacta
    10741 taaaaatgta aattatttat ataaatttat aaagtttatg tgctagatga tattttttag
    10801 gaggtagagt tatggcttac aaaggaatag gtgcttctcc tggtgttgct ttagggaaag
    10861 cgttagtagt tgaacatagt gaattagtaa ttgaaaaaaa atctatagat aatgttgaag
    10921 ctgaaattgc taaattagaa gatgcagttg cagtttctaa agaagaatta gtaaaagtta
    10981 aggaaaaggc ttctgaagaa ttaggagcag aggaagcaga aatatttgaa gcacatttat
    11041 tagttttaga agacccagag ttaattggtt ctgcaataga taaaattaaa actgaaagtg
    11101 taaatgcaga gtatgcttta aatgaaataa aagagatgtt tgttagcatg tttgagtcta
    11161 tggacaatga gtacatgaaa gaaagagcgg cagatataaa agatgttaca aatagaatac
    11221 ttagacatat attaggaatt aaagttgttg atttatctgc tttaagcgaa gaagttgtat
    11281 taatagcaca tgatttaact ccatcagaca cagcaactat gaataaaaaa atggttttag
    11341 gatttttaac tgatataggt ggaagaactt ctcatactgc tatcatgtct agaactttag
    11401 aaatagctgc aatcgttgga ttaaatgata taacttcaaa agttaaggat ggagattttg
    11461 tggttttcaa tggagacaca ggtgaagtaa tcgtgaatcc tgatgaagag actataaata
    11521 aatatactga attaaaagct aagtatgaag atgagagaaa agcattacag ctattaaaag
    11581 gaaaaccatc tgtaacttta gacggaaaac atgtggaatt agctggtaat ataggaacac
    11641 ctaacgatat agaaggtcta ataaagaatg atgctgaagg tgttggtctt tatagaacag
    11701 aatttttata catggataga gattctttcc caacagaaga aatacaatat gaagcataca
    11761 aagctgtatt agaaggaatg gatggaaagc ctatagttat aagaacattg gatataggtg
    11821 gagataaaga attaagttat ttaagtatgg aacctgaaat gaatccattc ttaggttaca
    11881 gagcaataag actttgccta gatagaaaag atatatttaa gactcaatta agagctttat
    11941 atagagctag tgttcatgga agattaagaa tcatgttccc aatgatttca tctttagaag
    12001 agttgttaca agctaaagaa gttgtaaaag aagttttagc agaacttgat tctgaaggtg
    12061 tggcttatgc taaagatgta gaaattggta tgatgataga agttccatct gctgctgtaa
    12121 tatctgatgt attggctaaa cacgtagatt tcttctctat aggaactaat gatttaattc
    12181 aatacacttg tgcagttgat agaatgaatc aaaagataag ctatctttac aatcaattta
    12241 atccagctgt tttaagactt ataaagactg ttatagacaa tgcacataaa gaaggcaaat
    12301 gggcaggtat gtgtggagaa tctgctggag accaaaaaat gataccaata cttttaggta
    12361 tgggattaga cgagttctct atgtctccaa tatcaatact tcctgctaga aagttaataa
    12421 cttcagttaa agagtcagat atgaaaaaat tagctgatga tgtacttaat atgggtactg
    12481 ctgaagaaat aaaaagctat atagaaaaga cttttaatat ataattaatg ttatatagtt
    12541 actaaaattc atttttctaa ataacaaaat tatattttac gaataaaaaa actatagtat
    12601 atcatatata ctatagtttt tttgttatat aaattacgta tcaaaatttg taaaatgaat
    12661 agatataatt actaaatatg tgatagtatt ggctataata ggatataaaa aataatattg
    12721 taaaaaaaag tttatattta gtaatgaaaa tttaacgttt tacttgaaat tatgtgtttt
    12781 gtaatgtatt attaaggctc taataggaac attttcatca atttttctat agtgggggta
    12841 ggaattatga ggtacaaaaa aagaaatata gataagtcaa gtgaactttc atttcaatct
    12901 ctagcaaaat attttataac tttctatata gtaattatat taattattct ttctatgttt
    12961 tgtataaata caatatgggt tatgaaaaaa gataatcaag atttattatc tacaacatca
    13021 aatcgtattg aagatagggt aggagaaaca attaagctat tgaactcttt gagtaaagat
    13081 aattacataa tagataatag tgtgaatggc atagataaaa gtaaagaact ttcaatgtat
    13141 agtgatgaat ttgggtatat gatgatatgt tttgttgata agaatataat tgtgtgggat
    13201 gaagaaggtg gtaatattag tctagctggt cgtgattata tgcagaaact ttttgcaaca
    13261 aaaaaatatc aagttacaga tagttttgca gcaggggctg atggaaaaac tttaaactac
    13321 actattgcat cacctataat aaagaatgga aatgtagatg gttgtgtatt tgcagcaatt
    13381 tactttgatg agataaagga tatactcaaa caaggaaaag gtcaacagca acagaatttc
    13441 atattatttg gaagtgaaaa tcaaataatg aatttaacaa gtgatgaaac tgcatatgga
    13501 gagaagtata tgaagttatc gcagaaaaca aaatatcttg gaaccaatgc aaaaaaagta
    13561 gaaaaggata tgtttaataa taaatcaggt gattattggg cttttgaaaa tggaaaatta
    13621 tactttatgt cctataaacc aattaagaac acaaattgga gtttattttg taaagttaat
    13681 ttcttagata tttttgcatt aaatttaatt cctatgggag ctattctatc tgttataact
    13741 ttagtattta ttattttgat ttgtatggag aaaagcttca tgaaaaaacg aatggaagtt
    13801 attgatatgc ttttaggttc tgttcaagaa ttagaaaaac gtatatatca aacagaaaga
    13861 cctagtgaag tagactttaa agaaattatt cagctcacaa gtaaaggtct gactgatgga
    13921 ttaactggtg ttgtaacacg tactgtattt tttgaccaaa tttataatcg tgttgaaatg
    13981 gaggaaaatg ataaattatc tgtaatttca tttgttgatt tagatgattt gaaatgtatt
    14041 aacgacttat atggacatga aattggggat atggttttga aaaatgttgg atatgtactt
    14101 cgtgaatatg agataaaata taatggttta attgggcgct atggtggaga tgaatttgtt
    14161 atgttgttat ctggttttac atcgagagaa gaggtggagg atttattgga tgagattgct
    14221 attcgcttac atactgaagt actaacagat gatggtatca ttcaaactca ttgtagtgtt
    14281 ggagctgtag tatgtagtgt aaaagacaat aaagatatag ataatttatt aaaacaggct
    14341 gatgaagctt tatactatgt taaacgtaat ggaaaaggta attatcattt agtttaatgt
    14401 atgtggaagg agtttatatc atatgaaaaa tgtatctaaa aaattttttg tatgtactat
    14461 tattgtatta ataacatttt tatgttttag taaatttgct atttatgttt ttgacaaaca
    14521 agaaaagaaa gaaacaaaag tagtagaaaa catgttaaat gtctacaaga ataacattat
    14581 tttaaatctg actgaaaaat ttaattatag caaagacttg acatcagttt tagtttcaaa
    14641 caattattct atagataaat taaaacctat tgcagatgaa attcttaaaa acaattcttg
    14701 catatatatt tcagttgtag aaaaatataa tgtagctgca tttttttcta attctgtcaa
    14761 aaaagatatt atcaatactc caatacagga actgaattat tcttattcat tagcaaattc
    14821 tatcaaagga ttagtcgtag aaggtccttc taaagaccag ttttcaaatg gagatgtgtt
    14881 tttaattact aatcctattt ataaaaatgg agagtactgg ggagaaatta cagtagcttt
    14941 agatgccaaa aaagttgtag attcttttaa cttaaataaa ttaaaagaat ctggatttga
    15001 gtatgaactt tggcgtgtac acctagataa tggttcaaaa tctacaatag aaatttctac
    15061 taaagatttt aatttttcca aatcacatga aattaagttt aaacttccga ctgtttggac
    15121 tcttagcatt gtgtacaaaa atggatggaa taataagaat atagatttta ttgtatttgt
    15181 tatattgtta attatttcta tattaatagg tgtgatgaca tttatttaca ttgaaaagaa
    15241 gtcgattagt aaggaattaa atcaacagag tcttaaaaat ttagaaactg gtattttgaa
    15301 tagagaaggt tttttaatta agttgcaaga gcatctgaat agaaaaaata atgaaatagt
    15361 gttgctgtat atatgtatta ctaattatag taaatttgct ccagttatga gctttgaaaa
    15421 aagacaagat tatcttacac atatagtaaa aaatctagat aattacataa aaagttctca
    15481 ttttattgct cgtataagtg aggaaggttt tgttctagct ttagaagaaa attgtgataa
    15541 aaatgaattg cttaacattg aaaaagggat ttccttagag cttttacgaa aaattaaagt
    15601 agaaaataaa caagtatttt taacgattgg ttatggctca tctaggtgtg ttgatgatag
    15661 aaaagatgca gaatctctgt tactttcagc tattgaagat tatgagagaa gatttccagc
    15721 aatgtctcct attcatgatt ttactaaaaa gtgtgaagat atgcttgatg aaaagaaaca
    15781 gattgtattt ggtgaatatc cagatttaga aataaacaag ttgtctaaag taatatacaa
    15841 atattataaa aaagtacagc gtattatgta taaagattat aatattgata ttggaaatag
    15901 gcgagggtat atgcgtgatg tcgaagtatt aattgattat aattctgaga gaatgttata
    15961 cttattttta atagacatat gcaagtttgg aaaatttaat gaactgtttt ctattgaaat
    16021 aggagatgtt attttaaaag aagttagtaa caaattaaaa acaatttttt ctaattatct
    16081 atatcgtatt aatggagatg tatttttagg catttcaata gataaagaat cttacttaga
    16141 tactttacat aaggttaatg aagtatttaa tagttcttta agtgtagaag atgtactttt
    16201 tgatgtaaaa tgtaatattg gtatttgtca atatccatat catggtgaaa atccatatga
    16261 ccttctggaa aaggtacaaa ttgcattgga gtatgcaaaa aaggatgtca aaagtaatgt
    16321 taaagtttat gataaggaat tgacagaaga gatttaccat gaaaataata ttattaagga
    16381 tttaaaaaaa ggtctggaag aacaaacact agaagtttgg tatcagccaa tttttaatgt
    16441 tagtaaaaac tgttatataa aagctgaggc actaattaga ttgagaaata aagagggcgg
    16501 ttattattct gcctatgaag ttattactta tgcagaaaag catggactta ttaaggatgt
    16561 aggtctatat gtactaaaaa aggcttgtga tgtaactaca aaaataaaag ataaaaatat
    16621 agactttgaa caaatgcaat taaatttatc tgctcagcag attgtacata agcagtgtgc
    16681 aaatgacata ttatcatgta ttgagaattg tggtctagat aaaaagtata tttctgttga
    16741 agttacagaa aaagtactta tggaaacttt tgaaattgta aatgttactc ttgataaact
    16801 aaaaaaagaa ggcattagca tagctttaga tgagtttggt tctggattta gtagtataaa
    16861 ttatctggca aatttaccta ttgaaaattt aaaaatagat aaggaattag tagaacaaat
    16921 tacctattct aaaaaacaat ttgaattatt aaaaactata gttcaaatta ctaagattaa
    16981 tggcatggat attgtagcag aaggtgttga aacaaaagaa actctggatt tagttgtaac
    17041 ttcaggtgtg aatttgattc aaggtcatta tttttcagaa cctttaagtg aagataaatt
    17101 tattgaactt atgattgaag aataaaggtt aataaaataa agatttaagg tattgaaaaa
    17161 taattaaaag tatttaataa tagaattagg gaagaataag tttataagta aaaacaattg
    17221 attagtagtt aataaaataa atgtgatatc caaataaatc ctaaatctaa tttgagtttt
    17281 aatatagata gtctaaagtt tgattattaa agatttaatt gtaagttgtt acagttgatg
    17341 atattgatta gagattaaag tttagagtag aaagttacct taaatttaaa aatgcaagat
    17401 aacccaatct aaaaattata atcactcgat aggtttaagg atttatttgt ttattataaa
    17461 taggagggtg tctcaaaatg aactttttag tttatgaggc actctctttt atatagaatt
    17521 aaatatattt tattatttca acaatgaaaa agaagcttat ctctattttg agacagcctc
    17581 tttgtatact attattaatt taatcaataa cataatttaa aattataata ctagttaaga
    17641 tttcctgctt tattagtaaa taattcttta atagcattgt ttctggaaac taaatcgtta
    17701 tcagagttgt ttgttttttt cataatcact gaataacgtt ttttatttat aaaaaatatt
    17761 aaaatcttat catcatcaaa attgtattct ttatatctgt cgatagactc ataacctaga
    17821 tttagaatgt ttttgtcttt tatactatct acatctaaaa tatatgatgg gtcagccttt
    17881 agtaatatat taattaaaca tttttcttca ccagcacaga ataactccat cttacttgat
    17941 atagatttag attgctctga agttattttt gcaggaataa ggttgtcatt atcatctctt
    18001 agaagataac catgtgtgtc ttgttgatat actaaattaa cctttttctt aggaacacta
    18061 atcattttat ctaaatgtac ataatgatga ttttcaacaa aaccttcatt taatatttca
    18121 acaaagttat taattttacc taggcaaaaa gattctatag attctttaca gaatttattt
    18181 gtaaatggta ttatgtttac gttttcctcg tctgtattcc atgctatagc ataatccaac
    18241 gaatatactt cttcctctat atctgcaaaa ttatattgta ctatgtttcc tttcaaaatt
    18301 actcgacctc catcttataa tatatttaat atactttata aaacttatat tatatttaaa
    18361 taatatcata aattgaataa atatgatata gtaaataaga aaaaatttga aaaaaatcac
    18421 tataaatgag taataaatat aaatagtgta tactaatggg atggaaaatg tccactatta
    18481 actttattac cccacatata taagataaaa ttattaatat aaaatataat ctatcgacaa
    18541 tgaatagact tttaaataaa cttaatataa aattaattaa ataagtgctt aaaaacgatt
    18601 tactttaatg atagaatcat attaaaggag gataatttta tggatataaa gaataaaatt
    18661 aaatctatat ttttaccaga aaaaaatgac tatttagatg atgaagaatt tgagtatgat
    18721 atagaaaata atgaagaaga tttaaaggaa gataacaaag tacatttaaa tgatttaagc
    18781 agagtaaaat atgattacag agagttaaaa gacatagaaa accaaacaga agagatgtgt
    18841 ctgctagcag taaagcagga tggtactata attgagtttg taaaggacaa gacttataaa
    18901 gtatgtatgg aagcagtaaa acagacatat aaatctctta agtatataac aaatcaaaat
    18961 gaggatattt gtatagaagc agtaaaacag aactacagag ctttgtacta cataaataat
    19021 aagacagaaa atgtactaat tgaggctata aaaaatgcat caacttatga tgtcatggaa
    19081 gtatttaaat ttgtggaaga gcaaacagaa gatgtatgtc tagcatttat agaaagggca
    19141 tctaaaaatg atgttgcaga gatattaaaa ggaataaaag agcaaactcc agccatatgt
    19201 ttagaagctg taaaaaaaga tggaaaatct ttagcttatg taaaggaaca aagtaatagt
    19261 atatgtttag aagctgtaaa agaaaactac agtgcactat cttgtgtaaa agagcaaact
    19321 gaagaaatat gtatagaagc agtaaaacag aatgactttg cattgtatta tgtaaatgaa
    19381 cagacagaaa aaatatgtat ggaagcagta aaaagaagtt atatggctct acagtatgta
    19441 aataagcaaa cagaagagat ttgtttggaa gcagtaagaa ttgatggaag agcattacag
    19501 tacgtaaaag agcaaacaga agaaatttgc ttggagtcag ttagacaaaa tggaaaagta
    19561 ctgcaatatg taaaaaaaca gactgaaaat atatgtatag aagcagtaag aggttcattt
    19621 gaagaacttg aaataaaaga aatattaagt tatgtaaaga taccaacaga aagaatattt
    19681 gtagaagcag taaagcaaaa tggaaaaatt cttaaatatg tagaaaatca gacagaatta
    19741 atatgtttag aggcagtaag agaaaactat aatgctttag cttatgtaaa ggaacaaaca
    19801 gaaaagattt gtttagaagc agttaatcaa agttatgaag cgcttaaata tgtgaaagaa
    19861 caaacagaag aagtttgtct aaaagcagta aaacaagatt atagaatgtt gaaatatgta
    19921 aataatcaaa cagaaaaaat atgtttagaa gcagtaaagc aaaattatag agctttagaa
    19981 tttgttgata atcaaacaga aaaagtttgt cttgaagcag taaaacagaa tagaaaagca
    20041 ctacagtatg taaaacaaaa acaaagttaa aaggctaaaa tatatagaaa acatatgcaa
    20101 ataaataaaa tttgatattt tgtaataaat aaaatgggta tctcaaaagg aactttttag
    20161 tttgtgaggt actcttttta tatgaaatga aatatatttt tagcctttta acaatgggga
    20221 aaaggtttat ttctattttg acataggctt tttatgcata taaagcttga taaaattgtt
    20281 ttattataat tgttttgtta tataaataat aagaagaaaa ttatataggt ctttttttga
    20341 ccagttttac atttttaaca actagttttg taaagtatat tgaa
//
LOCUS       DBGJFU010000071        20249 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.071, whole genome shotgun sequence.
ACCESSION   DBGJFU010000071 DBGJFU010000000
VERSION     DBGJFU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.071"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     misc_binding    <1..222
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            330..1811
                     /gene="thrC"
                     /locus_tag="ACOIAS_002358"
     CDS             330..1811
                     /gene="thrC"
                     /locus_tag="ACOIAS_002358"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGZ2702065.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1821..2714
                     /gene="thrB"
                     /locus_tag="ACOIAS_002359"
     CDS             1821..2714
                     /gene="thrB"
                     /locus_tag="ACOIAS_002359"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGZ2702066.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            2844..3899
                     /locus_tag="ACOIAS_002360"
     CDS             2844..3899
                     /locus_tag="ACOIAS_002360"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HGZ2702067.1"
                     /translation="MKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVISG
                     VQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEAVK
                     AGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIVVE
                     GPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSSSD
                     VKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAIRN
                     KFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAYKI
                     DKLSTVEDVINELISEL"
     gene            complement(4054..4566)
                     /locus_tag="ACOIAS_002361"
     CDS             complement(4054..4566)
                     /locus_tag="ACOIAS_002361"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGZ2702068.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5086..6021
                     /locus_tag="ACOIAS_002362"
     CDS             5086..6021
                     /locus_tag="ACOIAS_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGZ2702069.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6113..7984)
                     /locus_tag="ACOIAS_002363"
     CDS             complement(6113..7984)
                     /locus_tag="ACOIAS_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGZ2702070.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8174..9100
                     /locus_tag="ACOIAS_002364"
     CDS             8174..9100
                     /locus_tag="ACOIAS_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HGZ2702071.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9239..9385
                     /locus_tag="ACOIAS_002365"
     CDS             9239..9385
                     /locus_tag="ACOIAS_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702072.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9490..10014)
                     /locus_tag="ACOIAS_002366"
     CDS             complement(9490..10014)
                     /locus_tag="ACOIAS_002366"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2702073.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10067..10291)
                     /locus_tag="ACOIAS_002367"
     CDS             complement(10067..10291)
                     /locus_tag="ACOIAS_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702074.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10488..11612
                     /locus_tag="ACOIAS_002368"
     CDS             10488..11612
                     /locus_tag="ACOIAS_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HGZ2702075.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12086..12910)
                     /locus_tag="ACOIAS_002369"
     CDS             complement(12086..12910)
                     /locus_tag="ACOIAS_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5780 domain-containing protein"
                     /protein_id="HGZ2702076.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(13258..14313)
                     /gene="ispG"
                     /locus_tag="ACOIAS_002370"
                     /gene_synonym="gcpE"
     CDS             complement(13258..14313)
                     /gene="ispG"
                     /locus_tag="ACOIAS_002370"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGZ2702077.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(14495..15499)
                     /gene="rseP"
                     /locus_tag="ACOIAS_002371"
     CDS             complement(14495..15499)
                     /gene="rseP"
                     /locus_tag="ACOIAS_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HGZ2702078.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(15512..16666)
                     /locus_tag="ACOIAS_002372"
     CDS             complement(15512..16666)
                     /locus_tag="ACOIAS_002372"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGZ2702079.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(16830..17033)
                     /locus_tag="ACOIAS_002373"
     CDS             complement(16830..17033)
                     /locus_tag="ACOIAS_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702080.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
     gene            17370..17951
                     /locus_tag="ACOIAS_002374"
     CDS             17370..17951
                     /locus_tag="ACOIAS_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702081.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIAKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
     gene            complement(18039..19850)
                     /locus_tag="ACOIAS_002375"
     CDS             complement(18039..19850)
                     /locus_tag="ACOIAS_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2702082.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
BASE COUNT         7086 a         3103 c         2347 g         7713 t
ORIGIN      
        1 tgattaggaa gagtaaatct gaatcgatta ccagagaagg aaactactag ctgaaatgtt
       61 tccctaatta gaaggattga aaactaccta ggagcttcta tccgaagtta tcttaatgat
      121 tttagtagga atagacgttt ttattacttg cgttaaagta tcaagtggat tttttaaaat
      181 caatttgggt ggaaccgcgg gaaacagatt ctcgtccctt ttttagggat gagggtcttt
      241 ttttattacc taaattcagt ttttaaaata ttctgtatct aatttaatta gctaattaaa
      301 aatttaaaat atatggagga ttataaaaaa tgtattattc aagtacgaga ggcaccgaag
      361 aaaaagtaac tgcatcacaa gcaataataa aaggaatatc aaatgatggt ggtctttatg
      421 ttccaagtga atttcccaat gtaaaaaatg aacttataaa tttagtaaat ttaacgtatt
      481 ctcagatagc attttttgta cttagtaaat ttttatgtga ttttactgaa gatgaaatta
      541 aaaattgtat agaaaatgca tatgatgaaa agtttgattg tagtagtata gctccattaa
      601 ataaagtaaa tgatacttat ttcttagaac tatatcacgg tccaacttta gcatttaaag
      661 atatggcact tactataatg cctcatcttc ttaaaacatc tataaagaaa gataatttag
      721 agaaagatgt tgtgattctt actgctacat ctggagatac aggtaaagct gcacttgagg
      781 gatttaaaga tatagataaa ataaagataa ttgtattctt cccagaagat ggtgtaagcc
      841 cagtacaaaa acttcaaatg aaaactcaaa ctggaaaaaa tacatatgta gttggtataa
      901 aaggaaattt tgatgatgca caatcaggtg taaagaagat tttttctgat aaagaattta
      961 atcaaaaact attggataat aattatatat tatcttctgc aaattcaatt aatataggaa
     1021 gacttcttcc acaagttgca tactattttt attcatatat gaaattagtt agcaatagtg
     1081 atataaattt aaatgataaa ataaattttg ttgtcccaac tggtaatttt ggtaatattc
     1141 tagctggata ttatgccaaa caaatgggtc ttccaataaa caaattaata tgtgcatcaa
     1201 atgataataa tgttctttat gatttcttta aaactggtgt atatgataaa aatagaacat
     1261 tgaaattaac tacttctcct tctatggata tattaatttc aagtaattta gaacgattac
     1321 tgtttgaaat atcaaataga aatactgata ttgtaaataa gcttttatca gaccttagtg
     1381 ataaaggagt ctacaaaata agtgatgata tggaaaaaaa tttagcctca ttctatggag
     1441 aatattcaaa tgaagaagaa gttaaaagca cgataaacaa tgtgtttaaa aattataatt
     1501 atttgataga tactcatact gctgttgcat acaattgtta tgaaaaatat aaaaaagaaa
     1561 cttctgataa cacaaaaact gttattgtaa gtactgcaag tccattcaag ttttctgaag
     1621 atgttctaaa gtctatagat tgtgatttta aagaccttga tgactttgct ataattgata
     1681 gattatcaag tatttctaaa atagatattc caaaacctat taaaaatctt aaaaatgcag
     1741 aaatactaca taaagatatt tatgaaaaag atgaattaaa actagctata aaaaaattct
     1801 taaaggtata ggtgattatt atgttagaga ttatagttcc tgcaacaagt gctaatatag
     1861 gtccaggatt tgattgtctt ggaattgcgt taaatatata caataaattt tatgttgaag
     1921 aaattgaaag tggtcttgaa atagaaggtt gtgaagatgc ttacaaaaac gaaaacaact
     1981 tagtttatac atctatgaag tacttttttg atagagttaa accagaaaaa atacctgctg
     2041 gtatcaaaat aaaaatccaa agtgaagtac ctatatgtag aggtcttggc agtagtgcct
     2101 cttgtatagt agctggagtt atagctgcaa atgctctatc aggtgctaat ttagataaaa
     2161 atcaattact aaatatagct tcagaaatag aaggtcaccc agataatgtt gctcctgcta
     2221 ttttaggaaa tatgattgta tctgttactg ataatgagaa tatacattat gatattataa
     2281 aaattcctga agaacttaaa ttttgtgcaa tgatacctaa ttttaagtta tcaacagaaa
     2341 aagctagagg tgttttacca aaggaaatac catattctga tggggtattc aatgttagta
     2401 gagttgcttt actaatatct gccttactaa acaaaaactt tgacctactt aaagtagctt
     2461 gtcaagacaa attgcatcaa gattatagag gaactctaat tgagaactac aatgatattg
     2521 ttgaaaaatc tgaacaatta aatagtattg gagtattttt aagtggagct ggacctacta
     2581 taatgtcttt aataaaagaa aatgatgata gttttgtgga taatatgaaa aattacctac
     2641 aaaaactaaa atctgattgg gaaataaaag agttatgttg cgattctaat ggagctgttt
     2701 taaatataat ataattatcg tcttctatac aaaatataac tactctttta ttataatttg
     2761 tagtataata attaggacta ggtaataata ttaagtatta taatctagtt ctttaaacat
     2821 gtttacattg aggagggatt atcataaaag agttaataat tagagattta gttgcaaaag
     2881 ttcctataat tcaaggtgga atgggcgttg gtatatctag gtcatcacta gctggtgcag
     2941 ttgctaagct tggtggtgtt ggtgtaattt ctggagttca aattggatat gatgaggaag
     3001 attttgaaac taatactata aatgcaaatt taagagctat aaaaaaacat atttctaagg
     3061 ccaaagaaat ctcaaatggt ggtattattg gtattaattt tatggtagct atgaaagaat
     3121 atgaaacata cgtaaaggaa gctgttaaag ctggtgtaga ccttataata tctggagctg
     3181 gattacctaa taagctacca tcattagtaa agggaagtaa tgtaaaaata gcacccatag
     3241 tttcaactgc aaaagctgct aatgtaattt taaaaatgtg ggatagaaaa gagaaaacta
     3301 ctgctgactt aatagttgtt gaaggtccaa aagctggtgg tcaccttgga tactctaacg
     3361 aggaacttga taacatagat tctatagact atgataagga atttgttgaa attttaaagg
     3421 ttgcaaatac ttatggagaa aaatttggta gaaacatacc agtagttgct gctggtggta
     3481 taacttctag tagtgatgtt aaaaaatata tagatatggg tgcaagtggt gtacaagttg
     3541 gtacaagatt tgttgctact tacgaatgtg atgcccatga gaactttaaa atggcataca
     3601 taaatgctag tgaagaagat gttcatatag ttaaaagtcc tgtaggtctt cctggacgtg
     3661 ccataagaaa caaatttata gaagaagtta aaataaatcg tccagaaata aagaagtgct
     3721 ataattgttt gattccttgt aatccaaaag aaactcctta ctgtatttct caagctctaa
     3781 tcaatgcagt taaaggagat gttgaaaatt cacttttatt ttgtggaaat gatgcctata
     3841 aaattgataa attaagtact gttgaagatg ttatcaatga attgatttct gaattataat
     3901 ttaaagaaaa ctaaattaaa attttatcaa gctaaggagt ttaaatacgt tatttattaa
     3961 tctttataaa catatttatg tccttagctt ttttgtgtca atttcctttt attgaaattt
     4021 taatttaatt agagatagta ttaaccctta taactaatct tcttttacaa gtacattggt
     4081 tatttcagct atatataaag tatgataatc tttttgtgga taccatcttc catctatacc
     4141 ttcagcaata aaacattggg gttcaagttt ctgactatac atttttttgc atataatagc
     4201 cattttagct tccttaaaat aaggtgtatc atttaagtgt tctatattaa atccaatctg
     4261 tgatatctta tcctcttctc taccagatac ttttccacaa tatgaaagct ctttacgaaa
     4321 atcttcatca aaaaatgtaa gagaaaattt atctgtatta tctataaatt ccttagtata
     4381 acgctgtggt cttattacta caaaagcaac atcttttccc cacataactc caagccctcc
     4441 ccagctagca gtcattgtat tcactttacc ttcattttct gctgttataa gtgtccaatc
     4501 tttacctatc aatccaaatg gattattttt aatctcttct gccaaaattt ctttaaaatc
     4561 actcatatct ctaataacct ctcattctct tatatttata ttccttttat tttcatgata
     4621 aattataaat gaagtctagt tttgaaaata aaatactttt ttaaatatat tattgcaaat
     4681 aagtaaatat atatatacac tcttattttt taatatattt aataatttca ctcagaatat
     4741 atatattctt tactcaaatt aaatataact tatatacttt atgtgtaaat tatggtaaaa
     4801 atattattta atcaaatcaa aacttgattt atatacctgg aaaaggtatt atattaaaga
     4861 gtgcttaaaa tacgcatttc ataaatagct atatataaaa taataaataa ggagagtgat
     4921 gggcaatgga tactagtccg ttgcggagag aattttttac attttcatca cttaataata
     4981 tctaaaatca aaactaatac atatattcag ttatataaat tagttaatta atagatacta
     5041 ttaaatgatg agatttatat aaaactgaat aaaggatgtg ttgccatgat aagatattac
     5101 aaaactatag attcaaaatt agagaaactt agtttttttg aagatggctg ttggataaat
     5161 cttgttgaac ctaatcatag tgaaatcaac gaaatttcta atttattaaa tatagatgta
     5221 gaaagcatag aatctgcact agatgaagaa gaacgctcaa gaatagatgt tgaagacaat
     5281 catactctta tacttataga tataccagta gacgaaagtg actctaattc ttctcattat
     5341 actacaatac ccttaggtat tatactaact gaagaagcta ttgtaacagt ttgtgatgca
     5401 caaactaaga ttttaaatga ctttatagtt ggtcatatta aagatttttt tacgtttaaa
     5461 aagactcgct ttttgcttca aattcttcac aaaaatgcag cttattactt gcattactta
     5521 agaaaaataa ataaaatgac aatcataata gagcgagaaa tttacaaatc aatgaaaaac
     5581 aaagaattgg ttcaattatt ggaattagaa aaatctttgg tatacttctc tacctcacta
     5641 aaatctaatg aacttgtttt aaacaaaatg gttagaactg ctggtataaa gaagtatcca
     5701 gatgatgaag acctattaga agatgttata gttgaaaata gacaggctct tgatatggct
     5761 aaaatatatg gagatatatt gagtagaatt atggatgctt tcagtgcaat tataagtaat
     5821 aatcagaata atgttatgca aattttaaca gtagttacac taattttctc aatacctaca
     5881 ataatatcag gattctttgg tatgaatgtc atcaacatgc cattttctaa taacccaaac
     5941 ggattttgga taatacttct tattacctct ataatatgta tagtgattac atttttcatg
     6001 tcaagaaata agctattata aaatattaat aaaaaagcca ttagaattaa ttgatttaca
     6061 tttatcctaa tggcttttat atatttaaat ctatataatc ttataatact ttttaactat
     6121 catgttcttc atctataatc atttcagttt caagcccttc tagcattgaa tctaaacttt
     6181 cttttatatt tagtatataa attggtacat actcttcttc atctattacc tcttcttgca
     6241 acttaaatgg aggaattaat acttcatcaa agtctatatt taaatttgga aagccttcta
     6301 ctatctcttc acaattatcg tactcttcat ttgctaaaga aatgtacata tctaggctct
     6361 ttttataata aagacctgca agcatatact cacacttctt acttccatat ttaatacctt
     6421 ctttataata tttctcagct tctgcgtcat catttaattt taaataaatt tgacctaaat
     6481 tatatattga ttcaacacaa ccttgtgctc tagccttttc ataccaaaat ttagaattta
     6541 cataatcttt atagaacata tcataaatta atcctaccat atgttgagca tacacattat
     6601 tttcattggc aggtgtatca aacatcattt tagactcttc caatttacct tctctaaaat
     6661 aaattctacc tagatgtata cgagcattca tatggttttt gctacttgcc atttcaaaat
     6721 actttctagc attttttgta tttcctaagc tctcatatat acaccccaat ctataactag
     6781 acctcgtatc tccattttca gatgctttct tataccaact tattgcttct tcaatattat
     6841 ttaaatcttc atagatattt cctaatttga tttgacaaga tacattattt ggaatcctcg
     6901 aataatacga aatcgctttt tctatatctt gattctgata atataaatcc cctagatttt
     6961 caagggaact tttgcatcca acagctatag catcctcata atatttaata gcattttcat
     7021 aatctttttc ttcaaaatat atacctgcca gattattttt tgcttctgtt atatcatctt
     7081 cagcagccat tttataataa gtttttgcct tctctatctc attttctcta ttatataaat
     7141 tagcaagtct atacttagaa agtgtatgat ttttttctat tgctttctca taataataaa
     7201 ttgcatctcc cagctcttct tttctatcat atacatatgc aagcctatac tcagacttca
     7261 catcatctct atcagcactc tttttataat acttctctgc atcttctaaa ttatcattta
     7321 tttccataat catacccaag ttaaagcaac ctttcatatg acctgacttg tacaactgtt
     7381 tatataaact tactgttgat ttattatctt tcttttttcc atttaatatt gctaaattat
     7441 attttgcatc atcatctaaa agcacagaac cttttttata gtattcatat gcttcatcat
     7501 ctttctccaa aaaagtatat aataccccta aattaaaaca agctttacct aaattcttgt
     7561 ctgctgctgt ttttaaccat ttttcacacc ttacaaaatc tttcatttta aaatggcata
     7621 cacctaaatt attttgtgct tgtaaataac ctaaatttgc tatcttttca taccaatata
     7681 tagcatattc atagccatct aacttatagt acatattacc taaatcaaaa gcagcatctt
     7741 cacttccact tttatatgcc attgaatacc acttttctgc ttctttaaat tgttttttac
     7801 tttcatatat atttcctaaa gtatattgag attttacgta attatccaat gcagcactct
     7861 tatacaactc tattgcgctt tccatcttac ccatcttttc aaggagggca cccttagtat
     7921 gtacgacttt cgggtcatta gatatatttt ttattttttt tatagaaaat tttgtatttt
     7981 tcattattcg ctacctaccc attttataaa atatattctc tcaaacatta ttatacatta
     8041 taatctacaa ttaatgctat aatattgaat ttttaccttt tgtcactata ttgtaacatt
     8101 aaattttaaa ttttataatc attagatttt attatgtaat tggtatataa tgtatatata
     8161 ggggggacat tttatgaatt tcagcaatat ttttatccaa gttgcagttt tatttataat
     8221 tattttagtt ggatattttg tcagaaaatt caacttatta gatgaccact gcacatctaa
     8281 gctttcaact ttgacaatga cagttttttt accatcaatg ataattagct ccatgcaaat
     8341 taactttgat agtaaaatga tacaaaaaat actattgtta ttatttacat ctttgattat
     8401 gtatatagtt tcaattataa ttgcattttt attgaaatac attttaaaat gtaatgataa
     8461 aaaagatttg ggaatatacc agtatatagt tgttttttca aatgttgctt ttatgggtta
     8521 tcctgtaatt gaagcagttt taggtcatga agcaatattc tatactgcaa tattcaattt
     8581 gccttttaac ttattttctt tcactttagg aatttatctt ttatctaaag gcagtacaag
     8641 tagaggtttt tcaataaaat cacttataag ccctgctaca atagctattg ttattggact
     8701 ttttttattt gtaacaggac ttaggttacc ccaatttata aacgagccac ttgagatgtt
     8761 gggaagtata actacaccaa tatccatgat tatcattgga agcttacttg ctaattcttc
     8821 agctctcgat tgttttgtca acaaaaaatt atatatagtc acttttataa gattattggt
     8881 acttcctgtg attgtgtatt ttattctaaa aggatggata aatgataaga tgattttagc
     8941 tataccagta gtaatttctt caatgcctgc tgctgcaaat actgctataa tggctaatca
     9001 atatgactcc aatataacac tagcttcaca atgtgtgttt tttacaacac tattttcagt
     9061 tatatctata ccttttataa gtattttact cttatcttga tttatttttt tatgtattat
     9121 ataataacat attcaataaa ttacaaatat aaacatttta ttgtagtata ttgatggaat
     9181 tttttcctag atatttgtca ataatatcta tatgtattat agaataggag gcttaaatat
     9241 ggatatggat ttactcttta aaatagtatt tgctatagtt gctataggtg tttctgtaaa
     9301 aatcattaaa actgttgcta gtttagcttt taaaatagcg ttgatattgc taataatttt
     9361 atttgtatat agaatgtttt tgtaaacatt taaaaataag ttttttaaca caatagagaa
     9421 gtaaaataga ttatctttaa tgtaaatttt ttattaaaga cactctattt tacttctcta
     9481 ttagcttatt taaattaaat ttttattata tcttcccata atataaacat cttcaaattt
     9541 tccatctctg attacagcat attttttagt tccctctatt tcaaaaccta gctttttata
     9601 taaatttaat cccttttcat tatccacaat tacttctaat cctactctag ttaacataat
     9661 ccaattatct gctatatcta aaagttgttc cataagcttc ttacctactc ctcttccctg
     9721 ataattctct gcaacactta ttcctatttc tgcgctatgt ctcaatcgtg gagaagaatt
     9781 tacatgtaac ccagcaaaac ctattacatc tttttctcca ttattattag tttcagctac
     9841 aaacatataa tcattttctc ccaaagaagc taacatagct tcagttttat cgactctgtc
     9901 actcattaat gccaaagtat ttctcataac actgtatgtt cttctaattt cattaaaagc
     9961 ttgtgcatct tctcttctta taggtcttat tgttatatct ataggcttac tcatattgga
    10021 ttccccctca aacataatct ttatttttaa ttaaaatttt acatctttat tttatactgt
    10081 tcaaatctat gtatgcttct acctgaggta catatatcat attatctttt atatgtgtac
    10141 ttccaaattc ttttaattct gtaccttctt tatccttagt tattttatat ctaatggatt
    10201 ccataccttc aactacttca gcatgaaaaa acataccttc atattgttta aatctaaaat
    10261 taaaattgtc tttagtaagt tttattatca tatttttatc tactccttat ataattgata
    10321 tttttcttat tattagttgt atatatactg cttattcata ttaatatatt ataacttatc
    10381 atttatttag tataaagtta taaatagata tatttatact tattttacaa tctttcttca
    10441 attgatatat aatatgttta tataaactaa tgtatgtgag gtgaaatatg agcaaaaaaa
    10501 caaaatattt ttctttaatt ctctttccta ttgctttgct tttaaatttt atagccagca
    10561 aagttccaaa tattgttgaa aaatattact cccaattcat agataaaata attgtacaaa
    10621 tattgagtaa agtttctgga attttcccat tttctctcta tgagattaca atgtacataa
    10681 tagttatatc tatcttctta tttttatgtt gtactttatc tacaattttt aataagaaaa
    10741 aaaaattaaa catatattta aaaaattcca ttttaaatat attatccatt gtatcaatag
    10801 tctactttct ttttgtagtg ctttggggaa taaactataa taggattcca ctagaaacca
    10861 cacttataaa taactataat ttaaaaaaca atacctctat ccaatcaaaa caatatagtg
    10921 ttaaagagct tactaaatta tataaattcc ttgtaattaa agcaaatgaa actagaaaac
    10981 tcacattaca agataaaaat ggtattgtaa aatctaatac agattataat ggtgtcataa
    11041 atagagctca actaggatac gataatatct tggatatatt acctggtgtt agtggtagtt
    11101 attctaagcc taagtatatt atttcatcaa atctaatgtg ctatacagga ataactggta
    11161 tatactttcc ttttacaggt gaggcaaatg taaatatagc aatacctgac ttatatattc
    11221 cttgtacagt tgaacatgaa atggcacatc aaaggggatt tgctagtgaa gatgaggcta
    11281 attttatagc gtatcttact tctataaaac accccaatat tgattttaac tattctggat
    11341 atattttagc tttaaattat actgcttctg ctttaagtaa ggttgattat aatgcatatg
    11401 ttgatatttc tgctggcatt tcagattctg tccgtagaga tttaaaaaat gaaagtgaat
    11461 tttggcaaaa atatgaaggt aaaattaatg aaatttctaa tgagtttaac aattcttact
    11521 taaaagctaa tagcgttact gaaggtactc agagttatgg taaaatggtt gatttattgc
    11581 taacttatta tgaattatat ccatataact aaacactaca attcaaaatt ttattcatat
    11641 acagctgata ttaaatcagc tgttttctat ttaaataaat tatttaactt caagcaatct
    11701 tatataattt gatagtactt atactatctg tataacattt caattctaac tattttagtt
    11761 aaaacatttc tattatcttt tttaataaat gttttaacta aaatttttaa aacataaaac
    11821 ttccacatta tttcactatc tatataaaaa tctaaaaatt cacatataaa attacataac
    11881 taaaaaatca taaatttata gaaaacatat attcaaaata aaaaagcatg tatcaaaact
    11941 tgacacatgc ttataatctt atgtatatat atcattttgt cacaataaaa atttacttgt
    12001 aacactgata tttttaaaca aaatagtcct tatttaatta aattaacatt aattaaattc
    12061 gtactcattt tgctatcatt aaacattact tatagtaatt ttctaataca ctatttgaaa
    12121 cttccttccc ttgaaaactt ttcaaccaat tctcatatgc tgagtttttc catacactac
    12181 catcattaaa tgttacttgt tttacacaag atataatgta ctttacacca tgctctccag
    12241 ttgattgttt ccatttatca aacaatagaa aggcatcatt ttgattctta cctggtgcta
    12301 ttgcattttg ctttgcatat tctttctcca tttgtttcca ttctttcaac caattttcta
    12361 cccatgcctt gccttgttgt ttttcaaaca tacttattat atcctgtggt ggtgattgtt
    12421 gcatttttct ataagtatgt gaatatgatt ttggagacac aggagatatg cctgtaacaa
    12481 taccatagtc tacaccagct gggctaaatc caacacttcc tacttcgcca tctgctgcca
    12541 cattttgagc atcccaatat agctcaagtg gttttccatt tttatcatat gctaagcatg
    12601 taatttcata atccataatt gccttttcac tgctatttgt taagcttata acattgctag
    12661 gccaatgata tggagtatca tcatactgta ttttgctatt atttataaat aatggaatat
    12721 tctcattata aacaagacct ttactacttt gaatatctga taccttcttt gtgacagtac
    12781 ttaccgtatt tgctaagtat acatttgttt tttctgaatt ggtcgtacta agagaaaatg
    12841 ttgttgtcgt tccagtaatc agcaaagcaa caactaaaat aggtaagcta gatatttttt
    12901 tatatctcac aattcttcac ctctttttct attaacttat aataaaacaa tttaccaaaa
    12961 cattaaaatc tatgttaaca taatttacca actgttctag taactatatc ctatcatagt
    13021 tactatcaag tttcaatact attgatggaa attttcacta tattaaatct ttttttaatt
    13081 ttataaattt acaatatgat ttaactcata catatatagt caaattagaa tataagtttc
    13141 ttacatttaa tgaagtttat aaaataaaaa atgactagat aattaaatac tgtaaataaa
    13201 aacttattac aaaagtattt aaaaatctag tcaattaata tatatatgaa ttgtatacta
    13261 taatttttca atctcttcta gcaactcatc aactaatttg ttaccatcaa tcattctaac
    13321 tatttcaccc tttttaaata aaagaccttc acctttaccc ccagcaatac ctatatctgc
    13381 ctctctggct tcaccaggtc catttacagc acagcccata attgcaacag tgatattttt
    13441 ttccatacta ccaatctttt cttcaacctc atttacaaca ctaattaagt ctatattaca
    13501 tctaccacat gtagggcatg atataacttt aatcttatca tttaaaagtc ccaaacttct
    13561 taaaatctgt ttaccaacaa taacctcttc aacagggtcg cctgttaaag atattctaac
    13621 agtatctcca attcctttaa gaagaagtgc tcctacccct atagaagact ttattgttcc
    13681 tttgtgaaca cttccagatt ctgtaattcc aatatgaaga ggataatcaa ctttttttga
    13741 tattaattca tatgcatcaa cagttttata aatatcggat gattttagag atataactat
    13801 attatgaaaa tctaagtctt caagaatctt aatatgtccc attgcacttt caactaatgc
    13861 ttcagcagtt gcagagccat acttttttaa aagttctttc tctaaagaac ctccattaac
    13921 tccaattcta atttttaaat ttctttcctt gcatttctca actaccatct taactctctc
    13981 tatacttcca atattaccag gatttattct tattccatcc acaccttgtt ctatagcttc
    14041 aagggctagc ttataatcga aatgtatatc tgcaataatt ggtatattta cactagactt
    14101 tatctttgct atatttttag cagctttaat atcaggaaca gcaactctaa ctatatcaca
    14161 accagcttcc tctaatctct ttatttgagc tattgtagca tctgcatctc ttgtatcagt
    14221 atttgtcata gattgtatac ttataggatt ttctcctcct atttttacag ttcctacact
    14281 tacttctctc gtctttcttc tctcgtaact catattccac ctctatatat tattttatat
    14341 tgaatctctt taaatattaa aatattataa cctacatgta tattataaat taaaaagcta
    14401 tctagttaaa taagaataaa tttcatatat aaaaattaat ttattcttat ttaaaactac
    14461 atagcctatt ttcttaaaat ctctaaaaac taaatcattg aaataatctt agtatatctt
    14521 tatatgtgat aaaaagcata aataacatta aagctccaaa accaacaaca tttatcatgc
    14581 cttctttatt tgggtcgagt tttttaccac ctctaacagc ttctagtaac agcataagta
    14641 ttctccatcc atcaagtgct ggtattggta atagatttac tatacctaaa tttaagctta
    14701 tgacagctgc taaatacact acatttatga tgcctgtacg agcagcatct gataccatac
    14761 caataacacc tactggtcct gctacagcat tgccaatccc tcctggtaca cgtcctgtaa
    14821 tcatttgacc tagaaatgtt agcatctgtt ttgtcatatc tacagtagtt ttacatgcat
    14881 ttactattga ccccaaaaag tctttttctc tttgtggttg aatccctatt tcatactttc
    14941 cattgttatt tttaggtgtt acatttacaa ctttatcact tccatctcta gttatactaa
    15001 gttttaattc tccaccagaa gaagtattta ttatatttgc aacttcatcc caagtcttaa
    15061 cagaattacc attaatttta tttatcttat ctcctacttg aagccctaca gcctgtgcag
    15121 gagtatctgg cattatcttt cctaatttag tcgttggaga acctatatac aaaaatactg
    15181 gaactaaaag tattgctgct aatattatat tgaatattgg tcctgctaca atagtactaa
    15241 atctttgtaa tatagacttg tttccaaaag aatttggact tatttgttct tcatcttcac
    15301 cttccatgct gacatatcca ccaattggaa gtgctcttat agaatactct gtatcttttt
    15361 ttacactata tatcttaggc cccataccta tagaaaactc atttactttg ataccacttc
    15421 tttttgcaaa tataaaatgt cctaactcat gtattaatac aattatacta aatagtatta
    15481 atgcggctat tatagtcata aattcacctt cctatttttt tatcagattt gcaacataag
    15541 ctctactcca tctatctgtt tctaagattt cttctaatgt tggttcactt atgctactat
    15601 gaacttctaa agtcttctct atgtaatatg gtatgtcata gaaaccaatt ttatcctcta
    15661 aaaattcact tactaaaact tcatttgctg aatttaaaac agaggaataa gttcctccca
    15721 tttttaaaca ctcatatgcc aattttaaac atggaaaagt ttctaaatct ggttcttcaa
    15781 aagttaaagt actaaatttt gaaaaattca ttctttcaaa gctactttcc attctatctg
    15841 gatatgtaag agcatactgt attggtagtc tcatatctgg acatcctaat tgagcaatta
    15901 ttgagctatc tgtatactgc accattgaat gaattatgct ttgtgggtga actactacat
    15961 ctatattttc ttgttctact ccaaataacc atctagcttc tatcacttca agtcctttat
    16021 tcataagcgt tgaagaatca atacttattt ttcttcccat actccaattt ggatgcttca
    16081 aagcttcatt tttagttata tttgcaagtt ctcccttttt ctttcctcta aatggaccac
    16141 cagatgctgt aagtattatc ttttctatat tttttttatt ttctccattt agacattgaa
    16201 atatagcact atgttcacta tcaacaggaa gaatatttac attgtatttt tctgcttctt
    16261 tcattacaag ttcaccagca gttaccaacg tttctttatt cgctaatgct atgtctattc
    16321 ccttctttat agcacaaaga gttgggacta atcctatcat acctactaca gcagttaaaa
    16381 ggacatccac ttcatcaagt gatgatatta tttttaatcc ttccattcct gctaaaactt
    16441 ctatttctat atttttaggc aacatttctt ttaatttcaa tgctttattt tcctcaaaaa
    16501 ctgttacata tttaggttta aactctacta tctgttctag caataattca atattactgt
    16561 tagctgatat tgcaactatc tcaaacttat ctctattatc tctaactaca tccaaagttt
    16621 gtttacctat agagcctgta gaacctaaaa ttgatatctt tttcatataa taatcctttc
    16681 taataataaa tataccctgt caaaacatta taacactact aaaacaatta tttttactat
    16741 tcatagaaat ttcatatatt attccacaga ttaaaattaa tctattattt tttgtatttt
    16801 gtggaatatt ataaaatatt ctagtattat taaatgctct tttttatttc cacaacatct
    16861 tctattttac agtctagtgc taaacatatc ttctccattg tatccataga aacagattta
    16921 tcatttccta gtctgcctaa aacagaaata cttatacctg atatatttac aaattccatt
    16981 ttctttattt ttctgtcaat taacaatttc cataatggag cgtatgaaat catgttatca
    17041 cctcgcaaaa tatattctat cacattttat ctattttgga aaattttcat ctatttagat
    17101 aaataattat ttatctaaat agatttcaat tatagtttat taaatataaa tatgttaaaa
    17161 taaaacaaaa gcataagttc aaaaattaaa ctttatatat atttatctca ttattttact
    17221 aaaacataac tatataaaaa atatttacta ttatatatct atttaaaaca cattattata
    17281 gtatcatata aacatatgta aatatataat taaaaaagct aaagattagt aaatttataa
    17341 aaattatatt ttgaataaaa ggagaaagaa tgttgtattt attaaaaatt aatcaatttg
    17401 aaaaaagtta tgatatagga atttttaaaa atgaacaatc aatatataaa tttattgcaa
    17461 aaataccctt tgtaaaaaaa gataatagta attttaatta tacaaattac tatatggaat
    17521 ttgaagatat tcctgaatat tatgaagtaa atttcaataa ttatttatat attattacca
    17581 ggttttcatt cattccagat ggaactgaga tattttttac ttgggaagaa atccattgtt
    17641 gggatgaaaa agttgctacc gagaaaactt ttataaatgg cgaaacatta gtggattcat
    17701 ataactttcc aaataatgaa gtgaaagact acatacaaaa gcgagaatca ttatatatag
    17761 aaacaaaaaa atattatgaa gaaaaaggat taaaaattga gagacatgca ctcggttcag
    17821 aagatggtga atatgttgaa cttacaaatg attctatact ttatttacta gatatagaag
    17881 cagttgaaat aagagaacaa tcaaaaaata tagaagaatt tctatataag tataaaaaac
    17941 gttttaaata atttatattt taaaatacat aacagtacat tgagtcattt aacgagatat
    18001 aaaatgactc aatgtataga ctgataaaaa taattatatt attttttagt tacatatttg
    18061 ttaaaaaatt ctttttcttc tatcggttta gaaaaataaa acccttgtcc tacatcacat
    18121 ttaactgtat ttaaaaattc caagtctttt ttagtttcaa taccttcaca tacaacagac
    18181 atgcctatac gatgtgacat ttcaataata cattgaataa tactatatac tttctcccgg
    18241 ttaaaggttt ctttaagtaa gcttttatct aacttcaatc cattaataga tagagaacat
    18301 aaaagactta atgaagagta tcctgaacca aaatcatcca ttgaaattaa aaatcctaac
    18361 tttttaagtt cctctaaaat tgaaataact ggttgagata tttcattaaa agcactttca
    18421 gttacctcta tctctatata cttaaaagga atttcatatt ctttaacagt atctaaaaac
    18481 ctttgataaa aactattttg gtaaatagta acccttgaaa aattaactgc aatcggatat
    18541 gtatattgat tttttttcat aaacgttttt cttacaaatg aacacgcttt ttttaacata
    18601 taaaagtcca attcaataat aaatccactt ttttcaaaca atggaataaa ttcatctggt
    18661 gataaaaaac caagttctgg agaaaaccat cttacaagag cttctgcgct tacaacattt
    18721 aaagaagata tttgaaattt aggttgcaaa tacatatgaa attcttcatt ttctatcgct
    18781 ttatacatat aattataaat actattttcc atttgtaact gttttaacag attttcatta
    18841 taccatactg tggaggaaat tcctcttgtt ttacttacct tgtgtgcaat atttgcctta
    18901 tccattatag aattgatgga tttataacct aaattatcaa tttcaactaa gtacgctcct
    18961 atacaataag atatagtttg agttacattc atatctaatt cagagattat agcattagtt
    19021 agcatttccc gaatatcctc taaaagagaa tctctatatt ttgcaagtat tacaaaatta
    19081 tctgaaccaa ttcttgcaca agtttctttg atattaaaag ttttagaaag tgcagctgca
    19141 actattatta gaactttatc tccatattca tatccatgtg tatcattaat atatttaaaa
    19201 ttgtcaatat caatatttag cagtacatac tctttgttag atgcattgtg caaaatttca
    19261 atcgctctgt tttcaaatgc aacctgattt tttatttttg ttaaattatc agtataaaat
    19321 attcttttaa accttttttt attataatat tcccaaatta aaagacttat tgttgagaat
    19381 ataagataaa taaatagtgc agtagcaaaa gaatacaatt ttgcattaga gtgtttttca
    19441 gatataaaaa caatttgatt agacaacaca gaatactctt cactcaattt atataattta
    19501 tcacctgaag caccatttct aacttttata atttctttct tcatagattc ccacatagaa
    19561 tccatttgat ttagtttttg ttgaaattct ttattatcac atctttgaaa tccattttct
    19621 ccattacctg ttcttagctt tatcagaata ttatccagtc gttttatcaa gtcatcattt
    19681 ctttcattat tcaattcttg cttaacaacc ttttgagttt cactgcgaat agtaccagtg
    19741 tgacttataa ttttaatatg ctctatactg tgatatagag aaaatataaa aagaataata
    19801 ataactagcc aaatagcaga ataaattaaa aaatcatatt ttttcttcat tattgttacc
    19861 tcatttgtaa tatatataat ttgcaaataa tcaatctctt ataaaaaatg attatcaata
    19921 ttttacagtg atatgtattt atcttaaata gtttaatcat ttatatccta taaattatac
    19981 aaattcatca cctccaacac aaacagcaat atacgttttt ctaaaatttc tttttaaaca
    20041 ttcatcaaca ctatcattaa atcattttta aattgctcaa ttaatttact gcatcacttc
    20101 ttttcttata aaatatcacc ccaataatat atttgtataa tgtctttatg tctcttttta
    20161 aatttggcat atacataatc tatattttaa taaataatta aaatactatt tacctacaat
    20221 ttaactcttt gaatttattt tatcatatt
//
LOCUS       DBGJFU010000072        19879 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.072, whole genome shotgun sequence.
ACCESSION   DBGJFU010000072 DBGJFU010000000
VERSION     DBGJFU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.072"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..261
                     /locus_tag="ACOIAS_002376"
     CDS             <1..261
                     /locus_tag="ACOIAS_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2702083.1"
                     /translation="SDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNE
                     EVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            379..684
                     /locus_tag="ACOIAS_002377"
                     /pseudo
     CDS             379..684
                     /locus_tag="ACOIAS_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            697..1602
                     /gene="truB"
                     /locus_tag="ACOIAS_002378"
     CDS             697..1602
                     /gene="truB"
                     /locus_tag="ACOIAS_002378"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGZ2702084.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            1733..2662
                     /locus_tag="ACOIAS_002379"
     CDS             1733..2662
                     /locus_tag="ACOIAS_002379"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGZ2702085.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            2766..3023
                     /gene="rpsO"
                     /locus_tag="ACOIAS_002380"
     CDS             2766..3023
                     /gene="rpsO"
                     /locus_tag="ACOIAS_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGZ2702086.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3263..4108
                     /locus_tag="ACOIAS_002381"
     CDS             3263..4108
                     /locus_tag="ACOIAS_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGZ2702087.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4233..6344
                     /gene="pnp"
                     /locus_tag="ACOIAS_002382"
     CDS             4233..6344
                     /gene="pnp"
                     /locus_tag="ACOIAS_002382"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGZ2702088.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6610..7353
                     /locus_tag="ACOIAS_002383"
     CDS             6610..7353
                     /locus_tag="ACOIAS_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGZ2702089.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7511..8758
                     /locus_tag="ACOIAS_002384"
     CDS             7511..8758
                     /locus_tag="ACOIAS_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGZ2702090.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGI
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            8793..9038
                     /locus_tag="ACOIAS_002385"
     CDS             8793..9038
                     /locus_tag="ACOIAS_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HGZ2702091.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGDILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9048..10271
                     /gene="dapG"
                     /locus_tag="ACOIAS_002386"
     CDS             9048..10271
                     /gene="dapG"
                     /locus_tag="ACOIAS_002386"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGZ2702092.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10384..11118
                     /locus_tag="ACOIAS_002387"
     CDS             10384..11118
                     /locus_tag="ACOIAS_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HGZ2702093.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11241..13652
                     /locus_tag="ACOIAS_002388"
     CDS             11241..13652
                     /locus_tag="ACOIAS_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGZ2702094.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13655..14713
                     /gene="mnmH"
                     /locus_tag="ACOIAS_002389"
     CDS             13655..14713
                     /gene="mnmH"
                     /locus_tag="ACOIAS_002389"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGZ2702095.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            14707..16041
                     /gene="rimO"
                     /locus_tag="ACOIAS_002390"
     CDS             14707..16041
                     /gene="rimO"
                     /locus_tag="ACOIAS_002390"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGZ2702096.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16028..16570
                     /gene="pgsA"
                     /locus_tag="ACOIAS_002391"
     CDS             16028..16570
                     /gene="pgsA"
                     /locus_tag="ACOIAS_002391"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGZ2702097.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            16858..17904
                     /gene="recA"
                     /locus_tag="ACOIAS_002392"
     CDS             16858..17904
                     /gene="recA"
                     /locus_tag="ACOIAS_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGZ2702098.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGISVSKEIVEE"
     gene            18080..19621
                     /gene="rny"
                     /locus_tag="ACOIAS_002393"
     CDS             18080..19621
                     /gene="rny"
                     /locus_tag="ACOIAS_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HGZ2702099.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8129 a         2012 c         3855 g         5883 t
ORIGIN      
        1 tcagatgtat catggtcaga atttattcgt caattagaat acaaggctaa ttggtatggt
       61 agaaaaattg taaaggtagg taaattcttt gcaagttcac aaatatgcaa taaatgtggg
      121 tataaaaacg aggaagttaa ggatttaaat ataagagaat ggatttgtcc tagttgtaat
      181 gaaactcacg atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta
      241 ataacgattc aaaataaata acttatgtaa gaaccgtagg aactacgggg atagcctgtg
      301 gagaattagt aagacatatt ttaatatgca aaattctgtg aagcaggaac cctgtgactt
      361 tagtcatggg aggttcagtc aaaggtatct agtatagttt taactcaaga catgatgaat
      421 agaaataata ttagctttaa tgatgttgat ggaataacaa catatacaag agacataaaa
      481 ggtatagaag ttggaatttt atttaaagag aaaaaacaaa atgaaattaa agtgagtttt
      541 aggtcaaaaa attatgttga tgtaagtgaa atagccaagt tatttggtgg tggtggtcat
      601 gtaagggctg ctggatgtac tattagagat agtattgata atgcaaagaa aatggtacta
      661 gaggcagtgt taaaatcaat ttaaggtgac agtaaaatga ataagattat aagtatattg
      721 aaacccacag gtatgacatc tcatgatgtt gtaagtagag tgagaaagat tttaaatata
      781 aaaaaagttg gtcatacagg tactcttgac ccagatgctt caggtgtttt accaatctgt
      841 atagggaaag ctacaaaagt atgtgaagtt attttaaaca aagataaatc gtatatatgt
      901 gagcttactt taggtataag tacagatact tatgatgctt caggtgaaat tttaaaaaaa
      961 gttgatgatt ttaaatttag taatgaagac atagaaagag cttttgacac acaaagagga
     1021 gagataaatc aacttcctcc aatttattca gctttaaagg taaatggaaa gagaatgtgt
     1081 gatttagtaa gaagtggtag acaatctgaa attactttaa aaacaagaag agtaaatata
     1141 aaagacataa aaatactaag tataaaaggt aataaagtaa tgttttatgt tgagtgttct
     1201 aaagggactt atgtgagaag catttgccat gatataggag aatatctagg ttgtggagct
     1261 catatgtcgt ttttgaatag aacatcttct ggtaaatttg acttagataa ttcaataaca
     1321 ttagaagaat tagaattatt ttatgaaaat aaaactttag acaaatactt atatgatata
     1381 gattatgtcc ttgattcgtt taattatgtt gtattaaacc ctaatgctat aaaatattat
     1441 agtaatggtg gctcaataga tgataaaagg tttttaaaaa ataatttcga taaagatgat
     1501 gaatttgtaa gagtatacag tacagataat ttcttaggat taggaaaatt atcaaaacac
     1561 aataatacaa taagtgttaa aagtgataag atgttcatat aaccaatgta tttaaaaaaa
     1621 caacaaaaga gatagtacta taataacttc tatagtctat ctcttttact tatgctataa
     1681 tagtttgtga ataagttaag agtaaattat attttagcgg ggaacattta ttatggtgat
     1741 tataaaatct ataaaagaaa tagcaaacat aaaagaaagt gtcattacca taggaaattt
     1801 tgatggtgta cacaaaggac atcaagttct tataggaaaa acagttgaac atgcaaaaaa
     1861 agaaaatata cagagtatcg tatttacatt tgcaaatcat cctgtaaatt actttagacc
     1921 aaactcaaca aaaaatatta taagcaatga tgataaaata aaattattag aagatttggg
     1981 aatagatata gttgtaatca taccatttga tgagtatatg actaaaatac ctgcaaagga
     2041 tttcgtagat gagatactag taaaaaaatt aaaagctaaa aagatagtaa ttggacatga
     2101 ctttacattt gcaagggcaa aagaaggcaa tgttaatcta ttaaaaagct tagaacatga
     2161 atttggtttt gaggtagaag taataaaacc catcaaaatc aatgatgtaa gagtgagtag
     2221 tacttatatt agaagtttag tatcccaggg tgatatggct aatgtcaagg aatatttggg
     2281 tagaaattac aagttagaag gttgtgttat acattctaaa caccttggaa gaactatagg
     2341 attcccaacg gcaaatattg accttaagaa taatatgctc gttccaaaga gaggtattta
     2401 tgcttctatt gtacatattg gagatgagac ttattttggt gctacaaatg taggctataa
     2461 cccaacagtt aatggaaaaa ctttgtctat tgaaacaaat attttagagt ttgatagaga
     2521 tatttatggg gaaaatataa ttgttgaatt tttggaaaga ataagagatg aaagaaagtt
     2581 taattcaata gatgacttaa aaaatcaatt gtataatgat acaaattatg tgtatgaaaa
     2641 ctatgtttgc aaaaacaagt aatgtatgat aaaatgatta aggaaaataa ccattactca
     2701 gcatttgaat actccaatca atgcttagta ataggcgttt ctaaaaaaat acggaggtaa
     2761 tctaaatgat aaacaaatca gaaataataa aagaatatgg aagagctgaa ggggatactg
     2821 gttctccaga agtacaaata gctttattaa cagctagaat aaacgagtta aatggacatt
     2881 taaaaactca taagaaagac caccattcaa gaagaggact attaaaaatg gttggtagaa
     2941 gaagaaactt attagcttac ttaaaggaaa aagatcttga aggatataga gcattaatag
     3001 ctaaattagg attaagaaag taatttcatt ctttataaaa aagcaggtat agtgtatacc
     3061 tgctttttta tagttaattc attataaagt aaaatagtta ataataaaag tagtttatat
     3121 gttataaaaa attataaaat gaataatatt gggtaagaat attaaaatag tatacaaata
     3181 cataatcaat ttaaaaggaa agacaggata aatatagaaa ttttataaca ggataattag
     3241 ttggaataag gaggatatta ttttgaaaat aaagttagta tgcgatagtt tgtgcgatat
     3301 tccagatgaa atatcagaaa aagattatat agagatagtt ccacttacag ttatttttaa
     3361 tgatagggaa tatatagaag gagtagatat aaagaaagaa gagttttata aaaaagtaaa
     3421 agaaatcaag caaataccta aaacttcaca agctacatat atggagttta aagaagtgtt
     3481 tgataagttc ataacagaag gttatcatat aatctgtatg acaggggctg caaacgcatc
     3541 aggaactttt caaagtgcta tgatagctaa aaatgatgta aatgaaaatg aaaaaataca
     3601 tatatttgat actagaaatt tatctctagg tagtggacaa tatgttataa aggcatgtga
     3661 acttcttgaa gaaggactgg gatttgaaga aattatagat gaattagaaa atactcgcag
     3721 tagtgtaaga ttgctttttg caccatatac acttgatttt ttaaagcaga gtggaagagt
     3781 tccagttgca actgctttga taggaaatat gttaaatata aagcctatat ttttctttga
     3841 taatggggaa gctaagttgg taaataaggt tagaggaata aagaatatag caagtaaatt
     3901 ggtcgatata attttagaaa tgaatgaagg gtctttagaa ggaaaaatcg tcacaatagg
     3961 ttgtggagat aatttacacg attgtgaaat attaaaagat gaagtaaata aaagaataaa
     4021 agcaagaaga gtattattta ctcgaggagg agtttctatt tgctctcata cagggccaga
     4081 tatattggct ataagttgtt ctaattagta gtaatataag gtttttgcat atatatgtga
     4141 agataatttt atattgttaa tttatagtat ttttgataat atagtaaata gagtgatata
     4201 cgtgtttttt aataagagga ggacaagcat taatgtttga acataaaata tttaaaatgg
     4261 attttgcagg aagagaactt tctgtagaaa taggaaagat ttgtgaaatg gctagtggta
     4321 gttgtatagt tcgatacagt gatagtatgg taatggtaaa tacaactaaa tcagccaaac
     4381 ctagagacgg aatagacttt tttcctttaa gtgtggatta tgaagaaaaa ttatactctg
     4441 taggtaagat acctggaggt tttttaaaga gagaaggcaa gccttcagag aaagctatac
     4501 ttacatcaag attgatagat agaccaataa gacctttatt cccaaaagga tttagaaatg
     4561 atgttcaggt agttgcaaca gttctatcag tagaccaaga ttgtacacca gatatagtag
     4621 caatgatagg ttcttcaata gccctttcta tatcagacat accatttaat ggaccaacag
     4681 gttcagtatg tgttggcctt gtagatggtg catttgttgt aaatccaaac gcagagcaaa
     4741 gagaaaaaag ttcaatgcat ctagttgttt caggtacaaa ggaagcaata atgatggttg
     4801 aagccggagc tgatgaagtt ccagacgagg taatgcttga tgctatttta tttgctcacc
     4861 aagaaataaa aaaaatagtt gagtttatag aaggtatagt tgcagaagtt ggaaaagaaa
     4921 aaatgcctgt tgaattatac catgcaggag aagaaataac tcaattggtt cgcgaatttg
     4981 caactgataa gatgaaaaaa gcagttcaga cttttgaaaa gttagagaga atggaaaata
     5041 tggatagagt taaagaagaa actttagctc actttgaaga aactttagaa gattttgaag
     5101 attttgttgg agatatagaa gaagtattac aagatataat aaaagaagaa gtaagaaaac
     5161 ttatagtaca tgaaaatgta agaccagata atagaaaact tgaagaaata agacctatat
     5221 ggtgtgaaac tggcatgata cctagagctc atggttcagc catatttact agaggacaaa
     5281 ctcaagtact taatgttgct actttaggtg ctttaggaga tgttcaaaaa ctagatgggt
     5341 tagatgaaga agaaaacaag agatatatgc atcattataa tttccctgca tatagtgttg
     5401 gagaagctag accatcaaga ggaccaggaa gaagagaaat aggacatggt gctttagcag
     5461 aaagagcttt attaccagta ataccatctc aagaagaatt tccttatgct attagattag
     5521 tttctgaagt attaagttca aatggttcta catctcaagc aagtgtatgt ggctcaacat
     5581 tatctttact tgatgcaggt gtacctataa aagatatggt tgcaggtata gcaatgggac
     5641 ttataaagca tgatggtaag gttgcagtgc tttcagatat acaaggtatg gaagaccatt
     5701 taggcgatat ggactttaaa gtagctggaa cagagtatgg tataacagca atacaaatgg
     5761 atataaaaat tgatggtata gataaagaaa ttctacaacg tgctttaaaa caagctaaag
     5821 agggtagaat ccatatacta ggagagatga gaaaaactat atctcagcca aaaccagaac
     5881 tttcaccata tgcacctaag attgttaaaa tgcaaataaa tcctgacaag ataaaagatg
     5941 taataggacc tggtggaaag ataataacta agataataga cgaaactgga gttaagatag
     6001 atattgaaca aactggggaa gtatttatat caggtattga gattgatatg attaagaaag
     6061 ctcaagaact tataaataat atagtagttg aaccagaagt aggaaaaact tataaaggta
     6121 aggtaagtag aataatgaac tttggagcat ttgttgagat acttccagga aaagaaggat
     6181 tacttcatat atctcatata gctcatgaaa gagttgccaa agttgaagat gtattgaata
     6241 taggggatga agttgaagtt aaagttacag agattgatga aaaaggaaga gttaatttat
     6301 caagaaaagt tcttttacca aaaccagaac ataaaaataa ataacttatt atataattaa
     6361 aagcaaagtc ttaggatttt gcttttttaa atacaaaaaa gtagataatt ttaaaatgat
     6421 agttatggat ggcttatagt tctttttctg tcaggcttta ctcttataaa gaagctattt
     6481 aaaagctgat taatagagag ctttattgat atatatagaa tcacttaagt tatatttagt
     6541 gcataaacag agcttatttt taatagtatt aaataagaaa caatttgtac ataattaggg
     6601 ggagttttaa tgataatgat ggtacttaat aagagtaagc tcaaaaaaat cataatacta
     6661 attttagtag tgttattagt attatcagga ggggtaatgg tattaaagtt atttaacaac
     6721 aagccagtat ttaatttatt taatggtgta gatttaccat atgaaagagg tacaaaggat
     6781 aaaagtggat atgttgcatt aacctgtaat atagaccttg gatgggaaac agagtacgta
     6841 gaatccattt tagaaacact taaaaaagaa aatgtaaaga ttacatttaa tgtaactgga
     6901 aaatgggcag aaaagaataa ggatgaatta ttaaagataa aaaaacaagg acatgaaata
     6961 ggtaatcatg gatacaaaca tttagattat tctacattat catatgaaga caattatgag
     7021 caaatagaga cctctaaaaa gataattgaa gaaattatag gtgaaaaaac taaatttttt
     7081 caagcaccag caggttcttt tggtccagaa acagtaaagg ctgctaaagc attagggtat
     7141 acttctatca aatgggatgc cgatactata gattggaaat acaaagacca accagaggta
     7201 atcattgata gaatgaagaa aaaagacatt aaagatagta gtataatttt aatgcatcca
     7261 accaacgcaa cgacaaaatg tatagatgat attatagcta ttgtaagaga aaaaggacta
     7321 aaaccaggaa aacttagtga tgtatttaag tagacaaagt ataaatattt ggttaagatt
     7381 gtataagttg tagttgaatt aattaaaaga ctgacttata atgtaataca tataaaaaat
     7441 aaatataatt aaaacatagt gttttcaata atccaatatg aaacaaagaa taaattgtta
     7501 ggagaatgta atgtataaga caaaaatact ggaaaatggg cttacaataa ttggtgaaga
     7561 aataccatat ctaaagtcaa taacattagg tatttggata aatgcaggct ctaggataga
     7621 agaagcacag gtgagtggaa tttctcattt tatagagcat atgatgttta aaggaaccaa
     7681 aaacagaaca tcaaaagaga ttgctagttc aatagataat ttgggtggtc aaataaatgc
     7741 atttacaagt aaagaatgta catgttatta tgtaaaacta atagatgagc atatagatac
     7801 aggaattgat gtattgagtg atatgatact aaattctaaa tttgataaaa atgatataga
     7861 taaggaaaga cttataatat tggaagaact taagatgtat gaagactctc cagatgactt
     7921 atcttatgat ttattagtgg aaaatatata tgctaatgat ggattaggaa tgaatataat
     7981 tgggacaaaa gaatctctgt acaatataac tagagaatcc atgttagaat acttaaataa
     8041 gtattatatt ccaaataacg ctgttatttc tatagctgga aactttaact ttgatgatat
     8101 ggtggagaaa atcaaatcaa aatttggaca ctgggaaaag aaaaatttaa gtatagacat
     8161 aagtgaggct aagtttaatc cttgctttat atcaaaaaat aaagatactg aacaggtgaa
     8221 cttagctatg tgcctaaaag gtattccttt tgaaaatgat gaagaagtct attcaatggc
     8281 agtggtaaat aatattttcg gtggaagtat aagctcaagg ctttttcaga aaataaggga
     8341 agaaaaaggt ttagtatatt caatatactc atcacagaca ttatatagga aatgtggtga
     8401 attaggtata tttgcaagca tgagtacaga aaatttacaa gatgtatata atttaataaa
     8461 gaaagaaatt gaaaatataa gagaaaacta tcttacagaa aaagaaatat ctgaaagcaa
     8521 ggaacagtta aaaggaaatt atatattgga tttagaaagt actagcagta gaatgatgtc
     8581 tactggtaaa tctatgttac ttagcaaaaa agtaaaaact acagatgaaa tacttgaatg
     8641 tataaataat gttaatatta attcaataaa gaaggttgtt gataaggtat tcaatatcga
     8701 aaatattggt acatgtatag ttggaagaga tgtagaaaag attctacact tggattaata
     8761 ttgaaaaaag taaatctctg gaggtataaa atatgaattt atctgaaata gcaggtaagg
     8821 aaatagttaa cttggtcaca ggagaaagat tgggggtcat tggagaatgt gaccttatac
     8881 tagatgaaac tacaggcgat atattagcgc ttctaattcc aaaagaaaaa ggattttttg
     8941 ccagaagaaa agataagtca tttttagaag taccatggag aaatgtaaaa aaaataggta
     9001 atgacatgat aattatagaa tatgagggat atatttagga ggaaaaaatg agtattttag
     9061 tgcaaaagtt tggtgggaca tcagtagaat catatgaaaa aatgaatgaa gtatgtaaga
     9121 tagtaaaagc atacaaaaaa aatgatgaag aattacaatt agtattagta gtttcagcaa
     9181 tgggaagaaa aggagctcca tatgctacag atacacttat aaatttgtgt agtgcagtaa
     9241 atgatgagcc aagtaaaaga gaacttgatt taataatgtc ttgtggagaa ataatatcag
     9301 gaacaattct tgctaattta ttgaatgcac aggggataga ttctgttttc tttactggtc
     9361 agcaagcagg tataattaca agtgatgaat attctaatgc aaagataaag tatataaatc
     9421 ctaaaaaggt aaaaagagct ttagatgatg gtaaggttgt agttatagct gggttccagg
     9481 gtgtaacaga tgatggagaa ataactactc ttggaagagg cggaagcgat acatcagcag
     9541 ttgctattgg taaagcttta gaatgtgaaa cagttgaaat ttacactgat gtggatggta
     9601 taatgactgc agaccctaga gtagaaccaa acgctaaagt attgagtttt atagactatg
     9661 aggaagtatt ccaaatggct gacaaaggtg caaaagtaat acatccaaga gcagtacagt
     9721 tggctaaaag tggaaatata actttagcca taaagaatac aatgaatcca acttttgaag
     9781 gtacaaaaat aggttcttta tgtagacact tagaagacaa tatagaatat gaacaagaaa
     9841 gagattttaa ggttgctgtt gcaaataaag atagcgtagc acaagttaag attaagtcag
     9901 cagaagaagt gtttacagaa gtattaaatg agatagaaaa gaaacttata actatagata
     9961 tgataaattt ctttatatca gaaaaagcat ttgttgtaga agatgctgat ataaaatcat
    10021 taaaggaaat actagataaa tttgaattag attatgaagt aaatagagac tgtgctaagg
    10081 taacattgat atgttcaaga attgctgatg aaatgtctgg tatcatgtca aaggtagtta
    10141 gaggattatc aaaagcaggg gtatctcttt tacaaacatc agactcaaat atgactatat
    10201 cttgtttagt tagtgaagaa gatatgcata cagcagtaca tgctatacac caacaatttt
    10261 atttaaaata aactgtataa tacaattatt ttttaatcaa atttaagaat ttcatatata
    10321 tcttaatata tttgatttat ttggtaaaac taaattaaat ataattttga taggagtttt
    10381 tttatgaaag attatagaga agataatgaa aaatttgata aagataattg tagtgaagaa
    10441 tctagttgcg aagagagaga atgtataaag caatttggta ccaatgaaat gccacctcaa
    10501 cctcctaaag atattcaatg tataactata attggagaaa ttgagggaca ttttatagga
    10561 aatcctcaaa aaaaagctac aaagtacgag catataattc caatgctata ctcaattgag
    10621 gaaagtaatg atgttaaagg agtgttagtt gttttaaaca ctgttggagg agatattgaa
    10681 gcaggtttag ctattgcaga acttttaaac agtacatcta aaaaagtagt tactcttgtt
    10741 ctaggaggaa gtcatagtat aggtgttcct cttgctacag caggagatta ctcatttata
    10801 gcaccaacag caaccatgat tatacatcca gtgcgaacta ctggtcttgt gattggtata
    10861 aatgagactt ttgaatattt taaaaagatg caagatagaa taattcaatt tataataaga
    10921 acatcaaata tcaaaaaaga tgtacttgaa aaacttatgc atgaaaaaga tgaacttgta
    10981 agtgatgttg gtagtgtatt aataggaaaa gaagctgtgg attatggtct tatagatgaa
    11041 gttggaggac taaaagaagc acttaaaaaa ttaagagagc ttataaaaga atctgaggaa
    11101 gaaaaaaata atgattaaaa tttgtttaaa taataaaatt tatgatataa tgatgtaata
    11161 aagtaggagt gttagtgtta aggcattagc actttttgct gtgaaggggt aaaacttgct
    11221 aaaaataagg agtgatatta atggccacga aaaagaagaa aaaaaagaag gtaaatacct
    11281 taagttttaa tgcagaatat cataatttaa taacaatatt tataggtgta tttttattat
    11341 atagtttaaa ttctaattct atgggatgga tacctgtgtt aatgcaaaat ctttttaagg
    11401 gactatttgg agggctttca atagcaatac catttatagt aattataaca ggacttttag
    11461 gattttttga tggaaatgaa tatatatata ggctaagaaa gactaaacta tactacataa
    11521 ttattttatt tatatttgta ttttatggat tattaaatgc aggaactctt cctgtagata
    11581 gcccactaaa aggtaacatg tttgatgatg ttatgaaatt gggtgtaagt ggacaaggta
    11641 gtggtttgat agcaacaact attgcgtatt atatgagtaa aatatttggt atagcaggtg
    11701 gatggttaat aagtatcttc gcacttatac tttctgtaat gtttatattt aatatttcta
    11761 taaaagattt ggtgtcaaat gcaaaatcaa aatctaaagc atctaaagac agcaatttaa
    11821 cgtttaaaga taaaattgca aatatgaaaa aatctgcaat agatatgatg actgatgaag
    11881 tagatgatac tactataaat aaaaaaccag gtttctttaa agggttaatg tcaaaaggtc
    11941 ttaataatga tgatgaagaa gatgaatatt ttgaagaaga aaatagtgat ggtgtagatg
    12001 ataaaactat aaaaatagtt ggatttaata aggcagaaga tgagtattta gaaatattag
    12061 aaggaactca aagtatgcca gagttagatg ttttaaaaga attacaaaaa gctaccaatg
    12121 aaaatccagt tgtagatact aaaccagaaa aaaaagttga tatagctaaa cctaatttaa
    12181 atatagaaaa aactcaacct atgagtattg tagcagagcc agttaatgag gattattcaa
    12241 actataaaaa gcctagcatt gagcttttaa ataaagtaaa taagaagtct gatgaaaatg
    12301 gtaaaaagaa agtgttgaaa aatgcttctc ttttagaaaa gacactatca gattttggag
    12361 tggaagcaaa aattaatcaa gttacagttg gacctactat aacaagatat gaaatacaac
    12421 caagtccagg agtcaaggta agtaaaattg taaatttaac tgatgatata gctttaagtt
    12481 tagcagctaa aagtataaga attgaagccc caattcctgg taaaagtgca attggaatag
    12541 aagttccaaa tgaagaagct caaatggttg gtgtgagaga agttctagaa agtgaagaat
    12601 ttaataattt taactctcct cttgcaatgg gacttggaaa agacgtagca ggaaaaatta
    12661 taattggaga tataggtaag atgccacact tactaatagc tggttctaca ggttcaggaa
    12721 agagtgtgtg tgtaaataca ttaataagta gtattttata taaggcaaat cctgatgaag
    12781 taaaattact attaatagac ccaaaagtag ttgaacttgc aaattataat ggaataccac
    12841 atctattgat accagtagtt actgacccta aaaaagctgc aaatgcttta aattgggcag
    12901 ttacagagat gaatagaagg tacaaattat ttgcagatgc tcaagtaaaa gatgtaacaa
    12961 gttataatga gaaagcggaa gaaaaacttc ctaaaatagt aataataata gatgagttag
    13021 cagatttaat gatggcaagt gcaaatgatg tagaagatta tatttgtaga ttagctcaaa
    13081 tggcaagagc tgctggaatg cacttgattg tagcaactca aagaccatct gttgacgtta
    13141 taacaggggt aataaaggct aatataccat ctagaatagc atttgcagta tcatctcaaa
    13201 ctgattcaag aacaatcctt gatatgggtg gtgcagagaa gcttctaggt aaaggtgata
    13261 tgttattcta tccacttggt gcagcaaaac cagttagact tcaaggggca ttcatatcag
    13321 agagtgagtc ggaaaaagta atcgattttg taaaaagtca ggttaaagat ggaataaaat
    13381 atgaagaaga tataattgaa actatatcta aggttaatac ttcaaagggt agtgatgagg
    13441 atgagttttt gagtgaagcg atagaatttg tcgtggaaag tggtcaagca tctgcctcta
    13501 tgctacagag aagatttaaa ataggattca atagagcagc tagacttata gattctatgg
    13561 aagaaagagg aataattgga gcaagtgagg gaagtaaacc gagaaaggta ttaataagca
    13621 aacaggattt gcagaattta gaaggtgaat agaattgtca gttataaaaa tagaaaaagc
    13681 acttaagcta gataaagtaa tatttgtaga tgtgagaact gaaggagaat atgaagaaga
    13741 ccatatattg aatgcaatca atatgcctct ttttaaaaat aatgaacata atgaagttgg
    13801 aacaatttat aagatgcagg gaaaacatga agcaatacaa aaaggatttg actatgtatc
    13861 gtataaatta aaggatatat atttacaagc agcagaactt gcattaaatt atgataatat
    13921 tgtcatatat tgtgcaagag gcggaatgag aagtggcagt atagtaaatt tattatcatc
    13981 attaggtgta aatgtatatc aacttgaagg tggatataag gcttatagaa attttgtttt
    14041 agagtattta aggcatgtaa tggatacaaa aaactttgta tctgttcatg gattgactgg
    14101 tacaggaaag actgatttgt tacatttatt agatgaaaaa ggaatagacg tgctggattt
    14161 agaaggcata gctaaaaaca gtggttctac ctttggtttt ataacttttg ataaaaagcc
    14221 accatctcaa aagaactttg aaacaaaact atttgaaagt atattttttt cgaaagagaa
    14281 ttatatattt gtcgagagtg aaagcaaacg agttggaagt gttacagttc caaatgaaat
    14341 tttcgaggca atgacacgag aaggacatca tatattatta gaatgtgata ttgaaaatag
    14401 agttgataga ctttgtagag attatattta tgataaagat gaaggtaata tattgatttt
    14461 aaaagaatgt ataaacaagt ttagaaaaag attgggtaat aaagtagtag atgattatat
    14521 tgatttatta gaatcaggtg aatataaaga acttgtaaaa agatatcttt tagaatacta
    14581 cgacccactt tatatgcatt cagtagagaa atataagtat agtaagatta taaattttaa
    14641 tgatacacaa aaagctttag aagatgttat agacttatat gagagtatat tggaaggaga
    14701 aagtgaatgt taaaaatagc attggaatca ttaggatgct caaaaaattt agtagatgca
    14761 gaaataatga tgggaatttt aaataacaaa ggctataaat taattggaga ttttgaagaa
    14821 gctgatgtaa ttatagtaaa tacatgtggg tttatagagt cagctaaaca agaatctata
    14881 gatacgataa taaactttgc agagttaaaa aaaactggca atttgaagtt gttgatagta
    14941 acagggtgtc tagcccaaag atattctgaa gaattaaaaa ctgaaattcc tgaaatagat
    15001 gctatagtag gaacaggaag ctatcaaaat atagataaaa tacttaaaga attaagtgaa
    15061 atgcatcaaa ttgtaagcct taatgatata gagtttgtat ttaatgaaga tttaccaaga
    15121 tatatatcta cacctagcta tatggcctat ttaaaaattg gagaaggatg ttccaataat
    15181 tgtacttatt gtataatacc aaaactaaga ggtaaatata gaagtagaaa atttgaagat
    15241 ataattaaag aagctaagaa attagctgaa tctggagtaa aagaacttgt ggttatagca
    15301 caggatacga caaagtatgg ttttgattta tatggaaaag aaagattatc agaactttta
    15361 gaagaacttg caaaaataga tggatttaag tggataaggg ttatgtattc atatccagaa
    15421 tctataacag aagaattgat acaagttata aagaaatatg ataatatatg tagttacttt
    15481 gatatgccaa tacagcatgc aagtaacaat attttaaaat taatgaatag aaaaactact
    15541 aaagaagata tattaaataa aatcaatctg ataagaagta atataccaga tgctatactt
    15601 agaactacaa taatagtagg gtttcctggt gaaacagaag atgacttcaa acaacttgtg
    15661 gactttgttg aagaggttaa atttgataga cttggtgcat ttgcatattc aagagaagaa
    15721 gatacaccag cagatagatt gccaaaccat atagatgaag aagtaaaaat acaaagaaga
    15781 gatactttaa tgatgattca acaaaaaata tctgaagaac ttaatgacaa aaagatagga
    15841 aaaacttatg aggtgttaat agaagaacag atagaagata atgtatatac aggaagaaca
    15901 caaggtgatg cagaagaaat tgacagtata gtgtatgtaa aaagtgtaga caatcttgaa
    15961 gtaggagaat ttgtaagtgt tcaaataaat gatgcaatgg aatacgattt aatgggagat
    16021 gttttatatg aacttgccta acaagttaac tttgtttaga atatttttaa taccagtatt
    16081 tgttttaatt atgttgctca atgtaccaaa taaattttta atagcttgta ttatttttat
    16141 aatagcttca attactgatg ctttagatgg aaaaatagct agaaaataca atctagtcac
    16201 tgactttgga aaatttatgg acccattagc agataagtta ttggttattt cagcacttac
    16261 atgtatgata gaagaccatc ttgtaagtag ttggatggta attataatag tggctagaga
    16321 gcttacagtt agcatattaa gagctatagc tgctgctgat ggaaaagtaa ttgctgctgg
    16381 aaacagtgga aaacttaaaa cgataactca aatggtatct atagtgtttt tattgttagg
    16441 tgctcagttt gaaaatgtat tgatattaaa tattggtgaa attctcattc ttatagctac
    16501 actacttaca ttatattctg gatgggaata tctatataaa aataaagagc tttttatgtc
    16561 ttctaagtaa aaaattatgc tttaataaat aaaaactaat gattaataaa taaaaaataa
    16621 caacttaaaa ttttaaaaat gtaaaaaagg gtatactatt atagtatacc tcttttcatt
    16681 ttaagaatat tttcttttgt gtatataatt attaataatg gtttataaat aaaatgtaat
    16741 gaataaaaat ataatcatta gtagcgtttt taaattgact aggtttgatt tatgttatat
    16801 aattaatata taacaagaga acaaatgttt gtagataaat gaagggatgg tatagagatg
    16861 agtgtagatc aagaaaaatt aaaagcgttg aatgaagctt taggtaaaat tgaaaaagat
    16921 tttggtaaag gttcagtaat gaaattggga gaagcaacat ctatgtctat agatgttata
    16981 tcaacaggag cgattggttt agacatagct attggtatag gaggtctacc tagagggaga
    17041 atagttgaag tatatggtcc agaatcttct ggtaaaacta ctgttgcgct tagttgtgta
    17101 gcatcagctc aaaaagatgg aggaatagct gcatttatag atgcagaaca tgcacttgac
    17161 ccagtatatg caaaagcttt gggtgtggat gttgataacc taataatatc tcaaccagat
    17221 acaggtgaac aggctttaga gatagcagag gcattgataa gaagtggagc gatagatata
    17281 atagtaatag actcagtagc agcattagtt ccaaaggctg aaatagatgg agatatgggt
    17341 gattctcatg taggtttaca agctagactt atgtcacaag cacttagaaa gttaactggt
    17401 tcaattaaaa aatcaaattg tgttgctata tttataaacc agttaagaga gaaagtagga
    17461 ataatgtttg gaaacccgga aactactact ggaggacgtg cactaaaatt ctattcatca
    17521 gttagattgg atgttagaaa aatagataca ataaaacaag gtgataaagt tataggtagt
    17581 agaactagag ttaaagttgt taaaaacaaa gtagcaccac catttaagca agctgaattt
    17641 gatataatgt atggagaagg aatttcaaaa attggagacc ttttagatat agctgctgat
    17701 gtagatatag ttaaaaaatc aggttcatgg tatagttaca atgatactaa acttggacaa
    17761 ggaagagaaa atgttaaaaa attcttggaa gataatttag atttaactac tgaaatagat
    17821 gagaaagtta gagcatttta caatttaaat gaagaacatg aagaatcagg tatttcagta
    17881 tcaaaagaaa ttgtagaaga ataagtaaaa atacaaaaaa ataactagta attaaagaca
    17941 tgccacgctc cctattagct tgacacaatg ttgaaaaaat tataaaatta agtgaggata
    18001 aaatatctaa atttaatttt aattattagg aatatttttt aatcatagtg atgacatttt
    18061 gggattatta acgaaggagg tggatgtcat agatagtgta gtaatgattg taataggtgc
    18121 agctgttggt attatagctg gatattttgt tagaaaaaat atatctgaag caaaaattgg
    18181 acaagctgaa aacttggcca aagaaataat agataaagca caccacgact cagaaacagt
    18241 acaaaaagaa aaattactag aagctaaaga agagatccat aagtggagaa cagaagctga
    18301 aagagaaaac agagaaagaa gaacagaagt acaaaagtat gaaaaaagag tagtacaaaa
    18361 agaagaagtc ttagatagaa aattacaaaa tttagaatct aaagaagtaa acctaagtga
    18421 aaaattaaag gtagtggaaa agaaagaaga agaagttgaa gtaataaaaa ctcaacagtt
    18481 agaaaaatta gaaagtatat caggaattac ttctgataag gccaaagaaa tcatcttaac
    18541 caatgcggag agagatgtaa gacgtgaaat gtctataatg ataaaagaga tagaaagtca
    18601 agctaaagaa gaagctgata agaaatcaag agaaattata ggatatgcaa ttcaaaaatg
    18661 tgcagcagat cacgtagccg aaactactgt aacagtagta agcttgccta atgatgaaat
    18721 gaaaggtaga ataataggta gggaaggtag aaatataagg actttagaaa ccttaacagg
    18781 tatagacctt ataatagatg acactccaga agccgtaatt ttatcaggat ttgatccaat
    18841 aagaagagaa attgcaagaa ttgctctaga aaaattaata gcagatggaa gaatacatcc
    18901 agctagaatt gaagaaatgg ttgaaaaggc gagaaaagaa gttgacaata ttataaaaga
    18961 atatggtgaa caagctgcat ttgaaactag tgtacatggg attcatccgg aattaataag
    19021 attattaggt agacttaact atagaactag ttatggacaa aatgtactta aacattctat
    19081 agaagttgct catatagctg gtataatggc tgctgaaata ggagcagata ttagattggc
    19141 taagagggca ggtttattac atgatatagg taaggctgta gaccatgaaa tggaaggtac
    19201 acatgttgaa ataggtatgg atttacttag acgttataaa gagtctaaag aagttataca
    19261 tgccatgagt actcatcatg gagattacga gcctcaaaca gtagaggctg tattagttac
    19321 agcagcagat gcaatatcag ctgctagacc tggtgcgaga agagaaactt tagaagcata
    19381 tataaagaga cttgaaaaat tagaagaaat agctaattca tatgaaggtg tagaaaaatc
    19441 atttgctata caagctggta gagaaataag aataatggta aaaccagaag ctataaatga
    19501 tgaagaaata catctattgg ctagagatat gactaagaaa atagaggatg aacttgaata
    19561 tcctggacaa ataaaagtaa gcataataag agaaacaaga gctatagagt atgcgaagta
    19621 aaagaaataa tatctaaata ataagaaaat atttttagaa atttaaattt gccacaagat
    19681 atgataatat atcttgtggc ttttaaattg tttaagttta attttatata atgcaaaaat
    19741 aatctaagtg ctaaaagtgc tacttacaaa agattaataa aataaagctt tatatatgta
    19801 ttatttatat aattatccaa gtttgtttga tataatgaag ttgtaaaatc aatactttac
    19861 tacaaaataa atagtaaat
//
LOCUS       DBGJFU010000073        19573 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.073, whole genome shotgun sequence.
ACCESSION   DBGJFU010000073 DBGJFU010000000
VERSION     DBGJFU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.073"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            63..218
                     /locus_tag="ACOIAS_002394"
     CDS             63..218
                     /locus_tag="ACOIAS_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702100.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            348..1361
                     /gene="aroF"
                     /locus_tag="ACOIAS_002395"
     CDS             348..1361
                     /gene="aroF"
                     /locus_tag="ACOIAS_002395"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGZ2702101.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1383..2447
                     /gene="aroB"
                     /locus_tag="ACOIAS_002396"
     CDS             1383..2447
                     /gene="aroB"
                     /locus_tag="ACOIAS_002396"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGZ2702102.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2447..3760
                     /gene="aroA"
                     /locus_tag="ACOIAS_002397"
     CDS             2447..3760
                     /gene="aroA"
                     /locus_tag="ACOIAS_002397"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGZ2702103.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3738..4817
                     /gene="aroC"
                     /locus_tag="ACOIAS_002398"
     CDS             3738..4817
                     /gene="aroC"
                     /locus_tag="ACOIAS_002398"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGZ2702104.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4820..6016
                     /gene="pheA"
                     /locus_tag="ACOIAS_002399"
     CDS             4820..6016
                     /gene="pheA"
                     /locus_tag="ACOIAS_002399"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGZ2702105.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6063..6872
                     /gene="aroE"
                     /locus_tag="ACOIAS_002400"
     CDS             6063..6872
                     /gene="aroE"
                     /locus_tag="ACOIAS_002400"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGZ2702106.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6884..7417
                     /locus_tag="ACOIAS_002401"
     CDS             6884..7417
                     /locus_tag="ACOIAS_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HGZ2702107.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDINMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7438..8277
                     /locus_tag="ACOIAS_002402"
     CDS             7438..8277
                     /locus_tag="ACOIAS_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HGZ2702108.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8545..10026
                     /locus_tag="ACOIAS_002403"
     CDS             8545..10026
                     /locus_tag="ACOIAS_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2702109.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVYDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10059..13496
                     /locus_tag="ACOIAS_002404"
     CDS             10059..13496
                     /locus_tag="ACOIAS_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HGZ2702110.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13514..14260
                     /locus_tag="ACOIAS_002405"
     CDS             13514..14260
                     /locus_tag="ACOIAS_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HGZ2702111.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14209..14517)
                     /locus_tag="ACOIAS_002406"
     CDS             complement(14209..14517)
                     /locus_tag="ACOIAS_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HGZ2702112.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            15320..19453
                     /locus_tag="ACOIAS_002407"
     CDS             15320..19453
                     /locus_tag="ACOIAS_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HGZ2702113.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISPPIGST
                     NLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDADNGTF
                     GFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSNVIGA
                     WWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVTGYPP
                     PQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFSEGES
                     AIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTNVFFR
                     DLIPNGLEFIPGTVTVDGVSVPGVDPNTGFSLPDIGGGTGVEVTFDVVVESIPNPNPT
                     NNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEELIYTI
                     KVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETRVIII
                     RGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNSAVPG
                     TLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGTLNSG
                     ISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISIIKVAD
                     EDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWNGSLN
                     IGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSADIEV
                     IKTSNMDTAVPEETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVTFQPW
                     NGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDETDIS
                     PLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYSIDNG
                     VTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNNNTST
                     SEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPASILSP
                     EYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPN
                     PDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTLIDNV
                     SDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNIAEVT
                     STTPDSNLNNNISRVQVEIKQFCCNDCGCKDCCCNCCCNDCGCKNCCCNCCKNDFWC"
BASE COUNT         7956 a         2170 c         3278 g         6169 t
ORIGIN      
        1 ttttctttgt tataattatt aaacaagaag aataataaat tataaaaatg gaggataata
       61 ctatgaaagg aatattaaaa gaactaacag caaaacttta tagctttttt tactttatgt
      121 tctggggctt ttattttagc gctggctatt tcttttacaa aaatatatgt acttcctttc
      181 tggtgaggcc ttattttagt gattttgagc ataagtaaat tttataaaaa tgattatttt
      241 atatatttat ataagattta gtgcttgata gattatttta gagaactcat tagtgagttc
      301 tcttttttat tttaaatata aaattataat attgggaggt agtaaaaatg atagtagtat
      361 taaaaatggg agcagataaa aatgaagtta aaaaattaat tgaggctatt gggagagaag
      421 gagtagaagt taatcccata gatggaacag aattaactgt tttaggttta gttggagata
      481 ctagtaaaat agatgccaaa agaatagaag caaataaggt agttgaaaaa gttatgcatg
      541 tggtagagcc ttttaaaaag gccaatagaa aatttcatcc agaaccttca atcataaatg
      601 ttaatggaat ggaaattggt tcaaagaaaa ttgctatgat agctgggcct tgttctgttg
      661 aaacagaaga ccaaatagta tctatagcta aagatgtgaa aaaatcaggt gcaggatttt
      721 tgagaggtgg agcattcaaa ccaagaacat caccatacgc tttccaagga ttgaaatatg
      781 atggattgga tttattaaaa aaagcaaaag aaaaaacagg gcttcctata gttacagaaa
      841 taatgtcaac acaagatata gatatatttg aagaaaatgt agatgtaatt caagttggag
      901 ctagaaacat gcaaaacttt gatttattaa aagaattagg taaaacaaat aaaacaatac
      961 ttttaaaaag aggattatct gctacaattg aagagtggct aatgtcagca gagtatataa
     1021 tggctggagg aaatgaaaat gtagttcttt gtgaaagagg tataagaact tttgaaacat
     1081 atactagaaa tactttggat ttaagtgcaa ttttagctgt gaagaaactt agtcatttac
     1141 cagttatagt tgacccaagt catgcagcag gtaaatcatg gatggttgac tctttatcta
     1201 aggcagcaat tgcagttgga gcagatggtc taataatcga agttcataat gacccagcac
     1261 atgcactttg tgatggaaaa caatcgatta agccaaatga atatgatgaa ttgataagtg
     1321 aattaaagac tatcgcatca gcagttggta gagaaatata atctataaaa ggatggttaa
     1381 acgtggaaga aaaactatta gtaaatttaa aagaaaatag ctacaatata tttattaaaa
     1441 aaaatatact aaagaatata ggagaagaaa taaaaaaaat atattttgga aataagattt
     1501 ttattgtaac tgatgaaaat atagcaaaat tatacttgga taaagtaaaa aataatttaa
     1561 gtgaatctgg atttgaaatt aaatctttaa tattaaaatc tggtgaagaa acaaaatcat
     1621 ttaatactct tcctaaaata tacgatagtt tacttgactt taagttaact agaaaagatt
     1681 tgattattac tttaggagga ggggtaatag gtgatttagg tggatttgca gcagcaactt
     1741 ttttaagagg tataagtttt attcaaattc caacatcttt attagcacaa gttgatagtt
     1801 ctgtaggtgg aaaagttgga gttgaccttg agagaggcaa aaacttagtg ggaagctttt
     1861 accaaccaaa ggctgtgttt atcgacccaa atgtattatt atcacttcca gaaaaattct
     1921 atagagatgg aatggcagaa gttataaaat atggatgtat aaaagacaaa acttttttca
     1981 ataaactttt aagtttaaaa aatagagaag aagtaatgca acatatagaa tacattatac
     2041 ataaatgttg ctttattaaa aaaactgtag ttgaagctga tgaaaaagac tttggagata
     2101 gaatgcttct aaactttggc catacattag gtcatgctat tgagaaatac tacaatttta
     2161 cgggttatac ccatggagaa gctgttgcca taggaatgta taagataact ttagaaagtg
     2221 aaaagatagg aattacaaaa aaagggacat cagaacttat aaaatcaatt ttaatgaatt
     2281 ataatttgcc ttatgagatt gaattaagag ataataaatc tatagtagat gctatatctc
     2341 ttgataaaaa aaatctaggt gatacattaa aaataatact tttaaaagat attggagaga
     2401 gtattatata taatactact tctaaatttt ttcaggaggt aagataatgg gaagtctaaa
     2461 aatatatcca agtaaattat ctggagatgt aaaaatacca ccatcaaaaa gtatggcaca
     2521 tagggcagtt atttgttcat ctctaagcaa tggaaaaagt agaatttcta atatagattt
     2581 ttctgatgat ataatagcta ctataagagc aatgacatca ttaggagcta ttatagaaaa
     2641 gaaagaagat attttagaaa tttcgggaat tttttctaaa gaaggtattt taaatagaga
     2701 gaatcagttg aatcaaccta aattgacaat agattgtaat gaatcaggct ctacattgag
     2761 atttttagta cctatatctt tagcatttga tggtgtaaaa aggtttatag gtagaggaaa
     2821 tcttggaaaa agaccacttg atacttatta tgagatattt gacagacaga atataaaata
     2881 tagttacaaa gaaaatcagt tagatttgat tatatcaggt aagcttaaac cagatgaatt
     2941 tagagtaaaa ggaaatataa gttctcagtt tatcacaggt ttactgttta tattgcctac
     3001 gctagagagt gattcaaaga taataattac tactgagcta gaatctaaag gatatttaga
     3061 tttaactctt tcaacgataa aagattttgg ggtagaaatt ataaacaata actataaaga
     3121 atttattata aaaggaaatc aaacgtataa agcgagagat tataaagtag aaggagatta
     3181 ttctcaagga gcattttatt tatcagcaga tgctatagga gaagatatta gcatattaga
     3241 ccttaaagaa gattctcttc aaggggatag tgaagtagtt gaaattttat ctaggatggg
     3301 aatggaaata ttaagagaag gcaacaaaat aaaaggaata acaaatggtt taaatggtac
     3361 tttgatagat gcatctcaat gcccagatat aatacctgta ttatctgtag tagcatcctt
     3421 aagtataggg aggactacaa taattaatgc tggtaggctt agaattaaag aatgtgatag
     3481 acttcatgca ataaatgttg agttaaataa acttggagca aatatagagg agaaagagga
     3541 tagtttgata atcgaaggag taagcaagtt aaatggtgga gtagaggtgt ggagtcacaa
     3601 agaccataga attgctatga cacttgcaat tgcttcttgt agatgtgata aaccaataat
     3661 attaaaagat tttgagtgtg tatcaaaatc ttatccacat ttttttaaag attttaagat
     3721 gttaggaggt cgaattgatg agtggaatat ggggaaataa tttaaaagta tcaatttttg
     3781 gagaatctca tggaaatgct ataggtataa atatagatgg tcttccttcg gggattgaac
     3841 ttgaccttga taatattgat aaagaaatga agagaagagc acctggaaaa aattctatat
     3901 ctacttcaag aaatgaaagt gatataccag aaattctaag tggatatttt aatggaagaa
     3961 ctacaggaac gccattatgt gcaatcataa gaaatagtga tactcgttca aaagattatg
     4021 gagagcttaa aaacttaatg agaccaggac atgcagattt cacaggtaat gtgagatatt
     4081 ctggatttaa tgactataga ggtggaggtc atttttcagg taggataaca gctccattag
     4141 tattttgtgg ggctatatgt aaacaaattt tatcacaaaa aggaatagag ataggagcac
     4201 acataaaaaa aattaaaaat atagaagata tgagttttga ttatgtaaat atttcaaaac
     4261 aacagttatc aaatttgcaa actttggaac tacctttact tgatttatca aaggaagaag
     4321 ccatgaaaaa tacgattata gatgcaaaaa atcaaggaga ttcagttggt ggtataattg
     4381 agtgtgcagt agtaggtatt aatgtagggc ttggaaatcc attttttgac tctgttgaat
     4441 ctactttatc acatttattg ttttctgttc ctgctgtaaa aggtgtagag tttggtttag
     4501 gttttgaact tgcagatatg tatggctctc aaagtaatga tgaaatgtat tatgaaggaa
     4561 atcaagttaa gagcaaaaca aataacaatg gaggaattat aggagggatt actactggaa
     4621 tgccaataat tttcaaagtg gcaattaagc caactccatc aattagcaga caacaaaaca
     4681 cagttaatat aaaagataaa aaggacgata ttttatatat taaaggcaga catgaccctt
     4741 gtatagttca aagggctata cctgtaatag aagcagtaac agccattgga atttttgatt
     4801 tgatgaaagg acgataataa tggaggattt aacgagatat agaaatgaga ttgacaatat
     4861 agataaagag cttattcaat tatttgaaaa gagaatgaat acagttcttg aaattgccag
     4921 acataaaatg aaaaataaca ctactatact tcaaaaagat agagaagaaa aagtactaag
     4981 tagagccgta gataatttag ataataaagc atattcacaa gaaacggtac agttttttaa
     5041 ttctataatg gaaattagta gaggattaca aaaaagattt atagataatg atggtgagca
     5101 gaaaatcgag tttagtttag attcatcaaa taagaatttt gatttgtcaa atgtaaataa
     5161 atataagtct ttaaaagatg aattaaataa aaaaaatatc ctagtaggct ttcctggaaa
     5221 atcaggttca tttacagaag aagcactaaa taaattcttc aacaaaaaaa tatcaaaaaa
     5281 acaatttaaa gaatttgaag atgttttcat agcacttaaa aataaagaaa taaactatgg
     5341 aataatcccc atagaaaatt catctacagg agctatctct gaaacttatg accttttgag
     5401 aaagtacggt ttttatatag ttggagaaga atgtataaaa atagaccaaa atttaatagg
     5461 gataaaagac acaaagcttg aagatataaa agaaatatat tctcatccac aaggtatagg
     5521 acaatgtagt gaatttttaa aacaaaatag tacatggaaa ttgataccct ttaataacac
     5581 agcaacaagt gcagaacttg taagaaaact tcaagataaa acaaaagcag caattgcaag
     5641 caaaaaagct gcaagtattt atggattaga aataataagt ccttgtataa atgatatagc
     5701 aaacaactat acaagatttg tagtaatcag taatcaaata cacattgaag aagaatcaaa
     5761 caaaatgagt gtagtatttt ctgttgaaca tgaagcagga aaactttata aagtacttgg
     5821 atactttgct aaaaataata taaatatgac aaaaatagaa tcaagaccta tgaaaaatgc
     5881 ctcatggaga tatttctttt atatagattt tgagtgttct atatacaatg ctaaagtgta
     5941 cgatttatta gaattgatag aacataatac agcatatttt aaatttatgg gagtatataa
     6001 aaataaaact gaatagataa gataaatttt tatttaattt ataattatta ggaggtgtaa
     6061 gtatgaattt tttcggtcta gtaggtgaaa aactctctca cagtgtttca cctcaaatcc
     6121 acaagagagt attcgagata ttgaatattg aaagtgctta taaaaacttt gaaatttcaa
     6181 aagaagatat atctaagcta gacggtgcta taaaattatt aggaatacaa ggggtaaatg
     6241 ttactgtacc atataaggag cgtattatga aatatcttga ttttatatca ccagaggcaa
     6301 aacgtatagg agcagttaat acaattttac tcagagaaaa tatgttatat ggttacaata
     6361 cagattactt tggtcttgat agtatgttta aaatggctaa tattgatgtt caaggaaaag
     6421 tagcagttat attaggaact ggaggtgctt ctaaagctgc tcttacatat tttattgatt
     6481 ctggtataga aaagctttat gtttcaacta gaaaaaagga tgataaaaaa ttattaaata
     6541 gtaaagctat attaatagat tatgaagaac ttaaacatat aaaaggagat ataatattaa
     6601 atgctacacc agtaggtatg taccctaatg taggaatttc accagtgtca aaatctatta
     6661 tacaaaattt tgatatatta atagatttaa tttacaatcc tggagaaaca gagtttttaa
     6721 gaattggtaa tagtatgggt aaaaaaactt gtgatggatt atatatgctg gtggggcaag
     6781 caataaaatc tcaggaaatt tggcaagata ctaaaataga taatagtatt ttagatgtta
     6841 tatataatga actaaaatta gaatttttgt aagggagatt gttatgataa ataaaacaaa
     6901 agaaaaattg atattaatag gtatgccagg tagtggaaaa actacaattg gtaaactttt
     6961 ggccaaagaa tataattgta gtttctgtga tatggatgat tatataattc aaatatctca
     7021 aaaaagtata gcagagttgt tttcagaagg agaagatatc tttaggaact atgagactca
     7081 agcatgtaga gaactttcta taagtgataa aacagtgata tctacaggtg gaggtgtaat
     7141 aaaaaaggat attaatatgg aaatactaaa agaaacaggt ataataattt ttattgatag
     7201 acctatacaa aagattttgg aagatataaa tattaattca agaccattat taaaaaacgg
     7261 aaaagataga ttgtataatc tttacaatga gagaataaat ctttataaaa aattttcaga
     7321 tatagagatt ttaaatgata aatcattaaa taatgctgta tataatatta caaatgcagt
     7381 cagtgaaaat tttaaatttg actttaaaga aaaataatct agggggaaat aaaaataatg
     7441 aatattgtta ttgtaggact aggagttata ggaggctctt ttgctaaagc attaaaaaaa
     7501 gctggatatg aaaatgtatt tggagtagat gtagatttag aaactttaaa aaaagcagaa
     7561 aaagcaaaaa tcataaaaaa aggatgtact acaggtaaag agttatttaa aaaagctgat
     7621 ttaattatat tatctatata tcctagatta gttgtagatt ttctaaataa taataaaaac
     7681 ttttttaaaa aagggactat aattactgat actacaggaa taaaagaaac acttatcaat
     7741 gatgtgttgc aaattattcc agatgatata gattttatat ttggacatcc aatggcaggt
     7801 agggaaaaaa agggtattga ttttgcaagt gaacaagttt ttaatggcgc aaattatata
     7861 attactccaa ctggcagaaa caacataaaa aatttagagt tagttgaaaa tttaatttta
     7921 gagattggat ttaaaagagt aaaaaagtta acttcacaaa aacatgatga gattatagct
     7981 tttacttctc agttacctca tgttatggca gttgctctta taaatagtga tgaagaaggt
     8041 agagatacgg gtaaatttat tggagatagt tatagagatt taactagaat tgcaaatatg
     8101 aatgaagact tatggagtga acttttttta ggaaatagag ataatttact aaaagttata
     8161 gaaaactttg aatcagaagt aaatttagta aaagaagcta tatttaacaa tgataaaaat
     8221 aaattaatag agtattttaa aaaatcatca ataagaagag aagctttaga aaaataaata
     8281 ttaatattta agctatatat agatgggtat aaatataagc gatagtgtat ttaattgtat
     8341 taataaatat ggattcaata taattaaata tgctattgat atatttgtac ccttaactat
     8401 tgttatcttt tagatgataa tgatatttat ttaaatactc tttattaaca atttataaaa
     8461 cttaattgta aaaaatactt attaatagta aaataaagtg aatcaattat attataattg
     8521 ctaaagagag tgaggtgtag ctatttgaaa aaaaatagat taatagcaaa gaatatatca
     8581 atagcattta tagtactatt cttttttagt gtatttacat tcttttatgt aggtaatatt
     8641 aatagagtgt tagaatatga aacaaatgac ataataacag ttacaattgc tggatggatt
     8701 atactttcat ttttattttt aggtattata atttacatat tatattcaaa agcaaatagt
     8761 caaaaaacta tagaaaaagt agcatatacg gattttgtaa caggttattc caactggaga
     8821 aagtttgaat tagatgttac aaatttatta aagaaaacat cacaaaataa taaatatgca
     8881 atggtcatat tcgatattga taaatttaaa gcaattaatg atatatatgg ccataaaaag
     8941 ggaaatttaa tattaaaaga tattgcagat actttaaatg aattgactga tatcaatgaa
     9001 acttttgctc gtgtaagtgc tgataatttc aatatattat taacatataa caaaaaagag
     9061 gatataataa atattatcaa aaagattatg gcaaataatg aacttgtaaa cttatctttt
     9121 ggaatttatg aaatcaaaga taaggattta tctgtaagtg tatatagtga tagagcttca
     9181 cttgcaaaat catcaataaa aaataatagt gatgtaaatt ttgcattttt taatgacaaa
     9241 ttaagagaga aattattatt tgaagataag atagaaaaag aaatggaata tgctcttgaa
     9301 agtgggcaat tcgtaatgta tttacaacca aaatacaata ttaaattaga taaattttgt
     9361 ggaagtgaag ccctagtcag atggcaatat acagaaaaag aggtcatata tccaggtgat
     9421 tttattccta tatttgaaaa aaatggattt attagaaaga ttgatatgta catattagaa
     9481 caagcttgta aagaaatccg gtctttattt gataaaggaa tttcaccttt acctatatct
     9541 gtaaacttct caagagttga tttttttaaa aaagatttta ttgaaaacat agtaaatata
     9601 tgtgatagat ataaaatacc atatagttta atagaaattg aaattactga atctagtatg
     9661 tttggagata ctgatactct atttaatgtt agtagaaacc tacaggatat tggctttata
     9721 gttgcaatgg atgattttgg ctctggatat tcttctgtaa atatgcttaa aaatatacca
     9781 ttaaatgtaa taaaattaga tagaggattt tttgtttatg ataaagatgt tgataagagt
     9841 caaattgtta taaaaagcat agtttctctc attaagcaat taggaataag ggttgttgct
     9901 gaaggaattg aaacaagaag tcaaatagaa atgttaaaaa aagcaaattg tgatattgtt
     9961 caaggatatt atttttcaaa accattacca attaaagaat ttgaaaaatt agtctataaa
    10021 atatagtcag cgagtcttaa atgggaggtg gtgattaagt gataagagca aaatcgttaa
    10081 aaaatgtact gagtattgtt ttaatatttt tatttacaat aatttctttt tctgattttg
    10141 catttaaaac taatgtccag gaagaagaaa atagtaaaat taatttaaga gaaatatccc
    10201 tacaaagttc caatgctata aaaataaaat tagatagttc tctatccttt attaaaagtg
    10261 tggcaaatat ggtttcttat tttaatacta tagataactc aaaaatagtt ccattattag
    10321 ctaatatatc aaaagaatct gactttcaaa gaatctctat tgtatctcct gatggaataa
    10381 gtcataccag tgatggcaaa attatcaatg tgtcaaagta tcagtatttt aagaaagcta
    10441 ttaaaggaga aagtaatata tctgaagttg tgaaatctcc aattgatgga aaagatactt
    10501 ttgttatagc tactcctgta tttaataaga ataacaaaat aattggtgtt ttatacggtt
    10561 cttactacaa tgataaatta gctgaattta tagatataac aagttttaat agtgaagggt
    10621 acatagatat atttgaatct agtggtcaat ttgttttaaa atcttcacat acaaacaaac
    10681 tgattaagtc aagtagtaac atatatgata tatttaaaaa agcttctttt gatgatggtt
    10741 atagctataa aaaaatatat gaagatacaa aaaataacaa gagtggcttt acctcatatg
    10801 aatacaaagg agaaaatcgt tatgctactt atacacccat tggtgtaaat gactggtatg
    10861 taatttctgt gataccacaa tatactgtaa gaagaaacat tgataaaata aatcaattag
    10921 cattttctct tattttcaag gtattcttat tgtttattat gcttataaca tatattattt
    10981 ataaaaacaa taaatctcag aaagatatac taaaagctaa caaagaaatc tcatctatta
    11041 caaatagcat tcctggagga gtacaaaagt gtaccctggg tgaaacatgc aatattaatt
    11101 ttttaagtga tggatttata gagttgtttg gttatacaag agaagaaata aaaacattgt
    11161 ttaataatga tttttatagt ttaatacata aagatgatgt tgaaagaata aaaaaagaaa
    11221 tttttaaaca actttacaaa aataaaactg ttgaattaga atataggatt ataacaaaaa
    11281 atggtaatac agtatgggtt ttagataaag gggagcttat aacagaacct gatggaaaag
    11341 aatatttttg ttgtgtaact gtagatatta ctagaacaaa acaattagaa caagagatgg
    11401 aacaaaaaaa tctagaactt gaaatgatat cagaaaatgt tttaggtggt ataattataa
    11461 cagaatttaa tgatgatttt actataaaat acgctaactc tggttatctt tcaatgattg
    11521 gatatacacg tgaacaatta gaaaatgaac ttaaatacaa agcttgtaat cttgtttttt
    11581 atgaagatag aaagaaggct cttgaaagta tacaacatca attaaacaca aaaggaatag
    11641 ctgtatatca acatagaata agaagaagag atggtaatat tatttgggtt tctataaaag
    11701 gaaggcttat tgttgatgat gatggtattg aaaaaggtat ttggattcta aatgatataa
    11761 cagatttaaa aaatgcagaa caaaaactta aaataaatga agaaagattt agaattgcat
    11821 taaagcaaac atcaaacact atatttgagt atgatataaa aacaaagtca cttttaaata
    11881 ctagtaaaga gcttgataaa tataatattt ataaaattac taaaaatgtt ccatattctc
    11941 tagtagaatt aggagttatt cataaagatt ttagtgatga ttttataaat atgtatgaaa
    12001 atgtaataaa tggagcaaaa aaagcaagtt gtgtagttaa gatgaaactt ccagatgaaa
    12061 attatatatg gaataaagtt accttgacta cgatttttga tgaagatggt aatcctgtaa
    12121 gagcaattgg tatacaggaa gatataagca aacaaaagga agcagagata agatacaatc
    12181 aagaaggtaa gtatcattca gctatattgt cggaagcaat gataacttat gaaataaatg
    12241 tatcaaagga tacttttatc aaaggaaatg aaagttggca aaaaccattt ggaattaagc
    12301 ctgacaataa ctattctcaa atggtaaaaa ctatagcaaa tagtattgta cataaagaca
    12361 ataaagaaac atttttaaat acatttttat gtgaaaactt attggatgca tataaaaata
    12421 atgtgactga aataaaatta gaatatcgaa gaatgaatca agaaaataaa atgatttggg
    12481 tcatttgtac aatgcactta ataaaagatt ctattacgaa cgatataaaa gcacttgctt
    12541 atgttaaaga tattgacaag caaaaaaatg aagaaattag attaaaatta aaagctgaaa
    12601 gagatactct tacaggatta tataataaag gtactacaga aaaattgatt aatgatttct
    12661 tgaactctga ggaagcaagc ttaggaatgc atgcattttt aattgttgat atagataatt
    12721 ttaaatctgt gaatgataat ttaggacatg tatttggaga taaagtatta tttgaagttt
    12781 ctaaaaacct aaaacctata tttagaaaag atgatatagt aggtagaatt ggtggagatg
    12841 aatttatagt attcttaaaa aatattcagt cttttgagaa tatcaaaaag aaagcagaaa
    12901 aaatatgcga tatatttaga aattcttata cagaaaataa aaagacatat aaaatatctg
    12961 gaacagttgg aatttcattt gtaccagaac atggaactgt atttgaagaa ctttataaaa
    13021 aagcagacat agcactttat tatgcaaaga gcaaaggaaa agataagtat gcactttata
    13081 atgattgtat ctaccaaaat gaacttgaaa ataatataag tgataataat ataaagttag
    13141 cagataaatg tgagaaatcc aaaccagtac ttaaagaaat tcttgattct attgataatt
    13201 atatctatat agtgaatcca aatacttatg aacttctttt tgtcaataat aaggtgtctc
    13261 gtttggattt agagataaaa ttaggagaaa cctgttataa atcactatta aaatctgata
    13321 gaccatgtaa taattgccct cttaacggat tagaagaaaa taagcattca aaattcaaaa
    13381 tagagacaaa tacatttggt aaaaatatca gtatagcaag ttggattaat tgcatagatg
    13441 gagaatacaa ctgtcttata gagtgtatag attcttcttt atacaaaaat aaataaaaaa
    13501 ggactatgaa gatatgttaa atagtataac tttgatactt ggtataatat ttacaattta
    13561 ctatttttat ctcaaaatag tttttggagg aatttcattt agtgagattt tcttagttgt
    13621 aggaattgta ctaattatct atcaaatatt taagaaaaaa attaaagaaa agaaggtttt
    13681 ttatagggtg ttaaaaataa taatcagcat ttttttaata gtatttgtag ttactgagag
    13741 tctaattatt ttttatccca aaaatagttt agaatcaaaa agtgattatc tcttaatttt
    13801 aggagcttct gtaaaaaaga ctacaccaag tacaaccctg aagggtaggt tagacactgc
    13861 attgaaatac ctaaaaatta atgataattg ttatgtggta gtatctggtg gtaaaggaag
    13921 tggagaaaaa ataactgagg caaaagctat gaaagattat cttataaaaa atggtataga
    13981 taaaaatagg ataatagaag aggataaatc cacgaatact tatgagaatt ttaaatattc
    14041 aaaattgaag atagaagaac atagtcaaag aaaactaagc gatttaaaag taaaaatagt
    14101 aacaacagat tttcatgttc tcagaagtaa aatattagca tatagaaatg gatataaaaa
    14161 tacgtctttt tatgctagta aatcaaaact ttcatttgta ccaacttact atacaagaga
    14221 attttttgca ttatggaaaa caatcatttt tgatagataa taaataaaaa gactagatat
    14281 aaactcatat ctagtctttt ctattccctt aattctaatc cattttccaa ctttacttgt
    14341 tgtagaaatt tcaatattgg gatttacaag taattgctta taagaagttt tatatttttc
    14401 gattccaaaa tataattttc cttcataatc cattataaat ccaaatggtc ttactcttgg
    14461 tttattatct tcaactgtag caaaatagaa tgttgagttt tcttttaaaa aattcattat
    14521 tttttcatga ttaaatcctc tttgtatctt atactaatat aatgaatata aataaaatat
    14581 tcattatact taatataaat cgatataaaa acaattacaa tattatattg attattcgaa
    14641 attatatata aaattcagaa aaaattttag tttacataac aaaaataaag tcaactaaaa
    14701 ttattgattt tagattaaag taccggatta agcatttaag tagattttaa gcttgatttg
    14761 attggaaagg tacaaactat caccgaaaaa aattaaaccc atttaaaaag caatataaaa
    14821 gcttgttatt tttgagaaaa atagaccctc taaactcaat tagattaaaa ataaaataca
    14881 gaaaattata tctctaaata tcttaaataa ttaagtttaa attttacgat attttgtttg
    14941 attatttata atataaaagc atactcttaa tcttcaaaat ttaaaaagag taaaaaataa
    15001 caaaaaaatc actgacattt cactgacaaa ttgtaatttt gttattataa atacattgaa
    15061 aatacttgat tattgaaaaa tggcataaaa taatacattt tatgccattt actattattt
    15121 gatagttttt aacttatgac ataagatatt ttttaacaca tacaataaaa tataaaaaaa
    15181 ttgatattgc aaataaaaaa aatacatatt agatgttata gaaaaaaagc gcacttatag
    15241 gcttaaaatg tcacataaaa gagttttaaa gaataatata ttatgaacac aaattttgta
    15301 atcacaaagg agtgataata tggcaacagt atcaggacaa atagtttttg atagaaatag
    15361 aagtgctacc attgattctg gagactcagg aatagccaat ataccagttg tattacaaga
    15421 tatagcaact ggagttagac ttgtggttct cacagatgca tcaggaaatt atgtttttac
    15481 taatgttcca aatggtaact atagaattgt agaagctttt ggaactacag gaggagtacc
    15541 aacaccaggg aattttgcaa cagcgatagt aggtccagtt ccaattgcag aaatgccacc
    15601 tattagtcct cctattggtt caacaaactt agatggtaca acacctaaca cactattaat
    15661 aacagtagca ggttcagata taacaaatca aaatatatta aatgggcctg tgatttatac
    15721 acctatagag aatatattgg attcttgcgt atctgtatct aatataaacc ttattacaga
    15781 tgcagataac ggaacattcg gtttttttcc accaggtaca ccaagtaata caggacctga
    15841 aatagaacct tacccaaatg tgacgccaga ttttacgtat gttgtaccag acccaactaa
    15901 gtttacacct ttagatggag aatatacagt acaaaatatt atgacaaatg caatgagtaa
    15961 tgttatagga gcttggtggc gtattgctga ccatactaca ggaaatgaaa caggaagaat
    16021 gatggtggta aatggtttta atcctggagc tgtatttttt aaatcaacag tacaagttac
    16081 acctaacact aattatttat ttagcacttg gattttaaac ctttttaagg taacaggata
    16141 tccaccacca cagctaggtg taagaatatt tgaccaaaat aataatatac tatatcaagc
    16201 aacattagga gctcaaattc cagttaatac aaacgcacca gaatggaaag aagtaggtac
    16261 tgtgattaat tctcaaaata ataatcaact tacagtagaa ttttttagtg aaggagaaag
    16321 cgccattgga aatgattatg ctatagatga tgtttcttta agagaaatag ctgttccaac
    16381 ttttactcca ataaaaacaa gtaacacaga aactgctaat gtaggtgata taatcacata
    16441 tacagttaca ttagataata cttgtgatag ccctttaact aatgtatttt ttagggattt
    16501 aattccaaat ggtttagagt ttatacctgg aactgtaacg gttgatggag tatctgtacc
    16561 aggagtagac cctaatacag gtttctcact tccagatatt ggaggaggaa caggggttga
    16621 agtaactttt gatgttgtgg ttgaaagtat tcctaaccca aatcctacta ataatatagc
    16681 taatatagat tactcctata cacctgtaga aggcggtata ccaaatgatt tttcagtaga
    16741 ttctaatcca gttcctgttg aagtaatatc agcagatatt gaagttacaa aattatctga
    16801 acctactata gtgaatccag gagaggaatt aatatacact ataaaagtag taaataatgg
    16861 accattccca tctgaaaatg tggttttaac agatgatgta ccagcttcta ttataaatcc
    16921 agagtattca ttagatggag gagtaacttt tcaaccatgg actggaagtt taaatatagg
    16981 gacattagaa gtaggagaga caagagttat tataattagg ggtattgtaa atccatcaac
    17041 agtaggaatt ataacaaata cagctgtagt atcttcaact acagcagacc caaaccttaa
    17101 taataataca tctacaatag agacagaagt gaatttatta gcagatattt tagtaatgaa
    17161 aacagcagaa cctaattcag ctgttccagg tactttattg agatatacta ttcaagtaga
    17221 aaacttagga ccagcaaatg ctgaaaatgt aatattaaat gatgatattc cagcttctat
    17281 tataaatcca gaatattcat tggatggagg agcaagtttt caaccatgga atggaagtct
    17341 taatatagga actttaaact ctggaatttc attaacagtt ttaattcaag gaacagttag
    17401 tttaaattct tcagaatata tagtaaatac agcaacagtt tcttcaacta cacctgaccc
    17461 agatttaagc aacaatatat ctaccattat tacaccagtt aacccacaag caggtatttc
    17521 tattataaaa gtagctgacg aggatgtagc tgttccagga gaagaatttg tatatacaat
    17581 tgaaattttt aatgaaggac catctaatgc tacgaatgta gttttaacag atgatatacc
    17641 agatgttatt ttaaatccag aatattcatt ggatggagga gcaactttcc aaccatggaa
    17701 tggaagtctt aatataggta cagttgcacc agggcaatta ataagaatta tcataaaggg
    17761 attagtaagt tcaactgcta caggagatat tactaataca gcagaagtaa gtgcagatgt
    17821 accagaacca gttacggatt catcaacagt gacaactcct atagttccat cagcagatat
    17881 agaagtaata aaaactagca atatggatac agcagttcca gaagaaactt tcagttatac
    17941 tataacagtt acaaatttag gaccatctgc tgcacaaagt atcgttttga cagatgatat
    18001 accagatgtt attttaaatc cagagtattc attggatgga ggagtaactt tccaaccatg
    18061 gaatggaaat cttagtatag gcactttgga tgctggagaa ataagaagta ttataataag
    18121 aggaacagtg agccagacag cagtaggtac aattataaat acagcaacaa taagttcacc
    18181 tacacctgac ccaaatccag ataataatac ttcaacagat gaaacagata taagtccatt
    18241 agcagatatt tctgtgataa aaggcaacga accagttgct ataccaggag gaagatttat
    18301 ttatggaatt gaaattgcaa atgcaggacc atcttttgca gagaacgtaa ctttaacaga
    18361 taatatacca gcttctattt taaatccaga gtactcaata gataatggag tgactttcca
    18421 accatggaat ggaagcctta atataggtac tttagatgct ggagagataa gaaatatcat
    18481 aataagggga acagtaagtc aaacagcaat aggtacaatt ataaatacag caacagtaag
    18541 ttcaactaca cctgacccaa atctaaataa caatacttca acatcagaag cagaggtaag
    18601 ttcatcagca gatatttctg tagtaaaacg aagcaatcag actgttgttg taccaggaga
    18661 tgtactagac tatacaattg aggtgagaaa tgcaggaccg tctactgctc agaacgtaac
    18721 tttaacagat aatataccag cttctatttt aagtccagaa tattcaatag ataatggagt
    18781 gacttttcaa ccatggaatg gaagtcttag tataggcact ttagatgctg gagagataag
    18841 aaatatcata ataaggggaa tagtaagtca aacagcaata ggtacaatta taaatacagc
    18901 aacagtaagt tcaactacac ctgacccaaa tccagataat aatacttcaa catcagaaat
    18961 agatataagt tcatcagcag atatttctgt aataaaatta gcaaataaaa cagaagcatg
    19021 tgtaggagag caaatagatt ttgtaatagt agtatcaaat gcaggaccag aaaatgctca
    19081 aaatgttact ttaatagaca atgtttcaga taagcttaag aaagcagtat tttcacttga
    19141 tagaggtgta agtttccaac catggactgg aagtcttaat ataggtacac taccagctgg
    19201 aactttaaga gtgattatat taagaggaat tataaaatca acatgtcttg atagattaac
    19261 aaatattgca gaagttactt caacaacacc agattcaaac cttaacaata atatatcaag
    19321 agtgcaagta gaaataaaac aattttgttg taatgattgt ggttgtaaag attgttgctg
    19381 taattgttgt tgcaatgatt gtggttgtaa aaattgttgc tgtaattgtt gtaaaaatga
    19441 cttttggtgt tgattttata aaataatttt gtatacctta aattaaactg aaataaaaaa
    19501 taaattcaca tatgagtaaa atattttata aatattaata aattttctat ctttgatatg
    19561 gaccacattc gta
//
LOCUS       DBGJFU010000074        19410 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.074, whole genome shotgun sequence.
ACCESSION   DBGJFU010000074 DBGJFU010000000
VERSION     DBGJFU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.074"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            232..609
                     /locus_tag="ACOIAS_002408"
     CDS             232..609
                     /locus_tag="ACOIAS_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2702114.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            602..1750
                     /locus_tag="ACOIAS_002409"
     CDS             602..1750
                     /locus_tag="ACOIAS_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2702115.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1767..2369
                     /locus_tag="ACOIAS_002410"
     CDS             1767..2369
                     /locus_tag="ACOIAS_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HGZ2702116.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2462..3484)
                     /locus_tag="ACOIAS_002411"
     CDS             complement(2462..3484)
                     /locus_tag="ACOIAS_002411"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HGZ2702117.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3813..3995)
                     /locus_tag="ACOIAS_002412"
     CDS             complement(3813..3995)
                     /locus_tag="ACOIAS_002412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702118.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4308..4727
                     /locus_tag="ACOIAS_002413"
     CDS             4308..4727
                     /locus_tag="ACOIAS_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HGZ2702119.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4858..6699
                     /locus_tag="ACOIAS_002414"
     CDS             4858..6699
                     /locus_tag="ACOIAS_002414"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2702120.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6783..8612
                     /locus_tag="ACOIAS_002415"
     CDS             6783..8612
                     /locus_tag="ACOIAS_002415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2702121.1"
                     /translation="MLTKFYLNLTDLNYILDYHKDLLNTKLEDNSRVYTLYLISLINY
                     IKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLTLSAISIRKYDKVNNFYFSA
                     KKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVEEVSFFKTLKNKALVSRANY
                     IFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFCKCIISLCYLESGNNVEAIK
                     YINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKEAEVILLKTIKLMKVVEGVY
                     KDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYKSFGDVFIYNSKLDYIYKLY
                     GDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANGYELKKDFKLAIEYY
                     KSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKKLENDILKDGLTKLY
                     NRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGHLAGDKVIVKVTDIIRSVFI
                     DITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRVFKLLEFEGIEHIYSKIKNY
                     ITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQYIII"
     gene            8620..10620
                     /locus_tag="ACOIAS_002416"
     CDS             8620..10620
                     /locus_tag="ACOIAS_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2702122.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10882..12141
                     /locus_tag="ACOIAS_002417"
     CDS             10882..12141
                     /locus_tag="ACOIAS_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2702123.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            12202..13989
                     /gene="iorA"
                     /locus_tag="ACOIAS_002418"
     CDS             12202..13989
                     /gene="iorA"
                     /locus_tag="ACOIAS_002418"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HGZ2702124.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            13991..14566
                     /locus_tag="ACOIAS_002419"
     CDS             13991..14566
                     /locus_tag="ACOIAS_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HGZ2702125.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            14619..15689
                     /gene="buk"
                     /locus_tag="ACOIAS_002420"
     CDS             14619..15689
                     /gene="buk"
                     /locus_tag="ACOIAS_002420"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HGZ2702126.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15907..16608
                     /locus_tag="ACOIAS_002421"
     CDS             15907..16608
                     /locus_tag="ACOIAS_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2702127.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            16668..17174
                     /locus_tag="ACOIAS_002422"
     CDS             16668..17174
                     /locus_tag="ACOIAS_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGZ2702128.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            17349..18371
                     /locus_tag="ACOIAS_002423"
     CDS             17349..18371
                     /locus_tag="ACOIAS_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGZ2702129.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(18518..18826)
                     /locus_tag="ACOIAS_002424"
     CDS             complement(18518..18826)
                     /locus_tag="ACOIAS_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HGZ2702130.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(18886..19314)
                     /locus_tag="ACOIAS_002425"
     CDS             complement(18886..19314)
                     /locus_tag="ACOIAS_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702131.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
BASE COUNT         7647 a         2004 c         3308 g         6451 t
ORIGIN      
        1 aatttttatt aaatagtttt aatgtgtaaa taacattcta cactattgta attaaatatg
       61 taaaaagtaa aatatttaaa tagatatatt ttaaaatcta acttttaata ttgtaaaaaa
      121 attagaaatt taacaaaaag tacaaaaatt cacatttttt tcataaagtg tttgactaaa
      181 aaattcacat gctgtagaat taaggtagca ggagttttga gggaggtgta aataatgata
      241 agaaaaatac cacaagcaga actaaaagta atgaaattca tttggaaagt agatgttaca
      301 gtgacatcaa aagatgttat tgaagctatg gaacaaaaat atggctggaa acaaaccacc
      361 acactgacac ttttatctag gctggtaaaa agaggattct tagatgctca aaaaatagac
      421 agatatacac attacacaat aagagtaaaa cacaaagatt atttaagttt tgagacaaag
      481 gatttcctaa gtaatataca tgatgactct ttaagaagct taatttcagc attacatgat
      541 gatgaaaata taggtaaaga taagctagat tttctagaaa gttattttga taatttaaag
      601 gatgaataat tatatgctat atgtttttga aaatcttata aagacaacta ttgtatgtag
      661 tcttggtatt tgtctattat tatttttgaa aagatattta tttaagaagt ttagtaagag
      721 attcaattat tatatttggt tgataatagt ctttagaatg ttactttttc tatttaatta
      781 tacaatagtg tatgaagtaa aagaacccaa agaaaatgca ttaggaaata atattacaca
      841 aattgatatt tctacagata ataatcttat gctgtatgta gcttatttat ggttatttgt
      901 aactatagtc attgcagttt atacatttat aaagtataca agatttaaaa atctagttgt
      961 tgatgtatct tatgatattg aagataatga tataaactgt atctacaaaa acttgttaaa
     1021 agaattaaat ataaaaaaga aaattgaatt aagaggttct gatgaattga taagtccagc
     1081 aggtatggga ttatttaaat cttacatatt cttaccagac tatccttata gtaaagatga
     1141 actaacttgg attttgaaac atgaattaat gcattttaaa aataaagata ttttaataaa
     1201 gtttttagtt ttgtctgtta gaataatata ttggtttaat ccacttgtat atgtaatgag
     1261 caataaagta aatctagatt gtgaattatg ttgtgatgaa agtgttttgg tagaatgttc
     1321 tttaaaagat aaaaaagaat atgcactagc acttataaag tcaattaagt ttagtaaaaa
     1381 ttataatagt aagattttga caaccgagtt tgataagaca aatttggaaa agagacttga
     1441 gagtattgtt aaaaagaaag gcaaaagtgg aatactaatt gctgttttag tatttatgac
     1501 ttctataaca tcttttgttg aagttgatgc tcaagttaga attgatgagg gtaataatgg
     1561 aatgaataac aatattaatg aatttttatt gaataagacg actgataaaa ttataaatat
     1621 ttattataaa gatgcaccaa atgaaattag gaaattttat gaagataaat gcgaattaga
     1681 aggtaaaata cctagagact atgacataat agaaatggat atgaaagatt ataatcaatt
     1741 aataaaataa ttataaggag gattttatgt taacaaaatt aagaaaaata gtagctatta
     1801 tgtgttgctt attgcttact tttacaagtg tggcatttgc tgacacaagt aaagagaaag
     1861 gaggaagtaa agagactgtt ccagagaaaa acctagattc ttttactctt tctgggtata
     1921 tatatacata tgaaaatgcg ccacaagaaa taagagatga acataaacaa aattgtgaag
     1981 agataaatat agaccctaag ccagatgatg agatatttgt tccagaatca gctaatctaa
     2041 tgaatgagga caatagtaaa ggtgtttatg caagttatac tgtttcttat aatataggtg
     2101 ctaaaactat aacaattatg agtaatgagt atttgactat aagtacaaca aaagttatac
     2161 gaaagggtaa tagcggaaaa gaagtaaaag ctgcacaaat catgttaact ttacttggtt
     2221 ataatgtagg tatagatagt tcttttggaa gtaagacata taatgcagtt gtaagtttcc
     2281 aaaaaaaata cggtttgtct gcagatggaa taattggtcc agctacttgg gataaattgg
     2341 gtagactaac tgaccctact ctttcatagg attaaaatct aaaaatatat taaatagtgt
     2401 ctcaatatga tttttcaatt taaatgagac actatttaaa tataaatgtt ataaaactat
     2461 tttacaataa attccatata actctaccat ctatatcctt aacattttga caaccagtca
     2521 aaatcatagt ctcacataac tcattcctaa ccttctctat aaaaaactcc acaccatcaa
     2581 gaccaccgcc aaaagaagca gtaacaaaag gtcttcccat caaaacagca tcagcaccca
     2641 atccaagcat cttaactaca tcaacaccag tcctaactcc accatcaaca agtattgtta
     2701 tctttccctt tacagactta gctatatctg gaagtacctc acaagtacca ggagtataat
     2761 caagtactct tccaccatga tttgaaacaa taatggcact agcaccagac tctacagcca
     2821 tcaaagcatc atctacagtc ataattcctt ttataataaa aggtaatcta gtggattcaa
     2881 ccaattctct aatctcatca attgttttag gagaaaaagg attttcttga aattgattat
     2941 taataagtcc acaagcatca aggtctacac caactgcaaa agcacctgct tcttcagaca
     3001 atctaatttt atctattatc tttgaattat tccaaggctt tataaataca attccatttc
     3061 ccctattatc cttcaataca tctaaattat ccagtaaaaa agtatctaca ttagtatcgc
     3121 caaccatagc ataaattcca gaattagaac aacctctaac aacaggctct atgtattctt
     3181 tttcggaaac tttaccaccc atgttcaata tagtacctga tacaggtgca gcgaatatag
     3241 gagaactcat ttttctacca aataattcta tggatgtatc tggctttgaa acattatgta
     3301 tgactctcat atttatcttt attttttcta gactttttct attttcaatg aaagacgaac
     3361 cacttccttt accacccata ccaggaacat ctccagcaca agctaatcca ttacaaattt
     3421 tacatacttt acatgttcca ttaaagttct ctctagctga ttttagtaag tcattgtaat
     3481 ccatacaaaa tctctccttt ttataatatt aaaattgagt attactatat tgaaaatatc
     3541 ttaattgtaa atatagggat gttattatga tgagctaaac tagataatac atattagata
     3601 ttttttattg ttgtagatag ttaaattata tcatattaga tagtcaaatt ttgaaaaaaa
     3661 ctaacttatt taagtataca ttttgaaaaa attaaaaaag ctatatatag atttttaaag
     3721 aaatctatat atagctttta agtttatgtc aatctttttt gttaatgaat aaataatata
     3781 gaatacaata tacaaaaaag tttcttgaaa tgctaagata atccccattt acaaagcaac
     3841 atcattatac tgtgtaaatt tgaatcaaca atgttttcac catatttcat cataaaatcc
     3901 tctaattgtg tgtcaataaa tgaataatat ccacttatac tatcgatgta tgcagtcatt
     3961 aagtgtatac attcatcact tttatcttta tacatatata tctcccccta tatacaatat
     4021 ataaatttag ataccaaatc gtatcatatt aaatagttaa gtgtgtaaca tctatgcagt
     4081 tatatattta tatcattata ttaataacta aagtgagttt tgacaataga ttttgggaaa
     4141 ctggatgttc aaagtgcaaa cttggatttt aaaatcatta attaagatag cgcataggaa
     4201 tatatgtata cataacttaa ttataaagat tataaacaat tacttttatt aactttattt
     4261 aaatggtaga ataacttgga gattaataat tataaggagg atataatatg agtttatata
     4321 agtttatata tgatgataag gaatatttat taaaagagga gaactgtagt gctttaataa
     4381 atgatgaaga aaatccagtt caaggagtaa gtatttcaaa aataattgat atacttaatg
     4441 aagcagaaga agttgatttt gatgtagaat attatcaaga agcatgtcca ttatgtttag
     4501 aaggagtaaa agaaaagaaa aaattctttc cttttttaga atatcacttt tatattttca
     4561 gtaaagatgg agaatatgta ataagcaata tatctgttga ttataaagga ctatctttta
     4621 ataaactatc aagggcaaat aaagttgata atagttatat tgtaagtgtg ataatttgcg
     4681 aaaattgtca ggattatata gttcagatag aaaattgtat agtttagtgt aaagtatgtc
     4741 ttaatttaag agaaatttct aaattgagac atcaactttt ttcaaaaata gtatggaaaa
     4801 ttactaaatt tatgagataa tgtaagtcgg aggtgagtta caatatatag taaatatttg
     4861 gaagagatag atgatacaat tttagattca atgtttaaac taaccttgaa tgaaagatta
     4921 ttgactaata acgattttat tgaaaataaa attgatgtag taaagaattt aattgattgc
     4981 tataaaactg aacaaaatag tttaatgtta ttaaaatcaa attttttgct agctttatta
     5041 tatggtattc aagggtttag tgaaaaaatg aaagagtata ttttgatttc ctgtaaatat
     5101 attgataaat gcttacccaa agattataaa tttttagcac gattttattc acaaatagct
     5161 attttaagtt taaaaaatga agatgtaaat aaggcaaatt tatatatgga taaatttaat
     5221 cttatttgtg atgaaaataa ttttctaatt gaagaaatta tttttaaatc acaacttatt
     5281 tacattaagg caagtaagtg tatagaatct tctgtaataa ttaaagagat agaaacgttg
     5341 tatataaaga taaaagaaat taacgatttt aattgtaaaa aaatatattt ttttattatt
     5401 ggtaaaatat attttttgtt tttggatgat gctctaattg ctaaggtaaa ttttttaaaa
     5461 gcaagaaaat atgcagattt actaaaggat atgcaagtat cttcactatg taatataaag
     5521 ataggtgagt gtgaatgttc gtttgaaaat tatgaatgtt ccatggaata tttcaatgaa
     5581 gtaataagca ataaaaagta tagaaatgta aatataattc aaaaatatag agcatcaaac
     5641 aacataacta aaatattaat aaagactaga aattatcctg tcgcaataaa taatttagca
     5701 aaatctgaag tatatctaga aaaaattaaa aatcatagct taaaagaagt agagaagttt
     5761 gatttattta taagtttagc catgtattat gcaaaaagta atgaaaaatc ttttgaacaa
     5821 tcaatatgct atttagatag agctaaaaac atattaaata tgataaaaaa tatagaagaa
     5881 tatataatat atgatttaga aataatttat caccaggcaa agatatatta tatctttgga
     5941 tattatgata atgccttaaa cacaaataaa aaactccttg aaaaatctaa aaatgctaaa
     6001 aattataatt atgtgaagtt agcatacaag agtatatatt tgtgttttga aaagattcaa
     6061 aattatgaaa tgagtatgaa atactttaaa atgtattatg agttaaaaat gatgtatata
     6121 aaagctcgaa atagaaatta tatagattcc ttaaattata gacatgagta tatagaaaaa
     6181 gaaataaata acatgagaaa agtaaaactt gatttagata aaaaaaagta tgttgaccat
     6241 ttgacaagtg catataatag aacttttttg aacgattttt tagaaaaaca agaagttagt
     6301 gaatattgca cagcatttat gatagatgta gattacttta aagaatacaa tgatacttat
     6361 ggacactata atggagatat tgctcttaaa aatataacta taataataaa aaaatatcta
     6421 aaaaaagaca tgcttatgat aaggtatggt ggagaagaat ttctgatatt atctgtatgt
     6481 aaagattata aaaaaagtaa aatatttgga agaaaattat gtaggattgt aaagaaattt
     6541 ttaaacaata atttgacagt tagtattggc attgatacat gtaaaaatag tagtatagat
     6601 ataaaagaaa taattgaaaa tgctgataaa gctttatata aagcaaagga aagtggtaga
     6661 aatagatgtt tgcattatca tgattttaag aatttttaac tgttgaaata ggattggtga
     6721 tttacttgaa aaggagttta atagaagatt taaagagaaa tataaacaag atagaatacg
     6781 atatgttgac taaattctat ctaaatttga cagatttaaa ctacatcttg gattatcaca
     6841 aagacctttt aaatacaaaa ttagaagata atagtagagt ctatacttta tatttaatta
     6901 gtcttataaa ctatataaaa aaagatagag aagcatcata caactatttg atagagtcat
     6961 taaagtatat taaaaaattg tcattaaaag atgattcaga tataatatct aaaatttata
     7021 tttatcttac tttatcagct atttctataa gaaaatatga taaggtaaat aatttttatt
     7081 tctcagcaaa aaaaattata agacaaaatc atttaaatga gttgttggta ttgttaaata
     7141 tgagtttatg tgtagaaata agtgctttat atcagaccaa aacggatgta atagctttag
     7201 tagaagaagt gtcttttttt aaaactttaa aaaataaagc tcttgtaagt agggcaaatt
     7261 atatatttgg gagagtttat ctatatttat ttaataactt tataaaaagt atggaatact
     7321 taattgaatg tctaaaattg gctcaagaaa ataatttata ctctatagag gctttttgta
     7381 aatgtataat aagtttgtgt tatttagaga gtggaaataa tgtagaagct ataaaataca
     7441 taaataatgt attagatgtt gcacacagta ataactcaat atcagtaact gaaagagtgt
     7501 ctataaaaat agaccttata tgggcatatt taaaagaaga tatgaataaa gaagcagaag
     7561 ttattttatt aaagactata aaattaatga aagttgttga aggtgtatat aaggattatt
     7621 tatattcatt tatattttta tattcagctg aaattgagtt aaagaaagat aattgtagtt
     7681 ttgaaaaggt aaacacctat ttaattttat caaaaaatat atataaaagt tttggagatg
     7741 tttttatata taattctaaa ctagattaca tatataagtt gtatggagat ttatatataa
     7801 aatttggtaa tatagatgaa ggtataagtt gttatataaa aggtttaaat ttagttaaaa
     7861 agagttcatt gaatcttaga aaaatatcta tattttatgg gctaatagca aatggatatg
     7921 agctaaaaaa agattttaaa ctggcaatag aatattataa aagtatggat tggtattctg
     7981 ataggtggga aaaaaataat tcctataaaa catctcaagc aattcacaaa caatatgaac
     8041 tcagacaaaa acaagagagt ttaagattac tagtggatga taataagaaa ttagaaaatg
     8101 atattttaaa agatggactt actaaactat ataatagaag atatctggaa cagcagttgg
     8161 atttatttaa tgaaaccaga gacaaaaata tggtcgcaat actggtcgat atagattgtt
     8221 ttaaaagtta taatgataat tatggtcatc tagcaggaga taaagttata gttaaggtaa
     8281 ctgatattat aagaagtgta tttatagata ttaccgaaca cataatcaga tatgggggag
     8341 aagaaatttt aatattagta gattctatag aatcaatcgg tgttgaagag attgataata
     8401 tagaattata tattaaaaga gttttcaaac ttcttgaatt tgagggtatt gaacatatat
     8461 attctaaaat aaaaaactat ataactataa gtgctggagt aagtataaaa agatgtcgta
     8521 gtagaaaaga tgttgaaata ttaattgaag atgcagataa gaacctatat aaatcaaaaa
     8581 aagcaggtag aaatcaatac attataattt aaaattgagg tgattttgtt gcgaaaaaaa
     8641 ataggaatta tagcctctga tattgaatta aaagaaagaa tagaagaatt atatagagaa
     8701 gatgtagaaa atggaactat aataatcgac atacttaacc tagacttaat ggaaaatcaa
     8761 ggtcgtatac ttgttgaaaa aggtgcccaa gctataattg gtagaggtgg aggttatagt
     8821 ttagtaatag atacagtaaa tgtacctgtt ataccaatga atatgaagtc aactgacctt
     8881 ttaagagcaa tagaaatagc aaagaaatat tctaaaaaag tagtattgat tcttggagac
     8941 aatgaagttt cttttgacta tgttggctgg agaaatgtca taagtactga aataactgaa
     9001 gaatggtttg aatctaagta tgagataaga agtaaagttg taaaatatat agaccaaaaa
     9061 gatgaaattg taatcgtagg aggaggtttg gcatgttctt ttgcaaggca atatggaatt
     9121 gatagtgtat ttgcaactgc tagtgatgaa tctataagag aagctgtaga atattgtaaa
     9181 aaattattag atacattagg tgaagaaaag tttaataatg aagtgttacg aaatatactt
     9241 gatggtataa aagatggagt aattgctata gatagtaatg gcagtataat tttgtacaat
     9301 gaaagtgcaa aaaacatgct taaagtagag agaaaatgtg cacttaataa atacatctta
     9361 gatgtatttc ctaaaatgga gtggatgtta gattgtttac atgaaaaaga ggacgtagaa
     9421 gatagaaaga taagaaatat aaataatcta attgttaata ctaggacaac attgataaaa
     9481 gttgataata gtacttatgg agttttagga ataatacaag atataaccaa gctacaaaac
     9541 ttagaaagaa aaataagatt tgacttgaat caaaaaggtc tctatgctag atatactttt
     9601 gatgattttt tatttaaaga taaattaact aaagaattta ttgaagaagc taaaaaaatt
     9661 ggaaaaagtg attatacaac tttactttat ggggaaagtg ggtctggtaa agaaattata
     9721 gctcatagta tacacaatat aagtaaaaga aaagataggc cttttgttgc tataaattgt
     9781 gctactatag cagaaaattt acttgaaagt gaactttttg gctatgaaga aggtgcattt
     9841 actggtgcta gaaagggtgg aaaaagaggg ctatttgaac ttgctcatgg agggactctt
     9901 tttttagatg aaataaatag tctatccttt aatattcaga caaaactatt aagagtaata
     9961 gaggaacgtc aaataatgag aattggttct gattatataa tacctctgga tataaggatt
    10021 atagcagcaa cgaacgaatc tttaaccgaa aaaatagtta tgggtacatt tagagcagac
    10081 ttattttata gattgagtag tttagagata aatatacctc ctttaagaga cagaagagaa
    10141 gatataatac ctttgtttaa caattttgta aatgaagtat taaaggatga tggtctcaat
    10201 ggaataaaca gtatagatga aaattttgtt ttgactaaag atgaaatgga taagctatat
    10261 aattatagct ggcctggaaa tgtaagagag ttaaagacaa tagctcaaaa atatgttgtg
    10321 acaggtaaaa ttaaattgag acaagataga gattttaaaa ctaaaaaatt acttcctaac
    10381 tctgaagtag acaaatttaa ttctgaaacg acagcaagtg tagaagtcca agatgaatct
    10441 ataaatataa gtaagataaa tgatggtaag ataagtatag atataaaaga agtaaataag
    10501 tatgttgaag aaaaaataat aagtatgctt tttgctcaag gactttctaa aaatgaagtt
    10561 gcacaagttc ttggaataag caggacatct ttatggaaga aatacaataa aaatatttaa
    10621 atataattac atataaatta ttttgaagta atgttaattt tttaaaacaa gtgttaagaa
    10681 aaattaacat tacttttttg caaattttta tttttttaga caggcatata tatttataag
    10741 aaaaatagtt aaaacactta atttaataat gaaaaaaata ataattctaa acaaaatcaa
    10801 ttttacataa agtgggattt attggcatag taattgctta atatataatg taagagtaaa
    10861 tacaatttta ggggtgatgg tatgagtcaa agtatggttg gtaagcatgc aatgtggcca
    10921 aaagaaaatg atgttatatt tagcatatct ggcagagctc aagcagcaga gaaggctttt
    10981 ggtatggata atgtaataaa tgcaacaata ggcgctctaa tggatgattc aggaaaattg
    11041 ataacaatga aaactgtata tgaagaatat aaagcattag ataactgtga gataggtgca
    11101 tatgcagctc ttgaaggtca accagattac ttagaagcag ttaaaaaagt atttttcaga
    11161 gattacttac cagaaggaca tataagagta ttagcttcac caggaggaag tggtgcaata
    11221 aaattagctg tatggaacta tactaatgaa ggagatgagg tattaacatc tgattggttc
    11281 tggagtccat atgtaagtat agctgaagag gctaatagaa aagtagttaa ttaccaactt
    11341 tttgatgaaa atagaaggtt taattttgag tcgtttaaag aaaaatttgt aaatatagca
    11401 gaaaaacaag gtagagtctt tacaattata aacacaccag cgcataatcc aactggatat
    11461 agtgttgcag atgatgagtg ggataaaata ttagacttat caaaagaagt cgcaaaagat
    11521 aaagacaaaa agataatatt tttcgtagat tcagcatata ttgactttgc aggtgatgat
    11581 gatgtatgta gaaagttttt taagaaattt tctaatttgc ctgaaaacgt tctcgtattg
    11641 gttggattca gtatgtcaaa aggatttaca gcttatggta tgagaatggg ggctataatt
    11701 tgtatatctt caagtgaaga tgtagctgaa gagttccatt actcatgtgt tcattcatgt
    11761 agagcaaatt ggtcaaattg taatagaagt gccatggcag tattatctaa cattgtaaat
    11821 gaccctaaaa aattcaaaga atatgaggat gaaaaagaaa tatacaaaaa catgcttact
    11881 agaagagcag atgtatttgt aaaagaagct gaaagagtag gattagaaat tcttccatat
    11941 atagcaggtt tctttgtaag tataccatgt gataatccaa aagaagtatg tgaagaattg
    12001 actaaacata atttatttgc agttccatta aaaatgggtt taagatttgc agtttgtgca
    12061 gtttctgaag ataaatgtaa gaaagcacca agtattataa aagaagcact tgaaagttta
    12121 gaagtaaaaa taaataatta atttcattga acaaattggg tggactatga atttaataat
    12181 tgtagatgga gggaatttta tatgaaacaa ttgatgacag gtaatgaagc aattgcacgt
    12241 ggagcctatg aggctggtgt taaatatgca tctgcatatc cagggactcc aagtacagaa
    12301 atattagaaa atatagcaac atataaagat gcaatagttg cagagtgggc tccaaacgaa
    12361 aaggtagcac ttgaggctgc tatagggggt tcaattgcag gagcaagaac aatggcttct
    12421 atgaaacatg ttggtttgaa tgttgcagct gatccaatat ttacttatgc atatacaggg
    12481 gtaaatggtg gtatggtact tattacagca gatgagccgg gaatgcattc atctcaaaat
    12541 gagcaagaca ataggatgta tgcaaaattt gcaaagatac ctttatttga gccatcaact
    12601 agtcaagaag ctaaagatat gataaaagaa gcttttgaag ttagtgaaaa atacgataca
    12661 ccagttttat atagagttac aacaagactt tgtcattcaa aaggattagt ggagtgctat
    12721 gatagagaag aagttgaaat taaagagtat gttaaaaatg ctaagaaaat ggtaactgtt
    12781 ccagcaaatg ctcaaataag aaggggcgtt gttgaagaaa gaatggaaat tcttaaaaaa
    12841 ttctcaaatg aaactgattt aaactactat gaaataaatg atacaaaaat aggtgttata
    12901 gcctctggta tgtgctgtaa ttttgccaaa gaagtttttg gaaaaaatgc atcatatatg
    12961 aaactaggat ttacaaatcc attaccttat gaaaaaataa aagaatttgc agaaaaagtt
    13021 gataaaatat atgttataga agaaaatgac ccatttatag aagaacaaat aaaagcatat
    13081 ggaatagatt gtattggaaa agatgtaata ccaccatatg gagaaatgac accagatgtt
    13141 ttaagaaaag ctatatttgg aaaaacaaat gatactatag aatacaaatc tgaattagta
    13201 acaccaagac caccaagttt ctgtgcagga tgtccacata gaggattctt ctatgaactt
    13261 ggaaagagaa aaaatttaat agttggtgga gatataggtt gttatactct tggatttgct
    13321 cctccatata atggaataga ttatgttgtt tgtatgggtt ctgcttttgg aactgcacat
    13381 ggagctcaaa aagttcttaa tatgaaggat gataatgaaa aaagattggt aggtgtactt
    13441 ggagactcaa cattcttcca tacaggaata aatggacttt tagatgttgt ttacaataga
    13501 ggtaattcta tttcggttat attagataat agaataactg gaatgacagg tcaccaagaa
    13561 aacccaggtt caggatatac tttacaaggt gctaaaacta aagaagttga tatagaagga
    13621 ttagttaaag cttgtggaat agaacatgta agagttataa accctaataa tttaaaagaa
    13681 gtaaatgaag cactagattg ggctttagct atagaagatg aaccatcagt aataataaca
    13741 agatggccat gtgtacttaa aaaattctct aaagaagata tagaagaatt taataatcca
    13801 tttaagacta agtgtaaagt tgaccatgat aaatgtatag gatgtaaatt atgtttgaaa
    13861 actggatgtc cagctctatc atttgataaa gagaataagt tatcaaatat agatagaaat
    13921 cagtgtgtag gatgtggagt atgtgcacaa gtttgtccaa aacaagcaat agttaaggag
    13981 gaaaaataat atgactaaga gtatactttt ggtaggtgta ggaggtcaag gaactatact
    14041 tgctagtaaa ttattaacta tgggattgat ggaagctgga tatgatgtaa agatgagtga
    14101 aatacatgga atgagtcaaa gaggaggttc agtttcctca caagttagat atggagattg
    14161 tgttcattct ccagttatag aaattggtgg agcagatata ttagtatctt ttgaaaaaat
    14221 ggaagcttta agatggttca attatttaaa accagaagga aaagctttag taaataatca
    14281 taggatagat tctatgactg ttttaatcgg aggagctgaa tatcaagaaa gtgatataga
    14341 tgcagagtta agtagattaa atgcaaaagt tataaatgca gctgataaag ctgaagaact
    14401 aggaaattca aaaataatga atgttatttt attaggatgt ctagttaaat ctatgcaatt
    14461 agagtctatt gattgggaaa aaattataag tgaaaatgtt aaaccaaaat ttgttgaatt
    14521 aaacataaaa gctttccatg aaggtatgga aatggttagc aaatagttta taatagatat
    14581 aaatagacta tagaaattaa ggtgaaagga agaaaattat gacttacaga atattagcca
    14641 taaatccagg ttctacttct acaaaaatag cagtatatga tggagaagaa caaattcttg
    14701 tgaagacgat agaccatcca gctgaagaga ttgcaaaata taatactata caagaccagt
    14761 ttgaaatgcg taaggaagca gttttgaata ttcttaaaga aaatagtata gacttaaaat
    14821 ctcttagtgc aatagtagga agaggtggag ttttaccacc agtaaaatca ggagcatatt
    14881 tagtaaatga agaaatgatt gatgtactaa gacatagacc agtacttgaa cacgcttcca
    14941 atttaggtgc tgttgtggca catgcaatat cagaacctct tggaatcaac tcatatattt
    15001 atgattctgt tgcagtagat gagcttatag atgtagcgag aatatctgga ctttgtggaa
    15061 tggatagatc aagtgcaggg catgcattaa atactagagc aatggcttta aaatatgcta
    15121 aggataaagg aaaagattat aagagcttaa acttaatagt agctcacatt ggtggaggag
    15181 taagtattta tcttcatgaa aaaggaagaa tggttgatat gctatctgat gatgaaggac
    15241 cattttctcc agaaaggtca ggaagagtac ctgctacaaa attagtggct gcctgttatt
    15301 caggtcaata ttcagaaaga gaaatgacta aaaagataag aggtaaaggt ggtatagttt
    15361 catacctaaa tactgtagat gctagagaag ttgaaaaaat gatagcagaa ggaaatgaag
    15421 aagcaaaaat tatttatgaa gcaatggctt atcagttagc aaaaggtatt ggagagttag
    15481 caactgtagt agatggaaag gtagatgcta taattataac aggtggaatt gcatattctg
    15541 aaatgtttac ttcaatggtt aaaaagaaag ttgagtttat agcaccagta gaaattatgg
    15601 caggagaaaa tgagttggaa tcacttgctt ttggaacttt aagagtacta aatggagaag
    15661 aagaagctag aatttatagt gaaaattaat aagaaaaact aaaaatgttt atatactaaa
    15721 aaagtggtat atccgttttt tatacacgaa agcttagagt ataggtgaaa atgtataaaa
    15781 tttggattgc cactttttat aattaaaatt ttatcttaac ctaataaata attctgtaaa
    15841 atttcgtatt tatatatata ttttattggt atttattgtt attattatta tgaggagtga
    15901 tttttgatgg gaaaaataag tttaaaaagt atgataagat tattttcagg tttttttata
    15961 tttgcaattt cttctgtgct tatgattaat gcacatgtag gtcttatgcc ttgggatgta
    16021 cttcaccaag gtttatctat aaagctaggc ataactatag gtcaggcttc tataatggta
    16081 ggagttgtaa ttgttatatt ggatgcagtt tttggtgaaa atataggatg gggtacttta
    16141 ctcaacatga catttatagg aatttttatt gatttagtaa tattttctgg agtgatacct
    16201 catgcaagca acacttatat aggtgtgttt atggttgtta taggaattat tttggcagct
    16261 atagcaagtt ttctatactt aggtgtttgt ttaggaagtg gaccgagaga tgggcttatg
    16321 attgctcttc agaaaaaaac taataaatct gtacgtcttg ttagaacaat tcttgaaata
    16381 ctagcattag ttgttggatg gttattagga ggttctgtag gtattggtac gcttgtaagt
    16441 gctttaggtt taggatatgt attacaaata gtgtttagaa tatttaaatt tgacacaaaa
    16501 ttgctgaaac atagatttat tatagatgat ataagagaat ggaaggaaaa gaaaagcaat
    16561 gaacataaat gtaaatcaag tattgtaata aaaaatgagc agaattaatt atgctaaaat
    16621 atatttgtag tttatctaat ttatagtaaa gtcaaggaga ttttgaaatg gaattatggg
    16681 atttatataa tgctgatgga attaaaacag gaaatgttat aaaaagaggt aattctattg
    16741 aagaaggtta ttaccatttg gcagttgaag tgtggatttt aaatagtaac tctcaaattt
    16801 taattcagaa aaggtctaaa agtaaaaaaa cattgccaaa tatgtgggga atgacaactg
    16861 gatgtattgt ttctggggaa gacagtttag aaggagcaat tcgcgaagca aaggaagaaa
    16921 tagggataga tataactaaa gatgagatga aagtttttag aagtatgatt catgaagata
    16981 ctctttggga tgtatactta gttaaaaaag aatatgatat atctaaagct attttacagg
    17041 aggaagaagt aagtgatata aagtgggtaa gcacagatga aataagacaa ttactaaaag
    17101 agggtttgtt ttttgaatat ccagagatat acgaattgct atatgagata gataataata
    17161 aattaaatat atgatgtaga gcagatttca gtataagtaa gattatttaa aatgtatctt
    17221 ctctaaattt taatattgtt ttatgaaaat taataagtta tttttagtat ttttctactt
    17281 gttttttgag attaaaataa aaagggcagt cattataaag tcataaaaat ttggggagga
    17341 tttaaactat ggaagataac aaaggattgg cacgtgacag ttcaaaatat aaaattgttt
    17401 ctatatctgt cgtagtaatt attctaatta cattttggta tatgttaaat atggtgcttt
    17461 taacatttat tatgactttt attttctata acttgcttgt agccacaaga aaacgaatta
    17521 agaagttttc ttctttaaat attccagatt cattgataat tatagtgtta tatgcactat
    17581 ttgctatatt gttagtattg attagctatg cagttgtacc aataattata gttcaactta
    17641 cagagttgag tagggttttt agtgattttg atgtaaatca atttgctcaa tctttaggac
    17701 cgaaattgta tcctattgtc tcaaagctgg attttaataa atatatttca caagcagggc
    17761 tacttatagc atcaacagca acaaaagttg gaagttttgg agtaaatata ttattggcat
    17821 ttttactgag tttactcctt ttacttgaaa aaaatgaaat taaaaatttt ggagataaac
    17881 tttctgatag taagatttct tttatttata attcgcttgt attttttgga aagagctttg
    17941 ttaaaaattt tggtgaagta atgaaagtac aagttatgat tgcctttata aattctgttg
    18001 tttctatgat tttcttaggt ttcatgggat tccctcaaat atgggcttta ggatttatga
    18061 tttttgtgtt gggtcttatt ccagtagcag gagttatagt gtctttgatt ccattaactg
    18121 taatagcatt taatacagga ggaataacaa aggtttttgg agttttactt atgatttgta
    18181 tagtgcatgc agtagaaaca tacatattaa accctaagtt gatgtcgaat agaactaaac
    18241 ttccagtttg ttttgttttt attattttac ttgtaggaga gcattattta ggtgtatggg
    18301 gattactaat tggagttcca atatttatgt tccttatgga tattctgggt gttaaattta
    18361 cttctaggta atatattaaa ataataattt atttttatat ataaattatg aattgtttta
    18421 aaaaagaacc tataaataga catcattaat aaagacatct atttgatagg ttcaattttg
    18481 ctttgtataa atactataaa actaaataaa aagaaattta tttcatttca aaagtttcac
    18541 aacttgcatt tctttcattt atatgaactt tttcagcttt gcaaagttca tttttattgt
    18601 atttacattt tacagcttca caatgaatgt tatctgtatc aacttggtta gtttcattag
    18661 aacgactagt tataccagat gtatatttgt cttcaaaaga agcacaataa gtattttctg
    18721 tagtagttgc ttgcattcca tctactttta tactaccagc ataacaatat ccactagtat
    18781 tataagcaca attagtagca gcacaattta aattattatt tttcataaat aacctcctaa
    18841 gttttattta aacttattat gtgaagttat ctaaatttta tacacttatt tacaattaca
    18901 tttatcacat ttaacaacta catctttgga agtttccctt cctaaatttt tattaacatg
    18961 atacacatca acattttcag ttgatccaca tatagaacat ttaaaattgt tttctagtag
    19021 ggttctttct ttaaataact tccaatacat agaattgatg taatcattat aatttacctt
    19081 gaggcatgtc tcaagaaagt ttatgtatag attgtctaaa tctcctgtat ttaattctgt
    19141 atattctggt tcaaaatatt cacaacatcc tctaaaatcg ggctttgtat ctgaaataac
    19201 acatctaata tcatatctat agtctaggct aggcccctta aatgaaagat gtgtgcaaaa
    19261 agaacaagat atttctggta gatttttttc aagttcatgt aatttattat tcataagtat
    19321 tcctccaaaa aatttatttt aagtatataa atgaatagat tattttaaaa cttttattta
    19381 tttatattag agatttagga taaatattcc
//
LOCUS       DBGJFU010000075        19353 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.075, whole genome shotgun sequence.
ACCESSION   DBGJFU010000075 DBGJFU010000000
VERSION     DBGJFU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.075"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(74..1477)
                     /locus_tag="ACOIAS_002426"
     CDS             complement(74..1477)
                     /locus_tag="ACOIAS_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HGZ2702132.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(1939..3834)
                     /locus_tag="ACOIAS_002427"
     CDS             complement(1939..3834)
                     /locus_tag="ACOIAS_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HGZ2702133.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(4461..4604)
                     /gene="scfA"
                     /locus_tag="ACOIAS_002428"
     CDS             complement(4461..4604)
                     /gene="scfA"
                     /locus_tag="ACOIAS_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HGZ2702134.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(4670..5032)
                     /locus_tag="ACOIAS_002429"
     CDS             complement(4670..5032)
                     /locus_tag="ACOIAS_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HGZ2702135.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(5090..5383)
                     /gene="yajC"
                     /locus_tag="ACOIAS_002430"
     CDS             complement(5090..5383)
                     /gene="yajC"
                     /locus_tag="ACOIAS_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGZ2702136.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(5517..6638)
                     /gene="tgt"
                     /locus_tag="ACOIAS_002431"
     CDS             complement(5517..6638)
                     /gene="tgt"
                     /locus_tag="ACOIAS_002431"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGZ2702137.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(6717..7202)
                     /locus_tag="ACOIAS_002432"
     CDS             complement(6717..7202)
                     /locus_tag="ACOIAS_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HGZ2702138.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(7214..8239)
                     /gene="queA"
                     /locus_tag="ACOIAS_002433"
     CDS             complement(7214..8239)
                     /gene="queA"
                     /locus_tag="ACOIAS_002433"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGZ2702139.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(8557..9576)
                     /gene="ruvB"
                     /locus_tag="ACOIAS_002434"
     CDS             complement(8557..9576)
                     /gene="ruvB"
                     /locus_tag="ACOIAS_002434"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGZ2702140.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(9683..10285)
                     /gene="ruvA"
                     /locus_tag="ACOIAS_002435"
     CDS             complement(9683..10285)
                     /gene="ruvA"
                     /locus_tag="ACOIAS_002435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGZ2702141.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(10278..10787)
                     /gene="ruvC"
                     /locus_tag="ACOIAS_002436"
     CDS             complement(10278..10787)
                     /gene="ruvC"
                     /locus_tag="ACOIAS_002436"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGZ2702142.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(11130..11759)
                     /locus_tag="ACOIAS_002437"
     CDS             complement(11130..11759)
                     /locus_tag="ACOIAS_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702143.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            11962..12285
                     /locus_tag="ACOIAS_002438"
     CDS             11962..12285
                     /locus_tag="ACOIAS_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HGZ2702144.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            12553..13407
                     /locus_tag="ACOIAS_002439"
     CDS             12553..13407
                     /locus_tag="ACOIAS_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HGZ2702145.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHTIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(13593..15029)
                     /locus_tag="ACOIAS_002440"
     CDS             complement(13593..15029)
                     /locus_tag="ACOIAS_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HGZ2702146.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSEKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(15100..16482)
                     /locus_tag="ACOIAS_002441"
     CDS             complement(15100..16482)
                     /locus_tag="ACOIAS_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HGZ2702147.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(16618..17526)
                     /gene="garR"
                     /locus_tag="ACOIAS_002442"
                     /pseudo
     CDS             complement(16618..17526)
                     /gene="garR"
                     /locus_tag="ACOIAS_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
     gene            17919..18590
                     /locus_tag="ACOIAS_002443"
     CDS             17919..18590
                     /locus_tag="ACOIAS_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2702148.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            18693..19241
                     /locus_tag="ACOIAS_002444"
     CDS             18693..19241
                     /locus_tag="ACOIAS_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702149.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
BASE COUNT         6174 a         3408 c         2182 g         7589 t
ORIGIN      
        1 ataaatgcta ttttccatta taatttaatt atttatatag cttccttaca agcataattt
       61 tgtgtatatt ttcttattct ttatatcttc cataaaattc atgtgtagca tatagattct
      121 tatctcctgt tatagctata ccaaatccca ttatttcata ccctttatgc aacatattag
      181 ttccttcttc tggatttgtc ttccactcat taaaaataaa gtttcctata tcatctgcat
      241 ctctatttgt atatacaggt ttatctggaa ttttatattt aattatattt tcaccttgaa
      301 ctgctatata tccaggttta atctcactcc aatctaagta tttcaaaaaa gtagaatatg
      361 aattttttcc atttatcgta tgagataaag tcttcttatt tgccattaaa ttagaccaat
      421 catttgctag accctctaat ctgcttgcta cagataacgc tcttactcca ttctcttttc
      481 tatactgctt tgtaagagca tatactttac cctttattct ctctttaaat gctctatcat
      541 ttggattgta atataaacct tgttgatttg gtgaagcttg atttatacat tgcttgataa
      601 cattctgatt tatgcctcct agtacagtca atttgtctgc accacttaaa atactcttgt
      661 tactgccatt ttcaactaaa actacaggag cattttttgc tattgtagag gcagccaaag
      721 cgtctgttaa ttgtaagcct ttacttagat aaaattcttt actattttta taaaactctt
      781 gtataactat cttattagtt tcaaatctat ctgtgccacc caatctaata gaatttgtgc
      841 tcttaactaa ttcgtcgctt attatttcac tagaaccaag cgcataactc tttaagcctg
      901 tcgtgtcaaa tcccacacta tttccatctg taagtattat aggtactcca tctctagcag
      961 ctactggtga tgcacttatc gcatctgctt ctcctgtaaa tccattggct ataattactt
     1021 tatctattgg cttaatttct ttgatttttt cagctatttt tatacttgta tcaaatctat
     1081 tttcaccaaa tattctttgt acaaccttct ttttggatag taactgattc tcaacatttt
     1141 tacttattgt tgattctgaa cctattatat acacagtctt tgctttgttt aatctttcca
     1201 tagttgtgtt tggaatctgg ttttgttttg tcatcaaaat aggcgcattt aaggctccag
     1261 ctaaaccact tgcacttaga ccatctgcta agctattatc tgagttgatt agtattgctg
     1321 tattgtagtt ttgcttatct gctatttttg ctgctgtctc atatctgtca ttccctttta
     1381 tttgctcaac tgatgtcaac gcatttacca taggagtatt taccattact aaaaatagtg
     1441 taagtcctat agctaatatc tttttactat tcttcatatt attttctacc cccaatgtac
     1501 ttttcatatt ttttattagt actcatactt atttttaatc aaaagttaca attttgttac
     1561 catattttat atatgatacc atagttatcc atacaactca aacaatatac ctaaagtttt
     1621 tattctctat aaattcaaaa cattataatt tattttattt aattacgtta taagttcaaa
     1681 ataatttatg ttagtaatat gtttaatcat tataagaagt atctttttat aataattttt
     1741 gcaatatgaa tttctcaaca ttataatcaa aaaataaaat aaggtatctc aatctaattc
     1801 taaatgagac accttatttt tattatttta tcttgttatt tgtatatttt ataatatcta
     1861 cactatgtct tacatattaa cgcattgtat aatcatatat tatctaaata aacttttaat
     1921 attaattcct atatcttatt agttcttagt aaataagttt attttttctg ctattttttc
     1981 aatagcgttt ttagctattc cttcacctat ttgtactaac ttagtagcat tcttaagtct
     2041 taactcaact acttcttctt gtgatttaga aagttcttct gtagttaata ctattggagc
     2101 ctttgaacta gctgcaagtg gtgctgctgt aagtgcatca actaattgtc catttccacc
     2161 tacataacca tcttttgcta catatgcttt agttgcacct gcatagtatt tattgattac
     2221 tttagcatta gtatcttttc tattttctcc agctaatctt tcaactttat tatttaaatc
     2281 tatatcaact aatttgtctt caacttcttt tgatacagaa ttttctccac ccactatttc
     2341 taattctttt ccttttaata atttttcagc atttgcatct aattcgttac ctgtaactat
     2401 tattggagca ccaaattgag cagctctagc tgatatactc atagcatcag cttctccatt
     2461 tccacctact acaaaagcag tcttaaatgt atcttttaat tgttttgcta tttctaaaga
     2521 agtttcaaat ctatcttctc cagaaactct ctcaacttta cttacaccta atttagaaag
     2581 tttttcttca gtttcttttg atactttaga ttctccacct actatatata ttttcttaga
     2641 agatagatta cttccaagta ctcttaatat ttcagcttct atttctgatg gtagtgaatc
     2701 ttttttagat aataatattg gtgcattttt ttgagaggca agtggtgctg atgctaatcc
     2761 atcaactata gcatcttctc caactaaaat tatagcttct gcttctccat ctttaaatct
     2821 ctctttacta acttctactg ctgttgtaaa tcttgaatca ccagataaaa cttctacttt
     2881 taattctttt ccatctttta catcagttaa tactttgtgt aagtctctaa ctaacttatg
     2941 gtttttactt gaaacactta ttgtttctgg cttttcattt gccttgctta aaactatatc
     3001 aaagcttgat ttttctggtt tatctttatc atcactatct tcttgttctt gagtaaggat
     3061 tgcttcattt tcaaatgtta aagcatatcc atttttaact ttattaacta tttctcttcc
     3121 taatgtagtt aatctaattc catcaaataa ttcttctgct gatatgtctt gtaaatctgc
     3181 gtttttaact gttatcgtat gtaattcttt tgaatctatg tcactataac tcttttcata
     3241 accttcaaaa gtaccttctt ctgatgttat cacttttgag aaatctaaat gttggtctcc
     3301 tacatttaat gtaataacat ttgctgaatc ttttgctctt tttacttcaa ctgtatttgt
     3361 agattttata gttgctgtta acttgttatc agaatcttct gctaaagttg cattgtaagc
     3421 attaactgca tctactattt cttgagcagt tttgtagttt tcaattttat agtcaactac
     3481 ttttccacct aacacttgat gccctttatc ttgaatagta acttttaaac tttgcccttc
     3541 tgttaaagag tttattttat tttgtaattg agttgcatta gttaagttta cattgtctac
     3601 ttttatatcg taaactctat catttacatt tgctcctgct tttgtatctt caaacttaac
     3661 ttctaatgct tttctaacct cttcgattag tttagcagaa tctttcgcac ttactgtgta
     3721 atttttatca acattttctg tcacggtatt gtctgcgaat attggtgctg ctgcactcat
     3781 aactgtagca cctgccatta ctattgccat acttctcttt ttgttgctta acatatttat
     3841 gtcctccgaa tattattttt tattttttat attcgtattt cccacacata aataatatta
     3901 tatataatat tatttttcaa tatattttat gtgtttacat aatttactaa cacaaacact
     3961 aataatatct tcttgaaacc cttcccccta cttaggctct ctataattat gctcaaagat
     4021 agaaatccta ttttatagtg attttctact aaacaatcat atactaacaa aagtattcaa
     4081 actccatttt tatatactac tatgcactaa tttttgcttt actcttatta cttaaatata
     4141 tatcactaaa ttaatatcta tctccatata ttaaaatata taacatatta taaaaatcat
     4201 cataaattag agtttaaaat taaaaattat tctaatacat gtaattgata aaaaataaaa
     4261 tataagagta gctactttaa aataaatgct cttacatgat tagatatttt tgcataatta
     4321 aacgataaga aatcaaattt acttctacat aaatagaata tatattttta tataataatt
     4381 gaaaataaaa gaggatacct cataatgata aaagaatcac tttgagatac cctcttacaa
     4441 acaactaaat caataatttt ctatctttca cactcttgat ttgcaactgt acaagaagtt
     4501 ttacaagctg attgacaaga agtttgacac tctccacatc caccttttgc agcactttgc
     4561 tttaaagtag cttgagataa agtttttaca tgtttatttt ccattttcaa cgcctccatt
     4621 tctaattgtt tcctattgat tatatcataa aactacttta atataaactc tatttaaagt
     4681 tgacacccaa tacgcctcca atgcctcctg ctgctgatat tagcaataac ttataaatca
     4741 tccctatttc aaatacaaac ttttcttgtg ctaaaaacac agtcaaaaaa atacatacta
     4801 tgtacaaaag tcctactaaa agcccataca taagtccttt ttctttcatg tgttttgaag
     4861 tgtaaaaccc tcctatagct gcagaaatag tagttataaa tgatgagacc atagttatat
     4921 tatctccacc aatatctgta aatgtcaaaa ataagttata cactaataaa acagccaaag
     4981 ttattatata tgcatatcct agacctttaa aaatataatt agattttccc ataaaaaaac
     5041 ctcctctcaa attaatatat attcgagaaa aggcttttaa attactaatt tagtcttctt
     5101 ctatttcttc ttttttagat ttaacagttc ctatagccca tttttcaaat ggaactttag
     5161 ttctatttgg tcctaattct aatattacaa catcatcttc aactttagct acattagcta
     5221 ctattccacc tattgtaatg accttgtctc ctacacttag gctgcttctc atttctttta
     5281 attgtttgtc ttttttcttt tgaggtctaa tcattaggaa ataaaagatt gcgattacta
     5341 ctacccatag acctatactc ataattattt gttgctgtgg cattaatttt tcactccttt
     5401 gtaaattata ctcttttgta aatcataata aatgtttata taacaattta ctttctctat
     5461 ctttgagtac tattgaatat tatatattaa aatgattata attaatagtg caaaacctat
     5521 atttcatatc catattttgc aaaaaattca tttctaaaat caagtaatct atcttccatt
     5581 atcgcttgtc ttatttgttt cattaaattt aataaaaagt gaagattatg agtagttatt
     5641 aatcttgcac ccagtatttc atttgctttt ataaggtgtc ttatataagc tcttgaatag
     5701 tttttacaag cataacaatc acattcaggg tcaagtggag taaagtcttc agcataagtt
     5761 gcatttctaa ctactacttt accttggcta gtcattgcag ttccatttct agcgatacga
     5821 gtaggtagaa cacaatcaaa catgtctact cctcttataa caccttctac taagtcatct
     5881 ggactaccaa ctcccattag gtatcttggt ttatcctttg gcataagtgg agttgtatgc
     5941 tctaacacgt catacataag tggttttggt tctccaacac taagtccacc aatagcatat
     6001 cctggtaaat caatattagt tatttctttc gctgattgtt cccttaagtc tttatacatc
     6061 ccaccttgta ttataccaaa taaagcttgc ttgtcggtat tattatgggc atctttacat
     6121 ctttttaacc atcttgttgt tctttctaaa gaatttttta catattctct atctgatggg
     6181 tatggtgcac actcatcaaa tgccatcatt atgtctgaac ccaatgcatt ttgtatttcc
     6241 atagcttttt ctggagttag aaaatgtttt gaaccatcta agtgtgacct aaattctaca
     6301 ccttcttctt ttatctttct taaaggccct agactaaata cctggaaccc tccactatct
     6361 gtaagtattg gtttatccca attcataaac ttatgaagac caccagctct ttttataagt
     6421 tcatgacctg gtctcatata taaatggtat gtgttactaa gtattatttg tgaaccaatc
     6481 tcttttaatt cttctggtgt catagatttt acagttgctt gagttccaac tggcatgaat
     6541 attggtgttt caataatccc atgtggagtg tgtaatcttc ctaaccttgc accactctgc
     6601 ttacaagtct ttatcaattc gtatcttact gcgtacataa agttctcctt aatcatattt
     6661 atttctcatt atttcttaag atatatttgt aataagctat tcatataatt tattgtctaa
     6721 atagatttat cttcgtcttc tttgttctgt tgttccttaa ttgcttcttc atcatgaata
     6781 ctcattatat taagagcact cttgtctttt gttactaaaa acttttttat agcaaatagt
     6841 cctgctatag ctataactcc tattaaaaca tctatcatac cttcattttt aggcagtagc
     6901 aacatttttc tagcaattgc gtaaagtaat gcctcaataa atgctcctgg tatatgaaga
     6961 gatagcatta ctacaagttc aacacctatt actaaaagta atatttgtgc taaaaaatca
     7021 ttaagctgac tgtattgtac tggattttga aaatcaacta tatatgctcc ccacataagt
     7081 cttagaacat ctatagttcc taaaaatact gctattagaa ctactactgc taaaacacgt
     7141 tcaaatataa aagcaatctt taatataggg ttattctttt tttgtttttg taaactatgc
     7201 atccattacc tccctacttt ataatcatag catctccaaa actgaagaat ctatatcttt
     7261 cttttactgc ctcattatat gcatttaaaa cattatcctt tccacaaata gcacttacta
     7321 acattattag tgtagactct ggtaagtgaa aattagtaat aagtttatct aaaactttaa
     7381 atttataacc tggatatata aatatttctg tccatccact agtttctttc atctttccat
     7441 actcattaca agctgattct atagttctac aactggtagt accaacacat attacatttt
     7501 ttccattttc ctttgctcta tttattttgt cagctgcttc ttggctaacc atataatatt
     7561 ctgaatgcat cttgtgattt aaaacatcct ctacttttac tggtctaaaa gttcctaagc
     7621 caacatgtag agttacaaag gctatatcta cacctttttc ttttatttta tttaacaatt
     7681 cttctgtaaa atgtaatcct gctgtaggtg ctgctgctga accattatgc tttgaataaa
     7741 cagtctggta tctttctttt tcatctagtc tagcagtgat atatggaggt agtggcatat
     7801 ttccaagttc atccaatatt tcttcaaaaa ttccatcata atggaatttt ataattcttg
     7861 aaccttcatc tgataaatct acaacttctc caattagctt tccatttcca aaagaaaatt
     7921 tagtccctac ttttgctctt tttcctggtt ttactaaagc ctgccaagta tcttcctctg
     7981 ttctttttaa taataaaaac tctattttac ctccagtttc aagcttctct cctattaatc
     8041 tagctggtat aactctagta ttatttaata ctaagcaatc tcctggattt aaatattcta
     8101 ttatatctct aaatacttta tgttcaatat tacctgtctc tttatctaat accatcaatc
     8161 ttgagctagc tctatcttct ataggaactt gagctataag ttcctgtggt aagtcaaatt
     8221 taaaatcact tgttttcaat gttaaacttc ctctcttaaa atttctccag ctacaataat
     8281 atcaaaaaaa caacttattt tccacaatat taccataatc tatcaagaaa ttgctatttt
     8341 taacagacac catatccaca atatatagat atttttccac gatatacatg tcttatccac
     8401 aatatataga tacaatacac acaattttta tcgtgtttat ccacagtatc tacagatata
     8461 attaccattt tgtggatatg tttgaatttt tagccatttt agaaatattt taatttaatg
     8521 actcttttat tttataaata ctcgtataat ctatttttat ttttcattag gatatggaat
     8581 ttttaagtgt tcatatgcta atggcatagc tactcttcct ctaggacctc tatttataaa
     8641 tcctaattga agcaaatatg gctcatatac atcttctatt gtatttctat cttctccgat
     8701 agaagctgcc aatgtatcta aacccactgg tcctcctcta aatttttcta ttatagtcat
     8761 aagaagcttc tcatctacaa aatcgagacc caaactatcg actcctaaaa gttccagagc
     8821 atcttttgcc accttatcag ttatttttcc atcagctctg acttgtgcaa aatctctaac
     8881 tctttttagt aatctatttg ctatccttgg agtccctctt gaacgttttg caagttctaa
     8941 tgctccatta gactgtatct ctgcatctaa gatgcttgaa gacctaagta ctatttttga
     9001 aagttcatca actgtataat agtccaattt acaaataaca ccaaatctat ctctaagtgg
     9061 atttgtaagc ataccagctc ttgtagtagc tccaataagt gtaaatttag gaaggtcaag
     9121 tcttatgctt ctagcagatg gccctttccc tattattata tctaggcaaa aatcctccat
     9181 tgctggatat aaaacttctt caacacttct attgattcta tgaatctcat ctataaataa
     9241 aacatcattt tcatttaaat tcgtaagtat ggctgccaaa tctccagctc tttctattgc
     9301 aggtcctgac gtaattctta aatttacccc catctcattt gctattatac ttgctagtgt
     9361 agtttttcca agacctggtg gtccatataa aagcacatgg tctaactgct catttctaga
     9421 tttagctgct tctataaata tactaagttg ttctttggat ttttcttgac ccaaataatc
     9481 ttctaaagtt tttggtctta aactattttc tatatctata tcttccatct tcattgtaga
     9541 agttattatc ctattttcat cttcaaaacc ttgcatttat acccctcctc tcatactttt
     9601 gaaaatctac tttatatatt ttcaaattta cataaactcc ttaaatattg ttttaaaatc
     9661 caaatattat aaaatccaaa tactatttca aggatttact cattatatat gtaagtgctt
     9721 tctttataat agcttctgta ttcataccat cttttttaca tttttgaact gcctcttttg
     9781 attccgacaa agtatagcct aaagctacaa gtgcatctac agactcatct tgtgatatca
     9841 aggtaggttt ttgtgataat aatgttggtt caaactctat gttatttttg tctaccttat
     9901 ccttaagctc taagacgatt ctttctgctg tctttttacc aacaccagga gctttggata
     9961 attttccaat gtcttcactt aaaatatatg ctcctaactg tgctggtgat gcaaatgata
    10021 gtattgaaag cgccacttta ggtcctattt tagatacaga tgttagtaat tcaaacatct
    10081 taagttcttc cctacttaca aaaccacaaa gactcatatc atcttctctt acaattaatt
    10141 tagtatatat ttttgtggct tctcccactt gtaaattcat tattgtattg gatgatacat
    10201 ttattttata tcctatccca ttattttcaa caactatact gtctatataa atttcttcta
    10261 ctgtaccttt tatataacta tacatactta ccacctatat tctttagagt tttttcaagt
    10321 ttgtttgaat gagcatggca aatagccaca gctaaagcat ctgcaacatc atctggtttt
    10381 ggaacttttt ttagacttaa aaatgaagtt accatctgtt gaacttgtgc cttatctgct
    10441 ctcccatatc caactacacc ttgttttact tgaagtggag tgtattcata tacaggcttc
    10501 ccattatgag cacatgcaag catagtaaca cctctagctt gtgcaactgt tatagctgtt
    10561 tttacattct tgttaaagaa taattcttcc attccaactt catctatatt gtaattcttt
    10621 actattgtat caattccttt atacacaagt tccaatcttc tcgatatatt catatgggca
    10681 ggtgttgtaa ctgctccata atcgattact ttaaacttgc tatttttgta ttcaattata
    10741 ccatatccaa ctatggctat acctgggtca atccccaata ttatcatttt ttaaaattcc
    10801 ccctaaaaat aaaagaaata ccaaaatgta tttctcataa aaacttatgt tctaaatcat
    10861 tatatcataa ataatattaa caaatcataa aatgctctat gtaaaatata aaatcaaata
    10921 aaaaagttat atcaaagcaa gattttacct caatataact tttatattta ttacaaataa
    10981 attattgtat ttttatagga attaaatgtg actacaattt taatactaat ctatttaata
    11041 ctaatctatt taatattaaa ttatttatta ttaatttact tagtatttaa cttacttagt
    11101 atttaactta aaataatatt aatttactat taacttgcaa agtcttctaa tcttgaatag
    11161 gcctcttctt cagtatccat aactacacct tttttgattt catcttgaac tgttttagga
    11221 agtgaatcta ctcttatttt tgttttttct actaatgttt tgcttccact atcatcatat
    11281 ttatatagtg ctatatttcc attatcattt tcaatagtat atttaccttt tatagtaaca
    11341 tccattgctt gtacatcttt attatcacta tatttagatt ctaccaatat gatttcattt
    11401 ttatccatac tctttatttt tttagttgga tattcttttt taaaatatga tactatttct
    11461 gacttcgtct tatctaataa tgactctgga actattcctg ccattgtaag tgttctctta
    11521 tagttagttt cattttcgcc ttcactttga agccaaactt cacaattttt atttaatcca
    11581 aaagcttctt ttgacttttc attcatattc gcactagtac ttacaacttc accagtttga
    11641 actccattgt gttttaatct tgtccctata taaactccgc tacttaatac aaatactcca
    11701 accagtatta aggcagatat tttccaagga aataattctt ttttcttctt ttcaaacatt
    11761 gattacgcct cctttttcta ttattttctc caagctgtaa gtttttatac aatttaggta
    11821 aattttagaa gaaaatatat ttatatttca aattactcta agaatagata ttttctaaaa
    11881 ggtagttttc tcttaatggt tatattcttg taattatatg gacataattt ataataaata
    11941 atcgtttagg aggtaataac tttggaaaga ataaactgta atgtaattaa ctgttcacat
    12001 aacaacagcc atatatgtta tgcagataaa atttttataa atggtgaaaa agctcacaat
    12061 gacagacata cttgttgtag ttcattttta gatgaatcta tttatagtaa tttatctagc
    12121 aacgttaata atagaaattg tgaggaatta gtttgtactg taaaaaattg taaatacaac
    12181 aaagatggta gcttatgtac acttgataat atattggttg ctcctgaaac tgatagagta
    12241 aacctttata ttgaaacatg ttgctctagt ttcgaatgta aatagtcata gtataaatat
    12301 aagggtgtca aatgtaatta aactaacata gtgtagagta tctacaacta ttagtttgat
    12361 tatacttgac acccttattt attttaaata aaatttcatt attataacca tgtttataat
    12421 gttctaactg taattaaatt tgataaattt ctataaattg gtataatcaa tatgtaaatt
    12481 gaatttagta aattaatcat aaaaataaaa tatcgagtat atagtgttat actaactaga
    12541 aatgaggtgc ttatgagtac attttatatt caatttcttt attttgtaat ttatagcttt
    12601 ataggatggt gttgtgaaac tacatattgt tcagttttac aaaaacaatt tgtaaataga
    12661 ggttttttaa atggtccatt ttgccctata tatggatttg gagcattagc aatagttgct
    12721 accttgactc cttttgtaca cactattcct ttgttgtttt tgtttagtat aattataact
    12781 agcataatgg agtattgtac gagctttatt ttagaaaaaa tatttaatat gacttggtgg
    12841 gattattcta aacataaatt taatattcac ggaagagttt gtctggaaaa ctcacttatg
    12901 tttggtgtat tatcattgat tgtaatgctt atagtacatc ctattgtggt tgactttatt
    12961 aactctatct ctaaaaatgt gctatttata tttgctatat ctatagaaat ttattttgtt
    13021 ttagatttag ttatcacagt ccatacaatt ttacaattaa atggtaagtt aaaacaaatc
    13081 aatcttataa ttaaggaatt aaaagataag aaagaatact acaaacttat tacacaagaa
    13141 actatagaaa acaaacttga atctttagtt gaaaatagac tggatacttt ggaagatagg
    13201 tttgaaatac ttaaagaaaa agaaaattat cgctatgcta aaaatcgtat tgatgagcta
    13261 aaaaataaac ttaatacttt gctgtctaac cacaaattac ttcatagacg tataatcaaa
    13321 gcattcccca acataagctc taataagcat acagatattt taaataaaat caaagaaaat
    13381 attaaatcaa ttaaaaaaga taaataataa aataaacaca taagttaaat aataaaaata
    13441 ctaatacata agattaaggt ttagaatatt ttttgtaaaa tactctaaac cctaattaat
    13501 atactattat ttttaatctt aatttataat tttagtttat tttaatttta actcagtaaa
    13561 tttaagcgtt taaacttcaa tacactaata tactacccca tcaatatctc aactgattct
    13621 ctaagtgtgc taatctctcc cacatttcca gggaaaatta tatatggcat attaggaaat
    13681 ttgctttctt tgcctgtcat ccatactggt ataccaggtt ttatctgtcc cattactgta
    13741 gcttttttta ctcttagtgc tttagtacca acatcactag aagtaattcc acccttagct
    13801 ataataaaat ttggtcttac attaagcata ccaattatac tggtaactgc atctgatatt
    13861 tctacagaaa tcataagctg tttatcttta tcattcgtat ccaagtcaaa acgctctcgt
    13921 ctagtataaa cagcaacagt tttacccctg cttatttttt cctctacaac ttttataact
    13981 ctttttacct cattttttag accattttct tgaagaacta aatgttgatt aaattctata
    14041 aattctatcg gatatttaca atttttaagt tcttctaact gttgggtagt cttattcaca
    14101 tgtgaaccaa caagtataat tcctccattg gtattacctt ttgatactaa ttcatcctta
    14161 gtaagtagtt ccttgtcact cactccgcca agtatttttg ttatggcagc agcgcttcta
    14221 aacataaatt ccttaccact atttactgct cgtataaacg caattgtaaa tacctttaca
    14281 tctacataat cggttgcatt tacaataatt ttattgaatc cttcagcttt tttaagtttt
    14341 tctgttatag cttcaatatt tagctctcta agttcttttg acgaaatata tatcatatca
    14401 ctagatttgt actttccatt tgtcttttct tcacaccact cacctatatc agatgattta
    14461 tatccaaaag atttatcttt tgcaaattca gtcattcctg ctggtacaag tatttctcct
    14521 tctttaacat aatgtacatt atctatagta aacctaccac cttctttaaa aaatggcatt
    14581 attatttcac cctgaaataa cttctccgat aatctctcta cttcgctttt taataaatta
    14641 gtttcaagag gataatgtcc tctcatagta gaatcactac gacttatgat tataaaatcc
    14701 ttattaactt tctttgatac atccacaata ttctttgaaa tttctttatg tgcttttgta
    14761 gtttgagcaa cagtaaatcc ccttgaattg gtaagtataa aaaacatact atttttctca
    14821 ttaaaacctt gttccatact atccttatcc caatcagtat aaacagaaat atcatgaact
    14881 gtttgtacac ctgttgggtc atcatctaat acaacaattt ttctatctaa atgcttaagc
    14941 tcatctgcaa gaagtttatt taatctgtcc tcatctactt gtttaaaagt attaagtata
    15001 tctatgctaa ttggttttac ttctctcatt taaaacgccc ctttatgtaa tttgtatagt
    15061 ctaaatgtaa tttatatgat ataaacaata gtttgttatt tatagacctg gaagtggtat
    15121 aattccattt ataacactaa gcaataaaac cattataaat gctacaactc cagcaatggt
    15181 tcctccaatt gtccaagagt ttaaagtatc tttaactgat aatccactaa atcttgaaag
    15241 tacccagaat ccagaatcat ttggaaggga gaaaccaact cctccagcac atatagcaag
    15301 ccctactaaa actggtgaca ttccagttgc caaaattgta ggtcctaaaa ttgatgatgt
    15361 tgttactaag gctacagtag cagaaccttg agaaattcta agcaaggcac ttaataagaa
    15421 tccaagcact acaactgata tactaagacc agacatagta tcaactaaga agttacctat
    15481 accacttgca tttataacac tgccaaacgc accacctgac cctgttatca ataatattag
    15541 ccctgctgaa tcagcagctt caataactac atctcccata ggtttattta tgtatttctt
    15601 taaaaatcct attgccacca atattccaag aagtattgct atgtttttat ctcctataaa
    15661 tgcaagtact ccactcattg tagaaccttt ttctaagaac atactaaata catttgatat
    15721 aagtataaga agtattggga acaataaaga aaacatagaa attccaaaac ctggtacttt
    15781 agaagaatca tcatcttcaa tttttatgct atcttttccc tgctctaaat ttttttctct
    15841 atcattctca tcaatagcat aagctggata tttttcaaaa cgttttccat aaatccatcc
    15901 acctataaga gctgctggaa aagcaacaat tatagaataa agtataaaac tacctacatt
    15961 tgcatttaca tttgctgcaa ctgctaaagg tcctggtgta ggtattacaa gtgcatgacc
    16021 tactatagtc ccaactccta aggcacatac aaaaaccatt agagatttct tagttactct
    16081 cgaaacatac ttaattatag gcatcattat aacaaaagct gcatccataa aaactggaat
    16141 tgaaattaaa aaacctgctg tagttactgc taaagcactg tttttttctc cagtcttagc
    16201 taacaacccc tttgcaatag attctgttgc tcttgattct gaaagaatat ttccaaatat
    16261 tattccaagt ccaattacaa tacctatact acctaatgta tttccaaatc cagtcgatat
    16321 tgtggttgat atttcattta taggcattct aaccataaag cctgtaatta tagatgttat
    16381 cagtaatgcc aaaaacggat ttactttaaa tttaataatc gatacaagta aaattcctat
    16441 agatataaca aatgctatca aaagtccata tcctgtaatc ataatcttta cccctttatc
    16501 attctacttt attatttatc aattttattt ttcttttact atgataactt aacctcgctg
    16561 tatttatata atctaatcct aataatatgt atttaattat attgatatat ttaaatatta
    16621 attattgtgt ttttttattt caacacctgc caacttttca aaatactgtg ctattccact
    16681 atggtcatct tccaaatgtc catgtacttt taatgtctgc ataatttcta ataattgact
    16741 tgtcataggt agtggtacat ctaaattgtg tgctgtttgc attacattct taatatcttt
    16801 caaattaata gatattttcc cacctggttt aaaatcacga ttcataatca ttggtatctt
    16861 cgcatccaat attgtactac cagctaggcc tcctcttatt gccttatata ctttttctgg
    16921 gtctgcacct gcttttgctg caagtacaaa tgcctcagaa actgctgcta tcgtcaaatt
    16981 aacaattact tggtttgtaa gttttgtaac acttccacta ccattatctc caattaaaat
    17041 agatgagctt cccattatat taaaaaatgg ttctactttc ttataaattt cttccttacc
    17101 accagccata aaagcaagtg taccatcaat cgcttttggc tcccctccac ttactggtga
    17161 gtctataaat tctaagccca tatcttttaa tttatttgca cacaatatgg attctgtagg
    17221 agtaactgaa ctcatatcga ctactatact tccttctttt aaagtttttg ccaatccatc
    17281 catcccaaac aatatatcct gcacaatagt tccatttgga aggattgtaa atacaatatc
    17341 acattcttga cccatttcta aaacacttgc gtattttgct ccacatgata taagttcatc
    17401 cacagcagat ttattaatgt catacaccaa aagattacat ccatctttta ataaattttt
    17461 tgccattggc tttcccatta ttcctaatcc aataaatccc aatttcattt tataaccctc
    17521 catatcttca catttttgaa aacgtttttt atgttttaga gtatactaca taccagtata
    17581 ccggtcaata taaaatataa aagtatttat tttaacaata aatagtaatt tttaaagtca
    17641 tttattatat ttagtcatcc tatataagtt ttaatacata ctattgtttt caaatataat
    17701 atactacttt tacagttttg tattttaact aatttcaatt agttttaatt aatctgcctt
    17761 taatgttaat catgcttcac acttcaataa ttttttatat tttttaatct tatctgcaat
    17821 ttaaattgca aaagattgac cttattttca ctatgttgta tgcttaaaat tatataagtt
    17881 tttgtatttt tatattgttt aggggggtat tattaagtat ggctacattt aaaagattac
    17941 aagaacaagc atatgactac ttaaaagaat tgatactatc tggagaaatg atagaaaatg
    18001 aaatatattc tgaaacaaaa cttgctagtg aaataggtat ctctagaaca cctataagag
    18061 atgctcttca aagacttagt caagatggat ttatagatat aatcccaagc aaaggattta
    18121 gaatccatca aattacagca aatgaaattg tagagatatt ccaaatacgc tctgcaatag
    18181 aaggattttg tactttttta attacaagtc aatacaaaga agctagagca gtagaaacta
    18241 taagtaaatt aaaacatctt ttagataaac aaaaagatat tttattaggt gataaaaatt
    18301 taaactcatt tgcagaatac gatacattat ttcacacaac tatagtttca tatgctcaaa
    18361 atactgaatt tgacaaaatg tttaacaatt atatgtacag aataaaaaaa ttagctctcg
    18421 attcattgtc acatgaagga aggcttgaaa caacattaaa agagcacaca gatattttta
    18481 ataatattgc aaatggatgt attgaagata tttacagaac tactctcatt catatggaaa
    18541 ctcctaaata cattaattta gaagattttt gtaacaataa gttattttag cattatactt
    18601 tcatttatta aaaatatatc tatctcaaat taatttccta atttataaaa aatattctat
    18661 aattatattg taactaatac ttattctaag gagtgatttt tttgaacgaa gatgttgtaa
    18721 ctaatgaaac tattgacaaa gattatgcta ttgaagaagt tcgtatggca tgcaaacatt
    18781 ttggtgattt atatttttat ttttccaaag tgttatttga agagtttggt gaagataaaa
    18841 ctagcgaaat attaagaaaa gttttatttg aaaggtctga agaaagagct attgctatga
    18901 gagagagagc tcatgaaaat ggagatgaat taattgcaga caatattatt agcacaactg
    18961 atgttccatt tctaggatgg gttcctgaat ttgaagagtt acattgcccc tatggagctt
    19021 cctggctttt aagatttgag gaaaatcctt ggtttaaaaa attcgcttct ctttactgtg
    19081 atgttacaga tactactgta gctgaagtat ttacaggaga tacgtcccat aaaataacaa
    19141 aaaacattct atggggagat aaaagttgtg aaagaatata tttccatgat gacaaggtat
    19201 ctgaaggtaa atatacttat ggaaataaaa actgtaaata aataatttaa attttctata
    19261 ttcatatact atattttgta ctttttcata taaatattat aactagtctt ttattttact
    19321 tttgtttaac ctaaagataa tttactttta atc
//
LOCUS       DBGJFU010000076        19206 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.076, whole genome shotgun sequence.
ACCESSION   DBGJFU010000076 DBGJFU010000000
VERSION     DBGJFU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.076"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            62..562
                     /locus_tag="ACOIAS_002445"
     CDS             62..562
                     /locus_tag="ACOIAS_002445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2702150.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(812..1591)
                     /locus_tag="ACOIAS_002446"
     CDS             complement(812..1591)
                     /locus_tag="ACOIAS_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702151.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1588..2067)
                     /locus_tag="ACOIAS_002447"
     CDS             complement(1588..2067)
                     /locus_tag="ACOIAS_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2702152.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            3053..4060
                     /locus_tag="ACOIAS_002448"
     CDS             3053..4060
                     /locus_tag="ACOIAS_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGZ2702153.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            4098..5525
                     /locus_tag="ACOIAS_002449"
     CDS             4098..5525
                     /locus_tag="ACOIAS_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HGZ2702154.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SAGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            5667..6932
                     /locus_tag="ACOIAS_002450"
     CDS             5667..6932
                     /locus_tag="ACOIAS_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2702155.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTRIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            6974..8572
                     /locus_tag="ACOIAS_002451"
     CDS             6974..8572
                     /locus_tag="ACOIAS_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HGZ2702156.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            8607..9629
                     /locus_tag="ACOIAS_002452"
     CDS             8607..9629
                     /locus_tag="ACOIAS_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2702157.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(10017..11420)
                     /locus_tag="ACOIAS_002453"
     CDS             complement(10017..11420)
                     /locus_tag="ACOIAS_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2702158.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            12305..12796
                     /gene="infC"
                     /locus_tag="ACOIAS_002454"
     CDS             12305..12796
                     /gene="infC"
                     /locus_tag="ACOIAS_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGZ2702159.1"
                     /translation="MNEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLDLVQISP
                     NANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTKANNAIK
                     FLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNNMVVIID
                     PKK"
     gene            12824..13018
                     /gene="rpmI"
                     /locus_tag="ACOIAS_002455"
     CDS             12824..13018
                     /gene="rpmI"
                     /locus_tag="ACOIAS_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGZ2702160.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            13074..13430
                     /gene="rplT"
                     /locus_tag="ACOIAS_002456"
     CDS             13074..13430
                     /gene="rplT"
                     /locus_tag="ACOIAS_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGZ2702161.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            13740..14285
                     /locus_tag="ACOIAS_002457"
     CDS             13740..14285
                     /locus_tag="ACOIAS_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702162.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            14272..14436
                     /locus_tag="ACOIAS_002458"
     CDS             14272..14436
                     /locus_tag="ACOIAS_002458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702163.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLLGLYFIVSFHLS
                     NDLEWYNEVF"
     gene            14725..16650
                     /locus_tag="ACOIAS_002459"
     CDS             14725..16650
                     /locus_tag="ACOIAS_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2702164.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKYTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            17051..18955
                     /locus_tag="ACOIAS_002460"
     CDS             17051..18955
                     /locus_tag="ACOIAS_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2702165.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKI
                     FGKEVNIITTSSLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         7265 a         2165 c         3383 g         6393 t
ORIGIN      
        1 aaaatagaat catataaaat aagttaacta caatcaaaaa ttaaaaaggg gaaatgttaa
       61 gttgatagaa ggcataaaat ttataaaggc agaagaaaag tatatttatt cgtattggca
      121 aacttttgat aaaatagcaa aagagaggaa atatctagct atggatgaag cctttccttt
      181 tgaagaaaca gttgaattta taaaaaatat tataaataaa aacttaccac aactgtttat
      241 aattgattta gaatcagata actgtattgg ttggtgtgac gttttaccta aaactgaaaa
      301 agttggttat ttaggcatgg gtattcttaa agagtatagg gaaaaaggta tcggaagtaa
      361 tcttttaaaa caaattattg atttatcaaa agaatatggg tatgaaaaaa ttgagcttga
      421 tgtatttaaa agtaatagta gagctattca tgtttataaa tctttagggt ttgtagaggt
      481 aaatacaata tctagtggtt ttacatggaa tgatagacct gtaaaagaag aagtaattca
      541 aatggagctt acacttatat aaaataaagt taaagtcttt aaaattccat atacacttgt
      601 ataaaataaa gttaaagccc ttaaaattcc atataaaaga aatattttta agggcttttt
      661 aataatgctt cttttgaagg tagctaaata tctaaaattt tatatattaa atttaaagaa
      721 aatataaaga tggaagatac aaccatgttt actaataaaa aaattaaagt cataaataat
      781 aaactcataa acaacttatt attacttatg tttattttgc atctattact tcatgagcca
      841 ttttaactaa gtcattcata gataaatttg agtcttgaac tgtaaaagaa tagtaaactc
      901 catcttgttt ccatgtcaaa aacttaaatt tattaacttc aactttgtct gattcattat
      961 ttggccctac actaaatgtg tatttgcctg atttttcatc aattttatcc tgttctgtca
     1021 ttttataacc ttctggtaca aacttttgtg cgaatgcttc ataagaaata ttaatatctt
     1081 tgtaagtatc tacaactttt agattgtcag aactcttccc aagtacatta tttgctgtgt
     1141 caagagatat ttcatcattg ccatttttat attcaaaact aagtgctttt gattttccta
     1201 aaacattatt ttctttgtca aatgcttttt tatttacagt atatgctcct ttaaatttat
     1261 atccattact aaattcagaa actatattag gggtaaagtt aaagtctttt tttacttgtt
     1321 ctgtagttgg gatagatgta taggtagcct tagtacttga ttttccaacc aaactatata
     1381 tatgtcctac tgcaagggct gttgtgctca atatcaaagt aatggctaaa gttgcaacta
     1441 agccttttct aaatggtaat ttgtgtgttt tattattaat cattttataa ttttcctctc
     1501 tttctggtaa actatttagt gttttgctta aactattatg aaaactttct ggaacttctg
     1561 gaaacgaatt atttaatata tttttattca tatgattacc tccgtgtttt ctaatttagt
     1621 ttttaataat cttcttcctc ttgataatct actttttaca gttccttgag gtatatctaa
     1681 tatttcctta acttcatcta ctgaataacc ttcaatatag taaaggacta ttggaatttt
     1741 aattttttta ggtagacttt gaatagcagt atacaaatcg ctatagtcat ctttttcatc
     1801 aatttttttg tttttgaaga catcttcaaa tgatagagat tttaatcgtt tgcgtcttaa
     1861 tgaattacat tcatttatga ctattctaat cagccatgtt ttaaagtatt gttcttcctt
     1921 aagagaatcc aacttttcat atgcttttaa aatagcattg ttgacggcat cttcacagtc
     1981 ttggtcgttt cctagaatag acttagatac tcgatacaaa gtctgttctg attcaagtat
     2041 attgtttgta aatgtagttt tatccaacta agttccttcc cttctatgca aatcgtagcg
     2101 ctactttttt gtctttcact tattagataa aattttacta tagatggttc acttttttta
     2161 tgattatttg ataaatttaa gtttgaaagt tttgcctatt ctatacaaat attctataat
     2221 atattttttc tagtattttc ccctcatata taaagccatg tagatatatt aaactttaga
     2281 agccatttat atatttagtt ttatagtaag tctttatatt gagtaattag aaaatagtaa
     2341 gtatttaatg ttgagtagtt agaataacaa tgcttttgga ttatttactt catgtacaga
     2401 tttaaaataa gttctttaaa tagcaacttt ttgaaaaaac gcaaaaatat tttatgttgt
     2461 ctgattatat ttatagatgt gaaacaatat tgtatagagt aaaagtatat agaagatgat
     2521 tacatagagt ttatacttga attttacaaa tataattatc aaaatactgt caaagagaaa
     2581 aatagttttg atgaacgttt ttatcaaatt gctacagaga cagggctaag tgctaatact
     2641 gtatataaag tattcaataa aataataaat agtggaaaaa ctgaatttac atcaaaggaa
     2701 ttagctacta tatgtggtgt aagtattaga actatggaca ggattatact tagattgtgt
     2761 gattctggtt attgtgaggt agtgagtgaa cagcttatga gcaagtatgg tagacccagt
     2821 agaatattac gttttaaacc ttttctgttg ttttaaatag atataagtca aaaattaaat
     2881 ttttatgata acattttctt tacaattttt tagatatatg ttaatattta gaaaaatgca
     2941 tatgtattca taaaataaac ttaaaacagc taaaaaatca ttaaaaagga aattgtatgc
     3001 atgaaatgag aaagttaatt tataacttaa ataaatctgg gaggagataa ttatgggaaa
     3061 atatcaggat tcaaaaaaag ttgtaagaaa ttattttgaa gcattagaaa atgctactgg
     3121 ggatgaggtt gaaaatgtct taaagcaaca tatgaaatca gattataact ggaaaggtgt
     3181 gtatccattt cgtgaacaag aaggcactga aaatgtagca gatgtatttt ggaagccttt
     3241 aaaaaaatca ttgagtaata tgcaaagacg acaagatatt ttcattgcag gaacaaatga
     3301 gattgacaat agtgaatggg taatgagtat ggggcatttt atgggacttt ttgataacga
     3361 ttggcttggc attaaacata ctcgtaaaat gattagttta agatatgcag aatttaactg
     3421 tgttcaagat ggtaaaattg ctaaaactgg attatttgta gatataattg ggtttatgat
     3481 tcaagcagga gttaatccat tgccaccaca aacaggttca tattttgtat atccagggcc
     3541 aattgaccat aatggattac tttttgaaga tgcagatgaa gttgaaggag taaaaacttt
     3601 agcattagta aataaaatgg tagatgattt atctgaatta aatgaaagtg gtgctatggg
     3661 atgcccacca gaaatacttg aaaaatcttg ggctaaagat atgatttggt atggtccagg
     3721 tggaattggt gcttcttata ctatacctag atatcaagag caacaccagc taccatttag
     3781 aaataattta aaaggaaaga ctttcaatgg gcatgtatgt agatttgcag aaggtaattt
     3841 tgcttgtttc tttggatggc ctaatctgac taatgtacca tgtggaggtt ttttaggatt
     3901 accaggtgga gaggttaaag cagatatgca agttgttgat gtttattatc gtaagggcga
     3961 taaacttcaa gaaaactggg tgttaattga tattccttat tggttgaaac aacaagggct
     4021 tgatattctt gagagaacta aaagtatttt taatgcttag ttatgcatac gtattctaaa
     4081 ataaaggggg atataaagtg gcatctacca acaaaaataa tgcaacaagt aaagaagaag
     4141 aaaaagaagt ttctacaagt gggcttgaat catgtacggt taaagaaaac aaagctaaaa
     4201 tgaatcaaac taattttagt aatataaatg aaaatcaaac aaatgagaat aaaacgaaca
     4261 catataaaag aaatatagat aaaggaaata tagataaagg gaatatagat aaaacaaaca
     4321 caaataaatc cgacataaat aaagttaaca tagatacagt ggatacagat aaattaaata
     4381 caaatactct tactaaaaaa aataaaaaag ttatatttaa gggggaagat aatatgtcat
     4441 ctacaaaaat ttactatgga gattcattag aagtaacgcc tgttggagta atggattaca
     4501 atgatttttc aaaacagaca aaaagggaac aggaaatacc tggatttgat tctaagtata
     4561 gagattttgt agattatatt atgaagatta cacataatat ctgggaagaa aaaggtatag
     4621 gtgttattta tgatacatat cacaataatg ttacaatgca ttgtggctct tcaaatttag
     4681 ttggtattaa agatgttatt tccaatactc ttcaaacatt acatgcgttt cctgatagaa
     4741 gattaattgg tcaaaatgta atatggtcta actttggtgc tgatggtttt ttatcttctc
     4801 accgtgtttt gtctacagca actaacttag gtgatagtaa ttttggacca gcaacaggta
     4861 gaaaaataaa ctttagaact gtaattgatt gtgccacaac taacaataga atccacgaag
     4921 aatggttggt tagagataat ttatggattg ttactcaatt aggattaaac cctcaggaag
     4981 tagcaaagag tatggcaaag gcaagtgaat ctaaagtgct tagtttacaa tcaacttatg
     5041 gtatctgtga atctatggat ggtcaattta tgccaactaa gtatcaagca aaagatgatt
     5101 ctgctggcga aatgatgctt gaaatgacaa gccgtattta taattataaa tatataaatg
     5161 aagttaaaaa atactatcat gataatgcag tagtacattt tatatgtgat aaagatttaa
     5221 atggttatga tgagattcaa ggaatgattg taagtttact tgcatcaatt ccaaatggaa
     5281 gttatgaagt ggaaagagta acttgtaatc aaagagaaaa aaatgaaggt tatgatgtat
     5341 ctgtaagatg gagattacgt ggaataaatg aaggtatagg attttttgga cagccttctg
     5401 gaaagcatat agaagtaatg ggaatcaatc attatcatat actacaaggt aaagtaaagg
     5461 aggagtggat aacttttgat ggtatggatg tattaaaaca aatgtatatg ggagtagaag
     5521 aataaagctg tttagagtat aaatagtttt gaaatatata gggaagtatt gctttataaa
     5581 atttaagtgg ggaatgtata tgaaaaatag atgtttaaac ttttagcaaa attatgacta
     5641 aatttaaaag ggggaattat aaaaatatga aaacaacggt tgaaatagaa aatgatatca
     5701 tattaggaaa agactattca aacctaaaaa gaggaattgc atttttttca attgctttaa
     5761 tatacttttt ttattgctac aattttatgg tgggtacttt tattaaacca acaatgattt
     5821 atgctttagc agatggggga tttggttttt cattaaaaca aacagaagaa atatttgcag
     5881 ttatgtcttt tggtacaatt ccaggtacat ttatttttgg ggtgatttct acaaaaattg
     5941 ggaagaagag gactttaatt agcgttgctt tactgattgg attgacaaca tttataccaa
     6001 tgttatctcc tacaaatatt atgttatgga aaatagcacg tttgtgtaca ggagtagtgt
     6061 tgggaggcgt atttggaaca gctatgcctt tagttgcaga tatgtttcca agtaaatatc
     6121 gtggtaagtt agctgctata ttaactgctt tattttcatt agcaatgatt tttggtggga
     6181 aagtatatgg cgttttaggt gatgcaaatt ggcaaatatt gatgtatact gcgattatac
     6241 cacctatagt tggagctatc ttagcaattt tctttgtgcc agatgatttg gagtatacaa
     6301 aggaattagt taaaaaggga aaagaaactg gtgaaaagat tagctatata agtatgtata
     6361 agggaaaata cttatggatt ggattgggaa cgattctttt atcaggtgca aattttacag
     6421 catattcagc attttcaaat aatgcaacta catacctagt gacaagaata ggaatgtcag
     6481 cagcagtggc tgggtctata tatagtttac aaggaattgg tcaattagta ggatatttat
     6541 tctggggttc aattgcagat aaatttggaa gaaaaatacc ttttataggt atggcattag
     6601 cagcagtatt tgtttttata tatacaaaat taggtggaga taatacaaca acattctata
     6661 tgatatcagt attactaggt gttgctgttg gatattctgg agcatgggga gcatactaca
     6721 cagagttgtt cccaagtaaa tatcgttcgt tgtcatctgg tatgtcattt aatggaggaa
     6781 ggattatatc tacgtttgca atacctgcta tagctggtac agcttctggt actttaggta
     6841 tgatgactat cttttacata tcaatcgcag tctttgtagc aggtgctatt gtatggttat
     6901 tcttaccgga gacgttaaat aataaaaaat aaaatactag gaatttattt tgttacttag
     6961 aaaggggtgg cttatgattt catcaattga tgtatgtatt ataataggat atttgctatt
     7021 aatgttagtt attggttatt attcaggaaa agataataaa aatcaagaag attatttctt
     7081 ggcaggtagg tcaatgccat ggattccaat tgctttatca gttgcagcta ctatgattag
     7141 tgcaaatggc tttataggtg gtcctggatg ggcatatgta gatggaatgt atccagtaat
     7201 ggttaatatt acagtacctt tagcaatatt ttttgcactg agcattacca ccccagttat
     7261 ctataaatta aaaataacct ctatatatga atatatggga cttcgtttag gaaattattc
     7321 aagaggatta actattattc aattttttat taattcattg attcaagcta gttctatggt
     7381 atatattcca gtgcttatta ttcaaatgat tactggctgg tcactttatg ctttagtccc
     7441 aatagttgta cttgtatcaa ttatatatac tctattaggt gggattaagg cagttatatg
     7501 gactgatagt attcaaatga tagtagtaat aagtgctgta tttatagtaa ttattgtagc
     7561 cttaaaagga atgaatctaa gtttctttga tacattacaa attgcacaac agagtggaaa
     7621 attaaataca tttgatttca gtagggatat ttcagtgaca aatacttttt gggctacttt
     7681 aattggtgga actgtaatgt ggataagata cttttgtttt gaccaatctc aagtacaaag
     7741 agttttaaca tctaaatctt taaaatgtgc aaaaaattct tttgtagtta gtgcatttgt
     7801 tatgaattta gtttactact ttatgctatt ggtaggagta attttatttg ttttttatgg
     7861 gggaaggaca tttgaaactt caaatgaaat aatgattact tttattttaa atgaacttcc
     7921 agtaggagcc attggaatta ttattgcagg tgtgtttgca gcagcaatgt ctagtatcga
     7981 ctctatatta aacagtatga caactgtttt tacgaaggat atttatgagt tttatttcaa
     8041 aaaagacaac aaagaatcat cattaaaagt tacaatgatt ataactgtag ttataggagt
     8101 tgttatgaca ggttttatta ttatgggatt tggaggaagc atcaaatcaa ttcttgacct
     8161 tgttggtaac tatatctcat actttgctgg accagctaca ggtgcatttg tactagcaat
     8221 gtttacatat aaagctaatg ataaaggagt atctattggt tttattgtag ggttagtttt
     8281 aggatttttt atttctaata aatataatgt gagttggttg tggaatccag caataggtgc
     8341 tacaataaca ttgatatttg gatatatttt tagtgtcatt ttaaagaaaa atcataatgc
     8401 agatgatatt aaaaaataca ctgcatttgg tatgagaaat caccttattt ctcaaggaat
     8461 ggataaagta gatggagtat ctatagtgcc attttcttta ggtaaacaag aactaattgt
     8521 attagctttt tttattatcc aatatattat attatttttg atacaatttt aagaagaaat
     8581 actagatatt taaggagaaa tactaaatga atcaaaagaa agttcattat aataaatcgg
     8641 ctacctcaaa agatgttgca agattggcag gagtatctca atcatctgtt tcaagagcat
     8701 ttggtagtgc aaatggaaag ggcgtaaaac ctgaagtaag agaaagaatt tttcgtgtag
     8761 caaacgaaat aggatatgtt cctaatttgg tagcacgggg aatgattagt ggaaaaacta
     8821 atgttatagg attgatagtt ggtgatagtc taggtccatt ttacaataga attatcaatt
     8881 tatttgtaga aaaaattcaa gagataggta aacagtgtct cgtttttaaa gttcctagac
     8941 aacaaagtat agatagtata atttcaaaag taattcagtt tcaagtagaa gctgtgatta
     9001 ttacagcttc ggccatgaat aaggtgatgg ctgagacttg tgaagaaaac aatattcctg
     9061 tgattctttt taatcgtttt atacctggta taaaaataag tacagtctat gtagacccaa
     9121 ttgaaggtgg gggaatggct gctgaatatc tgcttaagaa aggtcataag aatataggat
     9181 atattcaatt tacgagagaa acaagtgaag aaatggaaaa gaaaattgga ttttattcta
     9241 agttaagaca aagtgggatt cataacctta aagaagaatc tgcaaactat gattatgatt
     9301 ctggatatga agcaggtaag agaatgttag cattatcaaa tcctccaaca gcaatttttt
     9361 gcacaagtga cttgatagct attggtgtaa tggatgctgc aaggtttgag tacaagttga
     9421 gggtgccaga ggatttatca gtgataggat atgatgatat tcaaatggct tcatggaaga
     9481 gttataattt aacgacgatt agacagccac tagatttgtt gattgaaaaa acaattaaca
     9541 tacttaagtc cttattaaat gaagaaaatc cagagtcagt tgttgaaatg ttaaaacctg
     9601 aattgattga aagaggttct acactttaag tggtagatat ttaattaagt ggtagatacc
     9661 taattaagtt gtagatatct gataagaaat ttttaagttt tcaatcgttc taaattgtga
     9721 gtataaaaat ctactaaatt tagttatgta aaatataaat tataagtgta gaaactaggc
     9781 tactagcttt tataccactt caaacattac tggaggtagt taagtttaca tcagtttagt
     9841 tttatgtaga tataatattc tgtaaataga caaaattaaa atatgtaagt gtctcaaaat
     9901 gaaattttta gtttatgagg cacttttttg tataaaatca aatgtatttt tataacttta
     9961 acaataaaaa gaggcttatc tctattttga gacagcctca aatttttaaa actatattag
    10021 tttacagctt cttttcctat caaatctttt aactcttcat gagtcatagt ttctttttca
    10081 aataagaatt tagaaacaga atgtaaatct tcaagattat cctttaacat ctctaaagtc
    10141 tctttataac aaatttcaac tatacgattt gcttcctttt gaaccttatc acataagaaa
    10201 gtcttatcat ttccatcaat agtcataaat cctaagttac tcataccata ttcacaaacc
    10261 atttggttag ctatttcagt gactttcttt aagtcgtcct ttgcaccagt agatttatga
    10321 ttaaatatta attcttctga agcctttcca gcaagtaata tttttatttt tccaatcaac
    10381 tcatccttag tataaatata tctatcttca tcaggtaatt gaagaacata acctaatgct
    10441 tgtcctctag gaactataga tattttttgt ataggacaaa ttcctactat atcacttaca
    10501 agagcatgtc cagcttcatg atatgctact atttttttct ctttttcaac caatgcagag
    10561 tttttagatt ctagccctgc aataactctt tcaagtgcct tgtcaaaatg ttctgatgta
    10621 atacactcac tgtcatctct aactgcaaat atagctgctt catttgctat atttgaaaga
    10681 tgagccccat tgaaaccatg agttttttta gctaaaagtt ctagatttac agatttgtct
    10741 ataggtttgt catctgtatg tacctttaga atttcaaatc tagtgtgata gtttggtgca
    10801 cctatatgaa tgtgtctgtc aaatcttcct ggtctaagta aagcttcatc taataagtca
    10861 agtctattgg tagcacctat tattaataca ttggaatctt tattaaatcc atccatttca
    10921 actaaaagtt ggtttagtgt ttggtctttt tcgttgttac tttcaagatg tctctttgct
    10981 cctacagcat ctatttcgtc tataaatatt atacttggag cttcttttct agctttttca
    11041 aatagagttc taactctttt tgctcccaca cctacatatt tttcaacgaa ctctgaacca
    11101 gtaacattga aaaatgatga gtttgtttca ccagctacag cagatgcaag tagggtttta
    11161 cctgttcctg gaggaccata aaataatact cctttaggta tttttgcacc cattttttga
    11221 tatttttgtg gtgacttcat aaaatcgact atttcaaata gttcttcttt tacttcatct
    11281 aatccagcaa catctctaaa tgtagtcttt ggttttgtag aaagtgaatc atctgtttga
    11341 ttatctcttt ccatttttgt ctgtgcaaag acaggagctg gattgttgat tttcttaaaa
    11401 aagttgttta gcattttcat attcacacct caggtaatta attattgtta atcaatttta
    11461 tattttccat atttactatt ttttattatt tatttttcat ggaaaaaata tacatgttat
    11521 ctaaataaaa ataaatttgt aagatgtatg aaaaaatgaa tttatgtata tagagatttc
    11581 tacttatgta ttgaataaag tattacaaat tataaattat gtattgatta tatattgagt
    11641 aaagtattat aaagcatgta ttgattaagt attgaataaa atattacaaa tttagttgaa
    11701 atgaaaatga taaaaaatct tgtactatat tttacaaatc atataaatag agaaattgtt
    11761 gtagcacaaa gtagtaggta gtatattaga atataatata ggatttgttg tgtagtatag
    11821 tagcatatta gaatataata taggatttgt tatgtagtat agtagtatat tagaatacaa
    11881 tatagaattt gttgggtagt atagtgttat attagaatac aatataggat ttgttatgta
    11941 gaatatatag agtcatgtag aatataaata tataaaagga ttttgggtat aaatttaaaa
    12001 attaatagca aaatgagtag tatagacata taattagtag taggaagtaa ttaaattttt
    12061 cctaaacaaa aaaaatagtt gcaaataatt tgattaagtg atatagtaga taaggtaaga
    12121 ataaaacaaa gaagaagtcc gcttctcacc ttacgccgga ggttagtaag gtaaatataa
    12181 gttaaagctt aattttaatt taagtttgtt taaatttggt aacttatatt gcggatgatt
    12241 acgtcatccg ctttttattt tataaaaatt ttggaggtgt cctacaatta gcaaagaatt
    12301 agcaatcaat gaacaaataa aagataagga aataagagtc ctttcaccaa caggtgaaca
    12361 acttggagta atgcctacta aagaagctca agcaatggct aatagcaaaa acttagattt
    12421 ggttcaaatc tcgccgaatg caaatccacc agtatgtaag ataatggatt atggaaagtt
    12481 tagatatgag caagcgagaa aagagaaaga agctaagaaa aaacaaaaaa ctatagttgt
    12541 aaaagaagta agacttagac caggtattga acaaaatgat ttaaatacga aagctaataa
    12601 tgctataaag ttccttaaaa aaggggataa agtcaaagtt gaacttagat ttagaggaag
    12661 agaattagga cataaagaca taggtaaaga agttatgctt aagtttttag atataataaa
    12721 agagtttgga gaaccaacaa aagcccctgc atttgagggt aataatatgg ttgtaattat
    12781 agatccaaaa aaatagatga ttaactgaga ggaggaattt gtcatgccta aaatgaaaac
    12841 tcatagaggt gcagcaaaga gattaaagaa aacaggaact ggaaagttaa agagagctaa
    12901 agcttttaaa aaacatatat taactaaaaa atctgcaaaa actaaaatga acttaagaaa
    12961 atcaacttta gtaagtgatg gagatgctaa gagaatagca caattattac catactaaga
    13021 tacagattgt aaataataac tatagaagag ggatataaaa ggaggtcata acgatggcaa
    13081 gagttaaaaa agctatgaac gctcgtaaaa agcataagaa aatattaaaa cttgcaaaag
    13141 gattcagagg atctagaagc aaattatata gaccagcaaa tacattcgta atgaaagcat
    13201 taaaaaatgc ttatatagga agaaagttaa agaagagaga cttcagaaaa ctttggatac
    13261 aaagaataaa tgcagctgct agaatgaatg gaatatctta ttcaagatta atgaatggat
    13321 taaaattatc tggtattgaa gttaacagaa aaatgttatc tgaaatggct atacaagacc
    13381 cagaaggatt tgcaaaatta gcagaagttg ctaaagcaaa attagcttag tattgaatca
    13441 tgagatgtaa agtctttgag ttatataaat aattcaagga cttttttatt ttttgaaaaa
    13501 tacttattag tgtcatgtac aaccaatttt ataatgataa ataaaataag caatacaata
    13561 gaattttatg agatttatta taagaattgt tacaattatt tatgatagct ataaaaagtc
    13621 atagtaaaaa tgatattgat gtatgttgat atatgtataa agctttcaat caatcttgaa
    13681 ttaatattat caacatgata atattatctt attaataata taaataaggg ggagataata
    13741 tgaatgaaaa actaatggat tgttttgtaa acccaattaa atgcaagtta ttgattgaaa
    13801 ttttttcaaa taatcgaact acagctaaac aattaggaga aaaatttcaa gatatatcaa
    13861 aggcaacact ttatcgttac cttaataaaa tgttgaaaga tgatattata aaagtagttg
    13921 aagaaaataa aataagggga actgttgaaa aagtatatgc tatgaagtta gatatagctg
    13981 atgatgtgaa caaaattata gatgagaact ctggagaaga atatgctcaa ctgtttactc
    14041 aatatatgat gggattttta agagaatttt atgaatacgc tgaaagaaag gaaaaggata
    14101 tcctaaaaga tggttcagga ttttctgtct gtccagtata tgctagtatt gatgagatag
    14161 ttgaagtgag taaaaaaatt gctagtatac tagaacctct aagaaataac tcgcacgaag
    14221 aaggcagaag aatgcattct ataggagtga taatcactcc acctaaagag gatgaaggga
    14281 aataaatctg atttacatga ggtagagcca atttacacgg aaaatcgttt tatgatttac
    14341 atatgtatcc agagcaaagt atctctgctt ggattgtact tcattgtatc atttcatcta
    14401 tctaatgact tggagtggta caatgaagtt ttttaatttt tcatatcaat gcctaaagaa
    14461 atgaacttta gaccacatat gcaattaggt tacattatac aattagacca gatatacaaa
    14521 ataaaaatca gtatttttat agcagataaa ttgactgtta ttcttcttaa aaatcatcta
    14581 ttagttattg ttagcatatt gaaagaaatt ataggaatct attgaataaa tgaatattgc
    14641 ccataatcat ataaactgct atagtttaaa ataaagcagg acgttaaatt ttatagtaat
    14701 ctaaaaattt aagaggggga ttttatgaaa ataaaatttg ttattatgcc attggtgata
    14761 tttttaatgg tattatttgt ggggtgtcaa agtacaaaag atacagaagt taaagcaaaa
    14821 tatacagaaa ttaaaatatt aggtacaggt gatttgcatt caattttgac agactctatg
    14881 gtttcatatg taaatgagga aagggaaaag aataaaaatc tgcttatggt agatgcggga
    14941 gatttctatg gtacacaaag tagagaaatg tgggaatggt cttctggaaa aaagttaatt
    15001 aatattaaaa gaaatggtag agcagagtat gatgacataa ttaagagtag taaagatgaa
    15061 gtacctatag ttaaagatat ggcaaagttg aaatatgatg ctgtagtttt gggagataat
    15121 gaatttgttt caaatgataa gcaaagtctt gataaactag tttctgattt taaaaataat
    15181 aatatgccat tggtatcagc aaatatatat gaacaatctg gagaaaatta tgtacaacca
    15241 tatgtaatga aaaatattaa gacagatgag ggcaatgtaa aagttggaat actaggtctt
    15301 actataaaag aagtagggga aacccttggt cttgaggatt ttgaaaagga taaaaaagct
    15361 agagagcttg gagaacaagc tgattacaaa ggaaaattat atgcaaatga tttagtagaa
    15421 gatgcaaaga aatggataaa agttatggaa aaagaaagtc cagacataat agttgcagtt
    15481 gtacactcag gggaagaacc taaaacagat agaaatccag gaaatagaat aaaagagtta
    15541 gcaactacag tggatggaat agatgcaatt gtagctggtc atactcatga aaaaatagat
    15601 gaacacaaat ataaaaataa atctggagat gaagtaattg ttacacaacc aggttggcat
    15661 ggagatagag tatcagagat taatttcaaa ttaaataaag agaatggaaa gtgggacata
    15721 aaggaaaagt ctagtaaatt aaaaataatt gataaagaag taagtatagt tgctactgca
    15781 gatttacatt cacatttctc tgataaatta acagttgacc ttgttaatga acgtagtaac
    15841 ccaattgaac ctgttgtagt agatgcaggc gacttcttag accctcaaac agatgaaatg
    15901 actcaatggt ataaagagtg gaaaagtata agagataata atagtgataa aacgattagt
    15961 agatgcccaa tggtcatgga tatgaatcag ggaatgtatg atgcagtagt acttggaaat
    16021 catgaatttg tatccgaaaa agatgaattt tggtcagatg aacgcctttt agatgctgtt
    16081 atagaggatt ttgaacaggt ggcaatttct gtgttatcgg caaatatata taggcaatct
    16141 ggtaaaaatt atgttaaacc atatattata aaagatgtgg agacgagtga aggtaatgta
    16201 aaagtaggaa tattgggact tactataaaa gaagtaggta aaggtcttaa aaatgtaaac
    16261 ttacaagagc aatttcaata taaggataaa ttgtatgcaa atgatttagt ggaagatgca
    16321 aagaaatggg taaaagttat gaaaaagaaa aaaccagata taatagttgc agttgtacat
    16381 tcaggagaag aaccaaaaaa tccaaaacat ccaggaaata gaataaaaga actagcaact
    16441 acagtagatg gaatagatgc aattgttgct agtcatactc atgaaaaaat agatgagcat
    16501 gaatataaaa ataagtctgg agaaaaagtt atagtgacac aaccaggtga gcatggtaaa
    16561 tattattcta aaattacctt taaagtaaat aaagaaaagg gttcgtggat tattaatgat
    16621 aaattcagta aaacaattga ggttgaataa ctttataaaa tttatagata aagtaaatta
    16681 tctgatattg acatgatata taagataaag tttctaatat tagtataatg cataatgtga
    16741 aatattttaa taataagaga ataaacaaga caaataaaaa tattttaaca tgtaagatat
    16801 agtatgaact attatactaa tgcttacttt ataatttgaa catgttagaa taagtcttat
    16861 ttgaaaattt tagtgtatta gagtaagtat tattttacca gtaaaaaata ttgagaacta
    16921 tatgtagatt tgcttaaaaa ggaatttaca tatagttctt ttcgtataat aaatagtaca
    16981 atcttatatg gtaaagataa agtgtgatat taaattttat tatcatccaa aatctaagga
    17041 ggagtctctt atgaaaataa aatttattat agtagcatca ataatatttt taatagtatt
    17101 acttgtgggg tgtcaaagta ctaaagacac agaaatcaat gtactcgcca caagtgattt
    17161 acattcaatt attccagata atttaatttc ctatgtagaa gaggagagaa aatatgatga
    17221 gaatctactt atggtagatg caggagattt ttttgacatg caaagtggtg agatgaataa
    17281 gtggtttact ggtcaaaaat tagtaagatt taaagatggg ataccagagt acaaaaaaat
    17341 agctgaacca cgtgaaggag aagttccatt ggctaaaaaa atggcaaagc tgaagtatga
    17401 tgcagttaca tttgggaatc atgaatttgt atctaatgac aagcagagcc ttgataaatt
    17461 ggtttctgat tttaaaaata acaatatacc attagtatca gcaaacgtat atgaacaatc
    17521 tggagaaaac tacgtaaagc catatgtaat gaaaaatata aatactgaca agggaactgt
    17581 aaaggttggt atacttggtc tgactataaa agaagtaggg gaacgttcaa ggtttaaaag
    17641 ttttgaacaa gataaaaagg ctagagaact tggggaacaa cctcaataca aagggaaatt
    17701 atatgcaaat gatttagtag aagatgcaaa taagtgggta aaggttatgg aaaaaaaaga
    17761 aaagccagat ataatagttg ccattgtaca ctcaggtgaa gagccaaaaa atcctaaaaa
    17821 tccaggaaat agaataaaag aactagcaac aactgtagag ggaatagatg ctattgtagc
    17881 aggtcataca catgaaaaaa tagaacagca tacatataaa aataattctg gtgaagaagt
    17941 aatagtaaca cagcctggag ctcatggtaa tagtatatca aagattaatt ttaagttgga
    18001 taagaagaat gataagtggt ttataaaaga caaatatagt gaattaaaga tttttggaaa
    18061 ggaagtaaat ataattacaa cttctagtct gaatccaaaa ttttcagatg aaatggcagt
    18121 taatttgttt aatgaaagag atagagatgt tcaacctata tttatagata ctggagattt
    18181 tttaaatact aatacaaaag aaatgactaa gtgggctaaa gagtggcaat acaatgaaaa
    18241 taaaggcatc tataaaaaaa taaatgaatg gccaattgta tatcttggtt atgatgctgt
    18301 agtacttggt agccatgaat ttacagagga taaagagact tttgaggaaa aaaaatctac
    18361 attggattat attatagaaa actttgaaga aatgaagata ccagtattat cagctaatat
    18421 atatgatgaa tctggagaaa attatgtaaa accttatata attcataatg tagaaactag
    18481 ggaaggaaat ataaaggttg gagtattagg tcttactact actgataagg atgaagaaga
    18541 cttaagtagc tcaagcttaa gcaaagactc aaagacttcg aaaattcctg aagaaacagg
    18601 aaagttatat actaataatc tagtaaaaga tgcaagcgaa tgggtaaaag ttatgcaaaa
    18661 ggaaagtcct gatgtgattg ttgcagttgt acattcagat aataatagta ctctaaaaaa
    18721 tccaagtagt gaagtcaaaa aacttgcaat gtcagtagat ggaatagatg ctattgttgt
    18781 aggtcatacg aaaaataagg tagaagaaca tatttacaaa aataagtctg gggatgaggt
    18841 tatagtaaca caatctggag aagattgtta ttcaaaaata agtcttgaac taagaaaaga
    18901 aaatggcaag tggaatatca taaacaaata tagtaaagca ataaaaatga agtgaattta
    18961 ttgatatata aaccattatt aacactgatt tttataatat aataaatact gattctataa
    19021 tataattaac actacttttt ataatgtcat caacactgat tctataatat aattaatact
    19081 acttttttaa atataatcaa tactgattcc ataatataat taacactact tttttaaata
    19141 taatcaatac tgattctaca atattattaa tactactttt tataatataa tcaacactac
    19201 ttttta
//
LOCUS       DBGJFU010000077        18748 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.077, whole genome shotgun sequence.
ACCESSION   DBGJFU010000077 DBGJFU010000000
VERSION     DBGJFU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.077"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            139..693
                     /gene="tcdR"
                     /locus_tag="ACOIAS_002461"
     CDS             139..693
                     /gene="tcdR"
                     /locus_tag="ACOIAS_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HGZ2702166.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1011..8111
                     /gene="tcdB"
                     /locus_tag="ACOIAS_002462"
                     /gene_synonym="toxB"
     CDS             1011..8111
                     /gene="tcdB"
                     /locus_tag="ACOIAS_002462"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Toxigenic strains produce this toxin (TcdB) or
                     TcdA. TcdB-producing strains are tumorigenic in mice;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HGZ2702167.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8233..8733
                     /gene="tcdE"
                     /locus_tag="ACOIAS_002463"
     CDS             8233..8733
                     /gene="tcdE"
                     /locus_tag="ACOIAS_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HGZ2702168.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8842..8973
                     /locus_tag="ACOIAS_002464"
     CDS             8842..8973
                     /locus_tag="ACOIAS_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702169.1"
                     /translation="MPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKDGAWSVGKIY"
     gene            9059..9244
                     /locus_tag="ACOIAS_002465"
                     /pseudo
     CDS             9059..9244
                     /locus_tag="ACOIAS_002465"
                     /inference="COORDINATES: protein motif:HMM:NF022413.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9461..17593
                     /gene="tcdA"
                     /locus_tag="ACOIAS_002466"
                     /gene_synonym="toxA"
     CDS             9461..17593
                     /gene="tcdA"
                     /locus_tag="ACOIAS_002466"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HGZ2702170.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17928..18626)
                     /gene="tcdC"
                     /locus_tag="ACOIAS_002467"
     CDS             complement(17928..18626)
                     /gene="tcdC"
                     /locus_tag="ACOIAS_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HGZ2702171.1"
                     /translation="MFSKKNDGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         7404 a         2072 c         2902 g         6370 t
ORIGIN      
        1 ttaatgtatt aataatgcat attttcatat aaaatttaat ttatttgccg attatataat
       61 tataatgact gatttaattc cgatgttgtc aaaattttca aataaatcat cattataaat
      121 ataagagagg atgattttat gcaaaagtct ttttatgaat taattgtttt agcaagaaat
      181 aactcagtag atgatttgca agaaatttta tttatgttta agccattagt aaaaaagctt
      241 agtagagttt tacattatga agagggagaa acagatttaa taatattttt tattgaatta
      301 ataaaaaata ttaaattaag tagcttttca gaaaaaagcg atgctattat agtcaaatat
      361 attcataaat cattactgaa taagactttt gagttgtcta gaagatattc taaaatgaag
      421 tttaattttg tagaatttga tgaaaatatc ttaaatatga aaaataatta tcaaagtaag
      481 tctatttttg aggaagatat ttgttttttc ggatatattt tgaaagaatt atctggtatt
      541 caaagaaaag ttatttttta taaatattta aaaggatatt ctgatagaga aatatcagtg
      601 aaattaaaaa tatctagaca agctgttaat aaggctaaaa atagagcatt taaaaaaata
      661 aaaaaagact atgaaaatta ttttaacttg taattaatga gcttaaagaa atatttacaa
      721 tagaaatcaa attttagaat taactttatt gtaaaatcaa taacttaatc taagaatatc
      781 ttaattttta tattttatat agaacaaagt ttacatattt atttcagaca acgtctttat
      841 tcaatcgaag agcaaattaa tcaactgagt gtcttcaatt taaaatgtta ggaagtgaat
      901 gtatatgaaa acctaagtag atattagtat attttataaa tagaaaggag gatatataaa
      961 agagttttat catttagatg taaaaatatt atagtaaagg agaaaatttt atgagtttag
     1021 ttaatagaaa acagttagaa aaaatggcaa atgtaagatt tcgtactcaa gaagatgaat
     1081 atgttgcaat attggatgct ttagaagaat atcataatat gtcagagaat actgtagtcg
     1141 aaaaatattt aaaattaaaa gatataaata gtttaacaga tatttatata gatacatata
     1201 aaaaatctgg tagaaataaa gccttaaaaa aatttaagga atatctagtt acagaagtat
     1261 tagagctaaa gaataataat ttaactccag ttgagaaaaa tttacatttt gtttggattg
     1321 gaggtcaaat aaatgacact gctattaatt atataaatca atggaaagat gtaaatagtg
     1381 attataatgt taatgttttt tatgatagta atgcattttt gataaacaca ttgaaaaaaa
     1441 ctgtagtaga atcagcaata aatgatacac ttgaatcatt tagagaaaac ttaaatgacc
     1501 ctagatttga ctataataaa ttcttcagaa aacgtatgga aataatttat gataaacaga
     1561 aaaatttcat aaactactat aaagctcaaa gagaagaaaa tcctgaactt ataattgatg
     1621 atattgtaaa gacatatctt tcaaatgagt attcaaagga gatagatgaa cttaatacct
     1681 atattgaaga atccttaaat aaaattacac agaatagtgg aaatgatgtt agaaactttg
     1741 aagaatttaa aaatggagag tcattcaact tatatgaaca agagttggta gaaaggtgga
     1801 atttagctgc tgcttctgac atattaagaa tatctgcatt aaaagaaatt ggtggtatgt
     1861 atttagatgt tgatatgtta ccaggaatac aaccagactt atttgagtct atagagaaac
     1921 ctagttcagt aacagtggat ttttgggaaa tgacaaagtt agaagctata atgaaataca
     1981 aagaatatat accagaatat acctcagaac attttgacat gttagacgaa gaagttcaaa
     2041 gtagttttga atctgttcta gcttctaagt cagataaatc agaaatattc tcatcacttg
     2101 gtgatatgga ggcatcacca ctagaagtta aaattgcatt taatagtaag ggtattataa
     2161 atcaagggct aatttctgtg aaagactcat attgtagcaa tttaatagta aaacaaatcg
     2221 agaatagata taaaatattg aataatagtt taaatccagc tattagcgag gataatgatt
     2281 ttaatactac aacgaatacc tttattgata gtataatggc tgaagctaat gcagataatg
     2341 gtagatttat gatggaacta ggaaagtatt taagagttgg tttcttccca gatgttaaaa
     2401 ctactattaa cttaagtggc cctgaagcat atgcggcagc ttatcaagat ttattaatgt
     2461 ttaaagaagg cagtatgaat atccatttga tagaagctga tttaagaaac tttgaaatct
     2521 ctaaaactaa tatttctcaa tcaactgaac aagaaatggc tagcttatgg tcatttgacg
     2581 atgcaagagc taaagctcaa tttgaagaat ataaaaggaa ttattttgaa ggttctcttg
     2641 gtgaagatga taatcttgat ttttctcaaa atatagtagt tgacaaggag tatcttttag
     2701 aaaaaatatc ttcattagca agaagttcag agagaggata tatacactat attgttcagt
     2761 tacaaggaga taaaattagt tatgaagcag catgtaactt atttgcaaag actccttatg
     2821 atagtgtact atttcagaaa aatatagaag attcagaaat tgcatattat tataatcctg
     2881 gagatggtga aatacaagaa atagacaagt ataaaattcc aagtataatt tctgatagac
     2941 ctaagattaa attaacattt attggtcatg gtaaagatga atttaatact gatatatttg
     3001 caggttttga tgtagattca ttatccacag aaatagaagc agcaatagat ttagctaaag
     3061 aggatatttc tcctaagtca atagaaataa atttattagg atgtaatatg tttagctact
     3121 ctatcaacgt agaggagact tatcctggaa aattattact taaagttaaa gataaaatat
     3181 cagaattaat gccatctata agtcaagact ctattatagt aagtgcaaat caatatgaag
     3241 ttagaataaa tagtgaagga agaagagaat tattggatca ttctggtgaa tggataaata
     3301 aagaagaaag tattataaag gatatttcat caaaagaata tatatcattt aatcctaaag
     3361 aaaataaaat tacagtaaaa tctaaaaatt tacctgagct atctacatta ttacaagaaa
     3421 ttagaaataa ttctaattca agtgatattg aactagaaga aaaagtaatg ttaacagaat
     3481 gtgagataaa tgttatttca aatatagata cgcaaattgt tgaggaaagg attgaagaag
     3541 ctaagaattt aacttctgac tctattaatt atataaaaga tgaatttaaa ctaatagaat
     3601 ctatttctga tgcactatgt gacttaaaac aacagaatga attagaagat tctcatttta
     3661 tatcttttga ggacatatca gagactgatg agggatttag tataagattt attaataaag
     3721 aaactggaga atctatattt gtagaaactg aaaaaacaat attctctgaa tatgctaatc
     3781 atataactga agagatttct aagataaaag gtactatatt tgatactgta aatggtaagt
     3841 tagtaaaaaa agtaaattta gatactacac acgaagtaaa tactttaaat gctgcatttt
     3901 ttatacaatc attaatagaa tataatagtt ctaaagaatc tcttagtaat ttaagtgtag
     3961 caatgaaagt ccaagtttac gctcaattat ttagtactgg tttaaatact attacagatg
     4021 cagccaaagt tgttgaatta gtatcaactg cattagatga aactatagac ttacttccta
     4081 cattatctga aggattacct ataattgcaa ctattataga tggtgtaagt ttaggtgcag
     4141 caatcaaaga gctaagtgaa acgagtgacc cattattaag acaagaaata gaagctaaga
     4201 taggtataat ggcagtaaat ttaacaacag ctacaactgc aatcattact tcatctttgg
     4261 gggtagctag tggatttagt atacttttag ttcctttagc aggaatttca gcaggtatac
     4321 caagcttagt aaacaatgaa cttgtacttc gagataaggc aacaaaggtt gtagattatt
     4381 ttaaacatgt ttcattagtt gaaactgaag gagtatttac tttattagat gataaaataa
     4441 tgatgccaca agatgattta gtgatatcag aaatagattt taataataat tcaatagttt
     4501 taggtaaatg tgaaatctgg agaatggaag gtggttcagg tcatactgta actgatgata
     4561 tagatcactt cttttcagca ccatcaataa catatagaga gccacactta tctatatatg
     4621 acgtattgga agtacaaaaa gaagaacttg atttgtcaaa agatttaatg gtattaccta
     4681 atgctccaaa tagagtattt gcttgggaaa caggatggac accaggttta agaagcttag
     4741 aaaatgatgg cacaaaactg ttagaccgta taagagataa ctatgaaggt gagttttatt
     4801 ggagatattt tgcttttata gctgatgctt taataacaac attaaaacca agatatgaag
     4861 atactaatat aagaataaat ttagatagta atactagaag ttttatagtt ccaataataa
     4921 ctacagaata tataagagaa aaattatcat attctttcta tggttcagga ggaacttatg
     4981 cattgtctct ttctcaatat aatatgggta taaatataga attaagtgaa agtgatgttt
     5041 ggattataga tgttgataat gttgtgagag atgtaactat agaatctgat aaaattaaaa
     5101 aaggtgattt aatagaaggt attttatcta cactaagtat tgaagagaat aaaattatct
     5161 taaatagcca tgagattaat ttttctggtg aggtaaatgg aagtaatgga tttgtttctt
     5221 taacattttc aattttagaa ggaataaatg caattataga agttgattta ttatctaaat
     5281 catataaatt acttatttct ggcgaattaa aaatattgat gttaaattca aatcatattc
     5341 aacagaaaat agattatata ggattcaata gcgaattaca gaaaaatata ccatatagct
     5401 ttgtagatag tgaaggaaaa gagaatggtt ttattaatgg ttcaacaaaa gaaggtttat
     5461 ttgtatctga attacctgat gtagttctta taagtaaggt ttatatggat gatagtaagc
     5521 cttcatttgg atattatagt aataatttga aagatgtcaa agttataact aaagataatg
     5581 ttaatatatt aacaggttat tatcttaagg atgatataaa aatctctctt tctttgactc
     5641 tacaagatga aaaaactata aagttaaata gtgtgcattt agatgaaagt ggagtagctg
     5701 agattttgaa gttcatgaat agaaaaggta atacaaatac ttcagattct ttaatgagct
     5761 ttttagaaag tatgaatata aaaagtattt tcgttaattt cttacaatct aatattaagt
     5821 ttatattaga tgctaatttt ataataagtg gtactacttc tattggccaa tttgagttta
     5881 tttgtgatga aaatgataat atacaaccat atttcattaa gtttaataca ctagaaacta
     5941 attatacttt atatgtagga aatagacaaa atatgatagt ggaaccaaat tatgatttag
     6001 atgattctgg agatatatct tcaactgtta tcaatttctc tcaaaagtat ctttatggaa
     6061 tagacagttg tgttaataaa gttgtaattt caccaaatat ttatacagat gaaataaata
     6121 taacgcctgt atatgaaaca aataatactt atccagaagt tattgtatta gatgcaaatt
     6181 atataaatga aaaaataaat gttaatatca atgatctatc tatacgatat gtatggagta
     6241 atgatggtaa tgattttatt cttatgtcaa ctagtgaaga aaataaggtg tcacaagtta
     6301 aaataagatt cgttaatgtt tttaaagata agactttggc aaataagcta tcttttaact
     6361 ttagtgataa acaagatgta cctgtaagtg aaataatctt atcatttaca ccttcatatt
     6421 atgaggatgg attgattggc tatgatttgg gtctagtttc tttatataat gagaaatttt
     6481 atattaataa ctttggaatg atggtatctg gattaatata tattaatgat tcattatatt
     6541 attttaaacc accagtaaat aatttgataa ctggatttgt gactgtaggc gatgataaat
     6601 actactttaa tccaattaat ggtggagctg cttcaattgg agagacaata attgatgaca
     6661 aaaattatta tttcaaccaa agtggagtgt tacaaacagg tgtatttagt acagaagatg
     6721 gatttaaata ttttgcccca gctaatacac ttgatgaaaa cctagaagga gaagcaattg
     6781 attttactgg aaaattaatt attgacgaaa atatttatta ttttgatgat aattatagag
     6841 gagctgtaga atggaaagaa ttagatggtg aaatgcacta ttttagccca gaaacaggta
     6901 aagcttttaa aggtctaaat caaataggtg attataaata ctatttcaat tctgatggag
     6961 ttatgcaaaa aggatttgtt agtataaatg ataataaaca ctattttgat gattctggtg
     7021 ttatgaaagt aggttacact gaaatagatg gcaagcattt ctactttgct gaaaacggag
     7081 aaatgcaaat aggagtattt aatacagaag atggatttaa atattttgct catcataatg
     7141 aagatttagg aaatgaagaa ggtgaagaaa tctcatattc tggtatatta aatttcaata
     7201 ataaaattta ctattttgat gattcattta cagctgtagt tggatggaaa gatttagagg
     7261 atggttcaaa gtattatttt gatgaagata cagcagaagc atatataggt ttgtcattaa
     7321 taaatgatgg tcaatattat tttaatgatg atggaattat gcaagttgga tttgtcacta
     7381 taaatgataa agtcttctac ttctctgact ctggaattat agaatctgga gtacaaaaca
     7441 tagatgacaa ttatttctat atagatgata atggtatagt tcaaattggt gtatttgata
     7501 cttcagatgg atataaatat tttgcacctg ctaatactgt aaatgataat atttacggac
     7561 aagcagttga atatagtggt ttagttagag ttggggaaga tgtatattat tttggagaaa
     7621 catatacaat tgagactgga tggatatatg atatggaaaa tgaaagtgat aaatattatt
     7681 tcaatccaga aactaaaaaa gcatgcaaag gtattaattt aattgatgat ataaaatatt
     7741 attttgatga gaagggcata atgagaacgg gtcttatatc atttgaaaat aataattatt
     7801 actttaatga gaatggtgaa atgcaatttg gttatataaa tatagaagat aagatgttct
     7861 attttggtga agatggtgtc atgcagattg gagtatttaa tacaccagat ggatttaaat
     7921 actttgcaca tcaaaatact ttggatgaga attttgaggg agaatcaata aactatactg
     7981 gttggttaga tttagatgaa aagagatatt attttacaga tgaatatatt gcagcaactg
     8041 gttcagttat tattgatggt gaggagtatt attttgatcc tgatacagct caattagtga
     8101 ttagtgaata gataaaaata tgttaaatat atcctcttat acttaaatat ataaaaataa
     8161 acaaaatgat acactacata aagtgttcta tctaatatga agatttacca ataaaaaggt
     8221 ggactatgat gaatgcacag tagttcacct ttttatattt ctaatggtaa caaaatattt
     8281 ttttatataa acctaggagg cgttatgaat atgacaatat cttttttatc agagcatata
     8341 tttataaagt tagtaatttt aactatatca tttgatacat tattaggatg tttaagtgca
     8401 ataaaaagtc gtaaatttaa ttctagtttt ggaatagatg gaggaatcag aaaagtagca
     8461 atgatagcat gtatattttt tttatcagta gttgacattc ttacaaagtt taacttttta
     8521 tttatgttac cacaagattg tatcaatttt ttaagactaa aacatcttgg aatatctgaa
     8581 tttttctcta ttttatttat tttatatgaa agtgtaagta tattaaaaaa tatgtgctta
     8641 tgtggattac cagtacctaa gagattaaag gaaaaaatag caattttact agatgcaatg
     8701 acagatgaaa tgaatgctaa ggatgaaaag taagtaatgg tagatataat aaagatatta
     8761 acaaataaaa agtgttatcc aaataagaat agctgaaagt tatcataatt catgaaacta
     8821 ataatgaaaa cgagggagca gatgccaaga gacacacaag tattaaatac atataatttc
     8881 gaagcaagtg ttcattacta tatagatgac aaggtagtat atcaaacatt ggttcacaaa
     8941 gatggtgcat ggtcagttgg taaaatctat taagctacat tagttacaga tatcacaaac
     9001 tataatagtt aaacatagaa atatgtgtaa attgtgatgg aaattattca aaaacacaaa
     9061 aatacgtgat gaaggacaaa atgatataga aaataagtat caaaccttaa taaatgattt
     9121 aattgatagt ttaaaagtta taggaaaaat atataaagaa ataaaaacat taaaaaaata
     9181 taagatatgt ttacaaatta ctatcagaca atctccttat ctaatagaag agtcaattaa
     9241 ctaattgagt atctttaaat tgaaatgtta ggaagtgatt taaatatgaa aacttaaatt
     9301 ataaaaaatc aatattaatt tatttttaaa aaatagaaag gagtgtataa gatttatttt
     9361 caaagtttaa aaacaagaaa atcaatttaa atttcagaag gaataaatgt ggttatagaa
     9421 gtggatttat tatcaaaaat aataatacta ggaggttttt atgtctttaa tatctaaaga
     9481 agagttaata aaactcgcat atagcattag accaagagaa aatgagtata aaactatact
     9541 aactaattta gacgaatata ataagttaac tacaaacaat aatgaaaata aatatttaca
     9601 attaaaaaaa ctaaatgaat caattgatgt ttttatgaat aaatataaaa cttcaagcag
     9661 aaatagagca ctctctaatc taaaaaaaga tatattaaaa gaagtaattc ttattaaaaa
     9721 ttccaataca agccctgtag aaaaaaattt acattttgta tggataggtg gagaagtcag
     9781 tgatattgct cttgaataca taaaacaatg ggctgatatt aatgcagaat ataatattaa
     9841 actgtggtat gatagtgaag cattcttagt aaatacacta aaaaaggcta tagttgaatc
     9901 ttctaccact gaagcattac agctactaga ggaagagatt caaaatcctc aatttgataa
     9961 tatgaaattt tacaaaaaaa ggatggaatt tatatatgat agacaaaaaa ggtttataaa
    10021 ttattataaa tctcaaatca ataaacctac agtacctaca atagatgata ttataaagtc
    10081 tcatctagta tctgaatata atagagatga aactgtatta gaatcatata gaacaaattc
    10141 tttgagaaaa ataaatagta atcatggtat agatatcagg gctaatagtt tgtttacaga
    10201 acaagagtta ttaaatattt atagtcagga gttgttaaat cgtggaaatt tagctgcagc
    10261 atctgacata gtaagattat tagccctaaa aaattttggc ggagtatatt tagatgttga
    10321 tatgcttcca ggtattcact ctgatttatt taaaacaata tctagaccta gctctattgg
    10381 actagaccgt tgggaaatga taaaattaga ggctattatg aagtataaaa aatatataaa
    10441 taattataca tcagaaaact ttgataaact tgatcaacaa ttaaaagata attttaaact
    10501 cattatagaa agtaaaagtg aaaaatctga gatattttct aaattagaaa atttaaatgt
    10561 atctgatctt gaaattaaaa tagctttcgc tttaggcagt gttataaatc aagccttgat
    10621 atcaaaacaa ggttcatatc ttactaacct agtaatagaa caagtaaaaa atagatatca
    10681 atttttaaac caacacctta acccagccat agagtctgat aataacttca cagatactac
    10741 taaaattttt catgattcat tatttaattc agctaccgca gaaaactcta tgtttttaac
    10801 aaaaatagca ccatacttac aagtaggttt tatgccagaa gctcgctcca caataagttt
    10861 aagtggtcca ggagcttatg cgtcagctta ctatgatttc ataaatttac aagaaaatac
    10921 tatagaaaaa actttaaaag catcagattt aatagaattt aaattcccag aaaataatct
    10981 atctcaattg acagaacaag aaataaatag tctatggagc tttgatcaag caagtgcaaa
    11041 atatcaattt gagaaatatg taagagatta tactggtgga tctctttctg aagacaatgg
    11101 ggtagacttt aataaaaata ctgccctcga caaaaactat ttattaaata ataaaattcc
    11161 atcaaacaat gtagaagaag ctggaagtaa aaattatgtt cattatatca tacagttaca
    11221 aggagatgat ataagttatg aagcaacatg caatttattt tctaaaaatc ctaaaaatag
    11281 tattattata caacgaaata tgaatgaaag tgcaaaaagc tactttttaa gtgatgatgg
    11341 agaatctatt ttagaattaa ataaatatag gatacctgaa agattaaaaa ataaggaaaa
    11401 agtaaaagta acctttattg gacatggtaa agatgaattc aacacaagcg aatttgctag
    11461 attaagtgta gattcacttt ccaatgagat aagttcattt ttagatacca taaaattaga
    11521 tatatcacct aaaaatgtag aagtaaactt acttggatgt aatatgttta gttatgattt
    11581 taatgttgaa gaaacttatc ctgggaagtt gctattaagt attatggaca aaattacttc
    11641 cactttacct gatgtaaata aaaattctat tactatagga gcaaatcaat atgaagtaag
    11701 aattaatagt gagggaagaa aagaacttct ggctcactca ggtaaatgga taaataaaga
    11761 agaagctatt atgagcgatt tatctagtaa agaatacatt ttttttgatt ctatagataa
    11821 taagctaaaa gcaaagtcca agaatattcc aggattagca tcaatatcag aagatataaa
    11881 aacattatta cttgatgcaa gtgttagtcc tgatacaaaa tttattttaa ataatcttaa
    11941 gcttaatatt gaatcttcta ttggtgatta catttattat gaaaaattag agcctgttaa
    12001 aaatataatt cacaattcta tagatgattt aatagatgag ttcaatctac ttgaaaatgt
    12061 atctgatgaa ttatatgaat taaaaaaatt aaataatcta gatgagaagt atttaatatc
    12121 ttttgaagat atctcaaaaa ataattcaac ttactctgta agatttatta acaaaagtaa
    12181 tggtgagtca gtttatgtag aaacagaaaa agaaattttt tcaaaatata gcgaacatat
    12241 tacaaaagaa ataagtacta taaagaatag tataattaca gatgttaatg gtaatttatt
    12301 ggataatata cagttagatc atacttctca agttaataca ttaaacgcag cattctttat
    12361 tcaatcatta atagattata gtagcaataa agatgtactg aatgatttaa gtacctcagt
    12421 taaggttcaa ctttatgctc aactatttag tacaggttta aatactatat atgactctat
    12481 ccaattagta aatttaatat caaatgcagt aaatgatact ataaatgtac tacctacaat
    12541 aacagagggg atacctattg tatctactat attagacgga ataaacttag gtgcagcaat
    12601 taaggaatta ctagacgaac atgacccatt actaaaaaaa gaattagaag ctaaggtggg
    12661 tgttttagca ataaatatgt cattatctat agctgcaact gtagcttcaa ttgttggaat
    12721 aggtgctgaa gttactattt tcttattacc tatagctggt atatctgcag gaataccttc
    12781 attagttaat aatgaattaa tattgcatga taaggcaact tcagtggtaa actattttaa
    12841 tcatttgtct gaatctaaaa aatatggccc tcttaaaaca gaagatgata aaattttagt
    12901 tcctattgat gatttagtaa tatcagaaat agattttaat aataattcga taaaactagg
    12961 aacatgtaat atattagcaa tggagggggg atcaggacac acagtgactg gtaatataga
    13021 tcactttttc tcatctccat ctataagttc tcatattcct tcattatcaa tttattctgc
    13081 aataggtata gaaacagaaa atctagattt ttcaaaaaaa ataatgatgt tacctaatgc
    13141 tccttcaaga gtgttttggt gggaaactgg agcagttcca ggtttaagat cattggaaaa
    13201 tgacggaact agattacttg attcaataag agatttatac ccaggtaaat tttactggag
    13261 attctatgct tttttcgatt atgcaataac tacattaaaa ccagtttatg aagacactaa
    13321 tattaaaatt aaactagata aagatactag aaacttcata atgccaacta taactactaa
    13381 cgaaattaga aacaaattat cttattcatt tgatggagca ggaggaactt actctttatt
    13441 attatcttca tatccaatat caacgaatat aaatttatct aaagatgatt tatggatatt
    13501 taatattgat aatgaagtaa gagaaatatc tatagaaaat ggtactatta aaaaaggaaa
    13561 gttaataaaa gatgttttaa gtaaaattga tataaataaa aataaactta ttataggcaa
    13621 tcaaacaata gatttttcag gcgatataga taataaagat agatatatat tcttgacttg
    13681 tgagttagat gataaaatta gtttaataat agaaataaat cttgttgcaa aatcttatag
    13741 tttgttattg tctggggata aaaattattt gatatccaat ttatctaata ctattgagaa
    13801 aatcaatact ttaggcctag atagtaaaaa tatagcgtac aattacactg atgaatctaa
    13861 taataaatat tttggagcta tatctaaaac aagtcaaaaa agcataatac attataaaaa
    13921 agacagtaaa aatatattag aattttataa tgacagtaca ttagaattta acagtaaaga
    13981 ttttattgct gaagatataa atgtatttat gaaagatgat attaatacta taacaggaaa
    14041 atactatgtt gataataata ctgataaaag tatagatttc tctatttctt tagttagtaa
    14101 aaatcaagta aaagtaaatg gattatattt aaatgaatcc gtatactcat cttaccttga
    14161 ttttgtgaaa aattcagatg gacaccataa tacttctaat tttatgaatt tatttttgga
    14221 caatataagt ttctggaaat tgtttgggtt tgaaaatata aattttgtaa tcgataaata
    14281 ctttaccctt gttggtaaaa ctaatcttgg atatgtagaa tttatttgtg acaataataa
    14341 aaatatagat atatattttg gtgaatggaa aacatcgtca tctaaaagca ctatatttag
    14401 cggaaatggt agaaatgttg tagtagagcc tatatataat cctgatacgg gtgaagatat
    14461 atctacttca ctagattttt cctatgaacc tctctatgga atagatagat atatcaataa
    14521 agtattgata gcacctgatt tatatacaag tttaataaat attaatacca attattattc
    14581 aaatgagtac taccctgaga ttatagttct taacccaaat acattccaca aaaaagtaaa
    14641 tataaattta gatagttctt cttttgagta taaatggtct acagaaggaa gtgactttat
    14701 tttagttaga tacttagaag aaagtaataa aaaaatatta caaaaaataa gaatcaaagg
    14761 tatcttatct aatactcaat catttaataa aatgagtata gattttaaag atattaaaaa
    14821 actatcatta ggatatataa tgagtaattt taaatcattt aattctgaaa atgaattaga
    14881 tagagatcat ttaggattta aaataataga taataaaact tattactatg atgaagatag
    14941 taaattagtt aaaggattaa tcaatataaa taattcatta ttctattttg atcctataga
    15001 atttaactta gtaactggat ggcaaactat caatggtaaa aaatattatt ttgatataaa
    15061 tactggagca gctttaacta gttataaaat tattaatggt aaacactttt attttaataa
    15121 tgatggtgtg atgcagttgg gagtatttaa aggacctgat ggatttgaat attttgcacc
    15181 tgccaatact caaaataata acatagaagg tcaggctata gtttatcaaa gtaaattctt
    15241 aactttgaat ggcaaaaaat attattttga taatgactca aaagcagtca ctggatggag
    15301 aattattaac aatgagaaat attactttaa tcctaataat gctattgctg cagtcggatt
    15361 gcaagtaatt gacaataata agtattattt caatcctgac actgctatca tctcaaaagg
    15421 ttggcagact gttaatggta gtagatacta ctttgatact gataccgcta ttgcctttaa
    15481 tggttataaa actattgatg gtaaacactt ttattttgat agtgattgtg tagtgaaaat
    15541 aggtgtgttt agtacctcta atggatttga atattttgca cctgctaata cttataataa
    15601 taacatagaa ggtcaggcta tagtttatca aagtaaattc ttaactttga atggtaaaaa
    15661 atattacttt gataataact caaaagcagt taccggatgg caaactattg atagtaaaaa
    15721 atattacttt aatactaaca ctgctgaagc agctactgga tggcaaacta ttgatggtaa
    15781 aaaatattac tttaatacta acactgctga agcagctact ggatggcaaa ctattgatgg
    15841 taaaaaatat tactttaata ctaacactgc tatagcttca actggttata caattattaa
    15901 tggtaaacat ttttatttta atactgatgg tattatgcag ataggagtgt ttaaaggacc
    15961 taatggattt gaatattttg cacctgctaa tacggatgct aacaacatag aaggtcaagc
    16021 tatactttac caaaatgaat tcttaacttt gaatggtaaa aaatattact ttggtagtga
    16081 ctcaaaagca gttactggat ggagaattat taacaataag aaatattact ttaatcctaa
    16141 taatgctatt gctgcaattc atctatgcac tataaataat gacaagtatt actttagtta
    16201 tgatggaatt cttcaaaatg gatatattac tattgaaaga aataatttct attttgatgc
    16261 taataatgaa tctaaaatgg taacaggagt atttaaagga cctaatggat ttgagtattt
    16321 tgcacctgct aatactcaca ataataacat agaaggtcag gctatagttt accagaacaa
    16381 attcttaact ttgaatggca aaaaatatta ttttgataat gactcaaaag cagttactgg
    16441 atggcaaacc attgatggta aaaaatatta ctttaatctt aacactgctg aagcagctac
    16501 tggatggcaa actattgatg gtaaaaaata ttactttaat cttaacactg ctgaagcagc
    16561 tactggatgg caaactattg atggtaaaaa atattacttt aatactaaca ctttcatagc
    16621 ctcaactggt tatacaagta ttaatggtaa acatttttat tttaatactg atggtattat
    16681 gcagatagga gtgtttaaag gacctaatgg atttgaatac tttgcacctg ctaatactca
    16741 taataataac atagaaggtc aagctatact ttaccaaaat aaattcttaa ctttgaatgg
    16801 taaaaaatat tactttggta gtgactcaaa agcagttacc ggattgcgaa ctattgatgg
    16861 taaaaaatat tactttaata ctaacactgc tgttgcagtt actggatggc aaactattaa
    16921 tggtaaaaaa tactacttta atactaacac ttctatagct tcaactggtt atacaattat
    16981 tagtggtaaa catttttatt ttaatactga tggtattatg cagataggag tgtttaaagg
    17041 acctgatgga tttgaatact ttgcacctgc taatacagat gctaacaata tagaaggtca
    17101 agctatacgt tatcaaaata gattcctata tttacatgac aatatatatt attttggtaa
    17161 taattcaaaa gcggctactg gttgggtaac tattgatggt aatagatatt acttcgagcc
    17221 taatacagct atgggtgcga atggttataa aactattgat aataaaaatt tttactttag
    17281 aaatggttta cctcagatag gggtgtttaa agggtctaat ggatttgaat actttgcacc
    17341 tgctaatacg gatgctaaca atatagaagg tcaagctata cgttatcaaa atagattcct
    17401 acatttactt ggaaaaatat attactttgg taataattca aaagcagtta ctggatggca
    17461 aactattaat ggtaaagtat attactttat gcctgatact gctatggctg cagctggtgg
    17521 actttttgag attgatggtg ttatatattt ctttggtgtt gatggagtaa aagcccctgg
    17581 gatatatggc taaaatatat gtttgataaa aaattattcc tgtgctacta agaaattatt
    17641 tttatataat aaatattgag atttaattaa aagtcatgtg ttattgtaat acatgacttt
    17701 tagttaaaat ttttctatca tttaataatc tattattctt gactatttta taataaaatt
    17761 catatatgga aatattaata ctaaataatt aatagttgat aaaaaataga taatatgcta
    17821 aaagcaaaaa ctaatttaga gccttgtaac tgtttatttg caattataaa aacatcttta
    17881 aacatattga ctataatata aaatattaac tataatacaa aacaatatta attaattttc
    17941 tctacagcta tccctggtat agttatattt ccacccatag ttgattcata agttatagtt
    18001 ccagcactta tacctcttat agttatgtaa tcatcctcta atactcttga aggagttatt
    18061 gattttttat aactacatag tacaacctta tcataatttc cagacacagc taatcttatt
    18121 tgcacctcat caccatcttc aataacttga ataaccttac cttcaaattt tacctttttg
    18181 tacttataat catcaggtgt tctagctaat tggtcataag taataccagt atcatatcct
    18241 ttcttctcct cttcttcttt tttgcgttgt tcttcagcct ttttagcttc ttcagctttt
    18301 ttagcttctt cagctttacg ttgattttca gcttcaatag ctttcttttc gtcgtctttc
    18361 attttgaacc atggttcagc atcagacaat ttgctattta aagtttctat ttctgttttt
    18421 tgagaatcta taactgatgt ttggttagaa atgacctcct catggtcttc agaacaagct
    18481 ggtgaggata tattgccaaa aaacacgcct aaaataaatg ccagtagagc aatacccttt
    18541 gtgctcttaa agaatttaat tattttctta gagcttcctt ttccttcatt actaaattcg
    18601 ttaccatcat tttttttaga aaacataata caatctccct tttattagat atattttatt
    18661 ttaaaagtta aaaacttaaa ataactagtt gactaaaaat tatatattca tattttccta
    18721 aaatatattt cccctaagca taatttta
//
LOCUS       DBGJFU010000078        18612 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.078, whole genome shotgun sequence.
ACCESSION   DBGJFU010000078 DBGJFU010000000
VERSION     DBGJFU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.078"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(227..907)
                     /locus_tag="ACOIAS_002468"
     CDS             complement(227..907)
                     /locus_tag="ACOIAS_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702172.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(957..2972)
                     /locus_tag="ACOIAS_002469"
     CDS             complement(957..2972)
                     /locus_tag="ACOIAS_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2702173.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(3044..3721)
                     /locus_tag="ACOIAS_002470"
     CDS             complement(3044..3721)
                     /locus_tag="ACOIAS_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702174.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(4301..4384)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4617..5459)
                     /locus_tag="ACOIAS_002471"
     CDS             complement(4617..5459)
                     /locus_tag="ACOIAS_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2702175.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(5711..7495)
                     /gene="pepF"
                     /locus_tag="ACOIAS_002472"
     CDS             complement(5711..7495)
                     /gene="pepF"
                     /locus_tag="ACOIAS_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGZ2702176.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLVHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(7561..8226)
                     /locus_tag="ACOIAS_002473"
     CDS             complement(7561..8226)
                     /locus_tag="ACOIAS_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGZ2702177.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(8377..8538)
                     /locus_tag="ACOIAS_002474"
     CDS             complement(8377..8538)
                     /locus_tag="ACOIAS_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HGZ2702178.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(8616..9125)
                     /locus_tag="ACOIAS_002475"
     CDS             complement(8616..9125)
                     /locus_tag="ACOIAS_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HGZ2702179.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9331..9549
                     /locus_tag="ACOIAS_002476"
     CDS             9331..9549
                     /locus_tag="ACOIAS_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2702180.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9567..11513
                     /gene="feoB"
                     /locus_tag="ACOIAS_002477"
     CDS             9567..11513
                     /gene="feoB"
                     /locus_tag="ACOIAS_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HGZ2702181.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(11692..12747)
                     /locus_tag="ACOIAS_002478"
     CDS             complement(11692..12747)
                     /locus_tag="ACOIAS_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2702182.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(12795..13595)
                     /locus_tag="ACOIAS_002479"
     CDS             complement(12795..13595)
                     /locus_tag="ACOIAS_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2702183.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(13588..14379)
                     /locus_tag="ACOIAS_002480"
     CDS             complement(13588..14379)
                     /locus_tag="ACOIAS_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2702184.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(14419..14841)
                     /locus_tag="ACOIAS_002481"
     CDS             complement(14419..14841)
                     /locus_tag="ACOIAS_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2702185.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(14857..15336)
                     /locus_tag="ACOIAS_002482"
     CDS             complement(14857..15336)
                     /locus_tag="ACOIAS_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGZ2702186.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(15468..18224)
                     /locus_tag="ACOIAS_002483"
     CDS             complement(15468..18224)
                     /locus_tag="ACOIAS_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2702187.1"
                     /translation="MRDEILSFVNECVLFNSFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGIAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         6112 a         3172 c         2038 g         7290 t
ORIGIN      
        1 tatagattta gatatacaat ttatccatct tttcaaatat aatctataaa ataatagttc
       61 cattacgcat ttttttatat aataaaactg actgtaattt aactggcaaa gtctagaaga
      121 ataggattta tcaattaaat tacagtcagc tattttataa taatttagtt tagatatcgt
      181 tagtatttca catttataac aattattaat gatattaata tatttattat tccttattaa
      241 aataatatcc tgctcctctt attgttataa ttttctgagg atttttataa tctttctcta
      301 ttttctttct taatccattc actgcaacta ccacactata gttatcctct aagtattcat
      361 tcatccatac atgttcatat atctctttag tagtcaaagt cttttcagga ttctctataa
      421 aaaagtttaa caacttattt tctgttggag tcaattttat tattttatct tctaacttta
      481 ttactccact agttctatta tattcctcta attttgtatt attttcatta ttatttacat
      541 atgagttcat tcttcttaaa atagctttta cttttgcgta taatatttct aaatttagag
      601 gctttattaa gtaatcgtct ccaccagaat ttaaagcatt taaaatgctt tggtcttcat
      661 ttatataagt catataaaca attggacaag tagttacatt tctaattttt ttacaaagag
      721 tccatccatc accatcactt agaattattt ctaaaaatat taaatcatat ttattgctaa
      781 atatttttcc aatagcttct tcacaattat atgctaaatc tatacttatt cctttttcct
      841 ccagaaatcc ttttagcttc tggcaattat ccttgtctcc atctattatc aatatcttag
      901 tttgcattat aatcactccc aaatatcatt attcttttat attaaataag ctattattat
      961 aaaaaacata gacttaattt tattgtaaaa acagttcctt ttcctagttc tgaatctaca
     1021 aatatttctc caccatgctc tcttataatg gatttgacaa tatatagccc taacccttgc
     1081 ccttttggct cattattgtc tctttctgta aagaatctgt caaatacctt tggtatattt
     1141 tctggaggta taccttttcc attatcagaa attttaataa ttgcaaatcc atcttcttta
     1201 tccattgata aaattatctt tccatcaaat ggagtaaagg tcaaagcatt ataaattaaa
     1261 ttctcaaatg ctcttgtaag catctcttta tttatctttg catatatttt tttctccaat
     1321 acataagttt caaagtttac accacatgct tgtgtatctg gcaaattatt ttcatatatc
     1381 tctttaataa aattattcag acatattttt tcactaatac caatgctttt atcttgtgat
     1441 gtaaattctt gcaaactacg tactctattt tgtacttcat ttgacttttg atttatagca
     1501 taaagatagg ttcttgtctc ttcatctaca tgaatattac caacctttat caaatctata
     1561 aatgcttgta tagccgaaac aggtgccttt aaatcatgtg ctacatctgc caagaatctt
     1621 ttacgttcat ttaacaatgt tgtaagttca ttagttctca cttcaacttc atgttcaaga
     1681 ttccttgtaa gtttttcatt ctcttttatt attattctat tcctgtttat catcataaca
     1741 gaaaataaaa taacaattac aaacccacaa tattctgttt gccaccctgt aaatataggc
     1801 tcaaatctat tactcgtaac tgcatcaaat aatatgctaa ttccaaaaac agtgtttact
     1861 gaaactaaaa tataagaact tatatgctca gagcatgtct ttttatatat tgaaaccagc
     1921 gaagacatta atatatatat aaatacaata tatttatata tgtctattaa cttcccatat
     1981 atgttgacaa agtacatatc atatggaaga agaataattg gtatcactat agatatgata
     2041 ctcattgtaa ttcctaatgg taaaattaaa aatctataca ctttattttc ttctattttg
     2101 gatatacttc catttataca aataatacat aaaactacca taaaatatga caaatcttca
     2161 accgcatatg taaagcttac caaaggcagt cctaacaaat gtacaaaggg ataggataca
     2221 tggatggcaa agaaaacaca catacatcca aaatataagt aaattttact tttgtttgaa
     2281 agaaaccata cagacaatga aaatatagct attgcaaaag ttgtgaagca aattatacta
     2341 taaaaaagta atcgataaaa catcatatta cttatgtcct ttgtttctcc taatgcaggc
     2401 ggataataca aaccactata ataatgtgaa aaattcgata cattgattat aatttctgta
     2461 tttttctttg caataaaact tactactgaa tttttaattt tgggtctata tttttctgtt
     2521 cctacatctc ctaatttaga aaccatttct ccatttatcc atacttcact tgcggaaaaa
     2581 atctcaggaa gctctaaact tagtagctta tccttgcctt cattttttat tctcatacga
     2641 taagtagcat tcccatatgg tgactgagtc ccttttctca tagaaaaatc tggatattga
     2701 cctataaaaa catattctgg ttttatatct tgctctgtaa aatcattcgg tttaaatatt
     2761 ttatgattat aaaactccca gtcatctatc aaaaagacaa gtttaccttt agtgaaagta
     2821 tcattatcta tagttaaaat tccattactt gctctaatag acttatatgt atatttatta
     2881 tcatatttat acagaagatt taatcctgtt attgccagca gagagactat caatacaatg
     2941 agagttcctt ttagtatatt atactttttc attgtatcaa ccacatctct ttcatagtag
     3001 tattaaataa aacaagaact atttattaat tacagtatct cacttatggt acaaacttat
     3061 atccttttcc ccatacggtt tgaatgtatt gatgctccct gcttaaagaa ttaatttttt
     3121 ttctcatctg tgcaatatga acctgcactg tatgttgatc tccaatatca ggcattttcc
     3181 aaacttggtt atatatctct atctttgaaa atacttgttc tttgttctca gctagaaaca
     3241 ataatatttc aaattccata gtagttaaat ctatactttc tccatcaaaa gttacttttc
     3301 ttgaatttac atttattact aacgctgaaa actttaacac attaggaact gatgtaatat
     3361 ttttgtactg cctcatacga gcatatattc taagttcaag ttcttttaaa ctataaggtt
     3421 ttgttatata atcatccccc ccagctaaaa atccccgaac tttttcttct tcatgctcat
     3481 aattagataa aaatattata ggtatgttaa ccttttcttt tatatcattg cacattttat
     3541 atccatctat atctggcaat gaaatatcca aaattataca gtctaaaact atactctcta
     3601 ctatatccat agcatcttta gcagtctctg atgtatatac attaaaacct atattagaaa
     3661 aataaatttg atttatgttt aaaatatcaa catcatcatc tacaaccagt aaattataca
     3721 ttacaccact ccattcaaag gtattttaat tataattcca agttttatag ttattacaaa
     3781 ttataatgtt ttattttcat atataactgg tattattatt ttataatatc tttacaaact
     3841 agactataat acttaagtat ttttgaataa ttcttgaata ttgtattaaa cattaagatt
     3901 cattattaaa tactaattaa agtctccgat tgttggagaa tggagcttaa agtcattttc
     3961 acgtataaat ttattatttt tctagaaact tgtctattgg ctagtttgag tattgattta
     4021 tatgatttat atggttataa tataaaataa attgtattat tttggtaaat atattgttac
     4081 aaaaggttta tattctgcat ctcaatgttt ataaaaactt tattttctta ttataatact
     4141 attattcttt taaaaaaatt tcccttttcc aacaaatggc tactttaaaa gccaaaattt
     4201 tacctatcaa taaaaatctt aacgactaat actcttattt tcaaattttg caacattttt
     4261 gttaatttta aaacaaaaaa agactatgca atataaaaat tatattcata gccggttgca
     4321 ccactaactc catatatctc tagatgccca ataataagat atacttctca gtttctaaaa
     4381 tatttttatt aaaaatcttt tatatatttt cataaatttc ttttttattc taacattgta
     4441 atattatttt gtcaatattt ccatattctt agagataaga tactaaaata cctattttat
     4501 ctaaagatat ttatatttct acattcaata atagacaata ccctgcaagt taaagcatca
     4561 tttttatgag cttatctcac agggtattat atgtttactt tatattataa ctcttgttat
     4621 tgattaactt taataaatcc tttttctgaa acaagtgctt gtccttcatc acttaatata
     4681 taatctatta aagctttacc tttttctgac aaaccatctt ctttattaac taatataaat
     4741 ggtcttgata ttggatattt attagctatt acatttgcat cagttgcttc aacatcattt
     4801 atttttaaac tatttacttt ctcatcaaca tatccaaatg atatatatcc tatagcattt
     4861 tcatttcctt caactgtagt ttttatattt cctgaaccat cacttatttg tgcatcttta
     4921 gttagttctt ctgaatcaaa ccctacaatt tcttggaatc catctctagt tcctgaacct
     4981 tcttctcttg atactattac tattggtgca tcatttcctc ctacttcttt ccagttagta
     5041 atctttcctg tatatatacc tttaacttgg tccattgtca gattttttac tgagtttgca
     5101 gtattagtta tcaatgctat accatctatt gctattttag tttcctttaa tccagcactt
     5161 ttttcttcat cttttaaatc tcttgaactc attcctattt cagatgttcc ttctatagca
     5221 ttttttatac ctccagaaga accaatctgt tgtacttcta cactaacacc ttgatttttt
     5281 tcttgaaact tttctccgat agtttccata agaggaccta ttgaagttga acctgaaaca
     5341 gaaactttgc ttgaatctcc tgaatcacct gaacctcctg agttagaact acatcctact
     5401 gctaaagctc caactaacat tgtactagct aaaagaacgc ctattttctt tttaaacatt
     5461 ttacttaccc ccgcttatat tttaattttt tgttgtcatt tttctatatt ttaataatac
     5521 agattctttg ttaaatgaat attagagcag ggttaagtaa atgtaaatat tgttaaatac
     5581 atgtaaaaac ttaattttat atgatttatt cttaattaga ttaacttttt aaagttactt
     5641 taggcagata taaacataat acaactcata ttatcagaag tcttcattga agctatcaac
     5701 ctatcacatc ctataattct ttttctagtt tattaactaa ttcagcaaat acatttaagg
     5761 cttcctctat agattctttt ttcgtcatat ctacaccagc agattttaat tgttctagtg
     5821 ggtattctga ccctccactt tttaagaagt ttatatatct atctactgca gtacttcctt
     5881 ctgaaagtat ctgttgactt aaagcacttg cagcagaaaa ccctgtggca tacttatata
     5941 catagaagtt tgaataaaaa tgaggtattc tcgcccattc tattcctatt tgtttatcta
     6001 ctattgcaga ttttccgtaa tacttttcat ttaatttgta gtatatatta gtaaattcat
     6061 cagcagtaag tggttctcca ctctctaccc tttgatgtac aattttttca aattcagcaa
     6121 acatagtttg tctatgaact gtagttctaa attgttctaa ataataattt aataagtata
     6181 tagtttcatc cttagactta gaattttcta ataaataatt tataagaagt aattcattta
     6241 atgtagatgc aacttctgca acgaatatct tatagtccga atataaaaat ggttgacttg
     6301 ttcttgaata atgactgtga acagagtgcc ctaattcatg aactaaagtg aatagagaat
     6361 taaggtcatt tttatagctc attaatatat aaggatgtga atcataactt ccccaagaat
     6421 aagctcctcc ttttttacct tcattatcat atacatcaat ccacccttca tcaaaagcac
     6481 tttttatgac cttcaagtat tcttctccta aaggtttaag tgcctctaat atgatatcct
     6541 gtgctttatc atatggtata tccatgtcaa atttatctgt caaaggaaca tacaaatcat
     6601 acatgtgtat ttcattcaat cctaaaaatt tctttttaag ctcaacatat ttatttagat
     6661 taggaatact tttatctata gctgatataa gattattgta aacatctaaa cttacatcat
     6721 ctgaaaataa agacgcttct attgctgaat tatactttct agtttttgcg taaaatatct
     6781 ctgattttat tccaccataa agcgttgagg caaatgtatt tttatatttc tcatatgttg
     6841 aaaattcacc ttcaaaagcg tcttttctaa ctctactatt cttacttttt ataaataaag
     6901 agtagtttga atgagaaagc ttaaccttat tcccctcctc atcttctatc tcaggaaatt
     6961 ccatatctgc atatgctaac atatcgtata cattttctgg aattgtcgat aattctgatg
     7021 tagctgcaag tatttcttcc tctcgttcac tcaaagtatg tggtttatct ctcaatatat
     7081 cctttatata ttttgtataa aaggatagct tctcatcttt taagtattca cttaacttat
     7141 tctcttctat cgccagtatt tctggaacta tataagaagt agccacacct aaatcagtag
     7201 ataacatttc tgtcttagtg gctcttcctt gattatcatt aattctagta tcttcatgtt
     7261 gtttcatatg agtatataca tataaatttt gaacaatcct agatgcattt tcagagatat
     7321 ttaatacttt atataagttc tccttactct cagaaagctt ccctttatat tcttttactt
     7381 cttggataag attttttact ttacttatgt ctttttcaat ttcctcttta ctggagtaca
     7441 tcttatctat cttccatttg tacttttctt ctattgtact tctatcagtt cccatatcat
     7501 acacctctat tttaatttgt tttatatttt attcacttta caaaatatat tgtatatatt
     7561 ttagtccaaa taatctacat ttattactcc ttctatcata tttatttctt tcataacatc
     7621 gtgtaagtgt attctcttag cataagatac aaaaaaatta accttccagt attcttcatt
     7681 acgacattct atttccatat ttcttacgat aagttgattt ttttcaaatt tatcatataa
     7741 aaaaggtata gagtccaaat tgcttaattt taatatcaat acattctttt cttggctaaa
     7801 taaaaacgta ttgattatcc taagcacaaa taatgctatc attacaaata atccacctag
     7861 aattgccaat tcataataac catatccaac tgctatacca atacatgcag ttgcccacat
     7921 cccagcagct gtagtaagcc cttttacccc acctttattt tttaaaattg ctcctgcacc
     7981 taaaaaacca attccagata atacttgagc aggcattctt gctgggtcag catttgtata
     8041 caaattataa tcaaagaata attgaatcga agttattgaa acaatacaag aacctattgc
     8101 aattagtata tgtgtcctaa atcctgcaaa ctgtcttatt ttttctctct ctaagcctat
     8161 tattcctcct attattagtg ctaatagtaa ccttattgct atctccttac ttcctatatc
     8221 aatcataata taaaaccctc ctgacttaat tacaacttat gtcctacaga taatttacac
     8281 atcagaaata ctattttata tcttaatatt attattaata tatgtaaaat atagccatct
     8341 gttaattttt gatacaaaaa aacccacaac tgtgggctat ttgcttaggt acatgtattt
     8401 atttattaat atatctattt tcttacttga ttctactaac tcttcttgaa atatttctgg
     8461 atattctatg tatgtattta tctcttccct aactgcttca atctcatttt ttaacgcttc
     8521 taattcattg ttcgccataa taatcaccac tattacaaat ttgtatattt ttatatacat
     8581 tttatcaaac aatattatat tttgtcaata ttatgtcaat aaaacttact ataaaaatgc
     8641 tttaatttag gtattgtata cctggatact aagcctacaa acataccagt tactaatgat
     8701 gccattaaca taaatggtaa gtaccctata gcaagtatat tttctatgat taaacttgca
     8761 actattaatt gacctatatt atgccctatt gcacctataa tgcttatccc tattagtgat
     8821 aaatctttta ttttactcgc taaatacata aataaaagac tcagataagc tcctcctata
     8881 ctaaatagta gatatgataa agggcctgca aatattgatg acaaaattac cctgatagta
     8941 acaattataa cagtatcttt gacacctaac aacattaatg aaattaaagt tataatattg
     9001 ctcaaaccca attttgcccc tggaaatata actataagag gatttggtat atatgtctct
     9061 aatacatata aagctaaact ataccctatc ataagaccta aaaatgtcat ttttctagtt
     9121 ttcattaagt taactcctcc aaattttatc tatcatctta cattataata caaaatatat
     9181 aaaaacacct aatttagact aaaatatttt taatctattt agaataaata ttagttattt
     9241 caatgtgcaa ttacctctat aaatcttgac tttttgtatc atacttgtta caattttaat
     9301 gataatgaat ttcattagga ggtgttaata atgactgttt acgatttaaa aataggagaa
     9361 aaaggattta ttaatagtat agatggagat attaaattat ctaaaagatt gcttgcactt
     9421 ggatgtataa atagtactga aatagaagtt aaaaaggttg ccccacttgg agacccaatt
     9481 ataattagat ttagaggctt tgaccttgcg attagaaaat ctgatgctaa aaatatttct
     9541 ttacaataat agaggaggag ctacccgtga ttaatgtagc attattagga aacccaaatg
     9601 tcggtaaaac tactgttttt aatctactta caggttcaaa tcaatatgta ggtaactggc
     9661 ctggtgttac tatagaaaaa aaagaaggat ttcttggaaa agagataaag gttgtcgatt
     9721 tacctggtat atatgcaatg gatactttct caaatgaaga aaaggtttct aaatcatatt
     9781 tagaaaatga agatgttgat gtaatagtaa atgtagttga tgcttctaat ttatcaagaa
     9841 acctttactt aacaactcaa cttatgcaat ttaacaagcc tatagttata ttgcttaata
     9901 tgttagacat tgcagaatct aaaggagtta atatagatgc caaaaaatta agtgaagaac
     9961 ttggagtaat tgttgttcca attatagcaa aaaagaaaaa tggtgttgat aggatagaag
    10021 aagagattaa gggagctacg caaaaagctt ttatatatca gaaaaacttt ggaagtgaaa
    10081 cagaaacata taaaaaatta gaatccattt tatcgagatg tacaaaaact acatatacac
    10141 aaaagaaatc tatcagtgat aagatagaca atatagtctt gaatcctata ttagcatatc
    10201 cgatatttat tggtgtattg ttcctcttat ttaaatttac ctttgactgg gtcggaggac
    10261 cactacaaga gggatttgct agtcttatag aggcttatat ttctgcacct gttagtgata
    10321 tgttatctaa ctctagccca tggtttaaat cacttatagt tgatggaata attggaggtg
    10381 ttggtggtac tttaccattc ttccctttaa tatttacatt attctttggt atatcgttgt
    10441 ttgaagatag tggatacatg tcaagaacag catttttaat ggataaagtt atgagaaagg
    10501 ttggtctatc tggtaaagct tttataccta tagttatggg tatgggatgt tcttctccag
    10561 ctataatggc aactagaaca ttggaaagcg aaaaagatag aagagttaca gctcttattg
    10621 caccacttat gacttgtgga gcaaagctac ctatatatgc actatttgta gctatattct
    10681 ttccacataa tgctgcttta gtaactacat cactgtattt ggtgggtata gttatggcaa
    10741 ttgtagttgc attattctta aataaaacag cctttaaaac agaggctgaa ccatttatat
    10801 tagaattacc agaatataga atccctacta tagatgcact tattaaaaat acttggaata
    10861 aatcaaaagg tttcttgata agagttgtta ctgtcatgtt tgcaatgtca gtcgtaattt
    10921 ggggactttc ttcattcaat gtttttggat atacagaaga tataaatgta agcttcttgg
    10981 ctactctagg tcatattata tctcctatat tcaaacctct tggttttgat gattggagaa
    11041 cttcagttgc tattttaagt ggtcttggag caaaagaaat cgtagtaaac tctttaaata
    11101 tactatatgg taatctaact gttgtcttac caacaatatt taatggagtt actgcatata
    11161 cattcttgat atttactgct ttatatactc catgtatagc tgcacttgca actatgaaaa
    11221 aagaatatgg caataaaatg atgtttacat catttgcata tcaatttata ctagcatgga
    11281 taatggcttt tatagttaaa agtataggtg gagtactatt tatgggtaat tcaataatgg
    11341 aagctttgat aggtggaata attatagtcc ttgcactctt tattctgttt aataaattca
    11401 agtctacaaa aaacggaaat gtctgtagtg gatgttctat ttgttctgga tgtccaagtg
    11461 ctaattcttg ctcaagtaag gaagacagca ataaaaaaaa taatgcacac taatatataa
    11521 atattttagt ttaaaataag ataaaagtgg gtgtctcaaa attaactttt tagttcatga
    11581 ggcactcttt tttatataaa atcaaatata ttttcatcat ttcaacagta aaaaagagct
    11641 tatctagtaa aaaagaggct tatctctatt ttgagacaac ctcttttttt gttacttatt
    11701 cagtttcttg ctacctaact tcatgtggaa cctagtatct tttggaacac ttgggtctac
    11761 acctcttaat gtaggtattt ccccacatat tagttgtata ggtactataa aattaaatgt
    11821 ttgatagtaa ggattatctg tgatatcaat ctttaaattt ttatcatttt ctgtaacatc
    11881 tctacctata gcaaatacat tttccgtcca accagaaagt acatcaatca tcttagttac
    11941 tctaggttct tttcctgtat ctagtataaa tatagtagaa tcactattta tagcattata
    12001 tataccatgt ataaactctt caaactcata cccagttaca ggtattctca ttgtttccaa
    12061 taattttaat gctgcttcta atgtatctcc atatatatca gagtgaccta ttattcttat
    12121 ttctttagaa ttaactaact tttctttgtt tctttcaatc cattgtttag agagcttata
    12181 cactgcttca aatctattaa ttgcgtctaa tatcttattt atttcctcat tgtacttttc
    12241 actagatatt ataccttttt ctctagctat ttgaagacct aataacatta agtttaactt
    12301 tgtacaataa tatccttttg ttttagctcc agatttttct tcaccacaat ttactgttaa
    12361 tatataatca gaaatctcat ctattaaagc attcttacaa cctgccatag atgctatttt
    12421 acagccctta tcttcagcta acttcatagc gttgtatgta gaataactac tcccaccttg
    12481 agatacacct actactaggg tattttcatt atcaaacttg aatgtatctt cagttatcat
    12541 aaatggatac attttcacaa catctatatc taataaattt tgtaaatatg gttgcacctg
    12601 cactcctgag tgatagctag tacctgaacc tgttattata atctttttta gatttgttct
    12661 cttaacttca ttaaataagc tatctgcatt agatataatt tctctcattc taactggtgt
    12721 ttctaacata taatcttgta ttgtcatttt atacaccttc cttatctata tattcaataa
    12781 attataatta atttctataa caaacctata gcatgtaaag cgattcctgt tactatagtg
    12841 cctatagtta attttgttac acttaccttt ctctttaaca tgtagtatat tacaaatgca
    12901 aaggttaaag gtaacatatt aggtattatt ttatctagta tatcttgtaa aattacttct
    12961 gcaccattta agttgaattt cagaggagta gttatactca acatagttgc aaccattcct
    13021 cctacaacga ataaaccaac agttgtagcc atagacatta ttttctccat aagtccctct
    13081 ctttgtattc tttcaagaga atttacccct acttgataac catacttaag tccaaatatt
    13141 ctaagggcaa atgctggtat attgtataat agtaagaata atagtggtcc aaagatatta
    13201 ccttcttttg caagtgatac tcctactcct gctgctataa tcctaaatgt tccccagaat
    13261 aatgaatcac caattcctgc aagaggtccc ataagagctg ttttaacagc actaattgaa
    13321 ctttcatcaa attctggatt attagcattc tcttcttcca tagcggctgt tatacctaat
    13381 atagcaggaa ctaccactgt cgttgtatta aaaatctcca aatgcctttc tattgctttt
    13441 gcttgatttt ctttcttatt gtatagcttc tttattgcag gtaacattga ataacagtaa
    13501 cctaagtttt gcattctttc gtaattaaat gcaccttgaa gagcaaatga acgccaaaac
    13561 atacttctta attctttttt tgttaattta ttcaaagtca atctctcctt ccgttaaagt
    13621 attgattgct aattgaggat tttgattatt ttctttgtta ttgatataaa agtttagtac
    13681 aaatgctaca catactccaa ataaagctat agcagttata tctaatccac cataagatgc
    13741 taacaaaaac cctataaaga aaaatacaga catctttttt gaaagtaaca tatccataag
    13801 caatgcaaaa cctactgctg gcaacaatgt tcctgcaaca cccaatccgt ctaatataac
    13861 tttaggtatt gcatttatta atgctgtaac tttatcagca ccaatcataa ttgctaaaaa
    13921 ggttggaact gatgtactta aaaagaataa tattactggc aaccacatca ttatggtaac
    13981 tttcttaaaa tcagcctgtt tagcatagaa aactgccttt tgagaaaagt acgaatttat
    14041 tattctaact aatactccaa gagattgtgc taagactgct actggtactg ctactgctaa
    14101 agcaacttct gcaccattac ctgataatat agcaaaagct gtacctaata cacctcctgt
    14161 tactacatct ggaggagtag ctgcacctat tcctgcaatt cccatccaaa ttaattcaag
    14221 ctgtgcacca atcataattc ctgattttat gtctcctaaa actaatccta ctaacatacc
    14281 tgtaactatt ggcctatcaa gcatagattg accaaatata cgcccatcta gtataccaac
    14341 tcctgctata atagctaaca tagtagcttg tactaacata ttgaacctcc attttaactt
    14401 agtttaattt acattaaatt acattacatt tttgattttg ttttgatttt ctaataactc
    14461 atttacataa acaattccat tttttgcttc ctctaatcct gatttaacta ttagttttgt
    14521 atcctcctca actaataaaa gtatagataa aagcattggt aaatttactc ctgtcaataa
    14581 ttcaaatttg aacccttgta aaagtaagtt tgtacatatg tttgccacac ttccaccaaa
    14641 cacatctgtt aagacaacaa attcttcact atctttatta tttgataata tacttctaat
    14701 ttctttctca gcatcatctg ctgatttatc cgttgtcata caaaaatact caatattagt
    14761 taatgaacca attattaatt tacttgtgtc aacaagctct ctggacaatt ctccatgtgt
    14821 tgcaattaga aattttttca ttctctttct cctaaattat tttattaaat ctgatagttg
    14881 tttctttttg tctctaggta acattctaaa ttctatatca atccctaaat cacctatttg
    14941 tttaaagtta tttatatcat catcatcaac agcaactaaa tctgtaagtt tcttcttccc
    15001 ttcctcatat cttaatcctc ctacatttat ttcttttaat actcctcctg aatttttagc
    15061 tacaactaaa gcatctttgg tattttttac taacactaat acattgcttt tctttgaaga
    15121 agcaaatttt tctactattt ctccagattc ctctaccccc cttatatata aagttacatt
    15181 gctaggtttc gctaagttca gtgacatagc tttcatttgg tcattttttg cttcatcatt
    15241 tgcaactagt attacattta tacctagtga tgttgcccat ccatatgcaa cctgaccatg
    15301 tatcaatctg tcatcaattc tcatcaaaga taccattttc cccaaatcct ccatcataca
    15361 ttaaaatttt tattgtttat actctcattt atctataaaa gcaaaaagta tgccaaatac
    15421 tggcatactt ttttaaattt cattcactta ttttgtaatc ttgtatttta ctgtggtaga
    15481 ccatagataa tattataaat atatcctatc tctgaaattg gtatttgcac actataactc
    15541 ttttctagca cactaaatgc attttttatt agttttactt cactatgatg gtttttgatt
    15601 aacaaatcca tatcagtata ctcttcaatt tcagtttgtc taactaatct ttcaatcata
    15661 caacttaagt gaacatataa agaaatcttt ttatcatttg aaagtttttt cttttgaata
    15721 ttttctatag cattaattcc ttcttctatt ctctctatta tcttttttga atctaaaata
    15781 gttagactat ctattactct aattaatgaa aaattcttta caatagaatc atttatgttc
    15841 tttatttttt cattttctac tactctgcta aatattctaa aaacatcatc ttctcctttt
    15901 cctgatatta tatcctctaa taatataaaa tctaccccat tcacatttgg attggcagtt
    15961 cctactatag ccatcacatc atacatttca aaaactggtt ctttatctcc catagaaaca
    16021 agactatcat aatcataagg tattattttg acatcaacta ttccaattaa actttcggaa
    16081 agtagatgtt ggatttttat agcaatacct atccctgtaa aacaagatga tattatagca
    16141 tattctttat ttttttgagg ataatacaat tgtatattag gtttagtttc ttcatccata
    16201 atttctgcaa tctcttcaat gtttttacct tgcattaata aatttcctac ttctagtgca
    16261 gaaatagtgg atatttggtc tataaataac attgggctat ttatcttatt ctttaaatct
    16321 tcatatataa gattaagaga gcccatatca aataatacaa ttaaaccatt ctttatccta
    16381 tctccctcta gatatctaga catttgtttt gatacatcag caatagtagc atctataggc
    16441 atatcaatag ctgtaaatat atttttaccc aacatccgat tacatacatt cgccatacta
    16501 ctggcagtag catatccatg tgctataata gtcgcattaa cttgattaaa tttcattctt
    16561 ataattgttg atttaataaa tataatcatt atgattgtat ctacattatt aagatacata
    16621 tccaatctat tcgttattaa tgttgatatt ttacttatta tagagtattc tttgtaactc
    16681 tcttttttta aatatattag taatttattt tctaaagctt tatactcatc agacaattga
    16741 taagattcgt ccttcaaata aagataagac gaaaatgcca atacattatt cccatcatat
    16801 tttatattgt tattaacttc taaatttcta aatatatcct gcattatcga tattataagc
    16861 tccataagta cgttattatt ttcatcatta tttgaaaaga ctaaattgtc cattagtcta
    16921 atcattagat tagaactctt tttactaaat ttttcctttg ttatttcatt tttttctagt
    16981 ctatcaaagt aatctaaaca ttttatgaaa ctttgataga ttatatttct tttatttctt
    17041 ccatttgaca tttcaatact aaattgttta ggagaaaaga taatgctttt gcttttacca
    17101 ctttggaatt gaaatatatc tacattttct atataaagat ttctaggaag gtcttgaaga
    17161 gtaacaaaaa tatttccatt tcttctttga ttttttgcat aagctcgtcc acatgcatat
    17221 tttattacat ttttacattc acctatattc cccttgaatt ttatctttaa aatcgcattt
    17281 aatgtaattt gagttatctc tataggcaaa ccaagtacct tactttcttc ataaataaaa
    17341 tgttctacaa tctttctttt ttcaatagtc cctctttctt ctatattggg aacataaata
    17401 tttatgggta ttcttcttaa aaaggtttgt aaaaattcag atagactttc tgttgtagca
    17461 aatattaatc ttaccctagc ttttctaatt actccattct ctccaatcct agaaaacgta
    17521 cctgtatcca taaatgtaaa taacttttcc tgaccttcac tatttaatct atggacttca
    17581 tctaaaaata aaacaccacc atctgattgt tctattagac ctgctctaga ttcatttgct
    17641 ccagtatatg ctccttttac atgcccaaat aatagactag acaataactc caaattatta
    17701 taatactgcg cacagtttaa cacattgtat ggagaatttt tttctaatac attactgctt
    17761 acactatatt tgtatattat cttggctaaa aaagtttttc ctactccact ttcacctgtc
    17821 agcattattg taagtccgcc atttggataa agtacagcgg tttttatttg ttcgattact
    17881 tcttttaagc tattatcata tcctatgata ctattaaaca cagtctcttg ctttaataat
    17941 ttacactcat ttttaagttc atctaaattt tcataaatac ttttttttgt aggtcctact
    18001 acatcattta ttttattttt aggtaaaaaa ataacaggtc tactgtttat ctttataaga
    18061 ttatcagatt ttactaaatc atttaataat ctacttgatt gagttctact taaattaaac
    18121 ttttttacta tatcttctgt tattatgcca ctttcttttt taagttcatc aaagctgtta
    18181 aataatacac attcatttac aaaacttaaa atttcatccc tcatgttcaa cctccaatac
    18241 aaatatcaat aatactataa tattagtata tcatttatat atttatgaaa aagataatta
    18301 tattcctatc ttgattctct tttgtaaggt tgtaaaatca aactttttat aagtataaaa
    18361 tctagatttt ttaacataat ctcaaggttc ttatctttcc atatatctga catagaattt
    18421 atataagaat atatttattg tatttattat aataaaaagt aaaacattat aggtatttta
    18481 aaacttatta gcgaagaaaa tatttgtaac ttttattacc ttatgaatat tatttcaaat
    18541 ttaaaaaggc tataaataaa tttattttta aataaatcta cttataacct cacaataata
    18601 cttataaatt tt
//
LOCUS       DBGJFU010000079        18522 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.079, whole genome shotgun sequence.
ACCESSION   DBGJFU010000079 DBGJFU010000000
VERSION     DBGJFU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.079"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            140..1300
                     /locus_tag="ACOIAS_002484"
     CDS             140..1300
                     /locus_tag="ACOIAS_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HGZ2702188.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1643..1804
                     /locus_tag="ACOIAS_002485"
     CDS             1643..1804
                     /locus_tag="ACOIAS_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702189.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            1997..2626
                     /locus_tag="ACOIAS_002486"
     CDS             1997..2626
                     /locus_tag="ACOIAS_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGZ2702190.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMGGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2866..4125
                     /locus_tag="ACOIAS_002487"
     CDS             2866..4125
                     /locus_tag="ACOIAS_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HGZ2702191.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4396..5379
                     /locus_tag="ACOIAS_002488"
     CDS             4396..5379
                     /locus_tag="ACOIAS_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HGZ2702192.1"
                     /translation="MTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIKVIKH
                     NNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDE
                     LILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITK
                     ANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKM
                     LQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEV
                     EDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5625..6692
                     /locus_tag="ACOIAS_002489"
     CDS             5625..6692
                     /locus_tag="ACOIAS_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGZ2702193.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7226..9136
                     /locus_tag="ACOIAS_002490"
     CDS             7226..9136
                     /locus_tag="ACOIAS_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGZ2702194.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9199..9822)
                     /gene="ytaF"
                     /locus_tag="ACOIAS_002491"
     CDS             complement(9199..9822)
                     /gene="ytaF"
                     /locus_tag="ACOIAS_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HGZ2702195.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10010..10654
                     /locus_tag="ACOIAS_002492"
     CDS             10010..10654
                     /locus_tag="ACOIAS_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702196.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10868..11926
                     /locus_tag="ACOIAS_002493"
     CDS             10868..11926
                     /locus_tag="ACOIAS_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HGZ2702197.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12030..13214
                     /locus_tag="ACOIAS_002494"
     CDS             12030..13214
                     /locus_tag="ACOIAS_002494"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HGZ2702198.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13421..14134
                     /gene="rpsB"
                     /locus_tag="ACOIAS_002495"
     CDS             13421..14134
                     /gene="rpsB"
                     /locus_tag="ACOIAS_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGZ2702199.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14213..15124
                     /gene="tsf"
                     /locus_tag="ACOIAS_002496"
     CDS             14213..15124
                     /gene="tsf"
                     /locus_tag="ACOIAS_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGZ2702200.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15209..15913
                     /gene="pyrH"
                     /locus_tag="ACOIAS_002497"
     CDS             15209..15913
                     /gene="pyrH"
                     /locus_tag="ACOIAS_002497"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGZ2702201.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15939..16496
                     /gene="frr"
                     /locus_tag="ACOIAS_002498"
     CDS             15939..16496
                     /gene="frr"
                     /locus_tag="ACOIAS_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGZ2702202.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16499..16702
                     /locus_tag="ACOIAS_002499"
     CDS             16499..16702
                     /locus_tag="ACOIAS_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702203.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16731..17465
                     /locus_tag="ACOIAS_002500"
     CDS             16731..17465
                     /locus_tag="ACOIAS_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HGZ2702204.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17478..18257
                     /locus_tag="ACOIAS_002501"
     CDS             17478..18257
                     /locus_tag="ACOIAS_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGZ2702205.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7607 a         1853 c         3223 g         5839 t
ORIGIN      
        1 gtcaatagtt aggtatatat gtatttaatt agcatgatat agataaaagt atttatttta
       61 attgtctatt atctgaaaaa gtctttactt agaaatcata aaaatcaaat taattaggaa
      121 aagaaagggt gtgtataatg tgattgagac tggatttgta tattgtagtt tcttggttgt
      181 attcgtaggt gtaatttttt ttatggttga taaattgaaa tggaaggtat ttgatgttat
      241 taatccaatg cttttaatat acttaggttc agctatactt ggatcatttg gtttatttgc
      301 acagaatgaa gatgttggaa cgtatcaaaa cataataaca gtgaacttcc ttccattaat
      361 gttagtgttt ttgttaattc aatgtgattt aagaaaaata ttaaagatgg ggcctaaaat
      421 gttattatca tttttctgtg ctacatttac atttatgctt ggatttgtta tagcattcat
      481 aatttttaaa aattcaatgg gtgcagattc atggaaaatt cttggggcag catctggttc
      541 atggataggt ggctcatcaa atatgattgc agcagctaat gctctaggag ttgcagaaga
      601 aggacttagt tatgcaatac ttatgggttc tataggatat acagtatgga tgtctgtttg
      661 tctaatgtct gtgaaatttg gacctaaatt taacaaatgg acaaagtgcg atacaagttt
      721 tatagatgag gttacatcaa aacttgaaaa agaaaataaa gataatacag cacctacgtt
      781 tgtagaactt atgattttac tttctgttgg gtttggagtt gcgtcttttg cacaaattgt
      841 atccaagata cttccaactt caggtatact taatgaaact atgtgggtag tacttgttgc
      901 atctacagta tcaatattat tgggtatgac aaaaatatca aagcttaaag ggagtacact
      961 ggtaggaaat gtgtttatgt atttactcct tgcagctaca ggtagtaaag caaatttctt
     1021 tggattgtca gaagcacctg tttacataat gtttggattg gtagtagtcc ttgtacatgc
     1081 tatttcattt atagggcttg ctaaactatt taaacttgat ttatttactt gtgaagtagg
     1141 aagtatagct aatataggag gagtaactac agctccaatg attgctgggg ttcacaatcc
     1201 tgtattaata ccagtaggtg tacttatggg gcttctagga aatattatag ggacttattg
     1261 tgctcttatg gtaactcaaa tattacatat gttagcttaa tgaaaatatg gtataaaatt
     1321 aataatttaa ataagaaaag tcatttcaat ttaagcagaa gcttttactg aaatggcttt
     1381 ttttgtttga caagataatc tatttcaaga agatgttttg acatatttag tgtataatat
     1441 tgtaaaattt actaaaaacg agatatattt aatttttata ttgaatcgaa cgtctgtatg
     1501 atgtataata aatatacaga acgaatgttt gcggaacggg tgttttttga atttatttta
     1561 ataatttatg aatatatatc agaatgcata aatatattta aatataaaga ttaaaaagaa
     1621 tttaaaggag tgttgataac ttatgataat tccatcaaga agaatacaag atataacatt
     1681 aacaaattta aaaaatggtg aagtaacttt aattgaattg gatgaaatat acaagaagat
     1741 gggattttta tttgttgtta gtgaaggcaa attgaaaaag ataaaaaagg agaatcatca
     1801 ttagaaatga atgaaataat aaatattata atagtttata aaacctaact tatgtagaat
     1861 ataaatactt aagttaggtt tttttgtgat aaagtttata agtactcaat tttataagat
     1921 aaataatggt tttaagtgaa aagtaaataa gtttaaattt tagtaagtat tatattgcat
     1981 aagaggtgat attattatga aaatagtaac ctacaatatt cataaaggta tggattcaaa
     2041 taatagatta actctaacta aaatggggtt atatttaaaa caattggatt gtgatgtaat
     2101 ttgtttgcaa gaagtattat atcctcaatt tttagcttta aaagctgttt taaatatggg
     2161 tggtgtcttt gctacaaatg taaaaaaagt aaatatgata tatggtattt gtacatttac
     2221 aacatctaaa atgttaaaca gtaatcattt tttcttaact agcaaaaaag aacaacgggg
     2281 agctctttgt ataactatag atgcatatgg aagaattata aatgtaataa atactcactt
     2341 gggacttgat aggcaggaga gagcaaaaca attagacgaa ataatagact ataggaacag
     2401 attagtgggg atagttattt tatgtggtga ttttaatgag aaaaatgtct ttttaagtat
     2461 gtttaatgat atggctattt cactaaacaa gtcttactta ccaacatttg aaaaatcaaa
     2521 ctctagaata gattacattt ttgttaataa aaatactgaa ctaaaaggat acacagtaga
     2581 aaaaatttac ctgtcagacc attatcctgt aataggttac atttaaattt caatataaat
     2641 gaaattagtt gttataaaat aaacaatttt tttgttaaga aatagtaaaa gaaatataat
     2701 aaaaaatatc aaaaataaaa ctaattataa gacataaatt tacaataaag ttacatttga
     2761 ttaaattcct tgtttaacaa aagctattca aatataatct aaggtagttt tgaaatggga
     2821 actctattat gcaaatttaa gctagtgtta aaatgaggtg aagttatggg cagaagaaca
     2881 acaagagttt caagattagg taaagaaaaa aaacgaagtg ttttaattgt attggcagct
     2941 ttagttctaa taatgggtgg atttacttat ttttttaaca gtaaatttct gtacaatggg
     3001 aaaatcgcta aaaatgtata tatagaagga gtaaatgtat ctgatatgac taaagcagag
     3061 gcattaaaag ctataacaga taagtatact ccagaagact taaatcttgc ttatgatggg
     3121 aaaaaatata caatatcacc aaaagatatt gatttaaaat atgatacaga agatgttgta
     3181 aaagatgctt atgaaagtac aaaaaaaggg tcctattttc aaaacttaaa aaaatatatt
     3241 gatataagag tgaataaggc aaatatgaag ataaaagctg aatatgatga ggcaaaactt
     3301 agttccaaag tgtcttctat agcagatagt ataaatgtta agatgaaaaa tgcttctatt
     3361 agtgttggaa gtggtggact aagttataca gactctgttg taggtagaga atttgatttg
     3421 gcagcaaata aagagtctat atataatatg ataaaaaata aagagcataa aactttagaa
     3481 cttaaagtaa atcttcaaaa accagatata acaactgaac aagtaaaaac tgtaaattct
     3541 gttataggtc aatattcaac tacttattca caagctgtag agggtaggtc atataatgta
     3601 ggattatcag caagaaaaac tagtgatgta ttattaatgc caggtgaaga attttcatac
     3661 aataaactta caggacctag taataaagca aatggttaca aagatgcacc tgttatagtt
     3721 tatggtaagc tagaacaatc agcaggagga ggtgtttgtc aaacatcttc aacagtttat
     3781 aatgctgctc ttctttcggg tatggagata actcaagtta caaatcattc atctgcatct
     3841 acttatgtgc ctaaagggag agatgctact gtgagcgatg gaggattaaa cttaaagttt
     3901 aaaaatccat ataagcatcc tgtatatatt aaaaattatg caggtggagg aagtgtatca
     3961 tcagtaatct atggtaattc tggtgacaaa ccaaatatat ctatagaggt aaaacaaact
     4021 ggtcagaata aatattctac atatcgaata tttaaagatt caagtggaaa ggtcataaaa
     4081 aaagaacata tatcaaatag ttcttataaa gaattaaaaa aatagataat tttttagaga
     4141 aatatttgta atataaaagg gagtgtctca aaatgaactt tttagtttat gaggcactct
     4201 ttttctgtga aatcaaatat attttcatca tttcaacaat acaaaagaag cgtatctcta
     4261 ttttgagaca gccttttttg taaaaattaa cactattagt tcttgtttta atattacaat
     4321 cattaatata tagaaaatat tcctatatat tggtataatt gtaaatagat ttagttatat
     4381 aaaatgtatg aggaggtgac atggttgaat tttataggca atagatatga ggtagtaaat
     4441 tctgatgacg ttttagaaat taacaaaatc tataaagcta gagatgtttt ttacaggaaa
     4501 aatgtgttaa taaaagttat aaagcacaat aattatatct gtgaagattt tgtttcaaat
     4561 ttaattgatg aaagtactgc tttagatgag ataaactctc cttatatact aaaaataata
     4621 gatgttggga ttcactgtac agaagaaaca acattatatt atgttgtaag tgaagacttt
     4681 aatggtatcg gactagatga attaattcta ggtaattatc ttcatttaga agcaattgta
     4741 aacataatga tacaagtttt aaaagcttta gaaatgatac ataggaatca ttcatatcat
     4801 ggtagtttaa aatcgagtag tataatagta gatacagaat acaatataaa aatttgtgat
     4861 tttggaatta caaaagcaaa taatggggtt aatacaagaa gctatggaaa caggagatat
     4921 ttatgcccac atcaactatg tataaattat actgataaag aaagtgattt ttttgcaaca
     4981 ggtcttattc tatttgaatc tatatttaaa aaattgcctt ttggagaatc aaattcagaa
     5041 gaaaaaatgc tacaatctat tgataaggga ttagattgga atagtataaa agcaattaat
     5101 ggaaatactg aacttataaa tgttataaaa aggcttcttg gaagaaataa taagtatact
     5161 agagccaatg atattattat tgatttaagt aaagtaatgt atgaaaaagc atatatagaa
     5221 gttgaagatg agaaagaaga agagcagaaa caagacaaag ttatacaagt taaaaaagaa
     5281 tataaaagta aaatgagaaa aaaattacat aaaaaacttg ttgttgccgg tctagctata
     5341 gtcatgattt caatgattat tattagttcc ataatataaa aattgtatat taagtatttt
     5401 aaaatcaatg tctataaagg atatacatag aaattttagt gtagtcttaa attatgaaag
     5461 gaagtggtta ttatgttaac aaaaaataat gaggtattaa acgtctagga gtattttaac
     5521 aaatagccac ctgcttttac agtttagaat aaaaaagctg tggctaaagg tcacagcttt
     5581 ttgctacttt ttaaaggcag gagaatatta aaaaggagca aattatgagc aatataaaaa
     5641 taatagcaag taataaatgt tggattgaag atatagcaag gaaacaactt gaagatattt
     5701 caaagttaaa tggaatttta agagtagtag gattacctga cttacatgca ggtaaaatac
     5761 ctgtaggttt agcagttgag actaaaaata ttatttatcc acatataatt ggaaatgata
     5821 taggatgcgg aatgacttta tttaaaactg gggttctaaa aaagaaattt aaaaaagaca
     5881 aatggattaa gtcacttagt aagattaaag atttgtccga tatagaaata aaaaacacat
     5941 ataaagaaga atgtccaatt ttaaatttag gaactatagg gagtggaaat cattttgtag
     6001 aaattcaatg tataagtgaa atttataata aagaacaatt tgaacaatta aaattttcat
     6061 ctgatgatat tatgatgtta gttcactgtg ggtcaagaaa ttatggagaa gatattttaa
     6121 aaaaatttta tgataaagat ggacttgaag ttgaaagtga gaaagctata gattatatta
     6181 aaaaacatga taatgcttta atgtgggcag agcgtaatcg tgaagctatc agtaaaaaaa
     6241 ttatgcagtc aataggtaca tctggagatg ttgaaacaat attctctata aatcataatt
     6301 ttatagagaa aagggaagat aaatttatac atagaaaagg tgctgtttca agtgaaagag
     6361 gagctgtgat tataccaggt tcaagaggca gtctatcata tatagttatg cctactgaaa
     6421 atacagagat atctttatat tctctatcac atggagcagg tagaaaatgg tctcgttcag
     6481 tttgtaaatc tcgccttaaa agtaagtata ataaagatac tataaaacaa actaaattta
     6541 aaagtcagat tatatgtaat gatgtaaatt tattatttca agaagctcca gaggcatata
     6601 agaatataga gcagattatt gaaagtttga tagaatatga acttattcaa gtagtggcaa
     6661 ctatgaagcc attgattact tataaaggat aatttttaaa gttaaaaaag attaaaatta
     6721 tattataaat agctaatttt atggaacatt taaaagtgtt ttattattag ctattttttt
     6781 attttgtaga gacgataatt tatagagcta aattctttga tatataatga ttacaaagtt
     6841 agactgtatt ggaaaatgca catacatgaa ttgatgacaa tataagtgat tcccaaatat
     6901 aaatgaaagt ataagcttat tttcactata aaaattttca ctgtacaaaa acattaatga
     6961 cattattttt attgacataa atatataatt tatggaataa tattagaagc actgatataa
     7021 gtgtttaaat aagaatagaa aaggagaagt gttttatgaa aaaaaatact aacattaatg
     7081 agtccggagt taaacttctt aataaataag gagtttgata tataataact ccttatttat
     7141 taagttgttc gcaaaataac tattatcaaa gtaaatgata gggtgattta aaagacgata
     7201 acttataaat aaaggagaaa aaataatgaa tagtaataca tttgaaagat tacaactaaa
     7261 tgaagttaaa gaattaataa aaatacattg tgtaagttca ctaggaaaaa atttaataga
     7321 taaattaact cctagtggga atattggagt agtaagaagg aaattagcag aaaataaaga
     7381 agctagaaag ataattgaaa atagtaatca cataccactt gaaggattgt ttaatgcagg
     7441 ttctacaata gataaaattg aaaaaggaat gataatagaa cctgttgaac ttgtaaatat
     7501 tgaagatttt ttaagaggtt gtaggaagat gaaagctttt atgttagaaa aagaatttta
     7561 ttcacctaca ttaagttctt atgccttaaa tataacagaa tgtaaaagta tagaagatga
     7621 gataaattac tgtataaaat ctaataaagt tgattctaat gctagtaaag aattaaaaaa
     7681 gataagacga aatatagaaa ttacagaagg aaagataaaa gatagattaa ataaatttat
     7741 aacatcaact gtaaataaaa agtatataca agagtttata ataagtaaaa gaaatgatag
     7801 atatgtaata ccaattaaat cttcttataa aaatgaagtt aatggtacta tattagatac
     7861 atcatcaaaa ggaaatacgg tatttattga acctattagt gtttcaaatt taagtacaga
     7921 gttaactatg ttaaaagctg atgaaactat tgaagaatac aaaatattat cttatttaac
     7981 agaacttata tttgagaaga taagtcagat aaaattaaat atagagatac tttcagaata
     8041 cgatatggta tttgcaaaag ctaagtatag tcaaaaaata aaaggaatta ctccaaaaat
     8101 aaataataat ggctatataa aaataattaa aggtaaacat ccacttctaa caggagatgc
     8161 agtaccttta gattttgaaa taggcaaaaa ttatagaagt cttataataa caggtcctaa
     8221 tgcaggagga aaaactgtaa cccttaagac tgttggttta ctaactttaa tggttcaatg
     8281 tggatttgat atttctgcaa aagaaggtag tgaatttagt gtatttgaaa aagtttttgt
     8341 agatataggg gataatcaaa gtattgaaaa tgccttaagt actttttcat cacatataaa
     8401 aaatattgca gagattatga gtttatcaaa taattctact cttgttctgt ttgatgaaat
     8461 aggaagtgga actgaaccta atgaaggagc tggtttagca atatcattat tagaagagtt
     8521 ttacaaaatg ggatgtataa ctatagcatc aactcattat ggagagataa aaaagtttgc
     8581 tacattgcat ccagaatttg aaaatgctgg tatgatgttt gataaagaaa cgttagaacc
     8641 attgtataag ttaactattg gaaaatctga agatagtaat gctcttttta tttctaaaaa
     8701 aatgggaata aaaaatagag ttttagatag agctaaagaa tatgtaattg atagaaacta
     8761 caatttagat ttagttaaaa gaagtaagct acaatctaat attgaagata aaaatagttc
     8821 tgaagtcatt ttaaactata cagattataa tgtaggggat aaggtaaaat tactagatga
     8881 agatgatttt ggaattgtat ataagcctat ggacaagttt aataatgtag aagtattgtt
     8941 taatgataat tttatttcag taaatataaa aagattacaa cttagcataa aagcatcaga
     9001 attgtatcca gaagggtatg atttagatgt attatttact agttttaaag aaagaaaatt
     9061 agaaaaagat attcaaagag gttcaaaaaa agctcttaaa aagattcaga aagaaattag
     9121 agagaataga aaataaatat aaaaagcaca aaagaaagca atctaaagag aaaagtttat
     9181 ctttagattg ctttcttatt aacatatctt cataaatcca atgactaaca agattaaacc
     9241 acttaaccaa gataagttta aatttacgtt tttaacaaac atatttccta gtaaacaacc
     9301 aagtaatact gcaattatat ttgatattaa agaaaataga ataacttgta tataattcac
     9361 acctacaagg ccactaccaa aaccgatagc taatccatct aaacttaaag caagtgcaag
     9421 tgtaaatgct tcagcagatt taagcacttt agatttactt gtatctaaga catcaggttc
     9481 aatacttaca ttaaaattta atttaaggtc taacatatta aaattataat tctttgagtg
     9541 tatagatttt ttcttaaaat aagatttaaa aaaactttca attagtctag cacttcctaa
     9601 taaaaataaa ataaagaaac atattaacat tgttatattt tgaggtaaaa agtctctaac
     9661 tattgtacct gcaaaaagcg atattgctaa aatagctgaa caaactacgc tgataattat
     9721 atttgaatat aagggaactt taactttatt tacgccatat ccaaagcttg tcacaaaggc
     9781 atctatacaa agagaagtaa ctaataaaat tgattcaatc acagtagatt aacatccttt
     9841 ctcttaaatt ttgtacttat attagttata gtattgtaaa aatggataaa ttgttacaga
     9901 taatccaata aattgttgat aaaattccaa catcatacaa cacctaaata acaaaagaat
     9961 catatcatat aataatataa aaccaatcat aataaagcag gtgaaaataa tggaacaaca
    10021 tagtaaggtt gggaatttcg gtgatttgtt gaaagtttta ttagatgaaa aagaattatc
    10081 aatgggagaa ctaagtaaaa aaagtggaat tgataaatct actatatctc gtatagctaa
    10141 taataaacaa aagccaaata taaatcattt agaaaagatg gcaatacatt taaatattaa
    10201 cctagaagaa ctgttgaaag cttcaggata tgaatttaga aacagcaata atcaaatatt
    10261 taatgcagat agtgatttta gtaactttga tgatattctt ggatttgcaa atttaatcaa
    10321 tgataagaac tttaatggaa atattgaaaa agagttaagt aagtgtaagt tatatgtcca
    10381 aacagatgaa ggtaaaaaat tactttttga taattttaat aaaaaaatag attctattga
    10441 aaaacaaggt caatttacag atagattaag acaaatgtat gctgattttt gtacagatgg
    10501 acttacaata aagaaatatt tattgattgg aagtatgcta ctttactttg ttatatcaac
    10561 agatgtgatt ccagacttcg tgttcccaat aggatttatg gatgatttag tagcattaaa
    10621 tatagttaca aaattactta aaaatgataa gtaagtttta gaatcatatt taatatataa
    10681 ataaatcaaa gtatataaaa tttattattt tttatatgaa acaaattaaa agcacaatga
    10741 agtagattaa aaatttaaaa ctaagaatct aatatatatt ttgtaactat ttaagtatat
    10801 aaaataaaat aatatccaaa ctaatacaaa atagtaataa ttgttttgta ctggaggata
    10861 tatatttatg aattgtaaat ggatatctaa aatagatgag agaaaaaaat gtcatagaga
    10921 agcagattct tctggatatt gtatctttca taaagaaaat aagtctgatg aagagataca
    10981 attgatgatg gatactcttc ataaagaaga gataagtgaa tttaatggat ttgtttttga
    11041 aaatgaattt aatgcggaag aaatattaac ttacaattat aaaatactgg atttttctga
    11101 atccatattt aaacaaaaag caaattttaa aaaatatatt tttaaaaaga atattatttt
    11161 taactataca gaatttagag ataaagtttt atttaatggt tgtgtattct tagaaaactg
    11221 tgattttaat agaaccatat ttagtaaaca ctatataaat gaccgtatat ttgaaaaagt
    11281 gaaatttaaa ggacctgatt tggtagttaa taaagtagaa aattttccac gaatggatgg
    11341 aataattttt agcatgtgta caaaatttgt gttaaagaat gttgaatatg gaaaaagtga
    11401 gtatgagcat ggcaagataa attatagaat agctagaaat caagcaacaa aaattggaga
    11461 atatgagatg ataggatttt actattataa ggaaagaatt tatagtagta aaataatgaa
    11521 acgttctaat tatcctacct tcagtgatta tttagttgaa aaattttttg accaaatagc
    11581 acgttacaca acaggttatg gagaaaagcc atggaatata ttattagtta ttatagcaat
    11641 aataagtgtt tttgctttat tatacttatt tgtaggcata gaaagttcaa atagcacact
    11701 tgtagcttta gatataaata atataggtga ttactcacta agtgagattt tcaaaatgta
    11761 tatggattta tggtatttta gcatggctac atttagtaca gtaggatatg gagatatggt
    11821 tgctacaagt ttgattggga aagcattggc aggtatagaa gtattttttg gagttacaat
    11881 aggagcaatt tgggcatctg tgattattaa aagaatgata aggtaatatt tgtgtaaata
    11941 tggataggta ttaattacta gatataaatt ggactaaata gggaaaattt aaatataaat
    12001 atacttaata atccaagaag gtgaaaaatt tgaaaaatgc actaaaaaaa attatatcat
    12061 tagccatgat tctagttttt ataataccaa attattcata tgcagatgat aaatctgtag
    12121 acaagtattc aaaaacatct atacttatag accaagaaac aggtagagtt ttatatagta
    12181 aagaacctga tatgaaggta ccattagcta gtttaagtaa gatgatgact tttttacttg
    12241 caatagaagc tattgaaaat aaagaagtaa aagaaactga taaggtaaaa atagacaaat
    12301 ctattgcatc agtaaaaggg tcaagttata aacttaaaga tggtgaagaa gtgccactta
    12361 tagagcttat gagaggtctt atgattgtat caggaaatga tgcggcaatt gctatagcaa
    12421 aacatgtttg taaaacgaaa gaagcatttg tagatagaat gaataaaaaa gcaaaagaaa
    12481 taggaatgag taatacacat tttctaaatc caaatggtct tccaatatac gatttaagca
    12541 accccaaaaa accagcaaaa gaaaatatct ctactgcaaa agatatagct attcttggaa
    12601 aatacatgtt tgaccattat gaaaaacaag taacagctat aactgatatg gagacatata
    12661 caaattcaga tagaagtttt gaaaaaagca atacaaatgc attattaaga attataccag
    12721 aagtagatgg tataaaaact ggatatactg gcaatgctgg atattgctta tcattttcta
    12781 tggttgtaaa taaaaatgat aaaaatgaga aaaaacgtag agtaataggg gttgtattag
    12841 gtactaacca taaaaataaa agaacctctg cttcattagc tctactgaaa tatggaaaag
    12901 aaaatttcaa tatgaaaaaa gtaattgata aagatagctt tattggtaag aaatatataa
    12961 aaggaacgaa agagttagag gttacattaa aagcaaaaga tgaactttat acaatattaa
    13021 aagatgatga aagtataaag tcagaagtta agattaaaaa tatagagtac cctgttaaaa
    13081 aaggtgatac tatgggtatt ataaaatatt atactaattc aggtgactta cttggaagtg
    13141 tagatatagt aagtaataat agtatagata acgtatcatt aaaaacaaaa ttaaaaatga
    13201 aatttgcaaa ttaaattcaa aaaatggtat aaaatacaca aaaaaatata aaaatagaaa
    13261 atatggaaaa aatattgtta tttcttcacc tctgtgatat actactagag gtgaaaaaat
    13321 aaaataaatc acacacagca ttgggatcct taaaaggtgc ctattacatg gttttttagg
    13381 gagaagatca agctggaggt ataaaaccag gaggtaagat atgtcagtaa tatcaatgaa
    13441 acaattatta gaagcaggtg ttcactttgg tcaccaaaca agaagatgga accctaaaat
    13501 ggctaagtat atattcacag agagaaatgg aatatatata atcgacttac aaaaaacagt
    13561 taaaaaagtt gaagaagctt acaagttcac taaagaagta gctgaaacag gaaagccaat
    13621 cttattcgta ggaactaaaa aacaagctca agatgcgata aaagatgaag ctgaaagatg
    13681 tggaatgtac tttgttaatg aaagatggtt aggtggaatg ttaacaaacc acaaaactat
    13741 aaaaacaaga ataaataaat taagagaatt agaaaaaatg gaagaagaag gcgtatttaa
    13801 tgttcttcct aaaaaagaag ttataaaatt aagagctgaa aaagaaaaac tagaaaaata
    13861 cttaggtgga ataaaagaca tgcctgaatt accaggtgca atgttcgtag ttgacccaag
    13921 aaaagaaaac atagctattc aagaagctca tagattaggt ataccagtag ttggtatagt
    13981 tgatactaac tgtgacccag aacaattaga ctttgctata ccaggaaatg atgacgctat
    14041 aagagccgta aaattaataa ctggtgctat ggcaacagct gtaattgaag gtagacaagg
    14101 tgctgaggaa gaagtagctg aagaccaaga ataataattt gacaattaaa tggtaagggt
    14161 cgcccttacc atttataata tggaatagaa taagtaggag gaaacttaaa aaatggctaa
    14221 tataactgct caaatggtaa aagagttaag agaaagtaca ggcgcaggaa tgatggactg
    14281 caagaaagct ttacaagaag ctgaaggaaa catggaaaaa gcagtagatt tattaagaga
    14341 aaaaggatta tcaaaagctg ctaaaaaagc tggtagagtt gctgctgaag gtctagttgc
    14401 aatagaaatg aatgatgata atacagttgc ttctatggta gaagttaact cagagacaga
    14461 tttcgttgct aaaaacgaag acttcaaagt gttcgttaaa gatgctgctt gtatggcatt
    14521 agctactgat aaagaagata tagcttcttt attaggtgaa actcataaag aaggaataac
    14581 tttacaagaa gttttaaata atagagttgc aaaaataggt gagaaattag actttagaag
    14641 atttgctaaa gttgtaacta atggacaagt tgctggatac attcacggtg gtggaaaaat
    14701 aggtgttctt gttgaaatgg aaacagaagc tagagatgct aaagttttag aattaggtaa
    14761 agatgtagct atgcaagttg ctgctatgaa tcctaagtat gtttcaagag atgaagtaga
    14821 tgctgaatac atagcacatg aaacagaagt attaactcaa caagctttaa atgaaggaaa
    14881 accagctaat atagttgaaa aaatggttaa aggaagatta gaaaaagaat taaaagaggt
    14941 ttgtttatta gagcaaactt tcgttaaaaa tccagatata actgttaaac aattagttgc
    15001 tgatgttgct aaggcagtag gttctgatat aaaagttgtt aaagttgtga gattcgaagt
    15061 tggtgaaggt atacaaaaga gagaagaaaa ctttgcagaa gaagttgcta agcaacttaa
    15121 gtaataacca aagagagaac acgcaggtgt tctctttttt aaaaaataga aaaaattgga
    15181 tatgaaggat tgaaatagga ggtttctgat ggataaacca atgtacaaaa gggtattgtt
    15241 aaaattaagt ggagaagcat tagcaggaga aagaggtttt ggtataaata atgatgtagt
    15301 taatgatatt gctattgcta taaaaaaaat acaagaaata ggtgttgaag tagctgtagt
    15361 agtaggtgga ggaaacttct ggagaggcag aaccagtgaa ggaatggaca gaactactgc
    15421 tgactatata ggaatgttag ctacagttat gaatgcaatg gcgctacagg atgcactaga
    15481 aaatatagat gttgctacaa gagttcaaac tgctatagac atgagacaaa ttgcagaacc
    15541 atatataaga agaagagcag ttagacactt agaaaaggaa agagttgtta tatttggtgc
    15601 aggaacagga aacccttact ttacaacaga tacaactgct gctttacgtg ctgctgagat
    15661 ggaagcagaa gttatattgt tagctaaaaa tgttgatgca gtatatgaca aggatccaaa
    15721 agtacatgca gatgctaaga aatttacaga attaagttac atggaagtta tacagaaaga
    15781 attaaaagta atggattcta cagctacatc tttatgtatg gataataaaa ttccaataaa
    15841 ggtatttgag cttacaacag agaatataat aagagcagta aaaggcgaaa atataggaac
    15901 tactgtaaaa taatgaagaa tgaaggagga gtaaaattat gaaactggaa atacataagc
    15961 aattagaaga gaaaatgaat gggacaattg atgctttaaa gtttgaattt ggaactataa
    16021 gagcaggaag agctaatgca caaatgctag acaaaataag agttgattat tatggaactc
    16081 caactccaat aaatcaaata ggtgctatat ctgtaccaga accaagaata ttaatgatat
    16141 ctccttggga taaatctgct atgcatgaaa tagaaaaagc gatagctaac tcagacttag
    16201 gattaaatcc atcaaatgat ggtgaggtta taagactttc tgtacctgct ttaacagaag
    16261 aaagaagaaa agaattagct aaaaaagcta gtaaagctgc agaagaattt aaagttagaa
    16321 taagaaatga aagaagagac gccaatgaga aaattaaaaa gatggaaaaa ggcggagaat
    16381 taactgaaga tgaattaaaa aaagcacaag atgaagttca aaaaatgact gataaattta
    16441 taaaagaaat agatacatta ttatctaaaa aagaaaagga cataatggag gtataatatt
    16501 gaaaaaatat tataaccttt taggtcttca tgtagatgag gtaaaacaat attttgatga
    16561 gcaaaatata aattataccg taaaatctat tgaaggtaaa aaggataaag aaaaacttgt
    16621 agttcctaga gttataaaaa tatctgagat aggtgatagt gtagaactga taatcacata
    16681 cttttctgac tccttagttt aaggagtcag aacaatattg gaggcaaaat atgaataaca
    16741 acattatata tgatatagac ttaaataaca tacctactca tattgctatt ataatggatg
    16801 gaaatggaag atgggcaaaa gctagatttc ttccaagaac tgcaggacat aaggctggag
    16861 tagaaactat aagagatata gtaaaagaat gctcaaaatt aggtgttaag cacttaacat
    16921 tatatgcatt ttcaacagaa aactggaaaa gaccgaaact tgaagttgac acacttatga
    16981 acttattatc tacatactta agaaatgaaa tagctgaact tcaccaaaac aatgtaaaag
    17041 taactgctat aggagatata agtgctttac caaaaacttg tataagagaa ttaaactcag
    17101 ctaaggaact gacaaaaaac aatacaggag ttaacttaaa tttagcatta aactacggaa
    17161 gtagagcaga tataaaaaat gctcttatag atatagtaaa aaattgtgaa agtggtaaaa
    17221 tagatataaa caatattgat gaagatatta taaaaaacta tttaagcaca aagtctatac
    17281 cagatccaga cttggtaata agaacaagtg gtgaacaaag attgagtaac tttttacttt
    17341 gggaagttgc ttattctgag ttttacttta ctgatataca ttggcctgat tttaaaaaag
    17401 aagaactaca gaaggctata tacgtatatc aaagaaggga tagaagattt ggaggactta
    17461 agtaaggaga ttattttatg cttacaagaa ttattgcttc actagctctg gttccattat
    17521 ttctatttgt tgtatatgga ggtattcctc tatatatagc tgagacagca atagtttata
    17581 ttgcattgca tgagttttat aaggcattta aaataaaaga tgtacatcca atattcatta
    17641 taggatattt attttctatt tatttagctg taaaaaatat ttttaattta ccattagaat
    17701 atacttatgc agtaattttt attttatttt tagcaagtat aatttatatg ttgatgggca
    17761 aaaacaatgt tatagatgtt tcgattacgt ttttaggagt tttttatata ggagtatttt
    17821 tagattttat aattataact ataaatggat ttgaaaaagg tagtatatat gtatggctta
    17881 tatttgtgat atcttttatg acagatatat ttgcatattt tagtggttat ttacttggaa
    17941 aacataagct aattcctaaa gttagtccta aaaagactat agaaggagct attggaggaa
    18001 taataggaag tacactttgt tgtattttat ttggatattt atttggcatt gatttgcttc
    18061 aattagcgat aataggaagt ataggaagtg taatagctca attaggagat ttatttgcat
    18121 cttctataaa gagatatgta gggataaagg attatggaaa gataatacct ggtcatgggg
    18181 gtatattaga tagatttgat agtgttatat tagttgcacc ttttgtatat agtgcgatta
    18241 aattttttat tagataaaga ttggttggat ttaaaagagc aatctataat tgctagtaat
    18301 gaaaatagct atgtaacttt taaatgcaga gatattctgc agttattagt tacatggcta
    18361 ttttcattgt tcaaaaattt agaagacctt ggggaattgt ataagaatag gtattacttt
    18421 ataatagtat actatatttg tagtgagtat attttttata ctataactat cattaaaaaa
    18481 tatttttttg tttaataatt tcgatatttt tagctatatt at
//
LOCUS       DBGJFU010000080        18018 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.080, whole genome shotgun sequence.
ACCESSION   DBGJFU010000080 DBGJFU010000000
VERSION     DBGJFU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.080"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            401..1600
                     /gene="hadA"
                     /locus_tag="ACOIAS_002502"
     CDS             401..1600
                     /gene="hadA"
                     /locus_tag="ACOIAS_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HGZ2702206.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1639..2439
                     /gene="hadI"
                     /locus_tag="ACOIAS_002503"
     CDS             1639..2439
                     /gene="hadI"
                     /locus_tag="ACOIAS_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HGZ2702207.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2454..3680
                     /gene="hadB"
                     /locus_tag="ACOIAS_002504"
     CDS             2454..3680
                     /gene="hadB"
                     /locus_tag="ACOIAS_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HGZ2702208.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3680..4807
                     /gene="hadC"
                     /locus_tag="ACOIAS_002505"
     CDS             3680..4807
                     /gene="hadC"
                     /locus_tag="ACOIAS_002505"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HGZ2702209.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4855..5988
                     /gene="acdB"
                     /locus_tag="ACOIAS_002506"
     CDS             4855..5988
                     /gene="acdB"
                     /locus_tag="ACOIAS_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative isocaproyl-CoA dehydrogenase AcdB"
                     /protein_id="HGZ2702210.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            6021..6806
                     /gene="etfB"
                     /locus_tag="ACOIAS_002507"
     CDS             6021..6806
                     /gene="etfB"
                     /locus_tag="ACOIAS_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HGZ2702211.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6825..7862
                     /locus_tag="ACOIAS_002508"
     CDS             6825..7862
                     /locus_tag="ACOIAS_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGZ2702212.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8345..9976
                     /locus_tag="ACOIAS_002509"
     CDS             8345..9976
                     /locus_tag="ACOIAS_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2702213.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10111..11037)
                     /gene="fba"
                     /locus_tag="ACOIAS_002510"
     CDS             complement(10111..11037)
                     /gene="fba"
                     /locus_tag="ACOIAS_002510"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HGZ2702214.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11367..11696
                     /locus_tag="ACOIAS_002511"
     CDS             11367..11696
                     /locus_tag="ACOIAS_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HGZ2702215.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            11696..12418
                     /locus_tag="ACOIAS_002512"
     CDS             11696..12418
                     /locus_tag="ACOIAS_002512"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGZ2702216.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            12445..13806
                     /gene="rlmD"
                     /locus_tag="ACOIAS_002513"
     CDS             12445..13806
                     /gene="rlmD"
                     /locus_tag="ACOIAS_002513"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGZ2702217.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIIVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            14328..15362
                     /locus_tag="ACOIAS_002514"
     CDS             14328..15362
                     /locus_tag="ACOIAS_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HGZ2702218.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTATSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKEAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSKFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15449..15652
                     /locus_tag="ACOIAS_002515"
     CDS             15449..15652
                     /locus_tag="ACOIAS_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGZ2702219.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15823..16983
                     /locus_tag="ACOIAS_002516"
     CDS             15823..16983
                     /locus_tag="ACOIAS_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HGZ2702220.1"
                     /translation="MKLNKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGSVSAVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSISDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            17241..17381
                     /locus_tag="ACOIAS_002517"
     CDS             17241..17381
                     /locus_tag="ACOIAS_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702221.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7194 a         2039 c         3373 g         5412 t
ORIGIN      
        1 tgataacatg tgtttttgag tttagcaatg aaaacttttt ttgaaatgtc aagctttttc
       61 ataaaaaaat atataaaaaa aacttttata agcatagaaa gaatagcaaa atgcaatgga
      121 tagtggcaag atgccacgat atgaaaaaga ttattcaatt gcatattgtt aaaaaacatt
      181 caaatcaagc gatattttga ttaaatataa agttggcacg ataaatgctt aacaatatag
      241 gtgtaagaca ataaaacaat aaaaaataat tttaataatt tttaagttat tttagataag
      301 attaggaact ttgacatcag acagttaata tcagaaaaaa gtattttatg agtgatttaa
      361 tcgaaatttt ataattgata ctattaagga ggaaaacgtt atgcttttag aaggagttaa
      421 agtagtagaa ctttcaagtt tcatcgcagc accatgttgt gcaaaaatgt taggtgactg
      481 gggtgcagag gttattaaga ttgaacctat agaaggtgat ggaataagag ttatgggtgg
      541 aacatttaaa tctccagcat cagatgatga aaaccctatg tttgaattag aaaatggaaa
      601 taaaaagggt gtaagtatta atgtaaaatc aaaagaagga gtagaaatat tacataaatt
      661 attatcagaa gcagacatat ttgtaactaa tgttagagtt caagcattag aaaaaatggg
      721 tatagcttat gaccaaataa aagataagta tccaggatta atattctctc aaatattagg
      781 atatggtgaa aaaggacctt taaaagataa accaggattt gactatactg catacttcgc
      841 aagaggagga gttagccaat ctgttatgga aaaaggaaca tctccagcaa atacagcagc
      901 aggatttggt gaccactatg caggtctagc actagcagca ggaagtttag cagcattaca
      961 taaaaaagct caaactggta aaggtgagag agtaacagta agtcttttcc atacagctat
     1021 atatggaatg ggaacaatga taacaacagc acaatacgga aatgaaatgc ctttatcaag
     1081 agaaaatcca aacagcccat taatgactac atataaatgt aaagatggaa gatggattca
     1141 attagcttta atacaataca acaagtggtt aggcaaattc tgtaaggtta taaatagaga
     1201 atatatatta gaagacgata gatataataa catagattca atggttaatc atgttgaaga
     1261 tttagttaag atagttggag aagctatgtt agaaaaaaca ttagacgagt ggtcagcttt
     1321 attagaagaa gcagacttac catttgaaaa aattcaaagc tgtgaagatt tattagatga
     1381 cgaacaagct tgggcaaatg acttcttatt taagaaaaca tacgatagcg gaaatacagg
     1441 tgtcttagtt aatactccag ttatgtttag aaatgaagga attaaagaat atacaccagc
     1501 accaaaagta ggtcaacata ctgtagaagt attaaaatct ttaggctacg atgaagagaa
     1561 aataaataac tttaaagata gtaaagttgt aagatattaa ttagtaattc tagatttttt
     1621 aaaaggagaa tgctaaagat gtacacaatg ggattagata taggttcaac tgcatcaaag
     1681 ggagtaatct taaagaatgg ggaagatatt gtagcttctg aaacaatatc ctctggtact
     1741 gggactactg gaccatcaag agttttagaa aaattatatg gcaagacagg tcttgcaaga
     1801 gaagatatta aaaaagttgt agttacagga tatggaagaa tgaactattc agatgctgat
     1861 aagcaaataa gtgaattaag ctgtcatgct agaggggtaa atttcataat tccagagaca
     1921 agaaccatta ttgacatagg tggtcaagat gcaaaggtat taaaattaga taataatgga
     1981 agactattaa actttcttat gaatgacaaa tgtgctgcag gtacaggaag atttttagat
     2041 gtaatggcaa aaataataga ggttgatgta tctgaactcg gaagtatatc tatgaattct
     2101 caaaatgaag tatcaataag cagtacatgt acagtatttg cagagtctga ggttatatca
     2161 catttatctg aaaatgcaaa aattgaagat atagtggcag gtattcatac ttcagtagca
     2221 aagagagttt ctagcctagt aaaaagaata ggagtacaaa gaaatgtagt tatggttggt
     2281 ggagttgcta gaaatagtgg tattgtaaga gctatggcaa gagaaatcaa cacagaaatt
     2341 attgtacctg atatacctca attaactggt gctttaggag cagcgttata tgcttttgat
     2401 gaagcaaaag aatcacaaaa agaagtgaaa aatatataaa aagagggatg aaaatgtctg
     2461 aaaaaaaaga agctagagta gtaattaatg atttattagc tgaacaatat gcaaatgcat
     2521 ttaaagctaa agaagaagga agacctgtag gttggtcaac atcagtattt cctcaagagt
     2581 tagcagaagt atttgactta aacgtattat atccagaaaa ccaagcagct ggagtagcag
     2641 ctaaaaaagg ttctttagaa ttatgtgaaa tagctgaatc taaaggatat tctattgacc
     2701 tatgtgcata tgcaagaaca aattttggtc ttttagaaaa tggtggatgt gaagctttgg
     2761 atatgccggc tccagatttc ctactttgct gtaacaatat atgtaaccaa gttataaaat
     2821 ggtatgaaaa tatttcaaga gaattagata tacctttaat aatgattgat acaactttca
     2881 ataatgaaga cgaagttact caatcaagaa tagattatat taaagctcaa tttgaagaag
     2941 ctataaaaca actagaaatt atatcaggaa agaaatttga ccctaagaag tttgaagaag
     3001 taatgaaaat atcagctgaa aacggaagac tatggaagta ttctatgagt ttaccagcag
     3061 attcttctcc ttctccaatg aatggatttg acttatttac ttacatggct gtaatagttt
     3121 gtgctagagg taaaaaagaa actacagaag catttaagtt acttatagaa gaattagagg
     3181 acaacatgaa aactggtaaa tcttctttca gaggggaaga aaaatacaga ataatgatgg
     3241 aaggtatacc ttgttggcca tatataggat acaagatgaa aacattagct aaatttggag
     3301 ttaacatgac aggtagtgtt tacccacatg cttgggcatt acaatatgaa gttaatgatt
     3361 tagatggaat ggcagtagca tatagtacta tgtttaacaa tgtaaaccta gaccgtatga
     3421 caaaatatag agttgattct ttagtagagg gtaaatgtga tggagcattc tatcatatga
     3481 acagaagctg taaacttatg agtttaatac aatatgaaat gcaaagaaga gcagctgaag
     3541 aaactggatt accatatgct ggatttgatg gtgaccaagc agaccctaga gctttcacta
     3601 atgctcaatt tgaaacaaga attcaaggtt tagttgaagt aatggaagaa agaaaaaaac
     3661 ttaatagagg tgagatataa tggaagctat tttatctaaa atgaaagaag tagttgaaaa
     3721 tccaaatgcg gctgtaaaaa aatataaaag tgaaactggt aaaaaagcta taggttgttt
     3781 cccagtttat tgcccagaag aaattataca tgcagctgga atgcttccag ttggtatatg
     3841 gggaggacaa acagaattag atttagctaa acaatatttc cctgcatttg catgttcaat
     3901 aatgcaatca tgtttagaat atggattaaa aggtgcttat gatgaattat ctggagttat
     3961 tataccaggt atgtgtgata cactaatttg tttaggacaa aactggaaat cagcagtacc
     4021 tcatataaaa tatatatcat tagtacaccc acaaaataga aaacttgaag ctggtgtaaa
     4081 atacttaatc agtgagtaca aaggtgtaaa aagagaactt gaagaaattt gtggatatga
     4141 aatagaagaa gcaaaaattc atgaaagtat agaagtttac aatgaacata gaaaaactat
     4201 gagagacttt gttgaagtag cttataaaca ttctaatact ataaaaccat caataagaag
     4261 cttagtaatt aagagtgggt tctttatgag aaaagaagaa catactgagc tagtgaaaga
     4321 tttaatagca aaattaaatg ctatgccaga agaagtctgt tctggaaaga aagttttatt
     4381 aacaggtata ttagctgatt ctaaagatat attagacatt ttagaagaca acaatatatc
     4441 agttgtagct gacgacttag cacaagaaac aagacaattc agaacagatg taccagcagg
     4501 tgatgatgcg ttagagagat tagcaagaca atggtcaaac atagaaggat gttcattagc
     4561 ttatgaccct aagaaaaaac gtgggtcact tatagtagat gaagttaaaa agaaagatat
     4621 agatggtgtt atcttctgta tgatgaaatt ctgtgaccca gaagaatacg attatccttt
     4681 agttagaaaa gatatagaag atagtggaat acctacttta tatgttgaaa tcgaccaaca
     4741 aactcagaat aatgaacaag ccagaactcg tattcaaact tttgctgaga tgatgagttt
     4801 agcgtaagtt cattaaaata aatttactat aaaatatgaa aattggagga agctatgtta
     4861 tataacaaag aacaagaact tttaagaaaa gcagtaagag attttgttag taaagaatta
     4921 gatactttac cagcagaaat ggataaaaca ggtgtaatgc ctaaggaatt aatcaaaaaa
     4981 ttagctgatg caaaatttat aagcagtaat atcccagaag aatatggtgg tgggggagca
     5041 ggatatgttt cttacgctat agtaatggaa gaaatagcta gaagatgtgc ttcaacagct
     5101 acttttgtta cagctggttc ttctcttgct tcattaccaa tactttacaa tggtactgaa
     5161 gagcaaaaac aaaaatactt aaaaggtata gcaacaggtg aattaatagg agcatttggt
     5221 ttaactgagc caggagctgg ttctgatgca ggtggacaac aaacaacagc agaattagtt
     5281 ggagaccact atatattaaa tggtagaaaa actttcataa ctaatggacc attctgtgat
     5341 gtagctatag taatagcagt tacagataga agtaaaggac ttagaggaac atcagcgttt
     5401 atagtagaaa gtaaatggga tggtttctca acaggagctc atgaagataa gatgggtata
     5461 agaggaactg aaacttctga cttaatattt gaaaacgtta aagttccaaa agaaaactta
     5521 cttggaaaag aaggtcaagg atttaagata gcaatgggta ctctagaagt tggtagaata
     5581 ggtgttgctg ccttagctct aggaatagct caaggtgctt tagatgaagc tgttaaatat
     5641 acaaaacaaa gagttcaatt tggtaagcct atagctaaat tccaaaatac tcaatttact
     5701 atagctgaca tggaaacaaa agtttgtgca gctagaggat tagtttatga tgcagcacaa
     5761 aagagagatg caggaatgag agttgctcaa gaatctgcta tggctaaata ctatgcatca
     5821 gaaattgcaa atgaagttgc ttataaagca ttacaacttc acggtggata tggatttata
     5881 aaagattatg aaatcgaaag aatgtacaga gatgctagaa tcgtatcaat atacgaagga
     5941 acatcagaag ttcaaaaaat ggtaatttca tcaaacgtat taaaataata aataatctaa
     6001 tgaaattgga ggtatagact ttgaaaatat tagtttgcgt aaaacaagtt ccagatacta
     6061 acgaagtaag aataaataaa gaaacaggta ctcttataag agatggagtt ccaagtatat
     6121 taaacccaga tgatgcaaat gcattagagg aagctttaaa aattaaagat gagcatgatg
     6181 atgtaactat aacagttata acaatgggac ctcctcaagc agactttatg ctaagagagt
     6241 gtttagctat gggtgctgat gatgcaatat tattaagtga tagagcattt ggaggagctg
     6301 atacttgggc tacatcaaat actattgcag cgggaatatc taaagtaggt gactatgaca
     6361 taatatttgc aggaagacaa gcaatagatg gagatactgc acaagttgga ccacaaattg
     6421 cagagaaatt agacattcct caagttacat atgtacaaga tttcaaaata gaaggaaaag
     6481 atataatagt tcaaagacaa ttagaagatg gatacgaatt aataaaagta agtacacctg
     6541 tacttttaac agcagtaaaa gagttaaata cacctagata catgtctgtt gataaaatag
     6601 taaaagcata taaacatgat gtaaaagttt ggacaattga tgatttagat gtaaataaag
     6661 aagaagttgg tttaaaagca tcaccaacta aagtatttag atcatttact cctgaaccaa
     6721 gaggaaaagg agaaatctta gaaggtaaag ctgatgaaat agctagtaaa ataataatcg
     6781 gtctaaaaca aaagcacatt atataagttg gaggagtaca aactatgaat gatataaaag
     6841 atttaagttc ttataaaaac gtatggatat ttgcagaaca aagagaagga aaaatagctc
     6901 cagtagttat agaattatta ggagaaggaa gaaaattagc taaagaagta gatgcagaac
     6961 tttgtgcaat attattagga aaagatgttg atggattagc taaagaatta atcacttttg
     7021 gagctgacaa agtttatgtt gcagatgatg ctcttttaga aaaatataca actgatgcat
     7081 atacaaaagt aataaaagat gcaatagatg aaataaaacc agaaataatg cttttcggag
     7141 caactcatat aggtagagac ttagcaccta gaatagcttc aagagttgga actggattaa
     7201 cagctgactg tactaagtta gaaatagacc cagaagataa gaaaataaaa caaactcgtc
     7261 cagcatttgg tggaaacata atggctacaa tcatttgtcc aaaccataga cctcaaatgt
     7321 ctacagttag acctggtgtt atggataaag ctgaaaaaga cgaaacaaga actggtgaag
     7381 ttatagcatt agactacaaa ataactcaag atgatataag aactactgtt ttagaaacag
     7441 ttaaaactaa gaaagattta gtatctctta cagatgcaaa tgttatagta tcaggtggtt
     7501 taggattagg tggaccagaa ggatttgaaa tgcttaagaa attagctgac aaattaggtg
     7561 gagtagttgg ttcttctcgt gcggctgttg atgctggatg gatagaccat tctcaccaag
     7621 taggtcaaac aggaactact gttaaaccaa acctatatat agcttgtggt atatcaggag
     7681 caatacaaca tttagcaggt atgcaatcat cagatttcat aattgctata aacaaaaacc
     7741 cagcagctcc aattttagaa atcgctgact atggagtagt tggagactta catgaaatag
     7801 ttccaatgct tatagaaaaa ttagatagcg ttgatgattt attagaagct ataaaagctt
     7861 aatagataca aactatataa atattactga ttgaataagt cttattagtt agtaacataa
     7921 ttattaaccc tttgataagt tattgctagt gtgtatttgt cagttattaa aaagtctgtt
     7981 tgaataaaat tttataatac aagataatct ggataatcct tatatgtata ttgcttacaa
     8041 attgtgagtt atattctgta aggattatct ttgttttttt aaataaatct taaatttaat
     8101 taaattgtat taaatagaca aataattact aaatatgtga taaaatacaa ttaaaagcaa
     8161 aatgaaattt ttaacaaact ataatcatta aaattatatc ttagttacgt tttaatgaaa
     8221 taaactactt gaaaagacga atattataag gagttgatta tatgtgctga tggtatttat
     8281 tgtaagcaaa caataaattt tgtatgtagt tcaataaatt acttaatgaa aggtgcgaat
     8341 attaatggat aataatttaa tagggcttat agaacatgta gaaaatccag ccattttatg
     8401 taaagaatca ggcgaaataa tatactgtaa tcatctaata gatagtattt ttagttttct
     8461 agatataaaa aagcctagaa atataaatga attagattca aattttgaca aaacagaaat
     8521 actgacagat agtaaaaaga aaatagcttt cagagagtta aggatgactg cacatatata
     8581 caatatgaaa gataataata atgaaaataa tatagtttat ttatttgaga agtctctaat
     8641 atctgataaa gtaattgaag atataataga gcatatagat gaagttgttg ttgtgtttaa
     8701 caaagatggg gtaattgaaa agatgaacac tgtcagtgat gagatattac cttttaaaag
     8761 aactgaggta cttggtagaa atataacaga ccttgtgaga caaggattag ttgaagaacc
     8821 aataatatta aacatgctta aagtgaagaa aaagatttat agaaatatcg tttacccaga
     8881 tgggaagctt attgcatata cggcagttcc aagatgggat tctaagggaa agcttacagg
     8941 aggagtacta acagggagag atatttcaag ggttattaag cttgaatctc agataaaata
     9001 tagtgatata tcagaagaca cagagtatat aagtcaaagt aagattatgg acaatataaa
     9061 aaaagttgtt aagagagctg cagcctcaga ttcttctata tttataaatg gagaatcagg
     9121 tgtaggaaaa gaaataatag caagaacaat atataagtat agttcaagga gagacaaacc
     9181 ctttatagct ataaactgtg gagctatacc aaatgaatta ttagagtcag agttttttgg
     9241 atatgaagaa ggttctttta ctggagctaa gaaaaaaggt aaaaagggac tttttgaaga
     9301 ggcgaatggt ggaactattt tcttggatga gataggtgag ttaccaatgc agatgcaaaa
     9361 aaaattactt agggttatac aagaaaatac tattactaga ataggtggta gtaagcctat
     9421 aaaaatagat gttagatata taagtgctac taatatttct catgaagacc ttagaaataa
     9481 ccttaaattt agacaagatt tgtactatag attaagtgtt attccagtaa aaatacctcc
     9541 tcttagagaa agaaaagagg atatagtacc attagtaaat tatttcttaa aactttacaa
     9601 tgaaaagtac aatagagaag tagaagtttc gcctaaagtt attgagttat tagaagaata
     9661 ttcttggcca ggaaacataa gagaattaaa aaacatcatt gaaagatttg tagtattatc
     9721 agctaaaaat gtaattggag aagatgaatt taacatgctg ataaatttag atatgattga
     9781 taatgaaaca gatgatttat caccaatagt ggtaaatgga attatgaatc ttaatgatgc
     9841 atataaaata gtggaccaaa taatgataag taaggctata aataagtatg gttcaataac
     9901 aaaggctgct gaagttatag gtatatatcc ttctaccatc catagaaaga taaagagtgg
     9961 acatattcac gtttagctgt cagtttaaaa agacaatata attaaaatat tataagatac
    10021 ataatttata caacttaaaa gagatggctt aaaaataaat ttaattactt taagtcatct
    10081 cttttctttt ataaatattt aaaagttata ttagtttata gagttagcag aacctaaaac
    10141 atcatctatc ttgctttcaa ctacagcttg tatagcatct ctaccagcac caatgtattt
    10201 tcttgggtca aattcttttg gattttcagc taagaatttt ctgatagcag cagtcatagc
    10261 tagtcttaag tcagtatcca tgtttatttt acaaacagcc atagaagatg cttttcttaa
    10321 catatctaca ggtacacctt tagcaccagc tatatttcca ccaaattcat tacatgtagc
    10381 aacagcgttt tggtcaacag cagatgcacc atgaagaact attgggaaac ctggtaattt
    10441 gctttggatt tcttctagta tatcgaatct taatttagct tctcctttga atttgaaagc
    10501 accatgagaa gttccaatag ctattgctaa tgaatcaaca cctgttcttt caacgaactc
    10561 aactgcctca gctggttgag tatacttgtg aacatcagaa gtaacatcat cttctgttcc
    10621 agctagaaca ccaagttctg cttcaacaac aacaccttta gagtgtgcat attcaacagc
    10681 ttctttagtt attctaacat tttcttcaaa gtcaaaatga gaaccatcta tcataactga
    10741 agagaaacca gcatcaatac aagttttaat agcatccata tttggaccat ggtctaagtg
    10801 aagagctaca tctacaccta tttcatcaga agcagcctta accatttcaa ctaatgtatg
    10861 aggtccagca tatttaacag ctgacataga agcttgtatc ataacataag aatttttagc
    10921 tttagcagct tttaaaacac cttgtaattg ttctaagtca cttatgttaa atgcacctat
    10981 agcaaatcca ccttcgtaag cttttttaaa catttctttt gtagtaatta atgccatttt
    11041 aaactcctcc tattaacttt taaaatttac ccatcactta tattataacg ttaaatcaaa
    11101 aaaagtatat agtaaattgt aataactata aattacaagt ataatgttaa atagaagaca
    11161 ataggattaa ataaaataat aaagaatata aacaatttag gaaaattagc tatataatgc
    11221 taactataag ggtaggaact accttattag tttggagata ctgataacaa tagttgtctt
    11281 gaccaagaag ttaccacttc aaacgttatc gaagggaatt aagtggatga taattcacta
    11341 aaaatttaaa ataaaggaga taaactatgc cacagataaa aataagagga attaatgaaa
    11401 atgatatatg taaaataagt gaaaaaatga taaatgattt agttgaagct gtaaaatgtc
    11461 caagagacta ctttgaaata gaatgtataa agtcagttgc aataagagat ggtaaaatag
    11521 cagatgtata tccttttgtt gaagtagctt ggtttgatag aggacaagaa gtacaagata
    11581 tagtagcaag aataataact gatagtataa gaaataattt agatgtagaa agtatggatt
    11641 tagcatttac agtatttgaa aaagaaaaat attatgagaa tggagagcat ttttaatggc
    11701 taaaaagcaa cgtatagata aaattctttc aaatttaggt tatggaagca gaagtgaaat
    11761 aaagaagtat tgtaaacaag ggtctgtagt agtaaatgga agtgaagttt caaatccagg
    11821 aactcaagta gacactgaaa atgatgagat attatttaat ggagaagaag ttatatatag
    11881 agaatatata tatttgatga tgaataaacc tgatggatat atatcagcaa ctacagataa
    11941 atatgaccca acagtattag atttaattga tttatcatac ttagcttttg aaccatttcc
    12001 tgttggaagg ttagataaag acacagaggg attattagtt ttaactaatg atggaaagct
    12061 ttctcataga gttttatctc ctaagaagca tgtgcctaag acttattatg caaaaattga
    12121 tggagtagtt actgaagaag atgtagaagc ttttttagaa ggtgtagtgc ttgatgacgg
    12181 atataaaact atgccatcac agttaaatat attaaaaagt gatgatgaat cagaaataga
    12241 gcttacaata catgaaggta agtttcacca agtaaaaaga atgtttgaaa gcgtaggcaa
    12301 aaaagtagta tacttaaaaa gattgtctat gggcaattta aaattggatg aaagtttaga
    12361 gttaggtgag tatagagagt taacagatga agaagtaaaa ttgattgaag aaagataata
    12421 aagatactat agtaaattgg aggaatgtct gtgaagaaaa aagatgttat agaatttgaa
    12481 gtagataaaa tggaatttgg tggtacatct ttaagccaag ttggagatag agttatccat
    12541 atgaagggtg gaataagtgg tcaaaaggtg agagctggag ttaaaaaagt tagaagtaag
    12601 aaagctgaag ttaagatgat agaattatta gagaactcac cacttgaaac tgaagaaact
    12661 tgcaagcact ttagagagtg tggaggatgt acactattgt ctgtgccata tgaaaaacaa
    12721 ttagaaataa aagaaaaaca agttatggat ttatttttaa agcaagattt atttggattt
    12781 cagtttttag ggatagaagg aagtccagaa aacaaatatt atagaaataa gatggaatat
    12841 acttttggag atgaagtaaa aaatggacct ttgacattag gtcttcataa aaaaggaaag
    12901 catatagata tacaaactgt tgaggagtgt atgttggttg atgaagactt ctcaaagata
    12961 ttagtttcta gtgtagagtt tttcaatgaa aaaaagcttc cttattatag aacaatgaat
    13021 cacaaaggat atttgagaca tcttgtagtt agaaaaggga ttcacactaa tgagattata
    13081 gttaatatag taacttcttc tcaagaagat tttgatatgt atgagtttaa agatatgctt
    13141 ttagggattg atttaaaagc tgagttagta ggtgtacttc atactattaa tgatggttta
    13201 gcagatgcag tccaatgtga tgagttaaga gtattgtatg gaagagatta tatacaagaa
    13261 gaaattttag gtcttaagtt taagatttca ccattttcgt ttttccaaac caatactaag
    13321 ggagctgagg ttctttatag tattgctagg gattttattg gagattacaa tgataaagtt
    13381 gtatttgact tgtatagtgg tactgggtct attggacagg ttatggctgg tgctgctaag
    13441 aaagtgtatg gtattgagat agttgaggaa gctgttgttg ctgctaatga gaatgctaag
    13501 ttgaatggac ttactaattg tgagtttatt gctggggatg tggctaaggt tgttaaggat
    13561 ttaaaggaca agcctgattt gattattgtt gaccctccta gaccaggcat tcataaggat
    13621 gctattaggg atatttgtgg atttggtgct aaggagattg tttatatttc ttgtaatcct
    13681 aagtctttgg ttgttgattt ggttgatttt aaggggtatg gttatgagat taagatggtt
    13741 aagtgtatgg atatgttccc aaatacgcca cattgtgaga ctgtagttaa acttattaga
    13801 gaataacagg gtttcagact tttatagtgt gtcttaaaag tagatttgcc acctatttgc
    13861 caccataatt atttttgggt ggcaaattta ttctacaatg atttcaaaaa tatttaccgt
    13921 ctggtttctc atgttgttag ttacatgtaa atatgtatcc atagttgttg aagttttagc
    13981 atgtccgagt atatgctgga tatcttttat attagtacca ttttctaata acatagtatc
    14041 agggttttta aaataaagtg tgtattcttc taaagtagtg ataattaaaa tatcaaatat
    14101 cactatacaa agaaaagtgc acgcttttta tgtttaacaa aaacgaaata ttaaaaaact
    14161 tattgaagaa tatgatgtca aaacaactac agatattcaa gatatgctaa aagatttatt
    14221 tacaaataca attcatcaaa tatttacatt gtcgaatttt aacggtttaa acataatata
    14281 ttgactgata taataaaatc atagaatatt ttagggggaa atagattatg tttaagaaaa
    14341 agttaatagc tgttgcaatg agtataacta tgattagtgt agggagcttt tcgtatgctc
    14401 attcaggaag aactgattca tcaggaggtc atcgtgataa taaaaataaa tctggattag
    14461 gaagttatca ctatcattgt ggtggttatc cagctcattt acataataat ggtggttgtc
    14521 cttatactgg aggaggaagc agttctggaa cagcaacttc ggttaataat gaagaaaagc
    14581 aaaagaggtc tgttggagaa aaaggatata atcaagggta tgaagatggg tataaaggaa
    14641 attatagttc atcaaattat agtggagatt atagtgatac atatgaatca aagtactcag
    14701 aaggatatga aaaaggtaag gctaaattag aagaggaaga aaaggaggct aaagagactg
    14761 gatataatct agggcttaca ggtgctaaat ctaataatac atatgaaaaa gaagttttaa
    14821 aaaatgctta tgataccgga tattcaactg gatacaatga atataagact aaaaagatag
    14881 aagaatataa agctaaaggg atagaggata gcaataaaga taaagaaaaa atgacatttg
    14941 aagaaaacat agattctaaa tttatagatg cttataataa tgcttatgac gaaatacaag
    15001 aacaattaaa aaatgattac actactcaag gatttgagag tgctatcaaa ggagaaagat
    15061 ttgacacatc aactataggt aatgttaaat atgctaattg gtttaaagaa ggatatgata
    15121 atggtaaggt taagttacct aaagcaaaag aaagtgtata taatcaaggt tacaatgaag
    15181 aagattttag tataccagat gaaatgaagt ctattgaaac taagttaaaa ggtgtatatg
    15241 atgaaggttt agaaaaaaga gaagaagaga aaagtagaaa tgctacttat ggggttggag
    15301 ctggaacagc agctgtagtt gctggagtag tatataagaa gaaaaaacgt aaaaagatat
    15361 agatgctaga tataccaatg atggcaagtg tataacaaat ataattttaa gtataaatat
    15421 ttaaaatata gaagggaagg ttatatctat gggatttaac tttaggaaaa gcataaatct
    15481 aggaggagga ctcaaattaa atttaggtaa gaagagtgta ggtataagtg ctggtgttaa
    15541 aggtgctaga gtgtcagtta attcaaaagg aagaaaatca gctacattga gtatacctgg
    15601 aactggaata tcatatacaa aaactagtac aaagaaacgt aaacctaaat aaatatttga
    15661 tatactaaga aatagactgt tacaaatagt ctatttttta gtatatagat ttaaaaagtg
    15721 acaaaatttt gaaatataaa ctaatttcat aaaggaaaat attcttttat gtagaattat
    15781 aatatttaag taatattata aaatttatag gaggatttca ttatgaaatt aaacaaaaca
    15841 ctattggcaa tagggatgtc tgtttcatta ttgattgcta gttgtccaac atcaaacgct
    15901 cttagttcta ttaattcaat aaaaggtaca aataagtatg aaacagcagg tttaatagca
    15961 gataaacaaa attatgatat tgcaatactt attaattctg aaaatggatt atctgatgga
    16021 ttgtcagcaa gtggattgtc aggagctaaa aatgccccaa tattgttaac taagaaagat
    16081 acaatccctt cagaaacatt aaaaagatta gaaaaggtaa aagaagtata tctaatcgga
    16141 ggaaaaaatt cgataggagc atcagttcag aaaatattaa gtgataaaaa aataagcata
    16201 aatagaataa gtggaactga tagaatagat acaagtcata aagttgcaaa agaaatagga
    16261 agtgtaagtg ctgttaaaaa agtaatatta actaatgctt ttaaaggtga accagatgct
    16321 atgagtgttg cttctatagc agttagagat aaatctccaa tagtattaac tgatggaaac
    16381 actacaagtt ttgctacaga tggattagaa acttatgcaa ttggtggaaa agtcagtatt
    16441 agtgataagt taataaatga aactaaagca acaagaatag gtggagtaga tagatacgat
    16501 acaaataaac aaataataaa taaattttat aatggctcaa aagaatttta catagcaagt
    16561 gggactgatt tagtttattc tttagttggt tctacaattg cacaaaaaac acctatagtg
    16621 ttagttggaa atggaagtaa taagagtata ttaaaaaatg caagtaaaat aactgctatt
    16681 ggaaatattg atgatgttgt tataaaacaa tgtttaaata taactaataa tgttggggat
    16741 tcaaatacag gcattgtaaa agatactaat tcaaataata cgaacaaacc aagtaatgag
    16801 cctagctcta ataatcctgg attaactgat aaaagtacag tatatgtaac tcctaatgga
    16861 aaatcatatc atctaacaaa aaattgttct acactaaaaa gaagtaaaac aataaataaa
    16921 gtaacattaa aacaagctaa gtcacaaggt aaaatagacc cttgtaatgt ttgtgtaaaa
    16981 taatgtatta gccaaatata tattaaaaaa gacaattaat taagattgtc ttttttattg
    17041 aagaaatata aatttatatt atgtattatt aaaaaaaatt attaattata aaaaataata
    17101 ataaagacga taagtattaa aattttatgt ttccaatatt tatgatataa ttaatatggc
    17161 aaggaataaa tatttgaaaa agctatgagt ggtgtttcca tggaagtttc ttcactttct
    17221 tatgagagga ggtgaaaact atggaaaatc taatgatgag tattataact ggtgttatag
    17281 ccagttacat ttacgataaa ttaaagagcc actctgagcg accaactaag agtggctggg
    17341 aacttaatat aaagttccag aaaagtaaat cttcaaaata atttgttgaa ggaaacatca
    17401 ctcaaagtta agtaaaatat gtttccttgc ttttattata tcacaaatta aaaatattaa
    17461 cacaaaattt tgtgaatata tacttattat tgttctaaaa aaatatgtaa ataattgatt
    17521 tgatttcata gaattattgt tgtaatgttt agttaaatat acacaatctc aattaaagaa
    17581 gataagtact ataaattcat atcaaaaaaa tacataaact tttaagttat aattttataa
    17641 aaagtctttt agtatcatta tttcaagagg aattaacata tgaaaaaata acttcattag
    17701 aaaagtggat aaaaaactgg taagaagatg actaaagatt acatatacta ttacttaaat
    17761 taaaaattta atataattta taatgtatac aatttattat aaaaaattta attttaattt
    17821 tctatttttg atactataat atataatctt gataatttaa ttaaaaatct agattttaca
    17881 taccaaactg gtttcttatt tcgctatctt tacttatgaa ctcatatatt cctataaagg
    17941 ttattttaga aaaagttggt tatttaaaca ttaataccac aatttctata gattgtttgg
    18001 tgaataatct ataattat
//
LOCUS       DBGJFU010000081        17620 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.081, whole genome shotgun sequence.
ACCESSION   DBGJFU010000081 DBGJFU010000000
VERSION     DBGJFU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.081"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            83..418
                     /locus_tag="ACOIAS_002518"
     CDS             83..418
                     /locus_tag="ACOIAS_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2702222.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            555..1832
                     /locus_tag="ACOIAS_002519"
     CDS             555..1832
                     /locus_tag="ACOIAS_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HGZ2702223.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1953..3341
                     /locus_tag="ACOIAS_002520"
     CDS             1953..3341
                     /locus_tag="ACOIAS_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2702224.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3620..4258
                     /locus_tag="ACOIAS_002521"
     CDS             3620..4258
                     /locus_tag="ACOIAS_002521"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HGZ2702225.1"
                     /translation="MKAKEEIEWLLNRDYKIDPVVTFVGDRYQFSIRQRDALKRAVCT
                     DEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISIEVGLSGGTLVIGSDGNIRDLAGLRG
                     TYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDAPVSNSGNLKYRILEHAKTWGIETEV
                     ELVKNADVVLEKLDRVVSSDAVIVDKCISYFNVARGIIEEYIKECNIINLNK"
     gene            4376..5245
                     /locus_tag="ACOIAS_002522"
     CDS             4376..5245
                     /locus_tag="ACOIAS_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HGZ2702226.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5400..6545)
                     /locus_tag="ACOIAS_002523"
     CDS             complement(5400..6545)
                     /locus_tag="ACOIAS_002523"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGZ2702227.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(6878..7654)
                     /locus_tag="ACOIAS_002524"
     CDS             complement(6878..7654)
                     /locus_tag="ACOIAS_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGZ2702228.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8236..9210
                     /locus_tag="ACOIAS_002525"
     CDS             8236..9210
                     /locus_tag="ACOIAS_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HGZ2702229.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9308..9613
                     /locus_tag="ACOIAS_002526"
     CDS             9308..9613
                     /locus_tag="ACOIAS_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HGZ2702230.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9769..10929
                     /locus_tag="ACOIAS_002527"
     CDS             9769..10929
                     /locus_tag="ACOIAS_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HGZ2702231.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            10979..11881
                     /locus_tag="ACOIAS_002528"
     CDS             10979..11881
                     /locus_tag="ACOIAS_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGZ2702232.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            11961..12854
                     /locus_tag="ACOIAS_002529"
     CDS             11961..12854
                     /locus_tag="ACOIAS_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HGZ2702233.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      12907..13088
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13316..14446
                     /locus_tag="ACOIAS_002530"
     CDS             13316..14446
                     /locus_tag="ACOIAS_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2702234.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14518..15543
                     /locus_tag="ACOIAS_002531"
     CDS             14518..15543
                     /locus_tag="ACOIAS_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGZ2702235.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15546..16742
                     /locus_tag="ACOIAS_002532"
     CDS             15546..16742
                     /locus_tag="ACOIAS_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702236.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFNLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(16870..>17620)
                     /locus_tag="ACOIAS_002533"
     CDS             complement(16870..>17620)
                     /locus_tag="ACOIAS_002533"
                     /inference="COORDINATES: protein motif:HMM:NF040187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGZ2702237.1"
                     /translation="NKFMKVILMKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRL
                     ENMDLRGYCLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGA
                     SFRFCDLSNSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNF
                     RMVQLLIPSDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGET
                     IVAEDFNENRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         6751 a         1970 c         3083 g         5816 t
ORIGIN      
        1 gctaggtata tttataatat taataaatat attgctataa aagtttgttt aataaattaa
       61 ataaagaaaa gaggagaata aaatgtacaa catagatgat atgatatata attgtccagt
      121 agaagcttta tcaaatattt taggtaaaaa atgggtggct ataattatat ggaaaataca
      181 agaagataaa aaacgatttg gagagctaca aagagctata ccagactgta gtaagaaaat
      241 gttagttcaa caactagatt ttttaattga acaggggatt atagtcaacc aaaaaaagtt
      301 ggttaataat gttgtagagt caacatattt tttaagtgaa tcaggattac gattgcttcc
      361 tgtcatggaa aaaatgatta tgtggagcaa taaaaattta aattgtgata aatactaaaa
      421 tttactctgt aagtaatgaa aatttaatta agctatgagt agaaaaatgt attaaaatta
      481 cataattttt tagttagatt ttaatttaga aatgggattg taatattgga aataaaataa
      541 ggaggaattt taatttgcaa aatgtaaatt tatttttagt atttatagaa ggtatagtat
      601 cattcttttc accatgtata ctgccaattt tgcctattta tttgagtata ttgtcaaata
      661 gtagtgtaga aaatttaaaa gaaggaaaga cgagttttat aggaagttct ttatttaaaa
      721 atactatatt ttttgctctt ggaatctcaa caacattctt tatattgggt tcatcagtaa
      781 aagtattgag tatgtttttt aatgaaaata aagatttaat aatgtttatt ggtgggatta
      841 taattattat catgggatta ttttacatgg gaataattaa atcttcaata ttgaatagag
      901 aaaaaagatt taatgttaag tttaaagaaa tgaaagcaat aacagctttt atattaggtt
      961 ttacgtttag ttttggatgg acaccttgta ttggtccaat attggcatct gtattagtca
     1021 tggtttcaag ttcaagtaat catcttagtg ccaatctttt aatagcagta tatactattg
     1081 gttttatttt gccattcata attacagcta tgttttacag taaactattt aaaactatag
     1141 ataagataaa atctaacatg gaaattatta aaaaaatagg tgggattata cttatagtat
     1201 caggaatatt aatgatggta aatggatttg gaagtataag caaacacttt aatacgtcac
     1261 aaaatagtaa aattgaaagc aagcaagaag aaaataagcg agaaaattct actgataaag
     1321 aagaaaattc tgatggtaat gatagtcaaa aagactctaa taatgacaat aatgataaag
     1381 ggtctaatga tgaagataga ataaagtcta ttgattttac tttaacagac cagtatggta
     1441 aaacacataa attaagtgat tatgaaggca aagttgtatt cttaaatttc tgggctacat
     1501 ggtgtcctcc atgtaaagaa gaaatgcctt atatagaaca actgtataaa gactataata
     1561 aaaataatga tgatgtagta attttaggag tagcttcacc taatttaggt agagaaggtt
     1621 caagggaaca tgtagttaac ttcttaaaag aacaaggtta tacattccct gttgtacttg
     1681 atgaagatgg agcattagca tatcaatatg gaattaatgc ttttccaact acatttataa
     1741 ttgacaagga aggatatgta actcagtata ttccaggagc tatggataag gcaactatgg
     1801 catcatttat tgaaaatcag agaaataaat aaaagtattt aagtataagt ttataaatca
     1861 atttaaggtt acaaaaaagt gcctaattgt accacctaaa taatcttgtt atagtaaaag
     1921 tataaacaat acaagaaagg gttgaatttt atatgaaaaa aacatttgat gctataataa
     1981 taggatttgg aaaaggtgga aaaacattag caggggattt ggcaaacagg ggattaaaag
     2041 tagctttaat agaaaagtca aacaagatgt atggtggaac atgtgtaaat gtggcatgta
     2101 taccaacaaa aagcttagaa aattcagcaa acagcgtaaa aacaaagaat ataaattctt
     2161 gggatgaagt acaagctgaa tatgagaaag ctatagataa aaaagaaact ttaattacaa
     2221 aactcagaga agctaactat aataaattaa attcaaatga gaatgtaact atattcacag
     2281 gaatgggaac tttcattgat gaaaaaacag ttcaagtcaa aactgaaaat gaaatttatg
     2341 agttagtagc agataatata tttattaata ctggttcaag accatttata cctaatatta
     2401 agggaattga aaataagaat atagtatatg atagtgagtc tcttatgaat ctaagagttt
     2461 tacctaaaaa aatgacaatt ataggagctg gatttatagg tttagaattt gctggtattt
     2521 atagttcatt tggagcagaa gttacaatat taaactctaa taatggcatt ttaccaaatg
     2581 aagatgttga ggattcagaa gaaataataa aattacttgc aaagagaaat gtaaaaattg
     2641 ttaataatgc aaatataaaa gagattaaag aagtttctga attagcaata gtagaatatg
     2701 aagtagatgg taaatctaaa gaattaacaa gtaatatgat acttgtagct acaggaagaa
     2761 aagcaaacac tgaagggcta gggctagaaa atgcaggtat agaattaaat gaaagaggat
     2821 ttattaaagt ctcagaaact cttaagacta ataaagagca tatatgggct ataggagata
     2881 taaatggagg tccacaattt acttatattt cattggatga ttatcgcata gttattaatc
     2941 agttatttgg agataagact agaacaacta atgatagaaa gaatatccca aattctatat
     3001 ttataagtcc agcattttca agagttggtc ttaatgttaa gcaagctaaa gaaaagggat
     3061 atgaagtatt agtggcaaag atgccagtag aagcaattcc aagagctaaa cagattggca
     3121 aggcagatgg gtttataaaa atagtgatag ataaaaaatc caataagata cttggagcta
     3181 gtatgatatg tgaaaattca agtgagatta tccatcttat tcaattagct gtagatttag
     3241 aagtagagta tacatattta agagaccgtg tttatgcaca tccaactatg acagaagctc
     3301 ttaatgatat tctatctcct aatatgataa aagaagtata acataagttt aatatataaa
     3361 tctaaaatta tcattataag tagattttag aaagaggcta tctcaaaaaa tagagataag
     3421 tctctttttt actgtttaaa taataaaaat atattttaat ggtattaaaa attatcttaa
     3481 aaggtgaaat aatctgtatt taatgtagta cagttatatc tttaaagcaa ggtggtgaaa
     3541 tgatataaat aagatatcta aaagagggtt tgatgatagt gataaaagat ggttttcaag
     3601 taaagaatta acaagactta taaaggctaa agaagaaata gaatggttat taaataggga
     3661 ttacaaaata gacccagtcg ttacttttgt tggtgataga tatcagtttt ctataagaca
     3721 aagagatgca ttaaaaaggg cagtctgtac tgatgaaaaa aatattataa gacaatcaaa
     3781 gaggctttct ctagataaaa tcaaagaaga taccgtaaat atagatggct ttaatttgat
     3841 aatatccata gaggtaggat tatctggtgg tactctagta ataggaagtg atggcaatat
     3901 aagagattta gcaggtctta ggggaaccta taagattata gataaaacag aagaagcttt
     3961 gaatctgata ggtaaatttt ttaataaata caatgcacag aatcttaaat tttatttaga
     4021 tgctccagtt tctaattcag gaaatttaaa gtataggata ttagagcatg ccaaaacttg
     4081 gggaattgaa actgaagtag aattagtaaa aaatgcagat gttgtattgg aaaaattaga
     4141 tagagtagtt agtagtgatg cagtcatagt tgacaagtgt ataagttatt ttaatgtggc
     4201 tagaggtata attgaagagt atattaaaga atgtaatatc ataaatttaa ataaatagat
     4261 aaatataggt gagaatatat aagtatatgt agcaatattc ttagtaatac aagtaaagtt
     4321 tgaaaatagg agaacgaata catatcgaat ttcataaaat gttgtaaaaa gacatatgag
     4381 aggatggata tgtgtgaaat taactaaact aaatattaaa aaatatagag cacctatata
     4441 taaatatgct ttagcaaata taaatttgag gtctgctaaa tctaccaatt caagtatcat
     4501 aacagtaata cctcagggag ccaaaatgga agtattggat gaggaagatg actggattaa
     4561 ggtgatgtac aattctcaag aaggatatgt gtataaggag ttggtatcag taagcgaata
     4621 tgcttggagt aacttaaact taagagaaga taaatctaca acttccaata taattacagt
     4681 aattcctgaa aaatctaggg ttgaagtcct tcaagtagat ggagattgga gtaaggttgt
     4741 atatgatgat aaaataggat atgtttttaa ttatttttta tcaattgatg ggaataaacc
     4801 aaatgaatta gattataagt atttttatac tgacatgatt aaatttgtta acgaaaataa
     4861 tattaaaagt actagcgatt atctaattgt aacagacctt agaaataaat atacttatat
     4921 ttttaagaaa gataatggag gatggggaca actttacaag tggcaatgta ctataggcaa
     4981 gcctgagact ccaactataa caggtatatt ttatataagt ggaagaaaac cttcttttgg
     5041 aacggatgag tattcagtta agtatgctac tagaattaaa ggtggatatt actatcattc
     5101 tgtactatat gattcaacag gaagttatat aatagatggc agacttggtg aagctcttag
     5161 tcatggatgt ataagattaa gtacggaaaa tgcaaagtgg atatatgata atataccaga
     5221 tacaacaaca gttataattc attaagaaaa attttgagtt ttttattggg ataatgattc
     5281 ttaaattaat aggattatat attaacttct cataaagagt actatttaaa aagtgtaata
     5341 agagagtgct attaacaaaa caaagatatt ttgaggcact ctcttataaa tatacataat
     5401 taagatattt tcataaacaa ttctataatc ttatctttat caaagaaaac aggattagca
     5461 ccagcacaag catctttcat agagttttct gcaagcaatt caaaattagg attatcaaca
     5521 cctacatctt ttaaagattt tgggattcca acctcttcag aaagagcttt tatcttatct
     5581 attacaaagt caacacactc tttatctgat ttattattta catccattcc aataacttca
     5641 gctattggtc ggaatttggc aggggctgat ttagcatttt cctcctcaac tattggaagt
     5701 agcatggcat tacatactcc atgaggtaaa tcatataaac ctcctaattg atgagccata
     5761 gcatgtacat ttccaagacc tgcattacta aaggcaattc catttaaaaa acaagcataa
     5821 cacatttgtt ctctagcttc tatgtcatta ccatttttaa cagctcttgg aaggtattta
     5881 aatatctgct ttatagcata taaggcagtt gaatctgtaa catcataagc accattagct
     5941 actacagctt ctatagcatg agttagtgca tccataccag ttgctgctgt taaattagat
     6001 ggtttgctaa tcattaattc tgggtcattt actgtcattg tggctagtga attggtatca
     6061 accattatca tctttacatg tctgtcttca tcagttataa catagtttat tgtaacttca
     6121 gcagatgtac cagcagtagt agttatagct atgataggaa gacatttttt agaagttttg
     6181 ttgatacctt catagtctct aatatctcct ccattagtgg ctaatataga gatagcttta
     6241 ccacagtctt gtggagaacc acctcctata gttataacga agtcgcaatt ttctgatttt
     6301 aatattttaa gtcctttatt cacattagtt acagttggat tttgtttaac ttcatcataa
     6361 acaacaaagt ctatatctat ctgagataaa atatctgtta ctttttttac agtaccattt
     6421 gaaactagaa atttatcact aacaataaat gcttttttag ttcctaatga tttaattgaa
     6481 tctttagctt cattaagaca tcctttccca agtaaattta ttggtggtac ataataaatg
     6541 ctcataacta aaatctcctt ttattttcat tgatttttat gtaaaaatat atatctaaca
     6601 attttcacta atttactcat aatatatcgt atcttagatt gagatagggt tgctggttct
     6661 tatataagaa cttaattcgt aattacgatt atgattagaa tttaacatag tatttttatc
     6721 atgtcaataa aaatataaaa aatattataa aaaaaacaaa gttactgtag ttaaatcagc
     6781 aactttgttt ttatagtata aatcaaataa atacattagt ttaaaacaaa atttttaatt
     6841 attatgtttt gatatcttcc atatacagaa tattaatcta atttattaca atgtcttcat
     6901 tacgttcttc catcattgat tgaatatttt tctgagcatt taagagtaat ttttttatca
     6961 gttctatttt ttcgtcatta gctctaaata aaggcagtgt tacactaaca gcagctctta
     7021 tacatccatt ttttcttatt ggtacggcaa gacattttag gtgttccatt gattcctcac
     7081 actctgaagc tatttgtgtt ctttgaactt caagaagctg gtcgtataaa acatcaaaat
     7141 ctgtaattga tttttctgta aaacctttta gaccatctgg atataagctt attagttttt
     7201 ccttgtcaaa atcacataaa agagcttttc caagacctgt acaattagca ggtatttttt
     7261 ttccaacata agaaataagt ctaatcggtt ctggagaatc tacttttcca atataaagaa
     7321 cttcattatt gtcaagtata ccaagctgac aagtttcaga acaggaatta actatattag
     7381 tcatttcgtc aattatatag ttaaaaatag ttcttttttc aaaaaatgaa gaccctaatg
     7441 tgtaagaacg tatccctata gaatatttgg atgtgttttt atcaatagta atgtattttc
     7501 tagcattaag tgtatgaact ataggaaaga tgctactttt tggagcattg ataacatctg
     7561 ctatttctgt aagagtatat ccattttcgg attctgctaa tgcttctaaa atatctaata
     7621 tcctagctgt tgggcgatga ttatctgaag tcataaattt ccccctcttt ttattaattt
     7681 aaagtattag aatatgttga ttttagcaca ctatacgata ttttacaatg tagaaaataa
     7741 atatgtaaat ttatatataa ataagctctt gatttagata cagatagata tttttttaga
     7801 ctgtacatta aggaataact aatttccaaa atatacctaa agaaagtaag tgatatttag
     7861 ttatataatt taagtaaata tatgcaatag ttatttttat atgtaaatat caaaatatta
     7921 tgaacttata ttttatctat tttttacact gtataaaaac tttatagttt tgaatactat
     7981 ataattttaa atacgctgta tttttgcata taaggaagtc atttgataat atacaaggta
     8041 ttatcagaaa gtttgaaaaa attttgacaa aaatttattt tttttattga ctcataaata
     8101 ggaaaatgct atcattaatt caaagattcg taattaaaaa cttagttctt aaatacgaac
     8161 cgaaacggcc gttagaatta aagctaaaaa cttaataaga gtaattaaat atcatgtttt
     8221 aggaggatta actttatgat aatggatttt attaaaaaaa ttcctgcagg tatgatgata
     8281 gtgccaatgt ttatagcagc tttcattaat accttttgcc cagatattgt acaaataggt
     8341 tcgtttacga cagcagtatt ttcaagtgtt gggtctgcaa ctataattgg ggttcaactg
     8401 gtatgcttgg gaactcagct tcaatttaga gaaatgccaa aagttttaaa gagaggtggt
     8461 atacttcttg gttctaaatt tgctataggt gctttgatag gtatacttat aggaaagcta
     8521 tttggaatgg atggtgtcct tggtctgaca acattggctg ttataagtgc agttacaaat
     8581 agtaatggta gtatatatct ttcattgatg acttcatatg gagatgaaac agattgtgca
     8641 actatgagtt tacttacttt aaatgatgga ccatttttta cattaatagc tcttgggact
     8701 tcaggtcttg caaatgtacc attgttgtca ttattggctg caatagtgcc aatacttgtt
     8761 ggtatgataa taggaaattt tgacaaaaaa atgaaagagt ttttagagcc agggtgtaat
     8821 ttactcgtac cttttgtagg gtttgcactt ggtagtagca taaatcttgc aagtatacta
     8881 aaaggaggtt tatcaggagt attacttgga ttaatatctg tatttgtagg aggtatattt
     8941 atagtattct gtgataaatt tataggaaaa cgtccaggat atgcaggatg ggctgtagct
     9001 acaactgctg gaaatgcaat tgctgtacct gctgctgttg cattagttga cccatcatgg
     9061 caaccatatg ttgcaacagc tacaacacaa gtggcagccg cagtagttgt cactgcaata
     9121 ttagtacctc ttataactag ttggtgggct aagaagtatg gatgtcctaa gttcccaaaa
     9181 caaaatgagg atggtacaat aacagcttaa tttatattac agtgagttgt tggtaaaata
     9241 ataaattctt taattagatt ttaggatatt aataaaatta tattatttat aaaggagagc
     9301 aagaataatg ataaatgcag tgataattga tgaaaaagat aatgttgcag tagctattga
     9361 agcaatagct aaaggtagtg aaattagttt taaattaaaa gacaaaacag taaaaacgat
     9421 tacagcacta gatgatataa ctatttatca taaattggct ataaaagata tggccaaagg
     9481 tgataaagtt actaaatatg gtgaacatat aggagaagca aatcaggaaa taaaaagtgg
     9541 ccaacatgta catattcata atgttagaag tgttagagag gatttggata aacaagttat
     9601 gactaatata taaaataata tttatagagt tataaaagtt gtattcattg cttttagtgt
     9661 caaatagttt ttaaataggt ttttagctgt ttgattagtg agtgtaataa aaattataat
     9721 atataaaaat tatagaaagt atatagacta ttttaggagg aaatcagtat gaaattttat
     9781 ggatataaaa gaccagatgg aagagttggt ataagaaacc acatattaat attgcctacg
     9841 agtgtgtgtg catcagatac aactagaata atagcatcac aagttcaagg ggcagttacc
     9901 tttaataatc agaatggatg ttcacaagta cattcagacc aacaattaac tatggatgtt
     9961 atggctggtt atgcagcaaa tccaaatgta tatggtgtaa tagttgtatc tttgggatgc
    10021 gaaaattgcc aaaatgattt ggtagttgat gcaataaaag agagaacaaa taagccaata
    10081 aaaactcttg taatacaaga agaacatgga actttaaaaa ctatagaaaa agctgttcgt
    10141 tatgctagag aaatggcaca agaagcttct ttgcttagaa aagaagaatt tccaatatct
    10201 gaacttatac taggaactga atgtggtggg tctgacccaa caagtggatt agcagcaaat
    10261 ccattaatag gtgaattaag tgataaactt gtcgatttag gagcaacatc aattttaagt
    10321 gaaactactg aatttatagg tgcagagcat atactagcta ggagagcagt aaatgaagaa
    10381 gtaaaagaaa aaatattaca tatagtacat agatatgaaa attcattaaa attggttgga
    10441 gaagaagtta gagaaggtaa tccatctcct ggaaatatag ctggtggttt aacaagttta
    10501 gaagaaaaat cacttggttg tatacataaa ggtggtcata gacaaataag tgaagtatat
    10561 gattatgcca aacaaattga taagaaaggt cttgttataa tggatacccc tggaaatgat
    10621 gcttcatctg ttgctggtat ggtagcagga ggagcacaaa tagtggtatt tagtacagga
    10681 agaggaacac catcaggaaa tccaatagca ccagtaataa aaataactgg aaataaaata
    10741 acttttgcaa atatggaaga taatatggat tttgatgcta gtccagttat ctatggtcca
    10801 caaacaatgg aagaattaac agatgatttg ttaaatatgg ttgtagatgt agcaaatgga
    10861 aaacaatcaa aagcagagtc ccttggatat actgaaatgg caatagctag agtatgcaac
    10921 tatgtataat ctttaagtaa gaaaataaat acatagaatt ttaataaagg gtgagattat
    10981 gaagtttgta agttttactg aaggagaaaa tgaatgtaaa agaactggag ttttttctaa
    11041 agatgaatta tttattatag atgttaactc tttaaatctc agtagaaatt ttaaggattt
    11101 aaatgaatta attcaaaatg tatctagtga agatattatt aagttgaaaa ctattataaa
    11161 tgaaagtaca ttctataatt atgatgtttt taaaagagag agatgtacgg tacatgttcc
    11221 tatagaaaaa ccaatacatg atatcttatg tgtgggagtg aactataaag accatcttga
    11281 agaaactcaa actcactttg atgatagttt tgaagaacca caaaaaacgg tttattttac
    11341 aaaacgtgtg acaaaggcaa taggtccaga agatgagata gatggtcatt ttgagataaa
    11401 taatcaacta gactatgaag ttgaacttgg agtagtaata ggtaaaacag gtgttaatat
    11461 tagaaaagaa gaggtagaag attatatctt tggttacact gttataaatg atatttctgc
    11521 tagaaagttg caaaaagaac atgttcaatg gtttagagga aaaggattag atacatttac
    11581 ctctttaggt ccatgtattt tacataaaag tgcagttcct tttcctataa aacttaaagt
    11641 atgtagtagg gtaaatggtg aagaaagaca aagttctaat acaggattat tcatatcaga
    11701 cgtttcagac atagtttcag aactttcaaa aggtatgacc ttagaggctg gagacattat
    11761 agctactgga acaccttctg gagtagggat gggattttca cctcctcgat atatgagtag
    11821 tggagatgta gttgaatgtg agattgagag tattggaatt ttaaaaaatt acattaagta
    11881 actgttttaa tataattaat attaaagcta aatctgatgt attaatgaac atattaaaat
    11941 aaattttagg agtttaaatt atgaaagcag aatttttgac accagttgtt acaatatttg
    12001 atgaagaagg aaatttagat aaagaaggta ataaaaaagt gtatgaccat cttatagatg
    12061 gaggtgtaga tggactagtc ataatgggaa gtactggaga gttctttaat atgtctatgg
    12121 atatgcaaaa agaacttata gatttagtaa ctagtcatgt aaataagaga gtaaaagtgt
    12181 ttataggtac aagtcgtatg tgtataaatg aatctataga actttcaaac tatgcacata
    12241 atagtggagc agatggtgtt atgataataa gcccttatta tttctctctt gatgatgata
    12301 gtattgagtt attttatagt gaaatagcta agaatacaga ggctgaaata tatttgtaca
    12361 attatccaga tagaactggt tatgatttaa gtccagaact tacattaaga cttgcaagaa
    12421 aacataaaaa catagttgga tataaggata cagtaacttt gatgggacat accagagatt
    12481 taataaatac tatgaaaata gagttcccag aatttaaggt ttatagtggt tatgatgaaa
    12541 actttataca taatctaatg tctgggggag caggttgtat aggggggtta tcaaatttaa
    12601 ttccagaagt ttgctccaaa tgggctgaag cttacaataa tagagatatg gagaaggtta
    12661 tagaaattca aaaatatatt aataaagcaa tggattttta cttaataacc aatccattta
    12721 taccagcaat gaaaaaagct atgaatatca gagggttaca aataagtgat tatatgactg
    12781 ctccatttgt aaaaccaaat gaaaaacaag tatgtgaaat taaaaaattg atgaatgaat
    12841 taaatatatg ttaaatcaac cttgcaaaga ggtcaacaat aaattacaat tatcttaata
    12901 aaaattaaat taaaataata ctagggtact atattagttt aatagggaaa gtgatgaaaa
    12961 ttcactacag cccccgctac tgtatacgga tacgaaatca aaatccactg aaatttataa
    13021 aaattttggg aagggtgatg gaggaaaagc cgtgagtcag gagacctgcc cagtattata
    13081 taagttcaat ttagggtgga gattgtaatt tataataaaa tgaagtatca attcgttttt
    13141 gtaaagttga tatatgaact tctccttatt aaattaggag aagtttttta ttttattaaa
    13201 aggttatact tattaaagat gactttagga taactttaat aagtatactt taaaaagcat
    13261 atattattaa agttttttag ccactcataa aaaataagat attggagtga aagagatgaa
    13321 atttaaatca aatgttttaa agcagttttt tttaattgct atggtaattt ttatggtagg
    13381 aggtcttata gggtgtagta aaagtgacaa agagaataaa caagctagta ctggtaagaa
    13441 attagaatta aaatatgcta gtggatttag tgttgaagat ttagaagatg gaattaaaaa
    13501 agtaactgat ggagataatc gagaactgat tttaatccca aagaagatta aaattcctga
    13561 aaaatataaa aatgctaata tagttagaac gcctgtggac aatgtattaa tagcatctac
    13621 aacacaggct tgttctctga gaacaataga tgaactagat agtataaaag ccgtaacaac
    13681 agaagagcag tactggacta ttaaagaaat taaagattta atgaaagatg gaaaaataca
    13741 ttacgttggt tcaaatttag caccagatta tgaaaaaata gtagacttaa atcctgactt
    13801 aactatagtg agtagtggtt tatcagaggc tgatacaaaa tttattgaca aattaaaaga
    13861 attagggctt aattatgttg tagacaatga gtataaagag caaaatccat ttggaagaat
    13921 ggagtggaca agtttaatag ctgcatttta tgataaagaa gatatggcta ctagtgaact
    13981 aaataaaact gtacaaaagg tagaagaagt aagtaaaaaa ataaaaaaca aagctaaacc
    14041 taaagttgta tgggcatctg tatatgaagg aagtgcttat atagctccta aagattcata
    14101 tgtggataat atgataaaaa tggctggtgg aataaatgca tgtgcttcaa tagattcaaa
    14161 tgaagggcga gtaagtattg aacaattgca tgaagttgca aaagatgcag atatatttgt
    14221 atactcatca agttcagatt atgcaccaaa tttagattct ataaaatcta gtgcacctgt
    14281 attggctgat ttggatgttg taaaaaacgg gaacctatgg gtgtttgctc cagattatta
    14341 tcaatcagta gataagacgg acgaattaat agtagattta gttgagatgt tccacccagg
    14401 tactacaggt gaaaaaatca aacattatat aaattatgat aaatagtaca gagaggacac
    14461 aaaaagatag gttttataga ttttagttaa tatagctttg ctaagaggag tatacaaatg
    14521 gattttatca ataaaatgag aataaggaat acaacaatat ttatagtatt aattataagt
    14581 atgatactta cttttttagt gtgtgtagca tttggctctg taaaaattga atttaaagaa
    14641 gtaatagatg ttgtattaag aggtatggat aatacagaac attataaaat aatatacaat
    14701 ataagaatcc ctagagtact tgcaaccatg gtaggtggag catgcctagc agtatcaggt
    14761 attttattac agatattttt taaaaaccca atagttgaac cgtatatatt aggagtatct
    14821 tcaggttcta ctttatcagt agctctcatg attttgggag gatacacatt gggatttgag
    14881 agtgtaaatt caatgagtat atttgtagca gcttttatag gagctttaat tgtaatgtct
    14941 atagtcattt tatttgcaaa taaggtaaag aatataacaa ctcttttaat aataggaatt
    15001 atgaccggat atgtatgtag tggtataaca aatgtgttac aatcatttgc aaatagtgat
    15061 aaattaaaag aatttactat gtggtctatg ggaagttttt ctggatttac atgggaaaag
    15121 gtagaaatac ttataatagt tggtatatta ggtcttttga tgactgtatt cataataaaa
    15181 cctttaaatg cttttttact aggagaagag tatgcaaaaa gtattggcat aaatataaga
    15241 ttttttagag ttttgatagt atttatatct agtatattag cagcaatagt atcagcattt
    15301 gcaggaccta tagggtttat tggacttgca gtgccacaaa tttcaaaaat gatatttaaa
    15361 acatcagata atagagtttt gataccagta gtaatatttt taggcagtat agttacagct
    15421 ctttgtgatt taatatcaag agtaatttta tcaccagtag aattaccaat aagtacgata
    15481 acatctttat taggagctcc aattgtagta atgttattgg tgaaaaggaa ggatagggct
    15541 tagacatgtt gaaaactaat aatttaagtg taggatacaa taacaaagtt gttataagta
    15601 atataaatgt agaagtaaaa aatggagaaa tactatgtct tttgggaagt aatggagctg
    15661 gaaaaactac cctattaagg tccttatcta aacttatatc accaataaaa ggtgaaatat
    15721 atttgaatgg tgtaaacata aactgtatat caagaaaagc attatcaaaa aaaatggctt
    15781 tagtacttac aaatagatta ttaggtgatt taatgactgt acaagatata gtcaatatag
    15841 gaaggtatcc atatacagga ttttttggga gtctatctaa gaaagattta ataatggtag
    15901 atgaagcatt ggagtctgta gatgccttac atttaaagaa aagatatttt gatgaactaa
    15961 gtgatggaga aaaacaaaag gtgttggttg caagggctct tgttcaagaa ccagaaataa
    16021 ttatattaga tgagcctaca actcacttgg atataaaaca tagattggaa ctcataaaca
    16081 tactgaaaaa attaagtaaa gaaaagtcaa tatcagtaat actttcattg catgaaatag
    16141 acatagcatt aaaaagttgt gataaagttg ctcttataaa aaataataaa gtcatagctt
    16201 atggtcagcc tgaagatgta gtagatgagg atattataaa ttcgctttat gaattggatg
    16261 ataaaaactt taatagtctt ttaggttcag tagaaattag taataaatct aaaaatgaag
    16321 tttttataat tggtggtggg ggtaaagcaa caccaattta tagagctttt actaaaaaag
    16381 gtataggtct gtattctgga ataatacatg aaaatgatat tgattatgaa attggtagaa
    16441 ctatggggat aaagatgttt acagaaaatc catttgagcc tataagtgat gagagtttca
    16501 atttggctat tagaaattta aatgattcca agattataat agatacagga tttagtgtag
    16561 gagagacaaa taagaggaat atagatatta taaaagaggc tttaaaattg gataaaaaag
    16621 tttattcttt tagaaataga gatgagagta agaagtatta tgacagtttg gatagtaaga
    16681 tagaacatat agataaagtt tctcaaataa tcaatagtgc agatataaat aatttattat
    16741 aatttatgaa ataaaatgta cttatgtgct atagaaaaaa gagtatagat tataattttt
    16801 ttgaaattac aatctatact cttttgttat tttgtttatg tatttatata tttataaaac
    16861 tttagcattt tatagataac caatagcttc ctctctagtt aaaaaaaagt gaatccctgt
    16921 agtcgattct ttccatctat tttcattaaa atcttcagct acaatcgttt ctcctactct
    16981 atatataaaa ttttcatcag catatgcacg agcactttta aaagattctt taccatctat
    17041 actagtaatt gacagtactt ttgccttatc acatctacaa gcgtttgatg tagcagacac
    17101 tctttttgcg tcgctgggta ttaataattg taccattcta aaattaaaac attttttata
    17161 gcctataaat gctcctgttt caggacaata caatttaaaa aaacgggtac tttcatctga
    17221 tattactcca tttaaagtag catgttctaa atttgaattt tctaaattag ctcctctaat
    17281 atctgagtta gataagtcac aaaatctaaa acttgcacca cttaagtctg cattttttaa
    17341 tatagaattt tttaatgaac aatccgaaag ataagaatta ttaaaaatag agttttctaa
    17401 atctgctttt tctaaattta aattgataag gtcagaccac gaaaagtcaa tattacttaa
    17461 gcagtatcct cttaagtcca tattttctaa tcttaattct tttaaatctg ccttttctcc
    17521 agcattattg tttttaagcc ataaataatg tttctcaagt gttttttcta attcttgttt
    17581 agaaatagtt ttcattaaaa tcaccttcat aaatttattt
//
LOCUS       DBGJFU010000082        17372 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.082, whole genome shotgun sequence.
ACCESSION   DBGJFU010000082 DBGJFU010000000
VERSION     DBGJFU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.082"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     regulatory      22..107
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            231..6194
                     /locus_tag="ACOIAS_002534"
     CDS             231..6194
                     /locus_tag="ACOIAS_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HGZ2702238.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            6398..8278
                     /locus_tag="ACOIAS_002535"
     CDS             6398..8278
                     /locus_tag="ACOIAS_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HGZ2702239.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            8645..10246
                     /locus_tag="ACOIAS_002536"
     CDS             8645..10246
                     /locus_tag="ACOIAS_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HGZ2702240.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            10465..12063
                     /locus_tag="ACOIAS_002537"
     CDS             10465..12063
                     /locus_tag="ACOIAS_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2702241.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            12353..14551
                     /gene="slpA"
                     /locus_tag="ACOIAS_002538"
     CDS             12353..14551
                     /gene="slpA"
                     /locus_tag="ACOIAS_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HGZ2702242.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASSSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            14797..17139
                     /gene="secA"
                     /locus_tag="ACOIAS_002539"
     CDS             14797..17139
                     /gene="secA"
                     /locus_tag="ACOIAS_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGZ2702243.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
BASE COUNT         7194 a         1928 c         3316 g         4934 t
ORIGIN      
        1 aaaatgggta agaaagtagt gaaaattaaa tggcaaaatt agggaaatct aatgacgcaa
       61 aactataggg attaaagctt ttaaagccat atcagccagt tgctaaaaag agtaaagtct
      121 ttttatttta taattaagat tctctttgag tgtctttttt tattgtctga aacttatagt
      181 atttaaattc atgaaaaatt agatatttat taagggagga ttgttatttc atgaaaaagg
      241 caatatcttg tgtactagca gtatctatgt gtagtgcacc actaaacgtt tttgcagaac
      301 ctatattaga ggggaagcta agggcagttg aaaaatcagt tacagaaaag ttaaggggga
      361 gtcttgaagt agatttaaat ttttcactgc caataaaaaa tagcgagtca gaaagtatga
      421 ctaatatgca gttacgatta aaagatgata gtaacaatag tggaataata aagcttggag
      481 aaaaagattc aggaacagta aatgttggga cagataatat agaatatact ataaaaaaat
      541 tatctgcaag tagaagtgaa ataaaaaata aagatgagaa tatatcatat tataatattg
      601 tatttaataa tttacctgtt ggaaaataca atgtagaagt ctctggagat ggatttaaga
      661 acaaagaaat taaagacatt gatatatcaa gttattcaca aagagtctta ttaagcaata
      721 gagcaagtgt agataataaa aataaacctt taaattatga attgttctta atgggtgatg
      781 taaatggaga cggtaaagta gataagagtg attataataa ggttttagag aatatagatt
      841 caaataaaag agaatttgat ttaaatagag atggaaaggt agatattgtt gacttagatt
      901 atgtacagaa aaatttagga caaagtgaag atgcaaaaag cctagaaagt atagtatcta
      961 ccaatcctat agtagataca agtaaggttg aattaaaagg aaatagtgat gtagatatta
     1021 atggtaatgt agaaaattta tttagtggag aaaataatgg agttacaata tcttcaaaaa
     1081 atgatgaaat atctgaggaa aaaccagcag taatggatat agctttatca gaagcactta
     1141 atatggaagt catcaatata aaagctccag aaggtactgc tccagcaaaa ggaatagttg
     1201 aggttactga caaagatggt aatgttcaac agattcctta tgaaaacaaa gaatatatga
     1261 aaaacttaaa agatgaggag caacaagagg aagtaaataa cgaacaagaa gaagtaaatc
     1321 aagaagaagt aaataataaa gatgaaaata taaaccagac tgataataac tcaaataata
     1381 aagatgaaaa taatgataaa caacaggaaa atagcgataa acaagaagca aactctaata
     1441 ccaatcaaga taaaaataat gataaagaaa attcatcaat ggataaacct aattcagatg
     1501 aagatactcc aaaagaagag ggtaatatca gtaaagatga taactctaca acatctacat
     1561 atagtaggtc agcattagaa acatatgcag atgttcaaaa tagtgatata gtaataaatc
     1621 ttggtaaaca agtagcagta aaaaagataa ctataaaaat aacagctaca acagcaaacg
     1681 atagaaatct tgcagaaata tcaaaagttg aatttttaaa caatgtatat aaagaaatac
     1741 caaagccaga gatgaacata cctaaaataa ataaaatatc gactagcact gctgttggaa
     1801 atgaaaaagt atctttttat tggaataatg aagtaaatgt atcagcatac gaacttatac
     1861 tagacaaaat tgatagtaag ggaaaagtat tatctactaa aaaattacaa actagtaaaa
     1921 atacaataga agttagagat atagaagcat atggactata tagagttagc atacaatctt
     1981 taaatggaga ttggtctagt ggttataaag atgcaacacc agaaaactta gacggagtac
     2041 cagaaaatgt agattcaaac tataaaccta tacaatttag cccagatagt atattagaat
     2101 ttcaagttgt tccagacaca aaaccagaac caccagaagg tataaatgtt aaaggtaaat
     2161 ttaaatctct tgatgtaagc tggaaaaatc ataaacaagc taaagatttt gacttgtact
     2221 ataaagaaaa aggctctaat gggtcttgga taaaatacaa tgataagctt attcgtggga
     2281 caagttcaaa gatagaaggt cttaaggata atacaacata tgaagtcaga atgacagcta
     2341 ctaaccactt gggaactagt ggaatgtcta aaacatatat aggtacaact gaaagcatgg
     2401 atgcaccaac aacacctaac tatagattaa ttaatactcc taaagaaaat ggtggatttg
     2461 gaataccaac aaatcacatt gtagatgttg tttatccaga tggatatgac aaaagtgaat
     2521 atagtgataa aaatccattt gataaattca atgtagtaga tggggacttt acaacacatt
     2581 ggacattccc aacatataat gcagctgagg gaacgaatag aggaccaata ataacttttg
     2641 ataaatctta tactatggat acttttatgg ctacacctcg ttttgatgtt tctgataaga
     2701 caataaatga ctttgaggta cgatattggg atgaaaatgg tcaattacac aatctggggg
     2761 atgttccttt tgaaacaaag acagaccctg taaataataa taagtatgta atggttagat
     2821 taaaagaacc aataactgct accaagatac aagtaagtgt ttgttcatcc tattcatata
     2881 agtctaatat atcagaactt aagttttatg agtatgacag tatagaagat gatgtaagaa
     2941 atctatttat ggatggccta caagttgaat taaaagaaga tgtaactcaa gaaaaaataa
     3001 ctgaattaaa agatagatta aatactccag ataaagctag tggagaatat catccatata
     3061 agacagttat tgagagagaa ttaaaattag ctcaagactt atataatgat agagccacac
     3121 ttactgatgt aactactgta aatcaagaaa taaataaatt aggaaaatat aaagataaaa
     3181 acggacagta tactgtaaat tccaataatt taggtatgca aaatgactgg caggcattag
     3241 gagtagctgc tagagcagga gatgaaatca cagtatatgt tggaagtaaa agtggaaaaa
     3301 ctcctaagtt gatatataca cagtactatg gagaatctgg tgcatataag agtggagaaa
     3361 taaatcttaa cactggaaaa aatgtaattc aattaagcaa acttcatagt ttagatatag
     3421 aatgtggagg aagtttatac ataagatacg ctgaagatac tccaagtgga ggagatataa
     3481 aagtacgtgt agcaggagct actaagatac cacatcttaa tctaaatgga acaataaatg
     3541 ataagagtcc tgcgggagtt tctgaatcta agaaaaagat aaaagcatat atagaagaat
     3601 tagctaaata taaagatgct gtaaaaggag aaccatatta tccaggaaca aatgttggta
     3661 attcatatgg atattcagaa aaaactggtg tgttaaacac tacagatatt gaaagtgata
     3721 aagtaacatt aaatgttcct gcaacagcag tatatcaaga gatatcaaaa ggaaattctg
     3781 atttaaatac acaagtagat agattatata atagtatgct tgcatgggaa caaataatgg
     3841 atttagttta ctcagaaaga ggagtattta aaacacaaga tttagatggt aatggaactg
     3901 tagatgataa tgaatttaac ttaactaaaa atgatatggc tccaaaatct cgtatgaaca
     3961 taaaatacca aagaatgttt attggagcat ttatgtatgc ttctggatta catgtaggag
     4021 tagaatatgg ttcagttcca gggcttatga atggagtacc tttccaaata gatgatagtg
     4081 gaaaagctac aggaggaaac ttatttggat ggggaatagg tcatgagata ggacatgtta
     4141 cagatattgg aaagatgaca tatagcgaaa ctagtaacaa tgtacttgct ttacttgctc
     4201 agacctttga tgataagaca cattcaagac ttgaaggttc aacaatggat aagatatatg
     4261 agcatgtaac ttcaaatagc ttaggtatac caagcaatgt atttgaaagg cttggaatgt
     4321 tatggcaatt acatctagct tatgatgatg attttacggg ttcaatgtta aagaataact
     4381 cagatgctga tttaagtaat gatacattct atgcaaagat ttcaagaaaa tacagagcac
     4441 tttcaagtag tgacccaatt aatagtttac caaaagacca aatgctagta gcaatggcat
     4501 caagtgttgt tgaaaaagat ttaagagatt actttaaggc atggggagta gaaattacac
     4561 cagaattaaa cagtataatg gatagcaaga actacgaaaa agaatcaaga aagatacaat
     4621 atgtaaatga tgaagctaga agaaagattt taaatgaaaa tatagctagt atggcaaaag
     4681 atactaaagt aagtgctagt ttttctgatg gaatacaaag tggttctcta gttaaatcta
     4741 ataaaatatc tatagaccta gatgtagata aggataaaga taagatacta ggatacgaaa
     4801 taataagaag tgatggaaat tatttagatg gtgaaaatgg aacacaggta aaatacagaa
     4861 cagttggatt tgtggatgca aaaggaaata gcaagactaa atttactgat aatatatctc
     4921 ctcttaataa cagagcattt acatataagg tagttgcata tgattatcac ctaaatccaa
     4981 cagaagaatt tgaagttggt actgtaaaat tatcagatga agggaaaata aataaatcag
     5041 catggtcatt cataacaaat actgtttctg acggtgatgt tagaactgaa aatgactcac
     5101 atggaccatt acaaaatcct gaaattgata atataaaaga taatgatgca agtactacat
     5161 ataaaggaaa gataataagt aaagcagagt ggaacaaaga ccctcaaaaa gaccctgata
     5221 taaatgtaga agaaaatcca tatataatag tagacatgaa agaaacatta cctatagtag
     5281 gactaaaata tacaaaacca gagacagaag ctagaaagtt ctcattaaaa ggattattta
     5341 actttagaaa gaatactagc acaacataca atcctttaac taattacaag atacaagtaa
     5401 gtaatgataa aaagaactgg gaagatgtaa gtcaaggaac ttttgaatat ggacaagatt
     5461 tagttggtgg aggaaaagac caagataatg aagctagagt tacatttaat aaagatggta
     5521 aactttggac ataccaagct agatatgtaa aactaatctc aaataataag tctaatatag
     5581 aagttgcaga attagatata ataggacctc caggtgataa tatagaaatt ggcataataa
     5641 ataatgcaga cgaaactcgt actaatggaa ttggaaaatt agcagaagat tatgtatatc
     5701 aaagtgatga ctctagcaca agtgaagatg aaacaggtaa gattccagcg ggttcaatag
     5761 ttattacagg tgaatacagt ggaaatccag catttaattt accactactt atagataaaa
     5821 acaataagac tatatctgga gaagcacttt tatttgcaga agttccagaa aaaggtgaac
     5881 taggtgaaat cagtaaaggt acatgggttt actatatgcc aaaagaaaac tttgattctt
     5941 taagtgataa ggttaaggct gaactatata gatacaatga ccttcaagga gatactccag
     6001 taggtcaaag atttgtaagt gatacaatgt atgtacctgt aggagctaag aattatgatg
     6061 agttaaagac aataagtctt actgatagta atagtaaagc tagaaaagct acaagaaatt
     6121 ctattgatat cagtaataag aaggttataa ctgtaagcaa taaagtaaag ggtaatattg
     6181 ttagagataa atagtaggtg gaactctaaa aacgtaaaaa tactaacatt gtatatctaa
     6241 taagattagg tactagaata gttttaaaat agtagatatt aaaagtaaag tatataaatg
     6301 taaccataaa tttagggtaa tttataaatt aaatataaaa gctaggtgga taagtaaatt
     6361 tatccactta gttaatgata gataaggacg ggtgttaatg agtaaaagaa aatcttttat
     6421 aagaactata gcagtatcaa ctatggcagt agctgtaact ggtagtgcta catgtgcata
     6481 tgcagctcca gttttacaag gaacaaaaac gtatgagaaa gtaaatacta tagatatatc
     6541 tgtggactca gtagaaaaca tagtatattc atttcaagcc agtataaaag tacaaggtga
     6601 ggttgaagta gtagataatg agcagaaaga aaagattact tggtctgata atataaaatc
     6661 tcagataaaa agtggaaatg caaatgctac ttgtagagta gaatacaaca aatcaagtaa
     6721 tacaactaca ttagatatat atgttacatc aaatgaagat ttactagacg gaaatagatt
     6781 gaatatagga agaatatctg taaaaaaatc tggcagtaac tcaaatgctg actataaagt
     6841 tttaggaaaa ggaacaaatg ataaaccagc tttaaaaatt gtaacatata acaacaaaac
     6901 agtggattat gaaaatataa gctctgatga aggattaata tttaccctta ttaatgagag
     6961 tgaagtaaaa ccaattggtg gaactggttc tagtaaaaat gaccctgaaa agtataaggt
     7021 agagaaaagt gaagcacttg aatatttatt aaacaatatt aggataaact attctattgt
     7081 tagtaaagaa acacaagaaa gtggttcaaa tgtaatatta aaacttggat tagcacaaaa
     7141 aacaactaag ggaagaaaag ctacaataaa caaatatgtt gaagttacac ttccaaaaag
     7201 tttagaatac atagttgaaa atgaattatc aaaaccggat gaattaccac cagataatgg
     7261 caatggtgga aataatggtg gaggaagtaa ttcaggcggc tcatctagtg gtggaagctc
     7321 tggaggagga agttcaagtg attctacatc taatgtcaca gtcaaaaaat taaaaggtgc
     7381 tgatagattt gagacagcta tcaaaatatc tcaaagtggt tggacaaaat cagatacagt
     7441 tgtaatagta aatggagaag ataaaagtat ggtggatggg cttacagcta caccacttgc
     7501 tagtgtaaaa aactcaccaa ttcttttatc atcaaatgaa aagcttcctc aaaaaactgt
     7561 agaagaattg aaaaggctaa atccatcaaa agtaattgtg ataggtggaa acaactcaat
     7621 gccaaattca gttgttgaag ctatcaaagc tgtaaattca aaaatatcag tacaaagaat
     7681 aggtggagat acaagatatc aaacttctat taatatagca aaagaaatag ataagacgaa
     7741 taatgtaagc aagttgtata taggagcagg aaatggagaa gctgattcat tatcaatagc
     7801 atcactggct ggtaaagaaa aaactccaat agtacttaca caaaaagatg gtgtagataa
     7861 tgaagctgaa caatttatta aatcaaataa ggtgtcaaat atatatttta ttggtggagt
     7921 agaaaaaata tctaacaagg ctattgaaca agttggaaaa atagcaaaca aagatatatc
     7981 aaacaatagg gtggcaggtc aaactagaca agagacgaat gccaaagtaa tagataaatt
     8041 ctactctcaa tctaagctag atggagttgt agttgcaaat caagataaac ttatagatgc
     8101 tttagcagtt ggaccattag cagctaaaaa caattcacca gttatacttg ctacaaatac
     8161 gttagataaa tcacaagaat cgtcattaaa aggtaaaaat tcatcaaagt tgtttgaagt
     8221 aggtggagga atagcctcat ctgtaataga caagattaag agtttaattg aaaaataaat
     8281 attaagtaaa tagaaaataa gaatgtattt agatataaag ttatatattt aaatgcattc
     8341 ttattttatt ttcattatct taacaatcta ataatattga ggttaaaatg tataataatt
     8401 tgagctatat taagtttaat tttaaataaa gcagatatct tttatatata taaatataaa
     8461 ttcaattttg aatatgttta aaaagaagtt aaaaataatt ttttaaattt aataaaataa
     8521 aacactaata tatagacgag atatattcat atcatatata ctctataata tatggaaaat
     8581 aaagagaaaa taaatggaaa ataatcacaa tataaatata aaatataaaa atcatcaggg
     8641 ggatatgaaa atgaacaaaa aaatattatc attaggtcta gcagtatcat taatcttagt
     8701 aaacttcaaa agtgtaaatg catcatcagt agtagaaaaa atatacggta aagatagata
     8761 tgaaacagca gcaaagatag ctgataaaca gacttatgaa acagtaattt tagtaaatac
     8821 agaaaaatca cttgcagatg gattaagtgc aagtggatta tcgggagcta caaaagctcc
     8881 aatactattt acacaacaaa ataagatacc agcagacaca aatagatgtc taaaaaatat
     8941 caaaaaagca tatataatag gtacagaaga tactataagt aaatcagtag aaaaagaact
     9001 agattctaaa aatatagaag tcaaaagaat tggtggagaa gatagactaa aaacaagcta
     9061 tttaatagct aaagaaatag caactataaa aaaagtggat aaagtactat taactaatgc
     9121 atatagtgga gaagcagatg caatgagtgt atcatcagta gctactagag atggagctcc
     9181 aatcatactt acagatggaa agagtgtgcc ttttgatgta aaaaatattc aatcatattg
     9241 tataggttca gaggagataa tgagtaatcc tctagttaaa aatacaaact cagtaagaat
     9301 agaaggaact gaccgatttg aaactaacaa aaacgtaata gactactttt ttaatagtgc
     9361 agatggattc tatgtatcag atggatacca attagtagat gcaatagcag ctgcaccact
     9421 tactaaaaac tctccaatgg tactagtaaa tgatggaagc gataaaactg tattagaagg
     9481 agctaagaat ataacctctg taggtgaaat aaatgagaag gtaatacaac agtgtataaa
     9541 tgcttcaaat tcaaatgggc aacccccaac aattacagtt ggaagtacag aggtatataa
     9601 gggtgaaaag tttgacactg gcaagttaaa tatagtagct aaagataaca cagggaaggt
     9661 attaccaata gaagttgatg ggtttataga tacaaataga gtaggtacat acatattgac
     9721 attaaaagct acagacgaat ggggaaaaag cacaggaaaa agagtagaaa taaaagtgtt
     9781 ggatgacaaa tctcatgatt acaacagccc agaatttaaa aaaatggtat ctactgaaat
     9841 gtataactta atcaattcct atagaaaaga aaaaggcaaa gagcctttgc tagtgtctag
     9901 taggctagaa ggtatggcaa atgcatggtc taagtatatg atggataaaa aagtatttgc
     9961 acattatata gatggaaaaa atgctccaca agtattttct gaatttggaa tgagaagtga
    10021 ggaaaatata gcatatattt atatagattc taagaacgtt caaacgacac aagatgcaaa
    10081 agacttggca aaggctatat ttgaggtgtg gaagaagtct ccagagtaca atgcaaacat
    10141 gcttagtaat gagttttata gtactggatt tgggctttat atattatctg atggtcaagt
    10201 gcatgctact caggagtttt taaatggaaa tgaaggtagc ttgtaaaaaa ttccataaat
    10261 cttgtttttt tgttaattta ctgatattat aaaatgaaaa tcacatctaa agtacttttt
    10321 gtaatctgtt ttgttattgc tttacaggct atggtatttt aggtgttttt tgttttagta
    10381 gtttatagat tttgagggaa tcactactga ggatgtaatt tatgattagc tgaattatat
    10441 aaaaaaatta gtaggaggag tttgatgaag gtaaataaga gagtattatc aataggattg
    10501 acaatatcat taataatggc aggagcacca aatatcaatg cactttcgag catagaaaag
    10561 atacagggga aagatagata tgagacttct gcattgatag cagataaaca aatatatgat
    10621 acaatcatat tagtaaatac tgataattct atagtagatg gattaagtgc aagtggatta
    10681 tctggagttg caaaagctcc tataatgttg gtgcaaagag ataagatacc aacagatgtt
    10741 gaaaaaagac taaaggatgt taagaatgct tatgttatag gtacagagga tacaataggt
    10801 aaatctgtag aaaatcaact taagaataaa gggattgaag ttaaaagaat tggtggggaa
    10861 gatagaataa agactagcta tcttatagct aaggagattt cagctattaa accagtaaat
    10921 gatggtgata aggtttttct tgtaaatgga tacacaggtg aggctgatgc aatgagtgta
    10981 tcttctgtag ctgctagaga tggagttcct gtgatactta ctgatggaaa gagtatacca
    11041 tttaaagttg atggcgttca gtgctattct cttgggtcag aggagataat gagtaatgaa
    11101 cttgtgagta agactaattc agttaggatt gctggtaagg ataggtttga aactaataag
    11161 aaggttatac aacgctttta taaaggaaca aagaagtttt atgtttctca agggtataag
    11221 ttagttgatg ctgtggctgg ttctccgttg gctaaggaca aacctatagt acttgttaat
    11281 gatggaagtg ataagagtgt gcttagaggt gctgatgaag ttacttcttt aggtggaatg
    11341 gataagaagg ttgttgacca atgtattagt tctgcatctg ataaaaatac aatgcctaca
    11401 attactgcta atgatgtaga gatttctgta ggggataagt ttgataatag tatgttgaat
    11461 atagttgcta ctgattatta tggaaatgat ttaaaggcaa atattaaagg aaatgttgat
    11521 attaacaagg ctggaacata tgttttaaat ataagtgtag tggataattt aggtcagaag
    11581 agtgagatta gtgtaaatgt taaggtagtt gtaaatgcga gtacaaaaga ctctaatagt
    11641 tatgagttta aggctatggt atctaatgag atgtatgatt tagttaattc atatagaaaa
    11701 gaaaaaggca agaagagttt aagagagtta gactctttag ctggaatggc aaatgcatgg
    11761 tctaaatata tggaagataa gaaggtattt gcccatgaga ttgatggtaa aaatgcagct
    11821 gaagtatttt ttggctttgg agcaagaagt ggtgaaaata ttgcatatct accaatgaat
    11881 gttaaatctg tttatacttc taaagatgca aaagaaatgg ctgagtctat ttttgattta
    11941 tggaagaagt cttctaagta taatgaaaat atgttaaaag aagaatttta tagctttgga
    12001 tttggtatgc atgtttctag taaaggagaa gtgaatgcga caatggaatt tttaaatagt
    12061 tagattttat attaatttac attggaaaag atatagtaaa tttgtaaaaa taaatatata
    12121 aaattaaaat aacttataaa attataaaaa ataatttaaa aataataaaa atgatgaaaa
    12181 aacaattaat ttgttggaaa aaacttcaat taattttgca taaattattg aatttatatt
    12241 ttatatagtc tatcataaga gtgtgagaaa tatttggatt atatgaacat ggaaatttaa
    12301 tgtacataat taaagagatg taattaatat aatgttggga ggaatttaag aaatgaataa
    12361 gaaaaattta gcaatggcaa tggcagctgt tactgttgta ggttctgctg ctccgatatt
    12421 tgctgatact actgttaaag aagaaggata tacggtagta caagacaaat atgaaaaatt
    12481 acttaaagaa ttaaaggcaa agataaaaga tggaactatt acatcagttg gtgttgaatt
    12541 tgatggaaaa ccaattacta cattagcacc aaaggcagat ggtagtgata aagatgcaat
    12601 agcagaacag ttagaaactc ttactaaaaa ccaacttaaa ggtttaggtg atggaaaata
    12661 tgtagatttt aaaattactt atggtgctaa agcagaagtt ccagctgctt cattaagtgc
    12721 agatgatata caaaaatatg cagatcaaat aaatgcttct gagaaaatat tagttgaagt
    12781 agcagcagga tctgaagcag gtatagcaaa atttgattct gttaacaata aagttatagc
    12841 aggagatgct cctcttaaag ttaaagatgc agtgaaagcg actgttacta ctaatggtag
    12901 taataaaaaa gttcttacaa tatcagctgc agctggatta tcaggatttt catatggaac
    12961 acttaaagac acaggtgcaa gttcatctga tgttgatgca ataactcttg acactacaaa
    13021 tgcaacaata acagaaggtg atactaaagt attagatttt gataattcat ttaagtttaa
    13081 cgaaagtact aaaaaagttg gtagtttagt tacaccaaat acaacaaaca ctccagcaga
    13141 tcctggtact aaaactactg ttagagtgat aaaagctgtt gaaaaaacaa tagatgtaag
    13201 ttcaaattca actacaaaag ctaaagattt agctaaacaa tatgtattca cagatgttag
    13261 tgatactgat ccagaaagtt taagttatat gcttaaaaac ataaatgatg gaaaagttgc
    13321 tgttaaaaat tctgatggag attatgaagt aactatattc ccagaaggaa aaagattaaa
    13381 tactttatct gcttcttcag ctaaaactat tttaggagat aaagatactc cagcaaaaat
    13441 agttttaaag gcttcaacaa caaagaaatt agcagactat atagatgatt taataactta
    13501 taacaatagt tattcaaatg tacaaactgt agcaggtagt gatagaatag agactgctat
    13561 agagctaagc cgtaagtact ataactctac tgacaaaaat gctttatatg gagatcctgt
    13621 aaataacgtt gtattagtag gttctcaagc tatagttgat ggattagtag catctccttt
    13681 agcagcagaa aaagatgctc ctttattgtt aagttctaaa gataaattag attcatcaac
    13741 aagagctgag ataaaaagag ttatggactt aaattcttca acaggaataa aaaataataa
    13801 agaagttttc atagcaggtg gagtaaattc tatatctaaa gacgttgaaa atgaactaaa
    13861 agatatggga cttaaagtaa ctagattatc aggtgatgat agatatgaaa cttcattagc
    13921 gatagctgat gaaatagata taaatgataa agcttatgtg gtaggtggaa caggattagc
    13981 agatgctatg agtattgctc cagttgcatc tcaaataaaa gatggtgaag ctactccaat
    14041 agtagttgta gatggaaaat ctgacaagtt atctaaagaa gctgaagatt tcttagatga
    14101 tgcacaagta gatataatcg gtggagaaaa tagtgtttct gctaaaatgg aagattatat
    14161 agatgatgct actggaaaat ctccagaaag aattagtggt gctgatagac aagcaactaa
    14221 tgctgaagtt ataaaagaat actttgataa agatggagtt tcaaattact tcttagcaaa
    14281 agatggttca actaaagagg atcagttagt tgatgcatta gcagcagcag cagtagctgg
    14341 aaactacggt tctaagcata atgaagatgg agatataact acagatgctt ctcctgctcc
    14401 aattatttta gcaactgata atttatcagc tgaacaacat gtagctgtaa gtaaaactgc
    14461 tactactaat ggtgctaaga acttagtaca agtaggtcaa ggaatagctg attcagttgt
    14521 tagcaaatta aaagatttat tagatatgta atataaattt tatatattga ataaaaaagg
    14581 tttctctttt gagaaacctt ttttatttta aacataaatt tttataaata aaaagaggct
    14641 aattagcctc tttttaatgt gagaaacact taaaaaataa gatgtaatta tttatttaag
    14701 tatcattatt atactatacc ttaaaataaa tgtctactat tctataattt taaggtataa
    14761 tagtaagtgg aaaataaata aggaggtaaa ttaaatatgt cagttttaga tgtaatatta
    14821 gacaaaacag atgaattaga aattaaaaaa ttaaataata tagtagataa aatagatgct
    14881 ttagaagata aaatacaaat actagatgat gaagcattaa aaaatatgac gaatgtattt
    14941 aaggataagt taagtaaagg agaaacctta gatgacatat tgccagaagc atttgctgtt
    15001 gtaagagaag tatcaaaaag aatattaggt atgcgtcaat atagagttca attaattggt
    15061 gggattgtat tacatcaagg gaaaatagca gagatgagaa ctggagaagg aaagactcta
    15121 gtaggagtag ctccagttta cttgaatgca ttgactggta agggggtaca tgtcatcacg
    15181 gttaatgatt acttggctca acgtgataaa gaacttatgc aaccagtata tgaattttta
    15241 ggtttaacag taggagtaat tattaatggg caacagccag tagaacgaaa attacaatat
    15301 caatgtgata ttacttatgg tacaaacaat gagtatggat tcgattattt aagagataat
    15361 atggtaacgc aaaaatcaga gaaagttcaa agagaactaa actttgctat agttgatgag
    15421 gttgattcaa tacttataga tgaagctaga acaccactta taattgctgg tggaggagat
    15481 gaaggtacta agctgtatga acttgcaaat gcttttataa agaatataag agatgaagat
    15541 tttgaaatgg atagaaaaga taaaaccata gcacttactg caagtggtct tagtaaagct
    15601 gagaaatttt ttggaataga aaatataact catatagata atatagaaat ttttcaccat
    15661 gtaaatcaat ctctaagagc acataaatta atgaacatag atgttgaata tgttgttaga
    15721 gataatgaga tactaattgt agatgaattt acaggaagaa taatggatgg aagaaggttt
    15781 actgatgggc ttcatcaagc aattgaagct aaagagggag taaaaataaa tgatgaatca
    15841 aagaccatgg caacagtaac atttcaaaat ttctttagat tatactctaa attatcagga
    15901 atgacaggta cagcaaagac agaggaaagt gagtttgaag ctacatataa gattaatgtt
    15961 gttcagatac caactaataa accagttata agagctgatt tacatgataa agtattcaaa
    16021 acagaacgtg caaagtacaa tgctgttgta gaagaaataa taaggataaa taaaactaag
    16081 caaccagtgc ttgtaggaac tgtttcaatt gaaaaatctg agaaattatc taagctactt
    16141 aaaaagaaag gtgtaaagca cgaggtctta aatgctaaac aacatgataa ggaagctgaa
    16201 ataatatcta aagctggtaa attaggttct gttacaattg ctactaatat ggcaggtaga
    16261 ggaactgata tatcacttgg agctggagat aaagaagaag aagaaaaggt gaaagagtta
    16321 ggtggcttgt atgttatagg tactgaaaga catgagacaa gaagaattga taatcagctt
    16381 agagggcgtt cgggaagaca aggtgaccct ggaacttcta gattctttgt aagtttagag
    16441 gatgaagtaa taaaattgta tggtggaaaa agtatagaaa aaatattaaa gagaatgaat
    16501 gctaatgaag atgacgcgtt agaaagtaag gctttgaata gagcaataga aagagcacag
    16561 aaaggcattg aaggtaaaaa ttttgaacaa agaaagaatg ttcttaagta cgatgatact
    16621 attaatgagc aaagaaaagt aatatatgat gaaagaaata aggtattgga tggttctgat
    16681 attgaagaag agatacaaaa aatggttatg gatattatta cggaggctgc tgaaaagtac
    16741 ttaaaaaaat ttagagacta tcatggatat tttaagtatt tatataatac ttttatgcca
    16801 gctgatactt tattaattcc agacttagat aaaaaaaatg ttgaagaaat agtaaatcaa
    16861 acatatcaga tttcaaagcg tgtttatgat cttaagaaga tgatgattgg aattgaagaa
    16921 ttggctcaat tagagaaacg agtattactt caagttgtgg atacttattg ggttgaccat
    16981 atagatgcaa tggatcaact aagacaatat ataggtctta aatcttatgc acaaaaagat
    17041 ccatttaaag agtatgctat agaaggttat gaaatgtttg aagctttaaa taggaatata
    17101 agaataacaa cagtgcaata tctatataaa tttaactaaa aaagtgaccc attgggtcac
    17161 tttttatata ttaagattaa agtgaaattt attttacagt acacactcat atagataaat
    17221 atttataata atgaaaaaga ggctgaacgt cagcctcttt tcctgtgtga gaaatacgga
    17281 aaaaagagat gcttgaaatt tctttcttcc atttaatatt atactataaa gagaataaaa
    17341 agtccatgag ttatctttat gattaaaaaa aa
//
LOCUS       DBGJFU010000083        17296 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.083, whole genome shotgun sequence.
ACCESSION   DBGJFU010000083 DBGJFU010000000
VERSION     DBGJFU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.083"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(210..734)
                     /locus_tag="ACOIAS_002540"
     CDS             complement(210..734)
                     /locus_tag="ACOIAS_002540"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2702244.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(799..1551)
                     /locus_tag="ACOIAS_002541"
     CDS             complement(799..1551)
                     /locus_tag="ACOIAS_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGZ2702245.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1582..2880)
                     /locus_tag="ACOIAS_002542"
     CDS             complement(1582..2880)
                     /locus_tag="ACOIAS_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HGZ2702246.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2897..5161)
                     /locus_tag="ACOIAS_002543"
     CDS             complement(2897..5161)
                     /locus_tag="ACOIAS_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HGZ2702247.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5416..6234)
                     /locus_tag="ACOIAS_002544"
     CDS             complement(5416..6234)
                     /locus_tag="ACOIAS_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HGZ2702248.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6227..6925)
                     /gene="cbiQ"
                     /locus_tag="ACOIAS_002545"
     CDS             complement(6227..6925)
                     /gene="cbiQ"
                     /locus_tag="ACOIAS_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HGZ2702249.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6928..7206)
                     /locus_tag="ACOIAS_002546"
     CDS             complement(6928..7206)
                     /locus_tag="ACOIAS_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2702250.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7196..7948)
                     /locus_tag="ACOIAS_002547"
     CDS             complement(7196..7948)
                     /locus_tag="ACOIAS_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HGZ2702251.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8130..8322)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8454..10226)
                     /locus_tag="ACOIAS_002548"
     CDS             complement(8454..10226)
                     /locus_tag="ACOIAS_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HGZ2702252.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10240..11169)
                     /locus_tag="ACOIAS_002549"
     CDS             complement(10240..11169)
                     /locus_tag="ACOIAS_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2702253.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11398..11586
                     /locus_tag="ACOIAS_002550"
     CDS             11398..11586
                     /locus_tag="ACOIAS_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702254.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11579..12280
                     /locus_tag="ACOIAS_002551"
     CDS             11579..12280
                     /locus_tag="ACOIAS_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702255.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12282..13205
                     /locus_tag="ACOIAS_002552"
     CDS             12282..13205
                     /locus_tag="ACOIAS_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2702256.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13362..14285
                     /locus_tag="ACOIAS_002553"
     CDS             13362..14285
                     /locus_tag="ACOIAS_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702257.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14278..15015
                     /locus_tag="ACOIAS_002554"
     CDS             14278..15015
                     /locus_tag="ACOIAS_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702258.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            15026..15799
                     /locus_tag="ACOIAS_002555"
     CDS             15026..15799
                     /locus_tag="ACOIAS_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702259.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16340..16948)
                     /locus_tag="ACOIAS_002556"
     CDS             complement(16340..16948)
                     /locus_tag="ACOIAS_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGZ2702260.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17126..17242)
                     /gene="rrf"
                     /locus_tag="ACOIAS_002557"
     rRNA            complement(17126..17242)
                     /gene="rrf"
                     /locus_tag="ACOIAS_002557"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5583 a         2999 c         2178 g         6536 t
ORIGIN      
        1 aaatatataa acaatcataa taaaattttt ttaataaaac tttatttttt agttaaaatc
       61 atacaatata tagatttcca caatacaaat aaattaaaaa gtgaaatgta tttgtctaaa
      121 aacataataa catttcactt tatcctcatt tttaagtaat atatattttt aatttttatt
      181 tataactaaa tctagttttt atataaattt tagttttttt ctacatctga gtaatctatt
      241 ccaaaagttt ctacagtaac agatttcata atttgtggag tttgtggctt atcagctgaa
      301 tctgttctaa ctgatgctat ttcatcaact gtatccatac cttctatcac tctaccaaaa
      361 cctgcatact gaccatctaa gtgtggtgag tttttatgca ttatgaaaaa ttgagaacca
      421 gctgaattag gtgccatagt tctagccatt gatatgactc ctctttcatg ttttaaattg
      481 ttagtaaatc cattaccact aaattcacct tttattgagt gacctggacc acccattcct
      541 gtaccatttg ggcatcctcc ttgtatcata aatcctggta ttactctatg aaatattatt
      601 ccattgtaat atccttcatt tactaatgat acaaagtttt taactgtatt tggagctata
      661 tttggatata actcaatttt gatttctttt ccattttcca tttctatagt tactatagga
      721 tttttatttt ccataattta ttaccttcct tttcattagt taaattacat aattacattt
      781 attatattat actctaaatt aaagtctatc aagtatattt tttcctattt catatccttc
      841 ttgataaata gttttacaaa actcatcatc aaagttaagc gtgtctttag gagcaaattg
      901 agtattaggt ctaactacta ttattttatc ttcatcatat acttttttaa tatcttctgg
      961 caactcataa ttatattttg tggatattaa aataatttta tccacattat ccccaattaa
     1021 tggttcaatt aatgcactcc taattaaacc tccatctaat ttgaaaccat cataaattcc
     1081 ttcttgcaca tctttcacaa attgttcttt aaatccaaga gttgcgtttg gatttgatgg
     1141 taacaaccca ctgtatgaaa tgctgataat ggcttcatta cgaggcattt ttgatacatc
     1201 aacaatcttt tctgatacta ctttattatc aacttcaaca taattaacat aaaagttcat
     1261 ttcttctata ttactatcat ctagttccct taggttgtct atagcaggag aattatccac
     1321 agtattattt actattttta catttccatt ctggatatct tttatatttg tccacatttt
     1381 ctccaagtta tcaacattcc ctgtgtacaa ataatatcca tttattgctc ctattgatgt
     1441 ccctgaaatc gaataaaatt tatttattcc tccatcatac aaggctttaa ctactcctgc
     1501 ttgataagcc ccttttgctc ctccaccttc taaacataat cctaatttca tagtctcatc
     1561 tccaaattgt ttatcttata tttatatttc aaaaaactta ctaaatgttt tatatatact
     1621 atagtggtcc tcaacatttc ctaattctct tatagaatgc attgctaaaa ttggacttcc
     1681 cacatccaca gatggtatat ctatatgagt agaagatatt ggacctatag tacttcctcc
     1741 tctttcatct gacctattaa caaattcttg atactcaaca ttagcttctc tacatatatt
     1801 tttataaata gctatagaat atccatcact agtatacgcc tgactagcat ttatctttat
     1861 aaccggacct tttcccatta ttggtttatt agttgggtca tgcttttctg gaatgtttgg
     1921 atgaatagca tgagctaaat ctgatgatat cataaaagat gaataaatag actcaaagaa
     1981 tccctctcta tccttaccca ttgatataca aattctctcc aatatattta acaacatgtt
     2041 tgaatctgca ccttgtttag tagagctacc aacttcttca ttgtcaaata cagccattac
     2101 atttacacca tgttgacctt tagtattaac aagagcattc aaactagcat gtaccattgc
     2161 aagattatct tgtctcccaa tagatataaa ttcatcatta gcccctatta aacttccttt
     2221 ttcaaattca tataaaaata aatcaaaatc tataatatcg tccatattta catctaagtt
     2281 agatgctatt tgctttataa gaaaattatc tttttctaat gtatcattta acatcccaac
     2341 taaaggtaac atatcttttt gcttattata tgaatgacca tcatttatac ttctatttaa
     2401 atgtattgct agatttggta ttatacaaat tggcttttta aagtctataa gcctttcatt
     2461 tggttttaat atactatcac cttttaaaac tactctacca gctatcccaa gaggtctatc
     2521 caaccatgta cttaatattg ctcctccata gcattctgta tttaacttca aataagattt
     2581 ttcagcttct atttctgcat ttggtttaat actaaatgta ggagaatctg aatgagagcc
     2641 tattattcta aatccatctc tttctatatc ttctgaattt actacaaaag ctactatagc
     2701 tgataaattc ttagtaacat aatactttcc accaactctt agattccatc tttgattcaa
     2761 aactaattct tcaaatccat ttttatctaa gatttcctta gtactcttga ctgcatgaaa
     2821 agacgacgga ctatcatata tgaagtctat taagttttta gcaaagttta tactatccat
     2881 tcctcattta cctccattat aaaatattta gtgttgtata ttctttattt atttttttaa
     2941 ttccctgttg gtcaaaagct atagtaacat ctgtaccaat tatagaaacc actgtcccaa
     3001 ccccaaattt aggatgatgt accttagaac caagttttat atcatctgga ttagtttctt
     3061 tattagaatc ttttattgta gcattaactt tatctgcaac tttagatttg cttatagtac
     3121 tcatatactt tttagtatat ttatctaaca tattataact tgctttagaa tagcttagtt
     3181 ctttttttac tttgtacaat ctttctatac attcttctgg cagttcttcc ataaatcttg
     3241 aagctattgc aacatttgtt ttaccatata atgttctctt ctctgtcaat gacatatata
     3301 gctcttcttt agctctagtt ataccaacat aacaaagtct tctctcttct tctatttgtg
     3361 aatcactcat agattttata gctcttgata taggaaataa cccttcttcc atacctgtta
     3421 aaaacacaac agggaactct aaacctttag atgtatgaat tgtcataagc gaaaccctat
     3481 cattgtcttc ttcatcattt gattcagatg taagtgctat acttgttagg aatgtctcta
     3541 agtctttatt ttcataagta tcatcattac tactttcaaa ctccaaagct atagaaataa
     3601 attctctcaa attatcaatt ctgtcttgag cttcttcacc tttgcctgtt aaatcttttt
     3661 catttttatt tcttatctct acaagctcgt ccatatatcc tgtagtgtcc aagacttttt
     3721 ctataagttt gctaacagga taaacttctt ttatagttct aagagtccct ataatatcaa
     3781 caaatccact tatacttgcc cttgccttag tagatatatc tgaatttgtt tctatatcaa
     3841 ttaatactga gtatatactt tcctgtttta agttagctct atcttcaatt ttttctatgg
     3901 ttctaagccc tatacctctt ctaggaacat ttataatcct ttttatagat atatcatctt
     3961 gtggattttg aataactctt aaatacgcca acaaatcctt tatttctttt ctctcataga
     4021 attttgtacc accatatata ttgtatggaa tttgacttct atttaaagcg tcctctacag
     4081 gtcttgcctg tgcatttgct ctataaagta ctgcaaaatc cttataaggt ctattttctt
     4141 ttctagctat ttgtgcaatt gaatcagcaa tgaaatcagc ctcttctatt tcactaccag
     4201 ttaattgtat ctttataagt tctccttctt ttttttcact ccaaagcttt tttctttttc
     4261 tttctatatt attagatata actttattag ctgcatctaa tatcacttgt gtagacctat
     4321 aattttgttc caactttaca acatgtacat tgtcataatc tttctcaaat tcaagtatgt
     4381 ttcttatatc tgcacctctc catccataaa tactttggtc atcatctcca acaacacaaa
     4441 tattttgatg ttctcttgct aataatttta tcaattcata ttgagcttta cttgtatctt
     4501 ggtactcatc taccattatg tatctaaatc tacttctgta atatgctaac acttcatcat
     4561 tagccttgaa aagttctaca gttttaagta ttaaatcatc aaagtcaagt gcactatttc
     4621 tttttaaacg gtcttgatat agcgcataaa tatctgcaat cttaaccatt ctattatctg
     4681 ccatattgat atctttaaac tgctttggag tataaagttt atcttttgct cctgaaattg
     4741 cacttataac tgcttttggt tcaaacactt tatcacttaa atttaattct tttagacagt
     4801 ctttaaccaa tgtaacttgg tctgctgaat cataaattac aaaacttcta ttgtaaccaa
     4861 tcttatttat atcttttctt agtatacgaa cacagcaaga atggaatgta cttatccaca
     4921 tatccttagt ttcaggacct atattttgtt caactctctc tctcatttca tttgcagctt
     4981 tgtttgtaaa tgttatagct aatatatttg gtgcttgtac acctttatct tctataagat
     5041 atgctattct tgttgttaat accttagttt tacctgaacc agcaccagcc agtataagta
     5101 ctggaccttc agtcttttct actgcttctc tttgagcagg atttaatgta tctaaattca
     5161 tatttctcct cctgttaaca gccataaata tattttaatc taaaatatga attacttcaa
     5221 attataatta tgtattatat taatagatta tttccaatat attacaatta aataacattt
     5281 ttccatataa tttatttcat tgtttaataa aaaaaataac cttatgattt ggggaatcat
     5341 aaggcacctt taaggggtct aggttaaggg ttattatata taaatctatt tttttaaaat
     5401 aggtttaggg cttaattatt tatttgtatt aagttctctt tccttccaat acttatacaa
     5461 atcttcttct ttcttaaata agggtaaatt catatttcta tgtactttta ctaaccatgg
     5521 ggattctagg ccagcttgta taatattatt ttcatttata aacacatttt ctgcttttcc
     5581 ttcatcaatc aaaatcccct tatccaatac atatatgtaa tcacatattt catacattaa
     5641 attcatatca tgactggata ttactatttt tatattattt ttatttaatc cctttattat
     5701 atctacaata gacctagtac ttactgggtc taaaccagct gtaggttcat caagtaatac
     5761 aatttcattt tccattgcta taactgatgc tattgccact ctttttttct gtccatagct
     5821 aagaaaatgt actggcctgt ctataaaatc tattccatta acagctacta gagctttgtt
     5881 tattctctct ttaattgttt tttcatccat accaatgttt ctcattgcaa aggctatatc
     5941 atcatatact tttgaataaa aaatttgttt ttctgggtct tggaacacaa tacctacatt
     6001 ttttctaagg ttgtataaac ttctcttatc atattttaac ttttcttctt taaaaagaat
     6061 ttctccagat gtaggcttta atatacccat taaattcata aataatgttg attttcctga
     6121 accatttgaa cctattatac ctataacatt tccctttgaa aaatccatat taatgtttaa
     6181 tagtgcattg gtattttttt catattgata cgttaaatta tttattttaa acatcattat
     6241 cccctactat gtgaaattta ccatcataca attttatgtc taatgatata cacatatcat
     6301 cataccttgt cataagtctt ttgtacagca tacttccaag aatacctaag gatttatatg
     6361 aagtttttaa atttatatat ccaaatctta gctcttgtga cttgtatatt tctgaaaatt
     6421 cttctaaaaa tatgaatata aacctatata ttaacattga taactcgaca aaagtgtcag
     6481 gtaaatgaag ttttttaaaa aagaatatta attgattaaa aggtgttgta agtatacaaa
     6541 aatacacaca agtcaaacaa gacatcgctc taaataacac atgaatagac atgtcaatag
     6601 acgcctttga tattcctaca ttaaaactaa aaaattcaaa actatataat aaatctgctt
     6661 tattaaaaga tatatttaca agagttattc ctatactaag aaataaaaag tacattggta
     6721 ttctaagtaa ttttaaataa ttttttaaat ctattccagc cacacaaaca accaaaatac
     6781 tcattaaaat cataattcca cttaaaataa atatattttg tattaccata gaagcaatca
     6841 gaaaaattac tcctatggct acttttttgt ttggatttac tttactcaat ctatttgtat
     6901 atgcatattt atcaattata agcatgatta acttctcttt cctttttgaa gacctaatat
     6961 gtatcctatt acaccagccc ctaaagctgc ttgtgcagag aataataaac tttctatttc
     7021 tccacttggt ggctcatatg gactgctaaa ccatggtttg taatttggat ttatcttagt
     7081 aatttcactt tcagcttgac catctgctcc tccatattca gcacctgagt tgacaagtaa
     7141 tgggaatata ataagagcta ctactaataa aagtaataat atgttagttt ttgttttagc
     7201 actcattagc tttaactcct ttatcattat tttctagtat gcttttttct ccgttttctg
     7261 taattaaatt atatacaatt acagtaagta taccttctgc tattgctatt ggtatttgag
     7321 tcataaagaa aattcctaaa aatttaattg ctgaagccat taccccacct gatgggtctg
     7381 ggaaagccag tgctaattgt attgaagtta ttgtatatgt ggctaagtct cctattgctg
     7441 ctgcaagaaa tactggaata gcattatttc catctttttt ctttaaagcc ttaaatatta
     7501 ggaatgatac gattggccct attatcgcca ttgaaaaagc attagctcct aaagtagtta
     7561 ttcctccatg tgctaataac aatgcttgga atattaaaac tatagttcca agaacaaaca
     7621 ttacgcttgg tccaaacatt attgctccaa gacctacacc agtaggatgt gaacaacttc
     7681 ctgttactga aggtattttt agtgctgata atacaaacac aaatgctcca cataaagcta
     7741 ataatacttt ctttttaggg tcttgcttta ctattttacc tattgatttt aatccgaata
     7801 agaaaaatgg tataaatata actccccatg ctatagacca ttttactggt aagtaacctt
     7861 ccattatatg cattgcattt gatgtaactg gagttaaaat gattaacata agtgcagcta
     7921 taacccctaa tttaatattc tgtttcatct tagtatccct ctctcccttt ctaataatta
     7981 aaacataata atacatgtct gatttattta ataaaataaa aaagcctaac tcgatggtta
     8041 ggcttctaat tttccacaat ctaaatacaa acacataaaa caaacatatc tgtataatta
     8101 ttataagaaa ctttctccat ccccgaagaa attactttaa tattttaggc aggtctcctg
     8161 acttagcttc atcctactcc tcttccttcc catgagatat aaaaaatatc atcatagtgg
     8221 ctaacaaaag tttcgtctgc atcacagtag cgggggctgt aaaggaattt aacctttttc
     8281 cctattaatc ttgcttaaag aacctataat attatatatt taatttttaa attcttctta
     8341 aattattttc tatataaatc attgtatacc ttttctgtga aaatttctac attttttata
     8401 aattttattc atttttatat ttttatgtat ataaattctt ttttgtaagt ttattaattt
     8461 ctaattatct tctttaaata cattgaaact acatcagaaa atctagttat atctcttgaa
     8521 tatataaaat catttccata tattaatctt tcagcttcaa gttctgattc ttttcctgta
     8581 aatactccta atactaatat attttgcttt ctagcttttc tcacttcatt ggctgtatct
     8641 ctcaaaccaa ctatacccct ataagccttt tctcctctta atgttctctc actacttttt
     8701 cctatcttta catcatttgg tcttccatca ctgagaacta tcaggatttt attttcttca
     8761 tctctattta ttagaccact acatatagat tttattgcca aaccatctct attattacca
     8821 gcacaaaaat attcaaatat attttcacta ttttttatac tatcatcata atctttaaat
     8881 ctttttaata ttgtatagtc taaaaaacta gaaaatccca tcactctatt tggtatgcca
     8941 acttctgtta gagcatttgc aattatgtaa gcttgaattg ccacatttcc ctgatttcta
     9001 ctttgagagc cacttgcatc caataatata tcaataacgt atttcccttt ttcattttct
     9061 atatctttat aaaatatttt attattatta cttctaccaa ctctccaagc tctattggct
     9121 aatatagtac cacaatcaga atatactcta cttttagcat tttcttctat taaaattctt
     9181 gatagacttt cttttaatct ctttatttgc tgtctataca ttctagcatg ttctctaaat
     9241 tttcctaaat tattttcttt ttgtcttgtt acatatttta gttgaaatac attattacac
     9301 tcacttctaa gaactccatc tgtaaagtgt attctacaac cttcatgaac atttctacaa
     9361 ttttgtgtct caattttttt aatttcacct ttactcaaat aagtttttcc ataataatgt
     9421 tctatttttg aatatatctt atttgcagtg tcttcatcaa catatataac tcggtctgct
     9481 gaattgctac atttagaatc atcaaaatcc cctacacctt ctacaagcat ggatgaagat
     9541 attgaatcta tatcactttc aataatattt tcttcttcat cactataaag ttcttcatac
     9601 atgaaatcag caaatgtatc aaaatttact tctacacttt ctacattatt ttcatcaata
     9661 agactatttt cttctccatt taatatgtat tcaaaataag ttaaatatat aacatctaat
     9721 gcttttaaaa tctccataat atcttgattg gtatctatat ttcttatatc attaactatt
     9781 cttctagtca tactatctaa tgctgggtgc ttgtccatac tataaagtac aaatgtgtat
     9841 ttaattttat ccataatatt atcattgtgt attttggtaa aacgtgaaac aatatcttta
     9901 taagcatttc ttcttatgtc ttctatccct ggcctatctt taatgacctt ttcttcaact
     9961 attgtattta atataacttg aattagaccc agtataatat ctttgttgta tccttttctt
    10021 attctatttg taagatattt tttaactata tcccaatcta catactttct tctagcacct
    10081 gcaattattg caaaatatag acctatttct tttgaagagt aatctttttc tgatatatct
    10141 atactttcat tgtaatcacc agctattgtc catgataagt tattagctct attttcaaat
    10201 tcataatcat catatatcca tttcataatc tcacctatat taaatatgac ttattggaaa
    10261 cacatctata ctctcccatt tattaggtat tctagtttta attacatctc caaccatttc
    10321 tttctcatat acatcaaaag tctttcctgt aagtcccata ttaattgcaa gagttggttt
    10381 taatcctctt cttatagtct taagagatgc cataagacca cgtaaatcaa tagctttact
    10441 agatatctct ccattttgag acttcaattg taaatctaaa aatattcctg caaagtgtat
    10501 tagtttttct tcatcagcat ctgaaaattc attttttaaa atcatcatta acttatcttc
    10561 ttctatagga ggaatatcta ttaccataaa tctagatacc agtgcttcat taagttcttt
    10621 tgttcctgca tattcataat tcatagtccc aataaatctt gttgctggat gtagattgac
    10681 tctctcatac ccaggtacat caattattct tctatagtct aaagctgaat gtaccacaac
    10741 tatagcatca ttttttgcca tattaatttc atcaaacacc ccaaaacctc cattaatagc
    10801 acactcataa actgagcctc ttctaagttt aacttcattg tctataaatg tatctgtacc
    10861 tataagtgtt gaactgtctg tatttatatg aaatgatgta ttccactgag gtctattaaa
    10921 tatttcccct agattatcag caagtaagtt ttttcccgtt gcctttggtc ccgataataa
    10981 tatattttct tcttctaaaa tagctgctat acacatagac aagatatctt taccataaaa
    11041 ataagtttta gactttgtta ctctgtattc taaatctttt tctaatccat aataatttct
    11101 aaaatacagt acatcttcaa taagtttttc atctacccct tgcaccctta gattatctat
    11161 tatatccata tttttacccc ttttatataa atagtatttg tttcatttct tatcaccatt
    11221 ttagcataaa aggatataag ctaaaattga ttttttaatc tcccccattt taagtaaaac
    11281 catgcaccat tttatcacta caaaattaag agtactactt actgatattt cttgatttta
    11341 ttcgtctata cgtatataat atatactgat aaattatgag gaaaggatga ttgaaatatg
    11401 gaatatatgt cttgcccaga agtagcaaaa aaatggggta tttctgaaag aagagtacaa
    11461 aaactatgtg aagaaagtcg cataccagga gtttcaaaac tcgggtatat gtggctgata
    11521 ccaaagaacg cagaaaagcc tgttgatggt agaacaaaaa gaagaaagga agtacaatat
    11581 gaatagcgtt ttgattatag atgatgacaa agaactttgt gctcttatga aaaaatgtgt
    11641 tgagcaagaa aatttatctg ctattactgc acatggtggt gcagaaggtc tacatttagt
    11701 cgataaaaat agacacacac tctctcttgt aattttggac ataatgatgc cagatataga
    11761 cggttttcaa gttctgaaaa aaattagaga aacaagtaat attccagtaa ttatgcttac
    11821 tgctaaaaat gacgaggatg ataaagtttc cggactacgt ttaggtgctg acgattacct
    11881 aacaaaaccg ttcagcataa acgaacttat ggctcgtgtc aattctctta ttcgtcggta
    11941 tactatgcta aaccctgctt ttataacaga cacagatttc ataataataa atggcatgat
    12001 tattgacaag acaaaccgaa ttgtttctgt caataatatt cctgttcaac ttacaagcaa
    12061 agaatttgat ttacttgctt ttctggcttc taataaggga cgggtgttta ccaaaaaaca
    12121 gatttataca caagtatggg aagaagaata tgcttttgac gatagtaaca tcatgtcttt
    12181 catcagtaaa ttgagaaaaa agattgaacc agaccccaac catccatttt atattttgac
    12241 agttcgtggt gtcggttatc gttttaataa ggaggcttaa catgaataca agtgtctatc
    12301 tgcttttgat attggctctt agcctgatta ttatagcgta ccttgtttct gtcctgctac
    12361 gtgtccgaat acaacttaca cttataaaag atgctttaga ggatataaaa aatggaaacc
    12421 ttaatcgacg tattttaaca gatgaaaacg acattacaaa acaaatttgc tatgatatta
    12481 acgaaatagc aatcaatagc caatttcaac ttattcagca aaaacaatca gaacaagcct
    12541 acaaacagct aatgacaagt ctctcccatg atgtaaaaac tccacttgct tctttggtcg
    12601 gttatttgga agcagtagaa agtaaaattg ttgtaggaga ggaaaaagac gagtatatcc
    12661 atgttgcgtc taataaggct cattacttaa agaattttgt agaaaatcta tttgaatggg
    12721 taaaattgga ttctaaggaa caagtttttc attttgatat ttttgacttg aatgaattat
    12781 cccgtaatat tatatctgat tggatacctg ttttagaaag cagtcatttt gaatatgaat
    12841 ttgatatacc agaaatagag tacttcttac gtatagacgc aaatgcatat acccgtatca
    12901 ttaataacct attacaaaat attattaccc atagtagtgg aaacaaaatg acattgagaa
    12961 tttttgaaaa caaagagcaa gctcaaatta taataacaga taatgggaaa ggtatatctt
    13021 cagacaatct cccacatatt tttgaaaggt tgtaccaatg cgaccattca cgagcttcaa
    13081 agggaaatgg tttaggcttg gcgattgcaa aggaacttat taacgctcat aagggaaata
    13141 ttaccgccaa cagcactccc ggtatgggaa cagaatttac aatattgctt cctaaagccc
    13201 tgtaaaagta cgaggctttc ttactctaaa acaagataaa agcaagatta tggcaaggtt
    13261 ttggcaaggt tcatgtttta taattctaat tgtagttgat tttgacgttg tatcaactat
    13321 cttttgtaga aacaaattta ttaaaagtga ggaaattata tatgaatgat tttgtgattg
    13381 aaacaaagca acttacaaaa atttatggga atcaaacagt agttgacaaa gtcaatctcc
    13441 atgtaaaaaa aggccgaata tatggtctgt taggacgcaa cggagctggg aaaaccacaa
    13501 ttatgaaaat gattttagga ttgacttcaa tcacgtatgg cgaggtagat gtatttggag
    13561 agaatattaa gggacgtgaa aaacaagtat atccccgtat cggtgcaatt atcgaaacgc
    13621 caggatttta tcctaatctg acaggaacgg agaacttgga gatttttgca aaactgcgtg
    13681 gtacagccgc ccctaattct gtaaaaagag cattggaaat tgtaggctta ccttataaaa
    13741 ataaaaagct gtttagtaaa tattcacttg gcatgaaaca gcgacttggt attgccaacg
    13801 ctatcctgca tgacccagaa ctgttagttt tagatgagcc aaccaatggt cttgacccta
    13861 ttggtattgc ggagatgaga aagtttatta gggacttgag cgttgagcat ggaaagacaa
    13921 ttcttatttc cagtcatatt ctttcagaaa tctcattgtt ggctgatgat attggaatta
    13981 tcgaccacgg tgttcttttg gaagaaagta gtatgagtga actagaaaag aaaaacagta
    14041 aatatatcct gctacaagtt tcagatattc cgaagacatc acttatttta gagcgtcaat
    14101 ttaacacgaa ggattattcc gttcaagatg actacacgtt acacctttat aacactaatc
    14161 tggatatggg tgaaatcaat aaagcacttg taatgcagaa tgtaactgtt attagttctc
    14221 aactttgtaa tgatacttta gaggactact ttaaaaaaat cacaggagga gagggcattg
    14281 cttaaactta ttcaaacaga atttcttaaa ctgcgtcgta ggaaacttat ttggctgatg
    14341 ttgttgtctg ctctcattat gccgtttttc gcactactat acttcaatta ttttggtaaa
    14401 acaggtgttg agccaataaa gttttataaa tggtctgcat ttggttatac actttggatt
    14461 attttgcctg ttgttttggg tatattttgc accatgctta tacacgaaga aaaccagtat
    14521 gatatgctca aacagctttg gattgtacct attagtaaaa tgggatactt ttttagtaaa
    14581 tttttcgttg ttttgattta ctctatttgt tttatgctga ttacagccgt tgcttctgtg
    14641 ttatttagtg tactttctgg atatgtcgta tttagctggg caagcatttt atatttgttg
    14701 aaaaaatgtt tagaaattgg tgtaatcact gcttttgtaa tgttgccgat tttggcaatc
    14761 gctacatctc aaaaaggata cattctccca atttgtatca cattggtata tgccttttcc
    14821 ggttttgtta taatgacaat aaatatgtat ttacacccat tatccagtat gggagttatt
    14881 atcatgagaa ataaagacat tccaggtctt atattttcac aggaaattaa tgtcccatta
    14941 gcatttcttt gtatttgtat ttggagtatt gtatcagtat tgtttgcaaa tattgcacta
    15001 ggaagaagaa agtgaggtgt agataatgtg gaaattactt tgggctgaat gtcaaaaaat
    15061 tcgtcgttca aaaattgttt ggattacagt ttttgcatcc attatgatag cagtcatagt
    15121 ttttgcaggg gggcaggatg tatataatga cccagattta cattatggct taaagagcgt
    15181 ttatgacggt tcacggtata tagacaatgc aggttggtat atggacgcag tacaaccttg
    15241 gtcaactttt tttgttcttc ctgctgtcat tgccctatta ggaagctata ttatttgtcg
    15301 tgaagaagag gaggacacta ttaaatccct gcgtcttatt cccataaatg aaacaaattt
    15361 aaccatagca aaaatgatta ttacttttat attcagtata cttctttatt tgttactctt
    15421 tactataaca tttttgactg aatccgtttt acatttttcc gatttatcga caaaattggt
    15481 tttaagttgc atgaaagagt attttcttga tggcataggt gtattctttg caatttctcc
    15541 cattattgcc ttggtatcgc gcatgaaaaa aggatattgg cttgcattag tattcacaga
    15601 agtttattct attgctggac tatttgcggg tatgtcaaat gtcttacaaa ccttttttcc
    15661 tattgcggca atatttaata tttctggata ctatataact actggagaaa aaattatggg
    15721 gagtatcatg agtttattac tttgtgcctg cctttctgca tttatactta aagggctaaa
    15781 acacaatgaa aaaaattgaa ataggtatat caaaatgaaa aagataaaaa aactgattat
    15841 tctaattgta ttcttgtttc tttttggcat gttacactgt tatactgaat ggaaattcaa
    15901 caataaccca cctaaagggg taatagaaag aataaatgag tggactgata agattggact
    15961 ggattagtaa cttatgttaa ttatagtgaa tgtaatatta gcaagttatt gtttcgtatt
    16021 ttaatactat aactttaaat atttggtttt aatcttccaa acgaatataa aaaaactgtc
    16081 acagaggcaa catatttttg aatatttata aagtggtagt tttgattttc aaagctaccg
    16141 ccttttcttc tatataagca aacttatcct caacaaagtg taaaacatgg tgcattttaa
    16201 gaaaaaagta ccacagacac aagcaataaa agaaacgccg agaacctaca aaaacgtagt
    16261 gcttatgcag atttacgacg ccttctattt tctcaaccgt cttaatgacc ttgtacaaaa
    16321 gaaatttttc taatattttt tataatctta tcttgtgatg ttttttatgt ataaatgact
    16381 taataacaaa tccaataaaa attatggata ttggtataat tagaacgagg aatttataat
    16441 cattataatg tgacattatt cttacccaac tctcagaaaa aatatatcct atactgatta
    16501 atgaagtatt ccatatggtt attcccacga tagatgatat tacaaaagtt ataatatttt
    16561 gacctgctat acctgctata aaagatatgt atgttctaca taaagggatt aatctacata
    16621 cacaaacaga aatagaagag tatttattaa aataatcttg agatgcaaat attcctttct
    16681 ttgcctttgg acatatctct attattttat ttatgataga tttacctccc caatttccca
    16741 ctgtataaca aaatatcgaa ccaagtatac ctgcaattat acttaaaatt aaaattgtca
    16801 gaaatccaaa atgatttctt gcagcaattg ctccacataa aggtaaaaca acttcgctag
    16861 gtaatggaaa acaagcatac tcaatcataa ttaatacaaa tacagagatt aatccatatt
    16921 gtaaaataaa actattgatt acagacactt acttatctat ctcctttctt atttaaaggt
    16981 tttaagggta catgttctaa ggattctcac cctatattta aagaatcaat ctctttaaac
    17041 tattatatgt atttacacaa agcatttata acatacttta aaaatagtac cttaaaataa
    17101 gaaacttaat aaataaaaaa agattacgtg gctacgtcct actctcccag gcagtttccc
    17161 accaagtacc atcagcgcta gagagcttaa cttctgtgtt cggaatggga acaggtgtat
    17221 cctctctgct attgtaacca cataatcttt atgcttaata tttccattaa gtttttaata
    17281 agtttataga acctag
//
LOCUS       DBGJFU010000084        16794 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.084, whole genome shotgun sequence.
ACCESSION   DBGJFU010000084 DBGJFU010000000
VERSION     DBGJFU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.084"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            167..562
                     /locus_tag="ACOIAS_002558"
     CDS             167..562
                     /locus_tag="ACOIAS_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HGZ2702261.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            605..2704
                     /locus_tag="ACOIAS_002559"
     CDS             605..2704
                     /locus_tag="ACOIAS_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGZ2702262.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYNGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(2929..8139)
                     /locus_tag="ACOIAS_002560"
     CDS             complement(2929..8139)
                     /locus_tag="ACOIAS_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGZ2702263.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKEIM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWIISKREDSGSA
                     GAEGTAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDFKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8154..10649)
                     /locus_tag="ACOIAS_002561"
     CDS             complement(8154..10649)
                     /locus_tag="ACOIAS_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2702264.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(11286..11573)
                     /locus_tag="ACOIAS_002562"
     CDS             complement(11286..11573)
                     /locus_tag="ACOIAS_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702265.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(11593..11868)
                     /locus_tag="ACOIAS_002563"
     CDS             complement(11593..11868)
                     /locus_tag="ACOIAS_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702266.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(12505..12684)
                     /locus_tag="ACOIAS_002564"
     CDS             complement(12505..12684)
                     /locus_tag="ACOIAS_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HGZ2702267.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(12969..14444)
                     /locus_tag="ACOIAS_002565"
     CDS             complement(12969..14444)
                     /locus_tag="ACOIAS_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2702268.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
     gene            15513..16691
                     /locus_tag="ACOIAS_002566"
     CDS             15513..16691
                     /locus_tag="ACOIAS_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2702269.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFRNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT         5647 a         2868 c         1863 g         6416 t
ORIGIN      
        1 aactatataa actacatatg taaaattcat gtaaatttac taatataaat caaataaaat
       61 tactaaactc tctaaactaa ttttgtaaaa catactctat ttttaacatt tgttacctta
      121 tacataagta caaacaaaat ctaactaaat gaaaggataa tgtaaaatga atattttaaa
      181 aatgaaaaaa acagggatat ttttaggagg agtacttttt ggtactgtag gtgttaagat
      241 actttcaagt gattgtgtta aaaaaacttg cactcgtgca acagcaggag cattgaaagt
      301 taaagattct gttcttgaaa caactacgaa aattcaagaa aatgtagaag atattttagc
      361 tgaagcaaaa gaaatcaacg agcaagaaga cttatctaca tctaaggtaa acgttgtaca
      421 agatactctt aaatgcgaac aagaagttct taaaactgaa actataaaaa ctgtagaaga
      481 tactaattct aatagcatta aatgtgaaga aatcaaagat aacgtaattg aagaaaaaaa
      541 tagtacttgt acaacagaat agatttaatg attattatga ttaataatat caggaggaag
      601 taaattgaat ttttatataa aacacgaaat aagaggtcgt atacgctttg acttacaact
      661 tggcaaatta aacaacaagc agtccgatac tctcttatat tatttaactt ctataaatgg
      721 tattaccaaa gcaaaagttt acgatagaac tggtgatgca gtcatttatt acaatggtga
      781 aagagaatac ataataagag aaatcactaa attcagtttt aacaatagca aaatagaaaa
      841 cctagttcct gaacatacca gcagggaatt aaacaattat tatagagaca aattagcaaa
      901 taaactgcta gtcaaaagta tctccaaaat attttttcca aagtccttaa gatttttttt
      961 aattggactt cattctataa aatattttaa aaaaggtctc tcttccctgc taaataaaaa
     1021 aatagaagtt tctgttttag atgccactgc tataggtatt tctattttta gaaaagatat
     1081 caatacggct ggttcagtta tgtttcttct tggtatcggt gaactattag aagaatggac
     1141 tagaaaaaaa tctatagacg acttggctca aagtatgtca ctaaacattg aaaaagtatg
     1201 gttaagaaaa ggtgacactg aaatcctaat tccaatttca gagataaagg aaggagattt
     1261 agtaagtgta actatgggaa atatgattcc attggatgga gttatagtat caggagaagc
     1321 aatggtcaat caggcatcac ttacaggtga atcccttgca gttaacaaaa aagaaggatc
     1381 ttacatatat gctggaactg taatagagca aggcaatatt gttatgtgcg tcaaggaaaa
     1441 agctggtact actagattcc agaaaatagt aactatgatt gaggaatctg aaaaactaaa
     1501 atcttctgta gagagtaaat ttgaacatct agcagataca ttagttccat acagcttttt
     1561 aggaagtata ttaacctatg caattactag aaaccctata aaatcactct ctatattaat
     1621 ggtagatttc tcttgtgcat taaaactctc tatccctatt tcagtactat cagccatgag
     1681 agaatgtaac aataacaaca ttactgtaaa aggtggaaaa tttcttgaag gtgttgcatg
     1741 tgcagatact attgtatttg ataagacagg cacattaaca aaagctcaac caactgtttc
     1801 agatataatt acattccaaa attataataa ggaggatatg ttaagacttg cagcttgctt
     1861 agaagaacac ttcccacact ccatagcaaa tgcagtagtt tatgaagctg aaaaacaagg
     1921 gcttagtcac aaagaaatgc atacagaagt agaatatatt attgctcatg gcatttcaac
     1981 aaaagttgat gataaaaagg ttgtaatagg tagcaaacat tttgtatttg aagatgagaa
     2041 gtgtactatc ccagacggtg aggaggataa gtacaataat ctatcagatg agtattcaca
     2101 cctatttatg gcaatatcag gtaaattatc agcagttatt tgcataaatg accctttacg
     2161 aaaagaagca aaatatgtaa tttcaaattt aagagaatgc ggaataaaaa agattgtgat
     2221 gatgactgga gatagcgaaa aaacagctaa atctatagca agtaaagttg gtgttgatga
     2281 atattattca gaagtcttac cagaagacaa agccaatttt gttaaaagtg aaaaactcaa
     2341 aggtcgcaaa gtaattatga ttggtgatgg aatcaatgat tctcctgcta tttcagaatc
     2401 agatgtcgga attgcaatga gtgaaggtgc tgaaatagca agagagattt cagatattac
     2461 catatcagca gataatttga ataatttaat cgttttaaag cagattagta ataagcttat
     2521 gaaaagagtt gatttaagtt ctaagtttat tatcggcttt aatttaggtt tgattttact
     2581 tggtgtaggt ggatttgtta gaccaagtac atctgccttt ttacacaacg catctactgt
     2641 cggaataagt ttaaacagta tgacaaattt attggaaaac accaatactt ataactacca
     2701 ttaatctcca ttacataaaa catatgatat aaaaaattta taattcatct ttttaaaaaa
     2761 atatgtctta aaattagctt ttttctaatc aaaattatat aaagcatact ttacaaattt
     2821 taaactcaac ttatttctat tttttacaaa aaaggagatg attcaaattt tcattttgaa
     2881 acatcccctt ttgtaaattt ctttaaaatt tatatattaa ttatattttc aaccaataat
     2941 tgtattttca accaatcttt cttcatagca ttttaaatca ttttgcttta gataattaaa
     3001 ttcagactct acataaatac cacagcctac caattcaatt gtcaaattag gtatattttc
     3061 ctttagtata ctagtctgct cataaataat tctttccaat aaatcaactt gtaatataga
     3121 aaaatgcttc ataatctccc catcactatc tatatctttc ttgatttcat accaactctt
     3181 tgacaaaatt cccttaaaat caatataccc tagactcttc aatatatcta tactatcgct
     3241 ccatgcatac ttactaggtt tatcaaagta tgactctagt ttcttcttct gctcatttgt
     3301 aagtgaatca taagaaagct tcttattaaa atgtgccttc aaagaaatct tagccataga
     3361 atatggactt ctaccagagt ttaatcctgc atttttgaac ttttcaattc ctttatcttc
     3421 tactgaagac catattgagt ttattgaatc tgaatcattg tatttaacat caagaccatg
     3481 tgtaatattt atatagtttt gtggaaatac atattgataa tagacatctt ttttatcatc
     3541 atatagagag aagtatccac tatcatataa atgaatatgc acctttactt ttaaactatc
     3601 ctctaaaata tttcgtctaa ggttcgggat atttgcatac atggttacat attttatggc
     3661 atcaaagttt ttatttaatg gaacttcaaa actaatttca tctcctgtca aaagcttttt
     3721 cacttcttct tcattacaat catatagaga atatgtattt tcaaatccca cacgtgattt
     3781 cccatctaaa ctgcctgtta atgctacctt caaaatatgg ttatctgttt tttgccattg
     3841 gtaagcatct tggtcaagaa actctctaat ctgtctattt tgctctttcg cctgatttat
     3901 aacctcgttt tcatcttctg taatccctac atggcatcta tttaagatat ttactatatt
     3961 gcttccaaat gggaatgtcc atagttgaaa agctgctgcc tcaacctgac acaagtatct
     4021 aacctgattt aattcaattt gatggataag ttttttctga tattcattca tctgattacc
     4081 actatctgac atttgataaa tcaattctcc cattgcaaga ttatcttttt tcttatcaat
     4141 cttatcagta atgataattt gagtttccat catatcctgt acttttttaa atattataga
     4201 aatctcctct gaattttcca tttttaagct tccaaacttt gtgcttaatt cagttaaaac
     4261 ttccttcatc tttaatttac ttatctcatt ttcaaaaata tcttgatttt taactgtact
     4321 atcgaatttt tctacaaatg gtataacaga ttctttcaaa gttttatctt taaattccac
     4381 catttgagta ttcaattcat ctaatttctt aatattattt tctttatctt taattgcctc
     4441 tttaactacc tctattttac tctcaaaact agattttaca ttaagacaat tattaacacc
     4501 actaatcgca gtatttataa gagaacaagc agaacttcca atactcaatg catttttaat
     4561 tttagatgtc ttttgctgtc tttcaatagc ttgtttactt ttctctagtg cagctaactt
     4621 atctttttgc tgtttattat tgtaatttgc ctccaacata cctatttctt ttttctggat
     4681 ttttgtgtaa atttccttat actcgctttc acttaacccc tcttttgctc tttgaatatc
     4741 gtcagctttg tctgcataat tatcatcatc tatagactta actataatct ccttaatttg
     4801 gtcaaattta gttcttcttt cttcatcaac acctgccaaa ttataagtca ttttatctaa
     4861 agcatttttt ctatcttcac ttagctgttc tccaagtaca aatctcatac catcagaatc
     4921 caatttaaaa tctgaaaaat taaccatcct gttagtccat ttgtattcct cttctttagc
     4981 tgaaagattt tccttcaaag attttgcact gtcaaatgcc cctagacaag cttgaccaac
     5041 accactaaat gcagtcagta aactcaatgc gctttgtatt ccagaaagaa ttaactgtgt
     5101 tttcatttgt tcctcaagtt ttgcctgttt cttttttagt tcctctatgt cttcttgttc
     5161 atcttctttc atctttataa tttcttgaat caacaggttc atttttgcat taacagaaag
     5221 cattttattc tcaagttcat tttttaaaac ctctatttgt tgatttccag attcaatttg
     5281 agttaggata ctttccttat atgtattgaa atatccttct tttaactcat ctttcttccc
     5341 ttgttccaat tctcttaagg tcttatccag ataatctgtc aaagaacgaa tatctgtaac
     5401 acgatttcct ccaatttcac tttcttcaat catctgcaca taaaatgctg tcttctgctc
     5461 aagtgcaaat aaaatcatac tccattcgct aaaattctga ttttttccta caaaagattc
     5521 tattctatca gactgcatca atttatttat aaggtattca tagatttcct ttgcttcttt
     5581 ataaaattct ttctttgtat tcatatcatc cagagaaagt actgctttat ttttcttctc
     5641 aaaactatag tacttctgaa gtatttctct atttaattgc atctctattt tctcaactgc
     5701 atgaaaaatt tcataaatat gtgaaagttc taaaatatca gcttctaaaa ttcctactgc
     5761 acacaacagt ctaagatttc tataaccttt aattccagaa aaaccaaatc tctcgaaaaa
     5821 ttcatagaag aatgtatcta ttgacaaaga atactcctgc tttaacaatt ccttttcttt
     5881 tagtgcatct ccagagtgac cggctgttcc ctctgctccg gctgaacctg aatcttcacg
     5941 tttgctaatt atccagtcct tatgttcttc ataaagagca tttaatttct ctccatcatc
     6001 tgctgcttgc ttctgattta aagcatgatt aaacacatat ctatcaaaat aatagtagtt
     6061 tctatcacta ttatccattg gaggagaggt acctatatat cttattgttt cttcatatat
     6121 acctctaatt ccaccaagac ctcctgcacc accagctcct ccagagcctc cttttcctgc
     6181 taaaccatga tttccatctt ttttacttat agcacttttt aataaaggag cttttccaag
     6241 gagataaacc tgtccagact tgcttccaag acctgcattt ccaccattac ctcctgtgcc
     6301 tccagttcca ccttgttttc ctttttctgc ttttaccaca tagtattttg tacctttaac
     6361 attagcaaat tcttttgact cttttttaga acctatttta gcatttgccc cagcttctcc
     6421 agttatccct gtatctcctt tttttccatc ttgtccattt ccaccacgtc caccatctac
     6481 gtaaagttta agttctcctc ttttttctat ttctttaaaa gataatatga tatcacctga
     6541 agtacctgca ttattgccat ttcttccatt cattccgtcg taaatagaac tttctccaac
     6601 caatttttct gcatccttac cagatacgtt gattgaataa gtcccttctg gtatatgtaa
     6661 tttttctgta agtagtacaa tattcttacc tgagaagact tctttctcca tattacagtc
     6721 aattttcaac tcacaatctc catacatata tatagatgac aaaatattaa gttgataacc
     6781 agatattatt ttagtcaaat ccgataaatt cactttaggt ataggaatat atatagaaag
     6841 tacattttct ttctcatatg attttatata tgtacaactt tgaatcttct ctatcctata
     6901 ctctgcatct tcaagctttt tggcttcttc ttccctatat tcttcttctg ctatccaata
     6961 tttgatatct tttaatgtat cttctaaagt attttttagc aatgatttta acaactcact
     7021 tagaccaaca atatgagtct cactacagta actatttaac tctaaaagaa gacgtgtttc
     7081 ttctaatata ttcactttct gtacgccatc accatatgaa agttcttgta caaactccat
     7141 aatctctttc atattattag tcctactaca taaatctgta tacatcttat ctaattgaat
     7201 ctttttatct tttagactat ttatagaaga tagaatggaa atagaatctt ccataccttt
     7261 tttaacactt tctatataag gaactaaaag tttttctagg tctttatagg ttacatcaac
     7321 agctttttgt atttctattt tacaagaagc agtaagcatc aaccccaaat ctgttttttc
     7381 aaatgtttca ggtagatttt tcatttcatc caacaacttt tctggctgtt tatctagtgg
     7441 attacataat ttaatagata caccatctga taatgctaca tttaataagt acactaattc
     7501 cttacaaccg tcgtaatctt tctcttgact atctaggtct tcttttgctt tttccaatat
     7561 atttcttatt ttttcttctg ttacattacc aattagtgtt ttaggtattt tattgataag
     7621 aaagaagaaa ttttctttat actttgcata atcaccaagc atatctttca aatttttgcc
     7681 tgtttctatc aataatgtat gtctagagtc cagtagggat tgatagtcta aaataactac
     7741 catccctttt acctttgaag atgtggaaat agtttccctt acaagcatag caccagcaat
     7801 catatactct gctccacggt tatcaaaaaa acctgggcaa tcacataatg aaaaatctct
     7861 atttgcaata tctacaactt ctggacataa agtcttagaa tatacaccat ttccaatttc
     7921 gacagctgaa tcttttgcag ttatatatat ttgtccagtc tcttcatccg ttttttcttc
     7981 catcacacag ccttttaaat aatttataca agtactcttt ccaacgcctg aagcacctaa
     8041 gaataacaaa attgactttt cttttaagtc atattttcta agctcgtctc ttccttcttc
     8101 caaaaggttt acgatttcac tataaagttt atttgccata catccatccc tttctatatt
     8161 tcggaacaat agccttgata tttttcttta ataagattct cttctacctt tgaaacagct
     8221 gttgataact tagtgtttgg agcattaaat cctccattgt ctcctctatc tccatattca
     8281 gcgtattctc ttgctggtcc tgttttacct tcattccagc ttccaccact aaattctata
     8341 tgatatttaa ttatactatc taacttataa tcccaacttg taaaatatcc tgtatagtat
     8401 acagtcttat ctataccact tggagaaaat gaaagtgtat tatatccgtg atagccacca
     8461 cttcctccat atccacctaa acctggtcta ccatttggac ctgcttctcc atctttcact
     8521 gttatatcac aatgattaat tattctattt ttttgttcct ctgaatctga aatgaagtaa
     8581 atattacctt tcttaccagc atttccacct cttccagcat ttccaccagc tccacctttt
     8641 tcaccttttt tgccacttgt acctttttca aaataccctt taaacatctg tccgtggaaa
     8701 taacttgtat atcctgcatc agctccatta cttccagaag ctcctgtttc accattttcc
     8761 ccatctattc ctttagaacc atttcctcca tttgcaatca gtgttagacg ataatttcta
     8821 atttcttctt taatatataa gtaaatatta cctgcatctc taccatcttc accatttttc
     8881 ccatacatag aattatctac aagttctccg ctctttccac ttacattaat ttttacatta
     8941 ctacctacat caagattgtt agaagcaaga ataatatttt tctctgatgc aaagttcatt
     9001 ccaaggttat catccatata aatagtatct ttagcaaaga acataaatgt agaaacattg
     9061 tcatctactt ttctattttt gaaatattcc aatacactgc taattactaa gtttggaatt
     9121 aatgctctta tttcaatacc tttcttatca ttaatattaa ttgtttttac tataaatgga
     9181 tttaaaatgt atttaccaat aaattctcta attatctcat taccttcttc tccatatgca
     9241 cttaatcttg ttatactgtc ttctaactct tcattttgtg taactgtaac atttgtcaaa
     9301 attgaattaa aatcacttac actatataca taatcctttt tatttgcaac ttccattaat
     9361 ttttctctat ttggtaatgt aaatacctgt ttttgcactt cataagaaac taaaactttt
     9421 tctaaattct ttcttattat aatgaagttt tgttccatta tcttcttact tgccttttct
     9481 ataataaatt ccatatgtac agaaggtaat aaaatttccc cagttccatg ttctgtatat
     9541 ttacttaaag catcaattat atctaagttt ccttttatct tattttgtaa tttcatgcaa
     9601 ctatcttgta agaataatcc actttgtttg gctaatacgt tgataaatct ctctatagta
     9661 agacttgttg tactttccaa ccagttttta aatactttta caacctcatc atattttgct
     9721 atttcttgaa tcattgttat acttccaata atattgataa aatcttctgt atacgcctta
     9781 acataatctt ctaggctaga tacgatattg ttttgaattt cttttatcaa cgtatctaaa
     9841 actaatcttg catctgcact aattggatat tggaatgctg atgtcttatc tttaacttca
     9901 tctaattcta gtacttgctt taataagcct tccctctcat ctgatgcaag tggcttacaa
     9961 agatgaatat tttttccatt atcttttagc aattcttcaa ataaccaagc tttttcctct
    10021 atattactgt ctaaattttc gcataattgt tcatattttc tttttataac attatatatt
    10081 tgtttttctg ttcttcctaa gaaacttttt gaaatgaaaa aagttatact acccttaaaa
    10141 ttttctttat gaagacacat atttaactgt tggaacaggt ctagtacttt tgtaagtcta
    10201 gaatctaata tatctgcttc tggtatcaat acaattactg cacatataga tgcagaattt
    10261 ttaaatactt cacctaatga aactgctgca ctaatatcat gggctactcc cctattgtcg
    10321 ctaaaacctg cagtatcaca taattttgca tccattcctt ctattggttt tctcatattt
    10381 acaatgttcg ggtataaagt ttgagaaaca gctcctgaac caatctctgc cacagggtct
    10441 aagcaatcaa tatatgatat accagtatct tcatcttcta cttccatcat tacacatcct
    10501 gcaagatagt taattgcagt acttttgcca catcctgtcc caccaagaat tagaattaaa
    10561 ttttgatttt cagccttatt tattgctact tttccttcat ttaataagag tgttatttct
    10621 tctaaaagtt gattgttatt ttctggcatt tattttcccc cttggttata tattttattt
    10681 aaataaagtt taatccctta agtagattgc aaagttttta tactatattt aaatttgttt
    10741 tttatttttt atttttaata aatttgcatt ttcactaaat ttgtattttc actcaataat
    10801 tattcttctt tgtttattaa aataactgtg ttgtataata taatctttat caacacagtt
    10861 attttattta atgcgcttat tttattgtat tttatgtaat acagttgttt tttatattat
    10921 atgtccatta ttttcctgtg gttattacat atagattttt aattatattt gtaccattaa
    10981 tttaaacatt cctatactac tgcttaagtt ttacacctat accagcttga atattttatt
    11041 tttatttgct taaatagttc aactatactg gtttaaatag gttatcttta tttgcttaaa
    11101 tagttcaact atactggttt aaataggtta tctttatttg cttaaataag ttcaactata
    11161 ctggtttaaa taggttatct ttatttgctt aaattagttc aactatactg gtttaaatag
    11221 ttttccttta gtttaaataa ttcacctata cctacttgaa tagttcactt ttatttgctt
    11281 aaattctata ccaatactgg cttaaacatt atactaccat gattgtattg tctatcttca
    11341 actacaataa aaacaggttc taaatcttta gtagatgtta tttttaattc taaaaaaaca
    11401 ttatcagttg gtaatatttt tacatcataa ttatttaaat tattattcgt gatactatta
    11461 aatacatcac cttgacgttc aaaaaaaaca ttatcaacta ttacaaaata aacatcttct
    11521 tttctactat tttcaacctc tatagtattt tgtttagtta catttagtct catcttccac
    11581 ctcctattaa tattacttaa ttatattaat acttgtaatt gttgcattat gttcatcaca
    11641 aggaagaggt ttaaaaacaa ctatctctgc taaataattc tgtggaggat ttgtaactga
    11701 agtagcatta caaataaatt taaaatttgt tcctgctaca acttgagtag aaacaataag
    11761 tggttcataa ccaacccctt ctaacattcc tacagcttca ttaaatactt ttaaatcttc
    11821 ttcttcgaca gctctgaatt ttgaccatcc accaacaaaa agactcatat aaaaacctcc
    11881 tttatatata tatttattta tatatatagt agtaccaatt ttctcaatat tcaatatttt
    11941 ctgtgtaact ggtcaatgaa aatgcataac tggataaaaa tacttataaa ataacttaac
    12001 tttacatata aatagcttaa tttacatgta ataagattat tctacaatat gaaatacatc
    12061 ttattttgaa tatattctaa atacaataat ggatattttc aattattata cttagaaaat
    12121 atccattact taatttgttt tatttaaaat cacatagaga ctttattatt ttttttattg
    12181 catgaatagc tatttcaggt atcataaggt gatatacccc ctccttgaaa ccacaaaaaa
    12241 cataacaaac tttacgaatc ttgtcttaaa agaccacagg tacattcctt atttgtggat
    12301 tggagtagcc ataacaagca aattttacaa tttttatgat gacaaaaatc aataaaatcg
    12361 cttagtatat aacacgaaat actacttgtt gctattaaga atagttagat aggaagtatg
    12421 cattatacca ataaaggaat caataaagaa agttaaaaca tccctacaaa ttaaaaatct
    12481 gttacatttt tctatattta aacgttattt acttacaatt ggagaaataa caggttttcc
    12541 ttctcggtca agtaaaggcg taaatccggc tgcatggcca taatagtgga atacatagtt
    12601 cacacctgtt tctttatcca cccaaatctc cgttgcattt atttttcctt gtgaatatac
    12661 tttttcaaat ctatcttttc ccattttata atcctccata tttttgattg gtctatgcct
    12721 tcttcactta agtagaaaaa atacataact tattttttta attctaatat gagcacgtaa
    12781 aaatgtcaaa cagcttattt aatttcataa cttcatgtta ctgttcgtaa ccttgtctgt
    12841 ttacttacac tgcattacag caccatttta tcctttacat tattgtattg cacacagtaa
    12901 aaatgcttac tttattggat ttttcaacta aaaaaaggat tttttctcca cagaacattt
    12961 actatttttt ataatatatc taattttact ctcatatcaa catttaatga agatataacc
    13021 tttttcaaat attgtaaaga ctccttaaaa atattagaaa aagattctgc aaatctctca
    13081 acatctcctt tttttaaata cctaagtatc tcaaaacata catcaaaacg tgttttcaaa
    13141 atttcattat attcttcatg aaaaaatctc tctaaataga ttccaaatat catatattgt
    13201 tttaaatgta aaaatgtttc ctgtaataca taaatttgtg tataatctac tatcttacaa
    13261 agtaaaacaa aatctggata aaccttacac tcttcacaat tattttgtaa gtaccttaaa
    13321 tgagtttcta ttttatctac agctatcaaa tccattttat ttcctagtaa aagtactaca
    13381 tcctcaagag taatcgtaac aaactccata gcatccaaaa gcaaactcaa ccttttagct
    13441 ccttcttctg tatttatata atcatcatca tattcagaaa aaagtgttat atatgttccc
    13501 ttgccattaa ttgttttagc aattcccaat tcatttagct cattataggc acttctaact
    13561 gttggaagag atacattata tctattaatt atcgaagtaa ttgatggtag acaatctcct
    13621 attttaatat tgccttcaaa aatttgacaa attacatctg aaacaattaa gtcatataaa
    13681 taacattttt ccaacgttac agaattaaaa ctacttgtat ctatactttg tggagcacga
    13741 ttttctacca ttttaagtat ggattctcta caagctttat acatatatgt taatagtttt
    13801 ttaaattcaa ttagattccc gtccaatcca tataagtaag tttttcttac ctgctctatg
    13861 tatttattac gaaaaaagtc tatacaacaa ttatccaagt tatccaagtt atcgaaatta
    13921 taggccaatt gtgaaataat gacatcatgc tgaataatct cacaaaaatg gtgaatcagg
    13981 aaattatttg ttttacttat tagtttattt atatatttta gcctttgttc tttaaagact
    14041 aaataattag aactattttt ttccatacaa tcgataatct cgtttaattc ttcaaagtct
    14101 ttagatgtaa atcttttaat attgtacata gcaatagaag gaaatattaa actaaaaatc
    14161 ttatatattt ctgatatttg taaaatcttc tcatataata agtcagattc tattgattgt
    14221 ttactcgtac tctggattaa aactggtcta gaaccagctt ttgtctctat aaagcccttt
    14281 tctgagagca tttttattac agtttttata gtattttttg ataccatata ttcttctgac
    14341 atgcttttta tagaatcaag cctatctcca atagtaattt taccagtata aatttgttga
    14401 attatatgct caaaaataat taaatattgt ggttttctct tcatattttt cctcttcgat
    14461 aaattagatt tattgttttt tgtaaggtat atttactttt atttaatagg gcaaaatagt
    14521 tgcttatttt ttcctcataa tctataatta atatagacca tttatatata tggtctaagt
    14581 tttataatac tgtaatgcag taaaatagca tattgtagct atactacagc aaattaacat
    14641 accattgtag tatattaatt ggaataattt taagtacttc aaaattccag caaaacatat
    14701 atgtgttttt gatattatgt atattcgtta atagaatagc tatacttgtt ttacataata
    14761 aattatatga tacttgctat atgtaattac tatatttaat acacataact attatactta
    14821 ctatatgtaa ttactatatt tgatacacat aattattata cttactatat ataattacta
    14881 tacttggaca cataactatt atacttgcta tatgtaatta ctatacttta tacacatagt
    14941 tattatacat gctatatata attactacac ttggacacat aactactata tttactatat
    15001 gtaattatac tatacgtgga catatagtta tcatacatac tatatgtaat tactatactt
    15061 ggacacataa ctattatatt tgctatgtgt aattatacta tacttggaca tatagttatc
    15121 atacatacta tataattact atacttggac acattatcat acatactata tgtaattact
    15181 atacttatac acatagttat catatatact atatataatt actatacttg gacacatagt
    15241 catcatacat gctatatgta attactatac ttatacacat aactattata ctttccatac
    15301 ataattacta tatttggaca catagttatc atacatactg tatattaaga tataatactt
    15361 tttaaatttt atataacaca gacacatgca ataaaaaaga aggtatccat aataagctct
    15421 ttgcatgtac ctaaataaaa actgtaaaat agaatatata aaatctaatc tattttaagt
    15481 aaatacaatt tcactttaaa agaggatgta ctatgaaata ccatatattt ttaatcataa
    15541 ctactatatt aataatcgaa ctatgtaaca tttacatatg caaaatagca tcttttagaa
    15601 acaaaaatat aactatacat aagaattaca aatatatttt tttaataaaa ccattaacct
    15661 tcttgataat aatattcaca aaattaccat taccactata ttatatattg ttttacatag
    15721 taagtcataa attgctctta agcagaaaaa gttctactaa caaccatttt attcccaata
    15781 tggcagtaat aagtttttgt attttacacc ttattactgt aagtacaata tcacttgtta
    15841 aaaatacaag tccagatata atatttgcaa atatgaccta taagttaatt agtattttaa
    15901 tagcttctat tttagatttt atcttatggt actctatgaa taaaataaaa gagcatattt
    15961 atataataac tcaaaatcaa atagagttca gtctattttt aaaatttatc tggttcactt
    16021 taggatatat attatttgat agtatacaaa ttacatttgc tccaccaagt tctctaactt
    16081 cctactttat attgggaagt aacatattat taatcttaca actaattata ttcatcaatc
    16141 atatatattc cattgaaaaa aatatttatt tggaaaaaga acataataga ctaaaaatga
    16201 taaaacaaga acaattacta aaagtaaatt tattaaagaa aaaagccaac attgatgaat
    16261 taacatcagc atataatcga aaatttgctt ttgaaaaaat acagcattac attgataaca
    16321 atttattatt ttcattgata tatatcgatt tggataaatt aaagacgata aatgatacat
    16381 atggacattt agctggggat aactatatta ttaacttctc aagtatagtt attgaaaact
    16441 taggaataga tgacatattt gctagaatag gaggagatga gtttatagtt attatgccta
    16501 aatctaatga aaacaccaca aaagaattta tagagaaaat aagtaaaatt atggataata
    16561 cgatatcttt tagctatggt attgtagaag tttctagcga aaacaaaatg actgtagaag
    16621 aaattattaa tattgctgat attagaatgt acactaataa acaaaataat cataaaagga
    16681 acaataacta aaatgaatat ttacatgagc ttacttgtag tctcactatg ttttttatct
    16741 tattatctct atcattataa tttttttaaa tatctggaca taatgattaa taac
//
LOCUS       DBGJFU010000085        16767 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.085, whole genome shotgun sequence.
ACCESSION   DBGJFU010000085 DBGJFU010000000
VERSION     DBGJFU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.085"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(155..2434)
                     /locus_tag="ACOIAS_002567"
     CDS             complement(155..2434)
                     /locus_tag="ACOIAS_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2702270.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2705..4159)
                     /locus_tag="ACOIAS_002568"
     CDS             complement(2705..4159)
                     /locus_tag="ACOIAS_002568"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGZ2702271.1"
                     /translation="MKLNDIIQGLDIINIKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4323..5192
                     /locus_tag="ACOIAS_002569"
     CDS             4323..5192
                     /locus_tag="ACOIAS_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2702272.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5405..7477)
                     /gene="ptsG"
                     /locus_tag="ACOIAS_002570"
     CDS             complement(5405..7477)
                     /gene="ptsG"
                     /locus_tag="ACOIAS_002570"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HGZ2702273.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVSTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(7780..8622)
                     /gene="licT"
                     /locus_tag="ACOIAS_002571"
     CDS             complement(7780..8622)
                     /gene="licT"
                     /locus_tag="ACOIAS_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGZ2702274.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(8883..10925)
                     /locus_tag="ACOIAS_002572"
     CDS             complement(8883..10925)
                     /locus_tag="ACOIAS_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGZ2702275.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNIMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVIKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATILLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(11077..12048)
                     /locus_tag="ACOIAS_002573"
     CDS             complement(11077..12048)
                     /locus_tag="ACOIAS_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702276.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKKIKSVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(12049..13026)
                     /locus_tag="ACOIAS_002574"
     CDS             complement(12049..13026)
                     /locus_tag="ACOIAS_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702277.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            13245..14804
                     /locus_tag="ACOIAS_002575"
     CDS             13245..14804
                     /locus_tag="ACOIAS_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2702278.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            14910..15869
                     /locus_tag="ACOIAS_002576"
     CDS             14910..15869
                     /locus_tag="ACOIAS_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702279.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            15872..16684
                     /locus_tag="ACOIAS_002577"
     CDS             15872..16684
                     /locus_tag="ACOIAS_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702280.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLIDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
BASE COUNT         5748 a         2652 c         1955 g         6412 t
ORIGIN      
        1 ctacatatta ataaactttg cccaataatt cctaaaataa tatcctaaca atgatttatg
       61 tttttataat agtatatttt tacataaaaa agtgaacatt gattaacaaa gttcactttt
      121 ttataaattt ttatgttata attcacaatt ttatttaatt tgtttctttt ctatttaaac
      181 tatatctatc taatattaat ttttcatatt cttctattgg cattggtcta gcaaataaaa
      241 atccttgggc catatcacaa cctataaacc ttaaaaactc tgcttgctct acagtttcaa
      301 caccttctga aacaactttt atatcaagtt gctttgccat ttgaacaata cttgaaacta
      361 caatttgact cttttttgca tttcccactg ttataaagaa ttgcctatct aatttcaata
      421 catcaattgg catatcttta agcatattta aagaagaata accagaacca aaatcatcca
      481 tagaaatttt aaatccaaca gatttcaaag tttgcattat tttaaataat atttttgtat
      541 tgtcaaatat tgcactttct gtcaattcaa tctcaattaa atttggttca atttcatatt
      601 cttttgttat tctttcaagc tcagaaataa agcctggatt ctcaagatgt actcttgata
      661 agtttattga tataggaaat gtggggaaat tttcattttc ccaacgttta aaatttttac
      721 aaacttgggt aaatacaaac atatccaatt ctgttataaa gccatttttc tcaaaaatag
      781 gaataaatac acctggactt attagacctt ttttaggatg ttgccaacgt acaagtgctt
      841 cagcacctac aattttagta gttgataaat caaccttagg ctgtaaataa actttaaatt
      901 gttcattttt taaagcatca tgcatttctt gttcaatatc acgttcttct atgagagttt
      961 cttttaaatt ttcagcataa atctcataaa cttttaaata ttttcttttt actgtagttt
     1021 ttgcaatcat agctttgtca agacaggtag aaatatttgt ttctccctct tcaacaaaat
     1081 atacacctat ggatggaatt aaattgagtt ctactcccaa acttgttttt atcattgaca
     1141 attttttacg tataagttca catatttcta taattgactc tctattattt ttcttttcaa
     1201 atattatagc aaaattgtca tttgaaattc ttgaaaatac agattgctct ttaaatatat
     1261 tcttaattat ctctgctatt tcctttaata ctctatctcc ttcttcatac ccaaacctat
     1321 catttactag cttaaactta tctatatcaa aataaattaa aactagattc tttttctcat
     1381 gagaggataa aaattttccc ccttcaagat taaatttatt tgaattgcca atacctgtaa
     1441 cgttatcttt aaaagcaaat tcttccaatt tttccttact ctttttatgt ataaatagta
     1501 tataaattgt taatgccgta aagattataa tcaagactac acatgctgat aatgtaagtt
     1561 ttataatagc atatgatttc tcagatatag cagttttagg aacttcagaa aaaagatacc
     1621 aatcattgat tcctattggg gcataactta aataatatcc ttttccaaac atactataac
     1681 ttaatgaacc tgatttaaag ttttttatat ttgacttcat attgttgata ctagattttg
     1741 atattgaatc acttctgcca atgtaagaaa ataaattttc tttagtagag ttggcgtttt
     1801 tactgcttgg atttaaaaca atatcaccat tacttctagt tatataagaa tatccttttc
     1861 cattaaatgt aggtacagaa agtatttttt catacaactt cgtactatgt gtggcaaata
     1921 taactccttt aacctcatta tctttataaa ttggtacaga atatacatta atattactat
     1981 tggtattttt atcatcttct attacatctt ttaatttgcc tgttataacc acttctcctt
     2041 gcattgattt taagaaataa tctcttttag agaagtcttc tatataacca tctgtagtgt
     2101 atgctgtacc atttggtaaa ataactccca ttcttttaaa tgaactatta atagcttgtc
     2161 tttttagtat agaaaggata ttatctactt caaaagactc ttctccttca ataaatattg
     2221 aaattgactc tagtaacttt atatctccat taatttgttt ttttaacaca ttgacacttt
     2281 gatttgctat ttctgaaaga tattgattcg tttcttcacc taatgtctta ttcaaccttg
     2341 aaccataaat caaacaggaa attgtaatta ctaagatact tatgacagca attatggaca
     2401 aaccatacct attatttttt gcatcactta acattatgct tcctgccttt ctcgaatcta
     2461 tgtatgtata atcattatct tatgacaatt attgtaaaca ttttataata atacttattt
     2521 ttccatattt actgtaatta tacaaaatta taaactttat tacaacaata aattaattaa
     2581 tatagatatt agtgtttata ttattgcatt atctatttta caattattta catataaaaa
     2641 aatgtaatat caaagtgaag cttgatatta catttttgtt cttgtatttt gtttaatttt
     2701 tatgctacat cttacttaaa cactcatttg caacttcttt atcatcaaaa tgatgcttaa
     2761 ctttacctat aatttgataa gtttcatgtc ccttacctgt tataattaca acatcatcct
     2821 tttttgccat acttatagct tctgctatag cttgttccct atcaacaaca acatggtaat
     2881 tttctttttt cttgtcaatt ccttcaagta tatcttttat tattgcctct ggttcttcag
     2941 ttcttggatt atcagaagtt ataatacata aatcagaata tttttgtcca attgctccca
     3001 tcaaaggtct cttttcagta tctctatctc caccacaccc aaacacagaa attatttttc
     3061 cttctgcaaa ttgttgagtt gtctttagta cattttcaag tgcgtcagga gtatgagcat
     3121 agtctacaat tacgcttata cctttatcat ttggaactgt ttcaaacctt cctgatactc
     3181 cattagaaag tctaaggcct tctttatata ttggtttagg tatgccaagt acatagcaag
     3241 cagctataac tgctaaagta ttatatactg taaacattcc tggaactggt ataaatatta
     3301 cttcttcata tgaaggtgtt attaatctat aagatactcc atccgaatct gattttatat
     3361 ctctagccat aaaatcagcc ttagtatcaa caccataagt gtaacaaggt acatttatac
     3421 cttttatatt ttcatatatc ttttttccac cttcatcatc tatgtttatt atatttgcca
     3481 tagtagtctt aaaaaataat ttttcttttg cttttctata atcctctaaa tctttatgaa
     3541 aatctaaatg gtctggtgtt aaatttgtaa atatgcctag tttaaagtct gtttcatcaa
     3601 ctctatttaa tgccaatgaa tgtgatgaaa cttccatcgc acagtagtca catccattat
     3661 ccaacatatt attaaaatga tattgtaaat ctatactttc tggagttgtt gaattggaaa
     3721 caatgtcttt ttctccatca aaaattttta tagttcctat aagtccaact ttattcttat
     3781 ttaaagttaa tatctcattt agtatagtag ttatactagt ttttccatta gtacctgtaa
     3841 ctcctataac gttaaatttt tgagatggat gattgtaaaa attatcagct acttttgcca
     3901 tatcttttct tgtatctttt acctttataa atgtacatcc ttttatttct acatcttctt
     3961 caacaagaaa tgcacttgct cccttttcta tggcatcttt tatatactta tgaccatctg
     4021 acacaaatcc ttttatacat ataaataatg taccttttgt aacctttctt gaatcatatt
     4081 gtacattatt aatatctata tttaattcac ccttgatatt gattatatcc aacccctgta
     4141 taatatcatt taatttcata aatgctttcc tccctttaaa ctctttctgc taaaattaca
     4201 ctacatataa tattatatta agaatttttt actatgtgaa tggttaaaaa tgcttgaaac
     4261 attgcaaaaa atgctctata attttctata atataactga ggaaaggtct tctataaaaa
     4321 ctatggataa taaaatcggg tacttaaacg ataactttaa aatatttaat attagagata
     4381 aaaaagatat acaatttgaa taccataatc atgattttaa taagataatt atatttataa
     4441 atggaaatgt aacttatttt gttgatggta ttccctataa actaaagcct tgggatatac
     4501 tctttatcag caataatgaa atacataaac cagttataga ttcaaatgtc ttttatgaga
     4561 gaattgcaat atggattagc ccagaattta tgaaaaaaaa tagtgatgct agtagtgact
     4621 tagaaaaatg ttttaaaata gctacagaga ataaatgcaa tagattgcgt ttaaatatac
     4681 atgatattga atctattaag agttttattg agcaaataaa tatttccgaa tccaatactg
     4741 aatttggaaa tgaaatattg agaaatgcat tatttataca acttatgata ttcttaaaca
     4801 gaattttcct aaaaaatgaa aatatggaat caacagaaga tgttttttat gatgaaacta
     4861 ttaagaaagt attaaactat ataaattcaa atatagatga taagctttca atagatagca
     4921 tatcgtctga attttttata agtaagtatt atcttatgag aaaatttaaa tctcatactg
     4981 gaacgtctat acacaattat atagttcaaa aaagattaat tttggcaaag tcactaataa
     5041 ccaatggtca ccttatgagt gaagtttgta ataaatgtgg attcaacgat tattctagtt
     5101 ttgtaagggc atttaaaaaa aactacgggg tatctcctaa aaattatata aacaataaaa
     5161 atcctttgga tattactctt ttagatgaat agataattta tgtaaatttt attttaataa
     5221 aacttgaagt attagtgtat ttatcttact aatacctcaa gttttttaat gttttaaaat
     5281 atatcaaaat ataatgatat atatttctat tacttaaaat atatattaaa taaattgcat
     5341 gttaactgaa cctatttata tatttattaa atcccacata acttttagtt acactatcat
     5401 atagctatcc tattttttca atatcatctc ttaatatact tactctattc tcctcttttg
     5461 ctgtgcttac tcctttttta atgcttaatt tctgattttg ttctaaattc gtaaatacta
     5521 caggacttat tgttgataga ccattttttt caatatattc taagtccatt ttaactaatt
     5581 tttctcccgc ttttacttca tcccctacat tcacaaatac ttcaaatcca tttccttcta
     5641 aatttacagt ttctatccct aaatgtataa gtatttctac tccacttttt tgggatttta
     5701 ttattattgc atgcttagtt ggaaatataa ctccaatagt tccatcaact ggtgaataaa
     5761 tcaatccatc actagattta attgcaaatc catcccccat cagtttactt gaaaatacag
     5821 aatctggcac ttcttctata tctaatatct ctccatttgt taatgaatca atcttttcta
     5881 ctaaagcatt ttccatattt gcaacttcca tatttttttt cactttattt ttatcaacat
     5941 ttttagtaac agtactttca acctttgttt ttccctctat tatatctttt atatcagact
     6001 ttattgaatc cgattttggt ccaaatatag cttgaacatt gttgccaata accataacac
     6061 ctgctgctcc aagtgatttc aatttttctt tatctacttt atttatatca tttacaacca
     6121 atctaagtct tgttatacac gcatctaggc tctttaaatt gtctttattt cctaaagcaa
     6181 tcaaaatctc tgatgctaat tcattcttag acatttcaat gacttcagcg ttagtttctg
     6241 actcatcttc tcttcctggt gtctttaaat taaattttct tatcataaat ctaaatacac
     6301 tatagtataa tactgcaaaa ccaataccta ccaagatagc caagtaccat ggagttctat
     6361 taggaagaac accaaataat aagaaatcta ttaatcctcc tgaaaatgtg aatccaattt
     6421 taactcctaa taattgcata attgcgtatg ataaacctga aaataaacaa tgtattgcaa
     6481 ataatactgg tgcaacaaat aaaaacgcaa actctattgg ctcggttatt cctgttaaaa
     6541 aagaggttaa tgctgctgat gccattatac ctgctacaaa ttttttcttt tctggctttg
     6601 cttcatgcac catagcaaga gctgctgctg gaagaccaaa catcataaat ggaaatgcac
     6661 ctgtcataaa agtacctgct gtgaaattta ctccatcttt aagttgagca aagaatatct
     6721 tttggtcacc cattacaagc tcaccagctt tatttacata ttctccaaat tgataccaaa
     6781 atggattatg gaaaacatga tgcaaaccaa atggtattaa tagtctttcc aaagaacctt
     6841 ccataaatgc tgctagtgtt tgatttgctt ctataacatt ttttgagaaa ctaagtaatc
     6901 cctcttgtat tggaggccaa ataaaagaca atataattcc tattgctata gcaactaatg
     6961 aagtaacgat tggtacaaat cttttacctg caaaaaaacc tagatactga ggtaattcaa
     7021 ttttataaaa ctttttataa agataagcag ccactatccc tataataatg ccaccaaata
     7081 cccctgtttg aattgttggt attccaacaa tatttgcata catagaattg tctaccatat
     7141 cttctgtaac acctgccata acacccatag atgtattgag aattaaaaaa cctactatgg
     7201 ctgccaatgc tgcaacacct tctccttcag ttaaaccaac agctacccca actgcaaata
     7261 taagtggtaa gttattaaat ataatttgac ctgaactttc cattatactt attataagtt
     7321 gaaatgtagg attcgccaaa gctggaaaat gagccaacaa tgcgtctcct gctaaagcat
     7381 tactcacgcc aagtaatata cctgctgcag gaagtaatgc aactggtagc attagagatt
     7441 ttcctacttt ttgtaaaaca ccaaatactt ttttcattta cttttctctc cttattattt
     7501 tatatttttt taaataaaaa agacttaagt ttataaaaag ttacattagt atacttaatt
     7561 ctaaaattaa acttatgtta atataaataa acctatatta acctaatttc agaattaaaa
     7621 ataaactatt ataatttttt tataaacata agtcttgcct gcattatcag taacaatctt
     7681 aatgttttaa aattattatt ttatattaaa ttttaaataa tatattaaaa ttaaacataa
     7741 tatattaaat ctatacattc tgtattaaac tactctttat tataaattca gtacagatat
     7801 aactcttttg atatgtaatg ttaaataaag tagttcatct tgacttacat agtaatcata
     7861 attcttttct acatgagttt ttattttata tgcacaatca taatcctttt catactttac
     7921 tttaattata tctataattt cattatttgt atcattgatt ttttcttttt ttactaatcg
     7981 ttttgcaaag aattttaaat gagtcagaag cctatcataa tttatatcat cttctttaaa
     8041 ttcaactcta taataatatc ttataatatt tagtatgtct tttacaattt tagtaattaa
     8101 tagtgattcc tttgaacttc ccttatagtt tgaatttact atatgcattg ctataaaacc
     8161 agcttcatct actggaaatt tcacatcaaa ttctttattt atataatcta cagcccattt
     8221 cccaatctca tattcttttt tatgtattct ttttatctca tgtagtaaat cattctttat
     8281 agttatgttt tcatgatatc ttttaattgc aaatgcaata tggtcagtaa gtgctacata
     8341 aatatattca taaagcttag tattcaagtt ctcctctgca taagcaatta tttcagtact
     8401 aacctcaaaa aatttctcat caacctggct tgctagttta ctaattttat ctaaaaagct
     8461 ttcatcttgt attacaaaag ttctttcaat tttactttta tctatttctt gacctttctt
     8521 tttttgaaac ccaattccac atcctgttaa tattaattct ttttttatat tttcatctaa
     8581 aactaataca acattattat tataaatttg ttgaataatc ataatcaata tctctcccaa
     8641 aatacaattt cccttattca taaaaaaata cctaaattaa gatacaattc acctgtatac
     8701 cttaacctag gtattgcctg cttaccagta acaatccagc ttgttaatgc ttatatattg
     8761 tattcaattt atggttttat tctattataa ataaatatat atgtcaagta aattattgaa
     8821 aactatttcc tattttttag tattaatttt aacatcaaaa atcaaatttt attccattat
     8881 atttatgatt ctatcatcat catttcatca tagagaatat tttccaataa ctctttagcc
     8941 acttctcttg atatttgctt agtttcaact aattgttgaa cactgtctct ttgactttgt
     9001 atcgccatta ctctgtagta ttctactctt gcctcaaact tatcatcatt ttgaatcttt
     9061 ctagtaatcc aattcaaata acgattatag aatctgattg ctattaaggc ctcatgtctg
     9121 ttatcttcat cagtattttt atttataaat gatatagcag ttttagtaga aagtgcatat
     9181 gcatggtgaa actctttgat tatttcttta ttctcagata ctatttttgt aattttttct
     9241 tttacttctt tctttacttt tctatttata atccacatgc ttatttttaa ttttacaatt
     9301 ttaaatatag atttttgaac agattttctt gattttgtaa tataaaatct atacaattta
     9361 aaggctctat cagaaatttt accatcttct aatagtttca aaacagctaa taatccctgt
     9421 tggtctgctt ctgcaatcat catacttata agtttattat ctggagtatt tagtattcct
     9481 ttttctgagt ctaccaactg ttcatttaaa tcatgtataa ctggttgtac ttcttgtgct
     9541 ttttcattac taagttttaa aatggcttct ttcaaaatta gtttatatgc ctctttatca
     9601 gataataaag gttctgactc ttgtacgtta ttttttaata atcttggcag taaaattgtt
     9661 gccaatacta aacttataag aataacttct gatgatataa ataaaacagt atctctcatt
     9721 ggaaatacat ctttatcagc caaataaaaa ggtatagata aggcagttgc tagtgtaaat
     9781 gtaccatgaa caccacaagt agcaactatt agtgagtatt tacatttaga aatactctct
     9841 tttataactt cttcatcctt aaaacctaaa aaacctacaa aaaaattatt taaaggattc
     9901 tttttaggtt ttataaaaga attatgccat aaatataccc atataaatct tataacaaat
     9961 aaaataagta caattaaagt agaaataaaa gtcaattcca atgccatatt ttcattttta
    10021 ctactaagtc ctgtatatat acttggtaac aaaaaaccaa gcagtgtaaa gacaaatcca
    10081 ttcaatacat actccaaaac ataccaagta ttgtctgata ttatccttaa tttagtagtt
    10141 gtattttgta aatgctcttt ttcaattcca tgagctatcc ctgccattac aacagctagg
    10201 attccagata ctcctatttc ttctgctaca aaatatacaa aaagtggagt cattatttgt
    10261 ataactatta ccattggaat ttcttccaaa ttccacttgt gtaaactaag tcttattttt
    10321 ataattatgt atcctaaaat acttcctact attattcctc caaaagctgt aatcataaat
    10381 tgaatccctg cctcttctat tgaaaaaact cctgtaactg tagcaagtac tgcaacctta
    10441 aatgctacta ctccagcagc atcatttaat aaagattcac cttcaagtat actcattata
    10501 tttttaggaa aatttaatcc ttgtgtaatt gattttactg ctactaaatc agttggagat
    10561 attactgctg ccaaagcaaa tgaaacagat aatggcatcc caggaataag aaaatgtata
    10621 aagattccac ctgcaaacac ggttattaaa actaaaccaa atgcaagaag caaaattggt
    10681 tttctaagct ctaatagctc ttttctcgat acattttgtc cttcactgaa tagtaatgga
    10741 gctatgatac atattacaaa tatttctgga ttaaatgaaa atgagaattt aaatggcaaa
    10801 atagacaata taatccctag tacaatttga tatattgcaa gaggaatatt actccacttt
    10861 ttgctaaata cagttgaaat cgttgtacaa accaataatc ccaacaatgt tactaaaatt
    10921 gacataacta cctctctttt caataataat attatagatt aaacttatat atcaactaac
    10981 ttaatattaa cagcatgaaa tcccatctta atcaaaaaaa ttttcagttt attctattat
    11041 tcttattata tacatcaaat ttaatttttg aaatatctat actagatgac atgcaacaaa
    11101 atgatttctt gatatttctt ttaaaactgg aatctctttt ttgcatattt ccttacaata
    11161 agggcatctt gtatgaaact tacatccact tggtggattt gttggagatg gtatgtctcc
    11221 ttttaatata attctatttt tcttaattgt agggtcttgt attggtactg cgctcataag
    11281 tgcttttgta tatggatgtt gagaatgtaa gtaaagttct cctttagttg caatttctat
    11341 tatagaacct agatacataa cacaaactct atgtgcaata tgatttacta cactcaaatc
    11401 atgagatata aataaatatg aaagtttata tttatcctgt aaatccataa gtaaatttat
    11461 aatttgagat tgaatagaaa catctagtgc agaaacaggt tcatcacaaa ctataaattc
    11521 tggatttaat gctaatgctc ttgctatacc tattctctgt ctttgacctc cagaaaactc
    11581 acctggtttt ctatttaaat ggtacttaga aagaccacaa ctttctacaa catttaatat
    11641 attatctttt atttcattct ttggcactaa attatgttct ataactgcct cggatattat
    11701 ttgttccaca tttaatcttg gatttaatga actatatggg tcttgaaata tcatttgcat
    11761 tttgggtctt aactttctaa gttcttcctt ctctaactta tatatatcta caccattaaa
    11821 ttcaacttta ccttcagttt tttcaattaa tctaagaatg gttcttcctg cagttgattt
    11881 tccacatccc gattctccaa ctatagccaa agtttctcct ttttttaaat caaaagaaat
    11941 tccatctact gcctttacag atttaatttt cttatcaaat aatccagatt taacttcaaa
    12001 gtacttttta agaccttcta cttttaataa actctcttct tttttcaatt atatctcctc
    12061 ctttttatat aaccaacatg caatactatg accactattc aattctatta aaggaggtat
    12121 ctgagttcta catttatcac atactttttc acatctatca ctaaaataac atgaatctgg
    12181 cattccaatt ggatttggta cttgtcctgg aatcgaatac aatcttttat ctttgttttt
    12241 tccaagagca ggctttgatt ttagaagtcc aatagtataa ggatgttttg gatttttaaa
    12301 taattcaaca acctctgcca cttctataat tttgccagca tacatcacta atactttgtc
    12361 acacatttct gcaactactc ccaaatcatg tgtaatcatc attatagatg tgtttaattt
    12421 ttcttttata tttttcatta tatctaaaat ctgagcttga atagtaacat ctaaagctgt
    12481 tgttggttca tcagctataa gtaattttgg attacatgat aaagccatag ctatcattat
    12541 tcgttgtctc ataccacctg ataattcatg tggatatgaa ttatatattt cttcagctct
    12601 tggtatttct accagtttta tcatatcgat tacctttttc ttagcttctt cctttgataa
    12661 atcttgatgt atcataatac cttcaatgat ttgacttcca acagtaaacg ctgggtttaa
    12721 agatgtcatt ggttcttgaa aaatcattga tatctcattc cctcttattc ccataagttc
    12781 atcttcattc atctttaaaa tgtctttgcc tcgaaagatt atttctccac cttctatttt
    12841 tgatgttgga agtaacttca tcaaagacat ggctgttaca gacttaccac atcctgattc
    12901 tccaactatt ccaatagttt caccttcatt tatattaaaa cttacttcat ttacactctt
    12961 tacaatccct ttatctgtat gaaaataaac ttttaaatct ttaacctcaa ttagtgcttt
    13021 actcattatt ttctcctctc agcaaaatta cttaagataa catacacctt ttgattgtat
    13081 tttataaaaa tttaaattac aacattttct tacattttat tatttttttc tattaattat
    13141 aataatataa tagttaattt ctatatatat tgatttaata atgattacta ttatacaata
    13201 aaaaatatgt gttaaatatt attttatgga ggttttataa gaatatgaaa tttaaaaaat
    13261 tggcatcatt aatcttagtt tcatcattaa tgcttacatt tacagcctgt gcttccaatg
    13321 ataaagacaa gtctagtgga agcaaatctg gtggaactgt tgtaatacct atggcaagtg
    13381 acccagatat aatcaatgga gcttttgcaa acgttaaaga agactctctt gcatcaaata
    13441 tagtttatgc tcctctttat acttatgaaa aaggaaattt agtaaattat ttagcagaaa
    13501 aagtagattt taaagatagt aaggaattaa ctataaaatt aaaatccaat ttaaaatggc
    13561 atgatggaaa gccaataact gctgaagatg tcttatttac atttaataca gttttagacg
    13621 aaaaacaaaa ttcaccatca agacaatact tattggtggg tgagaaacca gttaaagtag
    13681 aaaaaattga tgatttaaca gttaagatta ctttaccaac tgcaagtgaa tctttcttat
    13741 atggtatatc taaaatttct ccaataccaa aacatgtgtt tgaaggagaa tcaaatatag
    13801 ctaagtcaga aaaaaataat aatccagttg gttctggtgc attcaagttt aaggaatgga
    13861 aaaaaggtga aagcatagta tttgaaaaga atgcagatta ttttggtgga gaacctaagg
    13921 cagattctat agcgctaaaa ataattccta atgaagcatc tcaagaagca gctttaaata
    13981 atggcgaaat ttcacttatg aagacatctg ctgaaggata tgaaaaggca aaatcaaact
    14041 caaacttaca aacttatact tatagtgaag aaagattaaa ctacattgta tttaaccaaa
    14101 atatatcaaa catggctaac aaagaagtaa gacaagcctt atcatatgcc ttaaatagaa
    14161 atgaaatgat agaatcagca tatggaaaag aaggttctgt acctgctaaa tctatattag
    14221 ttccagaagc agacttctat acagaagaag gtgttgaagg atatgaccaa gatacaaata
    14281 aggctaaaga cttattggat aaaagtggtg ttaaaattga taaattaaaa ataggttata
    14341 acacaggtag atttgggcat aaaaactatg ccttagtagc tcaacaagag ttgaaaaaaa
    14401 ttggtataga agctgaaata gttccttatg aaagtaaggc attcttcaat atactattta
    14461 gtaatagcac tgaatgtgat atgtatgtaa atggttatgc ttggggatta gaaccaaacc
    14521 catatagagg tatgtttgaa actggtcaat attgcaacca aactaaatat tctaatgctg
    14581 aaatagatgc tttatgggaa aaaggattta ctgagttaaa caaggaaaaa cgtgaagaaa
    14641 tatataagca aatacaacag gatatatcta aggatgcgcc tatttacact atagattatg
    14701 aacaaaattt aatggcagct caaaaaaatc taaaaggaat aaaagatgct aaaccatctc
    14761 ctgcaatatt atttgaagat tggtctaaac tgtatgtaga ataattgatg taaaatttta
    14821 tattattaat tagaaaagga gatgtctaaa aataatattg ttttattatt ttaaaacgag
    14881 acatctcttt taattaggag aatttactta tgttaaaatt aatattgaat agagtaaaag
    14941 ttttaatacc tatgttaatc ttaatatcta tattatcatt tggactttta gaattagctc
    15001 ctggtgaccc agcagatgca tatataaatc ctttaatgtc ttcacaggat attgaaaata
    15061 taagggttaa tatggggctt gataaaccag tatatataag atatttaaac tggttgaaaa
    15121 acactttaag tggaaactta ggtatttctt atataaacca tatgcccgtt acagaacaaa
    15181 ttatggaaaa aatgggaaat acatttattc ttatgggtac ttctttgata ttttcaatat
    15241 tggtagctat acctcttgga atatttttag ctgtaaataa aaattcaata actagtaaag
    15301 tatcatctgt ttttaattat ataggtgttt ctataccttc tttttggata ggtatgatac
    15361 tcatttcaat tttttcagtt aaacttaaca ttttcccatc aggaggtatg catactattg
    15421 gaaatgatag tattggagac ttagtaaaac atttagtttt gcctgtaata actttaggtc
    15481 tatataatac tgctattttt acaaattatg tagaagctag tgtaaatgaa caattaaaaa
    15541 aacaatatgt tattacagca agagcaaaag gattatcaga aaaagtcata ctatttaaac
    15601 atgtcttgaa aaattcactg acatcattgg ttacaatcct aggcatgagt atacaaaaat
    15661 tagtcactgg tgcatttgtt acagaagtag ttttctcttg gccaggtatg ggaagattaa
    15721 tgattgattc aattttttca agagattata ctgtaattat ggcaataact atgttatcag
    15781 cactattttt aattttaggt aatttagttg ctgatatact atacttatta attgacccta
    15841 aaataaaatc tagcaaagga ggattctagt tgtgtttaca agtatcttaa aaaaattaaa
    15901 aaaagataaa tcctttgctt ttgcatgtat tactcttagc ataataatca tattaattat
    15961 agcttctttt atattaaata tagactctaa tgcaattgat actacttcta aacttcaaga
    16021 acctagctta aagcatatct ttggtacaga cgaacttggt agagattact tctctagagc
    16081 tctttatggt ggaaggattt cacttagtgt tggtatttta tcaatgctta tttctgtagt
    16141 atttggtact gctgtaggtg ttattagtgg atatattgga ggtaagctag accaattctt
    16201 gatgagatta atagatatgc ttatgagtat accaagtttc ttagttttga ttgtattaaa
    16261 tacttatcta acacctaaag tttccactat tatactaatt attggattct taagttggat
    16321 ggaagtttca agaattgtac gtggcgaaac attaaaaata aaggaaaatg aatactgtct
    16381 agctgctaaa gctactggta tcaagactag aaatatacta ttaagacata tagttttaaa
    16441 cttaaaagag actattgtag ttgctggttc gttaaatatt gctaatgcta tcttaactga
    16501 atctgctctt agttttttag gtcttggaat ccaattgcct cttgcatcat ggggtaatat
    16561 gcttcaagat gctcaaagac atatttttga caagttatat ttagctgtat ttccaggatt
    16621 acttatattt ttaacagtca tgagtttgac tataataagc aaaaaatttt ctagtaatac
    16681 ttaacaaatt ttatatagaa aataaaaaca gtgattatct aaagatatac actgttttta
    16741 tttttcgttt aaaaaaatac atatttt
//
LOCUS       DBGJFU010000086        16623 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.086, whole genome shotgun sequence.
ACCESSION   DBGJFU010000086 DBGJFU010000000
VERSION     DBGJFU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.086"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(136..306)
                     /locus_tag="ACOIAS_002578"
     CDS             complement(136..306)
                     /locus_tag="ACOIAS_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702281.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(495..716)
                     /locus_tag="ACOIAS_002579"
     CDS             complement(495..716)
                     /locus_tag="ACOIAS_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HGZ2702282.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(800..2026)
                     /locus_tag="ACOIAS_002580"
     CDS             complement(800..2026)
                     /locus_tag="ACOIAS_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGZ2702283.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(2088..3020)
                     /locus_tag="ACOIAS_002581"
     CDS             complement(2088..3020)
                     /locus_tag="ACOIAS_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGZ2702284.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(3109..3636)
                     /gene="hpt"
                     /locus_tag="ACOIAS_002582"
     CDS             complement(3109..3636)
                     /gene="hpt"
                     /locus_tag="ACOIAS_002582"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGZ2702285.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            3865..4953
                     /locus_tag="ACOIAS_002583"
     CDS             3865..4953
                     /locus_tag="ACOIAS_002583"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGZ2702286.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFILKTY"
     gene            complement(5116..6306)
                     /locus_tag="ACOIAS_002584"
     CDS             complement(5116..6306)
                     /locus_tag="ACOIAS_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2702287.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEGRLLAFMEKDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEMGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILKNRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(6384..6587)
                     /locus_tag="ACOIAS_002585"
     CDS             complement(6384..6587)
                     /locus_tag="ACOIAS_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2702288.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(6901..7134)
                     /locus_tag="ACOIAS_002586"
     CDS             complement(6901..7134)
                     /locus_tag="ACOIAS_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702289.1"
                     /translation="MPKELTPTFTVISKACDGDETAISKILEFYDPYINRCCMRPFYD
                     DNNNLRLAVDLEMKGFIKEAIIRKILNFDIKRF"
     gene            complement(7119..7559)
                     /locus_tag="ACOIAS_002587"
     CDS             complement(7119..7559)
                     /locus_tag="ACOIAS_002587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HGZ2702290.1"
                     /translation="MKPSELQEKTCHQFDCAIKTVIRHRAIDFYRAIRRSEKKEMPFA
                     EIMDDLLNSIGTEDTYSTDYVAVFEVCGEKVSIEDEQLARALKAIEERKRNIVLQYFF
                     IGDTDTYIGKMLDCSRANVTKCRIQALDKLKQLIEEEKEKCQKS"
     gene            complement(7834..8469)
                     /locus_tag="ACOIAS_002588"
     CDS             complement(7834..8469)
                     /locus_tag="ACOIAS_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702291.1"
                     /translation="MLEVKNVSYAYKTKKDKTILNNVSATFEEGIFYTIVGPSGAGKT
                     TLLSLLAGLDTAVKGTVLCNGEDITKKGLNYHRKHNISLVFQNYNLIDYLTTVENVKL
                     GGSGNAEKLLEEVGIGKEYWQRNVLQLSGGQQQRVAIARALASDAHVLLADEPTGNLD
                     ETTADEIIALLKKTAHELGKCVVVVTHSKHLAEEADEILEIKNGAISFLSD"
     gene            complement(8483..9928)
                     /locus_tag="ACOIAS_002589"
     CDS             complement(8483..9928)
                     /locus_tag="ACOIAS_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2702292.1"
                     /translation="MNFFGLARRSVFRKPIKSILLLLIVFAISTLLLAGVASKNASIE
                     VQDKTRQAIGAGFLLERNAEYRTKRVREYSEKIGNDKEGSFGGYHQEKEIINGVETWS
                     GWTTNEFESLDIKDIEKLAKTKGIADYNITTATTPVNPVNFKRIEDKDVDQSVDEGGV
                     SLIGNKDMKLDRNVLSGNLHIKEGRMITPEDKDMCVISEELAKQNNLKIGDKISFNDY
                     HDTVNSKVSEAEIVGIYQVDQKMSPLMQGDTYRSENVIFTDLRFPEKAEGETSPLYER
                     AYFKVEDVEAYDEVKENLQKVDINWEQYDLIDNNGNSETMSSNFNDLAKVSEMMILVI
                     SVASFVILVLVFLFWLKNRVQEVGIFLSLGVPKFRIIGQIWSEAIMIAVLSLMLSFAV
                     APAVSKVTANYLVSQQVQQMQEEEKNNEGKVSTEYVAPKQDVQNISVEVTPEMYLLDG
                     VSVLVLITASVLVSGIVILKRNPKDILSEMS"
     gene            complement(9932..11155)
                     /locus_tag="ACOIAS_002590"
     CDS             complement(9932..11155)
                     /locus_tag="ACOIAS_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2702293.1"
                     /translation="MKFYQLGFRYLQRKKGKTILLLIVLILVNSMILGTSMILRTTNE
                     SKQAMQEKTNSKIVAEITSKDSKITENEVNKIETLEDVSSINRIGRQEVFPNDFAPVT
                     LNTSTNEENLKIALLSYDDLEKDSPFSEMQYRLSSGDYIKHEKKGAVINANLANQNEL
                     KVGDTIELSTENGTKVSVQIIGIFMSAGNVEKDQPTETTAVNRIENQVFIDNSTYKEL
                     FKEAEFEKLCVYSKRPEKLDVLETSLQKIFGNDVELSTSDTLYQQMSAPLEQISKVAN
                     LMLVLTLVTGTVVVSLLLCMWMRTRQKEVAIFMSLGKTKSEIFLQTLLEAGSVFTLSV
                     LGATAIGKLFSGVLQNVITGSETVTENLKVVLQIQDIGMLFLLGGAVILVALCCSILP
                     ILRANPKDILAKMEG"
     gene            complement(11167..11664)
                     /locus_tag="ACOIAS_002591"
     CDS             complement(11167..11664)
                     /locus_tag="ACOIAS_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HGZ2702294.1"
                     /translation="MKKTNISKFVAVGLCICALTGCGASPDEKPDTSNPIVNSNTNEE
                     NANGSSENKGNDILESANLIGSVLEFTDNGCSVSQAKETEGGASLQIEASGLENKDNA
                     VSVTYNPDCEFFVATLNKQLGSVTNITTGSISDVKKQSNVYLYGEFSDTLHFNATKVV
                     IARWE"
     gene            11827..12513
                     /locus_tag="ACOIAS_002592"
     CDS             11827..12513
                     /locus_tag="ACOIAS_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702295.1"
                     /translation="MKILLLEDDLELCKNIKISLEKENYMVDCCNDGETAMLYALNTD
                     YAYDLAIVDRMLPIIDGLTIIKSMRRKGISIPILIITAMSTLDNRVEGLDGGADDYLV
                     KPFHIKELLARVRALTRRPANIQLSSKLEYGDLTFDKDSRTLSTPVNSLLLTSKETEL
                     TFVFMQNPETLFSREQLILKIWGSSSEVETGNVDSYISFLRKRLKELKSSCKITTVYG
                     AGYKLEKETC"
     gene            12507..13799
                     /locus_tag="ACOIAS_002593"
     CDS             12507..13799
                     /locus_tag="ACOIAS_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2702296.1"
                     /translation="MLNNLYRKLHILFASSVMLIITLVISFVVANTVHTEKINESTLF
                     QRLTTLLIYQVESASSDMDKALKTYEESYHIFSLITDTRGNTIYQSDFPFPTSADNLL
                     HDVEKQISTQPLSQIENSTTSQGGFLEIRGTHHDTYFVIPATIRTANDTVYHATFLYQ
                     TASLTDILQKTLPIYLLIWFLACIVVIVLTRYLLKKSFAPTERILQSQKDFVATASHE
                     LKSPLAVMISNTDMLLDNVSLNEQAKQAVQTIDFECMRLSRLVKDMLLLASSDAKTWT
                     LHKSTINVDTLLITLYETYEPVCMKQNLELKLHLSEESYPAMFTDKDRLFQILCVFMD
                     NAIQHSKNNSLIEIEVIATEKNIAFSVVDHGQGISDEDKQYIFDRFYSGDKSHTNKSN
                     FGLGLSIAKELTQMLNGIIIINDTAGGGATFTVKFPLK"
     gene            complement(13845..14069)
                     /locus_tag="ACOIAS_002594"
     CDS             complement(13845..14069)
                     /locus_tag="ACOIAS_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702297.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTAP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTKI"
     gene            complement(14115..14810)
                     /locus_tag="ACOIAS_002595"
     CDS             complement(14115..14810)
                     /locus_tag="ACOIAS_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2702298.1"
                     /translation="MCTRFVYNGIDKIVGFNFDIDLSVWDHTVITEENRFYIGIKMPD
                     GLYHSFHGINKNGNVGTLLYVHGNENARYIESEDCITISELTEKFIKAEISFDKALDI
                     VKKKKIIYAPDATMQAMLSDKKGRVLIIEPGIGYRYEQENFSLITNYSILKPDITKPY
                     IVSGDTRYEVAKELLAGYDKDFSVNDAFKVLKSVSQKDLWATRVSFVYSTEKNKVYYV
                     LNNDFSNVFEFQF"
     gene            14988..15545
                     /locus_tag="ACOIAS_002596"
     CDS             14988..15545
                     /locus_tag="ACOIAS_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2702299.1"
                     /translation="MKTTKEAVIKATSDLADEKGLNNVSLKAVAQKLNIRTPSLYNHI
                     ESLDNLLLEVAHNGMRDMNERMMKIAVGKIGKEAIKLVSIEYLNYMIEHPGVYETIQW
                     AVWHGTEETATIFNNYLSLLTTLIQSCSLNKDKTLEILNMLTGIIHGYTTLQLGNAFS
                     ASDKVRFELAEAIDTLLVGIFQKYK"
     gene            complement(15592..16503)
                     /locus_tag="ACOIAS_002597"
     CDS             complement(15592..16503)
                     /locus_tag="ACOIAS_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2702300.1"
                     /translation="MFKKNKKQTETVKEPKEKKVRTVKVGTHKKTMIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIEARTQA
                     ISGYLTKELQDLNVDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVSGNVIEPIGRDYLYSELVNPIFTKNGDNVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            complement(16519..>16623)
                     /locus_tag="ACOIAS_002598"
     CDS             complement(16519..>16623)
                     /locus_tag="ACOIAS_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HGZ2702301.1"
                     /translation="GNNQMYHAGDPIGYADLSSSYWQQHLIGAGRVKQ"
BASE COUNT         5004 a         3229 c         2438 g         5952 t
ORIGIN      
        1 acttggaaat gagagaatct ataattatat atcaaaagca taataattta aacctattat
       61 aaaaaggcaa tttatttaat aaaaaatatt tttaatatta aataaattgc ctttttaatt
      121 ttggattaaa tactgctatc caagtgcttt ttctagttta gataaatgtt gtattggata
      181 atattttagt agctctttaa attgttctac tgataaacct tctgttgttg tatatattct
      241 tattttttca tctaagtcac tattaataaa tttgtttttt aaatcctcaa aatttacact
      301 actcatttta catcactcct tatgtaacat attcatttgt attattttat ccaaatacat
      361 aaaatttatt acataaaaat atatctattt aataatttaa taaaaaaaag ctatctcaat
      421 ttagaaatta ctttctatat ttgagatagc tctagctaaa ctaatagcca atagttataa
      481 ataattattt ataactatct gttgcttttt ccagccattt gttgttcagc catttcaact
      541 aattttttag tcatgtatcc acctacataa ccattttctc ttgcagtaag gtttccttta
      601 tcaacttgtt gataattact cataccaagt tcattagcta tttctaattt catttggttt
      661 aaagcctctt ttgctcctgg tactactgtt ctgttgttgt tattgttact tgccatgttg
      721 attaccttcc ttctgtttaa tttattttgt gttgctgata atattatgtg attttaaaca
      781 tttttaattc aggtaatttt tagcaattaa aacctgccaa atatccagta gaaaaagcta
      841 tttgcaaatt gaatccacct gtatatgcat ctatatctat tacttctcca gcaaaaaata
      901 aaccttctac taattttgat tccattgtac ttgaatttat ttcgtttact tttacaccac
      961 cagaagttat tatagcttct tcaataggtc tgtatctctt tactgtaaac tttaaatttt
     1021 ttaataaatg gactaagttt tttctctgct ctctagaaat ttggtgaaca accgtatttg
     1081 gattaatttc acttaagttt attattatag gtattagttt ttttggaagt aaatcatcta
     1141 atgctttttc aaatttttta tttgtatact tctgaaaatc tttttgaact cttttatcca
     1201 atttttcctc atctaatgca ggttttaaat ctaataatat agtgtaattt tctttccttg
     1261 tatctttcat tctacaactt gcagacttga ttataggtcc atctaaacca aatttagtaa
     1321 attcaagttc tccaaaatca ctataaactt tcttttgttt gctattaaac acccttattt
     1381 caacatttct caatgataat ttttctaaat cttttgtaaa actttcttgt acttctatac
     1441 caattaaaga tggttttgta tctataattg tgtgcccctg tgaaattgca aatttatatc
     1501 catcaccagt agaaccagta agtggataac ttaatccacc agttgctact acaactgaat
     1561 cacacttaat ttctttttta tcgtttaaaa ttactttttc aattttatta tttttagata
     1621 ttattttttc tactttcgaa tttaataata tatttacttt cttactttta agttgtcttt
     1681 caagagcatt tacaatatca aatgctttat cactctctgg aaaaaccctt tttcctcttt
     1741 ctgttttagt ctttactcca agattattaa acatacttat tacatcatca tttgtaaaag
     1801 tataaaaagc actataaaga aattttccat ttgtaggtac attttctatt aattcctcta
     1861 tttcacaagc gttagtaata ttacatctac cttttcctgt tattgcaagt ttttttccta
     1921 atttatgatt tttctcaatt aatgttacat catagccttt ctcacatgca gttgatgcgg
     1981 ctatcatccc tgctggtcca ccacctatta ttactacctt cttcaaataa tcactctttt
     2041 ctacccttaa tttttttaat ttaaactatt gactctatac cttaaaatca atttaatttt
     2101 aaaagtttaa ataatgctgg agctcctgct attccataaa aaacaactac tgtattctta
     2161 ataaaattca taaaaattat actcatagta gagtcactta tatttaaagt gactattaac
     2221 attgaaataa acaacttaat gccaatatat aacattccta ttgttactat cccaaccaaa
     2281 aacctacata ttcttgaaaa aattatattt ttactcccct tatttttatt actaaaattt
     2341 atttttttgt tattattatc tgtattaaat ttaacaaatc tatcctcaac catatatcca
     2401 aatacaaatc ctactattat accaaatttt tcaaaatatt cagggctatt tagaaatata
     2461 gccactatta taaatggtat aagtaaaact acaagtaaaa tataattttt attatcatca
     2521 atatagtcaa atatttttac aaatacaact gtaaaaaata ttcctaatat ccaacccgct
     2581 atcacatcta ttggccaatg aacagctaaa tacagcctag acaatcctac tcctaaaacc
     2641 ataactgcac ctattaaatg aagatatctc tttttaaata tagtcataat actaacccaa
     2701 aatgttgttg cagtttgagt atgagcactt ggaaaagaat aaccaccagc agtagaaact
     2761 ctcaatgatt caagaccttt tattcctatt ggtctgggag cttttacaaa ctctttgatg
     2821 cctaaattta caacaaaatt tccaattata gcaaacagta ttttctgtcc acatttttta
     2881 tttatacacc agtatataat tgttgtaatt aatattaata acggtgcttc tgtgcttatt
     2941 gtaaagacaa gaaatataaa gttcaaaatt ggatttctta aattttggaa aaataataat
     3001 atatttaact gaatatccat atagatgtct cctttactaa aattttatat aattaactaa
     3061 aaagcactaa ctcccttaga aattagtgct tttgattcac atttaaatct attctacatc
     3121 ctcaactata cctatataag gtaaatttct atacttttca gcatagtcta ttccataacc
     3181 aacaataaat ttgtcttcta taacaaatcc aacatagtct acaggtatat tagcttttct
     3241 tctctgaggt ttgtcaagta gagtacatag ttttaaagat tttggatttc tagtttttaa
     3301 agctgcaact agattactta atgttaaacc agaatctatt atatcctcta caatcaacac
     3361 attctttcct tcaatatcta catctaaatc ttttaatatt ttaactgttc cagaagactc
     3421 tgttgagttt ccataacttg taacactcat aaaatctata tttacatcca aatctataca
     3481 tcttattaaa tctgaaacaa atacactagc tcctttaagt atacctacca ctagtaaatc
     3541 ttcgccttta aagtcttctt ctatcttttt tcctaattca taaacttttt cttttatttg
     3601 ttcttctgtc aacatttttc ctgtaacttt gtacattttg tatcctcccg ctttccttct
     3661 attaacttat atattatagc ataccaaaat atcaattaaa atctttttca tcatttttaa
     3721 acctaaatta acagcttatc catacttttt tgctttttcc attaaaaatt tggaataacg
     3781 attcctttgt attataatct atatgtacat atattaatca gttattttta tcgattatat
     3841 aattttattt ttggaggtac ttttatgagc acaaaccatg gagctaattt atatagttta
     3901 tctagtaagt atggcttttc aaaggaagaa tttatggatt ttagctcaaa tataaatcct
     3961 tttggtacat caagtttagc caaacaatac attgtaaaca atatagatat ggtatctatg
     4021 tatcctgacc ctgattacat agacttaaaa acttctatat ccaattactg caaatgttct
     4081 atagataata tagtacttgg cagtggagct acagaactaa tttcatcatt tatacataca
     4141 attaacccta aacaggctct tttactctct ccagcctatt cagagtatga aaaagagtta
     4201 tctaaaatta attgttctat agaaaaatac tttgcaaaag aagaagataa ttttcatata
     4261 aatttagaaa atcttataaa aactatcaat gctaaagatt atgatttggt agtaatctgt
     4321 aatcctagta atccaactgg atttgcattt actaaagtag aagtgagaga aattttaaaa
     4381 aatacagact catttttaat gattgatgag acttatgtag agtttactga tacagataca
     4441 tactcttgta ctcaattagt tgatgattat tctaatttat tcgttataag aggtacatca
     4501 aaattttttt caacgcctgg aataaggctt ggatatggtc ttatatctaa cactaatgtc
     4561 aaaaatgaaa taaataaaaa tctagattta tggaatataa acataatagc ttctaaaatg
     4621 ggtgaaatca tgtttagtga tttagatttt atatcaaata ctatttcctt aatgaacact
     4681 gaaagagatt acctgcttaa agagcttaag aatataaaaa gcttagatat atacaatact
     4741 aaaggcaatt tcatattgtg taaaatcaag acaaaggaat tgactgctaa atctcttcga
     4801 gaacaattgc ttccacaaaa aataataata agagattgtt gttcatttga aggtctagat
     4861 gagtatttct ttagagtatg tatattaaaa cctaatgaaa ataaattatt aatatcatct
     4921 cttaaagcaa tttttatttt aaaaacttac taaatttctg attatctttt cataagttgg
     4981 taaatgccga tatatcccgt attttagggc tttatcggta ttttcttttt ggaagatacc
     5041 ttgcataagc tggcatttac cagcatgaaa atgcaaccaa aagtagtaaa tgagtagtaa
     5101 atataagctc cgatattaac aagccagtct ttccagttct gcttttgcag attgaaatgt
     5161 gccgtgtgcg taatagccaa gtgtcatggt tatgttagcg tgtcccatga gatattgcag
     5221 agtatttggg ttcattcctc tgtttgccat attcgtacaa taagtatgtc ggaatgaatg
     5281 tggcgtgatg ttcggtagct tgtccgtgtg atacttgtta tacttcttaa tcagccctcg
     5341 caccatgccc tcatagtttc ctgcaacttt aggcaaaccc tcacggttta agaataggaa
     5401 attgctgtaa ccacctacaa tcagcggttg tgcctttcct ctgttcttta gtattctttg
     5461 gattgcttga taagcccgtt ctgtcaatgg aagttcccgt tttccgtttt tggtctttgg
     5521 cgtttcaata tagtagccca tttcgctatc tttcaataac tggtggtcta tattgagtat
     5581 tctgttctgc atatcaatat gcgtcgtcag tccgcaaaat tcagaaatac gaagtcccgt
     5641 ttccagcaga agcacaacct catcataata cttactgtaa atcttgtcct tttccataaa
     5701 ggcaagcagg cgtccttcct gctctggtgt aaggataact ttctgttcgg tatcatcttc
     5761 cagaacatca cttaacttaa attcaaatgg gttctttctg atacagtcgt cttgtatcgc
     5821 catgtaaaat gacgctttca aggaacgctt atagttatca atcgttttat aggcatatcc
     5881 tttttcgctc attctgatag cccattcttt agcgtccgac tgcttaaccg tatcaatcgc
     5941 cctcataccc aatgggtcat ttttcagagc gttcataaga tactgtcgtc ctttttcagt
     6001 agcccttttg atgttctttc tttggctgtt cttcttgtcg tagagctggc agactgtcat
     6061 tttaccgccg actgtgtcga taccgtcgtt aaggtctttt tttatctgtg cttctttttc
     6121 cctcaacgat atatcatcac gctttccagc aggtgtcttg tctgtcggta caagtttcca
     6181 agaatagata aattgtggct ttccgtatat atcggtatat ttataaacgt atcttccgtc
     6241 ttttcgctgg ctctctccat tacgcaaatt gcgattttta ctgtcttttc gtttcacatt
     6301 agacatagtg taaagctcct ttccgtcatg gaatgagccg tgatacgcta ctaatattat
     6361 accatatcta cggctcaatg acattagatt tcgtccaatg tatctatgat tttttcaaat
     6421 tgttttcgct taatctgaat acgattacca ttcacgataa cccagccagc gtcaagattt
     6481 tcttcggcta atttacgcaa tttcttttcg ccaatgcgga agtatttgga cgcttcttca
     6541 atcgtcagcg tatatttttc ccagataggc acatcagtat tgttcataat ctatgcaccc
     6601 cctttactgt gtgtcatttt ttacaagcag gcagacggct ttggaaagct ccgttagtat
     6661 ctcaactatt cccattcctg tgggcagaac cgcaccatgc ggacgtatca ttattctgtg
     6721 aggataggtc gtggcaaagc gacttttcca acggtcgctc tcggatcact gcgtgggtcg
     6781 ctcgctttct tttgaaaagg tcgtggcgta cagtccccgt ggcttgctat ctcacaaacg
     6841 tatctgtctg ctttattcag ttgtcaaaga ccagacgggc ataatcaatc ccgatatgaa
     6901 ttagaagcgt ttaatatcaa agtttaagat tttacgaata atcgcttctt tgataaaacc
     6961 tttcatttcc aaatctaccg caagacgtaa gttgttgtta tcgtcgtaaa atggacgcat
     7021 acagcaccga ttgatatatg ggtcataaaa ttccaagatt tttgaaattg ctgtttcgtc
     7081 gccgtcgcag gctttggaaa taaccgtgaa agtcggtgtc agctcttttg gcatttttct
     7141 ttttcctcct caatcagttg ctttaacttg tctagtgctt gtattctgca cttcgttaca
     7201 ttcgctctgg aacagtcaag cattttccca atgtaagtgt ccgtgtcccc aatgaaaaaa
     7261 tactgtaaaa cgatattacg ctttcgttct tcaatagctt tcaatgcacg agccagttgt
     7321 tcatcttcga ttgatacttt ttctccacag acttcaaata ccgcaacata atccgtagaa
     7381 tatgtatctt ctgtacctat ggaatttaag aggtcgtcca taatttcggc gaatggcatt
     7441 tcttttttct ctgaacgacg tatcgctcga tagaagtcaa tcgctctatg acgtataact
     7501 gttttaatag cacaatcgaa ttgatgacag gttttttctt gtagttctga cggtttcatt
     7561 ttcctcacct cctttcgcta gtcgaaaaga ggtgtctgcc ccttttcata tataccgcgt
     7621 tgaaagtggg ggacagcgta accaaaatcg aaaaaacttt aaaaaatttt tttctggcat
     7681 gaaaaaagcc cgcacaaaac atatagtctg tacgagcttt taaaactgtg ctatattcag
     7741 ttgttataaa aatgccgtta ggttcaaact gattgcgtcg aggtatatct aaaatattcc
     7801 ataatgacaa ctcctttacg gcactattct aatttaatca gacagaaaag aaattgcacc
     7861 gtttttaatt tctaagattt cgtcggcttc ttctgccaaa tgcttactgt gtgtaaccac
     7921 aacaacacat tttcctaatt catgtgcagt ctttttcagc aaagcaatga tttcatcagc
     7981 ggtagtttcg tcaagatttc ccgtcggttc gtctgccaaa agtacatgag cgtcgctggc
     8041 taatgctctt gcaatcgcta cacgctgttg ctgtccgcca gatagttgca atacatttct
     8101 ctgccagtat tcttttccaa tgccgacttc ttccaataat ttttcagcat ttccactacc
     8161 acctaatttg acattttcaa ccgttgtcaa ataatcaatc agattataat tttgaaatac
     8221 caatgagata ttatgctttc tgtggtaatt cagccccttt tttgtaatgt cctcgccatt
     8281 gcaaaggaca gtccctttta ctgctgtgtc caagcctgct aacaaggata agagtgttgt
     8341 ttttcctgca ccactcggac cgacaatggt ataaaagata ccctcctcaa aagtagctga
     8401 tacgttattc aaaatggttt tatctttttt ggttttgtaa gcatagctta cattctttac
     8461 ttctaacatt ttcgtcgtcc tcctaactca tttccgataa aatatctttc gggtttctct
     8521 ttagtattac aattccagat accagaacag aagccgttat caacacaaga acacttacac
     8581 cgtccaacag atacatttct ggcgtaacct ctacgctgat attctgcacg tcctgctttg
     8641 gtgcaacata ttctgtggat acttttccct cgttattttt ttcttcttcc tgcatttgct
     8701 gaacctgctg tgataccaga taatttgcag ttactttgga aacggcagga gcgacagcaa
     8761 aggatagcat aagggaaaga acagcaatca taatcgcttc actccaaatc tgcccgataa
     8821 ttctgaattt cggaacaccg agagataaga aaatgccgac ttcctgcact cgatttttca
     8881 gccagaaaag aaatactaaa acaaggataa caaaacttgc aacagagatt accaatatca
     8941 tcatttcgct tacttttgct aaatcattga agtttgagga catggtttcg gaatttccgt
     9001 tattgtcaat caaatcatat tgctcccagt tgatgtctac tttttgcaga ttttctttta
     9061 cttcgtcgta agcttccaca tcttctactt tgaaataggc tctttcatat aatggacttg
     9121 tttcaccctc tgccttttct ggaaatctca aatctgtaaa tattacattt tccgaacggt
     9181 aagtatctcc ctgcataagt ggggacatct tttgatccac ttgataaatt ccaacaatct
     9241 cagcttctga tacctttgaa tttacagtat cgtggtaatc attaaaagaa atcttatcgc
     9301 cgattttcag attattttgc tttgcaagtt cctctgaaat gacacacata tctttatcct
     9361 ctggggttat cattcgtccc tctttgatat gcaggtttcc agataaaaca tttctgtcaa
     9421 gtttcatatc tttgtttcca attaagctta cacctccctc atctacactt tggtctacat
     9481 ctttatcttc aattcttttg aaatttacag ggtttacagg agttgttgct gttgtaatat
     9541 tgtaatcagc aattcctttt gtttttgcca gtttctcaat gtcttttata tctaagcttt
     9601 caaattcatt tgttgtccag cctgaccaag tctccacacc attgataatc tctttttcct
     9661 gatggtagcc accaaaacta ccttccttat cattaccaat cttttctgag tattcacgaa
     9721 ctcgttttgt tcggtactct gcatttcttt ctaaaagaaa gcctgcacct atggcttgtc
     9781 tggttttgtc ctgcacctca atactggcat ttttgctcgc cacacctgca agaagcaggg
     9841 tactgattgc aaaaacaatc aacaataaga gaatactttt tatgggcttt ctgaacacag
     9901 aacgccttgc caatccaaaa aaattcatgt actatccctc cattttcgct aatatatctt
     9961 ttgggtttgc tcgtaatatc ggaagaatgg aacagcacaa tgccaccaga ataactgcac
    10021 cacctaaaag gaacaacatt ccaatatctt gtatctgcaa caccactttc agattttctg
    10081 ttactgtttc tgaaccagta atgacatttt gcaaaacgcc cgagaacaat ttaccaatgg
    10141 ctgttgctcc taaaaccgaa agtgtaaata cactgcctgc ttctaataaa gtttgcagga
    10201 aaatttcact ttttgtcttg ccgaggctca taaagattgc cacctctttt tgccttgtcc
    10261 gcatccacat acataacagc agggaaacaa caacagtacc agttaccaat gtaagtacca
    10321 gcataagatt tgccacctta ctgatttgct ctaatggagc agacatctgt tgataaaggg
    10381 tatcggaagt gctaagttct acatcatttc cgaatatttt ttgcaagctg gtttccagca
    10441 catctaattt ttctggtctt tttgaataaa cgcagagttt ttcaaattca gcttctttga
    10501 acaattcttt ataagtgctg ttgtcaataa agacttgatt ttctattcga ttgactgcgg
    10561 tagtttctgt tggctggtct ttttccacat ttcctgcgga catgaaaatt ccaataattt
    10621 gaacagatac ttttgttccg ttttctgtac ttaattcaat cgtatctcca acttttagct
    10681 cattttgatt tgcaaggttc gcattgataa cagcaccttt tttctcgtgt ttgatataat
    10741 cgccagaaga taagcggtac tgcatttctg aaaaagggct gtccttttcc aaatcatcat
    10801 aggagagcaa cgctattttc aaattttcct cattcgtaga agtattgagc gtaacgggag
    10861 caaaatcatt cggaaatacc tcttgtctgc cgatacggtt gatagacgaa acatcttcca
    10921 gcgtttcaat cttgtttact tcattctctg ttatcttgct gtccttactt gtaatttcag
    10981 ccacaatctt agagtttgtc ttttcctgca tggcttgttt ggactcattt gtagtcctta
    11041 aaatcatgct tgtacctaaa atcatactgt ttacaagtat caaaacaatc aacagaagaa
    11101 tggtttttcc tttttttcgc tgtaaatagc gaaaaccaag ttgataaaac ttcatacttc
    11161 acacctctat tcccaacggg ctatgacaac ctttgttgca ttgaaatgaa gtgtgtctga
    11221 aaattctcca tagagatata catttgattg tttctttaca tcggatatag accccgttgt
    11281 tatattcgta acagaaccca actgtttatt tagtgtcgca acaaaaaatt cacaatctgg
    11341 gttataggtt acagaaacag cattgtcttt attttccaat cccgacgctt cgatttgcag
    11401 gctagctcca ccctcagttt cttttgcttg acttacggaa caaccattgt ctgtaaattc
    11461 caaaacactt cctatcaaat tagcagactc taatatgtcg tttcctttat tttctgaaga
    11521 accatttgca ttttcttcat tcgtattaga attgacaata ggattagacg tatctggttt
    11581 ctcatctgga cttgcaccac accccgtcaa tgcacagata cataatccaa ctgctacaaa
    11641 tttactgatg tttgtttttt tcatttttgt attccacctt tcttgttcga tatgccccta
    11701 ttgtaaaagc aaaccattct aaaaatcctg taaaaaagta agaaaatcat catcattttt
    11761 tagaggattt ttacagactt ttctgttata ctgtctataa tttatataga aaaggattga
    11821 tacattatga aaatacttct tctggaagat gatttagagt tatgtaaaaa tataaaaatt
    11881 tctctcgaaa aagaaaacta tatggttgat tgttgcaatg acggggaaac cgctatgctg
    11941 tacgctttaa atacagacta tgcttatgac ctcgccattg tagaccgtat gctaccgatt
    12001 attgacggct taacaatcat aaaatctatg cgtagaaaag gaatatctat tcctatcctt
    12061 atcattactg ccatgagtac ccttgacaat cgggtggagg gtttagacgg cggtgctgat
    12121 gattacttag taaagccatt tcatatcaaa gaattgcttg caagagttcg ggcattaaca
    12181 agacgccctg ccaatatcca gttatcttcc aaattagaat atggagattt aactttcgat
    12241 aaagacagcc gaaccttatc tacacccgtc aattctcttt tattaacttc aaaagaaacc
    12301 gagttgactt ttgtatttat gcagaaccca gaaacactat tttcccgtga acagctaatc
    12361 cttaaaatct ggggcagttc ttccgaggta gaaactggca atgtagacag ttacatttct
    12421 tttctgcgaa aacgcctaaa agaattaaaa agttcctgta aaataacaac ggtctacggt
    12481 gcagggtaca aactggagaa agaaacatgt taaataactt ataccgaaaa cttcatattc
    12541 tgtttgcaag ttctgttatg ctgattatta cccttgtgat ttcctttgtc gttgcgaaca
    12601 cagttcatac agagaaaatc aacgaaagca ccttatttca gagattgaca accttgctga
    12661 tttatcaagt ggaaagtgct tcttcggata tggacaaagc actcaaaacc tacgaagaga
    12721 gttatcatat tttttcttta attactgata caagggggaa taccatttat cagagtgatt
    12781 tcccatttcc aacttctgcg gacaatctgt tgcatgatgt agaaaagcag atttctacac
    12841 aaccattatc acaaatagaa aacagcacaa cttcacaagg cggttttctg gaaataagag
    12901 gaacacatca cgatacttat tttgttattc ctgctacaat taggacagca aatgataccg
    12961 tgtatcatgc aacctttctt tatcagacag ccagtttgac ggatatttta caaaaaacat
    13021 tgcctatata tcttcttatc tggtttttgg cttgtatcgt tgttatcgtg ctgacccgtt
    13081 atctgctgaa aaagtcgttt gctccaacgg aacgaatact gcaaagccaa aaagattttg
    13141 ttgcaacagc gtcccatgaa ttaaaatctc cacttgctgt tatgatttcc aacacggata
    13201 tgcttcttga taatgtttcc ttaaacgagc aggcaaagca agccgtacag accatagatt
    13261 ttgaatgtat gcggttatcc cgtttagtca aagatatgtt gcttttggct tcttccgacg
    13321 ctaaaacatg gacattgcat aaaagcacaa tcaatgtaga taccttgctc attacgttat
    13381 atgaaaccta cgaacccgtc tgcatgaagc agaatttaga actaaaactg catttatccg
    13441 aggaaagtta tcctgctatg tttaccgaca aagaccgatt atttcaaatc ttgtgtgtct
    13501 ttatggataa tgccattcaa cattccaaga ataattcttt aatagagata gaagtaatcg
    13561 ccaccgaaaa aaatattgct ttttctgtgg tagaccacgg gcaaggcatt tctgatgaag
    13621 ataagcagta tatctttgac cgattttata gtggcgacaa gtcccatacg aacaaatcga
    13681 attttggact gggactaagt atcgccaaag aattaactca aatgctgaac ggtattatca
    13741 ttatcaatga tactgctggt ggaggtgcta cttttactgt taaatttcct ctgaaataga
    13801 ttaaggcttg cagatcatac actatgtatg gagcaagcct tttttcaaat ctttgtcttt
    13861 ttggtaatct tgtaaatata ttcttctggg gtagggcgtt tattaccaaa atactttttg
    13921 aaatttgcct gcacttctgg ggcggtgcta ttcatagctt catcaatcgt cgcttcaata
    13981 tctgcccgaa catcagccaa tgatacaccg ccagcttttg caccagcttt caacgctttt
    14041 tttatatctc gcttttttag tcctatcata ataacctttc ttatcataat tgcaaattta
    14101 taaattcatt agtattaaaa ctgaaattca aatacattac taaaatcatt gttcaagaca
    14161 taataaactt tatttttttc tgtagaatat acaaatgaaa ccctagttgc ccacaagtct
    14221 ttttgagaaa cagattttaa tactttaaat gcgtcattta ctgaaaaatc tttatcatat
    14281 cctgccaata actctttagc cacttcgtat cttgtatccc cagaaacaat gtacggcttt
    14341 gttatatctg gttttagaat agaataattt gtaataagtg aaaaattttc ttgttcatat
    14401 ctatacccaa ttccaggttc aataattaaa actcgtcctt ttttatcaga aagcatagct
    14461 tgcatagtag catctggtgc ataaataatt tttttctttt taacaatgtc caaggcttta
    14521 tcaaaagaaa tctctgcctt aatgaatttt tctgttaatt cagatattgt aatacaatcc
    14581 tcgctttcta tataccttgc attttcatta ccatgaacat aaagaagtgt tcctacatta
    14641 ccatttttat ttatcccatg aaatgaatga tacagaccgt caggcatttt aataccaata
    14701 taaaatctat tttcttcggt aattacagta tgatcccata cagaaaggtc aatatcaaaa
    14761 ttaaaaccaa caattttatc tataccattg taaacaaatc ttgtacacat ataatcactc
    14821 ctcaaaatac tattttaaat agtcatttaa ctaatctaat tagtatttta aaatgttttt
    14881 gtaagaaagt caataaataa tcataatttt ctaattgtgt tattgactac actaattcaa
    14941 ttagctagaa ttgtgtttgt atgatatttt gaatgaggtg cataactatg aaaaccacta
    15001 aagaagcagt aataaaagca acatcagact tagcagatga aaaaggatta aataacgttt
    15061 cgttaaaagc tgttgctcaa aaattaaaca ttcgtacacc atctttatat aatcacatag
    15121 aaagtttaga taatcttctt ttagaagttg ctcataatgg tatgcgtgac atgaatgaaa
    15181 gaatgatgaa aattgccgtt ggaaaaatag ggaaagaagc aattaagtta gtttctatcg
    15241 aatacctaaa ctatatgatt gaacaccccg gagtttatga aacaattcaa tgggctgtgt
    15301 ggcacggaac agaggaaaca gcaaccattt ttaacaacta tctttcatta ttaactactc
    15361 ttatccaatc gtgcagtctt aacaaagaca aaacacttga aatattaaat atgcttactg
    15421 gaataattca cggttataca actttgcaat taggaaacgc tttttctgcc tcagataaag
    15481 ttagatttga attagctgaa gcgatagata ctttattagt cgggattttc cagaagtata
    15541 aataatatat ttaggcttgc aaatcaatac agaactgcaa gcctttttct tttatcctac
    15601 aatcttccag ttgctatcct tatgtagcac aagctcatac tgtgatacct gcgtcgcctt
    15661 tgtctgatta tcaaggaatt ttaccgctac cttgactttc acattatccc cgttctttgt
    15721 aaagataggg tttaccagtt cagaataaag gtagtccctg ccgataggct caatcacatt
    15781 tccagataca tagtaagcaa gctctttttc tgtggctgtt ggatagagct taaagaatgt
    15841 ttccagaaat gcggtagcgt catttaccgt atcagcgtcc acgcttgcgt ctgcttctgg
    15901 tgtcttaggc tcatagtctg atttttcgat tgctggtgca agagtagggt tctgaatgat
    15961 taccatatcc ccgtcagcgt ctacatggac ttttacagta taagtcgctt tcacattgct
    16021 tgtctgttct ccctctttta tctgctgatc tacttcgtag gtagcagaaa aagtgtccgt
    16081 tcccgattgc tcgatatgcc acacaatcac atctgtaact gtggagctgg tcggtatatc
    16141 cgttctgatt gtatcaacat tcaagtcttg caattccttt gtcagataac cgctgattgc
    16201 ctgcgtcctt gcttcaatcg cttctttgct gttactccat gtgtaataag acttcgcaaa
    16261 gttcttcacg aaattttcta tcccgttggt gtcctgcaaa cgaagttcaa tgatttcttt
    16321 ttcatgggtc gtgtgctggt cgatagccgt aaaattctta tacaccccaa agctcacgct
    16381 tgcgataagc accacccaca acgcaatcat ggttttctta tgtgtgccta ccttgacagt
    16441 acgcaccttt ttttcttttg gttctttgac agtttctgtc tgtttcttat ttttcttaaa
    16501 catattttca agtcctttct attgttttac tcgtcctgca ccgattaagt gctgttgcca
    16561 gtaactacta cttaggtctg catatcctat cgggtcgcct gcatgataca tctgattatt
    16621 tcc
//
LOCUS       DBGJFU010000087        16350 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.087, whole genome shotgun sequence.
ACCESSION   DBGJFU010000087 DBGJFU010000000
VERSION     DBGJFU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.087"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..369
                     /gene="tuf"
                     /locus_tag="ACOIAS_002599"
     CDS             <1..369
                     /gene="tuf"
                     /locus_tag="ACOIAS_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791980.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGZ2702302.1"
                     /translation="DNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEE
                     GGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLR
                     FSIREGGRTVASGVVATIIE"
     gene            619..768
                     /gene="rpmG"
                     /locus_tag="ACOIAS_002600"
     CDS             619..768
                     /gene="rpmG"
                     /locus_tag="ACOIAS_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGZ2702303.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            794..1015
                     /gene="secE"
                     /locus_tag="ACOIAS_002601"
     CDS             794..1015
                     /gene="secE"
                     /locus_tag="ACOIAS_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGZ2702304.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1035..1577
                     /gene="nusG"
                     /locus_tag="ACOIAS_002602"
     CDS             1035..1577
                     /gene="nusG"
                     /locus_tag="ACOIAS_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGZ2702305.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1616..2041
                     /gene="rplK"
                     /locus_tag="ACOIAS_002603"
     CDS             1616..2041
                     /gene="rplK"
                     /locus_tag="ACOIAS_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGZ2702306.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2112..2810
                     /gene="rplA"
                     /locus_tag="ACOIAS_002604"
     CDS             2112..2810
                     /gene="rplA"
                     /locus_tag="ACOIAS_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGZ2702307.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2862..2990
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3033..3539
                     /gene="rplJ"
                     /locus_tag="ACOIAS_002605"
     CDS             3033..3539
                     /gene="rplJ"
                     /locus_tag="ACOIAS_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGZ2702308.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3597..3962
                     /gene="rplL"
                     /locus_tag="ACOIAS_002606"
     CDS             3597..3962
                     /gene="rplL"
                     /locus_tag="ACOIAS_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGZ2702309.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4354..5142
                     /locus_tag="ACOIAS_002607"
     CDS             4354..5142
                     /locus_tag="ACOIAS_002607"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGZ2702310.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5613..9329
                     /locus_tag="ACOIAS_002608"
     CDS             5613..9329
                     /locus_tag="ACOIAS_002608"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGZ2702311.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIVKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9371..12856
                     /gene="rpoC"
                     /locus_tag="ACOIAS_002609"
     CDS             9371..12856
                     /gene="rpoC"
                     /locus_tag="ACOIAS_002609"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGZ2702312.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13145..13567
                     /gene="rpsL"
                     /locus_tag="ACOIAS_002610"
     CDS             13145..13567
                     /gene="rpsL"
                     /locus_tag="ACOIAS_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGZ2702313.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13691..14161
                     /gene="rpsG"
                     /locus_tag="ACOIAS_002611"
     CDS             13691..14161
                     /gene="rpsG"
                     /locus_tag="ACOIAS_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGZ2702314.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14210..16276
                     /gene="fusA"
                     /locus_tag="ACOIAS_002612"
     CDS             14210..16276
                     /gene="fusA"
                     /locus_tag="ACOIAS_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGZ2702315.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6411 a         2033 c         3339 g         4567 t
ORIGIN      
        1 gataatatag gagcattatt aagaggagta caaagaaacg agatagaaag aggacaagta
       61 ctagcaaaga ctggatcagt aaaggcacac acaaagttta cagcagaagt atatgtactt
      121 aaaaaagaag agggtggaag acatacacca ttctttgatg gatatagacc acaattctat
      181 ttcagaacaa cagacgtaac aggagcttgt aagttaccag aaggaataga gatggtaatg
      241 cctggagata acgtaacaat ggaagtagac ttaataaact caatagttgt agaagaggga
      301 ttaagattct caataagaga aggtggaaga acagtagctt caggagttgt tgctacaata
      361 atagagtaat tctttactaa actgatggat taaaaggaat aacaaaaaga ggggttatgc
      421 tcctcttttt ttaacataga aatacaaaaa cattgacaca gcataggatt ttgtgataac
      481 ttatacaagg taatgattat aggaagttaa taatatcttg aatactgtag atttttaata
      541 aatacatatt ttaaagatat tatattcaca tatattaaca atatatatta ttgtattaca
      601 aagttggagg tgtatcagat gagagttaaa gtaactttag catgtacaga gtgtaagcaa
      661 agaaactaca acactactaa gaataagaaa aataatccag acagaataga attacaaaaa
      721 tattgtagat tctgtaaaaa gcatacaact cataaagaaa caaaatagtt ttttataagg
      781 atgtgagtat ggaatggctg cccaaaaaaa tgacggtgca aaaactaaaa aaaggtttag
      841 tttgtttgga tacttaaaag aaactaaaca agaattaaaa agagttacat ggccgacaaa
      901 aaaggaactg tttaaaaaca caagtatagt tttaactgta gttatttcat gcactatatt
      961 agtatggggt atagacacta tattatctgg tgcactagcg ttattactaa aatagacgaa
     1021 aagaggtaag ttaaatgtca gaattacaag aagcaagttg gtatgtagtt catacctatt
     1081 cgggacatga aaataaagtt aaagctacaa ttgagaaagc agttaaaaca agaggtatgg
     1141 aagactgcat aagacaagta gttgttccaa cagaagaagt agtggaaact actaaaactg
     1201 gaaaagaaaa aactagacaa cgtaaagttt atccaagtta tgttttggtt aaaatgataa
     1261 taactgatga atcttggtat gtagtaagaa atactaaggg agttacaggt ttcgttggac
     1321 ctggttcaaa accagtgcca ttaagtgaag acgaagtcaa agctatgggg atagacacaa
     1381 cagaccctaa agtagtaaat agcgatattg attttgaaat aggagataca gtaaaagtat
     1441 ctcaaggacc atttagtgga caaataggta acatagaaga aattgactta gaaaatagag
     1501 aagtcaaagt atgcataaat gcatttggta aaaggactct atttgtaata gagcttgaag
     1561 gtatagaaaa aatctaatac tatattatag aaaaatctga ggaggtgcgc tcaaaatggc
     1621 taaaaaagtt ataggtcaaa taaaattaca aatacctgca ggaaaggcta ctccagcgcc
     1681 accagttgga ccagcattag gacaacatgg tgttaatata atgggattca caaaagaatt
     1741 taatgctaaa actgcagatc aagctggaat gataatacca gttgttataa ctgtatatca
     1801 agatagatca ttcagtttta taacaaaaac tccaccagct gcagttttaa ttaaaaaagc
     1861 attaaactta aaatcaggtt ctggagaacc aaacaagaaa aaagttgcta aaatgacttc
     1921 agctcaagta agagaaatag ctgaattaaa gatgcctgac ttaaatgctg catcagtaga
     1981 agctgctatg agtatgatag ctggtactgc tagaagcatg ggtgttgtaa tcgaagacta
     2041 gttaaaagat acactttttt ttataaagtg ggaggctaga agccgaaatt aaccacgagg
     2101 aggaaaataa aatggctaaa aaaggtaaaa gatatgcagg tgcattacaa aaagtagata
     2161 gaactaaatt ttatgatgca tcagaagcat taacattagt ttcagatata gctggagcta
     2221 aatttgatga gacagtagaa gcacacatta aattaggtgt tgactcaaga catgctgacc
     2281 aacaagtaag aggtgcagtt gtattacctc atggaactgg taaaacaaaa agagttttag
     2341 tttttgctaa aggtgaaaaa gctaaagaag ctgaacaagc tggtgctgac tttgtaggag
     2401 ctgaagaatt agttcaaaaa atacaaggtg aaaactggtt tgactttgat atagtagttg
     2461 ctactccaga tatgatgggt gtagtaggta gattaggtag agtattagga cctaaaggtt
     2521 taatgccaaa ccctaaatca ggaacagtta catttgatgt agctaaagct atagatgaaa
     2581 taaaagctgg taaagttgaa tacagattag acaaaactaa cataatacac gttccagtag
     2641 gaaaagtatc atttggtgga gaaaaattaa ctgaaaactt tactgcatta atggatgcta
     2701 taataaaagc taaaccagct gctgctaaag gacaatattt aagaagtata acagtagctt
     2761 cttcaatggg acctggagtt aaaataaacc cagcaagaac agctgaataa taaaactgtt
     2821 gacacaggtt gacaaaaatg ttattatatc acttgtcaat gtaaaaaaga atagatttgc
     2881 cgtagatagt gggtgcgaaa gcttaatatc ccgccgaggt tttagataaa ttgagattac
     2941 taaagctttt gatgtctact tttggcaaca aaagcttttt ataatgttgc ggtaaatact
     3001 aagcctattt ggtgaaggag gtgcacatct atatgagaaa agctatagaa attaaatcag
     3061 aagttgtttc tgagatagtt gaaaagttac aaaaatcttc tgctgctgtt gttgtagatt
     3121 acaaaggatt aacagttgaa gaagtaactg agttaagaaa acaaatgaga gaagctggtg
     3181 tagattacaa agtatacaaa aatactttag ttagaagagc agctaaagaa gttggtatag
     3241 agcaatttaa tgatgaatta ttagttggaa ctaatgccat agcatttgga tatgatgatc
     3301 cagttgctcc agctagaata ttaaaaggat tcatggattc tcatccaaaa atgaaattaa
     3361 agatgggtat tgtggaaggt gcattctatg atgaatctaa gattgttgaa atggctaaca
     3421 taccatcaag agaagttctt atcgctaaat tacttggtag cttaaaagct ccagtatcaa
     3481 acttcgcata cttaatagat gcaatagcga aaaaggcaga aggtcaagaa gaagcttaat
     3541 tgcttaacta aaaaattata aatattaatt tgaaataaaa ttcggaggtg ctaaaaatga
     3601 caatagaaca aatattagaa gctatagaaa atatgaaagt tttagaatta aatgaattag
     3661 ttaaagctgc tgaagaaaaa ttcggtgtat cagcttcagc tccagtaatg gtagctggtg
     3721 cagctgctgg tggaccagct gctgaagaaa aaactgagtt tgatgtagta ttaactgacg
     3781 ttggttcttc aaaagttgga gttataaaag cagttagaga aataacagga ttaggattaa
     3841 aagaagctaa agaagtagtt gacaatgctc ctaagacagt taaagaagga gcttctaaag
     3901 aagaagctga tcaaataaaa gagaaattag aagctgctgg agctaaagta gaagttaagt
     3961 agtttcttta aaaagatacc cattgggtat ctttttttat ttataaaaaa ctataaagaa
     4021 agtatttgga attataaaga aagtattttt ataaaataaa aaaaatttat ataatagtac
     4081 taaattaagg gtatcatata tggtgtaatg gtattttaat ttaacattat tatcaaatta
     4141 aaatataaac aattgttgta atgtctatat ataaagtata taattaaact aaaatgtaaa
     4201 attttacatt ttagtttaaa aaaatacacg aatgttttat aaataaaatg taataaataa
     4261 ttaattaaca aatataaatt ttaaaataag ttagatattt agaaaaatcg aaatttgtag
     4321 gatttaaagg acatattcaa ggaggagtta ataatggaaa aattacaagg aaaaattgca
     4381 gtagttactg cagcaacaaa aggtattgga ttagcatcag cagagatatt agcaaaaaat
     4441 ggagcaactg tgtacttagc agctcgttca gaagaattag ctaatgaagt tataaataaa
     4501 ataagtgcag aaggtggttg tgctaagttt gtttacttta atgctcgtga agaagagacc
     4561 tttacctcaa tgatagaaga ggtagttaaa aaagaaggta agatagatat acttgtaaac
     4621 aattttggtt caacaaaccc ttctcttgac aaagaccttg taactgggga tacagataat
     4681 ttctttgaca cagtaaatac taatttaaaa agtgtgtatt taccatgtaa agcagcaatt
     4741 cctcatatga taaagaatgg aaaaggaagt atagtaaata tatcaagtat aggttcagta
     4801 ttacctgatt tatctagaat agcttactgt gtatcaaaag cagcaatcaa ctcattaact
     4861 caaaatatag ctacacaata tgctaaagac aatgttagat gtaatgctgt acttccaggg
     4921 cttattgcaa ctaaagcagc attagacaat atgtctccag agttcataaa agaattttta
     4981 aagcatgttc ctttaaatcg tataggggaa ccagatgata tagcaaaagc tgttctattc
     5041 tatgctagtg atgactcatc atttataaca ggggatttac ttgaagttgc aggaggattt
     5101 ggtttaccta ctccacaatt tgcagataat atattaggat aattaaatta ctaatatgca
     5161 atgaaagctt atgcattagt tatagtagaa atgatatttt tataaaggcc atagatataa
     5221 tttatattgg aaaggtttga ggttcaaaat tccaagttaa atttatttat ggtctttatt
     5281 ttgtatattt taataaattg tatatatttg tataaatgta attatttaca atcattttaa
     5341 tatggaatta taattaaata ggggggatgg tacttgtata aaatttttta tagaaaatta
     5401 gacctataaa tgaaaaatat ggattttata ttggaaaatt gtacataata cataatatat
     5461 gcttaaaata atttacaaaa aatcaaaaat aatgcatagt ttacatataa gataaaaaat
     5521 atattgaaaa atacttgaca ttatgctaaa attattagat gcgttagaat gaactattgt
     5581 tttattttta attattgaga ggtgaaaagt gaatgccaca tcctgtcacg ataggtaaaa
     5641 gaactagaat gagcttttct aaaattaagg aaatagctga tgtgccaaat cttatagaaa
     5701 ttcaagtaga ttcctatgag tggtttttaa aagaaggttt aaaagaagtg tttgatgata
     5761 tttcaccaat agaagattat actggtaatc ttatattgga atttgtcgac tattctctag
     5821 atgataaacc aaagtacgat atagaagagt gtaaagagag agatgctaca tattgtgcac
     5881 ctctaaaggt taaggtaaga cttataaata aagaaacagg tgagataaaa gaacaagaag
     5941 tgtttatggg tgacttccct ttaatgacag aaagaggaac tttcgttata aatggagcag
     6001 aaagagttat agtaagtcaa ttagttagat ctcctggtgt ttactatgcg gaagaaagag
     6061 ataaaacagg taagagattg atttcatcta ctgtaattcc taatagagga gcatggttag
     6121 aatatgaaac agattctaat gatgtaatat ctgtaagagt tgatagaaca agaaaacaac
     6181 cagttacagt tttacttaga gctttaggaa taggaacaga tgcagagata attgaccttt
     6241 taggagaaga tgagagatta tctgctacat tagaaaaaga taatactaag acagtagaag
     6301 aaggtcttgt agagatatac aaaaaattaa gaccaggtga acctcctact gtagaaagtg
     6361 catcatcttt attaaatgct ttattctttg acccaaaaag atatgaccta gcaaaagttg
     6421 gtagatataa attcaataag aaacttgctt tatgttacag aataatgaat aaaatttcag
     6481 cagaagacat aataaatcca gaaactggag aagtatttgt aaaagctgga gaaaaaataa
     6541 gctatgattt ggctaaagcc atacaaaatg caggtataaa tgtagtaaat ctactaatgg
     6601 atgatgataa aaaagttaga gttataggaa ataattttgt ggatattaag tctcacattg
     6661 attttgacat agatgattta aacataaaag aaaaagtaca ttatccaact ttaaaagaaa
     6721 ttttagatgg atatagtgat gaagaagaaa taaaagaggc tataaaatca agaattaaag
     6781 aacttatacc aaaacatata ttattagatg atataatagc ttcaataagt tatgagttca
     6841 atatattcta taatatagga aatattgatg atatagatca cctaggaaac agaagaataa
     6901 gatctgtagg tgaattgtta caaaatcaag ttagaattgg tctttcaaga atggaaagag
     6961 ttataaaaga aagaatgacg gttcaagata tggaagctat aacgcctcaa gcgttagtta
     7021 acataagacc agtttcagct gcaataaaag aattttttgg tagttctcag ttatctcagt
     7081 tcatggatca aacaaatcct ctatctgagt taacgcataa gagaagatta tcagcccttg
     7141 ggcctggagg tctttcaaga gaaagagctg gattcgaagt gcgtgacgtt caccattcac
     7201 actatggtag aatgtgtcca atagagactc cagagggacc aaatatcggt cttataaact
     7261 ctctaggaac ttatgcaaaa ataaatgaat ttggatttat agaatcacca tatagaaaat
     7321 ttgataaaga aacatcaaca gttactgatg aaatacatta tttaactgct gacgaagaag
     7381 atttattcgt aagagctcag gcaaatgagc cattaacaga agatggtaaa ttcgtaaacc
     7441 atagagttgt ttgtagaact gtaaatggtg ctgttgaaat ggttcctgaa agtagagtag
     7501 attacatgga tatatctcct aagcaggttg tatctgttgc cactgctatg atacctttct
     7561 tagaaaatga tgatgccaac cgtgccctta tgggagcgaa catgcagcgt caggcagtgc
     7621 ctctagttag aagagaagca ccaattatag gaactggtat agaatacaga gcagcaaaag
     7681 actctggagc agttgttgtt gctagaaatt ctggtatagc agaaagagta acagcagatg
     7741 aaataattat aaaaagagaa gatggaaaca gagatagata taatctactt aagtttaaac
     7801 gttcaaactc aggtacttgt ataaatcaaa cacctataat aaataagggt gaccagatag
     7861 taaaaggtga tgttatagca gatggtccag caactgattt aggagaagtt gcacttggaa
     7921 gaaactgtct tatagcattc atgacttggg aaggttataa ctacgaggat gctatattaa
     7981 taaatgaaag attagttaaa gaagatagat tatcaacaat tcatatagaa gaatatgaat
     8041 gtgaagctag agatacaaaa ctagggccag aggaaataac tagagatata cctaatgttg
     8101 gagatagtgc aattaagaac ttagatgaca gaggtataat aagaataggt gcagaagtag
     8161 actcaggtga tatattagtt ggtaaagtaa ctccaaaagg agaaactgaa cttactgctg
     8221 aggaaagatt acttcgtgca atatttggag aaaaggctag agaagttaga gatacttcac
     8281 ttaaagtgcc tcatggtgaa tctggtataa tagttgatgt aaaagtattt actagagaaa
     8341 atggagatga tttatctcca ggagtaaatg aattagtaag atgctatata gctaaaaaga
     8401 gaaaaataaa agttggagat aaaatggctg gtcgtcatgg taataaaggg gttatctcaa
     8461 gagtattacc tgaagaagat atgccattca tggaaaatgg aacaccacta gacataatac
     8521 ttaacccaca aggtatacca tcacgtatga acattggtca ggttttagaa gttcatttag
     8581 gacttgctgc taagacatta ggatggtatg tagcaacatc agtattcgat ggtgctaatg
     8641 agtatgacat aatggatgct cttgaagaag ctgggtatcc tagagatggt aaattaactt
     8701 tatatgatgg tagaacaggt gagtcttttg acaacagaat aactgttgga tatatgtact
     8761 acttgaaact acatcactta gtagatgaaa aactacatgc aagaagtact ggaccatact
     8821 cattagttac tcaacagcca ctaggtggta aagcacaatt tggtggacaa agatttggag
     8881 agatggaggt ttgggctctg gaagcatatg gagctgctca catacttcaa gaaatactta
     8941 ctgttaagtc tgatgacgtt gtaggtcgtg ttagaacata tgaagctata gttaaaggtg
     9001 aaaatatacc tgaaccagga atcccagaat catttaaggt tcttataaaa gaacttcaaa
     9061 gtttatgctt agatgtaaaa gtattgacag atgaagacca agaaatagaa gtaagagaat
     9121 ctgtggatga agatgataca ataggtgaat ttgagctaga tgttgtaaat catatgggag
     9181 aagttgaaga aagtaatata atagaagaaa ttgaagatga ttttgcagaa aatgcagaag
     9241 atgaggatat agagaattta gaagaattca ctgaagatga tttatttgaa gaagaaatag
     9301 attttgatag tgatgacttt gatatgtaaa tttaattaat gtaaatctca aatagaaggg
     9361 agagaactcc ttgtttgaat taaacaattt cgagtcgata aaaatagcat tggcttctcc
     9421 agaaaaaata agacaatggt ctaggggaga agttaaaaag ccagaaacta taaattaccg
     9481 tactttaaaa ccagaaaaag atggtctttt ctgtgaaaga atatttggac cacaaaaaga
     9541 ctgggagtgc cactgtggta aatatagaag agttagatat aaaggtgtag tttgtgatag
     9601 atgtggagta gaagtaacta aatcaaaagt aagaagagag agaatgggac atatagagct
     9661 agcggctcct atgtctcaca tctggtactt caaaggtata ccaagtagaa tgggactttt
     9721 acttgatatg tcaccaagat cgttagaaaa aatattatac tttgcctcat atgtggtagt
     9781 tgatccagga gagactggat taaatgaaaa acaattgctt acagaaaaag aatacagaac
     9841 tgctcttgaa aagtatggat atacttttac tgtaggaatg ggtgctgaag ctgtaaagac
     9901 attactacaa aatatagatt tagagcaaca aagtaaagac ctaagagcgg agttaaaaga
     9961 tagtacaggg caaaagaaag ttagaacaat aagaagatta gaagttgtag aggcatttaa
    10021 aaagtctgga aataaaccag aatggatgat tttagatgca ataccagtaa taccaccaga
    10081 tttaagacca atggtacaac ttgatggtgg aagatttgcg acttcagacc taaatgattt
    10141 atatagaaga gttataaata gaaacaatag acttaaaaga ttattagagc ttggagctcc
    10201 agatataatt gtaagaaatg aaaaaagaat gcttcaagaa gctgttgatg cattgataga
    10261 taatggtaga agaggtagac ctgtaacagg acctggaaat agaccactta aatctttatc
    10321 agatatgtta aaaggtaagc aaggtcgttt ccgtcaaaac ttacttggta agcgtgttga
    10381 ctactcagga cgttctgtta tagttgttgg accagaactt aaattttatc aatgtggact
    10441 tccaaagaag atggcattag aattattcaa gccatttgtt atggataaat tagttaaaga
    10501 aggttatgca cataatataa aaagtgcgaa atctatagta gaaaaagtta agccagaagt
    10561 ttgggatgtt ttagaagatg ttataaaaag tcatccagtt cttcttaacc gtgcaccgac
    10621 tctacataga ttaggtatac aagcatttga accaatctta gttgaaggta aggctataaa
    10681 actacatcct cttgtatgta cagcttataa tgcagacttt gatggtgacc aaatggcagt
    10741 tcatgtacct ttatcagtag aagcacaagc agaagcaaga ttcttaatgc tttctgtaaa
    10801 taatatactt gctcctaaag atggttcacc tataactact ccatctcagg atatggtttt
    10861 aggttgctat tatctaacaa tagaagccca agatggagct aaaggaacag gtatggtatt
    10921 taaagacttt aatgaactat tacttgctta ttacaataaa tcagttcatc tacatgcatt
    10981 agtgaaactt aaggtaacac ttgaagatgg aagaagttca ttggttgaaa gtactgttgg
    11041 tagatttata tttaatgaaa atatacctca agacttaggt tttgtagata gaaaagaaaa
    11101 tccatttgca cttgaagttg atttcttagc agataaaaaa tctcttggta aaataataga
    11161 taaatgcttt agaaaacacg gaaatacaga aacagctgaa ttactagatt atataaaagc
    11221 tctaggattt aaatattcta cattaggtgg cataacagtt gctgttgatg atatgagtgt
    11281 cccagaagaa aagaaagtat ttatagctga ggcagaagct aaagttgata agtatgaaaa
    11341 agcatacaga agaggtctaa tctctgatga agaaagatat gaaaaagtta tagagacatg
    11401 gacagaaaca actgataaag ttactgatgc tcttatgggt ggactagata gattaaataa
    11461 tatatatata atggcacatt caggagccag aggttctaaa aaccaaatta gacagctggc
    11521 aggtatgcgt ggtcttatgg caaatgcatc tggtaaaaca gttgagatac cagttaaatc
    11581 taatttccgt gaaggtttat cagtactaga atactttaca tcttcacatg gtgccagaaa
    11641 aggtcttgcc gatacagcta tacgtacagc tgaatctgga tatttaacaa gaagacttgt
    11701 tgatgtaagt caagatgtta ttgtaaggga aatagattgt ggaacagaag atactacaga
    11761 gatttatgct ataaaagaag gaaatgaagt tatagaagag atatatgata gaattgtagg
    11821 aagatatact atagacccta tattaaatcc tgaaactgga gaagttatag ttgaggctga
    11881 ttcaatgata caagaagatg aagcagaaac tatagtagct ttaggaattg aaaaaattag
    11941 gataagaaca gttcttaact gtaaaactaa tcatggagtt tgttctaagt gttatggtag
    12001 aaacttggca acaggaaaag aagttaatat aggtgaagca gttggtataa tagcagctca
    12061 atctatcggt gagccgggta ctcagcttac aatgcgtaca ttccatactg gaggagttgc
    12121 aggagctgat ataactcaag gtctaccaag ggttgaagaa ttatttgaag ctagaaaacc
    12181 aaaaggatta gctgtaataa ctgaagtatc tggtagagtt gaaattgatg aaacaggaaa
    12241 gagaaaagaa gtaaatgtaa taccagaaga aggcgagact caaacatatg taattccata
    12301 tggttctaga ttaaaagtta agcaaggtca aatgctagag gctggagacc ctctaacaca
    12361 aggatttata aatcctcatg atatagtaag agtaaatggt gttaagggag ttcaagaata
    12421 tatagttaaa gaagttcaaa gagtgtatag acttcaaggg gttgatgtta atgataagca
    12481 tatagaagtt atagtaagac aaatgctatc taaagtaaaa gttgaagacc caggagatac
    12541 agatttattg ccaggtggat atgaagatgt attaacattc aatgaatgta ataaagatgc
    12601 tatagataaa ggtctaagac cagcagttgc taaaagagtt ttacttggta taactaaagc
    12661 atctcttgca actgattcat tcttatcagc agcttctttc caagaaacaa caagagtatt
    12721 aacagaggca gcaataaaag gtaaagaaga ccacttaata ggacttaaag aaaatgttat
    12781 attaggtaag ttaataccag caggaacagg aatgaagaaa tatagaaata tagctgttga
    12841 aaaaattgaa gattaatagc atatagagta aagaaatctt gacaaccatt atttgtggtg
    12901 ttaaaatagt aaagtgtgtt gtttaaaagt acactaaggg cttgaacaag ttcttagtga
    12961 cttttagatg gcaataaatg agaactaaaa agcatcaagc tagaaaagtg ctaatatcac
    13021 atatttatgt gttatagaca ttaaattgta agtaacgaat gttacaatca gtcagtatga
    13081 atattcatat tgatgtagtt ggcggtcact gataagatcg aaaatttgga aggaggtgca
    13141 gatgatgcca acaattaacc aattagttcg taaaagtaga aaagcgctag agaagaaatc
    13201 tactgctcca gcattacaaa aaggttacaa ctctttaaat aagaaagtaa ctgacgctag
    13261 tgccccacaa aaaagagggg tttgtacttc agtaaaaaca gttactccta agaagcctaa
    13321 ctcagcttta agaaaagttg ccagagttag attaactaat ggtatagaag tttctgctta
    13381 tataccagga gaaggacaca acttacaaga gcatagtgtt gttcttataa gaggaggaag
    13441 agtaaaagac cttccagggg ttagatacca tatactaaga ggtactctag atacagcagg
    13501 tgttgataag agaagacaat caagatctaa atacggtgcg aagagaccta aagaagctaa
    13561 aaagtaaaaa caattaatga ttgattatag ttcggtgctt taaatatata tatatttacg
    13621 gcatcacggc attaaatgat ttatgtcgag tacctatgat ttaagttaaa taattaagga
    13681 gggaagcgaa atgccaagaa aaggtaatat tccaaaaaga gaagttttac cagatcctat
    13741 gtatgggagt aaggtagtta ctaaattaat aaataatcta atggtagacg gaaaaaaagg
    13801 aaagtctcaa agaatagttt atgatgcttt cgcaattgtt gctgaaaaga caggagaaga
    13861 agctttagaa gtatttaata aagcaatgga taatataatg cctgttttag aagtaaaagc
    13921 aagaagagtt ggtggggcaa actaccaagt gccaatcgaa gttagacctg aaagaagaca
    13981 aacattaggt ttaagatggc tagtaaaata tactagagct cgtggagaaa aaggaatggt
    14041 tgaaaagcta gctaaagaga taatggacgc agctaacaac actggagctt cagttaagaa
    14101 aaaagaagat actcataaaa tggcagaagc taataaagca tttgctcatt acagatggta
    14161 ggatatatag atatcttata aaattgagga tatcataagg aggataacca tggctagaaa
    14221 gtttcctttg gaaagaacta ggaatatagg aatcatggct catatagatg caggaaaaac
    14281 tactactaca gaaagaatcc tgttttatac tgggcaaact cataaaatag gagaaactca
    14341 tgaaggagct tctcaaatgg actggatgga gcaagaaaag gaaagaggta taacaataac
    14401 ttctgccgct actactgctt catggaaaga ccatagaata aacataatag atactcctgg
    14461 acacgtggac ttcacagttg aggttgaaag atctctaaga gttcttgacg gttcagttgc
    14521 tgtattctgt gcaaaaggtg gggttgagcc tcaatcagaa aatgtatgga gacaagctga
    14581 aacttatgga gtacctagaa tagcttttgt aaataaaatg gatatattag gtgcagattt
    14641 ctacaatgtt gtaagcatga tgaaatctag attaaattct aatgcagttc ctatgcaatt
    14701 accaataggt aaagaagatt catttatagg tataatagac ttattaaaaa tggatgctgt
    14761 tatatacaaa gacgacctag gggttgaaat ggaagaaaca gatataccag aagatatgaa
    14821 agaattagct gctgagtgga gagaaaaatt agttgaatca gtagctgaaa ctgatgaaga
    14881 attaatgatg aaataccttg aaggtgaaga acttactata gatgaattaa aagttgctat
    14941 aagaaaagct actatagctt gtgaaatgaa cccagttttc tgtggtacag cttatagaaa
    15001 caaaggtgtt cagttagtaa tagatgcagt tttagattac ttaccagcgc caactgatat
    15061 accagctata aaaggtatat tagcagatgg agaagaagct gaaagacatt cttctgatga
    15121 agagccattc tctgcattag cattcaaaat aatgactgac ccattcgttg gaaaactagc
    15181 gttcttcaga gtttattctg gaacattaga atcaggttct tatgttttaa atgcaactaa
    15241 gaataagaga gaaagaattg gtcgtatatt acaaatgcat gctaatacaa gagaagaaat
    15301 aactaaagtg tatgctggag atatagcagc agcagtagga ttaaaagata ctactactgg
    15361 agacacatta tgtgaccctg ctaatccaat aatacttgaa tctatggaat tccctgagcc
    15421 agttatatct gttgctatag aaccaagttc taaagcagct caagaaaaaa tgggtatagc
    15481 tcttcaaaaa ttagctgaag aagacccaac tttcacagtt aagactgacc aagaaacagg
    15541 acaaactata atatctggta tgggtgaatt acacttagag ataatagtag atagattatt
    15601 aagagaattt aaagtagaag ctaaagttgg tgcaccacaa gttgcatata gagagactat
    15661 aactcaacca gttgatgttg aatacaagta ctctaagcaa tcaggtggta gaggacaata
    15721 cggtcacgtt aagattagag ttgctcctca agagccaggt gaaggataca aattcacaaa
    15781 caaaactgtt ggtggatcag taccaaaaga atatgttggt ccagttgaca tgggtataca
    15841 aggagctatg caatctggta tagttgctgg atatccagtt gtagacgttg ctgttgagtt
    15901 atatgatggt tcttaccatg aggttgactc ttctgagatg gcgtttaaga tggctggttc
    15961 aatggcattc aaagatgcta tgaagaaagg taatgcagtt cttttagagc cttacttcaa
    16021 agtagaagtt gttactccag aagattacat gggagatgtt atgggtgact taaactcaag
    16081 aagaggatta atccaaggta tggaagctag aagtggtgca caagttataa atgcattcgt
    16141 tccactttca gaaatgtttg gatactcaac agacctaaga tcttcaactc aaggtcgtgc
    16201 aacttacaca atgatattcg atcactatga gcaagttcca gcttcagttg ctaagaaaat
    16261 agctgaagga aaataattta aaaataatta ataatataaa tataaaaaat aatcagctag
    16321 atgagagata tctctcatct agcacaagat
//
LOCUS       DBGJFU010000088        16249 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.088, whole genome shotgun sequence.
ACCESSION   DBGJFU010000088 DBGJFU010000000
VERSION     DBGJFU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.088"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..99
                     /locus_tag="ACOIAS_002613"
     CDS             <1..99
                     /locus_tag="ACOIAS_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2702316.1"
                     /translation="ICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            223..1737
                     /gene="ade"
                     /locus_tag="ACOIAS_002614"
                     /pseudo
     CDS             223..1737
                     /gene="ade"
                     /locus_tag="ACOIAS_002614"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            2089..2553
                     /gene="bcp"
                     /locus_tag="ACOIAS_002615"
     CDS             2089..2553
                     /gene="bcp"
                     /locus_tag="ACOIAS_002615"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGZ2702317.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            2563..3306
                     /gene="yaaA"
                     /locus_tag="ACOIAS_002616"
     CDS             2563..3306
                     /gene="yaaA"
                     /locus_tag="ACOIAS_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGZ2702318.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            3404..6091
                     /locus_tag="ACOIAS_002617"
     CDS             3404..6091
                     /locus_tag="ACOIAS_002617"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HGZ2702319.1"
                     /translation="MKKRKEWSSKMENHKQMPWHSRATHEILESLDSSEEGLTDTEAE
                     KRLKENGLNELRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIV
                     TVNAVIGIVQEKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAM
                     VPADLRLIETSSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGR
                     GVGVVVATGMDTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGA
                     FYQRPLLPMFMIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGG
                     ATVICCDKTGTLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCN
                     DACINPDKTDEILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVH
                     NIDEKIIAYTKGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGF
                     AKREISSIPKEDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHK
                     VTATTIAHELGIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSL
                     KRIGEVPAMTGDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIK
                     EGRRVYRNIQKVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALA
                     LGVDPPSRNIMKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQ
                     TMAFCVLAFSQMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFAPVFRN
                     AFNLTVLNNLEWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      6145..6247
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6357..7622
                     /locus_tag="ACOIAS_002618"
     CDS             6357..7622
                     /locus_tag="ACOIAS_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HGZ2702320.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            7652..8542
                     /locus_tag="ACOIAS_002619"
     CDS             7652..8542
                     /locus_tag="ACOIAS_002619"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGZ2702321.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            9030..9473
                     /locus_tag="ACOIAS_002620"
     CDS             9030..9473
                     /locus_tag="ACOIAS_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2702322.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            9504..11321
                     /gene="ftsH"
                     /locus_tag="ACOIAS_002621"
     CDS             9504..11321
                     /gene="ftsH"
                     /locus_tag="ACOIAS_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGZ2702323.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            11499..14201
                     /locus_tag="ACOIAS_002622"
     CDS             11499..14201
                     /locus_tag="ACOIAS_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2702324.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            14235..14933
                     /locus_tag="ACOIAS_002623"
     CDS             14235..14933
                     /locus_tag="ACOIAS_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702325.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            15290..16246
                     /locus_tag="ACOIAS_002624"
     CDS             15290..16246
                     /locus_tag="ACOIAS_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HGZ2702326.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT         6395 a         1818 c         2930 g         5106 t
ORIGIN      
        1 atttgtccta gttgtaatga aactcacgat agagatataa atgcaagtat aaatatatta
       61 aaagaaggat taagactaat aacaattcaa aataaataac gtatgtaaga accgtaggaa
      121 ctacggggat agcctgtgga gaattagtaa gacatatttt agtatgcaaa attctgtgaa
      181 gcaggaaccc tgtgacttta gtcatgggag gttcagtgta gcaataaata tatatcacct
      241 ggctttattg atgcacatat gcatattgaa tctacaatgg taatgccaat tgaattatcg
      301 aaaaaattat taaaatcagg tacgacaact atcatagccg acccacatga gttggtcaat
      361 gtaaaaggag ttagtgcaat agatttttta ttagaatcga caaaagatat accacttaat
      421 gtatatatta tggtgccttc atccgtacct gcaacaagtt ttgaaactaa tggagttggt
      481 aaatttagtg caaaagacat ggagagttat gtaaataatc ctagaatttt aggtttagga
      541 gaggtaatgt gttttaatga tgttataaat tctgaaaatg aaatcttgga taaactagag
      601 ctttttaaaa ataaagttgt agatggacat gcacctaata taaatggtaa atcacttcag
      661 acatatgtat gtgcaggaat agaaaatgac catgaatgta taacttttga tgaagtatat
      721 gaaaaattga gagcaggatt aaaaatttta ataagagaag gaagcgcagc aaaaaattta
      781 aagtctatag taagtggtat gctaaagcat aacttgccaa ttgaagaatt tatgttttgc
      841 acagatgata agcacttaga tgatatagaa aaacaaggtc acataagatg gaatataaaa
      901 tgtgcgattg atttaggtat ggaacctgtt agggctataa aagttgcaac ttataattct
      961 gcaaaagcat atgggttaag gaaaattgga gcaataggtg cgggttataa ggcagatatt
     1021 gttgtattga atgatttaga caaaatggaa gtggatagtg tatataaaga tggtaatttg
     1081 gtaaacgaag agatgttttc taattacaat tatgagatta aagataaaga attgcttaat
     1141 acagtcaagt ttaaatatat taataaagaa aaaatacagt taaaagtaag tgaaaaaaat
     1201 tatgtaatgg agattgtacc ttatcaaatt ttgacaaata aggtatatga atctttacca
     1261 tgtgcagatg gatattttgt tccaaataaa gagtattcaa aactttgtgt tgttgagaga
     1321 catagaatga ctggaaatgt agcagttgct ccactaaaag gatttggcat aaaaaatgga
     1381 gcaattgcta ctactgtggc ccatgattct cataatatta tagttgcagg ggataatgat
     1441 gatgatattc tagtagctat aaattactta aaggaaatac aaggtggtta tgtaattgta
     1501 tcaaatggaa aagttcttgc acacctaagt cttcaagtag ctggattaat tagtactttt
     1561 acagctgaag aagtacaaga aattactgat aatatgttgg agatagcaag aaaaatggga
     1621 gttccagagt atgtagaccc ttttattaca ctttctttta tggcacttcc agttgttcca
     1681 caaataagac ttacagattt aggtctattt gatgttgagg aatttaaatt tatatagata
     1741 atataaaagt aataataaat gaggctttct caaaataggg ataagtctct ttttattgtt
     1801 gaaatgataa aaatatattt gatttcatac aaaaaagagc atctcatgaa ctaaaaattt
     1861 tatttttaga tatccttatt ttaattaatc tatcatttaa ccttgtttta agcatagata
     1921 tcttaataaa actattttca gtctatttta acatataaat acatgaaaat tatatattta
     1981 tgaaacttta tacataaaaa ataaaatatt ttatttatat atagatttat agggtaataa
     2041 tataagcata gtataaaatt attaataaaa acaaaaggag agatatttat gttgagtata
     2101 ggaacaaaag caccagaatt tacactagaa gataaagatg gaaataaggt aagtatgtca
     2161 gattttaaag gcaaaaaagt agtagtatat ttttatccta aagataatac accaggatgt
     2221 acacgtcaag cttgtgcttt tagaaatgca tatgatggat ttaaaaagga agatatacaa
     2281 gttattggga taagtaaaga tagtataaaa tcacatcaaa aatttgcaga aaagcatgaa
     2341 cttcctttta ttttactttc tgaccctgac ttagttgcta taaaagcttt tgatgtatgg
     2401 aaagagaaaa aaatgtatgg aaaaactgca ttgggagttg tacgtgcgac atatattata
     2461 gatgaaaatg gaattattga aaaagtcttt gaaaaagcaa aaccagacac aaatgcacaa
     2521 gagattcttg aatatttgga aaaacaggag taataagaat ttatgattat aatgttatca
     2581 ccagctaaaa atatgaagaa tatagaagta tttgatagag atttgagttt gccttgcttt
     2641 atagataaca caaaagaaat agtggaaaat ataaagacat ttggtataga agattttaaa
     2701 aataaaatga aaattaatga aaaattagct gtattaaata aaaatagatt tgaaagcatt
     2761 aaatttgata gattaggaaa ccctgcaatc ttaacttatg atggaataca atataaaaat
     2821 atagaagcag aaaattttac aaggaaagat gaagagtttg caaatagctg tattcgaatc
     2881 ataagtggat tgtatggtgt tgtaaaacca tatgatagta tatatgaata tagattagaa
     2941 atgcagacaa aattacgagt aggtgagttt aagaatttat acgaatattg gggaaatcgt
     3001 atatataaag aactaatcaa agaaaaaaca gctatcgtta atctttcatc aaatgaatac
     3061 agcaaatcca tagaaaaatt tattaaagat agtgatacat atattacatg tacttttaaa
     3121 gtaaataaaa atggcatatt aaaagtggaa tctacacaag caaagaaagc tagaggtatg
     3181 atgacaaaat acattgtaaa aaataggata agagatattg aagaactgaa aaagtttaat
     3241 ttagaaggtt ataagtataa agaaaactta tctaacaata gtgaatacat atttgtaaaa
     3301 gagtagaagt tttgttaaat attaaatggc attgttaaat ttaggtaaaa tatacttagt
     3361 gtgtattgaa ttgaacttga ttttaaaata taattattct taaattaaaa aaagaaagga
     3421 gtggtcttcc aaaatggaaa atcacaaaca gatgccttgg cattcaagag caacgcatga
     3481 aattttagaa agtctagatt catcagagga ggggctcacc gataccgagg cggaaaaacg
     3541 gcttaaagag aatggcttaa atgaacttag aaaaaaacca cctagaacaa ctttacaaat
     3601 gctttggagt caaattacag atgctatggt tatgatacta ataggagcag caatattatc
     3661 tttgatattt ggcgaattta cagaagcttt tgtaatacta ataattgtaa cagtaaatgc
     3721 agttattgga attgttcaag agaaaaaagc agaatcatca ttagaagcat taaaaaatat
     3781 gaactcacct acagctcgtg ttatgcgtga tggtgaagaa aatgttattc cagcaagtaa
     3841 tcttgtagta ggagatattg tttttttaga agatggagct atggttccag cagaccttcg
     3901 tttaatagaa acatcaagtt taaaaattca agaggcttct ttaacaggag aatctgttcc
     3961 atcagaaaaa gatgcagata caatcttacc taaagaatgt gtattaggag accgtagcaa
     4021 tatggcgtat acatcttcaa tagtcacata tggtagaggt gtaggtgttg tagtagcaac
     4081 tggtatggat acagaagtgg gtaacatagc acatctttta gattcacaag atgattttga
     4141 tactcctata aaacgtaaac ttaatactgt tggaaagaca ttaagtgtca taggtattat
     4201 tgtatgcgtt gttatatttg caataggtgc attttatcag agacctctct tgccaatgtt
     4261 tatgattgca atttcccttg ccatatcaat tattccagag ggattacctg caacagctac
     4321 tattgtaatg gctttaggcg tacagcgtat ggctaaaaga aatgctttaa ttagaaaatt
     4381 acctgctgtt gaaacattag gaggagctac agtaatatgt tgtgataaaa ctggtacgtt
     4441 aaccttaaat aaaatgactg ttacacatat tgctgtcaat ggagattttg aatctggaaa
     4501 agtgacatca atagatactg catctgaaaa acatccatta gtgtataaag agattgttta
     4561 tgctggtgca ctttgtaatg atgcatgtat taatccagat aaaacagatg aaatattagg
     4621 tgacccaaca gagggcgcat taatttttat ggccaaaaaa tttggaatag atcaagaagc
     4681 tcttgaagaa gaatatccac gtgtatttga acagcctttt gattcagata gaaaatgtat
     4741 gacaactgtt cataatatag atgaaaagat tattgcttat actaaaggag cagtagacga
     4801 aattcttgac ctatgtacta aaatattaac ttcaaaaggt gaaagaaaca ttaccgaagt
     4861 ggataaaaag aatatacacg aattatgtct taacatgtct aaagatgcat tgcgtgtact
     4921 tggttttgct aagcgtgaga tttcatctat accaaaagaa gatagtgaaa atatagaata
     4981 tgatttaaca tttataggta tggttggtat gattgaccca ccaagaactg aggtaattga
     5041 tgcagtaagt acttgtaaag aagcaggtat aagaaccata atgataacag gtgaccataa
     5101 agttactgcc acaacaatcg ctcatgagtt aggaatatgg agtgaagaaa atactgtgat
     5161 atctggtgat gaacttgata atttgagtga tgatgaacta gatgaagctg ttaaaaatac
     5221 tacagtattt gctcgtgtat ccccatttga taaacttaga attatacaat ctctaaaaag
     5281 aattggagaa gtaccagcaa tgactgggga tggtgtaaat gactctcctg ccttaaagtc
     5341 tgctgatatt ggtatagcca tgggtataag tggtacagat gttgcaaaag attcttctga
     5401 catgatttta atggacgata gttttacaac aattgcttat gcaataaaag aaggtagacg
     5461 tgtatataga aacatacaaa aagttataca atttttgctt gttggtaata tagcagagat
     5521 acttacgttg tttgtagcaa tattattaaa ttgggatact ccgcttcttg ctgttcatat
     5581 tctatgggta aaccttgcaa ctgctacgct tccagccctt gctcttggag ttgacccacc
     5641 aagcagaaat ataatgaagc accagccagt gaaatcaacc actttgtttg aaaaaaatct
     5701 tatacaaaga gttgttaccc aaggtatatt tgtggcaata atgactattt tagcatataa
     5761 aattggtctt aatatagaaa atcattctgt aggtcaaaca atggcatttt gtgtattagc
     5821 attttcacag atgttacgag catttagtca gcgttcaaat acagattcaa tgtttaatag
     5881 aaataatggt aaaaatccat ttcttttaat ctcattttta acttctgcat ttttaatagc
     5941 agtaatacta tttgcacctg tttttagaaa tgcttttaac ttaacagttt taaataactt
     6001 agagtggctt ataacgattg ttttagccat aatgtctgta attcaagtag aggttgggaa
     6061 aattattaaa agaaaaaaat taaaaaaata gttgacaaaa ttaggaaaaa tatataagat
     6121 ataaaacata gaaaaattaa ataaatctta tccagagagg tggagggact ggccctatga
     6181 aacccggcaa ccagtatatt tatatacagt ggtgctaaat cctgcggcga aagctgagag
     6241 atgagtataa ttaagcttat acagagcctg agttatatac tcaggcttta tttattttaa
     6301 ctctcatctg gtaaatagtt taattttttt tactaaaatc taagggggat acaagaatgt
     6361 ataataaaga aacaatatgt gtgcaaggaa attataaacc aggtaatgga gaaccaagag
     6421 tactaccttt atatcaaagt acaactttta aatatagcag tatagaccaa cttgctgaat
     6481 tatttgattt aaaagttgat ggacatatat attcaagaat aagcaatcct actattcaag
     6541 cttttgaaga aaaaataagt ttactagagg gtggagtatc ttctgtagct gtatcatcag
     6601 ggcaatctgc aaatatgttg gcagttttaa atatatgtaa atcaggagat agtatacttt
     6661 gttcttcaaa agtatatgga ggaacattca atttactagg acctagtctt aaaaaatttg
     6721 gtatagattt aatatcgttt gacttagatt caagtgaaga tgagatagta gaacttgcaa
     6781 aggaaaatac taaggttgtg tttgcagaaa cacttgcaaa tccaactctt gaagtcatag
     6841 attttgaaaa aatagcaaat gtagctaaga gaattaatgt tccatttatt gttgataatt
     6901 cattagcatc tccagtgctt tgtaaccctt taaagtatgg agcaaatata gttactcatt
     6961 ctaccacaaa atatttagat gggcatgctt caagtgttgg aggaattata gtggatggtg
     7021 gaaactttaa ctgggataat ggaaaatttc cagaattagt tgagccagac ccaacatatc
     7081 atggtataag ctatactcaa aaatttggaa atgccgcata tgcaactaaa gcaagagttc
     7141 agttgcttag agactatgga aattgtttaa gcccattcaa tgcgtatctt actaatttaa
     7201 atgttgaaac actacatctt agaatggaga gacatagtga aaatgcactt aaaatagcta
     7261 gatttttaga aaaacatgaa aatgtagatt ggattaatta cccaggactt gaagataaca
     7321 agtattatga gaatgccaaa aagtatttat caagaggatg tagtggtgtt ttatcatttg
     7381 gagtaagagg tgggttagaa aatgccaaaa aatttgtgga aaaattacag atagcatctt
     7441 tggttacaca tgtttcagat gtaagaactt gtgttataca tccagcttca actactcata
     7501 gacaattaac agaggaacaa ttaattgcat ctggagtatt gccttcacta ataagattat
     7561 ctgttggaat agaaaatgta gaggatttaa tagctgattt aaatcaagct ttaaatttct
     7621 aatattctaa gagaaagaga agtgaaaaga tatggcttta atactaccaa agggattacc
     7681 tgttattaat aaattattag atgaaggaat agatgtaatt tataaagaag attttaaaaa
     7741 ggaattaaag tatgaagaaa acatagacac taagatagct atactaaatc taatgcctat
     7801 aaagatagat acagaattag atttactaag aaggatagat aaaacaggat tcaacgtgtc
     7861 tgtggaattt attaaaattt caacaagaga aagtaaaaga agttgtaatg aatatgttaa
     7921 agaattttat aaaacatttg atgaagccaa aggagagtat tttgatggct ttataataac
     7981 aggagctcca gttgaacaaa tggagtttga agaagttgat tattggaatg aactagaaga
     8041 aattatggat tattctaaga gaaaaactaa atctacatta tatatatgtt gggcagctca
     8101 agcaagtctt tacaaatact acaatgtaaa aaaacttcca ctaagtcaaa aatgttttgg
     8161 agtatttaag cataaggtag ataaagatag taaaatagta gatggatttg aaaatgaatt
     8221 ttttgctcca cattcaagac atactactgt aaatattgaa gctttgaaag agaataaaga
     8281 gttgagcatt gtgagtcatt ctaaagaagc tggtccctat ataattacaa attcaagaga
     8341 tgtgttcgta atgggtcata gcgagtatga caaatacact cttgataaag aatataagag
     8401 ggatataaat aaaggagata aaatatctat acctcaaaat tactatatta atgatgaccc
     8461 ttcagaagag ccaactgtaa aatggaaaaa acattcagaa ttgttgttta gaaattggat
     8521 aaaaaactat ttgatacaat aaattttaag agtatataaa tgttaactaa aaaaatatag
     8581 aaatttatag gtataaagtt aatcttaatg atactttata ccttattttt ttaaaatttg
     8641 accttataat agtagtttta tattttaaga aaatcacttt aggtgtatgt agatagaggt
     8701 ggctttaatt ttatatatgg tgcgataatg ggagactaaa cctttatagt atcaataaat
     8761 ttgtatttta acttttaaaa gagattaaaa agggagaaat cagtgtaaaa tatatataga
     8821 ttttttatta aattttatta aaaatatcct gtataaaatt aaatatcaat aagataacaa
     8881 aatttcaatt gaagttacaa tattgtaatt gacaataaat acttaatact ataatattta
     8941 atacattaaa cattatagtg ttaagtattt attacagtat gcacttcagc ctaaatttga
     9001 aataaagtat tttcgtaggg agcgtttgta tggatgagat gagttttaag aaagagctac
     9061 tagagttgac tagagatata aatatgaaat ttacaacttt attaagtgat ttttatcagc
     9121 cacttggtat tacagcagtg caagctttga ttttatctga gttatgtgaa catggagaga
     9181 aaaaaataag cgatttaggg aagaacttaa acatgacaaa tagtaatgta tcagtaatct
     9241 gtcaaaggct tgaaaaaaat ggctttttaa atcgcataag agatatagaa gaccaacgaa
     9301 ttgtaaaagt aaaagttaca aataaatcat tggatataca agaacatatt tcaagtagta
     9361 tttttgatag ttattttgaa aatatgacca gtgaaaaatt gcaagatatg gaagatataa
     9421 tagaaggttt agagaaatta aataaacttt taacttacat tgattgcaaa taacataaaa
     9481 ataatttaaa ggagaataaa attatggatg aacataaacc ccaaaaaagg tcagcactct
     9541 attattatac agttataata atagtagtaa tgtttttaaa tgctttaata tttccaagtt
     9601 taatggataa aaaaatggtt gagattgact atggaaagtt tctcacaatg ttggatacag
     9661 gaaaggttaa atctgttgag atagaaaata atagaatagc aataaatcca tctagtagca
     9721 aggataagaa tatatacata actggtagaa tggatgaccc agaattggta gatagactaa
     9781 aaaatgcaaa agtagaattt actaaggtta ttcctaagga aaattcacct cttttaagta
     9841 ttttacttac atggataata cctataggta ttcttatgct atttggtaac ttaatgatga
     9901 agtctatgca aaagcgaatg ggtggtaatg ctatgcaatt tggaaaaagc aacgccaaaa
     9961 tgtatgtatc tgctcaaagt ggaaaaagat ttaatgatgt agctggtcaa gatgaggcaa
    10021 aagaagcttt aactgaaata gtagatttct tacacaatcc agaaaagtat aaaaagatag
    10081 gagcacaaat gcctaaagga gcacttttag taggacctcc aggtacagga aaaacactcc
    10141 ttgcaaaagc tgtagcaggt gaagcaaatg taccattttt ctcaatatct ggttctgagt
    10201 ttgttgagat gtttgtagga atgggagctt ctaggattcg tgatttattt aaacaagcaa
    10261 aagaaaaagc tccatgtata gtgtttatag atgagattga tactattggt aaaaagcgtg
    10321 ataatggaag tggattaggt ggaaatgatg aacgtgaaca aactttaaat cagttgttaa
    10381 gtgagatgga tggttttgat ggtggaattg gagtagtaat acttgctgct acaaatagac
    10441 ctgacagtct tgacaaagca ttacttcgtc caggaagatt tgatagacgt ataccagttg
    10501 aacttccaga ccttggagga cgtgaagcta tattaaaggt acatgctcaa aatgtaaatg
    10561 taagctcaga tatagattat aatgcaattg caaggtctac atcaggagca tcaggtgcag
    10621 aacttgcaaa tattgtaaac gaagcagcac ttttagcagt taagtctggc agagatatag
    10681 tagaacaaca ggattttgat gaatctgttg aaactgtaat tgctgggtat caacgtaaag
    10741 gagctgtaat ttcagataag gaaaagaaaa ttatagcata tcatgaaata ggtcatgcac
    10801 ttgttgctgc tatgcaaaaa cattctgcac ctgtccataa gattactata atacctagaa
    10861 cttcaggagc attgggatat actatgcaag ttgctgaaga tgagagtgta cttatgtcta
    10921 aagaagaagc aattgataaa ataactactt ttacaggtgg tcgtgctgct gaagaggtta
    10981 tctttaacac ttgtacatca ggagcatcaa atgatataga acaagctact aaaattgcaa
    11041 gagctatggt tactcgcctt ggtatgagtg aagagtttga tatggtcgct cttgaaactg
    11101 taagcaatca atatttagga ggagatactt ctcttgcttg ttcttccgac actgctgcta
    11161 aaatagatga agaggtattt aaaatagtaa aggcttgtca caaaaatgca attgaaatct
    11221 tagaaaataa tattgataaa ttgcatgaac ttgctaatta tttattagaa aaagaaacta
    11281 ttacaggtga tgagtttatg gaaatactta acttaaacta aaatgattat acacaagata
    11341 aaaatgccta ttgttaggca tttttatttt acaactaaat tttttaaatt catatttaaa
    11401 tctttacata atcttaatca ttaaaaatac atcttaacta caattttata tcggaattga
    11461 tattataata gtaatgaatg gtgtaaggag gttttttaat ggatagacct aatcctgata
    11521 ttctattaga aaaaattaaa aatgaagaag aaaaactaag tagaggtcaa ttaaaaatat
    11581 tctttggtta tgcagctgga gtaggaaaaa cctactctat gttggaatct gcacaaaatt
    11641 taaaaaaagt gggtgtagat gtagtagttg gatatattga accacacact agaccagaaa
    11701 cattggcttt acttgatggc ttggaaattc ttcctgtaaa ggagatagaa tataaatcta
    11761 taaaactaaa agaatttgat ttagatgctg ctttagcaag aaaaccagaa gttatattag
    11821 tagatgaatt tgcccattct aatgttgctg ggttaaggca tacaaaaaga tggcaagata
    11881 tagaagaatt gctgttagca ggcataaatg tctacactac tgtaaatgta caacatttag
    11941 aaagtctaaa tgacattgtt gagataatta caaacgtatc agttaaagag actataccag
    12001 ataagttttt ggatacaaat acacagttag aacttataga tgttgaacct gatgtacttc
    12061 ttgaaaggtt caatgaaggt aagatatata agaaagaaca agctactaga gcaaaaaata
    12121 atttttttat aaaagataac ctggttgcac ttagagaaat cgcacttaga aaaactgcag
    12181 agagagttaa taaagaagtt caaatgacca gactatcaaa gggtgatgta actgtaatac
    12241 ctacatcaga tacacttctt gcatgtataa gtccatctcc atcttcagca aaagttatta
    12301 gaactgcatc cagaatatca gacagtacat ttgcaaaatg gatagcttta tatgttgaaa
    12361 ctccaaacac agcaagactt ccatttgatg aacaaaaaca actacaaaat aacctaaaat
    12421 tagctaagaa attaggtgga gaaataattg ttttacatgg tgaaaatata atagagcaaa
    12481 tacttagaat tgcaaagctg agaaatgtaa caaaaattgt aataggtaga aatcattcaa
    12541 gtaataaaag atttagtaaa agatttaaaa aagatatagt tgacaaatta attgatgaag
    12601 ttgattatat agatatacat ataattccat ataaatttgc ctcagatgtt aaatacaaac
    12661 ctaaaaagga taaatcatca ataaaatcta aatttaaaat tagcaaagtt gattttataa
    12721 aacttatatt tattactttg atgataagta ttttagcata tgcagtacaa agcatgggat
    12781 ttataagaga aaatatattg ttaatatata tgttgggtgt agttttagtt tctatgtgga
    12841 ctaaagggta tagtacaggt ataataagtt ctgtatttaa tatagttctt ttaaattatt
    12901 ttttcacagc accattatat acattatcta tagcagattc aaactatata gtaacactgg
    12961 ttgtattctc aatagtaggt ataataacta gcactcttac gtctaagata cagcatgaag
    13021 cagaaactgc tgctaaaaga gaagaaaata caaaaatgat atatcaaata attaagggat
    13081 ttttaagatt atcaaataaa gatgatatag taaacaaagg tatagaactt ttgtctctta
    13141 gcttaagtag agatgtaatt tgttatttaa atgtagataa gcataataaa tcaaagcttt
    13201 acaaaaaaaa tactaataat aaggataaaa atgatttgga tagtgaagat gaaaaagctg
    13261 ttgcaagttg ggttttatcc aattcaacag tagcaggaaa tgacacggat acacttcctg
    13321 gttcaaaggg atactatata ccaattgtag gaatgaattt aactttagga gtagtgggaa
    13381 tatcatgtat tgattcaaaa ttggatacag aagatataag cttaattgaa acaataattg
    13441 ctcaaatggc aatagcgctt gatagagaaa tcttgtcaga agctaaagaa aataccaatc
    13501 ttgagattga aagggaaaga ttaagaagta atcttttaag agcagtttcg catgacctta
    13561 gaactccact agcaggaata tctggtgcag taagtacaat tataaaaaac aagggtacta
    13621 taggtcaaga tataatagat gaattactta gtggtgtata tgaagatact cagtggttaa
    13681 taagacttgt tgaaaattta cttagtatga ccaagataga tgaagggaaa ttggaagtta
    13741 aaaaacattc tgaactggtt gaggaaatta tatcagaagc gttacaaaaa ataaaaaaaa
    13801 gaattgaaaa tgcacttata gatattaata taccagaaca gatattattt gttcctatgg
    13861 atgcaaaatt aatagaacaa gttttaatta atttaataga taattctttg aaatattcaa
    13921 aggaagattg taagatagag ataaatgtat atgagaaaga tgattatgtt tggtttgaag
    13981 tatctgataa tggtcctggt atatcaaagg aacttaaaaa gcatatcttt gacagatttt
    14041 ttactggaga agaaggagct aaagattcca gaaagggtgt tggacttgga ttatcaattt
    14101 gtaaatctat aattcaagct cataaaggtg aaattatggt agaaaacaat aaagataagg
    14161 gttcaacttt taagtttagt ttgcctaagg aaaatgaata gttttaaatt ttaaaattag
    14221 ttttggagga agaaatgagt aaaaaactta tattgttagt agaggatgat aagacaataa
    14281 gaaaatttat atctactgca cttttaacac aggattatga tgtcaaggag gctataactg
    14341 gaaaagaagg tatatctata gctgtctcat attctccaga tgttgttctt ttagatttag
    14401 gcttagaaga tatggatgga atagaggtaa taaaagctat cagacaattt tcaaatatac
    14461 caataattgt agtttcagct agagagcaag atagagacaa ggtagaagta tttgatgcag
    14521 gggcagatga ttacttaaca aaaccattct ccatagtaga gttattagca agagtaagag
    14581 tagcatttag acattcacaa gtagaagctc aacaaaaaga tgatgttaaa tctacgtttg
    14641 aagtagataa attattgatt gattttgata aaagaaaagt tattgttgat gatgttgaag
    14701 tccatttgac acctattgaa tataatattc tttcattact tgcaaagtat catggaaaag
    14761 tgcttactca taattttata ataaaagaaa tatggggtag tgttataggg aatgagacta
    14821 agtctttaag ggtctttatg gcaacgttaa gacgaaaaat agaaaaacaa cctgcaaacc
    14881 caagatatat aattacagaa gttggtgtag gatacagatt aaatgatgaa tagttgtaat
    14941 atattaatta ttagattatg tgcttattaa tatatattaa aattatatag tttagttaaa
    15001 ataagaatga agtgtatgtg cttgttgtca gtttagcaca tgcattttat tttttagctt
    15061 ttttagtatc aataattttt tagtatcaat aatatgaaat atgttttaaa ataagtatat
    15121 agtgtaatct gtaataaaaa ctttggttaa ataataaatg tatttaacac ttaccttata
    15181 ataaatttaa tataaatatt aaataataat tattaaaata gtgtaaaata attacagtaa
    15241 ggattataaa aaatttttag aatgtaaatt gaaatttagg agtagatgta tgtatatact
    15301 gaatttaaat acaagagaga ctattgaaga ttttagagat aaattttatg tagcagaaaa
    15361 ttcttatctt atattaagtg ctccaaaaaa tttaaaattg cttaaggaaa ctttggatat
    15421 agatgaaata acttttaatg attgtcttaa atttgatgaa ataaccaaac ttgatttatt
    15481 tgataattac gattttttaa gcctaaatac atttgaacta agagatggag aagctgttat
    15541 agaagaggta aatatgtatt tatcagataa ttttatattg gttgtagtga atgaagaaca
    15601 ctttttattt gaatttgtaa aaaatattat tttgaagaac tctcaacttg aaaaaaatcc
    15661 agttattaat ctatttaaga taaactactt gattcttaga gaagttataa aaaatggttt
    15721 tgagtctttg gaaaaagttg aagagttaat tcttcaaata gaagatgaaa tgatggacaa
    15781 cattaataag aatcatgtat ctagaataag tgatgtaaga ggattaacaa gaattatagt
    15841 taagaatact agacctcttt tgtatattgg ggatagaata gttaaggaaa atataagata
    15901 tttaaaatat tcaaatgtaa aaaaatataa tttagaaaat tttcaaggta tagattttgg
    15961 aattgacaaa ttatacaatt tcgcattgtc aacacgagag ttagcagata aattattaga
    16021 tatttattct tccagagtag gtgagaaaac aaataactta attacaaaat taaccttgtt
    16081 aacagccata tcagcaccac ttacaattat aactggtata tatggaatga actttagata
    16141 tatgccagaa ttaaactgga tttatggata tccagcaact ttatttttta tgctatgtat
    16201 aatttttgtt ggaattataa tatttaaaat aaaaaaatta ttgtaaatt
//
LOCUS       DBGJFU010000089        15543 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.089, whole genome shotgun sequence.
ACCESSION   DBGJFU010000089 DBGJFU010000000
VERSION     DBGJFU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.089"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            419..985
                     /locus_tag="ACOIAS_002625"
     CDS             419..985
                     /locus_tag="ACOIAS_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HGZ2702327.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            1290..1751
                     /locus_tag="ACOIAS_002626"
     CDS             1290..1751
                     /locus_tag="ACOIAS_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2702328.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1819..4065
                     /locus_tag="ACOIAS_002627"
     CDS             1819..4065
                     /locus_tag="ACOIAS_002627"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702329.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            4058..5890
                     /locus_tag="ACOIAS_002628"
     CDS             4058..5890
                     /locus_tag="ACOIAS_002628"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702330.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVE
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6615..7886
                     /gene="hflX"
                     /locus_tag="ACOIAS_002629"
     CDS             6615..7886
                     /gene="hflX"
                     /locus_tag="ACOIAS_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGZ2702331.1"
                     /translation="MAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKLEL
                     DRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVDVLDR
                     ILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNENMS
                     IINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            8119..8616
                     /locus_tag="ACOIAS_002630"
     CDS             8119..8616
                     /locus_tag="ACOIAS_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2702332.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8666..9190
                     /locus_tag="ACOIAS_002631"
     CDS             8666..9190
                     /locus_tag="ACOIAS_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HGZ2702333.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            9502..10866
                     /locus_tag="ACOIAS_002632"
     CDS             9502..10866
                     /locus_tag="ACOIAS_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGZ2702334.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLVAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            11005..12171
                     /locus_tag="ACOIAS_002633"
     CDS             11005..12171
                     /locus_tag="ACOIAS_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGZ2702335.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            12288..13070
                     /locus_tag="ACOIAS_002634"
     CDS             12288..13070
                     /locus_tag="ACOIAS_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2702336.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(13189..13842)
                     /locus_tag="ACOIAS_002635"
     CDS             complement(13189..13842)
                     /locus_tag="ACOIAS_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2702337.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(13866..15230)
                     /locus_tag="ACOIAS_002636"
     CDS             complement(13866..15230)
                     /locus_tag="ACOIAS_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HGZ2702338.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         6071 a         1681 c         2595 g         5196 t
ORIGIN      
        1 tagatatgta gaatatattt tgaaaattaa aaaaattttc tattttatat attgaacaat
       61 ttatgtaaat agaaaatttt tcaataagac ttttgagttt aatcattata ttggtaaggt
      121 aaacattttt aaatctaatt atatataggt gaaataaaaa attaattata agtaaggcat
      181 ttagaataaa attttaaaaa aattataatt aaaaaaataa tgaaaatata taatattagt
      241 ttagattata aaaaataaat atacaaatac ttataaatac aagttatata caagatttat
      301 agtagagtaa taatcttata aaataattta ataaatataa attaaattat atgtgtatat
      361 attgtacttt aatagtgata taataataaa aaaattttta aagagggtga tattacatat
      421 gagaaaactt gaagagatat tagaatataa taaatcattt gtagagaaca aagaatatga
      481 acaatatgtg acatcaaaac atccaaataa aaaaatagta gtcttatctt gtatggatac
      541 aagattaact gagcttttgc ctaaggctat gaatttgaaa aatggagatg tgaaactaat
      601 aaaaaatgca ggagctacta taatgcatcc ttttggaagt attatgagaa gtgttttagt
      661 tgcaatttac gaatttgatg tagatgaggt tatggttgta gggcatcatg gatgtggtat
      721 gtgtaatgtt gatacggata agttacttgg taaaatttta gatagaggaa tatctaatga
      781 tgtaatactt acacttcgta gtgctggaat tgatacaaaa caatggttac atggattcga
      841 ttctgcagaa gaatctgtaa aagatagtat tgatttaata aaagggcatc ctttaattcc
      901 agatggtatt attgtacatg gtctaattat atcacctgaa actgggaagt tagatgttgt
      961 tgtaaatgga tatgaagata agtagagtat cataatgtta tataatcaag atactgttga
     1021 ataagatgtt gtttcaaaat ttattttggg acaacttttt tatttgaaaa tatatgaaaa
     1081 ggtttatttt aagctttaca ataaaaaata taagtagctg actaattaaa aatcttacta
     1141 ttatttatta aaattataac tatatgatat aaaaatattt aataaatgaa atttatatga
     1201 attttttata aatatattga aaaaatgcat gcagtatagt aaaataatgc ttagccgact
     1261 aagtaatata aaaaataagg agggcaagta tgaatagcaa ttctgaagaa aaattaaatg
     1321 atgttttctt gcaattaatg cgaatatatt ttataagaac tcatttttat tttgataaat
     1381 taggcttata tcatggtcaa ccccaaatac ttttttcttt aaagatgaaa gatgggcaaa
     1441 gtcaaaaaga attagcagat aaaaggaaag taaaagcatc tacaactaca gttatgataa
     1501 agaggatgga aaaagctgga cttgtggaaa gaaaacaaga tgaaaaggac caaagagtat
     1561 ctaggatttt tttgacagac aaaggtttag aaatatgtaa taaagttgaa gaatttaaca
     1621 aggaattgga aagagagttt tttttaggat ttagtgaaga agaaaaaata gtacttagaa
     1681 gacttctaat gcaagtacga gataatttaa aagcaggatg tcataagcat gaagatgaaa
     1741 aatattttta aagtttaaaa taatgttttt caatttaatg ttattagact aattaatatt
     1801 acagaaggga agaaatgtat gtttaaatta attaaatatt taaagaaatc ggcattatca
     1861 atagtaataa tagtctgtct attggtaatt caagcggtat gtgacctttc tttaccagaa
     1921 tacacatcca atatagtaaa tgtaggtata caacaaggtg gagtagaaaa ctctgttcca
     1981 tctgtgatta gagagagtga attaaataaa attactttat ttatggataa aagtagtaag
     2041 gataaagtac tagataatta tactttgctg aataaaaagg attatgttaa gtataaagat
     2101 aagtacccag gtctaaaaga tgaatctctt tatgagttaa atactaaaga caaagacact
     2161 atagatgatt taaatgttat ttttggtaaa gctattttaa tagtatcagg gcttgagggc
     2221 gatagtaaag aggtaaaagc tatgaaagct cagttaatga gtaaacttcc accacaagct
     2281 actcaaagtg gagatgttga tatatttaaa ttgttatcag caatgccaaa ggaacagtta
     2341 gatacattga ctaaagagat gagcaaaggc tttgaaagta tgccagagag tatgataaca
     2401 caaagctctg tttcatatgt aaggtcagaa tatgagaaaa taggtattga tacagaaaaa
     2461 actcaaaata attatatatt atttactggt gctaaaatgc ttggtatagc aatgattagt
     2521 atggtagcta caataacagt aggattttta gcagcaagag tagccgcaag tgtaggtaga
     2581 aatttaagaa gtggagtatt tagaaaagtt atgtcatttt caaatactga aatgaatgaa
     2641 ttttctacag cttcattaat tactcgtagt acaaatgata ttcaacagat acaaatgttg
     2701 atggttatgt tgcttagaat agtattctat gcacctataa tggctatagg tggagtgata
     2761 aaggttttaa acacaaatac ttcaatggcc tggattatag cagtagcagt agttgctatc
     2821 ttatcattaa ttgtagtgtt attctcagtg gttatgccta agtttaaact ggttcaaaag
     2881 cttgttgata agttaaactt agtaagccgt gaaataatta ctggtattcc agttatcaga
     2941 gcttttagta ctgaaaaaca tgaagaagaa agatttgata aagcaaataa aaatttaact
     3001 aaaacaaatc tttttgtaaa tagagttatg acatgtatga tgcctgctat gatgcttata
     3061 atgaacctta tcacagttct tatagtttgg agaggtgcat acagtgttga ttctggagca
     3121 atgcaagttg gagatatgat ggcatttata caatatacaa tgcaaataat aatgtcattc
     3181 ttaatgatat caatggtatc tattatgctt ccaagagcat ccgtttcagc tacacgtata
     3241 gatgaagtat tagtgtcaga tataagcata aaagacccag aaaaatcaga ggcacaagag
     3301 tttaaggaag ataaaaaggg tcttgttgaa tttaaaaatg tttcgttcat gtatccagac
     3361 gcagaagaac ctatattaac aaatatatct tttacagcaa agccaggaga aacaactgcc
     3421 tttataggaa gtactggtag tggtaagtca actcttataa atttaattcc tcgtttcttt
     3481 gatgttactg aaggagaaat attacttggt ggagttaata taaaaaatgt ttctcaacat
     3541 gatttaaggg aaaaaattgg atatgtacct caaaagggag tattgttttc tggtactata
     3601 gaatccaact taaaatatgg tagaaaagac gcaacagatg aagaaatgag aacagcagct
     3661 gaaatagctc aagctacaga atttatagat tctaaaccgg aagcttttaa aactgaaatt
     3721 tctcaaggtg gaacaaatgt atctggaggt caaaaacaaa gactttctat tgcaagagca
     3781 atagcaaaaa atccagaagt ttatatattt gatgatagtt tttcagcact tgacttaaaa
     3841 actgatgcag ctcttcgtaa ggctttaaaa tcaaaaactg ctgaaagtac agttctaata
     3901 gttgcacaaa gaattagtac tatcttaaat gcagaacaga taattgtttt aaatgagggc
     3961 gaaatagttg gaataggaac acataaagag cttctaaaaa attgcgaagt ttatgagcaa
     4021 atagctttat cacaactttc aaaggaggag ctagctaatg agtagaatgg gaagaggtcc
     4081 tatgggaaaa tctatgggag caggacaaaa agcaaatgac tttaaaggga ctatgcgaaa
     4141 actaattgca tatttatcta aatttaaaat atcaataatt ctagtaattg tatttgctat
     4201 tggtagtgcg tcgttttcta tagtaggtcc aaaaatttta ggaaaggcaa ctacaaagat
     4261 atttgaaggg ctagtgagta aagtttctgg aggaaacgta ggtatagatt ttaatgcaat
     4321 tggaaaaatc ttaacctttt tacttttcct ttatttgata agtgcactgt tctcttttat
     4381 acaaggattt ataatgagtg gaatttctca aaaagtatcc tataatttaa gaaaagagat
     4441 ttcagcaaaa ttggatagat tgccaatgaa gtattttgat actaagacgc atggtgaaat
     4501 attatcgaga attacgaatg atatagatac tttaaatcaa agtttaaatc aaagtatgac
     4561 acagcttata acatcagtaa caactatgat aggtgttttg ataatgatgt tatctataag
     4621 tggaattatg acattagtgg cagttttaat attaccaata tcaatgtttg taatttcaag
     4681 aataataaag aaatcacaaa aatactttag atatcaacaa gaatatttag gaaatgtaaa
     4741 tggtcaagtt gaggaaactt acagtggcca aacaatagta aaagcattta atcgtgagga
     4801 tgaagtaatt gaagagtttg ataaactaaa tgatagcctg tataattcag cgtggaaatc
     4861 tcaattttta tcaggtataa tgcaaccact tatgatgttt attggaaact tgggatatgt
     4921 aatggtatca atcttaggag gttggcttgc aattaagaaa acaattgagg ttggtgacat
     4981 acagtcattt atacaatatg taagaaactt tacacagcca atgactcaga ttgcacaggt
     5041 tgccaattta ttgcaatcaa cagcagcagc ttctgaaaga gtatttgagt ttttagaaga
     5101 ggaagaagaa gtccaaattg ttgaaaatgc agtttcaata gatggtttag aaggtaaaat
     5161 tgattttgaa aatgtcaact ttggatataa cccaaacaag actataataa atgatttctc
     5221 agtaaatgtt gaacctggtc aaaaagttgc tattgttgga cctacaggag caggtaaaac
     5281 tacaatagtt aaactgttaa tgagatttta tgatgttaat agcggttcaa tactgattga
     5341 tggtcataat attaaagatt ttaatcgtag tgagcttaga gaaatgtttg gtatggttct
     5401 tcaagataca tggttattta gtggttcaat tatggaaaat ataagatatg gtaagttgaa
     5461 tgctacagat gaagaagtta tagaggctgc taaatctgca catgttcata gatttattaa
     5521 gacattacca gatggatata agatgaaatt aaatgaagaa gcaagtaatg tttctcaagg
     5581 tcaaaaacaa ttattgacta tagctcgtgc tattttagct gatcctaaaa ttttaatatt
     5641 agatgaagct acaagttctg tcgatactag aacagaagta ttgattcaaa aagctatgga
     5701 taatcttatg gaaggtagaa caagttttgt tatagcccac agattatcaa ctatacgtga
     5761 tgctgatatg attcttgtaa tgaatgaagg agatatagtt gaacaaggaa atcatgagga
     5821 acttctaaag aaaggcggat tctatgccaa tctttacaat tctcaatttg aagaagatga
     5881 agcaatgtaa caaaaaacta tcttagcata gacatgtaat taataagata aaataaatga
     5941 gggtgcctta aaatgaactt tttaattcat aaggcactct tttgtgtatg aaataaagtg
     6001 tatttttatc attttaacaa tgaaaaagag gattctctat attttgagat agccacataa
     6061 ttttatgtct ttacaaagtg tttattttat tataatatgt agtaaggaaa atgttggagg
     6121 tgattatcaa aatgtatatt taattttatt ctttaaattt tattataaat agaatttaat
     6181 tttaatagtt gatgattata ttgaagtata tgtttttatt acttttatta atcatgcaaa
     6241 taactactta aatttaagaa tagggttaaa tataaaatga taattacata taagttaatt
     6301 aaactaatgt atttttaata tatatttaga attttaatcc ttcttatgat ataagtagct
     6361 tgttttgtat tacttagatt tgtaaggagt ttttttattg aaaaaactgc ataatatatt
     6421 tgggaggagg tgtaagaaag tgatttttta tataagattg agaaaataat gcacgattat
     6481 ggttattata atagtaaata agaagtgtca tttaaggata agataaaaag caaaattctt
     6541 gatgaattta attatagagc cataatctcg tgctatagat taaataaaaa atagcagata
     6601 aggagaatat ggatatggcg aaaaaaggaa taacagtagg attaaacata aataataaat
     6661 ctgaggactt taatgaacta atgattgaac ttgaaaactt atgttcagca tgtgatattg
     6721 atgttgtagg gagtataact caaaatgcaa agcaggttaa tagagcattt tacattggga
     6781 caggtaaagt tgaggaaata ttaaacttaa taaagaaaga aaatatagag atagttatat
     6841 tttacaatga actttctaca tcacaactaa aaaatcttga agaaaagctt aattgtgaaa
     6901 ttatagatag aacggcattg atacttgata tatttgcaca aagggcaaaa actagagaag
     6961 ctaaacttca agtagaagta gcaagtttaa aatatatgtt accaagactt ataggttcaa
     7021 atgaaaattt aggacgtcaa agtggtggag ttggtactaa aaatagaggt tctggtgaga
     7081 aaaaattaga acttgaccgt agaagaatag aagaaaagat aacatcttta aatagggagt
     7141 tggatgattt aaaattccaa agagaaactc aaagaagtat gagaagaaaa tcaaatcttc
     7201 caaatgtagc tctagtcggg tatacaaatg ctggtaaatc ttcaataatg aataaactag
     7261 tagatatatt taaaaactct gaagaaaaga aagtgtttga gaaaaacatg ttatttgcta
     7321 cacttgaaac ttctgtcaga aatatagttc ttgcaaataa caaagaattt ttattatctg
     7381 atactgttgg ttttgtaagt aatctaccac atgacttagt aaaagcattt aggtcaacat
     7441 tagaagaagc atgtgaggca gacgtactgt tacatgttat tgatatttca aatccaagct
     7501 ataaaagtca tattaaagtg acagaagata ctttaaaaca aataggtgca gatggtatac
     7561 ctatgataca tgtgtataat aaaattgatt taatagatgt agacgtattg gatagaattt
     7621 tagatagtat tgataaagaa ggtatatttg tttcagttaa aaaagatata aatatagata
     7681 aaatgataaa atgtatttgt gatagcatat ttaaagatta tgtaagatgt aagttcttaa
     7741 ttccatatga taaaggtcat gtagtttctt attttaacga aaatatgagc attataaata
     7801 cagaatacag agaagatgga gctattttag atgtagaatg tagcaatata gaatataata
     7861 agtataaaaa gtatgcttta gagtaaattt tgaaaaactt attatagcct aaaatatatt
     7921 taatttataa tttattataa attgattgta aatatgattt tatgaaacgg tatagccata
     7981 atgctatgcc gttttgccat ttataataaa aataaattta ttgacataat agtaactaat
     8041 tactataata taaagtaatt agttactatt taaaattatc aaaaaattaa aatctaagat
     8101 aaagagaagt gtataataat ggatgcagaa actttaatga aaaaatatat ggtagatgat
     8161 gatagtagaa aacaagctat atttgcaagt atatttgtaa tgcaaaataa acttcaaact
     8221 acatgtgata aattagaccc agaattaaca atgaaacaat ggttactttt agctattgcg
     8281 gactctgtag atgaaactct tacattaacc aaacttggag aattaatggg gtgctcaaga
     8341 caaaatgtaa aaaaactagc tattgcttta gaaaagaaag gttttataca aattgagcag
     8401 aatgaaaaag attcaagagc tatctgtctt gtgacaaaag atagagtgca agagtattct
     8461 aaaaaagtaa gaggtatgca ggaaaaagta ttacagcttt tatttgaaga ttttacagag
     8521 aaagaagtgg aacagttatt ttttggaatt atgaaattaa gtataggaat tgaaaatgtg
     8581 aaaaattatg tagaaaataa tgacgttgga gaataagcaa gtatcaaatc aactttgtat
     8641 gacatcagag agggggataa tttatatgaa aaatgtagta gtgatttata agagtaagta
     8701 tggttcaaca aaaaaatatg ctaaatggct ttcagaatta ttgtcttgtg atatttttga
     8761 aactaagaat attaatatag aaaaggttat gaagtatgat acgattattt taggaggagg
     8821 tctttatgct tgtaatatag cgggaatttc tttcttaaaa aaaaattata aagaattgca
     8881 gaaaaaaaat ttagtagtat ttggcgtagg agctgctcca tatgatgatg aagtaataaa
     8941 gaaattaaga gaagtaaatt ttaaggatga attaaacaaa attccttgct tttattgtcg
     9001 tggggcatgg gatgagacaa aaatgtcttt tatagattat actctttgta gtttttttaa
     9061 aaaatctata gctaaaaaag aacgtcaagg tcttaaacca gcagaagcac ctttattgtc
     9121 tggagaagca tttgattggt gtaataaaga aaatttaact ccaatacttg attatataag
     9181 agaactttaa gaggtatata tttataatta tttattatct aaagttagat ttcatagaaa
     9241 cttactagac attatttata aaataatatt attaaagatg gataatttaa ttgattcaat
     9301 aaggttataa ttttaaaaaa tgtttatttt aaaataaatt attttataag cttacttata
     9361 cttaataagg ggcttttttt attggaagat ttatgttata ttgagatgaa tttattatga
     9421 tataaaaaat aatgttagga taaatttaaa tctttttcta aaaatgtatt aatataattc
     9481 ttaaagaaag gatttttaat aatggatata aaagagttag ttaaaatgca aagaaaatat
     9541 tataatacag gtaaaacaag agatatatcg tttcgtatag aacagttgaa gaaacttaag
     9601 ttagttgtat cacaaaatga agaaaaaata cttttagcac taaagaaaga tttaaataaa
     9661 tcggattttg aaggttttat gacagaagta ggcatgtttt atagtgaatt aaactttgct
     9721 ataaagaata taagaaaatg gagcaaaata aaaagagtta aatcaagtat ggttaatttt
     9781 ccatctatat ccaaagttgt tccacaacct tatggtgtta ctctcataat gtcaccttgg
     9841 aattatccat ttcaattagc acttatacca ttagtttggt cattagtagc agggaattgt
     9901 gttatattaa aaccttctga atattctgtg tcaacatcta gtgtagttaa agacatagtg
     9961 gaagatactt tttcaaaaga atatgttgca gttgttcaag gaagtcaaga agaaagtgaa
    10021 aagttactat tggaaagatt tgattatata ttttttacag gaagtactaa tgttggtaaa
    10081 ataatcatga aatcagcatc agagcattta actcctatta ctttagaact tggaggaaaa
    10141 agtccttgta tcattttaaa agatgctaat atagacctta ctgccaaaag acttacttgg
    10201 ggaaaactaa taaatgcagg acaaacctgc gtggctccag attatgtttt agtgcatgaa
    10261 gatagaaaaa atgagttaat agagaaaata aaatattata ctaataagta ttttggagat
    10321 aatccatgta ataatgagca atttcctaaa atcataaatc agagacattt taatcggata
    10381 ttatctctta ttgataaaga taagatagtc tatggtggaa attataataa ggagacatta
    10441 aagatagagc caactatagt agacaatgtt aattgggatg ataatataat gaaggaagaa
    10501 atatttgggc ctatatttcc cattttaaca tataaagatt tagatgaagt aatacaaaaa
    10561 attatacaaa tgccaaatcc attggcactt tatattttta caaaaaataa gtacctagaa
    10621 aataaacttt tagaaatgat tccagcaggc ggatgttgta tcaatgatac agttactcat
    10681 atagctacga attacttacc ttttggtggt attggtgaaa gtggtatggg ctcatatcat
    10741 ggaaaagctg gattcgatac atttactcac tataagagtg tgttaaaaaa attaaatttg
    10801 gatgtcccta ttagatatgc tccatacgat aataaattaa ttaaggttct taaaaagata
    10861 atgtaggtta tattgtagaa taataaatat aaataaaatt gagtatttgt attctgaata
    10921 tattttattt ttaatttggt atatttgtta taaaagtggt gattttgtga taaaatttaa
    10981 ttatatagaa ataaggagga aaaaatgaaa attaattgga atacaaaata cacaacaata
    11041 gcaatttata catttataat agctgctagt agtattatat tttatctagt atcatcacaa
    11101 attgatgtat tttctaataa tttagatgct atatttacaa cattacaacc ttttataata
    11161 ggatttgcta ttgcatatct tcttaatttt attttaaagt tttatgaaga cagaattttt
    11221 ataaaaagtg agaaattaaa aaaactaaaa caaagttcaa aaagaggctt gggtctttta
    11281 ttaacatatg caacagctac attgatactt tacttattta tgcatttcgt attgccacaa
    11341 gtaattgaga gtattgttgg actagcaaat gatataccta tgtatgtaaa caatgcaact
    11401 aagcttattg ataaattaat gactgattta aatcttgatg agcaatactt taatttggca
    11461 gttgataagt ggaatgagtt tgttacatat attataaagt ttgtaacaga tttaatccca
    11521 atattaggaa gtatgttaaa gaatgtagcc tctagtatat ggaatattgt actaggatta
    11581 attgtttcag tatacttatt aattgataaa gaaaaattct atggattaag caaaaaaata
    11641 acttatgcag tatttacaga aaaacaagct gccagaattt tagaattaac tcatagaagt
    11701 aactatacat ttggaagatt tttaggtggc aaaatattag attcattcat tataggtatt
    11761 ctaacatttg taatacttac gttagttaaa atgccatata cattattaat atcagttata
    11821 attggtatta caaatattat ccctttcttt ggacctttat ttggagctat accatctacg
    11881 ctaatagtga tgtttgtatc tcctataaaa gctttctggt tattgttgat tatattaata
    11941 atacaacaga tagatggaaa tattatagga cctaaaatac ttggagattc aattggtata
    12001 tctgcatttt ggattttgtt ctccttattg gttgcaggaa aattattagg ttttattgga
    12061 atggttatag gagttccaat gtttgcagta atttattcta taattaagga tattgttgaa
    12121 agtaaattgg ataaaaaggg attaccgaca gatacttccg attatatgta gctaaaaaat
    12181 attatgtata attaaaaata tataaattaa gcaatattta aagcactgag aaagttaaac
    12241 tcttggtgct ttttagatat aaaattatta tagaagaggt gttttttgtg gcatttatag
    12301 aatgtaaatt taaatctaaa tatactggag gagaaactga tataattgta attttgcctg
    12361 tagcaaatgg aaaagaactt tttagtggta aagagatata tgattttgaa ggtaaattta
    12421 aaacattata cttatttcat ggtttagaag atgaccaaag ctcatggatt agatatacta
    12481 atgtagagca ttatgctaaa aataaaaata tagccctagt aataccaaga gtagaaacta
    12541 gtttttatac cgatatggcg tatggacacc aatattttac ttttgtaagc gaggaattac
    12601 ctaaatttgt tagagctata tttcctttat cagataaaag ggaagataac tttgtagctg
    12661 gtatgtctat ggggggatat ggagctttaa aattagcgct ttcaaaacca aaagagtttt
    12721 cggcagttgc aagtttttct ggttcgcctg atatgattca tgagttagaa aatcaacaaa
    12781 tagaaaccaa tgcagacatt ctatttcacg attttggtag accagaagac gctagaaata
    12841 gtgagaatga tttactttac atacttaaga accttaaaga aagaaatgaa gatattccta
    12901 aaatttatca attttgtggt gatagagatt ttttatataa aaataatcag atgtttaaaa
    12961 aatatgcaga ttctctgggt gtagatgttg aatatgaaga gtgtgtaggg gggcatgaat
    13021 ggcgtctttg gaatttatgg ataaagaaat ttatagatat aattggatag tacacttatg
    13081 gtattaacag gtatactata aaatatcttt aaatatgata aagactgaaa tcctaaaatc
    13141 aaattatgat tttaaaattt cagtctctaa gaatttaata aaattttatt aagttcttac
    13201 acgagttatt attggagtaa aattgtcaac tatatctata tagtatttac tcaattcctc
    13261 taaaatctct tcgcataaat ccgtactatg accaagatta ataagaaact gttttgtaaa
    13321 atgctttatt acagcttgag aagatatatc gtacttgtgt gaagtataaa ctctaattat
    13381 ttcaggtttc atagcaagta taccaataca agaaagtatt aattcatcat gttctataga
    13441 ttctttatca ttaacatatc ttttcatgac catttcggtt acacttcttt gatagcttaa
    13501 aatagcttct tcataagcta tatccaaata aaaatttcta tatgcttcgt tttttagaag
    13561 gaaacaaagg tcagcattca tagcaagaaa gaacatcatc atagtacctt cttcatggta
    13621 gttatagatt aaagactgca tttcatttct tatatcttca tatattatag ctcctatttc
    13681 gcttttactt gagaagtaat agcttattag acctaaattt atattagctc tttctgcaat
    13741 ttgcctagtt gttgtcttat gataaccttg ctcaaaaaat aattctctag cagcacttat
    13801 aatactagct tttgtttctt tgcccttttc tgattttgac atatttactg tgtatacctc
    13861 tcttttcaat ctttttttga aaatttgcta actataaata ttgtaataga accacctatt
    13921 ataggaacca aaattctgtc aaatacagta agaataccaa tcatatttaa taatgccaat
    13981 ataacactta ctataacagc atagatagct cctttttttg tagcaccttt ccagcaaaga
    14041 ccaattacaa atggtgcaaa tattacacct gtacataatg gagctggcca aaacaataat
    14101 tctaataaac tatcaaattt aagtgctatg attgcaccta ttatagctgt caaagctgat
    14161 gcaatttgag aatactttag agtatttttt aaatcattaa attctatatt aggatgtaat
    14221 aatttgttat aaatatctct tgatataata gcactcattg aggtcattaa tgaatcggca
    14281 gtagacatta tggcagccaa tatagcagct aagaataata cagatattaa aggtggcaaa
    14341 gtagtcgata tcatttgagg taaaacctga acagatgata tatttggaaa taaactacca
    14401 ccaatagctc caattattcc tatagggaaa gcaagaaata atagtataaa tcctgcaatt
    14461 acactacttt taactgcatt tttagcatta tcagcacatt gatatcttaa aaaactttca
    14521 taagaaaata ttgagcctaa aactaatggt aaaactgtat acataaaatc actaatcgat
    14581 ttatttcctg gaaagaatgg ttcttttacc attgattgta acataagtaa agggttgtcg
    14641 ccaactaatt ttaagcttat ataagctact attggcaaaa acactataca aatagcaagt
    14701 tgaataaatg ctgatactgc aactccccac attcctgatg tataagtata tataatgata
    14761 actataactc ctataattac agctatatca taaggaatac caaatgcagt agatacagaa
    14821 ccaaaagaaa tcaattgact acccataata gctagtatag atattggtgt tacaagactt
    14881 tgataagctc ttaaaaatac actatgacca tatctagttt caatataatc agcaggtgtt
    14941 atatcacaag ataagcttct taattttcct gcgaaaaatg catttataaa gcaagctaaa
    15001 gctgcaccta tagtagaata cattccaacc caaagtcctc tttcagctcc ccattgaact
    15061 ccaccaacta agcaccctcc tccaaaagat gtagcagcaa tggttgcagc tagtataggc
    15121 aaaggcagat ttctacctgc gattaggaaa tcggatgctg ttttaacttt ttttgaaaaa
    15181 taaaaaccaa tccatatgta tattgcacac gtaacaatta aaagtaacat agtcttttct
    15241 cctttataaa atattttaat aaaatataca aaatatattt aaaatatatt ttatataaaa
    15301 gtagtacgat atttgtttta ttggttttag attaatatag ctataataat ttatgatatt
    15361 agatggaaat tacgtaaaaa tagaaaattg aaaattctga aatttttgta tattgatatt
    15421 tatctcatag attctttttt gtaattgtaa cacgctttta tttttaaatc aatagtacat
    15481 gtataaaaat aaaaggaatt taaataagaa aaagatattt ttaatatctg gatgtagtat
    15541 aga
//
LOCUS       DBGJFU010000090        15110 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.090, whole genome shotgun sequence.
ACCESSION   DBGJFU010000090 DBGJFU010000000
VERSION     DBGJFU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.090"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(126..830)
                     /locus_tag="ACOIAS_002637"
     CDS             complement(126..830)
                     /locus_tag="ACOIAS_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009587569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702339.1"
                     /translation="MLCLGLLNSCSGYSHDFNSSEEAQKYILSKLKDKYNEEFIITEV
                     KKYKEEKIGLNWIMAEVSSKENSSQTATVYTRNTGLFEDSYHVYYYFDEIKELATPLF
                     QDKPFIRDYQIEVQGHTATTEWNGKESVEEYLKKREYEIETSIYLNEGKTDEEYAEEI
                     SYIMQEIVESDLVFNISVYTNDNNLIFYSLPEQHSQPEVEVILEKMEDVKRQQETKKD
                     FKEWKKQNLNNETNSD"
     gene            complement(915..1244)
                     /locus_tag="ACOIAS_002638"
     CDS             complement(915..1244)
                     /locus_tag="ACOIAS_002638"
                     /inference="COORDINATES: protein motif:HMM:NF025461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4274 domain-containing protein"
                     /protein_id="HGZ2702340.1"
                     /translation="MIYNDLRSKLNEYNWDDGFEIPKQILADSSCDLALALEIFYLSD
                     GYAFLDDSTKTADLKEWGRFITVLYDDILNNKFPKTSTAFEIPLSQVQKYKLQKKDIS
                     KIFLTDL"
     gene            complement(1297..2070)
                     /locus_tag="ACOIAS_002639"
     CDS             complement(1297..2070)
                     /locus_tag="ACOIAS_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702341.1"
                     /translation="MNGQMFQIASIVATSKKALQLSEPIRFSQLEYENKIEFVFLPQK
                     KFLRTEKYTASNVSLWFEQIKKNGIQDIKLLCPYSVKDRQFLGFSNTTESSILCFYKS
                     GKVTYFTADWQFDSMQKKWNILYSEHEWTNPPSKKPYFENNINSFRDVLLSIKELAKK
                     IECENFANIFTSAINLLDGCSEYPDEKYGLSLPPIPQQNLQMFEAASISDVFGAMGSW
                     NDSPAYMAHKKGLSEEYETLSSELLKNVRLAILYAINEW"
     gene            complement(2351..3022)
                     /locus_tag="ACOIAS_002640"
     CDS             complement(2351..3022)
                     /locus_tag="ACOIAS_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2702342.1"
                     /translation="MEHYEKKISFLGENIQTIRKHRGMKQQELADKIGINMQSLSKIE
                     RGVNYPTFDTLEKIMDVLGVTPNELLSGEWKYIDHTEPYIMDIIKREQDFNVSLDYLS
                     ENEFFDNENEYRFYMETILIRYISNYLSNEVVEIEELMEIKQLIQRQKIERMMKVHKE
                     MRGLDRYREQPKEYKYHNPYDDWIFRQLADIDNRKNIPNTSPQVDFNEGDYEDYLKAK
                     WNRGL"
     gene            3519..4580
                     /locus_tag="ACOIAS_002641"
     CDS             3519..4580
                     /locus_tag="ACOIAS_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HGZ2702343.1"
                     /translation="MDYISIVFDTATAEDVIMHILGLPTDIFNVYPASIKYKTYQARW
                     QIGDIYVSGDARKTEDNPQGLGCYLVMTGRGCDDIFRILDSRNYTFGDMFRRCERRYG
                     LDNFHFTRLDIAIDDKNEKPFFTIEQIKKKCEKEEFISNSEGYHFDESKFDDFDTAKT
                     VYIGAGKSGLSYRFYDKDKEVCSKHNKTLEEVGSWKRTEMQLRDDKAHAFAMTFKDRP
                     LELGELAFGLLANNLRFVVPNRNESNKSRWKTCRFWERFLGAVEVLKLQVPKQQNSLE
                     ETQQWLTEGGVISAVKSFYFLEEHDALGGLEKVGTMLDKARYSNSLSSKLTAHLQRIN
                     RTDLIPYIQYDTKHGKGGI"
     gene            4580..4783
                     /locus_tag="ACOIAS_002642"
     CDS             4580..4783
                     /locus_tag="ACOIAS_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2702344.1"
                     /translation="MKNNDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWLI
                     MNGNRIQIKRRQFEQVIDKLDAI"
     gene            4862..6055
                     /locus_tag="ACOIAS_002643"
     CDS             4862..6055
                     /locus_tag="ACOIAS_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2702345.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     MKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQVLLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGKPKVAINYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            6301..6576
                     /locus_tag="ACOIAS_002644"
     CDS             6301..6576
                     /locus_tag="ACOIAS_002644"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2702346.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            6831..8531
                     /locus_tag="ACOIAS_002645"
     CDS             6831..8531
                     /locus_tag="ACOIAS_002645"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702347.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            8894..10081
                     /locus_tag="ACOIAS_002646"
     CDS             8894..10081
                     /locus_tag="ACOIAS_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702348.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            10141..11304
                     /locus_tag="ACOIAS_002647"
     CDS             10141..11304
                     /locus_tag="ACOIAS_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2702349.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            11455..12258
                     /gene="zupT"
                     /locus_tag="ACOIAS_002648"
     CDS             11455..12258
                     /gene="zupT"
                     /locus_tag="ACOIAS_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGZ2702350.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            12363..12914
                     /locus_tag="ACOIAS_002649"
     CDS             12363..12914
                     /locus_tag="ACOIAS_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HGZ2702351.1"
                     /translation="MEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGIFYF
                     HKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYKDIP
                     PKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVLQII
                     LLILYAVQLYRRLMYYLRLKKFK"
     gene            13164..13877
                     /gene="rgbR"
                     /locus_tag="ACOIAS_002650"
     CDS             13164..13877
                     /gene="rgbR"
                     /locus_tag="ACOIAS_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HGZ2702352.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            13871..>15110
                     /locus_tag="ACOIAS_002651"
     CDS             13871..>15110
                     /locus_tag="ACOIAS_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HGZ2702353.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNKYIRIKGTMTRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKF"
BASE COUNT         5447 a         2057 c         2658 g         4948 t
ORIGIN      
        1 tgtttaatgt cttgttcttt tagcatttta attgtcctcc tataaaatta tttttcgcat
       61 aacttactgt tttcatttta tcatttcaat atagtgatta caatgtattt cgtgaaatta
      121 caacatcaat cagaatttgt ttcattgttc agattttgtt ttttccattc cttaaaatct
      181 ttttttgttt cttgttgtct tttaacatct tccatttttt ccaaaatcac ttctacctct
      241 ggttgactgt gctgttcggg tagagaatag aaaataaggt tattatcgtt tgtatatact
      301 gaaatattaa aaaccaaatc actttctaca atttcttgca tgatatagga aatttcttct
      361 gcatattctt cgtctgtttt tccctcgtta agatatatac ttgtttctat ttcgtattct
      421 ctttttttca gatattcttc tacactttcc ttaccattcc attctgttgt ggctgtatgc
      481 ccttgaactt caatttgata atctcttata aaaggcttat cttgaaataa aggagttgca
      541 agttctttta tttcatcaaa atagtaataa acatggtaac tgtcctcaaa aagtccagta
      601 tttcgtgtat atacagtagc agtttgagaa gaattttctt tactggaaac ttctgccata
      661 atccagttta atcctatttt ttcttctttg tacttcttca cttctgtaat gataaattct
      721 tcattgtatt tgtcctttaa tttggataag atatattttt gtgcttcttc actagagtta
      781 aaatcatgcg aataaccgct acaactattt aatagtccta gacaaagcac gcttgctata
      841 acacctaata aaattttttt atatcgcata atcgccacct caatgtgatt taatacaatt
      901 ctacaaactg atagttataa gtcagtcaaa aaaatttttg aaatatcttt tttctgaagt
      961 ttgtattttt gaacttggga taatggaatt tcaaatgctg tgcttgtctt tggaaattta
     1021 ttgttcaata tatcatcata gagaactgta ataaatctcc cccattcttt taagtcagct
     1081 gtctttgttg aatcgtctaa gaaagcataa ccatcactta aataaaatat ctctaatgct
     1141 agtgctaaat cacaactgga gtcagcaagt atttgttttg gaatttcaaa tccgtcatcc
     1201 cagttatatt catttagctt acttctcaaa tcattatata tcatttttat attccccaaa
     1261 ttctaatttg catgtatgaa atataggatt ataatcttac cattcattta tggcatacaa
     1321 aatagcaagt cttacatttt ttaacaactc agaagataga gtttcgtatt cttcagataa
     1381 cccttttttg tgagccatat atgctgggct atcattccac gaacccattg ctccaaaaac
     1441 atccgaaata cttgcagctt cgaacatttg aagattttgc tgtggaattg gtggtaaact
     1501 caaaccatac ttttcatctg gatattcgct acagccatct aaaaggttta tagcagaagt
     1561 aaaaatatta gcaaaatttt cacactcaat ttttttagcc aattctttaa tcgataataa
     1621 tacatctcga aatgagttaa tgttattctc aaaatatggt ttttttgatg gtgggttagt
     1681 ccactcatgc tctgaatata gaatattcca ttttttttgc atagagtcaa attgccaatc
     1741 agcagtaaag tatgttactt ttccgctttt gtaaaaacac aaaatggaac tttctgttgt
     1801 atttgaaaat cctaaaaatt gtctatcctt tactgaatat ggacatagca actttatatc
     1861 ctgtatccca tttttcttta tttgttcaaa ccacaatgaa acattagaag cagtatattt
     1921 ttctgtcctt aaaaatttct tttgaggtaa aaagacaaat tcaattttat tctcatattc
     1981 taattgtgaa aatctaattg gttctgataa ttgcaacgct tttttgctcg tcgctacaat
     2041 agatgcaatt tgaaacattt gtccattcat catttcgcct ccccttaata tgaataatac
     2101 tgttctttca aaaattctaa tttgtcgctt ttttcatatc catattataa cagttccata
     2161 tatctgccac aataaaaatc aacataccct ttaaatattt tctgcattga acatacacac
     2221 tttttctttc ttcctcttga ttacccacca cagaaaagaa aaagccctgt caagagcctt
     2281 gccaccattg gtggtgcttt gcactcttta ctgggctttt tgtttgctgt atcttccatg
     2341 caagaggaaa ttaaagacct ctgttccatt tcgcctttag ataatcttca taatctccct
     2401 cattaaagtc tacttgtgga gaagtattag gaatattttt tctgttatct atatctgcaa
     2461 gttgacggaa tatccagtca tcataagggt tgtgatattt gtattctttc ggctgttctc
     2521 tgtatcggtc taaccctcgc atttccttat gtactttcat catgcgttct attttttgac
     2581 gctgtatcaa ctgtttgatt tccataagtt cttcaatttc tacaacctca ttagaaagat
     2641 aattgctgat gtatctaatc aatatggttt ccatgtaaaa tctgtattcg ttttcattat
     2701 cgaaaaattc attttcagac agataatcta aagaaacatt gaagtcctgt tcccgtttga
     2761 taatatccat gatatatggc tcggtgtggt cgatatactt ccattctccc gacaataatt
     2821 cattgggcgt tactcccagc acgtccatta tcttttccag cgtatcaaag gtggggtaat
     2881 tcacacctcg ttcaatcttg gaaaggctct gcatattgat accgattttg tccgccagtt
     2941 cctgttgttt cattcctctg tgttttctta tggtctgtat gttttctcct aagaaactga
     3001 ttttcttttc gtaatgctcc atgacttagt ataaacactc ctttcattgc ttttcttggt
     3061 atttataagc atatcatact taatatatat caaaaagtca acaaaaaatt cttgacaagt
     3121 aattctaatg gcgttattat cgtatcagat agagtgatta agcacaatag ggtcaatcac
     3181 acgcttgagt aagcatagaa aagatgtgtt ttcattttga tagcatgaat aagcatggac
     3241 tatcagaccg aaaataaggt ttctgactgg ggctgttccg tttagccacg agccttacaa
     3301 ggctcgtgag cgttaagaac ggatcagaca caggaagaaa aggaacgcct ttaggcgttc
     3361 ataaagggcg tatatgtaac accgccctgc gcatacatgt catagtacct agaaactgtg
     3421 ataaataaag gaaaaaacac gatttttacc ccgcaaaaaa acgggaagta cgaaaggaga
     3481 aatgcactat aactacacac aaaaatttat ccgttaagat tgattacatc agtattgtat
     3541 ttgatactgc aaccgctgaa gatgtaatca tgcacatttt aggtttaccg actgacattt
     3601 tcaatgtcta tccggcaagc attaaataca aaacctatca agcacgctgg cagattggag
     3661 atatttatgt atcgggggac gcaagaaaaa cagaggataa cccacaggga ctaggctgtt
     3721 atcttgttat gactggcaga ggttgtgatg atattttccg tattctcgac agtaggaatt
     3781 atacctttgg ggatatgttc cgacgctgtg agcgaagata cggactggat aacttccact
     3841 tcacaaggct tgatattgcc attgatgata agaacgaaaa gccattcttt accatagagc
     3901 agataaagaa gaaatgcgaa aaagaggaat ttatctccaa tagtgagggc taccactttg
     3961 atgaaagcaa gtttgacgat ttcgacaccg caaagactgt ttatatcggt gctggaaaat
     4021 ctggattatc ctaccgcttt tatgataagg ataaggaagt ctgttcaaaa cataataaga
     4081 cgcttgagga agtcggcagt tggaaacgga cagaaatgca actgcgtgat gataaggctc
     4141 atgcctttgc catgacattt aaagacagac cgctagaact tggggaactg gctttcgggc
     4201 tattggcaaa caacctacgc tttgtcgtgc caaacagaaa cgaaagtaat aaaagcaggt
     4261 ggaaaacgtg tcgattttgg gaacgctttt taggggctgt ggaagtcttg aaactgcaag
     4321 taccaaaaca gcaaaattcc cttgaggaaa cacagcaatg gctcacagag ggtggcgtga
     4381 tttccgctgt caaaagtttt tatttcttag aagaacatga cgcattaggt ggactggaaa
     4441 aagtaggaac tatgcttgat aaggcaagat acagcaattc cctttccagt aaattaaccg
     4501 cccatttaca gaggataaac cgcaccgacc ttatccccta tatccagtat gacacgaaac
     4561 atgggaaagg gggtatctga tgaagaacaa cgatattccc gtatgggaaa aatacaccct
     4621 taccattgaa gaagcgtcaa agtatttccg tatcggagaa aacaagttaa gacgattggc
     4681 agaggaaaac aaggacgctg gctggctcat tatgaatggc aaccgcatac agattaaacg
     4741 ccgacaattt gaacaggtta ttgataaatt ggacgcaatc taatgcaaat gagccttgta
     4801 tgtgttatga tgaacacaag tcatatcaag gctctttcca acaaggaaag gagcagacac
     4861 catgaaagaa aaaagacggg ataacaaagg tcgtatcctg catactggag agagccaacg
     4921 aacagacggg aaatacttat ataaatatgt ggacgcattt ggaaacacaa aatatgtgta
     4981 tgcttggaga ttgacaccca cagacccgac accaaaggga aaacgggaaa aaccctcact
     5041 tcgtgaactg gaacagcaga taagacggga tattgaggac ggtatcgaca gcacaggcaa
     5101 gaaaatgacg ctttgccaac tttacgccaa acagaacgca cagagggcaa acgtaaagaa
     5161 aagcacaatg aaacaacggg aacaactcat gcggttattg aaagaggata agttaggtgc
     5221 taggagcatt gatacaatca agccctctga cgctaaagaa tgggcgttac gcatgaaaga
     5281 caaaggcttt tcctacaata ccattaacaa tcataaacgc tcgttaaaag cgtcattcta
     5341 tatcgccata caagacgatt gtgtaaggaa aaatcccttt gatttcaagt taagtgaagt
     5401 cctagaaaat gataccaaag agaaagtcgc attgacagag gaacaggaac aagtcctact
     5461 ctcatttatc aagacggaca atgtgtatca taagcattat gatgatgtgc taatattatt
     5521 aaagacagga cttcgtatct cggaactgtg cggactgaca gtagccgata ttgatttcaa
     5581 gaatgaagtt gtgattatcg accaccagtt actaaagagc aaggaacagg gctattatat
     5641 tgaaacgcct aaaacaaaga gcggaataag gcaagtgcca ttaagcagag aaacgataca
     5701 ggcatttcaa cgggttatga agaaacgccc aaaggcagaa ccatttgtga tagacggacg
     5761 gagcaatttt ctgtttgtca atcataaagg caagcccaaa gttgcgatta attataacgc
     5821 cttatttatc cgtatggtaa agaaatacaa caaacaccac aaggacaatc ccttgccaca
     5881 tatcacaccg catacgctac gccatacatt ctgcacaagg ctggcaagca agaacatgaa
     5941 cccgaaagat ttacagtata tcatgggaca ttcaaacatc agtatcacaa tgaactggta
     6001 cgctcatgcg tccatagaca ccgcaaaatc agaggttcag cgtctaatcg catagaagta
     6061 tttaccacga ttttaaccac gattgatagg gaaaatataa gaagatagac ctagatatgt
     6121 gaggtttacc acaaaagaaa aatgcccgta gagccgataa aataaggctt tgcggacatt
     6181 taagaagata taaaaagata gtcaaaaaga catatataat ttctataaat aaagatataa
     6241 gaaatttatc aattttattt tgagttataa aatattaaag aaattgtagg aggacaatat
     6301 atgaaaaaaa tattagtagc ctgtggagca ggaatcgcaa catcaacaat agtttgtgac
     6361 agagtagaaa gattggttaa agaaaataat atacaagcag aagttgagca atgtaaaatt
     6421 gctgaatgtt caacaaaaca agaaggtgct gatttaatcg tatctactac tatactacca
     6481 acaacttatg atatacctac aataaaggca acaggatata taacaggtat aaatacaaca
     6541 gcattagata aaaagatatt aaatgcattg aagtagtttt gtatttataa aaaaaattta
     6601 gtattaaata tagagttgta tctttataaa attttaaata tagagatata gctcttttta
     6661 tgttgtttca agtatacaac tatatgatat aaatcttcaa ttttatgaaa aagattgtta
     6721 ttactttgta agtttataaa agatttaaag ttattgttat gttatactaa tttatgggta
     6781 ttttttagaa gtactaatag aagtactaat aaaggaggga tttaatactt atgataaaac
     6841 aatttgtcaa atattataag ccatataaga aaatttttac actagacctt atagcagctt
     6901 ttctattttc cttatgtgat ttagtttatc ctatgataac tagaaatata atggatgacg
     6961 ttgttccaaa taaaaactta agaatgttag tagtttttgc agtagcactt atacttatat
     7021 ttatagcaaa agcagggctt aactatttta tgcagtattg gggacatgtt attggtgtag
     7081 atatgcaagc tgatatgaga aatgaggtat ttacacactt acaaagactt ccaaatacgt
     7141 attttgataa taacaaatct ggagtaacta tgtctagaat agtaaatgat ttaatggata
     7201 taacagaact tgcacatcat gggcctgaag atttattcat ttctatagta atgttggttg
     7261 gttcattctt tatacttata gatataaata ttcctcttac gcttataata tttgctatat
     7321 taccgtttat aatttggttt gcaatagcta aaaaagataa gatgaatatt gcttttatga
     7381 agagtagagt tactataggt gatgtaaatg ctactttaga aaatagtata gcaggtatga
     7441 aggtaactaa gtctttttgt actgagaaag aagagttaaa taagtttgta agaagcaata
     7501 aattatttag aagagcaaga caagattctt ataaggtaat ggcagaatat tactctggta
     7561 tgaatttata catggatatc ttagagtggg tagttgtaat agctggtggt tattttactt
     7621 atataggaaa gattactttg ggtgattttg ctgcttatat actatatgta aaaatgttta
     7681 tacaacctat gaaaaaatta ataaacttta cagagcaata tcaaaatggt atgacaggtt
     7741 ttaaaagatt catagagata atggaacaag accatcaaaa agaagctaaa aatcctattg
     7801 aacttgaaaa tgtaaaaggt gacattgaga tagaaaatat atctttcaca tatgaagata
     7861 agactcaagt tttggataat ttaagtttat ctataaaagc tggtaagact atagcattag
     7921 ttggaccttc tggaggagga aagactacat tatgtaattt attgccaaga ttttatgaat
     7981 ttgataaggg tgatataaaa attgatggaa agagtataaa agatgttagt ttaaaatcat
     8041 tgagaaaaaa tataggtata gttcaacaag atgtattttt atttacagga actataagag
     8101 ataatattct ttgtgggaac cctaatgcca cagatgaaga aatgatagct gctgctaaaa
     8161 aagctagaat acatgacttt gtagagactt tgccagatgg atatgatact tatataggag
     8221 aaagaggcgt aaaattatca ggtgggcaaa aacaaagaat ttcaatttca agaatctttt
     8281 taaagaatcc tccaataata atattggatg aggctacatc tgctttagac aatgtaacag
     8341 aaagagaaat tcaagagtct ttagaggaat taagtaaaga tagaacaaat ttagttgttg
     8401 cacatagatt gactactata aaaaatgctg atgaaatcat agtattgacg gataaaggaa
     8461 ttgaagaaag aggtactcat gaagagctag taaacaaaaa tggagtatat agtaggttac
     8521 acaataacta gtatacaaat tttacaccat agctaacata cacattaaac ttacaaacat
     8581 aggatatatt aagattcata aagacaattc attttatatt tataagatgt tttatgaata
     8641 aaatgtatcc tatgttttat tttttgcaaa ttacatttaa ttctctttgt aagaatagag
     8701 ttaaatagcc aatggtcggg ataacgtttt tacaaaaaga ataatttaga aaactaactt
     8761 taatttatgg aatttatcga attttatgaa aaaaatcgaa ttttgacttg aaatgttttt
     8821 caaaagtagt aaaataatgg ataactatac atacatttta taaaaatata tttattatga
     8881 ggggggattt tgtatggagg caataacagc tttttgtcta gttttagtat cactgacgat
     8941 aggagatata gtttcagcaa aaactaaagc ttttgttccg tcggtttttg tatcagcaat
     9001 tatatttata gtaggatttt ggactttttt cccagaaaat attgtggact tagccgcttt
     9061 aggaacacca ttagctcaat taggtatgct tttgttaata actcatatgg gaactatgat
     9121 gagtattaag gaattagcag gtcaatggaa aacaatagta atagccttgg caggaatagt
     9181 aggaatatgt gtaggtgctt tggcattagg gacagtagta tttggatggg atacagcagt
     9241 aatagctaca cctcctctta ctggagggct agttgcatct ataatgatgg cagatgctgc
     9301 tacagcaaaa ggtcttgcaa gtttatcagt acttgcaatt ttgatgtatg ttgcacaagg
     9361 atttgcgggt tatcctatta cagcattaat gcttaaaaaa gaaggaaaaa gacttttatc
     9421 agactttaga agtggaaaag taactgtaaa tacagatgaa gaaaaagtta aggatttacc
     9481 tgaacaaaaa agcagattta agataatacc agacttacca gaaaaatatg atacgacata
     9541 tatgataata cttagattag gatttgtagc atggttagca gtacaattta caaatcttac
     9601 aaatgaagtt gtatcaagat atgttatatg tttaatattt ggtgtaatag catcagaact
     9661 tggaatagtt gatagaaaac cacttaataa atctggttca tttggatggt taatgacatc
     9721 attgatggct tttatatttg cagggttagc aaaagctaca ccagcaatgc ttgttgaaat
     9781 ggcagttcct cttgtaggaa tcataatatt tggtgtaata ggtatgggaa tattctctat
     9841 tatagtaggt aagcttttag gtgaaagtaa agaaatggct ttttcagttt cgcttacatc
     9901 actttatggt tttccaccaa actatatatt aactgaagag gcagtaaagg ctttggctga
     9961 aacaccagaa gaaaaagaat ttttaatgga taagatgctt ccaaagatgc ttgttggagg
    10021 atttactaca gttacaatag cttctgtagt aatagcaggt atctttgtaa atctaatata
    10081 gtatttaagt gataaaaagt attttaaata agaagaatgt gtactacttg gaggtatgga
    10141 atggatatta aagaaataac aaagagttat aaagactatg taattaagct tagaagggaa
    10201 tttcatgaaa atcctgagaa aagtatggaa gaagtgagaa catcaaagag agttaaagaa
    10261 gaacttgata aaatagggat accatatgta tcagctggag gtacaggtgt aattgcaact
    10321 ataaaaggag ctaatccagg aaagactgta gcacttaggg gtgatatgga tgctctacaa
    10381 gttgttgaat gcactgatgt agaatacaaa tctaaaaatg aaggacttat gcatgcttgt
    10441 ggtcatgatg gtcacacatc aatgcttcta ggggcagcaa aagtgttaaa tgatattaag
    10501 gatagtatca atggaactgt aaaattattt tttcagccag gagaagaagt tggtaaaggt
    10561 gcaagagcta tgattcaaga tggtgctatg gaaggtgtag atagtgtgtt tggaatacat
    10621 ctttggactg atgtagaatc tgggaccata tcggtagaag aaggaccaag aatggcttct
    10681 gctgatttct ttaaaattac tgtaaaaggt agaggtggtc atggttcact tcctcatcaa
    10741 ggtgttgatg cagtgcttgc aagttcagca atagttatga atctacaatc tatggttagt
    10801 agagaagtaa gtcctctgga accattagtt gtgagtgttg gagttttaaa ttctggaact
    10861 agatttaacg taattgcgag tgaagctgtc ttagaaggta caataaggct atttaatcca
    10921 gagcttagaa agcaaattcc tggaatttta gaacgtatag caaaatctac agcagaagca
    10981 tatagagcag atgctgaatt agagtatggg tacttaacac cagcagttat aaatgacaaa
    11041 gaatgttcaa agatagctac agatgcagca attaagctat ttggagaaga ctgtataaca
    11101 ctatttgaaa aagtaacagg agcagaagat ttagctgagt ttatgaatat agcacctggt
    11161 gcactagcat ttgtaggagc tagaaatgag tcaaaaggtg cttgttatcc tcatcatcat
    11221 ggatgcttta atatagatga agatgctcta gaaattggaa ctgctttata tgtgcaatat
    11281 gcagtggatt tcttaaataa ataattttaa tatactataa agaaaaagca gagactaagt
    11341 ttatatctct gcttttttat tgtgtatatt ttttataaat attgactata taaaatatat
    11401 atgatagcat aaagaaaatg ataatcaata tcaattaaaa agaaggtgat ttatttgaat
    11461 aatgtaatat ttgcattttt gataacatta ttggctggtt tatctactgg tataggaagt
    11521 tgtatagcat tttttgctaa aaaaacaaat actaagtttc tttctataag cttaggattt
    11581 tctgctggtg ttatgattta tgtttctatg atagaaattt ttcctaaagc acaagatgct
    11641 ttgacaaaat ctatgggaga gaaattagga agttggtcaa ctgtaatagc tttttttata
    11701 ggtatggcta tcattgctgt tattgataag ctaattccac aagaagaaaa tccacatgaa
    11761 ataaagaaaa tggagaatat agaagaaaag aatatcaaaa aaaataagtc tcttttgaga
    11821 acaggtatat ttacagcaat ggctatagca attcacaatt ttcctgaagg attagctaca
    11881 tttatatcag ctcttgatga tgttacaata gctattccga tagcaattgc aattgcaata
    11941 cacaatatac cagaaggtat atctgtatct gtaccagttt attatgccac aggagataaa
    12001 aagaaagctt tttattattc ctttttatct ggtatgtctg agcctttagg agcgattatt
    12061 ggatatgtac ttcttagaaa ttttttaaat gatataactc ttggaattgt atttgcaatt
    12121 gtaggaggaa ttatggtatt catatcacta gatgaattat tgccatcagc tagagagtac
    12181 ggggaacatc atttatctat ttatggattg attgcaggaa tgggtgttat ggctattagt
    12241 ttattactat ttaaatagct ttttaaatat atgattgtaa cctaaaatta gtatcaaaat
    12301 gatataatta tttaaataga tttttaagct atttaaaaat atcattttgg agaatgagtg
    12361 ctatggaaaa gaaaataaaa ttcaaatttg actggtatag ttcacctgat aagatagaga
    12421 aatggttaga agatattgaa gctaaaggat ataatttata taagataaat aaatttggag
    12481 gaatatttta ttttcataag ggaagtccaa gaaaaataaa atacactgtt gattactact
    12541 atgatgtaga gaataattat tttgatgaac ataaatcaaa tggctggaat cttgtattta
    12601 ggagtattgg tagttttatg ggtacatctg catggtatat atggtcgaaa gaatacaaag
    12661 acatacctcc taaattatat agtgattctg ataaaaaaaa caaagtaaag tcaattttat
    12721 ttatgaatat gtttatattc tttatgttag tagtaatatt tatatattta ttgaatattg
    12781 aaataagaaa tttatatagt ggttatggag catctgtaac tgctattgta ctacagatta
    12841 tattgttgat tttatatgca gtacaattat ataggagatt aatgtattat ttaaggctta
    12901 aaaagtttaa ataaataaaa gaaggtgtcc taaaattatt tttttaatag tttaagacat
    12961 cttcttttat cagtaagtac tgtttttaat tatagtaatt tctgtcttta aattgaaata
    13021 tcaaataata gttatatggg attgagtatt gataaaaata atttgaaaat gttatcctaa
    13081 agtatcagat atacaaaatt atctttaaat atattataaa tattttaatt gtattaacta
    13141 gaaattatgt aaggaggcat tttatgatta agatagcagt ttgtgaagat gaaaaagaaa
    13201 cacaactttt aatagaagat taccttgaga atatattaaa agatataagt atagaatatg
    13261 agatacaaaa gtatatatct ggagaagagt tactggaaag taatttaaaa gatatagata
    13321 tattacttct tgatataaaa atggaaaaat taaatggaat ggatactgca agaaagatta
    13381 gagaagtaga caatgaaatg gaaataatat ttgtaacttc attaatagat tatgtgcaag
    13441 aaggttatga ggtaagagct tataggtatt tactaaagcc aatagagttg gaagaactaa
    13501 aaaaacatgt gctaacttgt atcaaagata ttgaaataaa taaggaaagt catattacaa
    13561 taaaaaataa gtctaataca tataagattt atttaaatga aataaaatat atagaagttc
    13621 aaaaaaaaga tatgctaata cacacaataa acaagaattt tgatataaaa tatagtttag
    13681 gcaaaataga aaaagaatta aatccatata aatttataag atgccataaa agttttatag
    13741 taaatttaag atatgttgaa aatataaagc ctaatactgc aatactggaa agtggagaag
    13801 aggtacctat tagtagatat agatataaag aagttaagga gaaattctta aaatttcttg
    13861 gtgatacaat atgctaaact ttatgactga gctattagta gttttatcta tatttataca
    13921 gttttctata atgaacggaa ttttaattta taaaaaggat agtaaaaaaa atgaaaaaat
    13981 agtattctat attgtcttgt ttcttattta tattataatt cagataagta gattatttaa
    14041 tggaggaaat tactttagta atataaacta tttattttat atattagcat ttattgcttg
    14101 gggaggatac tataattatt tttataagat gggttatatt aagtattcca ttttctttta
    14161 tctatttagt gtatattata aatgtttgga tgctgttata tatagaatac tattttttgc
    14221 aattacaggt aatgttattt catctaaaga atccttatta gtacctcact tagaatctat
    14281 aaaaattcag atatatagta atttaataat tattcttata tatcttatat ttagcttttt
    14341 taggaagtct ataaaattaa ttagccaaga taagagaaat tatgtatatt tattatttgc
    14401 cttattagtt aatacaatga atatgcttgt aaattatgta ttaaaaaaat tagaacggtt
    14461 tgggtcatta cattcagaag gatactattt tgataatttt gttaatccaa aattggtagg
    14521 agctagtagt atatttctta tactattgtt taaagaaata ataaaagaga atagattaaa
    14581 atctcaagct gaattaataa aaaataagtt agatatgcaa tatgcgcatt atttaagtat
    14641 tcaagaatct catatgcgag ttaagaaact atatcacgat ataaataatc acatttattg
    14701 tatagacaac cttagaaaca atagcaaaga aattaatgaa tatgttaaca atttaaaaga
    14761 tgaaataaaa acatttaaat atatctataa tacaggaaat atgatattag acattataat
    14821 caacgaaaag agtgaaatat gtttaaaaaa aggaattaaa tttacttgct ctataaattt
    14881 ttcaaaagta aattttgtaa aacctataga tgtgtcaagc atattttcta atattttgga
    14941 taatgcaata gaagcatgtg ataaaatagt ggatgaaaat gttaataaat atattagaat
    15001 taaaggaact atgacaaggt cattttttgt acttaaatgt gaaaatagta aactaaatca
    15061 gcttactttt aataaaaata tattgttaac aaacaaaatg gataagtttg
//
LOCUS       DBGJFU010000091        15028 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.091, whole genome shotgun sequence.
ACCESSION   DBGJFU010000091 DBGJFU010000000
VERSION     DBGJFU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.091"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..798
                     /locus_tag="ACOIAS_002652"
     CDS             <1..798
                     /locus_tag="ACOIAS_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HGZ2702354.1"
                     /translation="HKKVFIDKSIIKEINFDIKNDSNSIEKIKYIEDVVRFVKEEIGK
                     SKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDILSMIEFEITQYIPININNYSIKYKV
                     INSKNDKLNIQVILFPKSIIQLIKNISENLNMNPKTININFDILQKLINLNLIENFSE
                     EGVFIECKKSQLIINITKDKKIRETYLLSKEGKSYEFIKRLLRDFKYVYYYGIENCFI
                     EEYFKYTDKSIEVNPLRLKDKVKVENNFDADLEDNRINYISNIGMII"
     gene            799..1242
                     /locus_tag="ACOIAS_002653"
     CDS             799..1242
                     /locus_tag="ACOIAS_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702355.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINIITKISIDK
                     NKLEIEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            1257..1841
                     /locus_tag="ACOIAS_002654"
     CDS             1257..1841
                     /locus_tag="ACOIAS_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702356.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANLLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            2094..3131
                     /locus_tag="ACOIAS_002655"
     CDS             2094..3131
                     /locus_tag="ACOIAS_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HGZ2702357.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            3212..3577
                     /locus_tag="ACOIAS_002656"
     CDS             3212..3577
                     /locus_tag="ACOIAS_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702358.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            3582..3899
                     /locus_tag="ACOIAS_002657"
     CDS             3582..3899
                     /locus_tag="ACOIAS_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702359.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3947..5551
                     /locus_tag="ACOIAS_002658"
     CDS             3947..5551
                     /locus_tag="ACOIAS_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGZ2702360.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            5675..7303
                     /locus_tag="ACOIAS_002659"
     CDS             5675..7303
                     /locus_tag="ACOIAS_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGZ2702361.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTSLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAALDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNIYFSEEGQSELD
                     VMYNRVVSAYTYALESMRSDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            7568..8917
                     /locus_tag="ACOIAS_002660"
     CDS             7568..8917
                     /locus_tag="ACOIAS_002660"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGZ2702362.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            9073..10152
                     /locus_tag="ACOIAS_002661"
     CDS             9073..10152
                     /locus_tag="ACOIAS_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HGZ2702363.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            10497..11405
                     /locus_tag="ACOIAS_002662"
     CDS             10497..11405
                     /locus_tag="ACOIAS_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase activase"
                     /protein_id="HGZ2702364.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            11410..13779
                     /gene="hypD"
                     /locus_tag="ACOIAS_002663"
     CDS             11410..13779
                     /gene="hypD"
                     /locus_tag="ACOIAS_002663"
                     /EC_number="4.2.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase"
                     /protein_id="HGZ2702365.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            14104..14907
                     /gene="proC"
                     /locus_tag="ACOIAS_002664"
     CDS             14104..14907
                     /gene="proC"
                     /locus_tag="ACOIAS_002664"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGZ2702366.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         6214 a         1519 c         2723 g         4572 t
ORIGIN      
        1 cataaaaaag tttttataga taaatcaatt ataaaagaga ttaactttga tataaaaaat
       61 gatagtaatt cgattgagaa gattaaatat atagaagatg tagttagatt tgtaaaagag
      121 gagattggta aatcaaaaat attatataaa aatttatact ttaacattca aattcaggat
      181 atagtcatta gaaatgttga agttctaaat actaagaaaa aaagagacat attaagcatg
      241 attgaatttg aaataacaca atacattcca ataaacataa ataactattc aataaaatat
      301 aaagttataa attctaaaaa tgataaattg aatatacaag taattctttt cccaaagagt
      361 attattcaat taattaagaa catttcagaa aatctaaata tgaatccaaa gactataaat
      421 ataaattttg atatattaca aaagcttata aacttaaatt taatagagaa tttttcagaa
      481 gaaggggtat ttatagaatg taaaaaaagt cagttaatta taaatataac aaaagataaa
      541 aaaataagag aaacatactt gctatcaaaa gagggaaaat catatgagtt cataaaaaga
      601 ttattaaggg attttaagta tgtttattac tatggtatag aaaattgttt tattgaagaa
      661 tactttaaat atacagataa atctattgaa gtaaatccat taagattaaa agataaagta
      721 aaagtagaaa ataattttga tgcagattta gaagacaata ggataaatta tataagtaat
      781 ataggaatga ttatttaagt gaaagtggat aaaacgataa attttcttaa tggatttaaa
      841 aaaactaaac catgtaataa aatctataaa ataataatag tcttaatttt aacgtgtatt
      901 ttatttaaat catttatgta tgtgaaatct ataaatgaac ttgaagatta tataagtcaa
      961 aataagctag tggataaatc aagacacact ttaccaacaa cagataaaac tagagagaaa
     1021 ttttctaaaa taaaacttaa tgaaataaaa aaaatatatg aaacaattgg aataaatatt
     1081 attactaaaa tatcgataga taaaaataag ctagagattg aaggtgtgtg tagtgattta
     1141 gatgttttaa acaatctaag gaaaataaag tacataaaat attattacat agacagtatt
     1201 gtaagagatg gagaatatta tagatttaga ataaaaagtt agatgggtga ttatttttga
     1261 gattaaataa tagagaaaaa aatctactta aaatattgtt aatattagtt attatattgg
     1321 caagtgatat agcagtaagt tatttggaag aaaacttaac tagtttgaag aatgaaaagc
     1381 aaaatataat aaataaaata aataatcaag ataattatca aataaaggat aataagcata
     1441 tagatgataa atatatagaa agtgttgaat caaataaaaa tgaagaatta aaaaataatt
     1501 ttaataactc atttttagat aaaaaaacaa ataaagatat agtgttatta gtagaaaatg
     1561 aactgagaga tattgtgaat gtagaatata ttaatcaaga aagtagttta gataatcaca
     1621 ataaaaaaca agttaattta gaaataaaag ttaatggaga acttgcaaat ttgttgaaac
     1681 ttgaaggtgt aatgaagaag ttaagactaa ataataaact tgaaaagata gaaattaata
     1741 aattaaaagt caataatttt gatgttcaaa atattccaaa tgaatataaa caagaaaaag
     1801 acaatattgt tgaatgtaaa atgacagtta gagtcatata acaaaaaaga atagataaag
     1861 taaaaaacta ttatcaatta ataaaatttt atagaggaat tagttttacg ataattcatt
     1921 tctacaagag ggattgttga tgattttatc gaaatcctaa ttaaagttaa tatattgtac
     1981 caaaatataa gtgaatttaa tagaatattt acttatacta tagattgatt aataaaaaaa
     2041 tggtataata acaaattgta ctttaattta ataataaggg agtggaagtc atattgttag
     2101 ggagaaatga gctatgccct tgtggaagtg gcaaaaaata caagaaatgt tgtttaaata
     2161 aagatattgt ttcagaaaga gcatgcagaa aaactgaact ttcacaaaaa caatatacag
     2221 atttatattc aaagttatat gattattcaa gacaggataa gtttaaagaa gaatgtgaaa
     2281 aagctaaaga aatgttttat ataatgcaaa atgatagtgt aaatgaaaaa tttgaaagat
     2341 tttttaatac ttatttaata caagaccaca taatggaaaa taaaaaggtt atgacagttg
     2401 gttttcttga agaaaatggt gcaaacctaa gtcaatcaga agtatcaata ttaaaaagta
     2461 tgtttgaatc atacgtaagt atttatgagg taaaagaagt ttctgctgga aaaatagtat
     2521 taaaggactg tttaagtgga aatgaacttt gtacagaaga tgtaaaactt ctgagaagtt
     2581 ttaaaatcgg aagttgtatg atagctagaa tagttgatat tgaaggaact aacatattga
     2641 tagatataac tataagtata tcaaatgagg ttaaagatat aattttaaag gatattatga
     2701 atctattcaa tcaatatcaa gatgtataca aagatatgaa gtcgttctta atttatcata
     2761 cacatatact atacaagtat atacaacagc ttttagaccc aagtatagct gaatatttaa
     2821 agagagaaag agataaaaac gatacaaaag ttaattcaga aaatgaatta gctgaagaag
     2881 aatgtaaagt tttagatact cttaaagaaa atgtagaaaa agaaaatata gatgcatgta
     2941 tagagttttg gaatgaatat aagaaaaatc atgaaaatat aaaaggagct gaaaatggtt
     3001 gggcagctgc tgttgaatac tatgttaaaa aagaagcagg acaacctata actcaagttc
     3061 aaatatcaaa gaaatacgat ataagctcta gtacactagg aaagagatac aaggatttaa
     3121 aaatatctta attaattata aaaagcttta agtcatagat aaagaatcta tagtataata
     3181 tatgtataat gtttagaaag aggtattaat aatgagtctt agtttaggaa atgcaattgc
     3241 tgtatcaacg aactttataa aatctaaccc agctgtacct gtgtacaatg gagaagaaat
     3301 atatgcagtt atttatcata tagaaatagg atataataat ggtgtatcac ctgataccaa
     3361 acaatatata ataatagact ataaagagtt tgatttatct atggataaaa aaaatgaact
     3421 aataaatatt gctaaagagt actgtaaatc taaaggtatt gtagaaaacg aaaaccttga
     3481 aataaacctt ttagacaatg aagaagcaga gaacttctta gaaaaggtgt ttaataatat
     3541 gaaggttata agaggtctta taactaaaaa aggataaata aatggactat ataggaatag
     3601 aaaatataac accttatgaa aatacatatg aatttagtgt atatgaatat gatgatgaaa
     3661 tcaccttagg tagtgaaaag ttatatgtat gtgaattaag ggttgtattg attaaagtta
     3721 attctctgta tgttgaaaga ttgcataaat cagttgaagc aatggtctta gtaaaaaact
     3781 tgaaaaaaga tttagataaa acacttgttg taaacaaaat aaagaatttt gtgctagatg
     3841 agatttgggt agaaaatcta gtaaaagaga atatagaagt tatatttgta gaaagctagg
     3901 aataacccta gcttttttat attacaatct aagagaggaa aacaatatgt taagagtatc
     3961 taatgtaaaa cttggaattg ataaagatat atctttatta aaagatgtta tacttaagaa
     4021 gctaaaaata aaagaaaaaa acttagtcaa atatagtata tataaagaat ctatagatgc
     4081 tagaaaaaaa ggaaaaatgg aatttgttta ttctgtagat gtagaagtta aagatgaaaa
     4141 taaaatactt aactcaaaaa taaaagatgt aacaaaaata aaggaaatca gatacgtaaa
     4201 tgtacctgtg ggtgataaaa aattaaagaa taatcctttg gtaattggta gtggaccagc
     4261 tggtttgttt gcaggtctgc ttttagctca aatgggttat gaaccaatca tacttgaaag
     4321 aggtcttgat gtagacagta gaaccaaaga tataagtgat ttttggacaa ctagaaaatt
     4381 aaaaaataat tcaaacgttc aatttggaga aggtggagca ggaacttttt cggatggtaa
     4441 acttacaact agaataaaag atatacgatg tagaaaagta ttagaagagt tagttcagtt
     4501 tggggcacca gatgaaatat tatactcaca taagccacat gttggaacag atatattaaa
     4561 aaatgtagtt aaaaatataa gaaatgaaat aataagttta ggtggtcaag ttagatttaa
     4621 ttcaaaagta acagatatag ttgtatcaaa tgattctata caatctgtaa taataaatga
     4681 tactgaaaaa atagatacag atacaattat attagctgtt ggacatagtg caagagatac
     4741 ttatgaaatg ttgtattcaa gaggtataaa aatagttcaa aaaccttttg caataggagc
     4801 tagagttgaa catccacaag atttaataaa taaagcacag tacaaggatt tttataatca
     4861 ccctaggtta ggagctgcag attatagact tatagagcat gtttcaaatg gaagaacagc
     4921 atatactttt tgtatgtgtc caggaggtag tgtcatagca tctgcatcaa atgattttga
     4981 aatagttaca aatggtatga gtgaacatgc aagagataaa ataaatgcaa atagtgcatt
     5041 tttagtaaat gtaacgccag aagattttaa gagtgataat ccactagcag gtgttcattt
     5101 ccagcagaag tatgaaaaat tagcttttga attaggtgga aaaaattata atgcaccagt
     5161 tcagcttgtt ggagatttta taaacaataa gacttctttg aatttaggaa gtgttgagcc
     5221 atcatataga ccaggatata cacttacaaa tttaagtgaa tgtttgccta gcttcgtaac
     5281 agagactatg agagaagcgt ttatatctct tgataaaaaa ttgaatgggt ttgcaatgta
     5341 tgatgctgtt ttgacgggag tagaaactag gtcatcatca ccaataagaa tagtgagaga
     5401 tgaaaatact ttagaatcag tgagtattaa gaatctatat ccatgtggtg aaggtgctgg
     5461 atatgcaggt ggtatagtta cagcagcagt tgatggtata aaatgtgcag aaaaaataat
     5521 acaaaaatac accaataata caaaaattta acataaactt aatgattttt taatatttaa
     5581 catgaattta acattatcac tcctataatt tgataaaata caataggtta taaaattttt
     5641 agatacagta atctctttac aaggaggaaa taatttgaac attataataa gtctaatggg
     5701 tggattaggt ctgttcttat atggtatgaa ccttatggga gaaggtcttc aaaaatctgc
     5761 tgggacaaag ctaaaaaaga taataaaact acttactagt aacctattta tgggagtttt
     5821 ggtaggtaca ggagttacag cagtaataca aagttcaagt gctactactg taatggttgt
     5881 tggtttcgta aatgcaggaa taatgacttt aaaacaggca ataggagtta taatgggtgc
     5941 taatataggg actactgtta cagcccaatt ggtatctttc gacttaactg gtatggcccc
     6001 agtagcatta ggtgtaggta ttatacttta tttatttgga aataagccta gaataaaaaa
     6061 tatagccgaa atacttatag gttttggtat attatttaca ggtatggatt ttatgaagat
     6121 ggcagtggag ccactaagag actatcaagg atttactgac ttacttgtta catttggtag
     6181 atatccacta cttggattat tattgggatt tgggattaca gcaataatac aaagttcaag
     6241 tgcatctatg ggtatgttgg ttgcactggc agcagaaggt ctagtgccac tatcagcagc
     6301 actgccaatt ctttatggcc aaaatattgg tacatgtgta acttcactgt tatcaagtat
     6361 tggtgctaac aaaaatgcac gaagagcagc tatgatgcat ctaatcttca atgttttagg
     6421 aacggtgata ttcttaatat tcttgaataa accagtagta agcatggtta caagttggga
     6481 tccaagcaat gttgcaagac aaatagccaa tacacataca ttgtttaata taatatctgt
     6541 attgatactt ttaccattta caagtcttat tataaaacta gctataaagc ttgttcctga
     6601 tagagcaggt gatatagatg aagatgaaac taaaactatt aaatatatag atgatagaat
     6661 gatagaaact ccttctatag ctcttgcaaa tacgataaaa gaagcacttc gtatgggtga
     6721 gaaggcgaag gaaagcctaa atgcatctat ggaagcttta gtagaacatt caactgaaaa
     6781 aatagataag acatatagac gagagagatt aatcaatgat ttacagaaag ctattttaaa
     6841 ttatttactt aaactttcaa aagcagcttt agatgatgat tcaagagaag ttgtggatac
     6901 tttgtttaat acagtcaatg atatagaaag aattggtgac cacgctaaaa atatagctga
     6961 attatcacaa gttgcaatag attcaaatat ttatttctca gaagaaggac aatctgaact
     7021 tgatgttatg tataatagag ttgtttcagc atatacttat gcattagaat caatgaggag
     7081 cgataatgtt gatttagctt gcaaggttat aaaaattgaa gaacaagttg atataatgga
     7141 gaaatcttgt agagctaatc acatgtatag attgaataat aatctatgta gtatagaaaa
     7201 tggagttatt tatttagatg taataagtaa tttagagaga atatctgacc atgctgtcaa
     7261 tatagcacaa caagttatag cgaagagatt aggaaatgat taaaaataat taatttaaaa
     7321 taatataaaa tttaaaatag aggataaata aaagagaaac tttaaatgat acgtttagag
     7381 tttctttttt tgtaaattta tttgactaaa atttttaaat ttatacatag ttatagtatt
     7441 tagaagaatc gatttcctga taataaatga tgagatatgt atgattttgt ggcaaacttt
     7501 taaaaaaaac acctatattt tgtgttataa tctctttaaa acgaatatgc tgaaaagagg
     7561 gatttaaatg ggaaaaataa attttgatta tagtaaagct acagagtttt tttgtcaaaa
     7621 cgaaatagat gtcatgcaac catatgtaga tgttgcgcac gacatgttac ataataaaac
     7681 tggtttagga aatacttttt taggatggat agaccttcct aaaaattatg ataaagaaga
     7741 gtttgacaga ataaaaaaat ctgctgataa gataaaatca gactcagatg ttcttttggt
     7801 aataggtata ggaggctctt atttaggttc gagagctgct atagatatgg taagtcattc
     7861 ttttaggaat ggacttaaaa aagagcaaag aaaggctcca gaagtatatt ttgtagggca
     7921 taatataagc tcaacatata ttatggattt attagatata attgaaggaa aggatatttc
     7981 tgtaaatgta atatctaaat caggaactac tacagaacca gctctggctt ttagaatatt
     8041 taaagattac ttagagaaaa agtatggtaa ggaagaagca agaaagagaa tttatgcaac
     8101 aacagatgca agtaaaggtg cacttagaca gttagctaca gaagaaggat atgagacttt
     8161 tgtaatacca gatgatgtag gtggtcgttt ttctgtattg acagcagttg gattacttcc
     8221 tattgctgct gctggacttg atattgatgc tatgatgaaa ggcgcaaatg atgctagaga
     8281 agcatttcaa aatcctgact taaaaagtaa tgatagttat agatatgctg ttgctagaac
     8341 aattttacat agaaaaggaa aagatgtaga gttattagtg aactatgaac ctcaactaca
     8401 ttatgtaagt gagtggtgga aacagcttta tggagaaagt gaaggaaaag aaaataaagg
     8461 tttattccct gcttctgtag atttctcaac agatttacat tctatgggtc aatacataca
     8521 agatggaaaa aggcttttat ttgagacagt acttaatgtt gaaaactgta agagaaatat
     8581 aactataagt tcagaggaag tagacttaga tggtttaaat tatttagcag gtaagactgt
     8641 agattttgta aatcataaag cttttgaagg aactctattg gctcatacag atggaaaagt
     8701 acctaattta gtaataaata tacctcaact agatgagtat aattttggat atttagttta
     8761 tttctttgag aaggcttgtg gaataagtgg atatttattg ggagttaatc catttgacca
     8821 accaggagta gaagcttaca agaaaaatat gtttgcttta ctaggcaaac caggttatga
     8881 aaaagaaaag gaagagctag aaaaaagact taaataattt taaatagtta aggtttaatt
     8941 aagattaaaa taatataatg aaatcatcaa aaaggagttt actcaaaata gatgatttca
     9001 ttttttttat gaaaaattgg gaaaaatata ttttgataat ttatattata ttaagatagg
     9061 gagtggtatg agatgtcaag aagaaagaaa gggataagtt tagtaatatt agttgctata
     9121 ataagttcaa tacttagtag ttttctaaca ataattttag ttaaggacaa tttagtaagc
     9181 aaatctacag gaagttctac gccgatagtt gtaaatgatg atggtaagag tcaaaatatt
     9241 tatcaagcag ttgcagaaaa agctacgcca tctgttgtag gtattacaac aactagtgtt
     9301 gatactagta atatgtttgc aataccaact gaaacacaag gtgtaggaac tggaatcata
     9361 gttgattcaa atggatatat acttactaat tctcatgtta tatcagatgg tcaagctaca
     9421 agtgtaaatg tattattcaa tgatggttca acaacttcag gtaaagtagt atggtttgac
     9481 caacaacttg atttagctat agttaaggtt gataaaactg gactaacacc agcagaattt
     9541 gctgatagtg acaaagtaaa agttggagat atatcaatag caataggtaa tcctcttgga
     9601 ttagattttc aaaaaacagt tactcaaggg ataataagtg gattagatag aactatacaa
     9661 acagaaaaaa ctaatatgac tggtttatta caaactgatg caagtataaa tgcaggaaat
     9721 agtggaggac cattgttaaa tcaaaaaggt caagtaattg gtataaacac agcaaaagct
     9781 tctcaagcag aaggattagg atttgcaata ccaatcaata cagctaagag tatagttgaa
     9841 gaagtaatca aaaatggaaa atatgaaaaa gtaactttag gcataaaagg tacagatgta
     9901 tcaaattatg aagcagcaac aggtacaaaa ttaagtactg ataaaggtgt ttatgttgca
     9961 gaagtaatat ctggttcatc agcagaaaaa gctggagtaa aggtagggga tattataact
    10021 aaagtaggag atacagatat aacaggtatg aatgatttaa ataaaaagtt gtatactttc
    10081 tcaaaaggtg ctagtacaaa aataacagta aatagaggcg gaaaagcagt aacaataaat
    10141 gtgaatttct aaatatgacc aaaaagatag agactacatg gtaaagtgta gtctctattt
    10201 ttatttaatt tttatagtat atacatctaa attaattaaa atgatgtatt gtttatagtt
    10261 tattgctata ttttcttata aaaataaatt taataaatat ttaaatttaa tttacaaaaa
    10321 caatataaat atcataataa aataaaattt tttaatatta ctaaaaatat atagacaaaa
    10381 taataacaaa tatagtatta tgtaattgta gaaatgcttt tcataggtat attctatgaa
    10441 gatgaattgc ataaaaatga aaaaatggaa tggatattat ttaggggtga aaaatcatga
    10501 atccattagt tataaactta caaaaatgta gcatacatga tggaccaggg ataagaagta
    10561 cagtattttt taaagggtgt ccacttgaat gtgtttggtg tcataatcca gagagtcaaa
    10621 catatacaaa acaagttttg tataatgaag aaagatgttc aaaatgtgaa gcatgtataa
    10681 atatatgccc tcataaggca atttacaaag gtgaaacaaa aatatgctta gaccaagata
    10741 agtgtgagtt ttgtgaaact tgcttagact attgtgtaaa caatgcaaga gagattgttg
    10801 gtcaagagta ttctgtaaga gatttggtta aagagataga gaaggataga attttctatg
    10861 aagaatcagg tggtggagta actctatcag gtggagaagt aatggctcaa gatatggact
    10921 tcatttgtgg agtaataaac atgtgcaaat caaagggaat ccatgtagca attgatacat
    10981 gtggctatgc aaagtcagaa aattatgaaa gagttgctaa atgtgcagat ttgtttttat
    11041 atgatattaa gcttattgat gaagataagc acataaaatt tacaggaaaa tcaaatgact
    11101 taatactgaa aaatgttaag attttaagtg aattaggggc taacataaat ataagaatac
    11161 cattaattgt aggagtaaat gtagatgatg aaaatttaga ggttaaaaaa atgatagagt
    11221 ttttaaagcc tttgaatata caagcagtaa gtcttcttcc atatcataat attggtaaac
    11281 acaagtatga taaaatttat aaaaagtatg aaggagaaga actacaaaga ccatcagaag
    11341 aaaaattaga ggaaataaaa agactgtttg aagcaagtaa tttcaatact aagattggag
    11401 ggtaataaaa tggcaagagg aacttttgag agaactaaaa aattaagaga agaaagtatt
    11461 aatgcagaac cacacatatc tattgaaaga gcagtactta tgacagaagc ttataaaaaa
    11521 tatgagggaa gtgtagagat tcctgtactt cgtgctttat catttaagca ttatattgag
    11581 aatagaactc ttagcataaa tgatggagaa ttaatagtag gtgaaaaagg tgattctcct
    11641 aatggagcac caacatatcc agaaatttgt tgtcatacaa tggaagattt agaagtaatg
    11701 cacaatagag atataataaa ttttagcgta tcagaagaag ctagaaaaat acataaagaa
    11761 gaaataattc ctttttggaa aaagagacaa acaagagata agataataaa tgcaatgaca
    11821 cctgagtggt tagcagcata tgaagcaggt atgtttacag agtttatgga acaaagagcc
    11881 ccaggacata cagtttgtgg agatacaata tataaaaaag gatttttaga tttaaagaaa
    11941 gacatagaag caagattaaa agagctagat tttcttaatg atttagatgc atataataaa
    12001 aaagcagatt tagaagctat ggcaatagct tgtgatgcaa tggttatatt aggtaaaaga
    12061 tatgctgaga aagcaagaca aatggcagaa gaagaaacag atgaagctaa gaaacaagat
    12121 ttattactaa ttgctgaaac ttgtgatgtt gtaccagctc acaaaccaga aacttatcat
    12181 caagctatac aaatgtattg gtttgttcat ataggggtta ctactgaact taatatatgg
    12241 gatgctttta ctccaggaag acttgaccaa catctaaatc ctttctatga aagagatgta
    12301 gaaaatggaa tactagatag agatagagca caagaattac ttgaatgttt atgggttaaa
    12361 tttaacaatc agccagcacc accaaaagtt ggtataacat taaaagaaag tagcacatat
    12421 acagattttg caaatataaa cactggtgga ataaatccag atggtcaaga tggtgttaat
    12481 gaagttagtt atataatact tgatgttatg gatgaaatga aattgataca acctagttca
    12541 aatgttcaaa taagtaaaaa gactcctcaa aaattcttaa agagagcttg tgaaatatca
    12601 agaaaaggat ggggtcaacc agcattttat aatactgaag caatagttca agagcttatg
    12661 gaagctggaa aaactataga agatgcaaga cttggtggaa ccagtggttg tgttgaaact
    12721 ggatgttttg gtaaagaagc atatgttcta actggatata tgaacattcc aaaaatactt
    12781 gaattaacat taaataatgg atatgaccca attagtaaaa aacaaatagg tattgaaact
    12841 ggagacccta gaaatttcca atcttatgag gaactatttg aagcatttaa aaagcaatta
    12901 cattatatga ttgacataaa aatagaagga aatgctgtga tagaaaatat atgtgcaaaa
    12961 cacatgcctt gtccacttat gtctacaata gttgatgatt gtatagaaaa aggcaaggat
    13021 taccaaagag gtggagcaag atacaacact agatatatac aaggtgttgg tataggaact
    13081 ataacagata gtttaacagc aataaaatat aatgtatttg ataagaaaaa attcgatatg
    13141 gatacattgc ttaaagcact tgatgctaat tttgaaggat atgaagctat acttaattta
    13201 gtttcaaata agactcctaa atatggaaat gatgatgatt atgctgatga aataatgcaa
    13261 gagatattca atgcatatta caatgaggta actggtagac caactgtttg tggtggagaa
    13321 tacagagtag atatgttacc aacaacttgt catatatact ttggagaaat tatgggagca
    13381 agtccaaatg gtagactttg tgcaaaacca gtttcagagg gaatttcacc tgaaaaaggt
    13441 ggagatacta atggacctac agcagttata aaatcttgtg ctaagatgga ccacataaaa
    13501 actggtggaa cattattaaa tcaaagattt gcaccatcag tagttcaagg agaaaaaggg
    13561 ttagataata tggctaatct agtaagagca tactttaata tggatggtca tcatatacaa
    13621 tttaatgtat ttgataaaaa tgtactgtta gaggctcaaa agaatcctca ggattataaa
    13681 gacttaatag ttagagttgc aggatatagt gaccatttca ataatttaag tagaacacta
    13741 caagatgaga taataggaag aactgagcaa acattctagg tttgcttgta aataaaactt
    13801 cttactatat aaaatcgtaa ctgatataat aaagtataaa tggtttgatt ccatttttaa
    13861 aattttagat taattatgag ctaactttct aaataggaag ttagctcgtt aagtttaatg
    13921 aaaaaatcgt taaatttata tttagtaaat catacacata ttagtaagta atacatacaa
    13981 aagaataatt tattatagtt ataataaggt tatattttat aaaaataaga taattttaag
    14041 cagttaaaat aaagtcaatt tagctacttt aaaataaaac taatagaaaa aaggagattt
    14101 gatatgaaaa ctttaggatt tattggttca gggaatatgg gaagtgcaat gataggtggc
    14161 atagtacagt caggattagt aaaggcagaa gatataactg tatcagattt aagccaagtt
    14221 gccttagata aagtaaaaga agaatttgga gtaaatacta caactgattc aaaggtagct
    14281 gttacaaatt ctgatgtggt aatagtagca cttaaaccta atatatgcga aaaggcttta
    14341 actccactta aagaattaat agatgcaaat aaaataatag tatctatagc agcaggtaaa
    14401 actatagaaa gtctggaaag ttttataggt tcagataaaa aaatagtaag agtaatgcct
    14461 aatactccag cccttgttgg cgaaggaatg tcagctcttt gtaaaaatag taatgtaaca
    14521 gatgaagagt taaatatgat taaggctctg tttgaatctt ttggtgaggc agaaatagta
    14581 tcagaatatt taatggatac agttacaggt attagtggtt cttctcctgc atatgtattt
    14641 atgtttatag aagctatggc agatgcagca gtattagctg gaatgccaag acaacaagct
    14701 tataaatttg catctcaagc agtaatgggg tcagctaaga tggttcttga aactggaaaa
    14761 catccaggtg aattaaaaga tatggtttgt tctccagcag gaactactat agaagctgtt
    14821 aaggtattag aagaagaagg ctttagagca gcagttataa aatctattgt tgcttgtata
    14881 gataagtcta aagatatgag taaataacag atgtaattaa gtttactata tggtttcttg
    14941 aattgttttt ttagtgctta tttatatcta tacataaatg tatttggtat aagtaaaata
    15001 agagtaattt actataaaaa ggaattta
//
LOCUS       DBGJFU010000092        14727 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.092, whole genome shotgun sequence.
ACCESSION   DBGJFU010000092 DBGJFU010000000
VERSION     DBGJFU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.092"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            111..1178
                     /locus_tag="ACOIAS_002665"
     CDS             111..1178
                     /locus_tag="ACOIAS_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HGZ2702367.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1197..2357
                     /locus_tag="ACOIAS_002666"
     CDS             1197..2357
                     /locus_tag="ACOIAS_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HGZ2702368.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2370..4466
                     /locus_tag="ACOIAS_002667"
     CDS             2370..4466
                     /locus_tag="ACOIAS_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HGZ2702369.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(4625..4969)
                     /locus_tag="ACOIAS_002668"
     CDS             complement(4625..4969)
                     /locus_tag="ACOIAS_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGZ2702370.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            5261..6151
                     /locus_tag="ACOIAS_002669"
     CDS             5261..6151
                     /locus_tag="ACOIAS_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2702371.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            6369..7655
                     /locus_tag="ACOIAS_002670"
     CDS             6369..7655
                     /locus_tag="ACOIAS_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGZ2702372.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(7744..8187)
                     /locus_tag="ACOIAS_002671"
     CDS             complement(7744..8187)
                     /locus_tag="ACOIAS_002671"
                     /inference="COORDINATES: protein motif:HMM:NF025547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HGZ2702373.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(8211..9005)
                     /locus_tag="ACOIAS_002672"
     CDS             complement(8211..9005)
                     /locus_tag="ACOIAS_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HGZ2702374.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPYKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(9571..10047)
                     /locus_tag="ACOIAS_002673"
     CDS             complement(9571..10047)
                     /locus_tag="ACOIAS_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HGZ2702375.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(10148..11317)
                     /locus_tag="ACOIAS_002674"
     CDS             complement(10148..11317)
                     /locus_tag="ACOIAS_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2702376.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            11480..12343
                     /locus_tag="ACOIAS_002675"
     CDS             11480..12343
                     /locus_tag="ACOIAS_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HGZ2702377.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            12414..13007
                     /locus_tag="ACOIAS_002676"
     CDS             12414..13007
                     /locus_tag="ACOIAS_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HGZ2702378.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(13258..13893)
                     /locus_tag="ACOIAS_002677"
     CDS             complement(13258..13893)
                     /locus_tag="ACOIAS_002677"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HGZ2702379.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(13973..14704)
                     /locus_tag="ACOIAS_002678"
     CDS             complement(13973..14704)
                     /locus_tag="ACOIAS_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGZ2702380.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEGDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         5581 a         1742 c         2380 g         5024 t
ORIGIN      
        1 agagtaaatt ttcagtaaat tatcttgaca tatatattga agtaataata taatttaaag
       61 tataaaattt taaaaattga attataactt caaaaaataa ggagggtaat atgaagataa
      121 cagatatcaa atttgaaaag cttagaatta aattaaaaaa acctgtagta gtttcatttg
      181 gagtaattga gtatggagaa agtataattt taaaaattga aactgatgaa gggtattgtg
      241 gatttggcga agcagcacct cttgcagcag ttacaggtga agttttggat aatgttcttt
      301 cagtattgct tatgtttaaa aaagagttaa taggcaaaga cccactagat attgaaacca
      361 tacataccat catggatgga attattatag gtaatacttc tgcaaaagca gctattgaca
      421 tagctttata tgatattaaa ggaaaaatta tgaatgcacc tctttataaa gttcttggag
      481 gatttgatag taaagtccaa acggatatta ctataagtat agataagcca gaaaaaatgg
      541 cagtagaagc tttagaaaga gtaaaagaag gttttaggat attgaagctt aaagcaggta
      601 taaatcctga agacgatatt gaagctgtaa agttaattag agaagccgtt ggagagagta
      661 taagaattag aattgatgca aatcaaggat ggaacgtaaa tagcagtata aatactatca
      721 aaaagttaga agaatttaat gttgatgcaa ttgaacaagc acttccacat tgggatttag
      781 atggtactgc ttacataaga aataaaagta acacaaaaat tatgatagat gaatcattac
      841 attcaccaat agatgcaatt aaggctataa aaaagaatgc agttgataca tttaatatta
      901 agctgatgaa atcaagtgga atatatcctg ctataaaaat caacaacata gctgaggcaa
      961 gtggagtaaa ttgtatgtta ggatgtatgc tggaaacgag aattggtata acagcagcag
     1021 caaatttaat agcatctaaa aagaacataa cagaagctga ccttgatagt tttatgtttt
     1081 gtgaagaatt agaaggtata tctggaggat ttgtaatgga tggagatatt atgaatcttg
     1141 tcaataaacc aggtttagga atagaagtaa acttatgata aatttggagg ttaaatatga
     1201 atacactttt ttcaaatcca ggtagtatac ttgctgtaat gacatcaatg attgcattgg
     1261 gtttttactt acaaagatac aaagccatta aaagcttagg accagcactt acaataatca
     1321 taatgggaat aatactttca aacttaaaag tagttccagt aagtactgag ttatatggaa
     1381 ctatttcaac atatgctatt ccaatttcta tgactataat gttgatgagt gttgacttaa
     1441 aagaaatgac aaaattatca agagaacctt taattgcaat ttttgtagct gtattaactg
     1501 taagtatcat ggcattttta tttggattag tatttgcaga aaaaatatca gaaggttgga
     1561 aggttgctgg aatgtttgta ggtacatata ctggtggaag tgctaatctt acagctattg
     1621 gaacaggtct taatgtaagt agacaaactc ttgcagcagc aaatgcagca gactatgtaa
     1681 ttggagtacc aacattgata tttatgtttg ctctaccagc aatattaaaa aattcaaaga
     1741 agtttaaaaa actttggcca tatcatgtag aagaatcaga attagaagat tgtcaaaatg
     1801 aagaatttat ggagtcaaaa gaatggagta ttaaagatat agcttggatg ttggctatag
     1861 gttttgttgt tacagaagtt tcaacaatac ttgctggata tttcaattca agttttagta
     1921 gtgcagcaag aatactttta gttacaacaa tttcaattat aattgctcaa ttaaagccag
     1981 taaagaaact aaagggaaat ttagatttag gattatttgt tgcattattc tttttatgta
     2041 caataggttt ttcagtagat ataaaagagt ttttaggttc aacttttact ataacacttt
     2101 actgttttag tataattttt gcttcatttg ttttccattt aggcataact agacttttaa
     2161 agataaagta tcaatatgta atactttcta tagtaggagc aattgctgat ggtcctactt
     2221 cagctttagt agctgcttct gcaaagtgga attcgcttgt aagtgtagca gtcgtaatgg
     2281 gagtaattgg tggagtatta ggaaattatg caggtatatc agtggcatat gcaataaaga
     2341 tgtctctagg attatagggg ggagtttaat tgataaaatt taaattatac atatctggac
     2401 tatacagtgg aaatgtaatc tttgatggag atttattaat tgaaaagttg aatcctttta
     2461 caaataaaat agaaagttta aaaccaataa gcaaagaaga aaatacatat tatttaaatc
     2521 ttacaaaaat agatttaaag tctttattta ataattttga tgtatatact aaaagcttag
     2581 taaacacaga taaagacaca gttataaata acttaggaga aacgcattct aaacttaatg
     2641 aatatatatg ggttcaaaga aacaaaaagt tcccactaga tataattatt gtagataata
     2701 aaatagtagg ttttatctgt ttatctagag aaacttgtac tatattaatt atggatggat
     2761 atgaagagta tactgtatta aaagaatggg aaaaaactca taaaaatgaa gagatatatt
     2821 ctataagatt tggtggaaat tatatgattg atatgaaaga tggtataaaa ctttctacag
     2881 atgtatatct ccctgatttt gtagattctg ccaaaaaagc accgacaatt cttatgagaa
     2941 ctccatatgg aaaagaaaat gataaagaaa tatattataa atatgtacaa agaggatatg
     3001 ctgttgtaat acaagatgtt aggggaagaa atgaatcaga aggtaagtgg gaacctatga
     3061 ttcatgagag ggaagatgga gatagtacca taaattggat agtatcacag gaatggtcaa
     3121 gtggaatagt tggaatgctt ggagcatcat atcttggtta tgtccaatgg gcagctgcat
     3181 cttcaggcaa taagcatctt aaagctttag ttagtatagt aacatcagga agccctttta
     3241 ttgatatacc aagaaaagga ggagcttttg tatctggtat gcttgcatgg gcatttatgg
     3301 tatcaagaaa taaagttgat agaagcaaaa tggtaagaga tgattgggat gatgttttaa
     3361 acatacgacc aatagaaaat atacctgttg aagctcttgg atatagaatt gaatttctgg
     3421 aagaatggtt aaagagagta gaaaaagatg agtattggga tttgatggac tggcatttac
     3481 aaaaagataa aataaatgta cctgcattgg ttgtatctgg gtggtatgat gataatagta
     3541 tgggaactac agaggcctta gatgttataa aggactatga aaaaggaaag agaaaggcta
     3601 ttctaggtcc atggatgcat aattcaaata ctttaagaga tataaatggt atttctttag
     3661 gaaatagttc attaagatat gatttggatt ataattatct attatggttt gataaatact
     3721 taaaaggtat agaaaataac atagatacga ctgctccagt agagtattac tctgtaggtt
     3781 ctaataagtg gaagacagaa gaaaattggc caataataaa taagatagat aaaagtatgt
     3841 atctaataag tgatggaaat gctaacacat ctttaggaaa tggtagatta gtatttgata
     3901 atgatttaga agaaaagtat gatagttata tatataatcc aaaagaccca tcagtacaac
     3961 taattgatat gtcagagaat gaagttggag ttccaaataa ctacaaagat ttagaaaaaa
     4021 gaagtgatat gttgtgttat acatcagatg cttttagtga agaatttact gtaacaggtg
     4081 atataaagtt agagtttttt gcatcaagtt ctgccaaaga tactgactgg gttataaaaa
     4141 ttatggacgt ggatttagat ggaaattcaa ttaaattagc tgatggtata cttagtgcta
     4201 gatttagaaa tagtttttat aagtcagagt ttatggaaga aggagagatt tataagttta
     4261 cgattataac ttcaaaaata tcaaacacgt ttaaagttgg tcataaaatt agactagata
     4321 taacgtctag tgctaaaaac tttattttcc aaaatagcaa cacgacagag ggatataata
     4381 gtattgaata tatagaagca aagaatacga tttaccatgg aggtaaatat ccatctaaat
     4441 tgatattgcc aatagagaat aagtaaattt atatagatta aatagtgagg aggtgtctca
     4501 aaatgaattt tttagttcgt gagacacttt cttttatacg aaatcaaata tattttcatc
     4561 atttcaacaa tgaaaaagag gcttatctct ctgttttgag atagcctcct ataagctaaa
     4621 ttgattattt tgtagctgca acaacgctca tttcaactaa caaatgctct ctagcaagtc
     4681 tagcttcaac acaagctcta gcaggttcaa atcctttttc aacccaagca tcccaaacag
     4741 agttcatagc ttcaaagtct ttcatatctc ttaaataaat agtaacagat agcaaatgtt
     4801 gcttatctga accatatttg tttaataaat cttctatttt agctaaaact tcagttgttt
     4861 gttcaattac tcctccttca gcatgagttt gtccacataa ataaactgta ttgttatgaa
     4921 ctacagctct actcattctt ccagttcctt catatctctt tatttccata ttttaaatac
     4981 ctccatagtt aaaaaatcta ttaaaaatct aaataataaa ttaaaaaaag tctatactat
     5041 ataaaattat attatatatt ctgataataa tccataaaaa catataactt tttagctata
     5101 attattattg ttattaattg aaaagattta ttagttactt gacatagttt acacttttaa
     5161 ttatagtata aattggaata tttagataat tattatttaa tgtataagct aagtttttaa
     5221 ttaatatggc tttatacatt tatatagaaa agaggtacgt atgagtagca caatcaaatc
     5281 aaatcgaata aaaggtataa tgtttataat agcatcagcg cttggctttg caatgatgtc
     5341 tgcattcgta aagctagctg gagatttacc aagttttcaa aaagtgttct ttagaaatct
     5401 ggtatcagct ataatagctt tttggcttat cataaaacat aaaggaagtc taactggtaa
     5461 aaaagaaaat ttaaaagtac ttttatatag gtcaatattt ggaactctag gagttatatt
     5521 taactactat gcaattgaca agttgatgct ttcagatgct aatatgctaa ataaaatgag
     5581 tcccttcctt gtagtaatat tttgtgcaat tgctctaaaa gaaaaaataa atgtaaagca
     5641 gataggagct attataattg catttatagg agctttattc ataataaaac caacatttag
     5701 tgtagaagta attccatatc ttggaggaat tgcaggagct gtatttgcag caatggcata
     5761 tacttgttta agagttcttg gagatagaga agattactat acaattgtat ttttcttttc
     5821 agttttttca ttagttactg tagggccaat tgcttttgca gtatatgaac caatgaccct
     5881 tatgcaactt atatatctat tactaggtgg agtatttgca agtctaggtc aatttggaat
     5941 aactctagca tataaatatg cacctgctaa ggaaatctct atatttgact atagtaatat
     6001 aatcttttca gcaattttaa gtatattctt attcaatgtc tatccagata tgttaagtgt
     6061 tgttggatat ttaatagtat tttcggcagc attttatatg tttttataca ataagaagtt
     6121 agataaatta gacaaagaaa agaagaagta aactagataa aagtgttttt ataatttgta
     6181 tttaatattt aagtctttgt attaaaatta aagaaaattt aattaaaaat gtttggtata
     6241 ttaacttata acagcagtta aaattgaaaa attataactg ctgtttcttt tattaaaaat
     6301 atccaaatgt ctgtaaaaat agactataat agagtagtat aaataatata aaggggataa
     6361 attaaagtat ggaaaaagtg aaagttgctg tgattggagc aggtaataga ggaacgtatg
     6421 catatgctcc atatatttat gaaaattctg atgtatgtga aatagttgct gtagcagaac
     6481 ctaaaaaagg aagacgagaa ctatttacac aaaaatataa cctagatagt aaaaatgtat
     6541 ttgaaacttt agaagacttt tttaaacatg acaagatggc agatgcagta ataatagcta
     6601 caaatgatga tagacattat gatgtagcaa aactagctct tgaaaaaggt tatcatgtac
     6661 tattagaaaa gccaatgtct aatagtttag atggtctggt acatatagat gacttgtgtg
     6721 ataagtataa agataagatt tttatgattt gccatgtact gagatattct ccattttata
     6781 ataaattaaa agaaattgta gaaagcaaaa aattaggaga gctagttagt atacaatata
     6841 atgaaaatat aggatattgg cattttgcac atagctttac tagagggaat tggagaaata
     6901 gcaatgaaac cagcccttta attttagcaa agagttgtca tgacatggat attttgttat
     6961 atttagtagg aagtagatgc aaaaaaatat ctgcatttgg aagcttaaag catcttaata
     7021 accaaaatgc tagtggagag atggcacaaa attgtcttca gtgtttagtt gaaaaaaaat
     7081 gtccatattc agcaaagaga atttatttag aaaaagatag aagtataaat agagctgttc
     7141 acataaatcc aactgaagag aatctattga atatacttaa gacaagtcca tatggaagat
     7201 gtgtttacag atgtgataat aatgttgtag acaatatggt aaatatacta cagtttgaaa
     7261 atggagtgac ggctactttt aatttatgtg catttactaa agaaaatgga agaactataa
     7321 agttaatgtt tagccatggt gaagttggtg gggatttaaa taaaaatgag ataagaatta
     7381 aagaatttgg aaaaaacgaa gaaatagtta tgaatccatc aaatcaacag aatatggtag
     7441 aggaatatga tagaaatctt atagctgaat ttattaaatt agtttcaaat aaagaactag
     7501 aaaagggtag agtagcagca aaagaagcta tacaaagtca tgtaatggca tttgcagcag
     7561 aatattctag ggtcagtgat gaagtagtgt atatagaaga attttttgat agtgctaagc
     7621 aaatgactaa ggagatagaa gaaactatat tttaattaat aaaaatttat taaataaaat
     7681 aaagatagat taaataatag aaatacgaaa atatgtgttc tattaaggaa tctatctttt
     7741 ttattaaggt tttttcttat ccctatcaaa aaaatccata cattctgata aaagataaga
     7801 aagtaaagcg aataagattt ctgtacttaa aggaatcgtt actgaatcca taaaggtatc
     7861 aattatgttc atagctagaa aagttagtat aaaatcaatt ataaattcgc aaagtttata
     7921 tattgagtat ggtaatttgt ttacatatct gaatatatct aaaatagtag tgcatagaaa
     7981 atcaattgga gtggttatac aaaaatatat taaaaagaaa attaatacag ctccaagaga
     8041 tttatattca aatcctaaga atgataagaa gccaattcct ataaccgtga aaatacaaaa
     8101 agttatacca aaaaaaacag caaaaaataa aaatacagaa ataaattcgc ctaaaggaat
     8161 ccttctttta ttacttatct taatcaactc cttaatttat gatatgaaat ttatataaat
     8221 aataacattc ttaaaaattc ataatagtcc tctgtttcaa atagtactgt ttgagaatct
     8281 atttttttca tatttggata aaaagaggcc ttaaaagctt tataatgttc tctaaatttt
     8341 atttctatct taaatttata tggcaatttt attaagtgcc tagataaatc tccagataaa
     8401 gcttttttga cacctttttc aatttttttt agtgcaaggt ttggatggat tgaaattgaa
     8461 ccatttccaa ttccttttga cacaggaaca gtgactatat ttggatttag tttttttgca
     8521 ctctcacaaa gcatttcatc tccacttaaa aatgctacag gaacattata atatgcagca
     8581 atataagcat ttatagtaaa ttcagatgct atttcatcat ttattttaaa atagtcataa
     8641 tcaatatcca tagtatgaga aagtggatta ccattacaag tagctccaga gtgatagccg
     8701 gtgaatatac atgcatcaaa gctttcatca attcctgcca tcataactag tgggtctttg
     8761 gtccatcctc taagtatttt gacgttttca ggtaaaatat ttggattaat atttctacca
     8821 ctgtcatgtg catctttaac aagtatttca tgtacaccag attgagtagc tccatcacat
     8881 gctgctgtta tctctttact catttgcgtt ctatagtgtt gcgaaaattc acattcaagt
     8941 tttgtttcat cccaatttac aataccacaa gtaccttcta tatcagcact taaatacaat
     9001 ttcataatat ataccccctt atgtttatta ttgatgataa taacagataa cagaaaatac
     9061 ataatcaata aagggcataa aaatattatg tcttactatt aactattatt aaatctgtaa
     9121 ctgaaaaata actaaattgg tataaaaaaa tataacacta tgtgtcattt atttatatag
     9181 ttttttatat ttgatgttta tataaaattg taacatataa gtggttacat ttactagttt
     9241 aacatagtta aaatagttag gtattatttt taagttaaat tgattattat aaaatcgcag
     9301 aataaaagaa aaatttaatt tattaaaatg catgaaatgt tttttcctca acataatgct
     9361 aaattaaact caaaaagaaa taatagaaaa atcttatgta gcttagaaag aatgtgaatg
     9421 atttaaggga aaaatagaaa tttattatat aatattaggt tgtattatat aatacagcct
     9481 aatattatat gtatatgtga cataaaatct tggtaagtgt aaataaaata aatgtggaaa
     9541 acaagttaat gaaatcaaga aaaaagtaat ttaaaattca atttcatctt cccaattaaa
     9601 ttctcctgat ttttctacac tcttaggcca gtgactacac catttaggaa cggacggatt
     9661 ttcaacccta tcaacgcttg gagtatatgg ctttaagtca ataacaggac tattatcatt
     9721 agcatcaata tatggtattt ttattatgcc attttcatgg tcgatattga taatttgtac
     9781 agcagtcaat gctattggat tgggtcttat aggtgacctt gttgcaaata ttcctataac
     9841 gtctggtcca cttttataag gttttggggt ttctaaaata cttcttagtt caggattatc
     9901 aaacccatca gcccaccaaa atacatttaa atgactgaaa tcgtcgagtt catttaatgc
     9961 aggtacatac tttttgtcta atttaataaa gacctcttcg ccttgaatac atattttacc
    10021 tataggattt attaatattt ctttcataat attacctcca actatttaat ttatcatttg
    10081 taattttagg gtataacatg acatcatgtg agggtcaata taaaataata aataaaaata
    10141 ttaatagtta gaatgataat acatttttta catggagcat gacaccagta ataaggcaat
    10201 cttcgtcaat atcaaaagta gaggtatgca aaggggaagt aatgtttttt tcacgatttg
    10261 cacaaccaag gtggtaaaaa gctccttcac agtgtcttag aaaatacgaa aagtcttcag
    10321 caccaagagt gggcgagtct ttaaggataa tattttcttc accaaacaat tctacagcat
    10381 tttgtttgac gtaatctact agatggtttg aattgataag agctggataa ccttcctcaa
    10441 tttcaacaga aatacttcct ccaaatgttt tacaagtaaa atcgcaaatt tctctaattc
    10501 gtttttttga aaactctcta gtttcaggag tcaaggttct caaagtaccc cttataacca
    10561 ctttgtcaca aactatattt tctttgattc caccttcaat ctttcctaga gaaataacca
    10621 cagaatcagt tggatttgtg tttcgactaa cgattgtctg tagagctgta ataatatgag
    10681 cagatataac aatagcatca ataccttgat gtggataagc tccatgacat tttgaaccat
    10741 gtacagttaa ttgaagtgta ttggtggaag catttagagt gttgtatttt aattcaatta
    10801 gatttttgtt gatattggga tttacatgga gtccaaatat atattttaca tttggttttt
    10861 caagacatcc atcttctatc ataagttgag ccccaccaat agtttcctca gcaggttgaa
    10921 aaaagaattt tacatttaca tttaagtaat cctttaattt aaatagtata ttacaagtac
    10981 ccagtaaaat agcagtgtgt gcatcatgac cacatgcatg cattttgcca atattaatag
    11041 atttataaga ataattaagt tcttctgtta taggtaaggc atctatatca gctcttatag
    11101 caactgtttc aaagtttgga ctcacgttaa tgtatgcagt tatccctgta tgattcttat
    11161 attcaatata atttatacct atttcattta agtatttaat aatttttttc ttagtttgaa
    11221 attcttctaa tccaagttct ggagtcttat gtaaatctct tcttatattt ataacccaat
    11281 catttatctc atgacaaaga gtttttatat taagcataaa acacatccta actatattaa
    11341 aaatttattt ttcttcatta attatattat tttatgtaaa tcattattat gtatcaagta
    11401 caaataaaat atcttataat tatattagag atatttagta tggagaagaa tatatcattt
    11461 tataagaaga ggtgtttaaa tgttaggaat aattgcgcca tctgggccat taagaaatac
    11521 aagtttggag gaaataaagt ttaatttaga aagttatggt tacgaagtta agttttctga
    11581 aagttgtagt ttaaattata aaggttattt agctggtaat gatgatatta gagcaaggga
    11641 tatagaagat atgtttttag ataaagatgt agatataatt atgtgcttaa gaggaggtta
    11701 tggaacaaca agaatattgg ataaaataaa ttatgatata ataaagcaaa atccaaaacc
    11761 atttataggt ttttctgata taacaggttt aaatttagct ttctataaaa attgtggtct
    11821 tttaccatat catggaataa tggcagcaaa tgtaggtaaa tggggtaaat ttacctataa
    11881 atcattggta aatgctttag aatttaaaga tgaattatat ttagaaaatc caaaagaaga
    11941 aaagatttat actgtatgtg aaggtaaagc agagggaata ataatgggag gaaatttatc
    12001 cttaataatt gccacaatgg gaacaaaata tgaaatagat gcaaaagata aaattttgtt
    12061 tattgaggaa attggagaac ctcaatataa actggataga atgttaacac aactatattc
    12121 ttcaggaaaa cttgaggaat gtaatggtat aatttttgga gattttaagg attgtattga
    12181 agaaaatgat ttgatggaat tactaataga atttgcaaat aaggtcaata aaccttctat
    12241 atataatctt cagtcaggac attgtatccc aatgataact attcctttgg gcagaatgtg
    12301 cgaattagat gctacagaaa aaatcgttaa attaaaaaaa tagtgtgtat ataaatataa
    12361 tagaaatatg gtataatctt atacaagtaa aaatttagct aggaggatga aaaatgtctg
    12421 atgatagaat aatagacttt aacgaactaa agaacaaagt taaagattct gatgtagata
    12481 aatttgagca atacatatat aacttatatt tttctgttat ggatggaaag atgtctatgg
    12541 ctgagttttc aagaaaaata ttcgattata tgagagataa caacatatct caagaaaaat
    12601 ttatgaaaat acaaaagcaa tttatggaga gatatggtat ggacactgaa gaagtagaaa
    12661 aacaacttag aaactttgga atcgaccctt ctacagcagg ttttatgagt aataatactt
    12721 ccagcaaagt atctacagaa gatttagagt catttaagaa gagtgcagga ttttatgaga
    12781 aatatggaga aaaaatccaa ccaaagagtt gtataacaac atttataaaa aatgacttaa
    12841 atgatattaa tgtaataatc gaccaagaaa agataatgct ttgtagtgat agaaaaataa
    12901 atcttatgga ttcagaacta aatgagtttt tattagaata taagaatatg tttaataaaa
    12961 aaataaaggt agtaatgtgc gaaactacga ataagtatga ttattaaaaa atgaatttaa
    13021 atactgaagt agtataaatt aattgtacta cttaaaaata aaaatcgcaa tttattattc
    13081 cttataaatt tgtataagga atatctaaac tgcgattttt tatattgtga agattagtaa
    13141 taatagatag tgttttaaat tgtaatcttc tcactaaaac tgtgtttttc tattagaaat
    13201 tttattaaaa ataaatatct gataattgta ttatatggga tgtaaagtat tagaagctta
    13261 acgaatctct tttaatacat taactccatt ttgaatcata ctgttgaaaa atactaagtt
    13321 taaaatctca gcagggtgaa cgttaggaat atcataagta tctactaaat tcataggtac
    13381 aactatattt ttttcaatat tatgttgatt aaaatatgat tttagagtta ttgcaaattg
    13441 atatatacat atatctgtgc aatcgccaac tataattata ttatctatat tatcaaaatc
    13501 caaattttct aatgcaaaaa atccattagt agaattttta ggaagaattt gtaggttttc
    13561 tatcgactta agttcatcaa ccaattcact ttctacatca ttttccaaac agtgaacagg
    13621 ataactcaaa agttctattg atttaggagt atgagaatca gtaaatgcta ttactctatt
    13681 taatttgttt gatatttttt ttgtaaacat ttcaattggc tttattaaag actctactct
    13741 aggagagtat aatgcacctt gtcttgcaaa gccattatta acatcaatta taaataaagc
    13801 agttttactc aaatcataat tttctatttt ttctattggt agattatcta aattactctt
    13861 taaagcttca agttcattta ataagttatc catagttata cctccaagat aaatttaata
    13921 aaattgtaca tattaattat ataaaagtca aggagtacaa actattgtat aattagctgt
    13981 attgttttga gtgtaaaaga tttgaagcaa tatctggata gttagtaaaa ataccatcta
    14041 cattgagttt tagacagtgt agtatatcac cctcttcatt tacagtatat acattgacac
    14101 caagtagatt tttgtgacaa agttcaatta gagattcatt tactaaaact agaggtggat
    14161 gaagataact attacccaga aatttagcaa aattgactac attgtttata ggtaaataac
    14221 ataataatcc tgtttttata ctagggtcta atttatgaaa gttttttaaa ctattgctgt
    14281 tgaaactaga tataagaact ctatttttca tattatactt taaaataagc tctaaaacgt
    14341 ctttttctat attgggatag tttattttgt cagtttttag ttctatattt aaaataaggt
    14401 ctgtaggtga aattagaatt aatacttctt ctaaagttgg aattttacat tctttattgc
    14461 tttgatatcc ttcaaaagat gagtttagag tttttaattt tcttaaagta aagtctttta
    14521 caaaaccatg tccattaaaa gttctatcaa ccttttcatc atgaataact acaatttttc
    14581 catccttagt tctgtggaca tcaagttcta ttccatatat atccatatcc aaacattttt
    14641 taaaagctag aatagtattt tctggatact ttccactaaa ccccctatga gcataaatat
    14701 tcatttatat ggttccctcc tataagt
//
LOCUS       DBGJFU010000093        14698 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.093, whole genome shotgun sequence.
ACCESSION   DBGJFU010000093 DBGJFU010000000
VERSION     DBGJFU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.093"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            482..1390
                     /gene="trxB"
                     /locus_tag="ACOIAS_002679"
     CDS             482..1390
                     /gene="trxB"
                     /locus_tag="ACOIAS_002679"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGZ2702381.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1480..3303)
                     /gene="typA"
                     /locus_tag="ACOIAS_002680"
     CDS             complement(1480..3303)
                     /gene="typA"
                     /locus_tag="ACOIAS_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HGZ2702382.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3515..3757
                     /locus_tag="ACOIAS_002681"
     CDS             3515..3757
                     /locus_tag="ACOIAS_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702383.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3905..4096
                     /locus_tag="ACOIAS_002682"
     CDS             3905..4096
                     /locus_tag="ACOIAS_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HGZ2702384.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4387..6888
                     /locus_tag="ACOIAS_002683"
     CDS             4387..6888
                     /locus_tag="ACOIAS_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGZ2702385.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQNVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7048..7716
                     /locus_tag="ACOIAS_002684"
     CDS             7048..7716
                     /locus_tag="ACOIAS_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702386.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7700..8977
                     /locus_tag="ACOIAS_002685"
     CDS             7700..8977
                     /locus_tag="ACOIAS_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2702387.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9378..9599
                     /locus_tag="ACOIAS_002686"
     CDS             9378..9599
                     /locus_tag="ACOIAS_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702388.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(9888..10652)
                     /locus_tag="ACOIAS_002687"
     CDS             complement(9888..10652)
                     /locus_tag="ACOIAS_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGZ2702389.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            10926..11600
                     /gene="fsa"
                     /locus_tag="ACOIAS_002688"
     CDS             10926..11600
                     /gene="fsa"
                     /locus_tag="ACOIAS_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGZ2702390.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(11722..13116)
                     /locus_tag="ACOIAS_002689"
     CDS             complement(11722..13116)
                     /locus_tag="ACOIAS_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGZ2702391.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(13194..14540)
                     /locus_tag="ACOIAS_002690"
     CDS             complement(13194..14540)
                     /locus_tag="ACOIAS_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2702392.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         5816 a         1783 c         2286 g         4813 t
ORIGIN      
        1 aaatagtgct aatatttaaa ctattaatca taattggttc aacaaaagta tatagttaat
       61 aatgtttaat ttgaagaaat tgttagtgtg aaattacata tatattcaaa ttaaattcat
      121 aaatatttca cataataata gtatactgta aacaaaagga ggtattaaat atgggaatag
      181 tacttataac agcagcatta gttttggtta tgtatggact ttcaattcta tcatatatat
      241 cagaaagcaa tatattagaa gacactgtaa atgataacca ataaggtttt gaattatata
      301 aataagtgag aactgtaaat aaaagttaat ttttatttgc agttttttta tatatatgta
      361 aaataaagtt aattaataaa aaataatgtt ttgtttatag tatatatgga taataatagt
      421 aaagtataag aatgatgaga ataatatata agaaataata atataaagag aggtaaacgt
      481 tatggtagat atcattgtaa ttggtgctgg accagcaggt ttaacttcag caatttatgc
      541 tatgagagca gggttaagcg ttacagtatt tgaaaaaaat atttatggag gtcaagttgc
      601 aagtacctct gaggtagaaa attatccagc agttcaaaag atttctgggg tagaattttc
      661 taataatata tacaatcaag cagttgctca aggtgttgat attcagtttg atgaggttga
      721 agagataaat ttagagggaa aagtaaaggt tgtaaaaact tcttcaggtg aacataaagc
      781 aaaggctgtt attcttgcaa atggagttga aagacgtaaa ttaggttgtg caggggaaca
      841 agaatttaca ggtagaggag tatcttactg tgcaacatgt gatggagcct tttttaaaga
      901 taaagaagtt gctatagtag gtggaggaaa tactgcacta gaagatgctt tatttttagc
      961 taataattgt acaaaagtgt atttaattca cagaagagat agttttagag gtgaagaagt
     1021 gttggagaaa tctgtgaaag caagagagaa tattgagata ctttacagtc atggtgtaga
     1081 aaaaatagaa ggagaaaaga ctgtatcgaa aatagaagta aaaaacttaa agactgaaga
     1141 aaaaagaact atagatgtta gtggtatttt tattgctata ggtcttaaac ctaataataa
     1201 aatgtttgaa aatgttcttg atttggatga aggtggatat ataatttcag atgagtcttg
     1261 tacaacatca gtagaaggtg tatatgtagc aggagatagt agaactaagt ttttacgtca
     1321 aataataaca gcagcatcag atggagctat tgcagccgtt caagcagcta attatataaa
     1381 tgttgaataa tataaatttc aaataaaaat agccatataa actgaaataa tttttcagta
     1441 ctatatggct attttgttaa ttagaggtaa atgtacgttt tatttacctt gtctagattt
     1501 gttatatctt aatctatcat gttcatttaa gaatcttttt ctaagtctta tagaatcagg
     1561 tgtaatttca actaattcat catcttctat aaactctaga gcttcttcaa gagtaaatat
     1621 tctaggagga gataatttta tagcatcatc tgaaccagaa gcacgtacgt tactcatttt
     1681 cttgttttta caaggattga ctaccatatc atcttttctg gaattcatac caattatcat
     1741 accttcgtaa acttctacac cagggtctac aaacattaca gctctatcac ttaaggcatt
     1801 aagagaatac cccatagtaa caccaggtcc ttgtgctatt aaaacaccat ttcctcttga
     1861 aggtatttct cctttaaatt cttcaaattt ttcaaatgaa cgaactaaag taccttctcc
     1921 acgagtagcg tttataaatt cacttcgata tcctaaaaga ccacgagtag gtgctaagaa
     1981 ctctattttt acatagtctc cttctattga catagactcc atcatacctt ttctcatgtt
     2041 taattcattt ataatagtac cagagtaaac ttcaggacag tttacaacaa ctctttctat
     2101 tggttccatt agttttccat cttctttatg cattaaaact tcaggtttag aaacaccaac
     2161 ttcataacct tcacgtctca tattttcaag tagtatagat aagtgaagct ctccacgtcc
     2221 agaaactttg tatccatcag tagtatcaag aggttcaact ttaagaccaa catttacttc
     2281 aagttctttt tcaagtctat ctttcaaatg tctagttgta acgaattttc cactttttcc
     2341 aacgaatgga gagtcgttta ctaagaagtt catagaaaga gtaggctctt ctatttttat
     2401 catttccata ggtagtggac tatctaaatc gcatatagtt tcacctatag atatatcagg
     2461 aataccagct ataacaacta tttctccact agtagcttca tcaacttcaa cttgttttaa
     2521 accttcatac acagaaagtt ttgatacttt tcctttagat acaacagaat cttctctaca
     2581 tatagcaact tgtgtattgt ttttaagagt acctttgtaa attctaccta taccaagtct
     2641 acctacataa tcatcatatg caagagctga tatttggaac tgtagaggtt cattatcgta
     2701 attaggataa gcttcaacgt gatttacaat agttttaaat agaggagaaa ggtcttcact
     2761 atcatcatcc atttcaagtt tagcaatacc ttgtttagca ataccatata ttataggaaa
     2821 ttcgcattgc tcatctgttg catttaagtc tacaaataaa tcgaaaactt cattaactac
     2881 ttcttccgct ctctggtctt ttttatcaat cttgtttata aataatattg gttttaaacc
     2941 gaactctaaa gatttttgta aaacgaatct agtttgaggc attgggcctt cactagcatc
     3001 aactaataga ataactgtgt caacagtttt cataacacgt tcaacttcag aagaaaagtc
     3061 actgtgtcct ggagtatcaa ctatgtttat tttgtaatct tcatagttta ttgcacagtt
     3121 ttttgaatat atagttatgc ctctttcttt ttcaaggtcg ttactgtcca taacgcagtc
     3181 tttaactact tcgttctttc taaaaacacc gctttgagaa aggaaagcat caaccaatgt
     3241 tgattttcca gcgtcaacgt gggctataac tgcgatattt ataattttat gtttttgtga
     3301 catttttgtt gctcccttcg attaaaataa cttattaatt atagcacaat atattattag
     3361 gtacaaggag gaatatatta aattgtatta atatagttta aattggataa aactgttagt
     3421 tttgcatctg aaaattattc tgtttaaaaa aattttttag acaaataata accttaatag
     3481 ataaaaagat taataaaaga gtaaaggatg atatatgttg gaagtaacca aaataataat
     3541 taaatatttt gcaagtgaaa gtgatacttt tgtaaaagaa gaagtttgta attttgaaga
     3601 acttcttaag tggtctcata aatttattga aggtaaaaag atagaagatg ttttgtatat
     3661 aaagtctaaa gaaaactata aagtaaatgt gattaagtct ttagaaatta aggaattgga
     3721 aatagtgaga aaacaacaga taccaccgta catgtagtat taataatata taaaattaat
     3781 atgtaatact cgcaatagga tataaatggt atatatggta ataatttata ttaaaaaaac
     3841 ttttataaag tttatatttg ggtataaaag tattaattag acattaatta ggaggaactt
     3901 aaatatgata agagtatgtc caaactgttc aaatgtggat gtaaataagc taaaaacttt
     3961 ggttggagaa gaaaatgtaa agactggttg tataggtcag tgtagagcat ttaaaaaaga
     4021 agctgttgga ataatagaca atgagcttga aataaaggaa acagaagcag agttttttga
     4081 agcatgtaaa gaataaattt taaataaaat ataaattata ataaatagag catctaaatt
     4141 gtataagcat taaacaattt agatgctcta tttttatata tacatttaat tatgagtata
     4201 cttttctatt aaatacaaat aaatataact aatacattta tttattaaaa aaatattgat
     4261 ttaatatacc tatagggggt ataatgaaaa tatagatttc taaataagta atttatcaat
     4321 attacatatc aaaataaaac gttacaaatt agctgaaaat atattgaaag aaaggaagat
     4381 tttattatga gttcaaataa agtggtagaa agttataaaa ttacaggtat gacatgtgca
     4441 gcttgtgcaa aggcagtaga gagagtaact aagaagatgg atggagtata tgaccaaaat
     4501 gtaaatatag ctactgaaaa acttaaaata gaatatgata attctaaggt taattttgat
     4561 gatataaagc aggttgtaga aaaagcagga tatggaatta taaaggaaga aagtaataaa
     4621 aaaattgata tgaaaattga tggaatgaca tgtgcagctt gtgcaaaagc tgtagagaga
     4681 gtggtaaaaa aattggatgg agtagaaagt attagtgtga atattgcaac agataaagct
     4741 aatatagact atgacccatc aaaagtaaaa ttatctcaaa taaaagcagc tattgaaaaa
     4801 gctggatata aaccaataga agaggtaaga aataaggttg atgtggatga agataaattg
     4861 agaaaagaaa gagaaatgaa gtctttattt gttaaattta tagttgcaat agtatttgca
     4921 gtacctctgt tttatatagc aatgggacct atgataataa agcctatagg gccatggcct
     4981 ttgccagaaa ttataaatcc tatgacaaat acttttaatt atgcattaat tcagttaatt
     5041 ttagtaatac cagtaatgat agctggttat aaattttata taaatgggtt taaatcactt
     5101 ttttctttaa gccctaatat ggattcttta gttgcaatag gaacattagc cgcattttta
     5161 tatagtctat atacgacact acaaatagcc aatggacaaa ttcaaggtat gcaccatcat
     5221 cagttgtatt atgaaagtgc aggaataata atagcactta tcttacttgg taaatattta
     5281 gaatcaaaat caaaaggaaa aacatcagaa gcaattaaaa agcttatggg gctacaacct
     5341 aaaactgcaa tagtgttagt tgatggtaaa gaagttgaaa ctcctataga agaagtagag
     5401 ataggagata tactattagt aaaaccagga actaaaatac ccgtggatgg agttgtaata
     5461 gaaggatata catcagttga tgaatcaatg cttacaggtg aaagtatacc agtagaaaag
     5521 aatgttggaa gtaaggtgac aggagctagt attaataaaa atggtgttat taagtttaag
     5581 gctgaaaaaa ttggtggaga tactgctctt gcccaaataa taaagttagt tgaagatgca
     5641 caaggtacaa aagcacctat tgctaaactt gccgatactg tatctggata ttttgtacca
     5701 atagtaattg caatagctgt agttgcatca ttattgtggt ttttaatagg aggaaaagat
     5761 atagtatttg tacttactat atttatatca gtacttgtaa ttgcttgtcc ttgtgcatta
     5821 ggattagcaa caccaactgc tataatggtt gggactggaa agggcgcaga aaatggaatc
     5881 ttgataaaag gtggagaggc actagaatct gcacataaag taaatacagt gatatttgat
     5941 aagacaggta ctataacaga aggaaagcca aaagtaacag atatagtatt aaataataat
     6001 gtaaaagaag aatatcttat caagattgct tcaagtgcag aaaaaggttc agaacatcct
     6061 cttggagaag caatagttaa gtatggagaa gagaagaata taaaatttga aaaagttgat
     6121 aattttaaag ctataccagg tgctggtata caagttacta ttaatgatga aagtatatta
     6181 cttggaaata gaaagcttat gaatgacaat aatataaagc ttggagattt agaagaaaaa
     6241 tcaaatatat tggcaagcca aggtaaaaca cctatgtata tagctgtaga tggaaattta
     6301 tcaggtataa tagcagttgc agatgtagtc aaagaaagta gtaaaaaagc aatagaaata
     6361 cttcatgata tgggaataaa agttgcaatg gtaactggag ataatgcgaa aactgcaaat
     6421 gctatagcaa atcaagtcgg tatagatatg gttttggcag aggttttacc agaagataag
     6481 tcaaaagaag ttgaaaaact acaaaatcaa ggaaagtttg ttgcaatggt aggggatgga
     6541 ataaatgatg ccccagctct tgcaaaagca gatataggga tagcaatagg aagtggaacg
     6601 gatgtagcca ttgaatctgc ggatatagta cttatgaaga gtgatttaat tgatgttcca
     6661 acagcaatta aacttagtca tgaaactata aaaaatataa aacaaaacct attttgggca
     6721 tttggatata atacaattgg tatacctgta gcagcaggaa tattatatgt atttggagga
     6781 cctttattaa acccaatgat tgcagcagca gcaatgagct taagttcagt ttcagtcgta
     6841 tcaaatgcac ttagacttaa aaacttcaag gcatataaga gagattagat taataaatta
     6901 aataaaatgt atccaaaaga gctatcttta aattaaaagg attaatttac aaattaagat
     6961 agctcttttt tcatgtaaaa ttcacaacaa taagataaaa tataagtaat atacgaaatt
     7021 ttaaataaaa tagaaaggat tgatagaatg aattcgtcaa tacttgtaat tgaagatgat
     7081 tcaaatatac aagagttaat atcagaattt ttaagtgcag aaggttatca agttgataca
     7141 gcaaatgatg ggttggaagg tatacaaaag tttaagcaag gaagttatga tttagtaata
     7201 ttagatataa tgatgccaaa cttagatggt tatggagttt gtaaaatgat aagaaagtca
     7261 tcaagcgttc caattatatt tttaactgct ttaaatgatg aaggagacca acttaaaggg
     7321 tttgacttag aatgtgatga ttatataaca aaaccatttt catttaatct tcttataaaa
     7381 agagtagaag caattttaag gcgaagtaac aagactataa atgacaaatt tatagttttt
     7441 gaaaaattaa aattagattt aaacacatat attgcagaga tagatggaga acctatagaa
     7501 cttacactaa aagagtttaa tatattaaaa gctttgatag aaaagtatcc acaagttata
     7561 actagggaag gtcttttgga tagtatatgg ggttatgact attatggaga tactaggatt
     7621 gttgatgcac atataaaaaa tataagaaaa aaaatatcat taccatatat taaaactgta
     7681 aaaggaatag gatatacact tgagaaggat atttgataag tgggaaaagt taagtataaa
     7741 gtataagtta ttttcaataa cgacatcatt attaatagca ttagctttaa taatatatct
     7801 aattttatat tttttattgc catcatatta tcatgagtat aaaattgagt ctcttcaaga
     7861 aagtttgaaa tctttagtag atagttcaat tcatttcgat acatatactt tagaagaaag
     7921 actttattat atggctaaag accaaaattt ggctatttta ttaaaagata accaaggtaa
     7981 aattgtatat ggtaagaatg aagtagttat tttaagatat agtaagtaca tgattaatag
     8041 tcttgaggat gagtatagaa cttctatacc tatatatact aaagatgcaa aagatggtcc
     8101 atatacttta gaactcgtta tgccattaca accaattgac gaagcaaacg aagttataag
     8161 aaaactaatg ccatacatca tatcaatagc aatattgata gcgattattg gagcatatat
     8221 atattcaatt gtaataacaa agcctcttat aaacataata gagagtgaga gagaacagga
     8281 atatagaagg aaggattttg ttgctacaat atcacatgaa ctaaaaactc caataaccat
     8341 aataagtggt caaattgaag gtatgattta tagtgtaggt aagtataagg atagggatac
     8401 ttatctgaaa aaatcgtatg agtgtacaca agaattaaaa gatttagtaa atgaaatgat
     8461 agaggtttca aaatcagaaa tacttgaaaa agatttgaaa ttggtatcaa taaatataag
     8521 tgaattatta aatagacttg taaagagaca agtattttta atagaagaaa aacatatgaa
     8581 aactatttta aaaatagaag aaaatttgga aattaaagca gaccaagaga gaataactaa
     8641 ggcaatcaat aatataataa ataatgccat aaaatattct cctgaagaat cagagcttat
     8701 aattagactc tatgataaaa ataaacgaat aagtaaaaag acttctaatc aaagagtggt
     8761 tttagaaatt gaaaatactg gtgtaactat cgagaaacgt tatttagaag aaatatttaa
     8821 tccattttat agaatagaaa aatctcgttc aagaaaaact ggaggaagtg gacttggact
     8881 ttatatagtc agccaaattt ttaaaagtca tgggtttgat tatagcataa aaaacaaaga
     8941 aaactctgta gtattcacag tagaatttaa aaattaatta cttattataa agaatatata
     9001 actttcaata atagttcatt ttaaaatcac aaaaaattca taatgaaact gtattatata
     9061 aatataaaga agcttaaaac cataaaatac accaactata atgatgagat ttccttacat
     9121 cctccatata agtcatcatt atagtaaccc aacaattaag ttactatgaa tttaaaaaat
     9181 aacaccgagc atataatcta tgcttagtgt tatttttatt ttgaaataaa aaatatttaa
     9241 tttattgaaa tgtatagatt tagatagttt aaactatatt taagacttaa aaattataat
     9301 tgatattaca aataaatata tgtactttgg gaaaaataaa tataggaagt attatattta
     9361 cagaggaggt agttttgatg gatagattaa aaagagatat gaaaaatatg aaagaagatt
     9421 taaaagaagg tgttaaaaag acagcttatg ttatgaaaaa tacagctgaa gatttgaaag
     9481 atgatgcaca aggtgctatg gtaaacttag aaatgaaaaa agatgagatg atggaaagat
     9541 atgaacaaaa gaaattttct aaagaattag agaagcaaat gaagaatgaa atgaaataag
     9601 cttttattta tgaataacaa attattgtat tcatataaaa agtagccagt aatattaaat
     9661 agtttattta aggaaataga ctttctaaaa ataactgtat tagatttaga aagtctattt
     9721 caaattagtt ttataaatag acataaggat aaagtagatg ttatagaact agattttaaa
     9781 aaatagatac taaggataaa ataaatatta tagaaatatg tttttaagaa tatttatcaa
     9841 ataaaaattt atatttataa gaaatcctat aaaacatata attaaactta taaaacattt
     9901 acattaaatt ctgataaaat atctactata tcaggagtag gcgttttttc tgtaattata
     9961 gaactaatat cttctaaatg agcaaacttg aaatttccat caaatttaaa ctttttatct
    10021 tccataacta taaatacttt tttacttgaa tttattatag ccttcttcgt aagaccatct
    10081 tcaatttcaa aagttgtaag tgaagagttg aatacatcta ctccacaact tcccataaaa
    10141 gtcttatcaa acttatattt cgatataaac tctatagttg gagtaccgac aaaaccgtct
    10201 aaactgagat ttaataaacc tccagtggaa attatattta aattatttgg ttttgaagta
    10261 actacattta gtatatccag catatttgta acaagtgtta cttttttatt actttcagct
    10321 aacaacttcg caagcaagat gttaattgta gagatatcta aaaatatagt ttctctatct
    10381 tcaattaaat taaatgcttt ttctgctatt attttctttg ttggaatatt aatttctttt
    10441 ctgtcttttg tatcctgatt ctctagtgtt tctctagcta gtacagctcc tccataaatt
    10501 cttcttattg aagaaatctt ttctatattt tttaaatctt ttcttataca atcttctgta
    10561 acattaaatt tagaactgag ctctttgaca gtaactctac cttctttttt taggatattt
    10621 aatatagctt ggattctttc ttctgcaaac attttctacc tcttttcgca atatagtatt
    10681 atagtaaata ttataagata aaatacaata tatcaactat tttaacatga aatacaaaat
    10741 taatttaata taaaaagtat gttttttcta actatatatt tagaatttag taaataaaaa
    10801 aattataaaa ctaagaagga aaagtaaaac aataataaaa aataaaaaac aataagcaaa
    10861 atgttgaaaa taataccaaa tcaatatata ataagaataa agaaagatta aagggggata
    10921 ttttaatgca actaatctta gatacaggta atgtggagga gattaaggag ttatgtactt
    10981 gtttaccaat agatggtgta actacaaatc caagtattgt ttcaaaagaa aaaaagaatt
    11041 ttaagcaact tataaatgaa attggtgaga ttataggtga ggatatgcct atacatgctc
    11101 aagtgttaag tacaaagtat gaagaaatat tagaagaagc attatatata agtagtttga
    11161 gaaaaaatat atatgtaaaa atacctgtaa cacaagatgg tttaagagct ataaaagacc
    11221 ttcatagaaa aggtgtaaaa ataactgcta ctgcaatatt cactgctcat caaggttttt
    11281 tagctgcaaa ggctggagca aattatattg caccttatgt aaataggctt gataatatat
    11341 ctggtgatgg agtagctatg gtttcagaac ttataaaaat aatagatact tataaaatgg
    11401 atacaaaagt acttgcagcc agttttaaaa atgcacaaca agttatagaa ttaatgcaac
    11461 atggggtgca tagtgttaca gtaccatatg atatttgtaa atctatgatg aatcatccat
    11521 taactgattg gagtgtagat aagtttatag aggattggga gaatactttt ggaaaaggaa
    11581 gtaaaacaaa taatatttag tattatggta aaagagtagt tttgtccaat tttacaagaa
    11641 cttatataaa aagatgaaaa tatgaatttt ctttttaata atttcgttat tatcttaagt
    11701 taattcatat tttcatcttt tttagatttc tagtcctata aatttccacc aagggaatgt
    11761 tatacatatt attataaagt aggttaaagg aatcaaagtt aaaccgtatt tgaaaataac
    11821 cttgctatta tacagatttt caccatgacc aaccattatt gtagcatgat gaaatggtaa
    11881 tatgtactgt atgcttacta taatgtagac aaatagacat aaagtaacac tattttcctt
    11941 tagtatatgt aaacttccaa gtaatggtat tactacagat gatgtagtga cagcacttcc
    12001 aagacatata ttcaagatta ttgtgagagt tatcaaaaac aatattgaaa taatttctga
    12061 agagtttggt ataaacttta tcaaataatt tcccattata tctacaactc cagagtactt
    12121 taacactcct ccaattgaaa atgctgctat aaaaaaaata aggaccttcc aatttatttc
    12181 ttttaaagta ctaaatccaa ctatttttct tagtaataaa agaagtacac aaattgacat
    12241 tataattaat gtgtttatgt tatgtatgaa ctgagttaaa aacataaaaa acattattcc
    12301 taggcataaa aatacgtgtt tttgttcttt gtttagcaaa ttaaattctt tttttaaatt
    12361 agaatatgaa aaatgaagtt tattaccctc aagattttca ttagaaataa attttattga
    12421 atgcaattct tttttaaaga caaaggtaaa taaaaaataa gttataaaac aagtaattat
    12481 agttggaaca gacatataaa aagcccattg accccaattt atattacaat ttccaagttc
    12541 tagtacaaca taatttagaa gagtgtctcc atttacaaaa aacattgatg taaaggtaga
    12601 tgctgtaaat atactaaata aaagtataga ttttgaatct tcagttatct gttgttcttt
    12661 taaaaactcc ttgtaaaaag cagatacaag aataaccctt ggaaatggtt gtgggataaa
    12721 aaatattaaa agtataccta aaatatacga taaaagaatc atttttttag gggtatttac
    12781 agtatttaaa attaacttct cagaaataat ttttgcgaca cctgtttttg taactgcatt
    12841 agtaattatg tatgcaagaa ttattatgta aaaattttct gtaaataaaa actgtaaaat
    12901 aacctttatt ggtgcaatgt taaatataaa cgctagaatt acaaaaaaca atgcaacaaa
    12961 agttttttca actgagtcta cagcccatat caagatagtt aaaaaagata agcatataga
    13021 aaattttatg ttgaaactgt aattaagaga gaagtagaga attatggtag ctataacagt
    13081 catataaaat tgttttggct taagaataat acccattttt atattcacct ccataattta
    13141 ttatatcata atggaggtga atacaatgaa tgtgaataca ttttataaaa agattattca
    13201 ttgattctat gatttacaat agctttagtt ttccaatgtc ctttcttata gtagataact
    13261 gtaacaataa gacctaaagt ccaaccaaaa ggaataccca tccaaattcc gtctgttcca
    13321 aaaaatttag aaagcatata agaagctgga agtcttccaa gccataaaga tactattgag
    13381 cttataagtg caaacatgga atcaccagca cctttcattg cacttgaaag tacaaaagaa
    13441 gttccaataa ataagaagaa tggtcctatt ataaataagt aagaagagcc aattttaatg
    13501 acatctttat cagtattgaa tagagcaatt agattttccc taaatagcaa aattaaagca
    13561 ataactgtaa ctgctaaaac taaggtcatt tttatagatt ctcgaatgcc ttttttaact
    13621 ctttcatttt ctccagcacc tatgttttga gcaacaaaag tagaaacagc agaaccaaga
    13681 ttcataatag gtaagcttat aaaattttct atcctaagac cagcaccaaa agctgccata
    13741 gcagatgtac cataactatt aactaaaaat tgaagagcca tatttccaat tgaaaacaac
    13801 atttgttgaa ctccacttgg aagtcctaat ctaagtccat ctttaaatat tttgttatca
    13861 tatacaattc ccttgatttt aaatttaagc acttcatgtt ttttatttaa ataaattata
    13921 cttattataa atgatactgc ctgagccatt atagtggcaa gtgcaacgcc ctcaacaccc
    13981 attcttagca ctacaataaa agttaagtct aaaactatat ttagaattgt ggctattatc
    14041 agaaaataaa gtggagttac tgaatctcca agtcctctaa gtattgcact tactgaatta
    14101 tatccagcag aaaataatat tccactaaat attatagtaa gatacaattt tgattgagca
    14161 aaaacggatt ctggtgtatt cataagctta agcattgggc caccaaatac tattccaaga
    14221 atacttatta atatagaacc aataaataaa agtatatatg tagtatcaat agtagctttt
    14281 acctttttta aatctcctga gccataaaat tgtgacacta aaatagaaaa tccaaggcta
    14341 acacccataa gcgctgccac caataaaaac attatagggt tagctccaga gactgccgcc
    14401 attgcttctt ttccaataaa tctaccaact attatactgt cagctgtatt gtatagttgt
    14461 tgaaataaac taccaataag cataggcatg gcaaaaaaga atatggactt tccctcatga
    14521 cctgtagtta aatctttcac cttattcctc caaataaaaa aattattaat ttattatagt
    14581 ataatcttaa cataaacttt atatccaaac tataaataaa ttgtatcaaa atatcatata
    14641 tttttttaaa atgtaaagaa tataattata atttgaattt aataaatcaa tatttaat
//
LOCUS       DBGJFU010000094        14691 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.094, whole genome shotgun sequence.
ACCESSION   DBGJFU010000094 DBGJFU010000000
VERSION     DBGJFU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.094"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..1032
                     /gene="nth"
                     /locus_tag="ACOIAS_002691"
     CDS             <1..1032
                     /gene="nth"
                     /locus_tag="ACOIAS_002691"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGZ2702393.1"
                     /translation="IEKKEINKKGIDKKDIIEKKEINKKEMDKKDIIEKEEINKKGMN
                     KKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEK
                     KTKKSSSNKATQKTSKETKSKFKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAF
                     ELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGIYKSKSQK
                     IKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSN
                     RIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCN
                     YYKELNETK"
     gene            1226..1696
                     /gene="trmL"
                     /locus_tag="ACOIAS_002692"
     CDS             1226..1696
                     /gene="trmL"
                     /locus_tag="ACOIAS_002692"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGZ2702394.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            1732..2580
                     /locus_tag="ACOIAS_002693"
     CDS             1732..2580
                     /locus_tag="ACOIAS_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGZ2702395.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKKLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            2919..3923
                     /locus_tag="ACOIAS_002694"
     CDS             2919..3923
                     /locus_tag="ACOIAS_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2702396.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            4049..5161
                     /locus_tag="ACOIAS_002695"
     CDS             4049..5161
                     /locus_tag="ACOIAS_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2702397.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATEYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            5651..6412
                     /locus_tag="ACOIAS_002696"
     CDS             5651..6412
                     /locus_tag="ACOIAS_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6873 family GME fold protein"
                     /protein_id="HGZ2702398.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            6415..7233
                     /locus_tag="ACOIAS_002697"
     CDS             6415..7233
                     /locus_tag="ACOIAS_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HGZ2702399.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            7413..8015
                     /locus_tag="ACOIAS_002698"
     CDS             7413..8015
                     /locus_tag="ACOIAS_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HGZ2702400.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    8474..8711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8775..10694
                     /gene="thrS"
                     /locus_tag="ACOIAS_002699"
     CDS             8775..10694
                     /gene="thrS"
                     /locus_tag="ACOIAS_002699"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGZ2702401.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            11462..12553
                     /locus_tag="ACOIAS_002700"
     CDS             11462..12553
                     /locus_tag="ACOIAS_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGZ2702402.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            13223..14563
                     /locus_tag="ACOIAS_002701"
     CDS             13223..14563
                     /locus_tag="ACOIAS_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HGZ2702403.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         6002 a         1352 c         2620 g         4717 t
ORIGIN      
        1 attgagaaga aagagataaa taaaaaagga atagataaga aagatataat tgagaagaaa
       61 gagataaata aaaaagaaat ggataagaaa gatataattg agaaggaaga gataaataaa
      121 aaaggaatga ataagaaaga tataattgag aaggaagaga taaatgaaaa agaaatagat
      181 aaaatgattg gtaaaaaaga tgagataatt gatgagaaag atatagagaa ttttatggat
      241 attgaagaag ataaaaaaag tatcaaagta agtaaaaaaa gtactaaaaa aggtgaaacg
      301 gagaaaaaaa caaagaagag tagttctaat aaggctacac aaaaaacaag taaagaaact
      361 aaaagtaagt ttaaaattaa aaatgagtca gcaaatgaaa aagacgtaaa taagatttta
      421 gacgagttag aaaaactgta cccagatgct aaatgtgagt taaattatgg gacagccttt
      481 gaacttttaa tagcaactat tttatcagca caatgtactg atgtaagagt gaataaagtt
      541 acttcagagt tgtttaaaaa gtacaacaca gctagagact ttgctaattt gagcattgaa
      601 gaaattagta aagagataaa gagttgtggt atttacaaaa gcaagtctca aaaaataaag
      661 gatacatctg aacagctatg tgaattatat gatggtgaag tacctgatag cttagaaaag
      721 cttataaaat tacctggtgt tggaagaaaa acggcagggg ttgtacttag taacgctttt
      781 aatcatccag caatagctgt agatactcat gtatttagag ttagcaatag aataggaata
      841 gtggacgagc caaatcctca aaaaacagaa tttgcactta tggaagctat accaaaagaa
      901 agatggtcac actcacatca tgtattaatt tttcatggaa gaagaatgtg taaagctaga
      961 aatccagaat gtgcaagttg cccaataaaa gaagattgta attattataa agaacttaat
     1021 gaaacgaaat aacttttaga aagtaagttg tacaagaggg attttctgat ataataatac
     1081 ttgtgaaaat gttagataaa aaactattat atacatgaaa taaaaattat tattaacatt
     1141 attttatata atagtataat attattgtta ttctgtttat aaaataaaat ttgataaata
     1201 aaagcgatac ttagggagga ttaaaatgtc attaaatata gtattagtag aaccagaaat
     1261 acctcaaaat acaggaaata taataagaac ctgtgcagca acaggttcaa ctttacattt
     1321 agtaaaacca ctgggttttt cattggatga taaacaccta aaaagatgtg ggcttgacta
     1381 ttgggatata gctgatatac aatattacga tagctttgaa gaattacaag aaaagtatcc
     1441 aaatagtaaa tacttttttt ctacaacaaa ggctaaacag tcacattcag atgtgaaata
     1501 tgaagaaaat tgttttatag tatttgggaa agaaacaaag ggattaccag aacctttgtt
     1561 gaaagcaaat atggatactt gtattagagt gcctatgttg gatgttgaaa aagctaggtc
     1621 attaaattta tcaaattcag ttgctattgt agtgtacgag gctttaagac aaataggata
     1681 cccaaatatg aggtagtttc aacaagccta aatgagaaag gagcatactt aatgaaaaat
     1741 ctaaagataa tctgtgacag tttatcggac gtgccaaaag atttgttaga taaatatgat
     1801 atagatatag tttcattaac agttatactg gaggaaagag aatacaagga tagaattgat
     1861 atatctggag atgaatttta taaaaagctt agagaagaaa atgtatatcc aaaaacttct
     1921 caagctactt atgcccaatt taaggaaatt tttgagaaat atacaaatga ggatagagag
     1981 atactttata tagcagggtc tgccacagct acaggtacgt atcaaagtgc tgtaatggca
     2041 aagaatgatg tagatggtaa aatatataca tatgactcta acttgctttg tatgggaact
     2101 ggtttacttg taataaaagc aggagaactt gcaagtgaag gaaagtctgt tgctgaaata
     2161 ttgcctgttt tggatgaatt aaaagagaag gcttttgtta tattttcagt agatacattg
     2221 gaatatttac aaaagggagg tagaatatca tctactaagg cagcaatagg aagtatatta
     2281 aatataaaac cgattcttga aataagagat ggattagttt cgcaaatatc tcaagtaaga
     2341 ggaaagaaga atgtaatttc taagatgatg gaattgataa aatcaaattg tggtgatgat
     2401 ttatcagacc aaattgtata tataggatat agtgatgatt ttaaggaaaa agaacaatta
     2461 tctaaggtta tgcaagatga attaagacct aaagaaatta gatttttcca aattggtgtg
     2521 tgtataggtg cacatgctgg tcctggtgtt actggtatca tatgcttaaa aaataaataa
     2581 tatatttttg gatgacatta tttatttttt tattaaatta ggatagaata ttaattattg
     2641 agatgttaaa ttggatttat tttggatgaa attaagtgtt gatatattag atttagtttg
     2701 tagggaagtg tgtaaataga taagaatcta ttgcacactt ttttatagta agatttatta
     2761 aaaaaatttc aaaatgtact tgtaaattgt atataaaaaa attataaata ggatgtaaaa
     2821 gtaaatagga tgtaaaagta aataggatgt aaaagtaaat aggatgtaaa agtaaatagg
     2881 atataaaaat atcgtaaatg tatgaggagt gaaatttaat gcgtttcaaa agaatattga
     2941 tattgatgtt aactgtagca atgatagctg gaatgttggt tgggtgtgct aataacccaa
     3001 gcaataatcc tagctctagc aaagataata aaagtgatat aagcgataaa agtaattcaa
     3061 aaaaatctgt atcaatgata ctagatattg aaggaacaaa taatgaagct atgaataatt
     3121 cagctttatt agcattaaac aatgctcaaa agaaacttaa tatagataca aacaaagtgg
     3181 aatcagatga ttcctcaact ttttctaata gtatagatat tttatgtaat gacaattacg
     3241 atttaattat agctgtagga gctagatttg caaaaccatt ggagatggta gccaagaaat
     3301 atccaaagca acaatttgca attatagact atgagtatga caagcaacct tctaatataa
     3361 cttcaatttc ttatgaagat aataaatctg gatatctagc tggattaatt gctggaaaaa
     3421 tgactgaaag cgataaggtt ggttttatag gaggaacaaa gagttctagt agagataaat
     3481 ttgaatcagg ttttagagag ggtgttaaat tttctaatag tagtataaag gatatttcag
     3541 ttgagtatgc agatgttttt aaagatagca aatcagtaga atctatagct aaaaaaatga
     3601 tggataatgg agtggatatt attttttcta ctactgaaga tgattcaaaa gctgtaatag
     3661 atgcagtaag ggcaaaaaat aaaaaagtta tagcaacgaa taaagaccaa catgagttag
     3721 ctccagaaaa tgttataagt tctattgtaa aagattttga gaatcctact tataatctga
     3781 tacaaagttt tgtaaaagga aattataaag gcggaaaagt tatagaaaac acagttaagt
     3841 ctggaagtac tgggcttgcc caaaatagtt ctcaaaatat tcctccagat gtgttagaat
     3901 atgttaataa gaacaataaa taatctacta taatatccaa gaatacttat gactttagtt
     3961 atgaggtgaa ttggatgtta taatagacat atatagaaag acatatgcag atgtaggtca
     4021 gacctatcta aatcagggag tgaattttgt gaaaataaaa aggatattga tggcttgctt
     4081 tgtagtagtt tttacaattt ttatattagc tatatgtaat tttgattttg ggtttgataa
     4141 agtaaataag aataattcta atatagatag tgctaaatcg aaacagagtg agtctaaaag
     4201 tttgccaact tcagcagaaa aggataaatc aaatagtaaa agtaagaata aagaagatga
     4261 cacagaagat gattcatatg taagaaaaac tattggagta ttgttagata ccgaaaactt
     4321 aaataacgaa tcatataata acaatgtact atcagcctta aaaagggctg acgaagattt
     4381 tgatgttagg ataaaacttg aaaaaccaaa gacttctaaa agttttaaag agagtataga
     4441 aaaactgcat aagtatgaac cagatttgat aatagcagtt ggagccagat ttgcagagcc
     4501 aattaaagaa gctgctactg aatatcccaa acagcaattt gcaatagttg actatactta
     4561 taaagttcag ccagctaatg taatttctgt atcatttcaa gataatgtta ctggatattt
     4621 agcaggactt attgcaggta aaatgactga tactgggaag gttggtttta taggaggagt
     4681 tgagggttct agtagagata agtatgaatc tggatttaga aaggggctta aaacttcaaa
     4741 taaagatgct gagttactag ttaagtattc aaatatattt gaaaaccctg aatcagctag
     4801 aactattgca aaggaaatga gagattctga tagagatgtt gtattttcag ctacagagga
     4861 tgatagtaaa agagctattc aggtagcaag agaaaatgga ggtaaagtta tagctattaa
     4921 taaagaccaa tacgatatgg ctccagataa tgtaataagt tcaattataa aagatgtaga
     4981 acaacctgta tatgaactga taaagaacgt tgcaaaagat ggttttaagg gaagtaaagt
     5041 aatggaattt aaaataaaag atggagaact tggcttgact tcaaagagca gtagaaatat
     5101 accaccagat gttttggaat acataaaaaa agagtataac aaagtgaaag atacatatta
     5161 attattgatg tagaagatta taaaaatata ctgcttaagt ttagattggt gataaaagga
     5221 tatatttatt aaatttgtgc taactgtaca aaatatattg attaagttta gattagtgat
     5281 aaaaagatat atttattaaa tttgtgttaa cagtatggaa tatactgctt aagtttagat
     5341 tggtgataaa aggatatatt tattaaattt agtctaactg tacaaaatat attgattgag
     5401 tttagattag caataaaaag atatatttat taaatttgtg ctaactgtac ggaatatact
     5461 gcttaagttt agattagtga taaaaagata tatttattaa atttgaacca actgtacaaa
     5521 gctatactaa ttaagcttag attaactata taaaattata acaataagtg tgtgggcaga
     5581 aaaattctgc tacacacttt atttatattc acataaaaaa tttatcaaaa aaaagaaaaa
     5641 ggagtaaaaa atggaattta caagagaacc atttatacta gatgacgatt tgtgtctagc
     5701 tttggtggac aaaagaatta gttatctaat ggaaaatgaa ttaaaattaa gaaatataga
     5761 gataataaaa acaatagaat gtaaagaatt atatgaaagc ataaaatatc acccggatat
     5821 atgtatttgt aatttaggaa aaggagatat aattgttgcg ccgaatgtat ataatcaata
     5881 caaagagtta ttaagtagat ataaatttaa tataattaag ggagaagcta atttattaaa
     5941 tagataccct tatgatatac cttacaatat tgcgatagtt ggtgactatg ctatacacaa
     6001 ttttaagtat actgacaaag caatattaga ttatattgaa aataataaac ttaaaaagat
     6061 aaatatagag cagggctatt ctaaatgctc aatctgtata gtagatagaa cgtctataat
     6121 aacatcagat aaaggtattt ggaaaagtat gaagaataca gaaatagagt gtttattgat
     6181 agaaaaaggt cacataaatt tatttgagat gaattatgga tttataggtg gatgtacagg
     6241 gttgatttct aaggataaac tagctttttg tggagatgtt aaaaaacatc ctgactatga
     6301 acgtataaaa agttttgtag aaagtaaaaa caaggagata gttacactta gctgtgaaaa
     6361 tttactagat ataggttcta tagttcctct catgacaaga aaggagcgat aactttggtt
     6421 gagaaggata atatttcttc catgaaattt ttgaggaata ataatgtaaa cgatagatta
     6481 atatatacga atgacattgt agaagtagtg atagattctg caaagcaaga agtgttaaat
     6541 aaaaaaatac ttaatggtac taaagctgaa caaagagctt taaatattga tgagatatca
     6601 acggaaataa ctagtatgat aataagtata atgaaagaga aacttttgaa agaatacatt
     6661 ctaaaatgct atgggcgtac atatcctgaa gataaaaata atatatacat atattcactg
     6721 aacatatttg ctaaaaaaga gatttttatg agagaaacag tatttactag ggtgagaaat
     6781 tatatagaag aaaatgattt tataaatgta gaaggtttta ttagatttag aatgagagaa
     6841 tttatgaaat atatttctgc tataggtgat attgcagttg aggagtattt aataaagaaa
     6901 gaccaagatg aatttataag agttttaaaa tactttatag atactcaaga agaaaagatt
     6961 gatttgctta aagttcatat aatggaagat aatacatttg ttttatacga taaaaatgga
     7021 aataagatag acagtataga tgatgaagaa attataaata tggtaataag agaaaattta
     7081 aattatgagg actttttaat aagtacctta ttaactctat gtcccaaaaa aatagaaata
     7141 ttggatttgt taaataataa ttgctcaaag gaaattatag atactgtaga agcaattttt
     7201 gaaaataaag ttagtataat tatggaaaac tagaatctaa gcatataaaa gctatccata
     7261 taaaattaaa aaaattcttt ttaaatcgac acaaaagact tataattgta tataaagcta
     7321 tagtataact agtgttatta agtataaaga atatctactt gattaataca atatagctgg
     7381 caaatataca attgataagg agtaatttta taatggataa tttaattagg atactgatat
     7441 tagctgttat tggtggattt ataggttatg ttacgaatgt agttgctata agactgatat
     7501 ttagaccaat agagcctatt aaaataccta ttttaaatat agaaatagta ggtcttatac
     7561 caaagagaag agcagaaata gcagcaaatg taggagaaat aattcaagaa gaatttcttt
     7621 ctatggacga aatacttgca aatatcatta ctgacgaaga taaggaagaa gttgtaagat
     7681 acataaaagc aagggtaaaa attataatac atgagaaagt ttcatttatc ccgagtggta
     7741 taaaaaacat gatacaagat tatctaggag agataattga gtctgaagtt aagcaaagta
     7801 tagatgaatt gagtaaaaat ataataaata aagcaaatga acgtatagat atacaaaaaa
     7861 tggtcgaaga taagattaat gaactagatt tatatgaatt ggaagagata ataataagaa
     7921 tagctaagaa agaattaaaa catatagagt ttttggggct tgtcttaggg tttttaatag
     7981 gtatagctca agggttaatt actatgttta tttaaatata tgagaagtag catttattta
     8041 agtactaatt atatttacct atttgagtga attacttatt aatagcattt agatattaat
     8101 tgtatttatt tatttgattg tgttacttat taatagtatt taagtattta ctgtactact
     8161 tattaactat attaagtatt gattgtattt aagtatacaa aacctaaaaa gagtacaaaa
     8221 attagaagtt agttttaata aaaattagtt ttagtattta tctagtcata tctatcatgc
     8281 atgtggatac gttttaaaaa ttttaaattt atgaaaatat tataatatca ctataaatca
     8341 agagattaat ttaatataaa aatataagaa tattggttta cagctgattg agatgaaaaa
     8401 taatgggtaa ttaatatatt acattaatgt tgattgatgt ataactatct gttataatga
     8461 aaatatatta aaaatcatag tgttgaagag gaagagtaga gtagaaacca ttctcagaga
     8521 atttgggtct atggctgaga gcccatgtaa taggtctatt tgaaaactac cttggagctg
     8581 agcttattag cagtatttgt tttgtactat acgtgaaagt gctcgggtga cgaccttata
     8641 gtcatcaaga gggttgtata tacttgtata cgccaacttg ggtggaaccg cgaattaata
     8701 tcgtcccaaa catatttggg gcgatttttt attacaaaaa atctaaaaat tatttcaata
     8761 aggaggaaaa aacaatgatt aaagttgctt taaaagatgg ctcaataaaa gaatttgaaa
     8821 atgctatttc tgttatggat gtagctaaat ccattagtga aggattagct agaaatgtgg
     8881 tagcagcgtc tgtgaacgga gaggttgtag gtctggacca cattatagat actgattgtg
     8941 acttgaattt atttaagttt gaagataaag aaggtaagga agtttttaga catacatcag
     9001 cacatatatt agctcaagct ataaaaagac tttacccaga agcaaagctt gctataggac
     9061 caagtataga aaatggattc tactatgata tagatttaga ccatagatta gttccagagg
     9121 acctagagaa aatagaagct gaaatgaaaa aaatagctaa agaagattta aaaatagaaa
     9181 gatttgaact tcctagaaat gaagctttag agcttatgaa agaacaagga gaagactata
     9241 aggtagaact tatatctgat ttacctgaaa gtgaaataat atctttctac aagcaaggtg
     9301 attttactga tttatgtaga ggaccacatt taccatctac taaaaaggta aaagctgtta
     9361 aattacaaag tgtagcaggt gcatattggc gtggtgacga aaataataag atgcttcaaa
     9421 gaatatatgg aacttctttt gagaaaaaca aagacttaga agagtactta catttactag
     9481 aagaagctaa aaagagagac cacagaaaat taggtaaaga attaggatta tttatgatac
     9541 ctgaagaagg tccaggattc cctatgtttt taccaaaagg tatggaactt aaaaatgaat
     9601 tattaaaatt ctggagagaa atacacagaa aagctggata tattgagata gaatctccaa
     9661 tcatattaaa tagaaaatta tgggagactt caggacactg gtatcattac aaagaaaata
     9721 tgtatacagt taaaatagat gatgaggatt atgctataaa acctatgaac tgtcctggtg
     9781 gattaattta ttataactct caactacatt catatagaga tttcccaatg agagttgcag
     9841 agcttggtag agttcataga catgagttat ctggtgcatt acaaggatta atgagagtta
     9901 gagcatttac tcaagacgat tcacatatat tcatgttacc agagcaaatt aaggacgaaa
     9961 taaaaggagt agctaactta atagatggta tatacaaaac ttttggattt gaatataatt
    10021 tagaattatc tacaagacca gagaactcta tgggaagcga cgaagagtgg gaagcagcag
    10081 aaaatggatt aagagaagct ttagaagagt taggtctacc atatactatt aatgagggtg
    10141 atggagcatt ctatggtcct aagatagact tccatctaaa agattgttta ggaagaactt
    10201 ggcaatgtgg tactatccaa ttagatatgc agcttccaag acaatttgat aatacttata
    10261 ttggtcaaga tggtgaaaag catagaccag ttatgattca tagggttgct tttggtagta
    10321 tagaaagatt tataggtata ttaatagagc attatgctgg taaattccca gtttggttat
    10381 ctccaactca agttaagata cttcctatat ctgataaatt tatggattat gcaaatgaag
    10441 ttaagaaaga gttatttgat aagggtataa gagttgaatt agatgataga gctgaaaaaa
    10501 ttggatttaa gataagagaa gctcagcttg aaaaagtgcc atacatgcta atagtaggtg
    10561 aaaaagaagt agctgataat aatgtttctg ttagatcaag agataaaggt gaaataggta
    10621 gtattaaatt agatgaattt atagctagta tttcaaaaga aattgaatca agagaaagta
    10681 ttattcaaga ctaatatata ttatattagt agctaagata ttgttatata agataagttt
    10741 aatcaatatg ttgatatgtt gatgcatagt gattaaattt atgaaaagat gagtataatt
    10801 agaggtacag ttaatcatat ttttatgatt aatgtacctc tagttttttg tatggtagat
    10861 ttattaataa atatatcata gaagtgtttt gaacatggaa atatttatgt gcattcttta
    10921 aataaacgtt tattttttga gtgataatta agtgcttgtt ggtaaggtta tggctgtaaa
    10981 aatcataact ttattttcat atttgttcca ttttcataaa aattattatc cctttaagaa
    11041 aatttcttaa tcctcttgaa atatttcctt tttagtctaa agttaaattt ctttcttcaa
    11101 aatgaatatt taaatgaact aaaatttaag tacattttct aatcttatga gtctattttt
    11161 catagataat tgccctataa tagaagaatc tatgttggtg ctatgtggtg acaataacag
    11221 cattgtcagt gattaattaa tgctttataa atgattaatt agtgttttat gttatttttg
    11281 gacttaccct ctatacatag attaatttat ttaactttag atagagagag aatgtcaagt
    11341 acatacacag acattagagt ttaagaaagt gtttatggag caagaacttt taaaagaggt
    11401 agtgacttag tttaatttca agaaatgata aatacaaaag aggaaaaaga gaggtgttga
    11461 aatgatagaa aaagtagaag aaaaatctaa gattattaaa acatcaaaag aatataagcg
    11521 aactataatt gaagaaaaag ataaagaatg gaccaaaaaa ttaaatgata tacttagtag
    11581 aagtagtttt tctaatgatg aattagataa aatcaaggcg ttaattccaa aagagatttt
    11641 gactaatgaa aatgttggag aagttgtgac tgaagatgga tatgctaagc ccgagtatga
    11701 tgaggtattt aaatcattgt tcactttaaa taatgaatat gatttacttg taaattttat
    11761 aaatgatatt ctaaaagatg ctccatatgc taacaggaat atcaaaccgt tcactcaaat
    11821 aaagagaata attagagttg aaactgatcc aacaattaat tacattggag agaaaagacc
    11881 taaacttgat attttggctg aagatgaaga aaataatcat ataaatattg agatgcaaag
    11941 agcttttgag aacgattatt tggaaagagc tgaatattat cttagtcgtg tacatggtag
    12001 aaagttagaa gaaggtaaag agtataaaga aattggaaaa actattggaa tacatatact
    12061 aaatcatgta aaatataatc acatagaaga ttatgtaaat tgcctgagat taacaatgga
    12121 cgggcatcca gatatttatt ctagtaaaac tgcattatat tttatagagc ttccaaaaat
    12181 tcataaatct gactatatag taaatagaat tatgctatgg ggaaaactta ttagtaatcc
    12241 ttctaactta gatgtaagag ttatagctaa gaatgattct attattaaaa aagcacttga
    12301 tagattaaaa gaattaggta gcaatgaaga atatttatta aatctgaaaa gaggtgctta
    12361 tatcatgaat aaaagtagaa attttaaaga agagattgaa agagagacag caattagagt
    12421 tgcaaaagaa aaagattata aaataattat aatgttgaaa aaaaatggtt ttaaattaga
    12481 tgacatatta aataaattta atgattttga cttaagtgaa gatgagataa aagaagtata
    12541 tgaaaataat tgatgataaa taaaaagtga acttatataa gtttgctgaa tcggagtctg
    12601 gaaatattta tgtgtatttt ttaatgaatg aaaatttatt ttaaaaacaa taatttagaa
    12661 gtacagttaa ttatattttt atgatactgt acttctagtt ttttctattg tagatttatt
    12721 atttactaat aaatctacaa tagaaatgct tttgttgtaa gaatcatttt atctaatgat
    12781 tgttttttat aatagttaaa tttccatttg tagtgtttaa atataaatct ataacagcat
    12841 ttctataaat gttataaatc actataatga ttcaactcaa agttactgct taaagaagca
    12901 gagattttat caaggtatat catggaaaaa aatattttat tgtaatattt ataagatttt
    12961 attatggaaa taatgtaaaa actttataaa taatatattt tatacctagt tatagataaa
    13021 tttatatagt tattattaaa aaaagcaata aaaagtttaa ttcaatttag caaatttagt
    13081 gatataatta aaaaagaaaa gaaatatgga cttatttgga ataattttga caatctattt
    13141 actacacgtg tagagtcgta tataattaaa ttatacataa aatataaaaa aataatttag
    13201 taatacgtta ggagaaagta ttatgataaa gtcaagctta ttttggaata ttttcattgc
    13261 gatatccttt atgatagaat ggtttctttg taagaaaatg ctagattata caagtgagaa
    13321 aaggatttct aacaaaaaaa taaacttttg tgtggtaggt attattgttt ttgctgacat
    13381 aatatatttt tttgatgtac ctccaaatat aagagtaatt atatctatga taattactat
    13441 tgcattttat aaaatctcat atgatgtggg gtttataaag tctgtattaa tatccttaat
    13501 ttattggatg ttactaatcg ccacagatgc tttgagtata tctttaacag tttggcttaa
    13561 ttctataaat gacatgagta gtatggggac gggagatata tacagaattg agtctataat
    13621 attgggaaag atacttttga ttgtgtcttt gcttttatat agagccttaa agataaaact
    13681 taatatagaa ataaataaaa ggacattatt ttatatagtt attcctatag tcgctaatat
    13741 agcatctttt tttgtaattt ttgaatacat atttaatcca gggtctggta gcttaataca
    13801 taatttccaa ttcgttatga tttctgttgt attgcttata tcaagtatca ctttagtttt
    13861 aattgctaaa aaattacagc aatataatac tttagttgta gaaaataata tgattaaaaa
    13921 tactttgaag tatgaaaatg agtattatga agatatagaa aagaaatatt taaaaactag
    13981 attactaagt catgatatga aaaatcacat cttgtgtata aatgctatga ttaaaaaagg
    14041 tattgatgtt acttcttatt taaataattt gcaggtagaa attcagagta atgacttgtt
    14101 atttaatact ggtaatttta tattagatgc tatattaagt gaaaagaaag aggtttgtga
    14161 taagaatgat atagaactaa aaataggaat aaattttagt tgttgtgaag ctgttgaatt
    14221 agtggatata tgtcatatat tttcaaatat tttagacaat gcaattgagg catgtaataa
    14281 aattgatagt ggaaatagag ttatttgtgt taaaggtgat gttatgcaaa atttttattt
    14341 aataagagtt gaaaatacta agatgaataa gattattact aaaaatggag atattttgac
    14401 agacaaaaaa ggttctcttc atggacttgg aataaaaagt gtaaaaaact ctgtaaataa
    14461 gtatggtggt aatgttgtaa ttgaatatga tgataaaaaa tttgtagtta aaattgctat
    14521 tccacttaga aatgtgaaga aagcagttaa tcatgtgatt taaatgtata taaatttgat
    14581 gtgtagaaat ttaatatata agaattaatg tataaaagtg atgcatagaa gtttaatata
    14641 taagaattaa tgtataaaag tgatgtatag aagtttaata tataagaatt a
//
LOCUS       DBGJFU010000095        14464 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.095, whole genome shotgun sequence.
ACCESSION   DBGJFU010000095 DBGJFU010000000
VERSION     DBGJFU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.095"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(19..447)
                     /locus_tag="ACOIAS_002702"
     CDS             complement(19..447)
                     /locus_tag="ACOIAS_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702404.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
     gene            complement(848..1309)
                     /gene="ribE"
                     /locus_tag="ACOIAS_002703"
     CDS             complement(848..1309)
                     /gene="ribE"
                     /locus_tag="ACOIAS_002703"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGZ2702405.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(1414..2607)
                     /locus_tag="ACOIAS_002704"
     CDS             complement(1414..2607)
                     /locus_tag="ACOIAS_002704"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HGZ2702406.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(2630..3295)
                     /locus_tag="ACOIAS_002705"
     CDS             complement(2630..3295)
                     /locus_tag="ACOIAS_002705"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGZ2702407.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(3329..4441)
                     /gene="ribD"
                     /locus_tag="ACOIAS_002706"
     CDS             complement(3329..4441)
                     /gene="ribD"
                     /locus_tag="ACOIAS_002706"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGZ2702408.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(4573..4694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4883..6679
                     /gene="recQ"
                     /locus_tag="ACOIAS_002707"
     CDS             4883..6679
                     /gene="recQ"
                     /locus_tag="ACOIAS_002707"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HGZ2702409.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKII"
     gene            6778..7365
                     /locus_tag="ACOIAS_002708"
     CDS             6778..7365
                     /locus_tag="ACOIAS_002708"
                     /inference="COORDINATES: protein motif:HMM:NF025846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702410.1"
                     /translation="MREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGG
                     VKFEKYGASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDI
                     QSISQEIEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNL
                     NNIKRKLPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      7454..7565
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7707..9008
                     /gene="thiC"
                     /locus_tag="ACOIAS_002709"
     CDS             7707..9008
                     /gene="thiC"
                     /locus_tag="ACOIAS_002709"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGZ2702411.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9062..9256
                     /gene="thiS"
                     /locus_tag="ACOIAS_002710"
     CDS             9062..9256
                     /gene="thiS"
                     /locus_tag="ACOIAS_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGZ2702412.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            9249..10052
                     /gene="thiF"
                     /locus_tag="ACOIAS_002711"
     CDS             9249..10052
                     /gene="thiF"
                     /locus_tag="ACOIAS_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HGZ2702413.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            10115..10885
                     /locus_tag="ACOIAS_002712"
     CDS             10115..10885
                     /locus_tag="ACOIAS_002712"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGZ2702414.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            10902..12005
                     /gene="thiH"
                     /locus_tag="ACOIAS_002713"
     CDS             10902..12005
                     /gene="thiH"
                     /locus_tag="ACOIAS_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGZ2702415.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12006..12695
                     /locus_tag="ACOIAS_002714"
     CDS             12006..12695
                     /locus_tag="ACOIAS_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGZ2702416.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            12883..14277
                     /locus_tag="ACOIAS_002715"
     CDS             12883..14277
                     /locus_tag="ACOIAS_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGZ2702417.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         5409 a         1751 c         2369 g         4935 t
ORIGIN      
        1 gtttattctc ctaaaatact atataattaa atcctgtaaa ttatcttcaa agctttctag
       61 gtaatcacaa aataatctaa attcttctat ggttttaatt tcttgtaaat ttctcaaatc
      121 tatcattata ttactatatt tatgaaacat aaaattcata tcatacattt cacatttaac
      181 aaattcattt tgataactat atttataagg ttcgtatttt gaaaaactcg actcaaaata
      241 ttttatttta gcataacata atttgtgtat gttgaacaaa agtgcaagaa cagactcctt
      301 aactgcaaac ttatgtgtaa agtattttat cgtgtggtat ttaccatgtt taaactccca
      361 aattaaatcc tcatttaaag taagagaata tttttttata aggttacgcg tatctagtgt
      421 taaattttgt attgaatgta ttttcataat ttattctcct cataaatatt taataagtat
      481 taaattatag tgttttatta ttaattgttc ttaattatat tgtatggtaa attaaaaaag
      541 taataaaata gataattaat atactagtaa tatatgctat aaataaaaac aatagctaaa
      601 atagtaatat atatgttttt atgatatttt atgtttaggg ttatatttgt aattatttta
      661 agcttctaaa agtagatttg atggataacg tataatttta gaaggatatg caatttgttt
      721 gataagaacg tacatatgat taaattaaaa atattaagta ccaaaaagaa gaaagatagt
      781 ttctcccttt ggtacttaat attttgtatc ctttacacta atttttaata tttattaatg
      841 atattattta atctagagac ttaaataaat ttgccatttc tagcgcattc atagcacaat
      901 cataaccttt atttccagct tttgtacctg ctctttcaat agcttgttca atattttctg
      961 tagttacgat tccaaatata acaggtaatt cactgtctaa agatactttg gctatccctt
     1021 tagagacttc actacataca taatcataat gagaagtagc ccctcttata acacagccta
     1081 aacagattat agcatcatac ttaccacttt tagccatttt ttgagcaaca agaggaatct
     1141 caaaagctcc aggtacccaa actatttcta tattttctgg agaaacgtca tgtcttaaaa
     1201 gacagtcttc agctcctgat aaaagttttg atgttataaa ttcattaaaa cgagaattta
     1261 ttatacctat ttttaaatct tttccaataa gttttccttc atatatcatt tttacttcct
     1321 ccaattttat ttatgttata gatttatatt tataaatatc tttaatttaa ttataaaaaa
     1381 ctacttagta gaaaaatatc atcatataat catttatata atatctaaaa gatgtcccat
     1441 tttatctttt ttagttttta aatatagctt attaaagtca ttaatttctt cattaatagg
     1501 gattctattt tcgattttta ttccatattt ctctaattga tttattttat ctgggttatt
     1561 acttaaaaga tttatacttt ttatgtttaa attttttaat atttgagcag ccatatagaa
     1621 atctctcata tcttcttcaa atccaagcat taaatttgct tcaacagtat cgtaaccttc
     1681 ttcttgaagt ttataagcct taattttatt taaaagacca atacctctac cttcttgctt
     1741 catataaatt aaaactccac ttccattttc cacaattttc ttcatagaag attctagctg
     1801 taatccacaa tcacatttta gagagtggaa cacatctcct gttaaacatt cagaatgaag
     1861 tctaactaga gtattttcta tatttatatt tccataagta agggcaatgt gttcttcatt
     1921 tgaataagta tctctatatc ctatgatttc aaaatttcca tattttgtcg gaaggaaagc
     1981 actagatact ttttctatag tagtttcatt tattttgcgg tattctataa ggtcttttat
     2041 agtgatgatt tttaaattat gcttttcttt gaaaatcatt aaatcatcaa ctcttgccat
     2101 tgtaccatca tctttcatta tttcacagat aagaccaatc tctttaaaac ctgcaagtct
     2161 agcaaggtct actgaggctt ctgtatgtcc atccctgaca agaactccac cagtttttgc
     2221 tattaaagga aagatgtgac caggcgtttt gaaatcttta cttgtagaat tttcatcaca
     2281 gaataatttt acagtttcag ctctttcata tgcagatata ccagttagag tatctgctcc
     2341 atctatagta acagtaaagg ctgtttgaaa tgtatctgta ttttctctaa ccataagagg
     2401 tatattaaga cttttaaatt ttttttcagt ggcaggtaga caaattagac ctctaccata
     2461 tgtagccata aaatttattg attcaggagt agccatttca gctgccatca ataaatctcc
     2521 ttcattctct ctactttcat catcaactac gattaccatt ttcccatttt ttatatcttc
     2581 aatagcttct tcaatagtat taaacatgta attcactcct ttattatttt taaaaaccat
     2641 ttttaaataa aaaatcttta tctatatttg atttatggtt actactatta gaatttaatt
     2701 ccttgtagtt attagccata aaattattaa catactttcc aattacatcg ctttctaaat
     2761 taacgaaatc tccaacattc tttgtcaaga gaatagtgtt aattttagta tgaggtataa
     2821 tagaaacctt aaatatttca tcatctaaat atgatatagt taaacttaca ccatcaatag
     2881 caacagaacc tttgagtatc attgaggata aaagtttttt atcaggtcgt atacttacta
     2941 aaactgcatt ttcatctttt tcaaatttgc agatagttcc tttaccatca acatgacctg
     3001 ttactatatg accaccaaat cttgaagaaa gactcatagc tctttcaaga tttaccttgt
     3061 catttgcatt taataaacct aaatttgtgg aacgaattgt ctccatcata acatcggctg
     3121 taaattcatt ttttccgagg tttgaaacag ttagacaaac tccatttacg gctatactat
     3181 ctccaagatt tgtgccatct aatattttat ttgatagtat ggttactttt ccactcttac
     3241 cattagcagt aatttttcta agtattccaa cttcttctac tatgccagta aacatcctaa
     3301 cctcctatat gaatgtatta acaaaaattt aatttaaaac atctgattct ataagaatat
     3361 cgccatctat caaagttaca ttattgattg ttaactgtgt tgaatctgac aaatgattta
     3421 ttccttctcc agaaacgaat gtaggtgcat ctttaccacc tataatcttg ggtgatagaa
     3481 ataatttgac tttatcaact aacttatttc taaagagact gtcattgaga gtagcaccac
     3541 cttcaactag aacagaatca atgtttaact ctcctaattt atacattaca aactccaaat
     3601 ctactaagtt gttttttgaa ggagaaatta ttgtttcaac attattttct tttaacatag
     3661 ataattttat actttctttg caacatgtga aaactatagt tttactggtt ttatctttaa
     3721 ccaaattact attaagtggc aactttaggt gtgtatctat tactattctt gtaggatgag
     3781 acactttttc ttgattgagc ctacaagtta gtaaaggatt atcgttcaaa actgtattta
     3841 tgccaaccat tatactttgg tatttatttc tgtatttatg agttagatac cttgatgatt
     3901 cattagatat ccattttgat tctagtgatt ttgtggcaat tttaccatct agacttactg
     3961 ctgattttac aatacaaaga ggtcttttgt tttttatatg ataaaaaaat gcctcattta
     4021 aatctcttgc ttcatcttct aatattccca cagtaacatc aatattgtta tcttttaatt
     4081 tttttactcc attaccacaa acaagtggat ttgggtctaa tgtagatatt acaactctct
     4141 taatcttatt ttgtataatt ttatctacac aaggaggcgt tttcccataa tgagaacatg
     4201 gttctaagtt tacataaaga gtagagtctt ttagactttg tttagcactg tttatagcat
     4261 ttacttcagc atgattagag ccaaatttct catgatatcc tttgcctatt atattactat
     4321 ctttaacaat tacacatcca actaatggat ttggatttac aaagccttct ccatttttag
     4381 ccaattcaat agcttttttc atataataaa tatctttttc tttttgattc acaaaattca
     4441 caatatgtac acctcctgtt taattggata atttttaaac aaaaaaccct gagaattaac
     4501 tcagggttta gaataatcta caataaatgt ttaataaatg tagaacaaaa ttgctctaca
     4561 aaaacactca ttaaatatct tcttccatcc agactttact gtcggctttg gaatttaacc
     4621 aaatcaacca agttttacat tggctcgcgg gctataccgc cgattgggaa tttcacccta
     4681 ccccgaagat taagtattaa atttatattt agaatgtatc ataaattatt ttactttgca
     4741 aactattttt ttaaaaaata tatgttaata tataatatag aatgtctata aactttgaaa
     4801 taccacgatt gtgaatataa aaataaaaat ttgaacataa tcaggaaaga agtctaatta
     4861 atttaaatta ggagagtaat atatgaataa aaaactttta gaaatactat caaaatacta
     4921 tggatataca agttttagaa aaggtcagga atctataata aattctatct taagcgaaaa
     4981 agatgtgcta gctattatgc caacaggagg gggaaaatca atatgttatc aaattccagc
     5041 tttgatttta gatggaatga caatagtaat ttctccactt atatcactta tgaaagacca
     5101 agtagatgcc ttaaagacta tgggaattag tgctgctttt ataaatagtt cattaagtag
     5161 caaagaattt aatgaaatat taaataatat tagaaaaaat aattataaaa tactctatat
     5221 tgctccagag agattagatg cacaagagtt tattgagcta ataaataata acaatgtaag
     5281 tcaagttgcg atagatgaag cacattgtgt atctcaatgg gggcatgatt ttaggttgag
     5341 ttatagaagg atttctgatt ttataaaaaa tttgcctaaa agacctatag ttacagcatt
     5401 tactgcaaca gcttcagaag aggtaagaac tgatataata aatttactgt gtttagaaaa
     5461 tccagattat tatataactg gatttgatag agagaattta tcaataaata tagtaaaatc
     5521 atctagtaaa aataagtata ttttagatta tattcaaaat cataaaaatg agtctggaat
     5581 tatatatgtt gcaactagaa aggaagttga aaatatatat aatggtttat ctaagagaaa
     5641 tatatctgta tcaaaatatc atgcaggttt atctaaaaat gaaagaagta aaaatcaaga
     5701 agattttata aatgataatg tggatataat ggtagcaaca aatgcatttg gaatgggtat
     5761 tgataaacct aatataagat gggttataca ttacaatatg cctcaaagta tagaaaacta
     5821 ctatcaagaa ataggtagag ctggtaggga tggagaaaaa agtgagtgtg tacttttatt
     5881 ttctccagga gatgtacata tacaaaaata tttaattgat attggagttg aaaatcctga
     5941 aagaaaatta tttcagcata agaagcttca atacatgatt gacttagttt acagtaattc
     6001 atgctataga aagacaatat tgagttattt tggagaaaat tatatagaca attgcaataa
     6061 ttgtagcaat tgtttagtag agggtgaaat agtagataaa actatagatg cacaaaaagt
     6121 catttcatgt gtagctagaa tgaaaagaag ttatggtgtt actactattg tagatgtgct
     6181 tagaggttct aagaataaaa agattttaca gcttggatta aatactttgt ctacatataa
     6241 cattatgaga gattattcta gtgaagactt aaagaatttt gtaaatacat tggtttctca
     6301 tggtttttta gatttagttg agactctagg caatggaaat agaggtagtt ttcctactat
     6361 aagattaaat gagatgtcta tgaaagtttt aaaaggtgaa gtgaaagtag agtttaaaga
     6421 aattgttatg acaaaatcat tggaagtaga ggatgagtta tataatgctt taagagaact
     6481 tagacattca attgcaagtg aagaaaggat agctccatat atggtatttg gagatggtac
     6541 acttagagcc atgtctagta gttatccaat taataaagaa gaaatgctag atatctctgg
     6601 agtaggagaa attaaatacc aaaaatatgg tagagatttt gaagcagtaa ttaaagaata
     6661 tgtcgaaaaa ataatataaa taaaaaaata gatgaaaaaa atgtcgattc aaagaacgtt
     6721 aatagtgatt tcttagaggt taatacagat aaagttttat atgaaaaact gttagtcatt
     6781 agagaagaat atgcaacaaa ggaaaaatta cctgcatata tgattttagc caataaggct
     6841 ttgaaagaaa taagtggaag atatccactt gatgaagaac aattaaaaga tatttctggc
     6901 ataggtggag taaaatttga aaaatatgga gctagtattt tggcagaggt taataaatat
     6961 gtaaaagaaa ataatattaa tgttatttgg gagaaaaagg gcaaaaggaa gttaatatta
     7021 gatggagaaa gtagaaaaaa taatgaaata gcattagatt tgctaaatca aggaaaagat
     7081 atacaaagta tcagtcaaga aatagaagtt tcactgtcaa ccattattgg atatgtgcat
     7141 gactatgtaa aggatggaca tagtatatca tttgaattag gactagaaaa tttttataat
     7201 gaagataaaa agaagatgat tttagaagtt tgcgaaaaag ttggctatga taatttaaat
     7261 aacataaaaa gaaaacttcc agattcaata aagtatgaaa atataagagc tgtgatactt
     7321 gactcttatc ttgaaaactt aaaaaataat attatagcag agtaattttt aacttaataa
     7381 aaaataaaat ttgtattatt gtgttgaact aataattttg ctgtgttata ctgaatttgt
     7441 aaaaataatg aaaaattaga gctaggggcg ctagaattgg ctagctgaga gataaagccg
     7501 taacttacaa accctaataa cctgatcaag ataatgcttg cgaagggaag ctttttaata
     7561 taattttatt atataaatag gcaaattatt caatgttagg agaaatttgt ctaagtgtat
     7621 atacgattat aagagtgctt tccaaacgag gaaaagcact ttttttattt tcaaaaacta
     7681 taactttaat cttggaggat tgtaatatga actatacaac acaaatggat gctgctagaa
     7741 aaggcataat aactaaagaa atggaaatcg tttctcaaaa agaacaggta gatgtaaatg
     7801 aattaagaga attaatagca aatggacaag ttgttatacc agctaataaa aatcataaat
     7861 ctttaagtgc agaaggtgta ggtaaaaatt taagaacaaa aataaatgta aatctaggaa
     7921 tttcaagaga ttgtaaggat atagaaaaag aacttgaaaa agttagagta gcaatagaca
     7981 tgaaagctga agctataatg gatttaagta attatggcaa aactagagaa tttagagaaa
     8041 aggttgttga aatgtcacct gcaatgatag gttcagtacc tatgtatgat gcagtaggat
     8101 atttggaaaa agaattaaaa gatataacag aagaagagtt tttaaatgta ataagacaac
     8161 atgctattga tggagtagac tttattacta tacatgcagg tcttacaaga agtgtttgcc
     8221 aaaaaataaa aaatcatgag agattgactc atatagtatc tcgtggaggt tctttattgt
     8281 ttgcatggat ggaattaaat aataaggaaa atcctattta tactaatttt gataaaatat
     8341 tggatatatg tgaagaatat gatgttacat tgagtttagg agatgcttgt agaccaggtt
     8401 gtataaaaga ttctactgat ggtgttcaaa ttcaagaact tgtagtatta ggtgaactta
     8461 ctaaaagagc atgggaaaga aatgtacaag taatgataga aggtcctgga cacatggcta
     8521 ttgatgaaat agaagcaaat gtagtattag aaaagagatt atgccatgga gcaccttttt
     8581 atgttcttgg accattggtt acagatatag caccaggtta tgaccatata acaagtgcaa
     8641 taggaggagc tttagcatgt gctaaaggag tagatttttt atgttatgta actccagcag
     8701 aacatttaag attgcctaat ttagatgata tgaaagaggg tataatagct gcaaagatag
     8761 ctgctcatgc aggagatata gctaaaaatg ttaaaggtgc tcgtgaatgg gataataaga
     8821 tgagtaaagc tagagctgat ttagactggt gtgagatgtt tagacttgca atagaccctg
     8881 aaaaagctaa gagatacaga gatgaatcaa ctcctactca tgaggatagt tgtactatgt
     8941 gtggaaaaat gtgctcaatg agaacagtta agaaaatatt aaacaatgag gaacttaatc
     9001 taatataatt tttggctagt atgactggaa taaaaataag ctgaaaagag gaggtgattt
     9061 agtgaaggtt aatgggaagg aaattgaatt tgaaaaagat ttgactgtta tagatttatt
     9121 aaataaatat aatttaaaaa gtgatagagt tgtagttgaa gtgaatttag agataattga
     9181 agaatctaac tacaatacat atgtattaaa agacgaggac attgtagagc ttattagctt
     9241 tattggaggt ggttaaaata aaaatatttg taaatgaatt attaacagat gttgaggaaa
     9301 atacaactgc ttataaagtt agagatagct ataacaataa atgtgatgta ttggtgttaa
     9361 atggatatcc aattaaaaca gatatgaccc tttgtgaaaa tgataaatta acacttatac
     9421 aaaaaggagt taagccatca cttgatgaat tggaaagctt gttgattgct agacatactc
     9481 caaatataca taataaatta aaaaaaggga aagttgctat attgggattg gggggacttg
     9541 gttccaatat agctatctct ttagctagaa taggagtggg agaactttta cttatagatt
     9601 atgatgttgt agaaccatct aatttaaata gacagcagta ttttatagat gatataggaa
     9661 aactaaaaac tgatgccatg atagaaaata taaaaaaaat aaatccattt ataaaattaa
     9721 ataaacgaga tattttttta aacaagaaca atatggatac tattaaggat tcagatttaa
     9781 taattgaggc ctttgatgat gcttcatgta aagcacaggt atgtaattat gtattaataa
     9841 atttaaaaga taaatattta atagcatcat ctggtatggc aggttactat gattcaaata
     9901 taataactac taagaagata aaagacaggt tttatatatg tggggatttt gtgaatgaag
     9961 ctaaatttgg tgaagggctt atggctccaa gggttgccat ctgcgcaaat catatggcaa
    10021 atttggcaac gcaaatactt atagatatgt aaatattaaa attagatata aaaaagattg
    10081 aatattaaaa aaatttaacg tggagggaaa taatatggat aaattggtac ttggtggaca
    10141 tgaatttaat agcagattat tggttggaac aggtaaatat ggttcaaata atatattgcc
    10201 tgaggtaata aaagaaagtg gaagtgaaat aataacaatg gcacttagaa gagtggattt
    10261 agacaataaa caagaaaaca tattaacgta tattccaaaa gagatgacaa tacttccaaa
    10321 tacatcagga gctactaatg cagaagaagc tgtaagaata gctagaatat ctagaaaaat
    10381 gggatgtgga gattttataa aaatagaagt aatatcagat actagatatt tacttcctga
    10441 taatgaagaa actataaaag ctacaaagat acttgctgat gaggggttta tagttcttcc
    10501 atatatgaca ccagaccttt atgctggaag aagacttata gaagctaatg cagcggcagt
    10561 tatgccactt ggagctccaa ttggttcaaa tagaggtctt caaatgaaag aaatgataag
    10621 aataatgata gatgagttgg atataccaat aatagtagat gcaggtattg gaaaaccatc
    10681 tcaagctatg gaggctatgg aaatgggagc tgatgcagta cttgtcaaca ctgctatagc
    10741 ttcagcagga gaccctgtac aaatggctag agcatttaaa ttagctgtag aaggtggaag
    10801 agaagcatat attgcaaaaa caggaaatgt gtcagaattt gcaaatgcat cttctccttt
    10861 aacaggtttt ttaggcaatt tatagaaagt ggtgattggt gatgagtttt tatgatgtaa
    10921 tagaaaaata tagggatttt gattttgatg gatatttaaa taatgttact gataatgatg
    10981 ttttaagaag tttatcaaaa gacaagcttg aagattttga tatattaaat ttgctttcta
    11041 aaactgcagt aaaacattta gaagatatgg cacaaaaagc acataaatta agtgttcaat
    11101 actttggtaa aacagtatgt ttatatacac caatgtatat agcaaattat tgtgttaatc
    11161 aatgtgttta ttgtagctat aatataaaga gtggaataaa aagaaaaaaa ttaactatgg
    11221 atgaaattag agaagaggga gaagcaattt caaaagaagg tttcaaacat ctacttgttt
    11281 tgactggaga aagtagtttt cactcaagtg tagaatatat aggtgaggct atagaaatat
    11341 taagagagaa atttccctct ataggtatag aagtttatcc aatggaagta gaagaatata
    11401 agtacattgt agataaaggt gttgaaggat taacagtata tcaagaaact tatgacgagg
    11461 aaatatataa gagagtacat ataaaaggcc caaaatcaaa ttataaattt agactagatg
    11521 ctcctgaaag aggggcaaaa gcaggtatga ggactttatc aataggtgca cttttagggc
    11581 taaatgattt tagaaaagaa acttttttta caatattgca tgggaaatac ttaaaaacga
    11641 aatatcctca tattgagctt tcatattcaa caccaagaat gagacctttt aaagggtgtt
    11701 ttgaggaatt ggtagatata agtgatactg atttagttca agctatggta tgtatgagat
    11761 tatttgaccc tcatgcagct ataaatatat ccactagaga aaatttagaa atgagaagtc
    11821 atataatacc tttaggagta actaaattga gtgcaggtgt atctactgat gtaggaggtc
    11881 attctcaaga tgaacatgat actgctcaat ttaagataaa tgatgaaagt actgttaaag
    11941 atgtggagaa aatgctaaac tcaattggtt atcagcatgt gtttaaagat tgggaaagat
    12001 tttaaatgta tctgataaca aacagaaaat tatgtagtga ggaacgatat ttagaagtta
    12061 ttaaagagtc tatattgagt ggtgtagaaa atataataat aagagaaaag gatttagaat
    12121 atcaagagct aagaaaatta tatatgaaaa taaaaactaa aatcaattgt atagattttc
    12181 aagagcaaat atcagatgaa agtttgaaaa ctaatataaa tcaaaaagag tgtagaaata
    12241 aattcaaggt taattttata ataaatagta acattgaatt ttttgaaaaa gtggattgtc
    12301 aaggtatcca tcttcctttt aaattattct tgaatttaat tgaaaataag tataatttta
    12361 atgaaaataa aatactagga ttatctttac ataaagtaga ggaagtagat tatttagaaa
    12421 aattaataag aaatcaaaat ataaaaatag attacataac attatcacat atatatgaaa
    12481 ctaaatgtaa agaaggatta aatccaaaag gaattgagct tttaaaagaa gctaaaaaga
    12541 ttactgatat taaaataatt gcacttgggg gaattttacc atctaatgtc aaggaaacat
    12601 taaaatactg tgatgatttt gctataatgt caactataat gaggtctaaa gatattaaaa
    12661 aaaccatatc aaattataat gaaaagttga attagtaaat atagtatatt gaatttttaa
    12721 cttaaataaa aataaaaaag ttatctttta aattttaaaa ggtagctttt ttattttata
    12781 tctttgctaa taaaaaattg taaaaaggga aaactttagt cagagagatt aaacaatatc
    12841 ctagaaagat ttaaaattta gtaaagtatg gaggacatta taatgactga cagtaaaaag
    12901 aaacgattta ttataccaca cacttttaca ataatatttt tactaatagt cttgatggca
    12961 atattgactt ggatagtacc aagtggtgaa tttagtagag aagatgtaga aggtagaatg
    13021 gtagttgtac cagggactta tcaaagtgta gaaagtaatc ctcaaggatt tgcagatgtt
    13081 tttaaagcac caatagaagg ctttatcgat tcagctgaag ttgtaggttt tgttttaata
    13141 gttggaggtg cttttggtat tgtaaatagg actggagcta tagaagcagg aatagcttcg
    13201 gttatctcta agtttaaggg aaaagaactt cttataattc caatatcaat gattttattt
    13261 ggacttggag gtacaacatt tgggatggca gaagagacat taccatttta tatgatattt
    13321 gttccactca tgacatcctt gggatatgat tctctaacag caatagcaat tgtatttata
    13381 ggagcagcag caggagccgc cgcttcaaca ataaatccat tttcagttgg tattgcacag
    13441 gcactagctc aacttgctcc tggttcagga gtaacttaca gagtagttat ttttatagca
    13501 tatatactaa taagtatagc atttgttatg tactatgcta agaaggtaaa ggaaaatcct
    13561 gaaagttcaa tagtatatga aatagataaa aataataaat caatacttaa tcaaacttca
    13621 acagaaataa aagaatttac ttttaaagaa gtatctgttc ttgctatatt tggaataggg
    13681 atggcaataa tgatttgggg tgttttaagt caaggatggt acattccaga aatatcaatg
    13741 tgttttgctg caataggtgt gctttctgga ttggttggta gattatctca aattgaaatc
    13801 tcagaaagtt ttatagatgg agcaaaagat ttagcatctg cagctcttgc tatagctttg
    13861 gctcgtggaa tcgtaataat tgctcaaaat ggatttataa tagatactat attaaattcc
    13921 gcagcagcct ttctaggtgg gcttccaaag gttatatttg tatatttatc atttttcttg
    13981 gaagctgcat tggcattttt aattccatca tcttcaggtc ttgcttcact tacagtacct
    14041 gtacttgctc ctcttggtga tttggttaat atatcggctc aagtgatagt aaccgcttat
    14101 caatttggtg tgggtcttac aaactttata actccaactt caggagtact gatgggagca
    14161 cttgcagtag cccatattcc attttcaaaa tgggtaagat ttataatgcc tatcttgttg
    14221 attttgacag ctgtatctct agtcttttta acaataggta tttatatagg gttttaatta
    14281 agtgtagttt tatttaagat atttagtaat ggtataaaat gataatatgt tttcaataaa
    14341 ttgccactaa tttaattagt ggtaatttgt tttttgtatt taaaaataaa tatatttact
    14401 tgtattttga attaatatct acttagacat tgttttaatt gttatatttt catttaatta
    14461 agca
//
LOCUS       DBGJFU010000096        14125 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.096, whole genome shotgun sequence.
ACCESSION   DBGJFU010000096 DBGJFU010000000
VERSION     DBGJFU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.096"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            428..1321
                     /locus_tag="ACOIAS_002716"
     CDS             428..1321
                     /locus_tag="ACOIAS_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HGZ2702418.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1321..2715
                     /gene="gltA"
                     /locus_tag="ACOIAS_002717"
     CDS             1321..2715
                     /gene="gltA"
                     /locus_tag="ACOIAS_002717"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HGZ2702419.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3081..5300
                     /locus_tag="ACOIAS_002718"
     CDS             3081..5300
                     /locus_tag="ACOIAS_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2702420.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5447..6193
                     /locus_tag="ACOIAS_002719"
     CDS             5447..6193
                     /locus_tag="ACOIAS_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702421.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6193..8073
                     /locus_tag="ACOIAS_002720"
     CDS             6193..8073
                     /locus_tag="ACOIAS_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702422.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            8192..9583
                     /locus_tag="ACOIAS_002721"
     CDS             8192..9583
                     /locus_tag="ACOIAS_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2702423.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9690..10730
                     /locus_tag="ACOIAS_002722"
     CDS             9690..10730
                     /locus_tag="ACOIAS_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGZ2702424.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11219..11872
                     /locus_tag="ACOIAS_002723"
     CDS             11219..11872
                     /locus_tag="ACOIAS_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGZ2702425.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            11948..12157
                     /locus_tag="ACOIAS_002724"
     CDS             11948..12157
                     /locus_tag="ACOIAS_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702426.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12297..12863)
                     /locus_tag="ACOIAS_002725"
     CDS             complement(12297..12863)
                     /locus_tag="ACOIAS_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HGZ2702427.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13024..13677
                     /locus_tag="ACOIAS_002726"
     CDS             13024..13677
                     /locus_tag="ACOIAS_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGZ2702428.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13835..14011)
                     /locus_tag="ACOIAS_002727"
     CDS             complement(13835..14011)
                     /locus_tag="ACOIAS_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702429.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5529 a         1400 c         2375 g         4821 t
ORIGIN      
        1 atgaaagctg gtattgcaat agattaaagc cttatacacg tttagttttt aaagaaatta
       61 gtgttattat gaaaacctaa gttaatttat taaataaata attgaataaa aaaattttat
      121 tatgtattta taaaattttt gttacaaatt tggcataata tagtatactt taagtgtgta
      181 ttaggttaaa tctataacaa attagacgta tgatatagac ttagtgttta ttggcattta
      241 caagaaattt gttaatatca actatttgta gtttgggcag ttataattat tatagggggg
      301 atgtccaata caaaacaatc aaccaagaac taatattata ttataaagag ttatagtttt
      361 gtgtgtttat gtttttaatt aaatttaaag tgggaaaaat atatttgtta gaatggagaa
      421 tagcgagatg agtaataaaa tagttagtaa gagacaatta acagatagta tttatttaat
      481 ggaaatagag gcacctagag ttgcaaaatc ttctcaacca ggtcaattta taataattaa
      541 aaatgatgaa aaaggagaga ggattcctct tacaatagct gactatgata gagaaaaagg
      601 aacagtaact attgtattcc aaacagttgg tgcttcaact aaaaagttag caatgtttga
      661 agaaaacgat tttgtaatgg attttgtagg gccattagga caagcaagtg aatttataca
      721 tgaagatata gaagaattaa gaaataaaaa gatattattt gtagctggtg gagttggttc
      781 tgctccagtt tatccacaag ttaaatggtt taaagagcat ggattagatg tagatgtaat
      841 aattggtgca agaactaaag aattaattat attagaagat gatatgaaaa aagtagctaa
      901 aaatgtatat gtatcaacag atgatggtac atatggattt aatggaagag ttacagacct
      961 attaaaagat ttagttgata atcaaggtaa aaaatacgac caagcaatag taattggacc
     1021 aatgataatg atgaaattta tgtgccaatt aactaaagaa ctaaatatac ctactatagt
     1081 aagtttaaat acaataatga tagatggaac aggtatgtgt ggtggttgta gagttagtgt
     1141 tggaaatgaa actaagtttg catgtgtaga tggtcctgaa tttgatggtc atttagttga
     1201 ttttgatcaa gcaatgagaa gacagtctat gtacaagact caagagggaa gagctatgct
     1261 aaaacttgaa gaaggcgata gtcatcatca tagtaattgt ggatgtggag gtaacaaata
     1321 atggatgcta aaaaagtaaa agtaccagtt agagaacaag aacctgctgt gagagcaaca
     1381 aattttgatg aagtatgctt agggtataat aaagaagaag ctatggcaga agctaataga
     1441 tgtttggctt gtaaaaaacc aaaatgtgtg ggtggatgtc cagtaggaat cgacatacca
     1501 gggtttataa caaaaattaa agaagatgat atagaaggtg ctgctaaagt aatagctaag
     1561 agcagttcat taccagctgt ttgtggtaga gtatgcccac aagaaagtca atgtgaaggt
     1621 gtatgtatac ttggaataaa atcagatgct gtatcaatag gtaaattaga aagatttgta
     1681 gcagattggt caaaagaaaa tgacataaat ttatctgata cagagcctaa gaaaaatcaa
     1741 aaagtagcag taataggaag tggtccagca ggtctagctt gtgctggtga tttagctaaa
     1801 aaaggctatg atgtaacaat ctttgaagca atgcatgagc caggtggagt tttaacttac
     1861 ggaatacctg agtttagact tccaaaacaa gctgttgttc aacctgaaat agacaatata
     1921 agaaaattag gtgtaaaaat agaaactaat gtaattgttg gtaaaacaat tacagtagat
     1981 gaacttatag aagatgaagg atttgaagct atattcatag gttcaggagc tggtcttcca
     2041 atgtttatga atataccagg ggaaaatgca aatggagtat tttctgcaaa tgagttttta
     2101 actagagtaa acttaatgaa ggcttataga gatgattatg atacacctat tagttctggt
     2161 aaaaaagttg ctgtagtagg tggtggaaat gttgctatgg acgctgctag aactgcatta
     2221 agattaggtt cagaatcata tatagtttat agaagaagtg aaaaagagct tccagcaaga
     2281 gctgaagaag ttcatcatgc aaaagaagaa ggcattatat ttaatacatt aacaaatcct
     2341 aaggaaattt tagtagatga aaatggatat gttaaaggta tggtttgtat aagaatggaa
     2401 ttaggagagc cagatgattc tggaagaaga agacctatag aaatagaagg ttctgaattt
     2461 gtacttgatg tagacacagt tataatgtca cttggaacaa gcccaaatcc actaatatct
     2521 tctacaacaa agagtcttga tataaataag aagagatgct taataacaga tgaaaatggt
     2581 cagacttcta aagagggtgt atttgcaggt ggagatgctg ttactggagc agcaacagta
     2641 atatctgcaa tgggtgctgg taaaacagca gcagcatcaa tagatgaata tttaaaagca
     2701 aaagttaatg cttaatttgt aaattaaaga gagggtgtct caaaatgaac tttttagttc
     2761 atgaggcact ctttttgtac aaaatcaaat atgttttaat catttcaaca atgaaaattt
     2821 cattcctgta gaaagtctaa cttttaggtc ttagtacaat ctctattttg atgtagccac
     2881 ttttttagat ggtttaataa tttatcagtt attattgtaa tatttgatac taagtaacat
     2941 aactttgtct aactatgata aaatagttat acaataaagg tcttgtaagg agtgaataat
     3001 gtgtggctaa taaaatcaga tttgtcaaat ctaacattaa ataatagaaa aataaattgt
     3061 gtaattaatg gtattttaat atgaaaaaaa gagaaaaagt atttgatggg ctagttttta
     3121 agcatatatt aatcttatta atactattag ttttgattga tttaacaagt accatcatat
     3181 atgcaagtaa ctctatggaa attacttcta gaaatatgat tgaggcatca aaaagagaac
     3241 ttgaaaacta tttagaagta aacatatctt tattaaaagc tcttagtcaa gatgatagat
     3301 tttctaatgg tgaaacatca ctaattgaaa agggaaaatt attaagtcca tatcaaaagg
     3361 aatataatct ttttatgatt ggtataacag atacaaaagg aaatgcttca agtacatata
     3421 gggaaaatgt taactcaata aaagataagc ctagttttga gaaagcaatt aaaacaaagc
     3481 aagtagtagt ttcagatatt gaagtcagta atgtaacagg tgacaaagta tttatcatat
     3541 atgtaccaat tgtaaaaaat aatgagatga ttgggaccat ttttgcttct ttttattttc
     3601 aagatgtaaa taatttaata agtagaagca attttgatga ttctattaaa tttctaatga
     3661 tagataaaga ttatactatt atttctcatc caaacaaaaa atatgtaaat gataaaagta
     3721 agatgcttga tttagaaggg aatattatag gaaccactaa aagtgaaatc ttaaaaaata
     3781 taaataaaaa atgtcaaggt gattttttat cttgggataa ttggagatta tataatgtaa
     3841 aatatacgaa cataaagtgg actaattgga ctttagtatc aaaatgtaat atatttaaga
     3901 actttaaaaa tctaatagtt aattttatga taaagttgta tttttatata gtaatattta
     3961 tgatattgtg gaaattaagt aatgctaaat taatagaaca acttaaaaaa ttggcttatt
     4021 atgattcttt aagtggaata aaaaataaag aaaagtttag aaaagattct atgtatattt
     4081 taaagaatta ttatcaagat aatttttact tagtacaatt ggatgtcaat aaatttaaat
     4141 atataaatga gatgtttgga tatgctgaag ggaataagat attaattcat atatcacagg
     4201 ttttaaataa taatacaaat aaatatgaaa tttgtgctag gatggataat gaccatttta
     4261 tattgttgat tgcttgtaac actgaagatg aacttttaaa tagattatct aaaataaata
     4321 aagaaatttg taacttaaat actactaact catctaagta taaaattgtt atgtcaagtg
     4381 gaatatataa gattactaaa aaggatgata ttaaaaagat tgacttgtta attgatagag
     4441 caaatattgc agcaaaaagt aaaaaggaaa aatatgaaca ttcatattca ttttttaatg
     4501 aagatacaag aaatcgattg tacaaggaaa aacgattgga agataatatg aataaagctt
     4561 tagaaaaagg tgaatttata gtatattatc aacctaagta tagtttagat gatgtaaatg
     4621 aaattgaagg agcagaagcc ttgataagat ggaatagtcc agaatttggg tttatttcac
     4681 ctatagattt tgtaccttta tttgaaaaga atggttttat tgtaaatatt gatatgttcg
     4741 tgtttgaaga agtatgcaaa acattaaaca agtggataaa taaagggtat actcctgtac
     4801 caatctctgt aaatatgtct agagttcatt tatataggga taattttata gaaaatataa
     4861 ctgatttgat aagtaaatac aatatatctc ctgagtttat tgagcttgaa ctaacagaaa
     4921 gtgttgtgtt tgataattta aatatattga ttgatataat gaaaaagatt aaagagatag
     4981 gattccttat atcaatggat gattttggtt cgggatattc atctttaaat ctgttaaaag
     5041 acctttcttt tgatatttta aaattagata gaggattttt aatcgaaaca acagatacaa
     5101 aacgtggaaa aattattatt tctaagattg tggaaatggc aaaagcgatt gatattaaag
     5161 taatctgtga gggcgtagaa acttatgaac aagtagagtt cttgaaagag ataggttgtg
     5221 ataaggttca aggatactta tttgctaaac ctatggtatt agatgaattt gaaaaacact
     5281 taaactttaa atttgattaa tcattaataa ttattagact ataagatacc ctaacaactt
     5341 acttacaaat atgtaagtta attgttaggg tatttttatg gttaaagtag tatattcacg
     5401 atattatggt attgtagtaa aaataaaaaa ttatggagaa tataaaatga acatattgag
     5461 tattagaaat atatcaaaaa catattgtgg aaatattcca tttaaagcat tagataaggt
     5521 tagtctaaat atagaaaagg gagaatttgt gtctgttatg ggaccatctg gaagtggtaa
     5581 atctactctt ttaaatataa tatctacagt tgatagacag agtgaaggtg aggttgtatt
     5641 agatggatat gatgtaagta aattgaaagg agaaaaactt gctgaattta gaaggaaaca
     5701 acttggtttt gtatttcagg attttaattt gattgacact ttaactgttg gcgaaaatat
     5761 tatgttgcca cttacactag agggggcaag tatcaaagat atgaatatac aaactaaaag
     5821 tatatctaaa tttttaggaa tagataaaat tttagataga aaaacctatg aaatttcagg
     5881 aggtcaagct caaaggtgtg ctattgctag agctattata aataaacctg ccatacttct
     5941 tgcagatgag ccaactggaa atcttgattc aaaatctact gatgatgttt tgaaattatt
     6001 tactaggata aacaaagaac aaaaggtaac aacacttatg gtaactcatg aagcatatag
     6061 tgcaagttat tcagatagag taatttttat aaaagatggt tgtatatata cagaaataaa
     6121 aaaatctgaa agcagtaatt ctttctattc tgatatacta gctgttttgt ctcaaatagg
     6181 aggtgtaaga tagtggattt tagaaagttt gcttataata atatagttag aaatttcagg
     6241 gcatatttag catatttttt aagttctgtt ttttctataa tgatattttt tgtttttgca
     6301 gtgtctatgt tccatccaat tataacaaaa tctggaatgc agagtggttc tactgcattt
     6361 atggctctta tgacttcaga attaataata ttcggtttta gtctactttt tatattgtac
     6421 tctttaggaa atttcttaaa atatagattg aaagattttg gagtattgat gataattgga
     6481 atgagtaaca gacaactgaa aaaattgata ttaattgaaa atttgataat aggattttta
     6541 gcagtgatgt taggaatatt acttggttta tcaatttcaa aattgttttt attgtattta
     6601 agcaaactgt tttatatgaa tttaactgaa ttttattttc cagtaaaagc aatgctattg
     6661 actattgtat catttatgat tttatttttg ataacaggac caatgagctt aaaattattg
     6721 agtaaaaaga gcattttaga gcttttagta ggtacaaaaa agcctaaaaa agagataaaa
     6781 tcttcaaagc tatttggaat aattggtgta atatgtctta tattaggata tgctatgata
     6841 ctatttggca ataagttaaa aataaatgga ggtatgtata caacagcact attaattact
     6901 ttggggatat ttttattttt ttctcaattt actattttga tattaaagta tttaaaaaat
     6961 aaagaaaaat tttataagaa caaagtaaat atgttactta taagtaattt gatatataaa
     7021 ttaaaggata atacacgatt gttattttta ataacaatac ttttatcggc tactttagta
     7081 gcatttacaa caacttcaac acttgtaagt tctcaaggag aagcttctaa aaataatttc
     7141 ccaatggttt actcatattt ttcagagcat aataatgaaa aagaatatga agaactacaa
     7201 caaattagag aagcaatcaa aggatatgat tataaggagt taagttttcc tgtacttaaa
     7261 tataataggg aagtcttact aagtgtaaag aattataaca aattatcaaa ggaattggga
     7321 ttagataaaa ttagttcaaa gttagaaaaa tatgaaggga ttataattcc tagtagcaat
     7381 tcgcaagaac atataaattc tttaaaacaa ttaaaaaatt atagtataaa aaatttaaat
     7441 atagaagtaa aaaaatcaaa aaataaaata atttttccta caggactgtt tagtaaagtt
     7501 gtggttataa gtgatgactt atacaatgaa gtaaaaggtg aattttcaaa aattaatttt
     7561 catggatttg attataaaaa ctggcaaagc agtgcaaaga taactgaaaa tttaaaagaa
     7621 aaacatttta atttggatag agaatataac aggtcatatt ttttaacatt accagatatg
     7681 tatttagtag agttacaaca aagtaaaatt cttcaattta tgggtgtttt tataggagtg
     7741 atattattta tagcagtttt tagtttttta tacttcagat tatatacaga cgagatagaa
     7801 gataatgtaa agtacaaaaa actatcaaaa ataggtctat cttttaaaaa tatgaataag
     7861 attgttagta tagagatagg tacattgttt tttataccgt attttatagc aattataaat
     7921 agcataattt ctttactgtt tttaaatcaa gtattaaata atagttttaa gttagaaaat
     7981 ttgggtatat tactactgat atcatctata tattcacttt atttttactt attaaaaaaa
     8041 aattatacta gaaaagtttg gttttttgat taaagtggta tataatagat atataaatta
     8101 atatttaaaa agtagttaat attaggggag ggcatttgca cctcccttgt aatatgtgga
     8161 aaattgaata ttagaagaag gaggagactt gatggaaaat caacaattat taggaacaga
     8221 acgaattagt aaattactac taaaatattc tataccagct ataataggta tgttggtaaa
     8281 tagtttatat aatgtagtag atagaatttt tattggaaat ataccagggg ttggaccact
     8341 tgcaataaca ggtcttggtg taacaatgcc aataatgaca ataatacttg catttgggat
     8401 gttgataggt ataggaacta ctactaccat atcaataaaa cttgggcaag gtaaagtaga
     8461 agaggcaaga aaacttatag gaaatgcaat gacgttatct gtaataacag gaattatcat
     8521 aatgatactt ggaatattat ttgcaaataa aatacttaca ttgtttggag caagtgaaaa
     8581 tactttaata tatgcaaaat catatatcaa tattatatta ttaggtacag tagtaaatct
     8641 tctttccttt tctttaaacc actcaataag agctgatggt agtcctaaaa tttcagcagg
     8701 aataatgata gtaggatgtt taactaatat agttttagac tggatattaa tatttggatt
     8761 caatttggga atacaaggag ctgcaattgc tacagtaact tctcaggctc ttacagctat
     8821 tttaactata gggtattata taagtggaaa atcaaactta cgattcagta aatctaatct
     8881 aaaattagat aaaaagctaa taaaagcagt gtttgcaata ggtatgtcgc catttgctat
     8941 gcaacttgca gcaagtttag ttcaagtaat atcaaatatt gcacttaaaa ctcatggtgg
     9001 agatttagct ataggggcta tggctactat ttcttctatt gcaatggtat ttttgatgcc
     9061 tatctttgga ataaatcaag gagcgcaacc tattataggt ttcaattatg gagctgaaaa
     9121 atatgatagg gttaaaaaag catatttagg ttcattagta gtagctacta taattttatg
     9181 tatgggtatg gtagtagtta tgcttttccc agaagctatt ataggtatat ttaataaaga
     9241 ccctgaactt atgaatatat ctgttaatgg attaagaata tacttactta tgttgccaat
     9301 tgtaggctta tcagttacag gaacaaactt tatacaatct ataggaaaag caaaaatggc
     9361 tatgttattg agccttttaa gacaagtaat tttattgata ccagcagtat taattcttcc
     9421 tacattctta ggtttacagg gtgtatggac agctcaacca gtgtctgatt ttatagctac
     9481 agtaattaca ggtatagttg tatttagaga attaaaaaga tatactccta aaactgaaaa
     9541 attaaatgaa aatgaaagac taaacgagat aactactgag taaaaatata aattcagata
     9601 attgagcaag aaattgcaaa aaatacgatt atatgataaa ataaaaccgc tgtcaaggat
     9661 aagagaaatt ctaattatgg agtgatttta tggaatttac aaagaaaagc aaattaaaaa
     9721 atatgtatgt aaacatgaat gtagctagaa tgttgtctaa gataaatgag tataagggta
     9781 gacaactgct ttacaaaaaa caaccaaaag aaattttaga aaacttagaa aaaaaatctt
     9841 tagtagattg ttctgaatct acatacattg gaaatcaaag agatagtagt aattttaatc
     9901 tagaaaagtt gataagtaat gaagttacac ctagaagtag agaggaattg agtattgttg
     9961 aatacagaga tgtggtaaaa actattaaca gtgcttatga gagtattccc atcagttcac
    10021 aaacaattct tgagcttcat ggatacttat ataaattttc atctactaga ggaggaagtt
    10081 ataagtcaga taatgatttt attgaacaca atttaaaacc tagcgaattt ataagtactg
    10141 attcaagtgt caataaagtt gaaaaagctg tagaagaaat atgtgaagct tataatacat
    10201 tgatagagga agatgaaatt gatatattaa tattaatttc tgcatttgtg ttagatttca
    10261 tattgataca tccattcaaa gaaggtaata taaaaatggc gagagtactt atcctattgt
    10321 tactaaataa aaatgggtat gaagttggaa gatatataag tttaggaaag atatttgatg
    10381 atagttcata tgaatattat agtaatttaa attatttgaa agcctcaata ggtagtgaaa
    10441 aagcagatat gaatgcatgg attgagtatt ttttagagac gatattaact gcatatgaga
    10501 agctggatga tagtttaaat atatctgata agaaaagaca aacaaaaact agtagaatag
    10561 agaaaataat aaattctacc cttggatatt ttacaaaaga agatataaga gatttatgtc
    10621 ctgacattcc agaacctact ataaatagag tttttaataa tctaagaaaa caagataaga
    10681 tagaagttgt tgcaagagga agaagtgcaa aatggaagaa aaaatattaa aaaatgttta
    10741 gataactaaa atggtatagt gttttacact ataccatttt taatgtcatt agcatgagca
    10801 ataatttaac aacttaagca atatcaattt tatttattga ttatttttga actgaaaaat
    10861 ggcgttatat ggtatacctc agtggtaatt aattttggat gagaagatat aatattacag
    10921 aatatattag gagtatatgt agaaaaaata taataaaagt gtgaggctta gtaattaaca
    10981 aattatgtgg cttcacactt ttttataaaa taaagatact tagcagaata atattttagt
    11041 tgatatgttt ataagatggt acttgtttgg gtaaaagtat aaatagtaaa aaagtagaag
    11101 taatgtgtat aaaaacataa gttacattct gtaataatag ttattttata attcaacaaa
    11161 agaatagaat gggtatattt gtgaaataat aatataaaat aatcttagga gtgagcttat
    11221 gaagaaaaaa ttattatata taaatgtaaa ttcaaaacct gaagacttat cttcaagtaa
    11281 aactgtagca agaaaattta taaataaatt tatggaaaaa aataaagatt ttgaggttga
    11341 agagatagac ctttataaag aacatatacc aagacttgag tatcagtact ttgaaaaaag
    11401 aaatagcata gttagtgaag aaaatgctaa aaatttagac gataaggata gaaaagagct
    11461 tacaaagatt agaaatcttt gtgaccaatt tgtgaatgca agtgtttatg taatagcagc
    11521 acctatgtgg agtttatctt ttccagcacc acttaaagag tatatagatt gtataattca
    11581 agatggaaaa actatatctt ttgaaggcaa tgataaacca caaggaattt taaatgatat
    11641 tgatagaagt atggtatata tacaatcttc tggtggacac ataccatggg tgttgaagcc
    11701 agttatgaat aaagggttaa attatgtgga aagtattatg aaatttatag gaattaaaaa
    11761 atttgatgaa ttgttagtag atggaactgg aactagtgaa gaagaaagac aagctgcaat
    11821 agaaaaagca tctgaaaaaa tagatggtat aatagatggg atgaaatttt aattataata
    11881 ttttattaaa aatataaagg aaaaacctct tttaatgtag aaacttatat tgaaggaggc
    11941 gagaatcatg cgagaagaaa aaagtaatga aaagtatgat tgttattggt gtaatcaaga
    12001 gaataatttc tgtgtagaaa taaaagataa tatagtcatg atagatgatg gcactggtac
    12061 gttaaaacaa gccgttttca tagggtataa acaaatccaa attaatttaa actgttcaca
    12121 ttgtcaaaac ttaaatagaa taaaattaaa tttgtagaat tttaaaatgg aaattattga
    12181 aggaattaaa aatagaaagt gttaatattt agactgaaaa ttatatttta tacaataaaa
    12241 gtttatttaa ataacattgt attttaatat ttattttcag tcttacttta ttaaaattat
    12301 tttttggtta tgtctggaaa atatctgtct actaattttt ctgaaatatt ttttaattgg
    12361 tcagccattg aagtcagact taataaggct atcaataaat catctttatt ttctggattt
    12421 atactaatat tttcaaaatt agactctata aagtccggta tattctttct ttgattttct
    12481 tttattaaca tgaatttttc gtaacatgaa actaaatctt tttcactgaa attatccttt
    12541 tcatacacac catgtaagat tcttttaatt tcttgtattg taagtgtatt tttcatagaa
    12601 taaataatta tcatctgtaa aatttgttct ttagtatact tttttccctt aactggtttg
    12661 attaatccct ctttgctgta attatttatc atggttttag taagaatttt atcactttca
    12721 aatcttttat tagcagaaag cttattgtca attagagaaa taatttgatc catatataag
    12781 tcaatagatg gaatatcgtt tgaagttata tctgcattat ttagtgtttc tagtattata
    12841 gtattaagtt cattattcat catatgttac ctccataata tatataatac actaaattag
    12901 aaagtaaaac aatattaagg cttgtaaaaa aaacataaag tggtataatt aaatatataa
    12961 tatagtaatc aaaactatat ttatgaatct gatttttaaa atacataatt gtaggaggac
    13021 gaaatgtatc aatttttttt aaagggtaga gacccaataa gcagtttaac tcattttata
    13081 ggtgcttgtt tatctctttt agcaactata attttagttt ttcaatctgt tacattacag
    13141 gaaacttctc tcttgatgat agtatctgta tctgtatttg gactttcact aattgcttta
    13201 tatagtgcaa gttcgtatta tcatttttta aaggggacac cagaacaaga attgtttttt
    13261 agaaaagtag accatgcaat gatttacgta ttgattgctg gctcttatac accaatatgt
    13321 ctgaatttta tggaaaaaaa agaaggcata atatttgtta cagcaatatg gatagttgca
    13381 tttattggca taataataaa aatattttgg atggatgcac ctagatggtt atctaccagt
    13441 atatatttac ttatggggtg ggcaattgtt tttgatataa atgcattcaa ttcaattcct
    13501 aaggattgcc ttagactatt aattatggaa ggtgtatctt actctatagg tgcgattatt
    13561 tatataatta aaaagcctaa tataagtcct gagtttggat ttcatgaaat atttcatatt
    13621 tttattatga taggttcttt atttcacttt ttagcagttt tattgtatgt tttatagttt
    13681 taaaagattt gattaattga tactaaaatt ctacaatgaa taaaatggaa ctgccttata
    13741 tagattatat cttagacaat aagacagttc cttattataa atattaaatt ctacttataa
    13801 taaaattttg ataaaaatag tccattcaaa atgcctattc cataattcct ttaatgtact
    13861 tttcatttac gacatatagt ttgaatttat gtactataat tatatcctct aaattctctg
    13921 aaacactaac aattctgaca catttgtttc taatttctac aggtaaatct aaatcatttg
    13981 ttaaattcac atagtcatca tatttataca taaaatcatc cctttacatt aagatatact
    14041 tattatacta tattttaata agttttttta aattattaca atttttcccg aaatttcatt
    14101 ttttatacat aaaatttcca aatta
//
LOCUS       DBGJFU010000097        14044 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.097, whole genome shotgun sequence.
ACCESSION   DBGJFU010000097 DBGJFU010000000
VERSION     DBGJFU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.097"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            250..1983
                     /locus_tag="ACOIAS_002728"
     CDS             250..1983
                     /locus_tag="ACOIAS_002728"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2702430.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   2375..2933
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2444..2472
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(3463..3567)
                     /locus_tag="ACOIAS_002729"
     CDS             complement(3463..3567)
                     /locus_tag="ACOIAS_002729"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGZ2702431.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            4192..4437
                     /locus_tag="ACOIAS_002730"
     CDS             4192..4437
                     /locus_tag="ACOIAS_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702432.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
     gene            5294..5881
                     /locus_tag="ACOIAS_002731"
     CDS             5294..5881
                     /locus_tag="ACOIAS_002731"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702433.1"
                     /translation="MDIVLLFYQKFRIPSYYYSIFQQSTINNNKFVAPNNVTLVERCY
                     RYIKDRECSFSPNTIRNRKNMIKNQIEVFFKDMKLIDITPSILQSYINNIYNEHMLNS
                     TKNQVDFIKSVLKESYRLKEISENICDFVTTPIKKNSSTSKLYTKQKAQLLLEKSKNI
                     PIGIPIFLMLTLGLRFGEAVSLIWSDVDLDKKSHM"
     gene            6622..7977
                     /locus_tag="ACOIAS_002732"
     CDS             6622..7977
                     /locus_tag="ACOIAS_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2702434.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(8095..8754)
                     /locus_tag="ACOIAS_002733"
     CDS             complement(8095..8754)
                     /locus_tag="ACOIAS_002733"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HGZ2702435.1"
                     /translation="MLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTISEEH
                     GKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAYGDI
                     DIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGEEFL
                     GKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGYLEI
                     K"
     gene            complement(8830..9258)
                     /locus_tag="ACOIAS_002734"
     CDS             complement(8830..9258)
                     /locus_tag="ACOIAS_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2702436.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            9410..9958
                     /locus_tag="ACOIAS_002735"
     CDS             9410..9958
                     /locus_tag="ACOIAS_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HGZ2702437.1"
                     /translation="MYTNFNIDFNNFNKKENAIKFMLMGVLLIILGLLCLTFKTLGIK
                     LISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVLIIACILLFVFPQKIQ
                     SIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGICLILSPLFLSRFIVSI
                     MSFFIILFGLVLFFSGNTARKY"
     gene            10077..11012
                     /locus_tag="ACOIAS_002736"
     CDS             10077..11012
                     /locus_tag="ACOIAS_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGZ2702438.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(11283..12227)
                     /locus_tag="ACOIAS_002737"
     CDS             complement(11283..12227)
                     /locus_tag="ACOIAS_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGZ2702439.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            12545..13975
                     /locus_tag="ACOIAS_002738"
     CDS             12545..13975
                     /locus_tag="ACOIAS_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2702440.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         5341 a         1898 c         1737 g         5068 t
ORIGIN      
        1 tgattattaa tattgttatt ttaaaataaa ttaaaattat ctttcttttt ttaaacttgt
       61 acattagttt ataaatatgt atctatacaa gatattgaca ttaagtactg aagcatatat
      121 tgtttcagtt cttttttgtt aaaattaact tatttaacaa agatatcata gtttaacaaa
      181 gcttttaatt taaatatatt gatgtataat ataattaaaa tataatagca aaaagaaagg
      241 aggaatatca tgagcatact cctaaaaaaa gcacccaaat tagcaaaaca tattataaca
      301 agtttttata taaatcggga tattgatgaa gtattaaaat acctttgtga aaatgttaca
      361 tggatcggac caggagaaca ggaatttctc acttctttta atgaaataaa aaattatttt
      421 tatgcaggtc aagatgaaat tccttcttgt gatataaata atgatatatt tgaaactgta
      481 agtgaagatg agaatagatg tatggtatta ggaagatata ctgttcgaac taaagaaaat
      541 actcaaatga ttttagaggt aaatcaacgt tgtacatttg agataattga ggatagagga
      601 aaattattag taaagcacat gcacatatca aacccttatg gtgaaatgca acttgatgaa
      661 tactttccca ctaaaattgg aactcaaagt tatgattatt tgcaaagatt actaaaggaa
      721 aaaacagaag ttattgaaat gattacaaat aatatcaatg gtggcttaaa aggtagcaat
      781 gatgataata catactcatt tttctatgtc aatgaaggat tgcccaaaat attaggatac
      841 acatatgatg agttcatgga aatgagtggt ggttcagcag tgggtgctgt atatccacca
      901 gacttaccta aagcactaga agattgtgaa cgatgttttg caaaaggacc tacatattca
      961 tctgaatata gaatccgaaa aaaagatgga acattaatgt gggtattaga ttctggaatg
     1021 aaatcattaa atagtgatgg catagtaaaa attaatagta ttattactga cataacccaa
     1081 ctaaaaaaca tagaatctga actaaaatta gaaagagagc gatatcgtat tgctttgcaa
     1141 aatattacag atatcatgtt tgagtatgat attgaaaacg atacccttat aaaatatcaa
     1201 cgtgtagaaa ttgacaaaaa aattgaatta gaaaattttg aaacaaaaaa ttactccaaa
     1261 ctattagaat ctggtaaaat tattcacctt gatgatattg gaaaactact agaagtactt
     1321 catggaaatc ttcgtgaaac aattgagata agacaagtaa attcattaac taaaaatgaa
     1381 tggagatgga ttcgtgttca atgttcagtt atatatgata gtgaccataa ccctatcaaa
     1441 acaataggcg ttttaaaaga cataactgaa gataaatcta aattagaaag cttaattaat
     1501 caggctcaac gtgacccact aacacaatta tataatcaaa gagtatcaca aaatcttatt
     1561 gaagagtatc tatgtagttc tgattccaaa aataatgatg ctctattagt tattgatatt
     1621 gatgatttta aaactgtaaa tgatactttt gggcatttag agggaaatga agttttagtt
     1681 gctgtatcaa aaatactatt acataacaca catgataaag acatagttgc tcgaattggt
     1741 ggagatgaat ttacaatatt tataaaatca ttaacaaagg atttaataat aaaaattact
     1801 aatgatattt taaatgatgc aagtaaaata aaagtaaagg acaatcataa aataacatta
     1861 agtattggta ttgccttcac tgatgattca actaaattat ataaagattt attttctaaa
     1921 gctgataaag ctctttatct atcaaaagca gatggcaaaa attgttatag tacatacgaa
     1981 taataaagct atttatacga ccaagaatga acctttaaat ttaatttcat agtctaacaa
     2041 tcaaagagtt gaagctaatc ttatagctag tcaactagtt cctagaaaaa atttaaacaa
     2101 tatatttatt gatactgctc atttttctaa agagcaaatt tatataaaaa attaaacttt
     2161 tatgtacata cagatactaa tgataaacag ttatcattag tatcttctat cttaaaacaa
     2221 aatgataatt tatctatata aaaaagtgct ttgttgacag agttcacttt tttatatagg
     2281 tacagaattt aagtataaat acatgtttta ttctatacat tttagttaat gtaaaatttc
     2341 ttgattgtac tctctaactt ttcaacatta tactatttat ataccactta gttaatataa
     2401 accccttagc tggttctagt gctttttcta tgattttagt catatttaca taccacttag
     2461 ttaatataaa accaatttag atgatgtata tgtgcaaaag gaagaaataa tttacatacc
     2521 acttagttaa tataaaacgc tactaaactt gatatacatg accattataa taataaattt
     2581 acataccact tagttaatat aaaacttcca taaaaacatc ccctttttat tcttcttcct
     2641 tatatttaca taccacttag ttaatataaa acagtctttt tttaatgcag agatgcaaga
     2701 agttttataa tttacatacc acttacttaa tataaaacta tcagtattta agtaataatt
     2761 taagtaatat aggatttaca taccacttag ttaatataaa acggtcacta caacttatac
     2821 ttccagtcta acgatttgaa tttacatacc acttagttaa tataaaacat attttggatt
     2881 tattaatata aacataatta aaaaatttac ataccactta gttaatataa aaccccaaaa
     2941 taaactgggc attttcaata cttacacata tgcaactctc ttaaatttgc agtgaaccat
     3001 gagtagtgca aatgataaag cttatcatat accctcaaca ccttatattt caagcgttaa
     3061 gctctacttt gtaacaaata tcgctcactg caaaactatt tacattttta ttatatcata
     3121 aatatattat ttttgaatat ctataccaat ttgtggtata ataaaagcaa gaagaactac
     3181 aatctattta gacgtagagt gaagttcaaa tactaatgtt tattcttttt gaacttgatt
     3241 tttagttcaa aactaaagtc actcctgcct gtgtgaacta tacccgctgt tttatacagt
     3301 taaaacatgt aaaaatagtt ataaagtact agtgtaactc attagtactc tttttattat
     3361 attcatataa ttaatatcta tattttaaag tttactatct aaaaaagagc acgtcaaata
     3421 aaaccctaga tatttaattt ttttagagat acttaaaata aattatgctc tctctgtact
     3481 taaatatttc ctaaattcaa atggagacat agaatttaat ctattttgat atctaaaatt
     3541 attatagtag tatatataat catctattgc tttaactaat tattcatatg tatcaaattt
     3601 ccttaaatag tacatttcta cctttaaaat tccccaaaat ccttccatag gagcattgtc
     3661 aaggcaacga ccaactcttg tataatatat gtatatctaa caccattaag aaatatatat
     3721 tttataatat atatttcttc atttaatgca cttagaaaag caaagaaaat acagattagt
     3781 acacttttta gagaaaaata aaattaatac atctaaaata ttaaaaaata ctttagataa
     3841 tctaaagaca catctcaaag tttttaacta aattataaaa ctaattacaa atgctatgta
     3901 acgataataa ataaaaaatt tacttcgctt aagcgacgtg tcgttgaaat atttcatgtt
     3961 gccaactggt tcgtaagttg ataagaaaag aacactaatt atttagagtt cttttcttag
     4021 ctacaagtgg attataatac ttttaaatct gtgagaaaat catatataat aatgatatga
     4081 acaagtaaag aaaatacaaa aatgacaaaa ttataaataa aatattataa aatcataagg
     4141 tcataattgt tatattgtag aaacaaagta aagagagagg gtggagaata aatgaatcaa
     4201 aaaaaaataa agattagtat taaaatggta attataggta tatttgctgt aattattgct
     4261 tttgtattat ccagattatt cgttgagtat attgatgaat tattaaggtc taattggcaa
     4321 tatcagctat ttgaaagtga gagttcatat atagtatttg ccaattgtat tatgttctcg
     4381 aatatattat tagaaatatt tttaatatat atttgtagaa aatttagaaa aatctaatat
     4441 ggtcaacgta tttcgaatcg aatacttcat gattatttag tctattgtgc tagttaaagt
     4501 ttaattccca tataaagtaa attgatttta gtaaaacaaa tcacccctaa gggttagttt
     4561 atggataagt catccaaata ttaactcttt aatttttgct atagattcat ctttgctcat
     4621 aactttactt ataaaaaatg aaatattata agctagaact tttattctaa ttctagtcat
     4681 aaatcctagc atagagttac tttttttata ttataaataa aactatacaa caaagataat
     4741 atttagtaaa aataactttc attttaacta aacttaaaat tcaattactc tctaaattga
     4801 cgtttaggaa ttagttcaaa ataaaaaaga gtgtatatat tgaaattact acatttcaat
     4861 atatacactc tttttaaatt tctcagtttt ccatggaact ttactttatt tatttctgta
     4921 ttagttgtat tttttatgca tttttcttta aaatcatatt ttctgattca actcttatat
     4981 tataaaattt ctttactaaa ttattctctt tttctatatc ttcatatata ttttttaatc
     5041 gccttttacc taatctttta tctaacatta ctaatgattt aactaatata ttatatgatt
     5101 ttatcgcatc ttctattgag atatttaaac atatatttac tgcttcataa aaatcaaatt
     5161 cattaaaaaa tccattttta tttacaatct tatatccctt tgcatctctt tctaaatact
     5221 cttcctaaat actcttcaaa actttcatca tctaatctaa atcctatatc actatgaaaa
     5281 ataggttttg catttggata tagttctatt gttttatcaa aagtttcgaa taccaagtta
     5341 ttattattcg attttccaac agtccactat aaacaataat aagtttgttg ctccaaataa
     5401 tgtaacactt gtagaaagat gttacagata tataaaagat agagaatgta gtttttctcc
     5461 taatactatt agaaatagaa aaaatatgat taaaaatcaa attgaagttt tctttaaaga
     5521 tatgaaatta atagatataa ctccttctat tttacagtct tatataaata acatatacaa
     5581 tgagcacatg ttaaattcaa caaaaaacca agtcgatttt ataaaatcag tattgaaaga
     5641 atcttataga ttaaaagaaa tatcagaaaa tatttgtgat tttgttacaa ccccaatcaa
     5701 aaaaaatagt tcaacttcta agctttatac aaaacaaaaa gctcaattat tattagaaaa
     5761 atcaaaaaat attcctatag gtataccaat ttttttgatg ctaactcttg gattacgttt
     5821 tggtgaagct gtatcactta tatggtcaga tgtagacctt gataaaaaat ctcacatgta
     5881 aatcaaacat tagtttatgt atacaataaa ataatcttca aaggccctaa aacccctaga
     5941 ttcaaaagaa gattatttgt tcctgatgaa cttataagtt tgtttaaagg ggaaaaatta
     6001 aaacaaaata agttaaaatt acagggtata ttgaaaaatg aatttgattt gatatgctta
     6061 aataaaagat tcaacccgtg agttcaacca acttttgttg ttcattttaa aaaactatta
     6121 agagataata atcctagaga tataaaagta catgaattaa tacatgcaaa tgcgactttg
     6181 atattacttt caggtactaa cataaaagct atttataaaa tattagtaca tgcagatata
     6241 aaaatagcta tgaatatata ttccatattc tcacttatta gatgaaatga ataaagaaac
     6301 atctgaaaat ataagcaaaa tactattcaa ataatgttca gttttatgtc aattaaatgt
     6361 cactcaaagt gctttttttc catttgtcag ttaaatgtca cttaaagtac ctttttccta
     6421 tttgtcagtt aaatgtcagt aatatataaa taagatatat ttataagacc cttaaaatca
     6481 ctattattta ataaaattta taattatata ctatttgctg ttgacattgg tattatacca
     6541 tagtttatca tttatattag aataaaatta ttaattaata gtttcgtaat tttataatag
     6601 atttacaggg agtgataata aatggataca aaaatatcta taaacgaaaa catgagtcta
     6661 ggtaaaagat tttttaaata cctagcacca tctgtagttg caatgtgggt tttctcacta
     6721 tatacaatgg ttgatggaat atttgtaagt aaaggagttg gagaattagc tttagctgct
     6781 gtcaatatat caatgccttt tattaatttt atatttgcag tttctctatt attttcaact
     6841 ggtgcatcta ccataattgc aatctatctg gggaaaaaag atattaaatc tgctaacgaa
     6901 gtcttttcat ttaacttagt atcaataatt atattatcaa taattatatt agctataact
     6961 ttttttaatt tagatagatt agcactattt ttaggagcta cagaatctac aattggtatg
     7021 gttaaagatt atttaggaat aataatattt tttaacggat tctttatagt gtcttattct
     7081 ttagaagtta taataaaaac tgatggcttt ccaatattag ctactgttgg tgtaataata
     7141 tctgctctta caaacatcat acttgattac ttgtttgtaa ttgaatttgg atggggtgta
     7201 aaaggtgctg gtattgcaac tggtttgtct caagtctttt ctactatttt cttcttaata
     7261 cattttttaa gaaaaaactc tactttgaat ttttcaaaat ttagaattga ttttaagaca
     7321 cttagaaaaa ttgtatttat aggttttcct gattcaacta ctgaattaag ttgtggtata
     7381 gttgttttac tttttaactt aagtcttact aaatatattg gagaaaatgc tcttatttat
     7441 tatagtgtca taaattatat aaatacatta gtacttatga ctatgatggg tataactcaa
     7501 ggtatgcaac cacttactag cttttattat ggtgcaggaa atatagataa tgtaaaaaaa
     7561 ttattaaaaa tggggataaa agccacaata atagcatcag ttgctgtatt tgcaatttgt
     7621 atggcttttt caggaccaat agtctcttta tttatacatc cagaagaaac aatgctattc
     7681 aatgaaggag ttcgtgtatt taaaatattc tctatatctt tcttattggt aggaattaat
     7741 gtaataattt ctggattctt tgtatcagta gaaaaaccat caatttctac tgttatatct
     7801 cttggtagag ggcttgtaat agttgtttta agtctaattt caatgatact aatatttggt
     7861 ggacaaggaa tatggatgac tactattgtt tctgagttta tatgtcttat tttatcatta
     7921 gtttttctaa aaaagaattt ttctacatta gactctaatc taaataaggt tgcttagatg
     7981 ataagagtga caatatatga aaatatctaa ataaaaaata cctattatac aatcttgcaa
     8041 atattaaacc tttgcaaaaa ttaggattat ataataggta ttttatttat gtaattattt
     8101 tatttctaaa tatccatagc catcttttat taaagttcct tttacttcat attctacagg
     8161 ttttttaaat attggaccat ctacgacaaa tgtatgatac tctccatgtt ctccacatgg
     8221 gtctgcacca gcatttttta tcttctccac aagttctcta gttaattttt ttcctaaaaa
     8281 ttcctctcct aagtgcttta agtttacttt tgttacaaca ctacaaaatc cactgtctat
     8341 aaattcatat actaaatcct ctctactctc ttgccataat ggtaaatcta ctttcatacc
     8401 aactgcttca catctatcag tatcccattt tctatgactt tctatatcta tatctccata
     8461 tgcacatatc gttgctccca tgtttttagc ttttattaaa gccctctcaa agctctcttc
     8521 atatccttct acgccacact cagcaactaa taaaggtaat cctatgttgc tacttacttg
     8581 cttaagcata ttatattcta aattatgtgt ccatgacttt ccatgttctt cacttatagt
     8641 tgtcaagagt gccacaggct tatatccttt tttcaacatt cgatttaatg ctaatatact
     8701 atccttacca ccactaaatg acattacaaa tttttcatca gccaatttta acacctcttt
     8761 catctaactt tgttttatta acataattat accaaatttt tgagttgcat agcaaattat
     8821 attaaattgt taattcacta taaagaatgt ccctaatatt aaaagtataa cccctatgta
     8881 tttgtataaa tgcattgttt cttgtcccaa aattacgcta tcaataaaaa atccaactaa
     8941 aacttgggat acaagagtta atataagtgt atttgatact cctaaatttg gaattgcctt
     9001 tactgtaaaa tatattatta gacctccaaa tactcctcct aataacaact tataattaaa
     9061 accatttata gatactagac actttaagtc acctgtaact attatacttg ccaaaaaaaa
     9121 tattgctcct acagttaagc ttataaaggt ggccactaat gctgttgtac tttttcctag
     9181 ctctccattt ataaaagctt caagagctgt agaaccacct gctaataatg caaatattat
     9241 tgcccataat tttgtcatgg acaaacctcc ttctatataa ttctattaat agaagtttat
     9301 gatacaacct attaattaat tactaagtat agtttaattt ttatacacta ggctttattt
     9361 ggatatgaat ttctgttata attgttctat cgattttgga ggtgatttta tgtacacaaa
     9421 ttttaatatc gattttaata actttaataa aaaagaaaat gctattaaat ttatgttaat
     9481 gggagtatta ctcattatat taggattatt atgccttaca tttaagacat taggtataaa
     9541 attaatttct tggacttttg gtattgcact tttattcttt gcatatttaa atttaaaaaa
     9601 tattaatgag ttaaaaagat atgctactaa agaagaaatt aaaccctcta ttaatatcca
     9661 atgggttcta ataattgcat gtatattact ttttgtattt cctcaaaaaa tacaaagcat
     9721 attttcatta tttttaggat tttatttaat ctttaatcaa ctagtagcac ttgtaaatag
     9781 taagaataat ccttattcta aatttacaac ttggaatata gttaagattc tatttggtat
     9841 atgccttata ttatctccac tttttttatc tagatttata gtatcgataa tgtctttttt
     9901 cataatatta tttggactag ttctattttt ctctggaaat acagcgagaa aatattaatt
     9961 attttctcgc tgtattttta tattacttaa aatagaattt gtttaagtta tctatttgtg
    10021 gtaatatgtt ttttgtacaa gctcagcgta caattttatt agaaataggt gattttttgg
    10081 ataaatttaa atatgctttt gataataaaa gatatcatac ttggaattac tatcttagaa
    10141 atacttttgg agaaaaagtt tttaaggtat ctataaatgc aggatttact tgcccaaaca
    10201 ttgatggaag tcttggttat ggcggatgta cctattgtag caaagaaggc tcaggtgatt
    10261 ttgctggaaa ccctaaagat aatcttatca gtcaatttta taatataaaa gaaatgatgt
    10321 taaaaaaatg gcctcatgca aagtatattg gctactttca agcctataca aatacgtatg
    10381 ctcctcttga agttttaaaa gaaaaatatg agactatatt agaacttgaa gatgtcatag
    10441 gtctttcaat atccacaaga cctgactgtt tgccagatga tgttgtagaa tacttatctg
    10501 aactaaataa aagaaccaat ttatgggttg aacttggact tcaaacaatt catgattcta
    10561 catctaaaat tataaataga ggtcatgatt ataaaacatt tgttgaaggt gttgaaaaac
    10621 taaagtctaa aaatataaag gttgtcgtac acattataaa tggtcttcct ggtgaagatt
    10681 ataatatgat gatggaaaca gcaaaggcag ttggtaaaat gggtgtagac ggtataaaaa
    10741 tccatctatt gcatgttata aaagatactc ctatggaaaa gatgttacaa aatggtatgt
    10801 taactcttat ggaacaagat gaatatatca atcttgtctg tgaccaatta gaaatacttc
    10861 ctgaaactat gataatccat agactcactg gtgatggtaa gagggatgaa ctagtaggtc
    10921 ctatatggag cctaaaaaaa tgggaaattt taaatcaaat tgatgatact ttaaaggcta
    10981 gaaattctta tcagggctgt aagtttgttt aatacaaaat aataactata gaaatggtta
    11041 tataaatata gtcctttcta tagttattta gtatttacaa aacaaaatat taatttgtac
    11101 atttatacaa tatataataa aaaatagata gtctaaactg tatctactta tattttattt
    11161 gtactgtcca ctttattcta taaaatttat aaatagttat tttttaatac caaatataat
    11221 ttggtataat ttttgttgga ctctaaaatg tataccaata aatccttttt aagtatataa
    11281 ttctaaatac tttcagtttt ttcaatctgc tttaacttgt tggacttata aaatattctt
    11341 atttcatcat aagaaagatt aagtgctact actgcagata ttaacatcaa catcactcca
    11401 aataacttcc ctaaggaaaa actttctcct aatatagtgc aaccaattat tactgaacca
    11461 actaattcta ctgatgccac aacacctgca actgacaact ctacaccttt agatatacct
    11521 gctgcataaa aaatatatgc aagtgcagct ggaacaatgc ctaacattag catacaagat
    11581 aaaacatcta aatccttagc ataatttaag atttcccaag gtcttgaaga aggaatcata
    11641 aatatagctc caaataagaa actgtatact aatattgttg aacttgaaaa ttcctttaaa
    11701 gcatttttac taatagttgg cataagtgca tatgctattg ctgataatac cccaagtaaa
    11761 agtccaaaag cgtttaaacc ttgaaaatcc aaacgtccac cagtcactgc cataattgca
    11821 ccaataaaac aaagaataag tgaaaataat ttgcttctag taatattttc tttataacat
    11881 atttttgaaa atattgctaa aaacagtggt gatgtatata gtaaaactgc tgcaattgat
    11941 acacccgcaa tgtcaattgc tttaaaataa cataaattaa acattgcctg acatataata
    12001 cctattatta ctgaatatat aattcctttt ttgcttatct ttaagatttg tggtgtcctt
    12061 atagatgaat atacgcctaa aactataaat ccaaaaaaca atcttgtaaa tgctacttgt
    12121 tctgatgtca gtccatttcc cattaaaaac tttccaaata atccaagtgt agcccataat
    12181 aaccctgcta ttgctataaa tacataccct ttactttttt ctcccatttt aaattccctt
    12241 ctatttttat ttagtcaatt tacactcttc tttttaagca aaaactatgc catcttaaat
    12301 caatatatat cgactctaaa accctagtct ttccaataat actaataaat tttcaatttt
    12361 tttatattta tttttcactt actttaagtt gatttagact tattttaatt gtaaaatgag
    12421 ttatatataa cttatttaaa gttaaaatag actgaaactt atatataaat atcaaatttt
    12481 aagtatatta atcaattaaa cctattttaa tatatcatta aaaatttagg aggaaaatat
    12541 aaaaatgaat ttagatttgg aattttatca aaaaatatta gaagcttccc atgatgaaat
    12601 atgtgtttct gatgataaag gaataataat atattgtaat aaagcatttg aagaaaatta
    12661 tggtctaaaa aaagaagata ttttaggaaa aaatgtttct tttttagaag actctggata
    12721 ttcaacaaaa agccccatac caattgtatt aaaaacaaag actaaatttt cactggaaca
    12781 agatactcaa acagggaaaa agcttatcat aactgctacc cctatatttg atgaaaatgg
    12841 aaacttagaa tttactgttg aaaattgtag agatataact gaacttaata atataaaaaa
    12901 taaacttgaa gacacaaaaa agcaagtaaa aaaatataaa tctgaagtag aaacactata
    12961 cagaactgca cttagaattg aggatactgt aattatggac ggaatagtta tgagaccaat
    13021 cataaacaca gtaaatcatg tttctaaaac ggatgtaagt gtacttttat tgggtgaatc
    13081 tggaacagga aaaagctcac ttgctagata tatacaccac aacagcaata gatctaatgg
    13141 cccatttata acaataaatt gtgcaactat atctccacaa cttcttgaat ctgaactttt
    13201 tggatatact tcaggtgctt ttacaggtgc aagtactaaa ggtaaagttg ggctcgtaga
    13261 attagctaat ggaggaactc tttttttaga tgaaatagga gatataccac aaaatctaca
    13321 ggcaaaattt ttgcaattaa tacaggatag aacttttact ccagttggga gcttaaaaaa
    13381 taaaaaggtc gatataagaa ttatatctgc tacaaatgtt gatttagttt caaaagtaaa
    13441 agaaaaaaag tttagagaag atttatacta tagattaaat gttatagaaa taaaattgcc
    13501 tcctcttaga gaaagaaggg ataatctagt ggaaattata aaatattatt ttaataggta
    13561 ctcaagtgac ttcaatctaa ataaaactat ttctaaagaa gctatggatg ctattgcaaa
    13621 ttatagattc ccaggcaata taagagaatt acaaaatata attcaaaaaa tcttgcttac
    13681 ttgtacagat aatcatataa caatagatga cttaccaaac attcttacaa aaaatataca
    13741 tattaccaat aatggaaata aaacacatat atcccaaatt aataaggtta ttatatctga
    13801 ctcaaaatct actaattata aaaataaaaa ttttgacact ttaattaaag agtatgaaaa
    13861 aaatataatt ttggatgctt atgaaaaatt tggtagttct tataaagtag ccaaacacct
    13921 tgaaatctca cagtctaaag ccaataggtt aattagaaag tatactaata cttaatttta
    13981 aatttgataa ataaaaactt aacactattt acatattgta aatttggcca tttactgtta
    14041 ttat
//
LOCUS       DBGJFU010000098        13808 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.098, whole genome shotgun sequence.
ACCESSION   DBGJFU010000098 DBGJFU010000000
VERSION     DBGJFU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.098"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            378..1028
                     /locus_tag="ACOIAS_002739"
     CDS             378..1028
                     /locus_tag="ACOIAS_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HGZ2702441.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1231..1902
                     /locus_tag="ACOIAS_002740"
     CDS             1231..1902
                     /locus_tag="ACOIAS_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702442.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1899..3029
                     /locus_tag="ACOIAS_002741"
     CDS             1899..3029
                     /locus_tag="ACOIAS_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2702443.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3280..3402
                     /locus_tag="ACOIAS_002742"
     CDS             3280..3402
                     /locus_tag="ACOIAS_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702444.1"
                     /translation="MKWYTWLTIILIILLSIPLKIKFIKWWDRRQKNDKGGYDR"
     gene            3399..4244
                     /locus_tag="ACOIAS_002743"
     CDS             3399..4244
                     /locus_tag="ACOIAS_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702445.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4244..5272
                     /locus_tag="ACOIAS_002744"
     CDS             4244..5272
                     /locus_tag="ACOIAS_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702446.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5275..6288
                     /locus_tag="ACOIAS_002745"
     CDS             5275..6288
                     /locus_tag="ACOIAS_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702447.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6805..8055)
                     /gene="cme"
                     /locus_tag="ACOIAS_002746"
     CDS             complement(6805..8055)
                     /gene="cme"
                     /locus_tag="ACOIAS_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HGZ2702448.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8141..8887)
                     /locus_tag="ACOIAS_002747"
     CDS             complement(8141..8887)
                     /locus_tag="ACOIAS_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2702449.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9040..10062)
                     /locus_tag="ACOIAS_002748"
     CDS             complement(9040..10062)
                     /locus_tag="ACOIAS_002748"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HGZ2702450.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10062..12482)
                     /locus_tag="ACOIAS_002749"
     CDS             complement(10062..12482)
                     /locus_tag="ACOIAS_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGZ2702451.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12568..13488)
                     /locus_tag="ACOIAS_002750"
     CDS             complement(12568..13488)
                     /locus_tag="ACOIAS_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2702452.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         4868 a         1799 c         2174 g         4967 t
ORIGIN      
        1 tataattatt taaaactaaa tataaatgtt ggaattattt aggacttgaa attgtctaaa
       61 aataaataca agtgatgaag aggtgatgtg atgagattag atatggatag tatgttatat
      121 gtactaattt atatgacatt attggtatct atcattggaa gtatatttta tttttgtaaa
      181 gacctcttaa atcataaaat taagtatgtt ttaataagag acatatttgt aaatagaatt
      241 acatattctg aagaacgact gcctaaacag tatatagttt ttcagaaata tgatatttgg
      301 aggtattgta gtttgtttta atttgaaatg aatctattat aaattctaaa gtttaaaaat
      361 aaaaaggaga gaataaaatg gatttcattt caagtatatt gcaaggatta ttaagtatat
      421 tatttaattt tacaggtgat tttggaattt ctattgtact tataacttta atagttaaat
      481 tagtacttat gccactatca ttaaaacaaa gattttctgt aaaaaaacag caagaattag
      541 ctgaaaaaat ggaatatata aaagaaaagt ataagaatga tactaaagaa ttggaaaagc
      601 aaatacaagt aagttctgta gaaagtatga agagtatgct gggatgtctt atagttttgg
      661 ttcaaatgcc agtagtatat gctttatatc atgtatgttt agatatgaca aaggaattta
      721 caacaattat aattccatgg attgctaatt taggtacatt tgataatttg tttatattac
      781 cttgtatata tacattaatt atgttagctt caaatttagt gaattatata ccatatttaa
      841 aaataaattc tcaagtaaag tttaacaaac aaatggctgt ggtgactacc ataacaagtc
      901 ttatactaac tgttagaact ccagtagcca taggtctgta ctttataaca agtgcaattt
      961 attcattaat tgaagatatt tgctttagaa tatattttaa tcgaaaaaat agaatagtga
     1021 caaaataata taattattat tagaaatatt ttagcctgct aacatagaaa aagttagtag
     1081 gctattttta tctatgatat caattccatt aagaatagtc taaaaaatac tatcaaaatg
     1141 ctataaaaaa tactataaaa aaatagttga aaaataaata aattaatata aactaaaata
     1201 aagcctacaa aaaagaggtg ataaatagaa atggctaaaa tactagttgt agaagatgaa
     1261 aaaagaatgc aaaatattat tgtagaatat atgcaaaagg gaggatatac ttgtataact
     1321 gctgatgatg gagtcgaggc acttacaata ttaaagagca acaatataga tttaatgata
     1381 ttggatatta tgatgccgta tttagatgga ttttcagtgt gtagagtttc acgtgaaatg
     1441 actaatatac ctattattat attgacagct aaaggtgaag aggaggataa attaaagggt
     1501 tatgaatatg gcgcagatga ttatataaca aaaccattta gcccaaaagt acttttagca
     1561 aaagttaatg ctttgttacg tagatacact actgatattc ccaaaaattc attgagttta
     1621 ggaaagattt ttattatggt agcatcaaga caagtgtatg tagaagataa attgattgat
     1681 ttaacatata aagaatttga attattgcgt ttatttatgg aaaaccctaa tcaagttttt
     1741 tccagagaga aacttttaaa ttgtatatgg ggatatgatt ttgaaggaaa cacacgaact
     1801 gtagatacac atataaaaac cttacgcaaa aagcttggta gtgaaggaca ccatatagtt
     1861 acattaattc gttcaggata taagtttgag gttaaggaat gaatagttta agaaagatta
     1921 tagaaaaact gaaaaatgta gatatttcaa attggattaa acaaaatttt ggactatatt
     1981 cagttagaaa aaaagtgttt ttactttcta aattatcagg agcgttaatt atattatttt
     2041 atctggttgt tgaagaattt tcgataaact ctaaatttga attttggatt tggtttataa
     2101 tactggttat atttattatg agtattgatt tcttattggg aaaatttatt tcagaaccaa
     2161 tcacatctat aaataaatct gcaaaaagta tgtcccaact tgatttttct aatccatgta
     2221 ctgtaaatac taatgatgaa tttggagaac tttctaggag cctgaataca atgtctacaa
     2281 atctacaaca ggcattatca gacctagaaa gtgcaaatat tcaacttgaa aaggatgtaa
     2341 ataaggaaag aatgctattg gaacaaagaa aggagttggt agatacaatt tcacatgaaa
     2401 tgaagactcc acttggcatt atacgtgcgt atacagaggg gcttatagat gaagttgatg
     2461 aagaaaaaaa gaaaaattat atgaatgtta taatagaaga aactgataga atgaacaata
     2521 tgattgtatc attattagac ttatctgcat tggaagcagg agtttctaaa cttaacccag
     2581 agcgttttga ttttatagag tttgttgaaa ctgtggcagg tcgtctttta attgatatac
     2641 cagatgccaa tttttatttt acatatgatt tgccagagta tgaagttttt gtagtggcag
     2701 ataaaatgag aatggaacaa gtagtagaga atctagttat taatgcaaag aagcatgttg
     2761 tatataatgg taatttagat ttatcgatta catgtgaaaa tggcttgctt ttatttgaaa
     2821 tttataatga tggtaggttt atagagcaag atgagatttc aaagatttgg tctaagtttt
     2881 atcgtagtgt tcaatctcag cgtacaggag gttcaggtct agggcttgca attgtttcac
     2941 aaatacttac aatgcaaggg ttagaatatg gagtgaataa tcgtgataga ggagtagagt
     3001 tttattttat gattccaatt gatgaataat ttagtattaa tttataaatt ataagtatct
     3061 gatgtaaaat atttttattt gtatacgtaa gtatttaaag agaataaaat aaaaaaactt
     3121 aatgaaaaag cttaatgaaa aagtctaata aaaagctttt tattatttca cttcgatttc
     3181 acaagagaga cactctgatt tcaccaaata gaaataaaat aggttaaata aattaaaaaa
     3241 tagaagcgta attaaataag gttacaaggg ggagtactta tgaaatggta tacatggttg
     3301 actattatat tgataatatt gctttctatt ccgcttaaaa ttaaatttat aaaatggtgg
     3361 gatagacgac aaaaaaatga taaaggaggt tatgatagat gataaaagta agagagttaa
     3421 aattttctta tggaaaagat aaacagatat tgcatggact taattttgaa gtaaaagaag
     3481 gagaaatctt tggattcctt ggacctaatg gttcaggaaa atccacaaca cagaaaatct
     3541 taaatggtgt tttaaaaggt tacaatggtc acgtatccat ttttggtaag gaagttaaag
     3601 catacacaga atctttatat caaaagattg gtgttttatt tgaatttcct tatttatata
     3661 ccaatttaag tgcaattgat aatttagaat atttttcgtc tttttatcct aaaaatcaaa
     3721 ggagagacat atatgaactc ttagatttgt tagagtttaa gaaagagttt ataaataagc
     3781 ctgtatcatc ctactcaaaa ggtatgaagc aacgtattag catggcaaga gcattggtaa
     3841 gtaatccaaa gttgttattt ttagatgagc ctactagtgg acttgaccca tctggagctg
     3901 tactatttcg taagattatt gaagaagaac gaaaaaaagg aacaactatc tttttgacta
     3961 ctcataatat gctagatgca gatttaatgt gtaacaatgt ggcttttatt gcagatggaa
     4021 agattatggt aatagataaa cccaaaaatt taaaaatgaa aaatagcaat aataaagttg
     4081 aagtggaatt tgtatataat ggaaatcgag atattgaaat tgtagatata caagagttag
     4141 aatctggaat aacttttaaa tatgatgaaa tcttaagtgt acattcaaaa gaacctacac
     4201 tagaagaagt ttttattaaa tacacaggaa ggatgttagt gtaatgtatt ctaaattttt
     4261 taccttagta aaaaaagatt tttggatgat gatttatggt aaattttttg tgctgacact
     4321 tggctctctc atactttatt cgtgttatat taattttggt tatgtaaagt ttatggatga
     4381 agatagttat cacaataatg tatatctata taatccagag aatataagca tgagtgcatc
     4441 tccattaatt aatcaagttt cttctatagg tgaattaaaa gcaaaacttg atgaagatac
     4501 aagtgcagtt tgtattgata tgagttccag taagccgaaa gtaattttac atgaaagtat
     4561 taaaggagtt gacaattata gagctgacta tgcattgtca ttattatcat cagaaaaaga
     4621 ccataatagc aaaatatcag acaacaatgc aataattcaa attaatgata aggatacatc
     4681 tgagataaaa caaagaaaag acatgacttg tgagatttta ttctttgaaa ttgtagctat
     4741 tggattttta gggattgcct ctttattatt taaagaaaag cagatgggag taatgcgtat
     4801 tactggtatt ttacctgtaa aaagaagtct ttttatatta tcaaagatat ttgctttttt
     4861 tatatgtgac ctatgtttta cagttctttt agtagtattt aatattggat ttttagaagg
     4921 aataaaaata cttccacaag tattagttca agctagtatt ttatcaatta ttatgtcttt
     4981 gattggaata ggatgctcaa tgttattgag agattttaag caattttctc ttatatatct
     5041 tgtggttgct gtattcataa ctacacctat atttctatct gcaaatacat cttttgaaat
     5101 agattggatt aaatatcatc ccttctatca tgtttatagt cagcttaaaa atgcgttttt
     5161 catgaatttt tcaaaagaat tagtttattt tgtagtgtgt tctttgacaa taatagttct
     5221 atttttactt gtaaaatata tttttaataa agaattggtt agggagggat aatcttgata
     5281 ggattacgtt accagttaaa gagtataaaa aaagataaaa tgtgtataat ttccttcttt
     5341 ttaccagttg ttatggcgat tttaataaat tttatgggaa caattgatat atcttcatta
     5401 ggagagatac gttttggtat agtgaaggat acacttcctg tacaaacaga gcagtggtta
     5461 aagaagtatg gtatggtaag tgtatatgac acaaaaatgc atttaatacg tgaaattaag
     5521 gatattaaaa cggacacaat tggagttgtt agagatggta aaggaataaa aactgtgttt
     5581 tatggaaatg agattaatgc tataaaaaat gttggaaaga cattgccaga gatgtttaga
     5641 ctaaagggtg atttaaaaga tgttgaggtt acaatattgc ctaaagatag tattttaaaa
     5701 gagtttcaaa atgtttttat tgctatgatt ttaattactg ctatgtttat gggatgcacc
     5761 tttaatgcaa tgaatattat atcggaaaaa gaagatggaa tatcatttat aaatgaagtt
     5821 ttgccacaga gtagaagtca gtatattata caaaaagtat ttttgggtct tttatgtggg
     5881 tgtttatctt caatgataac tgtatttata tgtttggata tttcaatatt agatatgata
     5941 atgatgttgt tactcgttgt attttcagca tttatttcat cacttatagg tttatttatt
     6001 ggaaagttgt cagaaggtct tatggtagga attgtttata ttaaaatagt aatgatttta
     6061 tttataggta ctcctttgtt aatatatctt ctaggggtta ataccagtgg tatagggaat
     6121 ttatgctata taatgccttc agttgctaca tttaatggta ttatggagat tattaatggt
     6181 aatgtcatta ttatcaaaga tattgtaatt ttggctgtac attgtgtagt atggtttatg
     6241 atatatatta ttttagataa aaaaagaaag ttatctttta gtagataact cagtataatt
     6301 ataattggga caatttaata ggtgatttaa tatactttgt atagtgtaat ttatgtagtg
     6361 caatttgtat actttaattt atgtagtgta atagttagtt ttattttata attattcaat
     6421 ataatttagt aagaagttca gtacaattag agctacttag tggaaaactt aataggtact
     6481 taaactatat aaatacttta ttttcctcca tttgtactat aaatttaatt tatgatataa
     6541 atggaggtat tattatatga tttgataaat ttcatcatta taacctacaa gtaaaggtat
     6601 tattatataa agttaataaa gatagtatca aaatattatt tgctataaag agagggactc
     6661 aaaataaaga taaacctatt tttcaatgtt gaaatgataa aatatatttg atttcatata
     6721 aaaaattttc ttcataaatt aaaaagtcca ttttaagccc cttcattttt aaccagataa
     6781 atcgagttaa actatttaaa tatattactc ggattcattt gacttagata ttccttgctt
     6841 gtaaaattgt tttgaatagg ggatagccaa agcaaaaaat aaaagtataa tccctgctat
     6901 gattattaaa ttattaatag aaattacatc tgccattggt ccgaaaatca tcataccaag
     6961 aggaataaaa ccagtatata taataatagg taagctaaga actctaccta gcatttgtgg
     7021 tgctacatgc tcttgcaaca tagtcatagc tgatgcttga atgattggaa ttgaaaaatt
     7081 cgtaaaaaac ataaatataa aaaattgcca agcttgtgtt acaagaccta gaataattga
     7141 aaaaatagca taggataata ttgctagaaa aaaggtctta tttctatttt taaaaccacc
     7201 aaaaactcct ataatcaatc cacctaaaac catacctaaa aatccaatca tttcaataat
     7261 agacaggtat atataaccct tgccaaatgt tcgttcaata agaagtccta taagaaatcc
     7321 tgatggaaca caaagaagca tgaatgctcc ataagtaaaa agcacttttc taatgaactt
     7381 atctgaaaaa cagtacttta tccctgatac catatctgca aaaaatgaga ctttttcagt
     7441 ttgttttggt atatcatgtt taggaatttt gatgaatatt aataatacaa tagcaataat
     7501 tgcagtaaat acatcaatta aaagaacttg atttatttta ttaccaaatc caagtattgc
     7561 agcagctaca ataggaacta taaattgtac tatagactgt agactactat ttataccatt
     7621 aaaacgcatt aaatgttctt ctggtacaag ttgaggaatc attgcgttta cagcaggtgt
     7681 ttgtacacca cttcctagtg aacgtataac agatattact gatatggcta ttaattcact
     7741 tgttgtgcca tttgatgaca tcataaatag aattagaact aatgtagata tagcaattgt
     7801 tgaatctgat acaattatga gtacctttcg attgtagtag tctgcccata caccagcaaa
     7861 aaaagaaatt atcatttgag gtataaagga gcatacagtt aaaactgtta cccatacacc
     7921 tgatgatgtc tgaagtgtga cataccatat cattgccatt tgtacaagtg atgagccaaa
     7981 taaggaaata tattgacttg ctaaaaaaag aatgactttt ttcttccatg tagaattagt
     8041 atttttattt tccatcctat tcctcctttg atttgtgtgt tgaatttttc ttgctcttaa
     8101 ggataacaca caaatcaaag tctggagtta gagtggaggg ctaggaaaat tgttttttca
     8161 taatttgaag atgagatttc atggtttttg cattatgttc agcttctttc aatgaagtta
     8221 tcagtttgtc acacactgag ataatttcgt catcatcttt tggagtgtca aggacagttt
     8281 taatattagt attaggacta gaagataaag catttaacat tcgtaaaata gctgaaagag
     8341 aatagtttgc acaacgaagt gcgcttataa ttttaagtcg ttttatatct tcatcagtat
     8401 atacacgata accattttgt tttcttttta cttttaacaa accattcatt tcccaatttc
     8461 ttaaagtatc tattgtaacg tttaagtgtt ctgcagtttc ttttctagtt aaaaagagtt
     8521 tatttgtttc tggagtattt ccatttaaaa tctgtgcaac aatagtaagt gcttcctcgg
     8581 cattagattt ttctatttct aattgttgca agtattgatt tacaagacta atagccttat
     8641 caaagtccct attcgctgat gttcttacaa tctcaagggc atcttttctt aatccccttt
     8701 gtaccacttc aattttcaag gctgtacgag caaatctaaa ttgttctata tgaaaatcat
     8761 taaagatacg ataaccattg gtattacgct taggtttagg tattaagttt agttcttcat
     8821 ataaacgtac tgtattagga tgtacaccaa ttaaatctgc tacctctgat gtcgtgtaag
     8881 tgtccattag tattcaactc cttttagata tatagtatta gtcataataa caccaaacaa
     8941 aagtctggtg ttataatgga gggtttggct atctaagagt aattagttta tgaaagagta
     9001 attagtttat gaaagagtaa ttagtttatg aaagagtaat taatctatta tagaagtaag
     9061 tatatatttt tcaatgaact cccatctatc acgaggttta aacactattt tagatggtat
     9121 ggctattact aagtcatatt gtggaataca acaaatagca tttcctccag ttcccattgc
     9181 tgaataccac aaaacaccat cttttttaca tagccaccac agataaccgt attcattact
     9241 attcatttta actgattctt caatccacga tttagatata atacgtttat tattccaaat
     9301 accatcattt aaatacagaa aaccaaatcg caacatatct ctagcactca atgtaagtcc
     9361 ccagccacct atagaataac cctgtctatc ttttgcccaa cccttaagat tttttccaaa
     9421 gaaatcttca aaaccaaaat ctttcatatc ataatctggt ataactttca taccaatagg
     9481 tttaaataag tactcattag caaattcacg agcagatttt ccagtagttt ttgttataat
     9541 agctgaaagt aggtgcgttc cagctgaaga atatttgaaa gtaccaattt cgcctttttg
     9601 accaagttga ttcaaggtat attttatcca atctttttgt ttgcaaagtc tttctaaaga
     9661 ctcagtcata tttttaaaag ggtatggtgc agtcattgta agtaggtgac gtatagttac
     9721 tgctttttta catatttcag aaggactaca agtatattca ggaaaaaaat caacaacctt
     9781 ttggtcaaca tcttttatat atcctttatc tattgcaata cctattaagg ctgatataat
     9841 actttttgta actgatgcaa catgaaaagt atcatcagca ttatatccat tatagtactt
     9901 ttcaaagaca atttttcctt ttcttgcaat aattatacca tttatattgc tatattgtga
     9961 gattattaaa ggttcaagtt gtgaaagctt ttctgaactc ataccaacac ttgaagggtc
    10021 tactttttca aaaattaagt ctaagcaatt gtttttattc attaaagata cctccctgtt
    10081 atagattttg gattttcttt tagagttttt ggagaacctt ctgcaattat ataacctcct
    10141 ttgtcaccac cttctggacc aagctcaaca atccagtcac aaacatttaa tacatctata
    10201 tcatgttcaa caacaattac agaatttcca ttttcaacca attcatctaa taattgaatc
    10261 aacttagaag tatcatatag acttagacca gtagttggct catccaatac ataaaggata
    10321 ttaccatttc gttttttacc aatttctttt gcaagtttga ttctttggga ttcaccacca
    10381 cttaaagtcg gtgtaggttg gcctaatttt atatacccca tacctatttg ttctaataca
    10441 tttaagtgtg aaacaatatt tggaatatct ttgaagaaat gtacagattc agaaacactc
    10501 atatttaaaa tatcatgtat atttttattt ttatatttaa tagataaaga ttcatcatta
    10561 aatctttttc cctgacattc actgcatata tggtctatac ttagattatt tcccaaccag
    10621 accttttcat atccactacc actacattta ggacaagccc ctttggagtt aaatgagaaa
    10681 tgaccagctg taaatccttg ttctaacgat tctggctgac ctgcaaatag tgtacgtatc
    10741 ttatcccata taccaatata agaagcagga gttgagttca tatttttgcc tataggagat
    10801 tgagatatct ctgaatatcc agaaatatgt ttagtaccaa tcagtctatc tgcaatagtt
    10861 tcaacaactg cataatcgtc ttcctcacct atttcactct ctgaagaatt tatttgatta
    10921 ttgatagtat ggttattaaa ttggcttcta agaagaggaa gtagggtgtc tgatataagt
    10981 gaactcttgc cactacctga ttttccagat attccaacca tagcacccaa aggtaatgat
    11041 acagttacat ttttaagatt atttgtattt gcattttgaa tagttataca aggtatttta
    11101 tcagtatgtg taatgttttt attgcttgtt ctattaggca ttggatattt tccagataga
    11161 tactgtccag taagtgattt gtcggattga agtagacctt ctaaatctcc ttgatatact
    11221 aattgaccac cttcaacacc agcttttgga ccaatatcaa taatatgttc tgccatttca
    11281 attgtgtttt tatcatgctc aactactatg accgtattgc ctaaatcttt aagtttcttt
    11341 agtgatttta agatgtctat tttttctgat tcatgcaatc cagctgttgg ttcatcaagc
    11401 acataaatta atgagtccat ctctgaatcg agatgtgaat ttaaaaatag tctttgcatt
    11461 tccccaccac tcaaagttga catttctcgg tataaggata gatgacctaa tcgtgattct
    11521 attaaatgtt gagttttgga taaaatatca tttacaagat ttgtaccaaa tgtgtttaaa
    11581 ttaccttgat ttattaagag tttaaggaaa tcatcaattt ctaaaatcgt catttttcca
    11641 agttctccaa tatgttttcc gtttaaaagt actctacgtg cttcttcacc aattttaaaa
    11701 ccattgcaat ctgaacaggc agtcatagta taaactccat ttaaatcttc gccccttttg
    11761 taacgattgt gaaatacctt tgataaagag gaactttttt taccattttc ataatgacca
    11821 tagagtactt catttcttat ttcttcaggt agttgtaaga attgtatgtc taaataatca
    11881 ttgaattttt tagaaaatac tctcaaatat ccaggtgtaa tctttgctct atctaagact
    11941 tgttttaggg tatcatgctt atcaaggact aatttttcaa tattaatctc aaaatatgca
    12001 ccatgtccag agcacttcat acacattcca tctgaagagt tgtatgagaa ataacttggt
    12061 cttaatcgtt cttcctcata tccacaatta ttgcaaatta gattatcgct tacaggagta
    12121 tcacacatag tacacattat ttgaccttcc actgaataca atagtgtaag catgttgaga
    12181 atattggttc ttgtgcctac tgttgaacga ggattgctct ggcgaataat attttgttga
    12241 acagctatag tgggagctaa accttgaata ttatcaaact tatcttcact atcaatacct
    12301 gcaagtatac ctaaagattg cagatattgt ttacgccctt cctcaaatac aatgtcaaac
    12361 ataaggctag acttacctga gccactgaca cctgttgcaa caacaagctt atcttttgga
    12421 atagaaacat ctatattttt aagattgtgt atctttgctc cattaatttt tatttctttc
    12481 attggttcac ctcattttat caattttatc aaaccgtaaa atagggatat attaatcctt
    12541 aatacaagaa tagattaggt gaggttatta aaaaggttct ataggtaaat atatatcgac
    12601 caagtgtcta ttttgagggt gattgtgtgg gttgttaaga tatacttcaa aagggaagga
    12661 ggcacgtagc ttataattac catttgatag ccattcatta tacataaaat cccacgcatc
    12721 actatattca gattttgaaa tatcaaaatg tcccacaaga tattttccag aaggtattat
    12781 catattgcca agattgtcat tctctttgac aacaatgtta tctggaatgg tcatgcaaat
    12841 actagtcctt aattgatgct cctgtgtaaa ttgtggatta tcatgataaa tggctaatat
    12901 ttgtgtgttt tcaagtccaa gtagattctg ctcacttgca tatgtgaata accgttctaa
    12961 aagttttggg aaagtactgg ttagattacc atatgaacca gtatatctag tgtaaatagt
    13021 attcatatta tcaagttcta gaatttcaat ttcaccttta acctctctag tattcttaca
    13081 tttaggtttt gatatgcttc tattgtactg agaaatttta tatggttctt tgcaattctt
    13141 gctataattg tttctatatt ttatagggct tattttatag taatttttaa atgctctcga
    13201 gaaaactgct gaatcagtaa agccgaagta gtgtgcaata tcagtaacag tcatgtcagt
    13261 tctatgaata agaaaagcag ttgcattttc tagttttagc ctatttacat attgtgccaa
    13321 agtttctttt tctattgctt tgaagattct atgaaaatga aattttgaaa aacctgctat
    13381 attagaaagt tcatcaagag aaagaggttc atgtaaatgt tcttctatat agtcttgcac
    13441 tttgcaaata cgatttagat attctttttt acttttagca tttgtcatat atagccctcc
    13501 ttattgtgta aaatgtgtat caattaattt atagcaatat agtaataaaa ttttatgttt
    13561 ttgtaattta tgataaataa gatagcgttt aattttttat aattgataaa agatataaga
    13621 tgccttatat ttgtctttgc atataagtat agtcttcgta ataattagta taccatagtg
    13681 atttattttt aattgtgtat ttatataaat acacaactta tgatatggac gaattattat
    13741 gcaaataagc tgtaattaaa taaattgcaa tataaaccag ttgaaacata agttagggaa
    13801 atattatc
//
LOCUS       DBGJFU010000099        13799 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.099, whole genome shotgun sequence.
ACCESSION   DBGJFU010000099 DBGJFU010000000
VERSION     DBGJFU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.099"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            183..323
                     /locus_tag="ACOIAS_002751"
     CDS             183..323
                     /locus_tag="ACOIAS_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702453.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(498..818)
                     /locus_tag="ACOIAS_002752"
     CDS             complement(498..818)
                     /locus_tag="ACOIAS_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HGZ2702454.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(938..1675)
                     /locus_tag="ACOIAS_002753"
     CDS             complement(938..1675)
                     /locus_tag="ACOIAS_002753"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HGZ2702455.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1890..3713
                     /gene="acd"
                     /locus_tag="ACOIAS_002754"
     CDS             1890..3713
                     /gene="acd"
                     /locus_tag="ACOIAS_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HGZ2702456.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3928..8226
                     /locus_tag="ACOIAS_002755"
     CDS             3928..8226
                     /locus_tag="ACOIAS_002755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGZ2702457.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8430..8891
                     /gene="rimP"
                     /locus_tag="ACOIAS_002756"
     CDS             8430..8891
                     /gene="rimP"
                     /locus_tag="ACOIAS_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGZ2702458.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8921..10075
                     /gene="nusA"
                     /locus_tag="ACOIAS_002757"
     CDS             8921..10075
                     /gene="nusA"
                     /locus_tag="ACOIAS_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGZ2702459.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10082..10363
                     /locus_tag="ACOIAS_002758"
     CDS             10082..10363
                     /locus_tag="ACOIAS_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HGZ2702460.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10353..10664
                     /locus_tag="ACOIAS_002759"
     CDS             10353..10664
                     /locus_tag="ACOIAS_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HGZ2702461.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10686..12626
                     /gene="infB"
                     /locus_tag="ACOIAS_002760"
     CDS             10686..12626
                     /gene="infB"
                     /locus_tag="ACOIAS_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGZ2702462.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12669..13049
                     /gene="rbfA"
                     /locus_tag="ACOIAS_002761"
     CDS             12669..13049
                     /gene="rbfA"
                     /locus_tag="ACOIAS_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGZ2702463.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13030..13707
                     /locus_tag="ACOIAS_002762"
                     /pseudo
     CDS             13030..13707
                     /locus_tag="ACOIAS_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
BASE COUNT         5506 a         1566 c         2590 g         4137 t
ORIGIN      
        1 atagttagat tttttatatg aattttaata ggtaaaatat cctattgttt tgtagatata
       61 tcctaattat gtcaaaaaat aaaaaaattt ttatttatta gagtttttgg aaataataat
      121 aacagatatt ataattaatt aacagttttt gtaagtattt gacaatagag agggtgtaac
      181 aaatgggaat aatagagtca gcatcaaaat tggctgagat ggttcattta ttggctgttg
      241 aaaaggggat aactgatata gaagcatggg atgaagctgt gaaagaatac agcaaaattt
      301 atgaagaaag aagaaatgaa tagaatgata aaaaatgaaa acttgatgat aaaaaataac
      361 tttatttaaa gaaattcaat ttttatagaa gtattaaaaa taatataaat aaaaagatgg
      421 tatttcaaat gtaagtaaaa aatcattttg ggataccatc cttattattt attttaataa
      481 tatataagta ataaatctta cttatccata ttttttatgt aacctctttt ttctaacttt
      541 tttatatgct ccttacgtat ttcatcaaga ttaataccat attgttcttc tagcttaaac
      601 atcatagtaa cacatgtctg cgctacatcc attagttcct tagcagtttt ctttataata
      661 tcaacttcat ctaaatcatt attttctcca cttagatttc tgaatttacc tatacattca
      721 gctaattcac cttgttcttc acccattttg ataaatgtag attctagtgt tgggtctaaa
      781 ttattaagct ctggtaaaga tatagttttt agtttcataa aagctccttt caagttattc
      841 gtatttatac taaaacatca taacaaaaat aatactaaaa aaaaacttta aattcaacat
      901 aaaaacttac atatataata aaaatcatga aataaatcta tatttgtctt aatacagcct
      961 catataaaac ttctccaata ggtttattta ttacaagaga agctgactta tcatagactg
     1021 tatttgcttt attaataaga gcaatataat cacctttgta ataatctata aaacttgctg
     1081 ctgggtacac agctaatgat gtacctccta taatcaataa atcagcatta cttatagcag
     1141 aaattgtttt agtaatgaca tcactgtcaa gagcttcctc ataaagtact acatctggtt
     1201 ttactataga accacagttg tcacagtaag gaatattacc tcccagattt aacatagatt
     1261 ctaagtcaaa aaacttacca catttagtac aataatttct atgtacagaa ccatgaagtt
     1321 ctagcacatt tttacttcca gccatttgat gaagtccatc aatattttga gttatgacag
     1381 ctttaagctt acccatttct tcgagttttg ctaaagctat atgagcatta ttgggttttg
     1441 cattaggata tattaattta tccttataaa attcaaaaaa ttcttctgga tatctaacaa
     1501 aaaaagtatg tgaaactaat tgttcagcag taaattgttt attgagtttt tggctaaata
     1561 atccagtaga acttctaaag tctggaatat tagattcagt tgaaacacct gctccaccaa
     1621 aaaatacaat attattgtga ttagctatta agtcttttaa gctatttgca tccatagtta
     1681 cacctccaaa ttattatata taaattgtac tacatattag gtcgtttatg ttttattaat
     1741 ttaaataaat atatagttta ataagttaaa taaagggaat ataaataaaa tgtatacaga
     1801 gtataaatag acaatattta ctaaaataac aagttgtcgt ataattatat taaaattgtt
     1861 ttagtgtata agaagggcgg gggaagtacg tgaagaaaaa agcagcttta gcaacattgg
     1921 ctatgttacc cttaggtgtg gtaaatgcac atgctgatgg agatataggt atagtgacta
     1981 taaattattt aaatgtaaga aatgaaccaa ctgccgaaag tagcatagcc tttgttgcta
     2041 aaaaagatga taaagttttg attaaagact cttctaatgg atggtataaa ataaaagctg
     2101 aatctggaca agaaggttgg gcttcatcaa aatatatagc aaaatcaaat agtgactctt
     2161 taagaacgtc tacaaataaa gaaaaacaag ttatttcaaa tagtttaaat atgagaaatg
     2221 gagcaggtac aagttacaga gtaataactg tactaaaaaa aggtcaaaaa gtagaagtaa
     2281 tatcagagag taatggatgg tctaaaatca aatatgatgg aagactagga tatgtatcta
     2341 gctcttattt aggagacgtt tcaaactcaa ctaataaatc aaaaactaaa caagtgaata
     2401 caacttcact aaatgtaagg agtggaccaa atacaagtta tggtttgtta ggcaagttgc
     2461 caaaaggaag taaagtagaa gtaatatcag aaagtaatgg atggtccaaa ataaagtata
     2521 atggaaaaga tgcctatgta tctagtatgt acttatcgga tgtaagtcaa agtaattcag
     2581 acaattctag tcaaagcaat gataaaaaga atactgataa ggttgtaaat acagcttctt
     2641 taaatgtaag aagtggacca ggttctacat atagtaagtt gggaaaagtt tataaaggaa
     2701 gtaaagtaac tgtactatca gaaagtagtg gatgggctaa gattaatttt aacaataaag
     2761 aagcatttgt agtaggcaat tatttatcta cctcagcaga tacttcaaat aataactcaa
     2821 atagtaactc tgataacagt tctaatagta atggtaataa ttcttcatca tcaggccaag
     2881 taaatggtat gtcaggtata agtggggcta aaattgatta taaatctttg agttatactt
     2941 tagaatctca tataagtaaa caagttgaga aagcagcatc aggaggaaat gtgatagctc
     3001 caagtaatag gaaaagtact ccaagtcctg agtttagtac tttttcagct caaagaacaa
     3061 gctcatttgt aaatgcaagt tcaagtgata tagaatatta tttaaatcct aagaatttta
     3121 caaatactac taaaggtatg atgcagtttt taaagattaa cagttataga gatggtattt
     3181 cagaatcaag tcttaactcg tatcttaatg gtttatcttc tagtgtattt aaaaaccagg
     3241 gagcagcctt tataaatgct gccaagaagt ataatataga tgttgtatat ctagtatctc
     3301 atgctatgtg ggaaactgct tatggtaagt ctacacttgc ccaaggtcaa acattaacat
     3361 cttataaggg acaacctctt agtaagccag ttaaagtata caattttttt ggtataggag
     3421 caatagataa aagtgctaat gtttcaggtg cagaagcagc gtattccaat ggctggacta
     3481 gtgtagaagc tacaatagat ggctctgcaa agtggatatc acaaaattac gtaaatagtt
     3541 ctaagtacaa tcagaatact atctacaaga tgaagtggaa ttatgattat acatggcatc
     3601 agtatgcaac tgatgtaaac tgggctaatg gaatttctgg aattatggaa aatttaattg
     3661 gtctttatgg aggaggaagt agtttggttt ttgaagttcc tcaatataag taatacataa
     3721 ataaaaaata tagactatgg gtagttttca ttttatttaa attatctata gtctttttgt
     3781 atataattta taatctgtaa atttaatgtt aacaaaactt ttatgttata atattaattc
     3841 taagtaaata taagtgagta ataggtagaa atagataaaa agtggtaata atatatataa
     3901 agataatttt gagaaaggag gatatatatg gaaagtatta aggaatattt ggataagtta
     3961 gaaattaata atagtggtct tggaaagcaa cttaaagaag tttatataaa tagagtagtt
     4021 tattttaagg aagataagat agtttatttt tatctaactt ctaaagatat tgtttctcat
     4081 gaacttttag ataaatttaa agaagaattg atgtataaac tcgattattt taaagatatg
     4141 aggatgaaga taagatttac tggtcttgaa agaaaatcca ataaagatgt tataaaaaaa
     4201 tattggaata atatattgta tatattaaag tatctttgtc catctattgc agggtggtat
     4261 aaacaagtag aatttctttg tttagaagaa gaattaaaaa taaaacttcc aaaaggtata
     4321 ttttatgagc gattaatgaa aaaaaatgta acttatgtac ttaaaaccgt gcttagcgaa
     4381 gagttgggat tagatttaaa tataacaata gaaaaagctg ttgatgaaaa ggtaaataaa
     4441 gaaagactca taagaatcaa tgatagagaa atggaagaaa aaattagggc tttagaaatt
     4501 ggaaaagtta ataactgtga agaaaatgaa gaggaaagct atgtcattaa gtctgatgta
     4561 gatgaaaatt taatctatgg agataatgca aatgctatga ttgaaaatat agttgaatta
     4621 aatgcatctt ctggaacagt tgcagttgtt ggagatatat ttgatgttga cactaaagaa
     4681 cttaaaaacg gaaaaatact tatgatagct tcaattacag attatactag ctcaataagc
     4741 tgtaaacttt ttttaacaga tacaaataag gatggagtac ttgaaaatgt taaaaaagga
     4801 gcatacttaa aaatcaaggg tgacatagtt tatgatacat atcaaagaga aattagtatg
     4861 atgattagtg ggataagaaa agaaactaga atagagagag aagataaatc agaggaaaaa
     4921 agagtagaac tccatgcaca tacacaaatg tcttctatgg atgctatttg ttctgttaaa
     4981 aagcttgtag agcgtgcagc caaatggggt cattctgcta ttgctataac agaccatggt
     5041 gttgtacaag gatttccaga tgctatgaat gcaggtaagt caaataatat aaaaatactt
     5101 tatggagtag aagggtacct ggttgaagat gattctctca ttatagagga tgcaaatgat
     5161 aaagaacttt cgcagacatt tgttgtattt gacatagaga caactggctt ttctaataca
     5221 aatgataaaa taactgagat tggtgctgta aaaatagaaa actttaagat tgtagataga
     5281 tttagtgagt taataaatcc tgaaaaagat atttcttata agatacagga attgacagga
     5341 ataactaatg aattaattaa agacaagcca accattgaag aagttcttcc taagtttatg
     5401 gaatttgttg gagatagtgt attagtagca cataatgcag agtttgatac tggatttata
     5461 tcgcaaaaat gtagggaaca aggcttgtta tataataaca aaaaagtaga tacacttatg
     5521 ttagcaagag taatgttgcc aaacttaaaa agacatagat taaatgtcgt tgcaaaagaa
     5581 cttggtattc ctcttttaaa tcaccataga gcagtggatg atgctgaagc tacagcactt
     5641 atatttaata agtttttgca gatgcttaca gaaaaaggtg caaaaactct aagtgatgta
     5701 aacaatatac ttggaaagat tgattacaca aagcttagca caaatcatat aaccttgata
     5761 gcaaaaaatt ctataggtat aaaaaattta tataaaataa tttcagatgc acatgtaaat
     5821 cattttttta gagcaccaag aatccttaga agtgtattaa atgaatataa agaaggatta
     5881 ataattggtt cggcttgtga agctggggtg gtttttcaag ctgtaaagaa aaacgtgtct
     5941 gatgaggaaa tgaaaaaaat aatagattta tacgattata ttgaagtaat gcctatagat
     6001 aataatagat ttatgattga taaaggagaa gtaaaggacg aagaagagtt aagggagttg
     6061 aataggaaac taattgatac agccaaaaaa tttgacaaaa ttccagtagc tactggggat
     6121 gttcattttt tagacaaaca tgaagctgtc ttaagaaaag ttcttaaata ttctcaaggt
     6181 tttaaagtag atgaagaaga aacttattta cattttagaa caacagatga aatgttggaa
     6241 gagtttagtt atcttggaga agaattggca tatgaagttg tagttgaaaa tagtaatctg
     6301 attgccgata tggttgaaga tataaaacct ataccagatg atacttatcc tccaattata
     6361 gagggctcag atgtggagtt aagagaaatg tgttacaaaa aagccaaacg aatctatgga
     6421 gacccaatac cagaaatagt tcaaaaaaga ctggatagag aattaaattc aataataagc
     6481 aatgggtatg cagttatgta tattatagct caaaaactgg ttgcaaaatc tctttcagat
     6541 ggatatttag ttggttcaag aggctcagtt ggctcatctt ttgcagctac tatgagtgat
     6601 ataacagaag ttaatcctct tccagcacat tatatttgtg aaaacgaaga ctgtaaatat
     6661 tcattctttt atgaaatagg tgaatggggc tcaggtgttg atttaccaga caaggattgt
     6721 ccaaagtgtg gaagaaaact tgctaaaaac ggtcatgata ttccatttga ggttttctta
     6781 ggttttgaag gtgataagga accagatata gacttaaatt tctcaggaaa ttatcagcca
     6841 acaattcata aatacacaga agaactattt ggagaggggt atgtatatag ggctgggact
     6901 ataggtactg tagcagaaaa aacagctttt ggatacgcaa gaaaatatgt ggaagaaaat
     6961 aatataagtg taccaaatgc agaagtactt aggctttcaa atggatgtac tggagtaaaa
     7021 agaacctcag gacaacatcc tggtggagtt atggttatac cagactataa agatgtatac
     7081 gattttacac ctatacagta tccagcaaat gatacttctt gtggtgttat aactactcac
     7141 tttgattatc attcaataag tggtagaata cttaaacttg atatacttgg acacgacggt
     7201 cctaccatta taaggatgct tgaagatatg actggaataa atataacaga aattccttta
     7261 gatgataaag agactatgtc tctatttaca tctacagaag cattaggtgt tacaccagaa
     7321 gaaataaact gtcctatagg ttgccttgct gtaccagagt ttggaactaa gtttgtacga
     7381 caaatgttac ttgacacaaa acctactaca tttgcagaac ttgtacgtat atctggtctc
     7441 tctcatggga cagatgtttg ggtaaataat gcacaagact tagttagaga agatatagtt
     7501 ggattaaaag atgttatatc tacacgagat gacataatga actatttaat atttaaagga
     7561 ttacctccca aaatgagctt tacaataatg gagagtgtaa gaaaaggtaa aggtttaaaa
     7621 ccagaacaca ttgaagaaat gaaaaaaaat aatgttccag aatggtatat aggctcatgt
     7681 aaaaagatta agtacatgtt cccaaaagca catgctgttg cttatgtaat gacatcattt
     7741 agaattgcat attgtaaagt tcactatcca gaagcatttt atgcgactta ttttacgaca
     7801 aaagttgagg attttgatgc agatttaatt gttaaaggat tagatgctat aaaatcaaaa
     7861 ataaatgaaa ttgagtcact gggaaatgat gcaaccacaa aagaaaaagg tatgttgacc
     7921 gttttagaag ttgctcttga aatgtatgct agaggaataa aattattacc agttgatata
     7981 tataaatcag atgcaactga gtttatagtt gtaggagaaa aaacattact tccaccaatg
     8041 gcagcaatac aaggtcttgg aggaaatgcg gctataaaca tccaaaatga aagaaaaaat
     8101 ggtgaattta tatcaaaaga agatttgaga aagagaacaa aaatatcaaa aacggtaata
     8161 gaaactctta ctaatcatgg ttcacttgag aatatgagtg agagaaatca attgtcattg
     8221 ttttaagtta gcccaaaaag ttgcaaataa aagaatttta tgatatcata gtatagaaaa
     8281 tagtatactt tgcagaaact aagagtgaga cagctgttct cactctttta ttttaaatat
     8341 attattaaaa agctggttta tttttattaa atatatttta tttaagatta tataaactgg
     8401 tcatttacaa ttgaatagga ggaactttaa tgaaaaaaaa tttagaagct acaattgagg
     8461 agattgtaac taaaataacg gatgaacatg gatttgaaat ggttgatgtt gaatatgtaa
     8521 aagaagcagg agaatactac ttaagagtat acatagataa agaagaagga atatctttaa
     8581 atgaatgtga attagttagt agagaattaa gtccaatact agatgaaaaa gaccctataa
     8641 aagaaaatta ctttttagag gtatcttctc cagggcttga tagagcatta aaaaaagaca
     8701 gagattttgt tagatatcaa ggtagagatg tagatttaaa attatataaa ccattaaatg
     8761 gatgtaagca atttgaaggt gaacttgtag gattaacaga agataataat ataaaaatca
     8821 ttgcaaatgg taaggaaatg gaatttaata gaaaagatgt tgctattgtc agattagcaa
     8881 taaagtttta gtggtatatt aaatagggag gatataaaaa atgaatcatg aatttatgga
     8941 agcattagat gaattagtag ttgacagagg tatagataaa gaaatactta tagatactat
     9001 agaacaagct cttttaactg catacaagaa aaactttggt tcagcacaaa atgttagagt
     9061 tgaatttgat agagacaagg gagatataaa agtattttct caaagagttg ttgttgatga
     9121 atcagattta tatgatactt tcttagaaat agagttagca gatgctagag agataaatcc
     9181 taattatgaa ttaggtgata ttatagaaca tgaagttaca cctaaaaact ttggaagaat
     9241 tgcagctcaa acagcaaagc aagtagttgt tcaaagaata agggaagctg agagagaaat
     9301 agtgtataat gaatttatag aaaaagaaaa tgaaatagtt acaggagagg ttgctagagc
     9361 aaataaaaat gttgtttatg taaatcttgg aagaatagaa ggtataatga ctcaaagtga
     9421 acaaatacca tttgagaatt ataaagcagg tcaaaaaata aaattctata tacttgaagt
     9481 taaaaagact aacaaaggac ctcaaatagt tgtatcaaga agtcatccgg gtctagtaaa
     9541 gagattgttt gaagaagaag ttcctgaaat atatgaagga atagttcaaa taaaatctgt
     9601 agcaagagaa gcagggtcaa gaactaaaat ggcagtaaga tctatagatg aaaaaataga
     9661 cccaattgga gcttgtgttg gaccaaaagg acttagagta aaaaatatag tggatgaact
     9721 tggcgatgag aaaatagata taataaaata tagtgaagac ccagcagagt ttatatcagc
     9781 agcacttagc ccatcaaagg ttgttagagt agatatagat gaagatgaaa agtctgcctt
     9841 agtaatcgtt ccagattatc agttatcgct tgctataggt aaagaaggtc aaaatgcaag
     9901 actagcagca aaattgacta actggaaaat agatattaag agcgaatctc aagcagaaca
     9961 agaaatgtta aatcttgaaa attccaaaga aatagaagct gaggatgttg aagctgaatt
    10021 tatcgaccaa gaagagttag atacttcaga ggataatttt agtgaagaag agtaaggagg
    10081 gatgattttg aaaaaagtaa aaaaagtgcc tcaaagaaaa tgcatagcat gtcaagatag
    10141 agactctaaa aaagagctta taagaatagt taaaaataaa gaaggaaaaa tatttttaga
    10201 cccaacagga agagccaatg gaagaggagc atatgtatgt aaaagctctg aatgtctaaa
    10261 aaaagctata aagagtaaag cattaaataa agcttttaaa atagatgttc ctgatgaagt
    10321 ctatgataat ctacttatgg agttagaaga atatgaaaaa taataaagaa aaagtacttt
    10381 cgttcttagg gctcgcaact cgtgcaggga aaatagtgtc tggtgatgat tcaactttgc
    10441 ttgaccttaa aaaaggaaaa gtaaatttga ttttaatcgc agaagatgca tcaaataata
    10501 ctaaaaaact ttttaaagat aaatcaactt ttagaaatat tccatatcta tttttttcaa
    10561 caaaagagga aataggattt gcaataggaa aatctccaag agcagttgta ggaataaaag
    10621 atgaaaattt ttctaaaaag ataattgaac taatagaaat ttaaaataaa atagggggtg
    10681 atcttgtgtc aaaaacaaga gtataccaaa tagcagaaga attaaatata tcaaatgaag
    10741 agcttataaa taagttagct gaattagata taaatgtaac tgacaaagat tcagtattag
    10801 aaggtgaaga gttagaatta gcacttgaaa tgttaggtga agatttatct caagaaaatg
    10861 gaaatgttat agaaatagat ggaaagttga cagtacaagt attagcaacg aaattagata
    10921 aatctccatc agaaataatt atgaagctta tgaaaatggg aactatggca acaataaatc
    10981 aagagataag ttttgagatt gctgctttgg ctgccaaaga ctatggattt gaattaacag
    11041 tagctgaaag tgatgataca gaagctttag aaatagaagc tttaatggaa attgaagaag
    11101 acaaagaaga ggatttaaaa cctagaccac cagttgttac agttatggga catgttgacc
    11161 atggtaagac atctttacta gatgcaataa gaaaaactga tgtgatctca ggtgaagctg
    11221 gaggaataac tcagcatata ggtgcttcag aagttaaaat caatggacac aaaatagtat
    11281 ttttagatac tccaggacat gaggctttca catcaatgag agcaagagga gcacaagtta
    11341 cagatatagc aatattagta gttgcagcag atgatggtat aatgcctcaa acagttgaag
    11401 ctataaacca tgctaaagca gcaggtgtac ctttaatagt tgctattaat aaaatagata
    11461 aaccaggtgc aaacccagat aaggttaaac aggaattagc agaccaagga ttattagttg
    11521 aagactgggg tggagaagta atagcagttc cagtatcagc taagaaaaaa gaaggaatag
    11581 atacactttt agaaatggta ttattggttg ctgaaatgga agagttaaga gctaatccaa
    11641 ataaaagagc agtaggaact gttatagaag ctgagcttga taaaggaaga gggccagttg
    11701 caacagtact tgttcaaggt ggtactttaa cagtaggtga cccaatcgtt gcaggtgttg
    11761 catgtggtaa agttagagca atgataaatg caaaaggtaa gagagtaaaa acagcaggac
    11821 catctacagc agtcgagata ttaggtctat cagaagttcc acaaggagga gaccaatttg
    11881 tcgaagttcc aactgataag attgctagaa gtgtagcagc aagaagacaa caaatagtta
    11941 gagatgaaat gttaaaatca actcaaagat tatcactaga tgctctattt agtcaaatga
    12001 gtgaaggttc tataaaagac cttaatatag ttataaaagc agatgtacaa ggttcagtac
    12061 aggcagttaa acaatcttta gaaaaattat caaatgaaga agttcaagtt aaagtaatac
    12121 atggtggtgt tggagctgta acagaatcag acatattact tgcagcagct tctaacgcaa
    12181 taataatagg attcaatgta agaccagttc caggtgcaga atctctagga gaaaaagaaa
    12241 atgttgatat aagaacatat actataatat acaaggcaat agaagatata caagcggcta
    12301 tgactggtat gttagaccct gaatatgttg atgaagaaac aggaaaagct gaaataagag
    12361 aaatatataa aatatctggt gttggaacag tagctggatg ttatgtgact aatggtaaga
    12421 tatttagaaa ttgtaaagta agattagtta gagatagcat aataatacat gaaggtgagc
    12481 ttgcagcact taagagattt aaagatgatg taaaagaagt taattctgga tacgaatgtg
    12541 gtatgagttt tgtaaattat aacgacataa aagaaggcga tatagtagaa gcttatataa
    12601 ctaaagaagt agaaagaaaa ttataaattc gtatcaaaga aaatcagtta gtaaagaagg
    12661 tcgatggtat ggcatcatat aatagaacaa gaaggatagc agaagaaata agaaaagtcg
    12721 taagtactat gcttataaat ggagtaaaag accctagaat aacatctatg gtgagtgtaa
    12781 ctgatgtaga agttacaaat gacctaagat atgcttatgt atatgtaagt attctaggcg
    12841 gagatgaaga atccacacta acaggtctta aaagtgctgg aggatatata agaagagaag
    12901 taggaaaaaa tataaaactt agatatattc ctgagatagt atttaaactt gatgattcaa
    12961 tagaaaaagg aatgtatatg gatagcctta taaaaagagt taatgaaaaa aatgctcaac
    13021 aaaatgagga tgaaaattat gatgaataat atggatagta ttataaacta tatttatgag
    13081 ggtgactatt ttatagttac ctctcatatc agtccagatg gagacaatat tggttctaca
    13141 ctttcaatgt attatacatt gaagaattta ggaaaaaatg tatattatgt acttgatgat
    13201 gaagctccat taaatttaag gtttttggtt gaaggtgtaa aaatctataa gtctaatgaa
    13261 tttaatatga agaactattc tctaattgca cttgattgtg gggataagat gagaatatgt
    13321 gtgagtgatg aaatcaaaaa taatgcatca aaattaatct gtattgacca tcatgctagt
    13381 aatgattcat atggagattt aaattatgta gatattgatg catcttctac atgtgaatta
    13441 gtttataatt tattagttag atttcagcaa ataaaagata ttaatattat aaatgaagat
    13501 atagcaacat gtttatatac aggtcttgta actgatactg gaaacttttc gtattcaaat
    13561 gtacatgcaa gtagctttga aatggcaaaa aatttacttg ttttaggtgc acaaaaaaat
    13621 actataatac aaaatatata tcaaagcaat tcttctggtt attacaaatt attaggagaa
    13681 gctctaaagg gtttggagat atttgattaa aaaatagtta cgcacactcg tgactttagt
    13741 catgagttag tagctaaaat agtcagcata tagggaaact tatatgtaga gataggata
//
LOCUS       DBGJFU010000100        13712 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.100, whole genome shotgun sequence.
ACCESSION   DBGJFU010000100 DBGJFU010000000
VERSION     DBGJFU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.100"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(225..1892)
                     /locus_tag="ACOIAS_002763"
     CDS             complement(225..1892)
                     /locus_tag="ACOIAS_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HGZ2702464.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(1966..2721)
                     /locus_tag="ACOIAS_002764"
     CDS             complement(1966..2721)
                     /locus_tag="ACOIAS_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGZ2702465.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(2731..3267)
                     /gene="lepB"
                     /locus_tag="ACOIAS_002765"
     CDS             complement(2731..3267)
                     /gene="lepB"
                     /locus_tag="ACOIAS_002765"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGZ2702466.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3433..3969)
                     /gene="lepB"
                     /locus_tag="ACOIAS_002766"
     CDS             complement(3433..3969)
                     /gene="lepB"
                     /locus_tag="ACOIAS_002766"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGZ2702467.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4069..5379)
                     /locus_tag="ACOIAS_002767"
     CDS             complement(4069..5379)
                     /locus_tag="ACOIAS_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HGZ2702468.1"
                     /translation="MTELPNEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(5458..6174)
                     /locus_tag="ACOIAS_002768"
     CDS             complement(5458..6174)
                     /locus_tag="ACOIAS_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HGZ2702469.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            6419..7690
                     /gene="sleC"
                     /locus_tag="ACOIAS_002769"
     CDS             6419..7690
                     /gene="sleC"
                     /locus_tag="ACOIAS_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic germination protein SleC"
                     /protein_id="HGZ2702470.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8198..8854)
                     /locus_tag="ACOIAS_002770"
     CDS             complement(8198..8854)
                     /locus_tag="ACOIAS_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702471.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(10046..10456)
                     /locus_tag="ACOIAS_002771"
     CDS             complement(10046..10456)
                     /locus_tag="ACOIAS_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702472.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(10672..12054)
                     /locus_tag="ACOIAS_002772"
     CDS             complement(10672..12054)
                     /locus_tag="ACOIAS_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2702473.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNGKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(12065..12424)
                     /locus_tag="ACOIAS_002773"
     CDS             complement(12065..12424)
                     /locus_tag="ACOIAS_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2702474.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(12629..13408)
                     /locus_tag="ACOIAS_002774"
     CDS             complement(12629..13408)
                     /locus_tag="ACOIAS_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HGZ2702475.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4686 a         2465 c         1340 g         5221 t
ORIGIN      
        1 aaaataatca cataaactaa caaaatatta aacaaaaaag taaaatttaa aataaaaagc
       61 ttgcttcata caaaagtata aaacaagcct ttttatatat caactcaaac tttaaaatct
      121 gtatcaattt aattctccac tttacactta atatacttaa cataaaccaa taacactact
      181 tgctatgcca tttaatcttc ataaaacaat ctataataca cacattaatc aactatttta
      241 cttcctccaa taaattccct aagaattgaa atacctggta tgtcagaagt atcgtccaat
      301 tcaacaaaat actgcaaaaa ctttagtaat ctagtagctt caatatgtgc aggattatct
      361 tcctttatct cttccaacaa aggtttcgca aatcgtttta aaactgccaa ttcatataca
      421 caagcagagt taaatatagc atctgcctcc tcttggtacg ggaatatatt cttcttttct
      481 cctcttctaa ctgatgccca ttgtaaaata gtattatcag cactatatcc tctaaaatta
      541 tagtctctaa caattcttct tatcattctc aaatcagcag tagggattcg attatggtca
      601 tccaaattta tttgagtaag ggcacttatg tatatcttaa atttatcttc atctggtata
      661 gatgatgtta aaataggatt tagcccatgt ataccttcta atataattgg ctgatttgaa
      721 tctatcttta tctttttacc agtgtattct ctcttacctt ctttaaaatt gaatcttata
      781 tcatcaattt cttcaccagc taaaagtttc tttaagtcta agttaaactt ctctaaatct
      841 attgcataaa ttgattcaaa atcataattt ccatattcat ctaaaggagt atgttttcta
      901 tctataaaat aatcatctaa agaaattgaa ataggattta aattatttac tctaagatgt
      961 attgataatc tgtgagcaaa acttgttttt ccagatgaag atggagctgc tattaaaact
     1021 actctcttat tgccatcctt tatcatatcc gctatttgag atagcttctt ttcatgcaat
     1081 gcttcaaatg tccttattat atctccatat tcattgtttt ctattatttt attaagagat
     1141 actaccttgt caattcccat aagtcttgac cattcttctg cttcataata tacacttgat
     1201 agcttaggtt gaggtacaaa tttcatagga agagtcttat cttcttcact aggtcctaaa
     1261 attataacgc ctccattata aagcttaata tcaaatgatt ttatataacc agttgatggc
     1321 aacatataac cataaaaatg gtctatatag tttctacact tatatacttt agcactatca
     1381 tactctttgt atttcaaaag ctctgccttt tcatacattt cattattttc aaagatagaa
     1441 atagcttcat ttcttgtaac ttcaattttt tctataacat aatcgttgtt tactatctct
     1501 ttcatcttgt tttttatatt ttcaacatct gcttctttaa gctttctatc tatatgaact
     1561 tcacaatata gcccatctga tagtgaatgt tctattgtaa ctctgctatc ccaaaagatt
     1621 tctctacatg ccattacaaa tataaatgac atgactctaa agtaaactct ctctccatct
     1681 tcattagtag tatctaaaaa atttatctca caatccttct taactctaca atttagctct
     1741 tttagtttat tgtctacaac tgcaagtgtt atataaccct tatgctcatc acaaaattct
     1801 tttgctatat ctcccaatct gattccaggg gaattttctt ctaatgaata ttccttatct
     1861 tttccttcta tttttaagtt tatttttttc acttcaacat ctccttcctt ttgatgaata
     1921 taaattttaa ttaatgaggc atatcaacaa gataaagaga ggtatttaac cctctcctac
     1981 aatattattc aatttactta tagtatcttc aatgtcttcg taatttattt ctaatactaa
     2041 atattctgca tctaatgtta agacctcttt aatcccctct tcagataaaa ttcctcttcc
     2101 tataccaaca tgtgtatcat tgataccatc attatcactt aagtgtatca gtcttatttt
     2161 atcctttaag ttctttacat aagcatcctc atcaattaaa cagtgtccag tatcgtaaca
     2221 aaaacaaatc ttagaatttt taatcctctt aaatatatat tcaaaatcat aagatatatt
     2281 tccaatattt gaaaaatccc caccttttga ataagtattc tctatacata catatgaatt
     2341 tgtatttaat ttatctaaaa aatccacact atttcctaaa tatttgtctc tattctttct
     2401 aactctattt gtcataacat atcccaaatg aaggttaaaa taccttaaat caaatccttt
     2461 tagttcaaat tctatcttct caataaaatt tatccatgaa tttcgtatat actctatgtt
     2521 ttcgcaagta tttaattcca taggcaagtg tatccctata gataatccta acttagaaat
     2581 tctctcttta tatttacata actcactaca ttcagatata ttgtcaattc caatctctaa
     2641 atgagttatt tgctttatac tctcacaaat gtttaaagcc tcctcaatat taaacaataa
     2701 ggacgctata ccaactgcca tacaaatcac ctactcaaaa atacctatat ctgtaaaagg
     2761 aaatactctt aaaataactt ttcctttaat attttcttca tctactaatc ccacttcttt
     2821 atatctactg tctaaactaa cttctctatt gtctcccatc gcaaatactt ttcccttagg
     2881 aatcaccata tcaatatcgc cttcagtata attatcatgt atatatgaaa cttcatttaa
     2941 taacttacca tttatataaa ccttagaatc cttaattttt agatggtcac caggaacacc
     3001 tataaccctt tttacaagct cttttttcct tccattctct tgtaataaat cagtcttaaa
     3061 tatcattata tctccacatt ttggttctga taacttatat gtcatcctgt ttgctaccaa
     3121 ataatcatgt gatttaagtg ttgggtacat tgattctcca tttactaatg ttggttttat
     3181 aaaacgagtt ataataaatg caaaaaaaag tgcagtaata attattttta tccactctac
     3241 aatctcttct ttaatagttt cattcattca caaattctcc tttatagtta tattatcttt
     3301 attgataatg tctcttaaac actatctttt agataagtct actaaataag gtgttgtctt
     3361 ttttatatag acaactattg tgtatggtct ggtgatttca gctaatcagc gattgctcca
     3421 aattttatat gtttagtcaa tagttcctat cttagaaaat ggatacaatc tgactagaac
     3481 ctttcctaaa atggtatgct cgtcgaccaa tccaacctca tcgtatctac tatctaaact
     3541 tttttctcta ttatctccca ttgcaaataa ttttccttct ggaactacga tatcaatatc
     3601 tccatcagta cgattattat gtatatagga agtctcatct aacaacttat catttacata
     3661 taccttggag tcttgtattt ttacatggtc accaggaaca cctataactc tttttactaa
     3721 atcttttttc tttccatttt cctctgttaa gtcggttttg aatactatta tatctttgta
     3781 ttttggctct cctaccttgt acgcaattct gttaactatc aaataatcac gttcaactaa
     3841 tgtaggatac attgattctc ctttgactat tgttggcact ataaaacgag ttattgcaaa
     3901 tgccaaaaca agagctatga caatcacttt tatccattct acaacttctt ttttaactgc
     3961 ttcacccact aaaatcgtct cctctgtgtt aaatattaag tcaatctaca gcaaaataat
     4021 ctaccataaa cctacaatat aaatattata cttctttttt ctaatagttt aaaatacttt
     4081 tctaagacct ttaggataat gattcttaat tacaccagaa gattcttttc cccaacctaa
     4141 aggtattcct tcaaccgaaa ccaaaaccca tcctctacta tcattagaat ttacaacatc
     4201 cccttttaaa tatttaagaa ttgattcact ctgtacactg aagttttcaa cattttttac
     4261 atcttcaact tttaagtaat gagatagtgc atgagaaggc tcaaatctat tcttctttaa
     4321 aacccctaaa tgcaaaccat atctcaatac ttttagtttt ttcgtatctg gatatatttc
     4381 aggaagtagg tacaaatttt ctcctcttaa ataaaattta ttttttttat tactataaaa
     4441 atcaaccttt aaaaatttct tttcaaactc cctataatcc tttatttctt tatctaaatt
     4501 ttttattttt aattctttga cattacattc ttcttcttct aattttttta ttttggcaac
     4561 aaaatgacct tctcccttta tcttatgagg ccataatctt tccatttcaa taagttctgc
     4621 atgtggatat tcactgataa actcatttat aacatgctca ttttcctccc ttgaaaaagt
     4681 acaagttgag taaattaaaa tgccatcctt ctttaacatt ttatatccat ctcttataat
     4741 ctctttttgg atggattgac attctactac ctttgcataa ctccaatctg atatggcaac
     4801 ctcatccttc ctaaacatac cttgcccaga acaaggtgca tctataataa ccttatcaaa
     4861 atacccatta aacgtctttc ttaagctatt agaatcggtg tttgttataa tacaatttct
     4921 cgctccaaat cgctctaaat tttcccctag agccttaatt ctactagcat taatttcatt
     4981 agaaaccaat aaacctgtat cgtttagctt agatagtata tatgtagatt ttccaccagg
     5041 agcagcacac atatcgagaa ctttttcacc ctctttaata tctgcctttg gtattacact
     5101 cattgcagaa ggctcttgca aatagtatgc tccagcctca tgcagtggat ttctaccagg
     5161 tctgtcaatt ttatcatcat aataaaaacc ttcttctgcc catggtattt gattcaaatc
     5221 aaacaactcc aaatcaaata attttttctt gtcaattttt aaagtattta ttctaagccc
     5281 agtagtctta ctttcctcat aactttttat aaaatcatca tattcatctt ttagtatttc
     5341 ttcaatatct tctaaaaatt catttggtaa ttctgtcaaa tctataatcc cccttcaatc
     5401 tgctttactt gtgtgtcatt tattatactt taattattta tgtctttcct ttaaatttta
     5461 tttcttcttc tttttcctaa gattaccgaa aaacactttt ttagagtgat tcatatattt
     5521 ctcaagttta tcaagattta tcttccacaa aattattaca taaactataa gtaatataat
     5581 agcccatcta tattctatca tcaatatgaa atcaaaaact ccatgtaata aaattggaat
     5641 taacactgcc ataaacaaat actcacgtct tttattttta tttccctcaa atttatactt
     5701 agaaatatag tagcccatag taattgcaaa cattacatga gctggtatag agataactgc
     5761 cctattaata ccaacttgca gtgctagatt tctactttca gaaaaaatat aaaccatatt
     5821 ttcaattgtt gcaaatccta aagctaaaaa tacagagtaa ataataccat ctaacttttc
     5881 agtaaagtgt ttttctttaa ttagggcagg aatcagtatc agagctttta caccttcttc
     5941 tacaagtcct gcaactacaa atgatacata tattagttct gaataccctt caaatacatt
     6001 aaacttcatc agtaaatttt ctaaaagtat aattataaca cttagaaaag cacctattaa
     6061 aaaaaattta attagatgga ttaggggttc tctacaatat ctatctttag accttatcca
     6121 aaacattcct atcaatatag gaactattgc taataaaaat aaatctagct tcatagatta
     6181 ttttcctttc aaaatttttg atttatttat gatttatatc atctacttat ttttaactta
     6241 ttcgtgtttt aaatacatac taaaatatac attaaattat tttaaattat ttaaatacaa
     6301 atatactcaa atactcaaaa aaatttaaat ttatatctat attatttaac aaattttaag
     6361 ctatattcat atcttgaaat ataagggaac taaattcatt taaagaaagg gtgatttgat
     6421 gcaagatggt ttcttaacag taagcataat tgatgctact aacaatagac ctattcaaaa
     6481 tgcagtagta aacatatatt caatgtctaa tggtagccaa agctcatcaa ctttatacca
     6541 aaacctaaga agtaatgaat ctggtcaagt gactggttta gtattaccag caccagatgt
     6601 agattactct ctacaaccat ctgatgttag accttatagc cagtatatag tggaagccat
     6661 agcagatgga tatgagacag ttgtcataga aggaacacag ttgttagcta caattgaagc
     6721 aagacaagga gttcccatgt ctcctagaac aagaagtaag cgttctttct caagacaaag
     6781 tgagttgatt tttgacatag gagaacatac tctatatggt acttatcctc ctaagattcc
     6841 agaaagtaac ttaaaaccat taccacctcc tactggtttc gttgtattag ataaccctgt
     6901 agtgcctgaa tttatagtag ttcatgatgg tttgccagaa gattcatcag ctccaaacta
     6961 ttggattcca tttaaagagt acattaaaaa catagcatca tctgaaatat attcaacttg
     7021 gcctgaacag acaatttatg caaatgtaat agctataata tcatttactt taaatagagt
     7081 ctttactgaa tggtatagaa ataaaggata taactttaca ataacttcaa caacagcata
     7141 cgaccataag tttataaata atagaaactt atttgaacca attaacgtag ttgtagatgc
     7201 tatttttaat acttttataa aaagacctcc tacatcaaga cagccactat tagcacaata
     7261 ttgtgatgga caaaaatccc aatgtcctga ccaaatgact caatggggca gtaaagactt
     7321 aggtgaccaa ggatatgact atgagagtat actaagatat ttctatggag atgagatagt
     7381 atttgaaaga gcgcctatag taagtggtgt accagtgtca ttccctggta ctacactaca
     7441 agttggttct tctggacagt atgttagaac catacaaaat cagttaaatg ctatatctaa
     7501 ttcttatcca gctgtaccaa aagttataga agatggtatc tatggaacag atacagaaaa
     7561 cgctgttaag atattccaag gaatctttgg tttacctcaa agcggagtag tagattttaa
     7621 aacatggtat gaaatatcca gagtttatgt agctacaact agaatagctt ctttaaatcc
     7681 tttaatttaa agcttgattt agaacataaa ctcaaattat tgaataaaat tattaattta
     7741 aaataaaaat ggctgttatg aactaatata taccataagt attacttata agtattgtag
     7801 tcttaacagc cattttttag ttataacttg tttttttaga atcttaaaac tttaaatggg
     7861 ggattttaag cttcttaaaa acagtgctta gcaacttaga tttctttaaa atattatatt
     7921 tatttttaag tttaataaaa tgctattcta acgaaaaacc tactttttat ttaaaatctc
     7981 acttattatt taaaatgcta cttctcactt agagtattac tccttattat ttaaaacact
     8041 atctatcact tagagtatta cttcttatta tttaaaacac tatccatcac ttagaatatt
     8101 acttcttata taaattatta tttattataa atacaaataa tattaattta aaaagttatt
     8161 cctaatttaa ataatactac tatcttcatc agtgaaccta aactgcaact ttagacttat
     8221 tagctaaagc caatattaga cctaaagcta tagatgcaaa tgcagaagca attactctat
     8281 actcagcagg gaataagaat acaattgtat gacatggtat ccagaaaaat ggtatagttt
     8341 ttaaaacact aaaaccaagg taagcattcc agtccacaat atttaccaca tctttaagtt
     8401 ttaccttaat cccctttcca acctcatatt ttttatctat aaaacagtcc ataaccttat
     8461 ggaataaaaa tacagttggt gacattagta tattcatagt tgcactctta aatagagcat
     8521 ttgcaaaagc tattccctca aatggaagca taccatccac ttgtgcttga cctacacctg
     8581 atgagtagat tgacattcca tatgtggtaa ctacacctat tattccccaa actattgctc
     8641 tataaaatac tcctactggt attttccaat ccttatacaa aattctagca ccaagtaaat
     8701 ctcccattgt agctaataca gcaaacttta aaaaacctcc aatatatgga tgtgcacctg
     8761 ttgcttccat aaatacttct ctactacttg gtattgctaa gacagcaata atacaaataa
     8821 gaactccact ccatataaaa tcgccttttt tcatatttat aaatactatt taatatataa
     8881 atagttctcc tttctattaa agatttattt aatttacaat ctatctacaa ttatttatta
     8941 atcacttatt attagatatt cattttccat atttaaattt attctatccc tttatctatt
     9001 tattttctta ctactctatc caccattatc tatatattta ttttacgatt actctttcac
     9061 cctaaccctt taattatcta ttttttctga tattcttatc taccatcatc catatattta
     9121 ttttacgatt actctttcac cctaaccctt taactatcta ttttttctta ctattcttat
     9181 ccacatcatc catatattta ttttacgatt actctttcac tctaaccctt taactatcta
     9241 ttttttctga tattcttatc caccatcacc catatattta ttttacaatt actcttttac
     9301 cctaaccctt taactatcta tttttttctg gtattcttat ctactattat ctgtatattt
     9361 attttatgat tactctttta acctaccccc tatactttat agtaatatgt ttttaacctt
     9421 tttcattatt cccaatatgc tttataacta caaatactct ccccccttaa aaataataat
     9481 aaaattaatc aataaattta taatcatatt attactctat gtattaagca attactatgc
     9541 caataaactt tgattttttt aaacaaatct taaaaatcct aaaatgctaa ttttctttaa
     9601 cttttaatga atctaataga taacatttaa ttttcgttat atttttcaga atatttctat
     9661 tattattttt aagaaaaaaa aataatgtta acatttctta acatacatta aaaaatgtta
     9721 acattataaa ttgggtttaa tacttttact ttgactcgat ttacttttat tctacttctt
     9781 actttatttt taacttagac tttatttttt tattacttat ttgagtctta tttattttat
     9841 tatcactcca tatgttcaat tacatttgtc taaattcttt gatactttta ttacataatt
     9901 ttaacaagct taagtgttat ttgggtagtg cagttgataa ttatttaaat aatcatcaac
     9961 tgcatgttta tatcattaca gtagcttaat gtatttaatc atacttactt aggattttga
    10021 aatatcttag agattatatt taacattagt caattgctgc ataattgtaa gcaattgaaa
    10081 tttgagaccc atcttcattt tctatagtga taggcttcat aatttttaat ccttcagttt
    10141 ttatcttttc taattcttgt tccattttct taattgtttc ctcaaggtct ttttgactta
    10201 catctttgac tgcattaggg tctttagcat agtcttttaa ttcttgaata tgtttagcat
    10261 actcttcata agtataatgc tcaacaccat acttttccaa ttcagtaggg tcagttattc
    10321 ctgattttat tagagattca agtttaggta aagcaatatt agatgaatca tttccatttg
    10381 caaatactgc tccagttcct cctagtacca ttgcagatac catcattgca cttataaggg
    10441 atttttttat tttcataacc aatcttcctt tcttagagat ttatttgggt agtgtagttt
    10501 gaaatgacta ttgcaatagt tatcaaccac atccttagac tatcacagta gtcatgttta
    10561 tgtcaagcct ttttaaaaaa cttttcaata aaaaatacta tctcaaaaca aaattaatta
    10621 ttttgagata gtattttaat tctaaatctt ttatattact aagatttatg gttactctat
    10681 ccatgttcta tattgttctt tattaactgc taacaaattc ccttttcttg atatgacaaa
    10741 gcgattatca ttatagccac tttgctcaat tgaaacttct cctgaatcag tataaagtac
    10801 atccttacca ttactagatt tatttacata cgtttcttcg ctattctcag aattagttac
    10861 tttgctcata gatgattctt catgctctct aaattcaatt acagatactt ttgccgtact
    10921 aaaatgagtc tctccatcac ttttagcttt actaacaaga atatcagatt ctactttggg
    10981 agaatataaa tcagttgatt tactacttat tgaaaatcca attttatcac tttgttcatt
    11041 ttttgttaaa ccaaaagatt caacctcatt aatctcttta ttttctgttg caaaaacaag
    11101 acctgtccca caaactaata cagttgaaac taaaaaagta acataagttg attttttaaa
    11161 tttcataata ttaactattc tcttttctat ttttgtcttt ccaaatccac tacatattgt
    11221 cattgaaaat ggattattct cctctgctag acttattagt gtggttgcat aatcagccct
    11281 atattcttca ccaagtatac tcataacctt ttcatcacaa ctaaattcta tgtctttatt
    11341 tagtaaaaaa aacatcaccc aaactaaggg attaaaccaa tggacaacaa gagctacaag
    11401 cataaataac tttaacagat tatcaaagct attaatatga accatttcat gagttaaaac
    11461 aaagtctaaa gtttgattat tagcataatt catttgcttt ggcaaaataa ttcgtggttt
    11521 taaaatacca gatgtaagtg gtgtcctagt aaattcagat atgcaaacaa taaatcttct
    11581 aaaacggcta ttcttacaca tccatttttt aatgaactca tgatttttta tatgaattga
    11641 agttttaaaa agattaatta ttttaaaata tctaaagcat aaaagtatgc taaacataat
    11701 agtaactata aaagacaaaa tgcccaacat ttccaacatc ataatatttt caatattaga
    11761 cattatttca aattcgtcag aattgttttt acctgtagag tcactagctt tcttaagagt
    11821 ataattttct gttgatgtat tacccaattc cacatcaaaa gtcgattcaa ttatactata
    11881 aatactaaaa ccagaattta aacttactgg tattagaagt ttaaataaaa caatcaacca
    11941 caataataca aatacctttt taggtaattt tttaaataaa catgctctta taaaaataac
    12001 tattaaaagt aatactccag aagtaaaact catttctaac aatttaatga acataaatct
    12061 tacctcactt taatttttca acaatttgtt taagttttgt aatctcatca cttgaaagat
    12121 tttctttatt aataaaagct gaaagaaatt tttctgaaga ccctccaaac attttattaa
    12181 ttaaagtttc tgtttcctgt ttttgtactt cctctttgct tattaaagct ttacaaacaa
    12241 aattcggctc aattctctct attgcacctt ttgacactaa tttttttata acggtatacg
    12301 tagtgtttct attccatcct gtttcttctt ttaaaatttt tactaactct ccagccttgc
    12361 tttctcctct ctcccataga acttccatta ctttaagttc tgaatcaaat aattttatct
    12421 ccatataaaa tcgctcctca aatatagtta tcacaatagt cattactata ataaaaaact
    12481 atcatagtag tctatactat gtcaatcatt tattttttaa taataaaaac tgtcttttta
    12541 aaaaattctt ataaaagaca gttttatcaa tgttatactt taatctttaa ttaaagccat
    12601 attttattta aatatataac tatatatctt agtacatagt ataagatttt tctttatcta
    12661 atagtatatc ctctacatct gggttttctt ttactaattg tctataaaaa ttacaattag
    12721 taacacctac atatggaatt agccatagta gacctattcc aagagtagct attgaaagta
    12781 tataccatcc tataaaacta aagtttagta caaataattt aagtacattt cctttcatca
    12841 tatctaaact agcacccatt gatttaaata cacccatatt atccttgtct tcacaaatta
    12901 agaaagtagc ttgagaaaca tataatagca atatataaac cggtaacata agaacaagta
    12961 ttataagaac tagagttatt ccagtagaaa taacagtccc tattccaaca ttatcacttg
    13021 ataaatattc aattgctcca ctagtaagta aaactccaaa tgataaaaac gctcctataa
    13081 ttgtgattat aaaagaataa atataaacaa taattgattc aaataaataa aaaccaaaac
    13141 ttcttaaata aattctccca cttacaaaga tatctcctat cttgactttt tcatctttag
    13201 taattttcaa gaacatacat gtaaatcctg ctgaaacgca aaaagctatt acagccacta
    13261 caatagtagt caatggttca aaaggtaatc ctacaattga tattaatact atcaatgcaa
    13321 tatatactgc catagaaatt gcacatatcc cccagtttcc agataactga gctttagata
    13381 ctcgttttaa ttcttgcctt gataccataa aaaacacccc tttcatttta taaaaaaact
    13441 tttaaaaata taaataatct tttaacgtat aatattaata tatcatattt tcgactatat
    13501 aagatacaat tattcaccat gttttatcaa gatataccat aattgtatta ctagttttat
    13561 atagtcaaca ttaccaaata catcaaataa atttgattaa ttattcaaca agtaataaaa
    13621 taatttctat taattatact ttatttagta aacaaattat ttaataaaaa aattacctaa
    13681 tacagtaaat taattggtaa gataaattat tt
//
LOCUS       DBGJFU010000101        13660 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.101, whole genome shotgun sequence.
ACCESSION   DBGJFU010000101 DBGJFU010000000
VERSION     DBGJFU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.101"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            53..1183
                     /locus_tag="ACOIAS_002775"
     CDS             53..1183
                     /locus_tag="ACOIAS_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGZ2702476.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(1300..2268)
                     /locus_tag="ACOIAS_002776"
     CDS             complement(1300..2268)
                     /locus_tag="ACOIAS_002776"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HGZ2702477.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            2603..2791
                     /locus_tag="ACOIAS_002777"
     CDS             2603..2791
                     /locus_tag="ACOIAS_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702478.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            3051..4250
                     /locus_tag="ACOIAS_002778"
     CDS             3051..4250
                     /locus_tag="ACOIAS_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HGZ2702479.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIESDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            4302..4805
                     /locus_tag="ACOIAS_002779"
     CDS             4302..4805
                     /locus_tag="ACOIAS_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGZ2702480.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            4912..5229
                     /locus_tag="ACOIAS_002780"
     CDS             4912..5229
                     /locus_tag="ACOIAS_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702481.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            5387..6346
                     /locus_tag="ACOIAS_002781"
     CDS             5387..6346
                     /locus_tag="ACOIAS_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGZ2702482.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            6461..7246
                     /locus_tag="ACOIAS_002782"
     CDS             6461..7246
                     /locus_tag="ACOIAS_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702483.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDKKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            7538..8857
                     /locus_tag="ACOIAS_002783"
     CDS             7538..8857
                     /locus_tag="ACOIAS_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGZ2702484.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(8932..9360)
                     /locus_tag="ACOIAS_002784"
     CDS             complement(8932..9360)
                     /locus_tag="ACOIAS_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702485.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(9451..10827)
                     /gene="hydG"
                     /locus_tag="ACOIAS_002785"
     CDS             complement(9451..10827)
                     /gene="hydG"
                     /locus_tag="ACOIAS_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HGZ2702486.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(10828..11913)
                     /gene="hydE"
                     /locus_tag="ACOIAS_002786"
     CDS             complement(10828..11913)
                     /gene="hydE"
                     /locus_tag="ACOIAS_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HGZ2702487.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(11949..13157)
                     /gene="hydF"
                     /locus_tag="ACOIAS_002787"
     CDS             complement(11949..13157)
                     /gene="hydF"
                     /locus_tag="ACOIAS_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HGZ2702488.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLIVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(13172..13423)
                     /locus_tag="ACOIAS_002788"
     CDS             complement(13172..13423)
                     /locus_tag="ACOIAS_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HGZ2702489.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         5116 a         1641 c         2098 g         4805 t
ORIGIN      
        1 atcttattta taccaataga ttaagaattt aattttgaaa gagagaaaac aaatgaattt
       61 aaaagacttt aatggaaaga gattaaaatc agctagaatt tttagagcaa agacgataga
      121 acagttgtct aaagaaacta aaataaataa aaaagattta aaagcttttg aggaaaataa
      181 gtatgtacca aatatagaaa atacattaaa attatctaat atattaaatt ttccaaaaga
      241 gtatttttat aaaaatgaaa atataaatat cgttgtagag gatagccact ttaaccctca
      301 atctaaacta cctagagtag aagagatttc ttatagagaa aaaataatta taatacataa
      361 aatatattca tttatggaga attatattaa gtttccaaaa gaaaacttgc caaatagaaa
      421 agtaatgtca gatattaata taaatgatat agaatctctt gcatataaga ctagagagtt
      481 ttgggattta aatagtactc caatagtaaa tatggtaagt ttgttagagt ctaaagggat
      541 tataatatca ggtatgaatg tagatagaaa aggggcaact atctttactc aaaaacagag
      601 aattagtaaa gaatctaaat atctaatatc tctgggtaat gacaaaaagt cagcatcaat
      661 tagaaattat actttggcat gtgagcttgg atatataata tcaagtgaat taagaatacc
      721 aagtaagcaa ttttcagaag atgagtatgc ctgtgcattt ttattgccaa aagagagttt
      781 tttaaaagat ttgactcatc cagaagattt agattattat gtagaactaa agaaaaaatg
      841 gattgtacct atatctgcta tgatttttag agcatataat ctagaaaaga taaattacaa
      901 aaaatacaat tatcttatga atgaaatgga taaaaaaggc tggttgatag aagaaccact
      961 agacaaaatg aaaggttcaa gcccaacgta tttaaaaaga gcagttgaat tattgataga
     1021 aaataaaata atgagtgtaa atagtatagt atcaagttta gaggaatttg gaataaatct
     1081 ttatccagaa gacttagaat tattgatggg attgaaaaaa ggattgttat ctcaagaggt
     1141 aaataaaaaa agtaaagtta taaaatttga tggtaaaaaa taggtttata ttttagtatt
     1201 aaaattaaaa gcaggtacaa atgatttaaa cattaactta gttaaaagtc ttttcaataa
     1261 gtcatttacc tgcttttagt gtaagaaaga atattgaatc taaagtgttt ttgaaagtgc
     1321 aatatattcc cttaaaacag aacaagattt attaaattcg ttttgctcat attcagtcat
     1381 attgatttca ataatttctt caataccatc ttttcctata acacatggaa cacctgcaaa
     1441 tacatttctt tcgttatatt gaccttctag taatgtagaa actggaagaa cttttttctc
     1501 attgtgtagt atagctttta caatttctac taaagcagta cctataccaa attctgtaca
     1561 atctttacca tttattatac ttattcctgt ttttttattt ttttcaacta ttgatggtaa
     1621 gtctaaattg ctataagtac ttgggttgtc ttttatcatg tctaagattg gtttaccacc
     1681 aattgaaaca tgagaccaag gaaccatttg agaatctcca tgttcaccca tagaataagc
     1741 ttgaattgat tgttgagcga ttccagtttc ttcagataaa atccttctaa gtcttgttga
     1801 atctaaagct gttccagttc caatgacttt gtttttagga aagccagatt tttcccaaat
     1861 ataatttgta actacatcaa caggattaga tatgactaca aatataccat caaagccaga
     1921 atcaactatg ggttttacta tagattttat cattccaata gttggtctta gtaaatctaa
     1981 tctagttgtg ttttgtctac gtaaaggccc ttctgaagag tctgcaacac ttatcactac
     2041 tatgtcagaa tctttacagt cagaaaaagt tcctttttct atatgtactt tatgtggcag
     2101 atatgaaact gcatcagcta aatctaaagc ttgagctttt gatttagatt catctatatc
     2161 aatcataaag agttcatcac aaactccttg agttattaaa ctaaatccac aatgagaacc
     2221 aacatgccct gaacctacaa tagatatttt tcttggcttt attgccattt taaatcactc
     2281 cccctaaaat aacgatacat tgaaaattat agatgttatt aaaagctgtt tttggatatt
     2341 ttaatattct attatttcag aaaatttatt taatatgatt ttatactaaa atttttaaaa
     2401 aagcaatgtt aatatactct ctattagtga atattaaagt attatatgta tattaataat
     2461 tattaaaata tgtaatgtca ctgatagtta gaatatttaa gtgctaaaag atgtgtataa
     2521 ataataatga tgtcagttta taatgatatt aaattgagat atactgtttc tctaaattaa
     2581 aaaataaagg agtaaatgtg ttatgaatta tcatacaaaa gaagaattgg tggaagcact
     2641 gagagtagta tcttcaagta ttattaattg tggaaaagga cagaagaaat tttctgagga
     2701 gacatctcat catacacgtt ttaagaatat tatagaagta atgtatattt caaaatcatt
     2761 aataatggat gaaattagta aaagagattg aagcttattt tgcaatttaa catgttttaa
     2821 tatacatttt attatataag aaaataagta aacatacatg ttataaaata ttggaataat
     2881 atacaattat aaatttgtta acactaatat ctatatgtta aaatatagta gaagatgtta
     2941 gtaaataata ggatgagttc tattttaata caattaaatt aagcttagtt tgaaatttgt
     3001 atctaaaaag taaagtaatc tagaattaag aaaatggaga tagaattata atggaaaagt
     3061 tatattatac aaatcagtat ataagagatt ttacagcaga aataattgaa ataaaagaag
     3121 tagaaaataa gtatcatgtt ttattagata agacagcttt ttttcctggt ggaggaggtc
     3181 agtttggaga cctgggaaaa atagagagtg atgatgtaat aaatgtgtat gaagatggaa
     3241 aaaaaatata ccatgtatta gataaaaaac ctataaagat acataaggta aagtgtagta
     3301 ttgattggga tagaagagaa gatggaatgc atcaacattt tggacaacat gtattgtctg
     3361 gatgtttttt taaaactttt aatgcaaata cgacaggttt tcatttggga aatgagtata
     3421 gtacagtaga tatagaaggt aatttagaag aggataaaat tagagagata gagttgtttg
     3481 ccaatgaaat tattcgtcaa aatatacaag tagagacatt acttccaacg aaaaaagaac
     3541 taaaaaagat ttggttaaga cgtgatttgc caaatactga tgaagagata agagtaataa
     3601 aaataggtga tttagatacg aatgcatgtt gtggagtgca tccaaaatca actggtgaat
     3661 tgagaatgat aaaaataaag aaatgggaaa aacataagtc aagtacaaga atagagtttc
     3721 tagcaggtaa aagggcagtg gatgaagtat tgaagaggga tatatatttg actaaaatat
     3781 gtagatattt aagctgtagt gaggaagatg ctataaatgg tattgtaaat ctaaatagta
     3841 aagtggaaga ggctttaatt aaaaaaagaa ggcttgaaga agtagtatct acatatcagg
     3901 tcaaagaaat gattgaaaaa gcaaataagg ttggcaatat ttcggtcatt aaaaaaattt
     3961 atgatgatga agatttgaaa tatgtaaata agatagcaaa taaaattgca gaatatgaaa
     4021 atagtattgc attattagct gttaaattta atgataaaat aaatttagtt tttgcatgtt
     4081 ctaaaaattt aaaagatatt aatatgggag ttttattaaa ggatgcaata agtcttatag
     4141 atggcaaggg tggtggaagt aaagtattag ctcaaggagg aggtaagaat aatggaaatt
     4201 tagaggctac acttgattat gcatttatga agatagaaaa aactctataa atatattata
     4261 taaaaaacct tagtagataa aatagatatg gaggaataag tatggagcaa caaaaggact
     4321 acatagagtt atatgaaaat gaagaactaa tagagataag agaagcacgt atggatgatt
     4381 tggatacaat agcaaaattt aattataatt tggctaaaga aactgaagga aaagaacttg
     4441 atatggatgt tttaactaaa ggtgtaaaag cattgttatt agatgagaga aaaggaaaat
     4501 atcatgtata tacagtattt gataaagttg ttgcacagat tatgtataca tatgagtgga
     4561 gtgattggag aaatggaaat ttcctttgga ttcagagtgt atatgtagat aaggaatata
     4621 gaagaaaagg tatctttaat tatttattta attatataaa aaatatatgt gataaagatg
     4681 aaaatatagt tggaatgaga ttatatgtag aaaaagaaaa cataaatgca aaagcaacat
     4741 atgagtcttt aaatatgtat gagtgtgatt ataatatgta tgaatacgaa gtaatacatt
     4801 cgtaatataa atttgtaata ctgttatact gatgttataa agtattgatt atattgacga
     4861 ttatatgaaa taaactggat attataatta gaatttttag ggggtataaa aatgggcgtt
     4921 ttaaaaggaa tgttttatgt atttttattt ttagacttag tgtcaatatt ctgtttcttt
     4981 ttcaataaag gaaaaatagc atctaataaa attgtgttta atgcaatagg agtacttaca
     5041 tttgtacttt gttttatgct ttttagttat tatcctaata ataatcttat tggaaaattt
     5101 attgcttctc tattttttat atttggagtt gcaggagtta tgttgaaaga aaaaaatttt
     5161 ttatatgcta gacttctttt aactgtagtt atagtttttt ctacattaag attgttcgtt
     5221 attcaataaa atgtatttgt atgaaaattt attaaagtct acacatttaa gtgtaggctt
     5281 ttttgatttt ttgtttgaaa attaaggaaa ttttaaggaa attttaaaat cgtcttaaat
     5341 atttattgat atacttaaaa tattaatagc gaaaggagac ttatcagtga tagaaagtaa
     5401 aaagtcagaa aaattgttac tgtcaattat atacataata tttttggtat atattttaat
     5461 gattataaga ataattttat ttagagatgt accaatttac gctattttca agggaacgtt
     5521 taggaatgta aatttaattc cattttatac aatatatcaa tttatagtag atagtaatct
     5581 tgactttatg aaagctactg taaatattat agaaaatata ggtatattta ttcctatggg
     5641 aattttttta cctatagtat gtaaaaatct aaataaaaaa actataatta tagtaattat
     5701 acttgttagc ctagcatttg aattaacaca atatattttt gctcttggaa gtagtgatat
     5761 agatgatgtt attttaaatt cattaggtgg cataattgga attaatattt acataaacat
     5821 gaataaatta tttcccaatg atattaacag atttaaggct ataataggta ctagtttagt
     5881 tttaggagtt ataggattag gagttatttc taaaaattat cataacttac taacttttaa
     5941 atttagacct gatagaaaaa tatcaaaaat attaattgaa gaaaataggg aaataattaa
     6001 agatataaat aaagatacag tagatatagt tgggacattt gagagcttta aaaaaggaat
     6061 aataactatt aaagcaggtt ctaataacaa agtaaagaga ccagagaatt taattgattc
     6121 tgatggaaat ataagaattt atttaaatga aaatacaaaa ttagtatctt atataattac
     6181 caaagaatca aaaatggata tagttaaata tgaggaattt gatgtgaaga atttagattt
     6241 attaaggaga tatgatacta ttaatgtttg gattgataaa gaaaatcagc caaaggataa
     6301 acatggtatt atggctagta aattattaat tggattgtat gaataaaata ttaaacaaga
     6361 ataaaagaat aacaaatata aaaaacgaaa aaactatttc tataattatt tttacgttgt
     6421 gcataaaata agaacattag tgaaggagta gagataaatt atggagataa taaaatatat
     6481 cttgtacttt tttatatatt catttttagg atggacagta gaaagtatag gatgttctat
     6541 agcaagcaaa agaataatca acagaggttt tttaaatggt ccaatatgcc ctgtttatgg
     6601 ttttggagcg gtaattgtga tatcactatt gggaagattt aataatgtag taatagtatt
     6661 tctattagga atgattttaa ctacgatact agaatatttt acaggattta ttttagaaac
     6721 cttatttcat gctaaatggt gggattattc tgacaaaaaa ttcaatataa agggtagagt
     6781 atgtttaaaa aatgcaatat actttggtgt aatgtctgtt ttaataataa gatttataca
     6841 tccatttata aaatattttg taagtataat tccatataga atattaattt caatggctat
     6901 aattacaaca ctttggacta tacttgattt gattgttact attataacac ttaaaaaatt
     6961 agatataaag ttaaacttat tagacgatat aattacagac cttaatgata taaatgtgaa
     7021 attggataaa tttgatagag gagaaataca agctttattt aaaacaataa ataacgacga
     7081 attagaagtt agagaaaaaa taaacaaaat aaatagtaag ttggaccgta tagagtctaa
     7141 tattatttta caaaaaagag ttataaaagc ttttccacat ataaaacata aaaaacaaca
     7201 agagcaacta gaacatttta aaaaaattat taatgagaaa aggtaattat acaatttaat
     7261 gaagacgaat agaataatgg tatttataag atgattttca gaggactcta taaaaataga
     7321 atagagtcct ttattttttg aaaaataatg tttatttgat aaaaataaaa tatattgttt
     7381 ctgaataaca aatattttga aaattataaa aacatatatt gacaatttgt gtcaaaaata
     7441 ctaacattaa gtatataaag aattttatat gagaatatct ttataaatcg caataatttt
     7501 aaaacataaa tttatttaaa gaggggatga tttgccaatg agcgagataa ctagtcaaat
     7561 gataagtaca gaagaaaaaa aatatgttgc taaaactcaa aagatacctt attatccagt
     7621 agcatttaaa tcaggtgatg gtgcaatgct atatgattat gaaggtaatg aatatgtaga
     7681 ttttttagca agtgcaggct ctgcaaatgt aggtcatggt aataaggaaa tctcacaagc
     7741 agttaaagaa caaatggatg atataacaca atatacactt gcatatttcc atagtgatcc
     7801 tcctgtaaaa ttagctgaga agcttgtgga aatagcccct ggagataatg ataaaaaagt
     7861 actatacagt gcaactggtt ctgcttgtat agatgctgct ataaaattag caagagggta
     7921 tactggaaga acaaagataa tatctatgtg tgaatcttat catggaagta cttatggagc
     7981 tatatctata tctgctttaa gtacaaatat gagaagaaaa atgggtccat tgttaccaga
     8041 agtttatcac ttccattatc ctgataaaaa tagaacagct aaagaatgtt tagatgagat
     8101 agaatatgct tttgcacact atctaccagc agaagaggta gctgcaatat ttatagagcc
     8161 aattgcagga gatgcaggaa taatagtgcc tccagtagag tgggtacaag gtttaagtaa
     8221 aatatgtaaa gaaaatggaa tattacttgt aagtgatgaa atacaacaag gtatgggaag
     8281 aacaggtaag tggtttggta tagaaaattt tggtgttgaa gcagacctta tagttcttgg
     8341 aaaatctgtt ggaggtggat tacctctagg agcagttgtt ggaagaacag aaataatgca
     8401 aagtcttgat gcaccagcac atttatttac tttagctgga aacacaacag tatgtgtggc
     8461 tgctttaaaa tctatagaga taattgaaaa agaaaactta cttcaaaaga gtatagaaat
     8521 gggagattat attaaagcag gatttgaaaa actaaaagaa aaatacgata taattggaga
     8581 aataagaggc ataggattat ctataggagt tgatatagtt aaaggaaaag gttctaatga
     8641 aaaacatcca gatgctacag ctaagatatg ttatagatgt atacaaactg gattaataat
     8701 gatattctta ggacaatcta ctttaagagt gcaaccacct ctagtaataa ctaaggagca
     8761 agtagataaa gcaatgaata tcatagattc tgcaatagat gattatttaa atggtagaat
     8821 tggtgatgaa gtatatgaag taactcaagg ttggtaaaat aaatattaaa tgaaaatagg
     8881 agttatctca attttagaaa attatttctg aaaagagata actcttttaa gttacccaca
     8941 aataagaaaa ttgtttgatt cattaaagac tatatttcgg atgtcgtttt tgtgagtatc
     9001 agagacagta ttacgagaat aattaccttt attcgtacta tctgttaatt tagttataat
     9061 taagatagct aaaagaataa gaattactgt gtataaagca ataacatatg gttttctttt
     9121 atatttgtat ttgttattga aaaaagaatg gccataatta tttttattta ctttagatgt
     9181 atttgtattc tttttcttgt tacctttctt tttatcatcc ttttttctat ttttagggta
     9241 aggaatgact ttgtgtgtat tatcaagttt gatattttta tttttttgaa tattgttttc
     9301 caggctagct tttttttctc caaattgaat agttttattg gtattgttgt tatctttcaa
     9361 tgttaatctc cctcccttaa actttccctt tagataatat tactactaac ctatggaata
     9421 tacaataaat aatatataag taataaaatt ttataggtat aaatctcttt ctccacgctc
     9481 taaggcagtc atattgttag atactatgtt tcttatatct tctctttcta tcttacctat
     9541 ttcttctgcg ataagttttt tacccataat tttaagttct tcatctccat aatcgattaa
     9601 aaattcattt aatgtagtaa gagcatttgg tgtacataca ttatggattt gtcgactttt
     9661 agctaagctc atgaatctat cgccagttct acctttacga taacaagctg tacaataact
     9721 aggtatatat ccatcagtaa tcagttcttt aagcacctca acagggcttc tatggtctcc
     9781 aacttcgaat tgttctgtat tgtcaccatc ttcatatgct ttatatcctc caacacctgt
     9841 tagagaacct gcactaattt gtgatacacc atattttagt aactcatttc tcatttcagc
     9901 agtttctcta gtggacatta tgattccagt aaaaggaaca gctaatctag ttatagctac
     9961 tatttttttg aacatatcgt cagaaactaa gtgaggaaaa tcttctaagc tcataccttc
    10021 agcttttttc aatctaggga aagatatagt atggaaacct acaccaaatt tttcctctaa
    10081 gtgttcgttg tgcatcataa gtcctagaac ttcaaattta gggtcagata aaccaaataa
    10141 aactcctgca ccaacatcat caacaccagc ttccatagct ctatcaaaag ctgttaaatg
    10201 gtaataatag tcatttttta tagattgacc atgcattttt ataaatgttg gtttgtgata
    10261 agtttcttgg aataatatat aagttcctat tcctttttct tttagtaatt tatactcatc
    10321 tacacttgta gctgctatgt taacgtttac ccttcttata tttccgtttt cattatatgt
    10381 tgaataaatt gcatctaagc aatctaagat gtaatttata tcacaatttt tagggtcttc
    10441 accagcttct agagcaagtc ttttatgacc cattttttct aatattttaa cctcttccct
    10501 tatctcttcc atggtaagtt tttttctttt aaatttgttg catttactaa aaccgcaata
    10561 aacacattca tttacacagt agtttgatac ataaagaggg gcaaataaaa ccactctatt
    10621 accatatatt tcatttttta tttgcccagc tatactaaat aatctatcta gttgtttttt
    10681 atcttctact tcaagaagtg tagctatatc tttataacta agtttttccc ttctatcagc
    10741 cttatcaagt accttttcaa tatcatcaga agttgaattt tttgcatctt ctaataaaga
    10801 attaataagt tcatggttta tgaacattta caaacgcctc cattcaatat tatcaccgcg
    10861 agaatagtca acttcaagtc ctaatgactt gatgttttct tctaaacttt ggtgatattc
    10921 acaagcttct tgaccagtaa aggctttgtt atcgtaaaga gaatattgtt ttctaaattc
    10981 ttgaggagaa aggtttggca taattacatt gcaaccagct aaaagacctg catttctacc
    11041 actagggttt atagatgcca aggctgttgt agcgggtaat aaaacttttg gaagtagaag
    11101 tcttgtaatt gaaagcataa gtaatgtctt ctctaaatct ccatgtttat aatccttaaa
    11161 aattgtatca tggtgtggaa tgaaaggtcc tattccaacc atatgaggat ttaactcttt
    11221 taggtataat aaatcacgaa caaggtcttc gttgttctga aatggtgatt caaccataaa
    11281 tccagcacct acctgataac caatttcttt aaggtttttt aggcattctt ttctagttct
    11341 aagctcaatg tttggcggat gtaatttttt ataatgactg tctgtagcag tttcatgtct
    11401 taaaagatat ctatctgcac cacataaaaa gtatttttta tacgaatcat aactcttttc
    11461 tcctagtgat agagttatgg cacaatcctc atatttattt tttatacttg aaacaatttc
    11521 acatatttta tcatcagtaa aatatgcatc ttcaccacct tgcaaaacga atgttctgta
    11581 tcctatttta taacctatat cacaacattc aagtatttca tcaagtgaaa gcctgtatct
    11641 tatgatattt ttattagaag attgtattcc acaatagtag caattatttt tacaatagtt
    11701 tgtaagctct ataagtcctc tcaagtagac tctatttcca taatgtttat ttctcatgtc
    11761 gctagcttgt ttataaagat agtttcttaa attttgttcg aagttatatt cctcattgtc
    11821 gatattttct attaaatata gtagttcatc aaaacttaat gaattaatgc tgtaaagttt
    11881 atctataaag tatttaatct tatttttagt catattcaaa tatataatta ttaagtatag
    11941 ttaaattttt aataattgaa aagttcaagt gatctttcca aaataccttt tacatatgct
    12001 attaagatac catagtttac aataggtaca ttgaaagatt ttgccttttc tattctagat
    12061 aacattccag ctctattcat catacaagct ccacagtgaa ctacaagggc atatttattt
    12121 atatcttcag tgaaggatac tccagaacta aattcaaaag taatgttttt gccagttttc
    12181 tttctaatca tattaggaat ttttactgtt ccaatatcat cagtttgtct atgatgggta
    12241 catccttcgg ccatcaagat tttatcacca tcttttaaat tttctaaagc ataagcgcca
    12301 ttaatcaatt ctttaaggtc tcccttttgt ctagcaaaca atatagaaaa agatgtaaga
    12361 ggaatatctt ttggtgtatc tttatctact tgtggaaata cttgagagtc agtaataaca
    12421 agttttggtt ttttgcctaa attagataga gtttctttta aactatcttc ttttgtaact
    12481 atagaaattg caccactatc caaaatatct cttataactt gctgttgagg aagtataagt
    12541 ctaccttttg gagctgcttt atctattgga acaactaaaa caattaaatc atttggttca
    12601 attaaatctg ataccaattt gaactcagtg ctatccttag gcaaaacttt tattatttca
    12661 ttttttaaat tttcaattcc aattttatct gttgatgaga cagaaacaac tggacattta
    12721 actttatttt tagttaagtt taaaatttct gattgattct taatagtatc aattttgttg
    12781 agaattagaa tatgagggat attcttatca ttaaactttt caattagtga caaatcttct
    12841 tgtgatatac caatctggca atccactact agtaaagcta tgtctgtctt ttctaaaaca
    12901 tctagtgatt tacctattct aagttcacca agttcaccaa catcatcaag accagcagta
    12961 tctataagaa cacaaggtcc tataggaagg atttccattg gtctaaatac tgggtctgta
    13021 gtagttccag caatatctga gactattgca gcactttgat ttgttatagc atttattatg
    13081 ctagatttac ctgcatttct tttaccaaag agacctatat gtactcttac cgattgtgga
    13141 gtagaattta gactcatttg tactcaccac cttaaacttc tttttttgat atactagttt
    13201 ttacagatac atgaggtatg ttgcccaatt taccagtcaa actattaata gtatccattt
    13261 cgccaacaac tgctatacat acgattgaga caccttcttc ttcgcaagga atgcccattc
    13321 ttcctttaat aataccttta aaacctgata caacctcatt gaatttaaat tgacattctt
    13381 ttggctcttc caaaatagca cttataactg caaccttttt cataaatatc ctcctaactc
    13441 agaaaatatg tcttaaacaa atccaaaatc aaaaaacttt tctttgtaaa gaaaagtttc
    13501 tacaaaagaa aagttattaa ttacacaagt ataatgtgct atattaaact atttccttac
    13561 agattagata actcttttct ggtagggtta gcttgtaaaa atttaattta acaacaagta
    13621 actgaagaaa atttttatac aatttatata aattataata
//
LOCUS       DBGJFU010000102        13428 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.102, whole genome shotgun sequence.
ACCESSION   DBGJFU010000102 DBGJFU010000000
VERSION     DBGJFU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.102"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            239..940
                     /locus_tag="ACOIAS_002789"
     CDS             239..940
                     /locus_tag="ACOIAS_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HGZ2702490.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1210..2028
                     /gene="larE"
                     /locus_tag="ACOIAS_002790"
     CDS             1210..2028
                     /gene="larE"
                     /locus_tag="ACOIAS_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HGZ2702491.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2083..3231
                     /locus_tag="ACOIAS_002791"
     CDS             2083..3231
                     /locus_tag="ACOIAS_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HGZ2702492.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3367..4755
                     /locus_tag="ACOIAS_002792"
     CDS             3367..4755
                     /locus_tag="ACOIAS_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2702493.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4784..5482
                     /gene="walR"
                     /locus_tag="ACOIAS_002793"
     CDS             4784..5482
                     /gene="walR"
                     /locus_tag="ACOIAS_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism DNA-binding response
                     regulator WalR"
                     /protein_id="HGZ2702494.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5485..6918
                     /gene="walK"
                     /locus_tag="ACOIAS_002794"
     CDS             5485..6918
                     /gene="walK"
                     /locus_tag="ACOIAS_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HGZ2702495.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6940..7677
                     /gene="truA"
                     /locus_tag="ACOIAS_002795"
     CDS             6940..7677
                     /gene="truA"
                     /locus_tag="ACOIAS_002795"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGZ2702496.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    7788..7995
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8248..9447
                     /locus_tag="ACOIAS_002796"
     CDS             8248..9447
                     /locus_tag="ACOIAS_002796"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGZ2702497.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9694..10740
                     /locus_tag="ACOIAS_002797"
     CDS             9694..10740
                     /locus_tag="ACOIAS_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HGZ2702498.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10848..12011
                     /locus_tag="ACOIAS_002798"
     CDS             10848..12011
                     /locus_tag="ACOIAS_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2702499.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12100..12480)
                     /locus_tag="ACOIAS_002799"
     CDS             complement(12100..12480)
                     /locus_tag="ACOIAS_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HGZ2702500.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(12483..13142)
                     /locus_tag="ACOIAS_002800"
     CDS             complement(12483..13142)
                     /locus_tag="ACOIAS_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGZ2702501.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13248..>13428)
                     /locus_tag="ACOIAS_002801"
     CDS             complement(13248..>13428)
                     /locus_tag="ACOIAS_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGZ2702502.1"
                     /translation="NFKNLSSSFKLIKLNEFEEYPDFILKLGDKKSFVESVIKINKSN
                     RKSIKNLILKLSKKP"
BASE COUNT         5392 a         1330 c         2301 g         4405 t
ORIGIN      
        1 tacttattat taaccatgaa catttgtttt gtgctatcat atttatataa gggaattgtc
       61 ataaaaaata tcttgttcat acagttttat atagaaaaaa gaaatgaatc ttttctttat
      121 ataagtatat tttgtaaatt gcctctatat ttatagttta aataatagtg gtattaaaaa
      181 tctgacaaca aaaataaaac tttcaaaaaa ttaaatctaa aaagaatagg agataaatat
      241 gagtattgaa gatttatatg attttgaatg tgttcctttg aacaatgaga ttgatattga
      301 tgttttattt gaaaagatga tagaagatgg aaaagaaaaa gaatatacac caattatagt
      361 cattgatgaa aaagtagggc tattaagaaa aaatatagat atgattacta aagagtatgg
      421 ttctcttgaa aactatagag agtattgttt aacaaaatat aatgaaattg atgtaaatca
      481 attttttgat tttaggaaga gtgagatttc agaattatta aaggagctaa ttgatttaga
      541 agatgatttt tatgattata aacattttag acccattatg aatatagata tatttcatga
      601 atataacaaa gtttacatag ctaaaattcc aacaattaaa ccatatgaag tatttgcata
      661 catacctatt ggcggattta atgattgtcc tagagatgaa gaacatatag ctattgcaaa
      721 atattggtat gaaaagcatg gaatctttcc aatcgcaatt ggatgtgata cagttcaata
      781 ttctgtaaaa aatttaacta aagatggtaa aaaatttgat gacttatgta ttgaattagt
      841 attttattgt gaggatatta ttatacaagg gtatgaaacc ttaaaggctc ttaaggatgt
      901 attaaaacgc tctacaatag tgcttttttg gtgggattga tattctatca tttagatagt
      961 attaaattgt tagtcaccaa gaatccactt gctttagtta tagggagtgt gtcaaactag
     1021 gtttttatac acagtgtagc atagtaatat gtttaaatta caaaggttaa aaaaatactt
     1081 taatattttt aatattataa ttaatagatt taaggtaatt ataatttata aaataaatac
     1141 tttatatgtt ataatcatta agaatgtaaa aaaataatat atagttttat agttcatatt
     1201 tttccagata tggaggtaaa ttttgtgaag gaaaaagaaa aattagataa attaaaaaaa
     1261 atgttacttg aattaggcag tgttgtagtt gcttattctg gtggagtaga tagtaatttt
     1321 ttgcttaagg tcgcaaaaga tacattaggt gaaaatgtta tagctgtgac tattcatgca
     1381 atgatgcatt caagtaggga aattgaagag gctaaacaat atactcaaaa ttttggtgtt
     1441 aaacatatca tattaaatat agaaaacttt gatttaaaag aatttaaaga aaatggtgtt
     1501 gatagatgtt atcattgtaa aaaatatata ttctctaaaa taaaagaggt agcaaaagaa
     1561 cataatataa agtacatagt agatggaaca aatatagatg atttaggaga ctatagacct
     1621 gggcttaagg cattgagtga attaggagta attagtccat taaaagatag tagtttaaaa
     1681 aaagaagaaa ttaggtcgct ttcaaaaaca ttaggattaa atacttttaa taaaccatca
     1741 tttgcatgtc ttgcgagtag gataccttat ggagttgaaa taacagatga aaatttaaga
     1801 atagtagaaa agagtgaaga atatctatcc aatttaggtt tttctcaatt tagagtgaga
     1861 atgcatggag atatagctag aatagaagtt ggacaggaag agctaggtaa attttttgaa
     1921 aataacaatt ttaataaagt tgatactaaa ttaaaaatat ttggctttaa atatgttaca
     1981 ctagatatgt caggatataa aatgggaagc atgaatttaa atgtgtagat tattttaaaa
     2041 ataaataagt tttatagtta atataaggaa acaggaggtt ttatgaaaaa ttatacgtta
     2101 atatcgccat gtttttttgg aatggaaaaa atgctggcta gagagattac taatttagga
     2161 tatgaaataa taaaaacaga agatggaaga ataacatata aaactgatga gtttggaata
     2221 gcaaagtcaa acatgtggtt gagatgtgct gaaagagtac atcttaaaat tgctgaattt
     2281 gaggcaaaaa gctttgatga attatttgaa aatacaaaaa gaataaactg gtctagatat
     2341 ataccttatg gagctcaatt tcctatatca aaagcttcct ctataaaatc aaaattatac
     2401 agtacaccag atgtacaagc catagtaaaa aaggctatag tagaaagctt aaagaaaagt
     2461 tatttggagg atggtttgct taaagaagat aaggagaaat accctatttt tgtatttata
     2521 cacaaagata aagtaaccat ttctatagat acaacaggag atgctttgca taagagagga
     2581 tatagagaaa aggcaaataa agctccaata agagagactt tagctgctgg gcttatttat
     2641 ttaactccct ggaaagcagg tagagtttta gtagacccta tgtgtggttc tggtactata
     2701 ttgattgaag ctgctatgat tggtataaat atggcacctg gcttaaatag agagtttata
     2761 tcagaaaagt ggagaactct tgataagaaa atatggtggg atgtaagaaa agatgctttt
     2821 aataagatag ataatgaatc taaatttaag atttatggat atgatataga tgaagaatct
     2881 atagatatag ctagagaaaa tgctgaaata gcaggtgtag atgaatatat agagtttaat
     2941 gtaggagatg caactcaatt taaaagtgaa gatgagtttg gatttataat cactaatcct
     3001 ccttacggag aaagattaga agataaagac agtgttaaac agttgtataa ggagcttggt
     3061 tatgcattta gaaaattaaa aaattggtcg tattatttga taacttctta tgaagacttt
     3121 gaatatgagt ttgggcaaaa agcagataaa aaaagaaaat tgtataatgg tatgttaaaa
     3181 actaactttt tccaatatcc gggtcctaag cctcctagaa ataataaata agttttacat
     3241 gatttatttg taaaatataa aattttatta aaataagtaa aacattatga tgccctgaaa
     3301 cgtctaatta ttgtgaggag atttttgtga acaaatttaa agatttatag gattaggagg
     3361 agttaaatga aagtgttaaa gcttattact gtagtagccc tgatgatgtt atttgttact
     3421 ggatgtaata taaaaactga atctccagaa caattagcga agatacctga ctatgatgat
     3481 acaaaaaaag tattgtatga tggtattgac cagttactaa aaccagattc atctatgata
     3541 ttgccttcta atacaaaaga agtaggaaaa ataaataagg tagatttaaa cagcgatgga
     3601 attgatgaat tagtagtatt tgaacaaaaa gaagatttaa gtaataatgt gagtcaggta
     3661 gggtttgtaa ccttgagtta caatggagaa aaatacgtac ttggagacca ttttttagaa
     3721 aatggcgaat caatagaata tgctaatttt tatgatttgg actcagatgg atataaggaa
     3781 gttatcttat tggttaaatc caaagacaaa acgaatatgc atatatataa agtagtagat
     3841 aatgaaataa caaaaatata tgacttggat gcatcatggc ttcaaaataa agaagatttt
     3901 aatgacatga aagtaaaaat tggatatata gatggagatg acaagttaga catattgatg
     3961 cttcatttga ataataagac taatgaaatg tttgcaagtg ttgcaaattt tgatgggaaa
     4021 atgaaaatca aagattctgt taagtttgaa aatgtaaaaa acttaagtga attatatata
     4081 acattaggta atgttgcttc atctgttgga aattcttctg cagctataaa aggtattgta
     4141 cttgatattc ctatattgaa agataataac tatattacgc agatacttta tatgaaagat
     4201 gggaaaatta acaaagcttt tagtgattat gataaaacta ttactaaatc ttattatata
     4261 cctgtagatg atattgacaa agataagatt atagaaatac ctatagttgc aggcagtaca
     4321 ggtaataata aaaatactta tagttctaaa tcatctgcaa ctataagttg gtatagatgg
     4381 aatggaaaag aaggtgctga ctcaagttta atatttacaa gtcagatata ttataattat
     4441 aaatataatt ttaaattatt tattccaaat aatttatttg ataaaatact tgttgaacag
     4501 gaatttgttg gagaaaaagc agtatttaaa ttttactatt ttgactattc agaccgtaag
     4561 aatttattta atatagtagt agagagtaaa aataaacttg aagatagaaa aaatacaggt
     4621 actcaaaatt ctattgtact tcaagagagc gatgaatata ctttcttact agttgtaaac
     4681 aatgcaaatg aaatggataa gctagatatg actatagacg cattaaaaga atatttctca
     4741 ttaatatatg gttaatatta tactcaattg gaggttttat tttatgaagg aaaagatact
     4801 tatactagaa gatgaaattg gtattagaag ttttgttagt ataaacttaa aaagggaagg
     4861 ctatgaaata gttgaggcag gtactggaag agaagctata gaaaaaatga caacagaaaa
     4921 ggatattaca atagctcttt tagatgtaat gcttccagat ataagtggta tagaagtatg
     4981 taaatttata agggagaatt ttgaccaagt aggtataata atgcttactg ctaaagctca
     5041 agaagatgat aaaatagaag gatttatctc aggagcagat gattacatca taaaaccatt
     5101 tagtataaaa gagcttttag tcagagtttc tgctcttctt agaagagtag caaaggatga
     5161 ttcaagtgtt aagtcgagtg agatagtttc tccaccattt atacttgata tagacaaaag
     5221 aaaattattt aaaaatggaa aggaaattga gcttacacca actgaatttt caatagtgaa
     5281 atatctaata tcaaatgcta agcagtcgtt aagtagagac cagatattag acgaagtatg
     5341 gggaactaac tatttatatg attttaaaat agtagatgta aatataagaa ggataagaaa
     5401 taaaatagaa gatgacccat caaaaccaaa atatatacaa actatttggg gttatggata
     5461 ctgttttaga aaggaagaat aatagtgtta cattttgaag gtctgagaaa aaaagtaata
     5521 aaaaactatt ttataataat tattataatg gttactctgt ttgaaggatt gttcatgttt
     5581 tacatacaaa actactatta tgattctgtc aaacagctac ttgaatctga aataaaatat
     5641 gctgatgaat ataatgctat tacaatggaa actacaagtt ttgagaaaaa agtaaaaaat
     5701 atatttgata aacaaccctt aactaaaaat tcagaatttg gaatttcaat aatagataaa
     5761 gataaaaata ttatattaga ccaatatggt tttaaaagta aagaaaaagc aaactatgaa
     5821 gatgttaaca atgcattaaa agatataaaa actaaaaatt taacacctta tacttataga
     5881 ataccagata caggggagca tgttatgagt atctccttac cacttaaggt aaataatata
     5941 atagaaggtg ttgttagata tacagtatca ttagatgcta ttgataatgc aatattaaag
     6001 caagctacat ggcttatatt agcaggaata tttatactta ttatagctat actaataagt
     6061 cttaaatttg ctgagactct tataaaacca ttacgagagc taaagaaatt tgcaaatgaa
     6121 ttggctgtag gaaattataa tataaaatta gaaaagatga agattgtaga tgatgaaatt
     6181 ggtgatttag cacagacatt tgaacacatg gcacatgaga ttgacaaaag tgaaaaatta
     6241 aaagaggaat ttatatcatc tgtatctcat gaactaagga ctcctcttac atctataaag
     6301 gggtggagtg aaaccttagg ttatgaaagt ataacaagag aagaactaga cttaggttta
     6361 ggcattatac aagatgaaac agaaagactt ataaaactag ttgaagaatt attagacttt
     6421 tcaaggcttt cttcagacag gataaaactt catgttgata tagttgatgt tgaaggttta
     6481 attgtaggtg ttgtaaatca acttaaagtt aaagcagctg aaaaagatat atcactttta
     6541 tttgaatttg aaaatgaatt tatagagaac attcaaggag ataaaaatag attaagacag
     6601 gttctcataa atttaataca aaattcattt aaatttacga gtcaaggtgg ttacataaaa
     6661 gttgtagctt ctcaagatga agagataaca accatatcag tagaggataa tggttcaggt
     6721 atagaaaagc aaaatttaaa taaagtgtta gataaatttt tccaagaaga ttataataag
     6781 gctggtagtg gattaggact agcaattagt aatgaaattg taaaacttca tggtggaaga
     6841 atgaaaatag aaagtgaaaa gaatgttgga acaaaaataa cttttaatat aaaaaataaa
     6901 tttgctaaac aagcataaat tcttagaaag aggattaaga tgagaaacat aaaaatgata
     6961 gtagcatacg atggttcaag atataaagga tatcaaaaat taggtgacaa taatatgact
     7021 attcaagaaa aacttgaaaa tgttttaagt aaaatgacta atgaaacagt ggagataata
     7081 ggttctggaa gaactgatat gggagctcat gcaagaggac aagtggtaaa ttttagaact
     7141 aattgtatgg attctttgga taaaattcaa aaatatttat atgaatattt accagaagat
     7201 atagttgtaa aaactgttga agaagttgat gaaagatttc attcaagata caatgtaaaa
     7261 tcaaagactt atatgtataa aattgacaat aataagtatc ataatccttt tataagaaaa
     7321 tatgctactc atgtaagtaa aaaattagat ttagatagaa tgagaaaagc aagtgagtat
     7381 ttagtaggag aacatgattt tactagtttt gcttcttcta aatctaagaa aaaatcaaat
     7441 gtaagagaaa tttattctat aaatataaaa gaagatgata atgttataga aatatatgta
     7501 gaaggaaatg gatttttgta taacatggta aggataatag taggtgcttt gatagatgtt
     7561 ggtctaaaaa gaagagctcc acaagatatt aagtatatgc ttgaatcaaa agatagatgt
     7621 caatcatcag atactgcacc agctaaagga ctatgtcttt ggaaagtaac atattaatca
     7681 attatataaa actctcttta aaataaaaat aaaataaatt atatagttga cataaatgga
     7741 aaaataaact attatatatt ttataagaga gatataaaac tttatattta aatcctgtga
     7801 gaaagagagt aacttctatg tgttttatag agagctgagg atggtggaag cttggcaaat
     7861 aagtattagt gaaaagaact ttggaggata ctaattgaat ttagtatgtt agtaggtaat
     7921 ctcgccttaa gagaaagagt ggataagttt atcttatcaa ttagagtggt aacgcggata
     7981 tatttcgtct cttggttttt ccaagaggcg ttttttaata tatttaatta ttagtgttta
     8041 tctggtatgt tgaacattta ctaattgatt agctaagttt atcaataaga gtggtaccgc
     8101 ggatatttcg cctcttagta ttttaaaata ctaaggggtt tttttagtat taaaatgatt
     8161 ttatgtgagt ctaaattctc agagtcattc aatattaaga atttctataa aataaaaatt
     8221 ataatttaag attgggagga attaaaaatg aaggaaaaag tagtattagc atattcagga
     8281 ggacttgaca catcaatcat aataccttgg ttaaaggaaa attatgaaga tatagatgtt
     8341 atagctgttt gtggaaatgt aggtcaggaa gataaaatgg aagatgtata tgaaaaagct
     8401 ttacaaagtg gagcatcaaa ggcatatgtg gatgatataa gtgaagaatt tgtaactgaa
     8461 actatattta aagctgtaaa agcagaggct aaatatgaag gaaaatactt attaggaact
     8521 tctttagcaa gacctataat agctaaaaaa ttagttgaag ttgctcataa agaaggtgca
     8581 aaatatatat gccatggatg tacaggaaaa ggtaatgacc aagttagatt tgaagctaca
     8641 attgcagctc ttgacccaac tataaaagtt attgcacctt ggagaatttg ggatataaaa
     8701 tcaagagaag atgctataga ctatgctgaa aaacataata taaaggtaac agcaactaaa
     8761 gctaaaatat attcagtaga tgccaacctt tggcatgttt caacagaagg tggagatata
     8821 gagcacctag aaaatgaaca taaaaaagat gtttataaac aatgtgtaga cccagaagat
     8881 gcatgtgatg tagctgaata tgtagaagtt tactttgaaa aaggtgtacc taaaaaggta
     8941 aatggcgaag aattatctcc agttgcactg attcataagt taaatgagtt gggttgtaaa
     9001 catggaattg gtgttataga tatagttgag aatagacttg ttggtatgaa atcaagaggt
     9061 atatatgaga ctccaggagg aacaatcctt tatgaagcac ataatatttt agaaagtgct
     9121 actttagaca aagatacatt acatttcaaa caaatggtat cttataaata cggagaactt
     9181 atttacaatg gactatggta ttgtaaatta agagaatcta tagatgcatt tatggaacaa
     9241 actcaagata atgttacagg aacagtaaaa gttaaacttt acaaaggaaa tataaaacct
     9301 gctggaatat ttactgaaaa tgctctttat gatgaaggaa tatcttcttt tggaaatagt
     9361 gaactttatg accacaaaga tgcagaagga tttattaatt tattcacttt accattaaaa
     9421 atcagagcta tgaaagctgg aaaataataa aataaattat ttaagagtgt ataaaaacga
     9481 tttttaggtt tttatatgct ctttttttat ttgaatttat taaattatac agtaaatagt
     9541 ataaaaaaat atattatagt tccaaatggc ttaataatgt gaaaatataa gacctaaaat
     9601 ataaatttta taaatacaat agttattgac tctctcattg taagagggtt tatactgaat
     9661 ctaatgaaaa tttatagtga ggagatagat tatatgagaa aaataattga aattaaacat
     9721 cattcacatg attatgagtg tatgtggaat ggtatagaag atttatatat aaataaaacc
     9781 ggtgaatacc ttccaaacga ttttttcttt ttactgtcag gttttggttc attttgttat
     9841 atgaaaacaa ataaagctga tttgaaaaga atggtagctt tgggagatgg aaggacaaaa
     9901 cagatgtata aatttttagc accaattgtt ggatttgaat acaaacatca tgagtttaaa
     9961 aagtatgagc aagcattaaa gaaagcaaaa tctgaaattg atgatggata tccagttatt
    10021 ttaggagctt tagatatgta ttatctacca tattataaga agctatatca taaagaacat
    10081 attccttttc attatatatt aatggtagga tatgatgatg aaggaatata tctgtatgat
    10141 tgtggtagaa cagaattatt acatctttct tatgataaat tacgtgaaag catgaattgc
    10201 aattcccctg gattaagcaa agaaaatact atttgcacaa taagaatgaa tacttcaaag
    10261 aataaatata acattgctag agaagctatt gctataaagc gtgatatgtt tttaaatcca
    10321 cctaaaggat ttttgggata taaaggtttt gaaaagttta ttaaagagtt gccacagtgg
    10381 aacactgaat tggataagga tgattatgat aagattttga ccaatatggt tatgtttttt
    10441 ggaacagtac caacaattcc aaatgcaatc aaaggaatta aagaaccaga tactgttcaa
    10501 tttaaaggtg gatttgataa aatgagtaga atgctcaaca gtattgggga ggaatacaaa
    10561 aacaactctt ttatacaagt agctgatatt tttgataaaa ctgctattat aattgagaaa
    10621 atatccaata ttataataga ttacttaact caaacatgtg atgatacaga acaacttccg
    10681 ttattgttct ctgaggtctt agagcatatg aaaacaggat atttaatctt ggatgtataa
    10741 aaaatatatt gttgacccta acattacgta atagtgtaat atgaaaatat agcttaaata
    10801 tattagtagg tattgatggc tgtaaaatta aaatttgagg tgtggatatg agaacagtga
    10861 aacaagtttc tgatttaact ggaataagtg tgagggcatt acattattat gatgaaatag
    10921 gtcttttaaa accaaataaa attacagatg caggatatag actttatgat gatgaatcta
    10981 ttaaaactct tcaacaaatt ttatttttta aggagattga tattccttta agagaggtta
    11041 aggaaataat gtcaagccaa tattttgata aggttgaagc tctaaaaaat caaaaaaaat
    11101 tacttatttt aaaacgaaag agactggatg aattgataga gcttataaat caaactctaa
    11161 gaggagaagg aaatattgat tttaaagagt ttgatatgag cgagtatttt aatgttttag
    11221 aagaatttaa aagagagcat aggaataagg taattaaaat ttatggtagt gtagaaaaat
    11281 acaatgaata tatagaaaga gttaagtcta atgaagagaa aattgctaaa atggctataa
    11341 aacaatatgg aactattgag aagtttgcta aagctataaa aacaaatttt agcagtgata
    11401 tattgaattt aggagaaaaa tttgatagat ataaaaatga ttgtttaaaa gaaaaacatc
    11461 caaaattaaa agaattatat agaaagcttg tagaagattt aagtaggaat ccttcttcaa
    11521 cagaccttca agaaatagca aaagcgataa cagatatatc taaaaaagat tatgaaattt
    11581 tctctatgga tactggtgat gacaactggt attatatggt tcaaaattat ctagtaaatc
    11641 ctatgtggat agaagaagta gataaaaagt atggaagtgg agcaggaaag tttattggac
    11701 aagtactaaa gacttatcta agagatagaa aaccaaagat aaatacattg tatgaaaaac
    11761 ttgtagaaga tttgagtaga gattgttctt caagggaagt tcaaagcatt gttgaagaaa
    11821 tagacaatga aatgaaaaga agtaatgaat tttataaaat agacaatggt gaaagatact
    11881 ttgattatat gtcagagctt tatttacaag attctaacta tataaaagta actgataaga
    11941 attatggtga tggtgcatct aagtttattg gagaagcttt taagatttat tttgataata
    12001 ataattgttg aaaatttgga agtaaaaaat atgtaagttt tataaaagca caaaaataag
    12061 gatgtagatt agctccatct atatccttat ttaaagttat taaaatatat agtttacatc
    12121 aaacttaaca ctttcaataa tatttcccaa atatgttttt atactttcaa cttcttctaa
    12181 agattttaat ttattttcag tataaattgc tttgtccaga atattaaacc ttaatcctat
    12241 ttcatcaaag tactcatgat ttaaaacttt gtaccaatgg tttttttctt tattccaact
    12301 cgtagatatt gaatcagctt ctttttgagc tttttcccaa tcttcatttt ctatagattt
    12361 ttctaaaaca gatatattgt ctttatatcc ttctgtaaaa tcatatagtt tattgttaac
    12421 gtaaaatcca aataatacaa ataagatagt ccatagtata acaaatgttg tagatttcaa
    12481 tattaatcac cctttttatt ttgaataaat attttgtcat tttcatctaa aacacaaata
    12541 agaacatctt taaaagtttc tatgtgatta gattttaaaa tacccattaa ccagttagta
    12601 tctttttgag ctatgtctag atttttattt attattctac catccagtat aagtgggata
    12661 ggtaaatgat taaaagctct tggaggagta ctattgtagc ttgatgctgg tacaacactt
    12721 agattaccat cagtctccaa aatagcatat tgtacatctt ttaggttaaa gtatccttga
    12781 atcctaagtt gttctaataa ttcatctata ctaattcttt cttttttaag ctctttatat
    12841 acaatttttc ctttatctat taatacagaa ggtttacctg ataaaaatga acttaatttt
    12901 ctgcttttta atgataagaa tgaaattaga gtctgcatga ttacaagtcc agatattgct
    12961 gcaacaccat ttatgattgg tatattatta ttttccattg ggacagatgc aacttctgca
    13021 attaatagtg ttataactaa atcaaaacag ttcatttcag ctatttcacc ttttcccatg
    13081 actctaagtg caattagcac tgttatgtat aatataatac ttcttattaa tactactgtc
    13141 atatctatca attactcctt taaaaaatat ttacacttag ttagtatatc ctatataaaa
    13201 aatttaattc aaagtataaa aaaccttaaa taattggatt caactattta aggttttttt
    13261 gataatttta atattaaatt ttttatggat tttctgttac ttttatttat tttaataaca
    13321 gattcaacaa aactcttttt atctcctaat tttaagataa aatcaggata ttcttcaaat
    13381 tcattgagtt ttattaattt gaaagaagaa gatagatttt taaagtta
//
LOCUS       DBGJFU010000103        13405 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.103, whole genome shotgun sequence.
ACCESSION   DBGJFU010000103 DBGJFU010000000
VERSION     DBGJFU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.103"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..591)
                     /locus_tag="ACOIAS_002802"
     CDS             complement(<1..591)
                     /locus_tag="ACOIAS_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2702503.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(924..2138)
                     /gene="dpaL"
                     /locus_tag="ACOIAS_002803"
     CDS             complement(924..2138)
                     /gene="dpaL"
                     /locus_tag="ACOIAS_002803"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGZ2702504.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2981..3289)
                     /locus_tag="ACOIAS_002804"
     CDS             complement(2981..3289)
                     /locus_tag="ACOIAS_002804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702505.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSNVSVSERII
                     "
     gene            complement(3366..5171)
                     /locus_tag="ACOIAS_002805"
     CDS             complement(3366..5171)
                     /locus_tag="ACOIAS_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2702506.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5567..5944)
                     /locus_tag="ACOIAS_002806"
     CDS             complement(5567..5944)
                     /locus_tag="ACOIAS_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGZ2702507.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6849..7334
                     /locus_tag="ACOIAS_002807"
     CDS             6849..7334
                     /locus_tag="ACOIAS_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702508.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7534..8073)
                     /locus_tag="ACOIAS_002808"
     CDS             complement(7534..8073)
                     /locus_tag="ACOIAS_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2702509.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8066..8479)
                     /locus_tag="ACOIAS_002809"
     CDS             complement(8066..8479)
                     /locus_tag="ACOIAS_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2702510.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8515..9636)
                     /locus_tag="ACOIAS_002810"
     CDS             complement(8515..9636)
                     /locus_tag="ACOIAS_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGZ2702511.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10235..11887
                     /locus_tag="ACOIAS_002811"
     CDS             10235..11887
                     /locus_tag="ACOIAS_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HGZ2702512.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            complement(12270..13196)
                     /locus_tag="ACOIAS_002812"
     CDS             complement(12270..13196)
                     /locus_tag="ACOIAS_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HGZ2702513.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4475 a         2230 c         1487 g         5213 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaagtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttagtt tgttgacgtt ttattttgat atttcacttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt tatttaattt tatttagttt ttattttgaa
      781 tttttcattt gctttacttt ttcatttatt tagatttttc atttgtttta gtttttattt
      841 tatttaaagt tttcacttat ttaagttttt attttcctta aatttttcat ttatttgtta
      901 agtttttatt ttccttaagt cttttattta ctttggcatt caccattcca aactatatct
      961 ctatatttat cagggtcagt atctccttct gtacttatta ataatacttt agagttctca
     1021 tctagtttca aagcttctct taagtctttt aaatcttcac tttcaaggat tgaaactaat
     1081 gctccagttg taactgctcc agattctcca gagattactt tctcatctcc tcttagaggg
     1141 tttcctaata ttctcatacc tttagcagat acccagtctg gtgctgaaac gaatgctgat
     1201 gaataatttt ttaacacttc aaaacctata gtgtttggtt ctccacaagc aagccctgcc
     1261 attatagttg gcatatctcc tccaacaaat cttggttttc catctccagc ttctgctgat
     1321 ttatagtaac aatcagcctc atcagcctct actactactg ttattgggca ttcatcatat
     1381 attgaagcaa catacccttg aactgcacca gcaagtgaac caactcctgc ttgaacaaat
     1441 acgtgtgttg gtctatctac tccatattct tttaattgtt ctatagcttc tttagccata
     1501 gttccatatc cttgcattat ccatgctggt atttcttcat atccgtccca tgctgtatct
     1561 tgaaccatta ctccatggtc atcagcttct gcataatcat tagctaatct tactgcatca
     1621 tcatagttta agtctgttat actagcatct gcaccttctc ctttgatttt atttaatcta
     1681 aattcagaag aaccttttgg cattaataca acagattttt gacctaattt attagctgtc
     1741 caagcaactc ctctaccgtg atttccatct gtagctgtaa agaaagttat ttctcctaat
     1801 ttttctttta tttctttcga agtcaatttg tcatacccta actcagatat atctgtattt
     1861 aatctttgag ctaagtatct acccatagag taagaaccac ctaaaacttt aaatgcgttt
     1921 aagccaaatc tataagattc gtctttaaca tatattccgc ttacgcctag tgattttgct
     1981 aagttatcta atttaactaa aggagtcttt tcatattgtg gaaagctttc atgaaaagct
     2041 ttagcctttg caatctcttc atcttttaaa aattcaattc ctttttcttt ttcctctgat
     2101 ttaggtaagt tatttaattt ccattttatt tctctcattt attttatatt cccctttcaa
     2161 atttttcggt gtcattttaa ttttttatct ttgtgcctta tagcaaagca attaccgtgc
     2221 caatattatt tattctaaaa acaatttttt tatgttggaa ttacatactt ttataaaatt
     2281 tctttaaagt ttataaattt tgagtgtgaa aaataagatt tataaaagaa aaaattctca
     2341 tattgggaaa tttttatcaa tacgagaatt atcaattcga gaaatcgttt atttatctta
     2401 attcttcctt tattatgctc ttatttttta tatgtctatt gttttgtcag atatattact
     2461 acttttattt tctataaata tatggctata accttcttac gcataaactc tataaattta
     2521 tatactttat tacccaaata ttctttatta cccaaagcct ccaaaatggc ttaaatcttt
     2581 tcatagcttc ttacagaagt ttacatggtc gtccccccaa taagcaaaag tgcaatatgt
     2641 gtaccattgt gccctacttg cgactattga gctgtcgaaa ttcgcaactt cgcatttatc
     2701 tgatgaaatg cactatacaa aatattcata acatccacaa cagagttaaa gttacctatt
     2761 catattcact gtaaaaattt cttccacagg aaatgtaatt ttaaccaatc ctattgagac
     2821 aaactcacca cttatttttt tgaactcacg cagtaataca tcacttgccc gaaaaaaagc
     2881 ctcactttca tcatctccag taagtgctat tgtgcaatgg ggtacccaac gattggggtg
     2941 gtaccattcc caaccatttg aatcaaaatc ttgtagtacc tcatataatt ctctctgaaa
     3001 ctgatacatt gctattcatt ataggtgata caaacaccgt tttagtatca ttaaacatgc
     3061 ctactgaacc aatgtaagca ggtattacct tatggttttg tgcaaacccc tttagcttgt
     3121 ctatgcaaca agcctcatcc acatcattga aacaagccaa agtaagatga ggtcttgttt
     3181 tccattctaa gtacttcgta ctaatttttt catcagcgat ttttttagat aaatcaaaaa
     3241 gttgcttttc cgttttttta tcataatata gctctatcgc atattgcata ataacacctc
     3301 caccataaag taaaaatacc tatctataaa aacacacaaa tacctatcat tttaatatta
     3361 tattactact gttcctcttc caattttcta tatagtgtag ccaaacctat gcctaagtct
     3421 tttgctatct tcttcttcgt ctttgtatct cttccatact tattcaacaa cttgtttatg
     3481 taagtcaact ctaattcttt aattgaaagt atcctttctt gattctctac tatccctcca
     3541 actatatcat cttcaaatat tatatttaca tccttatttt tataaatatt tccattaata
     3601 ttataatagt ttaaaatatc taatggaagc atatctttat atattattcc atcatctcca
     3661 accaaattca tcatatattc aactgcattt tccagttccc ttatatttcc cggccaatta
     3721 tagttaatca acatattcat tacattttct tctattttat ttaactgaat acctaattct
     3781 aaagaatatt tagaagcaaa cttcatcatt attgcctcaa tatcatcctt tctttcccta
     3841 agaggtggca aatttatagg aattacattc aatctatagt ataaatcatc cctaaactta
     3901 ccttcattta ctaatttcaa taagttttta tttgtagcag caatcactct tatatctaaa
     3961 tctataagtt tatttgaccc aattctcgca aattttctct cttgtaaaac tctaagtaac
     4021 tttgcttgta aaggcattga caaatctcct atttcatcaa gaaatataac accttcattt
     4081 gcaagttcaa atttacctac tttccctcca ctactagctc cactaaatgc accttttata
     4141 tagccaaata gctcactttc aaggagattt tctggaatag cagcacagtt tatcgcaata
     4201 aatggcttgt tccatctgct accttcagca tgtatagccc tagcaattag ctctttaccc
     4261 gtaccacttt ccccagttat aagtacagtt gaatttgact ttgctaattt cttagttctc
     4321 tcttttactt ttaacattgc ttctgaattt ccaattatag actctatatc actatttccc
     4381 cagccactat tcacttttcc atgtccttta tgattgatat atgtcttgtc aaatattatt
     4441 attttgtcgt attgatttat atatggatat accggaatta ttttggcatt tatattataa
     4501 attttgttat caatatcaat ttctagtaat tcatgtccat ttgaggaaga ttcaacagat
     4561 actatattta ctatattttc tattatattt ttatctatat ctaatttttt tagagctata
     4621 ttgtttatat aagatatttt gctatttatg tctagtatta taacgccttt ttcaacattt
     4681 tcaatgattt ctatcatcat atccattttt ctactgctat tttttgacgc ttcatgttct
     4741 tctattttca tagaaattaa atcgcatata tgattgacaa attttaaata attatctatt
     4801 tttgcactta tttctacttt tttagtttta tctgtgctaa tagcaccaat tactcctata
     4861 gttcgattat tatactttat tggaactgct attattactt tatttaggca tttcatgtaa
     4921 tgggaacatt ctatacaaag ctgattatca catatatcca atacgactaa ttcctgacct
     4981 gtctgtataa cattgtcata aataaagccc tcagtaacaa cgctttcatt tatctttgat
     5041 ttatacagac ctgtaccact aattcttatt aatctatcgt caactatttc aatatctaca
     5101 tctaacacac tagctactgt ttcagtgtat tttatcattt cttgttgaaa ttcttttaat
     5161 ttacttaaca tagtttcccc cattagaatt taatttacca aattatcagt ttagctatat
     5221 tttaacatat agtcactctt taaaatatat ataaaacatt acttatttat atatttgcgt
     5281 taaaacagac aatattaatt tataaaaagt ttgttttata tagtaacctt aattataata
     5341 tgcagacaat aaaataaatc acaatacaat aataaaaaaa agctttcctc atataacaaa
     5401 tatatttttg acataaaaat atacattata tgagaaaagc tcaatagtta aatatttaga
     5461 tagattttta tatagacttt tttaatataa acttttttaa ttgtagactt tttttattag
     5521 tttttaattt ttattagttt tgttattggt tttattgtta taatacttat aatactgcca
     5581 ttgcttcaat ttctaaaact gcatcttttg gtaattgagc aacttgtaca catgaccttg
     5641 ctggtggatt ttctttaaaa tatgtagcat atacttcatt aatatcatta aaatccttta
     5701 aatcttttac gaatactgta gtcttaaata ttttatccat acttgtacca gcttgttcta
     5761 atattgcttt tacattatct aaagaagctt ttgttgcttc ttttgggtca gatactaact
     5821 cgccagtctt catatctatt ggaagttgac ctgatgtaga aactatgttt ccaacttgaa
     5881 ttgcttgaga atatggtcca attgctcctg gagctttctc tgtagctatt actacttttg
     5941 acatgacttc acctcctatt attaagttat atttacagga aaataaaatt tcctaaacac
     6001 caatattaat acgaaccttt agattaaact tcaaatctaa aacttcaaat aatcaatact
     6061 tagatgatta tgtttatttg tgtcttgtta acttttatta aagcaattaa cgtgccaaac
     6121 tttatttata tataaaattt tgtgaagtca ttcaaaatag ttacttctga attttttcgt
     6181 aaaaaaacgc tctaaaatta cttttttatg atgtcaattt ttttctcata tctagaaatt
     6241 taccaacatg agaattcttg ttttgagaat ttacagatta aagttatttt ttaaccttaa
     6301 attctgaacc tcccatgact aaagtcacag ggttcctgct tcatagaatt ttgcataatc
     6361 aagttattac atatgaaaga actatagttg acaatcccac tattataaat aataaaacct
     6421 ttacccatac cttatttacc ctagttatca tagagcctat tgaaaatata acagctaaat
     6481 ataataataa ccaaaaaatt atcaatatct accactgttg ctgaaaatcc aaaaaactac
     6541 cttttgatag tattacttaa caatctaatt aatctcataa taaatttaca acttataaat
     6601 aaattgttat attggccgta tctcaacaac tttctccttt cctagtttta aatcttttat
     6661 agaattgtac cacgtctaag taaattttca tactattaag tcttatttct aataaatcat
     6721 tagctctcat tctatgccca ataatgtaag attgcagtaa ttttattgat tttaatagtt
     6781 ttatttaata tacttatatt aattattttc atatgaaatt attatttata tagggggatt
     6841 tttttaatat gagcaaaagt gaattaacag cagaaacaac agaagaaatg ttagaagtac
     6901 taagtggtaa agattatgat attgcatgtc atttacatga acttggtaaa tcattagatt
     6961 gtaaaattga accaaaaaca ggtgctcgtt cttacaaaat agtatattca actaagaaac
     7021 caaaacgtag cttatttact attgaatgta atgaaaaaaa atggagagtt aaagcaaatc
     7081 tttttcatct aaatacatat aaagatgctg tggaggaatg ctctaaaact attaaagata
     7141 gtataactaa aactcgtact tgtaagaaat gtaattcaaa gtgtattgga ggttcttcgt
     7201 ttgaattaga tggaaagtct tacctgactt gcataggaag tggtcattat tttgcaaata
     7261 tggaagaaat ggattggaaa aacctagaaa aattaattac taaagaaaat aatattatgc
     7321 aggaatctgt atagtttttt aagggtaatt attaaaatat atgagtaaca acagatactt
     7381 aaataggcat aaaaaaataa agggtataat aaggatatag aataaaaata aatgatatag
     7441 cacaaagagg ctgtctcaaa atacagataa gcctcttttt attgttgaaa tgataagaat
     7501 atattcaatt ttatacaatt ttatacaaac atactacgcc aacttaccca tttcatatgc
     7561 ctgtttcatt gccttacttc ccttaatatc acctacattc caagcaccta caccatatac
     7621 tatacctttt tccttagaac catttagaca tgatgtaaat cctctaaatc cttctattgt
     7681 tctttccata tcagccacat tttcatctgc tgctgtaacg ataaaataga aatctttatc
     7741 acaaatttca gtatatctag agcaagttct atcaatcata gttttcattt gcgcattcat
     7801 agtataaaaa taaactggag tcgctaaaac aatcacatct gccccaatca ttttttcaag
     7861 aatttctccc atgtcatctg tctgtgaaca atttgcttct ttttcataac aagcaccaca
     7921 tccagtacaa tagttaatat ctttatcttt taaaaatatc ttctccactt gatttttaga
     7981 ttctaatacc ccttgtataa actggtcaca caataaatca gaatttccac cttttctagg
     8041 actagctgat actattaata catttttact cataagatac tgcctccatt ttcttatttc
     8101 tactgctctt taatactttt tctttctcaa taataccttc ttcatatcta tcaatcttaa
     8161 aatttaatcg ttccaaagtt ttttgcattt cttctattct ttttgctagt tggtttctct
     8221 gctctattaa aagttctttt ctagctttga tagtactgct cccttgttga aacatagcta
     8281 catactcaat taatgtctca atagaaagac ctgcacctct catacacttt atgaaattaa
     8341 cccactcaca atcttcttct gtaaaagacc taattccact tttatttcta tttacaggtg
     8401 gtattaaacc aattcgttca taatatcgta aagtatccgc tgaaagctcg tattttttac
     8461 taacttctga aatcgtcata catatcttct cctcttgata ttgtatagat tgaattattt
     8521 ctcaaaggtt tctgctacat tctttaaagc atctataatt ttaatgtgtt gaggacagct
     8581 ttcttcacat tgtttacatt caatacaatc tgaggccttt ccataagttt tagtataatt
     8641 attatagtag accatctgtg ttgaaaatcc agtattaaga gcttgttttt ctgcattgta
     8701 caacgcaaaa tagtttggaa tagcgatatt ttttggacag ccttctacac aatactgaca
     8761 ggcagtacaa ggaattaaaa ttgattcgtt gataatctct actgcttcat ctataatatt
     8821 atattcctct tgtacgaatg gtttgaaatt ttgcatatat cctgtattgt ctaataattg
     8881 ttccatattt gacatcccac ttaagaccat cataacatta tctttacttg ctgcaaaacg
     8941 tattgcccaa gatgaaattg acatatctgg attgtagtca ttcaataatt tctctgcttt
     9001 ttctggtatc tttgcaagtg tacctccttt tactggctcc ataacaatta caggcttatt
     9061 atgcttctct gccacctcgt agcattttct agattgtatg ctttcattat cccaatctaa
     9121 ataattaatc tgtaattgca cgaagtctac ttctggatgc tcactcaaga ctttatccaa
     9181 taattctgca ctatcatgaa aagaaaaacc aatattcttt atttttcctt cttttttctt
     9241 ttcttcaata aacttaaagc tatcaaattt tttagctact tcataatgtg acacgcctat
     9301 attgtgtaaa agatagtagt caaaatagtc tacaccacat ttttctaatt gctcattaaa
     9361 aatggtttct tgctcttctt ttttctttaa agccattaat ggaagttttg tagctatagt
     9421 aaaactctct cttttgtgtc tttttactag agattctcta agtgctatct cactttttcc
     9481 catgtgatac acataggccg tatcaaaata tgtaaatccc ctttctaaaa aagtgtctac
     9541 cattttattt aaatgttcca tatcaatagt tgtaacatca ttttcatctt ttattggtaa
     9601 acgcattaat ccaaatccta atttttttgc ctccattgtt ttacttcccc ctttaaaact
     9661 tgtttgtttt ttctctctac tttaactatt cttacaagta tataattctt gtataattat
     9721 actttactac ttggagttaa cttcaagtca atagattttt ttatttttaa gacaaatatt
     9781 ataaaaatac agttctttaa tattaatcca ttacatgttt ctttcaccct ccaattctca
     9841 aacttcacaa actccaacat gtaaagaaat atatacttta agaaattcag tccattaata
     9901 ttactacaaa tactttttag atatgtttaa aaagaaattt cacctaaaat atttataaaa
     9961 gatttagaaa taggtcttgt gtctatttta aaatatataa aacagtgata aagacttatt
    10021 tataagataa aaaatgaata gtgtataccc tttttaaaaa gttttttcct ttttatattt
    10081 gtgatacaat gttaaatata aaaaggaaaa ttttgtatta aagaaatctt atttgttgat
    10141 tttgtaactt caaactcagt atcaatttga ttttaagatt atattttatt aagtatgcta
    10201 aataacaaat ttttaaagat tgtgaggtaa aaatatgaat aagaaaaatt tagctatatt
    10261 aatatcaggt atgatgtttt tttctagttc ttcagcaatt tttgcagaca atactacaaa
    10321 gcaagaaaga ttaataggta aaacaagata tgaaacagca gtagaagtaa gtaaattggg
    10381 atgggttcaa tcaaaaacag ccattatagt taatggtaat tctattcaaa gtgcgctttg
    10441 tgcaaatcca tttgctaagc taaagaatgc acctatactt ttagtaaaca acaacagtat
    10501 tgaaaattca actaaggctg agcttaaaag gcttggtgta gataatgtgt acattgtaga
    10561 tagtggaaat agcatttctt ctaaagtaga aaatgaaata aaatcactta acataaaaat
    10621 aaataaaata gtaggaaaca atatatatga aatgtctaca aatgtactta aagaaataga
    10681 taagataaaa aagatagaaa atgttgcagt tgtaaatgga ctaaagggac tatctgatgc
    10741 agttagtata gcttctccag cagcaataca taatatgcct ataatattag tatcagatga
    10801 aaacagcaat gatagtagta taaatttctt aaacaatagg tctataaaaa aatcttacat
    10861 agttggagga aataagattt tatcagacaa tacagtaaag aaatatccaa acagagaaag
    10921 aatcagtggt tcagatagaa acaatactaa tgcaaaaatt attgataaat tttataaaga
    10981 taaaaaaata aataatgtat ttgtagctaa agatggtatt aaccaagaaa atgcattagt
    11041 agattcatta tcaataggag tactagctgc taaagaaaat tctcctataa tgatatctaa
    11101 tggtagcctt gtaaatagtc aaaaagaatt tattaaaaat aaagatatag atgaaataac
    11161 acaagtagga ggaggatata atcaaaaagc atttgaagat attttaaaac taaaaaatgt
    11221 gaacataaga aataaaacta aaaaaatatc tttaatacag gctaaagaaa tattaaaaca
    11281 agctcttcca ggaagcaata ttttagtttg ttattatgat gaagatgata atgaatatga
    11341 ctgcaaagtt ataaaggata atatcctata tgagatagaa attgatgcct actctggtaa
    11401 aataatagaa attgaaaaag atgatgactt aaatgataat aatttaggta cacaaaatat
    11461 atcattggat aaagctaagt ctatagcatt agatgctatt cctaatggta atttaatcta
    11521 ttgtaaatat gatgctgaag ataacgaata tgaggctaaa gtaataaaaa ataagaaaac
    11581 atatgaatta gagataagtg catttgatgg aagaattgta gatattgaca tggacaatga
    11641 ttctgatgat gactttgatg ataatgataa gaataactct aatactaatg acaacagttc
    11701 tattattagt gctgaaaaag ctaaatcaat aatgctagat aaagtgcctg gtgggaaaat
    11761 agtaaaattt aagtatgatg aagatgacaa agaatatgaa ggtgaaataa taaaaggaaa
    11821 aactgaatat gagataacaa ttaatgcaat tactggtaaa atattagaat ttgaaataga
    11881 tgattaataa aaaatataaa tagatgatta ataaaaaata tatattctat taattattac
    11941 aagcaataaa gccataaata ttgaaatttt caatatttat ggctttattt tattttttta
    12001 atttaaatcc acattatgcc attatatata tctatgcact cttgtaaaat gggttatatt
    12061 tctccagtat attaaaaaaa gtgagtgtct caaaataaat cttctaactc ataaagcact
    12121 ctcctttgta ttaaatcaaa tatattttca tcatttaaac aattaagaag agacttctct
    12181 ctattttgag acaactccat tttaatacac tatttataca taaacatatt ttaaacaact
    12241 aaaaagcaac taatatctta ataaatatct caatacatat cttcataaca catatcacca
    12301 taattacaag cagataaaga actctcctca tttaaatacg actcaaattc atacccaaac
    12361 agaattgttg gttctatata tatttccata tctgtatatc tccatcttct tacctttgcc
    12421 tctataacct tgtaaaaatc ttctaaacta tacccttctt ttaatctttt atctataagt
    12481 gccttagtta tactcaaatc agctttaaaa ttttttcctg attcactatt taattttctt
    12541 ataataattt catgtatgct ttctggattt ttgtaagtag aatcttcagt caaatcttca
    12601 tccgaatttt caaaggtatt tttatttaaa ttctttttat ttaaattatt ttttaataat
    12661 tcttttttct ttgtctcatt ttctgtttca tacccagtat gaattatcaa atcataatct
    12721 ttgtctatac tctcttgatt tatacggctg tatatgttgg tattactgtt cttaaacaac
    12781 tcatttctat gtttatattt cattgttttt tggttattgt taatagttgc atcaatgtat
    12841 ttctctatat ttttgaaaat atcaccattc tttgttacaa tttctgcatt actactttca
    12901 gaacaaacat aactatatac agaacaacaa tttcccttta ctcccctact tattaatctc
    12961 aatataccta aatctataaa ttcatttaca agtctactta ttgtagatat agatacatca
    13021 aggtcagatg cagctttcct tactgtcatc ataaattgtc ctcttggcag agttcctttt
    13081 aatctttgta tattttcttt tctcattaca tagtgattaa accttattct atttacatct
    13141 cttttttcat taaaatataa tactgctgaa tttaattgat aaaaactatt actcatattc
    13201 ccactcctcc aatttaaata tataatttgt tgtaaagttt ttagattttt aaataactta
    13261 ttaaatgatt atttttatta aaaagtattc tttgatattt tcattttaaa gttttaagta
    13321 taataagtta atagtgtaga tttaactttt ttatttaaac aataaactta atattatttt
    13381 taatataaaa attttattaa attta
//
LOCUS       DBGJFU010000104        13386 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.104, whole genome shotgun sequence.
ACCESSION   DBGJFU010000104 DBGJFU010000000
VERSION     DBGJFU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.104"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(132..2132)
                     /locus_tag="ACOIAS_002813"
     CDS             complement(132..2132)
                     /locus_tag="ACOIAS_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2702514.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSELSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            complement(2163..3881)
                     /locus_tag="ACOIAS_002814"
     CDS             complement(2163..3881)
                     /locus_tag="ACOIAS_002814"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2702515.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKHDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(4107..5345)
                     /gene="fabF"
                     /locus_tag="ACOIAS_002815"
     CDS             complement(4107..5345)
                     /gene="fabF"
                     /locus_tag="ACOIAS_002815"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGZ2702516.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(5374..5598)
                     /gene="acpP"
                     /locus_tag="ACOIAS_002816"
     CDS             complement(5374..5598)
                     /gene="acpP"
                     /locus_tag="ACOIAS_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGZ2702517.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(5652..6401)
                     /gene="fabG"
                     /locus_tag="ACOIAS_002817"
     CDS             complement(5652..6401)
                     /gene="fabG"
                     /locus_tag="ACOIAS_002817"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HGZ2702518.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(6401..7351)
                     /gene="fabD"
                     /locus_tag="ACOIAS_002818"
     CDS             complement(6401..7351)
                     /gene="fabD"
                     /locus_tag="ACOIAS_002818"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGZ2702519.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQVDLKYSACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQDTVPTGVGAMAAILGLDRSILENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLREAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(7367..8296)
                     /gene="fabK"
                     /locus_tag="ACOIAS_002819"
     CDS             complement(7367..8296)
                     /gene="fabK"
                     /locus_tag="ACOIAS_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HGZ2702520.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(8405..9391)
                     /locus_tag="ACOIAS_002820"
     CDS             complement(8405..9391)
                     /locus_tag="ACOIAS_002820"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGZ2702521.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(9417..10439)
                     /gene="plsX"
                     /locus_tag="ACOIAS_002821"
     CDS             complement(9417..10439)
                     /gene="plsX"
                     /locus_tag="ACOIAS_002821"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGZ2702522.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(10450..11007)
                     /gene="fapR"
                     /locus_tag="ACOIAS_002822"
     CDS             complement(10450..11007)
                     /gene="fapR"
                     /locus_tag="ACOIAS_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HGZ2702523.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(11214..11390)
                     /gene="rpmF"
                     /locus_tag="ACOIAS_002823"
     CDS             complement(11214..11390)
                     /gene="rpmF"
                     /locus_tag="ACOIAS_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGZ2702524.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(11415..11939)
                     /locus_tag="ACOIAS_002824"
     CDS             complement(11415..11939)
                     /locus_tag="ACOIAS_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HGZ2702525.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(12032..13228)
                     /locus_tag="ACOIAS_002825"
     CDS             complement(12032..13228)
                     /locus_tag="ACOIAS_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HGZ2702526.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3956 a         2624 c         1385 g         5421 t
ORIGIN      
        1 ctattaatat ttaattataa attacaatat tctttctgac ataatataaa ttaatctcta
       61 taaatataaa agtttattat tatttaaata tctaaattca aaatatgtga tttcaatatc
      121 aaatacttag cttaatttat attattataa agttttatcc cctctgaggt tatttgactt
      181 cctcctctac cagatttaga tgatatgtat ccacattcac ttaaataaga tagcaccttt
      241 ctaactttgc tttcagaaat ctcaatacct tcctgtataa gcttttcgat tataaaccct
      301 cttccaattc cagaggatga ctctcttctt tcatatagaa tcctcagtat ttcctcaaat
      361 tcttttcttg tataaccaaa tccatcttca taattgttag cacattctac ccttatatat
      421 ggtggtaagt catctctaaa aaccatacta tcacacatta gacttacata agaagcaaca
      481 ttttgtaact ctcttatatt tcctggccaa ttatattttt ctaaaatagt tataacttct
      541 ggcgaaagtc ttatattgtt tttcaaaaag aaattcataa gtgttattat atcttctttt
      601 ctattcctca atgatggtac atctattggc aatacattta gtctataata caggtcctct
      661 ctaaattttc cttctctaat catctttagt aagtttttgt ttgttgctgc tatgacccta
      721 acatctatgt ttataacatt atgggaacct ataggcataa cctgcttttc ttgtaatacc
      781 cttagtaact ttgtctgtag caatggaggc atatctccaa tttcatccag aaatatagtt
      841 ccattattag caagttcaaa tagacccttt tttccttctc ttaaagctcc tgtaaaagag
      901 cctttttcat atccaaacag ttgactttct aatagactat ctggaactgc tgcacaatta
      961 actgctataa acggttgatt acttctattg gaagcattat gaattgattg tgataataat
     1021 tctttaccag taccactttc tcctattatt aatacagaaa gttctgattt agatattttt
     1081 ttagccaatt ctacacactt taccatattt ttatttttag tttttatatc tttaaatgta
     1141 tatcttgcta tctgaccttg ttctcttaat ttattagtaa gattttgttc taattttttt
     1201 atatacgtta tttcttgtaa gttataatat atcccagctt tattccccat agaaaaaata
     1261 ctttttttat ttacatttat atatttttta tttgtaaata ccacttcatc aattatttct
     1321 ttgtcttcaa gtaaaatatt taaatcttca gaaatgatat cttctatgtt tttgccttgt
     1381 ggattttcat ttatacctaa aatttttaaa gcttctttat taaatgtgtt tattatacca
     1441 tctgctgaag taaatattat tccatctttt gatagattca atataacatc ttgttcttct
     1501 atttttaaaa acagttgcct ataattatct ttaactccat tatctaaact tattaaactc
     1561 tcagaatatg ccattaaatt tttacgtatt tctttaacat ctattctaag accatttatt
     1621 atttctataa aggtagatat atcaatatat ctatttccaa ggtcgattac tttatttata
     1681 taacttggta cttttccctt tactccaggc gttatagcta tgtctatatt tttataaatt
     1741 tttccttcaa tatatggagt aaactttaaa ttttttactc ctattttgta aaataagctt
     1801 atcgtttcta aggtagtttc ttttgtatca tttacaacca atacttcagt tccatttggt
     1861 attgaaaata atccatatat ttcattttct ttaagtgttc tccttatcac tataatctta
     1921 ctatcttgta ctaaatgctt tctcatactg tttaatctat catcattcat tactagtaca
     1981 aaatcacctt gtattttatc attctctttt aaaacttcca gataatagtg gtttatatca
     2041 atataattac ctaaaaccaa acgtatattt tcttctaaga aagatgatag tttagttatt
     2101 ttatcagtta ctatagctat tgattgtttc atatttatac ctcttattta tatagtatat
     2161 aattacttat atactacttt atttctacca agttgttttg ctttatataa atttttatca
     2221 gcttcttcta gtacatcttt gctatctgaa acatgagcta cacctgcact tatagtcaca
     2281 gtcacatcat cattccactt tttatttgca attgcaaccc ttatactgtc aattatatct
     2341 gctacttcat caccagattt atttcttatt aatacagaaa attcttctcc tccgtaccta
     2401 aagactttat catcttgttc taacataaca caaatggtat ttgttattcc ctttataaca
     2461 gtgtcaccaa atttatgtcc ataattatca tttatgagct tgaaattatc aatatctatc
     2521 atcacaagtg caaaattatc atctttctct ctgtactttt catacataat atcaaaactt
     2581 tttctattat aaacatttgt aagaccatca tgtaaatttt gtgctttaaa atacttcaat
     2641 cggttgtata aaaaagtaga ccctaatata gcaattaaaa ttactgtaaa taatatttta
     2701 taatatttta tttctttttt agttttttca aatagcttac tttcgtttga tactttatca
     2761 attatataga aactagtctc tgaatctctt atacttttgt tctctttata ttcatttatt
     2821 aacatctttt gatactcatt taaagattct atatttcctt gtttttcata tatatttaat
     2881 atcttattta aattttttat tttctcctgg gttaactttc ttttagtaga tatctctaaa
     2941 ctttttttat aattatctat tgcagaatta taattttcgc ttttatattc taagtttccc
     3001 attgccatat aatagtacat atctttatct aatatataaa ctgttctatc tgattttata
     3061 agataatcag ctttttctaa aaaagtcttt gccttttgta tattattttt gcccacatac
     3121 gcatttgact gcattataga ggccattata tcaaaatcat tgtaattatc cgtatttaca
     3181 aactttttat aatcttttat cctgctactt atttcaatag ctttatcata ttcgccctct
     3241 tctacatata tttctgctag atttataata gcatataatc taaccattcc atttttttca
     3301 ccttcatcaa tatctatttt aaaagaatcc tttattaatt tctctcctgt ttcatagcca
     3361 cctatcttta aaaatatgct cgccaaatca acatctagtc ttgctttttc ataattattc
     3421 cccaatttct cagccaactt tatagacttt acagttaaat ctactgcctt agaataatta
     3481 ttatccatta tatacactga tgctaatctt ttttctatat taaatctaca ttcatcagat
     3541 aatttcacat ctgattctct catcttttct aaagaatcaa tgactatctt cgttccatct
     3601 ggaacatatg aaatagtctt taacatatcc caaacttcat tatatacttt attgtatttt
     3661 ttttctggaa tagattcgaa agaagtctta accacttttt ttgcttcatc atactcctta
     3721 tctgccacaa ggctttcaga catatatttt cccttattta taatttctct ttctttcaat
     3781 ccattttggt catgctttat aaacaaaata atactaataa gtattataca tataaaaatt
     3841 atctttttgg gtaacctttt ctttggtagt ctaaaattca aaattttagt cctccttttg
     3901 tatattgtag aatgaaaact tacatatata aataacctct taataattaa taatacaata
     3961 tatcaaaatg atttacaaca tatgctagac ttaaatctta ataaaaaagt ctcctaaaat
     4021 aatttaggag actttaaatt taagctaata taaatttata cagttaaaac aagtgcttgt
     4081 tttttagttt tatatttaat atttatttat acatattttt ttagtactat tgtagcatta
     4141 tgcccaccaa atcctaaaga atttgatagt gcatatctaa tatttttttc tttaccttga
     4201 tttggaacat aatctaaatc caaatcttca ccaacttctt gtatgtttat agttggaggc
     4261 acaaaactat cttctatact catagcacaa acaacagcct ctatagcacc tgatgctcct
     4321 aataagtgac cagtcataga ttttgttgat gacacacata agtcatatgc cttttctcca
     4381 aatacagttt ttatagccat agtttcatat aaatcattgt aataagttga agttccatga
     4441 gcatttatat aatctacttc ttctaatgga acatttccat ctttcaaagc catattcata
     4501 gacctagctg ctccctctcc attttcagct ggagttgtca tatggtatgc atcagcagtt
     4561 aaaccatatc caactacttc tgcatatatt ttagcgcctc ttgctaaagc atgttctaag
     4621 tcttctaaga taacaatacc tgctccttct cccattacaa aaccatctct gtctttatca
     4681 aatggtcttg atgcagtttt agggtcatca tttctagttg acatagcctt catgttacag
     4741 aatccagcaa aagcaagacc tgttacagca gcttcagaac cccctgctac catgatgtct
     4801 gcatcacctc tttgaattac cttaaaagca tcacctattg aatgagttcc tgatgcacaa
     4861 gctgtacata cattagtgtt aggccctttt gctcctaaaa atattgaaac ttgaccagct
     4921 gccatatttc ctatcatcat aggtacaaaa agtgaactta ctcttttatt tcctttttcc
     4981 aaaagaattt tatgttgagt ttctatagct tcaactccac ctattccaga acctattata
     5041 acaccaaaac gttctctatc aactttatct aaatcaagat ttgcatcttc aactgctaat
     5101 ttagcagata ctattgcaaa ttgagcaaat ttatctaatc tcttcaattc ttttttactt
     5161 atatattctt ctggatgaaa attcttaact tctcctgcta tctgagtttg atatgtgcta
     5221 gcatcaaaat tagttatttt atctatacca gatactccag attttatatt actccagaac
     5281 tcttcttttc ctgttcccaa cggcgtaaca catccaagtc ctgttattac tactctttta
     5341 ttcatcggat acctccactt ttttaaagct cgactattta ttagcttcta tatacttaac
     5401 tatatctcct atagatttga agttttctgc ttctgtatct ggtatttcta taccaaattc
     5461 atcttcaagt gccattatta tttctactgc atctaatgaa tctgcttcta aatccttcat
     5521 taatgatgtt tccatagtta tttctttatc ttctactcct aattgctcta ttattatttc
     5581 tacaacttta ttaaacattg tgtacctcct aaaatttttt attttaatag atttctctac
     5641 tttttaaatt attattgcat taccattcca ccatctacat ttattacttg tcctgttata
     5701 taacttgaca tgtcactaac taaaaatgct acggcattgg ctacatcttc tggtttacca
     5761 taagatttct ttggtatgtt tgctagtgtt gattctttta catcatcact tagtacttta
     5821 gtcatatctg tttctataaa tccaggtgct actgcattta tatttatgtt tcttgatgct
     5881 aactctcttg cagaagcttt tgtaaatcca attacacctg cttttgatgc acaataatta
     5941 gtttgccctg cattacccat aattccaact actgaagtca tatttattat tcttccatac
     6001 ttctttttca tcataggttt agtaactgct tttgtacaat taaagacacc ttttaagttt
     6061 atatctataa ctctatcaaa atcttcttct ttcattctca taagcagacc atctttagtt
     6121 atgcctgcat tattaaccaa tatatcaatt tttccaaatt cgcttacaac agaatctatc
     6181 atttgattca cttcatcaaa tttagacaca tcacatttta tagaggtaca ctttaccccc
     6241 atactttcta tttcattttt agtttttagt gcttcatctt ctttagaagt ataattgatt
     6301 actacatcag ctccaaaaga tgctagtttt tttgctattt cttttcctat acccctagag
     6361 ccaccagtaa caactgctac ttgtcctgta agatttatca ttatatctct ccatttctat
     6421 aattttgtac aaattcatta agagaatcta taccatctat attataaata ttgacatttg
     6481 cttttttatt ggaagctatt ttcttaataa atgcttttaa agtttttcca ggtcccattt
     6541 ctataaaagt atcatatcca tcatttaaaa gtctttctat agaatcttcc caaagaacag
     6601 atgaacttac ttgttttata agcaggtctt taccttcgtt tttatagtaa tctgcattta
     6661 catttgccac cacatcaact ttaggtttag ttatgttaac tttttctaat tcactggcca
     6721 attttacacc agcttctctt agcattgatg agtgaaaagg tccacttaca tttaatttaa
     6781 cagctctttt tgctcctgct tctttgcatt tcaccattgc ttcttctatc ttttcatttt
     6841 caccagatat aactatttga ccaggagaat tataatttgc tacttccaca atacctcctt
     6901 gtatatcttt aactacattt tctaatatac ttctatctag acctaaaatt gctgccatag
     6961 ctccaacacc tgttggaact gtatcctgca taaacttacc tctttttttg accaagcata
     7021 cagcatcttt aaagtcaatt gcatctgctg caaccaatgc actatactct cctaaagata
     7081 aacctaaaca tgcactatat tttaaatcta cttgggcttg tactgctttt aatacagcta
     7141 cactatgtgt aaccatagca ggttgagtgt tttcagtttt agaaagttct tcttcatttc
     7201 cattaaacat aaggtttttt aaatccatat ttaaggcatc actagcctca tctattacac
     7261 ttttagcaac agtattattt tcatataaat ccttggccat acctacatat tgagctcctt
     7321 gaccagggaa tactagtgca acttttccca tcttgctcct cctgatttac agttctattg
     7381 ctggcataat ctctcttgct tgatttacca tagcctctat aatttcctta caaggtgtta
     7441 tttcttttac catagcagca ctttgacctg ccataaatga acctttttct atgtctccat
     7501 ctactgttgc aaatcttaaa gctcctgtac cttttttatc cagttcttca ggagtagctc
     7561 cattttgttc catctttaaa aattcttttg atagtttatt ttttaatgtt cttactggat
     7621 gaccagtact tcttcctgtt acaattgcat ctctatcttt tgcttttagt actaagtttt
     7681 tatagtttga atggacagaa cactcttcac tgcaaataaa tctagttcct acttgaactg
     7741 cactggcacc taatgcaaat gatgctgcaa ttcctctacc atctacaatt cctccagcag
     7801 ctattacagg tatgtttaca gcatcagcaa cttgtggaac taagaccata gtagtaagtt
     7861 ctcctatatg tcctccacct tcagtacctt ctgctattac tgctgtagct cctagctttt
     7921 ccattctttg tgccaaagct attgtaggta ctacaggaat aacctttgtt ccagcttcct
     7981 ttaatctatc catatacttt gcaggatttc cagcaccagt agtaataact tgaacttttt
     8041 cttctataat caaatcaatt atatcatcaa caaatggcga cataagcatt acatttactc
     8101 caaaaggttt atctgttaat tttttacact caacaatttc tttctttata gcttcttttg
     8161 gtgcgtttcc tgctgctatt atgccaagtc ctcctgcatt agatacagca cttgctaatg
     8221 atgcagtagc tacccatgcc attccccctt ggataactgg gtattttata tttaatattt
     8281 tgcaaatttt attcatacaa ttctccttca cttgaaatca taaaagtaaa ctttttatta
     8341 ttaatcaact ttattttgtg tataagttta attttgttaa ttaaatcatt ttagatattt
     8401 tatactataa agtccacttg acaactgaag ccccccaagt tagacctcca ccaaagccaa
     8461 ctaaaactac attatcaccc tttttaattt taccttctct ataagcttca tctaatgcta
     8521 ctggtataga tgcagcagac atatttccat atttgtctat atttacataa acttttttca
     8581 tatcaagctc taatctctta gaagaagctt gtatgattct tatattagct tgatgtggca
     8641 ctaagtagtc tatatcagtt gtatttagat ttgctttttc aagagctctt aaagaagttt
     8701 ctgccatctt tctaactgca aatttaaaca catcactacc tgccatttct attgtatgta
     8761 gattagcttc tacagtctct ttacttgcct taagtcttga acctccagca ggtactttta
     8821 aacaatcttt tcctttacca tcagagccca tatgtgtaga cagtattcca ccctcattta
     8881 cagggccaat aacagctgct ccagctccat ctccaaacaa tactgctgtt gttctatcat
     8941 cataatttaa aaacttagaa caagtttctg ctcctattac aagcacattt ttatagcaac
     9001 ctgtttccac aaattgcttt gcaatagtta ctccatatac aaatccagaa caagctgctg
     9061 atatatcaaa tgcagtagca tttactgctc ctattgcatc ttgtactata catgcagttg
     9121 aaggcaaaga catatcagga gtaattgtag ctaatataat cagctctata tcctctacgt
     9181 ttaactttgc acattctata gcttttaagg cagctttaga tgctaagtca gaagttgctt
     9241 cagactcttt ggcaaatctt ctttccttaa taccagttct agtacttatc cactcatctg
     9301 atgtatccat tattttttcg aagtgaaaat tatcatatga ttgctcaggt agataacttc
     9361 ctactcctaa tattccagcc tttgtattca ttatatatta aacctccaaa acgagtttat
     9421 tcatcttcgt tatttttatt ttcttcattg tatttgctta taaattggtt aatatcttca
     9481 acgacttttc cctttgaaaa ttttattcct tgatttatag cattttttat agctttagca
     9541 tcagaactac catgagcttt tataactcct ccatttagac ctaaaagtgg agctccccca
     9601 tactcagaat aatctataaa actttttaat tttcttaaat catcctttat aagtaatgct
     9661 cctattttac tttttgtact ggccataaaa gtttccttta taagactcat aactgaaagt
     9721 gctacacctt cagcagattt aagtaatata ttacctgtaa atccatcaca tatgattata
     9781 tctgtgtatg catttataac ttctctagct tctacattcc ctataaaatt taaatctaat
     9841 tttttaattt cttcatatga ccttttgact aaatcattgc ctttgccttc ttctagtcca
     9901 acatttgcta atgctacctt aggattttca agacctaaaa cttttcttga ataaatatta
     9961 ctcatagctg caaattcaac tagattctta ggtttacaat ctgcatttgc gccacagtct
    10021 gctataagtg tcattcctcg ctttacattt ggtattgctg aacataagca aggtctatcg
    10081 attcctttta ttctaccaac aacaaataat ccaccagcaa gtaaggctcc tgtatttcca
    10141 gctgatatta tagcatctgc ctttccttct tttacaagat ttaatgctac taccatagat
    10201 gaatcttttt tacttcttat agcctttact ggtttatctt cgttttctat aatctcagta
    10261 gtatgaacta tctctaattt atttctatca aattcataat ttgaaaattc cttttcaagt
    10321 aaatctttat ctcctgtgat tattaaatca acctgatatt cttttatagc atttactgcg
    10381 ccttctacat tagatttagg tgcattatct ccacccattc catcaattac tattttcata
    10441 atttccctcc taatctaatt cttcaaagat aaactttcct ctaaatattt gctcttgggc
    10501 tttattgttg ataacaactt ttacataatg ctttttatct atatttctat acacttctgc
    10561 ttttgctata agtctatctt gttgttctat caacttaaaa ctttttacat ttgcaactct
    10621 cataataacc ttaggtgcat ctattatagc cattgccaat gaatctgcta gagaaaaaat
    10681 atacgtatct tttaaagtat tagtcttaga gtaaatcatg tcctgtgtaa cttctaacat
    10741 agatatacct aatcgcccta cagataaatc aatgatttct cctgtaattt cctttactcc
    10801 taaagtcttt acttttgaac tctgagtttc tgctattgat ttgactcttt ctcttagctc
    10861 tggtatgttt agactcattc tatctaatct tatggtctgt atacttacat caaacagact
    10921 tgataattct tcatcagtat aaaaaggatc tgtctttagc atatctataa gctctttttg
    10981 tctttgggct ttacttttct ttttcatgtt ccctccaaat tttatagctg taattagtac
    11041 cagctactaa tagtagtata tagtaacaca caaaaatttt caatacttat attattattt
    11101 ttctattttt taatatgcat aaaatccact tattttctct agtaaatcat cttatttttt
    11161 tatatgtaca aaaaaaacat gtagagtata ctctacatgc tttttagaag ctattactta
    11221 gacacaactt ctttaccttt ataatatcca cagtctggac acactctatg tggtaatttt
    11281 ggctcatgac attgtgggca acttacaaat cctgttgctt ccatctttga gttagctgct
    11341 cttctcattt ttgtgtttga tttagacgtt ttacgctttg gtactgccat ttacgccacc
    11401 tccttagtca aaatttaatt aaataaatct tttaatttag caaaacgagg atctatgtat
    11461 tcctcatcgt ttgtaatttc actgcaagaa cactcttctt catttaagtt tgccccgcat
    11521 acttgacaaa gacctttaca gttttcacta caaagaactt tatgaggtac attaaaatct
    11581 aatgtctgtt ctattatatc aataaggtcg atttcttcac catcgaatat aaaaggatca
    11641 aattcctcaa actcatcttc atcataatct tcttctttta ctaaaaaacc ttgtatagaa
    11701 tactctagtg gtatttctac ttctttaaga catcttgaac aattgtcaac aattgtaaaa
    11761 ttgacatcta tatctagata caaaccttta ttagtctttg acactttccc ttttaaattt
    11821 acaggtgata ctaatttgta aatctcatca caataactaa tagtatcaat tttttcacaa
    11881 aaattcaagt ctattttgtc agtttctttt ctatttattt tttctatgct aactttcatt
    11941 aagttcacct caacatatca ccaaatttat tatacaaatc tagcatttgt ttgtcaagta
    12001 tttttacttg ataggcatag gtttaattta attattttac caatcttaat gtatctctag
    12061 ctatcattaa ttcttcatta gttggtatta aaagcatttt tactttagaa tcagcagtag
    12121 atattactgt ttcttttcct ctaacatcat tagcttcttt atctaatttc attcctaaaa
    12181 actccatgtt agaagcaata tctgctctta tagcctttcc attttcacca acgcctgcag
    12241 taaatactac aacatccaat ccattcattt cagcagcata agcgcctata tatttttgta
    12301 cttttttaac atacgcttct aatgtagctt gagctctttc gtctccattt ttagctgcat
    12361 cttctatatc tctgaagtca ctagaaattc cagtcattcc atatactcca gattctttat
    12421 tcattaattt atccaatcca tcagcatcta aaccttcttt tctcattaag aatggtaata
    12481 tagctgggtc tatatctcca catctagttc ccattattaa tccttctaat ggagtgaatc
    12541 ccatagatgt atctacacat tttccaccat caacagcagc tatagaagca ccatttccta
    12601 agtgacaagt tactattttt aaatctttta tatcttttcc taatatctct gctgctctct
    12661 gagatacata attatgagaa gttccgtgga atccatatct tcttacccca tatttagtat
    12721 atagctcatg aggtaaacca tataagtatg aagatttagg cattgtttga tggaaagcag
    12781 tatcaaatac acctaccatt ggtacatctg gaagtatagc tttacaagca tctattccca
    12841 ttatgtttgc tggattatga agtggagcta aatctataca ttcttttaaa gcttcttcta
    12901 cttctggagt tattactact gaagttgcaa atttttctcc agcatgtaca actctatgtc
    12961 ctactgcatc tatttctttc atttctttaa ctgcaccaaa ttctggatga gcaacagctt
    13021 ctaaaactaa tgctattgca tctttgtggt ccttcattgg ttgttcaaca acgtatttat
    13081 catctctacc tgctttttca tgttttaata tagaaccttc tattcctatt ctttcaacca
    13141 atcctataca taaaacttct tcattattca tatctattaa ttgatatttt aatgaagaac
    13201 tcccacagtt taatactaat attttcatta ttatttcctc cttagatttt tttataaata
    13261 atatgtaata aattttgatt tgtaataaat gtctaaataa aactctatct tttattattt
    13321 tgttatagat atattattgt atacattaaa cgattttcat aaataatata tcacctttta
    13381 atataa
//
LOCUS       DBGJFU010000105        13328 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.105, whole genome shotgun sequence.
ACCESSION   DBGJFU010000105 DBGJFU010000000
VERSION     DBGJFU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.105"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            139..768
                     /locus_tag="ACOIAS_002826"
     CDS             139..768
                     /locus_tag="ACOIAS_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2702527.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMYSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            743..1147
                     /locus_tag="ACOIAS_002827"
     CDS             743..1147
                     /locus_tag="ACOIAS_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HGZ2702528.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            1150..1623
                     /locus_tag="ACOIAS_002828"
     CDS             1150..1623
                     /locus_tag="ACOIAS_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HGZ2702529.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            2193..2858
                     /locus_tag="ACOIAS_002829"
     CDS             2193..2858
                     /locus_tag="ACOIAS_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HGZ2702530.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            2880..3812
                     /locus_tag="ACOIAS_002830"
     CDS             2880..3812
                     /locus_tag="ACOIAS_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HGZ2702531.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            3833..4474
                     /gene="pcp"
                     /locus_tag="ACOIAS_002831"
     CDS             3833..4474
                     /gene="pcp"
                     /locus_tag="ACOIAS_002831"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGZ2702532.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(4594..4926)
                     /locus_tag="ACOIAS_002832"
     CDS             complement(4594..4926)
                     /locus_tag="ACOIAS_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2702533.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            5192..5812
                     /locus_tag="ACOIAS_002833"
     CDS             5192..5812
                     /locus_tag="ACOIAS_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2702534.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNKLIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(6137..6961)
                     /locus_tag="ACOIAS_002834"
     CDS             complement(6137..6961)
                     /locus_tag="ACOIAS_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HGZ2702535.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(6972..8375)
                     /locus_tag="ACOIAS_002835"
     CDS             complement(6972..8375)
                     /locus_tag="ACOIAS_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2702536.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(8379..9104)
                     /locus_tag="ACOIAS_002836"
     CDS             complement(8379..9104)
                     /locus_tag="ACOIAS_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702537.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLFAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(9216..9989)
                     /locus_tag="ACOIAS_002837"
     CDS             complement(9216..9989)
                     /locus_tag="ACOIAS_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702538.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(9995..10882)
                     /locus_tag="ACOIAS_002838"
     CDS             complement(9995..10882)
                     /locus_tag="ACOIAS_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702539.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRAGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(10896..11918)
                     /locus_tag="ACOIAS_002839"
     CDS             complement(10896..11918)
                     /locus_tag="ACOIAS_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702540.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALN
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            12493..12669
                     /locus_tag="ACOIAS_002840"
     CDS             12493..12669
                     /locus_tag="ACOIAS_002840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702541.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         4901 a         1909 c         1531 g         4987 t
ORIGIN      
        1 tacaattaat aacattaatt tattatccat aaaaatatat taacttatat tatttaataa
       61 tttattgaca gtattaaaat taaattatac aataacagtg taaattaact taacaccgtt
      121 aagcaaagag gtgattatat gagtatcaaa gaaagacgta aaaatgaaaa agaagaaatg
      181 aagaaaaaga ttatggatgc tagcatcgaa attataaacc aacatggata tgaaaattta
      241 tcaataagaa aaattgctac aaaaatagaa tattctccaa ctacaattta tctttactac
      301 aaagacaaag cagagattat cagcgatatg accaataaac tttataacac agtagaaagt
      361 aatgccattg acattatgaa taattgctca tcactaccaa tagataaaca aatcatagaa
      421 attatgacta gctttataaa aactctttct agtgaaccag agatggcaaa atctatcatg
      481 tatagtagaa tgaatattat atttgcatct gaaaatacaa acaatacgcc tagcaataat
      541 ggtataaaaa tgcttgataa attcctttcc attggaattg aacaaggaat attcaaaaaa
      601 aatatagata aaagctcatg gatgcttata agtgcattgt taggattcgt tttatgtgtt
      661 gttgaaaata aactatattc tttacataat ttttctcaac ttatagaaaa tttcttagat
      721 attttagtgg gaggacttta caatgaaaat tcgcaacaag gctactaata aaaagaaaaa
      781 atatgtaaaa atatctattg ctctattctt tgtatttata tgcattatta ttgtaattgt
      841 aagcaactta aagccagaaa acttggtagt aaaagatata gatattaata atgttaaaaa
      901 tggcatttat actggaagtg ctgataataa cttggtaaaa gctactgtgt ctgttgaagt
      961 taataatggt aaaatccaaa atattaatat cttaaaacac gaccatcttt taggtaaacc
     1021 tgctgaaaaa attacaacaa gtattattaa acaacagtct ttagatgtgg atgctataac
     1081 ttctgcaact tatagcagta atactatacg aaaagctgta gaaaatgctt taagaaaggg
     1141 agaataatta tgaatactat tattatatat tcaagtaaat atggttgtac aaaagactgt
     1201 gcaaatatac taaaaaataa actatcagat aatgtcacta ttgttgatat taataataat
     1261 aataaaattg agttatcaaa atttgataaa attattattg gaagttctat atatgttggt
     1321 tcagtttcaa aaaaaataca agtgctatgt aatgataatg tagagttatt aaacaaaaaa
     1381 caagttggca ttttcctttg ctgtggcttt tctgaacaag cagataaata tttaaaatca
     1441 aattttccat catccttatt agaaagtgca aatgctattg gaatttttgg tagtgaagct
     1501 cgattagaaa aaatgaaatt tttagataaa cttattatga aggctgtttc aaaaggtaat
     1561 tatgacagtt ttagaatatc acaggataat atagataatt ttttaataaa tcttaatagc
     1621 taaaataaaa ctaattattt ctatgataaa ttcttttatt tataagtaat attgctcaaa
     1681 atagtcttta aactttatca aaattttatt tttcttagtg aaatctaatg aaactagcac
     1741 ttaatatatg ctttattaat cataacaaat atgttttatt aatcataaca aaaagagagt
     1801 gtctaatgaa ctaaaaaatt cattagacac tctctttttg tatgaaatca aatatatttt
     1861 tatcatttca aaagtgaaaa atacacttgt ctctatgttt ggcacattct ctttttataa
     1921 tataatatct aatttaaaac ctatttcaaa tattgacata tacttaaact atagacttac
     1981 aaaatcaatt catattagct taaaatataa tctttgttat tcaaattcct atacacaacc
     2041 attatttact ttgatacttt aaaacatctt ccctattttt tattaaattt tataataata
     2101 gtcctattaa cgcaaatact ataaaaagag tttaacttta tccattaaat aaatttaaat
     2161 taaaacatat agaaatggaa ggagaacaat ttatgataaa actaatcggt atattgatag
     2221 ttgtaattgg atttatactt aaaattgaca cattatttac tgtattatta gcaggagtgg
     2281 caacaggaat tgttgcagga ttagatttta atcaaatttt tacaatttta ggagattctt
     2341 ttgtctcaaa cagaggtgta tcattattca tattaacatt gccagtaatc ggtgtattag
     2401 aaagatatgg tttaaaacaa agagctgttt ctttaataga aaaattaaaa agacttacaa
     2461 caggaaaagt acttactata tacatgatag ctagacaaat agctggtgct ctatcaataa
     2521 gaatgagtgg acatcctcaa tttgtaagac cacttgttaa tcctatggca caagctgctg
     2581 gtttaagtaa tagtgatgaa ctaaaagaat cggatgaaga agccataaag gcattatctg
     2641 ctgcatcaga aaactatgga aatttttatg gtcaaaactt atttgcaggt agtagtggag
     2701 tattacttat agcatctact ctgactcaat ttggatacaa tgtaactgga cttaatatcg
     2761 taaaggctag tactattatg gcagtaatag cacttgtagt ggctacaatc cagtttacct
     2821 tatatgataa aaaattaaac aaaagtcata aaaaatagtt ttatttagga ggaataaaaa
     2881 tgcaaaattt tataaacata tcattagaaa tcttttacgc actaatggga tttttaatga
     2941 ttgttatagc atataagtct tttactacca taaataacaa taagaaatat ggtacatcat
     3001 tattttggat acttatatca ctgccattca tatttggtag attgattcca gccaatataa
     3061 taggaataat attaatatta tctagtttat taacactttc taaacaagtt gtatttgcaa
     3121 agtatgaaga accagatgaa aattttggaa aagaacaagc agacaaactt aaaaataaaa
     3181 tatttatacc atcattaata ttagcatttg ctgcagtagt agtagctatg agcctttcta
     3241 attttgataa ttcttctcaa tttgcaatag gtgtgggttc aataattgca ttaatatcag
     3301 cccttataat aactaaggca aaacctgcta catcagttca agatggctca agattacttc
     3361 aacaaatggg acctgctagt atgttaccac aacttttagt tgcattggga gctttattta
     3421 ctcaagccgg agtcggtgaa gttatttcaa ccatgatatc tggtgtagtc cctgctgata
     3481 gcagattatt tggtgttata gcttatgtat taggtatggt tatatttacc atgataatgg
     3541 gaaatgcatt tgccgcattt tcagtcataa cggcaggaat tggtattcct tttgtattgt
     3601 ctcaaggtgg aaatcctgct ataattggag cattagcatt aacagcagga tattgtggaa
     3661 ctctacttac tccaatggct gctaatttta atatagttcc agcagccttg ctagaatgta
     3721 aaaatgatta tattgttata aaatatcaag ctccagttgc tttggtatta attatagccc
     3781 acatattagt aatgtacttt ttaggatttt aatataatta ggaggtaaaa taatgaaaat
     3841 tttgttaaca ggatttgacc catttggtgg agaaccaata aacccagctc aagaagcagt
     3901 tgaaagggtc aataataata taaacggtgc agaaatcata aagataacta taccaacagt
     3961 tatgacaaaa tctgttgaag ctatagataa agctattcaa gagcacaatc cagatatagt
     4021 aatatcagtt ggacaagctg gaggtagatt tgacattaca cctgagagag ttgctataaa
     4081 tatagatgat tttagaataa aagataatga gggtaaccag gttatagata ctattataaa
     4141 agaagatggt gaacctgcat atttttcaaa attgcctgta aaagctatgg taaaacatat
     4201 gaatgaaaat aaaattccag ctagtgtttc aaacactgca ggaacatttg tttgcaatca
     4261 tgtgatgtat ggaattcttt atatgataga caagaaatat cctaatataa ggggtggatt
     4321 tatacacatc ccatatacaa cttcacaagt aatagataaa aaaaatacac cattcatgtc
     4381 tttagaagaa atagtaaaag gcttagaatt agctatagaa gcttgtatta tatataaaga
     4441 agatgtaaaa gaaattggtg gtgaaatttc ataataaatt ttttagtatt tttataaatt
     4501 aaaaatacta actattaatt taaatataga ctgtgagtag attattcaaa caattttact
     4561 cacagtcttt ttatggtatc tacagataca aaattaaggt ctattcctct ctccccactc
     4621 acacattgca tctaaaattg gaatcagaga ttttcccctt tctgatagac tatattctac
     4681 cttaggaggt atttgaggat attcagtacg aataatcaag ttatctgctt ctaattcttt
     4741 taatgataaa ctcaaagttt tataggagat agtatttata taacgtttta atgcattata
     4801 tcttacaact tcaaattcta caagacagta aagaattatc atcttatatt taccactaat
     4861 cagtgaaagt gtataactaa atcctgtttc acttaaatct gtatttgata tacagtctat
     4921 actcatatat tacactctcc ttttggttag tattgaacaa aaatgtgcgt acttgttttt
     4981 agaataattt ataataaaat tatagtgtaa gaataattga aagtcaattt tataatagga
     5041 ggtgtattta ctaaaaaaaa agttatttta aataggattc taagattaga catttctata
     5101 taaattgata atattcatga aaatcctatt ttataaaaaa taatatctct tagaaaatct
     5161 attaaataaa attcattgga ggaaatttat aatgaaagtt ttattgataa acggtagccc
     5221 aaaccaatat ggatgtactt atacagcact aaatgagatt actaaagtat tgagtaaaca
     5281 cgatattaaa acagaaatac tgtatttagg taaagaaaca atcccaggat gtattagttg
     5341 cgcatcctgt tttgaaactg gaaaatgtat tcgaaatgat aaagttaata aattgattga
     5401 agatctggat aatattgatg gtattattat tggttctcct gtttactttt ctagtgcaac
     5461 tggtcaatta acttcttttc ttgaccgtct atttttcata gctggaagtc gcatggctac
     5521 aaaattaggt gcttcagttg tgtcatgtag acgtggtggt gcaagtgcaa catttgacca
     5581 actaaataaa tatttttcaa tcagcaatat gcctattgta tcatcacagt attggaacca
     5641 ggtacatggc tttactccag aggatgttat gaaggatgaa gaaggattac agactatgcg
     5701 aactctagga gaaaacatgg catggcttct taagtgtata tcagcaggta aaaaagctgg
     5761 aattaaagaa ccacaatatg aagaaacaat tatgactaat tttataaaat aaataaaaaa
     5821 tgtactaata ttaattaaaa caaaacacta atttataaat aaaataagca taaatgtagg
     5881 ataaaaagca ctaaaacaga ggtattgaat tttatatcaa acctctgttt tattattaca
     5941 ttaataacca aaccatgtat aacatcatat atttctaaat aaattataat attatattct
     6001 aatgttattg aaattacaat aactgaattt ctgctcataa atttaaatat tctacaaatc
     6061 tcatctaaat aacaatttta atagacctaa aacaatcctc atactgttca ttttgtatta
     6121 aaaatatcac ttacaatcaa aaaccattta ttaagtatat tcctaagata tacaatatat
     6181 atacatggat tggatagtat aaataaaata aatacttcat actcttccct ttttctccat
     6241 tgtaaagtaa cattaaaggt agagaagcaa tcatcatcca ttggtaattt tgtaaaatag
     6301 aattttttaa actataatct ccatttgaca ttgttgtaaa tataaatata cacattaaaa
     6361 cataacaaat actcaaactg aactttttac ctctacacaa ataaaaaata atcccaagta
     6421 taatccaaat tggtccacct tctactaaaa atggtactgg taccaaaatc ataaataaag
     6481 caataactct gttagtgact ttaaaaagta aagctactgt aaatattcct aaaattatag
     6541 gaatgattat catcaataat cctataataa aatatttaat ttctttctct tttctaaatt
     6601 tctttatgaa ctctattgct tgtaaataaa tcgctattaa aaataatgtt gaaaaaatgt
     6661 tatttataat tatagctcct tctggatgtg aaaatacatt attaattatt tgatttccta
     6721 tagccataat aacagaccaa atatacaatc tacttatata tttttttctg ttactagtgt
     6781 gataaaatcc ttcaacagcc atgaatataa acagtggtgc tgatattctg cctacccaat
     6841 gaaaccaaat tggtatttgc attgtttgtg gcataaatgc tgctatatgg tcaaatgtca
     6901 taaatattaa tgctaaaatt ttaatagtaa acccacttat acctttttta aaaaaatcca
     6961 tatcatatct cctaattttg ttttatctct ataataattt ttgttcctat tccaatttca
     7021 ctacttacat aaattttacc tttatgaatc tctattattt ctttagaaat agccatacca
     7081 agacctgaac ctttatgcat ctcacctgta ttagtacctc tataatattt tttaaaaata
     7141 tgtttcaaat cacttttgct tattccaatt ccattatctt ttattataat ttctatatta
     7201 tcttttttta caatttctac acttatcaaa gttccttcgc tattatgaac tattgaatta
     7261 aatataagat tggttatagc ccttctgaat aagattgaat ctacatatac ttcaattaaa
     7321 tcttcattac tttcaaattc aatattccta tttttatatc tattatcatt taaaatatca
     7381 attattatat ctctgactaa actgactaaa tttatttttt tcttgtccag aactatagta
     7441 ttatttttta atcgagtaga taaatttaaa tcatcaacta aatcttttat atacttagac
     7501 ttattatata taatttctgt atactcttgt atttcatctt cttcaaactc ataatctttg
     7561 tcattaatta tctctgcata cccttgtata gatgctagag gtgtctttat atcatgtgtt
     7621 atatttgcca gccattcctc tctcaactct tcattttctt ttctctcaac ttcatttact
     7681 cttaaagtat cagctaacat atttatattt ttaaaaactt cttcatatat acctctgtcc
     7741 ttaagatata aactataatt tccattagcc atagtctcaa tactccatag tacgcttgaa
     7801 attggttttg taagttttct actaaataaa tatcctacac ctaaagctac cacgctatca
     7861 attactagtg ttataatgat aactttattt aaagtttttt taatgttatt ttgagaatag
     7921 gttaaaatta ttcttgatat atctctacta ggtataccta ctattatgtt taaatgtttt
     7981 ttgtttaaat atttttctag tacaaaatta acatactttg tttctatata cttgtaatta
     8041 ttcaccattt gaaagggtgt atattttttt ggagtatcct ttggcacatt tactccataa
     8101 acttcctctc ctaaatcatt taaaacctgt atccaggaat ttgatttttc taatttttca
     8161 gctccaatct tagatagttt aaattctcca tctttttcat ataaatcttt ttcaaaagaa
     8221 cgtgcaaatt cttcaggatt atttccagaa tctacaactt taaaaaagct attagttgaa
     8281 atcacataca atatagatat gatatttata attactactg atatagctac aaacacaatt
     8341 gtaaatataa aatttctagt aatcttccac ttcatattct aatcttcctt tactgctaat
     8401 ttatatccaa gaccttttat tgtcagaata tattcaggtt ttgaaggatt atcctctaat
     8461 ttttccctaa tttttcttat atgaaccatt atagtattgt caaatccaac ataatcttct
     8521 ccccacactt tatcccatag tttttcttta cttataattt gattttgatt atgtgcaaat
     8581 aaagatagga tttttagttc ctttgcaaac agtcctatag attttccatc ttttataagt
     8641 tctgccctac tttcatttaa tatatatggg ccaaatgata taattttttc ttcattatta
     8701 tttagatttt tactttcgtt ttgagcatca ctaaaataac ttaatcttct caaatgcgct
     8761 ttaactctaa aagccacttc cttagggcta aaaggttttg taacataatc atctcctcca
     8821 atcgctagtc caagtaactt atctaattct tcggttttag ctgataaaaa tattattgga
     8881 gaattggagg tttttcttat ttctttacaa atatcataac cttcgccatc tggtaacata
     8941 atatccaata caatcaaatc aggattaata cttttaaata attctaatcc ttctttttta
     9001 gtttttgcag tatatatatt attaaggcct tcttttttta ggacagtttc taaaagcttc
     9061 aatatttcta cttcatcatc tattattaaa acacgatttt ccataaacaa cctcctgtaa
     9121 taatttacat atatattagc atatatttta tctaaaattc atttatctta tttttaatta
     9181 aactaaaata taaataaaat tactattatt tttagttaaa accttttgct tgatgtaaaa
     9241 ttttcatatg ttattttagt taacactatc attaatataa ttgagattac tatagaaaaa
     9301 actatcaaaa tacttggtac attatactta accatttgtt ggctcatcat aatccaattt
     9361 ggagtcactg acataaattt tatagcttta tctggtataa aacctacact aaagtatgga
     9421 attgccatta tcaatccacc tagtataaat ggaattacag ccgatttaaa tatagatgaa
     9481 agtgttaagc ctatttgtgc aaatataaaa gaagcaaaaa tagtagtcaa tataacaatt
     9541 atatacattt gtattactgt aagatttaaa ttactatttc caaagtacca taagttatga
     9601 atcgattcat ttaaattttt aaaatcaaaa aaactaaaac ctctaattcc tactaacaat
     9661 ataaaagaaa tattaataat agataaaatt aatccattag ctactagttt tgataatact
     9721 actgattttt ttcccttttt agttgtattt attaaacctg tcatattaga atcatattct
     9781 ttaatgtaat ttccagaagt tattataaac attatcaaac ctattagagg tattgagcct
     9841 tctatcttgt aaaatatatc tgcataagta tctaaaacat caaactttct aatcagaaaa
     9901 aatccaaaaa atattaaaaa cagccaaatt atccaaacac tctttgattt taatattttt
     9961 atcatttcag ctttaactaa atacatcatt tctactacac ctcccttgca tcttctaaat
    10021 caaaataaaa catgtataca tcttcgaatt ttgcttgaac tggttgtata ttatcctcgc
    10081 ttggaatatc ctctgaaaca attctaagta ctattgaatt aatatcactt tgaatactta
    10141 caaccttata tttagattgt atatcatcaa cttcacattc atttttaact tgaacattat
    10201 atactttatt ttccatgtga cttaatatgt ctttgtgtgt tcctttcatt aggagtttgc
    10261 catctttaat cattattgtt tcctttgcaa ctgattctat atcggaaatt atatgagtag
    10321 aaagtattat tatcttatct ctagacattt ttgctattaa attcctaaat ctagctcttt
    10381 cttgtgggtc aagacctgct gttggctcat caaggactat tatcttaggg tcgtttagta
    10441 aaacttgtgc tatcccagct ctcctcttca tacctcctga aaattttcct atagacttat
    10501 ttctcacatc ataaagacct actaaatcta ataattctcc tatcttacta tttaaatatt
    10561 ttccccttaa accttttaaa gctccaatat actgtaaaaa ttcttttgct gtaaaatttt
    10621 tataaactcc aaattcttgt ggcaaatatc ctaaaatatt tctataatca gcatccattt
    10681 taaatatatc ctcaccatta taaagtatct gaccttcatc aggttttatc aacgttgtta
    10741 tttgtttcat taatgtagat tttcctgctc catttggtcc tagaagacca taaactccat
    10801 tttctagagt tactgtaatc ttgtcgtttg ctactttttt accatatatc tttgaaagat
    10861 tttctatttc taatttattc atcttaactc cccttttatc tacttatata atctgatttt
    10921 attactaaaa aacataatga catagcacct atcaatattg ccatcaaaag tcctattgct
    10981 atattgacaa tgtctatatt cactttaaat atcataatat cacctagata atttcctatt
    11041 attttattaa aaccaaatat caaatcataa taatttacat ttgatacaac tgaaagtaat
    11101 tttttaggta atatatggat taccgacatt atcttagtta tagatataat aaatataaaa
    11161 aaacttattg aagatattga agacttactt ataaaagacc caaatatggc tatcatggcc
    11221 catccttcaa acatcaaaag ctctatgcct atatttaata ttacatactt tgttatactt
    11281 aaattagttt tcaatgcaac tacattgtct actattctaa aagattctaa gtctccatta
    11341 acaggtactc cttgttgcat acaagttata ataaatattc ccattaaata aacagaataa
    11401 attactaatg gaactaataa agcaacaaat attttagaat ttaatgcttt tttcttctct
    11461 tttgatgata gtatgatatc ttttaccgaa gtaacgactt catctacgta aatattagat
    11521 ataattatag atataatgat taacattcct acatttataa gtgaatttgt aactctatag
    11581 ttaaatactt tccaaaaact aatatcctta tattcattca cacttagatt atcaagtttt
    11641 ttctttgaca ttaattcaat ttttttagaa aattcacctg tatctgtttg atttgaaaac
    11701 tgctttaaaa cattaatctt attttgaaat ttctcattac ctatgtcgat accacttctc
    11761 gtatcttcta tacgtccttt ttctttatct atataataac tttctgtttc taaaacaggc
    11821 gtaataaaga tactaatcat tagtattaac actaatatgc ctagagatat aaacatactc
    11881 ttagatttag ccaatttttt tatttcccaa tttaccaata tatttctcct cctaaatcat
    11941 tttaattaca catatatatt aaaacttaaa tcttatataa atataatact aaccttaaat
    12001 aaagcttaaa ttattttata atttataggt gtgtttatta ttggagttac atttattatt
    12061 tttctgggaa attatacatt ttcaattatt tttattattt ttgatatttt tattgattgg
    12121 aaattttatt gttgttcaaa ttgtagttat tctttaaatt taaagatgga gttttttaag
    12181 aatagaacct actgtcctta ttatggaaag attattaaat agttgcatat aactttattt
    12241 atcttgaatc ttatttttaa aaacaattat aattttagaa tattttctat tctcaaacaa
    12301 gcatgttgaa taatgaagca tcttctagta ttatatactt gtagaataaa actaaattgg
    12361 taaaactggg aatatttaac cttaaggagt taagttatag aaactagtac taaaaaattt
    12421 tataaattat gttaattgac taatagaatg cgacttttta tttttactaa attttttagg
    12481 gggatggata atatgtttat tggagaaata gacaaatgta cacatatact aactgcttac
    12541 atcagtagtt catatgatta ttgtaatttt ttagatacac agttagatga ttttatatca
    12601 gaatatggag aaacggtagt tgaaacctgc ttacatcaag tcttattatt agtaagtaga
    12661 tacaattaaa actaaatttt tctagaaaaa cctttttaga tttatctact ctaataaata
    12721 tataattttt tgtactttat aagtaactat aacttctgct aaagtaaaga tgtattggga
    12781 gtacatcttt actttcccat tatagaagtt aaaagcatac accataaaat taatcatcca
    12841 atacaaatta tatatttcct atgtctaact taatttacaa gaatcattct atatgcatat
    12901 tctgcaatcc actaaacatg gctctaaacc tttactattt tatacattct ctataaatca
    12961 tacaaatcat atataaaatc cccctaatat gtttaaacca cctataaaat aacaccaata
    13021 tttagaatat tcaatatcaa ctgcataact ggtcaataaa aacgtataac tagacaaatt
    13081 tcaatattta tataaataat acaagaaaaa caagcatttt atttatataa gttttttatt
    13141 ttttgtttat attaaaaaac tatattgatt aatttatttt atttagtaat tataaaaatt
    13201 cgtcttagaa tatattctat atttgtagat tagctttaat atttttaatt tcacaaaaat
    13261 taaactccca atatttttat ccacttttat ctcattaatt aattatatta tttttatgct
    13321 ttttacaa
//
LOCUS       DBGJFU010000106        13129 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.106, whole genome shotgun sequence.
ACCESSION   DBGJFU010000106 DBGJFU010000000
VERSION     DBGJFU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.106"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            102..1439
                     /locus_tag="ACOIAS_002841"
     CDS             102..1439
                     /locus_tag="ACOIAS_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HGZ2702542.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMIFYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(1775..2344)
                     /locus_tag="ACOIAS_002842"
     CDS             complement(1775..2344)
                     /locus_tag="ACOIAS_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HGZ2702543.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYFNRYN"
     gene            complement(2700..3713)
                     /locus_tag="ACOIAS_002843"
     CDS             complement(2700..3713)
                     /locus_tag="ACOIAS_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2702544.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPSDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            complement(4325..5227)
                     /locus_tag="ACOIAS_002844"
     CDS             complement(4325..5227)
                     /locus_tag="ACOIAS_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGZ2702545.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            complement(5354..5473)
                     /locus_tag="ACOIAS_002845"
                     /pseudo
     CDS             complement(5354..5473)
                     /locus_tag="ACOIAS_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(6016..6609)
                     /locus_tag="ACOIAS_002846"
     CDS             complement(6016..6609)
                     /locus_tag="ACOIAS_002846"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGZ2702546.1"
                     /translation="MVGAVAGLIVLLYRILLGQAGTWLSQILKFVEGSTIKIIAWFAV
                     LAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKIKQVWWRVLPTKFIGGFLSLMGGLA
                     LGREGPSIQLGAMAGKGISRALDRGKTEEKFLLTCGASAGLSAAFHAPLAGVMFSLEE
                     VHKNFSVSVLISVMTASLTADYISANFLGMESVFQFD"
     gene            complement(6841..7377)
                     /locus_tag="ACOIAS_002847"
     CDS             complement(6841..7377)
                     /locus_tag="ACOIAS_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HGZ2702547.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNGVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(7450..7863)
                     /locus_tag="ACOIAS_002848"
     CDS             complement(7450..7863)
                     /locus_tag="ACOIAS_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HGZ2702548.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            8329..8946
                     /locus_tag="ACOIAS_002849"
     CDS             8329..8946
                     /locus_tag="ACOIAS_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HGZ2702549.1"
                     /translation="MDEKILELLQEMQGSISGIEGTISDMQSNINGIEGTISDMQSNI
                     NGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISSMDGTINS
                     MQDSITDMQVEIKEVNSKLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINR
                     NTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(9554..9733)
                     /locus_tag="ACOIAS_002850"
     CDS             complement(9554..9733)
                     /locus_tag="ACOIAS_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HGZ2702550.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNNYNIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(9959..10492)
                     /locus_tag="ACOIAS_002851"
     CDS             complement(9959..10492)
                     /locus_tag="ACOIAS_002851"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGZ2702551.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPDFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGINRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            11259..11447
                     /locus_tag="ACOIAS_002852"
     CDS             11259..11447
                     /locus_tag="ACOIAS_002852"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702552.1"
                     /translation="MPYKYFTEFCLLKATQFLKGTNELISDITIKVVFHQIIYFSKCF
                     KEKTGYSPKVYRNIKKEI"
     gene            complement(11913..12485)
                     /locus_tag="ACOIAS_002853"
     CDS             complement(11913..12485)
                     /locus_tag="ACOIAS_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGZ2702553.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(12547..12810)
                     /locus_tag="ACOIAS_002854"
     CDS             complement(12547..12810)
                     /locus_tag="ACOIAS_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HGZ2702554.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(12810..13031)
                     /locus_tag="ACOIAS_002855"
     CDS             complement(12810..13031)
                     /locus_tag="ACOIAS_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HGZ2702555.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         4648 a         2228 c         1491 g         4762 t
ORIGIN      
        1 aaatcaattc tttgaaattt aatttccaga attttaattt ttaagattta attttttaaa
       61 acttagtttc cagaatctta attaatagaa aggtatatta aatgggcact tattctttaa
      121 tctctacaag cataaatttt attctaatat taatcaatat tttcatagcc ttttttcttt
      181 atacgttgac ttcctatttt atagacttta ggaatcattg gattttcaaa atcatattga
      241 ttttgggcta ctctattatt agtcaaatga taatatggct ttccgacccc gtaaatattt
      301 tgtttacact attcggatat tttatactac ttataatatg tagcaaaagc aaattaattc
      361 ctataatatc agttgtcatg atattttatc ctattataat tgggcttaat tttcttttta
      421 taaacaaccc tatatatgag gaacttatac actcaaaaga gtatatttta atactttcaa
      481 tagtccagat aatttctgct ctaattaaat tattattttg gtttttaata catattttcc
      541 taaagaatag attggtcaat ataaaacaat acttatcaaa taaaatatgg atttttattg
      601 atatggtatg tatatcttca tttttatcaa tattaattgc tattattctg ccacctacta
      661 atttaatccc tatgggtagt aataagaata ttatcttagg caacttacct tatggcactt
      721 atctaatcat cttgtcagca attctggcaa atatcggtgt gatacttctt ttacagccat
      781 tgattgaaaa tgtaaagatg aagatagaaa aacaagcaaa tcatctaaaa gaaaaatact
      841 ataacttact agaaaatcaa caaattcaaa tacgcaaaat gcgacatgat atgaataatc
      901 actttcaaat gattgaatcg tatttagaaa ttgaagatat tgctggagct aaaaagtatt
      961 ttaatcagtt aaaaataggt ataggtactc ttagtggcaa acagttttgc aaaaaccaag
     1021 ctcttaactc aattttaaat cttcgtcata caagtttgga agaaaatggt gttgatgtgc
     1081 actttaatat agatatagac aatcacttgg gtatagaacc tatagattta tgtactattt
     1141 tttcaaattc tttagataac gctatagaag catctttgaa gattcctgat ttgtcaaata
     1201 gaaaagtggt attaaaggca cgttgtgaga aggagtattt tagctattta ttgacaaaca
     1261 acaaggtaaa tcctatcaat agaaaacaag aactatttat ttcagataaa aaagatagta
     1321 attctcacgg atatggtatt gagaatatta gagaaattgt taataagtac aatggaaaat
     1381 tggatatctc ttatactgag aatgaatttt ctttatttct ttatattaga ttattttagt
     1441 ctaaaattag attatcttat ataatacaaa ttagattgtt tgatactatg taaaattata
     1501 ataacttata attaaatatt tacacatata ttcattaact taaaatttaa tcaaatgtga
     1561 tattttattt accttagtaa aaatgtgtgt gtctcaaaat aaacttttta gttgatgagg
     1621 cactcttttt tacatgaaat caaatatatt tttatccttt caacaatgaa aaagggattt
     1681 atctctccat tttgaaacaa cccctttttc tattaattac tttcttaaaa aatataatac
     1741 gatatataag ataaatatct aattgtacct atttctaatt atatctatta aaatacaaca
     1801 cctttgcctt ttcagtaaac tccctaacaa aatctagttt actatctgta taagcatcac
     1861 gattatgctc atacttcttc caaaggtcca acttaagttt ttcatactcc tttgcaacat
     1921 ctgggtggtc aattaagtaa tctctaaaaa acaactcatc attatcacct acatacctta
     1981 tatgcaaatg aaaaactcgt tcttcaaacc catctactgt atacccttta ttaagtgaaa
     2041 gcctactact gctctcagac atacaaatat atccattcga ctcaatcaaa tctttaatat
     2101 ctttcatatt ataatcagga attatctcaa tcagtatgtc aacaatgggc ttggcataaa
     2161 tcgtactcac agaagtactt ccaatatgac ttattctaac aattttttgc attggcaata
     2221 ttccagttag tctatattgt tcttctttaa accaattttt ccaacattct ttgtgagtag
     2281 tcaaaaatat aggaaacaag ttccataact cctctaaagt catatcttcc aacttctttt
     2341 tcattagtta cctcctttta aatttgtact ttatattttt tctttttcaa atttacttag
     2401 ttaatattta actttatttt ttatcaaatc atttttctaa ttatgtaaat ttttctaaat
     2461 ttagttttta atgtattata acagattttt acccttctta cacttgtgta tttctttaaa
     2521 tattagaaca ctttttaatc tgcaattgta gtaaaatatt tttctttacc tgtatttact
     2581 ggcattggag cttctaaaga ataattcatt ggctttaata taattaaagt aaatttaaca
     2641 tatgtgaaag atacttatta aaattattta tattaatctc ttgttaaact ttgtattttt
     2701 taattatcta tataaacatt aatctgatat gtaggttttg aattagttct gttttcaata
     2761 ggtgtttgtt ttgaaagtgg ataaaatatt gatttagtag taattaaatc aattttaaaa
     2821 tatgtaatag aagataataa acataaaata actgctatca aaataccatt tctgtacttt
     2881 tcattaaaca tactaaacac tcttttttta atatctgact ccttatgtaa tccagccaca
     2941 aatttatttg aactatttga accttttctc attgcactaa ttattgtcaa tgcataatcc
     3001 tgcttctctt ctatcgtaca attcttcaaa actctttcat cacaataaaa ttcacaatcc
     3061 aaatctataa acttctccat tatatacaca aaaggattaa accaataaac acattttaaa
     3121 aatatcacca aatacttaat ataaaggtct ttactcttaa aatgcatcaa ctcatgccta
     3181 aatatccagt ttaactcttt taatgtataa tcacttggtg gtaaaatgac acacgaatta
     3241 aaaaatactg gactttcaac ttcatatgaa tatttaagcc gtattttttt atttatctta
     3301 agctctttta ataaattttc ataaagattt tttatcttat catcctcaac atcataagac
     3361 aaatcattta tcatattttt aagcttaata tatttcaata aagtataaac taaataaaat
     3421 atcgtaatag caatccaaac agagactaaa acagtactat atacaataga aatactatta
     3481 aaattgccta aacttatctt attaatatta ccattatatt ttgactcatt aaatataaaa
     3541 gtataattca taaatggtaa tgatagctta ataatcacaa taagccatat atagtaatta
     3601 aattttttac tgaaaatctt aaataaagtt attctaaaga ctaataaaat aactataaaa
     3661 atacaactta ctaaaattgt tctaaagata tcatttaaaa atgaactaac cacagatact
     3721 cacctttctt atacttttat atttatataa tcatctacac gaatactaaa ctgatatgta
     3781 ggtttttaat taactttgtt ttcaataggc gattgtcttg aaagtgtttt aaaagttgat
     3841 ataacaatga tttaggagta ataaggtcaa ttttaaaata tgtaataaaa tataataagc
     3901 atgaaacaac tgctatcaag ataccatttc caaactctat aagcaaaagg ggctaataca
     3961 accttaaccc cttttaatta ttttcattta taaataaatt ctattcttcc caaaatatcg
     4021 caattaaaat atattaatta aaatatccca gtaagaatat ctgaaaaatc ttctacctat
     4081 tttttcaaac cctttattcc cattcctcat acaatagtgc aacttttagc acagtataaa
     4141 tataccatta gtccatataa tctcattaac aaatatatac aattattttc ttcaaactct
     4201 tttacatcta agatatcaca ttttcatttt cttatattaa ttccttaact ctctttcaaa
     4261 ttaccaattt tttaatcaaa tcttcattaa atattctatc attattaaac attcatcatt
     4321 atccctaaaa aatatgtgac gtatgttctt tacacaaatt ttccatctta tcatacacag
     4381 caccactatc tctctcatct gtcatagtca caattacatc acttggtcta agaattgtct
     4441 tacctctagg aataatttct tctccacctc tttgaattgc taccaaaaga caattttcag
     4501 gccactgtac atcctgaatt gccttgttcg caacaggaga gccaaacata acagagaact
     4561 ggtctaatat cttttcgccc ctctctttat gtacaggttc tccatttttt tcaagtaacc
     4621 tctcaagaag actttcataa ataggtttag acttcataag tgtagccaca atatatgcca
     4681 caattgaaat tactgatagt gaaagcattt gactaacaga acctgtcatt tcaaaaatta
     4741 atatgatacc agtcaaaggg gcacgcacaa tagctgtaaa atatcctgcc atagccaaaa
     4801 gcacaaagtt atttacataa tcgggattca ttccaaaaac tttaacacct accataccaa
     4861 atattccacc tataaatgct cctaaaacaa gcaatggaaa aaatattccc cctggtgcac
     4921 ctgacccaaa actaacagcc gaaaatataa aacgtataac aaaaatcaaa acaaccattc
     4981 caaaaatata ttcatgactt gtaagttctt caactaaatt gtgtccactc cccagaacat
     5041 caggcataac aagcccaaga ataccagcca ttataaatgg aatcaacagt tttccaaacg
     5101 taccaatttt aggaatcttc ttgtataaag actgcacata tagagtaaac cagttgtaaa
     5161 atgcccccat aactccaaga ataacaccta aaatcactat taatccataa taatcttgtg
     5221 gcaacacatt tccaatctga acctcccatg actaaagtca cagggttcct gcttcataga
     5281 attttgcata ctaacatatg tcttactaat tctccacagg ctatccccgt agttcctacg
     5341 gttcttacat aagttattta ttttgaattg ttattagtct taatccttct tttaatatat
     5401 ttatacttgc atttatatct ctatcgtgag tttcattaca actaggacaa atccattctc
     5461 ttataataat gttaacaaat gtaattattt aaaacaatta tttagaaggt tttatattta
     5521 attttaaata acatatttaa gaaccgtaag tcttaacttt tatcattttt gaggttgtcg
     5581 ttcacataag ttcgctacta cctatgcagt tctcttatga acttcttaca ctttcatgca
     5641 agcacagact atatcttatc cttcggcatt atccgttaag gtctacccac ttccactacc
     5701 aatagcttgt agtgtacttc cctcaagagg aatagtcgtt gaagtttctc ctattcggag
     5761 tttacctgct gattgcccat tttaaagtac ttaggattta accttatacc atctaaccaa
     5821 ttttttctac tttcgtaact ttcacactta ggtttatttc atccttatgt tttagtttgg
     5881 ttagctttag gggtttccag caattcaagt agtattggat aggttctatc ctatctctac
     5941 atataggttt ccctatatgc tgactatttt agctactaac tcatgactaa agtcacaagt
     6001 gtgcgtagct attttttaat caaactggaa tacagactcc attcctagga aattggctga
     6061 aatatagtct gctgtcaaag atgctgtcat cacagaaatt aaaacagata ctgaaaagtt
     6121 tttatggact tcttcaagtg aaaacataac acccgccagt ggtgcatgaa aggctgctga
     6181 taatccagca cttgccccac aggttaacag gaatttttcc tctgtcttcc ctcggtcaag
     6241 agctctagaa atacctttac ctgccatagc accaagctga atggaaggac cttcccttcc
     6301 caaagcaaga ccacccatga gtgataagaa tcctccaata aattttgttg gcaacactcg
     6361 ccaccatact tgttttattt tccctgtcat ttccccctca agctgaggga taccacttcc
     6421 agaaatcatt ggctcccagt ccaccaactt tgatacaatc catgcaagta ctgcaagtac
     6481 tgcaaaccaa gctattattt ttattgtaga accttctaca aattttaaaa tttggctaag
     6541 ccaagtacca gcctgcccta aaagtatcct gtataaaagc acaatcaaac cagccacagc
     6601 cccaaccata agtccttcac caatcaaaat tacctgaaag tgctctgccc gtttgataat
     6661 atgagatgta tcctttttca tactgttcct ccttcatcca ataattctac acaatctgaa
     6721 tctccaatga ctaaagtcat gagtgtgtac agatattttt taatcaacaa aaatagattt
     6781 ttatttacta aatataagaa aataattgct caatttctca ataaatgtaa atttattctt
     6841 ttaggtattt attctccaca gtcgtgcttc ttccagcaaa atatttatta gttcttgctt
     6901 tctctgtgta tagattttac gattgtcctc aaactgtaat gccaattttt gtttatgact
     6961 atcatagagt tttgctttgt ctgtaaaagt atttagataa tctcgaaaat tgatataatt
     7021 attccatgca acaccattcc attcaacaac atgaatatgg tgagtacgag tatttttctc
     7081 aaaatctccc atagcaaaaa gtaattgttt tggtacatcc tgacctcgaa aaacgattcc
     7141 acattgctct aacctttcga ggtataattt agcttcctct aaagaagcaa cacctacaac
     7201 aatatcaata ataggctttg cgctaatact gggaatagaa gtgcttccaa tgtgttgaat
     7261 atcaatggca gtcttatccc atatactttt caataatata atgacatctt ttgcactttt
     7321 atcccatatc atctgatgtt ccaataattt aaccatacca cattttaaac ctatcactat
     7381 ataccacctc aatataatcc acctttatat atattacaat tttttctctc ttgataaaca
     7441 gtgatttagc tatttcactt tttcttgaca ctcgctacat tccctatcat gaatagatat
     7501 aattcctcca catttagaac aagtatatct ctccttctgc ttttgcataa acttctctaa
     7561 accgtactgc tgaacaaatt tactattttc cataaggctt gcttgatatc tcttgttata
     7621 acttttttca agatttttta tgagtttaca aggaaaattg aaacattcaa aacaataaaa
     7681 caaccctttt tgacagacac aatcctttat tttacactta cggcagtgtt ctggctttcc
     7741 tgtatcgcca ttcaaacaac cagcacatgg ctttttgtga taacaatgct tatagcaaac
     7801 catacaattc attccacaag gagcaaacat aatcgtatca atactctctt ttggcatttt
     7861 cataacattt tactcctttc aaaaatccaa tttgtcgaat tgttatatat tcatattgta
     7921 acataaaaaa tagctaaaaa attcatgcac tttcattgcc aataaatttt caaggaaata
     7981 tgaaagttta aaactaagaa taaaaagaga taaaaaggaa gctactaatc aaattattca
     8041 aatacaagta atcaatataa ataacttaag gtatatttaa aagtatatga tattaccagc
     8101 agttttaaat acaaatataa ccacacatgc ctcaaaaaca cttattacag tatcttagaa
     8161 acttagacaa tacataagat tcattaataa aaaagttctc aatatagata tatttaacca
     8221 tgttttgaat agcagataat catatgatta aaaattaaat atgacatagc gtttgtggaa
     8281 tataacacct aaattatgtt ataataataa aacaggagtg tgatagtgat ggatgaaaaa
     8341 atattagagt tattacagga aatgcaaggt agtatcagtg gtatcgaagg cactattagc
     8401 gatatgcaaa gcaatatcaa tggtatcgaa ggcactatta gcgatatgca aagcaatatc
     8461 aatggtatcg aaggcactat tagtagtatg caaggcaata tcaatggtat cgaaaacact
     8521 attagtggta tgcaaggcac tattggcgat atgcaaggta gtatcagtgg tatgaatggc
     8581 actattagcg atatgcaagg cagtatcagt agtatggatg gcactattaa tagtatgcaa
     8641 gacagtatta ctgatatgca agttgaaatt aaagaagtca atagtaagtt agacaatatg
     8701 gaacctgata ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgatact
     8761 attcagagtg atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat
     8821 acagtgatta aacatacctc ttcattagta aaagatacat cagatattaa aaatgattta
     8881 aataatatag agatagtcac atctaagaat tcgaatgata ttgctaaatt aaaatcagtt
     8941 aaataatata ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc
     9001 aaattttata cagaaatact gattatccga caacacactt tacttataaa taatttatta
     9061 aaaaaatact cattttatcc taaaattaac acgactaaat gagtcttgat ataataataa
     9121 aaaataaggg agattggaca ctaaaaagtt gtgtatctcc cttatatcct ataatatagt
     9181 tatataattt ttcagcctaa aataaaagtc catctttgat taaaacacat ccccttatca
     9241 cttataaaag gataaaatct tgttttcatt tttccattta tatcttcttg cttaagagta
     9301 ataatttttt cttttgatac tttaagtgtt tggttaaatt taactaatcg actaagtttt
     9361 gttcaacatc tatttttcat attttccaat acttaatcta ttttttataa ataattcttt
     9421 catttctaac tcacttacat tttttacata aaacgaaaca tataaatttc aaccttatct
     9481 tgtatggttc ttaatactta atgagtagct taagtatcaa tcattataaa tttttccctt
     9541 acatttaaca ctcttaccat ttccaaaata tacaaaatat attttgaaca tttgtacaat
     9601 gatgaaatac aatacatgaa aatacaatat tataattgtt gtctaaaaat agaagttctt
     9661 caacataacc tttcacaaaa aacatattat ctaagtattt tttgtctgct ccttgttttc
     9721 ctgtttgtag catataaatt acttcctcta aacatataac tactcacatg aaaaaagaaa
     9781 ataaacttcc aaaatacaag ctctaaaaac tacctctaaa gttgtataat gtttactagg
     9841 aataaggttg tataacatat agtttaaata atcatttttt aaaattgcaa gttattgctt
     9901 tcaaaattag atgtttgtat atcaaataaa tgattaagaa tttctaaatt taatggcttt
     9961 ataatgcact ccctttcttt gggataaaat aatgttgcac agatactttt tctaaagata
    10021 acaattttat ttttctatta attccaccag catacccagt caaactccca tttgttccta
    10081 caacacgatg acatggaata ataatagaga ttccattatg ccctactgca ccaccaattg
    10141 cttgtgctga cattttagat attccttttt gttgagcaac tttatatgca atttctccat
    10201 aagtgattgt tttaccatat ggtatttgtt gtaaaatttt ccacactgta gtctgaaaag
    10261 gagtacctac catatggagt gatggtgtaa aatcgggttc ttttcctgaa aaataaatat
    10321 ctaaccattt ctttgtctgc tcgaaaattg gcaattcttt ttcttgatgt tcttggtcaa
    10381 gattataagc ataatacttt tgattctcaa accaaagacc agttaaccca atattatctg
    10441 atgctaaaag aatatttcca attggactat ggtaatagct aatatattgc atacaattcc
    10501 tccttatatt ctgaaaactt cactcaaatc attagacact tcttttctat ctaccattat
    10561 gtaaaaacat actaatataa tttccttcta actacacttt cgttataata tctatcctca
    10621 tatcatatta gttttatatt tacctgcatc tgttcatcct tttttccctt tccctttgat
    10681 ggatataaaa ttcatacatt taagcttcct actgtattaa cttttataga agtgtatctt
    10741 ataattttat cactaaatag accaaaaaaa tatcgatata gcatcaagta atatcataat
    10801 actttcaaat gggaaaagta agaataggct gtaaaaaaga aaagtatttt tatgttagaa
    10861 atccagtaaa atcgttagta gatagaataa cagaaaacga acaatttgct atttatcatt
    10921 ttacacataa atataattga cgcaataaag tgttttctat tctacaacga ttaataaggt
    10981 tagaaaaaag taaaaatgaa ctgtatatgt atgaagcatt agtttctgtt tctttccttt
    11041 ggcttattat gataaaaaat attgttttgc caccaataaa acacaaaaat ataattaata
    11101 tccatatgca aaaaatttta cgttatattg aaaaatacta ttgttttaca ttatattgaa
    11161 gggtactatt gtgaaaactt aattttggag tactatcaag cagtggtaat atcaataaat
    11221 cagagtgctc cagatgattt gaactaagtt taaatactat accttataaa tattttactg
    11281 agttttgttt attaaaagca actcaatttc taaaaggcac aaatgagcta ataagtgaca
    11341 ttactataaa agttgttttt catcaaataa tctattttag taaatgtttt aaagaaaaaa
    11401 cgggatattc tccaaaagtg tacagaaata taaaaaaaga gatatgattt tttatttctt
    11461 ttgattatcc aaagagtaaa taaaaaatat tgtaaaaaac ttactaacaa cggcaatgct
    11521 ttgaactatg gtaatacttt gaacttttga ttggactttt tttgctaaaa taatatattt
    11581 aaatagcaaa gtaatctgtt taatcaataa aaaataagag ataaaagaaa tcaactaaaa
    11641 cttttacctc ttattttgat agtttatcag agctaatatt tacactcttt ttatttttga
    11701 tacttcatta aaaatgatgc ttattctctt ttcattttta atacttcatt agaacttatg
    11761 cttattctct tttcactttt aattcttcat tagaacttat gcttactctc cattcacttt
    11821 taattcttca ttaaaaatga tgcttactct ccattcactt ttaattcttc attaaaaatg
    11881 atgcttattc tccattcact ttcaattctt cattaaaact gatgcttgca ctctgtaaac
    11941 tcataaacat ccattaatgc ttcaccaaat ctttgatagt gaactacttc cctctcaccc
    12001 aaaaatctca atatatcttt caaatcaggg tcatcagtta aatttattaa ttgatagtac
    12061 gtagccaatg ctttttgttc agcagccata tcctcatgta aatcagttac cgaatcgcca
    12121 aagacattaa tataacttgt agtaaagttt actccattag aatctactgg ctgaagacca
    12181 tgtccaaaat tagcataatt actaccaagt ccagcagcct taagctcttc tggagtacaa
    12241 ttgcctatta attgatataa cactgaagaa attatctcaa catgagccat ttcctctgta
    12301 cctatatcag ttaaaagtgc acgagatttt ccagttggca tagtatatct ttgttgaaga
    12361 tatctagttg cagctgccaa ctcaccatta ggtcctccca actgagtcat gatatatttt
    12421 gccattctag ggtctgcttg tcttatgtta actggatgtt ccagtgtttt ttgataaatc
    12481 cacatattat cttactcctc cttaatatat atttaataat aaatctaatt ttaaagttta
    12541 ataattttat ttataaaatg atttgtccca tggccaaggt tcagatatcc atgaccaagg
    12601 atattcactt gattcatatc caaaattagt taatgctcca tatttagatt cataatcaca
    12661 cacagcttgt gtaaatttat cgcagtatct gttgtatgaa tcaagagctt tcttatccct
    12721 tggattgtta tctaaatata aatttaattc tatacaggca aattgatatt ctgaaattct
    12781 ctctaaaagt tctcttctat ttaatgacat tagtacatgt tacccccttt tttagctcta
    12841 actctatttt taggatttgc atatagccaa tcattatagt tatttgactc tattaaatct
    12901 aattcaggaa ataaagtacc tctttttaat gctgtttttg gattgtatgt gtcatcataa
    12961 atttgacgat tgatgtatgg tctagcaaat tcacaacaat agtcttgttt atcctctttt
    13021 aaatatccca ttaattatcc tccttaattt ttagaatttt aaaatagcat tatttttaaa
    13081 aattgtatac tggttctata ttataactgg tagttttaat aggtcttta
//
LOCUS       DBGJFU010000107        13084 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.107, whole genome shotgun sequence.
ACCESSION   DBGJFU010000107 DBGJFU010000000
VERSION     DBGJFU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.107"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            160..1041
                     /locus_tag="ACOIAS_002856"
     CDS             160..1041
                     /locus_tag="ACOIAS_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2702556.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1249..2088)
                     /locus_tag="ACOIAS_002857"
     CDS             complement(1249..2088)
                     /locus_tag="ACOIAS_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HGZ2702557.1"
                     /translation="MSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEYKYAVSQGD
                     YVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRL
                     ECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDV
                     SNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLS
                     RARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLE
                     KMR"
     gene            complement(2267..4201)
                     /locus_tag="ACOIAS_002858"
     CDS             complement(2267..4201)
                     /locus_tag="ACOIAS_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HGZ2702558.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4313..5326)
                     /gene="galE"
                     /locus_tag="ACOIAS_002859"
     CDS             complement(4313..5326)
                     /gene="galE"
                     /locus_tag="ACOIAS_002859"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGZ2702559.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5350..6225)
                     /gene="galU"
                     /locus_tag="ACOIAS_002860"
     CDS             complement(5350..6225)
                     /gene="galU"
                     /locus_tag="ACOIAS_002860"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGZ2702560.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            complement(6417..6737)
                     /locus_tag="ACOIAS_002861"
     CDS             complement(6417..6737)
                     /locus_tag="ACOIAS_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702561.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6727..8718)
                     /locus_tag="ACOIAS_002862"
     CDS             complement(6727..8718)
                     /locus_tag="ACOIAS_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702562.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8841..11384)
                     /locus_tag="ACOIAS_002863"
     CDS             complement(8841..11384)
                     /locus_tag="ACOIAS_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HGZ2702563.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11662..11996)
                     /gene="rnpB"
                     /locus_tag="ACOIAS_002864"
     ncRNA           complement(11662..11996)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACOIAS_002864"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12128..12805
                     /gene="srtB"
                     /locus_tag="ACOIAS_002865"
     CDS             12128..12805
                     /gene="srtB"
                     /locus_tag="ACOIAS_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HGZ2702564.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12965..13039)
                     /locus_tag="ACOIAS_002866"
     tRNA            complement(12965..13039)
                     /locus_tag="ACOIAS_002866"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13005..13007),aa:Thr,seq:tgt)
BASE COUNT         4034 a         2294 c         1333 g         5423 t
ORIGIN      
        1 taaatatatt tatcttataa ttcagacaaa aaacgataaa tttttttaaa agcttttatt
       61 cttactctat aatcatatat aataaaaatg taaaaatatc ttaaaggtta acttctacaa
      121 atatataaat aaaaatacct atgaaaggat gaacaaaaat tgaatttata tcacttacgc
      181 tattttgtta cattggcaca tttagaacac tatacaaaag cggcagaaaa tttatctata
      241 acacaaccaa gcttaagtca tgctatttct ttactagaaa atgaacttgg ggtggctcta
      301 tttgaaaaag aaggtagaaa tatagtcctt actaagtatg gaaaaatatt tttaaaagat
      361 gtggaaaaat cattagaaat tttagattca agtgtaaaat cattaaaaat aactggtact
      421 ggtgaaggac aaatagactt agcttttctt agaacacttg gtactgattt tatcccagac
      481 atagtgcaca aatttttaaa atctaatcct gctaaatcaa tagattttaa atttcatact
      541 ggtgtaacta ctgacattat tcaaggtctt aaagaaagaa aatatgatat tgcattttgt
      601 tcgaaacttg aaaaagaaaa gggaattgaa tttatacctg ttgccaaaca agatttagta
      661 ctaattgtac catatagtca tccactagct gcaaaggata ctatagattt aaaagaaacc
      721 ataccttatc ctcaaatagt atttaatcag agaagtggtc ttagatatat aattgatgat
      781 atgtttaaaa aaataaatca acagcctaat atagtctatg aagtagagga agaccaagta
      841 attgctggat tagttgcaaa aaatttcgga attgctgtag tcccaaatat gaatatgctt
      901 agctttacaa aagtaaaagt aattcagatt attcacccaa gttgggaaag aaacttttac
      961 ttagcattta taaaagatag atatttgcct cctgcaatta aaaactttaa aaactttgtc
     1021 attaaaaacg ctcaattata atttattaat aaaggggtta tttttaaata tcctctttta
     1081 ttctttttat aaaataaaaa aatctatact tttaaaagta tagattccta actgacagtc
     1141 ggcatccgag tctcagtaat tatcgttata caacagcaac tggattcagt tcctgaataa
     1201 ctaggttact tacattatat cagatttttt aaaatttgtc aattgtatct aacgcatttt
     1261 ttcaagccat ggtccaacag gctctacaat aggaacttct ttaaatcccc aatttcttat
     1321 atcattcaca ctcgttggag tatatatttt gtttctgaca gatttgaata ttttgttttt
     1381 ttctccatat tgcatacact ttggattagc ttctattaac cctcttcttg ctctgctaag
     1441 aggattacat aaaatatctg ctatttctaa accttctcta tagtcagcat cttctttttt
     1501 ggcaattata aaacccttaa ccttatctct taattcttca tctatatcta agtatattgt
     1561 tccattatta aatacatcaa aaaacgcctt ttgtactttt aagtttgaga catcatccct
     1621 tgactctata actattcgtg cagattctac agtttcatta tttacaaatg aataaaaatt
     1681 ctgaagaaca tgtataaaag ccacaacata cggttccttt gaaggtgcat aatatttctt
     1741 taatctagct ttatctactg ttactgatat tatagtacac tctaatctag atagaaataa
     1801 aggcaactca tcatagaata acttaatctg tgaatcatca aacttctcaa atgctcttga
     1861 ttttcttgat atatctgtca aatgtattcc tatgttactg gttccaaaac aatactcttt
     1921 aaatgcatcc atttctttcc tcaaagaaca tacataatcg ccttgactta ctgcatattt
     1981 atattcaaaa attactcctg tcaatgtaaa tgggtcttca tgatttatat cagctcttcc
     2041 actttcatca ataaacatta tatatttttt ctttataatc ttactcaccc tcttaacata
     2101 tgatgatagc atctatattc attttatatg agttttaaaa atattccaag atttttatat
     2161 aacttaaact taattttata ctaaaaaatt gagatgtaaa acaatcaagc ttttattgat
     2221 tgaatttcac atctcaatta taattatttt tcaatatata tttttactat tttgcaagaa
     2281 tatccattaa tttatccatt actatataag atactccgcc tcctatttga taaggagttt
     2341 caactttttt atttgcaaat actctttgtt gctctgatga taatcctgaa ttatccatta
     2401 aaactattgg tgaatgtaat tttacagcta atggtccagc tgataaagca tctactaaag
     2461 catctgattt actaactaat acagcctgta aatcagtatc tccatagaat ttttctatca
     2521 ctttggcatt agtatctact ctactatctc catatattct atttcctgaa atatcttcag
     2581 tagttatatc attcattttt gctattactg aatcgttaat tgcacttggt ccacctataa
     2641 agtatgcatt ttctaatttt ctttctgtta gccatttata agattcttct gtaattccat
     2701 ctttattgac taatattata ggttgttttt cttccccagc tttagaagct acagataaag
     2761 catctgcctc cccatttcca gaaaccatat atgcagtttt tatttcttct cgatttccaa
     2821 gttcttcagc tattcttgta gcagtttgat atctatcttg acctgctatt ctctttgttt
     2881 ctgtgttgaa agtagttttt atttcttgct ctaatttaga acttattgca ttttctccac
     2941 ctatcaagat tactgtttta ggtgctaata acttcatttc ttcaacagtt tcagttggta
     3001 aattagctgt attagtaagt agtattgttg agtcatagct acttgctaaa ggagttgctg
     3061 ttataccatc agctattgca ttaccattta ctaatattac agtatctgaa ctatcagcat
     3121 tccagtttcc aactgaactt atctttttag ctgtatcaaa tctatttgca cctataattg
     3181 tatcccatgt aggatttata gttccatctt cattaaaagt ataatctttc ccatctattt
     3241 tttgagaacc agttagcatc tttccatctt tgtctaaata gtaagactta ccatctattt
     3301 gttgccatcc tgtctgcatt attcctgttt tgtcaaagta atacttatta ccatctattt
     3361 cttgccatcc tgttactgct aaaccatcag atcctagata gtatttcttt ccattctttt
     3421 cttgccatcc tgtttgcatt actccatctg agttaaagta ataagttttt ccgtctatta
     3481 ctttccatcc agatactctt gctccatcag ttcctaaata atattttttg ttattaattg
     3541 ttttccatcc tgtttgcatt actcctgaag catcaaagta atacattttt ccatttactt
     3601 ttagccaact attcttttgg tatgttccat cagattttac ataatacttc ttaccatttt
     3661 ctgttttcca tcctgtttga tttagattta tcttatattt tgccgctatc tttacatagc
     3721 tgtcgttact ataatctata attacatctg aacctacagg aacttggtca aataaccatc
     3781 taacatcttt attgtgcatt cttatacatc caccagaaat atgctttcct atagaactct
     3841 cattattatt tccatgtatt ccatatgtat ctccataagt tccttttaat gctaatccca
     3901 tccatctatc tcctaatgga tttcttggac ttcctccagg tatattacct ttataatatg
     3961 gtctattttt tatcttgtta actacttttg ttttccctgt tggtgtttct gacccctttt
     4021 tacctgttgc aactctaaat tcttttacaa gtttattatt tacaaaatat cctagagtat
     4081 tttttcttga atttactata atcacatgtt tactggctgc gcttgattct ggtacgccac
     4141 atgcaaatac taaaactaaa gtcagtgcta gtactaaata cttttttaga ctttttttca
     4201 ctataacttc cccctctttt agaaacatta ctacatgtgt ctaattataa cataaaatta
     4261 ccaatacctt atatttacaa atttgttaca tttatacata taagtatgta ttctactctt
     4321 catacccatt aggattcata ctttgccatc tccatgaatc ttcacacatc tcttcaagtt
     4381 catacttagc tttccatcca agctcttttt ctgcctttga agaatctgca taacacatgg
     4441 ctacatctcc agcccttctt cctacaatct tatatggtat ttcttttcca cttgctttag
     4501 aaaatgcttt tactaaatca agaacactat acccctttcc agttcctaaa ttataaacaa
     4561 caagtcctgg attttcttct aatttttgta atgcttttac atgaccagca gctaaatcta
     4621 atacatgtat atagtctcta actccagttc catcatgtgt tggatagtca ttaccataaa
     4681 cactcaattc cttcaattta cctactgcta tttttgttat ataaggcatt agattgctcg
     4741 gaactccatt tggttcttca cctattctac cacttttatg agcaccgact ggattaaagt
     4801 atcttaaaag agctatatcc aatgacttat ctgcttttga tatatctact aacatctgct
     4861 ctatcataag tttagttctt ccatatggat tggttacaga aagtggaaaa tcctctaata
     4921 taggacaagt atgtgggtct ccatatacag tagctgaaga actaaataca aactttttaa
     4981 caccatattc tctcattaat tcaaataaat ttaaggtact tattagatta ttgctgtaat
     5041 attcaattgg tttttctact gattcaccaa ctgcttttaa agctgcaaag tgtataattg
     5101 attcaatatt attttcttta aatacaacat gcatttcatc tttatttctg atatcaatat
     5161 tataaaattt tactggtttt ttagataatt ccttgattct atcaaccact attgagttgc
     5221 tattacttaa gttatcaact ataacaactt cataacctga ttctaaaagt tctattgctg
     5281 tatggcttcc tatatatcct gctcctccag ctactaaaac tgccattgta attacctctt
     5341 ttctaatatt tatttttcac ttactttttt tctcatatag tccattaaat catcttttaa
     5401 attttctctc tttagagcaa agtctattgt tgcctctaaa aaaccaattt tatctcccac
     5461 atcatatctt ctaccttcaa agttataagc ataaatagcc tcttgttttc caagagtttg
     5521 aagtgcatct gtcagttgta tctctccacc tttaccaggt gtctgtttct ctaatatgct
     5581 aaatattgca ggagttatta tatatcttcc aagtatagct atatcagatg gagcttcttc
     5641 aattgcaggt ttttcaacca tatctttaac tttatatact ctatcttcga tatgttttac
     5701 atccaatata ccatatttat tagtatcttc tttagctatc ttttgaacac ctaatacagt
     5761 tgttttatac tcattataag tatctattaa ttgttttaag cacggaacat ctgaatctac
     5821 tatatcatca cctaataata cggcaaaagg ttcatcacct ataaaacttt ttgcacagta
     5881 aattgcatgt ccaagacctt ttggctcttt ttgtcttata taatgaatat ttaccatatt
     5941 tgatatgtct cttaccattt ctaacatctc tgtcttacct ttttgctcta gttctaactc
     6001 taattcaact gatttatcaa aatgatcttc tatagatttt ttatttctac ctgttattat
     6061 aagaatttct tcaattcccg attctatagc ttcttctatt atatactgta aagttggttt
     6121 atctactata ggaagcattt ctttgggctg tgattttgtt gctggtaaaa atctagttcc
     6181 tagccctgct gctggtatta ttgctttttt aactgtcatc tgcatttttt cacccttctt
     6241 cgtaaatttt ctattgtacc aaattaatat accatattta tactttttct tcaaactcta
     6301 tattatccca atatttaatg ctataagata atataaggta gatgaaattt catctacctt
     6361 tagttttata agttaatatt gtattgttta tattttatcc aaaacttata caggtactaa
     6421 atttcatcta agatgccttt tatatcacta tctatatctt catctagata agaatctaaa
     6481 tcttctaagt caacttctag ttcttgatat gtcatatcaa cactgacatc ctcatctata
     6541 tccatttctg atattaattc tcctaaaata ctatttccat cagaatctaa accactatct
     6601 atatcttcat ctatatagtt ttcatttaaa atttcatcta aatcaatctc gtaataaact
     6661 tcattatttt tttcatcccc tgagtccaat cctaagaact catagttaaa agtccaaaca
     6721 ttgtatttac ttgacatact ttattcctcc tgtgaaaaac atattttcat ctagtttatt
     6781 ggttccataa tcaacagagt tatatatttt ttcaattatg tcttttcttc tttcttttgt
     6841 actattattt aatgtcaatt gtttaaatac atatgtattt gttattctct cgaataatat
     6901 ttcttttata ctatcatcat caatatttct atagtttttc cacaataact caaataactt
     6961 aaacatttcc ctactagttt catctacata ataaggatac atattttcta ctaaatggtt
     7021 taatcttgat atggcttgtt ttaaaaatac atcaagactt gcactttcac tttcttttaa
     7081 cattctataa ttgtatttct tcttatcttc tcctgaaatt acaaagatga tattgtctaa
     7141 acttttttca acttcattta aataactgct tatattatat gataaatcag acaatagatt
     7201 tttaatctgc ttaaacactt gtacatcaag tttagtatta tacttttcaa acaaatctat
     7261 ttcttgattt tctaatattc ttggcatata tgctgacaat aaatctaaat tgtttctttg
     7321 ttcttgtatt tgcttataaa gtgaatttat aacttctatt cttatttcat catttaactc
     7381 tttttctaaa atcttatcaa attcagattt catatagtaa atatttccaa agaaattaaa
     7441 tattatcata tcattcatag ctttagacca attcttatat atcacttcaa tagaattttc
     7501 tttaagtaga ggttgctttg catatataaa tacatcttgc ataagttttt gaaccatact
     7561 tttacttaag tttaaaagta ctttttgatt attaaatttt atttccgttt ctacaaaatt
     7621 agaattttcg ttttcaagtt ctttattaat tacaatgtct tttatatcac tattttttat
     7681 agatatgatt tctttttgaa aagactttag aatagtcaag ctatcgaaat cttcttgaat
     7741 aaaaaatgga gcatgtttta atgtatatcc cactatttca ttttctatat cttttaggtc
     7801 atctatttgc ataatttctc tatttttatc atttatatgt aggttaaata catttttatt
     7861 atcatataat attatatctt ctcctaaatt taacttagaa caattatctt tcgctgactt
     7921 tacaatagtc ttcttagatt tcttatttat aaaaattaat tggtcttgat attttactaa
     7981 aagcatgttt gtgtttttat aatcaaatcc atctatattt cctgatatct tgcagaatac
     8041 tatttcacaa tctttaggaa gatttcctat tttttggtta tctttaactc tctcatttaa
     8101 atcagatggg actacactat ctatattgtt attaaatata gatacagacc ttactataat
     8161 cccctctctt tccatatgaa agtatccctt caatgacact cttttatctt ctaaaatgta
     8221 tctttctata tcatcaaaat ttatttcaaa tctatttttc ccactgataa atataaactt
     8281 ctcattatct ttattaattc cacccatata aaacttattg tcatctaaac ttaatatagt
     8341 catattatcc tttagataga atataaattc tctttgactt ttgcagatta aatcatattc
     8401 atccaaagtt attttcatag tcaaatttgc tgtaacattt ttacttgaat tttcactttt
     8461 tatgtaaaaa ggcaagttat ttccatagac aattatttga gaatcttcta aaactgcctc
     8521 taatttttct ttattgtctg aaaaactata tatatatttt acacctgtat cctcattaac
     8581 ttcatctgat ttgctgatta ttatattccc tagatttgtt atatgaagct ttttatattc
     8641 ttttgagtat ctacttatgc cactaagctc aaacccatct gaaagctcat atatgatttt
     8701 ttctcctaat ttcaacatac cttcaccacc taaacagaat tataagtatt ttataaaggt
     8761 gaataaaatg tctcaaaaca aataatattt tttttaatac tttttattct gagacatagt
     8821 ttctcctttt ttataatgct ctaagcacct tcaactattt cttcataaag tttatctaaa
     8881 tgtttaacat attcacgata tccagatttg ttctttaact cacttcttaa atcttcatat
     8941 ttttgtttaa gttcttcagc actcaaagaa ggagctgatt cttttaattc ttctaactta
     9001 taatcatagt acttatcata catagctata tatgtatcaa tagacttctt agctagttca
     9061 ttacattttt cttgtgcttc tccatattga gaataatcac ctatttttat tttatagtat
     9121 ccattttctt gaattattgt tgaagaaata cctttagctt catattcttt tgctgtttct
     9181 tttgctgttt tttcaacttt aagtgaacct gcataaacag tgtaatatac acctttacca
     9241 tctttatatg cttccatttc ttttgctttc ttttcagctt gtgctttttc ttgagcctct
     9301 ttttcagctt ttgccttttc ttctgcttct tttttctctt tttcagcttg tgcttttttc
     9361 tcttcttcag ttaatttatt gttagcttgt tgttgagttg atgactttgg aatattctct
     9421 ttatcatttt tcttattgtt gcctactgtt ataaaatatc ctacaccaac aataaccact
     9481 ataactactg ctataatagt ttcttttaat ctactcttct tagaaacaac ctcttcatca
     9541 tctaaagaat cttctttatc tgatacataa tttttcattt ctttcttgtt ttctctatat
     9601 ttgtccttag aagaattttt agatttttta ttactatttt ctttcttttt aataggtatt
     9661 actactgatt tttcattttc taatttttct tcttttattt cttttgcttt tttatcttct
     9721 aagtttttac tttctttttc aagcttcgat tcattatttg aagatacttt ttcatctaag
     9781 ttattattta ttggtttttc tatttttaca ctacgttctt cttctatatg ttgacttggt
     9841 tcttcttgtt ttatttcttt caattcattt tgctctattt cttctgattt cacttgatga
     9901 ttttcaactg gtttatctaa aattatatct ggagaaatat catttaaatt ctcttcttct
     9961 tgttcagctt cttcattgtc ttttttaata aatccaaata atttagcctt ctttttactc
    10021 tttttatttt ctggttcaat tatatcttct ttattgctgt cgtactcttt atctgtagta
    10081 tctttatctt ctacaacgtc acctatatct tgattattac tatctacttc ttctttagtc
    10141 ccttcttcag catcatcttc tattgaaata ctacttctaa agcaaatatc tttatctgca
    10201 ttttttatca tgtaaatgta ttcttcaata tcgatatctt tatttcttct agttcttttt
    10261 gatgcaattt catgattttc taattcacta tagttatctt ttattagatt ctgtgcttta
    10321 ttccatgtat cttcactaat ttctgtttta tttttaatta catagtccac aaattcctta
    10381 aaattttcag gaccaaattc tccaattata gtcagtctca ttgcaccttc tataaggtct
    10441 ctatttcttc tctttaaaaa ttctaataca actgttgcac aagcaaaatc tttaataatt
    10501 tctttgccaa aaccatatgc ttcaacagaa tgaagttctt ctaatccaca taacttctta
    10561 tattcattgt gtattgtttg agatatactt tcattattta agctatttaa cttatctata
    10621 aacttcgatt ctatatcatt tgattttgat tcatgtttta cttttgaatt tttaatttca
    10681 tttagtagca tgtcattttc tgaattatct ttacctaatt gcaaatcctc aatttcagtt
    10741 gcttttattt ctactttgtt ttgttcgtta ttctcttcca cattattagg taccacatct
    10801 ttattactag ctaatatttt gtttacacag aatataattt ctctttttaa ttctcttttc
    10861 gcatctgctt gatttttaag aaataaatct ccacattctt tcttttctaa acttattttt
    10921 ctaagagata tattaagaag gtctttatat aggtttaaaa ccttataatc cttttctaaa
    10981 cgtcttcttc tctctgatag ttccatttta tcataagcta aaaaactatt taaatcattg
    11041 gcttttagag agtctaatcc ctctttcata gccttattta catactttct acattttgct
    11101 gtagttaact ctaaatcttt ttctaattca tctatttcag gtatgtactt atctactatc
    11161 catttgccaa taagtggttc tgtatttctt gctaatgtct tataaaacaa tatattaaat
    11221 tttaaaagct ctgcatattt tgcttcatta aattctactt tttcaaattt cttttccaaa
    11281 acaccatcta gcttttcatt tattttagac tcttgtttta aaaaacttgc tgtgacattt
    11341 aaaacagtgt ttcttattat attattttca ctcaaaacct tcactcctta aatcaattat
    11401 tttattgtaa tatttacatt gtgcaaagtt gctaatttga ttatgatatc taaataatta
    11461 tttactaaat ttgctacatc ttatatcata tcatattatt tacatcattt tactttttct
    11521 ttttatatct atcttatatt ataccatttt taatatctgt actaacttat attacaatat
    11581 tcctcaaaat tttaaaagta gattagacaa atttacaaat aaaaaaacat cagtttaaac
    11641 tgatgttttt gactatcgaa ataattaagc aagtctataa gcggagttct gtattagata
    11701 atcatctttc taggcctatt gtcaccaata ggctcaagcg acctacctac cggagagtac
    11761 gagcagcact ctttattcct atcttggtct tgctccaggt ggggtttaca tagccaacta
    11821 gtcgcctagc tgctggtgag ctcttacctc gcctttccat ccttaccaca atagtggcgg
    11881 tttatttctg ttgcactatc cttaggatcg ctcccactag gcgttaccta gcaccctgct
    11941 ctatggagct ccgactttcc tcatgtaaga ttacatgcga ttatctggct tacttaatat
    12001 ttattttact tagttatgtt aacacaaatt cttttacatg tcaaagtaat attatactaa
    12061 aaatttaaat ttattcaaaa atagtgtaat atagatatat aaactacatt taactcgagg
    12121 tgatctattg aaaaaattat atagaatagt aattaacatt atactagtat tagtaatact
    12181 ttacagtgga tttaatatat actcaaaact taccaaatac aatcatgata ctaaaatatc
    12241 ctcagaactt caaaaaaaag aatataaaaa agaggattta tcaaagatta acagtgattt
    12301 taaattttgg ctttctgttg agaatacaaa tataaattat ccagttgtac aatctaaaga
    12361 taattcttac tatcttgaca aagattttta taaaaaagat tctatttctg gtacactgtt
    12421 tatggattat agaaataaat ctattgatga caaaaatatc ataatatatg gacataatat
    12481 gaaaaataaa actatgttca acaacctaaa taagtttaaa gatgctgact tctttaaaaa
    12541 aaataataaa ataaaaataa ccttgaatgg aaaagaattt ttatatgatg ttttttctgc
    12601 ttatattgta gaatctgatt atgattatct aaaaacaaat tttaacaatg aatctgatta
    12661 tcaaaattac ataaatgata ttacttctaa atcattgtat aaatctccta ttaaggtaaa
    12721 tagcaatgat aaaatagtta cactttctac atgtacctat gaatttgatg atgccagaat
    12781 ggtgattcat ggtagattga tttaaaactc acagacacag tttaaaacaa taattttaaa
    12841 ctgtgtcatc ttttatacct ataatttgta aattagacca ctaaaagtat ttaatacaaa
    12901 ataacaaata catcaaacaa taaaaaacaa taaaaaaaga ctatgctaaa aacatagtct
    12961 ttactggagc tggtgatagg aatcgaacct acaacctgct gattacaagt cagctgctct
    13021 accgttgagc cacaccagca cacctatttt attgtaaatt agcaaaacta atttttaaaa
    13081 gtta
//
LOCUS       DBGJFU010000108        13012 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.108, whole genome shotgun sequence.
ACCESSION   DBGJFU010000108 DBGJFU010000000
VERSION     DBGJFU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.108"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            200..1564
                     /locus_tag="ACOIAS_002867"
     CDS             200..1564
                     /locus_tag="ACOIAS_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2702565.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1586..2077
                     /locus_tag="ACOIAS_002868"
     CDS             1586..2077
                     /locus_tag="ACOIAS_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2702566.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2349..3020
                     /locus_tag="ACOIAS_002869"
     CDS             2349..3020
                     /locus_tag="ACOIAS_002869"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGZ2702567.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMKRLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3090..3971
                     /locus_tag="ACOIAS_002870"
     CDS             3090..3971
                     /locus_tag="ACOIAS_002870"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGZ2702568.1"
                     /translation="MRTTDMKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGL
                     YVGGSTGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGY
                     DAISAVTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENE
                     KIIGVKFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRA
                     RQIFELTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKE
                     ATDEMKSRAKEIYRKYF"
     gene            4004..5506
                     /locus_tag="ACOIAS_002871"
     CDS             4004..5506
                     /locus_tag="ACOIAS_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HGZ2702569.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5636..6088
                     /locus_tag="ACOIAS_002872"
     CDS             5636..6088
                     /locus_tag="ACOIAS_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGZ2702570.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6248..7051
                     /locus_tag="ACOIAS_002873"
     CDS             6248..7051
                     /locus_tag="ACOIAS_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGZ2702571.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7109..7996
                     /locus_tag="ACOIAS_002874"
     CDS             7109..7996
                     /locus_tag="ACOIAS_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2702572.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKDMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            8015..8863
                     /locus_tag="ACOIAS_002875"
     CDS             8015..8863
                     /locus_tag="ACOIAS_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2702573.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8926..9615
                     /locus_tag="ACOIAS_002876"
     CDS             8926..9615
                     /locus_tag="ACOIAS_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2702574.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(9945..11135)
                     /locus_tag="ACOIAS_002877"
     CDS             complement(9945..11135)
                     /locus_tag="ACOIAS_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2702575.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYAWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLDDGIDTAGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSERGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEPKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLRDSEIGYYIDTPKTKNGKRKIPLTE
                     RAYQALQRILKNRVKAQPLVVGGYSNFLFLNREGLPKVAGNYVGMVRGLIKKYNKYHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(11213..11416)
                     /locus_tag="ACOIAS_002878"
     CDS             complement(11213..11416)
                     /locus_tag="ACOIAS_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2702576.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(11798..12046)
                     /locus_tag="ACOIAS_002879"
     CDS             complement(11798..12046)
                     /locus_tag="ACOIAS_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702577.1"
                     /translation="MKHTFKKPSYYLLSQAMDGDEKAIEKVLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(12050..12469)
                     /locus_tag="ACOIAS_002880"
     CDS             complement(12050..12469)
                     /locus_tag="ACOIAS_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2702578.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQQGLKRRKEKEISFC
                     ELPEIMVENLAVWDDYETDYTIFNVCGNDIRVYDDELAEALKQLSERNRETLLMYYFL
                     EMNNEEIAKKQNISRSGVFQNRHNSLALMRKLLKEKR"
     gene            12890..>13012
                     /locus_tag="ACOIAS_002881"
     CDS             12890..>13012
                     /locus_tag="ACOIAS_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGZ2702579.1"
                     /translation="MRKTKETHTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGE"
BASE COUNT         4803 a         1605 c         2304 g         4300 t
ORIGIN      
        1 tagaataaat ttaaattaag cataataaaa tataaataat aaagttcgca ttaacaaact
       61 atttaaatat caagacctgt taaaaaacaa cagcaataaa caaataaaat agataaatat
      121 atccaattaa ggtataatta agttttatat gatatagtta tatttgataa tttacaatag
      181 taaaacttgg agggaaaaaa tgggtgaaaa gaaaataaat aatatgaatt attcacataa
      241 taattattcc aaaaagacag gtgaaccaac tagaaaaata aaaaatagcc atatcactct
      301 taaaagtaaa gaagaaaaaa tttgttctag ttgcaataaa gaaaataata ctaaggataa
      361 ttactgtaaa ttttgtggaa atgaacttta tgaaatagca tcattaagac cacttgaaac
      421 aaaactagac ttgaaatcta aaataaaaga gctttcatat catgcaaata aaagaggtgt
      481 atttttaaca acatttacaa ccatatttat attgtttatt attgcattga tatttaaagc
      541 tattataact attcaattta atgatattag ttatttagta aatccagcac atataatcct
      601 agctctcaac ttgggtcaaa ttagtgtatc tatgtctacc atgatgggtt ctgggtttat
      661 aaatgccaat ataggtttgt tgatattact tataatccct attttggtat tattaatatc
      721 aaacctaatt tttatgagaa aaagatgtag agattcaaaa actgtattag caaattctct
      781 aggagttgga attttttatg gattagtttt agctatttta tctattttta ctaatgtaaa
      841 aactagttca catagtatgt tagaatatgg atatgcatta gaatatagtt atgaattttt
      901 tagtgttctt ttaaatggat tcgtattagg gtttatatgt acatacataa ctacttataa
      961 aaaaagttat gaaaaagaaa atatgtattt atctcttttt tcaaattcta taagaacatt
     1021 catattaggt tatgttttag tattagtaat actattggta ctaactatat ctgatagtag
     1081 ttacttaaat gaactagata tgagtagtta ttcaaatgga ttaaatttat ttactatact
     1141 cccacaaata gctagttata tgtgggcttt tgcaaatgga atttcagtaa ccataataaa
     1201 ctcaacagta tcaatgttta ctttaagttc ttcatcttta tttggagata ctaaattaat
     1261 gttttatgca atgggagcat tgtctatgtt aatattatta ttaaatggat acaagctaag
     1321 atttaagtat aatacagata gtataagacc tataatagta tttagtatat attatgcatt
     1381 tttgatggga atattggcct tatttagtac atttatacta gatagtaata ttaatttctt
     1441 taatacaact aattatggaa caactttaat aatgcaattt aaagtcttac aagctattgt
     1501 aatttcattt gtatatagtt ttgtgatatc tttaatagga tataagctaa attcagctga
     1561 ttaaatatga ttggagggaa tttctgtgga aggaaatact aataataaag tagatgataa
     1621 attttctaag gttaagaaaa agtttggtag tggaaaattt aaagttgaat tatataaaga
     1681 aaaatcaaaa ttaaatagag agattgatga tattcaagaa aagaaagcaa agatattctt
     1741 ggaaatggga atactgacat atcaaaaaat tagagaagat tctattaatg ataatagttt
     1801 tgataaattc tgtgatgagt tactagaact tgataagtta atttatgaaa aaaatatgga
     1861 aattaatgaa atggaaatgg cagaaagtaa tgtaacttgt gagtgtggtt atgttggaaa
     1921 tgtaaatgat aaatattgtg ctgaatgtgg taaaaaattt gaattggaaa atgaatgtga
     1981 tgattttata atttgtggtt attgtgaaag tgagatagat acagaggctg aattttgtcc
     2041 atgctgtgga agaaaaatta taatggattt agaataatat tgggaaacct atgtatagta
     2101 tacatgggtt tttttattga aattatttaa atggacttat aacagtagtt atataaaatg
     2161 aaaataaaca ccaaaaaaat gaaataaaat ttcttttaaa tgtaaaaaac aagctaatat
     2221 ataaaataaa tcagtaaaaa tatagatgcg agaaaaaata tgaaaaaata ttgcaaagaa
     2281 aaagttttca tgttattatt aatacataaa tcaagaaaat aaatttcaga aacgaggtaa
     2341 tttaaaaaat gctagataag gtaaagggaa gattaatagt atcatgccaa gctttagaaa
     2401 atgaacctct tcatagtcct tttataatgg ggagaatggc taaggctgct atggaaggtg
     2461 gagctgttgg aattagagct caaggagttg aagatattat tgaaataaaa aaagttactg
     2521 gacttcctgt tataggtatt ataaagagaa actatgaaga ttcagatata tatataacgc
     2581 caactaaaaa agaagttgat gaacttttaa caacagggtg tgaaatgata gcattagatg
     2641 ctaccaatag agttagacca aataatgaag acttaaaaga acttataaag tatataaaag
     2701 aaaacggtgt tctagtaatg gcagatattt caaattatga tgaggctatc aaagcacaag
     2761 agtacggtgt agattgcgtt tcaactacac tatctggata tactccatat actaaaacat
     2821 tagaaggtcc agattttgta ttaatgaaaa gattagttaa agatttagaa atacctgtaa
     2881 tagctgaagg aaaggtaaat actcctcaag atttaaaaaa ggtctttgag ttgggtgttc
     2941 attcatcagt agtaggttca gcaataacaa gaccacaatt aataacagaa aaatttgtta
     3001 aagcaattga aattaattaa aaattaattt ataaattaaa aaattattat atttaactat
     3061 atttgaaaat taaaattgga ggtaaagata tgagaacaac agatatgaag ggaatttatt
     3121 cagcattatt agtttcattt gataaggaag gaaatataaa tgaaaaaggg ttaagacaaa
     3181 taattagaca caatatagat gtttgtaaag tagatggtct ttatgtagga ggaagtacag
     3241 gggaaaactt catgctttca actgatgaaa agaaaagaat atttgaaata gctaaagatg
     3301 aagtgaaaga agaaataaaa ttaatagctc aagttggttc agttaattta aaagaagctg
     3361 tagaacttgc taaatttaca actgatttag gatatgatgc aataagtgct gttacacctt
     3421 tttactataa atttgatttt gaagaaataa aacactatta taatacaata ataaattctg
     3481 tagataatag attaataata tactctatac catttttaac tggtgttgat atgagtttag
     3541 accagtttgg agaattattt gaaaatgaaa aaataatagg tgttaagttt acagcagcag
     3601 atttctactt attagagaga atgagaaaaa cattcccaaa taaattaata tttgcaggat
     3661 ttgatgaaat gatgttacca gctacagttt taggtgtaga tggggcaatt ggttctacat
     3721 tcaatgtaaa cggtgtcaga gctagacaaa tattcgaatt aactaagaat gaaaaaatat
     3781 cagaagcact tgaagtacaa catgtgacta atgatttaat aacagatata ttaggaaatg
     3841 gcttatatca aacaattaaa ttactacttg aagaacaagg tgttgaggct ggatactgta
     3901 gacaacctat gaaagaagca actgatgaaa tgaaatcaag agcaaaagaa atatacagaa
     3961 agtattttta ataaaatact tttagtatgg gaggattagt caaatggtag gatttacttg
     4021 gatagatttt gcaatactag tggtttactt attggcagtt ttatttgcag gattgttatt
     4081 ttcaaaaaag gaaatgaagg gaaaagagtt cttcaaaggt gatggaacta ttccgtggtg
     4141 ggttacatca gtatcaatat ttgccacatt attaagtcca atatcatttt tatcattagc
     4201 aggaaattct tatggtggta cttggatttt gtggtttgca caattaggta tgtttatagc
     4261 aatacctttg acaataaaat ttttcttacc tctatatagt aggttagaaa tagatacagc
     4321 atatgaatac ttagaaatga gatacgaaag taaaggtctt agagtacttg gagctttgat
     4381 gtttataata tatcaaatag gacgtatgtc tattataatg tatcttccat caatagttct
     4441 ggctggtctt acaggaatac ctgtaaatgt actgataata gttatgggtg taatagcaat
     4501 aatttactca tatacaggtg gattaaaggc tgtcttgtgg accgatttta tacaaggaat
     4561 ggtattaata gtaggtgtta cagggacatt gttctattta atagcaagta taaatggtgg
     4621 ttttggaaca gtaatggata ctcttacaac tggacacaag ttcttagcaa gtaatgaagt
     4681 tatgtttgat aaaaatatac tatctacaag tgcatttata atatttgttg gagctggtct
     4741 taatactttt tcatcatatg tatcgagtca agatatagtt caaaggttta ctacaacaac
     4801 tgatataaag caacttaata aaatgacata tggaaatggt gttttatcaa tgggacttgc
     4861 aacagtattt tatctaattg gaacttgttt atttatatac tatactcaaa acccagattt
     4921 agctcaaaca gttcaacaag accaagtatt tgcatcatat atagcttatg aattaccagt
     4981 gggtataaca ggaatactac ttgcagcgat atatgcagct tctcaatcaa ctttatcgac
     5041 agggcttaat tcagttgcta caagttggac tttagatata caaactatca taacaaaaga
     5101 tataagtatg gaaaagcaaa ctaagatagc acaatatata tcattaggtg taggtatact
     5161 ttctatagct gttgcaatag tattagcaaa tggagaaata aagtcagcat atgaatggtt
     5221 caatagtttt atggggctag tactaggtgt tttagcagga atatttgtac ttggtgcatt
     5281 ttgtaaaaaa gcaactaaaa tgggagcata tgttggattc atagtatcag caattttagt
     5341 tgtttattta aaataccgta tgccagaagt aacttcttgg gcatattctc taataacaat
     5401 cacgacttct gtagtggtag gtacaatagt tagtgcaata gaagctaaag ttaaagcaaa
     5461 agtatcttta ccaaaagctg agactactgt ttattatgaa aaataatatt taaatgataa
     5521 attgtcttat agagtgatta gataaataat ttagttactc tataagattg tataatatac
     5581 tttgtataca aaagtataaa aatagacttt ataattatat agggagatag tgcttatgat
     5641 atttggaaat ataaatcata gtaagacata ttcaacattg catgaagatt tacttagatg
     5701 ttttgaatat gctaaagata attcattaat agattatgaa aaaggtactt atacaataga
     5761 tggtgatgat atttttgtga atattgtaga gtataaaact tgtgaaaaag aagatagatt
     5821 ctgggaagct cataagaagt atatagatgt tcatttgatg cttgatggat gtgaacgaat
     5881 agatataaat tttatagaaa atttagaaca aaaaatatat gaagaaaagg gcgatttctt
     5941 gcctcttgat ggtaaaaata atggatacat ggaacttaga aaaggtgatt ttttagtttg
     6001 ttatccggaa gatgctcata tgacaggaat aaaagtttta gaaaaagaaa atatcaaaaa
     6061 agctattttt aaggttttgg ttagataatg ataatttata taaccactag ggctgaaaat
     6121 agtgatttaa ctttcagcga ttagtgagac ttcgtaaatt ttagttagat tactattttc
     6181 agccttagtt ttttatgaga aaattaaaaa taattaaaag cttaaaattt tgtaaaggag
     6241 ttaacttatg gaaaatatag atgtattctt attaatagga caaagcaatg ctagaggatt
     6301 gggaaatcca aaagagagca tcattccaaa tgaaaattgt tttgaatatt tgagtactga
     6361 tgaaattata aatatgagat gtgagctaga gacttcagaa ggtgatggta ctatagcacc
     6421 tgcattttca aatgaatgga ataagcttac tggaaataaa gtttgtttta ttcacaatgc
     6481 aaaagatggt tcaagaataa aaaactggaa tcatgataat aactggtttc taaatgacac
     6541 tatagaaaaa tttaatacag gctgtactac cttaagtaaa cattataata tagaaaataa
     6601 atacgttatt tggattcaag gtgaaagtga tgcaaaatat ggtagtgatg tattgtacta
     6661 taaagaaagt ctaaaaaaaa ttgcgtatag gcttaaagaa gaatgtatga ttgataaaat
     6721 gtttgtaagt ttaactggtt attggcttgg agaagatgaa tattttatta gaacaagacg
     6781 aattgcagca gcacaggagt cggcttgtag tgaatgtgat atattatgtg taggaagcaa
     6841 aattgctatg cagtttcatg acagaggatt aactattgat gatgttcatt acactcaaga
     6901 aggtcttaat atgttaggag aggacttatg taaaaatatt tataagtatc atataaccaa
     6961 agaaaaaagt gtattaagtg atactataga cttatcagaa gcaaggaaat atatctgtga
     7021 attggaaaag ataagtaaaa gatttgtgta atataaaatt ttaaatagag ttgttaattt
     7081 ataaaaataa tttgtatgga gatatgttat gaaaaattac gtttgtatag atgttggagg
     7141 tacttcaata aaatatggtt ttattagaga agatggattt ataattgata aatcaagtct
     7201 tgatactgaa gcaaaggaaa aaggtggaga aggaatatta gccaaaataa aagatatagt
     7261 aaaaaaatat atagaggaaa atgaaataag tggtatatgt atatcaactg caggtatggt
     7321 tgacccagtt gaaggtaaaa tactatttgc attagaagaa ttaattccaa actataagga
     7381 catgcaatta aaaaaagaag tagaaaaaga gtttaatata aaatgtgaag ttgaaaatga
     7441 tgttaattgt gctggacttg gtgaaatgtg gcttggagca ggtagaggtg caacatcttc
     7501 tatatgttta acaataggta ctggaattgg tggatgtatt ataataaata ataaactgat
     7561 aaatgggttt agtaatagtg ctggtgaagt aggatatatg aatgtaaatg gaagttcttt
     7621 tcaagaattg gcatctacta gcagtctaat taaaaaagta gcaaaaataa aaaatttgaa
     7681 tgaaaatgac ttaaatggaa aaataatttt tgatttagca aagaataatg atgaagattg
     7741 tttaaaagaa ctcgataata tgataaaatc attagcagtt ggaatcgcaa atatatgtta
     7801 tgtaataaat ccagaagtgg taattttagg tggtgggatt atggcacaag aaaagttcct
     7861 aaaaccaaaa atagacaaag cattaaagga ggttcttata gagagagttt acaaaaacac
     7921 taatatagaa tttgcaaaga gacaaaatga tgcaggaatg cttggagcat tatataattt
     7981 tttaaacaaa atataaagaa tagaagggtt tttaatgagt attttagaac aattggaatc
     8041 accaactttt aaagtcacaa aatcagataa attattaata gcttatataa aagaaaatat
     8101 agatgatgta ttttataaac caatatcaca aatagctaaa gagagcaata ttggtgaagc
     8161 aactataact aggtttgtaa aaaaaatgaa ttttaatgga ttacaggatt ttaaagttac
     8221 tttagcacaa gaaatatcaa ctatcaataa aaaaaatatt ataaataaaa atatacaaaa
     8281 tgatgagcca gctttggata cagcaaaaaa acttttaagt tcaaatgtaa ccactttaga
     8341 gaacacagta gagataatca atagtaagga tgtacatgac tgcgcaaggc ttataataaa
     8401 tgcaaagaaa gtttacttca taggtattgg atattctggt ataatagcac aggattcaaa
     8461 ctataaattt atgagaatag gtcttaattg tgtaagtttt gacagtagtc atacaatgat
     8521 aatgatgtct tccataatgg aagaaggaga tttaataatt gcaatttcac attctggtga
     8581 aacagaggag attataaaaa ctgtaaagct tgcaagagcc aataatgcta aaattatatc
     8641 aattactgaa aataaaaatt ctgaattaaa ggatatttca gatgtacact tatcttatgt
     8701 atcgggagaa acagtactgg aaaccggttc aatatcttct aaattagcac aatttttcat
     8761 tattgattta gtatatacac aggtagtaaa agaactttct aatgaagcta ttgaaaggaa
     8821 aattaaaact acaaatgcta taaaattgtt taaaaatgaa taaaataaga aatttgtaat
     8881 gttttataga gctctcttaa aaatttagga atggagagat attacatgtc taaattaaaa
     8941 gattataata ttgataaagt aataattttt aataatttaa gtactaaaac gaagaatcaa
     9001 cttaaagaaa aagttaaact aaaaagatta caaaaaaaag aaattttatt ttatgaaaaa
     9061 gatagcttag acagtgttta tatactttta gaaggaaagg tatctatttt taagataagt
     9121 gaaaatggag agagaaaagt aattttcata ttaaatagtg gggaagtcat aaatgatatt
     9181 acacttgacg cttctaaaaa ctctactgtt ggctgtgaag catttgaaaa ttcaattgta
     9241 gcttattata gtattgaaga atttttagaa attatgcaaa atgattttca acttacaaag
     9301 gatattattt cttatatgga aagaagaata agacgtttat atagacagtt gaaaaattct
     9361 atatctataa aagtagataa aaaacttgca gcaaaattat ataggttaag tagagaattt
     9421 ggagtttgct gtggagaatg gactttacta aatgtaaata ttacagttac atatttagct
     9481 gatatgcttg gctgtaaaag ggagactgtt tccagaatga taaaggtatt gcaaaaagaa
     9541 gaacttatta aaattgacaa taaaggtttt tatgttaaag aaactgaatt atcaaggtat
     9601 tttaaaaata attaattatg attaagtaag agtattatta gaattttaat taaataaaaa
     9661 tattattaag agaatctatt tatagattct tttttattgt ctaatttatt aatttttatt
     9721 ttaccctatt gaattgtata aaaaaacaaa taagattctt ttttattcaa aaaacttact
     9781 aaatttttga ttatcttttc ataagttggt aaatgccgat atatcccgta tttgagggct
     9841 ttatcggtat tttctttttg gaagatacct tgcataagct ggcatttacc agcatgaaaa
     9901 tgcaaccaaa agtagtaaat gagtagtaaa tatacgttcc gatattaaca agccagtctt
     9961 tccagttctg cttttgcaga ttggaatgta ccgtgtgcgt agtagccaag cgtcatggtt
    10021 atgttagcgt gtcccatgag atattgcagg gtgttcgggt tcattccctt gtttgccata
    10081 ttcgtacaat aggtatgtcg gaatgaatgt ggtgtaatgt ttggtaactt gtctttgtgg
    10141 tatttgttat acttcttaat cagtcctcgt accataccca catagtttcc agcgacttta
    10201 ggcaatccct cacggtttaa gaataggaaa ttgctgtaac caccaacaac cagcggttga
    10261 gcttttactc tgttctttag tattctttgg agtgcttgat aagccctttc cgtcaatgga
    10321 atttttcgtt ttccgttctt tgtttttgga gtgtcaatat agtagccgat ttcgctatct
    10381 cttaatagct ggtggtctat attgagtatt ctgttctgca tatcaatatg cgttgtcagt
    10441 ccgcaaaatt cagaaatacg aagtcccgtt tccagaagaa gcacaacctc atcataatac
    10501 ttgctgtaaa tcttatccgt ttccataaag gcaagcaggc gttcttcctg ctctggcgta
    10561 agaataactt tcggctcggt atcatcttcc agaacatcac ttagcttaaa ttcaaacggg
    10621 ttctttctga tacagtcgtc ttgtatcgcc atataaaatg acgctttcaa ggaacgctta
    10681 tagttgtcaa tcgttttgta ggcatatcct ctttcactca ttctgatagc ccactcttta
    10741 gcgtccgact gtttgacggt atcaatcgct ctcataccca atgggtcatt tttcagagcg
    10801 ttcataagat actgtcgtcc tttttccgta gctcgcttga tgttctttct ttggctgttc
    10861 ttcttgtcgt aaagctggca gactgtcatt ttaccgcctg ccgtgtcaat accgtcatca
    10921 aggtcttttt ttatctgtgc ttctttttcc ctcaacgata tatcatcacg ttttccagca
    10981 ggcgttttgt ctgtcggtac aagtttccaa gcataaataa actgtggctt tccgtatata
    11041 tcggtatatt tataaacgta tcttccgtct tttcgctggc tctctccatt acgcaaattg
    11101 cgatttttac tgtcttttcg tttcacatta gacatagtgt aaagctcctt tccgtcatgg
    11161 aatgagccgt gatacgctat acttattata ccatatccac ggctctatga cattagattt
    11221 cgtccaatgt atctatgatt ttttcaaatt gttttcgctt tatctgaata cggttgccgt
    11281 tcacgataac ccagccagcg tcaatatttt cttcggctaa tttacgcaac ttcttttctc
    11341 caatgcggaa gtatttggac gcttcttcaa tcgtcagtgt atatttttcc cagataggca
    11401 catcagtatt attcataatt tatgcacccc ctttactgtg tgtcagtcta taagttatat
    11461 aaacaggcag acggctttgg aaagctccat tagtatttca actattccca ttcttgtggg
    11521 cagaaccgca ccatgcggaa gtatcattat gctgtaaggg taggtcatgg caaaacgact
    11581 tttccaacgg tcgctctcgg atcactgcgt gggtcgctcg ctttcttttg gaaaggtcat
    11641 ggcgtacagt tcccgtggct ctctatcctt acaaacgtat ctgtctgctt tattcagttt
    11701 tcaaagagct gtggcgaaag cggtatgctt tcatgtaaaa gcaggagcag agcaggaaag
    11761 aggggattga acaaatcatg ctctgcgtta ctgctggtta ttcttcttca atttctaagg
    11821 caatatcaaa ccctaaaatc attttgataa gtgcttctcg gataagtcct tttaactcca
    11881 tatctacaac gatataaaca ttaccgtatt catcatagag tggacgcaaa cagcacttcg
    11941 atatgtaagg gtcataaaat gcaagtacct tttcaatcgc tttttcgtcc ccgtccatag
    12001 cttgtgaaag caagtagtaa gacggcttct taaatgtgtg tttcatttgc tatcgtttct
    12061 cctttaatag ttttctcata agtgccaatg aattgtggcg gttctgaaaa actccgcttc
    12121 tggaaatgtt ttgctttttg gcgatttctt cattgttcat ttccaaaaag taatacatca
    12181 acaaagtttc tcggttacgt tcggataact gtttcaaggc ttctgccaat tcatcatcat
    12241 agacacgaat atcattaccg catacattga aaatcgtata gtctgtttcg tagtcgtccc
    12301 atacagccaa gttttcaacc atgatttctg gaagttcaca aaaggaaatt tctttttctt
    12361 ttcttcgctt caatccctgc tgatagtcct tgacaacatg acggacaact ttcttcaaac
    12421 agctttcaaa acggcattga acagttttct ggaagtcaga cggtttcatc aatctcaccc
    12481 cctttccgtt atgacgtgaa aagtgtttat ccctctttcc gcaccatata gggaatggca
    12541 agtgtgattt gttaagttga aaccagaaaa aatcgaaaaa actttttagt aaaacaaaaa
    12601 cagcccgtgt gaatgtgaat acacaaacgg actgctttat aaatgctatc tgtatgaatt
    12661 tttaactctt gtagagggta gaatgattgt aatactgaac taaaatattt tttcagttgg
    12721 gaacagcaaa gtattattcc atatacaatc atggcgtagc cccctttacc agagctatac
    12781 cttgtgccac caatgaaaaa ggacggctct ggctatatcc aaaaccgccc cgttatgttt
    12841 tcaagacgca caaaagtata tcatctcgga acggttatgg tgatttaaaa tgagaaaaac
    12901 aaaagaaaca catacttttg atttcagacc gctagggctg gcaatcagag aagcccgtga
    12961 aaaagctggg ctttctcgta atgatttagg cgataaggta ttctatgggg aa
//
LOCUS       DBGJFU010000109        12976 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.109, whole genome shotgun sequence.
ACCESSION   DBGJFU010000109 DBGJFU010000000
VERSION     DBGJFU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.109"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            249..632
                     /locus_tag="ACOIAS_002882"
     CDS             249..632
                     /locus_tag="ACOIAS_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HGZ2702580.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            1140..1523
                     /locus_tag="ACOIAS_002883"
     CDS             1140..1523
                     /locus_tag="ACOIAS_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGZ2702581.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1525..1875
                     /locus_tag="ACOIAS_002884"
     CDS             1525..1875
                     /locus_tag="ACOIAS_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702582.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(1976..2434)
                     /locus_tag="ACOIAS_002885"
     CDS             complement(1976..2434)
                     /locus_tag="ACOIAS_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702583.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2479..3267)
                     /locus_tag="ACOIAS_002886"
     CDS             complement(2479..3267)
                     /locus_tag="ACOIAS_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGZ2702584.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3406..3843)
                     /locus_tag="ACOIAS_002887"
     CDS             complement(3406..3843)
                     /locus_tag="ACOIAS_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGZ2702585.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            complement(4077..4211)
                     /locus_tag="ACOIAS_002888"
     CDS             complement(4077..4211)
                     /locus_tag="ACOIAS_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702586.1"
                     /translation="MGILEGAAMIREIAIKIAKEKGITEQKAWPEAVKEFKEKYELVL
                     "
     gene            4665..5174
                     /locus_tag="ACOIAS_002889"
     CDS             4665..5174
                     /locus_tag="ACOIAS_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702587.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            5201..5347
                     /locus_tag="ACOIAS_002890"
                     /pseudo
     CDS             5201..5347
                     /locus_tag="ACOIAS_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            5600..6019
                     /locus_tag="ACOIAS_002891"
     CDS             5600..6019
                     /locus_tag="ACOIAS_002891"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2702588.1"
                     /translation="MVLAGILIVVQGFLTGPLAKKIGEKRLILIGMFCKALVAFAMVL
                     TTGFVSVLVVFGLFAVSSAITVPTLNATLSKTDNQHKGTLMGFASTAGNLSKVIAPLL
                     TGYLYESNIELPYITTGGIALIGVVICYIWIKNQKNK"
     gene            complement(6206..6399)
                     /locus_tag="ACOIAS_002892"
                     /pseudo
     CDS             complement(6206..6399)
                     /locus_tag="ACOIAS_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            6501..7007
                     /locus_tag="ACOIAS_002893"
     CDS             6501..7007
                     /locus_tag="ACOIAS_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGZ2702589.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSALDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            7012..7260
                     /locus_tag="ACOIAS_002894"
     CDS             7012..7260
                     /locus_tag="ACOIAS_002894"
                     /inference="COORDINATES: protein motif:HMM:NF027483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HGZ2702590.1"
                     /translation="MLITCCFGKDFNLSKFAFEIEHEITPMEQMKAINFSLEKDDCTS
                     KVLIYNPDKWKYVEYYFYKDRPIKKTIGKIYSILHLSQ"
     gene            7404..8129
                     /locus_tag="ACOIAS_002895"
                     /pseudo
     CDS             7404..8129
                     /locus_tag="ACOIAS_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            8232..9047
                     /locus_tag="ACOIAS_002896"
     CDS             8232..9047
                     /locus_tag="ACOIAS_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2702591.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            9128..9604
                     /locus_tag="ACOIAS_002897"
     CDS             9128..9604
                     /locus_tag="ACOIAS_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HGZ2702592.1"
                     /translation="MTTYKVNSIGTICNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTNFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSAVEIIDIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWESEFNF"
     gene            9618..10181
                     /locus_tag="ACOIAS_002898"
     CDS             9618..10181
                     /locus_tag="ACOIAS_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGZ2702593.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPELLFDKSKKSTKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKHYQGNGLMSEVLKNVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITRWCYG"
     gene            10460..11347
                     /locus_tag="ACOIAS_002899"
     CDS             10460..11347
                     /locus_tag="ACOIAS_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2702594.1"
                     /translation="MSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKRLVKKSVQE
                     YIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDEYRKNVPML
                     NTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNISEQIPAGE
                     STGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAGGMVSSTDV
                     CPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNLVTKPFMAE
                     KYYKGNAEINYMEIWIIPV"
     gene            11425..11859
                     /locus_tag="ACOIAS_002900"
     CDS             11425..11859
                     /locus_tag="ACOIAS_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HGZ2702595.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            12297..12976
                     /locus_tag="ACOIAS_002901"
                     /pseudo
     CDS             12297..12976
                     /locus_tag="ACOIAS_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
BASE COUNT         4695 a         1837 c         2188 g         4256 t
ORIGIN      
        1 ttataaagaa gcaagaatac tcaataaaac ttaaagaaat taaacgattt ctaaaaatac
       61 cttgaaaata gaaccttatt tgccatttat aaattatata aaaagggatt atataaagtc
      121 atatatgaaa aaactatatt atactatctt ttctttttgg aataaactat actttaaata
      181 tagatattta atttaaataa attttacagt attaatagta aattatctta gaaaggaatg
      241 tgctttatat gtctacagac aaagttgttg gaatcataga tgaagaaacg gcagaacttg
      301 caggaattga atatactggt aaaatttatg cttcttctgg tgttataaaa catataaaga
      361 aaaaacatag atgtcaactg agtaaggata tttttaatga tataatagac actataaaaa
      421 tggtattaaa gtcacctgaa tatataggaa gtcatcctaa aaaacctgga aaaagtgtgg
      481 agtttataaa aaaaattgat gattatattt tagtagctac agaattagat acaaaaaaag
      541 gatatttata tgtatcttca atgtatgcta taaaagaagc aaaattagaa agtagagtaa
      601 acagtggaag agtaaaacaa tatgatggtt aacataatat tattaatttt ccctaaaata
      661 ttgaaaaata ttattatata agttataata ttaatataca cgtagatatt tattagttag
      721 atttgaggtt ggaaaaggca cccaatacgc tactatagag tagttaatag agatgcagga
      781 tacgccgccc tgccaaatct aattaaatta aaaagagagg attaattcct ctcttattgt
      841 ttttttgaga tactaattta atctataata gaaagattga aggtcgttta tacaaaaaaa
      901 ttttcagtta tatacattaa actaattaaa taagatattt ataatctata tgtgatacat
      961 attatatatt aataaaagaa aaatatatag aaaatcctta taaaaattta aattgtaatg
     1021 aataggaagt aattaaaaag aatttttatt gatttttaaa aatttagtta gaaactatat
     1081 cattatggta aaaaatgtat tataatgttt agaaatatta taataaggag cgattaaaca
     1141 tgaatctatc gaagaaaata gaagataaaa gaaaagctaa acagagagct gaaaaagtta
     1201 agaaagctaa aattgctaca gctggtgtgg tattaggagc tgttactggt gcagtgagtg
     1261 gagtactttt ggctcctaaa tctggaaaag aaacaagaga ggatataaaa gatgcttcac
     1321 aacaaattgc tgaaaagata aatatgaaaa ctgtagatgt aaaaggcaaa gtatctgaga
     1381 agctagagga taaaaaagga aactttatag aatcaaagaa aaaaataaaa aaatatatag
     1441 ataacaaaaa gagtgttgaa aattctagtg aggaagataa aataatagat atagttgaac
     1501 cagttagtgt tactgaggaa taatatggac aatacaataa cgattaacat aagtataata
     1561 tatgtattat tagctatact tgcttgtatt gctttagttt atttaattat tgtacttgtt
     1621 aaactacata agattttaaa taatgttgga actatgttaa atgaaaatac tcaaaatata
     1681 aatacaactt taaataatct tcctaatgtt gtagaaactt ttggagaagt aggagaaaat
     1741 atgaaagatg taacagaagt agttactgaa gttacagcag attttcttgt tactaaagac
     1801 agtgtcaaat caaatatgga tgttatagtt gatatattga ctataattaa aaacattttt
     1861 gtagataaaa aataataagt taatataaaa taagcctata agatataatt taaacgacct
     1921 taaaatagca tttataaggt cgttttttat tagcttgatt tgacactatg tgtttttaat
     1981 aattggtaat attcctcttt atcatttgaa catatatatc tatgcttgtt gtttcaatta
     2041 catccataag aacatcttta cattcaagct caaaatcttc cattccttct aaatattcat
     2101 catcttcatc aaaaaagata taagaattat atagccattc ttcaaatttt tcttcaagta
     2161 tatcccaata cgaactccaa aataatccac aacctgatat ataagatgct ctactttgat
     2221 ttatataaac ttcatttaga agcttttgta atctactgct ataaagaaaa tctgctcttc
     2281 cactgcttga tattaattca tttaaatctt ctattgacat taattcaact ttaccacttt
     2341 tatcataagg agtataattt tcatcaaccc atttatgtgg tttgttttct ctgcatactg
     2401 ttgctatcaa ctctacaact tttgttctta tcattttttt atcctaactt atttattttt
     2461 tataaaataa tcatgatttt ataatttatc tatttcttgt aatgctaatt taacagtttc
     2521 atatctatca gcaccattta ttactacatc accttttaca agtttattag cttctccacc
     2581 tacaacataa acctttttac ctaaaccttg cttataacta gacgcttcaa ttattaaaga
     2641 atctttcaat tgccaatgta aaatctgagc agccacttta tcagcatcac ctgtataaac
     2701 gattacattt tcatacactt tattttcccc tcccccttga gaatttgata tagatttgtt
     2761 tagtatacct tctgctatca atttagcaac tttatcttta ttattaacat aataatctgt
     2821 atctgcttta ctatctacaa aacatacttc tattaatatt gctggtgcaa tagtatgatt
     2881 aagccaataa agacctctaa catcagactt tgcaccccta tctttgaata ctgtagctaa
     2941 cctagtattc actctttctg cataggtttt gccattatta gtcttatata ttgtttctgt
     3001 ccctactgga gttgaagttg tagcatttga attaaaatga atctgaacag ccaaatcagt
     3061 ttcctgttta tttgctatag cacattggtc tgctaaatga ttacttgatt catcaactct
     3121 tcctgtataa acagtagctc cacctatttt aagccattta acaattaagt cattcagtat
     3181 tcttgtttct ttgctttcgt ttatataccc aacagctcct gtaccttttc caattaaagt
     3241 gtgccctggt attattacta ctttcaaact aacatctcct ttacaattaa atttaacttc
     3301 tatttaatat atgcataacc tagaattttg tttcttttta tatcttatac ttttattata
     3361 acccattttg taaaaatata gaatattaag taaataatgt taaaattata attttgtata
     3421 taagacatta cttgattgca ataattttat agcatattca tctattctat atccagttct
     3481 ataatatata tgtttaattc cagcttgaat tatagcctta gtacaattta agcatggaaa
     3541 gtgagttaca taaatagagc aatttttaac tgaaatacct tcttttgcac aataataaag
     3601 ggcattttgt tcagcgtgaa tggttctaat acaatgtcca tctctcattt catgaccaac
     3661 ttcactacaa tgcttatctc cagaaatact accattatat ccagtagaca ctattctgtt
     3721 ttctgaattg acaattatag ctcctacata agctctatca caagttcctc tttcagctac
     3781 tgtttcgcac agtctcataa aatattcttg ccatgagcat ctattttcaa gttttttatt
     3841 caaatcatat acctccaaaa tttatatcta atatataaga tttatacctt aaaaataata
     3901 ttaaaaacaa atttatatga taaataaaat ttattaatac tgtttttagt ctaaattatt
     3961 tcattttaaa ttaaagttac aaggaatcta agattatata tatctttctt cgtaatatga
     4021 atgttacaat ataaaaaact gtctcaataa aatagagaca gttttacagt caaagtttat
     4081 aatacaagtt catatttttc tttaaattct tttacagctt cgggccatgc cttttgttct
     4141 gtaatacctt tttctttagc tattttaatt gcaatctctc ttatcattgc agcgccttct
     4201 aatattccca tttttaatct cccttcaaat atctttttgt atagaatata aatagtgcta
     4261 agatttatta catgctgcat atacaatatc tatacaacac ataacttgta aattcctttt
     4321 attttcatac gtctttaacc gacatataat tatattcaat aagttttaaa attatgaact
     4381 caattataat tatttttatt tttttagaat ctaaacacta aaaattgtag gtaaatttgt
     4441 cttatgcctt tataaatatt tctacagttt taattactgg acatatctat ttttattata
     4501 aaaagcgatt aaaaatggag aatatcctca taaaaactat atataagcaa aacggttaaa
     4561 ggaaaaagaa gaaatttact tgaatgttaa aatgcgttgc ttgaagcaat gggcagtttt
     4621 gcctataata tcggtaacaa ctgaaagaaa ggtggttagc cctaatgtta agcgaaaaca
     4681 ttaaaacaat caggaagtca aaaggacttt cacaacaaga acttgctata aaactgaatg
     4741 tagtgcgaca aacaatatct aaatgggaac aaggactgtc ggttcccgat tctgatatgt
     4801 tgatttccat atcagaggtg cttgaaacgc ccgtaagcac tttacttgga gaaactattg
     4861 ttgagtcgga agttgataac ttaaaagtga tttctgaaaa attagagatt ataaacttgc
     4921 aacttgcaca aagaaaaaac atgagaagaa aaatgtttca ttggttattt attttattat
     4981 gtgcagtcat attaatagtt tttgtggtct tagggagact aaatagtcct tacttggatt
     5041 ggaattatac cgaccctgaa accgctgttt ttggagtagc ttttcatgct tttgagtggc
     5101 tatttgtcag attagtaccg attgtcctta taggagcaat tattggaatc tttttgacac
     5161 gtaagaaaat atgaagtaac tctgcttttt ataatacagc aagcgttcct taaaagcgtc
     5221 attctacatg gcaatcgagg acgactatgt aaggaagaac ctttttgatt tccaactaag
     5281 cgaagttatt gaggacgaca gtaggcccaa aaccgcatta agtaaagtac aggaagcaag
     5341 actgctggac ttatactctc atgcgtcaga tgcaggaaat gcgaggtctg gtagcatgat
     5401 ttacaacgat ttgtacaacg actacgagta gaaatatatg gagataagta ttatctacga
     5461 aatcagaaaa gaaatgactt gataatttac tcatgttata attattgatg tgaaattaat
     5521 aaatcgaaat tccttttttg gatttaacaa tactgagtaa ggaaaaagtg aattactttt
     5581 gcaaggaggt gttatttgga tggtgctagc aggtattttg attgtagtac aaggcttttt
     5641 gactggtcct ttagcaaaaa aaattggtga gaagcgacta atactgatag gtatgttttg
     5701 caaagcctta gtagcgtttg ccatggtatt gacaactggg tttgttagtg tgttggtggt
     5761 ttttggctta tttgcggtat cttctgctat tacagtaccc acattaaacg caaccctttc
     5821 aaaaactgac aatcagcata aaggaacact gatgggattt gccagtaccg ccggaaatct
     5881 tagcaaagtt attgctcctt tattaactgg gtatctgtat gaaagcaata ttgaattacc
     5941 atatataacc acgggaggga tagccttaat cggagtggtt atctgctata tctggataaa
     6001 aaatcaaaag aacaaatgat ggaagaggac gtttatatgg aggttataca cagtaactaa
     6061 tctggtatag aaattcaatt gaggcttttc tgctttatta aagtcttgct actacttaac
     6121 cctataatat ccttaattca ttatgacaaa ttccaattca acagttttga tacacataag
     6181 gaggtaacaa tactatggaa aattatcaat gaagtgctaa ttctttatca gtgaagttat
     6241 ccataagttt ttctacctgt aatcgtctgg tgctttttgc cagatcattg gtaggtaaga
     6301 aaaattcatc aacagacaca tgaagtaatg atacaaggtc acttgtatgc ttggatgttg
     6361 ccctttattt tcaatattag ttaagtaacg tgggtcaata tttgtaattc gattttaggg
     6421 aaatatttaa agaaatattt tcgttttatt tctttaaatc ttcaagaata ttgatagttt
     6481 tttgaaagta ggtgtgtatt atgaagaatg agaaaaaaat tacagtcgca ttgctcattg
     6541 tttatttatt gattttatca tggattattt tatttaagct acaattttca ttttctgcgt
     6601 tagaccacat aagacaaata aacttaattc cttttggtgg ttcagttatt gcaaatggaa
     6661 aaattgactt cgatgaaatt ataaacaatg ccattgtttt tatccctgta ggtgcatatt
     6721 tcagtttgct ttttaaaaat aaatctgttc taaaagcaat aggttctgtt ttaggaatta
     6781 gctttgtata tgagataatt caatttatat ttgctatcgg agcaagtgat attacagact
     6841 taattagcaa cacattaggg ggtataattg gaattgcatt agtatatgta ttatctattg
     6901 ttctaaagga taaaacacat aagatattaa atcgaatagc aatagtatgt actgtattag
     6961 tagttgcctt cttatttatg cttcttgctg taaataatat attttaatgc catgttaatt
     7021 acatgttgtt ttggaaaaga ctttaatctg tcaaagtttg cttttgaaat tgaacatgaa
     7081 attacaccaa tggaacaaat gaaagctata aatttttcat tggagaaaga tgactgtaca
     7141 agtaaagtat taatatacaa tccagacaaa tggaaatatg tagaatacta tttctataaa
     7201 gatagaccta ttaaaaaaac tattggaaaa atttattcaa ttttgcattt gtcacaataa
     7261 aatatgtttt gaaatattga atacttaaat atgcaaaaaa acaaatgtaa ctggctatgg
     7321 ggctatatct actaaaaagc tatgtgatag gttgggatta aatttaaaca tcacttctag
     7381 aaggcatgaa tacacaaggc tgacaaacta tacagttatg attaaacgat accaacggga
     7441 ttgaaaattt cgtgaagaac tttgagaagt cctattacac atgagcaaca gcaacgaagc
     7501 tattgaagca aggacgcagg caatctagcg gttatctgac aaaggagttg taggacttga
     7561 atgtagatac tatcagaacg gatataccga ccagctctgc ggttacagat gtgattgtgt
     7621 agaatatcga gcagtcggga acggacactt tatctgctac ctacgaagta gatcagcaga
     7681 taaaagagag agaacagaca acagcggtca aggcaaccta taccgtaaaa gtccatgtgg
     7741 acgctgacgg ggatatggta atcgttcaga accttactct tgcaccagca atcgaaaaat
     7801 cagactatga gccaaagata tcagaaacag acgcaagcgt ggacgctgat accgttaatg
     7861 acgctaccgc atttctggaa acattctttg aggtgtaccc gacagccaca gaaaaagaac
     7921 ttgcctatta tgtatcggaa aatgtgattg aacctatcgg cagggactac ctttattctg
     7981 aattggtaaa cccagtcttt acaaaggacg gggacaatgt gaaagtcaag gtagctgtga
     8041 aattccttga taatcagaca aaggcaacgc aggtgtcgca gtatgatctt gtactccaaa
     8101 aggatagcaa ctggaagatt ttaggataac atggaaatgg gcttgtatca taattatagt
     8161 gtgcaagccc ttttcatatt gaagtatcaa taatcaataa ttataattag gtacatgaat
     8221 ggagggataa aatgttcaaa attggtgagt tttcaaaatt gacacaagtt tctactcgta
     8281 tgttgcgtta ttatgatgaa acgggtttat taaaacctgc taaaattgat tcattaactg
     8341 gatatcgtat gtattcagca gaacaaatct caactttaaa tagaattata tatttacgtg
     8401 atagtggctt caatgtcgct gaaattgcat tagccctcaa tttcagtaat aatgccatta
     8461 tagaacagct tgatagaaag tctattgaaa ttatggataa tatacaaaaa gaacaagaaa
     8521 aactaaagaa aattgagatt gctaaaaaag aagtgttaca tggcaaaagt gaattgtatt
     8581 ttaacataac cattaaagag ataccgagtt attgcgtttt atctttaaga aaagaaatac
     8641 caaattatta ttatgagggc gaactctgga aagaactctc tgcttttgct aaagaaaata
     8701 aaataaatat ttctgatgaa acattctcaa tatatcacaa tgaagaatat aaagaaaaag
     8761 atgttgatgt agaattatgt gccattgtaa ataaaatagg aaaagctaca ggagatttta
     8821 agtttcgttt taccgaacca gtttctatga tggcttgtac tatggtatat ggagattttt
     8881 ctaatatttc ttatgcttat cgttcatttg caaaatggct tcaagaaaac agtcaatata
     8941 agatgaaagg ttcaagccgt caaatagttc atcgtggacc gtggaacgag aaaaatccag
     9001 agaactatct aacagaaatc caaattcctt tagaaaatct cttgtaaaaa atgataatct
     9061 gccttgactt taacatggtg ttaaggttta tcgtttattt atcaagaaga aaacggagga
     9121 aaatataatg acaacttata aagtaaattc aataggtact atctgcaaca atgaaaaagg
     9181 tgctttcatt caactagaag caccatatat tctagcatta agggcattag acggattcag
     9241 tcatataaat gttttatggt ggttcagtga ttttgataat acgaatttta gaagtgtatt
     9301 ggaaacagaa cagccttata aaaatgctcc agagaaaatg gggatttttg caacaaggtc
     9361 gccagtacgc ccaaatccga ttgctttatc agcagtagaa attatcgata ttgattatat
     9421 gagtgggctg atacaaattg cttatattga cgctaatgac aaaacgccta tcttggatat
     9481 taaaccatac acgccgagtt tggatagagt ggaaaatccg caagttccag attggtgtag
     9541 tcattggcct cacagcttag aagaatctgc ttcttttgca tgggaaagcg aattcaattt
     9601 ttaaggagtg atgaaccatg agtttgcagg actacatttt tgaaaaacca gtaattgaaa
     9661 cagaacatct tattttaaga acaatgaata aaaatgacat agatgatttg aaagaatgga
     9721 ttagtgatat ttctttatac gagtattggg ggaaacgtcc ctcaaaaagc gaaaaaaatc
     9781 cagaattact ctttgataag tcaaaaaaat ctactaaaag ttttcattgg ggtattgtcc
     9841 ataaacaaga tagcaaagtg attggtgaaa tatggatata tcttattgaa aataatagaa
     9901 tggcaaaagt ggctttcaga ctttctaagc attatcaagg taatggacta atgagtgaag
     9961 tgttgaaaaa cgtaattgaa ttttgttttt caaaaacaga attacaaaaa atatggacag
    10021 atgtgcatat tttaaatcat gcttcttaca agacattgga gaaagtcggt tttcaaaggg
    10081 aaggacatat tcgagggggg aaaatggtaa atacctattg cgactactac ctatatggaa
    10141 ttttaaaatc tgattatatc actaggtggt gctatggcta atattgaagc gacgattggc
    10201 gaagcaatga acaatacagt tccaaaggtc tatgatacga aaagcattaa aagatatgtg
    10261 atgaatttat tagatatggc tcattaaaaa gtaccaaaga aataaaagtt accatataga
    10321 caaaattggt tttttatatc ttttgatttc ttataagata atggcattgt gcgacaagct
    10381 atgcaaaaaa ttttcatgta tactttgatt ataatttata caaggaggta ctgatatgta
    10441 cgcatatgaa aatattgaaa tgtcattaaa ctatattgaa caacatttaa gtgagaaaat
    10501 cgaaacagag aaactggcgg aaattgcctg tctttctaca ttttattacc aacgcttgtt
    10561 taaaaggtta gttaagaaat cagttcaaga gtatatcaaa cttcgccgtt tagcaatggt
    10621 aatagcagaa ttgaataatt cagaggaaag aatattagac atagctttaa aatatggatt
    10681 ttcagaccat gctaacttca caagagcatt taaagaagtt taccatatta ctcccgatga
    10741 atacagaaag aatgttccta tgttaaatac atttgacaag ccagaacttt cttcacgtta
    10801 tatcatgatt gatgaaaatg tcccattgat tgtaggaaat attgttttgg aaattcaaag
    10861 aaaaacatta agaacaaagg aaacatattt tggttttgaa aaaacagtaa atatttctga
    10921 acaaattcct gctggagaaa gtacaggaat tgatgttcca ggtcagctat ggcgcgaatt
    10981 ccatgcaaag aaactgcaaa ccgaaaatgc catttttaac aatgatattg aacttggtgt
    11041 atctcactta gcaagtccag ataaaggaac attcctttac tttgcaggag gtatggtttc
    11101 atcaacagat gtatgtccta aaaatatggt acaacataca ttaccagcag gtgagtatat
    11161 tgtatgcaaa attgaggcag aatcttttga agaacttgtt actactgctt taaatcaagc
    11221 aaataagtat ctgtttgaaa cttggcttcc aaatcataat ttggttacaa agccattcat
    11281 ggcagaaaaa tattacaaag gaaatgcaga gattaattat atggaaatat ggataattcc
    11341 tgtgtaaaca gtaaatgcta aataggaatt atctgacgta ataaaaagga aatagaaagc
    11401 ttataaagga gggcatagtc cgttgtgata aacataaaag atataaatta tgtacctaat
    11461 attagtgaaa taagcgttta tattgaaaac tctgcatttg atgagttgta tcagcatatg
    11521 aacaatgaat ataaagcagt ttgcaaaatc gaatacagta aagatgtctg ggcacgaggt
    11581 tggaacgtaa agtttaaaaa atctgggaaa tcattgtgtg ttgtctatcc gaagaagaac
    11641 tactttactg tattggtggt tgtaggtaat aaagagaaag aagatgttga aaacttgtta
    11701 cctaggcttt ctgaatatgt acaggaaatt tatcgtaaca ctagagaggg aaatggacaa
    11761 aggtggctta tgatagactt aaaatctaaa aataatgtat atcaagatgt attaaggctt
    11821 attgaaattc gttatgccat aaaatgtgta aaaccgtaat aaaaatattt ttcacatacg
    11881 taatctcaag gtattatcgt tgagacaagt aacctatata attatgggaa atgagatttt
    11941 tagatactta agaaagtact ttagggggct tgcgttatgg aaagatttaa gtacaattcc
    12001 acacaatcaa atacaatcta ataaaacttt actaaaaatt gaatgcaata cagctttaaa
    12061 ggctcgtaca aactatatgt tttgtgcggg cttttttcat ttaataggaa tttggattcg
    12121 tcagagagta tattataaaa ataaaacgat aagggtacct taaaaataag gtacctcagg
    12181 ctgtagacaa agtcccggac tctcgtctgg gcttttctta tatttgagtt atccacaatt
    12241 caacttttta agagttatct tattaagaaa aaagtgtatg ttttccaccc tatagtttta
    12301 cttctgtgat atttgcagat gccactcaag tagaaagtgg tggtacggca gaagatgtca
    12361 tgcagagttc agaaagtctg ggactaccgc ctaattcctt agacacggaa agctcaatca
    12421 agcaggggtg taagtatttt gcgtccctgc tttcttcctg caaaaatcaa ggtatcgacg
    12481 atttgaatgt agcgatacaa tcttacaact atggcggggg ttatgtgggc tatgtggcag
    12541 gaaaagggaa aaagcacact tttaatcttg cagagagctt cgcccgtgag aaatcgggtg
    12601 gaaagaaagt aacctacgcc aacccgatag ccgttgcaaa gaacgggggc tggcggtatg
    12661 gctatggaaa tatgttttat gtggaattag tcaatcagta tttaactgtt ccgcaggtat
    12721 cgggagagct ggcacaaaag gtaatgaatg aagcgttgaa gtatcaaggg tggaaatatg
    12781 tgtttggggg aagcaatccc aacacttcct ttgattgtag cggacttgtg caatggtgct
    12841 atggaaaagc tggtatctac ttaccgagaa cggcacaaac acagtatgac gcaacccagc
    12901 atatcccttt atcgcaggca aaagctgggg agttgttctt tttccattca acctataacg
    12961 ctggttcgta tgtaac
//
LOCUS       DBGJFU010000110        12431 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.110, whole genome shotgun sequence.
ACCESSION   DBGJFU010000110 DBGJFU010000000
VERSION     DBGJFU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.110"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            99..887
                     /locus_tag="ACOIAS_002902"
     CDS             99..887
                     /locus_tag="ACOIAS_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HGZ2702596.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLTFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            908..1648
                     /locus_tag="ACOIAS_002903"
     CDS             908..1648
                     /locus_tag="ACOIAS_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGZ2702597.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            1824..2198
                     /locus_tag="ACOIAS_002904"
     CDS             1824..2198
                     /locus_tag="ACOIAS_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702598.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            2290..3750
                     /locus_tag="ACOIAS_002905"
     CDS             2290..3750
                     /locus_tag="ACOIAS_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGZ2702599.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            3803..6832
                     /locus_tag="ACOIAS_002906"
     CDS             3803..6832
                     /locus_tag="ACOIAS_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2702600.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            6909..8975
                     /locus_tag="ACOIAS_002907"
     CDS             6909..8975
                     /locus_tag="ACOIAS_002907"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGZ2702601.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    9358..9568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9794..10609
                     /locus_tag="ACOIAS_002908"
     CDS             9794..10609
                     /locus_tag="ACOIAS_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2702602.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            10637..11314
                     /locus_tag="ACOIAS_002909"
     CDS             10637..11314
                     /locus_tag="ACOIAS_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGZ2702603.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            11373..12095
                     /locus_tag="ACOIAS_002910"
     CDS             11373..12095
                     /locus_tag="ACOIAS_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702604.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         5035 a         1211 c         2243 g         3942 t
ORIGIN      
        1 cttctccgcc aattattaaa ggctattttt attaaagata gtctttttat aatgtcataa
       61 atgattagat atgtataact aaagaaagga gggaatagat gacaacttta atttttttca
      121 tagtggggat agcatctttg gtagttgctt ttgttattga tgggggacat gttggagcat
      181 tatttgtatt ttcatcagca cttgttgtgt ttggtggaac tataggagct gttggagctt
      241 cgactccatt ccctgtgttt aaaaagtcta taaagataat gttattaact tttaaaaata
      301 aaaagcctaa taacatagaa aatataatct atttcaaaga aattgcaaca aaggctagga
      361 aagaggggct tcttagtatt gacaaagaaa taagtgagaa tgatgaatta gaccctttta
      421 ttagaaaagg gcttgaactt atggtagatg gagtggatgc agttactata agaaatatat
      481 tggaatcaca gatacagata acatcaaaac gacataaaga aggaatagca atttttgatt
      541 cagcaggtgg atatgcacct acactgggga taataggtac agttatggga ttagtacata
      601 ttttaggtaa tctttcaagt aatccagatg cattaggacc tcaaatttct gttgcatttt
      661 tggctacttt gtatggtgtt ggttctgcca atttaatatg gttaccaata gcatcaaaac
      721 ttaaggctat aaatcaacaa gaagtaagtg aaagactttt ggtgatagaa gcaatattat
      781 ccatacttga gggtgataat tctaatttaa tgacagaaaa attaaaaata tttttaaatt
      841 caaaagaact aattgaactt gaagaaattg gtggtagagc tgaataatat aaaaggatgg
      901 aatgtttatg ttgtatgatg aagatgaaaa agaagaaaac aatgagcgat ggcttttaac
      961 ttattcagac ctcataacat tacttatgat tttctttgta ataatgtatt ctatgagtaa
     1021 tgttgatgcg gagaaatata aacaattatc tcaatctcta aattctgctt ttggtggttc
     1081 ttctggagtt attgaaggtg gtaatagcaa gatagaacca gtagttgaac caggaagtaa
     1141 tgatttagat actttacaaa atgctaagtt taaaaaaatt ggtgaagaaa ttcagaagta
     1201 tttaaacgaa aatggtatgg ctaattcagt atcattaaga gttcaagata gaggattagt
     1261 aataagcctt aaagatacaa tactatttga tacagggaaa gctattgtga aggataattc
     1321 tagagataag attattcaaa taggtaagat gttaaatgaa atgaatagtt acatgagagt
     1381 agaaggccat acagacaata tgtctataaa aaattcagag tttaagtcaa attgggattt
     1441 atcagttatg agagcaacca atgttgttca acttcttatt gacaatgcag gtatagcacc
     1501 agataaatta tctgctgtag gatatggtga gtttagacca atagcagaaa attcatctga
     1561 agaaggtagg tcaaaaaata gaagagttga tatagtttta gttgatagta aatatgataa
     1621 tgtagaaaat gtttcaaagg ataaatagtt aaattagctg tctcaataca gaattaattc
     1681 taaaggagat ggctttttta tgcgaaaatc aaaaatagat acatagtaag ttaccttttt
     1741 gtaggaatat atggtatctt aaatatataa tatttaaata atgtataagc tcaattaaaa
     1801 aatttataga ggatggaaaa taaatgggaa ataaaaataa aaatactaaa tataaagata
     1861 aaacaagaga aaggtataat aaaaaagttg agaatttaaa taaagaaaaa ttaaataaaa
     1921 ttaataagca atctaagaaa gtagataaga ttgatgatat tattaataaa tctagtattg
     1981 tattatgtgt acttctagta gtgttttcat tgctttactt atttaaactg gttcaactta
     2041 atataataat gatactttta gtaattacag tagctattca acaaggattg gtgtcatttg
     2101 gtgcatttag aaataaaaat tatgtaaaag ctttattttc tatatttgta atagtatgtt
     2161 tcttaattgc ttttaaatat gttgtaaatc ctgtttaaat atatcaataa acgttctata
     2221 attaatataa atagtgaagt tacagtttta atgctattat aaattttata atttgaagga
     2281 gtagatttta tgaataggtg taagtttgca aaattaatta tcacatcaat gattgtttta
     2341 ttacttacag gatgtagttt aaagaaggaa gataattata agttagaccc agaaaatccg
     2401 acatcaatac aaatttggca ttattataat ggacagcaga aaatggcttt tgataagctt
     2461 gttaaggaat ttaatgacac tgtaggtgct gaaaaaggaa ttattgttga agcattcagt
     2521 caaggtaatg tagttgattt atcagagaaa gttttagatt ctgcaaataa aaaagttggt
     2581 tctgaagatg taccaaatat atttgcagca tactcagata ctgcatatga aatggataaa
     2641 ttagggttac tagtagatat ggataagtac ataagtaaag aagaattaaa tgaatacata
     2701 gacgaatatg tagaagaagg cagaataaat tctaaggatg agtttaaact tttcccaatt
     2761 gcaaaatcaa ctgaaatttt gatgttaaat aaaactgatt gggataaatt tgcaaaagct
     2821 acaaattcaa atataaatga tataaaaaca attgaaggat tagtagagac atcaaaaaaa
     2881 tattatgaat ggacagatag tttaacgaaa gagcctaatg atggtaaagc attttttgga
     2941 agagatgctt tagcaaatta tattattata ggaagtaaac aactaggagt agaaattttt
     3001 tctgttaaag atggtaaagt tacactaaat attgatgaaa aagttatgaa aaaactatgg
     3061 gataatttct atgtaccata tataaatgga tattttgctt cttatggtaa gtttcgttct
     3121 gatgatgcaa aaacaggtga tatattagct cttgtaggtt ctagttcagg agcagcatac
     3181 tttccagata gagtaatgct aaatgataat gagagttata atattgaaac atctgttttt
     3241 caagcaccac gttttaaagg agaaggaaaa tatgctgtac aacaaggtgc aggaatggct
     3301 gtaacaaaat ctactaaaga agaagaatat gcatcagtag aatttttaaa atggtttaca
     3361 gataaagaac ctaatataaa attctctatt gcttctggat acttaccagt aaagaaaaaa
     3421 acgaatgatt tggatgttat aaaagaagtt atgaagaaag ataatttgag tttatctgat
     3481 aaagttgaag gttcaatagc ctcatctatt gaacagatta acagttgtaa tttatatact
     3541 aataaagcat ttgatggtgg aactaaagct agacaaatac ttgaatattc aatgcttgat
     3601 aaagctaaag aagattcaaa taaaataaat gaattagtaa aaagtggaac ttctaagaag
     3661 gatgcagtag cacaatttaa taatgataaa aattttaaaa cttggtttga aagttttaag
     3721 gctgagcttg aaaatgctat aaaaaaatag atgaattggc agttagtgtg ttgacgagca
     3781 caaaggtata ggtggaatgg gtatggaaaa caatctaaga aaaaacaatt caataatgaa
     3841 aaagatgata tttcccctaa ttattgttat gctaattcaa acaagtttat tttgtgccac
     3901 aattttatgg ggaggaacta ttaagaaatt aaatgataat tcgtttgaca tcttaaatga
     3961 aagagtaatt aatcgtaaaa attatataca aaatgaaatg ttacaaagat ggtcaaatac
     4021 aagtgaaacg gaagctacaa ttaattcctc tgtaaaaaaa gtattaaatg aaaaaaatgc
     4081 cttaattgag gatgtaggca acaatgaaga aattaacaaa gatattataa aaagcatatc
     4141 aaatgattta atatatcttt tgagaaaaaa ttctgttaca ggagctttta taatcctaag
     4201 tgacaaaaat attaacgata agtcaataga tagtaaaggt gaaataagta ggacaggatt
     4261 gtatataagg gatttagacc ctaagtttaa tccaaatgat aattctgatt tgttaattga
     4321 gagaggttca tcagatatag caagaagttt tggaatatct atggacagtt attgggaacc
     4381 taagttttca tttaaaagtg aaaatgagca agatggagat gatttttttt ataaaccatt
     4441 tagagctgca attgataatc caaatgtatc aagttctaat ttaggatact ggggaagtaa
     4501 gtttttattg acaaatgcag gtgataggct tatgacatat acagttcccc ttatttatga
     4561 agatggaact gtatatggag tacttggaat agatttaagt gtaaattatt taagagaatt
     4621 acttccatac tcagagataa atggaaataa aaagggtaat tatattttag gtgtagatag
     4681 aaataatgat atggcctttg aaaatgtaat ttcaaatgga tactcattaa aagaaaaatt
     4741 ttgggatggt ttacattcta atttagagca gaaggaatcg tataaaaata tatataagat
     4801 taaaaataaa gataatgaat gtggtaatgg caacatttat gctagtgtac aatacttgga
     4861 tttatataac tccaatactc catttgaaaa tgaacgttgg gctcttttgg gtattattga
     4921 agaaaaaagt cttttgtcat tttcaaaaag ggtagagaaa ttagttataa tatctatagc
     4981 aatttcttta ttaataggtc taataggtat ttacattgca tcaagattat ttacaaaacc
     5041 aattgttctt ttagccaaca aagttagaaa cagtaatcca tcaaaaccag taaatcttga
     5101 taagataaat atatctgaaa tagatgaatt atcttcagct atagagttgc tcagtgcaaa
     5161 tgttgcagat tcttcgtcaa aattatcaca gattattgaa atggtagata tgccaattgg
     5221 ggcttttgag ttatacaaaa acgcagagaa tgttttttgt acaaaagaat tttttagcgt
     5281 gtttactact gaaatcaaat ttgaagaata tggatatatg gaaaaaaata agttcctgaa
     5341 aaaattaagg ggactagata aatatttgca agaaagttat gaaacagaaa atacatatat
     5401 atataagttt gaatctaaag ataaaaaact taagtggata agaatgaata tctttgaaga
     5461 tgagctaaag gttcttggtg ttgttgttga tgtcacaaaa gaatttattg agaaaaggaa
     5521 gatagaatat gaaagagatt ttgatatttt aacgaatctt ttaaatagga gagcttttca
     5581 ttcacagata caagaaaaat ttaagaataa agatgaactt aagatagcag catttattat
     5641 gtgggattta gataatctaa aatatattaa tgatacatat ggacatgatg ctggtgatga
     5701 atatattagg tgtgctgcag acatacttaa gaagtttata tcatggaatg gtatagtatc
     5761 tagactttca ggtgatgagt tttatgtatt tatatatgga tatgaaagca aagatagtat
     5821 aagagatatt attaattctg ttaagaaaca gatgaataat accttattaa aacttcatga
     5881 tggaacagaa tttaaaataa gggcatcagc aggaatttcc tggtaccctg atgactcaac
     5941 caactatgat gaattaatga gatacgcaga ttttgctatg tatcaaatta aaaatacaat
     6001 caagggtgaa gttagtgaat ttgatattaa tatatataat aaggattcat tcttattgag
     6061 taatagagaa gaattaaaca agttaattga tggtcaatta gttgagtatg catttcagcc
     6121 tattgtagat tgtaaaggta aagtatttgc atatgaagca cttatgagac ctaaaataaa
     6181 aacttttagt tcaccaatag atgtcattag attagctcgt tcacagtcta aattatatca
     6241 aattgagcgt attacatggt ttaaagcact taaatcattt aaaaaataca aagaaaaatt
     6301 tggcgattgc aaattgttct taaattctat tcctaataat aatttgtcaa aagtagattt
     6361 tgaggaaatt gaagatttat atggtgaata tttaaataga gtagtactag aaataacaga
     6421 gaatgataag attaatgaag attttattga aaaaaagaaa gcatatatag accattggaa
     6481 tatagaaatt gctttagatg attttgggac aggttacaac ggagatgctg tactattata
     6541 tataatgcca gactatgtaa agattgatat gtctattgta agaggtattg ataaagatga
     6601 aaacagacag aaaatattaa aaaatcttat ctcatattct aacgaaagaa atattaaagt
     6661 gatagcagaa ggtgtagaaa caaaaggtga aatggaaaca ctaattaatc ttggagtaga
     6721 ttatatgcaa ggctactata ttagtaaacc atcatttata cctgaagata taagcgacaa
     6781 aattaaaaaa gaactcaaga tatgtcatga aaataggaat gatgtggttt aatagaggaa
     6841 aaattaaatt tatttttatg aacttttagt ttataattaa aaagaaaata atataattat
     6901 aaatcaaggt ggtggagcta ataatcacaa caaatataga tataagaaag ttaaatgaaa
     6961 atcaattaga agcagttgag catatagatg ggccttgcat gatacttgca gggcctggtt
     7021 caggtaagac cagagtaatt acatatagaa tagccaatat ggttgtaaac aaaaacatag
     7081 caccaacaag aatcttggca ataagtttta ccaaggcatc ttcaatagaa atgaaaaata
     7141 gagctttgaa tttaagtgat gacataagac ttaataaggt aacctatggt acttttcact
     7201 ctgtattttt taaaattcta agatactttg aaagatacaa cttagacagt atatttgatg
     7261 aaaagtcaaa gcgaatgaca atcaaggcaa tcctaaaaag tttaaatata gagaatgctg
     7321 atgatgatga gaatatagga caagttataa atgaaatttc ctatgtaaaa aatgaactta
     7381 tggataaaaa tgaatttaat tcagaagtat taacaaaaga tgagttttta aaagtataca
     7441 acttatacga agaacaaaaa tctaaagtta ataaaataga ttttgatgat atgcttataa
     7501 gaacttatta tttacttttg aacaataagt ctgcactaga aatggttaga aatgtgtata
     7561 aatatatatt gatagatgaa tttcaagata taaataaagt acagtttgaa gttttaaagt
     7621 taatttgtag cccattaaac aatatatttg cagttggaga tgaagaccaa agtatatatg
     7681 gatttagagg tgctagacca gattttttgc ttgaatttga acaatacttt aataatacta
     7741 aaaaaataat attagatata aattacaggt ctaaaagtga gatagtgcat actgcaaata
     7801 gacttatcga caaaaataaa aatagatatg aaaaaatcat taagtgtagt cagggggatg
     7861 gaggaagtgt tacctatatt tctccacatg attcagagga agaagcctta tatattgcca
     7921 gggaaataat tgacgaaata caaaaagatt atgtagagta ttcagatttt gcagtaattt
     7981 atagaactaa tatacagtca agagcattag tagatgtatt tatggatatg aggattccat
     8041 ttgtagtaaa agactcagta attacaatat atgaccattg ggcaagtcaa gatatactag
     8101 cctatttaag aataggtatt aatccaaaat caaataaaga ttggttgaga attataaaca
     8161 agccttttag atatatatcc aaagacagta taaatatggt taaggatgaa aaagacttta
     8221 taacagcatt aataaataag tgtaatcttc atcctaaaca agttaaaaca ataaatgatt
     8281 tggaaataga tttaagttat ttaaatactt taaatcctaa aaatgcaata tcttatatta
     8341 gaactagtct tgattatgat agatatgttt tagattattg tagcaacaga aagataaaga
     8401 ctaatggact tgtagagata ttaaatgaac ttgagagttc agcaactaat tttaatactg
     8461 ttacagagtt tttagagcat attgatagag taaagactga gttaacggaa aataacaaga
     8521 ataagcagac tgagggtgtc atatttacaa ctatgcatag tgcaaaagga ttagaattta
     8581 gaaatgttta tattataggt gtaaatgaag gaacaatacc tcatgaaaaa tcatatgata
     8641 tttgtaagga agagaaaaaa gaagaacaat tagaagaaga aagaagactt atgtatgttg
     8701 ctattacaag agctgaagaa aagttatgta taagctctac tttaaataag tatggtaaga
     8761 aagttgataa atcattattt attaatgata taaaatctcc aacgaaaaaa gaaattgaca
     8821 gtattggtgt aggggataag gtataccata aaaaatttca tgaaggagaa ataattaaaa
     8881 aagatggaat tatgtttaca ataaagttta aagatagaga gcgaatcttg gatttgaaaa
     8941 cttgtttatt gaagaatata atttatacta tttaatttag aatctaaatt tagatagaaa
     9001 tataatgtat tatttattat gtgcaagaca aatagatatg tgtaaaatgt attatttaac
     9061 ttattagaaa aagaaataag ggtttaatat attatttata gttgtttaga aaaagcaaca
     9121 atgtggaaaa tattatttag tattattaaa gaaaagcaac aatgtgaaag atattattta
     9181 gtattattta agaaatagct aaaaatatga aatatactat ttataattac acaaataata
     9241 tcaataaata taaacagtag tatttagata atttacaagt aaaaattatt aaaaaaataa
     9301 aaaacttatt gacaaaaata ttataaggaa gtacaattgt attaacaatt taaggaaaca
     9361 aatccaatga tagagaaagt agcatatatg gatttacagc gagttagggg ttggtgtaag
     9421 cctaacaatg aagtctatgt gaagagagct ctggaggaga ttatcagaaa ctttagtatg
     9481 ataattggtt tacccgcctt aagggattga gaggataagt acttacttat caaaaagagt
     9541 ggtaacgcgg atataattcg tctcttagct gtaaagctaa gggacttttt tgattttaaa
     9601 ataaaaatat ccatacagct taaaagctaa cagtcacaaa ttttgaaagt aatataagta
     9661 tttatcaata agagtggtaa cgcggtaaag tcgtctctta gtcattaaag actgagagac
     9721 tttttttgta actcgaaaca tttaagtatt aaaatctgag ggaaattata attaaaagag
     9781 aggggtagtt aaaatgaaag tatttaaaaa attattaagt ttaggattag tattaggatt
     9841 gacattatcg ctagtaggat gtagtggtgg aggagaaaaa acaaagttag aacaaataaa
     9901 agacaatgga aaattagttg ttggtactag tgcagaattt cctccatttg aatttcataa
     9961 ggtagtagat ggaaaagatt caataaaagg attcgatata atgttggctg aagaatttgc
    10021 aaaagagctt ggtgtaaagg ttgaaattaa ggatatgtca tttgatggat taataggtgc
    10081 tttaaatgca gaccaagttg atattgttct tgcaggtatg tctccaacac cagagagaga
    10141 aaagagtgtt gatttttcag aattatatta tttaagtaga aatgcagtta tagttaaaga
    10201 tgcagatata gacaaagtaa aaactgaaga tgatttaaag aaacttagag ttggagttca
    10261 agcaggaagt atccaagaag aatatgtagt taacacttta aaaatgacaa ctacaaaatc
    10321 tttgaaagca atacctgatt taattacaga attaaaaaat ggaaacatag atgcagttgt
    10381 tactaatgaa gcagtttctt taataaatgt taaaaaatat gatggaataa aaatggcaaa
    10441 tactgaagtt gggaaagatg taacagaagg tatggcagca gcaatcaaaa aatctgacaa
    10501 taacaaagat tttatagaat tattaaacaa aaagataaaa gaattacaag atggcaagaa
    10561 gatagaagaa tttttaaatg aagcatctac tgaagctgct tcaaattagt aagaaagtta
    10621 tgtgggagat gaaattatga gtttagattt tagtttttta agtagatttg ggacttcttt
    10681 tttggaagga acaggtgtga cagtatcaat ttctcttgtg gcattatgct ttggatttat
    10741 aataggtata attatctgta tggcaaaaat atcaaagagc aaagtattaa gagcaatatc
    10801 atcaatctat attgaggttt taagagggac accattatta gtacagatat atatagtatg
    10861 gtttggttta cctcaattag gaataagatt ccctatgttg tttggtatac caagtgaatt
    10921 tatagctagt gcatttgcat tatcagtaaa ctctggtgca tacgtggcag agatacttag
    10981 atcaggtata caatcagttg ataatggaca aatggaagca agtagatcat taggattaaa
    11041 ttattggtca actatgagat atataataat acctcaagct ataaaaaata tattaccttc
    11101 attagcaaat gagtttatta cacttgtaaa agaatcttca ataatatctg taataggagt
    11161 tgttgagata atgcgtactg ctgatatagt aaaaaatgca gcatttagag cattagagcc
    11221 attaatagta gcagcagcaa tttactttgt tataacattt actctatcaa gattagttgg
    11281 attattagaa aagaaactat ctgtgtctaa ctagatgaga gcatgtttag catttaggga
    11341 aaacatagaa tagtaaaaag gaaagtgata atatgattac aataaaaaat ttaagtaaat
    11401 cttttggaga tttaaatgta ttaaaaaata tagacttaga aatcgctaaa ggcgaaataa
    11461 tggttatagt aggtccaagt ggttctggta aaagtacact tcttagatgt atgaatttac
    11521 tagaaatacc tacaggtgga gaaataatat ttgaaggaaa aaatttggtc gataaaaaaa
    11581 ctaatattga tgaggttaga caaaatatag gtatggtgtt tcagaacttt aatttattcc
    11641 cacataagac tattttagat aatataacat tagcaccaat taaattaaaa aagatgacta
    11701 aggaagaagc agaaaaaaaa gcagaaattt tgttgagtag agttggtctt ttagataaaa
    11761 aagattcata tccatcacaa ttatctggag gacaaaagca aagaatagca atagctagag
    11821 cacttgctat ggaaccagat atgatgttgt ttgatgagcc aacatctgct cttgacccag
    11881 aaatggtaaa cgaagttttg gatgttataa aagaacttgc taaagaaggt atgacaatgg
    11941 caattgtaac tcatgagatg ggatttgcta aggaagttgc agatagagtt atttttatag
    12001 atggaggaag tatacttgaa gataatactc cagaagaagt tttcggaaat ccaaaacatg
    12061 aaagaacaaa agcgttttta gcaaaagtgc tataatatat aaaaaggatg ttattgatat
    12121 tagaaattca atacatcctt ttttagtgat ggttaatatt aattttaaaa ttaaagttat
    12181 attaatatta actttatttt tacgattttt tgcagaaaaa aaaagagctc ggggtaagct
    12241 ctttttttcc accgaattaa tttccgttac taatccgatt tttaataaat ataaaatata
    12301 tccttatgaa agggtattgg gaatagatat attaactaac tagggttacc agggggataa
    12361 ccttaattta ttataacaaa atgcgacaca aaaatcaata ttttttttga aataaattat
    12421 aaaagttatt t
//
LOCUS       DBGJFU010000111        12327 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.111, whole genome shotgun sequence.
ACCESSION   DBGJFU010000111 DBGJFU010000000
VERSION     DBGJFU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.111"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            309..671
                     /locus_tag="ACOIAS_002911"
     CDS             309..671
                     /locus_tag="ACOIAS_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2702605.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            769..1353
                     /locus_tag="ACOIAS_002912"
     CDS             769..1353
                     /locus_tag="ACOIAS_002912"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HGZ2702606.1"
                     /translation="MDALREIGNIGSGNAITALASMINSNVEVLIPMVKILEYNEATN
                     LLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAFLDEIEAIEISA
                     IKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRFSDVGDKVLFIE
                     NKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1350..1835
                     /locus_tag="ACOIAS_002913"
     CDS             1350..1835
                     /locus_tag="ACOIAS_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HGZ2702607.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            1878..2396
                     /locus_tag="ACOIAS_002914"
     CDS             1878..2396
                     /locus_tag="ACOIAS_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGZ2702608.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2532..3491
                     /locus_tag="ACOIAS_002915"
     CDS             2532..3491
                     /locus_tag="ACOIAS_002915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGZ2702609.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3517..5220
                     /locus_tag="ACOIAS_002916"
     CDS             3517..5220
                     /locus_tag="ACOIAS_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGZ2702610.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5481..7583
                     /locus_tag="ACOIAS_002917"
     CDS             5481..7583
                     /locus_tag="ACOIAS_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGZ2702611.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7596..8036
                     /locus_tag="ACOIAS_002918"
     CDS             7596..8036
                     /locus_tag="ACOIAS_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGZ2702612.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            8072..8875
                     /locus_tag="ACOIAS_002919"
     CDS             8072..8875
                     /locus_tag="ACOIAS_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGZ2702613.1"
                     /translation="MIKLTDEEFIVLVNYVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            8915..9511
                     /locus_tag="ACOIAS_002920"
     CDS             8915..9511
                     /locus_tag="ACOIAS_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HGZ2702614.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9617..10480
                     /locus_tag="ACOIAS_002921"
     CDS             9617..10480
                     /locus_tag="ACOIAS_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HGZ2702615.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10547..11536
                     /locus_tag="ACOIAS_002922"
     CDS             10547..11536
                     /locus_tag="ACOIAS_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HGZ2702616.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYIEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11628..12239
                     /locus_tag="ACOIAS_002923"
     CDS             11628..12239
                     /locus_tag="ACOIAS_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2702617.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         4984 a         1212 c         2314 g         3817 t
ORIGIN      
        1 tttttttata caaacataaa aaaggtataa agttgtttta ttataattta attacttttg
       61 ataacttttt atgttttata caattatatt tttaataact cgtcaaaacg ccaaacatat
      121 attacaatag atatataagt ttttttgttg acaaattatg ttaagagaat taagtataat
      181 atggcataaa taattacttt tatttatcaa tgaaatgtat ttattgtgta gcgaaaaata
      241 gtcagatagt atagacaata actgaagata aaagcgttta ttgttgtaca tatggaggag
      301 ttagttttat ggaaaataaa attttgatag tagatgatgc tatttttatg agaatgataa
      361 ttaaagaagc tctgagtaaa agtggctata ataatttaat agaagcaaat gatggagaag
      421 aagcgtgtaa tatatttaaa tatgaaaagc ctgatttgat gttgcttgat attacaatgc
      481 caaaaaaaga tggtttagag gtgctaagag aaataaaaaa gttggattca agtgctaagg
      541 ttgtaatgtg ctctgcaata ggacaagaga atatgattgt agaagccata aaacttggag
      601 cgttagattt tatagtgaag cccttcaaaa cagaaacatt agttaaagtt gtagataatg
      661 ctttaaaata aaagtgtaaa tatacactaa tacaatgaaa agcaaataaa tgcaataata
      721 aagggagggg tttatattat aaattattta gaacttgatg atgttcatat agatgctctt
      781 agagaaattg gtaatatagg ctctgggaat gcaattactg cacttgcatc catgataaat
      841 agcaatgtag aagtattaat acctatggtc aagatacttg aatataatga agcgacaaat
      901 ttgcttggtg gtccagaaaa caaagtagta tgtattttac tcgatatgaa aggtgatatc
      961 aatggaatgt ttatgttttt gttggatgaa tcaataactc aacttatgct aagttctctt
     1021 tttaataaag aagaagcttt tttagatgag attgaggcaa ttgaaatttc agcaattaag
     1081 gaaataggaa atattatggc aagttcatat gtaaatgcta ttgcatctat gttaaacatg
     1141 actataagtg tttcaattcc agatatatgt attgatatgg ttggagctgt tctaaatgta
     1201 cctatgataa gattttcaga tgttggtgac aaagtcttat ttatagaaaa taaatttaaa
     1261 atgagtgata attattttac tagtcacatt ttgatgattc cagaaatgag ctcacttaga
     1321 gaaatactcg tgaggttggg attagaagta tgaatagaga gatagtagtg aacattgcag
     1381 atatgaagat agcctataga ccaaatgttt tagttactta tgcacttggt tcttgtgtag
     1441 gagtatgttt gatagataaa gtagctggaa ttggtggaat gttacacgtt atgttgccat
     1501 atagtaaaga tgctattaac attgaaaata agtgtaaatt tgctgacaca ggaataaatg
     1561 agctcattaa atctatggag aatataggtg caaatagaat atatataaaa gcaaaaattg
     1621 ctggaggtgc tcagatgttt ttaggaagta gcaattcagt aattgcaagt ataggacata
     1681 ggaatgttga ggcagtaaaa aaaactcttt taggattaaa tattccaata ttagctgagg
     1741 acacaggtat gaattatggt agaacaatac gtttttacac tgagacgggt gaattacttg
     1801 tagattcaat aaataaaggt aagaagaaat tataatatat atttcataga aagtgtattt
     1861 taggagggag gtattaaatg agatttccag atgacccata tttagattat ggactaattg
     1921 atgaaggaat taagtatcta aagtttagag tagatgagca agactttggg gttgaacttg
     1981 aaaaggtaat agaaattact ggaaatcaag aaattatatt tattccagaa ctacctacat
     2041 attctaaagg cataataaat ctaagaggaa aaatcattcc gataatagac atgcgtttga
     2101 gacttaaaaa agatgaatta atgactacaa attgcatgta tatagtagta actgaaataa
     2161 aggacttggt agttggattt gtggttgata gagttgatga tatagtaagc ttagaaaaaa
     2221 gtaaaatatc acctccacca agagttacta tagaatattc ggattatttt gtaagtggag
     2281 ttggagaatt agaaggtaaa ataattactt tactagattt agagaaactt ttgacagata
     2341 aagatgaagt tttaatagat gaattaatca atgggtttga ttgcactatc atgtaacata
     2401 gggatgtagt tggagtgtta ttataaaaag attgtgatga gaacatttaa gaatatagtg
     2461 gtataattat taagtataaa aattttaaat cattaaaatt tgaatatatt atgtttaaga
     2521 gaggtttgta tatgaaaaat aagcaacagg gaacaatttt agtcgttgat gatagtgttc
     2581 tgatgtgtga cttaatagat aaaacactta caaaagataa ttttaaggtt aaaaaagcat
     2641 ttaatgcaat agaggcaaaa gaatttattg aagaatttat accagacatt attttgcttg
     2701 atataatttt accagataac tctggttttg agttttgtaa ggaagtaaaa tctaatgctc
     2761 gtacagcaga tatacctata atttttataa cttcaaaaaa tacagataca gacatagtaa
     2821 aaggatttgg tgtaggagca attgactata tggtgaaacc ttttagtatg acagaattaa
     2881 aagctcgtgt tatagctcat cttgaagtaa agaaatctca agataaatta aaaaagataa
     2941 ataatgaact ggaatcttct cttgaaaaat tgaatagact cgttgtaaga gattatctta
     3001 caggtcttta caataggcga catataatta atcagcttat ggagcaaaga agaattaata
     3061 aatatagtga aacatcaata tcccttatcc ttggtgatat tgatgacttt aaggttatca
     3121 atgatactta tgggcatgaa gcaggggatt tcgtgttaac agtaatatca agtataataa
     3181 aaaagaattg tagacaaatt gatactgtat caagatgggg tggagaagag tttttgataa
     3241 tgctcacaaa tactccaata gaaggtgcta agatactttc agaaagaatc cgtaaagaga
     3301 taaaggagtt tgatttcaac tataaagaac ataaaataaa gtgcactatt acattaggag
     3361 tagtagaagt aaactcaaat atatcattag aagaaaacat atccttagca gataaagcaa
     3421 tgtatgaggg taaaaactta ggaaaagatt gtagcgtagt gagtacaatg aaaggattaa
     3481 aaaagatata aacttacttt atgaaggagt acagatatga aaagagaagt aaagctttct
     3541 aaattagcaa ttgtattgct gattattata gtattggctg ttctagcagg gattttttct
     3601 tctatttcat cttataaagc atttgaaaaa gtagaaaaaa gtattaatga tatgaataat
     3661 ttgaggactc aaagtcagta tataaaagat acaactagag agataagtga tgtattaaga
     3721 agatatgtat ttacaggaga aaaaaagaat aaagatattt atgaaaaaga attatacgga
     3781 aaaacaagag aatcagcttt aaacaaatta gaaaaaattg gtcttacaaa gagcgagtca
     3841 aataaaatct tagaagcaaa agaaatatca gattctttag taccaacaga acaaagtgca
     3901 ataaaggcag tcgaaagtaa tgataagagt aaagctcaaa gtataatttt tggtagtgac
     3961 tatataaact cccaagagaa aatggattct ttaataaatg aatttcaaga ggatatagtt
     4021 acccgttcaa attccaaaat tgtagaggta aaaaaagaat tacaaagtct aatattaaga
     4081 acaatattta tactttccct tttaataata ttgacaataa tagaatatac agtaataaaa
     4141 ttaaaaatag tgattcctat taataaagta gaaaaacact tttcaagaat tgcatctggg
     4201 gacttaagaa caagtataga cgttgaagaa agtaaatctg aaatagggct attagtgagt
     4261 tctgtcaaaa agatgcaaag tatgctttta aattacatag accttataga cagtacatta
     4321 actagtattg caaaaggaga tttaagagta gagatagacc aagaatttat aggtgatttt
     4381 aaatcaatta aaatatcttt agaaagtata ataaactctt ttaatggtga tttttctgaa
     4441 ataaatttat cagcagagca agttgcaagt gcttcagaac aagttgcagc aggtgcacaa
     4501 gctctttcac agggtgctac tgaacaagct agttcagtag aagaattagc ttccacgatt
     4561 aatgagatat cagatggaat taagaagtgt gcagatagtg ctagtcttgc atgtgaggta
     4621 tctgaccaat ctgcatcaca agttggtttt ggaagtagat gtatggatga tatgatgaca
     4681 tcaatgggtg aaataagtga aaaatcgagt gaaatatcaa aaataatcaa aacaatagat
     4741 gagatagcat tccaaactaa tattcttgca ttaaatgcag ctgttgaagc agcaagagca
     4801 ggtcaatatg gtagaggatt tgcagttgta gcagatgaag ttaggtcttt ggctggtaaa
     4861 agtgcaaaag cagctcaaaa tacagctatt ctcatagaag aagctattaa aacagtagaa
     4921 aatggtgtca gaattgctaa agagactgca aatgcattgg acttggtagt aaatggtgta
     4981 gaaaaaacta ctaattatat taatggtatt tcttcggctg tacatagtca agaagtagca
     5041 attacacaga ttttatctgg aatagagcaa atttccatag tagtccaaat gaactcagca
     5101 acagcagagg aaagtgcagc agcctcagaa gagatgtctg ctcaagctca aattttaaaa
     5161 gaattagtag aaaaatttca tctaaaggaa agtgaagtaa gagaatcatc atgggtttaa
     5221 attatgaggt gaatattgaa gtaagagaat tatcatgtat ttaaattatg agatgtatat
     5281 atattgaaat aagagaatta tcatgtattt aaattatgag atgtatatat attgaagtaa
     5341 gagaataatt gtgtatttaa attatgaggt acatatagtg agataagaga atcatcaagg
     5401 atttaaatta tgagatgcat atataataga aaattatttg taggtcgtag gtgataaatg
     5461 tgaattatat tgaaccgagt atgagacaaa tgttagatat gtttatattt gagacaagtt
     5521 tattactcga acaattagat gaaattatga ttcaaataga aaaagagaaa gtatttactc
     5581 ataacaacat taatgaaact tttagaataa tgcatacgat aaaaggttca gcatctatga
     5641 tggggttaga caatatatct gctttagcac atagtgtaga agacttgttt tatgctatac
     5701 gtgaaggtaa aatagaagtt ttagacaatg acctattatt tgatttagtc tttcaatcat
     5761 tagattttat aagaagagat atagatgagt tacaatcaag aggttgtata tcttctgatt
     5821 actctaattt tattgaagga attgaagagt atgtgagtac tttggaatta tcaagttcta
     5881 tgaaatcaaa ggataatgca aatttagaaa gtaaagacga aaaagaagta atcgattcaa
     5941 attttgaata caaagaagac tctgatgtag taaaaataat ctttgaacaa ggttgcatga
     6001 tggaaaatgt gagagctttc atgcttgtat ttgaacttag agattactgt gaatttatag
     6061 atttttatcc aaaagatgta gaaacaaatg cagagtctac agagtatatt aaaagttatg
     6121 ggttttatat acaatttgta gctaaagagg ataaggcaat agtttataaa actatagaag
     6181 ataatctcta tgtaaaagaa tacataaata ttagtaaaaa agaaatagaa atgaaaaata
     6241 catcaaacaa tgatgcagta gaaaataaat taaaagataa taataaagta agtaaaagtt
     6301 atcaaaataa ctatacagaa gatgagatac aagaagatgt tttaaatgaa aaaactgata
     6361 tggaaaaaga tagtctaagt gaagaaaatg aacatttacc aataagtaag cagagtatta
     6421 tgagtgtaaa tttagaaaaa ttggacttac ttcaaaatat agttggagaa attgtaatta
     6481 tggaatcaat ggttgtaaat agcgtaaatt ctacaggtgt tgatatagat aatttcaata
     6541 aggcaacaag acaattacac aagcttacag atgaattgca agatatagta atgtctatgc
     6601 gtatgattca aattgctggt gtatttcaaa aaatgcatag aattgtacga gatatgaata
     6661 aaaaattaga taaaaatgta gagttaataa ctataggagc agagacagag gtagataaga
     6721 gtgtaattga taatttagca gaacctttta tgcaccttat aagaaatgct atggaccatg
     6781 caatagagcc tattgaggag cgtatagcga aaggtaaaga agaagtggga aagatagttt
     6841 tatctgctga aaatctaggc ggagaagctg tgataagtat ttcagatgat ggtagaggca
     6901 ttatgagaga taagattttg gcaaaagcta aggaacaagg tcttcttaaa catcaaagta
     6961 atgagtatac tgatgacgag gtcaattctt tgattatgac tccaggcttt tctacaaaag
     7021 aatctataac tgaattttct ggaagaggag taggcatgga cattgttcgt aaaagtattg
     7081 aaaaagttgg aggttcaatt gaagtaaaaa gtaaacaaga tgaagggaca acctttacta
     7141 taaaaatacc attaactctt tctatagtag agggtatgaa gtttaaagtt ggagattcac
     7201 tatttacttt accaatatca tccataagaa gaacatttaa acttacagac ttaaatcaaa
     7261 taattacaga aggaaataag acagagttaa ttacaatata tggagcatgt attcccatta
     7321 taaggttaca taaaatatat aatattcaaa caaaagtaac caatctgatg aatggtaata
     7381 tgataattat tgaaaatgag cataaagcag tatgcatttt tgttgatgaa ataataggta
     7441 atcagcaagt tgttgtgaaa ccatttccag tttattttaa ccgatttaat ataaaaaata
     7501 agggtttatc gggatgtaca atacttgggg atggaagtat aagtttaatt atagatgcag
     7561 atagcattat agaaatatat taggggattt aaagtatggc taaagatgaa attattaaac
     7621 aagtattaac attttatgtc aatgatgtta tttatggaat agaattagaa aatgtgattg
     7681 aaaccataag atttcaatca ataacgtatg taccatgtct tccaacatat ataagtggag
     7741 ttattaattt gagaggaaga ataataccag ttattaatat gcatataaag tacaatcttc
     7801 ctaaagctga ttataatgag agaacttgta taataattac aaaggttgat gagtatcaag
     7861 ttggaattat tgtagataaa gttgttgatg ttattcatat agaaaatcta aatttactag
     7921 agacgactaa ttctaacaac actaatatta ataaagatat aaaagagatt gcgaaagttg
     7981 aagataattc tatattaatt ttagatattc gaaagttttt gattaatagc atatagttag
     8041 ttctgataaa ttagcaggag gaagtttttg catgataaaa ctcacagatg aagaatttat
     8101 agttcttgta aactatgtaa aaaaagaata tggtattgat ttaagtaaaa aaagagcgtt
     8161 aattgaaggt cgcttataca atacaatgat agagaaaaaa cttagttctt ttagtcaata
     8221 tatgaatcta ttatttaaag ataagacagg aaatgaagct ataaacctaa taaatagatt
     8281 gagtactaac cacacatttt ttatgagaga acctcaacat tttgagttta tacagaatag
     8341 tatacttcct ttttgggaag aaaataataa aataagaaac ataaatatat ggagtgcagg
     8401 atgttctagt ggagaagaag cgtattcaat agctatgact ttggatgatt attttggata
     8461 taataaagag ttgtggaaca ttaaaatcat agccacagat atttcaacta actcacttga
     8521 aaaagctaaa agaggtatat acatagaatc aaatgttaac aatgttcctg agttatggaa
     8581 gaagaagtat tttatagaca acaaggatgg tacatttaaa atttgtgata agattagaaa
     8641 aagtgtaatt tttaagccgt ttaatttaat gaatgatttc tcatatcaac attttgattt
     8701 gattttttgt agaaatgtta tgatttattt tgatttaaaa acaagagaag aacttataaa
     8761 taaattttat aatgctacca aaaaagatag ttttttgttt gtaggacatg cagaagtaat
     8821 caacagaagt agtactaaat acaaatatgt aaaacctgct atatataaac gataaaaagg
     8881 tatttttgat atatgatttt ttggaggcgg taatgtggaa cgttttaata atatggttat
     8941 tgctataggc gcatctgtag gtggtacaga agcaatactt gaaattataa aagacctacc
     9001 aaaatcaaca ccagggattg ttgtagttca acatatgcca gctatattta cttatatgta
     9061 tgcacaacgg cttgacaagc agtgtattat gaatgtaaga gaagctaaga ataatgatag
     9121 agtcgaacaa ggaaatgtgt taattgcacc tgggggttac cagatgaaat tgtgtacaga
     9181 taaacaagga tattatgtaa cttgtgaaaa gggagaaaga ataagtggtc actgtccatc
     9241 agtagacgtt ttgtttgatt cagtagcaga agtagcaggt aaaaattcaa taggaataat
     9301 acttacaggt atgggttatg atggagcaaa tggactttta aaaatgaaag aaaatggttc
     9361 atttacaatt ggacaggatg aaaattcttg tatagtatat ggaatgccaa tggtagcttt
     9421 tgatagaggt tctgttatgt tacaattacc tcttggtgaa atttcaaact gtttgataag
     9481 acatattaat attttaaaac aaaaagatta attttttata aataaaaatt aatcttttat
     9541 taaattatat ggataaaaaa ccctagaaac tccaaatata ataagagaga tttatattta
     9601 ggaggaaaat gataatatga aattttttaa agtcgataca aaagctaaag ttttaagata
     9661 tgtatttgtt ttaacattga ctataatggc tgttttggca attaaacatg actataaata
     9721 taataattta aggaaagatg cttcaattaa tttaaatagt aataaggaag cagtgactgt
     9781 tatgagtaac cagtcattac caaagggtaa tacaaatagt aaacaaactt tatcagtaag
     9841 tacaatagga agtaaaacaa tagtgaaaaa tgatgagtat ttagaaagta ctataaacat
     9901 gcctgtaata acaaattcaa ataaaattgt tgaaaggtct acaaatgata agataaaaaa
     9961 tgacatattt gaattttata ataaatcata taaagaagca aagcagtatc ttaaggataa
    10021 tcctgacgag aaaaataagt ttgttgcaaa tgtagacttt gaacttaaaa agaatactga
    10081 ttctgctctt agtataaaag tgagatatta tacttatagt ggtggagcac atggatttta
    10141 tcaagatata gcatataatg tggatatgag aactggaaaa tttttagaat taatggactt
    10201 gtttaaggat aatactaaat ataaagaagt tatagatgaa gaaattaaaa gacaaatagc
    10261 tgaacttgaa aaaaaggatg aagaaaatat cggaatatat aattttaaag gtataaaaga
    10321 gaatcaaaac ttttatttgc aagatgagaa tctagttgta tattttgatt tatatgatat
    10381 aactccatat gcagcaggaa taccagaatt ttcaataaat aaagcgttga taagttctat
    10441 gctaaaacca gagtatgtag atttatttga tttgaaatag aagattaaat taaatgttat
    10501 gctataatat ttagcataga gacttattaa aattgggggt ataaaaatgc tacttttaaa
    10561 aaaagacgac ataaaaaaag tatttacaat gagagatgca attgaagctg ataaggaagc
    10621 ttttagaatt tattgtgaag gaaagagtgt aaatcctttg agaaccaata tatctgttcc
    10681 aagtcaagat gctagtatgt tatttatgcc aggatacata gaagaattag gctgtggggg
    10741 aataaaaatt gtatctgtat ttcctaaaaa tgctcaaaaa ggcaaaccag taattccagc
    10801 aacagtacta ttattagatg gagaaactgg agaagttagt gcagtattgg atggcactta
    10861 tgtaactcag attcgtactg gagcagcctc tggagcagct atagacgtat tggctaaaaa
    10921 agattctaag attggagctt tgataggtct aggaggacaa ggtgagccac aacttgaagc
    10981 catgatagaa gctagaaatt tggatgaggt aagagttttt agtagaaaca aggaaagtag
    11041 agaaaaattt gcagaagaaa tgaacttaaa attaagtaaa tataatacta aaatagttgc
    11101 agttgaatca tctgatgaag ccataaataa cgcagatgtg atagttttag ctactccatc
    11161 aaaacaacca gtacttaatg gaaacttagt gaaaaaagga gctttaataa gtgcagtagg
    11221 ttcatatatg ccagatatgc aagagttgtg cccaaactgt ttaactagag cgtctaagat
    11281 attttttgaa tcaacagatg cagttttatc tgagtcagga gatatattaa ttccattaaa
    11341 agaaggtaag ataagtaaag atgattttag tggtgattta gggaatgtta taaacggaac
    11401 tattcctgga agagaagatg atgatgagat aattgtattt aaaactgttg ggataggtgt
    11461 acaagatgtc gtaacagcaa aaagaatata cgataaagca aaagaaaacg gtattggaat
    11521 agactggaag tggtaaaata ttcaaaataa ttctatagtt agacaaattt atgttataat
    11581 gttgataaat aaatttttta ttattttaat tgagggagga tgtttgtatg aaaatggtaa
    11641 aaaagttaat attatctacg attatggctc tagttttagt tctaactata gggtgttcta
    11701 atgcagaaga aaaagtaaag tcgaattttg aagctaattt agctgcatta gaaatgtata
    11761 agtatgatga aaaaggaatt tctgatgcat taacagaagc tgaatataaa cagattcaaa
    11821 cttataacag caaggacaag gtttcaatga ttgctcaatt gacacctctt tatggtaaag
    11881 aaaatgctga aaagatatac gaaacaattg ttaaattaat tcaaggtgtt gaaaagacag
    11941 taacagtgga atcttctagt aaggataaga taactttgaa agttgtaagt aaagctttag
    12001 atactagtaa agtaatgagt gaagcgaaca ctttagtaaa atcttatgta actgatgaaa
    12061 agaattacga taaaaaagaa aaagaagttc aagatgatat gacaaaaatg attatagacc
    12121 tttacaataa gaaaccaact aaggaagtta caactatgat tacatatact aaaaaagatg
    12181 gtaaatggca accacctagt aatatacaaa agttatgtga aggaatatat acaatttaat
    12241 ttattttaaa gacacaaaac tatcttttaa gcatataata aaatgtttgg aagatagttt
    12301 attttatttt caaagtataa attatat
//
LOCUS       DBGJFU010000112        11973 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.112, whole genome shotgun sequence.
ACCESSION   DBGJFU010000112 DBGJFU010000000
VERSION     DBGJFU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.112"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            130..1521
                     /locus_tag="ACOIAS_002924"
     CDS             130..1521
                     /locus_tag="ACOIAS_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HGZ2702618.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1774..3567
                     /locus_tag="ACOIAS_002925"
     CDS             1774..3567
                     /locus_tag="ACOIAS_002925"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HGZ2702619.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3648..5030)
                     /locus_tag="ACOIAS_002926"
     CDS             complement(3648..5030)
                     /locus_tag="ACOIAS_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HGZ2702620.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5288..5635
                     /locus_tag="ACOIAS_002927"
     CDS             5288..5635
                     /locus_tag="ACOIAS_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGZ2702621.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5653..6687
                     /locus_tag="ACOIAS_002928"
     CDS             5653..6687
                     /locus_tag="ACOIAS_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HGZ2702622.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6736..7785)
                     /locus_tag="ACOIAS_002929"
     CDS             complement(6736..7785)
                     /locus_tag="ACOIAS_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HGZ2702623.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8060..9595
                     /locus_tag="ACOIAS_002930"
     CDS             8060..9595
                     /locus_tag="ACOIAS_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702624.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9780..10466)
                     /locus_tag="ACOIAS_002931"
     CDS             complement(9780..10466)
                     /locus_tag="ACOIAS_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HGZ2702625.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10591..11973
                     /locus_tag="ACOIAS_002932"
     CDS             10591..11973
                     /locus_tag="ACOIAS_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2702626.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         4552 a         1469 c         2009 g         3943 t
ORIGIN      
        1 aaaaaatgaa aatacaggcg ataatctatt gagtaaacat agaaaagatg atattataag
       61 tatgtgctaa aatggttaaa gcaatattaa tattaaattt aactataaaa caaaaggggg
      121 cgttttttta tgtcaagtac agaaagcgta tctaaaaaaa gtggtcatcc gaagagtttt
      181 tggcttattt gcatgacaat tttgtgggaa agatttagtt attatggttt aacagcttta
      241 ttggtattat tttttacagc aagtgtagct caaggtggtt taggtctttc tactcaagaa
      301 gcaacatcca tgtttggctt atttacaggt cttatatatc ttacaccatt agtcggtggc
      361 tggttagcag ataaagtttt aggtcaacaa aaatgtattt ctataggtgc acttttaata
      421 gcaataggag atttcgtact atttgcttca tctacatcta ttgcaatggt ttgggtaggt
      481 ctatcaataa ttattttagg aaatggtttt tttaaatcaa gttgctcaaa tatagttggg
      541 gatatatatc ctaaaactga tgtgtcaaga aaagatgcag catacagttt attctatatg
      601 gcagtaaatg taggagcatt tatagctcca ataatatgtg gattgctttc tgataatgta
      661 tttgcagtaa gaggagcaag tggagatata gtatcttatg gatataagat ggcattttta
      721 atttgtggta taggcatgat gttaggtatg attatattta cacttcttgc accaaaatta
      781 ttaaatgaag tcggaaaata tcctgtgact aaaggtaata aaggtgaaaa aattgagtat
      841 ggaccactta ctaagttgga gaaaaatagg ataatagcaa tggttatttt atttttattt
      901 gtggtcttat tctggacagc atactatcaa acacaaagtt cttttatgat ttatgtaagg
      961 gataatgtaa atagaactct atttggattt gaaatgccag tagcatggct tacatcacta
     1021 aatgctatat tatgtgtggc tttatgccca gtattagcta ccctatgggt taaattatct
     1081 cataccaaaa gaggtgattt ctctattcca gttaaaatgg gattaggaat gataataatg
     1141 ggtgttgggt ttatagttat ggtatttgct acattagcta atggtccaga tggaatggta
     1201 aaggctagta tattgtttat agtattgact tatatattta caacagtagg tgaattattc
     1261 ttatctccta taggacttgc aatgttcaat aaattagctc cagctaagta tgctacgttg
     1321 tctatgggag cctggtatct aacaagtttt tttgcaagtt tattatcagg aaagattgct
     1381 ggttttacag ccacacttgg ttttttacag atatttggag gaatagcaac agttttaata
     1441 atatttggtt taatattatt gggaataaga aatgtattaa caagattaat ggctatggat
     1501 ttactaacaa aagaaaaata attcatgacg aattattgta aataaatgaa ttactttaaa
     1561 taaaggaagt tacatttaaa tataaaatta aatgtaattt cctctttgat tatgaaaaaa
     1621 agctttacac aaatagatat tataattaaa attataattg tgataattag agtgtataag
     1681 atttatataa attatacaaa aaatatttat gtaataaact ttacatactt atatatttag
     1741 ttattcaaac tattatttcg aggaggtctt gttgtgaata taaaagatag attaagtgga
     1801 ttaagaaaat ttatggaaga aaaaaatata gatgcctata tgattccatc ttctgataat
     1861 caccaaagtg aatatgtagg agattacttc aaatcaagag aatttatatc agggtttaat
     1921 ggttcagcag ggacggttat tgtgactaaa gacgaagctg ggttatggac agatggaaga
     1981 tattttatac aggcagaatc tcaattagaa ggaagtacaa taaaattatt caaaatggga
     2041 caagaaggtt gcccaactac agatgaatat ttatataaaa atattcctga aggaggaact
     2101 ttaggatttg atggtagagt tatatctgct agagaaggag ctacattagc tgaaaaatta
     2161 tctaaaaaag gtataaagat agaatatcag tatgatttaa tagatagtat atggccagat
     2221 agacctgctt tatcagatag taaagcattt ttattagatg ttaaatattg tggagaaagt
     2281 tttagctcaa aattagctag attaagagaa aaaatgagtg aaaaaggtac ttctactcat
     2341 gtaataacta ctcttgatga tatagcatgg ttattcaata taagaggtgg agatgttaaa
     2401 tataatccag ttgtactttc ttatgcagta ataactttaa aagaagttta tctatttgtt
     2461 gatgaaagta agttaaatga agaaatacta aatgaactag ctaaggaaaa tgttcaaata
     2521 aagccatata atgatgtgta tgaatttgta aaaaatatag acaaaactga aaaagtttta
     2581 ttagatggaa ctaagcttag ttatacaatt tacaataata taccttgtga agttgagaaa
     2641 gttgatgagt tcaatccagt aatgtttttt aaagctcaaa aaaatgaggt agaattagaa
     2701 aatataagaa attcacatgt aaaagatggt gtagctttca ctaaatttat gtactggtta
     2761 aagaaaaatg taggtaagat ggaaatcact gaaataagtg ctactcaaaa attagaggac
     2821 ttaagaagag agcaagaagg tttctttgag ccaagtttca atacaattgc tgcatataaa
     2881 gagcatgctg caatgatgca ctatagtgca actccagagt caaattataa attagaggca
     2941 gaaggattat ttttagtaga ttcaggtgga caatattatg atggaactac tgatataact
     3001 aggactacag ttttaggacc tataagtgat gaattaaaac tacactttac ttctgtagca
     3061 agaggaatga taaatctttc taaggctaag ttcttgcatg gttgtagagg atataactta
     3121 gacatactat ctagaagttg catgtggaat atgggaattg attatcaatg tggaactgga
     3181 catggtatag gatttgtgct aaatgttcac gaagctccaa atggatttag atggagagta
     3241 gtacctgaaa gatttgatag tgcagttctt gaagaaggaa tggtaactac taatgaacca
     3301 ggtatatata tagaaggttc tcatggtata agaacagaga atgaaattgt agttagaaaa
     3361 gctgaaaaga atttctatgg tcaatttatg gaatttgaag ttgttacgtt agctcctatt
     3421 gacttagatg gtatagtacc agaattaatg aataaggatg aaaaagatta tttaaactgg
     3481 tatcataagt tagtttatga taaaatatca cctttcttaa ctgatgaaga aagagaatgg
     3541 ttaaaagttt acactagagc tatatagttt gtactagata caataaaaat cgaggtatta
     3601 taatgcctcg attttttatt gtatatttta ttaactttaa aaaattttta atttgataat
     3661 tctactaatt ttttattcat tataagtagt ataattccaa atatcaataa aaatgatggt
     3721 atcactatga agacttgtaa cataccaagt tggtctataa tagcttgcgt atagccagat
     3781 aatttatttg ctgcaaattt gcctaaaaac caaacaccca ttaggaatga aatatacttt
     3841 tcaggcgaaa gtttatttac cataccatac ccgactggag aaaagcaaat ttctcctata
     3901 ctttggaatg ttaaaaatcc aactacccaa aatacacttg ctttgtgaat tgaattaggg
     3961 tctgttcctg tttgtataac tgctactatc ataaacaaaa atgctatggc taatagtata
     4021 aaacctaagc tcattttttt tggcacactt aaatcgcctt ttttagtttg agataatttt
     4081 acccatacta aagcagagat aggtccaaga ataacacaaa ggagaccatt gtatgaatct
     4141 atccatgaag taggaatttg aaaattgcca acagtaagat ttatgtgttc ctttgtatag
     4201 agtgcaatag acatagaggc ttggttatat gctatccaaa aaaatactgt gaaaaaagct
     4261 aacaccaata ttactattat tctttttttc tcaatcaaag ttaaaggtct tgaagctagc
     4321 attttcttat attctagtga ttttcttata gactctctcc cgttactagg gtcattattc
     4381 aaaggtttat aaaactttct gtttccaaat ataaagaaga ataaacctat tagaaccgta
     4441 attgatgaaa ataaaaatac atatctatat ccatacatac ttatagataa tccgcttttc
     4501 atagcaaacc attcattagc aactaaaccg catattaaag ggccaaataa ggtacctaga
     4561 ttagtagcca tatacgtaat agagaaagct ccatcttttc ttgggtcatc attggtatat
     4621 aagtttccaa ccatagtatg taagtttcct ttataaaaac ctattcctaa tccagataaa
     4681 aatatcatga tgtttatatc catcttacta tttgctatgt atcctagaaa ataaccaatt
     4741 gaaaccatta gcattccaag tataatacag tttttgggac ctaaaaatct atcactgacc
     4801 catccaccaa taactggaga gaaataagtc catgctaaaa aagatgcaag cattgatgcg
     4861 gattctgctg gtgttaaccc taaaccacct ttggagactt gttcagctaa aaacaataat
     4921 aataccaatt tcataccata gtatgcaagt cctacaaagg taaatattga acagcagaaa
     4981 tataaatctc taggttgttt aagtttttct tgcgttttta aaactgacat atgtcgagaa
     5041 cctccagata aatagtttaa caaaatttta ccatactata tgagtatatg tttttttcat
     5101 atagacgtca acttgtaaga taatttaatt aagtcgaaat atatgtaaaa atagtatcat
     5161 ttttgtgtat tcagtaatat ttttttatga taatatatgt tattattata ttaaaataca
     5221 aacatattta tggaaaggat atgccaattt agtagttaga ttgattgttt ggcaggtggg
     5281 atgagctatg ataagaaaga aatctgaatt acaagtagaa gtaaaagaaa atttaaatgg
     5341 tggtatagga aaggttaagc ttgaaaacat aatacaaaat gaagagctta aaggaaaagg
     5401 aagactattt aaaagagtag ctttaccagt aggaagttca attggtgtgc atgaccatac
     5461 aacagatttt gaagtatatt atatactaaa gggaaaaggt aaagtatttg ataatgggga
     5521 atttgtagaa gtaaatgagg gtgatgtagt gtatacagct gatggcgaaa aacattctat
     5581 agaaaatata ggagaagaag atcttgagtt tgtagcacta gttttatata tgtaaaatat
     5641 ggaggaggag tcatgacagg caagatagaa gaaattaagg acaagataaa tagattttgc
     5701 aactccaatt taaatcaaga atacaaagat ataagcttta aaattttgca aaacctttta
     5761 gataataata aagagataat tcattcttct agagctgata tatggtcttc agctatacta
     5821 aatatagttt tagaacaaaa tcttttatac aataagaagc atccactaca tataacaaaa
     5881 aaagagtttt caaaacaaat aggtgtttct ttaaatacta taaataataa atcgtcttta
     5941 ataaaagatg tttgtagtat tgacttttta aatcaagata ctatagctat ttctaatgta
     6001 gaattttggg taaaagagac caattccaaa tcaaagtctg tagatttaga tttagaaaag
     6061 aaaaaaatac tatataaaag atatattaag ctatcacaag atgcgaatag tgatattgaa
     6121 tcaattagat atatggagga agctgtaaat ataggtaaaa gtatgattac taaagaagat
     6181 aaagaacttg gattttggaa aggtttatct accagagcct atatgatagc acttgaatcc
     6241 cttgctagaa agttagaaag tattaataat ctaaaggagt ccaaaaaagt attagaatat
     6301 ttaatagaaa ttaatcctag tgatgagcag ggaataagat acaaattatt aaatgtatta
     6361 attagattaa atgatagaac ttcaataaat aaattatttg aactttataa agaagaaaaa
     6421 tctgctacat ggatgtacag taaagctctt tattacttta aaaataaaag tatgtttttg
     6481 gcaaatgatt ctattaaggt tgctagaagt aaaaataagt acgttggatt atatttgtta
     6541 gattggcgaa atgcttttgg tagagaattt gttacagagg aagaaaaggc agaagctgtc
     6601 tattactatg atgaaaacat agtgatttgg aatgaagtta aaggttctat ggattggcta
     6661 ttaaaaaaaa tgttggaatt ttcataaaaa aaactactcc ttacttttat ataagtaaaa
     6721 ggagtagttt ttaatttata catctagttt taaatcttca ggttttatta gacctttttt
     6781 tctcataaat tctgaaatca acaaagttac aatagcagga agtataaaat taagtaaaag
     6841 tataccaacc cacatagata tacctccttt tccagcactt tgcatagcag ttactgttcc
     6901 aaattgtcca accaatccac aagtacccat accagaacca ataggaatat tctccatttt
     6961 gaatattgtt gtagataatg gtccaagtaa tattgaagct acagtaggtg gtatccaaat
     7021 tttccaattt tttattatat taggtacttg caacatagat gtcccaagac cttgtgccac
     7081 aaggccgcca actccatttt ctctaaagct cataacagca aagcctacca tttggcaaca
     7141 acaacctaca gttgctgcac caccagcaag accacctaaa cttaacatca tacaaatagc
     7201 tgcactactt ataggtaatg taagagctat tccaacaagt gcagagacta aagctcccat
     7261 tattataggt tgcatagttg tagcttgcat tattatattt ccaaatgaag tcataaaagc
     7321 agaaacagct ggaccaataa gttcagctac aagtactcca attagtattg ttacagcagg
     7381 tgtaaccaat atatctattt ttgtttcttt tgaaactaat tttccaacct ctacagcaaa
     7441 aagtgtagcg acaaatgctc caacaggtcc tcctaattca ttaccacaag ctccaacaag
     7501 tgcacaagaa aatagtacaa gtggaggtgc ttgaagtcca tacgcaatag ccactgctat
     7561 agctggacca gtaacctgtt tagcaagagg gcttataact tcagtaaaaa gagaaatatt
     7621 gaaattatct cctaatgtat tgaaaattgt tccaattaaa agggacgaaa aaagaccgag
     7681 tgccatataa cttagtgcgt caataagata cctttgaaca gaaatttcaa tattttttct
     7741 tttagcaaat gatttgaaat ttcttttttt ataattgatt tccaatttaa aatcctcctc
     7801 atataatata agtataattt ctttttaatt aattatataa cttttcagct gacaagacaa
     7861 atgttatttc actaaaatta gactattgta atagtaagaa attaacatta attttagttt
     7921 taaattttaa atttatggtt tataattagt atagggatat tctgaaatat aataattttc
     7981 cagtttatcc caatatttaa cttaggtaga ttgcttacaa aacaaatgat tatatattag
     8041 gctaaaagga agtggataga tgatatttgt aaaaaataag aaatacagaa aaattgccat
     8101 aattatatct attcctatat tgcttgttgc gcagtactac ctttttaaat ttggaatact
     8161 tagaccaatg gtaaagggag tagaaattga aattgtagat ggggaatatg ttaaagatat
     8221 agataaatat gtagtaaaat taaatgaaac agttactttg tctacaggtg aatatattaa
     8281 ggttccttcg tatgctaaaa atccaaatat ttggtttaat gtattggata actcagggac
     8341 tttaaaaata gatggaaata aaatgacagc attaaaggtt gggtattctt ctgtgggtat
     8401 catgaaaaat tcaagagtat taaaaaaggc tactataaaa gtagtagacc cagaggtaga
     8461 atcactagat atggagatta atggggattt aaaacatgtt ggtgatagtg ctactataga
     8521 aagtacaata gaagttgatt atgataagtt taaaaaatca tataagccag tttataagag
     8581 tagcaatgaa aatattttaa aaatcaaagg aaataaagta agtgcagttg gagttggtaa
     8641 agctacaata tatgctaagt gtggaaataa agaaattgag agaacattta ggattcaagc
     8701 tagagttgct gaaataaata tggatgatat aaaaatagaa gttgaccaga atgaaaaatt
     8761 gaaaccagat ataataactt ctccacctgg tttagaacca ccaaaaatat attatgagtt
     8821 agtagattca aaattacctg tagaaagagc aataagttta agtagtaatg gaaatgttgt
     8881 agggcttaga gaaggtaaag atagggtgag aataacttgt ggagataaat ctaaagtaat
     8941 aacagtaaca gtagtcaaag aaactataac gaatgactat atacaaaact tggttggttc
     9001 acataaaata gtaaacaata aagttattat taaacttgaa tgggattata tgaaagatgt
     9061 ctttgattat gatatatatc ttaaagataa gttgtcagga gaatcattta agaaaattaa
     9121 atctataact ataaatgaaa gtgatttagg taagtctaac aaggtatatg caactataga
     9181 agtagatatg aagggtaatc aaaatataga ttttgatatt tatgtcatag gtaaaaagga
     9241 ttcagataca actaagccaa gtaatgtaat aaatattacc aatggaaatg gaaattcaaa
     9301 taatgataat aatattaaag atatgagggt agaaaatata tcagctaatg ttgatagaga
     9361 taataatgtc attagagtta actggagtag tatatctcat aaggactgta cttacagtat
     9421 atatgtaaaa aataatacta atggagatgg agaatttact ctatatcaaa gtgatataca
     9481 gggaaatgag catactataa gtattcctga aggagattta aatctagata tttatgttgt
     9541 tgcaaattat aatggaaata gctctcaaaa tagtgatgtt ataaatgtaa gatagatttg
     9601 tatacaaatt gaaagtgttg agtttattta aatattaaat taagaaaaag gatagtatag
     9661 ggtaattata ctatcctttt ttgttacaat atataggttt aaaatctcta gtaatataaa
     9721 ttataaaaag tcttatttac acaaatctat agatattttt tttattaatc aatattgtct
     9781 taaattgaac ttagtgcggc ttctattgca tgtattactg tcgtatcaaa tagtggaata
     9841 gaagtgtcat tttgtttaat taaaagacct atttctgtac atcctaatat aacagcctct
     9901 gcaccttgtt tgccaagctt atctataata gataagaaag ctttttttga agattcagat
     9961 ataattccta agcaaagttc attaaatatt gtgtcattta ctattatacg gtcttcttca
    10021 tttggaatta agacttcaat tccattatta ataattacat ttttataaaa atcttgttcc
    10081 attgtatatt tagtacctaa taaggccact ttttttattt ctttttccct taataccgta
    10141 gcagttacat ctgctatgtg aagtaatgga atatgtatac tttcttgtat tttatctgat
    10201 accttatgca tagtattagt acagataaca ataaaatctg cccctgcctc ttgtaattta
    10261 attgcagcat cagctaaaat tttggcgctt ttttcccaat ttccactaga ttggcattct
    10321 tcaatttctt gaaaatctac actgtaaagt atacatttag aagagtgtag tccacctaat
    10381 ctttctttta ttactgtatt aataacttga taatatgtaa tggtactttc ccaactcata
    10441 ccaccaatta agcctatagt cttcatatta tacctctctt atttatttat tttgaaatag
    10501 ctatttacaa atacaagtat agcatctaaa atagaagcat acaataataa aattgtatgg
    10561 agtacaatta taattaagag gagtaattat atgccaataa attcttttga aaactatcct
    10621 atgacatgga aaccaaagtt agataaccga aaaccaccaa tatataaaac tttagcaatg
    10681 ttattagaag aagatattaa aagaggaaat ttaaatcctg gagataaatt gcctccacaa
    10741 cgggaattag cagattttct agatttaaac ttaagtacaa taacacgagc atttaaactt
    10801 tgcgaagaaa agggactaat atgtgcaaaa gtgggaaagg gaacattcat ttcttctgat
    10861 gtaaatgtaa gtaatacatt attatatcaa acggaatcaa aagacataat tgagttgggg
    10921 acagttcatc ctccgtatga gcaaaataca tatattattg actttattaa aaatgtacta
    10981 aaacaaccag aaatggatag gtttttacaa tacatgtctc catctggcac atatatgcaa
    11041 aaaaagtcaa ttgcaaaatg gatagaaaga aataatgtat acaccagtga agaaaatata
    11101 ttattatcta caggagggca aaatgctata tgtgcaactt tactaggatt atttaaagct
    11161 ggtgatagaa ttgctacaga ttctttaagt ttttctggaa ttaagtcaat agctaaaatg
    11221 ataggtattc aattagtccc aattcctcaa gaaaataatg aaatatcgat tgaatatctt
    11281 gaaagttatt gtaaaaatga aaatataaaa ggcatatatc ttataccaga ttatcataat
    11341 ccaacgacac atactatgtc tgatttatct cgtaaaaaga tagccaagat agcaaagcaa
    11401 tataatataa taattattga agatgctata aatagcattt ttagagatgg tatacaaact
    11461 cccatatttt ctttagctag tgataatact atatatattt ttagtacatc taaattttta
    11521 tgtgctggtc ttagagtagc atttgtagtt gctccaaaac gatatataga aaatttagaa
    11581 aatgcactat ataatatgaa tctaatggtt tctccattta cagcagaaat agtacaccgt
    11641 ttattatatt ctcctattat agataaaatt atagaagaga aaagagatgc aattatagaa
    11701 agaaatgaag ttgctgataa agttttgtca gattataatt tgattggtga taagaattgc
    11761 agttttcgtt ggttacttct tcctgatgaa ttggatggaa aatcatttga aatttctgca
    11821 aaaaatctag gagttcaagt atattgtgcc gaacgatttt cagtaggaaa ctctacagtt
    11881 ccaaaagcag ttcgtatttg tgtaacagct ccaaaagatg tagaagaatt agaaaaaggg
    11941 cttaatataa taaagtcact tcttaagagt taa
//
LOCUS       DBGJFU010000113        11893 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.113, whole genome shotgun sequence.
ACCESSION   DBGJFU010000113 DBGJFU010000000
VERSION     DBGJFU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.113"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            251..1291
                     /locus_tag="ACOIAS_002933"
     CDS             251..1291
                     /locus_tag="ACOIAS_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2702627.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1647..2969
                     /locus_tag="ACOIAS_002934"
     CDS             1647..2969
                     /locus_tag="ACOIAS_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGZ2702628.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSIITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3266..4627
                     /locus_tag="ACOIAS_002935"
     CDS             3266..4627
                     /locus_tag="ACOIAS_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGZ2702629.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTAIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4700..6094
                     /locus_tag="ACOIAS_002936"
     CDS             4700..6094
                     /locus_tag="ACOIAS_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2702630.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6156..6626
                     /locus_tag="ACOIAS_002937"
     CDS             6156..6626
                     /locus_tag="ACOIAS_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HGZ2702631.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6629..8845
                     /locus_tag="ACOIAS_002938"
     CDS             6629..8845
                     /locus_tag="ACOIAS_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HGZ2702632.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8928..9599
                     /locus_tag="ACOIAS_002939"
     CDS             8928..9599
                     /locus_tag="ACOIAS_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2702633.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9962..11176
                     /gene="dpaL"
                     /locus_tag="ACOIAS_002940"
     CDS             9962..11176
                     /gene="dpaL"
                     /locus_tag="ACOIAS_002940"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGZ2702634.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11303..>11893
                     /locus_tag="ACOIAS_002941"
     CDS             11303..>11893
                     /locus_tag="ACOIAS_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2702635.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4500 a         1368 c         2404 g         3621 t
ORIGIN      
        1 gaacttaaat taaaaatttt aaaaattatt tccatataaa atatggcatt gtaattgctt
       61 atttaataga gcatttttgt aaaatgaaga aagtatatct atcaaattaa aactaagtag
      121 atatactttt tattaagttt tgttttaaaa taatattatt aaattataac taagcttttc
      181 tgttatgata taattaaata gataatgtga atgattatta tatagaatac ttatttggag
      241 ttgatatttt atggacaaac acagtgattc agtaatgtat ggtggaatgg aagtatataa
      301 tatttgggga acaacaacag tagactccat aattggagag tctgatgtaa tgcgtgctct
      361 taagaataga atttgtaaaa ttggaaatag tgattctact gtagctataa ctggagaaag
      421 tggcactgga aaagaattaa tagcaagagc cattcatctc gcaggaaaca gagggaataa
      481 aacattcgtt gctataaact gtgctgctat accagaacct ttactagaaa gtgaactttt
      541 tggatataca aaaggagctt ttagtggagc agattcaaaa ggtaaaattg gtaagtttga
      601 attggccaat ggaggggttg tatttttaga tgagatagga gatatgccaa ttcaactaca
      661 atcaaagtta ttgagggtat tacaggaaaa gaagtttacg agaataggtt caaatgaact
      721 aattgatata gatgttagaa tcatatcagc tactaataga gatttatttg agttggttaa
      781 ggaaaataaa tttagagaag acttatacta tagattgaat gtaatacctt tagaagtgcc
      841 tccacttaga gaaagaggag acgatataca aatattaata aacaatttta tagacaaata
      901 ttcgagaaag atgaagaaaa acgtgtacca tatagagcca gatgtggaac aaatgttgct
      961 taagtatcct tggccaggaa atattagaga gttggaaaat actatagaac tggcattaaa
     1021 ccttcttgag gaagatggaa taatacataa aggaataata aatagaaaga tacttgaata
     1081 ttttaagtta aataatataa atataaagtt acttgaggaa agtgactatg agataagtat
     1141 ggaacaagac atacttacac ttgaagagtt tgaaagagcc tatataaaga aagtgttaaa
     1201 gttttacgga aatgatacta agtctaaaaa actggctgcc aaaaaacttg gaatatcact
     1261 agctacattg tacagaaaaa tagatgtata ataattaggc tttttattct caattctaaa
     1321 atttgaaatt ataaaattga taaaaaacac ttggaaaact attttctaag tgtttttttg
     1381 tgtaatagca ttttattatc caaaaataaa aaaaatttaa aagaatttaa aaaaataaat
     1441 gctttaataa attatcaaat actaacaaat ataataaaaa ttatatatgt aaaacaatta
     1501 tgttaaaaaa ttggcattat aattgcttca tattaagttg agatgacaaa ataaaaaaag
     1561 aaaggatgtt gtgtgagata ataataactg tcattaaaaa atagttggga gtgataacct
     1621 tgagtaaata tcaaaagaat gtttctatgt ttgatattga tggaaggcca ccaattttaa
     1681 agtgtttgcc actatcaatg caacatatac tagcaatgat agttggaact gttacagtac
     1741 ctatagtcgt aggaggagct gtgggtgtta gccctacaca aataacactt ttagtgcaat
     1801 atgcattaat aatagctggt atatcaacac ttatacaact atatccaata ggaaatattg
     1861 gttcaagatt gcctataatt tttggagttg gttttactta tgtaccaaca cttgtagcag
     1921 tcggaggaaa ttatggatta gcaagcattt tgggtgcaca attaataggg ggagtggcta
     1981 cagtattaat agggatattc ataaaaaaga taagaaaatt ttttccgaat atagttgcag
     2041 gaacagtggt ttttacaatt ggattatctt tgtatcctat agcaataaat tatatggcag
     2101 gtggtgtaaa tagtcctact tatgcctcta tgcaaaactg ggcagtagcg gctataacat
     2161 tagtggtagt tataggtctt agtcaatttg caaaaggata tgcaaaattg gcagcaatat
     2221 ttttgggaat tatagttgga tattgtgtat ctttagttct gggtataata aattttgacc
     2281 ctattagaga ggcaacttgg tttgcgatac ctaaaccgct tgaatttggg atggaatttg
     2341 atatgcctgt aataatctca gtagtaattg taagtataat caatgctgtt caagcaattg
     2401 gagacttatc agctactaca atgggaggca tggataggaa tatcacagat aaggaattat
     2461 caagtggagt aataggaagt ggaatttgta ctatgatagg agctttgttt ggaggtttac
     2521 caccatcatc atatagccaa aacgtgggga tggtagctat gaacaaagta ataagtagat
     2581 ttgtgcttgg aatagctggt atatttatgt tattgtcggg gtttgtgcct aaatttggtg
     2641 cattaatgac tactatacca tattctgttt taggaggagc tacaatatct atatttagta
     2701 taataacaat gacaggtata aagttaatta ttacagaaga gttgtcatca agaaatatta
     2761 ccattgttgg tttggcaatt gccttaggca tgggaataac atcagtccca gcagcccaag
     2821 aggcatttcc ttcatttgta aagttagtat ttggagaatc tccaatagta attgcaacat
     2881 tggttgcatt cgttttaaat ctaataatac caaataaaag tctagaggat gaggctaaag
     2941 aaagagctgt tatggaagag actaattaag atataataat ttaaaagaat tgtttaaatt
     3001 atttagtatg agattgttag attatttttg atagctattc attttaataa aaaatttaac
     3061 aaataaatat ataccaggca attaatgttc atttaagttg aaaatcgggg aggcaatatt
     3121 tagaaatttg ttaatttttt ataaaaatag ctcagtttag ctgtgtttat cggttgagca
     3181 ataaattcat ggtcataaaa atatttatag aaaataatta taaaaatatt tagtcaagat
     3241 atttatgtat gggaggattt attttatgaa aacacgtaat acttcaaaat atgatgtcga
     3301 tgggatacca ccactaaagg aatcaatacc tttggcctta caacatctgt tagcaatgat
     3361 agtaggaaat atggtacctg cgatattaat agcaaacgtg gtaggactta accaggggca
     3421 agcaacaatg ttgattcagg gttctatgtt agcagcaggt ttagcaacgt ttttacagtt
     3481 atatccaata cctttattta aaggatttaa gttaggttca agattacctg taatgatggg
     3541 aatgagttat gtatttttag gagcttgtct ttcagtagca gctaaagatg gtttagcagc
     3601 tctatttgga gcacagattg ctgctggggt aatcgtattc tttgtaggat ttggagttaa
     3661 gaaaattaga catatattca ctccaatagt ttctggaact ataatagcat gtatgggact
     3721 tggactattc gcaacagcta taaagaattt agctggagga gaaggcacac aaacatttgg
     3781 aagcccaata aacttcacag taggagttat agttgctttt gtaataataa tgataaataa
     3841 atacggtaaa ggtttagtaa aaaattcttc aatattaata ggaatattag taggttatgc
     3901 tatatcttta gttttaggat tggttgattt ctcagccgtt caaggagcag ctatagtatc
     3961 tttaccaaca ccagcagctt ttggattaga atttagacca gagttaatag taatgtttac
     4021 tattatttac ataataggta tagctgatat gatgggtgcc tgtacaatag ctacagtagg
     4081 agcaatggat agagaggtaa ctgacgaaga attagcatca gttgtattag gaaattctat
     4141 aacttctata atttcatcac tatttgcagc tcttccaact ggagtattta gccaaaatac
     4201 agcaattgta tctatgaaca aagttacaag tagatttgta atagcactag gggcacttgt
     4261 attattacta gcagggatat ctccagcatt gggtgcaata atgacaacta taccatcttg
     4321 tgttgttggt ggagcaactt tagtagtttt ctcatcaata gcaatgtctg gtttctctat
     4381 tatgagtatg gatggattca ctgaagaaaa caatcttata gcaggtgtat ctatagcaac
     4441 aagtatgggt cttacaactg ctccacaagt tttagaccaa ttcccagaaa caattggaac
     4501 tgtccttgga gggtcaagta tagtttctgg agcaataata gctatattac ttcaaacatt
     4561 atttaaatta aagtcacgta aaagtgctga aaacgttact tcaaatcttg aagaaaatat
     4621 tggataaatg tattagatat aatatgagaa aaccatatgg taaaatgttg taatgaaagt
     4681 attcaaaggg ggatttaaga tgattgatat actaataaaa aatgcaataa ttgttacagt
     4741 taataaagac agagatgtta tatttgatgg agcaatagct attcaaggtg acagaatctt
     4801 ggacatagga aatacagttg atatcgagcc taagtatctt gatgctaaaa aagttataga
     4861 tgctaatgga aaagtaatat tccctggctt tataaatact cataatcacc tgttccaagt
     4921 gttattaaaa ggtttaggtg atgatatggc acttcatgag tggttaaata caatgatgtt
     4981 tccatcagca aaattcttaa cagaacaaga tacttatgat gctgcaatgc ttggatgtat
     5041 ggaaggttta aaaagtggta taactacaat ggttgattat atgcatactc acaatagacc
     5101 aggacttaca gatggaatag taaaggcata taaagattta ggcataagag gggttgtagg
     5161 tagaggttgt atagatttag gtattcacaa agagcttata gaagatgtgg aaacggttga
     5221 aaaagatttg agaagggtat tcgaaaaata tcataattca gaaaatggaa gaataaaaat
     5281 ctgtgtagct ccttcttcaa tgtgggctat atctgaagaa atgggtaaga tgctttggaa
     5341 tatagttaaa gaatatgatt catattttac agttcatata tctgaaacag aatttgcaag
     5401 aacagcaaca aaggatatac atggagaaat agatataaaa ttacttgaaa aatggggtat
     5461 agtaggtcca gaagttgtag cagtacattg tgtatgtata actgatgaag acatggaaat
     5521 gcttaaaaag tatgacataa aagtttctca taatgtagca agtaatatgt atcttgcttc
     5581 tggtgttgca cctgtgccag aaatgttaaa aaaaggtata aatgtgagtt taggtcttga
     5641 cggtgcagca agtaataacg cacaagatat ggtagagctt atgaaactaa cagctttaca
     5701 acacaaagta aataattgtg accctctggc aatttctgca gagaaagttc ttgaaatggc
     5761 tacaattgag ggagcaagaa ctcttagaat ggaagatgaa ataggttctt tggagattgg
     5821 gaaaaaagct gacctagtaa tatttaactc aatgctttct ccaaaatcaa tacctcttca
     5881 caatcctgta tcaactttgg tatactcagc tagtatgcac aatgtagaga gtgttttagt
     5941 tgatggaaat gtaatacttg aaaatggaaa agtaactaca attgaaagtg aagaacaagt
     6001 gttgcttaat gcacaacatg cagcagaaga attatgtaag agagctaatg taactaatag
     6061 aatggaagga cataagtgga atagcttata ctaatattaa aataagattg taataatatt
     6121 ctattacaac attagtttat ctgtaggagg agaaaatgga aaaaataaaa ttaaatatta
     6181 atgattacat gtatgaaata gaagttgaaa agaatgaaac tcttttgcat gtacttcgtg
     6241 aaaaattaaa tctaactgga gcgaaacaag gttgtaatag tggcagttgt ggagcatgta
     6301 aagttttgat agatggagtt gacatgaaat cttgtaaact tcttgcaaaa aactgcgaag
     6361 gtaaagttat atacactata gaaagttttg ctaatggaga aaatcttcat ccaatacaag
     6421 aagcttttat agaagttgga gctgtacaat gtggatattg tactcctggt atgattataa
     6481 caacacaagc tcttttaaat agaaatgcat ctcctaatga agatgaaatt agagaagctt
     6541 ttaaagaaaa tctctgtaga tgcacaggat atgtaaagat tatagaagct gtaaaattat
     6601 ctgcagctag attagggggt agataattat ggaaaaggta ataggaaaat cataccctct
     6661 aaaagatgca gctttaaaag ttactggtca aatgaaatac gtggctgata tgaaaccaca
     6721 aaatgtactt tacgctaaga tgcttttaag ccctgtagca catgcaaaaa taaaaagtat
     6781 agatacaagt gaagctgaga aagtagtagg tgttagagct atagctactt atctcaatac
     6841 atcacaaaga aaatacaatg gttcattaag attttatgag cacaaaatac ctgaaaatga
     6901 agttatattt tcagatacag ttagatttgt tggtgaccgt gttgcagcag tagctgcaga
     6961 gactccagaa atagcagaga aagctgtaaa acttataaaa gttgaatatg aagaattgcc
     7021 agctgtattt actatacaag atgcaataaa agaagatgct atctgcatac atggagattc
     7081 aaatatagtt tctcaaaaca acataaatgg gggagatgta gaaaaaggct tcaaggaatg
     7141 tgactatata tttgaagatg aatactctac tccagcaata catcatagtg caatagagac
     7201 tcatagtgta attgcacact atgattataa tgataaatta actgtacata caccttgcca
     7261 aaatacattt ggatttagaa taatactatc tcaaattttt gggatgtcat taaataaagt
     7321 aagagttata agacctgcta ttggaggctc atttggtgga aaattagaac ttacaattga
     7381 accagtagca gccgcattat caaaaatgac aagaagacca gttaaaatgg ttcttacaag
     7441 aaaggaatct atgatatcaa caagaactcg tcatgggtct tttgtaaaat taaaaactgg
     7501 tgtaaataaa gatggtgaag taatagcaca agacataaaa atatatacaa atacaggagc
     7561 atatgcatca agtgctttaa atgttatagg agcacttagt cataaagtat ttaaagttta
     7621 taaaatacca aatataaagt ttactgggat gcctgtatat acaaatacac caatagcagg
     7681 agctatgaga ggctatgggt cacctcaaat atttatggct caacaagctc aatttgcgaa
     7741 aatagcaaag gaaataggta tggatttagt cgattttcaa aataaaaatg cagtagagcc
     7801 agatgatgtc gatataatct tccacggttc tcttggaaat cctagagtat tggattgtat
     7861 agagcaaggt aagaagatgt ttaagtggga tgaaaagaaa aaacttccaa aagaagaagg
     7921 aaaatattta agaggtatag gtatggcaat aggtgcacat ggtaatggag tttttggtgc
     7981 acatagagat gttgttacat tgactcttaa attaaatgaa gatggtacgt tgacactgct
     8041 aacaggagct catgatatgg gtaatggtgt tgtaactatg caaacaatga tggtagctga
     8101 agttttagga ataacacctg ataaagttga tacatttgaa actgatacgg atgcatgtac
     8161 attcaacctt ggtgactatg caagtagagg ggtatttgta gaaggtggag gagctaaaaa
     8221 gacagcagag aaattgaaaa atcttatatt agaagaagca gaaaaattac ttgaaacatc
     8281 aaaagaagat ttatatctag acaatggatg tgtaattagt aaacttgatg aaaatgtaaa
     8341 agcatcactt tctgatgtcg cagtatatag tcaaagtaaa tcattaagag agttaactgt
     8401 tgttgaagat tattcttcac cagctggacg tacttcttat ggtgtacatt ttgctgaagt
     8461 tttagtagat aaagaaacta aggatataaa agtagttgat tttgtagctg tacatgatgt
     8521 tggtagagtt ataaatcctc taaatttaga aggacaatta gaaggtggga ttcatatggg
     8581 tctaggatac gcattgagtg aagaattggc atttgatgaa caaggtagac ctaaagctac
     8641 tgattttaaa acatataaat tatttagaac tgtagatatg ccaaaatgtc aagttgcatt
     8701 tgtagatggt tatgaaaaag ctgggccttt tggtggtaag agtattggag aatgtgccgt
     8761 tgtaccagtt gcgccagctg ttgcaaatgc agtatataat gcaactgatg tagagataca
     8821 ttcattacca ataaaattaa aatagtataa attaaagcag aaataacaat ttattgtatt
     8881 tgtaaataga taataaaaat agatttattt ggtgaggaga tattattatg gcaaagatat
     8941 taggtatttg tggaagtcca cgtaaaggag caacagaata tgcagtgtta gaagctttga
     9001 aagaagcaga aactatacct ggtatagaaa ctgagtattg gtcagttaga gggaaaaaaa
     9061 taagtccttg tgtacattgt gatgcatgta taagaaaaga aactatgtgt attataaaag
     9121 atgatatcca agaattagag caaaaaatat tggaggcaga tggaattata gtaggttctc
     9181 cagtttatga tatgaatata actgctcaac ttacagcagt atttaataga cttcgtccaa
     9241 tgtatttagt tcatccagga aaacttcaaa ataaggttgg tgcagctata actactggtg
     9301 gaactagata tggtggccaa gaattgacaa aacttcctat aataaatttc ttcctaatgc
     9361 atgaaatgtt agtgtctgga ggtttaggtg gatgctatat aggtggtaca atatggagta
     9421 aagatggaaa agcaaaaggt gcccaagagg atgagacagg tatggacaca gtaaaaagac
     9481 ttggaaaagg tgcagcagag gcagttatgg tttctaagtt tggtcttgaa aaatggaatg
     9541 tcgtaaaaga agaattagat gttaaaaaag atgaaaaatc acctcttaga gaccactaaa
     9601 gatttattat attattgaat atatacaaat agtgatgtaa tagattattg catcactatt
     9661 ttatgtttta atttagattt tatagatttt atattaaaaa atatagaaac aaagtgtttt
     9721 gagatttatt tttaaatttt aaaaatgaga attaaaaagt gagaagatag aaaaggtttt
     9781 ttagttatta aattctacat acattgtatg aactaaagat agaaaagaaa aaataataaa
     9841 agcattgcaa tttctatact aaagagtcat agaaaaatat ctagtatata tttagtgttg
     9901 gcatgttaat tgcttatata ttaaagtata aaaatttaac aataatttgg aggtatttta
     9961 gatgttaaaa gaaattaaat ggaaagtaaa taatttgcca aagggcgata aggaaaattg
    10021 tatcaaattt ttaaatgaag aagaaataac taaggttaga aattttcaca aaagctttcc
    10081 tcaatataaa gaaactccat tagcaaacct agaaggttta gcaaaaaaat taggtgttgc
    10141 aggagtatat gtaaaagatg aatcttatag atttggatta aatgcattta aagtattagg
    10201 tggttcttat tctatgggta gatacttagc tcaaagatta gatacagata tatctgagtt
    10261 agggtatgac aaattaactt caaaagaaat aaaagaaaaa ttaggagaaa taactttctt
    10321 tacagctaca gatggaaatc acggtagagg agttgcttgg acagctaata aattaggtca
    10381 aaaatctgtt gtattaatgc caaaaggttc ttctgaattt agattaaata aaattaaagg
    10441 agaaggtgca gatgctagta taacagactt aaactatgat gatgcagtaa gattagctaa
    10501 tgattatgca gaagctgatg accatggagt aatggttcaa gatacagcat gggacggata
    10561 tgaagaaata ccagcgtgga taatgcaagg atatggaact atggctcaag aagctataga
    10621 gcaattaaaa gaatatggag tagatagacc aacacacgta tttgttcaag caggagttgg
    10681 ttcacttgct ggtgcagttc aagggtatgt tgcttcaata tatgatgaat gcccaataac
    10741 agtagtagta gaggctgatg aagctgattg ttactataaa tcagcagaag ctggagatgg
    10801 aaaaccaaga tttgttggag gagatatgcc aactataatg gcagggcttg cttgtggaga
    10861 gccaaatact ataggatttg aagtattaaa aaatcatgca gcagcatttg tttcagcacc
    10921 agactgggtt tctgctaagg gtatgagaac attaggtaat cctttaaatg gtgatgaaaa
    10981 ggtaatttct ggagaatctg gagcagttac aactggtctt ttagtagcag ctatggaaag
    11041 agaagattta gcagatttaa gaaaagattt aaaattagat gaaaattcaa gaatactttt
    11101 aataagcaca gaaggagata ctgaccctga caaatataga agtatagttt gggatggaga
    11161 atatccaagt atataaggaa ttactaaaat gcaattatta gatattggtt tacatattta
    11221 gaagatatat aagttctaaa atttagaaaa tatataagta tataaaatac atacattata
    11281 aaaaatctag ggggataatg aaatgttaaa cgaacaaaga aaacaagaag taactgaaat
    11341 atgccaaaaa ttaattcaaa atcctagttc ttctggaaat gaagaaggtg ttgtaaaagc
    11401 aatagaagag tcttttgaaa aattaggttt tgatagttgg tcaagagata gatatggaaa
    11461 tatagtagga tgtataaaag gtaacaagcc aggtaagaaa atattatttg atggacatat
    11521 agatacagtt ccagttccag atgcaagtaa atggagtgtt cctccttttg aaggaaaaat
    11581 agttgatgga aagatatatg gtagaggaac ttctgacatg aaaggtcaat atactgcaat
    11641 gatgtctgct gtagcgtatt ttgctaaaga cactaataga gattttgctg gagaactata
    11701 tgtagcaggt gttgttcatg aagaaatatt tgaaggtgtt tcggctagag agataagtaa
    11761 ggctgttcaa cctgactatg ttgtaatagg tgagtcttca gagttaaact taaagattgg
    11821 tcagagaggt agaggagaaa tagtagtaga aacttttggt aaaccagctc actctgctaa
    11881 tccagaaaaa ggt
//
LOCUS       DBGJFU010000114        11737 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.114, whole genome shotgun sequence.
ACCESSION   DBGJFU010000114 DBGJFU010000000
VERSION     DBGJFU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.114"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            136..921
                     /locus_tag="ACOIAS_002942"
     CDS             136..921
                     /locus_tag="ACOIAS_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGZ2702636.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDAMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKAYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            1200..3110
                     /gene="phnW"
                     /locus_tag="ACOIAS_002943"
     CDS             1200..3110
                     /gene="phnW"
                     /locus_tag="ACOIAS_002943"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HGZ2702637.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            3469..4290
                     /locus_tag="ACOIAS_002944"
     CDS             3469..4290
                     /locus_tag="ACOIAS_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702638.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            4331..5098
                     /locus_tag="ACOIAS_002945"
     CDS             4331..5098
                     /locus_tag="ACOIAS_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HGZ2702639.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            5106..5726
                     /locus_tag="ACOIAS_002946"
     CDS             5106..5726
                     /locus_tag="ACOIAS_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HGZ2702640.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(6026..6613)
                     /gene="rbr"
                     /locus_tag="ACOIAS_002947"
     CDS             complement(6026..6613)
                     /gene="rbr"
                     /locus_tag="ACOIAS_002947"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HGZ2702641.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6699..7136)
                     /locus_tag="ACOIAS_002948"
     CDS             complement(6699..7136)
                     /locus_tag="ACOIAS_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2702642.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            7240..8238
                     /locus_tag="ACOIAS_002949"
     CDS             7240..8238
                     /locus_tag="ACOIAS_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HGZ2702643.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(8358..9284)
                     /locus_tag="ACOIAS_002950"
     CDS             complement(8358..9284)
                     /locus_tag="ACOIAS_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HGZ2702644.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNTYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            9670..11274
                     /locus_tag="ACOIAS_002951"
     CDS             9670..11274
                     /locus_tag="ACOIAS_002951"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HGZ2702645.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         4632 a         1258 c         1985 g         3862 t
ORIGIN      
        1 ttaatgataa tacaaagtct tttgaaaata actttaatta agtaaaaaat ttaatacata
       61 aatatgattt cataaatctt aggtatcatt gtaatattat aggagaaaaa tatattatat
      121 ttaggaggaa aatgaatgtc tttaaattcg atagatagaa aaaaagaaat acttaaatta
      181 ctagattcaa aagggaaggt aaacactaaa gaattagtaa aaagattaga agtatcatca
      241 gaaacaataa gaagatattt ggaagaatta gaaaatgaaa ataaattaaa aaaggtatat
      301 ggtggtgcta taaaaataaa ttatggagtg attgaaccag aacatataaa gagaaacaat
      361 actaacctag ataaaaagca aagtattgct aaatatgcag ctaagtttgt aaatgacaat
      421 gagtcaatta tcatagatga aggtactacc aatttgcaga ttgtagatta tataatatct
      481 gtaaacaatt taaaaattat tacaaactct tatccagttc tgtcaaaatt gatttcttac
      541 gagaatcaag gtttgtttga tggagaagtt atatttattg gtggaaaagt aaatttaaaa
      601 caccaaagga cagcatgtcc aatatcaata gatgctatga aaaacttata cgtagataaa
      661 gcttttatat ctagtgaagg tatacttgat agttttggaa ttagtagtat agaccaaaat
      721 aaggcattgt tgtcaaaagc atatataaaa aattcaaagg aaagttttgt attatgtgat
      781 agttctaaaa taggaattgc tagtatgtat aagatagcag atttagagga attagatttt
      841 gttatttgtg atgtagaacc tccgaatgaa tggaaggata agttaaatga gataaatata
      901 acatggatag aagcaaaata aacccttagt tttatataat acaactatgc tcaatacaac
      961 taaattcata aaatatagtc agttcataga ttaagattag aaagtaaatt ttagattgta
     1021 acttaggatt ttatgggaat atgatacaaa ataaaaacaa ctaaatacaa ctaaaaacag
     1081 aaatttaata tatatttaac ctaaatatga tgtataatta acatatgaaa gttatacttt
     1141 aaaagtaaaa acaattaaaa acacaaaaat tctaaaaaga atctatggag gctataaata
     1201 tgaaaaagat atatggagaa aaaataaaag cagtagtatt cgattgggct ggaacaacag
     1261 tagattatgg atgttttgca cctcttaatg tatttataga gatatttaaa agaagaggta
     1321 tagatgtaac aatggaagaa gcaagaaaac ctatgggaaa attaaaaata gaccatataa
     1381 gagaaatgtg tgaaatggat agaataaaga atatttggtc agataagttt ggtaaagtgc
     1441 caactgaaga tgatgtaaat gaactttatg ctgaatttga gccaatgtta tttgagacat
     1501 tagaagagta tacgactcca attcctcatg ttgttgaaac tattgaaaag ttgagaaaaa
     1561 atggattaaa aataggttct acaacgggtt atacaagaga gatgatgaac atagttgaac
     1621 caaatgcagc aaagaaagga tactcacccg attttcttgt aacaccatca gaagtatctc
     1681 aaggaagacc atatccttgg atgtgctata aaaatgctga agctttaggg gtgtctccta
     1741 tgagttctat ggtaaaagta ggagatacaa taagtgatgt aaaagaaggc gtaaatgcag
     1801 gaatgtggag tgttgctgta ataaaaggtt caagtgagtt agggcttaca caagaagaag
     1861 tagagaacat ggataaggaa gaattaaaag ctaagatgag tatagtttca aagaaattta
     1921 aagaggcagg agcacatttt gtaatagaaa ctatggctga gttagaagat atactaataa
     1981 aaatagagaa tgaaactatt aaatcagatt ttgtaccaga aaatgattac atattactta
     2041 caccaggacc actatctact actaaatcag taagagcatc tatgttaaaa gattggtgta
     2101 cttgggatgt tgaatataat aacctagtac aagatgttag aagaagatta gtatctctag
     2161 caacccaaaa tacagataaa tacacaagtg tattaatgca aggaagtggg actttttcag
     2221 tagaagctat aataggaagt actatttcaa aagatggtaa acttcttgta attgcaaatg
     2281 gagcatacgg aaagagaatg aaagatatat gtaattatct taacatacaa tttgttgact
     2341 gcacttttaa agatatagaa gcagtggatt taaatgtagt agaaaattta ttaaaagaaa
     2401 acaaggatat aacacatata tcaatggttc actgtgaaac tactacaggt agattaaatc
     2461 ctatacaaga ggtagggaaa cttgcaaaag aatataacaa aatttatata gttgatgcta
     2521 tgagtagttt tgggggaatt gagattgatg ttgaagattt taatatagat tttttagtta
     2581 gtagttctaa caaatgtata caaggagtac ctggatttgg ttttataata gctaataaag
     2641 agaaattatc taaatgtaaa ggtatagcta aatcattatc tcttgatgtg tatgcgcaat
     2701 gggagactat ggagaaaaat aatggtaaat ggagatttac ctcacctaca catgttgtaa
     2761 gagcttttta tcaagcttta ttagaattgg aagaagaagg ttctgttgaa aaaagatatg
     2821 ctagatataa agaaaatcag tttacaatag cttcaagatt aaaatcttta ggatttgata
     2881 ctttggtgaa tgataatgct caatcacctg taataacaac tttcttatat cctaaaaatg
     2941 caaagtttga atttatggaa ttttatactt atttaaaaga taatggattt gttatctatc
     3001 caggtaaatt gactgatata gatacattta gaataggtag cataggtgaa gtttatccaa
     3061 ctgatatgga gagactagct gatgtaatag aaaaatttat caatagatag aagaagatag
     3121 tgtcttgtag gttacagaat agtgtggaaa gcccactcct ttaggtgtgt gagatgatag
     3181 cacaaacagt gtataaaaag tattgacagt agaatttaaa gacttatatt ttagaagttt
     3241 atacaatgaa tatataaaga ttgagtaata gggatatatt ttatttaaac cacaatctaa
     3301 gaaattagat ttatagttta aataaagcat ttccctatta tttttatgcc tatttataga
     3361 aaataaatga tagttgcttt attaagtatt attatcatac ttgacaacgt aacaatgttt
     3421 tgttatatta ggtttaaaga taaaatacgg taaagaaagg atatcattat gaatgaagaa
     3481 ttaagttcac taagatttga tggtgttgga aaatgtaaag gtggaatttt tgataatgtt
     3541 aatatatcag gaacaggcaa aatagaagga agtgtaaaat gtagtaaatt tgattcttct
     3601 ggttcagcaa aagtttttgg gacaacagaa tgtaatgaat tttttacatc tggtttaagt
     3661 aaagtagaag gaaacatact ggcatcaaaa gttgagatat cgggacttct tagatgtaca
     3721 ggaaatatta attctgttga aattgatact agtggaatta ttaatgtaga aggaagtata
     3781 aaatctgata ttattcatgg agaaggatat ttaaaagcaa atgagaatat agattgtgaa
     3841 aatatcaatt taagtggaat cttggagtgt aatggatttt tgaattgtga agaagttaat
     3901 attagtttaa atggaacttc taattttaat gaagttggag caagtagtat aaatataaaa
     3961 aaggggcatg gagattattg caaaaaattt cgtatgctag aaaaaaagca tatcaacttt
     4021 gaaaaagtgt cttcaaattt gataagttta ggtctattat caattagaaa gtatacagaa
     4081 agtaaattgt ttgcaaatat tatagaagca gatgaaataa gcttagaaaa tagtgaagtc
     4141 aaagttgtaa gaggaaaaaa tattaaaata ggcaagggat gttatgtaga aagtttggag
     4201 tacagtgaaa gtatagagat tgatgaagat tcaatagttg gtgaaataaa gaatataagt
     4261 gaaaaaataa aattaaagaa ggataagtaa taagtaggtt taaaaataaa atttaaaatg
     4321 aggtaataat atggaaaaag ggtctataaa aatacttgga gaaggcttta taagtagtgg
     4381 tgagtatgaa aatataaaaa tcgtgggaag ttctaaatca aaaggaaatg ttaaaagtaa
     4441 cgaagtaaaa gttcttggag atgcaaaatt tgaaggtgac atcaatataa aaaattttgt
     4501 tgttaacgga aaaactatta taagaggcaa tcttaaagca gagcgtgtaa gagtgaatgg
     4561 tcaacttaat atagagggag atgcagaaat agatgactta gttataactg ctcaacagaa
     4621 agtggatggc aaacttaaat gtaataatgt tacaataaga ggacaattaa gtgtaaatga
     4681 aaatatgtat gcacaagata taaaaatcta tggcgaaatg agaaataaat caaatataga
     4741 atgtgaagat ataaaggtat atggagcaat aaaatgtgat ggtcttttaa atggagaaaa
     4801 tatatatgtt tattcaggag gaggttctta ttgtaaagag ataggggcaa ctaatataaa
     4861 aataggaaga agcaaagatg ctttttcatt aattaaaatc attaatattt tttcaggaaa
     4921 gtttaaatgt aatttgatag agggagatac tattgaatta gaagatgcat caataaaaga
     4981 tatacgaggt aaaagcatat ctctagtaga taattgtgat gttggaaata ttgaatacag
     5041 tgataattta tcagtttcag ataattctac tgtaaaaaac agtataaagg tgggctagaa
     5101 aagttatgga agaattaata tctaagaaag atttattgag caaaactaat atatcttatg
     5161 gtcaattata tagatggaaa agaaaaaaca taataccaga ggaatggttt ataaaaaaat
     5221 cagcttttac aggtcaagag actttctttc caagagataa aatactagaa agaatagagt
     5281 taatattgtc tatgaaagaa gagacttctc ttgatgatat tgcaaatatg tttatgaaaa
     5341 aggatattaa taaagaattt gacattgatt ttattctcag taaagaagtt atatgtaatt
     5401 ataccaaaga aatatttcaa aatttatata aaaaagagca aagcataggt aaaaaagaac
     5461 tgcttatatt gagcataata gagaagtttt tagttaaatc tataataact tttgaagagt
     5521 taaaattgat agtgaatata atagaagaaa attttacatc aatatacaat gaaagtggaa
     5581 agatttattt gtttagaaag tttggagttc cattcgtagt aggatgtctg gattataaaa
     5641 aagtaaagtt tgaaaaagat gtagttaaga ttattgagat tgacttgaca aaagaaataa
     5701 atgatataag tataaagtta tcatagtgta ttatcttata taatgtatta taataatata
     5761 cttaaaatac actttattta aatatttagt ttaaagataa gattttatgt gagtatgtaa
     5821 aagttaaaga tagatttaag taggttatag ggattagtaa acaataaaat gaaccatgat
     5881 gaattactat aaattgtatt taaactacat tttctactta tagtaatatc aatggttcat
     5941 tttattgtta ttaagataat acagtattta taaaaatact tgatataaat atagtaaatt
     6001 gtttaaatag aattattgcc ttaatttatt caaataatga gtttttaaaa tctaagaaat
     6061 atccttgtgg atgaagacat acaggacaag cttttggagc ttctttacct atatgtatat
     6121 ggccacaatg tgtacatatc cattgtatat cttcatttct tctgaataaa gtaccttctt
     6181 ctaaatcagt agcatatcta ccaaatctat caccatgaac tttttctatt tcagctatat
     6241 tttcaaataa cttagctata gattcaaatc cttcttcacg tgctgttttt ccaaagtttt
     6301 tatatacatc atcccactct tgaagctcat tatgttgtgc agctttaagg tgttgtgctg
     6361 tcttgtcata taaatctaca gggtatgctc ctgatatact tatattttca ccagcaaatt
     6421 cttttaattt agaaaagaat tgttttgcat gtgatttttc ttggtcagca gtgtaattaa
     6481 ataaagactc tattatatat aatccttctt tttttgctac agatgcagaa atattgtatc
     6541 tatttctagc ttgactctca cctgcaaaag ctcttaataa attttcttta gttttactat
     6601 cttttaaatt catattctac ctctctatca caataaattt ttacaatatt tgattctttt
     6661 tatttattat tccaatagga gatataatta tgctcaattt agtcagcatt tttaaaatta
     6721 tcattaattt tttttaaaat tctaagaaat tcttgtttct cattttctgt aatattatga
     6781 tatgccgtct ctttaacttg tgttgagatg ctattaaaag tatcttctat aagcagtgcc
     6841 ttttttgtaa gctttatata cgtgatacgt ttgtcatttg tacatttttg tctttctaaa
     6901 tatcctaggt ttataagttt attaaccaac actgtaacag tggatttatc ttttcctatt
     6961 ttagctgcaa tttctttcat tgtaagtatt ccattatttt cataaagtgc agttaaaata
     7021 tttccatgag taggtattaa ctcacctaaa ccatttttac tgagtttatt ttcaatgaat
     7081 ttaatcatac tagcctttgt tttacttatg aaatgtacaa tatatttatc ttccataaat
     7141 tattctccaa tctttaatct atttaaatta gtttgacata aaactattta taaactataa
     7201 ttagtttaac ataaaactaa atgtattggg aggaaattta tgaataaaaa atttaaatca
     7261 ctatatatta cagaagaaga aggaaatttt agaagaaata tagttgaaag agaagtggat
     7321 acattgccag aaggcgaagt tttaataaaa gtaaaatatt cttctttaaa ttacaaggat
     7381 gcactttctg caaatggtaa taagggtgtt acgagaaagt atcctcatac accaggaatt
     7441 gatgcatctg gtatagtaga atctagtagt tgtgatgatt ttaaaattgg agaagaagtt
     7501 ttagtaacag gttatgactt gggaatgaat acttctggtg gtttttcaga atacataaga
     7561 gttccttcta gttgggtagt taaacttcct aaaaatcttt cactaaaaga agcaatggat
     7621 tatggtacag cagggtttac agcagcttta tctgtgtata aatttataga ttctgtagac
     7681 aagaatatgg gagatgtatt agtcactggt ggtactggag gagttgctgt aattgcaact
     7741 aaaatattga ttaaattagg ttattcagtt gtagtttcaa caggaaaatt agaagaacaa
     7801 aaagaagtat tactaaactt aggtgttaaa gatgttatac ataggtcaga agtggatgat
     7861 aactcaggaa gacctctgct tagaccaaga tgggctggag taatagatac agtaggagga
     7921 aatactctag caacagcaat aaagacaact aattattgtg gagcagtaac aacttgtggt
     7981 aatgcaggag gagttgattt tacaagttca gtatatccat ttatcttaaa gggaattact
     8041 ttatatggta tagattctgt ggattgccca atgaacttaa gattaaaaat atgggattta
     8101 ttatcaaatg aatggaaatt agatgattta gatgatatgg taaatgtagt atcattggaa
     8161 gagttaaatg atagtataga ttcaataatg gcaggaaaac atgttggaag aacagtagtt
     8221 gatttagaaa aaatataggt gaataaattt ataattagga ttataggttt taagcaaaaa
     8281 tgagatactt gaaatttact tatgagtatc tcattttttt atgtaaaaag atttatgtag
     8341 atgtttgtgt aaaaagatta ttcatgaaca attaattcaa atacatctgg tcttgaatag
     8401 tgaccttctg aatcaaaatc tagtctacta agtactattt tttctaggtc taaatcagct
     8461 attagcattt cttctttatt gaaaatagga cctgctacat attttccaaa agggtcaact
     8521 atacaacttc ctcctggaca cataatttct ggttctgcat ctagctcctt atagtagtta
     8581 aggtcagttg ggtacgtatt tttttcaaca tattgattgc atccaattac aaaacatctt
     8641 ccttctaaag caatatgttt catagttgct tgccactctt ctcttgaatc tgcagtaggg
     8701 gctatgtaaa gttttacacc cttagcataa agagctgttc tagctaatgg catataattt
     8761 tcccaacaaa tcaacgaacc catttttcca tatggagtat caactactgt aagagtactt
     8821 ccatctcctt ctccccaaat acatctttct gttcctgtag gtttaagttt tctgtgttta
     8881 ccaattagct taccttctgg agagaaaaat aagtttgtgc aataaagggt acaatttata
     8941 tcattaccat ctctttctgt tatacccatt gatagatata ctcctgcttc ttgtgctgct
     9001 tttcctaata aatctgttgt agcactagga actggtacag aattatcata gtatcttttc
     9061 caatcttctc taccttccat tgttctactt ccaacaacaa atccaaatga aagacctctt
     9121 ggatatgcag gtatgaatgc ttcagggaaa actacgatat ttgccccttt tctaccagct
     9181 tctttaatta atcctaatgc tttttcaaca gttttttcta aatccattat gactggtgac
     9241 gcctggacaa cagcaattct tacattttta ttttccttgc tcataaaaaa cctcctaaaa
     9301 aatatttaaa ttttttgcat tgaatagatt acatgagatg ttatttgaga actaagatac
     9361 ttatattgtg aataataatg taactgtata tttaaaataa tacatcatga tttaaaaaat
     9421 tacaattgta cagatataat tgagtttaga tatttaaatt taacaactta taatattttt
     9481 tcaattagta taaagcctat taatttgtct tatttacaat gaatagtatt tacttcttgg
     9541 caaacaataa ttaaaaataa aacttttata tgattaaact ctaaagaaaa agtttataaa
     9601 gaagataata taattaagca gaaaataatg aaaaaaattt attaaagtag acaacgtaag
     9661 gagaatttta tgaaagcaaa aatttattac aatggaaata tcatcacaat ggaagattca
     9721 atatgtggag atgctattct tataaaagac aaaataataa aaaagatagg aacaaaagaa
     9781 gaagtatttg ctttgaaaaa taaagataca gaaattatag atttacaagg taagacattg
     9841 atgccatctt ttatagattc acatagtcac ttgatagctt ttgctactac tctaaagcta
     9901 gttcctttag aagatgcaac cagttttaaa gacatagtaa aaaaaataca ggatttcaaa
     9961 gagtcgaata atataaaaaa aggagactgg ataattggat tttcatatga taataatttt
    10021 ttagaagaaa ataagcatcc agataagagt gtactcgata gtgcatcttc agaaaatcca
    10081 atactaattt cacatgcttc tggtcatatg ggagttgcaa atacattggg gttaaaacag
    10141 cttggtgtta ccaatgaaac acgtgaccca gaaggtggac atataggtag agtagaaggc
    10201 agtgaagaac caaatggata tttagaagaa aatgcatttt ttaatgtagc atctaaaata
    10261 aaacaaccat catcaaatga gatttttaat tcaattgaaa aagcacagaa catatatcta
    10321 agctatggta ttacaacagc tcaagaaggt ttaatggaag aaaatcaatt taacatttta
    10381 aaagcaatgg caaatcaaaa taaattaaaa atggatgttg taggatatgt aaatttgaaa
    10441 aaaagtaagt ctgtagcaga taataatagg gagtttataa aaaaatatat aaatagattt
    10501 aaaataggag gttataagat tttcttagat ggttctccac aaggtaagac agcatggttg
    10561 agtagaccat atgaaaattc agatgatggt tattgtggat atcctatata caaagatgaa
    10621 gaagtagaaa agtttataga tatttcacta aaagaaaaga tgcaactctt aactcattgt
    10681 aatggagatg cagcagcaga ccagttgata gatgcgtttg agaaagtttt aaacttaaaa
    10741 gagcaaagta gtgaaaataa tataagacca gttatgatac atgctcaaac agtaagagca
    10801 gaccagattg atgatatgaa agtaataaat atgatacctt catattttgt agctcacaca
    10861 tattattggg gtgatattca tatcaaaaac cttggagaag atagagcgtt taagattagc
    10921 ccacttaaaa caactataga aaagggatta atttacactc ttcatcagga tacaccagta
    10981 atagcaccta atatgctgga aacagtatgg tgtgctgtca atagaattac aaaaaaaggt
    11041 atacaaatag gagaaaatga aaagataagc ccattagatg ctttaaaggg tgttactata
    11101 aatgcagcat atcaatattt tgaagaagat aaaaaaggtt caattaaaga agggaaatta
    11161 gctaatttga taatattaga tgaaaaccca cttacaatcg accctatgaa aataaaagat
    11221 attaaagtat tgcagactat aagagaagga gaagtattgt attctttgaa gtagatgaaa
    11281 gttagatatt ttataatcta taaaaaatag ttttatataa taagttaggt agaattaaat
    11341 aagattacag ttaaatcaac aattatattt ttattaaatt ttaattattg tattgaagat
    11401 ttataagtat aattttattg attctacctt tttaaataac tgtagattta ttaaagttat
    11461 ctatgtacta tattaaacaa ttaagtagtt attaattgca cttattagtt tattaaaaaa
    11521 ctatgtatat tactttgcag taagactttg gaactaaatc tataatagca aatttaaatt
    11581 tattaaatag attaacttat aacatttcat ataaaatata ttgtgaatct gagaaaatta
    11641 ctatttttct ttgagtattt tttgtaagca gttctaaaag attcaatttc atacctatta
    11701 cttgaatcat tcatcaaaat aattctaatt taaaaaa
//
LOCUS       DBGJFU010000115        11628 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.115, whole genome shotgun sequence.
ACCESSION   DBGJFU010000115 DBGJFU010000000
VERSION     DBGJFU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.115"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            85..648
                     /locus_tag="ACOIAS_002952"
     CDS             85..648
                     /locus_tag="ACOIAS_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702646.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            1045..2745
                     /locus_tag="ACOIAS_002953"
     CDS             1045..2745
                     /locus_tag="ACOIAS_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702647.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2915..3532
                     /locus_tag="ACOIAS_002954"
     CDS             2915..3532
                     /locus_tag="ACOIAS_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702648.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     regulatory      3643..3811
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            3893..5086
                     /locus_tag="ACOIAS_002955"
     CDS             3893..5086
                     /locus_tag="ACOIAS_002955"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGZ2702649.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            5104..6396
                     /gene="lysA"
                     /locus_tag="ACOIAS_002956"
     CDS             5104..6396
                     /gene="lysA"
                     /locus_tag="ACOIAS_002956"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGZ2702650.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            6613..7272
                     /locus_tag="ACOIAS_002957"
     CDS             6613..7272
                     /locus_tag="ACOIAS_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702651.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNSNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(7427..8512)
                     /locus_tag="ACOIAS_002958"
     CDS             complement(7427..8512)
                     /locus_tag="ACOIAS_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGZ2702652.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            8703..9419
                     /locus_tag="ACOIAS_002959"
     CDS             8703..9419
                     /locus_tag="ACOIAS_002959"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGZ2702653.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            9477..10055
                     /locus_tag="ACOIAS_002960"
     CDS             9477..10055
                     /locus_tag="ACOIAS_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2702654.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            10449..11321
                     /locus_tag="ACOIAS_002961"
     CDS             10449..11321
                     /locus_tag="ACOIAS_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGZ2702655.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4795 a         1203 c         1991 g         3639 t
ORIGIN      
        1 aaccacattt attatgttag aatatcttat gatattaaat tgtaagtgta taatattatt
       61 tagttactag attgggggga aatgatgaat aaagtctata ttgtaacaac atatacagga
      121 actatattat cttacttaat aaggaatata agtaaaaagc tgtatacaca tgtgtcaata
      181 tcattaaatg aaaatctaaa accaatgtat tcttttggaa ggctaaatcc aaggaatcca
      241 tttattggag gatttgttga agaaaatata aatcaaggat tatatgctat aagaaaaaac
      301 actgtgtgta gggtatattc gttagaagta gataatttac aatatgaaaa tctatataaa
      361 aatataaaat taattagtga ttatagagaa gattactatt acgatacaat ggcattaata
      421 tatttactaa tgaatattca gagacaatct gaatataaat atgtatgttc aaattttgtt
      481 gctgacatgt tagaaaaaag tgagatagac attttaaata aacaagcatt tgaagtaacg
      541 ccaaatgatt tttataattt atctggctta accttagagt atgaaggttt attatcagaa
      601 tataattcta gacaacattc ttatgtatca aaaatcgcaa acatttaaaa acttgattta
      661 gagtttttca tataaaaaaa tattgctaga taattaggct gaagatagta gtttaacttc
      721 gataggatta agaatttgta aatcttagtt agaatacttt cttcagtttt taaatttctg
      781 ctaatatttg tatttttata atatttttgg atatatataa tacagaatag tgcaaaaatc
      841 ccactccttt aggtgcggga tggatagtac aaacagtgta taaaaagtat ttatacatac
      901 acgtataatg ttaaatatag ttgaataagc agaactataa atatataaag atatcttaat
      961 atataataaa tatgataaat atttattttt tgtaaatata tggatataat agtaataata
     1021 acatttttta gagaggagta ttaaatgaaa cgatatctta aaacatatgg agttgcatta
     1081 ataattgtaa taatttatgc atttattaaa ttacctgtac taagattaga ttttacatct
     1141 ttattttcag taggaataat tttctttgtt gtagcaggaa tactagatat gatgttagat
     1201 agaaatgaaa gagcatctaa aatggctaaa tataattttt caatagcaat agctttgctt
     1261 gtgtatattg tagtagttcc ctttataact tctacacctg tacttcatgc aaaatcatat
     1321 agagaactat tgggaaaagt aactgaaagt aaatttactg atgacattag ccctgtcagt
     1381 gtaaatgaca ttcgtcttgt agatgaagat atggcaatga aattaggtga caaaaagctt
     1441 ggagaagttc ctgcaatagg gagtgtatcc aaattaggaa aatttcatat acaaaatgta
     1501 gatggagagt tgtactgggt agcgcctctt gttcacagag acataattaa atggataaca
     1561 tctctaagtg gtacaagtgg ttatgtaaaa gtatcagcta gtaatcctca agacgtccaa
     1621 ttagttcaag aaatagatgg aaagccaata aagatagttt accaaccaga agcctactta
     1681 catcaggatt tacaaagaca tttatacata catggtattg taaatgttgg tatgacagat
     1741 ttcactcttg aaataaatga tgaaggaaga ccttattggg ttgtaagttt atatgaacat
     1801 aaaattggct atggaggagc aaatgcaaca ggaatagcca ctgttgatgc agaaactgga
     1861 aaaataaatg tttatgatgt taaaaacacg cctaaatggg tggatagaat acaaccacaa
     1921 agctttgtaa cagaccaaat taaagactgg ggtgtttatg ttaatggatt tttaaactct
     1981 gttatatcag aaaagggagt tttagtacca acagaaggta catcactagt ttatggcaat
     2041 gataacaggt cttattggta tacaggtata acatcttcag gtggagatga atctacaatt
     2101 ggatttatgt tagttgattc tagaacaaaa gaagctagat tatataaaca accaggagct
     2161 actgaaacgg ctgcaatgaa atcagctgaa ggtaatgttc aagaaaaaaa ttatgaagcg
     2221 acgttccctg taatgtataa tatattgggt cagccaacat atgtatcatc attaaaagat
     2281 aaggctggtc ttgttaagag agttgcattt gtatctgttg aagattataa tgtacttgga
     2341 ataggtgaag ataagaatga agcattaaga aactataagg atgcattaga atctaagggt
     2401 aatgatttaa aacttgataa tgatttaaaa gatgaggtat tagaaggcac agtgacaaga
     2461 ataagcccag atgtgcgagg tggtaacact aattattatg taactttaga ttcaaataaa
     2521 gatataatat ttagggctac ttcaaaagta tctagcgaat tgccattgac tcaggttggt
     2581 gataaagtca aaatatcgta ttcaaaagaa gaatctggtg taatagaaat gtcagaattt
     2641 gataacctaa atattgcaaa ctttgaagat aaaaaagcaa aagaaaaaac aggaactgat
     2701 gaaaatacag attccaaaga ttctgaaaat caaggtaatg tataatttaa ataaaaagat
     2761 tgataatagt atttaacata gtgatagcta atttttatag aatttaattc tataaaaatt
     2821 agctatttta ttttaattct ttgtaaattt aatgtcataa aattaatata atattagtac
     2881 aactttgcaa ataatacttc aaggagaatg tactatgacg aatatggaaa aagagatatt
     2941 acttttaatt agcagggtta gatgtgttac agagtctcaa tttagaaaaa tttatggaga
     3001 ccaaaaaaga tacaataaaa aaaattttaa aaaaactttg agaaaaatgt gtagtgaata
     3061 tacactaaaa aaatatcctt gtgatgtgga ttattataat tataaagacc tctcttatgt
     3121 atactattta aacggtagta aactttttaa gggagatgat ttaataaagg ctctagtagg
     3181 atcagaatta gttgttagaa tcaatactgc aggttgtgat gtaaaaagat tttaccgaaa
     3241 tgttagtgta gatgatgtga aatacgattt atttattgaa tatacagacc aatttggtaa
     3301 tataaagcaa gttttagttg atgttgactt aaataatgaa atagacattt ctaaatacga
     3361 aaatctttct aaaaaaatag ataaatctac aattccattt tttaaagtac caaacgtttt
     3421 ggtagttaca ccagataatc tatccgaatt aaatgattta gagagatatg ataatgagta
     3481 tagcataaat tttatagatt taggattaaa taaactattt aattgtatat aagctaactt
     3541 tagaaaagaa aactatcgat ttgccacatt ttgtaaaata attgttgcaa tattatatag
     3601 aatatgataa aactatatta ataaattaat taaaaataat gagcctaggg tagaggtagc
     3661 tgtattcaat agtattcaat aggagctggt aagcgttgat aattgatgaa agggtatata
     3721 gccgaagaaa ttttgttgac ggacaaattt tctggactta tgcagaatat gcataaggct
     3781 gtcactttta aagtggtgag ctacaaggtt acttggtcta tgaaatttac acgtccaaag
     3841 tatacctttg ggcgttttta tttatcaaaa tttagcttga gggggagaaa gcatgagtat
     3901 aattgttcaa aaatatggtg gaagttcagt agcagatact gaaaaaataa aatcaatagc
     3961 agaaaatata atagaacgaa gaaaagaaaa tccacaaatg gtaatagttg tgtctgccat
     4021 gggaaaatcc actgatgaat atataacttt agcaaaggaa ttgagtaatg aacctagtaa
     4081 gagggaatta gatgctttaa tgtctactgg tgaaatgatt tcagcatctt tattatcaat
     4141 agcattaaat gcacttggat gtaaagctat aagttataat gcttaccagt taaatataaa
     4201 aacgagtggt cttcatggaa agtctcaaat tgatgatata aacgtaaaca gaattaaaaa
     4261 tagtttagat gaaggaaatg tcgtgatagt aactggattt cagggaatca acgaagatgg
     4321 tgatgtaacc actcttggaa gaggtggttc agatacatct gctgtagctc ttgcggttaa
     4381 attaaatgga aaatgtgaga tatatacaga tgttgatgga atttatttta cagacccaag
     4441 aaaatattca aaggctagta aacttgatga gattgaatat gaagaaatgc ttgagttagc
     4501 gagtttagga gcacaagtaa tgcattcaag aagtatagag cttgcacaaa aatatggtgt
     4561 tgaaatctat gtcggacgta catgtggtac agaaaaagga acatatataa gagggggaaa
     4621 agacatgaaa ttagaggata aagtaataac aggattagct actagtgatg atgacagttc
     4681 tataacaata aaagatttta aagctgaaaa tatatctagc ttatttgaag atatagcaac
     4741 tataggtata agtgtagata tgataagcca aactgcacct attttagata agataagtgt
     4801 atcatttaca gttccaaaag aagagttggg agaatgtaaa aagatagtat ctaaatatac
     4861 agatgaagaa catgtagtga ttgataataa tatcactaaa ttttctttag taggtcttgg
     4921 tatgaaaaat acttctggag ttgctgctaa agtatttaag atattcaatg aaaatggtat
     4981 aatgataaaa cttataacta catcagagat tcgtataact tgtgctataa attctgatga
     5041 taagcaagta gctatagaaa aaatagcaga agtatttaat atataaaatt tggggaggct
     5101 attatgaaac ttcatggaac tatgactgta aataatgatg aattatatat tggaggagtg
     5161 agttgtttag aacttagtaa aaaatatcaa actccacttt atgtatttga tgaagcttta
     5221 gttagacaaa attgtagaga atatgtagaa tattttaagg ctagagaggg aaaaaataag
     5281 gttgcttatg cagggaaagc ttttttacct ctttacatgt gtaatcttat aaatgaagaa
     5341 caaatgtgtt tagatgtagt ttcaggaggc gagttatata cagcttataa atcaaatttt
     5401 cctatggaaa aagttttctt tcatggaaac aataagacta aagaagaaat aaacatggga
     5461 ttagatttag gtgtaggtaa atttgtggtg gataattttt atgaattaga tttgcttgaa
     5521 gcattatgtg aagataaagg taaaactcaa gagatatact ttagaataac tcctggtata
     5581 gaagctcata cacatgatta tataaaaact ggacagatag attctaaatt tggatttgct
     5641 ttagtaaatg gagatttatt tagagcaata gaaaaattaa gtgaatataa aaatattaaa
     5701 cttgtgggtc ttcacgctca tataggttca caaatatttg atgtagaacc atacatagat
     5761 acagtggata taatgatgaa cttggtaaaa gaaataaaag aaaaattcaa catacaacta
     5821 acagaaattg acctaggtgg aggagttggc gtttattata gcgaaggaga taaacctaaa
     5881 actataaaag agttttgtga aaccataatc actaaagcag aaacttcatg taaagagctt
     5941 ggaatagaag tacctacatt ggtaatagaa ccaggaaggt caatagttgc aaatgcagga
     6001 agtacattgt atacagtagg ttctataaaa gatataaaag atgttagggt ttatgtaagt
     6061 gtagatggag gtatgacaga taatgtaaga ccttcgttgt accaagctag ttatgagtgt
     6121 agtattgtga ataaaattaa tcatgaggct atgaatcatg taacaatagc tggaaaatgt
     6181 tgtgagagtg gagatatatt aattggagat ataaacataa tggatattaa aagtggagat
     6241 atacttataa caacatcaac tggtgcatat ggatattcaa tgtcttcaaa ttataataaa
     6301 ataccaagag cagcagtagt atcagtattt gatggaaatg ataagataat ttgtaagaga
     6361 caaagctatg aggacttatt agctttagag atataaaatg gagaagtata taataaaaat
     6421 taaatattaa agtttattat gagcagattt gctaggatat gtatctctaa attaattttt
     6481 ggtttattgt atcgttcgta gcatatctgc ttattttttg ttttactaac aaatttcttg
     6541 taataaatat aaaatatttt aaaaacttta taattatgat aaaatttaaa gtatatgaca
     6601 aggagggatt taatgagagg aagagtaatt ttattaatat cgatattatc tatatttctt
     6661 gtaggatgtt catttaataa aaaagatgaa ataaatttag tagagcaagg aaaaacatat
     6721 ttagaaaaac ataactataa aaaagctatg gaatcattat cctctgcact ggaagaagat
     6781 agtactaatg agaatgcaag agctatgtat atgcaagcta tgagaatgtc aaatatgacg
     6841 gagtttgaag aattaaaaaa ttatgagggt gcaatcaagg aacttaaact tatagagaaa
     6901 atcaaaaatg gttcctctgt tattaaagaa gaggcaacta aaaagaaaga agagttaact
     6961 aaattagaaa aagaacaaaa cgaagcagag gaacaaagaa agaaaaaggc aaaagatacg
     7021 gctggagaag ataggtatag agttgaatca gatgcaagga aatcttctta tagtggaagt
     7081 agtaaaaata ataaatctag ttctaaaaat aatgataaaa aatcagatag taataagtct
     7141 gacaatagta acagttcttc tcaaaatcaa gaaaagccaa ataatagtaa tccaacacca
     7201 gcaccaactc cacaagatac tactggtggt tcagatagtg gtggaaattc tcaaaactca
     7261 aataatcaat aagtataaaa tatatgcata aatttaatga acaaattata tattataaaa
     7321 aatatctctt agcaattttt tcttactaag agatattttt tgagaaataa tttaataaat
     7381 aacagttaaa ttttaaacta aaaattaaag tcacagtaga gataagctat tgtaaacgat
     7441 tatctatata tttatcaaca taagttttta caacagcagc tattggtact gaaagaaaaa
     7501 ctcctatgaa accaaataga gattgtccca ccaaaatagc agatattacc catatagctc
     7561 ctataccaac ttgattaccc ataacttttg gttctataaa atttccatct aattgttgca
     7621 atataaatag aaatgcaatg acccatatag ttttttgtgg actatataat atagtcatgg
     7681 ctattggagg gattgcacca ataaaaggac caaaataggg aattatatta cctaagaata
     7741 ttattgaagc taaaaataaa gcattttcta ttctgataac aaattgaaat cctaaaaatg
     7801 caattatacc aataataaga gagtctaata ttctaccaag taaataatca tagaaaatat
     7861 catgagtagt cctaaaaaat tctataatgt tatctgcctt gtttctagca gtagaagaat
     7921 atagaaatct cttaaatccc aatgcaattt tttctttgtc atatataatg tatatagata
     7981 gtataaatcc cattgtcata ttaaatagca tagaagatat actaagtgta tagattaaga
     8041 agtttttagg aattttacca acaaaattac ttgcttcttt taataaatta tctaaactat
     8101 gttgtatatg aggaattaaa gtttttagaa aatatatgtt tgacatattt ttttctataa
     8161 aactatctat ataatcagta tacataggca tttcatttat caatctattt aatgtattag
     8221 ctattgatgg aattaataac ctaatggcaa aggctaaaat aaaacctata aatatataag
     8281 ataaaaaaat ggctaaaagt ctatgtgctt tgaattttat ctcaaaaagc atcacaagtg
     8341 gatttaaaag taaagcaagt aaaatagcta ataaaaatgg gctaaaaaac tttaagaatc
     8401 cttcaatact agatataaag tctgatggag tattaataaa tttaaaaaat aaaattccaa
     8461 taaacacgac tattaaataa tattttatat tttcctttga aataataatc acttccttaa
     8521 aatagtgttt agtcagctat tacatattaa tagtatgtga aaagaaatta caattattta
     8581 ggaaaattaa ataatttttt tgcttgtttt taattaaggt ctagtatagt ataataatgt
     8641 agcaaaatta acattgataa aaatattaaa cacacataat ttaagataag gaaggattaa
     8701 gtatgagaat taataagtat atagcttctt gtggaatagc atcaagaaga aaagcagaag
     8761 aaataattct tggaggaaga ataaaggtaa atggaagtgt agtaaaagag ttatctttta
     8821 atattgatga agaaaaagac atagtagaat ttgataataa aaaagtaaaa ccaagtgaaa
     8881 attatatata tatagtatta aataaaccag agggatatat aactacagta aaagaccaat
     8941 tcaatagacc cagtgtaata gacatactta aagatgtaag ggaaagagta tatccaatag
     9001 ggagattaga ttatgaaact agtggtttat taatacttac taatgatggc gatttaacat
     9061 ataagcttac ccatccaaag catgaaattg ataaaatgta tgtggcaagt gtgaagggaa
     9121 ttataagtga tgatgagata agacaattcg aaacaggact aaaaatagaa gattacacaa
     9181 cggcacctgc caaaataagg gtaactaaag aaaataaaga aaaaaattat tcagtgtgtg
     9241 aaataactat acatgagggt agaaatagac aagtaagaaa aatgtgcaaa gctataaatc
     9301 atccagtttt aaatttaaga aggatttctg tggggaaaat agtattaaaa gacaccaaag
     9361 ttggagaata tagataccta acagaagatg aaataaaata tttaaaatca ataaaataag
     9421 ataaaaataa attgagaaaa aatgaaataa agttaatagt agggagaaat agtattatgg
     9481 catatgcaaa gtatttaact aaaattacag atataaataa attttattta gaaaatatta
     9541 taaatgaagg tgacatagta attgatgcta ctatgggaaa tgggtatgat acaaggtatc
     9601 tagctgagaa ggttggagaa aagggctttg tatatgcttt tgatatacaa gaagaagcaa
     9661 taaagtctac aagaaagaaa ctagagaaag aaggatatac agatagagta aaattgatat
     9721 ttgatgggca tgaaaaaatg aatcaatata taaaagaaga agtttcatgt gttttattta
     9781 atttgggtta tcttccaaga gcgaaacatt caattataac caaaccagat acaactttaa
     9841 aggctataaa gttaagttta gaaatgttaa aagaaaatgg agtaataagc attgccatat
     9901 atacagggca tgatggaggt caagaagaaa aagattgcat atataacctt gtcaataatt
     9961 taaatcaaga tgaatttaat gttttagaat ctaagttttt aaatcaagtc aataacccac
    10021 cacaattgat tttaattgaa aagaaaaaag cataataaat actaaaaaac ttgacaagat
    10081 tgtattaaga gttaatatat atagtgtgac ctattaaaaa tactttaaat aataagtata
    10141 caacagttta ttctcaaaac aaaatataca tctaaatatg caaaataaga tagataactt
    10201 ttaaatgagt aaaaattgaa aaatttaaaa gaaatattgg ataaatatta aaaaatgcaa
    10261 ggaaagatgg tataataaat ttaatcgaaa atactatgtg aagtataagg tacagaaaag
    10321 ttagaatttt atgagtttaa gaagctatga taaaaactaa gatgaaattg agataaaaaa
    10381 taaaattttt agagaaaaag acagtgttaa taaaacaaaa tagtttatat aactaaaagg
    10441 agaaaaaaat ggaagaaaca aatgaaatga aggacagtaa acatttgata tcaaatatac
    10501 aatctcaata tacgagattg agtaagggtc aaaaacttat agcacaatac attttaaata
    10561 attatgataa agttgcattt atgactgcat gtaaattggg tgaaactgta ggagtaagtg
    10621 agtctacagt tgtaagattt gcaaatgcat taggttatag tggatatcct aaattacaag
    10681 ctgcacttca agaactcata aaaaataaac taactacagt acaaagagtt gaaatggcac
    10741 atgattattc cgatgatttt gcaatactaa acaaagtttt aaaaagtgat atagataata
    10801 taaggtctac actagaagaa attgacgaga gagcgtttaa agaagcttct aacaaacttt
    10861 taagagcgag aaaaatatat atacttggaa tgaggagttc ttttgtagta gctcaatatt
    10921 taggttttta cttagatata atacttgaca atgttcatat aattagaatg gatatgggag
    10981 atgcttttga gcagattgta agaataaatg aagaagatgt aatagttgct attagtttcc
    11041 ctcgttattc taagaaatct tatcaaattg tcaattatgc taaagaaaaa ggagcccatg
    11101 taatatcatt aacagatagc ttatttgcgc cagtagcatc acttgcagat aacactcttc
    11161 tagttaagag taatatggct tcatttgtag attctctagt tccagctctt agtatttcaa
    11221 atgctcttgc tatatctgtt gggatgaagg aaaaagaaga tattaaacag cattttgatg
    11281 atttagagca aatttggaaa agatattctg tgtatgagta aagtttgtgt attaataagg
    11341 ctatgaaatt gagaatcata gccttattaa taaatactag attatcactt aaaaaataaa
    11401 ttttcgttta aataatacaa ataaatattt cgagactcaa aacataaaga tgtattctga
    11461 atatatcttt actacatcaa ctaatttatt aaagaaagca tttcccttca tggagagtgc
    11521 tttttttatt tctctaaaca atatcatttc cttaattaac aattataaaa ttaattttat
    11581 ttgtatttaa gtatataata tcttattaaa attctattaa aatatgta
//
LOCUS       DBGJFU010000116        11627 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.116, whole genome shotgun sequence.
ACCESSION   DBGJFU010000116 DBGJFU010000000
VERSION     DBGJFU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.116"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(41..943)
                     /locus_tag="ACOIAS_002962"
     CDS             complement(41..943)
                     /locus_tag="ACOIAS_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGZ2702656.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            1298..2476
                     /locus_tag="ACOIAS_002963"
     CDS             1298..2476
                     /locus_tag="ACOIAS_002963"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGZ2702657.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTITVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQSKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            2485..3141
                     /locus_tag="ACOIAS_002964"
     CDS             2485..3141
                     /locus_tag="ACOIAS_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HGZ2702658.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIIEGGNL
                     "
     gene            3141..3806
                     /locus_tag="ACOIAS_002965"
     CDS             3141..3806
                     /locus_tag="ACOIAS_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HGZ2702659.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            3911..4687
                     /locus_tag="ACOIAS_002966"
     CDS             3911..4687
                     /locus_tag="ACOIAS_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HGZ2702660.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            4749..6083
                     /locus_tag="ACOIAS_002967"
     CDS             4749..6083
                     /locus_tag="ACOIAS_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HGZ2702661.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            6579..7475
                     /locus_tag="ACOIAS_002968"
     CDS             6579..7475
                     /locus_tag="ACOIAS_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HGZ2702662.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPEDNNNSDSTNKPD
                     DNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYF
                     SHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFS
                     KIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(7867..11406)
                     /gene="nifJ"
                     /locus_tag="ACOIAS_002969"
     CDS             complement(7867..11406)
                     /gene="nifJ"
                     /locus_tag="ACOIAS_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGZ2702663.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         4002 a         1863 c         1783 g         3979 t
ORIGIN      
        1 ataaaaattg atttatttat ttgttatttt aataacattt ttaagagttc tttttactaa
       61 agaaatctat aataaattta aatacatgtt ggtctacatg gctataacgt tcttttttag
      121 gtctacataa tataactttc catgtaatag gatgattaaa aggcttttct ataattttgc
      181 tattttcaaa aaaatcatga actggtgatg gtagaattgt tataaaatca gaatttgtgg
      241 ttgatagcaa caaaaaatcc catgaaccag acattatata tctttttaac ttaacatttt
      301 catcagtaaa tttttgcatt agcttatgat gtatcataaa agtaggatta aatattgcaa
      361 gtaactgctc attcaaatcg ttccaatcta tcttatcctt cttagcaagt ggatgattta
      421 tatttaaaaa tgctgtaagt tcatcttctt gaagcacatg ttcagttact acacttttat
      481 caattttatg tggatgtaac aatactgcat aatctaattc ctgtaagata agcattctat
      541 ttaagtcata tgcacccttc tctactatat caaattctat atctggatta tttgcaacta
      601 actgtgcaac tacatctgaa aatgctattc caagtataag tggaggtatt cctattctaa
      661 cttttccttt aaattgaact gaatcttctc ttaaatcttc catcatattt ttgtattcat
      721 ttatgatatg ttctgcattt acataaaatc tttctcctgc tggtgttaac ccatttaacc
      781 tgcctttata cctttcaaat aaatatacat cttcactttc ttcaaaattt tttataattt
      841 gacttaatgc tggttgagat atgtgaagtg tcttagatgc tattgataaa ttaaaaccag
      901 atttcactat ttcaacaaaa tatttcaact gttttatatc cattttaaac ccccttcccc
      961 aaaagtataa tttagattat attttttctt ctaatgatat tttataggaa aatcaacata
     1021 atttcattaa tattttaaca aaatattttt ctaaatctac tataaatttt tatcctataa
     1081 atttttctta tacactatat aaaaaattag acttagacaa aacgcttttt tcgccgtaat
     1141 ctataattag aaacaggaaa acctaaagca ttaggaataa aagtaattga aatacttaaa
     1201 aacaagcttg tattaaaaaa gtactttatt atagtgtttc ctaatttctt tattcagaaa
     1261 atttttaatt atcattcaag aaaggtaact attagatatg aaagatgtag taatcgtatc
     1321 agcagtcaga acacctatag gaagttttgg aggtgtattt aaaaatactt cagctgttca
     1381 acttggaact atcactgtta aagaagcgat atcacgtgta ggacttaatt tatctgaaat
     1441 agatgaagta ataattggaa atgttcttca aacagggctt ggacagaatg ttgctagaca
     1501 aattgctata aatgcaggta taccaaattc tgttccaagc tacacagtaa ataaattatg
     1561 tggttcaggg cttaaaagtg tccagctagc tgcccaatca atcacatctg gtgaaaatga
     1621 tgtggtaatt gcaggtggta ctgaaaatat gagccaagct ccttatatcg ttccaactgc
     1681 tcgctttggt agtaaaatgg gtgacataac catggtagat agtatgctaa cagatggcct
     1741 tattgatgct ttcaatcagt atcatatggg aataactgca gaaaatattg caactaaatt
     1801 tgaatttact agagaaatgc aagataaatt ggcattagaa agtcaaagta aggctgagaa
     1861 tgctataaaa aacaaccgct ttaaagaaga aatcgttcca gtagatgttt taatacgtcg
     1921 aggaaaaata gaaactattg ataaagatga atatcctaaa ttaggtatga catttgaagg
     1981 tttagctaaa cttaaacctg cattcaaaaa agatggtact gtaacagctg gaaatgcctc
     2041 aggtattaat gatggtgcgg ctatgttaat actaatgagc caacaaaaag cagacgaact
     2101 aggtattaga ccacttgcta aaataaaatc ctatgcttca gctggtgttg aacctgaagt
     2161 catgggtact ggtccaatcc ctgcaactag aaaagcactg aaaaaagctg gacttagcat
     2221 caatgatata gacttaatag aagcaaatga agcgtttgct gcccaagcat tagctgttaa
     2281 aaatgaactt caaatagatt catcaaagtt aaatgttaat ggtggtgcaa tagcattagg
     2341 acatccaata ggagcaagtg gagctagaat acttgttacc ttaatttatg aaatgcaaaa
     2401 acgtaaagtt gaaactggac ttgcaacact ttgtattggt ggtggtcaag gaatatcaat
     2461 ggtggtttca cgataggagg aaatatgaat aaaatagtta gcattgatga agctctatct
     2521 catgtaaaag atggagatac aataatggtt ggtggattca tggccaatgg ttcaccagaa
     2581 aatttaattg attatttatg tagtaaaaat ataaaagatt tgacactaat ttgcaatgat
     2641 actggcttta ttgacaaagg agttggcaaa atggttgtaa ataagcaatt taaaaagata
     2701 attgcttctc atgtaggatt aaataaagaa actggaagac aaatgaataa caatgaaaca
     2761 gatgttgagt tagttcctca gggaactctt gctgaacaaa tacgtgcagc tggttatggt
     2821 ctaggaggta tactcacacc gactggtatt ggtactcttg ttgaggaggg aaagcaaaaa
     2881 attgttgtcg atagcaaaga ataccttctt gaaaaaccaa tttatgcaga tgttgctctt
     2941 ctatttgcat caaaggtcga taaagcaggt aatctagttt ataaaggctc tatgaacaat
     3001 tttaataatt taatggcatc tgctgccaaa attacaatag ttgaagcaga tgaaatcgtt
     3061 gaaattggtg atattgaccc aaatgaagtt aatacaccag gtatttttgt gaattacatc
     3121 attgaaggag gtaatttata atggataaat tagaaatgca agaatatatt gcaaatagag
     3181 tatctaaaga attaaaagac ggagccgttg taaaccttgg aataggtcta ccaaccaaag
     3241 tagcaaatta tattccggat aatgtaaacg ttatcttaca atctgaaaat ggctttttag
     3301 gtttaggttc agccgaagat ggaaattcaa gtgatgaaac tattgtaaat gcaggtggtc
     3361 aaccagttac tatacttcct ggaggatgtt tctttgatag tgctacttct tttggaataa
     3421 tcagaggagg tcatgttgat ataactgttc ttggcgcact tcaggttgac aaatatggta
     3481 atatagcaaa ctatatgatt ccaggtaaga tggttccagg tatgggtgga gctatggacc
     3541 tagtaactgg agctaaaaaa gttattgtag caatggaaca tacttcaaaa ggttctgcaa
     3601 aaatattaaa taattgtact cttccactta ctgcaacaaa tgctgtagat ttgatagtta
     3661 ctgaaatggg agttatggaa gtaacatcag atggaatttt acttaaagaa ataaatccag
     3721 ctttcacatt agatgatgtt atttctgcta ctgaggctcc tttaatattg tcagattcat
     3781 taaatggaaa aatatctgtt gtttaaaata attaaaacta gaaaataaaa atctcttaga
     3841 cgtattacat ataaatttgt attgcttaaa atattaatat aaaaataaaa taaaattgga
     3901 ggaaatgaaa atggttaaag ataaagttgt attcgtaaca ggagctgcaa gtggaatagg
     3961 taaacaaata gctgaatcgt tcttaaaaaa tggtgctaaa gtaatgttct ctgatataaa
     4021 tcaagaagct ttaaataata ccataacaga gttacaaaaa aaaggttatg attgcatgag
     4081 tgtaaagtgt gatgtaacta aggaagaaga aattaaccac gctatagaca aaactgttga
     4141 aaagtatggc agattagata tattaataaa caatgctgga cttcaacatg tttctatgat
     4201 agaagatttc ccaactgata aatttgaatt tatgataaaa ataatgctga cagcaccttt
     4261 tatagctact aaaaaagcat ttcctataat gaaaaaacaa ggctttggtc gtataataaa
     4321 tatggcctca atcaatggtg taataggatt tgctggaaag gctgcttata actcagcaaa
     4381 acatggactt attggactaa caaaagttgc tgctcttgaa gctgccgatt ctggtataac
     4441 agttaatgct ttatgccctg gatatgtaga tactccactt gtcagaggtc agttcaatga
     4501 cctttcaaaa actcgtaata taccattgga aaatgtctta gaggaagttt tatacccact
     4561 tgtaccacaa aaacgcctat tagatgttca agaaattgcc gactatgcta tgttcttagc
     4621 aagtgataat gctaagggta ttacaggaca agcttgctta ctagatggag gatatactgc
     4681 acaatagtta ttatatttag ggcttgaaga aagtattctt caggtccttc tactaaggag
     4741 ggatatttat gagtattatc gcaccaattg gtataattat tggtatcata gctattatat
     4801 atttttcagt taaagaaatt catataacta tagctgctcc aatagcaact ttaatagttg
     4861 ttattttaaa taaaatggat gttgtaacat caatgctagg tgctgacaaa aacaattata
     4921 tgggtgcact tggaaactat ataatgagtt attttgcaat attcttatta ggctcaatac
     4981 ttgctaaatt tatggaagaa agtggagcta ctgtatcaat agctgatttt attttaaata
     5041 aatttggctc taatcatcct tatagagttt tagttgctat atttatagta agttttatat
     5101 taacgtatgg tggtataagc ttatttgtag ttatgtttgc ggtcattcca cttgcaagaa
     5161 ctctatttaa aaaacttgat atttcttgga accttattca gataccactt tggcttggta
     5221 ttgcaacaat tactatgact ataattcctg gaacaccagc cattcaaaat gttataccta
     5281 ttcaatatct aaatacgtct cttactgcag ctactatacc aagtattgta ggtagtatag
     5341 gttgtgctac gtttggactt gtctatatga aatatgctct aaacaaaagt attagaaaag
     5401 gtgaaactta tgcaacttat acttcagaat tagaagaaga atctttagat agagaattac
     5461 caggattttt tgcaagtatt tttcctctat tagcactcgt aattatagct ataactggaa
     5521 gtacttttgg ttcagaattt atgaaaaaaa atgtaattta catagcttta atagttgcaa
     5581 ttcttttatc tgctttttta ttccataaat ttctatcatc taagatacag acattgagca
     5641 taggtgctag tggttctata ggtcctatat ttgctacatc ttctgcagtt gcatttggag
     5701 cagtaataat ggctgcacct ggttttgatg tatttagcaa acttataatg tcaataccag
     5761 gtagtccact tataagtctt actgttttga cttcaacaat gtcagcaata acagggtcct
     5821 cttctggtgc attaggaata gtaatgccaa actttgcaga ttattattta agtactggtc
     5881 taaatccaga attaattcac cgtgttgcta caatagcttc aaatatacta actatagttc
     5941 cacaaagtgg tgtttttctt acattcttaa gcttatctaa gttaacacat aaaactggat
     6001 ttaaagaaac tttcattgta gttgcagttg gttcactaat agcagaaatt attgtaatta
     6061 cattaggatt actaatgtat taatataaaa caaattaaaa ttcataatat gaaaggtggt
     6121 gtctcaaagt gaacttttta cttaatgaga cactcttttt atatgaaata acatatgcct
     6181 atcatttaaa caataaaaaa ggccttatct ctattttgag acagaccctt tttatttctt
     6241 aaagatttaa ataattataa tttgttgcta atacgaaagc atctactgaa taaatttaac
     6301 caaataaaaa aaaatttatc ataactataa atttacgtta tggaattatt ataaaaatat
     6361 tattttctct aactaagtaa ttccatacta tacaggcttt taatacaaat tgtcaaacac
     6421 actgaaatat acctatttat tccatatata tcttttttac atatctttac agtaaaaaaa
     6481 gtcttaatct tatgttagta tagattcacg ggtaaccccc tacactaaag gttacaaatc
     6541 agtaacattt ttagaatttt tagattaaga ggtgtttaat gatgagaaga aatataccaa
     6601 caaaattaaa aactatatta gctttaggag tgttaacttt agcaacagct tcaagtgctg
     6661 ttactgcaag tgcttcatca cttgaaaata atgtaaatat aagtaacaaa agtttagtat
     6721 atagtaatac taaatacaag ttttctgcaa aaccaaattg ttctgtaaaa ccagaactta
     6781 agcctgataa agataactct tgtaaggata agaatcataa tgacaagaat tgcaacaaca
     6841 ctaataaacc tgaggacaac aataattcag actctactaa taagcctggt aacaataaac
     6901 ctgagtctaa taaacctgaa gacaacaata attcaggctc tactaataaa cctgataata
     6961 ataaacctga gtctaataaa cctgaagaca acaataattc aggctctact gataaacctg
     7021 aagacaacaa taattcagac tctactaaca aacctgatga taataacaac tcaggctcta
     7081 caagtgaaag tttttcagct taccaaaaag aagttgttga cttagtaaat atagaaagag
     7141 ctaaagctgg attaaatcct cttactcttg attcttctat atcaaatgtt gctacaaaaa
     7201 aatctcaaga tatgattgat aacaactatt tttcacacaa ttctccaact tatggttctc
     7261 catttgacat gttgaaaaaa tttggaataa gctataagac agcaggtgaa aatatagcta
     7321 tgggtcaaaa aactcctaaa gaagttgtaa atgcatggat gaattctgaa ggtcacagaa
     7381 aaaatataat gaatccaaat ttcagtaaaa taggtgttgg agtagcacaa aaaagtggtg
     7441 gttcaattta ttggacacaa atctttgtag gataaaataa tctttttcca tacatatcaa
     7501 aagattatct ctaagtgata aaaaacacaa atgagatact cttttgtatc tattttaaaa
     7561 ttatcaaatc caaatataca tactataaat attatttgtt ctcttaattt tagtgtgtag
     7621 agcaatatat aaaaagtatt ggcaagatga aaatggaaaa cttaaaaaga tatttaatag
     7681 gtaaaaacag gatcttaata tcttcataaa aaaaggctgt ctcaaaatag agataaggcc
     7741 tttttcattg ttgaaatgat taaaatatat ttgatttcat acaaaaagag tgcctcatga
     7801 actaaaaagc tcactttgag acactctctt ttatcaattc tatataagca ttataatact
     7861 ttaatattat tcagctaatt ttttgtatcc ttcatatctt tctttagcat cagcttcagc
     7921 tttagcaaat aatccatcag cttcctctgg gaattgttta gctatagcag aatatcttac
     7981 ttgtgcaagt atgaaatctt tgaagcttgc agttggttct tttgaatcta aagtgaatgg
     8041 gtttttacct tcagctttta cttctggatt gaatctgtat aaatgccagt atccgcaagc
     8101 aacagcttgt gcttcgttag ctacagttct tcccatacct tcttttaatc catggtttat
     8161 acatggagca taagctatta ttaaagatgg cccatcatgt ttttcagctt ctataacggc
     8221 tttgataaat tggtttttat cagcacccat agcaacttga gctacatata cgtttccata
     8281 tgtcatagcc atcattccta aatctttctt tctagatttt ttacctgaag cagcaaactt
     8341 agccatagca gcagctggag tagctttaga agcttgacct cctgtatttg aatatacttc
     8401 tgtatcaaat acaagtacgt ttacatcttc accagatgca agaacatggt ctaatccacc
     8461 gtaaccgata tcatatgccc aaccgtctcc acctagtatc cattgagatc tcttaactag
     8521 ataatctttt ctatctttta tagcttttac taactcttta gctttttcat ctgtacaatc
     8581 acaagctggt ttttctaata attcaacaac cttagcactt gctactttag aagcttctcc
     8641 atcatttcta gaatctaacc actctgtaaa taccgcttta gcatcttcac agatatccat
     8701 agaaactaac tctgtcatag catctactat tttatttctt atttgcttaa cagctgtgta
     8761 cataccaaat ccatattcag cattatcctc gaataaagag tttgcccaag atggaccttt
     8821 accttcatgg ttacaagtat aaggaattga tggtgcagaa gctccccata tagaagaaca
     8881 tccagtagca ttagctatca tcattctatc tccatataat tgtgtaacta ctttaatgta
     8941 tgcagtttct ccacatccag cacatgctcc tgaaaactcc attaatggtt gtctaaattg
     9001 acttccttta acagtgtttg caggcataac atcacctttt ggagctacct catcaggatt
     9061 tactgcaaat gcccaattat ctaactctgt atcaagttga gtatctattg gcttcataac
     9121 taaagctttt tctttagctg gacatatgtc agcacagttt ccacatcctg tacagtcaag
     9181 tggactaagt tgcattctat attttaatcc ttctaatcct ttacctaatg cttttttagc
     9241 ttcaaatcct tctggagcat tagctaattc ttcttcagta actaatactg gtcttataca
     9301 tgaatgagga catataaaag aacattggtt acattgaata cagttatcta ttatccattc
     9361 aggaacatca acagctatac cacgtttttc ataagctgca gtaccacaag ggaatgttcc
     9421 atcttctata ccattgaatg tacttactgg taagttgttt ccttcttgtg cagtcattgg
     9481 tcttagtata ttttttataa attctggttc tttaacttct cctttatctg catcaacagc
     9541 attttcccaa gaagctggaa cttcaacttt aactaaagca tttattcctg catcaacagc
     9601 agcatagttc atgttaacta ttttttcacc ttttttacca taagctttaa ctatagaatc
     9661 ttttaagtat ttaacagcat cttcttctgg tattatttca gctaatttaa agaatgcaga
     9721 ttgcattatc atgtttattc tgtttccaag acctatttct tgtcctagtt taacagcgtt
     9781 aactgtatag aattttatat catttttagc tatatattgt ttcatatgag ctggtaaatg
     9841 ttcttcaagt cctgcttggt cccatatagt gttaagtaca aatgtaccac cttttttaag
     9901 accttttaat aaatcatatt gataaacata tgattgttta tgacaggcaa tataatcagc
     9961 ttcatctatt aaataagtag ctcttattgg agtatcacca aatcttaagt gagacatagt
    10021 tataccacca gattttttag agtcataatc aaagtaagct tgagcatatt tttcagtatg
    10081 gtcaccgatt attttgatag cttgtttatt agctccaaca gtaccgtcag aacctaatcc
    10141 ccagaactta catcttatag ttcctttaga agctattttt attggctcag atggagttaa
    10201 agaagtatta gttacatcat caactatacc tactgtaaat ccatttttag gttgttctga
    10261 aactaagtta tcaaaaacag tttttatatc tgaaggagta gtatcttttg aacctaatcc
    10321 gtatcttcca cctattatca taggagcatt ttcttttcca tagaatacat cacgtacatc
    10381 taagtataat ggttcaccag ttgaacctgg ctctttagtt ctatcaagta cacatattct
    10441 ttcaacagta gatggcatag catctaagaa atgtttcatt gagaatggtc tatataaatg
    10501 aactttaact aaaccatatt tttctccctt agcatttaag taatctatag tttcttctat
    10561 tgtttctgtt actgaaccca tagctattaa tacgtattta gcatcttctg ctccatagta
    10621 atcaaataaa ctatgttttc tacctgttaa attactcatt ttttccatgt atttttcaac
    10681 tatacctact atattttgat agtatttatt agaagcttct ctagtttgga aatatatatc
    10741 tgggttttga gcagtaccac gagcaactgg atgatttgga gataaagcat tatctctaaa
    10801 tgcttgaact gcttcaaagt ttaataaact tgcataatct tcattttcta gtaattcaac
    10861 tttttggatt tcatgtgaag ttctgaatcc atcaaagaaa tgtataaatg gtaatctacc
    10921 ttctattgca gctaaatgtg caacaggtgc tatatcagct acttcttgaa ctgaaccaga
    10981 agctaataat acacatccag tttgtctagc agccataacg tcttggtgat ccccaaatat
    11041 tgataaagct tgagatgcta aagcacgagc acttacatgg aatactcctg gtaacaactc
    11101 tcctgcaact ttatacatgt taggtatcat taataataaa ccttgagatg ctgtaaaagt
    11161 agaagttaaa gctcctcctt gtaaagaacc gtggaatgtt cctgatgcac ctgcttctga
    11221 ctgcatttca acaacattaa ctgtttgtcc aaatatattt tttcttcctt gtgatgccca
    11281 ctcatcaact acttcagcca tagttgaaga tggtgtgatt ggatatatag ctgctacatc
    11341 tgtaaacgca taggcaacat gagctgcagc tgtatttcca tcaagtgttt tcataaactt
    11401 agccataata tgtatcctcc ttgatagcta caattgtact tgtttataac aagtctattt
    11461 ttttatattt acatataatt tacacaacaa aactataaac acacatatag tcgcgcaaat
    11521 actctatctt ctaatataat actacagatt agataaaatt cctttgaaaa ttgtaaatta
    11581 attacatatt taagtagatt ttttttaaaa ttaaatatgt taagttt
//
LOCUS       DBGJFU010000117        11536 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.117, whole genome shotgun sequence.
ACCESSION   DBGJFU010000117 DBGJFU010000000
VERSION     DBGJFU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.117"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            126..437
                     /locus_tag="ACOIAS_002970"
     CDS             126..437
                     /locus_tag="ACOIAS_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGZ2702664.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      530..622
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            722..1516
                     /gene="thiD"
                     /locus_tag="ACOIAS_002971"
     CDS             722..1516
                     /gene="thiD"
                     /locus_tag="ACOIAS_002971"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGZ2702665.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1540..2328
                     /gene="thiM"
                     /locus_tag="ACOIAS_002972"
     CDS             1540..2328
                     /gene="thiM"
                     /locus_tag="ACOIAS_002972"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGZ2702666.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2351..2983
                     /gene="thiE"
                     /locus_tag="ACOIAS_002973"
     CDS             2351..2983
                     /gene="thiE"
                     /locus_tag="ACOIAS_002973"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGZ2702667.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKQYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3122..3424
                     /locus_tag="ACOIAS_002974"
     CDS             3122..3424
                     /locus_tag="ACOIAS_002974"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2702668.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3490..4239)
                     /locus_tag="ACOIAS_002975"
     CDS             complement(3490..4239)
                     /locus_tag="ACOIAS_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702669.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4233..5195)
                     /locus_tag="ACOIAS_002976"
     CDS             complement(4233..5195)
                     /locus_tag="ACOIAS_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2702670.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5199..5657)
                     /locus_tag="ACOIAS_002977"
     CDS             complement(5199..5657)
                     /locus_tag="ACOIAS_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGZ2702671.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            6013..6369
                     /locus_tag="ACOIAS_002978"
     CDS             6013..6369
                     /locus_tag="ACOIAS_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2702672.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6441..7157
                     /locus_tag="ACOIAS_002979"
     CDS             6441..7157
                     /locus_tag="ACOIAS_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702673.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7150..7974
                     /locus_tag="ACOIAS_002980"
     CDS             7150..7974
                     /locus_tag="ACOIAS_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702674.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            7986..9185
                     /locus_tag="ACOIAS_002981"
     CDS             7986..9185
                     /locus_tag="ACOIAS_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702675.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEDSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            9194..10006
                     /locus_tag="ACOIAS_002982"
     CDS             9194..10006
                     /locus_tag="ACOIAS_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702676.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTELWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            10017..11285
                     /locus_tag="ACOIAS_002983"
     CDS             10017..11285
                     /locus_tag="ACOIAS_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702677.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         4618 a         1192 c         1813 g         3913 t
ORIGIN      
        1 tgtagagtat gttttataca tatattataa ttaattcttt gaagattgca tattcaaaat
       61 acacaccatt ttttttaatt ctataatata atatttatat atttaatgtt aggatgtgat
      121 ttaaggtgta ttttacatat attataagat gtaaagatga ttctctatac actggatata
      181 ccagtaatat agttagaaga atgaatgaac ataaattagg aattaattca aaatacacaa
      241 gagcaaaggg atttgaaaaa ctagaagttt attttgtgac caacacaaaa agcaatgcta
      301 tgaagttaga atattatata aaaaaattaa ctagaaataa aaaattgtct ataataaaaa
      361 acccaagtat acttataaat ttaattgata ataaagaaga ttatattatt ggtaaggaaa
      421 tagaacaatt aacataaata tttatagtaa cattataatg ggtgcatatg gtgtaaaaac
      481 ttgacaataa atactattgt aatataatga agttataaat atagtaaatt aaaatagcta
      541 ggggagccaa gagctgagag gtataccgac ccttacacct gatctggata ataccagcgt
      601 aggaaagctt agtaaaaatt caagtatgta ttatttgtga tatatgctat ttctaagcta
      661 tacctattgt aggtatagct tttttaatta agttatttaa attaaacttt aggaggcaac
      721 aatgagtaat tataaaatac caacacttac aatagccggc tctgattcat ctggaggagc
      781 tgggattcag gctgatttga aaacgtttag tgcaattggt acttatggta tgagtgtaat
      841 aacagctata acagcacaga atactaaagg agtttttgct gtagaggatt taaataagaa
      901 aataataaaa aaacaaatag aggcagtctt tgaagatata cctccaagag ctgtaaaaat
      961 aggaatggtt tcaagtcctg agattatact tgaaatagtt gagaatctaa agaaatacaa
     1021 tccaaaatat ttggttgtgg acccagtaat gatatctaaa agtggatatt atctattaaa
     1081 gccagaggca aaagaaaact taattaagta tttgatacca cttgcataca taattacacc
     1141 aaatatacca gaggcagaag aaataacagg aataaaaata cataatgtag atgatatgaa
     1201 gagagtagga gaagaaatac tacaacttgg acctaaattt gtacttatga aagggggtca
     1261 tttagatgga gaagcagtgg atattttagt tggtaaaaat atatttaaag tttataaaag
     1321 tgaaagaatt gataaaaaaa atacacatgg aacagggtgc acattatctt ctgcaataac
     1381 atcttatcta gcattaggat atgaaataac agaagcagta aatctaagta aaatctatat
     1441 aacagaagca ataaaaagaa gttttgatat aggtcatgga gtaggaccag tacatcattt
     1501 ttataaattt gagtaataaa aaaatattgg aggtagaata tgtataattt aattaaagat
     1561 gtaaaaaaat taaatccatt ggtaattcat tatactaata atgtaactat aaatgattgt
     1621 gctaatgtaa ctttagctgt aggagcaagt cctcttatga gtttttcata tgaggaagta
     1681 gaagaaatgg taagtgtagc aaactctgta gtaataaata taggaactat gaactcaaat
     1741 atgttggatt tattcctatt agctggaaaa gcagcaaata agtacaataa acctgttgtt
     1801 ttggacccag ttggagtatt tgcaagcaaa gcgagagcag aacttacaag tagactttta
     1861 aatgaagtca aatttagtgt tgttaaaggg aatgtatcag aaataaagtt tataggtggc
     1921 tttaatgtaa ggggtaaagg tgtagattca tttgatgaag aagaagattc tactgagata
     1981 ataagaaaga ttgctgaaaa gcttgaatgt gtagttgtag caactggaaa aatagatata
     2041 ataactaatg gtaaaggtac atataaaata aacaatggaa ctgataaatt aaaaggaatt
     2101 acaggaactg gatgtatgac tgctagcttg attgcaagtt ttatggctgt tactgagaat
     2161 atattagaag cagcaacaat gggtgttctt acaatgtctt taagtgggga attagctaat
     2221 ttaaataatc caccaatagg tacatttaaa gaaaatctta tgaatgctat ttatcaaatg
     2281 gatatagata ctttaagtaa aaattctaat attgaatttt taaattaata aagagtaagg
     2341 gagacttaaa atgattgata aagaatcttt aaaaaaatgt ttaaagttgt atttagtaac
     2401 tgactctgaa atgcttaaag gtagagactt ttataaatgt cttgaagatg caatatcttc
     2461 tggaataaca actgtacaac ttagagaaaa aaatgcttct ggaagagagt ttttaagaaa
     2521 agctatgaaa cttagagaaa taactaaaca atatggtgtt aagtttataa taaatgatag
     2581 agtggatata gcacttattt gtgatgcaga tggagtacat gtaggacaga gtgacattga
     2641 tgttagagag gttagaaagc ttataggaaa caataaaatt ctaggagtat cagcaagaac
     2701 tttagaagaa gctatttgtg caaaaaatga tggcgcagac tatttaggtg tagggtctat
     2761 attcacaaca tctactaaat tagatgcaaa atcagcttcg tttgaaactg ttaaagaaat
     2821 taaagaaaaa gtagacatgc catttgtact aattggaggt ataaatttag acaatataga
     2881 taaacttaaa tgtttagaaa gtgacgggta tgctataata tcagctatat taaaagctga
     2941 agatatctct aaagaagtgg aaaagtggac attaaaaata taaaattggc tctggtagtg
     3001 aaaaaaatgg aaaaatttga tataatctaa gagtaaaatt taagcaatta taaatgataa
     3061 acttttctac atatactttt taatcaagat atatgtagat ttggttaaaa aggagctata
     3121 aatgattagg atattgttag tgtgtgttgg aggcatgtct tctaccctat tggtaaataa
     3181 aatggagaag gatgcaaaaa aaagaaatat agactgtaaa atatgggcag ttggagaagg
     3241 agatataaaa tcggagcttg ataactttga tattttactt cttggaccac aacttagatt
     3301 tatgcttgat gacgtaaagt ctatagttgg agatagagct ccagtatcta taatagatat
     3361 ggtaaactat ggcacttgta atggtcatgc tgtgttaaat tcggttttag aaatattaaa
     3421 ataaataata aaagtggatt ctatataata gagtccattt ttattatttg ttattaatag
     3481 gcttaaaact tagcttacca tttctttttt taatctccac atagcaagtg aaaaacttga
     3541 acagcacaat attaaaaata gtaggataac aggcagtata tctccaataa gaatagtctc
     3601 accaaatatt aagctacgta aagcgttaat aacatgggta aatggattga gcatcactgc
     3661 tacttttaat cctccagata aactttctat agggaaaaga gctgaactta aaaagaaaat
     3721 aggaagtaca attgcagtca taattgtttc ataaactatt tcatttggca ataataaact
     3781 tatagaataa gctagacttg ataaaaagaa ggctgtcata aatatcagta ctatcataag
     3841 taggattcca ccaatgccag attctatcct tactgagaag aatatactta ctatacacaa
     3901 aatagttact tctatgaaag aaactaagat agcttctaat aattgaccaa ggacgattga
     3961 atacctactg ataggtgcaa ttaagactct atagaaactt ccactgttct tcattataaa
     4021 gtttataata ccaccactac tacaagaaga aaacacaacc agtacaatga tacctggtag
     4081 tataaaagca gtataattat tgatatttat attattcata gtttggtttg caatagaact
     4141 ataaagcaca agccaaagaa aaggttgtaa tatagtaact acaaatgaga ttggattttt
     4201 aaaacgccac ttcatactac gccataagat atttaacatt cccattcctc ctttaagctt
     4261 tttttagaac ttgtaagtgc taaaaataca tcttcgagac ttggctctac aatttctatt
     4321 gcatcaaatt ctatatcatg ttctaaaagc catttattta ttaaagtaaa agcagttcta
     4381 ctatcgttta cacttataaa aattgaattt tctcgtacgc tcataaactt tactaaacca
     4441 gtacttttaa ttgaatcttt atattttttt atatcttcag tattatgaaa agttatacgt
     4501 agtatatttt gtctaatgta actacgtaaa cttgatggtg ttccttgtgc taaatccttt
     4561 ccattcttca taatacaaat attatcactc aattgttcag cttcttcaag ataatgagta
     4621 gttagaaata tagttgtgcc atactcatct ctaattttta ataacatatc ccacatagat
     4681 tttcttgaat cgacatccat accaacagtt ggttcatcta aaaataggat tttaggagat
     4741 gataccatat tcatagctat gtccaatcta cgttttacac cccctgaata tgatgaagta
     4801 ggatatttta agtaactaga taagtcaaac ttatcaatta aagagtctat tctttgtttt
     4861 gcaatctgtg gttctacttt atacatttta ctttgaaata ccatattttc cataagggat
     4921 aaatgttcat caatagagat ttgctgtgct acacaagcaa tttgagtacg tatccaagaa
     4981 ggattatcta ctaaatcttt tccaaacatt gttacatttc cagatgtagg tttgtaaaag
     5041 gtagtcaaaa tatttataag agaagattta ccagcaccat taggaccaag taaagaaaaa
     5101 atttctccag catttacatt aaagcttaga ccatttaaag ctttcacacc atttttatat
     5161 tcttttacta aattgtctat ttcaatcgct aacatatatt attcctcctc tttaagtgga
     5221 aatattatct ctgtaatata gttatctggg tttcctttta tcaacatacc tggtccttta
     5281 ataaatactt ctctccaagg agtttgtatt tttatattat tttcatgaat atatttaaat
     5341 atcatttcat acgtttttgg caaattacta taagagcctg tgtgagtaaa aaataaagct
     5401 tttgttttag gaaattcttt tgtagttata cccatacgat ttggagtttc agcagttgga
     5461 acacataatt ccatttctgc aacaccagtt ttttggtcaa catcatagta gcaaaagaat
     5521 ggtgcaccgt tagactttcc ttttatagct ttaaatacat ttggaaaata ctttgttgcc
     5581 tcagtcatag gtccattata acgcatggta gccactgtga ctgattcgat atttttgatt
     5641 tcaattttgc aattcatttg tttacctcct aatgtgtgtt catattctta ctagattttt
     5701 aagcattaat acatataaga ttttgattag ttgaaaacat ataatcttat ctcgtttgct
     5761 tgttttcaag tctaacataa cataaaatat atatcctctc atttgatgct ccattttgta
     5821 aagttattat ttaagcttta ctttagattt tttagcaaat taaaataaag caatttataa
     5881 aataagtatt tacaaattaa gtgtattaat cgtataatac acttaatagg atactagata
     5941 aagtctgaaa taaaggcgta tttttttaaa gtaagtgtac tatcgtatta atacactaga
     6001 ataggaggaa tcatgaattt tgaattagat aatacaactc ccatttattt gcaaatagta
     6061 aagtatataa agagacaaat agtaacagga gagttgaaac caggagagac aataccatca
     6121 agaagagaaa tggcacttaa tctaaaagta aacttaaata cagttcaaag agcatataag
     6181 gagatgggtg atatgaatat aataaatact tttaaaaatt atcaaagcag tgtaacagtt
     6241 gatgaaaata tattaaaaaa tttaaaatta gagcttataa atgaatcact atctgttttt
     6301 atagaagata tgaaagcaat aaatgttagc aaagaagaag ttttaaaaat aatagaagat
     6361 aagtattagt tttttaacaa atagaaaact tagtttagaa agtttattta taagcatatt
     6421 ggatgtggag gatagtaaat atgatagaaa taaagaatgt cagcaaaaca tataaaagga
     6481 tgcaagggct taaaataaga aagatagaag ctttaaaaaa tgtaagtttt aatatagaaa
     6541 agggaaaaat aactgcatta cttggtataa atggtgttgg taaatcaact atgttaaaag
     6601 caatagcagg acttataaac attgatagtg gagaaattcg tatagatgga gaaaaaataa
     6661 atgaaaaagt atacaataaa ttggcttttg ttccagatgt acaatctcac tttagcaata
     6721 caacaataaa agaaacattt gaatttatgg aaatttttta ttcaaagtgg aataaagaaa
     6781 aatcaaaaga aatgatggac atatttaaat tggatgaaga tgaaataatt gataacttgt
     6841 caaaaggaaa cattgcaaga gtaaaattaa tacttggatt ttgccaagac ccagagtaca
     6901 tacttcttga tgaaccattt acaggaattg atttgtttaa gagggaagag tttataggag
     6961 taatagcgca atatatggag gaaaatcaag ccattatcat aactactcat gagatagtag
     7021 agatagaaag tttagttgat gaggttgtaa ttcttgatga agggcagatt ataacttcat
     7081 ttaatgcaga agaattaaga gaaagagaag gtaaatctat attagataag atgagggagg
     7141 tatataagaa tgaataaact tctatatcta ataaatgtag atttgagaag aagcaataag
     7201 ttttatatag cttatatatc atttttttca ttaattacac ttagtttaaa tttattcgaa
     7261 attaataaat ttaaaaattc taaatttatt atgaacttag caatagaaga ttttggaggt
     7321 atattttatg gactagggat acttagtaat cttggtttta tccaaagtat attgttcttt
     7381 gggatattgg gactttttat atactatgtt tttatgtgga aaagagagtt cttttcaaat
     7441 aatcaaagta tttatacact tatgatgtta cctcaaaata agtttaagat ttgtatatct
     7501 aaatcaatag ccttgataac aatgatatat ggttttttga taagtcagac agcaacatta
     7561 tttatatgta aatatatatt taatttcatt tttagaaata tgcctataat caatatgaac
     7621 tttgccaaag atttatatta tttaaacttg aagagtatac caattgattt tatctctttt
     7681 atggctattt atgtattttt actacttata gctatttcta ttgtaaattg ctgtatatta
     7741 tttatctttt cctatataaa taaattttat gtaattttaa tcactttgct gattatagca
     7801 ggcttatatt tttcaacaat atatataaat ataatcactt tatttagaaa atattataat
     7861 cttataggat ttcaatttaa caatttagta gcagggttat taagtttttt aattataatg
     7921 tttattctaa atggaatatc ttatttgcta attaaaaaga aagtatctct ttaaggaggg
     7981 gtggtatgaa attaaaatct aaaatatcta tacttattat atttttgact ttagcaatat
     8041 attttagtat aggtttatta aataatgaag ataatttaaa gataaaaact ataaaaggtg
     8101 actctaatga attagaccat ataaacataa attttggata tgctttgaat ggatatataa
     8161 attcatatta tacaataaat ggaagcaaca aaactaaaaa gactgaatta ataaaactac
     8221 aaaaggatag taagaacaca aataatttaa ttgatgtatc tagtggttca gatttaattg
     8281 ctcatgttgg atttgaaatg aatgaggagt caaaagatat aaactttgtg atagatgaga
     8341 aaaaagaaga tttagaaaaa ggtgtatttc catcagaaga tagtgataaa gaaattgaat
     8401 atgaaaaatt agatatgaat aattttacaa ggacaagata taaagtagat aaaagtacag
     8461 atgattatta tgcaaagata ctatcatcaa aaagatataa aaaatactta tatgtatctg
     8521 tactttatgc tacaagtgat tcatcagaaa ataacaaaga atattcaagt actatagata
     8581 taataaaaat taatccagaa aataaaaaaa tcaagactat aaaaagtata aatttaagta
     8641 attatttttc cgaaaaatac caaaacatag agaacatagg tgtaagttat gctagcaaat
     8701 atgaggataa gatgtatatt ttagtaaatg tactaaatac aaacataggt gataatagta
     8761 attctaaatt tcatttactt gagtataatc ttgaagaaaa taaggaaagc ataatggaat
     8821 taaaaactga tagcaaaatg gctttagcaa aagcaaagtt agaagctaat aaattatata
     8881 tggtaatgtc gaatgattat gataatagtt caattacaga gttaaattta cttacctata
     8941 atatattaga taaaagctta gatgaagata aatatgtttt agataataga gaaaaaagag
     9001 gaattagaag tattgaaatt gattcaaata aaatcgttgc atatacatca aggctaaact
     9061 ctatattatc ttgggatgat aaatttatgc tatatataat aaataaaaat tctaaggaaa
     9121 ttgtatatga aggagaatta caatttaaca gttcaaatag tatgttagaa tttgaaataa
     9181 agtaggtgag ttgatgaaag atttaataag attattcgaa tttgaattaa aaagaaatat
     9241 aaagaattat atatttataa taatttgttg ttgttctttt gtaattttaa acatagtaaa
     9301 aaatttaaat gactataatt acataataga aaatgcagtt aaaagtgaac agttaacaaa
     9361 aatcggaaat acgatagaaa cggtaaatgt tacaggtttc tcaagcttta gaaatataat
     9421 gggttctaca gaattgtggt ttatgtttgg tataattgca tgtatttgct atgctttttt
     9481 tatttggtat agagacttta atggtagaag taaatctata tatacactta taatgctacc
     9541 taaaaataga atcaatattt atatatctaa attattaaat atattatttc tagtgtatag
     9601 ctatacagta gtacttacaa tatctttgtt tattgcaagt aagttgttac ctagacatat
     9661 gcttggtaat gtcacaaact atggttttgt acaagaaaca atatatgagt taaaaatgtt
     9721 acttccatat agctttgaaa tactatttgt agagtatata tttttattga taggatttgt
     9781 atctgtagta tttacatcaa ttcttataaa taaatctatt tataaagtaa gcactttaat
     9841 aagttttcta tttttagtta tagaaatttt agtgtttata atttctatag aatttattat
     9901 accttacgaa tacagtgatg tatttactgt tctatattca agtataaaca tatttgtatg
     9961 tagtcttata tctaataaac ttttgaagca aaagattgac ttttaggagg gatattatga
    10021 aagcaaaaaa tataggaaaa tatacaatat gttttataat agtatgtttt ttaatgatta
    10081 ctatgacctt aatattgagt tattttaata aggataaact atctgtaaaa actttaaaag
    10141 gcaatgtaaa agatataggg agagtaagtg tagtatatac acctaatata agcaaatttt
    10201 cttatgagga ggtaattata agtaaaaatg gagtaagaaa taatacaaac ccaaaatcaa
    10261 gaattagaaa tgattattta aatattgaca aaaaatacag tgactttgtt aatgataaag
    10321 gaaaagaatt tatatataaa gatgatttga atataggaga agcgtatacc tctgttaagt
    10381 ctgatttagt acaaggaatg aatgctttaa atgattgtat aaaggtttca aataaaaatt
    10441 taaaaagtgg ggtagaagaa aattttgata ttaatttaaa taagtctgat tttaaagaaa
    10501 atagttggaa aaccttattt aacaaaatat ataaaggtaa tttatatatg gtaattagtg
    10561 aaactaatat ttcaggttct aaagtcaata atatttgtat attaaagata gatataaaaa
    10621 ataagtctta taaattatta aatagattta atttaggatt acagaatgga gaacaacaaa
    10681 atatcaatga aattaaattt tctattggaa gtaaattata cttagaaata gtaaataata
    10741 aaagtaaaaa tggatatatt gaaggacatg atagttactc taactttatt atatatgata
    10801 tagaagataa cacttttaat ataagtaata aatttatgga taatctaaac aaagaaaaga
    10861 atgcaaatcc aaaggagatt ggcttattaa gtaaaactaa gctagattat atggtaaaaa
    10921 acaataaatt gaatcttata gttacggata tgagtaattc cataactaat cttgtttatg
    10981 atattaattc taatgagatt aaattagaag attatgagat acttgattta aaagtagatt
    11041 ataatgaagc atcatctgga gttaggaaag ttaagctact ggatgataag atatattcta
    11101 tttcaggtat taagaacagt atatcaggag aattaaaagg atttaaaggg attgttgatg
    11161 tagtcggaac taagccagta aaatttcaag tatttgatat gaaacttaaa aagaatattt
    11221 atgaagctga gctaataaat ggagatataa gtttacaaga aaagttattt tttgttgcaa
    11281 attaagtgtt agaattaatt ttagaacttt aaacttttat atgtatcaca gataagtata
    11341 agtaagggag tgtctcaaaa taaacttttt agctcatgag acactttttt tatatgaaat
    11401 caaatatatt ttcattgttt agatttattt tgataatgaa aaaaagatta tctttatttt
    11461 aagaaagaca tttatttata attatataca aaaatatttt ttaatgtttt tgtgtataat
    11521 tataacaatt taattt
//
LOCUS       DBGJFU010000118        11458 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.118, whole genome shotgun sequence.
ACCESSION   DBGJFU010000118 DBGJFU010000000
VERSION     DBGJFU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.118"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     regulatory      184..227
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            328..1296
                     /locus_tag="ACOIAS_002984"
     CDS             328..1296
                     /locus_tag="ACOIAS_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HGZ2702678.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            1298..1861
                     /locus_tag="ACOIAS_002985"
     CDS             1298..1861
                     /locus_tag="ACOIAS_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGZ2702679.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            1869..2558
                     /locus_tag="ACOIAS_002986"
     CDS             1869..2558
                     /locus_tag="ACOIAS_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2702680.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            2685..3413
                     /locus_tag="ACOIAS_002987"
     CDS             2685..3413
                     /locus_tag="ACOIAS_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HGZ2702681.1"
                     /translation="MMFDRVKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            3427..4101
                     /locus_tag="ACOIAS_002988"
     CDS             3427..4101
                     /locus_tag="ACOIAS_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGZ2702682.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYIFKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            4127..4963
                     /locus_tag="ACOIAS_002989"
     CDS             4127..4963
                     /locus_tag="ACOIAS_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702683.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            4979..5650
                     /locus_tag="ACOIAS_002990"
     CDS             4979..5650
                     /locus_tag="ACOIAS_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702684.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            5640..6872
                     /locus_tag="ACOIAS_002991"
     CDS             5640..6872
                     /locus_tag="ACOIAS_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2702685.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            7022..7339
                     /gene="trxA"
                     /locus_tag="ACOIAS_002992"
     CDS             7022..7339
                     /gene="trxA"
                     /locus_tag="ACOIAS_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGZ2702686.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            7356..8234
                     /locus_tag="ACOIAS_002993"
     CDS             7356..8234
                     /locus_tag="ACOIAS_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGZ2702687.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            8504..8872
                     /locus_tag="ACOIAS_002994"
     CDS             8504..8872
                     /locus_tag="ACOIAS_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGZ2702688.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            8875..9246
                     /locus_tag="ACOIAS_002995"
     CDS             8875..9246
                     /locus_tag="ACOIAS_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HGZ2702689.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(9391..9846)
                     /locus_tag="ACOIAS_002996"
     CDS             complement(9391..9846)
                     /locus_tag="ACOIAS_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702690.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(9885..11150)
                     /locus_tag="ACOIAS_002997"
     CDS             complement(9885..11150)
                     /locus_tag="ACOIAS_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HGZ2702691.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         4620 a         1199 c         1891 g         3748 t
ORIGIN      
        1 ccacatctta ttaaagttgg ttcataattc taaataaact ttaataagat acattaaaat
       61 ataataaatt cttttatgct ataaaattta ttttatagct attaaatcag atgtttaaaa
      121 attttttaac agtctatagt ttttaagagt ctatagtaag tagcctaaaa atatgatttt
      181 acatctgtac ttgttcgaga actccaagta taaaaaacta aggaattata tagtggcagt
      241 atataattaa tacccttaga cacacctttt tgaaagtgga aaaaagatgt gtagttctcc
      301 tcgaccacaa atcaaggagg ataaaaaatg gaaaagagaa aagtaataat tgattgtgac
      361 ccaggaattg atgattcttt ggcaattctt ctggctttaa actcaccaga gctagaagta
      421 attggaatta ccacatgttg tggaaatgtt ccagcaaata taggtgcaga aaatgcacta
      481 aaaacacttc aaatgtgttc ttcactaaat attccagtat atataggaga agaagcacca
      541 ctaaaaagaa aacttgtaac agctcaagat acacatggag aagatggtat tggagaaaac
      601 ttttatcaaa aggttgtagg agctaaagca aaaaatggag cagtggattt tataataaat
      661 actttatata atcatgaaaa agtatcaata atagcacttg caccacttac aaatatagct
      721 aaagcactta ttaaagataa gaaagcattt gaaaatctcg atgagtttgt atctatggga
      781 ggagcattta ggattcatgg aaattgctct ccagtagcag agtttaatta ttgggtagac
      841 ccacatggag cagattatgt ttacaagaat ttatctaaaa aaatccacat ggtaggttta
      901 gatgtaacta gaaaaattgt acttactcct aatattattg agtttataaa tagacttgat
      961 aagaagatgg caaagtatat aactgaaata actagatttt atattgattt ccattgggaa
     1021 caggagggaa taattggctg tgtgataaat gaccctctag cagtagcgta ctttatagat
     1081 agaagtatat gtaaaggatt tgaatcatat gtagaagttg tagaagatgg aatagctatg
     1141 ggtcagtcta tagtggattc tttcaatttc tataaaaaaa atcctaatgc aattgttcta
     1201 aatgaagttg atgagaagaa atttatgtac atgtttttaa agaggctttt taaaggttat
     1261 gaagacatta tagactctgt ggaaggagtg atatagtgtg aaaagaaaaa tatgtgtaaa
     1321 aactattaca cttatagctt ttggtatttc tataaatata ataggcgctt ttatagctat
     1381 gggattaaga ttacctgtat acttagattc aataggaact ataatgatag cttcactttt
     1441 aggacctaaa tacgctgttg ttacaggtgt gtttggaagt ttaataagtg gaattacgtt
     1501 tgatgtgtat tctctctatt ttgcaccagt tcaaatatca accggattac ttgctggaat
     1561 agcatttaaa aagaattttt taaggggttt aaaaactcca ctaggagtac ttttatttgc
     1621 cattccaaca tctataataa gttcaataat ttcagcattt ttatttggag gtatgacctc
     1681 ttcaggttca tcatatatag tgcaaatctt gaaagtgtta ggactggggg atgtttttag
     1741 tgtgtttgta acccaagtat ttactgatta tggagataag ctgttagcag ttgttttagt
     1801 gaatttagga ttaaatgcag ttccaaaaac tttaaaggta tcattgactg gaggaaaata
     1861 gatagaaaat ggatagatat agtgagataa caaataaaaa tcaaagagaa attgttctgc
     1921 ttaaaggatt tccttgtata tggggaaagt gttctttttg tgattatata gatgataatt
     1981 ctaatttaga agaagagatg aataaattaa atctaaaggt tttaaaaaac gtaactggga
     2041 aatatggagt tcttgaagtc ataaattctg gaagctgttt tgaattacct aaggatactt
     2101 tagaaaaaat aaagtgtatt ataaaagaga aaaatataaa aaaattattt ttagaaagtc
     2161 attggtctta caaaaatagg cttaaggaaa tgagagagta ttttgaaatt cctgtggtat
     2221 ttaaaattgg agttgaaacc tttgacaatg attttagaaa taatatttta aataaaaatg
     2281 caaattttaa gactccacaa gatgtaaaag aatattttga ttcaccttgt attatggttg
     2341 gaataaaagg acaaacaaaa gagatgatag ataaagatat agagattatc ctaaatactt
     2401 ttgaaaaagc gacagtgaat gtttttataa ataattcttc tagtataaaa agagatgaag
     2461 agttagtgaa atggtttgtt agtaagtata aattcttgga tgaaaatcca aatatagaag
     2521 ttttatacaa taatacagat tttggtgtag gagattagca ttaatttagt ttaaatttaa
     2581 aattgaaaag tttcttatgt ttttgaaaaa gcttctaata gttagaagct ttttttgtag
     2641 agttttatgt tacaataaga aaaaaggaat taaggagcaa ttaaatgatg tttgataggg
     2701 taaaatggaa taaattggat tataatgaac ttttagaata tatgatgtct atacaagata
     2761 taaaatatag agattttaat aaaaagctta tacctggaac agaaaatata ataggtatta
     2821 gagttcctaa tttaagaaag ttatcaaaag aaataagcca agggaattgg aaggaatttt
     2881 tagaagtagc agaggataca tactatgaag aagttaggct acaaggtatg gtaattggca
     2941 atataaatag taattttgaa gaaactttat actatgtaaa aagatttata cctaaaatcg
     3001 ataattggtc agtttgcgat ggattttgtt ctgatttaaa gtctatgaaa aaatataaag
     3061 aaaatatgta tgatatactt aaaaaatatg tttattcaaa gaatccatgg gaaattagat
     3121 ttgcattagt tatgttttta atatattatg ttgatgataa acatattaaa gaaatatttg
     3181 aatactgtaa taatatacaa agtgaagaat attatgttaa aatgggtatg gcatggttgc
     3241 tatcaatttg ttttattaaa tgtgaagagg aaacttttct ctatataaaa aataataatt
     3301 tagatgattt cacttataat aaaatgctac aaaaaataat tgaatcaaat agagtagatt
     3361 tagaaaagaa gaatataata agaagtatga aaagaaagac aagaagatgt tgacggagga
     3421 atattaatga ataacaatga agacagaatt gacaaatttt tagaacttca aagcaaagga
     3481 tttaaatttg cagagatagc tcaaaatcta aagattagtc aaagtacttt aagaactttt
     3541 ttaaataaaa aaggatataa gtcagaaaaa ggtaaataca tatttaaaga cgaattaaat
     3601 tataataaaa gtttggataa tagaaacttg gaagaagaga aagctaagga gtatgaaaaa
     3661 agtaaaaaga atactataag taatagaaaa aaagtaagca ccaaaaaaaa gatttctaat
     3721 gaaaaggctt taagtaaaag tgttgaacaa attgagttta atgatacagg tttaaaaaat
     3781 gcaaagccaa aacaaaatat taagcctaag aaagatagaa aaataaatat aactcaagaa
     3841 gatttagata agctttgtga agtatatgat tggtatttag aagtaaaaga ttacaatata
     3901 aagcctaaaa aagtatctaa taaaaaagat gtacatatag atgatgaaca catagatgaa
     3961 ttaaaaacaa caagaatacg tatagataaa gaaatatgga atgattttga aagattatgt
     4021 agtaattcga attttgaaaa acatcaaata cttacacagg cattgaaaga atttatgaaa
     4081 aagtataaaa atctactata aaatactact atatgaggtg cttaatatga ataaaatagt
     4141 aaaaaaatca atttctctaa tgattatatt aacaatattt atttttatgt tgacagcatg
     4201 tgaaaaagat gaacagcctg attcagttga tattcaaaca gaagataaaa atgagataaa
     4261 aatagatgag ggaaatgcaa aagtattgaa tataggaaaa tcagaaatta taaatataga
     4321 tgaaggtgat aaagtagata catcaacaaa ggtagaaaat aattcaacat ttaatatatc
     4381 caatgttgaa ttgatatata atgaatatga tgagaataaa aaaattatat cttcggatag
     4441 taaggctttg ttggacatga ctttaatgcc tgggaaagtt gcttacatag aatgtgggca
     4501 taaaactttt gtaaagggtg tagaagttta tgcatatgaa tatgaggcag agggtaagat
     4561 tgtgtatgtt aatttaaaag aaaatactat taatataaga aataataata ttaagctaga
     4621 aaatagtagt cagtatgagg tgctatctac atcagaactt aaaaaagtaa atgaatcaaa
     4681 tgagggaatt acgtatcaag taaaagtaaa aaattcatca tcaaaagatt taggaaatat
     4741 tattcttaag acagcagaag ttaatgaaaa tggagaatat ttaacagtaa atcgtgtgcc
     4801 atcatataag attttaaagc cttcagaaga gacagatata gatatattgt gttcaacaaa
     4861 ggcaaaaagt gttgaaatag tgggatatac gtatgatgat ataaaagaaa aagccaatgt
     4921 ggatattgac ttaaaatctc ataaagttaa gatagacaaa taaaggtagg ggatacttat
     4981 gaaggtttta atagttgaag ataataagat acttttagaa agtgtagtag aagaacttag
     5041 caagcatttt gaaactgaaa agtgtgaaga tggagaagaa gctttatact tagtaaatca
     5101 aaatatatat gatttagtca ttttagactt aatgcttcct aatataaatg gatttgatat
     5161 cctaaagaaa atgagagtta ataatataga tacacctgta cttatattga cagcaaaaga
     5221 aacattagat gacaaagtag aagcatttac tataggagct aatgattatt tgacaaaacc
     5281 tttttatatg gaagagttag tagctagggt atacgctata ttaaggacta atggaaagat
     5341 aaaagaaaga aacggattag aatttaaatc tttatattta gatacactag aaaaaagagt
     5401 gtatattgaa aaagaagaaa taaaacttca aaacaaacag tttaacttgc ttgagtattt
     5461 tgtattaaat aaaggttcaa ttttattaaa agagcagatt tatgatagaa tatggggaat
     5521 tgattcagat gcgactattg aaatagtgga agtatatgta agtaatctaa gaaaaaaact
     5581 aagtaaatat ggatatgata aatatattaa aactaaacgt aaggtaggat atatattcga
     5641 tgataaataa gaatgtattt accagtacta aaaatcattt aataaaaatg tatattattg
     5701 tagtaggtag ttttttaata atcttttcta tatttatata ctcatatttt aggggactta
     5761 catatagtgg aattgatagt gagataaatg atgaacttga atatatagtc tctcaattta
     5821 aaagaacttc atttttgaac ccaataagac tgaaagatcc taaagatatg gtgtatgtgt
     5881 atgaagatgg aaggataagt tactatactc aaaatgaata ttttgatgag ttacttccag
     5941 atagaagact agataaaaaa aattcgtttt ttaaatacac agaaaatgga tatactttta
     6001 gagaattaaa tgtagatgta ggtagatacc aaatacagat aattagaaat atagattctg
     6061 agatgaattc tttaagacag cttacatctg ttcttataat tggaatatta atttctgtaa
     6121 taattactta ctttgtggct gtatatctca ctagaaaagc tttaattcca atagaaacag
     6181 catggaaaaa tcaagctaag tttatacaag atgcttctca tgaattaaga acaccaataa
     6241 caatagtttc atcaaaatta gaaagtatgt taaaatcccc agaaagtaca gtaaatgatg
     6301 aagttgaaac aatagctact gcaatgaagg aaactagaag acttaaaaaa atgataactg
     6361 atttattgtc tcttactaaa gaggactcta ttgttaaagt taatttagaa gaaatagatt
     6421 tagaaaagtt attggaagaa atatcagaag actatattga cattgctgag tttcaggaaa
     6481 aaagattcgt gtttaattct aaacttaaga ataaagttat aataacagat aaaaataaat
     6541 taagacagct tatattaatt ttcattgata atgcctttaa gtatacaaaa ttgggtgatg
     6601 agatagcatt agaattgaaa gaagatatag aggatgaggt aactttactt ataagtgaca
     6661 ctggtatagg cataaaaaaa gaagagatac ctcttatatt tgatagattt tttagaagtg
     6721 aaaatgtaag aaataaagat ttagaaggtt ctggaatagg actttcaata gctcgcatga
     6781 ttagtttaaa cttatctatt gatataaatg taacttctga tgtagatatt ggaactacat
     6841 ttgaattgtc aatacccaaa aagttaaaat aataaaaata taaaaaattg tttttagaaa
     6901 tgaagaatgg gtatactaat tagtataaaa tataaaaaga ccttataaaa gtaaatttat
     6961 atttataaat ttcatagtat aattatatag ggataataaa gtttaatagg aggatttaaa
     7021 aatggcaaaa gttataaata catctgaatt tagaagtagt gttgaaggta gtaagggagt
     7081 agttgtagtt gatttctttg caacttggtg tggaccttgt aacatgttgg gacctgtatt
     7141 tgcagaatta ggtgaagaga tgaaggataa agctagattt gtaaaagttg atatagatga
     7201 gagcttagaa atagctcaac aattcaatgt atctacagta ccaactatga ttatatttaa
     7261 agatggaaaa ccagtagaaa ctttaattgg attcatgcca aagaacaaaa ttgaaatgca
     7321 agtaaaatca tatttataaa aaatgggtga gtattatgag atatgatata gctataatcg
     7381 gcagtggtcc agctggattg tctgctgcta taaatgcaaa aataagaaat aaaacaatta
     7441 tcatgtttgg taatgataat ttaagtaata agcttgttaa agctccttca atagataatt
     7501 atttaggttt ttatgatata agtggtgatg aattaaaaga caagtttaaa tcacatatag
     7561 atagtatgga tatttcaatt gaaaataaaa gaataaataa tatttatgct atgggagaat
     7621 actttacaat aatgtctgga aatgatatgt atgaagctac tactgtaatc cttgcaactg
     7681 gggtagagta tactagacca atcaagggtg aagaagaatt tctaggaaga ggtgtgggct
     7741 actgtgctac atgtgatgca ccactttata gaaataaaaa ggtagcagtt ataggatata
     7801 atgaagaatc taaagaagaa gcaaattttt taagtgaact cacttcaaag acttatttta
     7861 tacctatgta caaaaaagat aatcttatga gaagttcaga taacctagat gattctattg
     7921 aagttataca tgacagacca gtacagatag atggagataa acttgtaaac aaagtatctt
     7981 ttaaagaaaa ccacattgaa gttgatggag tattcgttat aaaagatagt acagctccaa
     8041 gtgcattggt tcctggaata gagattgatg gcattcatat taaagttgac aataatatga
     8101 agactagtat agatggatgc tttgctgctg gagattgtgt tggaaaacca tattcttata
     8161 taaaagcagc tggtcaaggg caaatagcag ctttaaatgc tgtttactat ttagataaat
     8221 taaagagggc ttaatattta tattgattaa ctttttaaag atatttataa aaaactgtct
     8281 tgaaataatt ttattatttc aagacagttt tttattttta agttattaat aaaagtattt
     8341 taaaatctat atgtaattac ttttattaat aaaactcaaa gttatattag taaaaattgg
     8401 aattatttat aatatactcc aaaatttaaa aataatggtt gaaaaatcta attaagagta
     8461 ctataattta attaagtaat tacttaatca aaacgaggta cttatggaaa acttggttaa
     8521 aatatttaaa gcattgagtg atgaaactag acttaacata cttatattag tatccaagag
     8581 aaatatatgt caaaagggga tttctaaata tctagggatt tcagattctg ctgtttcaca
     8641 acatataaaa atattaaaag atgttgggat aataacagga tataaagaag gatactatgt
     8701 tttatatcat attaataaag aatcatttaa tatatgtgta gattttataa attctatgtt
     8761 aagtaattct agtgaaagct ttatagatgt atttgatgta aatacaatac atctaggatg
     8821 cagtaaagaa tgtaaatcta taaaaaaatg ttgtaagaga agggagaaat aataatgaaa
     8881 gtatgtatac cagtagagga gaataaagga ttggatagta aaccatatgg acattttggt
     8941 tctgctccaa tttttgttgt gtgtgattta gaaagtggag aagttaaatc tttagataat
     9001 ggagatttag atcatgaaca tggaaaatgt caaccattaa aagcattgtc agggacagct
     9061 gtagatgcag tagttgttgg aggaatagga caaggagcta taattaaatt aaatggaatg
     9121 ggaataaaag tttacagagc agaaggtgat actataagtg ctaatttaga cttacttaaa
     9181 aatggaaaat tagtagaatt tccatcagac catacttgct cacatgacgg atgtggacat
     9241 cattaaaaaa aaacagctga tgaaatatat acttcatcag ctattttttc ttttaataaa
     9301 tacgatttta ttcttttaaa accaaaattt agatttttat attttttgta gtatccttaa
     9361 taatatttca agtttacaag tttactactt ttagattttt tttgtatgtt tttctatata
     9421 gtcaatatca tttaatatgt cattgagttc tttattaagt gtttttaaat catcaccatc
     9481 tacattgtag tttaaaaata gttcctctac attatcaatg atagaattga cttttctagt
     9541 aacttcaaca aatgtcttaa agttactact atttttagca tttagaaatt tttttaaatc
     9601 ctttgatata aatgatacta tttcatattc atcattaaat agacctctac caataggata
     9661 tggttcaatt ctctgtaaaa aatattggtc tttagcatta atattataag tataagatat
     9721 ttcccaaccg tctcctaaat caaacttgat aaaatataat gtactactta cttttgatat
     9781 tcttgtggca ccaagctgtc ttaaaagaac ttcaatcgaa tcaccttgaa cagtattaat
     9841 attcatatat acacatcccc tttagtatta ttatactata ttttttaaaa ttttataatt
     9901 aaataaacag tagatataat aatagatata atcataagtg gaaaacctat tttgaaatac
     9961 tccttaaaag agataggatg accgtgttta ttacctatac ctgctagtac aacattagca
    10021 gatgctccta ttagtgttcc atttcctcca agacaagctc caagagatgt tgcccaccat
    10081 agtggcatta catcaatacc ttcagcttgc atagtcaaaa taagaggaat tagtgttgca
    10141 acaaatggaa tattatctaa gaaagaagag actatagctg acaaccataa tatcaacaac
    10201 attgtaaata caagatgtcc ttctgtaagt ccaatcaatg cttgagccaa tttgtttatt
    10261 atcccaactt ctactaatcc accaactact acaaataatc ccataaagaa taaaattgta
    10321 gaccattcaa tacctgccat aatttcatct acatcttgtt ttcctattaa aagcattata
    10381 gatgctcctg tcagtgctac aacagaagaa tctatgtgga ttgtactgtg aaacataaaa
    10441 ccaaaaagta tgaatgctat taatataagg ctttttataa gtagaggttt atctttaaca
    10501 gatttttttt catctagttt taatattgca tttttagctc tttcattcac aacaagttct
    10561 tttccataaa taaatctaaa gcatattata gttatagcaa gtattactat aacagcagga
    10621 cctaaattta ttacaaaatc cataaaacta agattggcag cactaccaat cataatattt
    10681 ggagggtctc ctataagagt agctgttcct cctatatttg aagctaaaat ctgtgttatt
    10741 aaaaatggaa ctggattaag tcctaatatt tgtgtaataa ctatagtcat tggtccaata
    10801 agtaagacgg ttgtaacatt atctaataca gctgataaaa tagctgttat aatagcaaag
    10861 caaatcatta tcttccatgg gtcaccctta gctttctttg cagtccagat agctatatat
    10921 tcaaataacc ccgagttttt gacaacagct acaaatagca tcataccaac taatacacca
    10981 atagtattga aatctatatg gcttaatcct tcatcaagtg taagtatatt gaatattatc
    11041 attaatagtg caccaccaac agctgctacg gatctgttta ccttttctgt aataatagct
    11101 gccataacta ttaaaaatat acatatagct attatttgtt gaaattccat aatataagta
    11161 cctccatttg tattattttg tgcctaatgc tagtactata tatagtatta cttttttatt
    11221 gttttttcaa tggatgtatg aaagtatatt atttaacaaa gtttttgaaa aattgacaga
    11281 atttaaagaa aatttttgtt atttaaataa attttaaaat tattccattg ataataatat
    11341 taaacgacaa acgaaaaaat atacccttta aactagaatt tattgtatct aataacaatt
    11401 ataaaatatt aaatatatct atattataaa aacaataaaa aaaggtttga tttgaata
//
LOCUS       DBGJFU010000119        11412 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.119, whole genome shotgun sequence.
ACCESSION   DBGJFU010000119 DBGJFU010000000
VERSION     DBGJFU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.119"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            119..1561
                     /locus_tag="ACOIAS_002998"
     CDS             119..1561
                     /locus_tag="ACOIAS_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2702692.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1603..1956
                     /locus_tag="ACOIAS_002999"
     CDS             1603..1956
                     /locus_tag="ACOIAS_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702693.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2147..2968
                     /locus_tag="ACOIAS_003000"
     CDS             2147..2968
                     /locus_tag="ACOIAS_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HGZ2702694.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
     gene            3226..5109
                     /locus_tag="ACOIAS_003001"
     CDS             3226..5109
                     /locus_tag="ACOIAS_003001"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HGZ2702695.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            5334..6014
                     /gene="pgmB"
                     /locus_tag="ACOIAS_003002"
     CDS             5334..6014
                     /gene="pgmB"
                     /locus_tag="ACOIAS_003002"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGZ2702696.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            6011..8296
                     /locus_tag="ACOIAS_003003"
     CDS             6011..8296
                     /locus_tag="ACOIAS_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HGZ2702697.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
     gene            8521..9414
                     /locus_tag="ACOIAS_003004"
     CDS             8521..9414
                     /locus_tag="ACOIAS_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGZ2702698.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            9851..10342
                     /locus_tag="ACOIAS_003005"
     CDS             9851..10342
                     /locus_tag="ACOIAS_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702699.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            10370..11317
                     /locus_tag="ACOIAS_003006"
     CDS             10370..11317
                     /locus_tag="ACOIAS_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702700.1"
                     /translation="MRITKTTFNDVFSSDANESIIKVEKYVDKKCASVFEILTYTIIV
                     TNTNRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         4711 a         1040 c         1953 g         3708 t
ORIGIN      
        1 atttatgata taataacaaa tatataatta ttaaattata ataaatgact atagcgtaga
       61 tgtaatacta tgttatagtc atttttgaat taaacttgat atatcaaggg ggaagaatat
      121 gagaacaatt agaagagttt tagcattagg tttaacatta gctatatttc taataaatgt
      181 acctaatgta gatgcattaa catcagatac aattaaaggt aacaatatat atgagacggc
      241 tggattaata gcagataaaa agagttatga tacagcaatt atggtaaata tggataattc
      301 tatagcagat ggtctttcag caagtggtct tgcaggtgct gttgatgctc caattttgct
      361 tgcacaaaaa aataagatac caaatgaaac aaaacaaaga ttaaaaaatg ttaaaaagat
      421 atacataata ggtaaagaat tatcaataag taagtcagta gaaactgaac taaaaaacac
      481 aggggcacag gtaactagat tgggtggaga tgacagaata aagactagtt atagtgttgc
      541 taaagaggta aatggtatca aaaaagttga tgaagtgata ttaactaatg catataaagg
      601 agaagcagat acaataagtg ctgcacctgt atcagtaaga gatatagctc ctatagtact
      661 tacagatgga aagagtgtac ctttttcaac aagcaatgta aaaacatatg cagtaggtgg
      721 gagtatatca atgagcacta gtttagttaa taaaactaat gctaaaagac ttggtggttc
      781 agatagatat gatacaaata agaaagttat aaaagagttt tatccagatg catcagaatt
      841 ttatttgagt gatggatatg atttagtaaa tgcacttaca ggttctacaa ttgctaagga
      901 aaatccaatt gtattggtgt cagaaagtag tgataagtct atattagcag gagcagataa
      961 gattactaga ttaggttcaa taagtgacag tgtgtataat aagtgtgttt ctgctgcaca
     1021 aaataatggc gattcgtcta caaaaggtga gtcacctatg aaaaatgaga caagcatatt
     1081 aggtcagcca acagctagtt tagaagcgtg cttaaaatgg gcaaaatcta aaaaagctaa
     1141 tgatttattt atagagttaa taccaatatt atatgatact gctgttcagg aaggtgttaa
     1201 tcctgttttg gcagtagctc aatctgcaaa agaaactggt ttttgtaatt ttggtggagt
     1261 attagatgca tcatttaaga atccttgtgg acttaaaact tctgtaggag gttctgatac
     1321 tgataagaat gcccattcaa gatttgatac ttgggaagaa ggaatattag ctcaaattca
     1381 acatttatgc ttatatgcag gacaagatgg ttacccactt tcaaatccag tagaccctag
     1441 acatgaaaaa tctttatttg gtaaggctaa aacagttgaa agtttatcta ataattgggc
     1501 tggaggtcaa tatggacaag atttagtaag aatgatggga gaaattgagg caacaaaata
     1561 attgaagatt atcaaatatc caataaataa ggattgatta ttatgaaaaa gagtaatatt
     1621 tttagtatca tatttcctac agtcataatg gtgctaatgt ttttaacttt tgctacagat
     1681 atatttaata ttccagatat tcaatctaaa agtatttttg ttgtgggaat ggttttgatt
     1741 tttccaattt cttttttgat acaagggata atttgtgtat taaataagac taattggatt
     1801 ttatctctta ttgtgtcttt agtaacttat atagtattaa tgtatatatt tttaaatgat
     1861 tctgcatata tatatgtttt actttataca gctttttata tgattggaca tataacagct
     1921 aaactctatt acaaaattaa gacttttaaa aattaaattg aagtatataa aagtaacatt
     1981 aggagaagta tccttaatga ttttctaaaa tcagaaatag gtgcttctct tttttgtaaa
     2041 aataaaagta tgtaaataaa atttaaccat tataattttc ttttaacaaa tgtatacaat
     2101 aaacttgttg tataattaag atagataaaa taaagggagg gataatatga atttaataga
     2161 taatcatatt catactaatt tttcaagtga tggaaaggat tccatggaag atacaataaa
     2221 aaaagctata agtattggtg ttagatattt gacttttaca gaccatttag aacatgatga
     2281 agatagagga ttttgcataa attataatga ctatgttcca gtatttaata agtttaaaga
     2341 aaaatataaa aaggacatag agttattact aggtgttgaa gtgggttaca gaaaacatct
     2401 aaaaaatgaa atagaagaaa taataaattc taatcctttt gattttgtat tgtgctcaac
     2461 tcatacaatt gacaatgtac ctgttccatc aaaggagtat tttaaaggtc ttagcaaaga
     2521 agatgcgtat tataaatatt ttaatagcat acttgaaact aatcgtgaat ttggagatta
     2581 caatatatat ggtcatttgg attatatatc taggtatgga atatattcag ataatagagt
     2641 tatatacaat gactttaaag atgttattga tgaggtctta aagtcaataa tcaataatgg
     2701 gagtggtata gagctaaata cttctggtta tagatatgga ttaaatgcta tacatccaaa
     2761 tgaagatatt ctaaaaagat ataaagaact tggaggattt atagttactg taggttctga
     2821 ttctcatagg gtagaagata tatgtaaaga ttttgatgtg gcctatgata tgcttaagta
     2881 tcttgacttt aaatatgtat ctttatttaa agaaagagaa acttattttg taaatataga
     2941 aaaagttaaa tctaacaata tagcttagag tgtgttttga agtaaattaa tggatagtta
     3001 aaaagttaat agttataata ttgtatcctc tagaaaaata ctagaggttt ttattttgct
     3061 aaaataacta aatagaatta aatttaaata gtatagttta ttttaaattg aatagtttat
     3121 tgttgatata ttttactatt atatattttt attgtgatat aatagtaaaa tatattaacc
     3181 tatctttaga ttatataaat tattataaag gagggaaatt ttaaaatgtc taataaaaat
     3241 aaagaaaata taactttaag taatcaaatc aaaggaacaa ttcaaaatag tttaacaaaa
     3301 gaagctatat tacatatacc aatgagcaat tatgcctatg gttatgatag agaaacacta
     3361 catataagac ttagaactaa aaaaaatgaa gcgaaaagag taattttacg aataggagac
     3421 caatatgtgt gggataaagg aggtgcagga ggaggaaatt taaatgcatc tggagttggt
     3481 tggtctggtg gaacaaatat agtaatgagt aaagaagtag aaactgaatt atttgactat
     3541 tggatagcta agtgtaagcc tttaaataaa cgctcacgat atggctttat aatagaaggt
     3601 caagaggaaa aacttttatt tacagaaaaa agaataatag agttgggaaa tgaaaatgat
     3661 gaaaaggaat tatgtgaaat aggtaatttt tttggatatc catatttaaa ttatatagat
     3721 atacctaaag tgccaaattg ggtgaaagat actgtttggt atcagatatt tccagataga
     3781 tttgcaaatg gtgacccgtc tataaaccca gaaggagtag ataaatgggg agctattcca
     3841 accagagata attttacagg tggagattta caaggtgtga tagaacactt agattatttg
     3901 agtgatttgg gaataaacgg aatttacttt tgccctatta caattggaaa aaccaatcat
     3961 agatatgata cggtagatta tatggaggta gaccctacat taggagataa agagacttta
     4021 aaaaaactaa tagaagaagc gcataagaga aatataaaaa taatgttgga tgctgttttt
     4081 aatcatatag gatattattc taaacagtgg caagatgtag ttcaaaataa ggaaaattct
     4141 cgatataaaa actggtttta tataaaagat atgagtaaaa ttgatactcc aatagaacaa
     4201 atagatgaaa aaaatatacc ttatgaaacc tttgggtgtg aaaaatatat gcctaaactt
     4261 aatacagaaa attcagaagt aataaaatat ttattggatg taggcaagta ctggatacag
     4321 gaatttgata tagatgcgtg gagattagat gtttcaaatg aagtagacca tgttttttgg
     4381 agaaagttta gagaagaagt caaaaaagta aaaccagaca tttatatatt gggagaaatt
     4441 tggcatggta gtttaccatg gctaatgggt gaccaatttg attcagttat gaattacttg
     4501 atgtcagaag ccatgaaaaa attcttttgt acagatgaga taaacgccga agaatttaag
     4561 tatatgataa atgatgtaat ggtaagttat ccaatacaag ttagtgaagt tatttttaat
     4621 ttacttggaa gtcacgatac tacaagaata ttgacttatg ctaatggaaa tattgataga
     4681 tttaaacttg catatatgtt tatgtttgta caaacaggat gtccttgtat atactatggt
     4741 gatgaaatcg gaatggaagg agaattaagt tcaatatctg aaggtcaaag aaaatgtatg
     4801 gaatggaatg aagaaaaatg gaataaggat atatttgatt ttatgaagaa gattattaga
     4861 cttcgtaaag aaaataaaga acttaggtct atatcaaatg aatgggtgtt agcagataag
     4921 gaaaatggaa cgattatatt gaaaaaagaa tctatttcta taataataaa taattcatct
     4981 aaaaatataa ttttgacgtt accagattat ttagcaaata aaaaagtaaa agatttatat
     5041 gaagaagaaa tagttgattt aaaagaagaa ctaatgttag agaaatacaa gttcattatt
     5101 ttaaaataat acaaaaaagt atttatttta gatttcatgt taaattatta tacagtttaa
     5161 ataagtcttt ataaacgata aaaaaatatg gtctacaagt gagtgaattg aaagtagatt
     5221 ttaaattaaa gactgagtta gagtagataa ttttataaat aatatcttaa atacaagata
     5281 gaaaataaat tgattattag tctaaaaatt tcttgtagaa agaggaaata agaatgattg
     5341 aagcatttat atttgattta gatggtgtaa ttacagatac tgcatattat cactatatgg
     5401 cttggagaaa actagcccat aaagttggga tagatataga cacaaaattt aatgaatctt
     5461 taaaaggcat aagtagaatg gagtcattgg acaggatatt agaatttgga aataagaaat
     5521 attcattttc agaagaagaa aaagtgagaa tggctgaaga aaaaaataat tactatgtat
     5581 cattgataga tgagataact tcaaatgata ttttgccagg tatagaaagc ttgttaattg
     5641 atgtaaaatc taataatata aagataggtc tatcttctgc aagtaagaat gcaataaatg
     5701 tattaaatca tttgggtata agtgacaaat ttgattttat agctgatgca ggaaaatgta
     5761 aaaacaataa accacatcca gagatatttc ttatgtcagc gaaagggctt aatgtaaatc
     5821 cacaaaattg tatagggata gaggatgcaa gtgcaggtat agacgctata aactcagcta
     5881 atatgttttc tgttggtgtt ggaaattatg aaaacttaaa aaaagcaaat ttagttgtag
     5941 attcaacaaa tcaattaaag tttgaatata tacaagaaaa gtataatgag tatatagtta
     6001 ggagaattat atgaaatgtt tatttagtaa tgtagatgag tggaagatta tacaagataa
     6061 gattgaaatc aaagaaaata gattagcaga gtccataatg agtataggca atggatatat
     6121 gggtatgaga ggaaactatg aggaaaatta tagtggagat acacacaagg ggtcttatat
     6181 agctggagta tggtttccag ataaaactcg tgttggttgg tggaaaaatg ggtatccaga
     6241 atactttggt aaaattccta attcagtcaa ctatataggt gtaagaattt tcatagatga
     6301 tgtagaatta gatttagcaa aatgtaaagt agaagatttt tatagagaat tagatatgaa
     6361 aaatggtatt ttaaatcgta ggtttattgt aaatataaat gataaaaaat ttgaagttag
     6421 ggttgctagg tttttaagta tatctgttaa ggaacttgca gtaattaaat acagtataaa
     6481 atcattaaat tctaatacta aaataaaatt tgtaccatat ttagattcaa atataaaaaa
     6541 tgaagattca aactatgagg aaaagttttg gaatggaatt tcttcaagtt ctttagaata
     6601 taaaggtagt attctttctc gaacaaagga gaatgcattt aatacaccag tattcactgt
     6661 gggttcaatg atgtatatta agacaaatgg tgatgtagaa gctgtatctc atgagagtga
     6721 ttataattac tgtgaaaatt cgataacaat ggatgtttta aaaaatgagg ttgcaagtat
     6781 agaaaaatat gtatctataa cttcaactag agattataag gaaagtgagc tattaagaaa
     6841 atgtgaagac attttaaata gagaactatc taaaagttat gatgatatat taaatgaaca
     6901 tacaaagtta tggaataaaa gatgggaaag tgcagatgta aaaataaatg gagataaaag
     6961 tagccaacaa ggtataagat ataatttatt ccaactattt tcaacatatt atggagaaga
     7021 ttcgcgatta aatataggtc caaaaggatt tactggtgaa aagtatggag gagctactta
     7081 ttgggataca gaagcgtttt gtgttccagt atatctaggt gtagcagata aggaggtaac
     7141 tagaaatctt ttagaatata ggtacaatca gttagaacag gcaaaggata atgcaaaaaa
     7201 actagggctt aaaggtgctt tatatccaat ggtaacattt gatggagtag aatgtcataa
     7261 tgaatgggag ataacatttg aggaaatcca ccgaaatgga agtatagtat atgcaatata
     7321 taactataca aattatacag gtgactataa atatataaaa gaaaaaggaa tagatgtaat
     7381 aatagaaata gctagatttt gggcaagtag agttcatttg tctacaaaaa aagatttgta
     7441 tatgatacat ggagtaacag gtccaaacga gtatgaaaac aatatcaata ataattggta
     7501 tactaattat atagctaaat ggtgtttaga ttatgctgta gaaaatatct tacgattaga
     7561 gaaagagtgt aatgaatgta ttgacagaaa taatgtgact agggaagaag ttgagttttg
     7621 gaaaataata tcaaataaaa tgtatctgcc gtatgatgag gaactacaaa taataattca
     7681 aaatgataat tttttagata aagaatttgt agaagtaaag gatttaccaa aagaaaattt
     7741 tcctcttaat caaaattggt cttgggataa gattcttagg tcgtgtttta taaaacaagc
     7801 agatgtatta cagggaattt actattttgg aaatagattt actaaagaag aaaaaaagag
     7861 aaattttgac ttctatgaaa aatatactgt acatgaatct tctttatctt ctagtattta
     7921 ttctattatt gcctcagaaa tagataacct agaaaaggct tatgaattat attcaagaac
     7981 agctaggctt gatttagata attacaataa tgatacaaat gatggactgc acataacttc
     8041 aatgtctggt tcatggcttt ccattgtaca tggatttgca ggcatgagaa catggaatga
     8101 aactctatct tttgacccta agttgccaaa agagtggaaa aattattctt tcaatataaa
     8161 ttatagagat aatattatta atgtagatgt atgtagtgat ttaattaaaa tagaaaattt
     8221 aaaaggtaga ccaataaaaa ttattgtata tgggaaaaaa tatatattag aaaaatgtat
     8281 aaaaattgat aaatagattt aataatattt ttattatata aatagttaaa ataagctatc
     8341 taattattta tttattttaa ctattttgtg tgatagaaaa ctatatatta taacaatagt
     8401 agtgctttta attaaatata tttcataaat cttcataaaa tcttcaaaat ttcgtaaaga
     8461 atatgtcaca aaagtaaggt atattatatt cagcagatag tataagggag gacaatattt
     8521 atgaagaata aaaaattcat tgttataata ctgataatat caattttaat aggtgtatta
     8581 gtgaaggaat attcatctag agagataaaa aaggatgata taaatgtcag taagtatata
     8641 aaatatgctg atttagcaag taaaaataat gcacaagtta actggaagta tgtagcttcc
     8701 attgttgctg tgttaaataa aaataactta aaaaacgtta aggattcaca gatacaagaa
     8761 gtatcagatt tatttgttaa gaatttttct aaaaataata aaataaataa gttaagtgat
     8821 attttggatg agttagaatt tagtaataga caaaaaagat tggttgataa ttatatagac
     8881 aatcttaagg attatggtat aaaaccagaa agattgaagt ctgatacaaa atatatgaag
     8941 tttattgatg aaattaaaac agaagcaata caaaattata aggattataa aatacttcca
     9001 tctataacta ttgcacaagc tataatagaa tctagctggg gcaagtcaac tttggcaaaa
     9061 caatatagca atctatttgg tataaaggct gacgcttatt ggaaaggtaa gtcagtgact
     9121 ttagaaacga aagaacattt agatacaatt atagatgata aatttagaat atatgatgat
     9181 aaaaatgaat ctataaaaga ccatgcaaaa tttttagcca caaataaaag gtataaaaac
     9241 aatggtgtat ttgatgcaaa aacgtatata tatcaagcta aagcattaga aaaggcaggt
     9301 tatagtactg cgaaggatga aaatggcaat agtatatatg cagctcgtct aatagaatta
     9361 attcaacagt ataatcttca attaatagac agtgaaattc aaagtgaaat gtaactttga
     9421 ttaaattttt attgataagg ctatgatttt taaaatcata gccttatttt gacatgcttt
     9481 tacttgtaat tattaaatcg atatcattat ctttcaaaat ttactttata ctgtaaatca
     9541 aattatttaa gttaaaactt ggaattattt ataaaaggat tatttacttt atctaaattc
     9601 tgcacttaaa gaacatcttt tcccttaaga ctgaccgtta gtctataaaa attataatta
     9661 cttataaaaa agtgaatata tatataaagt tttcagaaag acttttactt gtaaattgaa
     9721 atcaatgaag taaaaaagga ttctttctta aaagtaaata acaactagat agtgaataat
     9781 aaaaaatggt aatttgaaat aatttttatg aatagtatta atatatagat acagggagat
     9841 tgatgcgatt atgtatgaaa ataataaggt aatatacaac cttgaaagat gtgaaaaact
     9901 agaaaagact ctttatttat ctcaatttaa tacatataaa aattttaaat tagcttctaa
     9961 aaaaactgta ataaattcat tagagacata tgtttacaat atgaatatta acaatataag
    10021 tcttgttgat actatagaat gtatatcatt agaagatgaa caacttacag gaaagaagtt
    10081 gattttagtt ggtgatatag atattgatgc aatattagat tatgcaggaa ataaaagaaa
    10141 tagaaattct agtaaaaaaa atacatttaa attaaaaata ccctttagta catttataca
    10201 aatgcctaga aagatagaaa ataaagataa aattaattta aagtatctta tacaagatat
    10261 tacttcatct attttagatg ataatttatt tattagtgtt actgcaatta taagttatga
    10321 aaatagttcc aagatagagt aatctttaaa ttatagaaag gtggatgcaa tgagaataac
    10381 gaaaactact tttaatgatg tattttcaag tgatgcaaat gaaagtatta taaaagtaga
    10441 aaaatatgtt gataaaaaat gtgcatctgt atttgagatt ttgacctaca ctattattgt
    10501 tacaaataca aataggtaca aaactggaaa tatatttttc aaagattata tttctaaata
    10561 tatagagttt ataaataata ctgtaaaagt taatggcatt ataaaacgtg gattagaccc
    10621 acagcaaggt ttttacatag ggagaataga tgcaagttgt aaaaaaataa tatcttttaa
    10681 gagtgtagtt ttaccaaatt ctgcacatag gattataaag aatagtgcta atatatacta
    10741 ttattataag tgtaatctag ataaattccc aacaagaata tcaatagaaa gtaatagagt
    10801 ttatactaat gtaaataaaa ttgtattcaa acaattgaat ataagtaata ctttaaaagc
    10861 tcctaaaaat ctaagagata tattaaaagt aaatgtaaat agtaaaatat ttgatgttaa
    10921 acctatgaaa aatcttataa acaaaggtag taatttgaaa ttatgtaact tgatagtttt
    10981 tgggtgtata gaagttgaaa tagactatag ttgtaaaaat aggagtaatt ttaaaacagt
    11041 aaataagtct aatgacatta aaaaagataa gtattataaa aatgatatac aaaataacaa
    11101 tgaaaatata aacgattgta aaaagcaaat aagttttggt gataataagg taaaaaaaat
    11161 aacaaaaact tttggatttt cttgtttcat ttgttctcca gttggaatta tttatgaaga
    11221 tatgaaaaat ataaatataa aaattgaaca tacatcaata aatgaattaa atccagaaga
    11281 attatttgta aatacatcac ttttattgta ttattaagta ttttaaagct aaaacttaaa
    11341 cttattaaaa tagcatttgg ttaaaggaaa atagatattg atatatttat catatatata
    11401 aatgattaat ta
//
LOCUS       DBGJFU010000120        11387 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.120, whole genome shotgun sequence.
ACCESSION   DBGJFU010000120 DBGJFU010000000
VERSION     DBGJFU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.120"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            155..1330
                     /locus_tag="ACOIAS_003007"
     CDS             155..1330
                     /locus_tag="ACOIAS_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2702701.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1503..2900)
                     /gene="asnS"
                     /locus_tag="ACOIAS_003008"
     CDS             complement(1503..2900)
                     /gene="asnS"
                     /locus_tag="ACOIAS_003008"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGZ2702702.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(2977..3210)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3379..3573)
                     /locus_tag="ACOIAS_003009"
     CDS             complement(3379..3573)
                     /locus_tag="ACOIAS_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HGZ2702703.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3846..5519)
                     /gene="cspC"
                     /locus_tag="ACOIAS_003010"
     CDS             complement(3846..5519)
                     /gene="cspC"
                     /locus_tag="ACOIAS_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid germinant receptor pseudoprotease
                     CspC"
                     /protein_id="HGZ2702704.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5529..8927)
                     /gene="cspBA"
                     /locus_tag="ACOIAS_003011"
     CDS             complement(5529..8927)
                     /gene="cspBA"
                     /locus_tag="ACOIAS_003011"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional germination protease/germinant
                     receptor pseudoprotease CspBA"
                     /protein_id="HGZ2702705.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9094..10290)
                     /locus_tag="ACOIAS_003012"
     CDS             complement(9094..10290)
                     /locus_tag="ACOIAS_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGZ2702706.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10311..11132)
                     /locus_tag="ACOIAS_003013"
     CDS             complement(10311..11132)
                     /locus_tag="ACOIAS_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2702707.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3645 a         2075 c         1314 g         4353 t
ORIGIN      
        1 tactcgacat tatctaataa attttatcta tcacaatttt agattgttac acacaatata
       61 ttttgatata attaaatcat aaacgaagta ttccaaatat ctaatgatag aaaggaatac
      121 ttatttttat gctttatttt taatggaggc ttatatgaac ttagaaaaaa taaaaaaatt
      181 ttttggattt cctctagaca aaaaacacaa agacaatgtt gagaaagaac tctgcaatta
      241 tctttatcat acgggtaaaa tcatttgcat aataatagta atatctcaaa taattatgat
      301 gatatcaatc tcagctagag aaggaggccc ttttgtcaca ccaagacgcc aatggtattt
      361 ttatatgtat gtaacactat tttgtgtatc ttctgtcttt atgtttgtat tttcttacct
      421 ttggaataaa aaaaaggatt gctgtacgtt ttatataaat ttatggataa tttatgtgac
      481 ttttttctgc ctatggggtt gtgtaattac tctacttgac cagttaggtg gaaatgacct
      541 aagtgtattt agttatatta tgttagcagc tgcagctttc tcagtattaa aatttggaga
      601 atcagtaata gtttttggag gatgcttttt gtttcttaat ttattcttac ctgggattca
      661 aactatacca ggcaatgagt ttagtaatat tgtcaacaca ttttttataa catcccttgc
      721 catttttgtt tctacaatgc tatatagaaa tcgtgtattt acttactatg ataaactttt
      781 aattagtcaa caatatgaag aaatatctca tataaataat ttacttaacg acaaagttat
      841 gacagatgac cttactaaaa tgaacaaccg tagatacctt gaagatattc tacaaaaaaa
      901 attaatcagc catgcaaatc aagaacctat aactggaatg atgatagata tagattattt
      961 taaacaattt aatgataatt atggacatca aaagggagac tattgtctac aaaatattgc
     1021 caaagtaatt ttagatttta caagttctga aaatgaacat gctatacgat atggtggtga
     1081 ggaatttttt atatgtattt ttaactgtaa tgaggcagat gctaaactta aatcagaatg
     1141 tttacgaaaa attatagagt cctgcaattt tgagcgtgat gatgttcctt taaaatgtgt
     1201 aactgttagt attggagttt atacaaagaa aattggaaag acaataacat taaatgagtt
     1261 tataagtaat tgtgataagg ctctatatac tgcaaaagaa cagggacgta attgtgttgc
     1321 attttattaa aaggttacat actattacat aaacctataa gtattatatc gcaaaataaa
     1381 agtaatcaaa ttatatattt atagtacaat aaaaataact tgcatatctc taaaatagat
     1441 aaatatgcaa gtcattttag tgtttatact taaagttata taagttttat tatatataat
     1501 tattaaaatt cagcattttt aggagttctt gggaaaggta taacgtctct tatgttagac
     1561 atacctgtca tatacataat tattctttca aatccaagac caaatccaga gtgagtagca
     1621 gtaccaaact ttctaagttc taaataccac caataatctt cttctcttaa tcccatttca
     1681 ttaattctat ctaacaatac atcttctctt tcttctcttt gagaacctcc tacgatttct
     1741 ccaactccag gaactagtaa atccattgct ctaacagttt taccatcttc attcattctc
     1801 atatagaaag cttttatatc ttttggataa tctgtaacaa acactggaca attatatatt
     1861 tgctccgtta tatatctttc atgctcagtt tgaaggtcac atccccactc aacaggatac
     1921 tcaaattcat gcccagattc aattaataat tccactgctt tagtatacgt tattctaata
     1981 aactcagagt ttactatctt atttaacctt tctaacaatc ctttatctat aaaactatta
     2041 aaaaattcca tttcttcagg agcattttct aatacatagc ttataacata ttttatcata
     2101 tcttctgcaa gttccatgtt atcttcaagg tctgcaaaaa ctatttctgg ctctatcatc
     2161 caaaattctg aagcatgtct tcctgtataa gagttttcag ctctaaatgt aggtccaaaa
     2221 gtataaacgt ttctaaaaga aagtgccatt atctcggcat ttaattgacc actaactgtt
     2281 aaattagcct cttttccaaa gaaatcttga gtatagtcta ttttgccatc ttcagttttt
     2341 ggaatatcat ttaaatccat agttgttatt ctaaacatct ctcctgcacc ttcacagtca
     2401 ctacctgtta tgattggaga atgtgcatat acaaagtttc tttcttggaa gaatttgtgt
     2461 attgcatagg cagctattga tcttactcta aatacagctg aaaaagtatt acttcttggt
     2521 cttaaatgtg ctatcgttct taaatattca agcgtatgtc tcttcttttg tagtggatat
     2581 gaactatctg attccccttc tacaactatg ctttttgcat gtatttcgac tggttgttta
     2641 gccccttctg tagatactaa ctctccctct acaagtatag atgagcttat tgctaatttt
     2701 cctacctctt taaaattttc tactttctca tcaaatacta tctgcatatt tttaaagaag
     2761 cttccatcat ttagctctat aaatccaaaa tttttagata ctctagatgt tcttatccat
     2821 ccagctactt ttacagtctt ccctatgtac tgttcttttt ctctgtacaa ttcttttact
     2881 gttataaatt ccttttgcat tttaaatcct cctagtataa ttgttattaa tttcttttaa
     2941 atataatttc aataaaaaaa gcccttcatc cctatataaa gggacgaaag actattactc
     3001 tcgcggtacc accctagttg tcataatgac ctcttagtta agcaatgctt aaagcctata
     3061 acggtgcctt ccgtctaagt ctacttacta aataagcttt cgattagaaa ctccaagatg
     3121 ttcttcaata taaacctcgt attgagcttt caccatcctc aactcgctag aactacattt
     3181 tatatctact cttctcttca cagtatttat cttttattat ttctgctaat taacaaaatt
     3241 ataccaattt ataaattttt agtcaataaa tttatataat tgatactgat tgttatttaa
     3301 aatataataa ttctacctta cataatgata ataaatactt atcttcatta cttctttatt
     3361 acttcaattt ccaaaatttt ataatttact tccattgtca acccagtctt ttgcttcttc
     3421 gacaccctta attgaaggta ttgaaacttt actatgaggt tttaattttt caacatcagc
     3481 ccatgctgct gtcgactcat ttccaactaa aagtgaactt ttaagtcttt tattatcttt
     3541 accttttaat gctcttgcct ttttactttc caaaactatc acctctatta tttattttgt
     3601 aatagtttta gcaattttac aaataaaatt cataagtcta aattataatt atttttaaat
     3661 taacatcata tcttttcaat acttaattta tacctattta tatacaaaat atttactttg
     3721 tgtattcaaa atataaaact gttttttaat tttcatattt attttttatg ctatttttct
     3781 actctatcta aagtatttga tacaattaaa ctaataatca attataattt tacataggtt
     3841 cttatctata gagtatttgc tatctgttga atcgtatttt ttaaatttaa aatcccatat
     3901 ccctgcgata catttggata tgtatatatc tcaagttttg tagcacccaa tattaaatat
     3961 gtttttaaaa cttgtgtaaa caatgataat ctcggtacat tatcctgttt ttctaagtat
     4021 tccatcaata aggctaaaac acctgtgact atagaactgc tcactcctgt tcctgttcct
     4081 gtattatatg ttccattttt atatgtggat attatatcta cacctgaagc tactatatct
     4141 ggcttaattc ctcttcctct aattggtcct ttcgaagaac caatccacat actatcagtc
     4201 ttattgttaa atgtaccaac tgtaattaca tcatcactag cagcatacat agttatagta
     4261 gctactgaat cagggtctaa aaaccttgtg tctggagcta tcaaattttt atttggcaag
     4321 tatatgtcat attctccatt tattataagt tctgaagtca atcttaaaat ccatactcca
     4381 ggttttatat ctcttaatct tatctccaaa ttttcttttc cagaggtaat atatggataa
     4441 ataaatctca ttgcataagt agtattttct aagttaaatt ttcctctata tatataaaag
     4501 tctggagaat accttatatc atgactaact tcacctgaag gagatattat ctgtgctccc
     4561 actttatctg gaccattcgt atttagtgtt atatcaagag catagtcatc tccatcttgt
     4621 attattacat cttgaacttc tcccacactg ctaaatctgc cagaataatg aatatctgta
     4681 ttcccttgat ttcctgcacc actcactact accacaccag cactactcaa tatattaaaa
     4741 gtatctagta ttgaagtagt tgctacagca cttgacttaa ctccaatagt caaatttatt
     4801 atcagtggtt tattttctgt tcttgctatg ttagtaacat acgttatagc tgctaaaaaa
     4861 tctgaaacac tgtaatttat ccttccagca taataagtat ctgtatatga ttttaattta
     4921 actactatta agtctgattc tgtagtaata cctctgtact gagaatttat ccttccattt
     4981 ccaactaata taccagaaac taaagttcca gtacctatat tatcttgact caaactccca
     5041 tcatttctat ttatggctat atttaactca cttcttgtaa attcacttcc aaaaataaaa
     5101 ccttctggag gtggatttgt atttgcttct tggtcccata aatataaaac tttactagtt
     5161 ccatcatcat ttataaaatc aggatgcaaa taatctatcc cagaatctat aactgctaat
     5221 aaaattcctc tccctgttat atcattatat ggattttcat aaatataatc agtttcagct
     5281 gctgttgtaa tggtttctcc attatttaca ttattagtta tttcaattag actgctcatt
     5341 ggttcagatt cttcccacca agcaacttgt attatattat ttaaaatagt ttcatcaaaa
     5401 tctaaaggta catatattac tgcaagctga ctatttaaaa ccatatacct atttattcca
     5461 ttttcttgta gggcagattc tatatcacct tgataaatta tacaataaga tttttccata
     5521 aatccctcct atcttaaaac atcaaacatt cctctaacat ccaagagccc atatccagaa
     5581 tttgtatttg gatatactgt gtttgaatct tttctagcac cagcttgcat aaaagttttt
     5641 attttttgca catatgcttg gtttggatat ctaccgtcaa caaaagtata ttgaaaatac
     5701 attgcagcag caccagctgc atgtgcactt gctgctgctg taccagttat agttgcatac
     5761 gtgttcccag gataagcagc tattatatta acacctggtg ctactatatc tggttttaac
     5821 ctgtcttcaa tagttggccc tcttgatgaa ctttgccata aactaccatt aatagtattg
     5881 taggctccta tggttattaa atcatcttga atcgcaggat aatttatagt gtaaaaaggg
     5941 tctacctctc taaatctagt tccacttttt aaaagttctc tatttggaag gtataagtta
     6001 tatcttcctg ttataatata aactcctact aatcgaatct tccatacacc tctttttgca
     6061 ttctttaaag taacattagt aaattgctgt ccagaaaaag ttgttgggta tatatatgtt
     6121 atagaatatt cagtcccctc taaatcaaat aaacctgtta ctttattata atttgaaatt
     6181 ccaacacttt tactttcttc accagtggga gatactatta ttacatcagc tttatctggt
     6241 ctatttaacc aaagttctaa tgataattct tcctcatctt catttaactc taattcaact
     6301 tcaacagaac ctccaacata tggtattatt ccagatgtat gtgtctgtgt attgccctca
     6361 tttcctgctc ctgccgttat acataaccct ctagtaaaga acgctttttc actattactc
     6421 ctattggtta aacctgctag gctactggta cctaaagaca tatttataac taatggtctt
     6481 ccaagttcaa aagctttttt atatgcatac tgagatgcag caaacagcat tgcactatta
     6541 taaaaatcat ctatcttgcc gagttttatt attattagct cagaatcttc tgctatacct
     6601 gcatattcac tatttacatt tccaagccct gcacatatcc cactcaacat agttccttga
     6661 ccaacttcat cctgagatag actagggtca ttttctgcga tagctcgatt tatatcttct
     6721 cgtgtatatt ctgtcccaat ataaaaacca tcaggaggag ttccttcttt tgtttggtcc
     6781 cataaatata ctatttttga agtgccatct ggataaataa aatctgggtg tagatagtct
     6841 attcctgtat ctgcaattga tattagagtc cctcttcctg ttatattaat attaggatta
     6901 tttttaaaga aatttacgcc catttcttct gttgcaacta cacctccaaa agtacctcga
     6961 ttaatttctc caagtaatgt catctttgta gtcgatacag ttcttatgat tgaaggaagt
     7021 tgcaaaactc gttgtatgct attatagtct gtattattta tagatagtac acctaatgaa
     7081 tcacttatct taaaaaacct ttgactcata cctaacccag ctagttcttc ttcaaattca
     7141 ggagtatgga ctatatctat tcctgatagt attcctgatt gtctacttgc atttttactt
     7201 atataaattt cattctctaa gtcattatgt atttgcatat tataaccatc tataaactta
     7261 taactatttt cctcatcttt aaatgactta ttaatacttc tatatccaat atcctctgtt
     7321 tctaaatctt gatttatatt agatagagtg tacaaatcta tatctgataa atttaaaaaa
     7381 ccaaatcctg atgaattatt tggataagat tgattactta atctccttgc tccctttaga
     7441 agtaatgccc ttaatttctg tgaatacaaa aataaatcat ttccatttac aattccccat
     7501 tccataaaca atgaacaaac ccctgtaaca tgaggtgtag ccatgctagt tccagttaat
     7561 gctccacttg tacctcctgg taaaaaagat actatatctt cacctggagc taataaatca
     7621 ggtttaaaaa cacctaattg tgtatctcct tcaccagaaa atatagatac tatatctgtt
     7681 cttgaattaa aactacctac tgttataact ctacttgctg ttccaggaac tgttacagta
     7741 agctcttgag taggaatcag aaatctagta tttctattta gaccttcaga agttggcaaa
     7801 tatatattta cattaccagt tactatatct attggttcaa atacaatttt ccataaccca
     7861 ggagttatct gtgtatttga acttaattgt agagtaactc ttcttgtgag agaatacggt
     7921 gctatagggt aaaaataacc agttattcta gtttcaccca aagtattcct tatctcacct
     7981 gaagttagag agattgcttg tgtttgatta tttgatgggt ttaccaaatg tacactaaaa
     8041 tcatcaacaa aatctggcca tatatttatg tttaatatac gctctccttc cccaactata
     8101 aactcaactt cttctgtaat attattttga agcctaatcc ttttatgacc tcctttatct
     8161 gcattatttc cagctgctac aactatattg tttttccaaa ataaacacat atcatctata
     8221 tattgttcaa ataaagatgt tcctctatgt gaaccttcat tacttccata acttatattt
     8281 aatgtaacag gcattcttaa ttctaatgcc ctatctaaaa taaatttaat tgccctcata
     8341 aactctgtac ttctcgaaaa aatatcggtt tgtatatttc caactctaac cactattatt
     8401 ctggcatcac ttgcaatagt tgcacaaata cctgcaacat gtgtcccatg taagctagtt
     8461 gttgaaatag gtatatacat acttccatct attgcattat ttatatcttc attagtatat
     8521 aatgttcctt ctctaaaacc ttctggtgga tttccttgaa tagattggtc ccaataatat
     8581 aatatcttag accttccatc actatctcta aacacaggta gagtataatc tattcccgaa
     8641 tcaattatac caattatagt tccttttcca gttaaaccag ttctattttt aaatcctgtt
     8701 attcctgtac ttgaaaaact ttgcacatcc tgagtctgta atataaaagg cttttctata
     8761 aattcaattt ctggataagt taaaagtata tttacatctt cttcattact tgatgttatt
     8821 attgcatatg atgaattaag tatctctact gatacaccta attcttcttc taacctcaat
     8881 atatctccat tgtattttac aattaattca taatttatta taatcacctc gacttctaaa
     8941 attattatta atgtatatgt tttgtgtgag tctaaaatgt attagactat ataatttttt
     9001 aagtttcagt tatacaattt ataataggta tattaaaaaa ataaataatg atggtacaat
     9061 tttgaataaa tcataccatc atttatattt actttatctt gaaaccatta aatttttcaa
     9121 tgctttctca agacctttta cagtcaatgg aaacatatta aattcacttt gtatcattct
     9181 ttgagtttga taccaataat taattgaatc ccatccatct ctaagttctg gattcatcca
     9241 tacaatttta ctgtactttc tcttaaacct tttaagccac tctataccag gtgtgtaatt
     9301 atatggacca cgatagttac caccaatatg aagcaactca gaaggcgaca tagaagcatc
     9361 tccaacaatt ataactttat agtttttatc tatatttttt aatatccatt cagtattaat
     9421 agatttactt aaccaacatt caggtgttgt aaataatcta tcataaatac agttatggaa
     9481 ataatatatc tttacatctt taaaatgatt tgatttactt acagactgaa ataatgtatt
     9541 acataaagtg ctataccctc tcattgaacc acctgaatcc ataagcaata aaagcttcac
     9601 tgtatttttt cttggcttct caaaaaataa ctttaaatat cctgcattat tacaagtctc
     9661 ttcaatagtc ttatccaaat caagctctgt ctttggagca tctattctag tagaaaattg
     9721 acgaagtctc tttagtgcaa cttgaaattg acgcatattt aaaacttcat catctctaaa
     9781 atctctatat tttctttctc ctgcaacttc aagaacagct ctaccataag tagaatttcc
     9841 acctatacgt ataccagttt gcccttttcc tgaatgtcct aactctgaag tgcctcctgt
     9901 gcctatccaa taattaccac cattatgagg agaatcttgt tcttttattc gttcctccaa
     9961 tttagctctt aatttatcaa gatttatatt tggattttgt tctaatcttt caaactcttc
    10021 taaatctata tctggttttt taagccaatt cataatctct tctggaattt cttcttggtg
    10081 ttctatacct ttaaaatact ccaaaaacac tgcatcaaac ttatcaaaat cactttcact
    10141 ttttattaaa atcatcctgc aaagatagta aaaatttgaa aagtttgaat agcacagtcc
    10201 tttatccaat gcttcaatta aagtcatcca ttcatttaaa gatacattta atcctcttga
    10261 cctcaataaa taaaaaaatt caataaacat aattatccag ctcctattaa ctataagtgt
    10321 ctttgcatag attcaatatc ctcattattc ttaagtagta ttcctgcaaa aggaacttct
    10381 ttttctattt tttcaatagg cattccactc aaagttaatg cttgtatcca gtctattaac
    10441 tctgatgtac taggcttttt ttgtatgtcc tttaaacttc taatccaata aaaagtagtc
    10501 attacttgtt ccaataggtg ttcttctact ttatcaaaat gtactttaac tatttcttcc
    10561 atcatgtctc tatcaggaaa ttctatataa tgaaatatgc atcttcttaa aaaagcatct
    10621 ggaagttctt tttccgcatt tgaagttata ataactattg gtctttgttt agctcttaca
    10681 gtttcttttg tctcatttat gtaaaactcc attttatcca attcccaaag caagtcattt
    10741 ggaaattcta aatccgcctt atctatttca tctattaaaa gaattacttg ttgatttgag
    10801 ctaaaggcct ctcctagttt accatatttt atgtacttgc taatatcatc tacaccttct
    10861 cctccaaatt ggctatcata aagtctttga acagtatcat atacataaag cccttcttgt
    10921 gcttttgtag ttgattttat attccaaatt actaaatctt tttttaattc atttgaaatt
    10981 gcttgtgcta gcatagtttt acctgttcca ggttcgccct taatcaaaag tggcttttca
    11041 agagcaatag caacattaac tgatgccatc aattctggtg atactacata atcactactt
    11101 cctctaaagt tagatatctc tttttttatc ataaatccta ctccttttaa acagtatttt
    11161 ctaaatgtat atagacttta ttactatttt tgatataaaa ataataatac aatacatttg
    11221 tatagtatta ttctaaaatt ctatatctaa ataaaatcac ctctaaaata tttaattttt
    11281 atctttattt gtcataatta aatgaaatat atattttaaa gaataataaa agatagctta
    11341 ttgtacaaaa tttaaattat aaaaacttag tttaatcaaa ggcacca
//
LOCUS       DBGJFU010000121        11296 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.122, whole genome shotgun sequence.
ACCESSION   DBGJFU010000121 DBGJFU010000000
VERSION     DBGJFU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.122"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(176..937)
                     /locus_tag="ACOIAS_003014"
     CDS             complement(176..937)
                     /locus_tag="ACOIAS_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2702708.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            1156..1632
                     /locus_tag="ACOIAS_003015"
     CDS             1156..1632
                     /locus_tag="ACOIAS_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGZ2702709.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1730..1882
                     /locus_tag="ACOIAS_003016"
     CDS             1730..1882
                     /locus_tag="ACOIAS_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HGZ2702710.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(2042..2230)
                     /gene="rpmB"
                     /locus_tag="ACOIAS_003017"
     CDS             complement(2042..2230)
                     /gene="rpmB"
                     /locus_tag="ACOIAS_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGZ2702711.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2591..2941
                     /locus_tag="ACOIAS_003018"
     CDS             2591..2941
                     /locus_tag="ACOIAS_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGZ2702712.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            2960..4603
                     /locus_tag="ACOIAS_003019"
     CDS             2960..4603
                     /locus_tag="ACOIAS_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HGZ2702713.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4684..6741
                     /gene="recG"
                     /locus_tag="ACOIAS_003020"
     CDS             4684..6741
                     /gene="recG"
                     /locus_tag="ACOIAS_003020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGZ2702714.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6800..7354
                     /gene="rsmD"
                     /locus_tag="ACOIAS_003021"
     CDS             6800..7354
                     /gene="rsmD"
                     /locus_tag="ACOIAS_003021"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HGZ2702715.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7356..7853
                     /gene="coaD"
                     /locus_tag="ACOIAS_003022"
     CDS             7356..7853
                     /gene="coaD"
                     /locus_tag="ACOIAS_003022"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGZ2702716.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7857..8381
                     /locus_tag="ACOIAS_003023"
     CDS             7857..8381
                     /locus_tag="ACOIAS_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702717.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            8725..10644
                     /locus_tag="ACOIAS_003024"
     CDS             8725..10644
                     /locus_tag="ACOIAS_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2702718.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSIIELINKQN"
     gene            10657..10944
                     /locus_tag="ACOIAS_003025"
     CDS             10657..10944
                     /locus_tag="ACOIAS_003025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2702719.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
BASE COUNT         4560 a         1228 c         1968 g         3540 t
ORIGIN      
        1 ttgttattat tttgtattga ttataattat tatacaataa agattctgtc tcaaaataga
       61 gagagaaacc tgtttttcat tgataaaatg ataaaaatat atctgatttc atacaaaaaa
      121 gagtgtctca tgaactaaaa agttcatttt gagacaccac ctttattatt ttatattatt
      181 tatctcttgt atatattact atagcatccc ttaaaaactt agctaatcca ggttgttctc
      241 tatcataata tgatgtaaaa cgttcatcat atacatacat ctgtgctaga gaggaatgag
      301 cttctttaga gtatgtgttc caagtgtaac tcaaccattt atgatgtaat tttgcaactt
      361 cctgagccag ttcactagag gcatcaccag ttttaaaagc ttttttaagt ttactaatta
      421 tttcgatatt taggttttgc caattttcat aatcttgctt agacatattt ttaagttttt
      481 tatttgatgt atctataata tctttaccat atttctctct aatttcagtt ccataatttt
      541 tttcattttc atcaatcatc ttttctttaa aaccttcaaa tttttctata tctgtcatag
      601 tgtattttcc ctccttcaga gctatagttt tagttacatt ttctattagt aaatcaattt
      661 gctttctttt ggctagtaat ttattatggt gttcttttaa cgcacttaaa ctatcgaagt
      721 ttggtgaatt tataatgttt tttatatttt ctaagttaat tcctaattct ctataaaaaa
      781 gtatttgttg aagtttatta acttcatttt gaccatatat acgataacct gaagaattta
      841 ttttaagtgg tttaagaaga cctatttcat cataatatct aagtgttcta gtacttatac
      901 cagctagttt acttaatttt tgaactgtat attccataag tagtacctcc tataaattaa
      961 atatacactt ttacgcaacg taaatgtcaa tacattaaaa tataaaaatt tatatctttt
     1021 aataaaaata taaaaaaata tctttactgg atattttaca cacaactgtc aatactcact
     1081 aatgaaaatt aaaaaataaa atttatagac tatctttact aaaaaataga gggtctatag
     1141 tctaggagga actgtatgaa tttaaaaaga agcaatgaaa aacgagaaga gtacagaaga
     1201 atgtattcag ataaagaaag ttttttaagt ttgattcaaa attttacaag tgtctcaata
     1261 gcaaaagaat taactcttaa aaatatagag ttagaaacat cttttatatg tgaatataaa
     1321 ggaaaagaag ttgatataat ttataaggta ttttctaaaa gtggcaaagt atcacattat
     1381 atagtgttag aatttcaaac agaaatggat actgaaattg tcccaagatt gaaatcgtat
     1441 agagaacaaa tttggaaaag ttttattatg aaaaaaagcc ttgaagaaat agaagataaa
     1501 aattttaaac ttcctaaagt tataccagtt gttttatata gtggacctga aaggttaagt
     1561 agaaaaaaag gaattttgga tattgtagaa tctatatcgg agttcagtaa taattctagt
     1621 atgaaaaatt aaaattacat aatgtgttta ttggtctaaa atggaaaact gtcgcataaa
     1681 cttgaatatg aaaatattta tagatacagg ttctatgtgg aggtgttcca tgagaaataa
     1741 caagtctaag gtattgctag gaatactttg tctagcaaca ggaatggctg taattctgtc
     1801 aatgttgctt cctggctgga tttggtcagc acttaccgca ttaatactta ttggatgtgg
     1861 agcattatta tttttttgtt aaaatagttt taactataaa gtgtggggtg agtattgatg
     1921 aaagtagtaa caataaaatt accaaaatgg atttcaagta taatcctaaa atttatgaga
     1981 aagagtaaaa atgattaaat taaaaagcct acttacggaa gtaggctttt aaacgtggtt
     2041 actaagctct ttcaatttta ccagaacgta aacatctagt acaaactttt actctcttag
     2101 gagctccatc tataatcgct cttacgcttc ttaagttagc tgaccatgtt cttttattat
     2161 gtttatttga gtgtgatact tggtttccag aaaccttacc tttaccacat acgctacata
     2221 cttttgccat ctacgcacac ctccttaaaa tgataactat ttaaagacta aaaacatatt
     2281 tatattatca taaataacaa aaatttgcaa tagaaatata tatttgcctt acacatattg
     2341 aagaatttct tattatcata taaaattata tatataattt tatataaagt aaatatattt
     2401 ggaaaataat tattattttt ctttgaatat atatactata gaagaaattt tatccaaaaa
     2461 cttttctagg tttaaattat accatattta tagtatgata tgattataat tttatataat
     2521 aaataagtaa aaatagtaaa aaataaatta agaaatgttt ttaataaaaa taagcttaag
     2581 ggggtcaacc atgagtgcaa aggtaacgaa tcaatatgga aatatagaaa tagataatca
     2641 agtaatagct caagtcgtat atagagctgc aatggaaagt tatggcttag taggtttagc
     2701 atataagtct aaaggaatag tagagctatt aaaaggcgag aacgcaacta aaggtgtaag
     2761 agttgaggaa gtagaagaag atgccatagc aatcgaactt tatgttataa tacaatatgg
     2821 tacaaatata tctactgttg ccaataatat aatagataga gtaaaatatg tagttgaaaa
     2881 aatgactggc gtaagagtta ctagaattga tattaatgta caaggaataa gagttaagta
     2941 atttcatagg aggaaacaaa tgattcaata tatagatggt aaaaggttaa gggagatgtt
     3001 tatttcaggt gcaaacaatc tccaaaacaa taaagagtta gttgacaagt taaatgtatt
     3061 ccctgtacca gatggagata caggaactaa tatgtcatta acaatatctt atgccttaaa
     3121 agaattagca aaagttgaaa atgataatat atcagatatc ggaaaagcat tatctaaagg
     3181 ttctttaatg ggagctagag gaaactcagg agttatactt tcacaaataa tcagaggtat
     3241 agctaaatct atagaaggaa aaagtaaatt atcaacagaa gatttagcaa aagcttttaa
     3301 aaatggttca gacacagcat ataaagctgt aataaaacca attgaaggaa ctatacttac
     3361 tgtagttaga gaaagtggag agtttgcaat aaaaactgcg aaaaaagaga aagatgtagt
     3421 aaagttttta agtatgcttg tgaaggaatc aaattcttca ttagaaagaa ctccagacct
     3481 tctaaagaat ctaaaagaag ctggtgtggt tgactctggt ggaaagggtc ttgtacttat
     3541 atatgaagga atgttagctt ctataaaagg cgataacata gaaataaaaa atgttgacct
     3601 agatactaat atttcaacaa gtatggattt tgcaaagagt actactagca cagatgatat
     3661 aaaatattgc tattgtacag aatttatttt agaaagttct aaggtagaag atactaaaat
     3721 aagagacata atgatggctt atggtgatag cctagcagta gttggagatg atggagtcat
     3781 aaaggtacat gtacacacta atgacccagg aaatgtactt caagaagctc taaagtacgg
     3841 tcaattatta actataaaaa tagaaaatat gaagttacaa catgaaaata cattactgga
     3901 tgtagaagaa aagaaagaaa atgatagtga acctttagaa gaagaaaaag agtttgggtt
     3961 tatagctact tctatgggtg aaggtttggc aaatatattt aaagatttcg gagttgacca
     4021 tataatagaa ggtggtcaaa ctatgaatcc aagtactgaa gattttatga atgctataaa
     4081 gcatataaat gctaaaaata tatttatttt cccaaataac agcaatataa taatggctgc
     4141 taatcaggca aaagaattga gtgataaaaa tataatagtt atacctacaa aaaatacgcc
     4201 tcaaggattt gctgcattag taacttttaa tggagaatta agcgaagatg aaaataaaga
     4261 agctatgatg aatgctttaa actcagttaa atcaggtcaa gtaacttttg ctgttaggga
     4321 tacagtgatg aacgaaattg atgttaaaga aggtaatata attggaattg cagaagggaa
     4381 tttattatca gcaggagatt atgtggatga agtaacatca aaccttattg aaaagttggt
     4441 tgatgaagat actgctataa taacattatt ctttggtgaa gatgttacag aatctcaagc
     4501 aaatgaacta cgtacttctt tagaagaaaa gtttgaagat gtagatgtag agttatatta
     4561 tggaggacaa cctctttatt attacttaat ttcagttgaa taatgattat aagaacctgt
     4621 atgaccaagt agcttttata aaattggttg tacaggattt ttgtgtagta ggtgatatat
     4681 ttgttggatt tatataaaga tgttcaatat gttaagggaa taggacctaa aaaagcagat
     4741 aaattaaata aactgggtat atttacacta aaagacctat tgtattattt tccaagacaa
     4801 tttgaagata gaaataatct aaagaaaata gcacaattag aagatggtga gaaagttacg
     4861 ataaaagcag taatatcaag tataaataca tttagcccaa aagaaggtat gactttaaca
     4921 aagattgatg taaaagatga gacaggctcg gctaaattag tgttttttaa taagagttat
     4981 ataaaaaata cttttcgtcc aggagattca atattagttt ttgggaaggt taagaaaaaa
     5041 tttaataact tagaacttac atcatgtgaa ttagaatatc ttactaattc acctaagaat
     5101 acatgtagat ttatgcctgt gtaccagctt acatacggtg ttacaaataa agaaattatg
     5161 agtataatta gaacagtact tgaagataaa gaattaatta tacaagaata tatgccacaa
     5221 agaattatag aaaaatatag gctatgtagt atagattttg cagttagaaa catacattca
     5281 ccaagtagta aggaatcatt aaaaatagct ttatatagaa ttgtatttga agaattactt
     5341 atactacaac tagggttgtt tgtatttaaa agtggcagaa ataaagaaga tggaataaag
     5401 tttgaaacaa gtaaagactt aaaaaaaata ataagtgctt taccatttaa actgactaaa
     5461 gctcaaaaca gggcattaga tgaaattata caagatatga atttagagaa aataatgaat
     5521 agattggtac aaggtgatgt agggagtggt aaaacagtag tagctctctt agcacttgca
     5581 aattgtgttt taaatggtta tcagggcgct ttgatggcac ctacagagat attagcaggg
     5641 caacattata tttcacttac tgagtctcta aaagattttg gaataaatgt aggtctatta
     5701 ataggtagtc ttacaaaaaa acaaaaggat acagtattgg aacaaataaa gaataatgag
     5761 attgatatat taataggtac acatgcacta attgaagata aggttgaatt taataatatt
     5821 ggtttagtta taacagatga acaacataga tttggtgtta tgcaaaggag caaactttca
     5881 ttgaaaggag caaatcctga tatattagta atgacagcaa caccaatacc aagaacttta
     5941 gcacttatac tctatggaga tttagatatt tctattatag atgaacttcc tccaggaaga
     6001 cagccaatag aaacaattgc tattgaaaaa agtaagcgag atagagcata taataacctt
     6061 gtaagaagag aggttgaatc aggaagacaa gtgtacattg tatgtccctt ggtagaagaa
     6121 agtgaagcca tagaagccaa atcagcagtt gaacttgttg aggagttaag agcggaatat
     6181 tttcacgact taaggcttgg gttgcttcat ggtaaaatga agtcaagtga aaaagatgaa
     6241 gtaatgagac tttttaaaga taaagaaata gatattttag tttcaacaac agtaatagaa
     6301 gttggtgtca atgtgccaaa tgcaacatta atgataatag aaaatgcaga aagatttgga
     6361 cttgcacaat tacatcagct tagagggcga gttggaagag gtagtcataa atcgtattgt
     6421 gtactaattt atgattctaa aacagatgta tgtagacaaa gaatggctat aatggaagag
     6481 accaatgatg gttttaagat ttcagagaag gatttagaaa taagaggacc tggggaattt
     6541 tttgggacaa ggcagcatgg tcttccagaa cttaaggtag caaacttatt taagcatata
     6601 aaaatactga agttagcaca gcaagaagct cgatatatat tgggtgaaga taataatcta
     6661 caattaaaag aaaacatggc actcaaaaaa gaaattatag ataaatttaa agatacattg
     6721 aaagaaattt cattaaatta attttaaatt gtgttaaaat attactaatg taataatgtg
     6781 ctgaagggag attgtcatat tgagagtaat ttcaggaaaa gcgagaggat taaaactaaa
     6841 tactccaaaa aatgaagatg taaggccaac tacagataga gtaaaagagt ctctatttaa
     6901 tatgataaat tcatatataa tggaaagtga agttctggat ttatttgctg ggactggttc
     6961 tttaggaata gagtgcttat ctaggggtgc caagtcatgt acttttgtag atatcagtaa
     7021 ggaaagcata gatattgtta agtccaatat aaaaaaggca agagttgaaa gtgaaagtat
     7081 tattttaaac cttgatttta agactgcaat agataaactt aaattacaaa atagtaaatt
     7141 tgacataatt tttatggacc ctccgtatta taaaaatatg tttatagaag ctattgaaaa
     7201 aattgataat tctaatctat tgaacgaaga tggaataata gttgtagaac atgatacgaa
     7261 tgatttattt ccagataaga tatgtaaatt ggagaaaact agagataaga aatatggaaa
     7321 tactacatta actttttata agatggaggc ataatatgga aaataaacct agaaaagcta
     7381 tatttgcagg aagttttgac ccaattacta atggacattt agatattatt tgcagggctt
     7441 cgaagttatt tgacgaatta caaataggag tattaaataa tcctaataaa aagggtctat
     7501 ttagctttga tgaaagagta aaacttatag aaaaaagtac aagccattta aataatataa
     7561 aagttgtgac ttttgatggt ttattaataa attactgtca ggaaaatggg attggagcat
     7621 tagttagagg tgtgagaagt ggtgctgatg ttgattatga acttcagatg gcgcatatga
     7681 atagagagct taatccagat atagagacta taattttacc aagttgtaca aaatattcat
     7741 ttataagttc atctttaata aaagaggttt tgctctttga tgctgatata aaaaatttgg
     7801 ttccaaagat tgttttggaa gaattaaaga aaaaaactag tgggggtaat tgatatatga
     7861 agatagattt agaaatgatt gaactatttg agcagttaga ggagatattt agaagtgctt
     7921 ctacaatccc tttctcacat aaatgcacag tggataaaga tgaggtttta gcaataatta
     7981 atgatataag aactttaata ccagaagaag taacacaggc agtatggatt aataaagaaa
     8041 gaaataaaat aataagtcaa gctaagcaag atgctacaaa tatagttgaa caagcgaaaa
     8101 aagaagctga gagaatacaa caagaatatc aagataatat agaggaatta aagaaaaatt
     8161 ctgaagatgt agtaaaggca tatgtagaat ccagtgaacc tgttgttcaa gcagaccaaa
     8221 gagcaaaaga tatagttgat agagctgaga gaatagccaa tgagataaga attgggtcaa
     8281 ttgaatatgc agaggatgtg ctttctagtg ttgagtataa tttaagagaa atattagaag
     8341 aagtaaaaag agataaagct gaacttagac ctaaaaaata aattttaaat aaataaaaat
     8401 ggagctatca cacaaaaaat tccaatttat gtgtgatagc tctcttttaa tatagcaatt
     8461 ttttgttata taattgtgct ttttagatta cttatatctt attttgatat tataatgtag
     8521 tttaatcttt tatttgttga aatcaactta tttatttgta taaataatat aggttatttt
     8581 caaatttttt taataatttt accactgtct tcttttagat tagagttaga ctgtgcaaaa
     8641 ttataaaata atactaaatc atgtgcaatt atttggtatg gcactacatg tagaaaatat
     8701 agataataaa attaaggagg tagtatgaag gaaagggcta aaaaaatatt agattttatt
     8761 attaaaaata acaatgtaac atcacaagaa ctacaagaaa agtttaatgt ttcaaagaga
     8821 actatttatt acgatattct agctataaat gaacaactag gaaaaagtgg taatataaaa
     8881 aatgtaaaac acaaatttat atttgaaggt aatttatgtg atgcaaggaa aataatttca
     8941 actgaagaag ataaattttt agatagtgat tacagaaaaa cttttatatt aaacaagata
     9001 ttacttggcg aaaaaatatc aatagaaaaa ctaacaaatg aaatgttact ttcaaaaaac
     9061 acagtagtac aaacaataac tgatgtaaaa aaataccttc aaacaatggg gttaagatta
     9121 gagtataagg gaaagtacaa aattataggt gatgaatacg ttattagaga gctgtttctg
     9181 ataattgttc aagaaaatgt actggaaatt aattctataa gtgaggaagt aagtagtttc
     9241 gatacaaaag gacatataaa acttactgat tattctttat taaatctaac taagtttgtg
     9301 gaatttctaa ataaaaggat aagagatggg aaaacattat actcatataa atatctaaat
     9361 gaagctaaaa aaattagtta ctttagtaat tgtaaagaac ttttatgtga agaagctaat
     9421 gaaaatgaac aagcctatat atgcacttac ataagttcac ttcccagctt aaatagtgaa
     9481 gttaaggaag atgttgttga agaatatgta gataaactta tagataagtt tgaagtaaat
     9541 acagcaataa aattagaaag taagcatgag tttaagaaaa atattttaag gcacctccac
     9601 tcttcttaca acagaatacg atttaagttt cctattagaa atccaatgtt agatgagact
     9661 aagtataaac atgagtcact ttataaaatt ataaaatcaa ttatagagaa tgaagaagag
     9721 tttcctgtat ttgaggggat aagagaagaa gaaataggat ttatagcagc gtattttgga
     9781 ggatatttaa gagggtctag ggataatggt ctaagaagaa ataaagtctt actagtttgt
     9841 cctaatggtc ttatggtatc aaagagtttg gaaatacaac tatataaata cataccaacc
     9901 atagaaatag taggtatagt atccattaag caactaaaag aagtaaatgt atactacgat
     9961 tacataatta caactataga tatacaaaat gtaaacaacg taattgttgt aaatcctctt
    10021 ttaacatcat cagatgttca attacttatg aacaaactaa taagtgtaaa agaaaacgag
    10081 aagtatttca atcttgaatt aataatacaa gctataagaa aaaatggagt tatcaataat
    10141 gaagaagcac taaaagctga tttattaaac ataatacaca agatagatga aggagagatg
    10201 tatcaaccaa tgttaaagga attaattaat gcagaaaggg tcaatataat aaaaaatgtt
    10261 agagattgga aagaagcaat aaaaatagcc tcaaagccat tgttagaaga taactctata
    10321 gaagagttat atatagaaaa tatgataaag tcagttgaga aatatggacc atatattgtt
    10381 ttagctgata gatttgctct accacatgca agttctaaag aaggagtaaa caagcttgct
    10441 atgtctttac taatagtaga agatgaagtt gatttattag gaaaaccagt aaatatattt
    10501 atggtattag ctgctgtaga taacactact cacattagag cattagcatc actatcagag
    10561 atgatgtacg aggaagaaaa tgtaaaacta ataattaatg gtgataaaag ttcaataata
    10621 gaacttataa ataagcaaaa ttagggggaa tgaaaaatga agatattagc agtatgtggt
    10681 tttggtgtag gttcatcaat ggtattaaaa atgacaatcc aaaaggctct aaagcagtta
    10741 ggtatagaat gtgaagtaga aaatacagat ataaattcag ctagaggtgc tgattgtgac
    10801 tgtatattca caagtcaaga attgttaaat gaattaaaag gaacttgtaa tgtagatgta
    10861 tatccagtta aaaagtacat ggatttagca gaagtcacag aagttgttca aactttttta
    10921 agtgataaaa atgataagaa atagtataag ttttaaaaag taaatttaag ttaatcagaa
    10981 taaaataaaa acttactaag ttcaaaaata gtaagaaaga gaggagggat ttagtggtag
    11041 aaaacttaaa cagttgacct atcactaaaa aatataatgc ttgattaaaa aatagctacg
    11101 cacactcgtg accttagtca tgagttagta gctaaaatag tcagcatata gggaaaccta
    11161 tatgtagaga taggatagaa cctatccaat actacttgaa ttgctggaaa cccctaaagc
    11221 taaccaaact aaaacataag gatgaaataa acctaagtgt gaaagttacg aaagtagaaa
    11281 aaattggtta gatggt
//
LOCUS       DBGJFU010000122        11038 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.123, whole genome shotgun sequence.
ACCESSION   DBGJFU010000122 DBGJFU010000000
VERSION     DBGJFU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.123"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..592)
                     /locus_tag="ACOIAS_003026"
     CDS             complement(<1..592)
                     /locus_tag="ACOIAS_003026"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGZ2702720.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSN"
     gene            complement(685..1017)
                     /locus_tag="ACOIAS_003027"
                     /pseudo
     CDS             complement(685..1017)
                     /locus_tag="ACOIAS_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            complement(1095..1298)
                     /locus_tag="ACOIAS_003028"
     CDS             complement(1095..1298)
                     /locus_tag="ACOIAS_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGZ2702721.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            1427..1636
                     /locus_tag="ACOIAS_003029"
     CDS             1427..1636
                     /locus_tag="ACOIAS_003029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702722.1"
                     /translation="MVQVTAKQPFQRLFTNHYIVALLIKVMAYSICWLSVSQTYLSTL
                     FSCQRTVGIDLSIKNHNSLWAFIDK"
     gene            complement(1688..1918)
                     /locus_tag="ACOIAS_003030"
     CDS             complement(1688..1918)
                     /locus_tag="ACOIAS_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702723.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1920..2327)
                     /locus_tag="ACOIAS_003031"
     CDS             complement(1920..2327)
                     /locus_tag="ACOIAS_003031"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2702724.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(2687..3136)
                     /locus_tag="ACOIAS_003032"
     CDS             complement(2687..3136)
                     /locus_tag="ACOIAS_003032"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2702725.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3150..4076)
                     /locus_tag="ACOIAS_003033"
     CDS             complement(3150..4076)
                     /locus_tag="ACOIAS_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702726.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4201..4395)
                     /locus_tag="ACOIAS_003034"
     CDS             complement(4201..4395)
                     /locus_tag="ACOIAS_003034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702727.1"
                     /translation="MDAPQYIEALEKTVANHEYKSCVNALQATINLYLELREELSQSP
                     IVRKVEAERVALQYFQDLIK"
     gene            complement(4755..5375)
                     /locus_tag="ACOIAS_003035"
     CDS             complement(4755..5375)
                     /locus_tag="ACOIAS_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2702728.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5594..6742)
                     /locus_tag="ACOIAS_003036"
                     /pseudo
     CDS             complement(5594..6742)
                     /locus_tag="ACOIAS_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6813..7649
                     /locus_tag="ACOIAS_003037"
                     /pseudo
     CDS             6813..7649
                     /locus_tag="ACOIAS_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8321..9088
                     /gene="aroD"
                     /locus_tag="ACOIAS_003038"
     CDS             8321..9088
                     /gene="aroD"
                     /locus_tag="ACOIAS_003038"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGZ2702729.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9416..10093)
                     /locus_tag="ACOIAS_003039"
     CDS             complement(9416..10093)
                     /locus_tag="ACOIAS_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702730.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10243..10560)
                     /locus_tag="ACOIAS_003040"
     CDS             complement(10243..10560)
                     /locus_tag="ACOIAS_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2702731.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10582..10920)
                     /locus_tag="ACOIAS_003041"
     CDS             complement(10582..10920)
                     /locus_tag="ACOIAS_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2702732.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3634 a         1767 c         1605 g         4032 t
ORIGIN      
        1 tgtttgattg aattttttta tatggccaat cccaccattt aatttctaat aattttgaaa
       61 ttgtatcatt attaaatctc ttttttataa tttttgcagg tactcctcca acaatagaat
      121 acggtggaac gtcattagta actactgctc ttgttccaat aattgcacca tcaccaatat
      181 ttacacctga cataattata gaatcataac ctatccacac atcatttcca attataatgt
      241 tacccttatt gtcccaagcg tccgttatat ggttgatatc gagtttccat tcttcataaa
      301 agatgggaaa agtatatgtg gattttgatt tcattgagtg atttccacta gtcattagaa
      361 attttgcatt acatgctatt gagcaaaact taccaattat taatttatca ttgtttatag
      421 ggtaatgata caatacattg tttttctcaa aatctcttgg gtcattataa aagtcattat
      481 atattgtgta atctccaact tctatatttt cccttgttat tacattttta agatatacag
      541 tttgataatc attacttctt ggatatacct tatttgaact tggaatagtc atatttaaat
      601 ctctccttta atatatatta tgtttttgac tattccacta ctacaataca tctcttcaca
      661 gaatcactcc ttgttaagaa tcttcataag ataatggcgt ccttttttag tgttaagtcg
      721 gatattcttt cttagtccat tctttttagc gtataactga caaactgtca ttttcttacc
      781 aatagtgtca atgctatcat caaggtcatt ttgtattgct ttttccttat cccttaatga
      841 tatatcatca cgttttccct ttgggggttt atcagtaggt acaagtttcc aagagtaaac
      901 aaattgtggt ttaccaaatg aatatacata cttatatgcg tatcttccat cttttctctg
      961 gctctctcca gttcttaaaa ttctatcttt attatctcgt cttttttcag ttgacatgat
     1021 aagaagctcc tctcaataac cgaattgagc ttgatacgtc atatgaatat tataacatat
     1081 aaggctcaat ttcattagat aaagtcaagt gtatcaacga ttttctcaaa ttgttttctc
     1141 tttatttgaa tgcgattgct attcataatg acccagccag aatcaaggtt ttcatcagca
     1201 agtttacgta attttttctc tccaatacga aagtattttg acgcttcttc aatcgtcaag
     1261 gtatattttt cccaaatagg tacatcagta ttgttcatag aatgtatcac tcctttctat
     1321 gttcgtagtt cagaagtaga tagatggctt tggtaagctc cattggtatt ttaaccaatc
     1381 ccattcttat gggcagaacc gtgtcataca ggtgtatcat tatactgtgg tacaggttac
     1441 ggcaaaacaa ccgttccaaa ggttattcac aaatcactat atagtagctc tcttgattaa
     1501 ggtcatggcg tatagtattt gttggctatc tgtatcacaa acgtatctat ctactttatt
     1561 cagttgtcaa agaacagtcg gcattgattt gtctataaaa aatcacaata gcttatgggc
     1621 ttttatagac aaataattag tgattctcca tgtaccaaat agcttgatac atggagagag
     1681 ggcgagatta agacaaatta aatctcaaaa tagcagtgat tagttttgtt cctagcattc
     1741 gctttgtttc ttcttctaca tgaatatagg gatttccgta ttcatcaaat aaagttttct
     1801 gtgaaagacg aatgatatag tgttcaaaat gtttaagtat tccgtttatt gcatttacat
     1861 cgccatgtac agcaagtaaa ataattgtaa aaggtaatac ttctttagta ggtttcattt
     1921 caatgttcct ccatgagttc tttcaactct tttagtgcat ttgttttgtg ataatgtatg
     1981 gtattttctg ctaaatccat caatgttgct atgtcagcat ttgtcatgtt gagaaagaat
     2041 gaaagtagaa ttatatcttg ttttcttttt gtaagttttc cgattgcttg tgccattaat
     2101 acatcttcaa ttcgtatgtt gtagtcgtaa gaaaccatac agatgtagtt agaatcataa
     2161 tcatctaaaa tactcaattt acttaattct gcaagtgaca aagattcgag agataccaaa
     2221 taatcatttc tgcgtttatt ttcgtcataa atatctctag catagttacg caaaactgtt
     2281 ttacagaaac tgtcgaattg atgttcaatt gtcttttcat caaccataag atttcaccct
     2341 ctttccatcg caaggagtta aataaccttg cttactccta tcacggtggg agggcgttaa
     2401 atcttcgata aatttcaaaa aaaagagaaa aaaatacaca ctctgcctat agggcaaaaa
     2461 tgtgtattct ttaaaacgta tttaatttat attggttgta agccaccacc atattgatgt
     2521 atggaaatat gcattggcta gcttttcata aaaatacaaa gatgcataag aaattaatag
     2581 aggggaaaaa cttattctgt tattaaaaag taggttaatt ctaataaatg ctattgcggt
     2641 cgaattaaac tatatacatg gcaatcaatc atgtttctat tctttattat gaattgtgac
     2701 agtattcttc aatcttaaaa ccacattttt ccaatacttt acttgaccct ctattgtagg
     2761 aatatacatt agcatgaatt ttgttaaggt tgtatttttc aaaggcaatt ttgcatattt
     2821 cttttactgc atttgttgct atccctttgc cccaatagtt cctagcaagc cagtaaccta
     2881 attcagcatt tttattaata gaggtatctt cttcaaatgt taggctgata cttccaatag
     2941 ccctttcttg ataaaaaata gcatagctaa gttgtttaac atctggagtt gccaaacaaa
     3001 aactaacaaa atctctagcg ttttgctccc tataagggtg ggggaagttg tcacgaagat
     3061 tctttgcaat ttctttatca ttcgcaaact caactaaatc ggtaatataa gcataattcc
     3121 actctataag ttccattttt cttctccttt tatgaaacag ctacaagaaa tctttctaag
     3181 ccaataccac ttatcaaaca gcgttctttt ttatttccaa gcatttgata agtccagtca
     3241 acaaatccac cgtcaactat ttcaacggtt tcatttcctt gtttgaccga taatttgaaa
     3301 ttgatacctt tataataatt attatcaaca tcatcattat gaattgataa ctctatatta
     3361 ggaaaaataa gtttgatagt ttcaaccatt ctgtcgaaaa atccgtcatt atctttgtat
     3421 ccgtttcttt tgcgtagaat aatagagaaa cgaacatttt ctatttcact taatatatct
     3481 ttataatagt ttaagtgctt ttctaacaac attttttcac aagtgtaaga ccctgtatct
     3541 ataccagatg aaaccataca atataagcca aaatgtgaat ataaaccttt acctacaaaa
     3601 ttttgtgctc ttattaccct agcagtagta gccatgtgga taggtatggt attattttct
     3661 tcttttcttt ttaatttatc agcaataatt attgccaaca tatttgaggg gtctgcaagt
     3721 gtttctgtcc ctcgtagagc acttactaca ttatattgat ctacggagcc aaaaacgcta
     3781 cagctaccta atggagcaga gggagaaagc ataatcgtct tgatatccat attgaatgct
     3841 tttctcaata attttgactc caaagcatga atttcttctg gatctatacc gctagggata
     3901 gtaaatctat ttagttggta tgactttaga atatcagttg cagttaaagt atttgcttgt
     3961 ctatcaaaca cttctaatag aagtgagttc aaatctgatt ttgatagagc tgataactta
     4021 tcaactatat tcttatcccc aattttattt gtaattctac ttaaaatatc actcatagtt
     4081 atcctccata ctgttacttt caaaataatt atagaatggt tacaacaact aatctaggtc
     4141 atttgaccaa aaatgaaaaa acactgaata tttactattc aatatttttt tactttgggt
     4201 ttatttgatt aaatcttgaa aatactgtaa tgcaaccctt tcagcttcaa cctttcggac
     4261 aataggactt tgtgataatt cctctcttaa ctcaagatac aggttgatag tagcctgcaa
     4321 tgcattaaca cagcttttat attcatggtt agcaaccgtc ttttctaatg cttcaatata
     4381 ttggggtgcg tccaattcaa tttttctaac tccacgaggt aatttaccta agagaagctg
     4441 tattaatggg gcaagtactg attgtcttaa aaaagataaa aaatcaattg tttcaaatag
     4501 ttctctacgt ccaatcttag tgcaagcgta atgaacccaa atccaaaatc tatcttcaat
     4561 ccactgtaag tctggagttg ggaagtatgc ttctttttta gtataacacc tctcactttc
     4621 ttttattagc tatcaatcca acataattat gcataggagt attatataat gaaaaaataa
     4681 aaaaaaatag gtcacttgac ccgaaataaa aaagcattga ataattatta ttcaatactt
     4741 ttttgaaatt atacttatgt tgtaggaaga ttctttaata cctctcgctg attaattaca
     4801 ctaaatcttt tgttttcata agcaattaga tgttcttgta cacattcatt tatagcacga
     4861 ttaatgcttc taattggagc gtttacttcg ctagcaagct gttttttggt aagcagattt
     4921 aattcacttc tataatagtg taaggatata cacctcagaa gtcgttcttt aacacttaaa
     4981 gatgataatt cttcaattaa ttcagcattt acaactaatt tactagatat ttctcgaatc
     5041 agaaattttg tagcttcaaa atctcccctt aaccagttta aaaaatcatc tctatgaagt
     5101 ttatatacat tacaatctgt gaaagcgaca atttcaacgg gttttttccc atcattaaat
     5161 tgttccattt ctccaaaaaa actaccactt ctataaagat gaatagttga gatgtttcct
     5221 ttcacattta agatgaatgc ttctgcaata ccgtcaatca taagaattac atattcattt
     5281 tcttgctcag caaaaagaat agtctctttc tttttatacg tttttttaac aagcttcttt
     5341 ttaatatctt cattcataaa atcaattaaa tgcatattca tccctcctta ccaaatcttt
     5401 gattaaatta tatcatcttt caattagtta ggtaagatag taaaaatata tttacataga
     5461 aactattcca ttattatttt ccagttgtca tttttctgca aagtaagctc aaactgagaa
     5521 agctgttgac aatagaacta gtaggaatat atgagcgaac catttcagta ttcaactgtt
     5581 gtaggttttc agtcagataa ttcttcaaac gttcaattcg tttgtcctgt gaatcttgcg
     5641 atgctctaac cagtttcata gatagcgttt taccttgtgc cacctgcaaa aaagaccatg
     5701 cttcgtcaag gtcaactatc ttgaagctag agcggtcagt atgaataaag tctaatgcaa
     5761 aggtactaat aacaatcaac atagcaacac taagaagttc tattgttgta tattcttcaa
     5821 agctagtgtt ggcatctggc aatacgaggt cagcaacttg aataatattc agctgtttct
     5881 ccaagctgat agactgcttc acatttccgt caccaaacag tagatgtgca aaatcatagt
     5941 ctacaaaact ttcgatatgg tcggctatgc tgttactgat agaagtatct tcttttcgta
     6001 attcatcaat gacaagtaat aaccctcgct gttcactttg ggttacattg cggattgctt
     6061 ttctaagcac tgggaactta tcactgtcac gactggagat accagcaagg aatgttagga
     6121 tatcaatcgc taaactttca gaatcatttt tacggctcaa aatgacgtaa gggtcaagca
     6181 atcctttgtt ttcatcatta ctggttaaat tgacaatgtt tatctcatgg gctatttctg
     6241 gtagcgtttc tttccatttg ccgcgttctg ctttagggtc aactatgacc gcctgtccgc
     6301 cgaatagtac cgcatagtaa acaagcatat tgttactgaa taatttacca ccacccaatg
     6361 aaccgataaa cgcagacgct aacacattgg taatagaacc ctttacccat tgactagcaa
     6421 gggcaggttt tagatagaca ttctttccag tgtccagatt gtagccaaga tagatatcct
     6481 catgttcgcc aagggtttgt gtcgctccaa aaccaagtcc agccaaaaaa tccgaagtga
     6541 catattgaat ataatcattg atgtaacgtt tgctgacagg aataaattcg ccatgcaatc
     6601 ccaacatatc cccaaaggga cgcacgagct tgagtgataa gtcgtcgtaa aagtctttga
     6661 cttcatcaca acgccttttt agctcgtcaa gatcctttgt agatacacgg acaacatagc
     6721 taagtttata catagattct ttacagttaa aagtctgttt ttaaaaaaat gtataaatta
     6781 taaatcaaaa tatagatact atcaatgacc cactaggtgc aaaaagcatt gatatggtta
     6841 aattatcaga cgctaaaagc tgggctatcc gaatgagtga agatgggtgt gcttataaat
     6901 ccattgacaa ttataagcgc tcactgaaag cgtcgttttt catggcaatt caagacgact
     6961 gtattagaaa gaatccattt aattttcagt taagtgatgt tttggaagat acaacagaac
     7021 caaaaatcgc tctaacacca gaacaggaaa tgggcttatt atcctttatg gagagcgata
     7081 agacttattg taaatattat gatgatgttg ttatcctctt agaaacagga ctacgcattt
     7141 ctgaattatg cggtttgact ttagaattgg atatgaaaaa taaagccatt atggtagacc
     7201 atcaattgtt gagtgatact aagattggct attatgtaga agttccaaag accacacagg
     7261 gtaagcgaga aattccaatg actgaaagag cttatcaatc atttgaacgt atcgtggaac
     7321 ggcgtaagaa cgctaaagca gaaccaatca ttgttgatgg ttaccagaac ttcctatttc
     7381 ttaaacaaga tggattacca aaacaagcaa ctcattatca aagcatgtta agagcgttag
     7441 tcaagaagta taataagacc catgaggaca agctaccaaa gattacgcca cataccttta
     7501 ggcatacact ctgtacaaat atggctaaca aaggaatgag tcctaatata ttgcaatata
     7561 ttatggggca taaaaacatt actatgacgc ttggctatta tgcacatggt tcatttagtt
     7621 cttcgagagc tgaaatggaa cggttataat cctagtagta tgactagagt ttactactta
     7681 tttactactt ttgatggcat tcttatacgg ttaaatgcca acttatgcaa agtatcttcc
     7741 aaaaagaaaa tgccgatact ccttgtagat acaggatata tcgacattta ccaacttatg
     7801 aaaagataat caaatttaat ataagttttt caaataaaaa gataccaaat tctttttcga
     7861 gaatctatat aacattactt aagcttggtt ggggtataaa caattgagta gctacttttg
     7921 tatggtgttc aactttagtc aaaactctta agtaatgcaa atgatttaga ttcattaata
     7981 ttcaatctct tcataagtag tgaaattcga gttatttctg cataattata ctatgattta
     8041 tagattaatt ctattagcat ccagaataat attgtttata tgagtaaaat ttaaagaata
     8101 ttgtgaaaat tataggatgt taaaataaat ttaggtaaaa aagaagtgtt tgtatattat
     8161 agagtacctt ttctaaaata tgtgggattg ttttaagaat tagaataaaa attattttta
     8221 aattagtatt tttataatgt atagtatttt taatgtataa ttaaattgtg cagaaaatag
     8281 aatttaaagt cctatagaat ttctgattat ggaggagaat atgaaaagaa aagttcaagt
     8341 aaaaaatatt actattggag aaggtagacc taaaatttgt gtaccaatta ttgggaaaaa
     8401 taaaaaagat attataaaag aggctaaaga gttaaaagat gcatgtttgg atattataga
     8461 gtggcgagtt gatttttttg agaatgtaga aaatattaaa gaagttaagg aagttctcta
     8521 tgaattaaga agttatatac atgatatccc tttattattc acatttagaa gtgtagtaga
     8581 agggggagaa aaattaatat caagagatta ttatactact ttgaataaag aaatttctaa
     8641 tacaggttta gtagatttaa tagatgtaga gttatttatg ggagatgagg tcatagatga
     8701 agtagttaat tttgctcata aaaaagaagt gaaagtaatt atatctaatc atgattttaa
     8761 taaaactcca aaaaaagaag agattgtatc tcgtttatgt agaatgcagg aattgggcgc
     8821 agatttaccc aaaatagctg ttatgccgca aaatgaaaaa gatgtgttag tgctacttga
     8881 agctacaaat gaaatgttta aaatttatgc agatagacca ataataacaa tgtcaatgtc
     8941 aggaatgggt gttataagta gattgtgtgg tgaaatattt ggttctgcat tgacttttgg
     9001 agctgcaaaa agtgtttcag caccagggca aatatcattt aaagagttaa attcagtact
     9061 taatctatta cataagagta ttaattaaat taattatata gaaatattag acggtatttt
     9121 tgaagatata aattaaatat aaaaaacttt tcagatagta aaatttaaat tgggcaatta
     9181 tatctgaaaa gcaactattt agtgtgtatt ttgaaagtga tatagaaaat cttataaatc
     9241 aactatggca tatttaaaat atgccattaa aacctactag acattagatt atagatggta
     9301 tactagcatt tctattattt atagatagta agttttcata ataactgttt tatctaaagt
     9361 agaagatata tttgtattat gtatatttgt cattattttt ttgtaattat gtatattaat
     9421 ggttatatat gtaatataat attatcactc tattttttgt atattgtaca ttacaacatt
     9481 tatatctatc atcatcaatt acagtagaat gtatgggata cttattttca cttaaccaat
     9541 atttttgcat attctttgtc caagactttg gaaacttcaa tactttcttg tttattataa
     9601 catcctcatc ttcaaaaata ttaacttctc tttctatttt tatttttctg ttgtatatct
     9661 tattgataga ttctttgtcg agttttatgt ctctatacat atcagggcaa tagcaacaaa
     9721 cataatttct tttttttcga cgtttaaatt tcacaacttt ctctatgcta atttctaatt
     9781 tttttgtacc atcttttata aatttaatat cttctatgtt ctcgcttgaa aaaatttctg
     9841 tcaatcttaa agtatctaca gtttctctaa ttatttgctt tttataagaa gagaatccat
     9901 atccaaaaga aaggtttaat ttattaggga tgtagccatt tttatattta aacatcttaa
     9961 ttttttccaa ttcagcatta tctttaaatt tcaacttttc atacctaaaa gttactttgc
    10021 taaaaagagt atattctaca tcaatatatt cttttgcaat cttaaattta tcgctacctt
    10081 caatcacaat catagcctat ctccaatcgt tttatattaa aataaggcca tagaacatgg
    10141 tctaatagta ctcattagca ctataagaca atgttaacac aaaaagacaa aaaaaggtta
    10201 tttatttcaa ataacctttt tttttatata aaatataaag aattatattt tattcttttt
    10261 tatgatgtct ataatctgtg atatcagatt tagttctctt gaatttaagt cttttaactc
    10321 ttcatttata tcattttcta aatatgtttt tccagtggaa gtaatatctt ttataagaac
    10381 atctgcagaa atatttaata tatttacaaa ttttatgaat gttttaagac ttggattctt
    10441 tttaccactt tcgatttgag ttatatacca aggcgatact ccaaccatat ttgcaaattc
    10501 ttttgaagtt aaatttgctt ttttccgttc ttctttaagt cttttaccta tataattcat
    10561 tattgttact atttccctca cttatatttc tttgtattta atgatgcact ttattaactc
    10621 tagcattgca atttgttctt tttcagttaa tttattaatc atttcataac attctaaagg
    10681 tacagagtta tcaacgcctt ctgttgtaat aatatttgca aataacaatt caagtggtat
    10741 gtctaaagca gtagcaattt tcattataat attgatagat gtatttgtct cagctctttc
    10801 aatctgacca atatatgttg gatgtaaacc tgccttttct gctagaaact cttgactata
    10861 tcccaatttt gttctataga cttttattct atctcctatt atttttccta tatttgccaa
    10921 ttattatccc tccactttag attatattca tagtttaagt tgttttatat agactattta
    10981 tactttttta gtctaaatat agactatata tgtttttata ctgaaataag tgctattt
//
LOCUS       DBGJFU010000123        10989 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.124, whole genome shotgun sequence.
ACCESSION   DBGJFU010000123 DBGJFU010000000
VERSION     DBGJFU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.124"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            65..649
                     /locus_tag="ACOIAS_003042"
     CDS             65..649
                     /locus_tag="ACOIAS_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HGZ2702733.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(753..1532)
                     /locus_tag="ACOIAS_003043"
     CDS             complement(753..1532)
                     /locus_tag="ACOIAS_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGZ2702734.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1590..2846)
                     /locus_tag="ACOIAS_003044"
     CDS             complement(1590..2846)
                     /locus_tag="ACOIAS_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HGZ2702735.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            3104..4003
                     /locus_tag="ACOIAS_003045"
     CDS             3104..4003
                     /locus_tag="ACOIAS_003045"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGZ2702736.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            4027..5475
                     /gene="ddlR"
                     /locus_tag="ACOIAS_003046"
     CDS             4027..5475
                     /gene="ddlR"
                     /locus_tag="ACOIAS_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HGZ2702737.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5558..6340
                     /locus_tag="ACOIAS_003047"
     CDS             5558..6340
                     /locus_tag="ACOIAS_003047"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGZ2702738.1"
                     /translation="MSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSNPNKKGNIK
                     SQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPI
                     SFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYD
                     AVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKVSTVY
                     ADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6366..8285
                     /locus_tag="ACOIAS_003048"
     CDS             6366..8285
                     /locus_tag="ACOIAS_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2702739.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8527..8874)
                     /locus_tag="ACOIAS_003049"
     CDS             complement(8527..8874)
                     /locus_tag="ACOIAS_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HGZ2702740.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(8861..10789)
                     /locus_tag="ACOIAS_003050"
     CDS             complement(8861..10789)
                     /locus_tag="ACOIAS_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HGZ2702741.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT         4100 a         1636 c         1502 g         3751 t
ORIGIN      
        1 tatcataaat atcatatcat aataatatat ttaataagtc taataaacta tacgaggtgt
       61 aaatatgtta atgaataccc attttattat ggcaaaatca atacttgata atatagatga
      121 aaataaaaca ttctttataa gcgaaaaaaa ttttatatat ggcaatataa agcctgatgc
      181 tttttctaaa tataaattaa aaaaacatta tttagatgag tcatttaata tgattgtttc
      241 taaaataaac tatctatgca gtcttaatat agatagtctt tctaaatact tttcagtaag
      301 tagacttagt caggaactag gagtcatatg tcatttttta tgtgattttt tctgtgtagc
      361 tcacagtgaa cgttgggagt ttaagcatag cttaaataaa catgttactt atgaaagaga
      421 acttggagca tttgcaaaag atgttgattt atctaaaatt aaatgtggta ctataaatag
      481 tagttttgag aagtttttta ctaaacttta tagcgattac aaaaagaaaa ctgattatat
      541 gaatgattta aaattttcag cttatacttg taatagtgtc ataaattata tacttgactc
      601 tattttatct aatacaataa aatcatatca tatagcaaat tgcggttaac ttaagaattt
      661 ttaaacttat ataaacttat aattgaatat aattaaaatg actgctctat agaatataat
      721 tttatacttc tctaggacag tcatttttta tactaactat ttatctccat tataacatct
      781 atggcacttt ttacactatc tactacataa tttgcacttt gctttacatc ttcccttgcc
      841 ttagacattg caaaactata tttaaatcct tctaacatgc aaatatcatt tggagaatcc
      901 ccaatagtag caacttcatc tggacttaca tttaacgttt ttattacatg ctctatactt
      961 cctctttttg acacactttt aggaactata tttaaagtat atgttccaga aaaatatatt
     1021 tctaagactt cactaaactc actttttaga taaagttcta ttctggataa ctcttccaaa
     1081 ttttcagaac atacgcctat atttcctata agattatcaa aactacttct tttttcaaag
     1141 tctgggtcaa ctataaagtc actagaataa tgtctaaaag cagtggtatc actacccaca
     1201 attacatcac ccttatattt aaaataaagt tgtgcatcac ttgactcaaa tctatcaatg
     1261 acttttttta ctaaattatc atctatcgtt cttttaaaaa ttacattata atctttatca
     1321 tatataaatg aaccattttc acatacatag tatccattca tatctaaact atctttgata
     1381 atattatgtg cttgtttaaa aattctacct gtagcaacat taaactgtac tccctttttt
     1441 tctatttctt caattgctac tatatcttct tttgttatgg ttccattttc atatagagtt
     1501 ccatctaaat cacaaaatat atgttttatc ataaattaaa agcctccaaa aacaatttta
     1561 tattttacta atactttaat caaaataact taaagctttt ctaattgtat tctcaactca
     1621 ttaagcaaat ctctagtagc tcttaatctt atctttcttc tatccccatt aaatatattt
     1681 ttctttacta ttgtttttcc atttatatat attgcagtat aaaccagtcc cacaggcttc
     1741 tctttggttc ctccaccagg tccagctact ccagttgtag atatacctat attagcttta
     1801 agtcctttag caacgccctc agccatttct atagctgttt gctcacttac tgctccaaac
     1861 tcttccaaag tttctctttt tactcctaat cttttcattt tagaatcatt agaatatgtt
     1921 acacaacctt caagaaatac agaggatata cctggatagt ttataagaga tgaagaaacc
     1981 aaacctccag tacaagattc agccactgct attgttagat ttttttctac aagtagctta
     2041 gaaacagcat cttctaaagc agtctcccct tcagatatgg aaatatctcc ttcagtatat
     2101 acatatttac caactctact ttttattttt tcttctactg gttttattaa tttaaaagct
     2161 tctctttcat tttcagcctt tgcagttatt cttattgtaa cttctaattc ttttgcatat
     2221 aaagcaactg ttggattagt ctgctcatta attatatcga gaatttctaa ttctagattt
     2281 gactcaccta ttccatataa tcttaaagtc ttagatacta gcatttcatt tgtgaattgt
     2341 tgtaagtaag gtttgacact ctcattaaac atagctttca tttctcttgg aggtccaggc
     2401 aatactatta taaatttacc atcttttttt aaaattgccc ctggagctgt accattatta
     2461 ttttttagta ctatagcatc ttttggaaag tacacttgtt tcatattatt ttcggccatt
     2521 tttttccctg ttttaacaaa gaaagattcc aaaacctcta gtgaaggttt atgtagttct
     2581 aatttctgac caaagtattc tgcagcagtt tcttttgtca tatcatcacc agttggccca
     2641 agaccacctg tagtaattac aaaatcactt ctctttaagc tttcatcaaa acattctaaa
     2701 agtctttgtt tgttatctcc aacagtactc tgatggtata cttctattcc caatgatgca
     2761 agttctttag ctaaaaattg agaatttgta tttactatat ctcccaataa tatttctgtt
     2821 cctacactta ttatttcagc tttcaaaatt tatcactcct tgatttatag ccattaagac
     2881 tagtcttaag ttcataacaa taattttagc aaaaataata acataaataa agttaaaaaa
     2941 tcatttaaac atatctttaa actagattat tattctaaaa ccacacataa tataaacttc
     3001 gtatatagtg gtactttcat cctcaactgt taattcatat aattaagtat gtaaataaaa
     3061 agtttacttt aaaataataa aaattaaaat aggagtgtat aatatgaaaa tagcagttat
     3121 aatgggtgga atatcatcag agagagaagt ttctttaaat tcaggaaaag agatatataa
     3181 taaccttgat aaaaataaat atgaagttgt aaaagtaata atagatgata aaaaagatat
     3241 atttacaaag ataccagaag atatagattt tgcaatactt gctttacatg gaaaatttgg
     3301 tgaagatgga tgtatacaat caatacttga gactatggat ataccatact ctggatgtgg
     3361 acctctttgc agtgggatgt gtatggataa aaatataact aaaaaaatgc ttagagattc
     3421 aaacctacct actgctccat gggttttagt aaaatcagtt gatgaaatag actatgatga
     3481 aatagataat ataggttatc cagtatttat aaaaccaaac tcaggaggtt caagtgtagc
     3541 cacattcttt attcattcta aggatgaagt tgaagaagca gttagaaaag gtctggaagt
     3601 tgatgaattt gtaatgattg aaaaatatat accaggtgga gaatatactt cattcatatt
     3661 aaatggagaa gtgttcccta caatatctat aaaatctgat tcaggattct ttgattatga
     3721 agctaagtac tcagttgaaa aaggtgcaaa ggaagaagtt gtttacttag atgaagaact
     3781 tcaaaaaaga gtaaacgaaa tttctgagac ttgttggaaa atttttaatt gcaaagcata
     3841 tgtaagagtt gatatgatta taagtgaagg tattccatat gttcttgaac taaatactct
     3901 tcctggaatg actcaaacta gtttaattcc tagaagtgct gctgctagag gtattaagta
     3961 cagtgaacta ttagataaac ttatagaata ctctttaaat tagatgaaat aatttgaggt
     4021 ggtaatttga tattttcaaa tctaaaacta aatgataatg aaccaattta tatacaactt
     4081 aaaaattata taagtgatat gatatcaaaa ggactaattc cagacaatag taagctccct
     4141 tctacaaggg agcttagtca gcttttacaa gttagccgaa attctgtagt tttaacttat
     4201 gaagaattaa aatcagaagg attaatctac tctatttcag gaaaaggtac atttgttaaa
     4261 tcaaagaata agtcttcaaa tactacatgg tcattaaact gggattgttt agagaataca
     4321 tactctaaaa aagccaatga attggacatt ataaaaagtg aaattccttg gagctctgac
     4381 ctgatttcct ttaaaagtat ttctccagat ggagatttat tcgacataga agaattaaaa
     4441 aaatcctttt taaatagaat ttcactagaa ggacataaat tactcaacta tggatatgca
     4501 caaggttata agccccttat tgactattta ctagaatata tgacaaataa gggtgctgat
     4561 ataagtaata aagatatact aataacaaat ggattcacag aaggctttga tattattatg
     4621 tcatctttta cccaagaaaa agactacata atatgtgaaa atcctactca taatactgct
     4681 ataaaaatta tgaaatctca taacctcaat atcttaggtg tagatataaa tgaagatggt
     4741 ttggacttca atatgctaga agacaattta gttaagtata aaaataaaat aaagtttgca
     4801 tacattacac cctcttatca caatcctaca ggaattgtaa tgactcctga aaataggtac
     4861 aaattctata atcttatgaa aaaatacaat atagcaatta tagaagatgg atttaatgaa
     4921 gaattactct acaatagttc tcatatattt cctatctgtt ccctagataa tatgaataat
     4981 ggggtcgtat atattggtag tttttcaaaa attttatttc ctggaatgag aattggatgg
     5041 atatttgcag ataaaaaagt aatcaataag ctagaaagtg taaaaaggtg cagaaacata
     5101 catgtatcct ttttggacca aggaatttta tttgattatt taagtaatgg aggttttgaa
     5161 aaatacatta aaaaaatacg taaattctat ggggacaaat ttaactttgc atataagtgt
     5221 ataaaaaaat atataaaaac agaatatatc ttgggtgatg gtggtttgca tatatttata
     5281 aaactaaaaa atatagatac tagagtttta ttagaaaaat gttatgaaaa aaatgttata
     5341 tttatgcctg gagatttatt ttttacagat aactctggtt ttgatacttt aagactcgga
     5401 ttttctaggt tgtcttttga agacattgaa attggaataa aaataattgg agaaactata
     5461 aatgaaatgt tctaggttaa ctaaatattg ttagcctagt ttatttttat gaggtataat
     5521 atatatagtt tatatgaatc taatactggg ggataatatg agtaaaaagt caaaacgaag
     5581 aaaaattaat aaattaaaat taacgattgt aataactgta ttatttcttg ctttattatc
     5641 tatatatgag atactgttct ctaatcccaa caaaaaaggt aatataaaga gtcaagaaac
     5701 tatgcaaaat aaaaataata caacagatga ttctaaaaat aatcaaagct ctaccaaaaa
     5761 gcaggaagat aataatatca agcctacttt ttcatattct tcaataccag ataatataaa
     5821 aaataaaatg attggtaaat ctatgcctac tgatgaacct ataagctttg atagtctatc
     5881 ttatttaaaa cttacatatt atggatttga tgaaaaaact catcaaggag aaatgatagt
     5941 aaacagtgaa ctagctccag aagtagtgga tatttttaag gaattatacg aaaaaaaata
     6001 tccaattgaa aaaattaagt taatagatga ctatgatgct gtagatgaaa aatctatgtc
     6061 tgataataat acctcatcat tttgttatag aactatagct ggtacaaatg tagtttcaaa
     6121 tcatggtaaa ggacgtgcta tagatataaa cccattacag aatcctcaag tttctggaaa
     6181 tgatgttaca cctaaagtaa gtacagttta tgctgatagg tctagcacaa aatttggaat
     6241 gataaaaaaa ggtgatgatt gttataatgc ttttgtaagc agaggttgga gttggggtgg
     6301 ctattggaaa aacccagatt atcaacattt tgaaaaataa tagattagaa ataaaggtga
     6361 attatatgaa tttaatgaca ttggaaaata taagtaaaag ctattctgaa aaaaaattac
     6421 tagaaaatat atctcttgga ataaatgaag gggaaaaaat aggacttatt ggtgtaaatg
     6481 gtactggtaa atctacctta ctcaaaatta ttgcaggagt tgaagaaagt gagactggta
     6541 atataacaaa aactaatggt attaggatag aatatctacc tcaaaatcca acttacaatg
     6601 aaaattcaac tgtacttgaa caagtattta atggaacttc tgaagaatta aagctacttg
     6661 gtgaatatca agaaatccta gaaaaactta attcaaactt tacagaggaa ttaaataaaa
     6721 aattattatc agtacacgaa aaaatagatg ccttaaatct ttgggattta gaaagtgaag
     6781 ctaagtcagt acttacaaag cttggtataa atgactttaa tcaaaaaata gcagaacttt
     6841 ctggtgggca aagaaagaga gtctctctgg catcagccct tattacacct tgtgagtttt
     6901 tgatactaga tgagcctaca aaccatctag acaatgatac tatagattgg cttgaggaat
     6961 atcttaattc cagaaaaggc tcattgctta tgataactca tgatagatac tttttagacc
     7021 gtgttacaaa tagaattatt gaactggaca aaggtagatt attcagttat gatgggaatt
     7081 attcaatatt tttggaaaag aaaatggaaa gactttcgct agaagcaagt attgaagata
     7141 aaagacaaaa tttaataaga acagagcttg cgtgggtaag acgaggagct caggctcgta
     7201 gcactaaaca aaaagctcgt ttacaaagat ttgatgaact tataaataga gattctttta
     7261 aacctgatga aaatcttgat atatctgttg catcaagtag acttggaaat aaaataatag
     7321 aaatacacaa tatatctaaa tcatttgaag aaaatactgt tatagataat ttagaataca
     7381 ctttaactcg tacagataga attggtataa taggcaaaaa tggtatgggt aaatctaccc
     7441 ttataaatat acttaatgga gaaatagagc ctgatagtgg atatatttca attggagaaa
     7501 cggttaaaat aggttgtttt tctcaagacg actctcatat gaatattgat atgagggcta
     7561 tagactatgt aaaagaagct agtgactata tagaaacttc agatggtact aggattacag
     7621 cttctcaaat gtgtgaaaga ttccttttca atggtactat gcaatatact ttgataggta
     7681 aattatctgg tggtgaaaga agaagactac atcttcttaa aatccttatg acagctccta
     7741 atgtgcttct tttagatgaa cctacaaatg atttagatat tgaaacgctt aaaatattag
     7801 aagaatacct agacgaattt aaaggtgtag tagttacagt atcacatgat agatatttct
     7861 tagatagaat ttgtaataaa atatttgcat atgaaggtaa tggaaaaatt catatctata
     7921 caggtaatta cagtgattat cttatttata gagagattca aggaattgaa tttgaagaat
     7981 ctaaaaagga atcagttaaa aaagatgact ctactaccct aaaaaaagaa aaacctaaaa
     8041 atgataaaaa gcttaagttc tcatataatg aacaaaggga gtttgaaaat attgactctg
     8101 atatagaaaa actcgaatct aaaattgcaa cacttgatga aagtactgca aaattttcaa
     8161 ctgattttac taaacttcaa gaaattttag atgagaaatc taaactagaa aaagaacttg
     8221 aatacaaata tgaaagatgg gaatatttaa ataatctagc tgatgaaata gctaataata
     8281 agtaatatta aaataatctt aagtttaata agttttgctt taaatctgta atttataaat
     8341 attttaaagg aagtgtctca aaatgaactt tttagtttta tgagacactc ttttttatga
     8401 gaaatcaaat atattttcat catttcaaca atacaaaaga ggcttatctc tattttgaga
     8461 cagcctcttt cataattacc ttctaattta tatgctctaa tatatctata ataattctta
     8521 ttatatctat atatccagtc tttcaatata atgacaatta acttcaatat gatttaaaaa
     8581 tactaacaac ctttcataaa gtggatgaga ttcttctcca tatttagctt ctaggttctt
     8641 gcctatttct ctaacagtcc tttgaccatc aatttgtaaa aaaacaaaac tggaatattc
     8701 atctagttct atttttttgt atttaggtat tctagctttt aactttctaa aaaacttttg
     8761 aattttatgg tcctgttttt caagtatagt aacaactcta ttttcagcta cttcatattc
     8821 aagtccatca gtaattttaa atacaatatc taaaacatca ttactcttgc tcattatttt
     8881 ttactttaga taaaacaata ggtaatgctg taagcacaac taaaactact aataaagcaa
     8941 ttgccattga atttgttgca gcaaatccac ttggtacctt tggagttaca actccagtta
     9001 cttgtaatat tatacctatt aaaccaatga tagaaccacc agcaacaaga ccagaagata
     9061 aacttatacc attagaaact ttaacttctt tttctttttc agttttagat actaattcaa
     9121 caaataaacg aattaatgct ccaactaata ttatagaagt tgttgcaatt ggtaaataga
     9181 atcctatagc tatagtcatt attggtaaat ttaatgcata tagaactatt gccataaata
     9241 ctccaacaat aatcataacc caaggtaaac tacctgacat aataccagaa gttaatgttg
     9301 acattaagtt tgcttgtggt aatgcaaatt gtacattatc accagttgct ctaagttggt
     9361 cagaaagtaa taaaattact cctacaacaa ctataacacc aactatactt gcaattgtaa
     9421 agtaacgttg catttcgttt ttgctaccac caataatata agttactttt tgagattgag
     9481 tataacctcc agctatagca atagcaacaa ctataaatga accaaataaa agtaatgatt
     9541 tattagcttc taaatcagtc catcccatta taacaaacac taatgttaca ataactagag
     9601 aagctatagt cataccagaa actggaagat ttgatgttcc gattgttcct gttaaacgac
     9661 cagctactat gacaaataat aaagataata aaagagatat gatagctcca ataattgcca
     9721 taacaatgtt accagaaatt aagaatgctg ctaaaaatcc aataactact ccacctaata
     9781 aaagtatcat ttgtatagat gaaccttctt ctccttctcc agcacttgat ttagctttaa
     9841 gtgtttcttt tatagaagcg attatagttg gtataagttt tattgcacca ataatacctc
     9901 cacaaagcat cataccagca cctatatatt ttacataact actagatatt gctccaacat
     9961 ccatttggtt aagaggcatt gcaggattat tccataccat agctccatct ttagccatat
    10021 ctgtaaagta tcctattaaa ggtgctatac caaagtttga taaaatactt cctgcaaaca
    10081 ttgttaaaga aacttcaagc cctactataa atccaattcc taaaagtaga ggatttactt
    10141 caatatcaaa tttccatttg tagaatttgc ttcctacaag actcattaca ttgtttgcta
    10201 catttaagaa tgaaccagtt aatacagtaa taaatccact tattacaaat ccaattccca
    10261 tgtacttaat tgaatctcca ccagcctctg aagcaacaag tgtttcagat atagccattg
    10321 attctgggta cataagttta ccatgttctt caactattag atagttgtga actatagcag
    10381 caattcctat accaaataaa actccaccaa ctccaacagc taagccttca aagaatgtaa
    10441 tttggcttcc aattaatata actgctggta atacgaaaat aaatccactt gcaacagatt
    10501 ctccaccact tgacatacct tggattaagt ttttaccaag aatacccttt gaacgagcaa
    10561 atgctccaac aaaagcagaa cctattatag ctcctggaat acctgcggca actgtaagac
    10621 cggctttcat tccagagtaa gttgtagatg cggcaaaaat ggcagctaaa ataataccga
    10681 taatcagtac agctacattt ccaccggttc tggacttatc tgtgatatat ggaacgtaat
    10741 cttttccact aacctcacca taagcaccct taggtaattt tttgttcata aaattttcac
    10801 cccttttcaa tttgtatata ttataacata tatacatata aaacttaact ttttattatt
    10861 ttacaaatag cgagtcaaaa ctactaaaat ttaaccctat attattaatt tctactttta
    10921 gatattttta tgctaatttt ctttttattt ttcaattcta ctaaaaacta ttaagatata
    10981 aaataaaaa
//
LOCUS       DBGJFU010000124        10981 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.125, whole genome shotgun sequence.
ACCESSION   DBGJFU010000124 DBGJFU010000000
VERSION     DBGJFU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.125"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            172..1335
                     /locus_tag="ACOIAS_003051"
     CDS             172..1335
                     /locus_tag="ACOIAS_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGZ2702742.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLSR
                     FIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIVEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            1384..2235
                     /gene="gatY"
                     /locus_tag="ACOIAS_003052"
     CDS             1384..2235
                     /gene="gatY"
                     /locus_tag="ACOIAS_003052"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGZ2702743.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            2561..4462
                     /locus_tag="ACOIAS_003053"
     CDS             2561..4462
                     /locus_tag="ACOIAS_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGZ2702744.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            4465..5508
                     /gene="ypdE"
                     /locus_tag="ACOIAS_003054"
     CDS             4465..5508
                     /gene="ypdE"
                     /locus_tag="ACOIAS_003054"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGZ2702745.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            5523..5828
                     /locus_tag="ACOIAS_003055"
     CDS             5523..5828
                     /locus_tag="ACOIAS_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2702746.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            5925..6260
                     /locus_tag="ACOIAS_003056"
     CDS             5925..6260
                     /locus_tag="ACOIAS_003056"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2702747.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            6292..7614
                     /locus_tag="ACOIAS_003057"
     CDS             6292..7614
                     /locus_tag="ACOIAS_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2702748.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            7636..8694
                     /locus_tag="ACOIAS_003058"
     CDS             7636..8694
                     /locus_tag="ACOIAS_003058"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HGZ2702749.1"
                     /translation="MERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVTKDKN
                     YQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSIQEYL
                     SLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMTELEV
                     SALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVYQGYQ
                     SDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKHGFGE
                     YFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDIVIID
                     GKGVPLNKTSKEFIILGE"
     gene            8699..9886
                     /locus_tag="ACOIAS_003059"
     CDS             8699..9886
                     /locus_tag="ACOIAS_003059"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2702750.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(10083..10955)
                     /locus_tag="ACOIAS_003060"
     CDS             complement(10083..10955)
                     /locus_tag="ACOIAS_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HGZ2702751.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
BASE COUNT         4313 a         1196 c         1937 g         3535 t
ORIGIN      
        1 aaagataaac tataaaatta attaaaatct atagtgtata ttttatagtg agtttaagta
       61 gtactaaaaa tctaagcaaa aaattataaa agagtatatt tttttatagt ttaaagtggt
      121 tataacctca ttaccaaatg taaacgttac aagtatgaaa ggagaatgaa aatgtttaaa
      181 ttagaagaac aaaaactaaa agatttaggt gcaataataa caactaatga aataaaacaa
      241 cagcctgaat tatggttaga gacttatgaa atatataaat ctaacaaaga aaaattaagt
      301 agattcatag atacaataag taataatcat gggcagttta gagttatatt tacaggtgct
      361 ggaacttcag cttacatagg aaattcaata cttccttacc ttaaaaataa aaatgacata
      421 agaaaataca tatttgaagc aatacctact acggatatag tttcaaaccc atatgattac
      481 ttaaaaaaag atataccaac attattaatt tcatttgcaa gaagtggaaa tagtcctgaa
      541 agtttggctg ctttaaattt agggaataag attgtagata atttttatca tttagctata
      601 acttgtaatc cagaaggaga actagctaag atgactaaaa acgacgaaaa taattacttg
      661 ttattaatgc catcaaaatc aaatgatgaa ggctttgcca tgacaggaag tttttcgtgt
      721 atgatgttat ctgctatgtt aatattcgat agtttagaag atgatgtaga aaagtcatat
      781 ataaatgcaa tagttgaaat gggaagaaat gttatagata gaaaagatga aatacatgag
      841 ttaataaata aagattttga tagagttgta tatttaggtt caggaggatt aggtggtttg
      901 acacaagaag cacaactaaa acttttagaa cttacagcag gaaaaatatc tacagtgtat
      961 gattctccta tggggtttag acacggacca aaatcattta ttgatgaaaa tacacttgta
     1021 tttgaatttg tatctaactg tttatataca agaaaatatg atttagatgt tttagaggag
     1081 ataaaaaggg ataaaattgc taaattcact tgtgcagtgt cagtagaaaa tgaaaataac
     1141 ttctcaggaa caaagtttga gtttaaagaa aagtataata aattaccaga tgtgtatctt
     1201 gcaatgccat acatattgtt tgcacaaact atagcattgt ttgtatcggt aaaggttgga
     1261 aataagccag atacgccatc agctacagga acagtaaatc gagtagtaaa aggtgtaaca
     1321 atatatgaat attaaaagat aagattaaaa tattataact aaataatttg ggagggtatc
     1381 aatgtggcat tagtaacaac aacatcaatg cttaaaaagg cacaagatga agggtatgca
     1441 gtaggagcat ttaatgtaga aaatatggaa atggtaattg ctgttataga agctgctgag
     1501 gagttaaatt ctccagtaat attacaaact actccatcta ctgtaaaata tgcaggtctt
     1561 gattattatc tagcaaatgc aaaggttgct gcagaaagag taaagatacc tgtagcaatg
     1621 catctggacc atggttcaag ttttgacctt gcaatgaaag ctttaagaag tggatataca
     1681 tcaataatga tagatggttc tcatgaaagc tttgaaaata atatatctat atctaaatca
     1741 gtagtaaaag catgtagtcc ttctcttata ccagtggaag cagaacttgg gaaagttggt
     1801 ggaaaagaag atgatttaga tggcgggaat agtaatgatt atacaaatcc aaaagaggca
     1861 aaagagtttg tcgatagaac aggaatatct tcattggcta ttgctatagg tactgcacat
     1921 ggattgtata agggtgaacc taaaatagat ttagataggt tatcagaaat taaaaatgta
     1981 gtttcaatac ctttagtatt gcatggaggt tcaggaatac atgatgatat tataaaagaa
     2041 gctataaaaa gaggtattgc aaaagttaac tatgcaactg aattaagaat tgcatatagt
     2101 aaaggggtta aaaaagtttt agatgaagat tctgaggtta tagaccctaa aaagtatgga
     2161 ttagcagggc ttcaatcagt aaaggatttt gtgaaagaaa agattaaagt gtgtgggtct
     2221 gtaaatagag tataaaaata aaatatccta aatttatttt agataagttt aggatatttg
     2281 ttatatgaaa aatttatatt aattatatgt aaatggtgta tttttttcat ttaattgatg
     2341 aaaaatatca gcttttattg gtaaagtatt acatatataa ttaaaatata ctaaaaattg
     2401 aactatatat tttattatta taaaatattt atattaaatt gtggaattaa aatatactag
     2461 gatataaaat tcttaaataa aaagaaaaga ttgtttttat aaaaaatatt ttttatacca
     2521 cttaaaaaaa cacaataata acactgaggg aggtggggat atgagagaac gcattattct
     2581 tgaaacatta aaaaatagta gtggttatct aaacatagat tattttgcag ataagttggg
     2641 ggtatctact cgaactatca gaaatgaaat aaaaaagata gaaactataa aagaatgtaa
     2701 tggttttaaa ttagaatata agtctaaatt aggatatatt ctaaatatta aagaccaaga
     2761 taaattcgag cattatctaa gaaacttacc ctcagattta gttgaaaatc cagaacaacg
     2821 gttagagtct ataatagtag aacttttagt aaatgaaggt tataaaacta tagaacagtt
     2881 aagtaaaaaa tttctagtaa gttcatccca aataaaaaat gatttaaaaa agatagatga
     2941 aaagataaag gatacagaat taaaactaga gcgaaaggct cattatggaa ttaaaataga
     3001 aggttctata aaagaaattc aaagtatatt ggtggatagt tattttagag ggaatagaaa
     3061 tataacagag tacaggaata agtttataga taatttaaaa ttagctaata ttagaagtac
     3121 aataaaaaat gtacttaatg aacatgattt agaagcaaat ttaacagaac ttgaggaaat
     3181 attagcacaa attattatat tgtatataag agttagtatg agagtagcgg gaaatgctag
     3241 cgatttaaaa ttatatacag aagatttaat tatagataaa ttattagata aaatttttag
     3301 agataaaaaa tataacctta attatgatga aaagtattat ttaaaggagc ttataaaact
     3361 aaagactaaa gataagaaag ccactataaa gaatattgat aagaataaac ttcaagatat
     3421 tatgttcgaa ttttttagag agatagataa aaaatataac tctaattttt tagaagataa
     3481 agagttcttt aacttatttt acttacatat tgcatgttta atagaacgta taaagaagaa
     3541 ccacaagata ataaatcctt tttcagttaa aataagtcaa cagtatccaa ctgtttttaa
     3601 tttatctatt caattttcaa aaatcataga aaatgagtat catataaaaa taagtcaaga
     3661 tgaaataggg tttatagcta ctcacattgc tgttcctttt gaaaagagag aagaagctaa
     3721 ttttaataaa aaatataaaa tagcaataat atgctcttct ggtggaggta gtgcattttt
     3781 gataaattta agacttaaag aaatttttcc aaatgctgag ataagaaatt tttcattact
     3841 agaagaaaaa gctgtaatag aatttgcacc agatttgatt ttttcaataa caaatctttt
     3901 atttgaaata aatgctccag ttatacttat aaatgaaatt ttagacgaat tggattattt
     3961 agatattaaa gaaagtgtta gatttgcaga ccatatagga aatatatcaa gcccaaaaca
     4021 atatatatta ggtctatttg ataagaatca ctttaggtgt ataaaagata aagctaagta
     4081 taaggatatc cttgatagta tgtcaaaatg tatagttgac gagggcgctt gttctccaac
     4141 ctatcccaaa gatgtatggg aaagagaaag ctatttgagt actatttata caaatggggt
     4201 ggctatacct catccaatcg agatgacagg aaacaaaaat ataatatcag tagctttgat
     4261 acaaactgat attatttatg aaaatagggt gcctaaaatt attttcatga tttcattaat
     4321 aaagggaaat ttagaacttc ataagcaaat atctaaatat ttaagtaaga ttatggcaaa
     4381 taaggatatg gtagacatgc taaataagtc acaatcttat gaagagttta tgtataagtt
     4441 aaaaatctat ctgggaggtt agacatgaat atagagtttt ttaagaggtt gtcagaagca
     4501 gatggtatag catctaatga agaagaggtt agacatgttt tattggaaga actaaaagct
     4561 tatagtgata aaattatctg tgatgggcta gggagtatca tcttttctaa aataaaagat
     4621 gagagtgcac caaacgtaat gatttgtgct catatggatg aagttggatt tatggtgaga
     4681 agtatagaca aacttggtat gattcactta ataacaatag gaggagtaaa acctcttgca
     4741 cagtttgtac aaaaagttag aattacaaca aaagaaggga agaaaatccc tgctgtcata
     4801 aatgctacat ataataatgg taaggctgaa aatatatatg cagatattgg agcatataca
     4861 gaagaagatg tatataacct agggattaat gttggggata tggtaacata tacaacaagc
     4921 tttgaggaat ttactttacc agaccgatta gtgggaaagg cttttgatga taggatagga
     4981 tgttttgtga tgggagaagt tttaaaagaa cttaggaaag aaaatctaaa ttgtaatatt
     5041 cactttgcgg ctacaagtag tgaagaggtt ggaataaggg gagcaaagac atcaacacag
     5101 ttaataaatc cagatatagt atttgtaata gatgtagcat gtgctaaaaa tgagtttgtg
     5161 agagactata caaatcagaa acaaattgat aaaggtgtaa tgctaatgca tagagatagg
     5221 actctagtcc ctagtagaaa aatgatagat tatgtaatgg aaattgctga taaaaataat
     5281 ataccacttc aacatgatat gtttgaatca ggtgggactg atggaggaga agcacatctc
     5341 gtaaatgaag ggaaaccttg tgtagtaact tgtgtaccag ttagatatgg tcattgtgca
     5401 ttttcaatag taagtaataa agatttagag aacattataa gattgtatac acagttaatt
     5461 ttaaattttg atgaagaaca atataaacat ataagaaatt ttatctaaag gagaataaga
     5521 aaatgtcaac agaagtacaa ataatggaac ttatatcaac tgcaggagag agtaaatcga
     5581 aagcttttga ggctttaaaa aaagtgaagt caaaagattt taaaggagca aaattgttaa
     5641 tagaggaggc tcgtaaaata gatattgaag ctcacaggat acaaacagaa ttaataactc
     5701 aagatttaaa tgaagatgtt gaaagtccag caatgagtct tttaatggta catgctcaag
     5761 accactatat gacttctcag ctagcaaaag acttgataga ggctttgata tattgtcttg
     5821 ataagtaatt gtaatataga aatagattat ttatgtaaaa attcttttaa aagtttagat
     5881 gcttttaaga gataataaaa atacaaactt taggaggaat tattatgaaa atattattat
     5941 gttgtgcagg tggattttct actaatatgt taatgcaaaa tatgaaaaag gtggtaaaag
     6001 agagtgctaa actcaatgaa aaagattttg agtttacagc tataccagca gattcaatag
     6061 aaagtgaagt tgatgattgg gatatagtac ttataggtcc tcaaatagct cacaaaatag
     6121 acttcttaaa aggaatatta gagccaaaaa ataagccttt tgcagtaata gataaagatg
     6181 tatatggaaa aatggatgga gctacagtta tgaagttggc attaataact tataagaaac
     6241 atcaattagc tcaaggataa taaaaataat gagggaaaag ggagattaga tatgttttca
     6301 aaatttgaag catttatgaa tagagtattt atgcctttag ctcataaggt tgacaatcaa
     6361 cgtcaccttg gttctataaa ggctggtatg gttgctatga caccattcac aatattagga
     6421 agtatatttg caatacttcc agcacttcca aatatgcttg gagaaaataa tccaataagc
     6481 aaattcatac ttaacaattc agcaatattg gatttaccag taagtctttc cataggttta
     6541 ataggattat atgcttgtat gtgtattgct tttaatctgg ggaactacta taaattatat
     6601 ataccaggtt gtgttgcact atcaactttt tcttttttat tcctagtagc atcatttaca
     6661 ccagatggag ctttagaaat aggaaattta ggagctaaag ggctatttac aggtatgata
     6721 gcagggcttg gttctgtgga gttatataat ttttgtaata agaagaatat cacaataaaa
     6781 atgccagaaa atgtacctga ttttgtatct aggtcttttg aattaatacc tactactata
     6841 atagtttgtg gaacttttat agttgctcgt tttatttcat taaaagtatt tggagaatta
     6901 cctccacaga tacttactca atttttatct ccattagtag gaagtatgga taacccatgg
     6961 tcagttcttg cacttaactt tgctatatgt actatattct tttttggaat acattcatca
     7021 gtatttagtc ctataactag acctataatg attacattta tagctgaaaa catagcagct
     7081 atgcaagctg gagaaacaat acctcatttc tttacagcag gagcttcaag tgcattcttt
     7141 ggatttacag gatgtggaat tagtataggt tgtgtaattg catgtatgtt atcaaaaaat
     7201 gcttcctata agaagattgg acgtgtatca ttattccctg cattatttgg gataaatgaa
     7261 ccaatattat ttggagcacc gataatatta aatccaataa tgtttatacc acatgtattt
     7321 ggtggagcta taataggtac actaccaatg ttttttatgc actggggatg gttagcaaag
     7381 ccagtatttg accctccata tgtaggggta ttcttagaag gatttttaac taatggagat
     7441 tggcattcta tattagcaaa tgcattgcaa ttagtattag cagttttatt atattggcca
     7501 ttctttaaaa taatggaacg tcaacaaaat aataaaaaac aaaaagaaga aactaagaat
     7561 atattctcta aagaagaaga agacttattg gatgatttag gattggattt ctaatatttt
     7621 gttatgaata atacaatgga gagattagta tccttaatct ataaaagagg tgttgacgct
     7681 cttttgatta aagcaaaaaa tactaaaaga tatataggag ctttatcagg tagtggtgta
     7741 tatgtgcttg ttactaagga taaaaattat caaattctag atggcagata tattgatgaa
     7801 gcagataaaa aaacgactgg tttcataaaa aggattgtat ctcaaggttc ttatatacca
     7861 actataattg agttattaaa agaattaaat ataaaaaagt tagcaataga aaaccaagct
     7921 atgagtatac aagagtatct ttcactaaaa aatgaaggtt ttgaaatcaa tttaataaca
     7981 aatgaattat ggaagctaag ggctataaaa tctaaagaag aaattgaact tattaaaaag
     8041 gcttgtgaaa ttacagatga agtatttgat gaggttattt ctgaaattaa aaaagatatg
     8101 actgagctag aagtatcagc tctaattcag tatcatgcat taaaaaaagg tgcaagtgga
     8161 atgtcttttg aaacaatagt ggtatcagga gaaagaggtg caatgccaca tggaagacct
     8221 acaaacaaaa aattgaaagt gaatgaagct ataacaatag attttggtgt tgtttatcaa
     8281 gggtatcaat cagacatgac tcgtacaata tctataggta aacctcctaa aattattaaa
     8341 gagatatatg atgtagtatt ggaagcacaa ttatctgcaa tagagtcaat aaaggaagga
     8401 actagggcaa gtgatgttga caaagtggct agaaaaatta tagataaaca tggatttggc
     8461 gaatatttta atcatggact gggtcatggt ataggtcttg gagatggtga agtccccact
     8521 ttaaatccaa atagtgaaga tatattagtg gaaggaatgg taatgtcttg tgagccaggt
     8581 atttatattc caaatgtagg gggagttaga atagaagatg acattgttat aatagatgga
     8641 aaaggtgttc cactcaataa gacatccaaa gagtttataa tattaggaga gtaaaattat
     8701 gaaatataat tttaataaag tattaaatcg taaagaagga aattgtagaa aatggagcaa
     8761 ccatgtgatt aaagaaaaat tcggcttaaa tgaagatgca attcctatgg atttagctga
     8821 tatcgatttt gagtgtgctc ctgctataaa agaatctatc ttaaaaagag catcagtagg
     8881 agattatagt tatactttta taggagatga cttctatgat gcgattataa attggaataa
     8941 aagaagattt gatgtatata tagaaaagga ttggataaaa ttaacttttg gtactgtttc
     9001 aacccttcat tatatagtcc aagcatatac taaagaagga gagggtgtat tgataaatac
     9061 acctgcttat gacccttttg cagaggctgt agtaaataat aatagaaagt tgtgttgtag
     9121 ctcattaaaa ttagataaca atagatatta tttagattta gaggatatgg aaaatcaaat
     9181 gaaatacgag aatataagat tgcttatatt ctgtagtcca caaaatccat caggtcgtat
     9241 atggacaaaa gaagaactat atcaagtgtc agagttatgt ttaaagtata atgtaatttt
     9301 ggtaagtgat gaaattcata gagatataat atttaaagat tatagatttg tatctttatg
     9361 gaatgctcat ccagaaattt ataaaaatag tataatctgt gtatctccta ataaaggctt
     9421 caacttaggt ggattaaaaa catcatatgt attaataaga gatgtaaaaa ttagggagac
     9481 tttattagaa agactaaaaa gtaattcaat aacttctcca aatgtatttg caataccagc
     9541 aatagttgca gcatataatg aaagcgaaga atggctagat gctatgacta gttatgtaga
     9601 ggagaatttt gaaatagttt atgatttctt tgaaactaat ataccaaaag ctaaagtgat
     9661 gaagtcagac tcttcttttt tagcctggat agatgtaaga gaagtattta gaaatgaaga
     9721 agaatctaaa gaatttttta gacatgcaaa cttaactatg gtagttggaa gttattttgt
     9781 gaaagatgga gatggattta ttcgtattaa tgtggggtgt cctagagaaa cattaaatga
     9841 ggctctaaat agaatcaaaa aaacgtatat aagtatgtat tgttaaatca taaaaaaagt
     9901 atctatggtt tatataaaaa cacattattt agttatcaaa tattactgga attttgtatt
     9961 ccagtaatat tttttgatta aggaatatta ttgaggaata ttaaataata ttatttcata
    10021 cccttatatt aacttatata aaaatatttt attattttgg gaacgggtta gctaaccttt
    10081 atctagtgta tatttatatt gtaagaagca gtaaaaactt ttcctttact aagtttatta
    10141 ataaacttct tatctttata aattttgtta gaatttatac tatcagcaag accataccaa
    10201 ggttctatac ataaaaaagg agcagtagaa ttagtttcac tataataagg agtccaaata
    10261 ccaactagag ggaaatcttc catagatata gttacatatt tactaccatc attattacat
    10321 atagatactt catctatgtt ggtgtatatc aaagcatcat tcttgaatac ttctggattt
    10381 aactgtaagt tttttaattt tccaataggt attatatcat ctgtaaaaga accatttaaa
    10441 ttgatttttt ctacatcatt tttactttta aactcaaggt agtgtttaga aaaatcattt
    10501 tgttcgtaaa aaggaaggtt gaatgcagga tgtccaccta tagaaaagaa tatatcttta
    10561 gaatcagtat tttttacaat ccattctacc tcaatactat tatcatttaa tgtatagttt
    10621 atgattagct caaaagaata tggatacttc tttaaagtag attcattatc tgttaattta
    10681 taagttacaa atgtatctcc tttatctaca atctcaaagt ccatatctct agcaaaccca
    10741 tgttgagtca tgctatataa attttcttct attattgttt cattatcaaa aagtttacct
    10801 actataggga acaatatagg tgattgtcta ccccaatatt ttttattacc atcccaaaga
    10861 atgtttttgt cgtatttttt agaaaatatt ttagttagtt cagcaccaaa actgcttgat
    10921 tcgataatta aattttcatt ttgtaatata ttcatttaaa atctccttta ttatatttta
    10981 t
//
LOCUS       DBGJFU010000125        10524 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.126, whole genome shotgun sequence.
ACCESSION   DBGJFU010000125 DBGJFU010000000
VERSION     DBGJFU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.126"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            270..1433
                     /locus_tag="ACOIAS_003061"
     CDS             270..1433
                     /locus_tag="ACOIAS_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2702752.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGPDQSIDPIKPACDIVLKVSSIPVN
                     HISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMSNGVG
                     QVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGEILKE
                     YSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDILKLGCEFMAKYAVEFLNKNNN"
     gene            1467..3005
                     /locus_tag="ACOIAS_003062"
     CDS             1467..3005
                     /locus_tag="ACOIAS_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HGZ2702753.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            3037..4239
                     /locus_tag="ACOIAS_003063"
     CDS             3037..4239
                     /locus_tag="ACOIAS_003063"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGZ2702754.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(4406..5005)
                     /locus_tag="ACOIAS_003064"
     CDS             complement(4406..5005)
                     /locus_tag="ACOIAS_003064"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702755.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            5175..6533
                     /locus_tag="ACOIAS_003065"
     CDS             5175..6533
                     /locus_tag="ACOIAS_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702756.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            6526..7158
                     /locus_tag="ACOIAS_003066"
     CDS             6526..7158
                     /locus_tag="ACOIAS_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGZ2702757.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            7487..8644
                     /locus_tag="ACOIAS_003067"
     CDS             7487..8644
                     /locus_tag="ACOIAS_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HGZ2702758.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTLDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(8791..9426)
                     /gene="lexA"
                     /locus_tag="ACOIAS_003068"
     CDS             complement(8791..9426)
                     /gene="lexA"
                     /locus_tag="ACOIAS_003068"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGZ2702759.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(9530..>10524)
                     /locus_tag="ACOIAS_003069"
     CDS             complement(9530..>10524)
                     /locus_tag="ACOIAS_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGZ2702760.1"
                     /translation="FIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITE
                     DDFNTIKSRDINLFLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNS
                     QIDNNITDGFNPIKLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLR
                     DKAILALFVTYGLRLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDY
                     LQNERTRDELLNDEEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKL
                     RATAATSLIQTGFSIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         3916 a         1310 c         1731 g         3567 t
ORIGIN      
        1 tttaaccact aaactttttt aatgtctata atctactatt atttaattta ctaaaaataa
       61 aaaaactcat tatattaaga tagaaataga aaaataacta gaaaaatact ataatatatc
      121 agcttaatac agaaaaaagt taaaaaattt gagaaaaata aaaatgaaaa cgttttatat
      181 tgacatagag aaaaagtagt gataccattt aaatatagtt tagtagctaa actatattta
      241 aattataata actattttag ggggatgtta tgaatttaaa acaattaagt gaaaaatacg
      301 aagaatatgt aattcaaatg agaagagaat ttcataagta tccagaatta tcttttaaag
      361 aaataagaac aacccaaaga ataagggaag aattacaaaa aataggggtc ccttttgtat
      421 ctattgaacc aaatatcgta atagctacta tacaaggcaa aaatgaaggt aagacaattg
      481 caattagagg agatatagat gcgcttccaa tgcaagaaga ttcagatgtt gaatacaaat
      541 cagaatatga tggaataatg cattcttgtg gacatgatgc tcatggagct atgttactag
      601 gacttgcaca tatgctaaat gatataagaa aagatttaaa aggtagagta ttattagttt
      661 ttcaagcagc tgaagaagtt ggtggtggac acaaggagat aatacagtac tttaaggaaa
      721 atggaggacc ggatagatta atagctactc atgtttggtc cgatattgaa gctggtaaaa
      781 tttctgttga accaggacct cgtatggctg gtgggtctgg ttggagaata gacataacag
      841 gtagaggagg tccagatcaa agcatagatc ctataaagcc agcttgtgat atagttttaa
      901 aagtatcttc tataccagtt aatcatataa gtgtactaga acaatgtgtt gtacatgcat
      961 gtgcaataca tggaggaact acaataggaa atgttattcc aaacaatgca gaggtaatag
     1021 gtggatatag atattttact gaagaatcag gcaaaaaagt gtttgacatt ataaagcaaa
     1081 tgagtaatgg tgttggacaa gtttatggag tggatgtgaa ggttactcat actggaggca
     1141 taggacctgt tatcaatgat gaagaagcag ttaagatggc taaagaaata gtttctaata
     1201 tagatggatt ggagttagat tcttatgagc caatatgtgc atcagattgt tatggtgaaa
     1261 tattaaaaga gtattctggc ttttattgtt atctaggggt aggtaacaaa gaaaaaggaa
     1321 taatatatgc acaacaccat cctaagtaca atatagatga agatatatta aagttaggtt
     1381 gcgaatttat ggctaaatat gcagttgaat ttttaaataa aaataataac taaaataaat
     1441 ctaattttat aaatgggggg atgaaaatgc aaggtgcatt aaaggaaaat aagaaaaaaa
     1501 gtggtggagg atttttagga tggattgaaa aagttgggaa taaaatgcca catcctctag
     1561 cattatttac atatattaca ctgatagtag tagcattatc tgcaatagca agctttttag
     1621 gtttttcagc aacaagtcct gctacaggaa agttaataaa agttatgaac ttaataagtg
     1681 caaaaggttt agtactattt atgcaagagt ttgttaagaa cttccaaaac ttccctgtgc
     1741 ttggagtagt tttagttatg gcagttgcca caggagtttg tgaaaagaca ggattttttt
     1801 caactgccat aaaaatgagt ttatcaaaag ttaaggggaa tgcggttgta tttataatag
     1861 cttttatagg agtatttgct aatcaagctg gtgatgctgc ttttgtatta gtaccaactc
     1921 ttgcaggagc aatattttat ggattaaata gaaatccatt agctggtata ttctgtggat
     1981 ttgcatcagt tggtggtgga tttgcaacag ctgtaattcc tgggggatgg gacgttacgc
     2041 taacgccaat aactgtatca gctgctcaaa ctattcaacc tgcatttgat atgacattat
     2101 tagctagtta ttatgcacta tttgtatctg cgtttatagt agctacagta tcaacaatag
     2161 taacggttaa atttatagaa cctaaattag gtaagtatac tggtaaacca gaaggtatag
     2221 atgataatca aggttttgag gtcacaaaag aacaagcaaa agcagcaaag ttagcaggtt
     2281 ttacagtttt agcttatgta gcgttattaa ttgctttgtg tataccagct aatagttttt
     2341 taagaagtcc agaaggttct ttaatatttg gagcaccact tatgtcttgc ttacagtttt
     2401 ttatagtaat actattcttc ttgccaggga ttgtgtatgg aatgagggtt ggtaaaatca
     2461 agactgtaaa agacttaaat gatatattat gtcaatcaat ggcagcaatg gcaccgttta
     2521 tcgtacttgc aatagttata ggtcaattct taacgctatt ctcagtgtct aatttagcac
     2581 aggtactagc tataaaaggt ggagcattac tcaactcatt gccactacca ccacaagcta
     2641 taatactgct attcttagta ctagtagcgt ttataaatat atttgttata agcggaagta
     2701 caaaatggat gatatttgga ccagtatttg ttccaatgtt aatgcaatta aacatacatc
     2761 cagcatttac tcagtttgta tatagattag gagattctgt aaccaatcat ttatcaccat
     2821 taaatgcatt ctttataata cttttagcaa cagtccaaaa atacgataaa aatgcaggta
     2881 tgggaactat attctcagct atgatacctt acactatagg ttactttgta gtacttgcag
     2941 ttttagtagt agtttggatg acaataggta taccagtagg aataggtgga caagtttggt
     3001 tataaaacat atttaataat tttgggagga aaaaaaatgg aaacaactga aataagatac
     3061 aattttgatg aagtaataga tagaagtgga acaaactgtc ttaaatatga tcttaagagt
     3121 caatttatgc cagaatctcc agaagatgca ctaccactat gggtagcaga tatggatttt
     3181 gcatgtgccc ctgagataat taatgcaatg catgaaagaa tagacagaaa aatatttgga
     3241 tatagctctc aacaaactga tgattattat gaagctgtat gtggatggtt taagagaaga
     3301 tttgattgga aaatagaaaa agagaatata ttctttagcc caggggttgt gcctgcgtta
     3361 ggatttttca cacaaatatt aacacaaaag ggtgatggta tcataataga aaagcctgta
     3421 tactatcctt ttgaagataa aataactaac aatgaaagaa aagtagttaa tagtccttta
     3481 aagtttgaag atggtcatta ttctatggat tatgatggat ttgaagaatt agctaaagat
     3541 gaaaataata aggtgtttat attatgtagt cctcataatc cagttggtag agtatggaaa
     3601 aaagaagagc ttagaaaaat agtagacata tgtaagaaat atgatttgtg gataatatct
     3661 gatgaaatac attgtgatat tattcgtaaa ggtgaaaagc atataccatt agaagtagca
     3721 tgtcctgatt ataaagatag aataataact tgtacagctc caagtaagag ttttaactta
     3781 gctggtatgc aattatcaaa tatagtaatc aataataagg aattacaaga aaaatggatg
     3841 cttgaaacag attctaaatt agctatattc ctacctaatc cattcgcaat agttgctact
     3901 aaagttgctt ataatgattg tgaagattgg atgaatcaag taaatgagta tatagatggt
     3961 aatattaaat atgtagaaga ttttgttaat gagtatatgc ctaaagtaaa gataataaaa
     4021 tctcaaggaa cttacttagt ttggttagac tttagagggt atggattaga tgcaaaacag
     4081 cttgaatatg taatgcttaa caaggcgaag gtattgctag atgaaggata catattcgga
     4141 gaagaaggag caggatttga aagaataaat gttgcttcac ctagaacaat tatagaagag
     4201 tgtatgaata gaataaaatg tgcatttgaa ggattataat agtaatatct aaataatata
     4261 tttgtgtatt ttaaaaaaag actatgctaa aaaaatattt caaagcatag tcttttaaag
     4321 ttttatgatg agtttaaaaa atttcaaaac gttactattc acttatatgt ttatattaga
     4381 aataattcca caatatactc attttctaaa gttgcacttt tatacacctt gcttcaccaa
     4441 tatcatgtgt aaccaaaata gtagacttat tctcattctt aataatacta taaacatcat
     4501 cacaaactaa taatctagtc tgataatcaa gagctgaaaa aggttcatct aaaagtagaa
     4561 tatctggatt tacagaaagt gttctaataa gcgcaactct ttgtctcata cctccagata
     4621 attcttttgg atacatactt ctaaaatccc ataatccata agtctttaaa aggctttcaa
     4681 ctctatctaa agatttttta tcttttttgc cttgaatttc caatccaata gttatattat
     4741 ccattatatt tctccattca agcaaattat ctttttgaaa catatatcca atatttccag
     4801 taatctttat atcaccacta gttggtttta aaagattagt aagaatattt aatattgtag
     4861 atttaccact tccagaagga ccaagcaaag ttaatatttc tccttctttc aagttaaaat
     4921 tgacatcctt taatacttca agctcgcctt ccttagtgta gaagttcata tttatatttt
     4981 taacttctac tatgttgtca ttcattatgc caccccctat aacttttagc tcgtatatat
     5041 tatgttatta acaatatgac ctatttgtac catttttcat aaatataata taaatatttt
     5101 gtttatttac gtattttctt agtaacttgt acgtataaat tatagataaa taaattataa
     5161 ggtaggggat aaatttgagt aacataataa cgaatttaat agagtcaata tttactaatc
     5221 caataactat attgttttta cttgcaataa ctgtgtattc tgtttttaaa ataatagaaa
     5281 ataacagagt atattcattt gtaagttcaa aattagacga aatcaataga gactataaga
     5341 caagtgagtt ttataaaaag gtaagggatg actactatac atatagtaaa gaaaatcctt
     5401 atgcagacgt aaatataact tcatttatag aggaagttgt atctgattta aagcataata
     5461 atttgccatt gttagaaaaa attagaagta taaaaaattc atcttcaata agtatattac
     5521 ttggggtttt aggtacgttt gttggtcttt ctacaatgct tttatgtgtt gatactaaag
     5581 acattataaa ttcacttcct tcaactatta gtagtatgca aactgcattt acaactagta
     5641 tatttggagt tgtattttct ctaattatag gttattttac taagataaaa gattgtgaac
     5701 atgttttgat acaaataatg ttaaaatctg aaaatttact aacatctgaa ataactcatt
     5761 ttaagtcaga aagaatggat ttaaaagtag aagaggtaaa aaatacaata aaacaaataa
     5821 gtaagtctat agaagctatt gaaaaatttg ataaaatatc taaagattta aatgacttta
     5881 atgatgaatt tatcagtggt atagaagctt taaaaagcct tttagaaggt tctcaaagtt
     5941 ctattaagac atttgaccag agcgtaagaa aattagataa acagtttaat attttaaata
     6001 taaaatttgt aaagcttttt gataaatatg ataatcaaga taatataaat aaagagattt
     6061 tatttgatat aaaagaatct tcaaaaaata tatacaatgc cactgaaagc caattcaaaa
     6121 ttagagatta tataaaaaat ataaatgcag gatttgctct atatgaaaga agtgctcaag
     6181 atttattaac taaattaatg acccatgaaa ataaaatctc tcaaaatcaa aaaatccttt
     6241 tagatgaaaa gtttacctta gatgactcaa taaaaaatct ctcaagtatt attgaaaact
     6301 tttctaatga cctacaagtt aaactggata tgatgtttga aaattcttta gatattcaag
     6361 ataagttaga tgttatgttt aataactctt ttatgaatga tgaagttcct cttgatagtg
     6421 aagaattatt taaggatgat gttaataatg tatttaatcc ttttagtgaa gaaatttatg
     6481 agatagagga taaagaaata aaggtcatag gtgaggatga gttaaatgaa taataaatat
     6541 agaagaactg ttaacagaga atttgaaaag agtagctttt ggccaacatt tacagattta
     6601 ttatctactg tattgatggt tgtaattctt atcttattta gctcagaaag tatttcaggt
     6661 tctgttgaac aagatttagc taagaatgtc aatgcttctg ttgaagaaac ttttaaaaaa
     6721 agtggaatac ctgtgaaggt agataaatct aatgggcaag taacttttgg tgaaaaaacc
     6781 atgtttgatg tagatagtga tgttttaaaa ccagaggcta aagaaatgct aaaaatgttt
     6841 gttccaaagt acatagagac tatttataaa gattatggtg actatatatc caaaatagtt
     6901 atagaaggtc atacagatga tgtgggaagt tatatataca acttagattt atcgcaaaga
     6961 agagcatata gcgttgctaa atttatagtg ggagatgaaa taggtgacta taaatacaaa
     7021 gataaagtga ctaagcatat tattgcaata ggaaggtcaa aagctgaact catcaaaaat
     7081 ggtgacaata gcgtgaatag agatgcatct agaagagtag aactaaaata cgaaataaat
     7141 attaatcaaa ataaataatt aaatttttat aaaaaaaaag aaggcagttg gtgtgagctt
     7201 taagccttct ttaaggaaaa agtttattta tttttgttga tacttaacat attatagttt
     7261 ttagaagcag tttgcaatat attaaacgta aatggtcgtt taaaatgcat aactggatac
     7321 tactgatgta aaatatttca ttttatataa tataaaaatt atacagtatc aattttctat
     7381 attattgtac tataatctat tatttaaaat attatgtaaa aaaatatcat aaaaacgcat
     7441 atcatgttat aattactaga gtattatgaa tatggaggcg tagtttgtga tagaaataag
     7501 tataaaaaat aaaaaaaatt ctaaaattaa aactttgata cttttattga tagtatctat
     7561 atcaatttat ttaactggtt gtactacatt agataatgga aataatcaat caaataataa
     7621 tgattctttg tctagtagta tatcaagtga taagaatgta aaaattcatt ttattgacac
     7681 tggaaatagt gatgcaatat tgattcaaga tggtaaaact tttatattga tagatggggg
     7741 agataatgat gatgagggat taatggtaga ttacctaaac aatcaaggtg taaaagatat
     7801 taaatatctt atagctaccc attctcatgc agaccattta ggtggacttg attctgttgt
     7861 caaaaacttt aatatagaaa atgtttttgt atcgaatggt tcagcagaaa caaagagtta
     7921 tagggacttt ataaatgctt tagcaaataa gggtttatcc ccaagtgtac cgcttgaaaa
     7981 taataagttc tatttagaag attcttattt tgaagttcta aatacaaatg ggggagatac
     8041 tactaatgaa caatctctag tccttgtata tactaatggg aatgataaag ttttatttac
     8101 aggtgatgct gaagaaggta ccgaaaaaga aatactccct aagcttgaga aagtagacct
     8161 tttaaaagta gctcatcatg gttctcgtag tagttcatct caagagtttt tagataaagt
     8221 aaatcctgaa tatgcggttc ttttagtagg caagggtaat tcatatggtc atccacatca
     8281 ggagacaatg aataaattaa aaaaaatggg agtaaaagta catagaagtg atgagtgttc
     8341 agatataata tttgaatcaa caggtgatgg agtatttaca agttgtaaag atggaagtta
     8401 taataaaggc gttagagaag atggaaactc tggaaaatca aacagtaata caagtaaaaa
     8461 tgattctttt aaaaataaac aaaatacatc caattcagaa gaaatagtgt attttactcc
     8521 aaaaggtaag agttatcact ctacaaaaaa ttgtagtggg ctttcaagaa gtaaaaaaat
     8581 attatcaggc acaattgcag aaagtaagaa aaatgaccca tgtgatatat gttatggaaa
     8641 ataacaaaaa aacaaccaat ttatctataa agcttctgaa ctttaaatca tttaaaaaat
     8701 aaaataaatg cataaaaaaa taagctataa tcaatagata acacatttta tctatacgaa
     8761 tagcttattt ttttaagtat tttttaatag ctatttaata actctaaata caccagttac
     8821 aatacctaca atttcaactt ctgagtcatt gagcataatt ggttccataa aatcattttc
     8881 tggctgaagt cttattaaat ttccttcttt aaaaaacctc ttaactgtag cagattcacc
     8941 atttataagt gcaactacta tctgagaatt taaggctgta ttttttttgt ctactacaac
     9001 gtaatcttca tctaaaattc ctgcattaat catactttcg ccttttactc ttaatacaaa
     9061 attgttactg ccctttacta aactggcagg gaatggaatg tattcctcta tgttttgttg
     9121 agctaaaatg ggttctccag ctgttatttg acctactaaa ggaagctcta ttatttcttg
     9181 attagctcca gaaacatcat ttactttact acgttctaaa acttcaatag ctcttggttt
     9241 agtagggtcc tttcttatgt atcccagttt ttcaagttta tttagatgag agtgcacagt
     9301 tgaagtagat cttaacccta cagcagtaca tatttctctt acagcaggtg gataaccttt
     9361 taatatgatt tgagacttta taaattccaa tatcaagact tgcttttcag ttagatctaa
     9421 atacataaca tcactcattt ccaaacatat tttgttttta tttccataca ctttatattt
     9481 ttaattataa cataatccat ttatatctgc aaacatttgt tcctgatttt taatcgtcct
     9541 caatccattc aaaattcttg actatatcac gttttacatt tttcttatga gctgcataaa
     9601 gctgagtagt tgtgacatta tcatggtcaa gtaagttttg aacatcatat atagaaaatc
     9661 ctgtttggat tagagaagtt gcagccgttg ctcttaattt atggggacta tatccattgt
     9721 ctctagtagt atcaagtgga atagatgtat atttttttac cagtgttctt atagcctttg
     9781 ctgtaattct cttattttga agagacaaaa ataaagcatc tttttcttca tcatttaata
     9841 gttcatctct tgttctttcg ttttgcaaat aatcttttat tacatgttca caagtatgat
     9901 ttataggcat taaaacttct ttcccacgtt ttctatatat tttaaattca cctcttgaaa
     9961 aattaaaaga ggagatattg agttctctaa gctcatttaa tctaagcccg tatgtaacaa
    10021 ataatgctag tatagctttg tcacgtaatt tagtttttct ccaatatacc ttttctttct
    10081 ctgtaagccc ttcacctgtt tctactgatt caagcatctt agcaacttca tctatctcaa
    10141 ggcgcttaat agcatctggc tgaggtttag gaagttttat tggattgaat ccatcagtta
    10201 tattattatc tatctgagag tttctgtata agaatttaaa tagagtagag atggatgatt
    10261 tttttcttgc taaagctcta ttgttatttt caaaaatcaa tgtgtttttt tctgtatttt
    10321 tgtaatatct actgcaataa tcacctaaga ataaatttat atctcttgat tttattgtat
    10381 taaaatcatc ttctgtaata tcttttacac aatctgcatt tgttaattct ttggtttcaa
    10441 tcaaatatga acaaaaaaaa ttaatatctt ctaaataagc aagtcgtgta gaaactgcaa
    10501 cagaaccttt taaatatata aaat
//
LOCUS       DBGJFU010000126        10516 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.127, whole genome shotgun sequence.
ACCESSION   DBGJFU010000126 DBGJFU010000000
VERSION     DBGJFU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.127"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            633..1283
                     /locus_tag="ACOIAS_003070"
     CDS             633..1283
                     /locus_tag="ACOIAS_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2702761.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1631..3676
                     /locus_tag="ACOIAS_003071"
     CDS             1631..3676
                     /locus_tag="ACOIAS_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HGZ2702762.1"
                     /translation="MKKITKERIENTMKKLFTSETFLEYQFISDCQISPDGGYTAFIV
                     KKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRNEKVKEAV
                     KNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRPIVEGLPE
                     EEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAITAPMFNV
                     VGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGFELYQNQV
                     ILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQTTKVD
                     GEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGNGLPEV
                     YTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVGKKYPA
                     VLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFGDIDYI
                     DFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIANWSGM
                     EGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDYRCYMQEA
                     FQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKDNIGQ"
     gene            3693..5648
                     /locus_tag="ACOIAS_003072"
     CDS             3693..5648
                     /locus_tag="ACOIAS_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HGZ2702763.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5664..6854
                     /locus_tag="ACOIAS_003073"
     CDS             5664..6854
                     /locus_tag="ACOIAS_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGZ2702764.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            8003..8377
                     /locus_tag="ACOIAS_003074"
     CDS             8003..8377
                     /locus_tag="ACOIAS_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2702765.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            8364..9236
                     /locus_tag="ACOIAS_003075"
     CDS             8364..9236
                     /locus_tag="ACOIAS_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702766.1"
                     /translation="MRRINMENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNG
                     AGKTTTIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMK
                     SIIAPSYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDE
                     PTSGLDPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILERE
                     KDTLLDEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERP
                     TIEDIMLAYIERRE"
     gene            9239..9877
                     /locus_tag="ACOIAS_003076"
     CDS             9239..9877
                     /locus_tag="ACOIAS_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HGZ2702767.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
BASE COUNT         3893 a         1210 c         1868 g         3545 t
ORIGIN      
        1 agtacactag catgatttgc agaacaatga attaatctat agtataatag tttaatttaa
       61 agtacaacga tttaatctat agtacactag catgatttga agtacaacga tttaatctat
      121 agtacactag catgatttga agtacaacga tttaatctat agtataatag tttaatttga
      181 agtacaacga tttaatctat agtacaataa cttgatttga agtataacga tttaatctat
      241 agtacaataa cttgatttgc aatacaatga attaatctat ggtataatag tttaatttga
      301 agtacaatga attaatctat agtataatag tttaatttga agtacaacga tttaatctat
      361 agtataatag tttaatttga agtacaatgg tttaatctat agtataatag tttaatttga
      421 agtacaacga tttaatctat agtacaataa cttgatttgc aatacaatga attaatctat
      481 agtataagag catgatttac agtacaataa tttaaattgt agtacaaaag tactatcaaa
      541 taagaatttc tactttaaaa atattactaa aagtaattaa agtccagtaa ttcaatataa
      601 tatggcaaaa gaatagaaat aaggtgagag tagtgaaagt aaagtcaaat ttagatgaaa
      661 ttttatatca taagattatt gaaagtctta ttcgtggtga atactctgta ggtcaaaaaa
      721 tattactaaa tgacctttgt gaaaaatttg aagtaagcag aacaccagtt gtacaagcag
      781 taaaaatgct aaataaagat ggagtgctta ctattatgac aaatggtaaa gtgtacgtac
      841 cagagtatga atatgatatg gtaaaacaag tttgtgaaac gagaacactg attgaaacat
      901 atgctttaga aaaaatgatg caagaagagg aagaggtttt tcaacaaaag ctagatgtaa
      961 taaaaagata ctctgataaa tgcgaggagt actatcgtca agaaaaatct gtagaacttg
     1021 cactagcaga tttggaatta cataaagcca ttgtagaagg ggcaaataat gagattttaa
     1081 atgatgtata tgttggtata caaggaagat ttatagtagt gaactattta attcgaccat
     1141 taaaaaatcg taattatgaa ggaacagtac aagaccattt tgaaatatta aaattcattg
     1201 aagacagaga tacaaaaaaa gcagtagata aactaagaaa tcatattcaa ggaactatta
     1261 aaagattctg tgaagatgaa taataagttt tgaagttact atatatactt atctattaag
     1321 ttgctaaatg tagtctaagt ataatctaat tatagtttaa acataatcta aatataatct
     1381 aataaatata atctaaatat agtgtaaata agtctaagta tgaattaaat atagattaag
     1441 tatagattaa gtatagatta agtatgaatt aagcatgaat taagcatgga ttaaatatac
     1501 tttaaattac aaatattaca aggaagccag aaggcttcct aaaaaaataa cctttataaa
     1561 attttttatt ttaaatttaa aattttttat tttaaattta aaataaaaaa tgaaagaggt
     1621 ggtagagagt ttgaaaaaaa ttactaaaga aaggatagaa aatactatga aaaagctatt
     1681 tacgagtgaa acatttttgg aatatcaatt tataagtgat tgccaaatat caccagatgg
     1741 aggatataca gcatttattg taaaaaaagc tgatatcaaa gagaatggtt acacaagcca
     1801 agtttatata ttaaacaata aaactggcga attaaaacaa ataacgtcta taaactctgt
     1861 tggagcatat gcctgggaag atgaaaatac tatcttgttc cctgctttgc gaaatgaaaa
     1921 agtaaaagaa gctgtcaaaa acggtaagca atgcatgagt tactatgctt tatctttaaa
     1981 tggtggagaa gcagaagaac tttttagact ccctatcaaa ggtggtaaat tgaatcctat
     2041 aggaaaaggt ttgtatgcag ttattgattc ttatgacaat gaccgaccta ttgtagaggg
     2101 attgccagaa gaagaacaac agaaaaaaat aaatgcttat aacaaaagac attatgaaca
     2161 tttcaaagaa attccttatg ctgtcaatgg ggagggatat attagcagaa aaagaaaacg
     2221 cctatatgta tacgacagta atataaatga gttgaaggca attacagcac ctatgtttaa
     2281 tgttgtaggt atgaaaataa gtgatggaaa aatcttatat atagggcaag agtttaaaga
     2341 tgtaaaaggt ttgaaaaatg gggtctatgt atttgataca aacacaaaca caaatgtctg
     2401 tatattagaa aaagataagt atattataaa aggttttgaa ttatatcaaa atcaagtgat
     2461 actaaaccta actgatgctt tatcttatgg aaatggagaa aatggagatt tttatactat
     2521 tgatattgat acgaaagaaa tgaaacttct ttcagggcat caacatcatt gtattggaaa
     2581 tacagtaaca tctgatgtta aaatgggtgc tggtcagaca acaaaggtag atggagaata
     2641 tatttattac acttctacag tagatatgga ctgcattatt gaaagaatac atattccaac
     2701 aggaaaacag gaaaaagtaa cacagacagg ttcagttgat tttatagatg taaaagatgg
     2761 caatattgta tgtgtgggtt gtttaggaaa tggattacca gaagtttata cagtagagaa
     2821 tggaacattg tgtaagaaaa cacatttaaa tgaccatatt ttagaggaat ataaaatttc
     2881 tgtgccagaa tacatagagt caaaaggaag ttctaaatgg gaaatacaag gatacgtata
     2941 taaaccagta gattatgagg tggggaaaaa atatccagct gtattggcga ttcatggagg
     3001 accaagattg acatatggac catattttat gcatgagata caggtattta caagtgcagg
     3061 ttactttgta ttcttctgta acccaagggg gtctgaaggt cgtggcaatg cttttgcaga
     3121 tattaggaaa cagtttggag atattgatta tatagatttt atggaattta cagatactgt
     3181 acttgaaaaa tatacagaca ttgataaaac aaaattggct gttgaaggtg gaagttatgg
     3241 tggatttatg acgaattgga taattggtca tacaaaccgt tttgctgttg cttgtgctca
     3301 gcgttctatt gcaaactgga gtggcatgga gggtacaaca gacattggtt attatttctg
     3361 caaaggacaa acaggtgctt ctcatatgga aaaccacgag cttcaatgga aacagtcacc
     3421 tcttgcttat gctgataaat gtgttacacc gactttattt ttacatggtg aaaaagacta
     3481 tagatgctat atgcaagaag cattccagat gttttcagca ttgaaaattc atgggtgtcc
     3541 tacaaaactt tgtctatttg aaggagaaaa ccatgaactg tctcgttctg gtagaccaaa
     3601 acaaaagcta caaagattag ttgaaatgct agattggttt tcagtatata taaaaaaaga
     3661 taacatagga cagtagaaag gagaatataa aaatggaaaa aataaagatt gaaaattcag
     3721 atatttttga atatatattc cctcatgata tttcttgttc acctgatgga aagcatattg
     3781 cttatattat atcaaatatc aacgaggaaa aagactgcta tgaacatgac ttatatgtga
     3841 tggacatcaa aacagaaaaa caaatacata tgactcaaac aaaagatgta acatcattta
     3901 gttggatttc taatacagaa ctccttttta catctaaaag aaataagcca aaatcaggta
     3961 caacagattt ttacactatt tctatagaag gaggagaagc aaaaaaagca ttttctattc
     4021 caaaagcttg tagcgtacct gtatcacttg gaaataaatt gtggttactg acaacaaaaa
     4081 atccaacgga ttctaaaaaa tcagaaccag atagggcagt agagggtgta gattattgga
     4141 catttacaga taaacctttt ataagagatg gagaaaattt ttctcaaaga agacgtgtaa
     4201 cattagaatt atatcaagaa ggtgaaaata taacaaaagc cattacacca aaattttgtg
     4261 aagtagcagg tgtagacgtt tcttctgata aaaacagaat tttatatact ggtcagattt
     4321 atgaagattg tgcaactcct ttttctggac tttgggagta tcatatagac agtggtgaaa
     4381 ccaaagaact tgtaccacaa ggaaagtacc aaataagttt ggcaaaatat attggcaaag
     4441 ataaagtaat gttgcaagct tcaacattag accgttctat cacacagaat catgatattt
     4501 ttatacttga tttgagtact aatgaaataa atatgattgc tagtccagat ggtatgtatg
     4561 ctacattgct ggatgtagat gcagtatatg gtggtggacg tagtaataaa gttataggag
     4621 acaaatttat tggtgcaaga atttgcagaa ctatgacaga atttaatgag tttgatacaa
     4681 agacaggtaa tatacgtatc atcacaaaag tagatgcttt tacatctttt gatatttacg
     4741 acaacaccat gtacacagta atgttgaaag attatgagtt agcagaaata tatagcattg
     4801 atatgactac aggtacaatg aaaaaaatga ctgctttttc taaaccatat ttagatactc
     4861 ataaggtttc attacctgaa aaattgacgt ttgtagcaaa aaataaagaa gaagttgatg
     4921 gttttgttat accacctata gatgctaaag aaggcgaaaa atatcctgct gtattgttca
     4981 ttcatggtgg gcctaaatgg gcatatggct atatgtttac acacttgaag cagtgtgtaa
     5041 catcaaaggg aatgtatgtg atttattgta accctcatgg tggtgacggt tatggtgaaa
     5101 aattcttaga aatggtagaa agatgggggt atgttgacta tgagcatctt atggaatttg
     5161 tagatacttg tattgaaaaa tatcctggga ttgatgcaga ccgcttaggg gttgcagggg
     5221 gaagttacgg tggttatatg acaaactgga taattggtca tacagaccgt ttcaaagcag
     5281 cagtttctca aagaggtatt agtaacttga ttacagcttc tttaattatt gactttgggg
     5341 atagaattat gaagcagact tgtggagata agacgccttg gaatcatgag gaggttcttt
     5401 ggaatcattc acctattaaa tatgtgaaaa atgttaagac accaacattg ttcttacata
     5461 gtgataggga ttatagatgt tttatggggg atactttcca aatgtttaca gcattaaaac
     5521 agcttggtgt agatacagaa atgtatttgt ttcatggaga tacacatgga ttgtctagaa
     5581 atggaagacc atctaataga attgcaagag caaatgctat tgtagattgg tttgaaagat
     5641 atttataata agggggttat tctatgagtg atatgttatt atggtttagt gtcgtattag
     5701 ttttatttgc tattggggat ttaattgcaa gcaaaacaaa agcaaaagta tcagctgtat
     5761 ttgttacact acttttattt ttgattctat ttgtcacaaa agcaatacca gctgatatta
     5821 ttgaaaaagc aggtatgacc gcagcagcta gttggagtgt tccaatgatt atgtttagta
     5881 tgggaacaat gttgaatgtt aaacaattta tagacgaatg gagaacagta ttgacagcat
     5941 ggcttggaat tgtagcagtt atagtctgtg tctcattatg tattcctttg tttggaaaat
     6001 caacagtatt gacaagcata ccagttataa atggagcttt acctgctact actattatga
     6061 cacaagcagc actagaaaaa ggactgacac ttgcagcagc tacagcaaca gttgtatttg
     6121 caattcagaa gtttattggt acaccaatag cttcaagagc agcgttacaa gaggcaaacc
     6181 gtcttttagt agaatatcat gaagcaaaat caaaaggcat tgatttagca aatgttgata
     6241 ctgataagaa agaagcagag aatacaggca caaaagtaaa acaagctttc tgtgaaaaat
     6301 acgataaata ttattctaca aatgtttgta tattttttat agcattattc tcttatctag
     6361 ggtatgaact ttcagagatt attcatgtaa attattctat tgtatgtctt gttgttggtg
     6421 ttattgtaac acgtattggt attgtgccaa aagacatttt ggaaaaagga aaaatcaagg
     6481 gatttatcaa catggttgtg tttgcagctg ttattccttc tttagcaaaa gtttctttga
     6541 cagatttaat tagtcttttt gtaccaattg tgggtatgtt tgcagcttct atcattggta
     6601 tattccttat gatgaaggtg ttaccaggtt ggaaaattat cggttctaaa cctcttgctt
     6661 ttggagtagg gttttgtcag atgttgggat tcccaactac atatttaatt tcaaatgaag
     6721 tgtgtaatgc tgttggagaa acggaagaag aaagagcata tttgatgtct aagattatgc
     6781 ctaagctggt agttggtggt atggcttgta tgatttctat tgtggtagct ggtcttatgg
     6841 taccaatgtt gtagataggt ttcatagtta aaaaagtggt attaatttat aggagaagct
     6901 aaaaagtagt attaatttat aaaataattt tatattagtt aatttgttat aagaatattt
     6961 tctttttata attataattt tgattttata agatagcaga ttttttattt gctatgctaa
     7021 tagttataat ttgtttttat ttgctaaaat gcaactttaa tttgatttat gtataatata
     7081 gggtatttaa taatattttt gaagaatttt tataaaaaat taaaaagagg agaaaaaagg
     7141 gagtgccaca aagtgaactt tttagttcta aggcactctt ttttgtacga aatcgaatat
     7201 attttcaacg ttttaaaagt gagaaagaga cttatctcta gtttgagata gccaatttta
     7261 attattaaaa ttattttttg ttttatttat caaagagtat tcgtgtttta gaagttagta
     7321 ttttttattt tatatataca aggttttccg tttctaatgt tttctttttt aaaataatta
     7381 gggtattcaa ttatagtaaa aggtggatgg tattttggag tgatattatt taaagcacag
     7441 tattgaatgt tgaaatcctc acattttaga gtattatctt ttgtaatcaa aaaaatcttc
     7501 tatacttata gcaaggttac ttctgcaaat attccataca taataatatc ccaatttaaa
     7561 tttttttaat atagagttta cctctagtct acactcttga ataactcttt cctctatcca
     7621 aattcttata aattcatccc atttattact atttatttat tattatctaa attttttagt
     7681 atgtttgtaa ttagaaatgg atttattcct ttatgttttt tataaatagt ttataactta
     7741 atttatcatt aaacaaatca ttcatacagg attacttttt cttttatata ttcaattctt
     7801 acttttaata aatatttaat acatcctatc ttatgtagtt ttaaaaagtg tttatggcta
     7861 ttaacaatct ctaatatttc attcatattt attttgtcat agtaatattt tacatctttt
     7921 atattgacaa aaaagtgctc taagtgtata gtgtgtatat gataacatca cactgaaagt
     7981 gataaaaagg gggtgataaa aaatgaaagt gctcatttct aacacatcag ataagccatt
     8041 ataccaacaa attaaagagc aaattaaaga agcaatattt actgaagaac tgaaagaagg
     8101 ggatgcaatg ccatccataa gaaattttgc taatgattta aaagttagta ttttgactat
     8161 tagacgagtg tatgaagaac ttgaaaatga aggctttctt acacgccaag ttggaatagg
     8221 aacatttatt tcaactggaa atttagaaat actaagagac tcaaagagac gtatagttga
     8281 acaaaaaatg gcagatatga ttaaagatgc aaaagcacta ggaattacac agatagagtt
     8341 acaagaaatg atggatattc tatatgagga ggattaatat ggaaaatatt ttagaagttt
     8401 ataacttaaa taagacttat accgattttt cattatctaa tataagcttt gcattaccag
     8461 aagattgtat aaccggattt ataggtgtta atggagcagg aaaaacaaca acaatccgtt
     8521 cgatattgga tgtcgtaaag cgtgatagtg gaaaaataaa aatctttggt aaagatttcg
     8581 atggaaatga gaaggaatta aaaaatcgaa ttggagttgt ctttgatgat tcatatttct
     8641 atgaggagtt gtcaatggca gaaatgaaaa gtattattgc accttcatat gataattggt
     8701 gtgaagaaga ttatcaacaa aatatgaagc gttttaatct taacccaaaa caaaagattg
     8761 atacactttc aaaaggaatg aaaatgaaat ttgctttagc attagcatta tcgcataaag
     8821 ctgagttttt aattatggat gagcctacaa gtggtttaga cccactaatc cgtagtcaga
     8881 tacttgacat actaacagga tatatgaaga aatgtggtcg tggtgttttc ttttccactc
     8941 atataacatc tgatttagac aaaatagcag atatgattgt tatgattgac aatggacgta
     9001 ttatattaga gcgtgaaaaa gatactctat tggacgaaca tagaaaagta aaaggggata
     9061 ttcaatttct aaatgatgaa accaaaaaat tatttttaaa tttagaaatt acaggttatg
     9121 gttttatagg gattacctca aaaattaatg aagtgcaaaa aaatatgcca gatattttaa
     9181 tagagcgtcc tacaattgaa gatattatgc ttgcatatat tgaaaggaga gagtaattat
     9241 gttgattcat ttagttaaaa aagatattct gattgtaaaa aaatatgtat tgttgatatt
     9301 atttattgca ataggaatcc cattatttat attatggcgt ttacctccat ttgcacaaat
     9361 attgggattt gtactatcaa ttatattttc agaatttatg ctgtgtcagt atctattatt
     9421 aaaggagagt caatattcaa aagcatctgt attactttgt tcaactcctt atcctagacg
     9481 tggcttagtt caatctaagt atgttttatt tgcaatggta tttatctatt gtacattagt
     9541 ttactggatt gaaaatttac ttattccaca aataggaact tttgacttga catatatttt
     9601 gattgtttta ttaataagtt ctattattta tggagtttat atgcctattc aatacaaatt
     9661 aggatatgaa acaacaaaat tctttttcgt gatgataatc atggcttcat catttgtatt
     9721 accattaact atagctcaca gcaattttga aattccattt atatatcagt atccatccat
     9781 gatagtgaat attgtattgt tccttttaag tttaattata ctgttagctt caatgtttat
     9841 ttctattaaa atttttagta ataaggagct tgtataaggt aagggggact tttatatgcc
     9901 acatttacca gattgggctg ttttactttg tatcttactt ccaatagtat ataaagcttg
     9961 tcagatattt aaaagtaaaa aatagagtat atatttatat tgccattttt attgagtgaa
    10021 gttagatggt atgaaaaggt tacttgaaaa taaagttact tggtgtggga gagtaacatc
    10081 ataatattat agattatatg tttcttgtga atcaatttgt attttttaat agggaatatc
    10141 cagtaatttt gttttccatt gattattttt caattatgag tatgattgtg tgtattgtat
    10201 accatctttt aaggtgtttt taaaaaatta tatcgtcaaa atataaatct aaggaatcct
    10261 ataaataaag ctttttaatt ttagaaaact aataaaaagt aggacgttaa taaaaagaag
    10321 ctgtctcaaa atagagataa gcttcttttt cattgctgaa atgacgaaaa tataattgat
    10381 ttaatataaa aaagagtgct ttatgaacta tttattttga acagtagata cacttgcact
    10441 caatttagtt actcctaagc tttatgaact aaaagtttat tttgaagctt atttttatta
    10501 aaagagattt tatttt
//
LOCUS       DBGJFU010000127        10505 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.128, whole genome shotgun sequence.
ACCESSION   DBGJFU010000127 DBGJFU010000000
VERSION     DBGJFU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.128"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            191..3088
                     /locus_tag="ACOIAS_003077"
     CDS             191..3088
                     /locus_tag="ACOIAS_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2702768.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLNSGYEYSVLYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKYKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            3409..3621
                     /locus_tag="ACOIAS_003078"
     CDS             3409..3621
                     /locus_tag="ACOIAS_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2702769.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            3634..3855
                     /locus_tag="ACOIAS_003079"
     CDS             3634..3855
                     /locus_tag="ACOIAS_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HGZ2702770.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            3955..6111
                     /gene="feoB"
                     /locus_tag="ACOIAS_003080"
     CDS             3955..6111
                     /gene="feoB"
                     /locus_tag="ACOIAS_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HGZ2702771.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6137..6289
                     /locus_tag="ACOIAS_003081"
     CDS             6137..6289
                     /locus_tag="ACOIAS_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HGZ2702772.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            6469..7179
                     /locus_tag="ACOIAS_003082"
     CDS             6469..7179
                     /locus_tag="ACOIAS_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HGZ2702773.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            7264..8034
                     /locus_tag="ACOIAS_003083"
     CDS             7264..8034
                     /locus_tag="ACOIAS_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702774.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            8027..8758
                     /locus_tag="ACOIAS_003084"
     CDS             8027..8758
                     /locus_tag="ACOIAS_003084"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702775.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            8792..9751
                     /locus_tag="ACOIAS_003085"
     CDS             8792..9751
                     /locus_tag="ACOIAS_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2702776.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(9849..10445)
                     /locus_tag="ACOIAS_003086"
     CDS             complement(9849..10445)
                     /locus_tag="ACOIAS_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HGZ2702777.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4056 a         1090 c         1889 g         3470 t
ORIGIN      
        1 tgcttttatg acttataaga aactaatatt agatagtgta tttaaataga taataataaa
       61 ttcattatag ttgaataaga atattatatg agaatattgc aatttgatat atttaccatt
      121 acaataaaag tagttacaac tagaaaacca aataagttaa agtaagttgg caaattggag
      181 gtttttaaat atgaaagcat ttaaaaagca cttcttgata ctttctttga tatttttact
      241 tattatatca tgttttaaca atatactatg ctttgctgat gagtctgaaa attcaaaaaa
      301 aataagagtt ggatattgtg atgactacgg aataatatcc tcatcaaaag agcattctta
      361 tacaggatat ggttatgatt atctaagaga aatctcaaaa tatacaagat gggaatatga
      421 atttgtaaaa gggtcttggg aagaatgttt agacagacta gaaaaaggtg aaatagactt
      481 acttggacct ttacagaaga atgatgaaag aaataaacta tttaattttc ctaaactaaa
      541 ttcaggatat gaatatagtg tactatatac aaaagatagt aacaataata tgttttatga
      601 agatataagt tcctttaagg gaatgagagt agctgtactt agaggaaact ttcataatat
      661 tgcatttgag aagtatagag aagaaaataa tttttcagtt gaatatatct actgtaattc
      721 tattgatgaa ttaattgagt ctgtcaatga aaaaaaggca gatgcatttg tctgtggaag
      781 tattataaat gctaaaggaa tgaaaatagt tagcaaattt tcagtagagc ctttttattt
      841 tgctactgca aaatataaac ctaatttggt aaaagaatta gattatgctt taaaagaatt
      901 aaaaataaat gatatgtatt atgagttgga attatataaa aaatatttca aaagagaggt
      961 caataattcc atagcattta ctcggcaaga aatgaatttt attaaagcaa acccaaaact
     1021 tactatggta tatgattcag agtggtctcc tgtagagtat tatgataagg aaagcaattc
     1081 gtttaaaggt ataagttcca atttaatgtc aattatatca aaaaaatgtg gtataaaatt
     1141 tgaatatatt aaaacaaaaa attataatga gagtcttgat tacataaaaa gtggtaaggc
     1201 aaatatgcta tgtggctcaa tcaatgaaaa tgataaggca aaacgattta atatgaaatt
     1261 gacaaatcca tacataaata ttccaatgat tatggtagga aaactggata ccaatctcaa
     1321 taatgattta aatattgctt taacgagtag ttataagtct atcgattctt atatagaaaa
     1381 aagttttgaa aatgctaaga caacatctta taagagtata gaaagttgtc taaacgctat
     1441 aaatgaaggt aaagcaaact taatgatact tagttcttat cagtttgatg aacttttaag
     1501 agaaggaaag agtgagaatt tatctgttat atctgtttta gatacaagct atgggatgcg
     1561 tataggggta tcaaataaaa ctgaccctat attagtatct attttaaata aatcaataga
     1621 caaaattact gaagaagaat taagtgattg tatatattct aatactattg acaaacctta
     1681 taaagtacca tttggtgtga tttttaaaga atattcaata caaattattt cttttgtatg
     1741 tattttattc ttaattgcta ttaaatatat aatttataat aaaaagaaga aagaggatta
     1801 tctaaagaag atagcatata cagacccatt gacaggagca gatagtatag ataaatttaa
     1861 aataaattcg aataaactgt ttgctaaaaa taatccagaa gaatatgcat tgttttatat
     1921 agatgttgac aaatttaaat atataaatga tatgtttgga tatgatatgg gaaatgatac
     1981 tttgatacat atatcaaata ctattgcaag tgaattaaag gaagatgaaa tttttgcaag
     2041 agtttctgct gaccattttg ttcttcttat taagtataag acagatgatg atattaaaac
     2101 aaggttaaac aatatttaca ataaggttca aatacttagt aatcctaaaa taaactatta
     2161 taaattgatt ttagattgtg gtatatataa aataagtaaa agtgataatg acataaatac
     2221 tattatggat agagctaata ctgcaagaaa aactataaaa ggtggtcata aaaattcatt
     2281 tgctttttac gataaggaaa tgcataaaaa aatattaaaa gagaaagaaa ttgaaaattc
     2341 aatggtagat gctcttaata atggagaatt tattgtttat tttcaaccta aatatagtct
     2401 ttcagattat caaattatag gggcagaggc tcttgttcga tgggataatc cacaaaaagg
     2461 tctaatacca ccaatagaat ttattcctgt atttgagagg aatggtttta ttgtaaatat
     2521 tgatttctat gtatttgagg aagtgtgtaa aaagattaga gaatggatgg atgaaggaca
     2581 aaaagtagta cctatatctg taaacttgtc tcgaatgcat tttgtaaata gtaattttat
     2641 agagaagttt aaattaattg tagataaata caaaattccg acaagactta tagaattaga
     2701 gctaacagaa acagcagtat tagataatat agaagggtta cttgatacaa tgaacaattt
     2761 gaaagaaaag ggatttgtaa tctctatgga tgattttggt acgggatatt cctctcttaa
     2821 tcttttaaaa gaattgcctg ttgacatatt aaaactagat agagcatttt ttacagaaaa
     2881 agatgagagt aataatgaaa aaattgttat atctaatgtc attaaaatgg caaaggaatt
     2941 gaaaatgaaa gtaatatcag aaggtgtaga gactatttcg caagtagaat ttctaaagca
     3001 aattgggtgt gacatggtac aaggatattt gttttctaag ccaatgcctg taaaagagtt
     3061 tgaaaaaatt gcttttaaaa aagaataaag caatgtgtaa gtaatgatat atataataaa
     3121 ggtgctttaa tattagttga gtatactatt ttagagcatc tttatttttg tattaatttt
     3181 tacgatttta ttaataaaat aaatcaaatg agaaaaaaaa tcaaaagaat acaataatca
     3241 cttgcaaaaa atatactatt gtgatagact taaatcaaaa ttaggaattg taaaaagaag
     3301 gaaaaaatag gatattattt taatagatta agatttaagg tattgtaatt tatatataag
     3361 ataacgattg cttgtagatt taaattagtt taagaggagg gatattatat gatgccattg
     3421 atactatcaa atacaggtga agaagtagtt ataaagaaaa tttcaggaag tgataagacg
     3481 aggacttttt taaatagtat aggttttgta gtaggtacaa atgttaaaat agtttctaag
     3541 atagatggta acttaataat agacgtaaaa gatactagag ttgcattaga taaaaaaatg
     3601 gcaagtagaa taataatata gggaggtaat gacatgaaca gactgaagga tattaagtgt
     3661 ggagagactg taaaagtcaa aaagttagaa ggagaaggag ctacacgaag acgtataatg
     3721 gatatgggga ttacaagagg cgttaatata tttataagaa aagtagctcc tcttggtgac
     3781 ccaattgagg taacagtcag agattatgaa ctatcaattc gtaagtcaga tgcagaaaaa
     3841 attattgtgg aataataaag attaattaga gtagagttaa tgtaattata taatgagaat
     3901 gattttctat atcatgaata gtgatgtatt taaataataa tggagggaat ggaaatgtca
     3961 ataaaaatag gtctcatagg aaatcctaac tgtggtaaga caacaatgtt caatgggctt
     4021 acaggctcat cacaatatgt tggtaactgg ccaggagtaa cagttgaaaa aaaaggtgga
     4081 aaattaaaag gaaataaaga tgtggaaata gtagatttac caggtattta ttctttatct
     4141 ccatatacat tagaagaagt agttacacgt aattttatgc tagatgataa gccggatgca
     4201 gtaataaata ttgtagatgc atcaaatata gagagaaatt tatatcttac aactcaagtt
     4261 ttagagctag gtataccgac cgttatagct cttaatatga tggatattgt aaataagaat
     4321 ggtgataaga taaatattaa agaactatct gaagttatag gttgcccagt ggtagaagta
     4381 acagcagtta aaggtcaagg aattatggag gctgctgaaa aggcagttga acttgcaagt
     4441 agtaacaata aattaaattt caaattaccc tttgttgatg aaagtaagga tgcaatagaa
     4501 aaaattgaaa aaataataga agaaaagact ccatatatag atgtagaaac acgctggtta
     4561 gctataaagc tatttgagag agatgaaaat gttatacaaa aacttgatat ttctaaaaca
     4621 atattgaata gtattgaaga aataactaga aattgtgaag atgaattgga tgatgatagt
     4681 gaaagtataa taacagccaa tcgttatgaa tttattagtt caataatttc aagtataata
     4741 aagaagaatc gaaaaggtaa ggaaactgta tcagataaga tagataagat tgtaacaaat
     4801 cgtatactag cattacctat ttttgcactt ataatgtggg gagtttacta tatagcagta
     4861 agttcattgg gtactatagc aactgattgg acaaatgatg tactgtttgg agaaattatt
     4921 caaggcaatg taagtaattt tctagcttct ttaaatgtag cagagtggtt acaaggactt
     4981 gtagttgatg gtctaattgg tggagttgga gcagtactag gatttgtacc acaaatcatg
     5041 cttttattct tattattatc aatattagaa gattgtgggt acatgtcacg tgtagctttt
     5101 ataatggata gaattttccg taagtttggt ctttcaggaa aatcatttat accaatgctt
     5161 ataagttcag gttgtggagt tccaggagtt atgtcaactc gtactataga gaatgataga
     5221 gatagaaaaa tgactattat gttaactaca ttcattccat gtggagctaa gattccaata
     5281 atagcattat ttgcaggggc attatttgga ggtgcttcat gggtagctcc atcaatgtat
     5341 ttcttaggta ttgcaatgat aatcatttgt ggaattatat taaagaaaac tagtttattt
     5401 gcaggagaac catctccatt tgttatggaa ttacctcaat atcatattcc aagtgcaaaa
     5461 ggtgttttaa tacatatgtg ggatagagga aaagcattta taatcaaagc aggaacaatt
     5521 atatttgttg cttgtggagt aatctggttc ttacaatcat ttaattggtc attacaaatg
     5581 gtagatgctg gagatagtat attagctagc ttaggaaata tagttgcacc aatatttgct
     5641 cctcttggat ttggaaattg gcaatcatca gtagcaacag ttacaggtct agtagctaag
     5701 gaaaatgtag ttggaacatt tggagtttta tttggtatat cagatgcaac agagcaagac
     5761 ccaacacttc ttgcttcagt tgcaagcatg tttacagtag ctagtgcgtt tgcatttatg
     5821 gcatttaata tgctttgtgc accttgtttt gcagctattg gagccataaa aagagaaatg
     5881 ggttcatgga aatggacatg gattacatta ggattccaaa ctttaacagc gtatataatt
     5941 gctcttctta taaatcaagt tggaagttta gttttaggta ctggaggaag tattgctggg
     6001 gcaataatat ctatatttat agcagtagca gtagtctttg tagttttaac ttattcaaat
     6061 aaaaatatga aaaaagaaaa aatgggtaaa ttaagttata tgaaaaatta attcatataa
     6121 ataaggagtg atgaatatgg ctacatttat tatagcagct attgtagtag tattaatggc
     6181 actagctgta atgtatatgg ttaaaaattc aaaaaagaat ggtagcagtt gtggatgtgg
     6241 ttgttctgga tgctcaagta gtaagagttg tcatagcgct aagagataaa taggataaga
     6301 gcctgataaa aagttaaatt gctttattat caggttctta ttttttgtat aaataacaaa
     6361 tttcatattt gaagtaggtt agactatgct gtaattttac tgtataatta tatataaata
     6421 tatgattagg agggattatt gctaaattaa gcaatataag tgaatattat gtttaaattt
     6481 gatgttacaa acgagcttat gtgtatagca aggggaagtg gtaaattttt tgcaaaaaaa
     6541 ggtgctatgg tagcctttaa aggaaatttt aattttgaaa aattgctgtt aggtccaagt
     6601 aatggcggtg gattgggaag agcattatta ggtcatgttc agaggtcatt gacaggagaa
     6661 caaatgccaa taatggtggt tgaaggtgaa ggtgaaattt acctagctca aaatgcttac
     6721 catgtagatg taatatcaat tgacccaggt gattccattt atgttgagag tgaaaactta
     6781 ttggcatttt ctgaacaatt aaattacagt gtaaaattga ttggttcagg ggtaatatct
     6841 caaaaaggtc tttttactac acatttagta aataaaacag gtcaaaatca agatgttgct
     6901 ataataacag atggtaatcc tctaattctt gaaggacctt gttgtgttga cccagatgca
     6961 ttagtagctt ggactggaag agaaccatat cctaaagtat caaaactatc atggaagact
     7021 tttattggtc agacttcagg agaatcttat catatggaat ttgttgaacc tggacaaata
     7081 gtcattatac aaccatctga aagattgtct ggcttaaaaa cagattctac accttctgat
     7141 agaatgagtt caggtggatt aagacttggc atagattaaa tcttatgata tagcttaatt
     7201 attatttaag atattaaaaa ataaaaaggg tagcagggat agatatgtta aaaggagttt
     7261 tatatgtttg agaaaattca taaaaggctt aaaggatatt atattttttt aataatttta
     7321 attttatgga agatagtatg tgttttgggt atatggagta gctatattct gcctcctcct
     7381 gaaatagtct tagatacttt tataaaaatg atatatgacg gttctatttt cttaaatgtt
     7441 tatgccagtg taagaaggat attaataggt tttttaataa gttcattgat ggctatacca
     7501 cttggagttt tatttggagt aaataaaaaa atgtatgaat atttcaaacc actatttgaa
     7561 tttatgcgaa gtacacctcc actagctttg gtgccaatgc ttattttatg gtttggtata
     7621 ggtgaagaat ctaaaattat aattattatt ttggcatcct tttttccaat atttttaaat
     7681 acattgaaag gtataaaaaa ttgtgacaat aaattaatag aagtaggtaa atcttttaat
     7741 ttatctaaaa agagaatttt ttataaaata ataatcccta attctatgct ggatattgtt
     7801 gttggactta gtttagggct tgggtatagt tttagggcta taattggagc agagcttatt
     7861 gcagcttctt caggactagg atatttgatt tctgatggaa aagatatgtc tagaactgat
     7921 gttgtattgg taggaatttt tgttattgga tttttaggta tactttcaga ttatctattt
     7981 tcaaagataa taacaaaatt tagcaaaggg aagcaggttg atttaaatgg ttagaagtgg
     8041 atttttgcta gagaatatat ataagaaata tttagttgat aataaagagc atttagttct
     8101 tgataatata tctttaaata tatctagtga agagataact gttatattag gtgaaagtgg
     8161 atgtgggaaa acaacgctac ttagaatttt agcagggctt gaaaatgcta ctagtggaaa
     8221 catatatttt tttaataatg ataaaaagtg tactccaaaa gtaggaatgg tatttcaaga
     8281 aagtagactt atgccatggc tgaatgtaag tgaaaatata cttttacata ctgaaaaaga
     8341 taatagaaat aaagttgatt tagacaagta tttaaaaatg atgaagcttg aaaagtttaa
     8401 aaactcatat ccaaatgaat tatcaggagg aatggctcac agagtaagta tagcaagagc
     8461 actttccttc aatcccgata ttttgctgat ggacgaacca tttgcagcac tagattactt
     8521 cacaagaaga aaaatgcaaa aagaagttgt aaatatccat aaaaatacta aaaaaggtgt
     8581 agtatttgta actcacaata ttgaagaagc tatggaaata gcaaagaaaa taattgtttt
     8641 tagtaaaaat aaaaggatta aacaatttag tgtcgaagat gaatataata gagatttaac
     8701 taaaaactat tatataaact tgaaaaaaga aatattaagg gaattggggg agttttaatt
     8761 gattaaaaga aacaaaaaga ttttagcagt aatgacatct ttaataatgg tgttggttgg
     8821 gactgtagga tgttcaacaa caaatgataa gaaagatgaa cctgcaaaaa aagaggctag
     8881 tttacctaag gaaataaatt taacttatgt aaaatcacca cttaatgttc ctagtatatt
     8941 agaaaaaaag gatgatttat ttggaaagga atttaaaaag gacaatataa aagtcaattt
     9001 ccatgaattg acaacaggac cagagcaaac tcaagcatta gcagcaggtg agctagactt
     9061 tttacatgca ttaggaggaa cgtctgcaat aatagctgca tcaaatggtg ttggtcttaa
     9121 aattacgaat atttatagcc gttcaccaaa aggatatatg cttataacta ataatgataa
     9181 tattaaatct ataaaagact taaaaggtaa aaaaattgta ggtccaaaag ggactatact
     9241 tcatcaactg ttgattgctg cacttgctaa agaaggctat actatagatg atgttgaata
     9301 tgtaaatatg gatattccta gtgctgcttc tgcattagaa agtaaaagtg ctgatgtagc
     9361 aatgcttgca ggtccagtgg cattgaaaac gataaattca ggtgccaaaa tgattgtaaa
     9421 tggagaagga ttagtaagtg gaataatagt tactgctgta agtgatgact ttgctgaaaa
     9481 atatcctgaa ttagtaaaaa gatttatgaa ggttcatgaa gaaactttaa aatatatgaa
     9541 tgaaaacaag gatgaagtga tggatgttgt atctaaagaa gttggtttat ctttagatga
     9601 aacaaaagaa atgtattctt ggtatgactt tagttctaaa ataacggaca aggatataaa
     9661 agaattagaa gatacacagg aatttttaat gtctaatgga atgcaacaaa agaaaataaa
     9721 tattaaagat atgttgtaca atcaaaatta gaatacttta aaatattaaa gtggaaaata
     9781 cacccttatt tattatggaa actatatggt aaaagagggt gtatttttat tattatacaa
     9841 aaatacaatt aaaaacagct tactatttta tctacactta tgtagtctag taattttctc
     9901 attttcttac ggtgaagtaa aaacttcgaa tatgtgtcct cggattgttt tgtgtcacca
     9961 taaactttcc aatatttaat catagaaact tcataattac tatatcccat aaatccaatc
    10021 acatctttaa gaggatatcc taaaaaaata cctatttcat caggaaaaac atcactctta
    10081 agtttatcta ctaaatgctc taaataagca ttaacattcg tattttgttt gtatcctaaa
    10141 aactttaaaa agtttactgt ttttttacat tttaattttt ctgataatgc ttttttattt
    10201 acaaataaga tttttaatcc ctttttttct ttatcaataa ctataaaatc tattttctta
    10261 catttatata gatatttata aatatcatta attttttcct ctctatttac atcagtaaac
    10321 gaaatattta ttatttcagc aggtttagag cctaaaaaga ctggacctaa aacttctaaa
    10381 agccatttta tatatgatga ttcaatttta tttttacaac aaaattgtga attacaacaa
    10441 gacataaaag ataacctcct tcaaatgaaa attaatatca agtaattaga ttatactatg
    10501 aatga
//
LOCUS       DBGJFU010000128        10484 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.129, whole genome shotgun sequence.
ACCESSION   DBGJFU010000128 DBGJFU010000000
VERSION     DBGJFU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.129"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            64..372
                     /locus_tag="ACOIAS_003087"
     CDS             64..372
                     /locus_tag="ACOIAS_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702778.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(534..2324)
                     /locus_tag="ACOIAS_003088"
     CDS             complement(534..2324)
                     /locus_tag="ACOIAS_003088"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702779.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(2324..4051)
                     /locus_tag="ACOIAS_003089"
     CDS             complement(2324..4051)
                     /locus_tag="ACOIAS_003089"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702780.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(4296..4562)
                     /locus_tag="ACOIAS_003090"
     CDS             complement(4296..4562)
                     /locus_tag="ACOIAS_003090"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HGZ2702781.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            4860..5972
                     /locus_tag="ACOIAS_003091"
     CDS             4860..5972
                     /locus_tag="ACOIAS_003091"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGZ2702782.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(6336..6845)
                     /locus_tag="ACOIAS_003092"
     CDS             complement(6336..6845)
                     /locus_tag="ACOIAS_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGZ2702783.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVLVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(6847..7494)
                     /locus_tag="ACOIAS_003093"
     CDS             complement(6847..7494)
                     /locus_tag="ACOIAS_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGZ2702784.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(7508..8680)
                     /locus_tag="ACOIAS_003094"
     CDS             complement(7508..8680)
                     /locus_tag="ACOIAS_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2702785.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            8948..10324
                     /locus_tag="ACOIAS_003095"
     CDS             8948..10324
                     /locus_tag="ACOIAS_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702786.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILSNYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
BASE COUNT         3661 a         1723 c         1270 g         3830 t
ORIGIN      
        1 tgtaaaattt tttagaagct tctaatgtta tgctataaaa ctatcttgag aagggctgat
       61 tgaatgttta ataaaatata tgaaaagaaa gaatttatag tttttcaagt taaaaaaggt
      121 tatgtagttt ataatactag aaagagtttt gaagaaggtc atacacattt aaagcatttt
      181 gaagcagcaa agacagctat tgatttagca ataaataaaa agattcctag aagcaaagat
      241 ggatattatc tgactagttt aattcgcata agtgatgatg gttattacat tgataagtta
      301 agcgaattgt tatatgttag agaacaaaaa ggtaaaaaag aaaagtactg taattctggt
      361 tatcagatgt aacaagacta gttgtgaatt ataagtacca atcataagta cctttggtct
      421 taaaatcaaa gagtatcagt aaatctataa aaaaacaact tactgatact ctttttaatt
      481 atatgattaa tatttgaatt tgatacaaaa ttttatacaa aagtttatgt tatctattct
      541 gattctttgt ctgcaaattg gctattataa agcttctcat aaaatccacc cttttccatt
      601 aattcatcat gattcccctg ctctataata cttccattat tcataactaa aatcaagtca
      661 gcattgcgaa ttgtagaaag tctatgtgct ataacaaaac tagttctacc attcataaga
      721 tttctcattg ctttttgaag cataagttcc agcctagtat caacagaact cgtagcttca
      781 tcaagtatca atattgaagg gtctgatata aaagctctag ctatggttaa aagctgtttt
      841 tcccctagag atatattaga tgcttcttca tttaaaaaca tattgtatcc atctggaaga
      901 gtggttataa agtgatgaac atttgctact tttgctgctt gtataatttc ttctttagta
      961 gctccaaatc taccatattc tatattttca aaaatggttc cattaaataa ccatgtatct
     1021 tgaagcacca taccaaacat tgaacgcaaa tctttacgtt tcatatctct tatatctact
     1081 ccatcaattt taatagcacc tttctttaca tcataaaacc tcatcaataa gtttattaat
     1141 gttgttttac ctgctccagt aggtccaaca atagctacca tctgaccact ttttacttct
     1201 gcatttaaat cttctattaa agttgaattt tctccatatc caaaattaac atgctcaaat
     1261 gtaacatttc cttttacatt ttctatctta actggatttt caacatcagg tatttcctct
     1321 tcttcgtcta aaatttcaaa tacacgttct atagctgcaa aagaagattg tattactgta
     1381 gataactgag tcatttgtga aagtggctga tttatctgcc atatataacg cacaaatgct
     1441 tgtaaatttc ctactgttat tcctccactt actgcatata tagaaccgac aactgccaca
     1501 gctgcaattc caaagtaagt tactaatgaa accataggat tcatcatact tgatataaat
     1561 tgtgccttaa atccattttc acaaagttct tgatttactt ttttaaattc ttttatagaa
     1621 tcttcttctt taccatatag ttttatttca ttaaatccag tatacatttc ttgtactttc
     1681 ccatttaatt ttccaagagc attttgttgc aatgaaaatc tactttgaga tttttttact
     1741 acaaatttag atacaaaata actaattgga attataaaaa gagctataag agccatatat
     1801 atattaattg taaacatcat tgaaagtgcc aaaattatga ctaagattga gtttacaatt
     1861 tgcataaaac tctgctgcaa tgcatttgat attgtatcta catcatttga tatacgactt
     1921 aatacatctc caaaactatt actatcaaaa taactaatag gcaatcttct tattttattt
     1981 tcgacttcat ttcttaaatc tctcattgtg ttttgaatag cattggtaag tagaaaactt
     2041 gaagtatacg taaaaattgc attggcaaaa tatatacatg ctaaaatcac caatattttt
     2101 gttacataat caaaatttac ggaagcccct ggtactcctt ttgcgatact aattacatct
     2161 ttagtcaatt gtgttattat caatccttca gttttaggtg caagtgcatt catagtagct
     2221 gctgaaatta tacatataat agctataata aaggaaaatt tatatttctt tacaaatgga
     2281 agaagtctac caattgtttt cttaaaacct ttagactttt tcattattct aattcctcct
     2341 tttttaactg agatgttgca atttcatagt atatttcaca gttttttaat aattctttat
     2401 gagttcccat tcctacaact tgtccttcat tgagtactat aatcttatca gcatcaataa
     2461 tagaactaat tctctgggca acaatcaata caattgcatc ttttgtttcc ttcttcaatt
     2521 tagcacgaag tattgcatca gttttaaagt caagtgcaga aaaactatca tcaaaaatgt
     2581 atatttcagg tcttcttata atagcccttg caatagaaag tctttgtttt tgacctcctg
     2641 atacatttgc tccaccttca ctaatcaatt catcaaactt tctaggtttt tttgatataa
     2701 actcatatgc ttgtgcaact tttgcggaat attctaattc tgactctcct gccttatgtt
     2761 tcccaaatct aatattattt gctatgctac cagtaaaaag cagtgatttt tgaggtatga
     2821 atcctatctt ttttcttaaa gcttttaaat catattctct tatatctact ccatttatct
     2881 taatactacc ttctgttaca tcataaaatc taggtatcaa gttaattaga gtacttttcc
     2941 cactaccagt acttcctata aaagcaactg tttcacctgt ttttgcagta aatgaaatat
     3001 ccttaagtac agaagcttct ccatctggat aggtaaaagt tacattatca aattctatga
     3061 tacccttatt ttcagtatcc ttaattccat tttcaggatt ttttatcaat ggttcttcat
     3121 ttaaaagttc ttttattcta tttgcagata cctctgctct tggatacata ataaatacca
     3181 ttgaaaaaag cataattgaa aacatggcat ggaaaagata ttccaaaaat gcaaccaact
     3241 gtccaacttg aagctctcca atatttatct tctcacttga aatccaaaat acagccaaaa
     3301 cagctatatt taatagtaaa aagaatgctg gttgagtaat tgacataagc ttaaatagtt
     3361 tcttagaaac attagcataa tatgtatttg ttttttcaaa tcgctctgtt tcataatcat
     3421 catttccaaa agctctaatt actcttattc cagttaaatt ttctctcgat ataaggttta
     3481 atttatctag tcctttttgt tgttttgaag atattgcctg agatactttt gctattattg
     3541 ctactccaat aattataaaa ggtactgata tagccaatac tatagaaagt tccacacttg
     3601 ttctaattgt cattattata cttataatga acataactgg agttaataag gcagtcctta
     3661 gtagtatatt aacaaattgt tgaatttgaa atgcatcatt cgttgttctt gtaatcattg
     3721 aagaaattcc aaatttattg tattcagtat gtgaaaattc ttgtgatttc tcaaatatgt
     3781 catttcttat atctctggtt acacttgttg ataccttagc agaacaataa cccaacaata
     3841 tactacctac aacccctata atagaaataa taacaatcac tattcccatt tgttttatgt
     3901 aagctatatt ctgatttgca ataccattgt caattatttt agctattata gttggaatcc
     3961 ccaactcgac taatgcaaaa ctaaatatag ccagtatgtt taatactatt aataacttat
     4021 aattctttaa ataacttaaa attaatttca tatttttctc ctttcttaat ttttgagata
     4081 gtgtaatcat gtaataatac taaatttata aatgcttaat tttataaaaa aaggagaaaa
     4141 gagttaacgc actctcttct ccagcatatt ctttttaata caccctccga caagtcgctt
     4201 atatgttaac atatttgaca agtaatgtca attcatatag ccaaaagtct tttaaatgct
     4261 tcatttcata acattgtaac aaattggcat gtaatttatt tttgtttttt actgtattcc
     4321 aattttacaa gttctccaat attactagct ttcttaaaat tcaaattagt cttatctata
     4381 ccagacttaa atagtgaaat ccctctcccc aatattattg gcaatatata tatataataa
     4441 tcatcaatta agttatcttt cataaataat tttactatat cgcctccacc aactatccaa
     4501 atatcttttt tatccttctc ttttccaatt tgctcaatta attttttagg ttctaaatta
     4561 atttatagtg tgattattct atgtaaacat cactttttta ctcattcaat aataccctat
     4621 taataaatcc tcaaactaat ttaaataaac tttgcataaa attaaaaata tattatataa
     4681 ataatttaaa accacactaa cttattgaaa attctaatta ttacaaataa atattgctct
     4741 ctgttaacgc tagttctata atataattat aagctattaa tattataaag acgatattac
     4801 taatataata attataatac ttatagttaa tttacttttg ttgaaaggtg gtaaataaca
     4861 tgaaaatttc agaagtcgct actccaaatt ttttgctaga tttagaccag ttagaaaaga
     4921 atatagataa aattcaacaa atttgtacaa ataataataa acaactttgg cctatgctta
     4981 agacacataa aagtacttac atagcgagac ttcaaaaaaa tgcaggtgct agtggttttc
     5041 tagttggaac acttgatgaa ggtgaagctc tgataaaagc aggttttgaa gatattatgt
     5101 tggcttatcc ttacatggga gaagtaaata taaatagaat tgtgactatg gcatcaaaaa
     5161 taaatttaat ctgtgctaca gataatatag aatgtgctaa ggcttatagt gaagcttttt
     5221 ctaaatcaaa aatagagtgc aagctactta ttattgtaga ttgtggctta caccgttttg
     5281 gggttgaacc agagaaagtg gttgaacttg ctactgaaat ttctaagcta gaaaacataa
     5341 agattgttgg tataagttcg catccaggtc aagtgtatgg ttgtagttct ccagaaggtg
     5401 ttccagatgt ttgtgttcaa gaagattctt ctatggagaa agcatacaaa gctctaactg
     5461 ataatggatt taatatagaa attgtcgctt ctggtagtac tccaacagtt gaatctgaag
     5521 ctgcttctaa aactataact gctgtaagac ctggaaacta tgtatactat gatgctatac
     5581 agataggttt aggttgtgct actgaagata tgtgtgctct tactgttgtg tgcactgtaa
     5641 tctcaaaaaa tagcagtgga tactatttaa ttgattgtgg aagtaagtgc cttggacttg
     5701 ataaaggtgc acatggaaat agtagtattg ttggttatgg tagggtatgt ggacatccag
     5761 aacttactat tgattcactt tcagaagaag ttggaaaaat taaagtaaat ggtgaaactg
     5821 atataaaaat tggtgataga ataagaatta tacctaatca ttcttgttcc actgcaaact
     5881 tgacaagtaa tttattaggt tttagaggag atacaataga aaaagtcata gaagtagata
     5941 ttcgtgaaaa ttcaaaacaa atcatccttt aaaaacagat ttattctgat attaacatga
     6001 aagtatattt ttcttaaaat tatttctaat aactaatgtt aatgttggat aaataaaaaa
     6061 cataaattct agaaatataa atttaaaaga agaggctgtc tcaaaataga gataagcctc
     6121 tctttcattg ttgaaattat gaaaatatac ttgatttcat acaaaaagag tatctcatga
     6181 actaaaaagt tcactttgag atactctctt tttttaatta atatacacat taattactat
     6241 cttagtttat tactatatat caattactac tcattaatcc ttgtatatat taattcttat
     6301 atatcattta tagttaggtt ataattttat gttatttaca ttaatcccat aacagtgagt
     6361 aaaactctta ctattaacat ccccactatt gcatcaaata taggaactgc caacagtaac
     6421 attctatact tagtactggc atttgctaca agcactattc tggcataatg accaactaga
     6481 gtacccatta acatacatgg cataattaat atggttgctt gtggtgcagt gattagacct
     6541 tgtgcaaata agtttgcagc tgtagctgct cctgctgcct tagcaaagaa tgctgctatc
     6601 aaaactaaaa cagattcccc aggtagtcca aaaataccca ttactggtcc aaaaatattt
     6661 cctaaaatat caactaaacc agttaattgt aaaaactgca ctattacata acccattatc
     6721 atggctggta atatctgttc aacacctatg taaaaacctt tttttgctcc ccccataaat
     6781 atctcaacag cagttttctt aggtttacta tttacaattg catttccagt attttgttga
     6841 ctcatactac acagcctcct tagccttatt ctttttattt cttacaccta tgattaatct
     6901 aactagatta gcaccaagta ttttacagaa tatctctata aggattattg gacctaatgc
     6961 caataatgct attggtaaaa gtggagcttg agtatttatt gtgttcagta taacagctga
     7021 accagcatat tgatatgcta caaatatagt tctttcatca tctgttattt ccccactttc
     7081 aaataattct ttcgtcatta ttgaggctac atctgaacta gtaaatgaag atacaaatgc
     7141 tattcctgct attccaggta taccaagcag aggtctaagc aatggattaa atatctttga
     7201 tgccgctttc attcctccca tagattcaac tacatctata agacctacag caaaacagac
     7261 agttggaatc aaagtcagag caaataagaa accatctttt gctcccgttc ctcctttacc
     7321 cataaagtct acaccatctg caatagtacc aaatgaacct gtcagattag caaaatctaa
     7381 tgctttaagg aagccttgac tatctttaaa cactcctgaa aataatacta tcaatacaaa
     7441 taaagataaa taccctttcc atgttacctt attatctcca gctgttacat ccatataaaa
     7501 tcccccttta tacctcaaat ttatctaagt ttaaaataac tgtagaaatt aatttcaccc
     7561 tctcaaacat agaatctact actgcatatt ctcttgatgt gtggttccat tcccctctta
     7621 caccaaatga acagattgcg ggaacgtttg caatagtcaa ataactagca tctgaactac
     7681 ctccaagtct cttagcattt aattctccaa atccattttc ttttgacact tcatttacaa
     7741 atgtatgaaa tctcataaca tcttctgtag tttcaaatgg catcatttca cttataaaat
     7801 taacttctgt agaagttcct tctatgtaag tttctttaca tatctctcta acttgttctt
     7861 taacattctc catttcttct actttttcaa atctaaggtc tacttttata ctacaatctt
     7921 ctggaataga attttccact cttccaccct taatcacact tacacttact gtagttccaa
     7981 catctaaatt agtcaatttt tgtatgcgta gtatcttatt agccatttct tctatagcat
     8041 ttcttccacc ttcaaaatca ttacctgcgt gtgtttctac accttttaca tgtatattac
     8101 atcctattct tccttttcta cctacacaca aactattatc tactaatcca gtttccatat
     8161 taaaagcaca aagaccacct tttgcttccc tcaatataac atctgcacct gttgaatcct
     8221 tatgtcctat ctcttcatct ccagaaaata ctatttttat tggtctttct ttgtatccta
     8281 ttttatttaa tgctttaata acatataagg aaattattat accacccttc atatctaaaa
     8341 cacctgggcc atatgctttt ccttctatta ttttaaatgg attctctcca aatgtacctg
     8401 tttcaaagac agtatccatg tgacctgaaa aaattatagg ctttttattt atattagaac
     8461 caagtgtacc tatcaatgta ctaccattat ctccaacatc tactaactcg caatctaaac
     8521 cttctttttc aaactcaaac tttaatctct tagccaattt atttacgctt tctttacaat
     8581 gtgtatagct ttctatatta acaatctctt tccataatga aactatttct tctctattct
     8641 catcaacaaa cttagaaatt tcatttaaat atgtattcat aatcttctcc tttattttca
     8701 aataaaatgt atttctttcc taaaatctaa gtttatttta ggaaatctta aaagactagt
     8761 ccttaattac aaaatagcac aattattctt gacagtcaac atatttttca gtctttttta
     8821 tattttctga tttttcttta aattttcctt ttgtaaaata aattgacgcg ttttatctac
     8881 tattataaaa tgaggttata aattgattga ttagttacta tactctcgaa attttaaagg
     8941 aggttttatg aaatataaaa ttggtttttt gacaacatct tattttatgt cagataagtt
     9001 taaacaagct cttaaagaat taaatgatac ttgtgaaatt acatttattg agctcaaagg
     9061 ggagaatcct tttaataatt tacctaatat atactatgag caagtagata attttgatgg
     9121 atttgtatgc agtggtatag ttccatatag tgaacttata ataaatataa aagacataaa
     9181 agttccatta aattttttaa agcttgatga aagagatttt tataaatact tattcaaatt
     9241 attaaatagc caaaaagata ttgactttac aaaatcattt atggattttt taagagagga
     9301 caacaattat tatgatatat attcattaat agactctaat aaatgtccat atacaattaa
     9361 agattttaat atccctcaac ctgtatatga ctttaaaaaa ttaaatgata aattattgga
     9421 aatacattta gacttattgc gagataataa gattaacctt tcttttacta gaaattacat
     9481 cattagttgt gaacttaata aacttggata tgattgtatt tacatggttc catctacaga
     9541 atcaatcctg aatacattta aatctctggt gaatgaaata acattacaaa acttagacaa
     9601 aaataaatct gcaacttgta tagttacagt aaatagttca gagtacataa atgaagattt
     9661 gatatttaaa aatgatgaga tacaaaatca aatatataac actatattaa attctctatc
     9721 aagacatgga ttttatggag ttcaggtcaa aaaaaatgat atgaaaatag agatacacac
     9781 tactaaagaa atgttaaaca atatgaccaa taactataca gattttttta tttcagaaga
     9841 tttaaaagaa attaaatata gcttaaatat aggttggggc attggaaata caaatattca
     9901 tgcagagcaa aatgctcgtc aagctaatgg taaatctgct tctttgaatg gaaactgtac
     9961 ttttatagta aatgatacta atgatacaat aggcccttta tattcaaata aaaattctaa
    10021 taatattgaa gatagctcaa tagccaataa agttgcttct tttatacctc tatctaatac
    10081 aaatgtatct aaaatcatgt gtatgattaa tgatagaaat tcaaataatg tgtcagcaga
    10141 aatattatca aactatatga acataactct tagaagtgct aatagaatat tgagtatctt
    10201 atataaagca ggaattgcaa ctattgtaaa tacaaaactc gataatcaaa gaggaaggcc
    10261 caaaaaaata tataaagttg atttcttatc ttttctaaat aagatgcaga aattaaattc
    10321 ttaatattta attttttaat aagaacaatg tattttctct aattttaaag aatcaaaatg
    10381 tttaaatttt attagaatat acaacttttt actttaaaat acctaaacct tttatcagtg
    10441 aaataaacca taatatatcg taacttatta gaaaaacaaa ataa
//
LOCUS       DBGJFU010000129        10309 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.130, whole genome shotgun sequence.
ACCESSION   DBGJFU010000129 DBGJFU010000000
VERSION     DBGJFU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.130"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            180..1079
                     /locus_tag="ACOIAS_003096"
     CDS             180..1079
                     /locus_tag="ACOIAS_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGZ2702787.1"
                     /translation="MSIVLNMPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKN
                     NKINFEAVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLV
                     GNYIAERYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVN
                     QKLSEWTSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLF
                     RYGGDEFLFLLQNTSCVEAEKIMININYELSKISNTDEYPYALSISYGLVEWDGISDI
                     ETLISKADSLMYENKLKNKSTLN"
     gene            1279..1437
                     /locus_tag="ACOIAS_003097"
                     /pseudo
     CDS             1279..1437
                     /locus_tag="ACOIAS_003097"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(1697..4264)
                     /locus_tag="ACOIAS_003098"
     CDS             complement(1697..4264)
                     /locus_tag="ACOIAS_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HGZ2702788.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(4267..4872)
                     /locus_tag="ACOIAS_003099"
     CDS             complement(4267..4872)
                     /locus_tag="ACOIAS_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2702789.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5246..6706)
                     /locus_tag="ACOIAS_003100"
     CDS             complement(5246..6706)
                     /locus_tag="ACOIAS_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HGZ2702790.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7068..7646
                     /locus_tag="ACOIAS_003101"
     CDS             7068..7646
                     /locus_tag="ACOIAS_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2702791.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            7697..7930
                     /locus_tag="ACOIAS_003102"
     CDS             7697..7930
                     /locus_tag="ACOIAS_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702792.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIINILIILDR"
     gene            7939..9189
                     /locus_tag="ACOIAS_003103"
     CDS             7939..9189
                     /locus_tag="ACOIAS_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2702793.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9350..9919
                     /locus_tag="ACOIAS_003104"
     CDS             9350..9919
                     /locus_tag="ACOIAS_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGZ2702794.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3457 a         1765 c         1500 g         3587 t
ORIGIN      
        1 taatttttat tatttgtact tatttaaagt ttcatctaat tataaattgg acaattttta
       61 tgttgatttt attcatacaa attaaagtaa cttataaaat ctccacaata aaaaacattt
      121 tttattcact aaactgctcc atcattggtt tagcaataaa tatatttttt aggtctttaa
      181 tgtcaatagt gttaaatatg ccagcatatt tatttgatag taattctatt gcaaatattt
      241 acagagtatt tcctatatca tttagcttta tagttacagg actatatttc agttataaaa
      301 ataataaaaa taataagatt aactttgagg cagttttaga ggacaaaaaa aacttaattt
      361 tcttattgag ccttatgttt ggaatgtact tgtatctatg tttgagtttg tcaatatact
      421 atactgttgg aaatgtattg attttaaaat tatatggaat caagtctgcc ataatagttt
      481 tggtaggtaa ctatattgca gaaagatata gctttagaat tactcaaata aaaggttatg
      541 atattaaaaa tcaaatagaa tatgtagaaa gtgtaaagac agagtatgag attaataaat
      601 tgaatgatat tgtatataca gatttactga caggtttcta caatagacct tttgtgaatc
      661 aaaaactatc agaatggaca tcaaatcact acaaatttac cttatgtttt actgatttaa
      721 atagcttaaa acctgtaaat gataaatttg gacaccaatt tggtgataaa tacatatcca
      781 ctactgctca aataataaaa aaacttataa atgtagaaaa ttccttacta tttagatatg
      841 gtggagatga atttttattc cttttacaga atacaagttg tgtagaagca gaaaagataa
      901 tgattaatat aaattatgaa ttatcaaaaa taagtaatac cgacgagtat ccttatgctc
      961 tttccataag ctatggatta gttgagtggg atggaatatc cgatattgaa actttaatat
     1021 caaaggcaga ttctttgatg tatgaaaata agcttaaaaa taaatctact ttaaactagc
     1081 tagttcagat attctacaaa atatatatta taagatagta ctaagaatgt caaaatatat
     1141 agattttgca aatattgaat acagtttact aatgaatgca ctggatgtaa gtgttagtaa
     1201 acacctatta gatgaacact ttactgttgt ttgggctaat gaccgttatt atgaaatgtt
     1261 cggctatacc tagctaaaaa gttaaaaatg accactgtag ccgagggaat agagactagt
     1321 tttcaaaaag atttcttgca agaaatagac tgtgatatgt tacaaggata cgtgttctct
     1381 agacctttac caataaaaga ctttgaaaaa cttatgtttc aaaactcctc caattaacaa
     1441 tataaaattg cttattataa gtgatattat tgtatcaaca aataaaggaa ttgtctcaaa
     1501 attgattttt tctaatcaaa tttttatttt gagacattct catttttttg cataagaaaa
     1561 tacccaccaa gctacggttt ttcttaccgc tgttcggtgg gtattagtag tattatgaaa
     1621 tctgaaaact ttaagtatta ctacttccgc tagcgcaatc acaaaggtcg aaagcttcaa
     1681 agcgaaaata ggctttttac tcatcaaacg tcaccgtacc tgccaacccg tccaccgtta
     1741 tcatttgtcc gctcttaata gctcgtgtgg caatgtgtgt agccataacg gctgggatgc
     1801 catattcacg tgcgacaatg cttgaatggc taagaggacc accaatgtcc gttacaatcg
     1861 cacttgcaga tgaaaataat ggtgtccatg ctggagttgt ggtaactgca accaaaacac
     1921 ttccaggttg aaagctttca aaatctgctg gactattcag tacacaagct ggagccgtca
     1981 caacaccagt actggtacca atgcccgtca acacaatttt cccatctttc tgcttctgtt
     2041 gtgcttgtga tatagttttt ttgtttttct ctggcaactt ggatggtgac acataaccca
     2101 tgtatttttt aagttcagct tttcgaacag gtattatccc gttcatatca gatagagact
     2161 tgtttttatc caactgctct ataagtacct ccaattccga ttttgtgagc caataaatat
     2221 catctaaatg tgaaatagct cctcctcgta tgaatcgttc tgagatttcc tgaaacatac
     2281 aacgaatcag aggatgtccc attcccatca aataaatagc attttcacgc ataggagagg
     2341 tttcctgtgc ccaatggagc aacttaaaaa atagtttttt acgcaatcca cctatgtgtt
     2401 gtaaaatctc ttcttctgca tgttttctac gcttttcaaa cgtggtctgg cgtaaaaaag
     2461 gactttcccc ttttccttcc acaaatgttt ttacggattc aaaagttggt gtaagtgttt
     2521 cctgcggtgt ggaatgcgca aaatcaaact cataagcggt acgaccaaac tctttaaagt
     2581 acttattgat tcgatttttc cattctatcc atacttcctg tgaaactcca acaggcacaa
     2641 ctgaagacat gaaatcttct gctatcttta ttgcaggact gttttgcaga taaaggttga
     2701 tgctgttatt ttgctttacc cattctgaaa taccccacaa ctccttttcc gattgcagaa
     2761 caatcgtatc aaacccaagc aggaaaacgg aagtatccgt tataccagca cgtcgaactg
     2821 ctccttgaaa taattttgta aataatgttt cactgataga agcagcaggc aatgtaagct
     2881 gaatccttgt gaaataaata catgcggcat aaaatattgt ctgaataccc tccataattt
     2941 gatgggcgtt catagtatgc aggggctttt cttcccattg tttaatagca tcctcatatc
     3001 cttttcgtgc cgtttcccag cgtgcaacgc tgttcataag tgtcctacgt aaagagcgtg
     3061 gggaaaggga tttgatagca atcaaaagtg gatttgattt agcagaaaga taaacatatc
     3121 cattaataac cgtatatgcc ccgttttttg gtaataattt tttcgcactt ttaccaaaca
     3181 tatctatcca taaaagctct gatgcacggt ttacgatttc aagaccaagc gtggcgaaca
     3241 aaggtgtaac aggatttggc aaatgttctg ccaaacttcc ctttgtgtat acaacatcct
     3301 gttctggcaa tacccattct ggtggcagaa cagtaattgg acgagcctga acaatgtaca
     3361 atttatcttt ttccagtgcc cactctacat ccataggcat ttgataatac ttctcaattt
     3421 tctttccaag ctgtatcagt tgcattacct gattacgagt aagagcatat ttttttctca
     3481 acctttcagg aaccatggtt tcttctgttc cttctgaagt acgaaccgtc ataatctctt
     3541 tatttgccac ttcatacgat ataattcgtt cggagtcctt atccactacg attgtatcgg
     3601 gtgtaactaa gctagaaacc accgcttcac caagccccca tgcagcgtta ataatcattt
     3661 cgctacgtct accattgatt gggtttaacg taaacataat accagatgaa tcggaaaatg
     3721 caagcttttg tactacaaca gcaagtgcga caatctcgtg ctttatatta ttcttcacac
     3781 gataagcaat agcacgagct gtccataagg aagcccagca tctctttaca gagtcaataa
     3841 ctttatcttc accttgtatg ttaagataag tttcctgctg acctgcaaaa gaagcgtcag
     3901 gtaaatcttc agcagtcgca gacgaacgga cagccaccga tatgttccct aatccagcat
     3961 aagccatttt aattgcgtcc gacacttcct gtggcatttc tccattatgg aaaagctccc
     4021 ctattttctt agatacatct tcaagttggc tggtattatt ggaatcaata ccatctagca
     4081 gtttattaat tcgagactga atatggttcg tctcaacaaa aatctggtat gaagcagtcg
     4141 taacatgaaa accgtctggc acagggatgc ctgctgtcaa tagttttgat aaagacatcc
     4201 cttttccacc aacaatttca atggttgctt gtttatgttc cagtggcaag acataataaa
     4261 tcataatcat tcacctccat cattcataat tccgtgcaga aatatattca ttgtttcatc
     4321 caacattttt tccgaactat ccaatggtcg tgacgtgtag ctgattgata acatggagtt
     4381 aattaacaaa cgtgtcacaa gagcggtatc cgttttgcga aagatgcctg tggtcacacc
     4441 attatcaatt accgatttta taatatcttg atatgcataa cgagcatctt taagtctctt
     4501 tcgatactct tcttctaaat aatcagattc tgtatttaac cacatcagta cagttctata
     4561 ctgctctgga aatctaagat gattaagcat gatttttcta aggcactgtt gtggtgaagg
     4621 ttcaccatca ataatcctat gaacttgttg gatagttttt tctatttctt tctcaactgc
     4681 atatacaaca atctcgtctt tcgttttgaa aaaatcgtat agacttgact ttcccatgtt
     4741 ggctaaaact gcaatttctc tcattgatgt tttttggaat ccattttcag ccataagact
     4801 caaagcaaca gcaactattt tttctcggcg ttctgcttgt tgttcgggtg taagcacaat
     4861 tccttttggc atagggcgat acctcctttt agcgaccact ggtcgtttct tttttattta
     4921 tcatacagcg accaacggtc acttgtcaag gttaattttt ttcattgtat atggtactcg
     4981 agataaaaat gttcatttta gaaattgctc tatttaagta gaaataggct tcatcattag
     5041 aaaacaagac tataaatttg agatatttat catgttcaaa tttcatcaat atcaaagttt
     5101 aagatattaa taaaaaaaga attactaaga aataacatat aaagattcaa aaaaggagtt
     5161 gaacttaaaa ttcaattaca aaaaatcaaa tttaagacaa ctcctttatt ctttatctct
     5221 actattcttt atctctattt atttattaca actccctaac atttaccact actgccttag
     5281 gtctagtcat agcctcgatt gaatacttaa caccttgagt tcccatacca gatgccttta
     5341 cacctaaaaa tgggaagtgg tctggacctc tttcagtctt attatttatt tgaacagtac
     5401 caacttcaag tttatcagct atataaaatg ccttatcaat atcacttgta aatactgatg
     5461 attgaagccc atactctgat tgattagcaa tttcaattgc ttgatttata tctttaactc
     5521 ttataattgg aagtactgga ccaaatggtt cttcccaagc aattttcata tcaacattta
     5581 cattatctaa caatgttgga tagaataaat tattttttct aacattgcct gttattaact
     5641 ttgctccttt atgtaatgca tcatcaacca acccttgaac aaaatcagtt gctttattat
     5701 ctataagagg agtgataact acttcatcca ttggatttcc aattgtcaac ttctctacca
     5761 atggctttat ttttccgact aatttatccg ccacactctc ttgtactaaa attctcttta
     5821 ctgccgtaca tctttgtcct gaatatgaat acgctccaga tactatattc ttagctgcaa
     5881 aatctaagtc tgcatcttct aatactatag ccgcatcttt tccaccaagc tccaataata
     5941 atggtaccat tccagatatt ttagatatat gctgaccaac ttcagtacta ccagtaaagt
     6001 taataaaatt aattccttta tgcgttacaa cataatctcc tatctcacta cccctacctg
     6061 taatagtatt aagtactcca tctggtatcc cagcttttct cataatttct accacatgaa
     6121 gtgcgctaat agccccttgt gtagcaggtt ttaatacaac tgcatttcca ccaataagtg
     6181 ctggagcaat ctttgacatt gataaattta caggataatt aaaaggagat atagctaaaa
     6241 cagtacctaa tggaaccctt gaaacataag acatcttatt tcttgttcca ccagggaaat
     6301 tatctccact aatggcttca ccctccatat tttttcccac atcagctgtg tatcttaaaa
     6361 aatctgctgt tctctcaact tctgcccttg cagattttat gtctttggct atttcaagta
     6421 ccagtatatt agctatctca tctatatttt ctaacaataa atcagcagcc ttatgaaata
     6481 tattggctct cttatataca ggcatttcag cccatacttt tatactctct tttgtgtttt
     6541 ttataacctc atctacctca tgttttgtca tagattgtac tttcccaaca agagaattat
     6601 ctattggtga gtaaatttct attggctttc tcgaattaga ttctacccat tttccattta
     6661 ttaaattttt atatacattt tcttttgttt taagttcatt aaacatattt ttattcctct
     6721 ctctgatata actttgtgta tttaaattat agccatttat tcgttatgta aacatttctt
     6781 tttttcataa acgtttacct ataaaaaaat tttaagttaa cgtttttcta cattcttcta
     6841 ttttaataac atactcgctc caaatctata taggtcacac tttaatatca atatactaat
     6901 ataccaatat accaatatac caatatacca atataccaat ataccaatat aaatattata
     6961 aacataaatc acttgacaaa tttataagta gacaatatta tatatctatg acttaaaata
     7021 tttttaaagt catagatatc atatatagat agaaaggatt gataaaaatg gctaaaagtt
     7081 ttacagaaat agaaaaagac aatataagaa aaagactaat ttcagaatgt gaaatcaact
     7141 ggtctaaata tggatacaaa aaaactaata ttgatgaact ttgttcaaaa tcaggaattg
     7201 caaagggttc attctacaca ttctttgatt caaaagaaaa gttatttttt gaagtattaa
     7261 atggaatcca aaacagactt atatcatcag tagatgaaat tttaagtaac atgcctccaa
     7321 aagaaggctt tgcaaaagcc ttaaagatgt tgtatcgatt atatgataaa aacccattct
     7381 tatattcacc aaataatact gattacatca ctttattgaa taagatacct aaagagatgc
     7441 tttcacaact tgaatacagc aatgtacaaa gtttttatag cttaattgaa agacataatc
     7501 tcaagttaaa aatagagaaa gaaaaagcct ttggtgtttg caatgcccta ctgtcaagcc
     7561 tattgctaaa agaagatatt ggatatgact acattgaagt atttgatttt atggtagaca
     7621 atcttgtaga acacatactt gagtaataat ttaaaataaa ttttctaaac atttctttat
     7681 aactaggagg agtaatatga ttgaattaat acaaagacta aatgatatta tttacaaaat
     7741 aaatgataaa ctaaatatcc acacaaaact tgtagaccct agcagtgagc tcatgccact
     7801 ttactctatg ttatctataa tcgtttctat atttctaata atattaggta ttaaagtaag
     7861 aaaaaaattc cttataataa ttgctattat atcaattatt ataaatattt taataatctt
     7921 agataggtag gtacatatat ggtaaaatcc tcgaacactt ttaataaaaa cttcaacttg
     7981 atgatattag gtcaaataat ctcattgttt ggtgcatcaa tacttaaatt tgctttatct
     8041 ttatacatac ttgatataac tggtaaagca gaaatatttg ctactatact agcagtatct
     8101 tcaatcccaa ttatagtatt ctcccctatt ggtggagcta ttgcagatat attcaacaga
     8161 agaaacttaa tggttatttt tgatttttca agtagtctta cagtactaat cttagcagta
     8221 ttcttattca acaataatgg ttcaatatta cttgtaggaa ttattatgac catattatca
     8281 gtcataagca caatgtatca accaactgtt cagtcaagta cacccctttt agttgataat
     8341 gagcatttaa tgaatgcaaa tggtattgta gctggagttg catctcttac aaatattgca
     8401 ggacctgtat taggaggtgt cttgtatggt gtgattggaa ttaatgcaat tatagttata
     8461 agttgtgtaa gtttctttct atctgcaatt atggagatat ttatacagat tccttttatc
     8521 aaacaagagc aaaacagcaa cattataaaa actatattta ctgatataaa ggatggtatt
     8581 gttcatatat gtaaagaaaa tagatttata ctaaaaatac tttttttagc tatagcaata
     8641 aacttatttt taagctctat gttaatagtt ggtatcccct atatagtaaa aatcactatg
     8701 ggagctagta acttcttata tggtctagca gaaggagcaa tttcttttag ttcactggta
     8761 gctgctgtta gtattggtat tttctcaaaa catctaaaaa tcactaattt gcatatttcc
     8821 tttattatat gtgcagttat gttcatacca atggcggttt cagtatatcc atttatatta
     8881 aacacaggat ttccagttcc atttttaatc tttatagtat catctatgct aatatttttt
     8941 acaacgactc ttatctcaat atatgttata acagtcacac aattaaaaac tccaaatgaa
     9001 ctcttaggaa aagtaatgtc aattcttttt gcaggaacag caattgcaat gccccttggt
     9061 caggttatgt atggtaaact ttttgattta ttatcagata gagtgtacat cattatacta
     9121 ggagttggat ttattacatt acttatagca tttgtatcaa agataacatt gaggaacgaa
     9181 aatctttaga tacaaagaat ttagatataa agatttaatt ttcactaatt tctgaattta
     9241 aatgcaaaat tgtgtatcag tatgttgacg ttttagaaat tgattgatat aatcaatgga
     9301 aaataaactt tataaaaatt caaaatattc agctagagga gtgataaaaa tgagttacat
     9361 gccaacttta gaacaagcat gggaaatatt aagaaaatac aataaggaag aatttcatat
     9421 aagacatgct caaattgtat ctggagttat gagatactat gcaaaggagt acgaccctga
     9481 aagagtagaa ttttgggaaa tagttggact tttacatgat ttagattttg aaatgtatcc
     9541 tgatgaacat tgtgttaaac aaagagaatt aatgacagag cttgatttag ataaatcaat
     9601 aatcgattct acaataagtc atggatatgg attaactggt tcagatgtta aaccagagca
     9661 atttatggag aaagtattat ttgctgtgga tgaacttact ggtttaattg gtgctgctgc
     9721 aataatgcgt ccttcaaaga gcgtttctga tttagaatta aaatctgtta agaagaaatt
     9781 taaagataaa tcatttgctg ctggctgttc tcgagacgtt ataagaagtg gtgcagaaat
     9841 gcttggatgg gaacttgata agcttatcca aacaactata gaagctatga gaagcttgat
     9901 tccagagatg gcaatatagc tcctccatac atataccata gaagttacat ctttaaagag
     9961 catttctctt tttgatgcaa cttcttttta tattttgtag aataaaaagt ttaatatttt
    10021 aaactcacta tataatctat aataaatact ataaataata taatgtataa tagtcataaa
    10081 ctttctaacc ttaaaagttt ctaataatca ataaacaatt ctaatataca aatgtcatta
    10141 tatgatttaa ttatatatct tcaatttata tattaaaata aatatatata tattaaattc
    10201 ttatacacca aatttttaca ttaaacttct atgcaccaca tttatacata ttaaattctt
    10261 atatattaaa tttctatgca tcacttttat acatattaaa ttcctatat
//
LOCUS       DBGJFU010000130        10246 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.131, whole genome shotgun sequence.
ACCESSION   DBGJFU010000130 DBGJFU010000000
VERSION     DBGJFU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.131"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..249
                     /locus_tag="ACOIAS_003105"
     CDS             <1..249
                     /locus_tag="ACOIAS_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGZ2702795.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIRTIG
                     FGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSVYKK"
     gene            265..1848
                     /locus_tag="ACOIAS_003106"
     CDS             265..1848
                     /locus_tag="ACOIAS_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2702796.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1876..3204
                     /gene="ssnA"
                     /locus_tag="ACOIAS_003107"
     CDS             1876..3204
                     /gene="ssnA"
                     /locus_tag="ACOIAS_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGZ2702797.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3349..4701
                     /locus_tag="ACOIAS_003108"
     CDS             3349..4701
                     /locus_tag="ACOIAS_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGZ2702798.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4893..6002
                     /locus_tag="ACOIAS_003109"
     CDS             4893..6002
                     /locus_tag="ACOIAS_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2702799.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6285..7676
                     /gene="hydA"
                     /locus_tag="ACOIAS_003110"
     CDS             6285..7676
                     /gene="hydA"
                     /locus_tag="ACOIAS_003110"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HGZ2702800.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7670..10231
                     /gene="xdh"
                     /locus_tag="ACOIAS_003111"
     CDS             7670..10231
                     /gene="xdh"
                     /locus_tag="ACOIAS_003111"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HGZ2702801.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTEDALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENVVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
BASE COUNT         3964 a         1236 c         2137 g         2909 t
ORIGIN      
        1 gaagaagatg aatttgttca agcagcgtat aagggattaa aagaagctgg aatagattca
       61 gaaataactc aatactcatt ctgtacgaat ggaagtcact acgcaggtga ggctggaata
      121 agaactatag gatttggtcc ttctaaggaa aatcttgctc atacaataga tgagtatata
      181 gaattagacc aattattcaa aggtgcagaa ggatattatg gtatattaaa atcagtttat
      241 aaaaaataat taggagatgg ttttgtgaga actttaataa aacatgggct tatagtagat
      301 ggaaataaaa caccagcata tgaaggagat attttaatag aaaacgagaa aatattaaaa
      361 atatcacaag atttaaacga agaagctgac aaagttatag atgcaaaagg tagagttata
      421 tgtccaggtt tcatagatac tcatagccat tcagacctag ttattttagt taatccatat
      481 aatgaggtaa agattagaca gggaattaca acagaagtgt tgggccaaga tggtatatct
      541 atggcccctc tacctcaaga gcatatatct tcttggagaa aaaatttagc aggtctagat
      601 ggtgaaagtg atgaaattga ttggaaatat gaaacaacag agaattattt aaaaatgatg
      661 gattacaatg gggtaggttt aaatgaaact tatttagtac ctcatggaaa cgttagaatg
      721 gaagctatgg gacttgaaga tagacctgct actaaagaag aaattcaaaa aatgtgtgaa
      781 atcacagaga gagaattaaa agcaggagct ataggattgt ctactggact tatatatata
      841 ccttgtgcat actcattgac tgaagaaatt atagagatgt gtaaggtagt agcaaaatat
      901 gacggagtat ttgtagttca ccaaagaagt gaagcagata caattttaac ttcaatggaa
      961 gaaatcatag aaattggaaa acaatcagga gtaaaagttc atttttcaca ttttaaggtt
     1021 tgtggtaaag caaactggaa gtatatacca caagttatag agcttttaga aaaagcagaa
     1081 aaggaaggaa taagagtatc ttttgaccaa tatccatatg cagcaggaag tacaatgctt
     1141 ggagtagttt tacctccttg ggcacattct ggaggaactg ataagttaat agaaagatta
     1201 agtgatgaaa acgaaagagc aaaaatgaaa aaagatatag caaatggaat agaaggctgg
     1261 gataatttta tagaatttgc tggaatagac caaatatttg taacaagcgt aaaaacagaa
     1321 aagaataaag atacaattgg taagagctta ttagaaattg gaaaaatgag aggaaaagac
     1381 ccattagatg caacgtttga tttattaaaa gaagaagaaa atgcagttgg gatggtagat
     1441 ttctatggat tagaagaaca tataatagga ttcatgaaaa gagacgagca aaatgtgtgt
     1501 acagatggtc ttttagcagg taaaccacat ccaagagctt atggttcttt ccctaaaata
     1561 ctaggaagat atgttagaga gttgaatgtg ttaacaatcg aagaagctgt ttataaaatg
     1621 actaaaaagg cagctacaag ctttagtata aaagatagag gcgaattaaa agaaggatat
     1681 tttgcagata tagttatatt tgacaaggat acagttagtg gttgtgatga ttttatcaac
     1741 tctatgcagt accctacagg tatagattac gtaattataa atggaaattg tgttgttgaa
     1801 gaaggaaaat acaataaaat taaagcagga aaagtactta aaaattaaat cttagcttta
     1861 aggaggaata aatttatgat tttagtgggg aatgggaggc taataagcca agattcttta
     1921 aatccctata tggaagatgg atgtgtagta ataaatgata atctaattga agatataggt
     1981 actacagaag aaatgaaagc taaatatcca gtacatgaat ttatagatgc aggcaagaaa
     2041 ataatcatgc cgggtctaat aaattcacat atgcatatat acagctcatt tgctagagga
     2101 atggcagttc caggaaaacc ttctgagaac tttttagaga tactaaataa tttatggtgg
     2161 agacttgaca aacagcttac actggaagat acaaaataca gtgcatatgc aacgtatata
     2221 gaatgtataa aaaatggtgt aacaacagtt tatgaccatc atgcaagtcc aaatgcaatt
     2281 gaaggcagtt tgtttacaat atcagatgtt gcaaaggatt tgggaattag aacgtgtctt
     2341 tgttatgaag tgtctgatag agatggtgga aatacaatcc aacaaggtat caatgaaaac
     2401 gtaaacttta taaaatacgc tcaaaaagat gatacagaca tgataaaagg aatgtttggg
     2461 cttcatgctg catttacact atctgatgat acattaaata aatgtgcttc agagatggct
     2521 ggtcttgata caggttacca tgtacatgtt gctgaaggac tagcagactt acaatataat
     2581 gtagataaat atggaaaaag ggttttagaa agattcaatg attttggagt actaaaagaa
     2641 aaaacaatag cagttcattg tatatatata agtgcagaag aaatcagtct tttaaataaa
     2701 acaaatacat ttgtagttca taatccagaa tctaatatgg gaaatgcagt tggatgttct
     2761 cctgcaatag agttattaaa aaggggagtt acagtaggat taggaactga tggatatact
     2821 agcgatatgt ttgaatcatt aaaagttgcc aatataattc acaaacatca cttaaaagac
     2881 ccaagagttg gatttgaaga atcacaaaca atgttatttg ataacaatag aaaaatagct
     2941 aaagcttatt ataaaaatga cctaggagta cttaaaaaag gtgcttatgc agatgttata
     3001 gtagttgatt atataccaca tactccattg acagaaaata caataggggg acacactatt
     3061 tttggaatga gtggaagaag cgtagataca actattataa atggaaaggt acttatgaaa
     3121 gatagaaagt taataaacat agatgaagaa agtattttag caaaaagtag agaattaagt
     3181 caaaatctat ggaatagaat ttaaataatt gatattaata tacaagtaag aaacaagaat
     3241 ttagatgtta aaaacagagc ttaaacaaaa ataggaattt aaatgaagta tataaataaa
     3301 aaatatacgg aatagaatat taaattaaat atagaaggga gacccaaaat gtctcgagat
     3361 aaaagcccta aaatagctcc agtagatgaa aggttacctg tatccaaatc ttgggtattt
     3421 tcactacagc acgttttagc aatgtgtgca ggagcagtag cagttcctat gatgatagga
     3481 agtgctgcac gactagatca tgctagtatt gtttttttaa taaatgcttg cttgtttatg
     3541 gcaggtatag gaacactcat acaaagtgtt ggtataggaa atattgtagg tgctaaaata
     3601 ccagtaatag aaggaactag ttttgccgct gtggcaggta tgacagcaat agcctcatca
     3661 tatagtgatt cacaagttgc aatacagaca gtatttggct ctgtaatagt agctggattg
     3721 ttttgtatat tacttgctcc agtatttgaa aaattaataa gatttttccc tagtgtggtt
     3781 actggaacag ttgttatggt aataggtatt tcactatttc cagttgggat aaaatggata
     3841 actgacaaca aattggaagc ggttgaacca aactcattag cattagcttt agcagtattg
     3901 gcaataactt tactttcatt taaattctta aaaggaattt ggaatagtac agctatattg
     3961 gtaggtatag tacttggtac actactttcc atggcagttg gaatggcaga ttttagtgca
     4021 gtaaatggag ttggttggct taacttaaat gtacctttaa aatttggaat gcctagattt
     4081 gaaccatctg caatactatc attgtgtttg attatgttgg tacttatgac ggaatcagtt
     4141 ggtaatatga tagctattca tgaaatggta gacaaagaag taacaggtaa aaatattaaa
     4201 agaggtctat ttggagatgg aatatctaca gcattatctg gaatattcaa ttcatttcca
     4261 ataactccat ttggtcaaaa tacaggaata gtaggtctta caggtataaa atcaagattt
     4321 atagcagttt acgcaggggt tatattgcta gtacttggat tcttaccaaa atttgcagca
     4381 ataataggag caatacctaa gcctgttctt ggaggcgtag gatttgcaat gtttggaatg
     4441 gtaatggttg gaggagtaaa aactttaagc aaagtgaaat ttgatggaac taaaaatggg
     4501 gttatagtag cagttagtgt tggtttgtct atgataccac taacaaatcc tgatttctat
     4561 cataatttcc caacttgggt acaaacaata ttccatagtg gaataacaac aggtagttta
     4621 tcagcagtgc ttttaaacat attcttcaat gaactaggtt ctaaaaaaga aaaaaataat
     4681 aaagttgaag aagcaaaata aaaataaaac aagtaaaaat ctatattgac ttaaagtcat
     4741 taagcattaa taaaaataat tcagagatat taataattca acaaaactat cttatatttt
     4801 aaataatata aaaaagtaaa taaataacga atacaaataa aagaatacaa tctaaatatt
     4861 aaaaatattt aatttaaggg agagataata atatggctaa tttaaaagtt aatttatatg
     4921 gaatggaatt tttaaaccca attatgacag cggcaggacc tggagcaaaa gatggtgctc
     4981 tctgtcaaga ggcagcaaaa ggtggagcag gagctatatg tacaaaaact atatctgtat
     5041 taccagctga tgtaccaaga ccatgtatgg caaaaactaa tagtggtttt ttaaacacag
     5101 aattatggtc agagcttcca aaagagcaat ggatagagga agaatacaaa aaggtaaaag
     5161 aaacagggct tccagtaata gtaagtatgg gatattcagc agagcaaata gctgaggttg
     5221 ctcctttagt aaagccttat gcagatgcag ttgaattatc tactcattat gtaggtacag
     5281 acgttgcacc aatagtaaat gctttaaaag ctgcaaaagc tgttttagat gtaccagtat
     5341 ttatgaaaat gagtcctcat actgacattc aaactattgc aaaagctgtt gaagaagcag
     5401 gtgcagatgg acttgtaatg ataaactcat ttggaccatg tatggcaata gatgtaaata
     5461 caggataccc tataatggga agtaaagctg gatacggatg gttatcaggt gcaccaataa
     5521 gacctctagc aataagatgt atatatgaag cttctcaagt tgtaaatata ccaataatcg
     5581 gtgttggtgg aatcacttgt ggaaaagacg tggctgaaat gatgatggct ggagcaagtg
     5641 ctgttcaagt atgtacagaa gcaatcctta aagggccttc tatctatgga aaaattgcta
     5701 aagaattaaa tgaattttta gatgagaatg gatatgctga tgttaatgaa ataaaaggat
     5761 taactcataa gaaaatggca gctagagggt ttagaactca ttcagtagct ccagtagtgg
     5821 atgataacaa gtgtatcaaa tgtaagcaat gtgtaacaag ctgtgtatac gaagctttag
     5881 aagttacaga taagttgaac atagatgcag acaagtgttt tggatgtgga ttatgtgtaa
     5941 ctagatgtcc aaaaggagct ttaagcatct gctacaatca agtagaagct atgaatgagt
     6001 aataattata tagaattttg gtaattaact tagagtaata ggtattagga taataaaata
     6061 tatcagataa acaaacacgc attatgcatt aataatacag tctaaagtta ataattttat
     6121 tatattgatt taactaaata ttagtttagc tgaatattaa tttaattagg tattggttta
     6181 atcttaatat agatttacta cgtataagtg aactaactag attactttaa gttaattttt
     6241 tcatataaaa taaattgata cattacaagt aaggtggagt ctatatggga attgttttaa
     6301 aaggtgggaa agtagtaaca agtgaaaaaa cttactgtgc agatgtaaga atagaagatg
     6361 aaaaaatagt cgatataggt cttgatatat caaaagaaaa tgatgaaata gttgatgtta
     6421 gtgattctta tgttatacca ggaggtatag atacacacac acactttgac ttagatacag
     6481 gcgttactaa gacagcagat aactttgaga caggaacaaa agctgctata gtaggaggaa
     6541 ctactactat attggatttt gctacacaag ctaaaggatg ttctttgaaa gacggattag
     6601 aagaatggca tgaaaaagct aaggataaat catattcaga ttatggcttt catatggcta
     6661 taacagattg gaatgactcg gtttgtaatg aaatggagga tatggtgaaa gagggagtat
     6721 cttcatttaa actgtatatg gcgtataaag gaagccttca agttgatgat ggtgtaatct
     6781 ttgaagcatt gagaaaagca gaagagatag gagggattat tggtttccat tgtgaaaatg
     6841 gggacataat atgtgaactt gtggataaag caaagtcaga aaataattta tctccaaaat
     6901 atcatcaatt aactagacca gctataatgg aggcagaagc tacatcaaga cttatgaaga
     6961 tagctgaagt tgcaaactct aaagtatatg tagttcactt aagctgtaaa gaatccttag
     7021 aggaggtttt aaaagctaag caaagaggtg tggatgtaat agttgaaact tgtcctcaat
     7081 accttctatt aaatgatgaa ttatatgatt tagatggatt tgaaagtgct aagtatgtaa
     7141 tgtcaccacc tttaagaaat aaagaaaata atgaaatatt atggaatgct attttagata
     7201 atcaaataga cactatagga acagaccatt gttcttttaa ttataaggga cagaaggaac
     7261 ttgggataaa cgatttcagt aagataccaa atggttctcc aggtgtagaa cataggatga
     7321 gtcttttata cacatatgga gtctgtaaaa ataaaatatc tatcaacaag atggtagaag
     7381 taacatcaac taatgcagca aagttgtttg gaatgtatcc taataaagga actatagaaa
     7441 taggcagtga tgcagatata ttagtcttaa atccaaacaa aaaatccaaa atagaagcaa
     7501 aaaaacaatt acaaaatgta gattatacac cttatgaagg gtatgaagtg gattgtcaaa
     7561 tagaacatgt ttatttaaga ggaaaagaag tagttagaga tggagaatta atttgtaata
     7621 ctccaagtgg gagatatata aataggcgtc taacaaaggg ggctagagaa tggtagaatt
     7681 tatattaaat ggagagaaag ttgttgcaaa agaaggcaaa aaacttttag acttccttag
     7741 agaagatatg aatataactt cagttaaaaa tggatgttca gaaggtgctt gtggaacatg
     7801 tatggttata atagatggta aagctactaa agcatgtgta tttaaaactg ataaattagc
     7861 tgggaaaaac ataacaacta tagagggatt atcagagaga gaaagagatg tttttgcata
     7921 ttcttttgct agtcaaggag ctgtacaatg tggattttgt attccaggta tggtaattag
     7981 ttcaaaagca cttatagacg ttaataataa tcctactgaa gatgatataa agaaagcttt
     8041 aaaaggtaat atatgtagat gtacagggta tgtaaaaata attaaagctg tcgatttagc
     8101 agcaaagatt tttagagaaa atatagatgt gccaaaagta aactgtaaag gtctagtagg
     8161 tgaaaatctt cctagagttg atgcagttgg caaagcactt ggatattctg aatatgtaga
     8221 tgatatgaga attgaaggta tgatttatgg ttcagcagtt agagctaagt atcctagagc
     8281 tttagttaaa aaaatagata tagaagaagc aaaagcttat ccaggagttg ttgcagttct
     8341 aacagctaaa gatattccag gaactataaa ggtagggcac ttaaagaagg attgggatac
     8401 tttaatacca gaaggtgaaa taactagata cttaggagat ggaatagttt tagtagcagc
     8461 agaaactaga gaagcgttag aagaggcaaa aaagctagtt aagatagatt atgaggaatt
     8521 aacaccatta agtactacag aagatgcgct tagagaagat gctccaaaga tacacgagac
     8581 aggtaatata cttgttaagg aacatttagt aagaggaaac gctaaagaaa agatagaaaa
     8641 ttcaaaatat gttgtaacta aaagatatac tacacctgct acagaacacg catttttaga
     8701 gcctgaatgt gcagtagctg gtccttatga agaggatgga gtaataatct attcaacaga
     8761 ccaaggggtg tttgccactc aacatgaatg tgcagatatg ttagggctac catatgagaa
     8821 ggtaagagtt ataaacaaaa tggtaggagg aggatttggt ggaaaagaag acatgagtgt
     8881 tcaacaccat gcagccttac ttgcatacca tactaagaga tttgtaaaag tacagttaac
     8941 aagaaaagaa agtattattg ttcatccaaa acgacatgcc acagaaatgg aatttacgac
     9001 agcatgtgat gaaaatggat acttaacagg tatgaaggca acgataatat ctgacacagg
     9061 tgcatatgcc tctcttggag gaccagtgct acaaagactg tgtactcatg cagcaggacc
     9121 atataattat caagatattg atataacagg aacagcagta tatactaata accctcctgg
     9181 aggagcattt agaggatttg gagttacaca gagttgtttt gcaactgaat gtaatctaaa
     9241 tttacttgct gaaatggttg gaatatctcc ttgggagata agatataaaa atgcaataag
     9301 acctgggcaa gttttaccaa atggtcaatt tgcagatgaa ggaacagcaa tggcagagac
     9361 actagaagct gtaaaagaag agtatgaaaa gcatccacgt gcaggtatag cttgtgctat
     9421 gaaaaatagt ggggttggag ttggtatacc tgatgttgga agatgtagat tggtgataaa
     9481 agatggcaaa gtccacataa gaacaagcgc ttcttgtata ggtcaagggc ttggtactgt
     9541 tatgactcaa gtagtttgtg aaactgctag cctacttcct gaaaatgtag tgcatgaagt
     9601 tccagatact catttaacac cagactctgg taatactacg gcttcaagac agacagtatt
     9661 tacgggagaa gctactaagg ttgcgtcaaa taagttaaaa gaagctttag actctggcaa
     9721 aactttactt gatttagagg gagaagagtt ttatggagaa tatgaaagta ttacagataa
     9781 aatgggttgt gacaaagaaa atccagtaag tcatgttgct tatggatatg caacacaagt
     9841 tgtaattctg gatgaaaatg gaaaattgga aaaagtagta gcagcacatg atgttggaag
     9901 agctataaat ccaatcagtt tagagggtca aatagaaggt ggagtagtta tgggtcttgg
     9961 atatgctcta accgaagatt acccagtagt taactctgtt cctacagcta agtttggtac
    10021 attaggttta tttaaatcta cagatatacc agaaatacaa gctttaatag ttgaaaagaa
    10081 caaagattta ttggcttatg gggcaaaagg tgtaggagaa atttgtacag ttcctactgc
    10141 accagcagta caaaatgcct attatgtatt tgataaagaa tttagaactg aactaccact
    10201 acaaaatact ccatatagta aaaaaaagta acaaaaaaaa gaaata
//
LOCUS       DBGJFU010000131        10221 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.132, whole genome shotgun sequence.
ACCESSION   DBGJFU010000131 DBGJFU010000000
VERSION     DBGJFU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.132"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..903
                     /locus_tag="ACOIAS_003112"
     CDS             <1..903
                     /locus_tag="ACOIAS_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2702802.1"
                     /translation="PLILDISFLIKEKYKYILFSFVIISSLIISLDKNIYLALESSSI
                     LLIITVLSIYIENENLSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKER
                     NRISREIHDSVGHSLSTTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAIS
                     ELKPVEYENYQSLFKIKELVKSFIKLTNINVKLTISKNTWNLSRNQSITLYRLIQESL
                     SNSSRHGKATEIRIFITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVE
                     FDNSNNGFTIRASFPKFSGGDFFE"
     gene            896..1543
                     /locus_tag="ACOIAS_003113"
     CDS             896..1543
                     /locus_tag="ACOIAS_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGZ2702803.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1672..2607
                     /locus_tag="ACOIAS_003114"
     CDS             1672..2607
                     /locus_tag="ACOIAS_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2702804.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIIEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2621..3718
                     /locus_tag="ACOIAS_003115"
     CDS             2621..3718
                     /locus_tag="ACOIAS_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702805.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3721..4785
                     /locus_tag="ACOIAS_003116"
     CDS             3721..4785
                     /locus_tag="ACOIAS_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702806.1"
                     /translation="MIRLTIANIKRYLKNHNLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNELDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5029..5526
                     /locus_tag="ACOIAS_003117"
     CDS             5029..5526
                     /locus_tag="ACOIAS_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HGZ2702807.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5702..5950)
                     /locus_tag="ACOIAS_003118"
     CDS             complement(5702..5950)
                     /locus_tag="ACOIAS_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702808.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(6016..7032)
                     /locus_tag="ACOIAS_003119"
     CDS             complement(6016..7032)
                     /locus_tag="ACOIAS_003119"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HGZ2702809.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7090..7857)
                     /locus_tag="ACOIAS_003120"
     CDS             complement(7090..7857)
                     /locus_tag="ACOIAS_003120"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGZ2702810.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7925..10114)
                     /locus_tag="ACOIAS_003121"
     CDS             complement(7925..10114)
                     /locus_tag="ACOIAS_003121"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HGZ2702811.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3557 a         1568 c         1184 g         3912 t
ORIGIN      
        1 cctttaatat tagatatatc atttttaata aaggaaaaat ataagtacat attgttctct
       61 tttgttataa tatcttctct tattattagt ttagataaaa atatatattt ggcactggag
      121 tcaagctcta tattgctaat tataactgta ctttctatat atatagaaaa tgagaattta
      181 tcaaaattat acagtcaaaa tctttatgat aagcttagaa tttctaaaga tgaattaaaa
      241 aaagttaatg ctgatttgga aacttatgca agttctatag aagaattagc aattttaaaa
      301 gaaagaaatc gaatatcaag agaaatacat gatagtgtag gacattctct ttctacaaca
      361 ataatacaat taaatgctat agaaaaactc ttaaaagaca aacctctaat ttatgactta
      421 gtacatgaac ttagagaatt tgtaactgaa agttttcaag atgtaagaag ggctatatct
      481 gagttaaaac ctgttgaata tgaaaattac caaagtttat ttaaaatcaa agaattagtt
      541 aaaagcttta ttaagcttac aaatattaat gttaaattaa ctatttcaaa aaatacttgg
      601 aacttatcca gaaatcaatc tataacttta tatagactta tacaggaatc cctttccaac
      661 tcatctaggc atggaaaggc aactgaaatc agaattttta ttacattcaa tacttcaaat
      721 cttataatta ctattagtga taatggtata ggatgtggca atataaaaaa aggaaatgga
      781 cttaatagta tcaatgagag aatatatgaa ttaaatggta aagttgaatt tgataattct
      841 aataatggtt tcacaattag agcttctttt cctaaatttt cagggggtga tttttttgaa
      901 taaaattaaa gttcttatag tagatgacga aaaattaatt agaaaaggtc ttaaaattat
      961 attatcatct tataatgatt tagaaattgt aggagatgct tctaatggat atgaagcttt
     1021 agaattttgt aaaatgaatg atgtagacat agttcttatg gatatacgaa tgaaagtatg
     1081 tgatggggtt ctaggtacaa ggcttataaa ggaatataat aattctataa cattattaat
     1141 tttaacaact tttaatgatg atgaatatat aaaagatgct atgaagtttg gtgcttcagg
     1201 atacctttta aaagatagct ctgatgaagt attgcatgaa gggattcgtt cttcattttt
     1261 tggcaatatt gtgcttgata aaagtgtagc tgaaaaaatc atgacctctg aaaaaacggt
     1321 taaacaagag catctttatg acatgtacaa tttaacagaa aaagaaatat ctataattag
     1381 actaattgca aatggactta ataataaaga aataagtcaa gaactattct tatctgaagg
     1441 tacaataaag aataatataa ctaatatttt agctaaacta gaacttcgtg acagaactca
     1501 actagcaatc tttgctttca aaaataaaat tgtaatagaa taaatctaaa aatataaagt
     1561 gacttaagtc acttttttta gtgacttata gtaatagaat tataattcac aattctttat
     1621 tataatatta tagcttttta acttatacaa aattattttg gagggattac tatggatata
     1681 gtcagagtaa ataatataac aaaaagattt aatgataaat tagtacttga taacatcagc
     1741 ttttctgtta aaaaaggtga gatatttgga cttattggcc caaatggtgc aggtaaatct
     1801 acacttataa atataattac aaatcttatg ttaccaaata gtggtagtat tcaaatcaat
     1861 ggtttagatt tatcaaaaga ttatataaaa gcaaagtcta taatagggtt agttccacaa
     1921 gaattagcaa ttatagaaac tcttactcct tttgataact tagagtactt tggagctttc
     1981 tacggtttaa agggaaaact acttaaagaa agaatcattg aagcattaga agttacaggt
     2041 cttactgaag tcaaaaagaa aaaggtcaaa aaattatctg gtggtatgca aaggagatta
     2101 aatattggaa tagcactctt aaatcatcct aaaatattaa tattggatga accaactgta
     2161 ggtgtagacc cccaatcaag aaatcatatt ttcaatttta taaaagatat atctaaaaaa
     2221 catgaaacta ctgtaatata tacttcccat tatatggaag aagttgaaca cctttgttct
     2281 aaaattttta ttatggatga aggtaaagaa attgcttttg gagataatga ttatttaaaa
     2341 tcactcgtat ctacaaatac aaaattaatt atagaaataa aaaatataaa tgcccaactt
     2401 atctttgatt taaaaaatac taaaggtgtt atatctgttt tagaaaataa ttcattatta
     2461 gaattggata tagataaaaa attacaactt acagatatac tctctgttat tgacaaaaac
     2521 aattcaaaaa taatgaaaat ttcttatgaa gaacctagcc tagaagacgt atttttaaat
     2581 ctaacaggaa aaaatttaag ggactaatgg aggtgatttt atgaaattat acatgagtgt
     2641 aaaaactatg ctaaagagtt taaaaagctc ctttatactt aatttaatat attttttagc
     2701 tttgcctctg attttatctt ggtttcttgg gatggttaca gaaagcatgt ttcaaaaccc
     2761 tataaaaaca gaatctaccc ccattgttat atatgacaaa gataacacaa gattatcaaa
     2821 tgatttgaca aagtatttga aaaatgattt atcttatata ttaacagtta aaaaagatga
     2881 ttctaaggca gaattaaagc ttactatacc taaaggctat gagagtagtt tattaaatga
     2941 aaaatcaaat actctaaaca tagaaaaact tggaactcgt gatgatattg caatattgct
     3001 tcaagatata ttagatactt accatgaaaa attttattta aataattctc agaaaataag
     3061 ttcagaagat ttttctaaat tatttaataa aaattctatt gatacttcta taataaataa
     3121 caatgttaaa cagaactctt atgaatactt tgctttagtt tcactaggat ttcttgtaat
     3181 aatttttata atgaacaata ttttgtcaaa ttatataagt gaatcaaagg gactttcaaa
     3241 aagactttat tctatgccta taactagagt acaattctta atttatgact ttgtaggttt
     3301 atggatttat tcatttatat tcttattatt atatgtttta ttctttagaa tacttggaat
     3361 aacatttaaa ggtaattttg ccatattgct cctactttgt gctttatcat cttattttat
     3421 gacttcaata tctacctttg taaccagctt ttttagtaaa aaatacggta cagcaatagt
     3481 ttatgcatta ctctttttac agacaatttt tggaggtatc ttcagtatga taagtgatac
     3541 atttactaag cttacaagtt tatctcctac ctatttaata ggagaattgt ttagtaatta
     3601 tgaaacattt aaaactatag attcaatagg agatttaatt ttcacatgtt taattatctc
     3661 tacaatatta attgcacttg ctattgtaaa ggaaaaatat aaatggaggg aggtttaata
     3721 atgattagat taactattgc aaatataaaa agatatctaa aaaatcataa tcttttaatt
     3781 aatatggtta tgcttcctat tatactaatt ttttcactta atttttttat caacaactca
     3841 ggaaatcaat caatgtactt ttctccagta gcaattgtat ctgactcttc aggaaagtat
     3901 gaacataagt taataaatag ttctaaatta aaagaaaatt catttagatt aaatgaacta
     3961 gataaggcta tgaatctatt aaaaaataat aaggtttctg ctgtattcgt tttagataaa
     4021 aatttttcaa atagtataga taaattacaa cgacctgtag ttaaatgctt taaaattgaa
     4081 aatggtggtg gtagtctttg ggcagaatca caaatagaat cttttattac taaatcgtta
     4141 aaattgaaag tggataaaaa tatagatgat aaattgacaa agacaaatat aattgataac
     4201 aaaccagata agaataaagg ttcattttta gtagtcttct tgatatgcta ttttatgtat
     4261 attaatgcag cacatcttgc ttctgattta tttacattaa aaaaatcaaa tgtgctaaaa
     4321 agacttatct ctacagataa taaagatata aaaataattt tcagtatatt tttaggacta
     4381 ttttttattc aatcaattgt atacactttt gctctattat gtttttcaat tatagaagac
     4441 tttaatttga gtctgaatgt attgatgatt gtactagcaa atagttttgt ttccactgga
     4501 tttgtatttt gggtagctag agtatttaaa aatgaaagtt ctataagtct tatatctaca
     4561 ttttattctt taataggtct agctatatct atctctagtc taataccatc aatggataaa
     4621 ttatccttta tgactaatct ttcaaagttc acaccatttt attggactat agatgctatt
     4681 aaaaacaatg gtagtatttt aataaatata atagctctaa tattaattgg aattatattt
     4741 gtaactgcag gaagtcttaa actaagagat tttgctaaaa actaataatt attaaatata
     4801 attagtatct atttttatat agttgtaaaa gaatatttca acatattaaa ttattttatc
     4861 ttatattttt ctctttagtt tcacacatca ccaagtctaa accttatatt atatattaat
     4921 atttttgact tgtcaagtta tttatgttat aatattaatc attagataat taaagcattg
     4981 tacctaagtt gcaatatcta aaattcaatt tggaggtata actagcatat gtataaaatt
     5041 ggtgaaagtg taatgtatcc aaaagaggga gcctgctctg taaatgatat tgtaacaaag
     5101 aaaataaatc atgagatgca aaaatactat gaattaagtg tcatttttaa tagtaacctt
     5161 aaaatttcga ttccagttct aaatgcagat agaattggca tacgtccagt tatggatgga
     5221 aatgatgttg ataattttat ccaatctatt aataaaacag atggtgtatg gatttttgat
     5281 agaaaagaaa gacttaaact gtaccaagat aaatttcatt caggagatgt ttttgaaatt
     5341 gtaaaattaa tcaaaatgct tatgatacaa gacagttcaa aacaattgtg tagcacagat
     5401 aaggagtttt taaataaagc tcaaaaattt gctttatcag aattagctgc tgctcaatgt
     5461 aaatcctata caatggtatt agaggaaatg aaaaaacata tattaaattc aaagaataca
     5521 aattaaacca gttttttaaa ctggttttat tttttatact aatcatccat gctactttag
     5581 cttattttaa actgcctaat ctaatattaa taaagttatc tcaattagat agatgtattt
     5641 tattcaaata aaaaaaacta agagtaaatc atattactga tttactctta gctaataaaa
     5701 tttaatcttc ttcaagttct tttttctctt gtaataatac acttaattca tttgcttcta
     5761 aaacttgtgc ctctgcaata tcaagtaact cttgttgcca ttccacacat ttatctttgt
     5821 aatcaaaagg tagcgttctg tcaactatgt acttatcaat acagtcctcc gcacttttaa
     5881 ttatttcttt tctttcatca ataaattttt ctaattctac taaggcttct tcataagctt
     5941 ttctactcat ataaattccc cctttgtaca attattctac attaaaatga tttttcctct
     6001 tttttgtata aaattttatt tgtatataac ttttccatca atatctttta tagtttgaca
     6061 tccagttaat atcatagaac tacttagctc tgattgaagt ttatttacat aagtttctac
     6121 accatcagtt gcaccaccaa aagaagcagt cacaaaaggt cttcctatta atactgcatc
     6181 tgcacctaaa cctatcattt ttagtacgtc taaaccagtt ctcactccac catctgcaag
     6241 tattgtaact ttacctttaa cagcatcagc aatttttggt aaaacttcac atgctcctgg
     6301 tgtacagtca agcactcttc ctccatgatt tgatacaact attgcatcca cacctgcttc
     6361 tactgccatt aaagcatctt caacagtcat tatacctttc aatataaatg gtagtttagt
     6421 agactttact aactccttta tttgtttaac atttttagga agtactggtt ttccatgtaa
     6481 tgataaagtt actagaccac aggcatctat atcaactcca actgcaaatg caccagcttc
     6541 ttcagctaat cttatctttt ttataatatt ttcattatcc caaggtttaa taaatacaat
     6601 tccagctcca ttatactttt tgactacatc taaattttcc attaaaaaag catctactgc
     6661 cgtatcacca accattgcat aaataccact attagcacat cctgcaacta ctggttctat
     6721 gtaatctctt tcattaattt ttccacccat atttaaagta gttcctgtta ctggagctgc
     6781 aaatataggt gcactcattt tctttccaaa catctctata gatgtatcag gatttgatac
     6841 atcatgtatt actctcatat ttacttttac tttttctaaa ctcttgctat tttctataaa
     6901 agaagaacca cttcctttac ctcccatgcc aggcacttca cctgcacaag caaccccatt
     6961 acaaacctta caaactttac aacttccatt taaattttct ctagcacttt ttaataattc
     7021 attatattcc atctttaatc ccccattaac tatagtaatt tatattatat acattatatc
     7081 atattatatt tacagtatat ttttcaaaaa actttttctg tggataggag ttatgccata
     7141 tttttcaata gcttcatagt gttctttagt tccatatcct ttgtgagatt taaacccata
     7201 ttctgggtat actctatcat attggtacat tatactatcc ctagtaactt tagcaagtat
     7261 acttgctgct gctattgata tagattttga atctcctttc acaattggat tttgagatat
     7321 gtcaatacct ggtattgttg ctgcatcaac taataaataa tccggtgctt tttttaaaca
     7381 atttatagcc tttttcattg ccatatatgt agcatttaat atattaaatt catctatttc
     7441 ttcattgttt actataccta ttccataatc tagtgcttct tcttttatta cttcaaaaag
     7501 ttcatctctt tttgcctcac tcagtttctt agagtcattt acaccttcta ttttggtatc
     7561 ttttttaaat actacaacag aagctacaac aggtccagca agtggtcctc ttcccgcctc
     7621 atcaattcct ccaatatata aataaccttt atcatagcct tcgttctcaa atatatttat
     7681 agtttccaat ctttcttctt cttttctaat attatccagc ttttttgcta gttttatagc
     7741 tagaccttgt acagattttc tttcatcaac tcttaacagc tctatatatt ccatatattt
     7801 ttctacttct aaagtctcta ttatttcttt aatctccctc acacttttat cctgcatacc
     7861 attccctcat ctttcgtttt taaaaattta atttttaaat atattaaaat tttttaatta
     7921 gagtttaatt gcatccacta tagccacatt ttaaacatat actacatcca cctgtatgtg
     7981 caattccttc acttccacac tcaggacaca caaatttttc atcttcttca aaatttttat
     8041 ttattatttc tttggctttt tcatctttat tatctttttc ttttttatta tttttataat
     8101 attctttttt actattttct tttttatctt ctttattatt ttcttccttt ttttcattta
     8161 ttaaaattcc actcctttta catccatctc taaatattgt tactccttta agaccagcct
     8221 cccatgcctt aatatatata ttgaaaacat cttcaactgt agtttcaaaa ggtatattta
     8281 ttgtagatga tatagaagca tcaatatact tttgccatac tttttgcatg tttattcttt
     8341 cttcatatcc tatattcata gcagtaacaa aaaaatctgg taaacattct tcatctgtaa
     8401 tacctttctt gtccatatac tctttaacaa ttggagtata tactttataa tacacatcct
     8461 catcatgcaa gctttcagtt tttcttatat atgaaagatt aaatataggt tctattccac
     8521 cactaatacc tatcattgta gatatagaac ctgtaggagg tatggttaaa agttgtgaat
     8581 ttctaagccc atacttttta accatttcct tagtgtcttc atttagattt tctataaaaa
     8641 acttagattt agaaatattt tcatatttat actcactaaa tgttccatat tctttagcaa
     8701 ttagtgctga ttgcttcaca gcttcattta acatagtctt accaattaaa tcacatagtt
     8761 ctaaagattc atcagaacca tatcttattc ccatttttat aagcatatca gctataccca
     8821 ttacacctaa acctatctgt ctatacttat gtacactatc tctttgttct tgtaatggat
     8881 gcaagttcaa tccctcatct aagacatcat ttaaagctac tacagcttct ctaacgcaag
     8941 attttaattt attaatatca aaagtagcat gttcaccaaa tggttgtatt ataaactctg
     9001 acaagtttat tgagccaagt agacaacttc ctccacttgg aagtggttct tctgcacaag
     9061 gatttactcc tgaaaagcaa aagttcttat cttcactcat aaaattatat gtttttattt
     9121 tatcccaaaa caatatacct ggttctgcat aatcccagtt atttttcaca aatttattaa
     9181 ataaatcata agcatttact atcttaacta ttttttctcc agtagtttca acttcaaaag
     9241 tacaattata taactttttt tctcttacag cttgcataaa ttcatctgtt actcttacag
     9301 aaatatttgc tttagtaacc ttctccaaat ctgtttttat atctataaag tcttctatct
     9361 ctggatgatt tacatccata gaaatcataa gagctcctct tctccctctt tgaccaatta
     9421 agccagttgt catactgtaa agttccataa aagagactgc tccagtcgta tgttttgcag
     9481 cattgttaac tttcgctcct tttggtctta gctttgaaat atcaattcca actcctccac
     9541 cataactaaa agttctagca agtttttttg cagtatcaaa tatatcttct atgctatctt
     9601 ttggtggttc tattacataa caattagaat atgttacttt ttttccttct ttctgtaatc
     9661 ctctatttgc taaaattctt ccagcaaata gaaatctttt ttgtcttatt agttttgata
     9721 ttcttgaact attatttgaa attctcttta tccattcatc aaaactctca ccattatgtc
     9781 tatacttgtt cttccatatt gatttttgta attcatcaaa ctcccaagct ttagtcctta
     9841 tttcagagcg tctttctcta tataatatgt agtgttttgc tatatctttt ctatttgatt
     9901 ccataagtag tacttcaact atatcttgta tttcttctac ggataagcat ttttccatag
     9961 aattatagtc attagctatt tttcttgata tatcatatgc caactctcta tctactccac
    10021 cttcactatt tatactagct ttatagacag catcttcaat cttactttta ctaaaaagtt
    10081 cttttgtacc atctcttttt attatgtatt tcatttttaa acctccaata aattagtatt
    10141 aaattaatac acacattaac tataactcta aacaaacttt ttatatattt gtatattttc
    10201 atattaatta tctaacataa t
//
LOCUS       DBGJFU010000132        10150 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.133, whole genome shotgun sequence.
ACCESSION   DBGJFU010000132 DBGJFU010000000
VERSION     DBGJFU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.133"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(208..1785)
                     /locus_tag="ACOIAS_003122"
     CDS             complement(208..1785)
                     /locus_tag="ACOIAS_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HGZ2702812.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="ACOIAS_003123"
     CDS             complement(2082..4493)
                     /locus_tag="ACOIAS_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HGZ2702813.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            4720..5115
                     /locus_tag="ACOIAS_003124"
     CDS             4720..5115
                     /locus_tag="ACOIAS_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGZ2702814.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
     gene            complement(5168..7300)
                     /locus_tag="ACOIAS_003125"
     CDS             complement(5168..7300)
                     /locus_tag="ACOIAS_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2702815.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(7333..8040)
                     /locus_tag="ACOIAS_003126"
     CDS             complement(7333..8040)
                     /locus_tag="ACOIAS_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGZ2702816.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            complement(8149..10020)
                     /locus_tag="ACOIAS_003127"
     CDS             complement(8149..10020)
                     /locus_tag="ACOIAS_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2702817.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
BASE COUNT         2956 a         1842 c         1171 g         4181 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
      121 atctataaat ctatttgtat ataaacctgt ttaactacat aactttagaa caattacaac
      181 acacacttca cttaaatata catattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
      301 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatgatt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
      721 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tatctctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 gccaacagct aacgagtcta ttagttgatt ctcattttta ctaccatcct tagttacata
     1021 taaattactt aaatttgtat cagtgtaaaa cttttctata actttagcat tagtctcatt
     1081 tctatctttc ccactcactc tttctgcatt aggaagactc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatccctta taaatttatc
     1201 aaatgcttct actccatcct taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgcccca acgctaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacttttt aacttgttct ctatatcttt
     1441 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctagcactac
     1621 attaggacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaatcctat
     1921 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
     1981 aagttcattt tgagacaccc ctttttgtta ttttatattt ataaacttct attgaacatt
     2041 ttttaataat aaaatgtata tccttactat ctttccattc actattttcc taaaagagta
     2101 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac tagaactact
     2221 ggtgaatcag ttttagcacc caatgctcct actgctagtc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgagctagg gtagaattta
     2341 tctattatct tagcattagt ttcatttcta ttactgcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 attttagatg aatttaaaaa ctcatcagca ccttgcaact cacttttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
     2641 agagatatac tggtcttgta tctatcacta cctgaaattc tttctactga tatacccata
     2701 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
     2821 gtcaaaagaa ttggagagtc aattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcatctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatcct caaacttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatataaat aatatgcatc tatattagtt tcatatgcta aagttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttacc actggtgcat aatatacttc atattttgct
     3361 cctacagagt ctgtttcaaa cataacagaa tcaagttttt cagagtctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
     3481 tggtacattt ttttatcact atctggtttt gatactgatt tcattgcata gttatctgta
     3541 tctgttagta gagttttatc ttcataagaa atccagaaga atcctttcat agaattaaac
     3601 tctccccaac tactttttac taaccatgct ccatttgatt ctggttttac atcagatgca
     3661 aaattatctt ttgaataatt atcatcccat cctactatcg ctacagcgtg atttaatgga
     3721 gctctcttat ttgtacagtt atatgctgtt tcatctttat taaaatatgt tgaataatgt
     3781 gcataacctg atgtcactga accatattgc attatagcat tctttacagt ttccttatct
     3841 ttattaagac gaacaacatc tgttacatta aattgagtag gtgcagtatc catatttgaa
     3901 ggtttagttg caccttgtgc ctcagattta agattatatg gtatgtcctt ttctaactta
     3961 ggacctgccc atgctgtcag atatcctata gctgttacat ttgaactccc tgacatatca
     4021 tctaagttcc atccatattt tccaccagtt gcccaccatc ttaaatgttc ttctgataag
     4081 tcatacgttc catatccttt taatttaaga tatgcttcta acgttgacat acctgaaaaa
     4141 gaccagcatg tattaagact tccttgattt tttgctggtg tagtaagacc aagattcatt
     4201 gggttgtatg ctacacttga tttatatgct gatggaagac tacttctacc cttatttcga
     4261 agtgttgtac cttccatttc atgtggaaaa ggtattactc cattatattt tgctgtgtct
     4321 ccatttttga catcttctag gtattgaaga tagctttcag aatactctgc agtttctact
     4381 ccatctagag ttttatggtt ttctgctgag acaggtattg ttgacactat taaaaagcaa
     4441 actgtcaaca atgctagtag ctttgacaag ttttttgatt tgtactttct catgtttacc
     4501 cccttatatg tatttaattt ttattatatc agatttctaa attaaggtca tattttattt
     4561 tgataaaata tggattatga gtacatatta attatagaat attatagttt tatttttata
     4621 atattttaag atatattgat ttattaatag atgtgttata ctaatgtttg ttagtatctt
     4681 taagtatatt tatattaaat tagcttagga ggattatatt tgattttaaa aaagcataga
     4741 gagacgatat tatatttatt ttttggagca tttactactc ttgtaaatat agtttcgtat
     4801 ttatttttta ctagagttat tttatttaat tttatggttg caaatgcttt ggcatggata
     4861 cttgctgttt tatttgctta tgttactaat aagttttttg tgtttgagag taaaagaatt
     4921 gaactaatat ttttgtttaa ggagtttttg ttttttgtca gttttaggct actttctggt
     4981 attatagaaa tgattattat gtatataatg attgatttat tttttgtaaa tgatattact
     5041 gtaaaagtat ttactaatat aattgttata gttttaaatt atttatttag taaaatgata
     5101 atttttagga aataaaaaca gactgcccat tattaatcag tctgttttta tgtttcacat
     5161 tagataccta ttctactacc ttatcacttg ctccccatct agttacttta ttagttgaat
     5221 catccaattc aactagttct atatatttat cctttacatc gtttgcttct atcctcactc
     5281 tattagtagt aatttcatcc caatcagata tagttacttt atctccaaca ttcattgata
     5341 ctggcttttt tgatactatt ctataataaa ctgtacatcc agaatcagca cctgttgctg
     5401 ttaatttaac ataatctcca tcaaaatcta tatctgcact taatcctttt gcagtttctg
     5461 attttaaata aaatccatca tctatatagt cagatttccc ccatctagat actttcttag
     5521 tatcagaatt tatttcaaca gcttctatat aatatccatt ttctgcttta atagtaaatg
     5581 gtaaaacaga agatggtaca gcagtccagt cacttgtatc tattctatct ccgatatata
     5641 atgcagatgg tctactgctt actactctat aataaatttt ataatttgta tcttcaggct
     5701 tagtgaatat ttttacactt atataaccag ctcttgcagg tgataaaggt tctgaagtag
     5761 gatatattcc acttgctctt ttttcatcct tatcggtatc atcaactcta ggtttatttc
     5821 cacttattgc tggtgcatta attccactta aagtatcaat atttcctgaa tttcttatta
     5881 taacgctagt agaattattt gtaaccttac ttatatcacc tttattttct atatttacat
     5941 ctttagcact actagatata gttaaaaccc atatatctcc accactttta ttctctattt
     6001 tacatccatt tggatcataa atgtcaatat catttatatc accttcattg ataaatgtgc
     6061 cattttttat atccattatt ttcacagttc catctaatgt acccttatta gttaattctc
     6121 catttggcat atttacagtt attgtttgtg tatacaatcc ttcaagctct acagttatag
     6181 ctttctttgt ttctatacta aatgaactag taattttttt attatcatca ggtttaaatg
     6241 ttataatatc attagcatct gcttttctta gagcaacatc caattcgtct gctgtcttaa
     6301 ctctatatat agtttcttct tgtaaatcaa gaatatcctt tacagcatct tcatttccat
     6361 ttccaccaac ttgcacaata ttatcaaaac ttttagtatt taacacatca tattggctag
     6421 agtcaagtga atttcctact aatacaactg gagagctatt tttagctgcc aatacaccta
     6481 cagctagaga atctatcaaa tccccctgtt ttttcatacc atctttagtt atataagcat
     6541 tttttaagtc tgtattttta taaaattctt ctagaacctt tgcattagtt tcatttctat
     6601 tgtttccaga taatcttttt acattaggta aactctgttc aatagaacta gatacagaat
     6661 atgtaccacc aattatataa gatttcttta tatttttact actaataaag ctatttgcaa
     6721 cttttattcc attgttagga tctgaaagta taattggcat attttcttgt gctgcagcag
     6781 aacccacact tactgcatct gctagccctt tctctccatt aactacaact atatcagaaa
     6841 tactcttctc tttatctaat ctttcagcta attttaaaga tgtttcatat ctactattac
     6901 ctgaaatcct ctcaaaagtc atattttctg attttaattg tttttcaata ttggtgctta
     6961 gagtattcat acctccaatt aaatatacat tttttacacc tagtcttttt aattctgctt
     7021 tagttctact atccaattta tcactttgag ttaataaaat aggtgcatct tttaacttag
     7081 caaatggagt tgcagataat gcatcagcta tagaattatc atttactagt atagcattag
     7141 tagcatcttt ccatccattc tgactaattt ttattgcagt ttcccatcta ccagagccca
     7201 ctagactctc ttttgatgaa gcatcagcta tattgataga taatgcaaag ataaaaaaag
     7261 ttgctgtcaa caacgacatt attctcttag atattttcat taactatatc ctcctagttt
     7321 ttatattaat gtttatggag catgtacata ttggatttta ttggatttta attcatcaaa
     7381 gtgagatttt acagaatgat atccaattgc gtagttacca ttttcaggat tccatttctt
     7441 gtatgcatcc atagcattta ataatttctc ttcttgacta ctcaaaacct tccatactct
     7501 aacatttgga ttaacttttt taaaatcatt ataatcgtat ggctctacat aaaatcttaa
     7561 atcttttatt tttccttctc tatacaattc taatgcagct tcacctaatg ctctatggtc
     7621 ttcatgtatt ccaagagctt tatatggtgt aacggtcttc actttagcat caggatattt
     7681 ttctaaatat tttagcatta tatctcttgc tttttcctta ctcaattctc catcatgagc
     7741 tctattattt tctatatgta tattattttc tttaactccc attgctttac aactattttt
     7801 aaattcttca tctctatttt ttgaaaaatc actttctgat aaagagtact tgtgtacatc
     7861 tttaatatga taagaacatg accctccatc atctaatact tttcttatta ttgatttact
     7921 gccatcagta cataatataa catgtgtatc gcttctttct acatgttcaa ttattgcact
     7981 acccatactt aatgtttcat catcttggtg tggggaaaaa aacaatgttg cttctatcac
     8041 tatcatatcc tcctatctgt caagagcatg ttatacttta atgaatatta tgtcgataga
     8101 aaaagattcc tataaataat aaggaatcct tttctatatt attgattatt accatcctag
     8161 tattttgcct attttttgaa ctacagtcaa ttcaacccca ttacctattt gagttacttc
     8221 tgcaccaaga tttagttttt tatctaaagt attcttttgt gagtttgtta tatcattttt
     8281 agctaaaaga attggtcctt ttcctgctgc tagaggacct gctgccaatg catctactaa
     8341 cataccatta tttccatatc catcttttgc tatatatagt ttatctaatt tgccataata
     8401 tgtttctata actttagcat tagtatcgtg tcttgtttca ccttcaactc tttgaacctt
     8461 tctatcttta tcaatatcag caagttgatt ttcaatttgg ctaggtacat tattagaacc
     8521 accaactata ccaatctctt ttccatccat caacttgata gcatctgctg taagatcatt
     8581 ccaaccattt actattatag gagccttaag ttcagcagct ttagcagcta tactcatagc
     8641 atcagcttcc ccttttgcac ctactacaaa tgcttcttta aaagaaccca tagcttttgc
     8701 tactgctact gaagttgtat gtctatcatc tccagccaat ctttcaacat tcttagttac
     8761 tgattctaat tgctttctag ctgtatttga tactgctgat tctccaccta ctatatatat
     8821 tttagctgat ggactttttt taattatttc ctttatataa tctaaagtta cttttggtat
     8881 ttcattatca gacgccaaca atataggagc ttttttagat tgagctagtg gtgttgctgc
     8941 taatccatca actaatttat ttgaattaac tataactata ttgtcagtat attctgtttc
     9001 ttttgctata gctacagcag ttgcatacct gtcttcccca gctattaaat tgacttttgt
     9061 atttccattt aaggcatctt taacatcttt taactcttta tcattagtac tttctacagt
     9121 aagttctatc gcttttccag atgtatcagt atatttaaca gtaaatttat actttcctgt
     9181 ctctgaatct tttgatgaaa tatcactaat tgattttcct acactatttg ctattgtatt
     9241 acctcgaata gtcaatctat atccatcata taaatcttga ggtgaaactg tatttaaatc
     9301 actatttttt atagttattt cagctaattg aatatcagca atatttttag ctccttctac
     9361 acctaattta tttattttac ttactctaaa tccttctgca gaagggtcta aattggttcc
     9421 tgctatatca actggatttg ctgttaaatc aaccttttca tcaccaatac ctatttgtag
     9481 cgttttagaa gttactacat cagtcttatc ttttaaagtt attactaact tatcttttgt
     9541 actgtcatat gaagcttcta cagttgccac tccataaatc ccagtagctt cagttttagc
     9601 tttttctgtt attgaattag cttcagctga aaggtctgct gaattttcat atttttcaat
     9661 tgcttcagca accacttcat ttgcactatt tgttgtatgt cctttatctg ctatagtcac
     9721 aagaagctct ttattttctc ctaaagcatt tactaatttc tctaattcgt ctatattagt
     9781 tattattttt aattgtgatg aagtttcacc aacttttatt tcatatattg gttgtccaac
     9841 ttgagaacct cctgtatatc tctgagccat tagttctcta actttagaaa ctaattctgt
     9901 tgcttccttt ttagttattg tttgtttctt tacttgtgta gcttctgcag caaaacttgg
     9961 agctactgat gtacttacca ttgcagctgc cataattaca taaagatttt ttctattcat
    10021 atatttcccc ctacaaaatt tggttttcac catttttcct tatttaccaa ttataatata
    10081 atttacacat tatttcaata aactgtagat attgtaatat ttggatattt tattaataaa
    10141 aattaacaaa
//
LOCUS       DBGJFU010000133        10058 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.134, whole genome shotgun sequence.
ACCESSION   DBGJFU010000133 DBGJFU010000000
VERSION     DBGJFU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.134"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..1713)
                     /locus_tag="ACOIAS_003128"
     CDS             complement(<1..1713)
                     /locus_tag="ACOIAS_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HGZ2702818.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIE"
     gene            complement(1931..2821)
                     /locus_tag="ACOIAS_003129"
     CDS             complement(1931..2821)
                     /locus_tag="ACOIAS_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2702819.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            2970..3830
                     /locus_tag="ACOIAS_003130"
     CDS             2970..3830
                     /locus_tag="ACOIAS_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2702820.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            4286..4852
                     /locus_tag="ACOIAS_003131"
     CDS             4286..4852
                     /locus_tag="ACOIAS_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2702821.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            4892..5473
                     /locus_tag="ACOIAS_003132"
     CDS             4892..5473
                     /locus_tag="ACOIAS_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2702822.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(5530..6447)
                     /locus_tag="ACOIAS_003133"
     CDS             complement(5530..6447)
                     /locus_tag="ACOIAS_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2702823.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            6577..8208
                     /locus_tag="ACOIAS_003134"
     CDS             6577..8208
                     /locus_tag="ACOIAS_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2702824.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFVRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            8211..9158
                     /locus_tag="ACOIAS_003135"
     CDS             8211..9158
                     /locus_tag="ACOIAS_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGZ2702825.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(9443..9706)
                     /locus_tag="ACOIAS_003136"
     CDS             complement(9443..9706)
                     /locus_tag="ACOIAS_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HGZ2702826.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(9805..>10058)
                     /locus_tag="ACOIAS_003137"
     CDS             complement(9805..>10058)
                     /locus_tag="ACOIAS_003137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702827.1"
                     /translation="IALFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSD
                     SGYYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         3514 a         1445 c         1419 g         3680 t
ORIGIN      
        1 ttcaataatt ggttgtccat ttcctaaaat ataatcttta gaccaacctt ctggcaattt
       61 atttatccca tttccaatta acttcaagtg taaatttgat ggttcttttt tcagatttaa
      121 tttaaatatt ccatcaaaag tatctttatt atcgcttcca aataaactta tattgccatt
      181 tatatttgta gctgttacat ttacaattcc attgtttgct ctaaatttta tttcagatga
      241 tatagtttca gctgttacac ctgaaatccc actattagta tttatattga aaggatttgt
      301 tacattgtta tcaacaatgt tagcaattcc gtcattgaca tcaacagtag ctcctccttt
      361 aatattataa agttctactg aaccactttc aacatctact ttaaaattag aatacatttt
      421 atcgggaact ccaattttta tcacatttac catcctatct ttatttgcat ttatcatatt
      481 gtcgccattc atatcttgca aatcactatc tttcttatct atttttttct tagcacttac
      541 aactagcttt ccatttttta tattattaga agaattttct ttatatacag aaaaagtaca
      601 atttttttca ttagttacac ttacgtaatc aaactcaaat ttattggtat tcgtcttttc
      661 aacaaccaca cctaaattat ctgattctat aagtatttca gaaacttcat ttccttcaac
      721 tgaagttaag tgttttaaaa tatttggagt catatccata ttattatatt taatcatatc
      781 taaacactgt ttatatatat tggcatcaat acctccaact tttgttatac ttttagctcc
      841 ttttaaaata cctttattac ttccatcagc aactaaaacg attggttttt ctttggctaa
      901 aggagataat gttagtgcat ctactaattg atatccattt gttatataaa aatctgtagc
      961 tccattataa aacttttcca ttaccttttt attggtctca aatctatcat ttcctccgat
     1021 tctaattgct ttagtttcat ttactaaatc ctcatttatc gcagatttgc cacctatagc
     1081 gtaaacattt aaaccttcag tcttaaatgg aactgatttc ccatctgtaa gtattatagc
     1141 tcctttattt tttgcaacta caggtgctat actcatagca tcagcctctc ctacaaatcc
     1201 atttgtaagc attacctctt ttacactact tacttcattt atttttttag ctacattata
     1261 actagtttca attctattgt taccatttat tctttccact tgaatacctt ttctttttat
     1321 atctttttct actgaatttt caatagtatt ataaccacca attatatata ctttttttac
     1381 aacatttaaa ctcttattat ctagtctatt taaagtagct tttggtattt cacttttttt
     1441 agtcaaaagt attggtgcgt taatagctcc agctaatcca cttgatgaaa gcccatcaga
     1501 taaattttta tctccattaa ctaatattac agtatcataa gtttgtctat ccgctataag
     1561 tcctgctgtt tcatatctat caattcctat tattgaatct tctttaatct gtgcatttgc
     1621 agttaaatta ttaaaaagaa acatagtcaa aactaaaatt aatatactta gttttttact
     1681 gaattttttg aattttttat tcataatata cactattcct cctgtaaatt aaggtcttta
     1741 atttgcttat aaattatccc cttatttaca aataataaca taatattata gtaacgtata
     1801 tattacctta aaattatcag taactttata tcaatcatct tagcttattt atataaattt
     1861 ttttatatta cttttcaaat aacagtcata ctaaaacaat ttcatcatat aatttgaaaa
     1921 ttattactat ttacgcagct tttatcatca tcttaccacc atcaaatata tattcaacca
     1981 tattattctc atataaataa gttatataag aacgtattgt acttccaacc aacacatatt
     2041 gattagcatc aagcttgaca ttgtacaaat cacacatttt ttcaataact tcatcaatca
     2101 taactggctc ataacaaacc tttttaatat tatctattat ttcttccatc ttcttcttat
     2161 taatattaat taaacttttt atatctgtag ttttgacaga gtgacttgga acaaagaaat
     2221 cagcctctaa cattctcaat ttttctagtg tcttaaactg tgaatctata tccagtagga
     2281 agaaaaaatg atatttagta attatattct ctggtgtaag actatctgca acaaacaaaa
     2341 cattatctgg agttctaaca ccaatcattt caaaataatg acctggaaga gaaatcgctt
     2401 caagttctgt atctacaatt ttgccatttg aaggtataat ataatcaact tcactttcct
     2461 ttgctaataa aaatttattc ttcagtgctt tacttggata gccaccatat aaaaatgctg
     2521 attctaatat tggattttct gtaaaatatc cttcaatttt agtcgtagca atcttgcatt
     2581 ttgtttgatt cttcaaatat gcatttcctc ctatatggtc tgcatttgaa tgagtgttta
     2641 ttattaattt aggatttaaa ttatgttctt ctaataatct taaaacttgt ctagctgtat
     2701 ccttattatt tccactgtca attaaaattg catccttacc atctacgtat acacctacta
     2761 ttgtagggag tggaatataa tatgtatttc cttttatatg ttctagagtt aatttagcca
     2821 ttttataacc tccctttaaa tttactttta attatagcat taattttctg aaaattccaa
     2881 tcaaattgtt tttataatat atttttattt cttttattaa aaattggaaa aactatataa
     2941 tatatattat aatctatata taaggagtga tgttattgaa aaaagataga ataacttttt
     3001 cagaaatttt aatattaatt gtaggctgta tattaatggc catttctctt aacttatttt
     3061 ttaatccaca tgcaatcgct gctggtggta taacaggact tggagttgtt ttaaattcat
     3121 tattcggtgt agaactttgg attgtcaatt tacttttaaa tgttccatta ttcatatttg
     3181 cttacaaaat actttcaaaa aaagattgtt ttaaaacggt tttaggaata atttttctta
     3241 ccatagcact taagcttact gcaaatatgg ctacattaga tataacaaat gatatgtatc
     3301 ttgcaattat atctggttct attttaatgg gagttggtca aggacttatt tttagaataa
     3361 atggctctac aggtggaact gatttaatgg cactcctact aaataaatat tttcctacat
     3421 ttagtatacc tgttttaatg ggtattgtag actgcgttgt agttgtttta tcaggtatag
     3481 taaatagaca ggttgaaatt gcactttatt caactgtggc tttatacatt ttagtaaaag
     3541 ttagtgactt gcttattgaa ggatttaatt attccaaatc attcactatt atatctgacc
     3601 tttcaaaaga tataagtaaa aaaataatgg aagatttaga tagaggtgca actattctaa
     3661 aaggagaagg tgcatatact ggagaaaata aaaatgtact tttagtagta gttgaaaaga
     3721 aagaagttgt cgaattgaaa aaattagtaa aaaatgtcga ccctaatgct tttattataa
     3781 ttacagatat acatgaagct ttaggtaatg gatttaaaaa aatagaataa ctaacaaata
     3841 aatagcaagt ataattaaca tatacaaatt caacttttta tatgttatat tatacttgct
     3901 atttatatat tatacttgct atttatatat tataattgtt gtttttaaga taaaaattaa
     3961 atgtatcaat tattgtttta tacaagtatt tccaaatccg ttctcattta gatagtatgc
     4021 ttagaaattt tagaattatt gagtctaact ttattactta ctttacttta taacaatcaa
     4081 atacaaattt aaatattata aaactacaaa ataatttaaa gcatataatg tcaatatgtt
     4141 taaattataa aatattcatg tagttatata ataaataatt tataaatata gccaacttag
     4201 cctaaatacg tatataatat agctaatatg gttcttatac ttgataccat caataaaact
     4261 aaaaatataa aaaggagaac ttaaaatgag tgttagttta caaaaaggac aaagagttag
     4321 cttagtaaaa gataataatc ctgtaaaaaa tctagtagta ggcttaggat gggatatgaa
     4381 taaattgggt aaaaaaaatt atgacctaga tgcatttgca atagcattga ctaaccaaga
     4441 taaaatgaat gttagaggtg acttggttta ttttggaaat cttaaacacc cttctaaagc
     4501 tattatacac actggagata acttaactgg aaaaggtgaa ggtgatgatg aacaaataat
     4561 tgttaattta gaagacatac ctgaatatgt acataaaata gtttttggag tttctatata
     4621 caaagctaaa aaaagagacc aggattttgg tcaaatgaac aactctttta taagattaat
     4681 tgattcaaat tccaagcaag aattatttaa atataatctt caagaagact tttctggtaa
     4741 agtaacagtt ttggcaggag aaatttatcg tagaaatgaa gaatggaaat ttaatgcttt
     4801 aggaattggt caaaatgaag agcttcgtga attaattaat acatataagt aataattaca
     4861 ataaaaaatt tataatgagg agttggttta aatgggtata actttagcaa aaggacaaaa
     4921 agtaagttta acaaaaagta accctggtct aaaaaaagta atagtaggac taggatggga
     4981 tattaataaa tatgatggtg gctttgattt cgatttagat gcctctgcat ttttaactgg
     5041 aactgatgga aaagtaacga atgatggaga ctttatattc tataataatt taaaacacgc
     5101 ttctggagct gttgaatata tgggtgataa tagaacaggt gttggtgatg gtgatgatga
     5161 acaaataaat gtagacttat ctaaaatacc tcaaaatatt gctaaaatat catttagtgt
     5221 tactataaac gaagcaatta caagaagaca aaatttcgga caagttgaaa attcatatat
     5281 aagaatatat aatgaagaaa ctaatgagga acttattaaa tatgaattgg gtgaagattt
     5341 tagcatagaa acagcaatag ttgtggcaga actatataga cataatggag aatggaaatt
     5401 taatgcttta ggttctggat ttgaggatgg acttgcagga ttgtgtaaaa attttggcgt
     5461 taatataggc taatatatct aaataaaaat aagagtatat tgatatactc ttatttttat
     5521 ttagatatat tataaactgt ttacacttct actcttcttt ctcgcttgtt ttttcccata
     5581 ttctactgaa atatcaaaaa atgctgaaat attttctgta aaaaacttgc ttctatgata
     5641 tgcaatatca aatgtcctat gtaaattaat accttcaata tcacaagccc acaaaagatg
     5701 cttttttaaa taatcctcaa taagtaattc ggatataact gcaatgccaa aattatctac
     5761 aactgctctt aatatagctt cagagttata acatgaccat ctaatattca ttgggatttt
     5821 taattctttt aattgctttt ctaattgtgc tcttgtacca cttcctaatt cacgcataat
     5881 taatggttga tttgaaagtt ctgaaacttt tatagagtct cttttataaa actcatgctt
     5941 gtgtgaacat ataagtacaa gattatcatt aataatgctt tttgtaacaa tatctgggtg
     6001 cttaattttt ccttctacaa tcgcaatatc taattcacta ttaagtaatt tttttgcaat
     6061 cagtcttgta tcttctacaa atacttcagg ttctataaga ggatttacct tgtataattc
     6121 taacattatt ggacttatta cacatgtacc aacagttact gttgccccaa ctcttatacg
     6181 gatatttttt gcacaatgct tcatacaatc attcatttct tcatttacag ctaaaagatg
     6241 cctcccatat ccaagaagtt ttttccctgc ctcagttaaa tataattttt ttgaaattct
     6301 atcaaatagc ttaacaccat actctttttc tatttctgaa atagcttggc taactgatga
     6361 ctgggtaata tacatatttc ttgctacttc actcatttta ccacattcag caaccttaac
     6421 aaagatttca agactacgaa ttgtcatatt atcacaacct tagcaaacat tagtaaatgc
     6481 ttatgatttt attaatattt tagtatttta caaatatatt gtcaagaagt ataattttat
     6541 tatagagtta taaagtagat tggagtgtga tatattgtga aagtattaat tataggtgga
     6601 gttgctgctg gaacaaaaac tgctgcaaaa ataaaacgtg aaatgggtga taattgctct
     6661 gttacaatac taaataaagg tgatgatatt tcttatgcag gttgtggtct tccatattac
     6721 gttggtaaga taattgaaga taaatcatct ttaatcgtaa atacacctga aagtttttct
     6781 aaactaacag gagcagaagt tcgttgtgga gttgaagtta catctattga tagaaatgca
     6841 aaacaagtaa ctttgaaaac gctttcaaca ggagaaaaag aaaatctaag ctatgacaaa
     6901 cttgtaattg ctacaggggc agacccaata aaaccaccaa tacctggaat agatttacct
     6961 ggagtattct ttgtgcgtac tccaaatgat gctataaaac taagagattc tattgaaaac
     7021 ggtattaaga gagctgttgt tattggtgga ggtttcattg gtcttgaaat tgctgaaaat
     7081 ctttctgcaa tgggtatacg tgtaagtgtt gttgatatgg ctgaacatgt tatgcctgga
     7141 tttgatactg attttgctga atacgttgaa aatcatatgg ctgataaagg cataatgatt
     7201 tttacaggtg accaggttgt aggtatcgaa ggtgaaaata aagttgaaaa acttcgtact
     7261 aaaaatagag ctattaaaac agatttagtg gttatgtcag ttggaatccg tcctaacaca
     7321 ggttttctaa aagacacagg tttagaattt gcccctaata atacaattat tgcagatgaa
     7381 tatatgctta caaatgacaa agatatatac gtagttggag actgtgcttt tgtaaaaaat
     7441 tctttaacaa ataaacctac atggtcacct atggggtcat ctgcaaatca tgagggtaga
     7501 atttgcgcac aaaatatatc tggaaaatct aaaacttaca atggtgttct tggaacaaca
     7561 gtagtaaaat tacctgagct caatgcagga aaaacaggtc taagtaaaga aatagctgaa
     7621 aaagaaggtt ataatgtttg ctctgtaacc atagctactg atgataaagc tcattattat
     7681 ccaggtgcat ctaattttat tataagaatg gttgctgaaa aaaatactag aaaattatta
     7741 ggtgttcaag ttatgggacc tggagcggtt gataaaatag tagatatcgc tgctactgca
     7801 ataactttag gcgctgacct ttattcatta gaaagtatgg accttgcata tgcaccacct
     7861 ttttcaacag ctattcaccc ttttgtagtg gcagtaaatg ttttacaaaa taaacttaat
     7921 ggagaacttg atagtgtttt acttgacgaa attgaggatt ttgacagttg gactaaactt
     7981 gatgtttcaa aagctccttc tattcctagt ttacgttata ttcctgttgg tgaaattaat
     8041 ggagaaattg aaggccttgc taaggatgaa aaaattcttc ttatatgtgc taaaggtaaa
     8101 aatagctata tggcgcaaaa tcgcttaaga cgctttggct atacaaatac gaaagttctt
     8161 gaaggtggaa tactatttta tccaaatctt aaggtaatgg aggagtaaat atgagtaatg
     8221 ttacagcaga acaaaaaaag gaagtaaaag gtagaggttt tctaccaagc agggatggag
     8281 aacattttgc agcacgtata ataactgtaa atggtgtaat aaatgcatca caagcaaaaa
     8341 aaattgcaga agctgctgaa aaattcggaa acggtcagtt agcatttact actagactaa
     8401 cagtagaatt acctggtgta aaatttgaag atattgaagc tctaagtgaa tttattgcta
     8461 gcgaaaatct aataacaggt ggaactggat caagggttcg tcctgttgtg gcttgtaaag
     8521 gtactgtttg tgttcatggt ctagctgata cacaggcact tgctgctgaa atacacgaag
     8581 aattttacaa aaattggtat gatgtaaaac tacctcataa gttcaaaatt ggtgttggtg
     8641 gatgccctaa taactgtatt aaacctgacc taaacgatct tggaattgtt ggtcaacgtg
     8701 tacctgacta tgattcagag ctttgtgttg gttgtaaaaa atgtgctgtt gtagaggtct
     8761 gtcctgttaa agcagctaaa ttaacagata aaggtaaact tgaaatagat agtaatctat
     8821 gtaataactg tggtaagtgt atagaaagtt gtaattttga tagtattgaa gaaaaagaat
     8881 ctggttataa agtctatata ggagggaaat ggggaaaatc agttcgtcct ggaacacaga
     8941 ttgatagatt attttcaaaa gaggaactta tgactttaat tgaaaaagca attcttttat
     9001 ttagagaaca gggtaacaca ggtgaacgtt ttggaataac tatcgataga atcggtgctg
     9061 ataaatttat agaaatgctt ttatcagatg aagttttgga aagaaaagaa gagatactag
     9121 aggctctttt acaccttaca ggtggagctg tatgctaaca ataaatattt taattgtaca
     9181 gataacctat ggaaaaaatt ttagttaaga ataattaaaa ttctatatat gatatatgat
     9241 aaatgaggct atctcaaaac acagatacgc ctttttttca ttgttgaaat gataaagtat
     9301 atttgagttc atataaaaaa gagtgtctcg tgaattataa aattcatttt gaggcaccca
     9361 cattatttta taataattgc ttattaattt tattttaact atataaaaag tacaaaaagt
     9421 aagaatatcc aatattctaa aattaatttt gaattgtatt gattgaaaag tttactattc
     9481 cactatttcc atcttgattt actactagat accatgttcc agtattagga actctcattc
     9541 tacatggatt tttctttgca aatccaccat agtaatcata atttctttga tttttaaatt
     9601 tcacataatt tatatggtct aacaatatta cattggcttg tttctctaaa tttacctcta
     9661 caatatcccc tttttttaag ttcatcttac tataactata ttccattgaa ttactcctcc
     9721 agtatcgaat aatctttgtt atttactcat ctcaaatatg taaaaattca tttaaaatat
     9781 tacaaatact atataattag actattatcc agttttaacc ttatttatta caatccaaag
     9841 gttattaaaa atattattgg caacaacttc tccatcccat acaactcttt cttctttatt
     9901 ttctaataaa gcatagtacc cagagtcaga actttcatct ttatagactg taacataaaa
     9961 ctcttcatca ctatattcag gtatattttt aaagtcttca gatattttac atgttaatat
    10021 ataaccttct aattcttcat ctataaacaa agcaattc
//
LOCUS       DBGJFU010000134        10009 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.135, whole genome shotgun sequence.
ACCESSION   DBGJFU010000134 DBGJFU010000000
VERSION     DBGJFU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.135"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(140..1354)
                     /locus_tag="ACOIAS_003138"
     CDS             complement(140..1354)
                     /locus_tag="ACOIAS_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702828.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETMNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(1819..2754)
                     /locus_tag="ACOIAS_003139"
     CDS             complement(1819..2754)
                     /locus_tag="ACOIAS_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HGZ2702829.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            3042..3608
                     /locus_tag="ACOIAS_003140"
     CDS             3042..3608
                     /locus_tag="ACOIAS_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2702830.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            complement(3799..5835)
                     /locus_tag="ACOIAS_003141"
     CDS             complement(3799..5835)
                     /locus_tag="ACOIAS_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HGZ2702831.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(6009..6686)
                     /locus_tag="ACOIAS_003142"
     CDS             complement(6009..6686)
                     /locus_tag="ACOIAS_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGZ2702832.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            7029..7157
                     /locus_tag="ACOIAS_003143"
     CDS             7029..7157
                     /locus_tag="ACOIAS_003143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702833.1"
                     /translation="MSRIERRKKDRESKLAKVKTALSILPILINLIDKLIKLLKDL"
     gene            7403..7579
                     /locus_tag="ACOIAS_003144"
     CDS             7403..7579
                     /locus_tag="ACOIAS_003144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702834.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            7770..7898
                     /locus_tag="ACOIAS_003145"
     CDS             7770..7898
                     /locus_tag="ACOIAS_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702835.1"
                     /translation="MGRLERRKNKRENKFNKIKNAFSFTLVLINLVLAILRLIKEL"
     gene            8127..8312
                     /locus_tag="ACOIAS_003146"
     CDS             8127..8312
                     /locus_tag="ACOIAS_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HGZ2702836.1"
                     /translation="MILLEQRKLRVAFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(8353..>10009)
                     /locus_tag="ACOIAS_003147"
     CDS             complement(8353..>10009)
                     /locus_tag="ACOIAS_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2702837.1"
                     /translation="SNNVFLKNLSCSGNNLSKLNIKGLTSLEYLGCAKNKLQDLDVST
                     NIKLDSLFCGKNSLTKIDISKNKELKSLECSGQYIEKSIIKNGENIVVDIGIENSKLK
                     ELYPQNYNISTGFLTWKNISEFPNNVSYSLEYEYGPVNDVETMDVNISIDKLVKCRIR
                     YIDYDGSIINEKEISDNSSINEIQINPQRENYEFNGWRNNKDEKIYTSEQVSKLVFDD
                     STDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKLTGADRNETSVKISQKGWNKAD
                     NIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLK
                     SVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAV
                     SMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSV
                     TKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPV
                     VLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLVK"
BASE COUNT         3364 a         1574 c         1067 g         4004 t
ORIGIN      
        1 gataattcag atttctattt tgaaacctct atttttctaa ataactgttt aagaccgaca
       61 aaaaaggtga ttcaaatttt tattttgaaa cactttcttt taatttgatt aatctttaag
      121 tcctatatta aatttttact tattctttat ttttcaacat tttttccata tctaaaatct
      181 tttcaattac atccttaaaa attggttctt ctctaagctc attataacta tcatcattaa
      241 gtacaattaa caagtttgta tatatattaa aagtatataa atttttagac tctaccttat
      301 caaaaaatct catttgactt acatccaaac tttcatgtat aggatatttt tctaatattt
      361 cttttagact atataaggct ttttttgctt cttctttcat gttaaaaaat aaaaaaatta
      421 gagataattc catataatcc atatacatac taaaaaatgc acctttatct tcactgagca
      481 ttttttttat ttctaaagaa agatttgcat accccagcaa cttatttttt atatactcca
      541 tttctctctc aattgcattt ttattaaaat ttctattctc caattttttc tttaattcct
      601 tgtattgatt tttgtataca ttaattattc ccttacaatc catgcttata ttaaatacat
      661 tactaaacaa tttattttga agcaatattt ctgcttcttc aagtctattt tgctttaaat
      721 atatcatagc taaattaaca cttgtatccc ctattgactt atgtatcata tttaaataca
      781 actctgcctt ttcataatcc tccaacatac aatatgcgtt tgataatata ataagagctg
      841 tttcttttat ttggatatca tctgtatttc tcactatatc ttcaagaata ttcattgttt
      901 cttttactct gtcattaagt tcactcttac caaatttata tgaatacatc tgatacagcg
      961 aacatatcct tagcttcaat aaatagcttt taggatactt ctctatatac atattggaaa
     1021 gcttttttac agattctaag tcttgtctaa attcttcttt tggttcttct gttgataaat
     1081 caatttcaat tctagcaaag cctgattcta attctttata aattttcatt acttcttctt
     1141 cacttaaatc aatcttgtaa tttaaaagct tatcaataga cacattaaaa aagtctgcta
     1201 ataaaggcaa taattctata tctgggtaag aatttccact ctcccactta gatactgctg
     1261 gtgttgacac acctataaac tttgctaatt gttcttgagt taaatttcgt tcttttctaa
     1321 gacactgtat gactttacct attttaattt gcatatatac ctccctagta catctgttgt
     1381 aatttaagta tataaaaaaa atatttacta gtaaatcgag ggaaagttaa caaaatgtct
     1441 gtaatttaac cattggttat attttaatct aaaaccctat ctcattggct tgaaatataa
     1501 aatctattaa ttaagtttaa gtataaccta attaatagat tcacattaaa tagcatttaa
     1561 atatttagat tttctcttta ttacaactta ctatatttaa actttcaagg aattttaaat
     1621 aaaataaatt ccatatacaa tgataatcat actgagtgtt aataatttat ttataataac
     1681 acccacctat tcaataaatg gcattatagc aaaaaatcac ttctaggctc tctcttttct
     1741 actttgcata tacataatcc acttttcaaa tattatatat aaataaattt aaagcaaata
     1801 tatcattata tttttttatt acaatatttc tattttatta tttaaccaac aaattatatc
     1861 ctcaaaaact tcttctctat ttatttcatt caacaattca tgccttccat ctttgtaaag
     1921 tttgcaagtt acatctttta ctcctaattt tatatatcta tcccttagtc ttaagactcc
     1981 tttaccattt tttcctatgg ggtctttatc tccagatatt atgtaaatag gtatatcaag
     2041 tggcactttt tttaagtttt ctttatcctc aatttctttt aacccttgaa ctaaatcata
     2101 aaaaaagcca caactacaca ctactccaca aaatgggtca tttatatatt tttcaacttg
     2161 ttctttatct ctacttaacc agtcaaactt agtcttttca tttcttctta ttatttcgcc
     2221 accaaatatc aaattattta ttttattact tttagaacgt cttccatact ttttaatctc
     2281 acgattgatt atcagatgag caagatttag aatgattcct tgctttccat ttgaaccaca
     2341 tagtataaga ccagataaat tattactgta atccattata tatctttgac ttgcaaatga
     2401 acccatgctg tgtccaaata gataaattgg caaatcttca ttttctttct ttataatatt
     2461 tgttaaagta tacatgtcct ctacaagaca tctaaatcct tctttttcag ctaaatgtcc
     2521 aacattttct attatctttg cagtttttcc atgacctcta tggtcattga tatatactat
     2581 ataaccattt ttagtaagta ccttggcaaa agtctcatac ctttgtgcag tttcagccat
     2641 tccatgtgca atttggataa ctgcctttgg tttttttata ttttcatctt cccatttata
     2701 tgtatatata tctaaacctt cctctccttt gaaagtaaag tttgtacact tcataattat
     2761 ccctccagat tgattttatt cagtatatta atttatatat tatattacaa gtattttcaa
     2821 gtcaaaatct ttctaaaaaa tttaagaatt attaattcct catagaaatt atcattggta
     2881 catagaagtt tttacataaa attatacaat aatttaatta attaatagtt acataaaaaa
     2941 gtaatacttt aatatatttt gacaccatat tgacattaga attaattcat gatattctgt
     3001 tttcgacaaa acaacatcta aaaataaagg agatatattt catggattat tcaaatgaac
     3061 taaaagagct ctttttaatg aatcaaacat atgcaactct ttttaccctt acaaacaaaa
     3121 tacaaataga aggagataaa tattttggta tacttacttc aagacagtat atgactattt
     3181 tatcaattct tcatttacca gaggaagaaa caacattaaa taatattgca agaaaaatgg
     3241 gtacaagtaa acaaaatata aatagactag ttgctaatct tgaaaaaaat ggatatgttg
     3301 atgtaattcc aagtcctcat gataagagag caattaatgt taaagttaca gacttaggaa
     3361 aaaaggttat ggtaacatgt agtaggactg gaataaactt tatggcagat gtatttcacg
     3421 aatttaccaa agatgaatta gagaccttat ggagtttact aaaaaaaatg tatcgtttta
     3481 atggtgaaga acaagatggc tttgaagaag atgctaattt tatggaatat gaagaaatag
     3541 ataaaattaa atctgaagca cttgaagaat ttgcaaaaag aagaaatagg gtaaataaaa
     3601 aagattaata ctatcgcatc tataatacca cttttttata gtacaatatt ttatataact
     3661 ataatgtcat ttttattttt taacaaaaca atcattacat agattaggtg ttcttaggaa
     3721 ctctttttct gttttttata caaaaaaagg agatgattag acttttattt taaagcatct
     3781 cctttttcgc tattatgatt attctactcc taaaatcata tcttttaatt tttgtaaatc
     3841 attaattggt tgactacaac tattactttg acacacgtaa taactagtct tatcatcctt
     3901 caatatatag ttatttaaaa aacctataat agtgttttct tcattgtatt tctttgctaa
     3961 aaaagttgca tttggtataa aattttcact tatcaattct ttaaatgcaa ttaaatcaga
     4021 atcttcctta aaaatacata taatttcctt agtagaataa agttcaaaca tcaatgaaag
     4081 catataaaag ctgtagccag ttggagaact ctttacatta tccacataca gttttaattg
     4141 tttatatgac atttcttcaa gtctattatc tccagtaatt ttcgcaagtc ttataagatt
     4201 gtataattga acagaatttc ctgatggaat agctccatca tacaaatctt ttggtcttgc
     4261 tattaaattt tcactatctt taccatatat ataaaatcca cttttttcat aatcccaaaa
     4321 aaggttgata cagctttcat tcaaatttaa agccttttct aaatatttca tattaaatgt
     4381 tgactcataa agctctatat acgcccagat taaaaaagca taatcatcta aatatgctaa
     4441 atagtcggaa cttccatctc tatatctagc taataatcta cctgactcat ttacaagatt
     4501 attatttata aagttaagac acttatttga atactctaaa tatatgtcat tttttaaagt
     4561 actatatgcc tttgttaaag caactatcat caatgcattc catgatgtaa gtatcttgtc
     4621 atctttatgc aaagatgttc tttcttttct atattcaaat acctttttac taaggtcagc
     4681 tatcttttca ttatgtctct catattcttt attttttata agattaggaa tacttttgcc
     4741 ttcaaaattt ccactactag ttatatcaaa ataattatta aagaaaattc catcttcttc
     4801 acccaaaact tcaattatct caagaggatt aaatgtataa aatttacctt cttcaccttc
     4861 actatcagca tcctgtgctg aataaaaccc accttcttta tccttcatct ctctaactac
     4921 ataatctata gtttttatag caatttcttt atataactct ttctttgtta ttttatacgc
     4981 atctaaaaaa gctatagtaa gcatagcatt atcatatagc attttttcaa aatgaggagc
     5041 caaccacttt ttatcagtag agtatcttga aaacccaaat ccaatatgat caaacaagcc
     5101 tcctctatac attccatcta aagttttttc caccattttt aacacatctt tgtctttttc
     5161 tatactataa tatttcatta aaaacataag attctgagga cttggaaatt taggagcatt
     5221 accaaaacca ccatactttt catcataaat agccttaaat actctaactg atgaactcaa
     5281 catctctttt gataaatcac cctcagtatt ttttacacca aaatcatcct taagagcttc
     5341 tattatttca tctccagatt ttattaatat atccctgctt gtattccact tttcagatac
     5401 attctctaat aaatctataa ctccaggtct attatacctt gaatattttg ggaaatatgt
     5461 tcccgcaaaa aaaggttttt tatctggtgt cattattatg gtcattggcc atccaccact
     5521 acctgtcatg gcttggcaaa ctgtcatata cacgctatct acatcaggtc tttcctcttt
     5581 atctactttt atcgccacaa agtttctatt cattatttca gcaacttctt catcttcaaa
     5641 agattctttt tccataacat ggcaccaatg acatgttgaa tatcctacac ttaaaaatat
     5701 tggcttatcc tcttcttttg cttttttaaa agcctcatca ttccaactat accagtttat
     5761 aggattatat gcatgctgta aaagatatgg agatttttca tttattagat tattaggttt
     5821 tctattactg tccaaaatac cacctcattt gttattttat tatatgtttt gcaataaaca
     5881 aatagatata cacttataat aagtgtatac ccatttttaa ccatacatat ttaatttttc
     5941 atataattaa ttctcctagt aaataatttt tataactact aaaacaaaac aacataaaaa
     6001 atatcaaatt atttttttaa aggtttttca aaagaaggag gattaatatc tatcttagta
     6061 tcgtgatttg gccctggagt agacaaatcg aaatataatt tagaatcttc tgtaactcca
     6121 aaatctctat ttgaacctgt atctttaact ttatttaaag catctctaaa tagagcatta
     6181 tgtgcttctt ctctattaag taagaaatct attgtttctt ttacatattt atcatctatt
     6241 tgtctataca agtactcata aactacctta gctctttgtt cagaagctat atttgaaagt
     6301 aagtcagcta ctaaatcccc tgtaactgta acgtaattag cagtccaagg agctcctgat
     6361 gagtttatta agactggtga tagaccagtt aatacatgtg tttctatttc gcctgtatta
     6421 actgaattat aatcaacatc atgtccattt aataaattta tagtttgtgc taccatttcc
     6481 atatggctaa gctcttctgc tgctatatca agaaataaat cttttatttg agggtctttt
     6541 attctaaaac tttgggacaa gtattgcata gctgctttta actctccatt tgcaccaccc
     6601 aactgttctt gcattaaaac agcatattga ggatttggtc tttctacttt tacttctttt
     6661 aataatgctt tatcgtgttt aaacatatga atacctccgt aaattattat tcaaaattat
     6721 tatttactta agttgtaata ttattctagg ttatttgttt attgttgtta ttttttacaa
     6781 gtgttggtat ttcaatgttt tattaaggtc agttttgttg tttatgtttt attatgttta
     6841 atttaaattt attctattat ttattttcgg tggcaaaagg gtggcaagtt tggttttgcc
     6901 acccttattt ttatttgtaa aaaaatatag tttgtcaata taactttatt gaaaaatatg
     6961 gaaataaata tcgcttttgc cggttcggca tattataata taaatataga aagggggtga
     7021 aaaggaaaat gagtagaata gaaagaagaa aaaaagatag agaaagcaaa ctcgccaaag
     7081 ttaaaactgc tctctctatc ttacccattt taataaattt aattgataaa ctaattaaat
     7141 tattaaaaga tttatagttt tgtaagctag gaagaattta atcttcttcc tagtgacttc
     7201 tttctaaata ttataacaca ttttctataa aatatgagtg ataaagttta taaattctgt
     7261 cttattatag ttgctataac tatagtaata gacataatac ttttattttt acattttaat
     7321 atcaacaata taataggatt aatagtaagc attttattgt taatatttgt tacattacaa
     7381 tataaaaaag gaggaaaata gtttggagca aaaagaatct aaaatagttt acagtaaagg
     7441 tggttcaggt acttattcgg ctaaaatttc tttaccttta agccaacttg aaaaaatggg
     7501 atttacaagg gaaaaacgta aggctgtagt tatttttaag gaagatgaga ttataataaa
     7561 aaaatctgat gaggaataac tctaaaataa gtgggtgtat aatgtaaaca tccacttatt
     7621 tttattatat tttgtatttt atattaatat attttatagc tttattgaaa aatatggaat
     7681 atttattgac tttcacgtca cgtgatgata taatataaat atagaaagga ggtaagaaaa
     7741 taaaaaactt agaagggagg acaaagaaag tggggagatt agaaagaaga aaaaataaaa
     7801 gagaaaacaa atttaataaa attaaaaatg ctttctcttt cacattagtt ttaattaatc
     7861 tagtactcgc aatactaaga ttgattaaag aattataagt ttcaatccta ggaggaaaat
     7921 accttccttc taggtagctt ctttctaata ttataacaca ttttctaatt taatatgaat
     7981 aataaatttt atagaatttg tatgattatt cttggtatca ctatagtttt aaatattatt
     8041 aatataataa taagttttac ttttaaagct cttattgctt tggtatttac tttattattg
     8101 ctggcattta ttaatagtag aaaggaatga tattattgga acaaagaaaa cttagagttg
     8161 cattcaataa aagtggcaaa ggctctttta cacccagatt gatacttcca atgtcatgga
     8221 ttaaagagat gaatatctct cctgatgaaa gggatgttct tgttactttt gaggatggaa
     8281 aaataattat tgaaaaaact gaaaatgaat aaaaaaggag gtagtaacta tttccagtta
     8341 ctacctttca atctatttaa ctaagttttc tacttcatta aatgcttttt catttccatt
     8401 tccaccaatt tgagttatat ttttaaactt tttattcttg attaactctt tttggctatc
     8461 atctaatcca tttccaacta atactactgg agattcagtt ttacctgcaa gcactcctac
     8521 tgataaggca tctactaaat cgtcttgttt attcattcca ttcttagcta catataagtc
     8581 attaagtgta tctttgctat agaaatgact tattactttt gagttagttt ctgttctatc
     8641 agaacctgct attttagtta cagatggtaa tgtgttattc acttctttat tattaaataa
     8701 agattctcca cctattacat atgattttga tatgttttta ttagctatta agtcttttat
     8761 atctttcata tcatcatttt gattagtaag aagaattggc atttcctctt ttgctgatat
     8821 agctcccata cttactgcat cagctagtcc cttttctcca tttaccacta ctacttttga
     8881 caggttagaa tttttgtcta attcttttgc aagttttatt gatgtttcat atctatcatt
     8941 tccagatatt ctaattacat ttaatccttt tttctttaaa tcagacacta ctttttcatc
     9001 aacagacttt aatccaccca ctatgtacac attttttgct tctaatctat ttatttcttt
     9061 ttctgttaat ttatttaagt tattattttt agtaagtaat attggtgcat ctttagactt
     9121 agcaaatggt gttgctgata aagcatccga tatgctagag tcatttatta acactatatt
     9181 atcagcttta ttccatcctt tttgacttat tttcactgat gtttcatttc tgtctgcacc
     9241 tgttaatttt tccttagatg gtgctatatt aaaatcttct ttacttgaag ttccaccaga
     9301 agaaccactt gatcctgttg attttctttt ataagttgct ataaaatcag tactatcatc
     9361 aaaaactaat ttacttacct gctctgatgt atatattttt tcatctttat tgtttctcca
     9421 accattaaac tcatagtttt ctctttgagg attaatttga atttcattta tactagaatt
     9481 atctgatatt tctttttcat tgattatact tccatcataa tctatatatc tgattctaca
     9541 tttaactaat ttatctattg atatatttac atccatagtc tctacatcat ttactggacc
     9601 atattcatat tccaaactat aacttacgtt atttggaaat tcagaaatat ttttccatgt
     9661 tagaaaacct gttgagatat tataattttg tggatataat tcctttaatt tcgaattttc
     9721 aattcctata tctacaacta tattttcacc attttttata atactttttt ctatatattg
     9781 accactacat tccaatgatt ttaattcttt atttttactt atatctattt ttgttaaact
     9841 atttttccca caaaataaag aatctaattt tatatttgta gatacatcta aatcttgtaa
     9901 tttattctta gcacatccca aatactcaag acttgtaagt ccttttatat ttaatttact
     9961 taggttatta cctgaacaac ttaaattttt caaaaaaaca ttattactt
//
LOCUS       DBGJFU010000135         9842 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.136, whole genome shotgun sequence.
ACCESSION   DBGJFU010000135 DBGJFU010000000
VERSION     DBGJFU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.136"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            224..1510
                     /locus_tag="ACOIAS_003148"
     CDS             224..1510
                     /locus_tag="ACOIAS_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HGZ2702838.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1622..2095
                     /gene="grdA"
                     /locus_tag="ACOIAS_003149"
     CDS             1622..2095
                     /gene="grdA"
                     /locus_tag="ACOIAS_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110943085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1754..1756,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HGZ2702839.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2127..3437
                     /gene="grdB"
                     /locus_tag="ACOIAS_003150"
     CDS             2127..3437
                     /gene="grdB"
                     /locus_tag="ACOIAS_003150"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3174..3176,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HGZ2702840.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3693..5225
                     /gene="grdC"
                     /locus_tag="ACOIAS_003151"
     CDS             3693..5225
                     /gene="grdC"
                     /locus_tag="ACOIAS_003151"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HGZ2702841.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5236..6381
                     /gene="grdD"
                     /locus_tag="ACOIAS_003152"
     CDS             5236..6381
                     /gene="grdD"
                     /locus_tag="ACOIAS_003152"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HGZ2702842.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6691..7770
                     /locus_tag="ACOIAS_003153"
     CDS             6691..7770
                     /locus_tag="ACOIAS_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGZ2702843.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7883..8482
                     /locus_tag="ACOIAS_003154"
     CDS             7883..8482
                     /locus_tag="ACOIAS_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702844.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8669..9541)
                     /locus_tag="ACOIAS_003155"
     CDS             complement(8669..9541)
                     /locus_tag="ACOIAS_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2702845.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
BASE COUNT         3798 a         1128 c         1864 g         3052 t
ORIGIN      
        1 ctgaagataa gaagaaaaag gtttttatgt ccaactttgt taaaaaaata attttaaata
       61 aaataatttt tttacaataa atatttttta gtaataaaat aaagatttaa ggcataaaaa
      121 cacaataata tatctttaaa aaaataagat aaattgtaaa atgatataat tttgagaaaa
      181 actcaaatgc atatacacaa attaaataac aggaggtgca agtatgcgtc ttgaattagg
      241 gaaaatcttt ataaaagata ttcaatttgg tgatgtaaca gaagtaaaag atggagtatt
      301 attcatcaat aagcaagaga tgctacaaga aattggtgga gatgagcata ttaaatctat
      361 agatatagaa ttagctcgtc caggtgaaag cgtaagaata acacctgtaa aagatgttat
      421 tgagccaaga gtaaaagttg aaggtaatgg gggaatcttc cctggtatca tgtctaaagt
      481 tgacactgta ggagaaggaa aaactcatgc tttaaaaggt gtagctgttg taactactgg
      541 aaaaatagta ggtttccaag aaggtataat agacatgact ggagaaggag ctaagtacac
      601 tccattttct aaattaaata atgtagtagt aatagctgaa cctattgatg gtttaaaaca
      661 atacgctcat gaaaaagctg ttagaatgat aggattcaaa gcagcaatgt acttaggaga
      721 agtagcaaga aatcttactc cagatgaagt ttcagtatat gaaactaaac ctcttcttga
      781 atcaataaaa gaatatccag agcttccaaa agtaggatat gtttacatgc ttcagactca
      841 aggtttatta catgatactt atgtatatgg agtagatgca aaacaaatag taccaacttt
      901 attatatcca acagaattaa tggacggagc aattgtaagt ggtaactgtg tttctgcatg
      961 tgacaaaaac ccaagttatg ttcatataaa caatggggtt gttgaagact tatatgcaag
     1021 acatggtaaa gatataaact ttgtaggtgt aattataact aatgaaaatg tttatctagc
     1081 agataaagaa agatcttcaa actggacttc taaattatgt aaatatttag gattagatgc
     1141 agttatagtt tcacaagaag gttttggaaa tcctgatact gaccttataa tgaactgtaa
     1201 gaagatagaa atgcaaggtg ttaaaacagt tatagttaca gatgaatatg ctggacgtga
     1261 tggaggttct caatcacttg cagatgcaga tgtaagagct gatgctgttg taacaggtgg
     1321 aaatgccaac caagttgtag ttcttcctaa gttagataaa gttattggac atttagaagt
     1381 agtagatgta atagctggtg gttctgatgg aagtttaaga gctgatggaa ctattgaagt
     1441 tgaaatccaa gctataacag gtgcaactaa tgaaacagga tttggtcatt taactgctaa
     1501 aggatattaa gaagtatttt attaaacgat tgagtaatgt taataaaaca tattatagat
     1561 aattataata agtgttatag atatataaaa atatattaga aatataataa ggaggattat
     1621 tatgagttta cttagtaata aaaaggttct tataataggt gaccgtgatg gtataccagg
     1681 acctgcgata gaagaatgtg taaaaacagt agaaggagca gaggttgttt tctcatctac
     1741 agaatgcttt gtctgaacag ctgctggggc tatggactta gaaaatcaaa acagagttaa
     1801 agatgctgct gataaattcg gagctgaaaa tgttgtgatt ttactaggtg ctgctgaagc
     1861 cgaagctgca ggtcttgcag ccgaaacagt aactgctgga gatccaactt tcgctggacc
     1921 acttgctgga gttgccttag gattaagtgt ttaccacgtt gttgaggaac caataaaatc
     1981 attatttgat gaaagtgtat atgaagacca aataagtatg atggaaatgg ttttagaagt
     2041 tgaagaaata gaagaagaaa tgtctggtat aagagaagaa ttttgtaaat tttaatgcct
     2101 aaaagcaaaa ggggtgaaac taagagatgg gaaaactaaa agcagtacat tatattaatc
     2161 agttctttgc tggtataggt ggagaagaaa aagctgatac taagccacat gtggcagaaa
     2221 ctttacctcc aataagttta caacttgata aattattagg tgaagatatt gaaatagttg
     2281 gaacagtagt ttgtggagat agctatttca atgaaaatat agatagtgca agtgaagaag
     2341 ttttatcaat ggtaaaaggt tttgaaccac aacttttcat agctggtcct gcatttaatg
     2401 ctggtagata tggggttgct gctggaacaa taactaaagt tgtaaaagat gctttgaata
     2461 taccagcttt aacaggtatg tatgttgaaa atccaggagc agatatgttc aaaaaggatg
     2521 tttatgtagt agaaacttct gattctgcag caggtatgag aaaggcactt cctaagatag
     2581 ctaaacttgc agtaaaactt gctaatggag aagaaattgg aactcctaaa gatgaaggat
     2641 acatagctag aggaataaga gttaactact tccatgaaga cagaggttct aagagagctg
     2701 ttgacatgtt agttaagaaa ataaaaggtg aaccatttga aactgaatac ccaatgccaa
     2761 actttgatag agttgaccca agtaaagctg taaaagattt atcaaaatgt aaaatagctt
     2821 tagttacttc tggtggtata gttccaaaag gaaatccaga tagaatagag tcttcttcag
     2881 cttctaaata tggaacttat tctatagcag gagttatgga tttaacagaa gagacttatg
     2941 aaactgcaca tggtggatat gacccagtgt atgcaaattt agatgcagat agagtattac
     3001 ctgttgatgt tcttagagat ttagaaaaag aaggtgtaat tggaaaatta catgagactt
     3061 tctatacaac tgtaggtaat ggtacatctg ttgcaaactc taaaaaatat gcttctgaaa
     3121 ttggagcagc attagtggct gatggtgtag atgcagtaat attaacttct acatgaggta
     3181 cttgtactcg ttgcggtgca acgatggtaa aagaaataga aaaaactgga cttccagtag
     3241 ttcatatgtg tacagtagta cctatttcat taacagtagg ggctaataga atagttccaa
     3301 ctatagcaat acctcatcca cttggaaatc cagcgcttga ccctacagaa gaaaaagccc
     3361 taagaagagg ccttgtagaa aaagcattaa atgctttaac tacagaagta gatggtcaaa
     3421 cagtatttga aaaataaaaa taacacacac atatattata tagataaaaa tataaacaca
     3481 cactaaaaat aaaaaataac tatacacaaa aaaataatat agatttcatg aatggataaa
     3541 ttggagttat tcattcatga aatcttaaaa aatgccaaaa tacataaaca ataatagaat
     3601 caaatataca ataaaataca atgattagag cttttaataa gtttaataag attaaaaaat
     3661 aagtgttcat aaaatttatg gaggtgcaca aaatgactta tccagtttta aaaggggcag
     3721 gctatgtact tattcatact ccagatatga tagtgcaaaa tggaagtact tgtacagttg
     3781 aaagagcaac aaatccagat tcagaatttt taaaagaagt aagtaatcat ataagaagtt
     3841 atgaagatgt agttaactac atgccaaatc aagtttatat aggaaataga agaccagaag
     3901 aattaagaga tttaccaatg ccatggtgtg aacaaaagat tgagggaact agaaatggta
     3961 agtttggaga aataatgcct caagatgagt tcatagcttt aatgcaaata agtgatgcat
     4021 ttgatttagt aaaattatca caagaattta ttgatgaagt aaagccaaaa atcgaaaata
     4081 actaccctga aattgcacca tttgtaggta aattaaaggg tgatgatata gaagaaggta
     4141 aagaattagt agcaacacac atagctgaag gattatacca tgatggaaaa tttgttggtt
     4201 atgtaaaaag agcacatgat gtagatgtta acttaaatgc ccatacaatg tttgaaaatc
     4261 tagtagtaaa agcatctgga gttttatctg ctattcaaat gctaagacat agcaaaatag
     4321 accctgctga aatagattat gttattgagt gttcagaaga agcttgtgga gatataaacc
     4381 aaagaggtgg aggaaacttt gctaaatcaa tagctgagat agcaggactt caaaatgcaa
     4441 ctggttctga cacaagagga ttttgtgcag caccaactca tgcattaatt caagcagcag
     4501 ctttagtaaa agctggcata cataaaaatg taatggtagt agcaggtgga gcaagtgcta
     4561 aacttggaat gaatgctaaa gaccatgtta aaaaaggtct tcctgtactt gaagatgttg
     4621 ttggaggatt tgcagtactt gtatctgaaa atgatggtgt aaatccagtt ataagaactg
     4681 atttaacagg taagcatact gttggaactg gttcttcacc acaagcagtt atgacagcac
     4741 ttataacttc aggtcttgat agagctaatt taaaaataac agatgttgat gtgtattcag
     4801 ttgaaatgca aaacccagat ataactaagc cagcaggagc aggagatgtt ccagaagcaa
     4861 actacaaaat gataggggca cttgctgtta aacgtggaga cttagaaaag aaagaattga
     4921 aagattttgt ttctaacaaa ggattaccag gatgggcacc aactcaagga catataccat
     4981 caggagctcc ttatatagga tttttaatag atgatttaac tacaggaaat agaaatagag
     5041 ctatgatagt tggaaaaggt agtttattct taggaagaat gactaactta tttgatggag
     5101 tatcttttat agctgaaaga aatacaggtg ttactgaaga gacttcagga atatctaagg
     5161 atgaaattaa gaagataata gctgaatcta tgaaaaaact agcattagat atgttagaag
     5221 aataggggtg aggatatgtc aaaaaaagta atcgcagatg tattcttaga agtagctaat
     5281 gcaatagaaa gcggcgaatt tggcaaaaaa gtaaaaattg gtgttactac acttggaagt
     5341 gaacatggtg tagaaaacat ggttaatggt gcacaattag caaaatctaa tttatttgat
     5401 attgtgctta ttggaccaaa agtggaaact gaccttgaag tagttgaagt taatgatgaa
     5461 aaagaaatgc atgcaaaaat ggaagaatta ttagattcag gatatattga tgcttgtgtt
     5521 actatgcatt ataattttcc aataggtgta tctacagtag gtagagtaat aactccagca
     5581 aaaggaaaag aaatgatact tgcaacaaca acaggaacaa gtgctactaa tagaatagag
     5641 gcaatggtaa gaaatgccat ttatggtatt gctacagcta aatcaatggg taacaaatgc
     5701 ccaaaagttg gaatacttaa tgttgatgga gcaagacaag ttgagaagtg cttaaaagaa
     5761 ttaaaagata atggatatga catggagttt gcggattcaa ttagagcaga tggtggatgt
     5821 gtaatgagag gtaatgattt acttgtaggt gcacctgatg ttatggttac tgatacatta
     5881 tctggaaata tatttatgaa agtattttct tcatatacta caggtggaga ttatgaagct
     5941 caaggctttg gatacggacc tggagttgga gaggactatg atagaaaggt acttatagta
     6001 tcaagagcat caggttctcc ggtagtagca aatgctttaa aatacgctta tgatgttgta
     6061 aagggagata taagtaatgt tgcaagaaat gaatttgcaa aggttaaaaa agcaaaattt
     6121 gatgacatta tatcctctct tactaaaaaa gaagttaaag ctgaaaaagt agaagttaaa
     6181 atgccagata aagaaattgt tactcgtcaa atagctggtg ttgatataat ggatttagaa
     6241 gatgcagtat ctgagttatg gaaaaacgga atctatgctg aaagtggtat gggttgtaca
     6301 ggtccaatag ttttagtaaa tgaagctaaa ggagacttag cagttgaaac tttagttaaa
     6361 gctggatata ctgcaaaata agtacatttt ttatatgtaa ataagaacat atagttataa
     6421 ttttattgat tataattata tgttcttttt attttatatc ttgtcaatat ttatatatta
     6481 acacaatcca tttatttgat tctctagcta atttattata tttattcaat taaaattaag
     6541 ctttctaact aaataaaaac taatgaagta ttaaaatgaa attgataaaa tatgaaaaaa
     6601 ttttaggaat aaagtatctg ttagataaaa ataagattaa ttagtgatat aatataatta
     6661 agataaaatt tattatttgg aggtaaaatc atgagcagag taaaaaatgt agtagaattg
     6721 ttggaaacta aaggtgttga tgcactatat ctaacaaaga aaactaatgt aaactatata
     6781 agtggttttc ctgatgaaga agcatatgca gtgatttgca aagatggaaa ctttttagtt
     6841 actgatagta gatatatgga gcttgcagaa aaggtttgca aagattttga aattataaat
     6901 tggcataatt ttgatagaag tgtggcaaag gcagttaaaa gtgtatgtga taaagtgggt
     6961 ataaagaaac ttggatttga aagaactaat atagtgtttg ataaatatga agaacttaaa
     7021 aatctgattg agaaggataa tggagaatta atacctacag aaaatattgt agagactttg
     7081 agatatgtaa aagacaaaga tgaaatcaaa aatacaagaa aagcttgtga aatagcggat
     7141 aaggcattag aagaattgat tccacatata aaagctggag ttagcgaaat tgaactggct
     7201 acaaggttag agtactttat gaaaatgaac ggagcacaaa atatagggtt tgaaactata
     7261 ctgatatcag gagcaaagac ttcattgctt catggtaagc ctagtgataa gattatagaa
     7321 aaaggtgatt ttgtacttat agactatggt gctatgtata atggctatat atctgataca
     7381 actagaactt ttatagttgg aggagcttct gaaaagcaat tagaaatata taatttagtt
     7441 aaggaagctc aaaatgtagg ggttgagaac atgaaagctg gtgtacatgc gacaattcca
     7501 gatgctgaga taagaaaagt tgtcaaaaaa tatgaagact attactatca aggtatagga
     7561 catggagtcg gtagagatgt tcatgaagaa cctttcattg gtaattatgg tgataaaatc
     7621 atagaagagg gatgtataat aacaatggaa ccaggtattt atttccctgg atggggtgga
     7681 gttagaatag aagatactgt tttgattact aaaaatggtc cagaaagact tactaaattc
     7741 cctaaagatt tgatgatttt agacaaataa tttaatttat atatattttt ttacataaaa
     7801 tataacataa tctcactatt aagctctatt tacatattat agtaaagagg gcttaatttt
     7861 attgtgagag gtgatagtat atatgtataa aaaacataaa tttataaaaa atataaaaaa
     7921 agcctggtat aaaatcaaat taattgatag atgccttata attgtaatgg caatattaat
     7981 gtttcaatct atatataact tatttgttaa tgaagtaaat tcacaagata ctacaactat
     8041 tgatgtagta gtaagaacca cttcagcagc tatatttggg tattttttaa gtgctaattt
     8101 tataaagaga cctttaagga agtatacaaa tactgatgta tcttcaaatc tttttataga
     8161 taataattta tccccaaaag gtcaagagtc taatcaaaat aatataatga atgtcaaaaa
     8221 taccattgga tttacttcag aagctgataa ttatgaaaag aaaataccta ttaataataa
     8281 tgaaggtttt gaagaaggag aaactagtga attgcaaatt attatagcta ctgtaatttg
     8341 catagtagca ttaacagtac tttttgttgt aagaaatttt acaactacaa catcagcttc
     8401 tttaggtact atttcacaga tgagagattt tgtctcagga tgcgtgggtt ttttgctagg
     8461 ttgtccaagt aaacctgatt aaaaaagtat tatcaataaa aggtatttta aaaagtatca
     8521 gtataataat atttagattt aaatgtatac aattataatt ctgtaacatt ctatattgta
     8581 tttttatact gatactctaa actatactat ttattaaaat attttaacat tgtatttaaa
     8641 caaaatatta tatctgtata atataaattt agtttatatg aatagatgcc atatactcaa
     8701 taaaaagttt catactatga gatatgtatt gatttcttct ccaaagaacc ccaatatttg
     8761 cagtaattgc acgttctaat ggtattgaac agagattttc tgtattttga aatatttctt
     8821 tatacaatat tgttgaagcc aagtcatttt ctagtaaata acgtatggta gaaagttgat
     8881 ttgagtgtaa aattatattt ggcttagctt tttcaagtct ttttgtaata atcttatagt
     8941 gataagaacc ttcagataag attacaaaag gtacattttt taacatataa ttcgatataa
     9001 taggatgttt tgccaattca tttttgggat ttgtaactaa gtataattct gttttataga
     9061 ttgtctttga atctatatca tcataaatat cactgtcaat tataccaata caaaaatcaa
     9121 gtgtagattc ttcaatcatt ttagctccaa caaatgttgg aacttcaaaa attttaagct
     9181 ctatatctgg ataaattaca ttaaacctag gaagtatttt tgaaagcaaa aacgtaccta
     9241 aaactgtagg aatacctatt ttgagagaag cttttctctt ttttcctaaa tcaagtgcgt
     9301 cacattgaaa atcctcaaat tgttttataa aatgttttga cttgtctaaa aaaatttttc
     9361 cttcaggcgt taactctatt ttattaccta ttctgtaaaa aagttcaaag ccaagttctt
     9421 tttctaattc tttaattgct aatgttatgg atggttgaga aatatgaata ctttccgcag
     9481 ccttagtcat gtttttatat ttacagacag tttgaaaata gtataaatag tttatattca
     9541 tttggtatta gttcctttca tttacatatt agtgacaagt atataattaa caatagcata
     9601 agttttgtat attgttgtaa aaaaatagaa tagttttaaa ctatcatagc ataagttttt
     9661 gatattttca atctattaag aatagtgcta ttattaaatt aacaaaagag aatagctggt
     9721 atggtttaga gtaaatcaat ctatattaca gttatatcaa cgaatatcaa aaaattatct
     9781 attaaaaaat aaaaattatt tttacaatta aattgttaat taaatcataa agaaaaaagt
     9841 ga
//
LOCUS       DBGJFU010000136         9806 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.137, whole genome shotgun sequence.
ACCESSION   DBGJFU010000136 DBGJFU010000000
VERSION     DBGJFU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.137"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            220..684
                     /locus_tag="ACOIAS_003156"
     CDS             220..684
                     /locus_tag="ACOIAS_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HGZ2702846.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            1321..2337
                     /gene="gap"
                     /locus_tag="ACOIAS_003157"
     CDS             1321..2337
                     /gene="gap"
                     /locus_tag="ACOIAS_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGZ2702847.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            2732..3901
                     /locus_tag="ACOIAS_003158"
     CDS             2732..3901
                     /locus_tag="ACOIAS_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702848.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            4281..8534
                     /locus_tag="ACOIAS_003159"
     CDS             4281..8534
                     /locus_tag="ACOIAS_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HGZ2702849.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            8683..9177
                     /locus_tag="ACOIAS_003160"
     CDS             8683..9177
                     /locus_tag="ACOIAS_003160"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGZ2702850.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            9293..9670
                     /locus_tag="ACOIAS_003161"
     CDS             9293..9670
                     /locus_tag="ACOIAS_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HGZ2702851.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         3968 a         1006 c         1754 g         3078 t
ORIGIN      
        1 ttttaaataa gtgatttgac tcttaaattt tcataatatg ttataaacca atttaagata
       61 ctacaataat taaaattcca aaattattta acatatttgc aattaaaatg agaaaatgta
      121 aaggctgaat gattgaaaaa tttgatgtaa aatagtagct tagtttaata ctatagtttg
      181 tagggttaaa aattagaaat tgtaagggag ataaattcta tgaaaaaatt agttattatt
      241 ttaattagta tcattagtat ggttagcttg acagcttgta aaaaaagcaa tgaaagtaaa
      301 tataataatt taaacaacaa agaatccgga caaacaacca ttgaaatgga cttagataaa
      361 aattatgata cttcagaccc atttgttaat gcacgtttgt tttgtgtgtc taatgatata
      421 gatgttttag acacagagat ttcttttaaa atggatggag atagtggtat tgtggaaata
      481 aaggacaata aaacagatga aactttatgg agtaatacat ggcatggaag cgttgataat
      541 gatacattca caatttctct ttctaatata caaaaagaga aagagtatgc tatatgcttt
      601 actggaatta aaataaatca tgcagttgta aaggttactt ttgaaagcaa tttagttaag
      661 gagaaagaaa aaccctcaaa ataaattcca gcttttctat aacttgatat tcagtacata
      721 aaagcagttg atatttatga ttgactgctt tttcttatca aaaaggatag gttgtgcatt
      781 tttacttgaa atagtttatg cacatcaaaa gttggtgagc atgatattgg aaatggtaag
      841 catgaaatct tgataataca aggaaataaa tttaagtttt caattaagta tatttccata
      901 atttagttaa caataagcta attattattt atattttgat atataaataa taattgtagc
      961 aaattttaaa aaatcatata ttttttgaac taaataaaaa tttattttta ttattttaaa
     1021 ttagaggaga ttcttgaaga tatattattt ttaaaaataa gtatagttta aaaattcaaa
     1081 aaaaagatgg caaaatatgt cccaagggac attgagtaaa ataaatgatt tcagcttatt
     1141 tacactaaaa agtacatact aaatgagggg taaaataaag aaaaatgata tactaattgt
     1201 cttttgataa taaaaaaaca tggaatttga ttatttaatc aagaaaaagt attgaacaaa
     1261 tgatttattt agtgtacaat aatcatgtaa aaacatagta atggagggag acatttcata
     1321 atgaaaatta aggtaggaat gaacggattt ggaagaatag gaagagctgt attaagaata
     1381 gctcaagaag aattaggaga taatatagaa attgtagcca taaatgcaag agcaacaaca
     1441 gaatctttgg cacatttatt tacatatgat tcatgctatg gaacttttag aggtgaagta
     1501 gaggcaaagg atgaagacac tctagtagta aactctaaag agataaaaat attaagatat
     1561 aatgacccag aagaattacc ttggaaagaa ttgggggttg atatagtaat agaatcaaca
     1621 ggattattta cacaaagaga aaaggcagaa aaacatatta aagcaggagc aaaaaaagta
     1681 attataacag ctccaggaaa aaatgaagat ataactatag ttatcggagt taatgagaaa
     1741 gaatatgata atgaaaagca caatataatt tcaaatgcat cttgtactac aaattgtcta
     1801 gctccttttg ctaaagtatt ggacgaaaaa tttggtatag taaaagggtt aatgactaca
     1861 gtacattcat atactaacga tcaaagaata ttagataaaa gccataaaga tttgagaaga
     1921 gcaagagcag cggctgaatc tataatacct accactacag gagcagcaaa agctgtgtca
     1981 agagtactac cacaattaga aggaaaatta aatggttttt cacttagagt accaacacca
     2041 acagtttcac ttgttgattt agtgtgcgaa cttaaagaaa acgttacatc tgagcaagtt
     2101 aactctgtgt taaaagatgc tgctgaagga gaattaaaag gtgttttagg atattgtgac
     2161 aagccacttg tttctataga ttataaagga gattcaagat catctattat agacgctcta
     2221 tccacaatgg ttatagaaga caatatggtt aaggtagttt cttggtatga taatgaatgg
     2281 ggatattctt caagaactgt tgacttagta aaatatgtag ctgaaaaatt aaaataatat
     2341 atatttctta aagcaaattt taaaaaaatt ttttaaaaaa attatataaa actaaaaaaa
     2401 aagattatga aaaatattga aggaaaaatt ttttcataat cttttttctt tcatgcaaaa
     2461 agatacaagt tacaacttat atttgcatat taggtcgtta gagaagtata tatatattta
     2521 gtaaggaatc atacttttta taaaatgaaa gataagatgt cgaaaacttg cataaatctg
     2581 aaaaattttt attattaaaa tactgatttc cattttaaaa atgataaaaa aatgacaaat
     2641 aaagttgcaa atagtaatac ttataagtat aattaaaaaa gagaaaaggt tttgttaaac
     2701 tttataacaa aaaaagaagg gatgattcaa aatggagaag ttaacagaca ggagacaggt
     2761 taaggcagta agcatggaaa ccttcatttt cctaatatta ctagtagttg gttttgggta
     2821 tgttggtagt attatgggag caggaatgat gttcaaagta ataatgagta cagcacatgc
     2881 tttactattg gagacagttt tcttaattat ggctatggca gttcttgcag gcgcattaag
     2941 cgcactactt tcagagtttg gagtaatagc gttaattaat aaaattttcg ctgtatttat
     3001 gaagccttta tatggactac ctggtgctag tatagcagga gctataacaa cttatttatc
     3061 agataatcca gctattatac cttttgctaa agataaaaca ttcacacagt attttaaaca
     3121 ataccaagtt ccagcattat gtaacttagg aacagccttt ggtatgggac ttatcttaac
     3181 aacatttatg atttctcaag gtacagaata tgtacttcca gcattaattg gtaatttagg
     3241 tgcaattata ggtagtgtca taagtgttag aataatgttg acatttacta agaagtttta
     3301 taaatacaat ccggaagaag ataaagcaac aggaacatta gagaaaaagg atgaatttag
     3361 agaaatcaga gaaggaaatg tatttcaaag agctttagat gcaattcttg aaggtggtaa
     3421 aatgggggta gatatgggaa tggctataat tcctggagtc ctagtagtat gtacattggt
     3481 gatgttatta acttttggac catctacaga tcctgttaca ggtcaagaag tttatacagg
     3541 agctgcatat gaaggaataa aattacttcc agttattgga gataaacttg gatttatatt
     3601 agaaccatta tttggattta catcaccaga ggcaatagca ttccctataa cttcattggg
     3661 agctgttggc gcagcaatgt ctctagttcc agagtttata aagtcaggag ctataactcc
     3721 taatgatata gcagtattta cagctatggg tatgtgttgg agtggatatt taagtacaca
     3781 tattggtatg atggatgcat taaatgctag acagttggca ggtaaagcta ttctttctca
     3841 tacaataggt ggtttatgtg caggagcagc tgctcacttt atatttacac tcgtaggata
     3901 aatgtaatca gctaagataa aaaataatat acaaaaaatg gttgttgata tgtaataaga
     3961 atttacatat caacaaccat ttttttctga ctaaagataa tttataatac ataaacatta
     4021 agtggatatt agagttaagt tatatttatg taaaaagtaa caaaaatctg tagaaattta
     4081 tgacaaaatt tagtacacta actatatgtt cttatttaaa atttaaggtt agaattagga
     4141 attacctagt agagtaagct ttgattaata aaagaatcat aaaataaaaa tattagtcat
     4201 aaagattata aataacttac ttaaaataaa gccatagaac ttacaaaaat taaaaaagca
     4261 tataaatatg gaggggtaca atggatgaat tatttcatat aggaattgat gtaggttcta
     4321 caacggtgaa ggttgtggta cttaataata gtaataatat agttcataaa gaatatagaa
     4381 gacattattc tgatgtaaaa aaatcagtta aagaagtact aaatggtata tatgaaaaat
     4441 tgggagatat aaatactacc attataataa ctggttcagg tggaattgga atatcaaaaa
     4501 aattaggtgt aaaatttgtt caggaagtta tatctagtac aaaatcaata gagtattttc
     4561 atcctgaaac tgatgttgtc atagaacttg ggggtgaaga tgcaaagata acatatctaa
     4621 gtggaggaat agaccaaaga atgaatggaa tatgtgcagg tggcactgga gcattcatag
     4681 accagatggc atcactttta aagacagatg ccagtggatt aaatgaactt gctaaagggt
     4741 ataatgttat atatcccata gcttcaagat gtggtgtttt tgctaaaaca gatattcaac
     4801 ctcttataaa tgatggagcc aatcagactg atatagcaat gagtattttc aatgcagttg
     4861 tcgtacaaac tgtgagtgtc ctttcttgtg gaagaaaaat agaaggaaat gttgcgtttt
     4921 tgggtggtcc attgtacttt ttatctgaac ttagagaagc atttaaaaga gtattagatt
     4981 taaaagatga aaacataatc ttcccagaaa atgcacaact ttatattgct attggagcag
     5041 gattattatc agtagatgaa gataatatac aactaaaatc tctaatagaa aaattggata
     5101 gtataaagaa tattgaagat ggagaagtaa atttattaga acctttattt aaagataaaa
     5161 atgagtatga agaattttca agaagacatg aaaaagaaaa aataaattat gtggacataa
     5221 attcaataaa aagcaattgt tatttaggaa ttgatgcagg ctcaactaca actaaagcag
     5281 cacttattga tgaggatgga agacttgtat attcttatta taatagcaat gaaggaaatc
     5341 cattaaaaac aaccattaaa gttataaatg aagtatatga tatattacca aaaaatatta
     5401 aaatattgag ttcaactgta actggatatg gagaaggtct tattaaaaaa gctcttaaaa
     5461 tcgataatgg agagatagag actatagcac attacaaagc tgctaaattt ttcaacaaag
     5521 atgtagattt tatacttgat attggtggtc aagatatgaa gtgcctaaaa ataaaagatg
     5581 gagtaattga taatataata ttaaatgagg cttgttcatc aggttgtggc tcatttttag
     5641 agacatttgc aagttcttta tctatgacaa tagaagaatt tgcgcatgaa ggtatttatt
     5701 ctcaaaatcc tgtagattta ggttcaagat gtactgtatt tatgaattct agagtaaaac
     5761 aagctcaaaa agaaggtgca agtgttggag atatatcagc agggctatct tattcagtaa
     5821 taaaaaatgc tttatttaaa gtaataaaga ttagagattt aaatgagatt ggtaataata
     5881 ttgttgtaca aggtggaacc ttttataatg atttggtgct tagaagcttt gaaaaactaa
     5941 ttggaaaaaa tgtaattaga cctaatattt caggaattat gggagctttt ggttcagcac
     6001 ttatagctaa agaaaaatat gaaagtggat acaaaacatc cttgttatct aaggaagagt
     6061 tgaataatat caagctagaa gcaagtgtaa ctagatgtaa aggttgttca aatcattgtc
     6121 ttcttacaat aaataaattt tctgataatg aaatttttat ttcaggtaat agatgtgaga
     6181 aaggtgaagc tatttatggt gataaaaaaa taggattaga aaagaaaaaa ccaataaatt
     6241 tatttaagta caaatacaat agaattttta gatacaaacc attggaaaaa gaagaagcaa
     6301 aaaatgggga aatagggata ccaagagtat taaatatgta tgaagattat ccattttggt
     6361 ttactttttt caatgctatg ggctttagag ttgtactttc agataggtca tcaaaacagt
     6421 tatatgaaac tggtataact agtattgcat ctgaaacagt ttgttatcca ggaaaattag
     6481 ttcatggaca tatagaaaat ttaattgcaa aaggtataaa aaatatattt tatccaagtg
     6541 taacaaatga aaataaagag gattcaaatg cagataatta ttataattgt cctgttgtaa
     6601 tatcatattc tgaagtaata aaaaacaatg tagagaatat aagaaataag aacataaatt
     6661 atataaatcc atttataagt ctaaatgaca aggaaaaatt gaagaaaaga ctctatgatg
     6721 aattatcaag acatttttct gatataaaaa taactaaaga agaagttaat catgcagttg
     6781 ataaagctac tgatgaacag aatttattta aaatggagat acaagcagca ggtgaaagag
     6841 cacttaaaga aataaaagaa agaaatatga aggggattgt gttatgtgga agaccatatc
     6901 atatagaccc agaaataaat catggaatgc cagagcttat aaattcatta gatatggcag
     6961 tgcttactga agactctata tgtcatttag ccaatgtaca aagaccactt agagttgtag
     7021 accaatgggt atatcattca agattgtata aagcagctag ttttgtaaga gataaaaact
     7081 atttggaact tgttcaactt aattcatttg gctgtggatt agatgcagta actacagacc
     7141 aggttcaaga aatacttaat gaaaaatcga agatatatac tattataaag attgacgaag
     7201 gcaacaattt aggagcagct aagataagga tgaggtcctt aaaagcagct atgtttgaga
     7261 gagaaagcaa aaatatagat gtaaaaaata taaaggtaaa caggatacag tatgctaaaa
     7321 ataatcagat aacgaacaaa catactatat tagcaccaca aatgtctcca atacatttcc
     7381 aattccttga aaaagctgtt aacttaagtg gttataatat agaaattcta aaagatactg
     7441 atagtagtgt aattgaagaa gggttaagat atgtaaataa tgatgcatgt tatccagcta
     7501 taatagttgt aggtcagcta ataacagcat taaaatctgg caaatatgat ttaaataaca
     7561 cttctgttac tataactcag actggtggag gttgtagagc tacaaattat attggattct
     7621 taagaaaggc tatatacgat gctggattta aagatattcc tgtcattgca cttagtgtaa
     7681 atggaataga agatagtggt ataatggata atatatcact aaaattaatc aatagactct
     7741 ttatgtctgt tgtttatggt gatttgctga tgaaagtact atatagagta agaccatatg
     7801 aaaaagttac tggaagtgca aatgctctat atgaaaaatg ggttgatata tgcaaatctt
     7861 ctcttgttaa agcaaaaatt tctgtattta ctagaaatat aaaagatata gtaaaagaat
     7921 ttgataattt agagatacta gatatcaaaa aaccaaaagt aggtttggtt ggagaaatat
     7981 tggtgaaatt ccatccaatt gcaaataata atttagtaga tatattagag cgagaaggtg
     8041 ctgaagcagt cgtgccagac cttacaaatt tcttcttaag ttgtgccttt aatactatat
     8101 acaaacacac tcatttagaa ggaagtagaa agagtagaat gataggagaa gcatttatat
     8161 acataactgg aatatatcaa agagtatata aaaaagcact tgataagagt gagagatttt
     8221 atgctccagc caatataaaa aatgttgcta aaagtacaga accagtagtg tcattaggta
     8281 atcaaactgg agaaggttgg cttttaacag gtgagatggt tgaattatta aatgaaggtg
     8341 tagaaaacat tatttgtatg caaccttttg gatgtttacc taatcacata attggtaagg
     8401 gttctataaa agaattaaag aggttgtata aaaatgcaaa tataatacct attgattatg
     8461 acccttctgc aagtgaggta aatcaactta acaggataaa acttatgctt tctaaggctt
     8521 ttaaaaatat ataagattta tattttgata tgttaaaagt taatgaattt aaaaatatac
     8581 tctatattaa gtatctataa aaaagagtta tcctattttt tagggtaact ctttattagt
     8641 taaaaaatta tatgtatact gatattaaag gggggaagta atatgagttt agtatatgat
     8701 gtttataaat caaaattagg aaatttgtac atattaagtg aagatggttt tatagttagt
     8761 atatacatag gagatgaaaa atttaataat ttaaaaaatg aatgtattaa aaaaggaatt
     8821 gatataaaga gaagtaagga agatttaaag gaagcaataa gccagataga tgattacttt
     8881 aatggaaaaa gaaaagtctt taatttaaat attcgtattg aagggactga atttcaaaaa
     8941 tcagtttata gggaaatgtt aaaaatacct tatggagaaa cattgtgtta ttcagatata
     9001 gcaagaaata taaacaatcc aaaatcagta agagcaatag gacaagcaag taaaagtaat
     9061 aaaattccaa taataatacc ttgtcataga gtagttggca aaaaagatat tggtggatat
     9121 atggggaatc attctgattt gaaagagata ctattaaatt tagaaagaca gagttaactt
     9181 agaaagaaat gtttttattg ttaatatttg atataataat agtaaaaaga agtatttttt
     9241 acaaaaacaa tgaagaattt attttaattt ataaaaatga gggggaattt taatgtgtga
     9301 taaagttata gcatcaaaat atcatgagaa gggatttaat tgtgcagaat cagttataaa
     9361 agcatataat gaggaattta atacagatat accagtttgt ttaggaagtg gactgggttc
     9421 agggtgtggg gttgctagtt tatgtggtgc agtaaatgca tcaaatataa taattggata
     9481 tgtaaaaggc agaaaccacg aagatgaaag tactaaagct aaagtatatg ctaaagattt
     9541 aactacaaca gtaagaaaag agtatggttc agagttatgt atagacctaa aaaaagattt
     9601 agttgcttgt agagaaataa tggatttcgc atatgatagt ctaaaggaaa ctttaaaaaa
     9661 agaattataa gaaaataagc tatcaagtat aaattatttg atagcttatt tttatagtcg
     9721 atactaaata aatcaaatat attttagaat tattatttgt aaataaacag caaataattt
     9781 taggattttt atttaatata gttttt
//
LOCUS       DBGJFU010000137         9693 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.138, whole genome shotgun sequence.
ACCESSION   DBGJFU010000137 DBGJFU010000000
VERSION     DBGJFU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.138"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(220..1005)
                     /locus_tag="ACOIAS_003162"
     CDS             complement(220..1005)
                     /locus_tag="ACOIAS_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGZ2702852.1"
                     /translation="MNFENDFITPEFRKKYIGLVLDNAQNINKVYTTTFPDKEIIKQV
                     IDKDEKDILIFSHHAMGYIASDEGFPFHDIPLSYMEEMKNRRISFYVLHSPLDNYSDY
                     STSVSFAKAMGLEIVKPFCKYDDKIEVGVICKTSLKSIEEIKELIKKSVGHDVKLYDY
                     GDSVLKDGLVAIAAGGGSYPFVAREVAELGINLYITGFTKPLKHFEPVLEFHQIAKDN
                     LINVIGATHYSTEKFACMGMVDYFKNLGLESEFLQGKYYLEDL"
     gene            complement(1393..1863)
                     /locus_tag="ACOIAS_003163"
     CDS             complement(1393..1863)
                     /locus_tag="ACOIAS_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2702853.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(2597..2986)
                     /locus_tag="ACOIAS_003164"
     CDS             complement(2597..2986)
                     /locus_tag="ACOIAS_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2702854.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(4785..6191)
                     /locus_tag="ACOIAS_003165"
     CDS             complement(4785..6191)
                     /locus_tag="ACOIAS_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2702855.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVEFDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(6236..6943)
                     /locus_tag="ACOIAS_003166"
     CDS             complement(6236..6943)
                     /locus_tag="ACOIAS_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702856.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            7105..8028
                     /locus_tag="ACOIAS_003167"
     CDS             7105..8028
                     /locus_tag="ACOIAS_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702857.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQKLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            8028..8789
                     /locus_tag="ACOIAS_003168"
     CDS             8028..8789
                     /locus_tag="ACOIAS_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702858.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMFMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            8801..9538
                     /locus_tag="ACOIAS_003169"
     CDS             8801..9538
                     /locus_tag="ACOIAS_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702859.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         3299 a         1558 c          984 g         3852 t
ORIGIN      
        1 aataataatc taaaatacat caaaaaataa actaaaaaat atcgaaaaat aatttaaaag
       61 acatcaaaac taaatcttct aatcagcaat taaaagtcta ttttcttagt ttaaaaaata
      121 aagatagatt ttttcaaaac actggataaa taggtaaaaa aataaacagt tagtaaaata
      181 aatttttact aactgtttat ttttattgtt taatatgtcc tataaatcct ctaagtaata
      241 ctttccttgc aaaaattctg attctaaacc tagattttta aaataatcga ccatgcccat
      301 acacgcaaat ttttctgtag aataatgagt agcacctata acattaatta aattatcttt
      361 tgctatttga tggaactcta atactggttc aaaatgtttt aaaggcttag taaacccagt
      421 aatatataga tttataccaa gttcagcaac ttctcttgct acaaatggat aacttcctcc
      481 ccctgctgca atagcaacta aaccatcttt taatacagaa tctccataat catacaattt
      541 cacatcatga ccaacagatt ttttaattaa ttccttaatc tcttctattg attttagtga
      601 tgttttacat ataactccta cttcaatttt atcgtcatat ttacaaaatg gtttgactat
      661 ttctaaaccc atagcttttg caaaactaac agaagttgaa tagtcactat agttgtctag
      721 tggagaatgt aatacataaa aagaaattct acgatttttc atctcttcca tataagatag
      781 tggtatgtcg tgaaatggaa aaccttcatc tgatgctatg tatcccattg catgatgtga
      841 aaaaattaaa atatcttttt catctttatc tataacttgc ttaattattt ctttatcagg
      901 aaaagtagtt gtgtatactt tattaatatt ttgagcatta tctaatacta atcctatgta
      961 tttttttcta aattctggag taataaaatc attttcaaaa ttcataaaac tccaatcatc
     1021 attaatattt ttaatattaa attctaaatc taatttttta taaagttcaa ttgccttaat
     1081 aataaccact cctttataat aatattgaat tactacatat taatatagaa atttccgata
     1141 ataaacttta tacgtttaag tattcctact tcaaattaaa aatattataa atattcaaat
     1201 ttattaatat attttaatat tatatttatt ttttctttat taatattccc cttattttta
     1261 tttatttttt ctttattaat attttcttta ttttaattta atttatgaca tttgtcaaat
     1321 actagtccta tatattatct tgaattagtt ctattacaat taatttctac taaataatta
     1381 taatttaaac aatcattcct tactattttc acactgcttt acaaatttaa ttggatgttg
     1441 aattatttta gaagtttctt ctacagagta tccctcagat aaatatcttc tagctataac
     1501 ttccatagac tctcctatct caataatatg cttatccggg tcataaatac gcactactct
     1561 ttgttgccat tcatgtttta gtggcttatg gacaaactct acatctttat aataattgag
     1621 tttttcaata aattcgtcaa aatcatcaac ctcaaaataa agttccatat tatttgattt
     1681 ttgtataatt gtatctggat taatccctgt aagccatgcg aagtcttctt gtatagcaaa
     1741 tcctccttta aatgttacat ttttacctaa gtccaattca atttcttgct caaataattc
     1801 tttgtaaaat ttttttgata cttcaatatc cttcactgct aataatgaaa cctgatattt
     1861 cataaatttt cctcctaatt ttttatttct attttttcag tatcttctat taacattttt
     1921 ttctctatct cttcagtacc ttttattaat cctttatttt tactttttta gtatcttcta
     1981 ttaacacttt tgtttctacc tcttcagtat cttatatcaa tcttttattt tttcaatatc
     2041 ttctattaac acttttatct ctatctattc agtactttct atcaatcttt tgtttttatt
     2101 ttttcaatat cttctattaa tacttttgtt tctacctctt cagtatctta tatcaatctt
     2161 ttattttttc aatatcttct gttaacactt ttatctctat ctctttaata tcttatatta
     2221 atcctttatt tttatttttt caatatcatc attaattcct atttatttta tcatcttaaa
     2281 tacgaaaaca ctgtgtcata tttgcaaaat atatattatc atctatatta aagcttcaca
     2341 atttatattt aaaaattctc tttaattaca ctacctctat ttaaaaagct accttaatta
     2401 tacttataag taaaggtagc tttttaaatt taattaaata gaatatccac tattaatttt
     2461 tatttaattt atttagattc acattatagt atcataatcc acttataatc atttgtcata
     2521 ttacttaagt agactaatat attacataaa tacacttaca tacactttat attgatgcta
     2581 taagaaatac gtttgcttat acgtctttct cctcttcttt tagatttttt atccactctt
     2641 caataagtaa gatatcatct ttgctcaaat taatattttc atgtaatgac aacaataaac
     2701 tttttataga agaatcatga atatttctaa aaaaatctct tgtctcaact cctatgtatt
     2761 ccttttcttt aattaaaact tcataatgcg tatatttatc tatcttttga gaatttagaa
     2821 atctttttac tactaatcta gataaaagag tcaaagttgt cgtttgtttc cattgatatt
     2881 tttgctccat tcctaaaaca atatctctag atgttacttt ggaatctgat ttccatataa
     2941 atttcattat ctttaattct gattgtggca acttttttat agtcatgata aaaccttgct
     3001 actcagaata tagacaagca atatccataa taaaattttt cgattaaata catcttatac
     3061 ggatttatat aagctcagac ttataagata tttttcaatc ttaatcctaa atttagctat
     3121 ttgtacacgg taaatatata taaataatgt caaaattctc ttgtattata ttgcttaatt
     3181 ttatgaaata ttttgtactc agctatctat tttggatact tgtccagtag ctctcctttc
     3241 ttttaaactc tttgatttta aatatttgta tttcagtaaa tcctacttcc taaatttttt
     3301 atttactaca acttattact gaacttccta cttcctaaac tttttattta ctacaactta
     3361 ttactgaact tctcacttac taaatttctt acttactata aattataact aaacttctca
     3421 cttactaaat ttcttactta ctataaatta ctactaaact tcctactttc taaatttctt
     3481 acttgctata aattattact aaacttccta ctttctaaat ttcttacttg ctataaatta
     3541 ttactaaact tcctactccc taaatttctt acttgataca aattattact aaacttccta
     3601 ctccctaaat ttcttacttg ctataaatta ttactaaact tcctactccc taaatttctt
     3661 acttgctata aattattact aaacttccta ctttctaaat ttcttacttg ctacaaatta
     3721 ttactaaact tcctacttcc taaatttctt acttgctata aattattact actaaacttc
     3781 ccacttccta agtttcttac ttgctacaaa ttattactaa acttcctact tcctaaattt
     3841 cttacttact actacttata actaaacttc ccacttccta aatttcttac ttactactac
     3901 ttataactaa acttcccact tcctaaattt cttacttact accacttata actaaacttc
     3961 ccacttccta aatttcttgc ttactaccac ttataactaa actttttact tcttaaattt
     4021 cttgcttact accacttata actaaacttt ttacttctta aatttcttac ttcttaagaa
     4081 taatgttaat tttaagatta tagtttttgc cttgtttaat tgaaacattc tatataatta
     4141 tatccttcta ttttagagtt tttttattta tatattacaa agtttaaatt ttttcattaa
     4201 ttatattata aatttcccat atatttcaac ataaatacat atatatatat gttatttcta
     4261 aaaatactac acaaaaactc tactttagta gaactcaatg gcgcaaaata cttcaattta
     4321 aaacatttat cgtactttaa ataaataact ataataatat acttacaata gttacataga
     4381 agctattata actacacaga acagcgtata attaaaactt ttttaggatg tcattatatt
     4441 ttttcttaaa attaaaaaac actataccat aattctattt tagtagaatt tttttgtcaa
     4501 taataaaatt aaatattaac taaaaaataa aaaaaatgga attatctaaa gaatttaata
     4561 accttacaat aattccaaaa tattatttct atgaaaattt tgataccatg agtaactcag
     4621 ccataagcgc ataaagcaca agttactctt tactttaata aaacttgaca agaaaagaat
     4681 ttatccttat tctactctaa tagagttaat ttgtcaataa agaataaaat tattttcaaa
     4741 atattttcat tcttcattct tatctgttat tattttataa aatatcactt aattagaggt
     4801 aattttagta ttgtttttag tccctgacta tcaatgctcg taatatctaa tttacctcta
     4861 tgtgcattaa taatgccaga tactatcaaa atacctagac catgattttg acctttggca
     4921 tatgcctcac ttagtccatt ttggctcttt aataaactat taattctttc ttgactcata
     4981 ccttttccat catcttcaac aattatatag caaaaatcta aatcagactt agaggtaacc
     5041 cttatattac atccttgttg gttatgagtt atactatttt gtattaaatt actaatagcc
     5101 ctttttaata attttttatc tgcatctata ataatatctt catttagtat atcaaattca
     5161 acgtaaaagt cattatccaa attattattt ataaactcag aaataagctc tctcaaaaac
     5221 gcagaaactc taatcttatc acaattcaat gcttgcatat tatattcaag tttagacact
     5281 aaatttaggt catttactaa ttctctgatt ttaatccctt gattgcatat aatagacact
     5341 tgctcttgtt ctctatttga aaggctactg ctttcttcaa gttctccagc atatccaatt
     5401 atcattgaaa gtggagttct tatatcatga gatattcctg caatccagtt agacctagct
     5461 tcctctttct tcctaagttt ttcatctttc atttgcaact ctttagatac agtattaata
     5521 gattttgaaa tatcacttag tattcctttt tcttgtaaaa gtactggttc tccttttgac
     5581 ataagcttta tgccagtaat aattggtttt atagatttca ccatttttaa acctatgaat
     5641 agagctatta aaaaagtaac tattgcatta accaaaaaca tacctattat taacatagga
     5701 aattgttcta tttccttaac cgtataagta aggttaaact tagtgtactt gtcttttgga
     5761 taacctgtta ctaatattcc atttggatgt tcccatacat acacaggata gtcctttaaa
     5821 aagaaccttg aaaactttgc tacatctgtc aaagtataat gacttggaat ttcttttggt
     5881 aaatcaaatc cccatattct atctccattg ttatcaatta acattgccca aactttattt
     5941 ttatgtagta cttccttact agaatcatct aaaataaact ttccatttct taaaatcaaa
     6001 ttatcttcaa tcataatcaa gtttgatttt atttctctgc tatcacttgt atctaaatta
     6061 tcagagtatt tagatacaaa tagcatatat gaaaacagaa gaaaacctgc tatatttaat
     6121 acaataaaaa gtaatgaaat caagatactt gaacctataa atcgcctcaa tatgtacttt
     6181 acactatcca aaattaaatc actcctcttg atttctttta ttctaatatc taaaattaat
     6241 taatcatcaa cttatatcca agacctctta ctgtaataag atgtttaggt gtagatggag
     6301 ttttttctat cttctctcta acccttctaa catgaaccat aagggtattt tcatatccat
     6361 acatatcatc tccccaaatg gcttgacata gtgaatcatt agtaacaatt ctccctttat
     6421 tttcatacaa ctttaaaagc aacgaatgct ctttagctgt taaagaaatc tcttgcccat
     6481 ctttctcaac acatgcacta tttaaattca caacaacaga atctcccagt ttaaatactg
     6541 gcaactcctc ttcttctata ggcacatata cacgatttaa tatacccaca agccttaaag
     6601 taagttcctt tggtaaaaat ggctttacta tataatcgtc agcccctaag cccaaaccaa
     6661 tcaatctatc ttcatcttca cctcttgcag agagaaaaat cactggtatt ttagatgtct
     6721 ctctaatttt tctcataata gaaaaaccat ctccatctgg caatacaaca tctaaaattg
     6781 cacaatctgg ctttacattt ctaaataccc taacagcttc ctcataactt gatgctaaaa
     6841 atactctact aaacccctct tttctcagga tttcatctat catttttaat aattccttgt
     6901 ggtcatcaac cacaagtatt tttttatctt ttattgactc cataatcatc accctccttt
     6961 agtataccat atacccattt tcacaacttt gcctaaatag acttttaagg ttattgtaag
     7021 gtaaatttca gctaattgaa atgtaaccaa gttattatac tgatataagt taaaagaatc
     7081 aataagcaca gattaaaaaa ggggatgaaa catatggaaa tgataattag aacacaaaga
     7141 ctttcaaagg aatacaataa aatatttaga gtaaaggata tagacctaag agttccacaa
     7201 ggagctgtat atggtttttt gggtcctaat ggagctggaa aatctaccac tcttaaaatg
     7261 cttcttggac ttgctaaacc cacacaagga aatattaata ttttaggtaa agagttaaat
     7321 gaaaaaaata gaatatcaat tttaaaagat ataggttctt tgatagaatc tccatcatac
     7381 tatggtcatc ttacagggct tgaaaacatg accataatgc aaaaactctt agatttacct
     7441 aaaaaaaatg taaatgaagc attaaaaatt gtaaggttag aaaatcaaaa aaataaaaaa
     7501 gttagtcaat attctttagg tatgaagcaa cgtcttggta ttgctatggc aataatgaag
     7561 tttcccaaat tattaatact tgatgaacct acaaatggac tcgacccagc agggattgag
     7621 gaaattagag aattaataaa atctctacca gaaaaatacg ggatgactgt tctaatctct
     7681 agccatcttt taagtgaaat tgaccaaatt gccacatcaa taggaataat aaatcatggc
     7741 gaactggttt ttcaagatag cataaaagaa cttcataata gaggtcaaag tcagattgct
     7801 atcaaaacta agaatgttgt atctgcacaa aaactattaa atactaaagg atatatgtca
     7861 cttatacaag atgggtactt gactattgaa aatttaaaag actcagatgt ggctaaaata
     7921 aatggtatgc ttgtccattc aaatatagat gtatttagaa tagaagaaca taaaaaaagc
     7981 ttggaagata tattcttaga acttactggt aaggcggtga ctttataatg aaataccttg
     8041 gtctagaatt ttataaactt aaaagaaaga agattattct gatgaccttt ttatttgtag
     8101 ctgttgaaat aatgtggtgt atcataaatt caaatagagc tattacacgt aatccagata
     8161 tgttaggtgg atttgaatat tcctatatgt ttatgagttt tgcaagttta aatggtctat
     8221 tctttcctat cttaatatca ataattacat cacgtataag cgatatagaa cataaaggag
     8281 atacatggaa gcttttaaaa tcttctgtga catctttaaa tagtatttat ttatctaaat
     8341 ttttatgttc tgctattttg gtatctatac ctgtattgat gcaagtgtta tctattgtgt
     8401 tgtttggaca ttttagaggg gttattgagt caatttctgt atctttactt ttaaaataca
     8461 cattaggaac aattcttgta agtattgcta tcattgcact acacctatgg attgcaactg
     8521 taattccaaa tcaaatgatt gcaattacat ttggcatgat tggtagtttt ataggtttga
     8581 catcaggttt gttttatcaa ggtattcgta gattgtttat atggtcttat tacctagagt
     8641 tatcaccttt aagttatgct tatgataatg ttttaggttc atctgtctat agaattgata
     8701 tgaatttttc tattacgatt ttagttttct taattggaat gctaatttat tatattggaa
     8761 gaaatcattt attaaaaaaa gaagtatagg agggatttaa atgttaagga agtcagtttt
     8821 gggagaaatt ctaaagttaa aaaattcttt tatatggtat gtattattag ctttgccact
     8881 tataagtgtg ttgattggtt ctggtaattt ttatctaaat caaggcatac tcaaaaaaga
     8941 atggtacagc ttatggactc aagttagtct tttttatggg gaatttttct ttcctatctt
     9001 aatcgcaatc ttttgtgcat atatttgtag gcttgaacat atgaatcata attggaataa
     9061 tgtaatgaca ctgccagtta atttaaaaaa tatcttttta tctaaactta cagtagtttc
     9121 aatacttaca gctattacac aagttttctt tgttatcttt tatatagttg caggaaatat
     9181 gtttggtttt tctggtagtg tacctaaaga attgactggt tggattgcta aaggttggat
     9241 tatttctatt tcaatagctt ctatacaatt atacatgtct ataaagatta gaagttttgc
     9301 aactcctatt ggtattagtc tttgcctttc tctatttggt atgggattaa tagtagcaaa
     9361 acctgctgtt ggtatatttt tcccttattc tatgttaggt agtgctatgg gggttataaa
     9421 gcaatctgct ttaaatgtta atgacactat agttacaata tgtgttgcat ttattactac
     9481 tatagtattt tctgaaattg ccaatagaaa ttttagaaag aatgatgtta tttcttaatg
     9541 gtaaatgcta gatattttgg ggtttgaaat ttatattctt tttagttgaa atctatatcc
     9601 attttattta aaaatatata tcttttattt gaattttata tgttttatct actcgctata
     9661 taatagatat actcctaaaa ttaaaaaata tat
//
LOCUS       DBGJFU010000138         9639 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.139, whole genome shotgun sequence.
ACCESSION   DBGJFU010000138 DBGJFU010000000
VERSION     DBGJFU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.139"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            119..790
                     /locus_tag="ACOIAS_003170"
     CDS             119..790
                     /locus_tag="ACOIAS_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2702860.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            792..1700
                     /locus_tag="ACOIAS_003171"
     CDS             792..1700
                     /locus_tag="ACOIAS_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2702861.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1777..2463
                     /locus_tag="ACOIAS_003172"
     CDS             1777..2463
                     /locus_tag="ACOIAS_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702862.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            2460..5015
                     /locus_tag="ACOIAS_003173"
     CDS             2460..5015
                     /locus_tag="ACOIAS_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2702863.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(5209..6408)
                     /locus_tag="ACOIAS_003174"
     CDS             complement(5209..6408)
                     /locus_tag="ACOIAS_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HGZ2702864.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6527..7414
                     /locus_tag="ACOIAS_003175"
     CDS             6527..7414
                     /locus_tag="ACOIAS_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGZ2702865.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            7604..8161
                     /locus_tag="ACOIAS_003176"
     CDS             7604..8161
                     /locus_tag="ACOIAS_003176"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2702866.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(8400..9260)
                     /locus_tag="ACOIAS_003177"
     CDS             complement(8400..9260)
                     /locus_tag="ACOIAS_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGZ2702867.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         3753 a         1164 c         1412 g         3310 t
ORIGIN      
        1 tattttgaaa acaaatttaa atacaatctt tttagtgaaa taaataaaat taaaatattt
       61 ttagtatgat aatgcaagaa aataattatt tatctaaaaa atctaaagga gagtaaaatt
      121 gaacttatta attattgaag atgatataaa cttaaatgaa ggtctttttt atgcatttga
      181 gaatgatgga tttaatgtat ttaaggctta tacaaaacaa gaaggattaa atatatttaa
      241 tagtaaaaat atagatttta taatcttaga ctgcaatctt ccagatggag atggatttga
      301 tgtatgtcaa ataataaggg aaaagtcaga tataccaatt ataatgctta ctgcaagaga
      361 ctcagaaatt gatgaagtaa aaggattaga aatagggctt gatgattata ttacaaaacc
      421 attttcttta tctgtattaa aggcaagagt caaagtagca ataagaaaaa aatcgaataa
      481 taaagtaata tattccaatg gaataaaatt ggaccaaaag ttactaaaag tatataaaaa
      541 taaagaatgt ttggaactta gctctgtaga atataagctt ctaagttatc taattgaaaa
      601 taaaggtcaa atattattaa aggaacaaat tttacaccat atttgggaca gcgaagaaaa
      661 ttatgttgat gataatattg tatctgtaaa tataagaaga cttagagtaa aagtagagga
      721 tgacccatct aatccaaaat atataaaaac agcttatgga atgggttatt tatggaatga
      781 ggtagaataa tatgtttata ttagccttta taatatgtac agttgttata tttatagtat
      841 ctactaaaat aaatcgaaaa agatatgatg aattaaattt gatgttaaat caaatattgg
      901 aaggtaaaga agttacttat ccagatacaa aggatacaag agcgtcaaaa atttctcacc
      961 aagtaaaaaa aattaaggat atgatagaaa tagaggtaga acagtcaaag tcagaaaaag
     1021 aagcgattaa aggtttaata tcaaatatgt cacatcaatt aaaaactcca ctttcaaata
     1081 taaccattta ttgtgaactt ctggagaata taaatatttc cacattacag aagaaggagt
     1141 ttttacaaaa gatgaaaaat gaaactttta agattgactg gctgttacaa tccttattta
     1201 aaatgactaa attagaggat ggagttatag aatttgaagt agaagaacta ctaattaaag
     1261 atactcttat tcaaagtatc agtactatct ttaataaagc agaagctaaa aatataagga
     1321 taaatctcga accattttca gacataaaat tagttcataa taaaaagtgg accatagaag
     1381 caattgtaaa tgttttagaa aatgcaataa aatactcacc atcagatagc acgattacta
     1441 tttctgtaat aaaaatggaa ctatatacga agattactat taaagatgag ggaataggta
     1501 ttgatagtag agaactaaat gatattttta aaagatttta cagaagtaaa aatgttgaaa
     1561 accaaaatgg cacagggatt ggtctatatc ttacaagatt aattttagaa aaagaaaatg
     1621 gaaacattat agtagaatct aaactaggaa gtggatgttg ttttagtatc ttcttacaga
     1681 attgtaagag tttaaactaa ttttgtagga tgagtgtaat tatcgtcact tacaatattt
     1741 tatagaacat atatctgtat tgaaaggaga aattaaatga gtatattagt gacgaataat
     1801 ttagtaaaac actatggaaa aggtgagact aaagtaaaag ccttaaatgg agtaagtata
     1861 gaaattgaac gagatacatt tactgctata gttggaactt ctggttctgg aaaaagcaca
     1921 ttgttaaata ttataggtgg attagataat ccaacctcag gtgatgtaat aattaaagga
     1981 aagaacatat ctaaaatagg aaaaaaagat ttgacagtgt ttagaagaag aaatatagga
     2041 tttatatttc aaaactacag tttaatgccc gtattaaaca cttatgataa tatagctctt
     2101 cctgttacat tagataaagg aaaccatata gaccatgaat atattgaaat gctgatgaag
     2161 acattgggaa tttgggataa gcgtttaaaa tttccaagcg aactatctgg tggtcaacaa
     2221 cagagggtag caattgctag agctttggca aataaacctg cgcttatatt agcagatgaa
     2281 ccaacaggaa atctcgatag taaaactaca atggaagtgg tatgtttatt aaaagaaagt
     2341 agtgcaaaat tccatcaaac gatacttatg gtaacacaca atgaaaatat agcacaagta
     2401 tgcgactcaa ttatccatat tgaagatgga gttgtggtta atactggtgg tgaattacta
     2461 tgatgtctct agtaaaattt tcaattcaat atataaagca ttataaaaaa caatctatat
     2521 ctatagtttt aagcatagta ctatcagtag cattattgac gggaattggt tctttagttc
     2581 atagtgcaga caaaagtaga attgaaaaga taagggaaga tagtggagat tatcattatt
     2641 tctataaagt agataaggaa caacttaata aaattaaaga aaataaaaaa tcaaaggaat
     2701 acactgtaga tagacttggc gttacaaaac ctaaaaatca gatagatgaa ccttttatta
     2761 tagaattttt aaatgctgat tcttcatatt tagacatgac aggtagaaaa ttattaaaag
     2821 gaactcttcc tagtaaagat ggagagatag tacttgacac atattctcta aacaatttaa
     2881 atgtaaacaa agaaattgga acagaaatca atttagatgg taaaatctat aagttatgtg
     2941 gtattttatc aagtctttca aacccagaaa ctgctctgga gggatttgta aacgattatt
     3001 ttcagcctaa agaaaaggat tcttatatag tttatgtaaa atatgatgaa aataaaaata
     3061 ttaaaaagca aaattataaa tttatgaaaa aattcaacat atcaaaagaa aaaacttata
     3121 taaactggtc tctttcagtt gaatttggag taaaaccacc aaaaaatata gaaaaatata
     3181 atatatttga gttcttaaaa aatttagaac taaatacaaa tgcaattata ttgcttgttg
     3241 gagtgtttgg tgcagcagca gtatatagta ttttccatgt ttcaatactt cagagaatat
     3301 cacaatatgg ggttttagaa gtattaggcg ctaataataa acaactttta tttctactat
     3361 ttttagagtt atttttactt tttattgttg gatttcctat aggttgcttc ttaggtattg
     3421 gagtagcgtc tacaatatat gaacaatttc cacatatatt tttgagtagt gatattgttc
     3481 caggagcatt ttttataagt gaaaaatcta tttatttagg ctttttattt atcttattgt
     3541 tgttaatttt aattgctgta cgtgttgtac atcagttgaa taaatactct agtatagaat
     3601 ctatgaaaaa ctttaagatt actaataaga aaaagagaat attagaaagt gtaaaacact
     3661 ctaacatgac aaaagtatta tctcataaat acatgactca aaaaaaggga atgtttattg
     3721 gaattttgtt ttcattatca cttggtggaa ttatatttct atgttctagt tactctatac
     3781 agcttactaa aaataataat gaacttacaa tgaaagctga tgatggttta aattctgact
     3841 accaaataac tatgcaaaca acagattttg atgtaggtat accaaaagat aaaatttcaa
     3901 gattaaacaa tgttgaggga atatcaaaag tctatccagt aagatattta tttggagcta
     3961 tgcaaattaa agaagaacaa ctattttgga aaaatttctt taaacctctt gaaaaagatt
     4021 acaggataaa gacttttttt gatggtattt gtacaaagca aaatgaaggt ggctatcttt
     4081 taaaaacaaa tatttatggt tataacaatg gtatgttaga aggattaaat tcttatatta
     4141 tagatgggga gattaatacc catgatatgg ttaaaaataa taaggttata gttagactac
     4201 ctatggatgg aactgggatg tatgatgcag ttgatataaa tccaggagat acaataaaag
     4261 ttaaagtgcc aaagacaatg aaaccaacag atgaaattgt taaatttaaa gaagaaaata
     4321 agaatgatta tacaacaaaa gaatttgtag ttgctgctac tgtaaaacgt gtgatggcaa
     4381 ataacatata tttcattggt gattatggta tggatatagt tatgacgaat gggcaaatga
     4441 aaaataactt tgatatcgaa aattataatt cagtaagtat aaaaaagaca gaagatagta
     4501 acagtgagga attgtctgag aacataaaaa gtgttgtaag tgatgtaaaa agatgtatcg
     4561 taacagatta taccattgct attgaaaaaa ataataccta cttaaatcaa aaactcttat
     4621 ttatatatgg tattgtattt gtacttcttg caataagttt gttccatata atcaatacag
     4681 taagctactt aattttttca agaagacatg agtttggaat tttaagagct atgggtatta
     4741 cggataataa gtttttactt atgatgataa gagaaggttt tttatatgga ttctatgcaa
     4801 gtattataat ggtaattgga agtgttattg gacaatttat gatttatttt atggtaaaac
     4861 gtgtatattt atatataaat ccaattctta aaataaatac acctctatac ataggtatga
     4921 ttattttaaa tatcactatt agtatagttg ctgtaattat tccagtaaga caaattttga
     4981 agagtgatat tatttcagaa ataaataaga attagagtta tataaatttt taagattata
     5041 taactttctg acaatcttgt ttaaattatt taaatatatc ttatctgtga acattaaata
     5101 aacctcttaa tagatataac tcttgaaaag tcatatctat taagaggttt taataaattt
     5161 tcatgttaat atatataatc caaaaaaatt aaatcttatt aaaacatact attttgtata
     5221 gttatcgaaa tatccttgta tgtaaataat aggagtaccc ttatctccac ttccagatgt
     5281 taaatctgct aaagaaccta taaggtctgt taatcttctt ggagtagtac cttgagaaac
     5341 catgtttcct gttaaatcat cagatttatt atctttttca actatgtatt ttgaaattgc
     5401 ttcttttaac tcatcaccag ataagtcagc aaaatcatta tctgctaagt attttaattt
     5461 tacttcgttt ggagttccct ctagcccttt tgtataagca ggagatacta ctgggtcagc
     5521 taattcccat attttaccta cagggtcttt aaaagctcca tctccataaa ccattacttc
     5581 tacatctttc cctgttattt cttttatatt accttggatt ttgtttacaa cttcatcaca
     5641 atttataggg aataatttta ctgtttcttc tgtagctttg tttgaaccta aaagtccata
     5701 ctggtcattg taaccactac catctacact tgctgtcatt atatcatcaa gtgaatatac
     5761 ttttttagct ccattttgtc ttaaaattct ctttgttctt tgtcttgtat gtatatcaca
     5821 agttaaaata cttgttgtat agttaagaat agtttttgga ttgtttgcaa atactatttc
     5881 tacctctgca ccacaatcct tgattaaatt cttataataa tcaacataat ctacaccagt
     5941 aaatgtatgc ttgttatatc caaatagttc tctgtatttt tcttctgtta aaacatctga
     6001 ccaaggattt attcccttat catcaagttc atcaatactt attaagtgat ttccaacttc
     6061 atcagaaggg tagcttaaca ttaatacgac ttttctacat ccttttgcaa ttccttttaa
     6121 acatatagca aaacgatttc tacttaaaat tgggaatata accccaacag tatcatctcc
     6181 aaatttatct tttacatctt ttgcaatatt gtcaacgtga gcataatttc cctgtgctct
     6241 agctacaaca gcttctgtaa cggcaattac atctttgtcc ctaacttcaa agttttcact
     6301 ttttgacgca tttaaaacag aatcaacaac tattttaact aagtcatctc cttgtcttat
     6361 aataggtgct ctaacccctc ttgaaacagt tccaactaat ctatccatta tctaaaatcc
     6421 tcctaaagaa ttaatattgt catattagta ccatgtatat tataattcaa atataattat
     6481 aagtaaaata aataatacta atatttgaca taaggagtaa ttatatatga atataaaact
     6541 tgaactttat aaggttttta atgcagttgt aaataataag agtttttctc ttgctgctaa
     6601 ggaattgttt atgtcacagc cagctgtaag ccagtcaata aagcaattag aagaacaatt
     6661 agatacttta ttattttata gaaacaataa gggagtaaaa ttaactccag aggggaaaat
     6721 tctaagtgaa catgtcacta ctgcattaaa gttaatatca tcaggagaag atagaataaa
     6781 taaatttaaa aaattagaat acggttctct gaaaatagga gtaggagata cagctgcacg
     6841 ttttttcttg ttaaaatatt tagaaatttt tcataagaag tatccacata tacatgtatc
     6901 tacaataaat agaacgagta gagaacttat atctttactc aaagatggga atatagatat
     6961 agcaattata aacatgccaa tagaagatga tacactaaat attgttgagt gtattgaaat
     7021 acatgatata tttgtatgtg caaatgatta catagaatac aaaggtagga aaatatcatt
     7081 ggaagaatta aacacattgc ctttaataat gctagaaaat aaagcaaatt caagacttta
     7141 tgtaaatgaa tattttttat caaaaggtat aaaactaaac ccggatatag aattgggttc
     7201 tcatgaatta cttttagaat ttgcgtatat aaatcttgga gtgtcttgtg ttatagaaga
     7261 atttagcata gattatttgg aaaatgagaa attgtttaaa ttagatataa aagagccaat
     7321 acctaagaga aatataggtt actgccattt aaaagatata tctttatcct tagcaaccaa
     7381 agaattttta agcatgatat ctaataatat ataatttaga atacatattt cacaagtaat
     7441 ttaatataaa aatagagata aattcgtaaa tttgaaagat tcatatagta attagttttt
     7501 attaaaaata aaaataaggt taatttgaga acatttatat aattaatatc gtattttaaa
     7561 tatgaacagt atatttagat taatgtataa agggggatta gaaatgtctg attctgaata
     7621 tcctcaaatg aaaaccttag aaactagaaa ttgtatacta agacctgcat cattagatga
     7681 tgttgaagat ttttttgact gttataaaaa taaaatagta gtaaaacatt taccatttag
     7741 tgagcataaa tctttagaag atacaagaaa gtttataaaa tcattttttc taaatccata
     7801 taaaaaaggg aaaattggcc actttgctat agtatataaa agagacaata aagttattgg
     7861 gaatatgggg tttaataata taaatcctaa agcattagaa gcagaaatag gtatatgtat
     7921 aaatccaagc tattggggac atgattttgc tactgagata acaaaaagag taatacagta
     7981 tggatttaga gaactaaact taaataaaat aattgcaata acctatgaag aaaaccaaaa
     8041 ttctacaaaa tcattagatt tattaggatt taaatgtaca ggaaagtata tcaaaaaaat
     8101 tcatactgga aatactgtga aaaatgttcc ttgttatcaa tatgaattga aaaaacaata
     8161 gatataatca tttaatatct atgctggtta tttagtaatg attgttttct aaataaaatt
     8221 tcaataaagt ggttgcctca aaatatagat aatcctgttt ttattgttaa aatactgaaa
     8281 atgcatttga ttttatacaa aaaagagtgt ctcatgaact aaaaagttta tgagacactc
     8341 tttttgaata aatcgtattt taattaaaat agtatatata cttaaaatat taatttttat
     8401 tagttctcta aatttttaaa tccttctcca agaacctcat gtacatctgt aacaataaca
     8461 aatgcagtag ggtcagttct tttaactaat ctctttagat aaagttcttg ttttttagaa
     8521 actacgacta atagaacttc tttattttct ctagtgtatc cacctttacc atcaagaata
     8581 gtaagacctc tatcaagttc ttcagttata gcttgtctta aactttcagg ctcatcagag
     8641 ataataaaaa atgcttttga atggtcaaat ccttccatta tcatatctgc tatttttatt
     8701 aatacacaaa gtgctatacc agagtaaaga gcagtttcta aactacgatt tactatacca
     8761 gaagatataa caaccattcc atctaggcat gacatgaatt ttgtagcttt tattcctggg
     8821 aatttcttac taagtatgag tgcaattaag tctgttcctc cagtagaagc atctgattta
     8881 aacattattc ctaaacctat accaactagg attcctcctg caatagaaga caataataag
     8941 tcattagtaa atcttagttt tgataatggg tctgttaatt gaactatagc tgaaaataca
     9001 actgttccaa ataaagtttt taatgaattt tttgtaccca taattctaaa agcaagtatt
     9061 actaatggga cacctataat taacatgata gctgatactg gaagtcctgt aaccttgttt
     9121 agtactaatg atagaccact aagtcctcca ggagcaattg tatgtggttt taaaaacata
     9181 tttataccaa tactcattga tatacaacca aaaaatagtc caagcatttc aagtaaaatt
     9241 ggcgactttt gttttttcat ataaacacct ccatgaactt gtcacaattt aatattataa
     9301 catatttgac cagtttatat tatataaaat ttcttgatat aagcaatttc aagtatttca
     9361 ttattcttaa tatttttgaa tttttatata gtgttttcat atttttactg atgttgttac
     9421 tctcctatga agtttttata tattataatt tttttattct tttataaagt atcccctata
     9481 aagatggttc tatgaaaatt ttctctatac tgtttaacgt atatagagat tttgtgtata
     9541 attataaggt atttaatgga aaaatttatc ttacttttat atgttatcaa aaggatttat
     9601 taaatacaac agatagcttg ataacatata attatataa
//
LOCUS       DBGJFU010000139         9539 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.140, whole genome shotgun sequence.
ACCESSION   DBGJFU010000139 DBGJFU010000000
VERSION     DBGJFU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.140"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            253..1182
                     /gene="glsA"
                     /locus_tag="ACOIAS_003178"
     CDS             253..1182
                     /gene="glsA"
                     /locus_tag="ACOIAS_003178"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGZ2702868.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1280..1843
                     /locus_tag="ACOIAS_003179"
     CDS             1280..1843
                     /locus_tag="ACOIAS_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702869.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2331..3167
                     /locus_tag="ACOIAS_003180"
     CDS             2331..3167
                     /locus_tag="ACOIAS_003180"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008833 - deoxyribonuclease IV
                     (phage-T4-induced) activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGZ2702870.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            3451..4446
                     /locus_tag="ACOIAS_003181"
     CDS             3451..4446
                     /locus_tag="ACOIAS_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGZ2702871.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4703..5107)
                     /locus_tag="ACOIAS_003182"
     CDS             complement(4703..5107)
                     /locus_tag="ACOIAS_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGZ2702872.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            5948..6862
                     /gene="queG"
                     /locus_tag="ACOIAS_003183"
     CDS             5948..6862
                     /gene="queG"
                     /locus_tag="ACOIAS_003183"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGZ2702873.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKNRQQKGYLTG
                     MEEPIIESRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            6968..9205
                     /locus_tag="ACOIAS_003184"
     CDS             6968..9205
                     /locus_tag="ACOIAS_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2702874.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTKQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
BASE COUNT         3882 a          856 c         1770 g         3031 t
ORIGIN      
        1 tgttgggtta gaaatttttg gtgttataaa aatttaaatt actggattat tagattgctg
       61 gattgctaaa ttactaggtt gttaaattat tgaattacta aattattaag ctctttaaac
      121 tatttaaatt gtttgaaaaa tgaattgatt cttgtagaaa ataattttaa agtataattt
      181 tttttaacat tgttttatta tagtataaac ttgtgctaaa atagaatata taacataata
      241 taagaggtgc atatgaatac gttagatgag acattattaa aagaaattat aagttctaat
      301 aagaagtata ctaattatgg acaagtcgct agttacatcc cagaattaaa aaacgctaga
      361 agaaatgatt taggaatttg tatcatagat agcgaaaata atctttatag tgctggaaac
      421 tgtagcacaa agtttactat acaaagtatt tcaaaaccaa ttgtgcttgc tatggcactt
      481 atggataatg attgggaaga cgttttttca aacgttggaa tggagccaag tggggaccca
      541 tttaattcaa taatgaaact tgaaattaat gacacaaaaa aaccctgtaa tccaatgata
      601 aatgcagggg ctatagtaac cacttccctt ataaatggga gttgtttaga agaaaaagaa
      661 gaaagaatgc ttagtttctt tagaaagtta gcaaaaaatg ataatatagg aataaactat
      721 gatgtctata agtctgaaaa aatgactgga gatagaaaca gagctatggc ttacttattg
      781 aaaagtgatg gatttatcag agggaatgta gaagatgttc tagaccttta ttttaaacaa
      841 tgctcaattg agatagattc tgtagattta gcgagaattg gaataaatct tgcaaattat
      901 ggagtagata tagaaaatgg tgagcattta atgagtgaaa tggtttctag gattgtcaag
      961 acatttatga tgacttgtgg aatgtatgat gcttctgggg aatttgctat taaagttggc
     1021 attcctgcaa aatctggagt tgggggtggt ataatggctt ctgtgccggg acgcatgggg
     1081 attggtgttt atggacctgc gcttgacaaa aaaggtaaca gcgtcgcagg agttaaagtt
     1141 ttagaggagt tgtcaaataa actcaaatta aatatttttt aaaatattaa gaaaggattt
     1201 ttttgacaac aacatataat ttaatatata attaaattgt aaagaaatat gaattaaata
     1261 aattttttag gaggggtcaa tgaaagaggt attggtatta cgagatttgg agtgcataaa
     1321 ggctattgcg catccacgta gaatagatat tctaaaagca tttgacaaat cgccattgtc
     1381 agcaaaacaa ttatctcaat tattggaaga acctcatgca aaaataaact accatataaa
     1441 aatgttatac aaagttggta ttttagaatt agttgaggaa aaaataaagt cggggattgt
     1501 ggaaaagtac tattatccaa gtgcaaaaaa tgtggttata gggaatagga tacttaattt
     1561 ttctttagac aatggggaag aaaaagaaga attgtacata tcaaaatttg aaaatatgag
     1621 tgaagttttt tataaggcag cagaggaaga tgttttagaa aatgaaaata tagttgatta
     1681 tcatgatatt tctcttactc atgatgaatt agttgaactt agtgacacta tgaagtcaaa
     1741 gatagatgaa attttaaata aaagacaaca caatgttgaa ggttctaagt atgatatagc
     1801 tatggttata gttcctactc ttgaggagga atgtcctagt tagagaatta attatggatt
     1861 aattaaagat ataatattag agattgaaca tcaaaattta cttaataatc aaaaaatagt
     1921 ttttggtatg ggtaaaacaa tgatttttaa tctctatttt tttgtttttt tatacctatt
     1981 gtatttgtaa ataatatgta tagtgtaaga tgtatacact atctaagaag tgcttagata
     2041 atatacaaag tatgtaagat tataatattt gaaattgatt aaaatgggta taattaaagt
     2101 gtaaatatta aaattaaaaa ttaatataaa ttaaatcaat gctagaaata tgttaaaaga
     2161 ggactgagat tagttgggat agaacatccc ttagagctta cgaattttta gtgaaaaagt
     2221 actatcaaat aggaatctcc atcttcatat gttggctaaa tgtagttaag gtggagtagt
     2281 tcaaaattaa aattgagtac aagtagtagt acaaataaaa ggagattaat atgattaaat
     2341 taggaacaca tctttcaata gcaaaaggat ttgctaatgc ggctgagaca gctgtatata
     2401 ttggtgcaaa tacatttcaa ttttttagta gaaatccaag aggaggaaat gctaaagaat
     2461 ttgatgaaaa agatataatt aaatttcaag aaattagaaa agaaaataat tttgcaccac
     2521 tgcttgcaca tgcaccatat actatgaatc tgggtggtac aaaggatgat gtatacgaat
     2581 ttgcaacaag ggtgatagat gaggatataa gaaggatgga ctcattagga attgaataca
     2641 tgtgttttca tcctggaagt catgtaggtg gtggtgtaga ttttggcata gatagaatag
     2701 taaaaggatt gaacaatgcc ataaagggtg atgaaaatat aactatactt cttgaaacta
     2761 tgtcgggaaa gggaactgaa ataggtttta aatttgagca attaaaaagt ataatagaca
     2821 aaattgagca taaagaaaaa ataggtgtgt gtcttgatac atgtcatatt ttttcagctg
     2881 gttatgatat agtgaatgat ttagatgggg ttttggaaga atttgataag gttataggca
     2941 tagaaagatt gaaaactgtc catctaaatg atagtatgat gccatttgga gataaaaaag
     3001 acagacatgc tcctataggg gaaggaaaga taggattaaa agcattaatt gattttatgg
     3061 aacatcctag tctaaaacat ttaccatttt tcttagaaac tccttttgat gatgaaggtc
     3121 acaagaaaga gcttaaaatg ataaaagaaa tactatctag taaataaacc tttattaaaa
     3181 atatgtgaaa tacttcaccc tcaacataat tagggtggag atacaatcat gaaatgtgat
     3241 aatttaaatt aatggatagg aataacaaaa attgtataca aattaactgg gtataaagta
     3301 tgattatatt atgaatagac tgatataaaa tataattata ctatgaatag tatgtaaata
     3361 tattgcaaag attctcataa tttgttgtat aattaaagat gacatattta tattatataa
     3421 aaaattatta aaagttaggt ggggaaagat atgagaaatt taggcaatac taacatgaaa
     3481 ataaagagag ttggctttgg gggcattcca atacaaagga ttactcaaga tgatactaac
     3541 ttagttataa atgagttaga aaaacaagga ataaacttta ttgatagtgc aagagggtat
     3601 acaataagtg aggaagcaat cggaattgcc attgaaggaa aaagagataa atttttctta
     3661 gctactaaaa gtatgtccag agattatgat tcaatgaaga gagatgttga aataagttta
     3721 aataatttta aaactgattt tatagattta tatcaattcc ataatgttaa agaagaggag
     3781 tatgataatc tttttaaaga taaaatggct tatagtgccc ttttagaggc aaaggagcaa
     3841 ggtaagataa aacatattgg tataactagt cacaatttaa atactataga aaaagctata
     3901 gaagatggaa aatttgacac tatacaattc ccttacaata tagttgaagg acaagcagat
     3961 gaagtgttta aaaaagctca tgaaaaagga attgggataa tagtaatgaa acctttagct
     4021 ggaggtgctt tagacaatgc aacactggct attaaatata tactatctaa agattacata
     4081 gatgtagtta taccaggtat ggagagtgtt gaacaggtta gacaaaatgt cgctgtactt
     4141 gaaaatcttg tgctagatga aaaagataat aaggaaattg aagaaataag aaattcttta
     4201 ggtaaaaaat tctgtagaag atgcgaatat tgtatgcctt gtgctgtagg aataaatata
     4261 ccattgagtt ttttatgtga aggttattat actagatatg gattaaaaga gtgggctaaa
     4321 gaaaaatatg aggttatgga tgttaaacct actgagtgta ttgactgtgg gctttgcgag
     4381 agtagatgtc catatgaact tcctataaga gaaatgctta agactgttgt tgaaaagcta
     4441 ggatagattt tatttattga aatcggtgta ttaaatttac tccgatttct ttttattaga
     4501 tgtggtctta tatacaaata ttaaatttac atagttatag ctattatata aatttaatat
     4561 ttgtttaata cttattaaat atttacttaa tgctttttaa aaacatttgt ttgagtatag
     4621 acataacaca attattattg ttgtattagt atagtttcaa atactggagt atcaatataa
     4681 tgtcgaataa cacataaaat ctttattttt caaaaatatt ataggatata tcgtatttag
     4741 atactggata aagttcaatt tcaaccctag ggttatcttt atctataaaa tcaaataaaa
     4801 gtactggctt aagttgttta tcatttttta ttattccact tttttcaata ccatcacaaa
     4861 tactttttgg ataattatgt aaatccccca ttctcttatt tgggaagaat aatttcaaga
     4921 ctgttattag attttcttca acagtttccc cttgatactg ttggtttata aaacctgcaa
     4981 tcatgttttc gtatgcaaga tacttagaat attttccctt tgaaggcatc catgcttgac
     5041 catttatgtt atgaagctta aaattagatt tactaatagg tctacctgga attgttattt
     5101 taatcaaatt tttcaccatc cttatacagt atttattata atctgtatat gatttaaaag
     5161 caaattgtta tttaaagtgt ttttacagag cttgtttgta aaattttcca ctttagatgt
     5221 agtaatataa gataagtatt ttgcttacaa attttaaaat ttgatatatg atgggcatat
     5281 tcatattaga ctattataag accttcataa tattaataag tataagttta gtaaagagag
     5341 taaaaagaaa tccatataag tcaagtaaag acttaataaa tggcaatgta aatgggatat
     5401 taattataag aaaaaatcaa tctttttgct taactataga aagaatgtca aaatataact
     5461 aatgatattt gaagttttag agtttatatt aaaaggtaag aaggttaagt gggtaaaaaa
     5521 ggttgaatag attaaaaagg ctaagtatct aaaagagtta agtaggtaaa aaagttaaat
     5581 aagtaaatga aaaaggctaa gtatctaaaa gagttagtag gtaaaaaagt taaataagta
     5641 aataaaaaag atgaagtatc taaaagagtt agtaggtaaa aaactaaata ggtaaaaaga
     5701 tgaagtatct aaaagagtta gtaggtaaaa aattaaatag gtaaaaagac gaagtaccta
     5761 aaagagttag taggtaaaaa aattaaatag ataaaaagac gaagtatcta aaagagttaa
     5821 gcaggtaaga aaattgaata gctaaaaaga ttaagtattt aaaagagtta gtagctagaa
     5881 aaggttaaaa agactaaaaa aattaaataa atttaaaaag tcaattagat taaaaaggaa
     5941 taattttatg gataataagg aattaaaagc attttgtaat ttaataggat taaactgtgt
     6001 aggagtagca ggggtagata aatatgataa tttagaaaag atacttaaga acagacaaca
     6061 aaaaggttat ttaactggta tggaagaacc aataattgag agcagaatta acccaagaca
     6121 tataatgaag gatgctaact ctataatagt ctgtgcattt ccatatcata taaatagaca
     6181 agaaaagaat aaaaattcaa atttatcaaa atattgtcat ggaaaggatt atcacattgt
     6241 agtaaaggac tttttacaac aaatttgtga ttatatttct aaaacagtaa cagattttaa
     6301 atacaagtta tttgctgata atggaccatt agttgacagg tatttagctt atttatcagg
     6361 aataggatat tttggaataa ataataatat cataactgat gaatatggtt cttacgtatt
     6421 tatagggtat atagtaaata attataaatt tgaagaagat aaaccactag aaaaaagttg
     6481 ttttaaatgt ggtaagtgtg taaaatattg tccgggaaat gctatattgg gaaattatga
     6541 tatgaacccc aaaaaatgtt tatcatatat aactcaaaaa aaaggagatt tgtcagagaa
     6601 agaaaaagtt gttttaaaga atggcaaaaa ggtatttgga tgcgatattt gtcaagaagt
     6661 atgtccacat aatgttgaaa taccaacaac tcatattttg gaatttaaag aaaatataat
     6721 agattatttg gattatgatg agatacaaaa tatttcaaat aaagaattta aaaggagata
     6781 tggagataga gcttttagct ggagaggtag aaatataata aagagaaata tggaaataat
     6841 tttagataag tatgatgact aataataaga gtctgtttca tatactttaa tgtgtattta
     6901 ctttaacact tgttgtgtaa aagtcatatt atatcattaa agttaaatat ggggaggtgg
     6961 gggaatattg aaatataata atacatctga aataaatcca ctagattata tattgggtca
     7021 agaaagagct gtagaagcta tggaattagg gttgaaaata aataatccag cgtacaacat
     7081 atatatagct ggtgaacctg gaactggtaa aagtacttat gccttaaagg ttttaaatga
     7141 gtatgcatcc aaaaaaaata cgcataaaga ttggtgttat atatataatt ttgaaaaccc
     7201 tagagagcct attatagttg aattagaaaa ggggtttgga agagaactta agaaagatat
     7261 ggagaaatta attgaaagtc ttttagaaga ttttaaaaat gcgtttgaaa gtgaaaattt
     7321 tgaaatagaa aaaaataaac ttctggatga atatgaaata gaaaaagata tgcttttgaa
     7381 gcaaattaaa aaatatggag aagaaaaagg atttaaatta aaacagagta aaatgggaat
     7441 agtttttatc cctttgaaag aagaggaaga tgaaagtgat gagtctgatg aagagtttta
     7501 caaagctaaa agagaactag aaaatatggc gatacaggtc gtatataaaa ttagaaactt
     7561 agaagaaata gctgaaaaag cagtgttaga gcttgaagaa gagatagcta aacttgtagt
     7621 agaacctcat atcaagagat tgtgtgaaaa atatgaaaat tatgataaga ttcaaactta
     7681 tctggaaaat ataaagaaag atataataga atatatgtat ttattctatt tagatgaaga
     7741 ggagttaaaa gataagtatg ataaagagca ttttattaaa tataaaatta acctatttgt
     7801 agacaatgga agctcaaaga ataaagaatc agcacctgtt gtagtagaga taaacccaag
     7861 tccagcaaat ctatttggaa aggcagagta tgattatgca aatggaaata taaagactga
     7921 tttcacaaaa ctattatcag gagcatttca tagggctaat ggtggttatt tagtacttta
     7981 tgcagaccaa ttactaaaat atacaatgtc ttgggaaatt cttaaaaaga ctttgcagac
     8041 taaaaaagta atattggaaa cacaaacagc aattaaacca gaaaatatgc cattggatgt
     8101 taaattagta ctgattggaa gccactatat atatgatatt ttatatagat atgatgaaga
     8161 ttttgaaaaa tattttaaag tatttgtaga ctttgatagt gaaatggata aaaatgaaga
     8221 taatgaagaa ggaatagcaa gatttatagc ataccaatgt gataagaata atttaaggca
     8281 ttttactaaa tcagcagttg aagaggttat taaatttagc actagattat ctggtgattt
     8341 agaaaaatta tcaactaagt ttaataaaat aatggaagtt gtaatagaag gaagtgctta
     8401 tgcagagttt agaaattcgg agtatacaaa gcagtgtgat gttaaaaaag caataagtga
     8461 aaagagaaaa agaatcaata gagtggaaac acatatggat gaatcaattg aaaatggatt
     8521 tactctaata gaaacagagg gaagaaaagt aggagtgata aatggtttgt cagtcttaag
     8581 tacaggagaa tactcttttg gtagagcttc aaggattaca gcgaccacta gcccaggaag
     8641 taaaggcata gtaaatatag aaagagaagt aaatatgagt ggctctatac ataacaaagg
     8701 agtacttata ttaggtggct atctttctga gaattttgca caggatttac tattgtcact
     8761 aaatgcatat gtttgttttg aacaaaatta tggtggaata gatggagata gtgcatcttc
     8821 tgcagaatta tatgtactat tatcatctct tagtggagta tcaattaaac aaaatattgc
     8881 tgttacaggt tctataaatc aaaagggtga tatacaagta gttggtggta taagtgaaaa
     8941 gatagaagga ttttattcta tatgtaaaac taaagggtta aatggtgagc aaggcgtcat
     9001 aattcctaga aaaaatcaaa gaaatctcgt tttatcagat gaagttaatg atgcagtgcg
     9061 agatggaaaa tttaagatat atactgttga aagagtagaa gaggctatag agatattgac
     9121 agatgttaaa tttgaagaaa taaagaaatt ggtaaaagaa aaattaatat ctttcagcaa
     9181 gatacaaact gtgtcaaaag aataagttta ttatagaatt taatgggtat ttatgtataa
     9241 taaaaagcaa gaactttaat aggtttttgc tttttttgta aatatatata aattaaattc
     9301 ttattttgta tgttttatat attgatgttt atttttttgt tgatatgatt aaactattta
     9361 taaatagcgt tttataaatt ggtattttga agggggataa ttatgaaaaa tgtaaatcta
     9421 aaagagataa atgaaaaaga tgtaattttg gaagaagaga taaataaaaa aggaatggat
     9481 aagaaaaata taattgagaa ggaagagata aataaaaaag gaatagataa gaaagatat
//
LOCUS       DBGJFU010000140         9484 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.141, whole genome shotgun sequence.
ACCESSION   DBGJFU010000140 DBGJFU010000000
VERSION     DBGJFU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.141"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            382..1368
                     /locus_tag="ACOIAS_003185"
     CDS             382..1368
                     /locus_tag="ACOIAS_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGZ2702875.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1704..3185
                     /locus_tag="ACOIAS_003186"
     CDS             1704..3185
                     /locus_tag="ACOIAS_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGZ2702876.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3211..4182
                     /locus_tag="ACOIAS_003187"
     CDS             3211..4182
                     /locus_tag="ACOIAS_003187"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HGZ2702877.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4438..5040)
                     /locus_tag="ACOIAS_003188"
     CDS             complement(4438..5040)
                     /locus_tag="ACOIAS_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2702878.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            5321..6532
                     /locus_tag="ACOIAS_003189"
     CDS             5321..6532
                     /locus_tag="ACOIAS_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGZ2702879.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6600..6797
                     /locus_tag="ACOIAS_003190"
     CDS             6600..6797
                     /locus_tag="ACOIAS_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HGZ2702880.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6831..7802
                     /locus_tag="ACOIAS_003191"
     CDS             6831..7802
                     /locus_tag="ACOIAS_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2702881.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            7989..8366
                     /locus_tag="ACOIAS_003192"
     CDS             7989..8366
                     /locus_tag="ACOIAS_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2702882.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            8897..>9484
                     /locus_tag="ACOIAS_003193"
     CDS             8897..>9484
                     /locus_tag="ACOIAS_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HGZ2702883.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLL"
BASE COUNT         3822 a          956 c         1479 g         3227 t
ORIGIN      
        1 acatatgaaa ataaagttta tatagtttta aaaattatag aattttaaaa tatttatata
       61 tattctttaa ataaaaaatt attttttaag tgataattaa gtgtttatcg ataaggctat
      121 ggttgtaaaa atcatagcct tatttttata ttataaataa attagagtat ttatcaattc
      181 atagtagata aaattgaaat taaatataac aacaaaaaat gaaaattaca attaatttaa
      241 tgttaaagac tcaattattt ggatgtggaa tggatagtac aagtagtata taaaaagtat
      301 tgactggaca aataactata ttttatttta aatatataat ataatgtaat aaaatatatc
      361 aaatgtagaa ggtgataacc tatgaaaaaa ataacaataa atgatattgc taacttggca
      421 ggagtatcta aaagtactgt atcaagatat ctaaacaata aagatatcag tgattctact
      481 aaagagaaaa taaagacaat aatagatgaa tatggatatg agccgaatgc atttgctcaa
      541 agtttgagag ctaaaaaaac atattttatt ggtattataa caccgtgttt agattctttt
      601 gtaaaatcta aaattatgat ggcaatagat gaagaactca aaaaattaaa atatacatca
      661 ctaattatta atactaatag aaaaataagg tcagaaatag atagtatatc aaagttagct
      721 agtctaaaag ttgatggaat aatactaatt ggtaccgaaa taactaaaga acataagaat
      781 gaaattgaaa agttagatat accgatagtt gtagttggac aaaaagttga tggtatcaat
      841 agcatagtga atgatgatta tggagcaggc tataaaatgg gtcaatatat tgctaataaa
      901 ggttacaaaa acattgtata tttaggtgtt gatgaaagtg atatatcagt tggtttaaat
      961 agaaaaaatg gagtacttaa tgggttaaaa gataaagggt atgatgcaaa agtattttat
     1021 actgactttg accaagaaat gtctatacaa agaagtggag aaatgctaga aagtgaaaat
     1081 ccagatataa taatatgtgc aactgataat atagcaatag caacaatgaa agaaatcaat
     1141 aagagaggta aaagtatacc acaagatata tctgtagctg gatttggggg atatgatgta
     1201 ttgtctatta taagccctaa acttacaaca ataaagtttg aaaataaaaa tgcaggtaaa
     1261 gtagcagcaa atacgatagt aaatttaata caagaaagaa aagaaccact attaaaggag
     1321 attaaatttg aattaataga aggtgaaagt acaataaata aaaattaaga tacacattac
     1381 atatattagt aatgtgtatt tttttactaa aaaaactata attctaaaaa ttaggaaaac
     1441 ttttttgaaa gaaatattgc atctgaaata aatttatgct aatctttaat taaaccggtt
     1501 tcgaaaccgg tttaattaaa ccgatagaat agcaaaatga gtgcaaaaat aactgattgt
     1561 aacaaagtat caaagtaatt attggagtac ataggaataa acaaaatata aaagatgtag
     1621 cacattgtgt aataagacta agaattgttt tagatagcaa taataaagca aatataaaat
     1681 tataaaagaa aaggaataaa aatatgtaca aagagcctaa atatagaacc atattagaat
     1741 cgacaaaaga agaattaaat aaattaagaa atatatcttt aaatgataaa tataaaccac
     1801 tatttcatat acatccacaa catggactac ttaatgaccc aaatggatta gcatattata
     1861 atggtaagta ccatgtattt tatcaatggt atccatatga tgcaactcat ggaatgaagc
     1921 attgggctta tgtaagttct gatgattttg taaactggaa tagagaagat gtagcattaa
     1981 taccaatcga aagttatgaa tctcatggag catattcagg taatgcgata gaggtagatg
     2041 gaaaattgca tatgtattat acagggaata taaaatatag tgcagaagat agatatgcat
     2101 atcaaaactt agctataatg aacaaggatg gtaaaataac taaatatgaa aataatccaa
     2161 tagtaagtga aataccaaaa ggatatacag gacatgtaag agacccaaaa gtatttaaaa
     2221 gaaaggataa atactttatg ttacttggag cacaaacaag tgataaaaaa ggagttataa
     2281 tagtatatga atctaaaaac tctatagatt ggaattttaa aggagaatta aatgtaaaaa
     2341 acatagatga agattttggt tacatgtggg aatgtccaga ttatattaat atagatgaaa
     2401 aagatatatt aatattttca ccacaaggag tagaacctaa aggatttgat tatcaaaata
     2461 tatataatgt agtatatgct attggtaata tggatttaga taatttaaca tttgaaatag
     2521 atacaatgaa agaactggaa aaaggatttg atttctatgc tccacaaaca tttataaagg
     2581 actcacaaat aatactattt gcatgggctg gaatgggaga agttttatac cctacagata
     2641 aaaacaaatg ggcacattgt ctaactgttc caagaaaatt aaatataaaa aataataagc
     2701 tattgcaaat gccagtagat gaattaatta aattaagata tgatgaaaca agtgggcaaa
     2761 atactataaa gaataatatt aatattatag aaaatgatga aaatgtatat gagcttaata
     2821 ttaatataaa aaatattgac agcaataaat ttgggctgga attattttca tcacaagatg
     2881 aaggtgtaaa attagaattt aacaaattag gaaacattgt aactttagac agaagcaact
     2941 ttaaaaaagt tttcggtgtt gaatatggaa caaatagaaa agaatatata aatattgatg
     3001 aaaatactaa tatcaaagta ctagcggata gaagtatttt agaaattttt ataaatgaag
     3061 gagaagtagt atttactagt agaatatttg ctaaagaaaa ttctaatcaa ataagggttt
     3121 atagtgataa aatagtagag tatgagtata ctaaatttaa attaaagcaa ggtattgaat
     3181 tataaaaaca cattataagg ggtagaaaag atgaaaaaag taattagtat aggagaagct
     3241 ttaatagact ttatacctaa tcaaaatggt ggtaaattaa aaaatgtatc agaatttaga
     3301 agagtagcag gaggagctcc agcaaatgtt agtgctgttg tagcaaagct tggaggaaaa
     3361 tcgagtttta tatctaagtt aggaaaagat gcatttggtg attatataat agatgtatta
     3421 aatgaagtaa atgtaaatac tgattatgta ttgagaacaa gtaaagcaaa tacaggactt
     3481 gcttttgtat cattaaaaga agatggtaat cgtgatttct cattttatag aaatccaagt
     3541 gcagatatgc ttttagaagc tgatgaagtt aaaaaagagt ggtttaataa ttgtcatatc
     3601 ctacattttt gttcagtaga tttaatagat agtccaatga aattagcaca taaaaaagca
     3661 atagaatacg cacttgaaag taatagtata ataagctttg accctaatat aagattacct
     3721 ttatgggata gtgagcaatc ttgtaaaaag gcaatatcag aatttttacc ttttgctcat
     3781 attgttaaga tatctgatga agaattagaa tttataacag gagaaaatga tatagaaaaa
     3841 tcattacata aactctttgt ggggaatgtt gaactagtat tatatacaaa aggaaaagat
     3901 ggagtagatg catacactaa aaaagtaaaa ggtatgtgta aaggggttaa agtaaatgca
     3961 atagatacaa ccggagcagg agattcttat ataggttcat ttttatacac cttattgtat
     4021 aaacaaataa cattgaatga tataaaagaa atgaaacaag aaacgttaaa tgagtattta
     4081 gaattttcta attattatgc agctcgtagt acaactatta aaggtgcgat aagttcatat
     4141 gcaacaaagg aagaaataac tgaatttata aaaaatttat agtaggaatg agtaataggt
     4201 tttgaaggaa gtaacatgta aagagttttt aaggatgttt actatgaaaa taattaacat
     4261 ataaaaagca ataaggaggc tgtctcaaaa tagagataag cctctttatc attgttgaaa
     4321 tgataaaaaa tattttaatt attgttactt tttagaagaa tatatatctt attttaaata
     4381 agcaaattat gtaagattta tttatattat caatacttta cttggagtat tatgtcttta
     4441 gcagttatct aattcaattg aattagataa aatataataa atttgttttg caaattgacg
     4501 agaatgttct tctttttctt tattcataaa attaggttta cctaaatcac gttcaacaag
     4561 cattcctata tattttaact gtgaatgctt acagtagcgc ttcataccat tttcccaaat
     4621 atcagcaccc ttttcaggtc tatatccaca tgttgtaata agtgctactg attttcctgc
     4681 ccatagagcg gggcctttct cattaccata gtatttattc attccataca ccaatctgtc
     4741 aagtagtgct ttcattggag aagtgcagta ccaagaataa attggagtag caaaaacaat
     4801 taaatcacac ataagaattt tcttgaaaat tttctgaaca tcatctttac aataacatcc
     4861 aaaatcaaat aagttatcct gacacttttt acaagcaata catggctcta ttttacaatc
     4921 atataaccat attacttcaa tatttgaatt ataattttct aattcctcaa taaaaggttt
     4981 taaaatagca gcagtattac cttttttctt aggacttccc ataagaatgc atacttccat
     5041 attgatacct ccacagtatt ttaaaatagt ttttagtgct taatatagtt ttaggatact
     5101 atgagaatac gttaacaaca atacactttg tatatttgta ataaaattga aacaaattgt
     5161 tttatcaata aaagttggaa tgtatctata attaaaaata ttttaggtat gtttttacat
     5221 atatgtgtgt agttaactat ggtaatttat tgtaaaatta tcttatagga ttataattgc
     5281 ttaatttatg attatattta aaatcttgga ggtaaaaaat atgtactcaa atgaagacta
     5341 taaatttata gaagaaaatg caccatgtta tatatatgat gagactaata taattgaaaa
     5401 atgtaaggaa ttgaaaaata atattttagg ttttgatttt ttatattcta taaaaacaaa
     5461 tccatataac aaaattgtaa aaagcattgc agagcaaggt tttggagcag atgcttcttc
     5521 aactaatgaa gttatgattt catcaaaatg tggagtttct aaagatgata tttattattc
     5581 atcaccaggc aaaacagaga ttgatataga aactgtttta gataaatgca taattattgc
     5641 agatagtatt acagaaatag aacttataaa taagatatct aaaaagcata atcagaaagt
     5701 taaaatagga ataagaataa atcctgattt tactatggat gatgatagtg ctaaatcaag
     5761 caaatttggt atagatatag aagatatgag cagtgttttt aagatgttaa gtatgtgtga
     5821 ttatgtatct gtagttggga ttcatataca tattaaatct caggtattgg attttgaaaa
     5881 gttaggacat tattatgaaa aatgtttttt tatagcttta aatttaaata aaagtaagta
     5941 tattaatatt gaatttataa attttggtag tggacttgga gtagcttatg ataatcttag
     6001 agaaaatcca cttaatctta aagagctttc aaaattaact caaaagattg cttttactaa
     6061 ttcaaacaca ctaaaatcaa gacttcttat tgaatcagga cgtttcatag tttgtaaatc
     6121 aggtaattat tatgttaaaa ttgtagatat taaagtatca agagggaaaa aatatcttat
     6181 tgtaaaaaat ggacttaatg gatttatgag acctattatt tctaatttaa ttgaaaatat
     6241 tacttgtgga gtagaattag aaggtcaaga gccattatat acatcaaatc atgccttttc
     6301 tctttcagta ttaaataatt taaaagaaga agagtatgta aatgttgttg ggaatctctg
     6361 tacatcatta gatgtaattg caaataatgt taaattgaaa aaagcaaaca taggagattt
     6421 gatttcaatt agtaatgctg gaagttactc ctattcttta tcaccgctat tattttcaag
     6481 ttatgaattt cctaaacaat attataaaag aaaagatgga tatattgagt aaacaatagc
     6541 ataaattaat aaacttagta ttattgaaaa aatttaaaat gcagggggtg taattctata
     6601 tggaattgtg gaaaagaaat ttatttgtat gttggatagg tatgtttttt agttctatag
     6661 ggatgagcca aatagcacct atacttccat tatatattaa acagttgggg gtaactgatg
     6721 ttagtttaat acaacagtat tcaggtataa tttttggatg tacctttgta gtagcagcat
     6781 tcttttcgcc aatttgaggg aaagctgctg ataaatatgg aagaaaatca atgcttttac
     6841 gtgcaagtat tgggatggga atagttatat ttaccatggc gtttgttcaa aatgtttatc
     6901 agttattagg attaagaata cttcaaggag tttttacggg ttatgctact gcatgtacta
     6961 ctcttattgc aacacagaca gataaaaatc attcaggatg ggctttgggt actcttgcaa
     7021 ctgcatcaac tactggctca ttaattggtc caacagtagg aggatatata gaatcaatct
     7081 taggtcttaa aagtacattt attataacag gaggattgtt gtttgtatca tttatcatct
     7141 ctattttatt tgtagtagat aattttaaac ctaaagaaat aaaagaatca atttctatac
     7201 ctaaagaaca attaaatata ttgccaaata aatttttaat tgcatctatt tttgtaacca
     7261 cattcattac tcaactagct ttatactcta tagaaccaat tgtaaccata tatatatcac
     7321 aacttacaaa ttttgcatca aatgtagctt taatagctgg attgacattt tctgcatcag
     7381 gattagcaaa tttattagca gctcaaaaac ttggaaaaat gtcggataaa ataggtcctc
     7441 aaaaagtatt gttaatttca cttttatggg ctgggattat atttatacca caagcttttg
     7501 taaaaactgc atggcaactg atgttgttga gatttttatt agggttgtca gttgcagggc
     7561 ttaatccttc tgttaacagc ttgcttaaaa aaattgcacc agaagaatat gtgggaaaaa
     7621 tatttggata taatgcatct gcccagtata taggctgttc atcaggagca tttttagggg
     7681 gacaaatatc agcacattta ggtattagaa cagtattttt ttcaacaagt ttattgttgt
     7741 ttaggaatgc actttggatg tataaattta ctggattgtt tattaaagat aagactaaat
     7801 aatcatacta tttacatttc taattttatg atgtaaaata aatatttttt atgtaattta
     7861 tcatagaatt atataaaaaa taaccctatg gtaaaaattg gaaaatatta ttatcgacaa
     7921 aatctttgac ttaaaaacta tattgcgata gtattaattt aataaaagtt tgagaggggc
     7981 gtaaggttat gacaatttca aaaataccac aagcagaatt aaaagttatg aagtttattt
     8041 gggaaaaaaa tgatacagta acatcaaaag aaactattga agctatggaa cgaaaatatg
     8101 gttggaaaca aacaactaca ttgacacttt tatcaagact tgttaaaaaa cattttttaa
     8161 gtgcagaaaa aatagataga tacactcatt atacaatagt tgttggacat aaggagtatt
     8221 taagtgttga gacaaaagat tttttaagca atatacacga caattcttta cagagtttaa
     8281 tttcagcact acatgatgat gaagttgttg ataaagatat gttggattta tttgaaagtc
     8341 attttaaaaa tttagaagaa gagtaattat atcaggatag aactctaaga atttaattaa
     8401 tctatggctg aaaaccataa atggtattat ttaatcagct actcactatt cataaagtaa
     8461 tattaaatag gttctctaat tagtaaaatg cctaaaataa ttggaatagg gagtatcaag
     8521 ttaaatgata ccacttattt aaataagaaa tgatttttaa aatttaaaat aattttataa
     8581 cttgtcaata ttcattaatc taaagtatta tttctattta accatatcaa aatatatttt
     8641 actattttta attattacaa tataaacctg tcttttgtta ttataggtaa actaaaagta
     8701 agaaatgatt tttatatttt taaactttat tattggtaac ttatctgtaa atattttaaa
     8761 atgtactata gaaagttgtt aaaaatatta aataattata ttaaatttaa gtgtgttttt
     8821 tatgctataa ttagtatgta ttttttatga tatattttat aaaaaatata atttaatcat
     8881 ttttgaggat attattatgt tagatgcatt tataacaatt gtttcttcta ttatagaaat
     8941 atggtcatgt aaaaaagttt ttgattatac tagtcagtca aagaccgact taaaaaaact
     9001 aaatatgtat tttctctttt ctgtttttct cattatttct tctttatata taaatatagg
     9061 agctaatagt agaatcttga tatgtatatt tgtaatgttc gttttctata agtctaacta
     9121 taaggttaat atatataaat gtctggtaat ctcatttttt tattggttaa tattaatagg
     9181 tataaatgga ttaagtatgt tattagtagc aacagtaagc aatttggata atatgaatgt
     9241 tttacttagt ggcagtttat atagatatga agcaatattc ttgggaaaga taatgttagg
     9301 tatattactt tatgtttata aggcttcttt taaaaatttt aaaataagta aaaaagagat
     9361 atacatatgt attcctatga tagcaaattt attgtcattt tttgtacttt atgattatat
     9421 tattaatctt gtaaaaatct ctcctgaaag aagaactgct tttttagtaa tagttttact
     9481 atta
//
LOCUS       DBGJFU010000141         9428 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.142, whole genome shotgun sequence.
ACCESSION   DBGJFU010000141 DBGJFU010000000
VERSION     DBGJFU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.142"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            144..464
                     /locus_tag="ACOIAS_003194"
     CDS             144..464
                     /locus_tag="ACOIAS_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HGZ2702884.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            602..1246
                     /gene="trmB"
                     /locus_tag="ACOIAS_003195"
     CDS             602..1246
                     /gene="trmB"
                     /locus_tag="ACOIAS_003195"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGZ2702885.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1390..2121
                     /locus_tag="ACOIAS_003196"
     CDS             1390..2121
                     /locus_tag="ACOIAS_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702886.1"
                     /translation="MNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDANLRAVV
                     KRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEIPKEAL
                     SLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHNVDIAK
                     GESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGNKTGQA
                     VVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2250..2528)
                     /locus_tag="ACOIAS_003197"
     CDS             complement(2250..2528)
                     /locus_tag="ACOIAS_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702887.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            2803..3249
                     /locus_tag="ACOIAS_003198"
     CDS             2803..3249
                     /locus_tag="ACOIAS_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702888.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            3519..3971
                     /gene="accB"
                     /locus_tag="ACOIAS_003199"
     CDS             3519..3971
                     /gene="accB"
                     /locus_tag="ACOIAS_003199"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGZ2702889.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            3987..5348
                     /gene="accC"
                     /locus_tag="ACOIAS_003200"
     CDS             3987..5348
                     /gene="accC"
                     /locus_tag="ACOIAS_003200"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGZ2702890.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            5363..6217
                     /gene="accD"
                     /locus_tag="ACOIAS_003201"
     CDS             5363..6217
                     /gene="accD"
                     /locus_tag="ACOIAS_003201"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGZ2702891.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKLLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            6238..7182
                     /locus_tag="ACOIAS_003202"
     CDS             6238..7182
                     /locus_tag="ACOIAS_003202"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HGZ2702892.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            7371..7631
                     /locus_tag="ACOIAS_003203"
     CDS             7371..7631
                     /locus_tag="ACOIAS_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HGZ2702893.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            7784..8155
                     /locus_tag="ACOIAS_003204"
     CDS             7784..8155
                     /locus_tag="ACOIAS_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGZ2702894.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            8185..9030
                     /locus_tag="ACOIAS_003205"
     CDS             8185..9030
                     /locus_tag="ACOIAS_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702895.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
BASE COUNT         3690 a          974 c         1570 g         3194 t
ORIGIN      
        1 aaatattata taaaatattt taaagcctta ggtaatatta ggtagaagtt ttagtaaaat
       61 atgcaaaaac taattgatta tgtaccttgt atgggtatat aatagatatt gttttaataa
      121 aatatttgaa agggaatgat ttgatgaaaa ttactttacc agaaacagct attgatactt
      181 taaaaagtat cttaaaagac aatcaagata aacctaataa tataagagta tactttgcag
      241 gtgttggttg tggaggtcct tcttttggat tagctttaga tgagaaaaaa gaagatgact
      301 taacttatga agttggagaa ttacaatttg taatgagctc tgatgaatat agtcagtatg
      361 gagatataat aatagaagat acaggatttg gatttagagt tattcctgaa aatatgaaag
      421 atcaaggtgg aggaggatgc tctggttgtt cagggtgcca ctaagattaa gtttttagta
      481 agtttagaac tctctttata gaaataaatt tctattttaa gagagttctt tttatttttt
      541 ggtgagataa tgtataataa aaatgtattt tactaatttt ttatatgtaa ggagaattat
      601 tgtgagaaga agacgaaaaa aaggtgcaga tgaaaagctt ttaagttata caaaatatgt
      661 tttaagagac gatatagaca aattaaaagg caagtggaat ttaaagttta gaaatgataa
      721 tccaatacat gttgaatttg ggactggaaa aggtaagttt ataacaactt tagctaagca
      781 gaaccctgac ataaattata ttgccatgga acttaaggaa gaagttcttt taaaggctgt
      841 tgaaaaagca gatgcttcta atttgaataa tatactattt ttatggggag atgtaagcaa
      901 tattttggat tactttgaag ctaaagaatt atcgagaatt tatataaatt tctgtgaccc
      961 atggcctaaa aatagatgga gtaaaagaag actcactcat tctggatttt tagaaatgta
     1021 caatagggtt ttggaagatg atggcgaaat tcactttaaa acagataatg aaaaattatt
     1081 cgagtttagt ttaaatgaaa ttgctgcaaa taattggcta ttaaagaata tttctcttga
     1141 tttaggaaat agtgagtatg aaaataatgt tacaacagag tatgaagaca aatttatgtc
     1201 acaaggtatg cgaatattta gatgtgaagc taaaaaacgt aactagtaat atgtttataa
     1261 gtttaatagt agaaatttat tttaatttat attaataaag tgtacgaaag taggtatata
     1321 taacaaggaa acagaaccct ctcatagaaa ttaaattatt ataataaaaa atttatgtgg
     1381 gagggtacta tgaatttagc taatttaata ccatttatta tttctgcagc agctttaagt
     1441 ccaataaatt caaatgataa ggttttgagc gaaaatcaac ttaaagatat ttttcctgat
     1501 gcaaatttaa gagcagttgt aaaaagatat ataaatccag atgagatgac tataagcaat
     1561 ataaaggctt tagatggaga attttatgca actggtgaga gcatttcaaa tttaaaaggt
     1621 atatcatatc ttgaaaatgt agacaatttt attttttgga ataataatat aaaagagatt
     1681 cctaaggaag ctttaagtct aaaagatatg gattcaataa atcttgccaa taattatttg
     1741 attgatgacg atgtagtaaa ttctctttct cataatggtg tggatgtaaa ttgcgattta
     1801 aattttattg atacaaaaga taatcagtat aaactggact ctaaatatca taacgttgat
     1861 atagcaaaag gtgaaagtat tgatttgaga aaaataatta cgaagaaaat tgatagttat
     1921 tataaatact gggaagttac tgataacctt ccaaaagatt taaattttat tgtatctgta
     1981 agtgataaga gtgtactttc atgtgaagat atgataatta aaggtaataa aacaggtcaa
     2041 gcagtggtaa aagttatact aagcgacaaa aatgatttaa atacatctca agaagttcta
     2101 attactgtca atgtaaaata atctgtcaac ttattcaatc attctatttt caatttaagt
     2161 gtataatcaa aataaaggtt gttagtaaca gaacgctaat attagtgctt tgttactagc
     2221 aacctttaaa ttttatagaa atagattatc taaaaaattc catttcttct gttagtagcc
     2281 catattccta ttaaaattat cagtgaatat attatataca aaactattct tatatattgg
     2341 acatctaatc caaaaaattc aatgttactc tgtgaggtat gcaacatcac aaggactgca
     2401 gatataccaa taagcaaagg attgattact atttgcttat cagttcttaa aatcattatt
     2461 ttacccatcc aaattattga taatataaaa aataaaattt taagaccaaa ttctaatgta
     2521 tctaccaaag cgaaaacatc ccttcattta taataaattt ttcagctcaa caaaatataa
     2581 aaccctacaa acaacttttg aatccataat aagataactt gattaagcac tatgaaataa
     2641 gtactataaa acttcatcta cattaatata tactacacta tacttcaaat ttcttaaaca
     2701 taaattaatg aaattaacaa aattttattc agaaacatat aaagatatat tttactattt
     2761 aaatgcatat cattatatat acagttttag gaggtgaggt atatggaatc taaatttttt
     2821 aattggtaca ctcaatcctt agggggaact tttggcatta tatcatgtat atgcgcatat
     2881 ttaaatggat acatgtttgt ttatacaaat agtgctagca aatattttga aagtatagga
     2941 tttctgggaa taatttcaag ttatacacta cttccacttt gtatgcttac tttgattttt
     3001 ggagttatat attcttatac tccagaaaca tccgacaaaa gtatttttaa tatacctttt
     3061 tttactttaa ataaaagatt aatacttatt acagtagttt ttggttttat aggtgctaga
     3121 gtttacttta taattcctgc aattctaata acttttaacc tatatagcaa tgttttgata
     3181 gctaaattaa agtacatcct atgtaaaaat aaagaaaatg atactaattt gaaagaaagc
     3241 gaagtctaaa ttgcataata ttggtatttg tcttaaaaat tcccgaggta ttaatttgta
     3301 ttctcttgta ttttgcaaaa aatattgaat tttattcatt ttatacttat aatttatatt
     3361 aataatggaa aattaaaaca aatataagtt actggttgtt aatttaaaat taaaagatat
     3421 attgatgtgt tgattctcta gtcagcactt tttcttttat aaatttgttt tttgtgatat
     3481 aatattagca ccaggtaata atacaagggg gtaacaaaat gaacatcaat gaaattaagg
     3541 aattattaaa ggccatagac tcaactaact tagaatatgt aaaacttgaa agtagcgatt
     3601 taagattaga agtatctaaa aaagctcaat ctacaagttc acctgtttta agtgtacaac
     3661 aagaatcagt agtggatctt tctttagaaa agccagttgt aaacgataca cctgtcacat
     3721 ctaatgagaa tctatcagta gtagtagctc ctttgatggg aacattctat gattctccaa
     3781 gtccagatgc agatagcttt gttaaagttg gcgatgtagt tgaagaaggc gacactttat
     3841 gtatattaga agcaatgaag cttatgaatg agataacaag tgaaattaaa ggtgaaataa
     3901 ttgaagtatt agttagtaat gaagaactag tagaatataa tcaaccttta tttaagataa
     3961 aaccattgta gtaggaggta tttaagttga taaaaaaaat actagttgca aatagaggcg
     4021 atatagcagt tagaatcatc agaacatgta aagaactagg tataaaaact gttgctatct
     4081 attctgaaat agataaagat tgttttcata gatatatagc tgatgaatct atttgtatag
     4141 gaccaaataa tataagtaag agttataata atatagaaaa tataatctat cttgctctta
     4201 aattaaagtg tgatgctatt catccaggct ttggattttt atcagagaat cctgaatttg
     4261 caaagcaatg tgaagataat aatattattt ttataggtcc aacaagagaa caaatgattt
     4321 tgatgggaga taaatctaga gcaagagaaa ctatgatgga attgaatatc cctgttgttc
     4381 ctggttcaga atctgttttg aaaactaagg aagaagcttt agaagttgca agagaaattg
     4441 gatatcctgt tatgataaaa gcctcaagtg gaggcggtgg aaaaggtatg agaatcgtta
     4501 gaaaagaaga agaacttttt tcaaattttg atatggcaag ttcagaagct ctagcagcat
     4561 tttctaatag tgatttatat atggaaaagt ttatagaaaa tcctagacat atagaagtac
     4621 aagtatttgg agataaacat tcaaatgcaa tacatttagg tgatagagat tgttctatgc
     4681 aaaggcgaaa tcaaaaagta atagaagaat ctttaagtcc atacctttca gatgaagaaa
     4741 gacaaaaact tcataaaatt gcagtagata tagtaaaagg agtaggttat atcggtgcag
     4801 gtactattga gtttatagtt gacaaagata aaaactttta ttttatagag atgaatacaa
     4861 gaatacaagt tgaacatcca gttacagaga tggtaacaaa tcttgacctt ataaaacttc
     4921 aaatatctat agctaatggg gataaaattc cttttaaaca agaagatatt acttttagag
     4981 gtcatgctat tgaatgtaga ataaatgcag aagattcatc aaagaatttt gctccatcac
     5041 caggtaagat agaatcatta aatttacctg gaggttttgg tgttagattt gatacttttg
     5101 tatatgcagg atatactata ccacctttat atgattctat gataggtaag ttaatatgtt
     5161 gggctgaaac tagagaagaa tgtataagta gaatatatag agctctagac gaaattattg
     5221 tagaaggtat taatacaaat gtagaatttc aaaaggcact tgtaacaagt gaggagttta
     5281 gagaagatac tcatcatacc aaatttatag aagatgtctt tatgaagaaa gaatttgcta
     5341 cattataagg agtaaataga ttatgataaa aaaattttta tcatcaaaag aatctaagta
     5401 tgttacgatt tctttagatg acaactttaa aaagaatagt gtagatgata aattttggac
     5461 ctattgcaaa ggttgtgata gtcatgtatt tagaaaggac atagaagaaa attcatttgt
     5521 atgtccaaaa tgttcaagac attatggctt aagagcaaga aaaagaataa atttattgat
     5581 tgataaagga acttttatgg agtttaattc tgatgtagaa tttcaaaatc cactaaattt
     5641 tcctaaatac aaagaaaaag tagactctta taaggaaaaa acaaaagagt cagaagctgt
     5701 tgtcacaggt tatggaagaa taaatggaat taaaacagtt atttgtgtaa tgaatcctga
     5761 ttttatgatg ggaagtatgg gttctattgt tggggaaaaa ataacttatt caatagagta
     5821 tgctgctgaa aataatttac ctataattat ttgttctgct tcaggaggag ctagaatgca
     5881 agaaggtatg gtatctctta tgcagatggc aaagacttct caagcacttt ctaagcttga
     5941 agaaaaatta ttgccatata tatcagttct aactgaccct actactggag gagtaactgc
     6001 tagttttgct atgcttggtg atattataat ttctgagcca aatacgttga taggttttgc
     6061 aggtccaaga gtaatagagc aaacaataaa tcaaaaactt cctgaaggat ttcaaacttc
     6121 tgagttttta cttgaaaaag gttttattga catgatagta gatagaagaa aaatgaagga
     6181 agtattatat caaattcttg ctatgcacaa gaaatagggc ataaaggagg aactgttttg
     6241 attgaaaata atagtgaaaa aataaaagtc ttagaaaatg atattaaaca attaataact
     6301 atttctaagg caaataatat agatttaagt gataaaataa attcacttaa tgagaaattg
     6361 gaaaagctta aggaagatgc tttttcacat ctatcagcat atgaaaaggt aacattaagt
     6421 agagatataa agagacctac gacattggaa tacatagaac atatttgttc taatttttta
     6481 gaacttcatg gagataggct ttttaaagat gacccttcta tggttggagg aattggtcag
     6541 ataggaaagt ttaatgttac tatagttgga caccaaaaag gaagagatac aaaagaaaat
     6601 ataaaaagaa attttggaat gcctcatcca gaaggataca gaaaagcatt aagacttatg
     6661 aaacaagctg aaaaatttaa tagacctata ataacgttta ttgatacatc aggagctttt
     6721 tgtggtctag aagcagaaga aagaggacaa ggagaagcta ttgcaagaaa cttacttgaa
     6781 atgagtaaac tttctgttcc agtaatcact tttgtaattg gtgaaggtgg tagtggagga
     6841 gctttaggta tcggagtagg aaatgatgta tgtatgttag aacattcagt ttattctgta
     6901 atttctccag aaggactttc tagtattcta tttaaagatt catctaaagc taaagaagct
     6961 tgtgatgtta tgaagcttac aagtaatgat ttatatgatt taaaaataat agataagatt
     7021 ataaaagaac ctcttggagg agcacaaaaa gatgtagaag ctgtttcaaa agaaataaaa
     7081 gcttatattt tagaaagatt aaatcattac aaagatatgg ataaagagga aattatagct
     7141 caaagatata aaaaatttag aaatattgga aaatgcttat agaatataaa gaaatttgta
     7201 gttttttcat aataaaatgg ttaaaaaaaa taaaggtttt cttaaaacga ttatagaagt
     7261 atatttttat aaaagtatac tttaaatgat gattgtaatt agtaaatggc aattatgatt
     7321 tagtacttta ggtaatggat actttaacaa attaacaagg agatgttgaa atggaggtac
     7381 taaaagtttc atcaaaatct aatcctaatt ctgtagctgg agccttggct ggagtactaa
     7441 gagaacgagg cgttgcagaa atacaagcta taggagctgg agctctaaat caagcgataa
     7501 aatcaattgc aatagcaaga ggttttgttg cacctagtgg aatggattta gtttgcattc
     7561 ccgcatttac tgatatagaa atcgaaggtg ataagaaaac agccattaaa cttataattg
     7621 aacctagata atatatctct ttattagaat ttaattaagt tttaaaggtt ggctatagaa
     7681 ataggacact atagtgaacc ttttttactt tatatgtttt attttatgta acttttaatg
     7741 tataatctta agtaaacaaa taagattgga ggaaatatac attatgaaat taaaattatt
     7801 ggtagaagag tatgcagtat gtagattaaa taatgacagt aaaataccaa catggataga
     7861 tacagagaag ttttactcta taactagaac tgatgatgaa ttaagtgttg tatgtttaaa
     7921 taataatatt ccttctgatg tcaagtcaga aaaggagtgg agaatactta agatactagg
     7981 tcctttagac ttttcacttg taggaatttt atctaagtta agtagtttgt tagcagacaa
     8041 caaaataagc atatttgcaa tttctactta tgatacagac tatatcttag taaaagaaaa
     8101 agatatagaa aatgcttgta aaattttaag ctgtaatgga tatgaagtag agtagttaaa
     8161 tacttttaca tgaggactta acttatgatt tatataatcg aaattttaaa aataagtgta
     8221 ataaatattt tttgtttcat aggaatattt attgtatttg gattgatttt tagcattaca
     8281 gaaaatctaa ataataaatt tatattttca tcttttggaa aaactggaat tattgtaact
     8341 ggagctatag gaacgtttgt tcatgaattg agtcatttga taatgtgttt aattttcatg
     8401 cataaaataa attcagttaa gtttttcaga ccaatagaaa gtaaaaatga tggtatacta
     8461 ggatatgtaa gtcatagcta taaaaaagat aatctttatc aaagtatagg aaatttcttt
     8521 ataggaattg cacctattat aggtggaaca ataatgatta ttttaatttt taagatatta
     8581 ctgccagata gttatatgaa agtgactcaa aatatagatt taaagttata tgtaagtatg
     8641 attaataatt taaatataaa tgggtttata cacattattt taggtgatat ttccaatttt
     8701 ataagaataa tgttgttaac acctagtgta tattcattga agtattttat atttatgttt
     8761 ttaatgtatt ctatatctac acatatgtca cttagtagtg ctgatttaaa aaatagctta
     8821 aatggtctga gctttatagt tatagttatt tttgtaataa gttcaattac atacttattt
     8881 ggatttgata acttgaatat atccagttta atagttaagt ataacatatt tataagtttc
     8941 tttatggcaa tagggtttat tttttcaata gtaactctca taatatccta tattttaagc
     9001 ttagtcagcc catttaaaaa ctttagttaa ataaatacat attttactaa tatttggttt
     9061 cgttagaaac atataaaaat attatataaa aaagatttta gtagagcata aaaaatttct
     9121 caatatattt taaaatattt ctataaatat attgagaaat aaaaaagagt acttaaattc
     9181 aactagtctt taaatgtgga tttagggaat agttagtgat aatttcatct aaagcactgc
     9241 taatctctta aaaatgtaaa aaatgattaa attaaatatt tatactcact tggtataaat
     9301 atcaaaaaat aatataaaag atttatattt tagttttagg aggtttaatt ttgaataatg
     9361 ataagaaaat tataaaagta cataataaat cagataaacc tgataatatt gttatcaaag
     9421 aaaatgaa
//
LOCUS       DBGJFU010000142         9360 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.143, whole genome shotgun sequence.
ACCESSION   DBGJFU010000142 DBGJFU010000000
VERSION     DBGJFU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.143"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            190..837
                     /locus_tag="ACOIAS_003206"
     CDS             190..837
                     /locus_tag="ACOIAS_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HGZ2702896.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1004..1573)
                     /locus_tag="ACOIAS_003207"
     CDS             complement(1004..1573)
                     /locus_tag="ACOIAS_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGZ2702897.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1578..2477)
                     /locus_tag="ACOIAS_003208"
     CDS             complement(1578..2477)
                     /locus_tag="ACOIAS_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2702898.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGILLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2488..2637)
                     /locus_tag="ACOIAS_003209"
     CDS             complement(2488..2637)
                     /locus_tag="ACOIAS_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HGZ2702899.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2630..3214)
                     /locus_tag="ACOIAS_003210"
     CDS             complement(2630..3214)
                     /locus_tag="ACOIAS_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HGZ2702900.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            3603..4928
                     /gene="cdeA"
                     /locus_tag="ACOIAS_003211"
     CDS             3603..4928
                     /gene="cdeA"
                     /locus_tag="ACOIAS_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HGZ2702901.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFISSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(5023..5817)
                     /locus_tag="ACOIAS_003212"
     CDS             complement(5023..5817)
                     /locus_tag="ACOIAS_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2702902.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(5818..6693)
                     /locus_tag="ACOIAS_003213"
     CDS             complement(5818..6693)
                     /locus_tag="ACOIAS_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2702903.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(6697..7065)
                     /locus_tag="ACOIAS_003214"
     CDS             complement(6697..7065)
                     /locus_tag="ACOIAS_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2702904.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(7248..7907)
                     /gene="deoC"
                     /locus_tag="ACOIAS_003215"
     CDS             complement(7248..7907)
                     /gene="deoC"
                     /locus_tag="ACOIAS_003215"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGZ2702905.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(7943..9130)
                     /gene="murJ"
                     /locus_tag="ACOIAS_003216"
     CDS             complement(7943..9130)
                     /gene="murJ"
                     /locus_tag="ACOIAS_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGZ2702906.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(9207..>9360)
                     /locus_tag="ACOIAS_003217"
     CDS             complement(9207..>9360)
                     /locus_tag="ACOIAS_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGZ2702907.1"
                     /translation="NKCGYKNEEVKNLNIREWICPSCNETHDRDINASRNILKEGLRL
                     ITIQNK"
BASE COUNT         3181 a         1506 c         1030 g         3643 t
ORIGIN      
        1 gttatttatt aaaaactaat agtaaatctt atactaatcg catatttatg ttataactat
       61 attgtaacta ttttcgtaat cttaggaaaa gattacttat aaaattaacc ttatacaata
      121 atatatcgta taatcttaat tagcataata gttacaatat taaaatttaa atatgggagt
      181 gattttagta tgataagaca aacaccatta gagaaacgat atgacaagct aggtcctcat
      241 attgtaacag cactcaaaaa acgttatttt gatgcttatt actgtaaaac tagaaatgaa
      301 gctttgcaac aaattttaga tttaattcct caaaatcatg tagtatcatg gggaggatct
      361 gaaacactta aagaaatggg cgtacaaact gctgttagag agaaaggatt taaagttatt
      421 gatagagatt tagcaaaaac tccagaagaa agaactgaaa ttatgcgtca agcacttcta
      481 tgcgatactt ttttgatgag tagcaatgca attagtgaag atggacaact ctttaatatt
      541 gatggaaatg gcaatcgtgt agctgcaatg atatttggtc caaaaaatgt tatagttgta
      601 gctggtatga ataaaattgt aaaaaacatt gatgatgcaa tacaaagaac tagaacaatt
      661 gcatctccag cagttgttca gcgttttcaa gatgtcaata caccatgcta tattaatgga
      721 agttgtatag attgcacaag ccctgaaagc atttgtgcat acatggttac tacaagaatc
      781 agtcgtccag caaaaaaaat aaaggtaata ttggtaggag aaaatcttgg tctataaaat
      841 atacatacat gtaaatagct ctataaataa taaaatcata tgaaataact tatttctaaa
      901 agtggtagaa taaaagcttc tacaagactt tcattctacc acttttatat tcttttaatt
      961 tgtgtttcta tattattaat tgctccgatt ttcatatata ttactaacta tttttatttt
     1021 cataattttt ttgttcttca tcaagctgtt tataataatc tttattatag tcagctagat
     1081 aatctatacc acctcctgta atcacattta ataatttcat tgcagattct tgattaactt
     1141 tacaaacata attatcacca gatttaacaa gctctaatgt aatattaaat tcttttttct
     1201 ttgcagaatc aatatctttc atagttggat ttggcatcat ttctcctagt ttaggaggat
     1261 tcttttttaa aatagaaacc atatcagtat tgttaatttt taaatctaca gtcactttat
     1321 ccttattatt ctttatatct gaaatttccc aagtcatatt tttagaatac attttaaatc
     1381 cttctttatc ttcgtatata agattaccta accctgattt tgggaaatac ttatcaacag
     1441 tatctgcatc ttgtgttttt attgaattaa acatactatc cacttcattt tttatcttat
     1501 tatcattagt acaaccagct aacaccagta ctgatattgc aaacattata atacatataa
     1561 taaatttctt cattaaatta tatcccccta tacttttttg cacaaatatt attctctttt
     1621 ttatcttttg aaaaagttgt agtaattgca ttttgtggac aaacttttat acattcacca
     1681 cacctaatac agtctggact atttacatct ttatatattg gtatatcaag cttacatttt
     1741 gtttgacatg ctgtacaatt tgtacattta tctttatcaa ttttaaatct atatattgaa
     1801 ataggattaa aaaaaccata gattgctcct aaagggcata tatacttgca aaatggccta
     1861 taagtaatga ttgacatcaa taatatcaat agcaaaatac ttaacttcca gctaaataaa
     1921 aatcctattg attctcttaa attctcattt cctaaaataa gtagtattcc tcccaaaagt
     1981 gttcctgaag ggcaaatcca tttacaaaac caaggatttc caccccctgc tacatttaca
     2041 atagcaactg gcaaaataat tacaaacatt attaagacta tatactttag atattttaga
     2101 tatttatcac cataaacttt ttttatcttt actggaaagg gtactttatg tagtaaatct
     2161 tgtacaagtc caaagggaca aagccaacca caaacaaatc tgccaaaaaa agcaccaaat
     2221 gccattaaaa aaccaacaac ataaaatgag aatttatact ctctgcttga taaaactgct
     2281 tgtaatgaac ctattggaca tgctccaagt gcacctggac atgaataaca attcaatcca
     2341 ggtacacata attttttact ttctcctcca tatatagttc cttctaaaaa tccatttaca
     2401 tatccatttg ttaatgctgt aaatgcaact tgagtaataa gtcttatatt attatttacc
     2461 ttagataata acttcatccc tcttctccta accaatacca atacactcaa gacaaatcat
     2521 aattgcttta gttaatactg tatttgtttc tccacgatat gtacctaatg caataaatat
     2581 aatactaatt gcaataatag aaatgccttt tttattatct ttaataaagt tactcattgg
     2641 cttttaccat ttttagtctt tcatctataa tttttttcca atcttcctta ctatttgcac
     2701 ctacaacctt ctcacccaca aagtttcctt tcttgtctac aaaataagtt gttggaaact
     2761 ccataactct tttaaaatca gtttttttaa gttcttcaga tacagtcaat tgttggtaag
     2821 ttgcatctga ctttttcata atgtctttaa ctaaattttt ctcttcattt gaaagaccag
     2881 ttctcatatc agttttatca acttctacaa ctaatccctt tatagctaca ttggaattat
     2941 tactctcata ttcttttgat aatgcttcaa gctctggcat ttcacccaca catggtccac
     3001 accaagtagt ccagacatta ataagagtaa gttctttatt tgaaaatatc tcttttgtta
     3061 ccttatttcc atcaacatcc tccgtatcta aattactaaa ccattcacta ttattcttgt
     3121 ttttttctac tggcttagat tcttttttgc tacatccaga aatcatacct aaaacacaaa
     3181 tacatcctaa aactactatg aaaaaccttt tcatatctca cactcctaga atttatatag
     3241 tatataaaat cgctgcttga aataaaaata acttatcttc tatattaaag taattatatt
     3301 taataaataa gagctaattt aaaaaaagat atacagttaa aatctatttc ttttcagaca
     3361 aaatcttata tatatataat agcttgtaaa tcttaagatt atatacatat atttctaaag
     3421 attttcttaa gaaacagtta gattttacta atataagttt attaattagc attatactaa
     3481 tttatcttaa tatgattttt tgttgatttt aagctgttta tcgtataaat aaaatttttc
     3541 ttgactatat agttactata tagcttatta tatatagaga gtaataaaag gaggtaaaat
     3601 ttatggaaaa tttatttaca agaaaattca ctacttttga atttctaaaa tttgtttctc
     3661 ctgcaattat atccatgata tttatatctt tgtatacaat aatagatggc atctttgtat
     3721 cgacattagt tggttctgat gctcttgcta gtataaatat tgtactacct ataattaacc
     3781 ttgtttgtgg atttggaata atgatggcaa ctggtggagg tgctatcgtt tctatacgta
     3841 tgggtgaaaa tagacaggat gaagccaact ctacattttc ttttatagtt ttgttttcat
     3901 tgattgttgg gattttattc acagtaatct catatttctt tatcaaagaa atatctattt
     3961 tgcttggtgc aacagataag ttattaccat attgtataac ttatggtaaa gttatgattt
     4021 tatgtacacc attttatatt ttaaaattta tatttgagta ctttgcaaga actgatggaa
     4081 attctaaatt tagtttattt ctatcagtca ttggtggtgt aacaaatata atattggatt
     4141 atgtatttat taaatatttt ggaatgggtc ttttaggagc tgcagttgca actgctatag
     4201 gtattatttt aacttgtgtt ttaggtatta tttacttctt atctaataaa tctacactaa
     4261 aattaagaaa accaaaaacc gattttagac ttataagaga tactatgata aacggttctt
     4321 ctgaaatggt tacagaatta tctacaggaa ttacaacatt cttatttaat gtagtagctt
     4381 taaaattagc aggagaaaat ggacttgctg ctcttactat agtattgtat gctcattttt
     4441 taatgacatc agtctatcta ggattcgctg ctggagtgtc tccattaata agctataatt
     4501 ttggtgctga aaacagtgat aaattaaaag aaacatttaa acattctcta aaatttatat
     4561 ttatttcttc tcttttagtg tttattattg ctttagtatt tgcaccattt attgttaggg
     4621 tctttgtaaa tccagataac acagtattta aactagcctt acaaggatta aaaatatttg
     4681 catttgcttt tttgtttgtt ggtataaata tatttgcatc aggatttttt acagcatttc
     4741 acaatggaaa aatttcagct attatatctt ttagtcgtgc ctttgttttt ataatcatag
     4801 gaatcataat tcttcctcct atgttaaaca tgactggatt atggcttaca gttccatttg
     4861 ctgaggttat aaccatattt atatctattc tatttataaa aaaatataaa tgtagatata
     4921 agtattaaaa attaatactt aaaatcaaca tttattaaaa ctaataaacc tgctttaaaa
     4981 tacaaattta caaatttgat ttttaagcag gttttatttt agttataata agtcatttgc
     5041 atactcttta attggtgctt ttctaagtaa taaaattata aatattatgc aaataactat
     5101 aattcctacc tttgttccaa ataaaataaa aggattctga aatgaataaa taattttttc
     5161 taaatacaat agcattttat gcatattttt agttgcaaaa ttaactatat aaattaccat
     5221 taatatcggt aatcctatca atatagtaat actagtaact tttctaactc tatatgtaaa
     5281 agctccaagt aacactccta taaaacctat acatatttca tttatacata tttgtacaaa
     5341 ataatttaaa taagttagtg gcatacttga tgttgaagga tctatcagtg ctgatataga
     5401 tgtccaaatc atatctctct ttagtaaaac tgatagttct atactgtaac ctgttataat
     5461 ttctataaat aacttcgata aaaaaatcaa aactatacct aaaatagaaa tcacaaagct
     5521 taaaataatt aaatctatga caatagcttt tatacaactt tttctatctg ctcttatact
     5581 taaagctcct gaaaagtctt tatttgctat atttactcca tacacaaata gcagtatagt
     5641 tatatatgtt aaaataggat tcacaatcgt ggataaatta gttttagatg cttctccaaa
     5701 aatcttgaga ataaacactg gcaaaatatt cataacaatt cccacaatca taaatataat
     5761 tatatacaat ttagtatttt tattaaaaaa ttttatatat ggctttaatt cattcaatta
     5821 caattcctcc ttcttagtca tgttaacaaa catatcttgt agccctatgt atcctacatc
     5881 aatatttgaa tttttaagta ttttttcatc attttcacta aaatcaccat agtaaaaata
     5941 agcaactgca tttccaaatg cctttttagg ttttatattt tttatacatt ctaatttttc
     6001 taactcttct ttatttccag taatataatg agctttttgt tttatgacat caatttcttc
     6061 atctataata atttttccct catttaatat tattacatgc tccagtaaat catctatttc
     6121 atcaatcaaa tgagttgata ttattattgt ccttggattt tttatataac tatctaaaat
     6181 tatgttataa aactcttgtc tatttactgc atccaaacca attgttggct catcaaatat
     6241 ggtgatagct gaattagaac aaatacctat tatatttgaa agtatagttt tcatacctct
     6301 tgacagttgt ttatactttt ttttagtatt taaatcaaaa tatttgcata attctttttc
     6361 taagccttta tcataacttt tataaaaact agaagaaaat tcaaatatct gacctacttt
     6421 aaaatctgga ctataaaatt ctttttccct gacaatacaa acttcttcca aaacttttat
     6481 atcttctttt gggttctttc ctaatatttc tatctcacct tcattttgaa ttaactgatt
     6541 taccattata tttaataatg ttgtttttcc agctccattc ttacctaata aaccatatat
     6601 cttatttttt tcaaagttta gagacatatt attaaaaata ttttgcttct taaaactgtg
     6661 agataaattt tctatcttaa tagaactact catatattaa tcctccttga aattaattat
     6721 aatatcaacc aattcttctc tatttatttc taatttgtta gcttcttgaa gtaggttttt
     6781 tacaaaatta tgtttaaaat tttcttttct agcttctttt ataatagttt ttgcaccatc
     6841 tgatacaaac atccctatcc ccctcttctt atataaaata tttttatcaa ccaatatatt
     6901 tatacctttt aatactgtag ctggattaat tttataaagc tttgaaagct ctgttgttga
     6961 tggaacctgt tcttcttctt ttattccatt tgatagtatc tcatcttcaa ttgctcgtgc
     7021 tatctgaata aatataggtt cttcattatt taatactaat ttcataggtt cctcctttct
     7081 ccattagtta gttactcaac taattaacta tataacttat aatatcttga aaaatatttt
     7141 ctgtcaatac taaataaaaa acagagggaa aattccctct gttttttgtt ctttatattt
     7201 ataattaaat tttgattttt gtaatcctat cccataagtt aaaaacttta ataatctgtt
     7261 gtatcttcat tttttaatat tttacatatt gagctagttc ctaacctttc tgctcctgcc
     7321 tctataaatt tttctgcatc agaaattgat ttaactcccc ctgcagcttt tatttttaca
     7381 ttttttccaa catgctcttt cataagcttt atatcttcta aagtagctcc acctgttccc
     7441 attccagtag atgtttttat aaaatctgaa cctgacattg ttactatttc acacattttt
     7501 attttttcat cttcatctaa ataacatgtc tctataatga cttttaatac tttatctcct
     7561 atagcttttt ttatttcttt tatttctttt cctatattat cataatcttt attttttata
     7621 tctgaaatgt ttacaaccat atcaacttca tctgctccat tttcaatagc atcttttgct
     7681 tcaaatactt ttgtagcagt agtttgatat cccaaaggga accctatcac tgtacatatc
     7741 tttattttac cttttagata ttctgatgct cttttcacat atgatggtgg tatgcaaact
     7801 gatgctacac tcatatctac agcttcatca cataaaattt taatatcttc ccatgtagtt
     7861 gtagctttta aaatagtatg gtctactgtt tttaatatat gtttcataaa atcctcctaa
     7921 atttataatt aatataatta gattactttt ttaaatgcaa ttttctcttt gcaatattca
     7981 gtatcatatc aacctcttca actttaaaaa tagtcattag tagtgtataa attccacctc
     8041 caactataac tgaaatagct agtgagacaa actcattaaa tgttcctact cccaaaattc
     8101 caaatataaa tttatacgta aagtatgata aaacacccat aattaaagat gcaactaccg
     8161 atttcaaagt tgcttttatt attttatctt gtccaaaata tccaactttt ctctttaggt
     8221 tcaagaagag taacaatata catgcaattg atgatgaact agttgcaaga gcaagaccac
     8281 catgagccat tggttttatt aaaactaagt ccaatactat atttacccct acagatataa
     8341 tcccatttac cataggtgtt tttgtgtctt gtaatgaata aaaaaccttt cccaaaatat
     8401 ctcttaagcc aaatgcagtc ataccaactg cataaaaaat taaggcagtt gctgtcattt
     8461 gggttgccct cgcatcaaaa gcacctctct caaaaatgat tctaacaaca ggagttgcaa
     8521 aaaaaataga agcaactgaa ataggaatca tagaaattat tatcatattt atactagatt
     8581 taactgaact agtaaacttc ttttgattat tttctgcact taacttagat aacataggat
     8641 acataacaga tactattgaa gctgtaaatg tccctgtaac aaaaccattt aatctatctg
     8701 cataagtaag tgctggaatg cttcctttaa ctaatgttga ggctaaagtt gtatctacca
     8761 atgagttcac ttgattaaca gcaacaccta taaatactgg tgaaagtaat gctagtaatt
     8821 taataaggct atcatcttta aagtttaagt agtataagta tttgtagttc gtttttttaa
     8881 caaaaaacat gtaaaagagc agttgaacta ccatagcaac cacagcacca attggaagta
     8941 tgtatggtcc aaatatggta cttaacatta ttgaaataat tattactata ttatagggta
     9001 tacttccaaa tccaactaca ataaagttct ctttaatttg taaaaatgct gacataacac
     9061 ttgtaatacc tatgaatatt attcctgttc tgaacctccc atgactaaag tcacagggtt
     9121 cctgcttcat agaattttgc atactaaaat atgtcttact aattctccac aggctatccc
     9181 cgtagttcct acggttctta catacgttat ttattttgaa ttgttattag tcttaatcct
     9241 tcttttaata tatttctact tgcatttata tctctatcgt gagtttcatt acaactagga
     9301 caaatccatt ctcttatatt taagttctta acttcctcgt ttttataccc acatttattg
//
LOCUS       DBGJFU010000143         9213 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.144, whole genome shotgun sequence.
ACCESSION   DBGJFU010000143 DBGJFU010000000
VERSION     DBGJFU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.144"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(56..628)
                     /locus_tag="ACOIAS_003218"
     CDS             complement(56..628)
                     /locus_tag="ACOIAS_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGZ2702908.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(657..923)
                     /locus_tag="ACOIAS_003219"
     CDS             complement(657..923)
                     /locus_tag="ACOIAS_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HGZ2702909.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(923..1132)
                     /locus_tag="ACOIAS_003220"
     CDS             complement(923..1132)
                     /locus_tag="ACOIAS_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HGZ2702910.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1401..2261
                     /locus_tag="ACOIAS_003221"
     CDS             1401..2261
                     /locus_tag="ACOIAS_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGZ2702911.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2309..3337
                     /locus_tag="ACOIAS_003222"
     CDS             2309..3337
                     /locus_tag="ACOIAS_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HGZ2702912.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            3708..4214
                     /locus_tag="ACOIAS_003223"
     CDS             3708..4214
                     /locus_tag="ACOIAS_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HGZ2702913.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            4627..5295
                     /locus_tag="ACOIAS_003224"
     CDS             4627..5295
                     /locus_tag="ACOIAS_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGZ2702914.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            5454..5960
                     /locus_tag="ACOIAS_003225"
     CDS             5454..5960
                     /locus_tag="ACOIAS_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGZ2702915.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            6088..7104
                     /locus_tag="ACOIAS_003226"
     CDS             6088..7104
                     /locus_tag="ACOIAS_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HGZ2702916.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     regulatory      7300..7394
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            7458..8162
                     /locus_tag="ACOIAS_003227"
     CDS             7458..8162
                     /locus_tag="ACOIAS_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HGZ2702917.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            8457..8936
                     /locus_tag="ACOIAS_003228"
     CDS             8457..8936
                     /locus_tag="ACOIAS_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HGZ2702918.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         3756 a          870 c         1491 g         3096 t
ORIGIN      
        1 aacacaacag taaattataa attatcaaat aataatagtt atacaaataa aaattttaga
       61 acattttttg agattcttct aactcgtaag catccattaa ggcttcacca aatctttgat
      121 aatgaattat ttctctttga cccaagaatt ttaatacatc tataacatca acgtcatctg
      181 ttaggtttat aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat
      241 gtaaatcggt tacgggattt gacataactg ctataggtct tacatcaaat ggaacaccat
      301 ttgaatctgt tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag
      361 cttttaactc ttctgggctt gcatcagaag ttaactgata aaccatagta catataagct
      421 caacgtgagc tagttcttct gtaccaatat ctgttaaaag tgcacgcata ttaccagtag
      481 gcattgtata tctttggctt aaatatctta aagatgcagc aagttctcca tttggcccac
      541 caaattgagt tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt
      601 gttgtatagt tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa
      661 taattaaatt ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt
      721 ggagcatacc caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc
      781 gcaaattgat tacataatga attataagtg ttgatagcat ttttatcatc agggtgattg
      841 tctaaatata agttcatatc aacacatgca aaagatgttt cctgaacttt tctcataagt
      901 tcagctctag atgagtcttt cattatcttc tacctcctct atattttttt ggcttagcat
      961 ataattcttt gttgtacatt tcagactcta gtaagtttag ttctggaaat atagttccaa
     1021 attttaatgc atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc
     1081 tagccaaaga tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa
     1141 cctccttgtt gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa
     1201 atcgttacta ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg
     1261 ttatttatgt tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta
     1321 aagtaaaaat tatagtattt tgtggatgaa ggtagacttt tttgtaaaat attattaaca
     1381 taaatatacg aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc
     1441 cttttgagga aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagga
     1501 aaaatttatg gtctaagttt atgaaggcaa taagagatta taaattggtt gaagaaggtg
     1561 ataagatagc tgtagcgatt tctggtggaa aagacagtat tcttatggct aaaatgtttc
     1621 aagagttaaa aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg
     1681 gatatcatgc aaatataaga caattactta tagataattg tgagtatcta aatataccaa
     1741 tacatttatt tgattcaagg atatttgaga tagcagatga aatagcaaaa gattatccat
     1801 gctatatgtg tgcaagaatg aggagaggag cattatattc aaaggcagaa gaattagggt
     1861 gtaacaaact tgcattaggt catcattatg acgatgtaat agagacaaca atgcttaatt
     1921 tattgtgtgc tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag
     1981 gtataaaaat tataagacca ctttattata taagagaaga acatattata agatttattc
     2041 aaaatagtgg tatatggcca ttaaattgtg catgtatggt tgctgctaaa aaaacaggta
     2101 ataaaagata tgagataaaa gacttaataa agagcttaga atcaaatttt aaaaatgtag
     2161 aaaaatctat atttaaggcg gcagaaaatg taaatcttga ttcagtactt ggttggcaaa
     2221 aagatgggga gaaacattct ttcttagaaa attttgaata aaaatagtta aaaagaatca
     2281 ctataaacaa ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa
     2341 tatgccaaat tctaaatcaa aaagagcagt atttaattct gatataattg caatcggagc
     2401 agttatgtat gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt
     2461 aagtaatgag gagttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt
     2521 gaagagtgca ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt
     2581 ttctgatatt aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac
     2641 agatatgaga agtgctaaaa aaaataacca tccaaggttt gtaaataaca ttagagattt
     2701 atttgtagat ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc
     2761 attgaaaaat ttacttgaat ttgttaactt agagtttagt ggagatgccc atgacccatt
     2821 aaatgatgca tataatttat ttattttgga tgaggcaatc acaaataatg tagatataca
     2881 aaatctttta ataatacgtg acataataag accaccattt aacgatataa attctaattt
     2941 agataattgt tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat
     3001 tgtagatttt tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga
     3061 tgtaaatata caaaatttag aaattatgaa agatataagt aaaaaaatgg atactataga
     3121 taaattgaaa gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgatat
     3181 gaaagatgta ctagaagatt taatgcttta tagaatgaat gaagatgagt acaagtatga
     3241 gataaaaaat ataataaaaa tgtttgatga agacttagat aatgaaaaaa tatatataaa
     3301 taaatgtaat aacttaaatg ttgttaataa gatataagaa attataaata atagagtaga
     3361 acttgacaat tataacaatt ttttataaaa ctgaatcaag ataaaattaa attaatataa
     3421 aaataatata caataaaaaa tgtagagaga tacttacttt ctacattttt ttattgtata
     3481 ttgctttagg tttaaaaata tattatatac tttataatca atatatttta tctatatttt
     3541 acacttataa aatatgaaaa ctacttattg cgaaagtaat tgatttgttg tattataatt
     3601 tacatgaatt atgtaggaca atcaaaagtc agtattataa gagtacatat attcaaattt
     3661 ttaacaatta atttatataa ttaaaaaatg aagggtagga tgaagtcatg ggttttagag
     3721 aagtaaaaat tgaggaatta cagtttaatc catttactaa gattggcaaa gaatggttac
     3781 ttataacagc tggtgattca gaaaagttta acacaatgac tgctagctgg ggtggtgttg
     3841 gagtctattg gggtaaaaat gttgttacaa cttacataag accacagaga tatacaaaag
     3901 aatttgtaga tagtaatgat acatttacaa ttgccttctt tgatgaaacg tatagagaag
     3961 ctttaaatat ttgtggcact atttctggta gagatataaa taaaatagaa aaagcaggac
     4021 ttacaccata ttttgtggat gatacggtag cttttgaaga agctaatatg attatagtat
     4081 gtaaaaagct atatcatggt aatatgccac cagaaaactt tgatgctaaa gaaaatgata
     4141 aaaaatggta tccagagaaa gactatcata ctatgtacat ttcagaaatt ataaaagttc
     4201 ttgtaaaaga ataattataa taaaaaatat atgaaaagaa cttattataa gtaaatgact
     4261 gtgctttctt atctgatatc taagtagatg ctactaacta aataaatatt aattttaaat
     4321 aaatactatt ataaaaaaga tgaactatat ttactatagt tcatcttttt atttatataa
     4381 gcacttatat aagtagagat ttatttgaat aaaagtattt atttgaaata tttgagtata
     4441 ctttataagt atttttatgc taactagaaa cacttaattt atggcatttg tacttgattt
     4501 attttaatgt aaataatttc aaagttaatt ttattcaata tagtactata aaagtataaa
     4561 taacatttaa taaaaatata tattatatta ggtattttaa ataaattttt taaagggtga
     4621 ttttttatgg aaaatataac tagttatttt acaacaatac tatgtgtatt tatatgctta
     4681 tcatctgtat ttatatttac tcaactagct agagtattta ttaataagaa gaagattaat
     4741 caaaaaatta aatcaagaaa cggatttagg tatgatagag attttattga agccagaaga
     4801 gaagaaatac atataaagga taacaataat aataaaaata agagtaacaa taagaaatta
     4861 aaggaagaaa aagtatttaa atacgataat ggtgatttat ataaaggtga gtttgttgat
     4921 ggaaagaaaa atggatttgg aatatatatt ttttcaggta aggaaaagta tgaaggatta
     4981 tggaaagatg acaagatgca tggaatagga aaatatactt atagagatgg ttcaatatat
     5041 acaggtgaat ttaaatacgg attaaaaaat ggtttgggaa aattaacata tcccaataat
     5101 gacatttata agggatattt tttagataat aagaaaaatg gtaagggtgt attatataaa
     5161 aatgatggaa ataaacaagc aggaatatgg gaagatgatg aacaatgtaa aagtttagat
     5221 tttaaagatt taaataataa taaaataaat aatgtttatc aaaatagatt aaataacaat
     5281 aaattaaaag tttaattatt ctaataaaaa aatctttatt tatgatggaa tataggatat
     5341 atctatgttg taatttgtaa aatcttccta tatcatataa aattaagtat tgattagtct
     5401 attacataaa tatagaatat aaatatatcc atttaataat aaggagaaga gatatgaaag
     5461 agtatgtagt tgtggattta gaaactacag gattagatcc atataaaggt tgtgaaataa
     5521 tagaaatagg aataacagaa ataaaaaatg aacaaattgt aaaaaactat tcaaggttga
     5581 taaaacctaa ggggataatt tcttcattta taacagaatt gacacatata agtaatgaaa
     5641 tggtagaaaa tgaagaacca ctagaattag ttttacctag atttagaaaa tacataggtg
     5701 atagaactat aattgcacac aatgcgaaat ttgatttaaa atttttaaat tactatctta
     5761 gaatgttaga tttagaacca ataaacaatt atatatgtac agtagagtta ttaaaaaaat
     5821 gtaaaagtta taaaggaaaa aacaagaaac ttgaaacagc ttgtgcttat tacaacatag
     5881 agaatataaa tgctcataga gctgacagtg atacattagc aactgcaaaa ctatttttga
     5941 aaataaaaga tgaatattaa ttataaatat agagaaaatt agtgaaaata tcctttataa
     6001 aattgaccct taagcttaca attttgttct ttgatttatt ttgtagaaca atgtataatg
     6061 taccttatat gaagaaggga gactaaaatg ggttattaca atgataatga taataataaa
     6121 ataagacgta aaaaaattag taacaacaaa gatagtaata aaaaatctaa acattcttat
     6181 acttcggcaa ataaaaaaaa taaaagtcct aatacaagtt ccagtaaaag aatgagtgaa
     6241 aaagtagatt cacagaatct tataaaaaga gatagagata gttttacaag agataatgta
     6301 agacctttaa atacagatta caatcaaaga gaaagaagaa gagtcgagta taaacaaaga
     6361 gagaaaaaat taaaaagtaa aaaaagaaaa aaacaaaaaa ggattagaat agttagggca
     6421 ttattatcta ttatgttagt tgcactaatt atattttcag tggcaggtgc aatatttaca
     6481 gtttcagcta taaaagggtc accagaagtt actaaaaagt taattaggtc taaatatata
     6541 agtagtgagg ttgtaagtat aaaacaaatg ccaaatgatt taaaacatgc aatagtttcc
     6601 atagaagatg agagatttta taagcataaa ggagtagatg ttatttctct agttaggtca
     6661 gtattaaaca atgtgtttac agatacaaca caaggtgcaa gtactataga tatgcaagtt
     6721 tcaaaaaacc tgcttactag taatgataaa actatgaagc ggaaaataag ggatatgtat
     6781 aatgctaagc agatgaataa gattatgact aaggatgaaa ttcttgaagc atatttaaat
     6841 aatatgtatc taggtaaaaa tgcatatgga gctgcaaagg gagctgaagt ctactttggt
     6901 aaaaaagtta gtgaattaaa tttagctgag tgtgctatgt tagctggtat gacaaataat
     6961 cctgcaaggt atattgacca tggagaggct aagaagagac aagtgattgt actgtataag
     7021 atgcatcagc ttggttatat aactgataag gagtatagaa tagcaaaagc tgatgacact
     7081 ccatttaaat cagaaattga ttaaaattgt aagaatatag tattttcaat agtttaaaca
     7141 aacaaaaatg taataaaaaa ggtgatttgc cacccatttg ccaccataat tataatctaa
     7201 tggcaaattg ttttaatgta caaactagat aatagaatat tgtcttaaat gctatattat
     7261 gctaaaattt aattgtattt tgtaaattaa aaaacttata atttagtttt ctagggttcc
     7321 gcattttata atggtcaggt ccaagagaaa acgcgcaatt gttgtgtaca cggaaggata
     7381 aaagcctggg agatatttaa gaatatttct ataggctttt ttatttttac atattttaaa
     7441 tttgaggagg ttttctaatg aaaaaatttg ttattgagga tgcattttgg aatctatttc
     7501 ctagtgcaaa aattggagtt gttatttgta atgatataga taactctata aaagatgaag
     7561 actataagaa aataattttg aagggagaag aagaagcatt aaaatacttg gaggactcag
     7621 aatttagcaa caaccaagtg attaaagtat ggagagaagc atttaaaaaa tttaaaacga
     7681 aaaaaggagc aaggtcttct attgaagcat tattaaaaag agttcataat ggaaataata
     7741 taggaactat taacccttta gtggatatct acaattttat ctcattaaaa tatgcattac
     7801 cttgtggagg ggaggatata gataaattta taggagatat taggctaact agagcaattg
     7861 gtaatgagga gtttatccca ctagggacag atgaaaattc atttccatat gaaggagaaa
     7921 taatttataa agatgatggt ggagcaattt gtagatgttg gaattggcgt gaagcagtga
     7981 gaacaatgct tactgaagat actaacaatg cctttttatg cattgaatta gtagatgaaa
     8041 gtagatttaa agaatttgaa aatgctttaa atgaattagc taaaatagtt caagaaaaat
     8101 tgggtgggaa ttgtaaaatt tcaattttag atattgataa taaagaggta tcagtggatt
     8161 aatatggagg tgctgttgta atggactatt ctagttataa agatacttat atttagggta
     8221 agaaggtcta ttttagatat aacgattatc aaaggatata atatagaaaa taaaaatgta
     8281 gaatataaaa agcctttaaa ataagaaaaa aatgtatttt aaaggctttt attatactct
     8341 tttttatttg cataattaac aataaaaata tattgttaat tataatattt ttatttaaaa
     8401 acgatataat ataatgtatt atactatata taaaatattt taaaggagtt ggcatgatgt
     8461 ctaataaact tataaaaaat ttactaagtg gtatattaca aattttattt tttcttttag
     8521 gattagtaat tgttgtagga ggatttaaaa gttttatgta cctctgtttt agtggagaag
     8581 caactctaca aggtactatt tatggaatat taatgtttat tttaggtgta tcatatttta
     8641 tcattataaa atcattgata gaagtattag gtagttctga atatagccta tttgtaaaag
     8701 agaatgtaaa aagatttagg attattggat atcttttgct tttaaactct ctaattgagt
     8761 ttatttcaac ttttggtact actggaaaag gaatgagatt tctagattta ggatttggat
     8821 tttattttac tgttccagta tttgtatatt ttataacttc tctgatgagt tttgtaattg
     8881 cagatggatt tgtaaaggca ataaagataa aagaggataa tgatttaact atttaggtgt
     8941 gacactttaa tatataggag ataaataacc cgaaaagagt gactaactag taatttaaaa
     9001 taaaatatat tagggtataa tacataaact ataatgctaa aattaaaaga ttataatact
     9061 aaaatataat tatgttttaa ttaaaataaa gaactttatt agtagatatt aaaagcacat
     9121 ttttttataa aagtactttt ttataattta tgaaaggtat aattataaaa gtcttattca
     9181 ataaaattta taattaattt agatatattt gta
//
LOCUS       DBGJFU010000144         8997 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.146, whole genome shotgun sequence.
ACCESSION   DBGJFU010000144 DBGJFU010000000
VERSION     DBGJFU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.146"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(166..774)
                     /locus_tag="ACOIAS_003229"
     CDS             complement(166..774)
                     /locus_tag="ACOIAS_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HGZ2702919.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(883..3621)
                     /locus_tag="ACOIAS_003230"
     CDS             complement(883..3621)
                     /locus_tag="ACOIAS_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2702920.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINVCISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3769..4560)
                     /locus_tag="ACOIAS_003231"
     CDS             complement(3769..4560)
                     /locus_tag="ACOIAS_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2702921.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4596..5252)
                     /locus_tag="ACOIAS_003232"
     CDS             complement(4596..5252)
                     /locus_tag="ACOIAS_003232"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HGZ2702922.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5245..6210)
                     /locus_tag="ACOIAS_003233"
     CDS             complement(5245..6210)
                     /locus_tag="ACOIAS_003233"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702923.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKNLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6316..6422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6712..7656)
                     /locus_tag="ACOIAS_003234"
     CDS             complement(6712..7656)
                     /locus_tag="ACOIAS_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2702924.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSASIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLVKSKVLELYNHHCI"
     gene            7767..8234
                     /locus_tag="ACOIAS_003235"
     CDS             7767..8234
                     /locus_tag="ACOIAS_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HGZ2702925.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8404..>8997)
                     /locus_tag="ACOIAS_003236"
     CDS             complement(8404..>8997)
                     /locus_tag="ACOIAS_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702926.1"
                     /translation="LKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNNEG
                     YMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRDKI
                     QSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQNDF
                     IKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2933 a         1491 c          871 g         3702 t
ORIGIN      
        1 cgccaaaata tatctttctt tatatcatga aaataattat aaatttatta aatatgtttg
       61 taattctata aagttatttt tatcaaacta aaaattctat aaaaaaataa gatacatacc
      121 tataaaatac cttcctatag atacatatct tacctatata aaaacttatt ctatataaaa
      181 acgccataaa aaatcttttg cttcttctgc ataatctata tttatacgtt tactagtttt
      241 tatatctaac tcatctttac taaatacttt ttttccttta tcatcataat aaaaatcact
      301 tatatataat tcttcaccca ttatactctt actatttaga cttctatcta tttttaaagc
      361 tttgcaaagt tttcctggtc cgttagtaat attttttact tgatatttac tcaactcagt
      421 gtaagctttg ttgtatctat tcatagatat ctcatctaaa gaagttatag gctctacccc
      481 tcttattaat acacactctg gcacattttc tatatttgca gataaattca agcaattata
      541 cataccataa attaaataaa catatatatg tccaccatct aaatataatg gcttagttct
      601 atctgtcttt ttattgccat atacatgtgc tcctttatca tttacaccca tgtatgcttc
      661 tgtctctatg attttggtaa caattacttt attttcatat ttccttatta gatatttacc
      721 taatattgat ttggctaaat caattccatt ttttctaaaa aaatcctttt ccaaaatttc
      781 acctcataaa atttatttaa aacgcatcaa atacaactaa agtaataaat aaaattaact
      841 cttattaatg tgatatatat aaacaataaa tttagacatt tattatttat atatatcaaa
      901 aaattctatc tcaaactttt caatattatc attgtaattt gaattatcat atacttcttc
      961 aacaacctct ttaattggga atataacttt tacattagtt ccttctccta ccttactatc
     1021 tatgtaaatc ttcccatttt gaatttcaat caatgatttt gtaaggcata aacctatacc
     1081 acttccaaat tcatttgata taccactgtt ttcaatttgt ttaaatcgat taaatatatc
     1141 atttaatttt tctttgtcta tgcccactcc agtatctttt ataattatct ctactgtttc
     1201 gtctttttta tatataccta caaggatttc tcctccttct aaagtaaatt ttatagcatt
     1261 tgataatata tttagtatta ttctttctat cgattctggg tcaagcccta ccataacagt
     1321 ttcttcatct gtatcaaaaa ttaaatctat tcctttagac ttagcataac tactaataga
     1381 tgtaactatt ctttctgtta tgtcaataat atcaaaatta actgctttaa tcttatatat
     1441 gtctgactta acttgagaaa tatttattat attatctatt aatctcaata atctaaacat
     1501 attttgttta gttattttta tatgttcttt tagtatatcc tgaaaatcaa ttatttttcc
     1561 ttttttataa aggttttcaa taagttgaac tgaactatat atattattta aaggtgtctt
     1621 aagttcatga gatatattag cataaaactc catcttcatt tggtcatact gtctttgttt
     1681 tattaatgct tttttaagtc tttcagactc tctttgcaca cttaaatctt taataattct
     1741 cactctactt cttttctgtc caaagtataa tgttcttgat atcattttta cttctttaat
     1801 gtgattattt tcatttacta tatggatact cttctctctt tctcctatat ttaaattgtt
     1861 tatgtatttt tttactaata tatttctata tttttgtgtt gtaactccta atacatcact
     1921 agaaattccg aacaactctc taaaggctct atttgtatat agtactcttg atgtacttaa
     1981 atcctcaaga aaaatagcct ctggtataac atccagtaat ttttcatata ttattttttg
     2041 ttcatttact gttctaagaa tatttatctc tttagttata tctctaaatg taagcaattt
     2101 aaagagttta ttatctgacc aatagtctcc aactctaact tcttcaacat gctcttcacc
     2161 ttctgagtca gacatcacaa aattaactat acagtttgct gtgtcattgt aacttactat
     2221 ttcagctttc ttttcttgtg ttagatttaa tcttttaaat tccctattgt caaagaaatc
     2281 tatgttaaaa gtttcaaaat attgttctaa tgtcatatcg actaaatcac ttgaaacaca
     2341 tttaaaactc ttttcatatt ttttatttat acttattacc ttattcttct ttatgccacc
     2401 ttcagcactt cttacaagta taggagatgg aatatttagt ataatgtttt catagtcttt
     2461 atttatattt cttatttctt catctatttt gatttcattt gtaatatcat ttaaacttat
     2521 taagtaattg ctttcatctt tattattact taaacttaac aaataaattt tatcatttaa
     2581 ctctaccagt ttatcaatta tacttatatc gttatcaaat ttcttcaatt tttcagcaac
     2641 tttttcatct tcctttataa aagttaatat tttataataa ttattcatat cctttgaaaa
     2701 ttcatcttta aatttattat ttgtgtaact tatattaaaa tcatagtctg ttactatcca
     2761 tacatttggt attgtatcca gagcttgtct aagtaattct tttctctggt ctatgtagtt
     2821 ttttaatgtt ctttgtataa tcatatcctt ttctattgct aaatttatct ttcctaattg
     2881 ctctgacttt ttacttaatt ttttattgat tgaatcaagt tcataatatg gatacactac
     2941 tctattatta agactgtata catatacata ataagtatgg ttacataata gtaaatatat
     3001 tactcctatt aatctatctg aatctactat attaatctta gaaattgcta caaaacatat
     3061 aatagttgta aaaaaaacaa aatatgttaa atcttttatt aacttatagt gctcaatgtt
     3121 actatcaagt ttattcaaca aatatagtaa attaaacata gatatacaaa cattaattaa
     3181 tataataatc atattccagt taatatcact aaaaatattt cttataaagt aatctaatat
     3241 cacaaaagca ctataaaaaa ttagtattgg gcttattctt agttttttta ctttagaaat
     3301 ggttaagtat ataaaagtag acatattaat tatgcttaag ttaagcaata caatttggaa
     3361 tttttgaatt tttaaattta ctactcctaa aatacttaca aaaactaata caagacaccc
     3421 tactttcaat atatttataa attcatctgt tttaaagcta cttatataaa ctatagaata
     3481 tccaaatatc caaagtaaaa ttattctata catatttata gaaactgtat acaaatttat
     3541 atttaaaagt gcaataacca aaaataaccc attagctagt atatagacct tattcttact
     3601 taaagttcct ttagattcca tattccccac cacttcttca tttttatata gttttaccta
     3661 taaatatgtt accacaattc ttccaaaaaa tttgtcactt tctagttata aaaaaaaatg
     3721 agtaaatatt tttaatttta ctcattttgt agattttgta tattcagttt aaaaagctgg
     3781 aactatactt cctttatatt catcttgtat aaatttttta acttctttac tattcatagc
     3841 ctctgataaa gccttaatct tatcactatc tttattattt tctctacaag ctattatatt
     3901 tacatatgga gaatcacttg attctattac tattgcatcc ttagtaggat ttaagttagc
     3961 tgttaaagca taattagaat ttattactgc tccgtcaaca tcttttaata ctgttggtaa
     4021 ttgctctgca ttcatttctt ttattactat atttttagga tttttagtta tatctttttt
     4081 agttataagc tctccatctt ttacttctat caatttattt tttgctaata atttaagtgc
     4141 tctagctcca ttagttgcat catttggcac agctataaca gctccatctt ttaattcatc
     4201 taatgactta actttttctg aatagaatcc cattggctct atatgaactt tagatgtata
     4261 agtaagttta tatcctttct ccttcacagt ttcctctaaa aatggaatat gttgaaagaa
     4321 gttagcatcc aaagaccctt catctaaagc tgtatttgga agtacataat catcaaatat
     4381 ttttacttct aaatcatatc ctttttcttt taacaaaggt tttgcaactt ccaatatctt
     4441 tgcatgtgga tttgatgaag ctcctactag tattttttta tcctctttgt ttgaacaacc
     4501 tactgctgat attgctattg ctgacaccaa tgctactgat aataatttct ttaatttcat
     4561 ttttactccc cctctttaaa atgtttataa ttaatttatt ttagtttctt ataagccaga
     4621 tttcctactc cctggattat ctgaactaat attataagtg ctattacagt atataccatt
     4681 actgctgtat caaatctttg atatccataa ataagtgcta tattacctaa tcctccagct
     4741 cctactgcac ctgccatagc tgtataacca agtatagata ttattgataa tgtaattcca
     4801 gatactattg aaggcatagc ttcctttatc ataactttaa atactatttg tctttttgta
     4861 gctccaaaag attttgctgc ttctatcaat cctttatcaa cttcatttaa tgagctttct
     4921 ataattctag cgataaacgg tgctgcccca atagttattg gcactattgc agcagtttcc
     4981 cctatacttg ttccaacgat taatctagtt attggaatca ttgcaactat aagtatcata
     5041 aatgggaaac ttctaaatat atttactata aatcctaatg cactatttat tgtgctattt
     5101 ggttttaatc catctggttt tgtaactact aatatgattg ctaatataaa tcctaatatt
     5161 gttgccacta tagttggtac tataaccata tacaaagttt gtaacaatgc atttggaaat
     5221 agtgttgtta aaaagtcaat taaactattc atttatcatt tcctcccatc ttatgccctt
     5281 actagttaga tattgtttta cttgctctcc acttttatct gaaatgttta taataagtga
     5341 acctaaaata tcatctttaa attgttctaa tttcccaaat attatagaca catctatatt
     5401 tagttctctt gccatagtcg taataatagc ttcattagaa atatctttag ggaatagtat
     5461 ctttatattt gttccttttg gcaatattat actttcttca cctattaatt ttctaagtcc
     5521 tttagtattt cttaagaata tttcttctgt atctcctacc tctaatactt ctccattttc
     5581 cattattgct actcttccac aaatctgctt tataacttcc atttgatgag ttacaactat
     5641 tattgttatt cccaaatttt tatttatatc ttcaagcaat gaaagaattg actttgtagt
     5701 atttgggtct aatgctgaag ttgcttcatc acaaagaagt acttgtggat ttagtgctaa
     5761 agctctagca attgcaaccc tttgtttttg tccgccactt agatttctgg gtttatcatt
     5821 tttcttttca gatattccaa ctacttctaa taactctaaa actctttttt taatttctgc
     5881 ctttgagtat ccaaaacatt ctaatggcaa tgctacatta tcaaatacag tttttctttc
     5941 aagcaatgaa aagtgttgaa aaatcattcc taactctttt cttaaatctc tcatttgctt
     6001 ttcattcaat gactttactt ctttatcaga aacaagtaca ctgccttctt ggtattcttc
     6061 taatccgttt atacatctca gtagtgttga tttccctgca ccactatgac ctattattcc
     6121 aaaaatttct ccagattcta tttcaatact tacatcttta agcacttgaa tctttccata
     6181 atacttatta acatttttta tgctaatcat aaaatttcct cccatatatt ctgctcagtt
     6241 tttatgttta tcctctctta tgtattacaa caaaataaaa gcccgagtat aaactcaggc
     6301 ttagaaagct atttatactc atctatcaac tatattagtt gcaggattta gcaccaatgt
     6361 atataaaata tactggttgc cgggtttcaa agggccagtc cctccaccac tcttgataag
     6421 acgttattta atttaattct attatcattt gttgctatgt tatatatgat atattcatta
     6481 atagtatttg tcaataaatt tatttaaatt ttttattttt tattatcgtt aattaactat
     6541 ttttaattag ttttctagct cctaacactt tatattttaa tgtctatata actatagaac
     6601 atcattataa gaaataaatt aaataacatc aatctatcta aatcatctgt aaagtaaatt
     6661 aagaaaatta acattttatc caaactctat cctactatta aataaaataa tttaaataca
     6721 atgatgatta tatagctcta gtactttaga ctttaccaaa tgtttaagtt ctataggttc
     6781 tattattgta gcatttttcc caaaagttaa aaaatattca gccacccagt taagtgtaga
     6841 ttttggtata tacatatcta tgtatccacc tttagacaag attttcaacc ctcttgccaa
     6901 caaaaattct gtttcacaac gttttactcc ctcatctgtc aaattaattt taatatgata
     6961 atctgtacct acttccatat tcttaagata atcatgtata gaagataaaa ccttatattt
     7021 cttacttgat aagttttcta tactcaaaat cttcacaatt ctgtctacac gaaattcctt
     7081 aatagatgct gattttatac agtaagctgg acaataccaa agtccattca tggcatacaa
     7141 accaatagga acaatggtac gtacactatt tttttgcttt gatgaatatt caattttaat
     7201 tgtagattca ttcattacta tattaaacaa ttctttcaat aatggtgttg aacagtgcct
     7261 atctggtgtc caaaaaacaa atttacgcct tatattctca atattatatt gaatatcatt
     7321 tggtatacaa tttaaaaatt tttttaaaac agaaatagtt tcttgctcaa atggcaaatc
     7381 attataatat tctagtgact gatatgcaaa gaatatagat tttacctcac tttctgaaaa
     7441 tgcaatcggt ggaagtatac gctctttaag tacatgatat ccaccagccg ctccaacttt
     7501 tgaatataat ggaaatccaa tactctccag cacctgtaag tctcttaata tagtacgttt
     7561 tgatacagaa aattcttctg ctaattctcc tgctgtaaaa cttctttttg catttattgt
     7621 tatcatcagt tcaattaaac gctcagctct tgacatattt atactcctaa tttaaatata
     7681 gtgacaataa ttgtcactat tatatgataa gatattctta catttaaagc aataataata
     7741 gtaaagaaag aagggactga tactttatgg aaaataatac tattaaggct gatgatatta
     7801 tcaattattg tctatcaaac ttagatgatg ttttattaat gaatagttgg ggggaacgag
     7861 caatttacta caatcctaat ggtattttaa agcgaggagt atatgttctt accattaaag
     7921 aaaaagacag taatcatgat aaaggttcgt tggttagtcg cccaaatgta taccgtgtga
     7981 atataggatt aaaaaaagaa acttttattg aaatgtttgg atatattcca aaacgtccag
     8041 gtgtaggtca aatagttgat atggattttg attttacaaa attggacaca atcatgccac
     8101 atcctatcta ctcatggatg ggatggatat gcgtcctaag ccctactgaa aagacatttg
     8161 agaactttaa aactttaata ggagaatctt acaattttgc aaaacaaaaa tttaaaaaga
     8221 gaaaaaacaa gtaaaaacgt atgaaaataa tccctaaata aattataata aaaatattaa
     8281 ggttacaaga taaaaattaa tatcttaata acatgcaaaa aagcgaagcc aaaaaatgta
     8341 ttatttataa ggcttcgcta tatctcaaat aatttatttc aaatttcaaa actatttatt
     8401 ctattatttc ttattatttc ctgttattcc taatctatca ttcatatctt ttatcatttt
     8461 tctaaaacca ctacctatat catttccaac atcttcagtt ccatgaacta attttataaa
     8521 gtcattttga ccttttttca tatcttcatc agatacctta tctaattttt ctattctctt
     8581 atctatgtct tttactaatc tttccccttc agcgattagg tcatcattca ttttctttaa
     8641 ctctgtatct tctgttttac catctttttt aagagatgtt acaaatgatt gtattttatc
     8701 tctactatct ttatatgcag cctttacatc acttagatat ttttcatttc ctggatattc
     8761 tttatcttta aactgattat ttatactttc tggagtatca taaatagaat atctatttag
     8821 acctgctata taatcattat agcttgttga ataataattc atatatcctt cattattatt
     8881 tacagattca ttaggatttt ttcctgtatt ttcacttggt ttttcattat ttttagtaca
     8941 tgcagttata cctatagcta atactagtgc taatcctaca aataaagttc tttttaa
//
LOCUS       DBGJFU010000145         8881 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.147, whole genome shotgun sequence.
ACCESSION   DBGJFU010000145 DBGJFU010000000
VERSION     DBGJFU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.147"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            102..164
                     /locus_tag="ACOIAS_003237"
                     /pseudo
     CDS             102..164
                     /locus_tag="ACOIAS_003237"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(345..479)
                     /locus_tag="ACOIAS_003238"
     CDS             complement(345..479)
                     /locus_tag="ACOIAS_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702927.1"
                     /translation="MNISRRAMKIIELAQKIANKRGVTVQDAWNDAMKEYKEKYEYVA
                     "
     gene            complement(690..1442)
                     /locus_tag="ACOIAS_003239"
     CDS             complement(690..1442)
                     /locus_tag="ACOIAS_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGZ2702928.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VAMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1501..3156)
                     /locus_tag="ACOIAS_003240"
     CDS             complement(1501..3156)
                     /locus_tag="ACOIAS_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2702929.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSVSDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(4428..5507)
                     /locus_tag="ACOIAS_003241"
     CDS             complement(4428..5507)
                     /locus_tag="ACOIAS_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2702930.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(5591..6247)
                     /locus_tag="ACOIAS_003242"
     CDS             complement(5591..6247)
                     /locus_tag="ACOIAS_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2702931.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            6569..6775
                     /locus_tag="ACOIAS_003243"
     CDS             6569..6775
                     /locus_tag="ACOIAS_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702932.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(6928..7356)
                     /locus_tag="ACOIAS_003244"
     CDS             complement(6928..7356)
                     /locus_tag="ACOIAS_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGZ2702933.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(7832..8770)
                     /locus_tag="ACOIAS_003245"
     CDS             complement(7832..8770)
                     /locus_tag="ACOIAS_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HGZ2702934.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         2891 a         1618 c          965 g         3407 t
ORIGIN      
        1 aagtagctaa acccaattta aaaagtagtt aagtcctatt taaatagaaa agaatgaaac
       61 gagaaatata ctgtataaaa gtacataaaa acatctacta attagataca actataacag
      121 aatatattta ttattataat aacaaaagaa taaaaaaaaa ttaatttagc atagccttgt
      181 taaatataga tttgaatatg aatttaaagt tatgtaaaat taaaaataca ggaatgaaaa
      241 ttccattcct gtataaaatc taacttttga gtctcagtac aatcttattt tgagacaatc
      301 ttttttgttt tatattttct tatatttaaa atataacgca tatcttatgc aacatattca
      361 tatttttctt tatattcttt catcgcatca ttccaagcgt cttgtactgt tacacctctt
      421 ttattagcaa ttttttgtgc cagttcaatt atcttcatag ctcttcttga tatgttcata
      481 aacattcctc ctacaaatta ccatatattt catatatata aatcaaggtt atatttttat
      541 gatatataat ccttctattg taagattaca gtttatatgt gtattatata tgaactatat
      601 tagaagataa ttagaatttt tataaagcta tatgattaga ttaaatataa aagctttaca
      661 attacttata aatttaaaaa gtctataaac taaactaatg tttgatatac caattttacg
      721 accataaata atgccattac taaaaatatt ggttttatca tctttgaacc tttctttata
      781 gcaactttag cacctacata agctcccatt atcatcacta atgcaaacac tatagcaatt
      841 ttataatcta cttgaccatt tatcatgaat aataacaatg aagttatatt acttgttaag
      901 tttaatattt ttgtatttgc agaagcatgt aaaaaatcat atccatatat ttttataaat
      961 ccaaaagtca aaaaagttcc tgtacctgga ccaaaaaaac catcataaaa acccattata
     1021 agagccataa gcatccctag tcttaaattt ttcttattaa cagcttcaaa attatcttct
     1081 tgacctaaat ttttagaaat aaaggtgtaa atagccacta ttaaaatcat agccacaacc
     1141 aaaacattta atatctcttc acttattgat aatacacatc tcacacctag aacactacca
     1201 ataatagtaa atggtactaa tttttttaaa aggttgttat taatttttcc agactttgca
     1261 tatctatagg cactacttat acatcctgct gatgctgcaa atttatttgt accaatggca
     1321 agatgtactg gcactccaat tgccataaga acaggcatgc ttataagtcc tcctcctcct
     1381 gctatagaat ctacaaaagc agctaaaaac ccccctatac ataaaaaaat aactgaaaac
     1441 ataaaattcc tccatcattc tctttcaatt tttcttacaa tatatattat ataatcttat
     1501 ttaatactta ttatccagca tattctttct atattcattt ggagttatac tattaaactt
     1561 cttaaatatt tgtgaaaaat aactactgtt attaaaacct actttcttac aaatatcact
     1621 aacactcatg cttctatcta acagatattt tttagattct tctatcctaa gaagatttat
     1681 atactcagtt attgaaactt tatttctttc attaaaaata gaactcaaat aatttggact
     1741 tataaaaaaa acatctgata tactattcaa atttatattt tctttataat atttttttat
     1801 atattcgttt atattttcaa ttagatttcc tttatctttt ctccaaaatg ctctattctt
     1861 atcaatacac tctttggaaa attcaaataa tacattccat aaatcttcat cactgtttac
     1921 aagtgatatt tttttattat atttatttct taaataagat ataagtttta aatcctctaa
     1981 atctctaagc actgttctag acaatacaaa tatcatttcc tgaatagtat ctctaacatc
     2041 attatatctt aatttgtttg tatctattag attataaaca ccttctaaat tcatattttt
     2101 aatttctttt tcaatataaa gttctaattc aaagggatat tcttttgaag aattattgaa
     2161 catatctatc ggattaataa tatactcttc ttctaataat ctactatttg cttttttaag
     2221 tgcctcttca agtcttatat tttcttttct actcttcaat atctcctcat cttttatact
     2281 aagttgattt tcaaaaaaat tcctcttagc tatttcctga ataaattcag acatcattaa
     2341 taaattttct tttaatacat ataatctact tttaggattt aatggaaatt cattgtaaat
     2401 tttgtttatg tattttatat caataccaga actacttgat aattcagata aatttttatc
     2461 tatataaata gacttatata ctataaaatt accacattct atatatccaa ctatattttc
     2521 ttctaatact ataggtatct ctaaactgga aacaccatac tcacaatgat acactttttc
     2581 aaagttatca ttatttatgc ttttaacaag ttttttagta cacggtttta tatttttaca
     2641 aatatcgcaa aatttatttt ctttcttagt ctcaaatatt tctttatact ttaagtcata
     2701 tacagatatt ctaagcttca atatattaga tatttgattt gcaagatttt caattatgtt
     2761 tacattaact aactcctgta tattgttttc agaaaatact ataggtattt gtctcaaact
     2821 cgaaaattta tgaggaacat aagtaattaa tattactaaa tcaattttac cagtgtttat
     2881 aatttcatgt tctgaatatg gtgggcaata aactatagat gtttccttta tattgacctt
     2941 tttaccatca attatttgta ttccttctcc ctgaatcaca tataaaagct gttcttcact
     3001 taaatgaaaa tgcttttctt ggcagtttcc cggaaaaaat cttacaattc cagcactaag
     3061 tctattgaat tgtgtttttg atggttcgtg tatccacata acctctcccc aatcaaattt
     3121 ttgtacttca ttatttgact caataaaatc caccataaac tcacctcgta gtttttgtct
     3181 aaatattttt tcttcccttc ttatattaaa ataatatcat catcattaac tttcattact
     3241 ataaaatcat catactcttt taatactcct gtaattttag ttgcacttag tttactacaa
     3301 ttcagttttt taatatattg ataaattcta taattacttt tttattttgt aaaatcagtg
     3361 ttttcccaaa ataattatat ctataaatga aagtattttt actgcttaca tcaatatatg
     3421 ttatcataat tttccttttt attgcatata taaataactg tatataacat tgtccaatac
     3481 tatatagaaa tacatctaag aaagaatatt attaaattgt tccaattaat attatttttc
     3541 ttatagaaat aaaaaactgt tggattcttt tagaaaaatt attttttaca tacctttttt
     3601 gcttaaatag tctatcttct cagctatttt gtatatacac tcttctagtt catgatttta
     3661 ttttaatatc ttgcttatat tgctgaaata attaaattct tttatctatc atattgtctc
     3721 ttatatcaca aacatattgt ttcttatatc acaaaaatga aagcattctt atatcttcaa
     3781 tatataaacc tgcttttttt aatcttgtaa taaatatagg atactctata ttctcttcct
     3841 agaaataact aataccactt atttttctat catatagaaa gtaataaatc tttttcttaa
     3901 taaaattcaa tagtaagtgg aataaaattt tatatgaata agaacattat aaaaatactt
     3961 tatacaatag tcaataaaag atattagctt caaattgtgt tgaagttgta ttatttaaag
     4021 tggaaaagca aataaaaaag aaataatggt ataagtagaa aaatatactt aaaacaaggc
     4081 gaagcctctc ttgtatctaa atcaagtaga gaattaaatc taaaaaagaa aataaatata
     4141 aaagatgact cctgtacatt acaagaatca tcttatggtt tcataatcac tccatggttt
     4201 ttaactgtct gatataaagg tctcagttca ctcatagcat gaccaccttt tccgattgct
     4261 gaccccgtca cttatggaca aaatgtccat aagtgatagt cctctcttaa aaccctctaa
     4321 atagatatat ctcaagcacc tcattgaaac cagttgctgg aaacaaactg aagcgttctg
     4381 gaatagtaaa agaaattgcc accatatcca cgttactgtc ctagttgtta ctttgctaac
     4441 ttcggagcga tttcctctaa caatacgcag tcaaattctg cctttttgct aagaaatggc
     4501 agatggtctg catctggtat aaaaaaaatc tgtttgtctg gcgccactat ttcatcaaaa
     4561 aatttcttcg ctggaagata ggggctatta tagtcatttt ctcccatgat gtagtataca
     4621 gggacctgat agtcaactcc ataatttctt atgtcagctt cttccactaa aaaccgcaat
     4681 acatcacctt gattgtcaat cgcatttgga ttgacaatgg ccgtattttt taactctctc
     4741 aatgaataat atggggttgc cagtatactg attatattgc ccaaatccat atttatagaa
     4801 agcccatact tgctttggta tgttcttact gtcataagag ctgccaaata gcttggacca
     4861 tattttgctg gcggatatgg tgcaatagct tccagcgcct tgatatcttt ttgcttccct
     4921 gcagctttca ccatttcaag tgtcttttca tagcccacgc gctcattgtc caccatatta
     4981 acagtctgcg caatcccaat gtacgcgctg taatcctccg gataggtttg cacagccata
     5041 gttcccagca ctgttcccca gctatgacct ataaggataa ttttctcctt gttgaactct
     5101 tgcttcaaat atgctgtcac ctcatggacg tcttgcagaa cacgctccgc gctcatggtc
     5161 tgcaaaactg ctgccggatc gttcgcaaag tatgttttgc ctacgtttcg ctgatcccag
     5221 ttcaccacag taaaatcatt ttcccactca tactggtaca gatgtaaaaa tggcatcatt
     5281 gaattaccag gacccccatg aacccaaagg atgatggggt tgtctgcgtt ttgtccccgg
     5341 atatataacg cctgctgtat gccgccgatc tccaccattt ccatgcggtc gattccagga
     5401 gcttctattt tatggcttgt acgtataatc gtgggggcta tcagacgcaa accggcaaac
     5461 gccagaacaa aaacagcaac tagtatgcaa agtgtgatta atatcatttt aagtaccttc
     5521 tttcatgaat ttttttagcc atagatatct cctctaacat taaaaaaagt atgttgctat
     5581 tttagttttg ttactctacc tgtcccattg gtttcctttc ataaaaaata ttaattgatt
     5641 ctataatctc atcttccagg atatcaactc ctatttgtct cataagaaac tgtaagaatt
     5701 gaaaccgttt acgaagtgta ggaaagtccc cctgaaatac atctgtatca caccaaaagt
     5761 taaagagcag caaaattacc tctgcgcatt catctgggaa ctctgtgatg atgcttctat
     5821 cctctatgcc ttctcgtaaa acatttgcta atattggtgc tagttttttc aaattatcct
     5881 gtgtaaatgc cagtatgata tgcggacttt ctatcgcgct tgtaatcatg ttactagatt
     5941 cttttatcac ctcatccgtt aaatgccgcc taactaaact tctaagcttc tcttttgctg
     6001 taagtccatg catttcaccg agccattgat ttactgtctc tattatctgt tcaaagcgcc
     6061 tttccatgac cacattaaaa atatcttctt ttgaactgaa gtgatgaaaa attccgccct
     6121 ttgacattcc cgaagcatca acaatatttt gcatacttgt tttgtcgtat cctttttcag
     6181 caaacaactt tgcagaaatg gtaatgatat tttcaatagc ttgctttgga ttgttctttc
     6241 tagtcattat ataactcctt cttattaaaa accgactgtt ggtttttatt atataagcta
     6301 ttcctgcttc tgtcaagtaa cattgtgttt atggcaaaac aataaggttt ggtggttaga
     6361 caggtattaa ttagactaat gcgactttcc cctagatgga acccttggta aatttgcaat
     6421 ctttaccggc tttgattata tctgtaagca aggcaggtat ggccacgcct gccctgtttg
     6481 accctcggat attcattcct atccctcctc tccttagcca ttacaccaaa acgccgctca
     6541 ttgtctccaa tggcggcgtt ttaaagatat gatttctcat gctgctgaaa tatgcaaaaa
     6601 atataagatg ccctttatta tttttcctac aatagaaaag aaaagtattg aagaatgggt
     6661 taatattgga gcaaatatgc tttgttttgc acaagatact acctttatca cttatgttat
     6721 caatcaatta ttatcttttt ataatgagat tgtaccaatt caaaaacaac attaaattat
     6781 aaatctaatt ttatattaaa agagactaat attttatcta tctctattaa ggatattttc
     6841 ttatgtatca taatattagt ctctcttatt attatatact gctatcatca ttagttgtta
     6901 tttatactat ttttataata tacaaattta ttcagctaac gttttgccta attctataca
     6961 ctcatctttt gcttcttcat ttgggtcttc cattgctgta attccttctc caccaataat
     7021 tgtagcacca gcattttgca tacgttcttc ccagtctctc atccattcac aatttcccca
     7081 tccatatgaa ccaaataatc caatctgttt acctgatacc atagattcta attctgaaac
     7141 aaatggctcc atatacccct cttctagttg ctctgctccc atagatgggc aacctaatgc
     7201 aaatttatct tcttctttta aatcacttgg tttcagtaag ctcacatcta aaacttctgg
     7261 tgtcttacct tttaacttca caccttcagc cacaaaattt gccatttttt cagtatttcc
     7321 tgttccactc caataaacaa tatatatttt actcattttt acctcctgta gtatttttta
     7381 attaatcaat agttagttat agctaactaa catctaaaaa acaaaagatt tctttatctg
     7441 aaatctcttt atttttctaa atatagaata tcatagttga tattgaaatt caatacttat
     7501 tgataatatt tatcatttat attttatatt cattttataa tatcaaaaca ctgtatcgta
     7561 tttctaatct ttcatagaaa aatttctaac ttatagtact tttgcatctc ttataaatct
     7621 tttatatgta taagtacata gaaaaacaca ccttttccag atgtgctatc tataatatcg
     7681 tatatttatt tttcaattct aactagctaa tatgtttata aattttatca cttaatacct
     7741 ttatacattg atgtaaaatc ttacacctaa gtaattttta ttatttgtct cgactctata
     7801 taatatagct attttatcat caaatcaaaa attatctttt actattattc ttgtatttat
     7861 agagatttaa atataagtat ccatttcctt gttccgttct aggtattcca agcgctctcg
     7921 tacattttat taattgtgca tacagttcag cttcatctaa atctctttca aactcctctc
     7981 ttgcccattc tttaattagt gctaatgagc ctgatacgta tggtgcactc atactagtac
     8041 cactcataat agcaagttta ttatccatat aagttgatat aatatttctt cctggagcta
     8101 ccaaatctat tgtagtattt gaattactaa acttttcaac caaatagttc tcatttattg
     8161 cacctacttc tattacctca gcataactgg ctggataact atactcactt gtactagaat
     8221 caccatctcc attattacct gctgcacaca ctacagaaat attatttttt actgcttgca
     8281 tgacagcatt ttttaaattc ttatcatctt tgtttccccc aagagacata gatataatat
     8341 caaccttgtt atttacagca aagttaatag cattaattat actttgatat gttccggtac
     8401 catctttatt taatgctttt gctattaata atttacaatc tggagctact cccataactt
     8461 cattattata attagatgca gctataatac ccgccacatg agttccatga ccattaaaat
     8521 cctcatatat atttttattt ccattactgt catcactaaa atttgcacca ccaattattt
     8581 ttccttttaa aagaggatga gatatatcac aacctgtatc tattatacca actacaatat
     8641 ttttaccagt ataaccttca tcccacattc ccctagcatt catttgtttt attccatatg
     8701 ggaatttatt ttttgcacct cttagatttt catttatttc atatggaatt agtttcattc
     8761 ttttattcat catatatcct cctaaaaatt ttattgctta taaaaatcat gaattttatt
     8821 ttataatagg tttataaatc aaattaaaac ctatatctac ttatttatat ttatgatata
     8881 t
//
LOCUS       DBGJFU010000146         8781 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.148, whole genome shotgun sequence.
ACCESSION   DBGJFU010000146 DBGJFU010000000
VERSION     DBGJFU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.148"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            197..367
                     /locus_tag="ACOIAS_003246"
     CDS             197..367
                     /locus_tag="ACOIAS_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HGZ2702935.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            complement(543..1082)
                     /locus_tag="ACOIAS_003247"
     CDS             complement(543..1082)
                     /locus_tag="ACOIAS_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HGZ2702936.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(1221..1853)
                     /locus_tag="ACOIAS_003248"
     CDS             complement(1221..1853)
                     /locus_tag="ACOIAS_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HGZ2702937.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            1989..3902
                     /locus_tag="ACOIAS_003249"
     CDS             1989..3902
                     /locus_tag="ACOIAS_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGZ2702938.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            3918..5294
                     /locus_tag="ACOIAS_003250"
     CDS             3918..5294
                     /locus_tag="ACOIAS_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2702939.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(5434..6084)
                     /locus_tag="ACOIAS_003251"
     CDS             complement(5434..6084)
                     /locus_tag="ACOIAS_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HGZ2702940.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            6555..8567
                     /locus_tag="ACOIAS_003252"
     CDS             6555..8567
                     /locus_tag="ACOIAS_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGZ2702941.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         3323 a         1105 c         1209 g         3144 t
ORIGIN      
        1 ttaaatttaa ttttattttt aaaataaact tctttaaaat aaataaaaat ataagcagat
       61 taaactttat tgtaaaactt taaaacaaga atatcataat aaagtttttg ttgtagaatt
      121 aatttaattg ttatattcaa taatatttgt tataatgtta ttgaattaca atacatataa
      181 aagggaggat ttcaaaatgg catataaaat tactgacgct tgtataagct gtggtgcttg
      241 tgaggctgaa tgccctgtta gctgtatatc agctggagac gacgcatatg ttatagatgc
      301 tggttcttgt atagactgtg gttcttgtgc aggagcttgt cctgtagatg cacctcaacc
      361 agaataataa ttcgttattc ataaagagac tgattttatt ttataaatac taatcagcct
      421 ctttttaatt atgtatgatt caattaatat tatttatata ctttattatg ttgctaccta
      481 attcatctta aaatactgtt agcacatatc taatactttt aactttaatc taatttataa
      541 ttttaatact cttcataaaa tctttcatat ttatcaaaac aatcaagaca tactttcttt
      601 ccttcttcaa tccttatata ttgctcagat gcaaattcac cacacacttc acatttaagg
      661 gatttaaata ttcttgcttt ttcaggaagt cttaacttag tatctgtaat ctcaaatatt
      721 tcttcaaaat cactttctaa taaatatctt ttgtatcctt ctctatccat ctcaaatggt
      781 tttgacttta catatatcct tatagatttt ccattttctc tattaaagaa atgatatact
      841 gattttccag tcatcctaag tagtaaattt cccttaccca ttgtgcaact tagcactact
      901 tgtataccat ctattccaca agcatcattt tcagaaatac atacaatttc ctcatctcct
      961 gactgtttac atcctaaata tttttttgct gcaagtgctg ctttaaatcc tatagctaac
     1021 cctggacatt catgaccatg aaattcaatt gcctttttcc ataattcctc cacaaaaacc
     1081 actctcctta atattgttta tttccataca ttatttaaat ttttatccta atatttcaaa
     1141 aagccagagg atttaatatt aaatcttctg actttctcaa tactctttac aactgctcat
     1201 tattaatatg tttaagttgt ctactttcct tctttcatta aatacgataa ggtaaataaa
     1261 ctttctgtta agtttacatt ttcaactata acacctttcg gaacttctaa atctaaaggt
     1321 gcaaaattcc atacaccttt gattccagct tttacaactc tattaataac ttcttgtgct
     1381 ccatttttag gtatacataa tacagcaata tctatattat tgttttttat aaagtcttct
     1441 aaagtatctg aatctaatac ttcaaactct cttatcttta aacctatcat tctaggattt
     1501 gcatcaaata aagcttttat ctcgaatcca gcttttctaa atcctgcata attggctata
     1561 gcttgtccta agttacctgc tcctacaaga actgcgttgt atggtcgatc caacccaaga
     1621 attttaccta tctcagtatg aagagcttct acattataac catatccttg ttgtccaaat
     1681 ccaccaaagt tgtttaaatc ttgtcttatt tgagaagcgg taaaccctat tatatcactc
     1741 aattctttag aagatattct ttgtatatcc ctatctaata agtctccaag atatctatga
     1801 tattttggga gccttcttat aactgccatt gatatatttt tatttcccaa catcttcacc
     1861 tcctaaaagt gtcctctttt ttgtttgatt cacaataaaa tatttctttt tcaaaactaa
     1921 tacatataac cttgattata ttatatcaaa acattataat tttagatagt tcaaggaggt
     1981 agaaatttat gattgttttg tcatgtaata acttaaataa aagttttggt atagattcta
     2041 tattagaaaa tattagtttt acagtaaatg aaggcgataa aattggtata atcggtgtta
     2101 atggtactgg aaaaactact ttatttaaaa taatttcagg aatttatgga tatgatagtg
     2161 gagatattta cacttctaaa gattgtgaaa tcggatattt agaacaaaat accaattttt
     2221 attcggataa tactattttg gaagaagtac tagaagtttt taaaaatttg atagaaatgg
     2281 aatcctattt aagagaattg gaggttaaaa tatctgaaga aagtacaaaa acaaactctc
     2341 caattataga aaaaatcatg gatgaatatt cacataaact agaattattt tcagatttaa
     2401 atggatatgg atataaatct gaagccaaag gagttctaaa gggattagga tttagtgaca
     2461 atgatatgga taagccaata agcatacttt ctggtggtga aaaaactaga gttcttttag
     2521 gaaaattact tcttaagaaa ccaactctat tgttacttga tgaacctact aaccacttag
     2581 actctgaagc aatagaatgg ttagaagtat ttttaaaaca atataaaggg actgtcatgt
     2641 taatatctca tgatagatat ttcttagacc aatccgtaaa tagaattttt gaagttcaca
     2701 ataaaaagct taaagtatat aatggtaact actctaaatt tgttgagtta tctaaaatag
     2761 aaaaagaact agagcttaag aaatttgaag accaacaaaa agaaataaaa aagcaagaag
     2821 aatcaattga aagactaaaa gcttatggta gagaaaaaca cctaaagcgt gcaagaagta
     2881 aagaaaaagc attagacaaa gtagatgttt tagataaacc agaagcttat aggaaaaaag
     2941 ctcgtataca atttacgcct tctgtccaga gtggtaatga tgttcttcaa attagagatg
     3001 tatctatggg ttatggagaa agaatacttt ttaaagactt ggatttagat atatatagag
     3061 gggaaaaagt tgcacttata ggtgcaaatg gtgttggcaa atctacatta tttaaaataa
     3121 taactaatga actacaacct cttagtggca atattaaatt tggaactaat gtacatgttt
     3181 cctacttcca tcaggaacaa aaaactctta atttagataa cacaataatt gatgagattt
     3241 gggaaaataa tactcatctt actcaaacta ctttgagaaa tatgttaggg gcatttttat
     3301 ttgtagatga agaagttttt aagaagattt ctacattgag tggtggagaa agagctagag
     3361 ttgccatact taaacttata ctatcaaatg caaacttatt gttattagat gagcctacta
     3421 atcacctaga tattgattct aaggaagtcc ttgaagaggc cttaactaat tatgatggga
     3481 ctatatttac aatatcacat gatagatact ttctaaatac tgttgttgat aaaattttag
     3541 ttttagatga aaatggaatt actgaatatc tgggaaatta tgattattat atagataaaa
     3601 aaaggcaaat ccaagagatg agtattatag aagaaaaaga agaaaaaact agaactcaaa
     3661 taaaagatga aaaaagaaaa gagagagaac aaagagaaat agaaaagaaa aatagaataa
     3721 aaagacaaaa tatagaaaaa gaaattgaaa agttagagat tgaaattgaa aaattagata
     3781 tacttttatg tcaagaagaa gtatactcta atccagataa agctaaagaa gttagccaag
     3841 aaaaaattaa tttagaaaat aaccttgcct ctctatatga tgagtgggaa gaatttatgt
     3901 aaaaaggaga ggattatttg caagaactat tttctttaaa agacgaaaat aaaagatttt
     3961 ataaaatact tctatcttta tgtataccta ttatcataca gaacttaatt tctacttctg
     4021 taaatgtaat agatacaatt atgataagta gtttggggga aacatctgta gcttcagttg
     4081 gagttgcaaa ccaatttttc tttcttttta atatgtcctt atctggtatc acaggaggtg
     4141 ctggagtttt tatttcacag ttttatggaa agaaggatgt gagtaatata agaaaagtca
     4201 caggtcttac ttgtgtattg gcaattgttt taagttttgt atttgttatt cctgctttac
     4261 taacaccaaa gccaattata cacatatttt cgtatgattc tgaggtggta aaactttgta
     4321 tagattactt tagtattgca gtatttagtt atccattaat tgctgtaagt actgtattta
     4381 gtactggttc aagaggtgtt agaaatccta agttaggtat gatttgtagt gcatttgcac
     4441 ttgtaacaaa tgttatttta aattatggat ttatatttgg taacttcggt cttcctgcat
     4501 tgggtgtaaa aggtgctgct ctagctactg ttattgctag aatatgtgaa ttaattttaa
     4561 tgattactta tgtttacctt tataaaaaag actatatatt aaagtttggt ctaaaaaatc
     4621 taaaagctat tgataaaatt tttataaaat ctttttcatc aaaaagcttt cctatatttg
     4681 taaatgactc tgtatgggca attggaactg tcctgtattc tgttgcatat gcaagagctg
     4741 gtacttctgc tatagcagct agccagatag caaccagtac aggtaatttc ttcataatga
     4801 cagctgtatg tattgcatct ggtgcatcta ttatgcttgg aaatgaactt ggagctgacc
     4861 atattaaaag agccattgaa tatgctaaaa agttttctat acttgtattt tcagctggtt
     4921 taatacttgg aattatttta attttaaata taccattatt attaaaaatg tttagtgttt
     4981 ctgatagctt agcttctgat ataactaaga tattctttat aatgggaata ctaatggctc
     5041 ttaaatcttt taatacattg gttataattg gtattttaag aagtggagga gatactaaat
     5101 atgctctatt tttagaatta ggatgtatgt ggttagcttc tattcctcta acttttatag
     5161 ctgcatttaa aggtgcacct atatttgtac tcgttttgct cacttatagt gaagaagtgg
     5221 ttaagtttat atttggtgtt cctagagcat tatctaaaaa atgggctatt aatatagtaa
     5281 aggaaattga ttaataatta tactatacat tataagtata caatatttct aaacagtctc
     5341 attgaaaata actaaagtat ttttgatttt cagtgagact atttctattt tttacagaaa
     5401 ttattatact ttattttgat taagtaacaa tttttactca tcttccaaaa attcttttac
     5461 ttcaaatgcc aatttatgta attcatcata gtttaaaatt tcatatgtat tacccatttt
     5521 ttttactatc tccttttcag ttaactttag cagagaatgg tatctacttt ttctactaat
     5581 tgaatagatt tcactgaaat tacgaatagc ttttatattg cagacattat tttcttgttt
     5641 atttgatata caataggcaa taacttctgt gcaagaaaat agttctctta ccattctggt
     5701 tttatataat tcatatattc ttcttgtaca ttttctgtga aatagtgcaa taaaatctgg
     5761 tttgagataa aactcttcaa atacattttt ttcaaatcga aataatttta tattagtctt
     5821 tgcaaatata tcacatttca aattatgttc atatatgtaa ctaattttgc ctataaattc
     5881 attttcaggt acaatgtcaa caagaaattt atttcctcct ctagtagtac actccactaa
     5941 tgcaactccc tctattataa agaacacttc ttgtaagtct ttatctgatg ctacaaatct
     6001 ttgacccttt ttatattcaa caattgatat tttgtttaaa ttatttttaa taattggata
     6061 tatacttgtt ttttctgctt tcatcaaagt tacctccttt acaatatatt gtataaaata
     6121 ttttatttat atgttttaat ttatgttatt tttaataaaa gatagaatta caactaaatt
     6181 aaacttgaca aatccattct actcacgcag aataaactta tacatatata ttttcatata
     6241 taatttattt taatataatt ctatatttat aaaattatat tatcaataga atttattaaa
     6301 ataaattttt ttgggtgtat attataattt aatcgaatag aaatgaaaaa tatacataca
     6361 gtttaagttt taagcaacaa ttagggggat ttggtgacaa gatttttaac tataagaaag
     6421 aaagtgtctt aatgcaactt ttagaaagtc attatagaca ctttcttttt tatttgataa
     6481 atttaaatta tcaaaattag attgctttat acaactattt tgttggaaac tatttattat
     6541 ataaggagaa ataaatgaaa aaatcagtag ctctggtaaa tgatagtaga aaagatttaa
     6601 tagacttttt agaaaataat ttgaaattag tttttggaga ttctataaat attaatagat
     6661 acttcataaa tgaaataaat gataatgata ttataaatga tgatgtgata ttagtaatgt
     6721 cagtagaaag attagacaaa attataaata atatactaga taagaaaaaa gtgattgtgg
     6781 taagaagaac ttttagggaa gataaaattt ataatctact atctttacca cagggtacaa
     6841 atgttctaat cgtaaatgat tctgatgaaa ctactctaga aactataagt ttattttata
     6901 aaattggagt gactaatatt agaccaattc catatatgaa tgataataac tataaaaata
     6961 taaaaatagc tataactcca ggagttccag aaaaagtacc tagctttatc tcagatatat
     7021 ttgatttagg tcacagatat atagatatct ctacatttat agaaataata aatttattac
     7081 aaattgattc aaaggaaata caatctaatc tagttaaata ctcagaagaa ataataagtt
     7141 tagatactgg tataaaagat aaatataagg aattattttt aaaaatagaa gaattagata
     7201 caatcttgaa tttatcaaaa gatggtatat tatttacttc aaaagatggt gaaataaata
     7261 catataatag taaagtaaaa gatattttag acataaatga ggatatatat ggaaaataca
     7321 ttgaggatat atttgtagat agtttaaaag ttttattgag tgagaaagag atactagata
     7381 aagttgtcgt attcaataag aaatatataa atgtaaataa aaaaaacata tacaatagag
     7441 atgaaaaaat gggcacatat tatagtttac aagaaataac atacataaaa aaattagaac
     7501 aaaatctcac taaaaaatta agagaaaaag gacaaatagc aaaatataca tttaaggata
     7561 taaaaacaaa tagtcctaaa atgtttgaat gtatagattt agctaagaag gtttctaaat
     7621 ctgatttaag catacttatc agaggtgaaa gtggaactgg taaagaactc atagctcagt
     7681 ctatacataa caactcaaat agaaaaaatc aaccctttat agcagtaaat tgtgcagccg
     7741 ttcctgaaaa tttattagaa agccagctat tcggatatga caagggaact tttactggag
     7801 gtctaaaaga tggaaaacaa ggattatttg aacttgctaa taatggaaca atattcttag
     7861 atgaaatagg cgatatgcct ttagaattac aaacaaaact acttagagtt cttcaagaaa
     7921 agcagataat gcctgttgga tctcacaatg taattaatat agatgttaga atcatatctg
     7981 ctacaaataa gaatttagaa caaatgattg ataatagcca atttagagag gatttatatt
     8041 atcgattaaa tacaattcca ataaatatac ctcctctaag agaaagaaaa gaagacatat
     8101 taattattat ggaagattta attaataaga aattagtaat cacaccagaa gcaaaaaaac
     8161 ttatacaaaa ctatatgtgg aaaggtaata taagagaact ccaaaatgtt acatcctatt
     8221 taaacattat gtgtgaagat attgtattag agaaagattt acctccaaac ttaaggtctt
     8281 cagataacaa aaatacatca ttaaaactaa aatatagtaa gaatgatata ttgaatatat
     8341 tagagatact aattttaaat aaggaatcag atgttggtat tggtagagga ttaattttaa
     8401 aagcactact cgataagaac cttcaaatta cagaaggaaa aattaaaaaa atatttgaat
     8461 atctaaaaaa agaagaactt ataatttgta gttcaggaag atatggaagt aaaataactc
     8521 aaaaaggaga agatttttac aataaattaa aatataaagg tctataaaat tataatttat
     8581 caaacaaaga aatctattca ggattaaagt actccttcaa taggtttttt tatttttcaa
     8641 gtaccctaaa attataattt attttgtaca taaataatag gaattaatag gaataggata
     8701 atttttttat ttcctatttt ttttatataa actagcatat aaaatatatt ttcttcaaaa
     8761 taatattttt tactgaaaat t
//
LOCUS       DBGJFU010000147         8744 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.149, whole genome shotgun sequence.
ACCESSION   DBGJFU010000147 DBGJFU010000000
VERSION     DBGJFU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.149"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(116..589)
                     /locus_tag="ACOIAS_003253"
     CDS             complement(116..589)
                     /locus_tag="ACOIAS_003253"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2702942.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            888..1655
                     /locus_tag="ACOIAS_003254"
     CDS             888..1655
                     /locus_tag="ACOIAS_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HGZ2702943.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1873..2086
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2340..3374
                     /gene="argC"
                     /locus_tag="ACOIAS_003255"
     CDS             2340..3374
                     /gene="argC"
                     /locus_tag="ACOIAS_003255"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGZ2702944.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            3415..4632
                     /gene="argJ"
                     /locus_tag="ACOIAS_003256"
     CDS             3415..4632
                     /gene="argJ"
                     /locus_tag="ACOIAS_003256"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HGZ2702945.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            4647..5507
                     /gene="argB"
                     /locus_tag="ACOIAS_003257"
     CDS             4647..5507
                     /gene="argB"
                     /locus_tag="ACOIAS_003257"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGZ2702946.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            5537..6724
                     /locus_tag="ACOIAS_003258"
     CDS             5537..6724
                     /locus_tag="ACOIAS_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HGZ2702947.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYVERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6833..7855
                     /gene="argF"
                     /locus_tag="ACOIAS_003259"
     CDS             6833..7855
                     /gene="argF"
                     /locus_tag="ACOIAS_003259"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGZ2702948.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7982..8599
                     /locus_tag="ACOIAS_003260"
     CDS             7982..8599
                     /locus_tag="ACOIAS_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HGZ2702949.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         3542 a          839 c         1563 g         2800 t
ORIGIN      
        1 taaggatttt aaagtatatt attaaaaaac aaaaaagata gtattatttt aaatcttggt
       61 ttaaaagcaa agtaactata aaatatttaa gagactttgc tttttattta atttattata
      121 ttaacaattt cataggtgta aaacgtatac cgtcaacaat ttgctcaaca gcagtatctt
      181 taaatcctaa tttatgataa acttcaacag cataaggtga tgaatttaca gttatttcag
      241 ttaaattaaa tttattacaa atacagtatt gtatgcctgt agtcattaat ttttttgcaa
      301 caccatgttt atgatagttt ttatctacaa ataacataca tatatgattt aaatttctaa
      361 tagctaagac tccaataagt tttttagagt caaaacaacc ccaaaagaag agagtttttt
      421 ctttgatttt ttcaataata gagttatatt ctataaattg atgaaatgtc gtaattcctt
      481 catcacaata atctggagat tcaaattctt caaatacgtt ccagattaag tctaatgcat
      541 ttttaatatc atcttctttt aattctctta ttaagtattc tttgaacatt ataaacaaca
      601 cctttattat tattttaatt ataattatat atcttaaata aattcaattc aatacaattt
      661 gttttataaa tttagaatat tatgataata tataatttat attatagtgt agttagacca
      721 tagaatgtat ttaattatat atacaattta ataattcaga taatcttaaa ataatattat
      781 gtaaaataaa aatatatatg acatgatgtt gttgtattca aaatattaag ataaaaatat
      841 atagaatata tttaaaaatt attatgcaat tagaaaagga gataagtatg cagattattg
      901 atataaatat aaataatatt gacaatgaac atatatgttg ttcaatttct gaaaaaaaag
      961 gagaaaattg tgttttttct aaaaaagctt ggatgaaaaa taattttgat aaaggtctag
     1021 tctttaaacg atttgatgta agaggtaaag cttttataga atatattcca gcagaaaatg
     1081 catggtgtcc tattactgcg gacaattata tatttattaa ctgtttttgg atatctggaa
     1141 aatttaaggg tcaaggatat tctaatctat tgctacaaga gtgtgttgat gatgcaaaat
     1201 caaaaggtaa atatggatta gttgtgcttt cgtctagtaa aaaaatgcct tttttatctg
     1261 atgctaaata tctaaaatat aaggactttt gtttagcaga tacatcagaa ccaaattatg
     1321 agctattata ttttcccttt actgataatg ctcctattcc atgttttaag aattgtgtaa
     1381 aacaaagaaa attaaatgag aatggtatag ttttatatta cagtaatcag tgtccacata
     1441 cggaaaaata tgctcctatt ttgaaaaaaa ttgcaaattc tagaggtatt gatgtaaatc
     1501 ttataaaatt cgaaacagca aaagaagcac aaaatgctcc tgcaccattt actacataca
     1561 gtcttttttt taatggtgaa tttgtaacca acgagatact ttctgagaaa aagattgaga
     1621 aatttttaga taccaacatt aaaaaattta attaaaaaga gatgtaaata ttgtattttg
     1681 aaaatatatt attttataat ttaatatatt ttggatgttt tttaaattga cgaatgaaat
     1741 ataaaaatta tatcgcaatt tagatacaaa atgctggaaa ttaaccatat agaaaatatt
     1801 tccaaaaatt taaaaattat attgacaaag ttaaaaaaat aaactatcat ataaaacaat
     1861 aagaaaataa attgaaatcc tgtgaaaaag agagtagcat aaagctagtt gatagagagc
     1921 tgggaatggt ggaagcctag taacgaattt tattgtgaat agagctttgg aggagattac
     1981 cagaagtaat agtatggtaa ttggtgaatc tcgccttaag agattagagt gggtaagatt
     2041 atcttatcaa ttagagtggt aacgcgggtg tatattcgtc tcttggtctt tgaccaagag
     2101 gcttttttaa ttttaaatgt acattttgta gatacttttt aatttgagtt ttaatgatta
     2161 ttgtaatttt accaataaga gtggtaccgc ggaaaatcgc ctcttagtat ttataatgct
     2221 aaggggtttt ttgttttaat tagagtattt taatctacgt attatcaaat gattatcata
     2281 tagtaaagtg gaaagatagg attttaagac tatttgaata taaaaatgga gggaacaaaa
     2341 tgaataaaat aaaagttggt attattggtg caactggata tgtcggggca gagttgatta
     2401 gattattaat gaatcatgat aaagtcgaag tgacagcgat aggttcaaat tcgtatgttg
     2461 gaaaagatat agttgatata tatccaagta taggatataa aaataacatg atatgtatag
     2521 aaaatgaaaa agttatagat atgtgtgatg ttgtgtttac agcccttcca catggagtta
     2581 gtgaaaagtt tgtaataaag gcaattaaat cgaaaaagaa agttatagat ttaggtgctg
     2641 actttagaat aaaagatgaa gaagtatata gtaaatggta tggagtatct tttatagata
     2701 aaatcttaca taaaaaagct gtatatggat taagtgaaat atacaaagaa gatataaaag
     2761 atgcagatat aattgcaaat cctggatgtt atccaactag tatttcttta ccattgatgc
     2821 cattactaag ttctaaattg ataaaaaata ataatataat aattgattct aaatcaggtc
     2881 ttacaggtgc tggtagagaa ttatcgatta gtagtcactt tacggaggtt aatgaaaata
     2941 tcactgcata taaaataggg aaacacagac atactccaga aatagaacaa aatttaagtg
     3001 aatcctgcaa agaaaaagta agtgtagttt ttacaccaaa cttaatccca gtaaataggg
     3061 gaatcttatc aactatatat tgtacaaaag aagataatat ttctataaat gatatacata
     3121 ggaaattaac tgattattat gaatttaaag aatttataga aattcttcca ttagataaga
     3181 tagcttcttt aaagaatgtt agattctcaa ataaatgtgt aatatcatta catgaaaatg
     3241 gagatacatt gataatctgt tcagctatag ataatatgat taagggagca gcaggtcaag
     3301 ctatacaaaa catgaatata atatttggta ttgaagaaaa tacaggttta aaaaatattg
     3361 caccatcatt ttagaaataa aaaatactaa actcatataa ttaggaggat aaatatggag
     3421 attataaagg gtggagttac agtttcagaa ggattttttg cttcagggat tcactgtggc
     3481 ttgagaaaaa ataaagaaaa aagagattta gctttggttt attcagatgt tttatgtgat
     3541 gcagcagcag tagtaactca aaataaagta aagggcaatc ctgtttatgt tacacaggaa
     3601 cacattaaaa atggaaaagc acaagctatc atagtaaata gtgctaatgc taataccttt
     3661 aatggaaaag aaggacttat aaatgcttat aaaatggcta aattcaccag tgataaatta
     3721 aatattaaag aaagtgatgt tttggttgca tctacaggtg ttataggaaa gccattaaat
     3781 atagatttaa tagaagaaaa tatggatgaa ttagtgaata atctaagtaa acaaggtcat
     3841 attggagcaa gagaagctat tatgacaact gatattataa aaaaagaaat agcagtagca
     3901 ataatgtatg gagataaaaa aataactatt ggaggtatgg caaaaggttc tggtatgata
     3961 catcctaata tggcaacaac gttaggattt ataactacag atgcaaatat tgatggagtt
     4021 ttgctaaaag aagctttaaa aattgctgtt gacaaaagtt ttaatagagt tagtgtagat
     4081 ggagatacaa gtactaatga tatggtaatg attctagcaa atggaaaagc aaaaaatgat
     4141 agaataaaca aaaaagatga acattatcaa gtatttttga gtgcacttac atatgtctgt
     4201 atagaacttg ctaaacttgt tgcaaaagat ggagaaggat gtacaaaact aattgaatgc
     4261 tatataaatg gagcattaag tgaagcacat gcagtaaaat tagcaaaaac agttatatct
     4321 tcttcattag taaagacagc tgtatttgga gctgacgcaa attggggtag aatactgtgt
     4381 gctttaggtt atgcaggtga agaaatagat atggaaaaag ttgatattat ttttgagagt
     4441 atgaaaggat atattgaagt atgtaaaaat ggaaatggat tagattttaa tgaggaaaaa
     4501 gcaaaaaaaa tccttgaaga tgacgaaata agtatattag tagatttaaa tatgggcaat
     4561 gccagaggaa atgcatgggg atgtgattta agctatgatt atgtaagaat aaatggaagt
     4621 tatagaaatt aggaggatat actttaatga ttaatataga aaaagcaaat acacttatag
     4681 aagctttacc atatatagaa aaacatcagg gaaagactat tgtagttaag tatggtggaa
     4741 gtgcaatgaa aaaagatggt ctaaaagaat ctgttatgga agaccttgtg ttaatgagtt
     4801 atgttggaat caatatagtt ttagtacatg gtggaggggc agaaatcaat aagatgctag
     4861 caaaagtgga tatagaaagt aaatttgtaa atggtcttag atatacagat gaagagacca
     4921 tggaaattgt aaaaatggta cttgctggaa aggttaataa agacctagta aataaaatac
     4981 atacaaaagg tggaaaagca gtagggcttt gtggaataga taataatatg attttatgtg
     5041 acccatataa aaattatgaa cttggatttg taggagaaat caaaaaagta aatgttgagc
     5101 ttatagaatc ttgtctaaag tcaggttata tttcagtaat tgcaactata ggtgttggag
     5161 atgatggaga gacttataat ataaatggtg acacagcagc ttctgctata gctaaagagt
     5221 taaatgcaga taaattgata cttcttacag atgtccctgg tttgttaaga gagccagatg
     5281 aagaaaaatc attaataaca gaagttatat tagaggatgt tgataaatta tttgaagaag
     5341 gtataattac tggaggtatg atacctaaaa tagagggatg cgtagatgcc ttaaataacg
     5401 gagtaaatag ggtacatatt ttggatggaa gagttccaca ttctataata acagagctat
     5461 ttactgatag tggaataggt acattaataa gaaaagaaaa tgaataaaat tataagttta
     5521 agaggggaga ataagaatga ataacgacaa tacaatttca aaatggaatg aatattttat
     5581 agatacatat aatcaaccca attttgtcat agattatggt gaaggaagtt gtttttttga
     5641 tactaatggc aataaatata tagattttac aagtggatat ggagtatctt ctcttgggta
     5701 tagtaatagt aatttaaaga atgctctaaa agagcaagta gataaattat tacatacttc
     5761 aaatttatat tttaatgagc cagtgttgta ttcaggagag aaaataatta attcaagtgg
     5821 aatggctaaa gtatattttt gtaattctgg aacagaagca aatgaaacag cattcaaaat
     5881 agcaagaaag tatagtagtg acaaatatgg taatggtaga ggaactatca taagtttaaa
     5941 agactctttt catggtagaa caatgatgtc tttaatggca actggtatgg acaaatacca
     6001 taagtatttt tatcctcttc ccgaaggttt taaatatgta gaaagaaata atattgaaga
     6061 tttaaaaaat aatcttgatt ccacagtttg tgcaataatt cttgaagcga ttcaaggcga
     6121 aggtggagtt aatgttcttg aaaaagatta tgttttggaa atagtaaaaa tatgtcaaga
     6181 aaaggatatt gtagtgattt ttgatgaagt ccaatgcggt ataggaagaa ctggtaaact
     6241 ttttggctat gaatattttg atgtaaaacc agatatagtt accgtagcaa aagggttggg
     6301 agcaggaata ccagtaggag gagtattagt aaataaaaaa ctttcaaaag tactaggaaa
     6361 aggagaccaa ggtacaactt ttggaggtaa tttattagct atggtggcag cttcagttgt
     6421 attagatgag atatctaaag atggttttta taatgaagta ttagaaaaag gaaattatat
     6481 aagaaaatct attgaaagtt ttaataacaa agttgtatta aagacaaaag gtattggtct
     6541 aatgatagga attgaaacta atattgaatc tagtattata gaagagaaag ctagaaaaaa
     6601 aggattgctt attttgactg ctggaaaaaa tgtattgaga tttttaccac cattaacaat
     6661 cagttataag gaaatagatg aggctcttga aatcctaaaa gatatattac tagaaattaa
     6721 ctaaatattc aagaaatagt tgattaaaga taaaaattag aagtaaataa aaaagtaaag
     6781 tgctaacaag tttaattgaa tttaaaaagt ttataagaag gggcgaatta atatgaataa
     6841 tataagttta aaaggtaaga gttttttgac attaaaggat tttacaaaag aagagattag
     6901 atatttatta actttatcaa gttatttaaa aactaagaaa aaagttggta taaaaggcga
     6961 tttgctacaa ggaaaaaata tagctttatt atttgagaaa acatcaacaa gaactagatg
     7021 ttcatttgaa gtagcagctc atgatgaagg agcacatgta acttatctag gtccaaatga
     7081 ttgtcatatg ggtaaaaaag agtctgtatc agatacagca aaagttctag gaagattcta
     7141 tgatggaata gaatttagag gctttaaaca agaaacagtt gaaagtctag caaaatattc
     7201 tggtgtgcct gtttggaatg gacttacaga tgaatatcat ccaacacaaa tactggcaga
     7261 ttttttaaca gtaattgaaa atgtagataa agatttgaat aaagttaaat ttgtatatgt
     7321 tggtgatgct agaaataata tgggaaactc actgatgata ggatgtgcta aaatgggaat
     7381 acatttcgta gcactagcac ctaaggaact ttggccaaat gaagaattgg ttaaggaaat
     7441 gaaggaaata gccaagaaat ccaaaggaga tataatgttg actgaaaata ttgatgatgt
     7501 taaaggtgca gatgttatat atacagatgt atgggtttct atgggagaag aggagcaata
     7561 tgaagcaaga ataaatcaac ttaaagatta tcaagtaaat atggatatga taaagaaaac
     7621 tgaaaatgaa aatgttatat ttttacactg tcttccagca tttcatgatt tagaaactaa
     7681 ggttgctcag gagatttatg aaaagttcgg attatcagaa ttagaggtta caaacgaggt
     7741 atttaattca aaatattcaa aggtatttga agaagctgaa aatagaatgc atacaataaa
     7801 agctgttatg gtttctacta tgactgatgt aaatatgaac aataatttaa tatagatatg
     7861 ttttaatata gactttactt taatgaaaat ttataaaata ttttaaatat attattcaaa
     7921 aactctcatt atatgttaaa ataacttatg tatcaacaaa ttaagaggga ggatttgaga
     7981 tatgaaaaaa atcatattag ctttaacaac ttttttatta gcaatatctt taacagcatg
     8041 ttcaagtgca aaaccagaag atacaattga tagttttttt aacagtgcga aaaagtttga
     8101 ctttgaaggc atgaataagg taatggaaaa taatgacgaa aaatataagg atatattaaa
     8161 ggaattagat acaaaagacc caaatgccca atatgtttta gattatctaa aacagaatgc
     8221 atctaaaatc acatatacaa taaaagacag cgaggttaaa ggtgataagg ctacaatcaa
     8281 agtagaatgt aaatttatag attcaacacc attacttcaa gaaatagtag ctgaggcatt
     8341 tacaaagatg attggtatga gtttttcagg acaagactta actgatgaga aaacaactga
     8401 aatgttagta tcaattatga aagaaaaaca aaaaagcgtt aaagagactt atgttacaaa
     8461 aaatgtagag tttgaatgtt ctaaaaagga taataaatgg ataataagtt cagtaaatga
     8521 tgcagtagct gatgtattgt tatctaatct tgttacagca ggacaagaat tttctaactc
     8581 tatgaataca cttgaataat attgtttagg tgtagtaaag taataaatat atgattattt
     8641 ttataaataa acatataaat atttatgaag gataccattt ttggtatcct ttttattaat
     8701 ataatgaaat acatggttaa tttctatatt gtcaagaaac tatg
//
LOCUS       DBGJFU010000148         8702 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.151, whole genome shotgun sequence.
ACCESSION   DBGJFU010000148 DBGJFU010000000
VERSION     DBGJFU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.151"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(28..543)
                     /locus_tag="ACOIAS_003261"
     CDS             complement(28..543)
                     /locus_tag="ACOIAS_003261"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HGZ2702950.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(906..1976)
                     /gene="leuB"
                     /locus_tag="ACOIAS_003262"
     CDS             complement(906..1976)
                     /gene="leuB"
                     /locus_tag="ACOIAS_003262"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGZ2702951.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(1983..2474)
                     /gene="leuD"
                     /locus_tag="ACOIAS_003263"
     CDS             complement(1983..2474)
                     /gene="leuD"
                     /locus_tag="ACOIAS_003263"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGZ2702952.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2489..3766)
                     /gene="leuC"
                     /locus_tag="ACOIAS_003264"
     CDS             complement(2489..3766)
                     /gene="leuC"
                     /locus_tag="ACOIAS_003264"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGZ2702953.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3723..5465)
                     /gene="leuA"
                     /locus_tag="ACOIAS_003265"
     CDS             complement(3723..5465)
                     /gene="leuA"
                     /locus_tag="ACOIAS_003265"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGZ2702954.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5589..5835)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5983..6429)
                     /locus_tag="ACOIAS_003266"
     CDS             complement(5983..6429)
                     /locus_tag="ACOIAS_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702955.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6450..7169)
                     /locus_tag="ACOIAS_003267"
     CDS             complement(6450..7169)
                     /locus_tag="ACOIAS_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HGZ2702956.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7287..8561)
                     /locus_tag="ACOIAS_003268"
     CDS             complement(7287..8561)
                     /locus_tag="ACOIAS_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HGZ2702957.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         2647 a         1721 c          881 g         3453 t
ORIGIN      
        1 gttttaaaat gtaaaatttt tatttaatca aagttcaata tattccactt tttctttcga
       61 ttcttcaaaa aaccattctt cattagaaaa atcaccttct gcaatttgtc caataggcat
      121 tttcatatac tttccagttg ctgtcactgc tatatctcca tttggtagaa ttatttctcc
      181 tgtaccttca aataattttc tgttttcttt agttattctt ccaacaactt ttatagtagc
      241 atctgttggt actggtttct tatatttcaa ctctaaagat actgttactc cccatatttg
      301 gtcatcattt atacttacag ctcttccaat agtctcatcc aatattgcag cagacatacc
      361 tccatgaact ctcccaggat aactttgatg ccaatccttt gtattacaaa ttgatacaag
      421 ctctccattt tcaagttcat aaaaatctgc tttaagtcct agctcatttt gtgttccaca
      481 aactaaacaa gatttactac tattttgctt cttcaaaact ttatactttg ccaaaaaatc
      541 catctcctaa ccgacattag tagtcgctta atattaaata attctaaaat atattatata
      601 atattaataa aatccctgca aacttctaat aataaaaata ggctacttca atatgataag
      661 tttaatttaa agtattattt tccaaaaaat aactttatca aaatagaaac tcaaagtaga
      721 tataaacctc tttttcgtta ttaaaatagt aaaaatatat ttgacttaat ataaaaaaga
      781 gtgtctcatt aactaataaa gtttattttg agacacccct cataaaacaa attatactac
      841 aatagatttt tactaattct acaagttata tatttaaatt atattattca aattaaacta
      901 taattctaaa tttcatttac tattaattcg cccatttccc ttgttcctac aacatttccc
      961 acaccattat agatatcaat agttctataa cctttattga gtacactttg tacagcatct
     1021 tcaatacact tagcttcttc ttctaagtta aaactatgtc ttaacatcat gcttacagat
     1081 agaatagtcg caattggatt tacaatatct tttccagcta tatcaggtgc acttccatgt
     1141 attggttcat acataccaaa actatcttct ctaagtgatg ctgatggtaa catacctata
     1201 gaacccgtaa tcatagatgc ttcatcagat attatatctc caaatatatt attagtcact
     1261 attacatcaa attggctagg gtcttttaca acttgcatag cagcattatc aacatacata
     1321 tgtgacagtt ctacatcttc aaactcttta gctaagtctt ccattatact tctccaaagt
     1381 cttgaactct caagcacatt tgccttatct acacttataa gttttttatt tctatttctg
     1441 gctatttcaa aagctctctt accaattctt cttatttcat tttcgttata tatttctaca
     1501 tcataagcat tttcgatacc atcaatgatt tttcttccac gttctccaaa ataaatgccc
     1561 cctgttaatt cccttacaac aacaaaatca atgcctttct caactatatc cgttctaagt
     1621 ggtgaagctt cttttatact ttcatacatt gtagctggtc ttaaatttgc aaataaaccc
     1681 aatgactctc ttaattttaa aagaccagac tctggtcttt tatctccctt acatttatcc
     1741 cactttggtc ctccaacaga accaagcaat acagcttcgt tttttcgaca agtttcaaca
     1801 gttttattag gtagaggttc tccagtttca tcaatcgcgc atcctccagc taatacatat
     1861 tcacaatcga atctatgatt aaatttacaa caaattttat ttagaacctt tataccttca
     1921 tctataattt ccggtccaac accatcacct tttatgactg ctatattaca attcatactc
     1981 cactaccccc tatttgcttt tattgagtta attaagcctt catttaatat aatattttgc
     2041 ataaactcag gaaatggttc tgctttatat tcttttcctt ttgttatatt ttttatcaca
     2101 cctgtagaaa aatctacttc tatattatcc ccttcatcta tgttattggc agcttcttca
     2161 cattcaagta taggaagccc tatatttata gagtttctaa aaaatattct agcaaaagta
     2221 gaagcaatca cacaggatac tccactttct tttattacaa ttggtgcatg ttctcttgat
     2281 gaaccacaac caaagttttt agttgcaact ataatatctc ctttttttac cttagaaata
     2341 aatttatcat caatatcctc catacaatgc gttgctagtt ctttataatc agctatattt
     2401 aaatatctag ctggtattat tacatctgta tctatattgt caccgaattt aaaaacactt
     2461 ccattggcaa tcataaattt tacctccctt aaattatttc tgatggtttt gatatttttc
     2521 cagttatagc tgatgctgct gctacagctg ggcttgcaag atacacctct gaatcaacat
     2581 gccccattct tcctacgaaa ttcctatttg ttgttgaaat ggatttttct cctgctgcaa
     2641 gtatacccat atgtccccct agacatggac cacaagttgg tgtagaaact gctgctcctg
     2701 cctctataaa agtggtaata tacccttttt ctatagcttc aagatatact ttttgagttc
     2761 caggaaatat cataactcta actccatctg ctactttttt gcctttcatt atctcagcta
     2821 ctacttcaag gtctgatatt cttccatttg tacaagagcc tattacaact tggtctatct
     2881 ttatatcctt atctatctca tctactgttc tagtattttc aggaagatga ggaaaggcta
     2941 ctgtttcttt tagttcagct aaatctattt cgtatacttc atcatagact gcatcttcat
     3001 ctgcttcata tatatttaca tcttttgtca tagttgaaca cttatgtgaa tttatatatt
     3061 ccatagtctt atcgtctact ggaaaaattc cattttttgc tcctgcttct attgccatat
     3121 tacaaatagt aaatctatca tccatagata agtattctac tccatctcca caaaattcca
     3181 ttgacttgta aagtgctcca tcaacaccta tctcccctat tatatgaagt attatatctt
     3241 taccacttat ccatttattt ggtttattct tcaaaacaaa ttttattgct ccaggaacct
     3301 taaaccaaca ttttcctgta gccatacctg ctcccatatc agttgaacct acaccagtag
     3361 aaaatgctcc taatgctcca tatgtgcaag tatgagagtc agcacctata attacatctc
     3421 cagcagttac tatacctttt tcaggaagaa gaacatgctc tattcccata tctcctacat
     3481 caaaaaaatt tacaactcct tttgattttg cgaaacttct tgtaaattta cattgttctg
     3541 cactctttat atctttatta ggtgtaaagt ggtctaaaac catcactact ttagtcttat
     3601 cataaacatc ttcaattcct aatttttcaa attctctaat ggctactggt gatgttatat
     3661 cattacctaa aactacatct aaatctgctt cgattaattg ccctttctta acttcattta
     3721 atttagcatg tttggctaat attttctgcg tcatagtcat ccccattttt tatttcctcc
     3781 tcaatataac ttttatttat tgcattcata agtgcattaa tagctgctgt atttatgttt
     3841 tccgatatac ttacaccaaa atagacttca ttattgtgct ctattcctac ataagctacc
     3901 cctcttgaat gtgacccttc ctctaatgca tgttcccagt aatatctaaa tttataatct
     3961 ttcatgccac attgctttag tgcattatta aatgaatcta caggtccatt tcctatacca
     4021 acaatccttt gttcatttcc catataactt attcttgcag tcatctcaat catattagta
     4081 tcattagaat catcattttc atagtttaat tcatccattg attttatttt atacttctta
     4141 actttataag ggctctcaac ttcaatatat tctttcttaa acaaattaaa tattgcttca
     4201 ctactaagtt cttccccaat tctatcagat tccattttga ctacatttcc aaaatcagaa
     4261 tgcatatttt taggtatcat aaatccataa tctgtttcca ttatataaac agctcctcct
     4321 tttccagatt ggctatttat ccttataatt tctttgtatt ctcttccaat atcatgaggg
     4381 tctattggta aatatggaac ttcccagtaa tcattatctc tgtttttcat tgctatcatg
     4441 ccttttctaa tagcatcttg atgtgaacct gaaaaagcac aatgtacaag attacctgca
     4501 tatggatgtc tatcatgtac cataagtttt gtacagtctt catatatacc tattatctta
     4561 tctatattgc taaaatctag ctctgggtct attccttgtg tataaagatt aagtcccaca
     4621 tttactatat ccatattccc agttctttca ccatttccaa atagtgttcc ttcaagtcta
     4681 tcggcaccag ccaataatcc aagttcactt gcagccgtac aagttcctct atcattgtga
     4741 gtatgcaaac ttagtataat agaatcccta caacttatat ttttacaaaa ccattctatt
     4801 tggtctgcat atacgtttgg tgttgccatt tcaactgttg atggaagatt tattattact
     4861 tttttttgag gagttggttt ccaaacatct atcactgcct cacatatttc aagtgcataa
     4921 tccatctctg ttcctgtaaa actctctggt gaatattcaa aagtaaattc tgtttctgga
     4981 tattttttta cttcttcatt aaataatttt gctccttcaa ctgctatatc tattatttct
     5041 tgtttactca tattaaaaac cacatctctt tgaagaacag atgtagagtt atataaatgt
     5101 ataattgctt ttttacatcc ccttattgat tcaaatgttt tttctataag atgagcccta
     5161 gattgtgtaa gtacttgtat agttacatca tctggaatca tattctcatc tattaatttt
     5221 cttaaaaatg tatactctgt atttgatgct gatggaaatc ctacctctat ttctttaaat
     5281 cctgtatcaa taagcatttt gaacattctg actttttcat ttacactcat aggtgttggc
     5341 agtgactgat ttccgtctct taaatcgaca ctacaccaaa ttggagcctt aaatatttca
     5401 ttatctggcc atttacgatt tactatttgc attttatcat attttttata ctttccacac
     5461 ttcatgttat ttcccccttt ttctgttttt tgtattcagt atacctcata tcgacgcaat
     5521 cctgcacaat tcataaggat aaatgtcaat tttatcatta gaataaaaaa aacctcatct
     5581 ctaataaaat tagagacgag gttttgctcg cggtaccact ctatttagct ttctaaaaag
     5641 ctcccttagt aatagccaat acaataatta taatgactat atttcctata acgtggaatt
     5701 actccgatta ttctaagatt taaatcttag aaaacagctc ctagacaagt tcagaactat
     5761 caactactaa atcacaccat cctttagctc tctgaaagtt tcattgttct tactacttct
     5821 attcatcact tttttaatca tttaaatttt ctaaatattt tgatttaata gttattatat
     5881 acacattaaa atgtattgtc aacacaaaaa ataattatct caatttttta tttttgataa
     5941 gcaattctat aaaacatgct aactattgaa aacagtatga tattatgcct taaacttttt
     6001 catcttttca tctatgatat tcaataattt tttttcatct tccaaactca atgcccttaa
     6061 tctaatttta taattagatt cgttagtgta tatatttaat atatttttta ctacttctat
     6121 tttttctatt atcttataat taaacttttg actttgttta gattttatac tgaatataca
     6181 ttgattattt attaatattg aatgattatc gagagtaata tatacttttc cctcatattt
     6241 atttgaaaaa tcacgacctt gtttaaatag aaaaactgct gttattatac ataaaatata
     6301 taatattgtc gttgaatata taaaaattgt agctaaaatt ataagtgcta aaaccatata
     6361 tatagtcatt ccttttttaa aataaccatt gtcataataa ttcatataga taaacttatt
     6421 tttactcaaa ttttttcact cccaagattc taaaaattta tatcaaaaaa ttctataatt
     6481 tcatcttcat gataatttat gactaattca tctggaaaat ctactgcttc taaaactttt
     6541 tctgcatttt caaattctcc tatagaataa catatatgag agtcagtacc aagaattact
     6601 cttgcaccat atgttttaca caatgtcaac atatgactta cattttccca agtacccgtt
     6661 ctatgagagt ttgaacttaa tgatgaatta tttatttcta aaaggatatt tttatcttta
     6721 gcttttttta ctataggttc ataatctaaa ggatacctac tgtcatctgg atgtccaatt
     6781 atttttacct taggtttatc cataactttt aaaatagcat ttgtattttc ttcttttgta
     6841 ccagaagcta tacaaggtct atgcaaactt gcgattatat aatccaaatg ttgcaacata
     6901 tcttcttgaa catctaaatt accattataa tccattatat ttccttctat tcctctaaga
     6961 atctttacgc cttgcacttc acgtggaagc aaatgcaaat tataaaaata gaatggatgt
     7021 ggtcctcctg gcatattagg tccatggtct gatattccta agtattttat attcttctta
     7081 ctcgcctctt cgacattttc cttgaaagta ctatgtgcat gtccacttat tagagtgtga
     7141 caatgtacat ccataataga ctttttcatt aaatttcctc ctaatatttt acataaattc
     7201 gtgaaaatag ctaaagggct atttcaaaac taaagctgtc ataatagaca gtgtaacgtt
     7261 ttaaaacagc cccattttca attattttac acttctaaat ctttaaattt atctttaaat
     7321 aattctccaa tacttcctaa agattcctct acttctaggt attctgttat atcctcttct
     7381 ggctctctag ttgcttcttt tattgaaacc aacattctct tagtctttgg tttaaaatca
     7441 agaatcttaa ctttaacctc atctccaata ttaacaacat tagtaacttt agccactcta
     7501 gtatcactca attcagatat tggtaaatac gcatctacac cttctttaat ttgaacaaaa
     7561 gccccttttt ctattattct aagaacttta gcattgacaa catcatcaat ttgtacttct
     7621 tttgctatta aatcccaagg gtcattattt atgtctttta atgccaaggc aattttttta
     7681 gtctctttat ttacatccaa cacataaact tctacttctt gtccctctga aacaacatct
     7741 tcaacacttt caactcttgt ccaagctaaa ttatttatat gtgctaatcc ttcaatccca
     7801 ccaatatcca caaatgcacc ataaggcatt atctttgtca ccttaccatc tcttttttgt
     7861 ccaacttcaa gagaattaaa taagtcttct ttagctgctt tgatacgagc tttttcttct
     7921 tctctttctt gcttaattct tgctctttca gcttcttttt ctgccttaat ttgagctctt
     7981 ctttcttctc tctctttatt tatacgctct tgtaatacat ctctatgaga tgctacaagt
     8041 ctattttttc taacatccaa ttcttttata taaacttcta agttaagacc aacaaattta
     8101 gatgtatctg taataaattt tgtatcaagt tgagaaattg gtataaaaaa tgtatatgta
     8161 ttgtaattag caaatacacc tttatctata ttcttttcaa catttacagt tattattcta
     8221 tgttcatcat aagccttttg taactcggca aaatcatttc ttttgtcaag ttgtaattta
     8281 gataaagtta ttgtaccatc tttttgagat acttttgtta ttacagcagt tatttcatca
     8341 cctatgtggt atatatcttc tatatattga tttttttcta tgttcaattc acttataggt
     8401 attattccat caaacccata gtctaatcct aattgtactt catcatttct cactgcagtt
     8461 atttttccag ttgtcaattc tccaactttc actttgctga atacttgttc ttgctggtca
     8521 agtaattctt gcattgttaa atcattttcc attataaaca tcctttcctc ttattgatta
     8581 tcatatctgt aaatattata ccattaatat tgcaaatact tacatttttc ttactataat
     8641 ttttaaaata aattcaactt catatattta tacattatag aaaatatctt attaaattaa
     8701 aa
//
LOCUS       DBGJFU010000149         8578 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.152, whole genome shotgun sequence.
ACCESSION   DBGJFU010000149 DBGJFU010000000
VERSION     DBGJFU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.152"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            257..1687
                     /locus_tag="ACOIAS_003269"
     CDS             257..1687
                     /locus_tag="ACOIAS_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2702958.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1790..2893
                     /gene="hisC"
                     /locus_tag="ACOIAS_003270"
     CDS             1790..2893
                     /gene="hisC"
                     /locus_tag="ACOIAS_003270"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGZ2702959.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2926..3144
                     /locus_tag="ACOIAS_003271"
     CDS             2926..3144
                     /locus_tag="ACOIAS_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2702960.1"
                     /translation="MNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAHVEVDES
                     KCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3161..4210
                     /gene="vorB"
                     /locus_tag="ACOIAS_003272"
     CDS             3161..4210
                     /gene="vorB"
                     /locus_tag="ACOIAS_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HGZ2702961.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVIEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALTSKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4212..4952
                     /locus_tag="ACOIAS_003273"
     CDS             4212..4952
                     /locus_tag="ACOIAS_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HGZ2702962.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4955..5494
                     /locus_tag="ACOIAS_003274"
     CDS             4955..5494
                     /locus_tag="ACOIAS_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HGZ2702963.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5618..7717
                     /locus_tag="ACOIAS_003275"
     CDS             5618..7717
                     /locus_tag="ACOIAS_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HGZ2702964.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIENRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7984..8499)
                     /locus_tag="ACOIAS_003276"
     CDS             complement(7984..8499)
                     /locus_tag="ACOIAS_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HGZ2702965.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
BASE COUNT         3264 a         1030 c         1676 g         2608 t
ORIGIN      
        1 tgacatttca aaacaataaa ataataaaaa acaaacctaa attaagtcta aatagctttt
       61 tagttaattt tagtagtaaa aaataaaact taaaaagtgt ttgacaatta tgaattttga
      121 gaatataatt aaattaaaat tatacacata taaaaatata ctcctgaatc aagaatgaga
      181 tatttaagat tcaaaatata tattattata cgtgaataat aaaaaagtaa gaaaatttta
      241 taagggaggg gtagatgtga gtagtcaaga aagcaaacca aaaggaaatc ttatatcaaa
      301 ttttagagat gatatgagag accctaagaa aagagcaata tacacatatt caagttctat
      361 aaacttagcg tacaatttta taggttactt tgtatcaact gttacaaata tttttttaac
      421 tgatgtggca aaacttggtg taattatggc aggtggtatg caaattatac aatctttcat
      481 aaaaattgct gtagctccta taacaggtac agtatttgac aaacaaccat tcaaaaaagg
      541 aaagtattat ccatggctta gatatgcacc agcagcgtta gcattaacat atggaatatt
      601 tttcatagtt ccattaatga aaataagtcc agcattactt ttacctttga tgacagcttt
      661 attattaata gcatcttgtg ctcagcaagt agttttcact ataatttatg ctatttaccc
      721 tgtagttgct aagacaccta gagaccgagt tataggctca actactacga atatatttaa
      781 agaagttggt aaattcttag taggtttttc atatccgtta ttattagttt tatttgcaga
      841 tgtattaggt ggagaaggaa tgggttattt aggtacctac ttattctttg cagcaggatg
      901 ccttttagta ttttggtttt cttcaacaga gataataaaa tctggagcag aagaagaagt
      961 tcttcaaaaa gaagagaagc caaagattaa agcaagtgaa atggcaaaag ctttattcac
     1021 taatgtacca cttatgatgg cattttgttt agaatttctt atatgtataa gatctatagg
     1081 attaggacct ttggctccat attattttaa atatgtaata gaagatgaac gtggattagc
     1141 agtattttta tcaatcatgc ctttggtaag tgtagcattc atgtttttcg caccagtgtt
     1201 tataaaaatc tgtagagagc aaaaactagc ttcaataatt tctttttcta tatgtgcact
     1261 atgccactta ttggttgcat ggactccttg ggggaaaact actttaggag taactatgtt
     1321 gctggcaatt ggtggaggtt tttcaaatgt agtatcaata ataaatctta atttctttgc
     1381 cggttcttgt gattatgggc attggaaatc aggaaaagac ttacctggct taagtatgtc
     1441 actatatcca gttgcaatac aagtaggagt attacttgca actactataa gaacagtttt
     1501 aatgaactca atgggatatc aagctgatat ggtagtcaca gaagcagtta aatcaggttt
     1561 tataaatatg atttcttatt ctatggcaat acctttaata atagctgttg ttatagcaat
     1621 actatatcca gtaagtgaca agaaattaaa ccaaataaga gaagagttaa atcaaagaaa
     1681 tgcttagtta aaagaaagtt aagtagatgg ttaatttaag ctattaaagt aagtagacgc
     1741 aatagcgttt atataaatga aaatacatat acagaggggg cttttaaaaa tgatagaaaa
     1801 attattgaga aataccgtaa aagaattaca tcaatatgtt ccaggtgaac caatagaaaa
     1861 agttaaggaa aaatatggtg taaaagaaat aataaaatta gcttcaaatg aaaatcctct
     1921 aggaccatca ccaaaagcaa tagaagctat gattgaaatg ttaaaacaag gtcaacttta
     1981 tccagagcca gaagctaacg aacttagaag aaaattagct gaaaagctag atttaaaacc
     2041 agaaaatttt attgttgcta atggtgcaga taacgttatc actctaatag gtgaggcttt
     2101 tataaataga ggagatgaag ttatatattg taacccaaca tttccatcat atagaactgc
     2161 aactataaaa aatgaaggga taccagtaga agtaccttta acagaagatt ataagtatga
     2221 tttacaaggt atattagata agattacaga taaaacaaaa ttaatatgtg tatgtaaccc
     2281 taataatcca acaggaacaa ttgtggatga taaagagtta gaagaatttt taaagaaagt
     2341 ccctgaaaat attataacta tattggatga agcatatata gaatttttaa cagttcctaa
     2401 ttatgtggat ggacttaagt atgtaaggga aaattataac gttatagtaa caagaacttt
     2461 ctctaaaata tatggtcttg cagggcttag agtaggatat ggtattgcta aagatgagat
     2521 aataagaact ttatttacag taaaagagcc tttctctgca aatcgtgttg ctataagtgg
     2581 agcaacagca gcacttgatg atgaagagtt tataaaagaa acatatgaat taaatagagt
     2641 tggaatggct tactttaaag aagagtttac aaaaatggga tttgatgttg tagattcaca
     2701 atcaaatttc ctttatgtag atatgaaaac agatatacct aagttatttg aagacttgaa
     2761 aaaaagaggg tttgtaataa gaccaagtgc tacacatgca cgtgtaagta ttggaactat
     2821 ggaagaaaat aagaaatttg tagcagtttt aaaggaaata ttaaatatta aaggagagcc
     2881 aagtattgga taatagatag aaatattatg aaacggggtg tagatatgaa cttaaaaata
     2941 aacgcagaga gatgtaaaag ttgtgaatac tgcgttatat cttgtaaaaa aggagcatta
     3001 aaaatatcaa gtaagataaa caaagaaggt tatgctcatg tagaagttga tgagagtaaa
     3061 tgcatacttt gtggaatttg ctatcaggtg tgtccagata atgtttttga aataattaaa
     3121 gaagaagcat gtcaagaggc ttagaaggag ttgggataat atgaaaaaga tgtggaaggg
     3181 gaaccacgcc attgcagaag cagccctaag aggaggttgt gaattttatg caggatatcc
     3241 aattacacca caaacagagg taattgaatt tttatcacat agaatgtctg aacttggaag
     3301 aacttttata cagtccgaaa atgagatggc tgctatatat atggtatatg gagcatatgc
     3361 aagtggaatg aggtctatga cgagttcttc aggaccagga atatctttaa aacaagaagg
     3421 gatttcttac ttatgtgcaa atcattaccc ttgtgtaatc gtaaatgtac agagatgggg
     3481 gcctggtctt ggctcattgg actcagcaca gacagactat ctaagagata ctagaggggg
     3541 aggaaatgga gattatcatt tgatagttta tgcaccaaac agtattcaag agactgtaga
     3601 tttgatgtat aactcatttg atgtagcgga gaagtacaga gtgcctgtcg aaattttgac
     3661 agaagcagct ttaggtcaaa tgatggaacc agtagaattt ccagagttta agaagagaga
     3721 aggagattta ggctggactt atgatggaag taatagagac catgcaaagg ttgcagataa
     3781 ccaaaagcca actttttgta tggagaaacg tatgagaata actgaaaatg aacaacaatg
     3841 ggaagattat caagtagaag atgcagaata tgtgtttgta gcgttcggta taccatcaag
     3901 aacaactatg aatgctgtta gaagacttag agaacaaggt gaaaaagttg gaataataag
     3961 accaataaca gtatggcctt tcccatataa agcatttgaa aaggtaagta aagatgtaaa
     4021 aggatttatc tcagtagaat caacagacac aggtcaatta gtagaagatg ttgctttaac
     4081 ctcaaagaaa gtatgtaaag aaaatgtacc tgtgtatggt ttatttagtg gaaatcatat
     4141 accaaaaaca ttgcaagtaa tggacactta tagcaaaata aaatctggcg aaatcaagga
     4201 ggtgttctaa tatggctaca gaaaaattaa cacctagcgt tgtttcaata ccaaacaagt
     4261 tttgcccagg ttgtggtcat ggaatagtaa atcgtataat agcagaggta atagaagaac
     4321 atggatatga aaaaaatcat gtattaacat taggagttgg ttgtgtctgc aatatgaatt
     4381 ttagctggaa tggtgataag atgcaaactg cacatggtag agcttcatct acagctatag
     4441 gagttaaagt agcacttcca gatacattag taatgactta ccaaggtgat ggagatgctt
     4501 atgttatagg tctttctgaa actttaaata cagcatatag aaatcataat gtgactgtat
     4561 ttgtaataaa caataataat tttgcaatga ctggtggaca aatgtcatgg accactatgc
     4621 cgggtcaagt cacaacaaca agtgtaaatg gaagagatat aaagactact ggtagtccac
     4681 tgaaagttcc tgagatggtg gcaaacttct ttgatgtagc atatgtagca agaggttcag
     4741 ttcatagccc aaaggaaata attaatctaa aaaaatatat aaaaaatgca atagaagctc
     4801 aattaaatgg agagggatat tccttagttg aaatattagc accttgtcca actaactggg
     4861 gtgtatcact agagaaatct atcaaatgga tggaagaaga aatagtgcca tattatgctc
     4921 ttggcgaatt taagcaaagg gatggtgaat agttatggaa aaagagctag tatttgcagg
     4981 gtttggtggt caaggagttt taactcttgg tctgattgta gctgagtcag cacttgaatt
     5041 ggggaaacaa gtaacttgga tgcctgcata tggtccaact atgagaggtg gaaaagccta
     5101 tagtgtagta aagttttctg atgagagtat aggtgggcct gatatggaag aaatagatgt
     5161 tttagtagca atgaataaac catctttaga ttatataaat ttagttaaag aaggtggaac
     5221 agtagttatt aatacatctg caatagatga gaatgtaaca ttaagagaag atataagtgt
     5281 agttaaaata aattgtcagg aattagctca aaaagtaaac aatccaaaag cagcaaatat
     5341 agtagtactt ggagcattaa ttgctaaaac agggttattt gaaaaagaat tagctttaaa
     5401 aactatgtgt gatttctttg aggaaaaagg aaaaggaaag tttaatgttc aaaatgaagc
     5461 agcattcatg gaaggctata ataacgccat ttgataaatt acccttatct taagtgattt
     5521 atctcaagat ttaataatct ttagtgccaa gagaaacttc atcttgagat aaaattcact
     5581 taaaatcaca actataaaca tataggagcg tgaatttatg agtttggaaa aacttattag
     5641 accaaaaagt attgctatag taggagtgac tgataaactt ggttttggaa gaagtgcagc
     5701 acttagtata gtaaaaagta aggaaacaga tagagtatat tatgttaatc ccaaaagaga
     5761 agaattgttt gggagaaaat gttataagac gatacaagaa gttccagaag tagtagactg
     5821 tgtagtagta tgtacaccta gaaatgtagt tccatctgta ttaaaagatt ctggagagtt
     5881 aggaacaaag gcagcagtgg tatatgctag tggatttgca gaggaaggta cagaggaagg
     5941 tacagattta gaaaatcaac ttatagaaat atcaaataca tacgatatga aaatattggg
     6001 tccaaactgc atggggttgc taaattgtat agataaggtt aatctatggg caggaggttc
     6061 taaatgggat ttagatacta aaaaacctgg tataggaatt gtaggtcaaa gtggatttat
     6121 aactgctgaa attgtttcaa gtgattattt taatatatct tatggttttt caacaggaaa
     6181 tggaaatata gttacactag aagatgcagt agatttcctt gtagatgatg attatgcatc
     6241 agtaatagca atttaccttg aaggtttaaa aaatcctcaa aaatttatag atgctttaaa
     6301 aagggcagca caaaaaagaa agccagtaat aatattgaaa tcaggaaggt cagaaaaagg
     6361 tgctatttct gctgcttctc atacaggaaa tttagctggt tcgagtaaag cttttgaaag
     6421 tatttttgaa aaatatggag taatttctgt ggaaaattta gaacagttta tgtgtttagc
     6481 acaagctttt agtgttttag atggtaattt acctgcaaat tctaattttg cagcaataaa
     6541 cttctcagga ggagaaaata ctatttgtgc agatttagca gaagaaaatg gtgtagaact
     6601 tgcagaaata tcttctgaaa ctaaagaaga aatgaaaaaa tatcttccag gatttgcaac
     6661 acctaaaaat ccactagatg ctacaacagc attattccat gaaaaggata tgatagtagg
     6721 tttattacat acctttgaaa aggatgatag tgttggattt actatggtag gtgcaaatat
     6781 aagagatgaa gaaaatgaaa tgcatgaaac cctttgtaaa gctgtaagtg aagctagaga
     6841 acaaggattg aaaaaacctg tgtttgcagt tcctacactt gaaggaaata gatatttaga
     6901 ttataggact agattagaag ataatcaagt gcctataatg tcatcagttg gaactacatt
     6961 tacttgtttt aataaaatgg ctaagtttat agattatgat tattcaaaaa gaactttaga
     7021 atttaaagct gttaagaaaa gagagtcaaa taatgtagta gcattatcag aattagatag
     7081 taagattgag atgaaaaaat atggagtacc agttccagga caaggtaatg caaaaagtat
     7141 tgatgaatta gatgaaatct taaaatatat taagtatcct gttgttttaa aaataaattc
     7201 ttctgaaata ttgcacaaat ctgatgttgg aggagttaaa ataggaattg agaatagaga
     7261 tgaggcagta gatgcatata atgagatttt aactaatgta aaaaaagcaa aaccagatgc
     7321 taatattgat ggtatattag ttcaagaaat ggtagaaagt ggtatagaaa ttattattgg
     7381 tataactaat gatgaccaat ttggaccaat gttattagtc ggtttaggag gagtatttgt
     7441 agaggtattt aaagatacta cattatatcc attacctata aatcatgatg aagctattat
     7501 gatgcttaaa aaactaaaat catttaagct tttaaatggc tatagaggaa gtgagccttg
     7561 tgatattgat gctcttgcag atatgatggt caagttagga aaatatgctt atgaaaataa
     7621 ggatgaagtc aaggaaatag acttaaatcc agtgtttgta tatcctaaag gaaaaggcgt
     7681 atgtgcagta gatgccttaa tagttaaata taaatagttt ataagttttg gtattcaacc
     7741 ataattaaag caccgactga aaatcacatt ttgtaagtta gatagtgtga ttttcagtca
     7801 ccccaaattt aatttaaaaa tattttaaaa tgaaagtata gttttaagtt taattgtgaa
     7861 atagcactgt ataaacaata aaatactacg tataaaacga ataataaaag gtgttttaat
     7921 gataagaatc aatcttgatt ttcattaaaa caccttcttt attatattaa aaatttttaa
     7981 acgttatatt gtcacaggtg ggtttgttgg agctgcatat gtacgattag caaattcttg
     8041 gtcaacaaag aatagaccac cctctccagc aagtttaaca cgttttatat tgtcttcaca
     8101 atttgtttct tcttctgctt gttcagatat aaaccaaagt aagaattgag aagttctaaa
     8161 gtctgattca gagtttgcta cagctgctaa gttgtttatt aaagctgtaa cctttttttc
     8221 atgagctaac gtacgctcaa gaatatccat tgcagaagta aagtctgaat ctggcattgg
     8281 gatagcatca agctgaacct ttcctcctac attatgtaag tatttgtaaa aaaacatagc
     8341 gtggtctgtc tcttctttat attgaacata aaaccaatta gaaaatcctt taagtccttc
     8401 tgcttcaagg taagaagaca tagaaagata aagatacgca gaatataatt catggttaat
     8461 ctgctcatta agtaattttt ccattttagc tgaaatcatt tttatacctc caaattttaa
     8521 atgctcttat aaaatataat aatattaaat ggaaaaatat tcaataaaag tgtttaaa
//
LOCUS       DBGJFU010000150         8568 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.153, whole genome shotgun sequence.
ACCESSION   DBGJFU010000150 DBGJFU010000000
VERSION     DBGJFU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.153"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            133..1992
                     /locus_tag="ACOIAS_003277"
     CDS             133..1992
                     /locus_tag="ACOIAS_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HGZ2702966.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2284..2883)
                     /locus_tag="ACOIAS_003278"
     CDS             complement(2284..2883)
                     /locus_tag="ACOIAS_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGZ2702967.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2986..3783)
                     /locus_tag="ACOIAS_003279"
     CDS             complement(2986..3783)
                     /locus_tag="ACOIAS_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGZ2702968.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3896..4001)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4093..5079)
                     /locus_tag="ACOIAS_003280"
     CDS             complement(4093..5079)
                     /locus_tag="ACOIAS_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HGZ2702969.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     regulatory      5243..5419
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5563..7293
                     /locus_tag="ACOIAS_003281"
     CDS             5563..7293
                     /locus_tag="ACOIAS_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HGZ2702970.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGRDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7403..7483
                     /locus_tag="ACOIAS_003282"
     tRNA            7403..7483
                     /locus_tag="ACOIAS_003282"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7437..7439,aa:Leu,seq:tag)
     gene            7818..8333
                     /locus_tag="ACOIAS_003283"
     CDS             7818..8333
                     /locus_tag="ACOIAS_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HGZ2702971.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
BASE COUNT         3088 a         1063 c         1310 g         3107 t
ORIGIN      
        1 aactatgtaa aacaaggttt atatattcta attgtataga aaatttattg taaacgcatg
       61 tatattatta gaaaaaatag tttgaaggta ttcaataaac ataaaaaaca atatagggga
      121 taaaggtgtt ttatgaagag atttttgagg agaattatct tagttttatt tattttgttg
      181 atttttataa gttttatttc tattaaatta attcataatg ttggagatta tggaaaattg
      241 attaattatg ttgggattgt tcgtggagct agccaacgtc taactaaact agagatgaat
      301 catcaaccaa atgatgaatt aatagaatat attgatgaaa tcctacagga gttgattaca
      361 gggcatggag attatggttt agtaatcact gattgtaatg aatacaatga ggacttactt
      421 ttattagaaa aaaagtggga agatttaaat attgaaatta aaaaagtaag aatgaaagaa
      481 cagaataacc agttattaag tataagtgaa gagttttttt ctcttgcaaa tgatacagtg
      541 tttaaaatag aaaacttttc taaagaaaaa agtaattact taatgacttt aataataatt
      601 atatctatta taggtatatt agcatgtatc atattgattt tacaatatag taaaaaaatg
      661 gttagactgg aaaaattaaa tgtagattta aaaaatattg catataaaga tgaactaaca
      721 ggagtaaata ctatagaaaa atttaaattg gatgcaaatc aaaatatttg tatacataag
      781 gataagaaat ttgctgtatt ctatattgat tttgaaaact ttaaatacat taatgatata
      841 tttggatatg attatggtga catgatactg aaaagatatg ctaatcttat gatgaatgac
      901 atagggaaat atgaaatatt tgcaagagaa attgcagata gatttgttgc gttaagatgc
      961 tatgtagata aggaagattt agtggtaaga caacgagatg tagacaatga actaattaat
     1021 ataacaaatg aaattaaaaa taaacatagt attactgttg ttagtggaat ttgttgtatt
     1081 gaagatgtaa atgaaaaatt aagtatagat ggtttaataa atagagcaaa ttttgcacaa
     1141 aaaactgtta agaataaacc aggtactaat tatgcttttt ataatgacag tattcgtaaa
     1201 aaaatgattg aagaaaatac tatcaagagt agaatacatg aagctattga aaaaagagaa
     1261 tttatagttt atttacagcc taaagttaat ttacatagtc aaaaaataaa ttgtgcagaa
     1321 gctttagttc gttggcttac tccggataaa ggtattatat cgccagcaat atttattcca
     1381 gtacttgaga agaatttttt tattgcctta gtagataagt atgtttttga ggaggtttgt
     1441 aaatggatta gaaagaggtt agatgaaaat aaaccttttg ttcagatatc agttaatgtt
     1501 tcaagaatac aattttataa cactaaattt gtagaaactt attccaatat acaaaataaa
     1561 tacaggatac ctaaaaatac tatagaaatt gaatttacag agagtgttgc ttttgagaat
     1621 caaaaacatc ttttggaaat tatacatgat ttgcatgaaa atggatttac ttgttcttta
     1681 gatgattttg gaaaaggtta ttcgtcttta agtgttttaa aagatttacc atttgatgtt
     1741 ttaaagcttg attctatgtt tttcaaggct agtttagata aagataaaga gaaaatagta
     1801 ataaaaaata ttgtacatat gttaaaagaa cttaatatca caactgttgc tgagggcatt
     1861 gaatgcaagg aacaagttga atttcttagg gatattgggt gtgatttagt tcagggattt
     1921 gtattttata aaccaatgcc aatattagaa tttgaaaaaa tattggataa agagtttgtt
     1981 tataatagct aaaatgtact ttataaaata tgaactaatt aagtaaaatt attttgtact
     2041 gaataaattt ttactttatt tacagaataa tgattattta cagaaaagcg cgaaaagtgt
     2101 attcctttaa gtgtgggatg gatagcacaa atagaggaga aaaaatattg gccatataag
     2161 aatgcataat aatataaata tttgaaaggt cagcattata taaaaattaa aaagaaaata
     2221 aataattttt ctataaaaaa gagactatga gaaaatttac tcatagtctc ctaaataatg
     2281 tatttatttt cttcctgcaa cccattttaa tccccaatga aattcagtat ccatttctct
     2341 atgacctttg aagtatttaa ctactttttc aacactgaat gtctcatcat ttacattttc
     2401 tatcaatgct attgaacaca tattatagcc atttttatgt tcatcaagtt tgacaactaa
     2461 gtctgaatct tgtttttgtt tgattgttac aattccatca gcttcagacc agttagctac
     2521 accttcgtat ataaaagcat aaataagtat tctttttata tcttttattt tattaccatt
     2581 tattcttaaa ttttctcctt gtgtattact tcctgttctg tcatctccat ctaattgagc
     2641 aaaaggtgga ttgtctaaac taccaaaggc atttcctaat gcttgaacag ctccttttac
     2701 accatttttc atctcaaata ggcaacccaa atctaaatct atattactgc ttcttaaaga
     2761 gccaaaaaaa cccttttttt gtactttttg attccaattt aaattaacta agatttcacc
     2821 taaatcgcta ttttcctttt ttgtaaggtt tattttttgt ccctttttta attctatagc
     2881 catagtaatc ttcctccaca aacatagatt tatttaaatg gtagcactta ataagtcaat
     2941 attcaacaag attagaataa taatattaaa attatactaa atatactaaa aagtaggaat
     3001 cacaatacct ttgtattttt cttcaattaa gcttcttgct ttatctgatg taagaacatc
     3061 ttgtagaact ttaatctttt cactgtcttt atcttcagtt ctaaatgcta aaagatttgc
     3121 atactcgtca tttataggag ctgtataaat tccgtttttc ttggcatcaa gaccagcagg
     3181 tacagcataa gtagtatcta taaatgcagc agtcacatct ggaagagaag atggaatttg
     3241 agcaatatct aactctttga attgtattcc ctttggattt tctattatgt ccttaggact
     3301 aactaatcca tcccctttag gtaacgttaa aagtccaata ctttcaaggt atctaaggtt
     3361 cttagaacaa gaagatgggt tattgcttat tgcaattgta tctccttttt taaattcatc
     3421 aacagatttt atcttataag agtaaagtat tgctgggcat acatatagtt ttttaccagc
     3481 cattattttg taacctttgg ccttaacagc ttcttttaga taaggttcat gttgaaatag
     3541 atttgcatca atttcaccat tatttaaagc ttcgtttgga agtatgtaat catcaaagtt
     3601 tttaacttct aaagtatatc ctttttcttt aataagtggt tttaattctt ctaataactc
     3661 tcctcctgga actaaagtag caccaacaac aattttttta tcatctttag ctttagaaca
     3721 tccaaccaca gctaaagtta atactagaca taataacaaa cataataatt ttttgaattt
     3781 catataaatt ccctcctgaa tataattgat tagttaaaaa taaaatacca ttctttatca
     3841 cactgcaaaa taaaaataag cctgagttta aactcaggct ttaaaagcta atttagactc
     3901 atctttcagt ataatactgc aggatttagc accaatgtat ataaaaatat actggttgct
     3961 gggtttcata gggccagtcc cttcaccact ctggataaga agtatcaatt tttaattatg
     4021 ttataaatat tatcatgatt ttttacgtct gtaaacataa ttttataaat attttaaaat
     4081 ctttaatgta ttttatttaa cacctaaaat tccagaaact agaatatcag tatcagcacc
     4141 taagaaatag tcatttattt caatagaatc cagagtattt ctcaaagcat tttcttcatg
     4201 ccttatacca ataagtttat tttctacaaa tgcaacatca cattttccaa aaaaatctcc
     4261 aaaaaatttt atactagtta ttatgccttt atcaacattt aaattaaact caacatttcc
     4321 accagaatat tttaattcat ttgaaagagt atattttggt gaattaccaa aattccaatc
     4381 ccaagtttcg tattttgatt tagtaagctt ttctatttgt tttatatcat gctcactcaa
     4441 acaataatta gtattatcag tatttgtttt agaaagataa tccattatag catctttaaa
     4501 ttctaatatg tccatttttg aatgtaaatg ttcacttata ttggttacac gagattttac
     4561 agattttatt ccttttcctt caaattttat aggtttaacc tttaatgctt ttgagaggtc
     4621 atttatttca gatgaaaata aaagagtacc atgatgcatt actttgtttt tgtagttgta
     4681 ttgagcattt ccagaaaatt tcttgccatc tataagaagg tcatttcttc cagaaaattc
     4741 ggcattgaca tctagtgttt taagtaaatc tattatgggc tgagtgaatc ttctgaaatc
     4801 actaaaatta ttattgttac aagctataaa tgtaaaattt atatttccca aatcatgaaa
     4861 tacagctcca ccaccagact gtctcctaac gacaggtata gaatattctt ttatatattc
     4921 taaatttatt tcagaaagtg tgttttggtt ttttccaaca attatagaag gttcattcct
     4981 ccataaaata aataagtctt catcaaaatt ttttagaaga tattcttcca ttgctaaatt
     5041 aaaataaggg tttgtttttt cattataaat taataacatt aaagtagttc ctccatagaa
     5101 ttgataaaat ttttattatg aatacaatta attttataat tcgacaataa tataaaaact
     5161 cctttaaatt agaaatttta aaaactacct tgcaaagttt aaataaataa tatatactca
     5221 aaatatgttt aataaaagtg aatagaaggg tagaggcgca ataatctatc agtactaata
     5281 aggagttaag cacagagatt tattaggaaa ggagatattg ccgaagtttt ataatcgatg
     5341 ctttaagatt atattgctgg gggtacatag aaaatatgta caactgtcac aggtttattt
     5401 gtggtgagct atcatttaag gaattatatt atatttattt ttactattgt tgatatattg
     5461 taatgtttaa gccttgagtg tttaccactc aaggcttttt tagttttatt gtaacataat
     5521 accctgtaca gttcacaaaa ttaaaaggag agggagattt atatgaggaa caaaaaaatt
     5581 aatattattt ttctgacgac aatcatgttc ataatgtcaa cagtaatggt ttttgcagaa
     5641 gaagatatag acactattgc actagccaat gcagaaaaat ttgggatttt aactttaata
     5701 ccaccattag ttgctataat acttgcattt ataactaaaa acgtaataat ttctttactt
     5761 ataggtattc tttctggtag ttttataatt aaagctagtg gtattaatgt ttttgcgaca
     5821 tttatacagg catttttaga tttagtagac agagctttag tatctttagc agacccttgg
     5881 aatgcaggga taatactaca ggttttggca attggaggag taataaatct tgttgctaaa
     5941 atgggaggag ctaaagctat agctgaagca cttgctaaaa gagcaaagtc agcaaaaggg
     6001 acacagctta taacatggtt tttggggctt ttagtattct ttgatgatta tgccaattca
     6061 ctaatagtag gtcctatgat gagacctgtt gcagataaaa tgaaaatatc gagagaaaag
     6121 ctagcattta taatagatgc cacagcagca ccagttgctg gtttagctat aatatctaca
     6181 tggataggtc ttgaggttgg tttaatacat gatgcttttg aaagtataag tatagatgta
     6241 gatgcatttg gtatattttt aaatacaata ccatttagat tttacaatat attaatatta
     6301 gctttcattg taatatcagc attattatta aaagaatttg gtccaatgag aaaagctgag
     6361 ataaaatcta gaagtagaaa aattagtata gaccttgatg aaggcgtaga agagttggat
     6421 gatttagctc caaaaaatgg agtaaaattg agtgtatgga atgctataat cccaatagga
     6481 acattgataa tagttgcatt agcatcattt tattatagtg gatatacatc tataatggga
     6541 agagacgata aggctctgat acaactattt acaaattctc catattcttt tgaagcaata
     6601 aaagaagcat ttagtgcatc tgatgcatct agagcattat tccagtctgc acttgttgca
     6661 agtttagttg ctattataat ggctgtagta aaaaagattt ttacaatatc agaagcaatt
     6721 gatgtttgga tagatggaat gaaaagttta gtaatcactg gtgtaatatt aatacttgca
     6781 tggtcattaa gttcagtaat aaaagagtta ggtacagcta aatttttaat acacctatta
     6841 tcaggttcac taccaccatt cttattacca agtttaatat ttggtttagg agcaataata
     6901 tcttttgcta ctggaacagc ttatggaaca atgggtatac ttatgccact tgcaattcca
     6961 ttagcatact cattaaatcc tgatatgtct tatgtaatag taagtactag tgcagtttta
     7021 acaggtgcaa tatttggaga ccattgttct ccaatttcag atacaacaat actttcttca
     7081 atgggagctg gctgtaatca tatagaccat gttaacactc aaatgccata tgctatattt
     7141 acagcagtaa tcactattgt atttgggtat ataccagctg gacttggatt gccaatatat
     7201 atagtattac cagtggcaat agctgctata tttgtaggta ttcaaataat tggtaagaaa
     7261 gttgatgaag cggaaataga acttgtagaa taaagatata aataagtttc accttttatt
     7321 atcactattt tgttagtttt tagataattt taaaaagtta ttgaaaataa tggcgaaata
     7381 tagtaaaata aaagagtgat aagcggatgt ggcggaattg gcagacgcac tagacttagg
     7441 atctagcgcc attggcgtgg gggttcgact cccttcatcc gcactttaca gtacttaatt
     7501 gtactgaaaa aaactggggt tgctggcttt cggagtgggc tatcccgttt ttattttttt
     7561 gtatatataa tagattggca ctttagtata aggtgctttt tttataggca aaaacagtac
     7621 atttatttct gttttattat cattcatcta ttagaatata ctttagttaa gaatgtgtta
     7681 ataagcagtg ttcacattct tgataaaatg tggaataata tgtattttta ctaagacgga
     7741 aatgaaataa ttaaaccatt aaataatatt ataagatgat gaataagtag ataaatcaaa
     7801 ttttgtagga ggatattatg gaaaaatata tatggttgtt tccattactt tttatttttc
     7861 atgatatgga agagataata ggttttggaa tatggcttaa aaaaaataaa tcaatgcttg
     7921 ataagaagta tccatttatc agtaaagttt atgaaaatta tagtactgaa ggcatggcat
     7981 ttgctgtgtt tgaagaattt attttatgca ttattttctg tatacttaca gttataacag
     8041 agaatcagta tgtatatcta ctatggttag gtagttttat agcatatacc ttacatttgg
     8101 taattcatat agggcagtca atcataataa gaaaatatat accttcactt attaccagta
     8161 tcatatgctt accaataagc atttggtgta taagtaagag tatttatata gttgattgtg
     8221 aaatgagtac aacaattctg tacagtataa ttggcattat aattgtagca ttgaatttaa
     8281 agtttgcaca gtcgctgatt ggaaaattta caaagtggat gtcaaacata taaattttag
     8341 tttattggac agttcaagga atcagaaatt ggaattttat agtattaata ttttatggta
     8401 ttaatataat gtataaaatt ttatagcaat gtatttcata agtataaagt gactagaatt
     8461 ttaagaaata tacaaattct ataaaaacta aataatttca agtatatttt aaaaatctta
     8521 tttttcacaa gtttaaaatt gtaaacgatt tcatcaagtc tatgaata
//
LOCUS       DBGJFU010000151         8368 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.154, whole genome shotgun sequence.
ACCESSION   DBGJFU010000151 DBGJFU010000000
VERSION     DBGJFU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.154"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            39..164
                     /locus_tag="ACOIAS_003284"
                     /pseudo
     CDS             39..164
                     /locus_tag="ACOIAS_003284"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
     gene            219..485
                     /locus_tag="ACOIAS_003285"
     CDS             219..485
                     /locus_tag="ACOIAS_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HGZ2702972.1"
                     /translation="MAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDYLIKVL
                     AEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            534..1118
                     /locus_tag="ACOIAS_003286"
     CDS             534..1118
                     /locus_tag="ACOIAS_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HGZ2702973.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1123..1446)
                     /locus_tag="ACOIAS_003287"
     CDS             complement(1123..1446)
                     /locus_tag="ACOIAS_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2702974.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1598..2116
                     /locus_tag="ACOIAS_003288"
     CDS             1598..2116
                     /locus_tag="ACOIAS_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2702975.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2292..2960)
                     /locus_tag="ACOIAS_003289"
     CDS             complement(2292..2960)
                     /locus_tag="ACOIAS_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HGZ2702976.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3075..4490
                     /locus_tag="ACOIAS_003290"
     CDS             3075..4490
                     /locus_tag="ACOIAS_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGZ2702977.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4689..5171
                     /locus_tag="ACOIAS_003291"
     CDS             4689..5171
                     /locus_tag="ACOIAS_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2702978.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            5168..5752
                     /gene="clpP"
                     /locus_tag="ACOIAS_003292"
     CDS             5168..5752
                     /gene="clpP"
                     /locus_tag="ACOIAS_003292"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGZ2702979.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6064..7140
                     /locus_tag="ACOIAS_003293"
     CDS             6064..7140
                     /locus_tag="ACOIAS_003293"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2702980.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7207..>8368
                     /gene="bclA3"
                     /locus_tag="ACOIAS_003294"
     CDS             7207..>8368
                     /gene="bclA3"
                     /locus_tag="ACOIAS_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HGZ2702981.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGA"
BASE COUNT         3182 a         1072 c         1535 g         2579 t
ORIGIN      
        1 gtattaaata attaaggagg tgagtagctc tggatgaaat agatttaaaa ataattaatt
       61 ctctaaaaaa gaatagtagg gcttcaacct ctgaaataag cagacaaatt aatttatcag
      121 taccagcagt ctcagagcgt ataaggaaaa ttgagcagtc aaatttaatt caaaagtaca
      181 ctattcaaat taatcgggaa aaaacaaaat ataagctttt ggcttttata tttatcaata
      241 ctgatttatc taaaagtatg aaagaattta agaaaactat attggaatat gatactgtat
      301 tagaatgtca tcatatttca ggagagtatg attacttaat aaaagtatta gcagaagata
      361 caagtaatct tgaagagttt atatcagtga ctttgaagga aattgggggg attcaaaaag
      421 ctaatacaac cattgtttta tctacaatta aagaagaatt taatacaata aaagatattt
      481 tttaaataat ttactagtaa agtgttaatt atattttagg aggaaaatag aatatgaaag
      541 ttttagtttt tttagcaaaa ggttttgaaa ctatggaatt tagtgtattt gtagatgtta
      601 tgggatgggc tcgtaacgat tatgggcatg atattgatgt agtgacttgt ggatttaaaa
      661 aacaagttat gagcacattt aatatacaag tattagttga taagacgata gaagaagtat
      721 gtgttgatga ttatgatgct ctagctattc ctggaggatt tgaagaattt ggattttatg
      781 atgaagcata tgattcatct tttttaaatc taataaggga gtttaattca aaagaaaaaa
      841 taatcgcttc tatttgtgtg gcagcattgc cagttggaaa aagtggtgtt ttaaaaaatc
      901 gtaaagctac tacatatcat ttgaaaaatg gtaaaagaca aagacagtta agtgagtttg
      961 atgtaaatgt agttaatgaa ccaattgttg tagataaaaa tattataacc tcatattgtc
     1021 cagaaacagc tccacatgta gcttttaagc ttttagaaat gttgacatct aaagaacaaa
     1081 tggatgaagt taaattagca atgggattta aactgtaatg gtttatagat tttctaagta
     1141 atcagaaccc cagtcacaca tggaatctaa aattggaata atactctttc caaaatcaga
     1201 tagcgagtat tctacttttg gaggaacaac aggataaacc actcttataa ttaaaccatc
     1261 tgattctaat tcacgtaatt gttgagttaa catcttaggc gttgctttag gaattaattt
     1321 ttttaattca ttgaacctca atatagtgtc tttaagatgc caaagtataa gtgtcttata
     1381 tttaccacca atcaatgcaa gtgttgcttc tattgggcaa ctgtaattat ctaaacattt
     1441 tttcattttt gggctccttt atttatttta aagtatcttt ttaggtacta tattacaaaa
     1501 aagtgcatta ttgtaatata gtaccatagt gataaaatta tagcataggt taaataaatc
     1561 atcaacttag cctaataaaa caataggggg taaataaatg aatttcgttg aacttgcaaa
     1621 aaaaagatat tcttgccgta attatcaaga tagaaaggtt gaaaaagaaa aacttgagaa
     1681 ggttttagat gttgcaagaa tagctccaac tggtggtaat cgtcaacctc aaagattgat
     1741 tgttattcag gaaaaagaag gtataaataa actttctaag gctgccaata tatatgatgc
     1801 gccattagca attcttgtat gtggagataa agacaaagtt tggacaagac cttttgatgg
     1861 gaaacaactt acagatattg acacaagtat agttacagac catatgatgt tacaggcaac
     1921 tgaacttgga ttggcaagtg tttgggtgtg ctattttaat ccagatataa ttagagaaga
     1981 gtttagtttg ccagataatc tagaaccaat aaatatatta ttgatgggat atgaatctaa
     2041 gataccagag agcccagaga gacatgaaaa aactcgtgtt cctttaagtg aaattgtttc
     2101 ttatgaaact ctttaattat aaaattaagt tattcatagg ataaatgtaa taaagtgttt
     2161 gttataaaca aaattcaaaa taaataatat ctacatataa ttaattttag tgaagtatat
     2221 ctagtttaac tagaatgtat acttcactaa tttatttatt caaaatattt atgaggattt
     2281 taatatatta actaaaatat atcaattgaa ttaactttta aatctggaaa agcacttgaa
     2341 atatagtgtt ttatatcatt taaatggtct tgaatttgct tttctgatat tttatcaggt
     2401 ccatatacaa aatacataat atttttagta tcttttgtta tagctgttcg attatcaggg
     2461 ccattaagaa tactagataa tataccaata ttatctgata aaaataaatc atcttttgtt
     2521 aaaatatgtt ctttaccacc tataccataa aaatgttcac ttccatttgc taattctatt
     2581 ttcaaaggta attccatttt atcaaggtca tgacctgctg ttagaagaag atttttcaat
     2641 tctgctaaaa acattgactc aattgttaca cctacatctg ggataccttt tgattttaat
     2701 agaatagatt ctaattgaag caatacaggg taagttttct tgaatttttt ataatacttt
     2761 atataacttg aaacaggctc tgttttaatt ttttctttgc gattgtattc tgggtgttgt
     2821 tctataatat ttttaattgt agagttttta agaactaaaa aattttcttt attaggtttt
     2881 gaatgtgtaa tatttataac cataactcca aatttaatat tgggataaat ttctttaact
     2941 ttatgtgata cctctaacat aatatagact cctttttatt ttatatttta tgatataatc
     3001 ttagcaaatt aaaagtggat tttatagttc catttttata gtaattttat agaaccactt
     3061 ttggaggtat tagaatgtgg attacgatta atagaaatag taatatatca ttatcaagac
     3121 aaatttattg tgagataaaa gatatgattc tggatggaag cttaaagttt gagcaaaaac
     3181 tgccatcatc taggacttta tcaaaagaac tatctgtatc tcgtaataca gttttagatg
     3241 catataatca attaattgca gaaggatatt tggaaacaag gaatggatat ggaacaattg
     3301 tagctaaagg aatatcaggg cataaagttg attttaagtt aaatgatact tttagagatg
     3361 aaaaagattt acatactaat aaacaaaaaa attatataga ttttcgttca ggagttccag
     3421 aattaaaact atttccacgc aaagaacttt caaagctata taaacaaata ctgtatgatt
     3481 tatcagattc ggatttcaga tatggaagta cagctggtgt atgggtttta agagtggaaa
     3541 tatctaagta tttatttcgt actagaggaa ttagatgtaa tcccaaaaac attatgattg
     3601 tttctggttc aacacaaggt ttatctttaa tttcacagct tttatacaaa agtaataaaa
     3661 aggttgctgt agaagaccct attcataaag gcttattaaa ggttatttca tctattggat
     3721 attcagttgt tggaattaaa gtagatgata agggaatgaa taccaactta ataaaatcaa
     3781 taaaggggat ttcttttgtt tatactacac cttctcatca atacccatta ggagggattt
     3841 tacctattca aagaagacta gagcttatta aatgtgcaac agagaatgat tactatataa
     3901 ttgaagatga ttatgatagt gaatttagat atgagggtca accaattagt tctctttatg
     3961 aacttagccc aaataaagta atttacatag gttcttttag taaaatatta tctccagcta
     4021 ttcgtgttgg atttatgtta ttaccagaca atattttaga aagctataaa accttaaaaa
     4081 tgtactctga tgttcataca gaaacaatat tgcaacatgt attagctgaa tttattggaa
     4141 atggaggttt agagaagtac atatttagaa tgaagaagtt gtacaataga aaacgtaagt
     4201 acctcataaa agttttaaac acggaattta aaaatgaatt taaaataaaa ggtgaagcag
     4261 cagggcttca tatagttgct catttctata atgttaaatt tacggaaagt ttaattcata
     4321 aaatttttga agcaggtata aaagtttatc ctatacaaca atatgcaatt gagaatatat
     4381 tagaatatga ctatgaaatt atactaggtt atgctcatct tagttttgat gaaattgaaa
     4441 aaggtgtaaa aatactcagc aaaataatac atatgaatca aggtacatag ataaaacatg
     4501 tagcaatcta catataatag atgtaattaa gtttaaactt taaaagaaat atatattaat
     4561 atttttgtat aaaaatacct gatatttttt tatcgatact ttattgctat acactaaatt
     4621 aagatagcaa tttatattaa gaaaaaactt tttgcatagg ttataatact taaaaggaga
     4681 ggatagagtt gaaaacaaat acagatgtta ttatggagat aattgattat atagaagaaa
     4741 atcttacaga aaatctaaat ttagataaaa tagcttatat tgctaattat tcaaaatacc
     4801 atttacacag aatgtttaca aatgttgtag gatgtactat ccatcaatac atacagagga
     4861 ggagacttac tgaagcagct agacaactta tatttacaga caaatatatt atagacatag
     4921 ctattacagt aggatattct acacatcaat cttttacact agcatttaga aatttatata
     4981 aagaatctcc acaagtatat agaaataaag atgaatttta tcctattcaa cagagatttg
     5041 ttttaaatct aaatcatcaa attcaaagtg aaaaatgtat ttcttatgca aatatgaatt
     5101 ataaacaaag aagagatgaa atcaattctt atcttaactt taatattaat tgtgaggtga
     5161 aagtagcatg agtttagtgc cacatgtaat tgaacaaaca ggtcaagggg aaaggtctta
     5221 tgatatatat tcaaaattat taaaagatag aattatattt ataggagaag aaattaatga
     5281 tgctattgca agtttagtag tagctcaatt actgttttta gaatcagaag acccagattc
     5341 agatataatt atatatataa atagccctgg tggctctgct acatcaggat ttgctatata
     5401 tgatactatg aattatgtca gatgtgatgt atcgactatt tgtattggta tggctgcaag
     5461 catgagtgct tttttacttg caggaggaac acatggaaaa agatgtgctt tacctaattc
     5521 agagattatg attcatcagc caatgggagg agctaaaggt caagcaacag atgtaaaaat
     5581 agctgtagat aatatattga aaataaaaga acgattagat aaaattcttt ctgaaaatac
     5641 aggtaaatca atagaagata ttaggagaga tacagataga gataatttta tgactgcact
     5701 agaggctaaa gagtacgggc tgattgatta tataatgaat aggaatgaat aaagtatcaa
     5761 agtatcatat taaaatgtct gttttacaaa tatatatata atattaaata agagtgtctc
     5821 aaaatgaact ttttagttta tgggacactc ttttttgtat gaaattaaat atggttttat
     5881 catttcaaca attattacca aatttcaagt ttaataaatt ctactataaa aaatacataa
     5941 aagataaggt tctttagtaa ctttctgtcg tcaaaagttt ttataaattt aataacaaaa
     6001 caagagaaaa taccatatat tacttatgtc tgcaatatat agaagacaaa ggagttgtac
     6061 attatgatta caataagttt gtgcatgatt gttaagaatg aggaagaagt tataggcaga
     6121 tgcttagaat gtgtaaagga tatagtagac gaaattataa ttgtagatac aggttctaca
     6181 gattccacaa aagaaatagt ctcaaaatat acagacatgg tgtacgattt cgaatggata
     6241 gatgatttct ctgctgcaag aaatttttca ttttctaaag cttctaaaga ctatactatg
     6301 tggcttgatg cagatgacat aattttagaa gctgatagag aaaaattatt aaagattaaa
     6361 gaaagcttgg atacttccat tgatattgtt atggcaaaat acaatgtatc ctttgatgaa
     6421 aatggtaacc caactctctc gtattataga gaacgattgt ttaaaagaag ttctaatcat
     6481 aaatgggttg accctattca tgaagtagta cctctttttg gagaggtatt ttattctgac
     6541 attgcaatat ctcataaaaa gcttcataga caggaccctc taagaaatct tagaattttt
     6601 gaaaaaatga tttcagaagg aaaaacattg gagccaagac accaattcta ttattctaga
     6661 gagttgtact ataatgctag gtataaagaa gcaatagaag gttttactaa gtttttagac
     6721 tcatcaagag gatggattga ggattgtatc agtgcttgca gagatttagc cacatgttat
     6781 tacttaatta atgatgaaaa aagtgcctta tattcattgt ttagaagttt cgaatttgat
     6841 gagccaagag cagaaatttg ttgtgacata ggaaagcata tgtttgatag acaaaaatat
     6901 aaagaagcca ttttttggta taaggtagca ttaacaagag ataagaatga tactaatgga
     6961 ggttttaagt caaatgattg ttatggatat ataccatcta tacaattaag tgtctgttat
     7021 gacaggttag gagaatcaga taaagctatt tattatcatg aaaaaacaaa agaaatcaaa
     7081 cctaacgata gtgctgtttt gcacaatgaa aattatttta gtaaatttaa aaatagttga
     7141 taggtcacaa tcatactaaa tttgacatat tatatataga gcttaaaagc tcaaatatat
     7201 caggaggtgc ttttaataat gagtagaaat aaatattttg gaccatttga tgataatgat
     7261 tacaacaatg gctatgataa atatgatgat tgtaacaatg gtcgtgatga ttataatagc
     7321 tgtgattgcc atcattgctg tccaccatca tgtgtaggtc caacaggccc aatgggtcca
     7381 agaggtagaa ccggcccaac aggtccaacg ggtccaacag gtccaggagt aggggcaaca
     7441 ggcccaacag gaccaaccgg tccgactggt ccaacaggaa atacagggaa tacaggagca
     7501 acaggattaa gaggtccaac aggagcaaca ggggcaacag gcccaacagg agcgacagga
     7561 gctatagggt ttggagtaac aggtccaaca ggcccaacag gagcgacagg agcaacagga
     7621 gcagatggag taacaggtcc aacaggtcca acgggagcaa caggagcaga tggaataaca
     7681 ggtccaacag gagcaacagg ggcaacagga tttggagtaa caggtccaac aggcccaaca
     7741 ggagcaacag gagtaggagt aacaggagca acaggattaa taggtccaac aggagcgaca
     7801 ggaacacctg gagcaacagg tccaacaggg gcaataggag caacaggaat aggaataaca
     7861 ggtccaacag gagcaacagg agcaacaggg gcagatggag caacaggagt aacaggccca
     7921 acaggcccaa caggggcaac aggagcagat ggagtaacag gcccaacagg agcaacagga
     7981 gcaacaggaa taggaataac aggcccaaca ggtccaacag gagcaacagg aatagggata
     8041 acaggggcaa cgggattaat aggtccaaca ggggcaacag gaacacctgg agcaacaggt
     8101 ccaacaggag caacaggccc aacaggagta ggagtaacag gagcaacagg agcaacaggg
     8161 gcaacaggag cagacggagc aacaggagta acaggtccaa caggagcaac aggggcaaca
     8221 ggagcaaatg gattagtagg cccaacagga gccacaggag cagcaggaac acctggagca
     8281 acaggtccaa caggagcaac aggcccaaca ggagtaggaa taacaggagc aacaggggca
     8341 acaggagcga caggtccaac aggagcag
//
LOCUS       DBGJFU010000152         8282 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.155, whole genome shotgun sequence.
ACCESSION   DBGJFU010000152 DBGJFU010000000
VERSION     DBGJFU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.155"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            99..1445
                     /gene="rpoN"
                     /locus_tag="ACOIAS_003295"
     CDS             99..1445
                     /gene="rpoN"
                     /locus_tag="ACOIAS_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGZ2702982.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            2241..3263
                     /locus_tag="ACOIAS_003296"
     CDS             2241..3263
                     /locus_tag="ACOIAS_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGZ2702983.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            3323..4330
                     /gene="gap"
                     /locus_tag="ACOIAS_003297"
     CDS             3323..4330
                     /gene="gap"
                     /locus_tag="ACOIAS_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGZ2702984.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            4552..5754
                     /gene="pgk"
                     /locus_tag="ACOIAS_003298"
     CDS             4552..5754
                     /gene="pgk"
                     /locus_tag="ACOIAS_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGZ2702985.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            5780..6523
                     /gene="tpiA"
                     /locus_tag="ACOIAS_003299"
     CDS             5780..6523
                     /gene="tpiA"
                     /locus_tag="ACOIAS_003299"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGZ2702986.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            6535..8064
                     /gene="gpmI"
                     /locus_tag="ACOIAS_003300"
     CDS             6535..8064
                     /gene="gpmI"
                     /locus_tag="ACOIAS_003300"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGZ2702987.1"
                     /translation="MKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTLI
                     NASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMDN
                     AKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALEY
                     AKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAIQ
                     NSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDGF
                     KREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAHV
                     TFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVLN
                     FANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPET
                     GKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLIS
                     K"
BASE COUNT         3452 a          895 c         1603 g         2332 t
ORIGIN      
        1 cccttttgca taaggtatat tttaaatgat agaatataca tatacaggag ggtttttctt
       61 ttataaaata agtttaataa aattgagagg tgagcagaat gaattttaat aatagtctag
      121 aattaactca gtctcagaag ctaataatga ctacacaact taagcaatca ttatcaatat
      181 taaatatgag taagttagag ttagaagaag agataaaaag agaagcagag aataacccat
      241 tagttgaagt cgagaaaagt ggggagataa attgggaaga gtatataaaa gacatggata
      301 ggtcaagacg tttagataga acagagataa gctataatcc tgataatgaa gttaatctag
      361 aaaatttagt taaatattcg tcaaatttat atgaagattt aaaatttcag ataagcctgt
      421 ataaacttac agacaaagaa ttggaagtct gtgagtatat aattgatagt ttagatgagg
      481 atggatattt aagaactgag gaaaaagtaa ttgttgatac cttaaagata gatgaagaac
      541 tttttgaaaa atgtttaatt agtgttcagc agttggaacc tagtggagta ggggcaagaa
      601 gtctttcaga atgtctgatg atacaaatgg ctagtttagg aatatataat gaaattttag
      661 aggaaatcgt tacaaaagac ttaaatttaa ttgcaaataa taaatacaaa gagataagta
      721 aaaagtacaa tatgtcatta caaaaatgtg ttgaactaat aaacataatt aagacattag
      781 acccaaaacc aggaagaact tgttcagtag agaaaagtgt atacattcaa cctgatgtaa
      841 cagtagaaaa aattgatgat gaatttattg tatatttaaa tgaaaaagat tcttatcaaa
      901 taagaatcaa tagttattat aaagacatat taaaaaattc acagtctgat gaaagtgcaa
      961 aagaatttat aaaagaaaga ctaaattcag caacaggact tatgaaaaat atagagagta
     1021 ggaaaactac agtacttaag attgcagaag agatagtaaa agctcaagat gaatttttga
     1081 gaaaagggac aaaatatata aaacctttga aaatgaaaga catagcagaa aaattagaat
     1141 ttcatgaatc tactataagt agaggtgtaa atggtaaata tatgttaaca ccttttggtg
     1201 tatatgaatt tagatacttt ttcagtagtg ccattgagac tgagaataat gaaatggcat
     1261 ctagtacaag cataaaaaaa attataaagg aaacaataaa agacgaaaat aaaaagaaac
     1321 ctttaagtga tgaccatata tctaagttac taaaagaaaa aggaatcaat gttgcaagaa
     1381 gaacagttgc caaatatcgt gaagaattag gaatcccatc ttcaagtaag agaaaagaat
     1441 actaataagt aaaagacaaa tatcagtatg gtaaggggaa aaggctaatt aaaagatgaa
     1501 tgtagattgg gctaaaaatt ttatgaagta aaataataga atatggaaaa gtattaaaat
     1561 gctataagat attaaaaaac tggggaacat taaaacatta agatagcaaa gaacataaaa
     1621 aactaaagaa gattaaaaca ttaaagaaca ttagagcttt gaggaatatt agaactttaa
     1681 agaatattaa aatttataaa atataaaaat actaaaaaga attgaaaaac tggggaacat
     1741 taaaatattg taaaatgcta aaaaactaga aaacataatt aaataagtgc ttataaagaa
     1801 tgattaaatt aaataagtgt ttgtaaagaa taattagata ttagaaaaag aagaatacct
     1861 agataaataa actattatta aatttaataa tcataaaagg aaaacacagg gtataaataa
     1921 aaatcttaac tagataaaag taaacttagt tgtagataaa aataactaga aataaaagta
     1981 actagaaata aaattattta tagtaataaa tattagaatg aaaatataaa aaaatatata
     2041 taaaaagcct tatataaaaa gaaaaaatac tgtgatttga aaatggtgga gaaatatttt
     2101 ataaaataaa aaaataaaaa agtttttgaa aaactattga aatctatacg caaatcctat
     2161 aaaataaaaa tatcttgtgg gacttaaata aacactaggg acgaaaaaag tcccgaaagg
     2221 aaagcaataa ggagtatgtt atgaagaact tattagaaat tcaaaagaaa ttgattccac
     2281 aggcaattga acttatggaa agaagatact ctattttaag acaaatatct ttaagtgaac
     2341 caataggtag gagaacatta tcaaatgtat tagagattag tgaaagaata atcaggtcag
     2401 aaactgagtt tttaaaagaa caaggactta tagacgtagc tgtatccggg atgactatca
     2461 ctaaagaagg ttctgatttg ttagataaac ttaaggacat catgagtgac atcatgggat
     2521 tgtctaagtt acaagataga gtcagagaaa agcttggaat aaaaagggta atattagttc
     2581 ctggaagctg tgatgaaaat ggaagtttac taaaagatgt aactagatat ggatgcgagt
     2641 atttcttagg tattttaaaa gatggagata tcgtttcaat aacaggagga agcactatgt
     2701 tggagttttc aaacgttatt aaaactgata agagataccc aaactctaca atagttccag
     2761 ctagaggaag tatgggaact gatgtagaga tgcaatctaa taatatagtt gcttcgataa
     2821 gtaagaaatt acattctaat tgcaaacttt tacatatacc agatgagttg gatgaaagtg
     2881 caatgaaaac tcttagccaa gtgccagaga ttaagacaac tttggagtat attaaaagaa
     2941 cagacatttt agtttttagt ataggtagag cagatgtcat ggcaaagaga agaaaacttc
     3001 cagaagacaa ggttgatgag atattatcta aaaatgcagt tggagaagca tttggatatt
     3061 atttcaacaa agatggagaa atagtttaca aactgaacac tgttggaatt gatttagaaa
     3121 cagttaaaca agtaaaagaa actattgcaa tctttgcagg tactgaaaaa gtagaagcct
     3181 tattagctat atcaaatatg cataaaaaca tggttcttgt aacagatgaa gaaagtgctt
     3241 ataagatatt gtcattatta taaaagtcaa gacctagcac agagctagaa aacatacatt
     3301 tactttatag gaggtagcga aaatggttaa agtagctata aatggatttg gaagaatagg
     3361 aagattagca ttaagaaaaa tgatggagca acaagataag ttcgaagttg ttgcaataaa
     3421 cgacttaaca gacgctaaaa tgttagctca tttatttaaa tatgatacag ctcaaggaag
     3481 atttaacgga gaaatagaag ttaaagaagg tgcttttgta gtaaacggaa aagaaataaa
     3541 agtaacagca gaaagaaatc ctgctgacct accatgggct gaattaggag tagatatagt
     3601 attagaatgt acaggattct ttacatctaa agataaagct gaagctcata tacaagcagg
     3661 ggctaagaaa gttgttatat ctgcaccagc tactggagat ttaaaaacaa tagtattcaa
     3721 cacaaacagt gatatattag atggttctga aacagttata tcaggagctt cttgtactac
     3781 taactgttta gctccaatgg ctaaagtatt aaatgataaa tacggtatag aaaaaggatt
     3841 aatgactact atacatgcat acactaatga ccaaaatact ttagatggtc ctcatccaaa
     3901 aggtgattta agaagagcta gagctgctgc tggaaacata gttccaaaca ctacaggagc
     3961 tgcaaaagca ataggcttag taataccttc attaaaagga aaattagatg gggcagcaca
     4021 aagagtacca gttgtaactg gttcaataac tgaattagtt tgtacattag gaaaaaatgt
     4081 tacagttgaa gaaataaatg cagctatgaa agaagcttca aacgaatctt tcggatatac
     4141 tgaagaaatg ttagtatctt ctgacataat cggaataagc tatggttcat tatttgacgc
     4201 tactcaaaca aaagttatgg aagtagatgg aaaacaatta gttaaagttg tttcttggta
     4261 tgataatgaa atgtcttata cttctcaatt aataagaact ttaggatact ttgcacaatt
     4321 agcaaaatag tcagttttga gaaaatagaa aagtagattg acaaagtccg agtttgtagt
     4381 ttaatactac ggactcagga ctttttttaa gaaaaaatat ataaaataca cctaaataat
     4441 gttacatact atatataaaa atatgataat atgatatatt taatagggta aaagtatgta
     4501 ctatattata cttggcaata aaaaattact ttattaaaag gagagatatt tatgtcaatg
     4561 cttaacaaaa aaactataga agatattgat gtgtgcggaa aaaaagtatt agtaagatgt
     4621 gactttaatg tgccattaca agatggtgta attactgatg aaaatagatt gaacggagct
     4681 ttaccaacta ttcaatattt aatttctaaa ggtgcgaaag ttatattatg ctctcactta
     4741 ggaaagccaa aaggagaagc taagccagaa ttatctttag caccagtagc aaagagatta
     4801 tctgagatgt taggaaaaga agttgtattt gcagcagacg ataatgttgt aggagaaaat
     4861 gcaaagaaag caactgaaaa aatggaaaac ggagatgtag tattattaga aaatactaga
     4921 tatagaaaag aagaaactaa aaacgaagag aatttctcta aagaattagc atcacttgct
     4981 gaaatatttg taaatgatgc gtttggaact gctcatagag cacattgctc aacagttggt
     5041 gcaggtgagt tcttacaaga gagagtttgt ggatacttaa ttcaaaaaga attaaaattc
     5101 ttaggagaag ctgttgcaaa ccctgtaaga ccatttacag ctatacttgg aggagctaaa
     5161 gtttctgata aattagctgt tataaacgaa ttattagaaa aagttgataa tctaataata
     5221 ggtggaggaa tggcttatac attcttaaaa gctcagggtt atgaagtagg aacttcttta
     5281 cttgaaatag ataaagttga atatgctaaa gaaatgatgg aaaaagctaa gaataagggt
     5341 gttaacttat tattaccagt agacgttgtt atggcagacc actttgcacc agatgctact
     5401 ccaatagtaa cagaagatgc taatgtaaaa gaagattaca tgggactaga tatgggacct
     5461 aagactatag caaactttgt aaaaactata aaagaatcaa aaactgtagt atggaatggg
     5521 ccaatgggag tatttgaatt tgaaaacttt gcaaatggta cattatcagt agctagagct
     5581 atggcagagt tgactgatgc aactactgta attggcggtg gagatagtgc agcagcagtt
     5641 aatcaattag gatttggtga caagatgact cacgtatcaa caggtggagg agcatcacta
     5701 gaatttttag aaggtaaaga attaccaggt attgcagcat tagataataa ataaactaat
     5761 atatagagga gattgtaaaa tgagaaaacc tataattgca ggaaactgga aaatgcataa
     5821 aacaataaaa gaagctttag aatttgtaaa tgaagttaaa gataaagtaa attctgataa
     5881 agtagaagct gttatatgtg caccatttac tttattaaag gatttaaaag aagctactaa
     5941 gggtacaaat ataaagatag gtgctcaaaa tatgcacttt gaagaaaaag gagcatttac
     6001 aggagaagtt tcacctctta tgttaaaaga aatagatatg gactatgttg taataggaca
     6061 ttcagaaaga agacaatact tcaatgaaac tgatgaaact gtaaacaaga aagttttaaa
     6121 agcattagaa gtaggaatag acccaatatt atgtgttgga gaaactttag aacaaagaga
     6181 agctggaaaa actaaagatg tttgtaaagt tcaagttgaa aaagctttag aaaatgttct
     6241 taaagatgat ttagcaaaag tagttgttgc atatgagcca atctgggcta ttggaactgg
     6301 taaaactgca actgctgaag atgctaatga cgttatatct tatataagag aagttataaa
     6361 aggattatat ggagaattag ctaatgaagt tagaatacaa tacggtggaa gtgttaaacc
     6421 ttcaaatgta gccgaaataa tgggtcaaag tgatatagat ggagctttag taggtggagc
     6481 tagtttagct agtaatgatt acctagacct tgttaatttc taaggagtga aatgatgaaa
     6541 aaaccagttg ctttaattat tatggacggg tttggataca ataaggatgt taagggaaat
     6601 gcaattgctg agtccaaaac tcctaattta gatagaataa aaaaagagta tccaaacaca
     6661 ttgataaatg ctagtggtct tgatgttggt ctacctgatg gtcaaatggg aaattcagaa
     6721 gttggacaca ctaacatagg tgcaggtaga atagtatatc aagatttaac tagaataaca
     6781 aaatctataa aagatggtga cttttttact aataaagttt tatgtgaagc aatggataat
     6841 gcaaaagaaa attctcttca tgtaatggga cttttgtctg atggaggagt acattcacat
     6901 atagaccatc ttaaggctat aatcaaaatg gcaaaagata aaggcgtaca aaaggtgtat
     6961 gttcacgcat ttactgatgg tagagatact gaccctcaaa gtgcattaga atatgccaaa
     7021 gaagtacaag ctagtatgga tgaaattgga gtaggtgaat ttgctacagt ttctggtaga
     7081 tactacgcta tggatagaga taagagatgg gaaagagtag aacttgcata caatgccatg
     7141 gttagaggta ttggtgaaaa agcaaattct attgaggaag ctattcaaaa ctcttatgat
     7201 gatggaaaaa atgatgagtt tataatgcca acagttatta tgaaagatga taaaccagtt
     7261 ggaagtataa aggaaaatga ttcaattata ttcttcaact ttagaccaga tagagctaga
     7321 caaataacta gagctttagt ttgtgaagag tttgatggat ttaaaagaga agatattaaa
     7381 aacttctttg tatgcttaac agagtacgat ataactatag agaatgtgca tatagcattt
     7441 ggacctcaat ctttagccaa cactctaggt gagtatctag ctaaaaatgg aaaaactcaa
     7501 cttagagctg ctgaaactga aaagtatgca catgtaactt tcttcttcaa tggtggagta
     7561 gaagagccaa ataaaggtga agaaagacta ttgatacctt ctccaaaggt tgcaacttat
     7621 gatttaaaac cagaaatgtc agcatatgaa ttgacagata aagctttaga taaattagga
     7681 gaagataagt ttgactttat agtattaaac tttgcaaatc ctgatatggt aggtcataca
     7741 ggaagtatag aagcggctat aaaggctgtt gaaactgtag atacttgtgt aggaagattg
     7801 atagataaaa tagtagagtt aggtggaagt gcaataataa ctgctgacca cggaaatgct
     7861 gaatatatgt tagacccaga aacaggaaaa actgtaacgg ctcactctat taatccagta
     7921 ccatttattg tagttggtca agaatatgaa agtgcaaaat tattggatgg aggaagatta
     7981 tctgatatag caccgactgt tttagatatg atgaagttag aaaagccaga agaaatgact
     8041 ggtcattcac ttatttcaaa atagtcgatt ctgataagta gagaataata tataaactac
     8101 aatataaatt taaaaataat gcagtttagg tttattttgt aaagtttaga ctttatgaca
     8161 gaagatttag attttgtaag ttttggatat tgtaaggtgt agatgaagat atattttata
     8221 aaatttacaa gatagacaat ttataaaata aaaaattata atatagataa taatgtaaat
     8281 aa
//
LOCUS       DBGJFU010000153         8204 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.156, whole genome shotgun sequence.
ACCESSION   DBGJFU010000153 DBGJFU010000000
VERSION     DBGJFU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.156"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            94..849
                     /locus_tag="ACOIAS_003301"
     CDS             94..849
                     /locus_tag="ACOIAS_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2702988.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            980..2002
                     /locus_tag="ACOIAS_003302"
     CDS             980..2002
                     /locus_tag="ACOIAS_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD0873 family tyrosine ABC transporter
                     substrate-binding lipoprotein"
                     /protein_id="HGZ2702989.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2166..2723)
                     /locus_tag="ACOIAS_003303"
     CDS             complement(2166..2723)
                     /locus_tag="ACOIAS_003303"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HGZ2702990.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2729..3805)
                     /locus_tag="ACOIAS_003304"
     CDS             complement(2729..3805)
                     /locus_tag="ACOIAS_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HGZ2702991.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3806..4489)
                     /gene="modB"
                     /locus_tag="ACOIAS_003305"
     CDS             complement(3806..4489)
                     /gene="modB"
                     /locus_tag="ACOIAS_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGZ2702992.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4638..5450)
                     /gene="modA"
                     /locus_tag="ACOIAS_003306"
     CDS             complement(4638..5450)
                     /gene="modA"
                     /locus_tag="ACOIAS_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2702993.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMEDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5643..6572
                     /locus_tag="ACOIAS_003307"
     CDS             5643..6572
                     /locus_tag="ACOIAS_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGZ2702994.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6733..7182)
                     /locus_tag="ACOIAS_003308"
     CDS             complement(6733..7182)
                     /locus_tag="ACOIAS_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702995.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7385..>8204)
                     /locus_tag="ACOIAS_003309"
     CDS             complement(7385..>8204)
                     /locus_tag="ACOIAS_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2702996.1"
                     /translation="KIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTR
                     DTMILVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDS
                     LVKFVKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMG
                     VLEKRIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCN
                     YQNSKKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2845 a         1356 c         1004 g         2999 t
ORIGIN      
        1 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
       61 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      121 agcttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      181 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      241 aatataatat ccgggcttat aaaagaatct tctggtcaag tattattaaa taaacataac
      301 ttgtctaatt tagctgaaca cagaagaact caaatagtaa gtagagtttt tcaagaccca
      361 agtcttggaa cttgcccttc tatgactgtt agagaaaact tgtctttagc tcttaacaaa
      421 ggaaaactat taaatctaag aaaatgtctt cgttacaaaa gagatttctt agaaaattta
      481 ttggaaggtg tatctcttga tttaaaaaaa taccttgatg ttcaagtaaa atttttatca
      541 ggaggtcaaa gacaatcatt atcactaatt atgtcgtgct taacaaatcc atcagtttta
      601 ttacttgatg aacatacagc agcacttgac ccaaaaacat caaatgaagt aattgaattg
      661 actaataaga ttgtaaggga aaaaaatata actacactta tggttactca caacttaaaa
      721 cacgctcttc attatggtga tagattgata atgcttcata agggtgaagt tgtattagat
      781 gtaaaaggta aagaaaaaga acaattaaca gttgaagaaa tcttagaaaa atttgaatat
      841 gcagtttaaa ataatatatc tattgtatta aactcggctt taaaataaag ttaaaattac
      901 aataattatg gttactttgt aataattcat atgaatatat gaacatatat aataataaat
      961 ttttgagggg gatataaaaa tgattaataa aaaaagattg gcaagtttga ttttagctgg
     1021 tgctcttagt atatctatgc ttacagggtg ttcacaagga ggagactctg gtaattcaaa
     1081 acaagaaagt aattctaaag gtaaagaagt taaaaaaatt ggtataactc aactggttga
     1141 acatccagca ttagatgcca ctaggacagg ttttgtaaaa gcattagaaa agaacggatt
     1201 taaagatgga gaaaatattg atatagactt ccaaaatgct caaaatgaca tgcctactac
     1261 acaaagtatt gcgagtaaat ttgcatctga taagaaggac ttaatatttg ctatatctac
     1321 tccatcagct caggcagcat ttaatgctac taaagatata cctatactta ttactgctgt
     1381 gagtgaccct gttgctgctg gattagttaa aactcttgaa aagccaggca caaatgtatc
     1441 tggaacatca gattttgttt ctgtagacaa aggtttagaa cttcttaaaa tatttgctcc
     1501 taaggcaaaa actataggag ttatgtacaa tactagtgaa gtaaattcca aagtccaagt
     1561 tgatgcttta aaagaatatg catctaaaaa tggatttaaa gttgttgaaa aaggtataac
     1621 tacttcaaat gaagttaacc aaggtatttc tagtttagtt ggcaaaatag acgtattata
     1681 tgttcctaca gataacttag tggcttcttc tatgcctata gtctctaaaa tagctactga
     1741 gaataaaata cctgttatag cagctgaatc tgggccagtt gaaaaaggtg ctcttgcttg
     1801 tcaaggtatc aactacgaaa aactaggtta caaaacaggt gaaatggcag ttaaaatttt
     1861 aaatggtgaa tcagtatctg atatgcctgt ggctacatca gatgatacag atatcattgt
     1921 taatgaagat atattaaaag ctttaggtat ggaaaaacct tctaatgaaa atatttctta
     1981 tgtaaagact aaacaagaat agaaagatat tttctatata cgattaatat gcctgtttct
     2041 ataaattaat ttatagaaac aggcatatat ttttatttta atgtattatc tatttaaaga
     2101 ttatatgtaa atgctcttaa tttgtttgta taaattttac ttgtataaac tttatacaat
     2161 catatttatt cttctatttt ctttattact ctacatggat ttccaacagc tacagtattt
     2221 ggtggtatat cttttgtgac tacacttcct gcacctatta ctacattatc acctatagta
     2281 attcctggtg ttatgattac tcccccacct atccatacat tatcacctat ttttacagga
     2341 gaaccatact ctatacctga atttctaagt tgtgcatcaa ttggatgata ggctgtatag
     2401 atttgaacgt ttggagctaa cattacattg tcacctattt caattttaca tacatctaaa
     2461 aaaatacaat cataatttgc aaagaaattt tcgcctacat gtatgttata tccataatca
     2521 cacctaatat tttgttctac atttatttgc ttgccaactg aaccaaatag ttgtcttaaa
     2581 atatcttctc ttttttcata ttcatcctct aaagtattat taaacaatct agttaatttt
     2641 ttacaatact ctctttcttt tactaacaat tcatcatttg catagtatcc tttcccagac
     2701 aacatttttt ctttttcagt catatagttt actccttttt gttataattg tatctaaagc
     2761 aaaataaata ttcctgtgga aaatctaaat atattctgtc acctttctta aatctcattt
     2821 tacttttttc caattctatt tgaataggta cacattcgtt agataaatca gtttttctaa
     2881 catatatagt atatgtaaaa ggattttcta caatgttaat caattcgaaa cataactttt
     2941 catttaaatc ttcattagaa tccaagacct taatatgatg ctccctgatt cccacatatt
     3001 ctatattatc acctatttct ctatttaatg tcaactcgca tccccagtct gtagcataaa
     3061 tagtattttt atccagtcta tttaatttgg atatattttt acaccctgtt atcttagctt
     3121 ctataaggta ctttggagat tcaaagagct cttttttagg tctctttgga agaccttctc
     3181 ctttgttgta cactataata tcatcacaaa ctcggtaagc ttcttcaata tcatgagtaa
     3241 caaatagtat atctcccttg taatctttta acatattcat aagttctttt tccatattgc
     3301 ttcttaaatg atggtccaat gctgagaaag gttcatctaa aagtaatata tctggagatg
     3361 ttgccaatgc tctagctagt gctacccttt gttgttgacc accagataat tgccaagggt
     3421 atctcccctc aaacccattt aatttaagta tatctaagta ccttgctact atttcctttt
     3481 tttcactctt acttagtttt aaaagcccaa gttctatatt ttgacttaca gtcatatgtg
     3541 gaaacaaagc atagttttga aataaaaatc ctacctttct atcttgtgtt gataaattta
     3601 tcttcttttc agaatcaaat agaactctat catttaatac aattttacct cttgttggtt
     3661 tctcaagccc tgctatacat tttaatgtca tactttttcc agaaccagat tctcccaaaa
     3721 accccagagt acctttttct tgtttaattt ctacctttag tttaaaagag gataaatctt
     3781 tttcaatatc cacgtataag ctcaactatc cacacctctt tcctataatt ttctgctgaa
     3841 attccgacca ataatttagt aataaaatca ttgctataga tattgctaca ataatcataa
     3901 cccacatcat agctttattc atatctccgc cttcaacagc aaaaaatata gctattggca
     3961 tagtttgagt ttttccaggt atatttccag ctatcataag tgtagcacca aattctccaa
     4021 gagctctggc aaacgacagc accaatccac caattattcc aggccatgct aatggaactg
     4081 caatcttaaa aaatactttc cattcattaa gacctaatgt tctagcagct gacaacatat
     4141 tattatcaat ctgttcaaaa gctgaccttg aagttctata catcatagga aaagacacca
     4201 ctactgctga aattacagtt gctgtccaac taaatattaa agtcctatcg aaatttaata
     4261 aaaactttcc tataaatcca ttcttcccac atagtaataa taaaaagaaa cctacaactg
     4321 ttggtggcaa aattagtgga agtgtaaata gtccatctat aagacccttc cacttacctc
     4381 tatagttagc cataatatat gacacaaata ttcctattac aaaagttatt attgttgaca
     4441 aaatagatgt cttaagtgat atccataagg gtgaccaatc tgttcccatc tttccctcct
     4501 taattttaaa tggaaatagt gaaactaata actctattaa tttcactatt tccattttat
     4561 tataatacac aattattttc aattttcatt tatttatata taatttatac ttaattacac
     4621 ataatttatt tctaagttta ttctactttt ttatatccaa attcttcaaa aatcttttgt
     4681 cctgactcac ttaataaaaa ttcttcaaat ttcttagcag catctacgtt tttactagct
     4741 ttaattactg aaactggata tgtaataggc gaatgagttt tttcgtctgt tttttcaact
     4801 acttttatct tatcattatt gacagtatca ctgaagtaaa cgaatccaac atcagcattt
     4861 cctgattgca cccatgctaa aacttctttt acatcttttg caaatactag cttatctttt
     4921 aacttatctt ttaaatttaa attagttaac acctcatctg catacttacc agctggaaca
     4981 ctttcagctt caccaacagc aattttcttc actttatctg tagtcaaatc ttccatacct
     5041 gatacactac tgtcagcaga agaaataaga actaaatcat tttttactaa atctttcata
     5101 gtatcagaaa ctaataactt ctcttcatcc aaagctttca tttgtttttg acctgctgaa
     5161 ataaataaat cacaaggtgc tccttgttct atttgttgtt gtaatgagcc tgatgctcca
     5221 tagttaactg taagttttac atttgaatca acttttttat attcttcttc aatctttgcc
     5281 attgcttctt ttaaacttgc agcagccgat atatttaact ctactgaatc actactttta
     5341 gttgtttttt cttgttttcc atcatctttt ttgtcttcat tactattgca cccaactgtc
     5401 cccaaagtta aacatgcaac taatcctaat attcccaata tttttttcat aacaatctcc
     5461 ctttttgtaa atttgataca cattttaaca tgttttatta tgttttatca tgttttgtta
     5521 tatatagttt aattatatat acatgataat aaattttcaa gtaatttatt tacactactt
     5581 ttaaatattt tgctacattt tccttttttt aatgttaaaa taaccttata tggaggtgga
     5641 ttatggactc attatcatct ttgacacctg tcgaggtagc tgaattatta aagataacaa
     5701 aaaatactgt atatgaatta atcaaaagag gtgaacttcc ttcttataaa gtagggaaaa
     5761 aaataagagt tgatactaaa gatgttgaag aatatatcaa cagccaaaaa actggtaaag
     5821 taaaggatac acatttcaac aataaaaata tagatataaa taataatttc aaaattatta
     5881 tttctggtca agatgtaata ttagacattt tagctaggtc tgttgaaaaa aaattggatg
     5941 gaataagtac atttcgctct tatataggta gttataatgc tttgtatgag ttatataatg
     6001 gcagagtttc tatagcatca tcccatctat gggatttcga aacggatgaa tataatagca
     6061 cttttgttaa aaagctattg ccaggaatac catgtgtact tataaatcta gcttatagaa
     6121 tgcaaggttt ctatgttgct aaaggaaatc ctaaagagat aacaacctgg gaggatttaa
     6181 caaaaccaag tataaccatt gtaaataggg aaaaaggttc tggaactagg gttttactag
     6241 atggtaaact tagacttcta aactttaatg gtaaacatat aaaaggttat gataatgaag
     6301 aattatcaca tcttggagtt gcaagtactg tttctcgtgg aatagcagat gttggtttgg
     6361 gaaatgaaaa agctgcttta caagtaaata atatcgactt tattcctctt cacaaagagc
     6421 gctatgattt agttattaaa aaagaagact tacaaaatcc agtttaccaa actataatta
     6481 acataataaa ctctcctgag tttaaagctg aacttcaagg tcttggcgga tatgatttaa
     6541 ctgatactgg aaaaactata gcaaaagttt aagattaaaa tcgcaatcta ttttataaga
     6601 tttataattt ttcacaaaat ttataactta aacacgaaat aaattgcgat tttagtgtct
     6661 caaaaaattt atgtctttca ttttatcatc caatacaaaa tataatataa ttaattgtgc
     6721 aatcatatta tattaatata tatttacata atagtatttt tctttaatgt agattaacat
     6781 atgatatgtt ttaaacatca atccccttgc tttcttatat atatttggca acacatatga
     6841 gtttaataaa ttatatacaa tgttgctttc ttcttgtatt ttttcaacca acaagttatt
     6901 tataaaatta cctttctcat tttcaataat ctcactccaa tttatcttaa cttttaatat
     6961 atatcttccc actaggtata cacactctac aataaataat aaaaatacaa tgcagtcagt
     7021 acctgttgtc ttatataaat tacccgatac taatggcttt gcgaagaaat tataaaaatt
     7081 caaagtatca cccgtataat caaaaagtat taatagcact cctagaactc tcaatacttt
     7141 aatattcaac atgtttttat ctttattatt tttgtttttc atatatacta cctctctttt
     7201 ctttagattt gattgtaatc taatactaac tcttttattt gaggtataaa tgaatttaat
     7261 tggtagttat tttcattttt actagaaatt aattcacatt actaaacaaa acttatattt
     7321 aaacataaaa atagcctatc tatcttttaa taaatacaaa aataaataag ctactaatat
     7381 tcaattaact tttatcactt aaaacattac tatctataaa tttaaatatt ttattgaagt
     7441 atactgtact atcatgttca aatccatcaa catgaccaat accttctata agccataggt
     7501 cttttttatc atgtttaact aaatcataca ttttcttgct attttgataa ttacaaacag
     7561 tatcttttgt tccatgtata aacagtatgg gtacattaga tttctcaact gactcatatg
     7621 gttttacatc actgtaccaa aatccagatt taaacttatt atataaatct ccacaagtca
     7681 ccacataatt gattaatata tctggtattc gtttttctag tactcccatt ctaattgcat
     7741 ctttcatttc agaataagga gaatccagta tataaaaatc gaccttatca tcttttgaat
     7801 ttatctctga atgcatagca gctgtccctg cccccatgga aaatccatgc acacctaatc
     7861 tcccttcagg aaatttattt tttacaaatt taactagact atctaaatca tacctttcat
     7921 aaagaccaaa tgtgtaatta tctccaccac tattaccagt atttctttga ttataaacta
     7981 atacattata acctttatct aaatatctat atgctacctt taacatttca tagtaactac
     8041 ttccaattcc atgaacaagt atcatagtat ctcttgtctt tatatttgaa gttataaaaa
     8101 gactttcaag cttgtaatta ttagaacttt gtatcattaa ttcagatgtc ttgtagtttt
     8161 ttaatttctc cagtggttta tcttttctct cagaaaatat tttt
//
LOCUS       DBGJFU010000154         8049 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.157, whole genome shotgun sequence.
ACCESSION   DBGJFU010000154 DBGJFU010000000
VERSION     DBGJFU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.157"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(39..260)
                     /locus_tag="ACOIAS_003310"
     CDS             complement(39..260)
                     /locus_tag="ACOIAS_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702997.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(762..1742)
                     /locus_tag="ACOIAS_003311"
     CDS             complement(762..1742)
                     /locus_tag="ACOIAS_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2702998.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1906..2778)
                     /locus_tag="ACOIAS_003312"
     CDS             complement(1906..2778)
                     /locus_tag="ACOIAS_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGZ2702999.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(2942..3493)
                     /locus_tag="ACOIAS_003313"
     CDS             complement(2942..3493)
                     /locus_tag="ACOIAS_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2703000.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(3513..4415)
                     /locus_tag="ACOIAS_003314"
     CDS             complement(3513..4415)
                     /locus_tag="ACOIAS_003314"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGZ2703001.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDD
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4430..6802)
                     /locus_tag="ACOIAS_003315"
     CDS             complement(4430..6802)
                     /locus_tag="ACOIAS_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HGZ2703002.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(7023..7712)
                     /locus_tag="ACOIAS_003316"
     CDS             complement(7023..7712)
                     /locus_tag="ACOIAS_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703003.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVYDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
BASE COUNT         2584 a         1455 c          762 g         3248 t
ORIGIN      
        1 tataaatgat atatgaaatt acatatgttt atattatatt aaattttatt attacatttt
       61 ccacaacaat actcatatgt ctccacatta tttggaatat ttacaaatac aaattcttta
      121 cagttctcac aatactttaa tatagatata gttccacaag tgtaacattc aaatattaca
      181 caatcctcat tttcctctac tatttcaaac tttttactat gttcaccaca ctctgggcac
      241 ttgtgattat ttacattcat aaatactcct ccttattagt atagttacat gtcaaaaact
      301 taattaatgt attctatatt acttattata tattctataa taaatcatat tctaatataa
      361 taaaacaaat taaataaaaa aaaaaccata aattttaaat ttatggatta attcttgtaa
      421 tgataactct atttgaggat gtgagggagg gttatcatta caatagggta ttatattatg
      481 gtttttaagc tagtctttct acacctcata tgatacatat gctatatgaa ttttatatga
      541 tttttacata aatattttat gaaatttaaa ttttttagtc attttgaaat ttgtaaaaag
      601 ttctctaaaa atccacagca tcataaacag ccaaaattta agtaaaacat cataaaaaaa
      661 tttgtccaat aattttaaca agtaatctca cctcattata attattgaca caaacttaaa
      721 taaacaatct ataaatcttc tataaatctt aaactttccc cctattgata cacaatttct
      781 agtttgtcaa ttttattttg catttctaag aaaatttctt caacagaatt gttaaatata
      841 ttgcctaaat agattctttt atctttttga aatgaataag attttacact accatcagaa
      901 gaaatatata aatgactcca ctctgctcca tattttgaaa acctctcctt tgtatcttta
      961 aacctcatat cttctatttt tattgctttt tctctgtctc ctcttactac agtgtgaccc
     1021 ctttttatta aactggttat ttttatatct tcttttaatt taatacgtgt atcatcattt
     1081 tttgaatata ctttccatat cactttatga cgataattat ctaattcctt tattaagctt
     1141 ctatcaatag taaaactatc tttagttacc acaattaaat caaaatggtc taacatatat
     1201 tgaaatattt ctctcatact tttatttttt aaaatatcct caccaataac ctctataaat
     1261 ctacagcctt tctcatacat gcagttcaat aaacttgtga tttcagaaaa ataatcattt
     1321 gtattattat ctccatctat ttctatagta aagtacaaag gagtaatgta tgatttattt
     1381 cctgttatct caaatacact tgatagtgtc tgtatatcat cttcaaacgt taagtacaca
     1441 ttctcataaa acataaaaaa ttccataact ttttctttca tattatggat atttctatat
     1501 ctattggata atatatttat tatatcgtca gtagtatttt tcccatcaca ttcagccagt
     1561 ataacaattt gcttccaact taagctatcc acatttccga tacttttttt atatccatct
     1621 tcttcgtcat aaataggacc aattataatg ttttcttttg gccacagatg aaccctagtt
     1681 ccatctttta atttcggata tttatttttt acagactcat aaaatctact tgtatcaccc
     1741 atgattcttt actacccccc atagtttaaa tattctatgc taaaaatagc actttatttt
     1801 tattaattca acaattattc aaaattggtc attatatatg tttaaatgga tttaacatca
     1861 accttattta tatgttattg atgtttatat atgttatata aaatcttatt tctcatactt
     1921 aaatgtaatc ttaactatct ctggtttaca attaagccta attaaaactt ttgagcctcc
     1981 taagccttta tttaaaatca tagtgctatt atctttttca tattttcccc agtcatactt
     2041 aggaaagaac tctctatttg gagataatac acctccaaca aaaggtattc ttattatacc
     2101 tccatgaaca tgtcctgcta aaaccaaatc tgctccccat tttgcatatt tttcaaagaa
     2161 aaatggtgta tgtgctaaaa gaatgttata ttcattttca ttaactttac ctagattgtc
     2221 ttctacaaaa ttgtcactta gttctaattt tttgttatta gcaaataaat atggatagca
     2281 ttccaatggt ataattaaac catataaatt aagatggcta ttacctctat atattcttac
     2341 agattcattt tccatattta ttattttctt tgaatatagt tccttaaaat aatccttata
     2401 tagatgttta tactttttta ttaaagattt ttgttcatga tttccaatta tatgatatac
     2461 ttcatatttt tcaaccaaat tatctattaa atctaatgca acttgaaaat ttttattttc
     2521 tccatctact aaatctcctg ttataaatat aacatctggg tttaatgatt caattttatc
     2581 tatcaatacc ttattatttt tatcaaacac cttattatgt aaatcactca cttgaactat
     2641 tttaaagtta tcaaattcct ttggaatttt tttgttgaaa atagtatatt ctgtcgtttc
     2701 tatatgctga gtttcatact tatacagaat atacaataat attaatacta atactgtcat
     2761 tgaaaccttt ataatcatat tctcttcctc tttattctat tttgttaata ttattataat
     2821 gcttttatgt tttttttaaa acaataacat catcacatac atataaatct tttcgacatt
     2881 ataaaaataa gttatccttt tctaaaattt tcatttattt tagaaaagga taactataaa
     2941 attatctata aacctctata ttgtgaaata tagtttttcc tttatttaaa tttttatata
     3001 tatgctctaa agttccatca aacctaatag aatgtccaga ctctaaaata taagttttcc
     3061 catccacatt aaggtcaagt ttaccttcta ttacgagcac atattcttct aaaacatgac
     3121 tatgtgtaga tgaaacatga gtacagtttt cctcaagctc aattataaaa agttcaaacc
     3181 ttctttgagg gctaaaaggg tatattggat ataacttcat gttccctttt tgctcaacaa
     3241 taggctctac ttcttctttc tttataacct cataggtatt tgtattttca tttaatagtg
     3301 tagtaaaatt tattttaaat ccagatgaaa ttttccataa agtagatact gtcggacttg
     3361 attcacctct ctcaatctgt ccaagcattg ctttactgac acctgtcatc tttgaagctt
     3421 tgtctaaact tatgcccatt tcttgtctca ataaccttaa attttctccc acattaggaa
     3481 atttattatc catgttaaaa tcctcctgat agctatttgt attctatgat ttttactcct
     3541 gtaagttcta ctatctcttt taattttaac atatgattgt tacaaatatc atcaaattca
     3601 tatatataat cttcaagccc taatgcctta tatttatcga ttccaaattt atgatatgga
     3661 agtaactcca tcttgcctcc tggaacattt tgtttgacaa ataatgcagt atttcttata
     3721 ttttcttcac tatcatttac tccataaata attggcactc ttataaccat agatttattc
     3781 aactttgaaa gcctacatat attatctaat ataattttat tacttactcc tgtatattct
     3841 ttatgaatat tatcatccat agattttata tctacaaata tgtggtctat tttctcaaat
     3901 acatcattta ccttattcca atcaaaatat ccacaagttt caattgcaat atttatgcct
     3961 ttatcataaa aaatatcaac taattctctt aaaaaatcaa tctgaagtgt aggttcacct
     4021 ccagaaaaag ttatccctcc attagattca aagaaaaata taaaatcttt ttcaacttcc
     4081 tctactattt cttcaacact catttcttcg gtcattatac aaatggcatc tttaagacat
     4141 accttgatac actctccaca taaatcacat ttattattta tttgcgctct atcaaatagt
     4201 gaacttatat tttgaggaca aacatctata catctattac aggatacaca tttatctttc
     4261 ataacaccta attttactat gttactccag gaatctggat tagaacacca cttacatttt
     4321 aatttacatc cttctaaaaa tatagtagtc cttattccat ctccatcatt tactgaaaaa
     4381 tgttgtactt ttacaactct acctttttta ctcataatct acctcataat taaaaattgc
     4441 tatgtgaatt tctagctatt atagcatctt gcatttctct tgataaatta acaaattgag
     4501 tactatatcc agcaaccctc actaataaat ctttatattc ttctggattt tcttgagctt
     4561 ttaatagagt atcatttgat atcgtattaa attgtacatg gaatcctcct aaagcaaaat
     4621 atgatttaat catagatgtt agatttctaa gtcctctttc agtttccacc aaatcttgat
     4681 tcagtcttaa atttagaagt gttccaccag tatgaacatc atgatttatt tttgatgcag
     4741 atttcattgc tgctagtgga cttgttgtat cagtaccaac aaatggagaa actccctctg
     4801 tcaaaggttt tgtcgctttt cttccacaag gagttgcacc tactattttt ccagttggta
     4861 catagtttga tatacccata aatgcactat taaatttaga gccaaaaata tccttgtatt
     4921 ttctagtttc agtataataa aaatcagaaa cttcaattgc taatgaatct acataatcat
     4981 tatcatttcc atacttagga acatccaacg caagttttct taattcttca taaccttccc
     5041 aatcattatt tagtgctttt gttaattctt ctaacgtagt caccttatcc tcaaatacta
     5101 atttttttat agctgccaat gaatttgaaa ctacacctaa ccctatacca gttaatacag
     5161 gacctatatt atattttgct cctcctttac ttaaatcaac tcccttatcc aaacatccat
     5221 ctacacaagt agataaaaat ggtcttggta ccatttcttt atgaatttgt tgagcaacag
     5281 tagttcctat aacagaactt tttacaagat atgctaattg ctttaaaaac tcatccttaa
     5341 gttcttcaaa agaagtaaat tctgtgatat tttttgtatc taaccccatt ttttctccag
     5401 ttaatctact cttaccttca ttcatagcat attctaaagc tgctgcaaaa ttgatattta
     5461 cggctgaagt ccactgtcct gtttttctaa aatgaggaac tacacaacca cagttactcc
     5521 aatctctagc atcttctgca tcatatccat cttgtaataa catttgtgcc cctgcactat
     5581 cgctgtgtat agcagggaaa ccagttcctt gttttaccaa tttagcaact gccattacga
     5641 aattatctgg tgctccttga ctcactctaa cacttaatcc tggttggtga gtttttacac
     5701 tttctgtagc ctttaaacac atatatgaga tatcattcgt tccatcagta ccatttctct
     5761 ttttaccacc aacagtaaga ttttggaatt gattatatcc tgcaaagtaa tttgctgtat
     5821 ttgctgatat agtccatacc cattctgata acttaatcca aagtgcttct atcaattctt
     5881 gagcttctga ttcagttatt ttaccttctc tagcatcatt ttcataatat ggatacatat
     5941 attggtcaaa tctacctaaa tttaatgcca atggattttc tgatagaata ccacctaatt
     6001 gaacaaacca tacaaattgt attgcttcat aaaaattagt tggagggttt gctggtacat
     6061 tcatacatat ctctgaaatt tttataagtt ctaactttct cttttcatct gtttcttgct
     6121 tactcatctc ttttgcaaga tttgaatatc tttgtgccaa tgtcataata ccctgtgcaa
     6181 ctatagaaac agatttgtaa aaatctatct tttcaatatt ttctgaaact gatatatcta
     6241 attctttaat ttttttatct gcatcttcct taatcccttt atatcctttt acaaacaata
     6301 tatcttgata atctggtgtt acttcaccaa ctgcctgtct ccacttagaa tcattatcta
     6361 ttattcctgt atctactaat atcttagctg tatcttctgg gattcttgct aagaaaactt
     6421 cttccaaaga ttttcccttc caatatggaa atatttcttt tcttataaat tctatttgtt
     6481 cttttgatat tacatatggg tcttgagttc ttttatcaaa agtatccatt tctttgtcta
     6541 cccattgcca agaaaactct ggtgtcaata tccctgttct tctaaatttt cctgctgtac
     6601 ctactattaa ttcattttca aagattttta taggaagttc tttacatgtc tccaaaaaag
     6661 ctttagctct tcttatacag atagcttctc cctcagtttt tttatgtgat tctgtaaaaa
     6721 ttctagccct ttcacaacat atcataggtt tagaattaat atattcttgt ctaaatgctt
     6781 ctattctatt agtttgactc atttaaaatc cccctttgga taatatatac tacatttttg
     6841 tatgttataa catactttaa ttatattata ctgtccttta gcataaaatg gaagcaaaat
     6901 aacttatttt ttttcatcta ccttgaacac ctatatatta atactttttt tgttaaattt
     6961 ataaactcta aaaaaatttt ctatgatata ttttctatat tgttatagtt atttctaata
     7021 tactaatgat ttgaaatatc aaatttgtat actagactta gttttaaaac cttagttgat
     7081 tgtttaactt tgttaacttc aacatgtata tccttcatat aaggaagctc tacaacacct
     7141 actcccttat taaagtcttc ttcaaccata gaaaaaattt ctttctcagt taagttcgta
     7201 ttagtcaatg acttaaataa attgtatata aatttaatat atttatcact ctcagataac
     7261 ttatcatctt ctggatactc tttagcacag agaatatcta ttgttacttt tccttcttta
     7321 tcgtcttcag acatagaata gaataaactt cgaatatttt tttcaggtgg tttttcagca
     7381 ccatttatag atactacaaa gctaccatct gattttgagt cagtaatact ataaccactg
     7441 tctattatag ctttcgaaaa tctttgagat aaatcttttt ttacttcttc tctacctttc
     7501 gcatcattaa tcttttgtga gtaattatta aaaagctcga tagattcatc ttttttatct
     7561 tcaacttgtt ctttcaacga atctttacta atttgttctg agctacttaa gttcttatca
     7621 tatacaggag acatacaccc taccatcaat gctaagctaa gatttaaagc cacaatgatt
     7681 gccactctat ttatattcct ttttactttc atacacaatc ctcccatcac actaaccgct
     7741 ctaaagtatt acttattgca ctagaatatg tttatatttt ctttatatta aagatttatt
     7801 attcaataca tatcaccttc ctatccaatt cttataactt actattagta aaattataag
     7861 ctccttaata cagaaataag gatgttatct tttgttatta aaagataaca tccttatttt
     7921 tttgtatcaa tacatatgtc ccaactggta acttaatcgc cccaactgtc agtatcttgg
     7981 tccaatggta tatatatatt tagtataaat ttatcgatac tattttcaaa taataattct
     8041 ccattatat
//
LOCUS       DBGJFU010000155         7903 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.158, whole genome shotgun sequence.
ACCESSION   DBGJFU010000155 DBGJFU010000000
VERSION     DBGJFU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.158"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            406..1707
                     /locus_tag="ACOIAS_003317"
     CDS             406..1707
                     /locus_tag="ACOIAS_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGZ2703004.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1783..2058
                     /locus_tag="ACOIAS_003318"
     CDS             1783..2058
                     /locus_tag="ACOIAS_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGZ2703005.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3501..3731
                     /locus_tag="ACOIAS_003319"
     CDS             3501..3731
                     /locus_tag="ACOIAS_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2703006.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3724..4305
                     /locus_tag="ACOIAS_003320"
     CDS             3724..4305
                     /locus_tag="ACOIAS_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGZ2703007.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSI"
     gene            complement(4880..5557)
                     /locus_tag="ACOIAS_003321"
     CDS             complement(4880..5557)
                     /locus_tag="ACOIAS_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HGZ2703008.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5746..7110)
                     /locus_tag="ACOIAS_003322"
     CDS             complement(5746..7110)
                     /locus_tag="ACOIAS_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGZ2703009.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7238..7759
                     /locus_tag="ACOIAS_003323"
     CDS             7238..7759
                     /locus_tag="ACOIAS_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGZ2703010.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3090 a          882 c         1363 g         2568 t
ORIGIN      
        1 aagatattac tatcaagtag tgaattatat tgaacgatat attaataatt ggaagtaagt
       61 tatatcaaat aagatattac tatcaagtag tgaattatat tgaacgatat attaataatt
      121 ggcagtaagt tatatcaaat aagatattac tatcaagtag tgaattatat tgaacaatat
      181 attaataatt ggtagtaaag tatatcaaat aagatattgc tattatgtag taaattatat
      241 aaaacaatgt atcagaataa atagtatatt aataatttgt agtatatcat gacgaataat
      301 acagtcataa caaatagtga gtcataacaa ataatgtatt tataattgat aatgtattta
      361 aaaatcaaaa tataaaaact tataaggaaa gagagattaa tttatatggg taaatcaaaa
      421 gatgttgaaa tagagaagtc tattttaggc acaattcttt tagacaataa attaagctat
      481 aagctagatg aattaaatga aaacatgttt atgaatgata tatgtcttga gatatttaaa
      541 attatgaaag aacttaaaaa agaaaacata gtgattgatg tggccactgt gaaatcaaag
      601 atagatagaa aatctctagc aataaaaacg agtgatgtga ctaatctaat tacttggggt
      661 cagaattttg gtcttgatgg tcatattaaa attttaaaag aaaatttagc tagaagaagc
      721 ataaatcaaa attgccaaaa ccttttacac agcctaaacc ttggagaaaa tatcgatact
      781 tgtatttaca aatttgaaag taatataaag gagattttgg acaaggatac ttatgaaaat
      841 gatgatgtaa atagtatagc agggaaggtg cttgattttt tagaaaacaa aaaagatatt
      901 ggatttaaat ttggtataaa attacttgat accactatag gaggtctttt taaaggagag
      961 ctaaccacaa tagcagctaa atcgggggtt gggaagactg ctttggcact tcaaataatg
     1021 ttaaattcat ttaagcaagg taaaaaaact ttatttataa gtagagaaat gaccagtgag
     1081 caagtattta tgagaaatat atgcagagta actggtgtaa gtacaaggaa tatgaaatct
     1141 aaagaaatag atgaaaatga ttggaaactt attgtaaatg ctataggtga tttaagcgaa
     1201 aataacttaa tttatattaa tgataagata gatacaatta gtgcaattag aaagaggata
     1261 aggcaggtaa agccagattt attaatagtg gattatgtgc aactccttac atctcagaaa
     1321 agcatggata agagagaaag ggaggtagct actttttcga gagaacttaa aaatatgact
     1381 ttagatttta atatacctgt aatccaatta agtcagttaa atgatgaaat gaaagattca
     1441 agaccttatg gagatagacc tatgagggat agcaaagcca tctaccacga tagcaacaat
     1501 gtggtttata tacatcaatt aaaggggagt gattatgaag aagcagtgag agatattgga
     1561 gaaagtgagg aggcagttag agcatcagaa tacaggggaa ttaagatggt tgatttaata
     1621 gttgcgaagt gtagagatgg acagactagg cataagcatt tttgttactt tggggataag
     1681 ttacattttc aagaactaaa ttattaggtc aataaagtag taataacaag cttattaaaa
     1741 gtcgggaaat agcaaataga ttataaaata tgaggtgaaa atatgttgga attaagaaaa
     1801 attatagaat tacttgaaga acacaagata gaagtaagta ttccaaagga gttacataag
     1861 tatagtttat tggaatacct tgaattgtgt caacaaagtg ggggaacata tttttatatt
     1921 aaaacactgg aaggggcttt aaaagaggct agaaatgagc ttatgattaa attgtttaat
     1981 gggaaaaatt atatggaatt agcgaggatg tttgaaatga gtgttattaa tgtcaggcat
     2041 attgttagaa aaaaatagat atttttggag aatacaagtg tgatatactt ctgaaataga
     2101 agttattata gttgaaataa aattgtggtt aataaattaa agttgtgtat aagtttaaga
     2161 ttatagagga gtataagtgt gattaacagc atgattttgt gaataaaatt gaggatatat
     2221 atgagttaaa tgtgtgatta ataaggtaag gttgtgtata agattatgag tatttaaaag
     2281 gaggggctat aattaatagt gtagagttgt gtgtttgctt aagattatat aataatttaa
     2341 tagattaaac tatactaata aggtaaaatc gtgtgtttag ttaaagctta ttataaaatc
     2401 ttgatatgtg attaatgatg caatatgtga ataaagttaa gattataata gatttaacct
     2461 gtggctaata gaataaaatt gtgaataaag ttaagattat aataggttta acctgtgatt
     2521 aatagagtaa gagtgtaaat agagataaac ttcaatatga gcttaaccta tgattaatag
     2581 agtaagagtg taaatagaga taaacttcaa tatgagctta acctatgatt aatagagtaa
     2641 gagtgtaaat agagataaac ttcaatatgg gtttaacttg tgattaatag agtagggtgt
     2701 aactaaagat aaacttcaat atgagtttaa cttgtgatta atagagtaag gtgcaactaa
     2761 agataaactt caatatgagc ttaacctatg attaatagag taagaatgta aatagagata
     2821 aacttcaata tgggcttaac ttgtggttaa tagagtaaga gtgtaactaa agataaactt
     2881 caatatgggt ttaacttgtg gttaatagag taagaatgta aatagagata aacttcaata
     2941 tgagcttaac ttgtgattaa tagagtaaga gtgtaaatag agataaactt caatatggga
     3001 ttaacttgtg attaatagag taagagtgta actaaagata aacttcaata tgagcttaac
     3061 ctatgattaa tagagtaaga atgtaaatag agataaactt caatatgggt ttaacctgtg
     3121 gttaatagag tagggtgtaa ctaaagataa acttcaatat gagtttaact tgtggctaat
     3181 agaataagat tgtgaataaa gttatagttt tagatgggta ttaacctgtt actaatagaa
     3241 tatgattagg tgtaaaatta tagagctgta taagaaaata ttatagatat attaaatatt
     3301 agataataaa tatttatata gatacacaaa agaaaaggcg atatgaagct aaacttaatg
     3361 acttcatatc gttttttatt tgaaactcct ttgtatttag agtgtgattt ttaatattga
     3421 ggttttatgt gattaagtca cagataaaaa gtaagtatct tgatataatt cttatagatt
     3481 aataatatag ggaggttatt atgtctaata aaaaaaagaa gtatgcaaat attttagcaa
     3541 atgagtgcgt tgcttgtggt agctgtataa aagcatgtcc aagaagtgct atctcagttc
     3601 catgtggcat tagtgcaaaa attaatagag atttatgtgt tggatgtggg atttgtgaaa
     3661 agatatgtcc agcatctgta attgagatta taacaatatt aaaagaggaa gaaggtaagt
     3721 gtcatgaata ataaaaaaca ttggtatgac tatttatgga ttttttcttc aatatatttg
     3781 atattgggat ttgtaaatat tattttcgct tggattgggc taatttgttt ctttgttcca
     3841 ctagctattt ctattgttaa gggaaataaa gcttactgta ataagtattg tggaagaggg
     3901 caactgttta atttgcttgg aaataaattg aagctgtcaa gaaatagaga tattcctaaa
     3961 tttattagaa gtaagtggtt tagatatggg tttttgacat tttttatggt aatgtttgcc
     4021 aatatgcttt tttcaactta tttagtgttt gaaggaagta gaaattttaa agaagtagtg
     4081 actttgttgt ggactataaa aatgccttgg caatgggcgt atcatggaac tttagtttca
     4141 ccttgggtgg tacagtttgc atttggtttt tatagtgtta tgcttacatc aactgttctt
     4201 ggtttgatta ctatgatatt gtttaaacct cgttcatggt gtgtttattg ccctatgggt
     4261 actatgactc agatgatatg taaggcaaag agtaagtcta tatagaaagg ctttatgtga
     4321 tatttacagt tttgttagag atgagtagta ggtttgaata tatatgacaa atatatgagt
     4381 ataggggcat agatttagtt tgtgtaaggt aaactgattt atgcttcttt tttatgtctt
     4441 agattattgg ctaagtggaa acaatgaatt tagatattaa gaattattca gttatttgaa
     4501 gaaaatagaa taataattaa cattatatga atggatattc attacatata agccagttaa
     4561 tagcaagatt tatttaatat gaaataggtc aagtatttaa actaaataag gtcatgacaa
     4621 ttaatataag tatagatata aaaagattag aagttataac ttataaaagg tattctatat
     4681 aaatagtatg tggaaaatag tcaaattaac ttattagaaa caaaaaatat acataaaaaa
     4741 ataaatgtaa atatcaaata agatactata taaaaataga tataagtatc ttttttatta
     4801 ttgaaattat gaaaatatat ttgatttcac acaaaaaaga gtgcctcatg aactaaaaag
     4861 ttcattttga gacacacttt tacagtgtca aagaacgaag tttatcctta tccaatactt
     4921 ttataccacc acgagataaa gacacaatac cttctctagc aaaatatttc aacattctcg
     4981 atacaacttc tctggcacta cccatatact tagcaatttg ctcatgagtc atatttatag
     5041 tgctactgcc atttttagct atctcatcaa ttaaaaaaga tgccaaacgc ttatcaaaac
     5101 tcataaaaag aatttgttgc atagcccaca taacatcaga aaaacgttcg gtagctaatt
     5161 tatatgaaaa taactctgca tgaatatttt ctgtagataa ttttgcaaaa acagaggaac
     5221 taatctgtat aatatcgcag tccgtttcag catctacata gacatcaaaa gttattgttt
     5281 ttagaataca ggaagctgat aaaatacata catctccatc atcaagtcgg aacagtgtaa
     5341 cctctcgacc ttcatctgaa agtatgtatg tacgtactgt tccagacttt ataatcaaaa
     5401 taccaataca gtcactatca ccacaatgta ctggattccc ttttttgtaa tgcgaggtat
     5461 ttgctgaaga aattaataat tctttttgta agtcagtaag tttatcccaa aaaggcaagc
     5521 tatgtgtaag tatatcgatt tcattttgtg aaatcataca atatctcctt tcttaaattg
     5581 ttataatccc atgatacaag tgaatagata aattgacaag tagtttgtta ataggattat
     5641 ttagtcagat agtaatagta tgatttatgt agaaaaaata tgataatata tattcttgct
     5701 attaatgaga aggtaatatg ttttgatgat agtaggtttg tataactaat gcataccgtt
     5761 tatttttaaa agattaagtt ccttatgtaa ccttatagac ataaaaccag caattatagc
     5821 agccatcaaa tcagctattg gttgtgcata taagatacca tttaacccat agaatacagg
     5881 aataatcaaa attataggga taaaacagac accttgtcta cacataccta aaaatccacc
     5941 ttcctttgct tttcctagtg cgaggaataa tgtagaatac actgtataga atccaaatag
     6001 cataaatgaa aacccatttg ccctaagagc tttactacca ttttctatta atagaatgtc
     6061 accacttgta aattgtgaaa ttatagattt tgggaagagt atcaatataa gaccaagcac
     6121 tacacaaaat attgtagtcc ataatatgga agtttttatt gctatatgta atctatcgta
     6181 gttctttgaa ccaaaattat aaccagcaat tggttggaaa ccttttataa aaccaaatac
     6241 aacaagacta cctaaagacg ttattcgtgt tacagcaccc attccagcta tcacagaatc
     6301 accatacttt gtagcctcca tattagttaa tgtaatggca agactagtca ataattgaaa
     6361 tattaaggta ggtatgccaa tttttaggat ttctgaaaaa atttcttttg aaaaacagca
     6421 ttcgttaatt ctaaaactaa atatactttt cttgcttaaa atatatccta cataaacaag
     6481 tgttgaagca atctgtgaga tagctgttgc aatagaagca ccccttaccc ccagaccaaa
     6541 agtataaatg aatattgggt ctaaaatcat attaagcaaa gcacctaata acatagcaca
     6601 catagttgtt ttagcagctc cttcacttgt gacaatatta gtcatagtga cattgaatat
     6661 attaaaaata gatgacacga cataaatgct agcataagat attgcataag gcataatact
     6721 ttttgtagct cctaacaatt ttaaaattgg attgagaaaa atcataatca caataataac
     6781 taatgtaccc actataatac tactgtaaat agatgtactt accacttttc ctgctttttc
     6841 attatcacca tggcctaaaa gtctggcaat atatgaagca gctccatttc caaacaataa
     6901 tcctaatccc acaacaagct gacctaatgg aaatgcaacg gatatagctc ccatttgact
     6961 tgttccaagt ccacccacaa agtatgcatc aaccaaattg tataatgcag ttatcatcat
     7021 acctatcatg gtaggtaatc ccatagataa caatgctttt gagattgggg cagaccctaa
     7081 taattcaatt cttttgttac tttctttcat acttaacact cctcttctat tgacattaag
     7141 tctaaattat aatactataa attatagtaa gtatggaaaa tagtactata atttatagta
     7201 tttgtcaata gtaagaaaac taaaggagat gatttttatg gctacaatag atttaattgt
     7261 attgggaata gtaaagcaac aacccaagag tgcttatgat attcaaaagg cagtggaata
     7321 caggaatata tcacgttggg ttaaaataag tacaccatca atttataaaa aagtaattca
     7381 attagaacaa aagggatata ttgaaagtaa aactgtgaag aatggaaaaa tgccagaaaa
     7441 agcagtttat tcattaactg aattaggtga gacacatttt ttagatttaa tgtttgatac
     7501 atctagcaaa ccaataaata ttcttttaga ttttaatgct gttatagtaa atttaaatag
     7561 ccttacacca gaaaaacaga aattatgtct caataatatt gaagagaatg ttcaagagtt
     7621 aaagaaaact attgaggaaa atataagtat aaagacagaa gtaccagaca cagcaaaagc
     7681 agtgttggag caacagttgg tgctagcaca agcattggaa acctggataa attctttaaa
     7741 agataatatg ggaaattaga attagagaag ctaacttaat aatcattaaa caaaaaccag
     7801 catatttaat aatgagatat tagaaaaaat aaaacaaatg atataaactg aaaagtatat
     7861 ttaaaataaa gctcacttaa ttgagggaga atgttaaact ttt
//
LOCUS       DBGJFU010000156         7897 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.159, whole genome shotgun sequence.
ACCESSION   DBGJFU010000156 DBGJFU010000000
VERSION     DBGJFU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.159"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            27..101
                     /locus_tag="ACOIAS_003324"
     tRNA            27..101
                     /locus_tag="ACOIAS_003324"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="ACOIAS_003325"
     tRNA            116..200
                     /locus_tag="ACOIAS_003325"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="ACOIAS_003326"
     tRNA            210..293
                     /locus_tag="ACOIAS_003326"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="ACOIAS_003327"
     tRNA            317..391
                     /locus_tag="ACOIAS_003327"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="ACOIAS_003328"
     tRNA            416..492
                     /locus_tag="ACOIAS_003328"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="ACOIAS_003329"
     tRNA            502..577
                     /locus_tag="ACOIAS_003329"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="ACOIAS_003330"
     tRNA            586..661
                     /locus_tag="ACOIAS_003330"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="ACOIAS_003331"
     tRNA            664..752
                     /locus_tag="ACOIAS_003331"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="ACOIAS_003332"
     tRNA            756..831
                     /locus_tag="ACOIAS_003332"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="ACOIAS_003333"
     tRNA            838..911
                     /locus_tag="ACOIAS_003333"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="ACOIAS_003334"
     tRNA            926..1002
                     /locus_tag="ACOIAS_003334"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="ACOIAS_003335"
     tRNA            1020..1096
                     /locus_tag="ACOIAS_003335"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="ACOIAS_003336"
     tRNA            1105..1180
                     /locus_tag="ACOIAS_003336"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="ACOIAS_003337"
     tRNA            1188..1261
                     /locus_tag="ACOIAS_003337"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="ACOIAS_003338"
     tRNA            1269..1345
                     /locus_tag="ACOIAS_003338"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="ACOIAS_003339"
     tRNA            1358..1433
                     /locus_tag="ACOIAS_003339"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="ACOIAS_003340"
     CDS             1509..1922
                     /locus_tag="ACOIAS_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703011.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="ACOIAS_003341"
     CDS             2231..5662
                     /locus_tag="ACOIAS_003341"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HGZ2703012.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="ACOIAS_003342"
     CDS             5904..7832
                     /locus_tag="ACOIAS_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGZ2703013.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3094 a         1011 c         1587 g         2205 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctgta aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag ataatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactag
//
LOCUS       DBGJFU010000157         7847 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.160, whole genome shotgun sequence.
ACCESSION   DBGJFU010000157 DBGJFU010000000
VERSION     DBGJFU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.160"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            44..1495
                     /locus_tag="ACOIAS_003343"
     CDS             44..1495
                     /locus_tag="ACOIAS_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGZ2703014.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1693..2346)
                     /locus_tag="ACOIAS_003344"
     CDS             complement(1693..2346)
                     /locus_tag="ACOIAS_003344"
                     /inference="COORDINATES: protein motif:HMM:NF019947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HGZ2703015.1"
                     /translation="MTFNMTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSIN
                     KIANGKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGV
                     DNKIIGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKV
                     IVENDSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            2808..4187
                     /locus_tag="ACOIAS_003345"
     CDS             2808..4187
                     /locus_tag="ACOIAS_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2703016.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFINLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            4231..4965
                     /locus_tag="ACOIAS_003346"
     CDS             4231..4965
                     /locus_tag="ACOIAS_003346"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF009022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGZ2703017.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            5054..6253
                     /locus_tag="ACOIAS_003347"
     CDS             5054..6253
                     /locus_tag="ACOIAS_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGZ2703018.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAEKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            6277..6759
                     /locus_tag="ACOIAS_003348"
     CDS             6277..6759
                     /locus_tag="ACOIAS_003348"
                     /inference="COORDINATES: protein motif:HMM:NF012578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGZ2703019.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(7074..7202)
                     /locus_tag="ACOIAS_003349"
     CDS             complement(7074..7202)
                     /locus_tag="ACOIAS_003349"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGZ2703020.1"
                     /translation="MYSIIFIYAVHYKVKIDGKIINKAAYTAICMNLEGVKDVLGI"
     gene            complement(7300..7599)
                     /locus_tag="ACOIAS_003350"
     CDS             complement(7300..7599)
                     /locus_tag="ACOIAS_003350"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HGZ2703021.1"
                     /translation="MLKDLFASTIHKMLESEIEDHLMYERYDNQSKATSNSRNGYRAK
                     NVKSDFGEVKLNIPRDDFQPRVIQNYENEISGIENQVIGMYSKGMSTRDIYHTFK"
BASE COUNT         2898 a          905 c         1343 g         2701 t
ORIGIN      
        1 atttattgag aaaacaaaac taaatagggg ggtaattaat ataatggatt ttttcaatgg
       61 tattttaacg agtattaaca atgcagtagt atttgtaaac aattacttat ggtcgtatat
      121 cttaattata atgttagttg taattggtat ttactttact attaaaacaa attttgtaca
      181 attcagatat tttatagaaa tgtttagact tctgggtgat ggtacagcga ataaaaatgc
      241 aaaaaaggaa ggaaaaatct cttcatttca agcattttgt ataagtacat cttcaagagt
      301 tggaactgga aatatagctg gaatagcaat tgcagtggta gcaggaggac ctggtgctgt
      361 attttggatg tggcttatag cattaattgg ttctgcatca agctttgttg agagtacatt
      421 agctcagatt tacaaagtga aagatggtca agcttttaga ggtggaccag cgtattatat
      481 ggaacaaggt ctaaataaaa aatggatggg tattttattc tcaatactta taactatatg
      541 ttatggtttt gtatttaatg cagttcaagc aaatactgtt tcacttgcat ttaataatgc
      601 atttggaata agtaaaatgg ctatgggaat aggtcttgga attttaactg caatagttat
      661 ttttggtgga gtgcatagag ttgcaaaagt ttctgaaata atagttcctg tatttgcagg
      721 attatatata ttagttgctt tggctatagt tgtaatgaat ataacagaga taccaagtgt
      781 tatagcattg atagttgaaa gtgcatttga ttttaaagga atggcgattg gtacatttat
      841 gggagttgta atgactggag tgaagagagg tctattttct aatgaagcag gtatgggtag
      901 tgctcctaat gctgctgcaa cagcacatgt aactcatcct gtaaaacaag gtttaattca
      961 atctttaggt gtttttacag atactataat aatatgtagt tgtacagcat tcatggtatt
     1021 attatattca ggttatagta gtagtggagc aactggaatt gaattagctc aagaagcttt
     1081 aactcatcat ataggaccag ttggaaatat atttatagca gtttgtatat tcttgtttgc
     1141 atttagttct atagttggta attactatta tggtgaatca aatatggagt ttatgagtgg
     1201 aagtaagact aaacttaatg tatttagagt ttttgttgta ggtatggttt tatttggttc
     1261 acttactcaa gtggatgtag tttggaatct tgcagactta tttatggcat tgatggcgat
     1321 aataaactta atagcaatag cacttttagg aaaatatgca tttattgctt taaaagatta
     1381 tacaagtcaa aaaaaatcag gaataaaaga ccctgtgttt attgcagatg aaatagaagg
     1441 cctagaaaag gtatcagagt ggcataggga attatctgag gaacatttag gataaaaaat
     1501 agtataaaat tataaaacca ttttgaaaga ctagagtatg tatatacttt atatgaatat
     1561 aatattgaat taatttatat tactggaatt tttaattcca gtaatatttt tttaattaca
     1621 aggatattat gatgacttaa agactgtgga aaactctttt ttagttttca ttattaatat
     1681 taattgacaa atttagtttt tatttaaatt tcttaagtgc atataaatcg tattttttga
     1741 taatcccatt aagtctgcaa ctttaacaac agaatcctta attttaaaaa taccactttc
     1801 atttaaaata gaaacaatag atttattttt atcattgcta gatatattag aatcattttc
     1861 aacaattacc tttgcatctt ggtatgattt taaaattact tcatcagtat cttctacgat
     1921 attttctaaa acaggagcac tttgcatagc attatgttcc ggaatatatg aaccaattat
     1981 tcttgttagt ggtgtattaa gataaaaatg tatgcagagt agaccgatta ttttattatc
     2041 aactcccctt atcactatat ctgttgattt taaaggttca cctagtttgt ttctattaaa
     2101 ataactgata aaatctccat cagcatcatt ttcgagttta ttaatgagtg ccaaatcaaa
     2161 atcagttata atatctcctt cttttacagt attatattta ccattagcta ttttatttat
     2221 tgaatagtgt actccattaa atttatataa aataaactcg tatccttctc ctaaatacgc
     2281 agataatcca tccactagat tgttataaga atcgaaaata tagttatcag tcatgttaaa
     2341 agtcatatgt tacctcctaa gaaatatctt aatatcatat taacatataa aatgaattta
     2401 ataaaatata tttcagtgaa ataataaata taatgaaaaa aaaactattg actgaaaaat
     2461 atttcagtga tattattaaa atacagtaag gcaaacgtat tattgacaga atatataaaa
     2521 tgttaaaaat agtatctagg aattaaacaa attaaaggac tagtgatttg aattttttta
     2581 gtataatatg tgacttccaa ttaatattta ttaaacacca ataaaattgt aaatatgctt
     2641 aatgatatac tttagcgatg aagtgaaaat attttatttt tatgattaac ctataggaat
     2701 taatttagca tactgtttag ctagtaaatc taaataaatt ttagctttag ctgatatcat
     2761 gttgaataaa gtttctattg gattttgtat aacaaaggag tggggagatg gcacaagata
     2821 aaattgtggt tgaaatttta gaaaacattg gtggtaagga aaatattaag gaagtagcac
     2881 attgtatgac tagactgaga ataaaggtat gtgatagttc tcaggtaaat caggaaaaac
     2941 taaagaaaat aaaaggtgta ctaggtcttg tattaaaagg tgagcaatat caaattgtta
     3001 taggtcctaa tgtatcacaa gtgtataata ctcttaatga agttatagga attatacctg
     3061 agaaggatag taaaaaaaca atatctgaag gtaaacaaaa tagtattgca aaaatattag
     3121 atactattgc aggtattttt aatcctatag ttccagcatt agcgggtgca ggtctaataa
     3181 aagctatatt agcattacta gtagcatcaa agtttttatc tgcagatagt gaaacctata
     3241 taatattaaa tacaatatct gatggtgtat tttcattttt accatttatg ttagcttact
     3301 cagcagctaa aatatttaaa atgaatactt atgttgctgt agcaatagca ggagcaatgc
     3361 tacatcctaa tttcattaat ttaattcaac aaggcttaac taaaatatct tttatcggta
     3421 ttccgatgtc actaatccag tataacggaa gtgtatttcc gatagtacta actatttggt
     3481 ttgcatcgta tatagaaaaa ggagtagata aaataacacc aaaggcactt aagataatat
     3541 tagtacctgc attgactatt ttatttgcag ctcctatagc attaattatt attggaccaa
     3601 tagctcaaat gctaggaaac tatatagccg gtggagcaag tatcctattt gaaagaggag
     3661 gaatatttgc agggttaatt tacggaggaa tttattcatt catggtagta acaggattac
     3721 atcatggtat ggttcctgta ttagtagatg gaataacaag atatggattt aactatataa
     3781 gtcctgtgtc aggtagttca aatatggctc aagcaggatc tgcatttgga gtatggttaa
     3841 aagcaaaaga taaagatttg aaggctgtag cagctacagc taccatatca gcattaactg
     3901 gagttaccga acctgcaata tatggtgtta atttaagatt aaagaaaccg tttttatgcg
     3961 ctgctatagg tggtgcagca ggaggtggat ttgctgcata tttccaatgt aaagcatttg
     4021 ctatgggagg tccatcgttt ttaactcttc ctatgtttat aggagaaggt ggacatgtag
     4081 gattggtagc aatagcattt acaatagcat ttatagtatc tgcaatagct acctatatag
     4141 taggatttga agaagaggta acagcagatt cacacgctaa cttataataa agagaaatta
     4201 tttaacatat caaaaaggag attaattact atgaaaattt atttaactaa agattatgaa
     4261 gaaatgagcc aagtggcagc acaaatggtg ttaaagtata tgtacagcaa tgataataaa
     4321 agaataaacc tttctataac agcaggtcaa acaccaaaaa gaacatacga aatattaagt
     4381 gaatttgtta agaataaacc ttattttgaa aatgtacatt attataactt tgatgaaata
     4441 ccaataaaag gcgaaaaagt tggagttact ataagaaatt taactaatat gtactttaga
     4501 gaagctcaaa taccagaaga aagaatacat gtgttagatg aaaataatta tcaaggcttt
     4561 gatagaaaga tagaagccga tggtggaata gacatggtat taatgggatt aggttgggat
     4621 ggacacttct gtggaaatct tagtggtaca gtcaactctt ttgatgaaga gacaagagct
     4681 gtttcaactt atataacaga tgatatggct aaactattaa caaaatctgt aggtggaaat
     4741 ccagaaaaat tatttgatta ctatgtgaca tatggaccta aaacaataat gcattgtaaa
     4801 aatataataa tgatagtaaa cggaaaacac aaagctcaaa tattaaagca aacactattt
     4861 ggaccattag atttattatg tccatcatct ttattgagat tacatcctaa cttcacatta
     4921 atagtagatg aagaagcagc atctgaaata aaagatttaa tataaataag taaggaatta
     4981 aaattaaaaa tgaatttagg tgcaaagtaa tattttattt tgcacctttg ctttaataaa
     5041 aaggagaata aaaatgtata atcatatgaa aaaagtattc caaggattag agggtgggtt
     5101 attttcaagt gtagagaaag ctgatgtagg cgattcttat caaaaattag gtgagcaggg
     5161 tgtaagttta atgggatggg cagatccatt tttcccagat tactgtatac ctagacatat
     5221 attagatgca gctacattag cgatatcaag taatatgtca actcattata cagcgcctgt
     5281 aggtaatgac ttattaaaaa ttgagatagc agaaaaatta aaagaagtaa ataatttaga
     5341 tgttgatttt aaaagaaata tattaataac tcctgggtca gactcagggc tttattactc
     5401 aatgttaccg tttataaatg aaggggatga ggttcttata ccaacaccat gctatccaaa
     5461 taatatacaa aatactaaat taatgggagg agttcctata tttgtagagt taaaggaatc
     5521 agatggatat caaatagata taacagaatt tgagaagaga ttaactaata agacgaagtt
     5581 agtagttctt actcatccta ataatccaac aactacaata tttaataaag aatctttaga
     5641 aaaattagct aaatttatta tagacaatga tttaatatta atatgtgatc aagcatttga
     5701 agattttact tttgaaaatg aattaataac accagcttca ttagaaggta tgtttgaaag
     5761 aacagtaaca atattttcaa catcaaaagg tatgggatta agtggattta gagttgcata
     5821 tatggtatgc agcgataaat taatggacaa atactatgga gcagcagtga cagtattagg
     5881 tgctactaat acagtagctc aaatgtcaac gattgaagca tttaaagata gaagctttat
     5941 ggaggatttt gaaagagttt ttgataaaag aaggcaagaa gcatataaga taatcaatga
     6001 aatacctaat gtatctatgc agatgcctga gtccggattt ttaggttgga taaatgtatc
     6061 aaagcttgga acatcatcag aaatacaaaa gtatctagtt gacgaggcta aagtttgtat
     6121 taatgatgga attaattatg gtccaggagg agaagggcat ttgagaataa ttttaggaag
     6181 ctataaagac gatagtaagg taattggtgc attaaataga ataaaagatg ctttaattaa
     6241 aaagcaacaa taaataaaaa ataattggag tggagtatgt tatcaatatt taagagtaaa
     6301 aaaaatgaca acaagcttaa ggcatttgca tcagggaaag taattcctat agaagatgtt
     6361 gatgataatg ttttttcatc taaaatgtta ggtgatggaa tcgctattta tccaactaat
     6421 gaatatgttg tagccccttg tgatgcagaa gtaaatataa taatgaaaga tactaaacat
     6481 gcagttggaa ttaaattatt taatggtatg gaaatcttat ttcatgttgg aatagatact
     6541 gtatctatga atggagaagg ttttgagata tttgtttcac caggtgacaa gataaaaaga
     6601 ggtgataaat taatatcttt taatagtaaa ttaataaaag aagaagggtt acaagatgta
     6661 gttataatgg tagttactaa ttcagaacag tataaaaata taaaatatca tattggactg
     6721 gaggcagtaa tcgatgatac tattattgca gaaatttaaa aacagttatt taagtaaata
     6781 cttagataat ttagatatag ttaaaattaa atccttaaaa tcaaattatt taatttttgt
     6841 gtaattttaa ctataaagcc gattaataat ataaagtata ctaaataata tggttattat
     6901 gattgttact gacaaagcct aagtagatat aacaatttgc tctaattgag tattgctata
     6961 tctacttgtt tttatattta aaataaaaga gtctgcattg aaaagataat tctgatgaga
     7021 gtaaataggg tatttaataa tagtttaatt ctgacttatc aagatgaacc tattcatata
     7081 cctaaaacat ctttaacacc ttctaaattc atacaaatag ctgtataggc tgccttattg
     7141 attatttttc cgtcaatttt tactttgtag tgaacagcat atataaatat tatcgaatag
     7201 attgtatcaa gagctctatt ttgctattca ttagctaaag atagtactct gtcagttatt
     7261 tttgaaacta atgcatgaga tatatcaatt ccgtaaatat tatttaaatg tatgatatat
     7321 atcgcgagtc gacattcctt ttgagtacat gccaattact tggttctcta ttccagaaat
     7381 ttcattttcg taattttgaa taaccctagg ctggaaatca tccctaggga tatttaattt
     7441 tacctctcca aaatctgatt taacattctt agctctatat ccatttcttg aatttgatgt
     7501 tgctttactt tggttatcat atctctcgta cattaaatga tcttcaatct ctgactctaa
     7561 cattttgtga attgtgcttg cgaataaatc ttttaacatc tcttggatgt ctttagtatt
     7621 ttttacatca tactcttgaa ctaagtgttt taatatttaa tttttgttgt acataaaaaa
     7681 tgtgtacctc ctattttggt aattagattt aatatcttga cttttaggag aatacacatt
     7741 ttattttaca gaccctcttt tctagaaaag tttttatgaa aattgtgata taaattatta
     7801 ataaaaaata attttatatt tattttatta tgtaatttag ttttatt
//
LOCUS       DBGJFU010000158         7771 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.161, whole genome shotgun sequence.
ACCESSION   DBGJFU010000158 DBGJFU010000000
VERSION     DBGJFU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.161"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            340..1161
                     /gene="yidA"
                     /locus_tag="ACOIAS_003351"
     CDS             340..1161
                     /gene="yidA"
                     /locus_tag="ACOIAS_003351"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGZ2703022.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1298..1939)
                     /gene="saoL"
                     /locus_tag="ACOIAS_003352"
     CDS             complement(1298..1939)
                     /gene="saoL"
                     /locus_tag="ACOIAS_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_238476746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1454..1456),aa:Sec)
                     /transl_table=11
                     /product="MerB-like organometallic lyase SaoL"
                     /protein_id="HGZ2703023.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     CUNIMNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLKQKVLS"
     gene            complement(1952..2704)
                     /gene="saoA"
                     /locus_tag="ACOIAS_003353"
     CDS             complement(1952..2704)
                     /gene="saoA"
                     /locus_tag="ACOIAS_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein SaoA"
                     /protein_id="HGZ2703024.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2704..3447)
                     /gene="saoP"
                     /locus_tag="ACOIAS_003354"
     CDS             complement(2704..3447)
                     /gene="saoP"
                     /locus_tag="ACOIAS_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591060.1"
                     /note="Most members of this family are selenoproteins with
                     the selenocysteine residue at the channel-gating position;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2881..2883),aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease subunit SaoP"
                     /protein_id="HGZ2703025.1"
                     /translation="MDILYKIILLVIIFLVWHFAAKDIGSSLLLPMPVDVIKGFFFCV
                     TDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFSKTFERVLSPVVDSVRQVPIMA
                     WVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVRAISKDYYNAARSMGASPIVIF
                     TNVIVPASLPDILTGSRIAISTGWMSVIUAEFIATSAGLGYSMVQAQTLMHTDVLIGL
                     MIFAALIGFIIDRVLKFINKILCRWRFAD"
     gene            complement(3471..4259)
                     /gene="saoB"
                     /locus_tag="ACOIAS_003355"
     CDS             complement(3471..4259)
                     /gene="saoB"
                     /locus_tag="ACOIAS_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4044..4046),aa:Sec)
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     (seleno)protein SaoB"
                     /protein_id="HGZ2703026.1"
                     /translation="MKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTSGFV
                     INYMVNRDYFENIKVSDIMDIFTINDCUASTMQWALSSEEIDIAIICKDAAKQYVNVN
                     SGFEIVGTVVQNSDIFLLSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAY
                     GLESNLVDGVVIDCIKSIGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYN
                     KSVDALKNKATFKKALKEYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(4273..5364)
                     /gene="saoX"
                     /locus_tag="ACOIAS_003356"
     CDS             complement(4273..5364)
                     /gene="saoX"
                     /locus_tag="ACOIAS_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HGZ2703027.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5399..5857)
                     /gene="saoC"
                     /locus_tag="ACOIAS_003357"
     CDS             complement(5399..5857)
                     /gene="saoC"
                     /locus_tag="ACOIAS_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Cys-COOH (seleno)protein SaoC"
                     /protein_id="HGZ2703028.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5850..6935)
                     /gene="saoE"
                     /locus_tag="ACOIAS_003358"
     CDS             complement(5850..6935)
                     /gene="saoE"
                     /locus_tag="ACOIAS_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter SaoE"
                     /protein_id="HGZ2703029.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(6962..7219)
                     /gene="saoT"
                     /locus_tag="ACOIAS_003359"
     CDS             complement(6962..7219)
                     /gene="saoT"
                     /locus_tag="ACOIAS_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_240067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7184..7186),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HGZ2703030.1"
                     /translation="MSKILVEFINTUPSCDEHGVFIKKMSEKYADKLEIKLYQAGKDF
                     SYIKKYGIITKGTLIINQKKKYDRLNKDTIERAIVEAINNN"
     gene            complement(7309..7641)
                     /gene="saoD"
                     /locus_tag="ACOIAS_003360"
     CDS             complement(7309..7641)
                     /gene="saoD"
                     /locus_tag="ACOIAS_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE-related protein SaoD"
                     /protein_id="HGZ2703031.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
BASE COUNT         2557 a         1362 c          859 g         2993 t
ORIGIN      
        1 attttataac acaaaactaa ctaatataac aaattaccat cagtctattt attcaaactt
       61 ctaactatat aatatacatt tatttatatt aacaatgcaa acgataccat tatttgtaat
      121 taagaatttt ggaattattg attacaaagt ttttacattc tcatctcaca taagtaatat
      181 ttttatcaaa aaattaatat tattaaactt tctaaattac ttatctcttg atataatagt
      241 atacaaaaca aaccatattt cccactgaaa ctttgttcaa cactagctgt gtcaacgttt
      301 tcacaggaaa tttgagtata taatttattg gaggttgata tgtacaaatt aatcgcacta
      361 gacattgatg gaacaatatt aaatacacaa aaaaggatta ctcctgaagt ctttgagtcc
      421 attcaggagg caaaaagggc tggggcaaaa gtagttataa caacaggcag acctcttcct
      481 ggtgtaaaag aacttctaaa tcaactaaat ttaacagatg aaggtgatta tgttatatgc
      541 tttaatggag caattattca agaagttaaa agtgagaaaa tcatacatga tgtagaaatg
      601 agtttagatg attttgattt tatatataat aacgtttgta aaaaatataa gactaaaata
      661 cacataaata caatgacaaa tttaattaca ccaaatgaaa cgcctggcaa atatacactt
      721 catgaagcga aattaaataa tatagaagtc aaatatatac aaaaagataa aattgatgaa
      781 tctattaaaa tctgtaaaat tatgatagtt gatgaaccag aaagattgga agaaatcata
      841 cagcaactac ctaaaaattt attcaataaa tatactatag taaggtctgc tcctttttat
      901 ctagaatttt taggtaaaac tactaataaa ggaactgctc ttaaaacatt atgtacgaat
      961 ctaaatatac ctatagaaaa tgctattgct gttggcgatg aagaaaatga ccaacatatg
     1021 ataaagtatg caggtcttgg tgtggctatg gggaatgcac gtaatagtat aaaggaaatt
     1081 gcagattatg ttactgatac taataatgaa aatggagttg caaaggttat aaataaatat
     1141 atacttaata aggctatcta atcatctaaa aaaaactaaa aatatacctt tagatttaaa
     1201 tacttttcac atccctactc tatactaatt tatatagtat ggatattacc tatcattttt
     1261 atctaaaggt catttttagt ttttcatata tgtatatcta tgatagtacc ttttgtttga
     1321 gtttaaatat tttatatgat tctactattg cctcattaat atctaaacaa tatatatctt
     1381 catcattaga taatcccgct tcgtttgtcc aagcatcaaa atcactcttc gtccagaaga
     1441 aattcattat atttcagcaa ctagcagccc aatttagatg cttatcaaga tttatatgaa
     1501 gcactcttaa gtttggatta ttcacatact caatcttttc attttttata attacttcta
     1561 tttccttacc tgtagcacta caaatagagt ttatttttat atcttggttg aatgtgcaag
     1621 aggttcctat agcatctata gcacacattg cactgaaact tctattatct ctcaaagtta
     1681 ctttatgatt tgttggcaat gctgatacag gatatataaa atttatacta ttcccatcaa
     1741 caaccattat atttttataa ataaaatatt gtagtgtcct ttctatatat tctttttcta
     1801 tattcaactt tttacttata tattcagtca cttcttgaag tgtaacacta gaacctttat
     1861 ctattatcat atccataata gagattctta ttttctgttg tgtattatcc aaactattgt
     1921 atttttctat ttgactcatg tctactcctc tttaaacttc ataacacttt gataaagtat
     1981 cagttaattt gtttttgtac tcttcatatt tttggcttcc tctaattcta ggtcttggaa
     2041 tatctatatt tatatcatca ataattcttc ctttatctct cgacattact ataactctat
     2101 cactcatata tacagcttca tcaacatcat gagttaccat taatgcagtt attttcttct
     2161 ttatccatat tcgttctagc tcttcctgta aattttgtct catttgaaaa tctacagctc
     2221 ctaatggttc atccattagt aatacctctg gattacatgc taaagctctt atcatggcca
     2281 ctctttgttt cattcctcca gataattgat gcggaaacag tttttctttt cctttcaagt
     2341 ctgacataac caaaagttct tctagcattt cttctctttt ttcttttggt attttctgct
     2401 gtttcatagg aaatagaaca ttacctctta tagttttcca aggaaataaa gcataatttt
     2461 gaaatataaa agctctatct atgcccggtt ttttaactct tttatcattt ataaaaacat
     2521 ctccacgttc tgatttttga aacccaccta ttatagtaag taaagttgtt tttccacaac
     2581 ctgatggtcc taaaagacag acaaattgtc catcatctat atccatactt atatcttcta
     2641 aaacctttgt atgtactcta ttatttacaa atgtcttatt tatatctctt acacttaact
     2701 tcattaatct gcaaacctcc atctacaaag gattttattt ataaacttta ataccctatc
     2761 tattataaat cctattaaag ctgcaaatat cataagccca attaatacat ctgtatgcat
     2821 taatgtttga gcttgtacca ttgagtaacc gagaccggca ctcgtagcta taaactcggc
     2881 tcagataaca gacatccatc ctgtacttat agcaatcctt gaaccagtta gtatatctgg
     2941 aagagatgcg ggaacaatta catttgtaaa tatcacaatt ggcgatgctc ccatacttct
     3001 agctgcatta taataatcct ttgatattgc tcttactcct tgaatagtat ttaaaattat
     3061 agggaaaacc cctgaaaaag ctattaaaaa tattgttgga ccatctccta taccaaacca
     3121 tactatagta agaggaaccc atgccattat tggaacttgc cttacagaat ctactactgg
     3181 agataaaacc ctttcaaaag ttttagaaaa tcccataata aatcctattg gaagcccaaa
     3241 caataatgca tacataaaac ctttaagtac tctctgcaat gttataaaaa gattagtgac
     3301 tgtctctgca tctgtcacac agaaaaagaa accttttatt acatctactg gcattggaag
     3361 taacaaagaa ctccctatat cttttgctgc aaaatgccag actaaaaata taataactaa
     3421 tagtattatt ttatataaaa tatccatact accatcagtt tttttcttag ttaatctatc
     3481 gcttttatct ttagaaattt taatttccaa ttgcttattt cctccatatc tatatttctt
     3541 atattcctgt actcttttaa tgctttctta aatgtagctt tattctttaa agcatcaaca
     3601 cttttattgt aaagtgttat aaaatcacta tatgctttac tttttttaaa ctctttactt
     3661 acaactaata cataggtatc ataatctcct aatttagttg tagattttct tttgccctct
     3721 aacccaattg actttatgca atctattaca acaccatcta ctaaattact ttctaatcca
     3781 tatgctagtg cagtccctaa taagggagct acttccacat tcttgtagta ttttttaacc
     3841 aattctgact ggtaatctct attttgaatg actcctatct tttcaggatt attatcactt
     3901 aatagaaata tatcagaatt ttgaactaca gtaccaacaa tttcaaatcc actatttaca
     3961 tttacatatt gttttgcagc atctttacat atgattgcta tatctatttc ctctgaactc
     4021 agagcccact gcattgtact tgctcagcaa tcattaattg taaaaatatc cattatatca
     4081 cttactttta tattttcaaa gtagtctcta tttaccatat aatttattac aaaaccagag
     4141 gtatcatcag atacccctat tcttaatttt ccttttattt cttctttatc ttcaaaatta
     4201 aaaaatatta taaatatact tcccaaaata atgataaaaa gtattattat atttttcata
     4261 ttctccattt atttataata cctttgcttt ccagtcttca taactcatac cttcaggaaa
     4321 ctctgggtta actttttctt ttataaattt gtcaaaatca tcagctccac aagagtccaa
     4381 taagcttgta tcaatcgtat cttcatattt aggtatatca tctaaagcct ttaaatcttt
     4441 atacatttgt aaattatttt tatattcttc acctgtaact ttccaagtaa gagttctacc
     4501 ttctccaaca gtcttcttat agatagttct aagtgcaact tcttcttcta cattataata
     4561 ttttgcaaat attttagctg cttcagctgg atttgtatat atatattcta ttgcttttgt
     4621 atgtgctaga accaattttt ttgctaaatc tggatgttct tttataaagt ttttgtttaa
     4681 tgagaatgaa caacagttat attctttgcc attagctttt tctttatatt gagtagatgc
     4741 tattattttt cctgcaccgt cattttcagc tacagaaccc caagggtcac aagatgtaaa
     4801 agcttgaatt ttcccagttt taagagctaa atatttatct ttgtctgaat ccatatttac
     4861 aacttcatat tttgatgggt cagttggtag acctgtttct ggaccataat cacttgtcca
     4921 taagaaatca ctcatatctg ttgcaatttt tttacctatt aaatctttag gttcttttat
     4981 atcttttgaa actattaaat attcagaacc accagtatgg ttgttagctg ctattgttat
     5041 tggtgaaccc ttaggtattg ctccaactaa ccccttagtt cctatgtaac ctgcatccat
     5101 ttttcctgct gccatagctt caggaacttt accatttcca acagttttta cattaagacc
     5161 taaatcttta tatattcctg ccgattctgc tactggtccc gcagtcatat ggtcacaatt
     5221 ataataacct actgttattg tataatcatc ttttccagtt tttgcttctt ctttttcttt
     5281 tggtttacta ctacatcctg ttaatataga tgtacccatt acaccaaata agattaatgc
     5341 ttgaatcttc ttagataatt tcatataaat ttcccacctt tatgtattat ttttattttt
     5401 agcaacattt gtccatatct tcgccaaata aatcaacaaa ttttttaccc tctactctgt
     5461 aaatagcata tcctacatta ccatttttag accctgatac aattacttct attggtgctt
     5521 tatcatcaat atttttaaag gttatagttt gattctctat aggtgctggt attggctttg
     5581 ttatatagtg gctattttta tcagaaacta cgactaccat ttcatttgaa ttattgcttt
     5641 ttttatatat aactactaaa tcttttttcc catcattatt taagtcttct tcagcacaag
     5701 ttattatgtc ttctttataa ttttttctaa aatattctaa tgtttcttta ttcacatttg
     5761 gaatagaagc atcttctttg atgttatcat caactatttt tgataaaatg attaacacaa
     5821 ctactatgca ggatattata actatctttt taagcatgat ttcctatgac ctctttcatc
     5881 tttggtatta ttgatttaaa tgcaaataaa ataattaaaa tagtacatac atactcaaaa
     5941 ctatcaggta tatttaacat tagtttattt gccaactgta ttgaagaatt tgttgcatct
     6001 aaatttactg agttattatt cactaaaagt aaatttgtta tatatccaat tattattgaa
     6061 gacagtgtca aagttacaga atatataata acagttcttt ttccaataag tttaaacata
     6121 cttataagtt ctggtagatt tgtagctgct ccagctatta gaaatgttat cgctatacca
     6181 ggagatgctc cacttgcaac aagggctgct ataaacggta tatgtcctac agcacataca
     6241 tacataacac atgcaagtat tgctatactt ccaagagata gcaaacctgg atttcctaaa
     6301 tatttttgaa ttatactatt tggaaaaata gttgttataa atcctgcaaa aaccataccc
     6361 attactacat atttactaat agtaagggct aattcaccaa aagaccattt aagaccatca
     6421 atcatatttt ccataaaact tcttttttct tcttctaatt ctattacttc ttcctctgaa
     6481 tcattatgaa gtgataattc ttcttttcca aaacaatttc ctaaaaatcc tactaaaaat
     6541 ggtactaaaa aaccagctat tagataaatg attgtaactt taggacctaa aagacctaga
     6601 caaagaacta cagctattgg atttattatt ggagttgatg tcataaaagc aagtactggt
     6661 cctaaatatg ctcctgaata atacatactt attccaagtg gtattactcc acaactacat
     6721 attggtaaaa acattcctga gatagtactt ttaattaaag aagaaaattt cttatttcca
     6781 agttgttttt gaaaccttct tggagttaaa acattgtgaa gtacacctgc caagataaaa
     6841 cttatcataa gccatcctga tgctccattc agtgtaacta tagaagctac tatagtatct
     6901 ttaactccat ttaaaacaat ttctaatata ttcatttttg atactcctaa tttttaaact
     6961 tttaattatt gtttatagct tctactattg ccctttcaat tgtatcttta tttaatctat
     7021 catatttctt tttctgattt attattaaag ttccttttgt tataatccca tattttttta
     7081 tatacgaaaa atctttccct gcttgatata attttatttc caatttatca gcatactttt
     7141 ctgacatttt ttttataaac actccatgtt catcacaaga tggtcaagtg tttataaatt
     7201 caactaaaat tttagacatt tgttcatccc ctactttaaa caatttatct tttacttaaa
     7261 ataatccaca gaaaataaat ttttctatgg attgtatcct atactaattt ataacgttat
     7321 aacttggtcc acacctgcac tgcctaaaca agcatatagg tttgggaaac atccaattgt
     7381 agctccatca accaaattat tctctatacc tctttctata gcacattggt cacaagccat
     7441 tattatcata tcagtttttt catgtaattt acttagtctc tcgcctacag gattaccttt
     7501 agataaaaat aaggtattat ccataaagaa aaacatccct agaacgtcta ctccatgagt
     7561 tccttgttca agttgtggta ttatcatttt gtctagaatt ttatgtgtat tagtactact
     7621 aaaaatataa gctactttca tttttattcc ccctatactt tttatatttt acaaaatttt
     7681 acagatacaa ttatattata agtttattct tatttatctg taaaatagat attaactata
     7741 ttattaaaag tgtatatagt atttatctat t
//
LOCUS       DBGJFU010000159         7694 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.162, whole genome shotgun sequence.
ACCESSION   DBGJFU010000159 DBGJFU010000000
VERSION     DBGJFU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.162"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            321..1514
                     /gene="nifV"
                     /locus_tag="ACOIAS_003361"
     CDS             321..1514
                     /gene="nifV"
                     /locus_tag="ACOIAS_003361"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HGZ2703032.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1539..3464
                     /locus_tag="ACOIAS_003362"
     CDS             1539..3464
                     /locus_tag="ACOIAS_003362"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HGZ2703033.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3486..4481
                     /locus_tag="ACOIAS_003363"
     CDS             3486..4481
                     /locus_tag="ACOIAS_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HGZ2703034.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4831..5280
                     /locus_tag="ACOIAS_003364"
     CDS             4831..5280
                     /locus_tag="ACOIAS_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGZ2703035.1"
                     /translation="MIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKGQFTFLTRI
                     CENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLTPTDKSLEV
                     YELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5314..5808
                     /locus_tag="ACOIAS_003365"
     CDS             5314..5808
                     /locus_tag="ACOIAS_003365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2703036.1"
                     /translation="MIRKANMNDLESIMQIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5905..6426
                     /locus_tag="ACOIAS_003366"
     CDS             5905..6426
                     /locus_tag="ACOIAS_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2703037.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6615..6926
                     /locus_tag="ACOIAS_003367"
     CDS             6615..6926
                     /locus_tag="ACOIAS_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGZ2703038.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6954..7640
                     /locus_tag="ACOIAS_003368"
     CDS             6954..7640
                     /locus_tag="ACOIAS_003368"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HGZ2703039.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3085 a          758 c         1493 g         2358 t
ORIGIN      
        1 ttaaattaaa ttttagtagg aattttatag gtttattttt aaaggttttt tgaaaataaa
       61 tctatttaaa aattaaatta aaatgcaaga aaaacataat aactttcatt tttaagaagt
      121 tttcacaaaa aagaaaaaat atagtgcaat aaatgataca aagtaatata atagattaca
      181 agatatgaaa agctaaaaaa ataaattgca aatgaaatta aaatgaataa aaaataaaaa
      241 aataatcata agttagaggt atcaatgaaa aataagactt aatgttttaa aattaaaagt
      301 atactaaggg gggcattaat atgtgcgtta tatcaaaaga tagagcaaag gaaataaaaa
      361 tagtagatac tactttaaga gatggagagc aaacagctgg ggttgtattt gcaaatagag
      421 aaaaaataat gattgctgaa atgttaagtg acctaggtgt tgaccaaatt gaagttggta
      481 tacctactat gggtggagat gaaaaaaatg taataaagca catatgtagt agaaatttaa
      541 aatcagatat aatggcttgg aatagagcag taataaaaga tgttgaagaa tctatttctt
      601 gtggtgttga tgcagtagct atatcaatat cagtatctga cattcatata gaaaataaat
      661 tgagaacttc tagaggctgg gttttagaaa atatggctaa aacagttgaa tttgcaaaga
      721 aaaatggatt atatgtttca gtaaatggag aagatgcttc aagagcagat atagacttct
      781 taactgaatt tataaatgtt ggaaaacaag caggggcaga tagatttaga tactgtgata
      841 cagtaggagt tatgaatcct ttttctataa agaatgcaat agaaactctt tatgaaagaa
      901 ctaattttga tatagaaatg catacacata atgactttgg gatggcaact gcaaatgcac
      961 ttgcaggaat agcagcagga gcaaattatg ttggtgttac agtaaatgga ttaggtgaaa
     1021 gagcaggaaa tgcagcatta gaagaagttt taatggcact taagtgtgtt tataaatgtg
     1081 atttaaataa tatagatact agaaaattta gaggaatatg tgagtatgta gcacaggcat
     1141 caggaagaat acttcctaca tggaaaccag tagttgggga taatatgttt atacatgaat
     1201 ctggtataca tgcagatgga gctttaaaag acccacataa ctatgaacca tttgacccaa
     1261 gtgaagtaaa tcttgaaaga aaaatagtaa taggtaaaca ttcaggaaga gctgcagttg
     1321 taaataagtt gagtgaatat gaaatgtata tttctccaga aaacgctacc aagctattaa
     1381 atgctataag agctacatct ataagattaa aaagaagttt aatggataaa gaaattcttc
     1441 aattatattg tgatatacta gcacatgaga aaggcacaac agaagaagaa gcagttagag
     1501 gttcatatat ttaagatata attgtttagg aggaaaatat gggagataat atagtttata
     1561 aaataattaa aaaacatata gtagatggag aagctgttgc tggaagttca attggtataa
     1621 aaatagacca aactttaaca caagattcta ctggaacaat gacatatctt caactagaag
     1681 ctatgggaat agataaagta aagactaaaa gaagtgtggc atttgtagac cataatatgt
     1741 tacaacaagg atttgaaaat gcagacgacc ataagtatat acaaacagtg gcagataaat
     1801 atggagttta tttctctaag ccaggaaatg gtatttgtca tcaagtattt ttagaaagat
     1861 tttcaacacc aggagataca cttttaggtt ctgatagcca cacacctact gctggaggtg
     1921 tgggcatgat ggctattgga gctggtggat tagatgttgc tttagccatg gcaggaggag
     1981 catactatat aaaagcacca aaagtctgta aggtgaatct agttggaaaa ttaaataata
     2041 tggtatcatc aaaagatata atattagaag tgttaagaaa acaaactgta aaaggtggag
     2101 taggtaaagt ttatgaatat ggtggagaag gtgtcaaaag cctatctgtt ccacaaagag
     2161 ctacaataac aaatatgggg gctgaacttg gagcaactac ttcaatcttc ccaagtgatg
     2221 aaaaaacact agaatttttt aagagtcaag gaagagaaga ggcatggata gagctaaaac
     2281 cagatgcaga tgcagtttat gatgaagaga ttactataaa cttggatgaa ttaaaaccat
     2341 tagctgcaaa acctcatagc ccagacaatg tagatgaagt tgaaaatata ggcaagatta
     2401 aaatagacca agtggcaata ggaagttgta caaattcttc ttatgaagac ttaatgaaag
     2461 tagcacaaat cctgaaagga aataaagtac ataaggatgt gagtttggta atagcaccag
     2521 gttcaagaca agttatggag atgattgcta gaaatggggc tttggcagat ataataagtg
     2581 ctggagctag aatactagaa aattcttgtg gtccatgtat tggtatgggt caatctccag
     2641 gtactgactc tgtgtcttta agaactttca atagaaattt ctatggtaga agtgggactt
     2701 tgtcagcaca agtatattta gtaagtccag aagtggcagc agtaagtgca atcaagggag
     2761 tgcttacaga cccaagagaa tttgatatta aatttacaaa tttagatgta aatgaatttt
     2821 tgatagatga tagcatgata attaaacctg ctgatgtagg aagtgatgta gaagtagtta
     2881 gaggacctaa tataaaacct ttccctctta atacagagct aagtcagtct ataggtggaa
     2941 aagttatatt aaagacagaa gataatataa ctactgacca tataatgcca tcaaatgcta
     3001 agttacttcc ttttagaagt aatataccgt atcttgcaaa ctattgtttc aatactgtag
     3061 atacagaatt tcctcagaga gcaaaagaca ataatggtgg atttatagtt ggtggagata
     3121 attatggtca aggttcaagt agagaacatg cagctttagc accattatat ctaggtgtta
     3181 aaggggtaat agttaagagc tttgcaagaa tccacaaggc taacttgata aatagtggta
     3241 taattccaat ggagttttgt gatgagaaag attatgaaaa tatatctctt ttagataatc
     3301 tggagatacc aaatatttta gacaatctag gtagtggaat tttagaagtt aagaatacaa
     3361 ctaaaggaac ttcttttaag gtcaaagtag aattgtcagc aaaagaagtt gatgtattaa
     3421 aagctggtgg aaaattaaac tacactaaaa atcaagctaa ttaatatgaa aagagggaat
     3481 attgaatgta taaagtgaca cttataccag gagatggaat aggaccagaa gttgcgaaag
     3541 ctatgaaaaa ggtagtagaa gctactggag tagaaataga atgggaagaa gttaatgcag
     3601 gagaggcagt tattgaagag tatggtactc cacttcctga atatattata gatagtataa
     3661 aaaagaacaa aatagctata aaaggcccaa ttactactcc tgtaggtaaa ggatttagga
     3721 gtgtaaatgt aactttaaga caggcattag atttgtatgt caatttaaga cctattaaaa
     3781 gttttaaggg gataaaatca agatatgaag atgttgattt agtggttgta agagaaaata
     3841 cagaagattt atatgctgga atagaacata aaataggtga ttatgcagct gaaagtataa
     3901 agataataac aagaagtgca agtgaaagaa tcgttgattt tgcatgtaac tatgttaaag
     3961 ataataagag aaaaaaagtt acagctatac ataaggcaaa tataatgaaa atgtctgatg
     4021 gattgttttt agatgtgttt agagaagttg catcaaaaca tggtgtagaa tatgatgatt
     4081 tgattgtaga tgcagcagct atgaacttag ttttaaatcc agaaaattat gatgtaatgg
     4141 taatgccaaa tctttatgga gatatactat cagatttagg tgctgggctt gttggaggtt
     4201 taggtataat tccaagtgca aatattggta aagattgtgc gatatttgaa gctgttcatg
     4261 gaagtgctcc acaaatagca ggtcaaaata aggctaatcc aacagcactt attcaatcat
     4321 cagtaatgat gcttagatac ttaggtgaat atgaaaatgc acaaaaaata gaaacagctt
     4381 tagaaaaagt atttcttgaa ggaagtaaat tgactgtaga tttgggtggc tcagcttcta
     4441 ctacagagtt tgcggatgag gtgtgtaagt atattgtata gattttattt agggaaaaag
     4501 taaaatatag attgaaggaa agaggctttc tcaaaataga gataagcctt ttttattgga
     4561 atgataaaat atatttgatt ttatacaaaa gagagttcct cataaacgaa aaagttaatt
     4621 ttgggatatc cattttaatt ttaaaattaa ttgattaata tgcaaagaaa gattaaataa
     4681 atttattatt tttaattgaa ctttcaacca aaatatggta aaatttaaaa aagttgaact
     4741 ttcaactaaa aatgaatata ttgattatgt gaaattttat aaaacagaaa ctatttgact
     4801 ttagccatag tagtttcagt ggaggtgtgt ttgattaaga ctttagatag taatatatta
     4861 agagaagtag gtactttatc tagagcagta aattcaataa atgatattaa gtacaaggaa
     4921 ttaaagttac aaaaaggaca atttacattt ttgacaagaa tctgtgaaaa tcctggtata
     4981 aatcttgtgg aattatcaaa tatgttaaag gtagacaagg ctacaacaac caaggctata
     5041 caaaaattaa taaaggcagg atatgtagat aaaaagcagg ataagtttga taaaagagga
     5101 tataatttaa ctccaactga taaatctttg gaagtttatg agttgataat tgaagaagag
     5161 aatagaagta tagagatatg ttttgataat tttacagatg aagaaaaaca agtggtaact
     5221 aagttattag aaaaaatgag caaaaacgta gaaaatgaat ggtttaaagt aaagagatag
     5281 taaataaata cattaactta aggagatatt tttatgatta gaaaagcgaa tatgaatgat
     5341 ttagaaagca ttatgcaaat tataaaatct acagtggaag aaatgaagac ttataataat
     5401 acacaatggg atgaaaacta tccattagaa aaagactttg tttcagatat taagaaacaa
     5461 gatttatata tttatgaagt ggatggagaa gtagcagggt ttatatgtct taattatgaa
     5521 gagccagaag aatatttagg cttaaattgg tcatctaata aaaaagcaat ggtaatacat
     5581 agaatggctg taaatccaaa ctttagaaag acaggaatag catctaagtt agttgatttt
     5641 gctgaaaaat tagctgtaga aaacaatgta tcatacttaa agagcgatac atattcaata
     5701 aactcaaaaa tgaatttatt acttacaaag tgtggtttta taaaaatcgg agaaatgagt
     5761 tttttaggaa aggagaaatc tttctactgt tatgataaaa tattataaag agcatttata
     5821 atacaaggga taaataatag attttcagca ttagtaaggg cataatataa cctagtgtat
     5881 gctattatga aagggatgat ttatatgatt ggatttctaa agaaaagaag aagtattaga
     5941 aaatacaagg atgttgaagt agaaaaagaa aaattagata aaatattaaa agctgctctt
     6001 ttagctccat catcaaaggg gcttagaaca tgggaattta tagtggtaga tgataaagaa
     6061 aagctaatta atttatctca atgtagaact aaaggtggtg gatttttctt aaaaaatgca
     6121 cctttagcta ttgtaattat tgcagataaa gaaaaaaatg atgtttggat agaggatgca
     6181 agtatagctg cttcatacat acaacttcaa gctcatgaac taggtttagg ttcttgttgg
     6241 atacaagtaa gaaatagaat gcatgatgat aatatagaag ctgataagta cattagagaa
     6301 gaattaaaag tacctagcaa gtacagtgta gaatgtataa tttctatagg atatcctgat
     6361 gaagaaaaga aagcatacgg tgactctgat ttagactata aaaaggttca ttttaacaac
     6421 ttttagtata taatatgttg tattaagaca ttatattttt ttctattgtt aatatatact
     6481 taaaattgta aaaatatatc tgaattttac taacttatct aggggttatg tacaaggatt
     6541 ttaaagataa atatagaaat taatgtttat aatcgataca taacttgagt aacaaaattt
     6601 aaggaggtta aagtatgggt atatttaaaa caaaaatgga tgaggactgg aaagtaaact
     6661 acattaaaga gtttaatgaa atgagagatt cttatgagag taaacttcaa aaaaaacagt
     6721 ttgaggtaga tagtttgaaa tcagaattgg atagactaag aagctataaa aatagcctta
     6781 agccaaaaga aaaacaaatt actgatgatg atataaataa tataaagagt ttaagaagag
     6841 atggcttaag ctataaagaa atttcaaatc aaacaagttg gagcaaagca actgtaagta
     6901 gggtattaaa tggactttac gattagcata tacatggggt gatttaaatt gggatgaagt
     6961 atagttacaa aaaatgcata atagattcta ttttattgat gtttatagtt caaatattaa
     7021 ggatgatttt gaattatgta cttctaagtc aatttgaatt tactcttgaa aattttaata
     7081 ttataaactt aatttctttt acattagttg gtttatcgtt aattttattt ttaaaagata
     7141 attctttgta taacaaggtt agaaatagaa aaatcacaga agcttttgaa gaaaataaaa
     7201 acaatatact tattgaaaag tgtaagctga tactttttgt tgtggtttta tctttagcaa
     7261 taattgtaac ttattgtact aagggatatg tactttttaa tgtaactatg atgactttgt
     7321 ctgtattgat agtcccaata tttgaagagt tattttttag agaatatata tggaactatc
     7381 taagtaattt tataaagtca aagagtaaaa ttatctgtat aacatctata ctatctggaa
     7441 tttataatat aggatacata gatgttataa gaaattatgt aatattgtat aacaattcaa
     7501 gctacacctt tgaagttatt atttcaaaaa ttatgatagg tactgttttt ggtattgtac
     7561 ttggattagt taagtatagg tttagagatg tagggttttg tatattattg aggagcttat
     7621 ttgctatatt tattaggtag atagtataat cggtttataa ggttgtttta aatttaattt
     7681 taaatcaacc ttat
//
LOCUS       DBGJFU010000160         7641 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.163, whole genome shotgun sequence.
ACCESSION   DBGJFU010000160 DBGJFU010000000
VERSION     DBGJFU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.163"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..94
                     /gene="rrf"
                     /locus_tag="ACOIAS_003369"
     rRNA            <1..94
                     /gene="rrf"
                     /locus_tag="ACOIAS_003369"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            220..729
                     /locus_tag="ACOIAS_003370"
     CDS             220..729
                     /locus_tag="ACOIAS_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HGZ2703040.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            741..1622
                     /locus_tag="ACOIAS_003371"
     CDS             741..1622
                     /locus_tag="ACOIAS_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGZ2703041.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1750..1955
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2081..3352
                     /gene="serS"
                     /locus_tag="ACOIAS_003372"
     CDS             2081..3352
                     /gene="serS"
                     /locus_tag="ACOIAS_003372"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGZ2703042.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3673..4128
                     /locus_tag="ACOIAS_003373"
     CDS             3673..4128
                     /locus_tag="ACOIAS_003373"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HGZ2703043.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4129..4220
                     /locus_tag="ACOIAS_003374"
     tRNA            4129..4220
                     /locus_tag="ACOIAS_003374"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4163..4165,aa:Ser,seq:gga)
     gene            4258..4522
                     /gene="ffs"
                     /locus_tag="ACOIAS_003375"
     ncRNA           4258..4522
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACOIAS_003375"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4599..6236
                     /gene="dnaX"
                     /locus_tag="ACOIAS_003376"
     CDS             4599..6236
                     /gene="dnaX"
                     /locus_tag="ACOIAS_003376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGZ2703044.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            6295..6645
                     /locus_tag="ACOIAS_003377"
     CDS             6295..6645
                     /locus_tag="ACOIAS_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGZ2703045.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6658..7257
                     /gene="recR"
                     /locus_tag="ACOIAS_003378"
     CDS             6658..7257
                     /gene="recR"
                     /locus_tag="ACOIAS_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGZ2703046.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            7398..7619
                     /locus_tag="ACOIAS_003379"
     CDS             7398..7619
                     /locus_tag="ACOIAS_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703047.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
BASE COUNT         3096 a          778 c         1421 g         2346 t
ORIGIN      
        1 tacacctgtt cccattccga acacagaagt taagctctct agcgctgatg gtacttggtg
       61 ggaaactgcc tgggagagta ggacgtagcc acgtagtctt ttttatttat tataaaagtt
      121 aagattaaat agaatataaa gtcaatattg atttataata agataaaata taaaattata
      181 tagtataggt aaagttttta tttggaaaga aggtgtaata tgaattctaa tgaaagaaga
      241 agtaaattaa tagatatttt aaaagaatct aagcatcctg taaaaggtgg aacactagct
      301 gaactattaa atgtaagtag gcaagtaata gttcaagata tagcccttat aagagctaga
      361 ggatttgaaa taatagctac acctcaagga tatataatat ataatcaacc gtattttaca
      421 aaacaaataa agtgtaaaaa tcataaggat tctaaagaaa tttatgatga attaaagata
      481 attgtagatt taggtggtat tataaaagat gttattgtaa atcatccaac atatggtcag
      541 ataactgcag aacttaatat ttattctaaa atggatatag acaactttat gaagaaagtt
      601 gagacaaatg aatttaaaca attatcggtt ctgacagaat gtagccatat tcatacaatt
      661 gaggcaataa aagaagaaac tatagaagct atagtaaaag agttaaagga caacggaata
      721 ttatcataag gaggagttga atgaattttt tatcattggc taagactgcc tcaacaaata
      781 gcaatataac taagagaact ttagaaaata tgctcgataa ttttagtgat aatataccaa
      841 atattatata tgcaataatt gtatttgtga taggtattta tatatctaaa atagtaagaa
      901 ggatggtttc aaagtttcta atcaaatatg gaatgagtaa aggtgtaaat aattttattg
      961 tatatggaat atatatatct atgttatcta ttatatcatt gataagctta ggtattatag
     1021 ggatacaaac tacatcagtt gtagctgtgt taggagctgc tggatttagt ataggtttag
     1081 cttttaaaga aatattatca aatttaggct ctggtatgat tattttattt tttaagccat
     1141 ttaatatagg tgattatatt caaggttcag gtgtagaagg tacagtttct gacatacaaa
     1201 tatttagcac tgttcttaag acgccagata ataaaactat aattatacct aattttcaac
     1261 tgacaagtaa taatataatt aattacacac atcaaaacaa aagaagaata gatttttcat
     1321 ataatatttc atatgatagt gatatagatg tagttaagtc tatattaaat gaaattttta
     1381 caaacgaaaa aagaatatta aatgatccaa agcctataat aggtctaaac tctataggaa
     1441 ataacaccat gcaaatagtt gctagacctt gggtaaaaac tgatgattac tgggatgtat
     1501 actttgatgt aatggaaaag gtaaaaaata aatttgatga gaatgatata aaagttccat
     1561 ttgttccttc ttcattatta tttaataata caggtaatct aaatacaatg gataaaagat
     1621 aaggtcttaa ttaaatagaa aaaatgaatt taatataaaa aattaaagaa aattctaaaa
     1681 aaatttgaat aatagtattg aaagatgtta atatatataa tataatgatt atcaaataaa
     1741 acaattagtt aaaaagctgt gaaggaaaat agtaaaaaac agattgtatt cagagaatga
     1801 gtggatggtg aaaactcatt acaatggttt ttgaatctac tctagagctt ctagttaaag
     1861 ctagacgttt acctacgtta taggtctgag aggattatac attttagtat agtcaattag
     1921 ggtggcaacg cggataaaag attttcgtcc cttttatagg gtaaacggag gtcttttttt
     1981 atttttaaaa tttgtttaaa ctaagagttt acaagtataa tgtttgtaaa ttaagttgga
     2041 attataactt atattatcaa aattattagg aggaattaat atgttagata ttaaaagaat
     2101 aagagaaaat ctagatgata tcaaaaaagc tatggaaaga agaggtgaaa gagaatttga
     2161 ccttgatgct gtagtggaat tagataataa aagaagagaa atacttcaag aagttgaagt
     2221 tatgaaaaat gagttaaatg taaattctaa aaaaatacct caattaataa aagaaggaaa
     2281 agatgtaaca gaagaaaagg caagattgaa agagttatca gataagataa aaggtattga
     2341 tgagaaagta aaagaagtag aagcaaaaat ggaatataca ttaatgagaa taccaaatgt
     2401 tcctcatcca gaggtaccac aaggtgaaac agatgaagac aatgttcaaa taagaacttg
     2461 gggagaacct actaaatttg attttgagca taaagcgcat tgggatatag gtacaggtct
     2521 aggtatttta gattttgaaa gagcagggaa aataactggg tcaaggttta ctttatatag
     2581 gggattagga gctagattag aaagatcttt aatgaatttc tttttaaata ctcatacagc
     2641 taagcatggc tatacggaag tcttacctcc atttatggcc aatagaaata gttttatagg
     2701 gactggtcaa cttcctaaat ttgaagaaga tatgtttaaa atagaaggat tagagtattt
     2761 cttaatacca acagctgaag ttcctgttac taatatacat gctaatgaaa tattagatgt
     2821 tgcagagctt ccaattaaat actgtgcata tactccttgt tttagatctg aagcaggttc
     2881 agcaggaaga gatacaagag gtttagttag acaacatcaa tttaataaag ttgaattagt
     2941 taaatttgtt aaaccagaag attcttataa tgaattagaa tcattaactc atgatgctga
     3001 aactatgtta caaatgttag gtcttccata tagagttgta aaaatatgta caggagattt
     3061 gggatttaca gctgcattta aatatgattt agaagtttgg atgccaagct acaatagata
     3121 tgtagaaata tcttcttgtt caaattttga agatttccaa gcaagaagag caggaataag
     3181 atttaaaagg gataaaaaat caaaagcaga atatgttcat acattaaatg gttctggatt
     3241 agcgatagga agatgtttgg ctgctatatt agaaaattat cagcaggcag atggttctgt
     3301 agtagttcca gaagaattaa gaccttatat gggtgttgat gttataaaat aaatttatat
     3361 taattgtata tgtagagtat gctttgatga agagaatata ttatgagtaa acaataaata
     3421 aaaaaactat gaaattatta taaatattaa tttcatagtt tttttatgtt aatttacaca
     3481 gcagaatttt catcataaaa cactaaatat ttttataaaa cactcttgta tgaatatcta
     3541 aaaattattt tagaactaat ttaaaaaaaa tatagctata tatagtaata attaaataaa
     3601 caaaataaaa ttaagttgaa ttataaaatg aattatgata agataagttt gttataagtt
     3661 ttgggaggaa ttatggaaag ttcattttat atgaaagaag cattaaaaga agcatataaa
     3721 gcatataata aaaaagaaac accaataggt gctattatag ttaaagataa tcaaataata
     3781 gctagagctc ataaccttac agaaacgtta aaagactcta cggcacatgc agaaatttta
     3841 gcgataaaac aagcatcaga aaaattaggt ggttggaggt tgactgattg tgatttatat
     3901 gttacaatgg aaccttgcat aatgtgtagt ggcgcaatag ttaattctag aataaaaaag
     3961 cttataatag ggacaagaca tgtaaaaaat tcatatatag aaaaacagca tgagtttaaa
     4021 ttagattatt ttaataataa taatgtaaaa gtagcttttg atgttttgca agaagaatgc
     4081 tcgatcatat tgcaagaatt ttttaaggca ttgagaaaaa gagattaagg agagatggtc
     4141 gagtggtcga aggcgctcgc ctggaaagcg agtatatggg taaaactgta tcgtgggttc
     4201 aaatcccact ctctccgcca gaaacaatga aagtaattaa atatggttgt aattttgctg
     4261 tactagatgg ggaggtagcg gtgccctgta acctgcaatc cgctatagca ggattgaatt
     4321 ccatctaaag gcttattttc tgtagggccg ccctaaataa gtggtgttga tgcttaggtc
     4381 ctgcgcaatg aaagctcatg aaccccgtca gatccggaag gaagcagcgg taagtgatca
     4441 cttttgtgtg ccgtgggggt gcctaagttg agctaactgt tttggtaacg cctatggaat
     4501 taagtcgata gatggtgtac agcatttaat atataaaaga ctaatccctt agataatgag
     4561 ggattttttt attaattttg ctaaagagag gtaaaaagat gcataaagca ttatatagag
     4621 catatagacc acaaaaattt gaagatgtta ttggtcaaga tcatattatt aagactttaa
     4681 agaatcaaat atatagtgat aatataggac atgcttattt gttttgtgga acaagaggta
     4741 ctggtaaaac atctactgca aagatttttt caagagcagt aaactgtttg aataaaataa
     4801 atgaagaacc gtgtaatgag tgtgagatat gcgaatctgt attaaaagat aatacaatgg
     4861 atgttgtaga aatagatgca gcttcaaata atagtgtaga tgatataaga gagcttagag
     4921 aaagtgtgaa atattctcca gccaatgcta aatataaagt atatataata gatgaagttc
     4981 atatgttgtc tcaaggagct tttaatgcat tattaaagac cttagaggaa cctccatcat
     5041 atgtaatttt tatattagca actacagagc ctcacaaaat acctgcaacc attttatcaa
     5101 gatgccaaag atacgatttt aaaagagtta cagttaaaga tatgaccctt agaatgaaaa
     5161 agatttgtga agatgaagga attgatatag atgataaagc tcttaattta atagctagaa
     5221 attctcaagg agctcttaga gatgcactta gtatattaga ccaatgtatg tcttttggag
     5281 aaagtaagat agattataaa gatgttgttg aattaatggg aagcgtaaat atagaacaat
     5341 tatttgaatt gtctcaatgt attgtagagc aggacactaa aaagtctctt gagatattaa
     5401 atgaatttgt cttatggggc aaagatataa gaaatcttat aaatgactta attgatcatt
     5461 ttagaaattt aatggtatgt aaggtttcat cagaattgga tgaaataatt tcattaccag
     5521 aagaaactat agaacaactt aaaattcagt ctaaaaatat agatataaat gatttaataa
     5581 ggattttaaa tatattatca ataactcagg atgatataaa gtcatcttca aatccaaggg
     5641 ttttagtgga aataaccata atgaaaattg ctcaaccaat gtttgatgaa agtaaagaag
     5701 ctcttataaa aagagttgag aacttagaaa aaatgattga actggggaac tttaagtctg
     5761 aaaaaattgg gaataataaa gaaaaagagt atgaggtcga tagagaaata gatgtaaaac
     5821 aagaaaatgt agtttatgaa gatgtaaaaa atgaggatgt tatacttata gagtcatctt
     5881 ggaagaatat acttaaacaa attaaaaaag ataaaaaaat gccaatatac gcactcttaa
     5941 gtgaagtaaa aagttttaat gtatattcta atatgttgta tgttatattt gatgataaat
     6001 ttgattttgc aaaaactaga ctgagctctc aagatacaat aaattattta gaaaaaacaa
     6061 taagagatgt tttaaataga agttttaatg taaaaatagt actgacatca gaagttaaag
     6121 atataaatct tgaagttaaa gagaaaaagg acattggtga ggaaatatta aagaacatag
     6181 tgagtgaaga gatattagaa ataaaagata gtattgatga aaatgagagt aaataataaa
     6241 atactattat gatataataa taaaatgaaa ataagtttag gaggaatagt gtttatggct
     6301 aaaaaaggtt ttggaggcgg aatgatgcct ggtggtggaa atatgaacaa cttgttaaag
     6361 caggctcaaa aaatgcaaga aaatatgcaa aaagctcaac aagagttaga aagtaaagaa
     6421 gtagaagctt cagttggtgg aggagcagtt actgtaaaag ttaatggtaa aaaagaagtt
     6481 atagatataa ctatcaagcc agaagtagtt gatccagatg atatagaaat gctacaagat
     6541 ttagttttaa gtgcagtaaa tcaagcattg agaaatattg atgatataca agcaagtcaa
     6601 atgagtaagg taactggagg tatgaatata cctgggttat tctagtaggt ggtatttatg
     6661 caagtttata caggtcccat aacgagactt atagaggaat tttcaaagct tcctggagta
     6721 ggaagaaaga ctgctcaaag attggctttt catattatta atatgaatac taatgatgtg
     6781 gaagctttat ctaaagcgat tatagatgca aaaagagaaa taagatattg ctcaatttgt
     6841 tgtaatataa cagatacaga cccatgtagc atgtgttcaa ataaaagtag agattcaagt
     6901 gttatatgtg ttgtggaaga tccaagagat gtagcagcta tggagagaac gagagagttt
     6961 aaaggacagt atcatgtatt aaatggagta atttctccaa tggatggaat tggaccagat
     7021 atgttaaaaa taaaggaact tatccaaaga cttggcaatc aggaagtcaa agaaataata
     7081 atggcaacaa atcctactat tgaaggtgaa gctacagcca tgtatattgc taggttagta
     7141 aaaccaatgg gaatcaaagt cactcggata gctcatggtc ttcctgttgg aggcgattta
     7201 gagtatgcag atgaggtcac aatatcaaaa gcactagaag gcagaagaga aatataaaaa
     7261 tcgaggttaa tacctcgatt tttgtagttt tttgagttaa attcaaatta attttgattt
     7321 agatttattg aataaatttc ttctgtattg tataatttgt tgtataaatc atttaaaaat
     7381 tttgataggg gtgcaacatg aaaccaatta tgaacttaga agaacataat gcaatgaaag
     7441 aaaaaagagt taaaaaaatg aaatcaaaag ataaacctaa atatgatgat tttgaggata
     7501 taagtttttc taataagaaa aaaactaaaa aaaatgtacg aaataaaaat gctgaattta
     7561 gaaataatta taaaaattat cagtatgatt atgaagaaga cttttatgag ccaatttaat
     7621 attaaataat tagaaaataa a
//
LOCUS       DBGJFU010000161         7599 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.164, whole genome shotgun sequence.
ACCESSION   DBGJFU010000161 DBGJFU010000000
VERSION     DBGJFU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.164"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            163..504
                     /locus_tag="ACOIAS_003380"
     CDS             163..504
                     /locus_tag="ACOIAS_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGZ2703048.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            655..1068
                     /locus_tag="ACOIAS_003381"
     CDS             655..1068
                     /locus_tag="ACOIAS_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HGZ2703049.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDHIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1270..1911
                     /locus_tag="ACOIAS_003382"
     CDS             1270..1911
                     /locus_tag="ACOIAS_003382"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGZ2703050.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1935..3188
                     /locus_tag="ACOIAS_003383"
     CDS             1935..3188
                     /locus_tag="ACOIAS_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGZ2703051.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3234..3884
                     /gene="cmk"
                     /locus_tag="ACOIAS_003384"
     CDS             3234..3884
                     /gene="cmk"
                     /locus_tag="ACOIAS_003384"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGZ2703052.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3935..4531
                     /locus_tag="ACOIAS_003385"
     CDS             3935..4531
                     /locus_tag="ACOIAS_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGZ2703053.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4544..5383
                     /locus_tag="ACOIAS_003386"
     CDS             4544..5383
                     /locus_tag="ACOIAS_003386"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGZ2703054.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKKVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5628..6782
                     /locus_tag="ACOIAS_003387"
     CDS             5628..6782
                     /locus_tag="ACOIAS_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HGZ2703055.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7088..7267
                     /locus_tag="ACOIAS_003388"
     CDS             7088..7267
                     /locus_tag="ACOIAS_003388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703056.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEID"
BASE COUNT         3155 a          730 c         1328 g         2386 t
ORIGIN      
        1 tttttaactt taaaaattaa tgttaaaaaa taaacttata cattaatatt aataataatt
       61 atttaaatat cttaagtaaa tcagtcttac atatatttcc gatagattat attgataaaa
      121 aacactaaaa ttaaattaat aaaagtactt ggagggatgg aaatgaaaga ggaaaatttt
      181 attaatgttg aatacgagga taaacgtttt tcaattaata gtaaagaagt tttgtacata
      241 gaaaagcaag agtatgaagt aaagatttat actgaaagtg gtaattatga agttggagaa
      301 aatgctcctt gtataagaaa actattgaaa gaagaaaatt ttttcaaatg cagtaatagt
      361 tatattgtaa atctagacaa gtgtgaattt tatctcagca ataattgtat ttttgtgaat
      421 aataatttta taagaatttt aggttcagaa agaaaaaaga aattaaaaaa gaaattagaa
      481 aaattatatg attatgaaga ttaatattgg gaggctgtat tttatacagc ctctttattt
      541 attgattgta ttattaataa attttaaatt atgataaaat ataaaacaag taacttattt
      601 cagaataaaa tactttttaa aagaaaggga cgagatatgg gagattataa taaaatgctt
      661 gaattaaaga ataacttagg tatcagtgaa agaaagttaa aatatccttg tatctataaa
      721 catttcaaag ggaaatatta tgctacaatg ggtcttagca aggctattga tcatatagaa
      781 aacatatgtg aaatatatgg taaagaaaat ttaatccaaa acagaaataa atataaatta
      841 gttattagac atactgaaag agaagaagat atctatgttt atagggattt agatggaaac
      901 ttttatcata aaaaagaaga agatactaat gatttagttt tatacaagac cttatatgat
      961 gatacaggaa tatttgcaag acctttggat atgtttttag aaaaagtaga tacagataag
     1021 tatgtcaatt caattcaaga atacagattt gaagaagtat ataaataata taagagaaat
     1081 atgtttataa atttagaaaa attctttaac agtacaataa tttgcagaaa tacaaatgag
     1141 atttattgta aataatatgt tagattagat tataaaatag aaacaaagta aagtattata
     1201 tgatataatt aagttttgag aagtgataat ttatatttaa taaaaaatag gcatatatta
     1261 ggaggacata tgggaaatac tttttatata gtaagacatg gacaaacgga ttggaatata
     1321 ttgggaaaaa cacaaggtca tggtaattct gaccttacgc ctcaaggtat agaacaggca
     1381 aaagaacttt ctgaagatat tggcaagtat tcaattgatt atatttttag tagcgacctt
     1441 gggagagcaa tgcaaacagc tcaaatttta ggagataagc tcaatattga agttcaaaaa
     1501 actgaggcac ttagagaaat gggattcgga gtttgggaag gccttttaat aaaagaaatt
     1561 caaaaagatt attcagatat atatgcaaca tggagaaatg aaccacactt agtaaatata
     1621 ccagaaggcg aaactcttaa gattataaag gaaagagtag atgcttttat aaaggaacta
     1681 aatgaaaaat atgataataa aaatataatt cttgttacac attcaattac attgagggtt
     1741 atgttgttgt catttttaga atcaggtatg gaaaatatat atagaattaa gcaagataat
     1801 actgctttaa atatagtaga atttaaagat tatgggcctg ttatagtaaa aatgaatgat
     1861 acaagtcata taaaaaatca tgtaaagata aataattctg cactggagta ggaatcaatt
     1921 ataaggagaa atttatgagt aaagttatag tagttggtgg aggagcttct ggaatgatgg
     1981 cagctttgtc agcttcaaaa aacaataatg aagtaatatt agttgagaga aatggagaat
     2041 taggaagaaa acttagggca actggtggcg gtagatgcaa ttttaccaat aatagagaga
     2101 tagaagattt ttttgataaa gttgtcagta ataaaaaatt tctctatagt agtttctata
     2161 catttacaaa taaagacctt atttcgtatt ttgaaagtag aaatttggaa tataagattg
     2221 aagaagaaaa tgaccacaaa gtatacacta aaaatgataa atctatagaa gtaatagaag
     2281 tattaaataa agatttactc aatcacaatg taaagataat gtataataag aaagttatag
     2341 atattataac tgaagaaatt gctctaaaag atgattctaa taaggacaag tcaaagtatc
     2401 taataaaagg gattatttta gataatggag ataaaatact aggagacaag gttattatat
     2461 caactggagg agtgagttat tcaaagacgg gttcagatgg ttcaatgtat aagattctta
     2521 aaaagcatgg acatacatta aacaaactat atcctgcttt agttccactt acaatagagg
     2581 aaaaatggat taaagattta caaggaatat ccatgaagaa tgtggaaata tcttgtaaga
     2641 ttaagaaaag gaaaatatca aaatctggag acatgctatt tgcccatttt gggataacag
     2701 gaccttgtgt tcttataatg tcttcttata ttaacaagat tattgaaaag gaaaaggtag
     2761 aattaaatat agatttctta ccaaatttaa gtactgatga aatctctagt ataatcagag
     2821 catttccaaa taaaaatgtt ctaaataatc ttaagcaaat actacctcaa aatttcttaa
     2881 aagagatttt ttctctatta tctttagttg ataaaaaggc aagtgatttg tctaaggcag
     2941 atgaaattag aattattgag tatataaaaa atatgaagct aacttgtaat gggactacag
     3001 gtataaacac tggtatggtt acttctggtg gtatttcagt taaagaaatt aattcttcta
     3061 ctatggaatc taaattagtt aagaacttat tttttacagg tgaagtaatt gatatagatg
     3121 cagaaactgg tggatataat ttacaaatag cattttcaac tggatatctt gcgggtataa
     3181 gtgtataaat ataatttttt aataattaaa tatgaataaa tagaggtgta tatatgggta
     3241 atttagttat agctgtagat ggtccagcag gagctggaaa aagtacaata gccaagatag
     3301 tagcaaaaaa gctaaatata aattatatag atacaggtgc tatgtacaga gcagttactt
     3361 ataagtgttt aaaaagtggt attgatgtca ataatgaaaa agaagttatt caaatagctg
     3421 aaaattcgga tatagatttt aaagataaca atatatattt ggacaaagaa gttataaatg
     3481 aagaaataag aacaatagaa gtaagcaata atgtttctaa tgtagcaaaa ataaaagaag
     3541 taagacagtt gatggttgaa gttcaacgaa aaataggaat gaaaaattca gtaatactag
     3601 atggtagaga tataggttca tatgttttcc cagatgcaga ttacaaattc tttttagtag
     3661 ctactccaga agaaagagga aatagacgct ataaagaact ttgtaataaa ggatataata
     3721 ctactttaga agaagtaatt gaggatataa taagacgaga tgaaattgat tcaaatagag
     3781 aatttgcacc attggtaaaa gctaatgatg cactagaaat agatactaca ggaaaaacta
     3841 tagaagaagt tgtagaagaa gttgtatcta aaataaatct ttagtttttt atgcatcata
     3901 atgaaaataa atgtagtgtt gaagggagat aaacgtgaat ttttatagat ttgtaataaa
     3961 tatttttaag ggtttttcta aaatattttt taaatatgaa gttataggtg cagagaatat
     4021 tccagataga ggaaatatag taatagcatc aaatcataaa tcgaatcttg accctatatt
     4081 cttggctgct gccatagaaa atagagaaat agctgcaatt gcaaagaaag aattgtttaa
     4141 agtaaaacca ttgggattta tattaaaaaa attgcatgtt atgcctataa atagagaaaa
     4201 accagatgta tctacaataa aaactatttt aaggtcagtt agagacggtt atgtattagg
     4261 aatatttcca gagggtacta gaataaaagg agattctttt ggtaaggcta aggcaggtct
     4321 ttctgtattt acaataaaaa gtaaatctaa agttgttcca gtatcaataa tatctaaata
     4381 taaacttttt agtaaagtaa ttgtatatat tggagaacca atatcatttg aagaacattt
     4441 taaggaaaaa ttgagtaatg atgaccatga aaggatatca caagagattt tagaagtgat
     4501 aaaacaaaat tactttaaat attcaaaata agggggtaaa gttatgaaca taaaaatagc
     4561 aaaaaatgct ggcttttgtt ttggagtaaa aagagcaatg aaaatggcat gggatgaagt
     4621 tgaaaaaaat gattctggca tatatgcttt aggtcctctt attcataaca agcaagcagt
     4681 agcaaaatat gaagaaaaag gtcttaagac tgtgaatgaa attgatacaa taccaaatca
     4741 tgaaaatatg ataataaggt ctcatggagt tcctgaaaat atatataagg aagccaaaga
     4801 taaaaaatta aaaattgtgg atactacatg tccatttgta aagaaaatac atacagtagt
     4861 aagtgaatat cataataaag gctatgaaat aatagttatt ggagatatga agcatccaga
     4921 agttataggt ataaatggat ggtgtgaaaa ttcagctata ataataaaga ccttagaaca
     4981 aatggaaaat atggaatttg ataactccaa aagatattgt cttgttgcac agactacaat
     5041 aaatccagag ttgtatataa gtatagttaa taaactatca gataaattag aagaaatagt
     5101 atttaatgat acaatttgtt cagctacaaa aacaagacaa gaatcagcta aagagttagc
     5161 taaaaaagtt gattgtatga tagttatagg aggtaaacac agttcaaata ctcaaaagtt
     5221 agtaaaggtt tgtgaagact tagtaccaac ttttgcaata gagactaaag atgaattgga
     5281 tgtaaatacg cttaaaaaat ataaaaattt gggtattaca gcaggggcat ctacacctaa
     5341 ttggataata gaagaagttg taacattcct tgaaaattta taatttgata ggtaataaaa
     5401 ataggtggac attgttataa tttatcataa tatccacctt tgatttcata ttacatgtgg
     5461 taaatagata acctaatatc aaaaagtgtg tatcttaaga tgaaaaacta agagtataag
     5521 atgcgcactt tttaatatta gaaattcaaa agaaatattg atattgatta tcaatagaaa
     5581 atagaatata atataattgt tgcaaaatta gtctaggagg taaattaatg gaaatgttga
     5641 tatccgcttc tgaagaggcg tttttacatg taggttctat gattggattt tttattttgt
     5701 tatttggata tattaattat aaaactagtg gaaattttac taatatcata tcaaaaaaca
     5761 gaaaatttca acccttaata ggtgctttaa ttggagctat accaggttgt ggcggaagtt
     5821 tggcaataat gccattgtat ataaatggaa aattaagctt tggagcaata attgcaagtt
     5881 taatagctag tatgggagat gcagcatttg tacttatttc atcaaatatt aaaatgtatt
     5941 tttttgtcac tatagttagt acaattacag gaataataac tggacaatta gtggattact
     6001 ttaaactaga agaaaaatta gggttaaaaa accgtagaaa atcagataaa tatgatataa
     6061 gtaaaaataa ccctaaagaa aataaagaac aacataatca tgatgaaata attttagaca
     6121 ctctagctaa atctcatgga aatacaaata gacttgcatt tataataaca catggaaagg
     6181 gatataaaat ttatatagga attatagtaa tcggttttat atttatgtca cttgctcact
     6241 ctggacttaa tcttcctata attgagaagt tacattcatt ggaagaagta atagcagtga
     6301 ttggaatatt attttcaata atttatatgt ggtgttttaa aaaggtattt aaaaattcta
     6361 atcaaaaaga ggaagaaaat aaaaagattt ctttgcgaga aatgcttatc cattctgttg
     6421 gagaaatatc atttgtaatt acatggattt ttattgcata tcttatttac gatttgataa
     6481 tattaatttt aggtggagat gaatacttag ttaaattagt attatcaaca ggtgtaataa
     6541 gtgtatttat aggtgcaggt cttggactta ttccaggttg tgggatacaa attgtgctta
     6601 tgtcatttta tttgaaggga actataccac ttggtgcagt gatagccaac tctatttctc
     6661 aagatggaga tgcacttttc ccacttttag caatggataa aaaatcttct ttatgggcta
     6721 tgattattac tactatacca gcaatacttg taggtatgat agtttatgca tttttaggtt
     6781 aaatataatt atgaataaag aagtattaaa aaaataataa agcacataaa tttatttata
     6841 agaatagtga agattaaata taaaaataaa aaattcaata tgaattaaat tattgcttaa
     6901 atattaatga gaacatggga gataatatta ttcctcatgt tctctctatt taattaaagg
     6961 aattatggaa caaatttcaa gtgatacttt ctatcaaaac tcatatataa tatatattaa
     7021 taaattaaac tataatacgt aatacagtca gtatattgat ataaaaaact taggaggagt
     7081 tgtaaaaatg ggtaatttaa aaagacagtg tgatgtttca agtggaagag aaaaagctga
     7141 cattgtacta aaaaatggga caatcataaa tgtatttaca gaagagttaa taacaggaga
     7201 tgtagcaata gtaggagaca ctatcgttgg aattggcgat tacaagggca atgtagaaat
     7261 tgattaaaaa atagctacgc acactcgtga ctttagtcat gagttagtag ctaaaatagt
     7321 cagcatatag ggaaacctat atgtagagat aggatagaac ctatccaata ctacttgaat
     7381 tgctggaaac ccctaaagct aatcaaacta aaacataagg atgaaataaa cctaagtgtg
     7441 aaagttacga aagtagaaaa aattggttag atggtataag gttaaaccct aagtacttta
     7501 aaatgggcaa tcagcaggta aactccgaat aggagaaact tcaacgacta ttcctcttga
     7561 gggaagtaca ctacaagcta ttggtagtgg aagtgggta
//
LOCUS       DBGJFU010000162         7582 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.165, whole genome shotgun sequence.
ACCESSION   DBGJFU010000162 DBGJFU010000000
VERSION     DBGJFU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.165"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(19..717)
                     /gene="yunB"
                     /locus_tag="ACOIAS_003389"
     CDS             complement(19..717)
                     /gene="yunB"
                     /locus_tag="ACOIAS_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HGZ2703057.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            931..3624
                     /locus_tag="ACOIAS_003390"
     CDS             931..3624
                     /locus_tag="ACOIAS_003390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HGZ2703058.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFKENNEWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            complement(3898..4833)
                     /locus_tag="ACOIAS_003391"
     CDS             complement(3898..4833)
                     /locus_tag="ACOIAS_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HGZ2703059.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4877..6022)
                     /locus_tag="ACOIAS_003392"
     CDS             complement(4877..6022)
                     /locus_tag="ACOIAS_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2703060.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            complement(6036..6500)
                     /locus_tag="ACOIAS_003393"
     CDS             complement(6036..6500)
                     /locus_tag="ACOIAS_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703061.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(6515..7357)
                     /locus_tag="ACOIAS_003394"
     CDS             complement(6515..7357)
                     /locus_tag="ACOIAS_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HGZ2703062.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
BASE COUNT         2585 a         1305 c         1067 g         2625 t
ORIGIN      
        1 aaaatataca actttatatt acttgttggt attctttcca gtgttaggta aaacatttcc
       61 tagatttttt tctggaatat ttacataact ttctggaaca tcacctacta ttatagtttc
      121 acaaataggt atttgagctt taatttgttt tgttgaagtt ataagtggta ttactacttt
      181 aacttgtgtt tgagcttcta aatatattct atgtctagtt tgatttatac ctgaagactc
      241 aaattccgtt ttaaagttta ctgatactgt tcctataggt tcaatagata cttcaagttt
      301 aggaccgtat ttagctaata taggacttcc taatgctgtt cctataggta tgtaagaact
      361 tgctgtctta tcctttttta attcatcttg tatttctaaa gctattgcag atgcaatttc
      421 attcatcatt atagtgtttg cttgtatcat agttatttta ccattattat caagttgtat
      481 atccattaaa tcttcataat ttatttttcc ttctaccatc tcagcaacag ctttatttat
      541 cgacctattt gctagttcaa gagctttagt ctctgctaaa actgttattg tcggtcttaa
      601 agttttatct atatatataa aactgcctat aaatacagac aagaaaagaa ttactaaaaa
      661 aatagctatg atttttctaa attcattttt tgtttttcta tctactattc tattcaaaat
      721 tatcccctcc tatttattta tatgtagctt tggcataatt tttataatat ttgtcaaaaa
      781 taaataaaat aaaaatatag actttttaat atattacgtt tatacatata caatgtacag
      841 aatattatag caaattcatt ttttctatta attacaaaac tactcctatt tgtgttataa
      901 tattgcatta agtaaggagg ttaaaatctt atgaataacg ataatgataa aaatggaaat
      961 aaaatcagga gaaaaaaagt tagctcatct ggtaatacaa ataaaccaat taatagaact
     1021 tcagctaaca agactcgttc aacaaaaaac aagaaaaaat ctaaaaaaag cgataaattt
     1081 aaaaaactaa gagtatttgg gatagtattt ttagttttac tagttgttgg tacagcaggt
     1141 actgctggtc tagtatttgc gtcactaaga gatgtaactc cagttacaga agctgtttta
     1201 gataaacaga cgaaccaaac aactactatt aaatacgcta atggaaaaac attatctaca
     1261 gctccaagtg taaataaaaa gacacccgtt ccattagata agatttcccc ttatcttcaa
     1321 catgctgtaa ttgcaataga agatgagcgt ttttatgagc ataagggtgt cgatattaaa
     1381 ggtttattta ggtcagtatt aaagaccctt acaggtacta agcaaggtgg tagtactatt
     1441 cctatgcagg tatccaaaat gctgttaaca actgaacagc aaactatacc tcgtaaaata
     1501 aaggatattt actatgcaca tgagatgagt aaaacagtaa gtaaagataa aatactagaa
     1561 acttatttaa ataacttctt cgttggtaga ggtctagcag gtgctgaggc tggtgcacgt
     1621 ggttattttg ataagtctgc agctgacctt actctagcag agtcagcact tcttgctggt
     1681 tcaactaaaa atccatcaag attttcagcc tataaaactt ctaagttaga gggaaatgaa
     1741 actaaggagg atttagagaa taaattatta ttttttgtta acacaactga tgatgattta
     1801 gatgacccta ctcaagtgga ctttgatatg attgaaaaaa taaaatcttg ggagcttata
     1861 tctaatgata cttatagaca attaaaagca ggtacattag ttgttagaaa ggctgtaagt
     1921 aatcctgaag ctaaaaaaag acaagaaatt gttttaaaga aaatgttaga attgaaatat
     1981 ataaaacaaa gtgaatatga tgaagcaatc aaagctaaaa tagaaataaa attgcctcaa
     2041 tcatcagata aagtttcttc atctgtagaa gaccttatcg aaagtgaagt tataaatgct
     2101 ttaatggaac aaggacatac aaatgatgaa gctcaaaact tattctacaa tggtggtcta
     2161 attgtaaaca caactataga ccctaaaatg caagatgctt tagaagaaga atttgataga
     2221 aatagcaatt tcccaggaca tatggtcggt cctgatggag ttagccaacc gcaagctgca
     2281 atggtaatct tagattataa aaatggtgaa ataagagcac tagctggagg aagaaatata
     2341 agtggtagaa aaactttaaa tcgtgctact aaccctcatc agccaggttc ttctataaaa
     2401 cctttagcaa tatatacgcc agctattgat actctaaaaa taacacaagc aactgctcta
     2461 agtgattctc gaggaggata taagtttaaa gaaaataatg aatggaatcc aagaactact
     2521 actgctggtc atggttctat gagtttacgt aaagcacttg ctaaatcttc aaacacgata
     2581 gctgtcaaaa ctgctgaaat gcttggagac tcttatgatg aatgtgtaga tataatgatg
     2641 gattacttaa agaactttgg tataacaact ttaaagaata atcactcagg ttcatctgaa
     2701 gcaagtgata gaaaattccc atcattgaca ctaggtggta tggcgaatgg tattactcca
     2761 cttcaaatgg ctgctgctta tggtacattg gcaaatcacg gtatttatgt agaaccttct
     2821 atttttacta ctataactac ctttgatggt caattattag ttaaaaatgc tcctgaagaa
     2881 cataaagttg tagaccctga agtagcttat gttgttacag atatgttaga aagcgtaatt
     2941 actgaaggta ctggtggtgt tgctacactt ccaaagggaa tgcctgtagc aggtaaaaca
     3001 ggtactacaa atagtgcata tgatgcttgg tttgtcggtt acactcctta ttatgttggt
     3061 gcaacttata ttggagatga tgctggtaga aaagatgatt ctggtaacac tataaaacgt
     3121 agagaagtcc cacatggaag tactagtact gcaaaactat gggaaaaaat tatggaaaaa
     3181 atacatgcta atttaacggt tactgaattt gaagttccta aaaatgtata ttttacaaaa
     3241 attaatttag aggatggagg aaagcaatct tctggctcta aagcagcatt tattgaaggt
     3301 actgctccta ctaaagtaag ttctcaacca tcatctgaag atacaaaaaa accagacaat
     3361 aaccaaccag aagaaaataa taataacaat aataacacag gtgataatgg tggcggttct
     3421 acacctcctg ataatggcgg aaacaatggt ggtggctcta ctactcctcc tgataatggc
     3481 ggaaataatg gtggtggctc tactactcct cctgataatg gcggaaacaa tggtggtggc
     3541 tctactactc ctcctgataa tggtggaaat aatggtggtg gctctactac tcctcctgat
     3601 ggtggtggaa ctccagcaac ttaataaaaa atagaattta aataaaaata taaggagttg
     3661 tctcaaggtg attaaaaaaa tcactttgaa acgctccttt ttttataatt acaatatatt
     3721 atttgtattc ataaaaaaga gaatcccaat aaccagaatc ctccttttta tataaacaaa
     3781 taaatatttt atttttaaag tttttaacta agtgctttat tcttactttt attcctaatt
     3841 gtttatatat tcttaaatag ttatatcaca aattatataa acttaatatt tttctaatta
     3901 ctgtatacca aatgtttgaa atctatttaa tttgccatat ctcttaataa gcatttccca
     3961 ctcttcttta tcatagaaat cacacttatt tctaccaaat agcttggcaa cttcaattgc
     4021 aaatctccca gctgaatcta aatcactaaa attagttgca cctgtagcac atccagcaac
     4081 aggcatctct gttgtaattg ctactcctac tattggtgca tctgtagcta ctgctggttg
     4141 taaaatacta tttaagtgat acagattatt tccatatggt gttatatctt gaatagacaa
     4201 agggaataca aatggagatt tccctgttac agtctgcata atatctaaaa ggttatcact
     4261 agtttttaat atgtagcctt ctttaacagt tggcgatatt gcaaatcctc tagtattgat
     4321 tactctattt cctttagtag tatctataga aagtattgca tcacaactac tatcaacttc
     4381 attttcattc atagtcatca tatcaattgg agaatccata aaaggagtag gatagtgttc
     4441 tttagttgga gcatctgggc aaatatgggt tgaaattaca acatcaccat ctaatatatc
     4501 cccttttcgt tgcatatcca aaagtttagc agccactgca attgctacca atgctccatc
     4561 accatctgaa acaaaaccta taacttctgg tcttgctcca atccctccta gtctacctaa
     4621 aatgccaaga gtaggagcat ctccaccttt taattttcca tttttcccag gaattgtaag
     4681 ctttactaaa tctgttgagc ctttacttga agacaattct tttacagtta catctacttc
     4741 accataacct tgtaagtaag tttttacttc ttccccatta gcatttgctt tatctaaaat
     4801 gtcataaacc tctattactt gttttaatat catgtctaat ccgatttcct ttcttaatat
     4861 attttaaaat tatatattat ttactataca aaatatctac tccacacatt gctaacacct
     4921 taattgcttt tataagtgct actctgcctt cttcaccacc agctgcaaca gcaaattcat
     4981 ctgtatgagt tgcagtattt tcttttaatt ttatatatgc ttgtattgca ggtatctcat
     5041 gagttaaatt tcctgtatca gttgaaccta taccttgact taaatctctt tcaacatagt
     5101 cttcacccaa aacttcaaaa ttttgtctaa ctatattcac taattcagaa tcattcttaa
     5161 tctctttgta tatttcatct aattgaacta tttctacatt agttccagta gaaattgcag
     5221 ctcctttagc acaattttta agcatttcac aaatctcatt taaatattct atgcttaaag
     5281 ctcttacatt aaatatagct gtagattttt caggaattat attatgtatg ctacctccat
     5341 cagttataat tccatgtact cttgcataat ctttaagatg taatctcatg ctatttacac
     5401 tgttaaatag tgctattact ccacttaagg cacttttccc ttcccaagga aaagcagcag
     5461 catgtgatgc cttaccagta aaatcaaatt ttaaattaac ttgagcaaat gatatatcat
     5521 caggcattga agcatcactt ggatgtaaaa ttaatgcagc atctacatta ttaaatacac
     5581 cttctttaat catagttatt tttccaccta ctctttcttc tgcaggtgtt ccaaaaacct
     5641 ttacagtccc ttctatatta tgttttttca tgatttcttt taatatgata cctgcaccaa
     5701 cagaacttgt ccctatgata ttgtgcccac atgcatgtcc cattcctgga agtgcatcat
     5761 attcagctaa aaaagcaact gttttcccat tagttccatt tgaatacaca gcttcaaaag
     5821 atgtttctaa tcctcctaca cctttagtta cattaaattc attttcttct aacaaatcac
     5881 ataatgcttt ttgtgactta tactcctcaa atgctaattc tggatttgaa aataaatagt
     5941 cagaaacttt ttcaaatgat gtctttttct cttcagataa taactttatt tcctctttaa
     6001 tttttgcttt taattcattc atatttacta cctccttaaa gcattccatt taatcttaaa
     6061 actgtttgag atataaaagc agaaccaaag taagtagatg tcataactat aaatgatata
     6121 actactaact tccaactcaa agtctttaat atatctaatt ggttacctac agagattcct
     6181 gcaaaagcta ataatggtgt tactgtagac ataaaattga ttttccctac atttgttgct
     6241 atgatatccc ccactggaga tattggaagc gataaaacaa atgcaactaa tgtagcccat
     6301 gcaaaacctg gtaaattagg ttttttaaca aaattcttta ttataatagc aattatcacc
     6361 aacaaaaatc cagatacact tccaatcaac aaatcttgag cctgagtctt aagtgctatc
     6421 atttgtccaa agcaaataat aatatatata aacaatacta tttttatctg ctccataata
     6481 gtcttaaaag ttttttccat atatatatcc tcctttacaa tttattactt gttcttcttg
     6541 tttttcttcc tctaaatctt tcaataactg gagctacttt gctataatag tagttggcta
     6601 ctggtaaaga taagaataat tctatatata ttccagtaac agctgtaatc aatccactag
     6661 tagctgaata tgctaaaatc tgctctgaca tttgtggaac cgtattcatt aatgatgctg
     6721 ttgcagctcc catcattgaa ccactaccca ttcctgatgc cattgctaaa gcatatggat
     6781 gaaatcctaa aagtacaccc actggtgcta aaaatgaaaa taataatgtt ccaaatatac
     6841 taccacttat gtaagttcct agtgttccta taccttctgg tgagtttaat ccatacttat
     6901 ctccaataat tcctagtgct gtgtcacgac ataatgaaaa agtaccacct aatgcacttc
     6961 ttccaagacc taatgctact ccaacaggta atgctatcaa cattgtaccc atatgaccaa
     7021 attcttgtaa taaaagtgct ggtcctgctt gaagtaatat accaacttta ggccctgcat
     7081 taattccaaa cattatccct aatggatata gtgtcaaaga taataaatat ggagatacat
     7141 ttatttcatt atcccctata aactttttta aggctggtat aactctccct aataaatgag
     7201 gagaaacagc cattcctatg aatatagccc atagtatact aagtaaagtt acattaattc
     7261 ccataacttt aaatgaaata ggtccaatta attctgccaa tattgtgatt ataaaggctg
     7321 ctattatggc atttctaatc cctgcttttt ctttcatttc tatacctcct tgtaattttt
     7381 ataattttct tataatataa ttgttagtga ttttattata taacctatca ttgataatgt
     7441 ataatatata tttattcatc atttttatat acttttagtt atgaatcaaa aaaatattat
     7501 agattcatga tataaaatac aaataattta ttaatttata ttgttaaaat aacggatttt
     7561 caaataattg aataaaatta tt
//
LOCUS       DBGJFU010000163         7543 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.166, whole genome shotgun sequence.
ACCESSION   DBGJFU010000163 DBGJFU010000000
VERSION     DBGJFU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.166"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(749..2200)
                     /locus_tag="ACOIAS_003395"
     CDS             complement(749..2200)
                     /locus_tag="ACOIAS_003395"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGZ2703063.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKIKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(2216..2656)
                     /locus_tag="ACOIAS_003396"
     CDS             complement(2216..2656)
                     /locus_tag="ACOIAS_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGZ2703064.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            complement(2904..3332)
                     /gene="ytfJ"
                     /locus_tag="ACOIAS_003397"
     CDS             complement(2904..3332)
                     /gene="ytfJ"
                     /locus_tag="ACOIAS_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HGZ2703065.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            complement(3286..3915)
                     /locus_tag="ACOIAS_003398"
     CDS             complement(3286..3915)
                     /locus_tag="ACOIAS_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HGZ2703066.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            complement(4077..4616)
                     /gene="scpB"
                     /locus_tag="ACOIAS_003399"
     CDS             complement(4077..4616)
                     /gene="scpB"
                     /locus_tag="ACOIAS_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HGZ2703067.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(4618..5337)
                     /locus_tag="ACOIAS_003400"
     CDS             complement(4618..5337)
                     /locus_tag="ACOIAS_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HGZ2703068.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(5367..5963)
                     /locus_tag="ACOIAS_003401"
     CDS             complement(5367..5963)
                     /locus_tag="ACOIAS_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HGZ2703069.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            6261..7097
                     /locus_tag="ACOIAS_003402"
     CDS             6261..7097
                     /locus_tag="ACOIAS_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HGZ2703070.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            7118..7540
                     /locus_tag="ACOIAS_003403"
     CDS             7118..7540
                     /locus_tag="ACOIAS_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HGZ2703071.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
BASE COUNT         2555 a         1203 c          768 g         3017 t
ORIGIN      
        1 ttttcttttt tctagtgaac aatttgtaag tctctttata tttggattat ttttatacta
       61 ttgtccaaaa ctaaccaaaa atattttacc ttatagctat acagttgaga agataatttg
      121 tacattactt gtaataatta tggctctaga gcaattatta cttatttctt caggtaatta
      181 tagtactcta aactctttac caattggaat caattatatt tgtatatatc tgtgtatagc
      241 catactaatt tttaagcagt atcatctttt taatatattt ttttcttgga gtttagtttg
      301 ttcagttgga gaactcattt tttctaaaaa cttgggctat gaatttccta gcttaatata
      361 ttttatcttt atcttctcta agtgcttgat aatttatgca gatatatata tggttgatgt
      421 tagaaaattt agagttaaca gatatgcact tagagataac ttagctatat gttttattta
      481 tttttcattc atatttttac taaacacatt caccaactct aggtactatt atggtttttt
      541 aagtcatagc acaactgcta tttttacgtt tatttttgtt actagtatta tgtacatacc
      601 tgctctttta tttaatagag atacttttat acttgaaaaa aagaaaaaga gtaaataatt
      661 tatttaaata aaaaataaag ccacatagat aatcttaaat tacaaaaata tccacgtggc
      721 tttataaatt atattttata attgtttttt agatttctgc caaatagtac aatgttctaa
      781 caggttctcc tgtagccccc tttgctttgt atccaattga ttttgatgat gtacttgtcc
      841 ccgctatatc catatgtatc caaggagtat tcccaacaaa ttcaccaata aaagctcctg
      901 ctgttataca tccagcattt ttaccaccag tgtttttaag gtctgcttct tctcctttta
      961 tcatatcttt aaattcatca taaattggca tcttccatac cctttcccct gataaagtgg
     1021 cagctttttc taattcacaa taaaaatcat cattatttgt tactatagga gttgcaacat
     1081 tacctaacaa tgttaaagca gcaccagtta atgtagctac atcaaccacc tttgttactt
     1141 tttcattgtt tataatgtag tatattgcat cagctaatgt aagtctacct tctgcatccg
     1201 tatttaaaac ttcaatagtt ttctttgcca tagaactaat tatatctcca ggtttgtatg
     1261 aacctcctga taaagcattc tcacatgcag ccactactgc tataacattt ttctttaatt
     1321 tactttgaga tatagcacac atagcaccta taacactcgc agcaccagcc atatctgtct
     1381 tcatatctag catagaagca tttgatttta aagaatatcc accagtatca taagtcaatc
     1441 ctttacctac taaacctaat atatctgtac tttctttatc tccaaagtat ctcattacta
     1501 tgaatcttgg tttattaatt gagcctcttg aaacagcgaa aaaagcatcc attcccaatg
     1561 cctttatttt ttcttcttca taaacatcaa cattaaaccc aaactctgca ccaagtttaa
     1621 ctgtttctac tgctaatgtt tttggatata ttacgtttga tggttcatta actagatttc
     1681 tagctattat agttgaatta gctaatattt tagccttttc tatttgacac tcaacttctt
     1741 tttcagtaaa tatagttata gtatcaactt cttttctttc aaaatcctta atttttgtct
     1801 tatacttatc aaacttataa tttccaagta atatcccttc tacaaaagaa cctatttcat
     1861 cactatttaa attaagattc agtacattaa catcactact cttatatgct tcatttttca
     1921 atatattttt taatttttta cacaaattac cacctatgat tctaattttt tctctattca
     1981 ataactctct cttaccaaaa ccaacaagac aaacatttac caaattgtta ttttcaatca
     2041 atgttacagt atctatacta ttttctttag aattaaaatt tctgcttatc atatttattt
     2101 tatcaaatgt cgtagaaaca tcttctccct caaatagagc tactactgtt atagcatttt
     2161 ctaaactttt taatatttct ttagaatact ttatattcat aataaattct cctttttaaa
     2221 ataattgttc tattatattt ttagactctt ccaaagaagt aaataattta tctttattat
     2281 ctatatctag gcaatccatt ttatcactta ttatagatac aaactttagt atttcattat
     2341 ccaatatctt ttctccattt ttagttattc cataaaaagt atttcgtcta tctctactat
     2401 cttcttccct atatacatac tcttgctcaa ccaatttatt aagtagtata cacagactag
     2461 aagtagatac atgcaaatct ctacttagat ttttaagctc tattttatca tgttttctta
     2521 tttttactaa ggcaaagaac tgtctttctg taagaacctt atctttttta tatctttcgt
     2581 tattaataat ctttattaaa ttatgaaaat tattcatact ttctaacaac aaaatagata
     2641 attctttatc gctcataaca ttcacctcaa aactaatgat ttataactcc ctcttatatc
     2701 tcaaatgcgt ttatatatct ataatataat acttcttttc tttttataat tatactatat
     2761 atcaacttta ttatctattt aaattgtttt aaactaaaat cttattagat ataaatgttt
     2821 aaagtacaca acaaaatagt gggatttata ctaaccccac tactttacct aaaactatat
     2881 acatatttaa aaatatttta ttcttacata ttgtttttat cttgattctt attactttgt
     2941 gatatatttt gaattttctc caatactcta ggagttatag ataatatatt atccactaga
     3001 ttgatattac tgtctaaact cattattcta gtctctccac tttcaactac tacaaatgct
     3061 actggttgta cagatatagc ccctgcgcta cctccagcaa aattcgtatc tgatttttca
     3121 ttttttaatt caatttccct atctttatca tcatatcctt ttgagtattc tcctcctcct
     3181 ataccaaagc caattgtaac ctttgatata ggtacaacca ctgtagtatc tgtttttata
     3241 gggtctccta taatagtatt tgcatctata gaacctttaa ttgtttcaag agttgtttcc
     3301 ataagactct gtatcgaacc tgttgctctc attactatca ccttccttat ttcctttatt
     3361 tttattcata attttattaa tcattattat aatattaaat aaagctttta ttcttggcct
     3421 tatatgtatc cttatatttc ctaaaacata atcttttgta aaatctggat ttacatttaa
     3481 atacattttt tctgcatcaa tcatattaaa taaatttcca tacaaagtat ttatcaatac
     3541 atatacagaa gatgttaagc caatatttcc agttccaaaa ttaatatttg aataaagttc
     3601 atgtattttt atctttttta ataatctata aatgctcaaa aggtcatcag ctaataatat
     3661 acttgaatct atattattct tcatcccctc ttttttatca ttatttttac tattttcagc
     3721 tggataaagt tgtctattta tatttattaa attaaacatg tattttatat taagctttaa
     3781 acaaatatct ttgtttttta tatcagctat tattaagatg tttatatgag atattaaaat
     3841 caacatagtt acacttataa taaaaattac agctattatt aaatacttaa aattaaataa
     3901 ttcattaagc tccatgttta tattatcatc aaaaaattac tatttatact taattatcca
     3961 tatttatttt aagagaatta taattttata tttatacaaa aaaacacaac aacttagagc
     4021 attttaaatc ttttaaaatt taaaattaca tactaaatta ttgtgatttt atatttttat
     4081 tcatttgttg ccacttcttg ataattttct ataggtggca attcttcaag actttctata
     4141 ttcaacaact ttaaaaattc atctgttgtt ttataaatta ttggtttccc tatcttattc
     4201 aatctaccag cttctcttat aagttcattt tcaaacaaag tttgtaatac cttatcacat
     4261 tttactcctc ttatatcctc tatttctacc tttgttattg gttgtttgta ggcaattata
     4321 gtaagagttt ctaatgtagc ttggctcaaa cttttctttt tctttggttc aataatcttc
     4381 tttatatatt ctgcatagtc tttatttgta cacatttggt acttattctc taacttgatt
     4441 atttgaatac ctctattttg ttctctgtac tcctcaataa gtaaatttaa cataatctct
     4501 atttcttttg atgaaagttc tttatttatt atatagttta attcttttat acttattggc
     4561 tcaccatatg caaacataac agattctata atatacttta tatcttctct tttcatttta
     4621 acactccgaa ctttttctta ttagaatatc atcaaaaaat atatcttgta ctacgactat
     4681 ttctttttct tttataagct ctaatataga taaaaaagta gctattattt catcattttc
     4741 atatgatttt ataatatttg taaaagtaac ttcaatcttc tcttttataa tatctcttat
     4801 atataatatt ttttcttcta cagatattat tttcttcctt actattttat ctagtttctc
     4861 atcattagca ttttctatct gtgatgtttt aactttaaat atgtatggaa gtatattttt
     4921 tattgcttca attgatatat cttctaaatc cacattgtca tcaataatta tctcttcttt
     4981 atttctataa aatctctcat taacataagt tatattttct tttatatctt gtgatgctac
     5041 cttaaacttt ctatattctt ctaacttttc cataagttct attcttgggt cttcttcttc
     5101 atctttttgc ttataaagca gatatttaga ctttatctct aaaagttttg atgccatagt
     5161 tataaattca ctagttatct ctaaatccat tttatctagc attttcaaat aatttagata
     5221 ctgcttagtt atatctatta tggatatatc ctttatatct attttatgct ttgttattaa
     5281 atcataaagt aaatctaatg gtccctcata gacttgtaat tgtatattat acttcatatc
     5341 aatccctctt taatctgttt attatattaa acaaataaat atactatttt tagcaacact
     5401 gcatatattg gtgttaatat tattctatac ataccagtaa ataaagcaat taataaaatt
     5461 atataactca tactttcaaa tttatacaaa tattcattcc atttatatgg tacaaatgta
     5521 gatataactc cccatccatc aagtggaggt atcggaagta agttaaaagc tgcaaatcca
     5581 atactatata aaaacacata ttcagctatt gtatttatag catacatatg cacatactta
     5641 ttgataatta cacacagtat agcagtcaaa acattacaaa aaacgcctgc aagtgaaact
     5701 atcaggtttc ctatcttata gtttttaaag ttatttggat ttactggtac tggctttgcc
     5761 caccctatct taaggataaa catagccaaa agacctattg ggtcaacatg tcttacagga
     5821 tttaatgtca ttctaccata agctttagct gtatcatctc ccaataagtg agctgaataa
     5881 gcatggccaa actcatgtat tgatattgcc atagctatac caactaaact tgctactatt
     5941 tctcctatat tcatacttaa cattaatgta catctcctaa attaaatttt taatattatt
     6001 ataacaatta aatagcttat aatcaaaatt attttacatt ataataattt taacttctag
     6061 gcataaaatt aaaatttgct ttttatttta taacttttat tattatatca tattcattgt
     6121 gaaaatataa taattacacc tactgtaagt gtaatattat attgacatga attgacatta
     6181 ttgtctctta gtcaatatta tacaagaagt ataaaaaaat atgtgttaca ctttgtataa
     6241 catgaaagga ggttacctaa gtgagacaag aagaacgcac agtatgcttt gatagggaat
     6301 taaaaattga agcatataga tttaaaggca taatgcaaaa attcccaaac cattttcatg
     6361 aacattatgt aattggtttt atagaaaaag gtcaaagata tttatcttgt aaaaataaag
     6421 agtataccac aagtactgga gatttgctac tttttaaccc ttttgatagt catacttgtg
     6481 aacaaataga tgataaagta cttgattaca gatgtattaa tattaagcca gaaataatga
     6541 aaaaaaccgt ctttgaaatt acaggtaaaa attatttacc aaaatttaat caacctgtta
     6601 tatttcgcag tgagttagta cctctactac aagaattaca ctatataatc atggaagaag
     6661 aattggattt taaaaaggaa gagctttttt tcttcctaat tgaacaatta attgaagaac
     6721 atacagagcc aaatttgcaa tctaacttgg aaaatacaaa tatagaaatt caagcagtat
     6781 gtgattatct agaaaataat tatgctgaac atataatact tgacgaatta agcactatag
     6841 caggtatgaa taagtactct ctacttcgta attttacgaa attaaaaggt attacaccat
     6901 atagatacct agaaaatata cgggtaaaca aagctaaaaa gcttttagaa aaaggcgtag
     6961 aacctataga tgctgcgata caaacaggat ttgtagacca aagtcacttt acaaactttt
     7021 ttaagaattt tattggtctt acaccaaagc agtatcaaaa tatatttata aatgatgatt
     7081 caaacaactt taaataaggt aaaaaggaga aataaaaatg gaaaataaaa attcaaaatg
     7141 tgtcatggta atagacgaaa atttaccaat gggaattata tccaatacag cagctataat
     7201 gggaattaca ctaggaaaac atgccccaga aactgttggg ccagatgtaa ttgacaaaac
     7261 aggtaatagc cacttaggaa ttattgacat tcctgtacct attttaaaag gaaacaaaga
     7321 aataataaaa gatttacgta aaaaattata tactttggaa tttaacgatt taactgttgt
     7381 agatttttca gatgtagcac aaagttgcaa tctttatgaa gaatttacac aaaagattgc
     7441 tagtgttcca gaagatgagt tacagtattt tgggatagca atttatggga ataaaaagaa
     7501 agtgaataaa cttactggta gtatgccttt acttagataa ttg
//
LOCUS       DBGJFU010000164         7300 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.167, whole genome shotgun sequence.
ACCESSION   DBGJFU010000164 DBGJFU010000000
VERSION     DBGJFU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.167"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(251..1090)
                     /locus_tag="ACOIAS_003404"
     CDS             complement(251..1090)
                     /locus_tag="ACOIAS_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGZ2703072.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1114..1872)
                     /locus_tag="ACOIAS_003405"
     CDS             complement(1114..1872)
                     /locus_tag="ACOIAS_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGZ2703073.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1919..2392)
                     /locus_tag="ACOIAS_003406"
     CDS             complement(1919..2392)
                     /locus_tag="ACOIAS_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGZ2703074.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2421..2837)
                     /locus_tag="ACOIAS_003407"
     CDS             complement(2421..2837)
                     /locus_tag="ACOIAS_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGZ2703075.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2884..3936)
                     /locus_tag="ACOIAS_003408"
     CDS             complement(2884..3936)
                     /locus_tag="ACOIAS_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGZ2703076.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4332..5012
                     /locus_tag="ACOIAS_003409"
     CDS             4332..5012
                     /locus_tag="ACOIAS_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGZ2703077.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5145..5465)
                     /gene="sugE"
                     /locus_tag="ACOIAS_003410"
     CDS             complement(5145..5465)
                     /gene="sugE"
                     /locus_tag="ACOIAS_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGZ2703078.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     regulatory      complement(5535..5641)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            5847..6674
                     /locus_tag="ACOIAS_003411"
     CDS             5847..6674
                     /locus_tag="ACOIAS_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HGZ2703079.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2472 a         1317 c          850 g         2661 t
ORIGIN      
        1 ttaaataaat ttatattttt ttgaaatcac ttaatatttt atataaaaat gaaaaagcac
       61 ctcattctga ttaaaaatct ttctgagata ctttttaaaa tattaaatac tacatatagc
      121 ttacataagt ttaaaattta atctattata aaattaccct aattgccaaa ctttttaata
      181 taaattttcc aacatcctac aaatataatc aatagatata gaatcctaac ttacaccaat
      241 atatatttat ttaaaatagt cctaaaatac ctgtaatagc tcccactact atcagtataa
      301 acattatttt tattggagaa agaccttttt ttaaagcaaa gagtactaat aaagtaagta
      361 ccaaaggaat taacccagga aatatcttat cgagtaaatc cgtttgaaga ctaaatttaa
      421 aatcaccact agtataactt attggtgtag ataaattaat aaacctacct acaagtcccc
      481 ccatgactaa ggtacctagt atagaagccc cttctataac cttatttact attccacctt
      541 gaagtatact ttcaacggct tttttcccat acttatatcc atttgtccac attccaaaag
      601 aaattgaagt cattattata tattgagcta ttacaaataa tataggtcct gcaattgatg
      661 ctcctccctc agttaaacca atacaaacag ccaacactat aggagttata actccttgag
      721 ttaaagtatc gccaattccc gctagtggac ccatcaagcc tgttttgata gcattcatac
      781 catctccact tatttcatct gctccattag ctttttcttc ttccatagct attgttattc
      841 catgtataac acatccacat ataggttctg tgttaaaaaa tgctagatga gtctccactt
      901 cttgttttaa ctcttcctta tctggatata attttttaag tactggcaac attgaatgtg
      961 aaaaagctgt tgattgcaat ctttcataat tgtaattaga ttgtgcaaag aaaaaccatc
     1021 ttaaccatga ttttattaca tctttcttgc ttaacgtttt cttagatact tcatttttta
     1081 tatctgacat aatattttcc ccctcacatg tatttaagat gtagcactat cttttccgta
     1141 cttaattgaa gtaattgtaa gagcaagtat tactccaata acagatatta ctattatatc
     1201 tactttaaaa taaacagcca ttagtattcc taatatgaaa aagggcatag taaacttgtt
     1261 tccaatcaat tttaaattca tagcaatccc aagtgctggc aacatagccc caattacatt
     1321 aaatacatgt aacacattat cattcattac ttccaacata ccatctatcg catttggacc
     1381 aaaataaaca actaataatg ctggtataaa actcattgca aataacagta tctgtggtgg
     1441 tactatattt ataaatgcta ctctatcaac attccctttt tgaacctctc tatcagccca
     1501 atgtgcaaaa aaagaattta ctgtcatctt tccaatccaa agtacagttg caacagttcc
     1561 caatggtaca gctattgcca ttgcagtact tacatctagc ttagatgcta tagctaaagc
     1621 agtacctaca tatcctgcaa aagctgggtc ccccatctga gctccccctg ctgatataaa
     1681 acctagatat atcatattta tacctgctcc tataattgct ccttgaacag ggtctcctaa
     1741 tataattccc gttacaaatc cagccaatag aggtctattc catgtaaaaa atccaacata
     1801 agtcaaccac ggactccaag aaagataata aaaaattgct attaatactg cttgaattag
     1861 tgaaatgtgc atattcttcc tccctatttt cttttaaaga tttacaagat agtcttcttt
     1921 acaataatgt tcctacatca attttctttg catctggtac tatttgtata aagcatttaa
     1981 catcttttga tattagctct ctaaatattt ctttttcttc atctgacaca gatatatttt
     2041 tataaaaact ttttctacca agccctgctc ccatacctcc aacattcaat tcctttaaat
     2101 ctaccccgct atttactaaa cctaagacag ttttagggta tttgaccaat actattaaat
     2161 tttcatctcc attatatgat tttaagactt cagcacctga ttctacatca tgtacttcaa
     2221 ccttaacagt tgatggagct gccatttgta aaacttgaac tataaatggg tcctttacta
     2281 ctaaatcatc cacgattact atcctttttg ctgatgtaat cttacaccaa gcagtaataa
     2341 cttgcccatg tattaatcta tcatcaattc ttgttaatgc tatattactc ataattattc
     2401 ctccagattt ttaatttatt ttacaacttt aaaacagttt taacatcttt gattcctgat
     2461 atcccaactt cctcacataa attcttcaag tcttttaact tcatagtatc tctgtttaaa
     2521 aataactcta aaagcatagg taaatttaca cctgtcaaac attctaactc acatacctta
     2581 gcaaatttat ttattataat agcagatgta ttaaatggag ttccgccata aaaatcaagc
     2641 aatactaaaa ccccttcatc cttatatttc tcaattccat cttctaactt tctactaaac
     2701 tcatctatgc tgtcaccatg gtttaatcct aaagtttcta cattttcctg tttgcctact
     2761 attaattcag cactttctaa cattgcttta gccatatttc catgtgtggc tataattatt
     2821 cctgccatat gaatcatacc tttcttaacc ttttttatat ggattattat ttagtactct
     2881 aatctaatta tctatattaa tcattatctt accaaagaaa atatcccttt tgtatacttt
     2941 ttcaaaataa gctggtactt cttctaaatt tattctatgt gttactaatt tttcaacatt
     3001 catgtcaccc ttttgcatat gatacaatcc agcactccat tcttttcctg ggaatggaaa
     3061 ggagtttcca aaccaagttc cctttacagt taattcactt cttactatct tttcaaattg
     3121 ctctctagtc aaagctacat ccccatatgg tacacctgca tacaatactg tacctccctt
     3181 tttagctagc aacagtactt gaccacatgt cagtggagta ccagccgatt caataacaat
     3241 atctgcacca tctccatcag ttaacctttt tatttcttct acaatattct tttcttttgc
     3301 atttatacaa atatctgccc ctaactcttt tgccaagtct aacttctcat caaatacatc
     3361 cacagctatt atttttgtag aaccaaatat ttttgcccat tgtattgaaa ataatcctat
     3421 tggacctgta ccaagtacta caacagtatc tccaacttta gcttctgacc taaaaagacc
     3481 atgtcctgct atacatactg gttctagcgc agcggcagtc tcataactta tctcatctgg
     3541 tatttttatt aaatttctct ctttcacctt tgtatactct gcaaaacaac ctccaagttc
     3601 tttatttcct attattgcta cattattaca tcttgaatac aagccttttt tacattcatc
     3661 acattcaaaa catggcatag ctggacaaac agcaactcta tctcctattt taaaactcct
     3721 tacttcttta ccaacctgtg ccacttctcc agaaaattca tgccctaaaa tttctccaac
     3781 catatgtgga cctgtctttg aatattttga tatatcagaa ccacatattc cagctacttt
     3841 tactttaatt attacatcat ctttttctaa tatttttggt acatccacat cctccactct
     3901 tgtgtctctt ataccataga atctaactga cttcactatt aaccacccct actttttatt
     3961 tgagaatata tccctattgt ttatatgtta aatatctaaa gcaactttca tgccaatata
     4021 tgtaagcatt gtattcatgc attttataaa attatcatta aatgtgtgtc aaagtgtgtc
     4081 aaaagtttct tatattaaaa cttatgtgtg tcaatataaa cgacactaac attttatatt
     4141 agttctctta ttataagttt tataaaatgt actttttttg ttatttttat aaaattgttc
     4201 ggaaatttcc cttctatttg tgatataata ttaaatataa ggtatcaata taaattttca
     4261 tgcgaatatc agaacttttt tgacactaat gccttattca atataataat ttaacttttt
     4321 ggaggttgtt tatgaaaaaa gtgtatcaag gaaagactaa agatgttttt gaattagata
     4381 atggaaatta cttacttaaa tttaaagacg atgtaactgg taaagatgga gtatttgacc
     4441 caggcgaaaa tagtgtaggt ctaagtatag atggaattgg tagagctaat ttagagacat
     4501 ctgttaagtt ctttgaaatt ctaaacaatg ctggaattaa aactcattat gtaagtgcta
     4561 atttagaaga agcaactatg gaagttctac cagcaaaagt ttttggtaat ggattagaag
     4621 ttatttgtcg ttatagagct gttggtagtt tcttacgtcg ttatggttca tgtgttgaag
     4681 aaggtgctaa actagattgt tacgtagaag caactttaaa agatgatgac cgttgtgacc
     4741 ctcttataac atctgaaggt ttagaagctt taaacattat gactcaagct caatttgatt
     4801 caatgaaaag catgactcaa aaaatttcta acatagttcg cgatactata gctgaaaaag
     4861 gtcttgagct ttatgatatt aaatttgaat ttggattcta caatgatgaa gtaatcttaa
     4921 tagatgaaat agcatctgga aatatgcgtg tttataaaga tggtgtaata gttgacccta
     4981 tggatttaac tgctctttta ttagataaat agtatataca tataatttac aaatataata
     5041 aacttataaa taagataagc tatgaagtaa agacttttta aatgttgatt atttttaaac
     5101 attttctcgc ctttaatttc atagcttatt taaattaata tactctaatt agtagataat
     5161 agctttaacc ctacaattcc caccacaata aaacctatac atgcaattct tgatatacta
     5221 ataggctcat ttaaaatagc catgcctaca atagctgtac cgacggtacc aattccagtc
     5281 catattgcat atgaagttcc aagtggaaga tgttttagtg ctaatgacaa aaaataaaaa
     5341 cttacaatca ttccaacaac agtaagtata cttggagtta atttagaaaa tccatgtgaa
     5401 ttttttagtt gaaaagccca aaacacttca aacaaacctg caacaaacaa aaataaccat
     5461 ttcataaatt catccctctt ttcaaatatg tctcttattt ttccaataaa ataacctgag
     5521 aaatattata aaatatctcc caggtttttg tccttccgtg ttcacaacaa aaactatgtt
     5581 gtgggtttta tctcggacca gaccaatcat taataattgc ggaaccctat aaaactctat
     5641 taattattct attttatcta taataatact atcaacatat ataaaaaaag tcaatatatg
     5701 ggttccatat ataataaaaa taacttttat atacctttta atattcttat tcacttctct
     5761 ccatacccat ttgggtcaat ctaagaatat ctaattttta tttaatatgt tataataaca
     5821 taaaaatatt gaaaggagta atttctatga atttctataa aattgcagta tgtcagatga
     5881 ttactacaga aaataaaatt gaaaatatca atcatgctgt agatatggta acagaagctg
     5941 ccatcaatgg atctaaaatt gtagttttgc cagaaatgtt taactgtcca tatgaaaata
     6001 aatattttcc taaatttgct gaagaatatc caggtgaaac aacaacaata ttgagtaaat
     6061 tagctgagaa acatggaatt tatctagttt ctggtagtat tcctgagtta gaagatggaa
     6121 aaatctataa tacttgctat gtatttgata aaaatggagc tctaattgga aaacatcgta
     6181 aaatgcactt atttgacatt gaagttacag gaaaagttag ttttaaagaa tcagatactc
     6241 ttacagctgg aaatgatgtt actgtaatag ataccgaata tggaaaagtt ggtatagcta
     6301 tttgttatga tattcgtttc cctgagttat cacgtttaat ggctcttaaa ggtgctgaaa
     6361 tcgttatctt gcctgctgca tttaatatga ctactggtcc cgcccattgg gagttatcta
     6421 taagaatgcg tgctttagat aatcagatat tctatgttgg tgctgcacct gctagaaata
     6481 tgaatgcttc ctacatagct tttggaaatt caagaatttc agacccttgg ggaagaataa
     6541 ttgctcaagc tgatgaaaaa gaatgtatta tttatgctga cattgataga gatttaattc
     6601 ctgacataag acaacagtta ccacttctta agcacagaag aactgattta tatgaactta
     6661 atactttgaa ataaaacaga acataacccc ataaatcata aattaagtgt taaaaaaatt
     6721 tatggggttc tcattaattt gatttaaaat taatttcata aaaataatac taatattaat
     6781 ttaattaaat aatactaata attactaatg attatgctaa ttatcagcat ttactaattt
     6841 aatcactatt agttatttct tttattaaat atctttactt tttttactta ttatgtcttc
     6901 tagctttaca tttggattat agtatttctt tctattttta gtcacatctt tctattgaat
     6961 tacttgctta aaacaccaat gcacacattc cagacaatgt tcacaattat gtaaaaatct
     7021 tgctctacct tcttcaatgt tgatattatc tctcctagtt gatattatct ttcctaaaat
     7081 ttcttttaat taaaaaacaa cattcgttga tatatcgagt atattttaag caactgaaaa
     7141 aaacaataat aattgatata tcaactaatt aatgtagatt aattcatttt tataatttat
     7201 atacttttta attaatctac ctttttaatt aaaattcttc ctttctattt atccatttta
     7261 aacttaaatt ataagatttc atcagctgtg tttttgcatt
//
LOCUS       DBGJFU010000165         7122 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.168, whole genome shotgun sequence.
ACCESSION   DBGJFU010000165 DBGJFU010000000
VERSION     DBGJFU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.168"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            128..1021
                     /locus_tag="ACOIAS_003412"
     CDS             128..1021
                     /locus_tag="ACOIAS_003412"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HGZ2703080.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1199..2023
                     /locus_tag="ACOIAS_003413"
     CDS             1199..2023
                     /locus_tag="ACOIAS_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2703081.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2149..2730
                     /locus_tag="ACOIAS_003414"
     CDS             2149..2730
                     /locus_tag="ACOIAS_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2703082.1"
                     /translation="MKADSTKEQLINATIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2834..3010
                     /locus_tag="ACOIAS_003415"
     CDS             2834..3010
                     /locus_tag="ACOIAS_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703083.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4276..5028
                     /locus_tag="ACOIAS_003416"
     CDS             4276..5028
                     /locus_tag="ACOIAS_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703084.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5018..>7122
                     /locus_tag="ACOIAS_003417"
     CDS             5018..>7122
                     /locus_tag="ACOIAS_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703085.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIG"
BASE COUNT         2649 a          946 c          946 g         2581 t
ORIGIN      
        1 aaatataaaa aataggatat tattataggc aataactaac atcaagcgat aacttaatac
       61 atataaaaat aaataaacaa ttaataaaaa ttaaaacttc attataaaac ggaaggatag
      121 tgaaaaaatg caactatata acaccccctt taatacaaac aaacacttaa tctatttctt
      181 tacatttacg ctagtattga actggatatt cagtcttaca cctgcgttgc tcggaattac
      241 aaatacaata cttggagaaa tcatttttta tcttgcatat acatcaccag ctattgtagg
      301 tctatttttt atttctacaa tctatccaca aagtgcaaaa caagatttta tttatagata
      361 cctaagcttt aaagaaatta gaataaaatg gatactatta acaatattat tttttgcttt
      421 aacttttatt acttcgttaa taatcggaaa ttactttaat gtaaaaactt ctgatataga
      481 ttggatacac atagcagtgt ttagaccttc taaaatagct tatatgctat tagcaagttt
      541 tatattgagt atacttagtc aagaagctgg atggagagga tatgccattg acaaattatt
      601 agttagattt ggatttatag gctcttcaat tattttaggg cttgtttgtg gaatctggta
      661 tctagggtcg tattttacac ctaatcaaat accatataat ttggcacaat actccctatt
      721 tgatgctttt ttatttatac caaacattgt acttttaaac tttataatta attttgtata
      781 tatcaataca agtagaagca ttttatcagc tgggttagtt catatgatgt actatttttt
      841 caacattcaa ttacttctac actaccctat taaattatgt gtaatagctc aatatgtaca
      901 aataatattt ggtttaatat ttcttgtata tatgatttct tctaataaat ttaaacagaa
      961 ggtaaattct gaaattgaac agataaagtc ggatgatttt gaatttgagt tagactggta
     1021 attttaatat gaattataaa ttaagataat ctatgatttg atacgattac tacttaaata
     1081 taatttactc tctataattt attgcaacat ctggaaaata attgtatttc cacaaacgct
     1141 tgaagtatat aaaaacaaaa tatatactga ttataagtga aaaggaggtc atattttcat
     1201 gtttaaaata ggagaggttt caaaactaac acaaatttct attcgtatgt tgcgttatta
     1261 tgatgaactt ggtattttaa aacctgcaga aatagacaaa tatacaggtc atagattata
     1321 ctcagttgaa cagatttcaa tactacaaag aattgtatta ttgagagatt caaaatttag
     1381 cgttgcagaa atagcaaata tcgtacataa ttggaatgat gaatttgtaa ttaaagaatt
     1441 gaatagaaaa aagaatgaaa tacaaaagga aataaatcag gaacaacaaa gaataaataa
     1501 aattgataaa tttatagaag ctataaactg tgataaagat gaaattcatt acaatgttgt
     1561 ctttaaaaag attccaagtt ataaaatcat atcacttaga gagataattc cagactacca
     1621 atctgaaggt atactctggg aaaagctatc taaatttatc aaagaagaac atatagaagt
     1681 atcacgtcaa tccaacaata acatagcttt ttaccatgat gaagaagtca aagataatgg
     1741 tgtagatatt gaagttggaa tggttgtaaa gaagattgga aagaacaaat ctggatttat
     1801 atatcgtgag acagaagaaa ttgatatgat ggcatgtact atggtttatg gtccatatga
     1861 aaatatagct ggtgcatatg aatcgttctg ttattggtta gataaaaata gtgattatca
     1921 aatatcaggt ataaatagac aaatcggaca taaaggtgaa cataatgaaa taaatcctga
     1981 aaattatctt acagaaattc aaatccccct tataaaagtc taatagatta gtacttaaat
     2041 acttaagtac taatcaccca ttgacatccc aaaattttca tgatagtatc taaacgtgtg
     2101 ttaaacatgt ttaatttttt tatggggaaa cttttaaggg gaaaaattat gaaagcagat
     2161 tcaacaaagg aacaattaat taatgccaca atccagttac tatcttcaca tagagatgtt
     2221 tcaagcatta cggctagaga aattgtagct aaagcaaatg tcaatttagc tatgattaat
     2281 tattatttta aatctaaaga ggagttaatt aatatttcta ttaaaaaatt tttaaagcgt
     2341 catatggatg aatataaggc tacacatgat actaaaaatt tagcacctaa acagcaatta
     2401 agggaaatac taattgcatt ttttgatttt ataggtgaac atagtcaatt tataaagatt
     2461 tctgtaccct atatattgat acaggaagaa attgtttatc catttgagat actaccttta
     2521 attaaggctc actatgaaaa tcagaataac aaaaaaactg aagctgaata cagaactatt
     2581 gcgtatcaaa taacttcatt tattcagttg ctttttcttc gttctaatgc ttttttcaag
     2641 tatagtggta ttgatattct taatattgaa cagagaaatg gtttaataga ttttcaatta
     2701 gatttattat tggtagatat tacaaaataa tactatatta attttttatg tacatttttt
     2761 tattttatgg taacgtttga aaatattctc attgactaaa aatacttgca ttaaataagg
     2821 gggaggaatt ttaatgtttt ttacatttat aggagtaata tcagcaattt tattgttgtg
     2881 tacaattata tgtggtttat ggataaaact taataatcgt aataatgact ctaattctca
     2941 ttttactttt tcaatacttt caattgcttt ttcactgtta attgtggtgg cctttttagt
     3001 aagaaattaa tgtctttgtt atgctgaggt ttattattat agatttttat tattgcatga
     3061 tttttactat attatagtat ttttattata ttacagggtc ttatttatta tagtgttttg
     3121 ttgttacagc attttattat attacagagt cctatttatt ataggatttt tattatattg
     3181 taaaatctta tttattatag gattttgtta tattacagac ttttattata ttgaaatctc
     3241 ttatttattg cagtgtttct attatattgc aaactcttat ttattatggt gttttgttat
     3301 tgcaacattt ctattatatt gtaaagtctt atttattaca gttctttatt atactacaga
     3361 cctttattat tacagcattt ctattacatt gtaaagtctt atttattaca gttctttatt
     3421 acactacaga cttttattat tacaatctct tatttattac agttctttgc tatactacag
     3481 acctttatta ttacagcatt tctattacat tgcaaagtct tatttattac agttctttat
     3541 tatactacag acttttatta ttacagcatt tctattacat tgcaaagtct tatttattac
     3601 agttctttat tatactacaa acctttatta ttacaacatt ttattatttt aacttcttta
     3661 ctataatata gtaatatttt aatatctcaa aatcatatca atcacttttc agcatagtct
     3721 aaaatacttc cactttctaa aattatctat catacctata ctaataatac tttttaacct
     3781 ttttattata catttcaatt gtttctaaca acctatttat ttccaaaact ccaatatatt
     3841 ttctcaatta cattgtgtta atagccccta aatcctttca aattatttac aatacattct
     3901 attaaataat cttactgctc tataattctt tttttctttt taaccctact atacattatt
     3961 tacatcattt ttttcaatac aacattcgtt attctataac ctttttactt ttactatata
     4021 cttcttttct aactacacct tcaatgttcc acatcatatt actttagcac aacaattaat
     4081 acctaaaaat aaccctctaa atcctccaat aatataatca atttaactat aataataacc
     4141 tctcacacta tccaaacaat atattttcaa acattacaaa aaaacatcca actattacaa
     4201 tatagctcat atccctcctc ccctttcaaa tgctatcatt gacttacaag ataaatgaaa
     4261 gtgaggatga tatatatgct taaagtaaac caattatgta aatcatataa aacaggcagt
     4321 aaaacatatc ctgtattaaa aaatatttct tttgaaatca acaaaggaga atttgtcgca
     4381 atcatgggtt catcaggaag tggaaaaact actcttttaa attgtatttc atgttttatc
     4441 ccaattgata atggggaaat aattcttgga aataataata taagaaattt aaaagaaaag
     4501 gaactttcag aagttagaaa tcaacaacta ggatttgtat tccaagactt catgctattt
     4561 gatggtctta gtgtctttga aaatatatgt gttccacaaa ttatcaaagg tagtcaagtc
     4621 ttacaaatgg aaaaccgtgc aattaatcta tgtgagctat ttggtattga acatatcaga
     4681 aaaaaatacc ctgccgaaat atctggtgga gaaaaacagc gtacagcagt agcaagagca
     4741 ctcatgaaca acccagattt aatattagca gatgaaccaa ctggtaacct tgatagtaag
     4801 tcttgtcgta cagtcattga atcattttta aaagcaaagc aagaattaga tgctactata
     4861 ctcatggtta cacacgatag ctttgcagca agcttctgtg accgagttat tgtgcttaaa
     4921 gatggaataa tttataaaga agttgtaaaa tcaaattcaa gacataaatt cctagatgaa
     4981 ttactagaag ttttaaaaga aatgggaggc gctcaagatg acaattaata aattatacag
     5041 taaacttcga gcctataatt ggaagaacta tacaacttta ttcttttgta taatactttc
     5101 tgtggtatta gtaacttcat atgctctaat ttattttagc ccaacaatac aaaacatact
     5161 tccaactggt ggagatagca gaaaacaggc tacaatgata ttctcagttg ctatattagg
     5221 atgcgctgtc tttactacat acgcttcttc cctcttttta aaatttaaaa gcaaggaaat
     5281 tggtattttc atggctctag gaacagataa gaagaaatta aaaaaacttc tattttcaga
     5341 gatattgtta atttcaatag tatgctgttt tttaggtctt atattaagta ttcctgtatc
     5401 ttttggtata tggaaaatat ttcaattagt gattattgat accagagaaa tgacatatca
     5461 ttttggaact caaggattca tatatggatt tatatttagt gtatttgtga cattatgcat
     5521 atttatactt tctgtaaaat ttattaatcg tacaaatata attgatatat taaatgacca
     5581 gagaatatct gaaccaatac aggatgtaaa ggactggtat ggtatattag gtataatttt
     5641 tattatactc ggtatttttc taggatatct cgtaccttca ctaactataa acaaattaaa
     5701 ttataaacta ccatctatat ggagctttac ttatctactc tctcttattg gaatatatat
     5761 gattatgaca tacatagttg tacatgctaa aagaggaaaa catccagaga aatactataa
     5821 aaacatcata tcaactagca tgatgagatt tatgggaaga caaacagtaa aaaacatgtg
     5881 tgttatttca tttttgattg caggagcatt atttgctgta ttctatgttc ctaatatggt
     5941 tacaggtatc tatgatactc ttaaaaataa ccctatagat tatactttta cctacaatca
     6001 aagagataaa caggtatcaa aaaaagatat tgaacaatta gcagataaac acaatataaa
     6061 aattcaaaac tatatggaaa ttccttctat agaattaatt gttgatggtg tagaatcaat
     6121 atatcataaa gatggaacta tcgaaaaggt atatatgaaa aaaagctcct atgatatgtt
     6181 ctttaattct aaagattttt ccaagctaat tggcaaacca ataactataa aaaatggtga
     6241 atatcttgct gttgtaaata aaggaaatga aaatcaagat tataaatata ccaaatttat
     6301 atcaagccca gtcacagaaa aagaaatccc ctttagatat gctggaaaaa taatatttaa
     6361 tggttctttt tttcaaaata gaagtatgaa cgcctatgtc cttaatgatg aggattataa
     6421 atcatttact aaagatttat ccataaaaaa ttacaataat tctatactat ttaatgtaga
     6481 taaagatacc tatgcttttg ccaatgactt aaagaaagaa attataaaaa gaacatctaa
     6541 ggaaatgggc atgttcagtg gttatgatga atacgtaaaa aaacgttatg aatcaactgg
     6601 aagaaaatat tttatggatg aagaatatcc aagtggtgaa ggtcatttaa aactagaccc
     6661 aaacaacaat cagttgtatc ttaattggaa atactatcct aactttaaaa ttttacaaag
     6721 ccaagatatg ataaaaaaca tggcagtctt cttaatgtta ttcatatatg tggcaatcat
     6781 atgttttaca gcagtaggaa tcattgcata tacaaggggt gttactatag ctataaatca
     6841 caagcaggtc tttatggatt taaatcgatt aggagcaaac aatgattata taaagttctg
     6901 tattagaaat caattaaaga aagtattttc ataccctctt attgcaggaa gttttttaat
     6961 ttatctgttt aattttatga ttatgttaaa taatgatggt agaatcgtca atagtgaggt
     7021 caatgctctt ttgataaact taggttttat agggatttgt ggggtttata tgtaccttat
     7081 atatctatta acttataaga aattcaaaaa aattattggt at
//
LOCUS       DBGJFU010000166         6760 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.170, whole genome shotgun sequence.
ACCESSION   DBGJFU010000166 DBGJFU010000000
VERSION     DBGJFU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.170"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..1816)
                     /gene="recJ"
                     /locus_tag="ACOIAS_003418"
     CDS             complement(<1..1816)
                     /gene="recJ"
                     /locus_tag="ACOIAS_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGZ2703086.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTD"
     gene            complement(1829..3493)
                     /locus_tag="ACOIAS_003419"
     CDS             complement(1829..3493)
                     /locus_tag="ACOIAS_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HGZ2703087.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(3791..4288)
                     /locus_tag="ACOIAS_003420"
     CDS             complement(3791..4288)
                     /locus_tag="ACOIAS_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGZ2703088.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(4317..5696)
                     /gene="scfB"
                     /locus_tag="ACOIAS_003421"
     CDS             complement(4317..5696)
                     /gene="scfB"
                     /locus_tag="ACOIAS_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HGZ2703089.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(6001..6147)
                     /locus_tag="ACOIAS_003422"
     CDS             complement(6001..6147)
                     /locus_tag="ACOIAS_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HGZ2703090.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(6177..>6760)
                     /locus_tag="ACOIAS_003423"
     CDS             complement(6177..>6760)
                     /locus_tag="ACOIAS_003423"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HGZ2703091.1"
                     /translation="KMFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITT
                     MLFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENIN
                     IDLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILS
                     LSLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
BASE COUNT         2189 a         1243 c          636 g         2692 t
ORIGIN      
        1 tatctgtatt tccatcaaat aattcaaaat cagacacaga tatattatac aggctttgag
       61 tatcttttgg tattattttt gacattaatt tcaatgaaag tttgtcatta cttgcacttt
      121 taggtcgagc caatcgtata tctttaagta aaaattgtac tttcctattt cccatatagt
      181 tattttcatc taattggaat agtacatcta ccttgtctcc tactttatac attgatttta
      241 aatgactcat attaaatcct atacattcat agcttttctc tttttctatg cttaatttca
      301 gatgttgttg attcttacct ataactttca aatcttttaa aatataattt ctaactataa
      361 atctaggatt aggattattt aatccaaagg gttctaactt atgtaattct tctacaagat
      421 ttaaatttat tacattttct tgtaattcaa actcaacatt tacattctct atcatatcat
      481 cttcagttaa attataatct gcaaatttat ttatttcatt tgctaaaatt tctacattgt
      541 ctgaatccaa ggataaccct gctgcttgtt catgaccacc aaacttagtc atcaagtctt
      601 tgcactttat gagagcttca aatatattaa atccttttat tgacctcgct gaaccagtag
      661 cctccccatt ttctatacct agaagaattg ttggtttgta gtatttttca gttaactttg
      721 atgctacaat tcctattatt ccatgttgcc aaccctcttt agctaaaacc aacaccttgt
      781 catcattgta tctgctatta gactttagca tttcttcagc ttcatgatac atcttagctt
      841 ctatcatctg tctttcatta tttttttctt ccaatatact agctatttct actgcctctt
      901 cttgtgattg agtagtaaat agctctactc ctaagtaaga atatcccaat cgacctgaag
      961 catttatcct aggacctatt gcaaaaccta tatgagaaga acctatctta tcactttcta
     1021 caccacaaac ttttataagc tcccttaatc caatattttt gccttctttc attgatttta
     1081 aaccattttt tacaattatt ctattttcat ctataagtgg aacaatatca caaatagttg
     1141 ctagtgttac tatttccaaa taattataca tactagtttt aaattcttct tttggagtca
     1201 aggcttgaat cattttaaat gctacaccac atccacatag tgaatcaaat ggataattac
     1261 aatcttcttg ttttggattt attactgcaa aggctgatgg aatttcactt tgacattcat
     1321 gatggtctgt aattataaca tctatgccca aatcattcgc tgtattgacc tcagacactg
     1381 atgtaattcc acagtcaaca cttataatca agtcacatcc cctagagctt ataagcttaa
     1441 tagcatcttc atttatacca tacccttctt ctaatctatt aggaatatag taatcaacat
     1501 tgtaacctat actcttaaaa tataagcaca agatagatgt agatgataca ccatcaacat
     1561 cataatctcc gtaaatccaa attttttcgt tcttctcaac tgctaacttt attctatcaa
     1621 cagctttttg catatctttc attaaaaaag ggtctcttaa ataatctaaa gatggattca
     1681 taaatatttc tgaatctttt tcagtacata tccctctatt ttttaaaatt tggcagattt
     1741 ccggggaaat attaagtttt ttgctaaatt cactttcctc tatcttacct ttatacttca
     1801 atatccattt tttcaaattt aattccccct aatctttttt gtattttgac ctaatacttc
     1861 ttataactaa gcaaaggcct attataaagg atataaagaa acccgctatt gcagtaaatt
     1921 ttatccatat attattttct atatttggag atgcaattat aagagaagaa cctactataa
     1981 tagatgccaa cactaaactc aaagatattt gagatgttag ctctattata caattttcta
     2041 acctcgtcat tttaacatct tcaatttgca tttttatatt atttctttct atatttttta
     2101 aaattgcttt taattgcttt ggaatagttt ttatgtccag taatatttct tctacatttt
     2161 gtcttgaact taaaacaaca tttttagggt taaatttact cttataatga tgtttcataa
     2221 actcttttcc catagaacca aaagaaaaat ctgtgtttaa agttctactt gttccttcta
     2281 atgttataac tgtctttgca agagttacaa attgtgcagg catgactatt ttatactgtc
     2341 taaaaaacct aaatagttca tttaatatat ctgttacatt tattttttct atagaaatat
     2401 catagtaata atgtattaga taaagtaaat cctgtctaag tcccttaata tcagcctctc
     2461 catttaatgc atccatttct atcaatagat atattatctt gtcaacattc ttttctacac
     2521 ctgctaatgc aatttcattt aattggttta aagttttatt atcaataatt ccaatcattc
     2581 caaaatctat atatgctatc ttatttttag caactacgaa tatattacct ggatgtgggt
     2641 ctgcatgaaa aaatccatga gataatacct gtgtaaaaaa tgacctaact cctatttcaa
     2701 caatagtttt tgtgttataa cctagtctcc ttattttttc tacatcactt aactttgtac
     2761 cattaacttt ctccattact aaaattttct tagtattata ttctgaatat actttaggta
     2821 tatatacttc atcactattt ttaaatatcc tagagaattt agttgcattt atagcttcaa
     2881 atgtataatc aagttccctc attaattgag tttgaaattc ttcaatcatc tgtactaaat
     2941 ctaaattaaa atcctttttt aaatctttta aagtatttgc aatagttcta agaatctcta
     3001 aatcagattt tattattttc tctatatttg gtctttgaat ttttacgatt acttcttctc
     3061 ccgttttaag tacaccttca taaacttgac caattgaagc tgctgctatt ggttcttctt
     3121 taaattctaa gaatatttct tctatactta aacccacttc ttctttaaat atatcaatag
     3181 ctatatttga atcaaatttc tctacatcat ctctgagttt tgatatttca tcaattatat
     3241 cctggtctaa caagtctttt cttgtactca atatctgtcc tatttttata tatgtaggtc
     3301 ctagttcttc taaaactctt ttcattctct caccagttgt catatttttt atttcttctg
     3361 gtgggtcaaa tagtggtatt ttataggcaa ttccttctat atttagtctt tctacaataa
     3421 aactaaaacc atactttatt aaaacatatc caatttctct atatcgcttt aaatttctat
     3481 aacttattct caaatctttt cactcctaag ataggtatac ccaaaaactt acaccttaat
     3541 ttatcgtata tgacattatt tcaccaattg atattctaat atcctatatc ctctttaata
     3601 tttaatttta tcacatatac taccaatttt attaaaatta tttaactcag ttaattttta
     3661 tttttgacat tttatatctg gtttaatcaa atactttttg aaaactaaaa tatcaacaaa
     3721 atctaataaa ataaattagt ggttgcctca aaacagattt aaatatgttt tgaaacaacc
     3781 actctttaaa ctaatatttt tgattttgtg ctgcctctaa ataccattca taagattcat
     3841 caatatattt tttatcttct tctgtaagct ctctaacaac cttaccagga gaacccatta
     3901 tcaaaacacc tggtggaaac ttcttattag atgtaattag tgtacctgct cctatcagag
     3961 tatattctcc tatttcagca ttatccaata ttattgaacc cataccaatt aacacattat
     4021 cactaatttt acaaccatga actatagacc tatgcccaac tgttacattg ttaccaatta
     4081 tagtttctgt atctccatgc actatagaac aatcttgtat attagtgttt tctccaatag
     4141 ttattggtcc ttcatcaccc ctaactactg cattgtacca tatacttgaa tctttgccta
     4201 ttttaacatt tccaattaca tctgctgatt ttgccacaaa tacactttcg tcaattaaag
     4261 gtttatcttc taaataatct ctaatcatat ataaacaact cctttaaaat ataaatttag
     4321 ttggttgctc cttctaacat taacttagct tgtatcatta tagagcactc tgtacgtttt
     4381 ctttgcattt cacatcctaa ttcatatggt tttgatatgt cattattaaa gtttatcgca
     4441 tttgcatgac atccacctga acagtaaaac ttattccaac attgtttaca ctcttcttta
     4501 gtatatacat gagcttctct aaacatattt tttaaatttt ctggaagtac tatttcttca
     4561 tcaaatatat ttgccatttt aaattcttca tttccaacaa attgatgaca tggatatata
     4621 tctccatttg gagttacaga aagatactca tttccagctc cacatccagt tattctcttt
     4681 attacacatg gaccttgatt taggtctatc ataaaatgga aaaatttaaa tccatctcct
     4741 tgtaattgtc tatctgcata ctctacagca aatttttcgt actcttcaaa tatcttaggt
     4801 aaatcttctt ctctaagtgc atatggatta ctctcatctc caacaactgg ctctacagag
     4861 gttaatttaa atcctaaatc tgcaaaatgc ataacatctt ttgaaaaatc taaattatct
     4921 cttgtaaaag tacctcttat ataataatac ttatcttttg ctcttttttc cacaagtttc
     4981 ttaaatcgtg gtaatgtaat atcataactt cctttatcat ttaaagttgg tctcatatta
     5041 tcattaactt cttttcttcc atctaaactt aaaactacat tatgcatatt ttcatttata
     5101 taatctataa tctcatcatt tagcagtaca ccatttgtag ttattgtaaa tcttatattt
     5161 ttattataat ctttttctac acttcttcca tagtcaacca gttgttttac aacctcaaag
     5221 ttcatcaaag gctctccacc aaagaaatct atctctaggt tctttctact tcctgaatta
     5281 gcaatcaaat agtcaattgc tgccttacca acttcaaaac tcataagttc tttttctcct
     5341 ccaaaatcac cttgagctgc aaaacagtat ttacatttta agttacaatc atgagctaca
     5401 tttaaacata aagcttttac tacaggctct ctatgtacaa agcttggatg atattgatat
     5461 gtatcttctg tatataaaag accctctttt tccaaattta atatttcttc atatgcttca
     5521 tttatttttt cttcttgata ttttgatttt aatatctcta atatttcttc tttacttttt
     5581 tctttataaa aatctaacaa atcgtatgca acatcatcta acacatgaac agctccacca
     5641 ttcacatcta aaacgatatt atatccattc atagaaaatt tatgtatcat actcataccc
     5701 tacctcctaa attactatct atactaaagt tttttattat tttaattaaa ctttaccctt
     5761 cattatatca taagtttaca taaaatacat acaatacaaa aatgacttac tctccgtaag
     5821 tcatttcatt aaatacacta tatcccccta aattggcgca ctaatatatt accaaatagg
     5881 tattaggctc ctataaaata aataaaataa attttgtatc tgattttata ttgttttaat
     5941 cttattaaaa tataataatt tcttttttcc tgataatttt gtataaataa gtgcttgtat
     6001 ttaatttgtc ttctttagat tgctgatttc tttgggtact ttaggctgat gaataatcca
     6061 aggacaggta ctattagctg acaaaatagc agtgctaagt gccaatgagc tagcaaactt
     6121 cataaatctt acaataaact ttttcataac ttaactctcc ctcatacata ttttacttat
     6181 actttagctt ttaacttacc taatactaac atcacaaaaa tccaaaacat ggcaaatgcg
     6241 ctatatgtga aataatctac aaatatactc aaagaaagac ttaaaattgt tatcaataac
     6301 actactgtag atataaatat agcagttttc ttataaacca atttttccct atcacttata
     6361 gggttactcc tatgttctat tgggcacaga acacaaattc caacccaact aacacttgca
     6421 ataatcataa taatattttt aaataagtct atatttatat tttctataat taagattgta
     6481 gataaaatta taaaaataaa ggttaataag catctaaaat aattgtctgc atgataccct
     6541 cctgaaaatt gtcttattgg acaataacac attaaaaaaa ataatacata tgtaaattta
     6601 ccaaataaaa aacctataaa cagcatggtg gttatattaa ctatgaaagc tattaatatt
     6661 tcaaatccat aagaatagac tttagattca ttattatcta tcatattatt acaaattaaa
     6721 acagatgtca ttttttcagc atatctttta aacattttta
//
LOCUS       DBGJFU010000167         6584 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.171, whole genome shotgun sequence.
ACCESSION   DBGJFU010000167 DBGJFU010000000
VERSION     DBGJFU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.171"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(619..1179)
                     /locus_tag="ACOIAS_003424"
     CDS             complement(619..1179)
                     /locus_tag="ACOIAS_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HGZ2703092.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="ACOIAS_003425"
     CDS             complement(1225..2208)
                     /locus_tag="ACOIAS_003425"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HGZ2703093.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="ACOIAS_003426"
     CDS             complement(2221..2583)
                     /locus_tag="ACOIAS_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2703094.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3826..4473
                     /locus_tag="ACOIAS_003427"
     CDS             3826..4473
                     /locus_tag="ACOIAS_003427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703095.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4748..6385
                     /gene="repA"
                     /locus_tag="ACOIAS_003428"
     CDS             4748..6385
                     /gene="repA"
                     /locus_tag="ACOIAS_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HGZ2703096.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2683 a          692 c          917 g         2292 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taaacttatt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaaa ttttcgtctg
     3121 catgtaaaaa tagctatagt aatgcgtacg aatgttctat tattacagaa ttatttgtat
     3181 gtaaattata ttgaaaatat ctatcagaat tagaatttta tatggataaa acagaactat
     3241 caaataggag tatatagtaa agatttatat aatatgatga cctcgtacaa gcctgtaaga
     3301 gccgtttaaa agttgcttta gtattaatta tctttgaaaa tttaaaatag gtttaaatgg
     3361 cttgttaaat atgatgtatt actattatgt ataggggggt agttatgtag ttacatgttg
     3421 ttttaaaaaa taaatttaac tatagttctt tcacttccac attacaaaaa ataaattgag
     3481 tgtgtatgtt tttaagtaaa aatgatgttt aaatattgaa aaatagagat atagtgtatg
     3541 taaaatttag aatagtgtat gtaattttag gcataaaaat atgtgattaa accttaaaaa
     3601 ccaggaatat agtgtatgca tttttaaaaa taaaaattgt aacctttttg taatctttta
     3661 ttaaaatact tactttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     3721 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     3781 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     3841 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     3901 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     3961 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     4021 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     4081 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     4141 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     4201 gattctataa atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     4261 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     4321 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     4381 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     4441 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     4501 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     4561 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     4621 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     4681 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     4741 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     4801 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     4861 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     4921 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     4981 ttatgaaaat acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa
     5041 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     5101 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     5161 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     5221 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     5281 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     5341 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     5401 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     5461 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     5521 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     5581 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     5641 atatcaagtc aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg
     5701 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     5761 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     5821 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     5881 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     5941 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     6001 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     6061 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     6121 agtaatttgg ggaataattt caactgaaga attaagatta tatactcata tgagatataa
     6181 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     6241 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     6301 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     6361 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     6421 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     6481 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     6541 taaaataatt gtacatatat atgtagtata tatatatatg tttt
//
LOCUS       DBGJFU010000168         6582 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.172, whole genome shotgun sequence.
ACCESSION   DBGJFU010000168 DBGJFU010000000
VERSION     DBGJFU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.172"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            424..1698
                     /locus_tag="ACOIAS_003429"
     CDS             424..1698
                     /locus_tag="ACOIAS_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HGZ2703097.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1905..2222)
                     /gene="trxA"
                     /locus_tag="ACOIAS_003430"
     CDS             complement(1905..2222)
                     /gene="trxA"
                     /locus_tag="ACOIAS_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGZ2703098.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2489..3241
                     /locus_tag="ACOIAS_003431"
     CDS             2489..3241
                     /locus_tag="ACOIAS_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGZ2703099.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3228..4109
                     /locus_tag="ACOIAS_003432"
     CDS             3228..4109
                     /locus_tag="ACOIAS_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HGZ2703100.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
     gene            complement(4335..5690)
                     /locus_tag="ACOIAS_003433"
     CDS             complement(4335..5690)
                     /locus_tag="ACOIAS_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HGZ2703101.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2532 a          946 c          827 g         2277 t
ORIGIN      
        1 cttgtacatc ttttataaca tataaaatct ttttttctaa ttatatacaa tatattttcc
       61 attatgttct aatcaatttc tttactattt ttacaacttt attagtcata ttcataatta
      121 tttctaatta gtgtttaaca ttttatgtaa aatttataaa aaaatgtata taaattaaaa
      181 taagtgacta tactaaaaat atgatacgga taaacaataa gttcaaccta tctcccccaa
      241 aatcaagtga gctacagttt cccccaactg tagctcattt tcgtgtaatt taataaacat
      301 ctcaacaaga attaattaac aaaacccctt ataaaaacat aacatatatt aaacaagctc
      361 ttgattaaaa cttgagcttt tttaataaaa aacagaatag tttattagat aaggggtttt
      421 tttatgcaat tcatggatgt atctaaatac attgaaggtg caaaccttaa agttaaaact
      481 aaaaatggtg aaactgttaa aagaatatat tttaacaact ctgccactcc attagtttta
      541 aaaaacgtag tggataactt aaactgtgag attccttggc ttacttatat taatgctcca
      601 ggaataatct cagaaaaaaa tactttaaaa tatgaaaatg tacgctctac tatcctcaaa
      661 cttattgatg gagacgaaga aaaggattct gttatttatg tcaaaaatgc tacagaagga
      721 ataaacttct tagcaaaatt ttttaaagat gaaaatccag ataaatttgt tattaccaca
      781 gcaatggagc atatggctaa ttacctgcct tttaaagtta attttcctac aaaagttgtt
      841 ggaattacag aaaaaggaga acttgattta tgtcaattag agaatatact taaaaattat
      901 gctggtaatg tatctctcgt tacagttaca ggagctagta atgtcacagg tataaccact
      961 cctatatatg aaatagcaag aatagctcat aagtatggtg caaagatatt ggtggatatt
     1021 gtgcaagtaa ttcagcataa acctttttca atgatgccac atgaatgtga tgagcatata
     1081 gattttgttg tttttagtgc tcataaatgt tattctccac taaatggagg agctctggta
     1141 ggacctaaaa aattctttga aaaatttaat cctgtactat atggctcagg ttctaccaaa
     1201 tttgtaaatg ataacaaaat actatatgca aacgtacccc aacgttttga agcaggatac
     1261 ccagagtatt ttggaactct tgcaatggga aaagcactga acactttaaa taaaattggt
     1321 ctaaacagaa tttcaaaata cgaaagagaa ttatttttat atgctaaaga agaaatgagt
     1381 aaaataccta atataataat atatggagat aaatcaaata atgtaattat cccaaatata
     1441 tcttttaata taagtaatat gtattacaaa gatgtagcaa aatatttagt tgataacttt
     1501 ggaatagaaa ctggagctgg agtagtaggt gcagatattt atgtacaaaa attattaggt
     1561 ataaatccaa aagaggcata cgtcagattt atgagggaaa atcctgtagg tcttgttagg
     1621 ataagcttag gaatgtacaa tacttttgat gaggtggata gctttataca tgctattaaa
     1681 actttggctt ttaaataaga tttttctatt cttttttatc aataaataga gagtagtttt
     1741 gttttcacaa aaaaacaaat tatatcaaca gtcaatttca ttagcaatta gacactcatt
     1801 tgtaactata caaactattt tacactatac aaacaaaaaa ctcattacaa tgagtttttt
     1861 tgtttatata tgagatttat tttaattact tcaatttact ttaattacat atatgattca
     1921 attttgtttt ttaaattctc cttaggcata aaacctatta atctatctac aggttttcca
     1981 tccttaaata ttagcatagt tggtactgta gatacttcaa atttctgagc taattctata
     2041 ctttggtcta tgtccacctt caagaagttt gctttttcaa ccatctcatt tcctaaagct
     2101 tcatatattg gtgatagcat cttacaaggt ccacaccaag tcgcaaagaa atctacaact
     2161 actaaaccat ctttattttc tacttcattt ataaattcat tattatttat tatttttacc
     2221 ataatataca tctcctttat tatcttcttg atattaatat actacgccct atataacagt
     2281 tcaattaggc acttttttgt aaccgcctat ttttaataga tttaatatta tgataaatag
     2341 atttataaga ttaaatattt atgagactaa atatttctaa tatattttat ttttttctta
     2401 atatagttga cattttttcc cttaaattat aaaatcaaat taataactac ataggtagtt
     2461 actaatcttt tatcaaagga gtttctatat ggataatata attaatgaat tacaaaatat
     2521 aggatttact aaatacgaat ctcaaatata catatcatta ttaaaggaaa gccctttaac
     2581 aggatatgaa ataagtaaat tatcaggtgt accacaatca aaagtctatg aaaatataac
     2641 aaagctatta aacaataata taattatcaa tgttggaact gaccctatta agtacatacc
     2701 aataaaccct aatgaattat taaaaaataa gaaaaaagaa tttgacaatt ctatggataa
     2761 tctaaagaat agcttacaga tattaaataa aagtaaaagt gtagaatacg tattaaacat
     2821 aaaaggagat aaaaatatat taaaaaaagc aatagaaatg ataaaaaatg caaaacaaga
     2881 aattataatt tcttgccctt atgaagaaat tcttgaatta aaaaaagaac tcctaattgc
     2941 ttacaaaaaa aatattaata ttgaaatact acttttagaa aacaaaagtc tgaaaggtct
     3001 tgataatatt catattcatg gaggtaataa aactacactt gaaaataaaa tgctttacaa
     3061 aggaattatt ttgattgtag ataatgatga aatattgaca ggaaacttat cagaaggcag
     3121 tgaggccatt tcaatttgga caaaaaactc taacatttta tatattggag ttcaatacat
     3181 aaaacatgaa atttatattt cagaacaaat aaaaggaggt tataaaaatg aacaaaatta
     3241 agattaaaca agttgatgct tttacaacta taccttttgg tggaaatcct gctggtgtcg
     3301 ttactgatgc ctctaacatt tctgatgaaa ttaagcagaa tgtggctaaa gaaatgaatt
     3361 tatcagaaac tgcatttgtt tcagagtcaa atattgcaga ttttaaagta caatttttca
     3421 caccaggttt agaagttgat ttatgtggtc atgcaacaat aggtactttc catgcacttt
     3481 tggaagaagg gaagcttaat actaataaga gtatatttta tcaagaaaca aaagctggtg
     3541 tattatctgt agagcttaag aaaataaata atgaaaatat atttatgatg gaacagaata
     3601 cacctgaatt tgaaaattta gataattgca gaaaagaact tgcacaaatg ataggtgtgc
     3661 atgaagatag acttcttgaa tatcctatta taaaggtaaa tactggactt tggtggcttg
     3721 tatttggtct aaaatcttta gatgatttaa aaaatatctc tccagattta aataaaatca
     3781 aaaaatttag tggtgataat aatataattg gtataactcc attttgtata gaaactatag
     3841 ataaaaagtg tgactaccat ttaagatcta tagcaccata tgttggagtt acagaagacc
     3901 ctgtatgtgg aactgggaac gcttgcgtat catcttatat agtacatcac aatattttag
     3961 ataaaactag ttttattggt gagcaaggta actttataaa cagacctgga aaggtatatg
     4021 taaatataac tagagaaaac aatgacataa cttctgtaaa aattggagga agtgcttata
     4081 ctattttaag tggtgaaatt atgtattaat cccataccta ataaaaacaa ataaaaatac
     4141 tagctaacta aagctagtat ttttatttgt tatttttatt taataatcat actctaacaa
     4201 atattataat tatatatatc ataatattgt atttagaagt aaaattttat ttttattatt
     4261 aattagttat aaatcaatct aatacaacat aattacagta gattaaccta taaaacataa
     4321 taattgctta ctaattattt atcatcctca acaagtctac ttaatgtctt atcagataag
     4381 aataataata tcgctgcaac aaatagtatt actggaatta tagtatatac tttaatcata
     4441 tcaaatttaa gagtaaatgc atatatatat ccgtatcctt taccagctat aaaagtagca
     4501 aatgtccata ctcccataag aacacctaga agcttcttag gtgcatactt acttacaaaa
     4561 gagttaccaa gtggtgagaa taacatttca ccaagactta aaagaatacc aaacatgatt
     4621 atccatagta tactagcttt agcagtttca ggagctccaa cccctcttga aaattctgca
     4681 ccaacaagca ttaagaatgc aagacctaag aatattaatc caagacctgt ctttttaaat
     4741 aaactcatat ctccttgtgg tctactagca agtttaaacc ataatgctcc aagaactggt
     4801 cctaatacta tacatacaag tgaatttaat gagtcaaacc aagcaagagg aacatcaaat
     4861 cctccaacat tcatgtttac aaatgctgca ccataatcat aaactgcaag ataagttaga
     4921 taccagaata cccagaaaat tacagagaag aatgatataa gtataatagc ccaaattctc
     4981 ttcttatcca tgcttgtaag aggagactta tctttttctt caattttttc agcaactaca
     5041 ccttctttaa aaggtctctt tcctgcattc ccaaggaatc tccatccata tatgaaccat
     5101 attgcaccta ttacacaaag tacagctgct ataaagaaac attgagaaaa tccaagtaca
     5161 ccattttttg caaatgtttt aaggtaaaga ataccaactg ctatagtacc aataaaagaa
     5221 cctatattaa caaaagaata ctgtactgaa aatgctgtat caagttcttc ttgactatca
     5281 aaaagctgtc catttaccgc agatacgtta cctttaaaga atgcagtacc aattgaaact
     5341 aagattatca ttgcatttac catagaaact gtagttgcaa tagaaccaag atagtatcct
     5401 actcccatga taagcatacc tataggtata gtatatctag ctcctatcca tctatctgat
     5461 atatatcccc caattatagg tgcaaggtat gtgaatgcaa caaggttggc ttgcattata
     5521 gcagcttgtc ctttatctat tccaagacca ccatgaacta tagctgttgt aaggaatata
     5581 tatattaagt actttgaagc ataatatgct gctctttcaa atgagaaagt tgttgagcag
     5641 atataaaatc ccataggaaa tttacccttc ttttttactg ttgagctcat aattttaaat
     5701 cctttcttaa ttgtattttt attttttacg ttttgtccaa attctttctt ttatgaaaga
     5761 tataatatta ttattttgta aaacaatttg taattattat taagataatt gcgcatttat
     5821 ttgtagagta catattctca tggaataaaa ttaatacact tcttaatcat aaatttaaat
     5881 tatataaatt cactattttc agatatagtt ataaattaat atattctcat ctaataccat
     5941 gaaaaaacct tcgccatatg caattcttat tttttacgtg atagtatttt acaaactaaa
     6001 atcccccttt tgtagcaact tatgtaatta cattttagtg cttttttaaa ggagtctcga
     6061 ataaagcaac atatattttg ttcaaataat gtttttattt attttttaaa tttatatatt
     6121 acttcactca accaattctc aacttaaata actaaactac tatctattca cagattaggc
     6181 ttatttctta cacattagtt tataataaac actgtgaatc taaacataaa aactgttatt
     6241 ttaaagtaat tacccatata ttcgctttga tactatttat tgaggttata ctctttatgt
     6301 ataatatatt atttataaaa tacctcaaaa tatattattc ataaaatacc tcaaactgaa
     6361 aactttttca cgaatattta tttttcacct cccctttttt atttcaccat gtaaatcgta
     6421 ccataaatat gcacttatat gcaagaaaaa aatctattaa tcgacagtaa ataattagaa
     6481 aaattcaaat tatatttatc aagtatatta tataaaatat ttcctatgaa aatataaatt
     6541 aaattaatgt tcagaattgt ttttagatat gaaatcttga tt
//
LOCUS       DBGJFU010000169         6517 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.173, whole genome shotgun sequence.
ACCESSION   DBGJFU010000169 DBGJFU010000000
VERSION     DBGJFU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.173"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..665)
                     /locus_tag="ACOIAS_003434"
     CDS             complement(<1..665)
                     /locus_tag="ACOIAS_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HGZ2703102.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKV"
     gene            957..2621
                     /locus_tag="ACOIAS_003435"
     CDS             957..2621
                     /locus_tag="ACOIAS_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HGZ2703103.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2728..3627)
                     /locus_tag="ACOIAS_003436"
     CDS             complement(2728..3627)
                     /locus_tag="ACOIAS_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HGZ2703104.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3654..4292)
                     /locus_tag="ACOIAS_003437"
     CDS             complement(3654..4292)
                     /locus_tag="ACOIAS_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HGZ2703105.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4629..5627)
                     /locus_tag="ACOIAS_003438"
     CDS             complement(4629..5627)
                     /locus_tag="ACOIAS_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HGZ2703106.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KKISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5725..6300)
                     /locus_tag="ACOIAS_003439"
     CDS             complement(5725..6300)
                     /locus_tag="ACOIAS_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703107.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         2213 a         1036 c          718 g         2550 t
ORIGIN      
        1 tgaacctttc cagagtcctt taaatatcct aatgccccat ttatttgtga ttttaaatct
       61 atttctactt ccatatccaa atcaacattt ccttttttta tagatatatt tttaagtgtt
      121 attggataaa gataactttt ttccaaagta atcttatttc cattaacctc aaatggaact
      181 tttttattgt agttacttaa tattttttct agcattttat cacttagttt aaattttcca
      241 attttcacat tctcaagact tacaactaag tttccatcct ttaaagtagg tctcaattca
      301 agttcatatt gactatttac caaaccaaat accttatatg gtcctgctac ctttatttta
      361 ccatttttca tttcaacaaa attattttca agttcattaa ttccatgctt ctgcatcaat
      421 gtatatatta aatttttaaa ctcttcctct gaaacagata tttttccaac cattctaagt
      481 ggatttttta ctacctccat agaatttata tatgagcctt ttgataaata ttcttcatct
      541 atgattttat tattttgtga actaacatca tatctttctt ttggtgtcaa gatatataca
      601 acaattccta caagtactaa aacaataaca attaaactta acaaaatctt tgcaaccttt
      661 cccataatcg tctcctttac aaatctgtca tcagatttaa aataaattta gataactaac
      721 ttcacataat attcttaaca atatacatac tataatacag tcttttaaaa attaattcaa
      781 ccaatctaat gttttgtaaa taaactgtaa aaacatgttt ttagtataaa agttaatatt
      841 ttgcataata ctcttaatta cacctttatt tttttctaat cttggattaa aattaatatt
      901 atgcaataca gtgtagtgtt ctgtattata atcaaattaa ataggaggta tagaatatgt
      961 caaatgaaac gaactcatct aactttataa agaatattat tataaacgac ttagaaacag
     1021 gaaaacatga cagtatcata actcgttttc cacctgagcc aaacggatat ttacatattg
     1081 gtcatgcaaa aagtatatgt cttaattttg gattagctaa ggagtttaat ggaaaagcta
     1141 atttaagatt tgatgataca aacccattaa aagaggatgt tgaatatgta gagtccataa
     1201 aagaagatgt aaagtggtta ggatttgatt ggaacgagtt aaattttgca tctaactact
     1261 ttgatgaaat gtataagaga gctttaatac taataaaaaa gggtaaagct tatgtatgtg
     1321 atttgacaca agaggaaatg agagaatatc gtggtactct aactgaacca ggaaaagaaa
     1381 gtcctcatag aaacagaact attgaagaaa accttgattt atttgaaaga atgaaaaatg
     1441 gtgaatttaa agatggtgaa aaaacattaa gagccaaaat agatatgtct tctccaaata
     1501 taaaccttag agacccaatt atatatagaa tatctcattc aacacaccat aatacaggag
     1561 ataaatggtg catatatcct atgtatgctt ttgctcatcc aattgaagat gcaattgaag
     1621 gaataactca ctctatatgt actttagagt ttgaagacca aagacctctt tatgattggt
     1681 ttgttaaaga atgtgagatg gaaaatatac ctagacaaat agaatttgct agacttaata
     1741 taaataatac tgtcatgagt aaaaggaaat taaaacaact tgtagatgag ggtattgttg
     1801 atggttggga tgaccctcgt gttcctacaa tatccggaat cagacgtaag ggatatactg
     1861 cagaagcatt gcgcaatttc tgtagtgaaa taggggtatc aaaagttaac tctacggtag
     1921 atagtcaaat gcttgattat ttcctaagag aaaacttaca accaaaggct cctcttacta
     1981 tgggagtttt aagaccttta aaacttatta taactaatta cccagaagat aagattgaga
     2041 tgcttgaaat agagaataat gctaaagatg aatctcaagg aaagagatta gttcctttct
     2101 ctagagaatt gtatatagaa caagatgatt ttatggaaga acctgttaag aaatatttta
     2161 gatttttccc aggaaatgaa gtccgtttaa aaggtgctta ttttgtaaaa tgtactgatg
     2221 taattaagga tgaaaatgga aatgtagttg aaatacatgg tacttatgac cctgaaacta
     2281 agagtggctc tggctttaca ggacgtaagg taaaatctac aatacattgg gttgatgcta
     2341 aatcagctat cccatgtgaa tttagattat ttgaaccatt aattcttgat gatatacctg
     2401 aaaatgaagg taaacatttc ttagaacaaa taaatcctaa ttcattggaa atattacaag
     2461 gttttgttga acctacacag ataaaagatg ctaaaccttt tgataaattc caatttgtta
     2521 gaaatggatt ctttagtata gataataaat atacaactga tgaaaaattt gtatttaata
     2581 gaatagttcc tctaaaaagt tcttttaaac caggtaaata atcttacaat caaaaaatga
     2641 agacttaaaa tataaattac ttctactata aataaactta gttagaagta attttttatt
     2701 tgagtcttca tcatttactt taaatgatta acttttttca ctacagattc ccatgacttc
     2761 tattccatct tttatgcatt ctatgttaag aggaaaatct ggtccttttt ctccgtcaat
     2821 atctgttata agtgaatctt cacattctat atgcaccttt ttagttttaa agtatagtat
     2881 ttcatttcca ttatattgat ctaaataatc acctctaagc acacttatca aaactggtat
     2941 tgattttggt attggcattc ctttaaaaat aattacatcc aaatatccat catctatttc
     3001 agccttatat gctagattta tatttcctgc tgttttgcca ttaaaaatca acataagata
     3061 catatctcca tcataataca tttcctcaga agatactttt atcttaaatt tcctcatata
     3121 caatgcttct tctattccct ttatataata agaaattctt ccaaatgaat tttttaagtc
     3181 tgtgtttatc ctttgtgata catccgtaaa cataccagca cttgcaacat ttataaagta
     3241 cttattattg actttcccta tgtctatttt ctttggtttt gaatttatga tttcatttat
     3301 agcttctttt atatcaaatg aaatttgaag agcatttgaa aaatcatttg cagttcctgt
     3361 aggcaaaatt ccaattggaa tttttatgtc taattctttc attgcattta ctactaaatc
     3421 tacagtacca tcaccaccag ctatcaatat gtgttcataa gaactatcaa cttccttaaa
     3481 tgcattcttt acaggttctt taatagttaa tctatagggt atcaataaat aatttttttc
     3541 ttgatataat tctatgatag tgtctaaatt tgataatatc tttttttcac ctgaattagg
     3601 attgtatata agttttactt ttttcattat ttctccttac tcattttatt cttttagtat
     3661 ccaaaatctt catttaaaaa aatcacatgt attctttctg gtgttaattg tctttttata
     3721 agtgtataac tacagcatat tcttcctgga cttgaacagt ctatacatat accagtagtc
     3781 ttacatccag tatttgtact taatctttta caattggctg gagcagctat tgtttttgtt
     3841 ctttttatag cttcatctac atctttaact attttattta ctcctgctat aactattact
     3901 ttatcaggtc catatatcat agcagcaact ctattcccat tcccatctaa attgtaaagt
     3961 tctccatcct ctgttatcgc attagtactt gtaaaataag catctgcaga aaatgacttt
     4021 ctatatattt ctttcatttc ttctggtttg atatcttttg catatctatc aagaaaatta
     4081 tatttttctc ttataaaatc aatcactcct gtttcaaata aggtcataga acctccacat
     4141 cctactagag aacctgcttt aactatttct tcaatttttt taagaagctc ttctttgtta
     4201 cttactaaat accctttcat attattgttt tccaaagatt ttattgtttt ttctattctt
     4261 ttttcattaa gccaatttaa attttgatcc attttaactt cccccttatt tttaatatgt
     4321 acttaattat ttataaactt tattttcttt ataagtttat tttagtacat ctaaaaaaaa
     4381 atacaatata aaacttaaat agttttacat aattttttcc aaaataactt actcaatcaa
     4441 aaaaggaaag tagatgtact attctacttt ccctatattg tatgtagtat ttattgccta
     4501 aaatctatat attaattata tgtttcatat tatataatat cacagttaat taaaatgtaa
     4561 tactatcttc tttttaaaca tataaattgt attattaaat ttaatatatc caacttcaat
     4621 aaatcttatt aatttgatga agaagatgct atagctgcac ttgttgctgc cattacacat
     4681 gcaatttgaa tagcaacaac aatatttaaa aatatattat tagttataga gcctatatta
     4741 ttctcatttc gtatcaaatc tgcataaatt gaagctacaa tagcagaact aatcattact
     4801 ctattacttt ttcctaaaga ccaatttcca aagccttttt cttctttaag tttatttgaa
     4861 acatttttta tctcttctat tatttcatct tctctataat ctaataatga aattgctcct
     4921 attattggat atccataata attcatttta caatcatttt ctttaaatgc ttttttaagt
     4981 tcaatagtct ttttacattt aagcatagtt ctatcatcac taaataaaag aatttgggat
     5041 aatgcttgta aatcatttcc tttataaaaa ccattttcat ttagaaattc ataacatttt
     5101 tctatgtatt ctaaatcttc atctaaattt tttgaatgaa ttgcaataag tgctatatta
     5161 caatagtcat cactagaagt tagaaatgga tgttttttcc tcatgtaatc atatattatt
     5221 ttcatttttg atatacatgt aagataatcc attttctctc tattttcata aactattgat
     5281 gctgtaagtg gtaaatatgt atcgccccaa aacttttcac ttttaagttc tttgtatata
     5341 tctaaaatca tcttaaaact actttctgga tttgattcaa acgaaagtag tgttgccata
     5401 taaaacatat tatatcctct aaaattagag aataatccag tatttttttt aataagctct
     5461 attcgttctt ccactaatct aggacttatt ttcttgttct taagggtaaa tgcaagtgca
     5521 caactatgtt gtatcaatcc catttgccaa gaacctttaa cttcatttaa tgctttgtaa
     5581 ttttctatta ataagtttac ttttccattt ataaacgatt gttccatatt gccctcctag
     5641 taatatatct cattgtacta atagttttat atttattata tatatattaa atactacaat
     5701 caatacatat tacttcaaag aacatcattc ttcaacaata atgtagcctt ttttgtaatg
     5761 tcttcccata ttattgactc tatcatagta ctctgattca ctttcattac tccatttggg
     5821 cacaaataca tctattgcat tagaattact aaaatactta ctagaccccc tatccataac
     5881 agtttttaat tttccatcta caataatctt agttccaaaa cttaagtgat tgtttgctac
     5941 aaaaccatct tctaaatcat ctcctgtagc agttatcgct ccatatccat tttcttgatt
     6001 aagacttgtg taataactaa cttcaaaagt tataggtact aacttcttta ttttaatacc
     6061 taactgattt tgtagtttct ccttttgtac tttcaactta tactcatcac tatttttata
     6121 atcttccata ccttgattat acttggaatt aaagctttta cctgacttta ctatagtatc
     6181 tatatttact atagattctt tacaatgatc tgcatctatt tttgtttctc catttaccaa
     6241 taatggagtt aaacatacac tacatacaac acatgtaatc aatgcttctt taagcttcat
     6301 aaattccccc cttattattt tgatatatta gcatatttaa tattatatgt ctacatttag
     6361 atttaaatac cacacccatt tgctttaaga agttgtctta tatttaattc accttaaaca
     6421 cttataaatc ctctatctta gaatcatata gaataaagct tttatcatta aatatttttt
     6481 attaatcttt taaaatctaa aactacttta tctaatt
//
LOCUS       DBGJFU010000170         6461 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.174, whole genome shotgun sequence.
ACCESSION   DBGJFU010000170 DBGJFU010000000
VERSION     DBGJFU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.174"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            352..1296
                     /locus_tag="ACOIAS_003440"
     CDS             352..1296
                     /locus_tag="ACOIAS_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HGZ2703108.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1318..1944
                     /gene="hisG"
                     /locus_tag="ACOIAS_003441"
     CDS             1318..1944
                     /gene="hisG"
                     /locus_tag="ACOIAS_003441"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGZ2703109.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1978..3027
                     /gene="hisC"
                     /locus_tag="ACOIAS_003442"
     CDS             1978..3027
                     /gene="hisC"
                     /locus_tag="ACOIAS_003442"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGZ2703110.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            3030..3608
                     /gene="hisB"
                     /locus_tag="ACOIAS_003443"
     CDS             3030..3608
                     /gene="hisB"
                     /locus_tag="ACOIAS_003443"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HGZ2703111.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3610..4215
                     /gene="hisH"
                     /locus_tag="ACOIAS_003444"
     CDS             3610..4215
                     /gene="hisH"
                     /locus_tag="ACOIAS_003444"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGZ2703112.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4212..4934
                     /gene="hisA"
                     /locus_tag="ACOIAS_003445"
     CDS             4212..4934
                     /gene="hisA"
                     /locus_tag="ACOIAS_003445"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGZ2703113.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4916..5674
                     /gene="hisF"
                     /locus_tag="ACOIAS_003446"
     CDS             4916..5674
                     /gene="hisF"
                     /locus_tag="ACOIAS_003446"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGZ2703114.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5702..6370
                     /gene="hisIE"
                     /locus_tag="ACOIAS_003447"
     CDS             5702..6370
                     /gene="hisIE"
                     /locus_tag="ACOIAS_003447"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGZ2703115.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2643 a          545 c         1263 g         2010 t
ORIGIN      
        1 tttaaataac tattgccaat ttttgaaaaa tatattatta ttaatagtaa caaaaaactg
       61 taaatttaga atattaataa aaattatttg ggaggaaatt atcatggtga gattaaatat
      121 agtaaaatta aatacaaaat taaatcatca ccatcatcat agattaaagg gattgtaata
      181 tgaaattaat tcatagacac aagccctgtt tttagtgtaa ttggtcttgt atctatgatg
      241 ggaagtaatt ttaagagaaa attatagcca tataggtata aagcctatgt ggctttttta
      301 ttgttttaaa tttgcaaata taagagtaaa tttaatgaag agggtgagaa tgtgaaattt
      361 ttgaggattg aagatgaggt tatttattct aaaaaaagat ataaattaaa aagggaaata
      421 gataaacttt ttttggatga agaatatttt caaatagaac cacaattatt tgaagaatat
      481 gatgaattta caactataaa taataaaatt ccagaagaat ctatggtaaa ggttgtaaat
      541 ggaaaagtaa tggtacttag agctgatata actacaagta taataaaaag ccttgtacca
      601 agatgggaag atggtttaaa gcttaagctt ttttataatt catcaatata taaaaataaa
      661 aatacagttg gaataaaaga gataagacaa ataggttgtg agtatctagg agaagcctct
      721 gtagaagcag atagagaagt agtgaaatta gctcttaaga tacttgaaaa atacaataat
      781 aattttattc tagaggttgg gagtagcaag tatattcatg gtttgttaga agaattaaat
      841 ttaaataaga attgtgaaaa tcaaattaaa aatttactat atacgaagaa cactcatgaa
      901 ctaaaagtat atattgaaga attaaaaatt aagcgtgaag taaaagagtt attatcaaat
      961 attttaagtt tacaagggaa tttatataat gttattgaaa aatcaaacaa gttctattgt
     1021 aataataaga tgaagcaagc tcttgaagaa ttaaagcaag taaataactt gattgaggaa
     1081 tgtaattttt tagataaggc tagatttgat ttatcaatga ttactatgct tgactattat
     1141 gaaggaatta tgtttagagg atactaccca aactcatata aagaaattct tagtggtgga
     1201 agatatgatt ctttaactaa agagtatgga aaagaaattc cagccattgg atttacacta
     1261 agtgttgatg agttaatgaa gtatgtatat aagtaaagtt tagaaggagg gattgctttg
     1321 gattatttga ctatagcact agcaaaagga cgaattgaag gggaatcatt taaaaaattt
     1381 aaaaaaatgg gtctaggaga cagtatagac acagatacga gaaaactaat ttttaaagat
     1441 gaagaaaata aaattatata tatacatgta aagccatcag atgtagttac atatgttgaa
     1501 aaaggagtcg ctgacctcgg tatagcaggt aaagatacta ttttagagaa tgagacagat
     1561 gtatatgaaa tatatgattt aggttttgga aaatgtaaat ttgctgtagc aggattaaag
     1621 ggagatagca tatatagaga agatgaatat ttaaaagtgg caacaaagta tcccaatata
     1681 gcaaaaaagt attttaaaga gaaaggtcaa aaaattgaaa taataaaatt aaatggttct
     1741 gtagaactag ctccaatagt agggttgtca gatgtaattg tagatatagt tgagactggc
     1801 aatacactta aagcaaatgg tcttgaaata ttagaagaca tatgtaatat aagtgctaga
     1861 attatatcaa atagagctag ctatagattt aaatatgaac aaattcagaa tataattcgt
     1921 ttatttgaag aacttgataa ttaaaatata attgagcttt ataaggaggg atttaagttg
     1981 agagaaaaag aaagcataag agagttaaag ggatatgaac caaatcacgt aaactgtaaa
     2041 gtaaaacttg atgcaaatga aggaagtaag aggttgttta aataccttat taaagaaatt
     2101 tcagatagtg atatagattt aaatttatat cctgaagatt cttatagtga tttgaaagaa
     2161 tctataatag attacataaa tatttctgga gtaaataaga aaaatcttct agtaggaaac
     2221 ggttcaagtg aaataattga tttaataata catacttttg tagataaaga tgaagtaata
     2281 ttgagtttct ctccaagttt tagtatgtat tctatatata gtcagataaa tgggtctaaa
     2341 tttataggtg ttgaaagtga tgaaaattta gttataaata tagataatgt gattgaaaaa
     2401 gtaaaggaga ataatcctaa aatagtaatt gtatgtaatc ctaataatcc aactggaaca
     2461 atattaaaga gggaagagat aataaagctt ttagattcaa ctaatagtct agtggtttta
     2521 gatgaagcat atatggattt tggagaagaa agcatgttaa gtgatgtttt taagtatgat
     2581 aatctaatag ttcttagaac tttatcaaag gcatttggat tggctggaat aagaacagga
     2641 tatatgttat ctaatagtag tttaataaat tctgttgaga aagtaagacc tccatataat
     2701 ttaaattcat tatcagactt tattgctaca agagcactta gaaataaaga tgtggtaaag
     2761 gcttatataa aggaagtaaa agaggaaaga gaagttttat ataaagaaat gattggtatg
     2821 ggaattaaag catataaatc acaagcaaat tttatattat tttattctga aatcgaaaat
     2881 ttatcacaaa aacttattga tagaggtgtt ttaataagaa aatttggtgg aaagttagaa
     2941 aattattaca gagttacaat tggtgataaa gaagaaaatt ctatgtttgt tggagctata
     3001 agagatattt tgaaaaagga gaaataggta tgagaatttg gaaggtagaa agaaatactc
     3061 ttgaaacaca gattttagta gaattaaata ttgatggttc tggtaaagct gaaatagata
     3121 ctggaatagg atttttagac catatgctta ccttaatgtc atttcatggc aagttcgatt
     3181 taaaagtgat ttgtaagggt gatacatatg tagatgacca ccatagtgta gaagatatag
     3241 gtatagctat aggtgaagca tttaaaaatg ctctaggtga caaaaaagga atacgaagat
     3301 attctaatat ctatatacct atggatgaat ctctatcaat ggtagcaata gatataagca
     3361 atagacctta tcttgtattt aatgctaaat ttgataccca aatgattggt agtatgagta
     3421 ctcaatgctt taaagaattt tttagagctt ttgtaaatga atcgagagta acattacata
     3481 tcaatctctt atatggggaa aatgaccatc ataagataga aagtattttt aaagcatttg
     3541 caagagcttt aaaagagggt agtgaaatag tttctaatga gatagcatct tcaaaggggg
     3601 ttttgtagaa tgaatataat agtagactat ggtcttggaa atatagattc tgtgtctagg
     3661 ggttttagaa aagctggaat agaaactaaa atttctagtg atatagatga aataaaacag
     3721 gcagattcac ttatacttcc aggtgttgga gcttttagag attctataag tgccttagat
     3781 aaactaggat tgataccaat aataaaggag catgtttcta agggtaaatt tatgataggt
     3841 atatgtctag gaatgcaatt actttatgaa aaaagttatg agtatggaga atatgaagga
     3901 ttaggactta taaaaggaag tattgataaa ctggatataa gtttaaaagt gccacatatg
     3961 ggatggaata atctaaaatt taataaggca aatgatgata tacttaaata tataaatgaa
     4021 gatgattatg tatacttcgt tcactcatat tatgcaaatt catcaaatga agaactaatt
     4081 gcattttcag aatatgaaaa gaaaatccct gctattgtta gaaaaggcaa tgtatatggt
     4141 atacaatttc atcctgaaaa aagtggagaa gtagggttaa atatattaag agcatatggg
     4201 gagatgataa aatgataata tttccagcaa tagatataaa agataataag tgtgtaagac
     4261 ttacacaagg agaatttgac aaagtaaatg tatattatga taatccatta gaagtagcat
     4321 ataaatggaa aaatgaagga gcagaatata tacatatagt agatttaaat ggtgctagaa
     4381 gtgaatttgg tgtaaataca aaaataattg aggacatagc aaacaatata gatataccta
     4441 ttcaagttgg cggtggagtt agggataagg aaaaagtcaa aagtttaata aatgctggtg
     4501 ttactagagt aatattaggt agtatagcga ttgaaaattt gaatttagtt gaagaattgg
     4561 tcaatgaata taaagagaaa attgtagttt ctatagatgc taaagatgga aaagtagcag
     4621 ttaggggatg ggaagttgta agcaatgtag actcattaac attatgcaaa caacttgaaa
     4681 agataggtgt acaaactatt gtttatacag atatatcaaa agatgggatg ttgcaaggtc
     4741 caaattttga catatatgaa agaatagcaa aggaaacatc actaaatgtt attgcttctg
     4801 gaggcgtaac ttctatagaa gacgtaaaaa gactaaaagc tatgaactta tatggtgcaa
     4861 taataggtaa agcgctttat gataaaaaga tagactttaa ggaggcacaa caattatgct
     4921 tactaggaga ataatacctt gtctagatgt gagaaatgga agagtcgtaa agggaaaaaa
     4981 atttaaagat atagtagatg ttgatagtcc agaagtactt ggaaaatttt atagtgattg
     5041 tggtgcagat gaacttgtat tctatgatat aactgcatca aatgaagaga gaaagacatc
     5101 tttagaattt gtgacaaaag ttgcagaaaa tataaatata ccattttgtg ttggtggagg
     5161 agtaaataaa ttagaagatt tcactgatat tttaagaaaa ggtgctgata aagtttctat
     5221 aaattcatca gcagtcaaaa atcctgaatt aataagggaa gcttcattaa aatttggagc
     5281 acaatgtgtt gttttatcga tagatgcaaa gaaaaatgaa gagggttcat ggagcgtata
     5341 cgtaaaaggt ggaagagaaa aaactaatct ggatgcaatt gaatgggctg ttaaaggtgt
     5401 agaacttgga gcaggagaaa tcgttgtaaa tagtatggat gaagatggaa tgaaaaatgg
     5461 atatgatata gaattattat caaagataac ttctttagta aatgtacctg tcatagcatc
     5521 tggaggcgct ggaaaaaagg aagattttta tgaggcggtt aataaatcca atgtagatgg
     5581 aattttagca gcatctgttt ttcattttgg cgagataaaa ataaacgatt taaagaaata
     5641 tttaaaagac atgggtgtag aagtaagatt ataagttaaa tactaaaagg agattagaaa
     5701 tatggataat aagtgtaaca atgtatacag tgatgaagta gagaaattta taagaagtat
     5761 aaagtttgat gataaagggt tagtccctgt tgttgttcaa gaggtagtta gcaaagatgt
     5821 actgatgtta gcatatatga ataaagatgc tataaagaaa accttaaaag ataaagtagc
     5881 ttgctatttt agtagaagta gacaagaact ttgggttaaa ggtgagacat ctggtaatac
     5941 tcaaaaagta gttaagatgt cttatgattg tgatgtagat actatactac tttttgttga
     6001 acaaactggt gtagcttgtc atacaggcaa ttattcttgt ttttacagag acttgtttga
     6061 tgatagttca aaaatggagc ttgaagtaca aacaaatata ttgaaagaat tatatgattt
     6121 aatcaatgaa agaaaaaata atccagttga aggttcttat actaattatc tttttgaaaa
     6181 aggaatagat aagattctta aaaaagtagg tgaagagagt agtgaggtaa ttatagcatc
     6241 aaaaaatacc gataaaagtg aacttattta cgaaattagc gatttagtat atcatacatt
     6301 ggttttaatg atagaaaaag gtgttgaaat tgatgaaata aaaaaagaat tacttaaaag
     6361 aagaaaatag aataagatag attaatgggt aataagaaaa tataccactt ttaataaaaa
     6421 gtggtatatt ttttatttta tattttgatt tatagaataa t
//
LOCUS       DBGJFU010000171         6448 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.175, whole genome shotgun sequence.
ACCESSION   DBGJFU010000171 DBGJFU010000000
VERSION     DBGJFU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.175"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..1752
                     /locus_tag="ACOIAS_003448"
     CDS             <1..1752
                     /locus_tag="ACOIAS_003448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703116.1"
                     /translation="RIFMVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTI
                     PFEKMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKI
                     RNNIDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLK
                     NIGKLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVK
                     IYSDYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQ
                     DVKDYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDL
                     YDYSSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNN
                     KMQPSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQE
                     IIYKNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLA
                     SDVSLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISST
                     LYDYFGIDNPLELKNAS"
     gene            complement(1916..2110)
                     /locus_tag="ACOIAS_003449"
     CDS             complement(1916..2110)
                     /locus_tag="ACOIAS_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703117.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2107..2688)
                     /locus_tag="ACOIAS_003450"
     CDS             complement(2107..2688)
                     /locus_tag="ACOIAS_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2703118.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2856..3674
                     /locus_tag="ACOIAS_003451"
     CDS             2856..3674
                     /locus_tag="ACOIAS_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2703119.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4349..5170
                     /locus_tag="ACOIAS_003452"
     CDS             4349..5170
                     /locus_tag="ACOIAS_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2703120.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGNVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEIWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5480..6259)
                     /locus_tag="ACOIAS_003453"
     CDS             complement(5480..6259)
                     /locus_tag="ACOIAS_003453"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGZ2703121.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2428 a          876 c          825 g         2319 t
ORIGIN      
        1 aggattttta tggttaaatc caagaagaaa atatttgttt ttctaatttt aattgcagtt
       61 tttattatat ttgcatcata cataagaaat tatactaatg atacaaaaaa agattctgtt
      121 agaggaacta ttccttttga aaaaatggaa tataagcgtc ctaatataca gtcaatttgc
      181 gaaaacatta acaagtgtaa cgacaaactt ttaacagcta aaacagctaa ggaacaatta
      241 aatttattta atgaagttga taaaatttat caggattttt acagtacact tactatagca
      301 aaaattagaa ataacataga ttcatcagac gaattttact ctaaggaata taagtatctt
      361 atgagtaaat cagtagatgt tgacatgtca tacaaagact ttcaagatag atttgtaact
      421 tctaaattct ctaaagaatt agaaaaagaa cttggaaagg atacatttaa ttatctaaag
      481 aacattggaa aacttaattc taaagaagtt gaaggtttgc ttaaaaaaga gcaagaccta
      541 gttgtaaaat atgaagattt attagctaaa tctacagtat caatagatgg tatagaggta
      601 gactttgaag aagccttgtc aagaccaaat cttagccctg aagaatatgt gaaaatttat
      661 tcagattact taaaaaaata caatcctata tttggtaaca tattcttaga acttatacag
      721 acaagaactg aaatagcaag taagcaagga tttaagaatt atatagatta tgcctatatg
      781 aacctaaaca aagactattc tcaagaagaa gctaaaaaat ttagacaaga tgtaaaagat
      841 tacatagttc cattatatag agagatttca tctaaaccat ctgattcttc tatttatatt
      901 aaagtatata aaaatcgctc atttaggaag tttgataaag tacttgaaga tatatctcca
      961 aaactaaaag agagcttcga ctatatgaaa aaatatgact tgtacgatta ttcttctgga
     1021 aaaaacaaat cacctggagg atatactact tatataaata aatataaagc acctttcttg
     1081 tttaatacat gggacaattc ttttttaggt gttactagtt ttgctcatga gtttggtcat
     1141 ttttataatt actacaacag tattaactta aataataaga tgcaaccaag tatagatgta
     1201 tgtgaagttc attcaacatc tttagaaatt ctattttata aatattttga tgattttttt
     1261 ggtaaacaat ctgaagctat aaaaaaagaa cacttatcga ttgttttaaa tactattatt
     1321 gatgcttgtc tttatgatga atttcaagaa attatataca agaatccata tatgagtcta
     1381 aatgatatca ataaattgtt ttttgattta gaggaagaat atggagtttc caataatata
     1441 cttagagaaa aaaatgctcc tttttggata cttgtatctc ataacttcca agttcctttt
     1501 tattacttga gttatggtct tgcatctgat gtatcacttc agatatggca attatcacaa
     1561 gaagattaca gaaaagctgt tgatgtatat atggattttt taaatcaaaa tactgatgct
     1621 ggatttaaag atgtggtaga aaaggtaaat ttacaattac ctttccaaaa tggaaattta
     1681 gaggaaataa gctctacttt atatgattat tttggtatag ataatccact tgaactaaaa
     1741 aatgcttctt aatttctaat actttatact cactcttaag atttagtatt agattgaaat
     1801 aagtgatatt ttttcacaca caattaatgt ttaataaaat tatatggtaa agtatcagaa
     1861 atttttaaga ctcttgtctt tataatttct gatactttat ttattaataa tattattatt
     1921 tttttatata tataattaaa aggattgtgg aaatcaataa aaatatccct gtaaaaatag
     1981 cactattcat atggaactta atagaacttg catctgttac cttattattc ttaatccaga
     2041 acccacaaac catagttgaa agaaaaagta aaacagtgat aagacttgaa ccaatagtta
     2101 taattttcat cttacatcaa catcctctcc aataatattt tctattaaaa tatcaatgaa
     2161 tttatctcgc tcatatttat tctttatatt tataccactg tataactgaa atgattctgt
     2221 agataaagca gaaatctgta ttggtaaaat aatttgcagg gcaatgattc ttagactcat
     2281 ttcatctttt ttatttccaa aaattttacg aagcataggg acaatatcaa gttctgcttg
     2341 catatctcca ttggttatac attgattcaa tacaaaagga agcacttttt cataatgtaa
     2401 tccagtatca cacaattttt ttaataaatt ttttaaatct tcaataggtc ttagagtata
     2461 tacactatcg tcatatagct ctgctattat atttgacatc acatctccta tggcagtact
     2521 caacaaatta tcctttgttt taaaatgata atttattaaa ccaactccaa catttgcacg
     2581 ctcagcaatt tttctaactg taatctcctc aatatcatct acttcattta aaagattcaa
     2641 tgtaacttca attatacgtt ctctagcatc tttatcatta cttttcataa tagacctcca
     2701 aaacaattaa cattttcggt tctgaacatg tttagtataa tatttttttc atcatatgtc
     2761 aatataattt aattataatt ttagttgact ctaacattat gatatggttt atattaatat
     2821 taagctaaaa gtacctttat aaaggagaca aaaacatgtt taagataggc gatttttcaa
     2881 agctaagtaa aattagtatt cgtatgttac gtcattatga tgaaatagga cttttaactc
     2941 cttctcatac gaataaaact aatggatata gatactatag tgcagaccaa ctttctacta
     3001 caaacagaat ccatgcacta aaagatatgg gctttggctt gtattctata aaagaaattt
     3061 tgacagaata taacgataaa gaaagtctca taaaatatct aaatatccac cattctcaag
     3121 taaaagaaca attagaagat acacagaaaa aactattaaa aatagaaacc actattaaaa
     3181 gaattggagg aaatgatatt atgaaaaatt atgatgtaac tataaagaat tttgcaccaa
     3241 aatatatgat gacacttaga agggttatac caacttacca agacgaagga atgttatggc
     3301 atcaagtatt tttagagaca aaagaccaga atgttcaaat tgaaccccca aagtattcta
     3361 aagcagtttt ttatgatact ggatataaag aagattatgt agatgttgaa gtacaagttg
     3421 ctgtatctgg aaagtataaa gatactgaac atgttaaatt taagactgtt ccaagtgtga
     3481 ctgctgcaac tgcaattgtt aatggaaatt tcaatcaagt tgcagacgct tgtgaggcta
     3541 ttggtaattg gatatcagat aataactatg atgtggatgg tcctatgttt aatatttatc
     3601 atgtaagccc tggcaacgat agtaatcctg ataattgggt aacagaggta tgcttcccag
     3661 ttaaaaagaa gtaattttta tattaattgg ataatatttt aatttgttag acaaattact
     3721 tttagtgtta tgtataaata cctacaattt gattaggata ttatcttaat ttgttaaaca
     3781 aattactttt agtgttatgt ataaatatct acaatttaat taggatatta tcttaatttg
     3841 tcagataaat caattataag attatatact atccttatac tctagttaaa tatattttaa
     3901 tcactaatat aaattagttt ttttaacaaa acaataaaag agtaaagtac aaataattcc
     3961 tttgtgctct actcttttta actttcttta gacaataata caatgataaa tataaaaaat
     4021 tatatattcc attctataga aatatgctca ccaataacat gtatgttgtt aatcaatata
     4081 ttatatcaaa ttatatcaaa attacataaa ccttatagga caatcatatc ttattacaag
     4141 aaaaatgtct tatccatatt gaataagtca ttcagttcat atacaaagtc aatttcattg
     4201 agaaacattt aatatatgtt tataaaaatt tcatactatt ctttcatatt tacatgtgat
     4261 ataatttatt aactcataat tttgttaatc agttgtgatt gtagattatt tatataatct
     4321 aatattttaa gaaggtggtg taataacttt gaaattaaaa attgttggta gtggcggtat
     4381 gtcaattatt ccaagttcat tttgcaaatg caaaatatgt gaagaagcca gacaaaaagg
     4441 cggaagatat gaacgattag ggccaagttt atatattgat gatataaaaa tgctgataga
     4501 cacacctgaa gatattgcag tagcttgtaa cagacaaaaa atatcggaag taaaacatat
     4561 ttcaatttct caccatgacc cagaccatgt taagggtatt agaattgtcg aaaaaatagg
     4621 atgtgacttt atcacaggag aaaataatcc aattggcttt tatgcgcttc ctgaagtaat
     4681 tgaagatata aaccgaacga atctcgattg tcttaaatat tatggtaatg ttataaattg
     4741 catttctatt aatgaaactt cgcatgtaaa gataaataat tttgatattg atttgattaa
     4801 caataaccca gacaggaata taaccttcta tgttatcaaa gagaatgaaa aaaaagtcat
     4861 atatgcctgc tgtaatccca agccatttac tcatagtgat atgtactttg atgctgatgt
     4921 tttaataata agtctagttt ctgatgatgg tatattaaat gatggtacaa aacttaaaga
     4981 cacaccattt aaagatgaaa tatttacttt agatgagatt gttgaaatta aaaactatta
     5041 tagaattaaa aagattatca ttacacatat tgacgaaata tggggaaaat catatggtta
     5101 ttacagtgaa ttagaaaaga aacttgataa catttatttt gcatatgatg gtatggaaat
     5161 tatagtctaa atcattaagt aataagcata atgcaaaata agaaaacttc aatttaacag
     5221 tatagttaaa ctatgtaata tcaacatatc tcagttttta caaaatatca tagtatttat
     5281 ctttataaaa tttcaagtcc tacatatttt aatacttcaa tatctaaata aagtaaaatc
     5341 aaaaatatat tttatataca ttatagacgg cttatcggca ctagataagc cgttttgtca
     5401 caattattat ttgctcaaca aaacttctca taatatataa cagattgaat ctccaactga
     5461 tacttaatta ccgaaactct tattccttaa tatgttgttg cattaaccaa accttcatca
     5521 tcattttttg tggcaatagt tttgctaata aatgactgga ttttgtattt ataccataca
     5581 cagaaatatc cttatttttc tttgcatcct tcaatgcttt tttggcaaca atgtctggag
     5641 acactatgcc tgggaaattt tttgtccctt tttcagctac aactatacct ctcttaaata
     5701 gatttgtact catccaacct ggacatacag caattgcatt tatcccttta ctcttaagtt
     5761 caacatttaa tgctcttgta taatttctta taaacgcttt agttgaacta taaatatttt
     5821 gatatggcaa tggctggaat gatgcttgtg aagaaatatt catgatatgg cttccctttt
     5881 gcatatatgg aatacatata agtcccaatg ctacaacagc actaatattt aaatcaatca
     5941 tattaataga ttcatctata cttaaatcat tgtatgaaca gaattttgca aaaccagcat
     6001 tgtttataag gtattttata catacatttt ctttacttaa aaatgtacca atttctttta
     6061 cttcttctat ttttgataaa tcttttgaaa aaatttttac cttacttcca aattcatcta
     6121 ctaattgatt taatctcgcc ttgtttcttg caactgccca tatttctgtt atttcttctt
     6181 catttattaa taactttaga aactctttac caaaaccact attagcaccc gttataattg
     6241 caatcttctt ataactcata attattctcc tttaccataa gtttatacgc tcaaactgta
     6301 aacttatttt ttacaaataa aacaccctct attcagatgt tatattcata ttatttgaga
     6361 tattatattt atatcatttt caataattgt tatatagcat aaaaaatagt gttaatatta
     6421 tagaatcggt attgattata tttaaaaa
//
LOCUS       DBGJFU010000172         6366 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.176, whole genome shotgun sequence.
ACCESSION   DBGJFU010000172 DBGJFU010000000
VERSION     DBGJFU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.176"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..833)
                     /locus_tag="ACOIAS_003454"
     CDS             complement(<1..833)
                     /locus_tag="ACOIAS_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGZ2703122.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SP"
     gene            974..2401
                     /locus_tag="ACOIAS_003455"
     CDS             974..2401
                     /locus_tag="ACOIAS_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGZ2703123.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIAKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFNWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2597..5191)
                     /gene="clpB"
                     /locus_tag="ACOIAS_003456"
     CDS             complement(2597..5191)
                     /gene="clpB"
                     /locus_tag="ACOIAS_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGZ2703124.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5228..5824)
                     /locus_tag="ACOIAS_003457"
     CDS             complement(5228..5824)
                     /locus_tag="ACOIAS_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703125.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(5841..6245)
                     /locus_tag="ACOIAS_003458"
     CDS             complement(5841..6245)
                     /locus_tag="ACOIAS_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HGZ2703126.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2033 a         1039 c          683 g         2611 t
ORIGIN      
        1 ggagatatat tataattctt tttaaataag cgactaaaat aatttatgct atcaaagcca
       61 catctcatag caatttctgt catatttaaa tctgtttcct ttaaataata tatagcttta
      121 tcaagcctga ttccatttat aaaatcgatt gtagtcttac ctgttatttg cttaaatgtt
      181 ctacaaaagt gatgtgcact caagttcaca cgactagcaa gttcttttaa tgtaatttta
      241 tccatatatt tttcttctat caaatcaaag atacattcaa atttttctaa agaatacttt
      301 ctttttataa attcatcttc agaatatatt ttatcaatat gacttcttaa taagagtact
      361 attaattgat agatattagc ttttatagat aactcatatc caacttcttt attgtgatat
      421 tcatcaagta tagttgtaat acattttaaa atcagagaat cattttgaat taaattatta
      481 aatacaatat gatttaaagc caatggtgct aagtacttgg tttgtaacaa atcaacttga
      541 ttactaaaaa ggaatgtggg ttcaatacga atactataga attttaaatt atctgataag
      601 ctttctaaat aatgtaattc attactgttt gctattacaa tatttccagg cactacctta
      661 aaattatcca atccacattc aagatatgct ataccatcaa taaaataata tatttgtaga
      721 tgctcatgcc aatgtctttt aaagtttgct ccttttttaa tgtcatcctt taaaaaaata
      781 tctataaagt tttcttgacc tttcctgtta aaatatgtat ttttatcctt cattattccc
      841 ccctcatcaa aatagtgcta attataagca atatagttat tgtattcatt atttcatatc
      901 gctattatat aaaatgtaac tatcattgta aacattaaca taatgcagtc attataataa
      961 ggaggtaata aaaatgacaa actcttttaa aaaagatgat aatagaaaaa aaatcacagt
     1021 tttatttgta gtcattgcca tgacttttat ggcaacatta gattctagta ttataaatgt
     1081 tgctctccca gttcttgcaa gcaaactaaa tgtatctcta gcatctattg aatgggtaat
     1141 agcaagttac tcaattatta tttgttcaac actattattt tttggaagac ttggagatat
     1201 tattgctaaa tcaagagtat ttcaagtagg tactattctc tttacatcag cttcattact
     1261 atgtggatta agtaattctt taactttact tatagtttgc cgctttattc aaggaatagg
     1321 cgcttctgcc tacatggcaa acaatcatgg aattatcaca gaactatttc caaaagaaag
     1381 cagaggaaaa gccctaggga ttttagttac tgcagttgct attggaaata tggttggacc
     1441 atctgtggga ggatttattt tgtcaatatt taattggaat gttatatttt ttataaatat
     1501 tccaatcggt cttattgtaa tctttttaaa tacaaaattt cttcctaata gtaaaaaatc
     1561 ttcagaaaat atggataaaa ctggtgctat acttcaattt ttaggaacaa cattattttt
     1621 tagtgctcta attagtgctc aacaaacagg attactaaat ccatatatat taattgcact
     1681 attattatca attattttta ttatattatt tttgatatta gaaaaaaaac atcctcaacc
     1741 gcttcttgac cttgaaattt ttagaaattt taaattttca ttgaatttaa tttgtgctct
     1801 tacatcattc atttgtattg catcatcaag tattttaatt ccattttatt tacaaagtac
     1861 tatgaaactt ccccctattc aagcaggttt atttatgata ctatcacctt tgattttagc
     1921 cattttttca cctatatttg ggaatatatc agataaaatt aagtcagaaa aaattatttt
     1981 aattggtctt ctcgttatga gttttggatt ctttctaatg tcaagattga aagaaagttc
     2041 tgcccttatt ttatttgtaa tttatatatc gataatatca ataggacaag ctatatttca
     2101 acctgctaac aatgctttga taatgtcaag ctgttctaga agtaagctag gagttgtagg
     2161 aagcataaat tcccttgtaa gaaatttagg acaagttata ggtattacta tttctacaac
     2221 acttttatat aattttatga gtattaaagc tggatatagg gtcaatgatt atgtgattaa
     2281 taatgataaa atatttgtat ttggaatgag aaatgtttat attattgtta cacttgtttg
     2341 cttaataggt gcaatattaa ttggctttta tttatttaaa tataataaaa atgaacaata
     2401 gtttttataa ttatattcaa attatgatta tattataaga aatttactaa aatacttcct
     2461 ttgagttaaa tttaatttgg acatattgac ttaaaaatgt tttattaatt tgataaaata
     2521 tttcttttcc tatagtttat aaataaaaat cagctgagca aaatatttag tatattatgc
     2581 tcaactgatt cttttattat ttaactaaaa cttcaatatt ttcatctttt ccatctataa
     2641 ctatagtaca tccattatac acatctccag caataagttt ctttgcaatt atagtttcta
     2701 atgtatttcc tatatatctc ttaagaggtc ttgctccata tacagggtca tatccttctc
     2761 tcaccataac atcttttgct gaatttgtaa cttcaatagt aatatctttt tcttttaatc
     2821 tattttttaa atctttcata aatatatcta taattttctt gatattttct tggtctaaag
     2881 gcttaaacat tataatatca tctactctgt ttaaaaactc aggtttaaat ctatgcttca
     2941 tttcattcat tactaaattg ttagtagtct ctgttatatt tcctcctgct tctaatagat
     3001 aattacttcc tatattagaa gtcattatta taagagtatt tttaaagtct actgtcttac
     3061 ctttattatc agtaagtcta ccatcatcta atatctgtaa gaacatgtta aatacatcct
     3121 catgtgcttt ttctatctca tcaaatagta ttacagaata aggagcacgt ctaactgctt
     3181 ctgtaagttg accaccctct tcatatccca cgtaccctgg aggaggtcca actagtcttg
     3241 atacagcatg tttttccatg tattcagaca tatctattct tattatatta tcttcactat
     3301 cgaataaatt tctagcaaga gtttttgcaa gttcagtttt accaacacct gtagggccta
     3361 agaaaataaa tgaaccaatt ggttttcttt catcttttaa tcctgctctt gcacgtatta
     3421 ctgcatttga aactgctgta actgcttcat cttgtccaat aactctttta tgaagttcat
     3481 cttctagttt aagtaatttt tctctttctc cttcaactag tttagttact ggtatacctg
     3541 tccactttga tacaatgcta gatatttctt cttcagtaac ttcttctttt aaaagagaat
     3601 tttcagaacc atcattcata ttttcttcgt actgctttat ttgttcttct aacttaggta
     3661 tttcaccata tttaacctct gctgctttgt tgaaatcata ttctctttca tacttttcag
     3721 ctttaccttt agcttcgtct aattgagcct ttaagttttt aatatctaat atttgactct
     3781 tttctttttc atattttgct gtcatttcat catttttaga ttttaactct gctatttctt
     3841 tttgaatatc atccaatctt gatttacttt tatcatcatt ttctttaagt aatgcttctc
     3901 tttcagtttc taatgtaaat aactttcttc taacaacatc taattctgtt ggcaatgaat
     3961 ctatttcact acgaatcata gctccagctt catcaattaa atcaattgct ttatctggta
     4021 aaaatctatc ttgtatatat ctatgagaaa gttttgctgc tgctacaata gcattatcat
     4081 gtattcttac tccatgatgt atttcaaatc tttcttttaa acctctaagt atagatattg
     4141 tatcttcaac agtaggttct tctgcaatta caggctgaaa acgtctttca agagccttat
     4201 ctttttctat atactttcta tattcatcaa aagtagttgc accaatacag ttcaattcac
     4261 ctcttgcaag cataggttta ataagattac ctgcatccat tgaaccttct gttttaccag
     4321 ctccaacaat agtatgaatc tcatctataa acagtataat ttttccatca gaactttgaa
     4381 cttcttttaa aactgccttt aatctttctt caaattcacc tctatatttt gcaccagcaa
     4441 ttaaagctcc catatcaagt gagaatacaa tcttatcttt taaaccttct gggacatctc
     4501 ctcttactat tctctctgct aacccttcta caattgcagt tttacctaca cctggctcac
     4561 caatcaatac aggattattt tttgttcttc ttgataaaat tcttataatc cttcttattt
     4621 cttcatctct cccaattaca gggtctaatt tatttttctt ggctaaatct actaagtttg
     4681 ttccatatct agcaagtgca tcatatgtgc cttctgggtc ttgtgtttct actctttgac
     4741 tacctctcac ttgtgataaa atatttaaaa agtcattctt atttatatta tattgtttta
     4801 atattttacc aacagctgat tttgattctg tttccatcat tgcaagcata acatgttcta
     4861 cacttatata tgaatcttta aattctttag aaatactctc tgcctttata agcacctcat
     4921 ttatttttct agtagctgat actccttgtg agtcagcacc ttctccatat acttttggaa
     4981 gcttatcaat ttcaaagttt acagtatttc tcaaagaatc aattgatata ttcatttttt
     5041 cgattatatt tggtatcaat ccatcctctt gattaataag tgctgagaaa agatgaatta
     5101 catctacttg ttggttatga ttttttactg cttcgttata agcctcattt aaactttttt
     5161 gaactcttaa agtcattttt tctacgtcca tataaatccc tccattttaa tattatatcc
     5221 aaataaatta aattctctca tttataatta ttgattttct aagctctaaa agactatcaa
     5281 gtaaattctc gtttttttgt atatcgaaac tcaatgttaa attaaatgtt ccatctgaat
     5341 taaaaaaatc gaatacttct tttgattttg ctattaaata caagtcttca tctataagtc
     5401 ttttttcacc tattaataaa tactgacatt tatctataaa actcttttta aaatcaatag
     5461 tgtatagatt ttctaaaata ttatattgat aaatagcaat attattaaac acttttaata
     5521 tgtttgaatt tacaaaattg taaatttgat tattgttata cagtgatatt tgttcgtata
     5581 tatattccct aaataaagga tgaatgtttt gcttaagttt taaagtaact tcagctggta
     5641 tttcaaaata aagcttccag tacttagagt tatttttttc taaaataaaa gctactttgt
     5701 tcaaactggt agaactaatc tcctttagtg tacaattaaa attatagtgt ctcaaactag
     5761 ttttttcatt ctcaaataca tagtcaaacc actgtagaac atttctatca agttttaaac
     5821 tcatataaaa accttcttcc ttatttacta accttattag gtctttttat ctgattatat
     5881 tctgctaata aactttcttt atcagctcta ctacctttat atgctatatg tggatttatc
     5941 atatactcac catttttcac tctttgtaaa aaatccattt ttttcagctc cgtaaacaat
     6001 gaagataaag aagtttttgg atattcaaat tcacaagata attcatataa tgtttttcta
     6061 atcttattat ttttatccat agagtttatt aattttaatg gaattaaatc cctcttagta
     6121 tacattcctt ctactacttt aatataattc ttaaaacaaa cattcatata cttactttta
     6181 ttcataacta aacttttgct ctctgtatat tcctgattta aatccttttt taatgtctta
     6241 tccaaattaa atatctcttc tcactctcct tatctctaat tgataatata aatatattac
     6301 aaaaaaatat gtatgtcaaa actgaaaatg caaaaagttc accttataag taaactttta
     6361 tttagc
//
LOCUS       DBGJFU010000173         6161 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.177, whole genome shotgun sequence.
ACCESSION   DBGJFU010000173 DBGJFU010000000
VERSION     DBGJFU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.177"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            218..1585
                     /locus_tag="ACOIAS_003459"
     CDS             218..1585
                     /locus_tag="ACOIAS_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGZ2703127.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1610..2218
                     /locus_tag="ACOIAS_003460"
     CDS             1610..2218
                     /locus_tag="ACOIAS_003460"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HGZ2703128.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            2266..2637
                     /locus_tag="ACOIAS_003461"
     CDS             2266..2637
                     /locus_tag="ACOIAS_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGZ2703129.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2816..3154
                     /locus_tag="ACOIAS_003462"
     CDS             2816..3154
                     /locus_tag="ACOIAS_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGZ2703130.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3620..4123
                     /locus_tag="ACOIAS_003463"
     CDS             3620..4123
                     /locus_tag="ACOIAS_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HGZ2703131.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            4170..5129
                     /gene="moaA"
                     /locus_tag="ACOIAS_003464"
     CDS             4170..5129
                     /gene="moaA"
                     /locus_tag="ACOIAS_003464"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGZ2703132.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5133..5603
                     /gene="moaC"
                     /locus_tag="ACOIAS_003465"
     CDS             5133..5603
                     /gene="moaC"
                     /locus_tag="ACOIAS_003465"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGZ2703133.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5639..6070
                     /locus_tag="ACOIAS_003466"
     CDS             5639..6070
                     /locus_tag="ACOIAS_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HGZ2703134.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2611 a          497 c         1118 g         1935 t
ORIGIN      
        1 tcatttagtt taataactct catttcatat gtaaagttgg taaaaaaatt aaaattaaaa
       61 gtaaaaaact aaataaatta atgttaattt gaaatatata tgaaggtttt tggagaatac
      121 ttacttatga taggaaaaaa tgttaaaata atagtgtaat caaatataaa ggactgaaat
      181 aattaacagt tattaaatat taggagtgta aaaatttatg ggattaaaag aatttgattt
      241 tattgaagag tctattgata tgttgaggac aatgtcacca acattagaaa ctatatcaga
      301 tgaaatagaa gagtattttg aaaatatctt ggatgaaaag aatcaagaat atataaatgt
      361 aacctctaga ataaaatcag aatcaagttt aagagaaaag ataattagaa atagatattt
      421 aaaaaaatat ggagaagcaa gtaatcttat tcataatgtt tctgacttaa taggcctaag
      481 aattgagtgt agatttattg aggatgaaaa caaaatatat agacttttga gaagatattt
      541 taataaaaca gatgacaaaa taaactatta taataaagag aataagaata ttaaattaaa
      601 actatctgag agacaaccca ataagcaaaa aaatggattt gaaatatata aaatagatgg
      661 agtttttgcg tatttagata gagaagtaaa atttgaactt caaattaaat ctcttgtaaa
      721 tgtgttttgg agtgaaatag aacataaaat aatttataaa aacaatacat atttacttgc
      781 tgataaattt ataaaggata tgatggactc tataaagaat aatcttacta tgatagataa
      841 tcagctttta agtatttata aaaactttca ttcagggaag tcttttaata tgaaagtaag
      901 taaaaaagaa attgaaaaat tatttgcgaa actagtctat gacgcttttt ctgaaaagat
      961 gaataaaagt ataggttttg ttgtagattt taaaaaacca tgtgagacta tattgaacta
     1021 ttcttttaat aaacaagata taagtgatga agttttaggt aattttatgc ttgatgagtt
     1081 tgcaagatta aatgaaattg taaacaagga tatagatttt aatgagcaaa tagaatttga
     1141 aagagaacca acatttgatg ataagttttg taaagaccta ggaaatcatt ttagaagtag
     1201 attaaacaca gaatttccat ggaacctatt ttttagaata ttatttgaaa tagagccata
     1261 taacaatacc aaagactttg aaaactttgt agagtttata aaagaaaatg tgctactaga
     1321 agaaaatagg gagcaattat tatgccaatt tgaagaagat agcaaactta taattgaaga
     1381 tatatatgat tgtatattta atagtataag tgagattgat agtgttgaaa ttttatataa
     1441 ctataatctt gaaaaaataa actttacatc aagtgaaatc ataaattgta tatgtagaga
     1501 atatgagtgt tatggtgaat acttagaaga aaaagaaaag cttatgaata catttaaaga
     1561 aaagattatt caaatctttg agtaataggt caatgctgaa gtggcgctaa tgcgccattt
     1621 ttcaaatata tatgaattaa gtaaaattat tttggaggag gcgatttgta tagagaaaac
     1681 aggtgtaatt ttggcagggg gaagaaattc tagaatgggt agagataaag cctttttaga
     1741 gttacatgat aagcttttta ttgaaattgc aatagaagca tttaaaaact ttgatgagtt
     1801 gataataata tctaataatg aagaattata ttctaaatac gatattaaag tttataatga
     1861 tatagtaaaa gatgttggtc caattggagg aatatatact gctcttgaat atgcaaagta
     1921 tgatatagtt acaattgcat gtgatatgcc atatctaaat aatcaaatag tcgaaagaat
     1981 agctaataaa atgaatgata aaagtgtgat ttcagtgaca aatggaaagt tacaaccatt
     2041 gtgttctgga tataaaaaga gtataataaa taaagtatct ttatgtataa gggaaaatga
     2101 tttaaagctc agaagcttta tagataaaat agataaaagt tatatatact ttgatgaaga
     2161 agatttattt ttaaatgtaa acactgttga tgagtatgaa aaattaacta aagtttaatt
     2221 agctatggtt gtaaaataga gtaggattta tatggaggag agtttatgaa tttacttaaa
     2281 tgcgattctt gggcagtaat attaaataat agcgataaag aaagtaaagc atataaaata
     2341 ttagatgaac ttaaaaggaa tatgtacaaa gttgttgcta tagatgaaga gaaaaaacct
     2401 attgaaggaa tagatgtgta tgaatgttta aaagatgttc cacacaatat tgatgttgta
     2461 gctattattg ataaacaatc taagatggat gtgattttag aagaagtaga gcttttggat
     2521 atacaaaata tgtggtttga aaaaggaagc tttagtgaaa tgatgattaa aaagactaaa
     2581 gatttaaaat taaatataga atataattta agtttatatg atgagttgac tagataaaat
     2641 tatatatctt aataataaaa atataattag ttaataaaac atattatttt atagtataga
     2701 ttggttaata aaagtacaga ttggttaatg aaacattaaa ttacaatatt tgttaaaaaa
     2761 attgtgagtt acatgatata atgtattaaa taggatacta aggagttgat atattatgtt
     2821 aatagtaact acagaaaaag tagaaggtaa aaagatatca aaggttttag gattagtgag
     2881 aggaagtaca ataagagcaa aacatgttgg aaaagatata ggagcaagtt ttaagaatct
     2941 tgttggagga gaacttactg gatataatga aatgctcact gaagcaagac aaattgccat
     3001 aggtagaatg gttgaagatg cagaagctaa aggtgcaaac gcagtaatag catttagact
     3061 gtcttcagct tcagttatgc aaggggcagc agaaatgctt gcttatggaa cagcagttgt
     3121 tttagaagat gataatagta ttcttgaaaa ataaaatata aatagttaaa aagaggcatc
     3181 ttttaaaaat tttatggatg ccttttttta tttgaaaaaa ctaatttgag aagaaatttt
     3241 aactattatg acaataaaat tagggtgata ttatataaat aagtaaaatt atattatata
     3301 atgtatggta tagactttaa aaattttatt tgtagagaaa aatatggaca atgttataat
     3361 aattatagaa attaaaagta cttacattca ttatgtatgt gacatgtata attagtttta
     3421 tatttaatac agttttgaag taatatcttc attttgagaa cgttggaata ataaaaaatg
     3481 tattattata ggtgatataa ataaaaaata aatataatat gttatattgc tgatagaaaa
     3541 gagaataaaa gaaaataggt tttattaaaa gagattttta tttataggac ttaatcaatg
     3601 tatagagggg tgagaacata tgtttaatgt tgcaattata acacttagtg ataaaggata
     3661 tgaaggaaaa agagaagata taacaggaaa aaagttaact gaatttgttg aaaatacagg
     3721 agcttataaa gttactgaat atgtacttat aaaagatgat aaggaaatgc taaaggaaaa
     3781 tattataaga ctttgtaata gtaataaaat agatttaatt cttacaaatg gaggtactgg
     3841 tttttcaaaa agagatataa ccccagaagc aactaaggaa gtgttggaaa aagaaatacc
     3901 aggtctgagc gaatatatga gaatgaaatc cacagaaatt actaaaaagg ccatactaag
     3961 taggggtgta agtggaataa gaaataattc tataataata aatttacctg gaagtcctaa
     4021 aggtgcagtt gaaaatctaa gttttataat tgatgtttta gaccatggca tagaagtttt
     4081 aagaggagaa gctacagaat gtgccactaa aaaaagtgaa tagtatataa tctagataga
     4141 taaaaaatag acaaagaggt agataaagta tgattgataa gtatggaaga aaaatagact
     4201 accttcgtat ttcacttaca gataagtgta atttaaggtg tgtttattgt atgccaccag
     4261 atgtaaaatt tgataaaaat tatataaatg aaaatctaag ctttgaagac tacaagttta
     4321 ttataaaggc tatggctgaa caaggtataa caaaaattag atttactgga ggagaacctc
     4381 ttttatatcc taaattaaat gagcttataa aatttacaaa agaagaatgt ggaataaata
     4441 acataggaat gaccactaat gctattgggc tttctgatag aatctttgag cttgaaaaat
     4501 gtggcttaaa taaagttaat ataagtttag attctttgaa ggaatataag tataaatccg
     4561 taacaagagg aggaaactta aaagatgtct taaaatcgat tgaatcttgt ttaaacttag
     4621 gtataaaagt aaagttaaat tgcgttgcta taagtgggtt taatgatgat gagcttaaag
     4681 attttatgtt tttaacagtt aaatcaccaa ttgatgtaag atttatagag cttatgccat
     4741 taggtgaggc taatagagtt tataagaagg gttattttaa tgtaaaagaa atgattgaaa
     4801 aaatatatgg tttatataaa gtcaaaaatg atgaaaaaag tacagcagaa tattatatgt
     4861 ttaaaggagc taaaggaaga ataggaatta taactccatt aagctgttca ttttgtaaca
     4921 cttgtaatcg tattagactt acttctagtg gtgcaataaa attatgtcta cattcaaaag
     4981 aagaaataga tataaaacca tttttacata aaccattgtt atttagagaa tctatgaaag
     5041 atataataaa acaaaaacca gaaaaacatc atttaataga aaataaatgt agtgatacaa
     5101 ataggcgtat gtatgaaatt ggaggataag taatgttaac acatataaat gaaaaagggt
     5161 atgccaagat ggtggacata agtgaaaaag atgatactaa aagaacggcc gtagctacag
     5221 ctacaattac tcttagtaag gaagcattag aaaaagtaaa aaatggtcag ataaaaaaag
     5281 gagacgtatt gtctgtagct caagttgcag gtataatggg agctaaaaat acatcttcaa
     5341 acataccaat gtgccatcag attaaattag ttggatgtga tttagaattt gatgtagatg
     5401 attctatgtc tgctattaag atatacgcaa cctgtaaatc tcttgggaaa actggagtag
     5461 aaatggaagc tttaaatgca tgttctttag cagcacttac aatttatgat atgtgtaaag
     5521 cagttgataa aaaaatggtt ataagtaata tccatcttgt agagaagact ggtggaaaat
     5581 caggagattt taagttcgag taaattaata atatattggg ttaaaatttg gaggaaatat
     5641 ggcgaaggta gtgtcaataa atataagtga gaaaaaaggt gtaattaaag ttcctgtaca
     5701 atgtgcagag ctaaaaataa atcatgggat agttggagat gcacatgcag gaaactggca
     5761 tcgtcagata agcttacttg gaaaagaaag catagataaa atgaaaatga aaggctttga
     5821 ccaattaaaa tatggagatt ttgctgaaaa tgtaactaca gaagggatag aagttttttc
     5881 cctgcctgtt ggtacaaagt taagaatagg gcaatgtgaa gttgaagtaa ctcaaatagg
     5941 gaaaaaatgt cacaatggat gcgaaattaa gaaattgaca ggtgactgtg tgatgccacg
     6001 agaaggaatt tttgtaaagg ttctaaaaga aggaattata aatgtgaatg attcaataga
     6061 tgtaatctaa tgaagtcact attaaaatat aattataagt aaatactatt aagatttaaa
     6121 tatatatttt aagagagaat taattttaat aaaatatttt a
//
LOCUS       DBGJFU010000174         6123 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.178, whole genome shotgun sequence.
ACCESSION   DBGJFU010000174 DBGJFU010000000
VERSION     DBGJFU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.178"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            91..252
                     /locus_tag="ACOIAS_003467"
     CDS             91..252
                     /locus_tag="ACOIAS_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HGZ2703135.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            593..1861
                     /locus_tag="ACOIAS_003468"
     CDS             593..1861
                     /locus_tag="ACOIAS_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGZ2703136.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            2056..2844
                     /locus_tag="ACOIAS_003469"
     CDS             2056..2844
                     /locus_tag="ACOIAS_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HGZ2703137.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            2844..3293
                     /locus_tag="ACOIAS_003470"
     CDS             2844..3293
                     /locus_tag="ACOIAS_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HGZ2703138.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            3297..5561
                     /locus_tag="ACOIAS_003471"
     CDS             3297..5561
                     /locus_tag="ACOIAS_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HGZ2703139.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVK
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            5701..6012
                     /locus_tag="ACOIAS_003472"
     CDS             5701..6012
                     /locus_tag="ACOIAS_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703140.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         2383 a          639 c         1149 g         1952 t
ORIGIN      
        1 gagatatttc ccttttttga ttaaaattat agaaatttga aataataagt aataaacaga
       61 aaaagtgata ttgttttgaa aggagaaact atgaaacaag ataaacaaaa tgaaaaacaa
      121 agtgagattt ctaagataaa acaaagcaat gataataaac ttgaaatagc tcaagaatta
      181 catgtaagta aaaatatagg gaatatgctt ggatatagaa tagataagaa aaaaatgaat
      241 agaccaagtt aatgaaaatg ttgaaaatgg tacattttaa aaaatatata taatgaaatg
      301 ttttattgtt aaatttaaac ttattttaat gggggaaaaa gtcgaaaaat atctaaaaag
      361 atatggatat aaaaaatatt aatatatata tactataaac ttgacaaaat caacctttta
      421 ataactagca aaaaatagca aaattatata agcgttattt aattgtcaaa aatgaaagct
      481 aaattgcatg tcctaaaaaa aatgattgat aacatttatt tgtgtgctat aattatatag
      541 taaaacaata agtatattgc ataatgtatg caaatcggga ggagagacgg acatggaagc
      601 atctatacac acaattgcca gcaataattt attgataatt tttgctattg taagtataac
      661 tggaataatt tgtagtaagc ttagtgaaat aataaaggtt ccagatgtag tgttatattt
      721 acttgtaggt attttaatag gaccatcatt tttaaaattt atagatattc gtggatttca
      781 aatagaaaat caactaatac ttacatttgg ttctgcattt attttatatt taggtggaaa
      841 agaaataagt ttaaaagtat tgagaaatgt gaagatatct gtattcttat tagctacttt
      901 aggagttgtg atatctgctt ttataatgca acaagtaata ggatttactt ttgggataag
      961 tgcaatgact gctttactag caggaactat aatagcatca acagacccag ccactcttgt
     1021 acctatattt aatcaggtaa agataaagga tagggtaaaa caaactgtaa taagtgaatc
     1081 agcttttaat gatgcaacag gagctatact aacatcagca gtgttggctg taattttgtc
     1141 aaataaattt tcattaggtg aaaatattta tgaactgggg acaatggtta ttgtcggagt
     1201 tttagttggt ttaattacag gaatactgct tttgaaatta gttaatgata aaccttatgg
     1261 aatatttaaa gattttgcac caataatatc tgttatttca gttgtagttg catatgaatt
     1321 atctacaaaa ttaggtggaa gtggatatat gtcttgcttt attgtaggta taataactgg
     1381 taacaagaaa aactttaaaa tttggttaag tcaaaaatct tatgatgcag atttttatgt
     1441 tgctgaaact ttaggtacta tttgtcgtat ggcaatattt ataatattgg gtagccaagt
     1501 taatttggct cagttaagta aatattttat gccatctgta attacagtat tagtattaat
     1561 gtttatagca agaccacttt gcgttttagt ttgtacactt gtagacaggg aagcaaaatg
     1621 gagtaaaagt gaaatattat ttatgatgtg ggtaagggaa acaggcgtaa taccagcagc
     1681 actttgtggt attatttcag caatgaaagt tcctggatac gaagtgattt catctgtagt
     1741 atttatgact atactaatta cattagtaat acaaggaagt actacaaaat tagtagctaa
     1801 aaaacttgga ttattagaag aagaagtgat aaatataagt gaaaaagtat caacttttta
     1861 aagttttgct ttaaaatagt acctgttaat ggctatttta atattgtcta atgatgatta
     1921 ttgaaatagt cattttttta tgtataagta atttacagaa tttacatatt agacatattt
     1981 attatcaaaa tttgttgaag ctatgatata attagttgta tttaaaataa gaatagcaaa
     2041 taagggagga aatttatgtt tacggttatg gatattgtac agccagatac agtagaagaa
     2101 gcgtactcaa ttttaaataa aagaaaaact aaccaagtaa ttggtggaag tgctttttta
     2161 agaatgggga aaaaaagaat aggaacagga atagaattat ctaaattaaa tttagattat
     2221 ataaaagagt atgaagatta cgttgagatt ggttctatga ctacttttag aacattagaa
     2281 acaagttcca taataaaaaa taattttgga agaataatag aagattcagt taaaaatata
     2341 ataggagttc agtttagaaa tgtagtaaca gttggtgcaa cagtattttc taaatatggt
     2401 ttttctgact tgatagtagc attactttca ttagatacag aagtagaatt atataatatt
     2461 gggagaatta gtcttgaaga gtttctaaat agagattatg agaaagattt acttattaag
     2521 atatacataa agaagacaaa taagaatgct tcttataaat cacttagaaa tgcaaagagt
     2581 gattatccaa ttctaaatgt atctgtatca aaacatatgg aacaatttaa gttatgtgta
     2641 ggagcaagac cgcagaaagc tacgattgca aaacaagcta gtgaatttct atctaacaat
     2701 gaaatcaatg aaattaatat agataaagca gtagaaatag catcagaaga actaactttt
     2761 ggttctaaca tgagagcatc tagggagtat agaaaagcta tgagtaaagt acttttaaaa
     2821 agagctataa tggaggttat ttaatgttag taaagattaa tataaatggg aagaaaaaaa
     2881 gtgttgatat agaaccagaa gaatatctgg ttgatacttt aagaaaagtt ggtaatttaa
     2941 gtgtgaaaag aggttgtgac acaggctgtt gtggtttatg tacagtttta attgacaaga
     3001 aacctacatt atcctgtgca acactgacat tgagagcttt agataaagaa ataactacaa
     3061 ttgaaggttt agaaaatgaa gttaaagaat ttagtgaagt tttggtaaaa gaaggagctg
     3121 agcaatgtgg tttctgttct cctggcttta tacttactgt acttgcaatg aaggatgagc
     3181 ttgaaaatcc tacagatgat gaaataaagc attatcttac aggaaatcta tgtagatgta
     3241 cagggtatat gggacagtta agagctatca aaacttatct ggaggtaaaa tagaacatga
     3301 atatagttgg aaaaggaatc aagaaaatag atggaatgag tatcacaaca ggaaagcctg
     3361 tatatacaga tgatttatca gcaaaagatg ctttggtggt aaagatactt agaagtcctc
     3421 acgcttatgc aaaaataaaa gatatagata catcaaaggc agaaatggtt gatggtgttg
     3481 aatgtgtttt aacatataaa gatgtaccaa aatctcgatt tacacttgct ggtcaaacat
     3541 atccagagcc atctccatat gatagattaa ttcttgaaga tgttgtaaga tatgtaggtg
     3601 acgaagtagc tatagtggca gctatagatg aaaaaactgc aattaaagca atgaatctta
     3661 tcaaagtaaa gtatgaagta cttacacctg ttttggattt tgaagaggca ataggaaata
     3721 aaactatggt acatgatgaa gatagacatt gtaactttga tataggtatg caaagagaaa
     3781 aaaatatagt atcccatcat gattatacaa aaggtgatgt agaaaaagta ttaagtaatt
     3841 gtgatgtagt gattgaagaa acttattata ctcaagctca agcacatgca atgatggaga
     3901 cgtatagggc atacaattat ttagaccata tgggaagatt ggttgtagta agttcaactc
     3961 aaataccatt tcatgtaaaa agacatttat ctcgtgcact agaaatacca gctagcaaaa
     4021 ttagggttat aaaacctaga attggtggcg gatttggtgg taaacaaact gcatgtgttg
     4081 agattttttc tgcaattgtt actttaaaaa cagggaaacc agctaaaata atttatgata
     4141 gaaaagaaac ttttaattgt tcaacaagta gacatgcaat gagattaaaa gttagactag
     4201 gtgctacaaa tgatggcata ataaaggcaa tagatataaa agcactttct gatacaggag
     4261 catatggaga gcatgcttct acaactattg gtttggttgg agaaaaaact ttaccaatgt
     4321 ataataaagt tgaagcaaca agatttattg cagatgtggt atatactaat aagatgccag
     4381 caggagcact tagagggtat ggagcatcac aaggatgttt tgctgttgaa tcaactataa
     4441 atatgcttgc taataaatta aaaatggacc caacagattt aaggttaaaa aatgtggtaa
     4501 cagaaggtga aactacattt gcatatggta aaactttaaa tagtgtcgat ttaataaaat
     4561 gtataaataa aggcaaagaa cttattggat ggaatgaaaa atatccttgt aaacatcttg
     4621 ataatggaaa aattagaagt gtgggtatgg caattactat gcaaggttct ggtatacaag
     4681 gtcttgataa gtcttcagct gaaataaaat tgaatgactg tggagattat actcttatga
     4741 taggttctac tgatatgggt acaggaagtg atacgatttt agctcaaatg gctgctgagg
     4801 ttcttgatac aagcattgaa aatataacag ttatatcagc tgatactgac ttagttccat
     4861 atgacccagg ttcatatgct tcaagtacta cttatgtaac aggtatggca gtagttaagg
     4921 cagctcaaga cttaagagaa aaaataattt ctgctggagc aactaaacta ggtgttgaga
     4981 aggatgaagt aaaatttgat ggtataaaat taactagtaa aaaaggtagc actttattat
     5041 tcgatatagc agttgattct acaattggtc cagatactaa tcaattaata ggtcttgctt
     5101 ctcatggtag cccagtatct cctcctcctt ttatagcagg ttttgcagaa actgaaattg
     5161 acacagaaac tggaaaagtt gatttagtaa attatgtagc tgtagtagat tgtggaacgg
     5221 ttataaataa aaatctagct aagatacaag ttgaaggtgg aattgttcaa gggattggaa
     5281 tggcattatt tgaagaagtt agatacacag aaaaaggaag aatggacaca aatacattta
     5341 tgcaatacaa gattccagct agatgtgata ttggaaatgt taaagtagat tttgttgaaa
     5401 cttatgaacc aactggacca tttggtgcta aatctgttgg agaagttgtt ataaatacac
     5461 ctttaccagc aatacaagaa gctgtgttta atggaattgg tgtaagaata aatacactcc
     5521 ctattacacc tgaaaaagtt tttatggaaa tgaataaata attatttatg aaaatttaga
     5581 ctagttttac atgttaaaca accagttatg tcatacctgt tgaaagatat ttaataagta
     5641 gttattataa tattatgaat tttacttata aaatacaaaa ttaaataagg ggatgatgtt
     5701 ttggctggta atctaaataa tatgagagca gtaaataatt ttagaggaga taagaacatt
     5761 ttagaatgtt tagtcagctt tgagggtcgt tcaataagtc agagaaaagt aagggtattt
     5821 tttaaagaaa aacaaaatca aatagaaatt gattttgcag aagaggaaat ttctaaattg
     5881 gttgaaaatg ttgttttaaa tacatcatat caagaaatgt tatatgatga aatagagaaa
     5941 caactggaaa ttgattgtat aggtacttgg atgatattat ctaaattaaa agatggtagt
     6001 agagttcact gattttaaaa taaattatta gagcctaatt ttaaggctct tttttgctgt
     6061 ttttaattaa caatagaaaa cttatcttta gtttgtatat aattttaata tcatatattc
     6121 tat
//
LOCUS       DBGJFU010000175         6090 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.179, whole genome shotgun sequence.
ACCESSION   DBGJFU010000175 DBGJFU010000000
VERSION     DBGJFU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.179"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..678
                     /locus_tag="ACOIAS_003473"
     CDS             <1..678
                     /locus_tag="ACOIAS_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2703141.1"
                     /translation="ERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYPNNVDIIFM
                     DIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPLKYSLFKE
                     AISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIYTFKSSMK
                     EIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIKLLREKLNS
                     CLVDVII"
     gene            951..2723
                     /locus_tag="ACOIAS_003474"
     CDS             951..2723
                     /locus_tag="ACOIAS_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGZ2703142.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            2745..4166
                     /gene="ccpM"
                     /locus_tag="ACOIAS_003475"
     CDS             2745..4166
                     /gene="ccpM"
                     /locus_tag="ACOIAS_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030152 - bacteriocin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HGZ2703143.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            4195..5265
                     /locus_tag="ACOIAS_003476"
     CDS             4195..5265
                     /locus_tag="ACOIAS_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HGZ2703144.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            5281..5532
                     /locus_tag="ACOIAS_003477"
     CDS             5281..5532
                     /locus_tag="ACOIAS_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030152 - bacteriocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HGZ2703145.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            5583..5786
                     /locus_tag="ACOIAS_003478"
     CDS             5583..5786
                     /locus_tag="ACOIAS_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703146.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2511 a          518 c          941 g         2120 t
ORIGIN      
        1 gagagggtaa ttctaaatga ttatataaat accttttttt ataattacaa ttttgaatac
       61 aatatcaaag agtttgaaaa tggaggcgat ttattaaaaa agtatcctaa taatgttgat
      121 atcatattta tggatataga cttaaatgat gaaaatggat ttaatgtttc aaaagaaatc
      181 aaaaaatttg atgaaagtgt aagaattata tttgtatcct catcaaataa atacataaag
      241 aaaggttatg agataggagt ttatagatat ttagagaagc ccttaaaata ctctctattt
      301 aaagaagcaa tatcatcata tttaaaagaa tgctacaaaa atgaagctat tttatctata
      361 aaatgtaaat gcaagatata tttcataaag gtagatgata ttttatattt agaagtttta
      421 aaaaatgatt taactataca tactgaaaaa ggaatatata cttttaagag tagtatgaaa
      481 gaaatagaat caaatctaga agaacataat ttttttagat gtcataaaag ctaccttata
      541 aatttaatga aggtaaatat gctagatata gaaaataata ttgtatttat aaatcaaaat
      601 tcaataattg taagtcgtta taagataaaa ttattgagag aaaagttaaa tagttgtttg
      661 gtagatgtaa taatttgagg taaatttttg cctttatatt ctattctaat agaatatatc
      721 tattgtaaaa tatgaaaaaa tttataataa aaactaatta tagttaaaaa tatacctttc
      781 atcaataaca gattgaatat attaagattt tattttacaa ttctattaac aattttattt
      841 taaagattta atttaaaatt taaatatgta ttatacataa taaaataatg ttatattata
      901 ttaattaata aaatatttta tttcaatttt attaagtatg gaggtgtatt gtggaaaatg
      961 gtaaatttca attttttaaa ggtgtaagaa attgttttaa tatgatttat ctacatgata
     1021 aaagatattt atttatctct atgtttatgg caatttttca ggggattgtt ccatctattt
     1081 ctttgttagt aagtcaaaat atcattaata atatgcaaac attttcgaaa agtgtaaaag
     1141 aaatattagt tttgttaatt atgtatttaa tcataaatat aattccttca atttttcaaa
     1201 gtttttatgg atattataga ataaagttta ataaaaattt tgaaaaacat attgaaacaa
     1261 tgatgttaga gaaatcttta actttgagtt taaaagattt tgaggatgat aatacttatg
     1321 acatgataaa tagagctcaa tctcaaaaag gatttaatat tactatacta tctgaaaacc
     1381 ttatttcaat aataagagaa tcagtatcta cactttctct tattgttgta ttatcacaat
     1441 ttcaactttg gctaataata gttgttattg ttattcctgt attgaaatca ttatattcca
     1501 ttaaattagg aaaggaacaa tatgatgtta gtgtaaacag aacttggaaa gaaagaaagg
     1561 cctggtacat agattatttg ttgtcaaaag gttatgctta caaagagatt atggtattta
     1621 gaattggttc taaacttatt aatcattata aaaaattaaa agaggatatt atatctcaag
     1681 atatgaatat agctaaaaag aattttattg ctacgctatt atgtacagtt ttagattcaa
     1741 ttgtatctgc aggaattata ggatttataa taattcaagg tattttaaag aagattttaa
     1801 taggagatgc gactacatat atacaagcag ttcaatctat aaaggataat gttgaatcaa
     1861 tattttcgtt atttagttca gtaatacaag atctttttta tacagattta ctgtttaaat
     1921 ttctagatta taactgtaaa gaagactcaa ttgaacatat aaaaatattt gaaaaaatag
     1981 gggatataaa taaaatagag ttaataaata tttcatataa atacaatcat tcatcagagt
     2041 attctttaaa aaatattaat ttaactttaa ataaaaatga tcccattgct atactaggaa
     2101 aaaatggttc aggtaaaacg acacttttaa aaatattatt aggtttttat gatgattatg
     2161 aaggtgacat cttgataaat ggaaaaaatt taaaggatat tgaaaaagat gaatacatta
     2221 gaaaagtaag ttgtatgttt caagattata ttaaatatga ggctacatta aaagaaaatg
     2281 tcgccatggg agatgtatat aagagtaact tggaagaaaa agttaaaagt aaacttaaag
     2341 aaactaaatt gaaaccaagt atttatgagg aagatggatt agaaactatt ttaggaacct
     2401 ggtttggtaa gaagcaactt tctatgggtg aatggcaaaa agtagcaata tctagaactc
     2461 ttatgaaaga tgctgatatg tacatttttg atgaaccaga ttcatcttta gattctgaag
     2521 cgggaaatga actcctttct ttatataaaa aaacatttga aaataagatt ggaatattta
     2581 taacgcacca tatccaacat gcaaaaaaga taactaacaa tattgtagtt cttgaaaatg
     2641 gaaagttgtt agaaagtgga agacatgatg aacttatgga caaaaagggt gtttatttta
     2701 atttattttg taactgcaaa taaatattaa agggaggtta attaatgaat caaaaaccat
     2761 ttatacacac atttaaagct ggaaaatcat attttattta tgatgttaat acggataaga
     2821 ttttaaaggt aaatgctgct gtttataatt atctaaataa aattgagcat aatttagaaa
     2881 aagaaagtga ctggaacata gaaatagaag acgaaattaa taccttgatt aattatggat
     2941 ttctaaaaaa taaaagggta agtaaaacaa tgcaccctga aactaagtat ataaaatatc
     3001 atatagaaaa taatctatcc tctattattt tacaagtaac ccaaaattgt aatttacgtt
     3061 gtgactattg catttattct ggttcataca gcaacagagt tcatacaaat aaaagaatga
     3121 atatagatgt agcaaaaaga agtattgact atttgattaa gaattctagt caattaaatg
     3181 aagttagtgt tggcttttat ggaggagaac ccttacttga gtttgatttg attaagcaga
     3241 tagtagaata tgtaaaagta gttggagaag gaaaggaaat atactttaat ctaactacaa
     3301 atgcaacttt atttaatgag gaaataatag aattctttgt aaaaaataat ataacaacta
     3361 tgattagttt agatggagga aaagaaattc atgataaaag tagaaaattt gcaaatacag
     3421 gagaaggatc tcatgatgtt gtattaaaaa atgtacgtta cataaaaaat aaatatccag
     3481 aatattataa aaagaatatt acatttaata cagtgataaa ttcagaaaat gattacattt
     3541 ctatagatga ttttatatct aaaaatgaga ctttaaaaga ttcaaacttt ttagcttcca
     3601 ctcttaataa taactattct gataaagaaa tagtttcatc agatgaatat atattaggta
     3661 agagttatga tagattcttg actttactca acaaattaaa atttataaaa gatgagagta
     3721 tgtcaccttt atcaagaata gaaagagcct tattagatga ttttagatat gagaaaaata
     3781 ttgaaggtag attagagtta ccagatgtgt ggcatcatgg tggaccatgt ataccaggag
     3841 cttttagaat atttgtaact acagatggag aattttttcc atgtgagaga gtaagtgaaa
     3901 gttctaaaat gggtatgatt ggaaatttag atagtggagt agatatagaa aaggctgaaa
     3961 aacttttaaa tataggaaaa ttatatgaag agcaatgttc tgattgttgg gcctatagtt
     4021 attgtgatat atgtatagct aagattgatg atactaaatg taatgaaaat gaaataaaac
     4081 cacatgattg tgacagaatt agacattcta cagaaaataa tatgaaagat tactgcgttt
     4141 tgaaatctct aggatatcaa tattaaattt tctaatgaaa ggaaagatgg gattatggaa
     4201 aaagtactta tatatccatt taattataat ttttttacta ttttaaatca ttgcaataaa
     4261 atttcagata tagaaattaa ttcactggtt agtccttctg gctgggggtt atgcaataag
     4321 gagtataatg ttaataatat tattactaaa gttacaaatg attttcaaag tgaacttaaa
     4381 aagaattctt tggtatggtt tgttgattca aaagaaaatc tagacttaga aaaatttata
     4441 attcctaaat taaaagtagc tctaaaagaa ggtaaaaaga tattatatac acgtgatgat
     4501 cttaataaaa tatctaggta tatatctcat tatgaattca aaaagattaa tgaatggaat
     4561 ttagaaaaat atgaaataag agatatagag ggtatatcac agataaacac accaataata
     4621 tttatttgtg gtctttatgg agggttagat acttttgatg taaatataag gataggagaa
     4681 gaactaagaa aaagggggtt ttcaatatta caatttggcc ctaaaaaaga gagctattta
     4741 tttaaaatga ttcctatacc agagtttatg tttgatgatt cgataagtag taagacaaag
     4801 attattaatt ttaaccattt tataaaagat aaagaggtag aggcaaagcc agatttaata
     4861 attatagaag ttccaggaga attattccct ttctctagaa aaatggttgg taattttgga
     4921 attagagctt ttgaaatttc taatgcagta ataaatgatt gtggtatatt atgtattcca
     4981 tcaagtatat atgatagaga atattgtgaa gaacttaaat attttattaa aggaagacat
     5041 ggtataaata tagactattt tagtatagag aaaaaagtta tagatacata tgagacagag
     5101 gtgaagcaaa aattttcata tatgtcatta gatgaaaact tttttgttga agatatagaa
     5161 gattatgaaa aaacattttt tatacatgat aataaacaat tactagaatt agtggacaat
     5221 atagtgaatc aattaaaaga gtatgctaaa gtaatatcta tttagaattg gaggttaatt
     5281 atgaattaca ctgaaaagga attactacta gagaatatat tttttgatgt gactggaatt
     5341 aattttaata aaaataacaa tttaaaaaaa gaattatttt tttcaccgtc tttacatata
     5401 cagccaagag aattactttt agtattttgc gaggtaatag aaaactttaa tataaaaatt
     5461 ccagaggaaa aacttttgaa taggcaattt acgagttttg ataatgttct aaaaataatc
     5521 aatatggtat aggttaataa aggtataaag taagtgatct tatagaaaag gggaggatat
     5581 gaatgagttt tggaaaaaga atagaaatga ataatatagg tgttgagaca agaggtgttt
     5641 gtactattat gtgtccttat acttgtaaga tgatggcatc ttcatcttgt aactgcaaga
     5701 ataatgatta taatgcaaga ggaggatcat ataatacata ttataataaa ttgatacatg
     5761 gccaaagaac tcctgatgga atgtaaatat ttcagtaata agtatactat taaattgatt
     5821 tagcatattt gattaaattt atctttctta ctttatttcc ttttattata aaaatatgca
     5881 gttgattata attaagagtg tgattttata ataactgcat aaatagtatc tgttatataa
     5941 atcacctctt caattattat agtggtaaat atagtgatta ggggtgattt ttacaattta
     6001 aaagtataaa ttaattaaaa gttattataa taaattatac ttatttaaat agtaagaatt
     6061 aaaagtaaag tttttaaata tatttgtaaa
//
LOCUS       DBGJFU010000176         6032 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.180, whole genome shotgun sequence.
ACCESSION   DBGJFU010000176 DBGJFU010000000
VERSION     DBGJFU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.180"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(5..412)
                     /locus_tag="ACOIAS_003479"
     CDS             complement(5..412)
                     /locus_tag="ACOIAS_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HGZ2703147.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(886..1770)
                     /locus_tag="ACOIAS_003480"
     CDS             complement(886..1770)
                     /locus_tag="ACOIAS_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGZ2703148.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2061..2537)
                     /locus_tag="ACOIAS_003481"
     CDS             complement(2061..2537)
                     /locus_tag="ACOIAS_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HGZ2703149.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            2803..3978
                     /locus_tag="ACOIAS_003482"
     CDS             2803..3978
                     /locus_tag="ACOIAS_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HGZ2703150.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4059..4862)
                     /gene="proC"
                     /locus_tag="ACOIAS_003483"
     CDS             complement(4059..4862)
                     /gene="proC"
                     /locus_tag="ACOIAS_003483"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGZ2703151.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(4902..5492)
                     /locus_tag="ACOIAS_003484"
     CDS             complement(4902..5492)
                     /locus_tag="ACOIAS_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGZ2703152.1"
                     /translation="MVLNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLT
                     SVATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLK
                     EKTMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYN
                     SEKNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2019 a         1009 c          664 g         2340 t
ORIGIN      
        1 gtagctattt tttaatcaat attttagtaa attttacggt aagtaaaaac ctctctcctt
       61 caaatcctat agcaaatata cttacaagtt gctttgagaa aataactcct aatatagcta
      121 caactatgca tataccaact attatattta aaacattgtt taaaaacttt agtgcttgct
      181 tttcaccttg tttactactt atatcttgat acattggaat aaaagtagta actatagcag
      241 tacctattgc tgcaaatata atatttggta tattcatagc tgttaaataa ctctctgtat
      301 ataagcctgt accataaatt gatgataata ctaattctct acccattcca agaatcttag
      361 agattatagt aactatcatc aaataaaaag ttgctttagc taccttgctc attttatcac
      421 tccacttcta acatacctct ttttaatcaa gtaaaattta gctcattaga tataattcta
      481 tcaactaatt tgccttgctt agccctatca aaatataatt aacacctatt tcttaatata
      541 tcacaaacta cattattctt catatttttt tatttctcca ataagtttta catttttcct
      601 atataaattg tatagcttca attatattca aagaaatgga gttgctaccc gataacttta
      661 aaagtaaaaa acagatttta ttatcttttt aaactatatt ctaacatatt ttgtccatat
      721 tgagaaattt tactaaacta ttatgtactt tattaaataa ataaaataac agcacaccta
      781 taaaatacaa gtgtgctgtt cataaacaga ttttataaaa aaataattcc taagtagaga
      841 ttaacatgct ttctcatact ctcatatata atgtgtctaa aaatattaat aaaaatgttt
      901 taattgtttt aacttacttg gatgtcttaa ttttcttatt gctttagctt ctatctgtct
      961 tattcgttct ctggtaactc caaatacctt tccaatctgt tctagtgttt tagggtcatc
     1021 atcatcaatt ccaaatctca ttcttaaaac ttgctgttct tgctctccaa gacctgtaag
     1081 tagttcttct atcttttctt ttaaattatg ctgttcaact tcgtgtatta caacatcttt
     1141 aaaatcagca tctgatggta taaattcaac taaactacta tcctcatctt cttttaatgg
     1201 agtatccaaa gatacaacat ttttatctct tcttaataat tccaacactt tgtcaacttc
     1261 taagccacat gcatctgcaa tctcatctat agttggctct cttaataata catttaaaag
     1321 ttcttgtttc tttttcttta caaaatttat tctctgatga agatgtatag gtatcctaat
     1381 agtattttcg caatcgtcga tatatcttgt tatactttgt tttatccacc aagttgcata
     1441 agtgctaaat ttgtatcctt ttttataatc gtacttttct acagctttta taagtcctat
     1501 atttcctgct tgaattaaat ccaacatatc aatactatct cttttatatc tctttgctat
     1561 gctcactacc aacctataat ttgaatttat aaatttttct tttgctactg aagatgagtt
     1621 gattatttcc ttagccagct ctacctcttc tcctgctgac aacattttgt attcctctat
     1681 ttcctttaaa tacattttca tcgcattcga tacatttgat gaacccacac aaatataatt
     1741 ttcataatta ttttcttttt tagtatccat aatcaaaccc cttctattca tagaatttgc
     1801 taaaacaaaa ataaataatt taaacctaca taatctacaa ttacataata tcccattctt
     1861 taaatgaatt gttttgtata ttatgtatac aaatttataa aaactatacc tataatatta
     1921 ttctacttct aacagcaata tcctccttat atatataatt tattttatta tatgttgtta
     1981 tattcttagt tttttgaatg atatatgtct aaaaaaagac tgtttactaa acagtctttt
     2041 tttatatata tgctcaatat ttaattttca tgatgatgat tacatccaca tccacaattt
     2101 tcatcatggt gatgatgttc ttctaaatct tttacctgtt gttccatttc atgaaactct
     2161 tttaccatta tatcataagt tggaagagct gcctcagtat cgtattcttc ccctctccaa
     2221 tcagcgtaca tcatagcatc tccattagtt aaaaccaatc tacctgacat agttatatta
     2281 tccacttctt ctaaaaattc tatttgtttt tgtaaaactt ctccttctaa ctccacatct
     2341 tcatctaaac attgcactat cattacaggt gcataataat cttcttcatc tttaacattt
     2401 acaacaatat ctaaaattcc ttctgcacct tcttcaaatt ctgccaactc aacatttgta
     2461 tcaatcatat cttgaggtac taataaatct tctattaaat attttattac tctatctttt
     2521 actaattctt ttgacattta tttttctcct tccgattcta taacattatc ataatcattt
     2581 ctatattata gtatactaca ttaattgttt tttcaatctc ttacaaaaat tattttttat
     2641 tatcttcctt atattccctt ttttattaca aagttgtaat cgcattgatt taaaaaatag
     2701 tttatattta gtataataga aatgtagcac atatttttat tcaatatatt aataaaaaaa
     2761 tatattgaaa atgtaatgta tgttaaatct aggaggaata ttatgaagtt taaaaaacaa
     2821 attagttcta ttgttgtgtc tactatgctt gtacttggga gtatgaacct ttcttatgca
     2881 gacggtacaa atgtagttac acttggagct aacttatcag agtctcaaaa gcagcaaatg
     2941 cttaattact ttggagtaaa aaaagaccaa gtcttagttt tagatgtaac aaatgcagaa
     3001 gaaagacagt atttacaagg tgttgcaaca gagggtcaac ttggaaaagt tactatctct
     3061 tgctcatacg ttgaacctac taaaaaagga aatggaatta atgttaaaac agcaaactta
     3121 acttgggtaa ctagttcaat gattgcttca acccttacaa cagctggtct tgaagatgca
     3181 aatgttattg ctgctgctcc atatcctgta agtggtactg gagcattgac aggaatcatg
     3241 aaagcatttg aggatgcttc aggaaagaaa cttgatgaaa ctaagaaaga aattgcttct
     3301 gaagaattgg tagttacagg tgacttagga gatgatatag ggcaagaaaa agcaacaggt
     3361 gtaatgaatg atataaaaac agaaattgta aaaaatggta caaaagatac caatcaaata
     3421 gcaaatacaa taaataatgt tgtaaataat tacaatatta ctattacacc agaacaaaaa
     3481 gaacaaatta aaggtgtaat gaaaaaaata gctgaccaaa attacgatta caatagcatg
     3541 aaaaatacac tagataatgt atcagataat gtaaatgaac aattaaagaa attaggtgaa
     3601 agtgttaagg gttctggtat actagatact attggtggat ggtttagtgg aataggagac
     3661 tggttctctg gtttattctc aggcggagat aaaaaagatt tgggaatctt aaataatact
     3721 aatgatgagt ctcttggctc tgatgctgta ataaattcta cagaaggtgt tcaaataaat
     3781 ccacaaaata atactaattc taacaatgat agtgatacaa atagctcaaa aaatgatact
     3841 tcaagtaata atgaaaataa cgatagttct caaacaaatg aaaacaacac aacaaataat
     3901 tcagaaaatc aatcaaataa taatgactca gttggtttct ttgaaaaaat aaaaaattgg
     3961 ctttcaggct tattttagaa attaaacaaa taaaaagaat tgcctaaaat ggaaattttc
     4021 tataaaaggc aattctcttt atttacaata aatttttatt actcactcat ttttttagat
     4081 ttttctatac atgcacatat tgcttttatc acagaagctc taaaaccttc ttcttcaagt
     4141 actttaactg cttctatagt agttccacca ggtgaacata ccatatcttt taattcacca
     4201 ggatgttttc ctgtctcaag taccattttt gcagaaccca taacagcctg tgatgcaaac
     4261 ttatatgctt gtggtcttgg cattcctgca attactgctg catctgctat agcttctata
     4321 aataaaaata catatgctgg tgcagaacca cttgcaccaa ttacagcttc tataagatac
     4381 tctggtacta cctcagtatt tccaaaagaa ttaaatactt ctgtaactgc ttctaagtct
     4441 tctttgttta tatttttatt tatagaaaga gaagacatgg cttcatttac aagagctgga
     4501 gtatttggca tagttctaac tattttttta tcttctccta taatagactc tatatctttt
     4561 atagattttc ctgctgctat tgtaactata attttattgt catctataaa gtcttttatc
     4621 tcttccaaaa tatcatcata tatatctggt ttaacagcta caattactat atcagaggtt
     4681 ctagttactt ccttcgagtc ttttgtagta tttattccaa aatttgcatc tattctatca
     4741 agagttcctt gactatatgc tgatgctata accatattag gttcaactaa ttttgaattt
     4801 actattcccc ctatcatagc gcttgccata tttcctgcac ctatgaatcc aatttttttc
     4861 actttagtac ctccttattg actaatagta atcgttaaaa tttaagcttc tttaactgca
     4921 tcagactttt caaatggatt tggtatactt actaccaaaa ttttcactac cttattagta
     4981 tagttgaccc aattatgatt gttcttttca ctattatagt gtattgtgtc tcctggatac
     5041 atttctattt gttcatctcc caaaaacact gtaagagtac cctctaatac atacacaaat
     5101 tcctcacctt catgtacgta acaacaaatt tcctcactac tcttacttgg aagaatttca
     5161 attaatcttg gtagcattgt tttttccttt aaactagatg ataaatgata atgtataaat
     5221 gttgagtttt ctacatcaaa tacttctttt tcataacttc ttaatacagc tcttttatgc
     5281 tcttttggct tcataaaaaa gtaagttaat tctacatcta gtacacttgc tatttttcct
     5341 aaggaatctg ttgccacact tgttaatcct ctttcaagtt gtgataaaaa accaattgac
     5401 aagtttgttt tttcactcat atccttcagc gtcatttgct tttgagttct gagttgcttt
     5461 atttttgctc ctatgtcctt attcaaaacc atcaccccta aataatttta attttttcat
     5521 aatataaata caaaaatata aatataggta ctgaacctat ctttatttca tttaaaaaaa
     5581 tttttataag tattactata atttatcaat ctttttttgt ctacattttt acgattattt
     5641 acgatttatt tttcacatat gtatatttaa tcttgccaac taaagataca ttttttataa
     5701 gcatttactc tttatataat aaaacttaag ttgttcatga gtcttcttct ggtttaatta
     5761 ttaaaatttc aaattatatt attatttcta cacattatta catcctttag tacaattata
     5821 acataatttt acaatttaat gatatctatt caatgtcttt ttttactcta caacatattt
     5881 tattttagag tttaatacta tatatatttt ttatatacag ccttgtcatt ttttagattg
     5941 tcaagagtta ttgaagtatt ttatgtagat ttagatttta ttttctaatt tctcatttaa
     6001 caagaattgc ttatgataaa aaataatatc at
//
LOCUS       DBGJFU010000177         5767 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.181, whole genome shotgun sequence.
ACCESSION   DBGJFU010000177 DBGJFU010000000
VERSION     DBGJFU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.181"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(80..982)
                     /locus_tag="ACOIAS_003485"
     CDS             complement(80..982)
                     /locus_tag="ACOIAS_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2703153.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(1032..1493)
                     /locus_tag="ACOIAS_003486"
     CDS             complement(1032..1493)
                     /locus_tag="ACOIAS_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HGZ2703154.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(1545..2000)
                     /gene="def"
                     /locus_tag="ACOIAS_003487"
     CDS             complement(1545..2000)
                     /gene="def"
                     /locus_tag="ACOIAS_003487"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGZ2703155.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(1972..2235)
                     /locus_tag="ACOIAS_003488"
     CDS             complement(1972..2235)
                     /locus_tag="ACOIAS_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2703156.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(2332..3048)
                     /locus_tag="ACOIAS_003489"
     CDS             complement(2332..3048)
                     /locus_tag="ACOIAS_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703157.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(3045..3728)
                     /locus_tag="ACOIAS_003490"
     CDS             complement(3045..3728)
                     /locus_tag="ACOIAS_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703158.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(3725..4570)
                     /locus_tag="ACOIAS_003491"
     CDS             complement(3725..4570)
                     /locus_tag="ACOIAS_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703159.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(4684..5274)
                     /locus_tag="ACOIAS_003492"
     CDS             complement(4684..5274)
                     /locus_tag="ACOIAS_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2703160.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            5563..>5767
                     /locus_tag="ACOIAS_003493"
     CDS             5563..>5767
                     /locus_tag="ACOIAS_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGZ2703161.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPL"
BASE COUNT         2040 a          903 c          551 g         2273 t
ORIGIN      
        1 ttaaatttta tatttgtcaa taaattttaa ttttaataaa ttttaatcat catagtaaaa
       61 aaaatttttt tatataaaac taatcaaaaa aagatatctg tacatctcct gagtttttta
      121 acttttcctt cattatttta gagtaactgc ttgatagaga atacttatat ctaagttcat
      181 taaccatttt atacaattga tttttatact ccctattaag tgaacctgca ctatatagta
      241 ttgataattt atcaaataat tcaggaaact cttttttaac aaagtcaaaa aaaacacctc
      301 tagttatacc ccttaagtat aaagtcccag gtaaaacata atgtacatta cattcttttg
      361 catgtctaaa tagcgaattt atattatcaa aatcatctgt aatatatggt ataattggca
      421 taatatgtag accaactgat gcgttagttt ttctaaattc ttttaacatt ttaaatcgtc
      481 tcattgaatc cacaccattt ggctcaataa gcctttgtgt atcttcattt actgtggtaa
      541 ttgtagatgc tacattgata tatgtaatcc tagatagctt atcaattaaa tcgtaatctc
      601 ttaaaattaa atctgacttt gtagaaataa tagctggtgt cttgtatttt attagcaggt
      661 ttaatatttc tggcataatt ttatagtcag cttctattgg ttgataacta tctgtaactc
      721 ctcctatatt gattacttct ttcttccatt tactgctttt aagttgtctc tcaagttttt
      781 ctactatatt tgtttttaca taaatctctt caaaataatt attggagttg atgtacttat
      841 gagagtaaat agcataacaa tatttacatc catgctcaca tccacgataa atatttaaat
      901 cccatttata tggcatactc ctttttaatt tattacaagc tatttcacaa tttattggaa
      961 tatattttat tttattatac aaataatcac ctctttatat acaataatac tataatgtta
     1021 atctatagta attaaaacat gatatttcca gaccacaatt cataatcttt actatattta
     1081 caaagtaata tattccattc atttattggt attcctccac atgttgcttt ttcttctaaa
     1141 ttatataagc attgctttaa atacatttta ttatgccaat catcatataa attttctaaa
     1201 tttaaactat gttcattaga tatatcattt gcaaaatttc tatttttcca ttttaatata
     1261 cttgtatatt taagttcatt aggtttataa tttcttttta acatttcatc atgtacaatt
     1321 tttgtataaa atttaaattc atctatacta taattcaata ctttattcac taatagatgg
     1381 tttggagtac cttttttatc tatagaacct tttatagcaa gtagctctct ccattgactt
     1441 actaattgat ttttaggtaa tacatcaatt aaatccttat gccataatct cataatttaa
     1501 ctccttacta taaaatattt tataaattca ataaccaatc tatcttacaa ttttacatgt
     1561 tcaataattt tatctgtaaa aactatacca tttaaatggt ctatctcatg acaaagacat
     1621 tttgccataa aacctgaacc tttaatttct attttctcac cgtattcgtt tagagcttgc
     1681 actgtaactt tttttggtct ttttaattta ccccatactt caggaaaact taaacatcct
     1741 tcaacaacaa tttgttcccc ttcttgttca ataatttcag gattaacaag cttaataagt
     1801 ccttctccta aatctataac aactaatcgc tttaatactc ctacttggca tgcagctaat
     1861 cctcctccat ttggagtatt gtacatggtc tctgccatgt catttaatat ttctcttatc
     1921 ttattatcta cgttttctac atacttactc ttttttctta aaatttcatc atcaaatgtt
     1981 cttatctttc ttatagccat aatattctcc cctacttatc tgtgctcttg attttctttc
     2041 actttcttca aataaaaata cactttcaat tggttcatta aaaattcttg aaatatcata
     2101 tgccaaccat attgaaggtt catttttacc agtttctata gcatttatag cctgtctaga
     2161 tgttccaact aatctcccta gttgttcttg tgtcaatcct aatttttctc ttaatatctt
     2221 tattttattt ttcatatttg ctccattcat gtaaggttaa ctttacatct taaatgtaac
     2281 atttttcaac attactgtca agtcaacctt acatttatat attaaatttt cttatttaac
     2341 tattttttta ctaaactttt taaataataa taaaatccat attaatgata taacaattga
     2401 aataaacata tagacataat tatcttcctt aaatgccttc gatatccaaa aagttggtaa
     2461 gaagaacaga atatattgtg ctttatttaa aataaaaaat ggtgctaaaa tacccatcat
     2521 aaaaacacct gagaatttcg taattaccat tccttctagt ttatttgatg ataaagaaat
     2581 cactaatatt gatataataa ctccttgtaa taaaactaat aaagaaattg ataagtttaa
     2641 caagagagaa attttagtta gagaaaaaaa gataagtaga atcatagtaa taataaatgc
     2701 taagatacct ggtattccaa atcgtgaaat aagataacca gttttaccaa gcggagttac
     2761 tattaaatat cttgaaatgc tgtcatcaat ttctcccaat atgaccattg tagaagcaaa
     2821 aaagaacata aaaggtgtaa taaaagctaa aagtaaatca aacatcaaga aatacggctc
     2881 taaatttaat tgataataaa acttgttaag tgatatattc tgtattaatg gtattccaaa
     2941 ttttataaat actccacata atattggaga aacacatact ataaaaagca tagcatctct
     3001 cttaatttgt tcaaactctt gtttaaacgc atttataatc tttatcatag ttttactcca
     3061 cctacctttt tccaactgct acaaataaaa taataagtta taaaataaat tattattata
     3121 attgatatta aaattaaaat attaatcatt ataaaatttt tatctccact aataagagaa
     3181 atacatatat taaaagggta caaatttgca tatgattttg tgtcaagaag tacattcagt
     3241 attggtggaa taaagcatat aatctcaata ggaatactta aaacaataaa ctgattcaaa
     3301 ctagatgctt tacttgcaat tatcaatcca agcaaagaaa atataataga agaaaaaaag
     3361 gtacctatag ttgatattat aatattatct aaatttaatg taatagcaat aaacatagct
     3421 acaatactag atattactcc taatgaaatt acctttgata aaatatactc agatactttt
     3481 actggagata cagctaagga atttaaaacc ctttgacttt tctcaagaag tacaatagct
     3541 cccataaaaa acaatcccat tgctgctggg tcagaataaa tcataattat agctattttt
     3601 tctctcataa aagttggaaa tacattgata atacaaatat atataatact taaaaaaaga
     3661 tataaaaaat aaaaaccata cttaaattga aactttatat ctcctaaaat aagatttttt
     3721 agcctcattg taaattcctc cctgttatct caataaaaat atcatttaat gttggttcac
     3781 tactatgtat tgataaaagt ttatttttct caataagcat attcaaattt ttatcattag
     3841 cagtattgtt cagaaaacat tcagaagttt tttctccatt atcaaagtaa gtatatctaa
     3901 ctttaatagc tccttttgac ataataagat tatgtggtgt atctaaagca gatattttac
     3961 catttacaat gaacgctact ctgtcacaaa gctctgttgc atctaacatg ttatgagttg
     4021 ttaaaataat agtcttacct ttcgattttt ctgataaaat tatatctttc ataaccttgc
     4081 tatttgatgg gtcaagacca cttgttggtt catctaaaaa taaaatatct ggattatgta
     4141 ataaagcttt tataaaattt aatcgagatt tcataccttt tgaatactca gatacccttt
     4201 tatttgattc attttctagt cctactgatt ttaataactc atcaatagat agtagttttt
     4261 tagaatatag agacccaaaa tattttaaat tttctattgc agtcaacttt tcatataaac
     4321 taggaaattc aaaatcaact cctatatttt cataaaattt atttgagtgt cttttactct
     4381 ctactccatt tacaatgaca cttcctccat aattagttat cattccaatt agaatctttt
     4441 gtaatgtaga ttttcctgca cctgatggtc ctaaaaatcc tagtatttcc ccttttccta
     4501 cttcaaagtt aatattttgt aaaaaatctc tatctgtata actaaatgat aaatcatcaa
     4561 ctttaatcat tcaatatcac cctttcaaat ttttgactat tttgactata tggtcaataa
     4621 aatataattt ttttgtttgt taataatttt taataaaagt tattagcaaa caaaatttaa
     4681 attctattcc ataagttgta taactaatcc gtttatcatt aatcttaaag catcttcaaa
     4741 acaattactt ccaacctcgc gcttatatag catggtaaga aaaactgctc taaatgctcc
     4801 actaaagctt tctatgtctt tattttttgc atttggtatg caagatataa tctctccaat
     4861 ttcaaagtca tcatgtctaa agtattcttc aattatagaa tctggtaact ttctcaccaa
     4921 tatctcaaat tctccattta tcatgagatt taaaattgaa gtactatcaa ccatcctata
     4981 gaatttcata aatatatctg tcaattgttc acatgtaata ctttcatcta ctctatttat
     5041 ctcatataag atttcttttt gtatagagtc atgtatatct cttaatacct caaaaaagag
     5101 taattctttt gattgaaaaa atagataaaa agttcctttt ggtattttta cccgttttac
     5161 aagctcatca acagtagttt ttctaatacc atattgctcc atacacaatc ttgcttcttc
     5221 tttaagtctt tttattatat actctctttc tttatcagaa tatgatttag gcatcttttc
     5281 cctccagttt gactaaaata atttatatag tcaatataat actttttcta tctaatgtca
     5341 atagtattat tagtatattt aaaaataagc ttaaaaactt tcgaaaattc tgaattttat
     5401 atagcatttt ttattatcta gtgttataat taagatataa ataatatatt attctaattt
     5461 tcatatctat ttgcttattt tttaacttag tttctaatag aagtaatcat atttatcttt
     5521 ttaaaagttt caactttaat aaaaaataaa ggagttgatt ttatggctac tactacaaaa
     5581 aaccgttttt acgattatgt tcaagaaaac ttaaataaat atgttgaagt acattcttac
     5641 tttagcagtt gtcctctata tggtgaaatt atagaagcag atagcttatc aattacactt
     5701 tgctcaagat atatagatga atctaatcca actttaaatg aaacttacac attatattta
     5761 ccattaa
//
LOCUS       DBGJFU010000178         5686 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.182, whole genome shotgun sequence.
ACCESSION   DBGJFU010000178 DBGJFU010000000
VERSION     DBGJFU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.182"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            62..691
                     /locus_tag="ACOIAS_003494"
     CDS             62..691
                     /locus_tag="ACOIAS_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703162.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            826..1152
                     /locus_tag="ACOIAS_003495"
     CDS             826..1152
                     /locus_tag="ACOIAS_003495"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGZ2703163.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            1168..2457
                     /locus_tag="ACOIAS_003496"
     CDS             1168..2457
                     /locus_tag="ACOIAS_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGZ2703164.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            2481..2810
                     /locus_tag="ACOIAS_003497"
     CDS             2481..2810
                     /locus_tag="ACOIAS_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGZ2703165.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            2958..4286
                     /locus_tag="ACOIAS_003498"
     CDS             2958..4286
                     /locus_tag="ACOIAS_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HGZ2703166.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            4609..5463
                     /locus_tag="ACOIAS_003499"
     CDS             4609..5463
                     /locus_tag="ACOIAS_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HGZ2703167.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     VEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
BASE COUNT         2276 a          603 c         1045 g         1762 t
ORIGIN      
        1 gcttatatta ttaaatgagt aatataaata attaaaattt aaataaaaag gagtaaataa
       61 tatgcaagat aaaatattaa gagataaaaa ggcaaatgac ccatgggcaa gaatgactac
      121 aagaaacgga tttagtgcag acgaaataat atcgtcattt caaaaagcta taagaagaaa
      181 tatggtggaa gaagcttgtg aatttgctta tgaactatat atatcatcac cagaattaga
      241 agataagcta tggagaagga tacttacaat atcagttgaa gatataggaa tgggggatcc
      301 atcagctgta ataatcataa ataatttaaa tcaagtaaga aaggaatatt catatgcaga
      361 tggagacaga cctttatttt tcatacatgc aataagatat ctttgtgcat gtgagaaaga
      421 tagatcaagt gatttattaa aaaatataat tataaaagga tttgcaatgg gtaaagttcc
      481 agaagttatg gacgtggcac ttgacaagca tacatataga ggattagaaa tgggaaggga
      541 ttcattccac tttttaaatg aagctagtat agtaatacca gaaaaagaag tagataatga
      601 ttataaagaa agatacttaa aaatattaga aaaatataag caagaagaag ttatagatac
      661 ggctttcaag tttaatcatt ggcagtacta agaattagag taaatattaa taagaattaa
      721 aataaaataa taagaattaa agtaaaaact gataggaatt agagtaagaa ttggctgata
      781 aaaaattaga aaataaaaga ggttagatat tgtaggaggg taattatgaa tattttatta
      841 gtatgtgcaa atggagcttc aacaggtgta cttgtggaaa agatgaaaag tttttgtagt
      901 gagcatgaaa agttaaaaac taaaactata aatatagagg caacatcatt tgaaaattta
      961 aaatcataca ttgaagctaa tgatacagat gtagtacttg tagcaccaca aattaggttt
     1021 aaggaagatg atgtagttga agcttgtaag aattacaaaa tagcagttgg tttaatagat
     1081 actaagcatt atggtcgtat ggatgctcct tctgttatga agtcggcaat cgaattatat
     1141 aaaaatagat aattgggggg aataaaaatg gaagcacttc aaaataattt aagaagattt
     1201 cttcttccaa tagcacaaaa gattgaaaaa caaaggcatc ttcaagctat aaaagaaggt
     1261 atgataagca ttaccccaat aataattgtt ggttctttaa gtatgttatt tatggcatta
     1321 aacaatatgt tacctgaagg aagtgcaaaa acattattat ctgaaaatat ggatacatta
     1381 cttatcccta ataaatttac tatgagttta ttatctatat attcagcatt ttttatagct
     1441 caggcactag ctaaaaaata taatcttaat catgtagaaa taggcatgac agcagttgtt
     1501 gcacaacttg ttgtatgtgg acaagtagta gatggtgtat tagatacaag ttatttagat
     1561 gcacaaggtt tatttgtaag tatattagta gcacttttag tagttgatat tactaagttt
     1621 atgaatgata aaaatctcgt aattagattt ccaaaagaag tacctagtgt agtaaataaa
     1681 agttttagaa atttaactcc aatgatagta tgtataatgt tatttacagc aatagcagca
     1741 ataactaaaa atgtctcagg tcaaccacta ccagctatta taatgaattt cttagcacca
     1801 gctataagca gtgtagataa tgtttttgca gtaactataa tactatttat aactcaatta
     1861 ttatggttct ttgggcttca tggagcagca ataacttcaa gtatatggat gccaatagca
     1921 gctacatata tggcagaaaa tgcaacactg atagcagcag gaggagaccc aaaatatgtg
     1981 tttacaatag gattttacta tggtttcttg caagtaactg gttcaggaat aactttggga
     2041 ttagtgtacc ttatgtcaag aagtaagtgt aagagtttta atagtatggg aaaagttgtg
     2101 attttaccat cactatttgg aataaatgag ccggtaatat ttggaacacc aatagttatg
     2161 aacccatata tgtttgtacc atttgtattt ggacctgtat tggttggagc acttaatttt
     2221 atggcattaa aagtaggatt agtaggatta cctattgcag aaccaccagg ctttttacct
     2281 ccaggggttg cagcattttt gatgacatta gattggaagg caatcgtatt agtatttgca
     2341 agtataatac ttatgacctt gatatattat ccattcttta agataatgga gaaggaagaa
     2401 ttaaataaaa atgcagaatt agctacaaca ttagatgatg atagctttga tttttaacaa
     2461 gtcattagaa ggagaaggat atggtacctg aaatggaaga aaaagtattt agtataatat
     2521 cagaagcagg agatgctaaa agtgatgtaa tgatgtctct taaggaaata aaaaaaggtg
     2581 actacaataa agcaaaacac cttttaaatt cagcctcaga gaaaatacaa acagcatcaa
     2641 agcatcattt agaattacta tcaaatgcta tgaatagtga agatagtggg actgattttt
     2701 tagttgtgca ctctgaagac cattattcaa atgcattatt tgctcattct ttagtatctg
     2761 aacttgtaga tatatttgag atgatggacc caagaattaa taagaattaa atttcttgta
     2821 aatagtgctt gaagtttggc tatagtttat taaatcaata gatttaataa atgaactccc
     2881 ttttacttta aagtactagt atttataaat ttatttataa atacagtaaa tttttaaata
     2941 gaaaaggaga tattattatg gaaaaatttt cagtagtaat agctggagga ggaagtacat
     3001 ataccccaga aatcatatta atgttgctag ataacttaga taggttacca ttaagagcaa
     3061 tcaaattata tgataatgat gaagaaagac aaaataaggt agctaaagct tgtgaaatac
     3121 taataaagga aaaggaccct aatatagagt atttagcaac aacatgccct aaagaggctt
     3181 atacagatgt agatttttgt ttagctcata taagagttgg aaaacttgaa atgagagaac
     3241 tagatgaaaa gatacctcta aaacatgggg tagttggtca agaaacttgt ggaccaggtg
     3301 gaatagctta tggaatgagg tctatagaag gtgttcttga aaacatagat tatatggaaa
     3361 agtattctcc aaattgttgg atgttaaatt attctaatcc agcatcaata atagcggaag
     3421 ctgttagaag gttgagacct aattctaggg taataaatat ttgtgatatg ccaataggta
     3481 tggaacataa tatagcaagg attgcaggac tcaaatctcg taaacatatg gatataagat
     3541 actttggatt aaatcacttt ggttggtata cttcaattaa agataaaaaa ggaaatgaac
     3601 ttttaccaaa attagtagaa catataaaag aatatggttt tataaatggt gaagaaggaa
     3661 tgaaaaatga caagaaagat agttggtttg aaactaattt atttactaaa gaaatagtaa
     3721 aaacagattt aactacaata ccaagtagtt acttaaagta ctatctattt ccagattatg
     3781 ttgtaaatca ttctgatgta aattatactc gtgccaatga agttattgat ggaagagaaa
     3841 aagaagtttt tggttcatgt gcattgatag aaaagcaagg acattctaag ggcagtgggc
     3901 ttaagatagg aatacatgca gagtttatag tggatttagc tacagctcta gcttttaata
     3961 cacaggagcg tatgttattg atagttcaaa ataatggagc tatagaaaat atacaagatg
     4021 atgctatggt tgaaattcca tgtatagttg gaaaagacgg gtatgaacca ttatcaatag
     4081 ggaaaatacc aacattccaa aaaggtttaa tagaacaaca attagcagtt gaaaaattag
     4141 tagttgatgc ttggattgag aagtcttata ataaattgtg gcaagcactt atgctatcta
     4201 agacagttcc tagtgcaaca gttgcaaaag aattactaga tgatttaata gaagcaaata
     4261 aaaattattg gccaaaactt aattaagaat aatatatttt taaagttcct acagtttagt
     4321 acatagccta gttaatgtat ttgtagggac tttatttatc tacaaatgag atacaagtgg
     4381 caaacatagt gtattttgca gttaggaaag taggtttagt tacttgttag ttggattaaa
     4441 tttaggtaca ataatttact atcaatcaat agaaataacc agagaagttg tataataaaa
     4501 aatttacact tttaacagga tttatgtagg tgtttataga aataaataaa tatgtacata
     4561 aatacataat tatttttaag tctaaaatac aagaatggag agatggctat gaagtggaga
     4621 gattatgctg atgatgtaaa tttatttgag gattttgata aaactatcaa gcctctaact
     4681 gacgaacaaa gaagaaaaaa tataaacact ttaattgcat atttttcaaa agaagttcct
     4741 tctaaaatat acaatttatc aaatgatgaa attaagccta gagatatact tagaggactt
     4801 ttgaatgtat atccaccaaa agaaattgct ccagagatac ttaatatgtt acataattta
     4861 ttgttgattg aatgtgaaga aagagagtta gtagatgtaa atgatataga agaagtagag
     4921 gaagggattg ccatatggag aggtaatata acgaatttaa gagcagatgc aatagtaaat
     4981 gctgcaaata acaagttatt agggtgttta caaccattac atctatgcgt ggataatgag
     5041 atacattcat gcgcaggtcc tagacttaga gaagattgtg acaaaataat aaaaaaacaa
     5101 gggcatttag aatatactgg agatgctaag ataactagag gttattgttt acctgcaaaa
     5161 tttgtagtac atactgttgg acctattgta tctggtggtc agccaagtaa agagcaagaa
     5221 aagcaactgc ttcattgtta taaatcatgt ttaaatacaa taaaagaaat tgatgaaata
     5281 aaaaatattg ttttttgtgg aatttctaca ggtgtatttg gttatccaaa aaaagaggca
     5341 gctaatcttg ctgtaagtag agttagacta tggctaaaag aaaatcctga aaaaaattta
     5401 aaagttgtat ttaatgtgtt tactgaagaa gaagaagaga aatatagaag aatatttaaa
     5461 taatattatg taacagaatc ctaaaaatac ttattatata atacaaattc attgtataag
     5521 ataataattt acaagtagta aaaattaata aaatataatt tgtattttta ataaataagt
     5581 aggtataata gaaattataa gaatagtata atcaattatg ctattactta gaaaatataa
     5641 tataggaaaa ttgtatggta aataaatatg aaaaatagtt ttaaaa
//
LOCUS       DBGJFU010000179         5667 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.183, whole genome shotgun sequence.
ACCESSION   DBGJFU010000179 DBGJFU010000000
VERSION     DBGJFU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.183"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            132..632
                     /locus_tag="ACOIAS_003500"
     CDS             132..632
                     /locus_tag="ACOIAS_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HGZ2703168.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            689..1675
                     /locus_tag="ACOIAS_003501"
     CDS             689..1675
                     /locus_tag="ACOIAS_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2703169.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1725..2447
                     /locus_tag="ACOIAS_003502"
     CDS             1725..2447
                     /locus_tag="ACOIAS_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HGZ2703170.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2476..4410
                     /locus_tag="ACOIAS_003503"
     CDS             2476..4410
                     /locus_tag="ACOIAS_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2703171.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKQEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4403..>5667
                     /locus_tag="ACOIAS_003504"
     CDS             4403..>5667
                     /locus_tag="ACOIAS_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGZ2703172.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLILLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYK"
BASE COUNT         2381 a          428 c          969 g         1889 t
ORIGIN      
        1 aatgaattgg aaaaatagtt cgataaaata atttgttttt ataaaattac tgtttaagtt
       61 tatagatttt gtataaaata aagttatgtt attaattttt ttagaaataa aacttttact
      121 aggagacaga tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa
      181 tctatgcagt tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg
      241 tagcagagat tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat
      301 taggttgttt tttagcaaat gtaataggac catatggtgt acctgatata atttttggaa
      361 cattagccac atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaga
      421 ataaaagtac tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg
      481 gtctaatgtt aaatgtattc tttgggcttc cattaattct atctatgata caagtaggtt
      541 ttggagaatt tgtagtagtg acaattgtag gagtaccatt tttctgcttt ttaatgaaaa
      601 aatataaaaa tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag
      661 agtattatta gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg
      721 gatatatatt aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct
      781 ttcagctaaa acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca
      841 agcagaacaa ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt
      901 tatatttaca gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta
      961 taattataag ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg
     1021 taaaagcagt ataaaagtag gtatagaaaa tattaaaaga tacattggct ttgtagctaa
     1081 tgatactaat gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc
     1141 agaatctcta attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt
     1201 atgccctata ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgtaa
     1261 aagtgataaa ataatattag ttcatataga taatataatt gatataaaaa gaacaggaga
     1321 aagtaaaaaa atacatataa acttggaaga atggttacat gaagtatata acaatttata
     1381 tggaaatagt tcaactacta aaaaatttgg tgaaattatt tatctaaatg tattattaac
     1441 taaagaaggt gtttctaaaa taaaggacag ttcttggaat ttagatatgg agctaaatga
     1501 agatggtagt ggaataatta gtacattcat aaaggttgaa gatatagatt gttatgcttc
     1561 gattttattt tttattggaa ataatgctaa ggttatagag ccaaaatttt tgaataagct
     1621 actttatgat aaggccaatg agttaagata tttttatgaa gaaaatatgg tataatattt
     1681 agaaatgttg aaattaaata attattaagg agatatacgt tataatggaa aattttaata
     1741 agaatgctaa aataattggt attggagctg aaggtattaa tataataaat gaagtagaag
     1801 aaaaaataaa agcaaatatg gatatagaaa aaataaacat aaatcaagag attgaaaaag
     1861 aatatgttag aagtctttta gatggtgtag atatattatt tttgatatat tcatctgaag
     1921 ataaacatat tagagatatt ataaaagctg tttcatatat gtcaaacgaa agaagagttc
     1981 tatctatagg aatggattgt tcagagaaag aaaataaaga agatttagag cttgggagag
     2041 agtttaaaat caataatgat agtatattta aatttgttga tttaatgaat atcatggtag
     2101 agtctatttc tgattcttgc atgataaata tagatattac tgatttaaaa gaagctattg
     2161 taggagataa aggaattaag tattcttttg aagagtttga agatacaaag tcatatagcg
     2221 agatagctga cattttattt gacaggatgg aatatattgg tgaggaattt ataagtaaga
     2281 aaggaattgt atttgttgaa ggaagtccag aattttctat aatggaattg aatgatttaa
     2341 taagtaatat tcagagcaag gtagaagaaa gctatgaagt aatattttct ttatatataa
     2401 aggaaaattt aaatggaaat attagagtag gcttattgta taattagtat attttagata
     2461 ttaaggagag tgtatatgtc tgaaaatata agaggtacag agtattggtt aatggaagaa
     2521 tttttttatc aggaaacaga cacaccacag atttttagag aagaaagcat aattgcactt
     2581 ggatttgaca taaaaataaa tttaaaaaaa gtattcagta tggggaaaaa atcagaaaag
     2641 tatattaatg agaattgtaa tgataaagat tttataaaaa agtttatgtc gattaaaaaa
     2701 ggagattatt ttttatataa agcatatcaa aatatagaag aaaaagttaa atgtaatgtg
     2761 tctattggca tagttgaaga agattttgaa aatggatatg aattaagtca tattttaggg
     2821 catacactac cagtaaaatg gatgcataat tttgatacag tttcaaattg taaagtatat
     2881 acaagtgaat tttataaatt aaataataat gatattagct catatttaga aattattaag
     2941 tcacaagata agaaattatt gagaaaaaga atagaagaaa gtgaaaataa taaatggtat
     3001 ttaaaaaact attattataa aaatgtagat aagtgtgtag atgatgaaga attatttatt
     3061 cagcccatta atctagaaca attagaaagc ctaaaaaata taaagacaga tgattatatt
     3121 ttattgtata atagcgagaa agtaatttgt gaaaatgacc atacaatgaa gagtctattt
     3181 ggcgtatgtc aagttatgga agggttcgat aaagagaaag taagtgcttt ggaaggtgga
     3241 tatttttttc ctgttagatg gatagagtat gaaaaggaga atatagacgg aaatgcatgg
     3301 gttatatttg aaaataatag tatagttgaa aagtatgtaa aaagatttac taataaaatg
     3361 ccattaaata ctatacttta tggaccacca ggaactggta aaacatacaa tacaaaacag
     3421 atggtttaca atctagtaag tgaaaccagt ctaagaaaca aatactatac aggttactta
     3481 gattataaaa acaatgtaga attttgtaca tttcatcaat catatggata tgaggaattt
     3541 atagagggtc ttaagtctga tggtgaaggc aattttaagc aagaagatgg gatattaaag
     3601 gatatatcta tagaagcatg ttataatgca ttgaaatttg aaaaaaagat gagctttgag
     3661 ttggaaaaag atagcttgac tttagaaact ataaaagaca ggaaaaagaa attagtatta
     3721 gaaaatatag aatatagtga gagttttaat tttgaagaat cagaaaacta tgttttaata
     3781 atagatgaaa ttaatagagg gaatgtatct aaaatatttg gtgagctaat aacattattg
     3841 gaagatgata aaagacttac taaatcaaat caaactattg tcaaactacc atattcaaag
     3901 gaaaaatttt ctctacctcc aaatttgtat atagttggaa ctatgaacac atcagataaa
     3961 tcaatagcac ttttagatat agctttaaga agaaggttta attttgaaga aattatgcca
     4021 gattatgatt tattgtctat tgtagatggt atagatatta gaaaaatgct atatacaatc
     4081 aataaaagga tagagttttt atatgataga gatcatgtta ttggacaagc ttatctactt
     4141 gctgtagaaa atattaatga tatagtagta gtatttagaa gtaaaataat accacttctt
     4201 caagagtatt tttattatga ttcagaaaaa gtaggtttga ttttaggagg aattggaaga
     4261 aatgaaaaag ataaatacat tgtttataaa gaaaaactta tagcttctga gttatttaaa
     4321 aataataatg cctcaataat ggagagtaaa gttaactatt gtataaaaca aaacatatct
     4381 agtgaggagc taaaaaatat atatgagtaa aaatatatat gttaaggaaa cctatgaatg
     4441 gataagggta ggtaatggtg aaaatgagct tacagaaatt gaatatgaaa aattgcttaa
     4501 atatctagag aacaataatg atgtattaaa aagtaatata atagatatta aatacaaaaa
     4561 acttagattt ataaactatg taggcatcat atgttttgaa aatgtaatac ttgaaatact
     4621 tcctaaattg tcattaagtg ataatcttgt aaaagataga gagatactat tacagatgct
     4681 ttctatatgt aataaaattc caattacaat gaatgaaaaa ataagattga gtttgaaaaa
     4741 ttataatctc ttaaattttt ttgttatgta tttcattgag agtatgcaaa cacagatgaa
     4801 aaaaggtatt tattttgaat atataaataa aatagagaac ttaaatgtta tgaggggtaa
     4861 aatattatta agtacatatg caaaagaaaa aggcatttct ccaatgaaaa taagatgtga
     4921 atatgatgag tatagtgaaa ataatttctt gaatcaagtt cttaaaaaag catgtatatc
     4981 tattttatgt agaataaatg acaattcaat tcaaggcaag attaaaaaaa ttttaagcta
     5041 ttttcaaaat gtagatttaa tttatataga tagaaaaaaa ctattggatt ataaatttta
     5101 taaaaacaat gatagattta aagattgtta tttactagct agacttatac ttttaaattt
     5161 gtctatggat aatagtcaaa ataatcaaga ggcattttct atattatttg aaataaatac
     5221 tttgtatgaa gaatatatag gaattttaat caaaagtata tgggacaatt catttagaga
     5281 aacatatata caagataaga gtaaatttct tttgaaaaat gaacaaacag gtaagaaaaa
     5341 ctttaattta agaccggata tagttttata tgacttaaaa aatgaatatg aaattataat
     5401 tgacactaaa tggaaagcta ttgaagtaga ttcaaatgtt ttttataggt caagtgatat
     5461 ataccagatg tatgcatata taacagcata tgaaaatgca aaaagatgta tattattata
     5521 tccttgtatt caaaaagata agaattatag ttcctggaaa ttatcagaat catttaaggg
     5581 taagtttata gaagcaaaaa ctgtgaggtt agatgatata aaaaacacaa aaaatgattt
     5641 aaaaaagata atttttaatt ataaatt
//
LOCUS       DBGJFU010000180         5583 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.184, whole genome shotgun sequence.
ACCESSION   DBGJFU010000180 DBGJFU010000000
VERSION     DBGJFU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.184"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     regulatory      37..139
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            233..1609
                     /locus_tag="ACOIAS_003505"
     CDS             233..1609
                     /locus_tag="ACOIAS_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGZ2703173.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1723..2868)
                     /locus_tag="ACOIAS_003506"
     CDS             complement(1723..2868)
                     /locus_tag="ACOIAS_003506"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGZ2703174.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3121..3828
                     /locus_tag="ACOIAS_003507"
     CDS             3121..3828
                     /locus_tag="ACOIAS_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703175.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3875..5470
                     /locus_tag="ACOIAS_003508"
     CDS             3875..5470
                     /locus_tag="ACOIAS_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703176.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMLIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
BASE COUNT         1887 a          651 c          880 g         2165 t
ORIGIN      
        1 taattatata aattaaaata atattcgtaa agaaaaattc atgttttgta ctgtataaca
       61 tataattttg acaatatggg tcataagttt ctaccggaat accgtaaata ttctgactat
      121 gtatactatt atttagttaa ttaaatattt aattaactga attatgaagg atacggctca
      181 gcttatcaac tactgtgtcg tttttttgta aaatttaata ggaggtgttg ttatgcaaac
      241 aacaaacagt agtactatgc agaaactttt tccaattttg actaataaaa atgtcgacat
      301 gaagaaggaa ataattgcag gagtaactac ttttttaact atggcttata taatagctgt
      361 aaatccaaat attttatctg agacaggcat gcctgcaggt gcacttgtta caggtacttg
      421 tttagcagca gcatttggat gtattttaat gggtgtagtt gctaaccttc cttttgcttt
      481 agcatcaggt atgggtctta atgcattttt tgcatataca gtagttttac aaatgggtgt
      541 tccttgggaa gtagctctta cagcagtatt tgtagaaggt attattttta tagtattatc
      601 agtaagtgga gttcgtgagg ctgttgtaaa tgcgatacca aaaaatatga aattagctgt
      661 tacaggtggt ataggaatat ttattgcact tataggtcta gttaacagtg gaattgttat
      721 tggagaccaa gccactctta tcaaaatggg tagatttaca ccagcagtaa taataacttg
      781 tgtaggatta ataattattg ctgtattaga taaaaaaaga gttaaaggtt ctatactttt
      841 tggaatagta gtgagttcat tacttgcttg ggcatttgct tttataaatc cagagtatgc
      901 acaaaaatta ggtatatatt tacctggtgg attatttaaa ttcgaaagtt tagcaccaat
      961 agcaggtaaa atagatttag gatatgtact acatccaaca aatataggtg gttttttagt
     1021 agtagtttgt acatttttat ttgttgattt ttttgataca gtaggtacat tagttggtgt
     1081 ttgttctaaa gcaaacatgt tagatgaaaa agggaatgtt cctaatgtag gtagagcctt
     1141 acttacagat gcggttgcaa ctacagttgg tgcaggactt ggagtatcta cagttacaac
     1201 ttatgttgag agttcaacag gagttatcgc aggtggaaga actggatgga cagcaataac
     1261 agttggaatt ttattcttag ctgcaatgtt tttctctcct gtatttatag caattccatc
     1321 atgtgctaca gcaccagctt taatatatgt tggatattta atgcttggta cagttaagga
     1381 tatagagttt gataatataa ctgaaggtgt tccagctttt gttactattg cttgtatggc
     1441 attaacatat agtataggtg atggtttaac tttaggtata ttaacttatg ttttcgtaaa
     1501 tatattttat aatatatttg gagctaaaaa ggcagaagac aaaaaacatg tttctgtagt
     1561 aatgattgtt ctggcaatat tatttataat aaaactttgg ctactataat aggtattatt
     1621 taaaatttta atttaatata aaataaaggg ctgtaattta ttatcaattg tgatgataaa
     1681 ctacagctct tttgtatttg ttgtattttt gtatttattg tattatttaa taatgtctat
     1741 tagttctcca ttttgtaaag ttttaggtgt aattactata ttgaactctt tttgcaaatc
     1801 ttttataata tctaattctt tttttgttat tattgagatt gtgttaccac ttcgatttcc
     1861 tctagcagtt cgaccacatc tatgcaggta ttcattttta ctttttggaa aatccaagtt
     1921 aaaaacatga cttacatcca caatatctag tccacgagca gataaatcag ttgtaattaa
     1981 aatctttgct ttaccaagtt taaatttatt tattgcattc tttctatctt ctttttccat
     2041 atttccaaaa attcctattg ctttgtaatt atgataattt agttttgata ctaggacttc
     2101 tatatttttt tcattattta caaatacaat agcttttttt ggatttgtag ctgctaatgc
     2161 ctttctaaga aaagtaaatt tatcccttat ctcacctaat aaatatgagt gattgatgtt
     2221 aggatttatt tgtgattttt cctttgtttt tattatttca aactccttca taagtttatc
     2281 acaaattttt atagtgctat cagtaagact tgcagagcat cctataattt gtctatctct
     2341 taacgttgtt cttattatgt cttctataca agttatattt tttccattca atagattgtc
     2401 cacttcatct aagacaatag tttttatatt atgtgatttt agtttctttt gttttatcaa
     2461 gtctaaaact cttccacaag agccaattac tatgtgtggt tttactgctt ttatattttt
     2521 tatttgtttt tgtatattga cctccccaat taaagctaaa gaagtaacgg agagttctgc
     2581 atttttagcc aataattcaa cctggtttgt aatctgcatt actaattcat gcgtaggtgc
     2641 aagtataaga gcttgtgttt ctctttttga tgtatcaatt ttttcaaata taggtaaaag
     2701 gtacgctagt gtttttcctg ttccagtctg tgaattaatc aataaatcct tattttgaat
     2761 tatattccct ataacaagtg actgaacttc tgttggagaa gttatgtctt gttttttgag
     2821 tccatctatt agagtggaac ttatttttaa ttgttcaaat gtattcatat tttactcctt
     2881 atattttaat aattatagtc aactattttt tattatacat tgtttattca aatcgaacta
     2941 tatattcttt tataattatg tgtaatttga aagtaaatat caattatatt tataccttta
     3001 tatttctgat acctttgcat tttatagatt tctaaaataa acaaataaaa gttgtaattg
     3061 tttgatattt gttatatatt gtatataaca aatatcaaaa ctataatatg gagggaaatt
     3121 atgttaaaag taatcaatgt atctaaatca tataaaaata aaaaagtagt aagtagtgtc
     3181 tcatttgatg taaatgatgg agagcttttt gggtttatag gacataatgg agcaggtaaa
     3241 actactacta ttaaagcaat tgttggaata cacgattttg aagagggaga agtactaata
     3301 aattctaaat ctataaaaaa acagcctgtt gaatgtaaaa aggaaatggc atatatacct
     3361 gataatccag atttatacga gtcattaact ggtatacaat accttaattt tatagctgac
     3421 atatttgaag ttgccaaaga taaaagagaa gagttaataa aatattattc aaataagttt
     3481 gaactcaata aagcattagg agatttaata tcttcatatt cgcatggtat gaaacaaaaa
     3541 ttagctataa tttcggcttt gatacacagt ccaaaaatct taattcttga tgaacctttt
     3601 gtaggtcttg acccaaaagc ttcctttata ttgaaggaaa ttatgaggga gttttgtgac
     3661 aaaggtggtt gtatattttt ctcaacacac gttttagaag ttgctgaaaa aatttgtgat
     3721 aagattgcca taataaaaga tggaaatata attgcttatg gaactacaga agaagttaag
     3781 ggaagtaatt cacttgaaag tatctttatg gaattaatag agaaataaaa tgttttttat
     3841 acaatatagt gtctaaagtg aggaatgata aaatatgagc aatctaagta tattaataaa
     3901 aactaatata ataaatgaat ttaaattaaa tgcgttaaaa aaagctgatg gtagagaaaa
     3961 acaaaaaata atgggaatga tgctttcagt tgttataata gcgggtctat taatttttta
     4021 tgtaacaagc ttaagtctct ctttagttga tatcttaaag caaataaatc aactggaaat
     4081 gctactgata tttggttttg gattatcaac attaacaact attgttacat cattatataa
     4141 aacgtcatca tatctatttc aagcaaaaga tttagacctt ttgactagtt tacctataaa
     4201 agaatctact gtactagcaa gtaagatatt aatgttggtt ggagcaaact atttattttc
     4261 ttcaatatgt atgcttatac ctgcagtggt ttacttttta aattctgatg taagtataac
     4321 ttatttccct tatttgatat tgctattttt agctttacca ttattaccga tagttgcttc
     4381 atcaataatt tttctattta tagggcagat atcatcaaga ataaaataca aaaatttagt
     4441 attaatcata ggtagcatta tattgatgtt gctttatacc ctacttatgt caagacttaa
     4501 ttctatgtca gttgaaatgc taaagaatag tgtgtctatt tcagagacga ttagaaaagt
     4561 atatcctcct atatattatt ttgttgatgc tcttaaaaca ggaagtatag tttcattttt
     4621 agcatttata gccatctcaa taataccatt ttttatgttt gtaactctgt ttgcaaaaaa
     4681 atttaaagat ataaatgcta agatgagtga aggatataag tcaaaaaatt atgaatttaa
     4741 gggtcaaaaa acttcaagac cttcaaaagc tttattaaaa aaagaattta agagatatac
     4801 ttcttcgttt atatactttt taaattcatc tgcaggtttg ttatttctaa tagtaggtac
     4861 aattttaata ttattatttg gagcagataa agtgggtgag ttactgaaat tgaatataga
     4921 ttttaatctt ataaaaatac aattattggg agtattcttc tttataatag taatgaattg
     4981 tacaacatac tgttcaattt ctcttgaagg aaaaaactta tggatactga aatcttctcc
     5041 aatagatgaa agagaaatat tctggtcaaa aatattgatg aattttttat tgaatgcacc
     5101 actatctatt ttatgtctaa tattagtatc tataagatta gactttgaaa ttcagtttat
     5161 tattgtatca atctgtttga ttgtaagttt ttcaatgttt gtagcattga tgggattatt
     5221 atcaaattta ttatacccaa accttgattg gaaaaatgag gtggctgtag taaaaagaag
     5281 tgcaagtatg atagttacaa tgttagtatc attagcttat gtaattatac ttggaggtat
     5341 atatgtatta cttggaattt cgtttttaaa tatatatatt attttggcaa gtatacttac
     5401 tttgatatta gattttatga tatggagaat tatttctact aaaggagtaa gaatatttaa
     5461 aaatttatag gtgtatttat gtcaaataaa gacttatctt ttttagtctt gcaaataata
     5521 ggttaagtag tatactatgt atagtgatat gaaaaaattt aggagaaaat aaatgagaaa
     5581 att
//
LOCUS       DBGJFU010000181         5498 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.185, whole genome shotgun sequence.
ACCESSION   DBGJFU010000181 DBGJFU010000000
VERSION     DBGJFU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.185"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(678..2813)
                     /gene="rnr"
                     /locus_tag="ACOIAS_003509"
     CDS             complement(678..2813)
                     /gene="rnr"
                     /locus_tag="ACOIAS_003509"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGZ2703177.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3406..4020
                     /locus_tag="ACOIAS_003510"
     CDS             3406..4020
                     /locus_tag="ACOIAS_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGZ2703178.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            4038..4400
                     /locus_tag="ACOIAS_003511"
     CDS             4038..4400
                     /locus_tag="ACOIAS_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2703179.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4506..5003
                     /locus_tag="ACOIAS_003512"
     CDS             4506..5003
                     /locus_tag="ACOIAS_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HGZ2703180.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1806 a          839 c          682 g         2171 t
ORIGIN      
        1 tttaatatct attcttaatt aggtattttt aaattatctg tttacattaa atatttctta
       61 attacttatc tttcagctag ctttttctat taaatgtttc tcaattactt atttttcaat
      121 tagccacttc tattaagcat ttcttaatta cttatttttc aattagtcac ttctattaaa
      181 catttcttaa ttatttattt ttcaattagc cacttctatt aagcatttct taattactta
      241 tttttcaatt agccacttct attaagcatt tctcaattac ttatttttca attagccact
      301 tctattaagc atttcttaat tacttatttt tcaattagcc acttctatta agcatttctt
      361 aattacttat ttttcaatta gccacttcta ttaaacattt cttaattatt tatttttcag
      421 ttggcttttt ctattaaatg tttcttaatt acttatcttt cagttagctc tttctattaa
      481 acatttctca attacttatt tttcaattag ctactttcat taaatatttc ttaattattt
      541 atctttcagt taactatttc cattagatat ttctcaatta cctattttta tcatttattt
      601 tatagagtat tgtgcactat atatttatac atatcataaa tactacaact tgttatctct
      661 tattttagct gttttatcta ctcctctatt ttttcaacta tctcaaagtc aatttcttta
      721 aaatctacat taacactttc aacctttatc ttaacaaggt cacctatctt atatgacttc
      781 ttagttcttt ctcctaaaac actataagaa ctctcatcat aaatatagta atcatcattc
      841 atatttgcaa gtcttatcaa cccttctatg gtattatcta actcaacaaa tattccaaat
      901 gaagttacgc tagatacaac accctcaaat tcttgaccaa ctttatcctt catataaaga
      961 gccttataga aatcatgtac atctctttca gctaactctg cctttctttc tttctcggat
     1021 gattgaacag atgcataatc cactatagta gtaagctgtt cttgtctttt tccacttatt
     1081 ttattattta aagattcttt tattattcta tgaatttgaa ggtcaggata tcttcttatt
     1141 ggtgaagtaa agtgacagta gaatttagct gctaatccaa aatgtccact acattctggt
     1201 gagtacttag cttgttttaa agaacgaagc attatagtac tgatagctcc ttcttctttt
     1261 ttgcccttta ttttctctat tataccttgt aatgcttttg gatgtacttc ctctttatca
     1321 ccttttatta tatatccaaa tgtagatata aacttactca attcttccat tttttccaaa
     1381 gatggtgtct catgtattct atacacaaac ggtattccca tccagtaaaa atgttcggca
     1441 attgtctcat tacttataag cataaattct tctattatct tattagatat tcttctttca
     1501 tatggcttta tatctacaac ttcgcctttt ccatttagaa ttatctttgc ctctgggaaa
     1561 ttgaagtcta tagctcctct tttttccctt cttgacataa gaatcctagc taaaatttca
     1621 gcatttttaa aatcttcaac aacatgttca aaagtctttt taagtttttc atcatccttt
     1681 tctaatatat cagatacttc tgtataagtc attctagctt ttgaatttat tacagattca
     1741 catatttcat gtttaaccac atttccttta tggtctattt ccataaatat agaaagagtt
     1801 agcttatctt caaatggatt taagctacat actccatttg aaagagtttt tggcaacatt
     1861 ggaattactt tatctactaa ataaactgat gtagctcttt ttaaggcttc cttgtcaagt
     1921 ttatttttct ctcttacata atgtgtaaca tcagctatgt gaactcctag tttgaagttt
     1981 ccattaggta atacttctat tgatatggca tcatctaagt cttttgcgtc atctccatct
     2041 atagtaaata tgtttaaatc tcttaaatct cttcttcttt ttatctcttc ttgaggaatc
     2101 tcaacagcta cagcttctgc ttcttgcaat actttctttg gaaattcttc tggaagacca
     2161 tgttctctta ttatagaatc tatttctacg cctctttcac ctttttgacc tagtatctct
     2221 ataattttgc cttctggttt tctatcctct tgtggccata ctgtaatttc acatactacc
     2281 ttatcattat ctactgctcc actaaaatgt tttttaggta tatatatatc tttagtaaat
     2341 ttcttgttgt ctggtgttac aaatccaaaa gttttacttg attggaaggt acctactatt
     2401 ctagttattt ctctctttat tacttttact attcttcctt ctgctctctt ttcttcagtt
     2461 gcaggtacta ctatttctgc cataactcta tcattatgca tagcaccatt tatattgtca
     2521 cttgagataa ataaatcctg tgtatattca acatcagatt ctacaaatcc aaaacccttt
     2581 ttgtgtgata caaattttcc aacaaagtat cccatttgat tgggagatac tatttttcct
     2641 tttttagtaa agcagatata accatcttct tctaattcat ttaaaaaatt ataaaacatt
     2701 ggcatttctg atgaatgtat atcaaatatt tctgctaatt cttctttttt tagaggatta
     2761 tatgcacttt catttattaa tcctagcaaa ctttctttaa gtcctggtat catttttccc
     2821 tcctgtttta cttttcatta tattattatt tatattgtct tttatatttc ttcagacaaa
     2881 acatttttta cttatcacat aattttctat attttcttat tattataagt taaaattact
     2941 tatttatatt tttgatttaa tgtaatcttt gtcatactaa cttcaaacaa attatgatgt
     3001 tttataaatt atttgtaaat atcattataa ttacttaagt tattctttat cataattgct
     3061 taaattactc tttatcacct aattatacac catcaaatag gattggtaaa atattcatct
     3121 catataagta ttactatata ataatttatt ctattagtat actcaaatca tgtaaaattt
     3181 atataactca attaattttt taaatacaac tttataatat aaaacttata ataaaagtaa
     3241 atttatatgt taaatgaggt tgatttttta ttatttttta tttatgctag tactaaagac
     3301 ttgcaaacgt tttttgacaa gtctttattt tgtctaaatt aaacaaactt ttttacaaat
     3361 aaatacataa aatttaaact aatctattta ttggaggaga taaaattgga agaagtaatt
     3421 gcaaaaatta ttactataga aaatggattc aaagaaattg aaaacattgc taaaaaaatt
     3481 gtaaaaaata atgactcaaa aaagtgttat tatctatcaa tctacttata taggtcagaa
     3541 tattatcaag taagagaatt ggctgtgttt atacttggtt ttatatcagt aactatttct
     3601 gaagctttgt tatttttaaa agataatgtg agtaaagatg aaaactggag agtacaagaa
     3661 atactagcta aatcatttga ttattattgc agtgaagtag gatatgaaaa ggcacttcct
     3721 gttataaaag aatggcttaa ttctgatagt ccaaacataa aaagggctgt gacagaaggt
     3781 cttcgaattt ggactaatag agactatttt aaatctaatc ctgacgttgc tatatcacta
     3841 ctttctagtt taaaagaaga tgatagcgaa tacttgagaa agtctgtagg taatgctctt
     3901 agagatataa gtaaaaaata tccagattta gtaaaaaaag aattagattc atgggatata
     3961 tcaaataaaa aagttgagtt tgtatataag cttgcttcaa aatgtatctt ggcaaagtaa
     4021 aatggaggtg aacttatttg aaacttcgtg atgagtatac ttgcccatta gaactaacac
     4081 atgatatgat aaaaggcaaa tggaaatcta ttatattatg gagattacgc ataggaccaa
     4141 cttccttatc aaaacttgaa aaggaaatca atggtattaa tcaaaagatg ttacttgaac
     4201 aacttaaaga attaattgaa tttggtcttg tagataaaaa gacatatgaa ggttacccac
     4261 ttaaagtcga atactcactt acacctattc gagggaaaca aatattggat gctttggcta
     4321 taatgcaaga tataggaatt agctttatgt tggataatgg tatggaagat attcttaagg
     4381 aaaaaggtgt tttaaaataa atagataccc acaaaaaagt aagtaattta ccattatttt
     4441 caactggtat ataattaaat tatagatata ttctattaat ttattataat aggaggtaaa
     4501 ttattatgaa agtaactagt acaggtatag taaatggtgt tatagaagat aaatatggta
     4561 aaagaggaac tcagttcaat gaaggtggta tgcctacata ttcactacct ctaaagtttg
     4621 aagatgctcc aaagaataca gtatcctttg ctgtattttt agaagataaa gactctgttc
     4681 ctgtatgtgg gtttgcatgg atacattggt tagctgtaaa tataacaaaa gatgagttaa
     4741 atgaaaatga aagtgtaaca gctactgatt ttgtacaagg gactactagt tggcatggta
     4801 aactaggagg tcttgacaga ttagaggcca gtgtatatgg tggaatggct cctccagaca
     4861 gacctcatgt ctatgaaata catgtatttg cacttgatac tatccttgac cttgaaaaag
     4921 gattctatat gaatgaattt ttcaaagcta ttgaagggca tgttttagat acatttacac
     4981 taaaaggaag ttattctaat taattaggtt gactatagct aatcattaat taaatgatac
     5041 atttaattga tgattagctt aattgttatt agaatatttt ctgcacttta tcttaatata
     5101 tattttagtt gactggtatt ttaatttcta ttagattatt atcaatttat ataaattgat
     5161 taaaaaatag ctacgcacac tcgtgacttt agtcatgagt tagtagctaa aatagtcagc
     5221 atatagggaa acttatatgt agagatagga tagaacctat ccaatactac ttgaattgct
     5281 aaaaacccct aaagctaacc aaactaaaac ataaggatga aataaaccta agtgtgaaag
     5341 ttacgaaagt agaaaaaatt ggttagatgg tataaggtta aaccctaagt actttaaaat
     5401 gggcaatcag caggtaaact ccaaatagga gaaacttcaa cgactattcc tcttgaggga
     5461 agtacactac aagttatcgg tagtggaagt gggtagac
//
LOCUS       DBGJFU010000182         5477 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.186, whole genome shotgun sequence.
ACCESSION   DBGJFU010000182 DBGJFU010000000
VERSION     DBGJFU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.186"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            17..1420
                     /locus_tag="ACOIAS_003513"
     CDS             17..1420
                     /locus_tag="ACOIAS_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2703181.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1639..4116
                     /locus_tag="ACOIAS_003514"
     CDS             1639..4116
                     /locus_tag="ACOIAS_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2703182.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4131..4817
                     /locus_tag="ACOIAS_003515"
     CDS             4131..4817
                     /locus_tag="ACOIAS_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703183.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5091..>5477
                     /locus_tag="ACOIAS_003516"
     CDS             5091..>5477
                     /locus_tag="ACOIAS_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HGZ2703184.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINT"
BASE COUNT         2091 a          593 c          866 g         1927 t
ORIGIN      
        1 gaaaagagtt gattaaatgc ataaacctag ttataaaaca gtatttcatt catacattat
       61 ttttttcatg atttttattt gcttaatcct gacagtaata ggtattttct tatcacttat
      121 tactattcag aaaccaaatg gggaatttat tcgtagtgac tggccgaaaa aatttacaga
      181 aaactttaat gaacaaatcg tatttataga taataagcca caagtaaaac agtctggatt
      241 agaaaaactt cgagaaaatc aattgtggtt gcaaatttta gattctagtg gaaatagagt
      301 ttatgggttt ttagaaccaa aagagcataa agattattat tctaattctg aattattagc
      361 attagctaac caaaataatt caagtaatgg aactgtatac tttaatacaa ttaaaaataa
      421 taaaaatgac tatatttata ttatatattt tccattaaat atatctaaag tgaatatgtt
      481 ttttgatgga ggtaaattta taggtggtaa gtcaattgta ttttcaatca tagggattat
      541 atttttaata attttggttt ctggaattgc ttatggttac tgggtaacaa agtgcatatc
      601 aaatattaca tctgctgtta gcgatatttc aaaacgaagc tatcttccag ttaatattaa
      661 aggtgttttt gctgatatat ataatagttt aaatatttta aatgaagaaa ttcatagaag
      721 tgatgagata caaaataaaa ctgatattat gagagaagag tggatttcaa atataactca
      781 tgatttaaaa actccacttt caccaataaa agggtatgca gagcttctta cagatagtag
      841 tactaattat tcacctgaaa aagtatataa atatgcttca atcatgttaa agaatataga
      901 atatacaaat acacttataa acgatttaaa attaacctat caattaaaaa atggaataat
      961 ccctttaaat aaaaataatt atgatttagt aagatttttg aaagaattat ccattgatat
     1021 tttaaactat ccagaatatg aatcaaatat agttgaattt aagagtaata tagataatat
     1081 taactttgat tttgattgta cactattaaa acgagctttt agtaatctta ttgtcaatgc
     1141 ttttacatat ggagataaaa atactaaagt ttgtgtacat ataacattaa atgaaagaat
     1201 tgaaatcatt atatctgata atggaaaagg gatgtcaaga gaagaagcat caaatttgtt
     1261 taatcgttat tatagaggaa gcaatacaaa acaaaaaata gaaggaactg gtttaggact
     1321 agcaataacg aagcaaatta ttgaagttca tggagggaat atatctgttg aaagtatacc
     1381 tggattaggt agctcatttt atatcacctt tcctatttaa ttaaggttaa ttaaggtcaa
     1441 ataaaaaata tattaaggtt gatagattat tatatttata tagtagttta tcaatctttt
     1501 ttatttaagg ggtgatttga ttaaaaatat tataaataga atgataaaag tatggaggag
     1561 tctattagag atttaattta gattaaagta taaaaatatt agattgaaat atcttagata
     1621 aggaaagaag gtgtaaattt gaaaattata ttaaagtata ttttcacaaa tattaaagaa
     1681 cgaaaaatta gaacagcagt tatgttattg tccatagttt tatcaacagt tttacttttt
     1741 gtttcattct caattggatt atcttatgaa agtgcacaaa gaaaaatggc aaaaggtatg
     1801 tatggtacag cgactatatc tgtccaaagt aaaaatccag atatattaac caatttagaa
     1861 gacatacctg atttaaatgc tataaaatca aaggttggtg tattggaaag ctctgctatt
     1921 tataataaag ggggatacta tgaagaattt agtcttatat cagcagactt aagtcaacta
     1981 aataaaatca acaagccacg attagaaaat ggcgatagta ttactgattt ttctggtgat
     2041 aaaattattc ttccaaatcg ctttacatca aaatataaaa taaaaaaagg tgattctatt
     2101 actttgcaga tttatggaaa gtcttatact tttcaagtta gtgatattgc atcttatgat
     2161 acagtatttt tgaggaacac aagaggtgta aatgcactgc tacccaaaga gactctatca
     2221 aaaattataa ataaaggcag tggatataca agagttctta tagagtcaga agaagaaatt
     2281 acagaaaatc tagtaaataa attatccaaa gaactttcta ctgaaaaata tatggtatca
     2341 aatactataa atgaaactaa gataatttca gatgctagac aaaagacaat gcctttcttt
     2401 ttaataagtt tttttgcatt aacccttagt atatttataa tatacagcag ttataaggtt
     2461 attacattag aacgtcttcc ttttattgga acatttagaa gtattggtgc caatgaaaaa
     2521 actgtaactc gtatattgat gcttgaaagt atattatacg gaagtatagg aggattaatt
     2581 gctattccta ttggtgtagt tgttttaaat ttaatgcttc atgggttagg aaattctctc
     2641 gaacaaggaa tatcaattcc agtagtaata tcacccattg gagtgattat atctgttata
     2701 gtagcaataa tcgtttcttc atttagtgca tatatacctg ttaaaaaagc tagccatttg
     2761 ccgataaaaa atattgtact aggaacagta gaagaaaaga atgtttcaaa tcgttctata
     2821 ctttttatag gaagtataat gtttatttta tctattttac ttccaagaat atcccctgaa
     2881 aatacattgt atctagctgg tggattttca ttactgggat taattgtagc tactattgtg
     2941 ttgattcctc ttattactga tatcatgtct atagtttttg agtttgttta taaaaatatt
     3001 ttagggaatg agggtaaatt agctgctaga aatatgaaaa ataacaaaaa tataatacag
     3061 aatatcactt tactatttat aagtatttct gcagttattg caataagtgt tgttggtaac
     3121 tttgtcaaaa cttatataac agatgtgttt cgtgacgcag aattacaagg ttttgcagat
     3181 ggaaagatga atgaagagtt tattgaagat gttagacata tggatggaat taaaaaaata
     3241 ttacctcttt atgttatgaa taatgagatt tctggtaatg gtgtaacatt atcaagacta
     3301 gagggtacag ataatataaa actatataac tctatgtttg gaatcaatta tacaaatttt
     3361 gagataaaaa aacaagtaat agaagctttt aatgataaac gttctgttat tttgaatgaa
     3421 gatactctaa aaaaagtagg cttatctata ggagatacaa taactctatc taatgataag
     3481 tatgattttt cttataaaat agtaggtagt tttaaatccc gcgcaaatga tgtagaagct
     3541 gttataccat cacattatgc agttaatgat tttgataaaa ctaattatgg ttttttagta
     3601 tatacagcag tcaatccaga tgctgttatg attcagataa gatatttatt tggagataca
     3661 tataattgga gtcgtacagt tgaagaattt aataatgatt ctttaaatac tataagtagt
     3721 tttttgtctc ctatgaataa gatgacctat ttcatttttc ttcttgctac agtaggtatt
     3781 attaataatc tactgattaa ttatattcaa aaaagacgtt ctatagctat gtataaatct
     3841 ataggactaa gtaataaaca aaatataaaa gtgacattga ttgaagggtt tacttcaggt
     3901 ttacttggag ctgttattgg tattgtgata tctatacttg agattcaaac tatctttata
     3961 gttgcaggac caaaaatttc aatgaaacca gatttagatt ttaaaacatt tattattgtt
     4021 gggttattgg gaattatagt aactttaata ggttcgatag tgcctattat aaaagggaag
     4081 aaaatgaaac tgatagaaga aattaagttt gaataattat ggaggttaat atgaaaatta
     4141 cacataatag tttagctgta gaagcaaaaa atattatcaa agaatataaa attggaaata
     4201 caacaactcg agtgttaaaa gaggtatctt tacaagtaat gaaaggtgaa tttgtttcta
     4261 tcatgggtca atctggttct ggcaaaagta ctcttttata tattttaggt ggtttagata
     4321 ctccaacgag cggaaaagtg tatatgaatg gagctgatat atcacatttt aatgatgaga
     4381 aaatgagcat aatacgtcgt agaaatatag gattcgtatt tcaattttat aacttaatac
     4441 caaatttaaa tgtggaggaa aatataatgc ttcctttgtt attagatggt aaaaatttaa
     4501 aagattataa aaatcaatta gatgaaatct tagatatagt tggtttaact gatagacgta
     4561 aacatacacc acgagagtta tctggtggtc agcaacaacg tgttgctata gctcgagctt
     4621 taattggaaa accagagatt ctatttgctg atgagcctac tggaaattta gatagtaaaa
     4681 cagggataga aataatagat ttactgaata aaataaacag agacaatgga caaactataa
     4741 ttatggtaac tcactcacca gaagcagcca aaagtagtag tcgtactatt acagtttcag
     4801 atggtttaat tgtataagga ctaattattg tatttgagat aagcattttt aattatataa
     4861 attagacata tgtaaagtgg atataagtta aatactataa atgtgctata atattaaaat
     4921 aacagataaa agtactatat tttttattga ttattttaat tttatttgtt tattaaatat
     4981 ctaatgagta ttcatactat ctctattatt aaaagcagtt aatgatatat agagataata
     5041 tggattcttc ttactttaat aaatattcac gataggacag gatataatat atgttaaaaa
     5101 aagaaagtat tcattttata aataaaaata ggtcacttat agtttgtgaa gacaattttg
     5161 tttatctctt gccttgtata caattaagaa attggatatc gaattttact atatcgtttc
     5221 caaatcaaac aataatttct gataattaca cgattattcc tcatggaagt gtaactttgg
     5281 tgttttttta tgatgccaca gggttacata gtctattatt tggtccaact actaaaccaa
     5341 aaacagtagg aaatattgct aatagatgtg atgttatact aattatagaa tttcaacctg
     5401 ctggtttttt cccgcttata ggtatacagc aaagtgaact tattgataaa gttgttccat
     5461 tttctataat taatact
//
LOCUS       DBGJFU010000183         5421 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.187, whole genome shotgun sequence.
ACCESSION   DBGJFU010000183 DBGJFU010000000
VERSION     DBGJFU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.187"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(223..744)
                     /locus_tag="ACOIAS_003517"
     CDS             complement(223..744)
                     /locus_tag="ACOIAS_003517"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2703185.1"
                     /translation="MKFKRLRTSENKLCYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            1100..1897
                     /locus_tag="ACOIAS_003518"
     CDS             1100..1897
                     /locus_tag="ACOIAS_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGZ2703186.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            2007..2597
                     /locus_tag="ACOIAS_003519"
     CDS             2007..2597
                     /locus_tag="ACOIAS_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HGZ2703187.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            2663..4360
                     /locus_tag="ACOIAS_003520"
     CDS             2663..4360
                     /locus_tag="ACOIAS_003520"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGZ2703188.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            4574..5413
                     /locus_tag="ACOIAS_003521"
     CDS             4574..5413
                     /locus_tag="ACOIAS_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HGZ2703189.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         2112 a          564 c          907 g         1838 t
ORIGIN      
        1 ataaaatgta attttgtcta taaaatctca cttgttttct taactttaag cgtgaaaaag
       61 agaatgtatc atgaattttt tagttcatga tgcatccttc tttgtatgaa atccaatata
      121 tctttattat ttaaacaatg gaaaagagcc ttatttctat tttgagacat ctcttttatt
      181 taattatgaa gttttataca gttcaataaa taggaattat atttagaaac tcatgacatt
      241 atttcttaga tagtgattaa atttatcata ttccatttta gataactttt ctgggtaaga
      301 cattacaatc aggttatgcc cattaaattt ttcatggtat ggtggatgaa tgtgttcata
      361 attatttgtc ttatatttta ctctttcata gaacgccttg cgataaattg aaatctcatc
      421 aattggaagg tcaatttcta aaataatagt ttttcctttc ttatttaaaa gctctaacgc
      481 tctttgtcca tatctattat tgcgcatttc tggaaaaatg cagaaatgtt ctacatagat
      541 aaagtcatct gtttcccaac ataaaattat gccgacaaac tggtttttat cataaattaa
      601 attgaactga tattctttat ttttcaaaat ttcttcttgc aaagaagatt ttctttgttc
      661 atgaaatggg aaactaattt tatataattc catagccttg taacataatt tattttcact
      721 ggttcttaac cgtttaaatt tcactttact cctcctaatc acgaaaattc caatttattt
      781 atgagttcat tttaccatag tcagtatttg catacaatgt gttttaagtt gagtgtcatt
      841 tctttctttt atttatgctt atatctcata gactaagaga taattgctta agacataact
      901 aaaaactatt atattaagct ttgataatct aaaaacagta tatttatgga cttttctagg
      961 tctaaaagta atcttaataa ttttagaagt tacaaaaaag ttacataaat ttggaaagct
     1021 ataatataca tatataatat aaggttaaaa ctgtaatgaa taatattaat ttaaaatttg
     1081 aacatggaag gagattatta tggagaaaag agaaaagagg ataataatat catctttatt
     1141 aagtgtatca attttaatgg gtttggtaag tatatattct atattaaata aagaagacat
     1201 aattttaact gttaagggtc aagagcaaaa agtatcttcc tttaagaaaa cagttgagga
     1261 acttttggat gaacagggta taaagtataa ttctgaagat aagattaatc caagtttaga
     1321 tacagaacta aaagatgata tgaaaataaa agttgttaag gtaactaaaa gtaaaaaaga
     1381 agagattgaa aaaattccat ttgatacaaa gcatgtaaat gatagtaatt tgttaaaagg
     1441 aaaatctaaa gtttatcaag aaggtcaaga aggagaaaaa aaactagtct ataatttaac
     1501 ttaccatgat ggaaagttag tcaaaaaagt cttatcaaaa gaagtaatat ctaaggagcc
     1561 aactacaaaa attataaaat atggaactaa agaaaaagta ctaatagcat caagaggagc
     1621 aaatataaga ggaggcaaac atatgaaagt ggttgccact gcatatgcag gagatacaat
     1681 aacatctact ggtacaactc caagatgggg tgttattgca gttgacccac gtgtaatacc
     1741 atatggaaca aaagtatata tacctaaact gggtatgact tttgtagcag aagattgtgg
     1801 aggtgcaatt aaaggtaata gaatagacat ttttatgaac agtgaaggaa aagcttcaaa
     1861 ttggggaagg aaaagtatag atatatatct acattaaatt agtgaataga ataactttac
     1921 ttgaaggaca aaaaagtata aaatatgaat taactaaact gttagattat tcttaaaaca
     1981 taaaaatata tctaaggggg gataagatga aaatagtatg cttaggagac agtttaacgt
     2041 atggatttgg agtatcacga agtaattcat ggacaaacat tgtaaataaa gagactcgat
     2101 tagaaatagt aaataagggt ataaatggtg atacaacaag tggtatgtta gctaggttta
     2161 atgaagatgt tgtaaaaaat tctcctgata tagtttttat aatgggtggc accaatgatt
     2221 ttattgctgg tgcgggaaat gaaattatta attctaatat aatggctatg gtgcatcagt
     2281 catattcaaa aaatattata cctataatag gtataccatt aaaaccagat attccaaatg
     2341 ttagggaaga ttggagttgt tttactgatt ttaatatagt atctcaaaac ttagaatcat
     2401 atagttattg gataaaaaaa ttttgtatga catttaatac taatttcgta gatttttatt
     2461 cagaatataa taaaaatatg gaactagaag gatataaaaa attatatttt gatgggttac
     2521 atccaaccaa agagggtcat agaataatgg cagacatttt tattaattca attaataagt
     2581 atatagaaga gttatagatt acattaaaat gcttgtttat gcgtcagttg caataaatac
     2641 atacagtaga gaggggttta ttatggatta tactaaaact tatgaagagt ggataaaggg
     2701 ttcatatttt gatgaagata caaagctaga gttggagaat ataaaaaata atgaaaaaga
     2761 aatagaggat agattctata aggatttaga atttgggact gcgggtctta gaggaataat
     2821 tgaagcagga acgaatagaa taaataagta cacagttaga agagctactt ttggattggc
     2881 aaactatata ttagaaaaca caacaaaaga agaaacaagt agaggagtag taatagctca
     2941 tgataataga cacaagtcta gacaattctg tatagaatct gcaaatactt tggcagcatg
     3001 tggtataaaa gcatatatat ttgatagttt gagaactact ccagaattat cttttgctgt
     3061 tagaagctta aatgcaatcg ctggtatagt cataacagca agccataatc ctccagaata
     3121 taatggatat aaggtatact gggaagatgg agcacaagtt atgccagaga ttgcaaatgc
     3181 aataacagag aaagtaaata gtatacatga ttatagtaca ataccaacct taacggaagc
     3241 taataaaaat ttggtagttt tgttggacga atcacaagat actaaattta tagaagcagt
     3301 gaaaagccaa attataagaa aagatttagt taaaaatgta ggtaaaagct ttaagatagt
     3361 ttatacacct ctttgtggaa cagggaatgt tccaataaga agagcattga aagaagttgg
     3421 ttttgaaaat ataattgtag ttccagaaga agaaaatcca gacccaaatt ttgcagggct
     3481 tgattatcca aatccagaag agaaaaaagc tttaaataga ggtatattac tcgcaaaaga
     3541 aaaaggagca gaccttgtaa tagcaactga cccagattgt gatagagtag gtgtagcagt
     3601 aaaaactact acaggtgagt atgctttact tacaggaaat caaattggtg gtatgctgac
     3661 acattatata atagaaggat taaaagaaaa taataaatta aaagaaaatc ctacaataat
     3721 aaaaaccata gttacatcag aatttggagc agatatagct aaagcaaata atgttgatgt
     3781 attaaatgtt cttacaggtt ttaaatttat tggtgaaaaa ataaaattat ttgaacaaaa
     3841 taaaaataga agttatgtat ttggatatga agaaagttat ggttacttag ttgggactca
     3901 tgctagagat aaagatggtg tagtttcttc attgttaata tctgaaatgg cagcatttta
     3961 ctattcaaaa ggtatgagtt tatatgatgg tttaatagaa ctttataaaa agtatggatt
     4021 tttcaaagaa cagactatat ctttgacttt aaaaggtata gaaggtgtag aaaagataaa
     4081 agaaataata tcttatttta gagaaaatca aattgattat ataaatagta ttaaagtagt
     4141 tgataaaaaa gattataaaa atggtataga taatcttcct aaatctgatg ttttaaaata
     4201 ctttttggaa gatgaatctt gggtagctat aagaccatct ggaacagaac caaaacttaa
     4261 attttacata gctgtaaaag gagctagtga tattgaagca gataaaaaga ttcaaggttt
     4321 gaaaaagtat atagatgaca tggtcgaaaa attaaaataa aatattatta ttttataaga
     4381 taattttgta ataatattta tttagctgaa aattataatt tactttcatt gagtagaata
     4441 tggaaatgtt agtagaattg ttattttcag cttttatttg tgttattttt acttaaataa
     4501 ttatattata gaatgaatct gagtataatt aatattgtat aatgaaatta atttgataga
     4561 aaaggggata tttatgtttg gtatagataa gaaattgatt gaatatataa ttgcaaaatc
     4621 caattacagt gaattaagtt caaagtcagc agaagtatct ttcttcttaa tgttgtccat
     4681 atttcctttt ttaatattta caataagttc tatagcttat ataccgattc ttcatttaaa
     4741 taagtatata gctttgttta gaaatatgat gccagaaggt gcttttgctg tgttatcttc
     4801 aatcatagtt tctgcaatag acaatagaaa cctgaaattt ttagcagtca gttttgtact
     4861 tactatgtgg acattttcaa gagcagtaaa agctcttatt aaaggaatga atagagccta
     4921 taaagttaaa gaaactaggt ctttttttaa aattttatct atatcatttt tatttacaat
     4981 aatgcttttg gtccttattt ttttatctat gatattttta gtttacggtg aaaaaatagg
     5041 ttattttatt tttaatcttg taggattgga tgagatattt ataaaaatat gggatattct
     5101 aagatacact gtaggtataa taacaataat agtgatattt acacttttat ataaatacac
     5161 tccaaataaa aagttaacta ttaaagaatc tgcgccaggt gctatttttg ctacttttgc
     5221 ttggttttta gtatcatttt tatattctta ttatacaaat tactatgcta actatgaagt
     5281 aatctatgga agtatagctg aaataattgt acttatgacc tggatgtatt ttagtagttg
     5341 gtctatcgtt attggctatg aggtaaattc aagattgtat tttagaaaga taagacatga
     5401 aatgttaaag taaagtattt a
//
LOCUS       DBGJFU010000184         5403 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.188, whole genome shotgun sequence.
ACCESSION   DBGJFU010000184 DBGJFU010000000
VERSION     DBGJFU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.188"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            267..1730
                     /locus_tag="ACOIAS_003522"
     CDS             267..1730
                     /locus_tag="ACOIAS_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGZ2703190.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1786..2910
                     /locus_tag="ACOIAS_003523"
     CDS             1786..2910
                     /locus_tag="ACOIAS_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2703191.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKK"
     gene            3157..4272
                     /locus_tag="ACOIAS_003524"
     CDS             3157..4272
                     /locus_tag="ACOIAS_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HGZ2703192.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4424..5293
                     /locus_tag="ACOIAS_003525"
     CDS             4424..5293
                     /locus_tag="ACOIAS_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HGZ2703193.1"
                     /translation="MDYLKYFKKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2077 a          550 c         1008 g         1768 t
ORIGIN      
        1 ttcaggcttt tcattatatg tattccaatt tatattgttt gattattatt caaacacacg
       61 tcgttaaaaa ttttataaat ttatgtttag atatgattaa tactcttgat tttagaaata
      121 aatatttgta gattgtagat taaagataat ataaataaat agaaggtaga ttttttagat
      181 atattaatat aaagattata taaaatattg aaatatagct gtttaaatta ttgtctataa
      241 atataaaatg aatggtggtg agttaagtga ccgatttagt taacaccatt aatggaattg
      301 tttggtcacc cgtattagtt gcaatgtgtt tgtgtatagg attatatttt tcattgaaat
      361 taaaattttt tcaaataaga gatgtaaaag aaatgtttac tctcttgctt gaaggagata
      421 gttctcatgt tggtatatca tcatttcaag gttttgctac atctatggct ggaagaatag
      481 ggactggaaa tatagctggt gtagctgtag caatagcaat gggaggtcca ggagcattag
      541 tatggatgtg tataatggct acattaggtt cagcaactgc atttgtagaa tctactctag
      601 ctcaaatata taaagatgag catgatgggc agtatagagg aggacctcca tattattttg
      661 aaaaaggtct tgggtggaaa aagtgttcag ttttattttc aatagttgca ataataagtt
      721 atacattttt tatgcctggt acacaatcaa acactttata tctagcaatg aatcaagcat
      781 ttggattttc aaaaatgaca atagcagtag catcttctat tgcattagcg ataataattt
      841 ttggaggaat taaaaggatt ggtttatttg ctgaaaaagt agtacctata atgggtggag
      901 cataccttat tatgacatta gtaataatta taacaaatat tagtttagtt ccagaggcta
      961 ttgtaacaat gtttaaatct gcatttggat ttggttcagt ttttggagga atgctaggtt
     1021 cagcaatatc ttggggagta aaaagaggta tatactcaaa tgaagcagga gaaggttctg
     1081 ggacttatgg tgcggcagca gcagaagtga gtcatccagc caaacaagga ttagttcaag
     1141 cattttctgt ttatgtagac acattattaa tatgtatggc tacaggtatt atgattgtaa
     1201 taacaggaat gtataatgtt acaggtgttt gcacagcact gccaggtgta gaaccgggac
     1261 cacaatatgt acagagtgca ataaatacat tacatccaca attaggtgat atttttatag
     1321 caatagtttt attattattt acatttacaa ctttattagc cttctcatat atgatggaga
     1381 ctaatgtatc ttacttagta actagaatta aagcagaaaa acatgaaaag tacataatta
     1441 atgtctgtag atttatgctt atagcatgtg cagcaatgtg ttgttttata gaaccactta
     1501 caagctgggc attaggtgat atatgtatag gtgtattaac atatataaat ttaattgcca
     1561 tctttacact aaaaaaacca gctataaagg catattatga ttatataaga cagaagaaga
     1621 tgggaataaa aaggagtgat agaactttta accctataga gttagatata agaaatgctg
     1681 agttttggga aaatagatac ttaaaagagg ataagcatga gttaaattaa agttataata
     1741 aaatataaaa attactaaat aaatttagtg ggaggtaaga ttggtatgaa agaaagaata
     1801 atacaagtgg ctgattcaaa gaaagaaaaa attctagggc tatgtcaatc tttatatgat
     1861 gaaccagaaa ttgctttgca agaatataaa tctgctaaaa aaatatcaga atttttaaga
     1921 gaagagggtt ttgatgttga ggaaaacttg gcaggtatgg ctacagcttt taaagcaacc
     1981 aagaaaaatg gagatggtcc taaaatagca tttatagcag aatatgatgc tcttccaggc
     2041 aatggacatg catgtggaca tcatttaatt gcatctatgg gagttggagc tggaatagct
     2101 ctttcaagta tactggatac ttacaaggga gaagtaagta taattggaac acctgctgaa
     2161 gaaactggag atggaaagcc ttacttgata gaacatggtg tatttgatgg ctatgatgca
     2221 gcaatgatga tacatccaaa ttcaaaaact tgtgtaacac ctgaaattat agcaatagga
     2281 ggtctagatt tcatatttac tggaaaagca tctcatgcag gagctaagcc atataacgga
     2341 ataaatgctt tagatgcagt agtattacta tataataata taaatgcact aagacaacaa
     2401 ttagtagatg gaacgagaat acatggtatt atactagaag caggaactgc tgcaaatgta
     2461 atacctgata tgggaaaagt aagattagag ataagggcaa aagaacaaaa ttactttgat
     2521 gaagtagtag aaaaagtgaa aaattgtgct agaggtgctg caatagccac tggatgtgaa
     2581 cttgaatttt atcactttga accaacttgc caaggattaa atgaaaataa agttttggta
     2641 gacatattta caaagattat ggaggaattt ggaatatatg aagatgaaca agtaatgtta
     2701 ggttcaacag atatgggaaa tttaagtcaa ataatgccat gtatacatcc tttgatgaag
     2761 ttttctgaaa atggagaaga actacataca aaagaatttt tggaggcttc tataaattca
     2821 tatgcaaaag atagagtaat tgatggtata aaaatattag ctttaacagg atttaattta
     2881 tttgaaaatc cagagttatt gaaaaaatga aggaagagta gaacttagac tatttttaat
     2941 tatactcttt aaaataagaa gagtgtctca aaatgaactt tttagtttat gagacactct
     3001 tttttgtatg aaattaaata tatttttatt attttaacaa tgaaaaagag gcttatctgt
     3061 ttttatatga ccttttttaa cttgtagcac aaaatatcat gaatgattac aattcgaatg
     3121 ataaaatcga ttataagata tataggaggg gtacatatgg aaactttaga agtaagaaat
     3181 attgaattga gtggtacaaa ttatgagata ggatacagac taggagaatt ggttgctaat
     3241 atgccagaga taatagaagg tcagattaat aaatctaatc tggtaagcaa aaaagaagaa
     3301 aaagagatga tagagttgtt tgataaatat tgtccaggtc ttaatgaaga attgcaaggt
     3361 tttgctgatg caattcaagt gaattgtaat caaattttat attatactat gacttatctt
     3421 aaaccaggtt gtagtcaagt tgcattagca ccagaattaa ctgaaaatgg gcatgtacta
     3481 tttgcacgta atttcgattt ctcacacaat atggaggatt ttgttttatg taaaactaag
     3541 gtaaatggaa aatatgcaca tataggtact accgttatgc aatttggaag aggtgaaggt
     3601 atgaatgaat gtggcttagg tgtaagtcag tcatcatgtg gtattcctgt aggcaattca
     3661 gatggtttaa gaaaaccagc aattgtaggt ttacagtttt gggctgttat tagatatttg
     3721 ttagaaaatt gcaaagatgt agatgaagca ttagaatatt taaaagatat gccaattgca
     3781 tacaatatta atttattgct tgcagataag tctggaaaca ttgcacttgt ggagacttta
     3841 gatgggaaaa aagaagtaaa tataatcaat agtttagaaa gtaaaagaga atatttttta
     3901 cactctacta atcatacaca tattgataaa cttcataaat tagagccaca atcaatgaaa
     3961 aattctattc atagatataa attaattaaa gaatatataa ataagtctaa aaaaattgga
     4021 gagaaagagt taatgaattt attgtcttca aaatatccaa atggcttatc ttgtaattac
     4081 tataatgatt tttttggaac tttaaaaagt atagttatgg atttaaatat aggaaagttt
     4141 aatattttat ggggtggact agagaataaa tgggagagct actatttaaa aaatgacatt
     4201 aaatctataa cacagaggat aaacataaat attgaaaaag caccatctga cttttttgat
     4261 ttcattgaat aaaaaatgta tttaattatt ctaaataatt aggatatata aatcaactaa
     4321 aagtttttat ttatcgtatt gaattagtta taatatttta gtatagtaaa aaatagtgct
     4381 attttacata tttaaagagt ttaagtagaa gagaggtgcc atattggatt acttaaagta
     4441 ttttaaaaaa gctgtaatgt atattgagaa taatttacat gaaaatataa ctgtaaatga
     4501 tgtggcaaaa gaaactggat attcttatta tcatttaaca cgcttattta agtctatgtt
     4561 tggtgagagt gtaggaagtt atataaaaaa aagacgatta gtaagtagta caaaagaact
     4621 tttatatagt gataaaaaag tgatagatat agctattggt agtggatttg aatcttcgga
     4681 agcatttagc agagcattta aatctatata caaggttagc cctatagaat atagaaaaaa
     4741 tagaattgat gtatttgttg gtaaaaagaa aaaattagag ttagatttta tgagacatct
     4801 tgttggaaat ataacgatta aaccagttat aaaagaaata gaaaaaataa aggtaatagg
     4861 tataaaggat aaagtaattt tagaaaataa ttcattacct gacttgtggg agaaattcag
     4921 aaaagtacat catattgtcc caaatacttt gccatcaaag agagtttttg gaatctgtga
     4981 agcaatctct gaaatacacc tagttagtga aagtatggaa tttaatgaga taataggact
     5041 tgaagttaat agttatgatg tgattccaaa ttcatttgtt tcaaaaacta ttaaggaagg
     5101 aaagtatgct atatttactc atactggtag cttagataat ttagacaaaa cttatgaata
     5161 tatatggggt acttggtttt taagttcaaa agaagaactt gatataagag atgattttga
     5221 agtatatgat gaacgatttt taggtcctag taatgtgaac tcacaaattg atatttatat
     5281 acctataaaa taatctataa gaaaactaaa aaataatttg attaagaaat agatgttcgt
     5341 aaaagagcat ctatttttta tttttatcag atttagataa ttttaataat aaaagaaata
     5401 aaa
//
LOCUS       DBGJFU010000185         5380 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.189, whole genome shotgun sequence.
ACCESSION   DBGJFU010000185 DBGJFU010000000
VERSION     DBGJFU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.189"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(1499..1717)
                     /locus_tag="ACOIAS_003526"
     CDS             complement(1499..1717)
                     /locus_tag="ACOIAS_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2703194.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(1718..2125)
                     /locus_tag="ACOIAS_003527"
     CDS             complement(1718..2125)
                     /locus_tag="ACOIAS_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703195.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(2211..2699)
                     /locus_tag="ACOIAS_003528"
     CDS             complement(2211..2699)
                     /locus_tag="ACOIAS_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HGZ2703196.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(2904..4025)
                     /locus_tag="ACOIAS_003529"
     CDS             complement(2904..4025)
                     /locus_tag="ACOIAS_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGZ2703197.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(4398..4568)
                     /locus_tag="ACOIAS_003530"
     CDS             complement(4398..4568)
                     /locus_tag="ACOIAS_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703198.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(4740..5183)
                     /locus_tag="ACOIAS_003531"
     CDS             complement(4740..5183)
                     /locus_tag="ACOIAS_003531"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2703199.1"
                     /translation="MIEIKTIEINKKSQIDLLILADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1818 a          847 c          464 g         2251 t
ORIGIN      
        1 aaaaagaaac cacattctag ttttatacag aacatagttt ctttaaaaat aatcaaattt
       61 ggattttaaa taattaaaaa caaactttta tttgttttta attttccaat tttatagtag
      121 ataatttagt taattagttc ttaaatgttt atcttcttta gtaaattttt ttcttattaa
      181 gaaatatgca actattacaa caagttctat tgctaaaaat acaccataag tagctataga
      241 atctatgtat ctatgccatt tctaaagaaa attaaagcaa ttcctgctgc tataatacct
      301 acagcatttt aattttgacc aaaaccataa aaagaaggga aatttccttt tatttcaatg
      361 aatgtcaaga ttaatccaat agctataaat agtactccta caacttccat aatcatgcta
      421 ggcattttga cccctcctta ttaataatta actactacta taaaaatatt tattattttt
      481 ataattatat tatatatgcg attacatgtt tttggaagta tatttggaag taatttcata
      541 tttatttaac ttaatttttt tgtgataaaa taattctaat tttcatatag atattattat
      601 aaattttcta gtttatggag ttattcattc tataattcac aaatatgtat tttttaatct
      661 attaaactat tcattttctg gcttataagc atggacttct tatctataag cttggttttt
      721 catctaataa gttatttatt ctttaatcta tgagcttgat ttttcatcta taagtcattt
      781 attttttaat ctataagttt agttcttcat ctataaatca tttatttttt aatctataag
      841 tttggttttt catctataaa taagtcattt attttttaat ctataagttt agttcttcat
      901 ctataaatca ttcatttttt aatctataag cttggttttt acctataatt aattcattct
      961 ctaatctata agcttggttt ttatttataa gtcatttatt ctctaatcta taagcttggt
     1021 ttttatttat aagtcattta ttctctaatc tataagctta cttcttaatc tataagtcat
     1081 ttattctcta atctataagc ttacttctta atctataaat cattcatttt ttaatctatt
     1141 agcttgtttt ttacctataa ttaattcatt ctctaatcta taagctttct ttttcatcta
     1201 taaatcattc attctttaat ctatgagctt ggtttttcat ctataagtca tttattctct
     1261 aatctataag ctttcttttt catctataaa tcattcattc tttaatccat tagcttggtt
     1321 tttcatctat aagtcattta ttctctaatc tataagctta cttcttaatc tataaatcat
     1381 tcactcttta atccattagc ttggtttttc atctataagt catttattct ctaatctata
     1441 agcttggttt tttacctata attaattcat tctctaatgc ataagtaata catgaaattt
     1501 aaatattatt ttcttcaata aattcaaaca actcttctac agttgtatca aataccttag
     1561 caatatccat tgccagtttt aaagaaggat tgtagcgacc attttctaaa tgtacaatag
     1621 tttcacgtct tactcctact gactttgcaa gttcaccttg tgtcataagg ttcttttctc
     1681 tataggattt aatttttgtc tttagtaata ctgacaccta attgtatatc ccctttctgt
     1741 caaaatatat aaatagaatt aacttcaaca aggattgaat aaacaaaatg actaataaag
     1801 acattcctat atttaaattt gacacattta tgtccaaagt tacaagaaat atagatacta
     1861 tagctacaga aaaaaacaat acttttattg caatatcatt aactcttgat agtatacatt
     1921 ttgatgcctc atcaattaaa tcatcatact ttttattata gacccactga gctcccatag
     1981 ctaataccca aataattata ggaagatatt caatactact tcctttaaaa aaatacttat
     2041 ttactaatgt agctccaata caataagaaa ttcccaatat ttctgaaata agtgttgatt
     2101 taataccagt ttgttgttta gacatataaa atccccctta aaaagttatg tatttataac
     2161 acctcttgtt ataaatatat aacttttttg aataaatttc aataaaaata ttattttgca
     2221 acaaattctt ttattaattt tacattagat agaggcactt tagatgtcac tccttgcaat
     2281 gtcaaatctt ctatattaac ttcatttaat tttgaacaca gttctaagtc aaatattctt
     2341 tcccaactta ccttcttttc aatttgttca tcttcataaa gctctacagt aatatccatt
     2401 aaaggtaaat gccataaatc aaattctgat aataacacat tgtcttcact tatttctatt
     2461 tcaagtaaaa cacctttttt tcctttttct aaataaccac tacatcttaa atcaggtctc
     2521 ttcgtccaaa cccatatcat actatcatca aattcatata ttctatttgt catttgacta
     2581 tacatccatt tgtatggaat atcaaagtct tctaatataa aagaataatc atttgataga
     2641 tatttattgt ttttattgtt attccaaaat tctaaagtct gtatagtata aaatttcata
     2701 tataaatcaa cccctttgtt tttctataag aaggagatgg ttcaaacttt tattttgaat
     2761 tatctccttt atttatacac ctctttgtac ctattattta tatatctctt tatatctacc
     2821 atatttaaat aacaaagttt tatattaatt taaatttaaa agaggaagct ttgtatttat
     2881 ctagtgccat ctcaatcata gtattagttt tgattataga ctttttcaac ctctgattta
     2941 ttcaaatcat ctataatatc gcatatttca ctcaaattgt atcctttatt ttttaatttt
     3001 atgatgatat taaatttctc ttctcttttc ccttttgcaa tacctttttc gatacctttt
     3061 tcgatacctt tttcaatacc ttcttgaaaa atttcttctt taaaatttct acttttattc
     3121 ataatatacg cacctctctt taaatttaat aaatagtcct cattactacc taattctttt
     3181 aatctatcaa gtgctctttt aataacataa tccgttttag atagtattct aatatctata
     3241 tgagaaggat tatcaataag tttaccccat attagaactc tgtttgctat acaactggat
     3301 ttacgaattt ttggaagttc tataaaatat aatgcagttt tactagaaaa aatatctgga
     3361 tgcccgtcca ttgttaatct caggcaattt acataatctt ctatgtgatt atattttaca
     3421 tgatttagta tatgtattcc aacagttttt ccaatttctt tatattcttt accttcttct
     3481 aactttctac catgtacacg actaagataa tattcagctc tttccaaata atcctcttca
     3541 agagctcttt gcatctcaat atttatatgg ttacgttctt catcttcagc caaaatatca
     3601 agtctaggtc ttttctcacc aatgtaatta attgttgggt cggtttcaac cttaattatt
     3661 ctttttattt gagtgaactg tttgatattc ctattagcat atggagcatc tttgaggata
     3721 tcatttataa aatttgcaag taaatcgtaa tcattattta aagtaaacaa tgatttaaat
     3781 acctcatcat actcaggctt agcataccca tcttcagtta caacttctcc aacattttca
     3841 ctagtcaata tttcttttgg aattaaatcc ttgattttat ctaatttttc attagaaaaa
     3901 tcacctctac taagtatgtc atttaatttt tgggtccatt ctctgtcctt ttcttcaatt
     3961 atagttcgtt tatactcttt tgatgtttta ataatcttag atttttcttc taatttttct
     4021 ttcatatcaa cacctctttt ttattattac ccatttcttc ataccaaagt ctgaacttct
     4081 aagtaaaatt tgcattattg aaataagaac cttataaact tatatatcat tactaaagtt
     4141 aagactttaa agagaagcac ttagattaca atagatttta gatagctttc tttttaaaac
     4201 tattgttgat aaatgaatga atataagggt atgtattaaa atagagatac tcatcttttt
     4261 cattgtttaa gtaatgaaaa tatattttat ttaatacaaa aaagagtgcc ttataaacta
     4321 aaagttcact tcgagacact cccttacatt ttgtttctta aactttacaa tattacatac
     4381 ttatttatta atttaatcta tgcatactta acccaataat aaagtacata tatccaactc
     4441 attaagccat gaaaaatagc ccatggaata gatttccatg cagtataaga aataatcatt
     4501 gctaaacaag aaccaaaaga aatacccttt ttaacagttg attttatagt attattattt
     4561 gaatccataa ttaatcctcc aaaattcaaa ataaacaagt tattatatat cttatttgaa
     4621 tctaatacaa tataacagct tctaaataaa ttatactctt attatacata atttataaat
     4681 tataattccg ataattatat gaattaatta attatattaa cctaaaaata ttatcctatc
     4741 taaaagttat atgatagata caacatgtca acacattgta attctccttc aaagattggt
     4801 tctggataat tatctacaaa aaagttttta attttatgtg aataaacaaa tccaaacttc
     4861 tcataaaaag gaactccact ttcagatgta ccaacaacca ttgatgaata ttttttacta
     4921 taaacatcta aaagatgata aatcatcttt gaggcataac ccatattttg aaaatgctca
     4981 tatgtagcaa tgttttttaa ttcacatatt tctttggaaa tttcatttac aactgctata
     5041 caagtaagtt ctttattatc atacaatgcg tacatagttc ctttattgag atatttatca
     5101 atcatttctt cacatgggtc agctaatatt aaaaggtcaa tttgactttt tttatttatt
     5161 tctatagttt ttatttctat cataagttct ccaccctcac tttattcaaa ccttaattta
     5221 acaaacttct tacagtactt atttagttca ttttgatata tatttttact ttttaaaata
     5281 tagtgataat tccctagaat attaatatta ataataaatt ataatatcag ctctagcaat
     5341 tacctagaat atcaatacta atattcatct ataatattag
//
LOCUS       DBGJFU010000186         5362 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.190, whole genome shotgun sequence.
ACCESSION   DBGJFU010000186 DBGJFU010000000
VERSION     DBGJFU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.190"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            235..969
                     /locus_tag="ACOIAS_003532"
     CDS             235..969
                     /locus_tag="ACOIAS_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2703200.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     regulatory      1424..1500
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1765..3021
                     /locus_tag="ACOIAS_003533"
     CDS             1765..3021
                     /locus_tag="ACOIAS_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGZ2703201.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            3084..4277
                     /locus_tag="ACOIAS_003534"
     CDS             3084..4277
                     /locus_tag="ACOIAS_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGZ2703202.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGIYLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            4478..4858
                     /locus_tag="ACOIAS_003535"
     CDS             4478..4858
                     /locus_tag="ACOIAS_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2703203.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         1974 a          546 c          926 g         1916 t
ORIGIN      
        1 aatagttatt agaaaaaaat aaaagtaatt gcaaagcttg caattactta cttctatatt
       61 aattactata ccataaaata acaaaaaaat caagtcgagt aatccttcaa cttaaatgat
      121 atactaggta ttaataattt ttaattaata aaatataact agaggaaaat ctacattttc
      181 tcaatttctt taattaaatt gacctctaat taagtaatta taaggagaaa aattatgaat
      241 ttagcaaagg gattagatgt tttaaaaatt tcatcaaatg tattcggtga agataaagtt
      301 atgtatattc ctgtaatcta cacagaagat gatgcaacac ttattgacac aggtcttccg
      361 ggtcaaggtg atttaataat tgatgctctt aataaaagta acacgagctt tgataagtta
      421 aaaaatataa taatcactca tcatgatata gaccatatag gaaacataaa ttatctaaga
      481 gaaaaatcta agaataatat caaagtatat gcatacaaaa gtgaaatcag ctatataaca
      541 ggagaagaaa ctccttttaa actttatatg ttggaacaaa tggtagataa aattgatgat
      601 aagatgctta gtatgctaaa tgttatggga cttggattta aatcatcata tactaaagta
      661 gatgtgtcat tagataacca tgaaaaattg aatctaggtg aggaaataga agttatacat
      721 acaggaggtc acactagagg tcacatatgt ctttatttaa aagagtctaa agtacttatt
      781 gctggagatt tgttacaggt agaaaatgga gaacttaagc ctgttgatgt gatgtacagc
      841 aacaaacaag agttaaaaga tgctattaaa aatataagta attatgatat agaaacgata
      901 gtttttagtc atggtggatt atatcaaaga aacataattg gaacattgaa aaacctaatt
      961 attgagtagt actatatgat ttacaataat ttcttaaatt acttaaaatt atatctaaag
     1021 atgtagaatc aaaattaaat ataagtaata agaattttgc tttaaaatta tacttaagtg
     1081 ttaagggtga tatgtagtat tacaagtatt ttagaatata attttttata gtaattttaa
     1141 agttctacaa tacttcagtt gaaaaatatt taacagcata gtataatagg aaagaaaata
     1201 ttttaaataa actttagtat acaaagtgaa gaaaaatatt taaatcatgt atcataatgc
     1261 aaagtaaaga aaatcattta aattatatat cataaaaata atatgaagta ttgaacactc
     1321 ataatattag agactttaat attttaattg ataggttata ttatttataa cattaaaaat
     1381 acaatttaca taaaatagac atatgaaaaa taaaaaatta tataataatt atgggaatga
     1441 ggttctccct tggatgataa tatcctaaac tgccaatgag ctgatgactt ctatgatatc
     1501 atagatgcca ccagcttttt ttgttgttta aatcgataaa atggaggtga agtacttact
     1561 aaatttttat tttttaaatt tgaagaaata tttaattaat agaaaaaata gtagattata
     1621 tgttaattat tttattaaga atgggttaag agtgtaccta tatcctatca atttaaaaaa
     1681 caattatgat agtcattaca ttgactacag ttgatgtaat aaaactttca aaaaatctta
     1741 tgatgcaaat agaaaggaat taaaatgata gaaaaattta aaactttaat atggattaaa
     1801 atgataataa gaattaaaag cgtttataaa acatatggtg gactattttt tttaggactc
     1861 ataggtatac cagttggtgc aattataggt cttatagata ccatatttgg aacggttctt
     1921 ctaaaagtta cagatattcg agaaacatat ccaatgtatc tgataccatt tttagcagta
     1981 gtaggagttg ttattgctta ttgctatttt aaatttggag gaaaaagtag taaaggaatg
     2041 aatctaatat ttgaggtagg gcatggagaa gaagaaatta ttcctttaag attggttcca
     2101 tttataatat caggtacatg gttaacccat ttatttggtg gtagtgcagg acgagaagga
     2161 gttgctgttc agattggtgc aactttttca cattgggtag gtaaaaggtt gcctataaaa
     2221 aatgcttcta gtatttttct agtaactggt atggcagctg gttttgcagg attatttgag
     2281 acaccaatag cagcaatact atttgctatg gaggttttgg ttgctggttc actggagtat
     2341 caatcgcttt ttcctgcgtt tactgcttct tttacagcaa gtgcagtatc aaaagcttta
     2401 gggttagaga aattttcttt tgcactttct tctaaagttg tatttgactt gtcaatattt
     2461 tggaagctta ttgttttagg aattattttt ggaatggttg gtggagcatt tgcatggtgt
     2521 ctgaaattgt caaaaagaaa aatagggaat agattaaaaa atcctatgat aaggattgct
     2581 attattggag tatgtcttag tgtattattc cttttatttt ataaaggaag atattcaggt
     2641 ttagggacaa atttaattca aaatagtttc tatggtggag aaatttattc atttgactgg
     2701 ctgttaaagt ttatacttac aatactgact ttgtcagcag gttttcaagg aggagaggtt
     2761 acacctttgt tttccattgg agctagtctt ggtgtattgt tagcaggatt ttttaatttg
     2821 ccaattgaat tagttgcagc gcttggctat gctagtgtct ttggtagtgc tacaaatact
     2881 ttttttgcac ctgtatttat aggggcagaa gtttttgggt acagctattt accttatttc
     2941 tttgtagtat gcgctatttc atatatattt aatatggata aatctattta ttcactacaa
     3001 aaaatttcaa caaaacaata aataaaacaa cataatgtat gattagaaga gaaagaatat
     3061 tagagaaagg atagggtagg aatatgttga caagtttagc tttgattttt ttattaggaa
     3121 tggcctcggg aggtattttt aagaggataa aattacctaa cttattggga atgttactta
     3181 cgggaattat attaggtcca tatgttttaa atttgattga taattcaata ctagatattt
     3241 cttctgacct tagaaagatt gcattgatta ttatattaac aagggctgga ttatctttag
     3301 atataaacga tttgaagaaa gtgggaagac ccgctgtact tatgtgcttt attccagcta
     3361 cttttgaaat tatcggaatg atagttttag caccaaagtt actaggcgtt tctatattgg
     3421 aagcagcagt catgggtgca gtagtaggag cagtttctcc cgcaattatt gtacctaaaa
     3481 tgcttaaatt aatggaagaa ggttatggta cagaaaaaag tattccacag atgcttttag
     3541 caggaacttc tatagacgat attttcgtaa ttgtgatgtt tacagtattc acaggattgg
     3601 cacaaggaaa tagtatatca gctattagct ttttacaaat tccagtatca attatattag
     3661 gtgtaatagt tggagcagtg attggaatct atttagcagt attttttaag aaggttcata
     3721 tgagagattc tgctaaaggg gtgttgcttt taagtatctc atttttaatg ataagtttag
     3781 agactgcttt agagggtatt gtgccatttt ctggattatt agcagttatg agtattggga
     3841 ttttccttca gataaaatat cgtgtagttg caagaagatt gtctataaag tattcgaaac
     3901 tatgggttgg tgctgaaatt ctcttgtttg ttttggtagg ggcaacagta gatataagtt
     3961 atgcatttaa agcaggaatt ggagctgtaa ttttaatatt tggagttctt ttattccgta
     4021 tggtaggagt attcttctgc cttattaaga caaatttgac aataaaggaa agattgttct
     4081 gtatgattgg ctatattcca aaggctacag tacaggcggc aataggaggt gtaccacttg
     4141 caatggggat ggcatctgga caacttattt tgacgttagc agttctagct atacttataa
     4201 ctgcacctct tggagctttt ggaattgatg taacttataa aaaattatta acttctgtaa
     4261 aaggcgagaa taagtaaaaa taaagattat tttaactggt aagttatatt ttggaaaagg
     4321 attatactat tgaatacaat ataaaaaaat catttgggag attaagaagg tagaagttaa
     4381 gaacagattt taaggtatgt tatgattatt agaattgatt ttgtgaaatt attattgtaa
     4441 tatttaatta tatagataaa gaagcggagt gataactatg tgtttaaaaa aattatcaaa
     4501 attagagtta gttattatga aatttatttg gaatttagat ataaaaacca attcatatga
     4561 gattattgat tatatgaaag aagaacataa tttaccagaa aaagtagctt taaaaacttt
     4621 atcaaaacta tcaaagaaaa gattcttata tgttcaagaa acaggcaagt gtatgtatta
     4681 tacagttgca atcaaagaaa aggcatattt agagtttata tcaagaaatg tgcaaaatct
     4741 tttgaagaat aattttataa ggaatttatt agtatcattt cacgaagagg agctaacaga
     4801 ggaaaagata aagtcgttag aaaactgggt agtaaattgg gaagaagctt atgtataaat
     4861 gttattattt ttattacaat ttagataatt ttggttttgt aagctctgca actattttta
     4921 gatggttgca gagtttattt ttatttttta ttcccttaag caatacttaa aagtaaacgc
     4981 atttatattt actaacttca attacttcta atacttaaat tgctttttaa aaatattttg
     5041 atatgtcatc tctatagtaa tctagaactt gtttctattc tttatataaa gtttttatga
     5101 atgatatata taatattcgt tcatttgatt aaaaataaca aaatattaaa taattctact
     5161 ctgatagttt gttaaaaaaa taataaaaaa tattaataaa caaaaaaaaa tatcttaaga
     5221 gaggagaatg ttctaaaata taaaaaggtt tctagatttc ataaaagata ctattttagt
     5281 cttgaaaata tttagtttaa aaagatttta atttaatgat tgattaagtt aaaaatgtgt
     5341 atgtaataaa ttttatttta tt
//
LOCUS       DBGJFU010000187         5349 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.191, whole genome shotgun sequence.
ACCESSION   DBGJFU010000187 DBGJFU010000000
VERSION     DBGJFU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.191"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(204..1388)
                     /locus_tag="ACOIAS_003536"
     CDS             complement(204..1388)
                     /locus_tag="ACOIAS_003536"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGZ2703204.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="ACOIAS_003537"
     CDS             1761..4112
                     /locus_tag="ACOIAS_003537"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HGZ2703205.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="ACOIAS_003538"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="ACOIAS_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGZ2703206.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAKEAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKQVFLVEQYMKDDLSIAE"
BASE COUNT         2093 a          596 c          850 g         1810 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttat tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttatcattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttaaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaagcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagacg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgatatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatat ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac ccaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aaaagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaac
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tattgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagatatgt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagca
//
LOCUS       DBGJFU010000188         5325 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.192, whole genome shotgun sequence.
ACCESSION   DBGJFU010000188 DBGJFU010000000
VERSION     DBGJFU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.192"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            287..1219
                     /locus_tag="ACOIAS_003539"
     CDS             287..1219
                     /locus_tag="ACOIAS_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HGZ2703207.1"
                     /translation="MGFIGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKT
                     KIYSNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSK
                     KAGALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRD
                     KDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNG
                     FTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNIT
                     IENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1194..2021
                     /gene="panB"
                     /locus_tag="ACOIAS_003540"
     CDS             1194..2021
                     /gene="panB"
                     /locus_tag="ACOIAS_003540"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGZ2703208.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            2040..2888
                     /gene="panC"
                     /locus_tag="ACOIAS_003541"
     CDS             2040..2888
                     /gene="panC"
                     /locus_tag="ACOIAS_003541"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGZ2703209.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3299..3481
                     /locus_tag="ACOIAS_003542"
     CDS             3299..3481
                     /locus_tag="ACOIAS_003542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703210.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            4313..5227
                     /locus_tag="ACOIAS_003543"
     CDS             4313..5227
                     /locus_tag="ACOIAS_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HGZ2703211.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRYYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
BASE COUNT         2113 a          635 c          751 g         1826 t
ORIGIN      
        1 catcataatt tttctaatag acttaatcac ttttaatata tatagtaaaa caaaaaactt
       61 atattttata aatatcataa tttttttact gatattttat tatataaatt ttatagaatt
      121 aattgtcaat tttttatgaa aatggtaaga tatttatgga tggtatcttg ttataagaac
      181 tatactttaa atgtttttaa ttattttgtc ttatgtaact catatcctca ggaatgagat
      241 tagaaattaa gatgttgtat cacaaagtgg aggtgatact attaaaatag gctttattgg
      301 ttctggaaaa gttggaacat ctttggcaaa ttatttttta tcaaaaggtt attgtgtttc
      361 aggcttttat ggaaggaatc aattatcttt attagaatca acaaatttaa caaaaacaaa
      421 gatttacagc aatttgaaag atataattta tgaaaacgat atattattta ttacaacatc
      481 agatgattct attgaaatta tagacaaaaa actttccaaa tttaatttaa aacataaata
      541 catttgccac acaagtggct ctttaaaatc tagtatattg ttctgttcaa aaaaagcagg
      601 tgcattaatt tattctatcc atcctatatt tgcattttcc aataaaaata ccaacataaa
      661 aaaaatgaaa gacatttgtt tttccataga aggtgataat gaacaagatg atttagttat
      721 tcaaaatttc atagatgatt tgggaaacca attttttata agagataagg atacttcatc
      781 cacttatcat ttagctaatg ttttagtttc aaatttagta ttatctttac tagatatagg
      841 agtaagttac tttattaaat taggtttatc tgaagaggaa tctttaaaag ctatcagtcc
      901 tttagtaaaa aaaaatatag aaaatatact tgataatggc tttactaaag ctcttactgg
      961 tcctgtttct cgtggagata taacaccaat aagaaaacat ctatctgtat tggataaaaa
     1021 agatgaggat atttacaaaa tactatcctt aaacctacta aaaataattg ccttaggaaa
     1081 tgacaattca ttaaaaggta taaaaaatat tactatagaa aatgcaatcg aaaatttaat
     1141 aagtaaatct gaaaaataca aagaaattta caaactttta ggaggaaaaa acgatgaaaa
     1201 ataccataca gacttttaag aatgctaaat atgaaggaaa aaaactatct atgcttactg
     1261 cttatgacta ctcaattgca aaaataatgg atgagtgtga tataaatggt attttaatag
     1321 gagattcatt aggaatggtt ataaaaggtg aagaaaacac tctatctgtt accatagatg
     1381 aaattatata tcatacaaaa gctgtaaaaa atggtgtaaa aaatgcctta atcgtaagtg
     1441 atatgccttt tctatcttac catgtttcta tagaagatgc agttaaaaac gcgggtagac
     1501 ttataaaaga aggaggagct catgctgtaa agttagaagg tggctcaaac gttataaagc
     1561 aaatagaaag cattgtaaat gctcaaatac ctgtaatggg gcatttagga ttaactcctc
     1621 aatcagtaaa ttcatttggt gggtttaagg ttcaaggaaa taccagtgaa acagcaagac
     1681 aacttattga agatgctaaa ttaatagaaa aagcaggtgc attctctatt gtccttgaag
     1741 gagtgcctac gaaaatagct gagatggtaa ctaattctat ttcaattcct acaataggta
     1801 taggggcagg aattaactgt gatggacaaa ttttagttta tcaagatatg ttgggaatgt
     1861 ttggagattt tgttcctaag tttgtaaagc agtatgcaaa tataggagat ataatgaaag
     1921 attcaattaa aaattacatc ctagaagtta atacaggtac atttccacaa gaaaaacaca
     1981 gcttttcaat aaatgaatct gagttagaaa aattatacta atattaagga tggtacaaaa
     2041 tgcttgtaaa agaaattaaa ttactaagaa atataatcaa agattggaga aaacatggct
     2101 attctatagg gcttgttaca acaatgggat ttttacatga aggacaccaa agtcttataa
     2161 aaaaagctgt aaaagaaaat gataaagtag tagtgagtgt atttgtaaat ccaactcaat
     2221 ttggaccaaa tgaagatttt aatagctatc caagagatat agataaagat tttaaatatt
     2281 gtatggatag tggagcaaca gtagtcttta acccatcacc agaagaaatg tatttaaaag
     2341 gtaattgtac taccattaat gtttctggac ttacagattt tctttgtgga gctaaaagac
     2401 ctgttcattt tggaggtgtc tgcctagttg tttcaaagtt tctaaatata gtaacacctg
     2461 ataaagctta ttttggagag aaggatgccc aacaattagc tgtaattaaa agaatggtca
     2521 aggatttaaa catagataca gaaataatag gctgtcctat aattcgtgaa aatgatggat
     2581 tagcaaaaag ttcgagaaat acatatctct ctgaggaaga acgaaaatct gctctaatat
     2641 taaataagag cttaagttta gcaaaagaag aattagtaaa aggcaatcta aatccagaga
     2701 atataaaaga attaataaca gctaaaataa attctgaaca cttagcaaaa atagactatg
     2761 ttgaaattgt agatagtgag acattacaac ctgttaagca aatagaacat tctatacttg
     2821 tggcaattgc tgtgtttata ggaaaaacca gactaattga taattttaca tttgagttaa
     2881 atatatagtc tataatttta tataatatat ctaaaaaata atagaatacc taaaaagtaa
     2941 taaattacct aaaaaataat aaattgctta gaaaataata gattacaata actaaaattt
     3001 taaaattaaa ttccttaaaa gtatgattaa ttataattat ataagtatat tcttattaca
     3061 cttatattaa tttatattaa atatctacta actcaaatat tcataattaa actttaaact
     3121 ggataaattt tcttttaaga atgaaagttt atccagtttt gcgattataa taaccagttt
     3181 tacatatcca cttgtaaaat atatctaagc aatatatcat actatataga tttatgagtg
     3241 aaataatatc tgctatttac tgaatatttt tatttttcgt tttttagggg gattagttgt
     3301 gtttaaagat aagattgatg aatgtgttca tataatgact gcatatattg ctagtttaaa
     3361 agaatactat tcattcatag aaactcaaat tggtgatttt attaaaaaat atggagaaga
     3421 tgttgtagag ttatgtctcc atagagtgat gattctacta tgcgaatgtg gtttggcata
     3481 aaattaaatt tattaaaatg ggtaagaaga atcctatata atattcttct tacccatttt
     3541 aatatatcaa tttcatagct tatcgtttaa aatattttac atccattact agacaacttt
     3601 acattctctt tttaattcta gtatatttaa atcattttag ttgctcctta tttattctta
     3661 ttacttgttt tattttataa ataaaataat tctttttaat aatgtttttt gttgatattc
     3721 ctatcacaca tccaattatt tatcattgtc ttgaaattgt ttgcatactt aatttaatcc
     3781 aattgaaaca ccccagcata gaatcccctg taaaatagaa gttggattaa atcctaacat
     3841 ctatacagaa aatactactc caagcacaag caatataatg gaatatattt atcacttaat
     3901 tttttatact ttttaaatcc catacctaaa gcattaagtg cagctattag aattaataat
     3961 tgttgtggta taaaattatg cttgcttcat atttattcat tttttactat aaaagttctt
     4021 gttttacaaa aaacatttgt tttgtatcaa aataatactt gtaaattaca taattctcat
     4081 aaaaatttct agtattactt gtactcttaa taaataatag ccaacaagta ccttaaatca
     4141 ttatatctat atctaataag aaacacagta agataatttt ttcgcccata acttaataaa
     4201 aactgttgat attagtagca attgaattta gttaatttat ttataatata cataaattta
     4261 aatattacga ataaagtatt ataaatagga tactttctaa agggaggaaa ttatgataga
     4321 taatcaaaaa tatgttattt tatcactaga attacattta tttttttcaa gaattatgaa
     4381 agagcatgct ctttttttag aagcaggatt cacaaataaa aattataatc ttgctatgga
     4441 agctgaccac tataaaaagc aatttgaaga tttattatca tacactgtta gtgctagtaa
     4501 tggtataatt agacctgata tattatattc agaagagctt gtaactactc tcacatcagt
     4561 tgcagaacaa aaaacagaag agtttacagg gatagaaata aacaaaaaca tcactacaag
     4621 agaattaaat ctacaaagtg gtgtaaaccc acaagttggt caagacttag tgaactatgt
     4681 agctcaactt aactctgatg caataagatt acttgatggg cttattaatt ttaaagaaag
     4741 agtcttagat ggtgtactat catgtactat atttacatca aactaccctc tacttcttga
     4801 acatataata catgaagcaa atttatatcg ttattatgta gttgaccttg aaaataaaat
     4861 agatattgag tcaaaaaacg ctaaagaaat agaattattc tgggaccata ttatgatgga
     4921 acatgctctg tttatgagag gattactaga cccctcagaa ggtgaactaa taaatacttc
     4981 aaatgatttt gctataaaat ttaatgaatt aattgaaaaa acaaacgaaa tgactgattc
     5041 taatatcaag aacattacag aagaaactct aaatgaaact gttgagttta aagattttaa
     5101 agaagcagga gcatcaggaa tagaacagtg taagataaaa tctataatat taccactttt
     5161 agcagaccat gttttaagag aggcaaatca ttatattaga atattggaga gttataaaaa
     5221 catgtaatta aaaattactt ttaaaataat ctttgcatat ttagataaaa taaaggtaca
     5281 gttaactgta cctttatttt ttataaactt ataaaattta aacaa
//
LOCUS       DBGJFU010000189         5262 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.193, whole genome shotgun sequence.
ACCESSION   DBGJFU010000189 DBGJFU010000000
VERSION     DBGJFU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.193"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..483
                     /locus_tag="ACOIAS_003544"
     CDS             <1..483
                     /locus_tag="ACOIAS_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HGZ2703212.1"
                     /translation="LAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLS
                     TKLIKENDIICIEDLQVKNMIKNHKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGK
                     FFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK
                     "
     gene            610..1779
                     /locus_tag="ACOIAS_003545"
     CDS             610..1779
                     /locus_tag="ACOIAS_003545"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HGZ2703213.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1942..2484)
                     /locus_tag="ACOIAS_003546"
     CDS             complement(1942..2484)
                     /locus_tag="ACOIAS_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HGZ2703214.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2632..2916)
                     /locus_tag="ACOIAS_003547"
     CDS             complement(2632..2916)
                     /locus_tag="ACOIAS_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGZ2703215.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2979..3743)
                     /locus_tag="ACOIAS_003548"
     CDS             complement(2979..3743)
                     /locus_tag="ACOIAS_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGZ2703216.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(4025..4291)
                     /locus_tag="ACOIAS_003549"
     CDS             complement(4025..4291)
                     /locus_tag="ACOIAS_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HGZ2703217.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(4333..5142)
                     /locus_tag="ACOIAS_003550"
     CDS             complement(4333..5142)
                     /locus_tag="ACOIAS_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2703218.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIEELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         1848 a          710 c          743 g         1961 t
ORIGIN      
        1 cttgctaaat tacaaagaga attatcgaga aaaacaatcg gtagtttaaa tagaaataaa
       61 gcaagattaa aagttgcaag acttcaagaa catatagcta atcaaagaaa ggattttcta
      121 caaaaactat ctactaaatt aataaaagag aatgatatca tttgcataga agatttacaa
      181 gtaaagaata tgattaaaaa tcataaacta tctcgcttaa tttcagatgt atcatggtca
      241 gaatttattc gtcaattaga atacaaggct aattggtatg gtagacaaat tgtaaaggta
      301 ggtaaattct ttgcaagttc acaaatatgc aataaatgtg ggtataaaaa cgaggaagtt
      361 aagaacttaa atataagaga atggatttgt cctagttgta atgaaactca cgatagagat
      421 ataaatgcaa gtataaatat attaaaagaa ggattaagac taataacaat tcaaaataaa
      481 taacgtatgt aagaaccgga ggaactacgg ggatagcctg tggagaatta gtaagacata
      541 ttttagtatg caaaattctg tgaagcagga accctgtgac tttagtcatg ggaggttcag
      601 ggtagaaaga taaatgaact ttgtaaagat gaagttgtag agttttgcaa tagaataaaa
      661 ggtaaagtgg aaagtgttgc aataagtgga gtatttgctc cagtaaataa acaacaagaa
      721 gaagaagttg caaaatgggt tagagaaatt ttaggggata ttcctatatc tttatcaaat
      781 gaaataggta atataggtat attagaaaga gaaaatggag ctattttaaa ttctgcattg
      841 tctcaagtag gtaaagctgt atctttagga tttgaaaaag cacttaaaga cttaggtata
      901 gatgctcaaa tatatttcag tcaaaatgat ggaacattaa tgaatttaga gtatacaatg
      961 aaatacccaa tttttacaat tggatgtgga ataacaaatt ctataagagg agcttccttc
     1021 ttatcaaaag taaaaaatgc aatagtatta gatgttggtg gaactacaag cgacttgggt
     1081 gtactatata atggattccc tagagaatca tctattccag ttacaattgg aggagtacat
     1141 acaaacttta gaatgccaga tgttttaact attggtcttg ctggtggtac atgtgtaaaa
     1201 ggcgaaaaag ataatataaa gataggtcct gaaagtgtgg gatatagaat aactgaagat
     1261 gcaataatat ttggtggaga cactctaact ttatcagatg ttgttacaaa attagatatg
     1321 gcgtttgaag atagaaaaag caatgttgaa aacttagata aagagtattg taatgaggta
     1381 tataagaagg ttattttaaa attagaagat gctgtagacc aaatgaagac tagtcaagat
     1441 gatgttgaat tggttttaac tggtggagga agtataattg ttcctgaaaa actaaaaggc
     1501 ataagcaatg ttataagacc tcctcattat actgctgcaa atgctattgg tgcagcttta
     1561 ggacaaataa gtggagatgt agaaaaagta tacagtttag ataaaatgtc gagagaagat
     1621 gccatagaag atgcaaaaaa agaggctgta aataaagctc taaaagctgg tgctaaagaa
     1681 gatactatag aagtattaaa tatagaagat gttccacttg cataccttcc tggtaatgca
     1741 cttcttataa aagtaaaagt tgttggtgat ttaatttaaa gaataaatat aagtatttcc
     1801 agaattatat aaatatttct ggactcattt ttaaacttat tttatcaata aaaacagcat
     1861 aacttttgac tatattctta taagtttccg aacagccgat atatacctat tttttgaggt
     1921 ttatcggctg tttgttatct tttatcgcat tccaactatt tttcttaccg tatttgctgt
     1981 acgaattgta atcttatcac ttacatttga actggcagtc tttgaccacc aatttgtctt
     2041 tttataatct ttacgactaa aagaccaaaa gatagcattt ttataatgat atactttttc
     2101 atactcttct ttaggatttc caacctcatc ataaaactcg tcataagata agtcaggaaa
     2161 cataaaaata agattatcat atatttcttt gttgttatct ccccaccaat taggagcatt
     2221 atttagtata tcttttaact cttcttgtga aataataaaa actggtattt ccaattcaaa
     2281 ctgttttttt atcattaatc ttattgtatt tgagataacc ttgctgtcat ctacatcact
     2341 taaaaagaca acattaccac tattaaggta tgtagaaact tctgaataac caagttctac
     2401 aaagcttgct tttaattcag acataattat cttgttctta ccacttatat tgatacctct
     2461 taataatgcg atataacttt tcatataata atttcacctc taaacattca catttttcta
     2521 tgtagtacaa actggaattt gcatctttta taattctttc aaaacttctt catatgtaat
     2581 ttttttaata taaaaaaatc ggtgcttaat ttgtaccgat ttttgttttt cttagtaaag
     2641 aactcgtttt tttgcttctg ctatagctct agcagcactt tttgcttgca ttcttttttc
     2701 ttcttctttt ttaaattcat ttattgtttc atttagattc ttatcatgtt catttttttc
     2761 tttatcttcg ttttctctgt atactaatat ataatctatt attgattcgt ttattatttg
     2821 agatatactt ttattttcat taattcctat tctcttaact tccttaagta gagtctcatc
     2881 aatatgaacc gttgtttgct ttgtattttt tgccacctta atcacctctt aaaaaaaatt
     2941 ctaacattaa aatttttttt agtcaataaa ttttaatatt aaaattcttt aaatgtttta
     3001 tttgattttc taaaattttc tattgtattt atcatcttat tttttatatt ttcacctagt
     3061 acagaagact ttagtctagg attttcgata tggtcataaa ttggttctaa aaattcgact
     3121 tcgacaggaa cagagttaat tttaccaata ggttggtatc cttcataagt atcttttgaa
     3181 ttttttatta ctagtggaac aatagggcat tttgctttat atgctatctt taaagctcca
     3241 cttctaaact ccgaaaccaa tgaatttggt tctttaatcc atgttaaatc tccttctgga
     3301 aacactgcca tactttgacc tgtaagtata ttttgagagg cttgggctat actctttata
     3361 ccctcacgat tgttttttct gtttacatat acacatctta gtaattttgc ccattcttta
     3421 aatataggta tatttctcca aactggttca tctgcaatta cacatccaat tggtctttct
     3481 acacttgctg ttagtatgaa gctgtcaagc atactagaat gatttacaac aaatagaagt
     3541 ggttcttttg gcagtgtttc cttaccaatt atattaagct ctatgttcac tatatctaaa
     3601 gattttttag cttgtttttg cataaattca aatttttcct ttaagctaaa tttatcagga
     3661 ttctttttaa ttttaataag cctaggtata gaagatgtaa aacctataag ttgatatgta
     3721 gcaaatttta agtaattatt cataaaatta tctccttaaa ataaaattat ttattactgt
     3781 aaagatttta acataaaata aaatttaagt ctattttaat tcaattttgt aacatatatt
     3841 ttaaatagac agagattgag attttatatt tccaatttta gtaatcagat tttatcaata
     3901 aattactatt ctattaattt aattaaataa cctttaaatt taattataaa aactgttgca
     3961 gaaatttcta caacagttta aaaaatttac cttacaaaaa cttattttat aacaagagag
     4021 tatattataa ggctagtaca gcccattttt tgcactgaga aattcttttg ccatcatcac
     4081 cttgttcttt gtcatatgca aattgattaa gcatttcaca tggaaatgta tcacatagac
     4141 cacaatgtgt aaggttttta tcttcacaac aagattttac aggacattgt tcaccccaaa
     4201 atggtttctc aatctctgta caacctttac aattgacttt ttccttgtat ccacattcat
     4261 aacataaaat tccacatctt gattctatca ttttaagtca ctccttaaat ataaatttta
     4321 tatgattgta tatcaaaatt tatacttttc attgtaaaaa tcggacaaat tttttatata
     4381 ggttgttgga ttaacaccac aaatagattt aaaatcattt ataaaatgag attggtcgta
     4441 ataaccaata tcttgtgcta aagttgcaaa tggtatttta ttattttgta tttcttgtaa
     4501 tacaaggttt atacgaagta atctaaggta tgatttaaca ctcataccaa gagaattatt
     4561 aaaaattcta tttaaatgtc gttcactata acaactaatt tgtgaaatgt ttttaacaga
     4621 cataatagag ttttgttttc tagcattttc aagaattggt atagtcatat ctactatatt
     4681 tgatttcaat aagtatgata agaataacat atctaattgt tctactaatt cttctatagt
     4741 acttgttctt tcaaaaatgg aattaacctc taagctaaat aatgtgttaa aatcttcaag
     4801 tgaaattttt aaatctgtag attctctttg agaaagacct gtcaaataat atactccacc
     4861 tgggcgaaac tcaacgaata ctctaaaaag tacattttct atatcatttt ttacaatagt
     4921 agtttttgtt gtggctcccc agaatgcgct ttctatacct tttttgtcaa atttaaatat
     4981 catacatcca ctagcatcag gtattaatgt aagattttct ttaatacttg tatctggaat
     5041 agatattgta tagtgtgcta tatactgtct taacaattta tgtggcaaaa tatatgtata
     5101 ctgtttgcag gtatttaaaa tgttattgat tattttattc ataagtatat cctcctgata
     5161 tataaaaaat aagtatacta atttattttt tatataataa accagttttg ttttaattga
     5221 aatattctaa taagatattt tgttaaattt agatacaata at
//
LOCUS       DBGJFU010000190         5181 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.194, whole genome shotgun sequence.
ACCESSION   DBGJFU010000190 DBGJFU010000000
VERSION     DBGJFU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.194"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            117..491
                     /locus_tag="ACOIAS_003551"
     CDS             117..491
                     /locus_tag="ACOIAS_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGZ2703219.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            488..1351
                     /locus_tag="ACOIAS_003552"
     CDS             488..1351
                     /locus_tag="ACOIAS_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703220.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1351..1989
                     /locus_tag="ACOIAS_003553"
     CDS             1351..1989
                     /locus_tag="ACOIAS_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HGZ2703221.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
     gene            2481..2894
                     /locus_tag="ACOIAS_003554"
     CDS             2481..2894
                     /locus_tag="ACOIAS_003554"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGZ2703222.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
     gene            3085..4302
                     /locus_tag="ACOIAS_003555"
     CDS             3085..4302
                     /locus_tag="ACOIAS_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HGZ2703223.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            4464..5162
                     /locus_tag="ACOIAS_003556"
     CDS             4464..5162
                     /locus_tag="ACOIAS_003556"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGZ2703224.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         1992 a          556 c          756 g         1877 t
ORIGIN      
        1 tacatttatt aaatgtataa tattggcaat tagttttttc taaaatcagt tgacatatta
       61 tatatatatt tatataatgt gtatatacta tatatacaat ttaaggaggt gtcttattga
      121 atatagttat tagtaattcg agtgggaagc caatatatga acagattact acacaaatta
      181 aaagtatgat tataagtgga gagttaccag aaggctcagc tcttccaagt atgagactat
      241 tggcaaaaga acttagaata agtgtaatta ctacaaaacg agcatatagc gatttagaac
      301 gagacggatt tattgagaca gttactggta aaggcagttt tgtatctagt aaaaacatgg
      361 attttgtcaa agaagaacaa tttcgtttaa ttgaagaata cctacaaaaa gcagttgata
      421 cagcaaaaag tagtgatatt acatatgacg aattaaaaga tattttattt ttattatata
      481 aaggagaatg ataagtatgg atgcaataac tataaaaaat ttaaataaaa cttataaaga
      541 ttttagccta caagatatat ctttttcagt accaaaaggg agtgtaatgg gatttgtagg
      601 tgaaaatggt gcaggaaaaa caactacatt aaaagcaatt ttaaatttaa tatcttatga
      661 tagtggaaat atagaaatct ttggtcttga tagtaaaaaa aatgaaaaag aaattaaaga
      721 gcaaattggg gttgtatttg agggaagcaa ctttcatgaa aatttaaaca ctgaccatgt
      781 ttcaaaaatc atgagtaaaa tctataaaaa ttggaatgac acattattta aagactactt
      841 aaaaaaattg agagtaccag acaataaatt aattaaggag ttttcaaagg ggaataagat
      901 gaaacttagt attgctgtag ccttatctca taaaccaaaa ttattaatac tagatgaggc
      961 aactagttca cttgacccta tcgttcgtga agaaatacta gatatatttt tagattttat
     1021 acaagatgaa gaacactcta ttatcttatc ttcacatatt acaagtgatt tagataagat
     1081 agcagactat attacattta tacataaagg aaaaattgta tttagtgaaa ataaggatga
     1141 attaattgat actatgggag ttttaaaatg taaacctagt gattttgata acctttcaag
     1201 agaggattat tcatattatc gtaaaagcca atttggatat gaagtacttc ttaaagataa
     1261 gcatagattt atctcaagac atccaaactg tattgttgat aatacgtcta tagaagaaat
     1321 tatgttattt tatgtgagag gtgataaata atgaaaggtc taattttaaa agatttactt
     1381 aatttgaaag gaaatataaa gtttatactt ttattcatca ttatgtttgg ctttatgtct
     1441 tctttaggag atggaaatgt taataacttt ataggtgtta ttatagtttt atgtactaca
     1501 atgattgttt caacattttc atatgatgat ttgaataaat gggatagtta tgtacttaca
     1561 atgcctatta atcgcaatga tattgtatta agtaaatatt taactatgtt aatatttagc
     1621 tttataggtg ttcttgtttc acttattgtt agtgtaacta ttggttattt taaaaataca
     1681 cttatattaa atgaaacact acttattaat gcactaatct tatcaatttc agtatgtttt
     1741 ggtagcctta ttctaccact tatttataaa tttggtacag aaagagctcg tttattaatg
     1801 attttatgct ttttagttcc aacactagct ttattagttt ttaaaagtat tttagaaaat
     1861 attagttcac ctatttctat agaaattata cttaatacac tagtatattc tcttccattt
     1921 gttgctattt tactatttgt gatttcatat tttatatcat caaaaatcta tagtaaaaaa
     1981 gaagtataaa atatactata tatgtttctg tatttaagta tttaaataaa atactatcga
     2041 gtttattttg gtagtatttt attttttaat tattaatctc tactaggtat ttattatttt
     2101 attacttata tttaataaga ctatttacta ctttctaatc ttatttatat aatttattca
     2161 ttttataaat tgaactacta atgatattat tgaacatagc aatagaataa catctaaaat
     2221 agtttttatg tattaaatta acttatttta atattcaaca atttaggttt actaaattta
     2281 ttgatgattt ctttttatat aacttaaact acatttttat ttaaaaaaat ttaatatcaa
     2341 caatataaaa ttaagaataa gtattattag tttgacatta gcataaatgc ctattagaat
     2401 aatgttaaac tatattcttt ctactgattt tattaaaaaa taccaatata caaaagatta
     2461 tacaaggaga aagttcaaat atggaattag aaattacaga aaaaatagaa aaacaagatg
     2521 aagatactat ttttcaagga ttattaaaat ataatcttgc tagacttgaa gataaaaacc
     2581 cagtggattt aggaatatat attcgagata aagcaggtaa aattgttgca ggttttatag
     2641 gtgttactca tggtaattgg ctttctataa aatacttatg ggttagtgag aagcttagat
     2701 ataaaggtac tggaagtcaa cttttatata aggctgaaaa aattgctaaa gaacgaggtt
     2761 gtaaatatgt ttttttagat acattcagtt ttcaagcacc taaattttat gagaaatttg
     2821 gataccataa agtattttca cttgaaaatt atcctctaac tggaaagaga tattatttta
     2881 gaaaaatact ataataataa aaatcatatt ttatattttt aacttaaaac ttctgctctt
     2941 cgtctacaga ctttatattt ttatatcatt attaatacta aaatagtatt ttactataca
     3001 tacaaaatta tttgaaagtt caaaaattat aagtataaaa gctaatctta gcttaaataa
     3061 aaattatatt tttaggaggt aaatatgtta ggaaaaaaat gtatggatta tctacaaaca
     3121 ttaggaaaaa taagttcaac aacaaatggt cttacaagac ttatcttgac tcaagaacat
     3181 aaaaaatcta ttgatttaat tagttcatgg atggaaggtt taaatcttga tattgaaatt
     3241 gatgacattg gaaacgttat aggtacatat aaatcaagct ttccaaatgc tcccacatta
     3301 gtagttgcat ctcatcaaga tagtgtaaag tgtggaggta tatttgatgg tatgttaggt
     3361 ataatagtgc ctctagttgg tcttgaagaa gcaaaacaca ataataggtc ttatcctttc
     3421 aatattaagc taattgcttt cgctgaagag gaaggaacta gatttgaaac ttctttaatg
     3481 ggaagtaaag tttttgctgg tacttttaaa gaggaacttc ttaaatctgt tgatgaaaat
     3541 ggaattacac ttgaagaagc tgtaacaaag tttggattta atacaaaaaa cttaacaaat
     3601 ttgcatccta gaaaagatgt agatgcatat ttagaatttc atattgagca gggtcctgtt
     3661 ttagaaaatg aatctcttcc tgctggaatt gtatcaagta ttacaggatt taaaagtttt
     3721 aagatatcag taaatggtaa gtctggacat gctggtacac ttccaatgaa tatgagactt
     3781 gatgctggat gttgtgcctg tgaatgtgta ttagctatcg aaaaagtagc taaaacgact
     3841 gctgatttgg ttgctactgt aggaaagatg aatttctatc catcttcatc aaatgtggta
     3901 cctgaaagag ctgaatttac tcttgatgta cgctcttgtt cacaagaaat tcttgataat
     3961 tctgtagaaa agatatttaa tgaaatatca catatatgtg aaaatagaaa acttaattat
     4021 acaagtgaac ttgcttttga aaatgttcct gtaccatgtt ctaataaaat aacaaaaatc
     4081 atagaaaaaa gttttataga tttaaatctt aatccttttt atatttatag tggtgctggc
     4141 catgatgctc aggaaatgga taatataact gatattggta tggtatttat aagatgcgct
     4201 gggggcgtaa gtcataatcc taatgaaagt gtttctgtag atgacttgga tactgctgta
     4261 aaaatatttt taaaaatact agataatctt gatttaaaat aattcttata taagaaagta
     4321 tattaaaata tataatctaa aaaatcactt tatatttata ttatataagt gtacaatata
     4381 atctattata aaatataatt ttatgctttt atagattttt attttataca taaatatatt
     4441 tatatataaa ggagataatt tttatgaatg aaaaattaat tggtattttg gctggaatgg
     4501 gtccaaggtc aacagcccct tttgttgatt taattattga tgagtgtcaa tctcaatatg
     4561 gagcaaaata tgatgatgaa tttccaaaga tgatgatata ttcattacct accccatttt
     4621 atattgaccg tccaataaat catgaactta tgaaagaaac tataattgat ggattacaaa
     4681 agcttgaatc aactggtgta aattttattg caatgccatg caattcagca catatatact
     4741 ttaaagaatt aaaagagtct attaatatac caatattaaa tattgtagaa gaaacagtta
     4801 aaaaattacc tactatctca caaaaagtaa ctcttttttc gactagttca acttttgagt
     4861 ctacaattta ccagaatggt ataatacata atggacatga atttatattt aaagatgaat
     4921 ggcaaataaa attaaacaat cttattcaaa atataaaaat ggataaagaa aatccacaca
     4981 atattgatat ttggaataaa cttatcgaag atgttaaaaa tgaatctata gaaaatatag
     5041 taattgcttg tactgattta aatgtagttt tagaaaaatc atatccttca ataaatattg
     5101 ttgattcatc aaaatgcttg gctgaatcag ttgtcaataa atatctaaaa ttagttaaat
     5161 aaatttttca tgcactatat t
//
LOCUS       DBGJFU010000191         5180 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.195, whole genome shotgun sequence.
ACCESSION   DBGJFU010000191 DBGJFU010000000
VERSION     DBGJFU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.195"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            207..341
                     /locus_tag="ACOIAS_003557"
     CDS             207..341
                     /locus_tag="ACOIAS_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703225.1"
                     /translation="MDLINTIDKVQQAHKPIDEIIKTIRDIVDIIKILELVYNFKSNQ
                     "
     gene            846..1607
                     /locus_tag="ACOIAS_003558"
     CDS             846..1607
                     /locus_tag="ACOIAS_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HGZ2703226.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1672..2454
                     /locus_tag="ACOIAS_003559"
     CDS             1672..2454
                     /locus_tag="ACOIAS_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HGZ2703227.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            2721..3140
                     /locus_tag="ACOIAS_003560"
     CDS             2721..3140
                     /locus_tag="ACOIAS_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HGZ2703228.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(3243..3509)
                     /locus_tag="ACOIAS_003561"
     CDS             complement(3243..3509)
                     /locus_tag="ACOIAS_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HGZ2703229.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3736..4437
                     /locus_tag="ACOIAS_003562"
     CDS             3736..4437
                     /locus_tag="ACOIAS_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HGZ2703230.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFRETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4519..5127
                     /locus_tag="ACOIAS_003563"
     CDS             4519..5127
                     /locus_tag="ACOIAS_003563"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGZ2703231.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
BASE COUNT         2140 a          476 c          901 g         1663 t
ORIGIN      
        1 aaattaagta tgtagttaaa agtgtatagc aaaaaggctg ttatattaat gttagaggtt
       61 cagggaaaat atataaagca aagtatttac agataatgta gattgcacag ctataagtta
      121 gataatagaa ttatgtaatc aagaatttac aacaaagtat aagataagga taatgctaaa
      181 ttgccataca ggtgctagat gtgtaaatgg atttaattaa tactattgat aaagtgcaac
      241 aagcacataa accaattgat gagattatta aaactataag agatatagtt gacataatta
      301 aaatattgga acttgtttat aattttaaat caaatcaata atgattaaga ttaaaataat
      361 attaaggcat caatgttata agagtacaat ataaggagta ctatatagta ctccttactt
      421 atacatatta ggcaatagga aattttttca tagttaaatt ttcaagtatt ttttaggaaa
      481 ctttataatt gagaattaag ggtatagtat gtgatgaact tactgaattt ataattaggt
      541 tatataatgg aataatcatc aatagtttta ctttagggaa aatatatgct taaggaagta
      601 tagcttacta atattattaa aatgtatatt taaaatgata aaaaaagaat tagacaagta
      661 tttagtaaat tgactatgca aaattctatc aagcaggaac cctatgactt tagtcatggg
      721 aggttcagta ggtaacttgt tgaattaagt ttatattatt taaataaact acttaattta
      781 gataatataa aaagtcagaa ttttaagaat agagctatat aatcttagct attaggagaa
      841 ttactatgat aggaatatat tttagtggaa caggaaattc aagatactgt gtagaaaaat
      901 ttttacaaga atatgatata acagcaaatt cttattctat tgaagataat gaacttttac
      961 agcacatcaa taatcatgag aatattattt ttagttatcc ggtgcaatat agtaatgtgc
     1021 caaaaatatt gaaagatttt ataataaatc attcaacttt gtggaatggt aaacgtgttt
     1081 ttattattgc aactatggct ctatttagtg gagatggctc tggtgtgtta tctagaatat
     1141 taaaaaaata cggtgctata actgttggtg gattacatgt gaagatgcca gatagtatag
     1201 gagatgaaaa ggtattaaaa cattctttag agtataatag aaagcttgta ataaaagcag
     1261 aaaagaaagt aactaaagct gctaaaaaac tgaaacatgg gtcaccacca aaagaagggt
     1321 taggtttttt gagtcatatt gtagggttat ttggacaacg attgtatttt atcaacaaaa
     1381 caaatatgta tacagataaa ctaaaaatta atactgagga atgtattgta tgtggtaaat
     1441 gtgttaattt atgtccaatg aagaatctag taattaaaaa ttgtatgatt gttgcaaatg
     1501 ggcaatgtac aatgtgttat cgttgtgtaa ataattgtgc taaaaaagca attacattgt
     1561 taggtaaaaa agttgttgaa cagagtaata ttaaaaaata tatgtaagaa atttaaagtt
     1621 ttattgaatg taaattgatg tatattttat gttagaaaga gaggttaaat aatggaatac
     1681 tacatagttg attcatttgc aactaaacta tttaaaggaa atcctgctgg agtttgtgtt
     1741 cttgacagaa gaataccttt agagttaatg cagaaaatag cagaagaaaa caatttacca
     1801 gaaacagcat ttgtagttaa aggtaaagga aattatgaat tacgatggtt tactcctaaa
     1861 gcagaaattg atttatgtgg tcatgcaact ttagcagcag cttatgtaat atcaaatttt
     1921 attgatgtta atgtaaaaag gatagatttc tttactcaaa gtggaaagct tgaagttaca
     1981 cgaaatggaa atctatatga aatgatattt ccagaaataa tgcctattga aatagagtta
     2041 tcaccacaac aagctaattt gataggctgt gtaccaagtg ctgtttattc ttcaagagat
     2101 ttaatcttat tgctaaacag tgaacaagaa gttataaatt ataaacctaa ttacgcacaa
     2161 ttgcgtaaat tgacagactg gttaggtata ataatcacag cacaaggaag taatacagat
     2221 tttgtatcaa gatatttttg tccagaatta gattcagaag accctgttac tggttcatca
     2281 cattgtaatc ttattccata ctggtctgaa aagttgggaa aacacaaaat ggtagctgct
     2341 caactttcta atcgtggtgg tataattcag tgtgaagtgt tgaaggataa tactgtgaaa
     2401 atttctggtg aagcagtctt gtttatgcag ggaacaataa agattgatat ttaaataagt
     2461 ataataacta atttattatg aaaaataatt aattttctta gtgttaacaa taataaagta
     2521 gagttaaaaa acttgatttc acttaaatca atatgataga tgttgatact aagattgaca
     2581 agcatgatta aaaaagataa tatttaatct ataaagataa aaacacagtc tggttggtag
     2641 tccagacgca gcaatagctg tcagtaacct gcctccttgg ttgtccgttc tttagaaagt
     2701 aatttaagga ggaactagca atggacaaaa ttagtggaaa attaacagtc ttatttgaag
     2761 aaccattttg ggtaggtata tttgaaagac aagatggtaa aaaatatgaa gcatgtaggg
     2821 tagtttttgg agcagaacct aaagaagtag aagtgtatga atttatactt gaaagatttt
     2881 tctcattaga ttttggtagc attaaattag aaaaaaatgt gaccaaagac aacattggat
     2941 acaaaagaat gcagagaaaa gtaaaaaagg aacaagaaaa agaaactata gggactaagg
     3001 ctcaaaatgc tcttaagctt caatatgaag aaagaaaaca ggatagaaaa aacctagcta
     3061 aaaatagaaa agaagaagaa aaagaaagac tatttaattt gaaacaagaa aagagaaaag
     3121 ctaaacataa gggtcattaa ttttaattaa atatcttttt aataattgct agaaaaaggt
     3181 gtataaaatt tttaatttta tacaccttaa tttattgaaa agaataaggt tttatatttc
     3241 aattacttta aaatataaaa atatactaat aaaaattgta aaaatagtat aaatggtata
     3301 ccaaatgtaa atttaatgtg tttagtttta tgtctaaaag aatacattcc aattatagaa
     3361 cctatactac ctccaattac tgctatagac ataagagtag cttcttttat tctccattcg
     3421 tttctaattg ctcttttctt atctatgtac attgaaaaga aagcaataga attaatcact
     3481 ataaaatata ttagaataaa atttttcaca gtcttgcctc cataaaaata tattatacat
     3541 tataaaacaa gatatttgtt ttggaaagtt aaatttgtaa ttagtacata taaaatattt
     3601 gataagaaca aatataatat taattggaaa atttaaaaaa attaaacaat tagcaaatag
     3661 ggggtatatt tataagacac aaagtgataa aatctattta gaaaataatg taaaataaca
     3721 ggaggataaa gcaaaatgaa atttatagta gaaaaagaag tttttgataa attagaaaat
     3781 gtatgctttg gtgtagtagt tgcaaaagga atagataata ctaaagaaat agaaagaata
     3841 agcaaccttt tagatataag tatagataga gtagaagatt acttcaaaga taaaaaagta
     3901 aaggaatcag aagaaattat accatataga gaggcattta gaagtctagg catgaaccca
     3961 aataaattta tgagttcgat agaagcaatg acaacaaggg ttgctaagaa taagaaactt
     4021 cctcatataa atcctattgt agatttagga aactctattt ctttaaaata tttacttcca
     4081 atgggtgctc atgatatgga ttttagaaat gatgatgtat atgttagatt ttctaaaaaa
     4141 ggggataaat ttgtaccatt tagagaaact gatgttgaat taatggaaga aggagaatta
     4201 atttattcag ttggagacat ggtaaaaact agaagatgga tatggagaca aggagaagaa
     4261 ggaaaaatta ctaatagctc aaaaaatata ttttttccta tagatggttt tactgatgct
     4321 aatttagata aggtaatgag tgcaagagaa gaattagcaa aattgttaaa ggaaatattt
     4381 aattgtgaaa ttaaagtagg ttttgtagat aaagataatc ctgaaatgga aatataataa
     4441 gttgttgaat acttgttaag atattatatt tacttaatga tgttaaaaag gtattaaaaa
     4501 agaaaggtcg tagtaaattt gaaaaaaact ataataaaga atgtaaatcc aatgattgaa
     4561 aataagttta taggactttt tgaaatagag tataaaaaca aattagatga agataaggtt
     4621 tggatggtag catcaagaaa aagtagtgag caattaaaaa atatttattt gaagaatgaa
     4681 gaagatagtg tagatgcagt tgtaatagtt gggcttcata aaagtagtaa aaaattaatt
     4741 ttaataagac aatttagagt acctattaat ggatatatat acgagttacc agcaggtctt
     4801 gttgatgaag gagagtctat agatatttcc gttgaaagag aacttagaga agaaacagga
     4861 cttactcttt tagaaattaa aaaaaataaa agtattgata aagtttattt atctcctgga
     4921 atgagtgatg aatctgttgc ctttgtttat tgtatatgtg atggtgatat aacagatgaa
     4981 tttttagaac cagatgaaga gattgaagcg cttttaattt cgcaagaaga tgcaaaagag
     5041 attttacaaa gtaatcataa gatagacaca aaagcatttt tgattttaca aatgtttgtg
     5101 agtttaggag ctaaattatt tgaatagata tatagaaaag aggttatata gaagagtaaa
     5161 ataataaagt ttattttact
//
LOCUS       DBGJFU010000192         5128 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.196, whole genome shotgun sequence.
ACCESSION   DBGJFU010000192 DBGJFU010000000
VERSION     DBGJFU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.196"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            148..1674
                     /locus_tag="ACOIAS_003564"
     CDS             148..1674
                     /locus_tag="ACOIAS_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2703232.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1702..2259
                     /locus_tag="ACOIAS_003565"
     CDS             1702..2259
                     /locus_tag="ACOIAS_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703233.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2781..3824
                     /locus_tag="ACOIAS_003566"
     CDS             2781..3824
                     /locus_tag="ACOIAS_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703234.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            3935..5077
                     /locus_tag="ACOIAS_003567"
     CDS             3935..5077
                     /locus_tag="ACOIAS_003567"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGZ2703235.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2164 a          423 c          922 g         1619 t
ORIGIN      
        1 atataagttt agtctggtgt ataaaacaag aaaactaagt gatttttaat ttaacttttt
       61 attaagctta ttttttatac tatttataat aaataaactc catgatggga aaactattac
      121 tatttaatat gtaaaggagt attttaaatg aatgaatcat attggttttt aaactcaagt
      181 ccaaaagaat ataacaaatt aggtgaaaat ataaaaacag attgtcttat agtaggtggt
      241 ggaataactg gattgacaac tgcatattta ttagcaaaag aaggtaaaaa agtagtgctt
      301 gttgaggctg ataaaatagg atatggaact tctggaagga atactggtaa ggtaacttgt
      361 cagcatgata tattttactc taagatagag aaaaaatatg gattagataa agcaaaatca
      421 tattataatg caaataatga agctttaaat ttagttgaac aaataataga agaaaacaat
      481 ataaaatgtg attttaaaag ggaaacttct tttgtattta cagaaaaaga ggatactata
      541 aaggatatta aagatgaata tcgtacatgt aaaaaaatag gaataaattg tgagtatcat
      601 gaaactatag aaaatattcc attagatata aagggggcta ttagttttac taatcaaggt
      661 caatttaacc caaagaagta tattgatgga ttggcaaaag cagcagtaaa tttagggcta
      721 aaaatatatg aaaatacacc agtagtagac cttgaaaaag gaaaaatatg tagggttaaa
      781 acaagagagg ataatataat agaagctgag aatgttataa tttcatctca tagtccatgg
      841 tatgatggat tgaattttta ttttgcaaaa gaatatgcag agcgtgctta tttaatggtt
      901 gcagttttgg aaaataaatt agcagatggt atgtttataa gtatagatga cccatcaata
      961 acttttagac aatataatga tggaagtgaa aatttactga tttttggagg aggagaccat
     1021 aaagttggtc agggaggaac tgagaaagaa atatttgatg atttagagca ttatggtaaa
     1081 gaagtcttta aggtaaaaga ttttaagggg aaatggtctg ctcaagacaa tatgagtttt
     1141 gacaatgttc cttatattgg ttatatcaac aaaagagaag acaatattta tgtagctaca
     1201 gggttctcta aatggggaat tactaatgga actgctgctg gaataataat aaaagatttg
     1261 ataataaata ataattctga ttataaagat acttttaatc catccagatt aggtagctac
     1321 ttttcaaaag attttataaa ggaaaatgca aacgtagcaa taaattatgt aagtggaaaa
     1381 ttaaaaatag gtagtggaga tatgcctaaa aataatggag aaggaaagat tgttaatatt
     1441 gatggaaaaa gatatggtgt atataaggat gataatggag atttttatat tgtagacact
     1501 acatgtactc atttaggatg tgaacttaat ttcaacagtg aagaaaaaac atgggactgt
     1561 ccatgtcatg gttctagatt tgactataag ggaaatatac ttgaaggacc agctcttaag
     1621 ccattaaaac tttatggaca tggtgataat gatgttaatc ctaagttatt ataaaattat
     1681 ttttaggaag gtaaagtgta tatgaataaa cttttaaagc caataactat actattgtat
     1741 atagtttata ttttatctgc cgtatttttt gtagcaaaca aagaatatgg aagtctaggt
     1801 ctggtagcaa ttagtttagt tggtactttc attttgagtt ttataaataa gcaaacggaa
     1861 agattattga gtaaagaatt atatataatg cttgctttat ttataatgtt ttcatcattg
     1921 ttaggtactt gttttaattt ttatgatata aattattatg atgatttttt gcatttatgg
     1981 tcaggtataa tatcttgtat tgtggctttt tctataatta aatactttta tacacaaaga
     2041 gagataagta aaatgagtaa agtgttttta gttatatttt tatttatgtt tagtatggga
     2101 gtagcgagtt tatgggaaat aggtgaattt ttaatggata cttttgttgg gactactaca
     2161 caggccggag ggttagaaga tactatgatt gatatggtag atgctttaat aggcacaatt
     2221 ataactattc cttttatagt aaaaaaatct aatatatagt tttaaatttt ttatattaat
     2281 tataatggat ttaattaatc atgttgagtt tcaaggtcta tatgattaat taaatccatt
     2341 tttgttattt ataattatta ttgaatatat ttttacatat aagatataag gtgatagata
     2401 gaaattgttg gattatgtaa ataattaata tataatacca ttatgggcaa taaatacaaa
     2461 tcaaatgata atatagtcta tttatgtaaa tgttatattg tatactaagt ataatagtaa
     2521 gttttttgat agtggtgtag aaagtaaaaa tctactagcc taaattgatg tatggattag
     2581 tttaggaaca gtaattgcat atcttaagtt tgatgtcatg ttgggctact agaaataggt
     2641 catctttttc acttgaatat cttttcttta aacatggaga gtatcatatc aaaaacatat
     2701 gaaaattaag tgatttataa gaacagtaat attttatatg atagtataga atagtaataa
     2761 agtattagga ggaacattga gtgttaaaaa agtggtttgg tattgtgaaa aaaagacaaa
     2821 aaagtgagtc agcaaaagaa gaaggtgaag taatattaaa aaacgaaaaa atattatctg
     2881 aagaaaagtt gatagatgaa gaaggagttg tagttaatat tgataatgaa atattaacta
     2941 aagtagaagt agtaaatgat gataatgaaa tagaagaaaa aataatagaa gaagattggt
     3001 taattagtga aaataccata aaattagatg ataaagaagc aattattaat gataaaaaca
     3061 tagaattatg taaaaaagaa gttcaagttg aaagtgaaaa aataggttta aataagtttg
     3121 aaggacttga ccaaaatgaa aattataatc tagaaaaaaa tgttattgaa gaaaaaaaag
     3181 taagtgaatg tttgacagaa gaagatttag agtacataaa agaaattaaa ataaaaagag
     3241 gaaaaagtat aaaagctata aacttgtata ctaaagaaga gtgggttttt gacactcata
     3301 tacagtgtag taaaaaactc aaagttccat tagggtacat aagagaaaat ttaaaatatg
     3361 gatatatgga ttactttgga gatgcaataa attatttaag tgaagtatta aatatagatg
     3421 aatactgcaa aagtgaatgg agctatctcg ataatagcaa atctccatct gagatattta
     3481 atattctaaa caataaaata tttagtataa ggctttctaa tgaaaaaaga aatgaaattt
     3541 tgacaaatga taaaattgaa gcattaaaaa tgaattatag atttgaatgt attgatgaag
     3601 aatatgatga atattttaaa aaatataagt ctataatcaa aagaggtgga aagaaaaaag
     3661 ttgaattagt aaataaaaaa ggtgacattt tagaaatatt taagtcttta gaagaatgtg
     3721 ccatttattt gcaaaaggaa aagaatgaag ttatacaaat gttaaaatat ggagatacaa
     3781 aagtaggaag aaactttata aggtatagtt tgagaagtat ttaatttcat tttaaattca
     3841 taagagtttc atttctgatt cacacattta atattatttt aatgtaaaat aataaaggtg
     3901 aagtattgca caattaaaat taaggagggt attaatgtct gaagaaaaaa atccaaaaga
     3961 aaatacatta gatgaatcta aaaattcaga taatgtaaat tctaaaaaag atgatgtgtc
     4021 agatgaaaaa aatactgata acaaagttaa aaaaagggaa ataaagctaa acaacaaaac
     4081 acttaaagct attgtagcaa ttgttgcagt aggagctata tgtttaggtg gaggctatgt
     4141 ttttggcaaa gaagctggaa gaaaattgcc tgctacttca aaacactata atagcagtga
     4201 ggtagtagct actattgggg ataaaaaaat aacaggtgaa atgttaagaa agagaatgga
     4261 gccattattt tatttaaatg caaaaactac tatgactgat gacgaaatac gttcatatga
     4321 atcaaatatg attgaaacta tgattacatc tgatatatta tcctctgaag cagcaaaaga
     4381 gaaaataagt ataactaatg accaactaga tgctcagtat aataatttaa tgagtagtat
     4441 agaatcagca atgggaatga ctaaagaaga gtttttaaaa cagtttgatt tgactgaaaa
     4501 atatataaaa gatgatatga aaaaagagct agtagcatca aaatatttaa atgaaaaatc
     4561 aaaagttact gataaagaag cagagaacta ttacaataaa aacaagagca attatttaca
     4621 agttagagct tcacatattt taataaaaac tgttgatgat aaaggtaagc aagttagtag
     4681 tagcaaaaaa gctgaactta agaaagaagc tgaagaaata cttaaaaaag cccaagctgg
     4741 ggaagatttt gctactctag ctaaaaaata ctctgaagat agtagtgcag aatcaggtgg
     4801 agatttaggt ttctttggta aaggtcagat ggtggaatct tttgaaaaag cagcctttgc
     4861 attgaagaag ggagaggtat ctaacaaact tgtagaaagt gattatggat atcatataat
     4921 taagaaaact gatgaaaaat atcagccatt tgaagaggta aaatcagatt tagtaagcag
     4981 tttaacatca gaaaaacaaa acctacttat acaaaatcta aaagaaaaat ataatgttaa
     5041 aattacagat aaatatcaaa tgaaagctaa agaatagata aagtcaattt attaaaaaaa
     5101 agaaaaatta actgtatgga taaaagct
//
LOCUS       DBGJFU010000193         5056 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.197, whole genome shotgun sequence.
ACCESSION   DBGJFU010000193 DBGJFU010000000
VERSION     DBGJFU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.197"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(1..192)
                     /locus_tag="ACOIAS_003568"
     CDS             complement(1..192)
                     /locus_tag="ACOIAS_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703236.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            463..702
                     /locus_tag="ACOIAS_003569"
     CDS             463..702
                     /locus_tag="ACOIAS_003569"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2703237.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            1011..2630
                     /locus_tag="ACOIAS_003570"
     CDS             1011..2630
                     /locus_tag="ACOIAS_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGZ2703238.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDEHFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            3004..3198
                     /locus_tag="ACOIAS_003571"
     CDS             3004..3198
                     /locus_tag="ACOIAS_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2703239.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(3757..4080)
                     /locus_tag="ACOIAS_003572"
     CDS             complement(3757..4080)
                     /locus_tag="ACOIAS_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HGZ2703240.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(4125..4379)
                     /locus_tag="ACOIAS_003573"
     CDS             complement(4125..4379)
                     /locus_tag="ACOIAS_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703241.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(4643..4924)
                     /locus_tag="ACOIAS_003574"
     CDS             complement(4643..4924)
                     /locus_tag="ACOIAS_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703242.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT         1853 a          657 c          556 g         1990 t
ORIGIN      
        1 ttatctatgt tttaaataca caaaatattc ttctacttca tctccatttt ttacttttac
       61 taaatattca ttataatgag cattatatgg ctgtgtcatt ttgcattcta ttacattttc
      121 tatgtcttct aattctaaaa gttctacatc ctttaaaaat tttctttcta tgagttccat
      181 taatctttcc ataatatatc ctcacttccc ctataattaa gataaactat atatcctctt
      241 aatgcagagt ttatctgttt attatattat atccaatata ttacagtttc taacatcatt
      301 cataaattat tttaacaaat agagattgat tttttaataa atatgccttg ataaaatgaa
      361 acatagacca actcttagtt ttatgtaaag aaaacttaaa gaaaactata aattatattc
      421 acaaaaatta ctcagaagat ataacagtaa gtaatatatg taatgaatta caacttaata
      481 aaagttattt tttctttgtt taagaaaaaa agtggatata cttttactaa ttttttaaat
      541 aaagttagag ttgaaaaaag caaaaagtat atattaaaaa aagacttgtc tattttagac
      601 gttgctgtac taattggatt caatagtcaa aattactata gtataatttt taaaaagttt
      661 aataacttta caccaattga atataaaaat atttataact aaaaaatatc attctaaaag
      721 gatgttattt ttttacatat tttttacaat tatagttaat tctttgttaa attgtttgta
      781 cataaaatac taaaaatcta agttcaagta tttaaattgc tatatatcat aaattttcta
      841 aaattaacaa caacttaaaa atttaacatt ctcttaacgt atacttaaca aaacattaat
      901 atttttattg acaaaatttg ttattctata ttatggaaac aataaatttt agttaataaa
      961 gtttataatt cagataaaaa aattattaat ttcttaaggg aggaaatatc gtgaatatag
     1021 taattagcct tatcggtgga cttggacttt ttctttacgg aatgagttta atgggagaag
     1081 gtttacaaaa atcagcagga gacaaattaa aaaaaattat tgaactctta acaagtaatg
     1141 ttgttatggg tgtactggta ggtacagtcg ttacaggtat tatacaaagt tcaagtgcta
     1201 ctactgttat ggttgtagga tttgtaaatg caggtataat gaatctaagt caagctattg
     1261 gtgtaataat gggggcaaat ataggtacta cagttactgc tcaattagtt tcatttaatc
     1321 ttgaaggaat tgcaccaatt gctttaggta taggtatttt attctattta tttacatcaa
     1381 atcaaaaaac aaagcattta tctgagatac ttataggttt tggtatatta tttacaggaa
     1441 tggagtttat gaaagatgca gtagctccac ttgctgaata caaagcattc actgatgcac
     1501 ttttatattt tagtaaaaat cctgtattag gtatattggc aggatttgcc ataactggta
     1561 ttgttcaaag ttcaagtgca tctatgggta tgttaatagc actagcttct caaggaatac
     1621 ttcctctatc atctgcttta cctattcttt atggagataa tataggtact tgtgttactt
     1681 cacttttatc aagtgttggt gcaagtagaa atgctcgacg tgcagctgta atgcatttaa
     1741 gttttaatgt aattggtaca ataatattta tgcttgtttt aaataaacct atatcagcca
     1801 tagttactca ttttgaccca actgatacag ctagacaaat agcaaatgca catactttat
     1861 ttaatcttac aaatgttata atattattac cattttctaa atacatagtt aaattagcaa
     1921 atagacttat acctattaaa gaaactgaat cagaaatagt aaataatact aagtacttag
     1981 atgaaaggat gtttagcact ccatctatag cacttggtaa tactgtacaa gaagttgtaa
     2041 gaatgggtca taaagctaca aactctttgg aacattcaat tgcaggattt ttaaataaga
     2101 gcaatgaaga tattaataaa gcttttgagt cagaaaaagt tgtcaataag ttacagaaag
     2161 atatattgaa ctatttgtta aaactttcta aagaacctct tagagatgat gaacacttta
     2221 gaacagattt gctttttaat actgtaaatg acattgaaag agtctctgac catgcagaaa
     2281 atatagctga attggccatg agtgttaagg aaatgaacat tagtttttct gatagtgcga
     2341 taagagaaat atacgaaata tacaataaga caattactaa ttttaaggat gccttaatag
     2401 ttttagatgt taaagatttt gagttagcaa ataaagtttt agaagttgaa aatgaagtaa
     2461 attatttaga aaaaactttt agaaattcac atatgattag actaaataat ggaagttgta
     2521 caattgatgc tggtgtactt tatcttgact tacttactaa tctagaaaga atttctgacc
     2581 actcaacaaa tatagtaaaa caagtactaa aacttaaaca aaaaatatag tgattatttt
     2641 tatatataat cttaatcaat gagaagtaag aagctataga taatgcttat atcatttatg
     2701 gtttcttatt ttttatgtaa tactcaaatt tttatagtat tctcttatta ttaaacaatt
     2761 tctgcctaga atattccata tatatatgac ttctttcata aaacttgaac tgcccataac
     2821 taaagtcaca agtatacata actatttttt aatcaattat aaccaatatt tgttttattt
     2881 atcttcattt taattcttca ttcaataatg taataagatt attttttatt tggatactat
     2941 tatatcatct aaatatgtta ttatatattt ggatattttc atattaatag gagggattct
     3001 acaatgataa attatatggt agaaattata tatagcgaaa aaataagcga agtagaaaac
     3061 atgttgaaaa ttgaaaatat acaatatgaa tttacttctc gtgatggctt tatatttttt
     3121 ggtttttatg atatgacaga cgcatataga tttgagactt tatgttcaga tgaaaaaatt
     3181 cgatttaaaa gaatataata gataaaaaag tacatatttc taaactattt gtttttcaaa
     3241 tttaggttgt gttttaatgt agtgttgata ttataaacaa ttttaataat aaaaaagtag
     3301 ggacattata ttaatatgct cctacttttt actcatactt attaatatta tttatcaaaa
     3361 ttaccctaca aactggaatt tatcattctt tcataatttt aaactatcat atttaaaata
     3421 tataaaattc cacatgattt ttgtataaac ataatatcaa ccattatcca atttaaaaat
     3481 attttttagt aatttccatt tcattttctc ttaacatatt aattaaattt ttatatgtaa
     3541 atggtgtatt tacatattat ttaatatcca acaattatat aaagaaatgt gctctttgcc
     3601 ttataaagaa ttataaagcc aattcgtttt attatattat ttttctttat accttaaaaa
     3661 ctttaccttc aaattcttat ttatctatat attataaaaa tgttcttaac ctcttttacg
     3721 agattttctt gacatattat cagttagata aaataattaa ataatataat cagcaagtat
     3781 gttttttact cttttaagta aatttccacc tgcaccccaa tcaattttta aaattccaat
     3841 tgcactgcct gttgaagtag ctttaataac tgtttttcct gtggtatttt cataaactac
     3901 tattaatcct gcatcactat tatttcttgc ataatacttc tcaaaagttg taattagtat
     3961 aaatttatca tctcctaaat tataagaatc actaaaaact aaatctgcat caagattttc
     4021 ttttatcatt ttttgtgcct ctaagggtgt aatttttaca gtaaatattt ctgttgccat
     4081 tttttatctc ctcataagaa tttattttag acaaaacata atctctagta ttttctgata
     4141 tatttatcaa acaatatccc tattcctcct aacagagcta atatagaacc aaaatttcta
     4201 tattgagaaa tagattggtc tagataaata ttatattggt cagtatccat gattccacca
     4261 ttttgttgaa taatatgctc tgcactaaat tcacctaaac ctatacaaca gaataatact
     4321 actactccaa ttacaaaagc aaatatacat attgttaatt taaaatcctt attttccata
     4381 gttatccctc tcataagcta aatttatttg attattttta ctagatttta tattactctt
     4441 attatacaat acaacatata tttacatcta tttccaaaat tgtaaccttt atagttttat
     4501 atatttaaat tttttaaaca aaaaatacac atctataaat tataaatgtg tattctgtac
     4561 cattactaat aatatattat acttttttac aaatttctaa ttatttataa ctattcaaaa
     4621 ataaaattca tataaaataa ttctattgta caattgaaac tttaataatg tctccatttt
     4681 ttctctccaa gtgaactact tctccagtta aatcatagtc aaatgcctta actaatattt
     4741 cagtaacttt tttaccatct actacatcat cttttccatt aaataaatcc aaggcattat
     4801 tctcaatcat tctctttgca aaatttacag gtaactttat atgtatttta tcgcctgtat
     4861 gtgataatac tcttattctt aaaaactttt ctaaattgtt tcttggtgtt tgttttgatg
     4921 ccatactatt aaatcactct cctttatttt ttatttaata ataaatttta ttcttaaaaa
     4981 tatctttata tgtatttatt atgaaagtaa cactattatt ttctaatctt gttttatatt
     5041 ccttcttctt taatat
//
LOCUS       DBGJFU010000194         5044 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.198, whole genome shotgun sequence.
ACCESSION   DBGJFU010000194 DBGJFU010000000
VERSION     DBGJFU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.198"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(64..1521)
                     /locus_tag="ACOIAS_003575"
     CDS             complement(64..1521)
                     /locus_tag="ACOIAS_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HGZ2703243.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            1939..2853
                     /gene="nadA"
                     /locus_tag="ACOIAS_003576"
     CDS             1939..2853
                     /gene="nadA"
                     /locus_tag="ACOIAS_003576"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGZ2703244.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            2866..4167
                     /locus_tag="ACOIAS_003577"
     CDS             2866..4167
                     /locus_tag="ACOIAS_003577"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGZ2703245.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            4148..4984
                     /gene="nadC"
                     /locus_tag="ACOIAS_003578"
     CDS             4148..4984
                     /gene="nadC"
                     /locus_tag="ACOIAS_003578"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGZ2703246.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT         2007 a          599 c          867 g         1571 t
ORIGIN      
        1 gagattgcct acttttttat cattttttaa taaaatttat tctaagtatt attttattta
       61 gatttagtca gccaacttag tatctgcttt gctttttaac tttctaccag agcgtacagt
      121 taaaatagca tataatacag taaatatggc agctaaaaca tatgatatat ttatgtttaa
      181 gttaaatcca atttgagcat taaatatata actgaatata acaaaggaat aaaacatacc
      241 aggaattaag cttataatat aattcttttc ttttattata aggtaaactg taatcattgc
      301 aaatgcaaat atggcaatag tttgattact ccaagagaag tatctccaca atatattaaa
      361 tccagaagca ctaagctttg caaatataag gatggctatg acaggaataa atatacagat
      421 tgacaatata acacgatgtt tcttttcctt ttggtcataa tgaaagtaat ctgcaagcat
      481 taatcttaaa gatcttaaag ctgtatctcc agaagttatt ggtaaaacaa ttacacctat
      541 tatagctatg ataccaccaa tagaaccaag taaatctctt gcaaccaaac ctattacatc
      601 aggagaacct actaattctt taggtattcc tttattgtaa atacccatag cagctgcagc
      661 ccaaatcatt gcaattaaac cttctaaaat catcatatta taaaaagtag tttttccttc
      721 tttttcattt gaaactgatc ttgctataag agtagcttga gttgagtgga atccagaaac
      781 aataccacaa gcaactgtaa caaagaatgt aggaataaga ggaataccat caggatgaat
      841 acctttccaa tttgcaagac ttaaattagc taaatcataa ccttgtgtaa agattccaat
      901 tccaacacca gcagcagata aaagtagaat agctccaaat attggatata cctttcctat
      961 gattttatca attggaaata gtgttgcaca caagtaatat agaaagataa gtccataaac
     1021 tatccagaca actggattat ttatagtcga tttcatattt aaaatttgag taacaactaa
     1081 atccccaggt gtataaataa aaactgcacc aactaaaagc attaaaagac aaaggaatat
     1141 attatagact tgaaatactt tattacctaa aaatctactg ataagagaag gcatttgtgc
     1201 tcctttttct cttatactaa tcattccact catatagtca tgcaaagctc ctccaaatac
     1261 acagccgata ggtattagta tgaatgctat tggaccaaac aatatacctt ggataggtcc
     1321 tagaattggt cctgtaccag ctatattaag caactcaatt agactatttt tccatttttt
     1381 cattgggaca tagtctactc catcactttg agcaagtgca ggtgtttttc tgtcatctgg
     1441 gccaaatact ttttcacaat aagcaccata taaagcacct cctattagta atatagccaa
     1501 gccgattaag aatgttgtca taatatcacc ccatttataa aattttttgg tctttgtata
     1561 catattatct aaatgtgtag atttatattg aatttaattc tggacaacct acaaattcta
     1621 taaagtatta caactaatat aatttcagaa ataaatacat atacataacc atgtacttag
     1681 tatacaggta tttggtaata ttttcaatgt tatagcaata aagaatataa acaaaattct
     1741 gataatattt tacaaaaaaa ttaaaaaata taaaaattag tttaaataga tggagaaaaa
     1801 aggaaaatca attgagaata taaatgtcta tatcttaaca atattaagag gtttaaaatt
     1861 cttgaagaaa gaatagtcaa gtgatataat gggtttacag gtgtatatac actagtacat
     1921 atagaaagcg gtgcaattat ggataaagat ttaacatatc aaatcaaaga gctaaagaaa
     1981 gaaaaaaatg caataatact tgcccatttt tatcaaccac cagaaataca agaattagca
     2041 gatgcagttg gtgactcata ttatttaagt gagattgcta gagattgtaa agaagaagtg
     2101 gttgtatttt gtggagtaag gtttatggga gaaagtgcta agatattatc acctgaaaaa
     2161 actgtattga tgccagtaag taacgctggg tgtgctatgg cagatatggt agatgaagaa
     2221 ggcgtgataa aattaaagca acagtatcca aatgctttgg ttgtatgtta tataaactct
     2281 acagctaaag ttaaggcaca ttgtgatgtt tctgttacat cttctagtgc aattaaaata
     2341 ctagaaaaca tagataataa agaaattata ttccttccag ataaaaattt aggaggatat
     2401 attgcagaac aatttccaga taaaaacttt atattttggg acggatattg taaataccat
     2461 aacaatataa gagctgaaga aataattgag ttaaaagata agtataaaaa tgcagaagta
     2521 ttagtacatc cagaatgtaa aaaagaaata agagatttag gagattatgt aggtagtaca
     2581 tcaggaataa taaaatatgc tactaatagt aaaaacaaag actttataat agcaactgaa
     2641 gaaggtatat tacatgaatt aaagaaaaat aatcctaata aaaactttta tattcctggt
     2701 ggaaaaatac tatgtacaga tatgaaaaag acaacattag aaaatttata tagtacactt
     2761 aaaaatatgg aaaatgaagt tattgtagaa gatgaaatca tggaaaaggc tttaaattca
     2821 ctgcttaaca tgcacaaatt agcagaagga tagagggata gacttatgaa tttagaacaa
     2881 gatgttttaa tagttggaag tggagtttca ggattatact gtgcattaaa tttagataaa
     2941 agtttaaatg tgctagtagt atcgaaatca acaattgaaa acaacaatac atatcttgca
     3001 caaggtggaa tttcaactgc tagaaatatt gatgatatag aatcctttgt agaagataca
     3061 atgaaggctg ggcagtataa aaatagagtt gaagcagttc aagtacttgc agatgaatct
     3121 atagaaaatg taggtcagat agtagaatat ggtatgcctt tagacaaaga aaatggagag
     3181 atagattaca caagagaagg agcacatagt gtaaatcgaa tagttcatag taaggataat
     3241 actggagaag ttgtttttaa aactttatta aaagaagcta aaactagaga aaatataact
     3301 cttatagaag atgcatatct tttagatata ttaaaagatg gcaataaatg tataggagct
     3361 agaattttta aatctaaaaa agagattcat gttttttcaa aaatagtagt tttagctaca
     3421 ggtggaattg gtgggctttt taaaaattct actaatcaaa gacatcttac aggtgatgga
     3481 attgcaatag ctttaagaaa taatataaaa atagaaaatt tagaatatat tcaaatacat
     3541 cctacagcct tttatgaaga aaataatgaa ggtaggagaa tgcttatatc tgaatcatta
     3601 agaggagagg gagctaaact tttaaataaa aataaagaaa gatttgtgga tgaacttctt
     3661 cctagagatg tagtttcaaa agctattttt gagcaaatgg aaaaagataa attaccttat
     3721 gtgtatttag atgcaacaca tttagatagt gaatatttaa ttaatagatt ttcttttatt
     3781 tataatgaat gtttagctag aggaacagac ataacgaaag aatgtataaa ggtatctcct
     3841 gcacaacatt attttatggg cgggatacat gtagatttgg attcaaaaac atctatggaa
     3901 aatttatatg cagttggtga aattagttgt acaggtgttc atggagccaa tagattggca
     3961 agtaattcat tacttgaagg gcttgtattt tcaaaaagag cagctaaaaa tataaactca
     4021 gttatagata atgtaaaagt taagtttata gatgtaccag atatggatat agatatagaa
     4081 caggtaaaaa aagaaaataa aatattagtc ataaaagaaa ttgaaagaac aagtgaggat
     4141 tttggcgatg aattatttga ttattgatag aatgatacag gatgctttga tagaagatgt
     4201 tccaagtgaa gatataacaa ctaattctat tgtggatgaa aattctaaat ctactgtaga
     4261 tttaatttgc aaacaagatg gtataatagc tggcttaggt gtatttaaga gagtttttga
     4321 gatacttggt gatgtagatg taaaattata taaaaatgat ggtgatagag taaagaatcg
     4381 tgaaaagata gctgtattaa caggtagtac tagaaactta cttgtaggag agcgtgtagc
     4441 attgaattat ttacagagaa tgagtggtat agctactatt acaagtaggt atgttgaaaa
     4501 gcttgaaggt acaaatacaa aattattaga tagtagaaaa acgataccta atcttagaat
     4561 attagataag tattctgtca aagtaggtgg aggatgcaat catagattta atttatctga
     4621 tggaatattg cttaaagaca accatattgg ggctgctggt agtgttaaga aggcagttga
     4681 attagctaga aaaaatactt cttttgttag aaagatagaa gtggaagtgg aaactttaga
     4741 catggtaaaa gaagcaatag aagccaatgc agatataata atgttggata atatgagttt
     4801 ggaaatggca aaacaagcag tagatattat aaatggaaga gccattgtag aattttccgg
     4861 aaatgtaaat ttagatacta tagaagatat aggaaaaatt ggtgtggatg ttgtttctgt
     4921 aggagcgctt acacattctg ttaaagctct cgatataagt atgaagaatt tgagaaatat
     4981 ataaatttta tattgaaata aacttaatta aaatagactc cttaaatttg ttttataaat
     5041 ttaa
//
LOCUS       DBGJFU010000195         5008 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.199, whole genome shotgun sequence.
ACCESSION   DBGJFU010000195 DBGJFU010000000
VERSION     DBGJFU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.199"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            25..828
                     /locus_tag="ACOIAS_003579"
     CDS             25..828
                     /locus_tag="ACOIAS_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703247.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            840..1970
                     /locus_tag="ACOIAS_003580"
     CDS             840..1970
                     /locus_tag="ACOIAS_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2703248.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1974..3383
                     /locus_tag="ACOIAS_003581"
     CDS             1974..3383
                     /locus_tag="ACOIAS_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HGZ2703249.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3600..4103
                     /locus_tag="ACOIAS_003582"
     CDS             3600..4103
                     /locus_tag="ACOIAS_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HGZ2703250.1"
                     /translation="MFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYS
                     NNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGK
                     CEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVSSY
                     ISSHIPQ"
BASE COUNT         2086 a          422 c          861 g         1639 t
ORIGIN      
        1 taattataga aagaaaggca cattatggaa aaattgagag ttattgaaat gataaaaaat
       61 aatcccaata ttatagctac aatagataat ccaactgatg aaatgaaatt gttagctatt
      121 aaagaaaatg ggcttgtact agaatatata aataatccaa ccagagagat gcaagaacta
      181 gcaatagata ataatattag agcaattaaa tttatagata atccaacaga agatatgatg
      241 ataaaagcag taaatgaggg atggagtata ctggattata tcaaaaaccc aactgataaa
      301 gttattgaaa tggctataaa tcaagcagga tgggcaatta aatatgctaa aaatccaagt
      361 gaagaattac aactgttagc agtaagaaaa aattatgatt cgataaagtt tataaaacaa
      421 ccttatgaaa gtgtacaaga ggaggcagtg agaataagtt atgatgcctt gagatatata
      481 gattcaccta gttataatgc agaacttata gctataaaaa ataatgaagc agctattagc
      541 tttataactg atttagataa aaataaaatg ttggaatttt taaaagtgaa tattttagtg
      601 attaagtaca ttctgaaaaa agtatctaaa gatgaattag agagtgtatt aaaagaagta
      661 ttatccaaag aagatgttga ggaaaagtat attagagatt ttttgaattg tagtgttata
      721 gatagaaata gcgaaagtat accaatagat aaaattatgt ttatttataa atatggaagt
      781 aagacagcaa aaaaaatagc tgtagatgag aaattaaaga ttatgtagga gagagaaaaa
      841 tgaattttat agacacatta aaaagcgttg acttaatatt atccactgga gaagtacctt
      901 taatagtagg tgaaagtgga ataggaaaaa cagctttagc aaataagctt gccaaagaaa
      961 atgattggag tttaattgtt attgatggaa acttacttaa agagggtgaa ataggtggtc
     1021 ttccaactat agaatcttat gtaggagtta attctaatgg atataagact gaaaagaaaa
     1081 caacagtgta tgctgttcat aacaaattaa gagaaattga tgaagagata tctaaaagta
     1141 agacagttct tttatttata gatgagataa atcgttgtga gcatacagta cagcaagaac
     1201 ttatgaattt aattttaaat agagaaatta atggatataa actgcatgat gatgtaaaga
     1261 ttttagcagc aatgaatcca tcaagtaaat atggttcaga ttttgattat caagtggtag
     1321 atatggatgc agcccaagaa aataggtttg tttggttaaa tatggagagt gaccatacac
     1381 aatggataaa atgggcaata gatgagggta ttgaaagaaa agttatcgag tttatttcca
     1441 cttttccaga atatctgcat aaaataaatg aagatgatgt aagagccact cctagaagtt
     1501 atgagagagt atctaaaatt tataaagttt ataaagaaaa aaataattct attcctagag
     1561 cagtattttt aaatgttgta aagggtaatg taggtaaagt tattgcagaa gaatttataa
     1621 gttttattga atcaaactct gaaccattaa tatcctatga agatgttttt ttaggagaat
     1681 ctattgatga gtctattgta gaaagagtaa aaaatgagag tcatacaaga ctttatctat
     1741 cagctatgaa cattctaaaa gacttagaat taaatattaa gaatgataaa tatgaatcaa
     1801 accactatat taatagattt attgaatttt taaaaatgta tcctgtagat ttaatgatag
     1861 gtattatgaa ggatattaga aacagttata ttgaagtata taaaaaagct atagaaaatg
     1921 aagagtttgt aaaatcatat tttgaatctt atagtttaat aaggggataa tgtatggaaa
     1981 attattttga taaacaagca aaagagcttt ataataaagc aagtgaaatt ataaatactc
     2041 attctatgct aaaggcaaat cgtgctaatg agaaatttga tatagatata ccacaagatt
     2101 ttaagtatga gttttttagt cttgtagata aagtgaattt aagtcttatg gaagaagaag
     2161 ataattttta tggatatttt ttgtttcaga tgtcaagaga aataagattt gatataagta
     2221 gtcctacagg tgtgaatttt aagggagcta aatatgttat atattttaat ccaataatct
     2281 ttttaacgct tgacataaag caaatggaga ctacaattaa acatgaaatt catcatatat
     2341 tatctatgca tttgatgaga gcaaaggagt taaaaggaaa gtatagtaca ttagcaatta
     2401 atatggcaat ggatatagta gtaaataaat ttctaaacaa tttaccacca tatgcaacta
     2461 ccttagagtg ggtaaattta aagtattcct taaaacttga accatatgaa ccttttgaat
     2521 attatgtaga aaagattcag actgaattag atttgatgga agtggatgaa gatggtgagg
     2581 aagatgatag tgataaatat ggagatatag aaattgatta tagccccgaa agaactcatg
     2641 atatatggga tgaatccaat gagatagatg aaaataccct tagagaattt actgaaaaat
     2701 ttattaacag ttctcaaaaa ggtaaagttc ctgcttattt agaaagtatg atatcatcac
     2761 ttaaaaatag taagggtgaa ttgccatgga atttatatct taatagaata atgggaacag
     2821 ttgaaaataa taaaaagaag actataacaa gaagaaatag aagacaacca aatcgtttag
     2881 atttgagggg agaacttaga agtcataagt cagaaatagc agttgcactt gatgtaagtg
     2941 gaagtattag tgatgaagag tttaaacaag ctattaaaga agttcttagt atagtgaaaa
     3001 actataatca tgaaattaca attatagaat gtgataatga aattagacgt gtatataaag
     3061 tgagaactga aaaagatata aaagatagaa ttaggatagg tggaggtact agattttctc
     3121 cagtctttga gtatgctaat aataaaaaga ttaatttgtt agtctacttt actgatggta
     3181 aaggtgaaaa taaactacaa gtaataccta gagggtataa aattttatgg gttatttctg
     3241 gaagaggaga taaaatttca ttaaaagaac cttatggagc agttaaaaaa cttactaagg
     3301 ttaaaacaaa tgatgaaata ttagaaataa atgatgtaag aaatgatggg tactcaatga
     3361 atagccaagc accaatttta taggtattca caggagataa gctgtattaa tttattttga
     3421 tatgcttgtt ataaaaatct aaaaaataca ttaatcaatg gttaatgtat tttttaattt
     3481 taataatata agttttactt acatttaagt cttaaaatgt gttaaaatat aatatatagc
     3541 agatagtttt ggcaaggtgg atttagttgt caaaactatt ttttttatta aggatggtga
     3601 tgtttttgtt taagattgat gattatataa tgtatggtat gacaggagtc tgcaaagtca
     3661 tggatataac aaatgaacga tttacaaatg gaataaaaaa agagtattat gtattgagtc
     3721 ctatttattc taataataca gtaataaaaa ttcctgtaga taatgaaaaa gtaccaatga
     3781 gaaagttact ttctaaaatt aatgtgcttt cattaataaa tgatattcct aatatggata
     3841 catcatggat agataatgaa aaattgagaa gtgagcaatt taaaaaaata ctcagaggtg
     3901 gtaaatgtga agagttaata aaattagtaa gaagtataga taataacaga gagtatgtaa
     3961 aatctattgg taaaaaaaca catcaagcag atgataatat aatgaaagaa gctgaaaggt
     4021 tattaaatga agaatttgca actattttag atatttctcc taatgaagtt agttcatata
     4081 tttcaagtca tatacctcaa taaagtgttt tagtatcctt tatttaatct attttaagta
     4141 ttatttatgt atgctggttt tggtaaaata aaatatataa ttaaattata taaaaaataa
     4201 tgatgattgt attgaagaat tacagaatag tgcggagagc ctactccttt aggtgtgaga
     4261 tggatatcac ttaaagattt aaattgttat tgtagatgtt tttaatataa aacaagatta
     4321 ataagtgtat tgtaagcttt aagtgaaatt gatactataa tgagttactt agattttaaa
     4381 atatataata ttataaaaaa tccttttagg ttttgaatga ataagatgga tactatgttg
     4441 aatagaatcc atcttattta agttttattt atgttgttta tttgtaagca ttatttttgt
     4501 acatgaaaac acagtgtaaa aatatgctta gaattatgaa gttttattaa aaagatattc
     4561 gacaaaataa gactaaaaat attcttaaac ttataactaa gataaagatt gtatatttat
     4621 agtgtagcct gaaaaattgt ggatgtacga aatttattta gtattttatt gaaacaaagt
     4681 agaagaagta ctatagttta tctttgaata gattgttatt ttttagtgaa aaagttatta
     4741 ggttaataca taaatataat agcttataaa tagtttaagc atgttaaaaa agtagttatt
     4801 agagatatat agttacattg cttataagat acttaaaatg atagcattca cttatattta
     4861 gaaaattaag ataaaaaatc atttttatat agtaaagcat acctaaattt agaaaataat
     4921 tgaatttata aaattaatta aatacataaa aaagtattca atcaacttaa caaatttttt
     4981 taaatttaat ttaaattttt tattctat
//
LOCUS       DBGJFU010000196         4975 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.200, whole genome shotgun sequence.
ACCESSION   DBGJFU010000196 DBGJFU010000000
VERSION     DBGJFU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.200"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            120..1748
                     /gene="aspD"
                     /locus_tag="ACOIAS_003583"
     CDS             120..1748
                     /gene="aspD"
                     /locus_tag="ACOIAS_003583"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047688 - aspartate 4-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HGZ2703251.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1869..2864
                     /locus_tag="ACOIAS_003584"
     CDS             1869..2864
                     /locus_tag="ACOIAS_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGZ2703252.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3053..3337)
                     /locus_tag="ACOIAS_003585"
     CDS             complement(3053..3337)
                     /locus_tag="ACOIAS_003585"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2703253.1"
                     /translation="MEKTYAWKHTTTITLLARLTKKGFFISQRIGKHVHYRVLIKEKE
                     YLKIEGKRIFGGLHNNPLSELISKLHDGEEITEEKIIEIGNWIKSWKDED"
     regulatory      3662..3759
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3901..4258
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3901..3929
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4544..4702)
                     /locus_tag="ACOIAS_003586"
     CDS             complement(4544..4702)
                     /locus_tag="ACOIAS_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703254.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
BASE COUNT         1776 a          681 c          720 g         1798 t
ORIGIN      
        1 attttaattt catactttac tttttctata aacctattat aattaaagta taatcatttt
       61 tgcaacttgt ttattttatt ataaaaatgt gaaacttatt acataaggag gaatttaaca
      121 tgaaaaatca tacttataaa gaaatcaaaa acatatatgg aaaaataagc ccttttgaat
      181 ttaaggacaa attaatagat attgcaaaat acactgcaaa agaaaataac agagaattac
      241 ttgatgcagg tcgtggaaat ccaaactgga cttgttcaac tgctagagaa gcctttttta
      301 cttttggtca tttcgctatt actgaaactc gttctaattg ggatttaggt catttggctg
      361 gaatgcctca aaaaaagggt attaaagaaa gattttttaa atttataaat gaaaatatag
      421 atatgcctgg agcatattta gctagagata ttataaactt tggtataaat gaacttggat
      481 ttgatggaga tgaatttgtt catgaactag ctgatggaat tattggtgat aactatccac
      541 ttccagatag aatgttacct catatggaaa aaatagtaca cgattaccta gttcaagaaa
      601 tgaaatatga tatatcaggt aaatatggtg atgtagaaat ttttgctgtt gaaggtgcta
      661 cagctgctat gtgctatata tttgattcat taatggcaaa tgaacttcta aaaaaaggag
      721 ataccatagc cttaatgact ccaatattta caccttatct tgaaatacct aaccttcctc
      781 gttacgactt taaagtggta aatataaatg ctaatgaagt tgatgaaaaa ggtgctcata
      841 catggcaata tacaaaaaaa gaattagaga aacttcgtga caaatctatt aaagctttat
      901 tcgttgttaa tcctaataat cctgcttcta tagctatgga tgaaacatct tgtaataatc
      961 taatagatgt aattgaaaat tataataaag atttaatgat aatatcagat gatgtatatg
     1021 gaacttttgt agaagatttt tcatccttaa tgtcaaaact accttacaac actgttggag
     1081 tatattctta ttcaaaatat tttggagtta caggatggag acttggtaca tttgcccttc
     1141 ataagaaaaa tgtttttgat aagaaaataa atgatttaac tggagaatta aaaaaatcag
     1201 tagataagcg ttacagtgat atgagtctta acccttctag tctttccttt atggaaagag
     1261 ttgttgctga tagcagactt gttgctctca accatactgc tggtctttct actccacagc
     1321 aagtacaaat ggcgtttttc tctgcttttg cattgattga caaagtagat gcctacaaaa
     1381 aacttaatat gagtatctgc catagacgtc aaaaactatt atttgaagct ttagagcttc
     1441 caattaatga aaacaaaaac aatgctgcat attacactca atttgatatt gaagaatggg
     1501 ctaaattaaa ctacggagaa gacttattta aatttataag taaaactgca tctccagtag
     1561 atgttcttta taaattagct aatgactatt cagtagtttt acttagtgga aatggattct
     1621 atggccctga atggtcaatt agaatatctc tagcaaatct atatgatgaa gcttatacta
     1681 agataggaaa agccataaga gagatattaa atggttatat cgttgaatgg caaaaaatta
     1741 agaaataatt atattacact gaaatatcta aattatatga ataaaagagt ttatgtagac
     1801 cttaactact ttttttgata taatcctatc ggaattataa attttattta tgaaaggcag
     1861 agaatagtat gagtattaag aaaaaagttg ccatagtttt tactggtgga actatatcta
     1921 tgacagtaga tgataaagtc ggagcagcta taccaacttt atcaggtgaa caaatactat
     1981 caatggttac caacattgat aaagttgcag atgttgaagt ttttaatttt gatgaaattc
     2041 caggacctca cataacacct tatagaatga tggaattgaa aaattatgta aatgatttat
     2101 tagcaagaaa agatattaca ggtgttgtaa taacacatgg tacagatagt ttagaggaaa
     2161 cagcttattt tctagacctt acaattgaca acatcaaacc tgtaatagtc acaggtgcta
     2221 tgagaagtag ttctgaactt ggatatgatg gttcaagtaa tttatctgca tcagtttgta
     2281 ctgcagtatc caaagatgct atgggaaaag gagtccttgt agttttaaac aatgaagtat
     2341 tattagcttc tgaagctact aaaacaaata ctctttctct aaatacattt aaatctctaa
     2401 ctagtggccc attaggaata attgactgca acgaacttgt ccttacaaga gatattgtaa
     2461 atagaacaat tatagatact gataaggtag aatctaaagt tgccttattt aaagcatatg
     2521 taggagaaaa tgctgacttt ataagatttg ctgttgatag tggttataaa ggtatagtta
     2581 ttgaggcaat gggcagaggt aatattcctc ctcaaatgct ttctggagtt gaatatgcta
     2641 gagaaaaagg acttcctgta gttatagttt ctagatgtca ttcaggtaga gtattcgata
     2701 gttatggtta ctttggttct ggtagagatt taaaaaatct tggttgtata tttggtgggg
     2761 acttaccagg tcaaaaagct agaataaaac ttatcttagc acttggaaaa actgataacc
     2821 ttgatgaaat caaagacttc tttgaaaaag gaatctattg ttaattctaa taacttctat
     2881 ttaattattt attgatattt caacattttt aaatcaaaat ctaatactta tgtcagttaa
     2941 atgtcagcaa aatattttat ccacaatttt tacacaaatt ttccacaact tatccccaat
     3001 attatccaca agaaaaaagg tagcacttat attagtagct accttttatt tattaatctt
     3061 catctttcca gctttttatc caatttccta tttctattat tttttcttca gttatttctt
     3121 ctccatcatg taattttgaa attagttctg ataatggatt gttatgtaga cctccaaaaa
     3181 ttctttttcc ctctattttg agatattctt tctcttttat taatactcta tagtgtacat
     3241 gtttcccaat tctctgcgat atgaaaaaac cttttttagt taatctagct aaaagtgtaa
     3301 ttgtagttgt atgcttccaa gcatatgttt tttctattta atctgcaaca tcttttgata
     3361 ttacagtttt ataaccagta ttccatatgt atttcataat ttttaattct gcatctggta
     3421 attttttatt caacataata tattccttct ttcaatttat gtcacttcct caaacttatc
     3481 tagcttaaat taataatagg agctttttat cacaaaaact acaggtaaat ttttctatat
     3541 attctacgta tttagtctgt ttttaagtgt gaaattacgt tcatacctat aaatttattc
     3601 aattttcaaa caatcttgtt gaataataac taattctatt gtattattta gttatagaat
     3661 aaaactaaat cggcaaaact agagaaatct agtgacgcaa agctataggg actaagactt
     3721 atataaatat cttatgagtt atgttagcca gttgccaaag agatattgtt ctttttgttt
     3781 ttatgaaagt tttttagggg gataataatt tatatgttta gagataaaat ggataaatgt
     3841 acacatatat tgactgctta tattagcaat tcgtatgatt attgtaattt tttggataca
     3901 atttacattc catatagtta atataaaact caattaaatt atgaccttgg tttagatagt
     3961 tcattattta cattccatat agttaatata aaaccagaag gaatatctgg agttattgga
     4021 gggtctattg gatttacatt ccatatagtt aatataaaac atttactaag tgtagatata
     4081 attataaata cgatttaatt tacattccat atagttaata taaaacctgt agacttcaac
     4141 cctacaatgc ttgttggaag tgtatttaca ttccatatag ttaatataaa acttttgtaa
     4201 aagtttagaa gtttatatat aaaaaaaata tttacattcc atatagttaa tataaaaccc
     4261 caaaataaat tgagtatttc caatgcctac acatatacaa atctctcaaa tttgcagtga
     4321 accatgagta gtgcaattga taacacttat cacaagtcct caatgcctta tatttcaact
     4381 gttaagcctt aatttcttca aaaaatcgaa cactgcaaaa tttctatgac tttattatat
     4441 cataaaaata ttatttttga atatctgtac caatttgtga tataataaaa gcaagaagaa
     4501 ctacaatcta ttttgctgta gagtggagtt cataatttag aaattactta gttttatgga
     4561 atctgatttt taaatcaaat tcccaaccac tcttagttgc cgctttgagt ggttttttac
     4621 gctttctaaa tagtttgcta gctatataaa ctattaagct agcagataga cttgctagta
     4681 tgccttgtaa aaaattatcc atgtatattc acctcccttc ttagcgtcgg gaggataatt
     4741 ttttgataca tgaactccac tctataaatt gtagattaca tcttcttgct aaaaatatta
     4801 taacatataa ttcttacata ttttacctat ttattacttt ttacattata ataatattct
     4861 tttaatcaaa aaaacaccta ctattaaagt aaatgttttt aagtattttt aattttaaaa
     4921 tccatatagt tcatatgaaa ctaaatataa taactcaaca tatgagcaat atcga
//
LOCUS       DBGJFU010000197         4884 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.201, whole genome shotgun sequence.
ACCESSION   DBGJFU010000197 DBGJFU010000000
VERSION     DBGJFU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.201"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            483..1343
                     /locus_tag="ACOIAS_003587"
     CDS             483..1343
                     /locus_tag="ACOIAS_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGZ2703255.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1333..3204
                     /locus_tag="ACOIAS_003588"
     CDS             1333..3204
                     /locus_tag="ACOIAS_003588"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGZ2703256.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3224..4678
                     /locus_tag="ACOIAS_003589"
     CDS             3224..4678
                     /locus_tag="ACOIAS_003589"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGZ2703257.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKIGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1878 a          514 c          894 g         1598 t
ORIGIN      
        1 aatgaaacta aatttgaagc tttacctatt aatatatctt aatagtgatt aaataaaact
       61 aaacttaaga ctttatatgt taatatatct taagaattta tttgttaaca tacagttcat
      121 tatatttaaa agcattacta tgataattca taatagtaaa atagataatg gttcagacaa
      181 tattataaat attagtgacg gtaaaatagt atcaatttag tatataaacc ttgtcagata
      241 atcgcataac agagcttgta gttatagaat aaaaaaatat ctaggaaacc gttgacagat
      301 ttatatggat agtataacat atagaatatg aaattaaaca aaaaagaatt ttaattatgg
      361 cgacgtagtc ataattttga gtatgttact gattagcagg cagaactcaa gtatatatgt
      421 gaaaaataga gggatactct atacatatat gcttgagttc tttttattga aaggaggaat
      481 atatgaaaat aattcagatt ttaaataata atgtagcctt agtaaaaaga ggtaaaaatg
      541 aaattattgt atttgttaaa ggaattggct ttaaaaaaag agtgggacaa gtaataaatg
      601 aaaatgaaat agagagaaca tatatactag attcatacga tatgctagaa cactttagtt
      661 atctactaca aaactcagac tccaataata tagttcttat taatgaaata attgaatatg
      721 gtgagaaagc tttaaatata aaggcaagtg attatctaag cttaacctta ctagaccatt
      781 tagagttttt acttaaaaga tgtgaaaaga atcaatttat aaaaagtcct ttggtatgga
      841 atgttaaaag attttatcca aagcattttg aaattggaat gcatgcacta aaattaattg
      901 gtgatagtaa gggaattaat cttcctgatg atgaggcagt atcaatagca cttcattttg
      961 taaatatgga ggtaaataaa gagtctcatg atagtacaat tgtagagttg agaactttag
     1021 cagatatagt ttctataata aaatatcatt ttaatgtaga gctagatgaa acttcaacaa
     1081 attatatgag atttactact catctacagt actttatcca aagaattatg tcggatgaag
     1141 cttcaataga tgaagataat tctatagatt tatataaaca agtaagtcaa ctttatccaa
     1201 agtcatttgg aagtgttcaa aagataagaa aatatataaa atcacaattt aatgtcgata
     1261 tatctataag tgaagaaact tatttaatgt tgcatataaa tagggtgact caaagactgg
     1321 aggcaaaatc agatgaaata taaagattta aatgaaaata taataaaact tataggggga
     1381 aaagaaaata taaagtcggt agcgcattgt gttactcgtt taagatttac tttaaatgat
     1441 aggagtatcg ctcaaacaga agaaattaaa aaattaaaag gagtaattga tgtagtttct
     1501 aatgatgttg cttatcaaat tataataggt actcatgttg tagatgtata taacgagttt
     1561 atgtctatga taggtttaag ttcaagtgaa tcaagtataa aaagtgaaag aaaaaaggga
     1621 attagaggta tcaaaggtat atttaattcc atctttgtgg tagtctcaga gactatgact
     1681 tcagtaattg aagtactact tgcagctggt atacttgcaa gtatattagc acttttaaat
     1741 atgtctggca tacttcagag tgacagtcct acatatcaaa tattagatac attaagaagt
     1801 gctacatttc acttcttacc agtattaatt gcaatagcat cagcaaaaag acttaatgta
     1861 aatccctata tagcagtagt gttagcagtt acgttattat cttcatcaat agatggtgta
     1921 gaaggattaa gtttatttgg tataaattta caggctacaa cttatgcaaa cagttttatt
     1981 ccaatacttt taggagtatg ggcaatggga attataattg aaggtttgaa aaaaatatta
     2041 cctaaagcat tacactattt tttagttcca gtgttatctt taataataac tctatcagtt
     2101 acattaactg tatttggacc tattggtatg tggattggtg atggattaaa ctatgcatgt
     2161 atgtttttaa ttgatacatt gggtaattgg agtgttgttg cactgtatgc agctttacaa
     2221 ccactcttaa tagtttttgg tgcagcaaac tttactatgc ctatatcttt aaattctgtg
     2281 acaacattgg ggtatgaccc aatttttatg ggcgctgcaa caatatctga tattgcagtt
     2341 tgtggtgcaa tgtttggata tttcttaaaa actaggaata aagaacaaaa acaactattt
     2401 ggtacagtaa gttttagtgc acttatggga gttacagaac cttcagtgtt tggtgtgttt
     2461 atgaaatata gaagaccatt tgtagcagta atgataggtg gaggtatagg tggtttaata
     2521 gcaggtttag ctcaagttaa aacatatgga atggtttggg gattagctgc attaccaacg
     2581 tatcttgttg gaggagaggt atctaacttt acatttatga taataagtgt agtagtatca
     2641 tttgtagcag caacagcagc ttcctattta ctaggaatac cttcagatga aaaagaagaa
     2701 gttaagggtg ataatgaaaa agaattagaa ctaataatca acgagaacaa ttcaagaact
     2761 attagtattg gaaatgttgc aaaaggtcag gtaattgcac ttggggaagt taatgacaaa
     2821 gcattttcct ctggagcatt aggtaaaggg gttggagtta tacctagtga atcaaaatca
     2881 actgttatat ctcctgtaga tggaactata actatagtat ttccaactaa acatgcatat
     2941 ggtatagtga ctgatgaagg attagaaata ttaattcata ttgggataga tactgtaaac
     3001 ttagatggaa aattctttga aagtcttgta acacaaaatc aaagggttaa aaaaggagat
     3061 gctttagcag tatttgaatc tgataaaatt atagaggaag gctttgattc tactataata
     3121 actgtagtta caaatactag cgattattta gatgtaatct catcaaatga tgaatcagaa
     3181 gaattattaa ctgtaatagt ataaatttta ggagggaaaa ttcatgaata ttaaagatac
     3241 atttttctgg ggaggaagta ttgcagccca tcaatgtgaa ggttcatggg atagtgataa
     3301 caaaggacct gcaattatgg actttgtaac taagggctct tatgaaacgc caagagtaat
     3361 tactgataaa atagaagaaa aattagatta tccatctcat aatggaatag atttttataa
     3421 tagatataaa gaagatatag ctttatttaa agaaatggga tttagtgcat taagaatatc
     3481 tatagattgg tcaagaattt ttccaaatgg agatgatgaa aatcctaatg aattagggat
     3541 taaatactat gaaggtctga tagatacatt aatagaaaat aatatagaac ctattgtaac
     3601 actatatcat tttgaattac ctatgaattt agtacataaa tatggttcat ggaataacag
     3661 aaaacttata gatttgtatt taaagtacag tgagactgta ataagaagat ttgatgataa
     3721 ggtaaagtac tgggttacat ttaatgagat gaaccatatt gaccctcaaa cagagcactc
     3781 ggatattttt acttatatga tagcaggtat taagtatagt gaaatagaaa ataaggttca
     3841 aacattggca aatgtagggt ataatatgac tttggcgggt gtaaaggttg caaagttggc
     3901 tagagaaatc aatcccaaca ataaaattgg atgtgtattt ggactaaatc caatttatcc
     3961 atacaattgt aatcctagta atgtgttaaa tgcattttta gataatgacc gtgattatta
     4021 tcaaattgat gcaatgtgca atgggaagtt tccacaatat aaattaaaag aatatgaaaa
     4081 aaataatata actattgaag tgactgatga agacaaaaaa gcatttaaag aaggtaagtt
     4141 agattttata ggacttaatt attatttttc aagtgtatct actccaaaag aaaatttatc
     4201 aggtgataaa tctttatttg gtgggattca aaatccatat ttggaacaaa gtaagtgggg
     4261 gtgggcaata gaccctatag gtttaagatt taccttgaac tatatttaca gaaagtatca
     4321 attacctatc ttaattacag aaaatggttt aggggcatat gataaaatcg aagaagatgg
     4381 aactataaat gatgattata gaattgaata tttaagtaag catctatctc aaatgaagtc
     4441 tgcaattgaa gaagataatg ttgaatgttt tggatatttg atgtggggac caattgattt
     4501 agtcagtgca actacaggtc agatgagtaa gagatatgga tttatctatg ttgacttaga
     4561 tgatgaaggc aaaggaagtt ttaagagata taagaaaaaa tcatttgatt ggtacaaaga
     4621 agtaataaaa tctaatggag aagttttaga tttttctacg gatatagtag tagggtagga
     4681 ataagataaa tagaagttag gaactaatgt taattagtgt ctagtaaagc atttaaaata
     4741 gagtataggg tctttaaagt aaagtgtgta ttcttcttaa aaataatggc aattaaaatt
     4801 actataaaaa ggagaataca cattttttat ttttatactt aagtaaatat ttagacatat
     4861 tcatagtata atttactcat tata
//
LOCUS       DBGJFU010000198         4822 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.202, whole genome shotgun sequence.
ACCESSION   DBGJFU010000198 DBGJFU010000000
VERSION     DBGJFU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.202"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            120..911
                     /locus_tag="ACOIAS_003590"
     CDS             120..911
                     /locus_tag="ACOIAS_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HGZ2703258.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            940..2160
                     /locus_tag="ACOIAS_003591"
     CDS             940..2160
                     /locus_tag="ACOIAS_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HGZ2703259.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2180..2875
                     /gene="pxpB"
                     /locus_tag="ACOIAS_003592"
     CDS             2180..2875
                     /gene="pxpB"
                     /locus_tag="ACOIAS_003592"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGZ2703260.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            2905..3891
                     /locus_tag="ACOIAS_003593"
     CDS             2905..3891
                     /locus_tag="ACOIAS_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HGZ2703261.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4086..4652)
                     /locus_tag="ACOIAS_003594"
     CDS             complement(4086..4652)
                     /locus_tag="ACOIAS_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGZ2703262.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
BASE COUNT         1816 a          564 c          900 g         1542 t
ORIGIN      
        1 gttgcaaaaa taaaattgaa tacaagtgat aaaatatttc atgcaatata taaaataaac
       61 aaaatttaaa aattaaaact acttattcta tcaaaatgtt agcaaggagg ggtcataaat
      121 tgtataaagt agatttaaat agtgatttag gagaaagttt tggaacgtat aagattggtt
      181 tagatgaaga agttttaaaa tatatttcat cagctaatat agcttgtggg tttcatgcag
      241 gtgaccctag tcatatggaa aaaacagtac agctagctaa aaaaaatgga gtgaaaatag
      301 gcgcacatcc aggttttcta gatttaatag gatttggaag acgtgagatg aaaataacaa
      361 agcaggaagc taaagattat acaaaatatc aattgggtgc tttaatggca tttgcaagtt
      421 caaatggatg taatatacaa catgtaaaac cacatggagc attatacaat atggcagcta
      481 aagataaaga gttagctatg gggatttgtg aagctatata tgaagtcgat aaagatatta
      541 tactgttggg gctatataat agtgaaatga taaattctgc taaagaaatt ggacttaggt
      601 ttgcaaatga agtatttgca gatagagctt atgataataa tggttttttg gttccaagaa
      661 atgtagaagg agcagttata catgatacaa aacatgcaat agatagggtt gttaggatgg
      721 taaaagaagg aactgtagaa actttaacag gtgaagttat acatatcaaa gcagattcta
      781 tatgtgtaca tggggacaat ccaaaagcaa tagagtttgt caaagaaata agaaagcgtt
      841 ttgaattaga aagtatagaa gtttgtccat tagaaaacat agaagtttgt tcgtcagaga
      901 acatagtata agactgataa catgaggggg agataaaata tgagtacaaa aaatattaca
      961 gataaaacta aaaataaaaa agatgtcgga gcacttatag gggctgcatt tataatggca
     1021 acatcagcaa ttggaccagg gtttttaact cagactgcac aatttaccca agattttgga
     1081 ccgaattttt catttgtaat tttaattaca acaatccttt ttataggggc tcaagtaaat
     1141 gtatggagag taattggtgt atcaggactt agaggtcagg atatagcaaa taaaataata
     1201 ccaggattag gttatttagt agctttttta gtagctcttg gaggattagc ttttaatata
     1261 ggaaatgttg gtggagctgc acttggtatg aatgtaatgt ttaatatgaa tatgacatta
     1321 ggaactgtat taagtggatt aatagccata tttgtattta tgtcaaaaaa ttcaaattct
     1381 ctagtcgata agataactaa atttttagca ttaggaatga taattatagt tggatatgta
     1441 gcaatatcaa atcatcctcc tgttggagag gcagtatcaa gaatggtaaa accagaaaat
     1501 ccaaaaggac tgatattccc aataataact ctacttggag gaagtgttgg aggttatata
     1561 acttttgctg gaggacatag attaatagac ggtggaataa caggagaaga aaatataaaa
     1621 gaaataacta aatcttctct tttgggtata ttagttgcca caatgatgag agttctttta
     1681 tttttagcaa tactggcagt ggtttcaaaa ggtcttcaac ttgacccaga aaatccagct
     1741 gcttcagctt ttaaatttag tgcaggagca ataggatata aattctttgg acttgtatta
     1801 tggtctgctg caattacatc agttatagga gctgcttata catcagtttc tttcttaaag
     1861 acactaaatc cttttataga taaatatgaa aaatatttta taatagcatt tatagcaata
     1921 tcaacactta taatggcatt tataggaaaa ccagctactt tattaatttt agcaggagct
     1981 ttaaatggat tgatacttcc aatcacatta ggaattatgc ttatagcatc taaaagaaaa
     2041 gatattgttg gagattacaa gcatcctaca tggcttttaa tatttggatt aatagtagtt
     2101 ttaatatctg catatacagg aataacttct cttagcagcc taggtgcttt atttgcttaa
     2161 tttagaagag gtgacattta tggaaactag atacttgata tcaggggata aggcagtggt
     2221 tgctgaattt ggaaatgaga tatcagaaga tataaacaaa aaagtaattt catttatgag
     2281 agcaatagaa atatcgaatc ttaaaggtat tgtaacagaa atggtaccaa catataggtc
     2341 acttatgata agttataatc cattagaaat agattttgat agtttgattg agaatttaaa
     2401 aaaaatagaa gataatttgg aaagtatagt attaccaaaa cctaaaatac atgagattcc
     2461 agtatgttat gatgaggttt ttggtataga tattaaaaac gttgcaagct acaataatct
     2521 cactgtagat gaagttataa aaatacacac aagcagagag tacttgatat atatgctagg
     2581 ttttactcct ggatttccat atctaggtgg tatggatgaa agaatagcta cacctagatt
     2641 agaagtgcca aggactaaaa tttatggtgg gagtgttgga atagcaggtt cccagactgg
     2701 tgtatatcct attgatagtc ctggtggatg gcaaataatt ggtaggacac ctttaaaatt
     2761 atatgatgaa aatagagaag aacagatttt gcttagagct ggagatttta taaaatttgt
     2821 tccaataaca ttagatgaat ttatagaaat cgaaaaaaat aacttaagta tttaaatatc
     2881 gttatagtac agggagtggt atatatggga ttcaaagttg aattaggagg tttccaaaca
     2941 ttaatacaag acagaggtag agttggctat ggtcaatatg gtgtatctgg atgtggagca
     3001 atggatgagt atgcacatag agtaggaaat atattagtag gaaattctga agatgaggct
     3061 tctttagaag ttttaatgtt aggtcctaca attacctttg atgaatatac gcaaatagca
     3121 gtaactgggg gagatttagg ggctaaaata aatggaaaag aaattcaaaa ctggaggtca
     3181 tatcaaataa atccaggtga tgtattatca tttagaggtg tgaaatcagg tgctagagcc
     3241 tatgtgtcaa tagctggtgg aatagatgtt ccacttgcaa tgggaagtaa gtcaacttat
     3301 acaagagcta aaattggtgg atttgaaggt agagcactta aaaaaggaga ttatataaat
     3361 acttttattc aggaaaaaga ttttacaata aataaaaaat taagttcaaa atatattcca
     3421 acttatagtt cagaaatcgt actaagaatt gtaaaaggtc ctcaatttga tgctttttct
     3481 aacggtgaag tagagaaatt tttatcaaat aaatacaaag ttacgaatga aatagataga
     3541 atgggttgta gactagatgg agagagtata aagcacctta atggagcaga tattatatct
     3601 gatggtattt cttatggagc tatacaagta cctggtcatg gaaaaccgat tataatgtta
     3661 tcagatagac agactagtgg tggctatact aagataggga acgttataag tgtagatttg
     3721 tataagttag cacaagcaaa gccaaatgat gtagtaaaat ttgaacttgt agatatatat
     3781 gaagcacata gactattgag agagcaagaa gataagattc aagatatcta caagagtatg
     3841 aaaaatataa gagttgtaaa ggcaaaagta ttaaatgata tagcagttta attttaacaa
     3901 atacaatttt tattataatt tataatataa cttacaaata gctatttact atttaccata
     3961 attaagatgg tttagattag tcaaatagct atttttttag tttgagtaaa tattttaaat
     4021 ttaaaataaa agcaatattt aaatctatta tattatctac aaaaagtagg tgtgtcatat
     4081 tgagtttatt tagattttat tggccaaatt ggaggtgata taactccata tccacgagct
     4141 attgtatctt tacttggatt gatgatttta tgtggtacat tagcttttat aaatgttgag
     4201 tcacctggat ttaatctaac taccttttca cccatacaaa tatcaaccac accactcgtt
     4261 accaccataa tttcttctga tgcatgatat atataatctt tggcatcctc actttctggt
     4321 ttaagttcaa actctacaaa taaaattgaa ggtgtaaact cagttgtagg cattggagat
     4381 actatctcgt aaaacatttc actctttgga aatttcatca taactctatc ttcttttcta
     4441 atgactaaat catcagtatt attgtcatcc ataaacatat ataaaggagt gtctaaaact
     4501 tcagcgattt ttctcaatga agacaaggat ggttcaatag catctctttc tatttgagag
     4561 atataactta tagataaatc tgtcaatttg gaaacatcac ttaatgtttt tttcttgctt
     4621 tttcttatat ttctcatctt ttcacctagc ataagaacct ccaaataata aaattaatat
     4681 aaaatattcg cttaaaacaa agtaattata gcacaacata gaagtagtgg caataagaat
     4741 acatagtaga ttttgacaat taaaatttaa ttaaatccaa ataaaagtct atgaaaatga
     4801 attattgtta aattaataca aa
//
LOCUS       DBGJFU010000199         4797 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.203, whole genome shotgun sequence.
ACCESSION   DBGJFU010000199 DBGJFU010000000
VERSION     DBGJFU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.203"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(193..1236)
                     /locus_tag="ACOIAS_003595"
     CDS             complement(193..1236)
                     /locus_tag="ACOIAS_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGZ2703263.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1257..1703)
                     /locus_tag="ACOIAS_003596"
     CDS             complement(1257..1703)
                     /locus_tag="ACOIAS_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGZ2703264.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1881..3461)
                     /locus_tag="ACOIAS_003597"
     CDS             complement(1881..3461)
                     /locus_tag="ACOIAS_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGZ2703265.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            4260..>4797
                     /locus_tag="ACOIAS_003598"
     CDS             4260..>4797
                     /locus_tag="ACOIAS_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703266.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGLMIS"
BASE COUNT         1530 a          829 c          493 g         1945 t
ORIGIN      
        1 tttttacatt agtaaaggat aaattgtata aagattctgt taatttattt ttataatttc
       61 atctttctaa taaaaacaaa gaagaagact taaaaattta taagtcctct tcttttcaac
      121 gtgcattttt gtgctatatt taattaattc gacctttaat ttccatatcc ttcctatgat
      181 aacaaaactc ttttattttt ttaatgattc attatcattt aaaacttctt ttatatcaag
      241 tacttctttg ttatcatcac tcataacttc ctttattcct ttgaatgttc ctaggaaatt
      301 aactgcttct gaaggtaaaa ccagttttgt tgacttacct tcagccattt tttcaagagc
      361 ttccatagat tttaatgcta gaatattatt gtctatatca gcctctttca ttgctgaaaa
      421 aactaatttt aaaccttctg ctgttgccat ttgagttctt tttatcattt cagcttcacc
      481 ttgtgcctta gcaatagctg tctgtcttat agcttcagct tcaccttctg ccactaatat
      541 agcagattct ttttcacctt ctgctacacg taccatagct tcttttttgg cttctgctgt
      601 taatattgca gattgttttt caccttctgc ttgaagtatt gcagctgact tattaccttc
      661 tgcttgaagt attgcctctc ttctttctct ttctgctctc atttgttttt ccattgcaac
      721 ttgaatatcc tgaggaggca ttatattttt taactctact ctatttactt ttatacccca
      781 tttatctgtt gcttcatcaa ggatagttct catttttaca tttattatat ctcttgatgt
      841 taatgtttca tctaaatcaa gttcaccaat tatatttctt agtgttgtag ctgttaaatt
      901 ttcaatagca gctattggat tagctatctc aaacacatat ctaactgggt ctgtaacttt
      961 ataatataca actgtatcaa tctgcatagt tacattatct ttagttataa ctggttgagg
     1021 tggaaaatct attacaattt ctctaaggtc aattacatat gccattttat ctaaaaatgg
     1081 tattaagaaa tgtacacctg tttcagctac tttttgaaac ttaccaagtc tcattattat
     1141 acctacttta gattgtttta taactcttat acaagttaaa cttattgcta cgactactac
     1201 tattaataca atacttaaaa caattttagt acccatacta taaatcctcc tttattctat
     1261 tggtttgtca cattaactgt ggcgtctact tttttaacaa ctaattttac cccttcaatt
     1321 tttaatactt ctactgtaga acctttttct atcttttcac catctataga aatagccgtc
     1381 catgtttcat gttcaactac aactagacct gtgttatttg gagaaatctc ttctgtaaca
     1441 ataccttttt tagacatcat tgcttgtaaa ttagtgttag atttatagcc tttatctttt
     1501 tttactattt ttttagtagc tacgactaac attgctatag aaattactgc aaatattaaa
     1561 atttgtaaga aaatactctc tataaagcta cttaaaaata taagtattac agctcctaca
     1621 ctaaaccata tcaatgttaa gcttggtgtt aacatttctg ctattccaaa taagactgct
     1681 actatcaacc atattaactt catactatct accctctttt catatgaatt ataaaaccct
     1741 atataaatta tatgtttata caatgttttt gaatataaat ttaaattaca aaacaataaa
     1801 ataaattaat taattaatta atagtattat atcaacatac tttgttaaat ttgttacaaa
     1861 gtagtatcaa atatcaccaa ttatatatcc aacaactctt tcattgttaa tgaaatgtta
     1921 cttattaaat ccgtagcatt taccgtatac attgctttgc tcagtttatc tcctatatag
     1981 ccatgtatat atgctccact aactgtagct tccaatatat ccataccttg acctataaat
     2041 gaagtaatca ttccaagcaa acaatcaccc attccaccag ttgccataca gctatttcct
     2101 gtaggattta tatatgtttt tttaccatct gttatgacag tttgataacc ttttaaaagc
     2161 accaccagat tatatttttg agcaaattct tttgcaacat ctactctatg ttctcttata
     2221 tacccaattg aatctcctgt caacctggcc atttctccca aatgaggtgt aattacaaat
     2281 ctatttttcc attctaaaaa cttataacat ctgtccttca taacgttcaa tccatctgca
     2341 tctaatacta ttgggcaatt agaattttct actatcttaa gtaatttatc aaatgtttga
     2401 ctattatctc ccatacctgg accaaaacca attgcatcac tagaattaag taatttattt
     2461 aatctttctt cttcaaaatt taaagtcatt gcttctgtaa gtctaataga taccttttct
     2521 tgcacatctt cactactaat taatgttaca agtccactcc cactcttaac agcagattca
     2581 gttgctatga aacttgcacc ataaaatcct tttgaacctg caactataga tacttttcca
     2641 aaatcacttt taaaaccatg cttatctctt cttctaatat tattttttac aaaagtctta
     2701 tcagttatga actcttcatc atggtatttt cttgtaataa aatctggaac acctatcttt
     2761 tctacaacta catcacctat aagtgcgcta gtgtcatagt ttagaaaacc tcttttataa
     2821 aactcaaagc taatcgtttt gtttgctcta atacaagttc ctaatacatt tccattatcg
     2881 ctatctatac ctgaaggaat atctatagaa tacgtatttt tgctattatc gtttactatg
     2941 tcaataactt ctttaaaaat accagttata tcacgtttta atccagtccc aaatatagca
     3001 tctactacaa catcactttt agatactcta tctttaaaaa ttgttaaatt tttatttttt
     3061 ataatatttt gtttctcaac tttaataatt ttatctactt cattttcctc tccataattt
     3121 ttttcactta aatcattttt attaatattt ataatattaa ttcccatatt taaaagtaca
     3181 ttgtaattta ttttagagca ttcacttagt ttttcaagat tacctactaa aaatacgttt
     3241 acttcttttc catatgtatt tagatgtctt gctattccaa gaccatctcc tccattattt
     3301 ccaacaccac atacaactgt ataactatta tacatgtcta tatccatatt tttcattgca
     3361 gatataaccg cattttccat caatacaatc attggaatcc ctacttcttc tacacatgca
     3421 gtatcaacat ttctgcttaa tttagatgta cctattagca taaattatca ctcctataaa
     3481 gttaaagact tcttaaatac ttaccaaaaa tatctattga aatcttttta atcccataaa
     3541 tatcataaat aaaatcacta aaactaagag ttttgaaata atttaaaata acaaaaaatt
     3601 ttgaattaaa acaattattt aatttattat accatataat ttaagttatt atgttgattt
     3661 aaaacctatt tacactaatt atctaaatat ctctttattt ttataatctt ttatgactta
     3721 tttatatttt tcatttttat aaaaaaacaa taattaagaa tataactata ttctacatgt
     3781 aaaaattttt ttatttaaac tcaattttct gataatttga taatttttca aaatttatag
     3841 ttgactttta ttttttactg tattaaacta attactatac aaaacagaaa acaaaatttt
     3901 caaaattatt aaattaaaag gagaggtaaa tatgaagact ttattaaaca aacaagtacg
     3961 attttttagt agccaattta gttagggttt gtacctaagt taagacaaat tgtcataaca
     4021 tagatacaag ccccttagga ttatgatttt taaggattgt atctatgttg gctatagtaa
     4081 ttgttgttaa agtttttatt ttatcatcat aatatagagc cacatggatt atactgtgtg
     4141 gcttattcga atttttgcca tacagttata tatactgtgt ggttttttta tatcaaaatc
     4201 attttttaaa accaaatttt aaaaaccatt taaaatattt tcaaaggggt gttttattta
     4261 tgtcaggtat tatatctgta atgacgcaaa gtttaatttt atcgattatg gcactggggg
     4321 tttatataac ttacaaaatc ttagattttc cagatatgtc tgctgatggt agctatacaa
     4381 tgggtgcttc tattgtagca ttttctctta ctaatggtat ttctcctgta gtagcaactt
     4441 taatggctat cttatgtggt tgtactgctg gtcttgttac agggattctt cacattaagt
     4501 ttaagatatc taatttactt tctggtattt tagttatggg aatgctatac tctatcaatt
     4561 taagaataat gggaaaatca aatattcctc tatttagctt taaacactta ttcaatgggg
     4621 aaatatctcc tatagtttta gccttagctt ttgtattcat ctgtaaagtc cttttagatt
     4681 tattcctaaa aacaggtctt ggatacactt taaaaggtgt tggtgataac tctcaaatga
     4741 ttaaatctct aggtataaat ataggttcta taaaaatact aggacttatg atttcaa
//
LOCUS       DBGJFU010000200         4787 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.204, whole genome shotgun sequence.
ACCESSION   DBGJFU010000200 DBGJFU010000000
VERSION     DBGJFU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.204"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            207..1358
                     /locus_tag="ACOIAS_003599"
     CDS             207..1358
                     /locus_tag="ACOIAS_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGZ2703267.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRLSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1404..2579
                     /gene="thiI"
                     /locus_tag="ACOIAS_003600"
     CDS             1404..2579
                     /gene="thiI"
                     /locus_tag="ACOIAS_003600"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGZ2703268.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2735..3538
                     /locus_tag="ACOIAS_003601"
     CDS             2735..3538
                     /locus_tag="ACOIAS_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGZ2703269.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFSQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3781..4422
                     /locus_tag="ACOIAS_003602"
     CDS             3781..4422
                     /locus_tag="ACOIAS_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HGZ2703270.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1921 a          450 c          925 g         1491 t
ORIGIN      
        1 tttataatat atttttgtag ggtatagtat aagtatatta tattttacta taaaaatttg
       61 taataaagtg aaaaaactag cttttcatga aataatattt tataatttaa gattgtcttt
      121 ttaataaaga ttaacaaaaa aaatactata attagtggta taatagttag agttgaagat
      181 tttaaataaa ggtaggtgta attaagttgg aaatatactt agacaatagc gcaactacaa
      241 aaccttatca agaagttata gataaaatgg tttatgcact taatacggaa tatggaaatc
      301 cttcttctgt ccatagaaaa ggtgtagaag ttgaaaaagc tataaaagaa gtaagacaag
      361 atatagctaa atctctagga gcgaaagaaa aggagattta ttttacatca ggtggtacag
      421 agtgtaataa tactataata agaggtatta ctagtttaaa taaaaaaaga aaaaatcaca
      481 taatatctac taacatagaa catccttctg ttttaaatac attaaaagat ttagaagaag
      541 atggttttga agtaacttat ttagaggtcg gaaaagatgg gaaaataaat atagaggatt
      601 taaaaaatgc gataaagtca actacatgct tagtaagcat gatgcatgtt aacaatgaaa
      661 taggaactat acagccaata ggtgaagttg gaaagtattt gaagggttta aaagaaaaaa
      721 tttatttcca tgttgatgct attcagtcat atggaaaaat taattttaga ctatctaaat
      781 acaatataga ttttatgagt gtaagtgcac ataaatttca tggtccaaag ggaattggat
      841 ttatgtatat aaaagaaaat aatcgcctta agcctatgtt aacaggtggt ggtcaggaaa
      901 ttggaattag gtcgggaaca gaaaatgtac ctggaatata tggtcttgga gaagctgtta
      961 gaatactaaa caaagattta gatgttgtta tttcgaaagt aaataactta aaaaatatat
     1021 taaaaaatga aataattgat aatatagaag atataaaaat taattctcca gaagatggag
     1081 tatgtcatat attaaatgta tcatttagag ggacaaaagg agaagtttta cttcattact
     1141 tagagcaaaa gggtatttat gtatcaactg gctctgcatg ttcttctaaa aagaaaggta
     1201 gttacgtatt aaatgctata ggtcttacaa atgaagaaat aaatggaaca ataagattta
     1261 gtttatctga tatgaataca gaagaagaaa tgttagaagc tgtgaaagtt ttgaaagaat
     1321 caatttgtga cttaaggtca ataatgaaaa gaaaataaag aatactgtat agatagtgat
     1381 aataaattag tttaagaggt gaaatggtgt ataatatatt aatagtaaag tatggagaaa
     1441 ttggagtaaa aggaaagaat agatatatat ttgagaatag gcttataaga aacataagaa
     1501 atatgttaaa accaattgga agatttaatg tatataaaga gtatggaaga atatatgttg
     1561 atttagaaga ctatgattat gaagaagtaa ttgaagaagt tagaaaagta tttgggatag
     1621 tgggtgtgtg ccctggagta agagctaaaa aagattatga tacattaaaa gaaatagctt
     1681 taaaaatgtt agaagaaaaa atagaggctg gatataaaac ttttaaggta gagtcaagaa
     1741 gaggagataa gtctttcggg ttgacttctc aagaaatgag tatggatata ggtggatact
     1801 tattatcaaa ggtaggagat aggattaatg ttgatgtaag aaatccagaa gttaaaataa
     1861 agtgcgaata cagagaattt catactatgg tctatagtga tactatacct ggatatggtg
     1921 gattgccact tggaacgaat ggtagagcta tgtctctttt atcaggaggt atagatagcc
     1981 cagtagctac atggatggtt gcaaaaagag gtatggaagt agaggcagtt cactttcata
     2041 gttatccatt tacaagtgaa aggtcacagg aaaaagttaa ggatttagct aaaatactgg
     2101 caaaatactg tggtagagta aggcttcata aagttaatat cttagaaata caaaaagcaa
     2161 ttggcgagaa ttgtaatgaa gaagaagcta ctattctttc aagaagattt atgatgagga
     2221 tagcacagag actttctgaa aaaagacatt gtgatgcttt aattacaggt gaaagtattg
     2281 ggcaggttgc atctcaaact atacaaggac ttacttgtac taatgccgtt gtagacttac
     2341 cagtgtttag accacttata gcaatggata aatcagatat agttgatata gccaaaaaaa
     2401 tagggacttt tgaaacatct atattgcctg aagaagattg ttgtagtgta ttttctccta
     2461 gaaagcccgt cactaaacct agattagaga aaatagaaaa atctgaaaca gcacttgaca
     2521 ttgaaaaatt agtacaggat gctattgaca aaatagaggt agaggatatt gagttttagc
     2581 atacaagtta tttaattact aaattgaata ttgagtatat tagtagttct gtaagtatat
     2641 tagtagttct gttgaaagga tttatgtaga caatttatta atatgtagta tttaaaaata
     2701 agtactataa ttttatttaa tgggaatgat tattatgaat aaaaagggtt ttacactaat
     2761 tgaattgtta gtagttatat ctataatagg cattttagtt atagtagctg ttccagcatt
     2821 gtttagaaat atagaaaaaa gtaaagcagt tacatgtctt tctaatagag aaaatataaa
     2881 gactcaaatt gttattgcaa tggctgagga atcaagtaaa ggcaagaatg aagtcatgaa
     2941 agaggtatta gaaaacaaag atggtaagta ctttgaaaca gaaccaaagt gtaagtcagg
     3001 tggaatatat tcagcaacgt ttgatgatgg ttatgatgga ataactggaa tagaaagcat
     3061 tgcaaaagtg tatgttactt gtacaaaaca tccagatggt gttgaaatgg ctagggatat
     3121 acatcaaagt atgaaagatt tgattgcatc attttcacaa gacccttcta taataccagg
     3181 agcctcaaag ggtaatgatg attttagaaa atatttatta gacaataaat ataaaaatgg
     3241 gtggcctaca attccagatg aatttaaggc aaaatatgga ttaagtaagg atacactata
     3301 tatacaacca tatgcatata gtcctactaa atctgatgct actgtagttg tatttgcaaa
     3361 taataagact gggggtaatt ggtatacttc cctagtttac gattatgatg aaggtagatg
     3421 gtataaaggt aaaaatggta tttctgttgc aggtaggtca tgggatgttg acacagatag
     3481 tgttaagtct gtaaaaacag agattcattc taaagaggga tggggtcctt taaattaata
     3541 tatcatgttt tattaatctg gtattgatta aactatagaa aagagcagtt tctatagatg
     3601 acgaatacca gattttcatt tatatatata attgagatat attaaaagtg tttatatttt
     3661 taagtcttca catacaagct aaaaataatt ataataatat tgtaaagaaa tttaattata
     3721 agattaattt tctgaagatt tagtatttgt actaagaaat acataaaaag gggaatagat
     3781 atgaataata attttcaaga caaccaaaca ataaatttaa tacaaagtcg tagaagtata
     3841 cgaaaattta cgacagaaca aattagtgat gagcaagtaa acacattatt acactgtgca
     3901 tttgcagcac cttcaggatg taataaacag ccttggcaca taactgttgt gcaagaccag
     3961 aaacttttaa aagagattag tgatgatact ttatctagga ttcatgaggt gagtaatgtt
     4021 gagataaata aaaactttaa gttattttat ggtgctccaa ccgttttatt tatttcttat
     4081 gatgaaagta gttcatgggc tccatatgat ataggaatac ttacagggaa tataacaaca
     4141 gctgcacaag cactagggct tggaagttgt ataataggaa tggttagagg tttatttaca
     4201 cctgttgaac aaggggatat tgaaggtctt gtgagtgtat tggataaaga agatgtaaaa
     4261 gaatcagagt caataaagat gaagtttgat acaaataaaa aatacagaga actacttgat
     4321 ataccagaag ggtattctgt accatttggt attgctgtag gtattccaga tggaaattta
     4381 ccaaatgcta gagaggtagt ttataaagtt tccagagttt aggataaagt attgcaagaa
     4441 ccaatcagtt acttgaagaa ttaaaccagt aatatacaaa taaattgatg gaagaggaag
     4501 ctatttaaaa agtagtttag atttttcgtt gtaaaataat taaataaaga ataaggtgat
     4561 aagtatttta tttattatct tattcttttt tatttttgtt ttgtagaatg ataaaaataa
     4621 ggattaagtg taataaaata gaaaacaagt atgttaattt taagtatatg ctttaaaatt
     4681 tatccaagtt aaaaatttaa gatatctttt cagtataatg gaaaaaatgt caaaatattg
     4741 aaatagggaa aaaatagtga tataataaga aaaaatagag aaaaaaa
//
LOCUS       DBGJFU010000201         4734 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.205, whole genome shotgun sequence.
ACCESSION   DBGJFU010000201 DBGJFU010000000
VERSION     DBGJFU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.205"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            189..1187
                     /locus_tag="ACOIAS_003603"
     CDS             189..1187
                     /locus_tag="ACOIAS_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HGZ2703271.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1549..2076
                     /locus_tag="ACOIAS_003604"
     CDS             1549..2076
                     /locus_tag="ACOIAS_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HGZ2703272.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            2103..2699
                     /locus_tag="ACOIAS_003605"
     CDS             2103..2699
                     /locus_tag="ACOIAS_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HGZ2703273.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2764..4347)
                     /gene="asnB"
                     /locus_tag="ACOIAS_003606"
     CDS             complement(2764..4347)
                     /gene="asnB"
                     /locus_tag="ACOIAS_003606"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HGZ2703274.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         1687 a          723 c          663 g         1661 t
ORIGIN      
        1 taaattttag agtaattatt acattaaata aaaaattact aaatatcaaa ataacacagg
       61 gaaaacaatt tcaaaactaa atttttaacc ttaaaaattt tgtataaaaa atataacctt
      121 atatgctaca tattatttct aaatgaaaaa ttaccataat aatttaataa ttacaggagg
      181 gtattgatat gaaaatacta gtatttggag cacgcgatta tgaagaacca gtaataaaaa
      241 aatggtctga agaacataag gatgttcaag tggatattta tcctgaaaac atgactgaag
      301 aaaatgtagt taaagctaaa gggtatgatg gtatatctat acaacaaact aactatatag
      361 ataatcctta tatttatgaa actttaaaag atgctggggt taaagttata gcttcaagaa
      421 ctgcaggggt tgacatgata cattttgatt tagttaatga aaatggactt atcgttacaa
      481 acgttccttc ttattcacct aatgcaatag ctgaattagc tgttactcaa gctatgaacc
      541 ttttaagaaa gactcctcta gtaaagaaaa aagtctgtga aggtgattac cgttggatag
      601 ctgaacttct tggaacagaa gttagatcta ttacagttgg tgttataggt acaggaaaaa
      661 taggtgctac ttctgcaaaa ttattcaaag gcctaggagc taatgtaatt gcatttgacc
      721 aatatccaaa tagtgattta aatgatatat taacttacaa agattcttta gaagaccttc
      781 taaaagaagc tgaccttata acattacata ctcctttact tgaaggaaca aaacatatga
      841 taaataaaga tactctagct ataatgaagg atggagctta catagtaaat actggccgtg
      901 gtggtttaat taatacaggg gatttaatag aagcactaga gtcaggaaaa attagagctg
      961 ctgcccttga tacatttgaa actgaaggat tgttcttaaa caaaaaaatg aatcctggag
     1021 aattaactga cccagaaata aataaacttc tttctatgga acaagttata ttcactcatc
     1081 accttggttt cttcactagt acagcgattg aaaatatagt ttattctagt ttaagtagtg
     1141 ctgtagaagt tataaaaaca ggaactgcta ctaatagagt aaattagtat tttataaata
     1201 tattttttca ttaattagct attatctaac aaaatagcct ttaatttccc aaatataaga
     1261 gagtcagctc agtatatatt gagtagactc tcttatatta ttttttatat taaacttgta
     1321 ctcttcttat tcataccaaa ttaaatttat attaacttac tctaactact cctactctat
     1381 acattttacc tatttttact tttctaatta tatacatttg aatgggtaca atctgtattt
     1441 tacaaaaaaa acacctaaca aactatgaaa attgtatgat taatattaat atatttataa
     1501 gaaaacataa caattatgtt agctctggat ttttagggag ggagtatatt ggaaaaaatg
     1561 aataaagtta cttctaaaaa aactacctat attgttactt cagcattatt tgcatccata
     1621 atatgcctga caatagcata tatattacat attccagttg gtggaaacaa tggatatgtt
     1681 catataggag atgcttttat ttatctagct gcaactattt tgccaacaaa ctatgctata
     1741 gctgcttctg caattggtgc tggtctagct gatttatcta caggtgctgc tatctgggta
     1801 ataccaacta taatcataaa accaatactt gtattatttt tcacttctaa aagtgacaag
     1861 ataataaata agagaaatat agttgcttcg gttgtagcag gtatagtagg attagtttta
     1921 tacatgtttg ctgaaggtat aattattgga agttttacaa gtgcatttgt aatgagctta
     1981 cttggcttat tacagccaat aggaagtttt attgttttta taatcttagg tatggcactt
     2041 gataaacttg attttaaaaa acgttatttt aattaagaat ttaaatttat ggagggaaaa
     2101 ttatgaatat cttatataca attgaaaata gtatctatgt aaatataact aatacatgtc
     2161 catgtagttg tgttttttgt ataagaaacg aaaaagatga agttgcaaat agtggtagtt
     2221 tatggttgga gcatgagcca agtgtagatg aagtaaaaga agccttcaat aaatacaatt
     2281 tagacgacta tgatgaaatc gtattttgtg gatatggcga accattaatg agaataaatg
     2341 aattgataga ggtagcaaag tttataaaag aaaaaagtag cataaagata agaataaata
     2401 caaatggtct aagtgattta atacataaca aaaaaacagc tattctgtta aaaaatgtta
     2461 tagatgcagt atcaataagt cttaatgccc caaataaaga agcttataat agagttactc
     2521 aaccaaagtt tggagaaaaa tcatttgatt atatgttaga ttttgcaaga gattgcaaaa
     2581 aatatataaa agaagtagct ttttcagtag tagatgaaat atctccagaa gaaatagaag
     2641 aatctaaaca actggcaaaa aaacttgata taccattaag ggttagacat aagaattagc
     2701 aagtaaaatt ataaaacatt aagaatcacc tgaatggtct ttcaggtgat tcttcctctt
     2761 gtattaacat tttacaaaat agatttcata ccacactagg aatgtgtaaa tcatccaaat
     2821 ctttctccaa ttatcagagt ttccactaat gtagtcatta aaaatctcat taatctcatc
     2881 tatgttaaag aatttatctg ctatctcact attgaattta tctcggatat ccttattata
     2941 ttggtcatca gccatccata tacgaattgg cacaataaat cctaatttat ttctaaaagc
     3001 aatctcttct ggtaatgttt ttgatgcagc tgtacgaaag gcaattttat tctcttttcc
     3061 tttcacttta tattctgatg gtattctgga tgcaatatca aatattcttg tatctgtaag
     3121 aggcattctg acttccaatc ctactgcagt actcatttta tctatattaa aataaatatc
     3181 tccttccaac catatttgaa tatctacatt tgacatgtta gtaagtgtat ctagttcttc
     3241 tgtttcttca taaattcctt ttaccacttc aataggaagc acatcaggat tgtatttctt
     3301 aagaattttt cttttctcac cctcattcat aatgtttgta tttccaatat aaaatgtctt
     3361 caaattctct ccataacact ctgccttatg gtatatatta tatccaccaa aaaactcatc
     3421 tgctccttct ccagagtaac agatatttgt gttttcagct gctgccttgc atccaatagc
     3481 aaaaacgata gcagatgcat ctccagttgg ttgctccata tggtacatta cataaggtac
     3541 tgacgcaaaa tactcttcag gtgtaattat atatctcgaa tttttacacc caagaatatt
     3601 agctgtctgt tgtgctaatt ttgattcatc aaaacgctct tcctcataac cacatgaacc
     3661 tgttttttct atatctgaca ttgccaatat ataagatgaa tcaacacctc ctgataggaa
     3721 taactctgca gtttcattct ctgattttac ctctggcata attttctgaa gtgttgtatg
     3781 aatttcatcc gtccattcct caatagactt gctgtggtca ggatgaaatt caggtttcca
     3841 ataacgacca atacatactg ttttattctg atagacaagg taatgtcctg gcataagctt
     3901 ctttactcca cggaagaatg tattttcacc tgctacatat gtgaaagaca tataaatctg
     3961 taacatgtct tcatttaact ctttcacaaa tcctggttgc tccataatct tacgaataga
     4021 tgaaccatag agaagcttcc cattttcagt ctcataataa tagaaaggtt ttaccccaaa
     4081 ctggtctctt aggcagaaga gtttcttttc cacagtatcc cataatgcaa aagcaaacat
     4141 tccatgcatg tgatttgcca tatcatatcc ccatgtctta tatgcttcca ctaaaattgc
     4201 tcgttcacgc tcatctctac ataatgaaga atcgattcca agttcttcac aaagaacttt
     4261 ccagtttcga atatgcccat tgtattctag aatttttatc atatatgtgt cttttcgtgt
     4321 taaatacttt tcttctaaat ttatcatatg tgtatccttt cgtattaaaa catttaactt
     4381 ctaaatttat taaatgttta tatatattta ttcacttata atcactgaaa aatcgacttt
     4441 tttcaaagta aattcttttt gaattagtac taatctgctc tttattaatg gcataaatat
     4501 gattatcaag tattatcagt gttataacat tgaatttttt attcttcata aatattgccc
     4561 cctttatatt accaactaaa tataatatat cttatgttac ttacatattc tttaagaaat
     4621 tctgaagatt ttataaagtt ttttgtatta taattaatta aaaataaaat tctagttata
     4681 aaacatattt tccattaaac aaaaaaatag ttaaaataaa ttcataacca cata
//
LOCUS       DBGJFU010000202         4709 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.206, whole genome shotgun sequence.
ACCESSION   DBGJFU010000202 DBGJFU010000000
VERSION     DBGJFU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.206"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            236..1375
                     /locus_tag="ACOIAS_003607"
     CDS             236..1375
                     /locus_tag="ACOIAS_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGZ2703275.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1401..1589
                     /locus_tag="ACOIAS_003608"
     CDS             1401..1589
                     /locus_tag="ACOIAS_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703276.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            2374..>4709
                     /locus_tag="ACOIAS_003609"
     CDS             2374..>4709
                     /locus_tag="ACOIAS_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HGZ2703277.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKPSDKSKTKTKAEEKIAEVTKLVGKVTADNLEASKVTLKAATDAIEEA
                     RKAGWTESEVQSFKGYGDIAKVQSEITELESQAKEAAKTKAEEKIAEVTKLVGKVTAD
                     NLEASKVTLKAATDAIEEAKAAGWTESEVQSFAGYEDIATVQGEIAALESSQAKEEAK
                     TKAEEKIAEVTKLVGKVTAENLEASKVTLKAATDAIEEAKAAGWTESEIQSFVGYEDI
                     AKVQSEITELESQAKEAAKTKAEEKIAEVTKLVGKVTA"
BASE COUNT         2069 a          496 c          796 g         1348 t
ORIGIN      
        1 gatattgtta agacattgtc gagatattat aatatgtaac taatgtatta taaataatag
       61 ttgtaaactt cctgtgacat atattttaaa tgcttattct taaatttttg cattatttat
      121 taaaatttat tatgctttaa cgctaaattg agtttgataa ttataaaaca aataatcaaa
      181 aatagtgtaa aaattaatac tttttgttaa tattaataga taaagataat ctaaggtggt
      241 gattaaactg acaatagaag aatacaaaaa caaatacttt actggaaata aaatcgtact
      301 ttctataaat agttttgata ataaagaact atactatcat gaaactgaaa atatattaaa
      361 tttaataaat gaaatagata aattaacatc aagtaagata tttaaaacaa tagaagataa
      421 aaatgatttg gcactagagt cttattattc tagtgtaata gaaggtgctt tttcaactag
      481 aaagattgca aaatctataa ttagaggaaa gatgaaacca agtaataaaa gtgaatatat
      541 gatttacaat aatcatagag ccttagaata tggattagac aacttagata aattatatag
      601 tcacaaattt atatatgatt tgcatcatat attgggcgaa aactgcttgg attcagaaga
      661 atatgagtat agaactgaaa aagtatatgt atgtgattca aaaggtgaga ttatacatac
      721 agggcttgaa cctttgaaga tatatgactt tatgagtaaa ttaatagatt ttatggaaaa
      781 tagcaaagta agtaatttaa ttaaaagtgc tataattcat ttttactttg tatatgttca
      841 tccttttagt gatggtaatg gaagaacatc aagagcctta tcatatctat atttgattga
      901 taaaggatat gatactttca aggatttttc aatatcatat atgatatcta aaaacagaac
      961 taagtattat aaggctatac ttgatgtaga aaacaaagga aataatttaa ctgtatttat
     1021 agaatttatg ctcaagtcta taatacaatc tattaatgag atgcgtaata tgcatgatag
     1081 aaaatcttta gaaagtatat taaaagaaga attgtttgaa aatgacataa ctctatcagc
     1141 aacagaagaa catatattaa aatatatttg caataaagat aactattcta tgacattaga
     1201 aaattatata aaaaagaata aaagtagata cttaaaggct ggaataaagg aaattgaatt
     1261 agttgaccag ttgatggaag tgtttaataa tcttgaagag atagagattt tgtctaaaga
     1321 aaaaaatata tataaagtta atgataagta cttaaaaatg cttgatatag actaatttag
     1381 ataatctgga gagggaatat atgaataata agaaaaaggc atttttgatt tctggggcta
     1441 taaacgtaat ttcattaatt attgtttttt cattgtttca ttataatatt atagaaaata
     1501 gtacacaaaa tatgataata actgtaatct taatttttat ttttgaaatg ataaaaataa
     1561 aaatcataga gaaatactat aaactataaa caagaaatat cttttctaag agcatattgt
     1621 acaatatgct cttaatttag ttatatagat ttaatataaa taatgtattt tttgtagatg
     1681 atttatgtaa gatttatgac caatttgtgt atgatttgtg ttctttaatc atgacaatat
     1741 tatattgtaa aaaataaatt taataaaaaa tgacttggaa tttatattct aggtcttttt
     1801 tatataaaaa tttaataagt ttcaaatgtt ggtaactttg acagtagaaa acgtctattg
     1861 agataacgag gattctaatt gagggggaga gatattagca cttaaagtag tatatgcaac
     1921 agatataatc tttacaaaag taatttgaat taaaatgtaa atataaagaa taattaaaaa
     1981 aatatcgcaa ttatttgttt ttcatatgga taaaattggt tatttaatat ataattacaa
     2041 gtataaataa taaaatttat ttgtaaaaag agtagcactt ttaattcata aaggctactt
     2101 tttttataca aaaccctatt ttttataaca attcattaac ataaaaatca aacttacctt
     2161 aaaatataaa tttacaaatt tttcaatcaa taagaaaatt tctttattcc ttacataatg
     2221 actgcaatgt aacaaaataa ttacaaatta attatgaaat cattatagtt agaaacctct
     2281 ttaaaattag atattaagta tcctttagaa ttagaacggg aacaaagtta aaaaattaag
     2341 taatatcaaa tggacaaggg ggaagaaaat aaaatgaaaa taccaaagaa gattgctgca
     2401 atgcttacag ttacaatgat agctggaagt tcaacagcag gcatagctag tgcacaaact
     2461 gtggcaacaa atttaacagg gcaagaaaga tatgaaactg ctgttaaaat aagtcaagat
     2521 ggatggaaaa atgctgatga ggtagtaatc gtaaacgact catctatagc agatgctcta
     2581 tctgcaacac catttgcaaa agcaaaaaat gctccaatac ttttaacaag taaagataaa
     2641 ttaaatgata agacgaaagc agaaatacaa agattaaaag ctaaaaaagt atacttaata
     2701 ggtggaactt ctgtattaag tacaaatata gaaaaagaaa taaaagattt aaaaatatct
     2761 tttgagagaa tctctggagc tgagagatat caaacatcct tagaattagc aaagaaatta
     2821 gatgctataa gtgatgttaa gaaaatagct gtagttaatg gtgaaaaagg acttgcagat
     2881 gctgtaagtg taggagcacc agctgcacaa aacaatatgc cagtaatact tgctgattct
     2941 aaaaatggaa cagcagttgc agacaaattt ataaaagatg ctggaataac tcaatcttat
     3001 gtagtaggtg gagagagttc aatttctgaa gctgttaaaa ataaactacc aaatagtaca
     3061 agactaggtg gaacagatag aaatgataca aatgcaaaag taataaaaga attttataaa
     3121 aagacagatt taaaaaatgc ttatgtaact aaagatggta tgaataaaca agaccaactt
     3181 atagatgcac tagcagtagg tgtattaggt gctaaaaatc aatcaccagt agttttagtt
     3241 ggaaagaact tatcagcttc acaaaaatct ctagttaact ctaagagttt tgataagata
     3301 actaaagttg gtgggaatgg taatgagaca gcatttaatg aaatgaagtc attacaagaa
     3361 gtaaaaacag ttgaagctaa aacaatttct gaattaaaat cagctataga taaagctact
     3421 gctaatgatg ttataaactt taagccaact agtgaggtta aagaagcatt tacaatacaa
     3481 actgataaag cagtaacagt aaacttaaat ggtacatata caaagactgt aactatcaac
     3541 atgcctaatg gagatgtaaa taactatgca aaagtggatg atgtagttat agatgatgta
     3601 aaagatggaa cttttgttaa ctatggaaaa ataactaact taaaggttaa tgataaaaat
     3661 ggagccaaga tagaaaataa ttctaaaggt gagattggta gtttaacagt agcatctgga
     3721 gcaagtcaag taaaagtgac taatggtggt aagataacta cagttacaaa taattctaaa
     3781 gggactacta tagataataa aggtacaata agtagtgtta agggagataa ttctccaaca
     3841 ataagtggaa atagtccaag ttctaactct tcaggaggaa gttcatcaag tggaggctct
     3901 tcacatggag gaggaagttc ttcatcaaaa ccaagcgata agtcaaaaac aaagacaaaa
     3961 gcagaagaaa aaatagcaga ggtaacaaaa ctagtaggaa aagtaacagc agataattta
     4021 gaagcatcaa aagtaacatt aaaagcagca actgatgcaa tagaagaagc aaggaaagca
     4081 gggtggacag aatcagaagt tcaaagcttt aaaggatatg gagatatagc aaaagtacaa
     4141 agtgagataa ctgaattaga atcacaggct aaagaagcag caaagacaaa agcagaagaa
     4201 aaaatagcag aagtaacaaa actagtagga aaagtaacag cagataattt agaagcatca
     4261 aaagtaacat taaaagcagc aactgatgca atagaagaag caaaggcagc aggatggaca
     4321 gaatcagaag ttcaaagttt tgcaggatat gaagatatag caacagtaca aggtgagata
     4381 gctgcattag aatcatcaca ggctaaagaa gaagcaaaga caaaagcaga agaaaaaata
     4441 gcagaagtaa caaaactagt aggaaaagta acagcagaaa atttagaagc atcaaaagta
     4501 acattaaaag cggcaactga tgcaatagaa gaagcaaagg cagcaggatg gacagaatca
     4561 gaaattcaaa gttttgtagg atatgaagat atagcaaaag tacaaagtga gataactgaa
     4621 ttagaatcac aggctaaaga agcagcaaag acaaaagcag aagaaaaaat agcagaagta
     4681 acaaaactag taggaaaagt aacagcaga
//
LOCUS       DBGJFU010000203         4695 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.207, whole genome shotgun sequence.
ACCESSION   DBGJFU010000203 DBGJFU010000000
VERSION     DBGJFU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.207"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..609
                     /gene="bclA3"
                     /locus_tag="ACOIAS_003610"
     CDS             <1..609
                     /gene="bclA3"
                     /locus_tag="ACOIAS_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HGZ2703278.1"
                     /translation="PTGATGATGVTGATGPTGPTGATGATGATGASAIIPFASGIPLS
                     LTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITSISA
                     YFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGI
                     VTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            778..1539
                     /locus_tag="ACOIAS_003611"
     CDS             778..1539
                     /locus_tag="ACOIAS_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HGZ2703279.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1848..2126
                     /locus_tag="ACOIAS_003612"
     CDS             1848..2126
                     /locus_tag="ACOIAS_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HGZ2703280.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2419..2994
                     /locus_tag="ACOIAS_003613"
     CDS             2419..2994
                     /locus_tag="ACOIAS_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HGZ2703281.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(3100..3777)
                     /locus_tag="ACOIAS_003614"
     CDS             complement(3100..3777)
                     /locus_tag="ACOIAS_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HGZ2703282.1"
                     /translation="MNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANV
                     LCHMGYASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFR
                     FSNYLNKESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVL
                     SFYNRAKKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYE
                     ILKNLEK"
     gene            complement(4001..4630)
                     /locus_tag="ACOIAS_003615"
     CDS             complement(4001..4630)
                     /locus_tag="ACOIAS_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HGZ2703283.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         1731 a          605 c          756 g         1603 t
ORIGIN      
        1 ccaacaggag caacaggggc aacaggagtt acaggagcaa caggcccaac aggcccaaca
       61 ggagcaacag gagcaacagg agcaacaggt gctagtgcaa taataccttt tgcatcaggt
      121 ataccactat cacttacaac tatagctgga ggattagtag gtacacctgg atttgttgga
      181 tttggtagtt cggctccagg attaagtata gttggtggag taatagacct tacaaacgca
      241 gcaggaacat tgactaactt tgcattttca atgccaagag atggaacaat aacatctatt
      301 tcagcatact tcagtacaac agcagcactt tcacttgttg gttcaacaat tacaattaca
      361 gcaacacttt accaatctac tgcaccaaat aactcattta cagctgtacc aggagcgaca
      421 gttacactag ctccaccact tacaggtata ttatcagttg gttcaatttc tagtggaatt
      481 gtaacaggat taaatatagc agcaacagca caaactcgat tcttactagt atttactgca
      541 acagcttcag gtctttcatt agttaatact gtagcaggat atgcaagtgc aggaattgca
      601 ataaattaga ttagataaga aaaggcttct aatatcgtat tgatattagg agctttttta
      661 agaatgattg atgaacatag ttttaatatt aattagtgcg catattataa aatactatta
      721 tataatataa aataataaca ttaaattgga aatatagaat tatctagggg tgatactatg
      781 aaaaataaga ttgaaatatc aatgaacaac tttgtaaaaa attatcataa atcaaataat
      841 ataactacaa agtggcaaga acttttgttt gcttatgcat ctgctgaaga tgatatgttt
      901 cagaagctaa aaagtgttgt aggaacatct catgctatgc caaaagattt tctggaaaat
      961 ggaaaaacta taattacata ttttatacct tttgatgaaa gtgtaaataa tagtaatatt
     1021 ggagggtttg aatgttctga aatatgggca agagcgtaca tagaaactaa tatgttgatt
     1081 tcaaatttaa atgatttctt atgtgaagaa ctagagaaga taggggctaa ttcatatgct
     1141 attcctgggt cgtataattt tgacagtgaa aaattggtaa gtgactggtc acaaagacat
     1201 gtagcattca tagcaggtct tggaactttt ggtttaaata atatgcttat tacagaaaaa
     1261 gggtgttgtg gaagaatagg aagcataatc acaagtttag aattagagcc aactaaaaga
     1321 ccagaaaatg aatactgctt gtataaacat aataatagtt gcaaaaaatg tgtcaataaa
     1381 tgcgtgaaag aagctttaaa agaagatgga tttaatagga ataagtgtta tgaaatgtgt
     1441 atgataaatg aaaaaaaata cacttatata ggtggatgtg atatttgtgg aaagtgtata
     1501 gttggagttc cttgttcagt aataaaccca tcaagataat ggatttaagt tgactaataa
     1561 ttttaaagat agtttataat gaaaaaattt tagttatcaa aataaatata atataaaaac
     1621 taaataaatg tgtttaaaaa atggataaat aatttgtgtt gattgacaac cagttatact
     1681 gttgatagac ttatatgtat cgctgtgata tataaggagg gttgagattt tatgtttgat
     1741 ttaatcttta tttttgcata gcaagggcta ttgcaactat gaaatttaat atataaatag
     1801 ccctttgcta ttcctaatga aaaattacta tttaatagga gagcaaaatg agctataaaa
     1861 aagcaataca tatattacca gaagaattac ttgaattaat acaggaatat gtagatggtg
     1921 aatgtatcta tattcctaga aaatctaata ataaaaaaga atggggaagt aatacaccta
     1981 ctcgtgagga gttgtcaatt agaaatatgc agatttacga agactatcaa gttggatatg
     2041 atttgcaata tttatcggaa aagtattatt tatctttgaa aagtatccaa agaattgtat
     2101 tacaagaaaa aagaaaagat atctaaggat gagccagtat agttttataa ttatactggc
     2161 tcattttctt ttgataaaag tgtttgaggt atagtttctt ttaagtggat gatataattt
     2221 aagtaatttc aataacttat acagtagcta acaaagctgg ttgtaaagta tctaataaga
     2281 agtagtagct ttttatatgt ttgaaattaa aatttattaa aattaggagg tcttgttttg
     2341 aaaaaaatac ttgattatat tccacctttt cataactttc tattaattta gtaatcttat
     2401 agaaaggaaa ttgcatttat gaaaaaaatt acttgggagg tggaatatat ctcccagtta
     2461 cctgtaatta ggtattgtaa aaaatgtgga aaaaagactg aatatatttg ttcaggttta
     2521 tttagggtta atgctcaaca taaatatttg gatatatggt taatatacaa gtgttcaaat
     2581 tgtgattcaa catggaactc aactatatat tctcgtgtta atccaaaaag cttaaatcca
     2641 gaaatactag aacaattcca tacaaatgat tataatcttg ttaagcaata tgctatgaat
     2701 atggaacttt tacatagaaa tggagcagaa gttggtattc cagagtataa aatagtagga
     2761 caagaaatta atattaatga ccttacacaa gttcatatta ttagtaaata tccatgtcag
     2821 ttaaaggtat caacattatt acgtgagaaa ctaggtttat ctaggaaggt atttgacgaa
     2881 atgttggatt gtgggattat tagaagtaac tcaggagttg atttaaagaa aggtaaatta
     2941 aattatgaaa ttatgttaga tataggcaag gagaagcaaa agtcttcaaa ttgattagaa
     3001 taactaactt aatatatttt caggaacatc atttaaaaaa atagtgactt taaaaaattt
     3061 aaagccacta ttttttaata tgttttatct atataactat tatttttcta ggttttttaa
     3121 tatttcgtaa tagtgtgttg tggctatttc acttgactcg ccttttttac aaaaagataa
     3181 tttagatagt tttggaacta cacgttcaac gacaatttta ttacctatca ttttagattc
     3241 aagtatcttc aatgcttcta aaaatctctt atcctttttt gcacgattat aaaatgataa
     3301 aacgtagaca taaaggaata ggttataatt acggaaagga tattcaactt gcataaataa
     3361 tgtgccaatt ccatagtgac aagggccgat tggtttttta atagtccaat ggtcaagtag
     3421 aaaatctaca gctttgtcaa gttttgattc tttatttaga taattactaa aacggaatgc
     3481 atttaatata ttaagagttg gtagtggatt tgagtattca gtttctggac ctcgaccaaa
     3541 actaaattta ttgcaacgcc acccaccatc agtatactgt gtatctaaaa aatgttgaaa
     3601 cgttttttgc attctaatgt cagatgcata tcccatatga cagagtacgt tagcagcatt
     3661 tgcagtatga caagggtaaa tacctccatt tggatatagt ttgaaacgtc catcttctcg
     3721 ccatgtgcta aatatcaggt cagcacattc tttaagtaaa gtatcatcag ggttcacacc
     3781 taattctaat agataaagta cactatcaag agtagagaaa ggagaacctt ttattaaacg
     3841 cttatcaggt gtagtccaat agtctgcacc attatcatac ctgtgtgata atattatttc
     3901 aatatctgat aaagattgat tttctacatt cattttttac tccatttcat attatattct
     3961 attaaatcta tcaaaaactt tattataata ttatttatat ttataattga tttattttat
     4021 ttaaaattgc atttatagct gtatcaggaa caactgctaa atcttctgca ttgaagtaat
     4081 aaactatatt agaatcaaat aatatatttg aatttggagg aaattcatca tctccaaacc
     4141 aaagtataaa taccatagat gtattaccca tatatgtaaa cctataagct aaatctccca
     4201 tgtttacttt ttcagctcca attttctcca taactaactt atatttgtct atttgagatg
     4261 taaaaacttc tgaaagccta tatagagtcc tgtttaaaaa atttggataa taaacattgc
     4321 ctcctggtat ttctttaaaa gttacatatt tattagttct aggaatacct ttagcattga
     4381 ttaaatatct aactatcaag attttaagct caaagttatt gcaaaggcta ccattactat
     4441 tccaaacttc tccacttgga tagtctataa tatattgtct atcaaaaaaa ttgagtgtga
     4501 atctatttaa atcattattg aaaggtatat tacttaaatt actaattgta tgagggtcta
     4561 attctttgag tttttttcga acatagtcaa atggaacatt ataattattt tttgttatct
     4621 tttcatccat aattttttcc tccaatttaa acaattaaaa gtatatttat aaaaagtttt
     4681 cttattatta atttt
//
LOCUS       DBGJFU010000204         4645 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.208, whole genome shotgun sequence.
ACCESSION   DBGJFU010000204 DBGJFU010000000
VERSION     DBGJFU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.208"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     regulatory      188..292
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            502..2976
                     /gene="gcvPA"
                     /locus_tag="ACOIAS_003616"
     CDS             502..2976
                     /gene="gcvPA"
                     /locus_tag="ACOIAS_003616"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HGZ2703284.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            2976..4433
                     /gene="gcvPB"
                     /locus_tag="ACOIAS_003617"
     CDS             2976..4433
                     /gene="gcvPB"
                     /locus_tag="ACOIAS_003617"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HGZ2703285.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         1868 a          506 c          874 g         1397 t
ORIGIN      
        1 taaattaagt gaaaatgtta ttttacatta atatataata tctttgcata agagtgaata
       61 atataaaaga gaaataattg ataataaaaa cttaacaaaa aaattaatac ataaaaaaaa
      121 ataaatataa tgtgttgaca tttaattgaa atattcatac aataaaattg aaaataaata
      181 tttccaaatg agattagcga gagagtcctt tatacgaaaa ggcgccgaag aagaaattta
      241 agaaatgcca atttttagag aagctttcag gcaaaaatat cgctaattga tgggactctg
      301 gagagactta agttaagata ctataagtca ccgaagaagc aaaataatat taaattatta
      361 aatctttcag gtatacaaac agggatatat aggctaggat tattttccat gctgatatat
      421 ctttttttta tgaaaaagta caggaaataa tatcaaagtt acttaaatac agtaggtcta
      481 attttctagg gagggttata tatgaatgaa ttgaaaagag tatctttgta caatatacac
      541 aaagagcttg gagcaaaact tgttgaattt gctggttggg aaatgccttt agaatatgag
      601 ggtataaata aagagcatga gaaggtcaga aaaagtgcag gtatatttga tgtatctcat
      661 atgggagagg tacagataaa gggagcagag tcagaaaaat ttatacaaaa cctagttaca
      721 aatgatataa gcgcattaaa gataaatgat attatatata cacctatgtg ttatgaaaat
      781 ggtggagtag ttgatgattt attaatatat aaatttggtg aagaagatta tttattagta
      841 ataaatgcag gtaatataga taaagatgta gcatggataa taaaacaaag tgaaggatac
      901 aatgtagata taaaaaatat atctagtgaa gtgagtcaat tagcaattca aggacctaaa
      961 gcagaagaaa ttcttcaaaa aatcacagat atagacttaa actcaataaa attttataag
     1021 tcaattccat caacaaaagt atgtggttgt ccttgtttag tttcaagaac aggatatact
     1081 ggggaagatg ggtttgagat atactgtaaa aataaatatg tagaaataat ttggaatgaa
     1141 gttctaaagg ttggaggaga agatatatgt ccagctggtc taggttgtag ggatactttg
     1201 agatttgaag cagctctacc tctatatgga catgaaataa atgaacacat atctccaatt
     1261 gaaggtggtt tatctatctt tgtgaaaaca aataaagaat catttattgg caaatcaatt
     1321 ttatcaaaag aaaaagaaag tggagctaaa agaaagttag taggatttga aatgcaaggt
     1381 aaaggaatgc ctagaaatgg atatgatata aggattggtg ataaaacagt tggatttgta
     1441 acaacaggat gtgcatctcc aacgacagga aaaattttag gtatgggaat tatagattct
     1501 gaatatgcaa aagtaggaaa tgaaataggt atagcaatca gaaaaaaagt tgttcctgct
     1561 gtgatagtta agaaaccttt ttataaaaaa caatataaaa aagataatat tatattaaac
     1621 aaagaaaata aattttcata tatacctgct acaagtgaag ataaatcaaa aatgttaaaa
     1681 gtagttggat taaattcggt tgatgaatta ttttcagata taccagaaga agtaaaatta
     1741 aaaagagatt taaatttgga aattggaaaa tctgaattag aggtaagcaa aatagtaaaa
     1801 agattgtcag aagaaaattt aagtttagaa gacttgactt gttttcttgg tgctggtgca
     1861 tatgaccatt atataccatc aattataaaa catataactt caagatctga attttataca
     1921 gcatatactc catatcaagc tgaaataagt cagggaactt tacaagtagt ttttgaattt
     1981 caatctatga ttgcagaaat tacaggtatg gaaattgcaa atgcatcaat gtatgatgga
     2041 gcaactgctg caatagaagc atgtataatg gcaatgaatc agactagaaa aagtaaaata
     2101 gtagtgtcaa aaactattca tcctgaaact ttgtcaatat taagaactta cttacaatat
     2161 aaagattgtg agatagtaga aattgacttt tgtaatgaat atggcactac agatatagaa
     2221 aaattaaaag cttctgtaga caaagataca gcatgtgttc ttatacaaac tcctaatttc
     2281 tttggaatta ttgaagaaat ggaagaaata gaaaagataa ctcatgaaaa taaggctatg
     2341 ttaattatga gtgtagaccc aatatcatta ggagttttaa aaacaccagg tgagataggt
     2401 gcagatattg ttgttggaga agctcaatca cttggaaacc ctcttaattt tggaggtcct
     2461 tatgtaggat ttttagctag caaatctaaa tatactagaa aaatgccagg aagaatagtt
     2521 gggcaaagtt tagatgtaga aggtaaaatt gcatatgtat taactttaca aactagagaa
     2581 cagcatgtaa gaagagaaaa agcaacttct aatatatgtt caaatcaagc tttaaatgct
     2641 cttgtagctt caatatatat agctactatg ggtaaagaag gatttaaaga ggttggtatg
     2701 cagtctatga aaaaagcaca ttatacttac aataagcttg tacaaactgg aaaatataag
     2761 ccaatattta aaggtaagtt tttcaaagaa tttgctgtac aaggaaatct taatatagaa
     2821 actataaatg ataaactctt agaagaaaat atattaggtg gttataattt agaatacaat
     2881 tatccagaat taaaaaattc aactttgctt tgtgtgactg agaaaagaag caaagaggaa
     2941 atagataagt tagttggaat aatggaggga ttataatgaa agaatacaat agtttgttga
     3001 tagatatatc taaaaaaggt aggaaagcat actcacttcc aaagttagat atagatgata
     3061 taaaaataga aaatatgatt gaccaagata tggcaagaca aagtgagttg aatttgccag
     3121 aagttggtga attagaatta gttagacatt atacattatt atcaaataaa aactttggag
     3181 ttgatacagg tttttatcct ttgggttctt gtactatgaa atataatcct aaaataaatg
     3241 aagatatggc agcacttcca aactttactg gaatgcatcc ataccaatct tcggatacag
     3301 ctcaaggttc tctttctttg atgtatgatt tatcaagaag acttgcagaa attacaggta
     3361 tggatgaagt tacactacaa ccttcagcag gttctcatgg tgagtttaca gggcttatga
     3421 ttataaaagc atatcatgaa aatagaggtg accataagag aaccaaagtg ataattccag
     3481 attctgcaca tggaacaaat ccagcaagtg cagctatggc aaactttgat gtgattcaaa
     3541 tagcatctga taaaaatgga gctgtagaca taaatgcatt aaaagaggtt ttgaatgatg
     3601 aagtagcagc tcttatgctt actaatccaa gtactcttgg tttatttgaa aaaaatataa
     3661 aagaaatagc tactcttgta catgaagcag gaggactttt gtactatgat ggtgcaaaca
     3721 tgaacgctat tatggggata acaagacctg gagatatggg gtttgatgtg gttcatctta
     3781 atcttcataa gactttttca actcctcatg gaggtggagg tccaggagct ggtcctgttg
     3841 gagttaaaaa agaattagta ccttttttac caataccagt aattgaaaga aaagaagata
     3901 agtatgtact aaattatgat agagaaaaat ctattgggaa aattaaaaat ttctatggta
     3961 attttggagt tcttgtaaga gcttacacat atatactaac tatgggaagg gatggactta
     4021 aagaggctag tgaaatggca gttttaaatg ctaattatat aaaggaaagt ataaaagatg
     4081 attatatttt gccaatagat accttgtgta agcatgagtt tgtattaggt ggattgccaa
     4141 gaggagaggc caatataaaa actatcgata ttgcaaaaag actactagat tatggatacc
     4201 atcctccaac aatgtacttt cctcttatta taaatgaggc acttatgata gaacctacag
     4261 aaagtgagag tatagagaca ttagatagtt ttatagaagc tatgaaaagt gttgctaaag
     4321 aggcaaagga agaaccagaa ctattgaaaa ctgctccaca taatactttg gtcaaaagag
     4381 tagatgatgc aagggcagta aagaaaccta tactaacatg gtcacaaaga taaatagtta
     4441 aaaagtagag atttaaagta taaagagaat taaatttgta agaaaaagct atctaactta
     4501 atctagttta gatagctttt ttgtatatta gtatgtatgg tattttctat ataaagtttt
     4561 aaattatatt agtgcttata tagaatattg atatgatgat tatgtataga tattaataag
     4621 aattgttgaa tttatcttgc cttaa
//
LOCUS       DBGJFU010000205         4592 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.209, whole genome shotgun sequence.
ACCESSION   DBGJFU010000205 DBGJFU010000000
VERSION     DBGJFU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.209"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            552..1859
                     /locus_tag="ACOIAS_003618"
     CDS             552..1859
                     /locus_tag="ACOIAS_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703286.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1945..2207
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2333..3136
                     /locus_tag="ACOIAS_003619"
     CDS             2333..3136
                     /locus_tag="ACOIAS_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGZ2703287.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3174..3785
                     /locus_tag="ACOIAS_003620"
     CDS             3174..3785
                     /locus_tag="ACOIAS_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGZ2703288.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3817..4488
                     /locus_tag="ACOIAS_003621"
     CDS             3817..4488
                     /locus_tag="ACOIAS_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703289.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1843 a          419 c          825 g         1505 t
ORIGIN      
        1 tataagtaaa atatattaac ataaaggtaa aattttgtat actaaaaggg aatatttaca
       61 gtttaatggc agttttaaaa taataaatta aagctgtcat ttttatttaa aaatttttag
      121 aaaaagtaca atagtgttaa tgtgtgaatt agataaggtg tatataatag tttagatgat
      181 atgatataaa atatgttgta aattaaatgt tttctttgac aatatataca caaggatgat
      241 aatatctata tgaataaaag atagtgtgta atttgataat atgtaattat aacattatga
      301 tataattaca ataaagtatg taataatctg tagaaatttc aagctagtta tctaagtttt
      361 cggataacta aatctaaacg gaatacataa tgtattaaat aaatgatgat actaaaatga
      421 tactattttt atagaaattt attgaggtat ataattaatg aaaataatat attaaattat
      481 ataaattaaa ttatataaat taaattatat aatatattga gttttacata aaagcaaagg
      541 tgggaataaa aatgaaagaa attgctgatg ctataacatc agagtattta agctatggta
      601 tcaatgaaga tattagtatc tatgaaggta gattttgcat atatttagat aaaaaatata
      661 gatgtaatgg gaaaatttat tataaaatga cacctccaat atctataagt tttaaagcag
      721 acataggttg tgttgaagag atagacaatg aagatgacaa tttagcttta gattatgata
      781 atgctattct tgaagttcat ggatataaaa ttatttctat aacaataaat actttaagtg
      841 aatttagtgt agaaggttat atcaatgatg actgcataaa atctaagaac tcctatgttg
      901 aatatgtaga ttttaatata ataaacttag ataaaatacc tggaaaactc attaaataca
      961 atgataaggt atacgcaggt agaattgaat ttgatataaa tgattatgtt gtaactatag
     1021 acaagcgata tgattataga aaagaattaa aatcagaatt aaagtctaaa tctggagcaa
     1081 taataactca tataggtagg attagaagaa aagatggtag aatttttaga acaaataata
     1141 caataaatct tttagatagg atatcaacag ctttgagttt tatgtgtgga agatatgtag
     1201 ggttttgttt ggccaaaggg tatagaagtg gtaatgaagt ttatagaata tggaatgaaa
     1261 atcaaatatc tccttttaga tatgtaccaa catggtcaga tacactttca aattattata
     1321 atatggaaaa atatataagc cttatgtgta aaaagctaga agatttttat tatggttctg
     1381 ctataaagag tgttgttgat tggtacatag aatctcttgg aagtgctacc atggaaaaca
     1441 atataatatc tgtacaaatt gcacttgaaa ctttgtcata tgtaattcta gtggaacaaa
     1501 ataagatact tacagatgaa gtatttgact gtaatttggc atctaaaaat ataagactct
     1561 tacttgatac atgtaagata ccatatggta aacatgaatt aaatatattt gataatataa
     1621 taaaaaataa atttgatgat ggagtggatt tagttattta tttaagaaat agcatagttc
     1681 atccaagtag aaagactcat agagctgtgt tagaggttga agatatttgg aatataattt
     1741 caataggaac gagatatatt gaacttgttt tactctttat tttaggttat agaggagaat
     1801 attcaaacag attagtagaa agatgttatg gtgaagtgga agttgtacct tggaactaga
     1861 tttttatatt tattttaaaa tatatattga catatattgc cttatgctat aaaatcaata
     1921 tcataataat aattcagaat aacattaaat gctaagaaaa gaaatagtaa aagtagttaa
     1981 tcttaaacag agagtttccg attggtgaga ggagcaatag attttacttt gaacacatct
     2041 ttgagcagga ctctgaaaga gtagaaatac tttattagga gatttccgga atccttgcac
     2101 ccgttatagt gctagagtat aataatttta ttgtacttaa agaggttagt gtcgtgaggc
     2161 attaatgaac agaggtggaa ccacgagagt taactcccgt cctctagcag aagctagagg
     2221 cgggagtttt ttaatataat tttttaagcg cgcagagaat tatattgcaa tagatacact
     2281 ttttaaaaaa taatcaaaat attttaaaaa ttaaaaggcg agggagaata ttatgaaaaa
     2341 tatattaaaa aaagtaggaa tatttacaat tatgttaggt ttattaggtg gagttgtagg
     2401 ttgttcaaaa ccagataatg aaaaagataa ggatgcttca aaggaatcta aaaaagaagt
     2461 agttgtaggg tttgataata cgtttgtacc aatgggattt ttagatgaaa agggagatac
     2521 agtaggattt gacgtagact tagctaaaga aacatttaaa agacttggaa tggaagttaa
     2581 gtttcagcct atagattggt caatgaaaga aactgaatta aatgattcaa aaactgtaga
     2641 tgtattatgg aatggatatt ctataacaga cgaaagaaag aaaatagtat catatacaga
     2701 accatattta caaaataagc agataatagt tactctaagt gactcaaaaa taaattcaaa
     2761 agctgattta aaagataaag aagttggaac acaacaaggt tcaacagcac ttgatgcagt
     2821 ggaaaaggat aaagatttta tgaatagctt aaaaggtgga gcaccagttc tttatgatac
     2881 ttatgataaa gcacttagag atttagagat aggtagaact agtgcagttg ttggtgatga
     2941 agttttaatt agatactata tgggacaaaa gggtgaagac aaatacaagg tattaaaaga
     3001 cgattttgga ttagaagatt atgttgtagc aacttctaaa gaaaatccag aactttgtga
     3061 gaaaataaat gaaacattaa aagaaatgaa aaaagatgga acttttgaca agatttatga
     3121 taagtggttt aaatagaaga taagttagct gttaagaagg gggcactaaa ggcatgttac
     3181 aaggtttaga gatagttata gcaatgtttt gtataacatt gatagtatca attccattgg
     3241 ggattggagt tgcattttta agattatcaa agaataaatt agtaagtggt attacacaat
     3301 gttatatatt gataatgaga ggtacacctt tacttttgca aatgatagtc atattttacg
     3361 gattacctct tttaggaatt gtatttgaca gatttacagc tggagtagta gctttcttct
     3421 taaattatgc agcatatttt gcagaaatat ttagaggtgg aatacagtca atcgatagag
     3481 gacaatatga agcttcaaaa gtactaggat ttgataagtt tactatgtat aagagagtaa
     3541 tctttccaca agtttttaaa agaatactag cacctatttc aaatgaagta atcacattag
     3601 taaaagatac atctttagta tatatattag gattaaatga tattttaaga atatctcaaa
     3661 tagcaatgaa cagggaagca agtctgttac cattatttga ggcaggagcg atatatctta
     3721 tatttgtagc tatacttact aagggatttg aattacttga aaaaaaatat tcttattata
     3781 gataaagtta caatattata ggaggaggtt ttttatatgt tgaaaatcaa aaacttaaat
     3841 aaatcattta aaaaaaatag agtacttaaa gatatttctt ttgagttaga agaaggtcag
     3901 attggagttt tacttggtaa gtctggtgca ggaaagacca caatattaag gtgtataaat
     3961 ggtcttgaag aatttgatag tggagagatt ataatagata atgaggtaat aaaaaataag
     4021 agagatatgg caaaaatacg aggaaaaata ggtatggttt ttcaaaattt caacttattt
     4081 cctcatatga cagtccttga aaatataatt gaatccccag ttaatgtttt taaagttcct
     4141 agaaaagagg ctgaagagag ggctagagaa cttttaagat tagttgattt agaagataaa
     4201 ttaaattctt atccatttga attgtcagga ggacagcaac agagagttgc tatagctagg
     4261 tcatgtgcat tgatgccaaa agtcctttgt tttgatgaac ctacttctgc acttgatatt
     4321 gatactattc aaagagtagt caatataatg aacagactta aagacaaagg tatgacaata
     4381 ttaataatta cacatgatgt tgtcttctca aataatgttg cagataaaat tataagtata
     4441 aaagatggta tagtagaaaa tgttcaaata aaagaaaaaa ttgtttagag ttttataaat
     4501 ataaactagg gtagaatttt ataatataaa taaagggcaa gtaaaatcaa atatactatg
     4561 cccttttgtt atctcaaaat ttaatcttat at
//
LOCUS       DBGJFU010000206         4345 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.210, whole genome shotgun sequence.
ACCESSION   DBGJFU010000206 DBGJFU010000000
VERSION     DBGJFU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.210"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            247..936
                     /locus_tag="ACOIAS_003622"
     CDS             247..936
                     /locus_tag="ACOIAS_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2703290.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            936..1853
                     /locus_tag="ACOIAS_003623"
     CDS             936..1853
                     /locus_tag="ACOIAS_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGZ2703291.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRAIVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            1926..2852
                     /locus_tag="ACOIAS_003624"
     CDS             1926..2852
                     /locus_tag="ACOIAS_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703292.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            2849..3529
                     /locus_tag="ACOIAS_003625"
     CDS             2849..3529
                     /locus_tag="ACOIAS_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGZ2703293.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            3630..3785
                     /locus_tag="ACOIAS_003626"
                     /pseudo
     CDS             3630..3785
                     /locus_tag="ACOIAS_003626"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            3789..3962
                     /locus_tag="ACOIAS_003627"
     CDS             3789..3962
                     /locus_tag="ACOIAS_003627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703294.1"
                     /translation="MLKQMLENNLPIKIVSWDSEELYRNSLNSCSLGNYVPLIEYLSS
                     LEDFREVYKMLWK"
BASE COUNT         1640 a          461 c          706 g         1538 t
ORIGIN      
        1 aggtgtttta taatataaaa tagttaattg tagatgttaa taaaagaaaa ttatgtatat
       61 tttagataaa gataagagag tatactatat tttaattctt ttttcaataa agcttattga
      121 aaagaacttt aacttagaag agtatcacca aattgcagat acaatatcat aagcagttat
      181 ttattgacaa ccctttttaa taaatatatc atttgaagta tactgacgaa aaggagttga
      241 ataataatgg acatcttatt ggttgaagat gataagaata ttcgagaaat ggtatacgat
      301 tttctgatta gtgagaatta ctctgttaaa actgccattg atggaaatac agctattgaa
      361 atatttaaaa attattcatt tgatattatt ttactggact tgatgttacc aaagtctagt
      421 ggttttgaag tattaaaata tatacgaagt gtgagtgttg ttcctgttat tattactact
      481 gcgaaagata gtgacacaga taaaatgcta gggcttaatc taggtgcaga tgattatatt
      541 acaaagcctt tttcaatcgt agaattgctt gcaagaatta aagctaatat tagacgtgca
      601 acagtgtata gcagttcaag aaactctcaa gatatggtta ttcggtataa ggatttgatt
      661 ataaacatat caaaatatgt agtttcaaag aatggaattg acttaaactt aacacataca
      721 gaatttgaaa ttgttaaatt actagcgact aatcaaggac gtgctttttc aaaggaacaa
      781 ttatacaacg ctatttggaa agaaccttat tatggaaatg aaaatgtgct aaatactcat
      841 attaacaggt taagaaataa attgtctgat ggtatagaaa aaaataatta tattaaaact
      901 ctttggggaa ttggctacaa aatggaggaa gaataatgga gttattttta gttgtaattt
      961 cgattgtact atttgctctc tacttaaaag aacgaaataa atcaaagaaa atggaaagtg
     1021 attataagta tatcaattca agacttcgtg acataacaaa tgatgatgaa attaattata
     1081 ttttagtacc atctgatatt tcgattgtaa aagaaacagc agtagatata aataaacttg
     1141 ttgaaaagtt ttacagcagg caaattaatt ataatagaag tgaaaaagcg atattgcaaa
     1201 tatttacaaa catatcacac gatttacgaa ctcccatcac agtattaaag ggttatattg
     1261 aaatgctata tcttcaaagt caaaaagaag aattatcttc ttcaatgaga gcaattgtgg
     1321 aaaaaatgca aacaaattca aatgagcttg ttaattcaat aaataacctg tttaatatgg
     1381 caaaaatcca atctggagat atgatattaa atcttcaaaa atccaatcta acacaattat
     1441 gtcatgagat tattttagag ttttatgatt tgctagataa agaaaacttt aatgttgaag
     1501 ttaatattga agatagacct atatatgcaa atgttgacac agaagcatta caacgtatac
     1561 tcaaaaattt aattgataat gctataaaat atggttcaga tggtagattt ttagggattt
     1621 cactatatga aaaggaaaaa catgttttta tagatattga ggataaaggt tcaggtattt
     1681 ctgaaactga aaaagagcat attttcacaa gagcttatac tgctgacaga aaaaaaggca
     1741 atggtttagg tttggctatt tctcaagggc ttgcaaactc tatgggaggt tcaatttatg
     1801 taaatagtag acctaatgta aaaacagttt ttacggtaaa attgaaaagt tagaaaaaag
     1861 ttagatttaa aagagagaaa agacaatttt attttgtata ctgtttttag aaaggaggga
     1921 gtatgatgaa ctatatcata gagacccatc aattaaagaa aatatataaa gataaagctg
     1981 ttgttaatgc tgttaatatt catgtgaaaa agggagaaat ttatggattt gttggaccaa
     2041 acggggcagg taaaagtaca gtaatgaaaa tgttgctaaa tcttgttaat ccaaattctg
     2101 gagaaatagt tatgttcggt aagaaagtgg cagaaactga ttttgaaata ttaaaaaaga
     2161 ttggtactat tattgaaaat ccgtatttat atgagaattt gactgctaaa cagaatcttg
     2221 atttacactg tgaatatatg ggttattata acaaagaaca tatatcagag gtattggaat
     2281 gtgtcggatt gtcaaagcaa tcaaacaaga aagtttcaaa atattcttta gggatgaaac
     2341 aacgtcttgc aatagctaga gcaattctta ctaaaccaga attattgatt cttgatgaac
     2401 ctataaatgc actcgaccca gaggggataa gagaaatgcg agaattattc cgtaagttaa
     2461 atacagatta tggaataact atatttattt ctagtcatat tctttcagag gtagaacaaa
     2521 ttgcggacac tattggaatt attcaagatg gtaaacttat taaagaaatt tctatgtcag
     2581 atatacacaa atatcaaaca gattatgttg aagtagatgt agataatgtg gagcttgctg
     2641 gatacttgtt agaaaaagaa tttggaataa aagactttaa aattacttcg gaaagttgta
     2701 ttgaaatata tgatttacga aaagatgtaa aagaaatatc caaaatattt attcaaaatc
     2761 aaattggtat aaatagcatt ggacgtaaac aaagttcttt agaagattac ttcttccaaa
     2821 ctacaggtac aggaggtaaa gaaacactat gatacattta atgaaactag agctaaaaaa
     2881 agttgggcta aaaagatatt ttttattttc aatgctgggg attttcttat gtatgttttt
     2941 tgtctttact ggattgaatg ataaatctac ttcaatatat gattatgatg ttacatttag
     3001 gacaatcagt ttatttttct gtttttacta tattatatta tttgctgtgt tgaatgtggc
     3061 atatataata aatgaatata caaataaaac aatcttgatt ttattctctt atccacttga
     3121 ccgtaaaaaa cttattttat caaagttact tttgattacg actcttatga tatcatcaat
     3181 gatagttgga tatatctgtt gttttacatt cgttgttgtg ttggataagt attttaattt
     3241 agttattgga gattttcata tttcaatttt atcttattgg ataccaggag caattaaaag
     3301 tattattgta ttttgctcat taggtatatg gacattcgtt gttggaatga taagaaaatc
     3361 aatacctgtt acccttgtca gttcaattat ttttatcaat ttaagacaat ttattttagc
     3421 tggtacaaat acaactcaag acagtttgat ttctgttttt attatagttg gtataactgt
     3481 gattggtata tattatatac taaatcataa agttactgaa attgactaga tactgtggat
     3541 aggagggtag ttattaattg tatcttcttg ggataataat gatttgctat tatatcagct
     3601 attattaata ttattagagt tttaataata ttgcacaacc atttgaagtt gagccaaagc
     3661 ttgatgaatt aattgaatgg tactatagtc agagtgaagt atctattaaa gttatagctg
     3721 aatttcatta tagatttgaa ttgatacatc catttcaaga tggtaatggt cgcataggta
     3781 gatttgttat gctaaaacag atgttagaaa ataatcttcc tataaaaatt gtatcttggg
     3841 atagtgagga attgtataga aactccttaa attcttgtag tttaggtaat tatgtccctt
     3901 taattgaata tttatcaagt ttagaagatt ttagagaagt atataaaatg ttatggaaat
     3961 aattgtttgc agcaaaaaaa gtttttatat aaatatattt ttatctatgc tatttaacgc
     4021 taatttctta gttattctta gcttggacta tacccactat tttaactata taaaatagtg
     4081 gatatagttt aactatctct ttataactat ttttatgtat cttagttgtt tcttaatatg
     4141 gggacagttc ataaaactaa aagtgatttt taattttatc atttataatt taccattaca
     4201 tcagaaaaat gcttatcatt agaattttca taaaaggtac ctattttttt caattaactg
     4261 aatcaattct attagtctta atctttcata tttcaaattg ataatataag aaaataaaaa
     4321 ataaaaaatt ttaaattttt tattt
//
LOCUS       DBGJFU010000207         4291 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.211, whole genome shotgun sequence.
ACCESSION   DBGJFU010000207 DBGJFU010000000
VERSION     DBGJFU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.211"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..398)
                     /locus_tag="ACOIAS_003628"
     CDS             complement(<1..398)
                     /locus_tag="ACOIAS_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703295.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTL"
     gene            587..1048
                     /locus_tag="ACOIAS_003629"
     CDS             587..1048
                     /locus_tag="ACOIAS_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703296.1"
                     /translation="MLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKIDS
                     VFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKNNI
                     DISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            1091..2854
                     /locus_tag="ACOIAS_003630"
     CDS             1091..2854
                     /locus_tag="ACOIAS_003630"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HGZ2703297.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            2862..3470
                     /locus_tag="ACOIAS_003631"
     CDS             2862..3470
                     /locus_tag="ACOIAS_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGZ2703298.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            3542..4111
                     /gene="pduL"
                     /locus_tag="ACOIAS_003632"
     CDS             3542..4111
                     /gene="pduL"
                     /locus_tag="ACOIAS_003632"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HGZ2703299.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         1711 a          392 c          719 g         1469 t
ORIGIN      
        1 agagtaacta ttccttctat atctctagca gttaagtatt tatatggaat taataaatta
       61 taaccagtat tacttagtaa gttttttaat tgggttattt gagagatatt gaaacatttt
      121 gcagttttag tagatttttt tcttaagtta gataaatttt cattttgaat atttattaaa
      181 gtttctatat agtttctata agatatctta aagtttgtct cattgactat tttttcatta
      241 agtatactac tgataaaatc ttgagacaat atattatctg caaatttatc tgctgttaat
      301 tttccagatg ttatgccttt ttgatatttt ctaagaattt ttttagtata gcagtatatt
      361 ttatttctcg tttctaaatc gagttgttct atttgcacaa aaccacttcc ttctaattat
      421 aaaaatattt attaaaaatc tatacttaac taataaagct tcaataactt atttacaata
      481 aatactaaat cataaaatat ttgttatttt ttattattac caagtataaa aaatattata
      541 attgtaaaac ttacaaatat atgtaaaata tttaggagtg atgaaaatgt taaatgggat
      601 aataaagctt ttttattctg aaattgtaaa aacaaagatt aataacttag attcaagtgg
      661 atattcttta agagatgaat gtaaagaaat cagaaatata actctaaaga tagatagtgt
      721 attttttaag cttttaaagt tagcttttgg ttcaatattg cttatggtag cttttaattt
      781 aaacatggtt ttaggtatag gagtgttttt agttggtgta gtttatatac tatataaaag
      841 aaatatggaa gaacaagtaa aagagcatat aaataatgtg aaaaataata tcgacatctc
      901 taaaataact tttatggatg aaaaagggag aacaggtcta aatgcattaa ttacattact
      961 tcttatagga ttattgacag gatttaactg gatattagtt gctagttttg tcgttgtttt
     1021 tgtttttact ataaagaatg tatgttaaaa tactaataaa tagtttaata tataggggga
     1081 gaaattgagt atgactaatg gcagttataa aatgaagaat ttagtggaaa tatccgctat
     1141 ggtgactgaa tctacagatt tttttaatat aaaagatgac ataatagaaa aaatgttaga
     1201 agttgttcac cctacaaagg catgtgtgaa tctattttat aaaaatgatt ctaaatatgc
     1261 ctacttagta tgttcgcaaa ccttagaata tgtacctcaa atttttccaa taaatagtct
     1321 taaaggtgca aaaattgatt ttgacacata tccagagtat atacatgagg ctgtaaatga
     1381 aaaaaaaata atatatgtgg aaaatgtatt tgaagattct agagcagaag gtgaacgaga
     1441 tttagcaaaa gctgaaggtt atattgggag aatagttttt ccttttataa taaataacgt
     1501 agttgttgga tttatgactt gttttttaaa agaggatgat tatataacag aacaagatat
     1561 agattttata tcatctgttg catcactctt aagtttatca atagaaataa ctaataaaaa
     1621 taataatgta caaattttaa ttgataaact aagaggctca ataagtgcca taaatgaagc
     1681 tactaaaaaa ctatacctta ataagagtat tgatggattt ttggaaaatt taagtaaaca
     1741 agcgtgtaat attactaata gtaaagaagc tttaataatt ataaatagtc atcataataa
     1801 ggatgctata gttagttgtt ataatgttga gcaaaaaaag aaaacaaatt tatatcctat
     1861 gattgatatg tttttaaaga aagagtcatt gggggggtat ttaaataatt taaaaccatg
     1921 ctcaaaaaag ggaataaatt tggaaagtta tatatattac aaacttcagg ataaaaatga
     1981 gactatagga tgtatagttt gtgcaaatag caaaaactat actagtgatg atttaaatat
     2041 tcttagtata ttgtccaaac aagtttctgt tggtatgcaa ttatatgaac ataatgaaaa
     2101 ggaagtaaaa cacaaagttc ttgaaaacga gttgaacata ttaaataaac aacaacagct
     2161 tattatggat aaaggtaaaa tggattgtaa tgatgagaaa gaactttatt tttaccataa
     2221 accagcaagg gtagtaggtg gagattttta ttatgctaca aaagtggatg atgaacatat
     2281 agtttatatt gttgctgatg taatgggaca tggaatggta tcaaattatg ttgttgcaat
     2341 gataaaaggt gcttttaaag tattatgcaa tcaatacaat acaccaagag aaataatgac
     2401 aaatttaaat aggatgttat atgatgaatt tgacaagatg ggagtattta caacttgttt
     2461 gataggtatt atagatacta aagaaaatat gattactgtg tctaatgcag gtcactatag
     2521 tcctgtagtt gtaaagaaag atggcacaat agaaaataat ttaaattgta aaaagggcat
     2581 acctattgga ataatggaag atgctactta tgaaaataat actctttcaa tggaagatta
     2641 tgcaatggta tgtatgtaca ctgatggaat tttagagata aaaaattcat caaaagaaga
     2701 gtatggtata aacagattag aaaactttct aaaagaaaat tttagattaa atcaacagtt
     2761 aatagtagaa aatctaaaat tggatttaaa gaacttctca tcaaaagata attatgatga
     2821 tgatatttta atagttatgt taaaggatag gtagattgat tatggattca aacgataaaa
     2881 aaggacttaa aagtccagag attataataa taaatggaag tccaagaatt aataaaaatt
     2941 gcagttttat agcaaatgaa gtaaaagcta tgctagaaga gtgtggatta agttgtaaaa
     3001 tatttaatat ctacaatatg gatattgaat attgcacagc atgtggattt tgtgaaaaaa
     3061 cagggtattg caagtttaat gatgatatga agccattata taaaatgttt gatgattcaa
     3121 aaggaactat agtaataagt cctgtacatt ttgattcaat atcagctaaa ttaaagactg
     3181 ttgttgatag aactcaagct atatatgcaa gtaaatatgt attaaataaa ccttcaattg
     3241 atagaagtaa gaaacggata ggaatgtata ttgcagtggg agggagtaaa tcatatgatt
     3301 cacagtttaa aggtggacag attgttatgg attttttctt taagagtata aatactaaac
     3361 ttttacacaa tatatattta aacaatacag atgatgtatc ttatatagat aatcaagaat
     3421 ttaaagatat aatatataaa aatttaaaga tatatattga ttcaatataa tttgattttg
     3481 taggtaatat tatgtataat tgtattgcaa taatttaaat aattaaaagg agggaatttc
     3541 aatgaaatta ccaatagcat tatctaataa acatgttcat ttgagtcaag ctgatataga
     3601 cgtattattt ggagcaggtc atgagttaac taaatttaaa ccattatctc aaccagggca
     3661 atatgcctgt gaagaaaaag tggatttagt aggaccaaaa ggaactttaa aaggagttag
     3721 agtactagga ccagctagag ctcatactca agttgaaata tctttagcag atggtttccc
     3781 attaggagta aaagcaccaa taaaagaatc tggtgatata gctggaactc caggtgttaa
     3841 attagtagga cctgctggag aagttgaaat gaaagaagga gttatagttg cttctagaca
     3901 tatacatatg agcaatgaag atgctgctaa atttggtgta actgataagc aaatagtaaa
     3961 agttaaaaca tctggaccaa gagcattagt atttgaaaat gtattagtaa gagctagtga
     4021 cagctttaat ctagaaatgc atgttgatat ggaagaggga aatgcagcag gcgttaaaaa
     4081 tggagattta gtagaattaa tagttgatta atattataaa tagactttat gctagtagat
     4141 attttatttg ctagcatatt tttaaatgta ataaatttct ataggataaa tttaatcaat
     4201 tagattaatt tataaaataa aggggtatta taaaaaatgt aataaaaact tacaaaaata
     4261 tgatgtaata tggtaaaatt atatatataa a
//
LOCUS       DBGJFU010000208         4289 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.212, whole genome shotgun sequence.
ACCESSION   DBGJFU010000208 DBGJFU010000000
VERSION     DBGJFU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.212"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            231..1310
                     /locus_tag="ACOIAS_003633"
     CDS             231..1310
                     /locus_tag="ACOIAS_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HGZ2703300.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="ACOIAS_003634"
     CDS             1634..2002
                     /locus_tag="ACOIAS_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGZ2703301.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDNDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="ACOIAS_003635"
     CDS             2119..2922
                     /locus_tag="ACOIAS_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2703302.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="ACOIAS_003636"
     CDS             3307..4197
                     /locus_tag="ACOIAS_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGZ2703303.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1642 a          470 c          803 g         1374 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagaacgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tgctacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tctactaact
      901 ggattgtaaa gcatggttgt agaactctac taaaaaaagg aaatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagct atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggataa tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtttttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaacaa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaattaca tactcattag
     2941 gtaaattatg tagtatatgt gtttatagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggacagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagagat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaggcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat tgactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgtatgta tggtgtaaca
     3961 atgcgtagtg gtcagcgaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtatttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tgttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gatattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtatt
//
LOCUS       DBGJFU010000209         4285 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.213, whole genome shotgun sequence.
ACCESSION   DBGJFU010000209 DBGJFU010000000
VERSION     DBGJFU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.213"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(402..584)
                     /locus_tag="ACOIAS_003637"
     CDS             complement(402..584)
                     /locus_tag="ACOIAS_003637"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HGZ2703304.1"
                     /translation="MLNKKLPDTELKIMKCIWHTGYKTVISKDVADEIEKTYGWKHTT
                     TIILLARLTKKGFLIS"
     regulatory      810..907
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            962..1138
                     /locus_tag="ACOIAS_003638"
     CDS             962..1138
                     /locus_tag="ACOIAS_003638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703305.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1364..1564
                     /locus_tag="ACOIAS_003639"
     CDS             1364..1564
                     /locus_tag="ACOIAS_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGZ2703306.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            2315..2497
                     /locus_tag="ACOIAS_003640"
     CDS             2315..2497
                     /locus_tag="ACOIAS_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGZ2703307.1"
                     /translation="MFKFYNNATLMFLPGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2568..3413
                     /locus_tag="ACOIAS_003641"
     CDS             2568..3413
                     /locus_tag="ACOIAS_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HGZ2703308.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNKDPKYNLNTI
                     KKHLN"
     gene            complement(3637..3801)
                     /locus_tag="ACOIAS_003642"
     CDS             complement(3637..3801)
                     /locus_tag="ACOIAS_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703309.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1642 a          531 c          485 g         1627 t
ORIGIN      
        1 tctatagtgt tacttatatt ttatatatat ggctattttt caacatttta caatatataa
       61 catttatttt tttaactttt gtcagtaaaa tgtcagcaaa acactttatc cacaattttt
      121 atataagttt tccacaactt atcaccaata ttatccacat aaaaaaggta gcacttatat
      181 tattagctac cttttattta ttaatcttta tatttccagc tttttatcca acttcctatt
      241 tctattattt tttcttcagt tatttcttct ccatcatgta attttgaaat taattctgat
      301 aaaggattgt tatgtaaacc tctaaaaact ctttttccct ctattttgag atattctctc
      361 tcttttatta atactgtata gtgtacatgc ctcccaattc tctatgatat taaaaaacct
      421 tttttggtta atctagctaa aagtataatt gtagttgtat gcttccaacc atatgttttt
      481 tctatttcat ctgcaacatc ttttgatatt acagttttat aaccagtatg ccatatgcat
      541 ttcataattt ttaattctgt atctggtaat tttttattta gcataatata ttccttcttt
      601 caatttatgt cacttcttca aatttatcta gcttaaatta taataggagc tttttagaac
      661 aaaaactata tttaattttt accaggtact ctacgtgttt agtctgtttt taagtgtaaa
      721 ataatgttca tatctataaa tttatttaac tttcaaacaa tcctgttgaa taataactaa
      781 ttctattgta ttattttagt tgtagaataa aactaaatcg gcaaaactag agaaatttag
      841 tgacgcaaag ctatagggac taagacttat ataaatatct tatgagttat gtcagccagt
      901 tgccaaagag atagtgttct ttttgttttt atgaaagttt tttaggggga taataattta
      961 tatgtttgga aataaaatgg ataaatgtac acatgtattg actgcttata ttagcagttc
     1021 gtatgattat tgtaattttt tagatacaca gttagatgat tttatattag agtacggaga
     1081 aaatgtagta gagtcttgtt tacaccaagt aatggtattg gtaagtaagt ataattagat
     1141 agacattgat ttctagaaaa ccctttttag ttttatctac atttatatta atatatagca
     1201 atgtaaaacg tttaaatata tttttaatat taagaataat taaaatgtta actaaaaaat
     1261 ataattaagt agacatttct attaatctat gatacaatag tatttgtggt aatacacaat
     1321 taaatcaagt atatcttgat ataggttaat aggagtattt ttaatgaata atggaatagt
     1381 gaaatggttt aataatgaaa aaggattcgg ttttatatca atggaaggta gagatgatgt
     1441 gtttgctcat ttctcagcta tacaaacttc aggatttaaa tcattagaag aaggtcaaca
     1501 agtaagcttt gatatagtta aaggtgctag aggtcctcaa gcagaaaata taactatatt
     1561 ataatatata gatttttcgg gaaagactct ttttagagtc tttttttatt gttaatatat
     1621 gtaagataat cattggtgaa gtaaataagg tatattgagt aatgttcttg atagtttata
     1681 tgctaatgaa tttcaaaaaa ataagcactc ctataaaaag agtgctctta atgtaaatat
     1741 aaaccttcat tggtccagcc taataccccg tattctccaa gttttaactc ttttttatat
     1801 acattatcac tttcagccat atctaacaca atcatttctt ctttagaatc aatttgtaaa
     1861 atatgaggac tgtgtgttgt gacaattatt tgtgtttttg ggagaattgt ttttaatgta
     1921 ataaaaattt tacataccac atagttgata taaaacttaa gactagcaga ctcataagca
     1981 attagttgcc tctctttaca taccacatag ttgatataaa acctagaggc tctcagagaa
     2041 gttataagat gtttagtaac tttacatacc acatagttga tataaaactc caaaataaac
     2101 ttagcatctc caatgtttac acatatacaa tgctcttaaa attgcagtga acgcgcaata
     2161 ttgcaattga taatacttat catatattct caatacctta tatttcaact gctaaacctc
     2221 aatttatcac aaatatcgct cactgcaaaa tttctatatt tttattatat catagcatat
     2281 aattaatatt ttcaattaat ttttacattt aattatgttt aaattctata ataatgcaac
     2341 tttaatgttt cttcctggaa ctaatataaa aactatttca gaaagattag gtcatgcaga
     2401 tataaaaata actatgaata gctactctca gtttttagaa gagatggaca gagaagcatt
     2461 tgaaaatttg agcaaaatac tatttattgt attttaaaga agatgactta ttcaatattt
     2521 gatgctagaa tataattaat aaaaatttaa ttataggtga ttgtattatg aatattatta
     2581 aaagcataac tttactctta acattattaa ttgtgatatt aatgacaggc tgttccccaa
     2641 gagaaagtgc tagtgaagtt gcaaaaaaat atattgataa gatgaactct ggtgactatg
     2701 aaacagcata tagtttatta agtaaagata gtaaaaaaaa aattagtata gaaaatcttg
     2761 aagaatttca aaatatttta gttagtacaa aaagaataat tagttataaa ataggtaaag
     2821 aaaaaatcat aaaaaaatat aaacatgatg gaaaagaata taaaaatgtt gttaaattag
     2881 aagaaacttt taatataaaa aaccttcttt ataaaaaaga tgattctcta aaagtaaata
     2941 gatattttgt agttgaaaat aacaaataca agctattatt gtatgaaagt ttcaaaaaag
     3001 aatttggtat taatagtatg gaccttgctc aacttaagct taatgatttt ggactaaaat
     3061 actttaaaga agttgacctt atactaaaaa aagctattgc tattaatcct actgattcaa
     3121 agctttatta tgcccaagca tgtaattata tgaattttga tggatttcct ggttattcag
     3181 atacaacata taatgcttta gattcaatta ataaatcttt aaagtattta gataaaaatg
     3241 attctgacta caaaaaaaat gcaagtaata tatataactt aaaaggagtt attcttatgt
     3301 ttcacaaaag atttgatgaa gcacaacaat atttaaataa agccttatac tttaacaaag
     3361 ataataaaga cccaaaatat aatctaaata caataaaaaa acatctaaac tagatattta
     3421 atttttgaat ttataaaata tttttttatg tataattcag cactgaatca taatctctac
     3481 taaactaatt acaagtttaa attataaaga cttatttcta tataaatatt actctataac
     3541 aataagtttc ttatttaaat tttaaaatat tatctaaaaa tcataaaaaa gaaggtataa
     3601 agagctaccc tcaagctcaa tataccttat aaatatctaa acttcatttt ttactaattt
     3661 ttctatatct ttgataatat gttctttaga tttttttcca gctatggcat caaaaattgc
     3721 aacagccttt ttctctccaa acttttctgc tacatcatta aagtctaaaa atctaacact
     3781 ttcttttggt ttaccaaaca tataacacct ctcatacatt tttatatatt tattataact
     3841 tctatagact tatttttcac caataaacgt tagacaaatt agtcccttta tttttttaaa
     3901 ctagctttac cttcaaaatt cttaacaaaa acagtttatt ctactctata aataaaaata
     3961 gacaatttaa tttgtaggaa actgataata gaatacagta aataaaactt tgtttaatct
     4021 atctgaaaaa gttatctatt aaaactgcta tgaatttata aagaatattc tagttcataa
     4081 aaatacaaat atgtaaatat aatcacctta atattcaatt tatttaaaat aaaaaatgta
     4141 taaaagattt aataatgaca atatactatt ctcaatacaa tctatgatgt aaataattta
     4201 aaactactat attatttaaa aatttatatt tatatatgca attctaaaaa cttaataaga
     4261 agtgaactta gaaaataaag cttaa
//
LOCUS       DBGJFU010000210         4273 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.214, whole genome shotgun sequence.
ACCESSION   DBGJFU010000210 DBGJFU010000000
VERSION     DBGJFU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.214"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            479..1540
                     /locus_tag="ACOIAS_003643"
     CDS             479..1540
                     /locus_tag="ACOIAS_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HGZ2703310.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(1656..2630)
                     /locus_tag="ACOIAS_003644"
     CDS             complement(1656..2630)
                     /locus_tag="ACOIAS_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGZ2703311.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQILRDNNFYK"
     gene            3086..3967
                     /gene="pepI"
                     /locus_tag="ACOIAS_003645"
     CDS             3086..3967
                     /gene="pepI"
                     /locus_tag="ACOIAS_003645"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase"
                     /protein_id="HGZ2703312.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
BASE COUNT         1610 a          544 c          690 g         1429 t
ORIGIN      
        1 aaaattgatt ttttttaatc aaattttaag ttcaactcct tttttatatt tttatagata
       61 tatcctatat tttcccagaa aacgtattga atatacaaat tataagaagt ataatgttgt
      121 tgggagagaa tgtatgaaat gtatacaaag ttgattttaa ctgtattatt gataattctc
      181 tttactctta ccattattaa tgattgaaga caatggtatt tattataggt caaagattcc
      241 gaatgtatta atcatcatca ttttctcaaa ctaacagcaa ataccctgat ttgcgagttg
      301 tattgtatac aaattatcta gaaaatatct attagatttt ccataaaatt taaatatttc
      361 acgaatgtat gaatggagaa atatacatgt tgacaatcca cctttttaaa tactaaatta
      421 attttattaa tggtagagaa taggaagtaa caatgtttta taaaggagaa tattaagtat
      481 gaagaaaatt aggaagttaa ctaccaagtt attgattaca acaatagttt taatctcagg
      541 tatgagtatg acagtttatg gtatgacagc caaagaggta acagcaaaaa caccaaaaag
      601 ctatgtcact ggaaccaata gcgtatatgg tccaaagctg tcacaagccc aactaaactc
      661 agtagcacaa gcaacagcag attttatgaa caagaaaatc acaaagaata tgaccacaga
      721 tgctaagatt ttagttgcct acaatcacat taaaaataac acaacatatg ttgactggaa
      781 tgcagtagag ggagcaaata ctgcctatac attggttact aaaaaaggtg catgctcagg
      841 aatggctagg agcatgaaag ctttgtgtga tgctatggga attgaatctt attatgtaca
      901 cagcacatca aatgaccacc agtggaatct tatccgtttt ggtgatgggc gtttgtatca
      961 tgtagatatt gatgctaata aatcagcagg aaaggatatc atatacaaga gtcttagcca
     1021 cccattacca tttgataaga cagcatatcc aaaagttggt gcaatagtac agacggtaaa
     1081 accagaacag acacagaaac cagcaccacc tggaaagcac actatgttac cacatcaaga
     1141 aggggcaaag gctgttggaa attttggaaa aaaaggagaa gatataaatg aaaaacatgt
     1201 aatgtatgaa ggtgttagat gtagtgtagt taattcatca gtaaatacaa ttacatttac
     1261 aggtagtgaa tcaaatttag acccatcaaa tcttacgatt gctgtaatag gatttatgaa
     1321 cacaccatat gggatgtttg cattagcacc agagggtaaa cctgttgaat atccttttaa
     1381 atatgataag ccattttctt tagattttag tggtaaaaat cgttctggtg cagaattagc
     1441 gaagtactgt aaaaaaaatg atgtgactat taaaatagaa atcactgatg aaaaagctaa
     1501 tattggactt ggtcaaataa tgtatgttcg atatgaataa tgatttaatc atgacagtag
     1561 aataagttat aaacattgcg ccctcttact aggggcgcat ttaatattat atctgatatt
     1621 ttgtagtcag gtcaactgct agatttctcc cctttttatt tataaaaatt attatctcta
     1681 agtatctgag catgtttacg attaactaaa aactgtttta actcagtcaa gcataggaat
     1741 aaaatagctc tattttcaaa ctctgttctg tcaacaggca gaggctggtt tttcatacta
     1801 agcattagtc tctttaattc ttcaagaaga agattgcctg tttcagcttc aaattcagta
     1861 taacctatct tatgtataaa agcagataga atatgagctt gtggtggtgt agaatttagt
     1921 tgagatacat aactatacaa attttctaag atatcccgtt ggctttttat aatatccata
     1981 tgttctaaga agaatctagt atcacttaat aaagtattat taatattatc gtaagtttca
     2041 gaaatactgc tgtcaataag tgaatttaat gtatataaat ctctactata tccattttct
     2101 tttttaggat ttacaataat atctgcaatg tctataagta caatactcac ttcttcctgt
     2161 aacttttttt gaacttcatg gattttatgt atatttgttg gaatatacat atttattaag
     2221 acacctattc cagttcctac tgcaaatatt ccagcttcat ttaaaatcca tttgatagaa
     2281 gtttctcctt gaaggtagta atgggatgct atgactacac acatagcaat aacatcttga
     2341 atatttaaca aaaaacaaag tggtataatt ataaatataa atattgaaaa tgaagtcaaa
     2401 ttatatccta aaatattaaa acataaatat gaaaaaatag tacataaaag aaaaccataa
     2461 atctttccaa tggagccttt aagagtttct cttttagtgt cctttactgt aagtagtact
     2521 ataactccag caaccattga atattctaat tttaatagcc aagcaataaa tatagcaaga
     2581 gaacatccaa gagataattt tattacttta agtgaattaa tattattcaa atgcaaacct
     2641 cctgtaaatc aatataataa aattctaaca taaaactaat attgcttgaa aggtaagtaa
     2701 ctatacttaa ttattttatt gccctatttg tttagataca atcaatataa ttttatttta
     2761 tgaaaatttt ttgtaaaatt ttaaatttag agactaacat gagaccacaa agaggaagtt
     2821 tattataaga agaaaactta aaattgattt taaatagcaa atgaaaataa tcaaatatgt
     2881 atatactatt attaaaaaat aaaattatat agttaatata attttataat tggtttagta
     2941 tagagatttt taacttttat gttatactat ttataaaagt aggtataaag attaaaatac
     3001 ataatcaaat atgtataaga ggttttaaat agtattgtta atgaacttga ttattgttta
     3061 taattacaaa ttggaggaga taaacatgaa aattactgaa ggatatatgc cttttaaagg
     3121 ttttaaaacg tattaccgta tagtaggaga aaatacggaa gggaagaagc cattagtatt
     3181 acttcatgga ggtccaggtt ctacacacaa ctattttgag gtattggata agatagctga
     3241 aagtggtaga caagtaataa tgtatgacca aattggttgt ggaaattcat ttgtagaagg
     3301 gcatcctgag ctatttaatg cagatacttg gatagaggag cttattgaac taagaaaaca
     3361 tctaggactt gatgaaattc atctgttagg tcaatcttgg ggaggtatgc aggctatttg
     3421 gtatgctatt gaatataaac caaaaggtat taagtcttac atcctttcat ctactctttc
     3481 ttctgcaaaa ctttgggaga aagaacaaaa aagaagaata tcatatatga gtgaggttga
     3541 ccaaaaagca ttacttgatg cagtaaatac tggagattac tctagcaaag aatataatga
     3601 tgcattagaa agatttatgg aaatgtattg tgcaggtgaa gtgacagaag attctccaga
     3661 gtgcctgaga agacctaaaa aatcaggttc tgaagcatat attgtaggat ggggacaaaa
     3721 tgaattttct cctacaggaa ctctttctgg atatgaattt acagatagat tacatgaaat
     3781 aaaagagcct tgtctagtaa ctagtggagc aatagattta tgttcaccat atattgcaaa
     3841 gactatgtat gatagaatac ctaatagtaa gtgggaatta tttgagtatt caagacatat
     3901 gccattcgtt gaagaaaatg agaaatatat aaaagtctta actgagtggt tgaatgcgaa
     3961 cgattaaaaa ctatataaac ttatataaaa aaatgagtgt ctcaaaatga actttttagt
     4021 tcattaggca ctctttttgc atgaaatcaa atatattttc attatttgaa cgataaaatg
     4081 aggcttatat ctactttggg aaagtaattt tttattacta gcgttagatt ctaatctaat
     4141 tgtatacgta ttgaaataat agaaatatat tggtgtttat aacttaattt tattgttttt
     4201 tagaaatttt tttatatatt ggtataatta ttctgtttaa atttgattaa aaagtatatt
     4261 aattataata aga
//
LOCUS       DBGJFU010000211         4230 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.215, whole genome shotgun sequence.
ACCESSION   DBGJFU010000211 DBGJFU010000000
VERSION     DBGJFU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.215"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            244..1647
                     /locus_tag="ACOIAS_003646"
     CDS             244..1647
                     /locus_tag="ACOIAS_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2703313.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1738..1978
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2021..2719
                     /locus_tag="ACOIAS_003647"
     CDS             2021..2719
                     /locus_tag="ACOIAS_003647"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGZ2703314.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2742..3743
                     /gene="trpS"
                     /locus_tag="ACOIAS_003648"
     CDS             2742..3743
                     /gene="trpS"
                     /locus_tag="ACOIAS_003648"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGZ2703315.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1598 a          462 c          745 g         1425 t
ORIGIN      
        1 ttttataata aaataatatt gaaacaattt taaaaattta gtatcattag aattaaagtg
       61 gtaaaacttt taaatttatc atttttaata agatttgatt tcaaagtatt taaggtttat
      121 tattaaaact gtttttgaat atgcaaatat tttgaaaaat atcagtttaa taataatgaa
      181 tctattttat taattttgaa attaaattta aaataattaa tagagagggg aaattaaatt
      241 aggatggaaa agaaaaaaat aggcctatgg agtttggtat ttatgaatgt atcagcttta
      301 tatggtataa gatggatagc taaatctaca gcatcaagct ttggtttagg attaggtgca
      361 atacctatgt ggttattatt ctcatttata tactttgtac caggagcttt aatttgtgct
      421 gaattagctt ctacatatca aaaaggtgat ggaggtcttt acgattgggt taaggaagcc
      481 tttggagaaa agtatggatt tttagtttct tggttaaact ggacagctaa aatattttgg
      541 tattcttcat ttttaacttt cttagcagta aatgtttctt atacaatagg aaatccagcc
      601 cttgctgata ataagatgtt tgtacttata ttatctttaa taatattctg ggcattatct
      661 cttatagcaa ctaaaggaat aagttttaca aaaatattta ctaatacagg tgcattaggt
      721 tcaacaatac caagtgctat acttataata ttttcatttg tagcagtatt tgtattgaaa
      781 aaacatgata tagcttctac atacacaata gcttcaatga ctccaaaact taatatggat
      841 gcatttgttg ctatatcagc tataatgttt ggtttagcag gagcagagac tgctgctaac
      901 ttcataacag aaatagataa gcctgaaaag aacttcccta aggcaatatt aatatcagct
      961 ggaatagtag cttcattata tgtattaggt tcaattgcaa taacaatgat aataccacca
     1021 gaccaaatta ctgcttctaa aggtgtatta gatgcacttt ctgctgtttg tgcgtcttta
     1081 ggaattggtt cttggttggt tcaattaata gcttttggta tagctttttc agtattagga
     1141 gcaatagttc tttacatagc atcaccaata aaaatgttat ttggtagtgt taaaaaagga
     1201 atatttccag atagtttagt agaggtaaat gaacacaaga taccttctaa agcagttata
     1261 cttcaagcaa taatagttac tataatacta ttggtgacta cattaatgcc ttctgttgat
     1321 gctatataca atgttcttgt aactatgaca gcattaactg cattattccc atatgtattg
     1381 ttatatgctt cttatattaa actaagaaaa gagagaccag atgaaattag accttatact
     1441 atggcaaaat caacaagtac ttgcctaggt ttagcaaaaa tggttttagt tgtaactgta
     1501 gtgggtatat tattatctgc agcaccagtt atgcctacat ttgctgaaaa tgttatatat
     1561 gagatagaga tgattggtgg aggtcttcta gttatattat ctggtctatg gttatggaat
     1621 agatatgaaa agaaaacagc taaataaaat gtattttaaa aatttctttt ggatattata
     1681 atatatagtt gacaatttat agtgtaagga atattataaa ataaagaaat taataaatta
     1741 aatcctgaga taagaaatag taaataaata tatttttgca cagagaatta aggatgctga
     1801 gaacttaata aaaagtattt attgaatggg tctttgagga gtaaaatgaa atgtaattta
     1861 agattacaga gtatttgata tcgtgtatgc acgttatagc atatattaag atatatatat
     1921 tcaattttga gtgtatataa atttaggtgg taccacggaa aatatatccg tcctattgat
     1981 ttaggacgga ttttttaata tagaaaattt aggaggaaaa atgaacgtaa taggaattat
     2041 aggtgcaatg gatgaagaag ttagtatttt agttgattta atggatatta gagagactat
     2101 aaaaaaagct agcttagaat tttataaggg aattttggaa ggtaagaatg tagtgttagt
     2161 aaagtgtggc ataggtaagg ttaactctgc tctttgtgcc cagatactaa taagcgaatt
     2221 taaggttgat gctattgtta atacaggtgt tgcaggagct ttaaacgaaa aacttgatgt
     2281 taatgatata gtaatatcaa ctgatgctat acagtatgat gtagacacta cagcatttgg
     2341 agaccctaag ggagttatac caagaatgaa gacttctgta tttaaagcag atgaaagact
     2401 tatagatgct gcttacaaat cttctgtgga agaagtaaag actcataaag tattaaaagg
     2461 aagagtagtt acaggagata aatttataaa ttctaaagaa ttaaaagaag aattagtaaa
     2521 tgattttggt ggatattgtg gagaaatgga aggtggagct atagctcatg tatgctattt
     2581 aaataatact ccatttgtaa taataagagc aatgtctgat aaggcagatg gaagtgctga
     2641 tgttacttat gatgtatttg tacatgatgc tgccaataac tctaaagaca tagttttaaa
     2701 tatgttaaaa tcaatataaa tatataagga ggtacaaaaa aatgagtgaa aaaaaagtta
     2761 tattaagtgg tgcacagcca tcaggcaaaa tgactttagg aaattattta ggagctataa
     2821 aaaactggac tgaacttcaa gacaattatg attgttatta tagtatagtt gatttgcatg
     2881 caataacagt acctcaagac cctaaagttt taagagccaa tactatagaa ttacttgctc
     2941 aatatatagc ttgtggatta gacccagaaa aaaatactat atttatacaa tcacatgtca
     3001 aagagcatgt tgaacttatg tgggtattga atactatgac ttatatgggt gaattaagta
     3061 gaatgactca attcaaagat aaatcacaaa agagtgaagc aaatctaaat gcaggtttat
     3121 tcacatatcc agttcttatg gctgctgaca tattgttgta tcaaactgac ctagttccag
     3181 ttggagatga ccaaaaacaa catttagaat tagctcgtga tttagcaaat aggtttaata
     3241 atagattttc acctactttt gtagttccag aaggatacta cccaaaaggt ggagcaagag
     3301 tgatgagttt gcaagagcca actaaaaaaa tgagtaaatc cgattcaaat gaaaatgcat
     3361 ttatactatt agcagatgat tcagattcta taaagagaaa gataaaaaga agtgttactg
     3421 attctttagg tgttgtaaaa tacaatgatg aacaaccagg tattaaaaac ctaattgata
     3481 tatattctaa tttaagtaaa aagagtgtag aagagattgt aaacatgtat gaaggaaagg
     3541 gttatggcat atttaaagaa gatgtggctg aagtagtatc agaagcttta agacctataa
     3601 gagaaaaata tgttgacctt cttaataata aagattattt agaaaaaata tatagtatgg
     3661 gagcagaaaa agccgaaaaa caagctagaa aaactcttag aaaagtttat aaaaaagttg
     3721 gatttataga gagaagatac taattattag ctatctttac ttgataaata aattttaaag
     3781 ttattaaaaa ggtatatgat atgaaagaga gagtctcaaa gtgaactttt gttcatgagg
     3841 cactcttttt tgtatgaaat aaaatatatt ttaatcattt caacaatgaa aaagaggatt
     3901 atctctattt tgagacagca tcttttgata ataaaaaaag atgagctttt gctcatctca
     3961 aaggggttag attattctta ctttggggaa agtagaataa aggtaaaagt taactgatgt
     4021 caactttgtc ttttttgcat atcactactg agcataaaaa aatcagaaaa ccatagcctt
     4081 ctgaaacaac ttctaacata cattgtattt tatacccaat atgtaattta tgagttaatt
     4141 ttaactctta taatgaaaat tgtcaataat atatcaaaaa aatatttttt aaatttatat
     4201 aattaaatga atttatgttt atatataggt
//
LOCUS       DBGJFU010000212         4191 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.216, whole genome shotgun sequence.
ACCESSION   DBGJFU010000212 DBGJFU010000000
VERSION     DBGJFU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.216"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            93..1787
                     /locus_tag="ACOIAS_003649"
     CDS             93..1787
                     /locus_tag="ACOIAS_003649"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HGZ2703316.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2119..2229
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2333..2704
                     /locus_tag="ACOIAS_003650"
     CDS             2333..2704
                     /locus_tag="ACOIAS_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HGZ2703317.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2801..3748
                     /gene="trxB"
                     /locus_tag="ACOIAS_003651"
     CDS             2801..3748
                     /gene="trxB"
                     /locus_tag="ACOIAS_003651"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGZ2703318.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3852..4169
                     /locus_tag="ACOIAS_003652"
     CDS             3852..4169
                     /locus_tag="ACOIAS_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HGZ2703319.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         1740 a          388 c          781 g         1282 t
ORIGIN      
        1 tatatataat aatttataaa agagtattac atagtacatg taaatctata tttataaata
       61 caataaagta aagttgtaaa ggagtgcttt aaatgaagtt ttcagagttt aagtatgaaa
      121 gacccaatta taatagtatg aaaaaagagt ttttgagttg tgtagaagat ataaataatt
      181 ctcgtaatta taaagagcaa caaaaaaata tacataagat taatttatta agaaataaaa
      241 tagaaacgtt gtcaaatata gcttcgataa gatatagtac agacacattt aataaattct
      301 acaaagaaga aaaaaattat tgggatgaat atatgccttt gtatgaagaa ttaaattcat
      361 atttttataa tgccattgta aattcaaaat ttaaatatga tttgataaaa gagtttggag
      421 agcaattttt cacaatagta gagtattctt taaaaagttt ttccaaagaa ataataagtg
      481 aattgcagga agaaaataaa ttgtgttcag agtatactag acttttagct tcagctgaga
      541 taatgtttga tgggaaaata agaaatttat ctggtatggg gaaattcatg tattctaaga
      601 atagaaaaac aagagaatta gcaaacaaag catattataa tttctttgaa gaaaatgaaa
      661 ctaaatttga tgacatattt gataaattgg ttaaacttag agataagatg tctaaaaaat
      721 taggttttga agattttgta gagcttgggt atgttagaat gatgagaagt gactacagag
      781 aatatatgat aaaaaatgtt agaaaacaag tactaaaata tgttgttcca atggcaaatg
      841 agctttatga aaaacaagct aaaagaattg gtctagaata tttaagttac atagatgaag
      901 gcgtggaatt tttaacagga aatgcaagtt taaagggaga ttcaagatac ataattaaaa
      961 atggtaaaag gatgtattct gaactgtcaa aagaaacaaa tgaatttttt gattttatgt
     1021 tagaaaatga acttatggat ttagaaacga agaaaggaaa aggagctgga gggtattgta
     1081 cttatatacc agattataag tcaccattca ttttttctaa ttttaatcaa actgctgatg
     1141 atatagatgt attaacacat gaggcaggac atgcatttca gttgtatatg tctagatgga
     1201 ttgatatgcc agaaattaat tttccaacgc ttgatagttg tgaaatccat tcaatgagta
     1261 tggaatttat aacatggccg tggatggatt tattttttaa agaagataca gataagtaca
     1321 aattcactca cttatcatct tcaattaaat ttatacctta tggagtaatt gttgatgaat
     1381 ttcaacacta tatttacaaa aatcctaatg tagataagtc aaaacgaaaa gaaatctgga
     1441 gatttttaga gaaaaaatac ttacctcata gaaaatatgg tgataattct tttttagaga
     1501 gaggttgttg gtggtttaaa caaggacata tatttaaaaa tccgttttat tatatagatt
     1561 atgtattagc tcaaatttgt gcgttacaat tttggaaaaa aatgattcaa gatagagatg
     1621 caggatggaa agattatata aatatctgta aagttggagg aacaaaatca tttttagata
     1681 ttgtgagtat gggaaatttg tattctccat ttgatgatgg ctgtattgag tctattatag
     1741 gtgatgttaa atcttggttt gatgaaataa atgacagcaa attataaaaa attatgagaa
     1801 ttttattgta taaaagtgaa aaattaaaaa atatataaaa agtgattatt tgttgtgaaa
     1861 atagtcttgt ttttaactaa attgataaaa taaactaaaa aagatttgta cttaaaaaaa
     1921 aaagaaaata caagaaatat gcaatatcaa catttggaaa aaaaagtaaa aatgtataca
     1981 aaatacatat aaaaaaacta ttgcaaattt tatagaatgt ttatatactt aagtcaacag
     2041 agatagaaaa aagctattta tagaaaacaa aggataaatt gcttaatttt aaattttctg
     2101 aaaaaaataa taatccggat gaagagtatg ggagactgct ttataaaaag cgaccgaagg
     2161 gacaagttta atcttactta cccaaaagat tagatgaaat tctcaggtaa aggtgactgt
     2221 acttggacgg ggctctgaaa atgattttaa cagggaagac aaattgggag atttgttttt
     2281 cctgtttttt atttatcaag taataaataa aagtttgaaa gagagtaaaa aaatgataat
     2341 aattacgaat aatccaaaag taaaagaaga agttcaaggc agagaagttt tgtttaaaga
     2401 cacaacttat attggaatct tagaagcaag tagagatttg atacatgaag gttatgaact
     2461 tttatctcat ccactctatg ggagtgtaaa gccaaatgaa acaccttaca ggactgttat
     2521 actaaagaaa ggaaatcgtt tggatataaa ctctttaacc ttaattgaag aagcaatcat
     2581 cactgcaagt aaatttcaga ataataaaaa gactccaaaa tggacagaaa gtgttcaaga
     2641 tgattttaga gtaattgatt atgatatatt ttataataca atacaaagaa tgcagtatga
     2701 ataaaaaaaa attgttaaaa agttattaaa acagcctaaa tattaaatct aatttaatat
     2761 taaataaaat gagaaattta attggggagg ataagtcaaa atggaaaatg tgtatgactt
     2821 agtaataatt ggttcaggac cagcagggct tgcagcaggt ctttatggag caagagcaaa
     2881 gttaaaaact ctaatcctag aaaaagacaa gactggtgga caaattgtaa taacacatga
     2941 aattgcaaat tatccagggt cagttccaaa tgcaacagga ccaagtttaa tagctagaat
     3001 ggttgaacag tgtaaagagt ttggagcaga aatgctaaga gacaacattg ttgatactga
     3061 gctagatgga gatataaaag ttttaaaagg ggaaaaggca gaatatagag caaaagcagt
     3121 aataatagga acaggagcaa caccaagaaa gattggttgt cctggagaaa aagaacttac
     3181 tggtaaaggg gtttcatatt gtgcaacttg tgatgcagat ttctttgaag attttgaagt
     3241 atttgttgta ggtggaggag acagtgcatt agaagaagca atgtatttaa ctaaatttgc
     3301 tagaaaagta acaattgtac atagaagaca aggatttaga tgtgctaaga gtgttgaaga
     3361 aaaagcaaaa gctaatccaa agatagagtt tttattagat acagtaattg aagaaatcaa
     3421 aggtgatgga atattagaat cagttgtatt taaaaataaa gttactggag aaactcatga
     3481 atactttgca gatgaagaag atggaacaat gggagtattt gtgttcgttg gattagatgc
     3541 tcaaactgat ttattcaaag gtaaagttga tatggatgaa aaaggttaca taataactga
     3601 tgaagatatg agaactaaca taccaggcgt atttgcagca ggagattgta gatctaagac
     3661 tctaagacag gttgtaactg caactaatga tggagcaatt gcatctatag tagctgagaa
     3721 atacattgat gaaaaatttg ggaactagaa tctgtaaatt aagaaatagt gtctgtaaat
     3781 ttaatgaata atatttaaaa ttaaaattaa tatttaaaat taaaataaaa atttcaaggg
     3841 ggaataagaa aatgttagat ttagataagg ctacatttga agaagaagtt ttaaatgcag
     3901 aaggttttgt atttgttgat ttttggagtg aaggttgtga gccttgtaaa gctttaatgc
     3961 cagatgttca taaattagct gagacttatg gggataaaat aaaattctgt aagatggata
     4021 caacaaaagc tagaagatta gctataaaac aaaaagtatt aggtcttcca actatggcta
     4081 tatacaaaga tggagaaaaa gtagacgaag ttactaagga tgatgctaca gttcctaata
     4141 tagaaaacat gattaaaaaa tatctttaat atgttgataa attattaaca t
//
LOCUS       DBGJFU010000213         4136 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.217, whole genome shotgun sequence.
ACCESSION   DBGJFU010000213 DBGJFU010000000
VERSION     DBGJFU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.217"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            34..432
                     /locus_tag="ACOIAS_003653"
     CDS             34..432
                     /locus_tag="ACOIAS_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2703320.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIK
                     INLNIKGYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            420..1358
                     /locus_tag="ACOIAS_003654"
     CDS             420..1358
                     /locus_tag="ACOIAS_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HGZ2703321.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1742..1966)
                     /gene="secG"
                     /locus_tag="ACOIAS_003655"
     CDS             complement(1742..1966)
                     /gene="secG"
                     /locus_tag="ACOIAS_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGZ2703322.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2559..3851)
                     /gene="eno"
                     /locus_tag="ACOIAS_003656"
     CDS             complement(2559..3851)
                     /gene="eno"
                     /locus_tag="ACOIAS_003656"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGZ2703323.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1432 a          661 c          462 g         1581 t
ORIGIN      
        1 ttaataactt atataaaatc ttaaggagtt gccatgataa aaaaaataga acatgtggtc
       61 attactaata ataatataga aaattatatc aatttttata aaaaatttgg tttttcatct
      121 aaagaaatga gcaaaaatta taaacttaat gctagaaata ttaagataaa tcttaacata
      181 aaaggttata aatttttacc ttatgttaaa aatttacaag caagtagaga taacttatgt
      241 tttaaaataa acacaaatat tgatgagcca aaaaaatact tagaacaaaa tgagattcaa
      301 attaagcgag gaattgtaga atgtacaggt gtctttggta atatgaaatc tatttatttg
      361 aaaaaaacta atggaaattt aattgaatta tcaaatcata gaatcacgga gagtaaacct
      421 tggaaaattt aatcctatca ataaatgttg ttcttccttt gtttctcaca atgtcacttg
      481 gatatgttct aaaaaaatta ggtctatttg atactcatac tttaaataaa atgaataatg
      541 tctgcttcaa gtctttcttg ccattattat tattttttaa tatatacaat acagatttag
      601 atggttctct taattttagg cttatggctg tagctgttat aattattttt tcaatcttct
      661 taatcttagt ctttattatt cctaaaattg aaaaagataa taagacaagg ggtgcattaa
      721 tacaaggtgt atttcgtagc aatttcgtta tatttggtat tcctgtgaca atttcactat
      781 ttggcgataa gagtattgga gtcacatctt tattaattgc agttattgta cctatgttta
      841 atgtattatc tgttgtaatt cttgaaattt ttagaggtgg caaagtaaat ataaaacata
      901 tgattaaaag cgtcttaaca aatccactca taatcgcctc ttttatagga ttgacaatgc
      961 taattttaaa tattaatata cctacatttt tacacaaaac tattggtgat atttctaaaa
     1021 tagctactcc tttatcactt ataattctag gtggctcttt tagtttttct gctataaagg
     1081 ggcatataaa ccccattata ttgggcgtat gtggaaaact tatacttgta cctgctatat
     1141 ttttaccaat tagcattttt cttggattta gaaatgtaga attagcttca cttatgataa
     1201 tgttatctgc tccaactgca gtttcttcat ttacaatggc acaacagatg gattctgaca
     1261 gtgaattagc tggacaaatt gtagtattta cttctggatt ttctataatt acagtatttt
     1321 taataatatt tattttaaag cagaataatc ttatttaatg atatttgata acacaaattg
     1381 gtattatgag aaacatatat aaaatcgatt taattgcgta aaaacatatt attatactat
     1441 ttttactata taatataact ttgtgtatat tatctcatta aacatggtga aagtagtgtt
     1501 gctatatatt ttttagtttg aaaaaaatat ataaatacaa acaatatata aatacaaata
     1561 ataaacgcca acaaatagtc aatctataaa ataaaaactg gactaatcat ataaaatcat
     1621 tagtccagct tttattttac gtacttttta tgttgatgta cttttattaa aaactaaatt
     1681 taatttttaa atgtctagca aatgcactaa ttaaataatg gccagttatt caacaatatc
     1741 actacttctt aactataagt aatgccaatg catttataaa aaataatact gctgttatct
     1801 ttgtaactct agcaagaaat gcttgttttc cttttggttt aaagtaagct tggcttcctt
     1861 ctccaccaaa ttctgaaggt aaagcattct tagaatcttg aggcattatg ctaattacta
     1921 gtaaaatccc tagtactact tgcacaccca ttaaaatgtt gctcattctt gcccctccta
     1981 tctgtttatt acttaaattt cattactctt tagtgtatca caataatata gccatttcaa
     2041 gactattatg ctataaataa catacaaaaa aatatattgt tactataata tattactata
     2101 ttctcaatat aaaaatgaat aaaacttaga agagaaaact atttaaatta atgcataaat
     2161 taacacttaa aataatctac tctcattgta tatattataa atagccacca aaataaaaca
     2221 atcattttaa aatattgata taacttttaa tttcaacata atttaaaata aataatccac
     2281 atttagcaat aatactttaa tgacaattat gattaaatca aaatatgtct tcacttttat
     2341 tttttaataa aaacctccta tgcaattaga taaaaatgag atagaaacat ttattcatat
     2401 tttttctgaa tctatgaata aaattaagta ataaaatata aaaaaggata agtgaattga
     2461 aaaatcaatt aacacttatc tcttttatat cttataagat gagcttaaat atcttatatt
     2521 ctctcttcat taatcttttt tagtaaccat tttaataact atttttttaa gttatagaaa
     2581 gattttaatc cacaatatct agcttgctct ccaaccattt cttctattct taataattgg
     2641 ttgtacttag caactctatc agttcttgat ggagcaccag tttttatttg accagcattt
     2701 actgcaacag ctaaatctgc tatagtagaa tcttcagttt ctcctgatct gtgagatata
     2761 acagcagtat atcctgctct cttagccatt tctatagcat ctaaagtttc agttattgta
     2821 cctatttgat taactttaac taatatagag ttagctactc cattttcaat tcctttttct
     2881 aatctctctg tgttagtaac aaataagtcg tcaccaacta attgtaattt atttcctaat
     2941 ttttcagtta ataatttcca tccatcccaa tcttcttcgt ctaaaccatc ttctatagtt
     3001 attattggga agttgtttga ccaatcttca tataaagcaa ccatttcagc agcagttaac
     3061 tcttttcctt ctcctgctaa aacgtatttt ttagtttctt tgttgtacat ttctgtagca
     3121 gcaacgtcaa gacctaacat aacgtcttca cctggcttat atccagcttt tgttatagct
     3181 tcaactatta attctaaagc ttctctgttt gaacctaagt ttggagcaaa tccaccttcg
     3241 tcacctacac cacaagctaa tcctttttct cctaaaactt tctttaacga atggaatact
     3301 tctgctccca ttcttaaacc ttctttgaaa caacaagctc ctactggtaa tatcataaat
     3361 tcttgaacat caacgttgtt atcagcatgc tctccaccgt ttaatatgtt catcattgga
     3421 acaggtaatt gtttagcatt tactccacct aggtattgga ataatggtaa acctatttca
     3481 tcagcagctg ctctagctac tgccattgaa acacctaata tagcattagc tcctaattta
     3541 cctttgtttg gagttccatc taattctatc atgattgcat ctatagctgg ttggtcaaat
     3601 gcatccattc cttctatttc aggtgctatt atttcattta cgttagcaac agctgtttct
     3661 acaccttttc ctaagtatct tcccttatct ccatctctta attcaacagc ttcaaaagct
     3721 cctgtagatg ctcctgatgg aactattgct cttcccattg ctccatcttc taatactact
     3781 tcaacttcta cagttggatt tcctcttgag tctaatactt ctctagcata tactaattca
     3841 ataactgaca tttgtatatc tcccttcgat tttctattag ttaaattttc atttactgta
     3901 aacatccgtc tactatttta atctactttc tatattatag tacataaagt tgcttttgaa
     3961 aattaggtac aaatttattt gatgggtaaa tttgtaaaag ttcctatttt tattttattt
     4021 tttataatat tttaatttat ataatataaa aaaacttgtc taaaatccaa aaccaatctt
     4081 aaatacacaa atattataac tagatttaaa acattaaaca taaaatgtac aactaa
//
LOCUS       DBGJFU010000214         4035 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.218, whole genome shotgun sequence.
ACCESSION   DBGJFU010000214 DBGJFU010000000
VERSION     DBGJFU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.218"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(216..1466)
                     /locus_tag="ACOIAS_003657"
     CDS             complement(216..1466)
                     /locus_tag="ACOIAS_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HGZ2703324.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1499..2440)
                     /locus_tag="ACOIAS_003658"
     CDS             complement(1499..2440)
                     /locus_tag="ACOIAS_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HGZ2703325.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2680..3756)
                     /locus_tag="ACOIAS_003659"
     CDS             complement(2680..3756)
                     /locus_tag="ACOIAS_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HGZ2703326.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT         1215 a          732 c          427 g         1661 t
ORIGIN      
        1 taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag
       61 aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac tattattaag
      121 aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa acaagggcta
      181 aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc atgtaagaat
      241 agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat cttatttgca
      301 tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc tactttcata
      361 tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat aaaatcaatt
      421 cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata gtcttgtaat
      481 atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt atttgctttc
      541 attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc aggtttatat
      601 ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt tataaattct
      661 tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac cattaaattc
      721 tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt ataatttgga
      781 tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg atgaccatct
      841 gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat tatatcatca
      901 ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc atataaaggt
      961 tcattattat ccataaaatc attgattata tcatctatca ccctttgatg tttgtaagtt
     1021 acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct atcaatttcc
     1081 ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc atcaactgat
     1141 atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata tatatatata
     1201 cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa attttctcgc
     1261 gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc aatttctgat
     1321 ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc ttctttacta
     1381 ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt tggtctaatc
     1441 gccttaaatg gtttaatcgt tgccattact aatttcctcc ctaattatac aaaatatttt
     1501 ataatgctac ccctattaga ttgttgaagt caagaaagaa atcttgaatc acatttacta
     1561 tttcgtctcc tattctttct tgagcttcta ctgtagatgc cccaatatga ggtgtaggag
     1621 aaactcttgg atgatttact aagtctaaat ttaatgtagg ttcttgttca taaacatcta
     1681 tagcagctgc tgataattta ccttcatcta atgcttttag taaatctttt tcatcaacta
     1741 aaccgcctct tgcacagttt attaaataag cccctttctt catcttattt atttcttttt
     1801 cagttattac tgccccctta ttcttatcaa atggtatgtg taaagataag aaatctgcat
     1861 tttctaaaac ttcttccata tcacagaatt tataattgtt aaagccttgc gcctcaccca
     1921 ttatgtcagt atatataaca ttcataccta ataattctgc tctctttgca acttcttttg
     1981 caattcttcc aaaacctata agtccaagtg tctttccatt aatttctgta cctttatact
     2041 ttttcttctc ccattttcca tctctcatag ttacatttgc agtatttatg aatcttgcaa
     2101 gaacaaataa ttgacctatt gtaagctctg caacagatat actgcttgca tttggagtat
     2161 tcataacttt tatgccatta gcttgagcat atttaacatc tatattatcc agacctactc
     2221 cacctcttac tatcaacttc aactttttac cctctaatgc tttatctatt acatctttag
     2281 taactttagt agctgaacgt actacaagta catcaacatc tttaagttta tctcctaaaa
     2341 catctttttc ataaaactgc tctatcacat taaaatctaa ctctctcaat tttctcgctg
     2401 cttctttctc tattccatca gtaactaata tattatacat aacaaactcc tccaattttt
     2461 attcacttaa tttttccaat ttttattttt taaagtattt tcatcaataa aaaactttac
     2521 ttaatttaac tttctgttta tataattttc aaaatatttt tttattaaag tcatatcaaa
     2581 ttttcctatt gtttgttaaa aatttaatat gtaattttta taaaatatta tttattatat
     2641 attctaaatt tatcttaaaa catacatatt tttacaatat tatttattaa aacctaatat
     2701 atcatcaata ttatctaata attcttgtac atcttcgaca gtatagtcac ccatatgtga
     2761 aattctaaaa gttttttctt ttaaatctcc atagccattt gctatttgga atcctcgttc
     2821 ttgtaatttt gaatttaaat ttgatacact aataccttga gtattttcta taacagtaag
     2881 tgtattggat aagtgatttt cattggtaaa aatttggaag tgttttttag cccagtttct
     2941 aactaagttt gccatgtctt catgtctgtt gaatctgtta tctaggcctt catctaatat
     3001 attatctaat tggaaatcta aagcgaacat gtgagaaaga gatggtgttg aagggtattg
     3061 gtagtttttc ttgattaagt attcatacat tgctaacaaa tcaagatata ctcctctaaa
     3121 aggtacttgc ttagctctat cgattgcttt ttgagaaaat gtgcaaatag acattcctgg
     3181 tggtaatccc agtgcttttt gtactgatgt tatacaaata tctattccaa tcttatcaac
     3241 ctcgactttt attccaccgg ctgaacttac agtatctaca cagtagatta catcttcata
     3301 ctttttaaca acttctccaa tttcttctat tggatttctt attccagttg aagtttcatt
     3361 gtgagttaca gttattaaat cgtattctcc ggtagaaagc accttatcaa ccatttctgg
     3421 cgttgttgcc tctcctaatt caactttaaa tatatccgct gggacattat tagccactgc
     3481 cattttgtac catctatcac caaaggatcc acaggaaaat actgctgcct ttttggatgt
     3541 acaggaacga atggagcctt ccattaaacc agttccggat gacgtggata aaagtatcgt
     3601 gttgtttgta tagaatagtt tttgcatctt ttcacttata ctcttttgga gcatagatgc
     3661 atcttttcct ctatgtccta tcattggtgt agccatcttt tgcaagactt cttctttaac
     3721 atcaatagga ccaggaataa ataatttctt actcattata attcccccct ttttagtttg
     3781 taatatggat agcgttttta ctgtccttat ttttaataac tataatatca agctgaatta
     3841 actatgtcaa cataaaattt attttttata tataatttat tttatatatt ttatgaaaac
     3901 attcattttc taccttttca taagtctgaa ttttctgaat tattctctca aaaaaaatat
     3961 actttttatc tatataaatt aattttttta gtatgtaaat ttgacgctat tacgataaat
     4021 ctttttagta taact
//
LOCUS       DBGJFU010000215         4028 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.219, whole genome shotgun sequence.
ACCESSION   DBGJFU010000215 DBGJFU010000000
VERSION     DBGJFU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.219"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(199..735)
                     /locus_tag="ACOIAS_003660"
     CDS             complement(199..735)
                     /locus_tag="ACOIAS_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGZ2703327.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(753..2066)
                     /locus_tag="ACOIAS_003661"
     CDS             complement(753..2066)
                     /locus_tag="ACOIAS_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HGZ2703328.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2421..2861
                     /locus_tag="ACOIAS_003662"
     CDS             2421..2861
                     /locus_tag="ACOIAS_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HGZ2703329.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3046..3234)
                     /locus_tag="ACOIAS_003663"
     CDS             complement(3046..3234)
                     /locus_tag="ACOIAS_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703330.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT         1418 a          670 c          386 g         1554 t
ORIGIN      
        1 ataaataaac ttgtattaaa atacatttag ttatataatt ttattttttc taagagtata
       61 attataaaaa taaaaaaaga ataggataat tttcctactc ttttttctgt acttacatat
      121 aatattaaaa ctctttattt tttaatatat attttaaatt ttctttttta ataaatcttt
      181 ttcttttaat aaatctattt aattttcttc ttctatatca gagccaaagg gaatccatgc
      241 attaccaaac tctttatctt taaataaata agctaatcca gttatttgct tcaatctaag
      301 aatttcatca acttccatac ccaggtgctt tgatatccat tcatcagatt ttccaatttc
      361 acaaagttca gttataattt tactcattaa atccacatta tgagaacctc ttgcacgatt
      421 gtgtcgaata gtacttgcca ttctttcatc aagagattta tcaattactg aaacaggaag
      481 aagtccattt tctctatcat atatatcctc attttcaagc attactctat atctatgaaa
      541 accatctaca ataatatact tatccttttc ctcattataa taacagacaa ttggcattgt
      601 atacccatct tcttttatac ttttatacaa caactgcatt tctggtgggg ctactgcatt
      661 tggattatag tcgttagatt ctatcttttc gattggcact gcgatgattc catatacagg
      721 acttttatat tccatagcat cttaactcct atctaatgaa ttttttcata tttcttctta
      781 atatattccg ttctaattct ctgttctttt gtaggaccaa atcccataaa tctacacata
      841 tggtcatttt ttaaaataca ataacacatt cttttccaac ttggtatatc tttggtaaat
      901 ttaatatcat ccgtatcatc aggtatatca tccaaaaata tgactctact ctttttatct
      961 tttgtatgat ttgaaactcc atttaattct attttataac cttttctcaa caattcacgt
     1021 ataactattt ctgacaaacc accacctgta ttctgccaaa actcaataga tgttgaaaat
     1081 tttttaatat agctattcct tatattttca ggtaaggtat caagcaaaaa atatgtgaaa
     1141 cttttccatg tgtgtccata tggcaattta acactacgat atcccatagc ttttgttttg
     1201 ccataaatag aagtaaaatt aactccttgt actctagcaa ccaatttcac ccatacatct
     1261 gggtccaata ttctgtataa atttaaactc tcttttgctg aatcattaaa aggtgatgcc
     1321 actctcattt ttcttggagc tactccagct ttatagaata agtcataaat tttattatag
     1381 tcatagtcaa atttatagtg tgctaaccat atatcttcta tttcccaatc atatatagga
     1441 gatgccgtcc agacattttt aaattcctta gttatccagc actttccttt atatccatgc
     1501 tgtttattca caatagaaca ataacgttgt agagattctg ctgctcgtgt tcctaataag
     1561 caaacaactt ttgcaccttc atgtatatct ctaatccatc taccaaattg ttttgccaaa
     1621 ctttcttgtc tcattttata tttataataa gtaaaaggat tatttttttg atttattatg
     1681 tattctttga ctggcatttc ccttacccat aactcttctt tctcatcatc ccatggatac
     1741 caatacattt cataatttcc tacagcagtt cttgtagcca ttggtaaaca cacccaatat
     1801 ggttcaatat cttcaatatt atcttcaaat actttttcaa catattcagt agtcaaacta
     1861 tattgagcct caaaatcttg atgaaatact cctattttgg aacttatatt attctttctc
     1921 ttataatcca aaactaaatt tagcagtaaa ccactatctt ttcctccgct aaaagataca
     1981 tagatataat caaattcatt aaaaagataa gaaagtcttt cttgaaaagc ttcaaataca
     2041 tcggattcta aatatctctt atccatttga attatcccct ttcaattaga agtttataaa
     2101 accttacaat gcaatattat tatgcttttt tagcagtaat cagcataaat tatacattat
     2161 ttaaatacac aaatccaact aactacatat aattataatg taattatact tttgtattat
     2221 aaactatatt ttatcattat tttacatttt tttccaatat atataaatat catatttttc
     2281 tattagctaa aactaattac tttatatttc tacgtattag tataataata cataaaaact
     2341 tgaataatgc ataaaaactt gacatactgt tgtcataatt actttgctat aattaaaagt
     2401 aaaaatggag gtaaattcta atgtatgaac gtatgcttga taaaagcaat aaaccctgtt
     2461 tgtctcaaat aatagaatat ataggtaaag atggatatgc acgattgtgt aagttggaaa
     2521 cttacttgaa aactcaatat aatttatcaa gagaacttcg atttcctttt ggaaacaatt
     2581 atggttgggg ttacaaatat agccataaat cttttcattt attttatgct ttttttgaaa
     2641 aaggtgcatt tactgttctg attcagatag gtgacaagca agttccagca gtagaaaaaa
     2701 cgttggtagc tttgacacca aaagctaaag agctctgggt cactcgttat ccttgtggca
     2761 ataatggagg ctggattaag tatcgtattt tagaagatgc agacttactt gatattattc
     2821 aatatataag agcaaaaaaa tctccaccta aacaaaatta acttttattt tttgtgattt
     2881 aaatatagtg tatataaatt ccattctttc catataaaaa aaataatata aaaaatatct
     2941 attagtataa cttatttagt atgtcttatt ctacttttta cagataatta aaaagtttta
     3001 tcataaataa atcataacta gacatcctag taaatcaaaa ttaatttatt cttttttatt
     3061 agaatttttg ataacagtcc ctatacatat gccaattatc attccaaaaa ttagaccata
     3121 acacattgtg atacctacat ttcctttaga aatacctata gcacaaccta tagccattcc
     3181 aagtatcatg cctataaaca tacatataat aactaaattg ttgtttttat tcatagtttt
     3241 ctccattcaa tatattttaa attgcctagc tcgccttctt agcttaaaac aaagaaatat
     3301 aaattaactt tctttcattc taatatctgc ttgtataaat gtcaaacaac acatttcttg
     3361 tcataacctc ctctttttga agtaagtaca atctcccaga cgcaataata tcaatttcct
     3421 aaaaccattt aagtaacttt tatatttaca taatatttac aatcataaac tatattttta
     3481 ttcatttata catcataaac actccctact aatcattatt atacttaaaa taatgttcat
     3541 ttataataat atatctaaca cactttaatt gaaaaaaata aaactttgcc tttcattccc
     3601 atatataaat aagtatactt tttgatgctc tattacaaat caactaaatt aattgatata
     3661 aattatcatt ttctgttgaa aataccattc gcacatgcta tacttaattg cataaatggt
     3721 aataaatgtc atatgatttt acaaatctat taataacaat tattactttt actgtatttt
     3781 aaattttttg gactaaatat caagaacttt ctaaaataca aagtacaaaa aatttatata
     3841 agttataaat ttatatgaac tagataagct caaacaatat gttatgtata aactgtgtag
     3901 tattcggtta taaatttata taaattatat aagctagata aagtattacc tcaactatat
     3961 aagctagata tataaattgc atatataagc tatataagaa ttatttcaac tatataaact
     4021 acatatgt
//
LOCUS       DBGJFU010000216         3936 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.220, whole genome shotgun sequence.
ACCESSION   DBGJFU010000216 DBGJFU010000000
VERSION     DBGJFU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.220"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            129..1784
                     /gene="ilvD"
                     /locus_tag="ACOIAS_003664"
     CDS             129..1784
                     /gene="ilvD"
                     /locus_tag="ACOIAS_003664"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGZ2703331.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLNFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2128..2760
                     /locus_tag="ACOIAS_003665"
     CDS             2128..2760
                     /locus_tag="ACOIAS_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2703332.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            2783..3595
                     /locus_tag="ACOIAS_003666"
     CDS             2783..3595
                     /locus_tag="ACOIAS_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2703333.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1525 a          446 c          737 g         1228 t
ORIGIN      
        1 ataaaaaatc gtaataaatt ttcattagta tatgctaata tttttttaca attttcgtaa
       61 ttaattaata gaaaaatata ttataataat attatgaaaa tttaaaaaag taagaaagag
      121 gtaatattat gagaagtgat ataaaaaaag gaatagaagg agctccaaag agagcgttaa
      181 tgtatggaat gggtcttact aaagaagaaa ttgaaagacc attaataggt atagtaaatg
      241 cacaaaatga ggtgattcct ggtcatctgc atcttgatga aatagcagaa gctgcaaaaa
      301 atggagtaag aatgagtggt ggtttgccat tagaatttcc tgctataggg gtatgtgatg
      361 gtatagctat ggggcatgtt ggaatgaatt attccctggc ttcaagagaa ttaatagcag
      421 attcaataga agctatggct atggcacatg gttttgatgc tttggtactt atacctaatt
      481 gtgacaagat agtgccaggt atgttaatgg ctgctgcaag acttaatata ccaagtattg
      541 ttgtgagtgg agggcctatg cttccaggta aaaaaaatgg aaaagtatat gattttaact
      601 ctgcaatgga gggtgttgga gcatgtaaag atggaacagt aagtgaagaa gaattagaag
      661 cgttagccat gaactcttgt cctggatgtg gttcatgttc tggtcttttc acagcaaata
      721 gcatgaattg tttaacagaa gctttaggca tgggtatacc atataatggt actgctgcgt
      781 cacattctgg agaaagaaaa aggatagcaa aatatgcagg tatgtatgtt atggagttac
      841 ttaagaacga cataaaacct agagatattt taacaataga tgcttttaaa aatgctatag
      901 ctgtggatat ggcaatggct ggttctacaa atacagtact tcacttacct gcaatagctt
      961 atgaatcagg aatagagctt aacttaaatt tttttgatga aataagtgaa aaaactcctt
     1021 gtttaacaaa attaagtcca agtggaaaac atcatattga agatttacat atggcaggag
     1081 gaataccagc tataatgaac gaactttcaa agataaatgg aataaattta gattgcaaaa
     1141 ccgtaacagg caagactata ggggaaaata taagaaattg tgaaatagaa aatgaagaag
     1201 taatacatac attaaagaat ccatatagta accaaggtgg gcttgcaata ttgaaaggaa
     1261 atcttgctct aaatggagct gttgtaaaaa agtcagcagt tgcagaagaa atgttagttc
     1321 atgaaggacc agcaagagtt tttaattcag aagaagaagc tgtaaatgct atttttggta
     1381 aaaaaataaa caaaggtgat gttatagtta taagatatga aggtccaaag ggtggtccag
     1441 gaatgaaaga aatgctatct cctacatcag cagttgcagg aatgggactt gataagcatg
     1501 tagcacttct tactgatggt cgtttttcag gagcaactag aggagcatct ataggccata
     1561 tttctccaga agctatggaa ggtggtttaa tcgggttagt tgaagaagga gatataattt
     1621 ctataaacat accagacaaa aaattagagt taaaagtaga tgaagttgaa atagaaaata
     1681 gaaaattaaa atttaaacct ttagaaccga aaataaagca tggatactta agtagatatg
     1741 ctaaactggt aacatcagca aatacaggag cagttttaaa atagtagact atatttatgt
     1801 aaattagaaa aagttaagaa aatattaatt ggatatctta ggtgttatag cactaatgag
     1861 gtatcctttt tattttgtat gaaaaacaca gcaaaatctt gattatcttt gaaaacaata
     1921 catctctatt ataaggaata cgagtacagc atagagatgt atcttttaga ccttgttgag
     1981 gtaatacaac tttttaaaag tttgatgtgg aatgcttttc gttccatctg aactatgaag
     2041 aaattgaaag tcagttaatt cttgacataa taaatattaa aatgtataat aaaaaacaaa
     2101 cagacagtct gtttttagga gggaaatatg aatagagaag aaaaaagtaa aaatagtaaa
     2161 gaaaaaatta tccaatcagc attttcacta ttttcgtcta aaggatatga ttcaacatct
     2221 acacaagata ttatcaattt atctggtcta tctagaggtg caatgtatca tcactttaaa
     2281 actaaagaag atatactgag aagtgtcaca aaagaacttt actcacaaat gaataatttt
     2341 ttagagcatc ttgttgctga tgacaccctt acagcaaatg aaaaaataat agaattggtt
     2401 gttcatagtg cgaatgatta cacacgtaga aaaatggtac attgtagctg gttagaaaaa
     2461 atcccattcg ctttaataga ggaagttcgt aatctcaaca atgtagttgc acccaatatt
     2521 gctaagataa ttaaacaagg tgttgaaaat aaagaatttt cttgtgaata tccagaggaa
     2581 ttagctgaaa tgctcgtttt tagtattgat attttacttg accctgtatt atttaaacgt
     2641 gaatacagtg aagtatgtaa taggttggat tttttattat tcatgttaaa aaaaatggat
     2701 attcctctaa ttgatgagta tggtattcaa aaatttaaag atttatttag acaattataa
     2761 taggaaaagc gaggaataga ctatgtttta taatgcaaaa agttgtcaaa tgatgatagg
     2821 aagtaataca gtgaattata ttgaatttgg tagtggtaag aagacactaa ttattttacc
     2881 aggattagga gatggtttat ttcctttaca tggaaaaata caggcgattg cttttgcttt
     2941 tagatataag caatttgcaa aagattataa ggtttatgta tttagtagaa aaaatcaaat
     3001 tacagaaaaa tattccacaa gggatatggc taaagaccaa gcagatataa tgaaaaaact
     3061 tggaattatg aaagcagaag taatgggtgt ttctcaaggt ggaatgatag ctcaatatct
     3121 ggcaattgat tatcctgagt tagttgagaa acttgtgttg gcagttactt cttcaaagca
     3181 aaatgatact atccaaaatg ttatttgtag ttggattgat atggcgaaga agcagaatta
     3241 caatgattta atgattgaca cagcaaagaa atcctattct gaaagatatt taaaaaaata
     3301 ccaattattt attccttttt taggaaaagt aggtaaacca aaggatttta aacgatttat
     3361 tattcaagcg acctcttgca tagaacataa tgccttttcc gaattaaata aaataacatg
     3421 tccaacctta attataggag gtgctaatga taaaattgtt ggaaataatg cttcttttca
     3481 tttagcagaa aagattaaaa aaagcgaaat atttatctat gagggattgg gacacgctac
     3541 atatgaagaa gcacaggatt ttaatgaaag agttttagag tttttaaata agtaaacacc
     3601 tttgttaaaa atatacatat gaagattgga aatttaatgt taaaaataga aacatgaaaa
     3661 tatgcttaac tggtattttt actattcata accaattttt gatacattat ctactataaa
     3721 tacaaatata gcttcaatgt gattatatat tagtgtattg gtaaagcact tatacaaaca
     3781 gaggaatttt gtaaattcat attataccca catttgttaa cttatgaaaa tataatcaaa
     3841 atttttatga gatttttata taaaaaatat aatgtttgta tacaaaaata ttgaagatat
     3901 tttagtaagt tttgtatata agcaaacatg tatttt
//
LOCUS       DBGJFU010000217         3888 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.221, whole genome shotgun sequence.
ACCESSION   DBGJFU010000217 DBGJFU010000000
VERSION     DBGJFU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.221"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..940
                     /locus_tag="ACOIAS_003667"
     CDS             <1..940
                     /locus_tag="ACOIAS_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703334.1"
                     /translation="LYKYKKDNKIIFDKIIELIDKDINIEMYYKNRNSYIEDRTSVDI
                     IRESDFKKLRQYQNNNYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDI
                     NLFKVQKSILEPEINSQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTL
                     KLKNIVSENMKFTYPFNFTSSIESYDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQ
                     LSNIYDDKERYIMLIKLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFI
                     PRSLLVPIIITLVIYFIGYFIYYFLTLKYALNMIKE"
     gene            1165..2778
                     /gene="ggt"
                     /locus_tag="ACOIAS_003668"
     CDS             1165..2778
                     /gene="ggt"
                     /locus_tag="ACOIAS_003668"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGZ2703335.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2911..3762)
                     /locus_tag="ACOIAS_003669"
     CDS             complement(2911..3762)
                     /locus_tag="ACOIAS_003669"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HGZ2703336.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
BASE COUNT         1515 a          475 c          609 g         1289 t
ORIGIN      
        1 attatataaa tataaaaaag ataataagat aatctttgat aaaataatag aacttataga
       61 caaagatata aatattgaaa tgtattataa aaatcgaaat agttatatag aagatagaac
      121 gtctgtagat attattagag aaagtgattt taaaaaatta agacaatatc agaataacaa
      181 ttatgaagaa ttaacttcaa aggaatctgt gtactatgtt tctgattcat acaaaaaaat
      241 attttttaaa gactttgaga ttgaaaaaat aaacttgcat attaaagatg caaatgagta
      301 tgatataaat ctgtttaaag tacaaaaaag catcttggag ccagaaataa attcacaagc
      361 tacgtttgat ttaattgtag taaaagatga cgtatttaat cagttaaaat tacatggaaa
      421 ttctagatta ataagattaa ttgatataaa aaatgaaaaa aaagaactgg atttgacttt
      481 aaaattaaaa aatatagtaa gtgaaaatat gaaatttact tatcctttta attttacatc
      541 aagtattgaa agttatgata acttgataaa attaagtggt ctaatgctgt ttataggcat
      601 atttttgtct gtagtatttc ttttatgtac aggtagtata atcttattta aacaattatc
      661 aaatatatat gatgataagg aaagatatat tatgttaata aaacttggag caaataataa
      721 agatattgag aaaataatat caaaacagtt aaaagttata tttttaatgc cattagttgt
      781 aggtacagtt cataatttat ttgctatgtc tatagcacaa aagtttatac caaggtcatt
      841 acttgtacca attatcataa ctctagtcat ttattttata ggatatttta tatattattt
      901 tttaacttta aaatatgctc taaacatgat aaaagaataa attattttta agttaaaaga
      961 attagatata tgttttgaat aaaataactt atttaagtgc agatagaaaa tactagaatt
     1021 gagtttaaga tatttattta aagataaaat taaagttagg aaaaagtcta tattttatat
     1081 cattttgtat cagttacatt aattgatgtt atataataaa tctaagcaga atatcaaata
     1141 aaaaatgacg aggagctgaa atttatgaag tttaatgcct accaacataa atactcatca
     1201 ataagaaatg tcgtgtatgc taaaaatgga gcagttgcaa catcaacacc attagcatct
     1261 caagctggac ttgaaatact aaaaaagggt ggaaatgctg ttgatgcagc agttgcaaca
     1321 gccgccacat tagcagtagt agaacctaca agcaatggga ttggaggaga tgcatatgcc
     1381 cttgtttgga tagaggaaga aaaacgattg tatggtttaa attctagtgg ctttgctcca
     1441 gaaaatatgg aattaaaaaa ttataataat atgaaagaaa tgccaaaata tgggtttgga
     1501 gcagtaactg tacctggaat accagcagct tggtcagaat taaataaaaa atatggtaaa
     1561 cttagcttaa tggaatgctt atctccagcg ataaactatg caagagaggg atacgtagta
     1621 tctcctaatg tagctaaggt atggaaaaaa tcatatgaac tctatgaaaa agaacttatt
     1681 ggagaagagt tcaaaccatg gtttgataca ttttctaaag atggtaaagc tccagaggct
     1741 ggagatatat ttatatgtga agaacaggca agtacattag aagagatagc aaatactcag
     1801 gctgaaagct tttatagagg aagacttgct gataaaatag atgaatattc taaaaaattt
     1861 aatggggcta taagaaagag tgacttagag tgtttttatc caacatgggt agaacctata
     1921 agtacagaat ataagggata taaaatattt gaaatcccac caaatggtca tggaataaca
     1981 gttttaatgg cacttaacat attaaaagaa ttggaattag aaggaaatat agaaaatgta
     2041 gaagacatac ataaaattat agagagttta aaattagctt ttgcagattc taaaacatat
     2101 gtaactgata tagagcatat gaaggtaaag attcaggaat tactaagcca agagtatgct
     2161 aaaaaaagaa gtttattgat tgacaataaa gaggctttgt atccaactgc aggagagcca
     2221 tattgtggtg gtacagtata cctttgtact gcagataagg atggaaatat ggtttcatat
     2281 atacagagca attacataaa ttttggttca ggaattgtga taccaagaac tgggattgct
     2341 ttacatagta gaggaaataa ttttaattta gaccctaaac accacaatgt agtaaaacca
     2401 tttaaaaaac cttatcatac tataatacct ggatttttag gtaaagaaga taaagcaata
     2461 ggtccatttg gtgtaatggg agcttttatg caaccacaag gacatattca agtgttaaca
     2521 aatatgattg attttggatt aaatccacaa gaggcgctag atgctccaag atggcagtgg
     2581 ataaaaggaa aggaaataga agttgaacca gagatgccaa agcacataat agattcatta
     2641 attgaaaagg gtcatgaaat aaaagtaatt catgatactg tcgacatggg acgaggtcaa
     2701 ataatattta aaacagaaca ggaaagttat atttgtggga cggaatcaag atgtgatggt
     2761 catattgcag tttattaata ttatcaacta aaatatatat aataaattta aagagtatat
     2821 gaaataatta gtaaaggcga aagtctacaa tgactgacgc ctttacaaaa tttaagctat
     2881 catatgctga ttttatatag attatgagcc ttattcttga ccagttttga tttcattgtc
     2941 atcataacta atccagtcac taaagctacc aatataaatc ttgtgaggta tatccagttc
     3001 tctaagagca agactgttaa cacaagcaga tatccctgaa ccacaagata aaattacttc
     3061 atcataatta tttaaatctt taaagaattt ttttaaaaat tctatatctt taagtgagcc
     3121 attttcaaag tcacttttta ttaaatcctt acaaaaataa ttttttgcac ttggtatatg
     3181 accagctttt gagtaagcag gttcaactaa accctggtat ctctcattag cacggcaatc
     3241 tattattaca gtatcttttt tgtataattt agacttaaca tattccattg gaacaactaa
     3301 accattgttt atatttggtc taatttcctt acctgtacaa cttggtatat tcactttctc
     3361 ttccaattct ccaccttcct ttacgaaaga tgttattccc ccatccagta catagacatt
     3421 tttaagcccc aaatgtttta attggaaaaa taatctgcaa gctccattta aatctccatc
     3481 atcataagtt acaatcgtag tgtcgttatc aacacccatt ttttctaatt tttcttttag
     3541 tattaaaggg tcttgtaaag gatttttacc accatgttct tttgtaggag atgataaatc
     3601 cttgtctata tctaagataa agctgccttt gatgtgtcct tttttataag aatcaatacc
     3661 ataagttcta ttgataagat caaatctgca atctattaca actagattat catttttttc
     3721 tagttttgat ataagttctt ttgctgaaat aatgtttttc aaagaatgta cctcctgtgt
     3781 ttatattatc tttattttaa tttatattaa ttattaactt tataaactaa tattattata
     3841 atttattaca taagtaaata aatctttaga aattgtaaaa attttata
//
LOCUS       DBGJFU010000218         3836 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.222, whole genome shotgun sequence.
ACCESSION   DBGJFU010000218 DBGJFU010000000
VERSION     DBGJFU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.222"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            380..1756
                     /locus_tag="ACOIAS_003670"
     CDS             380..1756
                     /locus_tag="ACOIAS_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HGZ2703337.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            2043..2714
                     /locus_tag="ACOIAS_003671"
     CDS             2043..2714
                     /locus_tag="ACOIAS_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HGZ2703338.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2903..3700
                     /locus_tag="ACOIAS_003672"
     CDS             2903..3700
                     /locus_tag="ACOIAS_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HGZ2703339.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDVNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1485 a          415 c          692 g         1244 t
ORIGIN      
        1 atataaaaaa tgcatgttaa gaaaatttaa cataaagata tagataagat tataaataat
       61 ataaaaaatt taacttcaaa aaagtatatg aaatttatac tggtaaaatg ataaataaat
      121 tttaagtaca aaatagtaaa agctatgata gttttagacc tattatatgt aaaaaatata
      181 gatgtttttg gtgcaattta aattaaaaga tatatataaa aatttaaaat attccaaaca
      241 gaaattataa aaattactat cattgtcttt atacaatatt tatactataa tagaataata
      301 atttagagga aagctctaaa aataaacaaa ttaagggtct taacagtagt gattttatgt
      361 taaggaggta taaagaaagt tgaaaacaat agttagaaga cttattctct acgcagatgg
      421 aatgcgtcct actcaaataa tggtatctgg gtttgctgcg attattgtaa tcggagcatt
      481 gttgttaaca ctcccgatag cgtcgcagag tggagagagt ataggcttat taaatgctct
      541 atttacggct acttcagcgg tttgtgttac tggtctagtt atggtagata cagcaactta
      601 ttggagttta tttggacaga tagttataat aacattgata caaattggtg gtcttggatt
      661 tatgaccgta gccacaatgt tttcacttat ggcacgaaaa aaaatacaac taagagaaag
      721 attattaata caagagtcat taaatcaagc tgatttatcg ggacttgtaa gacttacaag
      781 atttgtatta ataataacca taacaataga gggaataggt gcactggttt tatcaactgt
      841 ctttattcca caatttggtt tgtctaaagg gatatggtat agtgtatttc acgcaatatc
      901 agcgttttgt aatgccggat ttgacttgat gggcagtgta agtggcccat ttacatcgtt
      961 aaattcttat gtaaacaact ttacagtatc aatgactgtt tgcgcactta tagtgcttgg
     1021 agggctagga ttccctgttg tacttgatat tgtaagaaaa agaagatttt caaaattaaa
     1081 tgtgcattct aaggttgtat tgttttcaac tgctacactt atttttgtgg gtgcattatt
     1141 tatttttctt atagaattta atcaaaaagc tactatggcg gacttgcctt taaagggaaa
     1201 agtactatca gcaatatttc aatcagtaac agcaagaact gctggtttta atacgcttga
     1261 tttagctact ttacgagaaa gtagtgtatt tgtaatgata attttgatgt ttattggagc
     1321 atcacctgca tcaactggtg ggggtataaa aacaacaaca ctagctgttt taatcattac
     1381 agttagaagt tttctatcag gaaaatcaga tatagaagct tttgaaagaa gattagctcc
     1441 atcaactata aaaaaatctc ttggtatatt tgtaatcagt atatcagcag ttatttttgg
     1501 aacattgatt atttcaataa ctcagcctaa ttttacatta gttcaatcag cttttgaggt
     1561 tacatcagca cttgctacag ttggttcaag tttagctgga actccaaact taaatgcttt
     1621 aggtaagatt ataataataa tctttatgtt tatgggtaga gttggttctc ttactttatt
     1681 tatggctata ctttctggtg gtagaagaaa gagtcaacca atcagatatg cagaaggtaa
     1741 aattatggtt ggttaagtta ccaaggagta atataaattt ataatggatt tagagtagca
     1801 ctgtaaatct gttgataaaa taaatgagcc tagatgtaat tatatgaaga aataaaatcg
     1861 actaacgaac aaaattaatt aatgaaataa aaaaacaggg aaataaaata caaataaaaa
     1921 ggtaaatcaa aattaaatta atctgtgtat ataaaattat atattgtgta taagttttgt
     1981 atgtgtaaac aattaattta agagatatta tataaattgt gtattaggga ggaagtttcg
     2041 agatgaagca atatatagtc attgggtgtg ggagatttgg aagttcagtt gcgtctacta
     2101 tgcatctttt aggacatcaa gtaatggcaa tagacaaaaa tgaagattca gttcaaagta
     2161 tatctgacaa ggtaacccat tcacttatag tggatgttac tgatgagcaa gcgttaaggt
     2221 cattaggttt aggtaacttt gatgtagcag tagttgcaat aggttctgat ataagggcat
     2281 ctataatggc gactcttata gccaaagaaa tgggtgtaga gttgataata tgtaaggcaa
     2341 aggatgaatt acaagctaaa gtgctttata aaattggcgc agatagagtt gtatttccag
     2401 aaagagatat gggagtaaga gttgcacaca atttagtttc agataatata ttagaccata
     2461 ttgaacttga cccagagtat tcaattgttg aaatcgtaac tccaaatagt tgggttggca
     2521 agacacttat agagcttgaa ttaagagcta gatatgagat aactgtactt gctataaaaa
     2581 caggtaaaaa tataaatgtt acaccttctc cagatgagga acttacagcc ggaagtatcc
     2641 tagttataat cggtcaaaat actagtataa cagcgataac atctggaaat aaggggataa
     2701 ttagaagaag ataatttact atttaatata tatttaattg caatgaaagt agatagtatc
     2761 atataattat gaatagttat atgatactat ttttttaatc gaaggtagta tttatttgta
     2821 agattagata aagagaaatt aaattaaaag taaggaggct gtgctaatat aaaaatttat
     2881 aagttattag catttagata aaatgattac aaatataaac agtaaggata atgaaaagtt
     2941 aaagtataca agagcactat taaaatcaaa gaataggaat aaagagtcaa agttcataat
     3001 agagggatac agaatagtaa tgcttgcact tgaatgtatg gcaaaccttg attatgtatt
     3061 tatcaatgaa gaatttgaaa ataagaaaga acatgtaaaa ctattagaag atttggataa
     3121 aaaaaacaca aagatataca agactactaa taaaaacttt aaagaattag tggatacaga
     3181 aaatactcaa ggaataatag gtgtagtttc atttaagaaa aaaaaattaa gtgaaagtat
     3241 aaataaaaaa gataaatttg tattgatttt agacagaata caagacccag gaaatatggg
     3301 gactataata aggactgctg attctgctgg agtagatgcc ataatagcac taaagggatg
     3361 tgtcgatata tacaacccaa aagtaattag gtctactatg ggttctattt ttgatgtgaa
     3421 tataattgat gcttcacaag atgaaactgt ggacatgctt aaatcattgg attttaatat
     3481 agtttcaagt tacttaaata cagaaaattt ttatgacaaa atagattatg gctcaaaagt
     3541 agcattggtg ataggaaacg aagcaaatgg aataaatgaa gaacttgtat caaagtctga
     3601 tattttggtt aagataccta tatatggtaa agccgagtcg ttaaatgctg cgataagctc
     3661 tgctatactg atgtatgaaa taaaaaaata cttaatttaa tgtattgtaa taaatataat
     3721 gtcatgttat aatcttgaat taaatagtag agtatcaaaa atagtcaata tatattaaaa
     3781 aaataattaa attaattata tattagatgt atattatata aaaaataatt gagtta
//
LOCUS       DBGJFU010000219         3693 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.223, whole genome shotgun sequence.
ACCESSION   DBGJFU010000219 DBGJFU010000000
VERSION     DBGJFU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.223"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..1042
                     /gene="ytvI"
                     /locus_tag="ACOIAS_003673"
     CDS             <1..1042
                     /gene="ytvI"
                     /locus_tag="ACOIAS_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HGZ2703340.1"
                     /translation="ENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAIIALM
                     INPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNISANPE
                     DISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLATSIP
                     YIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYIRAYT
                     ILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLIKNYF
                     IAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFCLGLV
                     CMHDILKKVDIF"
     gene            1132..2358
                     /gene="pepT"
                     /locus_tag="ACOIAS_003674"
     CDS             1132..2358
                     /gene="pepT"
                     /locus_tag="ACOIAS_003674"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGZ2703341.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPKIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2565..3617
                     /locus_tag="ACOIAS_003675"
     CDS             2565..3617
                     /locus_tag="ACOIAS_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HGZ2703342.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADNISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1427 a          386 c          685 g         1195 t
ORIGIN      
        1 agaaaataat tttttataca aattaaaaaa caacactatc tttttttgta tatatacatt
       61 gttatttatt tttatttaca aagcgtttcc atacatagct ccattttttc taggtgctat
      121 aatagcactt atgattaatc ctatatcaca aaagttagaa aataagtttc atataaataa
      181 aggtatctct actcttgttc taagttttct tgcagtggct atagtcagta cagttactac
      241 aataatagta ataaattcaa tgaaagaatt aatgggattt ttaaataata tatctgctaa
      301 tcctgaggat ataagtaata ctattatgta ttttttaaat aaaataaatg actttatgaa
      361 atcatttcaa gaaatagcca attttgatgt agaacaatta gtaaacaaat tcagtggtga
      421 agtaatgcaa attacaaaaa atcttcttac tagtatactt ggattggcta cttctattcc
      481 atatattata atatttataa ttaccttgtt tattgctacg tattttatag caaaagacct
      541 tgataagatt gaaaatagtt tttataatat gtttactgtt gatgttagaa agaaggtaaa
      601 aaacgtaaag aaagaggcag ggctttcact tgttggatat ataagggcct atacaatact
      661 tatggctata acattttttg caatctgggg aagttttgct ttatttggat tgaaatacgg
      721 tctaatagta gggtttgtag gagctttaat ggatttaatt ccattcctag gtataataac
      781 aatatatctt ccagtaattg tttattattt tcttataaaa aattatttta tagctataag
      841 tatgactgtg atattttttg ttttgtcttt gattagagaa atacttgaac ctaaactagt
      901 atcagtaaat gttggactaa atccattagc taccctagca gcgattttta ttggaatcca
      961 agtaaaagga ataattggag taattttttg tttgggtttg gtatgtatgc atgatatact
     1021 taagaaggtg gatatttttt aattataata attgatagat tttttaaaaa taaaatttta
     1081 ttcacaggta aataaaaaaa tattaatctt attaagaaag agggtatata cgtgaaacaa
     1141 aaagtagtgg aaagattttt aaaatatgta tcatttgata caacatctaa ttctcagtgt
     1201 gagaactgtc catcttcaga aggtcaaaga gttttagcta agtatatagt tgaagagcta
     1261 aaaacaatgg gtgttgatga tgtgagttta gatgaaaata gttatataat ggctacttta
     1321 aaaggaaata ctgatggtgt ggatactata ggattcatat cacatttaga tacaattgaa
     1381 gatgttagtg gaaaagatat taaaccaaaa ataatagaaa attatgacgg aaaagacata
     1441 gttttaaatg aagcgttaaa tgttattacg tatgtaaagg attctccaga gttggaagaa
     1501 tttaaaggtg acgatttaat agtcactgat ggaacaactc tacttgggtc tgatgataaa
     1561 gctggtatag ctgagatagt gactgcaata gagtatctaa taaatcatcc agaaattaaa
     1621 catggagata taaaaatagg atttacacca gatgaagaaa ttggtagagg tgctgattta
     1681 tttgatgttg aaaaatttgg agcaaaatat gcatatactt tagatggagg aatagttgga
     1741 gaacttgaat gtgaaaattt taatgctgca aatgcaacta ttactataca tggtagaaat
     1801 gttcatccag gttcagctaa aaataaaatg gtaaatgcta tacatatagc tgctgaaata
     1861 tcagaaatgt tcccagcaga tgaaagacca gaaacaacag aaggatatga aggattttgg
     1921 catttaaatt ctataggtgg aaatgtcgag aatgtaagta tggcttatat tattagagac
     1981 cattgtaaag aaaaatttga aaatagaaaa tctattatga tagagaatat agaaaaaata
     2041 aataagaaat acgataatag agtggaattg gatttaaaag actcttatta caatatgaaa
     2101 gaaaagattg agccagttat gtttatagtt gatatagcta aagaagctat ggaggaatta
     2161 ggtataaaac cacgtttagt tcctgtaaga ggtggtactg atggagcaag attatctttt
     2221 aatggattac cttgtccaaa tatatttact ggtggattaa acttccatgg gaaaaatgag
     2281 tgtataccag ttagttctat ggagaaagct actaaactaa ttgtaagaat agctgaaaaa
     2341 tatgctgaaa gagtttagtt aaaaagtatt taaaaattaa gcatttaaag ttataatagc
     2401 tatatcattc tttataagtc aagctgatta taagcttgtg ttgtacttaa aatggtatag
     2461 ctatttttgt aattattttt ataaaagata attattttta gtttagggta tcaataaaaa
     2521 gtatgaatat caataggtat taacatttaa aagggggaat tgtattgaaa attaaatcta
     2581 aaaaaataat agcatttgca ctttcacttg ggattatgtt gccaagttgt gtatatgcag
     2641 acaatattag taaaaatcaa aatgcacagg tagatatgaa caattatctt caaggtagaa
     2701 aatctccaaa ggaggaaaaa cttattggtg aagatagatt tgatacagcg ataaaaatat
     2761 ctcaaagtgg ttggaataat ggttcagaaa gggtgtttct agttaactca aattcattgc
     2821 cagatgcatt ggcttcaact cctttggcaa gtaaattgga tgcaccaata ctgcttacaa
     2881 ataaaaatag tataccatat aatgtaacag aggaaataaa aaggcttaat ccagcagaag
     2941 ttattttaat tggaagtgaa ggagctattt catcaagtgt aaaagaatct cttgaagata
     3001 tggaagtatt agtcagtaga atcggtggag cagatagaga agaaacatct ttacttctta
     3061 ctagacagct agatgatact ggtgatttag gtgtctcaaa agtggcagtg gttaatgggt
     3121 ataatggact ggcagatgca acttcaatat caagcccagc tgcaagtgat aatacagcta
     3181 taatatatac aggtaaggat agcataagaa gtgaggcaaa gaattttatt acacaaaact
     3241 caactaagac atacatagta ggtggggagt acagtataag taaaaaactt gaaggacagt
     3301 tagtaaactc agaaagacta gcagggacag atagaaaaga tactaatgcg aaagttttag
     3361 aaaagtttta tggaaaatca agtaaggtta ataatatgta ttttgcaaaa gatggaagtg
     3421 gtagagaagc tgatttagtt gatgggcttg ctgctggagt attagcatca aaaacaaagt
     3481 cacctgtagt acttgcaagt ggaagtttaa gtagtgcaca gaaaagtttt ataaaaaaag
     3541 taaaggctga taaatttgta caagttggtg gaggtaaaaa tagtaaacca tatgcagaag
     3601 cattagcctt acagtaatat tgtgtaaatc aaaattaata tataataata gcaagtaaac
     3661 caagttgcac tttaatatat ataataaaat aag
//
LOCUS       DBGJFU010000220         3686 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.224, whole genome shotgun sequence.
ACCESSION   DBGJFU010000220 DBGJFU010000000
VERSION     DBGJFU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.224"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..123)
                     /locus_tag="ACOIAS_003676"
     CDS             complement(<1..123)
                     /locus_tag="ACOIAS_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGZ2703343.1"
                     /translation="MRKTKETHTFDFRPLGLAIREAREKAGLSRNDLGDKVFYGE"
     gene            261..620
                     /locus_tag="ACOIAS_003677"
     CDS             261..620
                     /locus_tag="ACOIAS_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2703344.1"
                     /translation="MKLRESYTCPLELVHDIIKGKWKTIIIFQLRNGKCSFSELHHSI
                     AGITQKMLLEQLRELKHFGLIDKKTYDGYPLKVEYFLTGQGEKMLSAIKIMQEIGIDY
                     MVEHGMTEILDEKGICY"
     gene            710..1261
                     /locus_tag="ACOIAS_003678"
     CDS             710..1261
                     /locus_tag="ACOIAS_003678"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGZ2703345.1"
                     /translation="MKKALLIIDVQNDYFPNGKCQLYKPEIALNTIKDLLKIFRKQNL
                     PIIYIRHESIHGTFFNPNTNGVQIHNDIKPLDTETVIVKHYPNSFYETNLQNKLVEIE
                     VTKLVVCGMMTHMCIDTTIRAAKDYGYEITLISNGCATKELKWNGVNIPADTVQSIFM
                     ASLNQKFANVITSTEFLSNEITR"
     gene            1245..1610
                     /locus_tag="ACOIAS_003679"
     CDS             1245..1610
                     /locus_tag="ACOIAS_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGZ2703346.1"
                     /translation="MKLRDEYTCPLELIHDMIKGKWKPIILWRLRLGATSLSKLENDI
                     DGITQKMLLEHLKELIDYGFVDKKTFSGYPLHVEYFLTEDMGNQILEALRIMQHIGIE
                     YLRKNGMENELTEKHVIPD"
     gene            1698..2198
                     /locus_tag="ACOIAS_003680"
     CDS             1698..2198
                     /locus_tag="ACOIAS_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HGZ2703347.1"
                     /translation="MYISSIGISNGIIEKKYGSHGVQFNENDVPTYSIPFKIENAPDK
                     TVSFAFILEDKDAYPVTGFIWVHWLGANLTRSELKENESQTAKDFIQGANSWTSILGN
                     QQTREQTSCYGGMAPPDKAHIYELHVFALDKLLDLKNGFYLNELYQEMEGHILEEAVL
                     KGLYEK"
     gene            2188..2703
                     /locus_tag="ACOIAS_003681"
     CDS             2188..2703
                     /locus_tag="ACOIAS_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGZ2703348.1"
                     /translation="MKSKPVKVGLRTIKTVLSAFLCFLIDSLRASSVPFYAVIASILC
                     IQPDMENSLSVAKNRELSTIIGGILGMLFLLMERIFLPIQPEILRELTLSILLIPIIN
                     LSVLLKQKKGTFLTCVVFLCVTVTHEKDISPIWFAWNRIVDTSLGIGISLAVNSFPFH
                     KFKVGGTMGKY"
     gene            2748..2978
                     /locus_tag="ACOIAS_003682"
     CDS             2748..2978
                     /locus_tag="ACOIAS_003682"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HGZ2703349.1"
                     /translation="MEWLSSKLAYTPASFNQQKKGFDIDPLTRTFTNEEGTSIYLTAK
                     EFDLLYFLSSHKGQVFTKEQIYEKVWGYDNFY"
     gene            3132..3322
                     /locus_tag="ACOIAS_003683"
                     /pseudo
     CDS             3132..3322
                     /locus_tag="ACOIAS_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            3346..3570
                     /locus_tag="ACOIAS_003684"
     CDS             3346..3570
                     /locus_tag="ACOIAS_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703350.1"
                     /translation="MIGIKKRDIKKALKAGAKAGGSSYADVLADIEATIDEVMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTRV"
     gene            3592..>3686
                     /locus_tag="ACOIAS_003685"
     CDS             3592..>3686
                     /locus_tag="ACOIAS_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HGZ2703351.1"
                     /translation="MKNEMWVLCPICNNKTRTKVREDTELINFPL"
BASE COUNT         1329 a          501 c          721 g         1135 t
ORIGIN      
        1 ttccccatag aataccttat cgcctaaatc attacgagaa agcccagctt tttcacgggc
       61 ttctctgatt gccagcccta gcggtctgaa atcaaaagta tgtgtttctt ttgtttttct
      121 cattttaaat caccatacat gagtttatac ttcatggttc gtatttagaa tgtgttaaaa
      181 gtgcatatat tagggtaata aaatatgttt tgaaatattg agcttaaaca tgtaagatat
      241 ttcacatagg agatgataaa atgaaactta gagaaagcta tacatgtcca ttagaacttg
      301 ttcatgatat tataaaggga aaatggaaaa cgataattat ttttcaactt agaaatggaa
      361 aatgctcttt ttccgaactc catcacagta tagcaggaat tactcaaaaa atgctattgg
      421 aacaactgcg tgaattaaaa cactttggct taattgataa aaaaacatat gatggatatc
      481 ctctaaaagt agaatacttt ttaactggac agggagaaaa gatgctatca gcaattaaga
      541 taatgcaaga aattggtata gattacatgg tagagcatgg tatgacagaa atccttgatg
      601 aaaaaggaat ttgttattaa actctatcat atacacgaaa aagtaagtaa tttacttaga
      661 aactattatc ttatattatt taattagaat taagagagga tatgagagta tgaaaaaagc
      721 attacttatt attgatgtgc agaatgatta ttttcctaat ggaaagtgcc agttgtataa
      781 accagagatt gcgttgaata caataaaaga tttattgaaa atttttagaa agcaaaactt
      841 gccaattatt tacattcgcc atgagtctat ccatggaaca ttttttaatc caaatacaaa
      901 tggtgtacaa atccataatg atattaaacc attagataca gaaacagtta ttgttaaaca
      961 ttatccaaat agtttttatg aaacgaattt gcaaaacaaa ctggtagaga ttgaggtaac
     1021 gaaattggtt gtttgtggaa tgatgacaca tatgtgtatt gacacaacta taagagctgc
     1081 caaagattat ggctatgaga ttacactaat atcaaatggt tgtgcgacca aggaattaaa
     1141 atggaatgga gtcaatatcc cagcagatac agttcaaagc atatttatgg cttctttaaa
     1201 tcagaaattt gctaatgtta ttacaagcac ggagtttttg tcaaatgaaa ttacgagatg
     1261 aatatacatg tcctttggaa ttaattcatg atatgataaa gggaaaatgg aagccaatta
     1321 ttttatggcg cttacgatta ggtgctactt cattatctaa attagagaat gatattgatg
     1381 gaattacaca aaaaatgctt ttggaacatt taaaggaact tatagattat ggatttgttg
     1441 ataaaaaaac attttctggt tatccacttc atgtcgagta ttttttgacg gaagatatgg
     1501 gaaatcaaat actagaagca ttacgaatta tgcaacatat agggattgaa tatttaagga
     1561 aaaatggaat ggaaaatgaa ttaactgaga aacacgtgat tccagactaa tacctacaaa
     1621 aaagtgggta atttacgttt tgcttcctct ttgttaatat atatctgtaa ttaaacgata
     1681 tacaaggagg aaaatttatg tatatttcaa gtataggaat ttctaatgga attattgaaa
     1741 aaaaatatgg tagtcatgga gtgcaattca atgaaaatga tgtgccaacg tattcaattc
     1801 cgttcaaaat tgaaaatgca ccagataaaa cggtttcctt tgcctttata ctggaagata
     1861 aagacgcata tccagtgacg ggttttatat gggtgcattg gttaggagct aatctaacaa
     1921 gaagtgaatt aaaggaaaac gaaagccaga cagcaaagga ctttatacaa ggtgcgaata
     1981 gctggacaag catattgggg aatcagcaaa ccagagaaca gacaagctgt tatggtggaa
     2041 tggcaccacc agataaagca catatttatg agcttcatgt atttgccctt gataaactgc
     2101 ttgatttaaa aaacggattt tatcttaatg aactctatca agaaatggag gggcacatcc
     2161 ttgaagaagc tgtactaaaa gggttgtatg aaaagtaagc ctgttaaagt tggattgcgg
     2221 acaattaaga cggtactttc tgcgttcctt tgttttttga tagatagctt aagagcttca
     2281 agtgttcctt tttatgcagt gattgcgtct atactgtgca tacagccaga tatggaaaac
     2341 agtttgagcg ttgctaaaaa cagggaactt tcaacaatta tcggcggtat actgggaatg
     2401 ctctttttgc ttatggaaag aatttttttg cccattcagc cagaaatact tcgggaattg
     2461 actttatcta ttttattgat tccaatcatc aatctgtctg tactgttgaa acagaaaaaa
     2521 ggaacatttt taacgtgcgt tgtattctta tgcgtaacag taacacatga gaaagatata
     2581 agtcctattt ggttcgcatg gaataggatt gttgacactt cattagggat tggtatttca
     2641 ctggcagtaa actctttccc ctttcataaa tttaaggtag gtggtactat gggaaaatac
     2701 tgattgttga atttactgaa aaagaggacg ctgttttcca acaagcggtg gaatggctgt
     2761 cctctaaact tgcttacacc cctgcttctt ttaatcagca gaaaaaagga tttgatatag
     2821 acccattaac ccgtaccttt accaacgaag agggaacgtc tatatatctg actgctaaag
     2881 aatttgacct actatatttc ctttccagcc acaaaggaca ggtgttcaca aaagaacaga
     2941 tttatgaaaa ggtatgggga tatgacaatt tttattagtc tggcaataat gaagaaaaaa
     3001 gaattttaga aaagtgaggt gtgcttatgg atttatctat tttggtagta gtattgatat
     3061 tgggtgtggg tattccttta agaaataagg caatcaaaaa atacagataa aatatgaaaa
     3121 agaaaggaaa aatgaaaaaa tatgaatatt tagaaattga ttatactgcg aaaggtgttg
     3181 tattcctttg caccgataaa cataaggaag ttataaatag ctatgcagaa aatgggtatc
     3241 gctatgcggg ttcgtgccta cagaaattga cgcaaaaggc tgtatgcgaa agatagattt
     3301 aatttttgaa aaagaagatt aacgtatttt agggagttgg ttcttgtgat aggaattaaa
     3361 aaacgagata ttaaaaaagc attgaaagct ggtgcaaagg ctggtggttc ttcgtatgct
     3421 gatgttttgg ctgatattga agcaacgatt gatgaagtta tgaacagcac cgacccagaa
     3481 gtgcaggcaa atttcaaaaa gtattttgga aataaacgcc ctacaccaga ggaatatatc
     3541 tataaaatca cgaagaaaac acgagtttaa agaattagta agggtggttc tgtgaaaaat
     3601 gaaatgtggg tattatgtcc tatttgcaat aataagacac gaactaaagt gcgagaagat
     3661 acggaattga taaattttcc tttgtt
//
LOCUS       DBGJFU010000221         3661 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.225, whole genome shotgun sequence.
ACCESSION   DBGJFU010000221 DBGJFU010000000
VERSION     DBGJFU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.225"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..326
                     /locus_tag="ACOIAS_003686"
     CDS             <1..326
                     /locus_tag="ACOIAS_003686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703352.1"
                     /translation="VLLVSINISIVNISVLLVSISISIVNISVLLVSISISIVNISVL
                     LVSISISIVNINVLLVSISISIVNISVLLVSINISIVNISVLLVSISVSIVNISILIV
                     DSSYC"
     gene            complement(575..796)
                     /locus_tag="ACOIAS_003687"
     CDS             complement(575..796)
                     /locus_tag="ACOIAS_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HGZ2703353.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1376..1633
                     /locus_tag="ACOIAS_003688"
     CDS             1376..1633
                     /locus_tag="ACOIAS_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGZ2703354.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            2102..3340
                     /locus_tag="ACOIAS_003689"
     CDS             2102..3340
                     /locus_tag="ACOIAS_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HGZ2703355.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1470 a          319 c          550 g         1322 t
ORIGIN      
        1 gtgttttgct tgttagtatt aatatttcaa ttgtcaatat tagtgtcttg cttgttagta
       61 ttagtatttc aattgtcaat attagtgttt tgcttgttag tattagtatt tcaattgtca
      121 atattagtgt tttgcttgtt agtattagta tttcaattgt caatattaat gttttgcttg
      181 ttagtattag tatttcaatt gtcaatatta gtgttttgct tgttagtatt aatatttcaa
      241 ttgtcaatat tagtgttttg cttgttagta tcagtgtttc aattgtcaat attagtattt
      301 taattgttga ctctagttat tgctgatata gaaaatattt aatttatttc aattaattag
      361 attaaagtag ggtagcatct aaaatagcta acatgtttaa gttgaaatac gtttaatata
      421 ttaatttttc aaagcattaa tatattaaag ttataatcaa aattgaagtt ataatctgcc
      481 aaatctatag cgtattaagt gcgttatata acttaatctt ttatataaag agttaatttt
      541 tttaatttgt aaagcagagt acgtttaaaa ggtactattt agataatgat gtactatcta
      601 attttaaaat atctacaata gggttctctt gaagacttgc tattgcttct gcaacagcat
      661 aaacatcttc atttaatgcc tctgctttta catttgcaaa tgatttactt ttaacaactg
      721 ccttaccctt atcatcaaaa ccagcatcca attttagttt tatactagat aaattttttg
      781 ttgaaacaac ttccataaaa ttacctccta aaaatttaat ttcaaagatt tgtcgacttt
      841 ctttgttagc tataatacta tcatctaaga taatatcgga gttaaacagt aatttctaaa
      901 gattttttgt gtaaattttt ataggaaaaa ttttactttt ttgctgatga aaaagtttac
      961 aagttatttg tgaagatatg ttcttagtgt tttgaaatag tatttgtata tattcatgct
     1021 tagttgagat agttttgtat ggattttcat atgtgtagag ttaaatgtta ggtactaaag
     1081 tttatgttaa attaaatgtt aaagcttatg ttaaaaattt tattaaattt aatctgtaaa
     1141 taaaaaagtt aaaagtccaa tattaacatt ttacttttaa accattaaaa tctttattaa
     1201 agctaacaaa gttataagtt atgagaaatt ttattaaagt taatacaaaa attaatttta
     1261 aagctaagaa attttttatt agattcaata aaactaataa atatagttta gtcaataatg
     1321 tattaaataa aaactttgtt aagtctataa aaatatctta aaggaggtgt aacttatgaa
     1381 gataaaagtg gaggatatac cagttcaatt tcatgcaatg gtagaaataa ttggaattga
     1441 taagtttata gaggttgcca agttgtatgg aggaacaaat acttatatac ctacgtataa
     1501 ggggcttttt agatatgcta gaaataggga tatagtaaaa caatttaatg gagtcaacca
     1561 caatgaattg gctattagat ataatatgtg tgtaagcaat attaagcgaa tactcaatga
     1621 aaataatgtt taaatataat aaaataatgt ttaaacataa tgaaaatagc cttgttaaac
     1681 gtaagagtaa agacaaagaa gatgttttat aaagtttttt tagacaaatt aactaaattt
     1741 actacttatt aatttaaata cattgtatat tctataaaaa aatttgttaa atactggaga
     1801 tatattgaga atacaaagaa aatggtcgaa aatatttgtt ggctaaaaaa tagataaaat
     1861 gatacaattg cttgtataaa ggggaaaaat aaataaataa actatataaa taaagaaata
     1921 gcaattaata tgacataata tatttataaa attaagaata taaatagata ttttaaggta
     1981 ttagttgtta aattaggaaa tattaaaaaa aatatatatt tatatttaaa ctattttgtt
     2041 atttatataa aaatacttat ctagttacta gcaattatta gtagaaatgg gggaaataga
     2101 gatgaataag ggattttaca aattaaattc agtggtattg aactttgata ttaagaatga
     2161 tgttaacaag ataagattta atcattatat aatgagaaaa gaaaacatac aaagacttca
     2221 aggaactctg ccaaacggac aattttatat gacagtgaga agagctgcta cagatttaca
     2281 actttctatt tctactataa gtaggcttgt aaaagagttt ttggatttag ggataataag
     2341 attagtgtct aagggtgtaa aaggtaatcg ttctgtatat agttacgtaa gtatagagtt
     2401 taatggaagt ttatttgatg atagcaaata tatggaactt gactgtgtat gtaatgatga
     2461 atttattgat aatagtggca tagagtttga ttgtgatagc aaagataatt cttttaatgg
     2521 agatataaat gtagaaactg tgcattgcat taatgagtat ttgtctgatg atgatgtaga
     2581 gcataattat aacaatttcc ataataaaga tactataaag gataaagcca tttctaatat
     2641 tagcactatc aaaaaatata ttgatgaatc ttttgataaa aataaatcag aaaattctaa
     2701 cttacaacag gagtcaataa atttcaactt taatgataca tgcaatgaaa tagataaaag
     2761 aacgaataat agtatagaaa ataatcaaaa taaaaggcct atgagattga gtgacatcag
     2821 tcttttaagt gataaaggta tgacaaataa aaagaattac tttaatcata atcatggtat
     2881 acaaaaaggg actaaatctg taacaaagaa aaaagaatcc ttaaataaat ttaataaaaa
     2941 aaatatacag caaagcatta taaatatgct aaataaaaaa tcaggtaaga attttaaaat
     3001 cgattctcca ataagcataa aacttatcaa tgaaaggcta aaggaaggct atgtcttaga
     3061 agatttctac aaagttattg aggtaaaagt ggctaagtgg aaaaatacca taatggaaat
     3121 gtatataaga ccagaaactt tgtttagcta taagtttgaa tcctatgtaa atgaaaacat
     3181 atctatgaaa agtcaaagta atgttcaccc atgtaaagaa ggcaatagta atgatagcag
     3241 taattatata cattctgatt atacaacata tgatggcagt gtgcgtgaga gaaaaaaata
     3301 tgagaatgta agatactgga atcctatatg tgaaatttaa ttagtttaag aatgtattta
     3361 tgataaataa gatattaata gagaatagtg agctatagca aatgatatat caatagttgg
     3421 tagtaagtta tattaagcaa gatattacta tcaggtagtg aattatattg aacgatatat
     3481 caataattgg tagtaaatta tattaagtaa gatattacta tcaggtagtg aattatattg
     3541 aacgatatat taataattgg cagtaaatta tattaaataa gatattaata tcgagtaatg
     3601 aattatattg aacgatatat caataattgg cagtaagtta tatcaaataa gatattaata
     3661 t
//
LOCUS       DBGJFU010000222         3444 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.226, whole genome shotgun sequence.
ACCESSION   DBGJFU010000222 DBGJFU010000000
VERSION     DBGJFU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.226"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(102..1669)
                     /locus_tag="ACOIAS_003690"
                     /pseudo
     CDS             complement(102..1669)
                     /locus_tag="ACOIAS_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(1662..2187)
                     /locus_tag="ACOIAS_003691"
                     /pseudo
     CDS             complement(1662..2187)
                     /locus_tag="ACOIAS_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ia"
     gene            complement(2576..3322)
                     /gene="cdtR"
                     /locus_tag="ACOIAS_003692"
     CDS             complement(2576..3322)
                     /gene="cdtR"
                     /locus_tag="ACOIAS_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HGZ2703356.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         1145 a          451 c          375 g         1473 t
ORIGIN      
        1 agataatatt taaataaaaa ataccttata aagttaattt tttaatactt tataaggtaa
       61 atacatattg tattgtgggg ataaatttaa atcatttgat actaatcaac actaagaacc
      121 aataactctc tatcatcttg agtaattgta tatattctta atttcttgta tgtcataata
      181 ttttctccac ctgtaaaata actcctaaga ttaacacaat cagttttagg ttgatttgta
      241 tctcctaaat aattacgcaa ttctttatct ttagtttcaa tatctttaaa tccaaaatat
      301 tgtaaagtag cttcaactct atatttttgg acataatcga gagcttcttt attagtttat
      361 gttggtgtaa aatacatacc atttatataa gcctttattg tacttggatt aaatttaaaa
      421 tctgcagaat atattttatg tgtaactatt atttcttgaa cagttggaat tttaacttct
      481 ggttcatcaa gtatttcagg agtactattt agctctgtaa tagataagtt atctaagtat
      541 attgtatcac tatcaattaa tgctatattt atattagaag aatctttttc agtagtttca
      601 aattcataac taaattttgt atatccttgt tctagtacca aataatccgt tttttcttct
      661 tttactttta tatttacaat tattgaatta tatgttaaag gattttttga attttactaa
      721 aaacataacg tttataaggt tttagttcag acataggtat tttaatcttc gtctcaccat
      781 ttaatctatt tgctgagcct tttaaaccat cttgatttgt agtattaaca ttactccata
      841 tactagggta attattgtaa tcatcaaaat tagtaaagta agttggtgtt tttataagta
      901 tattcattcc tctttcaagt ttaacattac atatttgttt atcagacaaa gtttttaaac
      961 tgtctttaat cttattgcct atattatcat caaatatgag ttcaacacaa ctttcatcta
     1021 ttggtatatt attaaaatat aacaaaccat cttttttagt agtgccaaaa gttttttcaa
     1081 ttgcttctcc aattgtaagt tcaggtgttt tatcttctgg gacctgtaaa cttttagcag
     1141 taattcttct tttgtaagat tcattttctg tatctaatat aatagataca gaaatactgt
     1201 caatttgacc tatatcgtct taccaactat ttcattctgt tactatttgt ccagaaccat
     1261 tttttgtacc aaaatttcca cttacttgtg ttgtttctaa tttaatttgc tttccatcat
     1321 ctaatttttt taattaatta taatttatag gaatcagtct agaactaaat tgatacattg
     1381 tgttaagagc cataggtgaa aatccttttt tgagataagt atttccagga gatagattat
     1441 tgccaatttg attttcttgt gctttgatag ttgataatgc atctccatct aacactaaat
     1501 ttgttgttgg tgtcactttg tacataggtg cagtacctgt gttgtagtat ctaatatttg
     1561 catttatata tgcagattct cctttgttta tacttaatcc agtatttcat gattatccat
     1621 tgctattttg aacagcagtt gaattatctg ttgtatggta ataatttgtc ttgtatagtt
     1681 tctttaaact cattaaattt ttctaatgaa atttcgtttt gattttttgt tcttattact
     1741 ttattaaatg caaatttttc tggagattca aaataatata catacatagg tttatctatt
     1801 ttattttttg atatagcatt tcgaagttct ctatattctt tttctcaaga atttgcttct
     1861 atttgataat cataaaaata atttcttgtc tgagaatatt tgcttatctc tatagaatct
     1921 tttttatatg atcctaatac ttctttttca gatttttcaa gtttttgatc ttttctttct
     1981 cattctttag ccttttcttt atctttaaaa aaatccttct gatctttcta taggagcctt
     2041 gtaagtagta ttacaaactt ttccactata gctttgtaaa tcttgtgcat aaatgttatt
     2101 aggtaacaaa ctacctaaag ttagatataa tatcaacaat atagatatac aattactaat
     2161 acttttatgt ttcctaaatt ttttcattta ttctccctac aaatattagt ttagttaact
     2221 tttgaatttt aatttattta gattatataa tctaaataag aattttttat ataaatttca
     2281 taaaatatgt aggtatatat cataaaaata tgtatttata tgatatgtca gtacaaaata
     2341 acaactgaat attttaaaga gactatttcg tacttttatg atttaatgag ctatcttatc
     2401 gaatcataat ctaggaacaa ttgttataat atctaatagt agttatatat gatatagtca
     2461 gtatgtgttt tttcacaata tgattattat atagaggatt cttagggatt actctttttg
     2521 tataaaaaag agatggttca aatttttatc ttgagacatc tcctttttct atttattatg
     2581 ttttaataat attctttaaa atatttctat atgtattaga tatacgacat atatggccat
     2641 tactcatttt aatattattt ttctttaggt cgagagaaac aatatgatat ttattaacta
     2701 caaaagattt atgtacacgg ataaagcatg catctaaatc tggtaaagac tttaaacttt
     2761 tatagaattc aaatattcta gaagtagtca ctaattttat tttatgtgga atagtgcttg
     2821 tttcaaaata tataacttca tcaaatgaaa ttttccatag tgagaaatct gaatagatag
     2881 taaaaaaatt agagcgataa tttttttctt taatgtatct tttgtgagca attttaatac
     2941 aagcttttat tttactttca gcaatttcca tatcatgttt tagtataaag tccataactt
     3001 ctaacctata atcgaagatc cctaaaatca tttctatatg atttgtaaga aatatgattg
     3061 gactaatata atctgattct ctaatttttt gtgctatcca taatccatca cataattcat
     3121 tacttatctt atattttgcg tctacaacta taaaatatat tcttacttta tttttttcag
     3181 taagctcttt taataacaaa tttgcattat gatataatct tacaacatca ttatcaaaac
     3241 cttcttttat taaaatatta tttatttttt cttttagttt cattccaaaa caaacatcat
     3301 tatcaaagat taatatatcc actaaatacc ctcctataaa aaattcaaaa gtaaaaaaac
     3361 aaaagtattt tatagaaaat acataaaata acactaggag ataaataaga ttaaggatag
     3421 ttagaattaa ggtatattta tgct
//
LOCUS       DBGJFU010000223         3429 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.227, whole genome shotgun sequence.
ACCESSION   DBGJFU010000223 DBGJFU010000000
VERSION     DBGJFU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.227"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..749)
                     /locus_tag="ACOIAS_003693"
     CDS             complement(<1..749)
                     /locus_tag="ACOIAS_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HGZ2703357.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKE"
     gene            complement(789..1136)
                     /locus_tag="ACOIAS_003694"
     CDS             complement(789..1136)
                     /locus_tag="ACOIAS_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703358.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1354..2310)
                     /locus_tag="ACOIAS_003695"
     CDS             complement(1354..2310)
                     /locus_tag="ACOIAS_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGZ2703359.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2753..3121
                     /locus_tag="ACOIAS_003696"
     CDS             2753..3121
                     /locus_tag="ACOIAS_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HGZ2703360.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         1261 a          436 c          430 g         1302 t
ORIGIN      
        1 atttcttttt tacttatatt tagtatagtt aatatttgtt taagatatat tattatatct
       61 tctggagttc tatcaaattt gagttcaaaa ttttcatctt caaagtaggg aatataatat
      121 aaaggtgttt tgagagcagg taatttatta tataattctt caagaatatc gatattttta
      181 ggggagttaa agaaaaagca tttacataca ttcattaaat ccacagcatc tgaaagtgag
      241 ttatgtttaa tatctccttc tacagaaaaa atttttttca tattcagtaa agatatttgc
      301 ttagataaga ttctattatt gtatgtaata tgttgagata taaaaggttg aatgtcaatc
      361 atcatattaa ataactctaa gcatttacct gtatatttat acactctaaa tgaagttaga
      421 aggccagtta tatcaaaatc accccaatta tatattgtaa catcatcaaa tttacaaaac
      481 catttataaa aatcatccat aacatataaa agattaggag cattattgat atctagttgg
      541 ttcaatttgg ttaaattttt acatttattt gaaagtattt tattgtattt tggcttaact
      601 aaagagtaat aattatctat tgtattaaat tcgttatcaa ctaaaactcc accaatagaa
      661 attaattccg caccattata ttcttttggt ttatttttat tatcatctag tatattaaat
      721 tcaaagtcta taaataactt gtatttcata aatctaaagc acatcctcag gtaaattatt
      781 tattgagcct aagcatcata agaatcaata gatataatga ctctaaacat caaaggttca
      841 ttacaacttt tatttatttc ataatttaat tcaaataaat cttttaagat ataatttata
      901 agtattcttt cccaacccat ttttaaatta actttagagt ctattaatat attttctatc
      961 tttttaaata tttctatatt tgctggtatt atactgagct caatattact tttaaacggg
     1021 ttagactgta aatctgttat aagtatattt tttaattcct ctgtaagttc agaaattaaa
     1081 ctatcaccta tgtatttttt tatgatttcc tctctaattt cttttaaatt tagcaatatt
     1141 agtacctgct acttacatag tagcttttcc tttcctttta atactattat tcatttttga
     1201 atcttattta gatatatttt aagattaaaa gtctttagct atataatttt ttacccattt
     1261 ttaacttata ttaacatatt acaggcttat atctccaccc tgtttacatt gaggatggag
     1321 tatattgcct attttgataa aaatgatagg ttgctatttg cctattcctt tttctgataa
     1381 tttttctaac atatctttgg tcattttatc taaatcataa ctaaacttaa agttccattc
     1441 ttttactgca ctactactgt ctagagagtt aggccaagaa tcagctatac tttgtcttac
     1501 agggtctaca tcatattcta tcacaaaatc aggaatatat ttttttatag aatcagctat
     1561 ttcttcagga gaaaagctca tagcagtaat attaaaagca tttctatgga tgagtttatc
     1621 agctggagct tcgattaaat ccactattga ttgaagtgca tcaggcatat acatcatatc
     1681 catttttgta ccttctgcta tgtaagattt atatctctta ttttttaatg cctcataata
     1741 tatatcaact gcataatctg tagttcctcc accaggaggc gtaacataag aaataagacc
     1801 tggaaatcta acacctctag tgtctactcc aaattttgag tgataataat cacatagtaa
     1861 ttctccagaa actttagtca caccatacat agtattaggt ctttgtaatg tatcttgagg
     1921 agtcatattt tttggagaat tttcaccaaa agcagcaatt gaactaggtg taaaaaattt
     1981 acaatccaat tctttggcta tttcaagacc atttattaat ccattcatat ttaagttcca
     2041 agcttctaga ggtttacttt cagcaactgc agaaagtaaa gatgctagat gaattatagt
     2101 atctacatca tatttttttg caatttcaaa aaaacgattc ttatctgtaa catctaatat
     2161 ctcaaatata ccagattcac atactggatt accttctttt actctccttg aactagcgat
     2221 tacattttgt tctccatact catttctaag ctttattgtt aattctgaac ctatttgtcc
     2281 taatgcacct gttataagta tttttttcaa ttttacaacc tcctaagggt ttaatttatg
     2341 tagtactact tatttttatt ttagcaaatc ttttgtttaa atacaaagaa ttaaagataa
     2401 taatatacga cttaattgaa tgaagtgcag taagtgcgta aaaatttaaa gctaatatat
     2461 aatggtctat tgtataacaa taaaagagta gattaaatta ttatatatag attgtgtgac
     2521 taagtcacag aaaatagagc taatttaata tataataaaa gcatgtcaag taaagcttta
     2581 aattaaagta actttatata aaaagtaata taaattgata aaattataaa tttatttata
     2641 gtgaatatag gttggatttt atagatacaa aaatagtata tatgatatgt agaccattag
     2701 catgtttaaa gtagcaaaac atataaaata aaatttgaaa ggaagtataa aaatgacaag
     2761 accatcaata tatcatagcc aaggatacac ttgtgcagag gcattaataa agtcttataa
     2821 tgaggaacac aacacagata taccaatctc aataggaagt ggtatggggg ttggaatgaa
     2881 tgttggaagt gtttgtggag ctgtaaatgc tgctgttgtt ataatagggt atttaaatgg
     2941 gcgaaatagt aatttagatg aaaatatagc aaggaagtat tcaagagagc tattgaatag
     3001 agtaagaaat aaatacagct cagaaatatg tgtatctctt aaaaaaaata aagtaacttg
     3061 tgaagaaata ataaatttca catatgatgc tctaaatgat atgctaaaaa atcaaaaata
     3121 attctaagaa acaagtattt tacattcgat agaatacttg tttttttatg atatttttaa
     3181 taagtattag tttattataa tttttaaatc aattttatta tattttaatt gaaataatct
     3241 actttgaaaa ttactaactg acaaaagaat atgattatag tatagaaaaa atgtaaatta
     3301 tttaatatta agtgagagta tatgcttacc tatataaaat cgtataaatt aatggctaaa
     3361 tttataaatt ataaagaaat atataggaaa ataaaagaat taattagtat aatataaagt
     3421 taaaaagtg
//
LOCUS       DBGJFU010000224         3408 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.228, whole genome shotgun sequence.
ACCESSION   DBGJFU010000224 DBGJFU010000000
VERSION     DBGJFU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.228"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            53..400
                     /locus_tag="ACOIAS_003697"
     CDS             53..400
                     /locus_tag="ACOIAS_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGZ2703361.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(550..1599)
                     /locus_tag="ACOIAS_003698"
     CDS             complement(550..1599)
                     /locus_tag="ACOIAS_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2703362.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            1985..2719
                     /locus_tag="ACOIAS_003699"
     CDS             1985..2719
                     /locus_tag="ACOIAS_003699"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2703363.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNALMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(2862..3353)
                     /locus_tag="ACOIAS_003700"
     CDS             complement(2862..3353)
                     /locus_tag="ACOIAS_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HGZ2703364.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT         1201 a          465 c          479 g         1263 t
ORIGIN      
        1 atattggtat aataatagta aatctataat atagaaaaga ggtatttcta gtatgcatga
       61 aaataaaaaa aatctattag acggaagctt agctcaatat aatgatacag cagttccaaa
      121 aataccagta tttgctggta acgatataac ttcagaagtt tattatttta aacctaatca
      181 agttcttaat gctcatagac accctaatgg tgaacaaata tttgtgttcc taaaaggaga
      241 aggtaaaatg aaacttggtg agcatgaatg tgatgtaaaa aacggagata ctgtatttgt
      301 accaacagga gagtggcatg aaattactaa tggaagcaat gaagaaatgg tagccgttca
      361 aattacaaaa attaatgctg gagcagaata cagaggatag atatataagt acagttagta
      421 ctttaatatc tattttatta tataaacttt aatctcaaat ataaaaagag tcttttaaaa
      481 ggctcttttt atatttttgt taattttcat tataaaaaca agaaaatatc tagctaaaac
      541 taatatattt tatttaatat tttttatagc attgtttgca aactcagtat ttacgatatc
      601 tttataaggt gcttccttat ctagttcttt agcttctttc ataacagtca tcaatttctt
      661 caaaccttcc tcttttaata caggattttg agaccatgca tctattgatt tatatctatc
      721 tacaactgtt gttaaatctt ctaaagatat atcttcaaag aaaggttgca tagccttagc
      781 tacttctttg ctagatgctt gttgaatcca taattgacct ttataaacag catttgtaaa
      841 tctttgaatt aaatctttat tgtttttcat gtatgatctc gtagtagagt atgctgtaaa
      901 aggtatttct ccagaatctt ttcctataga tgtaactata tatccactac cttctttttc
      961 cattgctgtt gctgtaggtt caaatgctgt tgtaaagtca ccctctccac ctgcaaaagc
     1021 tccagccatt acaccaaatt gtacatcagt tcttacatta acattgcctg cttttagatt
     1081 ttctccaata gtaagacctt tttcttttag aacatattca aaagtcatta gtggaactcc
     1141 accttttcta ccacctaata tttcggttcc ttttaaatca ttgtaagaga aattaggcat
     1201 ttttttccta gcaactagaa aactaccatc tctttttgtc aattgagcaa agttcactgc
     1261 ataatcgttt ttgttttgat tatatacata tatagatgct tcaggaccca ttaaacctac
     1321 gtttgcttca cctgaaatca aagctgccat agttttatct gcaccttggg tatttataag
     1381 gtcaatcttt ataccttcat cttcaaagaa tcctttcgta atagcagcat actgtggagc
     1441 ataaaaaaca gagtgagtaa cttctgctat agttacttta tccagatttt tagacgaaga
     1501 agattcatct tttttgtcat tagagttaaa atcacatcca gttagacata ttgtacttac
     1561 aatgatagag cttgctattg cgactatttt tttaaacata ataaatacct cccataagac
     1621 atacgaatag tatatagtat taaatattta ttgtagttgt tacaaaaatg tatctatact
     1681 tataatattt ctacaaaaac ttaataataa ttttcagttt tatgtttagt tttttcagta
     1741 atgtgtaata ttggataaat tatattgatt atttataaac taagtaataa aattaaaata
     1801 atatcacttt tttgtctatt atatataagg aaaaattttt taataatata aatcatattc
     1861 atttatatta ttaaaaaatt tgtttataga aacttttatt attttatata aaagtgataa
     1921 aaacttatat tttgtattta atagagtgtt gctaagtgaa tatatttaaa atggaggaaa
     1981 ctttatgaaa caaaataaat atgatgatga tagatttttt aataaataca gtaaaatgga
     2041 acgttctaca aatggattag caggtgctgg agagtggcat gttttaaaaa aaatgttacc
     2101 tgatttcaaa ggaaaaagag ttttagattt aggatgtgga tttggatggc attgtcaata
     2161 tgcagttgag aatggagcaa tttctgctgt tggagttgat atttcagaaa aaatgttaaa
     2221 agaagctaga aacaaaacgc aacttgataa tataaattac atatgtatgc ctattgaaga
     2281 tattaatttt ccaaaagata gttttgatgt agttattagt tccttggcat ttcattatat
     2341 tcaatcattt gaagatatat gtaagaatat aagtaattgt ctttcaaata aaggtaattt
     2401 tgtattttct gttgaacatc ctatatttac tgcatatgga agtcaagagt ggtactgtga
     2461 taaagatgga aataatctac actggccagt agatagatat tttgaagaag gagtacgtag
     2521 gtcaaatttt ctaggagaag aggttataaa atatcataaa acattaacaa cttatttaaa
     2581 tgcgttaatg aaaacaggat ttgaaatatt agaaattatt gaacctcaac cagaagaaaa
     2641 actgttagat acaattcctg atatgaaaga tgagctacgt agacctatga tgttacttgt
     2701 atctgcaaaa aaaagataag taaatataaa taatcattat tgtagaaaaa aagttgtaga
     2761 aaataattac actaatggag atgattatac cagtggtatt aattacacca gtagtaagga
     2821 ggcaaaagca ttcgttgctt ttgccatttt ttaagttgca attatacttc tacgtagtat
     2881 atttcgtttc cattttcttc tataagcttt ttcatatctt caaattttaa aaatacagta
     2941 gcagtattat catttggatg tatacctatt ttatccattt ttaataaatc cttatcaaat
     3001 acaactttta caacagcctc tttatcattt aatataccaa aaggagtcac agctccttta
     3061 gtaagtttca agtatttttg aagtctttct tctgatgcaa aactaagagg tttactatca
     3121 atttgttgac gaatatcttt taaatctgct tttttgtctt tacctaatac aactaaaaaa
     3181 tgttcttttc ctttagcatc acgaagaaat aggtttttta caacaaattc catatgtaat
     3241 gtttttaatt cttccatttc ttcaattgta taaactggtg gatgatttac agcctcataa
     3301 ggtatgctta actcatctaa ttttttatat acttcctgtt gttgattgtt catgatttcc
     3361 tcccattaga aatttatttt atatataaat aatattatca cagaaaac
//
LOCUS       DBGJFU010000225         3391 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.229, whole genome shotgun sequence.
ACCESSION   DBGJFU010000225 DBGJFU010000000
VERSION     DBGJFU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.229"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            2..1018
                     /locus_tag="ACOIAS_003701"
     CDS             2..1018
                     /locus_tag="ACOIAS_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGZ2703365.1"
                     /translation="MFIVYFVSIELKDRSLVNNQYKGKIKSANLSVDYEINQVMKDID
                     KLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWIENG
                     KLYETDWNPINKKRFFYTWQNAILNELIIDVANPLDVNVLNIGSNFVHLEEYQQNWGE
                     IIDFVQSKFDGFVTYRTNWWYTKKDDLSSKLFYEQKLNNNFFDKLDFISIAAYFELSN
                     KPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPW
                     NHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIK
                     SWYE"
     gene            1072..2196
                     /gene="wecB"
                     /locus_tag="ACOIAS_003702"
     CDS             1072..2196
                     /gene="wecB"
                     /locus_tag="ACOIAS_003702"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGZ2703366.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGMQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2274..3248
                     /locus_tag="ACOIAS_003703"
     CDS             2274..3248
                     /locus_tag="ACOIAS_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGZ2703367.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1418 a          333 c          595 g         1045 t
ORIGIN      
        1 tatatttata gtttactttg taagtattga gttaaaagat aggagtctag taaataatca
       61 atataaaggt aagattaaat cggccaattt atctgtagat tatgaaataa atcaagttat
      121 gaaggatata gataaattgg gattgaatac agtaaatgta cctataatta taaatgtaga
      181 gtccattaat tctgatatta tgtcaataga taataatagt aaagaaaagg caattaagtt
      241 aataaaaaaa ttaaataaaa aaggtatttc aacaatatta gaggcatatc catggattga
      301 aaatggaaaa ttgtatgaga ctgactggaa ccctataaat aaaaaaagat ttttttatac
      361 ttggcaaaat gccatattaa atgaacttat aattgatgta gcaaatccac tggatgtaaa
      421 tgttttaaat attggttcta attttgtaca tttggaagaa tatcaacaaa attggggtga
      481 aataatagat tttgtacaat ccaaatttga cggttttgta acgtacagaa ctaattggtg
      541 gtatacaaaa aaagatgatt taagtagtaa gttgttttat gaacaaaagc tgaacaataa
      601 tttttttgat aagttagatt ttatatctat agcagcatat tttgagttaa gtaataaacc
      661 tgttaataca gtggatgaat taatttctgc cttacactca tctactgtaa acaatagagg
      721 acaaaatata aaacaagaaa tatataacct ttataaagct catagaaaac ctatattttt
      781 tggagaatta ggtttttcaa atagagaaag tgcaagttct cagccgtgga atcatactcc
      841 ttcaaaagct gtaaatggcg aagaacaagc aaggtgtttt gaggcatata agaaagtttt
      901 tgaaaatgaa gactggataa atggtttttc tgtgttttgt gtaggtaaaa tagatgacga
      961 aaaaaatttt tacccatcaa aagagagtat aaaagttatt aagtcttggt atgaataatt
     1021 ttgaaagtgt agaatttata tagattaaat agatgattag gagagacata aatgaatgac
     1081 atcaaggtta tgactgtatt tggaactaga cctgaagcta tcaaagtagc acctttaata
     1141 aaggaacttg aaaaaagaga gaatataaaa agtattgttt gcgttacagc tcaacataga
     1201 gaaatgcttg accaagttat agaaaccttt aatataaatg tagactatga tttggatata
     1261 atggaaaaag gacaaagcct gaatgatata acatgtaaga ttttaaataa acttcccttg
     1321 attttaaaca aggaaaatcc aaatataata ttggtacatg gagatacaac cacaactcta
     1381 gctacaagtt tgactgcatt ttataataaa actcttgttg gtcatataga agctgggctt
     1441 agaacctatg ataaatattc tccttttcca gaggagttaa atagacaact tacaggtatt
     1501 attgctgata tgcactttgc tcctactaat ttggctagaa aaaacttgat ttctgaagga
     1561 aaacctaata ataatatatt tgtaactggt aatactgcta ttgatgcttt aaagatgaca
     1621 ataaaagaaa actataatca tcctataatt gatgagattg gtaacgatag gatgatttta
     1681 ctaacctctc acagaagaga aaatttgggt aaacctatga aaaatatatt tagagctata
     1741 aagcgaatag tagatgactt tgaagatgta cagatagtat atcccataca tttaaatcct
     1801 aaaatacgaa ctatagctga tgagatattt ggaaaatttc ctgaaaaaat tcatattata
     1861 gagcctttag atgtagctga ttttcataac tttttaaata aatcttatat gataatgact
     1921 gatagtggag gaatgcagga agaagctcca tcattaggta agccagtcct tgtgttgcgt
     1981 gataaaacag aaagaacaga aggaattgaa gcaaagacac ttaaattagt aggtactaat
     2041 gaagatagaa tatataattc agtgagcgat ttattaataa ataaagataa ctatgttcag
     2101 atgagtaaag catctaatcc atatggagat ggaaatgcaa gcaaatatat agtggatata
     2161 ataattaaaa aatttaattg caaatattta aattagataa gacctaaaac attaactaat
     2221 tgatataaga tttatgataa tattaaggaa tacaattttt aagggggagt aatatggcta
     2281 gaaaattaat aaaaaatttg ggtaaaagta aaagtgtaaa gagagtgaaa cttttattta
     2341 agaagatttt tattactgta ttcatagtag caagtatagt tgctattttt aatataacga
     2401 aatattttga ggaattatac aaagtaaggg atttaaaaag tactaaaatt gaatactata
     2461 tggatgtggc tgatgaagca ggagatggaa aggtccaatt aagttggaag gccttacttg
     2521 ctatagacat ggtaatacat gatgaagatt taagtaatat aaaaaagaaa gacacattgg
     2581 atatagggga aaagtttata gtagaagata aaaatgataa aggcgaaaag gtgtataagg
     2641 taaaaaagtt taataaggta ttaagcgaat tgaaatttga ctcttctcaa aaaagtagag
     2701 caagaaaata catgaaagat ttagaataca cataccttgg aaataaacaa ttagatagta
     2761 gtgatgaaaa aattaaattt ataaagaagt tagaagactc agctataaga gaatatattg
     2821 attatggaat attgccctct ataacaattg gacaagctat attagaatct ggttggggaa
     2881 attctaaact tacaaaacag agtaataatt tatttggtat aaaagcagat aaagcatgga
     2941 aaggaaaaag tgtagaaatt tcaacttcag agcattataa tgaaaaaatt gtagctagtt
     3001 ttaggtcgta taattcatta caagattctg tcaaagacca cagtttattt ttaattaata
     3061 ataaaagata tagaaaacat ggattgtttg aggcaaaaga ttatattagt caagctcaat
     3121 cattagaaaa tgcaggatat agtacagcag aagataaaaa aggaaatcgt atatatgcag
     3181 agctattaat agatgttatt aggagttata atttgcaatt gatagataat aaagttgaga
     3241 caaaataatt gttaacttta tatgaggaga taacaagata agattctttt ctacaaaaag
     3301 aatagaaaaa ttgtaaaaaa aactaaaaaa atgtattgac ttgttattta aaaggtgata
     3361 ttattattac tgtactcgaa agagaacaaa a
//
LOCUS       DBGJFU010000226         3380 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.230, whole genome shotgun sequence.
ACCESSION   DBGJFU010000226 DBGJFU010000000
VERSION     DBGJFU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.230"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            416..3058
                     /gene="adhE"
                     /locus_tag="ACOIAS_003704"
                     /gene_synonym="adhC"
     CDS             416..3058
                     /gene="adhE"
                     /locus_tag="ACOIAS_003704"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGZ2703368.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1321 a          420 c          629 g         1010 t
ORIGIN      
        1 aaattgcata caagtcaaaa ataagtgtta ttttataaaa atacgacaaa aattttattt
       61 actacttata caaataacat gcaaataaca tataaaattt aataagtaaa aaaaatgaaa
      121 gatataaatt atcattttag tattttattt agtcgaagta aattattcaa atgaaatata
      181 tctcatttag ataaaaaatt ttagtaaaag taaaaaaaat aatatatttg ttaaaaaata
      241 aattgacaaa atattcagaa aaaaacttct tgacctaaga gagtaaaata aataaaatct
      301 aaggtataga gatgaaatct ataaaattat tttaagtata agttaaaaat atagatgatg
      361 catttaagat taataaaaat tagagatgca taattatttt agggaggttt ttagtatgga
      421 gaaaaaagaa aaggtagtag aaaaatcaaa tgttgaagta tgtagtcctg aaattgtcaa
      481 cagtgttgaa acattaagaa tgagattaga agaaattaga ttggcacaaa aagaatttgc
      541 aacatttacg caagaacagg tagataagat tttcttagct gcttcaactg cagctaatca
      601 acaaagaata cctctagcaa aaatggcagt ggaagagact ggtatgggta ttgttgaaga
      661 taaggttata aaaaaccatt ttgcctcaga atatatctat aatgcttata aagatactaa
      721 aacttgtggt gtcatagaaa aagatgaagc atttggtttt acaagaattg ctgagccagt
      781 aggtgtttta tcagcagtta taccaacaac taatcctaca tcaactgcaa tattcaaatc
      841 cctaatagca ctaaaaacta gaaatggtat aattttctct cctcatccaa gagctaaaaa
      901 ctgtactata gaagcagcta gagttgtaca tgatgcagca gttaaggcag gagcaccaaa
      961 aggacttata ggttgggtag atgttccttc gattgaatta actaatgttg tcatggcaga
     1021 ggcagacctt atattagcta ctggtggacc tggaatggtt aaatctgcat actcttcagg
     1081 taaaccagct gtcggagttg ggcctggaaa tgtacctgca ataatagatg agagtgcaga
     1141 tataaaaatg gctgtaagtt caatcttagt atcaaaatca ttcgataatg gtatgatatg
     1201 tgcttccgag caagctgtaa tagtaccaga aaaaatatat gaagaagtta aaaaagaatt
     1261 taaatatcgt ggagcacatt tcctaaataa agaagaaact gaaaaagtag gaaaagtagt
     1321 aataattgat ggttcattaa atgccagaat agttggacaa ccagcacatg taatagccaa
     1381 aatggcggat gtagaagttc caaaaacagc tagaataata attggtgaag tagaatctgt
     1441 tgaattaaat gaaccttttg cacatgaaaa actttctcct gtacttgcaa tgtacaagtc
     1501 taaaagtttt gaagatgcag ttgctaaagc agaaaaatta gttgcagatg gaggctatgg
     1561 tcatacatct tcattatatg cagactctat aaatcatcca gagagagtag aaaagtttgt
     1621 aaatgctatg aagacttgta gagtattagt taatactcca tcatcacaag gtggtatagg
     1681 tgatttatat aactttaaat tggcaccgtc tcttactctt ggttgtggtt cttggggtgg
     1741 aaactcagta tctgaaaacg ttggagtaaa acatttgctt aatatcaaga cagtagctga
     1801 aaggagagag aatatgcttt ggttcagagc accagaaaag gtttatttca aaaaaggatg
     1861 tttaggtgta gcagcaagag aatttaagga tgttatggat aaaaagaaag catttatagt
     1921 aacagattct ttcctatata ataatggata tactaagaaa ttaactgatt tattagatga
     1981 gatgggaatt aagcatacta cattctttga tgtagctcca gacccaacat tagcttgtgc
     2041 aagagaagga gctaaagcta tggctgattt ccaaccagat ttaataatag ctgtaggtgg
     2101 aggaagtgca atggatgctg gtaagataat gtgggtaatg tatgagcatc cagaagtaga
     2161 tttccaagac ttagctatga gatttatgga tataagaaaa agagtatatg tatttcctaa
     2221 gatgggtgaa aaagcgtatt ttgctgctat cccaacttca gcaggaactg gttcagaagt
     2281 aactccattt gcagttataa ctgaccaaga ttcaggtgta aagtacccat tagcagatta
     2341 tgaattaatg ccaaacatgg ctataattga tgctgatatg atgatggaga tgccaccaag
     2401 attaacagca gcatcaggtg ttgatgcttt aacacatgca ttagaagcat atgtatctat
     2461 gttaagaact gaaccagctg acggtctagc actacaagca ggtaaaataa tatttgaata
     2521 tttacctcgt gcatataaaa atggaaagaa tgataaagaa gcaagagaaa aaatggcaat
     2581 ggcatctact atggcaggaa tgtcttttgc aaatgccttc ttaggaatat gtcactcttt
     2641 agcacataaa ttaggtgcat tccaccatgt tcaacacggt gttgcaaatg cattattaat
     2701 aaatgaagta atcaagttca attgtgcaga agctccaaat aaaatgggtg cattctcaca
     2761 atacagatat cctgattgca tacaaagata tgctgaattt gcttcttttg caggtattaa
     2821 aggaagtaca gaccaagaaa aagtagataa tcttataaaa gcaatagatg aactaaaggc
     2881 aaaagtaggt cttcctaaga ctataaaaga ggctggagta gaagaatcta aattcttaga
     2941 aagattggat gctatggttg aacaagcttt tgatgaccaa tgtacaggtg ctaatcctag
     3001 atacccattg atgagtgaat taaaagagat atatctaaaa gtttattatg gtaaataata
     3061 tctattgtgc aacataggtt ctattgggtt tgtatataaa ttattagatt tgaatataaa
     3121 tagagtttta ttagagacac gtagaagtaa ctataaataa tagattataa cttaaattct
     3181 aaatggaaat gataaaaagt cagtaattta atggtaattt cgaatatgtg tatttaatca
     3241 attaacttta aaattagtcg gaaaattttt ttattacata taattttaaa gcataatata
     3301 taaaaaactt tatgaaaaaa ttataaaaat attcagaaaa ttctattgac taatttattt
     3361 atcatgatat aataagttta
//
LOCUS       DBGJFU010000227         3373 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.231, whole genome shotgun sequence.
ACCESSION   DBGJFU010000227 DBGJFU010000000
VERSION     DBGJFU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.231"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(90..458)
                     /locus_tag="ACOIAS_003705"
     CDS             complement(90..458)
                     /locus_tag="ACOIAS_003705"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2703369.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFD"
     gene            complement(479..1558)
                     /locus_tag="ACOIAS_003706"
     CDS             complement(479..1558)
                     /locus_tag="ACOIAS_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HGZ2703370.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1612..2901)
                     /locus_tag="ACOIAS_003707"
     CDS             complement(1612..2901)
                     /locus_tag="ACOIAS_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGZ2703371.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(3064..3275)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1110 a          678 c          371 g         1214 t
ORIGIN      
        1 tatcctatct ctacatatag gtttccctat atgctgacta ttttagctac taactcatga
       61 ctaaagtcac gagtgtgcgt agctattttt taatcaaact catatccacc agataccaat
      121 attgaatcta attctatttt ttctcttaag tcttctggcc aatctataaa tggaagaatg
      181 gcagtagtgg ctggctttcc tattcttatc atagccacat tattaagtct ttttctttct
      241 acaatagcat ttgtacaatg agttgtacca agcatagcat attttattaa tgaatggtca
      301 atcttacttt catctataac tttttttaag gcattgtaaa caccagtttc aacatctttt
      361 gtagttgcca ccttaactga atgaattact tttaattccc catccagtat acaagcatct
      421 gtatttgttc ctccaacgtc aattcctaca cgatacatca aaatttcctc ctaaactttt
      481 attttccttg taattcttct actgttttat attccacatc atatccaaag tatccaggtc
      541 caactgtctc aattcctttt tctgttctcc atttttcatt acaaggaaaa gctactacca
      601 taacacgaga gccatatttt attctctctg tagttattgg aaatccagtt tctaaatcta
      661 atactgctat taaatctggt gtaatacaaa gtggtgtatc accttttttg gcaattaaat
      721 tctcattttg gaataatata gaatactctc catcatactc attaatccca gcaaaaaagg
      781 catgtcctct agtaaatcca cctttcaact ctctcttaat atcaattact tttccttcaa
      841 ataacttgta tccacgcaat tcttttagta tattctcaac agcacctttt gaatcagatt
      901 tattttcaag taaaaaccct cctatagttt cagctattgt aagagtatta cctatagcag
      961 cttttttaac ttgagctcca cttaaattat agtcactaat agaagaactt cctcccattt
     1021 ctacagttaa agttctagct aatctttctg accaaattcc atccacagga taaaggatgg
     1081 cagaattacc tttttcatca gacattacgg caggagatgc ctcatatcct tcaagataaa
     1141 aagttaccat ttgagcttca gggaaagctc tacccataga atctgcatct aagataggaa
     1201 gtcctttttt agcagcaact gctataggca taagtgagtt tatccctcct atttctatag
     1261 gaattataac atccactttt ctatttaata atttctccat tatttctaaa ggggtagtta
     1321 actcaacttc tgaaagtaat ttttctattg taattacagg agaacccata ccagatactg
     1381 gaactaccaa agcatcatca tcaagttcat ctaaacttat tacttttaca ggaccatact
     1441 ttttaatggc ttgttttgca accaatttac ctacatatgg gtcacctcca cctccagttc
     1501 caagcactgc tgcaccaact gctattttat ctacactttc ttctgttaaa aatctcatgt
     1561 tactttctcc tcatatttct aatttatttt atactttata aacaaatttc tttatactgc
     1621 tttgtctatt tcttgcttat tttttgttga acaaagcact tttccaataa tagattgtac
     1681 tacaaaccct gctataaaac catctaaagg tgctatagta gtaagagtta taaatccata
     1741 tgttgaaagg taagttatta atataccaat tatccaagac actattgaac gtttaactat
     1801 tggtttatat aatactcctt tatcaaagcc tttaagttct tgctttacta tgtaatattc
     1861 tgctgtatat acaccaccaa caggtgcaat tacaattcca agtaaattta aaaatcctat
     1921 aaaaccttca tatattccaa atacagccaa taatgttgct ataataccaa ggactatagt
     1981 taatactttc tcaggagcct tcttaagtac aagagctata cttaatgatg ctgaatatac
     2041 attacttgta tttgtagtcc attgtgctaa tgtaagtact attatacctg gtatagcaat
     2101 tcctaatgta ataaaaattc tcataatatc tgaagttccc atacatttta ctaatactat
     2161 cgaaactatt atcataaaag catttccaaa ttgtattccc aaaaagcttg aaagagcaca
     2221 gtctcttcta cttttagccc aacgacttat atcaggagat acaattgcac caactattat
     2281 tatactcatt gacagagaaa ctccacctgc aaatgtcata gtagatttca tattatcatc
     2341 aactgaaatt ccatttgctc taaaagctaa gaatatagtt agcatcataa gaactaataa
     2401 aaatggtact gaatacacac tcaacttctc cattgcctta tatccataaa ttgcagtact
     2461 catcatcaag agaccaccta ttaggcttaa aatctgcata cttacatcta aattaaatat
     2521 atcttttatt attatctgtg cattttgagc aaagaaacct acttgaactc caaaccaacc
     2581 taatagagaa acccctataa ctagagaaaa tatttttgct ccagcttctc caaatacata
     2641 ctttgttatc atagatgtag ataaacttgt agatgagcca accaaagagc atgtagtata
     2701 caaaattgca gaaaaaaatg aaccaacaat tactgacaat attgcttgtt tcattggcat
     2761 actctgtgcc aactcagcgc ctaaaataac agatgataaa tccacaccta ttgctactaa
     2821 aactgaaaac atagctaccc aaccacgttt ttgattctct ggaatctgtt ccagagcata
     2881 gttaattttt tcttcttcca ttttcatccc tccatttaaa tcgtaaaact actgacctta
     2941 gcatatttta caattttcta atttataaat ttattaacta tcatattttt ataattttca
     3001 tttaatatat agttttaaat tttctgaaat aagtttataa taaaaaacgc ctcttggaaa
     3061 aatccaagag acgaatataa atccgtgtta ccactcttat tagtaaaaat taattttact
     3121 cactcatttt cttaaggcga attaaaccac tattctaaca taattacaat agtattctcc
     3181 aaagtccttt tcacataaat tccaatatta gttctcagcc aaatctaact ctctgtaaaa
     3241 ttcctttatg ctactttctt cttctacgaa tatataatat tgatttgtag ttaatttatg
     3301 atttatatca taatatatat gcaatatttt gtcaacttaa aaagtcaatt acttgcattt
     3361 tttagtttta tat
//
LOCUS       DBGJFU010000228         3319 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.232, whole genome shotgun sequence.
ACCESSION   DBGJFU010000228 DBGJFU010000000
VERSION     DBGJFU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.232"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            94..849
                     /locus_tag="ACOIAS_003708"
     CDS             94..849
                     /locus_tag="ACOIAS_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703372.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            946..1983
                     /locus_tag="ACOIAS_003709"
     CDS             946..1983
                     /locus_tag="ACOIAS_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGZ2703373.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2792..>3319
                     /locus_tag="ACOIAS_003710"
     CDS             2792..>3319
                     /locus_tag="ACOIAS_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703374.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLR
                     IMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQM
                     IKSLGINIGSIKILGL"
BASE COUNT         1256 a          434 c          501 g         1128 t
ORIGIN      
        1 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
       61 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      121 agtttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      181 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      241 aatataatat ctgggcttat aaaagaatct tctggtcaaa ttactttaga tagtactact
      301 ataacaaatt tagctgagta taaaagaact caaatagtaa gtagagtttt tcaagaccca
      361 agccttggaa cttgtccttc tatgacggtt agagaaaatt tatctctagc acttaataaa
      421 gggaaattat tgaacttaaa aaaatgtctt cgtcataaaa cgaatgactt ggaacactta
      481 cttgaaggaa tttctttgga tttaaaaaaa tatttagata ttaaagtaca atatctttca
      541 gggggtcaaa gacaatcact atcactaatt atgtctagct tagcaagtcc taaggtctta
      601 ttactagacg aacatacagc agcacttgac ccaaaaacat caaatgaagt aatagagtta
      661 acagataaaa tcgtaagaga gaaaaatata actacactta tggttactca caacttgaaa
      721 cacgctcttc aatatggaga taggttgata atgcttcata agggtgaagt tgtattagat
      781 gtaaaagata aagaaaaaaa agatttaaca gttgaagaaa tcttagaaaa atttgaatat
      841 gcagtataag aaattaaaat cttagtttta atcagaaata tttataatct atcatatgaa
      901 tatatgaaca aatataatta ttaaatttta agggaggaaa ataaaatgat aaaaagtaaa
      961 aaaatattaa gtttaattat agcaggagta cttggtgtat caatgcttac tggatgttct
     1021 caaaatgatg gctccaatgc ttcaaatgaa aataaagaaa cagatagcaa aaaacaaaaa
     1081 aatataggta ttactcaatt agtggaacat ccatctctgg ataaagcaaa gaaaggattc
     1141 atcaaagcac ttgaagataa aggctataaa gatggagata atataaaaat agatttccaa
     1201 aatgcacaaa atgatatgcc tactacacaa agtattgcta gtaagtttgt atccgacaaa
     1261 aaagatttga tatatgctat atctactcca tctgcacaag cagcttataa tgctactaaa
     1321 gatattccaa taataatgac tgctgttaca gaccctgtag aagctggact tgttaaatct
     1381 cttgaaaaac caggtggaaa tgtttctggt acatctgatt atctttcaat tgataaaaca
     1441 ttagaattgg ttaaaacatt gactccaaaa gcaaagaaaa taggggttat atacaatact
     1501 agtgaagtta attcaaaaat ccaagttgat tctctacatg attatgctaa gaaaaataat
     1561 tatgaagtag ttgaaaaagg aatcagctct tcaagtgaag ttaaccaagc tatttctagt
     1621 ttagttggta aaatagatgt tttatatgtc cctactgaca atttaatagt ttcttctatg
     1681 ccaatagttt ctaaagttgc taatgaaaac aagattccta taatagcctc tgaagaaggt
     1741 tctgtatctt ctggtgcttt agcttgttgt ggaatagatt atgaaaaact aggttataag
     1801 gctggagaac ttgctattga agtattagaa ggaaaatctg ttggcgatat accagttact
     1861 acattagatg aaactgaaat aataattaac gaagatacac taaaagcact tgacatgcaa
     1921 aagttatcag cagataatat aaagtatata aagtcagatg aaaatgcaaa atctacaaaa
     1981 taaaacttat taatttcaaa aaataggtaa tatgaagtag gtaatatgaa aactttatat
     2041 aataagtact cttatccacc ctaacccttt gaaaattgtg cttattgtag caatttgaat
     2101 attgcatata aagttattca ttttccataa aagtcgtcta tattgacgac tgattatgaa
     2161 agttatataa ctttctaatc actacttgag ctgtccaaaa gaattttaat attctccaga
     2221 cagctttttt ttatgatatt tgattgatat acatgtctag ttattccata tttagtaaat
     2281 attttgataa aatttccaca ttcacttata acttaggata acgttttttt caaacaatac
     2341 attaatttaa aagtattctg agtatacatt atttagtatt ttaattaaat ttagcagttg
     2401 acttttatta ttgtataatt tataatttat aacaagtcga gaatacatat aattttctga
     2461 ctaattaaaa tattaaaagg gagagataat tatgaatact ttattaaaca atagaaaata
     2521 ttattattat tatagccact atttttaggg tttgtaccta cgttttaaca tagatacaga
     2581 ccccttaaga ctactaaaat tttgggattg tatctatgtt ggctatattg tttataaagt
     2641 tttcatttaa ctatataaga gccacataga ttaaactatg tggcttattc gtgtttttac
     2701 cacatagttt tgtacactat gtggtttttt tatacacaaa attagcaaat aattaaaatt
     2761 ataaaaatat tttcaaagga gtgttttatt tatgtcaggt attatatctg taatgacgca
     2821 aagtttaatt ttatcgatta tggcactggg ggtttatata acttacaaaa tcttagattt
     2881 tccagatatg tctgctgatg gtagctacac aatgggtgct tctattgtag cattttctct
     2941 tactaatggt atttctcctg tagtagcaac tttaatggct atcttatgtg gttgtactgc
     3001 tggtcttgtt acagggattc ttcacattaa gtttaagata tctaacttac tttctggtat
     3061 tttagttatg ggaatgctat actctatcaa tttaagaata atgggaaaat caaatattcc
     3121 tctatttagc tttaaacact tattcaatgg ggaaatatct cctatagttt tagccttagc
     3181 ttttgtattc atctgtaaag tccttttaga tttattccta aaaacaggtc ttggatacac
     3241 tttaaaaggt gttggtgata actctcaaat gattaaatcc ctaggtataa atatcggttc
     3301 tataaaaata ctaggactt
//
LOCUS       DBGJFU010000229         3312 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.233, whole genome shotgun sequence.
ACCESSION   DBGJFU010000229 DBGJFU010000000
VERSION     DBGJFU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.233"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..781)
                     /gene="dccA"
                     /locus_tag="ACOIAS_003711"
     CDS             complement(<1..781)
                     /gene="dccA"
                     /locus_tag="ACOIAS_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP diguanylate cyclase DccA"
                     /protein_id="HGZ2703375.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVE"
     gene            complement(812..3046)
                     /locus_tag="ACOIAS_003712"
     CDS             complement(812..3046)
                     /locus_tag="ACOIAS_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGZ2703376.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         1159 a          462 c          370 g         1321 t
ORIGIN      
        1 attcaactat tgtcaatttt aaatttcttt ttactgtatc aacaccataa ctaaatgaaa
       61 gatacttata cttagagaat acttttaaaa aattttctct taaggcttct agttgtgaat
      121 aaacataatt tttgtcaaca tcactagaga atataacaat aaattcatct cctcctaatc
      181 ttataataga agtagaattt attaattctt tatttaaaag tgaaacaaaa tcacataaaa
      241 tcttgtctcc tacattatga ccttcatagt catttatcat tttgaaatta tctaagtcaa
      301 ttagtacaat ataatctcct ataaatatgt caaatctatc atttaaatat tttctattat
      361 atgctccagt taatgggtca gtaattgagt agtgaagatt tctcttattt tcttcataga
      421 gttttgttat atcattggcc atagtaatta tgtaggtttt ttcatcaaca tctactttat
      481 attctaaaac attccaatag cttgtagtat cttttttaaa ctctaaaatc caatttgaaa
      541 ttttatcata aggataattt ttttcccaat ctaattgaat attgtgagaa tccgtaaaaa
      601 atttagcaat attatcaggg agtttcttag ctatttctat attagtcatt ccaagtggat
      661 tatcttgcat ataactttta attaaatttt ttaaattatc atttataaat ataattctgt
      721 agttagaatc tcttataatt agaaatcctg gaaaagttct taaaaaaatt tctttaaaca
      781 tattaatttc tccaaaataa aatactttgt actagatttt tgttaaattt tttattttat
      841 ttacatctac atacataccc tgggcaatcg accttccatc aattgcagaa aggttacatt
      901 gtgtatacat aactgtgaca atttgaccat ctcgttttat gaatctatat aaaaatggtt
      961 caattttatt tgtgtcaatc atatccaaac tttttttata aacaaagtct aaatcatctg
     1021 gatgaacaat tgctcttaaa tttccgccca tctcaccaaa aaactgttct tttgtataac
     1081 ctataatatc aaacatttct tcattcatat aagtaataat catatcacca aataaatcgt
     1141 agcgaattat acctacaggc aacattgaat aaaattgtga taaaatatta ctttcatcaa
     1201 gatgtgtatt tgtcatttta ggtattgaat atgattcaaa ttcatttata tagccagcag
     1261 ttttaattaa atccaaatgt aattttgcta agagtaattt attttctgaa attatacaat
     1321 ttgcactaaa gtagaaattg ctatattttt gatgttcaga agcttcatct gataaaacgg
     1381 cttctccact aattaataat gaatctgatt cagtattatg tggggttata cttttgtaaa
     1441 gaatagaaaa ttttttacct ttaatatgtt tttcaaaatg ctctattact ttaggaaaag
     1501 atgtaatttc agttttgttg taaaaatcaa accattgaat gtcttcagtg acattagctc
     1561 ttagtttttt catatcttgc ataataaaaa atccatcaaa tatcttctct attaaagatt
     1621 ttcctatatc agaatatttt ataaacattt tctctgaatt attctcttta gatagtaatt
     1681 tttcaaattc tttgcactcc atgggttttg cataataata accttgggcg atatcacaat
     1741 ttatactttt aataaattct acatgttgtt ctgtttcaac accttcacat actgtaagca
     1801 tcccaagagc tcttgcaatg tttattatag agtacaaaat ggttgtattt ttaaaatcat
     1861 ttaaattatg agataaaaat atcctatcaa ttttgattat atcaaaaggt agattttgca
     1921 acataccaaa agatgaatat ccagaaccaa aatcgtccat tgatacagaa aaaccataag
     1981 aacgaagttc agctagttgt tgaataattg tatttgtatt ttctattaat atattttctg
     2041 ttatttcaat gctaaaattt gatgtagaga cgctgtactt ttcagtaata ccttttagca
     2101 tacttggaaa attagggtta aaaaaatgaa gacgagaaaa gttacaacaa attggaacaa
     2161 aaacatcatt gttatcaatc catcttctta tatttttaca gttctcttca aataaatgca
     2221 aatctaattc cgttatcaga ccaaactgtt caaataaagg aataaattta tctggagtaa
     2281 gaagtccttt aatagggtgt ctccatcttg caagtgcctc acatccaact atagtattat
     2341 tttttatatt ataaagtggt tggtaaaata cttcaatttg tttagtttgt agaacatgtg
     2401 atatttctga taagaactcc ttatcatttt ctaatttatt gtaaaaacta ttatctttaa
     2461 aatcaaatag aatgtatcta tttttacctt cacttttagc cttttttaaa gcactatata
     2521 cttggttgaa aagcatcaaa gtagtcatgt ttggttcacc attccagata gtgctgccga
     2581 tactaaatgt aacattggag tatatttctt tttcattagg aagttttatt tttaattctc
     2641 gggcgctctc tataatagtt gcacccatat tatttaactc tttatttgat acatttggaa
     2701 gtacaattaa aaaaccttct ccttttaaat aagcgactat tccctttgat tctggaataa
     2761 agtgcaatag gttttgagtt agagtaatta ataaattttc tccgactagg tatccatata
     2821 tatcattaaa taatttaaag ttatcgatat ttaataacat gattcctata tgtatatttt
     2881 tcgaaataca gtcatcaata tgttggtaca taaattctct gtttggtatt ttagaaaaaa
     2941 aatctatttc atttaaggtt tttaatttaa aatttctact atctaattcc ttagataata
     3001 cttctacctt tttatttaac atttttatat aattatgatt ttgcatggta ttcctccata
     3061 agatacttta aattttaatg acaactgtat ttatgctaga agtatttaaa tgttaatttg
     3121 taaataattt aaatgctaaa ttttaaataa gtatttaaaa tgaatataca ttaagttact
     3181 tgctccaatg ctacaaatac aaatattttt tagtttagta ataatattag ataaaattag
     3241 ttaatgctta aattctagta tattgaaaga tttccttttt gaatgcaatt atatcatatt
     3301 ttttcataaa ta
//
LOCUS       DBGJFU010000230         3079 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.235, whole genome shotgun sequence.
ACCESSION   DBGJFU010000230 DBGJFU010000000
VERSION     DBGJFU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.235"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            25..819
                     /locus_tag="ACOIAS_003713"
     CDS             25..819
                     /locus_tag="ACOIAS_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703377.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            820..2880
                     /locus_tag="ACOIAS_003714"
     CDS             820..2880
                     /locus_tag="ACOIAS_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HGZ2703378.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1172 a          314 c          504 g         1089 t
ORIGIN      
        1 aacgagaaag gagtggtttg ataaatgatt gaggtagata aattgtgtaa gtcttttaca
       61 agggttgtaa aagatgataa gaataaaagt tcaataagaa agctaaagaa agtaaaaact
      121 aaaaaagaag aattttttgc tgtaaataat gtttcattta aagttatgga aggtgaaatt
      181 gttggaatat taggtccaaa tggtgcagga aagactacat tacttagaat gttaggtggc
      241 atattaacac caacatcagg aagtataaat atttctggat atgattattc aatagataga
      301 aattcagcaa aaaaagaaat aggttatctc tctggaaata caaaactata tggaaggctt
      361 tctccgagag aacttctaac aacttttgca agtctatatg agatgtcaaa agaagatata
      421 gaagaatcaa ttgaaaatat tgtaaaaata atggatatga gtgaatttat agataataga
      481 attgaaaatc tatcaactgg tcagactcaa agaacatcta tagctcgttg tttaatacat
      541 tctccaaaag tatatatttt tgatgagcca acactaggtc tggatgtttt aagtagtgca
      601 tctattatag attttatgaa gaatgaaaaa ctaaggggaa aaactgttct ttactcaact
      661 cattatatgg aagaagcaga aactttatgt gacaagattt ttatgattca caatgggaaa
      721 ataattgcat caggaacacc acagagttta aaagaggaat ctggagtaaa taatttgaga
      781 gatgtattca taaaacttgc cagaaaggag gacatttaaa tgagaagtaa gattgtaaaa
      841 tacatattta aaaaagaaat gttagacata ttgagagata aaaagacttt atttatgatg
      901 attgttgtgc cacttctttt atatccttta cttatgttat taatgattca agtaatgaat
      961 atgtctgcaa gtagtatgaa tagtaaggac attaatatag cttttaataa taaacctaat
     1021 aaggttttag tatccatgat tgaagatgat aatttagaaa aagatactaa aaatacttca
     1081 aatgataaag gcaaaataag agtgttagat gtagataact ataaaaagtc attagaagaa
     1141 aataaaattg atgcttatat aaagatggaa gaaaaaaaat catctataaa ttatcaaata
     1201 tatataaatt cttctcaaga aaattcttct aatgcactaa aaagaattga aactgttttg
     1261 gataaatata aaagatttac catagaaaaa actttatctg aaaaaggatt agatgtacaa
     1321 gctactttag aaccaataac atatagcact gtagatgttg caaagacgga agaagtggca
     1381 ggatactttt taggtcagat acttccattt atattaataa taggagtttt actaggttca
     1441 atatatccag caatagatgt tatggctgga gaaaaggaaa gaggtacatt agaaacatta
     1501 tttacactgc caatatcaaa cttagaactg gttatgggaa aatatatggc agtttctctt
     1561 agtgcaatag taacagcaat tttaaacatt ttatctatgg gactgactat ggcatttatt
     1621 ttagttagtg gaggtattag tagccaattt ggctttggaa actttaatta tggagactta
     1681 gtcttaccaa ttataactac tttaatttgc atatgtcttt tttcaatggt agtttcagca
     1741 ataagtatgt gtgtatgttc attagcaaaa agttttaaag atgctcaaaa ctacataaca
     1801 cctcttatgt taattattat gataccatca tatgtttcta tgataccaaa tatagtatta
     1861 gatagagtga cagctgtaat acctgtagta aatatatctt tacttataaa aagtgtatta
     1921 tcttttaaaa gcgatttaaa tttaatagcc ttagttttag catcaaatgt agcctttgtt
     1981 atattggcaa tcatactact gtctaagatg ttcaattctg aggaaatact ttttggtaat
     2041 agtaagagtt tctctttctt agagaaaaga agtaatataa aaaaagggtc tataccaact
     2101 gtaagtgatg gtgttatttt atatgcagta ggattagttc tattgattta tgttggttct
     2161 ttagttcaaa ttaaatttgg tatggcaggt attataatga cacaattcat gataatttct
     2221 ttaccgttat tatttgcata ctatattaaa gctgatttta aagaggtatt tagcttgaaa
     2281 gttcccaaat taaagcatgt gtttgggagc attgttcttt ggattggtgg atacatactt
     2341 attatgatta taacacagct aattttattc ttatttcctc aaaatatgga agttgctgaa
     2401 agtttaaata aggctctttt tatcaaagat agtgttttac tgaatttact agcaatagct
     2461 ttattaccag ctttatgtga agaaatgttt tttaggggat ttatattttc ttcatttagt
     2521 aaatcaaaag ataaaaataa gtctatcaag ctagctataa tatgcagtgg ggtactattt
     2581 ggtattatgc atatggattt tattagaata attccaacat caatattagg tattatattt
     2641 gcatatagtg tgtataaatc tggttcaata tttgtatcaa tgttattgca ttttttaaat
     2701 aatagtgtag cagttgtgtt aaatcattat acaactggaa atataaccaa cttatataaa
     2761 tttgttgaag ttgatttttc gaacttaaat gtacttaaat ttgccttaat tcttctaata
     2821 agtcttatat taattatgtt aggattaaga gttttagata gaaagtcttt aagaaattaa
     2881 gtataaagtt attatataga ttaaaatatt aataagcact aaagtatatg gatatatctt
     2941 gtatataaaa atactttagt gctattttat atattttaat tatatgaatt atttatcgtt
     3001 tcatttatgt aatgaaaatg ttatataaat gaaacaataa ataattttta ttttagaaat
     3061 ttatatttaa ataatcaag
//
LOCUS       DBGJFU010000231         3064 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.236, whole genome shotgun sequence.
ACCESSION   DBGJFU010000231 DBGJFU010000000
VERSION     DBGJFU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.236"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            133..1401
                     /gene="eam"
                     /locus_tag="ACOIAS_003715"
     CDS             133..1401
                     /gene="eam"
                     /locus_tag="ACOIAS_003715"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HGZ2703379.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1494..1850)
                     /locus_tag="ACOIAS_003716"
     CDS             complement(1494..1850)
                     /locus_tag="ACOIAS_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703380.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2001..>3064)
                     /locus_tag="ACOIAS_003717"
     CDS             complement(2001..>3064)
                     /locus_tag="ACOIAS_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGZ2703381.1"
                     /translation="LTVVVSYILIKVIDNYKYFFGVISLLMSLLTPFIIAFVLAYIFN
                     PLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADLVAQIPTYIDKT
                     EQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFSTTFSVGKFIVQF
                     LLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLNIGKYFTGKILD
                     SFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVIINLFSNPTIAL
                     TSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIPGMILSAPVMGV
                     IKIYFCEFVNRRHDKIQME"
BASE COUNT         1241 a          415 c          449 g          959 t
ORIGIN      
        1 aaattaattc aatttcattt gttataaaat ctatatggca aaattaaatc tgcctatcag
       61 aatgatataa acacatcaga aaggaggacg ctatcaaata atacaagtag ttaactttga
      121 tagcaaaaaa ttatgaatga acaaactaga atatccttag agagagctgc tgaattaaaa
      181 tcaaaaattg atgattatat tcaggctaga aaaacgatta acagaggtct tgaaaaagaa
      241 gaagagataa ataaacgaaa acagaaaata ttaagtatct taaatggaac tgaagaggat
      301 tggaataact acaaatggca attatcaaat agaataacag atgtagatac tttatcaaaa
      361 attataactc taactaaaaa agaaaaagaa tatataaaag aggttggtac tcaatttaga
      421 tgggcaatat ctccatatta tttgagtctt atagacccag aagatatatg tgacccaata
      481 aaattactgt ctataccaac acatatagag ttggaagatg agcaagaaga tttggaccca
      541 atgggagaag agtatacaaa cccagcagga tgtataacta gaagataccc ggatagatta
      601 ataataaatg taacaaatga gtgtgctatg tattgtagac actgtcagag aagaagaaat
      661 attggacaac aagattctca taagtcaaaa gctattatcc aagaatctat agactatatc
      721 agagaaaatg aagaaataag agatgtacta gtaactggtg gagatgctct tacattaaaa
      781 gatgattatt tagagtggat tcttagccaa cttaaagaga taccacatgt tgattatgtt
      841 agattaggta ctaggactct tgttacaatg ccacaaagaa ttacagatga attttgcaat
      901 atgctaaaaa aatatcaccc tgtatatata aatactcatt ttaatcatcc aatggaaata
      961 actaaggaat ctaaagaagc ttgtgaaaag ttagcaaatg caggcgttcc attaggaaat
     1021 caggcagtat tattaaatgg aataaataat gataaatttg taatgagatg tttaaatcaa
     1081 gaattactga aaataagagt aaaaccttat tatatattcc aaagtaaaca tgtaaaggga
     1141 acaaaacatt tcaatacatc agtagatgat ggtcttgaaa tcatggagta tttaagagga
     1201 tatacatcag gaatggctat accaacatat atagtaaatg ctccaaaagg aggaggaaag
     1261 actcctttgc ttcctcaata ccttgtatca aaaggaacag attacgttat gcttagaaca
     1321 tgggaaggaa aagttataaa aatggaagat gaacctgctg tagatataaa gaaacttata
     1381 aaagaacaag cacaggatta aaaaaatatt taaagtttat aaaaagaaac aactttaaaa
     1441 taaaaagaaa atggatgtca aatttatatc aatttgcatc cattttttat tagttattta
     1501 ttattttgtt gttgattaac taattcaaga tatccacttg ggtggaagta atatatctca
     1561 gaagtatttt catttacaaa gtaaccaata tcaccatctg catctggtaa aaatacataa
     1621 ccattaagat ttttagcttt taatgcttca aaatcactag catttccttg atatatgtaa
     1681 tcaacttcat tgttgtattt tactaaaagt tctttagctt gttcttgagt caatttagta
     1741 ttttggctta caggaacatt cttttgagaa tctgcataag aagattcatt tactggtgca
     1801 gttaataaca aacaagataa aactaataaa gatataagtg cttttttcat actaaaaccc
     1861 tctttcatgt ttaattatat aagattcatt acttaataat attgtatgtt tcctataatt
     1921 aaatattacc attaaagaga ataatattac agttacacag ctgtaacttt tgattacaaa
     1981 atcgtaatta aattgtattt ttattccatt tgtattttat catgtctacg atttacaaac
     2041 tcacagaagt atatctttat aacgcccatc acaggagcag atagaatcat accaggtata
     2101 ccaaagaatc ctccaccaac agtaactgct aggattgtaa agaatgggct aagacctagt
     2161 tgtccaccta cgatttttgg ctctataaat gtaacttcaa cttgttgaat tattattaag
     2221 taaattaaag aagttaaagc aattgttgga ttggagaata aattaattat tacaactggt
     2281 gccataccta ttactggacc aaaatatgga accatattca tgacacccat aagtgtacca
     2341 aataatagtg catattcaga ttttaaaaag taaagaccaa ttccagaaag tactccaact
     2401 ataaaagaat ctaaaatctt acctgtaaag tatttaccaa tatttaaatt taagctttga
     2461 catagttcta ttataaagtc tccatatttt ttacctaagg aaatataaac tacttttttt
     2521 gtaaacagta agaatttttc tttttctaat aatatataga aacaaattat aaaacctaaa
     2581 agaaattgta ctataaattt tccaactgaa aatgtagtgc taaatatccc accaagtgaa
     2641 ccaataagca agttactaaa tttaggcatt acactcataa ttttgtcacc atactctttt
     2701 aatgtattag ggtcaactga ttgtaaactc ttgcctaagt cgaataaaaa ctgctctgtt
     2761 ttatctatgt atgtaggaat ttgagcaact aaatcagcta aactatttac tatggatgga
     2821 actgtaaata atataaacga aattagtata actatgagta caccataagt aagaagtaac
     2881 gaatatattc tcttaaaatt taatttactt tctaagaact ttactagtgg gttgaatata
     2941 tatgctaata caaatgctat tataaatggt gttagtagtg acattaataa acttatcact
     3001 ccaaaaaaat atttgtaatt atcaataact ttaatcaaga tatatgaaac aactacagtg
     3061 agta
//
LOCUS       DBGJFU010000232         3042 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.237, whole genome shotgun sequence.
ACCESSION   DBGJFU010000232 DBGJFU010000000
VERSION     DBGJFU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.237"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            87..2444
                     /locus_tag="ACOIAS_003718"
     CDS             87..2444
                     /locus_tag="ACOIAS_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HGZ2703382.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2530..2847)
                     /locus_tag="ACOIAS_003719"
     CDS             complement(2530..2847)
                     /locus_tag="ACOIAS_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGZ2703383.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1170 a          330 c          495 g         1047 t
ORIGIN      
        1 agaataataa aataaaaaaa ttgagaattt tataatatat ttgtagattt gagtataaaa
       61 aagtgctaag ttgaaaggag taaaatatgg ataaggttaa tttagaaagg tatagttgtg
      121 atataaatgt tggtttaaat aaaaatcaac ttcaaagtag aatagatgat aatttaatta
      181 atgcatttga taatggaaaa accaaaacat ataaacaaat ttttaaagac aatatattta
      241 cactttttaa tctcataaat attgttttag tctgtttatt agtttcagtt ggttctttta
      301 aaaatacctt atttatattt attgctgtaa ttaatactat tataggagta atacaagaga
      361 taagagctaa aagattattg gacaatctat ctgttatagt ttcaaataag gtatctgtca
      421 taagagaaaa agagaaaaaa gagattgatg tacaagaatt agtattagat gatattatga
      481 ttttaaaaac aggtaatcaa atttgtgcag attcaaaagt tttaaatgga aaattagagg
      541 taaatgagtc tttggttaca ggtgaatctg atattattat aaaaaataaa ggcgattttt
      601 tatattctgg aagttttgta gtttctggag aagcttttgt aagagttgaa aatataggaa
      661 aagataacta tgcaaataag atagctaatg atgcaaagat ttctaaaaaa cataattctc
      721 aacttagaaa ttcattaaat gcaatattaa aaattgtagg tataataata attcctttag
      781 gaattatatt gtttgcaaaa caacattttg gaaatggaga ttctatagct acatctgtag
      841 taggtacagt ggcagctgta aatggtatga taccagaggg attaacatta cttacgagca
      901 ttgctctagc tgtaggaact ataaaacttg ctagtcataa aactttagtg caagaactgt
      961 actgtgtaga gactcttgct agagttgata cactatgtct tgataagaca ggtacaatta
     1021 cagaaggtaa aatgcaagtt gatgatatag taatgttgga agaagtggat attaatgaaa
     1081 taatggggaa tatatgtaat tctctcaaag atgataatgc aacactaaat gcaataagag
     1141 ataagtataa tgttttagat acgtataaag ctaagaattt aataccattt tcttcagaaa
     1201 gaaaatacag tggggttacc tttgaaaata aaggtacata tttattaggt gcatttgagt
     1261 ttatatttaa ggaaaaaaat aaaaaactta ggatagaagt tgaaagatat tctaaaaaag
     1321 gaaacagagt aatagtttta gctcatagtg attcttatat ggaagataat aatattcctg
     1381 aagatataaa gccactagct tttatattga tattggataa aattagagat gaagctaagg
     1441 aaactttaga atttttttac aatgaaggtg tagatataaa aattatatct ggtgacaatc
     1501 ctataacagt aagtgaggta gctagaaaag caggtcttaa aacagctagt aattttattg
     1561 atgcaacaac acttaaaaat gatagtgata tatacaaagc agttgataaa tacagtgttt
     1621 ttggaagggt aagcccacaa cagaaaaaac aaatgatttt agcattaaaa aaacaaaatc
     1681 atacagtagc tatgactgga gatggtgtaa atgatgtact agcacttaaa gaagcggatt
     1741 gtagcattgc catggcatct ggaagtgatg ttactaagaa tgtgtctaat ttagtcttat
     1801 tagattcaaa ctttgcatca atgccaaagg ttgttatgga gggaagaaaa gtaattaata
     1861 acattcaaag agcatcttct ttgtttttag taaagacaat tttttcattc ttcttgtcgt
     1921 tattaacatt attcttcttc agtagacagt atccatttgt tcctatacaa ttatcactca
     1981 ttggggctgt aacagtgggt ataccatcat tcttcttagc tcttgaagca aacaaaagta
     2041 gaatttcagg aaatttttta ctcaatatat taaaaaatgc tttaccagga gcactatgtg
     2101 tgacagtaaa tgtaattatt gtttacaata tagttgaata tttaggttat agttcaagtg
     2161 tattttccac aatgtgtgct atattaactg gtgtatgtgg tcttttaata ttgagacaac
     2221 aatgtttgcc atttaataaa gaaagattat ttttaatagt aagtatgact attgcatttg
     2281 ttattgggat tctattttta ggtggattat tctcatttgt agcattgagt agagaattaa
     2341 ttctcataac tatggcatta gcaatattaa tgccaattat gataaaagtt atgttatttg
     2401 tcttagatga gatattagag acaattgttc cagatttaaa ttaattttaa aatgatataa
     2461 ataagagcag ttttagttga actgctcttt ttagatgtgt ttaattttat gtaagaatat
     2521 gtatttttat taatcagcta tatatgagca acagtagtca acaacttcat taacctttaa
     2581 gtcaaaactt gaatcactag gaacattaaa tttttgccct tctttataag ttacaaactc
     2641 attgcttcca ggcagtttta catccatact tccagcaagc atctccatta tttcttcttc
     2701 tctagtagaa aaagtgtatt ctcctggcaa cattaatcct aaagtttttc tttctccatt
     2761 aggcagtatt attactctgc tacttacctt accatcaaaa taaacgtttg ccttctttac
     2821 tgcagtaaca ttcttaaatt cactcattag ataaaacctc caaaattatt tgtacatata
     2881 tattatataa aaataatgta aaaaatgcta tatatttaca aaaaaattta gtatttatta
     2941 aaaaaattaa taagaataaa aaagaggtga aattgcttaa attgataaaa aatataaaat
     3001 aaattgcaaa gtaagcaaga aaagaataag ttgaagagtt at
//
LOCUS       DBGJFU010000233         3040 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.238, whole genome shotgun sequence.
ACCESSION   DBGJFU010000233 DBGJFU010000000
VERSION     DBGJFU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.238"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            65..1192
                     /locus_tag="ACOIAS_003720"
     CDS             65..1192
                     /locus_tag="ACOIAS_003720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGZ2703384.1"
                     /translation="MKGKITMDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEG
                     KDVIAEAQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESK
                     DVSILAAYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQ
                     MLLMGFKNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLN
                     NIKQEVVETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLH
                     SDIAQSKRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIG
                     RTGRAGEEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1569..2753)
                     /locus_tag="ACOIAS_003721"
     CDS             complement(1569..2753)
                     /locus_tag="ACOIAS_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703385.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1152 a          448 c          407 g         1033 t
ORIGIN      
        1 aaatttacta aaaatactta taaatatgat attattaatt ggttaataaa aaagaaaatt
       61 tttcatgaaa ggtaagataa caatggattt caaaagctta gggattagtg aaaatacaat
      121 aaatattcta aaaaaatcag gaataacaac acctacacct atacaaaaag aaagtattaa
      181 actaataaaa gaaggaaaag atgtaatagc agaagctcaa actggaacag gaaaaacact
      241 agctttttta cttccgatat ttgaaaatat atcattagat ataaatgata tacaagtact
      301 tatactttct cctacaagag aactagctat tcaaataaca gaagaagcta tgaaacttaa
      361 agaatctaaa gatgtaagta ttcttgcagc atatggagga aaagatattg gttctcaaat
      421 aaagaaacta aaaggaaata ttcatatgat tatagctaca ccaggtaggc ttttagacca
      481 tctaaataga aaaacaatag accttagtaa gttaaagact tttgttttag atgaagcaga
      541 ccaaatgttg cttatgggat ttaaaaatga agtggaagct attttaaaag agacttcaaa
      601 caaaaagcaa acactatgct tttctgcaac tataaattct caagtaaaaa aattagctta
      661 tagatacaca aaaaatcctg ttgtagtttc tattcaaaaa gaagaaatca ctcttaataa
      721 tataaaacaa gaagtagtag aaactacaga tagaaaaaaa ttagatgcct tatgtaaggt
      781 actagatgaa gataatcctt ttatggcaat aatattttgt agaactaaga gaagggttga
      841 caatcttgaa gaagctcttg ctataagagg atataattgt caaaaacttc atagtgatat
      901 tgctcaatca aaacgagaaa gaataatgaa atcatttaga aatttagata tacaatactt
      961 aatagctaca gatgtagctt ctcgtggtct tgatatatct ggagtaagtc atatatataa
     1021 ttacgattta ccagaaacac cagaagacta catccaccgt attggtagaa ctggtagagc
     1081 tggtgaagaa ggctatacat gtgcttttat tgaccctaaa aatgaacgaa tgttaagtga
     1141 aatagagacg gctatagaat ctaaaattag tagaagaatt atagaattat aatttataga
     1201 ttgcttaaaa taccaatgcc acacaaacac tgtaatatat agaaagctac agaaaacatg
     1261 tatcaatcta tactttgctg ttatactatt ttatattaaa gaaaaaagag gctatccaaa
     1321 gtaggattag tctcttttat tgtcaaaata atgaaaatat atttgacttc atataaaaaa
     1381 gagtgcctta taaactaaaa gtccattttg agacaccctc tttttatctt ttattcttag
     1441 ttttatattg cttgtttata gttgtttaat attagatttt gcaaataatc ttaataaaca
     1501 tgcagatata ttatctatag tattcttatc tatagtattc tctttagcaa tattttttgc
     1561 tactacatct aatttacatt ttcatttaat tccgctggcc atttagcttc tggattttcc
     1621 tttgcaaact tcttattctt ataatgtcct agtgtaagga atggaattac tatagtgaat
     1681 agaccaagat aaccacaata tccatatcca tatttaacaa tattacttaa tcctgtaagt
     1741 gatataagca ttgacactac cattattgca caagctgtaa atacccttct tttcattctt
     1801 gaagaaagga aagaaagtgc tttatggtct tcaaatctat ttacaagacc aaatatagta
     1861 gttactcctg tagacattaa acaaaggaat aatgcaacat tataaattat gtatatagta
     1921 tgatttccag attgacctgc aacataaaga gtagggagtg ttgtttgccc agcagcaata
     1981 atttctctat accatcccat tagcatagtt acagcaagtc ctaatgcaat tgcattcata
     2041 acaaatgaaa ttatcattgc cttagtagct tcttttgagt tcttaagaag tggtcttgaa
     2101 catcctgcaa gtgtaggtat aacaaccgat tgaaaacctg cataaataaa agtattaagt
     2161 attggcttga tatagccagg tgtgaaactc gttctatttg ataatatttc agttatttct
     2221 ggtactttag cttttatacc aacaataaaa atagtaagag tacatactag aatagctatt
     2281 gtcattgctg tagctgcttt agatacaagc atagcaccaa atatagtaaa gacaagaagt
     2341 acaactccaa taataaatac agctacaaaa taatttactc ctatactttg aaatactgct
     2401 gctgcacctg ctataacagc acttacagct attaaaacca ttagatagta atatatctcc
     2461 catattattt caagttttgg atatggtctc caaagctcac aaaataaatc cttataattc
     2521 ctacaattgt tattattata cattatgata acttctctca tggtagcagt aagtataatc
     2581 attgctaaaa ttgccataag tggtgctgtc catccatact gcacatagta ctgagtttct
     2641 tgattccctg tagcaaatcc tcctcctgca tgtgaactaa aaagaacgct tgcaacacca
     2701 aaaatggcta acatagagcc cgttactttt gctttcttta cacttttttg catgaaatca
     2761 tcctccctaa aatgatatta aaagttttta ttttaacact ttattgataa aacaataaca
     2821 tttttgctca ttaaattcaa ctatattttt ccaatgactt tacattttca acttatttct
     2881 acctttatat taaatatttt ttatatattt attttatttt ttaaatagta ttcaaattca
     2941 ctttgttttt taattttatg aaatatcttt tttaatgtta aatattatac attttttctt
     3001 tattattata taattttttc tttattattt tataataata
//
LOCUS       DBGJFU010000234         2929 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.239, whole genome shotgun sequence.
ACCESSION   DBGJFU010000234 DBGJFU010000000
VERSION     DBGJFU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.239"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            111..1382
                     /locus_tag="ACOIAS_003722"
     CDS             111..1382
                     /locus_tag="ACOIAS_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2703386.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     gene            complement(2022..2777)
                     /locus_tag="ACOIAS_003723"
     CDS             complement(2022..2777)
                     /locus_tag="ACOIAS_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HGZ2703387.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT         1079 a          399 c          346 g         1105 t
ORIGIN      
        1 aatagtattt atagtttaga tttgatataa tacatgtggt aaaattttga tttgtaaaac
       61 aaaattttac ctacatatat taaaacaaat ttaaaactaa ggagacctat atgttaaaag
      121 tattaaaagg aaatataatt tttacaaaaa cttcagaaac tttcacagta tttgaaaata
      181 gttatattat actggaaaaa ggtaaagtaa agggaatatt taaagagata cctaaggaat
      241 acaaaaacct aaaagtagta gattacactg ataagcttat aatacctggt atgaatgacc
      301 tccatgccca tgcctctcaa tttaaaaatc ttggaatggc tatggataaa gagcttttgc
      361 catggcttga aacttataca tttccagaag aatcaaatta taaagatata gaatatgcaa
      421 caaaaatgta taaaaaattt gtaaaagaac tttggaaatc tggaactaca agaatagcag
      481 tgtttgcaac agtacataaa gaagcctcta tgaaacttat ggatatattc aaagaagttg
      541 gtcttggagc atacgttggt aaagtaaata tgaatatgaa ttgccctgat actttattag
      601 aaaacactca ggattctatt actgatacag aagaaattat aaataagtat agtgatacag
      661 attctattgt aaaaccaata ataacaccta gatttatacc aagctgtaca agtgaacttt
      721 taaaaggttt aggagattta tgtttaaaat ataatattcc tattcaatca cacttatctg
      781 aaaattatga tgagattgaa tgggttagaa gtctagaacc tgaatctaaa ttctatggag
      841 atgcatataa tagatataat ttatttggtc aaactcctac tttaatggct cattgtgttc
      901 attgttctca ggatgaaata gatttaatga gagaaaataa tgttatggca gttcactgtc
      961 cagcttctaa ctttaatgtt ggtagtggtg ctatgcctat cagaaaatta atcgataata
     1021 atattagatt agcacttgga tcagatataa gcggtggaca tacattgtca atatttaaag
     1081 caatggtatc tgcaatacaa ttatctaaat tatattgggt taattctggt aagaagtata
     1141 atttcttatc tttatctgag gctttttata ttgcaaccaa aagtggtgga agtttctttg
     1201 gaaaagttgg tagctttgaa gaaggttatg attttgatgc attgattatt gatgattcaa
     1261 atttaaatca tgacaattat tctatacttg aaagacttga aagatttatc tatgttggtg
     1321 atgatagaaa tatcattcat agatatgtat gtggaaaact aattgaagaa cctaatatat
     1381 aattataata aaaattttaa ttatatatta tggttaattt atacaactat aattaataaa
     1441 agagtatatt ttaaatctaa aagctatagg ttgaaacata ctctttttat tttgtttatt
     1501 tatagcttta gttattaatc atatataaat ctacaaaacg tattcctagt aacttatatt
     1561 ttttgatatc tacaatcata agactatatt attggattca tacctcaaaa atattgcctt
     1621 ctgaacatta ttatattcaa agctatgtta atttacaatg atatatccta acatgtattg
     1681 attaaaaaaa attaaaactt gcttttcatc tcctatatct aaagaagtgg tatttagaat
     1741 aaactattgt aatattaaag ttgaaatttt ctagtttatt tactctatag caccatattt
     1801 taatactatt aagtaaaaat gtagttaaaa tatatactaa tatccctgaa ataagtattt
     1861 cttttttatt tagcacactt attcgtaagg tattagttat tatatttcca aattatagaa
     1921 taaatattat acttattgct aattataata ttaatactgt taagatattt ttcttcatag
     1981 ttatacccat tatttaatac aatttattat aaattcaatt tttaaacttt tcttatctcc
     2041 ttatcagaat aaaaatcccc tttacttcct tttattttta ctatttttaa acttgccatc
     2101 ttctctaatc tccaaagtat aaaccaatct tttacacgca tttcatcttc aaacatacag
     2161 tttgcaacca cttcaattaa aggttgccat ttgtcactga tatttcttaa tatatattta
     2221 tcaaaataag tttcttcaac acttttaatt tcgttaccta ctaatgctct aaaaatagaa
     2281 ttttctttaa ctaacacatc ccatttattt ttaaattcta gtttttcatt aaagccaatt
     2341 tctagtgtct tatctaaaaa gaatggaaaa tcttctggag acatttcact ggtaaatgaa
     2401 tatctaatta gagcatcttc tgtctttttt tcatctacta atgcaatctt tatattttta
     2461 tcttgaaact tactcactac ataatatact ccacacaaat cttttgggtt atttgcacac
     2521 catattatta tatttctaca ttttgataat ttcctatctt ctaacaaatc tggcttgaat
     2581 aatagtttcg gattattttc aaaaacattt ctaatcttaa aaatattttc tcttaattcg
     2641 tagttattta catcaaaaat attacctatt gataaatcat cttcaaataa tacaatttta
     2701 ttatgtggta ttaactttaa cttcattgct tcttttaata acaaataaca aatatcctca
     2761 aagcatatat gtgccataaa attcacctct tttaaaatta acaatttatt ttattataat
     2821 atataaactg taattctgaa cctcccatga ctaaagtcac agggttcctg cttcacagaa
     2881 ttttgcatac taaaatatgt cttactaatt ctccacaggc tatccccgt
//
LOCUS       DBGJFU010000235         2826 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.240, whole genome shotgun sequence.
ACCESSION   DBGJFU010000235 DBGJFU010000000
VERSION     DBGJFU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.240"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..478
                     /gene="bilQ"
                     /locus_tag="ACOIAS_003724"
     CDS             <1..478
                     /gene="bilQ"
                     /locus_tag="ACOIAS_003724"
                     /inference="COORDINATES: protein motif:HMM:NF045593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bilirubin utilization transcriptional regulator
                     BilQ"
                     /protein_id="HGZ2703388.1"
                     /translation="HIIIIEGKFKMDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYK
                     LTNGLYFYLIYINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRA
                     FQLRVTDKGEEVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            480..1574
                     /gene="bilR"
                     /locus_tag="ACOIAS_003725"
     CDS             480..1574
                     /gene="bilR"
                     /locus_tag="ACOIAS_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin reductase"
                     /protein_id="HGZ2703389.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADRNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1662..2192
                     /gene="bilS"
                     /locus_tag="ACOIAS_003726"
     CDS             1662..2192
                     /gene="bilS"
                     /locus_tag="ACOIAS_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HGZ2703390.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2543..>2826
                     /locus_tag="ACOIAS_003727"
     CDS             2543..>2826
                     /locus_tag="ACOIAS_003727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2703391.1"
                     /translation="MPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWL
                     QIHAFYSDTIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILV"
BASE COUNT         1151 a          279 c          482 g          914 t
ORIGIN      
        1 acatattata attatagaag ggaaatttaa gatggatttt aaaaatttac agtatgaaag
       61 tttgtcatat attatttgta acttgcaaaa aaactttaaa ctttattgtg aaaaatgttt
      121 aaaaccatat aaacttacga atggtctata tttctattta atttatatta ataaaaatag
      181 gaattgttct ttgaatgatg tttctacaga atttgaagtt gataaagctc atacaacaag
      241 aactatatct aggttagaac aagatggata catagaaaaa atccaaaacc ctaatgatag
      301 tagagctttt cagttgagag taacagataa gggtgaggaa gtattagggg atataaaaaa
      361 catattttca aagtgggata atcacataaa aaaagagttc tcggatacag aatataagga
      421 actggtaaag aatttacatg tggtaaagga tataaaaaca gctgtggagg aagaatagga
      481 tgtattgtaa tttattaaaa ccaattaaaa ttggaaattt ggaattaaaa aatagagtat
      541 cttttgcacc tacaagcatg ggtcttaaat tggaagaaaa aattaaaaag ttttctgata
      601 ttgctaagtc aggagtagct ctgattactc taggagatgt atctattaga cctagttttc
      661 ataaggtagc tataagccta tcggatgaag atggagtcat gaaatacaag aaaattgtag
      721 atgaaataca caattctggt gcaaaggtat cagcacaact attttgttca gattatgatg
      781 ttaatttaat taaagatact atgaatatgg gaattacttc acatgatgag ataaaaaaaa
      841 taatgaatga cggagtaaaa gattacataa caaatatgcc caaagaagaa attaaaaata
      901 ttataaattt atttaaagta acagcattga atgcaaaaaa agcaggtttt gacatgattc
      961 aaattcatgg tgataggctt gtaggaagtt tttcatcaag tatatttaat aatagaaatg
     1021 atgaatatgg tagaacgtgt gataatagaa gtagatttgc aagcgaaata attagtagta
     1081 tccgagatga agttaaggac attccaatag actataaatt tgcaataaga caagaaaatc
     1141 ctcattatgg aaatgcagga gttttacttt cggaagttga gtattttgtt aaaaaatttg
     1201 aatccctagg tgtaaacagt tttcatgtaa cacttgcaaa tcattcaaaa ttagaagata
     1261 ctataccaac caataatcat ccttacttta aagaagaagg ctgtttttta tatttagctg
     1321 atgaagtaaa gaaacacaca aatcttccag tatgtggggt tggaaaattg tctagcccag
     1381 attttataga aagtattatt tcaaataata gagtagatat ggtttctatg tcaagacaat
     1441 tactagcaga taggaattgg ttacagaaag taaaagatgg aagagtagat gagattaaaa
     1501 agtgctgtta ttgtaataag aagtgtgcag atgctttaca aacacgctct cagtttggat
     1561 gtatactaga ttagaatatc cttataaaaa taaataattt gttaaggtta atatattttt
     1621 ggtgatttag ataagtattt agataaagag gaggagacat catgaaaagt ttagttttat
     1681 attcaagttt aactggaaat actaaaaaga ttgcttatac tatatatgat gaaattcaag
     1741 aagaaaaaga cattaaagat gtaaatgaat tagttgatta tagtattgac tatgaaaatt
     1801 atgatatagt attcttaggc tattgggtgg acaagggaat ttgtgataaa aattcaaaac
     1861 aagtgcttga aaatattcac aataaaaaga tagcactatt tggaacaatg ggagcaagta
     1921 aaaaaggaag ttatggagct agtataatag aaaagataga atcaattatc ccaaaagata
     1981 atgaaatatt aggttcattc atatgtcaag gtaagatagc agaaggattg aaagcaaaat
     2041 ataaagagat gttaaaactt tctcctgaca acgaacatat tcgccaacaa ttaaataacc
     2101 atgaggaatc tcaaagtcat ccagatgaac aagaaatata tgaagccagt atgtttgcaa
     2161 aaaatatgat gattaaagct agtattgtgt agtagttaaa caataagttc atataagata
     2221 tagaataaat aatttagtaa aaaagtatag caaataattt gctatgcttt ttttatgaaa
     2281 tcaaaaaaat aataaaaata cttgactgta aaccttgttt atacattatt ttatagaaaa
     2341 taagatagtt ttaaatataa aaaaagatat attattgaaa ggagatatta ttgtatgaaa
     2401 acttataaaa gataattagt ttgattttaa gatatactaa atatacaatt ttatagagtg
     2461 aaaatattta agactatatt atagttataa attaatagaa tagagaggta gtgtttaaat
     2521 tgcaataaca gtatttccaa agttgccacc tcaaattcct atccaatgga ataatggtgt
     2581 agctaattat gtagataaaa aatttattta tgcctaccct gtcatttgtg ttgtattcag
     2641 aatcctttta cgtccattta tatggagatg gttacaaata catgcttttt atagtgacac
     2701 tattgcagat tatttaacga attttatgtg ttttgtagcg ttgtctatag aaatattctc
     2761 tatacttttt atttacaata ttgccagaca tgttacaata attattttta tagatatact
     2821 tgtatt
//
LOCUS       DBGJFU010000236         2812 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.241, whole genome shotgun sequence.
ACCESSION   DBGJFU010000236 DBGJFU010000000
VERSION     DBGJFU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.241"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..1669
                     /locus_tag="ACOIAS_003728"
     CDS             <1..1669
                     /locus_tag="ACOIAS_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HGZ2703392.1"
                     /translation="SLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSVI
                     ICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIGK
                     IQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESVN
                     LIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCIA
                     FEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILLI
                     SYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEID
                     VQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVGE
                     KIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKTI
                     LQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFVW
                     TVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            1991..2575
                     /locus_tag="ACOIAS_003729"
     CDS             1991..2575
                     /locus_tag="ACOIAS_003729"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HGZ2703393.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSLDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1095 a          268 c          435 g         1014 t
ORIGIN      
        1 aagtttacat atagtctacc taaatatgga taattttata aaagttttga ccaatcccat
       61 gtcaatagtt ctgataatac tatcagttat aatactagct ttttatgcat tttttgaatt
      121 tacctctgta attatttgtt ttaataaatc gataaaatat gaaaaaatag ggttatttga
      181 actctttaaa atatcatttg agaactctat aaaattgtta tatcctaaaa atatactatt
      241 atttatattt gtgatattaa taattccatt agtaaataca gttttaattt cagggtttat
      301 aggaaaaata caaataccag agtacatatt agattatata aagtctgata taattttaaa
      361 tataatctat atgatttttc ttttaataat atatatttcg gttattagat ggatttttag
      421 cattcatgag ataactttaa atacagaaaa ttttaaaaga gcaagaaaag aaagtgttaa
      481 tcttataaag ggaaagatta taagaacaat tatttattca ttaatattat ctttagtagt
      541 agctattatt ggatatgcga tttatcatag tggaattata tttataggca tatggactaa
      601 atattgtact gatatggaat ttttgaagaa tgtatttata aatagatgta ttgcatttga
      661 agaatatttt agattgattt tatcaatatt catttttata ttggacattg gatttatctc
      721 agcaacttat tatcaatata aaggagtaaa tatttctaaa aataatatta aaaaagagaa
      781 aaaatctata tcaaaaattg tttttaattt agtattaata ttattgattt cttatataga
      841 gtcagttgca ttttcttcac atataaatgg tatgtttaat acgtcatttt cattttatac
      901 aacagcaatt gctcatagag caggagctga aatggctcca gagaatactt tggcagcaat
      961 agaagaagct tcacatgcta aagctgatta tgcagaaata gatgttcaag aaactaaaga
     1021 tggagaactt attgtacttc atgattcaaa ctttaagaga actacaggag tagataaaaa
     1081 tgtttgggat gtaaattata atgaagtaaa gacatatgat gcgggaagct tttattatta
     1141 tggaaaaggt tattatgatg aaaagtttgt tggggaaaag ataccaacac tcaaagaaat
     1201 gataaaatat tctaaaggta aggtgaaatt attaatagaa ataaaactta atggacatga
     1261 aaagggagac gtagtaaaaa aagttgttca gttaattaaa gaaaataatt ttgaaaatca
     1321 atgtatagtt gcatctatgg ataaaactat attacaaaaa gtaaaaaaac tggatgagaa
     1381 tttactgaca tgctatttta tggcaattgc atatggtgat ttttcaaagt taaattatgt
     1441 tgatatatat ggaattgaat caacttttgt taataaaaca gtagtagaaa agattcataa
     1501 aatgaacaaa aaaatcttcg tttggactgt taatagtgac agtttaattg agaaaatgct
     1561 tgacctaaat gtagatagta ttattacaga taatccatac cttatagaga gtgcaattta
     1621 ttggaagaaa aatggattta ttagtcaagt gtcagattat ttattttaaa tattctataa
     1681 ttgtattatc cagtaaaaga gatattatta aaatcaataa tatctctttt taacataaat
     1741 aaggttgtta aattaaggct attcttataa aaattgtgtt tatcagatat aaataaggaa
     1801 taaaagtaaa aaaattgcta tgacattatt acaattaaaa gagttttaat atgatagaga
     1861 ggtagattgt caactgtata tttaataaat tttattattt attagatttt atataagtaa
     1921 aatatataga tatataaatc ttatgctaga atatatatga aaaacaatat agatttggag
     1981 ggttaaaagc atgacagagc gagaaaaaat gttggcagga gaactttacg attgtggtga
     2041 tattgagttg ttaactcaat ggcataaagc aaaagaccta gtaagagatt ataaccaaac
     2101 agattccttg gatttggaaa aaaaagaaaa aatattaaat gagttacttg gtggtaaggg
     2161 taaaaactta tggattactg caccgtatta tgtagattat ggaaataata tttattttgg
     2221 aaataattgt gaagtcaata tgaattgtac gtttttagat gataataaaa ttatcattgg
     2281 ggataatgca ctaatcgcac caaacgtaca aatatatact gcatttcatc caactaatgc
     2341 tggtgataga tttggtaatg ccaaagaaga tggttcattt gaattttgta aaactcaaac
     2401 agctccagtt actattggag ataatgtttg gattggcggg ggtgctatta tcatgccagg
     2461 tgttactatt ggagataatg tagttatagg tgcaggaagt attgttacaa aagatattcc
     2521 aagtaatatg attgcatacg gaaatccatg ccgtattatt cgagaaaata aataaagtta
     2581 aattttcttg caaaatctca aaaaacacct ttcttacttt atacataggg tattttctaa
     2641 tactttcaca tccatattca tttgttaaat ttttgtggaa tgtagttttt atcacttcta
     2701 tcttacattt ccaccatttt tttataaaag tcattccttt atagatttat ttataatagt
     2761 atatattcta tttgatgttt gaataagcac ttatttttaa gtaaaatact aa
//
LOCUS       DBGJFU010000237         2717 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.242, whole genome shotgun sequence.
ACCESSION   DBGJFU010000237 DBGJFU010000000
VERSION     DBGJFU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.242"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     repeat_region   470..1619
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=470..498
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            2028..2315
                     /locus_tag="ACOIAS_003730"
     CDS             2028..2315
                     /locus_tag="ACOIAS_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703394.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFMTIKIFIFLSLPLCFNILFNYLTS"
BASE COUNT         1041 a          249 c          369 g         1058 t
ORIGIN      
        1 catttaagaa aaatataatg cctacttaca taaaatggaa tgttatttaa agagaacttt
       61 gattatattt tcagaagctt ttttatccat atcgtttaaa acaagaaaat atctattcat
      121 agttattttt atattagtat gtcctaatct ttcagagatg atttttatat tagttccagc
      181 tagaagaaga attattagaa tagataatat agtaagtatt tacaaatatg taggtgttct
      241 taaattgata aattattcca ttttaatttt atagtttgaa ttttatgata taataaaaat
      301 atataaattt tgcagtgagc gatatttttg ataaagtagg gtttaacagt tgcaatgtaa
      361 gggattgagg gtgtgtgata aatgttatca attgcactac tcatggttca ctgcaaattt
      421 gagagagttg tatgtgtgta agtactgaaa atacttagtt tattttgggg ttttatatta
      481 actatgtggt atgtaaatag cagttaaagt tgcaaacatc aacttaacag gaaaagtttt
      541 atattaacta tgtggtatgt aaattccaaa attttaactt tttctttttc taaatcttta
      601 tgttttatat taactatgtg gtatgtaaat tttaaattat aaaaagtata caaacgaaaa
      661 ttatcaacgt tttatattaa ctatgtggta tgtaaattaa cctctcctgt acgtttatct
      721 atgtactcat taggttttat attaactatg tggtatgtaa atctctttag cgcttcttca
      781 agttgtattt gtgtataagt tttatattaa ctatgtggta tgtaaatttc atattccaag
      841 cgttttgctt acacttaata gaagttttat attaactatg tggtatgtaa ataaaattaa
      901 atttcatgct cttagacata catatgcaac gttttatatt aactatgtgg tatgtaaatt
      961 agattgcttt caaattacct cttgtatagc gtcttgtttt atattaacta tgtggtatgt
     1021 aaatcttttt ttattagtga aagggggtgg ttaaaattaa agttttatat taactatgtg
     1081 gtatgtaaat ttgctccgct atgccttgcg ctctctaata tcgcttagtt ttatattaac
     1141 tatgtggtat gtaaatggtt ttaaagaccc agcaacgata aaacaatatg ttgcaagttt
     1201 tatattaact atgtggtatg taaattgaac tttaatcggt tttatacaac aatataacgc
     1261 gttttatatt aactatgtgg tatgtaaata atgaaggtat aataaaatta acagaagaaa
     1321 caagagagtt ttatattaac tatgtggtat gtaaattcac acatgagcaa tatcgaaaca
     1381 ggcaaatcta caggttttat attaactatg tggtatgtaa atttcgtaaa ataacaatta
     1441 tctggaatat gagaagttgt tttatattaa ctatgtggta tgtaaatcac tcttaagttg
     1501 tgacctatct gatatatgat taaagtttta tattaactat gtggtatgta aattcaatat
     1561 ctatattaag tttattttta aaaacatctt gttttatatt aactacgtgg tatgtaaatt
     1621 tgcaacaagt ataggtaaaa taccccaata atttatacag cattttatcc tttaaaatat
     1681 aattattttt tagcatttgt agtaaataat taccagataa cattgacttt agttttaatg
     1741 attaaaatat aaaagtagaa taattataaa aagtattgaa aaatttataa atatatataa
     1801 taaaacttaa tgacaagata ttagatataa aaaataatta ccttataaat agattgaaat
     1861 ttatgaatat tcatactata atttaaatat aaggagatgc cctttgaaaa taaaaattaa
     1921 aaaatattta atgctactac aataggaact agaactacac ttaataaata tacagaaatt
     1981 agatttggct caatataaaa tacaaatata gaatttaggt gttttttatg aaaaaaattt
     2041 tatatgcttt atatagtttc attgttatta tagctaattt tagattaaaa gaaaaaaatt
     2101 ataactttat tttattagca atctttttta tattgctgtt aattcaaaat ggagttttaa
     2161 aaacaggtta tcaaaataga gctacatttt atcaagataa ttattttaat ggagaggata
     2221 aaaatgataa gataaaattt atgactataa agatttttat atttttgtca ttaccattat
     2281 gttttaatat actattcaat tatttaacct cttaaattat atttagatat aggagtctta
     2341 tgataaaagt aagcttatgt gttatttgta gtaaatataa agtgcatata gtgataaaac
     2401 aaaagtttca tataaatgaa gaaaaaaata taaaataatt tatttaacta cttaaaaata
     2461 aactaaaagg gataataaaa tagcttttag tttatggtaa tagaggtatt taaaaagagt
     2521 gcataagcac tccaattgtg agaaatacat acacaaatca ttattacaac aatgaaaaat
     2581 gtacatataa gagacgtttt gtatttgttt taagtgataa tattttaata ctaactaaat
     2641 aattatcaca atggtaaaaa agaaaaagga gtattttgta ctccatttgt caaaaatata
     2701 aaacttttat ataagct
//
LOCUS       DBGJFU010000238         2685 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.243, whole genome shotgun sequence.
ACCESSION   DBGJFU010000238 DBGJFU010000000
VERSION     DBGJFU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.243"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            102..1322
                     /locus_tag="ACOIAS_003731"
     CDS             102..1322
                     /locus_tag="ACOIAS_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HGZ2703395.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAVVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1359..>2685
                     /locus_tag="ACOIAS_003732"
     CDS             1359..>2685
                     /locus_tag="ACOIAS_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2703396.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKKGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLY"
BASE COUNT         1016 a          340 c          453 g          876 t
ORIGIN      
        1 ttcaatattc ttataaaaaa caaaattctc catcgaaaat tatggcataa taattgctta
       61 ttatattaat aaaacaataa aaagcaattg gaggaataaa aatgcaacag gctttgaaat
      121 taaaatatca aaccaaagaa ttaggaaaag attttgtaat agaaagttgt aattctataa
      181 aaccatggct tataaacata agaagagaat tacataaaat tccagaatta gctcttgaag
      241 aaaatcttac taaacagaag gtaatttctt atttaaaaga aatcggaatt gattatatgg
      301 aatttacaaa acacaatgga attatggcat atattttaaa agaaagtgca gataaaacga
      361 tttgtataag agcagatatg gatgctcttc ctattgaaga agaaaacaac ataccttata
      421 aatcaataca ttctggaaaa atgcatgctt gtggccatga tgcacataca accatgctac
      481 ttggagcatg taaagtttta cattcaataa aagataaact taatgtaaat gtaaaattct
      541 tatttcaacc agcagaagaa ggttttggag gagcaaaatt tttagttgag gatgggtgtt
      601 tagaaaaccc caaggccgat tatatattcg gacttcatgt tatgccacat atagaaacag
      661 gccttatcga aacaaaatat gatacattaa atgctagtgt agacactata aaaataagta
      721 tcaaaggtaa aagagctcat ggtgcatatc ctgaaaacgg tattgatgca atagttacag
      781 cttcacaaat tgttacatca ttacaaacta taataagtag aaacttagaa ccaaataatg
      841 ctgttgtttt gactataggt aaaatatatg gtggtgatgc acacaatgta atatgtgaag
      901 atgtaaaatt agaaggcact ttaagaacct taaatagcaa aacaagaaac tttatgattg
      961 ataaaatagc taaaattgtt ggagatactg catctgcatt tggttgtgtt gggactttac
     1021 atgtaagtga tgaaaactat cctgctgtta taaatgaaaa agaattagta gatacagtta
     1081 tatcaaatac taaagaatta cttggagagg aaaaatttat attgagacca aacccgtctt
     1141 taggtggtga agacttttct ttttacacag aacattgtaa aggagcattc ttccatttag
     1201 gatgtaaaaa tgaggaaaag ggcttaattt cacctcttca tacctctagc tttaatatag
     1261 atgaagattg tttaccaata ggtgttatga tgcatgtaat gaatactttg tattttaatt
     1321 agtacagtaa ttaataaaag aataggagat aaattaatat ggaaaaagct aagaaattag
     1381 gtctatttag tatgatatta ttagggatta atagcataat aggttcaggt atattcttac
     1441 taccaggtaa agtttataat ctagcaggtc aaaacagtat gtttatatac atttttgcta
     1501 cactcttagt tctatcaata cttttatgtt ttgcagaagt tggaagcatg tttgataaga
     1561 aaggaggagc atatctatac tccaaaaaag cttttggaga ttttatagga tttgaagttg
     1621 gtacaatgtc ttgggttatt agaattatat catggtctac tctagcagta ggatttgcaa
     1681 cagctttagg ttcattttgg ccagaatctg ccactgaata caaaggttat atagctgcaa
     1741 tactcgtaac actactttca ataaatagct tatttggaat taaaagcaca aagataatga
     1801 ataatgtaat aactatagct aaattagtac cattaatagt ttttataata gttggaatat
     1861 tttttataaa atttgtaaat attgttcctt caggaaatgt agttaactct agtatgggtc
     1921 cagccattat attagtattt tacgcattta ccggctttga atcatttatt gtggcaagtg
     1981 gagaaatgga aaatcctaaa aaaaatcttc cagtcgcatt aataaccaca atatttatat
     2041 gtgcaataat ttatatatta attcaaattg tctgtatggg tatacttgga gatagactat
     2101 ttgaaaacag tattccaatt gcagatgctt caagtgtatt tgtaggcaat tatggtaaag
     2161 ttttcatatc tgtagcaaca ttaatatcta tatttggtat aaatataggt tcatcaatag
     2221 taactcctaa atgtggttct tctctagcag aagaaggttc tcttcctgca tttataggaa
     2281 aaacaaataa atatggtgct ccatatgtag caataataat atctttaatt tgttgtatac
     2341 cacttgtgct aactggaagt tttgaacaac ttgcagtaat gagcgtaatt gcaagatttg
     2401 ctcagtacat accaacttgt ttatcagtaa tagttttaag aaaaagaaca gatgttaagg
     2461 cctcttttaa gattccattt ggccctgtta taccaatagt agctatttta ggtagcttat
     2521 ggttgttaca acaagcttgg gcagaagata tttcaaaacc tataactcaa aatagagttt
     2581 taataggatt aggggcaatg ttgttaatag ctcctctata tatatttatg aaaaaaaata
     2641 aggaaatcga gcataaaaat aaatcagaag attctaaatt atata
//
LOCUS       DBGJFU010000239         2577 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.244, whole genome shotgun sequence.
ACCESSION   DBGJFU010000239 DBGJFU010000000
VERSION     DBGJFU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.244"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(144..2333)
                     /locus_tag="ACOIAS_003733"
     CDS             complement(144..2333)
                     /locus_tag="ACOIAS_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HGZ2703397.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT          825 a          452 c          237 g         1063 t
ORIGIN      
        1 aataatcatt cttataaata aattttcacc atagtaaata tatacagaaa attcttgttt
       61 ttatgcaaat tgattctttt ataaaaataa gtgtcataaa gaaatgtctg aaatcacttt
      121 atgacaccta tcataataaa aaattattct ggtaatgctc ttttatttac ttttccgatt
      181 ggtgtaactg gtatcttatc tatcctcatt attattgttg gaatcatata tggaggtaga
      241 tgctcattta aatattctct aatagttctt tcatttatct taccactatc ttcctcatca
      301 attacataat atgcacatat atacatatct ccatcatcat ttgttctcga tatacaaaca
      361 acacttttaa tttctgtaat attatttatt atattttcaa tctcatatag ctctatccta
      421 aactcatcaa tttttacctg attgtccatc cttccaatat aatctaaatt tccatcatct
      481 aaccatctta ctaaatcacc agttctatac attaacttat tatgttcaca agttttgtaa
      541 ggattctcta caaatacttg ctctgttagc ccttttttat ttaaatatcc tcttgatatt
      601 tgaatgcctg atagacatag ctctcctggt gctccaattg gacatagttt gttcttttta
      661 tctaatacat atgcataagt attaaatata ggttttccta ttggtatttt tccatattct
      721 ctgtccacat gaaaagaagt cgatacaaca gtaaattcgg ttggtccata ctcattatat
      781 agttcataat tacgctttct atatcctttt aatctttctc ctccggtctg tacaactcta
      841 agtgaattat tattgcaatt ttgtataaat aaccttgcaa atgctgttgg caatgctagt
      901 atagttatgt cattttcctc acagtattca tttactgtat attcatcaaa tttagcttct
      961 tcaggcataa tatctattga tgcacctaca agcaaataag ggaatagttg tttcattaca
     1021 gcatcaaaac tgaaacttaa gtatatacca catatatcat tttctgtagc gcttatagat
     1081 tttacgctat aataacacat atttactaaa ctcttatgtt ctatagctat ggtctttggc
     1141 ctaccagttg accctgaagt agatatcgta tatgcacaat ttgatgatgt tattttttca
     1201 gttaattcat atccaagctt aaacatttct tcatcattta tatcgagtac tatggtattc
     1261 tcatttacaa ccttactttt gagttcatct gtagttatca ctacttttgc attactatct
     1321 tcaagaaggt aattcaatct ttcttctgga ttacttgtgt ctattggaaa aaatgctccc
     1381 cctgatttca taatccctat tgctgtaata attatgttaa tatctcttgg taacattaca
     1441 gctactatgt cttcactttc cactcctata tgatttaaat aacctgcaag tttatcagtt
     1501 acttcatcca attctttata tgttaacttt ctattttcat ccctagcagc tatattacta
     1561 ggtgttacct taacttgctc cctaaataaa tcaacataac ttttatcata tggtatttca
     1621 tattgattat tattaaagtc ttccaataat actttttctt cttccttaca tacaaattct
     1681 aaatcttcta ttaaaatatc attatcttct attatttgct ctattatata atcaaattga
     1741 tttaaaattc ttctaatagt ttctctttta tatatagatg gtttatactt tatttttatt
     1801 ttaagtgccc tcttagtata tactattaca gataaatcat atcctttttt ttctatgtca
     1861 taatcatatg ataaatcaaa acttttagct ataaatattg tgtttataag cttagtattt
     1921 agatgatttt tattctctat atctgataac aaatagtatc catactctaa agacctgctc
     1981 cactgccttt ttagcttcct tacaacatct ataaattttt cactttgtct aactcttact
     2041 cttattggaa ttatattcat tgttttacca aaaactacat catcttcata tatatacttt
     2101 tgaagtataa ggccatatat agtttctatg atagagtcta tgctaatatt gttcacttta
     2161 gaatattgaa ttaacctttc atgtttctca atattcatct taaaaacctc atctactact
     2221 tcatcagaac aatctttata aaaaaaatca tttggaattt ttgctatatt cgtataatca
     2281 tctaacaaat ccatccaata attcatagct cgttctaaat taacttcata catatcaaaa
     2341 ttccccctta ctactaagtc tatagaaaaa attcttttgg aattttattt tcacaatata
     2401 taaatatatt aataatacat atgcaagctc taaaaacatg agcttgcata aaattacact
     2461 tattatgact actaaattcg taatatttac aatgtttaaa atatattata acacatatat
     2521 gattttattt ttctacattt atataatata taaattacaa atagttattt aactaat
//
LOCUS       DBGJFU010000240         2533 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.245, whole genome shotgun sequence.
ACCESSION   DBGJFU010000240 DBGJFU010000000
VERSION     DBGJFU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.245"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            636..2432
                     /locus_tag="ACOIAS_003734"
     CDS             636..2432
                     /locus_tag="ACOIAS_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HGZ2703398.1"
                     /translation="MKPTMEILTKLQENSKKNHDEVFTRLYRYLLRPDIYYVAYQHLY
                     SNKGAGTKGVTDDTADGFSEIYIENIIEALKNEMYQPKPVRRTYIKKSNGKMRPLGLP
                     VFTDKLIQEAIRMILEAIYEPIFSDYSHGFRPARSCHTALAQIKKEFTGARWFIEGDI
                     KGCFDNINHAVLVEIINQKIKDARFLKLIRSFLKAGYMEDWKYHKTYSGCPQGGIISP
                     ILANIYLNELDRHVMKIKKEFDVATKARYTPEYTKLVGLRQRLHNKIKNSNGIEREKL
                     IEEYKSATAQMLKLPAKQCDDKKIKYVRYADDFLIAVNGNRQDCEKIKQELTEFISTT
                     LKMELSQEKTLITHSNTPARFLGYDVRVRRDQQIKPKGKFKTRSMNNKVELSIPFKDK
                     IEKFLFSNGIVKQRSDNGKLEPIHRPQLLNRTDLEIVTIYNAELRGICNYYGLASNFN
                     KLIYFNYLMEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTVIKKMYFANYA
                     DCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTESDRYEIHHVNKVKNLKG
                     KSEWEKIMIAKRRKTIVVCHKCHMAIHHGEKK"
BASE COUNT          971 a          433 c          528 g          601 t
ORIGIN      
        1 ttagaagtag attaaagaaa gggggacaga aaaaaaaggc actaccccgt aagataacac
       61 gggaaaccac ctgcctaacc gaaaggtgaa aactgtacgg gaaaatagca cggcaggaaa
      121 gcagaaagtt gttcaaagac aatagagcac gactgaactg caaggttaag tgaatatgag
      181 gataaaacta gatttgttga atgtgagttc caaacctctg ttacgtgtgg acacaggaaa
      241 cttgtctgaa acctgtggta tgagtaacgc tgaaaataaa tgcctttcag tgggttatca
      301 attaactcat acggtacgct ggagaaccag tagtaacgga acgaaagcat atccgacaat
      361 tcacaccagc ctactacttt cgtcaactgg agattaccta aaccagaatg cctacaatgg
      421 ctatgtgtaa tgctcttgta gtgataaatc acaaggctat gctaagtgag atgacgctga
      481 aaatctggca aggtaacgga gctttcatag tagtctgaga accctgaaaa gacagggaaa
      541 gccgtaaaat cggatacggg aaaaccgttc acatggcgaa ggaaagcagt cttagcgaat
      601 taaaattaga aattgattag ggaggaaaac ctcaaatgaa accaacaatg gagattttaa
      661 ccaaattaca ggaaaattca aaaaagaacc atgatgaagt atttacaagg ctctatcgat
      721 atttactccg tccagatata tactatgttg catatcaaca cctctactcc aacaaaggag
      781 caggaacaaa gggtgtaaca gacgacacag cggacggttt tagcgaaata tatattgaaa
      841 atatcattga agccttaaaa aatgaaatgt atcaaccaaa accagtaaga cgtacataca
      901 tcaaaaaatc aaatggcaaa atgcgtccct taggactgcc tgtctttact gataagctga
      961 tacaggaagc aatacgcatg atattagaag caatctatga gcctattttt tcagactact
     1021 ctcacggttt ccgaccagca agaagttgtc atactgctct tgctcaaata aaaaaagaat
     1081 ttacaggagc acgctggttt atagagggcg acataaaagg gtgctttgat aacatcaatc
     1141 atgcggtact tgtggaaatt atcaatcaga aaattaaaga tgcacgtttt ctgaaactca
     1201 ttcgttcatt cttaaaggca ggttatatgg aagattggaa ataccacaaa acatatagcg
     1261 gttgccctca aggtggcatc atttcgccta ttcttgcgaa tatctacctt aatgaattag
     1321 accgtcacgt aatgaagata aagaaagagt ttgatgtggc aacaaaggca cgttatacac
     1381 cagaatatac aaaactggta ggactacgcc aaagattaca caacaaaatt aaaaatagca
     1441 acggtataga aagagaaaag ctgattgaag aatataaatc tgcaacggct caaatgctta
     1501 aactaccagc gaagcaatgt gacgacaaga aaataaaata tgtgcgttac gctgatgatt
     1561 tcctaatagc tgtgaatggc aatcgacagg attgtgagaa gattaagcaa gaacttacag
     1621 aatttataag taccacatta aaaatggaac ttagtcaaga aaaaactctg attacacaca
     1681 gtaatactcc tgctcgtttt ctcggatatg atgtcagagt acgccgtgac cagcagatta
     1741 aaccaaaagg aaaattcaaa acccgttcta tgaataacaa ggtggaattg agtattccat
     1801 tcaaagacaa gatagagaaa tttttatttt caaatggaat tgtaaagcag aggtcagaca
     1861 atgggaaatt agaacctata caccgaccac aactgcttaa taggacagat ttggaaatag
     1921 tgactattta taatgcagaa ttaagaggta tctgtaatta ttatgggttg gcaagcaatt
     1981 ttaacaagct aatctatttc aattatctta tggaatatag ctgtttgaaa acacttgcag
     2041 gaaagcaccg ttccaaagtt tctaaaatta gagctatgta taaagacggt acaggcaaat
     2101 gggctattcc ttatgaaact aaaacagtaa taaagaaaat gtattttgcg aattatgctg
     2161 actgcaaagg aaagaagttt actgatattg ttccacaaac agctaaaaat tattcccatg
     2221 acgtaacaac attagaaagc cgattaaagg cgaagatatg tgaagtatgt gggtgtacgg
     2281 aaagtgacag atacgaaatt catcatgtaa acaaggttaa aaaccttaaa ggaaaatcag
     2341 aatgggaaaa gataatgata gccaaaagac ggaaaacaat cgtggtatgc cacaagtgtc
     2401 acatggctat acatcatggc gaaaagaaat aacctgtaac ggagcaaaga tggagagccg
     2461 tgtgcattga gaaatgcaag cacggttcgg ggagaggact gcacaaacct accctagaaa
     2521 taaggaaagg cgg
//
LOCUS       DBGJFU010000241         2510 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.246, whole genome shotgun sequence.
ACCESSION   DBGJFU010000241 DBGJFU010000000
VERSION     DBGJFU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.246"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(124..2205)
                     /gene="bclA1"
                     /locus_tag="ACOIAS_003735"
     CDS             complement(124..2205)
                     /gene="bclA1"
                     /locus_tag="ACOIAS_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HGZ2703399.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATGPTGNTGVTGVTGATGPTGATGVTGEIGPTGATGPTGEIGPTGATGPTGATG
                     VTGEIGPTGVTGPTGEIGPTGATGPTGVTGSIGPTGATGVTGEIGPTGPTGVTGSIGP
                     TGATGPTGNTGVTGVTGPTGATGPTGVTGEIGPTGATGPTGVTGEIGPTGATGPTGPT
                     GNTGATGSIGPTGVTGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNN
                     GDAITGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFP
                     ILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATL
                     TIFRIADTVMT"
BASE COUNT          560 a          564 c          400 g          986 t
ORIGIN      
        1 atgaatatat tttattatta ttgttatttt attcattcta aaataggtaa aactcaatat
       61 ataaaaatac ttttgtcatt taaataaaac ctaaatagtt tttaagatta ttttagacac
      121 gttttaagtc ataacagtat cagctattct aaatatcgtc aaagtagccg cagttcctgc
      181 tgattgccca tttcgtatcc ctactgttgt agatgaaaag ttatttattg taaaagtagt
      241 cccaccaaat aaaaaaccag caaaatatcc agaaagtact cctccgcctc ctggctcagt
      301 agtaagccct gccaaatctt ttcctcctaa aattgggaat aggatagcga aagaatattg
      361 atttatactt gatgaacctg gttgtaatgc tactgaaact gatatataat acacaccatt
      421 ttcttgtact gtaaacgtac catttgcatc tactgttata cttgaactat ttcctattat
      481 tgtttgccaa cctgttatag catctccatt atttagtgtt tgtggagatg gtgcatttac
      541 gaggaagtta gcaactggct gagaggaatt gcctgttgct cctgttggac ctgttgggcc
      601 tgttactcct gtcgctcctg ttgctcctgt tggacctgtt actcccgttg gccctatact
      661 tcctgtcgct cctgtatttc ctgttgggcc tgttggtcct gttgctcccg ttggtcctat
      721 ttctcctgtt actcctgttg ggcctgttgc tcccgttggt cctatttctc ctgtcactcc
      781 tgttgggcct gttgctcccg ttgggcctgt tactcctgtc actcctgtat ttcctgtcgg
      841 tcctgttgct cccgttggtc ctatacttcc tgtcactcct gttgggcctg ttggtcctat
      901 ttctcctgtc actcctgtcg ctcctgttgg tcctatactt cctgtcactc ctgttggtcc
      961 tgttgctcct gttggtccta tttctcctgt tgggcctgtt actcctgttg gccctatttc
     1021 tcctgtcact cctgtcgctc ctgttgggcc tgttgctccc gttggtccta tttctcctgt
     1081 tgggcctgtt gctcctgttg gccctatttc tcctgtcact cctgtcgctc ctgttggacc
     1141 tgttgctcct gttactcctg ttactcctgt atttcctgtt ggacctgttg ctcctgttac
     1201 tcctgtattt cctgttggac ctgttgctcc cgttggtcct atttctcctg tcactcctgt
     1261 cgctcctgtt ggacctgttg ctcctgttgg tcctatactt cctgtcactc ctgttgggcc
     1321 tgttactccc gttgggccta tttctcctgt cactcctgtt gggcctgttg ctcccgttgg
     1381 gcctatactt cctgtcactc ccgtatttcc tgtcggacct gttactcctg ttggtcctat
     1441 acttcctgtc gctcctgtac ttccggttgg gcctgttact cccgttggcc ctatacttcc
     1501 tgttgctcct gtatttccgg ttgggcctgt tggtcctatg cttcctgtcg ctcctgtact
     1561 tccggttggg cctgttactc ctgttgggcc tgtacttcct gtcgctcctc ttggtcccat
     1621 tggacctgta attctaattg gaagacactc acatttagga taacaattat ctataattct
     1681 atcttcaaat gttacaagta tatacggttt gttacaacct ctactagatg taaattcaag
     1741 aatctgataa gggtaattta aagataaagc taacccataa ttaggatatt tattcattgc
     1801 ccatgctttt atagttcccg taatatttaa acaaatatag ttggatgtat cacttatccc
     1861 aattggtaaa tatcccctga tattttccat agacactcta tcattccatg ttatagttcg
     1921 taaattataa tattcactta ttcttttaat ttcaatatct actttttctt taaaaatatg
     1981 attcttattt gaatcaatat gaataaataa ctctgctttt aatatacttt tgtcttttaa
     2041 tatatctggt attctcactt taaaaaaagt aatcaacttt tcttttacaa aactatttga
     2101 acatttactt cctattaagc aataatctaa attactaaag tttttatctg gatatttttt
     2161 agatataaaa gtatcatcat ttaaataaag tataatattt ctcatttata atccctccat
     2221 tttgggtcta tatcatacta tgtttaaaat acatgcttag ttacaataca attaaaattt
     2281 atagtattaa atttttatat aataatgctg atttctacaa aaaaagaaca tgtcattaga
     2341 aatttctaat agcatgttct ttttcattat tatatattac actttaaatt attccttgac
     2401 tggcttagct ggtgtaaact aaaccagtct ttctaaaata ttaagcactt ttttcttatt
     2461 atgtttttta ggatttattg tctatattat tgtaaaccaa agttataaaa
//
LOCUS       DBGJFU010000242         2342 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.247, whole genome shotgun sequence.
ACCESSION   DBGJFU010000242 DBGJFU010000000
VERSION     DBGJFU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.247"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            38..955
                     /locus_tag="ACOIAS_003736"
     CDS             38..955
                     /locus_tag="ACOIAS_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotA family spore coat protein"
                     /protein_id="HGZ2703400.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            complement(1097..2278)
                     /locus_tag="ACOIAS_003737"
     CDS             complement(1097..2278)
                     /locus_tag="ACOIAS_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGZ2703401.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
BASE COUNT          757 a          397 c          304 g          884 t
ORIGIN      
        1 tatacattta aattaaaata taaaaggagt gtatttagtg gaaaataata aatgtagaga
       61 ggactttaga tttacacaag aatatgagga agattatcca aatacaaatg aaagatacta
      121 tgaaaattat caagtagctg atagatacta taattatcca aataaatata aagaacctaa
      181 aataaaacaa tgttgttgta aaaaaagtat gagagaagca ttagaacttc taagatatga
      241 tgctctaaga ccttttgtaa actttaatca atttgctttt atctcagatt tctttatagt
      301 aggtgctaat ttggtaggta tagatctttc agctcctcca aaagataatt tatctggact
      361 tgatggtact tttgaaagat tttctgcttg taactgtgat ttaatagata tagctggtag
      421 agtatcttat cctattccag tccctttaac tcttgaggga ttaattaata ctataggaac
      481 tataccagga gtagctgaat taattgcact tattgatgca gttattcctc ctacgataga
      541 ccttggggct atattagatg caatacttgc tgctataatt gattttatac ttgctgcatc
      601 tactccatta gcaaacgtag atttagcatc attgtgtaat cttaaagctg ttgcatttga
      661 tattacacct gcagattatg aagatttcat agcatcttta ggttactatc ttgataaaaa
      721 acattacaaa gaatgtaatt gcaactgcga ttgtgatgat tgctgttgta ataaaggtat
      781 cctagataat ctttatatgt caaatataaa taatcaagtt actgtagtag ctggtagttt
      841 ggttctaact ggtgttgaag ttctaggtaa gaaaaatgat gttatagtac ttggaaattc
      901 taatgattca agaatatact ttgtatgtgt agattctata gattatattg cataagaatg
      961 attttaaaga gttatatcta aatataaaat agaatctatt ttctaaagta taaaagattt
     1021 aattattact aataaaaaag agtgtctata attatttaaa aatttcttta gacactcttt
     1081 tttattttta tactatttat ctaacttttt taccaagttt aaatactgct aacacatctg
     1141 caagtacgtc tatattctct actggatttc catttacaac tataacatct gctttctttc
     1201 ctttttctag agttccataa tttttatcaa ttcttaaaag ttcagatgct gtttttgtag
     1261 cagcaactat tatatccata ttatccatac cataatctga cattagtttc atttcataat
     1321 atgtttttcc atgttcatta taagaagttc ctgcatcaga accaactgca attttaattc
     1381 cttttttata tgcttttagg aatgactctt tatgtggaat ggcaacaatc tcacactttc
     1441 taagagtagc ttcagttatt ccactttctt ttcctgcatt tagaataaaa tatggtgcag
     1501 ccacagttgg aactaagtaa gttcctcttt caagcatcaa atcaattatt tcatcatcta
     1561 aataaacacc atgttctata gaagtaattc ctgcaagcac tgcatttttt ataccagttc
     1621 ttccttgtgc atgtgtggca gttattttac ctgcttttat agcttcatct acagcagcct
     1681 tcatttcctc caaagttaat tgtggtgacc ctggctcaac tccatcagtt gttacacctc
     1741 cagttgccat tatctttaca caatctactc cattttttaa aactgttctg gctgattttc
     1801 tacattcatc cactccatca ctttcaatac caaaataata tccatgccct cctgtcatac
     1861 atataactgg acctgaagta actataccag gaccatctat agaccctttg tctataagat
     1921 ttttaagttg tacatccaca tatttgggac aacctaagct tctaatatat gttactcctg
     1981 aatccaaata agcatcaagt tgttttatcg tatttactac aagctctaca tctgatacat
     2041 tatcataata tatctttgga tttccaactg gctccattgt caagtgaaca tgacaattca
     2101 taatgcctgg cataacagtt ttatctaagt aatcgtacac atcatatcca tttaaatcat
     2161 ctcttatatc ttcaatttca actatagtat ctccatcaat aattataccc ttattttgca
     2221 taatactatt tccggttcca tcaattaaat tccctgcttt aatatatttt ttgttcatct
     2281 taaatttctc ccccttttaa gtacaatgta ttatttattt attatttcta atattctaat
     2341 aa
//
LOCUS       DBGJFU010000243         2115 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.248, whole genome shotgun sequence.
ACCESSION   DBGJFU010000243 DBGJFU010000000
VERSION     DBGJFU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.248"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(77..628)
                     /locus_tag="ACOIAS_003738"
     CDS             complement(77..628)
                     /locus_tag="ACOIAS_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGZ2703402.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            819..1781
                     /locus_tag="ACOIAS_003739"
     CDS             819..1781
                     /locus_tag="ACOIAS_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HGZ2703403.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          785 a          235 c          370 g          725 t
ORIGIN      
        1 ataaaaaaga taaatcctaa tcaaaggatt tatctttttg tatagaaaat aattattcta
       61 ttattaaatg gcatggctaa actggataat aatttattat tactgctttc aatgtttttt
      121 ctccttgatt atggtatgta tgaattgatt cagctgaaaa acaaatagag tcattttctc
      181 taagttgata aatagagtca tttacttcta attttaattg accttctata atcataatat
      241 attcctgtga tttttcagaa tgaccaacag atgtataact atgaccttct tctaattcca
      301 tttgaaataa ttcgaagttt ctagttggtg tattgggata ataacaataa agacgataat
      361 ctttattttc tgaaacttgt acatctatat cagtttttga tacaataaaa gtttcatttt
      421 gaggttgttc cagtattgca gtatatggaa catttagtcc acttgcaatt ttccaaattg
      481 tgtttactgt tgggtttgag tctccctttt caatttgaga aagcattact ttacttacgc
      541 cagataattc agcaagttga cctagactta agttacgttc attacgtaat ctatttaaat
      601 ttttagcaat aacagtatta atactcatag tagctcctta aaatgtaaat tattgacaaa
      661 tataatttac gaatgtataa tttatttaac attatttata atttacaaat gtataatata
      721 atgaacgtta aatacactgt acatccgtaa tataaattat actgcataaa gagttgtttt
      781 tcaacggatg atagttgaga gattaagggg gaattgtaat gcgtgtactt aataatgtta
      841 cagaggctat aggcaatact catatgttac atttatccaa aatagcagat gaatttggag
      901 ttcaaggtaa tatttatgca aagatggaat acttaaatcc gggatttagt aaaaaggacc
      961 gtgtagcatt acaaatgatt accgaagcag aaaatgcagg tttattagtt ccaggtcaag
     1021 cagttgtgga attaacaagt ggtaatactg gaacaggact agcaatagct tgtgcatgta
     1081 aaggatataa atttatagcg tgtatgtcaa aaggaaattc tatggaaaga gcaagaatga
     1141 tgaaagcttt aggtgctgaa gtagttcttg tagaccaaat gcctaattca gttcatggac
     1201 aggtatctgg tgaagatttg gcacttgtag aaattgaggc tcaaaaaata gcaaaagaaa
     1261 gaaatgcatt tagagcagac caatttaatt tagaagcttg taatcatgca catgaaaagt
     1321 atacagcaga agaaatgtgg gaacaattgg atggaaatat tgatgtattt gtagattttc
     1381 ttggaagtgg tagtactttt gcaggatgtt caaaaacact taaaaaacat aattcttcta
     1441 tcaaatgtta tgttgttgaa cctgaaacag cagcaattta ttcaggtaaa gaaataactg
     1501 accaagggca taaaatacaa ggtggaggat attctatgga tttgccatta gtagacaaga
     1561 aacttattga tggttatatt caagttacag acgaggaggc aactgatgta gcaagaaaat
     1621 tagctaaatt agaaggtgta tttgcaggat tttcttcagg ggcaaatgta tgtgcagcaa
     1681 ttaagttgtt aaagagcagt gaaaaaggaa aaaatatagt tttaactctt aatgatagtg
     1741 gtttaaaata tttaagtact gatttatatg aagaattgta ataatagcac aatagttatg
     1801 caatgttttt taaattaagt atatagattt aaattataaa aagtatctca atgtaaattt
     1861 tgaaagttat aaaggagata cttttttatt ttgtaaatat ggagtatcct acttataagt
     1921 attaagctat atagatattg atgattagct atgcaataaa aaagagtatg tgtttttgca
     1981 tttatacatc ataacttgga ataatacata ttaatgttct aaatgtattt aaaatatatg
     2041 ataaaattag aacaaataga tacaaggaga taaaaatgca aaagagaatt ttaagaaatt
     2101 ttatatttac gggta
//
LOCUS       DBGJFU010000244         2059 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.249, whole genome shotgun sequence.
ACCESSION   DBGJFU010000244 DBGJFU010000000
VERSION     DBGJFU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.249"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            159..557
                     /locus_tag="ACOIAS_003740"
     CDS             159..557
                     /locus_tag="ACOIAS_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703404.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILFIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    992..1255
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1406..1957
                     /locus_tag="ACOIAS_003741"
     CDS             1406..1957
                     /locus_tag="ACOIAS_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HGZ2703405.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          879 a          187 c          305 g          688 t
ORIGIN      
        1 ttaatactac tttgaaaaat cttaaaaaaa gtaaaagtta tgataataaa aataatatta
       61 aagataataa gatatataag aaacttgaaa aaatttgtaa tttttagtta tatatatatt
      121 ataatgaact agttaaaaat ttttatgagg gggaaaatat gaataagaag aaaaaattga
      181 ttgggatagc tatatctttt ctacttataa tcagcattat aaatccatta caagtagaag
      241 cagaagctat tattgagtct agtgtataca tttcttcaat aaaagagaat tatacatatg
      301 ataatactaa atttaaaact attaaatcta aaagtagaca taaaagtagg aaatataata
      361 aaagtaaagt aaagttgaaa aaacgaagta taagaactaa aaagtatgga tatcaaacta
      421 taaataaata taaaagacaa aacgcaagaa gacataatat cctaacaaaa ttaatattgt
      481 tcataatagt catatttttg gttatagttt tagtagtgct aatatttatt aaaagaaaga
      541 agaaaaaatt ttactaaaaa taattaaaag aatatcattt ttttaatcat tggtatctat
      601 ttaataatat gatgtaatta aaatggagta atattctgtt tcaaaggagg catataaata
      661 caggaatgag tattaaatga tggttaatta agtaaattta ttgataaatg tacttaatta
      721 actttgaaga atttaattcc tcatgaatca aaatataaaa ttacatcaac agttattcac
      781 aaaattaata taagaagaat tagagccaaa gagcagacta ttagaccatt aaagtaatta
      841 ttcatagaat tttgtatatg aaaagaaatg tatagtataa tttacttttt ttataaaaaa
      901 tagattacaa aatatatttt ttataaaaag gtattgacat tttttagaaa aaagaatatt
      961 attattttaa aacaaaattc aatacaataa attaaatcct atgacaagga agagtagtta
     1021 aatttaggtt cagtatagag agctgaggat ggtggaatct tagcaagaaa aaatttgatg
     1081 aatggccctt agaggaataa attgaaatca tagttttgag agtagacttt atcgtcagtc
     1141 gcacgttata gcgaataagg tatgtaagta cctgataatg agatgtatga aaattgaatt
     1201 ttatattaat ttttatataa tttaggtggc aacgcggata atctccgtcc tattagttta
     1261 ggacggagtt ttttatttta ctgaaaattt aaaagataga aggtggtgag aaagatgtta
     1321 tgaatttcaa aagctaaaga tgatgatata gtaggttggc aaaaaaataa attgaaaatt
     1381 aaaaaaatag gaggaagtac aaaaaatgaa aacaaataca aaaaaattaa ctttaaatgc
     1441 aatactttta gcgatgggat tattaataca tcaaatcaca ccagctatag gattaccaat
     1501 gaagccagat gtaccactag caatgttatt tgtaatattg gttttaaata gagatgatta
     1561 taaaacttgt ttgatagcgg gtatagtaac aggaatattt acagcactta catcaagttt
     1621 tccgggaggt caaattccta atgtaataga taaaactctt acagcaaata tagtattttt
     1681 attaatgaat attagttata atatgccttt tataaaaaaa ttagctaaga aaacacaaga
     1741 tactgtagta gttgcaataa taatgccaat aggaactctt gtaagtggaa ctatcttttt
     1801 attagcagct caagctatag ttggtcttcc aggggcttca tttacagctt tattcttagc
     1861 agtagtatta ccagctgttt taataaatct agttgcagga atatttttat tcaagattgt
     1921 tagtctatca ataagaagag ttagttatca agcttaaaca cgaaataata ttttacttta
     1981 taaagcatta taataactaa ataacataag aataaagtag attataacta ttttattctt
     2041 atgttgttta aagatttgg
//
LOCUS       DBGJFU010000245         1997 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.250, whole genome shotgun sequence.
ACCESSION   DBGJFU010000245 DBGJFU010000000
VERSION     DBGJFU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.250"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            34..843
                     /locus_tag="ACOIAS_003742"
     CDS             34..843
                     /locus_tag="ACOIAS_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGZ2703406.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            977..1216
                     /locus_tag="ACOIAS_003743"
     CDS             977..1216
                     /locus_tag="ACOIAS_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703407.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1404..1580)
                     /locus_tag="ACOIAS_003744"
     CDS             complement(1404..1580)
                     /locus_tag="ACOIAS_003744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703408.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1634..1730)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          837 a          182 c          311 g          667 t
ORIGIN      
        1 aaaaataaga tttcggagga gtttattata taaatgaaag attactataa aataggagag
       61 atatctaaaa tatatggaat aggtagagat tcattaatgt attatgaaga aataggcatt
      121 ctaagaccag ttagagatat aaatggatat agaatgtata atatatcaga tatatggaaa
      181 ttaaatttaa taaaagaatt tagaagcttg aactttccaa tgaagaaaat aaaagaatac
      241 ctagatgaca gaagtataga gtctacgaaa aatattttaa atgaagaact cgatttaatt
      301 gataaaaaaa tagctgaatt tataagccat aaggaaaata ttataaaaag attgagttca
      361 attgaaagtg taattcaaaa tacaaaaata gatgagatag aggttgtata tatagaaaaa
      421 agaaaagctt tagaattaaa tgctgatata aagagggatg aagacttcga ttttttaatt
      481 caaaaacttc agaaagaata tgaagataga tttaatatac ttggaaacaa caatattggt
      541 tctgcctttt ctactgaagc aattaataaa ggtatattta atgagtttaa atctgtattt
      601 tgttttctgg aaagtaatga gaaagtatat aatatagttt ttgatgaagg atattatgta
      661 actttaaatt atactgggag taattctaat aataagatat atatggagaa ggtgtttaaa
      721 tttatagaag aaaataatta taagataatt gctgacccta tagaaatcta taagatagat
      781 atacatgaga ctggaattgt agaggagttt gtaacggaaa ttcaagttcc tataactaaa
      841 tagctatatt tcaatggatt agatggtaaa tagaatgaaa aaatgttgat ttgccactgt
      901 gttataaaat cggtagcaaa ttatatttta aattataaga tagcatgaaa aaataattgt
      961 ttagggggtt ataaatgtga aatgttctaa ttgtggaagt gtaaatattg aaaagggaat
     1021 tttaacaact ggtggcatat atgctgaaac tattggatta aaatatagag gagatggaaa
     1081 attagataaa gttataaata aaacttttcc agtacaatct actatatatt cagatttatg
     1141 tttagattgt ggagaaatag ttagaaccta tataaaggat aatactgata ggaattggtg
     1201 taaagataag aaatagaata atttttgaag tatattaata tttgaagttg aattttatta
     1261 aagatacaag tgaaaaagta taaatgggaa gaaattgtta tatggaagaa aactttgcat
     1321 ataggggaga ttagtttttt aatataataa catttttaaa tgtagataaa actaaaaagg
     1381 gttctctaga aaacaatttc tatttaatta tacttactta ccaataccat cacttgatgt
     1441 aaacaagatt ctactacatt ttctccgtac tctagtataa aatcatctaa ctgtgtatct
     1501 ataaaatcac aataatcata taaactactg atataagcag ttaacatgtg tgtacattta
     1561 tccatttcat ctctaaacat atatattact atccccctaa aaaactttca taaaacaaaa
     1621 agaacaatat ctttttggca actggctgac ataactcata gtttatttta taagcgttag
     1681 tccctatagc tttgcgtcac taaatttctc tagttttgcc gatttagttt tattctacaa
     1741 ctaaaataat acactaggat aagttattgt tcaacatact tgtcagaaaa ttgaaatatt
     1801 taacagtatc ttaacaatat gaaatctatt tttcactcaa gaaataaact atacatgtag
     1861 aatatttaat aaaaattata tttagttttg tattgaagtt tttgtattaa aaagtttagt
     1921 tcacaagagt tacttggcta gataatagat gaacaaaaat atcttgaaaa aatatatatg
     1981 aatggcaaaa aacaagg
//
LOCUS       DBGJFU010000246         1986 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.251, whole genome shotgun sequence.
ACCESSION   DBGJFU010000246 DBGJFU010000000
VERSION     DBGJFU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.251"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            84..200
                     /gene="rrf"
                     /locus_tag="ACOIAS_003745"
     rRNA            84..200
                     /gene="rrf"
                     /locus_tag="ACOIAS_003745"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..281
                     /locus_tag="ACOIAS_003746"
     tRNA            207..281
                     /locus_tag="ACOIAS_003746"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Asn,seq:gtt)
     gene            286..360
                     /locus_tag="ACOIAS_003747"
     tRNA            286..360
                     /locus_tag="ACOIAS_003747"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:319..321,aa:Glu,seq:ttc)
     gene            366..441
                     /locus_tag="ACOIAS_003748"
     tRNA            366..441
                     /locus_tag="ACOIAS_003748"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Val,seq:tac)
     gene            447..523
                     /locus_tag="ACOIAS_003749"
     tRNA            447..523
                     /locus_tag="ACOIAS_003749"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Asp,seq:gtc)
     gene            534..608
                     /locus_tag="ACOIAS_003750"
     tRNA            534..608
                     /locus_tag="ACOIAS_003750"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:566..568,aa:Thr,seq:tgt)
     gene            623..707
                     /locus_tag="ACOIAS_003751"
     tRNA            623..707
                     /locus_tag="ACOIAS_003751"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Tyr,seq:gta)
     gene            717..790
                     /locus_tag="ACOIAS_003752"
     tRNA            717..790
                     /locus_tag="ACOIAS_003752"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Gly,seq:tcc)
     gene            801..877
                     /locus_tag="ACOIAS_003753"
     tRNA            801..877
                     /locus_tag="ACOIAS_003753"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Arg,seq:tct)
     gene            887..962
                     /locus_tag="ACOIAS_003754"
     tRNA            887..962
                     /locus_tag="ACOIAS_003754"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Gln,seq:ttg)
     gene            974..1049
                     /locus_tag="ACOIAS_003755"
     tRNA            974..1049
                     /locus_tag="ACOIAS_003755"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1007..1009,aa:Lys,seq:ttt)
     gene            1052..1140
                     /locus_tag="ACOIAS_003756"
     tRNA            1052..1140
                     /locus_tag="ACOIAS_003756"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1086..1088,aa:Ser,seq:tga)
     gene            1158..1248
                     /locus_tag="ACOIAS_003757"
     tRNA            1158..1248
                     /locus_tag="ACOIAS_003757"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1192..1194,aa:Ser,seq:gct)
     gene            1257..1333
                     /locus_tag="ACOIAS_003758"
     tRNA            1257..1333
                     /locus_tag="ACOIAS_003758"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1291..1293,aa:Pro,seq:tgg)
     gene            1419..1494
                     /locus_tag="ACOIAS_003759"
     tRNA            1419..1494
                     /locus_tag="ACOIAS_003759"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1452..1454,aa:Trp,seq:cca)
     gene            1555..1631
                     /locus_tag="ACOIAS_003760"
     tRNA            1555..1631
                     /locus_tag="ACOIAS_003760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Pro,seq:tgg)
     gene            1638..1714
                     /locus_tag="ACOIAS_003761"
     tRNA            1638..1714
                     /locus_tag="ACOIAS_003761"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1672..1674,aa:Ile,seq:gat)
     gene            1718..1793
                     /locus_tag="ACOIAS_003762"
     tRNA            1718..1793
                     /locus_tag="ACOIAS_003762"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1751..1753,aa:Phe,seq:gaa)
     gene            1801..1877
                     /locus_tag="ACOIAS_003763"
     tRNA            1801..1877
                     /locus_tag="ACOIAS_003763"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1835..1837,aa:Met,seq:cat)
BASE COUNT          517 a          452 c          531 g          486 t
ORIGIN      
        1 taactctaaa aaaaaataaa aaaagtattg acaaaaatag taaaaaatgt taatattata
       61 cttgtcctag caacaagaaa aaatgtggtt ataatagcgg aggagataca cctgtttcca
      121 ttccgaacac agaagttaag ttctctagcg ctgatggtac ttggtgggaa actgcctggg
      181 agagtaggac atagccacgt gaatgttcca aggtagctca acggtggagc aaccggctgt
      241 taaccggtag gctgtgggtt cgagccccac ccttggagcc attatggctc gttggtcaag
      301 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      361 tttgtgggat catagctcag ctgggagagc acctgcctta caagcagggg gtcacaggtt
      421 cgagccctgt tggtcccacc atttgcggcc tggtagttca gttggttaga atgccagcct
      481 gtcacgctgg aggtcgaggg ttcgagcccc ttccaggtcg ccaaaataaa aatgctggtg
      541 tggctcaacg gtagagcagc tgacttgtaa tcagcaggtt gtaggttcga ttcctatcac
      601 cagctccatg taaaactaat acggaggatt tcccgagcgg ccaaaggggg cagactgtaa
      661 atctgttgtc atcgacttcg gtggttcgaa tccaccatcc tccaccaatt aaatatgcgg
      721 gtgtagctca atggtagagt tctggccttc caagccagct gtgagggttc gatccccttc
      781 acccgctcca aagaaagcgt gggtgcatag ctcagctgga tagagcaacg cccttctaag
      841 gcgtgtgtcc ggggttcgaa tccctgtgcg ctcaccacat atatattggg gattagccaa
      901 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      961 caaataaaaa tatggtctat tagctcagtc ggtagagcac ctgactttta atcagggtgt
     1021 cccgcgttcg agtcgcggat agatcaccaa tggagaggtg tccgagtggt ttaaggagct
     1081 ggtcttgaaa accagtgact tcgaaagggg ccgtgggttc gaatcccacc ctctccgcca
     1141 aaaaaatgac tgaatttgga gaagtactca agtggctcaa gaggatcccc tgctaaggga
     1201 ttaggctggg taaccggtgc gagggttcga atccctcctt ctccgccatt caaaaacgag
     1261 gtgtagcgca gtttggtagc gcacatggtt tgggaccatg gggccggggg ttcgagtccc
     1321 ttcacctcga ccaacttaag cttttagttc agttggttag agtgcccaac tcatggctag
     1381 taattctagg gtttgagtcc atgaaggtta ttcttcatag gggtgtagct cagttggtag
     1441 agcgtcggtc tccaaaaccg tgcgccgggg gttcgagtcc ctccacccct gccaaataaa
     1501 aaggacttga aaaaagttgg attttagagt agtataagat agtaaataag atttcgaggt
     1561 gtagcgcagt ttggtagcgc acatggtttg ggaccatggg gccgggggtt cgagtccctt
     1621 cacctcgacc aagaaaaggg cctatagctc aggtggttag agcgcacgcc tgataagcgt
     1681 gaggtcgctg gttcgagtcc agctaggccc accatttgcc cagatagctc agtcggtaga
     1741 gcaggggact gaaaatcccc gtgtcggtgg ttcgattccg cctctgggca ccaggaatac
     1801 ggcggtatag cttagttggc tagagcgttc ggttcatacc cgaaaggtca taggttcgac
     1861 tcctattacc gctaccatct aactatatca ataaagaact ttgaaaatta aacagtaggt
     1921 taatttataa acaagaaaca aaccataaag ccagatattt tgataacaat agtatctgag
     1981 cctgat
//
LOCUS       DBGJFU010000247         1906 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.252, whole genome shotgun sequence.
ACCESSION   DBGJFU010000247 DBGJFU010000000
VERSION     DBGJFU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.252"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            420..1100
                     /locus_tag="ACOIAS_003764"
     CDS             420..1100
                     /locus_tag="ACOIAS_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2703409.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKVRSITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
     gene            1547..1831
                     /locus_tag="ACOIAS_003765"
     CDS             1547..1831
                     /locus_tag="ACOIAS_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703410.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          841 a          120 c          290 g          655 t
ORIGIN      
        1 tatatagtgc ctaatgtata acctaatgct cgtagacaat aatgtggata ttatcaaatt
       61 tatttttata atagaaattt taatttatat ctatattaaa ttgttaaaga tataattatg
      121 agaataaaaa atatagcctt gaattatata attgtatatg cttatcttta tacagtaaat
      181 aaaaaatcaa atgaaatacc aaataaagtg tatatcttaa aacaatttac tagagataca
      241 aggatgctat gtgaattttg aagtgtgcag tttagataat aaatatatat aaataataaa
      301 gaaaatggat ttatagtatt taaaatttct tataagggta aaaaatgcta ttagaaaatg
      361 tactaaaaat atttttccaa ttctttatag aaaaataatg atatttagga gagattgtaa
      421 tgcaaattga tgtattgata attgatggag ataagaatag ttctcaagag ctgaaaaatt
      481 ttttagaagg aaaggggata aatgtctatt tagcatataa ttgtaaagat gctattataa
      541 aaatatttag caagaaattt gacttaatat ttttagagat aattttagtt gatggtgatg
      601 gttgggattt ttgtaaaaaa gttagaagta taactacttg cccaattatt catataactt
      661 atataaatga aaatcaaagt attttaaatg ctttaaattc tggaggagat gattatttaa
      721 taaagccttt aaatttagaa atattatatg cgaaagtgaa agctatttta agaagaagag
      781 atttattttt aaataataat aaaaataata taaaactaga agattataat agagctagtg
      841 gagtaataaa gttagaaaat aaaatgataa agttaactcc aacagaaaat agattattaa
      901 attactttat agagaatgct gaaaagactc taactattaa ggagatatat gaacatgtat
      961 ggatgaatga gtatttagat gataattata gtgtagtggt tgctgtgagt ggattaagaa
     1021 agaaaataga gaaagattat aaaaatcctc aaaaaattat aacgataaga gaagtagggt
     1081 attactttaa caagatataa agctttataa acagaatgaa tgataaggca tagataatat
     1141 gttagtatat tacaagagtt tttgttctaa cgatatattt gtaaaattag atttaattta
     1201 cttaattaaa ttatagcagt agaataaata taaaaaatgt ctgaaatatt atagacatca
     1261 gtaaaatata taaaactata atataagtcg taataataaa acaaatgaat attaattaat
     1321 aaaaagatag tagctatttt tatatagcta ttattttttt attgttgtgg atgaaaatat
     1381 acttatattt tactaatata catttaaaaa ttacaattta aagcattaaa aaataaattt
     1441 gtattttgtt ttatataaag taatttgaaa aatatggaaa aattgattag tacattatta
     1501 attagggtat aaaatggtgt aaatagaatg ataaggggtg gatgaaatgc ttgtatataa
     1561 taaaagtttt tatcctaatg acatatttcc aagattagat ttttcaaaaa taaaaaaaca
     1621 gttaaaattg atagataatg acctgtcaga ttttggaagc atatgtataa tagaaaaaga
     1681 acattatacg ataagtgtaa acagtatagg tgaaataaat gtgtactatg atttagagta
     1741 cgaaaataag gtgtatagaa tagtttatga gattgaaaag ttatttaaat ctcaagttgg
     1801 aaggtttagc atatctacat acagaaattg ataattaaaa aagtagaaat taaaaaactt
     1861 aatactaaag atatagataa atatatgagg taacagctag agagag
//
LOCUS       DBGJFU010000248         1830 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.253, whole genome shotgun sequence.
ACCESSION   DBGJFU010000248 DBGJFU010000000
VERSION     DBGJFU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.253"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..547)
                     /locus_tag="ACOIAS_003766"
     CDS             complement(<1..547)
                     /locus_tag="ACOIAS_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HGZ2703411.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNK"
     gene            complement(577..1686)
                     /locus_tag="ACOIAS_003767"
     CDS             complement(577..1686)
                     /locus_tag="ACOIAS_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703412.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT          602 a          252 c          216 g          760 t
ORIGIN      
        1 tcttattaaa atctattaat ttattgagta tattatcctc attaattaaa tctaaagttt
       61 tatatctaat aaggtcaaac aaatttaaaa acttattttc agcgttgtct tttataatat
      121 gaggtagtaa atcagaacat aactgtaatc taaaatcaaa atcagcttta ctatctaatt
      181 caccaagata aaaaatataa gtactttgat aagtttcagt ttcaattata ttttgtgtag
      241 ttacattaat atctaagtta tatttagaat ttactataaa aaaaccattg tgttgtatta
      301 tatttatatc ttcaaataaa ttataaaaaa tattttttag atattcaaag tctatatgag
      361 attggtaaat taaataaaag ttgcctttta aattatcgag attattttta tctagaaaat
      421 caataacata aatagcatta ttgaaagtaa ctaaatggct actagcatct aacttttggt
      481 tttcatatgg atagatattt atttttttat taagctgtct ttctaaaaaa tcttttaact
      541 cttccataaa gtctccttaa aaataagatt atattcttat cttatagcta aagtagtttc
      601 tttgtcaaat atgtgtactc tactattgtc aactgaaagt gttaagtcat caccaacatt
      661 aattctgttt ttactgtcaa atctaattgt tatcgtattt tcgcctagtt ttaagtgagc
      721 ataagtttct gctcccataa gttcttttat ttcaattctt gatttaaaac aagtatctgg
      781 attagattta ataaaagtat cttctacatg aatatcttct ggtctgatac ctataacaac
      841 ttcattatta atataattgt cttttattaa atcatagtgt ttttctttat tcaatttaag
      901 cttaatatgt tcattttgtg cataaatttc atcattttct tctattaata ttacatcaat
      961 aaaattcatc tgtggtgctc caataaatcc tgcaacaaat atattagctg gataatcgta
     1021 tacatcttgt ggagttgcaa tctgctgaac tactccatcc ttcattacta cgattctatc
     1081 agccatagtc atagcttcaa cttggtcgtg tgttacatag ataaatgtag ttccaagctg
     1141 ttggtgtagc ttagttatct ctgttctcat agcagttcta agttttgcat ctaagttgga
     1201 tagaggttca tccattaaga ataccttagg atttctaact attgcacgtc ctaaggcaac
     1261 cctttgtctt tgtcctccag ataatgtttt aggtttttta tttaaaagag gtagtaagtc
     1321 taatatttta gcagcttctt taactttttt gtctatttca tctttaggca attttctaag
     1381 tttaagtgca aatgccatgt tttcgtaaac agataagtgg ggatataaag catagttttg
     1441 aaaaaccata gctatatctc tatcttttgg ttctatatca ttgacaagtt tatctccaat
     1501 gtatagttct ccttcagaga tttcttcaag accagcaatc attctaagtg tagtcgattt
     1561 accacaacca gatggtccta ctaaaacgat aaattcttta tctttaatgt ctatatttat
     1621 attttttact gcattaaatc catttgagta aagttttgat atatttttta aaattacttc
     1681 tgacataata tcctccttga tatttaaatt ttaattatat agtatcaaat ttttttatta
     1741 tattaaatgt gtaaaatgac taaatgtatt tgtttaatat gtatagttag tttttatatt
     1801 aagatttatt ataatttaga aaatatattt
//
LOCUS       DBGJFU010000249         1824 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.254, whole genome shotgun sequence.
ACCESSION   DBGJFU010000249 DBGJFU010000000
VERSION     DBGJFU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.254"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(178..735)
                     /locus_tag="ACOIAS_003768"
     CDS             complement(178..735)
                     /locus_tag="ACOIAS_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGZ2703413.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            993..1691
                     /locus_tag="ACOIAS_003769"
     CDS             993..1691
                     /locus_tag="ACOIAS_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGZ2703414.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNIPTKILYGKHDELTEIGVVEKFVEKFNCNLTIVENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          667 a          252 c          222 g          683 t
ORIGIN      
        1 tatgttgttt ctctttatat ctcttctttg taaacaatat tgggcatttg aaaataaata
       61 ttcttaaaat atactgttct cctgttgatt ttcctttggt acaaatgata aaaatttaat
      121 ttacatgccc cataatgaag tgttcaataa ataagttaaa ataatttatt tacctcctta
      181 atcatatttt ttatgaatac ctaataacac agtatcaata gaatcgacta aaccttttat
      241 agcttcttct ttactcaaaa atgatttccc aagttgcaaa gtcgaataac catgaagtac
      301 actcattatt aagtttaata cttcacctgt attctttgta tttagattac aagaaagaat
      361 aagtttttct aataaggatt tatattcatc aaagatttct aatgtttcat tatttccatg
      421 ccaagtagcc cactgaattg tttcataaac accaggatta gaaattataa aattaaagta
      481 tgctatactt atacacttta tagctgtatc tccaaaattt ccaatagccg cctttatcat
      541 ttgactattc atctctctca ttcctttatg agctacttca agtaataaat catctagact
      601 ttctatatga ttgtacaaag atggtgtacg tattcctagc ttttctgcaa taacttttaa
      661 agaaactttg ttaagcccat ctttattaac tatatctgat gtagcttgta taactatttc
      721 tctactaaca cgcatgattt tccctctttc atttaaaatt aattacttta ttagtttaac
      781 taatctaatt agtttcgtca attattgtaa tttataaaat tctttatact attataaaat
      841 cttgacagtt taattataat ttaatataat actaattaaa ttagttttaa tactaatata
      901 attagtatta tattaaattc tatattacaa cattatttta tagtttgatt aatttaataa
      961 tcactaatat atcaaactga aaggagattt ttatggaaaa aaaatatttc gaaattcatg
     1021 gagttccttc aatcctttgg ggaaatcctt caagtaaatt atatatttac atacatggac
     1081 aatgtggcaa taaagaggag gctgaagctt ttgctaatat tgcagttcca catgagtgtc
     1141 aagttttaag tgttgatttg cctgaacatg gagaacgtaa aagagaaaaa aattcattta
     1201 acccatggca tgttgttcca gaattagtat ctattatgga atatgcaaag tgccactgga
     1261 atcaaatttc gttatatgca aatagcattg gagcatggtt tagtatgtta agctttgaaa
     1321 aagagaactt aagagagtgt atttttgtat ctccaattct agatatgcaa cagctcattt
     1381 caaatatgat gctctgggca aatgtatcag aagaacagct taaacatgaa ctaattatcc
     1441 caacttcatt tgggcatact ctttcatggg aatatctaca atatgtaaaa caacatccta
     1501 tcactcaatg gaatatacca acaaaaatat tatatggtaa acatgatgaa cttactgaaa
     1561 tcggtgttgt agagaaattt gtagaaaagt ttaattgcaa ccttactatt gtagaaaatg
     1621 gcaaacattg gtttaacaca ccacaagaat taaattcatt atatgaatgg ttaaatactt
     1681 tagttaaata aaaaataatt atattattaa tatgattaaa ttatttataa atgttattta
     1741 attaatttta ttttagcatt attaaaataa ctacataaag aataaattag ttattattaa
     1801 agaaattaaa agtatataga gtat
//
LOCUS       DBGJFU010000250         1805 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.255, whole genome shotgun sequence.
ACCESSION   DBGJFU010000250 DBGJFU010000000
VERSION     DBGJFU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.255"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..92)
                     /locus_tag="ACOIAS_003770"
     CDS             complement(<1..92)
                     /locus_tag="ACOIAS_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HGZ2703415.1"
                     /translation="MKDKWILCPVCGNKTRLKIRADTELKNFPL"
     gene            complement(131..418)
                     /locus_tag="ACOIAS_003771"
     CDS             complement(131..418)
                     /locus_tag="ACOIAS_003771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703416.1"
                     /translation="MSTLIIGFIVAGVLLIAEYLLCTKFRSPLWGGIIPLLILAGTIY
                     VFASGRIPFERPYIFPFVVVNTLFFGDWGTGRDKYKKIKQTEMEKMKAKDI"
     gene            complement(421..717)
                     /locus_tag="ACOIAS_003772"
     CDS             complement(421..717)
                     /locus_tag="ACOIAS_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HGZ2703417.1"
                     /translation="MELDKIPQWILALEQEDATFLKNFALKSGSLKEIAKMYGVSYPT
                     VRLRLDKLIQKIEMCDQQEEEPFQTFIKGLAVDSRIDVETAKIIIEKYKKEKEK"
     gene            complement(806..1711)
                     /locus_tag="ACOIAS_003773"
     CDS             complement(806..1711)
                     /locus_tag="ACOIAS_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGZ2703418.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKSVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQALNTDTIRKDIPVSSAVNGVQIWSVTQADENQFSVIFTVEQLITENGSTKT
                     VRSAYEVTVYVDGSGNMVLTKNPTITSIPVKSGYTPKAKESDGTVDAVTTDEINEFLT
                     TFFKLYPTATAKELSYYVNEGVLKPIGKDYVFSELVNPIYNRIDSQVTVSVAVKYLDQ
                     QTKATQVSQFDLVLEKSGSNWKIIK"
BASE COUNT          485 a          397 c          300 g          623 t
ORIGIN      
        1 aatagtggga aatttttaag ctctgtgtcc gctctaattt tcaacctcgt tttattaccg
       61 caaactggac ataaaatcca tttatctttc aataatgcgt tcctcctatc taagtataca
      121 gttttgtaat ttatatatct ttagctttca tcttttccat ttcagtttgc tttatctttt
      181 tatatttatc cctgccagta ccccaatctc caaaaaataa agtattgact acaacaaacg
      241 ggaaaatata tggtctttca aatggtattc tgccacttgc aaaaacatat attgttcctg
      301 ccaagataag caagggaata atacctcccc aaagaggact tctaaatttt gtgcagagaa
      361 gatactctgc aattaataaa actcctgcaa caataaagcc aataattaat gtactcatgg
      421 ttatttctcc ttttcttttt tatatttttc aataattatc tttgcagttt ccacgtcaat
      481 tcgagaatca accgctaacc ctttaataaa agtttggaaa ggttcttctt cctgctggtc
      541 gcacatttca attttttgaa ttaatttgtc taaacgtaat cgaacagtag ggtaagatac
      601 cccatacatt ttcgctattt cctttaatga accggattta agtgcgaagt ttttaagaaa
      661 agtagcgtcc tcttgctcta aggcaagaat ccattgaggg attttatcaa gctccaaaaa
      721 cacaactcct ttcatattat ctttaacaat attcagtata tcataaataa aattaaagtc
      781 aataagaatt taaacatcaa aataattatt ttataatctt ccagttgctc ccgctttttt
      841 ccagcaccaa atcaaattgt gatacctgcg ttgcctttgt ctgctggtcg aggtacttca
      901 cagccacgga aaccgtcact tgactgtcga tgcggttgta gatgggattt accagttccg
      961 agaatacata atctttcccg ataggcttta acacgccctc atttacataa taggacagct
     1021 ctttagcggt tgcggttggg tagagcttaa agaacgtcgt cagaaattcg ttgatttcgt
     1081 ccgtcgtcac agcgtccacc gttccgtcgc tttcctttgc ctttggcgtg tagccggatt
     1141 tcacgggtat gctggtaatg gtcggattct tcgtgagaac catgtttcca gagccatcca
     1201 catagaccgt gacttcatag gcggagcgta ccgtcttcgt gcttccattc tccgtgatta
     1261 actgttccac agtgaagatc acggagaact ggttttcatc cgcctgcgtg acagaccata
     1321 tctgtacccc attgaccgcc gaagacactg gaatatcctt gcggatggta tcggtattaa
     1381 gtgcctgcaa ttcctttgtc agataaccgc tgattgcctg cgtccttgct tcaatagctt
     1441 ctttgctgtt attccatgtg taataggact tcgcaaagtt cttcacgaaa ttttcaatcc
     1501 cgttggtatc ggtaagtctt aactgtatgg tttctgtttc atgtacggtg tgcatatcaa
     1561 tagcggtaaa attcttatat accccaaaac tcacgcttgc gataagcact acccacaatg
     1621 caatcacaga ttttttatgt gtaccgactt tcacagtacg cactttcttt tctttgacat
     1681 tctctgtctg tttcttattc ttcttaaaca tattttctaa gtcctttctt ttattttact
     1741 cgtcctgcac cgattaagtg ttgttgccag taactattat ttaagtctgc atatcctatc
     1801 ggatt
//
LOCUS       DBGJFU010000251         1762 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.256, whole genome shotgun sequence.
ACCESSION   DBGJFU010000251 DBGJFU010000000
VERSION     DBGJFU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.256"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(233..1561)
                     /locus_tag="ACOIAS_003774"
     CDS             complement(233..1561)
                     /locus_tag="ACOIAS_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGZ2703419.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          643 a          326 c          199 g          594 t
ORIGIN      
        1 aaacacttta aaataaagta ttttaaaata aataattttt tagatgttta attttataat
       61 caaaaaacta aaaataaaat gaatacctta aaataaactt tttagttcat ggaacgctct
      121 ttttttgtaa aattaaacat atctttacca ttttaacaat aaaaaagaga cttatctcta
      181 tttgagacag tctcttttat tttaattatg aagcatttgt aaactacttg gtttatcttt
      241 ttactcctct agtcatataa gcatacactg gtaagcctat agctgttatt acaagacctc
      301 ctatagatat catagtagtt ttcatacctg caaaacacaa ttgattaact acaacaaata
      361 taccacttgc tatagctatt ataggaacaa taggatataa tggaacttta tactctcttg
      421 gtatatttgg atgagtcttt ctaagtttca taacccctat aaaagttaat acatagaata
      481 tccaagtagc aaatacagct aagtcagtta ataaattaaa ttgacctgac aaagcatata
      541 tagatgcaat aacagatact aatgctattg catttgctgg aacttgagca gaatttaatt
      601 tagaaaatat accatacttt ggtaaagtct tatctgtagc taaagtatat gccacccttg
      661 aaccagttag taaaaatcca tttaatgctc caaacactga aattaatata cctacagata
      721 ttatttttcc acccattgaa ccaaatataa cttcagcaac tgctgatgca ggagaagcat
      781 aatttgctaa ttcattagct ggtaataccc aaagatacgc taaatttatt ataaaataaa
      841 cagccataac aactgacaat ccacctatta tagcttttgg aaggtctttt ccaggattct
      901 tcatttctcc agctaaagta ccaacattca tccatccatc aaaagcaaat aatatagcaa
      961 ttaaaacttg acctagtaca ctacccaggc ttaatccttc accaaccata ggtgtaagta
     1021 ttggattatt tccaccacct ttaataaatc caacaaccat tattaaaata agtggtatta
     1081 acttacaaat tgtagaaaca gtttgtatga ctcctccagt ttttgaacct aacatattaa
     1141 gccctgcaac tattactata attccaattg ctattggaag taaaagtgat ggactaccaa
     1201 ttagtgctga tgcttgttct ccaaatataa cacctaatgc tgccatcatc cctggataaa
     1261 ataatacaat ttgcatccat ccagttaaaa aacctaattt ttctccataa atttctttta
     1321 tatataccat cattccacca gtttttggta tcgcaactga tacctctgca gctgtaagac
     1381 cagctgttat agttatgatt ccagctatta accatgccaa tataccaaga cctggagctc
     1441 ctccagttaa tgtatagact gcttgtggtt taaaaaatac gcccgctcct ataacggaac
     1501 caacaacagt tgataatgct gctgatacgc caagagtctt tttcagttca ttacttccca
     1561 tttttttcct cctcattaat cctatttaat tttaatttca tagttaaata agcatatatt
     1621 atgccaatat atagaaaaat agctttatta tttattaaaa taaaataaac tctacttagc
     1681 tttcgtaatc ttttattttt agctatgttt aaatataaaa aaattataaa aaccaaaaat
     1741 ccaaatataa taatacttct aa
//
LOCUS       DBGJFU010000252         1754 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.257, whole genome shotgun sequence.
ACCESSION   DBGJFU010000252 DBGJFU010000000
VERSION     DBGJFU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.257"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            39..296
                     /locus_tag="ACOIAS_003775"
     CDS             39..296
                     /locus_tag="ACOIAS_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703420.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(406..1086)
                     /locus_tag="ACOIAS_003776"
     CDS             complement(406..1086)
                     /locus_tag="ACOIAS_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGZ2703421.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            1346..1645
                     /locus_tag="ACOIAS_003777"
     CDS             1346..1645
                     /locus_tag="ACOIAS_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703422.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT          711 a          201 c          209 g          633 t
ORIGIN      
        1 tagaaattaa aatgaattaa gtaaatggag gaattgatat gataataatt agaagtcaag
       61 ataaaactaa tttaatgcag ataaaccaaa ttaaaataga tggtagtcaa gtttatgcag
      121 tatttgaaag taaaattgat actgtaaaaa taggagatta tgaaaataat acaagagcta
      181 tacaagtgtt ggacaatata caaaacttca ttgaaaatgg aacaaaatat gattatataa
      241 catcaaataa agtcagatac aatgttaata aaatatttca gatgccagct aaatagtgag
      301 tgtgcatttt aatttaataa ctaaagccca ctttcttttg tggtatgaaa gagggcttta
      361 catatttgta aatatcgaag tacacttgtt ttatcaataa attatttaat aaggtataat
      421 ggaccaatta aacaataaaa attctcctaa aacagcaagt attaaatata taatagcttc
      481 atttttgtaa agagaggtaa ttttttcgct tatgtcatca tcaattgtaa tagcaatttc
      541 atcttgaata atatttttat atatagatgg gaaaatatct tttttcttta taagattaaa
      601 taaattattt acacctaccc atataaaata tcctaaaaca gcacctaatg tgttcatcat
      661 taaatcatca atatctgtag ctcttaagtt aaacaattga caaagttcaa ttgctaatga
      721 aaaacctaat ccagttaatg aaacattgat aatatttcta aaatttttcc aaattaaagg
      781 tagtaaaaat cctaaaggca taaacataat tatatttaaa atatatgtta agatgccaac
      841 tgagtcaaaa ggtattagat ttatttcatc aattctaaaa agcccactat accttcctat
      901 atcccatatg ctaccaattc cagctttact aagtgctaga tagatatata ataaaaaaat
      961 atatacccat actaaatgta tcattacatt cttttcaact ttcaaatttt ttcgttttat
     1021 aacgactatt tggtatatta agcatggtaa tataacgcaa ataaatgaat aaaatgtcca
     1081 tatcatttta ttttgtcccc tttcaaattt atgtttttag ttttataagt actatcttta
     1141 tattaaactg taagttttaa aaatctttta ataaaatctt aaaaatttat taatttataa
     1201 aaatatatta ttaagattaa aaaatatata taatcaaagt ttctacaatt atatagttag
     1261 ggatataaaa attttatcgt atgcataaat attataaata tatggaaata ataatagtat
     1321 attttggtaa agtaggagga attttatgaa atggattaaa tggtatagta ttacatgcat
     1381 atgtattttt atagtagtag ctttttatat gtttatattt cctaataaaa tagaaactat
     1441 tgatacttca tctgcatata gctttgttga aaagaaagta ccaaatagtg ctgtgtatca
     1501 aggatacaaa aagaatccag ttgatggtac tactacaatt tattacagct atgataattc
     1561 aactcatata gttagattat cacatccaga agactattca aggaagatta attgggataa
     1621 agtttcaaac atcagatttg attaatcttt aatatttata aaagtctata tatctaaggc
     1681 aaatatatta ttaatcttat tcatagttga gcactttcta ttatagaagt gcctcttctg
     1741 atttatatca taaa
//
LOCUS       DBGJFU010000253         1740 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.258, whole genome shotgun sequence.
ACCESSION   DBGJFU010000253 DBGJFU010000000
VERSION     DBGJFU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.258"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            87..1685
                     /locus_tag="ACOIAS_003778"
     CDS             87..1685
                     /locus_tag="ACOIAS_003778"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HGZ2703423.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMDTLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          665 a          217 c          272 g          586 t
ORIGIN      
        1 ttctttttac aacaaatact cactaccttt tgatataata tttttatcaa ttattattat
       61 attcaagttt tgaaaggagt attaaaatga gtttattagt tttcggacat aaaaatccag
      121 atacagattc aatatgttct gcaatttcat tagcacattt aaagaacaaa ctaggaataa
      181 aagcaacagc ttatgcttta ggagatatca gaaaagaagc taaatatgcc ttggattact
      241 ttaaggtaga tgcacctgaa attttagata atgttagaat acaagttaga gatttaaatt
      301 atgataaagt agtaccttta acaccaactt catctatatt agaggcttat aaccttatgg
      361 atgaaaaaaa tgttaaaaca ctacctgtaa cttatgatga tggaactttt gctggtatca
      421 ttactatgaa agaaatagct aaaaatctac ttcatcaaca ctttacaact atacacacat
      481 cactatcaaa catttgtaaa aaccttaatg gtactattct tgtggactgt ggtgaagata
      541 taaaaggtag agtagtcact ttatcatttg gaatggatac tctaatagag actttaaaag
      601 aaggagacat agctattgtt ggagatagat atgatagtat tgaatatgct attgatacaa
      661 aagttaaact tttaatactt actaatcata cagagatttc aaaagatttg ctagctcttg
      721 ctaagataaa tggagtgtct gttgtttctg ttgaaagtga caattataaa acttctaatg
      781 taataaatca atgtagtttc ttagactcta tagaagctac agaaaattta attacattca
      841 aagaagatga ttatatggat gaaataaaag agattatgct agaaacaaac ttcagatctt
      901 atccaatatt agatgatgat aataagtttt taggtttagt ttcaaaaggt catctattaa
      961 acccatctaa aaagaatgtt gttttagttg accataacga atatgctcaa agtgctgatg
     1021 gaattgaaca agcaaacata gttgaaataa ttgaccatca taaacttggc ggtatttcta
     1081 ctgatgttcc tatgtcattt agagttatgc ctgttggatg caatagtact ataatttatc
     1141 aaatgtacaa agaaaataat gttgagatac cttatgaaat agctgggctt cttttatctg
     1201 ctatattatc agatacatta ttatttaagt ctccaacaac aactgatatg gataagaaag
     1261 cttgtgaaga attaagtaaa atagcaaaag tagacatgga aaaatacgct atggatatgt
     1321 ttaaatgcgg tacttcttta gatgaatata ctatagaaga aatagtaaat atggacttta
     1381 aagaatttaa tatgagtgga catagagttg gtattggaca agtatttacc ttagacatag
     1441 actctatatt tgctaaaaaa gatgagtttt tatcttatat aaattcgact gattatgata
     1501 aattaattct ttcagttaca gatataatca aagaaggttc ttatttaatc tataaagcag
     1561 aagatagctt aattgctaac gcatttggtg ttgaaggtgt tcaaggcaca tttgcaccag
     1621 gtgttgtatc aagaaaaaaa caattagtcc ctaatttaac tactgcaatc aaaaatttta
     1681 aataatataa aatttttaaa taacgaaaac tgtctcaaat ctatatttga gacagttttt
//
LOCUS       DBGJFU010000254         1728 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.259, whole genome shotgun sequence.
ACCESSION   DBGJFU010000254 DBGJFU010000000
VERSION     DBGJFU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.259"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            222..515
                     /locus_tag="ACOIAS_003779"
     CDS             222..515
                     /locus_tag="ACOIAS_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HGZ2703424.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            496..1137
                     /locus_tag="ACOIAS_003780"
     CDS             496..1137
                     /locus_tag="ACOIAS_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HGZ2703425.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFVYSYLMYKISGKNN"
     gene            1381..1713
                     /locus_tag="ACOIAS_003781"
     CDS             1381..1713
                     /locus_tag="ACOIAS_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGZ2703426.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
BASE COUNT          678 a          156 c          283 g          611 t
ORIGIN      
        1 ctttaacaat tatggtgcaa tacacttttg ttaagttaaa aaaattagat aaagtaagat
       61 taaataaaag cgacttaagt aaattgagaa tcttaatatg aaaattttca ataaatattt
      121 agaaataact atttagaaaa aattctaaat agttattgaa ataaaatgga tgttatgtta
      181 tattctattt agatgaaatt ctaaatagcg aggtgatggt gttggggttt ccagaaacat
      241 tcaaagctct ttcagaccct ataagaagag aaatattggt tatgttgagg gagaataaaa
      301 tgtcagcagg agaaattagt gaaaaattta atatgacgaa tgctacaatt tcttatcatc
      361 tatctcaact aaaaaaggca ggactcatat ttgaaacaaa gtataagaac tttattttct
      421 acgaaataaa tatttcagtt ttcgaagagg ttatgctttg gttttcgcaa tttatggaga
      481 agggagaaaa agataatgaa aaaagcgata aataaacaat ttattttatc tactttaata
      541 tgttttatac catttattgt atcaatatat ttttataata gattaccaaa tgaagttgca
      601 attcattttg ataattatgg aaatccagat aattatgctc caaaggttat agcagctttt
      661 ggtgtaccat tgttaatgct atgtatccat ttgtatacat ggtttagact tgagaatgag
      721 cctaagaaag ctaatttttc aaatgttata caaaatttat caaaatgggg agttgctata
      781 ctttcagtaa ttatacaaat aatgatggtt ttatattcaa caggagttag tttggagtct
      841 aatttttata caggattaat tttaggaata atagttattt tatttggaaa ctatcttcct
      901 aaatgtaaac gaaattactc agtaggaata attttaccat ggactcttaa tgatgaagat
      961 aattggaata agacacacca tttagctgga tggatttggc ttattggtgg aatattatta
     1021 attattaatg catttattaa tattcctttt tataatatct ttgtaatctt tgtaattgta
     1081 atactaccat ttgtatattc ttatttgatg tataaaattt caggtaaaaa taactaatat
     1141 aattaatcac tataataatg tttggataag aataatatat ttcagaactg agatagataa
     1201 taaagaaatt actgagattt ctattttagt tctgaaaaaa tcttacttat atgataactt
     1261 attgagattt caaaaaataa tataaattag gctaatgaac ataaaatgaa aatttgtatt
     1321 taatattgaa aatataagta cagtgtgata ctcttataag aaaaaaatag gaggtagaag
     1381 atgaatacta gagaaatgaa agaatatagg ggattttaca ataaaaaatt aagtgaaaca
     1441 gatgagtttt ttagagtatt tggaagcctt gatgataaga cttattcaga aggggctata
     1501 agtaaaaaaa ataaggaatt aatgggacta gctatttcaa ttttaagccg atgtgatgag
     1561 tgtatttgtt atcatattga gggttgtatt aattctggag caagtaaaga tgaaattata
     1621 gaagctataa aaataggtgt aattggtgga gggtctatta catatccaaa cgcaagattt
     1681 gcaataaaaa ttttagaaga atttaatata taattttgat acacgtag
//
LOCUS       DBGJFU010000255         1705 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.260, whole genome shotgun sequence.
ACCESSION   DBGJFU010000255 DBGJFU010000000
VERSION     DBGJFU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.260"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            491..1696
                     /locus_tag="ACOIAS_003782"
     CDS             491..1696
                     /locus_tag="ACOIAS_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGZ2703427.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          629 a          219 c          226 g          631 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taattttttt
      181 atttatcatg tattctttat ctttaatttt taaaacttaa tgctaagtaa acctgtttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatataaa tactatgcta cattttagga
      481 ggattttatt atgagcaaaa aagtattaat aatgtctgct tccactggtg gtgggcataa
      541 tagggctgct ctagctatta aagaagaact tacttccaaa acacttgatg gagagccaat
      601 tgaatgtgaa ataatagata gtttgaaatt agttaataat acaatggata aaataatttc
      661 aagaggatac gaaaaatctg ctatatatac tcccaaagcc tatggaagtg tatatagatt
      721 atctgaaaca aatcttttgt caaaaaatga atttaaagat aatctactaa taacattcat
      781 ggctaaaaaa tttaaaaagc ttattagaag cgaaaaacca gacttgatta taggaacgca
      841 tccttttcca atgattgctc taagtacatt gaaaaaaaac tttaatttac ataataatga
      901 atcaaatgct tacacagagc atttttataa acattataca aataccatta atgttcctcc
      961 gcttatttct gtattaaccg attatactac tcattctaca tggatacaaa atgaaattga
     1021 ttactatatt gttggtcatg aatatgtaaa agaattattg gtgtttgatg gagttgaacc
     1081 gagtaaaatt agaacatttg gaatccctgt tgaaaaatct tttctctctc acagagataa
     1141 agatattgtt ctttctgagt tgaatttatc tcctgataaa ttaactgttt tacttatggg
     1201 gggtagcttt ggggctggaa atataaaaga gacacttgat gaattactgg atactgatag
     1261 agactttcaa atacttgtaa ttactggtaa gaatgaaagc ctaaaagaaa aaatcgaaaa
     1321 aaaacttatg tctcgttatc atgataaaaa tgtatgtgtc cttggataca ctgataagat
     1381 gaatgatatt ttagcttcta tagatgtatt agtaagcaaa cctggtggac ttactacaac
     1441 tgaggctctt ttaaaagatg tacctatgat agttccttat tatataccag gtcaggaaga
     1501 agagaatctt gatttcttat ctaattgtgg tgcagcactt agaactacaa aaaaatataa
     1561 cttgtcaata ttactaaaag ttttaataga tgaccctagc agattagaaa tgctaaaaag
     1621 aaatattaaa tcaataagaa agtcaaattc agcccaaaat atagctaatt taattttaaa
     1681 tataatttct ggttgataaa ataaa
//
LOCUS       DBGJFU010000256         1610 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.261, whole genome shotgun sequence.
ACCESSION   DBGJFU010000256 DBGJFU010000000
VERSION     DBGJFU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.261"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            381..1112
                     /locus_tag="ACOIAS_003783"
     CDS             381..1112
                     /locus_tag="ACOIAS_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703428.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(1269..1475)
                     /locus_tag="ACOIAS_003784"
     CDS             complement(1269..1475)
                     /locus_tag="ACOIAS_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703429.1"
                     /translation="MSKYSSLWEYFKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          683 a          168 c          169 g          590 t
ORIGIN      
        1 taaaaatttc cagactctag cccctttttg aatattttaa aatttaactt aaactaagca
       61 aattctataa tagcttatct ctaaatattt aaatttaatt tatcatacaa tattacttat
      121 tataaaactt aaaacatata aaaatatatt ttaaagcaat aattttattt tacttattaa
      181 atatttggag ttattaaaat cttttctatc taaaaaatat tgatattaaa tatctaataa
      241 gaaaatttaa taagaaaaat atagattata tcaaaaaaat attctattat aatctttaga
      301 acaaacagta gtaaatgtta tcttcataaa caaatttatt tttcacaagt acaatactta
      361 tttaaaaaag gaggtttaat atgttaagta aaaaaaatgt aagtttaatt attttagtaa
      421 taattttaag tttattaact gctatattta ttttcaataa taaaaatact aacaataaat
      481 tgaaagaagt tactaagaat aatgaagaaa tagtacctaa aaacttagaa ggtttaagta
      541 tagaacaatt aatagagatt agagataaag ccattgataa aaatttagaa ggtcttaggg
      601 aatttggaga agattcagta aaaaaacgta aagattcaat tttaaacttt gaatttggat
      661 tagtagcttt tgttaacaag aattatagca acttaacaga aaaagagaaa ttaataagac
      721 aaattaatat tgttaactat gaaaactcta taaatgatat aatggatcct tcaggttcat
      781 tctttgaatt agcaaatcag aattaccaag tgaatgagtt tctattaaaa aacggaaaag
      841 attataaaga atggtcatat gaaggtctag attttctaat tgatcatcta gaagaactgt
      901 gcaaatttgt tgattttgat gtaaaagatg taatagatat tattgatcct acaataaaaa
      961 cagacctttc agaggaacaa aagaaatctt tgaatgataa attaaagaaa atatcttcaa
     1021 gtgatacttt aagtgaagaa gttaaaaagg agataaaaaa ctgggaaaac aatttaaata
     1081 atttaaatat gaataaaaat aattctaatt aattttctca taagaaaaat gcttatatcg
     1141 tgataatgtg aaatgtacat aattataaac aaaaaagggg ctgtgacact aaaaaatagt
     1201 gctacgccct tttttatttg taataatcat caatacaaat tattttatta tatgtaatta
     1261 tttatgtctt aaccaatttt atcgaaaata acagtcttct cttttaaaga gatttttcct
     1321 acttgatatc catattcatt taattccttt ttatatttca aaaacgaatg gtctatctct
     1381 atcccagcaa tatcttttat ttcttcaaaa gttaatttaa acgattgact attgtttttc
     1441 ttaaaatatt cccataaaga actatattta ctcatcttat atcactcctt ttaaaatcat
     1501 atcttatcta ttaatttata ttctatcatt attaatgtat aaagtcatgc tacttattat
     1561 ttatatataa aaatatccaa ataataagta gcaatttttt atagtcataa
//
LOCUS       DBGJFU010000257         1572 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.262, whole genome shotgun sequence.
ACCESSION   DBGJFU010000257 DBGJFU010000000
VERSION     DBGJFU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.262"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(81..1373)
                     /gene="brnQ"
                     /locus_tag="ACOIAS_003785"
     CDS             complement(81..1373)
                     /gene="brnQ"
                     /locus_tag="ACOIAS_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGZ2703430.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIISPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          532 a          284 c          201 g          555 t
ORIGIN      
        1 ttaataattc ttttaaatat aaaaaggaat tgcctatata atttaaaggc aattccataa
       61 ttatcaatta gtttttatta ttatgcagtt ttactgcttt tagatggaat aaagtttcct
      121 attattcctg ctattacaac tggaactatc cagttaaatc ctaatgaagc aaatggtaag
      181 ctattaacta gaggaatttt tacaccaaaa cttgttgcaa ctgttaatac acttactaat
      241 aatgccatgt atgcagcaca tttgaacacg ttgtcatttt taattttatt actaaataat
      301 gctagtataa ctagagttat agttgctgga tataccatac ttagtatagg agaagagaat
      361 tgtattattg tacttactcc aaagtttgat atgattgcac taaatacaca aactactata
      421 actatatgct catactttaa ttttccatct gtaagtgttg agaaatattg accagctgct
      481 gaagaaagac ctatagctgt tgttaaacat gctaatgcaa ctataatagc caataaaatt
      541 ttacctgtag ttccaagtaa agctgctgtt attgcaacta ttaaagctgt ttgttgaaca
      601 tcttgtccat attgttgaga cacagttgct ccaagataac ataatccacc ataaactaat
      661 gccattccta ttactgcaat tacacctgca cttattgtgg ctttcatctt ttctttctca
      721 cctgtataac ctttagctat tactgacatg attataattg atgcaaatac actagctgcc
      781 aatgcatcca ttgtttgata accttgagtt ataccttctg caaatacatt ttgtattaaa
      841 gaactgtctc ttatttctcc taatggagat atgatacctt taactattaa tactgctaaa
      901 gctataagta aagctggagt taatattgac ccaactatat ctacaacttt tgatggtctt
      961 attgttaaaa ccaatactag agcaaagaat actatagaga atattactgg gcttattcca
     1021 gaacctattg taggtaatat acccatttca taagttgttg ctgctgttct aggtattgct
     1081 aaaagtggcc ctatacagat taccattgca catcctaatg caatacttaa tcctttgcct
     1141 gctcttgaga acatcttcga tgtgtctcca ttgaacttag ttgctgctat aactgctaat
     1201 aatgccaaac caccatctgc aaaaaggaat cctatgaaag ctattagcca actacttcca
     1261 gataaaactc ctagatatgg agggaagatt aagttccctg caccaaaaaa cattgcaaat
     1321 agtgcaaatc caattacaat tacatcttta aaattgattt gtttctcacc catttaaagc
     1381 acccccattt gaattttact accttttact aaaaaataag tccaatctct tcattaaatg
     1441 aaaacctttt gacgagattt tatttaatta tacatttttg acttaaattt gtcaattatg
     1501 tttcataatt tataaaatct tcacaatatt taatacaact ttcttaatta aacaaaatca
     1561 acctgtatac tt
//
LOCUS       DBGJFU010000258         1526 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.263, whole genome shotgun sequence.
ACCESSION   DBGJFU010000258 DBGJFU010000000
VERSION     DBGJFU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.263"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            346..>1526
                     /locus_tag="ACOIAS_003786"
     CDS             346..>1526
                     /locus_tag="ACOIAS_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGZ2703431.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     Y"
BASE COUNT          589 a          182 c          208 g          547 t
ORIGIN      
        1 taagtaaatc ttttatcgat attattaaga atatatatta catcaagttt attacgtgca
       61 aaccactatc aacttaaata cctaaggtaa cacttgaagt agtagattct tggtaacaat
      121 aattattgct atcattatct ttttttaatt aagtatttac tatttatata tcaatctact
      181 tttcactttt aatttaattc taacattttt ttatatattt tatgatatat aatgtgatat
      241 attctaatta atgatatttt caagtaaaaa aatgttactc tattataagg tgatagtata
      301 cttgaaaata aataaaacac ttaactttat ttaaggagat ttatgatgag taacaaacaa
      361 ggttctaaca tcaaagatgt aaaaattaaa aataagttaa taatattgaa attactttct
      421 acaaatacac ctatgtccag agttgattta gctaagtcta caggacttac taaaatgact
      481 ttatcaaatc tagttactga acttattaat gaaaatatga ttagggaaat agagtcatca
      541 actttaaatg cacattcatc aggtcgtaaa cctattttat tagatatttc taatatatcg
      601 ccttgtattt gtggaatcct aataagaaga aatatatgta aggtaattat ttcagattta
      661 aaaggtaata ttgtaaaaca actttctgaa acttacccca aatgtctatc aaatgatgtt
      721 ttaaaagata taataaaaag aatgtttact aaaataatga aaatcaacaa gagagatata
      781 attggaattg gtatttcttc aataggtcca gtagatgata taaatggtac catactaaat
      841 cccccagatt ttggaaatgt atcaaaccta aatatagtag attttataaa agaaatgtct
      901 gatttgccaa tatttttgat aaatgatgct aattcgggtg cattagcaga aaaaatgtat
      961 ggtttaggta aaaatatatc aaattatata tacctacaca taatgaatgg tattggagct
     1021 ggtctagttc tagaaaataa acttcatact ggcaacctag gacaaagtgg agaaatagga
     1081 catacatcaa ttaattttag tggaccattg tgcagttgtg gtaataatgg atgtttagat
     1141 tattatacaa gtgtcagtaa tctaattaaa agaattgaat cattgagcca tatttatcct
     1201 aactccccat taattaactg taaagatttt tctttagtca agttaataga tgaggcaaat
     1261 aataaagata gtcttgccat gtttttgctt gatgaatttt gtacatacgt atcatatgca
     1321 ttagttaata cacttacatt gattgattgt agttctataa taataggcta tgactttaat
     1381 atacctggta catttataga aaatacatta ttaaaaaaat tgactgcttc agcaagtttt
     1441 tctaaatata aaaaaataag tgtaaggcat tcaaattttg gagcaaatgc accattaatt
     1501 ggctctatag ccattgtaag ttataa
//
LOCUS       DBGJFU010000259         1512 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.264, whole genome shotgun sequence.
ACCESSION   DBGJFU010000259 DBGJFU010000000
VERSION     DBGJFU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.264"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            486..1193
                     /locus_tag="ACOIAS_003787"
     CDS             486..1193
                     /locus_tag="ACOIAS_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HGZ2703432.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          632 a          138 c          268 g          474 t
ORIGIN      
        1 aaaacttatt aagtattaga aaatgacttc ttattagagt aagtatctaa aatgatataa
       61 aaattatgat tactcattaa agtgactatt atagagtgga taagattact cattaaagcg
      121 attactgtgg agtagataaa attactcact aaaataacta tcatagagtg gataagatta
      181 ctcattaaag cgattactgt agagtagata aaattactca ctaaaataat tatcatagag
      241 tggataagat tactcattaa agcgattgct gtggagtaga taaaattact cactaaaata
      301 actatcacag tagataagat tattcattaa agttactatc acagagtgga taaaattaca
      361 cattaaaatt gtctgttaaa gaatgaataa aaaagagatt gctcaatata agaagcatat
      421 aaaaaataaa aaatcaaacg tataaaattt aataaataat aactgatagg gaaaggaaag
      481 atagaatgat atttacaaaa agtaataaat acgataagga ctttctcatg aaaaatatga
      541 tgggacctaa ttgtataaaa atattggaag aattaacaag taagataaaa ctagaaaagg
      601 gaatgcgtat tttagactta ggttgtggta aaggtatatc ttcgatattt ttagcaaagg
      661 aatttgatgc tactgtattt gcaacagatt tatggataga accaactgaa aactataaaa
      721 gatttaaaga gtttaaattg gatgataaaa tatttccaat acaggcagag gcacatgaac
      781 taccttatgc agaaggtttc tttgatgcgg tgataagtat cgattcttat cactattttg
      841 gaaacaaaga aggattttta gacaatcata tatcacctct tgtaaaagag gggggaatac
      901 ttgctatggc aatgcctgga ttaaaagaag atttcgtgga ttgtatacca gatgaactca
      961 ttccattttg gcaagataat atgaacttcc attcaattac atggtggaat aaactttggt
     1021 ctgaatcgga gtctgtgatt gtagagaaat gtgaagcttt aaattgtcat gatgaagctt
     1081 ggaaggattg gataaattgt gataattcat atgctataaa tgacaaaaaa atgatggaag
     1141 tagagaatgg aaagtatttt aatacaattt ctttgatagc gagaactaaa taattgtgat
     1201 gaatttataa aagtataaga taagtagaaa tataagttga atgagagtat aagaagcata
     1261 gaaatataag ttgaattaaa gtagaaggta aataaaaatg taagttaaat aaagagttta
     1321 tacaaaaatg attttttaga ttatttatac ccaatcgagt tgcttaggtc agttgggtat
     1381 ttttattttt taaagttaaa ttgcaaatac tattgtgtga tatatatttt aatactaaat
     1441 gtttgtttta taagaaataa actaatagaa aaaatagttt taacaatgaa agcaaaaaag
     1501 gtattgatga ag
//
LOCUS       DBGJFU010000260         1506 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.265, whole genome shotgun sequence.
ACCESSION   DBGJFU010000260 DBGJFU010000000
VERSION     DBGJFU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.265"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            191..436
                     /gene="cdd1"
                     /locus_tag="ACOIAS_003788"
     CDS             191..436
                     /gene="cdd1"
                     /locus_tag="ACOIAS_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Cdd1"
                     /protein_id="HGZ2703433.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLI
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(654..923)
                     /locus_tag="ACOIAS_003789"
     CDS             complement(654..923)
                     /locus_tag="ACOIAS_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HGZ2703434.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT          608 a          216 c          171 g          511 t
ORIGIN      
        1 ttatttgtat aaaactatat tatgatgtaa ttaagtgact cagtgattta gaggtggtaa
       61 aatatatata tatgaaagag cataaataaa ttgatataat aaaaaattaa atattaaata
      121 ttaactataa taaaaaaata tctggtaata ggtataatat aaaaagaata taccagaaag
      181 aaggtgaagt atgggagaac tacaaaaaat tccaggggta gggaaagcaa cagaaaaatc
      241 tttaatcatg ttaggatata caactattaa atcacttaaa gatgcaaacc cagcacaaat
      301 gtatgaaaag gaatgtttaa tacgtggaca gcacatagac agatgtcagt tatatgtata
      361 cagatgtgca gtatattttg catcaactga aaatccagaa ccagaaaaat taaagtggtg
      421 gtattggaaa gattaaaaaa tcaaaaaagt tagacataaa aaagagttga atatccgttt
      481 taaataataa aaactaatac tcaactcttt tttagtcgta aattttatgt aaatctattt
      541 gtatggctaa atatacccat caataaatgt ataaatatac caatcaacaa atgactaaac
      601 atactaccta acaaaggatt aaataaatta cttaacaaag cattctaaaa caattaatta
      661 gaaactgttt cttcaagcca ttttctacat tgctcaatat ttaccatagg gtcaaaacct
      721 tggtcttttc ccttatttaa aagcatatca caagggaaag tatcacattc accacaatga
      781 ttaagttcct tactttcaca gcaagtttta accttacatt ctccacccca aaatggtttt
      841 tccatagttg ggcaaccagt gcaatttact tgttcttttt ttgtacaact gtcacaacag
      901 acaccacatc ttgattcaaa catgttcaaa catcctttcc attatttaaa aatattataa
      961 aatgatttta gatgaataca aaagtgcaaa tagttgattt taggtcatct aaatgttaaa
     1021 cataaaatta ttagtactgg taaatttata aaaactattc caaaaatctt tttgaatatt
     1081 tcaaaacaca ataaattact tcaaatccct ttgaatatat tttacagcat tatagattgc
     1141 tttaaatctc tttgaatatt ctacaagact atagattagt ttaaatccct ttgaatatat
     1201 tttacaacat tatagattgt tttaaatctc tttgaatatt ctacaagact atagattagt
     1261 ttaaatccct ttgaatattt caaaacacca tagattactt taaatccctt tgaatattct
     1321 ataacactat aaattacttt taacttttat ataaccataa agtttttatc tggcacttta
     1381 aatggagttt tatatcaaat ttgccttcca tcgaataacc aaacttaatt taattacaca
     1441 ttccaagagt caaatattca ttcaatatat ttgataattc tatgttatag ctataattaa
     1501 atatag
//
LOCUS       DBGJFU010000261         1400 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.266, whole genome shotgun sequence.
ACCESSION   DBGJFU010000261 DBGJFU010000000
VERSION     DBGJFU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.266"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            228..1235
                     /locus_tag="ACOIAS_003790"
     CDS             228..1235
                     /locus_tag="ACOIAS_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGZ2703435.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNF
                     KIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSF
                     LQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIANLIYGIGLSIFLG
                     YVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEY
                     K"
BASE COUNT          531 a          118 c          167 g          584 t
ORIGIN      
        1 taaaaatata ttgtaatatt aaaaaaataa tagtaaactg attgcataaa aatgattttt
       61 atataaattt taaacagaag aataaatttc taacgaaatt ggatatttct tctgtaaata
      121 catattcaaa aaaagaatta taagagttta gtcagcagtc gtgtaagtaa taaggacttt
      181 ataaatatat agaaatataa tgatatgagt ataagtagaa aaattttatg atttttacaa
      241 ttatttttta taaaaaaggg aggttatgtt tgttgaattt acaatcaaaa tgggtaaact
      301 ttttaaagcc aacatatgtc agtgatgaaa taaaaagaaa aaaactacaa gttataatat
      361 ttttttctat aacttatggt ttatcttatt cactaggttt attagcaata tgttttaatc
      421 atttaataga ttcagaaatt ttagctcaat ttatgatgat attgccatta tcttcagttt
      481 ctattgcaaa attttacaca gagggaagaa ataatgataa atataatttc tattcatcag
      541 taatattatt ttttattatt tactttttta tatttattat tgaatcatta aagttaataa
      601 gtattaatca atttcaatta atgtatctta tgttaatttt aataagtagt ttatgtgtta
      661 ttgtttattc ttataaaata acaagtttat atatttttaa aaattttaag ataggtattt
      721 tgctattttt ttactttttg ttttctcaaa ttatttttgg attaataata agtgataatc
      781 aatttgatta taagaatttc ttgaattata ttcttcttcc tataatatca ttagtataca
      841 tttatccttt tttatgtgaa gagtatggtt ggagatcttt tttgcaaagt attttatttg
      901 atagatttgg caaaaaaata gggattgtaa tggtaggcac ttgttggagt ttatggcact
      961 tacctttaca atttacacta tatagtcctc aaactccaat aattggagct atagccaatt
     1021 tgatatatgg tattggactt tctatatttt taggttatgt ttatatgaaa actaagaaca
     1081 tatggttttg ctctataatg catgttctaa ttaatagctt aggattaatt tttaatgatt
     1141 ctgaaataat tattaattac catagtatta tacaaagatt gatatttata ctattatttt
     1201 atactccttt tctatttaca aaagaatata agtaagaatt acaattaatt tatataaaat
     1261 aaatttttaa gtaaatattt ttatagagag attgttatat tacatatcag aaaaatatta
     1321 aactaactaa atcaataaaa aagttggtgg ataacaattt ataattgtta gcacaacatc
     1381 atcttatgta ttgaaatcta
//
LOCUS       DBGJFU010000262         1369 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.267, whole genome shotgun sequence.
ACCESSION   DBGJFU010000262 DBGJFU010000000
VERSION     DBGJFU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.267"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(257..505)
                     /locus_tag="ACOIAS_003791"
     CDS             complement(257..505)
                     /locus_tag="ACOIAS_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2703436.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(536..997)
                     /locus_tag="ACOIAS_003792"
     CDS             complement(536..997)
                     /locus_tag="ACOIAS_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2703437.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(1035..>1369)
                     /locus_tag="ACOIAS_003793"
     CDS             complement(1035..>1369)
                     /locus_tag="ACOIAS_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2703438.1"
                     /translation="FILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMK
                     SFSRLVRINKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSD
                     FYSEIAKF"
BASE COUNT          473 a          202 c          151 g          543 t
ORIGIN      
        1 attctaaaaa atataataat tttatttgtt gttttaatat tataacacat ttgataatgt
       61 ccaattcata tatgcaaaat ttatatactt taaaatatct aagaaattat tcaataaaaa
      121 aatactattt tttaaaaatt atcattattg ataaagctct actatttata ttttttagtg
      181 tattcatgta taataagtaa aagcacaacg gtatatactg ttgtgctttt ttgtaattaa
      241 taaacaatat tttggtctat aacaatgaaa aatggctact ttccataatt gatttcatct
      301 gacataagca aaaagggtgt ccagctgggt caatcataac tctccagtca tcagtaaatt
      361 gctcctctgc gattgtagct ccacaatgaa ttgcatattg aactgcttct tctaaatcat
      421 taacagcgaa atccatatga gccatttgct gttgagattc aggtttttct ggttctatta
      481 tgccccaagc ctttgttttt tgcaatataa agaaaaaatt atatatttta atattttata
      541 attgttgaaa ttgtgtaata aattcaactg gatacatagt gcgttttgaa atttcttcta
      601 aagctaaacc ttgttttaaa agcctcttta tgacgctttc catactttct gaaatttcta
      661 taatattttt atcaaaatca taaaacctaa aagttctttg accccatggg tattctttta
      721 tataatgcaa aaattcaata tttcctattc tatttatctt actttcccat tgtttgatgt
      781 cattaacctc aaaatacaac tgaaagttat ttgattgttg atatattggt aaatcatatc
      841 caattatagc tttataatta ctttgtaatc caaatccatt ttcaaatgat acatgttctc
      901 ctaaatctaa tgtgattttt tgctccataa catcttcata aaactttttt gatttattta
      961 tgtctttgac aattagcaat actcctccaa atttcatctt atcactatcc tttctttgtt
     1021 ataagtatta taacttaaaa ttttgcaatt tcactgtaaa aatcagacat attagctcgg
     1081 tatttttgag gagttattcc acagacaatt ttaaaatctt taataaaatg tggtatatca
     1141 tagaatccta attcattaca tatagacatc aatgaagttt tattattgtt tagaagtttt
     1201 atagatttat taatacgtac caaacgagaa aatgacttca tactcaaccc taggtactga
     1261 ttaaagagtc tgtttaaatg cctagaacta taaaatactt ttttagaaat ttcttgtgaa
     1321 gatatatttc cagagttttg aataatcaac tgaattgcca aaataaact
//
LOCUS       DBGJFU010000263         1363 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.268, whole genome shotgun sequence.
ACCESSION   DBGJFU010000263 DBGJFU010000000
VERSION     DBGJFU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.268"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            28..495
                     /locus_tag="ACOIAS_003794"
     CDS             28..495
                     /locus_tag="ACOIAS_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HGZ2703439.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            506..721
                     /locus_tag="ACOIAS_003795"
     CDS             506..721
                     /locus_tag="ACOIAS_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2703440.1"
                     /translation="MAIVVNLDVMMAKRKISLKELAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          507 a          148 c          168 g          540 t
ORIGIN      
        1 tactttatat aaaggagatg atattttatg attacaaaat tttttagtaa tatattatca
       61 gtatttctat attttctatt atcactttta ggagttgctg gtataatatt cttatgtatg
      121 atacctatgt ttttatatga ttttagttct gacaaagtaa ttgctttaat tgcaagaata
      181 ctatattttt ctgactactt tatagtcatc ttattcttga tatttataat taatagtact
      241 aagtattccc cattcgtact tgaaaatgta aaaaggttta aagtaatggg attttgttta
      301 ttgataaaca ctataattga atgtatcaat ggttataatt taaatactaa taatataaga
      361 atttttggaa ctgatagtgg cggtatctca ccacttatgg ttatctcttt tatatttgca
      421 ctaatgtgct ttgtaattgc agaaacattt gataaagcta taaaaataaa agaagataat
      481 gatttaacga tatagaggtg atgttatggc aatagtagtt aacctagatg taatgatggc
      541 aaaaagaaaa atatcactta aagaattggc agaaaaaata gatattacaa atgcaaattt
      601 atcaatttta aaaactggaa aagcaaaagc tattagattt accactctaa acgaaatttg
      661 caaagcttta gactgtcagc caggtgatat acttgaatat gtagaagatg aaaatgatta
      721 aaaaagagtg tttagaaaca ctctttttta ttatactata tgatttttat tcttttacta
      781 tattattgac attaatatta actttgttta aagtcataac aaaatatgtt ctaagaaaga
      841 aactgttcct ttatctattt gttatccatt acctataaga actgtatcac actaaataat
      901 acttttaaat tgataatttt accacagatt cctatataca ttcttttcta cataatattt
      961 atttaaactg ttagcaaaac atggaacttt ttcatttact tatacctcca tttatattat
     1021 tgcatagctt atagctatgt gttataaatt acattattga tgattttatc taacatttgt
     1081 aatcttttag atactctttg aagttacaac actgttatac tctaattaag taaactaaat
     1141 ctgatacaag ttggtgatta tatgaaaaga aaactttcaa cctattttaa aggatatatt
     1201 ttaggtttat gtatagcatt tttattattt acttttatat taaaaaaatt aattatagaa
     1261 aatatcattg gaataataat tggaactgga attgcatttt tactgattta cctatttaat
     1321 aaaaaaagaa aataatattc tataaaaagg tcttttagtg aat
//
LOCUS       DBGJFU010000264         1349 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.269, whole genome shotgun sequence.
ACCESSION   DBGJFU010000264 DBGJFU010000000
VERSION     DBGJFU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.269"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..>1349
                     /locus_tag="ACOIAS_003796"
     rRNA            <1..>1349
                     /locus_tag="ACOIAS_003796"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          357 a          292 c          408 g          292 t
ORIGIN      
        1 caaggtttcc aggggaaggt tcgtccgccc tgggttagtc gggacctaag ctgaggccga
       61 aaggcgtagg tgatggacaa caggttgata ttcctgtact accgataacc gtttgagaga
      121 agggatgaca cagtaggata agctaagcac actgttggtt atgtgtgccc aagcattgag
      181 gcagtcaaag taggcaaatc cgctttgata atgctgggat gtgatgggga gcgaaattta
      241 gtagcgaagt agctgatttc acactgtcaa gaaaagtctc tatcgaggtt aaaggtaccc
      301 gtaccgcaaa ccgacacagg tgggtgagga gagtatccta aggccagcga gagaactgtt
      361 gttaaggaac tcggcaaaat gaccccgtaa cttagggata aggggtgcca ccatcaggtg
      421 gccgcagaga ataggcccaa gcgactgttt accaaaaaca taggtttctg ctaagtcgca
      481 agacgatgta taggagctga cgcctgcccg gtgctggaag gttaagggga tctgttagag
      541 caatcgaagc agtgaactta agccccagta aacggcggcc gtaactataa cggtcctaag
      601 gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaac gatttgggca
      661 ctgtctcaac aacagactcg gtgaaattgt aattccggtg aagatgccgg atacctgcga
      721 caggacggaa agaccccatg gagctttact gtagcttgac attgggtctt ggtactacat
      781 gtacaggata ggtgggaggc tttgaaacca ggacgccagt tttggcggag ccatccttgg
      841 gataccaccc ttgtagtact gggactctaa ccataggcca tgaatctggt cttgggacac
      901 tgtcaggtgg gcagtttgac tggggcggtc gcctcccaaa aggtaacgga ggcgctcaaa
      961 ggttctctca gtacggtcgg aaatcgtacg tagagtgtaa aggcaaaaga gagcttgatt
     1021 gcaagacata caggtcgagc aaggatgaaa atcggactta gtgatccggt ggttctgcgt
     1081 ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt atctccccca
     1141 agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcatcaca tcctggggct
     1201 gtagtaggtc ccaagggttg ggctgttcgc ccattaaagt ggtacgcgag ctgggttcag
     1261 aacgtcgtga gacagttcgg tccctatccg tcgcaggcgt aggaaatttg agaagacctg
     1321 tccttagtac gagaggaccg ggatggacg
//
LOCUS       DBGJFU010000265         1337 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.270, whole genome shotgun sequence.
ACCESSION   DBGJFU010000265 DBGJFU010000000
VERSION     DBGJFU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.270"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            27..101
                     /locus_tag="ACOIAS_003797"
     tRNA            27..101
                     /locus_tag="ACOIAS_003797"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="ACOIAS_003798"
     tRNA            116..200
                     /locus_tag="ACOIAS_003798"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="ACOIAS_003799"
     tRNA            210..293
                     /locus_tag="ACOIAS_003799"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="ACOIAS_003800"
     tRNA            323..399
                     /locus_tag="ACOIAS_003800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="ACOIAS_003801"
     tRNA            407..482
                     /locus_tag="ACOIAS_003801"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="ACOIAS_003802"
     tRNA            571..659
                     /locus_tag="ACOIAS_003802"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="ACOIAS_003803"
     tRNA            663..738
                     /locus_tag="ACOIAS_003803"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="ACOIAS_003804"
     tRNA            745..818
                     /locus_tag="ACOIAS_003804"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="ACOIAS_003805"
     tRNA            833..909
                     /locus_tag="ACOIAS_003805"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="ACOIAS_003806"
     tRNA            933..1009
                     /locus_tag="ACOIAS_003806"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="ACOIAS_003807"
     tRNA            1017..1093
                     /locus_tag="ACOIAS_003807"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="ACOIAS_003808"
     tRNA            1102..1177
                     /locus_tag="ACOIAS_003808"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="ACOIAS_003809"
     tRNA            1185..1258
                     /locus_tag="ACOIAS_003809"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="ACOIAS_003810"
     tRNA            1265..1336
                     /locus_tag="ACOIAS_003810"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          320 a          309 c          355 g          353 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatatatatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DBGJFU010000266         1334 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.271, whole genome shotgun sequence.
ACCESSION   DBGJFU010000266 DBGJFU010000000
VERSION     DBGJFU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.271"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            22..1302
                     /locus_tag="ACOIAS_003811"
     CDS             22..1302
                     /locus_tag="ACOIAS_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGZ2703441.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          571 a          172 c          159 g          432 t
ORIGIN      
        1 taaagataaa aggagtattt tatgaacata cttggattaa tagttgaata taacccattt
       61 cataatggac acatatatca tttatctaaa tctatagaaa aaacaaatgc aacacatact
      121 gtagcaataa tgagtggaaa ttttctacaa agaggcgaac cagctttatt tgataaatac
      181 actagagcaa aaattgctgt cgaaaatggt gtagacttag ttatagaatt accaacttta
      241 tttgcttgtc aaagtgctga aattttttct catggtgcta taacaatttt aaattcacta
      301 aactgcataa attctatatg ttttggtagt gaagaaggaa atacagaaat tctttataat
      361 atagctgaaa ttttagcaaa tgaaccaaat gaatttaagt tacttttaaa aaaatattta
      421 gatgatggtc tgttatttcc aacagctaga gctaatgcac tttttgatta tatacatcaa
      481 gacaaaaaaa ataattttat acaaaattat tcaaaggaac aattatcaaa aacattaaat
      541 tcatcaaaca acatactggg tatagaatac ataaaaaacc tcatccattt aaacagtagt
      601 ataaaaccat ttactatctc cagggtccaa tcagagtata attccgaaga aattgagagt
      661 gatatttgtt ctgctactgc tataagaact tcacttaaag aaaatacaga cctttttcat
      721 ttaaaaaatg ttattccaga aaaaacttat aacatcctaa atgaaaaaat agataatggt
      781 tttttaccta tgtttgatga cttcttcttt gatattttaa aaacaattat acttagagat
      841 atggacactt taaaagagta ttttgaagta aatgaaggca tagaaaacag aatctataaa
      901 gatatcttta catctgaatt tctacatgaa ctacaattag caattaaatc caagcgatac
      961 actttaacca aaatcaaacg aactctaaac aatatattgt taggtattaa aaaaagcgac
     1021 atgcaactta taaaaaacat agatactata ccttatataa gaatacttgc ttttaatgat
     1081 aaaggtagag aaattctaaa agagataaaa aataaatctg aaataaaaat aataaacaag
     1141 ttttcaaata tatcattttc attagatgat acgattttta aaacactcat aagttatgat
     1201 ataaaagcta caaatatata taattcaata tattataaaa acaataaaga tttagtaaaa
     1261 ggccctatgg atttttatac tcatccaata tatgtaaagt agttttttaa aataactata
     1321 tttataaaaa tcat
//
LOCUS       DBGJFU010000267         1260 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.272, whole genome shotgun sequence.
ACCESSION   DBGJFU010000267 DBGJFU010000000
VERSION     DBGJFU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.272"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(88..1128)
                     /locus_tag="ACOIAS_003812"
     CDS             complement(88..1128)
                     /locus_tag="ACOIAS_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HGZ2703442.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          430 a          198 c          145 g          487 t
ORIGIN      
        1 gtctatagaa aaaccaattt ttgtataaaa ataaaaatta aagttaatta tttgttaaga
       61 attgactttg gtaaaatata taattaatta ttctttaagc atataattta taagtaattt
      121 tagtgtattt tcaatagctt ctatatgtgt tctttcataa tggtgactat tgtttgttcc
      181 aggaccaaca catgctattt gaacatcttc tccccaacga atagcttctg aagcatctga
      241 accatagaaa gtaaatacat cgacagcgta gtctaaatta ttgtcttctg caacatcaac
      301 aagcttgttt cttaaattaa aatcatataa tgataaatta tcttttgcaa ctatagttac
      361 accatgttca ctagatgttt gacttggtcc agtaggtgct atatctattg ctacaaattc
      421 ttttgttttt tcaggtaaac atttagaaac accatgtcca atttcttcat aattacttat
      481 ataaaagtta gtagtatgct taggtgttaa attgttttct ttaaaatatc tacatatttc
      541 aagaaccatt gctacagctg atttattatc tatatatcta gattttataa aaccagattc
      601 tgtagcctca aatctatggt ctaggcatac aaaatcccca acacttattc ctaaatctaa
      661 tacatcttgc tttgtaaata ctttttcatc aagtctaata atcattgttt cttcatttct
      721 ttcagtggca gcttttgttt gaccatatat atgagtagaa gcatacttaa atacaacact
      781 tcctctgatt tttttacctt ttctagttat aacagtacag ttttctccct ctatagctgc
      841 ccaacatcca ccaccaactt tatggtattt aagagtacca tcagatgata tttcttttac
      901 tatagctcca agtgtatcca tatgtgcaga tattgttatt tgatttgaat catcttcacc
      961 aggaagagta gctataagtg cacctttttt tgtaagaata gtgtcaagac ctaaattttc
     1021 aaaatctttt ttgatttcta aaactgcatt ttcagtatat ccagctgggc ttggaatgtc
     1081 caaatatttt ttcattaaat ttatagtatt agttaaattt aaattcatat tgtttcctcc
     1141 taaataaata tatttataca aattataaca taaatacaaa tgttctgaaa actaaaatta
     1201 aaagaataga atctaaacat atttaaaaaa aaagtatata aaataaattt gatttatatt
//
LOCUS       DBGJFU010000268         1241 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.273, whole genome shotgun sequence.
ACCESSION   DBGJFU010000268 DBGJFU010000000
VERSION     DBGJFU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.273"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(48..485)
                     /locus_tag="ACOIAS_003813"
     CDS             complement(48..485)
                     /locus_tag="ACOIAS_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGZ2703443.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(742..891)
                     /locus_tag="ACOIAS_003814"
     CDS             complement(742..891)
                     /locus_tag="ACOIAS_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HGZ2703444.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(904..1134)
                     /locus_tag="ACOIAS_003815"
     CDS             complement(904..1134)
                     /locus_tag="ACOIAS_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HGZ2703445.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
BASE COUNT          433 a          167 c          134 g          507 t
ORIGIN      
        1 caataaattt gcaattagtg atattaataa attactttag atttttatta aatgtttagc
       61 ttttctttaa tattttttat ataacgacga tttataataa ctttttcgtt gttttttaaa
      121 gtagcttgca tgcgattatg tgatattact tttatacttt ttatgtagtc catatttaaa
      181 atcatggatt tagatattcg tgagaaggat gtattagcta ataattttga taatttatat
      241 atttttaaat caatttcaaa tacatcatct tcataataag caaatgtttt atttccaact
      301 gtctcaaagt ataaaatatc acagacattg agtttataaa tctctcctga ttttttacct
      361 tttatatcaa gtaacggtgt actcaataat gaaattattt tttcaatgtt attatctaga
      421 gcagaacatt ttatggttat ttcaatatca tcacaattaa taccttgttc gacatgtatt
      481 ttcattaatc atcacctcat tttattactg tcattagaat agcattatat cttattgagt
      541 ccaactagat gcgttctaaa tttcaaatta agcatagtga aattcaaaaa taaaacagtg
      601 aaattcaaat ttgaggtggt aaaattcaaa aaaatactat ggatatttgg ataaattatc
      661 caaatatcct agtatttcac atagaatatg tctaattatt ttacaaatag acctatttta
      721 tgacttacat atattgtcaa attacttctt aaatatcaaa atatttttgg ataatgtatc
      781 aatactgttt gataaagtat ttaatttacc ctcaattcta accaataagt acatggatag
      841 agctatagga aaaccaacat tggttattaa cagttgtaaa ctatcatcca taaaattagc
      901 cccctattct ataactaagt cataaggagt ggtatttgtt tccacaacct tggcttcaac
      961 aacagaagct aggtcaaatc ctccaaccga aaaaatattt ttagatacta tcaaatccat
     1021 gacagatttt atattttctt cagttaaatc atcctttgga ttttctatag aaaaagaaac
     1081 ttgtttatcc tttacatttt taaagctcat gactaatttt ttcttaattt ccatataatt
     1141 acctccgata taataattta tattttaatt gtttgcttgt taatattggt attttaattg
     1201 tcaatattaa tgttttgctt gttagtatta atatttcaat t
//
LOCUS       DBGJFU010000269         1160 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.274, whole genome shotgun sequence.
ACCESSION   DBGJFU010000269 DBGJFU010000000
VERSION     DBGJFU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.274"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(137..907)
                     /locus_tag="ACOIAS_003816"
     CDS             complement(137..907)
                     /locus_tag="ACOIAS_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGZ2703446.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT          429 a          208 c          124 g          399 t
ORIGIN      
        1 aaactatatt caataaatat ataataaagt aaaaaaaaaa aaataacaac atataaaaga
       61 atataaataa tttaaaaaaa gcatcaatat cactgatatc aatgctttaa aaggcaattt
      121 aattcgtatt atttatctaa ctatttttga catagcaaag ataataaatc cctccaacta
      181 ctatggaacc tccaacgata tttccaagcg ttacaaatac caaattagtt ataaattgaa
      241 ccatagttat atttgctcct aatgccattc ccattggtat aaaaaacatg tttgcaacac
      301 tatgttggaa tccacataat acaaatagca ttattactgt ccaacatgaa gccaattttg
      361 acattatatc ttgagatgct gtagccatcc atacagctaa aacgactaga atattacaaa
      421 gtattgcttt taaaaacatt gatgctatag ttgaattaac cttagcttct gccacagcta
      481 tagcttttgt agctgcatct cctgtcaatg tagaagaata aaacacaagt gcaactagca
      541 aaattgaacc tactaaattt gcaaaataaa ccagtcccca gtttcttaac atttttccta
      601 atgttatttt tttgtccata agtgcaatag ccattaaggt gtttccagtg aataattctg
      661 ctcctgccac tactacaagc ataagaccta ctggaaagac catagcactt aaaaagctag
      721 ctacccctgg gtctatcttg ccaaaagttt gactaactat catactcccc aatgccccaa
      781 aacctataaa tgctcctcca aataaaccta atagaattgc gttttgtgtc gaaatatttg
      841 cttttttaat gcctgcactt atagttgctt gtgcagtttc cccaggtgtc aagaatcctt
      901 tactcataaa tccttccccc ttaaaaatta tactttccct tgtcttttta tggcaataat
      961 tatataataa caatatgata tataataaat tttattaaca taaaaaatat actatatact
     1021 tatatatact tgtctcatat aattatacta tttattatat aacaactttg ttaatttcga
     1081 aataatttat attgaatttg aaaaatttac cttttgatta tttattttac tttttaatta
     1141 ttgaggtatt atatataata
//
LOCUS       DBGJFU010000270         1155 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.275, whole genome shotgun sequence.
ACCESSION   DBGJFU010000270 DBGJFU010000000
VERSION     DBGJFU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.275"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            431..898
                     /locus_tag="ACOIAS_003817"
     CDS             431..898
                     /locus_tag="ACOIAS_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGZ2703447.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          447 a           78 c          186 g          444 t
ORIGIN      
        1 ataattagaa agattatgaa aactaaatta gaagaactaa aaactatatt tttattaatt
       61 agaatatttg aataatctct ttaatcatat aaaatacata tgcttgtaat atttaaaggt
      121 gatattaata ttattagtgt atattaatat tctcttgaaa ttaaaaaaac atagatattt
      181 cagtaatcta aaatagtcta caaaaataac agttttagca aaattaataa gaatacatat
      241 agatttaaac ctaaaagtat gaatgtgtct gttttttaca agattaaaat ttatattata
      301 tgatacaatt aacttaacta tttcaataag acttataatt tttttcttta tactagacct
      361 tattgaaacc tttatagttt aattctaatt atatattata aataaagtat gtgtatatta
      421 aggaggtaat atgaagtttt taaatgtttt aatagtagtt gaagatattg agaaatcaaa
      481 aaagttttat tatgatgttt tggggttaaa ggtaatctgt gattttggag aaaatgttgt
      541 attagaaggg aatatatcat tacaagaaaa aaagctttgg ttagagttta ttaataagag
      601 tgacagtgaa gttaagttca atggaaatga tgctgagctg tattttgagg aagattattt
      661 tgatactttt gttgaaagat tgagtacaat gagagatata gattatgttc atctagctat
      721 agagcatagg tggggtcaaa gagcaattag attttatgat ttagatgggc atattattga
      781 ggttggagaa actatgtcat ctgtgtgtag acgtttttta gatagtggtc tttcaataga
      841 tgaggtagct aaaagaatgg atgtaactgt cgaatatatt gaatcagttt tagaatagat
      901 ggattatatt aaagtaaaac tatattttgt atagaagtaa gggctgtaag taaacttgta
      961 tggaaacaaa tttacttaca gtttttgttt ttgtaagata cagaatataa tatttgttat
     1021 aattttattt ggatttttaa aaagtgcatg attttcctta catattataa tagaatttaa
     1081 tatttattgg atgatttaaa tttagaaaaa tataagtatt agcttataaa agataattat
     1141 acttactatc aagct
//
LOCUS       DBGJFU010000271         1122 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.276, whole genome shotgun sequence.
ACCESSION   DBGJFU010000271 DBGJFU010000000
VERSION     DBGJFU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.276"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(90..920)
                     /locus_tag="ACOIAS_003818"
     CDS             complement(90..920)
                     /locus_tag="ACOIAS_003818"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGZ2703448.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK
                     "
BASE COUNT          396 a          181 c           97 g          448 t
ORIGIN      
        1 aagtttccat ttttcaatat gtaagctttt attatgttat tttattgtta aaggtttcaa
       61 gtttttattt attcatttta aaaaaattat tatttttgta aaactaatcc catagtcata
      121 ctatcgtcag aatatgcttc acccttaaca ttactaaaaa actcaacaga cttaaatcca
      181 atattttcaa cttctaataa gaaactttct tttgaaaaat agtgattcca aacataaaat
      241 acattttcat ctttttcggt tattacaaga gtttgttcta atgtattata atcattgtat
      301 ttacaattat cttgtagaca catatatttt tcattactcc aaaatcctcc atcttcattt
      361 atttcccaat atttaatttc ctcaaaatta ttgtatttat ttattgtaaa tacatctaat
      421 attaattttc ctccaggttt taaactatca taaatttttt ccattaataa ccttctattt
      481 tcagttgaca aagctccata atcacaataa attaaagtag ccaaatcaaa ttcctcatta
      541 tagttcatat ttaaataact ttggaacaag taatttatgt ttaaattagt ctccttattt
      601 ctattttgtg catagttaat agaacgtttt gagaaatcaa ttccagttac cttataacct
      661 ttttgtgcaa atttctctgc atataaacct ggaccacaac ctaaatcaag taatttagga
      721 taattagctg gatttgcaac tgtaactatc caatttacag aatcctcgat aaagttaaaa
      781 tttctacttg ctccttcaaa attagtgtct aagtgtgctt ttaataattg ctttgaaata
      841 tactcatcat cccaaaaatt aatttcactt tgtttataaa gctctggtct ttctaaatac
      901 atacataatt tgtttaacaa aacataccac tccttaaaat ttatttttaa aataaaaaaa
      961 ccacagagtt tactctgtgg tttaacttca aaaagatgag tttaaataag agtaaaatag
     1021 aaccaaatat ttttaagcac aacaataaaa ctatagcttt actttgtttg cttattaaat
     1081 ttgattttat tcactctttt ccagcaatac ttatatcaac ga
//
LOCUS       DBGJFU010000272         1097 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.277, whole genome shotgun sequence.
ACCESSION   DBGJFU010000272 DBGJFU010000000
VERSION     DBGJFU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.277"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(224..934)
                     /locus_tag="ACOIAS_003819"
     CDS             complement(224..934)
                     /locus_tag="ACOIAS_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HGZ2703449.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT          396 a          146 c          121 g          434 t
ORIGIN      
        1 aaattaaatt ttgaaagatt gaaattctag aaactaagct ttaaaaaatt aagtttgaaa
       61 aaacaaaact ctaaaaacta agcttcaaaa aattaaattt tgaaagattg aaattctaga
      121 aattaatctt caaaaaatta aatttgaaaa aacaaaactc taaaaactaa gctttaaaaa
      181 gttaagtttc aaaaataaaa ttctagagat tgaacttcaa aaattaaggt tctaacatat
      241 accttgtaaa ttttcctaca acctcttgat attttttacg gcttatagga attttttcat
      301 tctgaacttt ggcataagtt tgagttatac tatctacatg gtttagattg acaatatacc
      361 tttgatgcgt acgtataaaa ttagagggaa gaacagtttc catatcattt aattttccgt
      421 agtattctaa tgttttatca aaggtaacta cagttaattt tcttagctca cttttaaagt
      481 agaggatatc ttttgtatta attttacaaa gattagttcc aatctgaact gtaataaagt
      541 tttctttcca ttgttctaat ttttttagtg cttcttttaa tactttacat attttttcag
      601 ttttataagg ttttaagatg tagttcaaag ctcctacatc atatccatga aatacatagt
      661 cagaaaaacc tgtgacaaag atgattatag tatcctttga atgttttcgt atagtttttg
      721 ctatgtcaac gccattactt tttccaagtt caatatctaa aaaaataata tctatgtaat
      781 aattttcgtg ttctagattg tatactagac tttctccatt tgaatattct tttatttggt
      841 aggggtagcc ccacaaatcc atttctcttg taatgatttt attcagcatg tttctttgtt
      901 gtttttcatc atcacaaata caaatcgaag tcaatttttt atctcctttc aaattttata
      961 tttatatagt taagtatata atatattttg ctacctagat atataaaatg aaataacaat
     1021 tatatccaga tagcaaaata aaatttttta tattagtata cttacactta aaactattct
     1081 attaaactta ttctgaa
//
LOCUS       DBGJFU010000273         1096 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.278, whole genome shotgun sequence.
ACCESSION   DBGJFU010000273 DBGJFU010000000
VERSION     DBGJFU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.278"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            141..281
                     /locus_tag="ACOIAS_003820"
     CDS             141..281
                     /locus_tag="ACOIAS_003820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703450.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(278..649)
                     /locus_tag="ACOIAS_003821"
     CDS             complement(278..649)
                     /locus_tag="ACOIAS_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGZ2703451.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          427 a          112 c          131 g          426 t
ORIGIN      
        1 taagataatt atctagggaa attttggata atcaaaatat attttataaa atcaaaatac
       61 aattacataa actttattaa aaatttgttg ggaatttctg actaacatta agaacgtaag
      121 tttgttgggg gatggtatat gtggaaaagt caaacactac tgttacaaaa aaagaaaaaa
      181 ttgaagatat aggaatgaaa ttagaacatt tattgagtga agatattact ttttatgaat
      241 tattacttga gattttgtat aaagaaaaag aagagaatta attctcttct ttaaggaatc
      301 tttttaaata ggcattatca atcatttttt ctatagcttc tctatcttct ttttcaagag
      361 aatatatttt ttctaagagc ttttttgttt tttcacttac tgataagttc tctatgatta
      421 atttatcttc tttgcttatt tttgtattat catttacgcc taataaataa tctgttgatg
      481 tttctaatgc ttttgctaat ctaactatta tttcactttt aggttctctt aaattatttt
      541 cataacgtga caagctagcc tcagttatac cgactttagt agcaagttct ttttgattta
      601 tatctaattc ttttcgtaga tttgctattc tatctcctaa actttccata tgtacatcac
      661 tccttaccga aattataagt tattttgata aaaagtaaaa taaaattacc aaaaatataa
      721 aaaaaaaagg ttaaaataat aaaatatatt gaaaattacc atttgtatat gtataatatg
      781 aattatacaa atggtaattt gatgagaggt ggtatgttga aataagaaac tgaattaatt
      841 aataaataca tagaaagttt tgaaaacctt taattgaaat ataaacaata tgtacaaaat
      901 tttttgcaag taaaattacc aaatgtataa aataaatcag tagcatagat aattttatgc
      961 tttaatacat tagattattt aatttagtta taatggtcaa aaatgcaatc tacaaataaa
     1021 acaaagtatt atcaaaaaag tattctttaa gaaaattttt tattagagat gtaattgata
     1081 taaataatcg tattaa
//
LOCUS       DBGJFU010000274         1043 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.279, whole genome shotgun sequence.
ACCESSION   DBGJFU010000274 DBGJFU010000000
VERSION     DBGJFU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.279"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            217..1029
                     /locus_tag="ACOIAS_003822"
     CDS             217..1029
                     /locus_tag="ACOIAS_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HGZ2703452.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
BASE COUNT          384 a          110 c          181 g          368 t
ORIGIN      
        1 tttatatgac taagttctat aactaaattg aaataatcac tttttcaaga atgttataat
       61 ttttttattg tattcttttt gctatagtct atgaataaat caatagaaaa tcttctaagt
      121 tgcttttaaa taatgacttt atggttcaat tgtactaaaa tagtatatgt aggttacaaa
      181 taatctaata aatattaaag tttggaggta atagatatgg cagattgtaa ttcatgtcca
      241 tcaaagggaa attgtaatag tcaatcaaat tgttctattg aaaataaccc taacaataaa
      301 tttggaaaaa taattggtgt tatgagtggg aagggtggag ttggaaaatc aactgtaact
      361 gctttacttg ctaataagct aaacaaaatg ggatataagg ttggtatatt agactcagat
      421 ataactggac caagtattcc tagacttatg ggtgtaaaaa atgtaaaagc ttattctgat
      481 ggctcttata tttaccctgt agaaaactca aataacataa aagtaatgtc tataaacctt
      541 atgatagatg atgaaaatga acctgttgtt tggagaggac ctttacttgg aggggttgta
      601 aagcaatttt atactgatgt actttgggaa gaattagact atttattaat agatatgcct
      661 cctggtactg gagatgttgc cttaacagtt atgcaatcta ttcctataag tggaatagtt
      721 atggtttcag tacctcaaga tttggtatct atgattgtat caaaagctgt taatatggct
      781 aaaaaaatga acataaatgt ccttggagtt attgaaaata tgagttatat ccaatgtcct
      841 gattgttcta aaaaaatcaa gttatttgaa ggagaatcta ctgaaaagtt tttagatgat
      901 ttagacttag agcttttagg agaacttcct atgactaaag aaattataga tattactcat
      961 aatggagtta cagaaattag tgatgattta gattcaatat tgactaatgt tgttgaaaaa
     1021 attaaataaa taaaaatttt ata
//
LOCUS       DBGJFU010000275         1037 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.280, whole genome shotgun sequence.
ACCESSION   DBGJFU010000275 DBGJFU010000000
VERSION     DBGJFU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.280"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            151..855
                     /gene="cwlD"
                     /locus_tag="ACOIAS_003823"
     CDS             151..855
                     /gene="cwlD"
                     /locus_tag="ACOIAS_003823"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HGZ2703453.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT          460 a          108 c          158 g          311 t
ORIGIN      
        1 ttaaataggg gcagaaatat aatatcatta cataataata tattaatacc aataaataaa
       61 gatatatcaa tattaaaaaa atttaaatgt ttgtactaat atatggaata ttatagtgtt
      121 tttataagta tacatataca gggtgatgtt gtgagaaagt acataaaaca tataattttt
      181 agttttgcaa tgatatgtct agtagtagta tccatatttg aaattaaaaa tatttctgaa
      241 gatgttatca agtatatgcc agtaacaaac aaaactataa ttttagatgc tggtcatgga
      301 ggcattgatc caggtgcatt aaataaggat aagagtacat ctgaaaagga tattaaccta
      361 gcaataacac ttaagcttag agagcttata gaatcaagtg gtggccttgt aatattaact
      421 cgagaagatg acagtagcct ttataaagaa gaaaataata aaactacaag acaaaaatat
      481 aatgagaatt tgaaaaatag aaaagaaata atatctaatt ctaatgccaa tatgtttgtc
      541 tctatacatc ttaatgcatt tgaacaatca aaatactatg gagctcaaac tttttatcct
      601 aaagacaaac aagacagtaa agaattatcg aagtgtattc aagaagaact taaaagagta
      661 gtagataaga caaacaacag agaagtcaaa ccaagggatg atatatatct tttgaaagaa
      721 aataatattc catcagtact gatagaatgt ggttttttat caaatgaaaa agagtgtaaa
      781 ctcttaactg atgaaacata tcaagaaaaa atagcatggg caatctacat aggaatacaa
      841 aaatatttaa gttgaaataa ctataaaaat aggtctaaat atgtaaatag cttgtattaa
      901 agcagttaaa atgcattaat ataggctatt tttattttga caaaaaaata tttaaaataa
      961 aagttaaaaa gttgttgact tagaataata tagatgatat tatatatgag tgcccaaaag
     1021 gagcaccaaa ataagac
//
LOCUS       DBGJFU010000276          952 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.281, whole genome shotgun sequence.
ACCESSION   DBGJFU010000276 DBGJFU010000000
VERSION     DBGJFU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.281"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..894
                     /locus_tag="ACOIAS_003824"
     CDS             <1..894
                     /locus_tag="ACOIAS_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HGZ2703454.1"
                     /translation="NNLIYAQNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPF
                     RLGDAIVKVNNAEVDGYSDFANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISF
                     NNLLSGFATLTYINPDTSEFGAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSV
                     GCINAKRENIIGSFTENTDFGIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNF
                     NKCKKYNIEIIDIENQKHPESKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGA
                     VSHAIENDPAVGYGVFIKWML"
BASE COUNT          404 a          106 c          147 g          295 t
ORIGIN      
        1 aataatttaa tttatgcaca aaatttacaa aaaaatgaac ctgaatattt aataccaatt
       61 ggaaacgtac ttcaaattga cgcagaatta aaaaatatca tagttagaaa tcctggagaa
      121 gcatctccat ttagattggg tgatgctata gtaaaggtaa ataatgctga agttgatgga
      181 tatagtgact ttgccaatac cttaaatgct ttaccagaag aaaaacaagt atctatttta
      241 ttaaatagag gtggacaact tataacaatg aaaactacca aacacatatt agaaaaaatt
      301 agttttaaca atttactttc tggatttgct actttaacgt atatcaatcc agatacatca
      361 gagtttggag ctgtaggtca tccaataagt gttggtaatg caagaaaaat acctattaaa
      421 acaggttcaa tttctacaac taagcacctt aatatagaaa aatcttataa aggtagtgtt
      481 ggatgtataa atgcaaaacg agaaaatatt attggtagct ttacagaaaa tactgatttt
      541 ggaataagag gtcaaattaa taactttgat atttccaagt taaaaaaata taaagtagca
      601 tctttagatg aagttaaatt aggaaaagca caaataattt tacaagataa ctttaataag
      661 tgtaaaaaat ataatattga aattatagat atagaaaacc aaaaacatcc agaaagtaaa
      721 agttttaaaa ttaaaattac agataaaaag cttttagctc aaactggtgg tattgtacaa
      781 ggtatgagtg gtactccaat agttcaagat aataaaataa tcggagcagt gtcacatgca
      841 attgaaaacg acccagctgt aggttatggt gtatttatta aatggatgtt gtaaataata
      901 taaataaata aataagtttt aagatgttat taagttatta ataatatatt tg
//
LOCUS       DBGJFU010000277          863 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.282, whole genome shotgun sequence.
ACCESSION   DBGJFU010000277 DBGJFU010000000
VERSION     DBGJFU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.282"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(107..592)
                     /locus_tag="ACOIAS_003825"
     CDS             complement(107..592)
                     /locus_tag="ACOIAS_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HGZ2703455.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(675..768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          287 a          131 c           68 g          377 t
ORIGIN      
        1 aatttattct ctttagttct gttataatgt ttctacaaaa aagaatccca ttaatgggat
       61 tcttttttgc gatttattaa tatatttaaa gcttgctagt atcgtattat aaacttaaat
      121 gctcttctat gtattgatta tatacatatc cagaatatcg tttttcattt ttattgatat
      181 taactcttgc ccattttcca acgctataac atacttcaac tttaaaacca tcagggattg
      241 tagttataac tttagattct aatgttctat catttctaag ttctaaactt aatccatttg
      301 ttcttatatt tcctatgtaa ttcttttttc tattttttat tgtatcatta ctattgtttt
      361 tagcattact taatctttct aaatatttat aaagtaacca acctttgtca taattactct
      421 caacttctgc ccatattcca tctacacact ttaacttaac aattttacct tctggtatac
      481 tttttaattt caaactattt atgtctggtt tttctcttaa attcaattta attttagtct
      541 taaaattgat gctagatttg cgaaaatttt ttaacattaa ttcagatatc atcttaagcc
      601 ctcctcttgc caaatattaa gttattaaaa tatataactc taaaaatact tttaacaaaa
      661 agaattatta tctttttggc aactggctga catgaccttt attacataaa gattttagtc
      721 cctatagttt tgcgtcatta gatttatcta atattgccta tatctgaata ataacataat
      781 ttgaacaatt aaaaaataat attacctcaa attttatatt atttaaatat aaattaaatt
      841 tttaattaat aataatcaaa aaa
//
LOCUS       DBGJFU010000278          769 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.283, whole genome shotgun sequence.
ACCESSION   DBGJFU010000278 DBGJFU010000000
VERSION     DBGJFU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.283"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            40..714
                     /locus_tag="ACOIAS_003826"
     CDS             40..714
                     /locus_tag="ACOIAS_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGZ2703456.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
BASE COUNT          277 a           96 c          141 g          255 t
ORIGIN      
        1 tagtatatat aatattatat agaagggggt gctagataaa tgaaccatca tatattgatt
       61 caatttgaat atatagcaag aatcattact gctggtttct gtggggcttt aattggatat
      121 gagagaaaga atagactaaa agaagctgga atacgcactc attttatagt ggcattagct
      181 tcagcactta taatcattgt ttccaagtat ggatttaatg atatagtggg tactccaggt
      241 attggtctag acccagcccg cgttgcagca caagttgtca gtggtatagg ttttttaggt
      301 gctggattaa tatttattag aaatcaatca gtaagtggtc ttactacagc tgctggtata
      361 tgggcaacag ctggtacagg tcttgccatt ggctctggat tatacttagt tggtattgtt
      421 tcaagtatca ttattgttat tgtacagata acacttcata gagataggaa atggatgaaa
      481 ctccctacag cagagcaagt ttctcttaaa atttctaaca gcactgattc catagaatat
      541 attgaaggta aatttaaaaa atctaatatt gaaattataa acatgaatat taaacgcctt
      601 gatggggatt ggttagaagt agttatatat actaagttac ctaaaggata taaaaaaaca
      661 aatttattaa aattatttag cgataatcct cttatagaaa ctttagaaat ataatataaa
      721 aattgaagct taaaaataat aatatattta atcaaggtca atatataaa
//
LOCUS       DBGJFU010000279          760 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.284, whole genome shotgun sequence.
ACCESSION   DBGJFU010000279 DBGJFU010000000
VERSION     DBGJFU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.284"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            172..270
                     /locus_tag="ACOIAS_003827"
                     /pseudo
     CDS             172..270
                     /locus_tag="ACOIAS_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            272..>760
                     /locus_tag="ACOIAS_003828"
     CDS             272..>760
                     /locus_tag="ACOIAS_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HGZ2703457.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKTGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNATISKEPSGR
                     YYV"
BASE COUNT          328 a           76 c          117 g          239 t
ORIGIN      
        1 ttacggttct tatatatgtt atttaaaaat aaatataaaa aaacttcaaa atacttgttt
       61 taagtaattg tatattttaa tattatgata ggttatgaaa aatggaagtt actcatggta
      121 gaggatatgc atatagtatg tcaaatatag acagccagta ttatggggag acatctttgg
      181 aatcctagtt atttcgttgc tactgtatca gaaaatacag aggagcagat aagaaaatat
      241 attaataatc agaaaaaaca agtaaagtga ggtggtagtg gtggaaaaag cttataagtt
      301 tagaatgtat ccaaataaaa aacaacaaga gttaattaac aaaacttttg gatgttgtag
      361 atttgtatat aataagtatc ttgctaaaag aatagatgtt tataaaaaca ataaagaaac
      421 ttttacttat aaacagtgta gttctgattt aaccaattta aaaaaagaat taaaatggct
      481 taaggaacct gacaaatttt cattacaaaa cgccttaaaa gatttagata atgcatataa
      541 aaaattcttt aaagaaaaaa caggatttcc taaatttaaa tcaaagaaaa ttaatagatt
      601 ttcatataaa actaacttta caaatggaaa cattatgtat tgtggtcaac atataaaact
      661 acctaaactt ggtatggtta agataaggga taaacaagta cctcaaggta gaatacttaa
      721 tgctactata tctaaagaac caagtggtag atattatgta
//
LOCUS       DBGJFU010000280          636 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.286, whole genome shotgun sequence.
ACCESSION   DBGJFU010000280 DBGJFU010000000
VERSION     DBGJFU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.286"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..634)
                     /locus_tag="ACOIAS_003829"
     rRNA            complement(<1..634)
                     /locus_tag="ACOIAS_003829"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          140 a          175 c          117 g          204 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccag
//
LOCUS       DBGJFU010000281          611 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.287, whole genome shotgun sequence.
ACCESSION   DBGJFU010000281 DBGJFU010000000
VERSION     DBGJFU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.287"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..>611
                     /locus_tag="ACOIAS_003830"
     rRNA            <1..>611
                     /locus_tag="ACOIAS_003830"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          126 c          179 g          134 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg c
//
LOCUS       DBGJFU010000282          577 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.288, whole genome shotgun sequence.
ACCESSION   DBGJFU010000282 DBGJFU010000000
VERSION     DBGJFU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.288"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..>577
                     /locus_tag="ACOIAS_003831"
     rRNA            <1..>577
                     /locus_tag="ACOIAS_003831"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DBGJFU010000283          577 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.289, whole genome shotgun sequence.
ACCESSION   DBGJFU010000283 DBGJFU010000000
VERSION     DBGJFU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.289"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            93..455
                     /locus_tag="ACOIAS_003832"
     CDS             93..455
                     /locus_tag="ACOIAS_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGZ2703458.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT          249 a           40 c           92 g          196 t
ORIGIN      
        1 tgttgaaagc ttataatgat tatgatataa attggttata gtatacaatt tatgggtata
       61 acaataaaaa attagaaagg aatttttata aaatgattaa attatatgga tatacaaaat
      121 gctctactgt taaaaaagct aaaaattggc ttaaggaaaa taatttagaa tttgaagata
      181 tagatatggt tcaaaaccct cctagtaaag aggagttaaa atcaatttat aagactagtg
      241 gatatgatat aaagaaattt tttaatacaa gtggtatgaa atatagagaa ttgggtctta
      301 aagatattgt aaaaactgaa tctgatgata aacttttaga aatattggta agtgatggta
      361 tgcttattaa aagaccatta cttttggatg gtaaaaatgt attattgggt tttaaagaag
      421 atgtatggaa aagtactttg ctaaaagaag attaggaaga gataggtgtg ttgattaaaa
      481 ttattaatta atacacctat aaatttatgt attaaatcta atctttataa atattaatta
      541 gcaaattcaa atcaaaaaaa ttcatatcaa caaacat
//
LOCUS       DBGJFU010000284          558 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.290, whole genome shotgun sequence.
ACCESSION   DBGJFU010000284 DBGJFU010000000
VERSION     DBGJFU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.290"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            3..263
                     /locus_tag="ACOIAS_003833"
     CDS             3..263
                     /locus_tag="ACOIAS_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703459.1"
                     /translation="MKNTKQITVEVNEILLQEMSKITNYENNILTDLINESIVNYILE
                     NKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
BASE COUNT          267 a           64 c           47 g          180 t
ORIGIN      
        1 tgataaaaaa tacaaaacaa attacagtag aagttaatga aattttatta caagaaatgt
       61 ctaagataac taattacgaa aataatattt tgacagattt gataaatgaa tcaattgtaa
      121 attatattct tgaaaacaaa aaacataata ttgaaaataa agatttagaa gaagtgatta
      181 aattttataa aaaagaaaat actgaaaaac ctaaaagtgc agcaagagct atagctgaag
      241 caaaaactca tactctgtat taatcaaata aaaaagttcc taaaaactag gaactttttt
      301 atattaataa ttattaatat atctcaatat ttatctcaaa tctatcaata taagcatgtt
      361 taatacaatc tattgtatac ttatcttacc ctagaaataa tcaaaacaaa aataaaatta
      421 tgccctttat atctaaaaca attaatttac atgattaata tacaaaaatt ctgactttta
      481 aattcgtata caatatcaaa ctaaaataat attaatgaca aacccaatat aaattaaatt
      541 caaacaggtt tgatttta
//
LOCUS       DBGJFU010000285          547 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.291, whole genome shotgun sequence.
ACCESSION   DBGJFU010000285 DBGJFU010000000
VERSION     DBGJFU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.291"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            173..271
                     /locus_tag="ACOIAS_003834"
                     /pseudo
     CDS             173..271
                     /locus_tag="ACOIAS_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            273..>547
                     /locus_tag="ACOIAS_003835"
     CDS             273..>547
                     /locus_tag="ACOIAS_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2703460.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNVLKDLDNAYKK"
BASE COUNT          233 a           52 c           87 g          175 t
ORIGIN      
        1 cttacggttc ttatatatgt tatttaaaaa taaatataaa aaaccttcaa aatacttgtt
       61 ttaagtaatt gcatatttta atattatgat aagtgatgaa aaatggaagt tactcatggt
      121 agaggatatg catatagtat gtcaaatata gacatccatt attatgggga gacatctttg
      181 gaatcctagt tatttcgttg ctactgtatt agaaaataca gaggagcaga taggaaaata
      241 tattaataat cagaaaaaac aagtaaagtg aggtgatagt agtggaaaaa gcttataagt
      301 ttagaatgta tccaaataaa aaacagcaag agttaattaa caaaactttt ggatgttgta
      361 gatttgtata taataagtat cttgctaaaa gaatagaagt ttataaaaac gataaagaaa
      421 cttttactta taaacagtgt agttctgatt tgaccaattt aaaaaaagaa ttaaagtggc
      481 ttaaagaacc tgacaaattt tcactacaaa acgtcttaaa agatttagat aatgcatata
      541 aaaaatt
//
LOCUS       DBGJFU010000286          527 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.292, whole genome shotgun sequence.
ACCESSION   DBGJFU010000286 DBGJFU010000000
VERSION     DBGJFU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.292"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..>527
                     /locus_tag="ACOIAS_003836"
     CDS             <1..>527
                     /locus_tag="ACOIAS_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HGZ2703461.1"
                     /translation="LDIKLIPKIIRLVNKTYIFLGILTIIVYMIIESIVTHLIINAVQ
                     KTKVKFIGIKMATMGFYYNLVTPFASGSQPMQIYALTKYDVSLSKSVAIVTNKTVVFQ
                     STVTVFCGILILLNKSLLMEQVHSVKVLITFGMIMNVSMLLGGLLIVFSPKQVKMIAE
                     VLINFLSKFKTFKFL"
BASE COUNT          201 a           55 c           79 g          192 t
ORIGIN      
        1 ttagatataa aactaatacc taaaattata aggctagtaa ataagactta tatatttctt
       61 ggcatattga caattatagt atatatgata attgaatcta ttgtaactca tttgataata
      121 aatgcagttc aaaaaacaaa agtaaaattt attggtatta aaatggctac aatgggattt
      181 tactataact tagttactcc atttgcatct gggagtcaac caatgcaaat atatgcactt
      241 acaaaatatg atgtaagcct tagtaaatca gtggctatag ttactaataa aactgtagta
      301 tttcaaagta cagttactgt attttgtgga attttaattt tgctaaataa gtcattactg
      361 atggagcaag tacattctgt gaaagtcttg ataacttttg gaatgattat gaatgtaagt
      421 atgttgttag gtggactact aatagtattt agtccaaaac aagtaaaaat gatagcagaa
      481 gttttgatta attttttatc taaatttaaa acatttaaat ttttaga
//
LOCUS       DBGJFU010000287          517 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.293, whole genome shotgun sequence.
ACCESSION   DBGJFU010000287 DBGJFU010000000
VERSION     DBGJFU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.293"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(92..385)
                     /locus_tag="ACOIAS_003837"
     CDS             complement(92..385)
                     /locus_tag="ACOIAS_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703462.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT          208 a           85 c           27 g          197 t
ORIGIN      
        1 aaaaaattgt agataaaact attttataaa atttatctac aattttttta tataaacata
       61 tatcaaaatc aaaatattat taatttatac tttaaagatt cccatcttta tcatatttta
      121 ttcttataag aaaatataat cctattacta ctaactctat cactaaaaat actccatagg
      181 taagaattga atcaatacta tctaacccat ttctaaaaaa aattaatgct atacctgctg
      241 ctataatccc tatagcattt tttcttactg cattttgccc aactccataa aaacctggca
      301 tttttccttt tatttcgaca gcagttaaaa ttatcccaaa cactataaat aatactccaa
      361 taagcatcat aatcatacta atcatctaat tacctccttc acttaaggaa atttcaattt
      421 ctaaaatcta aaataaataa tatttatttt ttataataat attgtatatc caattaacta
      481 ttttggaaat tagtaccaat aaataatgtt taaatat
//
LOCUS       DBGJFU010000288          514 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.294, whole genome shotgun sequence.
ACCESSION   DBGJFU010000288 DBGJFU010000000
VERSION     DBGJFU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.294"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            78..>514
                     /locus_tag="ACOIAS_003838"
     CDS             78..>514
                     /locus_tag="ACOIAS_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HGZ2703463.1"
                     /translation="MIAVKKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKG"
BASE COUNT          233 a           54 c           69 g          158 t
ORIGIN      
        1 attgttcacc acagcattat ataatatatt aaatattaaa atcattaaaa agtgtatcaa
       61 aaaacaatta aagtgaggtg atagcagtga aaaaagctta taagtttaga atatatccta
      121 ataaaaaaca gcaagagtta attaacaaaa cttttggatg ttgtaggttt gtatataata
      181 aatatcttgc taaaagaata gatgtttata aaaacgataa agaaactttt acttataaac
      241 agtgtagttc tgatttaacc aatttaaaaa aagaattaaa ttggcttaaa gaacctgata
      301 aattttcact acaaaacgcc ttaaaagatt tagataatgc atataaaaaa ttctttaaag
      361 aaaaggcagg atttcctaaa tttaaatcaa agaaaattaa tagattttca tataaaacta
      421 actttacaaa tggaaacatt atgtattgtg gtcaacatat aaaactacct aaacttggta
      481 tggttaaggt aagggataaa caagtaccta aagg
//
LOCUS       DBGJFU010000289          510 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.295, whole genome shotgun sequence.
ACCESSION   DBGJFU010000289 DBGJFU010000000
VERSION     DBGJFU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.295"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
BASE COUNT          157 a           67 c           35 g          251 t
ORIGIN      
        1 catataatct ttttaattca atttaaattt taaatatttt gtgtttatta tataatctga
       61 aaaaaaagca gtcaatagca tttttaattt ttttaatttt caaaattatg attcttaaaa
      121 aatcgttttc cttttgtcat agcacctatt tacgttaatt cttgtagtat tttgtattta
      181 ttttgctaaa ttgacagtta actttattcg ttttttattc tatttatgta gatttgcctc
      241 ttgtttttta gtcttaaaat cattttttct gaaaattcct tattatgatt tatcaactta
      301 tttttcttca tatcaattac tttctaactt ccaacttact taaactttgc agttgctagt
      361 attcacgttt acatacttat ctgtcataat taactaaaaa ttcttaatat atctctcaat
      421 gaaaacaatt tacccattta cttaatacat ttatataatc atatgtctta aattttgttt
      481 tattatttta ttattgtcac aattttattt
//
LOCUS       DBGJFU010000290          479 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.296, whole genome shotgun sequence.
ACCESSION   DBGJFU010000290 DBGJFU010000000
VERSION     DBGJFU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.296"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..>479
                     /locus_tag="ACOIAS_003839"
     CDS             <1..>479
                     /locus_tag="ACOIAS_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGZ2703464.1"
                     /translation="ATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVE
                     MEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTD
                     KPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRK"
BASE COUNT          197 a           60 c          116 g          106 t
ORIGIN      
        1 gcaacagatg gaccaatgcc acaaacaaga gagcatatac tattatcaag acaagttgga
       61 gtaccatata tagtagtatt cttaaacaaa tgtgacatgg tagatgatga agagttatta
      121 gagttagtag agatggaagt aagagattta ttaacagaat atgatttccc aggagatgac
      181 actccaatag taagaggttc agcattaatg gcattagaag atccaaagag tgagtgggga
      241 gataagatag tagaattatt cgagcaaata gatgagtata taccagctcc agagagagat
      301 acagataaac cattcttaat gccagtagag gacgtattct caatcacagg aagaggaaca
      361 gttgcaacag gaagagtgga aagaggagta ctaaaagtac aagacgaagt agaattagta
      421 ggattaacag aagcaccaag aaaagtagta gtaacaggag tagagatgtt cagaaaatt
//
LOCUS       DBGJFU010000291          429 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.298, whole genome shotgun sequence.
ACCESSION   DBGJFU010000291 DBGJFU010000000
VERSION     DBGJFU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.298"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="ACOIAS_003840"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="ACOIAS_003840"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="ACOIAS_003841"
     tRNA            310..398
                     /locus_tag="ACOIAS_003841"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
BASE COUNT          141 a           72 c           92 g          124 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgga tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggt
//
LOCUS       DBGJFU010000292          405 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.299, whole genome shotgun sequence.
ACCESSION   DBGJFU010000292 DBGJFU010000000
VERSION     DBGJFU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.299"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..215
                     /locus_tag="ACOIAS_003842"
     CDS             <1..215
                     /locus_tag="ACOIAS_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGZ2703465.1"
                     /translation="IGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSL
                     DLLSDNELKIIQGTIDGILNSRENKK"
BASE COUNT          125 a           73 c           68 g          139 t
ORIGIN      
        1 atatcggttc acacccaagt tttcaattat tccatgattt agttaccatg ttcaatatat
       61 cggttgatga atatttctat ccagcagaga aagttgcaaa aagcacagct cgccgtcaga
      121 tagaaacatc acttgattta ttgagtgata acgaattaaa aattattcaa ggtacgattg
      181 acggtatctt gaacagcagg gagaataaga agtaatcagc ttcttgtttt ccccgttttc
      241 tttttagcca tatttcttca agacttattt gttgatctgc tttgcaggaa acaaataaga
      301 gccgacaatc tataagttac aataacctac aaattatcgg ctctgcgtct tagcgtctgg
      361 ctctttgata atagttactt ttccatgctt tatattgatt aaagt
//
LOCUS       DBGJFU010000293          398 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.300, whole genome shotgun sequence.
ACCESSION   DBGJFU010000293 DBGJFU010000000
VERSION     DBGJFU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.300"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(3..>398)
                     /locus_tag="ACOIAS_003843"
     CDS             complement(3..>398)
                     /locus_tag="ACOIAS_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HGZ2703466.1"
                     /translation="NVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQ
                     VFLYMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLF
                     TFGTHYTLNSWKKENKVNSNLDKVIEKLN"
BASE COUNT          155 a           59 c           44 g          140 t
ORIGIN      
        1 tattaattta atttttcaat aaccttatct aaattagaat ttactttatt ttcctttttc
       61 caagaattta atgtataatg cgttccaaat gtaaataaac ctgaaactat taatataaaa
      121 tagtatacaa acccactata taagagtaca gagtacccca ataattcttt tggaattata
      181 ttggagaaca tagtaaaaaa tgtaacctca ttagctccaa catttcctgg tgttggaatt
      241 ggtgatatag ccatatataa aaagacctgt aatgtcatta tatgtacata tgaaactcct
      301 cttaaatttg aggctttata cacacaataa actatactga aatatgcgat taattgtaac
      361 acagtaagta atatacttaa aactaagatt cttacatt
//
LOCUS       DBGJFU010000294          389 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.301, whole genome shotgun sequence.
ACCESSION   DBGJFU010000294 DBGJFU010000000
VERSION     DBGJFU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.301"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..>389)
                     /locus_tag="ACOIAS_003844"
     CDS             complement(<1..>389)
                     /locus_tag="ACOIAS_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HGZ2703467.1"
                     /translation="LLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFLEMIDN
                     ENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPNITKYK
                     ENLINRIKMELSNADISIYNNNMKPLV"
BASE COUNT          135 a           45 c           40 g          169 t
ORIGIN      
        1 ctactaatgg tttcatatta ttattataaa tacttatatc tgcattactt aattccattt
       61 ttatgcgatt aataagattt tctttatact ttgtaatatt aggtatggat ttaataaaca
      121 ttcttgatac agaaggtttt ttatctacga tatgacttag tatactatct atatttattt
      181 caattttatt atcaatatcc cactgagcat ttgcagagat taataacaat ccccttgttc
      241 gaatatatga gttttcgtta tcaatcattt ctaggaatga gtcaaaaaaa caatatgtaa
      301 tatttgattt taaactttca ttttctaaga ataaaaaagt tttatatgct tcattatgat
      361 tttttgaata taatttatta ataagtaaa
//
LOCUS       DBGJFU010000295          381 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.302, whole genome shotgun sequence.
ACCESSION   DBGJFU010000295 DBGJFU010000000
VERSION     DBGJFU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.302"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(<1..>381)
                     /locus_tag="ACOIAS_003845"
     rRNA            complement(<1..>381)
                     /locus_tag="ACOIAS_003845"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           89 a          107 c           87 g           98 t
ORIGIN      
        1 tgtactaccc attgtagcac gtgtgtagcc ctaagcataa ggggcatgat gatttgacgt
       61 catccccacc ttcctccagg ttatccctgg cagtctctct agagtgccca acttaatgat
      121 ggcaactaaa gacaagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga
      181 gctgacgaca accatgcacc acctgtcacc aatgtccccg aagggaactc tccgattaag
      241 gagatgtcat tgggatgtca agcttaggta aggttcttcg cgttgcttcg aattaaacca
      301 catgctccgc tacttgtgcg ggtccccgtc aattcctttg agtttcactc ttgcgagcgt
      361 acttcccagg cggagtactt a
//
LOCUS       DBGJFU010000296          364 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.303, whole genome shotgun sequence.
ACCESSION   DBGJFU010000296 DBGJFU010000000
VERSION     DBGJFU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.303"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="ACOIAS_003846"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="ACOIAS_003846"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="ACOIAS_003847"
     tRNA            291..362
                     /locus_tag="ACOIAS_003847"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DBGJFU010000297          337 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.305, whole genome shotgun sequence.
ACCESSION   DBGJFU010000297 DBGJFU010000000
VERSION     DBGJFU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.305"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            123..>337
                     /locus_tag="ACOIAS_003848"
     CDS             123..>337
                     /locus_tag="ACOIAS_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGZ2703468.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAEGDTNAGNA"
BASE COUNT          140 a           32 c           75 g           90 t
ORIGIN      
        1 gtatatatta aaaattatta gagtgcatat aaggatgtat acagtgtata ttaagaatta
       61 ttagagtgta tataaggatg tatacagtgt atattaaaaa ttattaaggg gagggggaat
      121 ttatgcaaca aaaatgtaaa aagaaagcac tatcaatgat tttaagtatc tcaatgggac
      181 ttggatcagt agcatcattt aatgcgtcaa atactttcgc agatactata gaagaaagta
      241 ctaatgcagg aaatgcagaa ggagatacta atgcaggaaa tgcagaagga gatactaatg
      301 caggaaatgc agaaggagat actaatgcag gaaatgc
//
LOCUS       DBGJFU010000298          304 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.309, whole genome shotgun sequence.
ACCESSION   DBGJFU010000298 DBGJFU010000000
VERSION     DBGJFU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.309"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..>304
                     /locus_tag="ACOIAS_003849"
     CDS             <1..>304
                     /locus_tag="ACOIAS_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HGZ2703469.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPELNAKVSYAVEKADCYTGNTIESER"
BASE COUNT          119 a           44 c           55 g           86 t
ORIGIN      
        1 ctaatgtaat ccaaaagata caacaattag tacctccaac tcatccagtt ttaggtgatg
       61 gaatattagt tttaacagat ataaaatctt ctccatatcc aggagcatca gtagtgccag
      121 attattgcaa agctactttt gatagaagat tattagttgg agaaacaaga gaaggcgtat
      181 tagctccaat acaagcttta ttagatgaaa tgatgaaaga agacccagaa ttaaatgcaa
      241 aagtttctta tgcagttgaa aaagctgatt gctatacagg aaatactata gaaagtgaaa
      301 gatt
//
LOCUS       DBGJFU010000299          303 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.310, whole genome shotgun sequence.
ACCESSION   DBGJFU010000299 DBGJFU010000000
VERSION     DBGJFU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.310"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..>303
                     /locus_tag="ACOIAS_003850"
     CDS             <1..>303
                     /locus_tag="ACOIAS_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HGZ2703470.1"
                     /translation="KILINIICIIALTFSSLSIYIKLSEYKKADEVYTELRENTINNS
                     KHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFMDYRN"
BASE COUNT          126 a           40 c           34 g          103 t
ORIGIN      
        1 aaaatattaa taaatattat ttgcattatt gcgctaactt tctctagcct ttcgatttac
       61 ataaaattat ctgaatataa aaaagctgat gaagtatata cggaactcag agaaaacact
      121 atcaataata gcaaacatca agaactttat gataaaaata atgattatag attctggctt
      181 aaaataaaca acgtaaatat aaactatcct gtagtacaag gatataacaa tgatttctac
      241 ctaactcatg atttttataa aaattatctt cctttaggta gtattttcat ggattataga
      301 aat
//
LOCUS       DBGJFU010000300          298 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.312, whole genome shotgun sequence.
ACCESSION   DBGJFU010000300 DBGJFU010000000
VERSION     DBGJFU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.312"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
BASE COUNT          117 a           38 c           41 g          102 t
ORIGIN      
        1 tatgtaagaa ccgtaggaac tacggggatt gcctgtggag aattagtaag acatatttta
       61 gtatgcaaaa ttctatgaag caggaaccct gtgactttag tcatgggagg ttcagtttag
      121 cttaaaactt acttatctat ttcagtttct attagaatta ttattagact cattaaaata
      181 aaagcactaa tataactaat aatatatgtt aaattcatta actctacata aagttttaca
      241 atctaaatga aatacaataa tctgcataaa attcaatagc ttatataaaa tttaataa
//
LOCUS       DBGJFU010000301          266 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.denovo.314, whole genome shotgun sequence.
ACCESSION   DBGJFU010000301 DBGJFU010000000
VERSION     DBGJFU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.denovo.314"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..>266
                     /locus_tag="ACOIAS_003851"
     CDS             <1..>266
                     /locus_tag="ACOIAS_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2703471.1"
                     /translation="YKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVYKSDKETF
                     TYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKE"
BASE COUNT          121 a           27 c           35 g           83 t
ORIGIN      
        1 tataagttta gaatgtatcc aaataaaaaa caacaagagt taattaacaa aacttttgga
       61 tgttgtagat ttgtatataa taaatatctt gctaaaagaa tagatgttta taaaagcgat
      121 aaagaaactt ttacttataa acagtgtagt tctgatttga ccaatttaaa aaaagaatta
      181 aaatggctta aggaacctga caaattttca ctacaaaacg ccttaaaaga tttagataat
      241 gcatataaaa aattctttaa agaaga
//
LOCUS       DBGJFU010000302        11385 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.guided.121, whole genome shotgun sequence.
ACCESSION   DBGJFU010000302 DBGJFU010000000
VERSION     DBGJFU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.guided.121"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            227..844
                     /locus_tag="ACOIAS_003852"
     CDS             227..844
                     /locus_tag="ACOIAS_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HGZ2703472.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            952..3483
                     /locus_tag="ACOIAS_003853"
     CDS             952..3483
                     /locus_tag="ACOIAS_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGZ2703473.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3917..4618
                     /locus_tag="ACOIAS_003854"
     CDS             3917..4618
                     /locus_tag="ACOIAS_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HGZ2703474.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4668..5750
                     /gene="vanS"
                     /locus_tag="ACOIAS_003855"
     CDS             4668..5750
                     /gene="vanS"
                     /locus_tag="ACOIAS_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HGZ2703475.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6011..7111
                     /gene="vanG"
                     /locus_tag="ACOIAS_003856"
     CDS             6011..7111
                     /gene="vanG"
                     /locus_tag="ACOIAS_003856"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HGZ2703476.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7108..7914
                     /locus_tag="ACOIAS_003857"
     CDS             7108..7914
                     /locus_tag="ACOIAS_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HGZ2703477.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7932..10070
                     /gene="vanT"
                     /locus_tag="ACOIAS_003858"
     CDS             7932..10070
                     /gene="vanT"
                     /locus_tag="ACOIAS_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HGZ2703478.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10384..11004
                     /locus_tag="ACOIAS_003859"
     CDS             10384..11004
                     /locus_tag="ACOIAS_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HGZ2703479.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11170..>11385)
                     /locus_tag="ACOIAS_003860"
     CDS             complement(11170..>11385)
                     /locus_tag="ACOIAS_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGZ2703480.1"
                     /translation="FSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTN
                     SKTEKFISKLLNFIVIFLIGYQFYILL"
BASE COUNT         4321 a         1189 c         2020 g         3855 t
ORIGIN      
        1 ttcatgaggc actctttttg tatgaaatca aatatatttt tattatttca acaataaaaa
       61 agaggtctgt ttctattttg agacaatatt atttttatgt agatttaatc ttaatgagta
      121 taaagtttta aaactactaa aataggagga attttaatat aaaagggtaa ataaagggtt
      181 tagagggcat aatatagaat aaattaatta tttaaggagg gtttttatgt gcaaaaaaat
      241 gtatttatta gtggtaggta tacttatagc aggtttatta actgcatgta atgggagtcc
      301 aactaaggat tcagaacaag cacaagataa tacaaaaaag gaggcaaatg tatcaaataa
      361 cgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa caagatttta aagtgccata
      421 cacagatgca ataaacatat ttaaagacaa atacaaagat gctgatatag tagatttaag
      481 tcttgagaga gatttaaata aatttgttta tactgtggaa ggtgtagatg ataataatga
      541 atataagatg aaaattgatg ctaatacaaa agatgtatta gaagataaaa cagaaaagtt
      601 ggattcagaa gatttaaatg gagtcgctag aaaagaaaaa ttagatttta atgacataat
      661 gacacctcaa caagctatgg aaattgcatt aaaagaacaa aatggaatgg ttaaagagtg
      721 gagtttagat aaagatttag atgttacttt ttataaaata agaatagata aagataaaaa
      781 tgaatatgac ataaaagtag attctaaaaa gggaactgta ttgaaggtag aaaaagaaga
      841 ttagaagatt taaactatct ttaaaataat ttcttttttg aataaaattt gtttaaaata
      901 taacaagtag ggtataaaga gactaaataa ctattttagg aggagtacgg tatgtcattg
      961 aaatcgctaa aaataaaaga aaatttatat tgggtaggtt cgcttgaccc tgatttaaga
     1021 gtatttgaca taattatgta tacaccttat ggaactactt ataattcata tgtattaaaa
     1081 ggaacagaaa aaacagtttt atttgagaca gttaaagata agcattttga caattatatt
     1141 gaaagattaa atgatttaaa tatagatttt gaaaaaatag actatattgt tgtaagtcat
     1201 actgaaccag accatgcagg aagcgttgaa aagcttttgg atttagctaa aaatgcaaaa
     1261 gtagtagctt cagaaacagc aattaaatat cttaaggaaa tcgtaaacaa agactttgaa
     1321 tatgttgcag ttacagatgg agatacatta tcaattgggg acaaaacttt agaatttttc
     1381 tctgtaccta tgcttcattg gccagatacc atatacacat atataaaaga ggataaaact
     1441 cttgtaacat gtgattcatt tggtagtcac tatagtaatg ataagatagt aaatacactt
     1501 gatgaagatg aagaaaagga ttacttggat gctttgagat actattatga ttgtataatg
     1561 ggaccattta aaccatctat ggtaactgcc atagaaaaga taaaagattt agatatagat
     1621 actgtatgtc caggtcatgg tccagtatta actgaaaatc ctagaaaaat tatagacctt
     1681 tattacaact ggagtgtaaa tgaacaaata aagttagaaa aagaagttac aatttgttat
     1741 gtttctgcac atggatatac aaagattatg gcagaagcta taaaagcata tattgaaaaa
     1801 aatagcaact ataaagtaaa tttatttgat gtaatagagc ataagcaaga agatattttg
     1861 gcaaaaatag cagtatcaca aggtgtatta tttggaacac caacaatatt aggagatgcg
     1921 ttaaagccta tatgggatat attaatatcg ttaaacccag ttcttcatgg tggaaaagtt
     1981 gcttcagtat ttggttcata tggatggagt ggagaaggta tagaaaatgc tatggagaga
     2041 ataagtcagc ttagaatgac agcagtaaaa ccttttgctg ttaactttaa accatcaaat
     2101 gaagagattg ataaattaca ttcttataca ggtaaattcc tagataagtt aaattctact
     2161 tttggaagta aaaagaaaac taagaaattt aaatgtgtaa tttgtaatga agtttttgaa
     2221 ggtgatagtg ctcctagtgt atgccctgta tgtggagcta aggaagacca atttatagaa
     2281 gttgaagaag atgaagttac ttttagaaaa gatactgatg aatatttcgt aatagtagga
     2341 aatggagcag ctggttttta tgcagcagat gctataagaa aaagaaataa aacttgtaag
     2401 ataactatga tttctaatga agatgagttg acttactata gacctgcact ttctgatggt
     2461 ataaatgaag aacttggttc agatttctac atggaagata aagattggta tgataaaaat
     2521 aatatagtag taatattagg aacgaatgta gataaattag atgaagtgaa taaaactatt
     2581 atagtgaatg atggagctat taaatttgat aagcttgtaa tagctacagg aagtagaaac
     2641 tttataccac caattaaggg tcatgattta gaaaatgtgt ttactcttag aaatataaaa
     2701 gatttatata gtgtaaaaga agcactagaa aaatctaaga aagttgtagt gattggtggc
     2761 ggtttattgg gacttgaagc tgcatgggaa tttagactaa aaggattaga agtagtagta
     2821 gttgaagcca tggatagcat actatcaaaa caacttgata aagaaggcag taaaatatta
     2881 gaacaatgtg taagagatac aggaatagat gtaagattgg gtgtagcagt tgatggtatt
     2941 gaaggtgatg gaaaagctca aaaagttgta tttaaagatg gagatagtgt tgactgtgat
     3001 atggtagtat tttcaattgg tgtaagagca aacactcaaa tggttcagga tacatctgta
     3061 aaaattgaca gaggtattgt tgtagataag acattacaga caaatgttaa agatatatat
     3121 gcttgtggag atgttgcaca agtgggaaat attagtttag caatatggcc atcttcagta
     3181 gaaatgggaa aaatagcagg ggcaaatgca agtggagata atttgacatt tgaaagtgaa
     3241 gtatatccag tttcattaga tgcaatgaat gtaaaagtat ttagtattgg taatatacaa
     3301 aattttgata aagaaatttc atcaaaagat gaaggtcaaa gaatatataa aaaattattt
     3361 atgaaagatg gttcattagt tggagctata cttataaatg atttaagctg tactgttaaa
     3421 ttgataagat taatatctga aaaaggtgat tttgaggata ttatgaaggc tgatatacta
     3481 taatttaaat acattaaaat aaaaaggagt tgtcttaaaa ttgatttttt tctaatcaaa
     3541 ttttaaattc aactcctttt ttgaattttt atataaaata acatggaaat tttagtaatg
     3601 tctgtggtta catacatagg tttaattgtt ttttatatgg attgtctctg gtataataaa
     3661 ttgaattgtg tttgactgat agatattttt attgaaagtt aatacctgtt atgcgataat
     3721 aaggacgtgt taggggagtt tttataataa caattaatct cagtttttgt gttccaatag
     3781 tagcatcaac acattctgat gtagtagttc ttttcttata aaaattagtt acaaatatgc
     3841 ttgtaattat ttgatatgta aatttaccaa tattataaat gatgacctcg ataataagga
     3901 gagtgaaaaa taatctatga acgaaaatat tttgattgtg gatgatgaaa aagaaatagc
     3961 agatttaatt gaagtatatt taaaaaatga aggttttaca gtatacaagt tttatacagg
     4021 taaggaagct ctagaatgta ttgaatctaa aaatttggat atggctattt tagatattat
     4081 gcttcctgat attgatggat ttcatatttg ccagaaaatt cgagaaaaat actattatcc
     4141 tatcattatg ttaacagcta aaattgaaga tatagacaag ataatgggtt taaccatagg
     4201 tgctgatgat tatataacaa agccatttaa ccctttggaa gttgttgcaa gagtaaagac
     4261 acaattaaga agatatatgc gatataataa ttcttatgaa caacagtcga taattgttaa
     4321 tgagtatgat attaaaggat tgattatcaa taaagaaaca cataaatgta gtttatttgg
     4381 gaaagaagta gcattaacac cgattgaatt ttctattctt tggtatttat gtgaacatca
     4441 aggaaaagtg gttccttctg aagaattgtt tgaagctgta tggggagaaa aatatcttga
     4501 taataataac acagttatgg ctcatatagg gcgattacgt gaaaaaattc atgaaccttc
     4561 taaaaacccg aaatttataa aaactgtatg gggagtaggt tatacaattg aaaagtaaaa
     4621 atattaaagt caatcctgac tttactcaag tgagaaataa aatttttatg agaacagctt
     4681 taatggcatt tatagccatt attttcatat ggattttata ttcttttata ttgtatggta
     4741 agttttctat ctggatagta tcattctttg aaaatgcttt tcgtatggaa tataaagctg
     4801 cattgaattt atatcagcag attttccgta atagtatgga gctcttcatg atactagcta
     4861 tggtgattgt gttttttata atattccgtg tttatttaaa ttggtttaca aaatatttta
     4921 gtgaaataaa tcaaggtatt gactctctta tcaaagaaga tgttggagaa gtggctttat
     4981 ctccagagtt attggcaata gaaaagaaaa taaactcaat taaacatata cttgaacaac
     5041 gtaaatttga gacacaaatg gcagaacaac gtaagaatga acttattgtt tatcttgcac
     5101 atgatttaaa aacaccactt acatcagtta tagggtatct gacactactt cgagatgaaa
     5161 gtcagatttc tgaagaactg cgaaaaaaat atttatctat ttcgttggat aaggcagagc
     5221 gtcttgaaga tttaattaat gaattctttg aaattacaag gtttaattta tcaaatatat
     5281 cacttgaata cagtacagta agcttaactc gtatgcttga acaactaaca tatgagttta
     5341 aacctacttt gatgagtaag aatttaaaat atacactgaa tgttacacca aatatgatga
     5401 taaggtgtga tgttaataaa atgcaacgtg tatttgataa cttattacga aatgctgttt
     5461 attatagttt tgaagatact actattgaaa taacagctgt acaggaggat gattatataa
     5521 agcttaaatt tataaatcat agcaatacga ttccagagga aaaacttgag cgtatttttg
     5581 aacaatttta caggttggat acatctcgta attcaaacag tggtagagct gggttgggac
     5641 ttgctatagc taaagaaata gtggagcttc ataacgggac aataacagct catagcgaga
     5701 acaatataat tacctttaag gttattattc cgattgtagg aaatttgtaa gaatttaagt
     5761 ataaaaaaat aggaaattct ttaggataaa taaataaaga gcactcttgt tattgatata
     5821 attactgtat caaaacaaga gtgcttttat tttgatacag taattatatc aatagaaaaa
     5881 tgtgaaaata tattatgtgg aacagatgca attgtaagtg aaggttgatt ggatttgaaa
     5941 tagaaaaagt attttaacat gtttctgtag attgtataaa aataaataaa tcaacaaagg
     6001 agaatcaaag atgaataata aaaaaattgc agttatattt ggtggaaact caacagaata
     6061 tgatgtatca ttacaatcag cttttgcagt acttgagaat ttgaatacgg aaaaatatga
     6121 tattatacca attggaatta caagggaggg taattggtat cattatattg gagattataa
     6181 taatatccag aataacactt ggtttgaaag ggatactgat ataattccag ttattgtttc
     6241 gcagaaccgt gtcaaaaatg gtattattga atttttaatt cctgatgctg tttctcagaa
     6301 tcatattcca aatagtgcta ttgaatcatg tgaaaatgat attaaataca ctaaacttga
     6361 ttttgtgttt cctgtactgc atggaaaaaa tggagaagat ggaacagtgc aggggttatt
     6421 tgaattggca ggtatacagg ttattggatg tggtactctt tcttcagcat tatgtatgga
     6481 caaagacaaa tcacataaac ttgttcattc agaaggtatt aaagtaccaa aatcaatatt
     6541 actaaaatac tcagataaat ttaatgtggc tgaactgtca aaagacttaa cttatccatt
     6601 atttataaaa cctgtgcgtg caggttcatc atttggtatt agtaaaattt ataaaaaaga
     6661 agagttacaa gtagcagtag acttggcttt tgaacatgat aatgaagtaa taattgagga
     6721 aaatattgat ggttttgagg tagggtgtgc agttttagga aacaatgact tacttgttgg
     6781 aagagtagat gaaatagaat tgtcaaaagg attttttgat tatacagaaa aatatacatt
     6841 gaaaagttca aaaattcata tgccagctag aattgatttg gattgtgaga aaagaataca
     6901 agagacagca aaaattattt atagaactct tggatgttct ggatttgcta gagtagatat
     6961 gtttttaact ccatctggtg aaattatttt taatgaagtc aatacaattc ctggctttac
     7021 ttctcatagt cgttatccaa atatgatgaa aggtattgga ctgtcttttg aagaaatatt
     7081 ggacaagata attgaggtgt acataaaatg atgaataata ttttattaga taaaaataaa
     7141 gaagataaaa tgacaaatac tatcttattg actaaagata aagtttatga tgggaatcta
     7201 attctagtaa atgctttttt accagttaaa acatctgaag atatagattt aattcctgta
     7261 gatacaaggt ttccaagtat tcttatgaaa cgtgaagcta caaacatttt acaaaatatt
     7321 ttaaaaagta tatgtggaat aaatgaaata gtacctgtaa gtggatatcg tactgcagag
     7381 gaacaacaag atatatattc aagttctctg cgtgacaatg gaaaagactt tactaaaaag
     7441 tttgtagcac ttccaaatca cagtgaacat cagacagggc ttgctattga tttagggtta
     7501 aagaaagaag ttatagattt tatctgccct gaatttcctt ataatggtat ttgtaatgag
     7561 tttcgtaagg cagcactaca ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa
     7621 ataacaggaa tatctcaaga accatggcat tttcgttatg tgggatatcc gcattctgag
     7681 attatcaata aaatgaatct cgctcttgaa gaatatatag aatatattaa gagattttcg
     7741 tatacaaatt gtttaaaaat agaagataaa gaaaaaatta tagaaatctt ttatgtgcct
     7801 attcatacta aagaagaagt gagaatctct atacctgaac aatgtgtata tcaaatatcg
     7861 ggtaacaata aggatggttt tattgtgaca ttttggagaa atagaaagga ctaagaaatg
     7921 gaggaacaag aatgattagg aataaatctt atgcagggct tgattatttt aggattatag
     7981 ctgcattact tataattgca attcatacat ctccattatt atcatatagt aaaatggctg
     8041 actttgtgtt aactagtata attgctcgta ttgctgtgcc attctttttt atgctatcag
     8101 gcttttttct aatttcaaca tataattata attgtaataa actgaaatct tttctaaaaa
     8161 atgtattatt tatttacggg atagctattg taatttatat tcccttaaat atttacactg
     8221 gatatttttc catgaataat ttagtgccca atattattaa ggacattgta tttgatggga
     8281 ctttatatca tttatggtat cttcctgcat caattattgg tgcaagtatt gcatactttc
     8341 ttgtaaaaaa actaggttta aagtactcac tagtaatcac tattgttatt tatgtaattg
     8401 gtatgtttgg tgatagttat tatggttttt tagaaaaaat accactatta aagaatttat
     8461 atgggtatat ttttgaagta tcagattata cacgaaatgg tatatttttt gcacctgtat
     8521 ttttcatact tggtggaata atatctaata aatctaatta tatgtcaact aaaaatagct
     8581 tgattggatt tcttatatca ttcttactta tgttatgtga gggaatgttt ttacataaac
     8641 taggggtaca gcgtcatgat agcatgtata ttatgttatt gccatgtatg tatttcttat
     8701 ttacagcctt aacatttttg aaaggttctc gagctaggtt attgcgaaca tcatcattaa
     8761 ttatttatat tatacatcct atgatgattg ttgttattcg tatgttttct aaaatattcg
     8821 gaatacaaac tgtcttggtt gacaatagcc ttattcatta tatagtggta agtatagcat
     8881 cttttatagc ttctataatt attatattga ttcttaaacc tctaaaaaaa gacttgaaag
     8941 gagaacataa ggtaaatacc aatagagctt ggattgaaat tgatttaaat aatttggaac
     9001 ataatgtaaa gatgatacaa aaagctatgc cagaagattg tgaattgatg gcagttgtaa
     9061 aagctaatgc ctatggtcat agtgcttttg aagtagcgac ttgtgcaaac caaataggtg
     9121 taaaagcatt tgcagtagca acaatagatg aggggattga tttacgtcga tatggaatac
     9181 taggagaaat attgattctt ggatatacta acccaataag ggcaaaggaa cttcataaat
     9241 acaatcttac acagagtgtt attgacctta atcatgctat ctctttaaac aaacagaaat
     9301 acaacataaa atcacacatt aaaattgata caggtatgca cagactgggt tttgatgtta
     9361 atgatgtgag gtcaatttta aaggcattta aattaaaata cttagatata tgtggcatat
     9421 ttacacactt gtgtgtttca gatagtctga aagatgaaga tgtagacttt acaaataaac
     9481 aaattgaatg tttttataag ttaattgatt ttctgttaaa aaaaggtata aaaattccta
     9541 aagtacatat tcaaagtagt tatggttttt ttaattatcc caatcttaag tgtaattatg
     9601 taagaattgg aattgcatta tacgggatat taagttttcc aaatgatacg actaaattgc
     9661 aacttgattt acgacctgtt ttatccttaa aatcacaagt aattcttatt agaaaaatac
     9721 aaaaagatga tagttttgga tatggaagag tctttattgc aagcagagat agcgtaattg
     9781 ctatattatc tattggctat gccgatggta tacctagaaa tttatcctgt ggaaaaagtt
     9841 acgtaataat aaatggttgt agagcagcaa tagtgggtcg aatttgcatg gaccaacttg
     9901 ctgttgatat tacagatata cctaatgtag aagttggtaa tacggcaata ataataggaa
     9961 gagataattt atctgaatta tctgtttctg aagttgcaaa taactcatgt agtatatcga
    10021 atgaattatt gagcagagta ggaagaaggt taaatgttat aaagaagtaa ctcaattaaa
    10081 tagtaatatt tgttgtactg tgtaatttat tttactctga ctagacattt ttagtaatat
    10141 tatgtctaat atatattttt tataacttac atgacaattt aagttataaa aaggtgatgt
    10201 ttcagatttt tattttgaaa catcaccttt tttgttataa tatataataa aataattata
    10261 ccttaactgt aaacttatat agattattta ctttttaata ttagattcat tttttgagat
    10321 tggtatataa taaacatata gagtatataa taatagttaa agttaaaaaa ggggaaaagc
    10381 aatatgcagt atatacaaga actacttgat ttgactcaaa attattgggt gttagccaca
    10441 ataatcggct tattaagtgc atttatagaa agctttatac cagccttacc acttgttgct
    10501 atagttacag caaatgctgc aatacaagga ttatttattg gatgtttgtt atcatggatt
    10561 ggctcaggac ttgggacaac ttcattattc ttattaataa gcagatttaa tgatagtaag
    10621 ctttttaata agttgagaaa tagtaaaact gaaaaagcaa taagttggat ggataaacag
    10681 ggatttaagt tattatttat agcgtatgcc tgtccattta tgcccggttg tttagttact
    10741 atcgcatcag cttttggtaa aaaagattta aaagattttg tgccagcaat gttggcagga
    10801 aaatttgtaa tgtttatagt tataagttac gtggcaagtg atatagaagg ttttataaca
    10861 agtcctttaa agatagcatc atttatatta ttggtgtttt tgtcatggaa gattggaaat
    10921 agagtaaata aaaatcttga aaatcataat tatgatttcc atcataaaaa acatcacaat
    10981 gataaagatg ataaaatgat ttagcttgta cgaaataaaa cgtatcttaa aatgatttag
    11041 tactaaatta ttttaagata cgttttattt tttgtattaa ggtacaaaat ttttagttct
    11101 tttgtaaact aaaatatata aaacttattt atacttagtt caaaatattg ttatttattt
    11161 tttctacaac tataataaaa tataaaattg atatccaatc aaaaaaataa ctatgaaatt
    11221 taatagctta gatatgaatt tttctgtttt tgagttagtt tttataggta gtaaagaaat
    11281 gtttatagta atgggataaa acaattcaat accttcttta gtaataaggt caagacacaa
    11341 gtgagataga tatcctatta taaacccact ggacaaacat gagaa
//
LOCUS       DBGJFU010000303         9148 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.guided.145, whole genome shotgun sequence.
ACCESSION   DBGJFU010000303 DBGJFU010000000
VERSION     DBGJFU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.guided.145"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            215..907
                     /locus_tag="ACOIAS_003861"
     CDS             215..907
                     /locus_tag="ACOIAS_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2703481.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            904..1839
                     /locus_tag="ACOIAS_003862"
     CDS             904..1839
                     /locus_tag="ACOIAS_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2703482.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            1987..2910
                     /locus_tag="ACOIAS_003863"
     CDS             1987..2910
                     /locus_tag="ACOIAS_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703483.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            2894..3664
                     /locus_tag="ACOIAS_003864"
     CDS             2894..3664
                     /locus_tag="ACOIAS_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703484.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            3674..4456
                     /locus_tag="ACOIAS_003865"
     CDS             3674..4456
                     /locus_tag="ACOIAS_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703485.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            4833..5369
                     /gene="rbr"
                     /locus_tag="ACOIAS_003866"
     CDS             4833..5369
                     /gene="rbr"
                     /locus_tag="ACOIAS_003866"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HGZ2703486.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            5401..5790
                     /locus_tag="ACOIAS_003867"
     CDS             5401..5790
                     /locus_tag="ACOIAS_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HGZ2703487.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            5827..6213
                     /locus_tag="ACOIAS_003868"
     CDS             5827..6213
                     /locus_tag="ACOIAS_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HGZ2703488.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            6340..7782
                     /locus_tag="ACOIAS_003869"
     CDS             6340..7782
                     /locus_tag="ACOIAS_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HGZ2703489.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            8079..8894
                     /locus_tag="ACOIAS_003870"
     CDS             8079..8894
                     /locus_tag="ACOIAS_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGZ2703490.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         3220 a         1173 c         1755 g         3000 t
ORIGIN      
        1 attttttcac aatgatgaaa tatactaatg ggtgttatag gtataagaat cgcattacac
       61 ttgaagaagt aagtgataaa tgagatataa atcctcatat gataaactac tatttcattg
      121 aggaacatat tctaagtgat gtcaagatac tacaatatga ctaaattcct agtgataaaa
      181 aagtctacga atgagtgtag aaaggggaac taagatgaat aaagttttaa ttatagatga
      241 tgataaagat ctttgtattt tgttaaaaaa gagtatttca attgaacaga ttgaagcaaa
      301 ctattgccat tctgggagag atggactggt tgaactttca aaacaagatt atcagttagt
      361 agttctagat gtgatgatgc caggtatgga tggttttgaa actatggaac aaatacgaaa
      421 atatagcagt attcctatct taatgctgac atcaaaaagt gataattaca gcaaagtgca
      481 tggattgcga gctggtgcag atgactatct aaccaaacca tttgaaatta cagaatttac
      541 tgctcgtgta ctgtctttaa tccgacgtta cacccgtttt aatccaaaaa atgaggagcc
      601 acagcaactt tctttcaaag gtttgttgat tgaccttgac cagcatacag ttactaccaa
      661 tggcgaacct atgttgcttc cacccaagga atttggtgta cttctttact gtgcaaggca
      721 tcagggtaag attttgacaa aacagcagat ttatgaagcc gtgtggggtg agccatatgt
      781 atatgatgac agcaatatca tggctattat cagtcgccta agaaaaaaaa ttgaactaga
      841 cccatcatct cccatctatt tgcaaacaat aaaaggaatt gggtatcaat tcaacacgga
      901 ggtatgaaaa tggatgaaca aacgataatt cttttcttac tcttgctgtc gattggtcta
      961 ctttttcttt ccatatttgc tttttccaag cttgggcata ttcacaaacg cttaaaagat
     1021 atagaggaga tactcgctga tgtacaaatt ggagacgaaa atcgtaaaat actaattaag
     1081 ccatgtgatg ttatggctcc attggtttat cagcttaatg agattgtcta cgattatgaa
     1141 aataagttac tttctctgaa aaagtcggat aaagctagca aacaacttat gacaagttta
     1201 tctcatgatg ttcgtacacc attaacaact ctgattggtt atctggatgc tgtacacagt
     1261 ggtatagtta tcgaacaaga gcgggaggaa taccttgaaa tcgctaggcg tagagcctac
     1321 gacctcaagg attacattga tgttcttttt gactggtttc ggttgaactc tgatgaattt
     1381 acgctgtcaa tagaatctgt ggaaattgca gagttgtcac gcaatatttt gaaagactgg
     1441 attcctatat tccatgaaaa gaaactgaat tttgaaattg atattccaga aaatcgacta
     1501 atggtaaacc tcgacccaga tggatattcc cgtgtggtca ataatctggt tcaaaatgtt
     1561 cttgctcaca gtaaggctcg gcaaatcaag attaccatga gtgaggattc cagaatggta
     1621 ttactccgtg ttgaggatga tggtgttggg attgccagag aaaatttacc acatattttt
     1681 gagcgactgt ataagtgtga taaaggacgt tcagaaaaag gaagtggtct tggtctatct
     1741 attgtcagtc aaatggtgga gcgaatgggt ggtcaggttt ccgtagaaag tgagattgga
     1801 aagtataccg tatttacagt gagcttacca ttaatataaa attgtttttc ccccatgtga
     1861 catgtataca cagggggatt ttttgtaagt tataaagttg caaggttaat acaaggttgt
     1921 tgcaaggtta atgtaagatt gaggtggtat gataacttta ataaacaaag gaggctttta
     1981 ttatttatga ataatttaat tattgaaaca aaaaatctca ctaagcaata tggtgctcaa
     2041 aagagcgttt ctaatttgaa tattcacgtt cagaaaggtc gcatttacgg attgctagga
     2101 agaaacggtg cgggtaagac gacaactatg aaaatgttat tgggcctaac aaaaccaact
     2161 actggagaaa ttaaaatatt tggtcaggac attcacaata acgagaagaa gcttcttcct
     2221 cgaattggat gtttgattga atcaccaggg ttttatccta atctgacagg aacagaaaat
     2281 ctcaaaatct ttgcaaggct tagaggaata ccaaaaaaaa atgcagtaga atcagcactg
     2341 gatgtagtcg gtttgccaaa caatgacaaa aagttatttt ctcaatactc tcttggtatg
     2401 aaacagaggt tggcgattgc tcttgccatt atgcatgacc cacaactgtt gattttggac
     2461 gagcctatca atggacttga tcctattggt attgcagaag taagaacgtt tattcgtgat
     2521 ctctgcacag aacgaggtaa aactattttg atttccagtc acattctttc tgaaattgcc
     2581 ttgttggcag atgacattgg cattattgac catggtattt tgttggagga agaaagtctt
     2641 gcccaattag aagaaaaaaa cagtaaatac atacattttg ctgtatctga tgcagcacag
     2701 gcagcaaggc ttctggaaac ccaacttaac ctgaaaaatt tcaaagtagc agataattac
     2761 aatattatgc tatatgatac agatgtttct gttgcagaaa taaatcgtgc tttcattcta
     2821 aacaacattg aggtatcaga atctcattta tgcgaagata ctcttgaaga ttacttcaag
     2881 aaagtaacag ggggtgaagg aattgcttga tataatttca tgtgaatttt caaaactcaa
     2941 gcggttaaaa tttatactga tttctatttt aggtgcttgc ttatttccaa ttcccgcaac
     3001 aattttgatt gcaaaggaca acttgccctt tgagcaattg ttcaaattag ttgtcaattt
     3061 tggatatttc ttgctattac caattgtatt aagtattgtt gctagccaac tattttttat
     3121 agaaagagat aatgatgtat taaaaaatct tgcaacagtg ccagtgccaa aaggaaaact
     3181 ggctctggca aaattggctg tgttgctgtt tatatcattg ttctactctg ttgcagggtt
     3241 aggagctact atcattggag gtttcattgt tggaatagta gagggggttg ctgtaaaatt
     3301 aggaatgagc attgcttttg gaattatgtt ttttgttact gtacttcctg ttgtggtact
     3361 cattgtatat ttcaatcgca gctatatttt ttctattata ctttcttttg tatatgctat
     3421 tttcaatttt agtatatcct taaatattat caattttgaa ccaaataacc cactattaag
     3481 cgtattacct gctcctgtta taatgcgttg gtggatggca ttttggggag atcccactgg
     3541 cgaatacacg gtacttagac agccatattt gctctcaact cctgcttgtg taggtatttt
     3601 atgtttgata accattatag cagtcctgct gatttgtatg atatacaaaa agcaggagaa
     3661 ttaggaggta tctatgcttg atataattac tacagaattt caaaaaataa aaagatataa
     3721 tattctatgg attggtgttg ttgctgttct gttttcagca ctattggcag tttttcaggt
     3781 tgtaagttca cacggcagtg accctctaac gtatgagagc tttgcaaacg gagttatctg
     3841 gaataatttc agtcttagtt ttccatttgg gattacactt attggaggat atcttattaa
     3901 ccgtgaatat acagaccaaa ccttaaaaaa catcctaact gttcctattt cattacgcaa
     3961 attgctagtg ggaaaattaa ttgcggttgg aggaattact gtattgttaa gtcttttcag
     4021 cttcttatgc acatttattt tggggttgtt attttgcaat atcaatatat ctgttatact
     4081 cgttgtaaaa tccttagttc agattttgtc tgttaatctg tattgttatg ttgctgtttt
     4141 gccgattatt gcttattttg gtcgtaaaca aaatgcattt ctgacgggtg tagggattgc
     4201 atttgtatac ggtttttgtg gtgtgtttgt tgctggaaga aatctgacag acttttatcc
     4261 aatcacagca ggtcttggtc tagttggata caacaatggt gctggtactg tttatcagcc
     4321 acttatcggt gctatgactt tgattgtaat tctaatcttg acaactattc tacttgtatt
     4381 tacacctaat tatgataaag ttatgaccat atctaagaaa aaagggataa agcataaaaa
     4441 gtattctcat aaataacata aatattgttt ctattattta agttgctaga cagtgaaaag
     4501 aaaaaatctg tcgtacagtg gctatatgct catgtattca agaagctaca gttttgattt
     4561 ttcataactg tagcttcttt ttgtatttat taaatcacga ctatcataat acttgcaata
     4621 ggtatagtaa caagagagag tactgtcata tgtgttttat atattacatt aagtatattc
     4681 taaaatatat gttttaaata atatatttga aaataggaat atttaaataa aaaaaataat
     4741 attatatata gttgacaaaa attggcaaat gatatactat ataaatatag aaatagtaat
     4801 cattactatt attaaaaagg gaggaattaa ttatgaactt aaaaggaact aaaacagaaa
     4861 agaatttaat ggcaggattt gcaggagagt cagaagcaag aaataaatat acatactacg
     4921 cttcaaaagc aagaaaagaa ggttataatc aaatagctgc tatatttgaa gaaactgcta
     4981 acaatgaaaa ggaacatgct aaattatggt ttaaactttt acatgatggt atgccttcaa
     5041 cagaggaaaa tttaaaggat gcagcagcag gtgaaaacta tgaatggact gatatgtatg
     5101 caaaatttgc taaagaagct agagaagaag gatttgacaa gatagcttat ttatttgaag
     5161 ctgttggaaa aatagagaaa gagcatgaag aaagatactt aaagctttta gaaaacttaa
     5221 atgaaggaaa aatatttaaa agagatgaag aagttgtttg gcaatgtcaa aactgtggtc
     5281 atgtgtatgt aggtactgaa gcaccagaaa aatgcccagt atgtgaccat ccaaaagcat
     5341 attttaatat aaaagctgaa aattattaaa aattatcatt attaaaaagg aatgttaact
     5401 atgaaatttt ctaaacaacg agaactgatt ttaaatgaaa tattaaataa tccggttcat
     5461 cctactgcgg attacctata tgaaaactta aaaaaagata atccgaattt aagcttagga
     5521 actgtgtata gaaatttagc tcagctaacg gaacatggct ttataagaaa ggttagtatt
     5581 ccagggtatc cagatagatt tgatggtcga atagataatc actatcatat catatgtgaa
     5641 gtatgtggag aggtatacga tttagaatcc gaagttctta ataatttaca agaattaata
     5701 tctgaggaga cagacataaa aataacatct tataacataa gttttaaagg gatttgtaat
     5761 aattgtaaaa ggtgtagcca agtaggttag ttttcaaatt ctaaaaataa ttaatgaggt
     5821 gagtttatgt gtagtgaaca aaaatttttt atatgtgaga cttgtggaaa ccttgtagga
     5881 atgatacaaa gtggtggtgt acctattttc tgttgtggaa aacctatgaa ggaacttgtt
     5941 cctaatacaa cagatgcagc agttgaaaaa catgtacctg taattgaagt ggatggaaat
     6001 aatgttactg ttaaagtaag tagcacaact catccaatga ctaaagaaca tcatatagca
     6061 tgggtgtatc ttatgactga acaaggtggt caacgcaaat gtctagcagt agatggagaa
     6121 cctgtagtta aatttgcttt gaatgatgat gataaagtga tttctgccta tgcttactgt
     6181 aatttacatg gattatggaa ggctgaactg taatatttaa ataaattgag tcaaaaagga
     6241 ggctataaat gtattttcat acaaatagcc tcctataaat ttatataaat tcaagtttta
     6301 attttgttta atgtcaatat aatataagga gaaaataata tgtcaattta taaatgtagt
     6361 gtttgtggat atatttatga tgaatctaag aatgataaaa catgggatga attaagtgaa
     6421 gattgggaat gccctgtatg tacaaaaggt cgtagttatt ttggaaagat aagtactgtt
     6481 tactatgaag aagatgaaaa gatagcagaa gacatagttg aagatgaatc taaattaaat
     6541 actgaaaaag agggagattt aaattactta agtacatatt taagaagaga tgatgaagtt
     6601 gagaaacata tggatataat tcacgaaatg gctgttacag gaaaatctat aatagaacct
     6661 atgagaacta aactaccagt aatttcttgg gatgatattt taattatggg tgctcaatta
     6721 aatccattac ctttaaatga gcatgatgaa gtaaatacaa caacaataat aggaaaaaaa
     6781 gcaaagaaac ctatgattat agaaaatcca gtttatattt cccacatgtc atttggagct
     6841 ttgtctaaag aactaaaaat agctttagca aagggtgcag cccaaaataa gacagctatg
     6901 tgtagtggtg aaggaggtat attaccagaa gagaaggaag cttcatacaa gtatattttt
     6961 gagtatgtac caaataaata tagtgtaact gaagaaaatc ttaaaaattc tgatgctata
     7021 gagataaaaa ttggtcaagg aacaaaacct ggtatgggag gtcatcttcc tggcgaaaaa
     7081 gtaactgaag aaatagcaaa agtaagaaat aagcctgtag gtcaagatgt aataagtcca
     7141 tcttgttttg aggaaataca atccaaagag gatttaaaga aacttgtaga tgaacttaga
     7201 gaagtttcag aagggcgtcc aataggtgtt aaaatttcag caggtcatat tgaaaaagat
     7261 atggaattta tagcttatgc taaacctgat tttgtaacta ttgatggtcg tggtggagct
     7321 actggagcaa gtccaaagtt gcttaaagat gcaacttcaa ttccaacaat atttgctctt
     7381 tatcgtgcta gaaaatacat agatacacat ggattagata tagatttggt tataacaggt
     7441 gggcttcgta tatctactga ttttgctaag gcaattgcta tgggggctga tgcagttgct
     7501 attgcaagtt ctgctcttat ggcagcggcg tgtcaacaat atcgtatatg tggttctggt
     7561 aagtgtcctg taggagtagc tactcaggat gaggaattga gaaaaagatt acatatagaa
     7621 aatagtgcta atagagtggc aaacttttta aatgtaagtt tagaagaact taagactttt
     7681 gctagaattt ctggtcataa agatatacat gatttaagtg ttgatgattt atatactgtt
     7741 aactctgaaa tatcaaatta tacaaacata cagcatgtat agattgaagt ttgaaactat
     7801 tagattttag acagctataa aagtaaatga taaatatatt ttataaaaga gagtgtctta
     7861 aaatgaactt tttagttcat aagtcactct tttttgtatg aagccaaata tatttatcat
     7921 tttagcaatg aaaaagaatc ttatctcttt attttgagat agactccttc tatttatgaa
     7981 aattaaagat attaaattgt aaaatattct gtgatgtcgt actattaaac taaagattct
     8041 gtactttaaa gtaaataaaa atttaaggag tgattattat ggaaagattg aagatacaag
     8101 ttttagtcga caataatact tatattgata gatactttgt tggagaacca gcagtaagct
     8161 attatattga gattgatgga aataggattt tgtttgactc agggtattca gatgtattca
     8221 tatcaaacgc tgaaaaatta aatatagatt taggaaattt aactcatgtt gttttttctc
     8281 atgggcataa tgaccataca agaggtatcc agtttttaga aaataggtat gatttatcta
     8341 cagttgaact tatttcacat cctaactgtt ttatacctag aaaacatgga gagaaaagca
     8401 taggagctcc attttctgct gaggaaatta aaaatatttt tatgtataat cctaaggata
     8461 agccatttaa tctaagcaag aattgtgtgt ttttaggtga aataccttct ataaatgatt
     8521 ttgaaaaaag agctaaaatt ggtaaatgta aaataggaga tttgtgggaa gatgattatg
     8581 ttttagatga ttctgctata gtttgtaaaa ctgataaagg tatttttatt gttacaggat
     8641 gttctcatag tggaatttgt aatatagtag agtatgctaa aaaggtatgt ggagatgata
     8701 gagttattgg tatactaggt ggttttcatc tttttgaatt gaataatagg ttagatagta
     8761 caatacaata tttggataaa gaagaaataa gaatgttgta cccttgtcac tgtgtttctt
     8821 ttaaggtaaa agcgaagatg aatgaaatat tgaatataaa tgaagttgga gtagggctga
     8881 ccataagtat atagtatttg aaagtatata agatttaagt tgaaggttaa aaagatattt
     8941 tctatagaaa atatcttttt tgttacgttt attttaagag aaatcaatat acttttatat
     9001 gaaatattgt aaaaaaattt atttttaatg agaatatatt ttcattaata aacaatttta
     9061 ttatgtatta ataatgttag aataaatata aataaatcaa agttacttac attacagtat
     9121 attttttcac aaaatatatt gatttatt
//
LOCUS       DBGJFU010000304         8726 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.guided.150, whole genome shotgun sequence.
ACCESSION   DBGJFU010000304 DBGJFU010000000
VERSION     DBGJFU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.guided.150"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            complement(26..1798)
                     /locus_tag="ACOIAS_003871"
     CDS             complement(26..1798)
                     /locus_tag="ACOIAS_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HGZ2703491.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(1812..2741)
                     /locus_tag="ACOIAS_003872"
     CDS             complement(1812..2741)
                     /locus_tag="ACOIAS_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGZ2703492.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            2970..3158
                     /locus_tag="ACOIAS_003873"
     CDS             2970..3158
                     /locus_tag="ACOIAS_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGZ2703493.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            3151..3852
                     /locus_tag="ACOIAS_003874"
     CDS             3151..3852
                     /locus_tag="ACOIAS_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2703494.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3854..4777
                     /locus_tag="ACOIAS_003875"
     CDS             3854..4777
                     /locus_tag="ACOIAS_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGZ2703495.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            4934..5857
                     /locus_tag="ACOIAS_003876"
     CDS             4934..5857
                     /locus_tag="ACOIAS_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703496.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5850..6587
                     /locus_tag="ACOIAS_003877"
     CDS             5850..6587
                     /locus_tag="ACOIAS_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703497.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            6598..7371
                     /locus_tag="ACOIAS_003878"
     CDS             6598..7371
                     /locus_tag="ACOIAS_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGZ2703498.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(7912..8520)
                     /locus_tag="ACOIAS_003879"
     CDS             complement(7912..8520)
                     /locus_tag="ACOIAS_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGZ2703499.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         2888 a         1446 c         1256 g         3136 t
ORIGIN      
        1 atataaattc ttttttgtaa gtttattaat ttctaattat cttctttaaa tacattgaaa
       61 ctacatcaga aaatctagtt atatctcttg aatatataaa atcatttcca tatattaatc
      121 tttcagcttc aagttctgat tcttttcctg taaatactcc taatactaat atattttgct
      181 ttctagcttt tctcacttca ttggctgtat ctctcaaacc aactataccc ctataagcct
      241 tttctcctct taatgttctc tcactacttt ttcctatctt tacatcattt ggtcttccat
      301 cactgagaac tatcaggatt ttattttctt catctctatt tattagacca ctacatatag
      361 attttattgc caaaccatct ctattattac cagcacaaaa atattcaaat atattttcac
      421 tattttttat actatcatca taatctttaa atctttttaa tattgtatag tctaaaaaac
      481 tagaaaatcc catcactcta tttggtatgc caacttctgt tagagcattt gcaattatgt
      541 aagcttgaat tgccacattt ccctgatttc tactttgaga gccacttgca tccaataata
      601 tatcaataac gtatttccct ttttcatttt ctatatcttt ataaaatatt ttattattat
      661 tacttctacc aactctccaa gctctattgg ctaatatagt accacaatca gaatatactc
      721 tacttttagc attttcttct attaaaattc ttgatagact ttcttttaat ctctttattt
      781 gctgtctata cattctagca tgttctctaa attttcctaa attattttct ttttgtcttg
      841 ttacatattt tagttgaaat acattattac actcacttct aagaactcca tctgtaaagt
      901 gtattctaca accttcatga acatttctac aattttgtgt ctcaattttt ttaatttcac
      961 ctttactcaa ataagttttt ccataataat gttctatttt tgaatatatc ttatttgcag
     1021 tgtcttcatc aacatatata actcggtctg ctgaattgct acatttagaa tcatcaaaat
     1081 cccctacacc ttctacaagc atggatgaag atattgaatc tatatcactt tcaataatat
     1141 tttcttcttc atcactataa agttcttcat acatgaaatc agcaaatgta tcaaaattta
     1201 cttctacact ttctacatta ttttcatcaa taagactatt ttcttctcca tttaatatgt
     1261 attcaaaata agttaaatat ataacatcta atgcttttaa aatctccata atatcttgat
     1321 tggtatctat atttcttata tcattaacta ttcttctagt catactatct aatgctgggt
     1381 gcttgtccat actataaagt acaaatgtgt atttaatttt atccataata ttatcattgt
     1441 gtattttggt aaaacgtgaa acaatatctt tataagcatt tcttcttatg tcttctatcc
     1501 ctggcctatc tttaatgacc ttttcttcaa ctattgtatt taatataact tgaattagac
     1561 ccagtataat atctttgttg tatccttttc ttattctatt tgtaagatat tttttaacta
     1621 tatcccaatc tacatacttt cttctagcac ctgcaattat tgcaaaatat agacctattt
     1681 cttttgaaga gtaatctttt tctgatatat ctatactttc attgtaatca ccagctattg
     1741 tccatgataa gttattagct ctattttcaa attcataatc atcatatatc catttcataa
     1801 tctcacctat attaaatatg acttattgga aacacatcta tactctccca tttattaggt
     1861 attctagttt taattacatc tccaaccatt tctttctcat atacatcaaa agtctttcct
     1921 gtaagtccca tattaattgc aagagttggt tttaatcctc ttcttatagt cttaagagat
     1981 gccataagac cacgtaaatc aatagcttta ctagatatct ctccattttg agacttcaat
     2041 tgtaaatcta aaaatattcc tgcaaagtgt attagttttt cttcatcagc atctgaaaat
     2101 tcatttttta aaatcatcat taacttatct tcttctatag gaggaatatc tattaccata
     2161 aatctagata ccagtgcttc attaagttct tttgttcctg catattcata attcatagtc
     2221 ccaataaatc ttgttgctgg atgtagattg actctctcat acccaggtac atcaattatt
     2281 cttctatagt ctaaagctga atgtaccaca actatagcat cattttttgc catattaatt
     2341 tcatcaaaca ccccaaaacc tccattaata gcacactcat aaactgagcc tcttctaagt
     2401 ttaacttcat tgtctataaa tgtatctgta cctataagtg ttgaactgtc tgtatttata
     2461 tgaaatgatg tattccactg aggtctatta aatatttccc ctagattatc agcaagtaag
     2521 ttttttcccg ttgcctttgg tcccgataat aatatatttt cttcttctaa aatagctgct
     2581 atacacatag acaagatatc tttaccataa aaataagttt tagactttgt tactctgtat
     2641 tctaaatctt tttctaatcc ataataattt ctaaaataca gtacatcttc aataagtttt
     2701 tcatctaccc cttgcaccct tagattatct attatatcca tatttttacc ccttttatat
     2761 aaatagtatt tgtttcattt cttatcacca ttttagcata aaaggatata agctaaaatt
     2821 gattttttaa tctcccccat tttaagtaaa accatgcacc attttatcac tacaaaatta
     2881 agagtactac ttactgatat ttcttgattt tattcgtcta tacgtatata atatatactg
     2941 ataaattatg aggaaaggat gattgaaata tggaatatat gtcttgccca gaagtagcaa
     3001 aaaaatgggg tatttctgaa agaagagtac aaaaactatg tgaagaaagt cgcataccag
     3061 gagtttcaaa actcgggtat atgtggctga taccaaagaa cgcagaaaag cctgttgatg
     3121 gtagaacaaa aagaagaaag gaagtacaat atgaatagcg ttttgattat agatgatgac
     3181 aaagaacttt gtgctcttat gaaaaaatgt gttgagcaag aaaatttatc tgctattact
     3241 gcacatggtg gtgcagaagg tctacattta gtcgataaaa atagacacac actctctctt
     3301 gtaattttgg acataatgat gccagatata gacggttttc aagttctgaa aaaaattaga
     3361 gaaacaagta atattccagt aattatgctt actgctaaaa atgacgagga tgataaagtt
     3421 tccggactac gtttaggtgc tgacgattac ctaacaaaac cgttcagcat aaacgaactt
     3481 atggctcgtg tcaattctct tattcgtcgg tatactatgc taaaccctgc ttttataaca
     3541 gacacagatt tcataataat aaatggcatg attattgaca agacaaaccg aattgtttct
     3601 gtcaataata ttcctgttca acttacaagc aaagaatttg atttacttgc ttttctggct
     3661 tctaataagg gacgggtgtt taccaaaaaa cagatttata cacaagtatg ggaagaagaa
     3721 tatgcttttg acgatagtaa catcatgtct ttcatcagta aattgagaaa aaagattgaa
     3781 ccagacccca accatccatt ttatattttg acagttcgtg gtgtcggtta tcgttttaat
     3841 aaggaggctt aacatgaata caagtgtcta tctgcttttg atattggctc ttagcctgat
     3901 tattatagcg taccttgttt ctgtcctgct acgtgtccga atacaactta cacttataaa
     3961 agatgcttta gaggatataa aaaatggaaa ccttaatcga cgtattttaa cagatgaaaa
     4021 cgacattaca aaacaaattt gctatgatat taacgaaata gcaatcaata gccaatttca
     4081 acttattcag caaaaacaat cagaacaagc ctacaaacag ctaatgacaa gtctctccca
     4141 tgatgtaaaa actccacttg cttctttggt cggttatttg gaagcagtag aaagtaaaat
     4201 tgttgtagga gaggaaaaag acgagtatat ccatgttgcg tctaataagg ctcattactt
     4261 aaagaatttt gtagaaaatc tatttgaatg ggtaaaattg gattctaagg aacaagtttt
     4321 tcattttgat atttttgact tgaatgaatt atcccgtaat attatatctg attggatacc
     4381 tgttttagaa agcagtcatt ttgaatatga atttgatata ccagaaatag agtacttctt
     4441 acgtatagac gcaaatgcat atacccgtat cattaataac ctattacaaa atattattac
     4501 ccatagtagt ggaaacaaaa tgacattgag aatttttgaa aacaaagagc aagctcaaat
     4561 tataataaca gataatggga aaggtatatc ttcagacaat ctcccacata tttttgaaag
     4621 gttgtaccaa tgcgaccatt cacgagcttc aaagggaaat ggtttaggct tggcgattgc
     4681 aaaggaactt attaacgctc ataagggaaa tattaccgcc aacagcactc ccggtatggg
     4741 aacagaattt acaatattgc ttcctaaagc cctgtaaaag tacgaggctt tcttactcta
     4801 aaacaagata aaagcaagat tatggcaagg ttttggcaag gttcatgttt tataattcta
     4861 attgtagttg attttgacgt tgtatcaact atcttttgta gaaacaaatt tattaaaagt
     4921 gaggaaatta tatatgaatg attttgtgat tgaaacaaag caacttacaa aaatttatgg
     4981 gaatcaaaca gtagttgaca aagtcaatct ccatgtaaaa aaaggccgaa tatatggtct
     5041 gttaggacgc aacggagctg ggaaaaccac aattatgaaa atgattttag gattgacttc
     5101 aatcacgtat ggcgaggtag atgtatttgg agagaatatt aagggacgtg aaaaacaagt
     5161 atatccccgt atcggtgcaa ttatcgaaac gccaggattt tatcctaatc tgacaggaac
     5221 ggagaacttg gagatttttg caaaactgcg tggtacagcc gcccctaatt ctgtaaaaag
     5281 agcattggaa attgtaggct taccttataa aaataaaaag ctgtttagta aatattcact
     5341 tggcatgaaa cagcgacttg gtattgccaa cgctatcctg catgacccag aactgttagt
     5401 tttagatgag ccaaccaatg gtcttgaccc tattggtatt gcggagatga gaaagtttat
     5461 tagggacttg agcgttgagc atggaaagac aattcttatt tccagtcata ttctttcaga
     5521 aatctcattg ttggctgatg atattggaat tatcgaccac ggtgttcttt tggaagaaag
     5581 tagtatgagt gaactagaaa agaaaaacag taaatatatc ctgctacaag tttcagatat
     5641 tccgaagaca tcacttattt tagagcgtca atttaacacg aaggattatt ccgttcaaga
     5701 tgactacacg ttacaccttt ataacactaa tctggatatg ggtgaaatca ataaagcact
     5761 tgtaatgcag aatgtaactg ttattagttc tcaactttgt aatgatactt tagaggacta
     5821 ctttaaaaaa atcacaggag gagagggcat tgcttaaact tattcaaaca gaatttctta
     5881 aactgcgtcg taggaaactt atttggctga tgttgttgtc tgctctcatt atgccgtttt
     5941 tcgcactact atacttcaat tattttggta aaacaggtgt tgagccaata aagttttata
     6001 aatggtctgc atttggttat acactttgga ttattttgcc tgttgttttg ggtatatttt
     6061 gcaccatgct tatacacgaa gaaaaccagt atgatatgct caaacagctt tggattgtac
     6121 ctattagtaa aatgggatac ttttttagta aatttttcgt tgttttgatt tactctattt
     6181 gttttatgct gattacagcc gttgcttctg tgttatttag tgtactttct ggatatgtcg
     6241 tatttagctg ggcaagcatt ttatatttgt tgaaaaaatg tttagaaatt ggtgtaatca
     6301 ctgcttttgt aatgttgccg attttggcaa tcgctacatc tcaaaaagga tacattctcc
     6361 caatttgtat cacattggta tatgcctttt ccggttttgt tataatgaca ataaatatgt
     6421 atttacaccc attatccagt atgggagtta ttatcatgag aaataaagac attccaggtc
     6481 ttatattttc acaggaaatt aatgtcccat tagcatttct ttgtatttgt atttggagta
     6541 ttgtatcagt attgtttgca aatattgcac taggaagaag aaagtgaggt gtagataatg
     6601 tggaaattac tttgggctga atgtcaaaaa attcgtcgtt caaaaattgt ttggattaca
     6661 gtttttgcat ccattatgat agcagtcata gtttttgcag gggggcagga tgtatataat
     6721 gacccagatt tacattatgg cttaaagagc gtttatgacg gttcacggta tatagacaat
     6781 gcaggttggt atatggacgc agtacaacct tggtcaactt tttttgttct tcctgctgtc
     6841 attgccctat taggaagcta tattatttgt cgtgaagaag aggaggacac tattaaatcc
     6901 ctgcgtctta ttcccataaa tgaaacaaat ttaaccatag caaaaatgat tattactttt
     6961 atattcagta tacttcttta tttgttactc tttactataa catttttgac tgaatccgtt
     7021 ttacattttt ccgatttatc gacaaaattg gttttaagtt gcatgaaaga gtattttctt
     7081 gatggcatag gtgtattctt tgcaatttct cccattattg ccttggtatc gcgcatgaaa
     7141 aaaggatatt ggcttgcatt agtattcaca gaagtttatt ctattgctgg actatttgcg
     7201 ggtatgtcaa atgtcttaca aacctttttt cctattgcgg caatatttaa tatttctgga
     7261 tactatataa ctactggaga aaaaattatg gggagtatca tgagtttatt actttgtgcc
     7321 tgcctttctg catttatact taaagggcta aaacacaatg aaaaaaattg aaataggtat
     7381 atcaaaatga aaaagataaa aaaactgatt attctaattg tattcttgtt tctttttggc
     7441 atgttacact gttatactga atggaaattc aacaataacc cacctaaagg ggtaatagaa
     7501 agaataaatg agtggactga taagattgga ctggattagt aacttatgtt aattatagtg
     7561 aatgtaatat tagcaagtta ttgtttcgta ttttaatact ataactttaa atatttggtt
     7621 ttaatcttcc aaacgaatat aaaaaaactg tcacagaggc aacatatttt tgaatattta
     7681 taaagtggta gttttgattt tcaaagctac cgccttttct tctatataag caaacttatc
     7741 ctcaacaaag tgtaaaacat ggtgcatttt aagaaaaaag taccacagac acaagcaata
     7801 aaagaaacgc cgagaaccta caaaaacgta gtgcttatgc agatttacga cgccttctat
     7861 tttctcaacc gtcttaatga ccttgtacaa aagaaatttt tctaatattt tttataatct
     7921 tatcttgtga tgttttttat gtataaatga cttaataaca aatccaataa aaattatgga
     7981 tattggtata attagaacga ggaatttata atcattataa tgtgacatta ttcttaccca
     8041 actctcagaa aaaatatatc ctatactgat taatgaagta ttccatatgg ttattcccac
     8101 gatagatgat attacaaaag ttataatatt ttgacctgct atacctgcta taaaagatat
     8161 gtatgttcta cataaaggga ttaatctaca tacacaaaca gaaatagaag agtatttatt
     8221 aaaataatct tgagatgcaa atattccttt ctttgccttt ggacatatct ctattatttt
     8281 atttatgata gatttacctc cccaatttcc cactgtataa caaaatatcg aaccaagtat
     8341 acctgcaatt atacttaaaa ttaaaattgt cagaaatcca aaatgatttc ttgcagcaat
     8401 tgctccacat aaaggtaaaa caacttcgct aggtaatgga aaacaagcat actcaatcat
     8461 aattaataca aatacagaga ttaatccata ttgtaaaata aaactattga ttacagacac
     8521 ttacttatct atctcctttc ttatttaaag gttttaaggg tacatgttct aaggattctc
     8581 accctatatt taaagaatca atctctttaa actattatat gtatttacac aaagcattta
     8641 taacatactt taaaaatagt accttaaaat aagaaactta ataaataaaa aaagattacg
     8701 tggctacgtc ctactctccc aggcag
//
LOCUS       DBGJFU010000305         6923 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.guided.169, whole genome shotgun sequence.
ACCESSION   DBGJFU010000305 DBGJFU010000000
VERSION     DBGJFU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.guided.169"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="ACOIAS_003880"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="ACOIAS_003880"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HGZ2703500.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="ACOIAS_003881"
     CDS             1575..2258
                     /locus_tag="ACOIAS_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGZ2703501.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="ACOIAS_003882"
     CDS             2255..4915
                     /locus_tag="ACOIAS_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGZ2703502.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="ACOIAS_003883"
     CDS             complement(5096..6277)
                     /locus_tag="ACOIAS_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2703503.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..>6923)
                     /locus_tag="ACOIAS_003884"
     CDS             complement(6277..>6923)
                     /locus_tag="ACOIAS_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2703504.1"
                     /translation="LEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKTIDLILL
                     DLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPFLLSVLQ
                     SRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSLMENKNR
                     AMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRMEDS"
BASE COUNT         2587 a          876 c         1117 g         2343 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtattacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaaggc gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atataaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataag
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggatatag aatcttaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcagttagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctatagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggc tgactgaaaa agatgcatca aaagaacttg cgaaaaatgg
     3901 aggatttaaa agtatagttt catctaaaaa atactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttataa tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtacagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaagtaa
     5701 ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatat aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ctgtcctcca ttctatatcc
     6301 tatgcctctt aacgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggactgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca tcatcttcta aaa
//
LOCUS       DBGJFU010000306         3294 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.guided.234, whole genome shotgun sequence.
ACCESSION   DBGJFU010000306 DBGJFU010000000
VERSION     DBGJFU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.guided.234"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            <1..303
                     /locus_tag="ACOIAS_003885"
     CDS             <1..303
                     /locus_tag="ACOIAS_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGZ2703505.1"
                     /translation="NIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISV
                     IAINGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            434..1063
                     /locus_tag="ACOIAS_003886"
     CDS             434..1063
                     /locus_tag="ACOIAS_003886"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGZ2703506.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            1118..1774
                     /locus_tag="ACOIAS_003887"
     CDS             1118..1774
                     /locus_tag="ACOIAS_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGZ2703507.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            2189..2905
                     /locus_tag="ACOIAS_003888"
     CDS             2189..2905
                     /locus_tag="ACOIAS_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGZ2703508.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            2877..>3294
                     /locus_tag="ACOIAS_003889"
     CDS             2877..>3294
                     /locus_tag="ACOIAS_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGZ2703509.1"
                     /translation="MLDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGG
                     AENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDT
                     YDGSETYYTDDSINADFNENFENWEHFIKASGKNLKY"
BASE COUNT         1352 a          289 c          586 g         1067 t
ORIGIN      
        1 aatataaagc tattatatta tttaaaagaa gatggtatat attctaggat actttctgat
       61 aaagaagaga tgtatatagt tgaaaatgag gaaatttcat tgtatggaaa cccaggggac
      121 actatttctg taatagctat aaatggtgat gctaagggtg taacacttac tggcttagaa
      181 taccctctcg ataattatta tatgaaatac tctgtaccta ttggaatatc aaatgttatg
      241 ttgagtaatt cttgtaagat taaagtagag caaggctgtg tacttgtagt tagaaattta
      301 taaaatttga ttttgacaat tggttttata ttgtagagca gatacaatca agtaaaatat
      361 atttatgatt gtatctgctt tttagtatat aattataaat atataataaa ctttgacata
      421 agggggattt tttgtggtag atagtattat ttttgattta gatggaacat tgtgggattc
      481 aactgaagaa gtatgcaaag tctggcagga tgttttaagt gagcataagg aaatagaatt
      541 aagtgtcaca aaagatttat ttagaagtct tatggggctt tcttttgagg aaatagcaaa
      601 tcgtttattt ccaaatttaa gtgaagaaga aagaatgaat ttattgaatg aatgttcttt
      661 gaaagaatgt gactatttat ctgaaaatgg agctaaatta tttgatgata tagaagatac
      721 tcttaaatta ctatctaaaa aatataaatt atttatagta agtaattgtc aagctggata
      781 tatagaatct tttttaaaag cacataaact agaacagtat tttatagatt ttgaatgtcc
      841 tggtaatact ggtttgcata aaggagaaaa taataaactt ataattgaaa gaaataaatt
      901 aataaatcca atctatgttg gtgatacgca aggtgatgca aatagtgcta agtttgtagg
      961 tatacctttt atatatgcca aatatgggtt tgggaatgta gatgtatatg attattttat
     1021 agattcattt aaagatttgt tagaacatga tatattaaaa taagatgagt ttaaaaatta
     1081 tattaaaata ataaaaaagt tatgggggga tttaaaaatg gaattacaag atactatttt
     1141 taaaagacaa agtgtaagaa agtttaaaaa tcaagatgta tcagatgaag atattttaaa
     1201 aatgataaaa gcagcaggtg cagcaccatc aggcaagaat attcaaaact ggcattttgt
     1261 tgttataaaa cgccgtgatt taatggaaaa gatagcagat gtgataacta aaaagcaaca
     1321 agaaatactt gtagaaatgg ataaagtatc agtggataag gctaatagat ttagaaaatt
     1381 tgttaaaaat tttacactat tttacttaaa agctccagtt ttagtattag tttttacaaa
     1441 ggtatataat ccatcaggat attatgaatt agagcttata gatgcaccta aggagactat
     1501 agataaatta tttataagaa atccaggtat gcagagtcta ggtgctgcaa ttgaaaactt
     1561 tacactatca gcaattgaac ttggatatgg ttcttgttgg ttgacaagtc aaaactatgc
     1621 agcagatgag atagaagctg ttttagaagc agaaactggt tttgaaaagg gagagtattt
     1681 tttaggtgca atgttagctc ttggagtgcc agaggacaat ttgaagagtc catctaaaaa
     1741 accagtagaa gaaatatgta catttataaa ataaaagtca aggacatttt aaaatcgaag
     1801 taaattaaaa acaaaataaa ttaaaaataa atatttaaaa aattaaaaag taggttttat
     1861 tgatattaat tttatatttc aaatcattaa tttaattaat acaataagaa ctactttttt
     1921 ttgtatatta attttaaata tatttaaatc tttagaaaat ctttagaaaa gtatatgtaa
     1981 tatcttaagt ttttaatttt aatattaagt atataaaaca aaatgaaaaa atattaaatt
     2041 taaactagaa ggaggtttct aagatggaca ttaaatacaa ataaccagaa caaaagatat
     2101 aaatatatat ataaatggaa aaacttctat catattgtat aatcatatat gtaaagaaaa
     2161 tttataaatt aatataaagg agattagaat gaattcatat aatattttgg tggtagaaga
     2221 tgaaaaggag atagcagatg ctatagaaat atatctatta aatcaaggat ataatgtttt
     2281 taagggatac aatggattag aaggtcttaa ggttatagaa aaccaagaaa tacatttagc
     2341 tatagttgat ataatgatgc ctaaaatgga tggtataaca cttacaatga agcttagaga
     2401 aaactataat tttccagtaa taatgctatc tgcaaaatca gaagaagttg acaaaataat
     2461 ggggcttaat ataggagcag acgattatgt aacaaaacct tttaagccat tagaactatt
     2521 ggctagagtt aattcacaac ttagaaggta cactaagtat ctaaacatgg ttggaaataa
     2581 agagcaaaag gtagaaagtg acgatagtgt gtttgcaata ggcggattag aattaaatga
     2641 aaatacaaaa gaagttagta ttgatggaaa gtatataaaa acaactccta tagaatttaa
     2701 aatactaagt ttacttatga gaaatgcagg aagagttttt tctgctgatg aaatatatga
     2761 aagagtttgg aatgagaatg ctgtaaatac agatacagtt atggtacatg tgaggaatat
     2821 aagggaaaag atagaaattg acccaaaaaa tcctaaatat ttaaaggtgg tgtggggtgt
     2881 tggatacaaa attgaaaaaa tttaaagata aatatatagt tactattata gtttttatac
     2941 ttatgttatc agcatcatta ggtatgatta gtcaatattc tactatacaa gcaagtgcta
     3001 aaggtggagc agagaatcca tttgaacagg aaagctttgt agatagtata tataaaggaa
     3061 gttatgtttt atatcataat atgaaggaag aacaagaagg taagatgata caaccatcaa
     3121 agctatttat caatgaagaa actatgaact atataaaaaa tgtaagtaaa gaaaaagata
     3181 cttatgatgg ttcagaaact tattatacag atgatagtat taatgcagat tttaatgaaa
     3241 actttgaaaa ttgggagcat ttcattaaag cgtcaggaaa gaatttaaag tact
//
LOCUS       DBGJFU010000307          741 bp    DNA     linear   BCT 20-MAR-2025
DEFINITION  TPA_asm: Clostridioides difficile isolate B47
            SAMN43225850-rid25700633.guided.285, whole genome shotgun sequence.
ACCESSION   DBGJFU010000307 DBGJFU010000000
VERSION     DBGJFU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43225850
            Sequence Read Archive: SRR30483168
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-MAR-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002674330.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002674330.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/17/2025 19:39:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,890
            CDSs (total)                      :: 3,806
            Genes (coding)                    :: 3,774
            CDSs (with protein)               :: 3,774
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 7 of 32
            Pseudo Genes (incomplete)         :: 27 of 32
            Pseudo Genes (internal stop)      :: 7 of 32
            Pseudo Genes (multiple problems)  :: 6 of 32
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43225850-rid25700633.guided.285"
                     /isolate="B47"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2023-05"
                     /collected_by="SSI"
     gene            111..>741
                     /locus_tag="ACOIAS_003890"
     CDS             111..>741
                     /locus_tag="ACOIAS_003890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGZ2703510.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          298 a           84 c          128 g          231 t
ORIGIN      
        1 ggacgccatt atcttatgaa gattcttaac aaggagtgat tctgtgaaga gatgtattgt
       61 agtagtggaa tagtcaaaaa cataatatat attaaaggag agatttaaat atgactattc
      121 caagttcaaa taaggtatat ccaagaagta atgattatca aactgtatat cttaaaaatg
      181 taataacaag ggaaaatata gaagttggag attacacaat atataatgac ttttataatg
      241 acccaagaga ttttgagaaa aacaatgtat tgtatcatta ccctataaac aatgataaat
      301 taataattgg taagttttgc tcaatagcat gtaatgcaaa atttctaatg actagtggaa
      361 atcactcaat gaaatcaaaa tccacatata cttttcccat cttttatgaa gaatggaaac
      421 tcgatatcaa ccatataacg gacgcttggg acaataaggg taacattata attggaaatg
      481 atgtgtggat aggttatgat tctataatta tgtcaggtgt aaatattggt gatggtgcaa
      541 ttattggaac aagagcagta gttactaatg acgttccacc gtattctatt gttggaggag
      601 tacctgcaaa aattataaaa aagagattta ataatgatac aatttcaaaa ttattagaaa
      661 ttaaatggtg ggattggcca tataaaaaaa ttcaatcaaa cattaagcat attcaatcag
      721 gaaatattga taaattagtt t
//
